LOCUS       DATNGP010000001         7118 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_3712267, whole genome shotgun sequence.
ACCESSION   DATNGP010000001 DATNGP010000000
VERSION     DATNGP010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 7118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7118
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3712267"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1244
                     /locus_tag="VN778_00005"
     CDS             <1..1244
                     /locus_tag="VN778_00005"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXR49398.1"
                     /translation="INLRSSVPARLTQTIKQTFWSLRSRNFRLFFIGQSVSNSGNWLT
                     NVALTLLVLHLTGSGLVIGILTACQFGPILLLSAWGGAIADRYNKRNLLFITQSLEML
                     ESVALAALAFTHSPSIVALFAVATVGGILLAFDNPLRRSFVTEMVPVEDIPNAVVLYS
                     TVVNVARIIGPALAGALIVTVGYGWCFIVDAISYLAVLACLWLMRPSELRVAPDRPSS
                     KGLIRAGISYVLAVPVLWITFVMLLIIGSFAYNFTVTLPLLVTKSLHGGAGTFTLIYS
                     TFGIGALCGALLTANRRLVSVRAVVNGALMMGITMFAIAMAPTVLIMAPIAFALGITS
                     IIYMTSTTSLVQVRAKPSMHGRVLALQTVLLVGTTPIGGPLLGWLADTAGARTPIAIG
                     GIACIAAGLFGYVAIKNFSSI"
     gene            1313..2212
                     /locus_tag="VN778_00010"
     CDS             1313..2212
                     /locus_tag="VN778_00010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49399.1"
                     /translation="MWFALSVLALLMLSGRRTAEKQAAIGIDSMAMAWLQQGVAMPLI
                     IVSLFFAKFYWPSQLPAFFWELLALYVALQAAYLYCYYRAVAIADISYVGSLMTLFSV
                     GNMVGAYLILGQVPSVYGVIGAASIMLGAWIISRAKLRGGGESIAAHKTALTLVVIAI
                     ILCSVFSNIEVKMLRMSNPTSYNFYTSLLTVPFVILVSMLILRSRRQSNRIYWQNVKT
                     SIQLNLWPLAIVGVTYTINMLATYQAKVMAPNQAYVGSIKAASVLPIVILGLLFFHEK
                     VTPKHWLGVACMLVGLAFMAVNL"
     gene            2248..2850
                     /locus_tag="VN778_00015"
     CDS             2248..2850
                     /locus_tag="VN778_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002745149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HXR49400.1"
                     /translation="MRKIVVFTMLSLDGVMQAPGGPEEDTSGGFKYGGWTAPYADESF
                     GPILYKELSVPFDLLLGRKTYEIFAAYWPKQTGPIADPFNKATKYVVSGQGIDLTWKG
                     SVLISDDVVAKIKALKAEDGPMLQVHGSAKLIQTLLKNDLIDELRLRIYPVTLAEGKR
                     LFAEGTIPAAFELLDSKVLSSGVILANYKRSGEVKTGSID"
     gene            3183..3791
                     /locus_tag="VN778_00020"
     CDS             3183..3791
                     /locus_tag="VN778_00020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49401.1"
                     /translation="MPLILRRIAVGVLITCALSAVGASLASATPDNSQLLAVVSKHLS
                     MNNKQDKLCQEASAVRRKVIKQFGKRTPGRDICKSGLPSGQKPSKSREEHYLQTLQRM
                     IAPPVASATASSSQSWTVSTSSSSTGGGGLPSCASESGTNYSTGSSNTNSSSGATGRY
                     QIIPSTHVALCSDLGWSPSEQDQCAARIYQAQGSGAWVGCGG"
     gene            3934..4509
                     /locus_tag="VN778_00025"
     CDS             3934..4509
                     /locus_tag="VN778_00025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49402.1"
                     /translation="MGEYFNEKRTRALMIAGLGAAAFAGYHLVKDKTGFKMSLGLPER
                     PATMPADFQERLASVNDKVNELIEGSAAFNLEGNNELHKYIVPPVAFVFSESQERFAE
                     IVNHGTSLSITIVPARKKEGSLKTGGEPTNNAWDGKGHQMYWGVTPTGIRTELYTFGS
                     DGLEPEQALDRIHNLVFADDLELMSLDATLS"
     gene            4556..4984
                     /locus_tag="VN778_00030"
     CDS             4556..4984
                     /locus_tag="VN778_00030"
                     /inference="COORDINATES: protein motif:HMM:NF018663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXR49403.1"
                     /translation="MLRCLPFLLFDGNCAEAMTFYHECLGGELSLTKLGDTPMKDMFP
                     PEKHGRLINANLKSGDIEIAATDWMAAPEYDPKFGDTYAIFIVGEGYDELKAVFDKLA
                     QGAQKDRFQDLHELPFGMYGQFYDKFGVQWIFKGDKAAPQ"
     gene            complement(5001..6464)
                     /locus_tag="VN778_00035"
     CDS             complement(5001..6464)
                     /locus_tag="VN778_00035"
                     /inference="COORDINATES: protein motif:HMM:NF015066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neprosin family prolyl endopeptidase"
                     /protein_id="HXR49404.1"
                     /translation="MDNNLQSGVTSSEPVNGIPPTQRPTPLSVGRVAVQPLIVTPSIQ
                     NLSPAAQPPAANQGFGSMEFPQVIVGNLNTPAPIPAKQVHFPYVRLALAVLLLLVLSF
                     AAFGLRQNSKSAALYNQASADIKNGRYSAAAPLLSSASKYFSLPATHGKILKLASQNQ
                     VWLSDEDYLARATALVAQKSFDDATALLAKIGKDFPGYQQVTVLQTAITSDKKLAAAT
                     QPIAKTQTPASGQATPSKAVATTQPHKTPSISTPVVGGGAAPITIPVYTTSGYNYYYA
                     GARQFVNNEGASITFPIERPSVGQSGGSQNHSLMELAMEDNNYSTVEIGWIVDGPQFG
                     DTLPHLFVYHFSNHATTCYNGCGFVQVSRTNYAGEALAAGTSATFKAVFSNNQWQVFY
                     NGDEVGYFPLSSYSDNFTTATIVQVYGEVSTTGNMCIQMGNGIAGNAPGAARLSNFSL
                     IGASASPNLSPYQTASSPYSYGSASYNGVSIGGPGSC"
     gene            complement(6583..>7118)
                     /locus_tag="VN778_00040"
     CDS             complement(6583..>7118)
                     /locus_tag="VN778_00040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49405.1"
                     /translation="PLILGYMSTRIPKLVGKYGFKRFLVAGPLLVAFGLALISRVSVH
                     SSYLADLLPTFILMPLGMGMTFMPIIAAATSGVPGHQSGLASGLITTSQQMGGALGLA
                     ILSGIAASVTASSAYLGQSAALVHGFDRAILAAVVFMLFAASLGVAVIKQPKKRTPRD
                     GEPIRHEALRPSEQFHS"
BASE COUNT         1688 a         1714 c         1962 g         1754 t
ORIGIN      
        1 caataaattt gcgaagtagc gtgcctgcgc gattaactca gacaatcaag cagactttct
       61 ggtcactgcg ctcgcgcaac ttccgcttat tttttatcgg tcaatccgta tcgaatagtg
      121 gtaactggct gaccaatgtc gcgctgacac tactggtact gcatctgact gggagtggct
      181 tggtgatcgg tatcttgact gcctgccagt ttggtccgat tctactgctg tccgcctggg
      241 gcggtgccat agccgatcgt tacaacaaac gcaacctgtt attcattacc cagagcctgg
      301 aaatgcttga atcggtggcc ttggcggcgc tggcctttac gcatagtccg tcgatcgtag
      361 ccttattcgc ggtcgcgaca gtcggaggga ttttgctggc tttcgacaac cctttgcgac
      421 ggtcttttgt aactgaaatg gtacccgtgg aagatattcc gaacgctgtc gtgctctata
      481 gtaccgtcgt caatgtggcg cggatcattg gaccggcctt agctggggca ctgattgtga
      541 ctgtgggtta cggctggtgt tttatagttg acgctattag ctatttagcg gttttggcat
      601 gtctttggct gatgcgaccc agtgagctca gggtggcacc ggacagacca tcgtccaagg
      661 gattgatccg ggccggtatc agttacgtct tagctgtacc ggtgctatgg ataacctttg
      721 ttatgctatt gataatcggc agctttgcgt ataacttcac agtcactttg ccgctactag
      781 taactaaatc gctgcacggc ggcgcgggaa cctttacact tatctactcc acgttcggta
      841 ttggcgcttt gtgcggcgca ttactgacgg ctaatcgtcg gctcgtcagt gttcgcgccg
      901 tggtcaatgg cgccttaatg atggggatta cgatgtttgc aattgcgatg gctccaacag
      961 tcctcataat ggcaccgatc gcctttgcat tgggcataac cagtatcatt tacatgactt
     1021 ctaccacgtc gcttgtgcag gtcagggcta agccctccat gcacggtcgt gtgttggcgc
     1081 tgcagaccgt gttattggtg ggtacgacac caatcggcgg cccattgcta ggttggctgg
     1141 ctgatactgc tggcgcccgt acgccaatag caattggggg tatcgcttgc atagccgccg
     1201 gcctgttcgg ctatgtggca atcaaaaact ttagctccat ataggaaggt aatactaaca
     1261 aaaagttata accatagcta atccggcatt cgctaaatgc tacactggag ccatgtggtt
     1321 cgcgctttct gtcttggcgc tgctaatgct gtctgggagg cggacggctg aaaagcaggc
     1381 ggctattggc atcgacagca tggcgatggc gtggttgcag cagggcgtgg ccatgccgct
     1441 tatcattgtt tcgttgttct ttgcgaaatt ctattggccc agccagctgc cggcgttctt
     1501 ttgggaatta ctggccttgt atgtggcgct ccaggcggcc tacttgtatt gctactaccg
     1561 cgccgtggcg attgccgaca ttagctatgt gggttcgttg atgacactgt tttcagtcgg
     1621 taatatggtg ggcgcttacc taattcttgg tcaggtacca tcagtgtatg gcgttatcgg
     1681 agcagcctcc attatgctgg gagcctggat tatatctcga gccaagttgc gtggtggcgg
     1741 cgagtcgata gcggctcaca aaactgcttt aacgttggtc gtaatcgcca tcatcctctg
     1801 cagtgttttc tcgaatatcg aagtcaaaat gctccgcatg agcaacccca cgagctataa
     1861 cttttacacg tcgctactga ctgtgccatt cgtaatcctg gtgtccatgc tcatactcag
     1921 gagtcgccgc caaagcaata ggatttattg gcagaatgta aaaacaagca tccagcttaa
     1981 cctttggccg ctagcgattg taggtgttac gtacactata aatatgttag cgacttatca
     2041 agcgaaggta atggcgccaa accaggccta cgtgggctct attaaggcag cgtctgtgct
     2101 accgatcgtg attttaggcc tgctattctt ccacgaaaag gtgacgccca agcactggct
     2161 tggcgtcgcc tgcatgctcg tgggcctggc gttcatggct gttaatttgt agcttaaata
     2221 ttcgttagga tttgttacga tataagtatg agaaaaatag ttgttttcac aatgctcagc
     2281 cttgacgggg tcatgcaggc gcccggtggc ccagaagagg acacatcggg gggttttaag
     2341 tatggtggct ggactgcgcc atacgcggat gaatcgttcg gcccgatact ctacaaagaa
     2401 ttaagtgtgc cgtttgactt gctgctcggc aggaaaacgt atgagatttt tgctgcgtat
     2461 tggcctaagc aaactggtcc gattgctgac ccgtttaata aggctacaaa atacgtggtg
     2521 tcaggtcagg gtattgacct aacttggaaa ggatcagtac ttataagtga tgacgtggtg
     2581 gctaaaatca aagcgctgaa agcagaggac ggtccgatgc tacaggtaca cggcagcgcg
     2641 aagcttattc aaacactcct gaagaatgac ctgatcgacg aacttcgact cagaatctat
     2701 cctgtcacac ttgctgaggg caagcgcctg ttcgccgagg ggacaatccc ggccgctttt
     2761 gaattattag actctaaagt gttatcgagc ggtgtgattt tagccaacta taaaagatcg
     2821 ggcgaagtta aaactggctc aattgattga tatttgcacc tgtcttcatt aattgatgcc
     2881 gcaatagcgg ttgaagagag gacttctata gcaaaagcag cttaagcccg ccggtgttac
     2941 ctgtgggtta attcacagaa agctcgagtc gcttttacca taatactcag gacgcggttg
     3001 tcggactggt ccgaacgctg ttgtctcgca gggagttggg cgctcaagct cgacgccaac
     3061 ctgagaggct cggactttaa gcttactcgt cggaaagcga cgatgatagc ttataagctg
     3121 acagctgaaa cccagatgag ctggtggttg gctcgtcgca ccttaaggag ggaaaggcag
     3181 aaatgcccct aatcctaagg cggattgccg tgggcgtgct aataacgtgc gctctgtcgg
     3241 ctgttggtgc ttcgcttgcg tcggcaacgc cggataacag ccaattactg gctgtggtga
     3301 gcaagcacct gagcatgaac aacaagcagg acaagctctg ccaggaggcg agtgccgtcc
     3361 gtaggaaagt gatcaagcag ttcggcaaac gaacgccggg acgagatatc tgcaagagtg
     3421 gtctcccaag cggccaaaag ccgtccaaaa gccgggagga gcattacttg caaacactgc
     3481 agcgcatgat cgccccgccg gtagcaagtg ctacagcttc gtcttctcag tcctggactg
     3541 tttcgacgag ctctagctca actggcggcg gcggtttgcc aagttgtgcg tccgaatcag
     3601 gtacgaacta ctcaaccggg tcaagcaaca ccaactcatc gagcggtgct accgggcgat
     3661 accagatcat tccgagtacg cacgttgcat tgtgctcgga ccttggctgg tcgccgtccg
     3721 aacaagacca atgcgccgcg cgcatctatc aagctcaagg ctccggcgcc tgggttggct
     3781 gcggaggcta atgtaaccag ataactcaga cagtgcttgg ggagcggtga gtccgcttgc
     3841 cgctcccctt gagcacacct atctgacaat cgcgtatttc ctttgacaga tacacgaatt
     3901 cttgatataa atagtaatat agggagttta tcagtgggcg aatatttcaa cgagaaaaga
     3961 accagagctt tgatgattgc aggccttggg gcggctgctt ttgccggcta tcacctggta
     4021 aaagataaaa ctggctttaa gatgagtcta ggcttgcctg aacggcccgc tactatgcca
     4081 gccgacttcc aagagcgcct agcttcagta aatgataaag tcaatgagct aattgaggga
     4141 tcggcagctt tcaacctcga gggtaacaat gaactacaca aatacatagt tccgccagta
     4201 gcattcgtgt tcagtgaatc tcaggagcgg tttgccgaaa ttgtgaacca cggaacgagc
     4261 ctctccataa caatcgttcc agctcgtaag aaggaaggat cactaaaaac aggcggcgag
     4321 ccaactaaca atgcctggga cggaaaaggc catcaaatgt attggggagt aacaccaact
     4381 ggcattagaa ctgaactcta tacctttggc agtgatggac tcgaacctga gcaggcgctt
     4441 gatcgtatcc acaatcttgt ctttgctgat gaccttgaac tcatgagcct agatgcaacc
     4501 ttgagctaag cattagtcga ccgacttttt ttgtgacttt tgctattatg ttcgtatgtt
     4561 acgttgtctc ccatttttgt tatttgacgg caactgtgcc gaagccatga cgttttatca
     4621 cgaatgcctg ggcggtgagc tgtcgctgac caagcttggt gacacgccaa tgaaagacat
     4681 gtttccaccc gagaagcacg ggcggctcat caatgccaac ctaaaaagtg gtgatataga
     4741 aatagcagcc accgactgga tggccgctcc cgagtacgac ccaaaattcg gcgacaccta
     4801 tgccatattc atcgtcggcg aaggctatga cgaattgaaa gccgtgttcg ataagctggc
     4861 ccaaggcgcc caaaaagacc ggttccaaga tctgcatgaa ttgccttttg gcatgtacgg
     4921 acagttttat gacaaattcg gcgtacaatg gattttcaaa ggcgacaaag ctgcacctca
     4981 gtaaagtgta aggcagactt ttagcacgaa cccgggccgc cgatggacac accattgtac
     5041 gaggcggatc cgtaactgta gggcgaggaa gctgtttggt aaggcgagag attgggtgag
     5101 gctgacgcgc cgatgaggga aaaattgctg agtcgagccg cgcccggcgc gttgccggca
     5161 atgccgttgc ccatttggat gcacatgtta ccggtcgtgc taacttcgcc gtagacctgg
     5221 acgatggtgg ctgttgtgaa gttgtcagaa taagatgaca aagggaaata tcccacttcg
     5281 tcgccgttat aaaatacctg ccactggttg tttgaaaaaa cggctttgaa ggttgcgctt
     5341 gtgccggccg ctaaagcctc gccggcatag tttgtgcgcg acacctgcac aaaaccgcaa
     5401 ccgttataac aagttgttgc atggttacta aaatgatata caaacaaatg cggcaaagtg
     5461 tcgccaaact gcggtccgtc gactatccag ccaatctcga cggtactgta attattatct
     5521 tccatggcca gctccatcag agagtgattc tgactgccgc ccgactgtcc aacagagggg
     5581 cgctcaatcg ggaaagtgat tgaggcgcct tcgttattta cgaattgccg cgcgccggca
     5641 tagtagtagt tataaccaga ggttgtgtac accggaatgg taatgggggc tgcgccgccc
     5701 ccaactaccg gagttgagat agatggagtt ttgtgcggtt gagtagtggc aacggcttta
     5761 ctcggagtag cttggccaga agcaggcgtt tgcgtcttag caatcggctg agtagcggcg
     5821 gcgagctttt tatcgcttgt tattgcggtt tgcagcaccg ttacttgttg gtagcctgga
     5881 aagtccttgc caatttttgc gagcagcgcc gtagcatcgt caaagctttt ctgggccacc
     5941 aaggcagttg cccgagcgag atagtcctca tcggagagcc agacttggtt ttgagaagct
     6001 agcttgagga ttttgccgtg ggtcgcaggc agggaaaagt atttgcttgc actactgagt
     6061 aagggcgccg cggcactata ccttccattt ttaatgtccg cgctggcctg gttatataag
     6121 gccgcacttt tggaattttg tcgcagtcca aatgcagcaa atgacaacac gagtaacaac
     6181 agtacggcga gagctagcct gacatatgga aaatgtacct gcttggcagg aataggcgct
     6241 ggagtattga ggttgccgac aatgacttgc ggaaactcca tggagccgaa accttgattt
     6301 gcagcgggcg gttgcgcggc aggactcagg ttttgaatac taggtgtcac aatgagcggt
     6361 tgtactgcaa ctcttccaac cgatagtgga gtaggcctct gggtgggcgg aataccgttc
     6421 acaggttcgc tcgacgtaac gccgctttgt aaattattgt ccatcgtttt tgtataagct
     6481 gttagctcaa taatagaaga tggcaagaca gtatgcaaat gagccggcgc aggaaaacgc
     6541 cccgccggtg agcgaggcgt tctctgttag cgagccggct cgttagctat ggaactgttc
     6601 gctaggccgt aaggcttcgt gccgaattgg ttcgccgtct cgcggtgtgc gtttcttggg
     6661 ctgcttgatg acggcgacac ccaggctggc cgcgaagagc atgaagacca cagcggctaa
     6721 gatagctcga tcgaaaccat ggacgagggc tgctgactgt cccaagtagg ccgacgaagc
     6781 agtgactgag gccgcgatgc cggacagtat agcgagaccc aaggcgccac ccatttgctg
     6841 ggaagtggta atcagccctg aggctaagcc ggattgatgt ccaggcacgc cagaagtcgc
     6901 tgcagcgatg ataggcatga aggtcatccc catgcccagt ggcataagga tgaatgtcgg
     6961 caacaggtcg gctaggtagc tgctatgcac tgaaacacgg ctgatcagtg ccaggccgaa
     7021 agccaccaag agtggaccgg cgaccaaaaa tcgcttaaag ccgtacttgc caaccagctt
     7081 cggtatgcga gttgacatat agcctaagat tagcggga
//
LOCUS       DATNGP010000002         4685 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_1546280, whole genome shotgun sequence.
ACCESSION   DATNGP010000002 DATNGP010000000
VERSION     DATNGP010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4685)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4685)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4685
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1546280"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..303)
                     /locus_tag="VN778_00045"
     CDS             complement(<1..303)
                     /locus_tag="VN778_00045"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HXR49406.1"
                     /translation="MDEIAAGLIKAKFQGETDSSPATLDLYSHDASMFELRPTLVATP
                     KDVQDVEKLVGVVAAQKKANPKLSLTARSAGTDMSGGAINESIIVDFRKYFTKIEKI"
     gene            362..1447
                     /locus_tag="VN778_00050"
     CDS             362..1447
                     /locus_tag="VN778_00050"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018501495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXR49407.1"
                     /translation="MKIALVGPTHPYKGGIVLHTTELAHRLTKAGHTVEILSWRTQYP
                     FFYPGTQFVPGNKPESPPFPHVKRILSWRNPLGWWRQGRHLRSFDEVIFVWWVPTIQG
                     PVYLSLLRALGKRGPRTMAICHNVLPHEPRLGDKWLARTVLTRVNQVIVHTSEQAETA
                     RQLTTKPVRVITLPFSFPPVPPQNRKQSTHLAQHLLFFGFVRPYKGLDVLLHALADIP
                     DIRLTVAGEFWGSITQYESLVGQLGLNERVVLRNEYIPADDLATLISTCDAVVLPYRH
                     GTATWNTTLAFSYGTPVIATTAGSLASQVRDGVDGLLCKPDDAKSLTAAIRHFYEPRI
                     AAQLRHGVPKITTDADWEAYVEAVTKH"
     gene            1484..2677
                     /locus_tag="VN778_00055"
     CDS             1484..2677
                     /locus_tag="VN778_00055"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXR49408.1"
                     /translation="MPKKERILIVGGGFAGVKAALELAGEQHFEIALLTDSNELRYYP
                     TLYHTATGGKRANSSIALSTIFADKDIELKQGSAATLDRKAKTVTTTDGQALAYDTLI
                     LALGVVTNYFGIPGLQEFSYSIKTQAEVERFKAHLHKQLEDERRPDLNYVIVGAGPTG
                     IELAGALPHYLQHIMANHGMPARKVHIDLIEAAPRLLPRSPKDTSRSVRRHLRKLGVR
                     LYLGSVVQGETADELTVNGKPIRSHTVIWTAGVTNHPFFVANQFVLTKRGKVAVDTYL
                     QAEDSIFVLGDNANTPYSGLAQTALYDGKFVANNLRRRAVGKRFKSYIAKQPISVIPA
                     GPRWAAVEWGVLHFQGRLGWLLREAADLIGFHDLESWPLATKQWLTEFSGQDNCAVCT
                     AAQVN"
     gene            2711..>4685
                     /locus_tag="VN778_00060"
     CDS             2711..>4685
                     /locus_tag="VN778_00060"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HXR49409.1"
                     /translation="MDEVFRAAYKQLNNKQREAVDTIDGPLLVIAGPGTGKTQLLSMR
                     VANIIQQTDTAPSNILCLTFTDNAARNMRERLETIIGQPAYHVAIHTFHSFGGEIISQ
                     YSDYFAGRQLLQQIDDLGRYELLREIFEALPYDNPLGVKVGDEFVHLRSTLDIISWLK
                     QNALTPAEMRTLIAVNRQFMEVMTDELAETFAVTPSPKLLSKYKKFVGTAQKHITGHR
                     HYGFPEYAAECVGELEAAMANTSVDGRYAPAITAWRNKWCVKDAQNRHAFKDAGQNMR
                     KVQAVADVYEQLLERMSELGLYDFDDMIVEVVHALETKPDLRFTLQERYQYLLVDEFQ
                     DTNKAQLRMLLALGNNPVNEGQPNIMTVGDDDQAIYAFQGAEVSNMVAFAEQYPTAKR
                     ITLEENYRSNAHILDASFAVAGQITDRVQSILPDTHKTLVPRANYGTDILQHTVFPSE
                     LAQYDWIADQIKAYLKQGIQPEAIAVIAPRHRYLERLMPYLGNCHIPVAYERREDILE
                     SPIIVQLLTMTRLVTSIADNQQSEVDHLISQILGYDFWGISADDLVKISLECYNKHEH
                     WLPVLLAHENEKLRDITTWFTFLAKQSAIEPLEYMLDILLGNLPDGKDNEYDDLLLPV
                     NRKPWFISPMFEFYFNPERYELATDTYLMLLGQL"
BASE COUNT         1146 a         1253 c         1200 g         1086 t
ORIGIN      
        1 gattttctcg atttttgtaa agtacttcct gaagtcaaca atgatcgatt cattaatagc
       61 acccccggac atatccgtgc cggcgctgcg agccgtcaag ctgagcttgg gattggcttt
      121 cttttgagct gctacaaccc caacaagctt ttcgacgtct tggacatctt taggcgtggc
      181 cactagtgtg ggtcgcagtt cgaacatcga agcgtcgtgg ctgtaaagat caagcgtagc
      241 aggcgagctg tctgtctccc cttgaaactt ggcctttatg aggcccgcgg caatttcatc
      301 cataccataa gcttaagggg tatgggggcc tgaatgcaag ttttgttaca ctgggagcag
      361 catgaaaata gcgctagttg gtccgacaca cccttataaa ggcggcattg tactgcatac
      421 caccgagcta gcgcatcggc ttacaaaagc cggtcacacc gtagagatac tctcctggcg
      481 cacccaatat ccctttttct accccggcac gcaatttgtc cccggcaaca agccggagtc
      541 gccgccattt ccccatgtaa agcgtattct gtcatggaga aacccgcttg gctggtggcg
      601 ccaaggacga catttgcgta gttttgatga ggtaattttt gtttggtggg tgccgaccat
      661 tcaggggccg gtctatctca gtctgctccg tgccctaggc aagcggggac cacgcacgat
      721 ggcgatttgt cataacgttt tgccacacga accccggcta ggtgacaaat ggctagcccg
      781 tactgtcctc acacgcgtca atcaggttat agtgcacacc agcgaacaag ccgaaacggc
      841 tcgtcaactt acgacgaagc cagtccgggt cataactttg ccgtttagtt tccccccggt
      901 accgccccaa aaccgcaagc aatcgacaca ccttgcacaa cacctgttat ttttcggttt
      961 cgtacgcccc tacaaggggt tggatgtgct cctgcacgcg ctagcggata ttccggatat
     1021 tcgcttgacg gtggccggtg aattttgggg aagtatcacg cagtatgagt ccttagtcgg
     1081 acagctcggt ttaaatgaac gcgtggtact gcgtaatgaa tatatcccag ccgacgattt
     1141 ggcaacgctg atcagtacct gcgatgctgt agtgctaccg tatcggcatg gcacggcgac
     1201 ctggaatacc accctggctt tcagctacgg tacaccggtt attgcaacga ctgcgggctc
     1261 tttagccagt caagtgcggg acggcgtcga tggcttgtta tgcaaacctg atgatgccaa
     1321 gtcgctgacc gcagctattc ggcacttcta tgagccgagg attgccgcac agctgcgaca
     1381 tggcgtgccg aaaatcacga ccgatgccga ttgggaggcc tacgtcgaag cagttactaa
     1441 acactagagg ttacaagctt ttccttgtat aatcataagc gatatgccaa agaaagaacg
     1501 aattttaatt gttggcggtg gctttgctgg cgttaaagca gcccttgagc tagccggcga
     1561 gcagcatttc gaaatagcct tactgacgga cagcaatgaa ctgcgctact accctacgct
     1621 atatcacacg gcgaccggcg gtaagcgcgc taactccagc attgcgcttt cgaccatttt
     1681 tgccgacaag gacatagagc tcaaacaagg gagcgcggca actttggatc gcaaagccaa
     1741 aactgttacg acgaccgatg gccaggcgct cgcgtacgac acgctcattt tagctctcgg
     1801 tgtggtaacg aattacttcg gtatccccgg gttgcaggaa ttctcctaca gcatcaagac
     1861 gcaggcggaa gtggagcgtt ttaaagccca tctgcataaa cagctggaag atgagcggcg
     1921 gccggacctt aactatgtga ttgtcggtgc gggcccgacc ggtatcgaac tggctggcgc
     1981 gttgcctcat tatttgcaac acatcatggc taaccatggt atgccggcac gaaaagttca
     2041 catcgacctg atcgaggccg caccgcgcct gttaccacgc tcccccaaag acacctcacg
     2101 ctccgtccgg cgtcatctgc gcaagctcgg tgtaaggctc tacctcggca gcgttgtcca
     2161 gggagaaaca gctgacgagc tgaccgtcaa cggcaagcct atccgcagcc atacggtaat
     2221 ttggacagcc ggagtaacca accacccgtt ttttgtcgct aatcaatttg tacttactaa
     2281 gcggggcaag gtagctgtgg acacctatct gcaggccgag gatagcattt ttgtactcgg
     2341 cgataatgca aatacaccct atagcgggct ggctcaaacc gctctgtacg acggcaagtt
     2401 cgtagccaat aacttacggc gacgggcagt cgggaagcgc ttcaagagct acattgccaa
     2461 acaaccgatt tcagtcattc cagccggacc gcgctgggcg gccgtggagt ggggagtctt
     2521 gcatttccaa ggccggcttg gttggctgct gcgggaggcg gccgacttga tcggcttcca
     2581 cgatcttgaa tcctggccat tagccaccaa acagtggttg accgaattca gtggccagga
     2641 caactgtgct gtctgtaccg ccgcacaagt caattagcga acctcatccg gttccatata
     2701 caatataggt atggatgagg tgtttcgggc cgcgtacaag cagctgaaca acaagcagcg
     2761 cgaggctgtc gacacgatcg acgggccgct cttagtaatt gccggaccag gaaccggcaa
     2821 gacgcagctg ctcagtatgc gggtggccaa tattattcag caaacggaca ccgcgccgag
     2881 caatatactc tgcttgacat tcaccgacaa tgccgctcgt aacatgcgcg aacggcttga
     2941 aaccatcatc gggcaaccgg cttaccatgt agctattcat accttccata gctttggcgg
     3001 cgaaattatt agccagtatt ctgattactt tgccggacga caattgctgc agcaaatcga
     3061 cgatctcggt cgctacgagc tgctccgcga gatatttgag gcactgccgt atgataatcc
     3121 actcggtgtc aaggttggtg acgaatttgt gcatcttaga agcacgcttg acatcattag
     3181 ctggctaaaa cagaatgctc tgacaccagc cgagatgcgc acgctaattg ctgtgaaccg
     3241 gcaatttatg gaagtcatga ccgatgagtt agccgaaaca ttcgccgtca cgccctcacc
     3301 gaaacttttg tcgaaataca agaagtttgt gggtacggcg caaaaacata tcacaggtca
     3361 tcggcattat ggctttcctg aatatgctgc tgaatgtgtc ggcgagctgg aagccgcgat
     3421 ggcgaacacg agcgtcgatg gacggtatgc cccggcaata accgcctggc gcaacaagtg
     3481 gtgcgtcaaa gatgcccaaa accgtcacgc ctttaaagac gctgggcaaa acatgcgcaa
     3541 agtgcaggct gtcgccgacg tctacgagca gctgctggag cgcatgagcg agctaggttt
     3601 atatgatttt gatgacatga tcgtagaagt agtgcatgcc ctggaaacca agcctgacct
     3661 tcgcttcacg cttcaagaac ggtatcaata tttgctagtc gacgaattcc aagacaccaa
     3721 caaggcccaa ctccgaatgc tgctggcgct gggcaataat cccgtcaacg aagggcaacc
     3781 gaatattatg actgtgggcg atgacgacca agccatttac gcgtttcagg gagcggaagt
     3841 gtcaaatatg gtggcctttg ccgagcagta tcctaccgcc aagcggatta cgctggaaga
     3901 gaattaccgt tccaatgccc atatcttgga tgccagcttt gccgtggccg gccaaattac
     3961 cgatcgtgtg cagtcaatct tgcccgacac gcacaaaaca ctggttccgc gtgcaaacta
     4021 cggcacagat atcttgcagc acactgtttt cccaagcgag ctcgctcagt atgattggat
     4081 tgctgaccaa atcaaggcgt atctgaagca aggaatacag cctgaggcaa ttgcggttat
     4141 cgctcctcgt caccgctacc tggaacggct catgccatat ctcggcaatt gtcatatccc
     4201 agtggcttac gaacggcgcg aggatatcct ggaatcgccg attattgtac agctgctgac
     4261 catgacgcgc ctggtgactt ccattgccga taatcaacaa tccgaagtcg atcacctgat
     4321 aagccagata cttggctacg atttttgggg aatctccgct gatgacctcg tgaaaatcag
     4381 cttggaatgt tacaacaagc atgagcattg gctacccgta ctcttagctc atgagaacga
     4441 gaaacttcgc gacatcacca cttggtttac ctttctcgcc aagcaaagcg ctattgaacc
     4501 tttggaatac atgctggaca tactgctcgg aaaccttccc gacggaaaag ataatgaata
     4561 cgacgactta ttgctgcctg ttaatcgtaa gccctggttc atatcgccaa tgtttgaatt
     4621 ttatttcaat cctgagcgtt atgaactggc cactgatact tatttgatgc tccttgggca
     4681 gctct
//
LOCUS       DATNGP010000003         3801 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_4626914, whole genome shotgun sequence.
ACCESSION   DATNGP010000003 DATNGP010000000
VERSION     DATNGP010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3801
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4626914"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..631
                     /locus_tag="VN778_00065"
     CDS             <1..631
                     /locus_tag="VN778_00065"
                     /inference="COORDINATES: protein motif:HMM:NF014342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HXR49410.1"
                     /translation="VMINDHTRASAGELIYLLAKQLNWPLTVAAEEFLMTSILGDTQG
                     LSNQLASAETYRIMAEFVEHGVNRPQLEELRREYGKMQPTIYRYKGQLIQRTEISEAG
                     KIAHVTIPQSEINEYSPLYNPAPLIQNDMLQTTGVQVAIVFKQYDDGRITGAIRCNPT
                     AGIAAELAEQLGGGGHAFASGFKVTDGRSINEIRADCLAKAAGLLAKLG"
     gene            643..2046
                     /gene="cysS"
                     /locus_tag="VN778_00070"
     CDS             643..2046
                     /gene="cysS"
                     /locus_tag="VN778_00070"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008578738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HXR49411.1"
                     /translation="MKLYNTLSRQIEEIKSLNPPQITVYTCGPTVYDYAHVGHWFNYV
                     RMDTLIRALKASGLQPKWVMNITDVGHLTSDADEGEDKLEKGAQREGKTAWEIAAFYT
                     QDFLDGMQALNLLTPDFIVKATDHISDQIELIKRLEERGYTYVIDDGVYYDTSKFEGY
                     ASFAKLDLDEQQGGARVLLNPQKRNSSDFALWKFSLKDRKRDMEWDSPWGKGFPGWHI
                     ECSAMSMKHLGETLDIHTGGIDHIPVHHTNEIAQSEAATGKRFANYWLHSNHIMVDGE
                     KISKSLGNSIRLQEIASRGFSPEVLRLHILESHYRNQSTFSWESLEAASNRLQNLQAF
                     ADLRFQPIEAHEHETEELDFNQYRKLIGEAVLEDLNTPQALFQLNQLIDLRSNAILVS
                     EKDGLGAFLSFIDELLGTRLLDSDDLSDEQKSLVKAREEARRKQEWAKSDQLRDLLTE
                     QGIGLRDTPHGAIWFRS"
     gene            complement(2027..3145)
                     /locus_tag="VN778_00075"
     CDS             complement(2027..3145)
                     /locus_tag="VN778_00075"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HXR49412.1"
                     /translation="MLHFRKSEEVPEVALTVSYQTFFRLTLLVIATITVLAAIHKASH
                     ALLLIFAAFFLALALNAPVYWLSRQLPGKRRGSRSLATTLSFLIVIIFLGGFLAGIVP
                     PLVRQTNSLIHVAPSLVRDLRSQDSPTGKFIRKYHLEKQIGNFSNQLSARLKNAGGAA
                     FSTLQKIGSSIFSLVTVLVLTFMMLVEGPRWLAFARDVIPDRHHAVADRLARDMYRVI
                     RGFVNGQVTLAALAAVLIMPAVLLLHIGYPAALMVVIFVCGLIPMIGHTIGAIIITIV
                     ALFHSTSAAIIILAYYILYQQLENYLIQPRIQANSTNMSPLLVFMSVVLGVSFGGLFG
                     GLVAIPVAGCLRIAVLEYLRTRGIINTPRCEQVTSETK"
     gene            complement(3157..>3801)
                     /locus_tag="VN778_00080"
     CDS             complement(3157..>3801)
                     /locus_tag="VN778_00080"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HXR49413.1"
                     /translation="MDTEVLPNVIDYQHFHNAKPLPQYEDDKLTVLFLGRLVPRKGSL
                     LLLQAIAKLAIDPDLPPFRVLICGRGPLEGALRRFIAQHQLDPIVSMVGFVSEEEKPR
                     YYASADVSVFPSSGGESFGIVLLEAMAGGHAAILAGDNPGYHSVLALRSELLFNPHDS
                     NELADRIKLYLLDDTLRRQAGTWGAKYTPDFDVSVVGGQLLTRYYEVLRKRRKQ"
BASE COUNT         1052 a          929 c          954 g          866 t
ORIGIN      
        1 cgttatgatc aatgaccaca ccagggcatc agccggcgag ttgatttatt tgctagcaaa
       61 gcagctcaat tggccgctaa cagttgccgc ggaggagttt ttgatgacgt ccatactagg
      121 cgatactcaa ggcctctcca accagctggc ctcggccgaa acataccgca ttatggctga
      181 atttgtcgag cacggggtca accggcccca gcttgaagaa ttgcgccgcg aatacggcaa
      241 aatgcagccc acaatttacc gctacaaagg ccaactgata cagcggaccg aaatttcaga
      301 agctggcaag atcgcgcacg ttaccattcc gcaatctgaa attaatgagt atagtccgct
      361 ttataacccc gcacccctca tccaaaatga catgttgcaa actaccggcg tacaagtcgc
      421 gatcgttttt aagcaatatg atgatgggag aattaccggg gctattcgtt gcaaccccac
      481 tgccggcata gcggccgagc tcgccgaaca gctcggtggc ggtggccatg cttttgccag
      541 tggtttcaaa gtaacagatg gccggtcaat aaacgaaata agggccgatt gtctggccaa
      601 agctgctggt ttacttgcta aacttgggta gaggaaacat caatgaagct atataacact
      661 ctctctcgac aaattgaaga aattaagtcg ttgaatcctc cccagataac cgtctataca
      721 tgcggcccga cggtttacga ctacgctcat gtcgggcact ggttcaatta tgttcgcatg
      781 gatacgctta ttcgcgccct caaagctagc ggcttacagc caaaatgggt tatgaacatc
      841 acggatgtcg gccatttaac gagcgatgct gacgaaggag aagacaagct cgaaaaaggc
      901 gcacagcgag agggcaaaac agcctgggaa atcgctgctt tctacaccca agatttttta
      961 gacggaatgc aggcccttaa cctgttaacc ccagacttca ttgttaaggc taccgatcat
     1021 atttcagatc aaatcgagct tataaaaagg cttgaggaaa gaggctacac ctacgtcatt
     1081 gatgacggtg tgtactatga caccagcaag tttgaaggct acgctagctt tgctaaactt
     1141 gaccttgacg agcagcaagg cggagcgcgc gtgttgctca atccgcaaaa gcgtaattcc
     1201 tcagactttg cactttggaa gttttcgttg aaagaccgta agcgagatat ggaatgggat
     1261 agcccttggg gtaaaggctt tcctggttgg cacattgagt gctcggcaat gagcatgaag
     1321 caccttggcg aaaccttgga cattcatacc ggtggcattg accatattcc cgtccaccac
     1381 accaatgaaa ttgcccaaag tgaagcggcg accgggaagc ggttcgctaa ttattggctg
     1441 cacagcaatc atattatggt ggacggtgaa aaaatctcga aatcacttgg caatagcatt
     1501 cgtttgcaag agatagcttc gcgcggcttt tcgcccgaag ttctgcgatt acatatttta
     1561 gaatctcatt accgcaatca atccactttt agctgggaaa gcttagaagc agctagcaat
     1621 cgcctccaga atctgcaggc ctttgctgat ttacgctttc aacctattga agctcatgag
     1681 catgaaacag aggagctcga tttcaatcag tatcgaaagc tcattggaga agccgtctta
     1741 gaggatctca atacacctca agccctgttc caacttaacc agctcattga tctgcgcagt
     1801 aatgctatac tcgtgagtga aaaagatggc ttaggcgctt tcttgagttt tatagatgag
     1861 ttactaggca ccaggctttt ggactccgac gaccttagtg atgaacaaaa aagcttggtg
     1921 aaggctcgtg aagaagcgcg tcgtaagcag gagtgggcga agtctgatca attgcgagat
     1981 ctgctaacag agcaaggcat cgggctgcgg gacacgccac atggtgctat ttggtttcgc
     2041 tcgtaacttg ctcgcagcga ggcgtgttaa tgataccgcg tgtcctaaga tactctagaa
     2101 cggcaatgcg caaacagccg gcgaccggga tggccactaa gccgccaaag agaccaccaa
     2161 aactcacccc taaaacgacc gacataaaaa ccaaaagagg agacatatta gttgaattgg
     2221 cttgaatacg cggctggatc aggtagtttt ctagctgctg gtataggatg tagtacgcca
     2281 agataatgat ggcagcagag gtagagtgga agagcgcgac aatggtaatg ataatagcac
     2341 cgatagtatg cccaatcatc ggtataagcc cgcagacaaa aataacaacc attagggccg
     2401 ccgggtagcc gatatggagc aacaaaactg ccggcatgat gagtaccgcc gccagtgccg
     2461 ctagcgttac ctgaccattt acaaagcctc ggattacacg atacatgtca cgcgccagcc
     2521 gatcagccac cgcgtggtgc ctatcgggaa tgacatcccg ggcaaaggcc agccaacggg
     2581 gcccctccac gagcatcata aaggttaaca ccaacaccgt taccagcgag aatatactgc
     2641 tgccaatttt ctgcagtgta gaaaacgccg cacccccggc attctttaag cgggccgaca
     2701 gctggtttga gaaattgccg atttgcttct ccaagtgata ctttcgtata aatttgccag
     2761 tggggctatc ttggctgcgc aaatcccgaa ccaaactcgg tgccacatga attaagctgt
     2821 tggtttgacg gacaagtggc ggtacgatgc ctgccaaaaa cccgccgagg aagatgataa
     2881 ctattaaaaa ggagagtgtt gtcgctagtg agcgactgcc acgtcttttg cccggcagct
     2941 gcctgctgag ccaatatacg ggggcattaa gtgccaacgc caagaagaag gcggcaaaga
     3001 tgagcaagag ggcatgcgag gctttgtgaa tagctgccag taccgtgatg gtagcaatta
     3061 ccaggagcgt taatctgaaa aaggtttgat aagagacggt cagggcgact tcgggcactt
     3121 cttcgctctt tcggaaatgt aacatagctt gattatctat tgctttcggc gtttacgcaa
     3181 gacttcgtaa tagcgtgtta aaagctggcc acctacgaca ctgacgtcaa agtctggggt
     3241 gtatttggcc ccccaggttc cagcttggcg gcgcaaggta tcatctaata gatatagctt
     3301 tatcctgtcg gccagctcat tagaatcatg tggattaaaa agcagttcag aacgcagagc
     3361 gagcactgaa tgataccccg gattatcgcc tgctaaaata gctgcgtggc cgccggccat
     3421 ggcttcgagc aatacaatgc cgaagctttc accaccactg ctgggaaaaa cagatacgtc
     3481 cgctgaagca tagtaacgcg gtttctcttc ttcgctgaca aagcccacca tggatacgat
     3541 aggatccaac tgatgctgcg ctataaaacg ccgcagtgca ccttcgagcg gtccgcggcc
     3601 gcagataagt acgcggaacg gtggcagatc aggatcaata gcaagtttgg cgattgcttg
     3661 cagcagcagt aagctgcctt tacgcggtac taatcttccc agaaacagaa cggtaagctt
     3721 atcatcctca tactggggca acggttttgc gttatgaaaa tgctgatagt caataacgtt
     3781 cggtaacacc tccgtgtcca t
//
LOCUS       DATNGP010000004         4641 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_1836498, whole genome shotgun sequence.
ACCESSION   DATNGP010000004 DATNGP010000000
VERSION     DATNGP010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4641
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1836498"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1084
                     /locus_tag="VN778_00085"
     CDS             <1..1084
                     /locus_tag="VN778_00085"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXR49414.1"
                     /translation="IGDGAIATDEFGRITRVNPVALKLLQYTESELLGEWFPKIVVAM
                     ELNGKVLNLIDRPITRAFLTGQPVAAKLFYRRKDGQPLPVSINVSPILLNGKPLGAIE
                     LFRDITVEEEIDRMKSEFISLASHQLRTPLSAIKTYSHMLVDGYMGKITPTQKKALHT
                     IISATNRMNELISTLLNITRIESGSIAATPKVLKVDRVVEEVLKELALMAADKSIATS
                     LKVSGKGSTSIKTDNLILKEILTNLISNGIKYTPEGGRIDVTVKPRLADVLISITDTG
                     WGIPKDVQNQIFSKFFRAHNIVRRETTGTGLGLYLVRGLIDILGGRIWFKSAEQAGTT
                     FTFVLPRNKVFDETKESKAGLGAIKG"
     gene            1186..1578
                     /locus_tag="VN778_00090"
     CDS             1186..1578
                     /locus_tag="VN778_00090"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXR49415.1"
                     /translation="MARILVVEDDKDLNNAYCLILNSEGHKVETAFNGQEALKKVEKF
                     KPDLILLDLLMPIMGGLEFLQRYDLTKIHPKVKVLIFTNMENSPEVTVAYKLGAHRII
                     IKSWTAPHNLARVIEEALKSKPAAKTTA"
     gene            complement(1568..2257)
                     /locus_tag="VN778_00095"
     CDS             complement(1568..2257)
                     /locus_tag="VN778_00095"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HXR49416.1"
                     /translation="MAVTTVNDQLQAFLERWPTKTFRKGEIIIFQGEAPRGAFIVRKG
                     TVKAYNLSVGGDEKPVAFYSEGAVFPGSWVYRKMASAIYYYEAFTPEVSLYLVNREAY
                     VDHVKSRPQLLYQELERYLTDQLGMSMRLNALQHSRASDKLLYTLHYLALSHGVTIAP
                     QQIEITLDLTHQDFANLTGLTRETAATELNKLKRQKVITYGKHTPYQLHLGRLMQLLN
                     DQYISDLQIKL"
     gene            complement(2321..3883)
                     /locus_tag="VN778_00100"
     CDS             complement(2321..3883)
                     /locus_tag="VN778_00100"
                     /inference="COORDINATES: protein motif:HMM:NF015755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HXR49417.1"
                     /translation="MPQNNNYRPSPPRPGASSRARPGQDIVVVKKQASQFPTVGMTLP
                     SYHYDPRDRHRRTAGSPVVKRRWWRRIFNRTVFKRAFITLVILVLLVGGWVGGKFLYN
                     AHKLFGGSILGALRTTKLKGESTGRVNILLAGNSADDVGHNGGDLTDSIMIVSIDTRN
                     NTAFLLSVPRDLWVYIPGGGYQKINAAYVVGQGDKFSEPGYPGGGMGLLEQIVSQNLG
                     LPIDYYALIDYSALKEAVNVVGGIQVNVKSSDPRGLYDPSIDYATHGPLVKLSNGVHT
                     LNGEQALDLARARGDAYGSYGFGGSDFERTQNQRMMLVAIKDKSTSLGVLTNPAKLSS
                     LSDAVGNNVKTDMKLSEVHRLVDLVKQIGSSSIQSLSLNSANGKNLLTSYESRDGESA
                     LIPAAGIDDFTAIQAFIAQQTSSNPVLRENANVVLLNGTEVSGLASQERTNLRVKNIT
                     VTAIGDAQTSTQTVTSIIDASNGKKSATKQLLTQLFGNNVTTTNSYANMYPDADFIVV
                     LGTDQSSHGTNN"
     gene            4147..>4641
                     /locus_tag="VN778_00105"
     CDS             4147..>4641
                     /locus_tag="VN778_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008358307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HXR49418.1"
                     /translation="MKLVVQIPCFNEEATLPLVLESIPKKVAGVDEVIILIIDDGSTD
                     KTVEVAKSYGVKHIVHHVRNQGLGRSFHDGVLMALELGADIVVNTDGDNQYPQGRIDD
                     LVQPILKSEADIVIADRQTHKIEHFSSTKKFLQRVGSAVVSKAAGIQLPDAASGFRAY
                     SRESL"
BASE COUNT         1220 a         1122 c         1137 g         1162 t
ORIGIN      
        1 tattggtgac ggagcaatcg ccactgacga gttcggcaga ataacccgcg ttaacccggt
       61 agctttaaag ctgcttcagt acaccgagtc agaattgctc ggcgaatggt tcccgaaaat
      121 cgtggtcgcc atggaactta atggcaaggt gctcaatctc atcgaccgcc caatcaccag
      181 agctttccta acgggccaac cggttgctgc taagcttttc taccgccgta aagatggtca
      241 gccattgcca gtgagcatca atgtttcgcc tattttattg aacggcaaac cgctcggcgc
      301 catagaattg tttcgtgata ttactgtcga ggaagaaatc gacagaatga aaagcgaatt
      361 tatttcacta gcttcacacc aactccgtac accgctatcg gccatcaaga cctactccca
      421 catgctcgtt gacggctaca tgggaaagat aacaccaaca cagaaaaaag cattgcatac
      481 tattatttcg gctactaacc gtatgaatga gcttattagc acgctcctaa atattactcg
      541 tattgaaagc ggtagtattg ccgccacgcc gaaagtactc aaagttgatc gggtagttga
      601 agaagtactt aaggagctcg cgctgatggc agccgacaag tccatcgcaa cttcgttgaa
      661 agtcagcggc aagggcagca cttctataaa aaccgataac ctcatcctta aagaaattct
      721 taccaatctt atatcgaacg gcatcaagta tacgcctgag ggcggccgca tagacgtcac
      781 tgtcaagcct cgcttagccg acgtcctcat cagcattacc gatacgggct ggggtattcc
      841 gaaagacgtc cagaatcaga tcttcagtaa atttttccgt gcccataaca ttgtacgccg
      901 agaaacgacg ggcaccgggc tgggacttta cttggttagg ggtttgatcg atattttagg
      961 cggccgtatc tggtttaaga gtgccgaaca agccggtacc acctttactt ttgtcctccc
     1021 acggaataag gtttttgatg agacgaaaga atccaaagca ggacttgggg caattaaagg
     1081 gtagtagaca gcttgtctct tatgtaagaa attcctcacg ccttgtaccg cttatgtggt
     1141 ataaataatc tataattcta ttcatcaaga agggaagcac acccgatggc acgtatctta
     1201 gtagtagaag atgacaaaga tctcaacaat gcgtattgcc tcatcctgaa cagcgaaggt
     1261 cataaagtgg agaccgcttt caacggccag gaggccttaa aaaaagtcga aaaatttaaa
     1321 ccagatctta tattgcttga tttgctgatg ccgattatgg gtggcttgga atttttgcag
     1381 cgttatgatt tgacgaaaat ccatcccaaa gttaaagtac tgatctttac aaatatggaa
     1441 aattcaccag aagtaacggt ggcgtataaa ctcggcgctc atcgcattat catcaagtcg
     1501 tggacagccc cgcacaatct cgcccgggtc atcgaagagg ccctgaagtc taagccggca
     1561 gccaagacta cagcttgatc tgcaagtcag aaatatattg atcatttagg agttgcatca
     1621 gcctaccgag atgcagctga tacggcgtat gcttgccata ggtgataact ttttgacgct
     1681 tcagcttatt taattctgtc gcggctgttt cacgagtcaa gcccgttaga ttggcgaagt
     1741 cttgatgcgt tagatccaat gtaatctcta tttgttgcgg cgcaatggtc acgccatgac
     1801 tgagcgctag gtagtgcaag gtgtagagca gcttgtcgct ggcccggctg tgctgaagcg
     1861 cattcaggcg catcgacatg cccaattggt ccgtcagata gcgttctagc tcctgataga
     1921 gtaattgtgg gcggcttttg acgtggtcca cgtacgcttc gcgattaact aagtacaagc
     1981 tcacttccgg cgtaaaagct tcgtaataat atatggcact cgccatcttc ctatataccc
     2041 aagagcccgg gaaaacggca ccttcagaat aaaaggcaac gggcttttcg tcgccaccaa
     2101 cgctaaggtt gtaagcttta acggtgccct ttctaacgat gaaggcgcct cgcggggctt
     2161 cgccctggaa tataataatc tcacctttac gaaaggtttt ggtcggccaa cgctccaaaa
     2221 aagcctgtaa ctggtcattg acagttgtga cagccatcgt ctagttatag tacaccgggt
     2281 gactggccta taacaagttg cttaggcgcg taacagaggt ttagttgttg gtgccgtgtg
     2341 aagactggtc cgttcccagt accacaataa agtcggcgtc aggatacata ttagcatagg
     2401 agttggtggt ggttacatta ttgccgaaga gctgtgtaag gagctgcttg gtagccgatt
     2461 ttttaccgtt agaagcatcg atgatggacg tgacagtctg ggtagaagtt tgggcatcac
     2521 caatagctgt aaccgttatg tttttaactc gcagattggt acgctcctgc gatgccagcc
     2581 cactcacctc agttccgtta agtagcacga catttgcatt ctcacgcaag acgggattcg
     2641 aggacgtttg ctgagctatg aaagcctgga tagcggtaaa gtcatcaatg ccagcagctg
     2701 gaatgagcgc cgattcaccg tctcgagatt catagctagt caacagattt ttaccgttag
     2761 cactatttag actcagggac tggatgctgc tcgagccaat ttgcttaacc agatcgacca
     2821 ggcgatgtac ctcacttaat ttcatgtcag tctttacgtt attgccgaca gcatcagata
     2881 aactcgaaag tttggcaggg ttcgtcagaa caccgagcga ggttgattta tcttttatgg
     2941 ctaccagcat catgcgctga ttttgcgtcc gctcaaaatc gctaccgcca aagccgtacg
     3001 agccgtaagc gtcgccgcgg gcacgcgcca gatcaagtgc ttgctcgcca ttgagtgtat
     3061 gaaccccgtt acttagcttg accaacgggc catgggtggc gtaatcgata ctcggatcat
     3121 acaagccacg cggatcactg ctcttaacat tcacctgtat gccgcccacg acgttaacag
     3181 cttctttgag agcgctgtaa tcgatgagtg catagtaatc aattggcaag ccgagatttt
     3241 ggctgacgat ttgttccagc aaacccatgc cgcccccagg atagcccggt tcgctgaact
     3301 tgtcgccctg gccaacgacg taagccgcat tgattttctg ataaccgcct ccgggaatat
     3361 agacccataa atctcttggc acgctcaaca aaaaggcggt gttattgcgc gtatcgatgc
     3421 tgacaatcat aatcgagtcc gttaaatcgc caccgttgtg gcccacgtca tcggccgaat
     3481 tgccggctaa taaaatatta actcgccccg tgctttcacc cttgagtttg gtggttctca
     3541 gggcacctaa tatgctaccc ccaaacaact tatgggcgtt gtacagaaat ttaccgccga
     3601 cccagccgcc aaccagcaat acaagaatca cgagcgtaat aaaggctctt ttaaagactg
     3661 tccggttaaa tatgcgtcgc caccaccgtc ttttgacgac cgggctacca gctgttcgcc
     3721 gatgacgatc acgcggatcg taatgatacg agggtagcgt catgccgact gtcgggaact
     3781 gcgaagcctg ctttttcacg accacaatat cctggccggg ccgcgctcga gagctggcac
     3841 ccggtcttgg tggggaggga cgatagttat tgttctgggg cattgagagt tactgtccat
     3901 tataccgatt tgggacactc cttgctacgt tgatttgaca gtaggctagt gggctgaaat
     3961 tcggctgagg accgccgctc agacctctga acaaactaca gtcggcttaa gctaatcctc
     4021 agcgtggcag tatgtaatca agccaaagta gcagcgaaga tagtaattta agacagcaga
     4081 aatgactaaa gggtataact gattggctgc tgctgcgcta tactagaaac gaaagttgtg
     4141 aggcttatga aattagttgt ccaaatccct tgcttcaatg aagaagcgac actgccactg
     4201 gtactggaaa gtatccctaa aaaagtggcc ggggttgatg aggttatcat cttgattatt
     4261 gatgatggct caactgacaa gaccgttgaa gtggccaaaa gctatggcgt caagcacatt
     4321 gtgcatcacg tacgtaatca ggggctaggc cgttcatttc atgatggcgt gttgatggcc
     4381 ttggagctag gtgccgacat tgtcgtgaac accgatggcg ataatcaata tcctcagggg
     4441 cgcattgacg acctggtaca gccgattttg aagagcgagg cggacatcgt gatcgctgat
     4501 cgtcaaacgc acaagatcga gcacttctcg tctaccaaaa aattcctcca acgcgtggga
     4561 agtgcagtgg ttagtaaagc agctggtatt cagctgcccg acgccgccag tggcttcaga
     4621 gcatacagcc gggaaagctt g
//
LOCUS       DATNGP010000005         2024 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_601897, whole genome shotgun sequence.
ACCESSION   DATNGP010000005 DATNGP010000000
VERSION     DATNGP010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2024
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_601897"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1057
                     /gene="argS"
                     /locus_tag="VN778_00110"
     CDS             <1..1057
                     /gene="argS"
                     /locus_tag="VN778_00110"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HXR49419.1"
                     /translation="FAQIYWTCRQWSYDYFAELYRQLQVTPFDRFIPESEVTPLGVQT
                     VTEQLEKGVFTRSDGAIVFPGEDFGLHTRVFINSHGLPTYETKDIGLSLTKWRDYHFD
                     ESIIITANEQEQYMQVVVAALKQFEPEPAQRTRHLTHGIVKLQGGVKMSSRRGNVLTA
                     IDLLDAARAAGAETGTNPSEETILAAVKYAFTKNRIGGDIIYDPKESIALEGNSGPYL
                     QYAHARARSILGKALAARHPRKNGDPVESSRSSFNWIPDQVGNDGILLEPAERSLVRK
                     ITEYTEVVEKATNELLPHYICTYLYELAQAFNSFYEHNRVIGDPREVARLTLVAHYAD
                     TLKNGLELLGITAPDKM"
     gene            1061..1528
                     /locus_tag="VN778_00115"
     CDS             1061..1528
                     /locus_tag="VN778_00115"
                     /inference="COORDINATES: protein motif:HMM:NF013869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MscL family protein"
                     /protein_id="HXR49420.1"
                     /translation="MTDTPTPSEQKPLKEKSEAELKREAKARLRLLSPATIKAIVREV
                     LHNRVGGSMSGFVTFLRERAIVGLAVGFAIGSQAQVVVKQFISSFVDPLFKLLIPGDQ
                     VLSARNWTIGFGGHTAIFGWGAMVYTLIDFLFILFMIYLVIHLLRLDQLDKKQ"
     gene            complement(1531..1872)
                     /locus_tag="VN778_00120"
     CDS             complement(1531..1872)
                     /locus_tag="VN778_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013157287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXR49421.1"
                     /translation="MKSLLTKQPRLLEPMTGCIEAAADILGSKWTALILRDIASGHCR
                     FCALEKTVGKINPRTLSKRLDDLELQGIITKVTYPEVPPRVEYRLTQKGAGLLPILEQ
                     MAAWGEKYPGA"
BASE COUNT          506 a          472 c          567 g          479 t
ORIGIN      
        1 atttgcacaa atctattgga cctgccggca gtggagctat gactattttg cagagctgta
       61 tcgccagttg caggtaacgc cttttgaccg ctttattcct gaaagcgagg tgacaccgct
      121 gggcgtacaa acggtaacgg agcagctgga aaaaggcgtg ttcacgcgta gcgatggcgc
      181 catcgtcttt cccggcgagg atttcggcct gcacacacga gtattcatta actcgcatgg
      241 tctgcccacg tacgaaacaa aagatatcgg cttaagcctg accaaatggc gtgattatca
      301 ttttgatgag tcgatcatca tcactgctaa cgaacaggag cagtacatgc aggtggtcgt
      361 tgcggcgctt aaacagtttg agcctgaacc cgcgcagcgg acgcgtcatt tgacgcatgg
      421 tatcgtcaag ctgcagggag gggtgaagat gagttctcgc cgcggtaatg tcctgacggc
      481 gatcgatttg ttagatgccg ctcgtgctgc cggcgccgaa acgggtacaa atccgtccga
      541 ggagacgatt ctggcggccg tgaagtatgc atttaccaaa aaccgtatag gcggtgacat
      601 tatttatgac cccaaagaat caattgcgct cgagggcaac agcgggccgt acttgcagta
      661 cgcacatgcg cgcgcacgat cgattttggg aaaggctcta gcggcccgtc atccccgtaa
      721 aaatggggat ccagtagaaa gtagcagatc ctcatttaac tggattcccg atcaggtcgg
      781 gaatgacgga atacttttgg aaccagccga gcgaagttta gtacgaaaaa ttaccgaata
      841 tacagaggtg gttgagaaag caacgaacga actgctaccg cactatatct gcacgtattt
      901 gtacgagcta gcgcaagcat ttaatagctt ctacgaacac aaccgggtga taggcgatcc
      961 gcgtgaagta gcgcggctga cgctggtagc gcactatgct gatacgttga aaaacggatt
     1021 ggagctattg ggtatcaccg cacctgataa aatgtaaact atgacagata cacctactcc
     1081 gtccgaacaa aaaccgttga aggaaaaatc cgaagctgag ctgaaacgtg aagccaaagc
     1141 ccgactgcgc ctgctgagcc cagcaaccat taaagcaatt gtaagagaag tgctccacaa
     1201 cagagttggc ggctcaatgt ccgggtttgt gacgttcctc cgcgagcgag caatcgttgg
     1261 tttggcggtt ggttttgcaa tcggctcgca agcccaggtc gtggtgaaac agtttatttc
     1321 gagcttcgtc gatccgttat ttaagctgct gatcccgggc gaccaagtat tgagtgcgcg
     1381 caactggacg attggcttcg ggggccacac cgccatattt ggctggggtg caatggttta
     1441 tacgctaatt gattttctgt tcattctatt catgatctat ctggtgatcc acttgctgcg
     1501 cctagaccag ctcgataaaa aacaataggc ctaagcgcct gggtattttt caccccaggc
     1561 ggccatttgc tcgagaatcg gtaatagacc agcgcctttt tgggtgagcc ggtattcgac
     1621 gcggggcgga acttcggggt aggtaacttt agtaatgatg ccttgtaact ccaggtcatc
     1681 gaggcgcttg gacagggtgc ggggattaat cttgccgacg gttttttcca gggcgcaaaa
     1741 gcggcagtgg ccactagcaa tatcgcgcaa aataagtgcc gtccacttgc tgccgaggat
     1801 gtcggcggcg gcttcaatgc aacccgtcat cggttccaat agtctcggtt gttttgtaag
     1861 gagggatttc atactttaca aagtatagtg atggcgttat gctgataata tacaaagtat
     1921 accataggag gatagcggat gaaactggcg gttattgtag gcacggtgcg tgacggtcgt
     1981 ctgagtgaac gtttggcgaa atgggtagcc gccagcgcgg ctga
//
LOCUS       DATNGP010000006         3161 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_1829727, whole genome shotgun sequence.
ACCESSION   DATNGP010000006 DATNGP010000000
VERSION     DATNGP010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3161
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1829727"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..676)
                     /locus_tag="VN778_00125"
     CDS             complement(<1..676)
                     /locus_tag="VN778_00125"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXR49422.1"
                     /translation="MADRQLKVAIVCDWLVGIGGAERVVLELHKLYPEAPIYTSQYDP
                     GRIDWFPDADVRTTWLQHLPKSLKKFLPLLRAWAFSRLDLSDYDLVISSSGAEAKGVK
                     TGPNTVHISYCHSPTHYYWIRRDEYLQNPGFPRGLNWLARLGLRLLLEPLKHWDRQAA
                     KRPNFMIANSTHTQAMIKRYYRRDSTVIFPPVETNRFHVNGNVKPPLRHGFVIAGRQT
                     PYKRFDL"
     gene            complement(676..2115)
                     /locus_tag="VN778_00130"
     CDS             complement(676..2115)
                     /locus_tag="VN778_00130"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HXR49423.1"
                     /translation="MKNNASLVYNVALVVGDALALTVAFTVAYILRVTLNHRPLSASV
                     HAHTYIAILTSLLPFWILIFALLGLYNARIHEKRFSELGRLVIGTFIGMLFVISYSYM
                     TNTVIFPARLVTVYGFGLAFVFVLLFRTVARGVRRELFSYGVGINNVLIVGDTPITSE
                     LITALADTAVTGYRVIGVIGGAKHVLKITANMPIFKSFSEALEALNTKHLHTIVQTEL
                     YAGAPRNDEILTFAQENHVAYRFVPGNSELFVGNIEVDLFQAVPVIAVHQTALIGWGR
                     VVKRLSDLAFGGLMLIVASPFIVLIALAEMFSGSGSIFFRQVRLTRFNQKFTVFKFRT
                     QYAKYDGTTPEEAFEQMGKPELAKKYRNNGDYLSNDPRITPLGRFLRRTSLDELPQLF
                     NVMRGDISLVGPRALIPEELELYAKRHAILSVKSGLTGLAQVSGRRYISFDERRKLDL
                     FYVQNWSFWNDLVILAKTVWIVLFHKGAV"
     gene            2171..>3161
                     /locus_tag="VN778_00135"
     CDS             2171..>3161
                     /locus_tag="VN778_00135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49424.1"
                     /translation="MKTSALKYAILSWSTTLTMVIMLLMPFHAFLTVWGASAIGHYTA
                     WRLWKEALLLLCGFGVLFLLITDHKIRSHTLTRRLVQVTLLYAFVNLIWGLIAYHQHA
                     VTAKALGYGLIVNLRFLAFFLVTWASALRLNRLRIHWQWLVLWPAIIVVAFGLLQILI
                     LPHNFLSHFGYGPNTIPAYETINHNSHYIRIASTLRGANPLGAYLLIPISLFTILLLR
                     GKRLGLYSGLLAATLVVEFFSFSRSAWIGAALAVVTVLALSLRTDRARRIFAIIAAGA
                     VVVIGILTIALHNNVTFENYFLHTQHNSAVKTTSDQGHAAALKNGLHDLVHEPQ"
BASE COUNT          718 a          852 c          865 g          726 t
ORIGIN      
        1 ccagatcgaa tcgtttatac ggcgtctgcc ggccggcgat cacgaaacca tggcgcaagg
       61 gcggcttcac attgccgttg acgtgaaagc ggttcgtctc gactggcggg aaaatgacag
      121 ttgaatcacg gcgatagtaa cgcttgatca tagcttgagt atgggtcgag ttggcaatca
      181 tgaagttggg gcgcttggcg gcttgcctat cccaatgttt gagcggttcg agcagcaacc
      241 tcaggccgag ccgggccagc cagttcaggc cgcgcggaaa gccggggttc tgcaggtatt
      301 cgtcgcggcg aatccagtag tagtgggtcg gggagtggca gtagctgatg tgtacggtgt
      361 tcgggccggt tttgacacct ttagcctcgg cgccgctcga cgaaataaca agatcataat
      421 cactgaggtc gagccggctg aaggcccagg cgcgcagcag tggcaggaat ttcttcaggc
      481 ttttcggcaa gtgttgcagc caggtggtgc gcacgtcggc atccggaaac cagtcaatgc
      541 gaccaggatc atactgggag gtgtagatcg gtgcttccgg gtataatttg tgcaattcga
      601 gcaccacgcg ctcagccccg ccgataccaa cgagccaatc gcaaacaatc gctaccttga
      661 gttgtcgatc ggccactaca ctgccccttt gtgaaacagc acaatccaaa cagttttggc
      721 gaggatgact aggtcattcc aaaaactcca gttttgcaca taaaacaggt cgagtttgcg
      781 ccgttcgtcg aagctgatgt aacgccggcc cgagacttgc gctaggcccg tcagaccgga
      841 tttaacgctc aggatagcgt ggcgcttagc gtacagttcc agttcttcgg gaatcagcgc
      901 ccgcgggccg accaggctga tatcgccgcg cataacattg aaaagctgtg gtagctcatc
      961 gaggctggta cgtctgagaa aacgaccgag tggcgtaatg cgcggatcat ttgacaggta
     1021 gtcaccgttg ttccggtact ttttggccag ctccggttta cccatttgct cgaaagcttc
     1081 ttcgggggtg gtgccgtcgt acttagcgta ttgcgtgcgg aatttaaaaa cggtgaactt
     1141 ttggttgaac cgcgtcaagc gaacctggcg gaagaaaatc gaaccgctac cgctgaacat
     1201 ttcagcgagg gcgatgagca cgatgaacgg cgaagcaacg atgagcatca agccgccaaa
     1261 cgccaaatcc gacaagcgtt taaccaccct tccccagccg atcagcgccg tctgatgtac
     1321 agcgataacc ggcaccgcct ggaaaaggtc aacttcgata ttgccgacaa atagctcact
     1381 gttgcccggc acgaaacgat aggcgacgtg gttttcttga gcgaaggtca gaatctcatc
     1441 gttgcggggc gcgccggcgt acaattccgt ttgcacgatg gtgtgcagat gtttggtatt
     1501 gagcgcctcc agggcctcac tgaaactttt gaaaatgggc atattggcgg ttattttcag
     1561 cacgtgcttg gcgccgccga tcacgccgat cacccggtaa ccggtgacag ctgtgtcagc
     1621 cagagccgta atcaattcgc tggttatggg ggtgtcgccc acgatcaaga cgttgttgat
     1681 gcccacgccg tagctaaaga gctcgcgccg aacgccccgg gccacggtcc taaacagcaa
     1741 gacaaagacg aaggccagcc caaaaccata caccgtcact aacctggccg ggaaaattac
     1801 ggtgttagtc atgtagctgt aactgataac gaatagcatg ccgatgaacg tcccgatcac
     1861 caaccggcca agctcgctga agcgcttttc atggatgcgc gcattgtaga ggccgagcag
     1921 agcgaaaatc aggatccaga aaggcagcag gctcgttaaa atcgcgatgt aggtatgggc
     1981 gtggacgctg gcgctcaatg gtcgatgatt cagcgttacg cgcaggatat aggcaaccgt
     2041 aaaggcaact gttaaagcca aagcgtcgcc gaccaccaga gctacgttat aaaccaggct
     2101 agcgttattc ttcataaagt cgctataccc atgatacatc gcgaacgcgg tatactaaag
     2161 ggaagtcctt atgaaaacat cggcgctgaa atacgcgatt ttgtcttggt cgaccacttt
     2221 gaccatggta attatgttgc tcatgccctt ccacgccttc ctgacggtct ggggcgccag
     2281 tgccatcggc cactacacgg cctggaggct ttggaaagaa gccttgctgc tactctgtgg
     2341 cttcggcgta ttgtttttac tcattaccga tcacaaaatt cgctcgcata ccctaacccg
     2401 ccgtttagtg caggttacct tactctacgc gttcgttaat ctaatctggg gacttattgc
     2461 ttatcaccag catgcggtta cggccaaggc gctcggctac gggctcatcg tgaacctaag
     2521 gttcctggct tttttcctgg tcacctgggc cagcgcgctg cgcctgaacc ggttgcgcat
     2581 ccactggcag tggcttgtgc tctggccggc aatcattgtc gtagctttcg ggctgctgca
     2641 aatcctcatc ctgccgcata attttctgtc gcatttcggc tatggcccga acaccatacc
     2701 ggcgtatgaa acgattaacc acaactcgca ctatatccgc attgcttcga cgctacgcgg
     2761 ggcgaatccg cttggtgcgt atctgttaat accgattagc ctattcacta tcctgctact
     2821 ccgaggcaag cgcctcggac tgtacagcgg cttgttggcg gcgacgctcg tggtggaatt
     2881 tttcagcttc tcgcgcagtg cctggatcgg tgccgctctg gctgttgtca cggtgctggc
     2941 gctctctttg cgcactgata gagcgcgtcg aatttttgcg atcatagctg ctggcgcagt
     3001 cgtagtgatt ggaattttaa ccatcgcact gcacaacaac gttacctttg agaattactt
     3061 ccttcacacc caacataact cggcagttaa aacgacgtcc gatcaaggtc atgccgctgc
     3121 tttgaaaaat ggtcttcacg acctagtaca cgagccgcaa g
//
LOCUS       DATNGP010000007         3354 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_4612534, whole genome shotgun sequence.
ACCESSION   DATNGP010000007 DATNGP010000000
VERSION     DATNGP010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3354
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4612534"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(262..1401)
                     /locus_tag="VN778_00140"
     CDS             complement(262..1401)
                     /locus_tag="VN778_00140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49425.1"
                     /translation="MTKWLRPIGLASLVIVLVMGLSLQAIFSSRASADQITVRSLTLE
                     AGATDGGSKPGGVVKHLFAFTLPSVGATNVGSIKFLYCTAAGVMSDPCTMPTGLVTTS
                     ATLSNQTGATGFTMVNTTNGAPYLTRTAASVPAGTAVTYELSTVTNPTTTNKTFYVRI
                     ATYASIDTTGAITDSGTVAASTATQIVLTGTMPESLIFCAGATVNTTSGVPDCSTATP
                     GAITFNQLFSPTDTATATSQMAASTNAGSGYSITVNGPTLTSGSNTVTAMAAAAAGVR
                     GTSQFGLNLRANTVATSTPAVGTDVAPAAGAGGGTYKGEPATGYGTVDQFKFVSTNSV
                     ADSAFGGAGGTDAQIFTVSYIVNVPGSQPAGTYTTTLTYICTPTF"
     gene            complement(1526..2251)
                     /locus_tag="VN778_00145"
     CDS             complement(1526..2251)
                     /locus_tag="VN778_00145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49426.1"
                     /translation="MRRLLGLATTLLLIMLGMAVPAYADNNELQSTHFRFDQSVVGGS
                     GLDQENSSSYQLGELIGDLAVGSSSSTNFQLNSGFDTTGEPALTFIVDSSGVNFGSFS
                     AAAATVATSTFEVIDYTSYGYIVQIYGNPPTNGSHTITPLATADVSQAGVEQFGINLV
                     ANASPVAVGADPDNGSFGFGQAATNYNTPNNYRYVNGDTIALAPKSSGDTVYTISYLV
                     NVSGLTPGGQYTSNQTLVCIGTY"
     gene            2352..>3354
                     /locus_tag="VN778_00150"
     CDS             2352..>3354
                     /locus_tag="VN778_00150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49427.1"
                     /translation="MENSTPTSGDSTQPEPETLKPRNSETAAGAETTEALPAKDGQPV
                     HRRVYRPSHRATFIGLGAVAIVLIINAIIIGLVMRSQSSQQNQPTGQVTINQDALDKL
                     GVNQSGVGGSGVQLTIAPNTNFKGNVVVGGDIQAAGQLKLNGTFQASAARFTQLDAGN
                     ASANQLAVSGTSTLANVVIPTNLNVTGATRLQGAVTVTQLFTVNNSVNISGNLSVGSA
                     LSAGSVSGATVAAISNLVIGGHVISSGSVPSIVRGPNGSNTALGVNGSVSISGNDQAG
                     TVAFNVGVNSNGRGLVATVTFHNLYNSTPHVVLTPIGDVGAFYTSRNSSGFSIYLSSS
                     "
BASE COUNT          801 a          836 c          933 g          784 t
ORIGIN      
        1 taaaagcaaa tagatgcttt acaactccac ctggtttcga acctccatcg gttgcgccgg
       61 cctcgagcgt taggctgcga acggtaatct gatcagcatg tattttcccg ttcgagaaga
      121 ccatgaatag aagcgttgtt atgaagagta tcgctaatag actattggct accctgtggc
      181 ccctcgcact catattcgaa tattagcacg agcgtttatg ccgcgtcaaa aatttttaca
      241 agagcttgta cacagaatgc gctagaacgt cggcgtacag atgtaggtta gggtagtggt
      301 ataggtacca gctggctggc taccagggac gttgacgata tacgatacgg tgaatatttg
      361 cgcgtccgtg ccgcctgctc cgccgaaggc actatccgcc acactattag tggatacaaa
      421 cttgaactga tcgaccgtgc cgtaacccgt tgccggctca cctttatacg taccgccgcc
      481 ggcaccggct gcgggtgcaa cgtctgtccc aacggctggg gtagaagtgg cgaccgtgtt
      541 agcacgaaga ttgaggccaa actgactggt ccctcgtacg cctgccgctg ccgctgccat
      601 agctgtgact gtgttagaac ccgaagtaag cgtcggccca ttaacggtaa tactgtatcc
      661 tgaaccagca ttcgtgctag cggccatttg tgaggttgcg gtagcggtgt cggtgggcga
      721 gaacaattgg ttaaaggtta tcgccccagg agttgctgtt gagcaatccg gcacgccgct
      781 ggtggtgttt acggtggccc ctgcgcaaaa gatcaatgat tcaggcatcg tgcccgttaa
      841 tacgatctgc gttgccgtcg aagctgccac cgtgccacta tccgttatag cgccggtagt
      901 atcaatcgaa gcatacgtag caatacgaac atagaatgtc ttgttcgtag ttgtcgggtt
      961 ggtgaccgtt gacagctcat aggtaaccgc ggtgccagct ggtactgagg ccgctgttcg
     1021 cgtaaggtat ggggcgccgt ttgtcgtatt gaccatcgtg aaacccgtag cgccagtttg
     1081 gttagagagt gttgcagaag ttgtaacgag tcccgtgggc atggtgcagg gatcgctcat
     1141 gactccggcg gcggtgcagt acaaaaattt aattgacccc acgttagtag caccgaccga
     1201 cggcaaagta aaagcaaata gatgctttac aactccacct ggtttcgaac ctccatcggt
     1261 tgcgccggcc tcgagcgtta ggctgcgaac ggtaatctga tcagctgaag cacgactcga
     1321 aaagatcgcc tggagagaca agcccataac caatacgata acaagacttg ctaatccgat
     1381 aggtctgagc cactttgtca tgtgtatcct agaatgtttg ttttagtttg tactaccatc
     1441 ataatggtaa cgtttttgat atgtcaagca gattctagca taagcgaaaa gaggaggtca
     1501 agttagtctg ggctaccggc tcccgctaat aggtaccaat gcacaccagg gtttggttgc
     1561 tggtatattg tccgcccgga gtcaggcccg atacattcac aagataactg atggtgtaaa
     1621 ctgtatcgcc agaactcttt ggggcaagag caatggtgtc accattgaca tatcgatagt
     1681 tgttgggggt gttgtagttg gttgctgcct gcccgaagcc aaacgagcca ttgtccggat
     1741 cagcgccaac agctactggt gatgcattcg cgaccaggtt gatgccgaat tgctcgacgc
     1801 cagcttgtga gacatcggct gttgcgagcg gggtgatggt atggcttcca ttcgttggag
     1861 gatttccgta aatctggacg atgtagccat agctcgtgta gtcaataacc tcaaaagtcg
     1921 aggtggcgac cgttgctgcg gcggctgaga agctgccaaa gttgacgcct gaactgtcga
     1981 cgataaatgt tagcgctggc tcgccggtag tatcaaagcc gctattgagt tgaaaatttg
     2041 tcgatgaaga actaccaact gccagatcac cgatcagctc tccaagttga tagctactgg
     2101 aattctcttg atcgagtcca ctacccccga ccacactctg gtcgaaccgg aagtgtgtgg
     2161 actgcagctc attattatca gcgtaggccg gtacggccat gccaagcatg ataagtaata
     2221 gagtggtggc aagtcccagt aaacgccgca tagtgctatt gtcgagtaag cgtgtgcatt
     2281 ttgcaaacta cggtacttgt ctataaggtt aagcgctata ctacaaacaa gaaagtccaa
     2341 taaataggta tatggaaaac agtacgccaa cttccgggga ttctacccaa cccgaaccgg
     2401 agacattgaa accgaggaat agcgagacgg ccgcgggcgc tgaaacaacc gaagcgctac
     2461 ctgctaagga cggccagccg gtccatcgcc gcgtgtaccg accgagtcac cgggccacat
     2521 ttattggtct gggtgctgtt gcgattgttc ttattattaa tgcaatcatt attggcctag
     2581 taatgcggag ccagagcagc caacagaacc agccgaccgg gcaggtgaca atcaatcagg
     2641 acgcgctcga taagctcggc gttaaccagt ctggcgtggg aggatcgggg gtgcagctga
     2701 cgattgctcc gaacactaat tttaagggaa atgtcgtggt gggcggcgat atccaggcgg
     2761 ccggacagct caaactaaat ggtacattcc aggctagcgc ggcgcggttt acgcagctgg
     2821 acgccggtaa cgcttcggcc aaccagctcg cggtgagcgg tacgagcacg ctagccaatg
     2881 tggttatccc cacgaatctt aacgttacgg gcgcgacgcg attgcaaggg gcagttaccg
     2941 tgacgcagct gttcaccgtt aataacagtg tgaatatcag cggcaattta tctgtcgggt
     3001 cggcgctgtc ggccggcagc gtaagcgggg caacagtggc cgcgatcagc aacttggtga
     3061 ttggcggtca cgttatctca tctggttcgg tgccaagtat cgttcggggg ccaaacggtt
     3121 ccaacaccgc tttaggggtc aatggttcag tgtcgatcag cggcaatgat caggcgggca
     3181 cggttgcttt taacgtcggc gtcaactcaa atggccgggg actagtcgcc acggtgacct
     3241 tccacaacct atacaacagc actcctcacg tggttctcac gcccattggc gacgtcgggg
     3301 cgttctatac cagtcgcaac tccagcggtt tcagtattta tctttccagc agca
//
LOCUS       DATNGP010000008         2810 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_2451512, whole genome shotgun sequence.
ACCESSION   DATNGP010000008 DATNGP010000000
VERSION     DATNGP010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2810
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2451512"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..530
                     /locus_tag="VN778_00155"
     CDS             <1..530
                     /locus_tag="VN778_00155"
                     /inference="COORDINATES: protein motif:HMM:NF012925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="family 16 glycosylhydrolase"
                     /protein_id="HXR49428.1"
                     /translation="DSETWPGDGEIDFPEGNLNGTISAFLHHMNGTSGSDQDAYSSSA
                     TYTSWHTAVIEWTSTQVKFILDGTVIGTSTTRIPSTPMHWVLQSETSTDGTVPSATAQ
                     SNIQIDWVAVYTPSSATTTTTGDLNGDGIVNIFDLSIFLSHWQQTGTGLPEDFNNDGV
                     INIFDLSIMLSHYGT"
     gene            547..>2810
                     /locus_tag="VN778_00160"
     CDS             547..>2810
                     /locus_tag="VN778_00160"
                     /inference="COORDINATES: protein motif:HMM:NF012271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HXR49429.1"
                     /translation="MSTESNTTDKPSKKSRKLPSWLASRKPSAVLVVLAVAVIGSYLI
                     IRSLAATGGSFSVVPATNSVAAGSNFTVTVYEDSGSNPTKSVQADLTYDQNALQFVSI
                     DYSTSAFGTQAESTTGNGVVNMARSSFTDLTGSQVIGVITFKALGAGTTAVKFDAIAV
                     GTGNGNAIVSSTDNSNLLATQTDGSYTITAPADTTAPSVPAGLTVANHTVSSIDLNWT
                     ASTDDVAVAGYKVYRDGTQVGTTPTASFSDSNLTAGTTYTYTVAAYDAAGNDSAQSTG
                     VQATTDANSGGGGSGGGTSSSAAKLYLVPASQSVASGSNLTLNVMVDTGGASVNGVQT
                     TITYPSNQFTYVNLVGDSAFSAFPSPVLGTGTVNFGAGATAAITGVQTVATLTLRATN
                     AGSGNISFQSICTGTSSSTCSAVTDGGANNILQTITGGIFTVTGTTSGGSGGSSGSIG
                     STGGTTSKPAPTKTSTSTKSSTSTRSSTPLAGGTSTVPANSSVPSTSTNPSLTLSGLQ
                     ITNITDTSATITWQTSLPASSEVDYGTYPHFGLVAQDANLVTDHSVTLSAPNITKGST
                     YAIQVVSRDKAGDAVTSDSEQFSTPGFEVTLKLADSHGKPLSHASVTLAEQTKTSDNN
                     GQVVFENILAGDQQVLVKAGNKTTKLSIKVGVYDPATKSYKTQQFSLTAARGTNSTPY
                     VIAGLVVAAIVVIFLLLPQLGAGRHWVPTTVKNVDMDLHGMTVGGNSGPVKPQQNQPV
                     TPSSNSNNKKPGPPP"
BASE COUNT          686 a          821 c          700 g          603 t
ORIGIN      
        1 ctgatagtga aacctggcct ggagatggcg agattgactt cccagaaggt aatctgaatg
       61 gaacaataag cgcttttctg caccacatga acggtacttc cggtagcgac caggacgctt
      121 acagttcgtc tgccacgtac acttcctggc acacggcagt tatagaatgg acatcgacac
      181 aagttaagtt cattttggac ggcaccgtta tcggcacctc cacgactcgg atccccagca
      241 cgcctatgca ctgggtactg cagtccgaaa cctcaaccga cggcacagta ccctcggcca
      301 cggcgcaaag caacatccaa atcgactggg tggcagtcta tactccttca agcgctacca
      361 ccacgaccac tggcgatctc aacggggatg gcatcgtcaa tatcttcgat ttgtctatct
      421 tcttaagtca ctggcagcaa accgggactg ggctgcctga agactttaat aatgacggcg
      481 tcattaacat atttgactta tctataatgc tcagtcatta tgggacgtaa aggttatggt
      541 tgaccaatga gcaccgaatc taatacaaca gacaaaccaa gcaaaaaatc gcgcaagttg
      601 ccctcgtggt tagcttcgcg caagccgtca gccgtcttgg ttgtcttagc cgtggcggtg
      661 ataggcagct atcttattat tagaagttta gcggctaccg ggggctcgtt ttcggtagtg
      721 ccggcgacta attcggtagc ggcggggtct aattttacgg taacagtgta tgaagacagc
      781 ggcagcaacc ccacgaaatc cgtccaagct gatttaacat acgaccagaa cgccctgcaa
      841 ttcgtcagca ttgactacag taccagtgct tttggcacac aggccgaatc aacgactggc
      901 aatggggtgg taaacatggc tcgcagctcc ttcacggatc tcaccggcag ccaggttatc
      961 ggtgttatca cctttaaggc gttgggtgcc ggtactaccg cggtgaagtt tgatgcgatt
     1021 gctgtcggca ccggcaatgg caacgccatc gtgagctcta ccgacaattc caacctctta
     1081 gccacgcaaa ccgacggctc atatacgata actgcaccag ccgataccac cgctcccagc
     1141 gttccggccg gcctgacggt cgccaaccac acggtatctt ctatcgactt gaactggact
     1201 gcttcgacag atgatgtcgc ggtcgccggc tacaaggtct atcgtgatgg tacgcaagtt
     1261 ggcaccactc ctacagccag tttttcggat agcaatctga cagcgggcac aacctacacc
     1321 tacacggtgg ctgcctatga cgcagctggt aacgactccg cccaatcaac aggggtacag
     1381 gccacgacgg atgctaacag tggcggcgga ggaagtggtg gcggtacatc atcaagcgct
     1441 gctaagctct accttgtgcc agccagccag agtgtggcca gcggatctaa tttgacgctg
     1501 aacgtgatgg tggatactgg aggcgcctca gttaacggcg tccaaaccac tattacgtat
     1561 ccgtccaatc agtttaccta tgtgaattta gtgggggatt ccgcctttag tgctttccca
     1621 tcaccggttc tcggtactgg tactgttaac ttcggtgccg gagccacagc agctattacc
     1681 ggtgtccaga cagtagccac cctgaccttg cgggctacga atgccggcag cggaaacatc
     1741 tcgttccaaa gcatttgtac cggtactagc agctctacct gctcagcggt cactgacggt
     1801 ggtgccaaca atatcctcca gactatcacc ggcggtatct ttaccgtgac aggaacaacc
     1861 tcggggggta gcggtggtag cagtggctcg ataggctcca ctggtggcac gacgtctaag
     1921 cctgctccca caaagacttc taccagcaca aagagctcaa cctctacccg gtccagcacg
     1981 cccctggccg gcggcaccag cacggtccca gcgaatagca gcgtgccaag cacaagcacc
     2041 aacccttcac tgacgctatc gggcctgcaa atcaccaaca ttaccgacac cagcgccact
     2101 atcacctggc aaaccagttt gccggcaagt tcagaagtcg attatggtac ctatccgcat
     2161 tttggcctag ttgctcagga cgccaatctt gtgacggacc actccgttac gctctctgct
     2221 cccaacatta ccaagggctc tacctatgcc atccaagtcg tcagccgtga taaagccggt
     2281 gatgccgtca ctagcgatag tgaacagttc agcaccccag gcttcgaagt tacgctcaag
     2341 cttgccgaca gtcatggcaa accgctcagt cacgcttcag tcacgttagc tgagcagact
     2401 aagacctcag acaataacgg ccaagttgtc ttcgagaata ttctcgctgg cgaccaacag
     2461 gtgctcgtga aagctggtaa caagaccact aaactaagca ttaaagtcgg agtctatgac
     2521 ccggcaacca agagctacaa aacgcagcag ttctctttga cggcggctcg cggcacgaat
     2581 agtaccccgt atgtaatagc cggcctggtc gtcgccgcca tcgtcgtcat cttcctgctg
     2641 ttgccccagc tcggcgccgg ccgacattgg gtcccgacta cggtcaagaa tgtcgacatg
     2701 gacctgcacg gtatgacggt aggcggcaac tcaggaccag tcaagcctca gcagaatcaa
     2761 ccggttactc ccagctcgaa ttccaataat aagaagccag gaccgcctcc
//
LOCUS       DATNGP010000009         2154 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_1523024, whole genome shotgun sequence.
ACCESSION   DATNGP010000009 DATNGP010000000
VERSION     DATNGP010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2154
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1523024"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            101..1033
                     /locus_tag="VN778_00165"
     CDS             101..1033
                     /locus_tag="VN778_00165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49430.1"
                     /translation="MKSISKYQMSIAHPSDQINTSGVIPSRSFQAVSDLLKRFSLTCF
                     HDPERVEEYVDLFSLRALCKVLEADRMASKNSALEKSVSALEEKPFEPEYDDLCRLHW
                     IALSRKVINTLEFGSGYSTAILADAMRILNKYFGEWAKNSIRSENPFHVYAVEEEQRF
                     LEITQLRLGAKLQSFATVSRSSVDILTHDNRIVSVYSKLPNISPDFIYLDGPSQFATT
                     QEINGFSFNSKARMPMSADILRVEFFLEPGTLILVDGRTANARFLKSYLRRNWAYLHD
                     PIGDIHYFELQEEPLGPFNKLKLEFCLNGSWLIT"
BASE COUNT          560 a          487 c          487 g          620 t
ORIGIN      
        1 aaccctggcc taaccctggg cttcactata aaaactgggc ctgcccacag ggtcagcgac
       61 agggtcagcg atactatcgg tcaaaatagg aatatttcca atgaagtcaa ttagcaaata
      121 tcaaatgtcg atagctcacc cttctgatca gatcaacact tctggtgtca ttccatcacg
      181 gtcctttcag gctgtcagtg acttgttaaa aagattttca ctcacatgtt ttcacgatcc
      241 ggaaagagtt gaagaatatg ttgatttatt ctctctgcga gcgttgtgta aagtattaga
      301 agcagatcga atggcaagca aaaactctgc gctcgaaaaa tccgtctcgg cgcttgagga
      361 aaagccattt gaacccgaat acgatgactt gtgccgtctt cattggatcg ccttaagtcg
      421 caaggtcatt aacactctcg agtttggcag tgggtacagc acagcaatcc tcgcggatgc
      481 catgcgcata cttaacaaat acttcggtga atgggcaaaa aattccatac gtagtgaaaa
      541 cccttttcat gtgtatgcgg ttgaagagga acagcgtttt ttagaaatca cccaattaag
      601 actcggagcg aaacttcaaa gcttcgccac cgtttctcgc agctctgttg atattttgac
      661 tcatgacaat agaatcgtga gcgtgtattc aaagctaccc aacatatctc ccgactttat
      721 ctatctcgat ggcccctccc agtttgctac tacacaagaa ataaacggtt tctcgtttaa
      781 cagtaaagca agaatgccga tgtcagcgga tattttgaga gttgagtttt ttctcgaacc
      841 aggcacgctt attctggtag atggcagaac tgctaatgcc cgttttttga aatcctatct
      901 acgccgaaac tgggcctatc tacatgatcc aatcggagat attcattact ttgagctaca
      961 ggaagaaccg ctagggccct ttaataagtt aaagcttgag ttttgcttaa atggaagctg
     1021 gcttattacc taaaccgaag aagtagctcc gtttatttag acattgacat cgttaagtgg
     1081 agctttgcgg atgtagagtt attctgacct gcctctgccg ttagcaggga cttagtagag
     1141 cccgtggtag agttataccg taggcatggt tttttcgaga ataataagct caagcgacta
     1201 ttagctgatg cactgctgga taatgcagcg ttgaaagatg tagtttcacg aaagtgatag
     1261 gcccacaagc gcgccggatc agactcaaac aatcagttgt gtgagcacat cgtgtcgctg
     1321 gcgggcaaga gatggcgctt tggttatggt cgtattcatg ttctgcttaa gccggaaggc
     1381 tggcaaatcg acgtcacgcg ggtctacagg ctttattgtc tggccggatt ctcggttcgc
     1441 atcgccagcg cattggcttc atgaaaagat tgccattgtt tcttccggac acgccaaact
     1501 atggctggtc aatggatttc ttccatgatg gtttggccga tggttatcct gcaaccgggt
     1561 ttcggtgatc gtaagttcct tgtgattagt actctaaacc ttggtagtcc gcatcgttat
     1621 ggaattcact ggcggccgtt gtagccgcgc attacatgcg gtcccacttg actattcctc
     1681 tcatcacgtc cttctcggaa tggagcagcg tgttcattat tgccaaaccc tccaccgcgt
     1741 tactgagcgg cgttttctta aatagtattt cgaattcaag ctatgacaag ttccattgac
     1801 tccgctgaac taaagaaact ctaccatcag cggtggcaac ccaacctaaa aattccttta
     1861 ttttatggaa tgtagattgg tttaggagcg gtcgcctgga acgtaagaat ttaaccgcag
     1921 cactttgagt ctactatggt ggattcttta gttaagacaa tatttagctt ttttttttgt
     1981 aacttacctt aaatgtctcc tacagcgtat cgccacttgc cgtaaaattt agggtcaatc
     2041 aattgcgtac ccaggctggc ggttgtccaa gagtgctata tgcccattca gatatttcaa
     2101 gatacggcag cgaaccttgg gtcgagctta tccaaatccc gttctgcgct tgga
//
LOCUS       DATNGP010000010         2559 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_1413458, whole genome shotgun sequence.
ACCESSION   DATNGP010000010 DATNGP010000000
VERSION     DATNGP010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2559
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1413458"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            22..249
                     /locus_tag="VN778_00170"
     CDS             22..249
                     /locus_tag="VN778_00170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49431.1"
                     /translation="MGPTSVGKYVRPLFGPRGTFSIHTADTLEEAIAVAKRTVGGDSA
                     NVLDLREYRVAFTVTADDNLVMTTMAYDSPI"
     gene            574..1287
                     /locus_tag="VN778_00175"
     CDS             574..1287
                     /locus_tag="VN778_00175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49432.1"
                     /translation="MTIQITGEEMAAAKVYTLVGSKIDAKVRAIATLEIADGESGTRT
                     LVVANFGGPNLKAITGHAVLFTLDASASGGYPQGFLSHAGLNSFHPMYVAIRPGGVIT
                     AGRLIYRLNPQAALTKVRFTPFLQPGHIYKIAEGQPGAGGLIVHMRDAVTHQWHDYGS
                     DRKRLQERRPFDGEFHGGFAHVRLYEEHIFTVSNGLAGLETYAFDGIRLERCSLASYL
                     VYGDPGNMAQIEVVDLTAG"
     gene            complement(1514..2095)
                     /locus_tag="VN778_00180"
     CDS             complement(1514..2095)
                     /locus_tag="VN778_00180"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TylF/MycF/NovP-related O-methyltransferase"
                     /protein_id="HXR49433.1"
                     /translation="MISDQVNKDQLAVILTELEKTLSKLDGAVVEFGCYIGTTSLFVR
                     RLLDALKDSREFHVYDSFEGLPPKRAEDESRAGDQFKAGELFVSKKQLIQQFQKASLR
                     PPIIHKSWFNMLASNDVPEKIAFAFLDGDFYESIRDSLKLVLPRMQSGGIIIIDDYTR
                     EALPGAARAANELLSAVQQNRLRVAYNLAIIPV"
     gene            complement(2118..>2559)
                     /locus_tag="VN778_00185"
     CDS             complement(2118..>2559)
                     /locus_tag="VN778_00185"
                     /inference="COORDINATES: protein motif:HMM:NF019614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HXR49434.1"
                     /translation="QTFINSGNLLLRSTETDRAVLTQKIETALEVDFRLSVRVLLKTR
                     SELLSLAAAIPDAWVNDTDTKCDVMFLWPEIDGPQILNELPNNPDIEQTRYEPGAVLW
                     HIDRKLISKSHMTRIVGTRLYHQMTVRNVNTVRKLATLAEAYDK"
BASE COUNT          525 a          645 c          685 g          704 t
ORIGIN      
        1 gctagcgttg agctgttcct gttgggaccc accagtgttg gcaagtacgt caggccgctc
       61 ttcgggcctc ggggtacgtt cagcattcac accgctgaca ccttggaaga ggctatcgcg
      121 gtggcgaagc ggactgtggg cggcgattcg gccaatgtcc tggacctcag ggaataccgc
      181 gtcgccttca cggtgaccgc cgacgacaat ctggtcatga cgaccatggc ctacgactcg
      241 ccgatatagt aggtgcagag agcaccatag gcaggggcgt ggtagcgtgg acgtttggat
      301 ggactatatg tccttctttt ttcgtcctct accgcgcccc tgcctcctgc tctatgatcc
      361 gtatcgctag aagatattta tttgtattat tttatatttt aggttataat atatatcact
      421 ttgtgcttct ttttttgcgc aaagtacctt aacactctct gtaagatgtt tgatggtgca
      481 agttgcttgc gcagctttcc ctatctcgtt agttgtttgt accaccaaat atttctactc
      541 attcaatcgc tccaagaaaa gagctgctgt actgtgacta tacagattac tggcgaggag
      601 atggccgctg ccaaggtgta tacgctggtt ggctctaaga tcgacgcaaa agtgcgagca
      661 atcgccactc ttgagatcgc cgacggtgag tctggcacaa ggacactggt cgtggctaac
      721 ttcggcggcc ctaacttgaa ggctatcacg ggtcacgctg tcctattcac gctggacgcg
      781 tctgcctctg gcggctaccc gcaaggcttt ctgagtcacg ctgggttgaa cagctttcac
      841 cccatgtatg ttgctatccg cccgggtggt gtgatcaccg ctggcaggct catctatcgg
      901 ttgaacccgc aggccgcact gaccaaggtc aggtttactc cgttcttgca gccgggccac
      961 atttacaaga ttgccgaagg gcagcctggc gccggtgggc tgatcgtgca catgcgcgac
     1021 gcggtcacgc accagtggca cgactacggc tcagaccgca agcggctgca ggaacggcga
     1081 ccctttgatg gcgagttcca cggtggcttt gctcatgtgc gcctgtacga ggagcacatt
     1141 ttcaccgtca gcaatgggct tgccggtctt gagacctatg cctttgatgg catccggctc
     1201 gagcgttgtt cgctcgcgtc gtatctggtc tacggggacc ccgggaacat ggcacaaatc
     1261 gaggtcgtag accttaccgc cggctgagat gaccgagtag tacggagagt gtgcggacgg
     1321 ggccggtaga cgggaggact tgagttggca ctatgcccgc ttctattcat ccttattcta
     1381 ctgttgtcct cgtccgtgca ttacatgagt ttataatttt attcttttag tgatgttatt
     1441 tcgccgatac ttatcttggt cgtgcggacc ttcgccctgc caaccttacc tggattgttt
     1501 cgaacggggc tttctaaacc ggaataattg ccaggttgta ggcaacgcgg aggcggttct
     1561 gctgcactgc cgacagtagc tcgttggctg ctctggccgc accggggagt gcctcgcgtg
     1621 tatagtcgtc gatgataata atgccgcctg actgcatcct tggcagcact agctttaggg
     1681 agtcgcggat actctcataa aagtcgccgt ctaaaaacgc aaacgcaatt ttttctggta
     1741 cgtcgttgct tgctaacatg ttaaaccaac ttttatgaat aatcggtggg cgcaagcttg
     1801 ctttttgaaa ttgttggatc aactgctttt tgctgacgaa tagctcaccc gccttaaact
     1861 ggtcccctgc tcggctttcg tcttctgccc ttttgggcgg cagaccctcg aacgaatcgt
     1921 aaacgtggaa ctcccggctg tctttgagcg cgtcaagcag gcgccgaaca aacaaactag
     1981 tagtaccaat atagcaacca aattctacaa ctgccccatc aagcttgctt aaagtttttt
     2041 ctaactccgt aagaattacg gccagttgat ctttattgac ttggtcggat atcaaggggt
     2101 atttggcgag taaagtatta cttatcatat gcttctgcca aggttgctag tttgcgaacg
     2161 gtgttgacat tacggacagt catttggtgg tatagccggg tgccgacaat gcgggtcatg
     2221 tggcttttgc tgattagctt tcggtcgata tgccacaaca cggcacctgg ttcgtatctt
     2281 gtttgttcga tgtctgggtt gttcggtagt tcattcaaaa tttgaggacc atcaatttcc
     2341 ggccacaaaa acatgacatc gcattttgta tcagtgtcgt tgacccatgc gtctgggatg
     2401 gcggctgcca ggctgaggag ttcgctgcga gtttttaaaa gcacccgcac ggaaagtcta
     2461 aaatcgacct ccagggctgt ctcgatcttt tgggtgagta cagctcggtc tgtttcggta
     2521 ctgcggagta gcaggttacc cgagtttatg aatgtttgt
//
LOCUS       DATNGP010000011         3862 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_2685990, whole genome shotgun sequence.
ACCESSION   DATNGP010000011 DATNGP010000000
VERSION     DATNGP010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3862
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2685990"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..736
                     /locus_tag="VN778_00190"
     CDS             <1..736
                     /locus_tag="VN778_00190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49435.1"
                     /translation="ARAYELFEGLQKTIQDGRGWYADRGRTLESSVVYDDKRLQEPSS
                     DMKRTTQAARVITRMIAEFSPGPDRTLLYDPHMGDVRSTPFARYAEALGITSDDSAPE
                     KQFRQLFTHYIVEEEPFPALPPNKLHTALMRGVHAYVHARRGNSLATMIDDNLKDVGT
                     QDLSPLIATGAILAEMMNRNPDVPISYKRSLQGLGSESALICFETIRDAINLADKAKP
                     EKGIVAQLGIFVREHIKQSLLGLEPR"
     gene            852..3329
                     /locus_tag="VN778_00195"
     CDS             852..3329
                     /locus_tag="VN778_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HXR49436.1"
                     /translation="MMPNSPDFQEFLNHLTNNALQSLKHADAIARSVGSAYVGTEHLL
                     LGLLAQDGSMGAKILEGAGVTLDRARMALNLAPKTLVINVGAKGLSETAKLTLKMAYD
                     VAQDYGQEYCGTEHILYSILSQKNARATILLRDMNIDVDNVTGELEQFLNRQQYEENE
                     LNSRRQRTGKKGKKTALDFFGTDLTAQARDGKLDPVVGRENQIRRVITILNRRTKNNP
                     VLIGEPGVGKTAIVEGLAQRIVSEEVPDSLLDKRIVMLDLAGMIAGTKYRGEFEERLK
                     KVMAELEKDNKTIVFIDELHLIVGAGAAEGAMDAGNILKPALARGKIQVIGATTTAEY
                     TKHIEKDAALERRFQPIQVPETTLSETLAILKGLRKHYEDFHGVKLSDEVLEDAVNLS
                     KRYINDRFMPDKAIDLIDETSAHLRVDKGKTSPEVRKLQKELKLVNAQIDEAVDAEDY
                     ERAARFKTRASQIHEKLAQEQANAKDSKRLTVSSEDLADVVARITGVPVKKVIRSEAK
                     YLLTLEKTLGKYVVGQNEAVEAVAKAIRRNRSGIGSSKRPIGSFIFLGPTGVGKTELA
                     RVLAREFFGSENALVKIDMSEFGEHHNVSRLVGAPAGYVGYDDGGQLTDKIRRQPYSL
                     VLFDEIEKAHPDVFNMLLQMLEDGYLTDAKGRRIDFTNTIVIMTSNVGAEKLQKEANL
                     GFHANSTGDLKDLDKLHELNKTRVHEELKKMLRPELLNRIDKVIVFRALTQKDIYRII
                     DLQIDELKQRLQRKGLSVQLASSAKHYLLANGYDAHNGVRPLRRLIQDTIEDQLSVDL
                     LDEKYTKGDIIQVASKNSKLTYATSSE"
     gene            3310..>3862
                     /locus_tag="VN778_00200"
     CDS             3310..>3862
                     /locus_tag="VN778_00200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49437.1"
                     /translation="MPQAQSKNRRPQAGLTRVAALLILLVWLVVLGTAAANRNNIYDW
                     WQLRGYRAPSTVAGLATQDTMTSYARKLFYVNHPAIEDKATFGNKCPNNGGEQTIVLG
                     CYHSDEAGIFLLGVNDPRLAGVEEVTAAHEMLHAAYDRLGTADRNNVDALLMDYYNHD
                     LHDQRLLNTIAAYKKSEPHDLVNE"
BASE COUNT         1053 a          943 c         1019 g          847 t
ORIGIN      
        1 agcgagggcc tacgaattat ttgaaggtct tcagaagact attcaggatg gcagaggctg
       61 gtatgcggac agggggcgta cactggaatc ctcagttgtt tatgacgaca agcgccttca
      121 agagccctct agtgatatga agcgtaccac gcaagctgcc agagtaataa cccgaatgat
      181 tgcggaattc tcgcctgggc ctgatcgtac gctcctctat gacccacata tgggggatgt
      241 ccgctcaact ccgtttgcac gctatgcgga agccctgggt atcacttctg atgacagcgc
      301 tcctgaaaaa caattccggc aattgtttac ccactacatt gtggaagagg agccatttcc
      361 tgccttgccg cctaataaac tgcatacggc tctgatgcgt ggagtgcacg cttatgttca
      421 cgctcggcgc ggtaatagct tagccaccat gattgacgac aacttgaagg acgtgggtac
      481 tcaagacctc agcccgctaa tagcgactgg tgccatcttg gcagaaatga tgaatcgtaa
      541 ccccgatgta ccaattagct ataaacgctc cctgcaaggc cttggtagtg agagtgccct
      601 catttgtttt gaaactatac gagatgccat aaatttggca gacaaggcta agccggaaaa
      661 aggcatcgtt gctcagttag gcatattcgt gcgcgagcat ataaaacagt cattattggg
      721 tctggaaccc aggtaaaaat aaaaaattag cactcttgac gttggagtgc taattgctat
      781 actgggagta ggctatttta cagctagaga catgaaaacg ccgctaaatg taaataggag
      841 aaaaccaagc tatgatgcca aattcacccg acttccaaga atttttaaac catttgacca
      901 acaacgcgtt gcaaagttta aagcacgctg acgctattgc ccggagcgtt ggcagtgcct
      961 atgtcggcac ggaacatttg ctcctcggct tgttagcgca agacggcagt atgggcgcaa
     1021 agattctgga aggtgccggg gtgacgctag atcgggcgcg gatggcgctc aacctggcac
     1081 ctaaaactct agttatcaat gtgggggcta aaggtctcag cgaaacagca aaactgacgc
     1141 tgaagatggc ctatgacgtt gctcaagact atggtcagga atactgcggc accgaacaca
     1201 ttctctacag catcctaagc cagaaaaatg cccgggcgac cattctgctc cgcgacatga
     1261 acattgatgt cgataatgtc accggtgaac tggaacagtt cctaaatcga cagcaatacg
     1321 aggaaaacga gcttaacagc cggcgtcaac gaactggcaa aaagggtaag aaaacggcac
     1381 ttgatttctt cgggactgac ctaacggcac aagcgcgcga tggcaagctt gatccggtcg
     1441 tcggccgaga aaatcagatc cgacgagtaa taaccattct gaatcgccgc acaaaaaaca
     1501 accccgtgtt gattggtgag ccgggtgtgg gcaagaccgc tattgtcgaa ggtttggctc
     1561 agcggatcgt atccgaggaa gtcccagaca gcttgcttga caagcggatc gtcatgctcg
     1621 acttggctgg catgatcgct ggaaccaagt atcgagggga atttgaagag cggctaaaaa
     1681 aggtcatggc tgaactggag aaagataata agaccatcgt tttcattgat gagttgcatc
     1741 tgattgtggg cgcgggtgcg gcagaaggtg ccatggacgc cggtaatatc ttgaagccgg
     1801 cgctggcccg cggcaaaatc caagtcatcg gtgctacgac tactgctgaa tataccaagc
     1861 atattgagaa agatgcggcg ctggaacgac gcttccaacc catccaggtt ccggagacaa
     1921 cgctgagcga gacccttgct attttgaagg ggctgcgtaa gcactatgag gacttccacg
     1981 gggttaaact aagcgatgaa gtgctcgaag atgccgtcaa cctctccaag cgttacatta
     2041 atgatcgttt tatgcccgat aaagccattg atctgattga tgaaacctcg gcacacctaa
     2101 gggttgataa gggcaaaact agtccagaag tccgcaaact gcagaaagaa ttgaaactgg
     2161 tcaatgccca aatcgacgaa gcggttgatg ccgaagacta cgagcgcgca gctcgtttta
     2221 agactcgagc ttcgcagatt cacgagaaac ttgctcagga gcaagctaac gccaaggata
     2281 gcaagcgcct gacggttagc agcgaggatc tggccgatgt ggtggcccgt atcaccggtg
     2341 tcccggtcaa gaaggtaatt cgtagcgagg ccaagtacct tttgacgctc gagaagacac
     2401 tcggcaagta tgtggtgggt caaaatgaag ctgtagaagc tgtggccaaa gctatccgcc
     2461 gtaaccgcag cggtatcggc agcagcaaac ggccgatcgg ctccttcatc ttcctcggcc
     2521 cgaccggtgt tggcaagacg gagctggccc gcgtgctagc tcgggaattc tttggcagcg
     2581 aaaacgccct ggttaaaatc gatatgtccg agtttggcga gcaccacaat gtgtcgcgac
     2641 tcgttggtgc accggccggt tatgttggct atgacgacgg cggccagttg actgacaaga
     2701 tccgccgaca gccctacagc ctggtgctat tcgatgagat cgaaaaagcg caccccgatg
     2761 tctttaatat gcttttgcaa atgctagaag acggctatct gaccgacgcc aagggccgcc
     2821 gaatcgactt tacgaatact attgtcatta tgacgagcaa tgttggcgcc gaaaaattgc
     2881 aaaaggaagc aaatctgggc ttccacgcca acagcaccgg agatttaaag gatctggaca
     2941 agctgcacga gcttaacaaa acacgtgtcc atgaggagct aaagaaaatg ctccggccgg
     3001 agctgctgaa tcgcatcgat aaggtcatcg tcttccgcgc attgacccag aaagacattt
     3061 acaggatcat cgacctgcaa atcgatgagc tgaaacagcg tttacagcgc aaaggactga
     3121 gtgtacagct ggcttcaagc gccaagcact atttgcttgc taatggctat gatgctcaca
     3181 atggtgtccg cccgctccga cggctgatac aagatacaat cgaagatcaa ttatcggttg
     3241 atttactcga tgaaaaatat accaaaggtg atattattca ggtagctagc aaaaacagca
     3301 agcttaccta tgccacaagc tcagagtaaa aatagacgac cacaggccgg ccttacccgt
     3361 gtagcagcct tgcttatttt gcttgtctgg ttggttgttc tcggcacggc tgcagcgaat
     3421 cgtaacaata tatacgactg gtggcagctt aggggctatc gggcgccttc gaccgtagcc
     3481 ggactagcca cgcaggacac tatgaccagc tatgctcgga agctcttcta tgtgaatcac
     3541 ccggctattg aggataaggc gacattcggc aataaatgcc cgaataacgg cggtgagcaa
     3601 accattgtgt tgggttgcta tcatagcgat gaggccggta tttttcttct cggtgtcaac
     3661 gacccgcggt tagccggcgt cgaggaggta acagccgctc atgaaatgct gcacgccgcc
     3721 tacgaccgtt taggcactgc cgacagaaat aacgtcgatg ctctgctcat ggactactac
     3781 aaccacgatt tgcatgatca gcgcctgctt aatactattg ctgcctacaa aaagtcggag
     3841 ccgcatgact tggtgaacga aa
//
LOCUS       DATNGP010000012         2167 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_1676678, whole genome shotgun sequence.
ACCESSION   DATNGP010000012 DATNGP010000000
VERSION     DATNGP010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1676678"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(5..442)
                     /locus_tag="VN778_00205"
     CDS             complement(5..442)
                     /locus_tag="VN778_00205"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HXR49438.1"
                     /translation="MRRAVRAIVFRGNDVLVMKRNKFGKVYYTLLGGGVKFGETPEQA
                     LRREMQEESGLQLGAARLVFIENAGLIFGMQYVYLIDYAGGEPKLDASSDEARIDAIG
                     KNRYHPEWLPVRQLADVPFVSKRLQQALLRALTTRFPAHAEGI"
     gene            complement(574..>2167)
                     /locus_tag="VN778_00210"
     CDS             complement(574..>2167)
                     /locus_tag="VN778_00210"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXR49439.1"
                     /translation="VVFFLTDVGQTVISNISGYLGDIMSTKLQKILGERYYEHVLSLS
                     QSYFDTELSGKIINRMNRGTSQISQFMQMLSNNFLQFVFSTVFSLIVVFIFSWQVGVL
                     LLALYPIFILLTMRTSKKWQVYQGKINADVDVASGRFAEAIGQIRVVKSYLQERRELE
                     FWRGLFDKVISLTRPQSVLWHSQDVVRRLVMNVIFLGVTAYIFIATAQGHYTIGTMVL
                     LIQYAQLIRIPIFTISWLVDNTQRAISNTKDYFEIMDIEPEIADSPKARVLKVTRGDI
                     AFKDVSFAYNHGDKKVLNDISFTAKADSKVALVGESGEGKTTLTNLLLRLYDVHSGTI
                     TIDGQDITAVTQASLRANIGIVFQDPALFSGTIRENISYANPKAGEAAVVAAAKAANA
                     HEFIEKFASGYDTEIGERGLKLSGGQKQRIAIARALLKDAPILILDEATSSLDSRSEH
                     MVQEALRRLMKGRTTLIIAHRLSTIQTVDEIVTLVGGRIGEVGSPERLSRTSGIYAQL
                     LQLQQHGGEADKKKLKSFEIAG"
BASE COUNT          499 a          606 c          535 g          527 t
ORIGIN      
        1 tttcttaaat tccttctgcg tgtgcgggaa aacgggtggt gagcgcccga agcagcgctt
       61 gctgcagccg cttcgataca aacggtacat ccgccagctg gcgcaccggt aaccactcgg
      121 gatggtagcg gttcttgcct atcgcatcaa tccgcgcctc gtcggacgat gcatccagct
      181 tgggctcgcc acccgcgtaa tctatcaagt agacatactg cataccgaag attaggccgg
      241 cattctcgat gaagaccaac cgcgcggcgc cgagctgcag gccagattcc tcttgcattt
      301 cacgccgcag agcctgttcg ggtgtttcgc caaatttgac tcccccgccc aagagcgtgt
      361 agtagacttt accgaactta tttcgtttca taacgagcac gtcgttgcct cgaaagacaa
      421 tggccctcac tgctctccgc atacctcaat tatagggtag aactgacaaa ttccaacccc
      481 tcggctccgt tattccgtac tagatcgaaa atcgagagcc ttccacttcg tagcgaccga
      541 ggttctcgcc ggggcgagaa cggccttcgc aatttagcct gcaatttcga agctcttgag
      601 cttcttcttg tcggcttcgc cgccgtgctg ctgcagctgc agcagttggg cgtaaatgcc
      661 cgaggttcgg cttagcctct ctggactacc aacttcgcca attctgccgc caacgagagt
      721 gacaatttca tcgaccgtct ggatggtgct caagcggtga gcgatgatca atgtcgtgcg
      781 gcccttcatc aggcgacgta gcgcctcctg aaccatgtgt tcactgcgac tgtcgagact
      841 ggaggtggct tcgtcgagaa tcaagatagg cgcatctttc agaagcgctc gagcaatggc
      901 gatacgttgc ttttgaccac ccgataattt caggccgcgc tcgccaatct ccgtgtcgta
      961 accactcgca aacttctcga taaattcatg agcattggcg gccttggccg ctgcgacaac
     1021 tgcagcctca ccagctttcg ggttagcata agaaatattt tcacggatcg tgccgctaaa
     1081 aagcgccgga tcctgaaaaa cgataccaat attagcgcgt aacgaagctt gggttacagc
     1141 agttatatcc tgcccatcaa tcgtgatcgt gccgctatgc acatcataga gtcgcagcag
     1201 cagattcgtg agcgtcgtct tgccttcacc gctttcgccg acgagtgcta ctttgctgtc
     1261 ggctttggct gtaaagctta tatcgtttag caccttcttg tctccgtggt tataggcgaa
     1321 agacacatcc ttgaatgcaa tatcgcctct cgtcactttc agtactctgg ccttcgggct
     1381 gtcggctatc tctggctcga tatccatgat ctcaaagtaa tccttcgtgt tgctgattgc
     1441 ccgctgcgta ttatcaacca gccaactgat ggtgaaaatc ggaattctga tcagctgcgc
     1501 atactggatg agcagaacca tcgttccaat ggtgtagtgc ccttgcgcgg tggcgataaa
     1561 aatataggcc gtaacgccca ggaatataac attcataacc aggcggcgga ctacgtcttg
     1621 gctgtgccag agcacacttt gaggtcgcgt taggctaata actttatcaa acaagccgcg
     1681 ccaaaactcc agttcacgcc gctcctgtaa gtagcttttc acgacgcgga tctggccaat
     1741 agcctcggca aaacggccgc tcgccacgtc cacgtcggca ttgatcttgc cttgatagac
     1801 ttgccatttt ttgctggtac gcatagtcag caggataaaa attggataca gcgccagcag
     1861 gagcacgcca acttgccagc taaaaataaa caccacgatc agcgagaaaa ctgtgctgaa
     1921 aacaaactgc aggaagttat tgctgagcat ctgcatgaac tggctaatct gtgaagtgcc
     1981 gcggttcata cggttgataa tcttgccact cagctcggtg tcaaaatacg actgcgacaa
     2041 gctcagcaca tgctcgtagt agcgttcgcc caggattttc tggagcttgg tcgacatgat
     2101 gtcacctagg tagccgctga tgttgctaat caccgtctgg ccgacgtcgg tcaggaagaa
     2161 cacgacc
//
LOCUS       DATNGP010000013         3322 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_2766394, whole genome shotgun sequence.
ACCESSION   DATNGP010000013 DATNGP010000000
VERSION     DATNGP010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3322
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2766394"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..699)
                     /locus_tag="VN778_00215"
     CDS             complement(<1..699)
                     /locus_tag="VN778_00215"
                     /inference="COORDINATES: protein motif:HMM:NF013106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HXR49440.1"
                     /translation="MSAAPNRGRNRRSRNTVTTKSGNTIKVNTSLSDRIKSRRLARAT
                     DKATYLSTLPKDRWKRFLYRLNPKQQAQFWFSREGALMALKIIGVAIVVGFFMTIGLF
                     AYFRKDLPKIKDISGDNLGGSITYYDSTGQTVLWQDYSAVKRIPVQSDQMSPYMKKAT
                     VAIEDKDFYKHGAFDVRGIMRAAYHDAVGGGGSVQGGSTITQQLVKLNENWTNNRTVT
                     RKVKELILAVELERE"
     gene            851..1273
                     /locus_tag="VN778_00220"
     CDS             851..1273
                     /locus_tag="VN778_00220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49441.1"
                     /translation="MRILKPLVAAWSFVVVGALVIAVSSLLATHYLSAQDTANKAKFV
                     TCKQTGAAHAVILQNDKAKPQHTFGKLCDTMTISNEDSQPRLIAFGPHEEHQPYDGVT
                     ERILGQGQSLTVTFNEVGTYQFHDHIADIIQGDFTVVK"
     gene            1278..1877
                     /locus_tag="VN778_00225"
     CDS             1278..1877
                     /locus_tag="VN778_00225"
                     /inference="COORDINATES: protein motif:HMM:NF019499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HXR49442.1"
                     /translation="MANKKTLKEVLPYILLIGGVVGIFCAGILTYDKIESLQHPGAHL
                     NCNLNPIVACGPVINKAQASAFGFPNPFIGLVGFTVVATTGGAILAGATFKRWYWLCL
                     EAGVLFAAAFVTWLQFETIYRIGALCPFCMVTWAATLPIFWYTTLYNFREGHITTPSK
                     FKPTLAFAQKHHGDILILWFLLILTAITTHFWYYWRTLF"
     gene            1955..3205
                     /gene="tyrS"
                     /locus_tag="VN778_00230"
     CDS             1955..3205
                     /gene="tyrS"
                     /locus_tag="VN778_00230"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HXR49443.1"
                     /translation="MTLSEELTWRGFVNQTTYKDIAVLDQAPITFYWGVDPSADSMTI
                     GNLAAAMMVRHFIDHGHKAVLLVGGATGLIGDPDGKSQERELLSTEDIERNKQKIGEQ
                     YQTIFAGQAFEIVDNYDWFKNLGYLQFLRDVGKHVPMRQMLGREFVQTRLGEDGAGIS
                     YAEFSYSLIQGYDFLHLHREHGVTLQVCGADQWGNSIAGVELIRRIDGQEAHVWSAPL
                     VVNKTTGVKFGKTEGGAIWLDSQKTTPTQFYQFWINCDDEGVEDYLKVYTLLNKEEIE
                     QIMAEHRQDPGRRVAQKRLAAEVTKLVHGDKQMPIAEAVTAYLTGHKSIGEASPEEMQ
                     SLRQEIPSVLAHAGDCILDVLVQSGLTSSKGEARRVQASGGVYINGQTAPGEQLESNL
                     FDNGRLLLRRGKRFEDSALVELGE"
BASE COUNT          840 a          780 c          835 g          867 t
ORIGIN      
        1 ttcgcgctcg agttcgacag ccaaaatcaa ctcttttact ttacgggtga ctgtacgatt
       61 attggtccag ttttcattta acttaacaag ctgctgagtg atcgtcgaac caccctgcac
      121 cgaaccgccg ccgccgacag cgtcatgata ggcggcacgc ataatacccc tcacatcaaa
      181 cgccccgtgt ttatagaagt ctttatcttc gatagccacg gtggcttttt tcatataagg
      241 ggacatctga tcggactgca ccggaatacg tttgacggca ctataatcct gccacaacac
      301 cgtctgcccg gtactgtcat agtacgtaat gctgccaccg agattatcgc cggatatatc
      361 cttaatcttc ggtaaatcct tacggaaata ggcgaagaga ccgattgtca tgaaaaagcc
      421 gacgactatg gcgacgccaa taattttaag cgccatgagg gcaccctcac gactaaacca
      481 gaactgggct tgctgcttgg ggtttaggcg gtatagaaac cgcttccagc gatctttcgg
      541 cagggtactg agatacgtcg ccttgtcggt ggcccgtgcc agacggcgcg atttaatgcg
      601 atcgctgagc gaagtgttga ccttgatggt atttccgctt ttggttgtta cagtatttcg
      661 ggaacggcga ttacgccccc ggttcggtgc agcgctcata gactaagttt cggtctcccc
      721 atgccacatg atttatgccc attatagcaa acccggccgc aaaaaagcct acgttttaat
      781 tcataatttc tcagctttcg ctcagcctag ccgcaaaatt cccaagactt ttaagctata
      841 gtattactat atgagaatct tgaagccgtt ggtggcagcc tggagtttcg ttgtcgtggg
      901 cgcgctagtg atagccgttt cctcgctgct cgccactcat tacctgtctg ctcaggacac
      961 cgctaataag gcgaagttcg tcacctgtaa acaaacgggg gccgcgcacg ctgtcatcct
     1021 gcaaaacgat aaggccaagc ctcagcacac atttggtaaa ttatgtgaca ccatgaccat
     1081 ttcgaatgaa gatagtcagc cgcgtttgat agccttcggt ccgcatgaag agcatcaacc
     1141 gtatgatggt gtaaccgaaa gaattctggg ccaaggacaa agcctgacgg taacttttaa
     1201 cgaagttgga acttaccaat ttcatgatca catcgccgac ataatccagg gtgatttcac
     1261 ggtagttaaa tagctaaatg gcaaataaga aaacactcaa agaagtttta ccgtatattt
     1321 tattgatcgg tggtgttgtc gggatttttt gtgccggtat attgacatat gacaaaattg
     1381 aatcgttaca gcaccccgga gcccatttaa actgtaatct caaccccatc gttgcgtgcg
     1441 gtccggttat caacaaagca caggcatccg cctttggttt tcctaatcca tttatcggac
     1501 tggttggctt taccgtggtt gcgaccaccg gcggcgcgat cttggcaggc gctaccttca
     1561 agcgctggta ctggctttgc ctggaagcag gtgtactctt tgccgctgcg tttgtgactt
     1621 ggctccagtt tgagactatt tatcgtatcg gggctttatg ccccttctgt atggtgacgt
     1681 gggcggccac gctgccgata ttttggtaca ccactttata taactttcga gaaggtcata
     1741 taacgacacc gtcaaaattt aagcctacgc tggccttcgc ccagaagcac catggcgata
     1801 tcttgatcct gtggtttttg ctgatcctta cggcgatcac tactcatttt tggtactact
     1861 ggcgcacttt gttttaaagc agagccgcta ccgccttgcg atttgaagga gcttttgagt
     1921 tatactaggg aaaacagatg tacaaggata ttacatgacc ctttccgaag agctgacctg
     1981 gcgcggtttt gttaaccaaa cgacttataa agatattgca gtgcttgatc aagcaccaat
     2041 tacattttat tggggcgtag atccgagcgc tgattctatg actatcggta atttagcagc
     2101 agctatgatg gtgaggcact ttattgatca tgggcacaaa gcggtgctgc tggtgggggg
     2161 cgccactggg ctgattggtg atcctgatgg caagtcgcag gagcgtgaac tgctatcaac
     2221 tgaggatatc gagcgtaata agcaaaaaat cggtgagcaa tatcagacaa tcttcgctgg
     2281 gcaagcgttt gaaattgtcg acaattacga ctggttcaaa aacttgggtt atttgcagtt
     2341 tttgcgtgat gtcggcaaac atgtgccaat gcgtcagatg ctagggcggg aatttgtgca
     2401 aaccaggctt ggcgaggatg gtgcgggcat tagttatgcg gaattcagct attctttaat
     2461 tcagggctac gatttcttac atctacaccg cgagcatggt gttacgttgc aggtctgcgg
     2521 cgccgatcaa tggggtaaca gcattgctgg ggtagagctt attcgccgca tcgatggtca
     2581 ggaagcgcat gtctggtccg ctccgttggt agttaataag actactggcg tgaaatttgg
     2641 caaaactgaa ggtggcgcca tctggcttga ctctcagaaa accaccccga cacagtttta
     2701 tcaattctgg atcaactgtg acgacgaagg cgttgaggat tatctgaaag tttacaccct
     2761 actgaacaaa gaagagattg aacagatcat ggctgaacat cgccaggatc cgggtcggcg
     2821 cgtggcacaa aagcggctgg ctgccgaggt tactaagctt gtccatggtg ataagcagat
     2881 gcctatcgca gaggcggtaa cggcttattt gacaggccac aaatccattg gtgaggcaag
     2941 cccggaagaa atgcaaagtt tgcgccaaga aataccgagt gtgttagctc atgccggaga
     3001 ctgcattctg gatgtattgg tacaaagcgg tcttacaagt tctaagggtg aagcgcgtcg
     3061 agtccaggcc tccggcggcg tatatattaa tggtcaaacc gcccccggcg aacaattaga
     3121 gagtaatttg tttgataatg gtcgtttact tctccgaaga ggtaaacgtt tcgaagactc
     3181 ggcgctcgtg gagcttggag aataactgag ccctgttata ctgatgtata aggaaagcct
     3241 atgcccaaac agcaaacctc accgaaaact gctcatgcca aacagaagcg gcgtgccgat
     3301 caatacaatg atccgagcca ta
//
LOCUS       DATNGP010000014         8155 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_3223161, whole genome shotgun sequence.
ACCESSION   DATNGP010000014 DATNGP010000000
VERSION     DATNGP010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 8155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8155
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3223161"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(21..1298)
                     /locus_tag="VN778_00235"
     CDS             complement(21..1298)
                     /locus_tag="VN778_00235"
                     /inference="COORDINATES: protein motif:HMM:NF013377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase family glycosidase"
                     /protein_id="HXR49444.1"
                     /translation="MAFDKAKDLVTKTIKLSFGAERDLTPEDVRLARDYIAAYWSKIE
                     RFHPKDDESLLGLPKPYLVPSYREKTGFDYNELYYWDSYFMVQGMLDEAHKELVMGIL
                     EDLVSLFERFKVIPNASRTYLTGRSQPPFLTTFIFDTYQAYDPGEAWLKKMMGVAKLE
                     YEIVWMGMKKPNERQVYQGLSRYYDINYLHDLAEAESGWDMTPRFDRHALHYLPADLN
                     ALLYKYEADFARAARIFGDVREAAHWDDASRVRKAAMDKLMWDRIRGLYYDYNFKKEK
                     RGGVSSLATYFPMWAGMVDEKQAASLVKALRRFENKGGVATTDALPLGQFVIGTMPTQ
                     WAYPNGWAPLHFVVIKALERYGYHKDARRIAMKWLKTNLAWFNDNHVFLEKYNVVAPD
                     KPPLKGVYPSQTGFGWTNGVFERLCQDYVDRPE"
     gene            complement(1363..1668)
                     /locus_tag="VN778_00240"
     CDS             complement(1363..1668)
                     /locus_tag="VN778_00240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49445.1"
                     /translation="MKLRNNQAGFSAFELILVVLVLSALAAVTLRVLDNHNKAVNSST
                     AATSPAVTKSVSDVSSAPVVNKASDLNKAEDTLNQNDPTAANQADSNQLDGQLSGLN"
     gene            complement(1668..2315)
                     /locus_tag="VN778_00245"
     CDS             complement(1668..2315)
                     /locus_tag="VN778_00245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49446.1"
                     /translation="MNRMKRLSKLTLLLTALFTLSLASVPVYAKDGPVSGSGDASEGG
                     TTAASATTETETGDTTSGTPETHHGKSQNSVETANTASTDAKQKQCDRRQKTVETILS
                     HIATRGQKQIDLFGTIATRVENFATTNNKQPSNYDSLVAAVNTAHDNAVAAVADIQTK
                     STSVNLNCNSPTGSKGVAESFKAELKSEISALQAYRTAVKNLIVGVKSSGTGGSN"
     gene            complement(2449..2733)
                     /locus_tag="VN778_00250"
     CDS             complement(2449..2733)
                     /locus_tag="VN778_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HXR49447.1"
                     /translation="MATKLFVGSLAWATTDDSLKDLFSSVGTVVSANVIFDRETNRSK
                     GFGFVEMSSDEEAKAAVDQLNGKDLDGRPIVVSEARPREERPRPSFDKRY"
     gene            complement(2997..3293)
                     /locus_tag="VN778_00255"
     CDS             complement(2997..3293)
                     /locus_tag="VN778_00255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49448.1"
                     /translation="MPLPPDEPNDEERLEELPGDEGETPFNPGGPIRDDETSDPEEAR
                     SEKLGDTDPPTDSGLEAEDIYENGLPEAAGAEEPNAGNAVVDYDPTKDHRDKPK"
     gene            complement(3574..4245)
                     /locus_tag="VN778_00260"
     CDS             complement(3574..4245)
                     /locus_tag="VN778_00260"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HXR49449.1"
                     /translation="MKNKYVWVVVVGIVIIGLGGGCFWWWPGHHKAQPLNDGAASINT
                     DSTVAQTNSTNGGLSVAPTSGAQNLGQLSGSQKSSNNVTAQGTSSNSSSGSSGAVDPS
                     TFSQYDKYKSGTSALFGDIQVGTGAELGANQQAAVYYKGWLTNGQLFDESRTGSDGKL
                     QPFTFTLGAHQVIPGWEQGLSGMKVGGTRLVIVPPAVGYGSQAQGSIPANSVLVFEVQ
                     LVAVQ"
     gene            4707..4880
                     /locus_tag="VN778_00265"
     CDS             4707..4880
                     /locus_tag="VN778_00265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49450.1"
                     /translation="MKLHKKSPNNIGHFLVEHLEKSSPLIIEEIHRWSDLLWEDWMDH
                     QVEQKRRKKLPIP"
     gene            complement(4914..6698)
                     /locus_tag="VN778_00270"
     CDS             complement(4914..6698)
                     /locus_tag="VN778_00270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49451.1"
                     /translation="MSKRKQVTLLIIIVAIDVFLVFLKFNLKLSHYTLPVASNPYFTL
                     GDAVAALALVFAVTQLANDSWGLTLRIKGRVKSNIVWLLLGLGLAASLTSALLPQPSS
                     TAIKVYKNPLFWQIAAFFCFASAPFVLRRIGTYRRRLFNKSTAPRYYQVLLAAYSTGD
                     NKQIKAATDIILESLESIVECIKPTHFKVTLRMRFLSEINKNKFGQRYILWLNKRRFA
                     KEERKRKEKKGTSPQQYAYRLFDTALTDTVVSSYIATKRIDFIWNFTRSITSKRLEGR
                     QLRRGFNSIVSQLFTNENSYLYRQSKYKGTGQFAPVNDLIFGSKYLAQNFEPIRSWRE
                     GRSSTDIKPEDYVEVFLRALESSIKNDAFNDSRIAESVGFIMYELSEYTRDLLYKSED
                     SINEYHMAFWRIEQFFSRTFLELYSEALKANKVSDNEKAATKGERYRQQCLTATYAEC
                     FVDFLGELSRGPKDGMSMHQLVVFALNELYEKNEYSAIRDTFALYAWEAIDMSVKQGF
                     YPAFIKIYIRLMYFMSSKDGAWVKAERAKLMKLVNGDLKAKIKARAKMNNKRDYMEDY
                     LLPSELKYNRKKDKFEWTDAGDNKSDFN"
     gene            complement(7761..>8155)
                     /locus_tag="VN778_00275"
     CDS             complement(7761..>8155)
                     /locus_tag="VN778_00275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49452.1"
                     /translation="VIVILSVIGGLISNSSQSNNQPQTGASTSEVSKFTQLNSCLDNV
                     DSWWNANATTNGMALDLLPNYQQQVNQCKTEYPVAASPAYQSQNEACINNVDQWWGKE
                     ATTVGLADMMLPVKTEMVTECSTRYPNS"
BASE COUNT         2001 a         1951 c         1887 g         2316 t
ORIGIN      
        1 cgcaacccat tttcgaaggg ctactccggc cggtccacat agtcctggca caaccgctca
       61 aacacaccat tggtccagcc gaagccagtt tgcgacggat atacaccctt taaaggtggc
      121 ttgtctgggg caacgacatt gtatttttcc aggaagacat ggttgtcgtt gaaccaggca
      181 aggttggttt tgagccactt catagcgatg cgccgggcat ctttgtggta gccatagcgc
      241 tccagggcct tgatgaccac aaagtgcaac ggcgcccagc cgttcgggta ggcccattgg
      301 gtcggcatgg tgccgatgac gaactggccg agcggcaaag catcggtggt ggccacaccg
      361 cctttattct caaagcggcg caaggctttg accagcgaag cagcctgctt ctcgtcgacc
      421 atcccagccc acattgggaa gtacgtagcc aaactgctga cgccgcctcg cttttctttt
      481 ttgaaattat agtcgtagta tagcccgcgg atacggtccc acatcagttt atccattgca
      541 gctttacgaa ctcggctagc gtcgtcccaa tgtgccgctt cgcgcacatc gccaaaaatc
      601 ctggcggccc gcgcaaagtc agcttcgtat ttgtacaata acgcattgag gtcggctggc
      661 agataatgca gggcgtgccg gtcaaaacgc ggtgtcatgt cccacccgct ttcggcctct
      721 gccagatcgt gcagataatt gatgtcgtaa tagcggctca agccctgata aacttgccgt
      781 tcattcggct tcttcatacc catccagaca atctcatatt cgagcttggc cacgcccatc
      841 atctttttga gccaggcctc gcccggatcg taagcttggt aggtgtcaaa aataaaggtc
      901 gtcaggaaag gtggctgtga gcggccggtc aaataggtcc gggaggcatt gggaatgact
      961 ttgaaacgct caaagagaga cactaaatct tctagtatgc ccatgactag ttccttatga
     1021 gcttcgtcca gcatgccttg caccataaaa tagctgtccc agtagtacag ctcattgtaa
     1081 tcaaaaccgg ttttttcgcg gtacgacggc accaggtacg gcttcggcaa gccgagcaga
     1141 ctttcgtcgt ctttcggatg aaatcgctcg attttggacc agtacgcagc gatataatcc
     1201 cgagccagac gaacgtcttc gggcgtcagg tcacgctctg ccccaaacga gagcttgatt
     1261 gttttggtaa ctaaatcttt tgctttatcg aacgccatac gccttctagc ctaccggcta
     1321 cacacgtagc cggtcaacct aaaatccatc tttagagtgc gcttaattca gaccagacaa
     1381 ttgcccgtcc agttgattgc tatcagcctg gttggcagct gtcggatcat tttggttaag
     1441 cgtatcttcg gctttattga ggtcactggc cttattgaca acaggcgcgg aggagacgtc
     1501 actgacactc ttggtaaccg ccggtgatgt tgcggcggtg ctgctgttga cggctttgtt
     1561 gtgattatcc agtacgcgga gggtgactgc agctagtgcg ctgaggacaa ggacaaccaa
     1621 gattaattcg aaggccgaga agccggcctg attgttgcgc agtttcatta gtttgatcct
     1681 cctgtcccac ttgatttgac gccgacgatc agattcttaa cagcggtgcg ataagcctga
     1741 agggcggata tttcgctctt gagctcagct ttgaatgact ccgcgacacc cttcgaacca
     1801 gtcgggctgt tacagttcag attaacactt gtgctctttg tctggatgtc agcgacggcc
     1861 gccacggcgt tgtcatgggc ggtattaacg gcggctacca agctatcgta attagagggc
     1921 tgcttattat tggttgtagc gaaattctcc acccgggtgg cgatagtgcc gaacaagtca
     1981 atttgttttt gcccgcgggt agcgatgtgg ctaaggatgg tttcgactgt cttctgccgg
     2041 cgatcacact gcttttgctt cgcatccgtg ctggctgtat tcgcagtttc gactgaattt
     2101 tggcttttac catggtgagt ctctggagtt ccactggtgg tatcaccggt ttccgtctct
     2161 gtcgttgcag atgcggctgt agtaccacct tccgaggcat cgcctgatcc cgaaactggt
     2221 ccgtccttgg catagacggg gacgctggcg agactcagcg tgaagagcgc cgtcaggagc
     2281 aaggttaatt ttgacagtct tttcattcta ttcatgtcct ttttgtgttt gttttatttc
     2341 tgctaactgc tttaagctta tgctcagggt aatatgttgt caagaagttg ggtcatacag
     2401 aaaagagcag gtacggatac ctgctctttt gattcagcca gcgagcgttt agtaacgctt
     2461 gtcaaatgaa gggcgaggac gctcttcgcg cggccgagct tcgctgacga cgatcgggcg
     2521 accgtcgagg tctttgccgt tcaactggtc gacagcagct tttgcttctt cgtcagatga
     2581 catttcgaca aaaccgaagc ctttgctgcg attggtttcg cggtcaaaga tgacattagc
     2641 cgatacgacg gtaccgacgc tcgaaaacaa atccttcaag ctgtcatcgg tagttgccca
     2701 agcgagtgag ccgacaaaca atttggttgc cattgatatt actctcctaa cggtccgagg
     2761 taatctggca aaccagttag ctgctcctca ggaaacacgc acttattact taattaactc
     2821 tagaacttaa cggtaacgtt attagcagct tttctagcct cgctagtata acagatctag
     2881 atcaagagca caatgccgat ttagcctaca ttgacttgcg gcgagcggct tgcgcttttt
     2941 tgatattgcg cttggcggct tcacgctgtt tttgagtggc cataagctcc tccttatcac
     3001 ttaggtttat cacgatggtc tttggtcgga tcgtaatcca caaccgcatt gccggcattt
     3061 ggctcttccg cgccggcggc ttcaggcaaa ccgttttcat aaatatcttc cgcctctagc
     3121 ccactatctg tcggcggatc ggtatcccca agcttttcgg atcttgcttc ctcgggatcg
     3181 cttgtctcat catcacgaat gggaccacca gggttaaaag gtgtctcgcc ctcgtctccc
     3241 ggcaattctt cgagccgctc ttcatcattc ggttcatctg gtggtagtgg catgttgctt
     3301 agtatgccta agcggagaag tgaaaacagt taaatagcgt acagacgact cgcaaaagcc
     3361 ctttacgttt aggcaaaccg tagtaaaata tgagtattag caattgaaac aaagaaaggg
     3421 ggtaatccat tgaaaagtcc tagatctaac gggcaggcaa tgaatgttat agcgcgggag
     3481 gtacgcgtct agcagccctt tactagacta ttcgcctgcc taagtaggcc gaataccttg
     3541 agccgctttc atctcggaag cggcttttcg tttttattga acggcgacga gttgcacttc
     3601 aaaaaccagg acagaattcg ccggtatcga accctgtgcc tgcgaaccat aaccgactgc
     3661 cggcggcacg atgaccaatc gcgtaccacc gactttcatg ccgcttaagc cttgttccca
     3721 tcctggaata acctgatgag cgcccagcgt gaacgtaaaa ggctgcagtt tgccatcgct
     3781 accagtccgc gactcatcga acagctggcc gttcgtcaac caacctttgt aatacaccgc
     3841 cgcttgctga ttagccccca gctctgcgcc agtgccgact tggatatcgc caaacaaggc
     3901 actggtgccg gacttatact tgtcatactg gctgaaagtc gaggggtcca cagcaccgga
     3961 agaaccggaa ctactgttcg aacttgtacc ttgcgcagta acattattcg aagatttctg
     4021 cgagccactc agctgcccca agttctgcgc tccgctcgtc ggcgccacgc ttaaaccgcc
     4081 attagtactg ttagtttgcg ccaccgtact atcagtgtta atcgaagcag ctccatcatt
     4141 aagcggttgc gccttatgat gcccaggcca ccaccaaaaa cacccaccac caagcccaat
     4201 aatcactatg ccaacgacga ccacccacac atatttattt ttcatgccta attaaattgt
     4261 aagccatatg cttcgtgatc ccaagcaaga tcaaaatcgg gtaaaaaagt acaagctctt
     4321 gaaagtcgtt ctatctagta aatgagtaag aagctagttg actgatcttc tttcgtctaa
     4381 ctcattagct cggctaattg cgttttgagc taaacgctca aaagcgctta cgtaccattc
     4441 actctctcct ctgctattag cctgaagagc cgtgcgcgtg gctagccctg acactactct
     4501 taacattgag gtattgtccc agttgcttgc agcctcgtaa agaatttcta catcgctcgg
     4561 agatgctcta cgtaatagag ctcgttgctc tggagtatgt tcatcagggg atgtcatacc
     4621 ggtaaaccac acatcacttt tatggcgatc ttcttcgtat tgccttacgg tatacagcat
     4681 tatcgagaaa ttatactcgc tttgtagtga agctgcataa aaaaagcccg aataatatcg
     4741 ggcacttttt ggtggagcac ctcgaaaaaa gttcacccct catcattgaa gaaatacata
     4801 gatggagcga tttactctgg gaagattgga tggaccatca agtagagcag aagcggcgaa
     4861 agaagttacc tatcccctag cggtatacac ccttgtgcct ttaagctagt aatctagtta
     4921 aaatcagatt tattatcacc cgcgtccgtc cactcaaact tatctttctt cctgttgtac
     4981 ttgagttcgc ttggcaacaa ataatcttcc atgtaatcac gcttgttgtt catctttgcc
     5041 ctagctttta tctttgcttt taggtcacca ttcactagct tcatgagctt agcacgctct
     5101 gccttgaccc atgcaccgtc ctttgagctc ataaagtaca tcaagcgaat gtatatcttt
     5161 ataaaggctg ggtagaagcc ttgcttaacg ctcatatcaa tcgcctccca agcatagagc
     5221 gcaaatgtat ctctgattgc actatattca ttcttttcgt aaagctcatt aagtgcgaaa
     5281 acaacaagct ggtgcatact catgccatcc ttggggcctc ttgatagctc acccagaaag
     5341 tctacaaagc attcggcata ggtagctgtt aggcactgct ggcggtaacg ttcacctttg
     5401 gtggccgcct tttcattatc ggatactttg ttggctttta aggcttcact ataaagctct
     5461 agaaacgtac gactaaagaa ttgctctatg cgccaaaatg ccatgtggta ctcgttaatg
     5521 gagtcttccg atttgtacag caggtcacgc gtgtactccg aaagctcgta cataataaaa
     5581 cctactgatt ctgcaattcg gctgtcgtta aaggcgtcat tttttatcga actttcgagc
     5641 gcgcgaagga aaacttcaac gtaatcctct ggctttatat cggtactcga ccttccttca
     5701 cgccatgagc gtataggctc aaagttttga gccaagtatt tggagccgaa tattaaatca
     5761 tttacaggcg cgaactgacc agtacctttg tatttggact gcctatacaa ataggagttt
     5821 tcgttggtga acagctgtga aacaatacta ttaaagccac gcctaagctg gcgaccctct
     5881 aagcgtttac ttgtaatact cctagtgaag ttccatataa aatctatgcg tttggtagca
     5941 atatagctac tgactacagt atcggttagc gccgtgtcga acagtctgta ggcgtattgc
     6001 tgtggcgatg tacctttctt ttctttacgc ttacgttctt ctttagcgaa acgacgctta
     6061 ttcagccata gaatatatct ttgaccgaat ttatttttgt ttatctcaga taagaatctc
     6121 attcgtaaag ttaccttaaa gtgtgttggc ttaatacact ccactattga ttcaaggctt
     6181 tcaagtatta tgtcggtagc tgctttaatc tgcttgttat ccccggttga ataggcagca
     6241 agcagcacct gataataacg cggtgctgtt gatttattga acaagcgtct tctataagtt
     6301 cctatacgtc gaagcacgaa gggtgcgctt gcaaagcaaa agaatgcagc aatctgccaa
     6361 aatagggggt tcttataaac tttaatcgct gtactgctgg gttggggtaa gagtgcggag
     6421 gttagggaag ctgctaaccc caatccaagc agtaaccaaa ctatgttact tttcactctt
     6481 cccttaatcc tcaaagttaa tccccacgaa tcatttgcaa gctgtgtaac cgcaaaaaca
     6541 agggcgagag ctgcaacggc gtcacctaaa gtaaagtatg gattactagc caccggtaaa
     6601 gtgtagtgtg acagttttag attgaacttc aagaacacca agaacacatc tatcgcaacg
     6661 atgataatta gcagtgtcac ctgtttacgc ttgctcatag tgagtattta tacagtaaac
     6721 tgcaatcgag cgatattggt tatggctaat cttgcagtcg aggagatgag gttttatgtc
     6781 gcacaatgaa cgttgtcgga cagtagctaa caggggtagc ttgtgatttt atgtatttcc
     6841 aagctttgtc aaattataaa gttactaatg ctacccctgt taaaaaatat attatacaat
     6901 aatcactttt gctatatatt aattcgggtt ttcaagctcc tggatgcgtt gttcgaggtc
     6961 ttctacttta gcatcgacaa ctttggcaat tcgcactatt gatttactta tattatttat
     7021 acgcacatat aaatcaacaa taatgaaaat tactattaga attatccaaa tcattctttc
     7081 ccctttctct ggttatcaat taagagcaca tattccctgt cagttgcgga atgtaagagt
     7141 tatttatagt atttatctgg ctcactatag agttgagttg ctgttggtcg tgctgtagct
     7201 gtgcctgttg tgccgatgta ttcgtggggg tcgattggcc ataaccgttc tgccaactat
     7261 acgaactatt atttgtgatg tagctctgtt cagtttggat ttgtgacctt agttgtgccg
     7321 cttgagcgtc gaggctagcc gttgcattag ccatttgagt cctaatgcca gcgcatttac
     7381 tagggtcagg tgtagcgata ttagatgtac taggagcatt actgccgctg ttaggtatgc
     7441 tagatgggct ataggtatag ctactgctgt agtcaccgtt ggttggtgca ggctgtgttg
     7501 tggctccaat atttgttgta ccttgaccta aggcagagga aggcgctgga gcgctgcctg
     7561 tagcgttgtt aataggcgta gtagagtccg ttaaattaac agaagcattt gagctgtgag
     7621 ggtgggaagc taagctactt agaccaataa tggcagtggc aaatataaga attgatatcg
     7681 aaacatttct aattttcttc ttacgatatc ctgcatgtag ctcgtcgagg tcaaggaatt
     7741 gttccattta ttttctgctc ctagctattt ggatagcgtg tactgcattc tgttaccatc
     7801 tcagttttga cggggagcat catatcggct aggccgaccg ttgttgcttc tttaccccac
     7861 cattggtcaa cattatttat gcaagcttca ttctgcgact gataagctgg cgaggcagct
     7921 accgggtact ctgttttaca ctgattaacc tgttgttggt agttaggcaa taggtcaaga
     7981 gccatcccgt tagttgtcgc gtttgcattc caccacgaat caacattatc aaggcaggag
     8041 tttagctgtg taaatttgga gacctcagag gtgcttgcgc cagtttgggg ctgattattg
     8101 ctttgagagg agtttgatat aagaccacca ataaccgata aaatgacaat aacta
//
LOCUS       DATNGP010000015        21899 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_745552, whole genome shotgun sequence.
ACCESSION   DATNGP010000015 DATNGP010000000
VERSION     DATNGP010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 21899)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21899)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21899
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_745552"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1024)
                     /locus_tag="VN778_00280"
     CDS             complement(<1..1024)
                     /locus_tag="VN778_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HXR49453.1"
                     /translation="MNQDHIRNFCIIAHIDHGKSTLADRLLEVTGTVDKRDMKAQLLD
                     RMDLEREKGITIKLAPVRMQYKDYELNLIDTPGHVDFSYEVSRSLEACEGALLVVDAS
                     QGIQAQTLANVYLAMAAELTIIPVLNKIDLPAADVERVSKEIMSLLGCLQEDILLISA
                     KTGQGVEVVLDSIVDLVPAPTPSNALEARALIFDSYYDDYRGVILYVRIFDGAIHKNS
                     QIKMMATNATGLALEVGSLKPDMVPGSSLQTGEIGYIVTNLKSTREAKVGDTVTLTNQ
                     SATQQLPGYKDVQPFVYAGFFPESNEFYQELKDAIEKLSLSDSALQFAPENSPVLGFG
                     VRIGFLG"
     gene            1164..2039
                     /locus_tag="VN778_00285"
     CDS             1164..2039
                     /locus_tag="VN778_00285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49454.1"
                     /translation="MSEGTNGWNRVFKPLDGEPAAAGALSSTSGYALVTSGEAAHSAA
                     PKTPDSPYEVTVVGQVDGGLKAKHEKAGFPVPRVLSSKSERTVLFAPQVSVNYVTYLE
                     GIREADVHLPIWVYLTTRRTQTQPKAAISKNNNAGIITLPITSNFATGNQEEVRGVNE
                     MLWRATLAADRGSDPKVANRASRIHNANTRIKDLLWFGGIGGLAVVGATEAGEALSAA
                     TAIPPHAIHFPEVVQRITEFSVPTFVAGLIGRMAIEDESRPLQLGINKSLKSRAIARQ
                     AARMAADFKVLESPL"
     gene            2281..2688
                     /locus_tag="VN778_00290"
     CDS             2281..2688
                     /locus_tag="VN778_00290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49455.1"
                     /translation="MDPEDTKASSTTESSIIFTPAPTSLEVNTGCTVIKLTPQESVPT
                     IEVHGIVVTYDSSAGMLTITGDAKVTYEVDDGVLKLTLLPTRTVLNNAMAKLALDGMS
                     SILGSRRSGLGGLFGDPLPDYSDYGPGRKSSMW"
     gene            complement(2877..3629)
                     /gene="radC"
                     /locus_tag="VN778_00295"
     CDS             complement(2877..3629)
                     /gene="radC"
                     /locus_tag="VN778_00295"
                     /inference="COORDINATES: protein motif:HMM:TIGR00608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HXR49456.1"
                     /translation="MQGTVMRNYALEDRDLIFSEPGAGYVLRVKDMPDDERPRERLLE
                     LGPSSLSVAELVAILWGVGNRKEDVLAMAKRTLKEYGEKAIGNELNAQRLAETTDIPV
                     TKATQVVAAFELGRRFYATQAGRPVQVRNAKQAYQYVRDMGISQKEQLRGLYLNSRYQ
                     VIHDEVISIGSLTSNIVHPREVFRPALERGAVAIILAHNHPSGRLEPTLADIQITEQL
                     LTAGDLLGIELLDHLIITTTQHASILDCISRD"
     gene            complement(3694..5289)
                     /locus_tag="VN778_00300"
     CDS             complement(3694..5289)
                     /locus_tag="VN778_00300"
                     /inference="COORDINATES: protein motif:HMM:NF015013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HXR49457.1"
                     /translation="MNPTTPAPKAKRPTISNVAALLIATSFAGQVLGFLRTKLVNANF
                     PTTGPHSTDAYFAAFAVPDFFFFTLAAGALGVAFMPVLSDRLHRGDRRGMWEISNSLM
                     NMLAIIMGVVAIVMLIFAKPLIRHIVAPGLSPGQLNTAADIMRLLAFNPLLFTISGIL
                     TSVQQTMGRFFFYAIAPLFYNVSIILSIYLFKHNIGLVGLGLGALLGAILQLLVVACG
                     LWKTNFHWRPHIGWRSADFRLVLKNLPPRSLDQGVDQVENIVETHIASGLGSGNVTYY
                     NNAYILSTAPILLIGTAISTAAFPRLNARLSQHRPDLFRRDFLMVLRAMIWLSAPLVV
                     VCFFGRGYLARLIYTKGNFQIATIFGFLTVAIFFRIVYSIISRWFYAQKDTKTPLFVS
                     IFTIGLNVVLAVTLARPSQYGVSGLALAQSIVAAVEVFILSVIMLLRDHRLFDAVFWG
                     GVWRIISVTGFSVVAGFIMISLYPLGRNDRGILTLGSKLALIAFVTFAVHIGISALFD
                     LEEVRPLFTRLKKLALKPVKLEV"
     gene            complement(5370..7235)
                     /gene="ftsH"
                     /locus_tag="VN778_00305"
     CDS             complement(5370..7235)
                     /gene="ftsH"
                     /locus_tag="VN778_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010165504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HXR49458.1"
                     /translation="MDRKQLRSGRKPGSNNRGMKNAGFIALIILFGLIVFAAANQPSN
                     LQKIPITTAVTEANAGKYQKITVTGNQLDITKKGETHATLKSYLEPNATLKDEGFNFS
                     KVEIDTTPASSGTSTWVSVAETVLPTLAIVGLLYFMLRSAQGQGNQALSFGKSRARLY
                     GNEKDKATFKDIAGSDEAKQDLEEVVEFLKFPKKFAGMGARIPKGVLLVGPPGTGKTM
                     LARAVAGEANVPFFSISGSEFVEMFVGVGASRVRDLFAKAKKNSPCIIFVDEIDAVGR
                     RRGSGMGGGHDEREQTLNQILVEMDGFEQGQNVIVLAATNRADVLDPALLRPGRFDRR
                     VNIGLPDRKDREAILKVHFAKKPLAKNVDLDALAAKAAGSSGADLANIANEAAIIAAR
                     NSHTEISQADVTEAFERVAIGPERKSKVMTEADKVTTAYHEAGHALVGHVLPDSDPVH
                     KVTIIPRGGTGGVTWFIPPEDRVYVSLIQFKDMLARGLGGRIAEEVVLGHDRITTGAG
                     SDLQKAAELARDMIVNQGMGKKLRDQVFHVDDGMMLDRLVHEREYSDETAKVIDEEVE
                     NLITEAANRARAVVKANLDKLEVLKDRLLDKETVDADEVTEALRGAKMPQAASLY"
     gene            complement(7270..8175)
                     /gene="tilS"
                     /locus_tag="VN778_00310"
     CDS             complement(7270..8175)
                     /gene="tilS"
                     /locus_tag="VN778_00310"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HXR49459.1"
                     /translation="MDIVLPKPGTYIVAVSGGVDSMALLHMLYSEAVSNSRPWKLIVA
                     HLDHGIREDSAEDRRLVQAAAGRYGLPFVYHKSHLGAGTSEAEARKARYEFLHKVRGA
                     SGARAIITAHHQDDVLETAIINLLRSSGRKGLTSLKSHHDIERPLLQVPKRELIAYAK
                     DQGLVWHEDSTNQNLDYLRNYIRHRLLPKFDKTARDKLHAIIAAQHQTNNELDTLLVN
                     QLHLQSVGGRLDRIWFNHLPHAVAREVMAEWLRAHGVRNFDRKTLERLVVAAKVSPKG
                     SRYNVLQDRFMQVNSSDLALVRAER"
     gene            8195..8482
                     /locus_tag="VN778_00315"
     CDS             8195..8482
                     /locus_tag="VN778_00315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49460.1"
                     /translation="MAERSDLLKRIGKLEARNKRVEGDKAWETSWVRRSSIILLTYLT
                     VAFYLHFVVHISPWINALVPVIGYTLSTLTVQQLKQRWLDQQKSKRTSKQG"
     gene            8521..9294
                     /locus_tag="VN778_00320"
     CDS             8521..9294
                     /locus_tag="VN778_00320"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HXR49461.1"
                     /translation="MYHDLILFLTGIIVGAMNAIAGGGMLIGFPVMLALGIPALVANA
                     TTFVIVIPGNISSIYGYRQLLHKVPRHYAWLLIPTVMGAFIGAQALKHTSQGGFAHLI
                     PWLILFAVVLFAFQPWLYKQLHQHLHGPKRKRNSLRPLWITGLALLPIATYGGFFATG
                     FGFIMLAFLGFTGLHNHIHRMNALKNIIGLCLATTALLCLANAHVIDWRHGLVMGAGN
                     FIGGYFAATRTQKVSSHALRIIVIIVGACAAIYLCLRNY"
     gene            9341..9417
                     /locus_tag="VN778_00325"
     tRNA            9341..9417
                     /locus_tag="VN778_00325"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9375..9377,aa:Met,seq:cat)
     gene            complement(9442..11715)
                     /locus_tag="VN778_00330"
     CDS             complement(9442..11715)
                     /locus_tag="VN778_00330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49462.1"
                     /translation="MIRLETQQLSGFNDTLIDEPAHFEVTLPNGQVRDYDAVIGNTPA
                     VTSEYDAYDQPQLVLQTEVPDVLPWDEEVHYPAGRLASFHGRTLEILAGKVAVEDQFG
                     NHYQSVSIKGSDFSDPHIFSSETASRGFIIHGLQESLVLERVIRASRLLREHGIPAEY
                     ICGLVIPDKFPMHTDPDNPDTRQLVPLPELVEALAADYAQEELEESRTPLEVKADMIE
                     KLHDVDYLITYRAMDAPYRFGELADPDTLDEFYDFLIRQNDDPNLHEYLEELHPMTYI
                     VAQFAVELGINVAKMHKLGVSHGFLHSENITALGSFVDLDSCRGEPLGLGDDPITDKD
                     IYKDIVWAMRSLVDVLDGWQPSPHDDINGFLDITSMQVYAREFFLTEYLKERSFDKSA
                     AEAFLTGLVSYQQPKRTQHYGSFARFCEVVTGAYCSFIGGEEHIKDVNEEDMPSIENY
                     DLHYDRKDTVFRAAPARFFKEFRDRLLSNNWSRNHEYESLHETDKPLHSLMKAAIRSD
                     SLRFFVRHAEQSGLIDEPALFMAALTMGSSITNVDPENVPNKNQALHQYFVRQGEKMV
                     EQLGDEYTQYDKPVMEFLLNKSLGDLKKQTGILDWENGAITSLLYLNGATQYERIFKA
                     LDLQPQSKITYINVGEAMRRAVNKQDDTILILDYGVAEFIAETPRKDDLWKAVFGYRH
                     KFKPLILIEGLLAGEPEICLVHYEELEDARYKNLEGEEGDNEEPEKLKPELPETVAGD
                     LSLDTLRQNRLRLFSTD"
     gene            complement(11721..12113)
                     /locus_tag="VN778_00335"
     CDS             complement(11721..12113)
                     /locus_tag="VN778_00335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49463.1"
                     /translation="MGEFEANSRQVISQEDFMRVADGLLAHAEDPSKDVNECLVSAQT
                     LVQGLDDVKAGNLHVEPPLSEEQVSSYRKQSQLVIALGQKSVRALQATSERRGFLRRR
                     QEPPEPPTPTAQQPARPAYSGTGSDAKK"
     gene            complement(12280..13059)
                     /locus_tag="VN778_00340"
     CDS             complement(12280..13059)
                     /locus_tag="VN778_00340"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HXR49464.1"
                     /translation="MKWPNTLTVVRHGQSAYNELKGLKEGDPLYAEFKRAHNRRKKDP
                     DTARRLAEQLIQDGTFILGCGDHDTRLTQTGERQAEVTGSRLSELIGLPDVIFVSPYL
                     RTHQTLGKLAVGWPELSSVKTVEEERVREQEHGLALFYNDWRIFNIMHPEQEALRNLQ
                     GSYWYRYPQGENVPDVRERHRSMLGALTRDYAEQNVMIVGHHLSILSLRANLERLDAD
                     GFRDLDANHKPVNCGVTVYRGNPDVGHDGHLELDIYNQQLY"
     gene            complement(13067..14656)
                     /locus_tag="VN778_00345"
     CDS             complement(13067..14656)
                     /locus_tag="VN778_00345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49465.1"
                     /translation="MTKQESAFRQEQSPAQEMRDTLYHRNSFLNRPDEAYYFVGNQAT
                     EILHGYYKNDESVTEQSQTGLKYWVALENQFATAVVGHRMPKARIGRQASYYAVMQAR
                     LDEKGSEADIAGVVEAAQALSPEETWEYFLDQLAAYEAYLKGDLTKPVNRETGQPNTQ
                     DASFYMDLAEALWISIKYVMQNAPTKEHHSRTNRLNQQLVNISNTFLDANKVGLAIGF
                     GSIISGAEGQDKAFAANLVVKMQELRSVESDNNFRHVYNYATQTLGWSQLRLFKQQLP
                     KEATPLIFGSESEVLYADSDSPMLTERFEEGELLGASLSDGSRILLPHINYRHRTILG
                     YSTEQESEIYEVQTGVNYETLSDEAKAAVEIALNAEEQGQLGNEARPRYRRLAFWHDN
                     DKGDLLYTVIFRRERGRLMDLYPDHHGLATTDTERKLDMPVRSAEAVKDTYMVVPYDE
                     SYMAFQTFTDAQPNLFATGVNQANAFWLMGGPRTIHSQADSWLMKEAEDGQQPKYVIV
                     PIRYYKIEEDFVTPDPSGANE"
     gene            complement(14762..15034)
                     /locus_tag="VN778_00350"
     CDS             complement(14762..15034)
                     /locus_tag="VN778_00350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49466.1"
                     /translation="MLLIIHLTCAFASLAFMAGAVIGSWRHRGGNYDNLARLSGLSFG
                     GLLTTRTALAIKYRTGLMSVCLSVLFYLACLTALFTLYRKLVQSAG"
     gene            15151..15990
                     /locus_tag="VN778_00355"
     CDS             15151..15990
                     /locus_tag="VN778_00355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49467.1"
                     /translation="MAIQQAGQEHLTTLTAQWELTVLGHSSTQDASFVINHTKPEVLV
                     ATGFGETDISQVAERLGHLGIRAAVFNLAYPQTVGGEDLKLTPAIMEQTVLKSVSAIA
                     QEINMFMGYDAQTPMSMVGHSKGFGELLKFVSAYPEACDNLAGAAPLGANCIPKIMGS
                     TPKEYRANFKTRLRESGLLSSLVSHPEAGRLGNEFMAGIQYLHQEDAPALIRNLHGMG
                     ILKSIVIGSDDALCPPAECEQAIGIAGLIVTTAGKHDHISTPEGMAQIALAIEQCINL
                     ETR"
     gene            15987..16667
                     /locus_tag="VN778_00360"
     CDS             15987..16667
                     /locus_tag="VN778_00360"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HXR49468.1"
                     /translation="MKLKHVKGIIWDLDGTLIDSFEIFEQIIADVVETSGHSMPTHEY
                     MLSNYHGSLEETIQKILGLESAAEVDEIISIFAEKQEHHYAGDLETHLFQDAAMLAQQ
                     AAKLGMQQLIVTNRDHEGRGTGSPRAIIAATTLADCIHEIRSGDEVDFRKPDKRSVGD
                     WMERHQLTPAEVVVIGDQFVDAQLALNIGARAVLIKRNGDIPHLDTLEDHEDVVIVDS
                     LEIVELVR"
     gene            16746..17144
                     /locus_tag="VN778_00365"
     CDS             16746..17144
                     /locus_tag="VN778_00365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49469.1"
                     /translation="MKSSNISQIKKYYLLWNPQITNLSGHGKLKLNSTYSTTSNVLGV
                     TIKSKNVITKFRADKLIQIENKTGLVHYIARFTLKPDRKKTLVICATTVSSESSAFAF
                     AVPVLKLLARRELQTDLQALKLACEHQLLQ"
     gene            complement(17167..18234)
                     /locus_tag="VN778_00370"
     CDS             complement(17167..18234)
                     /locus_tag="VN778_00370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49470.1"
                     /translation="MSEQNIRQQQAAVEQETFRKEDKAETQKIVESPIESVQKLPTKP
                     SIVNRLTPRPFITPTQKSGPKEQPRKPAQIASTEHKPDFVTQVVPKPSLKSVSPEIVR
                     AHEAVNGVPESQLEAAGSEASFAFENDEARSVSLEAEAIDFATELGLASDETEDDSWI
                     DTLIIPGIMSEAENQEAVTPEALSESRTIVLTKIAEVIAPPESVAEKTEVLLSHLPEV
                     VQISLAEFVQESEPEVAEAVSELVVQMALATNRLQVLIMTDRAESEEAAQIEIVLEEW
                     YDTLLTYLEIEDAEAKVAEFIRLVRSGNYVVLQQPMPENTDEGTHERKFNVPLTQGLS
                     RIMQQVQTAIGKLAVGQAVYL"
     gene            complement(18405..18782)
                     /locus_tag="VN778_00375"
     CDS             complement(18405..18782)
                     /locus_tag="VN778_00375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49471.1"
                     /translation="MLYEGLSSPAQEPQSIADLDPQTRAELEGVFAKTSLEQFQNETG
                     FGVAELAQYVTYETTEGVQTATVATMLSHDSCPMGKWTRNAYKKGGLAEVQDMYQDLS
                     KREPGLNIVVSEQVLARENPKKK"
     gene            18972..20126
                     /locus_tag="VN778_00380"
     CDS             18972..20126
                     /locus_tag="VN778_00380"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014151870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S53 family peptidase"
                     /protein_id="HXR49472.1"
                     /translation="MGRKVYILLVLILGLAFSAGGGRASAAVHSRKVCSISHAAGTAR
                     CHAIILTDGSNVTPLATTAPQGYGPATFKAAYGNSGRSTTHVAVVVAYDAPTIASDLA
                     IFSRTFGLPVLPTCTSAAQSSCFEKLNQRGSSSYPAVNSSWAVEASMDVESVHGQCPG
                     CRISLVEANSASMANLSAAVDEAVATGAKVTSNSYGGAESSGEAAYDRHYHQAGVTMV
                     VSSGDSGYGTDYPAASPYVIAVGGTSLKMNGSKVASEIAWDGAGSGCSLFENKPSWQH
                     DKKCVMRSIADVAADANPSTGAAIYDSYGSGGHHGWLTVGGTSLAAPLVTGIIAASGN
                     SSNVSILLYGHTTNVIRDIVGGSNGTCVSYLCKALAGYDGPTGLGVLNHL"
     gene            20179..20808
                     /locus_tag="VN778_00385"
     CDS             20179..20808
                     /locus_tag="VN778_00385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49473.1"
                     /translation="MRFNKQLFKNGWLLFACGLALGVLVILAIRFFTYKPVQVHYHAN
                     FAVYLNGQRQEFKGPQYYQEVAICSSTNDITIPQQRAHMHDNINSVVHVHDHAVTWGQ
                     FFENLGWYVGPNFIETDSGTLYTENDQDKLHILINGQDYTDLTPITNMLIKDKSRLLI
                     SFGDISDSTLQQEYKTVPSTAARFDATQDPSSCSGTEKVTVKDRFHHLF"
     gene            20812..21408
                     /locus_tag="VN778_00390"
     CDS             20812..21408
                     /locus_tag="VN778_00390"
                     /inference="COORDINATES: protein motif:HMM:NF013346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HXR49474.1"
                     /translation="MHIGIILLVFAVIFIAELPDKSLFASLILGSKFPALYVWLGCAA
                     AFLVHVIIAVLAGQLLTLLPHLVLHIIVGILFLAGALLIFFGKHGLEEATHHEPKPRK
                     VVAPNFWKVFGTSFTIIFLGEWGDITQISVANYTAHYHDSLNVGIGATLGLWAACGLA
                     VTAGSKVISRVPAKILQRTTGVILILFAIISFATAVQK"
     gene            21478..>21899
                     /locus_tag="VN778_00395"
     CDS             21478..>21899
                     /locus_tag="VN778_00395"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HXR49475.1"
                     /translation="MKTALGILVVVLILIGGYAVVKHNDKNAPNTSTSSNSGGNSSNM
                     TMSSNQSSSSASQTNAVNITNFAFSPSNITVKKGTAVTWTNKDSVAHTVTENDGQDGP
                     KSSNLDQNQTYSFTYNTVGTFKYHCSIHPDMTGSVTVT"
BASE COUNT         5380 a         5723 c         5314 g         5482 t
ORIGIN      
        1 agcccaggaa accgatccgc accccaaacc ctagcaccgg cgaattctct ggtgcgaatt
       61 gcaaagccga atcactgagt gagagcttct caatcgcatc tttcagttcc tgataaaatt
      121 cattactttc cggaaaaaaa ccggcataca cgaaaggctg cacatctttg tagccaggta
      181 actgctgagt agctgactga ttggttagcg taaccgtgtc gccgactttg gcttcccgcg
      241 tactcttcag atttgtcacg atataaccga tctctccagt ttggagcgac gaaccgggca
      301 ccatatcggg ctttaatgaa cccacctcca aggccaggcc ggtagcattc gtggccatca
      361 tcttgatctg agagttcttg tgaatggcac cgtcaaaaat tcgcacgtat aaaatcacgc
      421 ctcggtaatc atcgtagtag ctgtcgaata tcagagcccg ggcttccagc gcattcgacg
      481 gcgtaggcgc cgggactaga tccacgatac tatcgagcac cacctcgact ccctgccctg
      541 tcttcgcgga aataagcaag atgtcttcct gcagacagcc aagcaagctc ataatttcct
      601 tgctgacccg ctcgacgtca gccgccggca agtcaatctt attgagcacc ggaataatcg
      661 tcaactctgc cgccatcgcc aaataaacat tggctagcgt ctgcgcctga atcccctggc
      721 tggcgtcaac cactagcagt gcaccttcgc aagcctccaa actccgcgaa acttcatagc
      781 taaagtccac atgccccggc gtatcgataa ggttcagctc ataatctttg tactgcatgc
      841 ggaccggcgc cagcttgatg gtaatcccct tctcccgctc caaatccatc ctatcgagca
      901 gctgggcttt catgtcccgc ttatcaaccg tccctgtcac ttccagcaac cggtcagcca
      961 acgtcgactt cccgtggtca atatgagcaa taatgcaaaa attgcgtatg tggtcttgat
     1021 tcatttagag atatattata acagaggtgg gcggtagaat actaatctgt ttgtctttgg
     1081 ggaaagcaag gccaccctca gtgtttacac tatgtattga caatatccat attctatatt
     1141 actatatacg aatgtctgaa ggtatgtctg aaggtacaaa cggctggaat agagttttta
     1201 agcctcttga cggagagcct gcggctgccg gtgcgctctc aagcacctcc ggctatgcgt
     1261 tagttacttc tggcgaagca gctcattcag cagcccctaa aacccctgat tcaccttacg
     1321 aagttacggt tgtaggtcaa gtagatggag gattgaaggc aaagcacgag aaggccggct
     1381 ttccggtacc cagagttctg agcagcaaaa gcgagcgtac agtgcttttt gccccacaag
     1441 taagcgttaa ttatgtaact tatctagagg gcattaggga ggctgacgtt catttaccaa
     1501 tctgggtgta cttaaccacc cgaagaacgc agacacagcc taaggcggct attagcaaga
     1561 acaataatgc gggcataatt acactgccca taacttcaaa ttttgcaaca ggtaaccaag
     1621 aggaggtgcg gggtgtcaac gaaatgctct ggcgtgcaac gctagccgct gaccgaggtt
     1681 ctgatcctaa agttgcaaat cgtgcgtcta ggatacacaa cgccaatact agaattaaag
     1741 atttgctttg gtttggaggt attggaggct tagctgtagt gggtgcaacc gaagcaggcg
     1801 aggccttaag tgctgcaacg gccatccccc cacacgctat tcatttccct gaggtagtgc
     1861 aacgtataac tgaattcagt gtacccacct ttgtagcagg tttgattgga agaatggcca
     1921 ttgaggatga gtctcgtcct ctacaattag ggataaacaa gagcttaaag agtcgtgcaa
     1981 tcgcgcgaca ggctgcacga atggcagcgg attttaaagt tttagaatcc cctctttgac
     2041 agttcatcaa aactaggcat gcaaccttcg ttagctattg acttattata tattttatgc
     2101 taatatatat gcactttgtt gaacggaggt gtactctctg attccaaagt accttgacaa
     2161 gtaggcaggc taagaggtgt agagcgagtc catacgacac gtgagcttac tctacatctc
     2221 taacaatcgg taggattcca cccatccgtc agggctttgc cctagaaggg aaggtagtgt
     2281 atggacccgg aggacacgaa ggcctcatcg acgaccgagt cgtcgatcat attcaccccc
     2341 gcgccgacgt cgctcgaggt caacaccggc tgcaccgtca tcaagctcac cccccaggag
     2401 agcgtcccga ccatcgaggt ccacggcatc gtggtcacct acgactcctc ggccggcatg
     2461 ctgacgatca cgggcgacgc caaggtcacc tacgaggtcg acgacggcgt gctgaagctc
     2521 acgctgctgc cgacgcggac agtcctcaac aacgccatgg ccaagctggc actcgacggg
     2581 atgtccagca tcctcggctc gcgccgttca ggtctcggcg gcctcttcgg cgacccgctt
     2641 ccggactact ccgactacgg gcccggtcgc aagagcagca tgtggtagcc gtcctcggcg
     2701 acgcgcaatt ctgagcacca ggcgggtgtg gatacgcttc aagctgttca cgcccgccgc
     2761 gtgcccctcc catacccttt tcctacttat tccgcctgct ctacttgtct tttccgcttt
     2821 cataagtgcg tgcttatgct gacgagtcca aaaggacgaa aagcggaatc gttagattag
     2881 tcgcgactga tgcagtccaa aatactggcg tgttgagtgg tagtgatgat caggtggtcg
     2941 aggagctcga tgccgagaag gtcgccggcg gttaggagct gctcggtgat ttggatgtcg
     3001 gccaaagtag gttctaggcg accggaaggg tggttgtggg cgagaatgat ggctacggca
     3061 ccgcgctcga gagcaggacg aaagacttcg cgcggatgga cgatgttaga ggtcagactg
     3121 ccaatagaga tgacctcgtc atggatgacc tgatagcggc tgttgaggta gaggccgcgg
     3181 agctgctctt tctgactaat acccatgtcg cgtacatatt gataagcctg cttggcgtta
     3241 cgcacttgaa ccggtcggcc ggcttgcgta gcgtaaaaac gacggccgag ctcaaaggca
     3301 gcaacaactt gagtggcttt tgtaaccggg atgtcagtgg tttcggccag acgctgggcg
     3361 tttagctcgt tgccaatggc cttttcgcca tattctttga gggtgcgctt ggccatggcc
     3421 aagacatctt ctttgcgatt gccgactccc cacagtatcg ccacgagttc ggccaccgag
     3481 agactgctcg ggccaagctc cagcaatcgc tcgcgggggc gttcatcatc aggcatatct
     3541 ttgacgcgca gaacatagcc ggcccccggc tcagaaaaga tcaggtcgcg gtcttctaaa
     3601 gcataattgc gcattactgt cccttgcatg gtcatcatta taccttcaag gtactagaag
     3661 aatattaaat aattattaaa tattgccaaa ttactacact tctagtttga ctggtttcag
     3721 tgccagcttc ttcaagcgcg taaacagggg cctgacttcc tccaagtcaa agagggccga
     3781 gatgccaata tgcaccgcaa aggtgacaaa agcgatgagc gccagcttgg agccaagcgt
     3841 caagataccc cggtcgttgc ggccgagcgg atacagcgaa atcataataa agccggccac
     3901 cacgctaaac ccggtcaccg atataatacg ccagacaccg ccccaaaaca ccgcgtcaaa
     3961 caagcgatgg tcacgcagca gcatgatgac agacaagata aacacttcaa ctgcagccac
     4021 tatagactgc gctaaggcga gccccgagac gccatactgg ctgggacgag ctaaagtaac
     4081 ggctagcacc acgttgaggc caatggtgaa aatcgagaca aacaacggcg tcttggtatc
     4141 tttctgcgca taaaaccaac gtgaaataat ggagtacacg atacggaaaa agatagcgac
     4201 tgtcaggaag ccaaagatgg tggcaatctg aaagttgcct ttggtgtaga tcagccgcgc
     4261 caaatagccg cggccgaaaa agcagaccac gacaagtggc gcactcaacc atatcattgc
     4321 tcgtagcacc atcaggaaat cacgtcgaaa tagatccgga cgatgttggc tcaagcgagc
     4381 attgagccgc ggaaaggcag ccgttgagat cgccgtaccg atcagcagga ttggcgccgt
     4441 gcttaaaata taggcgttgt tgtaataggt gacattcccc gagccgagcc cactggcgat
     4501 atgcgtttcg acgatgtttt cgacttggtc aacaccctgg tccagcgagc gtggcggtaa
     4561 gtttttgagc accaaacgaa aatccgcgct gcgccacccg atgtgcggac gccagtgaaa
     4621 attcgtcttc cataaaccgc aagccaccac tagcagttgg agaatagcgc caagcagcgc
     4681 tcccaagcct agcccgacca acccgatatt atgcttgaaa agatagatac tgaggattat
     4741 cgatacgtta taaaacagcg gcgcgatggc ataaaaaaag aagcggccca tggtctgctg
     4801 cacgctcgtt aaaataccgg aaatagtgaa caggagcgga ttaaaggcca gtaagcgcat
     4861 aatgtcagct gcggtattga gctggcccgg cgacaagccc ggtgcgacaa tgtgcctgat
     4921 gagcggtttg gcaaaaatca gcatgacaat cgccaccacg cccataatga tcgccaacat
     4981 gttcataaga gaattggaga tttcccacat accgcgccgg tcaccacgat gcaagcggtc
     5041 ggacagcacc ggcatgaaag ccacgccaag cgccccagcc gctagtgtaa agaagaaaaa
     5101 gtccggcacg gcaaaagccg caaagtaggc atccgtgcta tgcgggccgg tcgtcggaaa
     5161 gttggcgttc actagcttgg tccgcaaaaa ccccagaact tgacccgcga acgatgtcgc
     5221 gatcaatagc gcggccacat tgctgatagt gggccgcttt gccttgggcg ctggcgtagt
     5281 gggattcatg cagcgttatt aaccatcata aacttctatc cagtataaag cccgcatggt
     5341 gacagatcaa aaccgattta aaactgaact tagtagaggg aggctgcctg tggcatcttg
     5401 gcgccgcgta aggcttcggt gacttcgtcg gcgtcgacag tttctttgtc gagcaagcgg
     5461 tctttaagaa cttctagctt gtcgaggtta gccttgacga ctgctcgggc gcgattggcc
     5521 gcttcggtga tcagattttc gacttcctca tcaataactt tggctgtttc atcggaatat
     5581 tctcgttcat gcaccagacg atcgagcatc ataccgtcat cgacgtggaa aacttggtcg
     5641 cgcagctttt tacccatgcc ctgattaaca atcatgtcac gcgccagttc agcagccttt
     5701 tgcaggtcac tgccggcgcc ggttgtaatg cgatcgtggc ctaacactac ttcctcggcg
     5761 atacgaccgc caagaccacg ggccaacata tctttgaatt ggataagcga tacgtagacg
     5821 cgatcttccg gcggaataaa ccaggtcacg ccgcccgtgc cgccacgggg gataatcgtt
     5881 actttatgga caggatcgct atctggtaag acatgcccga ccagcgcatg gccggcttcg
     5941 tgataagccg tcgtgacttt gtcggcttcg gtcatgactt tgctcttgcg ttctggcccg
     6001 atagcaaccc gttcaaaggc ttcggtaacg tcggcttgag aaatctcggt gtgcgagttg
     6061 cgagcagcga taatcgcggc ctcattggca atattagcca gatccgcccc cgatgaaccg
     6121 gcggctttgg cagccaacgc atcaaggtcg acgtttttgg ccagcggctt cttggcgaaa
     6181 tggaccttga gaatcgcttc tcggtcttta cggtccggca gaccgatgtt aacacggcgg
     6241 tcaaaacggc cgggcctgag taaggcgggg tcgagcacgt ccgctcggtt ggtggcagcc
     6301 agcacgatga cattttgccc ctgttcgaag ccgtccattt ccacgagtat ctggtttaag
     6361 gtctgctcgc gttcgtcgtg tccgccaccc atgcctgaac cacggcggcg gccaacggcg
     6421 tcgatttcgt caacaaagat gatgcagggt gagttcttct tggctttagc aaatagatcc
     6481 cggacgcgcg aggcgccgac accgacaaac atttcgacga attcggatcc ggaaatacta
     6541 aagaacggga cattggcttc accggccacg gcgcgtgcca gcatcgtctt accggtgccg
     6601 ggaggaccaa cgagcaagac gcccttcggt atgcgggctc ccatgccggc gaacttcttc
     6661 gggaacttca aaaactcaac gacctcttcc aagtcttgtt tggcttcgtc actgccggcg
     6721 atgtctttaa aggtcgcttt gtctttttcg ttgccgtata agcgggcacg gctcttgccg
     6781 aaactaaggg cttggttgcc ctgcccctga gcactgcgca acatgaaata cagtaaaccg
     6841 acaatggcta gcgtcggcag cacggtttcg gccaccgaaa cccaggtcga ggtgccactg
     6901 ctcgcggggg tggtgtcgat ttctactttg gagaagttaa agccttcgtc tttgagcgtg
     6961 gcgttgggtt cgagatagga cttgagtgtc gcatgcgttt cgcccttctt agtaatatcg
     7021 agctggttac cggtcaccgt tattttctga tacttgccgg cgttggcctc ggtgacggcc
     7081 gtggttatag ggattttctg taaattgctc ggctgattgg cagctgcgaa gacaatcagg
     7141 ccaaaaagaa tgatcagtgc gatgaagccg gcgtttttca tgccgcggtt attgctgccc
     7201 ggtttgcgcc cagaacgaag ttgttttctg tccatagtcc tctattatac ccctgctaca
     7261 tatttttggc tagcgctcag ccctcacgag tgccaaatca ctactattca cctgcataaa
     7321 cctatcctgt aatacattat accgcgatcc tttcggcgaa actttcgcag ccacgaccaa
     7381 gcgctcgaga gtcttacgat caaagtttcg tacaccgtgc gcccgcagcc actcggccat
     7441 tacttcgcgc gccacagcat ggggtaaatg attgaaccag atccgatcta agcgtcctcc
     7501 cacagattgg aggtgcaatt ggttaacaag taaagtatcg agctcattgt tggtttgatg
     7561 ctgtgccgca ataatcgcat gaagcttatc tcgagcagtc ttgtcgaact tgggcagcaa
     7621 ccggtggcgg atataattcc gcaaataatc caggttttgg ttggtgctat cctcgtgcca
     7681 aacgagtccc tggtctttgg catacgcaat caactcacgc ttcggcacct ggagcagcgg
     7741 ccgttcgata tcatgatggc ttttgagcga agtcagcccc ttccggccgc tactgcgtaa
     7801 aagattaatg atagccgttt ccaaaacgtc gtcctggtga tgagcggtga taatggctcg
     7861 cgcaccgctg gccccgcgga ctttatgcag gaactcatac cgagctttcc gcgcctcggc
     7921 ctcactggtt cctgctccta gatgactctt atgatagaca aaaggcaacc cgtagcgtcc
     7981 tgccgccgct tgtacgagcc gtcggtcttc ggccgaatcc tcacgaatgc catgatcgag
     8041 atgtgccaca attaacttcc acggccggga atttgaaaca gcctcactat agagcatatg
     8101 cagcaaagcc atagagtcca ccccgccgct gacggccaca atgtaggtgc ctggctttgg
     8161 caatacgatg tccatacttt attattgtaa agcaatggcc gagagaagcg acctcttaaa
     8221 acgtattggg aaactggaag cccgcaataa aagagtcgag ggcgataaag cctgggaaac
     8281 aagctgggtg cgtcgatcgt caatcatact gcttacctat ctgacggtcg ccttttattt
     8341 acatttcgtg gtgcatatca gcccctggat caatgcccta gtgccggtta tcggctacac
     8401 gctttctacc ctcaccgtcc agcagctaaa acagcgctgg ctggatcagc agaaatcaaa
     8461 acgcactagc aaacaggggt aaaacttggt aagctggaaa cagcttacaa caaaacaaac
     8521 atgtatcacg acctgatttt atttttgacc ggcattatcg tcggcgccat gaacgccatt
     8581 gctggtggtg gcatgctgat tgggtttccg gtgatgctgg ccctgggtat cccggcgctg
     8641 gtagcaaatg ccacgacgtt cgttattgtc ataccgggca atataagcag catctatggc
     8701 taccgtcagt tactgcacaa agtccctcgg cactatgcct ggctgctcat ccccacagta
     8761 atgggagcct tcatcggtgc tcaagccctc aaacatacct cccagggcgg atttgcgcac
     8821 cttatcccct ggctcattct ttttgcagtt gtgctttttg ctttccaacc atggctctat
     8881 aaacagctcc accagcacct acatgggcca aaacgaaagc gaaacagcct gcggccactg
     8941 tggattaccg gtcttgccct tctcccgata gctacgtacg gcgggttttt tgccactggt
     9001 tttggtttta taatgctggc atttttagga ttcacgggct tgcataacca tattcaccgc
     9061 atgaatgcct taaaaaatat tattggttta tgcctggcca ccacggccct gctctgcctg
     9121 gcgaacgcgc atgtgatcga ctggcggcat ggactcgtta tgggagccgg taatttcatt
     9181 ggcggctact ttgcggccac cagaacccaa aaagtgtctt ctcatgctct gaggataatc
     9241 gtcatcattg ttggcgcctg cgcggccatt tatttgtgcc tccgcaacta ttaacctcta
     9301 gccaacagat acaatccatg gtatatttgt aatctgttag ggcggcgtag ctcagctggt
     9361 tagagcacag gactcataag cctggggtca gtggttcaag tccactcgcc gctaccattt
     9421 cgcttaggct acacactggg gctagtcagt actgaatagg cgtagcctat tttgtctgag
     9481 agtatctaag gataagtcac ctgcaaccgt ctcaggcagt tcgggtttga gcttttcggg
     9541 ctcctcattg tcaccttctt caccttccaa gtttttataa cgagcatcct ccagctcttc
     9601 ataatgtacc aagcaaattt ccggctcccc agccagaagt ccttcaatca gtataagagg
     9661 cttaaacttg tgtcgatagc caaaaactgc cttccatagg tcatccttgc gaggtgtctc
     9721 cgcaatgaat tcagcaactc cataatcaag aataagaata gtgtcatcct gcttgttcac
     9781 cgctcttcgc attgcttcgc ctacattaat ataagtaatt tttgattggg gctgtaagtc
     9841 taatgcctta aatatacgct cgtattgcgt cgcgccattc aaatacaata gcgaagttat
     9901 tgccccgttt tcccaatcca aaatacctgt ctgctttttt aaatctccta ggcttttatt
     9961 caataagaac tccatgaccg gcttgtcata ctgggtgtac tcatcgccaa gctgttcaac
    10021 cattttttca ccctgtctta cgaagtactg gtggagggcc tggtttttgt tgggtacgtt
    10081 ttcagggtca acatttgtga ttgatgagcc cattgtcagg gctgccataa agagcgctgg
    10141 ctcatctatc aaacctgact gctcagcatg cctaacgaag aatcgcaagc tgtcactgcg
    10201 tattgccgct ttcattaggc tgtgcaacgg tttgtcggtt tcgtgcaaag actcatactc
    10261 gtgattcctt gaccaattat tacttagaag cctgtcgcga aactctttaa aaaagcgggc
    10321 aggagccgcc cgaaacaccg tatctttgcg gtcataatgc aaatcgtaat tctcgataga
    10381 aggcatatcc tcttcattta catcttttat gtgctcttcc ccgccgatga atgaacaata
    10441 ggcaccggtt accacttcac aaaaacgggc aaaacttcca taatgttgag tccgcttagg
    10501 ttgctgatag ctcaccagcc ccgtaagaaa agcttctgcc gcggatttat caaaagaacg
    10561 ttcttttaaa tactcagtca gaaagaattc ccgcgcgtag acttgcatag acgtgatgtc
    10621 caaaaaccca ttaatatcat cgtgaggact cggctgccaa ccatccaaca cgtctactag
    10681 agaacgcatc gcccaaacga tatctttgta tatgtcttta tcggtaatag ggtcatcacc
    10741 taagccaagt ggctcgccac ggcagctgtc caagtcaaca aatgagccaa gtgccgtgat
    10801 gttttcactg tgcaggaacc catggctcac tccaagtttg tgcatcttag caacattaat
    10861 gcctaactcg acagcaaact gcgcgacgat gtacgtcatc gggtgaagct cttcaaggta
    10921 ttcgtgtaag ttaggatcgt cattttggcg aattagaaaa tcataaaact catccaacgt
    10981 atcagggtca gccaactctc caaagcgata gggggcatcc attgcacggt aggtaatgag
    11041 ataatcaaca tcgtgaagtt tctcaatcat atccgcttta acttctagtg gcgtgcggct
    11101 ctcttctaat tcttcttgtg cgtaatctgc cgccaaagcc tctaccagct ccggcaaagg
    11161 cacaagctgc ctggtgtctg ggttatcagg gtcagtatgc atggggaatt tatctgggat
    11221 aaccaggccg caaatatact ccgctggtat gccatgctct cgcaatagcc ggctagcacg
    11281 aatcacacgc tctaaaacca aactttcctg taacccgtga ataatgaagc cgcgagacgc
    11341 cgtttccgag ctaaaaatgt gaggatctga aaagtcagaa cctttaattg atacgctttg
    11401 atagtggttc ccaaactggt cttcgacggc cactttaccg gccaagattt cgagtgttct
    11461 tccatgaaag ctggccagac ggccggctgg atagtgaacc tcttcatccc atggaagaac
    11521 atctggaact tcagtttgta gcactaactg gggctgatcg taggcatcat actctgaggt
    11581 taccgcgggg gtattgccaa tgactgcatc atagtcccga acttggccat taggcagagt
    11641 tacctcaaag tgtgcgggct catcaatcag tgtgtcgtta aagccgctga gttgttgggt
    11701 ctcgagccga atcatagtag ctatttcttg gcatctgagc cagtgccact ataagcaggt
    11761 ctggctggct gctgggcagt aggggtgggg ggttcgggtg gttcttgtcg tctgcgcaga
    11821 aagcctcttc gttccgaggt tgcctgtaag gcacggactg acttttgtcc taaggcaata
    11881 accaactgcg attgtttccg gtacgaagat acctgttctt cgcttagtgg aggctccacg
    11941 tgcaaattgc cagccttcac atcatccaag ccctgcacca gcgtctgtgc ggacactagg
    12001 cattcattga catctttaga agggtcttcc gcatgagcca ataggccatc agcaaccctc
    12061 ataaaatctt cttgagaaat aacctgcctt gaatttgctt caaattctcc cattgcgcaa
    12121 ttgactccta tattttgcta actttactac tgttatgctc aaatgtcaaa aatgcgcact
    12181 gtcctgctta tacttgcaaa tcaaagtggc tggtgtttaa tttgttacac attagagaaa
    12241 gttagattta gggtagccta gtttacaact aaaataaatt tagtagagtt gctgattata
    12301 aatatccagc tcaaggtgtc catcatggcc cacatcaggg ttgcctcgat acaccgtaac
    12361 gccgcagttt accggcttgt gattggcgtc taagtctcta aaaccgtctg catccaagcg
    12421 ctccagattt gctcgcagtg acaggataga aaggtggtgc ccgacaatca ttacattctg
    12481 ttcagcataa tctcgcgtta aagctccaag cattgagcgg tgccgttcac ggacatcggg
    12541 cacattttca ccctgcggat agcgatacca gtacgatccc tgcagattgc gtaacgcttc
    12601 ttgctcggga tgcataatat taaaaatgcg ccagtcgtta tagaacaagg ccaggccgtg
    12661 ttcttgctct cgcacgcgct cttcttccac cgtttttaca cttgacagct ccggccaccc
    12721 cacagctaac tttcctaatg tctgatgggt gcgaagataa ggcgaaacaa atatgacatc
    12781 tggcagacct ataagctcgc ttaggcgcga gcccgtaacc tctgcttgtc gctcgcctgt
    12841 ctgtgtaagg cgggtatcat ggtctccgca gcccaaaata aaagtgccgt cttgtatcaa
    12901 ctgttctgcc aggcgtctgg cagtatctgg atccttcttg cgtcgattat gagcccgctt
    12961 aaattcggca taaagaggat caccctcctt gaggccttta agctcgttat aggcagactg
    13021 tccgtgtcgc accaccgtaa gagtattggg ccacttcatc gcaatactac tcgttggcgc
    13081 ccgaaggatc aggtgtcaca aaatcttctt ctattttgta gtatcggatt ggcacgataa
    13141 catatttggg ctgctgaccg tcttcagctt ccttcatcaa ccagctgtca gcctgagagt
    13201 gaatagttcg aggaccgccc ataagccaga aggcgttggc ctgattgacg ccggtagcaa
    13261 ataagttagg ctgtgcatcg gtaaaagttt gaaacgccat gtatgattca tcgtaaggta
    13321 ctaccatgta agtatcctta acggcttctg cgctacgcac cggcatatca agtttgcgct
    13381 cggtatcagt tgtagcaagc ccgtggtggt cagggtataa atccatcaac cggccacgtt
    13441 ccctcctaaa tatcacggtg tatagcaaat cgcccttgtc gttatcatgc caaaaagcta
    13501 atcgccgata gcgaggcctt gcttcattac ccagctgccc ttgctcttct gcgttaagtg
    13561 caatttctac ggcagccttc gcctcatcag atagcgtctc gtaattaacg cccgtttgaa
    13621 cttcataaat ttcgctttcc tgctcagtgc tataaccaag aattgtacga tgcctgtaat
    13681 taatgtgtgg caaaagtatg cggctaccgt cgctcaacga agcgcccagc aactcacctt
    13741 cttcaaaccg ttcagtcagc attggactat cagagtcggc atatagtact tcggattcac
    13801 tgccgaaaat taagggcgtt gcctcttttg ggagttgctg tttaaataat cgtaattgcg
    13861 accagcccaa ggtttgtgtt gcataattgt agacgtgacg gaaattattg tcgctctcta
    13921 cactcctaag ctcctgcatc ttgaccacaa ggttagcggc aaaggcttta tcttggccct
    13981 ctgctcccga tatgatgctg ccaaaaccta tagccaaacc aactttgttg gcatctaaga
    14041 acgtgttgct gatattgacc agctgttgat taagcctgtt ggtgcgcgaa tgatgctctt
    14101 tagtcggtgc attctgcatc acatatttga tagaaatcca tagtgcttcg gctaagtcca
    14161 tataaaaact ggcgtcttgt gtgttaggct ggcccgtctc tcggtttact ggtttagtca
    14221 aatctccttt aaggtaagct tcgtaagcag ccagctggtc cagaaaatac tcccaagttt
    14281 cctcaggcga caaagcctgc gcggcttcaa cgacaccggc tatgtcagcc tctgaccctt
    14341 tttcatctag ccgcgcttgc ataacggcat agtagcttgc ttgtctgcca atccgcgctt
    14401 ttggcattcg gtgtcctact acggcggtag caaactgatt ttctagcgcc acccagtatt
    14461 taagcccagt ttggctttgc tccgttacgg attcgtcgtt cttataatat ccatgaagaa
    14521 tttccgttgc ctgattacct acaaaataat aggcttcgtc cggtctgttt aagaatgagt
    14581 tcctatgata aagggtgtct cgcatctcct gtgctggtga ttgctcttgc cgaaatgcgc
    14641 tttcctgttt agtcatactt acctccgttg tctatcaata atgtaccaga gaaataacag
    14701 atgcaagcat gagcaagtta cgatccgcgc atcttttcca aatcgtcttg tagatgaact
    14761 cttacccggc agattgaacg agcttgcgat agagcgtgaa tagagcagtc aggcaagcca
    14821 agtaaaaaag aacgcttagg cagacactca tgaggccggt gcggtactta atagctaatg
    14881 ccgttctggt cgttagcaag ccgccaaaac ttaagccaga caagcgcgcc aaattatcat
    14941 aattaccgcc tctgtgtcgc caggaaccaa tgaccgcacc agccataaat gccagactag
    15001 cgaaagcgca tgtcaggtga attataagta acatagcctt actatagctt aaagacccgt
    15061 ctcccttcta agtcatttaa gcactccacc ttcttgcagc agaatcgaga gataataggg
    15121 aatccgctct ccgtcggcta atatagctgt atggctatac agcaggcagg gcaagagcac
    15181 cttacaactt tgactgccca gtgggagttg accgttttgg gccacagcag tacccaggat
    15241 gcgtcttttg tcataaacca caccaagcct gaggtgctgg tggcgacagg atttggcgaa
    15301 accgatattt ctcaagtggc ggagcggctt ggccatctcg gaataagggc cgctgtgttc
    15361 aaccttgctt atccgcaaac cgttggcggc gaggatttaa agctgacgcc ggcaattatg
    15421 gagcaaacgg tgcttaaaag cgtgtccgca atcgcgcaag aaataaatat gttcatgggt
    15481 tacgacgcgc aaacgcctat gagcatggtt ggtcattcaa aaggttttgg cgaattattg
    15541 aaatttgtta gcgcctatcc tgaagcttgc gataacctgg caggtgcggc acccttggga
    15601 gcaaactgta taccgaaaat tatgggctct acgccgaaag agtatcgcgc caattttaaa
    15661 acacgtctac gggagtctgg cctactctcc tcattggttt cacacccaga ggccggccgc
    15721 ctaggcaatg agttcatggc aggcatacag tatttgcacc aggaagatgc accggctttg
    15781 atacgaaatt tgcacggtat gggtattcta aaatccatcg tcataggctc tgacgacgca
    15841 ctttgcccac cagcggaatg cgagcaggca ataggtattg caggcctcat tgttaccacc
    15901 gcgggcaaac atgaccacat ttccacgccc gagggtatgg cccagattgc tctggctatc
    15961 gagcagtgca tcaatcttga aacacgatga agctcaagca cgtcaaggga ataatctggg
    16021 atttagatgg aacgctcata gatagcttcg agatatttga gcaaatcata gccgatgtcg
    16081 tcgagaccag cgggcacagt atgccgacac acgaatacat gctcagcaac taccatggct
    16141 ctcttgaaga aaccatccag aaaatactcg gcctggaatc ggcagccgaa gtcgacgaaa
    16201 ttataagtat cttcgccgaa aagcaggaac atcactatgc cggcgacctg gagacgcatt
    16261 tgttccaaga tgcggccatg ctggcccaac aagctgccaa gctgggtatg caacagctga
    16321 tcgttaccaa cagagaccac gaaggccgcg gtacaggtag cccgcgagcc ataattgccg
    16381 ctaccactct cgccgactgt attcacgaaa ttcgctcggg agatgaagtg gacttccgca
    16441 agcccgataa gcgctctgtg ggcgattgga tggagcgcca tcaactcacg ccagccgaag
    16501 tcgtagtgat aggtgatcaa tttgtcgacg ctcaattagc attaaacatc ggagcccgag
    16561 cagtcctgat caagcggaat ggcgatatac cgcaccttga tacactcgaa gatcacgagg
    16621 atgtggttat cgtggacagc ttagagattg tagaactagt tcgctagcac cgccggccat
    16681 tgctgttaaa atatagatag tgacagccaa tgtgactact cgaatttcta tcgccgcatc
    16741 cgccagtgaa gtcttcgaat atctcacaaa taaaaaaata ttacctccta tggaatcccc
    16801 aaattactaa tctctccggc cacgggaagc tcaagctcaa cagcacctat tccacaacca
    16861 gtaacgtgct aggcgtcacc atcaagtcca aaaatgtcat taccaaattc cgagccgaca
    16921 aactgataca gattgagaat aaaaccggcc tggtgcacta cattgcccgt ttcaccctaa
    16981 agcctgaccg aaagaaaaca ctggtaattt gtgctaccac cgtgtcttcc gaaagctctg
    17041 cttttgcttt tgcagtaccg gttttgaaac ttctggctcg gcgagaattg caaactgact
    17101 tgcaagccct aaaattagcc tgcgagcacc agctgctgca ataaacagat ggggcggcta
    17161 gataacttaa agatagactg cctgcccgac agcaagcttg ccgatggcgg tttgcacctg
    17221 ttgcatgatc cgcgataggc cttgcgtcaa aggcacattg aacttgcgct cgtgcgtgcc
    17281 ttcgtcggtg ttttcgggca ttggctgttg cagaacaaca taattgcctg agcgaactaa
    17341 tctaataaac tcggccactt tcgcctctgc atcctcaatc tcgaggtagg tgagcaatgt
    17401 gtcgtaccat tcctcaagca caatttcgat ttgggctgct tcttcgctct ctgctcgatc
    17461 agtcatgatt aacacctgta gccgattagt cgctagcgcc atctgtacca ctaattcgct
    17521 gacagcttcc gcaacctccg gctcgctctc ttgaacaaat tcagctagtg aaatttgcac
    17581 cacttccggc agatgactca gcaagacttc ggttttttca gctacggatt ccggtggggc
    17641 tataacttcc gcaatcttcg tgaggactat cgtcctcgat tcagatagtg cttcaggagt
    17701 tacagcctct tgattttctg cctcagacat tattcctgga ataattaatg tatcgatcca
    17761 gctatcatcc tcggtttcat ctgaagctag ccccaattca gtagcaaaat ctatggcttc
    17821 agcctcgagc gacaccgatc gagcttcgtc attctcaaac gcaaaagatg cttcgctccc
    17881 cgcagcctca agctgacttt caggtacccc gtttacagct tcatgcgccc ttacgatctc
    17941 cggtgagacc gacttgagcg acggtttagg cacaacttgc gtgacgaaat ccggcttatg
    18001 ttctgtggag gcaatttggg ctggcttgcg cggctgctct ttggggccac tcttttgagt
    18061 gggagtaatg aacggcctgg gcgtgaggcg gttgactata gatggcttag tgggaagttt
    18121 ttgaacagac tcaattggtg actcaactat tttttgcgtt tccgccttat cttctttacg
    18181 gaaagtttcc tgttccacgg ccgcttgttg ctggcgtata ttttgctcgc tcaaagtaac
    18241 tactaactcc cgttcagtgg gtgggcgtag atttttcgcc tgaggcttta tttctatttt
    18301 ggccgatggg gtcttagcta ggatctcgga ttttttgatg gtttgaggcg ggcgctcagt
    18361 ttcaacctgc ttttcgtgct ctacaggctt agcctctggt tcaactactt tttttttggg
    18421 ttttcacgag cgagcacttg ctcgcttact acgatattaa gccccggctc tcttttgctg
    18481 agatcttgat acatgtcttg cacttcagca agtccacctt ttttataagc attacgtgtc
    18541 cattttccca tagggcatga gtcgtgtgaa agcatagtag caacagtggc agtctgtacg
    18601 ccttctgttg tttcgtaggt tacatattgt gctagttcag ctaccccaaa accggtttcg
    18661 ttctgaaatt gttccaggct cgtcttggcg aatacacctt ccagttcagc tcgagtttgc
    18721 ggatcaaggt ccgctattga ttgaggctct tgggctggcg aggataaacc ttcatatagc
    18781 attagctaca ttgtagccat aagttttaac ggaatccatc gctaagtcga atatttaagg
    18841 attatttaag atggttacag agatggacgc ctttgatgta aatattgttt gcacatttta
    18901 ttgatgattt taggaggcgg cgtactattg gatccggagg ctggcggcaa aaaataaaag
    18961 gagggtccgg tatggggcgc aaggtgtata ttttattggt tttgattcta gggttagcgt
    19021 tttctgcagg tggcggcaga gcatcggcag ctgttcattc tcgtaaagtt tgctctatta
    19081 gccacgcagc cgggactgcg cgctgtcatg ccatcatttt gactgacggc agtaatgtaa
    19141 caccacttgc caccacagcc ccacaaggct atgggccggc cactttcaaa gctgcttacg
    19201 gaaatagtgg tagatcaact acccatgtcg ccgtcgttgt cgcctatgac gcaccgacga
    19261 tagccagtga tcttgctatc tttagccgga cctttggctt gccggtttta ccgacttgta
    19321 cttcggccgc tcaaagcagt tgcttcgaga agcttaacca gcgtggcagc tcgagttatc
    19381 ccgcagttaa tagcagctgg gcagtggaag ccagcatgga tgtcgagtcg gtgcatggcc
    19441 aatgtcccgg ctgccggatc agtcttgtcg aagccaattc agcaagcatg gcgaatttgt
    19501 ctgctgctgt cgatgaagct gtggccaccg gtgccaaagt cacctccaac tcctatggtg
    19561 gcgccgaatc cagtggtgaa gccgcctacg accgccacta tcatcaagcc ggcgtaacga
    19621 tggttgtcag ctccggcgat agcggctacg gaaccgatta tccggctgct tcgccatacg
    19681 ttatcgccgt cggtggcact agcttaaaaa tgaatggctc gaaggttgcc agcgagatag
    19741 cctgggatgg cgccggtagc ggctgcagcc ttttcgaaaa caagccatct tggcaacacg
    19801 ataaaaaatg tgtgatgcgc agtatagccg atgtagccgc tgatgctaat ccaagcactg
    19861 gcgcggcaat ttatgacagt tatggcagcg gcggtcatca tggctggctt acggtcggcg
    19921 ggactagctt ggcagcaccg ctggtgacgg gcattatcgc cgcctccggt aatagcagca
    19981 atgtatccat cctcctatac ggccacacaa ctaacgtcat tcgtgatatc gtgggcggca
    20041 gcaatggtac ctgcgtgagc tacctttgca aagcccttgc cgggtatgac ggtccgacgg
    20101 ggctcggtgt gttaaatcat ttataaaact agaactccgg gagaggagac ggtatgatgg
    20161 tacgagtaga ggagttttat gcgcttcaac aaacaactat tcaagaatgg ctggctgcta
    20221 tttgcctgcg gcctcgcact cggagtcctt gtcattttgg ccatacggtt tttcacttac
    20281 aagcccgtgc aggtacacta ccatgccaat tttgcagttt acctaaatgg acaacgccaa
    20341 gaattcaaag ggccgcaata ctatcaagag gtagctattt gtagctctac gaatgacatt
    20401 accatacccc agcaacgcgc tcacatgcac gacaacataa actccgtggt gcacgtacac
    20461 gaccatgcag ttacgtgggg gcagtttttt gaaaacctcg gctggtatgt cgggcctaac
    20521 ttcattgaaa cagatagtgg cacgctctat actgagaacg atcaagacaa attacatatc
    20581 ctaataaacg gccaggacta tactgaccta acgccaatca ccaatatgct catcaaggat
    20641 aaatcacgct tactgataag ttttggcgac attagcgaca gcaccttgca gcaagagtac
    20701 aaaacagtgc cgagcaccgc cgcccgcttc gacgctaccc aagacccttc ctcgtgttcc
    20761 ggcaccgaaa aagtaactgt aaaagaccgc tttcaccatc tgttttaagc tatgcatatt
    20821 ggcatcattc tcctcgtttt cgccgtcatc ttcatcgctg agctgcccga caaatcgttg
    20881 tttgcttccc tgattttagg cagcaaattt cccgccctct atgtctggct tggttgtgcg
    20941 gctgctttcc tcgtacatgt catcatcgca gttctcgccg ggcagctctt gaccttgctg
    21001 cctcacctag tcttacacat catcgtcggc atcctttttc tcgctggcgc gctactgatc
    21061 tttttcggca aacacgggtt ggaagaggcg acacaccatg agccgaaacc acgaaaggtc
    21121 gtggcgccga atttctggaa agtattcggc acctcattta ccatcatctt cctgggcgag
    21181 tggggcgata tcacccagat cagcgtcgcc aactatacgg cacactacca cgacagcctc
    21241 aacgtgggca tcggggcgac actcggttta tgggccgcgt gtgggctggc ggtgacagcc
    21301 ggatcaaagg ttatttcgcg cgtgcctgcc aaaatcctgc agcgcacgac cggcgttatt
    21361 ttaatcctgt ttgcgatcat cagtttcgcg acagctgtcc agaaatagac agcgttttta
    21421 tacttacaaa tcgctgcaaa gggtatatta tgagagcaaa taattagaaa ggctctcatg
    21481 aaaacagccc tcggaatcct tgtcgtcgta ctcatcctga tcggcggcta tgcagtcgtt
    21541 aagcataatg acaaaaacgc ccctaacacc tcgacttcat caaatagtgg cggcaattcc
    21601 agcaacatga ccatgtcatc gaaccagagt tctagctccg ccagccaaac caacgccgtc
    21661 aatatcacga actttgcttt cagtccgagc aatataaccg tcaaaaaggg caccgccgtc
    21721 acctggacca acaaagactc agtagcccat acagtgaccg aaaacgatgg ccaagacggg
    21781 ccaaaaagca gcaacctcga ccagaatcaa acctatagct tcacttacaa tacggtgggt
    21841 actttcaaat accattgctc tatccatcct gacatgaccg gtagcgtcac ggtcacaga
//
LOCUS       DATNGP010000016         3866 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_3010433, whole genome shotgun sequence.
ACCESSION   DATNGP010000016 DATNGP010000000
VERSION     DATNGP010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3866)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3866)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3866
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3010433"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..435)
                     /locus_tag="VN778_00400"
     CDS             complement(<1..435)
                     /locus_tag="VN778_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015802424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HXR49476.1"
                     /translation="MTKITAVCFDVGETIVNETREYGTWADWLGVPRHTFSAVFGAVL
                     ARGGDYRETFQHFAPGFDLTAERQKRIAAGKPETFAEEDLYDDARDCLAALQKQGLHV
                     ALAGNQTARAEGILRSLDLPVDTIGTSDSWGVEKPSAAFFDRI"
     gene            528..2330
                     /locus_tag="VN778_00405"
     CDS             528..2330
                     /locus_tag="VN778_00405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49477.1"
                     /translation="MTTQTPNQAPPGGDFEEPPIDPRVLSEAYATGGEAGIREAILAH
                     KIGAVVRAAYAAGGEKGVAETIQRRTEDAYARHGERAFADGAAAWFDIAPSNGSNGHA
                     SARPQPGTAGQPRQEARQRPPETGQPAGAGRAAPGPDSSPQSSQRPSAPGRPQAERTR
                     QQPPRPPRQEETRRPEGRPADREQQTRQPRQAPAPHAAHVHGRFQGTHTTVRDKVNGA
                     RGPAWAPEPGRPRHTEVFDAQADIQRRRAKGRQGPEYPRFYQPRVVRPEVFKQWNTET
                     TIDDSEMRCVGKNEAEDIFERAELRGDRKFFAATQDLLKEAGLLPHHRLIVEEDRAIW
                     FSKAFDIDDRGRAAIIGYVETDDGKIVARSYYRSQSSGAWRYLPQYRYNLDDERVDWY
                     GKGYGEESLTLPAVLQEGLTHAVDADGGLIRLDEKTREAAFYGTTRDIKSEGTVYYRS
                     VSRAPRDIRGMPHKDDKRAPEDLIISLEHAPRFDKRPMRSWTMDTSLYGRVECEVFHS
                     ENGQLGYSFCRDKRGRAWISSIEDLASHVESTGLRTEWISSVLTTPAYEYSTQARGYG
                     NDNDRDGHYVDMFENYLSKIPVIKDYVRSKSQRR"
     gene            2376..2819
                     /locus_tag="VN778_00410"
     CDS             2376..2819
                     /locus_tag="VN778_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020620670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXR49478.1"
                     /translation="MKPRVNVITLAVTDLNKSLVFYRDGLGLPSRGILGEEFTGSETE
                     PSGTVAFFELQGGLIFALYPRTELAKDAKKPVGTPNSTEFSLGYAVNSKEEVDELLQK
                     AEAAGGVLTDKPHERPWGIYSGYFADLDGHLWEVIYNPGLAVEDL"
     gene            complement(2984..3316)
                     /locus_tag="VN778_00415"
     CDS             complement(2984..3316)
                     /locus_tag="VN778_00415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49479.1"
                     /translation="MEGTAMLYIMLYVLSLTTCNTVAAWRRQDTDYDLIVTIRAILVI
                     ASIAWAFIAFPTKPTAFQNGIIALGVIAALLLFADKRVKGTSQRLPYVLIAGGNLVAL
                     GLCAVALM"
     gene            3568..>3866
                     /locus_tag="VN778_00420"
     CDS             3568..>3866
                     /locus_tag="VN778_00420"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HXR49480.1"
                     /translation="MIKVAVLWLISEDDEVLLAQRALNKKHDPGVWGPAVTGKLESGE
                     TFDEALVREVEEELGLTTKDYAPRFLFETDYNHPDGEVRKFGVYVATLPKAKTGL"
BASE COUNT          986 a          880 c         1134 g          866 t
ORIGIN      
        1 gatccggtcg aaaaaggcgg cggaaggctt ctcgacgccc cagctatcag aggtgccaat
       61 ggtgtctacg ggaaggtcta gtgatcgcaa gatgccttcg gcgcgtgctg tctggttgcc
      121 ggctaatgcc acatgaagcc cttgtttttg tagagctgct aggcagtcgc gcgcatcatc
      181 atacagatct tcttcggcga atgtttctgg tttgcctgct gctatgcgtt tttgtcgttc
      241 ggccgttagg tcaaagcccg gagcaaagtg ttggaacgtc tcacgataat caccaccacg
      301 ggcaaggact gcgccaaaga ctgccgaaaa tgtatggcgt ggtacaccca gccaatcggc
      361 ccaggtgccg tattcacggg tctcgtttac gatggtctct cctacgtcaa agcatactgc
      421 ggtaattttt gtcatgcagc tagtatacat gtaaatccgt agtggctgct tgtgtggtcg
      481 gtgggctgac tatatactga caactataag cgtgaatgta actaccgatg acaacacaga
      541 ctcctaacca agcgccaccc ggtggtgatt ttgaagaacc acctattgat ccacgggtgc
      601 tgagtgaagc atatgcgact ggcggcgaag cgggaatacg tgaagcgatt ctggctcata
      661 aaattggcgc agtagtgcga gcggcgtatg cggctggtgg cgaaaaaggc gttgccgaaa
      721 cgatacagcg cagaactgag gacgcatatg ctaggcatgg ggaacgggca ttcgcggacg
      781 gtgcggcggc ctggtttgat attgcgccct caaatggttc gaatggacat gcgtctgctc
      841 ggccacagcc aggaacagcg ggtcaacctc gtcaagaggc taggcagcgt ccacctgaaa
      901 ctggtcagcc ggctggggcg ggaagagcag ctccgggtcc tgacagttca ccccagtctt
      961 cacagagacc gtctgcaccg ggcagacctc aggctgaaag aactagacag cagccacccc
     1021 gaccacccag acaggaagaa actaggcgtc cggaaggccg cccagctgac cgggagcaac
     1081 aaacacggca gccacggcaa gccccagccc cacatgctgc acatgtgcat ggtcgattcc
     1141 agggaactca cacaacagta cgcgataaag tgaatggtgc acgagggcct gcctgggcac
     1201 cagaaccagg taggccaagg catacggaag tctttgatgc tcaagcagat atccaacggc
     1261 gccgtgccaa aggacggcag ggacccgaat atccccgttt ttatcagcct agggttgtcc
     1321 ggcccgaagt ctttaagcag tggaacacag aaactaccat agacgacagt gaaatgcggt
     1381 gtgtcggcaa gaacgaagca gaagatatct ttgaacgggc cgagctgcgc ggcgatcgta
     1441 aattttttgc tgcgactcag gacttactaa aggaggcggg cttgctgcca catcaccgtt
     1501 taattgtgga agaagatcgg gcgatttggt tttcaaaagc ctttgacatt gatgatagag
     1561 gtagagcggc gattattggc tacgtcgaaa cagatgatgg caagattgtt gctcgctcgt
     1621 actatcgcag tcaatcttca ggagcttggc gctacttgcc acagtatcga tacaatctcg
     1681 atgatgaacg ggttgattgg tatggcaagg ggtacggtga ggaaagcctg accttgccag
     1741 ctgtcttaca ggaaggatta acgcatgctg ttgatgctga tgggggcttg ataagactcg
     1801 acgagaagac aagggaagcg gcattttatg ggacaacccg agacattaag agcgagggca
     1861 cagtttatta tcgaagcgtc tcaagagcac ctcgggatat taggggtatg ccgcataaag
     1921 atgacaagcg tgcgccagag gatcttatta tttctcttga gcatgctcca agatttgata
     1981 agcggccgat gcgctcctgg acgatggaca ccagtctgta cggacgggta gaatgcgagg
     2041 tattccattc tgaaaatggc cagctaggat atagcttttg ccgcgataaa agaggccgcg
     2101 cctggattag cagtatcgag gacttggcaa gccatgtgga atcgactgga ctgcggacag
     2161 agtggatcag cagtgtcctg acgaccccag cgtacgagta tagtacgcaa gctagaggct
     2221 atggtaacga caatgacagg gacggtcatt acgtagacat gtttgaaaac tatcttagca
     2281 agatccctgt tattaaggat tacgttagaa gtaagtcgca gcgtcgttag gatgcttcga
     2341 gatatgccgt aggctaattg ttgtaaggga tacttatgaa acctcgtgta aatgtaataa
     2401 cactagctgt aactgatctg aataagtctt tagtttttta tcgtgatggc ctaggactac
     2461 cttcgcgggg aatccttggt gaagaattta cgggtagcga gaccgaacca tctggcacgg
     2521 tcgccttttt tgagctgcaa ggtggattga tctttgcgct ctatccgcga acggagctgg
     2581 caaaagatgc taaaaaaccg gttggtacgc cgaatagtac cgagtttagc ttgggttatg
     2641 cggttaattc taaagaggaa gtggatgagc tgctgcaaaa agccgaggct gccggaggtg
     2701 tactgactga taagccgcat gagcggcctt ggggcattta ttctggatac tttgctgatt
     2761 tggatgggca tttgtgggag gtcatttata atccgggact tgctgtagag gacttgtaat
     2821 aaaggtgtag ccgacggtgt gtctaatgca gtagtaaggg aaaacaaaca gacagaacaa
     2881 catagggaat aacaacaaaa gatacaggta cacgagtgcg cctggtaaac tttttagtgt
     2941 atgcgccctg tgctcttctc tctgttggtt cccccgggcc gtgctacatg agggcgacgg
     3001 cgcagaggcc cagggccacc aggttcccgc ccgcgatcaa gacgtacggc agccgctggc
     3061 tagtcccctt gactcgcttg tccgcgaaga gcaggagcgc ggcgatcaca ccgagggcga
     3121 tgatgccgtt ctgaaaagcg gtgggcttgg tcgggaacgc gatgaacgcc catgcgatgc
     3181 tcgcgatgac cagaatggcc ctgatcgtga cgatgaggtc gtagtcggtg tcctgacggc
     3241 gccaggcggc aaccgtgttg cacgtcgtga ggctcaggac gtagagcatg atgtagagca
     3301 tggcggtacc ttccatagag ttgatggatc cgaatgtatg agatggagtt gctgcctagt
     3361 aaacaaaaga tcttttatac tagcgcacta atcccatctc aaatgttctg tctgattgtg
     3421 aaggtacttc ggagatgata tacctcgacg aagctaatat attatagcac tttattataa
     3481 atacttcaaa ttatcccaat caagcacaca aggttggctc ggcggtggcc tgtttgctat
     3541 gctgggagta aattggaggg ctaaacaatg attaaggttg cggtattgtg gctgattagt
     3601 gaagacgatg aggttttgct ggcgcagcgg gcactgaata agaaacacga cccgggcgtg
     3661 tggggcccag cggtaaccgg taagctggaa tcgggcgaaa cgtttgatga agcgctagtg
     3721 cgcgaagtgg aagaagagct tggtctaaca accaaagact atgcgccgcg tttcctgttt
     3781 gagacagact acaaccatcc ggacggcgaa gtgcgcaagt ttggagtgta cgtggcaaca
     3841 ctacctaaag ctaaaacagg gctgat
//
LOCUS       DATNGP010000017         3645 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_424734, whole genome shotgun sequence.
ACCESSION   DATNGP010000017 DATNGP010000000
VERSION     DATNGP010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3645
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_424734"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            169..1584
                     /gene="zwf"
                     /locus_tag="VN778_00425"
     CDS             169..1584
                     /gene="zwf"
                     /locus_tag="VN778_00425"
                     /inference="COORDINATES: protein motif:HMM:TIGR00871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HXR49481.1"
                     /translation="MTNTPSPLSPSIIVIFGITGDLSQRYLLPALYHLMKDGLLHEQT
                     QIVGISRRDISTKDLFAKVELCVNEVDKVCDPAILGKLHKQTRMVQLDLTDPAGYTRL
                     RKTLDSIETEQGMCMNRLYYLSIPPSIYGSIITLMGQADLNTSCQHKVAETRLLIEKP
                     FGYDLHSAKDLIAETAKVFKEDQIFRIDHYLAKETVQNILTFRFRNPIFEAVWNHEHI
                     ARIEISASEKIGIEGRAVFYEPLGALRDFIQSHLLQLLAVVTMDQPAALDSEHIHVSK
                     QAVLEQAKAVPAGEVSKYTVRGQYEGYRAEVKDGNSTTETFAGVKVFIDSPRWRDVPI
                     TLWTGKGLVEKRTEIVFCFKSEGDQAANRLRFRIQPNEGIELDLLTKKPGFETELQTT
                     AMDFSYDQNFGHQAHANAYERVLVDAIRGDHTLFATSEEVLEAWRIVQPVLDSWSKQA
                     DNLKTYPMGTSGDKLIESLTS"
     gene            complement(1577..2566)
                     /locus_tag="VN778_00430"
     CDS             complement(1577..2566)
                     /locus_tag="VN778_00430"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HXR49482.1"
                     /translation="MDTSSSETLPSLIEQSCKVIAEFQQPSGAYPASPNFDVYHYSWF
                     RDGAFIADGMSRAGQIDSAERFFAWCSEIVVQRRDHILNGGKLDARYTYEGKESTDEW
                     ETFQLDGYGTWLWAMQGHAARHGRLLDPYQEAAGLTQHYLATHWQEPCADWWEERQGT
                     HAASLACMYAGLQAYEHPEAAAVRAAIDMSSERTDSSLLVCPLFNAVDDEAFAPVLDH
                     IESELESEAGGVYRYREDDYYGGGEWPVLTSMLGWYYLKLGRTDEARDKLSWVKAHIG
                     ANGWLPEQSQDQLLHPENYEPWVKRWGKPANPLLWSHAMFLTLATGLQAGETN"
     gene            2646..>3645
                     /locus_tag="VN778_00435"
     CDS             2646..>3645
                     /locus_tag="VN778_00435"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF033740.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarP family serine protease"
                     /protein_id="HXR49483.1"
                     /translation="MIIDLLIILFAISALYRGREIGFVRQLCSTVGFFGGLFLGALLE
                     PHLVGLAHTAAGRSLVTLLTMLGCALVLLTIGEYIGIRLKHKVLLKRVNRLDNGLGAV
                     LNIVSLLFSIWLTAAIIGALPFPSLQATLHSSRIVRGLDRLLPSAPTIIADLGHLIDP
                     NGFPQVFIGSEPSPRSGINLPSLGELQAAVDKDKYSVVKVEGQGCGGIVEGSGFIVGN
                     GLVATNAHVVAGIRSPYVEDSNGTHRAVPIWFDPNLDFAVLRVSNLAGHPLILASSQA
                     SSGTPGAVLGYPGGGPLSAGPAAVLDEFTASGRNIYGRGNTNREVYEVQATVIPGNS"
BASE COUNT          859 a         1015 c          937 g          834 t
ORIGIN      
        1 cggcgaagcc cgctggacgc tggaaacggc acgcgacctt ggcatcccca tgcccagcat
       61 tcaagccgcc ttcgacgtgc gcctggcttc ccagaaaggc gaggttaact ttgcgaccaa
      121 attgttagcc gctatgcgca ataaatttgg tggccaccaa ttaaacaaat gaccaatacg
      181 ccatcccccc tcagccccag catcattgtc attttcggca tcactggtga cctttcgcaa
      241 cgctacctgt tgccagcgct gtatcatctg atgaaagacg gcctgctaca cgagcagacc
      301 cagatcgtcg gcatcagccg ccgggatatc tcaaccaaag acttatttgc caaagtggag
      361 ctgtgtgtca atgaagttga taaggtgtgt gatccggcga tattaggcaa gctgcataaa
      421 caaacgcgca tggtgcaact agacctaact gaccctgcag gctatacacg cttgcgaaaa
      481 actctcgata gcatcgaaac agagcagggc atgtgcatga accgcctcta ctatttgtcg
      541 atacctccct ccatttacgg ctcgatcatc acactcatgg gacaagccga tctcaatacc
      601 agctgccagc ataaggtcgc cgagacccgt ttgctcatcg aaaaaccttt tggttatgac
      661 ttgcattccg caaaagactt gatcgccgaa actgccaaag ttttcaaaga agatcaaata
      721 tttcgtatcg accattatct agccaaggaa actgtacaga acatcttaac gttccgcttt
      781 cgcaatccga ttttcgaggc cgtgtggaat cacgagcata tcgcccgcat cgagatcagt
      841 gccagcgaaa aaattggcat cgagggacga gccgtttttt atgagccgct cggggcgctg
      901 cgcgatttca tccagagcca tttgctgcaa ctgctagccg tggtgacaat ggatcagccg
      961 gccgctttgg atagcgaaca tatccatgtc agtaaacagg cggtactgga acaagcgaag
     1021 gctgtgccag ccggcgaggt atcaaaatac actgtccgcg gacagtacga aggctatcgg
     1081 gccgaggtta aagacggcaa ttccacaaca gagacattcg ccggcgtcaa agtattcatt
     1141 gatagtccgc ggtggcgaga tgtgccaatc acgctctgga cgggcaaagg tcttgtcgag
     1201 aaaagaaccg agattgtttt ctgcttcaag agcgaaggcg accaagcagc caaccgcttg
     1261 cgcttccgca tccagccgaa tgaaggcatc gagctggatc tgttaaccaa aaaacccggc
     1321 ttcgaaaccg agctccaaac cacagccatg gatttctcgt acgaccaaaa tttcggtcat
     1381 caagctcacg ctaatgctta cgaacgtgtg ttagttgacg ccatccgtgg tgatcacact
     1441 ttatttgcaa caagcgaaga agttctggaa gcctggcgta ttgtgcagcc cgttctcgac
     1501 agctggagca agcaagccga caacctcaaa acatacccta tgggcacttc gggcgacaaa
     1561 ctaatcgagt ctcttactag ttagtttcgc cagcctgaag gccggttgcc agagtcaaga
     1621 acatagcatg tgaccagagt aaaggattgg ccggtttgcc ccagcgctta acccagggct
     1681 cataattttc ggggtgcagc aattgatctt gtgactgctc cggcagccag ccgttcgctc
     1741 cgatatgggc tttaacccag ctaagcttat ccctagcctc gtcagtgcgt ccgagcttca
     1801 aatagtacca gccaagcatg ctggttagta ccggccattc gccgccgcca tagtaatcat
     1861 cttcgcggta acggtacacg ccgccagctt cactttccag ctccgactct atatggtcca
     1921 ggacgggcgc aaatgcctca tcgtcgacgg cattaaaaag cgggcatacc aataagctgc
     1981 tatcggttcg ctcactactc atatcgatcg ctgcgcgcac agctgctgct tccggatgct
     2041 catatgcttg caagccggca tacatacagg ccaagctggc ggcatgcgtg ccttggcgct
     2101 cctcccacca atcagcgcac ggttcttgcc agtgggtcgc cagatagtgc tgcgtcaggc
     2161 cagcagcttc ctgataggga tctagtaaac gtccatgccg tgccgcatgg ccttgcatgg
     2221 cccacagcca cgtgccgtaa ccgtcaagtt gaaacgtctc ccactcatct gtcgactctt
     2281 tgccctcata cgtgtagcgg gcatccagct tcccgccgtt gagaatatga tcgcgacgct
     2341 gtacgacgat ctctgagcac caggcgaaaa accgttcggc tgaatcaatc tgtccggccc
     2401 ggctcatgcc atcggcaata aaagcgccat ctctgaacca agaatagtgg taaacgtcaa
     2461 aattcgggga cgccggataa gctccagagg gctgctggaa ttctgcaatg accttacagc
     2521 tttgttcgat taaggaggga agtgtttctg agctactggt atccatatca ggccttccgt
     2581 ttcatgatag caaaacacag ggtaaacacg agcataaagc tcggcgaatt tgctacactt
     2641 agtccgtgat tatagacctg ctcatcatcc ttttcgccat cagcgcgctt tataggggcc
     2701 gcgaaatcgg ttttgtgcgg caactctgct cgacggttgg tttttttggc gggctattct
     2761 tgggagctct gttggaaccg cacttggtgg ggctagcgca tacggctgcc ggtcgatcat
     2821 tggtgacatt actgacaatg cttggttgcg cgctggtgtt attgacaatc ggggaataca
     2881 tcgggattag actaaaacac aaggtattat tgaaacgcgt caaccgcctg gataatgggc
     2941 tcggggcagt ccttaatatc gtttcgctgc tgtttagcat ttggctgacg gctgccatta
     3001 ttggggcgct gccattcccg tcattgcaag caacactgca tagttcacgt attgtgcgcg
     3061 ggcttgatcg tctgttgccg tcggcgccaa caattatcgc cgatctgggg cacctgattg
     3121 atccaaatgg cttcccgcaa gttttcatcg gtagcgagcc cagcccccgc agcggcatta
     3181 atttgccttc gcttggtgag ttgcaagcgg cagtcgacaa agacaagtac tcggtcgtta
     3241 aagttgaggg gcagggctgt ggcggtatcg tggaaggctc cggttttatc gttggcaacg
     3301 gtttggtagc cacgaatgca cacgtggtag ccggcatccg atccccctat gtggaagaca
     3361 gtaacggcac ccaccgcgct gttcccatct ggtttgaccc taacctcgac ttcgctgtgc
     3421 tccgcgtatc aaacctggct ggtcatccgc taattcttgc cagcagccaa gccagttcgg
     3481 gaactcccgg ggccgtattg ggctacccgg gcggtggacc gctctcggcc ggccccgcag
     3541 ccgtcctaga cgagtttacc gccagtggtc gcaatatcta cggacggggc aataccaacc
     3601 gcgaagtcta cgaggtgcag gctactgtca tccccgggaa ttcgg
//
LOCUS       DATNGP010000018         3828 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_3505087, whole genome shotgun sequence.
ACCESSION   DATNGP010000018 DATNGP010000000
VERSION     DATNGP010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3828
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3505087"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="VN778_00440"
     CDS             <1..366
                     /locus_tag="VN778_00440"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HXR49484.1"
                     /translation="AGAEARAESALKQLQHGTDFGTLATQVSEDPATKTNGGQYGAPI
                     TQSDRDIAPAVTTELFKLKPGQISGIINTGYTLEIVKVIDRSSDSLHAAHIQFNLQDI
                     TTYTKPLAAKEPAHRYIKV"
     gene            426..502
                     /locus_tag="VN778_00445"
     tRNA            426..502
                     /locus_tag="VN778_00445"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:460..462,aa:Asp,seq:gtc)
     gene            549..809
                     /locus_tag="VN778_00450"
     CDS             549..809
                     /locus_tag="VN778_00450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49485.1"
                     /translation="MEDLQNIRSQLAEDMHDVAIEETIALETEQLPKLSLEIRARETM
                     RLNKEAVAHSEAFLVRTRSFREASQERVIRITRRLRAAGIDI"
     gene            1089..1598
                     /locus_tag="VN778_00455"
     CDS             1089..1598
                     /locus_tag="VN778_00455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49486.1"
                     /translation="MHQNQAVQEYSFGKITYKVGVDGNIDRPYAVIDHMLATATAVSS
                     VEAIQHGISDSQRDEGKQQQTCKASVEFGEISVTTEISFDLKHHVSRRTLNSLVFVAI
                     LPKVWQTAGIYLGDNQPVTTPQSPLAPMPRKDALDGIGSALGLEPLAGFDLSQQQNDQ
                     RSRQLVGAR"
     gene            1999..2478
                     /locus_tag="VN778_00460"
     CDS             1999..2478
                     /locus_tag="VN778_00460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49487.1"
                     /translation="MSHLSNFGRTNLRLTIVAGLAFFAILVLATVANAEGGVTRGNVE
                     MQYGYNGVLHRIVNTTTVYTGHIWKKSSQDGRLVTTKSGIYLKLVKGEKVVQAGIYYG
                     TISTPTYKDFPWSGRGTVPYFKQLKAKDGKIYKIKLSSVFVLVHEAGRPLPSLTGGK"
     gene            complement(2616..3140)
                     /locus_tag="VN778_00465"
     CDS             complement(2616..3140)
                     /locus_tag="VN778_00465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49488.1"
                     /translation="MAYEAKLRRQAFGLLSMLALQFLLGMILNLFVQLPTTHPGTSGA
                     YISRSLHGFGWAITIGGGIVLFLHVIVAIGLLLASLSLVVRAAATKSGSWLLTSIIGA
                     LGVLAALTNGLAFLGYNSDVNSFVMAVGFMVAAVSYTTALSFAMSPAQASKAKLEEAK
                     HTGHFFGRFRTSHG"
     gene            3245..>3828
                     /locus_tag="VN778_00470"
     CDS             3245..>3828
                     /locus_tag="VN778_00470"
                     /inference="COORDINATES: protein motif:HMM:NF013156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cohesin domain-containing protein"
                     /protein_id="HXR49489.1"
                     /translation="MRIKQKLFSAILGAVLLAPAFVQLALAATGVIYITPASSSLQVG
                     SSETLQLRINPGTTVDGVQATVSYNTGYLQLNSVDTSSSPFTVTLQKSAGGGTITLAL
                     GNLSGGVSSDSLIATMNFTALAGSSSSSLTISGANATSSGSYTNPATSGASVSFTSPV
                     RTQAPTSSGGGSSSSHASSGSGAQVSTPTATSSAS"
BASE COUNT          973 a         1066 c          972 g          817 t
ORIGIN      
        1 gccggcgccg aagccagggc cgaatcggcg cttaagcaac tgcaacacgg caccgatttc
       61 ggcacgctcg ccacgcaggt ctctgaagat cccgccacta aaaccaacgg cggccaatac
      121 ggcgcaccca tcacccaaag cgaccgcgac atcgcgccgg ccgtcaccac cgaactcttc
      181 aagctcaaac cgggccagat ctccggcatc atcaacacgg gatacacact ggagattgtc
      241 aaagtcatcg atcgcagcag tgacagcctc cacgctgccc acatccagtt caatctgcaa
      301 gacatcacga cctacaccaa gcccctggcc gccaaagagc cggcgcaccg ctacatcaag
      361 gtctagccac gaacaagttg ctttttctcc tgtaaattgc tagaattacc cctgtctttc
      421 cacttgggct cgtagtgaag aggcctatca cgcctccctg tcacggagga gatcgccggt
      481 tcgaatccgg tcgggcccgc caagcataaa cactttacat aacgtatttt ttatagcata
      541 ttgatagaat ggaagatctt caaaatatac gctcccagct agcagaagat atgcatgatg
      601 tagctattga agaaacaata gcattggaaa cagagcaatt gcctaaactc tcgttagaaa
      661 tcagggcgag agaaactatg cgcctcaata aggaagcagt tgctcactcg gaggcattcc
      721 tagtaagaac taggtcattc agagaagcga gccaggaaag agttatcaga attactcgta
      781 ggcttagggc tgcgggaatt gatatatagt tcggaaatcc gacccagtat cccgccaagc
      841 ctcgcatgta aaagatctta tagataaatt cggtggtatt tataatactg gtttttgtgc
      901 aaagccagcg tggcctttct ttagtttggc ccgggcctac agcactttga caagcaagcg
      961 atgagtatat acaagatgag cgataaacct ataaagcctg tagttttgtc gctcatcttg
     1021 tgtgttaaga atacgcacat tcgcgttagc gaacggcaca acagccaaag ttgagcagaa
     1081 agagtgtcat gcaccaaaac caggccgtgc aggagtactc cttcggcaag atcacgtaca
     1141 aggtcggcgt tgatggcaac atcgaccgcc cctacgcggt gatcgatcac atgctggcga
     1201 cggcgacggc agtttcaagc gtcgaagcca tccagcatgg catatctgac agccagcggg
     1261 acgagggcaa gcagcagcag acgtgcaagg cgtctgtcga gttcggcgaa atctcggtca
     1321 ccactgagat cagcttcgac ctcaagcacc acgtttcgcg acgaacgctc aacagcctgg
     1381 tcttcgtcgc catcctaccg aaggtctggc agaccgccgg catctatctg ggggacaacc
     1441 agccggtgac cacgccgcag tcgcctctgg cgccaatgcc aaggaaggac gccctggacg
     1501 gcatcggcag cgctttgggg ttggagccgc tcgccggttt cgacttgagc cagcaacaga
     1561 acgatcagcg ttcacgccag ctcgtcggcg cgcgctaacc agctcgcact actgaaggta
     1621 acgacaggcg gcgagctgaa tactacacgc tcctcgcgtg tatcttttcc tctcgcttgc
     1681 ttgtcgttac cctagctagt tacttatact ggagttagat gccaactgat aacgaaaaag
     1741 ccaagtgcaa acgaaaaacc cgctatggaa gcgactatga agcccagcaa tcgatcaaaa
     1801 gaatccggct caagcgtggg gcgggtaaat cgttacgcac ttatcgttgt cctgtttgtt
     1861 taggctatca tataacttca agcacaaggc ggtagctccg agaccagcca catattgtat
     1921 tttgataacg tttatgctat aatatgacct agtggagcaa ttgcttcatg tccaaacgag
     1981 ttcgggaggc atccctacat gtcgcacctt agcaattttg gtcggaccaa cctgcggctc
     2041 accatcgtgg ccggtctcgc attctttgcg attctcgtgc ttgcgacggt cgctaacgct
     2101 gaaggtggtg tcacccgtgg caatgtcgaa atgcagtacg gctacaacgg cgtgcttcac
     2161 cgcatcgtca acacgaccac ggtctacacc gggcacatct ggaagaaatc ctctcaggac
     2221 ggccgcttgg tcaccaccaa gtcgggcatc tacctcaagc tcgtcaaggg cgagaaggtg
     2281 gtgcaggccg gtatctacta cgggaccatc agcacgccca cctacaagga tttcccttgg
     2341 agtggacggg gcacggtgcc gtacttcaag cagttgaagg ccaaggacgg caagatctac
     2401 aagatcaagc tctcgagcgt tttcgtgctc gtgcacgaag ctggccggcc gcttccgtca
     2461 ctcacggggg gcaaataagt cgaagcagcc agggaggcgg gattgaactt cacgttcttc
     2521 cgcctcccgg cgctctcttt acccccttat gctatctcga actcgttttt accattatga
     2581 aatgtcgaaa gtatagcctc gaaacagttg cttgattagc cgtggctagt gcggaatcgg
     2641 ccgaagaagt gcccagtatg ttttgcttcc tctaactttg ctttagatgc ctgtgcgggg
     2701 ctcatagcga aagataaggc ggtagtgtag gagacggccg cgaccataaa gcccaccgcc
     2761 atcacgaacg aatttacatc actgttataa cccaaaaatg ccaagccgtt cgttagagcg
     2821 gccagtacgc caagtgcacc gataatgcta gtcaacagcc acgagccgct cttggtcgct
     2881 gccgctctca ctaccaagct cagtgatgcc agcagcaggc cgatggcgac gataacgtgc
     2941 agaaataaca cgataccgcc gccaatggta attgcccaac cgaaaccatg taaactgcgt
     3001 gagatgtatg cgccgctagt accgggatga gttgtcggca gctgcacaaa taaattaagg
     3061 atcatgccca agagaaattg gagggccagc atggataaca gcccaaaagc ctgtctccgt
     3121 agtttcgctt catatgccat gcatcaaaat atatattcag gtgaggcagg ggtctatgag
     3181 taatgttaca ttgtcataga gctagcttat gcttataata agaatgcact cctaaaaaca
     3241 aaaaatgcgt atcaaacaaa aacttttcag tgcgatactt ggcgccgtcc tcctggcgcc
     3301 agcttttgtg caattagcgc tggcggccac cggtgtcata tatataacgc cagcttcgag
     3361 tagcttgcag gtcggcagct cggaaacgct gcaactcagg attaaccccg gtacaaccgt
     3421 ggacggggtg caggccaccg ttagctacaa caccgggtat ctgcaattga atagcgtcga
     3481 tacctcgagc tcaccattta ccgtcacgct gcagaaatca gcgggtggcg gcacgatcac
     3541 cctggcgctt ggtaacctct caggtggggt gagcagtgac tcgttgatcg caaccatgaa
     3601 ttttacggcc ctggccggca gcagcagttc ttcgctgacc attagtggcg ccaatgccac
     3661 tagttccggg tcttacacga acccggcaac ctccggcgcc tctgtcagct tcacttcacc
     3721 ggtccgtacg caagctccga catccagtgg tggcgggtcg tcatcatcgc atgcttcatc
     3781 ggggagtggt gctcaagtca gtacacctac ggccacaagc agcgcgtc
//
LOCUS       DATNGP010000019         7379 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_2092084, whole genome shotgun sequence.
ACCESSION   DATNGP010000019 DATNGP010000000
VERSION     DATNGP010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 7379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7379
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2092084"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..515)
                     /locus_tag="VN778_00475"
     CDS             complement(<1..515)
                     /locus_tag="VN778_00475"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011231937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HXR49490.1"
                     /translation="MFKVPDLDYGYDAFGTYISHAIMELHHDKHHQTYVDKLNAALEQ
                     APELQSKSLEELVRSVASLPEQVRTTIRNNGGGHYNHSLFWKIMSPDGGGEPTGQLAD
                     ELKEKYGSFQGFMDKFGEAATGIFGSGWAWLQPDLSIVTTPNQDSPLNSGGPDPILGL
                     DVWEHAYYLDY"
     gene            660..2150
                     /locus_tag="VN778_00480"
     CDS             660..2150
                     /locus_tag="VN778_00480"
                     /inference="COORDINATES: protein motif:HMM:NF012690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HXR49491.1"
                     /translation="MPVIPNKAQFFQSLNEQGLAVTYDDVRLRTIVKPAQRVTAVETN
                     LVSRFSRNVELKIPIVSSPMDTVTGSEMAIAMAKVGGLGIIHSANSIDEQLAEVQRVK
                     FHRNGLIEKPVTVSLTDTVAAVVERRRLAQYDFSTFPVIDKQGRLVGIFTQSDVDYCR
                     NPSEPVKKHMRPLKQLVYATGKTSPKAAYDIMYREGKLNTLPLIDKNGLLIGLYIKSD
                     VLRVIQDNPKNYNLDTNGRLRVGAAVPTDPEEASERLKAIGEFIDVVVIDTANGNSRY
                     LFETLARIKKETTLDVVAGNVSEGYSARLLAKAGADGIRVGQGGGSICTTRRETGIGT
                     PQVTAVYECAQAVEEFGIPVCADGGIKDKGDIAIALAVGAENVMLGNMLAATKESPGE
                     MTVLENGTIVMEYRGMGSPSALRDSIASRKRYGGSSTGTPLPEGVEAQLLYKGSVFDV
                     VPVYVKALRKALEYVGAPDLEHLRRETLLWRITNAGLRESNPHDLL"
     gene            2185..3099
                     /locus_tag="VN778_00485"
     CDS             2185..3099
                     /locus_tag="VN778_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013425153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXR49492.1"
                     /translation="MDIAVKTQGLGKKYGSFEALAPLDLEVKKGEVLGYLGPNGAGKT
                     TTIRLLLGLIKPTSGHAEIFGINAQTQKVEAHKRIAYVPGEATFWPSLTGAETLHLLS
                     TVHGKTDEKYQSELIERFQFDPSKKVRTYSKGNRQKINLIAALSTRAELLIMDEPTSG
                     LDPIMEQAFRQSVLEAKINGQTVFLSSHILEEVEALCDRLAILRAGHLVELGTLSEMR
                     HLSALHVEATFKDKPPKIEHLKGVSGVKIVGSHLTCQVQGPIDPLLSLIASHHPKTLL
                     SREPSLEELFLALYGDDEVRNETGSNEH"
     gene            3074..4717
                     /locus_tag="VN778_00490"
     CDS             3074..4717
                     /locus_tag="VN778_00490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49493.1"
                     /translation="MKRVAMSTKMYRSPRTVIARFVARRTMRSATIGGLIFGVYVASK
                     AIGLVKAYPTAASRATLAHTFSNNIGLNALLGVPHRIDTVPGYVAWNGLMVLTIVGSI
                     RGFLFATKYFRGEEDAGRSEILLTGQTTQKRAAVNILAGFGISLLYFYAIVALMFVAV
                     GSRHDIGLTAQSSLFFALACVSGVALFLAVGALASQLMPTQGRASSVAAVVFGVSFLL
                     RAMADSTSMHWLLNITPIGWIERLQPLAGSQPIWLLPIFGTVAILCGLAIWLAGRRDL
                     GASIIADKDSSEPRTALLNSPFGLSLRISRNSIIGWLVGVGFMGAFYGLLTNAAVQAF
                     TQAGAARKAFKRLEHSSQASISTLFLGFVFFILLPMIMAYVASAISHVRAEEAEGYVD
                     NFLVRPISRLRWLGGRVLLIAAVGVIACVVGSVGTWAGQVSQHTGVTFHALFLAGLNM
                     VAPVFFTLGASVLAMGFVPRLTNLAAYGIIGWSFLLSMISSGVNLSHWILDTSLLHQI
                     VLAPAANPNWKTNAIIGTLGLALATAGAWRFNSRDLQTE"
     gene            complement(4726..5532)
                     /locus_tag="VN778_00495"
     CDS             complement(4726..5532)
                     /locus_tag="VN778_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011693722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49494.1"
                     /translation="MKKMTLKPPSPRLLLTVISLIAILSPTLGVAAQGAPRWNTKTAA
                     VQTGNDVSWPQCGKTLPKGQLFSIVGVNDGLANNTNPCLSTELSWANTSTGTTTQPKA
                     ALYVSTANPGNLGVADWPTSGTNAYGTCAGGDDRACAYQYGWNMAQADALNRGVPNGT
                     NYKWWLDVETVNSWEINTANNVADLEGMTAYLESVGSTVGVYSTGYQWDKIAGAVNSS
                     SNLYALTDWLPGARSLSGAQSNCRLTPLTAGGQVVVTQYVSSKIDYDYAC"
     gene            complement(5504..5929)
                     /locus_tag="VN778_00500"
     CDS             complement(5504..5929)
                     /locus_tag="VN778_00500"
                     /inference="COORDINATES: protein motif:HMM:NF015177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49495.1"
                     /translation="MSFNTPYPLPSIATDSRNYDPSNREKDRLWRGGVWMNTNKLVEH
                     GLRKQLARFQSDLAHRPDLMKRSQDWAERIDKRSRFLVKSKGFWEHYNPQNADPLRTR
                     VKKFAWSNLALIYIVNAADRFLQQKQLQYEYEENDAQAA"
     gene            complement(5957..6988)
                     /locus_tag="VN778_00505"
     CDS             complement(5957..6988)
                     /locus_tag="VN778_00505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49496.1"
                     /translation="MERLHLSEGDAGPLDSEIAAQNIFERQTEHGPDTRYTRPSPHYE
                     GLYLWDSFFAAWINAVAGRQAAEQGLPTAPRWLTAAQAELMAITDGQNENGFIPNIQN
                     FERKIDFERIIAFGKGSQQSNYTQPPVFALPILETHEAALRTAELSGDTSLAEESRAW
                     LQTIYPKASAFYKYFENVLSNSPQDKLIGLYHPHMSGRDSDPTFDRGLKRWRLKYNGL
                     ATKEWRYLANIAIDYASILKHGWDLRKAGGDVQKMREMYWMNDVMMNCIYADNLFYMA
                     EIASQTSHDQDAEAFTALALDVERQILTRMWNEKDKRFYALDSEGKYLKEMTVSNLFP
                     LLLPNLQEE"
     gene            complement(7044..>7379)
                     /locus_tag="VN778_00510"
     CDS             complement(7044..>7379)
                     /locus_tag="VN778_00510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49497.1"
                     /translation="ELVVPEHQAAIQEYLQISGANPARALHDTKHIEEAARQGRVDKL
                     LTRMTTQTTDNVQDSVVDMPRLTFPASSESKHLNDVALHVWRSSGTIYNLLPGEMPNG
                     ALMAASLRY"
BASE COUNT         1690 a         1853 c         1972 g         1864 t
ORIGIN      
        1 aagtagtcca aatagtaagc gtgctcccaa acatcaagac cgaggatcgg atcaggtccg
       61 ccggagttca acggcgaatc ttggttcggg gtcgtcacaa ttgataagtc aggttgcagc
      121 cacgcccagc cactaccgaa aatgcccgtg gcggcttcgc cgaacttgtc cataaagcct
      181 tgaaagctac cgtacttttc ttttagctcg tctgccaact gacccgttgg ttcgccgccg
      241 ccgtcaggcg acatgatttt ccagaaaaga ctatgattat agtggccgcc accgttgttg
      301 cgaatggtgg tgcgcacctg ctccggtaag ctagcaacac tgcgtaccag ttcttctagt
      361 gatttacttt gcaattcagg ggcttgttct agagcggcgt ttaatttgtc gacataggtt
      421 tgatgatgtt tgtcgtgatg cagctccata atggcgtggc tgatgtacgt gccaaaagcg
      481 tcgtagccat agtccagatc aggtacttta aacatgtgcg ggaacctcct tatatattct
      541 aaaattatag gtgatttccg agtaaaatgc gtataaaaaa atttactccg ggccctgagg
      601 aggcttaggc gggcggagta taaggtggta caatttgtaa aacatagtag gtggctcaca
      661 tgccggttat cccaaataaa gcacaatttt ttcagtctct caatgagcag ggtttggcag
      721 tgacttatga tgatgtgcgg ctgcgcacca ttgtcaaacc ggcgcaacgc gtgacggctg
      781 ttgagaccaa ccttgtcagt cgattttctc gcaatgtgga actaaaaatt ccaatcgtta
      841 gctctcccat ggacactgtt acgggctcgg agatggccat cgccatggct aaagtcggcg
      901 ggctcggcat tattcattcc gctaatagta ttgatgagca gttagcagaa gtacaacgtg
      961 tcaaatttca tcgaaatggt cttatcgaaa agccagtaac ggtgagtcta acagataccg
     1021 tggccgcagt ggtagagcgc cgccgcctgg cccagtatga cttctcaacc tttccagtta
     1081 ttgataagca agggcgctta gtaggcatat ttacccagtc cgacgtcgat tattgccgga
     1141 acccttcaga accagtcaaa aaacacatgc ggccgttgaa acaattggtg tacgccaccg
     1201 gcaaaacttc cccgaaagcc gcctacgaca ttatgtatcg ggaaggtaag cttaacacgc
     1261 tgccccttat tgataagaac ggtctgctca ttggcctgta tattaagtct gacgtgttac
     1321 gggtgattca agacaacccc aagaactata atcttgacac caacggccga ctgcgtgttg
     1381 gcgcggccgt gcccactgat cccgaggagg cctccgagcg cttaaaagct atcggcgagt
     1441 ttatcgacgt ggtggttatt gatacagcta atggcaattc gagatatttg tttgagaccc
     1501 tggcccgcat taaaaaggag acgacactcg atgtggtagc cggcaatgtt tccgagggct
     1561 actctgcacg tttactggca aaagccggcg ctgacggcat ccgggttggc cagggcggtg
     1621 ggtccatctg taccacgcgc cgggagaccg gtatcggcac gccgcaagtc accgccgtct
     1681 acgagtgcgc ccaagccgtc gaagagtttg gcattcccgt ttgcgccgat ggtggcataa
     1741 aagacaaggg cgatatcgca attgctttgg cagtcggcgc cgagaacgtt atgctcggca
     1801 acatgctggc cgcaaccaaa gaatcgcctg gtgaaatgac cgtgctggaa aacggcacca
     1861 tagttatgga gtaccgtggg atgggttcgc catcggcttt gcgtgatagc attgccagtc
     1921 gcaagcgtta tggtggtagc tccaccggca ctcccctgcc cgaaggcgtg gaagcgcagt
     1981 tgctgtataa agggtcggtc tttgacgtag tcccggtcta tgtcaaagcg ctgcgtaagg
     2041 ccctggaata cgtcggtgca cccgatctag aacatttgcg ccgcgaaacc ctactctggc
     2101 gtatcacgaa tgccggtctc cgtgaatcaa atccccatga ccttttgtag tataaaataa
     2161 ccggcgcaca tttggaggaa aaacatggat attgcggtaa aaacacaggg attaggcaaa
     2221 aaatacggct cattcgaagc gctcgcacca cttgatttgg aggtcaaaaa aggcgaagtc
     2281 cttggctatt taggcccgaa tggcgcgggc aaaacgacca ccattcgcct cctgcttgga
     2341 ttgataaaac cgacatcggg ccatgccgaa atttttggca tcaatgctca aacacagaaa
     2401 gtagaggccc acaaacggat cgcttatgtc ccaggcgaag caaccttctg gccgtcactg
     2461 actggtgcgg aaacgctgca tttgctgagc acggttcacg gcaaaaccga cgaaaaatat
     2521 cagtctgagc taatcgagcg cttccagttt gatccgtcga aaaaagtacg aacgtattcc
     2581 aaaggtaacc gccaaaagat aaaccttatc gccgctcttt cgactcgcgc cgagctgctc
     2641 attatggacg aaccgaccag tggtttggat ccaatcatgg agcaggcctt tcgccaaagc
     2701 gtcctcgaag ccaaaataaa tggccagacg gtatttttat catcgcatat tctcgaagaa
     2761 gtggaagcct tatgtgaccg attggcaatt ttgcgtgccg gtcatttggt agagctcggc
     2821 acgctgagtg aaatgcgcca cctatcggct ttacatgtcg aagcgacatt caaagataaa
     2881 ccgcccaaaa ttgaacatct aaaaggcgtc agtggagtca aaattgttgg atcacactta
     2941 acctgtcaag ttcaggggcc tatcgatccc ttgctgagcc tcatagcttc tcatcatccc
     3001 aagactttac tcagtcgtga gccttcgctc gaagagctgt ttttggcgct gtatggcgac
     3061 gatgaagtgc gtaatgaaac gggtagcaat gagcactaaa atgtaccgat cgccccgaac
     3121 ggtcattgct cgattcgtgg cccggcgaac catgcgcagc gctacaatcg gagggctgat
     3181 ttttggcgtt tatgtagctt ccaaggccat tggcctagta aaagcctatc caacagccgc
     3241 ctcgcgggcg acgctcgcgc atacctttag taataacatt ggcctcaacg ctttgctcgg
     3301 cgtgccgcac cgcattgata cagtgcctgg ctacgtagct tggaatggac tcatggtatt
     3361 aacgatcgtc ggctcgattc gtggcttttt gtttgctacc aaatattttc gcggtgaaga
     3421 agatgccggt cgcagtgaaa ttttgctgac cggtcaaacg acacagaagc gagcggcggt
     3481 caacattcta gctggatttg gcatcagcct gttatatttt tatgcaattg tggctcttat
     3541 gttcgtggcc gttggtagcc ggcatgacat tggcctgacg gcgcagagct cattattttt
     3601 tgcgttggcg tgtgtgtcgg gggttgctct tttcttggct gtcggcgctc tagccagcca
     3661 gctgatgccg acgcaggggc gggcttccag cgtggcagct gtcgtgttcg gggttagttt
     3721 cttgctccgt gctatggctg attctacgag tatgcactgg ctgcttaata tcacgccgat
     3781 tggctggatc gagcgcttgc agccgctggc cggcagccag ccaatttggc ttttgccaat
     3841 tttcggcacc gtggctattc tctgcggact ggctatttgg ttggctggcc gacgtgatct
     3901 gggggctagc attattgcag ataaagacag ttctgagcca cgcactgcat tattaaattc
     3961 accgtttggc ttgagtttac gcatatcacg aaactcaata attggctggt tggtaggcgt
     4021 tggtttcatg ggcgccttct atggcctgtt aactaatgcc gccgtccaag cctttacgca
     4081 agccggtgcg gcccgcaaag cctttaagcg cctagaacac tcctcccaag cttcgatcag
     4141 cacgctgttt ctcgggtttg tcttttttat cctgctgcct atgatcatgg cgtatgtagc
     4201 tagtgccatc agccacgtgc gcgcggagga agctgaaggc tacgtcgata attttctggt
     4261 gcgcccaatt agccggttac gctggctcgg gggtagagtg ttattgattg ctgctgttgg
     4321 tgtcatcgcg tgtgtggtgg gcagtgtggg cacatgggcg ggccaagtca gccaacacac
     4381 cggggtaacg ttccatgccc tgtttctcgc tggtcttaat atggtagcac cggtcttctt
     4441 cacccttggc gctagtgttt tggcgatggg ttttgtcccg cggctgacga accttgcggc
     4501 ctacggcatt attggctggt ctttcctgct ctccatgatt agtagtggtg ttaatctgag
     4561 tcactggatc ctcgacactt ccctgctgca tcagatcgtt ttagcgccgg ctgccaatcc
     4621 caactggaaa accaacgcta ttattggcac ccttggccta gctttggcta cagccggtgc
     4681 ctggcgtttc aacagccgcg atttgcagac cgaataatcg actagttagc aggcgtaatc
     4741 gtagtcaatc ttggaagaca catattgcgt aacaacgact tgcccgccag ctgtgagtgg
     4801 cgtcagccgg caattagact gcgccccaga tagcgaacgg gcaccgggca gccagtctgt
     4861 tagagcgtac aagttgcttg aggaattgac cgctcctgcg atcttgtccc attgatagcc
     4921 ggtagaataa acgccaactg tactaccaac gctttcaaga tacgcagtca ttccttcaag
     4981 gtcggctaca ttattggccg tgtttatctc ccaggaattg acggtttcga catcaagcca
     5041 ccacttgtag ttagtaccat ttggcacacc ccgattcaga gcgtcagcct gggccatatt
     5101 ccagccatac tgataggcac aggctcgatc gtcaccaccg gcgcaggttc cataggcatt
     5161 ggtgccggag gtgggccagt cggctacgcc aaggttaccg gggttagcgg tactgacata
     5221 tagagcagct ttcggctgtg tcgtcgtacc ggtggaagtg ttggcccagg ataactcagt
     5281 cgataggcac gggttggtat tgtttgccag accatcattg acgccaacta tgctaaagag
     5341 ctggcccttg ggtagcgttt tgccgcattg cggccaagaa acatcattac ctgtctggac
     5401 ggcagccgtt ttggtgttcc agcgtggtgc cccttgggca gctacaccga gtgttgggct
     5461 gagaatagcg ataagactta taacagtaag caacaatcgt ggactaggcg gcttgagcgt
     5521 cattttcttc atattcatat tgtaattgtt tttgctgaag aaagcgatca gcggcattca
     5581 ctatatagat aagagccaga ttggaccagg caaacttttt aacgcgggtg cgcaacgggt
     5641 cggcgttttg gggattgtaa tgctcccaaa accccttgct tttcactaag aaacgtgacc
     5701 gcttatcaat acgttcggcc cagtcctggg accgtttcat cagatctggg cgatgtgcta
     5761 agtcggactg aaaacgagcc agttgtttgc gtaagccatg ctcgacgagt ttattagtat
     5821 tcatccacac accgccgcgc cacaaacggt ctttttcgcg gttgctagga tcatagttac
     5881 ggctatccgt agctatactc ggcaatggat agggggtgtt aaaagacaca tccagagtgt
     5941 ccaggagcgc ttggagctac tcttcctgca gattgggcaa taataacgga aacagattgc
     6001 tcacagtcat ctctttaaga tacttgcctt cgctatccaa ggcgtagaat cttttatctt
     6061 tttcgttcca cattctcgtc aggatttgcc gttcgacgtc caaggcgagc gcggtgaaag
     6121 cctccgcgtc ctgatcgtgg ctggtttgac tggcaatttc ggccatataa aagagattgt
     6181 cggcatagat gcagttcatc atgacgtcat tcatccagta catctcacgc attttttgaa
     6241 cgtcgccgcc ggctttacgc aaatcccagc cgtgtttcaa aatagaagca taatcgatgg
     6301 cgatattggc cagatagcgc cactctttgg ttgccaggcc attgtactta aggcgccaac
     6361 gcttcaagcc acgatcaaac gtcgggtccg agtcgcgccc tgacatgtgc ggatggtaca
     6421 aaccgattaa tttgtcctga ggactattgc tcaggacatt ttcgaagtac ttataaaaag
     6481 cgctggcttt cggatagatc gtttgcagcc aggcgcgtga ttcttcggca agtgaagtat
     6541 cgccgctgag ttcggcggtc ctgagagcgg cttcgtgggt ttcgaggatg ggtaaggcaa
     6601 agaccggcgg ttgggtgtaa ttgctctgct ggcttccctt gccaaaagcg ataatgcgct
     6661 caaaatcaat tttacgctca aaattctgga tattaggtat gaaaccgttc tcgttctgtc
     6721 cgtcagtgat tgccatgagc tccgcttgcg ctgctgttag ccagcgcgga gcagtgggta
     6781 gcccctgctc ggcggcttgt ctgccggcaa cagcgttaat ccaggctgca aagaaggagt
     6841 cccacaaata caaaccttca taatgcggcg aaggccgggt gtagcgggtg tcagggccgt
     6901 gttccgtttg tcgttcgaag atgttttgag cagctatttc tgaatcaagc ggaccggcat
     6961 cgccttcgga taggtgcaag cgctccataa ttaggccagt ataacagacg ggctagtatc
     7021 tttggctaga cgggtgctcg atgtcagtag cgcaagctgg cagccatgag cgcgccattc
     7081 ggcatctcac ccggcaacaa attgtagatg gtgccgctac tgcgccagac atgcaacgcg
     7141 acatcgttga ggtgtttgct ttcggaggac gcggggaaag tcaggcgcgg catatccacg
     7201 acactatcct gcacattatc agtagtttgg gtagtcatgc gggtgagtag tttatcgacc
     7261 ctgccctgcc gtgccgcttc ttcgatatgc ttagtatcgt gcaaagctcg agccggattg
     7321 gcgccgctaa tctgcaagta ttcctggata gcggcttggt gctccggtac aaccagctc
//
LOCUS       DATNGP010000020         3124 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_1350393, whole genome shotgun sequence.
ACCESSION   DATNGP010000020 DATNGP010000000
VERSION     DATNGP010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3124
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1350393"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..508)
                     /locus_tag="VN778_00515"
     CDS             complement(<1..508)
                     /locus_tag="VN778_00515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49498.1"
                     /translation="MCSSPKRAWRKLIIGSALLGVVSVCLIFTALPVRAASGINQQLN
                     FQGRLLNSSGATVADGVYNIEFKIYQDGDGQSVGDATGSPAGTLKWTEDHLVSASTGA
                     TIRNGYLSVQLGSVTAFGSSIDWNQDTLWLSVNIGNTSSCTITTSFNANCGGDGEMVP
                     MKRLSSSPY"
     gene            complement(661..1644)
                     /locus_tag="VN778_00520"
     CDS             complement(661..1644)
                     /locus_tag="VN778_00520"
                     /inference="COORDINATES: protein motif:HMM:NF012999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HXR49499.1"
                     /translation="MPIKTKIIHVKHTGRLRPHEHTSYVSLAIFVLITGGVLTLFSVA
                     GLASASPGPASGSVSLSGTLPARAPTTAAVIGNPANGQHFTTSPVDVSGSCPVQTLVV
                     IYKNDIFAGSTPCSNAGAFSLKVDLLIGKNILIARVFDALNQAGPDSSPVTTFYDILP
                     PQAGPLAVLNLPEKQLLLITQPFYRGVFPGQSLNVPITILGGVAPYAINVEWGDSTNK
                     ITPRGDSSTFNAAHIYQKPGTYTVTIQASDSQGRIAFLQITAIVNGQPDVIAASNTSD
                     RTTTTNRLLVLWPLYAIAAAVVVSFWLGEQREKRIIAAQEARMQPILTPVS"
     gene            complement(1681..2676)
                     /locus_tag="VN778_00525"
     CDS             complement(1681..2676)
                     /locus_tag="VN778_00525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49500.1"
                     /translation="MTKRFYLTLIIATIALPYVAMAPVVLAATTPNPQNAGQALEIAP
                     PVITLTLDPGQTATTQIYLRDIASTKLNVTGQANDFVAAGEDGTPKVLLDTTGSGPYS
                     LRNWVGALPTLQLIPREIKSMAVTITVPRDASPGGHYGVIRFTAVPPDLKDTGVSLSA
                     SLGALLLVTVRGKLIDNLELQSFTVNQDGKSSTFFEAAPLTFSQLLRNSGNVHEEPAG
                     QVTITDMFGAKLAVLGINQPPHNVLPASSRRFDEVLDSTVIGNKMLFGRYTATLKATY
                     ANGKSVNGSLTFWVVPYRLIAVIVVAGVGGFFGLRYGIRRYNRFIVGQSRRGRRY"
     gene            complement(2683..>3124)
                     /locus_tag="VN778_00530"
     CDS             complement(2683..>3124)
                     /locus_tag="VN778_00530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49501.1"
                     /translation="TATSQMAASTNAGSGYSITVNGPTLTSGSNTITAMSSAAVGVRG
                     TSQFGLNLRANTTTTSTPAVGIDVTPAAGAGGGTFKGEAATGYSTPDQFKFVSGNSVA
                     DSSFGGAGPTDAQIFTVSYIVNVAGSQAAGTYTSTLTYICTPTF"
BASE COUNT          723 a          770 c          871 g          760 t
ORIGIN      
        1 catacgggct ggatgatagg cgcttcatcg gcaccatttc gccgtcgccg ccacagttcg
       61 cattgaagct tgtagtgatg gtgcaggatg aagtgttgcc gatattgacc gacagccaga
      121 gcgtatcctg gttccagtca atgctgcttc caaaggccgt tactgaaccg agctgtaccg
      181 acagataacc gttgcggata gtagcacccg tactagccga aactaaatgg tcctcggtcc
      241 atttcagcgt cccagccgga gatcctgtag cgtcgccgac agattggccg tcgccgtcct
      301 gatagatttt aaattcaatg ttatacacac cgtctgcgac ggtggcgcca ctgctgttca
      361 gtagacgtcc ctggaagttg agctgctgat taattccact ggctgcccga accgggagag
      421 ccgtaaaaat tagacaaaca ctaacaaccc ctagaagggc gctaccaatt atcagctttc
      481 gccaagctct ttttgggctt gaacacatgt ttatgtttgt ttggtttggt taattttggt
      541 ttacagcgta gctgatcctg agagtacaat aacccgcaag tataaacact acacctactt
      601 aagcataacc agtctcaatc tgtttgccaa ggctttttga gcctaattag tgttcaaaaa
      661 ttagctaaca ggggtcaaaa taggttgcat tctggcttcc tgtgccgcga tgatccgttt
      721 ttcgcgttgt tcaccgagcc aaaaacttac gactactgcc gctgcaatgg catatagtgg
      781 ccaaaggaca agcaaacgat tggttgttgt cgttcgatca gaggtgttgc ttgcggcgat
      841 gacatcgggc tggccattca caatagcggt gatctgtaga aacgcaatac gtccctggct
      901 gtcgctcgct tggatggtaa cggtatacgt gcctggcttt tggtagatgt gcgcggcgtt
      961 aaaggtactg ctgtcgcccc ttggcgtaat cttgttggtt gagtcgcccc actcaacgtt
     1021 gatagcatat ggcgctacgc cgccgaggat agtaatgggc acgttgagcg actggccggg
     1081 aaagacgcca cggtaaaagg gttgggtgat tagaagcagc tgcttttctg gcagattcag
     1141 taccgccaac gggcctgctt gcggcgggag tatgtcgtag aaggtggtta caggcgaaga
     1201 atctggtccc gcctggttga gcgcgtcaaa gacccgcgcg atcaggatgt ttttgccaat
     1261 caacagatca accttgagcg agaatgcgcc agcattgctg cacggtgtag agccagcgaa
     1321 gatatcgttc ttgtaaataa ccaccagtgt ttgcaccgga cagctgcccg acacgtcaac
     1381 gggcgaagtc gtaaaatgtt ggccatttgc cggattgcct ataactgcgg cagtagttgg
     1441 tgcgcgggca ggcagcgtgc cgctgaggct gaccgaaccc gaagctggcc ctggactggc
     1501 cgaagccagg cctgctaccg agaatagggt caagacccct ccggttatga gtacgaaaat
     1561 cgcaagcgat acatacgatg tatgctcgtg gggcctaagc cggcctgtgt gtttaacgtg
     1621 aatgattttt gtttttatag gcacaagcac agtataacat ttgtgtctag acaataggga
     1681 ctagtagcgg cggccgcgtc gcgactgccc aacgataaag cggttatagc ggcgaatgcc
     1741 gtagcgcagt ccgaagaaac caccgacgcc agcgaccacg atgactgcaa tcaagcggta
     1801 tggtaccacc cagaatgtta ggctgccgtt aactgactta ccgtttgcat aggtagcttt
     1861 tagcgtggcg gtgtagcggc caaacagcat cttattacca atgactgtgc tgtcgagtac
     1921 ttcatcgaag cgacgcgagc tcgctggcaa tacgttgtgc gggggttggt tgatgccaag
     1981 gactgccaac tttgccccga acatatcggt gatagtgacc tggccggctg gttcttcgtg
     2041 tacgttgcca gaattacgta gcagctggct aaacgttagc ggtgccgctt caaagaacgt
     2101 gctgcttttg ccatcctgat tcacggtgaa cgattgcagc tcaagattgt cgataagctt
     2161 tcctcgcacg gtcacaagca gtaaggcgcc aagactggct gacagtgaca cgccggtatc
     2221 cttgaggtct ggcgggacgg cagtaaaccg aataacaccg tagtgtccac caggggaggc
     2281 gtctcttggt acggtaatag tgaccgccat ggatttgatc tctcttggta tcagctgcaa
     2341 ggttggcagg gcgccaaccc agtttcttag cgagtacggc ccgctcccgg tagtatcaag
     2401 cagaactttt ggcgtcccgt cctctcccgc ggcaacaaaa tcgttggcct ggccggtaac
     2461 attaagcttg gtagacgcaa tgtcacgcaa atatatttga gtcgtcgctg tttgccctgg
     2521 gtcgagcgtc aaggtaataa cgggaggtgc aatttcaagg gcctgaccag cattctgtgg
     2581 gtttggcgtt gtggcagcaa gcacaacggg cgccatagct acatacggta gcgcaatagt
     2641 tgcgataata agtgtgaggt aaaatcgttt ggtcattatt ttttagaagg ttggcgtaca
     2701 gatatatgtc agagtagagg tgtatgtacc tgctgcctga ctacccgcta cgtttactat
     2761 atacgatacg gtgaatattt gcgcatctgt tgggccagct ccaccaaacg agctatcggc
     2821 tacactgttg ccgcttacaa acttgaactg atccggagtg ctataacccg ttgcggcctc
     2881 acccttgaat gtgccgccgc ccgcgcctgc ggctggtgtt acgtcaatgc caacagctgg
     2941 cgtggaagta gtcgtggtgt tggcgcgaag gttcaggccg aattggctag tgcctcgcac
     3001 acctacagcc gcagatgaca tggcagtgat ggtgttagaa cccgaagtaa gcgtcggccc
     3061 attaacggta atactgtatc ctgaaccagc attcgtgcta gcggccattt gtgaggttgc
     3121 ggta
//
LOCUS       DATNGP010000021         2176 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_703326, whole genome shotgun sequence.
ACCESSION   DATNGP010000021 DATNGP010000000
VERSION     DATNGP010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2176
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_703326"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..343
                     /locus_tag="VN778_00535"
     CDS             <1..343
                     /locus_tag="VN778_00535"
                     /inference="COORDINATES: protein motif:HMM:NF013212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HXR49502.1"
                     /translation="IANLRTHLKANIVRELLPVIDNFERALKHTPKELIDNDYIKGVQ
                     GIVRQFEKTLEQLGVQRIKTVGEPFDPRAHEAVSMEEGGSGQEIVSEELQSGYILGDD
                     VIRHAMVRVKV"
     gene            407..>2176
                     /locus_tag="VN778_00540"
     CDS             407..>2176
                     /locus_tag="VN778_00540"
                     /inference="COORDINATES: protein motif:HMM:NF014044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HXR49503.1"
                     /translation="MSTETTTQPSPDQDTVVLLLQVPRTNDKKELAAEQMFASLHGLL
                     TFPSQKMFQPDRRVRVSFEIAVINRRIGFYVCVPRYLVGFVEEQIYAQYPMVQISEVA
                     DYAQADEVPQGSLVTEMKLSANDALPIKTFQSFEVDPLAAITATLAKFTEDEQAWVQL
                     IVQPASKNWHKKSERYIGTLRGGRGGGSLAGGLFSALWAPPDNSGTAAPKLTEYDQAR
                     ASGAEDKSHKLAFETSLRIVYKGNVSPQEAELRLQSIIASYKQFNTTYLNGFQQTKLA
                     SDAALLERYRKRALGKRPLLLNIEEVATLYHLPHTSVETPYILWASSQTAEPPANLPI
                     VNETNMDVVSPIAVANFRGHNAAFGMPRGDRGRHLYILGQTGVGKSGLLELLTISEIS
                     NPYGFAVIDPHGDYALSVLKRIPASRAQDVIYFNPADTDFPIAFNPMEVSDPKLRTHT
                     VSELVGVLKRMFDSWGPRLEYILRYCLLALLEYPEATMLDITRILTDKSFRGQVLQYV
                     TDPVVRNFWTVEFATWNDRFAAEAVAPVLNKVGAFTANPLVRNIIGQPKSSFNIRQIM
                     DERKILIVNLSRGLVGEDNAALLG"
BASE COUNT          531 a          577 c          578 g          490 t
ORIGIN      
        1 aattgctaat ctgcgcacgc atctgaaggc taatatcgtc cgcgagctat tacctgtcat
       61 tgacaatttc gaacgtgccc tcaagcatac acccaaggaa ttaatcgaca atgactatat
      121 caaaggcgtg caaggtatcg ttcggcaatt cgaaaagacg ttggagcagc tcggtgtgca
      181 gcgtattaaa accgttggtg agccatttga tccacgcgcg catgaagccg tcagcatgga
      241 agaaggcggc agcggccaag aaatcgttag cgaagaatta caatcagggt acatcctggg
      301 tgatgatgtt attcgccacg ccatggtgcg cgtaaaggtg tagcaaaact cccacaaggg
      361 tataatagag tgagcgcctt aatggcgccg tttcaaaggt acactcatga gcacagaaac
      421 caccacgcag cccagccctg atcaagacac ggtagtgctg ctattgcagg tgccgcggac
      481 taatgacaag aaagagctgg ccgccgagca gatgttcgct tcgctccatg gcctgctgac
      541 atttccttcc caaaaaatgt ttcagcccga ccggcgggtg cgagtgagtt ttgaaatagc
      601 tgtgattaat cgccgtatcg gcttctatgt gtgcgtaccg cgctacctgg tcggtttcgt
      661 ggaagaacaa atctacgctc agtatccaat ggtgcaaatt tctgaagtag ctgattacgc
      721 gcaggctgat gaagttcccc aaggttcctt agtaacggaa atgaaattgt cagcaaacga
      781 tgccttgccg atcaagactt tccagagttt tgaggtcgat cccctggcag cgattacggc
      841 cacgctggcc aaattcaccg aagacgaaca ggcttgggtc cagcttatcg tgcaacccgc
      901 ttccaaaaac tggcacaaga aaagcgagcg ttacatcggc accttgcgtg gcggtcgtgg
      961 cggcggttca ctagcgggtg ggctttttag tgctctctgg gcgccgccgg acaatagtgg
     1021 cacggcagcc cccaagttaa ccgagtatga tcaagcccga gccagcggtg ccgaggataa
     1081 gagccataag ctggcttttg aaaccagctt aaggattgtc tataagggca atgtcagccc
     1141 gcaggaggct gagctgcgcc tgcagtccat tatcgccagt tacaagcagt tcaatactac
     1201 ctatctgaat ggtttccagc agaccaagtt ggcgagtgac gccgcactcc tggagcgtta
     1261 ccgaaaacgg gccttgggca agcgcccgct gctcttgaat atcgaagaag tggctacgct
     1321 ataccacttg ccacacacca gcgtggagac gccctatatt ctctgggctt ccagccagac
     1381 cgccgagccc ccggccaatc tgccgatagt aaacgagacc aacatggacg ttgtcagtcc
     1441 gattgcagtg gctaatttcc gtggacataa cgcggcgttc ggcatgccgc gcggcgaccg
     1501 aggccgacac ctttatattc taggacagac gggcgttggt aaatcaggct tactagagct
     1561 gctcactatt tcggaaattt ccaatcctta tggctttgct gtcattgatc cccacggcga
     1621 ctatgcgttg agcgtcctaa agcgtatccc ggcttcgcgg gcccaggacg tgatttactt
     1681 caacccagcc gacaccgatt tcccaatcgc ttttaaccca atggaagtct ccgatccaaa
     1741 gttgcgcacg cacaccgtgt cggagctggt tggtgtcctg aagcgtatgt ttgattcctg
     1801 gggtccgcgc ctcgagtaca tcttacgcta ctgtttactg gcgctgctgg agtatccaga
     1861 agcaaccatg ctcgacatta cccgcattct caccgacaaa tccttcagag ggcaagtcct
     1921 gcaatacgtc accgatccgg tagtgcgtaa cttctggacc gtagagtttg ccacctggaa
     1981 tgaccgtttc gcggctgaag cggtggcgcc ggtgctgaat aaagtaggtg ctttcacagc
     2041 taatccctta gtgcgaaata ttatcggcca accaaagagc agctttaata tccggcaaat
     2101 catggatgag cgcaaaatac tgattgttaa tttgagccgg ggcctggtgg gtgaagacaa
     2161 cgccgcgctg ctgggg
//
LOCUS       DATNGP010000022         4706 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_266942, whole genome shotgun sequence.
ACCESSION   DATNGP010000022 DATNGP010000000
VERSION     DATNGP010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4706
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_266942"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(134..574)
                     /locus_tag="VN778_00545"
     CDS             complement(134..574)
                     /locus_tag="VN778_00545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49504.1"
                     /translation="MKRRLFYVALAGYMVTSYLDVRLLQLSSLTLGENVLRGVGVVIL
                     ALLWLASVLILSAISLRAWLKWLGSGHLGSRTQMFSRVGVLGLIGMALAYVAVSGLVS
                     GDLPVWVYIADLLVPILFFGQAGKIYLKWRTVHATSKPNGPAPS"
     gene            678..2612
                     /locus_tag="VN778_00550"
     CDS             678..2612
                     /locus_tag="VN778_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HXR49505.1"
                     /translation="MTAQEIRKAYLDFFVARGHKVIPRAQLVPYNDPTTLFTGSGMQP
                     LIPYLLGEPHTEGQRLVDSQTCLRAQDIEEVGDNRHTTFFEMLGNWSLGDYFKKEQIP
                     WLFEFLVDQVGLDPKRLYVTCYLGNKELNIPKDNESAAIWQDLFADKGLETNTADIGS
                     EAAGYERGIKPGERIFFYDGKKNWWSRSGAEADTPIGDPCGPDTEVFFEFEHVQHDPK
                     WGAHCHPNCDCGRFLEIANSVFMAYKRTESGFEPLAKPNVDFGGGLERIAVAVIDDPD
                     VFKISLLRPIIIKLEAVSGKNYGSHTESMRVIADHLRAATFLAVDGVKPSNKEQGYVM
                     RRLLRRAIRFAFELGIEQNFLEQIVPVIADLYHDDFPEVASKREEVVEILVKEEKVFR
                     QTLRKGLQTLPKILGASADFSERGESNQSFVVGGDEIFLMYDTYGFPIELTVEEVFKH
                     PDWNLDPDWQKHFNIKMEEQRQRSQTAAKGTFKGGLGGHTMQHKKYHTATHLMYQALR
                     DVLGDHVVQHGSNITEERLRFDFSHSQKMTPEEIKRVEEIVNEQIAKDLKVTFEEFPT
                     KVAIEEKGALGQFGDRYGDTVKVYRMQAEGADKPFSFEICGGPHVDHTGQLAEDGKKF
                     KIIKEESSSSGVRRIKAVLA"
     gene            2666..3145
                     /locus_tag="VN778_00555"
     CDS             2666..3145
                     /locus_tag="VN778_00555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49506.1"
                     /translation="MNGAEGSHLQLVWDNSKGDAFFPDPGSPKLPAGGALSEQEQRPS
                     RRPEDMSAEEIRLWWVFPGDFDEVISDPGRRGRAGRAYRILKHLFEPQATIIEGEVAL
                     PLSQLAQAVVPRDPENPRSTLLDQFVGINAVSTVVEDAQKVVDYKASQIEAADNSSG"
     gene            3209..4540
                     /locus_tag="VN778_00560"
     CDS             3209..4540
                     /locus_tag="VN778_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division FtsA domain-containing protein"
                     /protein_id="HXR49507.1"
                     /translation="MLDKFKSVGLKAVQQAREGAGKAAANARAKTGKPDEQYLVALDI
                     GTEFVKALVGKVGEDKTTVEIIGVGRCHQALTDMQAGAIADIAAVVENCDKALNQAEE
                     QAGVSVRTAIIGIAGELVKGTTTTVRVARKESSKPLDIAEMEKIINLVQERAQNKAKQ
                     QLAWELGGKEVDVRLVNSALVSIEIDGYPVTNPIGFQGKDVVVQLYTAFAPMIHIGAL
                     EKTAQELDLDLLAVAAEPFAVARAVIGNNPNQNLSAILMDVGGGTTDIAVINDGGVQG
                     TKMFGIGGRAYTRAVERDMGVEFDEAEQLKIGLSTNQAPTQKRPAIEKALGKTADVWI
                     GGIELALSEFTKLDHLPHRMFLCGGGSSLDLLMDRLEHTEWYRSLPFTRKPSVHHIRP
                     EQVAGIEDHTGDVTDHTFITAMGLLRVGMDTMQFSSGGGTIRDKIDKMLSV"
BASE COUNT         1185 a         1154 c         1330 g         1037 t
ORIGIN      
        1 tttctcccca tttgccactc acgatcccat ttgctaggac gatgccaaca gccagcatac
       61 agacagccca ggcccattcg taaagcggta ttccgtcaaa tgtcatcagg ggcttcctct
      121 aggtagcagc tgtctagctt ggtgccggac cgttgggctt tgaggtggca tggacagtgc
      181 gccacttcag gtaaatcttg ccggcctgcc cgaaaaacaa aatcggcacc aacaagtccg
      241 cgatatacac ccaaactggc aggtctcccg acacgagtcc agaaacagcc acgtacgcaa
      301 gcgccatccc gataagtccc aagacgccta ccctggagaa catctgggtg cgtgaaccaa
      361 gatgaccact cccgagccat ttaagccacg cgcgtaagct gatagcactc aggatcaaga
      421 ctgatgccag ccacagcagg gcgaggatta ccacgccaac cccgcggagc acattctccc
      481 cgagagtgag actggacagt tgtagaaggc gaacgtcgag atacgatgtg accatatagc
      541 ccgccagcgc gacgtaaaat agacggcgtt tcatttgtta acgaactccc ttcacgggtt
      601 gagtttcgac gaactgaggt aattatataa ttttggctat attagtcaat gtgctgacat
      661 ctgttacaat agaggcgatg acagcgcagg aaatccgtaa ggcttattta gattttttcg
      721 tggctcgcgg gcacaaggtg atcccgcggg cgcagctggt accgtataac gatccgacga
      781 ctttgtttac cgggtcgggc atgcagccat tgatcccata tttgttgggc gaaccgcata
      841 cggagggcca gcggctggta gatagtcaaa cttgcttgcg ggcgcaggac atcgaagaag
      901 tcggcgacaa tcggcacacg acattttttg agatgcttgg taactggtcg ctcggcgatt
      961 atttcaagaa ggagcagatt ccgtggctgt ttgagtttct cgttgaccag gtgggactcg
     1021 atcccaagcg tttgtatgtg acatgctatc ttggcaacaa agaactgaat attcctaaag
     1081 acaacgagtc ggccgcaatc tggcaggatt tgtttgcaga caaaggcttg gagacgaaca
     1141 ctgccgacat tggctcagag gccgccggtt atgagcgcgg gatcaagccc ggtgaacgca
     1201 tcttttttta tgacggcaaa aagaactggt ggagccgcag cggggctgaa gctgacacgc
     1261 cgattggcga tccttgtggg cctgacactg aagtgttttt tgagtttgag cacgtgcagc
     1321 acgatccgaa atggggcgca cactgccatc ctaactgcga ctgcggccgt ttcctcgaga
     1381 tcgccaacag tgtcttcatg gcttataagc ggaccgaaag tggcttcgag ccgctggcta
     1441 aaccgaatgt cgactttggc ggcggtttgg agcgcatcgc cgtggcagtc atcgacgacc
     1501 ccgacgtttt caaaatcagc ttactgcggc cgatcatcat caagctggaa gcagtctccg
     1561 gcaaaaacta tggcagccac accgagagca tgcgcgtcat cgccgaccat ctgcgggcag
     1621 ccaccttcct cgccgtcgac ggcgtcaaac ccagcaacaa agagcaaggc tacgtcatgc
     1681 gccgcctgct gcgccgggcc attcgcttcg ccttcgagct cggcatcgaa caaaatttct
     1741 tggagcagat cgtaccggtc atcgccgatt tgtaccacga cgattttccc gaagtcgcca
     1801 gcaagcgcga ggaagtagtc gagatattag tgaaggaaga aaaggttttc cgtcaaactt
     1861 tgcgcaaagg cctgcagacc ttacctaaaa ttttaggggc gagtgctgat ttttccgaaa
     1921 gaggcgagtc aaatcaatca tttgttgttg gtggagacga aatcttctta atgtatgaca
     1981 cctacggctt tccaatagag cttacagttg aggaagtatt taaacatcct gactggaatt
     2041 tagatccgga ctggcaaaag cacttcaata ttaaaatgga agagcagagg cagcgctcgc
     2101 agacggcggc caaaggaaca ttcaaaggcg ggctcggtgg gcataccatg cagcacaaaa
     2161 agtaccacac ggcgacgcat ttgatgtatc aagcgctacg ggatgtactc ggtgatcatg
     2221 tggtgcagca cggctcaaac attactgagg aacgactacg tttcgacttc agccattcgc
     2281 agaagatgac gccggaggag atcaagcgag ttgaggaaat tgtgaacgag cagattgcta
     2341 aggacctgaa agtaacgttt gaggaatttc caacgaaagt agctattgaa gaaaaggggg
     2401 cgctcggcca gtttggcgat cgctatggcg acacggtgaa agtttatcgc atgcaagcgg
     2461 aaggagcgga taaacctttc agctttgaaa tctgtggcgg cccgcacgtc gatcacactg
     2521 gccagctcgc cgaagatggc aaaaaattca aaatcatcaa agaagagtcg tcatcgtccg
     2581 gtgtccgtcg gattaaagcc gtgctcgcct agctgctgac cacactggcc agccttttgt
     2641 tgctatttat ggtataatag tagttatgaa tggagctgag ggttctcatc tgcaattggt
     2701 ttgggacaat tcgaagggcg atgccttttt ccctgatcct ggctcaccta agctaccggc
     2761 tggcggagct ttaagcgagc aagagcaaag gccttctagg cggccggaag acatgagtgc
     2821 cgaagaaata aggctgtggt gggtctttcc gggagacttt gatgaggtta tctctgatcc
     2881 tggccggcgt ggtcgggcag gacgtgccta ccgtattctc aaacatctct tcgagccgca
     2941 agcgacaatt attgaggggg aagtagcttt gcctttaagc caacttgcgc aagcggtggt
     3001 cccgcgcgat cctgaaaatc ctcggtctac actgttagat caatttgtgg gaattaatgc
     3061 tgtttccacg gtcgtggaag atgcgcaaaa agttgttgat tacaaagcga gccaaattga
     3121 agcggccgac aattcatccg gctagcgatc tttgcattga gcagtttccc agccttttct
     3181 aaggtagtaa tatgctaaaa taatgcatat gcttgataaa tttaaaagcg tgggcctgaa
     3241 agcggtccag caggcccgcg agggtgccgg caaagcggcg gccaatgccc gggctaagac
     3301 aggcaagccg gacgagcagt acttggtagc gctcgacatc ggcaccgagt ttgtcaaggc
     3361 tttagttggt aaagtcggcg aagataagac gaccgtcgag attattggtg tcggtcgttg
     3421 ccatcaggcg ctgactgaca tgcaagcggg cgctatcgct gacattgccg ctgtagttga
     3481 gaattgcgac aaagctctca accaagcgga ggagcaagca ggtgtcagtg tgcggacggc
     3541 gattatcggt atcgctggcg aactagtgaa gggcacgacc accacggtac gcgttgcgcg
     3601 caaggagtcg tcgaagccgc tggatattgc tgagatggag aaaattatca atttggttca
     3661 ggagcgcgcg cagaacaaag ccaagcaaca gttggcttgg gaactcggtg gcaaagaagt
     3721 cgatgtgcgg ctggtgaaca gtgcgctggt gagcattgaa attgatggct atccggtgac
     3781 aaatcccatt ggattccaag gcaaggatgt ggtcgttcag ctctatacgg cgtttgcgcc
     3841 aatgattcat attggggcgc tcgaaaaaac tgctcaggag cttgaccttg atctgttagc
     3901 ggtcgctgcc gagcccttcg ccgtggcccg cgcggtaatt ggcaacaacc ccaaccagaa
     3961 tttaagcgct atcctgatgg atgtcggcgg cggcacgacg gacattgccg ttattaatga
     4021 cggtggcgtg caggggacaa agatgttcgg catcggcggc cgggcttata ccagggccgt
     4081 ggaacgcgac atgggcgtag aattcgatga agccgagcaa ctcaagatcg gtcttagtac
     4141 caatcaagcc ccgactcaga agcgcccggc aatcgaaaaa gccctcggta aaacggccga
     4201 cgtttggatt ggcggtatcg agttggcact gagcgaattt actaagcttg accacttgcc
     4261 gcatcggatg tttctgtgcg gtggcggctc ctcgcttgat ctgttgatgg ataggctgga
     4321 acataccgaa tggtaccgta gcctgccgtt tacccgaaag cccagcgtgc accatatacg
     4381 cccagagcag gtggctggta tcgaagatca cactggtgac gtgaccgatc atacctttat
     4441 cacggccatg ggtttgctac gggtcggcat ggatactatg caattttcaa gcggtggtgg
     4501 tacgattcga gataaaattg ataaaatgtt aagcgtataa catggcagcg acagacaaag
     4561 acaccatcta cattgatatc gacgacgaga tcaccggtat catcgataag ctgaacgcca
     4621 gccccggcaa agtcgtggcg ctggttttgc ccaagcgggc cgccgtcttt cagagtatcg
     4681 tgaatatgaa gctgctgaag cgggcg
//
LOCUS       DATNGP010000023         6757 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_4306655, whole genome shotgun sequence.
ACCESSION   DATNGP010000023 DATNGP010000000
VERSION     DATNGP010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6757
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4306655"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..509
                     /locus_tag="VN778_00565"
     CDS             <1..509
                     /locus_tag="VN778_00565"
                     /inference="COORDINATES: protein motif:HMM:NF023227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4113 domain-containing protein"
                     /protein_id="HXR49508.1"
                     /translation="TSQISVVEAAIASLSARATFRLRKEQLLARSATVFLSTNRHKPG
                     YQRLSRSINFRTPTADTGLITAQLMAALEPIFNTQASYHRTNVLLHNLISQRALQVDF
                     FGEINVASSQAAIARLSAFDALNIRYGRNTIRYAAEDLSDVWEPKHGYRSPRYTSAWD
                     ELPVAHVY"
     gene            514..942
                     /locus_tag="VN778_00570"
     CDS             514..942
                     /locus_tag="VN778_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012693541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HXR49509.1"
                     /translation="MKLHIITIGQPKLDYARSGWQEYEARLRHYHQFRVTHLPDKQND
                     TKHILEAAGSAYKVALEVTGQQFSSHDLALFLEKQALAGKEATFIIGGPTGLPPEVLK
                     AADLHWSLSQLTFPHDLAMVILLETLYRASTINAGHPYHK"
     gene            complement(980..1228)
                     /locus_tag="VN778_00575"
     CDS             complement(980..1228)
                     /locus_tag="VN778_00575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49510.1"
                     /translation="MRLHQGMVIFRPMGGELIIHHMGELEDFGQMELLRKEPSAEVAA
                     NLDDLAMQGFVKKEDDGNSPTYTLTKRGYDFLRASSKQ"
     gene            complement(1247..1933)
                     /locus_tag="VN778_00580"
     CDS             complement(1247..1933)
                     /locus_tag="VN778_00580"
                     /inference="COORDINATES: protein motif:HMM:TIGR02391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02391 family protein"
                     /protein_id="HXR49511.1"
                     /translation="MLGTKAEEVFELGRLNGKSSIIVPDKAEMQRRNTERYHHDLQTA
                     RHQLKAISSFLKSNLAQDNEPKSEPVQAGTLEALHSTIQEKCSKLYLSTNYSEAAEKG
                     LKSVDDRLRETTGFDKGNEAVGKAGLYFLGAAAPHVDKDFQQAIQLLLMSIHLFRDEK
                     AHTSDAKIDDPVRAYEYLAMCSFAMHFLDNCEIRKREPRPKPPKATTVSPQKKLVTFS
                     YDELSPIQIQ"
     gene            2095..2823
                     /locus_tag="VN778_00585"
     CDS             2095..2823
                     /locus_tag="VN778_00585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49512.1"
                     /translation="MQFIVPVLREMASWGPDDPDFTRIYSLAKELAKYLDASDEDKDE
                     ADFAADRILEKLDSALAHYQKINAPNFASLNTTQQHAEYEGLYGALWSTYKDRMQKYL
                     KKCGVNIGFIFFNDVRFEQKATEFAIQDPDLGQFVDFLRKQRSGWQNDLVKNRNAHEH
                     DGDLRSGIPDYDNPMDAKRLFAQVCWTMESLIGNLVSWRLKPEWNVIDTAPNSTVFNR
                     QPRFIVEHAIMTAQREKNEQRSSP"
     gene            complement(2912..2988)
                     /locus_tag="VN778_00590"
     tRNA            complement(2912..2988)
                     /locus_tag="VN778_00590"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2952..2954),aa:Glu,seq:ctc)
     gene            3227..4441
                     /locus_tag="VN778_00595"
     CDS             3227..4441
                     /locus_tag="VN778_00595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXR49513.1"
                     /translation="MTGELADETTRFAAIIRTITDGVILIDNQQVIQMINPAGAAICG
                     WPVDEATSLSIQTVMKLVTDKGEAIGSPDNPFNRIFSQTETLKDNNAFVMNREGKQVS
                     IALSISPLLDQDQQVTAAVVVFRDVSEERAQEKQRADFISTASHEMRTPVAAIEGYLA
                     LALNDKVSTIDNRARNYLEKAHASTQHLGQLFQDLLTSAKAEDGRLTSHPTVVELGAF
                     LEQLTNDLRFAAEKKGLAMEYIVGSNHVIDATKTTGGERTIRPFYYVHVDADRLREVI
                     TNLFDNAVKYTPEGRVTLGLTGNDQVVQCYIQDTGPGIPAEDVPHLFQKFYRVDNSAT
                     RTIGGTGLGLFICRKIIELYNGRIWVESEVGKGSTFYINLPRLSTEKANQLQAQAASS
                     TELKPIETLASP"
     gene            4503..5963
                     /locus_tag="VN778_00600"
     CDS             4503..5963
                     /locus_tag="VN778_00600"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXR49514.1"
                     /translation="MAKVMLVEDDNNLREIYEARLMAEGYDIVSAKDGEEALVVAKAE
                     KPDLIISDVMMPKISGFEMLDILRNTDGLKDVKVIMLTALGQSDDQQRADRLGADRYL
                     VKSQVTLEDIVKVSHELLEGTDGTAAAPPPAAPTPPSAPTPPASPPAEPPTAPPTTDS
                     STAASAAPQATPVSAVPTTAVPATPAPTTIPVAPAPPAAGTAASDDKLMADVVDKLLA
                     STDDKQTPADPATATTAPPVTTNTSSADNSSTTEVAAAAPDVVAEEEVSSQVPVAHKK
                     VIEPLDSPQKKDLDALLAEEEAKEAAAAPPSPPTAVIATAEAGQKVEPLTAEVAPPAS
                     PPAAATTPATQAPTAEPAPPASPTDNSAQTTAQEEASVEAKIEDFVAGATTEATAPVT
                     IGDQALALDITAPAPAVPTPEQPKIGKIMSATTPTSLAATADAPSGAVSAPANPAPAV
                     PAASAITGQASGMSLPTNAAGEPAAPALGDPNSIAL"
     gene            6004..6624
                     /locus_tag="VN778_00605"
     CDS             6004..6624
                     /locus_tag="VN778_00605"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HXR49515.1"
                     /translation="MSRRAADTVIAQDRVTINGQVAELGDDVNGEAIVRLDDKPLRLP
                     EQPLTIMLHKPVGFVCSRNGQGSSTIYDLLPPQYRELKPVGRLDKDSSGLLVLTNDGQ
                     LAHELTHPRYTKEKIYEIALDKPLVLKDKTHVEQGVQLEDGISALALAPRDTPSQQWH
                     ITMTEGRNRQIRRTFAALGYTVTRLHRTKFGPYDLGNLPPGESRRV"
BASE COUNT         1747 a         1873 c         1611 g         1526 t
ORIGIN      
        1 ataccagcca aatttcagtc gtagaggcag ccatagccag cttgagcgcc cgggctactt
       61 ttcgcctgcg aaaagagcag ctgctggctc gcagcgccac ggtattcctt agcaccaacc
      121 gtcacaaacc aggctaccag cgccttagcc gcagtataaa ttttagaacc ccgacagccg
      181 ataccggctt aattaccgcc cagctgatgg ctgccctgga gccgattttt aacactcagg
      241 ccagttatca tcgaaccaac gtgctactcc ataacctgat cagccagcga gccctacaag
      301 tcgatttctt tggggagatc aacgtcgcca gcagccaggc cgctatcgct cgcctgagcg
      361 ccttcgacgc cctcaacatc cgctacggac gcaacaccat tcggtatgcc gccgaagatc
      421 tgagtgacgt ttgggagccc aaacacggtt accgcagccc gcgctacacc tctgcgtggg
      481 acgaactgcc cgtggctcac gtatactaac agcatgaagt tgcacattat taccatcggt
      541 cagcccaagc tcgactacgc ccgcagcggc tggcaagaat acgaagctcg cctcagacat
      601 tatcaccagt ttcgggttac tcatttgcca gacaaacaaa acgataccaa gcatatcctc
      661 gaagccgccg gctcagccta caaagttgcc ctcgaagtca ccggccagca attctctagc
      721 cacgacctgg ccctatttct agaaaagcaa gctttagcgg gcaaggaagc caccttcatc
      781 atcggcggtc ccactggttt accgccggaa gtgctcaaag ccgctgatct ccattggagc
      841 ctcagccaac tcacattccc acacgacctg gccatggtaa tccttctcga gaccctctac
      901 cgcgccagca cgatcaacgc cggccatcca taccataagt agctacacaa agagcaatta
      961 tttactcaga accgaagggt tattgttttg agctcgccct gagaaaatcg tagcccctct
     1021 tcgtaagcgt atatgtcggg ctattaccat cgtcttcttt ctttacgaag ccttgcatgg
     1081 ctaggtcatc aagatttgcc gcaacttcgg cactaggttc tttcctgagg agttccattt
     1141 gtccgaaatc ttcaagctcg cccatatgat gaattatcaa ttcaccgccc atgggtcgaa
     1201 aaataaccat accttggtgc agcctcattt ttcccattat cttcagtcac tgtatttgta
     1261 ttggactaag ttcgtcatat gaaaaagtaa caagcttctt ttgcggcgaa acagtagtgg
     1321 ccttgggcgg cttggggcga ggctccctct ttcgtatttc gcaattgtct aggaaatgca
     1381 tagcaaagct gcacatggcg aggtattcat atgctcgaac tgggtcatct atcttggcgt
     1441 cggatgtatg tgctttctca tctctaaaaa gatgaattga catcagcagc aactgaattg
     1501 cttgctgaaa atctttgtcc acgtgcggtg ctgcggcacc taggaagtac aaaccagctt
     1561 tacctacggc ctcgttacct ttgtcgaaac ctgtggtctc tcttaagcgg tcgtcgactg
     1621 atttaagccc tttttctgca gcttcagaat agttagtcga aagatataac tttgagcatt
     1681 tttcttgaat agtagaatga agggcttcaa gcgtaccagc ttgaacaggt tcggactttg
     1741 gctcgttatc ttgagctaag ttcgacttta agaatgatga aatagcttta agctggtggc
     1801 gggcagtttg tagatcgtga tggtatctct cggtattgcg gcgttgcatt tctgctttat
     1861 caggcacgat aatagagctt ttaccattta atcgtcctaa ttcgaaaacc tcttcggcct
     1921 tagtcccaag catttgcttt gtaagtacat tacatgagtg tgccactctt caaagttctc
     1981 tcggtcttcg gacttctcca gtacatcaat ttggcttatg agtgagtcca gccgctctat
     2041 ttctttagca tccatatata gatagtacca ccacatgtgg cctataatac gagtatgcaa
     2101 ttcattgttc ctgttttacg cgagatggcc agttggggtc cggacgaccc agattttaca
     2161 cgtatttatt ctcttgccaa agagctggca aagtatctcg acgcaagcga tgaggacaag
     2221 gatgaagccg atttcgccgc tgatcggata ctggagaaac tcgattcggc actagctcat
     2281 tatcaaaaaa tcaatgcccc gaactttgca tcattgaata caacccaaca acacgccgaa
     2341 tacgaaggtt tatatggtgc gctctggtct acctataaag accggatgca gaaatattta
     2401 aagaaatgtg gcgtaaacat cggcttcatt tttttcaatg atgtccggtt tgagcaaaaa
     2461 gccactgagt ttgcaattca agacccagac ctggggcaat tcgttgattt ccttcgaaaa
     2521 cagcgtagcg gctggcaaaa tgacctagtt aagaatcgca atgcgcacga gcacgatggt
     2581 gatttacgtt ccggcatacc tgactatgac aatcctatgg atgctaaacg attatttgca
     2641 caggtttgtt ggacaatgga atctcttatc ggcaacttag taagctggcg cctaaagcca
     2701 gagtggaatg ttattgatac tgcgcctaac tctaccgtgt ttaacagaca gccacgcttt
     2761 atcgtagagc atgctatcat gactgctcag cgtgagaaaa acgaacaacg ttcatcacca
     2821 taaatgaatc aagctcaatt ctacagaggt agaaatcaaa aaagagctcc gagcagtgaa
     2881 taatgtaact cttttcatct gatatctacg atggtgaccc ctgcgggatt cgaacccgcg
     2941 atcttctgga tgagaaccag atatcctgga ccactagacg aaggggccaa aagttttcag
     3001 gctcggatat cttaccacat ctgttagccg tcgtctacga cagggaaggt gtaagcatta
     3061 gaggcattgc aatgtacctc tgaaacatag ataataaatt aaaacaaact atgtattgcg
     3121 tccctttgat cggcagtaat gggtgggcct ggcgaactgc atcaggggag tattaggtat
     3181 gtttggttta ggtaagcctt ctatcccaaa taaagaagca aaccatatga ctggcgagtt
     3241 ggccgatgag acaactcgtt tcgcggccat catcagaact attaccgacg gtgttatttt
     3301 aatcgacaat cagcaggtca ttcagatgat taatccagct ggggctgcca tttgcgggtg
     3361 gccggttgat gaagcgacta gcctcagcat ccaaacagtt atgaagctcg tcacagacaa
     3421 gggcgaggct atcggctcac cggacaatcc cttcaatcgc atctttagcc aaacagaaac
     3481 tctcaaagat aataacgcct ttgtcatgaa tcgtgaaggc aagcaggtct cgatcgctct
     3541 gagcatttcg ccgctgctcg accaggatca gcaggtcacg gccgctgtcg tcgtatttcg
     3601 tgacgtcagc gaggaacgtg cccaggaaaa gcaacgcgcg gacttcatta gcaccgccag
     3661 ccatgagatg cggacgcctg tggccgctat agagggttat ctagcattgg cgctcaacga
     3721 taaggtttcg actatcgaca atcgagcccg caattacctg gaaaaggctc atgcctcgac
     3781 ccaacacctt gggcagctgt tccaggatct gctgaccagc gctaaggctg aagatggacg
     3841 tttaactagc cacccgacgg tggtggaact gggcgctttt ctcgagcaac taacgaacga
     3901 tttgcgcttt gcagccgaga agaagggttt ggcaatggaa tacattgtcg gctccaatca
     3961 tgttattgac gctacgaaga ccacaggcgg cgaaaggaca atccggccat tttattacgt
     4021 ccacgtcgat gccgatcgcc tccgcgaagt tattaccaat cttttcgata atgccgtcaa
     4081 atacacgcct gaaggccggg taacgctcgg tcttaccggc aatgatcaag tagtccagtg
     4141 ttacatccag gacactggac caggtatccc ggccgaagat gtcccgcatc tattccaaaa
     4201 gttttaccgc gttgataact cagccactcg taccatcggt ggcacggggc tcggtttatt
     4261 catttgccgc aagattatcg agctctacaa tggccggatt tgggtcgaaa gcgaggtcgg
     4321 caagggcagt acattttata tcaacttgcc gcggctgagc accgagaaag ctaatcagtt
     4381 acaagctcaa gccgcctcga gcactgagct caaacctatc gagacgctag cttcaccgtg
     4441 agacatgata caatgaaagt aaacctaacc ataagcaaga aacaagaagg acaccgatca
     4501 ttatggcaaa agtaatgctc gtcgaagacg acaacaatct gcgcgagatc tatgaggccc
     4561 gcctcatggc tgaagggtat gacatcgtct cggcaaagga tggcgaggaa gcgctcgtcg
     4621 ttgccaaagc agagaagcct gatttgataa tctccgatgt tatgatgccc aaaatcagcg
     4681 gctttgagat gttggacatc ttgcgcaata ccgacggctt gaaggacgtt aaagtcatca
     4741 tgttgaccgc acttggacaa agtgacgacc agcaacgggc cgatcgtctg ggtgccgacc
     4801 gctacctcgt caaatcacaa gtcaccctgg aagatatcgt caaagtatcc catgagcttc
     4861 tggaaggcac tgatggcaca gctgcagcgc ctccgcccgc agccccaaca ccaccttccg
     4921 cgccgacacc acccgccagt ccgccagccg aaccaccaac agcgccacct actaccgata
     4981 gtagtaccgc tgcatctgca gcgccccagg caactccagt cagcgccgtt cccactacag
     5041 ccgttccagc cacgccagct ccgacaacta ttccagttgc gccggcccct cctgccgctg
     5101 gcaccgcagc gagtgatgac aaactgatgg ccgatgtggt tgataagctg ctggccagta
     5161 ctgacgataa gcagacgccc gcagatcccg ctacggccac caccgcaccg cccgtaacaa
     5221 ccaacacgtc gtcagctgac aactcttcga ccaccgaggt cgcagctgca gcccccgacg
     5281 tagtcgccga agaagaagta tccagccaag taccggtagc tcacaaaaaa gtgattgaac
     5341 cactcgacag ccctcagaaa aaagacctcg atgctttgct tgccgaagaa gaagccaagg
     5401 aagccgctgc ggcgccccct tcgccaccaa ctgccgttat cgcgaccgct gaagccggtc
     5461 agaaagtcga gccgttgaca gcggaagtag cgccaccggc cagtccgcca gcagcagcga
     5521 ctactcccgc cacccaggca ccaaccgctg aaccagctcc accagcctcg ccaactgata
     5581 actcggccca gacaactgct caagaagaag cgtcggtcga ggctaaaatc gaagattttg
     5641 tcgccggcgc taccaccgag gccacagcac cagtgaccat cggcgaccaa gcactagcac
     5701 ttgacattac cgcaccagct ccggctgtgc caacccccga gcagccgaaa ataggcaaaa
     5761 tcatgtccgc caccacgccg acctcgctag ctgccactgc cgatgcacct tccggtgcgg
     5821 tctcagctcc cgccaaccct gcgccggcag ttccagctgc tagtgccatt accggccaag
     5881 cgtcaggcat gagtttgccc accaatgccg ctggcgaacc agccgctcct gctttaggcg
     5941 atccgaacag cattgctctc tagctacatg cgtatcaaca aatttgttgc gggagccacc
     6001 ggaatgagtc ggcgagctgc ggatacggtc attgcgcagg acagagtaac gattaacggc
     6061 caggtggctg aattaggtga cgacgtgaac ggggaagcca ttgtccggct ggacgataag
     6121 cccctccggc tgcccgagca acccctgacc atcatgctcc ataagcctgt cggtttcgtc
     6181 tgctccagga atggacaggg cagctcgacc atttacgatt tgttgccacc gcagtatcgc
     6241 gagctaaagc ccgtcgggcg cctcgacaag gactcctctg gcctcctggt gcttaccaat
     6301 gacggccagt tagcccacga gctaactcat cctcggtata ccaaggaaaa aatatatgag
     6361 atagcacttg ataagccgct ggtgctcaaa gataaaaccc atgtagagca aggcgtccag
     6421 ctcgaagatg gtatcagcgc gcttgccctt gctccgagag atacgcccag ccagcagtgg
     6481 cacattacga tgacagaagg ccgtaaccgg cagatccgtc gtacctttgc ggcgctcggc
     6541 tacaccgtaa cgagactgca tcgaacaaag ttcgggccat atgatctggg taatttaccc
     6601 ccaggagagt cacgacgcgt ataaggcctc ttgctgagag gttagaaatc tttaggcagg
     6661 ataagatcag cgttaagagc agtaacgaat ttatcgaccc attcttccat gggcgctcgt
     6721 tgaagaattt tggcatcttt tctcatattg gcgctca
//
LOCUS       DATNGP010000024         3687 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_2579269, whole genome shotgun sequence.
ACCESSION   DATNGP010000024 DATNGP010000000
VERSION     DATNGP010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3687
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2579269"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..428)
                     /locus_tag="VN778_00610"
     CDS             complement(<1..428)
                     /locus_tag="VN778_00610"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HXR49516.1"
                     /translation="MYSEEAAKIEDVLTPAKTLVIVQADNPDADSLGSALALEQIFGD
                     LGKETHLYCGVDVPTYLRYMAGWDRVNKELPNHFDASVIVDASTMTLFDRLSGSGQQG
                     WLAAKPCLVLDHHETVENVIPFANLVLNDYSRASTGELIY"
     gene            complement(436..1044)
                     /gene="recR"
                     /locus_tag="VN778_00615"
     CDS             complement(436..1044)
                     /gene="recR"
                     /locus_tag="VN778_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HXR49517.1"
                     /translation="MQILPPALNDLIEAFGLLPGVGPRTAERYAYYIFKQPGNAGDRL
                     AETLQSLKAGVGICKKTFALIPAGQGLSELYTDPARDKATIAVVAEPFDIVAIEKTGQ
                     YKGTYHVLGGLVSPIDGIGPEQLHIKELVQRVDEDKVKEIILATNASVEGESTALYIQ
                     QQIGPARKLKITRLARGLSVGVDLEYADQITLGRAIEGRQAI"
     gene            complement(1052..1345)
                     /locus_tag="VN778_00620"
     CDS             complement(1052..1345)
                     /locus_tag="VN778_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015642082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HXR49518.1"
                     /translation="MSRFDQAKMLMRVKKIQGELQKMTIAVEKGDGAVRVEFNGEQKL
                     KRIQIDPEYVDTDDIGQLERWIEEAVREAIQESQKVAAEKMQPMMGALSNLGL"
     gene            complement(1380..2567)
                     /locus_tag="VN778_00625"
     CDS             complement(1380..2567)
                     /locus_tag="VN778_00625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49519.1"
                     /translation="MAKKEKGGLPKGWLLALITAAVCILLLLINRLGSLTGGVSAGEV
                     HAATAPVGWHGIYHNPFYLPLKLARSVAFFLFSAHGQTITRLPNVLFGIMTIAAFIIL
                     VKLWHSTRTAVLASFLFVTSAWVLHASRLASFDVIYLWALPILLLSNVLINRSPDRGL
                     SWYFGLLTMGMLLYVPGMVWLILVNIFFLRHELAEAWAEFSYKQHILFILASLIWLPL
                     LIVNLLRPSQLLVWLGLPTHWPTLKLLLKQFIGVPVHLFIRGPQQPDLWLGRAPILDI
                     FTLVACILGIYFYLRHLKANRTRLLACFAIIGWLLVTIGGPVTFSLLVPLLYLAAATG
                     IAYLLHEWLRVFPLNPLARGLGVTLVTLAVGLACIYNLRAYFVAWPHDQATRATFIYH
                     LRH"
     gene            complement(2584..>3687)
                     /gene="dnaB"
                     /locus_tag="VN778_00630"
     CDS             complement(2584..>3687)
                     /gene="dnaB"
                     /locus_tag="VN778_00630"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015642084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HXR49520.1"
                     /translation="AYLTELTNFVPTASHVEQYADIVAQKAMRRRLIAASKHIGGLGY
                     DESKQLRELVEEAETHLFEISQQHIKQNIVGLETILAESFDRLDELHKDKQKIRGIPT
                     GFKDLDNMLAGLQRSDLVVLAARPSMGKTALALNFAHNVAVQSGEPVLIFSLEMSKEQ
                     LVDRLLSMESGVNAWALRTGNLTDADFEKIGHAMGTLSEAQIYIDDSPSITISDLRTK
                     ARREAHKRPLGLIIVDYLQLMSGGSRFGGEGNRVQEISEISRGLKGVARELNVPVLAL
                     SQLSRSVESRSPQIPQLADLRESGSIEQDADVVAFIYREEYYNPETDRKKLTDIFIKK
                     HRNGPTGGVEMYFDNERQRFRSVENRRSDPFAD"
BASE COUNT          902 a          932 c          932 g          921 t
ORIGIN      
        1 tggtagatta gttcgccggt tgaagcgcgg ctgtagtcat taagcaccag atttgcaaat
       61 ggtattacgt tctccactgt ctcgtggtgg tccagtacga gacatggctt ggcggccagc
      121 catccctgct ggccggagcc gctgagccta tcaaaaagtg tcatagtcga agcatcgaca
      181 atgacagagg cgtcaaagtg gttgggcagc tctttgttaa ccctgtccca gccggccatg
      241 taacgcaaat aagtcggaac gtcgacgccg caatagaggt gcgtctcttt cccaaggtcg
      301 ccgaagatct gttcaagcgc cagggcgctt ccaagcgaat cggcatcggg gttgtcggcc
      361 tggactatga ccagcgtttt tgccggagta agcacgtctt ctattttggc ggcttcttcg
      421 ctatacatgc gtcaattata tagcttggcg cccctcaatg gcacggccaa gcgtaatctg
      481 gtcggcgtac tctaggtcga cacctactga caaacctcgc gctaaacgag tgatttttag
      541 cttacgggct ggccctatct gctgttggat atataaggcc gtagattctc cttcaacgct
      601 ggcattggtg gccaggatta tttctttgac tttatcctca tcgacgcgtt gaaccagttc
      661 tttaatgtgg agctgctccg ggccgatgcc atcaatcggc gacactagtc cgccaaggac
      721 atggtaggta cccttatact gcccggtttt ctcaatggcc actatatcga acggctcagc
      781 cacgaccgca atagttgctt tatcgcgggc ggggtcagta tacagctccg acagcccttg
      841 tccggcggga atcagcgcaa acgtcttttt gcatatgcca acaccggctt ttaatgattg
      901 taatgtctca gcaagcctgt caccggcgtt tccaggttgt ttgaaaatat agtaggcata
      961 gcgctccgca gtgcgcggcc cgacaccagg caacaagccg aacgcctcaa tcaggtcatt
     1021 taacgctggc ggtaaaatct gcatgcttgg cttaaaggcc tagattgctt agggcgccca
     1081 tcatcggctg catcttttcg gcggctacct tctggctttc ctggatggct tcgcgtacag
     1141 cttcttcgat ccagcgctca agctggccga tgtcgtcggt atcgacatat tcggggtcga
     1201 tttggatcct ttttaacttt tgctcgccgt taaactcaac cctaacggcc ccgtcgccct
     1261 tctctacggc tatcgtcatt ttctgcaatt cgccttggat tttttttaca cgcattaaca
     1321 tcttagcttg gtcaaatcgg ctcatatatc ctcccttttc ctaatattat accaatattt
     1381 caatgccgta agtggtagat aaaggtggcc cgtgtcgctt ggtcgtgtgg ccaggctacg
     1441 aaataagcgc gcaaattgta tatgcaggct aatcccacgg ctagcgtcac aagagtgacg
     1501 ccgagcccac gtgcaagtgg attgagagga aaaaccctta gccactcgtg cagtaggtag
     1561 gcgatacctg tggctgctgc cagatataat agtggtacga gcaagctaaa agtgacgggt
     1621 ccgccgattg ttaccaacag ccagccgatg atggcgaaac aggccaagag ccgtgtgcgg
     1681 ttggccttca gatgtcttaa gtaaaaatat atgccgagaa tacaagcgac tagggtgaaa
     1741 atatctaaaa ttggcgccct gcctaaccac aggtcaggct gttgaggacc gcgaatgaat
     1801 aaatgtaccg ggacacctat aaattgcttg agtagcagct taagtgtggg ccagtgcgta
     1861 ggcaggccta accaaactaa tagttggctg gggcggagca gatttactat tagtagcggc
     1921 agccatatca gggatgccaa aatgaaaagt atatgctgtt tatatgaaaa ttcagcccac
     1981 gcttcggcaa gctcatggcg caaaaagaag atattaacca aaataagcca taccatgcct
     2041 ggaacatata acagcatgcc catggttaat aagccgaaat accaggacag accgcggtcg
     2101 ggggaacggt ttatcagcac attactaagc aataaaattg gcagcgccca cagatagatg
     2161 acatcaaaac tagctaagcg gctagcgtga agcacccagg ccgaagtaac aaatagaaaa
     2221 ctggccagca ctgccgtacg cgtgctgtgc cacaatttga ctaagattat gaaagcggca
     2281 attgtcatta tcccgaacaa tacatttggc aggcgagtga ttgtttgtcc gtgcgccgaa
     2341 aaaagaaaga atgctactga gcgcgctaac tttaatggca agtaaaaagg gttatgataa
     2401 attccatgcc aaccaacagg ggcagtagcg gcatgtactt cgccggcgct aacaccaccg
     2461 gtcaagctgc ctaggcggtt tatgagcaat aacaagatac aaacggcagc tgtgattaaa
     2521 gcaagcaacc accctttagg taaacccccc ttctcttttt ttgccatgca gtcattatat
     2581 cagctagtcg gcgaagggat ccgaacggcg attttcgacg gaacgaaaac gttgacgttc
     2641 gttgtcgaaa tacatttcta ccccacccgt cggcccattg cggtgcttct ttataaagat
     2701 gtccgtcagc tttttccgat cggtctctgg gttatagtac tcctcgcggt agataaaagc
     2761 gacgacatcg gcatcttgct cgatagaacc ggattcacgc aggtcggcaa gctggggaat
     2821 ctgggggcta cggctttcga ctgagcggct caactggcta agcgcgagta cgggcacgtt
     2881 aagttcgcgg gctactcctt taaggccgcg ggatatctcg gaaatttcct gcacgcggtt
     2941 gccttcaccg ccaaaacggc tgccgccact cattagctgc aagtagtcaa cgataatcag
     3001 gccaagtggg cgtttgtgcg cttcacggcg ggcctttgtc cttaaatcgc ttatggtaat
     3061 gctagggctg tcgtcgatat aaatttgagc ctcactcagg gtgcccatag cgtgtccgat
     3121 cttttcgaaa tcggcgtcgg taagattacc ggttcgtagc gcccacgcgt tgacgccaga
     3181 ttccattgac agtaagcggt ccaccaactg ctctttactc atttccaggc tgaatatgag
     3241 cacgggttcg ccagattgca cggccacatt gtgggcgaaa ttcagagcca gggccgtttt
     3301 acccatggaa ggcctggctg ccagtacgac taaatctgag cgttggagtc cggccagcat
     3361 gttatctaga tccttaaaac cggtcggtat accccttatc ttctgtttat ctttatgaag
     3421 ctcgtccagc ctgtcaaaac tttcggccaa gatggtctcg aggccgacaa tattttgttt
     3481 gatatgttgt tggctaattt caaagagatg tgtttcggct tcttcaacta attcccgcaa
     3541 ttgtttagac tcatcgtaac cgaggccacc aatatgcttg gaggcggcta tcagacgtcg
     3601 tctcatggct ttctgggcca caatgtcggc atattgctca acatggctgg cggtcggcac
     3661 aaagttagtc agctctgtca aataggc
//
LOCUS       DATNGP010000025         2494 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_392810, whole genome shotgun sequence.
ACCESSION   DATNGP010000025 DATNGP010000000
VERSION     DATNGP010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2494
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_392810"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..781
                     /locus_tag="VN778_00635"
     CDS             <1..781
                     /locus_tag="VN778_00635"
                     /inference="COORDINATES: protein motif:HMM:NF012796.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminoacyl--tRNA ligase-related protein"
                     /protein_id="HXR49521.1"
                     /translation="FVLQTLTEEKVIKQLITENNLQLRAGAFTPVLPPVMIREAVYDQ
                     MDRLEPREDRYHIEGEDDDLWLIGSAEHTLGSMYAGEILPSDELPVRYIGYSTSFRQE
                     AGTYGKDSEGIFRVHQFDKLEMEVFSSAETGRDEHLLLITIQEYLMQQLGIPYRVLMK
                     CTADIGKPNARGVDIEAWLPGQGKYRETHTADYMTDYQARRLKTRYREEEAANLIHTN
                     DATAFALGRTLIAILENGQTKEGKVHIPKVLQPYMGGRTEI"
     gene            839..1201
                     /gene="raiA"
                     /locus_tag="VN778_00640"
     CDS             839..1201
                     /gene="raiA"
                     /locus_tag="VN778_00640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HXR49522.1"
                     /translation="MFQKFEIRGVHTTVNDSLHKYVTKKIGNLDRYISRHARSSAHAE
                     VHLKEMKSKDNNRHACEVTIHLPHQTIVIKEKALNMFAAVDIVEAKLKQQLQKYKDLH
                     GSGKAHRQLFARFRRSEA"
     gene            complement(1211..1879)
                     /locus_tag="VN778_00645"
     CDS             complement(1211..1879)
                     /locus_tag="VN778_00645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49523.1"
                     /translation="MRRVELGISQRPERYEAHDIASLVVNSFRFSRGNAVCQDAILGY
                     IATSFGSCFVLETFPDAVSQAQRKVLSTYNRLHISKNASESLVRSEAEDYTEMLKGFV
                     FYPDKKMRASLLAEGITSQLIDSDYKWLPRTRHAEEKPPLPRPYNSNLVTYGFLDKST
                     GRPEFRAIFDFATRSILGGQLPKSDDLDWAENVVAEHYKYQPIDRVALQARLTDTVLE
                     SAIN"
     gene            1997..>2494
                     /locus_tag="VN778_00650"
     CDS             1997..>2494
                     /locus_tag="VN778_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HXR49524.1"
                     /translation="MNIVLKKILGDPQAKTVKRLRRRVKAVNDLADKYQKMTDKQLKE
                     QTDVLKKRLKKESLDAILPDAFAVVREAATRTLGQRHFDVQLIGGMVLHEGNVAEMKT
                     GEGKTLVATLPVYLNALTEKGVHVVTVNDYLAQRDAGWMGQVYNFLGLQAAVIIADQS
                     FAYDPE"
BASE COUNT          676 a          634 c          629 g          555 t
ORIGIN      
        1 ctttgtgctg cagacattga ccgaagagaa ggtgataaaa cagcttataa cagagaataa
       61 tctgcaactc cgtgcggggg ctttcacacc agttttgccg ccggtaatga tccgcgaggc
      121 ggtgtacgac cagatggatc gcctggagcc gcgcgaagat cgctaccata tcgagggcga
      181 agacgatgat ttgtggctga tcggcagcgc cgagcacacg ctgggtagca tgtacgcggg
      241 cgagatcctg ccatctgatg agctgccagt tcggtatatt ggctactcca cgagctttcg
      301 gcaggaggcc ggcacctacg gcaaggactc ggaaggcatt tttcgagtgc accaattcga
      361 taagctggag atggaagttt tcagtagcgc agaaactggc cgcgatgagc acctgctact
      421 gattaccatt caagaatatc tgatgcaaca attgggcatt ccataccgcg tgctcatgaa
      481 gtgtacggcc gatattggta agccgaacgc gcgcggtgtc gacatcgaag cctggctgcc
      541 cgggcaaggc aagtaccgcg aaacgcacac ggctgactac atgaccgatt accaagcccg
      601 ccgcctcaaa acccgctacc gcgaggagga agctgctaac ctcattcaca ccaatgacgc
      661 cacggccttt gccctcggcc ggacattgat cgcaattctc gaaaatggcc agaccaaaga
      721 aggaaaagta cacataccca aggtgcttca gccgtatatg ggcggccgga ccgagatttg
      781 agttgcgacc cgcgcgtata attaagagta cggtactagt taacaaatag gagggtatat
      841 gtttcaaaaa ttcgagatcc gaggcgtgca cactactgtt aacgattcgc ttcacaagta
      901 cgtcaccaaa aaaattggca atctcgatcg ctacatttcc cggcacgccc gctccagcgc
      961 tcacgccgaa gtccatctaa aagagatgaa atcaaaggac aacaaccgcc acgcctgcga
     1021 agtcaccatc cacctgccgc accaaaccat cgttatcaaa gaaaaagccc taaacatgtt
     1081 tgccgccgtc gacatcgtcg aagccaagct caaacagcaa cttcagaaat acaaagactt
     1141 gcacggcagc ggcaaagccc accgccaatt gtttgcccgc ttccgccgta gtgaagccta
     1201 gcagcctgaa ctagttgata gcagattcga gaacggtatc agtgagacgg gcttgaagtg
     1261 caacacgatc gatcggctga tatttataat gttctgcaac cacattttct gcccagtcca
     1321 agtcgtcgct cttgggtagt tgccctccga gtattgatct ggtagcaaaa tcaaaaatgg
     1381 ccctaaattc agggcgaccg gtgcttttgt caaggaaacc ataagttacg aggttgctat
     1441 tgtacggtcg aggcagaggc ggcttctcct cggcgtgcct ggttcgcggc aaccacttgt
     1501 agtcggaatc gataagttgt gaagttatgc cttcagctag tagtgatgct ctcatttttt
     1561 tgtctggata aaatacaaag cctttgagca tttcagtgta atcttccgct tcagatcgta
     1621 ccaggctctc gctcgcattc ttactgatat gtagcctgtt gtaggtactt aaaaccttcc
     1681 tttgagcttg gctgactgcg tcaggaaaag tttcaagcac aaaacaggac ccgaaacttg
     1741 ttgcaatgta gcccagaata gcatcctggc aaacagcatt acctcttgaa aaacgaaatg
     1801 aatttacaac cagactcgca atatcgtgag cttcatatct ttccggtcgt tgagatatgc
     1861 ctaattcaac ccgtctcata agcaaatatt ataatatata tgcatacaag tcaatgctag
     1921 ctcttggggt caaaagcggt tataattaca tatcgaatag gttcttagtc agataaccgg
     1981 gagataaaac gtactgatga atattgtttt gaagaaaatt ttgggggatc cacaggccaa
     2041 gacggtcaag cgcttacgcc ggcgtgtcaa agccgtcaac gacttggcgg acaaatacca
     2101 aaaaatgacc gataagcagc tcaaagagca gaccgatgtg ctcaaaaaac ggctgaaaaa
     2161 agagtcgctc gatgccattc tgcctgatgc tttcgcggtt gtccgtgagg cggctacgcg
     2221 gacgcttggc cagcgccact tcgatgtcca gctgatcggc ggcatggtgc tgcacgaagg
     2281 taacgtcgcc gagatgaaga ccggtgaagg caagacctta gtggccacgc tgccggtgta
     2341 tctaaacgcg ctgaccgaga aaggcgtgca cgtcgtgacc gtcaacgact atctggcgca
     2401 gcgcgacgcc ggttggatgg gtcaagtcta caactttcta ggtctccaag cagcggttat
     2461 cattgctgac cagagttttg catacgatcc ggag
//
LOCUS       DATNGP010000026         2463 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_1833768, whole genome shotgun sequence.
ACCESSION   DATNGP010000026 DATNGP010000000
VERSION     DATNGP010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2463
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1833768"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..504)
                     /locus_tag="VN778_00655"
     CDS             complement(<1..504)
                     /locus_tag="VN778_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018550247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HXR49525.1"
                     /translation="MPITALDQKTALVIIDLQKGITALSTAHSMQQVISNSAKLAEAF
                     RRHRLPVVLVNVTGGAPGRTESPARGGDRPSDWAELVPELNVQPGDIKITKRTWGAFH
                     NTNLDKRLKELGVTQLVVCGVATSAGVESTARSGHEHGYNVALATDAMTDMSPEAHDN
                     SLQRIFPR"
     gene            complement(587..1165)
                     /locus_tag="VN778_00660"
     CDS             complement(587..1165)
                     /locus_tag="VN778_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005264599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HXR49526.1"
                     /translation="MGKLIYLMTTSLDGFVADESGNFDWAMPSEEVHMFVNDTIRNVG
                     TILMGRNLYETMKVWDDIPTEGTGGPMDGPSEAMNDYAKIWRAAKKIVYSTTMTDVTI
                     ANTTIERSFDPNAIQKLVAESGYDFDIGGPHLAAAAIKAGIVDEYHQIIAPVMIGSGN
                     YWLPKAVKSKLELVDVRKFENGFAHLQYRKQL"
     gene            1271..1636
                     /locus_tag="VN778_00665"
     CDS             1271..1636
                     /locus_tag="VN778_00665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49527.1"
                     /translation="MSEKQNLIELKDGIIYYTQAGDQTQKISTNTIEAIRLLAEGLPE
                     VKLLVDYGASGKMSKESLQSGLYAMQSLRLDKVAIIWASPYLIELVSSMARTAGKSKI
                     IHFAESREAAIDWLKANRL"
     gene            complement(1700..>2463)
                     /locus_tag="VN778_00670"
     CDS             complement(1700..>2463)
                     /locus_tag="VN778_00670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49528.1"
                     /translation="QGWSSVAPTADTRPGGAVNFNNDTTAPGDPHNGALQLTTDNTTT
                     AKAQYMHNTTTPLSSVTQLSYSTKQNAPVGPIADPSYQLAICATGATTSGCNPQVSPG
                     TGSSFTTLVYEPYQGGQGAIVNNTWQNWNISSGGLFWSTHTVVCSGGTLLGTPGGPAS
                     YTLAQVQSTCPDALVSQFGVNIGTNNPGYDVEADLFNFNGTTYNFEPFATATTKDQCK
                     EGGWQNVTDNNGNHFKNQGDCVSYVATNGKNPANG"
BASE COUNT          598 a          609 c          592 g          664 t
ORIGIN      
        1 ccgcgggaaa atcctctgca gactattgtc gtgagcctct gggctcatat cagtcatagc
       61 atcagttgcc aacgcaacgt tgtagccgtg ttcatggccc gaacgggcag tcgattcgac
      121 accggcgctg gtcgccacgc cgcaaaccac aagttgggtt acacccagct cttttagccg
      181 tttatcaaga tttgtattat ggaacgcgcc ccaagtacgt ttggtaattt tgatatcgcc
      241 aggctggaca ttgagttcag gcactagttc ggcccaatcg ctcggacggt cgccgccacg
      301 cgctggactc tccgtgcgtc ccggcgcgcc accagtgacg ttaaccagta cgacaggcag
      361 ccggtgccgc ctaaaggctt cggctaattt ggcgctgtta ctgatgactt gctgcataga
      421 atgagccgtc gacagggcgg taatcccttt ttgcaagtcg ataataacca gcgccgtttt
      481 ctgatctaac gcagttatcg gcatattgct ccttcttatc cataattata caccggctag
      541 catccatctg acttgtcagg gggccattag ccaaaaacga aattgcttac agctgtttgc
      601 gatactgaag atgcgcaaag ccgttttcaa actttcgcac gtccaccaac tcaagcttgc
      661 ttttaacggc tttcggtagc cagtaattgc cactacctat catgacggga gctatgattt
      721 gatggtattc gtcgacaata ccggccttta tggctgcagc cgccaaatgc gggccgccaa
      781 tgtcgaaatc ataacctgac tcggccacta atttttgtat ggcgttaggg tcaaaagacc
      841 gttcaatggt cgtattagct attgtgacat cagtcatcgt agtggaatag actatcttct
      901 tggctgcccg ccaaatttta gcgtagtcat tcatagcttc acttggcccg tccataggtc
      961 cgcctgttcc ctctgtagga atgtcatccc agaccttcat agtctcgtac aagttgcgtc
     1021 ccattagaat cgtccctaca ttgcggatag tgtcattgac aaacatatgc acttcttcac
     1081 ttggcatagc ccagtcaaag ttgccagact catcagccac aaagccatcg agtgatgtcg
     1141 tcataaggta tattaatttg cccataaacc tattgtctca tagggcacgg cagagcttgc
     1201 tacaataaac ctatgaaaac ccgccactta aatcatgtaa gtatggcgga ataccaggtg
     1261 ctatcgtagt atgagcgaga agcagaatct tatagaactt aaggacggta taatttacta
     1321 tacccaggcc ggtgatcaaa cccagaaaat ctccacgaac accatcgagg ctataaggct
     1381 gctggctgag ggtttacctg aagtcaagct tttggttgac tatggcgctt cgggcaagat
     1441 gagcaaagag tctttacaaa gcggcctcta cgcaatgcag agtttacgcc tcgataaggt
     1501 ggctatcatt tgggcctcgc cctatcttat agaattagtg agcagcatgg cccgtactgc
     1561 cggcaaaagc aagataattc atttcgccga aagccgtgaa gccgcgattg actggttaaa
     1621 agccaacagg ctatagctgc atcactaagt acgcgttaga acgcccgaaa ctgtatgtat
     1681 atgtgcttta ttaactttat taaccgttag ctggattttt gccatttgtg gcaacgtagc
     1741 ttacgcagtc gccttggttc ttaaagtgat tgccgttatt gtcggttaca ttttgccaac
     1801 cgccttcttt acactgatcc ttagtagtag ctgttgcgaa gggttcgaaa ttgtaagttg
     1861 taccgttgaa gttaaacaga tcagcctcga cgtcgtagcc aggattattt gttccaatat
     1921 ttacgccgaa ttgggagacc agcgcatcag gacatgtgga ctgaacttgt gccagtgtat
     1981 aactggcggg tccgcccgga gtgcctaata aggtgccccc agaacacacc acggtgtggg
     2041 ttgaccagaa tagtccgccg gagctaatgt tccagttctg ccatgtatta tttacaattg
     2101 cgccctggcc gccttgatat ggctcatata ctaaagtcgt aaagctggac cctgtccccg
     2161 gggaaacctg aggattacat ccgctcgtcg ttgctccggt tgcacagatg gctaattgat
     2221 atgaagggtc ggcaataggt ccaacaggag cattttgctt tgtggaatag ctaagttggg
     2281 tcacagagga aagcggagta gtcgtattat gcatatactg cgctttggct gtcgttgtat
     2341 tatccgttgt cagttgtaat gccccgttat gcggatcacc tggagctgtt gtatcgttat
     2401 taaaatttac cgctccacct ggcctcgtat cggctgttgg ggctaccgag gaccagcctt
     2461 gtt
//
LOCUS       DATNGP010000027         3801 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_3504520, whole genome shotgun sequence.
ACCESSION   DATNGP010000027 DATNGP010000000
VERSION     DATNGP010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3801
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3504520"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /locus_tag="VN778_00675"
     CDS             <1..230
                     /locus_tag="VN778_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007916898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXR49529.1"
                     /translation="AKEVSFSRQAVAKHITVLKKVGLIRERRAGKEIQFSIEPSGLTA
                     AIQELSAAAELWDARLQKIKQIAEAIHQDNC"
     gene            complement(466..588)
                     /locus_tag="VN778_00680"
     CDS             complement(466..588)
                     /locus_tag="VN778_00680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49530.1"
                     /translation="MKKQQDNFRTHIPEPRWQQIVSVIVALFFIALWLWLAFRH"
     gene            complement(585..923)
                     /locus_tag="VN778_00685"
     CDS             complement(585..923)
                     /locus_tag="VN778_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019294313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HXR49531.1"
                     /translation="MNDQILVVTMNDIPGYEVVEVYGEVFGVLTRSRNIVSGFGASLK
                     SIIGGEIGAYTKLLSDSRIEATERMKQAAAAKGANAVIAMRFDTGDIGGTMNEVAAYG
                     TAVRIQPKRA"
     gene            complement(996..1667)
                     /locus_tag="VN778_00690"
     CDS             complement(996..1667)
                     /locus_tag="VN778_00690"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HXR49532.1"
                     /translation="MNMLDRLIGVVAPHECLGCGAEGGLICPGCVLLFPDLPERCYRC
                     HQASGGSLTCSSCRQTSGLRHVRAATDYEGIARDLIWKVKFAGARSAVDQMAQCMQPL
                     LAGLVTPDTLIVPAPTATSRARQRGYDQAKLLARQLAYRFGLPYVDCLARHGQSHQVG
                     ASREQRLCQLQQAYRVAKRRSVQNAHIFLIDDVLTTGATLEAAAHTLKMAGARYLDAA
                     VFAQA"
     gene            1838..2320
                     /locus_tag="VN778_00695"
     CDS             1838..2320
                     /locus_tag="VN778_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010164487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HXR49533.1"
                     /translation="MARRNRDDDDLLLEEDELTAAFLGDEAPMPADTPGQATPAPVAE
                     DEWDEEEAVPGQLAVDVYETKEKLVVKARTAGVNKNDLDVSISDNQLTIRGTLSSGAE
                     EGVENYFLQECYWGEFSRSIALPVPVKEDEIEAVLKDGVLTISFGKVQQDTVKKIQVL
                     "
     gene            complement(2389..2823)
                     /locus_tag="VN778_00700"
     CDS             complement(2389..2823)
                     /locus_tag="VN778_00700"
                     /inference="COORDINATES: protein motif:HMM:NF039493.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HXR49534.1"
                     /translation="MLKKIKGYLFVAATLLTISVPAVVPTIAASAACSHIGNQVSSGA
                     ATAAGASGTDLCGGTGVQGTDLASLAAKIVDIFSIVVGAVAIIMIIYGGFRYITSGGD
                     SNRVGSAKNTLIYAIVGLIIVALAQIIVHFVLNTASNSVSNQ"
     gene            complement(2889..3191)
                     /locus_tag="VN778_00705"
     CDS             complement(2889..3191)
                     /locus_tag="VN778_00705"
                     /inference="COORDINATES: protein motif:HMM:NF016820.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbC/VirB2 family protein"
                     /protein_id="HXR49535.1"
                     /translation="MIYTLADLHSDAHQGLDQVGGGGINIESVFTTIVDILSIVVGVI
                     AIIVIIYSGLKYITSGGDANKVGSAKNTLIYALIGLAIAALAQFLVHFVLFQTTKT"
     gene            complement(3209..3607)
                     /locus_tag="VN778_00710"
     CDS             complement(3209..3607)
                     /locus_tag="VN778_00710"
                     /inference="COORDINATES: protein motif:HMM:NF039493.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49536.1"
                     /translation="MKRLITTLAIAGALLFPALALTVPAGAAIDIFKHSCSGAASSSD
                     VCQSVSEQQKSGSNPVINVLKAAIETIAYITGALAIIFIIISGLRFITAAGSPEGVAS
                     ARTSLIYALVGVAVSALAGSLVAFVLDNMH"
     gene            complement(3604..>3801)
                     /locus_tag="VN778_00715"
     CDS             complement(3604..>3801)
                     /locus_tag="VN778_00715"
                     /inference="COORDINATES: protein motif:HMM:NF039493.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49537.1"
                     /translation="ILTFVFAILGALSVLMIVIAGLRFVTGSSSPQEIAKARNTIVYA
                     LIGLLIALAAESIVRLAWSHL"
BASE COUNT          980 a          968 c          983 g          870 t
ORIGIN      
        1 tagccaagga ggtgtccttc agccgacaag ccgttgccaa acatataacc gttttgaaaa
       61 aggttgggtt aattcgggaa cggcgtgccg gcaaagaaat tcagttttcg atcgagccaa
      121 gtgggctgac tgcagctatc caggagttat ctgcagcagc agagctgtgg gacgcaagat
      181 tgcaaaaaat taaacagatc gccgaagcca tacatcaaga caactgttag cacaatgtcc
      241 aaacccctat ccagccagat acttttctta tactgcagga gccgttgata cctctgctca
      301 atctccccgt atgagggtca tgtcaaagtc gtacgaaaac atatgttttg gtatgactct
      361 tgtttgactc tggcataggt ggttgtccgg cactctcaaa gaaggggtac tgagcttaaa
      421 gtgaagccct gaccaatcga cgggcagtac gatctcgcgc ttcacttagt ggcgaaaggc
      481 gagccacagc cacaacgcga taaaaaagag cgcgacgata acactgacta tctgctgcca
      541 gcgaggctcg gggatatggg tgcggaagtt gtcttgctgc tttttcatgc tcgtttgggt
      601 tggatgcgaa cggcggtacc gtaggcggcg acctcgttca tggtgccgcc aatgtcgccg
      661 gtgtcgaagc gcatagcgat gacggcgttg gcgcccttcg cggcagcggc ttgtttcatc
      721 cgctccgttg cttcaatccg gctgtcgctt aaaagtttag tgtaggcacc gatttcgcca
      781 ccaataattg acttcaggct ggcaccaaaa ccgctgacga tgtttcggct cctcgtcagc
      841 acgccgaaaa cttcaccgta gacctctacg acttcgtagc cgggtatatc gttcatggtt
      901 actactaaaa tttggtcatt catagcgtgt ccttgcgtta atagtcacgt taatagtcat
      961 tagtatgata catcaaactt acaagcctca gagtattacg cttgggcaaa tacggcggca
     1021 tcgaggtagc gggcaccggc catttttaac gtgtgtgcgg cagcttccag ggtagctccg
     1081 gtggtgagca catcgtcgat caagaaaatg tgcgcatttt gtacggagcg acgcttagct
     1141 acacgatagg cctgctgcaa ctggcataga cgttgctcgc gagaagcacc aacttgatgc
     1201 gactggccat gacgcgctaa gcagtcgaca tagggcagac cgaatcgata cgcgagctgg
     1261 cgagcaagca gtttggcttg gtcgtaacca cgctgacggg cgcggctcgt ggcagtaggt
     1321 gccggcacaa tgagcgtatc aggcgtgacg agcccggcaa gcaacggctg catgcactgt
     1381 gccatctgat cgacagccga gcgtgctcca gcaaatttca ctttccagat caggtctcgc
     1441 gcgatccctt cgtaatccgt ggccgccctg acatgtcgca gcccgctagt ttgccggcag
     1501 cttgagcacg ttaggctgcc cccagaagct tggtgacagc gataacagcg ttccggcaga
     1561 tcaggaaaga gcagcacgca gccagggcag atcagcccac cttcagcacc acagcccagg
     1621 cattcatgtg gcgccaccac gccaataagt cgatcaagca tgttcatgtt gtatttatta
     1681 tgttgtaaat aagtgctcta agcagttgca cacctttcat gacctaagct atactgctac
     1741 taacacaaaa aggcaaggct ttttaaacta tttttaatat ttctggattc cccgtactcg
     1801 aaagggaata acagcatacc gtggagggta ataacttatg gcgcgacgta atcgcgatga
     1861 tgatgattta ctactagaag aagatgagct gaccgcggct ttcctcggcg atgaagcacc
     1921 catgcccgcc gacaccccgg gccaggcaac ccctgcaccg gtagctgaag acgagtggga
     1981 cgaagaagaa gccgtgccgg gccagttggc cgtcgatgtc tacgagacaa aggagaagct
     2041 ggtcgttaaa gcccgcacgg ccggcgtcaa caagaatgac ctcgatgtca gcatctccga
     2101 caatcagtta acaatccgcg gcacgctcag tagcggtgcc gaggaaggtg ttgagaacta
     2161 tttcctgcaa gagtgctact ggggtgaatt ctcccgcagc attgccttgc cggtaccagt
     2221 taaggaagat gagattgaag ccgtcctcaa ggatggcgtc ctgaccatca gcttcggcaa
     2281 agtccagcaa gacacagtca agaagatcca agtactataa gcctaccggg cttagagcca
     2341 attaaaaacc ctgcgaatta tgcagggttt ttaatttgag cttatttgct actgattgct
     2401 aacggagttg ctagccgtgt tgagtacgaa gtgtacgatg atctgcgcta acgctacgat
     2461 gatcaagccg acaatagcgt agatcagggt atttttggcg gaaccaaccc tgttactatc
     2521 accacccgaa gtgatgtagc ggaaaccgcc gtagatgatc atgatgatgg ccacggcccc
     2581 cacgacaatc gagaaaatat cgacgatttt tgctgccaaa gaggctagat cagtaccttg
     2641 tacacctgta cctccacaga gatctgtgcc gctagctccg gctgcggtag cagcccccga
     2701 agaaacttga ttcccgatat gcgagcaagc agccgaggcg gctatggttg gcacgactgc
     2761 cggaacactg attgtaagta gcgtagctgc tacaaataag taacctttta ttttcttaag
     2821 catttcttac ctttctttat atcttcttta acttatagca caaccgatac cacttggcaa
     2881 cgctcgtttt acgtcttggt agtttggaaa agcacgaagt gaaccaggaa ttgggccagg
     2941 gctgcaattg ccagaccgat caaggcatag attagggtat ttttggcgga accgactttg
     3001 tttgcgtcgc cccctgaagt aatgtattta agcccgctgt agatgatgac gatgatagca
     3061 ataacaccaa caacaatact taagatgtcg actatagtcg taaaaacaga ttcaatgttt
     3121 atcccgccgc cgccgacctg atcgagcccc tgatgggcat cgctgtgtaa atctgccagt
     3181 gtatatatca ttgcttgttc ctcataaatt aatgcatatt gtccaaaaca aacgctacga
     3241 gagaccctgc cagcgccgaa actgcgactc ctaccagggc ataaatgagc gaggtgcgtg
     3301 ctgaggccac cccttcagga ctgccagcgg cagtaataaa tcgtaagcca ctgataataa
     3361 taaagatgat ggcgagagcg cccgtaatat acgcaatagt ctcgatagcg gctttgagta
     3421 cattgattac cggattgctg ccacttttct gctgttcaga aacgctttga cagacatctg
     3481 agctgctagc tgccccgctg cagctatgct tgaaaatgtc tatggccgcc cccgctggga
     3541 cagtcaatgc caaagccggg aagagcagcg cgccggcaat ggccagcgta gtgatcaaac
     3601 gtttcataga tgactccagg ctagccggac gatcgactct gccgcgagtg caatcagtag
     3661 accgatgagc gcatacacga tggtattccg ggctttggct atctcttgtg gactgctgct
     3721 gccagtgacg aagcgcaagc cggcgataac gatcattagc accgaaaggg ctccgaggat
     3781 agcaaaaaca aatgtcagta t
//
LOCUS       DATNGP010000028         2495 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_1765022, whole genome shotgun sequence.
ACCESSION   DATNGP010000028 DATNGP010000000
VERSION     DATNGP010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1765022"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..338
                     /locus_tag="VN778_00720"
     CDS             <1..338
                     /locus_tag="VN778_00720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49538.1"
                     /translation="TLPNGKTYIFYHVLTSPRDHQSQDRLLYRSEYNANSQNKLYVPD
                     GVSKPGATSDDKRVYYYDKRTGRYIPAAQRVSQRTQSGGASSATGTQKSEKQYLQISV
                     PTIGHDGRP"
     gene            775..1899
                     /locus_tag="VN778_00725"
     CDS             775..1899
                     /locus_tag="VN778_00725"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HXR49539.1"
                     /translation="MAIILGLCTYWARSKQVLLLVLLPFAWALAELVRSYSLALFFIG
                     PGSSLSPNWNFGALGLAMTATPFAFLGRFVGLYGLSAVAVVINIAIYLTIIKRFKLAV
                     SVMGVVLVLNFVAWRLYAPSSNHYLHVVAVYLHNDGGSLNPWGSIDLPPKDTDVLALP
                     EYSHFFENPHYQEFAEANFADNTAIVTTQVSKGASPVSNNLTYYSNRTGFVSVQSKTF
                     LAPYGEYLPYSATELLKAVGQQAALTRFRHDDQVRKGKKPEQVVTVNGIKIGGLACSG
                     VLNLNEYRRLAHEGADVLTNSASLSLLETAGLYRVQEMYQNRFHAIANAKPFVQSARS
                     GEAYIISSDGHVIASASDKSRLLEATVPVQSKRTVYSWLP"
     gene            complement(1896..>2495)
                     /locus_tag="VN778_00730"
     CDS             complement(1896..>2495)
                     /locus_tag="VN778_00730"
                     /inference="COORDINATES: protein motif:HMM:NF039195.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PsbP-related protein"
                     /protein_id="HXR49540.1"
                     /translation="MGSKRRPLIFLGLLLLVVVVGILVYEHGKNTNQNAPQASSTAAS
                     KPSVSVPPSWKQYTLKYEKLSFSYPDSWQLQDTSKSGQDTVTLTGTNGYTASLQSGND
                     ARPSIDANPKILLAESEKLAGQAVYLDFLADQTGNVSQVYVSTSKSDASAAPSARHAG
                     GLLIARSDYPASSPSESLLATTHDENYLNTMTVLNTMHY"
BASE COUNT          581 a          633 c          637 g          644 t
ORIGIN      
        1 tgacccttcc caatggcaaa acctacattt tttaccatgt tctgacctcg ccccgggatc
       61 atcagtcgca agatcgatta ctgtaccgtt ccgagtataa tgcgaatagc caaaacaagc
      121 tatacgtgcc ggatggtgtg agcaagccgg gagctactag tgatgataag cgcgtttact
      181 attatgacaa acgtaccggc cggtacattc cggcagccca acgtgtctcg cagcggaccc
      241 agtcgggtgg ggcgagcagc gcgaccggca cacagaaatc agagaagcag tatcttcaga
      301 tttccgtgcc caccatcggc cacgatggcc ggccataata ggtccgcctg acgagtaagc
      361 gcaagccggc tgcgcagcag ccacgcattg taaagccaaa aaccactctt tataatagga
      421 ttcgaaaccc ctgagagtgg agactacatc aaaattacga ctatattatt taactggcgc
      481 ttgctgcccg cggtaaccgg gcttatatcc ggactagcct ttctcaacaa tagtttaggc
      541 gcgttggttc tggttgggct tcttccgttt gtttggtaca cgcgccaata tacaagttta
      601 tccctgcggc aaatcttctt cgactgctgg cgcggggcat tcgtttttac acttatcgca
      661 ggggcctgga ccctgcagac tgaaccatct aactggcttg gcataatcgg aactacggct
      721 accttcgtca aaatcactac ctggatactc atcgcggcgt ggtcaagtct ttctatggct
      781 ataatcctag gcctttgcac ttactgggcg cggagtaagc aggtactctt actggtcttg
      841 ctaccatttg cttgggcttt ggccgagtta gtgcgcagtt atagcctggc cttgtttttt
      901 attggccccg gaagcagctt gagccctaac tggaattttg gcgcgctcgg tctggcaatg
      961 acagcaactc ctttcgcctt tttaggacgc tttgtcggtc tgtatgggct gagtgctgtc
     1021 gcagtcgtga ttaatattgc gatttatcta accatcatca agcgctttaa gcttgccgtg
     1081 agcgttatgg gggtagtttt ggtactgaat tttgtggcct ggcgactgta tgctcccagc
     1141 tctaaccact accttcacgt agtagcggtc taccttcaca atgatggcgg ctcactgaat
     1201 ccgtggggaa gcattgacct cccccctaaa gataccgatg ttctggcctt gccggagtat
     1261 tcacactttt ttgaaaatcc tcactatcag gaattcgcgg aagctaactt tgccgacaac
     1321 actgcgattg taaccacgca agtcagcaag ggagcgagtc ctgtatctaa taacctcacc
     1381 tattattcaa accgtactgg ctttgtcagc gttcagtcta aaactttttt ggctccctac
     1441 ggagagtatc tgccctatag cgcaacagaa ttattaaaag cagttggaca gcaggctgct
     1501 cttactagat ttcgacacga cgatcaagtt cgaaagggca aaaaacccga gcaagttgtg
     1561 acggttaacg gtataaagat tggcggcttg gcctgttcag gcgtgcttaa ccttaatgaa
     1621 taccggcggc tcgcccacga gggcgccgac gtcttaacta acagcgcctc cctcagcctt
     1681 ctagaaacag ccggtctcta ccgagtacaa gaaatgtatc agaaccgttt tcacgctatt
     1741 gccaacgcca agccctttgt gcaatcagcc cggagcggcg aagcgtacat aatatcgagc
     1801 gacggccacg taattgcctc cgctagcgac aaatcgcgac ttctggaagc tactgtacca
     1861 gtgcagtcta aaaggacagt gtacagctgg ctgccttaat aatgcatggt gtttagcacg
     1921 gtcatggtgt tgagataatt ctcatcgtgc gttgtagcaa ggagtgattc gctcggactg
     1981 ctggcgggat agtcggagcg agcgataagt agtccgccag cgtgccgagc ggaaggtgcg
     2041 gctgaagcat cgcttttgct agtagaaaca tacacttggc tgacgttgcc ggtctggtcg
     2101 gcgaggaagt ctaggtaaac tgcttgtccg gccaactttt cagactcggc cagcagtatt
     2161 tttgggttgg catctataga agggcgggcg tcgttgccgc tttgcaggct ggcggtgtaa
     2221 ccgttagtgc ctgtcagggt gacggtgtcc tggccgctct ttgaagtatc ttgtaattgc
     2281 cagctgtccg ggtaactaaa gctcaatttt tcgtatttca aggtgtattg cttccagctc
     2341 ggtggaacgc taacacttgg cttcgaagct gcagtagaag aggcttgggg agcattctga
     2401 ttggtatttt taccgtgctc gtaaaccagg atgccaacca cgacgaccaa taacaatagc
     2461 ccgagaaaaa tgagcggcct tcttttacta cccat
//
LOCUS       DATNGP010000029         2547 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_2352905, whole genome shotgun sequence.
ACCESSION   DATNGP010000029 DATNGP010000000
VERSION     DATNGP010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2547
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2352905"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1501
                     /locus_tag="VN778_00735"
     CDS             <1..1501
                     /locus_tag="VN778_00735"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HXR49541.1"
                     /translation="MEAMDLMDPRRRLGHNIRLMIGYVLVGIALLLLTIILLFVAYGF
                     GLQNGKVIQSGLLFLSSTPNPAHVYLNGVQYQNDTNTKLLLQEATYRVTLTRAGYRDW
                     ERPISVVGGQVEFYRYPFLFPLDLTAIAAQTYQSAPTLVSESNDQNWLLVSQAGKLDS
                     FDVYNLANPGQKPTVITLPAGTLTTAAGVQSLAAVAWANDDNHLLLKHTYGPNTEYLL
                     LNRSSPASSQNITKILQLPPTGITLSLNNGQSDHYLVYDATSQVLSQNSLGSPSLVPL
                     VDHILAFAADNDSSDTIAYVTPDSTNPLQVSVMIYNGGKKYLIRHEAAGQHYLLAATD
                     YSGDTYVAISAEEANTAYVYKDPVEQITDPRFGVAVPAQAFTVTHPTYAGFSISGQYA
                     VFENGARFAVYDVDNDQGFSYNVTDSLDSPMTHAEWMDGARLYYISHSQVVVFDYDGR
                     NRQVLTPADSRYPLFFDSNYKFMYAFTTDPANKLQEILTTTSLRTKADQ"
     gene            complement(2047..>2547)
                     /locus_tag="VN778_00740"
     CDS             complement(2047..>2547)
                     /locus_tag="VN778_00740"
                     /inference="COORDINATES: protein motif:HMM:TIGR01076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class E sortase"
                     /protein_id="HXR49542.1"
                     /translation="DYTQTSDDENAIQNALEKGIVHYPSTVFPGQQGNAAYFGHSSNN
                     IFNPGKYKFAFVLLHELQPGDTFYLTYQGKSYVYKVFKRIVVEPDQVDVLNNVPGHPA
                     TATLITCDPPGTALHRLVVVGDQISPDPLQNASGSNTTSSNPTVTALPDNGPTLWDRI
                     TSFLNL"
BASE COUNT          594 a          685 c          656 g          612 t
ORIGIN      
        1 catggaagca atggacttga tggatcctcg gcgtcggctc ggacacaata tccgtttgat
       61 gatcggctac gtcctggttg gtatcgccct gctgctcttg accatcatcc tactattcgt
      121 cgcttatggt ttcgggctgc aaaacggcaa agttatccag agcgggctac tgttcctgtc
      181 cagcacgccc aacccagccc acgtctacct gaatggtgtg cagtaccaga atgacaccaa
      241 tacgaagcta ctgctccaag aagcaacata ccgcgtaaca ctcacgcgtg ctggctaccg
      301 cgactgggag cggccgatca gcgtggttgg gggccaggtt gaattttaca ggtatccgtt
      361 cctgtttccg cttgacctta cggctattgc tgcccagacc tatcagtcgg cgccaacctt
      421 agttagtgaa agtaacgacc aaaactggct gctcgtatcc caagccggca agcttgactc
      481 gtttgacgta tacaacctcg ctaatcccgg tcaaaagcca accgttatta cattgccagc
      541 gggcaccttg accacggcag ctggcgtgca gagcctggcg gctgtcgctt gggcgaatga
      601 cgataatcac ctgctgctca agcataccta tggaccaaac accgaatatc tgcttttgaa
      661 ccggtcgtcg ccggcgagct cgcagaacat taccaaaata ctccaactgc cgccgactgg
      721 cattacactg agtttgaata acggccagag tgaccactac ctggtgtacg acgctacatc
      781 acaggtgttg tcgcaaaaca gcctcggttc cccgtcgctg gtaccgctcg tcgatcatat
      841 ccttgccttc gcggctgaca atgatagtag tgacacgatt gcttacgtga cccctgatag
      901 tacaaatccg cttcaggtca gcgtcatgat ttataatggc ggcaaaaaat atctcattcg
      961 tcacgaagcg gctggccagc attacctgct tgccgccacc gactacagcg gtgatacgta
     1021 tgtagcgatc agtgccgaag aggcgaacac ggcctatgtt tacaaagacc ctgtggaaca
     1081 aattaccgat ccgcggtttg gggtggcggt gccggcccag gccttcaccg ttacccatcc
     1141 tacctatgcg ggcttctcga ttagcggaca gtatgcggtg ttcgaaaatg gcgcgcgttt
     1201 tgccgtctat gatgtcgata acgaccaagg gtttagctat aatgtgaccg attcgctcga
     1261 ttcgcctatg acccacgccg agtggatgga cggcgcccgg ctttactaca tcagtcatag
     1321 ccaggtagta gtgttcgatt acgatggccg caaccgccaa gtgttgaccc cggccgactc
     1381 acgctaccca ctattcttcg acagtaacta caaattcatg tacgccttca caacggatcc
     1441 agccaataaa ttacaggaga tcctgacgac tacctcgctg cgtaccaaag ctgaccagta
     1501 acatctttgt tcctttccaa ggaactcaac cccatacttt tggcattgcc caaaagtatg
     1561 caaaaggcta ggcccggcgt cgtctgtact tgggtgccgt tgtaaagaat cacaagcgtc
     1621 tgcggaacgt ctcgcaaaaa ccttgctctt cgagcaatgg tttttgctcg ttgaacggcc
     1681 ctcggctggg tcttcttgtg atttttacga acagcacgcc acgcgcccga cgccaatggg
     1741 ccagaaaacc gccgtggagc ggttcctgcc gctattgatc actagcggcc ttcggccgcg
     1801 ccatggcttg ctggcccttt ggagcgaacc cggcggacaa gttgtataaa gttacaactc
     1861 ttcgcctaga aggcgaacgc ggagtactct agcgaagcga atgtacggag tggttgtata
     1921 cttttacaaa ttgaacccaa gtgagtgaaa cggcctaggc tttttgctta ctttttgcca
     1981 atgaaaaaag taagagagat tggttctttg gaaagaccaa gattggccct tttgataaag
     2041 attaatttaa agattcaaga atgacgttat tcggtcccag agcgtcgggc cgttgtccgg
     2101 cagtgcggtt acggtcggat tgcttgaggt cgtattgctg ccggaagcat tctgtaaggg
     2161 gtcgggactg atctggtcgc cgactaccac cagccgatgc agggcggtac ccggcgggtc
     2221 acaagtgatg agcgtggctg tcgccgggtg gcctggaaca ttattgagta cgtctacctg
     2281 gtccggctcg accactatcc gcttgaacac cttgtagacg tacgatttgc cttgatatgt
     2341 caaataaaag gtgtcgccgg gctgcagttc gtgcagcagg acaaaggcga acttgtactt
     2401 accggggtta aaaatattat tactggaatg gccaaaataa gcggcattac cctgttgacc
     2461 gggaaatacc gtcgacggat agtgtacgat tcccttctcg agggcgtttt ggatagcgtt
     2521 ttcgtcatct gacgtctgcg tataatc
//
LOCUS       DATNGP010000030         7885 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_2883063, whole genome shotgun sequence.
ACCESSION   DATNGP010000030 DATNGP010000000
VERSION     DATNGP010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 7885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7885
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2883063"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1100
                     /locus_tag="VN778_00745"
     CDS             <1..1100
                     /locus_tag="VN778_00745"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49543.1"
                     /translation="ITDLQVQSASSKLAWPGVGQSAVGIVGTGILETHGTQTPAPTAS
                     TAKIITVLNVLRIKPLQPGQQGPTITLTANDAAIYNAYVAEGGSVVPVQAGEQISEYQ
                     MLQAILLPSANNMADSLAIWAFGSLPAYAAAATSYLHQLGLSETHIGADASGFSPTTT
                     STAHDLVRLGELAMQNPVLAGIVAQPSASGIPLTTGVKNVNYLLGTNNIIGVKTGNTD
                     QAGGVYVSASRAVVNGKLVTLVTALVGAPTLFESMKASVPLIRSAQANFATTSVITAG
                     AVVGRYHTPWSGSVGAIASKDLNVTTWNDSTVSASINLQPVSAKARADKTVGTVSSSG
                     SSPFTSQQPISVKLQNAPIAPSILWRLTHPF"
     gene            complement(1675..1751)
                     /locus_tag="VN778_00750"
     tRNA            complement(1675..1751)
                     /locus_tag="VN778_00750"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1715..1717),aa:Val,seq:tac)
     gene            complement(1766..1852)
                     /locus_tag="VN778_00755"
     tRNA            complement(1766..1852)
                     /locus_tag="VN778_00755"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1816..1818),aa:Leu,seq:gag)
     gene            complement(1915..3048)
                     /locus_tag="VN778_00760"
     CDS             complement(1915..3048)
                     /locus_tag="VN778_00760"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HXR49544.1"
                     /translation="MVLAILSSAIGISYATANRGLLSARQAQENSQMTENVQTQIEFL
                     RTLAPLGTNDSTSSAYNAADNIYYPSGAYCLKNLPATPTIETNASNCWFPAGAFQYQL
                     LIYNCNNMPADGHCINTILTTDTFVVEATWPDVTGNGTDTATQAYRVHPPTGIAALSG
                     GGGGSGGGGVCTTGSAHDIVMAFDASGSMGEAGYDGLTRGQNMQTAATIFANSVGLSS
                     SGNHVSIIDFYKVFMVDTLLDTPSSPFSSDQSAAVNAIANKYKPMPVPGQPTTDFNFA
                     LLQAKSYLTGGSSRPSPVHKVLVFYSDGGQDVPDTPDADTITEGIADQLKAAGIEIFS
                     INAWQQAAIMENIATPGDYRWAPDTKTLNKAFSDLASTLICTP"
     gene            complement(3213..4850)
                     /locus_tag="VN778_00765"
     CDS             complement(3213..4850)
                     /locus_tag="VN778_00765"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HXR49545.1"
                     /translation="MSKVAHYLQEHLVGEVMTSTDARRYFATDASIFQVAPALVVYPR
                     NENDVRKTARFTWQLAERGRVIPMTARGSGTDQTGAALGGGIMLVFPAHLNRILELDT
                     KNNTVTVEPGIGFGKLQQALHTHGRFVPPAPASSEYSTIGGAIANNASGENSVKYGDM
                     RSYVQSLRVVLANGEVIETGRLGKRDLSKKLGLATFEGEIYRSVDTLLEEQRELIARL
                     QINVTKNNAGYDILDIKRRDGTFDLTPLIIGSQGTLGIVTEATIATEAHNPQTTLMLA
                     SFDSLAGLQDAVNELRNLPELPSAMELIDGNVLEQVHALNPNQLKDIMAPPFPHAVLL
                     AEFDAGNDRQQKKLIKKAHKIFEKHAISLQTAAEPEQQQLFWKLRQATGTLISHNDGL
                     KRATPVINDAAVPPDRVRELMEGIYRLSEASNLPLAVWGHAGEGSVHVQPKLNLAQVG
                     DRQRAFRLIEEYHKLVLSLGGTLSSESGDGRLRAPYLEAQYGPELYAILQKVKQIFDP
                     YGTLNPGVKFGTSIDDLKAIIRSDYGLEHLYDHLPRS"
     gene            complement(4939..5026)
                     /locus_tag="VN778_00770"
     tRNA            complement(4939..5026)
                     /locus_tag="VN778_00770"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4990..4992),aa:Leu,seq:cag)
     gene            complement(5116..6897)
                     /locus_tag="VN778_00775"
     CDS             complement(5116..6897)
                     /locus_tag="VN778_00775"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HXR49546.1"
                     /translation="MRFRLIRLRFRRQLRASQRQVEGLGTQAEQQLEEHFFKRFSRLN
                     TVRRFVIGWVLLVILLIGGVVLQNIWLSNSYQALKPVAGGIYNEGVLGTFTNANPLYA
                     TSDADTSVARLIFAGLFTYDQQNKLVGDLASGYQVDARGTTYTVRLKRGLTWQDGQPL
                     TSADVRFTYQMIQNPDAQSPLQASWQGITITAPDARTIVFKLPDPLASFPYNLTNGIV
                     PQHLLARIPPASLRSADFNTVHPVGAGPFQWQAIQVAGSDPSTAEEQIALTPFSNYQG
                     GEPKLHEFIMHVFASRDQLVSAFKAGRLTGAEGLNSLPSDIAGMSSLQAHNLLLRAAT
                     MVFFKTSDGVLADKQIRLALVQATNTKRIITNLGYPTLAVREPLLIGQLGYDSTLAQS
                     GLNLTAARAALDSDGWTVGKDGIRTKGGQPLAFTLTAADTPEYRQVTHELQQEWRAIG
                     VQLQVQLLDAADFQSTLTYHNYDAVLYGISIGVDPDVFVYWDSSQADIRSSNRLNLSE
                     WKNATADAALEAGRTRLNPTLRVIKYKPFLQVWQQDAPALGLYQPRLLYLTNGPVAGL
                     TSSSINTAPDRFNNVQNWEIREAKVTD"
     gene            complement(6907..7122)
                     /locus_tag="VN778_00780"
     CDS             complement(6907..7122)
                     /locus_tag="VN778_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010164235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HXR49547.1"
                     /translation="MSIFNYLTIGSMLLMTLLILVQTRGASLGAGLGGAGEVNTTRRG
                     TDKTIFQLTIVCALVFSLSLIFGVIVK"
     gene            complement(7174..7551)
                     /locus_tag="VN778_00785"
     CDS             complement(7174..7551)
                     /locus_tag="VN778_00785"
                     /inference="COORDINATES: protein motif:HMM:NF015953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HXR49548.1"
                     /translation="MDKKFARKPLVRFLTRWLVSSLGLWIAAGFLSSSITLDNKLKSA
                     IVAGLILAIVNTVLKPILIIFSLPAILFTLGLFMIIINGLTVFIASKLYTPLHITNFW
                     AAVFAGMIIGLVNFLVGAILKEL"
     gene            complement(7570..>7885)
                     /locus_tag="VN778_00790"
     CDS             complement(7570..>7885)
                     /locus_tag="VN778_00790"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HXR49549.1"
                     /translation="GMIMSDYEQQVEHFMGEKLRELGLIKTITHENVRLYYPHATSHF
                     LGLNVHDVGDYERPLEAGVVMTVEPGIYIPEEGIGVRIEDDVLITSAGIKILSEKLSR
                     DL"
BASE COUNT         1926 a         2065 c         1944 g         1950 t
ORIGIN      
        1 ccattactga tttacaagtt caaagtgcct ccagcaagct ggcctggcct ggcgtcggtc
       61 aatccgccgt cggcatcgtc ggcactggca tcttggaaac tcacggcacc caaacgccgg
      121 cacctaccgc cagcaccgcc aagattatca ccgtcctgaa cgtcttgcgc atcaaaccac
      181 ttcagccggg ccagcaaggc cccaccatca ccctaactgc caacgacgct gctatctata
      241 atgcttacgt agccgaaggc ggctccgtcg taccggtgca agccggcgaa cagattagcg
      301 agtaccaaat gctgcaagct atactgttgc cttctgccaa taatatggca gatagcttgg
      361 caatctgggc ttttggctca ttgccggcgt acgccgcagc cgctactagt tacttgcatc
      421 agctcggctt aagcgaaacg catatcggtg ccgacgccag cgggttttct cctactacca
      481 ctagcacagc tcatgactta gtccggcttg gtgaattagc catgcaaaat ccggtgctcg
      541 ccggcatcgt cgcgcagccc tcagccagcg gtattcccct aacgactggc gttaaaaacg
      601 tcaattactt gctcggtacc aacaatatca tcggtgtcaa aaccggcaac accgaccagg
      661 ccggcggcgt ttatgtctca gcctcccgag ccgtggttaa tggcaaactg gtcactctcg
      721 ttaccgctct agtgggcgcc cctaccctct ttgaatccat gaaagccagc gtccccctca
      781 tccgtagtgc acaggctaat tttgcgacca cttcggtaat cactgctggc gctgttgtcg
      841 gtagatacca cacgccctgg agcggtagtg tcggtgctat agctagcaaa gatttaaacg
      901 ttactacttg gaatgacagt actgtttcag cttcgattaa tttgcagccc gtctcagcaa
      961 aagcccgtgc agataaaact gtcggtaccg tgagctcttc aggctcttct ccctttactt
     1021 cccagcaacc tatatcagtt aagctacaaa atgcccctat agcgccatca atactttggc
     1081 gcttaaccca tcctttttga aaacgcccct atataaaaac catatcaaag cagtatgaaa
     1141 acatatgttt tcgtatgcac tgatactatt tttatatagg tgagttttta aacaatgtcg
     1201 gtcgagatgg tggagctaag cggacttcca atgatgtgct ggcttctagc caagtttttg
     1261 tcagggttag caaggataag tatcgagtac ggatagagtg ctaaggacgc cttccccagt
     1321 agtcatttgg attctctatc gaagcgcgag ctacctctcc aaaggcagta gtaacatctg
     1381 tttgtgcctc agctacagac atctgaggtg tgtcagattc agctggaaca tcagcaccaa
     1441 ctggcacgcc ttctgatcgt gctcctagcg gcaccaaata aaactcttcc tctcttccaa
     1501 atactatatc tagcgcttta ttgccaactc tgcgtgcacc tatacgaact gatgtgcgta
     1561 ttgtctctaa tggtggtggt agattgtttc tcataaatcc aacgatacac ctatattcac
     1621 attttgcaat agcgtgcaat aattatagtg tattgtcgat agcaatacta attctggtgg
     1681 gcgctgaggg attcgaaccc ccgaccccct cggtgtaaac gaggtgctct agccagctga
     1741 gctaagcgcc cattatcttg ggacctggtg cggacgagag gacttgaacc tccacaggat
     1801 tgctcccact accacctcaa ggtagcgcgt ctaccaattc cgccacgtcc gcaagaagac
     1861 caaactatct tatctgtttt acgctaaaaa ctcaaggccg agaaaggttc agcgttatgg
     1921 ggtacaaatt aaagttgagg ccaaatcact aaatgcctta ttaagggttt tggtatcggg
     1981 ggcccagcgg taatcgcccg gggttgcaat gttctccatt atggctgcct gttgccacgc
     2041 attaatgcta aatatctcta ttcctgctgc tttgagttga tctgctatcc cttcagtgat
     2101 tgtgtctgca tctggcgtgt caggcacatc ttgcccccca tcagaataga aaaccaaaac
     2161 cttatgaact ggtgatggcc ggctgcttcc accggttaag taactctttg cttgaagaag
     2221 cgcaaagtta aaatctgtcg ttggttggcc gggaacgggc atcggtttat atttgttggc
     2281 aattgcgttc acggcagctg attggtctga gctaaaggga gacgagggtg tatctaaaag
     2341 tgtatctacc ataaaaacct tatagaaatc gataattgat acatggtttc cagatgacga
     2401 taagccaaca ctgttagcaa aaattgttgc tgcagtctgc atgttttgtc cacgagtaag
     2461 cccgtcatag ccagcttcac ccatgctgcc agaagcgtca aatgccatga ctatgtcgtg
     2521 agcactgccc gtcgtgcata caccaccgcc gccggagccc cctccgccac cggataaggc
     2581 agcaatgcct gtaggcggat gcactcgata ggcttgcgtt gccgtatcag tcccgttgcc
     2641 tgttacgtca ggccaggtgg cttcaactac aaaagtatcg gtagtgagga tagtatttat
     2701 gcaatgacca tcggccggca tattgttaca gttataaatt aaaagctggt attggaaggc
     2761 tccagcagga aaccagcagt tacttgcatt tgtttctatg gtaggggtgg ctggtaagtt
     2821 tttaagacag taagcaccgg atggataata tatattatcg gccgcattgt aagcagagct
     2881 cgtgctatcg ttggtgccta ggggcgcaag cgtacggagg aactctattt gagtctggac
     2941 attttcggtc atttgggagt tttcctgggc ctggcgcgcg ctcagtagcc ctcgatttgc
     3001 agtcgcataa gaaatcccga tggcagaact aagaatagcc aaaaccatca agacctcgac
     3061 taatgtgtcc cctgactgtt ttcttactgt aaacatgcca tttgctgcta caacgactgt
     3121 tttattttgt gggcaatttt cttattcacc catctaagca taaactatat gttaagatgt
     3181 ggatttaacc taacagcacg ttgttgattt gtttaactac ggggcaaatg gtcataaagg
     3241 tgctcaagcc cataatccga gcggataata gctttgagat cgtcgatcga cgtgccaaac
     3301 ttgacgcccg gattgagcgt gccgtaggga tcgaagattt gcttgacctt ttgcaagata
     3361 gcgtaaagtt cagggccgta ctgggcctca agataaggag cgcgcaaacg gccgtcacca
     3421 ctttcgctgc tcaacgtacc accaagactt aagactagtt tgtgatattc ctcaatcaac
     3481 cggaaagccc gttggcgatc tccaacttgc gccagattga gcttaggctg gacatgcaca
     3541 ctgccctctc cagcgtgacc ccagacagct agaggcaggt tactggcttc agagagccga
     3601 taaatacctt ccatcagttc acggacgcga tctggcggga cagcagcatc gttgataaca
     3661 ggtgtggccc gtttcaggcc gtcgttatga ctaatcagcg tcccagttgc ctgccgcagt
     3721 ttccaaaata attgttgctg ctcgggctca gccgccgttt gcaaacttat agcgtgtttt
     3781 tcaaaaatct tatgggcctt cttaataagt tttttctgct ggcggtcatt accggcatca
     3841 aattcggcta gtagcacggc atgcgggaaa ggcggcgcca taatatcttt gagttggtta
     3901 gggttgaggg cgtggacttg ctcgagcaca ttgccgtcga taagctccat ggcacttggt
     3961 aattccggga gattacgcag ttcgttaaca gcgtcctgca gacccgccag actatcgaaa
     4021 ctagccaaca taagtgtcgt ctgtggatta tgtgcctccg ttgcgatagt agcttcagtc
     4081 acaatgccca gcgtgccttg actgccaatg atcagtggcg tcaaatcaaa tgtaccgtca
     4141 cggcgtttga tgtccagaat atcgtagccg gcattgtttt ttgtgacgtt aatttgtaaa
     4201 cgagcaatca gctcacgctg ctcttccagc agggtatcga cggaacggta gatttcacct
     4261 tcaaaagtcg ctagccctag ttttttactg aggtcgcgtt tgcccagccg accagtttca
     4321 ataacttctc cattcgccag cactacccgc aaactttgca catagctccg catatcaccg
     4381 tacttgaccg agttttcgcc ggaagcattg ttggcaatcg caccgccgat agttgaatat
     4441 tcgcttgagg ccggcgccgg cggcacaaaa cggccgtgcg tatgcaatgc ttgctgtagt
     4501 ttaccgaaac caatacccgg ttctaccgtc acggtgttgt ttttcgtatc aagttccaga
     4561 atccggttca ggtgtgccgg aaagacgagc atgatgccgc caccgagggc cgctccagtc
     4621 tgatcagtgc cggagccgcg agctgtcata ggaatgacac gtccacgttc agcaagctgc
     4681 caggtgaagc gcgccgtctt acgcacatca ttttcattcc ggggatacac cacgagcgcc
     4741 ggcgcaacct gaaagatgct ggcatcggtg gcaaaatagc gccgagcatc ggtgctggtc
     4801 atcacttccc ctaccaaatg ctcttgcaaa taatgcgcaa ctttgctcat cagtgtaacc
     4861 taccctattt aagatctcgc ataattgtct agcagttatg atagcacatc gccggccaga
     4921 aatgcttatg gtccgtactg gtgcgcgaga gaggacttga acctccacga gcgtaatgct
     4981 cactagctcc tgaagctagc gcgtctacca attccgccac tcgcgcgcat taccccaaca
     5041 aaagctatgc ttttgccggg gaccccttgg taaagctacc ctatcttatc tgttttgggg
     5101 caaaaactca agggtttagt cggtcacttt ggcttcgcgg atttcccaat tttggacgtt
     5161 gttgaagcga tccggggccg tgttgatact agaactggtg agaccagcga ccgggccgtt
     5221 ggtcagatac agtaagcgag gctggtagag tccaagtgcc ggggcatcct gctgccacac
     5281 ctgcaaaaat ggcttgtact taatcactcg cagcgtcgga ttcagtctcg tgcgcccggc
     5341 ttcgagcgcg gcatcagccg tggcattttt ccattctgac agattgaggc gattgctgga
     5401 gcggatgtca gcttgggagc tatcccagta cacgaaaaca tcggggtcga cgccgataga
     5461 gatgccatag agcaccgcat cataattatg gtatgttaaa gtgctctgaa aatcagccgc
     5521 atcaagcagc tggacttgca gctgcacgcc aatggctcgc cattcttgtt gcagctcatg
     5581 ggtcacctgg cgatattcgg gagtgtcagc ggcggtgagt gtgaaagcca agggttgacc
     5641 gcctttggta cgaataccgt ccttgccgac cgtccaaccg tcactatcga gtgcagctcg
     5701 ggctgcagtt agattaagcc ccgactgcgc cagagtactg tcataaccga gctgcccgat
     5761 gagcagtggt tcacgcaccg ccagtgtcgg atagcccaga ttagtaataa tacgtttggt
     5821 gttcgtggcc tgcaccagtg ccaaacgtat ttgcttgtcg gcgagtactc catcggaggt
     5881 tttgaaaaag accatggttg ccgcccgtag taacagattg tgtgcttgca gcgaagacat
     5941 gccagcgatg tcactcggca agctattcaa gccttctgcg ccagtcagcc gaccggcttt
     6001 aaaggcactg accaactgat cgcgcgaggc aaacacatgc atgataaatt cgtgtagctt
     6061 cggctcacca ccttggtaat tgctgaaggg cgtgagcgct atctgctctt cggcagtgga
     6121 cgggtcgcta cccgctacct gaatcgcttg ccactgaaac ggtccggcac cgacaggatg
     6181 gactgtattg aaatctgctg agcgcaagct ggccggtgga atacgagcta acaggtgctg
     6241 cggcactata ccgttcgtca agttataagg aaacgatgcc agcggatcgg gtagtttgaa
     6301 gacaatcgtc cgcgcatccg gagcagtgat ggtgataccc tgccagctag cctgcaacgg
     6361 cgactgggcg tccggatttt gaatcatttg gtacgtgaaa cgcacgtctg cgctggtcag
     6421 cggctgaccg tcctgccagg tcagtccacg cttaagccgt accgtatatg tggtcccgcg
     6481 cgcgtcgacc tgatagccac ttgccagatc gccaactagc ttattttgct ggtcataggt
     6541 aaataagccg gcaaaaataa ggcgcgcaac gctggtatcg gcatcgctcg tggcatacag
     6601 tggattggca ttcgtgaaag taccaagcac accctcgtta taaataccgc cagcgactgg
     6661 cttcaaggct tgataactat tactcagcca aatgttctgc aaaaccacgc cgccgatcag
     6721 cagaataacg agcagtaccc agccgatgac aaaacgccgc acggtattta gtcggctaaa
     6781 ccgtttaaaa aaatgttcct cgagttgctg ttcggcttgc gtgccaaggc cctcaacttg
     6841 gcgctggcta gcacggagct ggcggcgaaa acgcagacgt atgaggcgaa accgcatggg
     6901 gcccgcctat ttaactatca cgccgaaaat caaagacagg ctgaagacga gtgcgcagac
     6961 aatcgttagc tgaaagatgg ttttgtccgt gccccgacgg gtggtgttaa cttcgcccgc
     7021 cccgcccagg ccagcgccta aggacgctcc tcgggtctgc actagaatca gcaaagtcat
     7081 cagcaacatc gaaccgatgg tcaggtaatt gaaaatgctc attgcttacc attgtcccgc
     7141 attgtatggt tcgctgtcaa agctttttgc tcctcaaagc tctttcagaa tcgccccgac
     7201 aagaaaatta accagaccaa tgatcatacc ggcaaagacg gcagcccaga aattagtgat
     7261 gtgcagcggc gtatacagtt tgctggcgat aaagaccgtc aaaccattaa taatgatcat
     7321 gaataaaccg agtgtaaaga gaatggccgg cagcgaaaag atgatcaaga taggtttaag
     7381 tacagtgttt acgattgcca gaattagtcc ggcgacaata gcacttttca acttgttgtc
     7441 gagtgtaata ctgctactca gaaacccggc tgcaatccag aggcccaggc tactcaccag
     7501 ccagcgagtc aagaagcgga caagcggctt gcgggcgaac tttttatcca taagctcaat
     7561 tgtagattac tacaggtctc gggacaactt ctcgctcaat atttttatgc cagcactggt
     7621 gataagcaca tcgtcctcaa ttcgtacacc aataccctcc tcagggatat agatgcctgg
     7681 ctcgactgtc attaccacgc ctgcctcgag tggtcgctca taatcgccga catcgtgtac
     7741 attcagacca agaaaatgtg aagtggcatg gggataatat agccggacat tctcatgtgt
     7801 aatagtttta atgagtccca gctcgcgcag cttttcgccc atgaagtgtt cgacctgttg
     7861 ctcatagtcg ctcattatca tgcct
//
LOCUS       DATNGP010000031         4879 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_4125871, whole genome shotgun sequence.
ACCESSION   DATNGP010000031 DATNGP010000000
VERSION     DATNGP010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4879
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4125871"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..449
                     /locus_tag="VN778_00795"
     CDS             <1..449
                     /locus_tag="VN778_00795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49550.1"
                     /translation="FGTEVASLPTGLERYYERYFTNRAAVAAYTAQYQTEFTSRQATV
                     AQDDAELTSLKGQITSAENALKAKETAINAQKSQLLSERNSGNVDAYNAGVPGYNSLI
                     DGYNREAQTVQSLINQYNQLVASRNAVALEEDQLVNDLSSVSPIKQ"
     gene            504..1853
                     /gene="radA"
                     /locus_tag="VN778_00800"
     CDS             504..1853
                     /gene="radA"
                     /locus_tag="VN778_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HXR49551.1"
                     /translation="MARKASTRYVCSNCGAMQSTWSGRCPQCGEWNTLEEQLSAETLA
                     RGVKSGRALVAQPVDKSIAADNQRLVTGMAEVDDVLGGGIVTGSVNLIAGQPGIGKST
                     LLLQLAYAVAANVPVLYVSGEESAHQIGMRAGRLGTLRPQLQLATSTSADDIAATIAG
                     GAYKLVIIDSIQTVTCSTITSAAGTVSQITNSTQLLTMAAKQSDTAIVLVGHVTKEGS
                     IAGPKVLEHVVDVVLQLEGDRYGGFKVLRAAKNRFGSTNEAGIFEMVEQGLQPVENPS
                     AALLAERQISDGSVVLATLEGTRPLLVEVQALVNRTSYGYPKRAASGFDLNRLNLLVA
                     MLERRTKLNLSEQDIYINIVGGIQIREPAADLAICMAIGSAAKGLQLKKNAAVFGEVG
                     LSGEVRHVPHADKRLAEAKKLGFDGAIGPLVKTGKKLTGLHGVSDVRSALNQFLEKD"
     gene            2126..2368
                     /locus_tag="VN778_00805"
     CDS             2126..2368
                     /locus_tag="VN778_00805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49552.1"
                     /translation="MQVGSKTYSGFNTGSGAGQFKLLLQNSMREDPPGGGKRSGYPFP
                     AGGNDMIFRYVAAYEPKSASHAGTNGPIIVPGTTVK"
     gene            complement(2393..>4879)
                     /locus_tag="VN778_00810"
     CDS             complement(2393..>4879)
                     /locus_tag="VN778_00810"
                     /inference="COORDINATES: protein motif:HMM:NF013106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HXR49553.1"
                     /translation="REYSKDDILTGYLNIAPYGGVEYGCEAAANDYFHKSCKDLNLAE
                     STMLAAIPQSPSLYSPYSSTQWNPDAGNYFSQQALAGRQMYILSQMVKQGYITQAQAD
                     AAKQVNVLAEVQPLQSKYAGIKAPYFVEAAKQELEQTYGSSTVQRGGWKVITTLDLNL
                     QNQAEQIVANNLPNVERRTAGLADEEAVVLEDIQTGQMKALVGGTDFSNADHGQNNYA
                     AGVLVSPGSSFKPYDYTTLINNNNNVGAGSVMYDTEGPLPENGYQCTNHALPKNGGNC
                     LADYDFLQPGPITLRYALGGSRNIPAVKAMLEAVPNDNTANKTASINKVISTASAMMD
                     NTYLQSKHQKTYNCYQQGVDVNNATPSDTTQCYAASAIGDGAYLHLDDHVNGLATLAR
                     EGVAIPRTYIMKITDAANKNVYTWKQPKGTQVVKQDAAYIVDNMASDPKASYLPGSCT
                     DTNCTKLYQGGYKFQRDNGWTFAVKTGTTNYGYDGLMASWSAKYAVISWVGNHTRNVD
                     ISSRTGTAMEYLTEPLTRGMMEAAHANVKPIAWTQPKDIKVAPAFIQRTHIHYGDQEP
                     GPTNDLYPSWYVGGGSSKNSSATIDKVSGKLATSCTPASAKEYVSNGNAASWNVDIFH
                     GGKASVGNSGVNNSTSSTSSATDDVHNCNDSPPTITLTAPSDCNTSCTITATVTQGTH
                     PLTDSQYPLYPGTVTFTLNGQTLHTASVSDSPSTVSFTYNPSSSGSGNLTATVTDSVL
                     YSGSDSATLNFTGASSLALAITKTGTILHLLWSGATGTTTGYANGPSSGSCSSSGTSC
                     DITGLPAGTYSVYAQDSSGNKSSTQTISTP"
BASE COUNT         1089 a         1178 c         1417 g         1195 t
ORIGIN      
        1 tgttcggtac cgaggtggct agcttgccga caggcttaga gcggtattat gagcgctact
       61 ttacgaatcg ggcagcagtc gctgcatata cggctcagta tcagaccgag tttacatcgc
      121 ggcaggcgac cgtggcccaa gatgacgctg agcttaccag cctgaagggg caaataacct
      181 cagccgaaaa tgcgctcaaa gccaaagaaa cagctatcaa tgcccaaaag tctcaactgc
      241 tcagtgagcg taacagtggt aacgtcgatg cttataatgc gggtgtacct ggctacaaca
      301 gcttgataga tggctacaac agggaagctc agaccgtgca aagcctgatc aaccagtaca
      361 atcagctggt agccagtcgc aacgccgtag cactggaaga agaccagcta gtaaatgatt
      421 taagtagcgt cagcccgatt aagcagtgac cggccgcgtt agcagccata tgatagactg
      481 gataatacct taaaggagga gttttggcta gaaaagcgtc gacccgatac gtctgtagca
      541 attgtggtgc catgcaaagt acctggagcg gccgctgccc gcagtgtggt gagtggaata
      601 cgcttgaaga gcaattgagc gccgaaactt tagctcgagg tgttaagagc ggccgggcat
      661 tagtagcaca gcccgtcgat aaatcgatcg ccgctgacaa tcaacgcttg gtcaccggta
      721 tggcagaagt ggacgacgtg ctggggggcg gtatcgtcac tggcagcgtc aaccttatcg
      781 ccggacagcc gggcattggt aaaagcacgc tgctgctgca gctggcgtat gcggtggctg
      841 ctaatgttcc cgtactctat gtcagcgggg aagagtcggc gcatcagatt gggatgcggg
      901 cagggcgcct tggaacgctt cggccgcagt tgcagctggc taccagtacg tcggctgacg
      961 atattgcggc gactattgcc ggcggtgcgt ataaattggt tattattgac tccatccaga
     1021 ctgttacttg cagcacaatc acctcggcag ccgggacagt ttcacagatt acaaatagca
     1081 cgcagttact aactatggct gctaagcaga gtgacacggc aatcgttttg gtcggccatg
     1141 ttactaaaga aggttcgatt gccggaccaa aggtgctgga gcatgtcgtc gacgtggtct
     1201 tgcagctaga aggtgaccgc tacggcggct tcaaagtgct cagagcggcc aaaaaccgct
     1261 ttggctccac taacgaagct ggcatcttcg agatggtgga gcaggggcta caacccgtcg
     1321 aaaacccctc agcagcactg ctggcagaac gtcagattag cgatggttca gttgtgctgg
     1381 cgacactgga aggcactcgt cctttgttgg tcgaagtcca agctttggtc aatcgtacaa
     1441 gttatgggta tccgaaacgg gccgccagtg gctttgatct gaaccggctg aatttacttg
     1501 tcgccatgtt ggagcggcgc actaagctta atctgagcga gcaagacatt tatataaata
     1561 ttgttggcgg tatccagatt cgtgagccag ccgcagatct cgcgatctgt atggctatcg
     1621 gctcggcggc caaagggctg cagctcaaga aaaacgctgc ggtcttcggc gaagttggcc
     1681 tttccggaga ggttcgccat gtaccgcacg ctgataagcg tctagccgag gcgaaaaaac
     1741 tgggttttga cggcgctatt ggcccccttg tcaaaaccgg caaaaagtta accggcttgc
     1801 acggtgtgag cgatgttcgg tcggccttaa accagtttct cgaaaaagac tagaagccat
     1861 tgcgggcaat tttgggttga cagacatccc tagctactac tattgtaggc ttaaacccta
     1921 acacactaaa tgtgcaatat aaagaccact cccggaaaac attgatgagc cggctcacgc
     1981 gccttcccgg gctcaagccc ctggtcgcta ctctattcat actccttttt gccagtcgcc
     2041 gggtgccaac attgaccctt ctgctggctt ccacacctgg actttttggg aaaatggaga
     2101 cacgtttacc acgtatatag acggtgtgca ggtcggtagc aagacgtatt ctggcttcaa
     2161 caccggcagt ggggctgggc agtttaagct gcttctgcaa aactcaatgc gggaagatcc
     2221 accaggtggt ggcaagcgat ccggctatcc attcccggca ggcggtaatg acatgatctt
     2281 ccgctacgtg gctgcttatg agcctaagag cgcgagtcat gccggcacca acggccctat
     2341 catcgttcct ggcaccacag taaagtaacg cggtggtacg gccaaaaaga gactaaggtg
     2401 tgcttatggt ctgagtagag gacttgttgc cgctgctgtc ttgggcgtag acactgtagg
     2461 tgcctgcagg taagccagtt atatcgcaac tggtgccgga gctgctacag ctccctgaag
     2521 agggaccgtt agcgtaaccg gtggtggtgc ccgttgcccc gctccacagg agatgcagta
     2581 ttgtccccgt tttggttatt gccagcgcca gggagctggc gcccgtaaaa tttagcgtgg
     2641 cactgtcgct tccggagtac aagacgctat ctgtcacggt ggctgtcaag ttcccgcttc
     2701 ctgaggagga ggggttatag gtgaaggaaa ccgtactggg ggagtcgctg acgctcgctg
     2761 tgtggagagt ttggccattc agtgtgaaag tgacggtccc gggatagagc ggatactgag
     2821 agtcggtcaa tggatgtgta ccctgtgtga cggtagcagt gatcgtacag ctggtattac
     2881 agtctgaagg agctgtgagg gtaatggtcg gggggctgtc gttacagttg tggacatcat
     2941 cggtggccga ggaggtgctg ctggtgctgt tattgacgcc actattgccc acactcgctt
     3001 taccgccatg gaagatatcg acgttccacg aggctgcgtt gccgttactg acgtactctt
     3061 tggcggaagc cggtgtacag ctggtcgcca gtttgccgga gactttgtcg atagtggccg
     3121 acgagttctt gctgctgcca ccgccgacat accaggatgg atagagatcg ttggtaggtc
     3181 ctggttcttg gtcgccatag tgaatatggg tgcgctggat gaaggctgga gcgactttga
     3241 tgtctttagg ctgtgtccaa gcaatcggct tgacgttggc gtgagcggct tccatcatgc
     3301 cgcgagtcag aggctcggtt aagtattcca tggctgtgcc agttcgggag gaaatgtcaa
     3361 catttcgcgt gtggttacca acccaagaga tcacggcata tttcgcggac cagctggcca
     3421 tcaaaccgtc gtagccgtag ttggtagtac cggttttgac ggcgaaggtc cacccattat
     3481 cgcgctgaaa cttataaccg ccttggtata gcttggtgca gttggtgtca gtacagctgc
     3541 cgggcaagta gctggctttc gggtcagaag ccatgttgtc gacaatgtaa gcagcatctt
     3601 gtttaaccac ttgcgtgcct tttggttgtt tccaggtgta gacatttttg ttggcagcgt
     3661 cagtaatttt catgatgtac gtgcgtggaa tagcgactcc ttctcgagct agcgtggcca
     3721 ggccattgac atggtcgtcc aggtgcaagt atgcgccgtc accaatggct gaggctgcat
     3781 aacactgtgt agtatcggaa ggagtagcgt tattgacgtc cacgccttgc tgatagcagt
     3841 tataggtttt ctggtgctta ctttgtagat aggtgttgtc catcatggct gaggctgtgg
     3901 agattacttt attgatggag gcggttttat tggctgtgtt gtcattaggt acagcttcga
     3961 gcatggcctt gacggccgga atattgcgtg agccgccgag ggcgtagcgc aaagttatcg
     4021 gacctggctg caagaagtca tagtccgcta aacagttacc gccattcttt ggtaacgcat
     4081 ggttggtaca ctggtagccg ttttccggta gcggaccttc agtgtcatac attaccgagc
     4141 cagcaccgac attgttattg ttattgatca aggtggtgta atcgtacggc ttaaagcttg
     4201 aaccgggcga tacaagtaca ccggcggcgt agttattctg tccgtgatcg gcattactaa
     4261 aatcagtgcc accgaccagc gctttcatct ggccggtctg aatatcttct agaacaaccg
     4321 cttcctcatc ggccagcccg gcagtgcgac gctcgacatt cggtaaatta ttggccacga
     4381 tttgttcggc ctggttttgc agattcaaat caagggtggt gataactttc cagccgccgc
     4441 gttgtacggt tgatgaacca tatgtttgtt ccagttcttg cttggcggcc tccacgaagt
     4501 agggcgcttt gataccggca tacttgcttt gcagcggctg gacctccgcg agaacgttca
     4561 cttgcttggc ggcatcggcc tgggcctgag taatataacc ctgcttaacc atttggctta
     4621 agatatacat ttggcggccg gcaagtgctt gctgactgaa gtagttaccg gcatctggat
     4681 tccattgcgt actactgtaa ggtgagtaga gactgggtga ctgcgggatg gccgccagca
     4741 tggtagactc ggccaaattt aagtctttgc agctcttatg gaaataatcg ttagcggccg
     4801 cttcacagcc atactcgaca ccgccgtacg gtgcaatgtt caggtagccg gttagtatgt
     4861 catctttaga gtattcgcg
//
LOCUS       DATNGP010000032         2827 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_1332018, whole genome shotgun sequence.
ACCESSION   DATNGP010000032 DATNGP010000000
VERSION     DATNGP010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2827
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1332018"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..833)
                     /locus_tag="VN778_00815"
     CDS             complement(<1..833)
                     /locus_tag="VN778_00815"
                     /inference="COORDINATES: protein motif:HMM:NF012236.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-transporting two-sector ATPase"
                     /protein_id="HXR49554.1"
                     /translation="MSSSKHFEQLIQQGNPVGEVIAVEAFLVKVRGLQPCSVHALIMF
                     EDGSKGFVHQVLTDHVVILHLGTEKLRIGLVAVVQHQELVSKVGKDFIGRVVSVTGDP
                     LDGKGPIAADATWPVFNTAPPLFERHAVEDQLESGVSAIDALFPIVRGQRMALLGDSK
                     SGKSTLVTQLTINQKNTNQIVVYCLIAKRRSDVDTLLTRLKENGGMEKAIVVVSTMFE
                     SLIMSYIAPYVACSMAEYLWQKCDQDTIIIYDDLTSHAHAYREVALLSGVSPGRDSYP
                     G"
     gene            complement(830..1381)
                     /locus_tag="VN778_00820"
     CDS             complement(830..1381)
                     /locus_tag="VN778_00820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49555.1"
                     /translation="MAPEVAAAEAPKQLTGLPVLVIGPVDIGRLSRELAMIDEALLQL
                     GLRKGGTEIKMPKTSQLLDKVIELNKLNLLQQTDRQILQEFLAMIDKQAPVIHISFSA
                     DPPVAFLEKLMAWLRHEIHPLLLVRIGLQPTIGAGCVVRTINQQFDFSLKKDFSTKRD
                     LLKQALKMDSAPAPEPAPEVVAA"
     gene            complement(1371..2000)
                     /locus_tag="VN778_00825"
     CDS             complement(1371..2000)
                     /locus_tag="VN778_00825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49556.1"
                     /translation="MTIWWLVLILGFLGAAGVAFIWLAVRIGNTGGNKGGKAQQSDSL
                     EEAAKQDVEHVFNEEFREELRNRGRLHFEKIIGENAMFLQQDLRLTTSQLNEYMKTEI
                     TRKLQEEFNKYEQSIMDAKQLAVESIQKTNTAIDEQRQLLSQQVQSEIVAEKQQLLEH
                     FEANMADIVNHYVLAAIGNQIDLNDQLEYILGDLEANKNAIIEDIQHGA"
     gene            complement(2074..>2827)
                     /locus_tag="VN778_00830"
     CDS             complement(2074..>2827)
                     /locus_tag="VN778_00830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49557.1"
                     /translation="GVTQSYNADTSVVQTGMLVELKPKDPTTVVPLPDSDIKHMLGVV
                     VPQNNAAIVLTPQNVTTQQVLVATTGHYNVLATNQNVPIKVGDYLTISALDGVAMKAS
                     EAQSEVIGKAAGNFSGTANVIGQISLKDTLGHTSQVALGRIPLDITIAQNPLAAKSSD
                     YVPSFLSKAAITVASKPVSAARIYLSMVLLFITAVFTGNMLYSGIRSGMIAVGRNPLS
                     KKSIMRSLIETVIAGLIIFVAGVFAVYLLLKL"
BASE COUNT          649 a          766 c          716 g          696 t
ORIGIN      
        1 tcaccaggat atgagtcacg gcccgggctc acaccggata gcagcgcaac ctcgcgataa
       61 gcatgcgcat gactggtcaa gtcgtcgtaa atgataatcg tgtcttggtc gcacttctgc
      121 cacaaatact cggccattga acaggccacg tatggcgcga tatagctcat gatcaacgac
      181 tcgaacatcg tggagacgac gacgatggcc ttttccatgc cgccattttc tttcagacgc
      241 gttagcaagg tatcgacgtc cgaccggcgc ttagcgatca gacaataaac cacgatttga
      301 ttggtatttt tctggttgat cgtcagctgc gttaccagcg tacttttacc tgatttactg
      361 tcgccaagca gcgccatacg ctggccgcgg acgattggga agagggcatc gatagccgaa
      421 accccacttt ctagctgatc ctcaactgca tggcgctcaa acaggggtgg cgccgtattg
      481 aacaccggcc aggtcgcatc cgccgcaatc ggccctttgc cgtcgagcgg atcgccggtt
      541 accgatacca cccggccgat aaaatccttg ccgactttgc tcaccaactc ctggtgctga
      601 acgaccgcga ctaaaccaat gcgcaatttt tcggtaccca agtgcaagat cacgacatgg
      661 tcagtcagta cctggtgcac aaagccctta ctgccgtctt caaacatgat taaggcatgg
      721 acggagcaag gctgcaaccc acgcactttc accaaaaacg cttcgacggc aatcacttcg
      781 ccaaccggat taccctgttg aataagctgc tcaaaatgct tactggaact catgccgcta
      841 ccacctcggg agctggctca ggcgcgggcg cgctgtccat cttcaaggct tgcttgagca
      901 aatcccgctt tgtagaaaag tctttcttaa gggaaaagtc gaattgctga ttgatcgtcc
      961 tgacgacgca gccggcacca atggtcggct gcagaccgat acggacgagg agcagcgggt
     1021 gtatctcgtg gcgcagccaa gccatcagct tttccaggaa ggccaccggc gggtcggcgc
     1081 tgaagctgat gtgaataacc ggtgcctgct tatcgatcat ggccaaaaat tcttggagta
     1141 tctgccggtc ggtttgttgc aataaattta gcttattaag ctcgatgact ttgtccagca
     1201 gctggctggt tttaggcatc ttgatttcag tgccaccttt gcggaggccc agctgcaaaa
     1261 gcgcctcatc aatcatggcc agctcccggc tcaagcgccc aatatcgact gggccgataa
     1321 ccagcaccgg cagtccggtc aactgttttg gtgcttcagc ggcggccacc tcaggcgcca
     1381 tgttgaatat cctcaataat ggcgttcttg ttggcttcca gatcacctag aatatattcc
     1441 agctgatcat tcaaatcaat ctgattgccg atagccgcta gcacgtagtg gttgacgatg
     1501 tccgccatgt tagcttcaaa atgctccaat aactgctgct tttcggcgac aatttctgat
     1561 tggacttgct gactgaggag ctgccgttgc tcgtcaatcg ccgtgttggt tttttggatt
     1621 gattcgacgg ccaactgctt ggcgtccatg atggattgct cgtacttgtt gaattcctct
     1681 tgcagcttcc gagtaatctc tgttttcatg tactcattga gctgcgacgt ggtgaggcgc
     1741 aaatcttgtt gcaggaacat tgcattttca ccaatgatct tctcgaaatg cagacgcccg
     1801 cggttgcgca attcttctcg gaattcttcg ttgaagacgt gctcgacatc ttgcttggcg
     1861 gcttcttcga gcgagtcgga ttgttgggcc ttcccgccct tattgccacc agtgttccca
     1921 atcctcacgg ccagccaaat aaaagcaacg ccggctgcgc ccaaaaatcc caggattaac
     1981 accagccacc agattgtcat gtttgtttac ccaccatagc tattgtttca gactcaggtt
     2041 ctaaaagctg cacggaatct ccgttccaaa ctgctagagc tttagcaata agtataccgc
     2101 aaatacccct gcaacaaaaa taattagacc cgcaatcact gtctcaatca agctgcgcat
     2161 aattgacttc ttagataagg ggttgcgacc gaccgcgatc ataccactgc gaataccact
     2221 gtagagcatg tttccggtaa aaacggccgt gatgaacagc agcaccatgg agagataaat
     2281 ccgagcggca ctgaccggct tggaggcgac cgttatggca gctttggaca aaaacgaagg
     2341 gacatagtcc gacgattttg cggccaaggg gttttgagca atagtaatgt cgagcggtat
     2401 ccgcccgagt gccacttggg aagtatgacc cagcgtatct ttaaggctaa tctggccgat
     2461 gacattcgcc gtgccgctaa aattcccggc ggctttgccg ataacctcgg attgcgcctc
     2521 actggccttc atggctacgc cgtcaagcgc cgagatagtc aggtaatcgc ccacttttat
     2581 gggaacgttt tggttagtgg ccaggacgtt atagtggccg gtcgtggcta ctagcacttg
     2641 ttgagtagtc acgttttgcg gagtcaggac gatagccgca ttgttttggg gcaccacgac
     2701 gcccagcata tgtttgatgt cactgtccgg cagcggtacg acggtagttg gatctttcgg
     2761 cttcaattcc acgagcatgc ccgtttgcac gacagaagtg tcggcgttgt agctttgggt
     2821 gacgccg
//
LOCUS       DATNGP010000033         2911 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_1024192, whole genome shotgun sequence.
ACCESSION   DATNGP010000033 DATNGP010000000
VERSION     DATNGP010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2911
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1024192"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..270)
                     /locus_tag="VN778_00835"
     CDS             complement(<1..270)
                     /locus_tag="VN778_00835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49558.1"
                     /translation="MKLTATVVTLIVVAGVVILTDNLKPKATTIASHLTPSVSTSTTS
                     STPTTTNSTSSSSTGSFKDGIYSATADYFVPNSDQRIKVTVTLSKG"
     gene            464..2290
                     /gene="typA"
                     /locus_tag="VN778_00840"
     CDS             464..2290
                     /gene="typA"
                     /locus_tag="VN778_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010164233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HXR49559.1"
                     /translation="MSKIQDPTKIRNIAVIAHVDHGKTTLVDGLLKQSQTFRENQAEM
                     SQELIMDSGDQERERGITITAKVTAVQYGDYRINIIDTPGHADFSGEVERTLNMADGC
                     LLIVDAQEGPMPQTKFVLSKALTAELKPIVIINKIDKPGARLAEVEDELADLFLELAV
                     HEDQLHYPVYYAIGREGKAWQQLPSDAQAETDLADIFEAIIHEIPAPHTELNKPFQML
                     VTALAWDSYKGKYAIGRITRGQVKPGDQVCICKKDGSQTKARVDNVFMSHGLNKFEVA
                     GGVAGDIVQVTGVADVQIGETLADPEQPEALSVIEIEAPTLKIYLGPNTSPVKGREGQ
                     FTTSRQIGERLEKELETNVGLRVAEAGIGYDVSGRGELHLSVLIETMRREGYEFEVGR
                     PQVVTKEENGEMVEPVEELLVEVPAEHVGAVQMELGQRKATLKEQFANPKGMTKLVYE
                     LPTRALLGLRNILMTNTKGTIIMNSLMIGYQPLGSALKQLRNGVLLAYETGVTTPYAL
                     QNAEARGTIFVGPAEQVYAGQIIGLNTRAEDMEINVCKAKHLTNMRSSSSDGVVQLTP
                     PTILSLEQCLDFIENDELLEVTPENLRLRKRELDPNKRKHLK"
     gene            2331..2852
                     /locus_tag="VN778_00845"
     CDS             2331..2852
                     /locus_tag="VN778_00845"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HXR49560.1"
                     /translation="MSKKSLPSKAMDDTSEDFESLYEANKPLIYRFMFWRTKDEMLAQ
                     DLTSNVFEKAWRTRKSFTDGSSKAWLYRIARTTLIDYWRKKKEVPDDGTLASKVVSDM
                     AELGEVFDRTLAVEELHKAVMKLPVEMYEVVRLRFIEGKSARETADHLHISEANVRVI
                     QYRALKKLRSYLQ"
BASE COUNT          771 a          682 c          806 g          652 t
ORIGIN      
        1 gcccttactt aaagtgacgg tgacttttat gcgctggtcg ctgttaggca caaagtagtc
       61 ggcggtagcc gagtagatgc cgtctttgaa agagccggtg cttgaggagc tggtagagtt
      121 ggtagttgtt ggtgtcgacg atgtcgtgct ggttgatacg gaaggagtca ggtgactagc
      181 gatggtggtg gccttcggtt tcaggttatc agtaaggata accacgccag caacgacaat
      241 aagtgtcacc accgttgccg tcagcttcat tttggcctgt ttttgataaa cgttcagtat
      301 gaactcctca ttactattaa ttgtatagca caaccttaac gaggaatact taaagaaaac
      361 ttaaagcttg ctgatagcga taggtgatta cgtttaggtg ggcaggaagt tcttcttatg
      421 gtataattta tggtgaaagg gagcgcggta ggcgctttat tttatgtcaa agatacaaga
      481 tccgaccaaa attcggaata ttgcggttat tgcgcacgtc gaccacggga aaactacctt
      541 ggtggacggt ttgctgaagc agtcgcagac gtttcgtgaa aatcaagcgg agatgagcca
      601 agagctgatc atggatagtg gtgatcaaga gcgtgagcgc ggcattacca ttacggccaa
      661 ggtcacggcg gtgcagtatg gtgactaccg catcaacatt atcgatacgc cgggccacgc
      721 tgactttagc ggcgaagttg aacgtacgct gaatatggcc gatggctgtc tgctgatcgt
      781 cgatgcccag gaagggccaa tgcctcagac caagttcgtg ctcagtaaag ccctgaccgc
      841 cgagctcaaa ccaatagtta ttattaacaa aattgataag cccggcgcgc gcctggccga
      901 agtagaagac gagctagccg acttgtttct cgagctcgct gtccacgaag accagctgca
      961 ttatcctgtg tattacgcga tcggacggga aggcaaggct tggcagcaat tgccctctga
     1021 tgcccaggcg gagactgatc tggcagatat ttttgaagct ataattcacg aaatccccgc
     1081 gccgcacacc gagctcaata agcctttcca aatgctggtg acggcactgg cgtgggacag
     1141 ctacaagggt aagtatgcca tcggccggat tacccgtggc caagttaaac caggggatca
     1201 agtgtgtatc tgtaaaaagg atggctcgca aaccaaagcc cgtgttgaca atgtcttcat
     1261 gagtcatggg ctgaacaaat ttgaagttgc cggtggcgtg gctggcgaca tcgtacaagt
     1321 caccggcgta gcagacgttc aaatcggcga aaccctggcc gatcccgagc agcccgaggc
     1381 cttgtcggtc atagaaatcg aggcgccgac tttgaaaatt tacctcggtc caaacaccag
     1441 tccggtaaag ggccgggaag ggcagtttac aactagccgt caaatcggag agcgtttaga
     1501 gaaggagttg gagaccaatg tcggtttgcg ggtggccgaa gctggtatcg gctacgacgt
     1561 tagtggtcgg ggcgagctcc acctcagcgt actgattgaa acaatgcggc gagaaggtta
     1621 tgaatttgaa gtgggccgcc cgcaagtcgt caccaaagag gaaaacggtg aaatggtgga
     1681 gccggtcgag gagcttttgg tagaagtacc ggctgaacat gtcggagccg tgcaaatgga
     1741 gctgggccag cgtaaagcga ccctcaaaga gcaatttgct aaccccaaag gcatgacgaa
     1801 gctggtctac gagctaccaa cccgagccct gcttggttta cgtaacattt tgatgaccaa
     1861 caccaaaggt acgattatca tgaatagtct catgattggc taccagccgc tcggcagtgc
     1921 cctgaagcag ctgcggaacg gcgttttgct tgcctacgaa acgggtgtta ctacgccgta
     1981 tgcgctgcaa aatgccgaag cccgcggcac aatttttgtc ggtcccgccg agcaagtcta
     2041 tgccggccaa ataattggtc tcaacacgcg ggccgaggac atggagatta atgtctgcaa
     2101 agccaagcac ttaaccaata tgcgcagctc gagcagcgat ggggtagtgc agctgacgcc
     2161 accaaccatt ctcagcctcg agcagtgcct ggatttcatt gaaaatgacg agctactgga
     2221 agttacacct gagaatctcc gtctccgcaa acgtgaactc gatcctaaca agcgaaaaca
     2281 cttgaaatag ctcggctaat gcggcctagt tgtaaaataa gtgtaacgtt atgagcaaga
     2341 aatcgttacc atcaaaggcc atggatgata catcggaaga ctttgagagc ctgtacgagg
     2401 caaataagcc tttgatatac cgtttcatgt tttggcgcac caaggatgaa atgctggcgc
     2461 aagatctcac gagcaatgtc ttcgagaaag cctggcgcac ccgcaagagt tttaccgatg
     2521 gctcatccaa ggcttggctg taccgcattg cgcgtaccac acttattgat tactggcgaa
     2581 agaagaaaga agtacctgac gatggcaccc tcgcttcgaa agtcgtcagt gacatggccg
     2641 agctcggtga ggtcttcgac cgaaccctag cggtggagga gcttcataaa gccgttatga
     2701 aattaccggt cgaaatgtac gaagtagtca gattgcgttt tatcgaaggc aaatctgctc
     2761 gcgagaccgc cgatcatttg catattagcg aggctaacgt ccgtgtcatc cagtaccgcg
     2821 cgctcaagaa gctgaggagc tatttacaat gagagagcct aaacagaatc agttacgaca
     2881 agaattactc caggctggcg ctaaggcgtc a
//
LOCUS       DATNGP010000034         3718 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_489162, whole genome shotgun sequence.
ACCESSION   DATNGP010000034 DATNGP010000000
VERSION     DATNGP010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3718
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_489162"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..402
                     /locus_tag="VN778_00850"
     rRNA            <1..402
                     /locus_tag="VN778_00850"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            679..793
                     /gene="rrf"
                     /locus_tag="VN778_00855"
     rRNA            679..793
                     /gene="rrf"
                     /locus_tag="VN778_00855"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            795..1517
                     /locus_tag="VN778_00860"
     CDS             795..1517
                     /locus_tag="VN778_00860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49561.1"
                     /translation="MKKASVRGGLFLLLDLSTPSHYNKRKQEIREGNGIMPDIDVKVN
                     SEEVNDGESFSPEPEHASESEPVAPHHEEPPVEHHDPVSSPSFTDKHGSPKMPNTNLL
                     VTGGLVLVIVILAIWALSLSSNNSKLKKDNSDLKNQVTQLNANPQAIIQKQTNDLISK
                     VSRLMQLPTGETPTVAEVSDAAAAKKQSAFFNNAQNGDKVLMYVNAHEAILYRPGTNK
                     IILVAPLTFSSTASSTSSTTKH"
     gene            1655..1849
                     /locus_tag="VN778_00865"
     CDS             1655..1849
                     /locus_tag="VN778_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49562.1"
                     /translation="MAGTAKGGRLAAQKNKKKYGPDFYARIGRMGGQKGHTGGFAAGE
                     AGRKRASYWGAVGGTRSRRH"
     gene            2031..2228
                     /locus_tag="VN778_00870"
     CDS             2031..2228
                     /locus_tag="VN778_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010164964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49563.1"
                     /translation="MAGTKAGGQAAAATNKQKYGSDFYAKIGAKGGKLGKTGGFFANR
                     ELAREAGRRGGRISRRTKKVA"
     gene            complement(2281..2784)
                     /locus_tag="VN778_00875"
     CDS             complement(2281..2784)
                     /locus_tag="VN778_00875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49564.1"
                     /translation="MDMQELIAKLVVAFPDLRFSAGKQFCWSPETGEVFYKHDDERET
                     APWSLLHETGHALLEHASYKADFELLRLEIAAWEKARDLAPSLGITIDEDHIEDCLDT
                     YRDWLHKRSICPTCTTKCLQQSDYVHYRCFNCHTVWRVTQNRFCRAYRSTKNVAPQPV
                     FHLETGF"
     gene            complement(2759..3598)
                     /locus_tag="VN778_00880"
     CDS             complement(2759..3598)
                     /locus_tag="VN778_00880"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HXR49565.1"
                     /translation="MYLGVDVGGTKTLVATLDGHGVITEKVRFPTPATYDEFLRQLKS
                     TIANLKADDFQTGGLAIPGEIDREHGRVLWLGNLPGWSPHLPLHVLSDVEKIADCPMV
                     LENDAKLGGLSEAMLRKDKFSKVLYVTVSTGIGTALISDGAIDTAFGDGGGRTLLLEH
                     RDKMVPWESFASGHAIVERYHHKAMEIEDPNTWRKICRDLAKGLIELIAITDPEVVVI
                     GGSVGVYFSRYGKILTAELKKYHLPLMPIPPLQAARRPEEAVVYGCYDLAKQVYGHAR
                     AHS"
BASE COUNT          940 a          905 c         1013 g          860 t
ORIGIN      
        1 gtttggcacc tcgatgtcgg ctcatctcat cctggggctg gagcaggtcc caagggtccg
       61 gctgttcgcc ggttaaagag gtacgtgagc tgggttcaga acgtcgtgag acagttcggt
      121 ttatatccgc tgtggacgca aggaaatttg agaggatttg accctagtac gagaggaccg
      181 ggttgaacgc accgctggtg tatcaattgt tctaccaaga gcattgttga gtagctatcg
      241 tgcggactag ataagcgctg aaagcatatt aagcacgaaa ctaacctcaa gattagattt
      301 ccctatgacc tccctgaaag acgatcaggt tgataggcgc aaggtggaag catggtaaca
      361 tgtgcagccg aagcgtacta atagaggcat tgacttttta cgacctttga tccgtcctgt
      421 ttacagggct taggatcaaa ggtagcaatt gactagttat tggtgcttaa tcggcaccaa
      481 taggtttgtc taaaagcaaa tctcgcaact ttgctaatcc gtgactaagt aacacatgga
      541 catcaatagc cctagacagt gtcaaatgac cactggctgg gaagagcagg gccggttccg
      601 ccataggcgg aatgcgaatt tatttcgcaa ggcccgatga gaaacctgcg atagcaggtg
      661 tctcattagg cgaagccttt tggtgcccat agcccgaggg caacacctgt tcccataccg
      721 aacacagcag ttaagcctcg gagcggttac aatacttggg gttcacccct gggaaaatag
      781 cacggtgcca aattatgaaa aaggcctccg ttcgcggagg cctttttctt ttgcttgacc
      841 tatccacacc ttcccactac aataagcgta agcaagaaat aagggaggga aatggcatca
      901 tgcctgacat tgatgtaaaa gtgaattctg aggaagttaa tgatggtgaa agcttttcgc
      961 ccgagccgga acatgcatcg gagtcagagc cagtagcccc tcaccacgag gaaccgccgg
     1021 ttgagcacca tgatccggtt agcagcccaa gcttcaccga taagcacggt tcgccgaaga
     1081 tgcctaacac gaatctgctg gttacgggcg ggctggttct ggtgatcgta atcctggcga
     1141 tctgggcgct tagcttgagc agtaataata gcaagctcaa gaaggacaat agtgacctga
     1201 agaatcaggt aactcagctc aacgccaacc cgcaggctat catccaaaag cagaccaatg
     1261 atctgatcag taaagttagc aggctcatgc agttgccgac cggggaaacc ccgacggtgg
     1321 ctgaagtcag cgatgcggcg gcggctaaga aacaatcagc tttcttcaat aacgctcaga
     1381 atggcgacaa agtattgatg tacgtcaatg cgcacgaagc gattctctat cggccgggca
     1441 cgaacaagat cattttagtg gcacctttga ccttcagcag caccgccagc agcacgagct
     1501 ctacgactaa gcactagatt gtctcctcgc cagcaagtgc gattttttac agccaagcat
     1561 tagctttcta agctaccata accatgttcg aggcgccggg ccaaactact aatgatggct
     1621 tcagtaattg cccctcggac aggaggatat atctatggca ggaactgcga aaggcggtcg
     1681 tttggctgca caaaagaaca agaagaagta tggacccgat ttctatgcac gcatcggtag
     1741 gatgggtgga caaaaaggac ataccggcgg ctttgcagcc ggtgaagcag ggcgcaagcg
     1801 cgctagctac tggggtgcgg ttggtggtac tcgttcccgg cgccactaat ttaacgacaa
     1861 ttctgaaata aaaattaaat gtaagtacaa gcttattgac ttataaacaa tagcgtgata
     1921 tcataagcgt agtttcgaaa gaaattaacg agggtttcct gatggcctta ggcctaggag
     1981 gccctttttg tttctctcgg gagagactaa tttaatggag gtactaacct atggcaggta
     2041 ctaaagcagg tggccaagca gcagccgcta ccaacaagca gaaatacggc tcggactttt
     2101 atgccaagat cggtgctaag ggcggtaagc tcggcaaaac tggcggcttt ttcgccaatc
     2161 gtgagctggc ccgtgaagcc ggtcgtcgtg gcggtcgcat ttcgcgccgc accaagaaag
     2221 ttgcctaagt agctctactc ttggaaggaa atctccgctt cggcggagat tttttgttgg
     2281 ctagaaaccg gtttcgaggt gaaaaaccgg ttggggcgcc acatttttgg tgctgcgata
     2341 tgcgcggcaa aaacggtttt gggtgacccg ccagacagtg tgacagttga agcaccgata
     2401 gtgcacgtag tcgctttgct ggaggcattt ggtagtacag gttgggcaga tgctgcgttt
     2461 gtgcagccag tcgcggtagg tatcaaggca atcttcgata tgatcttcgt caatggtgat
     2521 tcccaggcta ggcgctaagt cacgggcttt ttcccaggca gcgatttcca ggcgcaaaag
     2581 ctcgaagtcg gctttatagg aagcatgctc tagcagtgca tgaccggttt cgtggaggag
     2641 cgaccagggg gctgtttctc gttcgtcatc atgcttataa aaaacctcgc cagtctcggg
     2701 agaccagcaa aactgtttcc cggcgctgaa gcgcaaatcg gggaaggcaa ctaccagttt
     2761 agctatgagc tcttgcatgt ccatacacct gtttagctaa atcatagcag ccatagacca
     2821 cggcctcttc gggccgccga gcggcttgga gcgggggaat tggcatcaac ggcagatggt
     2881 acttcttcag ttcggccgtc agaattttgc cataacgaga aaagtaaaca ccgacactgc
     2941 cgccgataac aacgacttcc gggtcggtga tagcaattaa ttcgatgagg cctttggcga
     3001 ggtcgcggca aattttgcgc caggtgttcg ggtcttcgat ttccatggct ttgtggtggt
     3061 atcgctcaac gatagcgtgc ccggaggcga agctttccca cggcaccatt ttgtcgcgat
     3121 gttcgagcaa cagagtgcgt ccgccgccgt caccaaaggc ggtgtcgata gcgccgtcgc
     3181 tgatcagggc tgtgccgatg ccggtgctga ctgtcacata gagtactttc gaaaacttat
     3241 ctttacgcag catggcctct gacaggccgc cgagcttagc atcgttttcc agcaccatag
     3301 ggcagtcggc gatcttctcc acatcactca gcacgtggag tggcaaatgg ggactccacc
     3361 cgggtagatt gcccagccac aggacgcggc cgtgttcgcg gtcaatttcg ccggggatag
     3421 cgaggccccc cgtctggaag tcatcggctt tcagattcgc aattgtgctt ttcagctggc
     3481 gtaaaaattc gtcataggtt gcaggggtag ggaacctaac cttctcggtg atgacgccgt
     3541 gaccgtccaa ggtggctacc agcgtcttgg tgcctccgac atcaaccccc aaatacatat
     3601 agccagtgta taccgggaag gccgaatgtt caatgctccc caggcgagtc tctttacata
     3661 aggggtgtca tcagatataa taaaagtaat cttacgatca gcaagttaac cctaatta
//
LOCUS       DATNGP010000035         3924 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_2877040, whole genome shotgun sequence.
ACCESSION   DATNGP010000035 DATNGP010000000
VERSION     DATNGP010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3924
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2877040"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1791
                     /locus_tag="VN778_00885"
     CDS             <1..1791
                     /locus_tag="VN778_00885"
                     /inference="COORDINATES: protein motif:HMM:NF023266.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HXR49566.1"
                     /translation="PDFSVGAMENWGLMTFREQTMLVDPQTGSIESKQLVAIVVCHEL
                     SHQWFGNLVTMKWWDDLWLNESFANLMEYVGVHGLYPEWQIWEQFVSMDVASAKRRDS
                     LLDVQTIKTAVNHPDEIETLFDPSIVYAKGGTILRMLLTYVGEDAFRRGLKLYFDKHQ
                     YSNTEANDLWEALSQASGQAIADFMEGWLTRPGYPLIDIDWQPGEKIVSLKQQRFISN
                     PGLQAPPSKPWQVPLVASAQLDEPLLKTTSLRTALKPNENQPVIFNHEGSSYYLPYYR
                     NHEHLQSIVQGIHDDKVGTLDRLLLLDNYTLLQRGGQAETTDLLDLLKGYEGESSENV
                     WGAMSVALGEARKLTEDDEACEDKLNQLIASFATPLATKLGWDDESTDSAQTLRLRGL
                     AIALTAGAKVQAILDEGIGRFRACQKAADLPASTRGTVYFIASRHGEPKDFAKLLKMY
                     HSADSADEREEIAGGLSSTKRPKDTATLLDMFTTNDIRRQDLMHWYIWMLRNRYSREA
                     TWQWLVNNWDWAEREMSSDKTFSYFARYAGSVFSRPDELKKFQAFFEPKKTAVSLAHD
                     ITLATEEINARIAWRERNEATVKAWLKNYS"
     gene            complement(1788..2132)
                     /locus_tag="VN778_00890"
     CDS             complement(1788..2132)
                     /locus_tag="VN778_00890"
                     /inference="COORDINATES: protein motif:HMM:NF014119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HXR49567.1"
                     /translation="MNSTSIGLKAEAAAKVYLEMRGYKVLEQNWRRPRYEIDIVAEKG
                     GVKHFVEVKYRFNQEQGGGLEAITPTKLRQMHRAADAWVEENKWRGEYVLSAIEVEGP
                     NFIILSFIENAF"
     gene            complement(2138..2695)
                     /locus_tag="VN778_00895"
     CDS             complement(2138..2695)
                     /locus_tag="VN778_00895"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002710350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HXR49568.1"
                     /translation="MVTLGMDEVGRGCWAGPVVAGAVILGQPIAGLRDSKKLTKKQRQ
                     KMSAEIQIKAVAIGIGWVAAEEVDELGLSGAVKLAMQRAFEQITGDYDEIIIDGNVNF
                     LAENPKAKAVIKADDSVPAVSAASIIAKVARDNFMVERATAYPDYGFDKHVGYGTALH
                     LEKLKLYGTCELHRLSFKPVRALLQ"
     gene            2859..3098
                     /locus_tag="VN778_00900"
     CDS             2859..3098
                     /locus_tag="VN778_00900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49569.1"
                     /translation="MDLQKGGVHMTYWYPRQNINLVLPIKLLIEVEEAATEHHMSRTD
                     YIRHVLEKAVSGKYPEALEQARKNNPLKFYNLSDG"
     gene            complement(3102..3647)
                     /gene="rplS"
                     /locus_tag="VN778_00905"
     CDS             complement(3102..3647)
                     /gene="rplS"
                     /locus_tag="VN778_00905"
                     /inference="COORDINATES: protein motif:HMM:TIGR01024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HXR49570.1"
                     /translation="MQSVVQKINQQYKKSAVVDVRSGDTVRVHQKIREGNKERIQIFQ
                     GLVIRTDQKGSHTSRITVRRIASGIGVEKSFLLHSPLVVKVEVTKRSKVRRNYLSYMR
                     ARTGKSARMTGVEFDRDAVNAVHDEAAEAEEAKLREETAKEAAAKADEKAAEEAESEA
                     KVAAALASHDQTAESADSKAE"
BASE COUNT         1011 a          994 c          930 g          989 t
ORIGIN      
        1 cctgattttt cggttggcgc tatggaaaac tggggactca tgaccttccg tgagcaaact
       61 atgctggtcg accctcaaac cggcagcata gaaagtaagc agttagtcgc gatcgttgtt
      121 tgtcacgagt taagccatca atggttcggg aatttagtca ccatgaaatg gtgggatgat
      181 ctgtggctaa acgaaagctt tgccaatttg atggaatatg tcggcgtcca tggcctctac
      241 cctgaatggc aaatttggga acagttcgtc agcatggatg tagcaagcgc gaaacgacgt
      301 gatagcttgc ttgacgtgca gacgatcaag accgccgtta accatccgga tgaaattgag
      361 acactgtttg atccttcgat cgtgtatgcc aaggggggca ccattttgcg catgcttttg
      421 acatatgttg gcgaagatgc atttcgtcgc ggtctgaagt tatatttcga taagcaccag
      481 tacagcaaca ccgaggcgaa cgatctgtgg gaagctctgt cacaggcaag tggtcaggct
      541 atagctgact tcatggaggg ctggctgacg cggcccggtt atcctcttat tgacatcgac
      601 tggcagccag gagaaaaaat cgtaagcctc aagcagcagc gatttatcag caaccccggc
      661 ctgcaagctc ccccaagcaa accatggcag gtgcccctgg tagcttccgc ccagcttgac
      721 gagcccctac ttaagacaac ctcactacga acagccttga agccaaacga gaaccagcct
      781 gtcatattca atcacgaagg cagcagctat tatttgcctt actaccgtaa ccatgaacac
      841 cttcaaagca tagttcaagg tatacatgat gataaagtcg gcaccctaga ccgcttatta
      901 ctgttggata attacacgct cctgcagcgc ggtggtcagg ctgaaacaac cgatctgctc
      961 gatcttttaa agggctacga gggcgaaagc agcgaaaatg tgtggggcgc tatgtcggtc
     1021 gcccttggtg aggctcgcaa gcttaccgaa gatgacgaag cctgtgagga caagctcaat
     1081 caattgatcg ccagttttgc gacaccactg gctaccaaac ttggctggga tgacgaatct
     1141 accgatagcg cccagaccct ccgcttacgc ggtctcgcta tagcattaac tgccggtgcc
     1201 aaggttcagg ccatactcga cgagggtata ggtcgctttc gagcctgcca gaaagccgcc
     1261 gatctgcctg ccagcacgcg tggaacagtt tactttatag cctcacgcca cggcgagccg
     1321 aaagactttg ctaagctgct caaaatgtat cacagtgcgg acagcgccga tgagcgcgaa
     1381 gaaattgccg ggggattgag cagtaccaag cggccgaaag ataccgctac gctgctggat
     1441 atgtttacca ccaatgacat tcgccggcaa gatttgatgc attggtatat ctggatgctc
     1501 cgcaaccgct atagccgtga agcaacttgg cagtggttgg tcaataattg ggactgggcg
     1561 gagcgtgaaa tgtccagcga taaaaccttt agctactttg cccgttatgc cggcagcgtt
     1621 ttttcgcggc ccgacgaact caagaagttt caagctttct ttgagccaaa gaaaacagcc
     1681 gtatcattag ctcatgatat tacattggcc accgaagaga ttaatgctcg catcgcgtgg
     1741 cgcgagcgca acgaggctac cgtcaaagct tggttaaaaa attatagcta aaaagcgttt
     1801 tcaataaagc tcagaatgat gaagtttggg ccttcgactt caattgccga aagcacatat
     1861 tcgccccgcc atttgttttc ttcgacccag gcgtcggcgg cccgatgcat ttggcgcagc
     1921 ttggttggcg taatagcctc gagcccgcca ccctgctctt ggttgaagcg atattttacc
     1981 tccacaaaat gcttgacgcc gcctttttca gcaacgatat caatctcata acgcggtcga
     2041 cgccaatttt gttctagtac tttatagcca cgcatctcca gatagacttt ggcggctgct
     2101 tcagctttta acccgatact cgtggaattc ataccgttca ttgtaacagg gctctgacgg
     2161 gcttaaagct taggcggtgg agctcgcagg tgccatatag ctttagtttt tcaaggtgca
     2221 gtgccgtccc atagccgaca tgcttatcaa agccgtaatc cgggtacgcg gtcgcacgtt
     2281 caaccataaa attatctcgg gcgaccttgg caataatact tgccgcgctc actgccggta
     2341 cgctatcatc agctttgatg acggccttgg ccttggggtt ctcagccaag aagttaacat
     2401 taccatcaat aataatctca tcatagtccc cagtaatttg ctcgaatgcc cgctgcatag
     2461 ccagcttcac agcccctgaa aggcccagct catccacctc ttccgccgcc acccagccaa
     2521 tgccgatagc tacggcttta atttggattt cagctgacat tttttggcgc tgctttttgg
     2581 tcagcttctt ggaatctctt aaaccggcaa tgggctgacc taaaataact gcgccagcaa
     2641 ccactggccc agcccagcaa cctctaccaa cctcatccat gcccaaagtc accacataat
     2701 tattttacag tactgtcgcc gtgtaaaagc ttaactaaat cttaaattac agtcttttct
     2761 ttgtacttct tttggagcac tgtacatttt gtgtataaaa tctgtacaga aaatgtacag
     2821 tgccattttg aaaggtgagc tcttgaatag ccagagccgt ggatttacaa aaaggaggcg
     2881 tacacatgac atattggtat ccacgacaaa atattaattt ggtgctgcct atcaaattat
     2941 tgatcgaagt cgaggaggcc gccactgaac atcatatgtc taggactgat tatatacgcc
     3001 atgttttaga aaaagcagtt agtggcaaat acccagaggc ccttgagcaa gcacgcaaaa
     3061 ataatcctct gaaattttat aacctgagtg atggctagtg attattctgc tttggaatcg
     3121 gcagattcag ctgtctgatc atgtgaggcg agagcggcag caactttagc ttcgctttcg
     3181 gcttcctcag cagctttttc gtcagcttta gcagcggctt ctttggcagt ctcttcgcgt
     3241 agcttagcct cttcggcctc ggcagcttcg tcatggacgg cgttgacggc atcccggtca
     3301 aattcgacac ctgtcatgcg cgccgacttg ccggtacgag cacgcatata actaaggtaa
     3361 ttacgacgca ccttgctgcg ctttgtgact tcaaccttta ccaccagagg gctgtgaagt
     3421 agaaagctct tctccacacc gataccgcta gcgatacgcc ggacggtaat gcggctggta
     3481 tggctgcctt tttgatcggt acggataacc aagccttgaa aaatctggat gcgttccttg
     3541 ttgccttcgc ggatcttttg gtgcacacgt acagtgtcac cgctgcgaac gtcgacaacg
     3601 gcagacttct tgtattgctg gttaattttc tgaaccacac tttgcatatc cgacaaattg
     3661 tacaccattt aatctgttaa tgcaataata cgaacgttag aatccctaaa gctctaagaa
     3721 attatttaca cgagcggcga actatggttt ataatgcaag ccatatgtca gagagactca
     3781 acggaaatag tgaaagcccg cagcaagtgc acgtggcacg tggctatgtt atcaagcgac
     3841 aactcgggca aaaaactcag cggtatgtac actttaatcg aaaagttact ccagtcattg
     3901 ctcggcaaga tcacaagata atga
//
LOCUS       DATNGP010000036         3977 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_3920258, whole genome shotgun sequence.
ACCESSION   DATNGP010000036 DATNGP010000000
VERSION     DATNGP010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3977)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3977
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3920258"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            284..855
                     /locus_tag="VN778_00910"
                     /pseudo
     CDS             284..855
                     /locus_tag="VN778_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981743.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
     gene            1008..1493
                     /locus_tag="VN778_00915"
     CDS             1008..1493
                     /locus_tag="VN778_00915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49571.1"
                     /translation="MLTQPNDEDKIYINLISSLAGQYVSRRPHLIHLIKEVLTVMDLK
                     GARIVIERDMGRTIGNTDIVATSEKDTIYYAQPLKTEAYWRFAKNRYPQPSNKLTIIL
                     ERDADGNYEVSDTWIGPSVPPFPGDEHATAASKQYWETHALAQDTQSIQSKTITADCP
                     Y"
     gene            1637..2830
                     /locus_tag="VN778_00920"
     CDS             1637..2830
                     /locus_tag="VN778_00920"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HXR49572.1"
                     /translation="MSHYQYQRSDRYSRTSTINRNRSRSRQPKKAYIHPSKFVQSAKP
                     PTGEQYIPLHAFSDFQLHPLMQSNLTAMGFSNPSPIQDQTIPAGLSGRDVVGIANTGT
                     GKTAAFAIPVLNRLMADPRSVAIILAPTRELAQQIEDQCRQIAKGSGLTGALLIGGMS
                     MGPQLRELRARPRLIIGTPGRIKDHLERRSLSLANCNIVVLDEVDRMLDMGFVTDIRH
                     ILSQTNDTKQSFYFSATLDERVRGIITSFSHDPVHISVKSGETSENVEQNIVPYSSGS
                     DKIDKLHDLLIQDTVTKTLVFDDTHRSVEKLAKELGARGFSTDSIHGGKSQPQRQRVL
                     KKFKDSQIKVLVATDVAARGLDVSDITHVINYALPQSYDDYIHRIGRTGRAGKIGYAL
                     TFVEQ"
     gene            2906..3301
                     /locus_tag="VN778_00925"
     CDS             2906..3301
                     /locus_tag="VN778_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007920061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXR49573.1"
                     /translation="MLKAQAAFSGISVNDQAQAKQFYTEVLGLTLDNETMGLNLRLPG
                     GGALFIYAKEGHQPATFTVLNFVVDNIDEAVDELTGLGVSFEHYDNMPGKQDDKGILR
                     GRSANQGPDIAWFKDPAGNILSVLQNQLR"
     gene            3343..>3977
                     /locus_tag="VN778_00930"
     CDS             3343..>3977
                     /locus_tag="VN778_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011035033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXR49574.1"
                     /translation="MEKAIISAKDVSKRFGNIVAVDNISFDVQKGEIFAFLGPNGAGK
                     STTIKMLTTMLRPSEGELRLAGHDTAHERDSARKVFGIVFQDPSLDEELTAYENMDLH
                     AALYNIPKNSRAARIQELLQLVDLWDRKDSFIKTFSGGMRRRLEIARGLLHEPKVLFL
                     DEPTLGLDTQTRNLMWSYVKKLSEEKGMTIFFTTHYLDEAEEVAERIAIID"
BASE COUNT         1091 a          950 c          962 g          974 t
ORIGIN      
        1 ctctagcggc agtaagtacg ctgccgagcg gctgtaatag aggtatcctc atgtcggatc
       61 gcgcagggag tgtctcgctt gccggctcat cattaaaaga ctgatcgaaa ggtgtggcaa
      121 aaatctcggc attcagccca agcgggtgca ttacttatat ttcagcatat tgcgctaaaa
      181 taagtcaact ccttatgatt ctaacgtcaa cagggaggtt aatatttttg atctatcaat
      241 tcggctcaga aattacggaa gggaagtttg ctagaattga ggcatgcatc aattgtcgat
      301 tttaaagttc aagtctgcac ttgagtggga agcgtggctt gagaaaaatc atagtcagcc
      361 agatggcgtg tggctcaaat tagctaaaaa ggactccggc aagattacta ttcagcacaa
      421 agaagccttg ctcagcgctt tgtgttacgg ctggatagat gcccaggcta acaaatttga
      481 cgatacttat tatctgatta aattctgtcc gcgccgacca cgcagtgttt ggtctcagca
      541 taatcgcgac tacgtgcaga agttaattgc tgaagggcgc atgcaacctg ccggattggc
      601 ggaaattaaa gccgccaaag ccgatggccg atgggacaag gcctactcgc cccagagcac
      661 ggccgaaatg cccgtagatt ttatgaaagc cctggctgct aacccggcgg cgaccgaatt
      721 ctttgaaact ctaaacaaag ctaaccgcta cgccattatt tggcgcgcct gcaccactcc
      781 aaacgagctg aaacacgagc cagaaacatc gaaaagttcg tagcgatgct ggctaaccat
      841 aaaaagttcc attaaatttc agcaggtttt ataaccttgc acccggcaaa tacttctacc
      901 ggtttgaaca ataatagtct taattatccg taataaatga ttaaaaacga gcactaactc
      961 ctcaaacggc gtatactaaa gagtaagagc tacgcatgta caggtttatg ttaacccaac
     1021 ccaatgatga agataagatt tatattaatc ttatttcatc tttggctgga caatatgtca
     1081 gtcggcggcc ccatctcata cacctcatta aggaagtgct tactgtgatg gatttaaaag
     1141 gtgcacggat agtgattgaa cgggatatgg gtcgcactat tggcaatacc gatattgtgg
     1201 ctacgagcga gaaggacacg atttactatg cccagccgct taaaaccgaa gcttattggc
     1261 gctttgccaa aaatcgttac ccgcaaccca gcaataagtt aacaattatc ctcgagcgag
     1321 atgccgacgg caactacgaa gtcagtgata cgtggatcgg tccttcggtt cccccattcc
     1381 ctggtgacga acatgccacg gcggccagca agcagtattg ggagacccat gccctcgcgc
     1441 aagatacaca gagcatacaa tccaaaacaa ttaccgcgga ctgtccctac taaccattgt
     1501 gctaaacatt gcgtattaat tatgattgtg ctaaaatgat agtattgttt ctaatgaatc
     1561 ctgaggtatt tgcgaactcg atggtcattg cagacaatcg attcatcaaa tactaactac
     1621 aaaggattca ttttttatgt ctcattatca gtatcagcgt agcgaccgct atagccgcac
     1681 ctcaactatt aatcgtaatc gcagccgcag ccgtcaaccc aagaaggctt atattcatcc
     1741 gtcaaagttt gtgcagagcg ctaagcctcc taccggtgag caatacattc ccctgcatgc
     1801 tttttctgat tttcagctgc acccactaat gcagagcaac cttaccgcta tgggctttag
     1861 caatccgtcg cctatccagg atcagacgat tccagccggg ctgagcggta gagacgtggt
     1921 aggcattgcc aataccggta ccggcaagac agcggcgttt gccataccag tccttaatcg
     1981 gcttatggcc gacccccgta gtgtggcgat cattcttgct cccacccggg aactagcaca
     2041 gcagattgaa gatcagtgtc ggcaaatcgc taaaggcagc ggcttgaccg gcgcactgct
     2101 gatcggcggc atgtccatgg gaccgcagct cagagagctc agagcacgtc cgcgattgat
     2161 tattggcacg ccgggtcgga tcaaggatca ccttgaacgc cgctcgctta gtctggcaaa
     2221 ctgcaatatt gtggtcctcg atgaagttga ccgtatgctc gacatgggtt ttgtcactga
     2281 catccgccat attctgagtc aaaccaatga cacaaaacag agcttttatt tttcggcaac
     2341 gctcgacgaa cgggttcgcg gcatcattac cagcttttct catgaccccg tacacatttc
     2401 ggtcaagtcc ggcgagacga gcgaaaatgt cgagcagaac atcgtacctt acagctcagg
     2461 tagtgacaaa atcgataagc tgcatgactt gctgatacag gatacggtta cgaaaacctt
     2521 ggtttttgat gacacccatc gcagtgtcga gaaactagcg aaagaactgg gagctcgagg
     2581 cttctcgact gactcgattc atgggggcaa atcacagccc caacgacagc gggtactaaa
     2641 gaagtttaaa gacagtcaga tcaaggtgtt ggtggcaaca gatgtcgcgg ctcgcggtct
     2701 tgatgtttcc gacatcacgc atgtcattaa ttatgccttg ccgcaatcct acgatgacta
     2761 catacatcgt attggccgta ccggccgcgc aggtaaaatc ggttacgctc tgactttcgt
     2821 tgaacagtag aagttctcta gtgttagcga gcacattgac ctttctttcc ccgacgtgct
     2881 attgttcggg aaaaggaggg ttattatgtt gaaagcacag gcagcattta gtggaatctc
     2941 ggtaaacgac caggcgcagg ctaagcaatt ttatactgaa gtattaggtc ttacgctgga
     3001 caatgaaaca atgggcttga atttgcgtct gcctggtggc ggagctttgt ttatttacgc
     3061 caaagagggc catcagccgg caaccttcac cgtattaaat ttcgtcgttg ataatatcga
     3121 tgaagcggtt gatgaattga cagggctggg cgtcagcttc gagcactatg acaacatgcc
     3181 agggaaacaa gacgacaaag gtatcctacg tggccgcagt gccaatcaag gtccggacat
     3241 agcttggttc aaagaccccg ccggcaatat actttcagtt ctgcaaaatc agcttcgctg
     3301 atatacaaaa tatagcaagc acgttataat ggacgcgaat atatggaaaa agctatcatc
     3361 tcggcgaagg atgtgtctaa gcgctttggc aacattgtgg ctgttgacaa catttctttt
     3421 gacgtccaga agggcgagat ttttgcgttc ttaggtccga acggggcagg taagagcacg
     3481 actatcaaga tgctgaccac tatgttgcgg cccagtgagg gcgagcttcg gctggcggga
     3541 catgacacgg cgcacgaacg ggattcagcc cgaaaagtgt ttggcatcgt gtttcaagat
     3601 cctagcctgg acgaagagtt aacggcttac gaaaatatgg acctgcacgc cgctctttat
     3661 aatattccca aaaattcgcg cgcagcccgc atccaggaat tgctgcaatt ggtggatttg
     3721 tgggatcgca aagattcatt catcaaaact ttttcgggcg gcatgcgtcg gcgactggaa
     3781 atcgcgcgcg ggttgctaca cgaacctaaa gtgctgtttt tggatgagcc gaccctgggc
     3841 cttgacaccc agacccgtaa cttaatgtgg agttacgtta aaaaattaag cgaagaaaag
     3901 ggtatgacca ttttctttac cacgcactat ctagatgaag cagaagaggt tgccgagcgc
     3961 atcgccatca tcgacca
//
LOCUS       DATNGP010000037         2868 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_902195, whole genome shotgun sequence.
ACCESSION   DATNGP010000037 DATNGP010000000
VERSION     DATNGP010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2868
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_902195"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..254
                     /gene="rplL"
                     /locus_tag="VN778_00935"
     CDS             <1..254
                     /gene="rplL"
                     /locus_tag="VN778_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010164764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HXR49575.1"
                     /translation="AAPADGAAAADEGKSEYDVMLKDAGAQKVAVIKAVKDLTGLGLG
                     EAKALVDGAPKVVLEHAAKDAAESAKKALEEAGATVELV"
     gene            321..1184
                     /locus_tag="VN778_00940"
     CDS             321..1184
                     /locus_tag="VN778_00940"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HXR49576.1"
                     /translation="MHSQHTNPEIIKQETELVAKQTGSSAITLNDTGWDSRVYSVDDG
                     RFFFKFPRSEKIQGRYAQEIAALELAKHIDGVTVPDVRWYDPQNRFFGYQGVPGTLLD
                     NVVSSLSTHQKTQIGKQIGTFLAEFHQQTFPGIRTKTIADEIAQFQEWYQPAIPVLRA
                     AYTATEYGHLDQLVQETWPKRIEKLGRVGALCHGDLHLNNMLLTPDNALGIIDFGDVA
                     EYDQSKDFVDLDDPDIEEAAIQTYGDTPRLREKIAVRKKIISIISLTYNIKKERTESV
                     QKWLELIRQDL"
     gene            1321..1830
                     /locus_tag="VN778_00945"
     CDS             1321..1830
                     /locus_tag="VN778_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015642089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49577.1"
                     /translation="MADVPEKQVKRLRALIQEAETNLAAANELLISLVGDDEKVQARV
                     TEDVLGKVIEGVFDGQNMVGSDSKTYPVPANYASKSKLVQGDILKLTIAEDGAFLYKQ
                     IGPIPRKQVVGTLKLEGGHYFVTVGTREFRILLASVTYFKAKPGDQVSVNVPEDDSQA
                     EWAALEAAL"
     gene            1949..>2868
                     /gene="dnaX"
                     /locus_tag="VN778_00950"
     CDS             1949..>2868
                     /gene="dnaX"
                     /locus_tag="VN778_00950"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR02397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HXR49578.1"
                     /translation="MGQALYRKYRTKTFAETVGQEHITKTLENAIQQGKISHAYLLTG
                     PRGTGKTSIARILAHEINNLPYDDQAHLDIIEIDAASNRRIDEIRDLRDRVNTAPASA
                     KYKVYIIDEVHMLTKEAFNALLKTLEEPPAHVVFILATTEAHKLPETIISRTQRYTFR
                     PVPLDQVTAHLKFIAASEKITIDDDALALIAKHGEGSFRDSISLLDQSSSVAAHVTHA
                     DVERILGLAPEVHIQQLLQALSAGTSSAVVTTLSELYNLGFEPAMVARQLGDCLRQTV
                     LDSTAADPQASLALLKQLLDVPAARSPKQL"
BASE COUNT          783 a          780 c          694 g          611 t
ORIGIN      
        1 ccgctgctcc tgctgatggc gctgctgctg ccgacgaagg caagagcgaa tacgacgtta
       61 tgctgaaaga tgcaggcgct cagaaggtag ccgttattaa ggctgttaag gacctgaccg
      121 gtcttggtct tggcgaagct aaggctctcg ttgatggtgc tccaaaggtt gtgcttgagc
      181 acgccgctaa ggacgccgca gaaagcgcaa agaaggctct tgaagaggcc ggcgctactg
      241 ttgaactcgt ctagttttag atagttctat aacaaaacac cgctccagaa cgagcggtgt
      301 tttgttatac tggcttgcac atgcacagcc aacataccaa tccggaaatc atcaagcagg
      361 aaaccgaact agtcgccaaa caaaccggct cgtcagccat taccctcaac gacaccggtt
      421 gggatagccg cgtctactct gtagacgacg gcaggttctt tttcaagttt cctcgttccg
      481 agaaaatcca aggccgctat gctcaggaaa ttgccgctct tgagcttgcc aagcacatcg
      541 atggcgtcac cgtaccagac gtacgctggt acgaccccca aaaccgcttc tttggctacc
      601 agggcgtccc aggcaccttg ctggacaatg tagtaagttc tttatccaca catcaaaaaa
      661 cgcagatcgg taaacaaatt ggcacattcc tcgccgaatt ccaccagcag acgtttccgg
      721 gcattcgcac caaaactata gccgacgaga tcgctcagtt ccaggagtgg taccaacccg
      781 ccatacccgt tcttcgtgcc gcttataccg ctacagaata tggacatctg gaccaactag
      841 tccaggagac atggcccaag cgtatagaaa aactaggtcg agtgggggcg ctatgccacg
      901 gcgaccttca cctaaataat atgctgctga cgccagataa cgcgcttggc attatcgact
      961 ttggcgacgt tgcagagtat gatcagtcta aggatttcgt tgaccttgat gatcctgaca
     1021 ttgaagaagc agctatccaa acgtacggtg atacacccag gctgcgagaa aagattgccg
     1081 tccgcaagaa gattattagt attatttcgc tgacctataa catcaagaaa gagcgcaccg
     1141 aatcagtaca aaaatggctt gagctgattc gccaggatct atagacttca cttatccaca
     1201 gattttctgt aaaccaaatg gtatttttga cttgccgagg ccggtatggt acggttaggg
     1261 taaggtgtgg agcaaaaagg ttagaaaaac tactcaggtg agatagaaac gagaataaat
     1321 atggcagatg taccagagaa acaagtcaaa cggctccggg ctcttattca agaggccgag
     1381 actaatctag ctgccgccaa cgaactgctt atcagcttag tgggcgacga cgaaaaggta
     1441 caggcccgtg ttaccgaaga cgtacttggc aaggttatag aaggcgtttt cgacggccag
     1501 aacatggtcg gctcagacag caagacctat ccggtgcccg ctaactacgc cagcaaatcc
     1561 aagctcgttc agggcgacat cctaaagctc accattgccg aagacggcgc ttttctctac
     1621 aagcaaatcg gcccaatccc acgtaagcag gttgttggaa cactcaaatt agagggtggc
     1681 cattactttg taactgttgg cacccgtgaa ttccgcattc tactggccag cgtcacctac
     1741 ttcaaggcta agccaggcga ccaagtgagc gtcaacgttc cagaggacga ttctcaagcc
     1801 gagtgggctg cactcgaagc agccctataa aaacagccgg attcaccggc tgttttcata
     1861 gcaacttatg gtactgcact aaccccgtca ttagggtact ttctacagca gacggggccg
     1921 gctaaacagc gtatactact agctaactat ggggcaagca ctctaccgga aataccggac
     1981 taaaacgttc gccgaaactg ttggccaaga gcatattaca aagaccttgg aaaatgccat
     2041 ccagcagggc aagattagtc atgcctatct gttaacaggc ccacgcggca caggcaagac
     2101 ctctattgcg cgtatcttag cgcacgagat caacaattta ccttacgacg accaagcaca
     2161 cttggacatc attgaaattg acgctgctag caaccgccgc atcgatgaga tccgcgacct
     2221 gcgtgaccgc gtcaacactg cgccagccag tgccaaatac aaagtgtaca taattgatga
     2281 agtacatatg ctcaccaagg aagcattcaa tgcgctccta aaaacgctcg aggagccgcc
     2341 agcccacgta gtgttcatcc tggcaaccac cgaagcccac aagctaccag aaacgattat
     2401 cagccgcacg cagcgctaca ccttccgacc ggtgccactc gatcaggtta cagcccatct
     2461 caaatttatt gccgcctccg aaaagattac tatcgatgat gatgcactgg cactgatcgc
     2521 caaacacgga gaagggtcct ttcgagacag cattagccta ctggaccaat ccagcagtgt
     2581 cgccgcccac gtcacccacg cagatgtcga gcgcatcctt ggtctagcac cagaagtaca
     2641 catacagcag ttgctgcagg cgttgtcggc agggactagc agtgccgttg ttactacgct
     2701 aagcgaactg tacaatttgg gctttgagcc ggctatggtt gcgcgccagc taggcgactg
     2761 cctccgccaa accgtactag atagtactgc tgcggacccc caagccagtt tagcgctctt
     2821 aaaacagttg ctcgatgtgc cagcggcacg cagtccgaag cagctttt
//
LOCUS       DATNGP010000038         3356 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_397541, whole genome shotgun sequence.
ACCESSION   DATNGP010000038 DATNGP010000000
VERSION     DATNGP010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3356
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_397541"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..527
                     /locus_tag="VN778_00955"
     CDS             <1..527
                     /locus_tag="VN778_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49579.1"
                     /translation="ATPEALPESPAAPLQQANTGNSQAPITWTASEYIANQKSSNWYA
                     MLAVAAILIGFVVWLLTRDFFPTVAVLIGVILLGYYAGRQPREQTYALDDQGLTIGGR
                     HMAYHEFRSFTVVQDGAFLNVELTPLKRFAVEATMYVDPSDEKRIISRLSQYLPMEEA
                     KTSFTDSLMRRIKF"
     gene            complement(524..1846)
                     /locus_tag="VN778_00960"
     CDS             complement(524..1846)
                     /locus_tag="VN778_00960"
                     /inference="COORDINATES: protein motif:HMM:NF006579.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HXR49580.1"
                     /translation="MDKYKQLLPGYIKNKSISTDAAFQDGIVATVEWLRQQFTDSGFE
                     VTVVEGYDNPIVVASYVADPSYKTCLIYGHYDVQPADESEGWDSDPFVATERGGRFYC
                     RGAIDNKGQNLVHVVSIIELVKAGKLGYNVKFMIEGNEETGSPNLEQFLKDKKDLLKA
                     DFVMISDGELTANLPTIELGFRGGFNLTLTVTTSSTDLHSGMFGGAAPSSAHELAKLI
                     AGLYDKDNRITIPGFYDGVDEITDDIRKNNERIPFSEEEYQKLSGTKGRLGEPGYDFY
                     TQTALRPSIQVTGIKSGYTGDGYRNSIPASSTAKINFRLVLHQDPNAVIKLFTDYVKN
                     AVPDYVDVVVEPSDPYDGIKLSPDSEYIAKAYQTLQEVYGSKPLYKYVGGGIPVVTYF
                     NDLMGIPTVSVPLANEDCYMHAANENFTLDCVEKGLAFSRKFFGRDQV"
     gene            complement(1986..2120)
                     /locus_tag="VN778_00965"
     CDS             complement(1986..2120)
                     /locus_tag="VN778_00965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49581.1"
                     /translation="MNAFDEEMLMENFDRWNDRDKRERFLRRMEHKLHAKELRYPRGA
                     "
     gene            2267..3208
                     /locus_tag="VN778_00970"
     CDS             2267..3208
                     /locus_tag="VN778_00970"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HXR49582.1"
                     /translation="MVRTLAHGIQALMQKLDRWQQRRQLIGFVFAVVRKYLDDEAGAR
                     AALLAYYGFLSLFPLLLILTTVFRIVLHNDPGLSGEIIHSAVSYFPALGQSLQENVHS
                     LDKAGLTLVVGILLALFGARGVADVLQTSLDHIWQVPYSHRRHFPQSLLRSLGIVIVG
                     GVCLAVAPVIFGYALNFAPSRVVSVISALLTAVVMFWLLVWVIKFGSSARQPLHRIWL
                     GAFVAVVALSVLQAIGGFIVGHELQRLDTLYGTFALVLGIIFWLYLQTQLLLLALEVD
                     PVRAFKLAPRSFQSPLTDGDRAAYQLYHSRGKFHDGL"
BASE COUNT          673 a          883 c          930 g          870 t
ORIGIN      
        1 cggctactcc tgaagcgttg cccgagagcc cagcagcacc cctccaacag gccaataccg
       61 gaaatagcca ggcgccaatt acctggacag cgtcagagta tattgccaat caaaagtcga
      121 gtaattggta tgccatgctt gccgtagccg ctattcttat cggtttcgtc gtctggttgc
      181 tgacgcgcga ctttttcccg acggttgctg tgcttattgg cgtgattctc ctgggctatt
      241 atgccggccg acagccgcgt gaacagacct atgcactcga cgaccaagga ctgaccattg
      301 gcggccgtca catggcctac catgaattcc ggtcatttac ggttgtgcaa gacggagcgt
      361 ttctgaacgt cgaacttaca ccgctcaagc gttttgcggt tgaagctacg atgtacgttg
      421 atccaagcga tgaaaagcgg atcattagcc ggctgagtca atatttgccg atggaagaag
      481 ccaaaaccag ctttaccgat agcctgatgc gccgcatcaa gttctagact tggtcccgac
      541 cgaagaattt tcggctgaag gcgaggcctt tttccacgca atcaagcgta aagttctcgt
      601 tggcggcgtg catgtaacag tcctcgttgg ctagcggcac ggagacggtc ggaatgccca
      661 tcaagtcgtt gaagtatgtt accaccggga taccgccacc gacatatttg tatagcggtt
      721 tgctgccgta gacttcctga agtgtctggt aggctttcgc aatgtactca ctgtccgggc
      781 tcagcttgat accgtcgtag gggtcggatg gttcaacaac tacgtcaaca taatctggca
      841 cggcattctt cacgtagtcg gtaaatagct tgatgactgc atttggatcc tggtgtagta
      901 ccaggcggaa gttaattttg gccgtggagc tagccggaat gctgttgcgg tagccgtcac
      961 cggtgtagcc agacttaatg ccggtcactt ggatcgaggg ccgaagagcg gtttgggtgt
     1021 aaaagtcgta gcctggttcg cccaagcggc cttttgtccc agacagtttc tggtactcct
     1081 cttcactaaa cggaattcgc tcgttgtttt tacgaatatc gtcagtgatc tcatcgacgc
     1141 cgtcataaaa gccgggaatg gtgatccggt tgtctttgtc gtacaggccg gcaatgagct
     1201 ttgctagctc gtgggccgag ctgggagcgg cacccccaaa catgccggag tgtaggtcgg
     1261 tagaagaggt agtcacggtt agagtcaagt tgaagccgcc ccggaagccc agttcgatcg
     1321 tcggcagatt agcggtcagt tcgccgtctg aaatcatcac aaaatcggct ttgagcaggt
     1381 cttttttgtc tttgaggaat tgttcgaggt tcggcgagcc ggtctcttcg ttgccttcga
     1441 tcatgaactt gacgttgtag ccgagtttcc ctgctttgac tagctcgata attgatacta
     1501 cgtgcactaa gttctgaccc ttgttgtcga tggcgccgcg gcagtagaag cggccaccgc
     1561 gctcggtcgc aacgaatggg tcgctgtccc agccttcgct ttcgtcggcg ggttggacgt
     1621 cgtagtggcc gtagatcagg caggtcttgt acgagggatc agctacgtag ctggcaacca
     1681 cgatggggtt gtcataaccc tcgactactg tgacctcaaa gccactatca gtgaattgct
     1741 ggcgcaacca ctcgactgtc gccacgatgc catcctgaaa tgccgcatct gttgagatac
     1801 ttttgttttt gatgtaacca ggcagtaact gcttgtactt gtccataccg tatacctttc
     1861 gtatctagaa gtatacggag acgtatcacc cctgtaaata ggtgtacggg taggacccgt
     1921 ccaagatgac acaatacaaa caccacgtgt aaccgtggtg tttacccaga tcaagcggcg
     1981 gtttttcagg cgcctcgcgg gtagcgtagc tccttggcgt gcaacttatg ctccatgcgc
     2041 ctcaagaacc gttcgcgttt atcgcggtcg ttccagcggt cgaaattttc cattaacatc
     2101 tcttcatcaa atgcgttcat agtgctagtg tcccccgttt agccctaacg cccataagca
     2161 tagttcgttc ggctaaagat tggctgtaaa gtttttaacc gtgccgctgg gtcgagtcct
     2221 aaaaatgtat gcaagacgcc gctctggccg gtacaatagc agcataatgg tacggacgct
     2281 tgcccatggt atccaggcgc tgatgcaaaa gcttgatcgc tggcagcagc gccggcagct
     2341 cataggtttt gtatttgcgg tagttcggaa atatctagac gacgaagctg gggcacgcgc
     2401 agcactgctt gcctattacg gttttttgtc gctgtttccg ctgctgctca tcctaacgac
     2461 ggtgtttcgt atcgtactcc ataatgaccc gggcttgagc ggcgagatta tccattcggc
     2521 agtaagctat ttcccggcgc tcggtcagtc gctgcaagaa aacgtgcaca gtctcgacaa
     2581 ggcgggttta acactggtcg tgggcatcct cttggcgttg ttcggtgcgc gtggtgtggc
     2641 cgacgtactc cagacaagcc tggaccatat ctggcaggtg ccgtattcgc accgccgcca
     2701 cttcccgcag tcgctgctgc gtagtctcgg tatcgtcatt gtgggcggcg tttgcctggc
     2761 ggtagcgcct gttatcttcg gttacgcgct caactttgcg ccgagccggg tggtcagcgt
     2821 catttcagcg ctactgaccg ctgtggtgat gttctggctg ctcgtctggg ttatcaaatt
     2881 tggctcatcg gcgcgccagc cgctccaccg tatttggctg ggtgctttcg tggccgtcgt
     2941 ggctctgagc gttctgcagg ctatcggcgg ctttatcgtc ggtcacgagt tgcaacggct
     3001 cgatacgctg tacggcacgt ttgccctggt actgggaatt atcttttggc tctacctgca
     3061 gacgcagcta ctgctgctgg cgcttgaagt tgacccggtg cgggcgttca agctggcgcc
     3121 tcgctcgttc caatctcccc tgaccgatgg ggaccgtgcc gcctaccagc tctatcatag
     3181 ccgcggcaaa ttccacgacg ggctctaaac gatgtacact gttggaatga agctcgcccg
     3241 agactgccga actttctggg gacggtatcg gcgcccgtat ccagaggtga aggagatttg
     3301 taaccaatga agcttttcgc tttttggctg tttgattggc tcgatacatt tgtcga
//
LOCUS       DATNGP010000039         2949 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_656608, whole genome shotgun sequence.
ACCESSION   DATNGP010000039 DATNGP010000000
VERSION     DATNGP010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2949)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2949)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2949
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_656608"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..118)
                     /locus_tag="VN778_00975"
     CDS             complement(<1..118)
                     /locus_tag="VN778_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXR49583.1"
                     /translation="MRRLFSKQNRALLSELVRTDFKIRYQGSVLGYAWSLLRP"
     gene            157..1167
                     /locus_tag="VN778_00980"
     CDS             157..1167
                     /locus_tag="VN778_00980"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HXR49584.1"
                     /translation="MQKVLVVIPNWNGADDIRTCLDSLRQQTLQPHIVVVDNGSTDDS
                     VDIIEAEYPEVEIIRHTANKGYAGGVNPGFRVAIDRRFDYAAPLNNDAVADKNWLRYL
                     SDYLSAHHGVGIAASKLVSIDGAHIDSTGDQYTNWGLPYPRGRGETDIDKYDKHTAIL
                     GASGGSSMYRVSMLQKIGLFDEDFFAYYEDVDLSLRAQLAGWKVAFVPESIVYHQISA
                     TGSRIKGFFTLQTVKNLPWILIKDLPAGLFWRVLPRFLLAQLFFVGRAVLRRHGWYAL
                     KAITVTLWLMPKKLVERHRIQSTRKVPASYIWSIMTHDLPPQAANLRRLRAAWWKLQG
                     KR"
     gene            1164..2267
                     /locus_tag="VN778_00985"
     CDS             1164..2267
                     /locus_tag="VN778_00985"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HXR49585.1"
                     /translation="MKKIVIDAREWPTSTGRYVRELVEQLQEVDTDLSHRYYILMKPK
                     DLDSWEPKSKRFTKVASRYKEFTFAEQIGLFWQLLVIQPDLVHFAMVQQPILYFGKVV
                     TTMHDLTTLRFNNPSKNPLVFWFKQRVYVWVNFIAAHKSKAVIVPTEFVKDDVARNMR
                     TNSRKITVIYESGDTYPMKPEAVAELEDKQFIMYIGRPSPHKNLSRLIEAYGTLKQSM
                     PELHLALVGKRDALYKRHEQEVERLGIPDVHFTGFLSDARVRWLYEHAAVYVFPSLSE
                     GFGLPGLEAMANGAPVASSNATCLPEVYGDAALYFDPRNVDDMAGTIKRILTDEKLRA
                     RLISAGRKQVAKYSWRRMAEQTLEVYKRVLGER"
     gene            complement(2271..2771)
                     /locus_tag="VN778_00990"
     CDS             complement(2271..2771)
                     /locus_tag="VN778_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008578907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HXR49586.1"
                     /translation="MNDDQPTDAINSTDPGMEQAIVYAQKFLEDVLSFLGLNTVVYAT
                     HEDDVIQLNIPSTQMNGFLIGSHGDNMRSMQLLISSALKNQGYAYSRVNVDVAGYKQQ
                     RASRLADTAEAWVKRVSETREPMELRPMNAADRRVIHQLASERGLTTESIGEGRDRRV
                     VLKPAA"
     gene            complement(2768..>2949)
                     /locus_tag="VN778_00995"
     CDS             complement(2768..>2949)
                     /locus_tag="VN778_00995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49587.1"
                     /translation="AFWQQSRILREDETELEAIADKTEKRDLSAIVEAEVIASKDTSS
                     AASHTSKKRKRRRKK"
BASE COUNT          689 a          799 c          790 g          671 t
ORIGIN      
        1 gcggccgcag cagcgaccag gcatagccaa gcaccgaacc ttggtaacga atcttgaaat
       61 cggtgcggac cagctcactc aagagcgctc ggttttgctt cgagaaaagt cgcctcatgt
      121 tttagagtat tatagggtaa gaacgaactt tagtcgatgc aaaaagtact cgtagttatc
      181 ccaaactgga acggcgctga cgatatccgg acgtgcctgg attcgctgcg ccagcaaacg
      241 ctgcagccgc atattgttgt ggtggacaat ggctcaaccg acgattcggt agatattatc
      301 gaagctgaat atccagaagt ggaaattatc cggcatacgg caaacaaagg ctacgccggc
      361 ggcgttaacc cgggttttcg ggtagccata gaccgacgat ttgactatgc tgccccgctc
      421 aataatgatg ctgtggcgga caagaactgg ctgcgctact tgagcgacta tttgtcagcg
      481 caccatggcg taggaattgc ggcctccaag ctcgtcagca ttgacggtgc acatatcgac
      541 agcacgggag accagtatac caattggggc ttgccgtacc cccgcggacg tggtgagacc
      601 gatatcgaca aatatgacaa gcatactgct atcctcggag ccagcggcgg atcgagtatg
      661 taccgcgtga gcatgctgca gaaaatcggc ctgttcgatg aagatttttt tgcttactac
      721 gaagatgttg acttgagcct gcgggcacag ctggccggtt ggaaggttgc ctttgttccc
      781 gaatctatcg tctaccacca gatcagcgcc acgggcagca ggatcaaagg cttctttacc
      841 ctgcaaacgg tcaagaacct gccgtggatc ctcataaaag acctgccagc gggtctattc
      901 tggcgcgtgc ttccccgctt tttattggct cagctgttct ttgtcggccg agccgtactg
      961 cggcgccatg gttggtacgc cctaaaagca atcactgtaa ccctgtggct gatgcccaag
     1021 aagctggtcg aacgtcaccg cattcagtcg acacgcaagg tgcccgcgtc gtatatttgg
     1081 agtattatga cgcacgacct gccgccccaa gccgctaact tgcgccggct tcgggccgct
     1141 tggtggaagc tacagggcaa gcgatgaaga aaatcgtgat cgatgcccgc gaatggccga
     1201 ccagtactgg ccgctacgtg cgtgaactcg tcgaacagct gcaagaggtc gacaccgacc
     1261 tgtcgcaccg ctattacatc ctcatgaagc caaaagacct ggactcgtgg gaaccgaaga
     1321 gcaagcgctt taccaaagtt gccagccgct ataaggagtt tacctttgcc gagcagatcg
     1381 gtttgttctg gcagctactc gttatacaac ccgacctcgt gcattttgcc atggtccagc
     1441 agccaatttt gtactttggc aaagtcgtga ctacgatgca cgaccttact actttacggt
     1501 ttaataaccc atcgaaaaat ccgctggtat tctggttcaa gcaacgggta tatgtctggg
     1561 tcaatttcat agccgcccat aaatcgaaag ctgtcatcgt tcctacggag ttcgtaaaag
     1621 acgatgtggc ccgcaacatg cgcacgaata gccgcaagat caccgtaatc tatgaatctg
     1681 gcgacacgta tccgatgaaa ccagaagctg ttgctgagct ggaagacaag cagtttatca
     1741 tgtacattgg ccgcccttcg ccccataaaa acctaagccg gctcatcgaa gcatacggaa
     1801 cactgaagca atcaatgccg gaactccatt tagcactcgt aggcaaacgc gatgcgctct
     1861 ataagcgcca tgaacaagaa gttgaacggc tggggatacc agacgtacat tttaccggct
     1921 ttttgagtga tgcccgtgtc cgctggctgt atgagcatgc ggccgtctac gtcttcccga
     1981 gcctgagcga gggtttcggc ctgccagggt tagaggccat ggccaatggc gctccggtag
     2041 ccagcagtaa cgccacctgc ctgcccgaag tctacggcga cgctgctctc tactttgacc
     2101 cccgtaatgt cgatgacatg gccggcacga ttaaaaggat cctgactgat gaaaagctgc
     2161 gtgcccggct cataagcgcg ggacgtaagc aagttgctaa gtactcgtgg cggcgcatgg
     2221 ccgagcaaac gcttgaagtt tataaacggg tactcggcga acgctagacc ttaggcagcg
     2281 ggcttgagta caacgcggcg gtcgcggccc tcaccaatcg attcagtcgt taacccgcgt
     2341 tcgctcgcca gctggtgaat aacgcggcgg tcagcggcat tcattggccg cagctccatg
     2401 ggctcacggg tctcactaac ccgcttgacc cacgcttcgg cagtgtcagc caggcgcgag
     2461 gcccgctgct gcttgtagcc ggccacatcg acattgaccc gtgaataggc atagccctgg
     2521 ttcttgagcg cactactgat aagtagctgc atggaacgca tattgtcgcc gtgcgagcct
     2581 atcaaaaagc cattcatctg cgtcgatgga atattaagct ggatcacgtc atcttcgtgg
     2641 gtagcgtaaa cgacggtatt caaacccagg aaagacaata cgtcctcgag gaacttctgg
     2701 gcgtacacga tagcctgttc catccctggg tctgtgctgt ttattgcgtc agtaggttgg
     2761 tcgtcgttca tttttttcgt ctccttttac gcttttttga cgtatgagaa gctgctgatg
     2821 aggtgtcttt ggaggcaatg acttcggcct caacaatagc cgatagatcg cgcttctcgg
     2881 tcttgtcggc aatggcttcc agctcggtct cgtcctcacg caagatgcgc gactgttgcc
     2941 agaaagcga
//
LOCUS       DATNGP010000040         3037 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_1524194, whole genome shotgun sequence.
ACCESSION   DATNGP010000040 DATNGP010000000
VERSION     DATNGP010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3037
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1524194"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="VN778_01000"
     CDS             complement(<1..233)
                     /locus_tag="VN778_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HXR49588.1"
                     /translation="MNLERFITALGPEEVVHGASAGAATDVLDLAYDARTVRPGALFF
                     CVRGESADGHDFAADAVERGAVALVVERPLDLDV"
     gene            complement(325..2127)
                     /locus_tag="VN778_01005"
     CDS             complement(325..2127)
                     /locus_tag="VN778_01005"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HXR49589.1"
                     /translation="MFPANKNTLNQSVKRVRIWYGLLILVVAIFGVRLFYVQIIHYGY
                     YKTAALSDQLKQYQIPATRGIIEAHDGDNILPIVLNQQLYTLYADPTYVKKPDDAAGK
                     VAGIIGGDEAGYAKLMRTQDTRYVVLAKKLTTDQSNKLLGLKIPGLGTQAQDYRTYPQ
                     GSLAAQVLGFVNNDGVGEYGLEQAFNKQLTGKPGQVKAVTDVNGVPLAATKGNVETTP
                     TDGDKIVTSLDLSMQAQMEQILSQEYQKTHSQGLSAIIMDPNTGQIKAMANYPTFDPG
                     NYQDVSDSSVFENAAVTNPIEPGSTMKVLTTAAALDQGIINPNTTFYDPAHWLVDGFN
                     ITDIEEDGGPRTQSIASLLNLSLNTGATWLLMQMGGGQINTKARNAWYDYMSNHFRLG
                     QPTGVEQGYESDGLIPKPQDNGAGIDLTYANTSFGQAVQITALQMAAADSAVLNGGTY
                     YQPTLIDQTIDATGHATTNKPKVLKTHVVSPKVSQEMLPLMQYVVEQHLQEGFNYLNF
                     DQNNYTVGGKTGTAQVATSHGYSTTEYTASFVGMVPASHPRLVVLVKVDDPRGSIFGG
                     VVAAPAFASIARFDLQYLEVPPDAPKTLTSATTG"
     gene            complement(2195..2518)
                     /locus_tag="VN778_01010"
     CDS             complement(2195..2518)
                     /locus_tag="VN778_01010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49590.1"
                     /translation="MNRTRFSGRNQNSVSFQVKARTLGPVSNTIVLIVLACLLGLLYL
                     TQVTKTNAYGYQISALQAEQQRLTSQHDDLEVSSARLQSLSRVQNSAVAKSMVATAPS
                     GTVKN"
     gene            complement(2680..>3037)
                     /gene="mraW"
                     /locus_tag="VN778_01015"
     CDS             complement(2680..>3037)
                     /gene="mraW"
                     /locus_tag="VN778_01015"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011089350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="16S rRNA
                     (cytosine(1402)-N(4))-methyltransferase"
                     /protein_id="HXR49591.1"
                     /translation="AKRAWPGHSRVHPATRTFQALRIAVNDELAQLEKALPIWLELLN
                     PGGRLAIISFHSLEDRLVKKAFSEAAGNRYDAELQLLTKRPVTADRTELVHNPRARSA
                     KLRAAVKTKTREREMP"
BASE COUNT          613 a          817 c          856 g          751 t
ORIGIN      
        1 acgtcgaggt cgagcggccg ctcgacgacg agcgcgacgg cgccgcgctc caccgcgtcc
       61 gcggcgaagt cgtggccgtc cgcgctctcg ccgcgcacgc agaagaagag cgcgccgggc
      121 ctcaccgtcc gcgcgtcgta cgcgaggtcg aggacgtccg ttgcggcgcc ggccgacgcc
      181 ccgtgcacga cctcctccgg cccgagcgcc gtgatgaagc gctccaggtt catcgcggtc
      241 cgagtctagc gccctgttcc ggacggcccg cgtccccggc ggacacgtcc ggtgccaggt
      301 ccctggaacg gaagtgtgac gggatcagcc ggtggtcgcg ctggtgaggg tcttcggggc
      361 atccgggggg acctcgaggt actggaggtc gaacctcgcg atcgaggcga aggcgggggc
      421 ggcgacgacg ccgccgaaga tcgagccacg ggggtcgtcg accttcacca ggacgacgag
      481 gcgcgggtgc gaggcgggca ccatgccgac gaaggaggcc gtgtactcgg tggtcgagta
      541 cccgtgggag gtggccacct gggccgtgcc ggtcttgccg cccacagtgt aattattttg
      601 gtcaaagtta aggtaattga agccttcttg caagtgctgc tccaccacat attgcatgag
      661 cggcagcatt tcttggctga ctttcggtga taccacatga gtcttcagaa ccttcggttt
      721 gttcgtggtc gcgtggccgg tggcatcaat cgtttggtcg atcagcgttg gttgatagta
      781 cgtaccgcca ttcagcaccg ccgagtctgc ggccgccatt tgcagtgcgg taatttggac
      841 tgcctgaccg aaggaagtat tagcatatgt cagatcaatt cccgcgccat tatcttgagg
      901 cttgggaatc aacccatccg attcatatcc ctgttcaaca cctgtcggtt gcccaagccg
      961 gaagtgattg ctcatgtagt cgtaccaagc attgcgggcc ttagtattaa tttggccgcc
     1021 acccatctgc atcagcagcc aggttgcacc ggtgttcaaa gacaaattca gcaaactggc
     1081 aatgctctgg gtgcgcgggc cgccatcttc ttcaatatca gtaatattga agccatcgac
     1141 gagccagtgc gccggatcat aaaaagtcgt attggggtta attatacctt ggtccagggc
     1201 agctgcagtc gtcaggacct tcatcgtcga gcctggctca atcgggttgg taacagccgc
     1261 gttctcaaac acactggaat cactaacatc ttggtagtta ccggggtcaa atgtcgggta
     1321 attagccatg gctttgattt gaccagtatt cggatccatg atgatggcgc tgaggccttg
     1381 ggagtgtgtc ttttggtact cctgactaag aatttgttcc atctgggcct gcatggaaag
     1441 atccagcgag gttacgattt tgtcaccatc ggtcggcgtc gtctcaacgt tgcccttggt
     1501 cgcagccagt ggcacgccgt taacgtccgt aactgccttt acctgccctg gcttgcccgt
     1561 gagctgtttg ttaaaagctt gctccaggcc gtactcgccg acgccgtcat tgttcacaaa
     1621 acccaatact tgcgctgcca agctgccctg aggatacgtc cgatagtctt gcgcttgcgt
     1681 gccgagccct ggtattttta ggcccagcaa cttgttgctc tgatcggtgg ttaatttttt
     1741 agccagcacc acatagcgtg tatcttgggt gcgcattagc ttagcgtagc ccgcttcatc
     1801 accaccgatg atgccggcga ccttgcccgc agcatcgtca ggttttttga cataggtcgg
     1861 atcggcgtac agtgtataga gctgttggtt caagacaatc ggcaaaatat tgtcgccgtc
     1921 atgtgcttcg ataattccgc gagtagctgg tatctggtat tgcttgagct gatcgctcag
     1981 cgccgccgtc ttataataac catagtgaat gatctgcacg tagaacaaac gcacaccaaa
     2041 aatagcaacc accaaaatca gtagcccata ccagatgcgg acacgtttaa cggattgatt
     2101 gagcgtattt ttgtttgccg gaaacactag tttacattat acagtactaa gctttagttc
     2161 ttagaccttt gatagtagat cttagaacta aagcttagtt tttaactgta cctgaaggag
     2221 ctgtagccac catgcttttg gcgaccgcac tattttggac gcgcgacagc gattgcaagc
     2281 gagctgacga gacttccaga tcgtcgtgtt gactggtgag ccgttgttgc tcggcttgta
     2341 aagcactgat ctggtacccg taggcattgg tttttgtaac ttgtgtcaga tagagcaggc
     2401 ccagcaggca agccagtacg ataagtacaa tagtgttgct gaccggaccg agcgtccggg
     2461 ctttgacctg aaagctgacc gagttttgat tgcgccccga aaaacgggta cgattcatag
     2521 ctagcgagct tgagtatgtc atagttttat ctctgttttt atgtttatct tagtgctcca
     2581 gcccctgtaa atcgggactg gagccgcttc cgcctgcgat ctaagccact ttgacgtgga
     2641 tcttgtaggc gcgggagctg cttttgacct ggataggcat tatggcattt ccctttctct
     2701 tgtttttgtt ttcactgcgg cccggagttt agcgctccgt gcacgcggat tatggactag
     2761 ttcggtgcgg tcagccgtga ccggacgttt cgtaagcaac tgtaactcgg catcataccg
     2821 attaccggcc gcttctgaaa aggctttttt gaccagcctg tcttccaggc tgtgaaagct
     2881 aatgatcgct agcctgccac ctggattcaa taattccagc cagatcggca acgccttttc
     2941 cagctgcgcc agctcgtcat tgactgcaat gcgaagcgcc tgaaaggttc gtgtggccgg
     3001 atgaacccgg ctatggccgg gccaggctcg cttggca
//
LOCUS       DATNGP010000041         2133 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_3183639, whole genome shotgun sequence.
ACCESSION   DATNGP010000041 DATNGP010000000
VERSION     DATNGP010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3183639"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..855
                     /locus_tag="VN778_01020"
     CDS             <1..855
                     /locus_tag="VN778_01020"
                     /inference="COORDINATES: protein motif:HMM:NF012379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HXR49592.1"
                     /translation="LDQDFYLRISHELPLKRLLGAGFEKVYDIGPRFRNENYSDEHLP
                     EHIAMEWYWAYADWHDGTKFMTDMYRHVLQNTFGTLTFKLGNFDVDLSKDWEEWDYVD
                     TIQSRYGFNPLDVSLEVVKKALTDNGLEVEQTENKARGIDKLWKHIRKDVAGPVWLIN
                     TPTFISPLSKANPDRPETVQRFQPIIAGSELGNGFSELNDPLDQLQRFTEQQQMRDAG
                     DEEAMMLDIDYVEMLEYGMPPACGWGYSERVFWHFENVTAREGVPFPQLRHEVDEVTK
                     SIYPDLKL"
     gene            874..1299
                     /locus_tag="VN778_01025"
     CDS             874..1299
                     /locus_tag="VN778_01025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49593.1"
                     /translation="MPETASEALDMLHDIERAALPITDIEQKKEYYQEVVDPSYLEPV
                     VEHYLSIASQQVFTRNPNKGKSWTGRLNDRKFFAFVANCSYRYYESLGINGLVFAFVE
                     PSLVEVQPQHKATLKKLQFIAPVLGVTAFHNADFLAVEA"
     gene            1360..1854
                     /locus_tag="VN778_01030"
     CDS             1360..1854
                     /locus_tag="VN778_01030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49594.1"
                     /translation="MSPFEGLQISKLPLLRNLQTLQFAVNLIEDVEEKEAVARRGLNN
                     PVIAGILGQFLGIDASLAYGPQVSTSNKYSVFDSGLLFAARAQKVDYDCNYEIPVESI
                     VIEFPKPKQLVPESVLFYDEDGELPDRETIETLEETAAPEIKMVRVPVLAIRACAVEI
                     PRAA"
     gene            1889..>2133
                     /locus_tag="VN778_01035"
     CDS             1889..>2133
                     /locus_tag="VN778_01035"
                     /inference="COORDINATES: protein motif:HMM:NF018021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HXR49595.1"
                     /translation="MIEISDDEFQHLIDTALTELPGEHAKNIKNIAILYESEPTPEQR
                     VRLQLRHDQSLLGLYEGVPLSERQGMTRLLPDKITLF"
BASE COUNT          594 a          502 c          529 g          508 t
ORIGIN      
        1 ctggatcagg atttttatct gcgaatcagt cacgaattac ccctaaagcg actgctcggt
       61 gctggcttcg aaaaagttta cgatatcggc ccgcgcttcc gcaacgaaaa ctattcggac
      121 gaacatctgc cggaacacat cgccatggaa tggtactggg cttacgccga ctggcacgac
      181 ggaactaaat ttatgactga catgtaccga cacgtcctgc aaaatacttt cggtacgcta
      241 accttcaagc tcggcaattt cgatgtcgat ctctccaagg attgggagga atgggattat
      301 gtggacacta ttcaaagtcg ctacgggttt aacccgctgg acgtttcgct cgaagtggtc
      361 aagaaggctc ttactgataa tggccttgag gtggagcaga cagagaataa agcccgcggg
      421 atagacaagc tctggaagca tatccgcaaa gacgtggcgg gcccagtttg gctcatcaat
      481 acaccgacct ttatcagtcc gctgtcgaag gcaaatcctg accggccgga aaccgtacag
      541 cgcttccagc cgattattgc cggcagcgag ctcggtaatg gcttctctga actcaacgat
      601 ccgctcgacc agcttcagcg tttcaccgag cagcaacaga tgcgcgatgc cggcgacgaa
      661 gaggccatga tgctcgacat cgactatgtc gagatgcttg aatacggcat gccgcccgcc
      721 tgtggctggg gctacagcga acgcgtcttc tggcacttcg aaaacgtcac cgcccgcgaa
      781 ggcgtcccat ttccgcaact ccgccatgaa gttgacgaag tcaccaaatc gatttatcct
      841 gatctcaagt tgtaaatcat gagcactcct ttcatgccag agacagccag cgaggcttta
      901 gatatgcttc acgacattga acgtgcggcg ctgccgataa ctgatattga gcaaaagaaa
      961 gaatattacc aagaagtagt agacccatcc tatctggagc cagtagtcga gcattatctc
     1021 agcattgcca gccaacaggt atttacccga aatcctaaca aaggcaaaag ctggactggt
     1081 cgtctaaatg accgtaaatt tttcgctttt gtggccaatt gcagctaccg ttactatgaa
     1141 tccttaggga ttaacggcct ggtgttcgcc ttcgtcgagc cctctctggt tgaggtgcag
     1201 ccgcagcata aagcgacact taagaagttg caatttattg caccagtatt gggagttact
     1261 gctttccata acgccgactt tttagccgtc gaagcgtagc aatttcgaat acttggtggt
     1321 acagtaactc attatttgta tttgtgcgga ggaagaaaga tgtcaccttt tgaaggatta
     1381 cagataagca agctgccgtt actaagaaat ttgcaaacgc tacagtttgc ggtcaacctg
     1441 atagaagatg ttgaggaaaa agaagcagta gctagacgag gcttaaataa cccggtcata
     1501 gccggtattc tagggcagtt tttgggaata gatgccagtc tagcttatgg gccacaagtg
     1561 tcaacatcaa ataaatactc agtgttcgat agcggcttat tattcgccgc cagagcccag
     1621 aaagtagatt atgattgtaa ttatgaaata ccggttgaat ctatagtaat tgaatttccg
     1681 aagccgaaac aattggtgcc cgaaagtgta ttattttatg atgaagacgg cgagttaccg
     1741 gatagggaaa ccattgaaac tctcgaagaa acagcagctc cagaaatcaa aatggtaaga
     1801 gtgccggtct tggctattcg ggcttgtgcc gtggaaatac cgcgggcagc ctaggcgagg
     1861 acaacataat cctatacaat gtatagcaat gattgaaatt tcggacgatg aattccagca
     1921 tcttattgat acggcgctga ctgaattgcc aggcgagcac gccaaaaaca tcaaaaatat
     1981 tgcaatttta tacgaaagcg aaccaacgcc ggagcagcgg gtacgactcc agctgcgaca
     2041 tgatcagtcg ctgctgggtt tgtacgaagg cgttcctttg agcgagcgac aggggatgac
     2101 gcgactactg ccggataaga ttactctgtt caa
//
LOCUS       DATNGP010000042         8538 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_3237601, whole genome shotgun sequence.
ACCESSION   DATNGP010000042 DATNGP010000000
VERSION     DATNGP010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 8538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8538
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3237601"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..829)
                     /locus_tag="VN778_01040"
     CDS             complement(<1..829)
                     /locus_tag="VN778_01040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49596.1"
                     /translation="MAASSEISSVVGHMWPLIAGAGTLGGASKVASKTGIVQIIDADY
                     RGVRLRRGQPSMVPLNHTEVEKFLHGIAQPGLRGHLPFTRNILLVNIMNRRSIISTPV
                     DCSDGKQRNEGAQVIWGVKPEAPHPFRAIFKPARPDVKKGEPSTEPLKDIADGIFTGA
                     FRSVMGEVLRDPHGQPVVEGEFDQKGNLLDEDAVLQKRMIEKCAGKLLLYGCEIREFS
                     LIEDARSLGQFIVEGWGPQHNSGSNGANGREPMHSEAGKLAYLSEIAPELGQARASSS
                     "
     gene            complement(963..1715)
                     /locus_tag="VN778_01045"
     CDS             complement(963..1715)
                     /locus_tag="VN778_01045"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HXR49597.1"
                     /translation="MIETALIRVATLNIQHGSRGSYSDPGNPELVAEACRQINADVLG
                     LQEVDAGVPRSRKENLAAYAAKACGMDYRFAPTRTFRARGKVGNALLVRGEIKSYDSL
                     KLTGDWQRITVGNHKIPVIREPRNVLIGDVCVNGYDLAVGVIHAGGRRRREMLEHAAT
                     ALLFRPGPHILLGDYNVRLPYAHETLKPFAFTVLEGGPTSKSEGADEYDQQIDHIAVH
                     GLRAYGVEVMPVPVGDHQALVAEVKPVTLSVL"
     gene            1772..2143
                     /locus_tag="VN778_01050"
     CDS             1772..2143
                     /locus_tag="VN778_01050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49598.1"
                     /translation="MKTLENAFSLDLLKQRIHSLKLDGFVVYTTVDFPLKGEATYGAL
                     VMSVDDHASILQFVNELHDDQSLGPHEYLSDDIIREDRRYVETDSAYAALTNSEELCS
                     KLLDNALPDEYISLDNTSWYL"
     gene            complement(2152..3183)
                     /gene="mnmA"
                     /locus_tag="VN778_01055"
     CDS             complement(2152..3183)
                     /gene="mnmA"
                     /locus_tag="VN778_01055"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016476792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HXR49599.1"
                     /translation="MAKKVYVGMSGGVDSSVTAALLQEQGYDVTGVYMKNWSQDLPGF
                     VCPWKEDYQDAKRVAVQLGIDFKMFDFETEYRQKVVDYMVREYQAGRTPNPDVMCNQE
                     VKFKLFLEAALAEGADMIATGHYARIKDGQLFAGLDPAKDQSYFLYRVTEAALQKSLM
                     PIGELHKTEVRRLAKKLGLATADKKDSQGICFVGKVGIKEFLLNELGPQPRGPIIDQK
                     GGVIGEHDGAIFYTIGQRRGLNVGGGLPYYVSRKDMKANTIYVTTNLDDRGLWHKEVH
                     LQDLHWINQPPAEDKPYQVRLRYSGPLVECTVKGTILFLKDEQRGLAAGQSAVLYKDG
                     CVLGGGIIS"
     gene            complement(3214..4095)
                     /locus_tag="VN778_01060"
     CDS             complement(3214..4095)
                     /locus_tag="VN778_01060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HXR49600.1"
                     /translation="MKRRTQWLLVLGLSFLAFGSHSLASPFPPQSSSGSIGLQGEISS
                     AAPTRGATIAVPGNGAVFTNVPITVSGLCPTGLLVKIFSNNVFVGATYCASGSYSVQI
                     DLFSGQNDLVARVYDSLDQAGPDSNTVTVTFNDAQFLQFGTHVELSSAYAERGAPPSQ
                     EIDWPILLSGGTGPYAVSVDWGDGSPTDLMSVASAGTITIKHTYKTAGIYKVIVKATD
                     KNGGTAFLQLVGQATGAIQNNAKGGSGNAVIEHDVLWWPALAMFPLIFAAFWIGRRHE
                     LYSLRKRLEASRDEERA"
     gene            complement(4136..5140)
                     /locus_tag="VN778_01065"
     CDS             complement(4136..5140)
                     /locus_tag="VN778_01065"
                     /inference="COORDINATES: protein motif:HMM:NF017810.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HXR49601.1"
                     /translation="MRNTHVRRNIRLTLASIGTTLLVVMGGLMPTATAAGGNGLRVSP
                     VRSDLTINPGKSQTININVTNVTTVSATFQAIVNDFVANPDESGNPAIILDPNQSAPS
                     HGLKHYVQPIKNFTLAAGQQKSVPVTVAIPANAPGGGYFGAVRFAPANVSAAPNQNVT
                     LAGSVGSLILVKVPGNITDKLSLASFDVRDKKGDASSFFTSNKDITAAVRFQNEGNVQ
                     EEPFGKILLKNHSGKILATYELNNTYPPGNVLPDSIRRFNIALNKVGSFGQYKVEGNF
                     GYGSGGQLLSASTTFYVIPISVIIGFVLLVLLLLFLVFGLPRLIRAYNRRILRRAGRR
                     "
     gene            complement(5195..6256)
                     /locus_tag="VN778_01070"
     CDS             complement(5195..6256)
                     /locus_tag="VN778_01070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49602.1"
                     /translation="MSRSTQKSRRFLVGLVVAMFVLVGVGHYLTVPVAGAQIGQRAMQ
                     LSDNEVSKTSDYLLTFNLTTPGTLGSIVVQFCSNSALPGDPCTASSGFDDSAAVLASQ
                     TGQTGFTISGASTPNEIILTRAPAAATAGHVEYHFTGVTNPNASGSYFVRVQTYATAD
                     ATGPETDEGGIAYAIVNGPVQLNAEVPPYLIFCTGVTISGLNCANAEGDFIDFGELSS
                     SSARSGSSQMLVATNAGNGYNITVDGTTMTSGVNVIGALTANDVSRPGTDQFGMNLRA
                     NSTPAVGINPTGPGTGVPVANYNQPNFYQFNPGDTLVSNPTTDNYREFTASYIVNVPK
                     GQASGIYVSTLTYIALATF"
     gene            complement(6270..7616)
                     /locus_tag="VN778_01075"
     CDS             complement(6270..7616)
                     /locus_tag="VN778_01075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49603.1"
                     /translation="MNNLFSFDVRMARRVICAMLAALLFVSAAPAIFMGKAAANPNQL
                     QSRSIQMSDSAPSGNASITSGVGSGTSVTYRVSFTTTQAAASLVIDFCKEDPIIGDTC
                     TGPTGMNAASAAISTVASGGNVQGGGAWTVTAGASQVKLAQGSGSAMTAGAQQFDLTG
                     ITNPSSSNCTSPALACTFYARIYTYSGTSWGTYSSATNVGGDEDYGGIALSTASAIQI
                     TARVQEQLTFCVSDADPATWATPSNDDCADQQVGQNPPVVTLGVAVGAGQPVLDPSRV
                     DTGSIYSQVSTNATHGAVIDMRNSNLLCGGLSADSGTTCAIPAVNSGNSTGAPIAAGT
                     AAFGMCSVTYTPSSPVDSTIVGSMTPQAPYFHSGNSCTDATPWYGMDTTTAAANGGAP
                     ASNVGNVETTFGSIVAATTTPVHHADNHYVFAATPSLTTPAGIYTANLNMIATGTF"
     gene            complement(7732..8475)
                     /locus_tag="VN778_01080"
     CDS             complement(7732..8475)
                     /locus_tag="VN778_01080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49604.1"
                     /translation="MKKLLRILLISAPLVLGLGAIAHATCSPDFQTCSSGYGVNEVFM
                     GSGGELNACSTNFCSKQSAGETAVGNTSSPSYQAQAGFNTDRTPYIQFNVNTTNINLG
                     TITSTATQTATATFSVKAYLSHGYAVVNASDPPTNGAYTMQTMTTQGPSVVGTEQFGI
                     NLAANTSPTTFGADPVQVPSPSFATGFATTEYSLANNYKYVKGDTIAESAASTSETDY
                     TISYIFNISNVTPGGTYNFHHVLVATATY"
BASE COUNT         1989 a         2296 c         2249 g         2004 t
ORIGIN      
        1 acgagctaga agcccttgcc tgccccagtt caggtgctat ttcactcaaa taggctagtt
       61 tacccgcctc gctgtgcatc ggctcccggc cattcgcacc attgctaccg ctattatgtt
      121 gcggccccca cccctctaca ataaattggc cgagagaacg tgcatcttct atgaggctga
      181 actctcgtat ctcgcaacca tacaaaagca gtttgccggc acacttctct atcatccgct
      241 tttgtaatac cgcgtcttcg tcaagaaggt ttcctttttg gtcaaattca ccttctacta
      301 ccggctggcc atgcgggtcg cgcaggactt cgcccatgac gcttcggaaa gcaccggtaa
      361 aaataccatc ggcaatgtcc tttaaaggct cggtgctcgg ctcacccttt ttcacatcgg
      421 gcctggccgg tttaaaaatt gcccggaagg gatgaggtgc ttctggttta acgccccaga
      481 taacctgggc accttcatta cgttgcttac cgtcgctgca gtctaccggt gtagaaatga
      541 ttgagcggcg gttcattata ttcactaata agatattgcg agtgaagggt aaatggcctc
      601 gcagcccagg ctgggcaatt ccgtgcaaaa atttttctac ttcagtgtga tttaggggaa
      661 ccatgctcgg ttggccgcgt ctcaggcgca ctccccgata gtcagcatct ataatttgaa
      721 caatccctgt tttggaagcg actttgctgg cgccgccaag agtaccggca cctgcaatca
      781 ggggccacat gtgtcctact acagatgata tctctgatga agcagccatt tcgttctctt
      841 taattcctca cgtattaact atgggtctta ttacctgacc ctgactcaaa acttcttgcg
      901 gaagactcat cgttagcctc aatatgcctt atttgaagaa aaattacaag gctacattta
      961 atttacaaca ccgacaacgt tactggctta acctccgcca ccaaggcctg atgatccccg
     1021 acaggaaccg gcattacttc tactccataa gcacgcaagc cgtgtactgc gatgtggtcg
     1081 atctgctgat cgtactcatc ggctccttcc gacttgctgg tcgggccgcc ttcgagcact
     1141 gtaaaagcaa atggttttag ggtttcgtgg gcgtatggca agcgaacatt gtaatctccc
     1201 agtaatatat gcgggcctgg ccgaaacagc agggccgtag ctgcatgctc gagcatttct
     1261 cggcgccgcc ggccaccagc atgaatcacg ccgacggcta ggtcgtagcc attgacgcag
     1321 acatcaccaa tgagaacatt gcgcggttca cgtatcactg ggattttgtg atttccgact
     1381 gtgattcttt gccaatcacc agtgagcttc aggctgtcgt agcttttgat ttcacctcgc
     1441 accagcaagg cattaccaac tttgcctctc gccctaaatg tgcgcgtcgg tgcgaaacgg
     1501 taatccatac cacaagcttt cgcagcatag gctgccaaat tttctttgcg cgagcgcggc
     1561 acgcccgcat caacttcttg aagccccaag acgtccgcat ttatttgacg gcaagcttcg
     1621 gccaccagct ccggatttcc tgggtcgctg taactgcctc tcgaaccatg ctgaatattg
     1681 agcgttgcca ccctgatcaa agccgtctct atcatgtggg ttacagcgta gcagattgac
     1741 aaatagcgcg ccaacagtaa gcttaatata gatgaaaaca ctggaaaacg ctttcagtct
     1801 cgatctgtta aaacaacgca ttcatagcct gaagctcgac ggttttgttg tctatacaac
     1861 cgttgatttt ccgcttaaag gggaggcgac ttatggagca ctcgtcatgt ctgtcgatga
     1921 tcacgcctcc atcttacagt tcgtcaatga attacatgac gatcagtctt tgggaccgca
     1981 tgagtattta tctgatgaca tcattcgtga agaccggcgt tacgtcgaaa ccgactccgc
     2041 ctatgccgca ctgaccaact cggaagaact atgcagcaaa ctcttagaca acgcgctacc
     2101 agacgagtac attagcctag acaatacgtc ctggtatctc taagcgcctg gttacgaaat
     2161 aattccgccg cccagaacgc aaccgtcttt atatagcaca gccgattgac cggcagctag
     2221 accccgttgt tcatctttca aaaagagtat ggtgcctttt acagtgcact cgacaagggg
     2281 tccgctgtag cgcaaacgca cttggtaggg cttatcttcg gcaggtggct ggtttatcca
     2341 gtgcagatcc tgtaaatgaa cctctttgtg ccacagccct ctatcatcaa gatttgtagt
     2401 tacataaatg gtattcgcct tcatatcttt gcggctgacg tagtatggca agccgccgcc
     2461 aacattgagt ccgcgccgct gcccgatggt ataaaagatg gcgccgtcat gctcgccgat
     2521 aaccccgcct ttttggtcga tgatcggacc acgcggctga ggcccaagct cgttcagcaa
     2581 gaactccttg atgccaactt tgccaacgaa gcagatacct tgactgtcct ttttgtcggc
     2641 ggtggccagt cccagctttt tagccagcct gcggacttcg gttttgtgga gttcgccgat
     2701 tggcatcagg gacttttgca gagccgcttc ggtcactcga tagaggaaat acgattggtc
     2761 cttagcgggg tcgagtccgg cgaaaagttg tccatcttta atgcgcgcat agtgaccagt
     2821 agcaatcata tcagcgcctt cggccagcgc cgcttccaga aaaagcttga atttgacctc
     2881 ttgattgcac ataacatcag ggttcggcgt gcggccggcc tggtactcgc gcaccatgta
     2941 gtcgacaact ttttgccggt attctgtctc gaaatcgaac attttgaagt cgatcccaag
     3001 ctgtaccgct acccgcttgg catcctgata atcttctttc caggggcaaa caaagccggg
     3061 caagtcctgg ctccaatttt tcatatacac accggtgacg tcgtaaccct gctcctgcaa
     3121 gagggccgcc gtgaccgacg aatcaacacc gccggacatc ccgacgtaaa ctttctttgc
     3181 catatctata ctattttatc aaatatccac ggtttaggcg cgctcttcgt cgcggctggc
     3241 ttccaagcgt ttacgtaagc tgtagagttc gtggcggcgg ccgatccaga aagctgcaaa
     3301 gatcaggggg aacatggcga gggccggcca ccagagcacg tcatgctcga tgacggcgtt
     3361 gccgctcccg cccttagcgt tattctggat ggcgcccgtg gcttggccca ccaactgcag
     3421 gaaagctgta ccgccgtttt tatcggtggc tttgacaatc accttgtaga taccggcggt
     3481 tttgtaggtg tgtttgatcg tgatggttcc ggcggaggcg acgctcatca ggtctgtcgg
     3541 cgaaccatcg ccccagtcga cactaacagc gtaggggccc gtgccgccac tgagcaagat
     3601 cggccaatca atctcttggc tgggaggggc gccgcgttca gcgtaagcgc tgctcaattc
     3661 aacatgggta ccaaactgca ggaactgcgc atcattgaaa gtcaccgtca cggtgttcga
     3721 gtccggcccc gcttgatcga gcgagtcata gacgcgcgcc acgaggtcgt tctgaccgct
     3781 aaaaagatcg atttgcaccg aatagctgcc gctggcgcaa tacgtcgcgc cgacaaagac
     3841 gttattagag aaaatcttca ccagcagtcc ggtcgggcac agaccgctga cggtaatcgg
     3901 gacattggtg aagacggcac cgttccccgg cacagcaatc gttgcgcctc gggtcggcgc
     3961 agcagacgaa atctcgccct gcaaaccaat cgagccggac gaggattgcg gtggaaaggg
     4021 tgaagccaaa ctatgcgagc caaatgccaa gaaggacagg cctaaaacca gcaaccactg
     4081 agtccgtctc ttcatgtata taagcataac ctaaaaggct atagagttgg tagcgttagc
     4141 ggcgaccagc ccggcgcaaa atacgccggt tgtaagcccg gatgagccgc ggcaaaccga
     4201 agaccaggaa cagcagcagc aaaacgagca gcacgaagcc gataatcact gagataggaa
     4261 tgacataaaa cgtcgtgctg gcgctgagca attggccgcc cgagccatag ccgaaattac
     4321 cttcgacttt gtattggccg aacgagccga ccttgttgag ggcaatgtta aagcgacgga
     4381 tgctatcggg cagcacgttc cccggcggat aggtgttatt cagctcatag gtagctagga
     4441 tcttgccgct atggttttta agcaagattt taccgaacgg ttcttcctgg acgttgcctt
     4501 cgttctggaa gcgcaccgcc gccgtaatat ccttattact ggtaaagaag ctgctggcat
     4561 cacccttctt gtcgcgcacg tcgaaactgg ctaggctgag cttgtcggta atgttccccg
     4621 gcacctttac caggattagg gagccgacgc tgccggccag agtcacattt tgattgggag
     4681 cggcggagac gttggccggg gcaaaacgaa cagcgccgaa gtagccgccg ccgggcgcat
     4741 tagcgggaat cgcgaccgtt accggcacgg atttttgctg gccggctgct aaggtaaaat
     4801 ttttgattgg ctgaacataa tgctttaagc cgtggctggg agcggactgg ttcgggtcaa
     4861 gaatgatggc cggattaccg ctttcgtcag ggttggctac gaagtcgttg acaatagcct
     4921 ggaaggtagc agagacggtg gtcacattcg tcacattgat attaattgtc tgtgacttgc
     4981 ctggattgat agtcaggtcg ctgcgaaccg gcgaaacacg aagaccatta ccacccgcag
     5041 cagtagcagt cggcatcaag ccacccatga ccaccagcaa ggttgtccct atactcgcca
     5101 gcgttaaccg gatatttcgg cgtacgtgtg tgtttctcat ctctttaatt cctataatat
     5161 agcataaact gtatggcctt gcacctagta gccattaaaa ggtggccaga gcgatgtagg
     5221 tcagcgtcga gacatagatg cccgatgcct ggcctttggg cacgttgacg atgtagctgg
     5281 cggtgaattc acggtaattg tcggtcgtgg ggttcgagac caaggtatca cccggattaa
     5341 actggtaaaa attaggctga ttgtaattag ccaccggaac gccagtgccg gggccggtgg
     5401 gattaatgcc gacagccggc gtggaattag cccgcaggtt catgccgaac tggtcggtcc
     5461 ccgggcgaga aacgtcatta gctgtcagag caccgatgac attgacaccg ctcgtcatag
     5521 tcgtgccgtc aaccgtgatg ttatagccgt tgccggcatt ggtggccacc agcatttgcg
     5581 acgaaccgct gcgcgccgag ctggaagaga gctcgccgaa atcaatgaag tcaccttcgg
     5641 cgttagcgca gttcaggcca ctaatggtaa cgccagtgca aaatataaga tacggcggca
     5701 cctcagcatt cagctgtacc ggaccattca cgatcgcgta agcaataccg ccctcatctg
     5761 tttccggacc cgtggcatcc gccgtcgcat acgtctgcac ccgcacaaag tacgaaccgg
     5821 acgcattagg gttagtaaca cccgtgaaat gatattcgac atgtccagcc gtggcagcag
     5881 ccggcgcccg cgtcaaaatt atctcatttg gcgtcgaggc accactgatc gtgaaaccag
     5941 tctgccctgt ctggctggcc aacaccgccg cactatcatc aaaaccgctc gaggccgtac
     6001 aaggatcccc cggcaaggca ctattagaac aaaactgcac cacaatcgac cccagcgtcc
     6061 caggcgtagt caaattaaac gtcaagagat aatcgctcgt cttactcacc tcattatcac
     6121 tcagctgcat agcccgttgc ccaatctgcg ccccagccac cggcaccgtc agatagtgcc
     6181 cgacacctac caacacaaac atagccacaa ccagcccaac aagaaatcgc cgagatttct
     6241 gagtactccg agacataggg gagaaccccc tagaaggtgc ccgtggcgat catgttgagg
     6301 ttagcggtgt aaataccagc tggagtcgtc agtgaaggcg tcgccgcaaa gacataatgg
     6361 ttgtcagcat ggtgaaccgg cgtggttgta gcggcaacga tgctaccgaa ggtggtctcg
     6421 acgttaccga cattggaggc gggcgcacca ccattagcag ctgccgtggt agtgtccata
     6481 ccataccatg gggtagcatc ggtgcaggaa ttgcctgagt ggaagtacgg cgcttgcggt
     6541 gtcatgctgc ccacgattgt actatcaacg ggcgaagaag gcgtatatgt caccgaacac
     6601 ataccaaatg ctgctgtacc cgcagcaatg ggggcgccag tagagtttcc actattcact
     6661 gcaggaatgg cgcacgtcgt gccactatca gcgctcaggc cgccacaaag caaattgcta
     6721 tttcgcatat caattaccgc accatgcgta gcgttcgttg atacctgcga atatatactg
     6781 ccagtatcaa ctcgcgacgg gtccaagacc ggctgcccag cacctactgc aacaccaagg
     6841 gtaacaacag gcgggttttg acccacttgc tgatcagcac agtcatcatt actaggcgtc
     6901 gcccatgtag cagggtcagc gtcactgaca caaaatgtta actgttcctg gacgcgggcc
     6961 gtaatctgaa tggcactggc tgtactgagg gcaatgccgc cgtagtcttc atcgcctccg
     7021 acattggtag cgctactata agtgccccag cttgttccgc tataggtata aatgcgggca
     7081 tagaaggtgc acgccaaggc cggactcgta cagttagaag aactcgggtt agtaataccg
     7141 gtaaggtcga actgttgcgc accggctgtc attgccgagc cagaaccttg tgccaatttc
     7201 acctgagacg ctccagctgt tactgtccac gccccaccac cttgtacgtt accaccgctt
     7261 gcaacggtcg agatagccgc actcgccgcg ttcatacccg tcggcccagt acaggtatcg
     7321 ccgataattg gatcttcttt acagaaatca attaccaagc ttgcggcagc ttgcgtagtc
     7381 gtaaaactga ctcgatacgt cacgctggta ccgctaccca cgccgctagt aatggatgcg
     7441 ttgccgctcg gcgccgaatc actcatctgt atactgcggc tctggagctg gttagggttg
     7501 gctgccgctt tacccataaa tatagcaggg gctgcagaca cgaacagcaa ggcagccagc
     7561 atggcgcaga tgactctgcg ggccatgcga acgtcgaacg aaaacaggtt attcatatga
     7621 ggtagtaatt tttgtttaaa ctatacgcag taatcgtatc acgcttgtgg taacctagca
     7681 agtattttgc ttctcctaat aataagtagt tatccacacc cagcactatc actaatacgt
     7741 cgcggtagcc actaagacgt ggtggaaatt gtaggtgccg ccgggggtta cattgctgat
     7801 gttgaatata taagagatgg tgtagtctgt ttcgctggtg ctggcggctg attcggcgat
     7861 tgtatcgcct tttacgtatt tgtagttgtt cgccaagctg tattccgtcg tggcaaagcc
     7921 ggttgcgaag ctgggacttg gtacctgaac cggatctgcc ccgaaggtgg tagggctggt
     7981 gttggctgct agatttatgc cgaactgctc ggtgcctact acggaaggtc cttgggtcgt
     8041 catggtttgc atggtgtagg cgccgttggt gggtgggtcg gaagcgttta ctactgcgta
     8101 gccatgagag agataggctt taactgagaa ggttgcggtg gcagtctggg tcgccgtaga
     8161 ggttatggtg ccgaggttga tgttggtggt gttcacgttg aactgaatgt acggggtgcg
     8221 gtcggtgttg aagccggctt gagcttggta gctggggctg gaggtgttgc caacggcggt
     8281 ttcgccggcg gattgtttgg agcagaagtt ggttgagcag gcgttgagct cgccgccgct
     8341 gcccatgaag acttcgttga cgccgtagcc tgaggagcag gtctggaagt cggggctgca
     8401 ggtggcatgg gcaatcgctc ccaggccaag cactaagggc gcgctgatca gcaatatccg
     8461 taatagtttt ttcatttctc tgttaccaca agcactatac gtatataata acagtatggc
     8521 cgaggaaaat caggcgcc
//
LOCUS       DATNGP010000043         2648 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_3666529, whole genome shotgun sequence.
ACCESSION   DATNGP010000043 DATNGP010000000
VERSION     DATNGP010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2648
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3666529"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            75..1727
                     /gene="argS"
                     /locus_tag="VN778_01085"
     CDS             75..1727
                     /gene="argS"
                     /locus_tag="VN778_01085"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HXR49605.1"
                     /translation="MKSELEAKVAAAVQQVFGVEVTVELTRPEEQFGDYATNVALQLA
                     AKLQKNPQEVAETLAGHLRESLAEQISEVSIAGPGFINLILTNQALQGAARQEPQKSY
                     EGQEILVEFGDPNPFKEMHLGHLYTAIVGDAIARLFEASGGTVQRLSYHGDVGMHVAK
                     AIWAIRKRLNETANLQIADCFAGDTVVGHYYAEGARAFDEDPAAEEEIRQINASIYAR
                     DDEQINAIYDLGRDLSFRNFDQIFEELGVHFQKRYLESESTKAGLQFVQANLGSVFEE
                     SEGAVVYRGEKAGLHTRVFINSRGLPTYEAKDLGLSELKNQDYPQANKSVIITAHEQS
                     EYFKVMLAALAEIDPPLAAKTQHLAHGFLSLTTGKMSSRTGQVFAAVNLLDDVRAAAT
                     EQFPESEVQQEVYLAAVKYTFLKNRVGGDIIFDVQESVALEGNSGPYLQYAHARARSI
                     LRKAERQGALDGAELEPEERTLVRKISEYPEVVDKAVNELMPHHICTYLYELAQTFNR
                     FYEHNRVLGDPREQERLGLVSEYADTLRKGLMLLGIASPEKM"
     gene            1761..2228
                     /locus_tag="VN778_01090"
     CDS             1761..2228
                     /locus_tag="VN778_01090"
                     /inference="COORDINATES: protein motif:HMM:NF013869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MscL family protein"
                     /protein_id="HXR49606.1"
                     /translation="MAERKRKRQTKKVGTTTLVTTGSTIRIEQPKSRRQPKPKVAVVV
                     AQEINPVSGFTDFLREHAIVGLSIGFIMGSQMATVVKVLVTNFIDPLSRLFLGTALSQ
                     RTFTLRFHDRVANFGWGGVVSALIDLILLLIFIYLAFKFFNLDKLDKPQDPAR"
     gene            2270..>2648
                     /gene="msrB"
                     /locus_tag="VN778_01095"
     CDS             2270..>2648
                     /gene="msrB"
                     /locus_tag="VN778_01095"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007919560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HXR49607.1"
                     /translation="MSDDEWKDKLTPEQYHILREKGTEAAFTGKLLHNDKTGNYTCAA
                     CGKVVFTSDTKFDSGSGWPSFYDVANTKAVELHDDAGHGMHRVEVMCANCGSHLGHVF
                     NDAPDQPTGMRFCINSAALDFKEK"
BASE COUNT          684 a          681 c          706 g          577 t
ORIGIN      
        1 ttgggtaggc taaaaatagt tccaacttca tctgccacgg tccaccagta gtcctactgc
       61 tataattcat aggtatgaaa tcagagcttg aagctaaggt tgctgcggcg gtgcagcaag
      121 ttttcggcgt agaagttacc gtggagctga cgcggcccga agagcaattc ggcgattatg
      181 ctaccaatgt ggccctgcag ttggccgcca agttgcagaa aaatccgcag gaggttgccg
      241 aaacgctggc gggtcattta cgtgaaagct tagctgaaca aattagtgag gtatcgatag
      301 ccggtccggg atttatcaac ctcatattga ccaatcaagc cctgcagggc gctgcgcggc
      361 aagaaccgca gaaatcctac gaaggccaag aaatcctggt cgagttcggc gacccgaacc
      421 ccttcaagga aatgcacctc ggacacctct ataccgctat tgtcggggat gctattgcac
      481 gattgttcga agcaagcggt ggcaccgtgc agcggctaag ctatcacggc gacgtcggca
      541 tgcatgtcgc caaagccatc tgggctatcc gcaagcgact caatgaaacg gccaaccttc
      601 aaatagccga ttgtttcgcc ggtgacacag tcgttgggca ttactatgca gaaggagcta
      661 gggcttttga cgaggatcca gccgccgaag aagaaatccg ccaaatcaat gccagcattt
      721 acgcccgtga cgacgaacaa atcaacgcca tttacgacct tggacgcgac ttaagtttta
      781 gaaatttcga tcaaattttc gaggagctgg gagtgcattt ccagaaacgt tacctggaaa
      841 gcgagtcgac caaagccggg cttcaatttg ttcaagctaa tctcggcagc gtgttcgagg
      901 agagcgaggg agcggtcgtt tacagaggtg aaaaagctgg tttgcatact cgggtattca
      961 taaactcacg cggcttgccg acgtacgaag ccaaagacct cggcctgtcg gagctaaaaa
     1021 accaggacta tccgcaagcg aataagtccg tcatcatcac cgctcatgag cagagcgagt
     1081 acttcaaagt catgctcgcg gccttggccg aaatcgaccc gcctttagcc gctaaaacgc
     1141 agcacctagc gcacggcttc ttgagcctca ccaccggaaa aatgagcagc cggaccggtc
     1201 aagttttcgc cgccgtaaac ctactcgacg acgtccgtgc cgctgccacc gaacaatttc
     1261 ctgaatctga ggttcagcaa gaagtgtacc tcgcggctgt gaagtacaca tttttgaaga
     1321 atcgtgtcgg tggcgacatc atctttgatg tgcaagaatc ggtggcgttg gagggaaata
     1381 gtgggccata tctgcaatat gcacacgcta gagctcgatc gattttgcga aaggccgaaa
     1441 ggcaaggggc tttggatggt gccgaactcg agccggagga gcgaacgctg gtgcgcaaaa
     1501 tctctgaata cccagaagta gtcgacaaag ccgtcaatga gctcatgccg catcacatct
     1561 gcacgtatct ctatgaacta gcgcagacct tcaaccgttt ctatgagcat aaccgagtgc
     1621 ttggcgatcc gcgcgagcaa gagcgcctag ggttggtgag cgagtatgcc gatacgctcc
     1681 gcaaaggtct tatgcttttg ggcattgcat ctccggaaaa aatgtgatat aatgtaacag
     1741 ttacatcaaa aacaaaaatt atggcagaac gaaaacgaaa acgccaaaca aaaaaagttg
     1801 gcaccacgac gctggtcaca accggcagta ccatccgcat cgagcagcct aaaagccgcc
     1861 gccagccgaa gcccaaagtg gccgtggtgg ttgcacaaga aatcaacccc gttagcgggt
     1921 tcaccgactt cttgcgcgaa catgccatcg tcgggttatc catcggtttt atcatgggta
     1981 gtcagatggc cacggttgtc aaagttctcg taactaactt catcgaccca ctttcgcgtc
     2041 tgtttttggg taccgcgctc agtcagcgaa cgttcacact gcgtttccac gaccgcgtag
     2101 ccaacttcgg ttggggcggc gtggtgtcgg cgctgatcga ccttatcctg ctcctcatct
     2161 ttatttatct cgccttcaag tttttcaacc tcgacaagct cgacaaacct caagatccgg
     2221 ctaggtaatt tccgcagaca cggtttaatg accgcggtat aatgctcgta tgagtgatga
     2281 tgagtggaag gataagctga cgcctgagca gtaccatatc ctgagagaga aggggaccga
     2341 ggcggcgttt actggcaagc ttttgcataa tgacaagacc ggcaactata cctgtgcggc
     2401 ttgcggcaag gttgttttta ccagtgatac gaaattcgac agcggttcgg gctggccgag
     2461 cttttacgac gtggcgaata ccaaagccgt cgagctgcat gatgatgccg gtcacggtat
     2521 gcatcgagta gaagtgatgt gcgctaactg cggcagccac ctggggcatg tgttcaatga
     2581 tgcgccggac cagccgaccg gcatgcgctt ttgcatcaac tcagcggccc tcgattttaa
     2641 agaaaagg
//
LOCUS       DATNGP010000044         4692 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_2555020, whole genome shotgun sequence.
ACCESSION   DATNGP010000044 DATNGP010000000
VERSION     DATNGP010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4692
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2555020"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            211..561
                     /locus_tag="VN778_01100"
     CDS             211..561
                     /locus_tag="VN778_01100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49608.1"
                     /translation="MAVGKTLLYLIYAVIVAGMIVAIVLSFHTSKTPAPKTQSSASSH
                     QQVSRGQATQPKTKPTPATSSTSTTTPSTSQSGSATSPRLTNTGPGDTALIFLAVSGL
                     SALAYRRYQLQRKI"
     gene            636..2057
                     /gene="pyk"
                     /locus_tag="VN778_01105"
     CDS             636..2057
                     /gene="pyk"
                     /locus_tag="VN778_01105"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HXR49609.1"
                     /translation="MDTIESSFSTYKHAKIIATIGPATDSYEAIFSLIKAGADGLRLN
                     FSHGTHEERTRQIRWIRRASKALNKPVAIIQDLQGPKIRLGDFDGIVNVRQGQALSFV
                     YQADYNVSGHLPTQYDLGHKVKRGERLFLFDGKIRATVTSVREGVVRATAENEGILVR
                     RKGMNLPDTDFGGDIITAKDKADLVYGSAQDIDYVAMSFVQSAEDIENLQKLLRSMNS
                     DAKIIAKIETKAAVSNIESIVQASDVVMIARGDLAVETPAESVPIVQRQIIGLGQRYA
                     TPTIVATQMLGSMTEAAEPSRAEVSDIATAVLIGADCVMLSDETASGKYPLEAVEIMQ
                     RVICYTENHSPLTVSFPGNEEHSRQVAISAAILSLADNIAAKAIIAETKSGATALQIA
                     ARRSTTPLIAVTSDARTAQQLAIVYSVKSYVRPVDPLAAVKLTDWLHKNKVLHPGDIV
                     VTASGRYPGVVGTTDTIKVRVLE"
     gene            2119..3405
                     /locus_tag="VN778_01110"
     CDS             2119..3405
                     /locus_tag="VN778_01110"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HXR49610.1"
                     /translation="MFTKQTIRDIDLAGKTVLLRADYNVPLENGKITDDYRIKQSLPT
                     IHYLLEHNVKLIICSHLGRPKGPKDLENSLFPVAKRLKQLLDRHVAFATDCVGEPAHK
                     AAEGLKRGEVLLLENLRFHPEEEANDDNFARELASLAEIFVQDGFGVVHRAHASTSAI
                     THHLPSVAGLLLEKEVDTITRVMESPERPLVAIVGGAKIADKLEILHRFIEIADFVAV
                     GGAMANTFLAARGIDVAESLYDKADLPLARDIMRKAGGEARKRRFIFAIPHDGVVAHK
                     IDKSAPTRIVDWTSHVIADIENYPRRVPNSASHVHAHERILDIGPFSGSFIAGGIQLA
                     NTVIWNGTMGVTETESLQGPIGPYSHGTELIIEALLGEFGHKPFTVVGGGDTVGYVEE
                     RKLVSAFGHVSTGGGASLELMSGRKLPGVEALLNKE"
     gene            3427..4212
                     /gene="tpiA"
                     /locus_tag="VN778_01115"
     CDS             3427..4212
                     /gene="tpiA"
                     /locus_tag="VN778_01115"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HXR49611.1"
                     /translation="MKRSLIVGNWKMHLDVQAASMLVHRLDKAIKPHREIEVVLAPSL
                     LALQPVSREIDRRKFRLAAQNAFYKDEGAYTGEVSFTMLRELVHYAIVGHSERRIYFN
                     DTLEIVRDKVQAAIRNGITPILCIGETKQERSLGETRRVVHDQLTTALANLTTEEVEQ
                     LVIAYEPVWAISTFDGETAKPDDIGKVFELIRAEISELYGQAAAENVRLLYGGSVNAD
                     TVGGYLAIPGCNGTLVGAASLNYQQFSAIVDTAYRLLHTDEQA"
     gene            4209..>4692
                     /locus_tag="VN778_01120"
     CDS             4209..>4692
                     /locus_tag="VN778_01120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49612.1"
                     /translation="MSDIKKPSKAAAPSKRIMDVAHPGKSAPSSTSKPVIVTNRPIMK
                     DPMMTEDSDVNTGNEVKIVGTSAKIKIQPLTAPVVEAADPEQETIEVKTSSKPMPEPP
                     PPAPEAAEEPEPAEKEVDTPKPEPETPPAEASKPADTKDDQPEADDKVSEKADAAESE
                     D"
BASE COUNT         1204 a         1210 c         1256 g         1022 t
ORIGIN      
        1 aagcggtaga aagggagcaa taatcacgta agcattgagt aagatgatga gcgctaggag
       61 tatgttgttt atctttgaga gcttcatcac tagccatcat acacgcttta cgtagtttat
      121 ttgtcagcgg agccataagc acgcagaata actacttgca aaaaatccta attcagttta
      181 ctgttaagac attaagtgga ggaacttaat atggccgtag gcaaaacact gctgtatctg
      241 atttatgcgg taattgtggc cggcatgatc gtggccattg tcctgtcatt ccacacctct
      301 aaaactccgg cgccaaagac tcaatcgagt gcgagtagtc accagcaagt ttcgcgtggc
      361 caagccactc agccgaaaac taagccaact cctgcgacgt cttctacgag taccacaacg
      421 cccagcacta gtcagagcgg ttcagccacc tccccacgcc ttaccaatac cggcccggga
      481 gacacggcgc tcatttttct ggccgtaagc ggtttgagcg ccctagccta tcggcgctac
      541 cagctacaga gaaaaatata aaacgtgcgg ctttggtttt gtaatactgc agccaaagcc
      601 gtatactaaa cataagcaat aaattcttga aatgggtgga taccatagaa tcatcatttt
      661 cgacgtataa acatgccaaa ataattgcca ctatcggacc ggcgaccgat agctatgaag
      721 cgattttctc acttatcaag gccggagccg acggtttacg cttgaacttt agccatggca
      781 cacacgagga gcgcacgagg caaatcagat ggatcaggcg ggcgtctaaa gcgctgaaca
      841 agccggtggc gattattcag gatttacaag ggccaaaaat ccggcttggc gactttgacg
      901 gcattgtaaa cgtacggcag ggccaggctc tgagttttgt ctaccaggct gactacaacg
      961 ttagcggcca tttaccaacc cagtatgatc tgggccacaa agtaaaacgc ggtgagcggc
     1021 tctttttgtt cgacggtaaa attcgtgcca cggtcaccag tgtgcgagaa ggtgtggtgc
     1081 gggccacggc agagaatgaa ggtattctgg tgcgccgcaa aggcatgaat ctgccggaca
     1141 ctgattttgg cggggatatt attactgcca aggataaagc cgacctcgtc tacggatcag
     1201 cccaggatat cgactatgtg gcaatgagct tcgtgcagtc cgcagaggac atcgagaact
     1261 tgcaaaaact tctccgtagc atgaatagcg atgccaaaat tatcgccaaa atcgagacta
     1321 aagccgccgt cagcaatatc gaatcgattg tccaggctag cgatgtggta atgatcgctc
     1381 ggggcgatct ggcagttgag acgccggcgg agagcgtacc gatcgtccaa cggcaaatta
     1441 tcggccttgg ccagcggtac gccacgccga ccattgttgc gacgcagatg ctgggcagca
     1501 tgaccgaagc cgccgagcca tcgcgcgccg aagtatccga catcgccacc gccgtgctta
     1561 tcggtgccga ctgcgtgatg ctgagtgatg aaacggccag cggcaaatat cccttagaag
     1621 ctgtcgagat aatgcaacgg gtgatttgct acaccgaaaa tcactcaccg ctgacggtta
     1681 gctttcccgg gaatgaagag catagccggc aagtcgccat cagcgcggcc atcctcagct
     1741 tagctgacaa tattgccgct aaagctatta tcgccgagac caaatccggc gccactgcgc
     1801 tgcagattgc tgcccgacgc tcgactacgc cgctgattgc tgtcaccagt gatgctcgca
     1861 cagcccagca actggctatt gtgtatagcg tgaaaagtta tgtgcgaccg gtcgatccac
     1921 tagccgccgt taaattaact gattggctgc acaaaaataa agttttgcat cctggcgata
     1981 tcgtggtgac tgcctctggc cgttatccag gggtcgtcgg cacaaccgat accatcaaag
     2041 ttcgcgtgct ggagtagggg tttgtggatc gtcggttgta gtttatagta gacagtaatc
     2101 aaaggtgggc ctcttacagt gtttactaaa caaacaattc gcgatatcga tttagctggt
     2161 aaaaccgtgc tgctcagggc tgactacaat gtgccgcttg aaaatggcaa aattactgat
     2221 gattatcgta tcaagcagtc gttgccgacc attcactacc tgcttgagca taacgtcaag
     2281 ctaatcattt gctcgcatct ggggcgtccg aaggggccca aagacttaga aaacagccta
     2341 tttccggtag cgaagcgcct caagcaactc cttgatcgtc atgtagcgtt tgcaactgat
     2401 tgcgtaggcg agccggctca taaagccgcc gaagggctga agcgtggcga agtactgctc
     2461 ctggaaaatc tgcgctttca tccggaagag gaggccaatg atgataattt tgcacgggag
     2521 ctagcgagct tagctgagat atttgtccag gacggttttg gggttgtgca ccgggctcac
     2581 gccagtacct cagcgatcac gcatcatttg ccgagtgtgg ctgggctact gctggaaaaa
     2641 gaagtcgaca ccattactcg cgttatggag tcgccggagc ggccgctggt ggctatcgtg
     2701 ggcggcgcca aaatagcaga caaacttgag atactgcacc gcttcattga aattgccgat
     2761 ttcgtggctg tcggtggtgc catggccaac acgtttttgg cagcccgcgg tatcgacgta
     2821 gccgagagcc tctacgacaa agccgatttg cccctcgccc gtgacattat gcgtaaagcc
     2881 ggtggcgaag ctcgcaaacg ccgctttatc tttgccatac cccatgacgg cgtggttgcg
     2941 cataagattg acaagtctgc accgaccaga atcgtcgatt ggaccagtca tgtcattgct
     3001 gacatcgaga attatccacg ccgcgtgccg aacagtgcca gtcatgttca tgcccatgag
     3061 cgaatacttg atatcggccc cttcagcgga tcatttattg ccggtggcat ccagctggct
     3121 aacaccgtca tctggaatgg caccatgggt gttaccgaga cggaaagttt acagggcccg
     3181 atcggtccgt attcgcatgg taccgagctg atcatcgagg cgctgctggg agagtttggc
     3241 cataaaccct tcactgtggt cggtggaggc gacaccgtgg gttatgtgga agagcgcaaa
     3301 ctggtaagcg cctttgggca cgtgtcgacg ggcggcggcg ccagcctgga actgatgagc
     3361 ggccgcaaat tgccgggagt cgaagcgcta cttaataagg aataaggtac aatagccata
     3421 agcattatga aacgctccct cattgtcggt aactggaaaa tgcatcttga tgtgcaggct
     3481 gctagtatgc tggtccatcg tttagacaag gccattaagc cgcaccgcga aatcgaggtt
     3541 gttctagcgc caagcttgct cgccttgcag ccggtgagcc gcgaaatcga tcgccgcaaa
     3601 ttccgcttgg ctgctcaaaa tgccttttat aaagatgagg gagcttatac cggtgaggtc
     3661 agcttcacca tgctgcgtga gctcgtccac tacgcgattg tcggccactc cgagcgccga
     3721 atttacttta acgacacctt ggaaatcgtc cgcgataaag tccaagcggc tatccggaac
     3781 ggcatcacgc ccatcttatg tatcggcgag actaaacagg agcgctcgct tggcgaaacg
     3841 cgccgggttg tacatgatca gttgactaca gccttggcta atctgacaac tgaagaagtg
     3901 gagcagctag tgatcgctta cgagccggtg tgggccatca gtactttcga cggagaaaca
     3961 gcaaagcctg atgacattgg caaggtgttt gagttgatcc gggctgaaat cagtgaactg
     4021 tacggtcaag ccgcagccga aaatgtgcgc ttgctatacg gcggtagtgt caatgccgac
     4081 accgtcgggg gctacctggc catcccaggt tgcaacggta cgctggtcgg cgctgccagt
     4141 ttgaactatc agcaattttc ggccattgtc gatacggctt atcgcttact gcacacggat
     4201 gaacaagcat gagtgacatc aaaaaaccct ctaaggcggc tgctccctca aagcgtatta
     4261 tggatgtggc tcaccccggt aaatctgcgc catcgtcgac gtcgaaaccc gtgatagtca
     4321 ctaatcggcc tatcatgaaa gatccgatga tgacagagga ttcagacgtt aatacgggta
     4381 acgaagtcaa aattgtcggc acttcagcca agatcaaaat tcaaccgctc acggcgccag
     4441 tcgtcgaagc agctgacccc gaacaggaga ccatagaagt taaaacctca agcaagccaa
     4501 tgcctgagcc accgcccccc gcgcctgaag cagccgaaga gcccgagccg gcagagaaag
     4561 aagtagacac accaaaacca gaacccgaaa ctccgcccgc agaagcaagt aagccggccg
     4621 atacaaaaga tgatcaaccg gaagccgacg ataaggtcag cgagaaggct gatgcagctg
     4681 agtcggagga tt
//
LOCUS       DATNGP010000045         2349 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_3104383, whole genome shotgun sequence.
ACCESSION   DATNGP010000045 DATNGP010000000
VERSION     DATNGP010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2349
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3104383"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..315)
                     /locus_tag="VN778_01125"
                     /pseudo
     CDS             complement(139..315)
                     /locus_tag="VN778_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611077.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            406..1316
                     /locus_tag="VN778_01130"
                     /pseudo
     CDS             406..1316
                     /locus_tag="VN778_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611730.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(1352..1576)
                     /locus_tag="VN778_01135"
                     /pseudo
     CDS             complement(1352..1576)
                     /locus_tag="VN778_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611153.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            complement(1610..1888)
                     /locus_tag="VN778_01140"
                     /pseudo
     CDS             complement(1610..1888)
                     /locus_tag="VN778_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019054811.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            1898..2086
                     /locus_tag="VN778_01145"
     CDS             1898..2086
                     /locus_tag="VN778_01145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49613.1"
                     /translation="MANQNVSTASLLSLVYIVLPEDQTLIMEKNCTMLIVRYIIQIPE
                     KKGFLVLTDTTFHLSYPI"
     gene            2152..>2349
                     /locus_tag="VN778_01150"
     CDS             2152..>2349
                     /locus_tag="VN778_01150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49614.1"
                     /translation="MDDQGQKIEYEVYFLVSRERRGLLRLYVQSAYARDSTHSSSQPK
                     KKKIGFFIIAHNIQSNKEIKVH"
BASE COUNT          600 a          580 c          620 g          549 t
ORIGIN      
        1 gcatgattct gaggaagctg cggcttcttc tcgccctgat tcttgtccgg ttatatcggc
       61 aaccagccgg cgatgacaaa gccaaccatc atcttaccgc tgcctatgtc tgaaaaattg
      121 aattgttcag ggtcgactaa atagtgtccg ctccaacttc cccagctccc gcagtgccag
      181 cgccaggcca ttttgccggg gataagcggc gagcttgcgc aggatcaccg aggccgtaac
      241 cgttcccgtt tttatcgacg ttgcgagacg cagcagttcg gaccaatgat cggtaatgac
      301 acgaagattg atggagaagt ggcctcacaa atttggacaa tgcgataagt gatagtcttg
      361 ctcaacccaa gccgtggtaa tcacggtaaa cagaggagca agcgcatgag aaggacgagg
      421 aggaatcacg cggccggatt taaagccaag gtggcgttgg cagcgctaaa gggtgacaag
      481 acattggcgg agttgtctga acacttcgat gttcacccga atcagatatc cgaatggaag
      541 caacagttga tggaatctgc cccggatgta tttggtggcc cgccaaaagc gaagacggcg
      601 gagcctgact tgaaggtact gcacgccaaa atagggcagc tcacgctgga gaatgatttt
      661 ttagaaagcg cgctcaccaa ggcgggattg ctgagcgaaa agagatgatc gaccgcactc
      721 atgaactgcc aattgttcgg caatgccgga tactggagct atcgcgctcg acggcctact
      781 accagccggg tctggtgtcg ccggacgagt tggcactgat gcgtcggatt gatgagctgc
      841 accttgagca tccgtttgcc ggagcccgga tgttgagccg gatgctcaag cgcgaagatc
      901 agccagtcgg tcggcgccgt gtgtccgcgc tgatgaagcg aatggaaatc aatgcgctgt
      961 atcgtaagcc cagcaccagc aagcgacatc cggcgcataa agtgtatccc tatttgctgc
     1021 gcaatctgac gattacccgc tcgaaccagg tctggtcagc ggacatcacc ttcattccga
     1081 tgaagcgtgg tttcgtatat ttattcgccg tactggactg gtcaagccgc cgggtgctgt
     1141 cgtggcgcgt gtctaatacg ctgaccaccg acttctgcat ggaagccgta caggaagcga
     1201 tcaaccgtta tggcaagccg gacattttca acaccgacca agggtgccag ttcaccagcc
     1261 ttgagtttac tggtctgctg aaagacaaca gcatccagat cagcatggat ggcaaacact
     1321 gttttcattt ttataagtga agccttgcgg tttattgcct acgctgcttt ggcagtacag
     1381 gcaggttgtc gaagtagaac tcatcgggcg ttttatcgtc aagcgccgta tgcggtctgc
     1441 gttggttgta aaacgtaaag tatttctcca accctctttt ggcctcgctg acgctgtcgt
     1501 aagcatgcag atacacttcc tcgtatttga cgctcttcca tagcctttcc acgaatacgt
     1561 tgtcacgcca gcatcctcca aacaaccgaa gccaccgctt ggcacccgcc accagcgcag
     1621 cgagttcggg cggcaccttc atgcccggca atatatagag tcgcttttcc ttcaggtcac
     1681 gaatgcgcgg cgcgaagaga aaccctaaca gccggcacat gacaaagacg tgatccgaga
     1741 aaccgcctgt gtcggtgtag tgttcgctaa tgatgaggct gctttgatgg tagagcagtc
     1801 cgtcgagaac atagggcgcc tcgttggcgg tcgccgcgat cacgttggtg tgataggggc
     1861 caaactgatc ggaaaaatga gtgtagaact ttaaaggatg gcaaaccaga acgtaagtac
     1921 cgcttcctta ttgagtttgg tctacattgt tttacccgag gaccaaacat tgatcatgga
     1981 gaagaactgc accatgttga tagtgcgcta cattattcag attccagaga aaaaaggatt
     2041 tttagttttg accgatacca cctttcattt aagctacccg atatagcaag atcagttagt
     2101 gagtgccctt gctacaatac tggaaaaggt aactttttta taattgattt attggatgat
     2161 caaggacaaa agatagaata tgaggtctac tttctggtat cgagagagcg gcggggtctg
     2221 cttaggctct atgttcaaag tgcctacgcc agagattcga cgcacagctc ttcacagcca
     2281 aagaagaaga aaataggctt ttttattatt gcccacaaca tccagagcaa taaagaaata
     2341 aaggtccac
//
LOCUS       DATNGP010000046         2351 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_1728484, whole genome shotgun sequence.
ACCESSION   DATNGP010000046 DATNGP010000000
VERSION     DATNGP010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2351
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1728484"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..788)
                     /locus_tag="VN778_01155"
     CDS             complement(<1..788)
                     /locus_tag="VN778_01155"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXR49615.1"
                     /translation="MATLLIIALSLLSGTITGAHFSGWAIILLIFSMYRMINLLRLVE
                     GRTQADYLFAVSKRSSLWLIGLQLMVLLLARINHIYHIRSIVWLYALGLFQLVAASTV
                     LASTLRHLRTTKPPAIKEHYADRDLPTLSVCIPARNETEDLEACLQSLIISNYPKLEI
                     LVLDDCSQNKRTPEIIRAFAQDGVRFVDGKVPPERWLAKNYAYKQLVDEASGELLLFC
                     GADTRFQPESLRIMVETLLAKRKTMLSIIPRNKLPSIWDLSSLV"
     gene            1009..1407
                     /gene="secE"
                     /locus_tag="VN778_01160"
     CDS             1009..1407
                     /gene="secE"
                     /locus_tag="VN778_01160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HXR49616.1"
                     /translation="MADKPAKPKRRVVKNPETFRERAIKAVEASEQPKRNNRVRQAVG
                     RFSGPVFRPIGRGLRTIFGFKPVRMLGRVVFPRYFRNSWAELRLVTWPNWRESRQLTF
                     AVLVFAVVFGAAIAGVDWGLDKVFKQLLLK"
     gene            1422..1970
                     /gene="nusG"
                     /locus_tag="VN778_01165"
     CDS             1422..1970
                     /gene="nusG"
                     /locus_tag="VN778_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015642117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HXR49617.1"
                     /translation="MNSSKRYDTSKRWYAIHTYSGYEEKVAESIRQRADSLDMKDKIF
                     EVLVPKEKMIEIKNGKRRVVEKRIFQGYVLVHMKLSEDAWYIVRNTPSVTGFVGSGTD
                     PTPIEQDEIEKIQKRMGLEQPKHKIDFSIGEVVSITDGPFKGFDGSINDIDAAKGKLK
                     VLVNMFGRETPVELDSLQVKRV"
BASE COUNT          670 a          562 c          582 g          537 t
ORIGIN      
        1 ataacaagcg aggatagatc ccaaatactc ggcagcttgt tacgtggaat gatgctgagc
       61 atggttttgc gtttggcaag cagggtttcg accataatgc gtaagctttc cggctgaaaa
      121 cgggtatctg caccgcaaaa aagcaataat tcaccgctcg cttcatcgac tagttgcttg
      181 tacgcataat ttttggccag ccaacgctcc ggtggcactt ttccatccac aaagcggaca
      241 ccatcttgtg caaaggctcg gataatttct ggtgtgcgtt tgttttgcga acagtcatcc
      301 aaaaccaaaa tctccagctt tggataatta ctaatgatca gagactgcag acaggcttcc
      361 aaatcctcag tttcattgcg cgccggtata caaacactta gcgtgggtag gtcgcggtcg
      421 gcatagtgct ccttgatggc aggtggtttg gtggttcgca aatgccggag tgtggatgcc
      481 aaaacagtgc tggcggccac cagctgaaac agccccaaag catacaacca gacaatagac
      541 cgaatatggt atatatgatt tatgcgtgcg agaagtaaaa ccatcagttg caagccgatg
      601 agccataggc tactccgttt agacacggca aatagataat cggcttgagt gcgaccctct
      661 accagccgta gcagattaat catgcggtac atgctaaaaa tcaataggat aatcgcccag
      721 ccggaaaaat gcgcgccagt gattgtgcca ctgagcaggg ataaagcaat aatcaagaga
      781 gttgccatca attgacgcat ccgccataca ggggtaaacc ataaaagcaa ctcacccacg
      841 ctgaccactc ctaatccaat aagcgcaagc atcatatcca tcaactataa actaaaaact
      901 aggaatttta ctagcttgac cagaggtcat gagcttggta tacttgacat cagtggccta
      961 caaatcatgt aggcttttta atttgaggaa tgtgggagcg tcaaagatat ggccgataaa
     1021 ccagcaaaac ccaaacgccg ggtcgtaaaa aatccggaaa ccttccgaga gcgagcgatt
     1081 aaagccgtcg aggccagtga gcagcccaag cgaaacaatc gtgtcagaca ggccgtcgga
     1141 cgtttctccg gccctgtttt caggcctatc ggccgggggt tgcgcaccat ttttggcttt
     1201 aagccagtcc gcatgctcgg gagagtagtg tttccgcgat atttccgtaa cagctgggct
     1261 gagctacgac ttgtaacctg gccgaattgg cgagaaagcc gccaactgac cttcgccgtc
     1321 cttgtctttg ccgtcgtttt cggtgcggca attgccggtg tcgactgggg cctagataaa
     1381 gtctttaagc aacttttact aaaataagga actaggaaga tatgaatagc agcaaacgat
     1441 acgacaccag caaaagatgg tacgccatcc atacctacag cggttatgaa gaaaaagtcg
     1501 ctgagagtat ccgccagcgg gccgatagcc tcgacatgaa agacaaaatc tttgaggttt
     1561 tggtgcccaa agaaaagatg attgagatca aaaacggtaa acgtcgcgtg gtcgaaaaac
     1621 gcattttcca aggatacgtt ctagtgcata tgaagctcag tgaagacgcc tggtatattg
     1681 tgcgcaatac gccgtcagtt accggcttcg ttggtagcgg caccgatccg acgcccatcg
     1741 agcaagacga gatcgagaag atccagaagc gcatgggcct cgagcagcca aagcataaga
     1801 tcgacttttc tatcggcgaa gttgtcagca tcaccgacgg tcctttcaaa ggcttcgacg
     1861 gcagcatcaa cgacatcgac gccgccaaag gcaagctcaa agtcctcgtc aacatgttcg
     1921 gccgcgaaac ccccgtcgaa ctcgacagtc tgcaagttaa gcgggtatag tggtgagccg
     1981 attcgagcac tttctcgaag aattcacagc tcctatggac aaaatcgacg ctgtgtcttt
     2041 tttgggaggt gtggccgtga ccgctgagac ctcgatcgta caaaacttgg atgttctgcc
     2101 tgcgtttgga gtaggtgcag catccgctat cgttttgagc gtgttactca ggaaactggt
     2161 ccatcggaca ctagatgatt cataggcctg gacatacacc gcgaaatcag gtaaaataca
     2221 tctgttacgc acttctgtct taacgcttca attattaagc agaattaaga aagggaagga
     2281 gacggttgag ccaagctccc gtcgaaataa tctatggcaa aagcagttag agctaattta
     2341 aagatgaaat t
//
LOCUS       DATNGP010000047         5606 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_3529352, whole genome shotgun sequence.
ACCESSION   DATNGP010000047 DATNGP010000000
VERSION     DATNGP010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5606
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3529352"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(172..834)
                     /locus_tag="VN778_01170"
     CDS             complement(172..834)
                     /locus_tag="VN778_01170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49618.1"
                     /translation="MTKFRFKKVPANLGLIRHTEKERLIPDDIYEYAKSFRSVYTRSM
                     PVLKKIADPKWASHQEIKQLYGKPQSLAVQIALSNELLLKAILLGSTGEIIKEHNLKK
                     LINNLDTRYINIIKQHLEENGLKEGRWNNVLNMSAQTFVDTRYGFEGKAYDIDFVTHQ
                     LLNEVLDDIFNNYLPDWTRLTQVQQENKERLKKEVDLIFDEDYQKEQAEQMKLWHKAF
                     KK"
     gene            complement(885..2249)
                     /locus_tag="VN778_01175"
     CDS             complement(885..2249)
                     /locus_tag="VN778_01175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49619.1"
                     /translation="MTDDLFQMLESKGITDGPTTFEIIGEYTYEEMMDLAADCFLAIK
                     NIEKQPKSKLFSFAPIKSIAGGPYPCTSVECRLDAAYSFSLVAALYAEKVILPNFFDY
                     MFYILEGEREPPQTQQAIEDFEYNLCADIAVCYFYKPLFEAGIAEMNFTVPTVCKDCH
                     ARHLKETEEFSNNLEKAIKMTEPIFKSKVKFIYQNNDYLEVANEGGYLGGELGYSIRH
                     TLKAAMRKHKPQIPYQLSENEINRFGLREHLIDLCVDDILEYDFYPSLNSSTYLTNRQ
                     FDVDLIEASNKHDESILMTSHMPAFHQLPFLQDISIEDVVKLRQAEDKSFKVYRNAVA
                     KVVNAKNSEAAAKYMETELKPALDNIDNIITQTQKHYRALAGKKITKRTIVAVAGIGA
                     ANLLNIPVQASIPALGTAGLFSTQDILDDLKAGKDIPLDAKVDPYYFLWGIKQQHEAS
                     QPHD"
     gene            complement(2509..3630)
                     /locus_tag="VN778_01180"
     CDS             complement(2509..3630)
                     /locus_tag="VN778_01180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49620.1"
                     /translation="MSRKIFVTVASGGHNTEQHYQDTIKSQRTVDEVAQFLSSETVEE
                     LKKYTHNRPYAVWGAVTGLGNTRTWQTMERGDYVIVYRSKHIIGVAEVAYKIHSPQLA
                     KYFWGEDAEGRTWEYVYFLTNFMETNVEQSKLNQYLGYVSHYTFQGFSAVDQKKTDAL
                     LAGYGDLISVLKKLEANEKIEAISIDKLFQKQIVDQEIKKQIERAETPHDEMQWRLIS
                     LGNKARLDVWVPVNDQGKSYQGNMFRPHVIDSFGEAVDIPSYIKNIDTVWKLGLSIKA
                     AFEIENSTAIYSGILRLSDLRALAPNSSYPLYIVADRAKRARVFEQINRPTFTNDYLQ
                     LEKVVKYLSYDAVRDLDQSASLDKLYDISAVETAAEAAS"
     gene            3812..4828
                     /locus_tag="VN778_01185"
     CDS             3812..4828
                     /locus_tag="VN778_01185"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HXR49621.1"
                     /translation="MSVLAESADKTVQQTTNVFQRIVHGVFNAHSLTVFVVSIVVALL
                     AGRFIAYLLRRIVVLISRQADKSPDLGTVNRLRRYETYIVISIAMIRTLLVIFAIYFW
                     WQYVHPQAQPTAIVGASALIAILLSGALAPVLRDLAFGSFMMAEQWYGVGDYIKIEPF
                     LDIEGVVERVTLRSTRLRTLNGEIVWLSNQNIAGIHVTPKGIRTIGLELFVDNPEAGE
                     RLIAKANRRLPIGPLLVVTPLTVVSNEKVGDNLWHISAIGETAPVREWLIEKSAVEII
                     KWMDSQSKKPTIAHGPLARYADPAAERSFRRTVENARKRPTPKRRGIKRKPSQDKPIR
                     SSSS"
     gene            complement(4836..5375)
                     /locus_tag="VN778_01190"
     CDS             complement(4836..5375)
                     /locus_tag="VN778_01190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49622.1"
                     /translation="MSARNPYEHNDVVPEVDPQVTASFVNVVHMLTGVNLETRLKSYR
                     IIDRADFTPLRICANLLGYANDDLIDDKVERSKAAVIERHSRTELWQHPEDKFRHYWN
                     VVESVYINDLGEPTHQKSYWRRGLFGRLRIVDFDYQATESQVVEARDETIDAAMRAGL
                     LAVNPERFFAGAPRPANQE"
     gene            complement(5410..>5606)
                     /locus_tag="VN778_01195"
     CDS             complement(5410..>5606)
                     /locus_tag="VN778_01195"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014818069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA-guanine transglycosylase"
                     /protein_id="HXR49623.1"
                     /translation="QNYTRAYLHHLLHAKELLAHTLMSIHNEHFIIKLVEDIRAAIDD
                     NSFFELKKAWLNRYYSQSAH"
BASE COUNT         1451 a         1232 c         1223 g         1700 t
ORIGIN      
        1 tttaaattat gcacacaatg tggcacttgt ctatagtgct tatgctaaac caagtgtggc
       61 aataattttg gtgcagcact attcgtgaaa gtgtaacttt tctcaggggt gtggcataag
      121 gttatatcca acagatgctg ggcattatca atgaagttat aactaacaaa attacttttt
      181 aaaggcttta tgccacagct tcatttgttc tgcctgttcc ttttggtaat cttcgtcaaa
      241 tattaaatca acttctttct ttagtctttc cttattttct tgttgtactt gtgttaagcg
      301 agtccagtca ggcagatagt tgttgaatat gtcatccaac acctcattaa gtaattgatg
      361 cgttacaaaa tctatgtcat aagccttacc ctcaaaacca taccttgtat ctacaaaagt
      421 ctgcgctgac atatttaaca cattattcca acgaccttct ttaaggccat tttcttctag
      481 gtgctgcttg atgatattga tatatcttgt gtctaaatta tttattaact tttttagatt
      541 atgctcctta ataatctcgc cagtgcttcc tagtaaaatc gcttttagta aaagctcgtt
      601 tgataaagca atctgcactg ctaagctttg tggcttgccg tacagttgtt taatttcttg
      661 atgactagcc catttgggat cagcaatttt tttaagtact ggcatggagc gtgtataaac
      721 actacgaaat gatttcgcat attcataaat atcatcagga attagtcgct ctttttcagt
      781 gtgcctgatc aagccaaggt ttgcaggcac tttcttgaat ctaaatttag tcatacccga
      841 attgtatacc gcttacttaa gcctcacaaa aatgatcaag cagcctaatc atgtggctga
      901 cttgcttcgt gctgctgttt aatcccccag agaaagtagt aaggatcgac tttagcatca
      961 agcggtatgt cctttccggc ttttaggtca tccaaaatat cttgagtaga gaataaaccc
     1021 gcggttccca atgcaggaat cgatgcttga accggtatgt tcagaaggtt tgcagcgcca
     1081 atcccggcaa ccgcaacgat tgttcttttt gttatttttt tgcccgcaag tgctcgatag
     1141 tgcttttgtg tttgcgtgat tatattgtca atattgtcta aagctggttt cagctctgtc
     1201 tccatatact ttgcggctgc ttcagaattt ttggcgttaa ctactttagc aactgcgttc
     1261 cgatatacct taaagctctt atcctctgcc tgacgaagtt taactacatc ttctatgctt
     1321 atgtcttgca gaaagggcag ttgatggaaa gccggcatgt ggctagtcat aagtatggat
     1381 tcgtcatgtt tattagaggc ttcaattaga tccacatcaa actgcctatt tgtcagatat
     1441 gttgagctat ttaggctcgg ataaaagtca tactctaaaa tatcatccac acataaatcg
     1501 atcagatgct cccttaagcc aaacctgttt atttcatttt ccgaaagctg gtatgggatt
     1561 tgaggtttat gcttacgcat tgcggcttta agcgtatgtc gtatagagta tccaagctca
     1621 cctcctaaat agccaccctc attagcaact tcaaggtagt cattattttg gtagatgaat
     1681 tttacctttg acttgaaaat tggttcagtc atctttatag ctttttcgag attgttactg
     1741 aactcttcag tctcctttag gtgccttgcg tggcaatcct tacacactgt aggaacggta
     1801 aaattcatct ctgctattcc cgcttcaaaa agaggcttat aaaaataaca aaccgcgatg
     1861 tcggcgcata agttatactc gaaatcttct attgcttgtt gggtttgggg tggttccctt
     1921 tctccttcaa gaatgtaaaa catatagtca aaaaagttag gtaaaataac tttttctgcg
     1981 taaagtgcag ctactaatga aaatgaatag gcagcatcga gcctacactc aacacttgta
     2041 cacggataag gaccgccagc aatcgatttg atgggggcaa agctaaacaa cttggattta
     2101 ggctgtttct caatattctt tatagcaagg aagcaatcag cagcaagatc catcatctct
     2161 tcatatgtat actcacctat aatttcaaat gttgttggcc cgtcagttat tcctttgctt
     2221 tctagcattt gaaagagatc atctgtcatg ctgaaattat aaccctatct gtgcagataa
     2281 gcgctaattt aggatcatga ggttataacc gttgtttcaa tctgtaaatt gctacttttt
     2341 gtctcgtgag gtctatctca gtggacgtaa gttgaaaccg cgccaccgat caataaacaa
     2401 aaagctcccg aacaagagag cttcctaata ttgtcacttt ggtgcagcag cattgaccaa
     2461 agtgtaacca ttttgagaca atcgttgcgc tatagctatt cgtctttgct aacttgctgc
     2521 ttctgctgca gtttctacgg cactaatatc ataaagttta tctaaggagg cagattggtc
     2581 taagtcacgg acggcatcat acgataagta cttgactact ttttcgagct gaaggtaatc
     2641 attagtaaag gtcggacgat tgatttgctc gaatactcta gcacgttttg cccggtcagc
     2701 tactatgtac agtgggtagc ttgagtttgg cgctaatgca cgcaagtctg ataggcgtaa
     2761 tatgcctgag tagatggctg tagaattctc aatttcaaaa gcagctttga ttgaaagccc
     2821 cagcttccag accgtatcaa tatttttaat atagctcggg atatctaccg cttcaccaaa
     2881 tgaatcaatt acgtgggggc gaaacatatt accttggtag cttttaccct ggtcattgac
     2941 cggcacccag acatctaagc gcgctttgtt tccaagactt atgagccgcc attgcatttc
     3001 atcatgaggc gtctcggcgc gctcgatttg tttctttatc tcttggtcca caatctgttt
     3061 ttggaataat ttatctatgc taattgcttc aattttctcg ttcgcttcca acttcttaag
     3121 cactgagatt aaatcgccgt agccagcaag taaggcgtcc gtctttttct ggtcaactgc
     3181 actaaaccct tggaaagtgt aatgcgaaac atagccaaga tattgattga gcttactttg
     3241 ctcaacgttt gtctccataa aattagtcag gaaataaaca tattcccacg tgcgcccttc
     3301 tgcatcttcg ccccaaaaat acttagctaa ctgcgggcta tggattttat aagcaacttc
     3361 agcaacaccg ataatatgct tgcttcggta aacgattaca taatcacccc gttccatggt
     3421 ctgccatgtt cgggtattgc cgagtcctgt tacagcaccc caaacagcgt aaggccgatt
     3481 atgcgtgtac ttctttaatt cttcgacagt ttctgagcta agaaactgcg ccacttcatc
     3541 aacagtacgc tgggatttaa tagtgtcctg gtagtgttgc tcggtattat ggccaccaga
     3601 agctactgtt acaaaaatct ttctactcat tatgaaaatg ttaacattta atcgttatta
     3661 aacataagtt tactctttgc cataaaactt gaagctcaag gagtttttca gtatagtaga
     3721 gtataagttt tgcaagccat gagtggctgc ttggcaacaa acaaatgggc gtttggttag
     3781 caaacgcttt ttaaatgcga gagaggttcc cgtgagcgtg ttagcggaga gtgcggataa
     3841 gacggtgcag cagacgacga atgtgtttca gcggattgtg catggggttt ttaatgcgca
     3901 ttcgctgacg gtgtttgtgg tgtcgattgt ggtggcgctg ctggcggggc ggttcattgc
     3961 gtacttgctg aggcggattg tggtgctgat cagccggcag gcggataaga gcccggacct
     4021 ggggacagtg aatcggctgc gacggtatga gacttacatc gtgatcagta ttgccatgat
     4081 ccggacgttg ctagtgatct tcgctattta tttctggtgg caatatgtgc atccgcaggc
     4141 tcagccgacg gcgattgtgg gggctagcgc gctgattgcg attttgttga gcggggcgct
     4201 ggcgccggtg ctgagggatt tggcgttcgg gtcgtttatg atggctgaac agtggtacgg
     4261 cgttggcgac tacatcaaga ttgagccttt cctggatatc gaaggcgttg tcgagcgggt
     4321 gacgctgcgc tctacccggc tgcgtacctt gaacggcgag atcgtctggc ttagcaacca
     4381 gaacattgcc ggcatacacg tcacgccaaa aggtattcgc actatcgggc tcgagctgtt
     4441 tgttgataat cctgaggctg gcgaacgatt gatcgccaaa gccaaccggc gcttaccgat
     4501 tgggccgttg ctggtcgtga cgccgctgac ggtcgtatca aacgaaaaag ttggtgataa
     4561 tctgtggcac atctctgcta tcggcgaaac cgccccggtt cgagagtggt tgatcgaaaa
     4621 gtctgcggtc gaaattatca aatggatgga tagccagagc aaaaagccga cgatcgccca
     4681 cggcccgctg gcccgctatg ccgacccggc cgccgaacgc agcttccgtc gcaccgtcga
     4741 aaacgcccgc aaacgcccga cgccgaagcg ccgcggtatc aagcgcaaac cctctcaaga
     4801 caagcctatt cggtcgagtt cgtcgtaaaa acgtcttact cttgattcgc cggtcttgga
     4861 gcgcccgcaa aaaaccgctc cggattaaca gcgagtaggc cagccctcat cgccgcatca
     4921 atagtttcat cacgagcttc aacaacttga gattcagtcg cttgataatc aaaatctaca
     4981 atgcgtaggc gtccaaacag tcctcttcgc caatatgatt tctgatgagt cggctcaccc
     5041 aaatcattga tataaacact ttcgaccaca ttccaatagt gtctaaactt atcttcggga
     5101 tgctgccaca gctcagttcg gctatgacgc tcaataacag ctgctttact ccgctctact
     5161 ttgtcatcaa ttaaatcgtc gttggcgtag ccaagcagat tagcgcatat ccggagtggt
     5221 gtaaaatctg cccgatcaat tatgcgataa ctttttagtc tcgtctccag gttcacacct
     5281 gtaagcatat gaactacgtt gacaaaagac gcagtaactt gaggatctac ctctggtacg
     5341 acatcattgt gttcgtaggg gttccttgca ctcataagcc atgtattata ctactaatgt
     5401 ttttatttgt caatgagcag attgtgaata ataacgattt agccaggctt ttttgagctc
     5461 aaagaaggag ttatcgtcga tggcagcccg aatgtcctcg acaagcttaa tgatgaaatg
     5521 ctcgttatga atcgacatca aggtatgggc cagcaactct ttggcgtgca acagatgatg
     5581 cagataagcg cgggtgtagt tttgac
//
LOCUS       DATNGP010000048         2830 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_4404041, whole genome shotgun sequence.
ACCESSION   DATNGP010000048 DATNGP010000000
VERSION     DATNGP010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2830
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4404041"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            98..256
                     /locus_tag="VN778_01200"
     CDS             98..256
                     /locus_tag="VN778_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006341780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6496 domain-containing protein"
                     /protein_id="HXR49624.1"
                     /translation="MPKYDPKSRKEVEKTMHEHKHGGKFKNRQQAVAVGLSKARQKGE
                     KVPEKGDS"
     gene            complement(253..1467)
                     /locus_tag="VN778_01205"
     CDS             complement(253..1467)
                     /locus_tag="VN778_01205"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HXR49625.1"
                     /translation="MLDTAHALIRQVGQRLGLDEAAIEELISIDAEHSFDIELSSGKS
                     VKAYRVQHSSKRGPYKGGIRFHPEVTLDEVRALATLMSFKTAAVGLPLGGGKGGVVVN
                     PRELDDKELEEISRKYVDHLQPHIGPDKDIPAPDVNTDSRIIDWMVDEYEILTGDTSK
                     ASFTGKSLARGGSEGREAATGRGGVIVLHELLKHEGLSQKDLRLAIQGFGNVGSFFAT
                     IAKDEEPHWHLAAASDSGATLQTAGQLNADKLSELKRQRGRFEDYKAEGVQVADAEAI
                     IGSDVDVLVLAALGDAITEVNMHQVKAKYILELANGPVNTKAYEYLSGQGKIIIPDII
                     ANAGGVVVSYLEWLQNKAHEHWPEAEVNRRLQEYLVKAARALHQTASKQNATLKEAAF
                     INALARLRQAED"
     gene            1515..1847
                     /locus_tag="VN778_01210"
     CDS             1515..1847
                     /locus_tag="VN778_01210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49626.1"
                     /translation="MALSTTTGSGTQTTTQSPQASTGTTGTTESSSSVQPGTATSLLS
                     SQNGMPLHASALTTVNLTGATQAQTSQAAPPSKHHINGGLLGISLALFVIAIALFWFT
                     SRPVKSTT"
     gene            complement(2148..>2830)
                     /locus_tag="VN778_01215"
     CDS             complement(2148..>2830)
                     /locus_tag="VN778_01215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49627.1"
                     /translation="KLLSKVDLMNTKSTPALPQAARFCQIFAWGLALIVATAAVVTWG
                     QAYQWHVLPINNYVLFPLFGLLAFSIMWSHYVAGAVRELLKLDKSTLKSYFEGTSFVV
                     LALICLHPGLLMYQRFRDGFGLPPHSYETYVAPGLGWVTLLGTLCLGIFLAYEFRRKY
                     GRRPWWRHMQELTDAAMLAIFYHALRLGHQIIGWFRVVWWFYGIVLAIILARTYTMKY
                     FAHKKDPA"
BASE COUNT          734 a          728 c          710 g          658 t
ORIGIN      
        1 ggctcggcgg tcagcacagc catcgcaatc gttaggtaat aatcaggctg ccacgcacat
       61 tacgggagaa tagcggtagc ttctcgggag gataatcatg ccaaagtatg atccaaagtc
      121 gcgcaaagaa gtcgaaaaga ccatgcatga gcataagcac ggggggaagt ttaaaaaccg
      181 gcagcaagcc gttgctgttg gcttgagtaa agctcggcaa aagggagaaa aggtgcccga
      241 aaagggcgat agttagtcct cggcttggcg taagcgggct agcgcgttta taaacgctgc
      301 ttctttgagg gtagcgttct gcttgctcgc ggtctggtgg agcgcccggg cggctttgac
      361 gaggtattcc tgaagccgcc ggttgacttc ggcttcgggc cagtgctcat gggctttgtt
      421 ttggagccat tccagatagc tcaccacgac accgccggca ttggcgatga tgtcagggat
      481 aatgattttg ccctgtccgc tcaagtattc ataggctttg gtattaaccg gcccgttggc
      541 tagctctaga atatacttgg ctttaacctg atgcatgttg acttccgtga tggcatcgcc
      601 gagggccgct aggaccaaaa cgtcgacatc gctgccgatg atagcctcgg cgtccgctac
      661 ttgcacgcct tctgctttat aatcctcgaa acgtccccgc tgccgtttga gttcgctaag
      721 tttatctgca ttcagttggc cggccgtttg cagggtggca cctgaatcgc tggcggctgc
      781 caggtgccaa tgcggttctt cgtctttggc gatggttgca aagaatgagc cgacattgcc
      841 aaagccttgg atggcgaggc gtaagtcctt ttggctcaag ccttcgtgtt tcagtagttc
      901 gtggagtacg atgacgccgc cgcggccagt cgcggcttcg cgcccttcac tgcccccgcg
      961 ggccagactt ttaccggtga agctggcttt gctcgtatca ccggtgagta tttcgtactc
     1021 gtcaaccatc caatcgatga tacggctgtc ggtgttaacg tctggcgccg gtatatcttt
     1081 gtcgggaccg atatgcggtt gcaaatgatc aacgtacttg cggcttattt cttctagctc
     1141 tttgtcatca agctcgcgcg gattgactac cactccccct ttaccgccac cgagcggcaa
     1201 gccgacagcg gcagttttga agctcattag cgtggctaag gctcgcactt catcgagagt
     1261 aacttcggga tggaagcgga tgccaccctt gtaagggccg cgtttgctgc tgtgctggac
     1321 gcggtaagct ttgacgcttt taccactcga tagttcaata tcaaaactat gctcagcatc
     1381 gatagaaata agttcttcaa tagcagcctc atcgagacca aggcgctggc caacttgtcg
     1441 aataagcgcg tgagctgtgt caagcataca tctctattgt acaaccaaca ccatattgct
     1501 ttacaataat atgaatggct ttatcaacca caacagggtc tgggacgcaa accacgacgc
     1561 agagtcccca ggcctcgacc ggcactactg gtactacgga gtcgagtagc agcgtacagc
     1621 ccggtacggc gactagcctt ttgtctagcc aaaacgggat gccactacat gcctctgctc
     1681 tgacgacagt taatctcacc ggtgctacgc aagcgcagac cagtcaagcc gcacctccct
     1741 ctaagcatca cattaatgga ggtcttctcg gtatatcact agcattattt gtgatagcca
     1801 tcgcactatt ctggttcacg agccgaccgg taaaaagcac aacgtaatac ttagaatgtg
     1861 cttgcacagc agcaagctta agcagtatca taacgcctga gatcaagcga aacacagcat
     1921 atctccgcac ataaattgac aaaatttcaa aaatgtgtat aatacatagt gtttcgtgat
     1981 cacaaacaaa aatcattaca aaaacaaaag gaaaggaccg tgtcctctat ggcagccaaa
     2041 aatgctctag ctttcgagct agttaatacc tccgataaag gtattttgcg aattgatcgg
     2101 ctattgaaac tacaataagt tctatagacg tcaaaaagac ccggatttta agccgggtct
     2161 tttttgtggg caaagtactt catggtatag gtgcgtgcga ggattatagc gagtacgatc
     2221 ccatagaacc accacactac ccggaaccag ccgatgatct ggtggccaag gcggagggcg
     2281 tgataaaaaa tagccagcat ggcggcatct gtcaattctt gcatatgacg ccaccaggga
     2341 cgccggccgt atttacgacg aaactcgtag gcaagaaaaa tacccaagca cagggtaccg
     2401 agaagtgtta cccagcctaa ccccggcgcc acatatgtct cataactatg cggcggcagg
     2461 ccaaacccgt cccggaaacg ttgatacatc agcaggcctg gatgcaagca gatcagtgcc
     2521 agaaccacga aactggttcc ctcaaaatat gacttcaaag tacttttatc gagctttagg
     2581 agctcacgga cggccccagc aacatagtga gaccacataa tgctaaaagc caacaaccca
     2641 aaaagcggaa aaagcacata gttattgatg ggcagtacat gccactgata agcctgcccc
     2701 caagtcacca ccgctgctgt agcgacaata agagccaaac cccaggcaaa aatctgacag
     2761 aaacgagcag cttgcggtaa ggcgggtgtc gatttcgtgt tcattaaatc tactttggat
     2821 aaaagcttat
//
LOCUS       DATNGP010000049         4061 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_1985733, whole genome shotgun sequence.
ACCESSION   DATNGP010000049 DATNGP010000000
VERSION     DATNGP010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4061
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1985733"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="VN778_01220"
     CDS             complement(<1..225)
                     /locus_tag="VN778_01220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49628.1"
                     /translation="MASIPILSNNNGERVAANVDAQQAGTVAPSARTPSLRAGLGEMA
                     RTFFHSGPSQYQFHEGAGRWYNTHEPADFDA"
     gene            475..1533
                     /gene="tsaD"
                     /locus_tag="VN778_01225"
     CDS             475..1533
                     /gene="tsaD"
                     /locus_tag="VN778_01225"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HXR49629.1"
                     /translation="MKVLGIETSCDETSAAVVEDGYRLLSNVVASSADLHVQYGGVVP
                     EIAARSHIEVINQVIAQALDDAKTDWNDIGAIAVTYGPGLGGSLLIGVMTARTLAIAH
                     NKPLYAVNHVEAHAYANFLTATPLPGYLLHETQPEFPMLALLVSGKHSEHVLFHDHFQ
                     HTLLGSTQDDAIGEAFDKVAKMLGLPYPGGPSVGKKALEGDSSRYELPKAHVGKYDFS
                     FSGIKTAVLRLAQSEIGEDLTFPSKDLPERLSERQKADIAASFQRVAIETIVDKTIAA
                     YEEFAPRSVVIGGGVAASPELRRQLAERLPYKINYPDMKLCTDNGAMVATLGCLMHEL
                     GQPQADPYTLDINPSLHL"
     gene            1978..>4061
                     /locus_tag="VN778_01230"
     CDS             1978..>4061
                     /locus_tag="VN778_01230"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HXR49630.1"
                     /translation="MDLPKTYEPGQYEADIYALWEKTEAFKPKGKGKPYSIVMPPPNA
                     NANLHIGYALVGAIEDIAARYHRLRGDNTLLLPGADHAGFETQSVYEKKLAKEGKSRF
                     DFSREDLYKQIWDFVAENRTNFLTQYRRLGLSCDWGRFTFTLDEKIVKRAYATFKKMW
                     DEGLIYRGERLVNYCTFHRTGFADIEVVHEDVETPLYYIKYGPFTLATTRPETKFGDT
                     AVAVHPGDKRYEKYVGQVIEVQGVNGPFSVQVVADEMVDPNFGTGVVKITPAHSFDDW
                     EVAQRHNLPAKRVINHDGTMNHEAGRFEGLSVMDARKAVVAAMKEQGLLEKVDKHYQT
                     RIGKCYKCGTVIEPMLMEQWFLNVRPLADEAIKHLEAKEITFYPDTKRTQLVGYLKGL
                     HDWNLSRQIAWGIPIPAFQNVDDSDDWIYDERVDQEIIEVDGKTYHRDPDVFDTWFSS
                     SSWPYATLGYPGSDDYKTFYPLSLMETGGEILYPWVSRMIMLGLYVTGKIPFEEVYIH
                     GYFMAEDGSKMSKSLGNVVDPMPVIDQYGSDALRMGIIAGRAPAVNRGYDTRRVEEAR
                     NFCNKLWNVARYIQGLAGDTAGEGRAEPKAPADHWILTKLHDLQTGLEGDLAQYRLSE
                     AYERLYHFVWDDLADWYLEASKAAPNLPLLMEILRQTLIMIHPFAPFVTETIWQTLES
                     TGDSVLAVQPWP"
BASE COUNT         1049 a         1038 c         1078 g          896 t
ORIGIN      
        1 ggcatcaaag tcagccggct catgggtgtt ataccatctg cctgctcctt catggaattg
       61 gtactggcta gggccactat gaaagaatgt tcgggccatt tcaccgagac cagcgcgcaa
      121 acttggtgtt cgcgcagaag gcgccactgt gccagcttgc tgcgcgtcaa cattcgccgc
      181 tacgcgctcg ccgttattgt tggataagat tggtatgctt gccataaaat atttcctatt
      241 cttttgcttt tttgtttatg tcaattatta tacacagaat cgtgcttatg tcaataacta
      301 tataataact ttcgcgatag cgtaactcga tgtagctgta ggcttaataa gaacaactct
      361 ttgtgaaata tctcacaatt ctgctggaat aatactaccc cctgcaccag ctatgcaaca
      421 gacgtgaatt gtgcccaaga actcctctgg agctgataca atacagtgtg aactatgaaa
      481 gttttgggaa ttgagacgag ttgtgacgag acgagcgcgg cggttgtcga ggatggctac
      541 cgactgctca gtaatgtagt tgcctctagc gccgacctgc atgtgcaata cggtggcgtg
      601 gtaccggaaa tcgcggcacg cagccacatc gaagttatta accaggttat cgcgcaggcg
      661 ctggacgatg ctaaaactga ttggaacgat attggcgcta ttgcagtaac atacgggccg
      721 ggacttggtg gtagcttgct gatcggtgtc atgacggcac gtactttagc cattgcacat
      781 aacaagccac tgtacgccgt aaaccatgtc gaggcgcatg cctacgctaa cttcctgact
      841 gccacgcccc tgcccggcta tctattgcac gagacgcaac ccgagttccc gatgctcgcc
      901 ctactcgttt ccggtaagca cagcgagcac gtactattcc atgatcattt ccagcatacg
      961 ctacttggat caacccagga cgatgccatt ggcgaagcct ttgataaggt agccaaaatg
     1021 ctgggtctac cctaccctgg cggcccaagt gtcggtaaga aagcactcga aggtgactct
     1081 agcagatatg agctgccgaa ggcacacgtt ggaaagtacg acttttcatt tagcgggatc
     1141 aaaacagctg ttttacggct tgcccaaagc gaaattgggg aagatctcac attcccgtct
     1201 aaggacctgc cagaaaggct ctctgaacgt cagaaagccg acattgcagc cagcttccaa
     1261 cgcgtggcga tcgaaacaat cgtcgacaaa acgattgctg cctacgaaga gtttgcgcca
     1321 aggtcagtcg tgatcggtgg tggtgtcgcg gctagcccgg agctccgacg ccaactggcc
     1381 gaacggctcc cctacaaaat taactatcca gacatgaagt tgtgtaccga taacggtgcg
     1441 atggtggcga cgctgggctg ccttatgcat gaattaggac aaccgcaagc tgacccctat
     1501 acgctcgaca tcaacccttc cctgcactta tagtctcgtc attcccgcgc aggcgggaat
     1561 ccaggctcat taattactgg attcccgatc ctgtcgggaa tgacgaacga atatgaaaaa
     1621 tttatgaatt atggcttcta gcgcttgaaa agcataagcg gcggagagat aattgaactg
     1681 aaaacaaaaa cgttcgggtt agagaaaacg gtagaaggga actagacatg acggcttcgg
     1741 gaaaaagctg tggtgtttgg ttctgttttc atgtgaaaac ttttttgtaa cgtaaattca
     1801 cccctgttcc caatctggcc agaaggtgat attattgggt ctatgaaatg gctagaaatc
     1861 agcctaaaaa cagctctggc ggtcgcccaa ccgcatgtct cagcacgtga tcgcgtcgcc
     1921 ggcggcgact actttttctc actcgaaact ctcacttact aaacaaggat taactacatg
     1981 gatttgccaa aaacgtacga acctggccag tacgaagcgg atatttacgc gctgtgggaa
     2041 aaaaccgagg cctttaagcc gaaagggaag ggcaagccct actcgatcgt tatgccacca
     2101 cccaacgcca acgccaacct gcatattggc tacgcgctgg tcggagctat cgaggatata
     2161 gccgcacgct accaccgctt gcggggcgac aatacgctat tgctacctgg cgctgaccac
     2221 gccggttttg aaacgcagtc agtgtacgag aagaagctcg ccaaagaagg taagagtcgg
     2281 ttcgacttct cccgagaaga cctgtacaaa cagatctggg attttgtggc cgagaaccgc
     2341 actaatttct tgacgcaata tcggcgactg ggcctgagct gcgactgggg tagatttacc
     2401 tttacgctcg atgaaaaaat cgtgaaacgg gcttacgcca cattcaagaa aatgtgggac
     2461 gagggactga tttaccgcgg cgaacgcctg gttaactact gtacgttcca ccgcaccggc
     2521 tttgctgata tcgaagtggt acacgaagat gttgaaacac ccctgtatta catcaagtat
     2581 gggccgttta cgctcgctac cacacgaccc gaaacgaaat tcggcgatac ggccgttgcg
     2641 gtgcatcctg gcgacaaacg ttatgagaaa tatgtcgggc aggtgattga agtccaaggc
     2701 gtcaacgggc cgttcagcgt gcaagtcgtg gcagatgaaa tggttgaccc taacttcggt
     2761 acgggtgtag ttaaaattac gccagctcat tcatttgacg actgggaagt ggcgcaacga
     2821 cacaacctgc cagccaagcg cgtgattaat catgacggta cgatgaacca tgaagccggg
     2881 cgatttgaag gcctgagcgt gatggacgcc cgcaaagcag tcgtggcggc catgaaagag
     2941 cagggactgc tagagaaagt cgacaagcat taccagacac gcatcggcaa atgttacaag
     3001 tgcggcacgg ttatcgaacc aatgctcatg gaacagtggt ttttgaatgt ccggccgctc
     3061 gcggacgaag caatcaaaca cctcgaagcg aaagaaatta cgttctaccc agacacgaaa
     3121 cgaacgcagc tcgttggcta tctgaagggc ctgcacgact ggaacttgag ccgccagatt
     3181 gcctggggga ttccgatccc ggccttccaa aacgtcgacg atagcgatga ctggatttac
     3241 gacgagcgcg tggaccagga aattatcgaa gttgatggca agacctacca ccgcgatcca
     3301 gacgtattcg acacctggtt ttcgagcagc tcctggccgt acgctacgct tggctacccg
     3361 ggcagtgacg actacaagac gttctacccc ttgagcctga tggaaacggg gggcgagatc
     3421 ctgtacccct gggtcagccg catgatcatg ctggggctct acgtaacagg gaaaatccct
     3481 tttgaagaag tctacatcca tggctacttt atggccgaag acggctcgaa gatgagcaag
     3541 tcactcggta acgtcgtcga ccctatgcct gtcatcgacc agtatggatc ggatgcgctg
     3601 cggatgggca ttattgctgg acgggcacca gccgtgaacc gcggctatga cactcgccgc
     3661 gtcgaggaag cgcggaattt ctgcaacaaa ctctggaatg tggcgcgcta tatccaagga
     3721 ctggctgggg atacggcagg ggagggcagg gcggagccaa aggcgccagc tgaccactgg
     3781 attctgacta aacttcatga tttacaaaca gggctcgaag gtgacctggc ccaataccgc
     3841 ctgtcggaag cctacgaacg cctgtaccac tttgtatggg acgacctggc agattggtac
     3901 ctagaagcca gcaaggccgc cccaaacctg ccgctgctga tggagatttt gcggcaaacg
     3961 ctaatcatga ttcatccgtt tgcgccattt gtgacggaga caatctggca gacactggag
     4021 agcaccggtg atagcgtact ggcggtacag ccatggccag a
//
LOCUS       DATNGP010000050         4153 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_3184156, whole genome shotgun sequence.
ACCESSION   DATNGP010000050 DATNGP010000000
VERSION     DATNGP010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4153
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3184156"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="VN778_01235"
     CDS             complement(<1..218)
                     /locus_tag="VN778_01235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49631.1"
                     /translation="MNKKRLHHIWTKVRQVSPWYFLALTIVSTAICLFALRANDEHMI
                     RLRTAVYAADKNNGDIKGTLQKLQAYVTT"
     gene            272..1567
                     /locus_tag="VN778_01240"
     CDS             272..1567
                     /locus_tag="VN778_01240"
                     /inference="COORDINATES: protein motif:HMM:NF013396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HXR49632.1"
                     /translation="MHIYFSGIGGAGIGPLALIAHQAGYTVSGSDKQDSQHIQYLKRH
                     GIANIHIGQTEAAIAEVYERQPIDWFVYTSALPLENPDAPELAYCDEQDIKTSKRDEL
                     LNQILRDRQLKLIAIAGTHGKTTTTAITTWLFKQLGLPVSYLLPAKTSFAEMGEFNPK
                     GEYFIYEADEFDRNFLSYEPAISLITGVSWDHHELFPTREDYQAAFREFIDQSQRTYI
                     WQEDVDYLGLEVATDVHVLNSASPKIESLKLLGKYNRLDAWLAIQAVHSVTGKPVEQL
                     VDLANRFPGLSRRMEQIIPNLYSDYAHTPEKIRGAMSVALEMAAESGQNVVVVYEPLT
                     NRRQHYMLDEYRDCFAGASKLYWIPSYLAREDPAQRIIPPSELISHLSDPSIAEPMER
                     DAALKKTIQKHLDHGDMVVGMAGGGGNSLDDWLREQFKS"
     gene            complement(1574..2026)
                     /locus_tag="VN778_01245"
     CDS             complement(1574..2026)
                     /locus_tag="VN778_01245"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HXR49633.1"
                     /translation="MSGRHDGVETPYTAVYLIFKKDGKIAFLLRSNTGWMNGHYSLVA
                     GRVDKGEPFTRSAIREAYEEAGATIQPQDLKPVLIIHRNEPGSVWVDLVFEVLKWGGE
                     LYNAEPEKHGELVWFDPNQLPENMVPSARFYIEQYLAGSSYAEYGWNK"
     gene            complement(2023..2355)
                     /locus_tag="VN778_01250"
     CDS             complement(2023..2355)
                     /locus_tag="VN778_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010262862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase
                     family protein"
                     /protein_id="HXR49634.1"
                     /translation="MTFDEYQKQAMTTVLTTGDEFKDLLHWVLGITGEAGEVAEKLKK
                     IVRDKHGKLDDEDKQEIAKEIGDVLWYLAVCAHDLGFSFDEIAAKNIAKLKSRQARGV
                     LGGEGDNR"
     gene            complement(2352..2921)
                     /locus_tag="VN778_01255"
     CDS             complement(2352..2921)
                     /locus_tag="VN778_01255"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:NF013468.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HXR49635.1"
                     /translation="MFDEAITKLKQPGAVGVIPTDTVYGVVARAADEAAVARLYELKK
                     RENKPGTIIAASIDQLVELGIKRRYLTAAEQFWPGAISVEIPHHITYLHQGTGRQAFR
                     ITDDKELLKLLEQTGPLQTSSANQPGEPPANTVAEARNYFGNKVDFYIDGGDLSDREP
                     STVIRIVDDAIEIIREGAVKLDEAGRKLS"
     gene            complement(3024..3254)
                     /locus_tag="VN778_01260"
     CDS             complement(3024..3254)
                     /locus_tag="VN778_01260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49636.1"
                     /translation="MEPKPAPPPEAENPTPASPAPIMDVAPPPQAENGDAPAQQFRLP
                     KKLNLKLKNQMKKPLNRRLNPSHPGNTATASA"
     gene            complement(3290..4111)
                     /locus_tag="VN778_01265"
     CDS             complement(3290..4111)
                     /locus_tag="VN778_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006188781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HXR49637.1"
                     /translation="MINDTAIEDFRATVWGYYRDHGRHDLPWRFPEASGQFDPYKILV
                     SEVMLQQTQVARVIPKYQEFLRRFPSVQALAGAGLGDVLRAWNGLGYNRRAKFLHQAA
                     KCIANEYSGSLPQEVSQLVKLPGIGVNTAGAVVAYAFNQPIAFLETNIRTVFIYHFFA
                     DKTAVADQEILHLVNQTVGPENPREWYWALMDYGSYLKQTTGNLNKLSKHYSKQSKFE
                     GSKRQIRGHVLRLLGTQSLTYEQLIQQITDPRLQEVVDDLLREGLITRKGQALTL"
BASE COUNT          983 a         1081 c         1025 g         1064 t
ORIGIN      
        1 gtggtgacat aagcttgcag tttttgtagc gtgcctttga tatcgccatt gttcttatcg
       61 gcagcgtaga cggcggtccg cagcctgatc atgtgctcat cgttagccct cagggcgaat
      121 aggcaaatgg cggtactgac gatggtcagc gctagaaaat accacgggct gacttggcgc
      181 actttggtcc aaatgtgatg caggcgtttt ttgttcatcc gttaattata tctccctgga
      241 cgcccgctag cctaggctgt accctataag catgcatatt tacttctcag ggattggtgg
      301 ggcagggata ggaccattgg cattgatcgc gcaccaggcc ggctatacgg tttcgggctc
      361 ggacaaacaa gattcacagc atatccaata tttgaaaaga catggtattg cgaacatcca
      421 cattggacaa accgaagcgg caattgctga agtgtacgag cgccagccta tcgattggtt
      481 tgtctacact tctgccctgc ctctggagaa tcctgacgcc ccggaactgg cttactgcga
      541 cgagcaggac atcaagacga gcaagcgaga tgagctccta aaccagattt tgcgcgaccg
      601 gcagctcaaa ctgatcgcta tcgccggtac tcacggcaaa acaacgacga cggctataac
      661 aacctggtta ttcaagcagc tcggcttacc ggttagctat ttactgcccg ccaagaccag
      721 ctttgccgag atgggcgaat ttaacccgaa gggcgaatat tttatctacg aagccgatga
      781 gtttgaccgc aatttcttgt cttatgagcc ggccatcagc ttaatcactg gggtcagctg
      841 ggaccaccac gagctcttcc cgacacgcga ggactaccaa gcagcttttc gggagttcat
      901 cgaccagagc caacgtacgt atatctggca agaagatgtg gactacttgg ggctagaagt
      961 tgcgacagat gtgcacgtac taaacagtgc ttccccaaaa attgaaagcc tcaaactgct
     1021 tggcaagtac aaccgcctgg acgcctggtt agccatacaa gccgtgcata gcgtcaccgg
     1081 caagcctgta gaacaactcg ttgatctcgc taatcggttc cctggccttt cccgccgcat
     1141 ggagcaaatc attcccaatc tgtacagcga ttacgcacac acccctgaaa agattcgcgg
     1201 ggccatgagc gtcgctctgg aaatggctgc cgaaagcggt caaaatgttg tggttgtcta
     1261 cgagccgctg actaatcggc gccagcacta catgcttgat gaataccggg attgctttgc
     1321 cggcgccagc aaactctatt ggatcccgag ctatctggcc cgcgaagatc cggcgcagcg
     1381 catcattccc ccgtctgagc tgatctcaca tctttcagat ccttcgatcg ccgaacccat
     1441 ggaacgcgac gccgcactca aaaagaccat ccaaaaacac ctagaccacg gcgacatggt
     1501 cgtcggtatg gccggtggtg gtggcaacag cctcgacgat tggctccgcg aacaatttaa
     1561 atcctagagt gcgttactta ttccagccgt actcggcgta agaactgccc gccaaatatt
     1621 gctcgatgta aaaacgggca gaaggcacca tattttctgg taattggttc gggtcaaacc
     1681 agaccagctc tccgtgtttt tctggttcgg cattataaag ctcacctccc cacttcagga
     1741 cctcaaaaac aaggtcaacc catacagaac caggttcatt gcgatggata attagcacgg
     1801 gctttaagtc ttgcggttga atagttgcac cagcttcctc gtatgcttct cgaattgccg
     1861 accgggtaaa gggctcgcct ttatccactc ttcccgccac taaactgtag tgtccattca
     1921 tccaccccgt attgctccgt agtaaaaagg caattttgcc gtctttttta aaaattagat
     1981 acactgcggt atacggcgtt tcaacgccgt cgtgccgtcc gctcatcgat tgtccccttc
     2041 tccgccgagg acgccgcggg cctggcggct cttaagcttg gcgatatttt tggcggcgat
     2101 ttcatcaaaa gaaaaaccaa ggtcatgagc gcaaacggca agataccaga gtacatcacc
     2161 aatttctttg gctatttcct gcttgtcttc gtcatccaac ttaccgtgct tgtcgcgaac
     2221 gattttcttg agtttttcgg ccacttcgcc ggcttcgccc gtaatgccta gcacccagtg
     2281 caacaaatct ttaaattcat cacccgtggt taaaacggtg gtcatggcct gcttttgata
     2341 ctcatcgaat gtcatgataa cttcctccct gcttcatcta gtttaacagc gccctcacga
     2401 ataatctcga ttgcatcgtc gacgattcga atgaccgtcg atggttcgcg gtccgaaaga
     2461 tcaccgccat cgatgtagaa atcaaccttg ttaccgaaat aattgcgagc ttcggcaacg
     2521 gtattggctg gcggttcacc tggctggtta gcactactcg tctgtaaagg ccctgtttgc
     2581 tctaataatt tcagtagctc tttatcgtcg gtaatgcgaa atgcttggcg gcccgtgcct
     2641 tgatgaagat atgtaatatg gtgtggaatt tcaacgctga tggcccctgg ccagaattgc
     2701 tcggcggccg tgagatagcg gcgcttgatg ccaagctcaa ctagctggtc gatgctggcg
     2761 gcaatgatgg ttcccggctt attttctcgt ttcttgagtt catacagacg ggccacggct
     2821 gcttcatcgg cggctcgtgc taccactcca taaacagtat ccgtgggaat aacgccaaca
     2881 gcaccgggct gcttcagttt tgtaatagcc tcgtcaaaca cacctctagt ttaacaaatg
     2941 gcggagctac ttctgggttt tgatgtaagc gtatgtagcc agagctgcca ggccgagaat
     3001 gatgatgacg gtcgcgatga tagctaagcc gacgccgttg ccgtgttgcc tgggtggctt
     3061 gggttgagtc ggcggtttag aggctttttc atctgatttt ttagcttcag gtttagcttc
     3121 ttcggaagcc ggaactgctg agcaggtgca tcgccgtttt cggcttgagg cggtggtgcc
     3181 acgtccatga taggtgccgg cgaagcggga gtcggatttt cggcctcagg tggtggcgca
     3241 ggttttggtt ccataatgta attcaaattg tatcacggaa gcataagccc tataaagtta
     3301 gggcctggcc tttccgggta atgagacctt cgcgtagcaa gtcatcaacc acttcttgaa
     3361 gtcgagggtc agtaatttgt tggatcaact gctcgtaagt caaagattgc gtccctaata
     3421 aacggagcac gtggccgcgg atttgtcgct ttgaaccttc aaattttgac tgcttggagt
     3481 agtgcttact taacttgtta agatttccgg tggtttgctt caaatagctg ccgtaatcca
     3541 tgagcgccca gtaccattca cgagggtttt ctgggcctac agtctggttt actagatgca
     3601 gaatttcttg gtcagcaaca gctgttttgt ctgcaaaaaa atgataaatg aacacggtgc
     3661 ggatattcgt ttccagaaaa gcaattggct gattgaaagc gtaggccact actgcgccgg
     3721 cagtattcac gccgatacca ggcagcttca ccagctgact aacctcttgc gggagacttc
     3781 cactatattc gttagcaata catttcgcag cctggtgcag aaactttgcc cggcggttat
     3841 aacctaaccc attccacgct cgaagcacat cgccaagccc ggcgccagct agtgcttgaa
     3901 ctgagggaaa gcgccgtaaa aactcctggt attttgggat cactcgggca acttgtgtct
     3961 gttgcagcat aacttcgctg accaaaattt tatacggatc aaactgcccg ctagcttcag
     4021 ggaagcgcca aggcaaatcg tgccgcccat gatcccgata atatccccaa accgttgccc
     4081 ggaaatcctc tatagcggtg tcgttaatca ttacgattca ttgtattata agaggaagta
     4141 tatgcttcaa ttc
//
LOCUS       DATNGP010000051         2533 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_4415595, whole genome shotgun sequence.
ACCESSION   DATNGP010000051 DATNGP010000000
VERSION     DATNGP010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2533
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4415595"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(490..1413)
                     /locus_tag="VN778_01270"
     CDS             complement(490..1413)
                     /locus_tag="VN778_01270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49638.1"
                     /translation="MAFLKKHSKSSSPPSRQVTMLGRTMPYSYHARRSSEPRALGRQL
                     LSSAVNARHAKKVAHYWRQRFGLFLIILAILFGVANLLTLDTSPVVKPLSGSGAVAFL
                     HSLPSYQRTAERLFGSSLLNHNKITVDTASVERGLKKAYPELSVVSVTLPLFGHRPYI
                     YISQADPALVLRTQTNQRFIIDTQGRAIAATNVSASSDLLEVRDQSGSTIHLGDLALP
                     STTVGFIRAVHFQLAQRQAHVSYVTLPPSGSELDVYMAGTQYFVKFNLASGTALQQAG
                     TFLATQQYFEERGIDPSQYVDVRVDGRAYYR"
     gene            complement(1450..>2533)
                     /locus_tag="VN778_01275"
     CDS             complement(1450..>2533)
                     /locus_tag="VN778_01275"
                     /inference="COORDINATES: protein motif:HMM:NF016027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HXR49639.1"
                     /translation="KKLRPDIRLVYISEKGGVLTDVLTGHAAIDATYSVRAGKFRRYK
                     VDGWRQAFNVPMQLLNVRDAIRLLIGIWQSYWLIRKLHPGVIFTRGGYVSVPVALGGK
                     LNGVPFVTHDSDSTIGLANRIIGRWAAVHAVGMDPTLYPYPKGSSVLVGVPASSEYER
                     VTTKLQHSYRHDLGLRPDGKLLFVTGGGNGASSLNQLVAHSAQKLLTRYPDLTIIHAS
                     GRSLETALNQLYDKLLDSGLRRRVLVKGFVLDMYRYTGAADVVIARAGASTLAELAIQ
                     GKACVVIPASHLTWQVHQARTLAEHQAIMYLPESKAAVPNRLEEVVSDLLDDASKRAE
                     LGNAMHSLAIPDAALRLAVLLLDQLR"
BASE COUNT          599 a          687 c          651 g          596 t
ORIGIN      
        1 gaacgtcgca caatgtatat tttgcgtctc actataaggc atgtgagatg gttaacgata
       61 ccactgcctg caggggactt tatggtgcct aggctacaaa actgcaattt ggcttatgct
      121 gtatatacac ggcagactcc gtactatagc gccccggccg cctatcgcag cgaaacacac
      181 cgcaatgctg ataaatttgc atagatttgc attatgtgat gtttgtgata attcatacat
      241 ttgacttttc cacagcttat gtcgtgaaaa cttgcttttg tacttgctta atgagctgat
      301 atatgcccct ttccccttgt ctctactgct ctattttttg acattgccct gctttattgc
      361 agtagtatag ttgttttaat tccttgctaa aaattgactt taaacatttt acgaacaagg
      421 ggcagagcgg cagggctcac ttgtcgtcat ccatatatct cgctactacg gagcatgagt
      481 tgagtcgcct tagcggtaat aggcacggcc gtcgacgcgt acgtcaacat actggctggg
      541 gtcaatcccc cgctcttcaa agtactgctg ggtcgccaaa aaagtgcctg cctgctgcaa
      601 ggcggtgccg ctcgctaagt tgaacttaac aaaatactga gtgcccgcca tatagacatc
      661 gagctcacta cctgagggcg gcagcgttac atagctgaca tgagcttggc gctgggctaa
      721 ttgaaaatgg acagcccgaa taaagccaac tgtggtacta ggcaacgcca gatcgcccaa
      781 atgaatcgta gagccgctct ggtcacgaac ctctagtaag tcacttgagg ccgagacgtt
      841 cgtggcggcg atggcgcggc cctgcgtatc gataataaaa cgctggttag tttgagtgcg
      901 cagcacaagc gctggatcgg cttgcgagat ataaatgtac gggcgatggc caaacagcgg
      961 caacgttacc gagacaaccg atagctcggg ataggccttc ttcaatccac gctccaccga
     1021 tgccgtatcg acagtaatct tattgtgatt gagaagcgag ctgccaaata gccgctcggc
     1081 cgtccgctgg tagctcggca gcgaatgcag aaacgccacc gcgcccgatc cgctaagcgg
     1141 tttaacaacc ggactagtat cgagtgttaa cagattagca acaccgaaaa gaatagccag
     1201 tatgatcaaa aataatccga accgctgccg ccagtagtga gcaacctttt tagcgtggcg
     1261 ggcgtttaca gcgctgctga gcagctgtct tcccagcgcc cgcggttcac tcgaacgccg
     1321 ggcgtgatag gagtatggca ttgtccgtcc cagcattgta acctggcgcg acggaggtga
     1381 tgacgatttg gaatgttttt tcagaaatgc catgattatt tattttggag ccgcccaggg
     1441 tgacaatatt tatcgcaact ggtctaataa tagcaccgcg aggcgcagcg cggcatcggg
     1501 aatcgccagg gagtgcatgg cattgccgag ttcggcacgc tttgacgcat catcgagcag
     1561 atcgcttaca acttcctcta gccgattcgg taccgcagcc ttactctcgg gcaggtacat
     1621 aattgcctgg tgctccgcca gggtacgcgc ctgatgcact tgccacgtca ggtgactggc
     1681 tggaataacg acacaagcct tgccctggat tgccagttct gccagcgtac tggcgcccgc
     1741 tcgagctatg acgacatccg cagcaccggt gtaacgatac atgtccagca cgaagccctt
     1801 cacgagcaca cgacggcgaa gtccgctatc gagcaatttg tcgtagagct ggtttaatgc
     1861 cgtctccagc gaccgcccgg aggcatgaat aatcgtcaga tccgggtaac gcgtcaggag
     1921 cttctgcgcg ctatgcgcaa cgagctgatt taacgaactg gctccgttcc cgccgcccgt
     1981 cacaaataac agcttgccgt caggtcgcaa gcccagatcg tggcgatagg aatgttgtaa
     2041 ctttgtcgtt accctttcgt actcgctgct cgccgggacc cctacgagta cgctgctacc
     2101 ctttggatag ggatataacg tagggtccat gccgactgca tggaccgccg cccaacgacc
     2161 gatgatacgg ttggctagac cgatggtact atctgaatcg tgggtcacga aaggaacgcc
     2221 attcagcttg ccacctaacg cgaccggtac gctgacgtac cctcccctgg tgaatatcac
     2281 tcccgggtga agttttcgta tcagccagta actctgccag atgccaatta gtagccgtat
     2341 agcatcacgc acattgagta gctgcatcgg cacgttgaag gcctgccgcc agccatcaac
     2401 cttgtagcgc cggaacttcc ctgctcgcac gctgtaggta gcatcaatag cggcgtggcc
     2461 ggtaagtaca tcggttaaca cgccgccctt ctcgctgata tacaccaagc gaatatccgg
     2521 acgaagcttt ttt
//
LOCUS       DATNGP010000052         4209 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_1869737, whole genome shotgun sequence.
ACCESSION   DATNGP010000052 DATNGP010000000
VERSION     DATNGP010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4209
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1869737"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..698
                     /locus_tag="VN778_01280"
     CDS             <1..698
                     /locus_tag="VN778_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXR49640.1"
                     /translation="FGAESAMIRLDMSEYQRPDDVQRLLATGEHEAASLLMSVRNQPF
                     SVVLLDEIEKAHPNILNLLLQLLDEGQLTDTSGRVASFKDCIIIATSNAGAQTIRERV
                     GKGESLESFSSEFSDELINSGQFKPELLNRFDEMVLFRPLNADELAQVVHLLIAEVNK
                     TLANQNISIELTPAAIQAIVSAGNDPRLGARPMRRVLQRAVEDTVAQKILRGEAKPGD
                     HLLLDTPDLALAS"
     gene            complement(691..1407)
                     /gene="pyrH"
                     /locus_tag="VN778_01285"
     CDS             complement(691..1407)
                     /gene="pyrH"
                     /locus_tag="VN778_01285"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010164214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HXR49641.1"
                     /translation="MSSSVYKRVLLKLSGEQLAGQYEFGVDPEIASFLAAEVKKAVDA
                     GCQIVMIVGGGNMVRGAEIAGHGIRRVTADQMGMLSGLINSIALTDIFEAHDVVTRCL
                     SNIFAQQIAESYSYRLAEKHLQKGRVVIVAGGTARPYVTHDTAAVSFALELDCELVLK
                     ATKFDGVYDKDPAEHPDAVKLNEVDFQNAIENSAIKVMDKAALGLAMEQKKKLVVFDA
                     MQPDNLVKIVRGEPVGTLIS"
     gene            1460..2257
                     /locus_tag="VN778_01290"
     CDS             1460..2257
                     /locus_tag="VN778_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HXR49642.1"
                     /translation="MKLYSWNVNGIRAVTKKGLFQPFLDKEKPDILCLQEIKAKPEQI
                     ELELDGYTAFWYSAEKPGYSGTALLSKIPPKQVINGFPQGIIEEFKVTGDIYGDPNEE
                     GRVIAAEFDKFWAVTVYTPNAKDDLSRIPLRHKHWDPAFLAYCKQLEQSKPVVFCGDL
                     NVAHTELDLANPKPNRGKKGFTDEERSGFQAFLDSGFVDTLRMFHEGNGYYTWWSHFA
                     NARARNVGWRIDYVLVSHDLKSKVKAAEIHPKVMGSDHCPVSVTLDL"
     gene            2254..3177
                     /locus_tag="VN778_01295"
     CDS             2254..3177
                     /locus_tag="VN778_01295"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXR49643.1"
                     /translation="MKLEFVSFLATDKVKLPGLLYTPQSSTKKAAVWLHGMGDNGVFY
                     NPKRMNALGEALTEKGIAFLAFNNRGAHNSKGLSIDDESLPEEDRRYPGGTYYEKVAD
                     CVHDIDGAVHFLKACGFSELYLLGHSTGANKICAYHVRVSKNPFSKYVLAGPGDDTGL
                     FFTDLGPKRFWQAVQYAARAVSEGDPLKTMPKYTGMYPFSAQSAWDILNPDGDYNTFP
                     YYEFTTERLGQKQLFEEYQKINRPTLVIIGEHDEYMTTAGSAAGALEILLKHTPNAMM
                     KHHDFTLLPGADHSFHDSEQAFAEQVAGWLV"
     gene            3178..3966
                     /locus_tag="VN778_01300"
     CDS             3178..3966
                     /locus_tag="VN778_01300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49644.1"
                     /translation="MDREFWHKQTPGQPLFADLLWSRPENKRQAGKLLIVGGNAHGFA
                     APAQAYAEAEKAGVGTARVLLPDSLQKTVGRVLEAGEYAPSTPSGSFSRQAVSEALAM
                     GNWADGVLLAGDFGRNSETAVLLEQLIAKHDGQLTITQDALDYFTKSPLALLDREKTT
                     VVASFAQLQKIASSAHFPTAFTFDMDFLRLIDVVHELSLQHSAAIVTKHLETIFVAYN
                     GEVGTTKLKEDKPIWRVETAAHAAVWWLQNPAKQFESLTTSLIS"
     gene            complement(3971..4057)
                     /locus_tag="VN778_01305"
     tRNA            complement(3971..4057)
                     /locus_tag="VN778_01305"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4021..4023),aa:Leu,seq:caa)
BASE COUNT         1055 a         1128 c         1043 g          983 t
ORIGIN      
        1 acttcggcgc cgaaagtgcc atgatccgcc tcgacatgag cgaataccaa cgtcctgatg
       61 atgtgcaacg cctgctggca acgggtgaac atgaagctgc tagcttgctg atgtcggtgc
      121 gcaatcaacc attttccgtg gtgctactgg atgagattga aaaagcccat cccaacatcc
      181 tgaatttatt acttcagctg ctcgatgaag ggcaactgac cgatacatcc ggacgagttg
      241 cttctttcaa agactgcatt atcattgcca cctcaaatgc cggagcgcag acaattcgcg
      301 aacgcgtagg caaaggcgag agcctggaga gtttttctag tgaattctca gacgaactca
      361 tcaatagtgg ccagttcaag ccggaactgc tcaaccgctt cgacgaaatg gtcctcttcc
      421 gtcccctgaa tgccgacgaa ctagcccagg tcgtgcattt actcatagcc gaagtcaaca
      481 aaactttggc caatcaaaat atctcaatcg aattgacgcc ggctgccatc caggccatcg
      541 tcagcgccgg caatgatcca cgcctggggg ctcgccctat gcgccgggtc ttgcagcgtg
      601 ctgtcgaaga cacagtcgcc caaaaaatcc tccgcggcga agctaagccc ggcgatcatc
      661 tgctactcga caccccagat ctggctttag ctagctaatc agcgtgccga ccggttcgcc
      721 acgaacgatt ttcaccaaat tgtcaggttg catggcgtca aagactacca gtttcttttt
      781 ttgctccatg gcgaggccta aagcggcttt atccataact ttaatggcac tgttttcgat
      841 cgcgttttgg aagtcgactt cattcagttt tacggcgtcg ggatgctcgg cggggtcttt
      901 atcgtacacg ccatcgaact tcgtggcttt caataccagc tcacaatcca actcgagagc
      961 aaaactgacc gccgcagtat cgtgtgtaac atagggtcgg gccgtgccgc cggcgacaat
     1021 gaccactcgg cctttttgca agtgcttttc cgccagtcga taggagtatg attcggcaat
     1081 ttgctgtgcg aatatgttac ttaaacagcg cgtcacgacg tcatgggctt caaaaatatc
     1141 ggttagcgca atggagttta tcaggccgct caacatgccc atctgatcag ccgtgacccg
     1201 acgaatgcca tgtccggcga tttccgcacc gcggaccatg ttgccgccgc cgacgatcat
     1261 cactatttga catccggcat ccactgcctt tttaacttcg gctgctaaga aagaagctat
     1321 ttcgggatcc acgccaaact cgtattggcc ggctaattgc tcgcccgaaa gcttgagcag
     1381 tactcgctta taaacgctag aactcatcta agtcgttagt ctagcaaata atcactgtga
     1441 gagctatact tgcccaatga tcaagttata ctcctggaac gttaatggca tccgcgccgt
     1501 taccaaaaaa ggccttttcc agccgttttt agacaaagaa aaaccggaca tcctctgctt
     1561 gcaagagatc aaagccaagc ccgagcaaat cgagttggag ctcgacggtt acacggcctt
     1621 ttggtactcc gcagagaagc cgggttattc cggcaccgcg ctactgagta aaatcccgcc
     1681 aaaacaggtg attaacggct tcccacaggg aattatcgag gaatttaaag tcaccggtga
     1741 catctacggc gatccgaacg aagagggcag ggtgattgcc gccgagtttg ataagttctg
     1801 ggctgtcacg gtctacacgc caaatgccaa agacgacctc agccgcatcc cgctgcgcca
     1861 taagcattgg gatccggcct ttttggctta ttgcaagcag ctcgagcagt ctaagccagt
     1921 cgtcttttgt ggcgacctca atgtcgccca caccgagctt gacctagcca atcccaaacc
     1981 caatcggggc aaaaaaggct tcaccgacga ggagcgatcc ggtttccaag ctttcctaga
     2041 cagtggtttc gttgacacac tgcgcatgtt tcatgaaggc aatggttact acacgtggtg
     2101 gtcccacttc gccaacgccc gcgcccgcaa cgtcggctgg cgcatcgact acgtcctcgt
     2161 ttcccacgac ctgaaaagta aggtcaaagc tgccgagatc caccccaaag tcatgggctc
     2221 cgaccactgt ccggtatctg ttacactaga cttgtgaagt tagagtttgt ttcatttttg
     2281 gccaccgata aggtgaagtt gcctggattg ctgtatacac cgcagtcgtc aaccaaaaaa
     2341 gcggcggttt ggctgcacgg catgggtgat aatggcgtgt tttacaaccc gaaaagaatg
     2401 aatgctcttg gagaagctct tacagagaag ggcatcgcct ttttagcctt caataatcgg
     2461 ggcgcacata actctaaagg tctcagtatt gacgacgaaa gtttgccaga ggaagacaga
     2521 cgctatccgg ggggcacata ctatgaaaag gttgccgatt gtgttcatga tatcgatggg
     2581 gccgtccact tcttgaaagc ctgtggcttt agtgaacttt atttgcttgg ccatagcact
     2641 ggcgctaata agatttgtgc gtatcatgtg cgagtcagca aaaatccttt ctcaaagtat
     2701 gtcttggccg gcccaggcga cgatacgggg ctatttttca ccgatctcgg gccaaagcgt
     2761 ttttggcaag cagtgcagta tgccgctcga gctgtcagcg aaggtgaccc gctaaaaacc
     2821 atgccaaaat atactggcat gtacccgttt agcgcccagt cggcttggga tattcttaac
     2881 cccgatggtg actacaacac cttcccatat tacgaattta caactgaacg gcttggccaa
     2941 aagcagctgt ttgaagagta tcaaaagatc aaccgtccga cactagtaat aatcggcgaa
     3001 cacgatgaat acatgacgac agccggaagc gccgcagggg cacttgaaat cctactgaag
     3061 cacacaccca acgctatgat gaagcatcat gacttcacgc ttttgcctgg agctgaccac
     3121 agttttcacg actccgaaca agcttttgcc gaacaagtag caggctggtt ggtttaaatg
     3181 gaccgggaat tttggcacaa acaaactcct gggcagccgc tttttgcgga tttgctctgg
     3241 agccggccgg agaataagcg gcaggccggc aagctactga ttgtcggtgg caatgcgcac
     3301 ggcttcgccg ccccagctca agcttacgcc gaagctgaaa aagccggcgt aggcaccgcg
     3361 cgtgtattgt tgccggatag cttgcaaaaa acagtcggcc gcgtgctcga agcaggggag
     3421 tacgcgccga gcaccccgag cggcagcttc agccgccaag cagtgagtga agcgctggct
     3481 atgggtaact gggctgatgg cgtcttactg gccggtgatt tcggccgtaa ttctgaaact
     3541 gccgtgctgc ttgagcaact tattgcaaaa cacgatggcc agctgaccat aactcaggat
     3601 gctctggatt atttcacaaa gtcgccactg gctttattgg atcgggaaaa gaccacagtg
     3661 gtagcaagct ttgcccaact gcagaaaatt gctagcagcg cacactttcc gacagctttt
     3721 acctttgata tggacttctt acgtttaata gatgtggtac acgagcttag cttgcagcac
     3781 tcggccgcca ttgtcaccaa gcatctcgag actatcttcg ttgcttacaa tggggaggtc
     3841 ggcactacta agttgaaaga agataaaccg atctggcgag ttgagacggc cgctcatgcc
     3901 gccgtctggt ggctacaaaa tcccgccaaa caatttgaaa gtctaacaac tagtcttatt
     3961 tcttaacctc tggtaccgcg ggccggactt gaaccggcac gtcctagagg acaccagatt
     4021 ttgagtctag cgcgtctacc aattccgccg ccgcggcaca agatgttaaa aaattttatt
     4081 gtcacgcaca tgggcgtagg ctacaagggg taattgtact ataattaaag agttaacgcc
     4141 agtttcctat ggacataatt cctcccactt ctccaagcga tgatcacccg gcaagcacgc
     4201 cggccaatg
//
LOCUS       DATNGP010000053         2351 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_349005, whole genome shotgun sequence.
ACCESSION   DATNGP010000053 DATNGP010000000
VERSION     DATNGP010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2351
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_349005"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(12..416)
                     /locus_tag="VN778_01310"
     CDS             complement(12..416)
                     /locus_tag="VN778_01310"
                     /inference="COORDINATES: protein motif:HMM:NF024143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HXR49645.1"
                     /translation="MAKRMFKNVAIVAALAAAAGFVTGLLTAPKSGKETRADIKNAAK
                     HGLSEAERQLKNLSSELGDLVDQARSKGSELRGKAGETLSQATTQAQDAREKVREMLT
                     AVHDGTASDEDLQLAMNQGRTALKHLRDYLKK"
     gene            complement(425..676)
                     /locus_tag="VN778_01315"
     CDS             complement(425..676)
                     /locus_tag="VN778_01315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49646.1"
                     /translation="MQNAESILVIIVSTVLAIFLVVLVVVLVLIAKLVQALRRMVERA
                     EEVVETAGEAASLLRNASGPLALFKVVRNIMKAVEKARK"
     gene            792..1919
                     /locus_tag="VN778_01320"
     CDS             792..1919
                     /locus_tag="VN778_01320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49647.1"
                     /translation="MTRGNFSESSLDAHDADAAKQLGERQGWQSRVGAPVFGALTCDV
                     YPQLVSGVTDVQIATVLLTPGALQFELLECDESRRASLMFLQQASWVLGLPISLDLTP
                     VDRNDFNEINQRRLEHEAASISAIEIALGRESLERLTSTAPDGASFQAACQLYTNFRD
                     HLKAFKEGLVAETVVPINLLVAAILTMPPGFEPIVQSLEDVARIYSEPWFQNLMVQAS
                     STAGGYWRRVSTHIVFNDYQAEVLTQLALRNGNLGFSFDRAPDCKTRFRFTKEVQSRL
                     MQHWRQHNENAARTYASGDTTNEHFVEGSSDACPARKVSFSVPDAGAAHYVASLLGVD
                     EARLFAGHPKSPIAIGMDCLAGLLYRAAQVVTSPDASFPAV"
     gene            complement(1916..>2351)
                     /locus_tag="VN778_01325"
     CDS             complement(1916..>2351)
                     /locus_tag="VN778_01325"
                     /inference="COORDINATES: protein motif:HMM:NF014674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     GatCAB subunit B"
                     /protein_id="HXR49648.1"
                     /translation="KALVHATPEDARRIAFWLMRPDAEDAEQTPERKIDIDELITLSK
                     LTGEGKLSSSAAKQVLDVLLATGGTAENIANELNLIQVSDEGTIAKIVEQVIAENPKA
                     VEDLKAGQERAIGFLVGQIMKLSQGKANPAMAQSLIRKQLGL"
BASE COUNT          510 a          668 c          600 g          573 t
ORIGIN      
        1 attttaccgt actacttttt taagtagtca cggaggtgct tgagggcggt gcggccctgg
       61 ttcatggcga gctgaaggtc ttcgtcgctg gccgtaccat catgtacagc cgtcaacatt
      121 tcgcggactt tctcgcgcgc atcctgagcc tgagtagttg cctgagacaa agtttcgcct
      181 gctttgccgc ggagttcact acccttggat ctggcctggt cgacaaggtc gccaagctcg
      241 ctgctcaaat tcttgagctg ccgctccgcc tcactcaagc cgtgtttggc agcattcttt
      301 atatccgcgc gtgtctcttt gcccgatttc ggggccgtca agaggccggt cacaaaccca
      361 gctgctgcag caagcgccgc tacaatcgct acgtttttaa acattcgctt tgccatagtt
      421 cctcctactt tcgcgccttt tcgacggctt tcataatatt acgaacaacc ttaaagagcg
      481 ccagtggccc gctggcattc cggagcagcg atgcggcttc accggccgtc tcgaccacct
      541 cttcggctcg ctctaccatt cgccggagcg cctggactaa tttagcgatc agcaccagca
      601 cgactaccag aacaaccaga aaaatggcga gtactgtcga aacaataatg accaggattg
      661 attcagcatt ttgcatagta ttagttaata ccagctggcc gtttttcgca attatctgag
      721 cagcctacgt actatctcta gagatctgtc tctagacatg gtgaacaatt tatagtatag
      781 tttatatact catgacgcgc ggcaatttct ctgaatcctc actagatgct cacgatgcag
      841 acgccgccaa acaactcggc gaacgccaag gttggcagtc tcgagtggga gcgccagttt
      901 tcggcgcgct aacgtgcgac gtatacccgc agctcgtctc aggcgtcaca gacgtacaga
      961 ttgccacggt ccttcttacg ccaggtgcct tacagttcga gctgcttgag tgcgacgaat
     1021 cccgacgcgc ttcgctcatg ttcttacaac aagcatcttg ggtccttggc ttgccgatat
     1081 cgcttgatct tacccctgtc gatcgcaatg acttcaacga aatcaatcag cgacgtctcg
     1141 agcacgaagc agcatcaatc agtgcaatcg agatcgcact tggccgcgag tcgctcgagc
     1201 gcctaacgtc taccgcaccc gacggcgctt cattccaggc tgcatgccag ctatacacga
     1261 actttcgtga tcatcttaaa gccttcaaag aaggtttggt agccgagacg gttgtaccca
     1321 tcaatctgct cgtagctgca atcttgacga tgcctcccgg gtttgagcct atcgtgcagt
     1381 cactagagga cgtcgcgcgg atctatagcg aaccctggtt ccagaaccta atggttcagg
     1441 cgtcgagtac cgctggtgga tattggcgaa gggtgagcac acacatcgta ttcaacgact
     1501 accaggcaga ggtactgaca caactggcct tacgaaacgg taatctcggc tttagcttcg
     1561 atcgagcccc cgactgcaag accaggttcc gttttacaaa agaagtacag tcgaggctca
     1621 tgcaacactg gcggcagcac aacgagaatg cagcccgaac ttacgcgagt ggagacacca
     1681 cgaatgagca ctttgtggaa ggcagctcag atgcatgccc agcacggaaa gtatcgttct
     1741 ccgttcctga tgcgggcgct gctcattacg tagctagctt gctaggcgta gatgaagcgc
     1801 ggctgttcgc cgggcaccct aaaagcccga tcgctatagg catggattgc cttgctgggt
     1861 tgctgtatcg tgcggcgcaa gtcgtcactt caccagacgc ttcgtttccg gcagtttaga
     1921 gaccgagctg cttgcggatg agtgattgcg ccatggcagg gttggccttg ccttggctca
     1981 gcttcataat ttgaccgaca aggaagccta tcgcgcgctc ctgacctgcc ttcagatctt
     2041 ctaccgcttt aggattctcg gcaataacct gctcaacgat cttggcaatc gtgccttcat
     2101 ctgacacctg gataaggttc agctcgtttg caatgttttc agcggtgccg cctgtggcta
     2161 gcaacacgtc gaggacctgc ttggcagccg aagaactcag cttcccctcg ccggtaagtt
     2221 tagacaacgt aatgagctca tcgatgtcga tcttgcgctc gggcgtctgc tcagcatctt
     2281 cagcgtctgg acgcatgagc cagaatgcga tacgccgggc gtcttcgggt gtagcatgta
     2341 cgagcgcttt g
//
LOCUS       DATNGP010000054         2446 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_2687924, whole genome shotgun sequence.
ACCESSION   DATNGP010000054 DATNGP010000000
VERSION     DATNGP010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2446
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2687924"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..415)
                     /locus_tag="VN778_01330"
     CDS             complement(<1..415)
                     /locus_tag="VN778_01330"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HXR49649.1"
                     /translation="MSQWATIVDIAAKVQAGELKAADLVEQSLAAIGEKKEYDAIIAT
                     AEARARERAHGIDARIAKGETVGKLAGVPFIAKDNFLVFGAETTAASNILRGFDTPYQ
                     ATAIEKLEAEGAICVAKANLDAFAHGSSTENSDFFT"
     gene            complement(412..705)
                     /gene="gatC"
                     /locus_tag="VN778_01335"
     CDS             complement(412..705)
                     /gene="gatC"
                     /locus_tag="VN778_01335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HXR49650.1"
                     /translation="MAKLTRDDVLKLALLARLELSDTEIEEFADELSEILQYVEQLQN
                     VDVSGLQPTNQVTGLTNVTRDDIVRGYGYEPKELLKNVPTVDNDQIKVRRMIG"
     gene            876..2057
                     /locus_tag="VN778_01340"
     CDS             876..2057
                     /locus_tag="VN778_01340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXR49651.1"
                     /translation="MSALAHPLEVRAPAAEPQVDYEIGTPAQLYDFPSPAPENPCSLE
                     ALAHFTLAHASAGARFWGRAASVALRRSFHNTPENGRPAPERMVAPLKKHQHEFLVSQ
                     PHPRSGIKSSEAATIVLLPGLSEIDRGSALRLHLAIANHPGFDTRRVVTKCTEGVSHD
                     GQLLPLNTGHRRNSREMATDLLQVLPRVTNDGPTEICGTSWGSFLGVETVDQNLAANP
                     KDQRNITRLTLLASAVIAREVDYESLGVERYFPPEMSDDEVRDRLTHDFFNHIPHDLY
                     SMYLKHPEDLLECTEAVFSYFLQPHKALHRLSAIYGNFRNVQKGIEVEAVERIASTHQ
                     LWAIGGESDPLVRAQIPFWTAYEKRYPLHVRQKIIPGMGHLMTANAAGIAAELAAMEA
                     A"
BASE COUNT          624 a          619 c          559 g          644 t
ORIGIN      
        1 tcgtgaagaa atcactattt tcggtgctgg agccatgggc aaaagcatca aggttagcct
       61 tggccacgca gatggcgccc tcggcttcca acttttcaat ggccgtcgct tgatacggcg
      121 tatcaaaccc gcgtaagata ttgctggcag cagtcgtttc cgcgccaaat accaagaagt
      181 tatctttggc gataaaaggc acgccagcca atttgccaac cgtctctcct tttgcaatcc
      241 gagcatcaat accatgtgct cgttcgcgtg ctcgagcttc ggccgtggca atgatagcat
      301 cgtattcttt cttttcaccg atagccgcaa gcgactgttc cacgagatca gcagctttaa
      361 gttcacccgc ctggactttt gcagcaatat ctacaattgt cgcccactgg ctcatccgat
      421 catccttcgg actttaatct gatcgttgtc gaccgtaggc acatttttta gcaattcttt
      481 aggctcatag ccgtagccac ggactatatc atctcgcgtt acgttggtga ggcccgtcac
      541 ttgattagtc ggctggagac cacttacatc gacattctgt agctgttcaa cgtactggag
      601 aatctctgac agctcgtcgg caaactcttc aatctccgta tcgctcagct ctaagcgcgc
      661 gagcaaagcc agctttaaga catcatcccg ggtaagtttt gccatttgct agtaagtata
      721 aaagatcacc gcctcatatg ctatacttta cgatttgtga ttaataatat ataactagtt
      781 taattctttc tgtgcttggg caaataaatc cgctttacaa agcactagca taaggattac
      841 gattaggctt agtaagattt tttacagaca tttctatgag cgctctagcc catcccttag
      901 aagtcagagc acctgcagcc gagccacagg tcgattatga aataggcacg ccagcgcaat
      961 tatatgattt tccttcacca gcccctgaaa atccttgcag cttagaggcg cttgctcatt
     1021 ttacattggc tcacgccagt gcgggtgccc gcttttgggg aagagcggca tcagttgctt
     1081 tacggaggag ttttcataat acaccagaaa atggccgccc ggccccggaa cgcatggtcg
     1141 cacctctcaa gaaacaccag catgaatttt tggttagtca accgcacccc cggtcaggaa
     1201 taaaatcaag tgaagctgct accatcgtcc tcttaccagg actatcagaa atagacaggg
     1261 gcagcgctct caggttgcac ttggccatcg ctaatcatcc gggcttcgat actcggaggg
     1321 tagtcaccaa gtgcaccgag ggagttagtc atgacggtca gttactgccc ttaaatactg
     1381 gccatcgccg caattcgaga gagatggcca ctgatctact gcaagttttg ccgagggtta
     1441 ctaatgatgg tcccacggag atttgtggca cctcatgggg cagctttctg ggagtagaga
     1501 ctgttgatca aaacttggcg gcaaatccaa aagatcagcg taatattaca cgtttgacac
     1561 ttttagcttc ggcggtcatt gctcgggaag tagattatga atcgctcggt gttgaaagat
     1621 atttccctcc ggaaatgagc gatgatgaag tccgcgacag gctaactcac gactttttca
     1681 atcacattcc ccacgatcta tacagcatgt atttaaaaca ccctgaggac ttgctcgaat
     1741 gtactgaagc agtcttcagc tactttcttc aaccccacaa agccttacat cggctttcgg
     1801 ctatatatgg caatttccgc aatgtacaaa agggtatcga agtagaggcg gtagagcgga
     1861 tcgccagcac tcaccagcta tgggccatcg gcggcgagag cgacccgtta gttcgagctc
     1921 aaataccttt ttggactgct tacgaaaaac gctaccctct gcatgtgcgg caaaaaatta
     1981 tacctggcat gggacacctt atgacagcta atgctgctgg cattgctgca gagctcgctg
     2041 ctatggaagc cgcctaacgc cccatatccc catctgtgga ttatattaca gccagccact
     2101 cagtttattc tttatactag actttttatc aactgcatgt cgtgcatttg ttggttatgt
     2161 agcctaaatc cttagatagt ccttgaatta gcatgagaat ttaggcaact caataactag
     2221 ccaaggcgta cggcctgttt gctcatggca aaatttgctg ggagggccta tgggtgagtt
     2281 tacaccatct gagttagcac taccgaaaag taaaattgaa atgccaaaaa tagttacggc
     2341 tgctatggct attggaagcg ttgcgctgcc ttcagcggct atcgccgatg gttttacggg
     2401 acatgccaaa gaagcgccgg aacccaaaag tatgccgcca gccatg
//
LOCUS       DATNGP010000055         2249 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_3726670, whole genome shotgun sequence.
ACCESSION   DATNGP010000055 DATNGP010000000
VERSION     DATNGP010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2249
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3726670"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..463
                     /locus_tag="VN778_01345"
     CDS             <1..463
                     /locus_tag="VN778_01345"
                     /inference="COORDINATES: protein motif:HMM:NF012345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HXR49652.1"
                     /translation="YLGLCLGLQMAVIAAARNAGLAPATTFELEADSPELVINTMADQ
                     KDKHMTGGTMRLGDYDCRLEKGSLAAQVYGTTNIVERHRHRGECNNQYRDQYKSWGIR
                     ASGINPENDLVEVIEGLDHPFLLASQYHPEFKSRPNRPHPMFDGFIKALLK"
     gene            576..995
                     /locus_tag="VN778_01350"
     CDS             576..995
                     /locus_tag="VN778_01350"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXR49653.1"
                     /translation="MEPTENTAKSEPAPNNHLKRILLVEDDDGLANVYVSRLQAEGFD
                     VRRVANGEEALAAALSYHPDLVLLDVMMPKVSGFDVLDILRNTPETANLKIIMLTALS
                     QQSDKERAEALGVDDYLVKSQVVIADVVDRIRQHLGE"
     gene            1074..1955
                     /locus_tag="VN778_01355"
     CDS             1074..1955
                     /locus_tag="VN778_01355"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HXR49654.1"
                     /translation="MTKEVLNSQPPHIEQELHRQQASVDLRSARPVIDRAQAVETHGI
                     NGDIMAIISLPGEGVEGKAKEIGVVDYGEPDPANPSPVFIFEGQPLKMFGTARTRYGL
                     WALNYVSEELIGTYTAFPEGAITKLGRASDVADTINASHFLGLSDGSQGNQAISRQHA
                     TIQIKDGQLTVWDHSKNGTKITYANPEATQTSTELAMIQEELGNEALGAAGVVMPENE
                     NAGQKIAELTAHLSEEDQRELRYYASAMGDKRTAQARGAGEDSIYLSQVAGRHYRQLS
                     PEARKIASQYERLFLSE"
     gene            complement(1965..>2249)
                     /locus_tag="VN778_01360"
     CDS             complement(1965..>2249)
                     /locus_tag="VN778_01360"
                     /inference="COORDINATES: protein motif:HMM:NF013203.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HXR49655.1"
                     /translation="QGSSMSHVKAGGTAKNVHDSPGQRLGVKLFGGEKVKTGQVIVRQ
                     VGMTKRPGVGTKMSRNFTIHAARDGVVAYSQRKVRLFSGRSVMRTEVTVQ"
BASE COUNT          592 a          591 c          574 g          492 t
ORIGIN      
        1 gtatctcggc ctatgccttg gcttgcaaat ggccgtgatc gccgctgctc gcaacgctgg
       61 tctcgccccg gcgacgacat tcgaacttga ggctgacagc ccagagctgg tcattaacac
      121 catggccgat caaaaggaca agcacatgac cggcggtacc atgcgccttg gcgactatga
      181 ttgccggcta gaaaagggca gcctggccgc tcaggtctac ggcacaacaa atattgtgga
      241 acgtcatcgt caccgcggcg aatgcaataa tcaataccgt gaccaatata aatcctgggg
      301 catacgcgcg agtggcatca acccggagaa tgacctggtt gaagttattg agggcctcga
      361 tcatccattc ttgctagcca gccagtatca tccagaattc aaaagccgcc caaaccggcc
      421 acacccgatg ttcgacggct tcatcaaagc tcttttgaag tagtccgtgg aagtagtgtt
      481 cacatagtat gggcacgact ccgcagagaa gagtgcaaga ggcttgtcga tagcagtcat
      541 tgaacattcg gctcagatgc gctaccataa gcatcatgga accaaccgaa aacaccgcaa
      601 aatcggaacc cgctcccaac aatcacctaa agcgtattct gttagtagaa gatgacgacg
      661 gcctagcaaa tgtttacgtc tcccgcttgc aagccgaagg cttcgatgtc cgccgcgtcg
      721 caaatggcga agaggcttta gctgccgcgc tgagctacca tcctgatctc gttcttctcg
      781 atgttatgat gcccaaagtc agtggtttcg acgtgctcga tatcttacgc aacacccccg
      841 aaactgccaa cctgaaaatc atcatgctga ccgccctcag ccagcaaagt gacaaagagc
      901 gtgccgaagc cctcggtgtc gacgactacc tcgtcaaatc ccaagtcgtc atcgccgatg
      961 tcgtcgaccg cattcgccag catctcggcg agtaacctca atacttctct cacaatcttg
     1021 acttttcaca aagcttatgc taaagtactt aatgttatta gtaattgcta aagatgacaa
     1081 aagaagtact taattcacaa ccgccacaca tagagcaaga actgcatagg caacaagcta
     1141 gtgtggattt gcgctcagcc aggccggtga tagatcgggc tcaggccgtg gaaactcacg
     1201 gcataaatgg cgatattatg gctataattt ccttgccagg cgagggagta gagggcaaag
     1261 cgaaggaaat cggtgtagtt gattatggcg aaccggatcc agctaacccc agcccagtct
     1321 tcatctttga gggccaaccg cttaagatgt tcggtactgc caggactcgc tatgggttgt
     1381 gggcgctaaa ttacgtttcc gaggagctta taggtacgta cactgctttt cccgaaggtg
     1441 caattacaaa gctagggcgt gcaagtgatg tagctgatac cataaatgcg agtcacttcc
     1501 ttggactgag cgatggcagt cagggcaacc aagcaatttc acgccaacac gccacaatac
     1561 agatcaaaga cggccagttg acggtctggg accattctaa aaacggtact aagatcacct
     1621 acgccaatcc cgaagcgaca cagacatcta ctgaattggc aatgatacaa gaggagctgg
     1681 gcaatgaggc gctcggtgcc gccggtgtcg taatgcccga aaatgaaaat gccggccaaa
     1741 aaatagccga gctcactgct catttaagtg aagaagacca aagggaactc cgatactacg
     1801 cgagcgctat gggagacaag cgtactgctc aggctagagg tgctggcgaa gattctattt
     1861 atttgagtca ggttgctggt cgccactatc gccaactatc gccagaagct cgaaaaatag
     1921 cttctcagta cgaaagatta tttctgagcg agtaaacgta cagcttattg aacggtgact
     1981 tcggtacgca taaccgagcg gccgctgaag aggcggactt tgcgctggct gtaggcaacg
     2041 acgccgtcgc gggcggcgtg gatagtaaag ttgcggctca ttttggtgcc gacacccgga
     2101 cgcttggtca tgccgacctg acgcacgatg acctggccgg ttttgacttt ttcgccacca
     2161 aacaatttaa ccccgaggcg ttggcctgga gagtcgtgga cgtttttggc tgtaccgccg
     2221 gcttttacgt gtgacatgct acttccttg
//
LOCUS       DATNGP010000056         3171 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_1417384, whole genome shotgun sequence.
ACCESSION   DATNGP010000056 DATNGP010000000
VERSION     DATNGP010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3171
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1417384"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..481)
                     /locus_tag="VN778_01365"
     CDS             complement(<1..481)
                     /locus_tag="VN778_01365"
                     /inference="COORDINATES: protein motif:HMM:NF013015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49656.1"
                     /translation="MNAVFALIDCNNFFVSCERLFRPDLEGKPVVVLSSNDGCVVARS
                     NEAKALGIKMAAPAYQSRQLFKEQRVMQFSANFELYGDISDRIVRILTSITPRTEVYS
                     VDESFLDLSLLKIANYQTWGRAIRQRLLREVGVPVSIGIAPTKTLAKLARESAKKEPE
                     "
     gene            complement(484..846)
                     /locus_tag="VN778_01370"
     CDS             complement(484..846)
                     /locus_tag="VN778_01370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49657.1"
                     /translation="MEEDLQDLAGSDGVSIHAGFPNPALDRRGPGPRLSLDLNQLVIH
                     NPSSTYLFRIAGHQWSDQGVYDGDVAVVDRATRRQPSDLVIAWQVSGFVLCRQRQLVH
                     GEETWGVITAIIHEFKRD"
     gene            complement(1299..2729)
                     /gene="gltX"
                     /locus_tag="VN778_01375"
     CDS             complement(1299..2729)
                     /gene="gltX"
                     /locus_tag="VN778_01375"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015642013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HXR49658.1"
                     /translation="MKPVRTRFAPSPTGYLHVGGIRTALFAWLVARQASGKFIVRFED
                     TDKKREVIGAREHLIDSLRQLGLNYDEGPDIGGPFAPYTQSERLDSYKKWAQKLIDTG
                     RAYADPYSPEAVQGFREQAQANKQPFLYRNHRPENPPAWDGQQPLRFIADPKPYEWHD
                     EVMGDLKSGPEVIDDFVLIKSDGYPTYNFAHIVDDAEMEISHIMRGQEFLASVPNYLN
                     LYEALGLNPPVMATLPHVMGPDGKKKLSKRDGAKDVLDYIRDGFLPEALVSFIATLGW
                     NDGTEQEVFTRQELIEKFSLARVQRSGAQFDERRLLWINGSFIRQLALDELFGKVREF
                     WPPEAAEASDDYKKLVLGLVQERLKYFAELPELTSFFFADLPINPELISEHKQLKKLS
                     HDELRSLLEQAKASLESSNFTLDDLTPRLNQLLEQTGQKPATLFSLIRVATTQAPASP
                     GLAETLVVLGKDRSLARLNAQLAAIS"
     gene            complement(2781..>3171)
                     /locus_tag="VN778_01380"
     CDS             complement(2781..>3171)
                     /locus_tag="VN778_01380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49659.1"
                     /translation="LYANLTSIQLQVASSKAGFTANLPSYQPIGYSFAHLNYSPGNVA
                     MQFQSNSDSRAYAISEKLSAWDSQTLRSDFVAGIGQDYQTVQTAGHTLYVYGKNNATW
                     VNSGIWYQIKSDGALSVHQLVELASSM"
BASE COUNT          701 a          816 c          870 g          784 t
ORIGIN      
        1 attccggctc tttcttggcg ctttctcgag ctagtttagc cagtgtttta gtgggcgcaa
       61 tgccgatcga taccggtact cctacttcgc gcagcaaacg ttgccgaata gccctgcccc
      121 atgtttgata attagctatc tttagtaagc tgaggtccag aaatgactca tccaccgaat
      181 atacctccgt tcggggcgtg atgctggtga gtatccgcac aatgcgatca gagatgtcgc
      241 cgtagagctc gaaattagcg ctgaattgca taaccctctg ctccttaaat agttgccggc
      301 tctgataagc cggcgcggcc atcttgatac cgagcgcctt ggcctcgttg gagcgcgcca
      361 ccacacaacc gtcgttgctg gagagcacta ccactggctt gccttccaga tcggggcgaa
      421 aaaggcgttc gcaactgaca aagaagttgt tgcagtcaat gagggcgaag accgcgttca
      481 tagctaatct cgcttgaact catggatgat agcggtgatg acgccccacg tctcttcgcc
      541 gtgcacgagc tggcgctgac ggcaaaggac gaagccactg acctgccaag caatcactaa
      601 atcgctaggc tgccggcgag tagcccgatc gactactgct acatctccgt cgtaaacccc
      661 ttgatcgctc cattgatgtc cggcaatacg gaacagatag gtgctgctag ggttatggat
      721 taccaactga ttgagatcta gcgagagtcg cggtcctggg cctcgtcggt ccagggctgg
      781 gttgggaaaa ccggcgtgaa ttgatactcc atcgcttccg gccaaatctt gaagatcttc
      841 ttccatagtt tcgtctcctt gtgcttcata gtgctcactc acgaatccgc caaaacgtat
      901 tcgcggcctt tgtagactag gcgacgaggg tagctctcaa agcgtaactg gtcgcgatta
      961 ttgctgaaaa aaacagaggt tatttcaaca tctttacgat taaataaaat gttttccatg
     1021 gttcaagctc ctgcttataa gtagtaaaaa agagactgac atcggtgttg ttgccagtga
     1081 acggatttgg agatagaagc ttacagcgag gtgaacgcta ccgttctgag gtcaacccct
     1141 tcggctataa actagtgctt gagcttgaga aagcagggac cgaagtgtgc cttctggtgt
     1201 agataattaa gtccgtgaag taaatccggc tggaagtcct cacgcggcta ctgcttatta
     1261 tacgctgctg tcaataggct gtcacgggcg cagtgtggct agctgatggc ggcgagctgg
     1321 gcgttgaggc gagccagact gcgatctttg ccaaggacta cgagcgtttc agcaagtcct
     1381 gggctggcgg gcgcttgagt agttgctact ctgatgagac tgaagagggt ggctggcttc
     1441 tggccggttt gctctaggag ttggttgagg cgcggagtaa ggtcgtcaag cgtaaaattg
     1501 ctggactcca ggctggcttt agcctgctcc aacaggcttc tgagttcatc gtgggacagt
     1561 ttcttgagct gcttgtgctc gctgatcagc tccggattga ttggcaagtc agcaaagaaa
     1621 aagctggtta gctctggcag ctcggcaaag tacttgaggc gctcttgaac gaggcccagc
     1681 accagctttt tgtagtcatc actggcttcg gctgcttcgg gcggccagaa ttccctcact
     1741 ttgccgaaga gttcatcaag cgccagttgg cggataaaac tgccattgat ccacagcagc
     1801 cgtcgctcat caaattgggc tccgctccgc tgcacgcggg ccagcgaaaa tttttcaatt
     1861 agctcctggc gtgtgaaaac ttcctgctcg gtgccgtcgt tccagccgag cgttgcgata
     1921 aagctgacaa gcgcctccgg taaaaagccg tcgcgaatgt agtccagcac atccttggcg
     1981 ccatcacgtt tagagagttt ctttttgccg tccggcccca taacatgcgg cagtgtagcc
     2041 ataaccggcg ggttgaggcc cagcgcttca tagagattga gatagtttgg cacgctcgcc
     2101 aagaactctt gtccgcgcat gatatggctg atctccattt cggcatcatc gacgatgtgg
     2161 gcaaaattgt acgttggata gccatcggat ttgatgagca caaagtcgtc gatcacctct
     2221 ggtccgctct tgagatcgcc cataacttca tcgtgccatt cgtacggctt cgggtcggct
     2281 atgaagcgca acggctgctg gccatcccag gctggcgggt tctccggccg atgattgcgg
     2341 taaaggaacg gctgcttatt ggcctgcgcc tgttcccgaa acccttgcac cgcttcaggc
     2401 gaatacggat cggcgtaagc ccgcccagta tcaatcagtt tctgtgccca ctttttatag
     2461 ctatctaagc gctcgctttg ggtgtagggt gcgaacggcc cgccaatatc tggcccttca
     2521 tcatagttaa gccccagctg cctaagactg tcgataaggt gctcgcgggc gcctattact
     2581 tctcttttct tgtcggtatc ttcgaaacgc acaatgaatt tcccgctcgc ctgccgcgct
     2641 accagccacg caaacagcgc cgtccgaatc ccgcccacat gcaaataacc cgtcgggctg
     2701 ggagcaaaac gtgttctaac tggcttcata gacctttatt ataagagaaa actcttaaga
     2761 tacgaactgt tgtcggctga ctacatcgag ctggcaagtt cgacgagctg gtggacactg
     2821 agggcaccat cggatttgat ctggtaccag ataccgctgt tgacccaggt agcgttgttt
     2881 ttgccgtaaa catagagggt gtggccggcg gtttggacgg tttggtaatc ttggccaatg
     2941 ccggccacga agtcagaacg gagggtttga ctgtcccagg ccgagagttt ttcggagatg
     3001 gcgtaggcgc ggctatcgct attgctttgg aactgcatgg cgacgttgcc gggactgtaa
     3061 ttgaggtgtg caaaactgta gccaatcggc tgataacttg gcaaattggc cgtgaaaccg
     3121 gcttttgatg aggccacttg caactggatg gaagtcaaat tggcgtacaa g
//
LOCUS       DATNGP010000057         3002 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_137024, whole genome shotgun sequence.
ACCESSION   DATNGP010000057 DATNGP010000000
VERSION     DATNGP010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3002
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137024"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1034)
                     /gene="pyk"
                     /locus_tag="VN778_01385"
     CDS             complement(<1..1034)
                     /gene="pyk"
                     /locus_tag="VN778_01385"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:TIGR01064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HXR49660.1"
                     /translation="MRPTGHISGVPITEYKRTKIIATIGPATAGYESIKRLIMAGANG
                     LRLNFSHGSHAEHAESIVRIRKASLELNKPVAIIQDLQGPKIRLGDFDGEINVKEGEA
                     LRLGYEADYTASGVIPIQYDLAAKVKAGERLYLYDGRVRARVTSITGSVIHVTALNSG
                     MLLKRKGINLPDTDLAGDVITEKDKADLAFGATQDIDYVAQSFVQTADDLKALRELMN
                     QHHMTAKLIVKFETRAAVDNIDAIVQEADAIMVARGDLAGETSAETVPIVQHKLIGLG
                     QRYAKPVIIATQTMLSMTDSPAPTRAEVSDVATAILLGADAVMLSEETAMGKYPTETV
                     ATMKRIMLRC"
     gene            complement(1210..1620)
                     /locus_tag="VN778_01390"
     CDS             complement(1210..1620)
                     /locus_tag="VN778_01390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49661.1"
                     /translation="MKIYLCASKSFFDKVNGIKAELEKLGHAVTPPNGFDEPAAESRH
                     QSMTAQEYADWKTGMIRHDGEVVAAHDAVLVLNFEKNGQQNYIGGAAFLEMFKAWDLG
                     KKVFLYNAIPDNMLKDEIIGLQPLVINRDLSLII"
     gene            complement(1661..2149)
                     /locus_tag="VN778_01395"
     CDS             complement(1661..2149)
                     /locus_tag="VN778_01395"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HXR49662.1"
                     /translation="MVIKSELLYEGRLFRLEYRDCDSFDELPRELITQVYGVCFLGDQ
                     LLIVHNGKKDTWGFAGGTTEPGETIEQTLVREVREESNMRVISWQPIGYQIVTSPNGV
                     VQYQLRVYCQIEKLGEFEGDPGGVVDKIELVSPNRVKRYFDWHEIGDRIVERALEIHA
                     SR"
     gene            2282..2752
                     /locus_tag="VN778_01400"
     CDS             2282..2752
                     /locus_tag="VN778_01400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49663.1"
                     /translation="MRGIAIGLEWVYGKVTGKGKEWAADKQRYKEALAREFERNVEAK
                     LALTPRDAQRIGEAALRSARESSRYGDFYLLSTLRAAAYFAFARLTEIQREQSSDAII
                     EARRIGARAIVGAERKGWEDFAISGYASAAAQLTHAGLPELPAQKDTIAPLESE"
     gene            complement(2778..>3002)
                     /locus_tag="VN778_01405"
     CDS             complement(2778..>3002)
                     /locus_tag="VN778_01405"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXR49664.1"
                     /translation="KFYRATNARTARPDGTGLGLFMAQKVIVAQGGSVIFKSKEGQGS
                     TFGFSFSKGQVAVKGSRGPNAEVAKAARLA"
BASE COUNT          721 a          816 c          735 g          730 t
ORIGIN      
        1 tcacaacgca gcataatccg cttcatagtc gccaccgttt ccgtcggata cttgcccatt
       61 gccgtctctt cgctcagcat cacggcgtct gcaccgagca gaatggcggt agccacatcc
      121 gagacttcgg cgcgggtagg cgctggcgag tcggtcatac tcagcatcgt ctgtgtggcg
      181 atgatgaccg gcttggcgta gcgttgtccc agcccgatca gtttgtgctg aacgatcggt
      241 accgtttccg ccgatgtttc gccggccaga tcgccgcgcg ccaccatgat cgcgtcggct
      301 tcttgaacaa tagcgtcaat attgtcaact gccgcacgag tctcaaattt aacgatcagc
      361 ttggcagtca tgtggtgctg attcataagt tctcgcagcg ctttcaggtc gtcagcggtc
      421 tgcacaaaac tctgagccac ataatcaata tcctgcgtcg caccaaacgc cagatcagcc
      481 ttgtcttttt cggttataac atcgcccgct aaatccgtat cgggcagatt gatgcctttg
      541 cgcttgagca acatacctga atttaacgcg gtgacatgaa tgacgctacc ggtaatgctt
      601 gtgacccttg cccgcactcg cccatcgtac aaatacaagc gttcaccggc tttgactttt
      661 gctgccaagt catactggat tggaataaca ccacttgcgg tgtaatcagc ctcgtagccc
      721 agccgaagcg cctcgccctc tttcacgttg atttcgccgt caaaatcgcc aaggcggatc
      781 tttggcccct gtaaatcctg aataatcgcc accggtttgt tgagctccaa gctagctttg
      841 cggatgcgca caatagactc cgcatgctcg gcatgcgaac cgtggctgaa gttcagccgt
      901 agaccattgg cgccagccat aatcagccgc tttattgact catagccggc tgtcgcaggc
      961 ccaatcgtgg caataatttt tgttcgttta tattcggtaa tcggaacacc agaaatgtgg
     1021 ccggtagggc gcatgagcaa gcctttcaat tttaagatat ggtatgtagg caaagaaaac
     1081 ctctcggaga tgccgagagg tagcttaccc taattcgttt ctggagcggt cagtcagtgc
     1141 tctttataac aaactaacta ttgttatagc ataagccgca aagataggca accgttttcc
     1201 acaacaatgt tagataatca acgaaagatc gcggttaatg accaggggtt gtaagccgat
     1261 tatttcatcc ttcagcatgt tgtccggaat ggcgttatac agaaacactt ttttgcccag
     1321 gtcccatgct ttgaacatct ccaggaatgc tgcgccacca atgtagttct gctggccgtt
     1381 tttctcgaag ttcaggacca acaccgcgtc gtgagccgca accacttcac catcgtgccg
     1441 gatcatgccc gttttccagt cggcatattc ctgagcggtc attgattggt gccgtgattc
     1501 agccgccggc tcatcgaacc cgtttggtgg agtgacagcg tgacccagtt tctcaagttc
     1561 ggccttaatc ccattgacct tgtcaaagaa gctcttgctc gcgcacaggt agattttcat
     1621 aaacacaata gtaacaagga attagcgtgg gctcagccaa ttagcggctg gcatgaattt
     1681 ctagagcacg ctccacgatt ctgtcgccga tctcgtgcca gtcgaaatac cgctttaccc
     1741 gattagggct taccagctcg atcttgtcca cgacaccgcc agggtcaccc tcaaactcgc
     1801 caagcttttc aatctggcag tagacacgaa gctgatactg tacgacacca tttgggctcg
     1861 ttactatctg atagccaatg ggctgccagc taataacgcg catattcgac tcttcccgaa
     1921 cttcgcgtac caaagtctgc tcaatcgtct cgcccggctc agtcgtacca ccagcaaaac
     1981 cccatgtatc tttcttgccg ttatgcacga tcaggagctg gtcccccaag aagcaaacgc
     2041 catatacttg cgtaatcagc tcgcgcggta gctcatcgaa actgtcgcaa tcgcgatatt
     2101 cgagtcggaa aagcctgcct tcgtataaca gctctgattt gattaccatg atactaaaag
     2161 tatataccga ttccttcgag cagcccgaca atacatactt aattcagcac gcatgatgtt
     2221 gtagcagttg tcatcaagac tacttagcag tataatatac ttgattagcg ggaggcttga
     2281 tatgcgaggt atagccattg gtttagagtg ggtctacggc aaggtaacgg gaaagggcaa
     2341 agaatgggcc gccgataagc agcgatataa agaggcattg gcgcgcgagt tcgagcggaa
     2401 cgtggaggcg aaactagcac ttacaccccg agacgcacaa cgaattggag aagccgcgct
     2461 gaggagcgcc agagagagta gcagatacgg cgacttctac ttactgagca ccctacgggc
     2521 cgcagcctac tttgcctttg cccgcctaac cgagatccag cgcgaacaat ctagtgatgc
     2581 aataatcgag gcgagacgca ttggagccag ggccatagta ggtgctgagc gcaaagggtg
     2641 ggaagacttt gcaatatcag gctatgcttc tgctgcggca caacttaccc atgcagggtt
     2701 accagaactg cctgctcaaa aagatacgat tgccccctta gagtcagagt aataatgctt
     2761 gattagcttc tggtatttta agcaagcctt gcggccttag ctacttccgc gttgggaccc
     2821 cgagaacctt tgactgctac ttgacccttc gagaagctga agccgaaggt gctgccttgg
     2881 ccctccttgc tcttgaaaat gaccgaaccg ccttgggcaa cgatcacttt ctgggccatg
     2941 aataagccga ggcccgtgcc atccggacgg gctgtacggg cgttcgtggc gcgatagaac
     3001 tt
//
LOCUS       DATNGP010000058         2005 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_336612, whole genome shotgun sequence.
ACCESSION   DATNGP010000058 DATNGP010000000
VERSION     DATNGP010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336612"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..312
                     /gene="rnpA"
                     /locus_tag="VN778_01410"
     CDS             <1..312
                     /gene="rnpA"
                     /locus_tag="VN778_01410"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HXR49665.1"
                     /translation="GYNSVRTVYQHGKSVRGPLLSLKFSQRETSRPYRVAVVVSRKVH
                     KSAVIRNRIRRRMYECVRRSESRIGGGTDLVFTVFNDQMAELPAAKLQTIIDDLLAKT
                     T"
     gene            374..1336
                     /locus_tag="VN778_01415"
     CDS             374..1336
                     /locus_tag="VN778_01415"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HXR49666.1"
                     /translation="MFTTLIVQPIFNLLVLVYALLPGHNFGLALIIFTIIIRLLLWPL
                     VKKQLHQAKAMQKLQPELKRIKKEAKGDRQKESQLLMELYKERGINPFSTFPILIIQL
                     IILIGLYSGLRRVISNPHELVSFAYPSLQHLGWMQTLAHNIHRFDNTLFGVVNLNRAA
                     LNPHGGVYWPAMLIVVGSAVAQFYQSKQLMPQSKDQRGLRQILRAAGEGKQADQAEVN
                     AAVGRSTRFLLPVMIFLFTVNLASALSLYWLTGGVVAYIQQAFILREDEEEMEELADG
                     GKLSKDVKNIPEAEVITSTPKKPSSKPKKAANAARKRAAKRRKR"
     gene            1333..1797
                     /locus_tag="VN778_01420"
     CDS             1333..1797
                     /locus_tag="VN778_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008578907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HXR49667.1"
                     /translation="MNDSMEEAILYAKKYLEDLLSFFGLNTDVYATSDDEVIQLNVPS
                     THMNGFLIGQHGENMRSLQYLTSTALKNNDYSHTRVNVDIAEYKKQRADRLRDKAETW
                     MKQVQGSGEPLDLQPMNAADRRIVHQLAADYGLSSDSVGDGRDRHIVLKPSE"
     gene            complement(1807..>2005)
                     /locus_tag="VN778_01425"
     CDS             complement(1807..>2005)
                     /locus_tag="VN778_01425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49668.1"
                     /translation="LKAALLMAVFILFLQGGGVLTYITDSNQAWYWQNRTIVSLNKVA
                     QQVVKPLIIIKTPLRSFGSVN"
BASE COUNT          500 a          562 c          519 g          424 t
ORIGIN      
        1 ggctataaca gtgtgcggac tgtgtatcag cacggtaaga gcgtgcgcgg tcccctgttg
       61 agcctgaagt tcagccagcg cgagactagc cggccatacc gggtagcggt tgtcgtcagc
      121 cgtaaggttc acaaatcggc ggtgatccgt aaccgcatcc gccgccgcat gtacgagtgc
      181 gttcgtcgct ccgaaagccg gatcggaggc ggcaccgacc tggttttcac cgtcttcaac
      241 gatcagatgg ctgagctacc agctgccaaa ctccaaacga tcatcgatga cctgctagct
      301 aaaaccacat gacagcttcc tgctacctcc tgtatagtgt tataagaaat aaccgggaag
      361 ttatctaaaa cacatgttca ccaccctcat tgttcagccc atttttaatt tattagtgct
      421 cgtttacgcg ctgttgccag gtcataattt cggcctggcg ctaatcattt tcaccataat
      481 tatccgcttg ctactatggc cgcttgtcaa aaagcagctg caccaagcca aagccatgca
      541 aaagctgcaa ccggagctga agcgcatcaa aaaagaagcc aagggcgatc gccagaaaga
      601 gtcgcagctg ctcatggagc tctacaagga gcgcggcatc aatcccttca gcaccttccc
      661 gatcctcatc atccagctca ttatcttgat cggcctgtat agcgggctgc gacgcgttat
      721 cagcaatccg cacgaactag ttagttttgc ctaccctagc ctgcagcatc tgggctggat
      781 gcagacgctg gctcacaata tccatcgttt tgacaacacg ctctttgggg tagtgaacct
      841 caaccgggca gcgctcaacc cgcacggcgg ggtgtactgg ccggcaatgc tgattgtcgt
      901 cggtagtgcg gtggcgcagt tctaccagag caagcagctc atgcctcaga gcaaagacca
      961 gcgcggtttg cggcagattc tgcgggctgc cggcgaaggt aaacaagccg accaagctga
     1021 agtcaatgcg gctgtcggac gcagcacccg cttcttgctg ccagtgatga ttttcctctt
     1081 cacggtcaac ctcgcttcgg cgctgagctt gtactggctg accggcggcg tggtggctta
     1141 tattcagcaa gcctttatcc tgcgcgaaga tgaagaagaa atggaagaac tggccgatgg
     1201 cggcaagctg tcgaaagatg tgaaaaatat tccggaagct gaagttatca caagcactcc
     1261 caaaaaaccc tcctccaaac ccaaaaaagc cgctaacgct gcccgaaaaa gggcagctaa
     1321 gaggaggaaa cgatgaacga ctcaatggaa gaagcgattc tgtacgccaa aaaatacctg
     1381 gaagacctct tgtcattttt tggtctcaac accgatgtct acgccaccag tgatgacgag
     1441 gtgatccagc ttaatgtacc aagtacacac atgaacggct ttctgatcgg tcagcacggt
     1501 gagaatatgc gctccttgca atatctgacc tccaccgcgc tcaaaaataa cgactatagt
     1561 cacacccggg tcaacgtcga catcgcagag tacaaaaagc agcgggccga ccgcctgcgc
     1621 gacaaagccg aaacttggat gaagcaggtc cagggctccg gcgaaccgtt ggatttgcag
     1681 ccgatgaacg ccgccgaccg ccgcattgtc catcagctgg cggccgatta tggcttgtcc
     1741 agtgactccg tcggcgacgg ccgggaccgc catatcgtac ttaagcccag cgagtaaacc
     1801 ctgaggctaa ttgacgctgc cgaaactgcg tagcggcgtc ttgatgatga taagcggttt
     1861 cacgacttgc tgcgcgacct tattgaggct gacaatcgtg cggttttgcc agtaccatgc
     1921 ctggttgcta tcagtgatgt acgtcagcac gccaccacct tgcaggaaca gaataaagac
     1981 ggccatgagc agcgctgctt tcaga
//
LOCUS       DATNGP010000059         6424 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_3074258, whole genome shotgun sequence.
ACCESSION   DATNGP010000059 DATNGP010000000
VERSION     DATNGP010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6424
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3074258"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            411..1007
                     /locus_tag="VN778_01430"
     CDS             411..1007
                     /locus_tag="VN778_01430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49669.1"
                     /translation="MSKINHSEALKATTVSRMRLLQISSSGAAKINWVDLLQPARKQL
                     ATSPEFHENLAIVTHFKSPRPGNPGIGESLYVVSNTESRGPSFANKSQILEKGRLTRH
                     MTNWLACTAMSHYWDPDLNDFLPLMQGKPFDRESLRSRVMQATFDYNLNILDEIRISG
                     LEKDPSTELTRQNAYFMLQTIHSMGYWPETGTSADFAE"
     gene            complement(1038..2975)
                     /gene="priA"
                     /locus_tag="VN778_01435"
     CDS             complement(1038..2975)
                     /gene="priA"
                     /locus_tag="VN778_01435"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HXR49670.1"
                     /translation="MYYYLVWVRSARYHGSDPLTYSSERHLPTGSIVQVELQKELVLG
                     FISGPISEPRFKIKPIARVFDLPPLPAHLIKLGTWLQQYYPAPLGFIAQQFLPANLSE
                     KQLAAELMPAGQQPKIKNLPPLTNEQTAALEAMQERDTYLLHGVTGSGKTRLYIELAS
                     QMIAKGRSAIILTPEISLTSQLAGNFRKVFGERVIVLHSQQAPSERQQAWLTCLRAKQ
                     PVIVIGPRSALFSPIEKPGLIVLDEAHESAYKQEQAPQYQAGRIASYLAGLTRSTLIL
                     GSATPSVSDYYLAQQKQKPIITLTQLAQPDVHPKTEIVLVDRKDHSLFSRSPYLSQEL
                     TKAVEAGLGKGEQSLLYLNRRGTARLVLCENCGWQATCPHCDIPLTYHGDHHQLRCHS
                     CNYHASAPTSCPNCGHAGIIFKTAGTKAIVEDVQRLFPNARVARFDTDNVKAERFEQH
                     YEAARSGQIDILVGTQLLAKGLDLPKLSTLGILLADTSLYLPDFSAQERTFQLVSQVL
                     GRIGRGHVAGRAIIQTYHPDHPVLQAAIAGDYQTFYASELAERQQFFFPPFCYLLKLT
                     ARRASLKAAESAAEKLKTGIEQAGYRVRVEGPAPSFYERFQNKYQWQLVIKAVDRSEL
                     LKIINTLPANWSYDIDPLDLL"
     gene            3074..3373
                     /locus_tag="VN778_01440"
     CDS             3074..3373
                     /locus_tag="VN778_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HXR49671.1"
                     /translation="MLDVFITSRVRRKIIVIYAKYPDFKTHVRGLAKLIKEDAGNIQR
                     ELKRLESVGFLISERQGNTKVYSTNKDFLIFRELQSIVLKSQQSQRSAPGRRVTG"
     gene            3420..3635
                     /gene="rpsU"
                     /locus_tag="VN778_01445"
     CDS             3420..3635
                     /gene="rpsU"
                     /locus_tag="VN778_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HXR49672.1"
                     /translation="MIQVTRKDTKESTENLLRRFNRKVQQSGSISVVKGNQFFAKPTS
                     KSERRRKAIIRQQRKALKLKKIKLGQR"
     gene            3642..4088
                     /locus_tag="VN778_01450"
     CDS             3642..4088
                     /locus_tag="VN778_01450"
                     /inference="COORDINATES: protein motif:HMM:NF020977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HXR49673.1"
                     /translation="MLEQKLEQDLKTALLAGDAQRVSTLRGLKSVLLNEKVAKGKRES
                     GLTDEEVLPLLSKQAKQRQESADLYVQGGDQVRADKELSEKAIIEEYLPAQLSEAEIA
                     KVVDEVIAETGAQGPAGMGQVIGQVKQRLGASADGAVIAKIAKEKL"
     gene            4097..4621
                     /locus_tag="VN778_01455"
     CDS             4097..4621
                     /locus_tag="VN778_01455"
                     /inference="COORDINATES: protein motif:HMM:NF012624.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HXR49674.1"
                     /translation="MGVAGAGKSMQGRLFADEHGYAWISTGELFRVLVTGKRRQQMLE
                     GKLLSDDEVIKLVDKTLKMIDVGQEFVLDGFPRTIVQADWLLKQIHEDRLNLTAVVNL
                     VASPEIVKERLLSRGRQDDTEEAIRKRFTEYESTTKPILDYFRKENVPVHDIDGARDP
                     IIVHDDIMSRLDKQ"
     gene            4629..5384
                     /gene="map"
                     /locus_tag="VN778_01460"
     CDS             4629..5384
                     /gene="map"
                     /locus_tag="VN778_01460"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HXR49675.1"
                     /translation="MQTRIKTDSEIKAMRESGRMLATVLEFLKPNVQAGVSTKQLADE
                     ARQELEKLGGEPAFLGYQGYQDVICISVNDEVVHGIPNAHRIVAEGDIVGLDFGVNYQ
                     GMITDSAVSVIAGKPKTQGHIRLLEDTANALQVGIHAVHDRVRTGDIGAAIENSLKHR
                     KYGIVRDLVGHGVGHHVHEDPNIPNYGRANSGPWLDKGMTIAIEPMVTLGTERVYIAP
                     DGWTVRTADGSWSAHFEHTVLITDDGAEILTTT"
     gene            5430..>6424
                     /gene="ftsA"
                     /locus_tag="VN778_01465"
     CDS             5430..>6424
                     /gene="ftsA"
                     /locus_tag="VN778_01465"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HXR49676.1"
                     /translation="MNGSNQEHFIGLDIGTSTVRCVVGMFDPNGSSMPSIIGHGQAPN
                     QGMRRGAVVHVDEVADAIVHAVTEAERISGKQIKHATVNVNGSHVSGLDSEGVIAISA
                     ANREITPEDRLRVEEAATIVSLPPNREIVQFFAKNYSLDGQRNIKDPVGMHGVRLEVD
                     AHMVTAASPNLRNLDMAMEKAEVQPAHHTVSGLAAAEAVMTRQQREAGTALIDIGAGT
                     TNLIVFEDGEVQHVAVLPIGGQHITNDLAIGLKTDIDIAEQVKIEHADLRENPKKINV
                     MVKVGDKTHNFPFEVVAMVVEARVDELLDYVNKELVKIKRAGKLPGGIVLTGGTSR"
BASE COUNT         1606 a         1613 c         1800 g         1405 t
ORIGIN      
        1 tgaacttaag gaggaagaag aggaaaatga gtctgaaata ccgctcgccc agcgcttcac
       61 ccaagcgaga atgacgaacc tcctgatgcc tttaggccag ctcgataagc gccacgtcgt
      121 cgactaacta ttttctctaa aacaggccta tgccagagtc ataccaaagt cgtatgaaaa
      181 catatgtttt ggcatgactc tcatacgact cttgcactac cggttttcgg tggacaatgt
      241 gcttcaaatg tcggaccttg ttcgccgata ccccacgggg aacattaggc gaagggttta
      301 aaaagtcatg aggccacacg gcttggttcg aactcttcat tctcaggaaa ctattgaatt
      361 atatgcagct ttgttatata gttaatttat agtaacggca cctggaacta atgagcaaaa
      421 ttaatcattc agaagcatta aaagctacga ccgtttcgcg catgcgcctt ctgcaaatat
      481 catcttcagg tgcggcaaaa atcaactggg tggatttgct gcagcctgca agaaaacaat
      541 tagctacatc gcctgaattt catgaaaatc tggccatagt gacacatttt aaaagtccaa
      601 ggcctggtaa tccaggaatt ggtgaatccc tatatgttgt atccaataca gaatcaagag
      661 ggccttcttt cgctaataaa tcgcaaatcc tggagaaggg taggctaaca agacatatga
      721 caaactggct agcttgtaca gctatgtctc attattggga cccagatcta aatgatttct
      781 taccattgat gcagggaaag ccttttgatc gtgaatctct tcgtagtcgt gtgatgcaag
      841 ccacttttga ttacaatctg aacatattag atgagataag aatttcgggt ttagaaaaag
      901 atccttctac agaattaaca cgtcaaaacg cgtactttat gcttcaaaca atccatagca
      961 tgggctactg gccagagact ggcacttctg ctgatttcgc ggaataatgc tataagccca
     1021 gcaagtaata tctccagtta aagcaaatcg agggggtcga tgtcgtagga ccagttggcg
     1081 ggcaaggtat tgatgatttt gaggagttcg ctgcggtcca cggctttaat gactagttgc
     1141 cattggtatt tgttctgaaa gcgttcgtag aaagaggggg cgggaccttc gacgcggacg
     1201 cggtagccgg cttgttcgat gccggttttt aatttctcgg cggcactttc ggcggctttg
     1261 aggctggcgc gccgggcggt aagcttaagc aaataacaaa acggcgggaa gaagaattgt
     1321 tggcgctcgg ccagctcgga ggcgtaaaaa gtctggtagt cgccagcgat ggcggcctgc
     1381 aacacgggat ggtctggatg gtaggtttgg atgatggcgc ggccagccac gtggccccgg
     1441 ccgatgcgac cgaggacctg actaaccagc tggaaggttc tttcttgggc agagaaatcg
     1501 ggtagataca ggcttgtgtc ggctagcagt atccccagcg tactaagttt cggcaggtcg
     1561 aggcctttgg cgagaagctg ggtaccgacg agaatgtcta tctggccgct gcgagcggct
     1621 tcgtaatgct gctcaaagcg ctcagccttc acgttgtcag tatcaaagcg agcaacccgg
     1681 gcgttgggaa atagccgctg cacgtcttca acgatggctt tggtgccggc tgtcttgaag
     1741 atgatgccgg cgtggccgca gttcgggcag ctcgtgggcg ccgaggcgtg gtaattacag
     1801 ctgtggcagc gcaattgatg atggtcaccg tgatacgtca gcggtatgtc gcagtgcgga
     1861 caagtggctt gccagccgca gttttcgcac agcaccaggc gggcagtgcc gcggcggttg
     1921 aggtagagca gactttgttc gcctttgccg aggccggctt cgaccgcttt ggtcagctcc
     1981 tggcttagat agggcgagcg actgaagagg ctgtgatctt tacgatccac cagcacaatt
     2041 tcggttttgg gatgtacgtc cggctgggcc aactgggtga gcgtgatgat gggtttttgc
     2101 ttctgctgcg ctaaataata atcactcaca gacggtgtag cagagcctaa aattagcgtc
     2161 gagcgggtca ggccggccaa gtacgaggcg atgcggccgg cttgatactg cggcgcttgc
     2221 tcttgtttgt aagcgctttc gtgcgcttca tcgagaacaa ttaggccagg cttctcgata
     2281 gggctaaaca gcgcggagcg cggcccgata acgataaccg gctgcttggc ccgcaagcaa
     2341 gtcaaccagg cttgctgccg ttcactgggg gcctgctggg agtgcagcac aattacccgt
     2401 tcgccaaaca ctttccggaa attgccggcc agctgcgaag tcaggctgat ctcaggtgtc
     2461 aggatgatgg cggagcggcc tttggcaatc atctgcgagg ccagctcgat gtagagccgg
     2521 gttttgccgc tgccggtgac accgtgtaat aggtacgtgt cgcgttcctg catggcttcg
     2581 agggctgctg tttgctcgtt tgtaagggga ggtagatttt tgatttttgg ttgttggcca
     2641 gcaggcatta attcggccgc aagttgtttt tcggagaggt tagcgggcaa aaactgttgg
     2701 gcaataaagc cgagcggagc tggatagtat tgctgtagcc acgtgccaag tttgataaga
     2761 tgggccggca atggcggcaa gtcgaaaacg cgcgcgatcg gcttgatctt gaagcgcggc
     2821 tcactgatcg gcccactaat gaagccgagc accagctcct tttgcaattc aacttgcacg
     2881 atggagccgg tcggcaaatg ccgctcgctg gaataggtta gagggtcact gccgtggtag
     2941 cgagcggagc gaacccacac gaggtagtag tacatagtta taagtataag ctaagaaagt
     3001 gatttggctt gccaaagcaa aagttgcccg ttatactaat taatatcgtt gtttggataa
     3061 caataaggga catatgttag acgtattcat tacttcgcgg gtgcgacgca agatcattgt
     3121 catctatgcc aagtacccag actttaagac ccacgtccgc gggctggcta agctgataaa
     3181 agaagatgcc ggcaatattc agcgcgaact gaagcgcttg gagagcgtcg gatttttaat
     3241 cagcgaacgg cagggaaata ccaaagtcta ctccacgaac aaggactttt tgatctttcg
     3301 cgagctgcaa agcattgtgt tgaaatcaca acaatcccaa cgctcagcgc cgggccgccg
     3361 cgtgaccggc taacattagt cttgcaaatt tcacctctgt cagatacaat atcaccctga
     3421 tgatacaagt tacccgcaaa gataccaagg aatccaccga gaacttgctt cggcgcttta
     3481 accgcaaggt tcagcagtcg ggcagtatca gtgtggttaa aggcaatcag ttttttgcta
     3541 agccgacgag caagagcgaa cgccgccgca aggccatcat ccgccagcag cgcaaagccc
     3601 tcaagctcaa gaaaattaag ctgggtcaac gctagcacgc gatgttagag cagaaacttg
     3661 agcaggacct caagactgcc ttgctagctg gggatgctca gcgtgttagc acgctgcgtg
     3721 gcttaaaaag cgtgctttta aatgaaaaag tcgccaaagg taagcgcgaa agcggcctga
     3781 ccgacgaaga agtattgcca ttactcagca agcaagccaa gcagcggcaa gaaagcgccg
     3841 acctgtatgt ccaaggcggc gatcaggtgc gcgccgacaa ggaactgtcg gagaaggcca
     3901 ttatcgaaga gtatttaccg gcgcagctga gcgaagctga gattgccaag gtagttgatg
     3961 aagtgattgc cgaaaccggt gcccaagggc cggccggcat gggacaagtc atcggtcaag
     4021 taaagcagcg tttgggtgcc agcgccgacg gcgccgttat cgctaaaatt gcgaaagaga
     4081 aattatgatt atacttatgg gagtcgccgg ggcgggcaaa agtatgcaag gccgactgtt
     4141 tgccgacgag cacggctacg cctggatttc aaccggtgag ttgttccggg tactcgtgac
     4201 aggcaagcgc cgacagcaaa tgctggaagg caagctgctc agcgacgacg aagtgatcaa
     4261 acttgttgat aaaacactca aaatgattga tgtcggccag gagtttgtgc tggacggttt
     4321 tccccggacc atcgtccagg ccgattggct gctcaaacaa atccacgaag accgcctgaa
     4381 cctcactgcg gtcgtgaacc tcgtggcttc gcctgagatc gtcaaagaac gcctgctcag
     4441 ccgtggccgc caggatgaca ctgaagaagc tatccgtaag cgtttcacag agtatgaatc
     4501 aaccaccaag ccgattctcg attattttcg caaggagaac gtgccggtcc atgacattga
     4561 tggcgctcgc gatccgatta ttgtccacga cgacattatg agccggcttg ataagcaata
     4621 ggcagccgat gcaaacccgc attaaaaccg attccgaaat aaaagcgatg cgcgaaagcg
     4681 gccggatgct ggcaaccgtc ctcgaatttc tcaagcccaa tgtccaagcc ggggtttcga
     4741 ccaagcagct ggctgatgaa gcccgccaag aactcgaaaa actcggtggc gaaccggctt
     4801 tcctcggcta ccaaggctac caggatgtga tctgtatttc ggtcaatgac gaggtggtgc
     4861 atggtatccc gaatgcccac cggattgtcg ccgaaggcga catagtcggg ctggacttcg
     4921 gcgtcaatta tcaggggatg atcaccgatt cggccgttag cgttatcgcc ggcaaaccga
     4981 agactcaagg gcatattcgc ttgctggaag acaccgccaa tgcactgcag gtcggcattc
     5041 acgccgtcca cgatcgagtc cgcaccggcg atatcggcgc ggctatcgaa aattcgctga
     5101 aacaccgcaa atacggcatc gtccgcgact tggtcggcca cggtgtcggc caccacgtcc
     5161 acgaggaccc gaacatcccc aattacggcc gagcgaacag cggcccgtgg ttggacaaag
     5221 gcatgaccat cgccatcgag cccatggtca ccctcggcac cgaacgtgtc tacatcgccc
     5281 ctgacggctg gaccgtcaga acggccgacg gctcgtggtc cgctcatttc gagcacaccg
     5341 ttcttatcac cgacgacggc gccgaaatac tcacgactac ataagggctg cttgctgcag
     5401 attggcacta gcgttatact ggggcccaga tgaatggttc taatcaagaa cactttatag
     5461 gtctcgatat cggcacgagt accgttcgct gcgtggtcgg tatgtttgat ccgaatggta
     5521 gcagcatgcc gtcgattatc ggacatggcc aggcgccgaa tcaaggcatg cggcgtggtg
     5581 ccgtggtgca cgtcgatgaa gtagccgatg ccattgtaca cgccgtaaca gaggcggaac
     5641 ggatttcagg gaagcagatc aagcacgcca ccgttaacgt caacgggtcg cacgtcagcg
     5701 gtctggactc cgaaggcgtg atcgctatca gcgccgcgaa ccgcgaaatt acccccgaag
     5761 accgtttacg agtggaagaa gcggcaacta ttgtgtcatt gccgccaaac cgggaaatcg
     5821 tccaattttt tgccaaaaat tacagtctcg atggtcagcg caacatcaaa gatcccgtcg
     5881 gcatgcacgg tgtccgcctg gaagtcgatg cccacatggt tacagcggcc tcgccgaact
     5941 tgcgcaacct cgatatggcc atggaaaaag ccgaagtcca accggctcac cacaccgtat
     6001 ctggcctggc ggcagctgaa gctgtcatga cccgccaaca gcgcgaagct ggcacagcac
     6061 tcattgacat cggcgccggc acgacgaact tgatcgtctt cgaagacggg gaagtgcagc
     6121 atgtggccgt tttgcctatc ggtggccaac acattaccaa cgatctggct atcgggctca
     6181 aaaccgacat cgacatcgct gagcaggtca aaatcgagca tgccgatttg cgcgaaaatc
     6241 ctaagaaaat aaatgtcatg gtcaaagttg gcgacaagac gcataatttc ccctttgaag
     6301 ttgtggccat ggtggtggaa gcccgcgttg acgagctgct ggactatgtc aataaagaac
     6361 tcgtgaaaat taaacgcgcc ggcaagctgc cgggcggcat tgtgctgacg ggcggcacct
     6421 cccg
//
LOCUS       DATNGP010000060         5908 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_974743, whole genome shotgun sequence.
ACCESSION   DATNGP010000060 DATNGP010000000
VERSION     DATNGP010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5908
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_974743"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..769
                     /locus_tag="VN778_01470"
     CDS             <1..769
                     /locus_tag="VN778_01470"
                     /inference="COORDINATES: protein motif:HMM:NF017102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HXR49677.1"
                     /translation="DLTIASTDDNVISKPQVVTTALKSRADIQSYTSATGDTISSVAA
                     KFGVTSDSIRWSNNLTGDNLAAGTKLVIPPVNGLVYQVKTGDTADSLAAKYHASKDQI
                     IAYNDGELSGLQIGEQIIIPNATVATIPSYSSSGGGGFLWGSGPMYGYNGYDFGYCTW
                     YVATQISVPGNWGNASSWAYYARLSGWNVSKTPTVGSVAQTALAAGGEGHVAVVDGVN
                     GSMVHIRDMNNYGDGGGWDRVGSGWVPVGEFQNYIAR"
     gene            817..2091
                     /gene="obgE"
                     /locus_tag="VN778_01475"
     CDS             817..2091
                     /gene="obgE"
                     /locus_tag="VN778_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HXR49678.1"
                     /translation="MSFVDKVNVTAVAGKGGDGKLSFRHEKFIAKGGPDGGDGGDGGD
                     VVLLASRNQNTLAAFRYQKELRAEPGQPGGTSRKHGRSGPDLIVAVPVGTVASKADGS
                     VVADLVEDGQRAVVAKGGKGGFGNAHFVSSRRQAPQFAEKGEPGEQFDLQLELKMIAD
                     VGLVGLPNAGKSTLLSKLSNARPEIADYPFTTLTPNLGVVDIDKDNAVLFADIPGLIE
                     GAAAGKGLGHDFLRHIERTAVILHLIDAYNDDVAEAYQTVRAELEAYQPELIKRPEII
                     ALNKVEGLDNEIVDELMKQLHQVKPLETPIFAISAQSGEGLQQLLFIVKETVQAVRAM
                     PPEETEEPSLPVLTLTDTAEEWHVSREGTKFVVTGPKIEQFARRTDFSNDQGVRRLRD
                     IMKRMGILHELARQGIEAGQVIRIANHGEFTY"
     gene            2115..3569
                     /gene="sbcB"
                     /locus_tag="VN778_01480"
     CDS             2115..3569
                     /gene="sbcB"
                     /locus_tag="VN778_01480"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF008746.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008310 - single-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HXR49679.1"
                     /translation="MADSFFFYDLETSGFNPRTARIMQFAGQRTDMELQPIGDPVDIL
                     IKLTPDVLPQPDAVLITGITPQKTLADGISEAEFLKFFYKEVAKPDTIFAGFNNVRFD
                     DEFMRFLHYRNFYDAYEWQWRYNSSRWDLLDVVRMTRALRPEGIIWPFASDGKPTNRL
                     EFLTTVNKLDHLKAHDALSDVHATIAVARLIREKQKDMFDYLLSMRNKKKVLELVSNA
                     TPFVYTSGRYSSDFMHTTVAVLLAKHIEQDAALVYDLRYDPTPFLDMDVKQLIEAWRF
                     TKDPEAVRLPVKTLKYNRCPAVAPLGVIKDQATQDRLQLDLKTVSKHFNILKKHHESF
                     ATRVAEAVSELDKERAKTQQTALIDNEQNVDERLYEGFMPDNDKAVMSKVRAAKPDEL
                     PKFVSEFHDQRLQSLLPLYKARNHPKQLTQEERAAWDAFVAQKLLSGDQESRLAQYFA
                     RLQELASGKLTEQQQYLLEELRLYGESLVPTDAG"
     gene            complement(3643..5193)
                     /locus_tag="VN778_01485"
     CDS             complement(3643..5193)
                     /locus_tag="VN778_01485"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXR49680.1"
                     /translation="MSDTIAGRALELPHVQVNNPDLIAECLDDGKIAVVHRHATMEKP
                     YEVEVTATRHIANDVGHLSLHASFAFNETVIPSQIATLRERYLPEIKGLFLGSAGIGA
                     TYVGNEKKSLRLAFDDGKTSITTWFSRGGVPPYHFYASNNRRDGLNNNRISSSPPKFE
                     RATLEEDLANYMRLLSVVDRVLGGSSSKYTIDFIEQRSPRQQQRTSSKRSKNQHASKP
                     EGNQSEQAQANTKLRELATSLNEKYAPRLPNPNARLEDIHGIDPNDWEKLEERILVFS
                     NPELAKQWNVKSGGGILLLGPAGTGKTMTAAAICNKIGARIWQVDGPDIVNMYVGNSA
                     KYTAALFENAVKVSKLGPLVLLFDEIDSVVVSKDMHAERASVLGQFKKDLAYMANQVP
                     NVLVIGTTNHAENLDEAFIRSGRFDVKIYMKHPDERTRLQIFMDHITLDEAQGTRQFD
                     ETAKFDFAALAKSTDGMTGADIALIVKNCRYKKFRAHVQGSIHPGSISQHDIINQINI
                     FREQPQAF"
     gene            5354..>5908
                     /locus_tag="VN778_01490"
     CDS             5354..>5908
                     /locus_tag="VN778_01490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49681.1"
                     /translation="MNPRHERRVGRRTQAAGPRPPELLPRFNRHAREQQGQMYMPEPV
                     TSKESTRAFGIRRGVEAMAARVIWDELRRMKIAKELPEAIQLSTQPALHLELLPVEVL
                     DRRARRLQRESSDEQKIKSALETKCRRTKDTPRTADIVDLRFTRGTHLSLIVSCPKIT
                     QEGVGIGEVLRRHDIRPRQRTWDAQ"
BASE COUNT         1478 a         1431 c         1560 g         1439 t
ORIGIN      
        1 cgatctgacg atagcttcca cggacgacaa cgtcatcagt aaaccccagg tcgttaccac
       61 cgccctgaaa tcgcgggccg atatacagag ctacacttcg gcaactggcg atactatttc
      121 gagcgtcgcc gccaagtttg gcgttacttc cgatagcatc cgctggtcga ataatctgac
      181 aggcgacaat ctggcagctg gcaccaagtt ggttatcccc ccggttaacg gtcttgtcta
      241 tcaggtcaaa accggcgaca ccgccgatag tctagccgct aaatatcacg cgagcaaaga
      301 tcagatcatt gcttacaatg acggcgagtt gtccggtttg caaatcggcg aacagattat
      361 tatccctaac gcgacggtgg ctacgatacc aagttacagc agtagcggcg gtggcggctt
      421 tctctggggc agcggcccaa tgtacggtta taatggttac gactttggct actgtacctg
      481 gtacgtggcg acgcaaatca gcgtgccggg gaactggggt aacgccagca gttgggccta
      541 ctacgcccgc ttgagcggct ggaacgtcag caaaacaccc acggtcggct cggtcgccca
      601 aaccgcactc gcagctggcg gcgaaggcca cgtggccgtt gtcgatggcg ttaatggcag
      661 catggtgcac atcagggaca tgaataatta cggtgatggt gggggctggg accgcgtcgg
      721 cagcggctgg gtaccggtcg gagaattcca aaattacatt gctcgctaag tgctactcta
      781 ccaactgcca gccaaaaact atacactaag agcagcatga gttttgttga taaggtaaat
      841 gttacggcgg tcgccggcaa aggcggtgac ggcaagctga gtttccggca cgaaaagttc
      901 atcgccaagg gaggtccgga cggcggtgat ggtggtgacg gtggtgatgt cgtgctgctc
      961 gccagccgca atcagaacac tctagcagcc tttcgctatc agaaagagct tcgggctgag
     1021 cccggccagc cggggggcac aagccgaaag cacggccgga gtggcccgga tttgatcgtc
     1081 gcagtacccg tcggaacagt ggcctctaag gctgatgggt cagtagtggc cgacctcgtt
     1141 gaagatggcc agcgcgcagt cgttgccaag ggcggtaagg gtggttttgg caatgcccac
     1201 ttcgtgtctt ccaggcggca agcaccgcag tttgccgaaa aaggtgagcc gggggagcag
     1261 tttgatttgc aacttgagct taaaatgatt gctgacgtgg gtttggtggg gttgccgaac
     1321 gccggtaaat cgactttact tagcaagtta agtaatgcgc gcccggagat agcggactat
     1381 ccttttacta ctttgactcc caatttaggg gtcgtcgaca ttgataaaga caatgcggtg
     1441 ctttttgctg acattccggg cctgatcgaa ggggctgctg ccggcaaagg tctaggccat
     1501 gactttctca ggcacattga acgtacggcc gtaatcttgc atctgattga tgcctataat
     1561 gatgacgtag ccgaggctta tcaaacagtc agagccgagc tagaggctta ccagcctgag
     1621 ttgatcaagc ggccggagat tattgccctg aacaaagtcg agggcttgga taatgaaatc
     1681 gttgatgaac tgatgaagca gctacaccaa gttaagccgc ttgaaacacc aattttcgct
     1741 atttcagccc agtccggcga aggtctgcag cagcttcttt ttatcgtcaa agagacggtt
     1801 caagcggtac gggcaatgcc tccggaagaa acggaggagc cttcgttgcc agttctaacg
     1861 ctaacagata ccgcggagga gtggcacgtg agccgggaag gcaccaagtt cgttgtcacc
     1921 ggccccaaaa tcgagcaatt cgcccgccgt accgactttt ccaacgacca aggcgtaaga
     1981 cgcctccgcg acattatgaa acgtatgggc attctccatg agctcgctcg ccagggcatt
     2041 gaagctggcc aggttatccg gatcgccaat cacggggaat tcacctacta aaggtgttac
     2101 actggtagct actgatggcg gacagctttt tcttttacga tctagagaca tccggattta
     2161 acccacggac ggcgcggatt atgcagtttg cggggcagcg gacggatatg gagctgcagc
     2221 cgatcggcga tccggtcgac atcctcatta agctaacgcc cgatgtgttg ccgcagccgg
     2281 atgcggtgct gattaccggc atcacgccgc aaaaaacgct cgcagacggc attagcgaag
     2341 cggaattttt gaaatttttc tataaggaag tggcgaagcc cgacacgatt ttcgctgggt
     2401 tcaataacgt gcgttttgac gatgaattta tgcggttttt gcactaccgt aatttttatg
     2461 acgcttatga gtggcagtgg cgatacaata gctcccgctg ggacttgctc gatgtcgtgc
     2521 gcatgacacg ggctctgagg ccagagggta tcatctggcc gtttgcgtca gatggcaaac
     2581 cgaccaatcg gctcgagttc ctgactaccg tcaacaagct cgaccacctc aaagctcatg
     2641 acgcgctcag cgacgtgcat gctactatcg ccgtggcgcg gttgatccgc gaaaagcaaa
     2701 aagacatgtt cgattatctg ctgagcatgc gtaataaaaa gaaggtgctc gagctggtta
     2761 gcaatgctac accttttgtc tacacttcgg gccgctacag cagcgatttc atgcatacga
     2821 cagtggctgt attgctggct aaacacatcg aacaggatgc ggcgctggtg tatgacttgc
     2881 gctacgaccc cacccctttt ctcgacatgg atgtcaaaca attaattgag gcctggcgat
     2941 ttaccaaaga ccccgaagcc gtacgcttgc cagttaagac gctgaagtat aatcgttgtc
     3001 cagctgtagc gccacttggg gttatcaaag atcaagcgac ccaagatcgt ctgcaactcg
     3061 acctcaaaac tgtttctaag cacttcaata ttttgaaaaa acaccacgag tcctttgcta
     3121 ctagggttgc ggaggctgtt tcagagctcg acaaagaacg ggccaagact cagcaaacgg
     3181 ccctgatcga caacgaacag aacgttgacg aacggctgta cgagggcttc atgccggaca
     3241 atgacaaggc ggtgatgagc aaggtgcgcg ctgccaaacc tgacgaactc cctaagtttg
     3301 tcagcgaatt tcatgatcag cgtctgcaaa gcctactccc gctctacaaa gcccgcaacc
     3361 atcccaagca gctgacccaa gaagagcgag cggcgtggga tgctttcgtt gcccagaagc
     3421 tcctgagtgg cgaccaagaa agccgcttag cccaatattt tgcccgtctg caagagctag
     3481 ctagtggcaa gcttaccgag caacagcaat acctgcttga agagttacgg ctctacgggg
     3541 aatctctggt acccaccgac gccggttaaa agtgaccgtg tttggggtga cgaaaggccg
     3601 aacacatagc tgaaagcgag ataacgatac tcgttactga atttaaaaag cttgtggttg
     3661 ttcgcgaaaa atattgattt gatttatgat gtcgtgttgc gagatggacc caggatgtat
     3721 gctcccctgg acgtgcgccc gaaatttttt gtaacggcaa ttcttgacga taagagcaat
     3781 gtcagcacct gtcatgccat cggtagattt ggcgagggca gcaaagtcaa attttgcagt
     3841 ttcatcaaat tgtcgtgtgc cctgagcttc atcaagagta atatgatcca tgaaaatttg
     3901 gagtcgagtg cgttcatcag gatgcttcat atatattttg acatcaaatc tgccagaacg
     3961 aataaaggct tcatcaagat tttcggcatg gttagtagtg ccaatcacta aaacattagg
     4021 tacttgattt gccatgtatg ctaggtcttt tttgaattgt cccagtacag acgctctttc
     4081 agcatgcata tctttactga ctaccacgct gtctatttcg tcaaatagaa gtactagcgg
     4141 gccgagcttt gataccttca ctgcattttc aaataaagcc gcggtatatt tggcagagtt
     4201 accaacgtac atgttgacaa tatctggccc atcgacttgc caaattcgtg cgccaatctt
     4261 gttacagatg gctgccgctg tcatagtttt gcctgtaccg gcgggcccta gtagcaaaat
     4321 tccgccgcca gatttcacgt tccactgctt ggctagttcg ggattagaga agactaaaat
     4381 acgttcctca agtttttccc agtcattcgg atcgatacca tgaatatctt caagtcgggc
     4441 attggggtta ggtaaacgtg gagcatattt ttcattcaag ctggtggcta attcacgaag
     4501 tttagtatta gcttgtgctt gttcgctttg attcccttct ggtttgcttg cgtgttgatt
     4561 tttgctgcgt tttgatgaag ttctctgctg ttggcgggga cttctttgtt caataaaatc
     4621 aattgtatat ttcgacgatg atccgccgag gaccctgtcc actacagaga gaagtctcat
     4681 ataattggcg aggtcttctt ctaaggtagc acgctcgaac tttggcgggg atgaagagat
     4741 tcggttattg ttgagcccat cccttcggtt attggaggca tagaaatgat aaggtggcac
     4801 tccccctcga gaaaaccaag ttgttatact agttttcccg tcatcaaaag ctaggcgtag
     4861 ggatttcttt tcatttccaa cataggttgc ccctatgccg gcgctgccca gaaacagccc
     4921 cttaatctcc ggcaagtatc tttctcgtaa ggtggcaatt tgagagggga taacggtttc
     4981 attaaaagcg aacgaagcat gtaggcttaa gtgtccgaca tcattagcga tgtgtcttgt
     5041 cgcggtaact tcaacttcat acggcttctc catcgtagca tgacgatgaa ccaccgcaat
     5101 ctttccgtcg tcaaggcact cggcaatcaa atcaggatta tttacctgca catgcggtaa
     5161 ctctagtgct ctcccggcga ttgtatcact cataaaatta attttaaagt cgtttcagtt
     5221 gaagtatacg atatatatgt ttttgcggca aaaaacattt gttgtgtttt tcatgcatat
     5281 actcaagcta tatattattt taaaagcgag tagagttgtt gactggcaat cagtggtatg
     5341 atatagctaa tttatgaatc ccagacacga gcgtagagtt ggccggcgaa cccaagcggc
     5401 aggaccacgt ccacccgaat tattaccgcg ctttaatcgg cacgcccgag agcagcaagg
     5461 gcaaatgtat atgccagagc ccgtaacaag caaggaaagc acgcgagcct ttggtatacg
     5521 gcgcggcgtt gaggccatgg cggcccgggt aatttgggat gagctaagac gcatgaagat
     5581 tgccaaagaa ttgcccgaag ccatacagct tagcacgcaa ccagcacttc atttagagct
     5641 tttgccagtt gaggtactag accgtcgggc tcgacgtttg cagcgcgaaa gctcggatga
     5701 acagaagatt aaaagtgcac tggaaacaaa gtgccgacgc actaaagata cgcctcgaac
     5761 cgcggatatt gtggacttaa ggtttacgcg cggcactcac ttgagcctta tcgttagttg
     5821 tcctaaaatt acgcaggaag gggttgggat tggtgaggtg cttaggcggc acgacattcg
     5881 tcctaggcaa aggacatggg atgcgcag
//
LOCUS       DATNGP010000061         2818 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_2417031, whole genome shotgun sequence.
ACCESSION   DATNGP010000061 DATNGP010000000
VERSION     DATNGP010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2818
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2417031"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..359)
                     /locus_tag="VN778_01495"
     CDS             complement(<1..359)
                     /locus_tag="VN778_01495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49682.1"
                     /translation="MNKNNNMSQSKDKKPHMPQPATTLMRHAVVKPKTALKHRVKAVG
                     HLNAAASATLQPKPSVHALDAKRLRHAKQVAKSPAISRFGKILPARPSLPVFQPVVIE
                     SKPAAKQPLAAPTTTAEL"
     gene            453..1934
                     /locus_tag="VN778_01500"
     CDS             453..1934
                     /locus_tag="VN778_01500"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HXR49683.1"
                     /translation="MDYLDPQKELRHRIILLVGYVLIAIAITIGTLILVYEAYGFGLG
                     KNGNVIQNGLLFFSSQPHPANIYVNGILAKSQTNTRLVLPENIYHVTLTRSGYRPWQR
                     TIELLGGTVEHFDYPFLIPAKLTTKKEQPYNGAPGLMTQSPDRRWLLVQTPGSMTSFE
                     MYDLTTSPLKTPATFSLPSGILTKATTNEKWQLDEWADDNRHVLLEHDYDGKSEFILV
                     DRTNPDQSLNLNTTLATNPPELKLVNKKYDQYYLYTPTTGDLQTATLASTKVAPALQH
                     VLAFQSYGSDTILYVTNNGASPGKVLIKLQSGGQTYTLHSLPAGTNYLVDLTKYSGTL
                     YVAVGAGSENKVYIYQDPIGQLAAQPKQALVPAQVLRVNNPNYVSFSTNAQFIVAESG
                     THFGVYDIENQKGYQFTTNLPLDTPQIHASWMDGDRLNYVSGGKLVMLDYDNNNRQTL
                     TTASSNYVPAFPPNYHFVYAVAPSAATGQFELDQTALLTLTDQ"
     gene            complement(1937..>2818)
                     /locus_tag="VN778_01505"
     CDS             complement(1937..>2818)
                     /locus_tag="VN778_01505"
                     /inference="COORDINATES: protein motif:HMM:TIGR01076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class E sortase"
                     /protein_id="HXR49684.1"
                     /translation="KLRDVRTKTEVQAVIRDKVSAGGKLQAKHHLQSLLFGLSMGLIA
                     VVIFLFGFFNEVIIAPFIQPSRATAATPLIISTDSVAPTATPEVIIPKINVEIPVNYT
                     ETSTDENTIENDLQDGVVHYPTTVMPGQQGNTAFFGHSSNNIFNKGKYKFAFVLLHTL
                     VNGDTFYLTYNGKVYVYKVISRTIVDPSDVGVLGPVPGQTATATLITCDPPGTSLHRL
                     IVVGQQISPDPASDTAGTATASSSSSGTTTLPGNGPTAWTHFIRSAGGKISTVAILLV
                     VFIFVVRWVNAPRKSLA"
BASE COUNT          684 a          695 c          739 g          700 t
ORIGIN      
        1 agctcggccg tagttgttgg cgcagctagt ggttgcttgg ctgctggctt ggactcaatg
       61 accactggct ggaacacggg caatgatggt ctggccggta aaatcttacc gaaacgactg
      121 attgccgggc tcttggctac ctgtttggca tgccgcaggc gcttagcgtc gagcgcgtga
      181 actgagggct tgggctgcaa ggttgcgctg gccgcggcat ttagatgtcc gacagcttta
      241 acgcggtgtt tcaaagcagt tttcggtttc acgacggcat ggcgcatcaa agtagtggcc
      301 ggctgtggca tgtgcggttt tttgtctttt gattggctca tgttgttgtt tttgttcaca
      361 tttgtcactt ttaagctgtg agaatcatgt ataaacatct taaaccctct tgtagttccc
      421 cgcaagtccc cgcataatta tgtatcgaac caatggatta tctcgacccc caaaaagagc
      481 ttcgtcaccg cataattttg ctggtcggct acgttttaat cgccatcgcc atcacgatcg
      541 gcaccctgat cctagtttat gaagcttatg gttttggctt gggtaaaaat ggcaatgtca
      601 ttcaaaatgg ccttctcttc ttttctagcc agccgcaccc ggcaaatatt tacgtgaatg
      661 gcatcctggc gaaatcacaa accaatacca gattggtcct acccgaaaat atttatcatg
      721 tgacgctaac ccgcagtggc taccgtccct ggcaacggac aatcgagtta cttggaggta
      781 cggtcgagca ttttgactac cctttcctga tcccggctaa gcttacgaca aaaaaagaac
      841 aaccttataa cggtgcaccc ggcttaatga cccagagtcc cgaccgccgc tggctactgg
      901 tgcaaacgcc gggctcgatg acaagtttcg aaatgtacga tttgactacc agcccgctaa
      961 aaacaccggc taccttttca ctgccgtcag gtattttgac gaaggccaca accaacgaaa
     1021 agtggcaact tgatgaatgg gccgatgata atcgtcacgt cttgcttgaa catgactacg
     1081 acggcaagag cgagtttata ttagtcgatc gcacaaatcc tgatcagtcc ctgaatctta
     1141 atactacgct ggcgacaaac ccgcccgagc tgaagctcgt gaataaaaag tacgatcaat
     1201 attatctgta cacacccact accggtgact tacaaacggc aaccctggcc tcaaccaaag
     1261 tggcccctgc cctacagcat gtcttggctt tccagtcgta tggctcagat acgatacttt
     1321 atgtcacgaa taatggagcg tcccctggta aagtgctcat caaactgcaa agcggcggtc
     1381 agacgtatac tttgcatagc ttgcccgctg ggacgaatta tttggtggat ctgactaaat
     1441 atagcggcac gctgtacgtc gcggtcggcg ccgggagtga aaataaggtt tacatttacc
     1501 aagatccgat cggtcagctg gcagcccagc ccaagcaggc gctggtgcca gctcaggtac
     1561 tgcgcgttaa caacccgaat tacgtgagtt tttcgacgaa cgcccaattt attgtggcgg
     1621 aaagcgggac acacttcggg gtatacgaca ttgaaaacca aaaggggtat cagtttacta
     1681 ccaacttgcc gcttgatacc ccccagatcc atgccagctg gatggatggc gaccgcttga
     1741 attatgtaag tggcggaaag ctagtgatgc tggattatga caataacaat cggcaaacac
     1801 tgacgacagc aagtagtaac tatgtgccgg ctttcccgcc gaattatcat ttcgtttatg
     1861 cggtggcgcc aagtgctgcg actggacagt ttgagcttga tcaaacagcc ctgcttacgc
     1921 tgactgatca gtaagactag gccaagcttt tccggggcgc attaacccag cgcaccacga
     1981 agataaaaac aacgagcaat atagcaactg tgctaatctt accgccggcg gaccgaataa
     2041 aatgagtcca agcagtcgga ccgttgccgg ggagggttgt agtaccggag ctggagctgg
     2101 aggcagtagc tgtgcctgcc gtatcgctgg ccgggtccgg actgatctgt tgaccaacca
     2161 cgatcaggcg gtgcaagctg gtaccgggtg gatcgcaagt gatgagcgtg gcggtggccg
     2221 tctggccggg taccggaccg agcacgccga cgtcgctggg gtcaacaata gttcggctaa
     2281 ttaccttgta gacgtatact ttaccgttat atgtcagata aaatgtgtcg ccattcacga
     2341 gggtgtgcag taaaacgaag gcgaacttgt acttgccttt attgaagata ttattgctac
     2401 tatgcccgaa aaaggccgta ttgccctgct gccccggcat gacggtggtc ggatagtgta
     2461 cgacaccgtc ctgcaagtca ttttcaatgg tgttttcgtc ggtcgatgtt tcggtatagt
     2521 ttacgggtat ctcaacattt atcttaggga tgatcacctc tggtgtagca gtcggtgcca
     2581 cgctgtcagt gctgatgata aggggcgtag cggcagtggc ccggcttggt tgaatgaatg
     2641 gtgcgatgat gacttcattg aaaaacccga agaggaaaat gacaacggca atgagtccca
     2701 tgctcagtcc aaataagagg gattgaagat ggtgcttggc ctgtagcttg ccgccggcac
     2761 tgaccttgtc gcgtatgacg gcttggactt cggttttggt gcgaacatcg cgtagctt
//
LOCUS       DATNGP010000062         4156 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_939825, whole genome shotgun sequence.
ACCESSION   DATNGP010000062 DATNGP010000000
VERSION     DATNGP010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4156
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_939825"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="VN778_01510"
     CDS             complement(<1..264)
                     /locus_tag="VN778_01510"
                     /inference="COORDINATES: protein motif:HMM:NF012895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HXR49685.1"
                     /translation="MKIQLKQFDTSLPVPAYQTVGAAGMDLYARQETTIAPKTIGYVP
                     LNIALEIPEGYWVLVAARGSTHKQGLMPAHGIGIGDWDFRGDND"
     gene            complement(261..767)
                     /locus_tag="VN778_01515"
     CDS             complement(261..767)
                     /locus_tag="VN778_01515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49686.1"
                     /translation="MNTFIRLLYTVLIAAALVLFIGIGITTFYPAPEAPVYPKMLETP
                     AIAQKDGTDNASLRAQQEYDAAYQKYQDATKVYNRSLSIIGTSLAVVVVIAGIAIRRR
                     SDIIGDGVTLGGIATSVYGITTAVLADDRIMRFIAVTVFLASAIVVVYVQFSNQESKT
                     KPAAAKKS"
     gene            878..1798
                     /gene="holA"
                     /locus_tag="VN778_01520"
     CDS             878..1798
                     /gene="holA"
                     /locus_tag="VN778_01520"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HXR49687.1"
                     /translation="MVTVLTGENSFAWGRVLAARLEQFVAVHGDIAVERLDGESASLA
                     RIQESLQSSPFLAARKLVVLRNPSLNKQFIDNLEPTLTAIADTTDLVIVEPVIDKRSS
                     YFKWLKKETDLQEFTELDELGLARWLVAATQEQGGELSAADARLLVGRVGASQQLLAS
                     ELNKLLSYDPHITREAIELLTEPAPQSKVFDLLDAAFAGRAKQALEIYKDQRAQRVDP
                     SQIIAMVAWQLKVLAIVKSAGSRRPSDIAREAKLSPYVVQKSESIAAWVTAARLKELI
                     NDLLVLDLRSKRERIDLDEALQNYLIKLAQ"
     gene            complement(2151..2531)
                     /gene="rpsT"
                     /locus_tag="VN778_01525"
     CDS             complement(2151..2531)
                     /gene="rpsT"
                     /locus_tag="VN778_01525"
                     /inference="COORDINATES: protein motif:HMM:TIGR00029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HXR49688.1"
                     /translation="MPIIKSAKKRVRVAKKAAVRNSKTKRTLRGALKLFSRKASSSAH
                     SSAQSAIAKAAKKGVMHKNKAARLQKQLAAKSRAAGVKPAAAAKKAAPKPAAKKAAPA
                     KKAPAKKATPAKKPAAKKAPAKKK"
     gene            2664..3209
                     /locus_tag="VN778_01530"
     CDS             2664..3209
                     /locus_tag="VN778_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49689.1"
                     /translation="MNTALGRLTTRGEAETNTTPPLSPHTQGVHPQPVTMDDVPVATG
                     FFNRVRLQGIALGYVVLAETAWRLRQIGAGLIIAHNILQSELAARGEEANNAFVDRHN
                     GGAITHSDDLTLHDTAEQMVRHVAARALVRTARIGAHVGNVLWQSDDAMAQAHNEFGD
                     EAANLQAQTRQADKAIPNIKP"
     gene            3261..>4156
                     /locus_tag="VN778_01535"
     CDS             3261..>4156
                     /locus_tag="VN778_01535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49690.1"
                     /translation="MDDIKQQVADKLKQATNILVTVKTDPSIDLLAACLGLSLVLDKV
                     GKHATAVFSGEVPSVMEFLKPEETIEKTPNSLRDFIISLDKAKADKLRYKVEDKVVRI
                     FITPFKAGLSQEDLEFSQGDFNIDLLVALGVRDQAELDAAVTAHGRILHDAAIISINT
                     DTNSNLGTLNWLGTNVSSVSEMIASLAKTLDPNVFDEQIATALLTGIVAETARFSNDK
                     TSPGTMRLAGELMAAGANQQLVATELQSAMSVGAPGSVKADIKPSDDNGTLEIKHEEL
                     PPEEPKTEAKLPEPMPELPKPE"
BASE COUNT          990 a         1021 c         1146 g          999 t
ORIGIN      
        1 gtcattgtcg ccgcgaaaat cccagtcgcc gatgcctatg ccgtgggcgg gcataagccc
       61 ctgtttgtga gtcgagcctc gggcagccac tagcacccag taaccttcgg gaatctccag
      121 cgcaatattg agcggtacgt agccaatagt tttcggtgcg atcgttgttt cctgccgcgc
      181 atataaatcc atgccagccg cgccaacagt ttggtacgca gggacgggaa gcgacgtatc
      241 gaattgcttg agctgtatct tcatgatttt ttggcggccg ctggttttgt cttggattcc
      301 tgattgctaa attggacata gacgacaact atagcgctgg ccaaaaatac ggtcaccgct
      361 atgaaacgca taatgcggtc gtccgccaag actgcggttg tgataccgta gacgctggtc
      421 gcaataccgc cgagcgttac gccgtcgccg ataatgtccg agcgccgcct gatagcgatg
      481 ccagcgataa cgacaaccac agccaggctc gttccgatga tactgaggct acggttgtac
      541 accttggttg cgtcttggta tttctggtat gcggcatcat attcctgttg ggcgcgtagt
      601 gaagcgttat cggtgccatc tttttgggcg attgccggtg tttcgagcat ctttggatac
      661 accggggctt ccggtgcggg ataaaacgta gtgatgccaa tgccgatgaa cagcacaagc
      721 gctgccgcaa ttaaaacagt gtagagtaac ctgatgaatg tattcatagc ctcttcattt
      781 ctacttatgc ttacatcata gcattgatca gtgagccttt accgtcgatt gggaacgtcc
      841 atgcaggagt ggtaaatgtc ctgtatcata cggatacatg gttacggtgc taacgggaga
      901 gaatagcttt gcctgggggc gcgtgctggc agcgcggctc gagcagtttg ttgccgtcca
      961 tggcgatatt gctgtcgagc gtttggatgg cgagtcagcg agtttggcgc gtattcagga
     1021 gtcgctgcag agctcaccgt ttctagctgc ccgcaaactt gtcgtgctgc gaaaccctag
     1081 cctgaacaag cagtttatcg acaatttaga gccgacactt acggctattg cagacacgac
     1141 agatcttgtt atcgtcgagc cggttatcga caagcgttcg agttatttca aatggctaaa
     1201 aaaagaaacg gatctacagg aatttacaga gctggacgag ctaggtctgg cgcgttggct
     1261 ggtggctgcc actcaagagc aggggggcga actcagtgct gctgatgcgc ggctacttgt
     1321 cggacgagtc ggcgccagtc agcagctgtt ggcgagcgaa ctgaacaaat tattgagcta
     1381 tgacccgcac attacccgtg aggcgattga gctgctgacc gaaccggcgc cgcaaagcaa
     1441 ggtctttgat ctgctcgacg ctgcgtttgc tggacgtgcg aaacaagcac tagagattta
     1501 taaagatcag cgggcgcaac gagtggaccc gtcccagatt attgctatgg ttgcctggca
     1561 gctcaaagta ttggccatcg tgaagtcggc tggtagccgc agaccgagtg atattgcccg
     1621 cgaagccaaa ctcagcccct atgttgtaca aaaaagtgaa tcaattgccg cttgggttac
     1681 ggccgcgagg ctgaaagaac taattaacga tctgctcgtg cttgacttgc gttccaagcg
     1741 ggagcgcatc gatctcgacg aagctctcca aaattatctg attaaactgg cgcagtgagc
     1801 cactcgttcc tttccgcgga accaaatcct ctttcttttg ccttgatgca aaagaaacaa
     1861 aaaatcaagg cctggtgctc gcttctcggg tgcagttgta aaagcttaca agcgcctgcg
     1921 gaacgcctca gcaagcactc gcctttggcg acgtgcttgc ttcgttggac ggtcctcggc
     1981 ttggtcttct tgtaactttt agcaacagcc cgccaggagg ctgtgctatg gccaactaac
     2041 ggccgtagat cggttccggc cgctgtttga ttaattagtc aacgaggctc tctggaaagg
     2101 agcaaaaaac cgcctcttgg cgaggcggcc cgaagtgaag caggagctac ttactttttc
     2161 ttggcaggtg cttttttggc agcaggcttc ttagccggag tagccttttt tgcaggagct
     2221 ttcttggctg gcgcggcctt tttagcggct ggtttagggg cggctttctt ggcggcagcg
     2281 gctggtttga cgccagcggc cctggatttg gcggcgagct gcttctgtaa gcgcgcagct
     2341 ttatttttgt gcatcacgcc ttttttggca gctttggcga tagcgctctg ggcgctcgaa
     2401 tgtgcgctgg agctggcctt gcggctaaag agttttagtg cgcctcgcag agtgcgcttg
     2461 gttttgctgt tgcgcactgc agcctttttg gcgacacgaa cgcgcttctt agcggactta
     2521 ataatgggca ttattttttc ctttgttact tctttttaaa agtccccttg aatatagcag
     2581 atatagggcg agcctgcaaa ataacgccca taactataat aataagttga cttagtagct
     2641 ataaaagtat atagtgtggt tagatgaata cagcacttgg acggcttacg actagaggag
     2701 aagcagagac taacaccaca ccgcccttgt ctcctcacac gcaaggcgtg catccacagc
     2761 ctgtaactat ggatgatgtt ccggtagcaa caggattttt caatcgggtt cggttgcaag
     2821 gaattgcgct gggctatgtg gtgcttgctg agactgcctg gcgacttcgg cagataggtg
     2881 ctggactaat cattgcccat aacattttgc agagcgagct ggctgcccgt ggggaagaag
     2941 ctaacaatgc ctttgtcgat cgtcacaatg gcggtgcgat aacacattca gacgatctaa
     3001 cattgcacga tactgcagag caaatggtgc gtcacgtagc agcccgcgcg ttagtaagaa
     3061 cagcacgaat tggcgcacat gttggcaacg tgctctggca atctgatgat gctatggctc
     3121 aggcgcataa cgaattcggt gacgaggcgg caaacctaca agctcagacg cgccaagctg
     3181 acaaggctat tccgaatatc aaaccgtaga caacctattc tgtttatgct aggctagagc
     3241 caaagcctaa cataaacact atggatgata taaaacaaca agttgctgat aagttaaagc
     3301 aggcgacaaa tattcttgtg acggtcaaaa ctgacccgtc gattgatctg ttggcggcct
     3361 gcctgggctt aagtctcgta ttagataaag tcggtaaaca cgctacagct gttttcagcg
     3421 gtgaagtgcc gagcgtcatg gagttcttga agccggaaga gacgattgaa aaaacgccga
     3481 actcgcttcg tgactttatt atctcgcttg acaaggccaa ggccgacaaa ctgcgctaca
     3541 aagtcgaaga caaggtagta cggatcttta ttacgccatt taaagccggt ttgagccagg
     3601 aagacctgga gttcagccaa ggcgacttca atattgacct gctggttgcg ctgggcgtac
     3661 gcgatcaagc cgaactggac gcggcagtca cggctcacgg tcggatcttg catgacgcgg
     3721 caatcatatc gattaacacc gacaccaaca gcaacctagg tacacttaac tggctgggaa
     3781 cgaacgtcag ttctgtttct gaaatgatcg ctagcctggc caagacgctc gatccgaatg
     3841 tatttgatga acagattgcc acggcgctat tgacgggcat cgttgccgag acagctcggt
     3901 ttagtaacga caaaacgtct cctgggacga tgcggctagc cggtgagctc atggcggccg
     3961 gcgccaacca acagctggtg gcgacggaac tccagagtgc gatgagtgtt ggtgcgccgg
     4021 gaagtgtaaa agccgacatt aagccatcgg acgataacgg cacgctcgag attaagcacg
     4081 aggaactccc gcccgaagaa ccaaagactg aagccaagct gcccgagcca atgccagaac
     4141 taccgaagcc ggaaat
//
LOCUS       DATNGP010000063         3461 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_1705974, whole genome shotgun sequence.
ACCESSION   DATNGP010000063 DATNGP010000000
VERSION     DATNGP010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3461)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3461
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1705974"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..278
                     /locus_tag="VN778_01540"
     CDS             <1..278
                     /locus_tag="VN778_01540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49691.1"
                     /translation="RAVPFRPTPPVLWDVGVGYRTWHLWLEHFLPALGALIQRRLHDS
                     LDRRRAGAQEAIPQTISPVRRPHRHVRPAYKEEKLRSTLCVLIIMII"
     gene            419..1354
                     /locus_tag="VN778_01545"
     CDS             419..1354
                     /locus_tag="VN778_01545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49692.1"
                     /translation="MDQLTLRRPQASKPGGLGSMRRQFTKKTLRTKRRLIAAIRRQYL
                     LTHALSDVKAAALGSEDITFGALMMAAVVSYALLTTLANLLFIFMQTTAAFSTWSGIS
                     LGLLVMITLGIFISLSVWILAWGQNMLSISILESANRKQNRSIRTTIRHGLRSATRVT
                     LAWLLVNVLTFAPAAVLGLILLGVLYASHVALSAALPYLFGVGALGIIWIGYAVANFA
                     LTPKVALFEPKLSLAESLGRSRQLLKSKGRVFLIGNYVALLATLALAYGLSNVLQWFT
                     GVSNVLSFIIFAWVLFSLNNLIMSAFYRKRKLARK"
     gene            1413..1775
                     /locus_tag="VN778_01550"
     CDS             1413..1775
                     /locus_tag="VN778_01550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49693.1"
                     /translation="MLVVAFFSWWYGAGWRQVAASFEARLHGVAEAFSVRQLLSTLFA
                     PWRRIITYPGASLGERLRAWSDNVFSRTIGFVVRVFVLLAALLLTLVVALLTIIEIVL
                     WPLAPLAIPCLIVAGLLA"
     gene            1772..>3461
                     /locus_tag="VN778_01555"
     CDS             1772..>3461
                     /locus_tag="VN778_01555"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXR49694.1"
                     /translation="MSQLFDLRSRRAAEARFGRAIGSRGYRALLITGSILLAAAIVQG
                     MTHGIRYTYLLLSPALLCYTVAVWWKRQLAVLPPSGQDLTGRLSVDVLARLKPDMTLQ
                     PQNLWLALHDHWQAFFITNHLLITREMAHAQLSTNEADLDQALQIATHLAERNGNATI
                     ELGFVIAGLLMTSPSMQQLLTQIKAQPSDIEVTADWLGRNLSDMHAVKRNFGGIGRDW
                     AFGFTPLLNRFGQNISLAIAKHNAHFGWLVKSESVRSIETALSHKNAAVALVGPDGIG
                     KTTSVYALAQRLIEGDTAQTLAYHQVISLNATDLASRARGPGDLKHIMVSLANEAAHA
                     GHIILLFDNAQMFFGDGHGSFDASQILLSIVEARSVPMIFALSPQDFQRLKSRSTSLT
                     AQLTPVVLQELPETDVMRVLEDTAVGLENRHHILVAYEALREAYRLSGRYEQDEAYPG
                     KAIKLLEQAVNYAEQSVTAASVQRAVEQTYGVKASSAAPAEADALLHLEDAIHARMVN
                     QTHAVKAVASALRRARAGVTNPRRPIGSFLFLGPTGVGKTELARSIAATYFGAES"
BASE COUNT          734 a          947 c          927 g          853 t
ORIGIN      
        1 agcgggccgt accgtttcgt ccgacacccc ctgtactctg ggatgttggt gtcggctatc
       61 ggacttggca tctttggctg gaacattttc ttcctgctct cggcgctctc attcagcggc
      121 gcttacacga ttcgcttgat aggcgaagag caggagctca agaagcgatt ccccaaacaa
      181 tatccccggt acgccgcccg cacaggcatg ttcgtcccgc gtataaggaa gagaaattaa
      241 ggagtacgct ctgcgtcttg atcattatga tcatttaaat tacgcagagc ttctccgtgt
      301 actacgacac ttaccataca ataacattgc aatataatta ctattgtgct ataattatct
      361 ccaaagttgt aattctcggt gggactagcc gagcgagcag gagatacgag cagtgatttt
      421 ggaccaatta acactccgcc ggccgcaggc ctcgaaacct ggcggtctgg gatcaatgcg
      481 gcgtcaattt actaagaaaa cgctgcgaac aaaacgcaga ctcatagcgg ccatacgccg
      541 acagtatctt ttgacgcacg ccctgagtga cgtgaaggca gccgcactgg ggagcgagga
      601 cattaccttc ggcgctttga tgatggccgc ggtggtcagc tatgcgctcc tcaccacgct
      661 agccaatctc ctgtttatct ttatgcaaac aacggcggct ttttctactt ggagcggcat
      721 tagcctcggc ttgcttgtga tgattaccct tggtatcttt attagcctgt cagtttggat
      781 tctggcttgg gggcaaaata tgctctcgat tagcatcctg gagagcgcca atcgtaagca
      841 aaatcgttcc atacggacta ccatacgcca cggactccgg agcgccaccc gcgttacttt
      901 ggcttggctg ctagtcaatg tgcttacttt tgcgccggcc gccgtgcttg gcttgatttt
      961 gctgggcgtg ttgtacgcca gccacgtcgc gctgtctgca gctctgccgt atctctttgg
     1021 tgtaggtgcc ctcggtatca tctggatcgg ctatgcagta gcaaactttg ccctaactcc
     1081 aaaagtagcg ttgttcgagc caaaactgag tttagcggaa agcctgggcc gcagccgtca
     1141 attattgaag agtaaaggcc gcgtattttt gattggcaat tatgttgctt tgctggcgac
     1201 gctagccctg gcgtacggtt tgtctaatgt gctgcagtgg ttcacagggg tcagcaatgt
     1261 cttgtctttc atcatctttg cttgggtgct tttctcgctc aacaatctga tcatgtcggc
     1321 tttttatcgc aagcgtaagc tcgcccgcaa gtaatagccc gccgacaatt aacaggccga
     1381 cacatcccct gacaagtgtt acaatgttgg ttatgcttgt ggtagcgttt ttttcgtggt
     1441 ggtatggagc aggctggcgg caggttgcgg ctagttttga ggcgcggtta cacggagtag
     1501 ccgaagcgtt ttcagtccgt cagctgctga gcaccttatt cgcgccctgg cggcgtatta
     1561 ttacatatcc gggcgcaagc ttgggggaac gcttgcgggc ctggtcggat aatgtattta
     1621 gtcgaactat tggctttgtg gtacgtgtgt ttgtcctgtt ggctgcgcta ttacttaccc
     1681 ttgtggtggc actcttaacg atcattgaga tagttttgtg gccgctagca ccgctggcaa
     1741 ttccatgcct tattgtagcg gggttactcg catgagccag ctgtttgatc tcagaagccg
     1801 gcgggccgcc gaagcccggt tcggcagagc gattggcagt cgaggctatc gggctctctt
     1861 gattaccggc agcatcttgc tggccgcggc aattgtgcag ggcatgacgc atggaattcg
     1921 ctatacctat ctcttgctgt cgccggcgtt gctttgttac acggtcgcgg tttggtggaa
     1981 gcggcaatta gccgttttgc cgcccagcgg ccaggatcta acaggtcgac tatcggtgga
     2041 cgtgctcgcg cggctcaagc cggacatgac actgcagccg caaaatctgt ggctggccct
     2101 gcatgaccac tggcaggcat tttttattac gaaccatttg ctcataactc gtgaaatggc
     2161 ccacgcccag ctgagcacca atgaagccga cctcgaccag gccctgcaga ttgccaccca
     2221 cctggccgaa cgtaatggca atgcaaccat cgaacttggt ttcgtaattg ccggtttact
     2281 catgacatcg cccagcatgc agcaactttt aactcaaatc aaagctcagc ctagcgacat
     2341 cgaagtaact gccgactggc tgggccgcaa cctcagtgat atgcacgcgg taaaacgcaa
     2401 ttttggggga atcggccggg attgggcctt cgggtttacg ccgctgctca atcgctttgg
     2461 gcaaaacatc agcctggcaa ttgccaaaca caacgcgcac ttcggctggc tcgtcaaatc
     2521 agagagcgtc cgatctatcg agactgcact gagtcataaa aatgctgccg tggccctcgt
     2581 cggtcctgac ggtatcggca aaactacgag tgtctatgcg ctggcccaac gtttgatcga
     2641 gggtgacacc gcccaaacct tagcctacca ccaggtcatc tcgttgaacg ccaccgacct
     2701 tgcctcgcga gcgcgcgggc ccggtgattt gaaacacatc atggttagcc tggctaacga
     2761 agctgcccat gctggccata tcatcctctt atttgacaac gcccagatgt ttttcggcga
     2821 cgggcacggc tcgttcgacg ccagtcaaat tcttttatca atcgtcgaag cccgctccgt
     2881 accgatgatt ttcgcgctgt cgccgcaaga ttttcagcgt ctgaaatcac gtagcaccag
     2941 cctaacggcc caactcacac ccgttgtgct gcaagaattg cccgaaacag atgttatgcg
     3001 ggtacttgaa gacactgccg tcgggctgga aaatcgccac cacattctgg tcgcctatga
     3061 agcgctacgc gaagcatatc gcctgagtgg gcgctacgag caagacgaag cttaccccgg
     3121 caaagcgatt aagctgctcg aacaagctgt taactatgcc gagcagtcag tcacagcagc
     3181 gagcgttcag agagccgtcg agcaaaccta tggtgtcaaa gccagcagcg ctgcgccggc
     3241 tgaagccgat gcgttgcttc atttggaaga cgccattcat gcgcggatgg ttaatcagac
     3301 tcacgccgtc aaggcagtgg ctagcgctct gcgccgggcc agggcagggg tcaccaaccc
     3361 gcgccgtccg attggcagtt ttcttttcct cggtcccaca ggtgtcggca agacagagtt
     3421 agcgcgctcc attgccgcga catacttcgg cgccgaaagt g
//
LOCUS       DATNGP010000064         3057 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_2873973, whole genome shotgun sequence.
ACCESSION   DATNGP010000064 DATNGP010000000
VERSION     DATNGP010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3057
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2873973"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..187)
                     /locus_tag="VN778_01560"
     CDS             complement(<1..187)
                     /locus_tag="VN778_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011936249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HXR49695.1"
                     /translation="MSGHSKWSTIKRQKGAKDAARGAIFTKLGNAIAIAARGGTDPET
                     NFSLRLAIDKAKTANMPA"
     gene            complement(211..1647)
                     /locus_tag="VN778_01565"
     CDS             complement(211..1647)
                     /locus_tag="VN778_01565"
                     /inference="COORDINATES: protein motif:HMM:NF015714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HXR49696.1"
                     /translation="MRKYRIRRTWLWLIAFMALITGLGLARTGYRFSGLWCGVALGLL
                     FASLCRRSLLTAILVVAFGISVGWWRGSQYLDKLAGYQGLYHRKLTITAQALTDGVYG
                     AHSQISFNASKIRLPDGETLKGQVQISGFGLNSVLEGDEVQVSGKLYPGYGAYQARLS
                     FARLQLIRHHPSLVAEIRRRFAAGMQSALPEPLASFAMGLLIGQRTTLPATVKQDLLM
                     VGLTHIIAVSGYNLTIILRASRGLLAQHSKRLSLAFSVSLTGTFLLLSGLSASIVRAA
                     IVSMLSIYASYYGRSFKPLNLILFAAAVTTLANPVYLWSDISWYLSFLAFFGVMIVSP
                     LLAARFPHRLHNSIIFQVALESVCAEFMTVPIILYAFGQMSFIGLVANVLVVALIPLA
                     MLLTAVAGLSGMLAGAIAGWVSWPARLLLTYMLDVAHALARLPHIFVQNVSISLLQML
                     FLYAVVLLLVAGLWFKSKTKSGMITDKI"
     gene            1847..2101
                     /locus_tag="VN778_01570"
     CDS             1847..2101
                     /locus_tag="VN778_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017908994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HXR49697.1"
                     /translation="MFIILGGIVGWIASRLLGREEGILASIVIGIIGAFIGGIVARLF
                     NSGTAYLTLSWSGFFWSLIGAIILVALLNAFSHPRQHHVQ"
     gene            complement(2404..>3057)
                     /locus_tag="VN778_01575"
     CDS             complement(2404..>3057)
                     /locus_tag="VN778_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007915668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49698.1"
                     /translation="DFLVFHFNPYLAVIGGFTAFVAVLALQFKTRRYVPWIYWLNVLM
                     VAIFGTMAADVTHVVLGVPYLVSTVAFAIVLAIVFIFWQRTEKTLSIHSITTTRREVF
                     YWATVLATFALGTAAGDLTAYTANLGFLSAGILFAIIFALPGLGYWLLRINAIFAFWF
                     AYILTRPLGASFADWTGKTQSVGGLGWGDGPVAGVLALLIIVLVGYLQVTHEDVDAA"
BASE COUNT          855 a          839 c          725 g          638 t
ORIGIN      
        1 cggccggcat gttagccgtt ttggctttat cgatagccaa gcgcaaggag aaattggtct
       61 caggatcggt gccgccgcgt gccgcgatag cgatggcatt gccaagcttg gtgaaaatgg
      121 cgccgcgcgc cgcgtcctta gctccttttt gtcgcttaat cgttgaccat ttgctatgac
      181 cgctcaaacc tcttgctcct tacacttatt tcaaatctta tctgttatca taccactttt
      241 cgtcttactt ttaaaccata gaccagcgac caaaagcagc acaaccgcgt agagaaacaa
      301 catttgaagc agcgaaatac tcacgttctg cacaaagatg tgcggtaacc ttgcgagggc
      361 atgagccaca tcaagcatat aggtcagtag taagcgcgcc ggccagctta cccaaccggc
      421 aatcgctccc gccagcatac cgctcagtcc ggcgactgca gtcagcagca tggccagagg
      481 aatcaaagcc accaccagca cattcgccac cagaccaata aatgacatct gcccgaatgc
      541 ataaaggatg atcggcaccg tcatgaattc cgcgcagaca ctttccagag ctacctgaaa
      601 tatgatcgag ttgtgcaggc ggtgcggaaa gcgagcagcc agcagcgggg agactatcat
      661 gacaccgaaa aaagccagaa agctgaggta ccagctaata tcactccaaa ggtaaacagg
      721 gttcgccaag gtcgtaaccg cggctgcaaa caagattagg ttcaagggtt tgaaacttcg
      781 tccgtaataa ctagcataaa tacttaacat gctaactatc gctgctcgca caatggatgc
      841 actcagtccg ctcaatagca aaaaggtccc ggtgagactc acagaaaatg ccaaggatag
      901 acgcttggag tgctgagcca acagcccgcg gctggcccgc agaataattg tcagattata
      961 gcccgacaca gcgataatgt gcgtcaagcc caccatcaag agatcctgtt tcacggtggc
     1021 cggcaaagta gtccgctgcc ctatcaacaa acccatagca aacgaggcca gcggttccgg
     1081 taaagcactc tgcataccag ctgcgaaccg acgacggatt tctgccacca gcgacggatg
     1141 atgccgaatg agttgtagcc tggcaaaact caagcgtgcc tgataggcac cgtaccccgg
     1201 atacaacttg ccgctcacct gcacctcatc gccttcaagc acactattca gaccaaaacc
     1261 gcttatctga acctgtccct ttagcgtttc gccatcgggg aggcgaattt tgctggcatt
     1321 aaagctaatt tgcgaatggg cgccgtacac tccgtcggtc agcgcctgcg cagtaattgt
     1381 cagctttcgg tgatacaacc cctgatagcc ggctaatttg tctaagtatt gtgagccccg
     1441 ccaccagcca acgcttatgc caaaggcaac aactaagata gctgttaaaa gtgatcgcct
     1501 gcaaaggcta gcaaacaata gacccaaggc gacaccacac catagcccac tgaaccgata
     1561 gcctgtccgg gccaagccca agccagttat tagcgccata aaagctataa gccacaacca
     1621 agtacggcga atccgatatt tccgcatgcc ccgcattcta gcaactcaaa ttgctagcgc
     1681 tcttcaaaat taagaattat ttcatacttc aaaataggtt caacccacca ggggtaaaag
     1741 tccgcccata gtgagatagc tcatagtggc ctattcgact ttacccttat gctgggagtg
     1801 taaataaggc gtgtaaacgt aaaggaggta acgcatgagt atcataatgt tcataatcct
     1861 aggtggtatc gtgggctgga ttgcatcacg tttactggga cgggaagaag gaatactggc
     1921 gagcatcgtt atcggtatta ttggggcctt tattggtgga atcgtcgccc gcttatttaa
     1981 cagcggtaca gcttacctca ccttgagttg gagcggtttc ttttggtcgc tgatcggcgc
     2041 catcatatta gtcgctctct tgaatgcctt ttcacatccc cgacagcatc acgtacagta
     2101 aatatcatga gctcaggtga caaaggtgct ccaaaacggg gcacctttta actacttaac
     2161 gactgccccg atggcgacca gcgcttccat gaaaccccat gagagactcc ctgcaataat
     2221 agctttatct ccctgcctat agccgtaaac acaccagaca cactcaatca aacagtagag
     2281 atccaggtaa tttccgaaat gcctgcagtt tggcctttaa cccacacctg atagacctgc
     2341 ggcagggtgg tgagcgattc cacaaccccc aaaactagta caaagcgatc gaaagcgcta
     2401 cgtttaggct gcatcgacat cctcgtgggt aacttgtaag tagccgacca gcactatgat
     2461 aagcaatgcc agcacgcctg ctaccggccc gtcgccccac cctaagcccc cgacactctg
     2521 cgtcttgcca gtccagtcgg caaacgaagc tccgagcggc cgtgtcagga tgtaagcgaa
     2581 ccaaaaggca aatatcgcat tgatacgaag cagccagtag ccaaggccgg gaagcgcgaa
     2641 tatgatggca aacagtatgc cggccgataa gaagccgaga ttggcagtat aggctgtcag
     2701 atcgcccgca gcggtaccaa gcgcaaaggt cgccagtacg gtcgcccaat aaaacacttc
     2761 gcgccgagtg gtcgtgatgc tatgtatcga caaggttttt tcggttcttt gccagaatat
     2821 gaaaacgatg gccagcacga tagcaaaagc gaccgtggac acgaggtatg gcacacccag
     2881 cacgacatgc gtcacatcgg cggccatggt gccgaaaatc gccaccatca agacgttcag
     2941 ccagtagatc caagggacgt aacggcgagt tttaaattgc agtgccaaga ccgccacaaa
     3001 agcagtaaat ccaccaatga cagcaaggta tgggttgaag tgaaaaacta gaaagtc
//
LOCUS       DATNGP010000065         3261 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_1928133, whole genome shotgun sequence.
ACCESSION   DATNGP010000065 DATNGP010000000
VERSION     DATNGP010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3261
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1928133"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            167..448
                     /locus_tag="VN778_01580"
     CDS             167..448
                     /locus_tag="VN778_01580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49699.1"
                     /translation="MERSHSRYGQTDVWDKIWRDRRGRVVLFQMPNVWLIAWAVLDVL
                     SIFTNGTFSNIVWWLATVTLGIWAILEILQGVNYFRRGLGVVVLIFVLL"
     gene            560..1633
                     /locus_tag="VN778_01585"
     CDS             560..1633
                     /locus_tag="VN778_01585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49700.1"
                     /translation="MAGEVLRPTAKTLNETFTETLQFWNGYFATQSSEACKNMANEAV
                     SQLDQLLAWQGVLHEPCVVNGQGIWSEAAVEVEHGDLEELAEDEANMRMIFNLSVKRG
                     ELKGLEGMCRGFIVLGASVLERPELYYLFETTGWQMAGHGTFTLEGQHRAYVKPENST
                     IVPVSDFETPFVHYPNGTVLDMFSPQAVINSASGELMRLVSSTGFRRQKRQHQEQAID
                     AIIQQAETKSLIKEANMLLEPQYGYIGELTASGEMDYIRLALGGLCVEGTCLGLTMLG
                     KERLSHKAIRRDTDVVNKRDGISMSIEVKEPLLLNDRIVSADSIVHIPLSGQDFECII
                     ELNEELYTITSGEGPGQTEASPA"
     gene            complement(1634..2161)
                     /gene="msrA"
                     /locus_tag="VN778_01590"
     CDS             complement(1634..2161)
                     /gene="msrA"
                     /locus_tag="VN778_01590"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018464977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HXR49701.1"
                     /translation="MSKLATLGGGCYWCLEAFYQRVKGVKQVMSGYSGGQLDNPTAER
                     VYQGGTGHAEVVQLTFDPSIITYREILEIFFVMHDPTTLNRQGADVGEIYRSVIFYRD
                     DAQKQIAEEMTKGFAAELWPDPIVTQLVPFEKFWPADDYMQNYYNNNPEAAYCQIVID
                     PKIQKLRQKFAAKLQ"
     gene            2198..2821
                     /locus_tag="VN778_01595"
     CDS             2198..2821
                     /locus_tag="VN778_01595"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HXR49702.1"
                     /translation="MAAIIFDFDGTIADSFETVVQIFHELTGRKERLSPEAIERLRGM
                     SLPQAAEELGVRPWRMPFLVMVGRRRMGKRIAQVRTHPGVPETVRKLYAEGHQLFIMS
                     SNSEKNIRLFLKQHHMLKEFVSIYGNVGLLSKAGVLRKIRRRNRLGSDTCWYVGDEVR
                     DIEGAKLAGIKVIAVTWGFNTAELLRGHQPTVLVNAPTELLQVLEEA"
     gene            2821..>3261
                     /locus_tag="VN778_01600"
     CDS             2821..>3261
                     /locus_tag="VN778_01600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49703.1"
                     /translation="MSEEPQTINYQAYDNMHHASKAWFLLAVPCVGAFIALLVVAILQ
                     ANQPQYGSSGVSGLAGPVFLTFFVLLIGTVLIYAWRQSKYLDAAFSQFLLDNSFVPET
                     GGMDMVATSLLGIGDSQKVLLAFTGQYQGRTMHGVIYQYKTGNGK"
BASE COUNT          786 a          746 c          900 g          829 t
ORIGIN      
        1 agcacgttag atgagcttaa ggaacagatt aggcatacac tttggcacga gattgcccat
       61 tactacgggc tcaaccatga ccgtatccgc gcgctggagt aaaaggttcg ttttaccggc
      121 gatcagactt cctttatact agaaagaaca taatttggag ggcagcgtgg agcgaagcca
      181 cagtcgctat gggcaaactg atgtttggga taagatttgg cgagaccggc gcggccgggt
      241 agtgcttttc caaatgccta atgtctggct cattgcctgg gcggtgctcg acgtgctgtc
      301 gatcttcacc aacggcactt tcagcaatat tgtttggtgg ctggcgaccg tgactttggg
      361 tatctgggca atactggaaa tacttcaggg agtgaactat tttcgccgtg gcctgggcgt
      421 cgttgtcttg atctttgttc ttctgtgatc tttgttcttc tgtctatttt caaggtcggc
      481 ctctaaaccc ccgagctagt gccgggcgct atttaacaaa aaagttgctc gtcggccaag
      541 tatacactat gctaagccta tggccggcga ggtgttacgc cctacagcaa aaactcttaa
      601 cgaaaccttc actgaaacgc tgcagttctg gaatggttac tttgcgacgc aatcgagtga
      661 agcgtgcaaa aatatggcta acgaagctgt aagccagctc gatcaactgc ttgcgtggca
      721 aggcgtgttg catgaaccct gcgtcgtaaa cggccaaggc atttggagtg aggctgccgt
      781 agaggtggag catggtgatc tagaagagtt ggcagaggac gaagccaata tgcgcatgat
      841 cttcaatctc agcgtcaagc gtggcgagct gaaagggctg gaaggtatgt gtcgcggctt
      901 tattgtactg ggagcttcag tacttgaaag gcccgagctg tattatttat ttgaaaccac
      961 aggttggcag atggctgggc atggtacttt tacgcttgag ggtcagcatc gggcttacgt
     1021 gaagcccgag aacagcacaa ttgtcccagt atcggatttt gaaacgccct ttgtgcatta
     1081 cccaaatggt actgttctgg acatgttctc acctcaggcg gttattaact cggcttctgg
     1141 cgagctaatg agattggttt cctcaacggg ttttcgtcgt caaaaacgcc aacatcaaga
     1201 acaggcaatc gacgctatca ttcagcaagc agaaactaag agtctgatca aagaagccaa
     1261 tatgctgcta gagccacagt acggctatat cggtgaactg acggcaagcg gagaaatgga
     1321 ctacattcgg ctggcgctgg gaggcttgtg cgttgaagga acatgtcttg gcctgactat
     1381 gctgggtaaa gaaaggttat cacataaggc catacgccga gacactgatg tcgtcaataa
     1441 gcgtgatggt atctcgatga gtattgaagt caaagaaccg ctgctcttaa atgatcgtat
     1501 agtctctgcc gacagcattg tgcatatacc gctgtcaggg caagattttg agtgtattat
     1561 tgagctcaac gaagagctct acacaattac cagtggcgag ggccctggtc agacagaagc
     1621 cagcccggct tagttactgt agtttggcgg cgaatttttg gcgtagtttt tggattttgg
     1681 ggtcaatgac gatttggcag taggcggcct cagggttgtt attgtagtag ttttgcatgt
     1741 agtcgtcggc aggccagaac ttctcgaaag gcacgagttg ggtgactatt ggatcgggcc
     1801 ataattcagc ggcaaagccc ttggtcatct cttcggcaat ctgcttctgc gcgtcatcgc
     1861 gataaaaaat gaccgatcgg tagatctcgc cgacgtctgc accctggcga ttcagggtag
     1921 tgggatcgtg cataacgaag aaaatttcca gaatctcgcg gtaggttatg attgaggggt
     1981 caaaggtcag ctgcaccact tcggcgtggc cggtacctcc ctgataaaca cgttcagccg
     2041 tcggattatc aagctggccg ccactataac cggacatcac ctgcttaacg ccttttacgc
     2101 gttggtaaaa agcttccaga caccagtaac agccgccgcc taatgttgct aatttactca
     2161 catatttatc ttagcagttt tcgggtacac tgtagccatg gctgccatta tctttgattt
     2221 cgacggtacg attgccgaca gcttcgaaac tgtcgtccag atattccatg aactgaccgg
     2281 ccggaaagag cggctttccc ccgaggcaat tgagcgcttg cgcggcatgt cgctgcctca
     2341 agcggccgaa gagttaggcg tgcggccgtg gcggatgccg tttttggtaa tggtcgggcg
     2401 gcgccggatg gggaagcgga tagcccaggt gcggactcat cccggggtgc cggaaactgt
     2461 ccgcaagctc tatgccgagg gtcaccagct ctttattatg agctccaata gcgaaaagaa
     2521 catccgcttg tttttgaagc agcatcatat gctgaaagag tttgtcagca tctatggcaa
     2581 tgttggcttg ttgagcaaag ctggagtgct gagaaaaata cgccgccgca accggttagg
     2641 gtcagatacc tgttggtacg tcggcgacga agtccgggac attgagggtg ctaagctggc
     2701 cggcattaaa gttattgctg tcacctgggg atttaatacc gccgagctgt tgcgcggcca
     2761 ccaaccgaca gttctcgtga atgcaccaac tgagctattg caggtgctgg aagaagcgta
     2821 atgtcagaag aaccccaaac aattaattat caagcttatg ataatatgca tcacgcatca
     2881 aaagcttggt tccttctggc ggtaccgtgt gtaggagcat ttattgcact tcttgtcgtt
     2941 gctatattgc aagccaatca gccgcagtac ggatcaagtg gtgtaagcgg cctggcaggc
     3001 ccagtgttcc tgaccttttt tgtcctattg ataggaaccg tactcatata cgcgtggcgc
     3061 cagtccaaat acctggatgc ggcttttagc cagttcctgc ttgataatag ctttgtgcct
     3121 gaaacaggcg gcatggatat ggttgctacg tcattactgg ggataggtga tagtcaaaag
     3181 gtgctgctgg catttaccgg gcagtaccag gggcgaacaa tgcacggggt tatttatcag
     3241 tacaaaactg gcaacggcaa g
//
LOCUS       DATNGP010000066         3574 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_3832472, whole genome shotgun sequence.
ACCESSION   DATNGP010000066 DATNGP010000000
VERSION     DATNGP010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3574
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3832472"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            91..166
                     /locus_tag="VN778_01605"
     tRNA            91..166
                     /locus_tag="VN778_01605"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:124..126,aa:Trp,seq:cca)
     gene            310..630
                     /locus_tag="VN778_01610"
     CDS             310..630
                     /locus_tag="VN778_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010076327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HXR49704.1"
                     /translation="MDNITEMLKGVLEGCVLKIIDRQPTYGYDITQQLRKLGFSDVVE
                     GTVYTILVRLEKNNLVSIEKRPSEVGPPRKFFRLTSAGENELELFWEKWDFVASKINE
                     LRSE"
     gene            631..960
                     /locus_tag="VN778_01615"
     CDS             631..960
                     /locus_tag="VN778_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013487677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1048 domain-containing protein"
                     /protein_id="HXR49705.1"
                     /translation="MKIINSVMGDLKEKKEYKLIEKRAKALPSEYATAYQEIKHYLWN
                     TSGILTVGPLASLVDMLEEAAANGKHVLDITGPDVAAFADELVRGESSYKDQQRQKLN
                     SKLNKKG"
     gene            967..1899
                     /locus_tag="VN778_01620"
     CDS             967..1899
                     /locus_tag="VN778_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004629685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXR49706.1"
                     /translation="MAEAAVQVKDLHKSFKTNKVLKGVNFEVAKGTIFALLGSNGAGK
                     TTTINILTTLLPADQGTAVVNGFDVAKQPARVRASLSLTGQFAAVDDVLTGRENLILI
                     ADMRHAGNSAEIASALLKKFDLVDAADRRVQTYSGGMRRRLDIAMSLIGNPPIIFLDE
                     PTTGLDPQARTAMWQTIRTLANSGTTILLTTQYLDEADELADTIALLHQGVIVASGTP
                     DELKKLVPGGLVTLSFHNKAQLEAATEVLADYKITDSKDLTLTLAIGESIQALTNLFI
                     HLRDAHIEPADFAQKNPSLDDVFFKIVGHKKEGN"
     gene            1902..2669
                     /locus_tag="VN778_01625"
     CDS             1902..2669
                     /locus_tag="VN778_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003766270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXR49707.1"
                     /translation="MKALKDTWTMTKRSLRHIVRSPDTIITVVITPIALLLLFVYVFG
                     GALGRQTGSVSYVNFLTPGILIMTVISGIAYAAYRLGADIQRGIINRFRTMPVSPSSI
                     LGGQALSSTLSNLFSSLLVLAVACGIGFRSGAGPTQWLIFTALIALFTLATTWMAIFF
                     GLLAKSAEGAGSFSYILMLLIFISPSFVPTSSMASALRGFAETQPLTPIIETMRSLLI
                     NGSAGSKAGIAFAWCVGILVLFYLASIRLYRRKTVGA"
     gene            complement(3059..>3574)
                     /locus_tag="VN778_01630"
     CDS             complement(3059..>3574)
                     /locus_tag="VN778_01630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HXR49708.1"
                     /translation="MDYQGSVADHNYLYSEPHAENVSYTHSTKKIELTNLQRDIVNLL
                     TKRPTLSLLDMAKRLEKTYDTVQSHYRSLLATGVLLKCVPIINHELLGYTNTLCFYTL
                     SPKPARINAFWETCRAHPNIVRQSRCLGTFTIVLNIHSQTEHEFKAITSTLNKEFSDC
                     IAEYTALHTID"
BASE COUNT          955 a          801 c          848 g          970 t
ORIGIN      
        1 atatagtggg tgcaagccca gcccagcaag aattcgtaca gttttattga ggtttttacg
       61 ctatttcagg tataattcga cttgatattt aggggagtag ctcagttggt agagcaccgg
      121 tctccaaaac cgggtgtcgc aggttcaagt cctgtctccc ctgccaggaa ttatacattt
      181 cgtcaactcc atggttggcg attttatttt attgctgttg attcttatgc acaagctcat
      241 tgacatttat agctactacg tgctactata tagcagtagt aagtaacact gcatagtaaa
      301 ggaacatatt tggataatat tacagaaatg ctaaaggggg tacttgaagg gtgcgtactt
      361 aaaatcattg accgccagcc gacgtacggc tatgacatta cccagcaatt acggaaacta
      421 gggtttagtg atgttgttga aggaacggtc tacaccattc ttgtccggct cgaaaagaac
      481 aatcttgtta gtatcgaaaa aaggccttct gaagttgggc cgcccagaaa attctttcgc
      541 ctaactagtg cgggcgaaaa cgaactagag cttttttggg aaaaatggga ttttgtagca
      601 tcaaaaatta atgaactaag gagcgaataa gtgaagatta ttaactcagt tatgggtgat
      661 ctaaaagaga aaaaggaata taaactaatc gaaaagcgcg ctaaggcact gccgagcgaa
      721 tacgcaaccg catatcagga aattaagcat tatctttgga acacatctgg catattaacc
      781 gtagggccac ttgcatcact cgttgacatg cttgaagagg cagctgcaaa tggtaagcac
      841 gttctggaca tcaccggtcc ggatgttgcc gcatttgcgg acgaactggt acgcggcgaa
      901 agctcataca aagatcagca acgccaaaag ctgaatagca agctaaacaa aaagggataa
      961 gaaactatgg cagaggcagc agttcaagta aaagaccttc acaaatcttt caagacaaat
     1021 aaggttctga agggggttaa ctttgaagtt gccaagggta cgatatttgc cctgcttgga
     1081 tctaacggcg caggcaaaac gactaccatt aatattctga ccacgcttct gccggctgac
     1141 caagggacag ccgtcgttaa cggatttgac gtagcaaaac agccggccag agtgcgtgct
     1201 tctcttagcc tcacggggca atttgcagca gtggatgatg tgctgaccgg acgagagaat
     1261 cttatcttaa tcgccgacat gcgtcatgct ggtaactcag cagaaattgc atcagcatta
     1321 cttaagaagt tcgatttggt ggatgccgca gaccggcgtg tacaaacata ttcagggggc
     1381 atgcgtcgca ggctagatat tgcgatgagt cttattggaa accctccgat tattttcctg
     1441 gacgagccaa ccaccgggct cgaccctcag gctcgcactg ctatgtggca aaccatcagg
     1501 acgctagcaa atagcggcac gacaatcttg ctgaccactc agtatctcga cgaggccgat
     1561 gaactggcgg acacaatcgc cttgttacat caaggtgtga ttgttgctag cggcacgcct
     1621 gatgaactta agaagcttgt gccaggtggt ctcgttacgc tgtctttcca taacaaggca
     1681 cagctagaag ctgctacaga agtcttagca gactacaaaa ttactgacag caaagattta
     1741 acactaacat tggcgatagg tgaaagcata caagcactca ccaacctctt catccacctg
     1801 cgtgacgcac acatcgaacc tgctgacttt gcacagaaga acccatcgct tgatgacgtt
     1861 ttctttaaaa ttgtaggcca taagaaagaa ggtaactaat aatgaaagcc ttaaaagata
     1921 cctggacgat gacaaagcgc agcttgcgcc acattgttcg cagcccagac accattatca
     1981 cagttgtcat aacaccaatc gccctactgc tgctttttgt ctatgtattc ggcggcgcgc
     2041 ttggccggca aacgggttct gtgagctatg ttaattttct gacgccgggc atacttatta
     2101 tgacggtcat cagtggcatc gcctatgccg cttatcgtct tggcgctgat atccaaagag
     2161 gcatcatcaa ccgattccgg acgatgcccg tctcgccctc gtcaatcttg ggcggccaag
     2221 ccctctcgtc taccctgtcc aacctctttt cttccctatt ggtccttgcc gtagcatgtg
     2281 gcatcggttt tcgctctggt gccgggccta ctcaatggct tatctttact gcgctcatcg
     2341 ctctttttac cctagcaaca acatggatgg ctattttctt tgggttgctc gcaaagtcgg
     2401 ccgaaggcgc aggctcattt agttatattt taatgctgtt gatatttatc agcccttcct
     2461 ttgtgccaac aagctctatg gcctctgcac tacgcggttt tgcagaaacc cagccgctta
     2521 ctcccatcat cgagaccatg cgatcgctgc tgataaacgg gtcggcggga agcaaggcgg
     2581 ggatagcatt tgcctggtgt gtaggtatac tggttctttt ttacctcgcg tcgatccgat
     2641 tatatcggag gaagacagtt ggagcttagc cctattctat cttgataaat aaactgagct
     2701 ccggtaggca caagtattgg tgtgctaaac gccttgcggg gatagaagta tgacgcgagt
     2761 atattaagct ataccttcgt atcactatct tcataggcca tatccaattc ggcaggatcg
     2821 aagtctagct catccgaaaa agttatattc ccaatctcaa gctcctgcca tgaactttct
     2881 caaagtctac agatttttta gctcatccta aagcaacggt tctgtatagt cgaattaacg
     2941 aatatattaa aagctactgc taaggtatgg caggactcgg cttggcctgt cctagttcga
     3001 tgactttacc gcaaaatcaa cggggtgtag ttatggcaat atgtctcgta ctgttttgct
     3061 aatcgatagt gtgtaatgcg gtgtactcag caatgcaatc tgaaaattct ttgttaagtg
     3121 tgctggtgat tgctttaaat tcgtgctcgg tttggctgtg tatattaagc acaatagtaa
     3181 aagtgcctag gcaccgggat tggcgtacga tattcgggtg tgcccggcaa gtctcccaaa
     3241 aagcattgat cctagcaggt ttggggctga gtgtataaaa gcacagtgta tttgtatagc
     3301 ccagcaactc gtgattgatt attggcacac attttaaaag gacgcccgtt gccagtaatg
     3361 atcggtagtg cgactgtact gtgtcatagg ttttttctag gcgttttgcc atatcaagca
     3421 acgatagggt ggggcgcttg gtcaataggt tcactatgtc tcgctgcagg ttagtgagct
     3481 ctattttttt agttgaatgt gtgtaggaca cattttctgc atggggttcg ctatacaaat
     3541 agttatggtc ggctacagaa ccttggtagt ccat
//
LOCUS       DATNGP010000067         3184 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_1889530, whole genome shotgun sequence.
ACCESSION   DATNGP010000067 DATNGP010000000
VERSION     DATNGP010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3184
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1889530"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..782
                     /locus_tag="VN778_01635"
     CDS             <1..782
                     /locus_tag="VN778_01635"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HXR49709.1"
                     /translation="SLSELGDILNSLRTVSEDASVAGLIDSLLRRIDYLHFLDDGTPQ
                     GEARQENVRELLSVAQEYQDAGLDGFLEEVALVSDLDGADFTGDAVTLMTLHAAKGLE
                     FPVVFMAGMEESIFPHSRALYDLHEMEEERRLCYVGMTRARQELYMLYASSRLLYGGV
                     QHNPPSRFLSEIDGDFQASTLSAQFTADPLQSLAYSEPAAPASSNEIRYVPELNEGDS
                     VRHQIFGTGTVMEIEGDTAVVYFKGKGARKLNISFAPLEKL"
     gene            940..2142
                     /locus_tag="VN778_01640"
     CDS             940..2142
                     /locus_tag="VN778_01640"
                     /inference="COORDINATES: protein motif:HMM:NF019142.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HXR49710.1"
                     /translation="MSRHPFAVPVITVWALIILSLGGYLVARQTNHLPPVQDAKIVII
                     SHDHEQQIVPSREPTVGTLLAKLHLALHEGDVVEPAASTPIDQNEFRINIYRAVPVEI
                     VDGTQKTFAFSAGTTPRAIAQQTGARVYSEDDVTTDPTQDFVSQGTIGEQVVIDRSKP
                     VLLNLYGTPLAIRTHAPTVGDLIRQKHIHLAKDDQVTPDQNTPLTANSQVEIARNGTK
                     LETVTSPIAMPIQTINDPSLAYGTNAVRQQGTPGQQVTTYQDTLRNGVVVGRAAVQTI
                     VTQQPVTQIVVVGTSLSGIKGDMALAGIAPGDYQYADYIISRESGWCPTKAQGQYGGC
                     PPYSGYVPSYGGYGLCQSTPGIKMSSAGADWATNPITQLRWCSGYASAHYGGWYNAYI
                     HWVNNHNW"
     gene            2148..2936
                     /gene="rsmA"
                     /locus_tag="VN778_01645"
     CDS             2148..2936
                     /gene="rsmA"
                     /locus_tag="VN778_01645"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HXR49711.1"
                     /translation="MNEPLAKKSLGQHWLYDVASLHAMCEAADIGREDVVLEIGPGLG
                     TLTEVLMSQAAQVIAVEFDEKLAAELPHRVPPDNLHVIQQDILSFDFTSLPASYKVVA
                     NIPYYLTSNLIRVMSETPNPPQVAALLVQKEVAERVAAEPGSMSLLSVTAQYYWEVSL
                     GQVVPAELFTPPPKVNSQILILTLRPQPLFPDVDSKEFFRLVKAGFAQRRKTLLNSLS
                     TGLHLSREAVQSWCQKAGIDPQRRPQTLSLDDWYSLYKFGSRSE"
     gene            2983..>3184
                     /locus_tag="VN778_01650"
     CDS             2983..>3184
                     /locus_tag="VN778_01650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HXR49712.1"
                     /translation="MGPTNNLSYLIQHLAAVMGKQSDQLLQEQLGIGYSQYKILMVLE
                     WNPRVQQRTIAESLGQTEASISR"
BASE COUNT          760 a          866 c          862 g          696 t
ORIGIN      
        1 aaagcctgag cgagttaggc gacatattaa acagcttgcg cacggttagc gaagacgcta
       61 gcgtagccgg actgattgat tcgctacttc gccgtatcga ctacttacat tttttggatg
      121 acgggacacc gcagggtgaa gcacgccagg aaaacgtccg cgagctgttg agcgtggcgc
      181 aagagtacca agatgccggg ttggacggct ttttggagga agtcgctctg gtcagtgatc
      241 tggatggtgc cgattttacc ggtgatgccg tcacgctgat gacgctgcat gctgccaagg
      301 gcttggaatt cccagtcgtc tttatggccg gtatggagga gtcgattttc ccgcattccc
      361 gggcgctcta tgacctgcac gaaatggaag aggagcggcg gctttgttat gtgggtatga
      421 cgcgggcgcg ccaggagttg tacatgctct atgctagcag ccgtctgctg tatggcggcg
      481 tgcagcataa tccgccgagc cgcttcctca gcgaaatcga tggtgatttc caggctagca
      541 ctttgagcgc tcaattcacg gccgaccccc ttcagtccct agcgtatagt gaaccggcgg
      601 cacctgcctc ctcaaatgag atccgctacg tgcctgaact taacgaaggc gatagtgtcc
      661 gacatcagat cttcggtacc ggtaccgtca tggaaatcga aggtgacacc gcggtagttt
      721 attttaaagg caagggcgcc cgcaagctga acatcagttt tgcgccgctt gagaagttat
      781 aagtcagggt agcgagctgc tggctagccg ctacacaggt gtttttgcta caatgaagcg
      841 gtatttgaga tgatttctga caagcttgaa aagttacgga taaaatttgt gcgttatcgc
      901 cgcgtacggc taccgcgccg gattcgtaaa ctcaaaatca tgagccggca cccgttcgcg
      961 gtaccagtga tcacggtctg ggcgctgata attctcagtc tggggggtta tctagtggcg
     1021 cgccaaacta accatttgcc gccggtacaa gatgccaaaa ttgtcattat ttcgcatgat
     1081 catgagcagc aaatcgtacc gagccgcgag ccgaccgttg ggacactact agctaagctt
     1141 catttggcat tacatgaggg cgatgtcgtc gagccggcag ctagtacgcc gatcgaccag
     1201 aacgagttcc gcatcaatat ttaccgggca gtgccggtcg aaatcgttga cggcacccaa
     1261 aagacctttg cttttagtgc cgggaccacg ccgcgggcta tcgcccaaca gacgggcgcc
     1321 cgagtgtact ctgaagacga tgttacaacc gatccaaccc aggacttcgt aagccaggga
     1381 accatcggcg aacaagtagt tatcgataga tccaagccgg tactgctcaa cctctacggc
     1441 acaccgctgg ccatccggac ccatgctccc actgtcggcg atttgatcag gcaaaagcac
     1501 atccacctgg ctaaagatga ccaggtcaca cccgatcaaa atactccgtt aactgccaat
     1561 tcgcaggtgg agattgctcg taatggcacg aagctcgaaa cggttaccag cccaatcgcc
     1621 atgccgattc agacgatcaa cgacccgagc ctggcgtacg gcaccaatgc tgtccgtcag
     1681 caaggcacgc ccgggcaaca ggtcacaacg tatcaggata cgctgcgtaa tggcgtcgtc
     1741 gtcggccggg ccgcggttca gacaattgtc acgcagcaac cggtgacgca aatcgttgtg
     1801 gttggtacat ctctcagtgg catcaagggt gacatggccc tcgccggtat tgcccccggc
     1861 gactaccagt atgccgacta cattatcagc cgcgaatcag gctggtgtcc gacgaaagcc
     1921 caaggccagt acggtggctg tccgccttac agcggctatg taccctcata tggtggctat
     1981 ggtctctgtc agtctacacc cggcatcaag atgtcgtcgg ccggcgccga ctgggccacc
     2041 aaccccatca cccagcttcg ttggtgctcc ggctacgcct ccgcccacta cggcggctgg
     2101 tacaacgcct atatccactg ggttaacaat cacaactggt aaatggcatg aatgagccgc
     2161 tggctaaaaa gtcgttaggg cagcactggc tgtatgatgt ggcgagcttg cacgccatgt
     2221 gtgaagctgc cgacataggc cgggaggacg tagtgctgga gatcgggccc gggctcggca
     2281 cgctcaccga agtactcatg agtcaggcgg cacaggtcat tgctgtggag tttgatgaaa
     2341 agctggcggc cgagctaccg catcgagtgc cgcccgacaa tctgcacgtt atccaacaag
     2401 atattttgag ctttgatttc acgagcttgc cagcgagtta caaagtggta gctaatatcc
     2461 catactacct aacgagcaat ctaatccgag tgatgagcga aacacctaac ccaccccagg
     2521 ttgctgcgct tctagtccaa aaagaagtgg ccgaacgagt tgccgcagag ccgggaagca
     2581 tgtcgttgct cagtgtaact gcacagtact attgggaagt aagtctcggg caagttgtac
     2641 ctgcagagct attcacgcca ccgccgaaag tcaactcgca aatcctaatt cttacgcttc
     2701 ggccccaacc actttttcct gacgtcgata gcaaggagtt ctttcgtctt gtcaaagctg
     2761 gctttgccca gcgacgtaaa accctcctta atagtttgag tactggcttg catctgagtc
     2821 gcgaagcagt gcaaagctgg tgccaaaaag cgggcattga cccgcagcgc agacctcaga
     2881 cactgagtct cgatgattgg tactcactct ataaatttgg ctcccgttcc gagtaaaaaa
     2941 tattacttga tgtgttaagt attgacagtt agactgttaa gtatgggacc gacgaacaat
     3001 ctcagctacc tgatacaaca cctggctgcc gtcatgggca agcaatcgga ccagctctta
     3061 caggagcagt tgggcattgg ctatagccag tataaaatcc ttatggtgct ggaatggaat
     3121 cctagggtac agcagcgaac cattgccgaa agcctcggcc aaaccgaagc cagcatcagc
     3181 cgcc
//
LOCUS       DATNGP010000068         7357 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_1873789, whole genome shotgun sequence.
ACCESSION   DATNGP010000068 DATNGP010000000
VERSION     DATNGP010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 7357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7357
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1873789"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            116..799
                     /locus_tag="VN778_01655"
     CDS             116..799
                     /locus_tag="VN778_01655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49713.1"
                     /translation="MYKAGKRPKSKRWRALKWVLLTVVLLACIGWAGQAIRHALKPKT
                     VITQGRAVTTHVSYTTKTKHYNEGDFALDIPDTWQPIPRPAGPYKSFTWQSSDRITDG
                     QVIEIYEDTIPENFSVNRVLIVEGENDHLMINGSISDNCSNYTDQFSSANPLAAPAKW
                     QGVAFLCDQANQERDVIGTSSTDGINTVILKSQSAGASHKFLFTYTDYSVNPDYTVFL
                     NVLNSLRMN"
     regulatory      960..1089
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            1145..2359
                     /locus_tag="VN778_01660"
     CDS             1145..2359
                     /locus_tag="VN778_01660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49714.1"
                     /translation="MEALVEQPDTAEKQKSFDPLASIHLLRYDLDHYGEVLPETRQRV
                     CDEELSYLVEGIDKAACTSFLIKRDGDDLVYFSQSTWRPYMGALLTGLGIARNEAEKD
                     PRRQFLADRAVDDLRHGYELLAMQPGEQKVWHSPYAHSEEAQYGEEFIKDCGMNPQRK
                     MGFLYQAVCQHDGSILLKSQTVDRSDDDAFQAVNQAAAADPQINMEAMVDIYDEHLQE
                     KFGDHFFAGRRESKKNENAWDEIHNHRDLIEFHLNKLEAIASQAIAGAELENVTKRHL
                     YGVWASFKKRLSGESISHTSVNYSYASHLYDPELHHMLEVEVRQNFKEFVSRGEVLVG
                     CGGAIRIVNSENDVMDADASDVHTVIFGGSDSDRYGSLTFKCPAGHHNRRPRGVLLTK
                     CQTSNCKAKVTC"
     gene            2410..3000
                     /locus_tag="VN778_01665"
     CDS             2410..3000
                     /locus_tag="VN778_01665"
                     /inference="COORDINATES: protein motif:HMM:NF013621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49715.1"
                     /translation="MARVGIYAGAFNPVHAGHIGFALQAVEQAGLDRLYFLPERRPRF
                     KQGVEHFGHRVAMLRRAIKPHPKFSILELPDISFSVERTLPRLQQALPGQELVFLFGS
                     DVAARLAGWPDVEHLLGTSELIIGVHGLDTEALVKQQLESLSKQPQALHLFVSHAPKV
                     SSRQVRNALRRREYVQGLLKSVQRYSDKNWLYVSLA"
     gene            3083..3781
                     /locus_tag="VN778_01670"
     CDS             3083..3781
                     /locus_tag="VN778_01670"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HXR49716.1"
                     /translation="MSKQFLGIIVVVILVFVGIFLFTGNKSSGGNKSSGGNSSSLTQH
                     VEGQGKDGVTLVEYGDYQCPFCGQYYPIVKQVQAEYNEQIHFQFRNFPLVSIHQNAFA
                     GARAAEAAAQQGKFWQMHDLLYDAQSQWSESGDPTSFFNQYAQQLGLNITQFKSDYAS
                     DKVNNLINADMGQGNKLGITGTPSFFLDGKQIQVGESVQAFEKVINAEIAKKAPAASS
                     TSSSSASSTSSTTT"
     gene            3878..4927
                     /locus_tag="VN778_01675"
     CDS             3878..4927
                     /locus_tag="VN778_01675"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HXR49717.1"
                     /translation="MTNYILAAILLILALGGISLRKTYFYLPVRELKRRAEHHDTVAA
                     KFYSAVAYGNSLRSLLWLYIGLTSAGSIILLARQLPVWASLLIVAPLLWAAFSLLPAT
                     RLTKPSTQLTLFITPAIARLLNYLHPALNRGADIVEKRYTRSMHTQLFEREDFMELIE
                     RQQAQEDSRLSGEELEIIKRALSFSDRKVIDILTPRKAIKIVLADDTIGPILITELHE
                     NKQSYALVRETAKGAFVGTLSVRQLGLRSSGSARQVMDSAVYYLHEEDSLSQALHAFF
                     ATNKSVFVVVNSFEEFTGIVTIESILKELLGHIPGDDFDQYANPAAVAARHTKPKKSE
                     KPGDEPVKTENEVVE"
     gene            4937..6703
                     /gene="aspS"
                     /locus_tag="VN778_01680"
     CDS             4937..6703
                     /gene="aspS"
                     /locus_tag="VN778_01680"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HXR49718.1"
                     /translation="MARTLVKDTVGQIGQIVTLKGWVHSRRDHGGLIFIDIRDHTGVA
                     QLVVQPENADTFATAEHVRDEYVIEAQGLVRERVADLKNPNIATGDIELKVDELKVLN
                     SSEALPIQIHGETLANEDLRLKYRFLDLRRPKMQETLKKRAEYYKIMREYMDQKDFID
                     VSTPILANSSPEGARDFLIPSRVHPGKFYALPQAPQQFKQLLMVGGVPRYYQLAACFR
                     DEDPRADRLYGEFYQLDLEMSFVEDGEEVRSLMDPLIKKLVTEFAGKQLMDEKIPRIP
                     YQEAMERYGSDKPDLRFGMELIELSDELKDTEFGVFASTLKDGGCVKAICAPGAAALS
                     RSQIDQFTEIAKAEGAGGLAYLTFENGEIKSPIAKFLKPEELNAIKQRAGAKDGDIVF
                     FGADKRALVNKVLGRLRNELADHFDMKDPNIIALAWIVDFPFYEWDDKNKRLDFGHNP
                     FSMPKGGLEALEAEDKYSILADQYDMVMNGYEVCSGAVRNHHPQVMYKVFAALGYDKD
                     YVDARFGAMINAFKYGAPPHAGCAFGIDRMFMVLIGEDNIREVVAFPKNGSGVDTMVS
                     SPSPVDAAQLKELNVDVKPTTE"
     gene            6723..>7357
                     /locus_tag="VN778_01685"
     CDS             6723..>7357
                     /locus_tag="VN778_01685"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HXR49719.1"
                     /translation="MDIPVKKLKSGFTLPVYGLGTYLMGGRTEPDTANDQAEIAAIKG
                     ALDRGVTHIDTAEVYGGRGHTEELVGEAIKGYDRANIFITSKLRGFTTGTREDIFTSC
                     QASLKRLGTDYLDLYLLHKYVDPGVSIEGVMSAMNDLIKEGLVRNIGVCNMTVNRFKE
                     VQKYSEAKLVCNQVHYSVQMREAEARGLTKDAIENDYFITAWGPLEKGLLG"
BASE COUNT         1909 a         1903 c         1887 g         1658 t
ORIGIN      
        1 ctatacgtcc tgatcggcgc ccttaaagtc cttggtctcg acggtgccgc ccaaagcttt
       61 atgccgcgcc tcagcgttct caaacaaaag caacctactg cctaaggaaa tctacgtgta
      121 caaagccggc aaacgtccaa agagtaagcg gtggcgagcc ctgaagtggg tgctgctcac
      181 tgtagtcctg cttgcttgta tcggctgggc cggtcaggca atccggcatg ccctaaagcc
      241 gaaaacggtc atcacacagg gcagagcggt tacaacgcat gtttcgtata ccaccaagac
      301 gaaacactat aacgaaggcg actttgcgct tgatatacca gacacttggc agccgatacc
      361 gcgccccgcc ggcccttaca aatctttcac ttggcagagc agcgaccgca ttacggacgg
      421 gcaagtcata gaaatttacg aagacactat tcccgagaat ttttcagtca accgcgtctt
      481 gatcgtcgag ggtgagaatg accacctgat gattaatggc tcgatctcgg ataactgttc
      541 taactacacc gatcaattca gctctgccaa cccgctcgca gctcccgcca aatggcaagg
      601 cgtggctttc ctctgcgacc aggctaacca ggagcgcgac gtcatcggta ccagctcgac
      661 cgacggcatc aatacggtga tcctgaagag ccagtcggcc ggcgccagcc acaagttcct
      721 cttcacctac accgactact cagtcaaccc tgattacaca gtcttcctca acgtactcaa
      781 tagcttgcgc atgaactagc tacctatttt catagttatc ggaagtagtg tccacatagt
      841 atggggacac aatgtggaca ctgccgctgg gatacaataa aaattctacg ccatagagca
      901 caagctaccc tatctcacac tatttatggt aattaatact atatctagcg tgccggcgag
      961 ctgctacact actacatagc tttaacaaaa catgctggaa gtgggtgcga atcccacact
     1021 gtgccgcaac tgtgaatccc gcctctcggg cgtggaaagt cagatacagc tttaaaagtc
     1081 tctcgagcaa gagcaaaatt gacccccgtc tctttgccct cgcttaagag ccgagggtag
     1141 actcatggaa gcattggtcg agcagccaga tacggcagaa aaacagaaaa gctttgaccc
     1201 cctagcaagc atccacctgt tacggtacga ccttgatcat tatggcgagg tcttgccgga
     1261 aactcgacaa agagtttgtg atgaagaatt gagctacttg gtggaaggga tcgacaaagc
     1321 cgcctgcacc tcgtttctta ttaagcgcga tggtgatgat ttagtctact tctctcaaag
     1381 tacctggcgc ccctatatgg gcgctttgtt aacgggttta ggaattgcac gcaacgaagc
     1441 cgaaaaagac cccaggcgtc aattcttggc agatcgtgct gttgatgatt tacgacacgg
     1501 ctacgagctg cttgctatgc agccgggaga acaaaaggtt tggcattcac cctatgctca
     1561 tagcgaagag gcacaatatg gcgaggagtt tataaaggat tgcgggatga atccgcagcg
     1621 caaaatggga ttcctgtacc aggcagtttg tcagcacgac ggcagcattt tgctcaaatc
     1681 gcagacagtt gatcgaagtg acgatgacgc ctttcaggcg gttaatcaag cggcagcagc
     1741 tgatcctcaa attaatatgg aggccatggt tgatatatac gatgaacatt tacaggaaaa
     1801 atttggtgac cacttctttg ctggccgccg ggagtcgaag aaaaatgaaa atgcctggga
     1861 tgaaatccat aaccatcgcg acctcatcga atttcaccta aataagcttg aggcaatagc
     1921 ttctcaagct atagcaggag cagagcttga aaatgttaca aaaagacacc tctacggcgt
     1981 atgggcatca tttaaaaagc gtttaagcgg cgagtcaatt tctcatacct cagtcaacta
     2041 ttcgtatgcc agccatctat atgatcccga actgcatcac atgctcgaag ttgaggtgag
     2101 gcaaaacttt aaggagtttg tctcgcgcgg tgaagtcttg gtgggatgcg ggggagcaat
     2161 tcgtatcgtt aatagcgaaa acgatgtcat ggacgccgat gcttccgacg tacacactgt
     2221 aatattcggc ggctctgaca gcgacaggta cggttcgctt actttcaagt gcccggcggg
     2281 gcatcataac aggcgaccac gtggtgtgct gttgacgaaa tgccagacgt cgaattgtaa
     2341 ggccaaggtc acctgctaaa gcgcgccctg ggcgtataat tatacgtatg gcaagtaatc
     2401 aaaacgtaga tggctagggt cggcatttat gcgggggcat ttaacccggt acacgccggg
     2461 catatcggct ttgctttgca ggcggtcgag caggctggcc tggatcggct gtatttcctg
     2521 ccggagcgcc ggccgcggtt taagcagggc gtggagcatt tcggacatcg ggtggctatg
     2581 ctcaggcggg cgatcaagcc gcatcccaaa ttcagcatct tagaattacc cgacattagc
     2641 tttagtgtag agcggacttt gccgcgtttg cagcaggcct tacccggtca ggagttggtg
     2701 tttttgtttg gttcagacgt ggctgctcgg ctggctggtt ggcctgatgt tgagcacttg
     2761 ctgggcacga gtgagttgat aatcggtgtt cacggcctgg ataccgaagc cctcgtcaag
     2821 cagcaactag aatctctgtc gaaacaaccg caagcccttc atttgttcgt cagtcacgcg
     2881 ccaaaggtat cgtcgcgcca ggtgcggaat gccctgagac gacgcgaata cgtgcaaggc
     2941 ttgctcaaaa gcgtacaacg ctatagcgac aaaaactggc tatacgtgtc gctggcttaa
     3001 agctaactga agagtaatta agaaatagaa aacacgtatt gtatactaga aataagttaa
     3061 tctttggggg agccagacag atatgtcgaa gcaatttttg ggaataatcg tggtcgttat
     3121 cttggtgttt gtcggcattt ttctctttac cggcaataaa tcttcgggcg gcaacaaatc
     3181 ttccggtggt aacagcagca gcttgacaca gcacgttgag gggcagggca aagacggcgt
     3241 gacacttgtt gagtatggtg attatcagtg cccattttgc ggacaatatt acccgatcgt
     3301 caaacaggtc caggctgaat ataacgagca aattcacttc cagttccgca acttccccct
     3361 ggtgagcatt caccaaaacg ccttcgccgg ggcccgggcc gccgaagctg ctgcccagca
     3421 aggcaaattc tggcaaatgc acgacctgct gtacgatgcg cagagccagt ggtcggaatc
     3481 cggtgatccg acatcgttct ttaatcagta cgctcagcag ttggggctaa atattacgca
     3541 gttcaagtcg gactacgcta gcgacaaggt caacaacctc attaatgccg atatgggcca
     3601 gggcaacaag ctcggcatca ccggcacgcc aagcttcttc ctcgacggca agcagatcca
     3661 agtcggcgaa agcgtccaag ctttcgaaaa agtgatcaat gccgagatcg ccaagaaagc
     3721 tcccgccgcc tcctcaacaa gcagctcctc agctagttcg acctcttcta ctacaactta
     3781 gtagtgcaaa aattcgcgcc actggggacg ctcgacctgc accggcacga gagacagctc
     3841 ttttcaagca tccaaagtcc atataagcta aatatgcatg acaaattaca ttctggcagc
     3901 tatcttgctt atcttggcac tcggtggcat cagcttacgc aaaacttatt tttacctgcc
     3961 ggtgcgcgag ctgaaacggc gcgctgaaca tcacgacact gtcgccgcta agttttattc
     4021 cgcggtggca tatggcaata gcttgcgcag cttgttatgg ctctatatcg ggctaaccag
     4081 tgccggaagt attatcttgc tcgcccggca actccctgtc tgggcaagtc tgctaattgt
     4141 cgcgccgctg ctatgggcag ccttttccct cttaccggcc acccgcttga cgaagccgag
     4201 cacccaatta acgctgttta ttactccggc aattgccagg ctcttgaact accttcatcc
     4261 tgcactgaat cgtggcgcgg acatagttga gaagcgctat acgcggtcga tgcatactca
     4321 gctgtttgag cgtgaagact tcatggagct aatcgaacga cagcaagcgc aagaagatag
     4381 ccggctgagc ggggaagagc tggagatcat caagcgagcc ctcagcttca gtgaccgcaa
     4441 agtgatcgac atattgacgc cgcgaaaagc catcaaaatc gtgctggcgg acgatacgat
     4501 cggcccgata cttatcaccg aattgcacga aaacaagcag agctacgcgc tggttcgtga
     4561 aaccgccaag ggtgcttttg tcggcacact atctgtgcgc cagttgggat tgcggagtag
     4621 tggcagtgcc cgccaagtca tggacagtgc tgtctactat cttcacgaag aagatagtct
     4681 cagccaagcc ttacacgcct ttttcgccac caataaatca gtttttgtag tagtaaacag
     4741 ttttgaagaa tttaccggta tcgtcaccat cgaaagcatc ctaaaagaac tcctgggtca
     4801 tatccctggc gacgacttcg atcaatacgc caacccggca gccgtcgccg cccgccatac
     4861 gaaacccaaa aagtccgaaa aacccggcga tgaacctgtt aaaacagaga acgaagtggt
     4921 agaataaccc cgatacatgg cgagaacatt agtcaaagat acagtcggac aaataggcca
     4981 aatagttacc ctgaagggtt gggtgcattc gcggcgcgat catggtgggc tgatttttat
     5041 tgacatccgt gaccacactg gggtggccca actggttgtg cagccggaga atgctgatac
     5101 ctttgcgacg gctgagcatg tgcgcgacga atacgttatt gaggcgcaag gcttggtgcg
     5161 ggagcgtgtc gccgacctta aaaatccgaa cattgcgact ggcgacatcg agctaaaagt
     5221 cgatgaactg aaggtcttaa acagctcgga agcgttgcct atccagattc atggcgagac
     5281 gctagccaat gaagacctac ggcttaagta ccgttttctc gatctgcgcc ggccaaaaat
     5341 gcaggaaacc ctgaaaaagc gcgctgaata ttacaaaatc atgcgcgagt atatggatca
     5401 gaaagatttc atcgacgttt cgacgccgat tctcgcgaat tcgagccctg aaggcgcacg
     5461 cgatttcctc attccctcgc gtgttcaccc cggcaagttc tatgctttgc cacaggcacc
     5521 acagcagttc aagcagttgc tgatggtggg cggcgtgcca cgctattatc agctggcggc
     5581 ttgctttcgc gatgaagacc cacgagccga ccgcttgtac ggcgaatttt accagctcga
     5641 tctggaaatg tcgttcgttg aagacggcga agaagtgcgt tcgctcatgg accccttgat
     5701 caaaaaacta gtcacggagt tcgccggcaa gcagctgatg gacgaaaaga taccgcgtat
     5761 tccctaccaa gaagccatgg agcgatatgg ttcagacaag cctgacttgc gatttggcat
     5821 ggagctgatc gaactatcgg atgaactcaa ggataccgaa ttcggcgttt ttgctagcac
     5881 acttaaggat ggcggctgcg tgaaagccat ctgcgcacca ggcgccgccg cactcagtcg
     5941 ttcccaaatc gatcagttca cggaaatcgc taaagctgaa ggtgccggcg gcctcgccta
     6001 cctcactttt gaaaatggtg aaatcaaatc cccgatcgct aaattcttga agcccgaaga
     6061 gctaaacgcc atcaagcaaa gggctggtgc taaggatggc gatatcgttt tcttcggcgc
     6121 cgacaagcga gcgctagtca ataaagtgct tggccgcttg cgtaatgagt tggccgacca
     6181 cttcgatatg aaggatccga acatcatagc gctggcctgg atcgtcgact ttccatttta
     6241 cgaatgggac gacaaaaata agcgcctgga ttttggccac aatccgtttt cgatgcccaa
     6301 aggcggcctc gaagccctcg aagcagaaga caaatacagc atcctcgccg accaatacga
     6361 catggtcatg aatggctacg aagtttgctc cggcgctgtc cgaaatcatc atccacaggt
     6421 catgtacaaa gtctttgcgg ctctcggcta cgacaaagac tacgtcgatg cccgtttcgg
     6481 cgccatgatc aacgccttca aatacggcgc gccgccacac gccggctgtg ctttcggcat
     6541 cgaccgcatg tttatggtgc tgattggcga ggacaacatc cgagaagtgg tcgccttccc
     6601 caaaaacggc tccggtgtcg acaccatggt gagctcgccc agccccgttg atgcagccca
     6661 gctcaaagag ctcaatgtcg acgtcaagcc caccaccgag tagtgcctgc tacaattgat
     6721 acatggacat tcctgtcaaa aaattaaagt cgggctttac gttaccggtg tacggtctgg
     6781 gcacatattt gatgggcggc cgtacagagc ctgacacggc aaacgatcag gctgaaattg
     6841 ccgctatcaa aggagcgctg gaccgaggcg tgacccacat cgatacggct gaagtttacg
     6901 gcggcagagg gcataccgag gagctggtag gcgaggccat caagggttac gaccgggcaa
     6961 atatatttat aacttctaag ctacgcggct ttaccactgg cacccgtgaa gatatattta
     7021 cttcctgtca ggccagtctc aaacggctgg gcacagacta cctcgacctc taccttttgc
     7081 acaaatatgt tgacccgggc gtgtcaatcg aaggcgtaat gtcggctatg aacgatctta
     7141 tcaaggaagg tttggtcaga aacattggcg tttgtaatat gacggttaat cgatttaaag
     7201 aagttcagaa atattcagaa gccaagctgg tatgcaacca agtccactac agcgtgcaaa
     7261 tgcgcgaagc cgaagctcgc ggactcacga aggatgccat tgaaaatgat tactttatta
     7321 cggcctgggg gccgctcgaa aaggggcttt tgggtca
//
LOCUS       DATNGP010000069         3788 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_4623272, whole genome shotgun sequence.
ACCESSION   DATNGP010000069 DATNGP010000000
VERSION     DATNGP010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3788)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3788)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3788
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4623272"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..891
                     /locus_tag="VN778_01690"
     CDS             <1..891
                     /locus_tag="VN778_01690"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HXR49720.1"
                     /translation="QSTEGTRPPRIMSNLEISRAQRLDQKVIEVLPELSRGFAARLVE
                     DGKVTVNGVAQTKPGYKLREGDAVVVDYDTAELAKIPDIELEVLYEDEDCVVINKPIG
                     VLSHSKGAFNPEATVATWLRSRLNGLSGERGGIVHRLDRATSGVMICAKTPEALSWLQ
                     KQFATRKVKKTYAAVVAGELSLPEAVVDMPIGRNPKAPATFRVDVQGKYAKTAYKTLR
                     TNGRYSLLELKPETGRTHQLRVHLRHLQHPIVGDPLYGGEVADRLYLHAQQLEITILN
                     GHERKVFTAPLPAAFTQYVP"
     gene            888..1757
                     /locus_tag="VN778_01695"
     CDS             888..1757
                     /locus_tag="VN778_01695"
                     /inference="COORDINATES: protein motif:HMM:NF024575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXR49721.1"
                     /translation="MNVPGIVLHVASQTQLDALVVTPAHAVLLSGPVGIGKTHIAVAL
                     GAQLLKVSPETLLHHAYYQRITPTKGTITIEQVRELISFFKLKVPGKAPIKRLAVVED
                     AEAMGTEAQNALLKLLEEPPTDSVLLLTSSRPDRLLSTIRSRLRQLQLPAPAEAALLE
                     HFSAAGYDRVRIQTVLLRAGTSGAEVKRILEASDNESTETVALVKQVLGMSQYERLLL
                     VDSLAKQKEQTRLFTETLAMMATASLEAAATKNAPTTSRWRDILQAAYTAQNALEQSG
                     NAKLVLTELMLAI"
     gene            1784..2437
                     /locus_tag="VN778_01700"
     CDS             1784..2437
                     /locus_tag="VN778_01700"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXR49722.1"
                     /translation="MYELVIVAMFGLLGIASTRLHAEEFNEVSHKVGDRMGKLWDIAH
                     QGMRENRFLRAEKALLTILKIDEKNAAAYNRLGILYAKQKEYRDAIDCFEIASSIEAT
                     PSSLHNLGLIYYETENYEKAAIAFESALKLEESLAARHVAYAKVHEKLGNEKLMFQAL
                     HRAIELEPNKETYTLLLQAYQERGMDAEADMIAEKLKKLIVPAGRPRRILRPRRVVI"
     gene            2485..2560
                     /locus_tag="VN778_01705"
     tRNA            2485..2560
                     /locus_tag="VN778_01705"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2518..2520,aa:Thr,seq:tgt)
     gene            2679..2763
                     /locus_tag="VN778_01710"
     tRNA            2679..2763
                     /locus_tag="VN778_01710"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2713..2715,aa:Tyr,seq:gta)
     gene            2855..>3788
                     /locus_tag="VN778_01715"
     CDS             2855..>3788
                     /locus_tag="VN778_01715"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HXR49723.1"
                     /translation="MSSTEAMLAIEPILDTVPQYEDFLTFAEIRSRSAVLADTYPDSL
                     RIETIGESAEGRPLELLVVGNTQSLDTFGPEQGRRTALIFGAPHPNEPSGVHTSFVMA
                     EYFAKYPDRLQDFGFDTLLVLPAADVDGLVLNEDGFKGFSPEKYLENFYRPPFKDQPE
                     WSFPINIPELGLFFDQPMPVAAALATVMDAWRPRFVGSLHNSWTETGAYFTANRHIGK
                     LAGIVQEFSERYGIPLHQGPNEQPYGQELHPGVYTHASRIVETVWNKIHGKANTKEAG
                     GSYDYLLRHALAGKNYQAMIEAGTLNLDGEAEDHP"
BASE COUNT          970 a          986 c          984 g          848 t
ORIGIN      
        1 caaagtacag aaggcactag gcctccgcgc ataatgagca atctggaaat tagccgagcc
       61 cagcgccttg accagaaggt cattgaggta ctgccagaac tcagccgcgg ctttgctgcg
      121 cgcctagtcg aagacggcaa ggtaaccgtt aacggcgtcg cccagaccaa gccgggttac
      181 aagcttcgcg aaggtgacgc agttgtcgta gattatgaca ccgcagagtt agctaaaata
      241 ccggacatcg agctggaagt actatacgaa gacgaggact gcgtggttat taataagcca
      301 attggggtac ttagccacag caagggcgca ttcaaccccg aggccacggt agctacctgg
      361 ctacgttcgc gccttaatgg gcttagcggc gagcgaggtg gaattgtgca tcggctagat
      421 cgcgccacta gtggggtgat gatctgcgcc aagacgccgg aggcgctcag ctggctgcaa
      481 aagcaattcg ctacccgaaa agttaagaag acttatgctg cagtggtggc cggtgaactt
      541 tctctgcctg aagcagttgt tgatatgccg atcggccgta atcccaaagc accagcaacc
      601 ttccgggttg atgttcaggg caagtatgct aaaaccgcat ataaaacgtt gcggacgaac
      661 gggcgttata gcctgcttga gctaaagccc gaaacgggcc gcactcatca gcttcgggta
      721 catttgcgcc atttgcaaca tcctatcgtt ggtgacccac tgtacggtgg agaagtcgcc
      781 gataggctct acctccatgc ccagcagcta gagatcacta tcctaaacgg ccacgaacgc
      841 aaagtcttta ccgcgccact gcccgctgcg tttacccagt atgtgccatg aacgttccag
      901 gcattgtact ccacgtagcc agccaaactc agcttgatgc gctggtagtc acacctgcgc
      961 atgccgtgct actaagcggc cctgtgggaa tcggcaaaac acacatcgcc gtagcacttg
     1021 gtgcgcagct cctaaaagtt agccccgaga cgcttttgca ccatgcgtat taccagcgaa
     1081 tcacgcctac aaaaggcact attaccattg aacaagttcg agagttgatt agttttttta
     1141 agctaaaagt cccaggcaag gcgcccataa aacggcttgc cgtcgtagaa gacgccgaag
     1201 caatgggtac cgaggcacag aatgcgctac taaagctact cgaggaacca ccaacagact
     1261 ctgttttgct tttaactagc tctcggccgg atcgtttgtt gtcaacaatc cgctctcggc
     1321 tgcggcagct gcaattgcca gcacctgctg aagctgcact attagagcac ttcagtgctg
     1381 ctggctacga cagagtacgc atccaaacgg tgctactacg agccggtacc agcggtgccg
     1441 aagtgaagcg catcctagaa gccagtgata atgaatccac tgaaacggtt gccctagtta
     1501 agcaggtact tggtatgtct cagtacgaac ggcttctact agtagactca ctcgccaagc
     1561 aaaaagagca gacccgtctc tttactgaaa cattggccat gatggctacg gctagcctag
     1621 aggcggccgc aactaagaat gcgcccacga cctctcggtg gcgagacatt ctgcaggctg
     1681 cctatacggc acaaaacgct ctcgagcaaa gtggtaacgc caaactagta ctgactgagc
     1741 ttatgcttgc catatagatt tgtgatatac taacaagcat tgtatgtacg agttagtgat
     1801 cgtcgctatg tttgggcttc ttggtattgc cagtacccga ctacatgcgg aggaatttaa
     1861 cgaggtttcc cataaagtcg gcgaccgtat gggtaagctg tgggatattg cgcatcaggg
     1921 tatgcgtgag aaccgtttcc taagagccga aaaggccctg ctaaccatct taaagattga
     1981 tgaaaaaaat gccgctgcgt ataaccgcct cggcattctt tatgccaagc aaaaagaata
     2041 tcgcgacgcg attgattgct ttgagattgc cagcagtatc gaagctacgc cgtcgtcgct
     2101 ccacaaccta gggcttatct actacgaaac cgagaactac gaaaaagccg ccattgcctt
     2161 cgagtctgct ttgaagttag aggaatcact ggctgctcgc catgttgctt acgccaaagt
     2221 acacgaaaaa ctaggcaacg aaaagctcat gtttcaggcc ttgcaccgcg ccatcgagct
     2281 agaacccaac aaagaaacgt acacgctgct gctgcaggct taccaggaac ggggcatgga
     2341 cgccgaagcc gacatgattg cagaaaagct aaaaaagttg attgtacctg ctggtcgtcc
     2401 acgacgcatt ttgcgtcccc gcagggttgt catttaaccc ctgttttggt acactaacgc
     2461 ggtagccggg ccaaaggaag tcgtgccgac atagctcagt ggctagagcg gctgttttgt
     2521 aaacagcggg tcgtgggttc gaatccctct gtcggctcca cgttcacaat aagtgctcgc
     2581 ctgggatttt ccctggaaaa gagcagccca agtcggcgga ttcattccgc gacctggctt
     2641 aggtgagaaa aacctccggt ttgtctcatt ctggttttgc agggttagtg aagcggtcaa
     2701 acacgggtga ctgtaaatca tctggctcag ccttcggggg ttcgaatccc tcaccctgca
     2761 ccaaatcgaa tttttttaaa gcacttctaa aagaggtgct ttaaattttg ccctaaattt
     2821 cttggaacgc attagacaga ggttaggact aattgtgagt agcactgaag ccatgctggc
     2881 catcgagcca atactagaca ccgtaccgca gtacgaagat tttttgacgt ttgcagaaat
     2941 ccgaagtcgg tcagcggtat tagccgatac atatccggat tcattgcgca tcgaaaccat
     3001 cggcgaatct gccgaaggac gtccgctgga actgttggtt gtaggtaaca cacagtcgct
     3061 agatacgttc gggccagagc agggccgccg aacagctctt atttttggag cgccgcaccc
     3121 taacgagccg agcggtgtcc acactagctt tgtgatggca gagtatttcg ccaaatatcc
     3181 cgaccgcttg caagactttg gcttcgatac attactggtc ttgccggccg ccgacgttga
     3241 cgggcttgtg ctgaacgagg atggttttaa gggcttttcg ccggaaaagt acctagagaa
     3301 tttctatcgt ccaccattta aggaccagcc ggaatggtcg ttcccgatca acattcctga
     3361 acttggactg ttctttgatc aacctatgcc cgtagccgcg gcgctggcga cagttatgga
     3421 tgcctggcga ccgcggtttg tcgggtcact ccataattcg tggaccgaaa ccggggcata
     3481 ttttacggct aaccgccaca tcggcaaatt ggcgggaata gtccaagagt tctccgaaag
     3541 gtatggcatt cccttgcatc aggggccaaa cgaacagccg tatggacaag agctgcaccc
     3601 aggagtgtac acgcatgctt cgcgtattgt tgaaacggtt tggaataaaa tccacggaaa
     3661 agccaatacg aaagaagccg ggggctccta tgactatcta ttgcggcatg cgctggcggg
     3721 caaaaactac caagccatga tcgaagcggg cacgctaaat ctcgacggtg aagccgaaga
     3781 tcatccca
//
LOCUS       DATNGP010000070         2214 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_2858288, whole genome shotgun sequence.
ACCESSION   DATNGP010000070 DATNGP010000000
VERSION     DATNGP010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2214
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2858288"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..926)
                     /locus_tag="VN778_01720"
     CDS             complement(<1..926)
                     /locus_tag="VN778_01720"
                     /inference="COORDINATES: protein motif:HMM:NF024991.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXR49724.1"
                     /translation="MIIRKDRLAAVKHWLQRAFPGVFRVGEDRIAEDPLLSDEQMAIY
                     RALESSESHAFITGKAGTGKSVVLRYFASHTKKVVVKVAPTGISSLAVQGQTIHSLFR
                     LSTGVLDPRSIQVSSETEEILRHVDVIVIDEISMVRADTIDAIDRILRISKANQLPFG
                     GTQMVMFGDVYQLPPVAVGLRLQNYFRAKYGGAYFFNAHVWKKTELTTYELQTVFRQA
                     DESFRDLLNEVRNGSYTKKTLTTLNSRVAALSDTPTDTIILTSTNKAAQEINEERLSR
                     LRGKTKSYSAVTTGNVPIGTLPTEKKLKLRIGA"
     gene            complement(937..1851)
                     /locus_tag="VN778_01725"
     CDS             complement(937..1851)
                     /locus_tag="VN778_01725"
                     /inference="COORDINATES: protein motif:HMM:NF014691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HXR49725.1"
                     /translation="MEYQTQLKSILQASGWTQEELARQLDVSFVTLNAWINGKSEPRK
                     KAQDAIQALYFDIVGFDSINQETLGIQKKAASRLHTTARAIAKDKAVLNKLILHLTYH
                     TNTIEGSTMTLADTEEVLFNNQVLANRTQIEQVEARNHQAALLWLLDGIQTKGFALTE
                     DIVKGLHLRLMNGILSDAGQYRRHSVRIMGTRVTVANYLKVPDLMADLMKNISNERQD
                     VITGLAQAHAVFEKIHPFSDGNGRVGRLIMLAQALQVGNIPPLVLKEKRRAYYKYLEM
                     AQTKEELGPLEFFIAESIQSSNHLLFDD"
     gene            complement(2016..2090)
                     /locus_tag="VN778_01730"
     tRNA            complement(2016..2090)
                     /locus_tag="VN778_01730"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2056..2058),aa:Gln,seq:ttg)
BASE COUNT          569 a          518 c          493 g          634 t
ORIGIN      
        1 gcgcctatac gcagctttaa ttttttctct gtaggtaatg tgcctatcgg cacattgccg
       61 gttgtcacgg ctgagtatga ctttgtctta cccctaagcc ttgagagtcg ttcttcatta
      121 atttcttgtg cagccttatt agtactcgtg agtatgattg tatccgtcgg tgtgtcggac
      181 aacgcggcta ctcgcgaatt aagtgtagtg agcgttttct tggtgtacga accatttcta
      241 acttcgttga gtaggtccct aaaactttca tccgcttgcc taaacacggt ctgtaactcg
      301 tatgttgtca gctctgtctt tttccaaaca tgagcattga agaaataagc accgccgtac
      361 ttagctcgga agtagttttg tagccgtaaa cccactgcaa caggaggaag ctgatacaca
      421 tccccaaaca tgaccatctg cgtgccacca aaaggcaatt ggttagcctt ggagatgcgt
      481 aaaatccggt caatcgcatc tatagtgtca gctcgaacca ttgatatctc atcaatgaca
      541 atcacatcga catgacgcag gatttcttcc gtttccgatg acacttgtat agacctaggg
      601 tctagtacgc cggtggacag tctaaacagt gagtgaatag tttgcccttg aacagccagc
      661 gacgatatac cggtgggcgc aaccttcaca accacctttt tggtatggct tgcaaagtat
      721 cgcaacacaa ctgatttacc ggtcccagcc ttacctgtga tgaatgcatg ggactcgcta
      781 ctttctagag cgcgatagat tgccatctgc tcatcactta gtagagggtc ctcagcaatc
      841 ctatcctcac ctacacgaaa tacgcctgga aaggctctct gtagccagtg cttcaccgcg
      901 gctagtctat ccttacggat aatcataaaa tgcagtttag tcatcaaata gcaaatggtt
      961 cgatgattgt atcgactccg ctataaagaa ttcaagcggc ccaagctctt cttttgtttg
     1021 tgccatctct agatacttat agtaagcacg tcgtttctct tttaatacaa ggggaggtat
     1081 gttgccgact tgcagagcct gagccagcat aatcaggcgc ccaacacgtc cattgccgtc
     1141 actaaaaggg tgaatcttct caaaaaccgc atgagcctga gccagaccag ttataacatc
     1201 ctgtcgctca ttagaaatat tcttcattag atcggccata aggtctggca cttttaggta
     1261 gttagccacc gtaacacgcg tacccataat tcgcacacta tgccgtcgat attgtcccgc
     1321 atcactcaat atgccattca taagacgcaa atgcaggccc ttaactatgt cttcagtcag
     1381 ggcaaagcct tttgtctgaa ttccatcgag taaccataga agtgcagctt ggtgattgcg
     1441 cgcttctact tgctctattt gagtacgatt ggcaaggact tgattgttga aaagtacctc
     1501 ttctgtgtcg gccagggtca tcgtgctacc ctcaatagta ttcgtgtgat aggttagatg
     1561 gaggattaac ttattaagca ccgctttatc cttggcaatg gctcgagcag tggtatgtag
     1621 tctgctggcg gctttcttct gaatacctaa tgtttcttga ttaatactgt cgaagccgac
     1681 aatatcaaag tagagagctt ggattgcatc ctgggccttt ttgcgcggct cggacttccc
     1741 gttaatccat gcattaagcg tgacaaaaga aacatcaagt tgacgagcga gctcttcctg
     1801 ggtccagccg cttgcttgaa gtatagattt taactgagtt tggtattcca taactttata
     1861 agtatatcaa aactttataa agttatcaat cacataggtg ctgatttttg agagcagagc
     1921 aaggcctacg tcatatggaa ttcgaactat tataacaggg gtaaaaaata aaaagacgtt
     1981 tgattccaaa cgcctttaca tctgtaatat tctcgtggct agggcggagg gattcgaacc
     2041 cccgaatgcc aggaccaaaa cctggtgcct taccacttgg cgacgcccta ttagctttgc
     2101 ccgattatac taaaaacttt gcggtaacgc caggggtgtg ctgtgtgcaa gtagccgacc
     2161 tagttttcta ggtagttgta gatttgccga gagagcgtcg tgagcggctg agtg
//
LOCUS       DATNGP010000071         9018 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_2242665, whole genome shotgun sequence.
ACCESSION   DATNGP010000071 DATNGP010000000
VERSION     DATNGP010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 9018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9018
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2242665"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..569)
                     /locus_tag="VN778_01735"
     CDS             complement(<1..569)
                     /locus_tag="VN778_01735"
                     /inference="COORDINATES: protein motif:HMM:NF017009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49726.1"
                     /translation="MSQDARLDDEQATQRRARVLGLEYIDTSQIVDKHLYKELLTVPE
                     LYQLKIIPLQADQSNILFGVTTTTSQQTINNLRQRFQDQRVSFAIISDTGYREYMRLY
                     DPPRQVVYHDITINQAGSTEMVEQISAMLEQVRADDMLAYLVAQAHRLNASDIHIETQ
                     PDYVRIRFRIDGVLHPIAHLNQDKYRVLI"
     gene            617..1111
                     /locus_tag="VN778_01740"
     CDS             617..1111
                     /locus_tag="VN778_01740"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HXR49727.1"
                     /translation="MRQLVLIAHNLRSTHNVGSLLRTADGLGVQQVWLTGYTPYPLVL
                     HDERLPHLAQKIDRHIIKTALGAEKTVSWQYKTDIKTVIKDLKHRGFTIAALEQVPNA
                     ISLPDFKPPEKLALVVGREVEGLEPEVVQMCDPSLEIPMFGSKESFNVVQAAAMALYH
                     FRFS"
     gene            1171..2088
                     /locus_tag="VN778_01745"
     CDS             1171..2088
                     /locus_tag="VN778_01745"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HXR49728.1"
                     /translation="MAIATRSKRSTPSSKKRSGKHHRHSKPYLKTYWPYLPMLLIVGI
                     GLAINSLWSAGSVLGSSSNFSSEALLSSTNHARTLDNESSLTIDPQLTAAAEAKANDM
                     VSRNYWAHNTPDGKTPWTFIIAAGYQYQAAGENLAYGFDNAADTVTGWMNSPEHRANI
                     LNTSYQNVGFGVASSPNYQGKGPETVVVAEYGEPATAVANITFTVPNPVGTAGAQDTS
                     LSNKELSAQPVSRIQLLTGGKATWATMVASAITGAALAVFLIRHWFHLKRLVSRSELF
                     IVHHPWLDIAATAIFTAGFILTRTSGLIR"
     gene            complement(2093..5734)
                     /locus_tag="VN778_01750"
     CDS             complement(2093..5734)
                     /locus_tag="VN778_01750"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF005298.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HXR49729.1"
                     /translation="MAANTKLKPSDFVHLHNHTQYSLLDGLTKVPALVERVKKSGMTA
                     VAMTDHGTLSGAIEFYKEAQAAGIKPIIGMETYVAARSLYDKDPGKDKPNFHLILLAM
                     NQAGYQNLMALSTIANLEGFYYKPRIDRELLASHNEGLICLSGCINGEVGDKLRSGDY
                     KGAKKIAQWYKEVFGDRYYLELQDHGHPEHPSKWAEQVPVNAQLLELGKELDIPCVVT
                     CDAHYLKHEDQEAHEILLCVQTSSFLSDEKRMSLKEFELHVTEPHEVVERWGKEHPEL
                     IRSSRAIADRCDVNIELGKILIPKFPVPKGETEKSLLHKLVWQGLAWRYADIELSESS
                     KLTEAAAKKLLSKEIILRAEYELEIMDSMGFNGYFLIVQDFIKWGKDQGIIFGPGRGS
                     AAGSIVAYAVRITEIEPLKYNLMFERFLNPGRISMPDIDIDIQDSRRDEVIQYCVEKY
                     GKERVANIVTFGRMAARNAVRDVSRVLQVPYAEADRLAKMIPPPVQGRHVPLATALKE
                     DRELKQEYETNETAKKMFDLAMQLEGTIRSHGVHAAGVVIAPEDIVFYTPLEMAQKGV
                     IATQYSMGPIEELGLLKMDFLGLSNLTILNNALRIVRKVYQNDLALSEIPNDDPKTFE
                     LFQQGDTTGVFQFESAGMKRYMKELRPTLFEDLIAMNALYRPGPMAEIPRFIEAKNSA
                     GPIRYFHSSLEPILKETYGVMVYQEHIIQLLQLVAGYSAGEADLVRKAIGKKKRDIMQ
                     AEEPKFITGCVKQGLTEAQAHQLWKQIQPFADYSFNKAHATCYAQIAYWTAYLKANYP
                     AAFMAALMTSDYDDNDRLAIEITECKHMGITVLPPDVNESFVEFAVVPETEQIRFGMT
                     AIKNVGTGAVEEILRARADSTFASLEDFLTRVSCRIVNRKAMESLIKAGAFDRFGDRG
                     TLLYNLDLVLAFAARVQKQANSGQTDIFGTLLEETSAHKPKLELLTPPEMSDSREQLL
                     WERELLGLYLSQHPLELFETILAEKAVPLNSLTIEHDNKSVSVGGAVTDVREITTKNG
                     QKMAFVKLEDQSGEIEAILFPSSYQHTIGLWERDRVVLIKGKVNGRDKEGNKTTELKV
                     MVDDAREITAEQAAAYQATGKKVKAPPKKTSSVGARATQSLKNKATNPELKSPRIYIR
                     LQSTSDERTLLTLKETIDTHHGDTEVVLVLGEATQKQAIKLPGGIDSASDGLERLREL
                     VGPKNLVVK"
     gene            complement(5798..6217)
                     /locus_tag="VN778_01755"
     CDS             complement(5798..6217)
                     /locus_tag="VN778_01755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HXR49730.1"
                     /translation="MIGRDKKRSNFKRLAIGSALAATAGYLAGVLTAPKSGKETRSDL
                     KTAADKSWAEAEKDLKKLNKELGKVIDDAKGRGGKLSKKAQKELEATVDKAKDAKEKV
                     REMISAVREGDAQDKDLDRAVKDATTAIAHLRDYLKK"
     gene            complement(6218..6469)
                     /locus_tag="VN778_01760"
     CDS             complement(6218..6469)
                     /locus_tag="VN778_01760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49731.1"
                     /translation="MTTTDQVLLIILTVLLSVFFLLLIAMAAISFKLLNEARKVVAKA
                     NDVADSVADAAEAFRDVQGRMALFKLIRNIVKLAKKRGR"
     gene            complement(6498..7397)
                     /locus_tag="VN778_01765"
     CDS             complement(6498..7397)
                     /locus_tag="VN778_01765"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HXR49732.1"
                     /translation="MSQPKVRKAVIAAAGFGTRFLPQTKAIAKEMLPLVDKPIIQYIV
                     EDLVEAGICDVIIVTGYTKRNIEDHFDTPNEDLLNNLRAGGPSKTHYIEEVNAIADMA
                     NFAYVRQKGPYGTATPIMNVAHLIGSEPFIYAFADDLTVAKPNAFQQMIDLYTETGSS
                     ILHCIQATDDKDYDRYGFVGGDQVREGVLKIDSIVEKPGKAAAPSNFASVGSHLLTPE
                     VFNYLEAGKHNLKSGQEFYLTDAVLQPMLADGKVFYGAEIKNSARYDTGNPLDYLKTV
                     MDFALERPDIGPELADYLRKKLQ"
     gene            complement(7514..8932)
                     /gene="gatB"
                     /locus_tag="VN778_01770"
     CDS             complement(7514..8932)
                     /gene="gatB"
                     /locus_tag="VN778_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HXR49733.1"
                     /translation="MISSETRAKYRATIGIECHVQLKTKTKLFSAVGNDAREAPPNTL
                     VSHIDFGLPGALPVLNGEALHLATRAAFALDTEPARFSKFDRKHYFYPDLPKGYQISQ
                     FDEPIIGKGSIVIEVDGEEKTIGITRAHLEEDAGKSVHPDGKDYSLVDLNRAGTPLLE
                     IVSEPDLHTGAEAKAYARELYLRMRYADVSEADLYYGNMRFDVNVSVSQTDELGTRSE
                     TKNLNSFRAVEKAVNYEINRQIELLEKGGRVVQETRGWDDAKQKTFSQRTKENADDYR
                     YMPDPDLPPIVLDNEYVAKIKGEMPKLPPEIRKELSKYDLGAQVIEDILDNPDIHKKL
                     SEVIAGGTAAENIRRIAYDSLEKLDTLKIPAEAHVKVAESVRANILNSTSATEILDAL
                     LENAKQDVDELIQGNAQVSDEGEIAKIVAQVLSGNAKAAEDVKNGEMKAIGFLVGQVM
                     KLSQGKANPQMAQELIKKQLGL"
BASE COUNT         2108 a         2337 c         2153 g         2420 t
ORIGIN      
        1 ctgatgagta ctcggtattt gtcttgattt aaatgagcaa ttggatgcaa cacaccatcg
       61 atacgaaaac gtatgcgcac gtaatcgggc tgcgtttcta tatgaatgtc tgaagcattt
      121 aggcggtggg cttgggcgac cagataggct aacatgtcat ccgctcgcac ctgttccagc
      181 atcgcggaaa tttgctctac catttccgta ctgcctgcct gattaatagt tatgtcgtga
      241 taaacaacct gcctcggcgg atcgtataag cgcatgtact cccgataacc cgtatctgaa
      301 atgatggcga agctgacgcg ctgatcttga aagcgctgcc taagattatt gatagtttgt
      361 tgagaggtgg tcgtagtaac gccgaagaga atgttgctct gatcggcttg tagcggaata
      421 attttcagct gataaagctc ggggacggtc aaaagctcct tataaagatg cttatctact
      481 atctgggagg tgtcaatgta ctccaaaccg agcacccgag cccggcgctg ggtagcttgc
      541 tcatcgtcca gtcgagcatc ttgactcatt cctttgatta taaagcataa gcgtagctct
      601 agggtatagt ttaagcatgc gacaattggt cttgatagca cataatctgc gcagtacgca
      661 caatgttggc tcacttctgc gtacagccga tgggctgggt gttcaacaag tctggctgac
      721 gggatatact ccttacccac tggttttgca cgatgaacga ttacctcatc tagctcaaaa
      781 aattgatcgg cacatcatca aaactgcttt aggtgcggaa aaaacagtta gctggcagta
      841 caaaacagac attaagaccg ttatcaagga cttgaagcat cggggtttta cgattgccgc
      901 gctcgagcaa gtgccaaatg ccatttctct accagatttt aagccacctg aaaagttagc
      961 tctcgtcgtc ggtcgggaag ttgagggcct tgagccggaa gttgtacaaa tgtgcgaccc
     1021 cagtctggaa ataccaatgt tcggcagcaa agaatctttt aatgtcgtcc aagcggccgc
     1081 tatggcctta taccattttc gctttagcta atcgcgattg gttacgctta tggtatgcta
     1141 taatgcagat tagaacctaa aaacaaaaat atggctatcg ccaccagatc aaaacgcagc
     1201 actccctcgt ctaaaaaacg cagcggcaaa catcatcgcc acagcaagcc gtatctcaag
     1261 acgtactggc cgtatctacc gatgctgctg atcgtcggca tcgggcttgc tatcaatagc
     1321 ttatggagtg cggggagcgt gttgggcagt agcagcaatt tcagcagcga ggccctgctc
     1381 tccagcacta atcacgcccg gacgctcgac aatgaatcat ccttaaccat cgacccccag
     1441 ctgactgccg cggctgaggc taaggccaac gatatggtga gccgcaacta ttgggctcat
     1501 aatacccctg acggcaagac accctggact ttcatcatcg ctgctggcta ccaatatcaa
     1561 gcagcaggcg aaaacctggc ctatggcttc gacaatgcgg ctgatacagt gaccggttgg
     1621 atgaatagtc ccgagcaccg ggctaacata ctaaatacca gctatcaaaa cgttggtttt
     1681 ggcgtggcta gctcaccgaa ctatcaaggg aaaggcccgg agaccgtcgt cgttgccgag
     1741 tatggcgagc cagcaactgc ggtagccaac atcaccttta cggtaccaaa cccggttggc
     1801 acagctggcg ctcaagacac cagtttaagc aataaagagc tctcggctca gccagtatcg
     1861 cgcatccagc tacttaccgg aggcaaggct acctgggcga ctatggtcgc ctcagctatc
     1921 accggcgctg ccttagccgt tttcttgatc cgtcactggt tccaccttaa acgtcttgtt
     1981 agccgcagcg aacttttcat agtacaccac ccctggctgg acattgcagc cacggccatc
     2041 tttaccgccg gtttcattct gactcgcacc tcgggcctca tccgctaggc agttatttaa
     2101 caaccaagtt cttggggccc accagttcac gaagcctttc aagcccatca cttgcgctat
     2161 cgatgccacc cggaagcttg atggcttgct tctgggtagc ttcgccaaga acgagcacaa
     2221 cctcagtgtc accgtgatgg gtatcgatag tttctttaag ggttaagagc gtgcgctcat
     2281 cactcgtgct ttgcaggcga atataaatcc ttggggattt tagttctgga ttcgttgctt
     2341 tgttctttag gctttgcgtg gctcgagctc ctacactaga agtctttttt ggaggtgctt
     2401 tgactttttt tccggttgct tgataagcgg cagcttgttc tgccgtaatt tctctggcgt
     2461 cgtcgaccat aacctttagt tcggtggtct tgttgccttc tttgtcgcgg ccattcacct
     2521 tgcccttgat cagtacaaca cgatcacgtt cccaaaggcc tatggtgtgt tgatagctgc
     2581 ttggaaagag tatggcttcg atttcgccag attggtcttc gagcttaaca aaagccattt
     2641 tttgaccatt tttggtagtg atttcccgta catcagtgac tgcaccaccg acactgactg
     2701 atttgttgtc gtgctcaatc gttaaactgt tcagcggtac cgctttctcg gcaagaatgg
     2761 tttcaaaaag ctcaaggggg tgctgactga gatataaacc aagcagctcc ctttcccata
     2821 acagttgttc acggctatca ctcatttcgg gcggtgtcag tagctccagc tttggtttgt
     2881 gcgccgacgt ttcttccagt agagtgccaa aaatgtccgt ctgcccacta ttagcctgct
     2941 tctgtacgcg ggccgcgaaa gccagtacca gatccaagtt atataacagc gtccctcgat
     3001 caccgaagcg gtcaaaggcg ccagccttga tcagactctc cattgccttc cggttgacaa
     3061 tacgacagct aacgcgggtg agaaaatctt cgagactcgc aaaagtactg tcagcgcggg
     3121 ctcgcaagat ttcctcgacg gcaccggtgc caacattttt gatagcggtc atgccgaagc
     3181 gaatctgctc tgtttccggt accaccgcaa actcgacaaa cgactcattg acgtccggcg
     3241 gtaacactgt gatacccatg tgtttacatt cagtaatttc aattgctaaa cgatcgttgt
     3301 catcgtagtc gctcgtcatc agagcggcca taaaggcagc tggataattt gcttttagat
     3361 aggcggtcca ataggcaatt tgtgcataac acgtggcatg cgccttatta aaggagtagt
     3421 cggcgaaagg ctggatttgt ttccaaagtt gatgcgcctg ggcttcggtc aggccctgtt
     3481 taacacagcc ggtaatgaat ttcggctctt cggcctgcat aatgtcgcgc ttttttttac
     3541 caatcgcctt gcgcactaaa tccgcttcgc cggcactata gcccgccacc aattgcagca
     3601 gctgaatgat gtgctcctga taaaccataa cgccatacgt ttccttcaat atcggttcca
     3661 gcgaggagtg gaagtatcgg attggccctg cactgttttt ggcctcgatg aagcgtggaa
     3721 tctcagccat cggaccgggc cgatacaagg cattcatagc gatgaggtcc tcaaaaagcg
     3781 tcggacgcag ctctttcatg taccgcttca tgccggccga ctcaaactgg aagaccccag
     3841 tcgtgtcgcc ttgctggaag agttcaaagg tcttgggatc gtcattagga atctcactta
     3901 aggctaagtc gttttggtaa actttacgca caatgcgcag ggcattattg agaatagtaa
     3961 ggttggagag gccgagaaag tccattttca gcaaacctag ttcttcgatt gggcccatgg
     4021 aatactgcgt agcgataacg cctttttgag ccatttccaa cggcgtatag aagacgatgt
     4081 cctccggggc tattacgacg ccggcagcat gcacgccgtg gctgcggatg gtaccttcca
     4141 gctgcatggc taaatcaaac atcttcttag ccgtttcgtt cgtctcatac tcctgcttga
     4201 gttcacgatc ttctttgaga gcagtagcca gtggaacatg acggccttgc actggtggcg
     4261 gaatcatttt tgccagacga tcggcttcgg catagggcac ttgcaagact cgggatacgt
     4321 cacgaacagc gttgcgggca gccatgcgac caaaggtcac aatgttggcg acccgctcct
     4381 tgccatactt ttctacacaa tactgaatca cttcatcacg acggctgtcc tgaatgtcaa
     4441 tgtcgatgtc gggcatacta atgcgtccag gatttaagaa ccgttcgaac ataagattgt
     4501 atttcagggg ctcgatttcc gtaatgcgca cggcataggc cacaattgaa ccggccgccg
     4561 aaccgcggcc agggccaaaa ataatacctt ggtctttacc ccatttaata aaatcctgga
     4621 caattaaaaa gtagccgtta aagcccatgg aatccataat ttccagctca tactcggccc
     4681 gtaagataat ttctttggac agtaattttt tagctgctgc ctctgtcagc ttactgcttt
     4741 cactcaattc tatgtcggca taacgccaag ccagaccttg ccacactaac ttgtgcagca
     4801 atgacttctc ggtctcacct ttcggcaccg gaaacttcgg aataagaatc ttgccaagtt
     4861 cgatatttac gtcgcatcgg tcggcaatgg cacgcgagct gcgaataagc tcggggtgtt
     4921 ctttccccca acgctcgaca acttcatgag gctcggtgac atgcagttca aactccttga
     4981 gactcatacg cttttcgtcg ctcagaaacg agctcgtttg cacgcacagt agaatctcgt
     5041 gcgcttcctg gtcttcatgc ttgagataat gagcgtcaca agtcacgacg cacgggatat
     5101 ccagctcttt cccaagctcc agcagctggg cattgacggg cacttgctca gcccatttgc
     5161 tggggtgctc gggatgaccg tggtcctgca gctccagata atagcgatcg ccaaatactt
     5221 ccttatacca ctgggcaatt ttcttggcgc ctttataatc gccactccgt agcttgtcgc
     5281 caacttcacc attgatacaa ccgctgagac aaataagtcc ctcgttgtga ctggccagca
     5341 gctcccggtc gatccgcggc ttgtaataaa agccctctaa attggcgatg gtactgagtg
     5401 ccatcagatt ctggtagcca gcttggttca tggccagcaa aataaggtga aaatttggct
     5461 tatccttgcc ggggtcttta tcgtacaagc tgcgtgccgc cacatacgtc tccataccga
     5521 taataggttt aatgccggcc gcctgagctt ccttataaaa ctcaatggca ccgctcagtg
     5581 tgccgtggtc ggtcatcgcc actgccgtca tgccactctt tttgacccgc tcaacaagcg
     5641 ctggtacctt agtcaaacca tcaagcaggc tatactgtgt atggttgtgc aggtgcacaa
     5701 aatcgctagg cttaagcttg gtgttggccg ccaaaatatc cctcgagaat agttagtgct
     5761 atttacgtaa ttatagcact aaagcactgt ccgctgctta tttcttcaga taatcgcgta
     5821 gatgggcgat agcggtagta gcatctttga cggcccggtc gaggtccttg tcctgggcgt
     5881 cgccttcgcg gacggcgctg atcatttcac ggactttttc tttggcatcc ttagccttgt
     5941 cgacggtcgc ttcgagttct ttttgagctt ttttgctgag tttgccgccg cgccctttgg
     6001 catcgtcgat gacttttccg agttctttat tcagcttctt caggtctttc tcggcttccg
     6061 cccagctttt gtcagcggcg gttttgagat cgcttcgagt ttccttgccg cttttaggcg
     6121 ccgtcagcac accggctaga tagcctgccg tagcagccag agcgctgcca atagccagac
     6181 gtttgaaatt gctgcgcttt ttatccctac caatcatcta tcggcccctt ttcttagcga
     6241 gtttgacaat gttgcgtata agcttaaaaa gtgccatgcg tccctgaaca tcccggaaag
     6301 cttctgcagc atcggctacg gaatcagcta catcattagc tttggcgact accttgcggg
     6361 cttcgttgag aagcttgaat gagatggcag ccattgcgat taaaagcaag aaaaagacac
     6421 ttaagaggac ggtcaggata atgagaagga cttggtctgt agtagtcata atttcagtat
     6481 atagctattg ttataatcta ttgtaacttt tttcgcaaat agtctgccag ctccggcccg
     6541 atatcaggac gttccagcgc aaaatccatc acagttttta agtagtccag cgggttgccg
     6601 gtatcgtaac gagcactatt tttaatttcg gctccgtaaa aaactttgcc gtcagccagc
     6661 ataggctgca gtactgcatc ggtcagatag aattcttgac cgctctttag attgtgcttg
     6721 ccggcttcca gatagttaaa aacctccggc gtgagtaaat ggctcccgac actagcgaag
     6781 ttagaaggag cagcggcctt gcctggtttc tctacaatgc tgtcgatctt gagtacaccc
     6841 tcgcgtacct ggtcgccccc cacaaaaccg tatcgatcat aatccttgtc atcagtagcc
     6901 tggatgcaat gtaaaatact gctgcccgtt tctgtataga gatcaatcat ttgctgaaaa
     6961 gcatttggtt tcgctaccgt aaggtcgtcg gcaaaagcat agataaacgg ctcgctaccg
     7021 ataaggtgag ctacattcat gatgggtgtg gctgtgccgt acggcccctt ttgacggaca
     7081 taggcgaagt tggccatgtc ggcaatggcg ttcacttctt cgatgtagtg ggttttgctt
     7141 ggtccgccgg cacggaggtt attcaataaa tcctcgttcg gagtgtcaaa atggtcttca
     7201 atatttcttt tagtgtagcc cgtgacaata ataacgtcgc agatgccggc ttcgaccaga
     7261 tcttccacga tgtactggat gatcggtttg tcgacgagcg gcaacatctc tttagcgatg
     7321 gctttcgttt gcggcaaaaa tcgcgtcccg aaaccggctg cggcaataac tgctttacga
     7381 acttttggct ggctcattgt ccatcgattg tatcatgaac ccctggtcgt aacaggccga
     7441 aaccaggcct gcttgccata ggtagtttga ccgcaagtat caaaggccct aaaggcacac
     7501 gactcgccaa cagctaaagc ccaagttgtt ttttgataag ctcttgggcc atctggggat
     7561 tagccttgcc ctgggacagc ttcataactt gcccgacgag gaagccgatg gctttcattt
     7621 caccattttt cacatcctca gccgccttgg cgttgccgct gagcacttgc gcgacgatct
     7681 ttgcaatctc gccttcatct gagacctgtg catttccttg aataagctca tcgacatctt
     7741 gctttgcatt ctcgagcaaa gcatctaata tctcagtagc tgatgtggaa tttaagatgt
     7801 tggcccgtac actttcagca actttgacat gagcttccgc gggaatttta agcgtatcaa
     7861 gcttttccaa agaatcgtaa gcaatgcggc gaatattttc cgccgctgta cccccggcaa
     7921 ttacttctga tagtttttta tgaatgtctg gattatctaa aatatcttca attacttgag
     7981 cgccaagatc gtacttcgat agttctttac gaatttcggg cggcagtttg ggcatctcgc
     8041 ctttgatttt cgcaacatat tcgttatcaa ggacaattgg cggcaaatcc ggatccggca
     8101 tgtaacgata atcgtcggca ttttctttgg tgcgttggct gaaggtcttt tgtttggcat
     8161 catcccaacc acgcgtttcc tggacgactc gcccaccctt ttcaaggagc tcaatttgcc
     8221 gatttatctc ataattcacg gccttctcaa ctgcccggaa gctattgagg tttttggtct
     8281 ccgaccgcgt ccccaactcg tccgtttgac tgacgctaac attcacatcg aaacgcatat
     8341 tgccgtagta taggtcggcc tccgagacgt cggcgtagcg cattcggaga tacagctcac
     8401 gggcataagc tttggcttcg gcgccggtgt gtaagtccgg ctcgctaacg atttcaagca
     8461 gcggcgtacc ggcacggttc aaatcaacca agctataatc cttgccgtca gggtgcacac
     8521 ttttaccggc atcttcttcc aggtgcgccc gggtgatccc gatggtcttt tcttcgccat
     8581 caacctcaat gacaattgat cctttaccaa taataggttc atcgaactga ctgatttggt
     8641 agcccttggg caagtcagga taaaaataat gtttgcgatc gaatttgcta aaacgggcag
     8701 gttcggtatc aagtgcaaag gctgcacgag tcgccagatg cagcgcttcg ccattgagca
     8761 ctggcagagc acctggcaag ccaaaatcaa tatggctcac caaagtgttg ggtggcgctt
     8821 cccgcgcatc gttaccaacc gctgagaaca atttggtctt ggtcttgagc tgcacatggc
     8881 actcaatgcc gatagtcgcc ctatatttag cgcgagtttc ggaactaatc atcgtttggc
     8941 acctccagga cacctagatc tcggagtgct tgccgaaaac cttgcagcgc ttctagcaca
     9001 tcttgcttac ggagcttg
//
LOCUS       DATNGP010000072         3686 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_1554771, whole genome shotgun sequence.
ACCESSION   DATNGP010000072 DATNGP010000000
VERSION     DATNGP010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3686
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1554771"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3640
                     /gene="rpoC"
                     /locus_tag="VN778_01775"
     CDS             <1..3640
                     /gene="rpoC"
                     /locus_tag="VN778_01775"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HXR49734.1"
                     /translation="RSREAAVDKNGELVTRSIVRRERMGHINLAVPVSHIWFLRGTPS
                     AMGLLLGMTVKNLERVAYFASYIIKSVDVEKRNQLLTDKEAEFAAAKEAIKLRYEKEA
                     EAENANVKALAEMQTKETEEVTKEFETLREKLVGLERFHLINETDYRNLPDDLRALIT
                     VGMGGAALRDLLQEIDLKQLISDLTKEAEDAKGQRKKKLMKRLRLLESMDRAGIKPSS
                     MCVSVLPVIPPDLRPMVQLTGGRFATSDLNDLYRRVINRNNRLKKLIDLNAPEVIRRN
                     EQRMLQEAVDALIDNNNARSGRAVAATGQRRRLKSLSDMLKGKQGRFRQNLLGKRVDY
                     SGRSVIVAGPNLKINQCGLPKMMALELFKPFVIGELIEREQAHNIRSASRLIEMGETV
                     VWDALDEVIKGKYVLLNRAPSLHRLSIQAFQPVLIEGRAIQLHPLVCKGFNADFDGDQ
                     MAVHLPLSEKAQEEAREIMAANRNLLKPADGSPILHIEQDIVLGCYYLTYTRPGADAT
                     KVSTLSGLDEAIMALDAGGLALQDYVRVPFRGEVRQTTLGRLLFNEVFPEDFAFQDET
                     MTKKRLKQVMADVYSRYGQERAAEIADDLKDLGFHYATDSGLSMGMSDFQPIKGMDKL
                     LDEGEERAAAISDQYEAGFITDVERHRLTVDNWTKIETQVQGMLTEQMVGQDSSTAIS
                     INSGARGNISQLNHAVGMLGVLQDAGGNVIELPIKSGYIVGLNPLEYFTGTRGTRKAL
                     IDIALKTADAGYLTRRLVDVSQDVFTLEETTTDPGFAMRRSDAAEIGVSYASRLVGRF
                     VAETVKGYAKKGELITQELADKLDGDSAVDGLKIMSALSCTSVRGVAQKSYGIDPATG
                     ELVAEHHPIGVIAAQSIGELGTQLSLDSKHRSGSVVADDAAQGLSRVEELFEVRTPKG
                     QAYLTEISGVANAWEEGDRYVVQVTADAAEKVTLSLGERKATIKSGTDVVAGDVVAAL
                     EDASEPLTAPMAGKAQVTNKAVVITPTKQSVVRYEIPGFKQLLVQDGDKVVAGQRLTN
                     GSINLHDLMRLQGVEATQRYIMNEILKIFAAQGQTVADKHLEIIVRQMFSRVQIEEAG
                     DSEFVTGDIVSKLAVVEANEGLVASGKQPAKYNQLLLGITKASLSTDSFLSAASFQDT
                     TRVLIGAATSGKVDRLYGLKENVILGRKIPVGTGAKNEGELDDETAELQAEADQIIGA
                     TAAADAES"
BASE COUNT          887 a          940 c         1042 g          817 t
ORIGIN      
        1 tcgcagccgt gaagccgccg tcgacaagaa cggtgagctg gtaacgcgca gcatcgtccg
       61 gcgcgagcgc atgggccata tcaatttggc tgtgcctgtg tcgcatatct ggttcctgcg
      121 cggcacgcca agtgccatgg gcctgctcct cggcatgacc gtcaaaaacc tggagcgggt
      181 cgcatatttt gctagctaca tcatcaagtc ggtggacgtt gagaagcgca atcagctatt
      241 gactgacaaa gaggctgagt tcgcagccgc taaggaagct atcaaactgc gctacgaaaa
      301 agaagcggaa gccgagaatg ccaatgttaa agcactggcc gaaatgcaaa ccaaagaaac
      361 tgaagaagtc acaaaagagt ttgaaacgct gcgtgaaaag ctggtgggtc tggagcgctt
      421 ccatctcatc aacgaaaccg attaccgtaa tttgccggat gatctacggg cgctcataac
      481 ggttggcatg ggtggtgcgg ctctccgcga tctgctacag gaaattgacc tgaaacaact
      541 catcagcgat ctcaccaaag aagctgaaga cgcgaaaggt cagcgcaaga agaagcttat
      601 gaagcgcctg cgcttgctgg aaagtatgga ccgcgccggt atcaagccaa gctccatgtg
      661 tgtcagtgtc ttgccggtga ttccgcccga cctgaggccg atggtgcagt tgaccggcgg
      721 tcgtttcgcc accagtgacc tcaacgatct atatcgccgg gtcatcaatc gcaacaatcg
      781 cttaaagaag cttatcgacc tcaatgctcc ggaagtcatt cgacgcaacg agcagcgcat
      841 gcttcaggaa gctgttgacg ctttgatcga taataataat gcccgttcgg gccgcgccgt
      901 ggccgccacc ggtcaacgtc gccgtctgaa gagcttgagc gatatgctca agggtaagca
      961 aggccgcttc cgtcagaatc tgcttggtaa gcgggtcgac tattctggcc gttcagtgat
     1021 tgtcgccggt ccgaatttga agatcaatca gtgtggtttg ccaaagatga tggccttgga
     1081 gcttttcaag ccgtttgtta ttggtgaact cattgaacgt gagcaagccc acaatattcg
     1141 gagcgctagt cgactgatcg agatgggcga aacggtcgtg tgggatgctt tggatgaagt
     1201 gattaagggt aaatatgtat tgctgaaccg ggccccatcg ctgcaccggc tcagtatcca
     1261 ggcttttcag ccagtgctta tcgaaggccg cgctatccag ctgcatcctt tggtatgtaa
     1321 gggctttaat gctgacttcg acggtgacca gatggctgtc catttgccgc tgagcgaaaa
     1381 ggctcaagaa gaggctcgtg agattatggc tgctaaccgc aacttgctga agcccgctga
     1441 cggctcgccc atcctgcaca ttgagcaaga catcgtgctt ggttgctact acctgaccta
     1501 cacccggccg ggggctgatg caacaaaagt ttcgacactt tcagggctcg atgaagccat
     1561 tatggcactc gatgccggtg gtctcgcttt acaggattat gtgcgtgtgc cgttccgtgg
     1621 cgaggtacgt caaaccacgc ttggccgcct cttgtttaat gaggtctttc ctgaagattt
     1681 tgccttccag gacgaaacca tgaccaaaaa gcggcttaag caagtcatgg cagacgttta
     1741 cagtcggtat ggccaagaac gtgcagctga gattgccgat gacctcaaag accttggctt
     1801 ccactatgcc accgactccg gactgagcat gggcatgagc gacttccagc ccatcaaagg
     1861 catggacaaa ttgcttgacg agggtgaaga gcgggctgcc gcgatttctg accagtacga
     1921 agccggtttc ataaccgacg ttgaacgcca tcgtttgacc gtcgataatt ggactaaaat
     1981 tgagacgcag gtgcaaggta tgctgaccga acaaatggtt ggacaggact cttcaaccgc
     2041 aatctccatc aactccggtg cccgcggtaa catctctcag cttaaccacg ctgttggtat
     2101 gctgggcgtg ctgcaagacg ctggcggtaa cgttattgag ctgcctatta agtccggtta
     2161 tattgttggc ctgaacccgc tcgaatattt tactggtact cgcggtaccc gaaaggcgtt
     2221 gatcgatatt gccctgaaaa cggcggatgc cggatacttg acccggcgcc tggtggatgt
     2281 ctctcaagat gtctttacgc tcgaagagac taccaccgat cctggctttg cgatgcgcag
     2341 gagtgatgct gctgaaatcg gtgtttccta cgcttcacgc ttggttggtc gctttgtggc
     2401 tgaaactgtc aaaggctatg ccaagaaggg tgagctcatc acccaagagc tggcggataa
     2461 gctcgatggt gacagtgccg ttgatggcct caagatcatg agcgccttga gctgcaccag
     2521 cgtgcgtgga gttgcccaga agtcttacgg catcgatccg gcaaccggtg aactggttgc
     2581 tgagcaccat cccatcggcg ttattgcggc tcagagtatc ggcgagctcg gtacgcagct
     2641 gagtttggac agtaagcacc gctccggtag cgtggtggcc gacgacgccg cccaaggctt
     2701 aagccgtgtc gaagagctct ttgaagtccg cactccaaag ggtcaggcct atttgaccga
     2761 aatttcgggc gtggctaatg cttgggaaga aggtgatcgc tatgtcgtgc aggtaactgc
     2821 cgatgccgcc gaaaaagtga cattgagttt aggcgagcgt aaggccacta ttaaaagtgg
     2881 tacggatgta gtggccggcg atgtcgtcgc cgccctagaa gatgcctcgg agccactgac
     2941 agcgccgatg gccggcaagg cccaggtgac caataaagcg gttgtcatca cgccgactaa
     3001 acaaagtgtc gttcgctacg aaatcccagg ctttaagcaa ttgctcgtcc aagacggtga
     3061 caaagttgta gccggccagc gcctgaccaa tggttcaatt aacctgcatg atctgatgcg
     3121 tttgcaaggc gtcgaagcca cccagcgcta catcatgaac gaaattctga agatctttgc
     3181 ggctcagggc caaacggtgg ctgacaaaca ccttgagatc atcgtccgac agatgtttag
     3241 ccgtgtgcaa atcgaggaag ccggtgacag cgaattcgta accggcgaca tcgtcagcaa
     3301 actggcggtt gtcgaagcga acgaagggtt agtggctagc ggcaaacagc ctgctaagta
     3361 caaccagtta ttgcttggca ttactaaggc ctccctcagc accgactcct tcctgagtgc
     3421 cgcctccttc caggatacga cgcgcgtctt gatcggcgcc gccaccagcg gtaaagttga
     3481 tcgcctgtac gggttgaagg aaaatgtcat cttgggacgc aagattccag ttggcaccgg
     3541 cgctaagaat gagggtgaac tcgatgacga aacggcggag ctgcaagctg aagctgatca
     3601 gataattggg gctacagccg cagctgatgc cgaaagctaa agagctattg ctagattgag
     3661 ttcaatctgt tacactaagg aaaata
//
LOCUS       DATNGP010000073         4229 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_1921381, whole genome shotgun sequence.
ACCESSION   DATNGP010000073 DATNGP010000000
VERSION     DATNGP010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4229
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1921381"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..260
                     /locus_tag="VN778_01780"
     CDS             <1..260
                     /locus_tag="VN778_01780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49735.1"
                     /translation="TVFHMIAIWTQEDHMPPIHVNEFAEGGVLVNFQRAHELAAAAST
                     ADMHGSRLCGFFGFFAKTTQIFHRCRTISYFNPFVNSRYLL"
     gene            270..1625
                     /gene="der"
                     /locus_tag="VN778_01785"
     CDS             270..1625
                     /gene="der"
                     /locus_tag="VN778_01785"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HXR49736.1"
                     /translation="MSKKLPIVAIVGRANVGKSSLFNAILNRREAIVAREAGTTRDSI
                     MAKAEHGDRQFWLVDTAGIKDPEDDFEFTIQEQILQAADSADVIWVIVEADVPITEED
                     RRVAKLALRSQKPVFLVINKIDRAKGADLGSFERLGIKTMLQTAATHSKGIDLLLDRL
                     GEVIPKASQQEDDDRLHIAILGRPNVGKSQLFNALAKKQQAIVADRAGTTRDINRAVV
                     RYEGREIELLDTAGIRRSGKIEVGIERFSVIRALSAIEQADVCLLLMDVNELNVHLDQ
                     NIAGMIKDAGKGLILVVSKWDAAEDKDAYTRDALAPQIVANYDFVPWAPLIFTSAVTG
                     QNVTKLFDLALDINLERAKRISTSELNKWLRRAVDDHPPAGLKNRTPKLNYMVQETDN
                     PIPAFKIFGAQTKYVHWSYRRYLERRLREAYGYAGTPVQLWFIEKRLTHKHGVSPTKP
                     L"
     gene            1677..2318
                     /gene="pth"
                     /locus_tag="VN778_01790"
     CDS             1677..2318
                     /gene="pth"
                     /locus_tag="VN778_01790"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HXR49737.1"
                     /translation="MAWLQKRPQVSDPTMFYTVGKSRTVLLVGLGNPGKEYDLTRHNV
                     GFQCLDEFVRKADDMSDWIQKKDLKCLLSSGQLGESRVIAIKPTTFMNLSGEAVLAAV
                     NFYKISPDRIVVLHDELDIDFGQIRLRVGGSSAGHNGIKSVTQALGEDYGRIRIGVGP
                     KKPDRIKSEDFVLQKFSSDEQAQLPNLLKEVSAVLSEFIYGGQLSHETRNFLV"
     gene            complement(2315..2677)
                     /locus_tag="VN778_01795"
     CDS             complement(2315..2677)
                     /locus_tag="VN778_01795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49738.1"
                     /translation="MEKLRIEGGLVYLTEEQERVTGLPNPTSLDILRNNPFLIQGGIK
                     AREEELAAPHDLDVDVRSGEGLLEVMYGENHHEATSGDRLSKALDIAEDKELVIKGIV
                     EQLAPYLIDVPITNEHND"
     gene            complement(2723..3070)
                     /locus_tag="VN778_01800"
     CDS             complement(2723..3070)
                     /locus_tag="VN778_01800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49739.1"
                     /translation="MDHEDGRLIFSETEYFNVPELKPEMPEAETQVALAAVASRLGGV
                     RDEIEAIGADARACYRGRRTELRGLQAAVSRTCLQLTHVLEPTLSSRALVEADSEQVQ
                     AQLLGELKPYLER"
     gene            complement(3117..3482)
                     /locus_tag="VN778_01805"
     CDS             complement(3117..3482)
                     /locus_tag="VN778_01805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49740.1"
                     /translation="MDFDPSNSRLYFTKLERYLLGIGQDKAFPLYDVDDLLDDALNTR
                     EALVDGNGPEADLEIVENLIVELGSLQEYVAAPRSPRLTHDPARFAGCAALSGDTFTN
                     RQSMRRHRNAFTRSTHEDC"
     gene            3948..>4229
                     /locus_tag="VN778_01810"
     CDS             3948..>4229
                     /locus_tag="VN778_01810"
                     /inference="COORDINATES: protein motif:HMM:NF014533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HXR49741.1"
                     /translation="MIINKLTVNTVSIPPVLQKLSSPPKQLFHAGSPLAPLLERPRVA
                     IVGSRNISPYGREVTYRLARQLADQGIVIVSGLAFGVDNIAHQATLEASG"
BASE COUNT         1063 a         1133 c         1043 g          990 t
ORIGIN      
        1 acaccgtctt ccacatgatc gcaatctgga cacaagagga tcatatgcca ccgatccatg
       61 tcaacgagtt cgcagaaggg ggtgtacttg tcaattttca acgggctcac gagctcgcag
      121 ccgcagccag caccgcagat atgcatgggt cgcggctctg cgggtttttc gggttttttg
      181 ctaaaacgac ccagatattc catagatgtc gcactatatc ctactttaat cctttcgtca
      241 atagccgtta tctgttataa ttataagaag tgagtaaaaa attaccaatc gtagctatag
      301 tcgggcgggc aaatgttggc aaatccagct tgtttaatgc gatcttgaat cgccgtgaag
      361 ccattgtggc gcgcgaagcg ggtaccacgc gagacagcat catggccaag gccgaacacg
      421 gcgatcggca attctggctg gtggacactg ccgggatcaa agatcccgag gatgatttcg
      481 aattcaccat ccaggagcag atcttgcaag ctgccgacag tgccgatgtt atatgggtca
      541 tcgtcgaggc cgacgtcccg attaccgaag aagaccggcg ggttgccaag ctagcgctcc
      601 gttcacagaa accagtcttc ctggtcatta ataagatcga cagagccaag ggcgccgatc
      661 tcggtagttt tgagcgcctc ggcatcaaaa cgatgctcca gaccgccgcc acgcactcca
      721 aaggcataga cctgctccta gatcgtttgg gtgaagtcat cccaaaagca agccaacagg
      781 aggatgatga ccggctgcat atcgccatcc ttggtcgacc aaatgtcggc aaatcgcaac
      841 ttttcaacgc ccttgctaaa aagcagcagg ccattgtcgc cgatcgtgct ggtacgacgc
      901 gggatattaa ccgggccgtt gtccgctacg agggcagaga aatcgagctg ctcgacaccg
      961 ccggcatccg gcgcagcggc aagattgagg tcggcatcga gcgctttagc gtcatccgag
     1021 ctttgagtgc catcgaacaa gccgacgtct gcttactgct catggatgtt aacgagctca
     1081 acgttcatct cgatcaaaac atcgccggta tgatcaagga cgctggcaag gggctgatct
     1141 tggttgttag caaatgggat gccgccgagg acaaggatgc ctacactcgc gacgcgctgg
     1201 cgccacaaat cgtcgccaat tatgacttcg tcccctgggc acccctgatc tttaccagtg
     1261 ccgtaactgg ccaaaacgtc actaaactct tcgacttagc gctcgacatc aacttggagc
     1321 gcgccaaacg catctccacc agcgagctca acaagtggct ccgccgcgcc gtcgacgatc
     1381 atccgccggc cggcctcaaa aaccgtactc ctaagctgaa ctatatggtt caagaaaccg
     1441 acaaccctat ccccgccttc aaaatcttcg gcgcccaaac caaatacgtc cactggagct
     1501 accgccgcta cctcgagcgc cgcctccgcg aagcctatgg ttacgccggc acccccgtcc
     1561 agctctggtt catcgaaaaa cgcctgaccc acaaacacgg tgtaagcccc acaaagccct
     1621 tgtagggctt tgtgtagact cgagcgaagc acccgcgtat aagttataat tcatgcatgg
     1681 cctggctcca aaaacgaccg caagtgagtg atccgacgat gttttataca gtcgggaaga
     1741 gccgaacggt cttgctggtt ggcctaggca atcctggcaa agaatatgat ctgacacgac
     1801 acaatgtcgg ctttcaatgc ctcgatgaat ttgtgcgaaa agctgacgac atgtcagatt
     1861 ggatacagaa aaaggacttg aaatgcctac tcagtagcgg gcagctaggt gaatcgcgcg
     1921 tcatcgccat caagcccacg actttcatga atctcagcgg tgaggccgtc ctggccgcgg
     1981 ttaattttta taagatttca cctgatcgca tcgtggtcct gcacgatgag ctcgatatcg
     2041 actttggaca gatccgcctg cgggttggcg gctctagtgc cgggcacaac ggaatcaagt
     2101 cggtaacaca agcccttggc gaagactacg gccggatcag aataggggtc ggtcccaaga
     2161 agccggaccg aatcaagagc gaagactttg tccttcaaaa attttctagc gatgaacagg
     2221 cacagctacc gaacctctta aaagaagtta gcgccgtcct cagcgaattc atctacggcg
     2281 gccaattgtc acacgaaacc cgcaacttct tagtttaatc attgtgttcg ttggtaatcg
     2341 gtacatcgat aaggtagggc gctaattgtt ctactatccc ttttatcacc agctctttat
     2401 cttctgcaat gtccagtgct tttgatagtc tgtctccact cgtggcttcg tgatgatttt
     2461 cgccatacat aacctcaagc aaaccctcac ccgagcgcac gtctacatct aaatcatgcg
     2521 gagcggctaa ttcttcttcg cgagccttta tccctccttg tatcagaaaa ggattgtttc
     2581 gaagtatgtc aagtgaagtg gggttgggca ggccagttac gcgttcttgc tcttcggtaa
     2641 ggtagactaa cccaccctca atacggagtt tttccattgt gcttattata aaaagatgcc
     2701 cttaggcgga gagtaagata atttaccttt ccagataggg tttgagttct cccaatagtt
     2761 gagcttgtac ttgttcagaa tccgcctcca ctaaagctcg agaggacaaa gttggctcca
     2821 gaacatgagt tagctgcagg caagttctac ttacggccgc ctgcaaaccc cttaattcgg
     2881 ttcggcgtcc cctataacac gctcgagcat cggcgcctat tgcttcaatc tcgtctcgga
     2941 ctccgccaag tctgctggct acggcagcca gagctacttg ggtttcagcc tccggcattt
     3001 caggtttaag ctccggtaca ttaaaatact ctgtttcgct aaaaattaat cggccgtctt
     3061 cgtggtccat attctatcta taatgcccgc gctaggtttt ttaaaaaata aagaatttaa
     3121 caatcctcat gggtagagcg agtaaaggcg tttcggtggc gccgcatgct ttgacgattg
     3181 gtaaacgtat cgcccgatag ggccgcgcag cctgcaaagc gcgctggatc atgggtaagg
     3241 cgcggacttc gtggtgcagc gacatattcc tgcaagcttc ccaactccac aatgaggttt
     3301 tctacaattt ccaaatccgc ttcaggacca ttcccatcta ccaaggcttc tcttgtgttg
     3361 agcgcatcgt cgagcaggtc atctacgtca taaagaggga aggctttgtc ttgaccgatg
     3421 cccagaaggt atcgttcaag tttcgtaaaa taaagtcgcg aattgctagg gtcaaagtcc
     3481 ataaagataa ttgtatgtga ggtattgtat caaatttcac taaacctaaa gagcctccgg
     3541 agggggtggg caccgccgga ggctcattat acccttcaac ccaccccggc gaaacatcga
     3601 tgtctaaaca taggtgactc gtgtctccac ccctaagggg cagaaccgtt taggctcgct
     3661 ttgaatcggg gaccccggga aaacatccga agacgtgatc gaggaatcta gttaaagggg
     3721 ttagtatgtt ttagagcgtg tcccgcggga gcgagacaag cacaagagaa agtggagggt
     3781 aacaactttt catggcgcgc tctaaaacaa tctaactttt cctctagcca aacatgccag
     3841 aggtgttaga atgggggtaa aaggtgctta gaatccgcta cgcggaccca cgatttgcta
     3901 ttttagcaaa agcatgacgt tatgtcaata ctttaaaaca ttttcacatg attatcaaca
     3961 agcttacggt taatacagtc tcgattccac cggtgttaca gaagctttca agcccaccga
     4021 agcagctttt tcatgccggt tcaccgctcg ccccgttgct ggagcgtccc cgggtagcca
     4081 ttgtcggcag ccgcaacatc tcaccctatg gacgcgaagt gacttacagg ctggcgcggc
     4141 agctggcgga tcagggaatc gtcatcgtca gcggactagc cttcggcgtt gacaacatcg
     4201 cgcatcaggc gacactcgaa gccagtgga
//
LOCUS       DATNGP010000074         3055 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_3897936, whole genome shotgun sequence.
ACCESSION   DATNGP010000074 DATNGP010000000
VERSION     DATNGP010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3055
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3897936"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..680)
                     /locus_tag="VN778_01815"
     CDS             complement(<1..680)
                     /locus_tag="VN778_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008578413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HXR49742.1"
                     /translation="MNQLGRDAAAERIAKLRALINDYRYHYHVLDESTMSEAAADSLK
                     HELSELEAKYPDLITPDSPTQRVAGQPLPGFTQVRHHMRMISLNDVFNRDEVVVWIER
                     MDKLAPGRTREFFADIKMDGFACALLYEDGKFVQAVTRGDGYVGEDVTANVRTIDSVP
                     FELRAVKGFEQFLKGRTEVRGEIVIFKQDFAALNKQREVEGLPAFANPRNLAAGSIRQ
                     LDPKLAATR"
     gene            complement(677..1660)
                     /locus_tag="VN778_01820"
     CDS             complement(677..1660)
                     /locus_tag="VN778_01820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49743.1"
                     /translation="MAQGKSTKKPVVKQRVNKVAVPAEVIASKPRRLKLPAYKPFRLK
                     RVKYPEKLPSVWRLSAQTAQLVARRPWLFAGIAVVYAVLTLILVRGFGSGIDVASLKT
                     QLDTGPSHVAQLLTGVSVLVAMLNSSGTSAGDTSGAYQIIVGLVVSLATIWAARQTLA
                     GQSVGLRDAFYKGIYPLIPFVLVLLVVILESLPFLIGAWLYTTLVMNGIVATTLEQVI
                     WGIVCFVLVVISLYMLCSSLFALYIVTLPDMRPIKALRSARQLVRYRRGQVFRKLLYL
                     PVALLVITAVVMVPAILLVAGAATWVFFVWTTLILLIVHVYMYTLYKALLA"
     gene            complement(2225..>3055)
                     /locus_tag="VN778_01825"
     CDS             complement(2225..>3055)
                     /locus_tag="VN778_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HXR49744.1"
                     /translation="LFVAFVALIAWLFNQYLGGQPSTFYGVVIGTLIYAVITYFAGGR
                     MALAVNGAQEIQKKDNPRLWRIIENLSITDGLPMPRVFVINDPAPNAFATGRDPKHAA
                     VCATTGLLEIMDDNELMGVFAHELGHVKNYDIRVSMIAFALTAVISLFADIMLRLTWF
                     RGNDREDNNNGVFMLLGIVAALLAPLVALMIQLAISRQREYLADATSALTTRYPEGLI
                     SALRKIQHTGSVMQRQNTATAHLFFANPLRAHSLTALFSTHPPVADRIARLEHAEDHA
                     "
BASE COUNT          740 a          840 c          810 g          665 t
ORIGIN      
        1 cgggtggcgg caagtttggg gtcgagctgg cggatactgc cggctgccag gttgcgcggg
       61 ttggcgaacg ccggcaaacc ttcgacttcc cgttgtttgt tgagcgctgc gaaatcttgt
      121 ttgaaaataa cgatttcgcc gcggacctcg gttcggcctt ttagaaattg ttcgaagcct
      181 ttcaccgctc gcagctcgaa tggcacgctg tcgatggtcc gaacattggc ggtcacgtcc
      241 tcgccgacat atccatcacc acgcgttacg gcttggacga atttcccatc ctcgtatagc
      301 aaagcgcagg caaagccgtc cattttaata tccgcgaaaa actcgcgtgt tcgtcccggt
      361 gccagtttgt ccatgcgctc tatccagacc acaacttcgt cgcggttgaa cacatcattc
      421 aggctaatca tccgcatgtg gtgccgaact tgggtgaagc caggcagtgg ctgaccggca
      481 acccgttggg ttggactgtc aggagtaatg agatccgggt acttggcctc tagctcgctc
      541 agctcgtgct tcaacgaatc ggccgcggct tcgctcatgg tcgattcatc gagcacatgg
      601 tagtggtaac ggtaatcgtt gatcagggcg cggagtttgg cgatgcgctc tgccgcagca
      661 tcgcggccga gctggttcat gccagcaatg ccttatacag ggtatacata tagacgtgga
      721 caattaggag tatgagcgtt gtccacacga agaataccca ggtcgcggct ccggctacaa
      781 gcaagattgc cggcaccatt acgacggccg taataacgag cagcgccaca ggcaagtaca
      841 acaactttcg aaatacctga ccccgacggt agcgcactaa ctgccgtgca ctacgaaggg
      901 ctttgatagg ccgcatgtcg ggaagtgtca cgatgtacag cgcaaacaat gacgagcata
      961 gcatgtagag tgaaatgacc accaacacga aacagacaat accccaaatc acttgttcga
     1021 gtgtcgtcgc gacaatgccg ttcatgacca gcgtcgtata tagccatgca ccgattagga
     1081 acggtagcga ttctaggata acgaccagta gtacaagtac gaacggaata agcgggtata
     1141 ttcctttgta aaaagcgtct cggagaccga cgctttggcc tgccagtgtc tgccgtgccg
     1201 cccaaatggt ggccaagctc acgaccaggc cgacaataat ttgataggct ccgctcgtat
     1261 cacccgcgga tgtacctgac gaattaagca tggcaaccag aaccgaaact cctgtcaaga
     1321 gctgtgcgac atggctgggt ccggtatcaa gctgagtttt tagactagcg acatcaatcc
     1381 ccgatccaaa gccacggact aaaatgagtg ttagcaccgc atacactacg gcaatgccgg
     1441 cgaacagcca cggccggcgt gccacaagtt gagctgtctg agcgcttagc cgccatacgc
     1501 tcggaagctt ctctgggtat ttgacgcgct ttaaccgaaa gggtttatat gctggcagct
     1561 tcaggcgccg cggcttgctg gcgataacct cggcaggcac agccactttg tttacgcgct
     1621 gtttcacgac cggtttttta gtagacttcc cctgagccat agttgcacta atagtaccat
     1681 tttgaaccgg gttacgcacc caacaagcgt tcttttcaaa gaaccaaagt ctcatccttt
     1741 tggcattgcc caaaaggatg caaaaggcta ggccacttcg ttcgcgtggg caagcgttgt
     1801 aaagcccaac aaccggtact caaattcgcc ttcggctgat ttcgcaccgc tcccttcggg
     1861 agaagttgtt ggttttacta acaccttcgc catactcact ccgccacggc cataaacaac
     1921 caaggcggtt actcggctcc tcgccgagca aagctttaat tattaatcaa tagcggcagg
     1981 aaccgctcca tggctgtttt ctggccatgc ggaacattcc tggcgagacg ttgtataaac
     2041 gcacaatcta aactagccga ggaccgacga agaaaggcaa agtgaaacga gcctttctga
     2101 ggagtcccgc aggcgattgt gcggctttac aacggttccc aagtatgtga agctcggcct
     2161 cgagccttgg ctacctttgc atcaaggcaa aggtagagat tcgggttcct tggaaaggaa
     2221 cagattacgc gtggtcttcc gcgtgctcca gccgggcaat gcggtcggca accggtggat
     2281 gcgtactaaa gagcgccgtc aggctatggg cgcgtaatgg attggcaaaa aacaagtgtg
     2341 ccgtagcggt gttttggcgc tgcatcacgc taccagtatg ctggatttta cgaagcgcgc
     2401 tgattaaccc ttcggggtag cgcgtcgtca gggcactcgt agcgtcagcc agatattcgc
     2461 gctgccgcga aattgccaac tggatcatca gcgcaaccag cggcgccagc aatgcggcaa
     2521 caataccaag cagcataaaa acaccgttat tattgtcttc ccggtcgttg ccgcggaacc
     2581 acgtcaagcg aagcatgata tcggcgaaca gcgagatgac agccgtcagc gcgaaagcaa
     2641 tcattgatac gcggatatcg taattcttaa catgccccag ctcgtgcgcg aatacaccca
     2701 tgagctcatt gtcgtccatg atctccagta gccccgttgt cgcgcagact gctgcgtgct
     2761 tcggatcgcg gccagttgcg aaagcgttcg gcgccggatc gtttatcacg aatacgcgcg
     2821 gcataggcaa accatcggtg atgctcagat tctcgataat tcgccagagc ctcggattat
     2881 ccttcttttg gatttcctga gcaccgttaa cagccaaagc catacgcccg ccggcgaaat
     2941 aagtaattac cgcatatata agcgtgccga tgaccacgcc gtagaacgtc gacggctgcc
     3001 cgccgagata ttggttgaac agccaggcaa tcagggccac aaacgcgaca aacag
//
LOCUS       DATNGP010000075         3052 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_3605520, whole genome shotgun sequence.
ACCESSION   DATNGP010000075 DATNGP010000000
VERSION     DATNGP010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3052
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3605520"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..572)
                     /locus_tag="VN778_01830"
     CDS             complement(<1..572)
                     /locus_tag="VN778_01830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49745.1"
                     /translation="MKSLCIIYIPGLGNGDYLAQRKIVHWWHFLGVETELFPINWNDQ
                     KPWSSKRDALLVRVDALAAQGKRVALVGISAGGSAAINIYAARKNVITGIVCIAGWIN
                     YPEDINPIHLKINPAFVASAGQTAASLETLKGTYSRRILSLYALSDRIVNRRHSIIPG
                     ACNRSVLSIGHGFTIATQIVLGAPGFIRFL"
     gene            complement(602..686)
                     /locus_tag="VN778_01835"
     tRNA            complement(602..686)
                     /locus_tag="VN778_01835"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(650..652),aa:Leu,seq:tag)
     gene            complement(700..1230)
                     /locus_tag="VN778_01840"
     CDS             complement(700..1230)
                     /locus_tag="VN778_01840"
                     /inference="COORDINATES: protein motif:HMM:NF015559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HXR49746.1"
                     /translation="MRIIAGRLGGRNFSSPRGYHTHPMSDKVRGALFNALGDISGLTV
                     LDAFAGSGALSFEAISRGTTHALAIDANKSAQTAISENIKSLHLSSRVKLVKASASAW
                     LSTTNETFDIVLCDPPYDDLQTELLQRLADRTKRNGLVVFSLPPNANFVLSSSFQLLA
                     TKFYGDAQLVFYRRIA"
     gene            complement(1234..1983)
                     /locus_tag="VN778_01845"
     CDS             complement(1234..1983)
                     /locus_tag="VN778_01845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49747.1"
                     /translation="MARKNLERLMPQSHFPGTQAILHFEGKNGPDGIKRKSPAQDEPW
                     HYLQPFDPDDTQLIKLIENHYHDLVNALRHNDSVRASFEAAWLSHAIVDGLTPAHHFP
                     YEEKLIELSDGRSIDDRTTLSKKLIMPGPTTRHQVKNNWKMWGPKGLFTTHAAFECGI
                     AMLIAPIKIKRFLLTPDKIAEFNAQSLAQWFRQLAQDVARLELYDSFYESGWTVPLAR
                     RVRQNLAPTLVQAVALVWYNAALEAGVTIKA"
     gene            complement(2056..>3052)
                     /locus_tag="VN778_01850"
     CDS             complement(2056..>3052)
                     /locus_tag="VN778_01850"
                     /inference="COORDINATES: protein motif:HMM:NF012493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HXR49748.1"
                     /translation="EKRQAHQAIQDGKAGFIIGTQALIQEKVDMHKLAMVVVDEQHRF
                     GVEQRKALMAKAGHMPHVLSLTATPIPRSLALTLYGELDVSVLDEKPPGRQPIVTEII
                     SPNSRTQLNEKIDRELTIGHQIFVVCPVITDSETLEVNSAEAVYERLSSHDFKHRRVG
                     LLHGKLKPADKQAVMEQFVAHKLDILIATTVIEVGVDVPNATIMLIESAERFGLAQLH
                     QLRGRIGRGGHQAYCYLLLSDSRPPTRRLRALESSQDGFRLAELDLQLRGPGAIYGTM
                     QHGQLDLRIAKLTDTQLIAAARADAQRLIDEEENLLKYKELMKHVSRLRAVTNLN"
BASE COUNT          708 a          774 c          804 g          766 t
ORIGIN      
        1 tttaagaaac gaatgaagcc aggagcgccc aaaacaatct gcgtggcaat ggtgaagccg
       61 tggccaatac tcagcacact acgattacaa gctccgggga taatactgtg ccgtctgtta
      121 acgattctat cggataaagc atagagcgaa agtatccggc ggctgtatgt gccctttaat
      181 gtttccaggg atgcggcagt ttgcccggct gatgctacaa atgccggatt tattttaagg
      241 tgtatcggat taatatcttc gggataattg atccagcccg cgatacaaac gatacccgtt
      301 atcacatttt tacgggctgc gtagatattt atagctgccg agccgccagc gctaataccc
      361 accagtgcga cccgtttgcc ttgggcggcc agagcgtcga cccttaccag caaggcgtcg
      421 cgctttgaag accacggctt ttgatcgttc caatttatcg ggaatagctc agtttcaaca
      481 cctagaaaat gccaccaatg cactattttg cgctgggcta ggtaatcacc gttacctaga
      541 ccgggaatat atatgataca gagtgatttc acggtagtta ttttctgccc ccaaaaactg
      601 atggtgcgcg agagaggact tgaacctcca cgccttgcgg cactagctcc taaggctagc
      661 gtgtctacca attccaccac tcgcgcgtca ccgaatattt tacgcaattc ttcggtaaaa
      721 caccagttgg gcgtcgccgt agaatttggt agctagcaat tgaaaactgg agctgagaac
      781 gaaattggca tttggtggga gagaaaagac taccaaacca tttcgctttg ttctgtcggc
      841 caaacgctgg aggagctcgg tttgcaggtc gtcataaggc ggatcacata agacaatgtc
      901 gaatgtttca ttggtggttg agagccacgc actagcggaa gccttgacaa gtttgacccg
      961 actagacaga tgaagtgatt tgatattttc gctgattgcc gtttgggctg acttattcgc
     1021 atctatcgct agggcatgcg tcgtaccgcg actgatggct tcgaagctta aggcgccgct
     1081 gccggcaaag gcatcaagta cagtgaggcc gctgatatct cccagcgcat tgaagagagc
     1141 gccccgcact ttgtcgctca tggggtgcgt atgataccca cggggactac tgaagttgcg
     1201 tcctcctaag cggccggcaa tgattcgcac agcttaggcc ttaattgtta caccggcttc
     1261 tagggctgcg ttgtaccaga ctaaggctac agcttgcacc agagtggggg ccaggttctg
     1321 ccgcactcgg cgggccagtg gaacggtcca gccggattcg tagaacgagt cgtacaactc
     1381 taggcgagcc acgtcttgtg ccagttgccg aaaccactga gcaagcgact gggcgttaaa
     1441 ttcagcaatt ttatcagggg taagtaggaa gcgcttgatt tttataggag cgattaacat
     1501 ggcgatgccg cactcaaagg cggcgtgtgt cgtaaacagg cccttcggac cccacatttt
     1561 ccagttattc tttacctgat gccgggttgt cggaccaggc atgatcagtt ttttggaaag
     1621 cgtggtgcgg tcatcaatac ttcgcccatc gctgagctct atcaattttt cttcgtatgg
     1681 aaagtggtga gcgggcgtca ggccatccac gatagcgtgc gacagccacg cggcctcaaa
     1741 agaagctcgg acgctgtcgt tatgccttaa cgcgttgaca agatcgtgat aatgattttc
     1801 gatcaatttg ataagttgcg tatcgtcggg gtcaaagggc tgcaagtaat gccatggctc
     1861 atcctgggcc gggcttttac gcttaattcc atcggggccg tttttgccct cgaagtgcaa
     1921 aatcgcctgg gtgccaggaa aatgactctg gggcatcaag cgttcgagat ttttgcgtgc
     1981 caaacgatca atgttttggt gagccccgag gattcgtcca gaaaatgtgg aaatagttga
     2041 accagaatac attgcttagt tgagattcgt gacggccctc agccgcgata catgtttcat
     2101 taactcttta tattttagca ggttttcttc ctcgtcaata agtctctgcg catctgcacg
     2161 cgctgccgca atcagctgcg tatcggtaag tttggcgatg cgtaggtcaa gttgcccgtg
     2221 ttgcatggtg ccataaatag cccccggacc acgtagctgc aagtcgagct cggccagccg
     2281 aaaaccgtct tggctgcttt ccagagcgcg aagccgcctc gtcggtggcc gagaatcgct
     2341 aagtaacagg tagcaatagg cttgatgccc gccgcgacca atccgtcccc gcagctgatg
     2401 aagctgcgct aagccaaagc gttcggcact ttcaatcaac ataatcgtag cattgggcac
     2461 gtccacgccc acctctataa cggtcgtagc aatcaagata tccagcttgt gagccacgaa
     2521 ctgttccata accgcctgtt tatcggccgg ctttaattta ccgtgcagca ggccgacccg
     2581 gcgatgcttg aaatcgtgcg agctgagccg ctcgtacacc gcttctgccg agttcacttc
     2641 tagcgtttca gagtcagtaa tcactgggca aacaacgaat atttgatgcc ctatggttag
     2701 ttcacggtcg attttttcat ttagttgcgt tcgagagtta ggtgaaatta tttctgtaac
     2761 tattggttgt ctgccaggcg gtttttcatc gagcactgac acatccagct cgccatacaa
     2821 ggtcaaagcc agcgagcgcg gaataggcgt ggccgtcagg ctcaggacat ggggcatatg
     2881 accggccttg gccatgagag ctttacgctg ctcgacgcca aagcgatgct gctcgtcaac
     2941 gacgaccatg gccagcttat gcatgtctac tttctcttgt atcagcgcct gggtgccgat
     3001 aatgaagcct gctttgccgt cctgaatggc ttggtgggcc tgccgctttt cc
//
LOCUS       DATNGP010000076         3709 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_4236338, whole genome shotgun sequence.
ACCESSION   DATNGP010000076 DATNGP010000000
VERSION     DATNGP010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3709
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4236338"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..351)
                     /locus_tag="VN778_01855"
     CDS             complement(<1..351)
                     /locus_tag="VN778_01855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49749.1"
                     /translation="MEKEPKRPLYVDDIRAAAENSDAIQRDIIEQAEALRQEIAANQG
                     ASYEDVHAFMYKVGIFVLKKLQEKGIDDALELEGRLEELDNLHPNRSRADMFTVASYY
                     ETTSQESGIGYEEQL"
     gene            complement(384..623)
                     /locus_tag="VN778_01860"
     CDS             complement(384..623)
                     /locus_tag="VN778_01860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49750.1"
                     /translation="MTKESGDQELQELFDYDSLTEETLAYYGATEKAPDEAGQLSLEA
                     LYAETAEYRSRNLEHTIDLSFIDDVLPASPPPDRS"
     gene            complement(663..3473)
                     /gene="uvrA"
                     /locus_tag="VN778_01865"
     CDS             complement(663..3473)
                     /gene="uvrA"
                     /locus_tag="VN778_01865"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HXR49751.1"
                     /translation="MADEIVVKGAREHNLQNVDVTIPRNQLVVITGLSGSGKSSLAFD
                     TIYAEGQRRYVESLSSYARQFLGLMEKPDVDQIDGLSPAISIDQKSTSRNPRSTVATV
                     TEIYDYLRLLFARIGVPHCPIDGNPVVRQTTTGIVDQIVATTNGKRLMVLAPIVIDKK
                     GAFEHIPEQYQRAGFARARVDSVVYALDEFPQLDKAYRHRIEIVIDRLVNDEDSRSRL
                     VQSVEQALEVAEGKVVILDANNDETWTYSLMYACPDHPEITIPELEPRTFSFNSPHGA
                     CPVCTGLGSRLEVDPELVIPNGRLTIAEGAIRPYNRINVDNWYMKKLQAVADRYSFSL
                     HVPTSDLTSEQLNMIMYGTGSEKFRVSLGMGRSFETTYEGVVPNLERRHKETDSDFIR
                     RDIERFMQEKPCHACHGLRLKPEVLAITVGEQSIMDVCSLSIDDALQWFNSLQLDARE
                     AKIAQLILKEIGARLQFLQDVGLNYLNLLRSANTLSGGEAQRIRLATQIGSGLMGVLY
                     VLDEPSIGLHQRDNARLIATLKHLRDLGNTVIVVEHDEETIRTADFLLDIGPGAGIHG
                     GKIVAAGSPEAVAKVKDSVTAQYLAGAKEIATPKKRRPGNEKSLLIKGARENNLGNID
                     VEIPLGKLVVVSGVSGSGKSSLINDILAKELLARLHRASTVPGRHDDIEGIKQLDKAI
                     IIDQSPIGRTPRSNPATYTGLFTPIRELFAQMPEAKLRGYSAGRFSFNVKGGRCENCA
                     GDGIIKIEMHFLPDVYVPCEVCHGQRYNREALEIHYKGKTIADVLAMTCEQALEFFQN
                     IPAIARKLQTLVDVGLGYIALGQSATTLSGGEAQRIKLSTELSRRPTGRTLYILDEPT
                     TGLHMADVDKLLHMLHALVDAGNSMVVIEHNLDVIKNADWIIDMGPEGGNAGGQVVAE
                     GTPEQVAKAKNSYTGQYLKHVL"
BASE COUNT          826 a         1006 c          940 g          937 t
ORIGIN      
        1 taactgttcc tcataaccaa tgcccgattc ttggctggta gtttcgtagt agctggctac
       61 cgtaaacata tctgctctcg agcgattggg gtgaaggtta tcgagctcct ctagtctccc
      121 ctctagctcc aaagcatcat cgatgccttt ttcttgtagt tttttcagca caaatatccc
      181 gactttatac ataaatgcat gcacgtcttc atagctcgcc ccttgattgg cggcaatttc
      241 ttggcgcaag gcttcagcct gctcgataat atcgcgctgg atggcatcag aattttctgc
      301 tgccgcacga atatcatcaa catatagtgg tcgtttaggc tctttttcca tgctcatatt
      361 atagcatttt tgacaacggc tcgtcagctc cggtcggggg gtggtgaggc gggaagaaca
      421 tcgtcaataa aagacaagtc aatcgtatgt tctagattac gcgagcgata ctcggctgtt
      481 tcggcataca gtgcttccag gctgagctgg cctgcttcat cgggtgcttt ttctgtagcg
      541 ccataataag caagcgtctc ttcagtgaga gagtcgtaat caaataattc ctgtagttcc
      601 tggtcgcccg attcttttgt catacctcta tcctaagtga tgggttttta taaatctgtg
      661 aactaaagta catgctttag gtattggccg gtgtagctgt tcttggcttt ggctacttgc
      721 tccggagtgc cttcggcgac cacctggcca ccagcgttac cgccttcggg acccatgtcg
      781 atgatccagt cggcattttt aatgacgtcg aggttgtgct cgataacgac catgctgttg
      841 ccggcgtcaa cgagggcatg cagcatgtgg agcaacttgt cgacgtctgc catgtgcagc
      901 ccagtagtcg gctcatccaa aatatacagt gtgcggccag tcggacgacg gctgagctcg
      961 gtgctgagtt tgatacgctg ggcttcgcca ccactcaaag tggtcgccga ttggccaagg
     1021 gcgatgtaac ccaggccgac atcaacgagt gtttgcagtt tgcgggcaat agccgggatg
     1081 ttttggaaaa attccagggc ttgctcacag gtcatagcta gtacgtcggc aatggtcttg
     1141 cctttgtaat gaatttcgag ggcttcgcgg ttatagcgct ggccgtgaca gacctcacat
     1201 ggcacataga catcgggcag aaagtgcatt tcgatcttga tgatgccgtc gcccgcgcaa
     1261 ttttcacagc ggccgccttt gacattaaaa ctaaaacggc cggcagagta gccacgcaat
     1321 tttgcttcgg gcatttgggc gaataattct ctgattggcg tgaacaaccc agtgtaagta
     1381 gccgggttag agcgcggggt acggccaatc ggcgactggt cgatgatgat ggctttgtcg
     1441 agctgcttaa tgccctcgat gtcatcgtgc cggcctggca cagtgctagc ccggtgcaaa
     1501 cgcgccagta attctttagc gagaatgtcg ttaatcaagc tcgatttgcc ggaaccgctg
     1561 acgccgctga cgaccaccag tttgccgagt gggatttcga catcaatatt gcccaagtta
     1621 ttttcacgcg cgcctttgat cagcagggac ttttcatttc cgggccgtcg ctttttcggg
     1681 gtagcaattt ctttagcgcc tgccagatat tgcgcggtaa cgctatcctt gactttggcc
     1741 accgcctccg gtgaaccggc ggccacaatt ttaccgccat ggatgccagc cccagggcca
     1801 atgtctaaca gaaagtccgc tgtccggatt gtttcttcgt cgtgctcgac cacaatcacg
     1861 gtgtttccga ggtcccgcag gtgctttagg gtagcaatca gccgggcgtt atcgcgctga
     1921 tgtaaaccaa tactcggctc gtcgagtaca tacagcacac ccatcaagcc cgaaccgatt
     1981 tgggtagcca gccggatgcg ctgtgcctcc ccaccactga gtgtattagc gcttcttaac
     2041 aagttaagat agttcagccc cacatcttgc aggaattgca gccgggcgcc aatttctttt
     2101 aggatcagct gggcgatttt ggcttctctg gcatccagtt gtaggctatt gaaccattgc
     2161 agcgcgtcgt cgatcgataa gctgcagaca tccatgatcg attgctcacc gaccgttata
     2221 gctaagacct ccggttttaa ccgcaagcca tggcaagcgt ggcagggctt ttcctgcata
     2281 aaacgctcga tgtcgcgccg gataaaatcg ctatcagttt ctttatgccg gcgttccagg
     2341 ttcggcacca ccccttcata cgtcgtctcg aaggagcggc ccatacctag actcactcgg
     2401 aacttctcac tgccggtgcc atacatgatc atattgagct gctcggacgt taagtcactc
     2461 gtcggtacgt gcagactaaa actataccgg tcggcgacgg cttgcaattt cttcatatac
     2521 cagttatcga cattgatgcg gttatacggc cggatagcgc cctcggcaat cgttaaacgg
     2581 ccgttaggaa tcactagttc aggatcaacc tccagccggc tgcccagtcc ggtgcagacc
     2641 ggacaagccc cgtgcgggct gttgaagctg aaagtccgcg gctccagctc gggaatggta
     2701 atttctggat ggtccggaca ggcgtacatc aggctgtacg tccacgtctc atcattatta
     2761 gcatccagaa tgacgacttt gccttcggcg acctccaggg cttgctccac cgactgcacc
     2821 aggcggctac ggctgtcttc gtcgttcacc aagcgatcga taacaatttc gatgcggtgg
     2881 cgataagcct tatcaagctg cggaaattca tccagagcgt agaccacgct atcgacgcgg
     2941 gcacgggcaa agccggcccg ttgatactgc tcaggaatat gctcaaaagc acctttttta
     3001 tcgatcacga ttggcgccag aaccatcagg cgcttgccgt tagtggtagc cacgatctgg
     3061 tcgacgatgc cggtagtcgt ctgccgtacg accggattgc cgtcaatcgg acaatgcggc
     3121 acgccaatgc gcgcaaaaag caagcgcaaa taatcataaa tttccgtgac ggtcgccaca
     3181 gttgaacgcg gattgcgcga ggtcgatttc tgatcgatcg aaatagccgg acttaagcca
     3241 tcgatctgat cgacatcagg cttttccatc aagcccaaaa attgccgggc gtacgagctc
     3301 agtgactcga catagcgccg ctgcccttcg gcataaatcg tatcaaaggc caggcttgac
     3361 ttccccgacc cgctcaaacc cgtaatcacg accagttggt tccgcggaat cgttacgtcc
     3421 acgttctgca gattatgctc ccgcgcgccc ttgacaacta tctcatcagc cacctagttc
     3481 tccccttatt ttcccgtaaa actctcaatg taagccgata aattatacag gaaagtcaag
     3541 aaaaagtcga gcccctcagg cggctaagct ttctgcttga agcgcttcca tttcgagaat
     3601 aaccgcccgt ggtataacag caatttcggg ggcggcaata ggctgagcct ctaatccgta
     3661 attctctggg taataacacc aaggttctag cggcaaacct tcgttaagg
//
LOCUS       DATNGP010000077         5691 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_2473204, whole genome shotgun sequence.
ACCESSION   DATNGP010000077 DATNGP010000000
VERSION     DATNGP010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5691
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2473204"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..852)
                     /locus_tag="VN778_01870"
     CDS             complement(<1..852)
                     /locus_tag="VN778_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010164238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HXR49752.1"
                     /translation="MDKVLEHKLAELPKSPGVYFHKDASGEIIYVGKAAVLRNRVRQY
                     FQKSRTRDPKTEALVAEIVDTDWMVVDSELEALFLEAEMIRRYMPRYNILAKDDKALS
                     YIRIDYDSDYPTVTTTRRPLDDGARYFGPYFSTGAVRQSLKLLRRAFPFATRRVPGQK
                     RATLYYHLGLDPGLEEGRTSLEEYRANLRKLIAVIEGRRKTIVKEVERDMKRAAKGAE
                     FERAAKLRNQLFALQGLDRQVIFSDKEFLDISKDHALNELVNLLSMPNYPYRIEGYDI
                     SHMQGTDV"
     gene            complement(878..1756)
                     /locus_tag="VN778_01875"
     CDS             complement(878..1756)
                     /locus_tag="VN778_01875"
                     /inference="COORDINATES: protein motif:HMM:NF001134.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HXR49753.1"
                     /translation="MQKIILYYKFASITDPEAVRLWQRVLCEKLNLRGRILIADHGIN
                     GTLGGDLKDLKQYAKETKSFSPFNDIVFKWSEGGREGFPQLSVKVRPEIVAFGAKDEI
                     EVDASGIVGGGKHLKPKQVHDLVAQRGDDVVFFDGRNAYEASVGKFKNALVPDVQHTR
                     NFIEELENPKYDELKDKPIVTYCTGGIRCEVLSMLMKKRGFQEVYQIDGGIHTYGEEY
                     GDNGLWEGSLYVFDGRLTTKFSENAADIGACAHCNNATSRFDNCSNQTCNRHITVCEA
                     CLNKALCPDCYARVQA"
     gene            complement(1781..3370)
                     /locus_tag="VN778_01880"
     CDS             complement(1781..3370)
                     /locus_tag="VN778_01880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49754.1"
                     /translation="MEEFSLISKEETDSDKPESDKKKKKRAEALGALLVETKPEAQES
                     KTPREKLSTDEITLVRRVSAEAKLEALQEASDASESPETAYGDLLVEDFFEQIAHEAI
                     EPEEALQQTLAEAGIEELGPESEQSDTALEDEPLFDEAPREFGERDTEIYLHETPPPR
                     IDRRAEEAEPDGDGGVPPTPPIRPTARGRGDDQPPRRSRRSLYDAVPGAFDAGLHRAL
                     PDRLRTEYLSNYNEAGLLDAALVGGSGGIVGYLMGRRQGRIKSEKKLLPTQKRLEKQV
                     KDLQSELQIHETKLRKLAAQRVRARGPAVIERFKEAARSRPPRVEAVKLTPSPQHAPE
                     HLGHMVVVAEAKPVIKRQEARRTEKLVQRQQKPGETVAIVDKNIETMNHAELLELSDK
                     IVVDGASLRQIYESRLVGERGLRRLVSEQLHGGNVKKLLRQEILEREIDFERDPAMRD
                     QAAQSAEGAGGASLKDMLDRAGAAKTGSEEEAAFYKARAAYDSQQRAAQHQQRRLIDV
                     SMAATIAVLIALILILLLTRQ"
     gene            complement(3617..4105)
                     /locus_tag="VN778_01885"
     CDS             complement(3617..4105)
                     /locus_tag="VN778_01885"
                     /inference="COORDINATES: protein motif:HMM:NF021425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2127 domain-containing protein"
                     /protein_id="HXR49755.1"
                     /translation="MTLFHPQTLLDKTYEIGIIIKGVDGVLELIGGILVLALSPSAIT
                     GITQFLTERELEQDPHDFIATHILRAGQHLANGHNVFVAAFLLTHGLVKVVLVTCLLL
                     NKLWAYPWALGVLGLFLVYQIYQLITRPTFGMAFLTVLDAIIIWLVYREWQKVQPDAS
                     SD"
     gene            complement(4144..4860)
                     /locus_tag="VN778_01890"
     CDS             complement(4144..4860)
                     /locus_tag="VN778_01890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HXR49756.1"
                     /translation="MAQDAINLTLEPREVTGKAVKHLRNSGHVPAVIHDHGKDSVVVQ
                     AEYVKLLKVWQQAGKHHPVQLKTRDQTYTALIKSATFDPKKHQLTHVVFNAVDRNQTV
                     EAEIPVRPRYDEGNESAPAERNGLIVLAQLDTVQVKAIPTKLPDFLEYNAEKLVEVGD
                     HVTVADLIVPEDVEIETEAEHSIATVYEPSAIAAANEAAAGTAEAEPEAAEAKGETTG
                     EEGAAPEQEPAATEQESSNQ"
     gene            4954..5565
                     /locus_tag="VN778_01895"
     CDS             4954..5565
                     /locus_tag="VN778_01895"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HXR49757.1"
                     /translation="MFNVEHIIQAGGLALIALIIFGESGMFIGFFFPGDTLLLTAGIF
                     AGQGKLPLGLTIVVIALAAILGDNTGYHIGKRYGRRLFRKPDGLVFRQEYVQQAEKFY
                     ERFGSRTMLLAHFVPVVRTFAPVVAGIARMNYAKFALFDAIGDSAWAVIVTLIGYWFG
                     TKIPNIDHYILLAVAGVMIFTLGPTLYHLGKAILEKRRTTPSD"
BASE COUNT         1239 a         1520 c         1391 g         1541 t
ORIGIN      
        1 cacatcagta ccttgcatat gactgatgtc atagccttca attctatagg ggtaattagg
       61 catacttaac aagttaacta attcatttaa agcgtggtct ttactgatgt cgagaaactc
      121 tttgtcactg aaaatgactt gccgatccag gccttgcaag gcaaaaagct gattgcgaag
      181 cttggcggcc cgctcaaact ctgccccttt agccgctcgc ttcatgtccc gctccacctc
      241 tttgacaatt gtcttgcgcc ggccttcaat gacagctatg agcttcctga gatttgcccg
      301 atattcttcc agacttgtgc gcccttcttc cagaccggga tcaagcccga gatggtagta
      361 taaggttgct cttttctgtc cggggacccg acgagtagcg aacggaaaag ctcggcgcag
      421 tagtttcagg gattgccgca ctgcaccagt actaaagtac ggcccgaagt agcgcgcacc
      481 gtcatccaag ggccgccgcg ttgtcgtgac tgtcggataa tcgctgtcat aatcaatgcg
      541 aatatagctc aatgccttgt catccttagc gaggatgttg tagcgcggca tatagcggcg
      601 gatcatttcg gcctcaagga ataaggcttc aagctcgcta tcgacaacca tccagtcggt
      661 gtcgacaatc tcagctacca gggcttcggt tttaggatca cgcgtccgag atttctgaaa
      721 atactggcgt acccggttcc tgagcaccgc ggctttgccg acatagataa tctcgccgct
      781 agcgtcctta tgaaaataaa cgcccggtga tttcggcaac tcagctagtt tatgctccaa
      841 aactttatcc atagcttgta tatattgtaa cgcttcttta ggcttggacg cgcgcgtagc
      901 aatcgggaca aagcgctttg ttcaagcagg cttcacaaac cgtgatgtgt cgattgcatg
      961 tttggttgga acaattgtcg aacctgctgg tggcgttgtt gcaatgagca cacgccccta
     1021 tgtccgcggc attttcggaa aacttagtcg tcaggcggcc atcgaaaacg tacagcgaac
     1081 cttcccagag gccgttatcg ccgtactcct cgccatacgt atgaatgccg ccgtcgattt
     1141 ggtacacctc ttggaagcca cgctttttca tgagcatgct gaggacttcg cagcggatac
     1201 cgccggtaca gtacgtcaca atcggcttat ccttgagctc gtcatattta ggattttcca
     1261 attcttcgat gaaatttcgg gtgtgctgca catcgggtac gagggcattt ttgaatttcc
     1321 cgacagacgc ttcgtaagcg ttccgaccat caaagaaaac cacgtcatca ccgcgctgag
     1381 ccaccaaatc gtggacctgt ttcggcttca ggtgcttccc gccgccaact atgccgctag
     1441 catcaacttc gatctcatct ttggcgccaa aagcaacaat ttccggacgg actttcacgg
     1501 atagttgagg aaagccttcc cgcccgccct cactccattt aaaaacaatg tcgttgaatg
     1561 gactaaaact ctttgtctct ttagcatact gttttaaatc ttttaagtcg ccgccgagcg
     1621 tgccgttgat accatgatct gcgatcaaaa tcctacctct gaggttcagt ttttcacaca
     1681 gtacacgttg ccacagtcga accgcttccg ggtcggttat tgacgcaaat ttgtagtaca
     1741 aaatgatttt ttgcataagt cgttagcctc tattataccg tcactggcga gtcagcagga
     1801 gtatgagaat cagcgctatc aagacggcga tggtcgccgc catggagacg tcaattaggc
     1861 ggcgttgttg gtgttgcgcc gcgcgctgtt gcgagtcata agcagccctg gctttataaa
     1921 acgcagcctc ctcttcgctg ccggttttgg cggctccggc gcggtcgagc atatctttga
     1981 gtgatgctcc gccagcgcct tccgcgctct gagcagcttg gtcgcgcatg gccgggtcac
     2041 gttcgaagtc tatttcgcgt tcaagaattt cctgacgcag cagttttttg acattaccac
     2101 catgcaattg ctcgctgacg agacgccgta agcctcgctc accaaccaaa cgcgattcgt
     2161 aaatttgacg caagctggcg ccgtcaacaa caatcttatc gcttaactcg agtagttcgg
     2221 cgtggttcat ggtttcaata tttttatcaa ctatggccac agtttcgccg ggcttttgct
     2281 gccgttgaac taatttttcc gtccgccttg cttcttggcg cttgatgacc ggcttcgctt
     2341 cggcgaccac gaccatatgt ccgagatgtt ccggcgcgtg ctgcgggctt ggcgtcagct
     2401 tgactgcttc aactctggga ggacgtgagc gtgccgcctc cttaaatcgt tcaatcacgg
     2461 cagggccgcg agcacgaaca cgctgagccg ccagtttccg aagtttcgtt tcatggattt
     2521 gcaactcgct ctgtaaatct ttgacctgtt tctcgagtcg cttctgagtc ggcagcagtt
     2581 ttttctccga cttaatacga ccctggcgcc gccccatcaa gtagccgacg ataccgccgc
     2641 tacctcccac taaggcagca tcaagcaagc ctgcttcgtt gtaattcgaa aggtattcag
     2701 tacgtaaacg atcgggtagt gcccgatgca gccctgcgtc aaacgcgcct ggcacagcgt
     2761 catagagcga tcttcgcgaa cggcgcggcg gctgatcgtc acctctgccg cgagccgtgg
     2821 gccgaattgg tggggtgggc ggcacaccgc cgtctccatc aggttcggct tcttcggcgc
     2881 gccgatcaat gcgcggtggc ggggtttcgt gaaggtaaat ttcagtgtca cgctcaccaa
     2941 attcacgcgg cgcctcgtca aacaatggtt cgtcctccag agctgtgtcc gattgctcag
     3001 actccggccc caattcttcg atgcctgctt cagccaacgt ctgctgcagc gcctcttccg
     3061 gctcaatcgc ttcatgagcg atctgttcga aaaaatcttc gacaagcaaa tcaccgtaag
     3121 ctgtttctgg tgactcgctg gcatcagatg cttcttgtaa ggcctctagc ttggcttccg
     3181 ctgatacacg tcgcaccaga gttatttcat cggtgctcag tttttcgcgt ggagttttgg
     3241 attcttgagc ttccggcttg gtttcaacca ataaagcgcc gagtgcttcg gcacgtttct
     3301 ttttcttctt gtccgattct ggcttatccg agtcagtttc ttcttttgaa attaacgaga
     3361 attcttccat gagatgatgc ctgcagcgac aaacacttta gtataggatg agatacgcta
     3421 tttagcaagg ttatctccta catgatcagc tggtacagtt gtaatcaatt ccttcttata
     3481 cttgccttga cacgcgccct tataaaagtg gtatgaaagc catatgaaaa catatgtttt
     3541 catgcagcct tcataatgac tatgtaatgg ggctattttg cgtgtactga tgtgagcact
     3601 ttattgagaa taaacttcag tcagaactgg catcaggctg gactttttgc cattcgcggt
     3661 aaactagcca gataataatc gcgtctagaa cagtcagaaa ggccatcccg aaagttggtc
     3721 gagtgatgag ctgataaatt tggtatacca ggaacagccc cagtacaccg agcgcccatg
     3781 gataggccca taacttattg agcagtaaac aagttaccaa aacaactttg accagtccgt
     3841 gggttagcaa aaaggccgcc acgaagacat tgtggccatt agccaggtgt tggccggccc
     3901 gcagaatgtg ggtggcgatg aaatcatgcg gatcttgttc tagttcccgc tctgttaaaa
     3961 attgtgtgat gccagtaatc gcactcgggc ttaaggcgag caccagaata ccgccaatga
     4021 gctccagcac accgtcgaca cccttgataa tgataccgat ctcataggtt ttatcgagca
     4081 gtgtttgcgg gtgaaataag gtcatagacc tactatagca tcgctcgaag caccggttct
     4141 ggattattgg ttagacgact cttgttcggt ggctgctggt tcctgctcgg gtgccgcgcc
     4201 ctcttcgccg gtagtttcgc ctttggcttc agctgcttct ggctcggctt ccgcagtgcc
     4261 agccgcggcc tcgttggcag cagcgatggc actcggttcg taaacagtag caatggagtg
     4321 ttcggcttcg gtttcgatct ccacatcttc gggcacgatc agatcggcca cagtaacgtg
     4381 atcgccgact tcgaccaact tttcagcatt atactctaag aagtctggta atttggtcgg
     4441 aatggctttg acttgcaccg tatcaagctg ggctagcacg atcaggccat tgcgctcagc
     4501 gggagccgat tcgttgcctt cgtcatagcg cgggcggact ggtatttcag cttcgaccgt
     4561 ttgattacgg tcgacggcat tgaaaacgac atgtgttagt tgatgctttt taggatcaaa
     4621 ggtggcggat ttaatcaatg cagtgtaggt ttgatcgcgg gtttttagct gtaccgggtg
     4681 gtgcttacct gcctgttgcc agactttgag cagcttgacg tattcggcct gcacgaccac
     4741 tgagtctttg ccatgatcat gaatcacggc aggcacatgt ccgctattgc gtaaatgttt
     4801 gacggccttg ccggttactt cacggggttc tagcgtcaaa ttaatagcat cctgcgccat
     4861 tgattattac ctcctctgtt tcttgtaatt aattataaca gatgttttag gagagctgga
     4921 cgctccccat cgaaaccggt aagctaagag ggcatgttta acgtcgagca tattattcaa
     4981 gccggtggct tagccctcat cgctctcatt attttcggcg aatccggcat gtttatcggt
     5041 tttttcttcc ctggcgacac actcctactg acggccggca tttttgccgg acagggcaag
     5101 ttgccgctcg gactaacgat cgtcgttatt gccctcgccg ccatacttgg tgacaacact
     5161 ggttatcaca tcggcaaacg gtatggccga cgcctttttc gtaaacctga cggcctggtt
     5221 ttccgccaag agtatgtgca gcaagccgag aagttttatg agcgctttgg cagccgcacc
     5281 atgctgctgg ctcattttgt gccggtcgtc cgcacctttg ccccggtcgt cgccggtatt
     5341 gcccgcatga attatgccaa gttcgctctc ttcgatgcta tcggcgacag cgcctgggct
     5401 gtcatcgtca cgctaattgg ttattggttc ggcactaaaa tccccaatat tgaccactat
     5461 attttgctag cggttgccgg cgtaatgatc ttcaccctcg gccccactct ttaccacctc
     5521 ggcaaagcca ttctcgaaaa gcgccggact actccaagcg actaatcatc gtgtaatccc
     5581 gcacgcaact caacaagtaa gccgattaat gcatcgtgag tgtagtctgg tatatcaaac
     5641 ccaacccctt taattgtctc ttccgcctcg taaaaccttt ccatggtgtt a
//
LOCUS       DATNGP010000078         6568 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_3511950, whole genome shotgun sequence.
ACCESSION   DATNGP010000078 DATNGP010000000
VERSION     DATNGP010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6568
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3511950"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1964
                     /locus_tag="VN778_01900"
     CDS             <1..1964
                     /locus_tag="VN778_01900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49758.1"
                     /translation="VPGADDGTWGDILNAYLSVTHASDGTLNSNVVGTSQILNNAVTN
                     AQLDSATQTILSSVAGKYTLPAGGIPESDLDSATQTKIDVGASAYQKPVGGIPGSDLD
                     SSVQSDLASASTAVQSVNSKAGTSVTLSASDISAVPASQLGAASGVATLNASSQLTAS
                     QLPSIAGNASAATFTNASVLGASKRPAVDLLMSSYDTVASSTDQTAAFQSAISAAANI
                     LSSSSVIKVRIILDSRQKYIIGGAVQSGANGQLAQIPLPYSSGAVAGTIELVGFPGAG
                     GPEWLILGYGQSGTVIESTLASAPTFSASTGIASTFGGPASYSTNNKNAQSTICFSVK
                     NVEIRSAAPVICGIDTGHLAGFFFDGLIFNTDATTNMNGSSGALAAYTTMVTNLVVTT
                     APQAIPIIFPLVNCVYGAKGGMLGVANWACGPVIGEWLDAQQIDVMFCPGPCLAVDVS
                     SHPNRIGMLGDWDNAYGISSTYPTSGNNPFAPTTTGGASDTTAGSNTASMLDIGVWDV
                     QTAESYAPNTIKRVNDVLDSNGIAPINAMAQKVQGGTGAILTKQWSFIGTGASAAGIM
                     YYSKIIDKMSAIGAIQTASGFPKTSGTTARNPISRDCLVYTSGGTITNYSINGSAFGA
                     APPSSFLLPANATFSITYTGTPAMSFMAI"
     gene            complement(2112..3074)
                     /locus_tag="VN778_01905"
     CDS             complement(2112..3074)
                     /locus_tag="VN778_01905"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF016362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HXR49759.1"
                     /translation="MTAKKSAKPNKPKWKDFEESARQASSKIFSNQTVKPNQKVMGSI
                     SQVLRQVDVKVGDEDFIDIECKDHKKPVDLPKVEQFANKLKDENAKSGIMISNAGFTK
                     SALRTAEHYNIKPVVLVDKGHADTSNFMIKAPVLAEGFYLDSISYGLEQTGQDFRFSP
                     LVWDMHVSDGSDPPRSIYELFRDHWNDGTIDITRVGSYQYRFEQVPIIDLRGDVIIVD
                     LTYRYIVKADRRKGSAAAQIARGFYDLNNKSFTSSEEIVFEPIEIEKISDWPELSPDE
                     DPPQQPFRLVGLFPLPDEPPLSSYTNPFTNEPLSPDKVKQHIYI"
     gene            complement(3071..3979)
                     /locus_tag="VN778_01910"
     CDS             complement(3071..3979)
                     /locus_tag="VN778_01910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49760.1"
                     /translation="MIWAAHFPGLFYNCEHASYDLTPFPDSIIQAGKVFASNRQIIPT
                     EDHNGTVAIALELSSNPNESTYYKSIERLKNLVCLSYTSLQQEQRWENPGESLDEVVT
                     SNLAERFYNPDVRYGEQLDWDLPLRIAAYSQNIAGLSPENQTKFWQALQTFCYARQIA
                     QLPNPQYKYTLYMTLHLSSINQLASEPQSLHTPAVHLVCPECNETLGGLTHSTSHLAE
                     IKNLIDRLVPSEHAQRNKNFIDNLFHRIRSSFIHRGEFAGSEDVGGFIALFDSNVELL
                     ENDINLKVLNRSLLEQFIVQNQEERL"
     gene            complement(3989..4576)
                     /locus_tag="VN778_01915"
     CDS             complement(3989..4576)
                     /locus_tag="VN778_01915"
                     /inference="COORDINATES: protein motif:HMM:NF013492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HXR49761.1"
                     /translation="MSQNEALINSLELAAWAARETPIRFFGDVVLRTVSTPVEEHEFG
                     GVEVTRIADELTATLIKYREKTGLGRGLAANQIGYTRRIIAVLFDDEPQVMCNPKVVS
                     SEGLGSYWESCISSGALLVGEVHRPWTATFSYNAVDGSEQHIEADEKQTRVLLHEIDH
                     LDGITCDEKYEAKTMKVVTGGKEEIFGYAFSKIEQ"
     gene            complement(4573..5142)
                     /locus_tag="VN778_01920"
     CDS             complement(4573..5142)
                     /locus_tag="VN778_01920"
                     /inference="COORDINATES: protein motif:HMM:NF024635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXR49762.1"
                     /translation="MTVEQIVKAITQKPSNHKPVLIGIEGFGGSGKTTFASRLRDSLG
                     SAYVVNIDDFIVKEKLTEPSWDKGGFDRERLEQQVLLPASKQEPVQYQELIWETNTLS
                     EPKVVPPVDYLIIEGISCYHPNIQHYYDYKIWVDTPIEVAKARGKERDAGNENADKWD
                     MWAENDLAYQEKYHPELKADFIVSGEDKQ"
     gene            5643..5852
                     /locus_tag="VN778_01925"
     CDS             5643..5852
                     /locus_tag="VN778_01925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49763.1"
                     /translation="MSGQNDQLVISVKEARKLLGLKSKNLTNEELKLLIQDTQTVVRI
                     CVREYIGSKNSENNATIPSTKATTL"
     gene            5849..>6568
                     /locus_tag="VN778_01930"
     CDS             5849..>6568
                     /locus_tag="VN778_01930"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HXR49764.1"
                     /translation="MKHSVIYLRVSTTKQVEGASLETQQMLCQEWNTRNQIMVERTFH
                     DDGVSAKTLDRPAMKEMLSYLEANKGKISYVVTYQTDRLTRNATDFFALRAKLSQMGI
                     EYKNINSSLEDSVNEELIQNIEAVLAQHDNKVKSVRVKDNMKAHAKQGYRMSKAPHGL
                     KNVRDVLDKSVLAPIDGVADKVAAVLEAHATGTHTMSGLIKMCESIGLKSAHGKPMQI
                     QAISKMLRNPIYAGLEQSSHTD"
BASE COUNT         1779 a         1557 c         1434 g         1798 t
ORIGIN      
        1 cagtacccgg agcagacgat ggcacctggg gagatatcct caatgcctat ttgagcgtga
       61 cacacgccag tgacggtacg ctcaacagta acgtggttgg caccagccaa atccttaaca
      121 atgccgttac caatgcccag ctagatagcg ctacccaaac gattctatcc tcagtcgccg
      181 gcaaatatac actaccagct ggtggcatac cagaatcaga cttagatagt gctactcaaa
      241 ctaaaataga cgttggtgcc agcgcctacc aaaagccagt tggcggcatt cctggctcag
      301 atctggacag tagtgtacag agcgatctag catcggcttc tacggccgtc caatccgtca
      361 atagtaaagc gggtaccagc gtgacgttga gtgccagtga tataagcgct gttcctgctt
      421 ctcaattagg agcagccagt ggagtagcca cgctcaacgc gagttcgcag ctgaccgcca
      481 gccagttgcc gtctatagct ggaaacgcga gcgctgcaac ttttacgaat gcttccgtcc
      541 taggagcgtc aaagcgtccg gcagtcgatc ttctcatgtc gtcctatgac accgtcgcct
      601 catcaactga tcagacagcg gcattccaaa gtgctatttc agccgcagcc aacatcctat
      661 cttctagcag tgttatcaaa gtccgaatta ttctagatag tcgccaaaaa tatataatag
      721 gcggagcagt tcaatcaggc gctaatggcc agctggctca gatcccgctg ccatactcca
      781 gcggagctgt tgccggaaca atcgagttag tcggttttcc tggcgccggt ggcccagagt
      841 ggctcatact cggctatgga caatctggca ccgtcattga gtcaaccctc gcgagcgcgc
      901 ctactttttc ggcttcgacc ggtatcgcat cgacttttgg tggccctgcc agctactcaa
      961 cgaacaataa gaacgcgcaa tcaacgatct gcttttctgt caaaaatgtg gagatccgtt
     1021 ccgccgcccc ggtaatttgc ggcattgaca ccgggcatct ggccgggttc ttttttgacg
     1081 ggctcatttt taatactgac gccacgacca atatgaatgg tagtagcggc gcacttgccg
     1141 cttacacgac aatggtcacc aatctcgttg tcacaacagc accgcaagcc attccaatca
     1201 tttttcctct ggttaattgc gtttacgggg ccaagggtgg aatgctcggt gtcgctaatt
     1261 gggcctgcgg gccggtaatt ggagagtggc ttgatgctca acaaattgat gttatgttct
     1321 gccccggtcc ttgcttggct gtcgacgttt caagtcaccc taaccgaatc ggcatgctgg
     1381 gcgattggga caatgcctac ggcatttcat ctacctatcc cacttcaggc aataatcctt
     1441 tcgcaccaac gacgacgggt ggcgcaagcg acacgacggc tggaagcaat actgcttcaa
     1501 tgctagacat tggtgtctgg gacgtgcaaa ctgccgagag ttatgcccct aacaccatca
     1561 aacgcgttaa tgacgtgctc gactccaacg gtattgcccc aatcaacgcc atggcgcaaa
     1621 aggtacaggg cggtacaggt gctattttaa ctaaacaatg gtcttttata ggcaccggtg
     1681 cgagtgccgc gggtataatg tactactcaa agattattga taaaatgtct gctatcgggg
     1741 ccattcaaac ggcgtctggc ttcccaaaga cgtcaggcac gacagccaga aacccaattt
     1801 cacgcgactg cctagtatat acatccggtg gcaccataac aaattatagt attaatggat
     1861 cggcatttgg cgcggcgcca ccaagctcct ttttgctgcc agctaacgct acgttttcta
     1921 taacctacac cggcacgccc gcaatgagct tcatggcaat ctgaacaaaa agcagacttc
     1981 taattaatta cagctaattg tggtgaacta cctgaaatcg aaatggaacg aaatctgtag
     2041 cgaactactc gaagtataca actatatgag acaaataaca gtgatggtta atattcccgc
     2101 ttagagtaga actatatgta tatatgttgt tttactttat caggacttaa tggctcatta
     2161 gtaaacgggt tagtgtaact agagagtggt ggctcgtctg ggaggggaaa taagcccacc
     2221 aatcgaaatg gctgttgagg tgggtcctca tctggtgaaa gctctggcca atcactaatc
     2281 ttttcaattt ctatcggttc aaaaactatt tcttccgatg aagtgaagga cttattatta
     2341 aggtcataaa acccacgagc aatttgtgca gcagcagaac ccttccttct atcggcttta
     2401 acgatatatc ggtaagtcag gtctacgatt attacatcac cacgcaagtc aattatcgga
     2461 acttgctcaa agcgatactg atacgaaccc acccgtgtaa tgtctatggt gccgtcattc
     2521 caatggtccc taaataactc gtaaatactc cttggtgggt cagaaccgtc agacacgtgc
     2581 atgtcccaaa caagtggcga aaatctaaag tcctgccctg tctgttcaag accgtaggaa
     2641 attgaatcaa ggtaaaatcc ttccgcaagc acaggtgctt taatcataaa attactcgtg
     2701 tctgcatgac ctttatctac caaaaccact ggcttgatgt tgtaatgctc ggcagtcctt
     2761 aaggcagact tggtaaaacc cgcatttgaa atcattattc cacttttagc attttcgtct
     2821 ttcagcttat tcgcaaactg ctcaacctta ggcaaatcaa ctggcttttt gtggtcttta
     2881 cattcgatat ctatgaaatc ttcatcaccg accttaacgt caacctgtct tagcacctgg
     2941 cttatagacc ccataacttt ctgatttggt ttcacggttt ggttactaaa tatctttgag
     3001 ctagcttgcc gtgcgctttc ttcaaagtct ttccactttg gcttattggg cttggctgat
     3061 ttctttgctg tcataatctt tcttcctggt tctgtacaat aaattgctct agtaacgacc
     3121 tgttgagcac ttttaagtta atatcattct ctagcagttc aacattactg tcaaaaagtg
     3181 caataaaacc accaacatct tccgaaccag caaactcgcc ccgatgtatg aagcttgacc
     3241 taatacgatg aaacaagttg tctataaaat ttttattcct ttgagcgtgt tcggaaggta
     3301 cgagcctatc tattaggttc ttaatttcag ctaaatggct ggtgctatgc gttaagccgc
     3361 caagtgtttc attacattct ggacagacta gatgtacggc aggagtatgt aagctttgcg
     3421 gttcacttgc taactggtta attgaagata ggtgtagggt catatacaaa gtgtacttat
     3481 attgaggatt gggtagttgt gctatttgac gcgcatagca aaatgtttga agtgcttgcc
     3541 agaacttagt ttgattctct gggcttagtc ctgcgatatt ttgactatat gctgcgattc
     3601 gtaaaggcaa gtcccaatca agctgttcac catatcttac gtctggattg taaaaacgtt
     3661 ccgcaaggtt agaagtaact acttcgtcca atgattcacc aggattttcc cacctttgtt
     3721 cttgttgcaa ggaagtgtaa gacaagcaga cgagattttt tagccgttca atacttttat
     3781 aatatgttga ctcattggga tttgaactaa gttctaatgc aatcgcaacc gtgccattat
     3841 ggtcttctgt tggaatgatt tgcctgttac tggcaaaaac ttttcccgct tgaattattg
     3901 agtcggggaa tggcgttagg tcgtatgaag catgctcgca gttataaaaa agccctggaa
     3961 agtgtgcagc ccaaatcata attcatcttt actgctcaat tttactgaac gcatagccga
     4021 agatttcttc tttaccacca gtaacaactt tcattgtttt agcttcgtac ttctcgtcac
     4081 aagttatgcc atcaagatgg tcaatctcgt gaagtaaaac acgggtttgt ttttcgtccg
     4141 cctctatgtg ctgctccgat ccatcaacag cattgtagct aaatgttgcc gtccaaggtc
     4201 tgtgcacctc accaaccaac agtgctcccg acgatatgca gctctcccag taacttccga
     4261 gtccttcgct agacactact tttgggttgc acataacttg tggctcatca tcaaacagga
     4321 cagcgatgat ccgtcgcgtg tagccaattt ggttagctgc cagcccacgc cctaaacctg
     4381 ttttctcgcg gtactttatg agcgtagcgg ttagttcatc tgcaattcga gttacttcaa
     4441 caccaccgaa ctcatgctcc tcgacaggcg tgctcaccgt ccttagaaca acatcaccaa
     4501 agaaccttat aggtgtctca cgtgcagccc aagccgctag ttcaagacta tttattagtg
     4561 cttcgttctg actcattgtt tgtcctcgcc actaacaata aaatcggctt ttaattcggg
     4621 gtgatacttc tcctgatacg ccaagtcatt ttctgcccac atatcccact tatcagcatt
     4681 ctcattccca gcgtcacgtt ccttgccccg tgctttagct acttcaatag gagtatctac
     4741 ccagatttta tagtcatagt agtgctgtat attcgggtga tagcatgaaa tgccttcgat
     4801 gattaggtaa tcaacggggg gcactacttt tggctcactt aaggtattcg tttcccagat
     4861 aagctcctgg tactgtacag gctcttgctt tgacgctggc aacaatacct gctgctccaa
     4921 acgctcacgg tcaaagccac ctttatccca agatggttca gttagcttct ctttcacaat
     4981 aaaatcatct atgttcacga cgtaggcact gcccagtgaa tcacgtagcc tactggcaaa
     5041 cgttgttttg cctgagccac caaagccctc tatgccaata agcacaggct tatggttgga
     5101 tggtttttgc gtgattgctt taacaatctg ctccacagtc atagcagtat cataacaggg
     5161 aggaaacggt aaagccacag ggatggtatc ttctttggct tagacgctaa aacctctcca
     5221 aaggtctgga aatgcgtcac agggcgtact gtagctaaaa gctcctgttc atccagtttg
     5281 ataaattggc ggtatcatag aatacgtgct agtttttctt aaatactgat gtaatttaac
     5341 caacactagg cagtggggag agtgtacgcg aacaccgttg agattcacaa ttagcacaag
     5401 caggatgcct ctgtaaatgg tagttattac accataaatt gcttgcttgt cattaacttc
     5461 gcgaagtata attagaatag agttaagttg ggttctctca tagttaccac gctctcatgc
     5521 ccccatgaaa gtgcgacatc caacagactg gctagggacc aggccattag gggatgaaca
     5581 gcctaatcca atagcattct cgtaagttca gcgagataag cgattggatt atttttatgt
     5641 ctatgtctgg acagaatgac caactagtca tctcggtaaa agaagctcgt aagcttttag
     5701 ggctaaaatc aaaaaaccta accaacgaag aactgaaatt actcattcaa gatactcaaa
     5761 cagtggtgag aatttgtgtc cgcgaatata ttggttcgaa aaatagcgaa aataatgcta
     5821 ctattccaag taccaaggca actacactat gaaacattca gttatttatc ttcgcgtatc
     5881 aaccacaaaa caggtagagg gtgcaagcct cgaaacgcaa cagatgttgt gtcaggagtg
     5941 gaacactcgt aatcagatta tggtggagag gacgtttcat gacgacggtg tttctgctaa
     6001 gacgttagac cgtcctgcca tgaaagagat gctgagttac cttgaagcaa acaaaggcaa
     6061 gattagctat gtggttacat atcagactga ccgtttgaca aggaatgcaa ccgacttctt
     6121 tgccctgcgt gccaaattat ctcagatggg aattgagtac aaaaatataa actcaagcct
     6181 agaggacagc gtaaatgagg agcttataca gaatatagag gctgtgcttg ctcaacatga
     6241 caataaggta aagtctgtac gtgtgaaaga caacatgaaa gcccacgcga aacaaggtta
     6301 ccgcatgagc aaggcacctc atggattaaa gaatgtcaga gatgttctcg acaagtctgt
     6361 tctcgctccc atagatggag ttgctgacaa ggtggctgca gttcttgaag cacacgcaac
     6421 gggcacccac acaatgtctg gcttaatcaa gatgtgtgag agtattggac ttaagtcagc
     6481 acacggcaag ccaatgcaga tacaagcgat aagtaagatg ttgcgtaatc cgatttatgc
     6541 ggggcttgag cagagtagcc atacagac
//
LOCUS       DATNGP010000079         2598 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_3744399, whole genome shotgun sequence.
ACCESSION   DATNGP010000079 DATNGP010000000
VERSION     DATNGP010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2598
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3744399"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1629)
                     /locus_tag="VN778_01935"
     CDS             complement(<1..1629)
                     /locus_tag="VN778_01935"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXR49765.1"
                     /translation="MNLALFLFIRSFRSGWKRLSLIVGAVGTGVLVLLLFTTGYNGLI
                     GRSSHSSWRQAIFQAQDNGGAPQKPTPGVQPLYAILSSTSNIDTLWRNTQIYVTSLAA
                     SGASSPHLQDLPTPGPGEYYVSPGLKKVMDEHPEDNLGQRFGTRLLGIVPDKYVTSPD
                     ELSVIRGMILTQTKRLDVIGASGTKLYHIDENQAASVGYSNALMVVLYLGIFILLFPV
                     MLLISIATQLGSAQREQRYAALRLVGATKAQVRRFMMVESLIGSVTGIVVGFVLYVAT
                     QPLVLGFKFEDMRFWPSDMTLHMSEIVGIIVVTLLFTALANWWGMRHVQTSPLGVVRR
                     GSRRKNPRWWRLILLALGLGILGLAAIAPHKTSSEKSIVAQLILASVVVLMFGLVIAG
                     PYVTRRLSGFVARRTKRAETLLGMKYISLNSRGVFRSVAGVVVALFAGSFYLASTSGI
                     ESLSARSVDDNGYTQLRSSTMLVGTYATGAPLPTGQAGALQRLGYVQHVTEVKEVGNS
                     SILVMPCQVATQYTSIRCPTGSKYAGIDFSKQDLP"
     gene            complement(1626..2303)
                     /locus_tag="VN778_01940"
     CDS             complement(1626..2303)
                     /locus_tag="VN778_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013169656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXR49766.1"
                     /translation="MPVAPIISAKNIEKSFGATHALRRLSLDVEPGEVLAIMGPSGSG
                     KTTLLHSLAAITSIDNGEITLDGKRIDKLSDNARSRMRRQQFGFVFQFSQLVPELTAL
                     DNVALPLLLNGVKRHEAYKRAKQWLDEVGLEKQYDNLPGELSGGQTQRVAIARAMVAE
                     PMILFADEPTGSLDSLNSEKVMELFIDSAKKHGTTVIMVTHEPSIAAYADREIIVRDG
                     QISGAKK"
     gene            complement(2294..>2598)
                     /locus_tag="VN778_01945"
     CDS             complement(2294..>2598)
                     /locus_tag="VN778_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013169655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HXR49767.1"
                     /translation="GQHTLRVWLETPEVPSPQLQATLYMKTVLAIMENGDAAPYLDNQ
                     RKSHIQRMRSLTRARRDVPLATKLLIDHAIFHIEADLRWIELTSSRLTQLKEDLCL"
BASE COUNT          620 a          679 c          685 g          614 t
ORIGIN      
        1 aggcaaatct tgcttactaa aatcgatacc ggcgtattta ctaccggtgg gacaccgtat
       61 cgacgtatac tgcgttgcca cttggcacgg catgacgaga atggaagaat tacctacctc
      121 tttgacttca gtcacatgtt ggacgtatcc aagtctttgc aatgcgcctg cctgccctgt
      181 cggcagcgga gcgcctgtag cgtacgtgcc gaccaacatg gtgcttgagc ggagctgcgt
      241 gtagccatta tcatccactg accgtgcaga taagctttca ataccactag tgctcgccag
      301 ataaaaactt ccggcgaaga gcgctactac gacgccagca acactgcgga acacaccgcg
      361 agaattgagc gatatgtact tcatgccgag cagcgtttcg gcgcgtttgg tgcgccgtgc
      421 aacaaatccc gacagtctcc gcgtgacgta cggtccggcg atgacgaggc cgaacattag
      481 taccacgacg ctggccagga tcaactgcgc gacaatactt ttttcgctgg atgttttatg
      541 gggcgcgata gcagcaaggc ccaatatgcc tagccctagc gcaagtagaa tgagccgcca
      601 ccagcgcgga ttttttcgtc gtgaaccgcg gcgaacaacg ccaagcggtg aggtttggac
      661 gtggcgcata ccccaccaat ttgcaagtgc agtaaacagc agcgtaacga cgataatacc
      721 gactatttca ctcatatgta gcgtcatatc gctcggccaa aaacgcatat cctcgaactt
      781 aaacccgagt accagtggct gggtagcaac gtatagtacg aagcccacga caataccggt
      841 aaccgacccg atcaacgatt ctaccatcat aaagcgccgc acctgggctt tggtcgcacc
      901 gaccagtcgt agggcagcat agcgttgttc gcgctgggca ctaccaagct gcgtagcgat
      961 ggagatgagc agcatgactg gaaagagtag tatgaaaata ccgaggtaca gcaccaccat
     1021 taatgcgttg ctatagccaa cagaggcagc ttggttttca tcgatatgat agagcttggt
     1081 acctgatgca ccgattacat ccaggcgctt ggtctgtgtg aggatcatac cgcgaataac
     1141 cgacagctcg tcaggtgagg tgacgtattt gtccggaact atgccgagta gtctcgtacc
     1201 aaagcgctgc ccgagattat cctccgggtg ctcatccata accttcttga ggccaggcga
     1261 gacatagtat tcaccgggac ctggtgttgg caaatcttgc aaatgaggcg agcttgcacc
     1321 gctcgcggct aggcttgtga cgtaaatttg tgtgttgcgc cacaacgtat caatgttcga
     1381 agtcgatgaa agtattgcat agagcggctg cacacccggc gtgggcttct gcggggcgcc
     1441 tccattgtct tgagcttgaa aaatggcctg tcgccatgac gaatggctgc ttcggcctat
     1501 cagcccgtta tatccggttg taaagagtag cagtactaga acgccagtgc cgactgcgcc
     1561 cacaataagc gacagtcgtt tccagccgct gcgaaatgat cggataaaca aaaacagagc
     1621 aagattcatt ttttagctcc gctaatttgt ccgtcacgca caatgatctc acggtcggca
     1681 taggcagcaa tgctcggttc gtgcgtcacc attatgaccg tcgtgccatg cttctttgcc
     1741 gaatcgataa acagctccat gactttttcg gaattgagcg agtcgagaga accggtcggt
     1801 tcgtcggcaa agagaatcat cggttcagcg accattgcgc gagcgatagc aacgcgttgc
     1861 gtttggccac ctgacagttc gccgggtaga ttatcatatt gcttttccaa gcctacctcg
     1921 tcgagccatt gtttcgcgcg tttgtatgct tcgtgtcgct tgacgccgtt taggagcagc
     1981 ggcagcgcga cgttatcgag tgcagttagt tccggtacga gctggctaaa ttgaaagaca
     2041 aagccaaatt gttggcggcg catacgactc cgtgcattgt cgctgagttt atcaatacgt
     2101 ttgccgtcta gcgttatttc gccattgtcg atgctggtaa tggccgccaa actatgcagc
     2161 aaggtcgttt tgcccgaacc gctcggcccc ataatcgcca gaacttcccc aggctcaacg
     2221 tcgaggctca gcctacgcag cgcatgtgta gcaccaaatg atttttcaat attttttgca
     2281 ctaataatcg gtgctacagg catagatcct cctttagttg tgtcagcctt gagctggtaa
     2341 gttcaatcca gcgtagatcc gcttcgatgt gaaaaatcgc atggtcgata agcagcttgg
     2401 tggcgagcgg gacatcacga cgcgcccggg taagcgagcg catacgttgg atatgtgact
     2461 tacgctgatt gtcgagatat ggtgcagcgt cgccattttc cataatcgca agcaccgtct
     2521 tcatatagag tgtagcttgt agctgtggcg acgggacttc tggcgtttca agccacactc
     2581 gcaaggtatg ttggccag
//
LOCUS       DATNGP010000080         2171 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_1190664, whole genome shotgun sequence.
ACCESSION   DATNGP010000080 DATNGP010000000
VERSION     DATNGP010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2171
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1190664"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..567)
                     /locus_tag="VN778_01950"
     CDS             complement(<1..567)
                     /locus_tag="VN778_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009772503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXR49768.1"
                     /translation="MSTKPQLRLTLIIAILASFVAFLDGSVVNVALPSITRELGGGLV
                     TQQWVVDSYLITLGALMLIAGSLSDLYGRKQVLRWGLLGFGAASLLCAIAPTSTTLIV
                     ARGLQGVAGALLVPSSLALIISAFSGSAQGKAIGSWTGWTGIAFVIGPLLGGFLVDSA
                     SWRLIFAINVMPIAVTLWLLQNLSNRDER"
     gene            complement(580..990)
                     /locus_tag="VN778_01955"
     CDS             complement(580..990)
                     /locus_tag="VN778_01955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49769.1"
                     /translation="MDIQHAHVRHPREILQAKKRAKRENNLFMYYNDVVLEWITNHIL
                     ASVILFDAALIAPLLAIPGPDTIKITLGVISGSWIQLWALPALQRSQNKTQAQNDAKA
                     EVDHETLTYLATLQDEQMAELKGITSILDQLKKR"
     gene            complement(1073..1339)
                     /locus_tag="VN778_01960"
     CDS             complement(1073..1339)
                     /locus_tag="VN778_01960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49770.1"
                     /translation="MSKESPNQGSDTAKQPGHRDFGLFPGEDGQAHSTRPDQWDTTSP
                     SEMFDLGLPFTNTGLPSVLGAQSRAAPPKPDTKFDQQRYVSEAE"
     gene            1518..1889
                     /locus_tag="VN778_01965"
     CDS             1518..1889
                     /locus_tag="VN778_01965"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXR49771.1"
                     /translation="MLKLNSLLIGSEDPEQLAAFYGEVLQSKPGWQGGGYTGYQAGDA
                     YLMIGPHDKVHGRSQNPERIIFNFETADVRAEFDRIKAIEGSTIVREPYNPGDNESST
                     LLLATLADPDGNYFQLATPMQ"
     gene            complement(1895..>2171)
                     /locus_tag="VN778_01970"
     CDS             complement(1895..>2171)
                     /locus_tag="VN778_01970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49772.1"
                     /translation="KWWLTALQCPLRSFAVQDRVGCLRESLTEKGSRHRTEVYSTCLQ
                     LPGQRGCGLVSQLATARRNLYLRARAWADVCRLTTSPVIRDAQEDYE"
BASE COUNT          512 a          601 c          554 g          504 t
ORIGIN      
        1 tcgctcgtcc cggttactca ggttctgcag caaccacagc gttaccgcga tcggcatcac
       61 attgatagca aatatcagcc gccaggatgc actatctacc agaaaaccgc caagcagcgg
      121 gccaattaca aacgcgatac cggtccatcc ggtccagctg ccaattgcct taccttgggc
      181 cgatccggag aacgcggaga taatgagcgc cagtgagctt ggcactagca aggcgccggc
      241 aactccctgc agcccgcgcg ccacaatcaa tgttgtgctg gttggcgcta tcgcgcagag
      301 tagcgacgca gcaccaaaac caagcaaccc ccagcgcagg acttgtttgc gcccatagag
      361 atcagatagc gaaccggcaa tcagcatcag cgcaccaagc gtaattaagt atgagtcgac
      421 gacccactgc tgagtcacca ggccaccgcc tagctcgcgt gtgatagaag gcaaggctac
      481 atttaccacc gatccgtcaa gaaacgcgac aaacgacgct agaatggcaa tgatcaacgt
      541 gagtcgaagt tgtggcttcg tcgacatttt tgtattcccc tatctcttct tcagttggtc
      601 aagaatacta gtaatgccct tcagctcggc catttgctcg tcctggagtg tcgctagata
      661 tgtcaatgtt tcgtggtcga cctcagcttt ggcgtcattt tgagcctgag ttttgttctg
      721 gctgcgctgt agcgccggca atgcccacag ctggatccag ctaccggaga tcacacccag
      781 cgtaatttta atagtatccg ggccggggat cgcaagcagc ggcgcaatca gcgctgcgtc
      841 gaataaaatt actgacgcca agatgtggtt cgtgatccat tccagcacca catcgttgta
      901 gtacatgaac aaattatttt cgcgcttcgc acgttttttt gcctgtaaga tctctctcgg
      961 gtgccgaacg tgagcatgtt gtatatccat gcctgtacta tagcaccatg gacagagcag
     1021 aaaataagcc tagttattca ccaggcttat tattgtgcca gaaagctgta catcactcag
     1081 cttcgctcac gtaacgctgt tgatcaaact tcgtatctgg cttgggcggt gctgctcttg
     1141 attgggcgcc gagcacggac ggtaagcccg tattagtgaa tggaaggcca agatcaaaca
     1201 tttcacttgg tgatgttgta tcccattggt cgggacgcgt agagtgtgct tggccgtctt
     1261 cgcccgggaa aagcccgaaa tctcggtgac caggttgttt agctgtatct gaaccttgat
     1321 ttggagattc tttgctcatg gatgcaagta aagcataaag actatatctt gtccgtaccc
     1381 taatttgatg cacgtgctag ccgtcgccct ctcattttga ctatctaaaa cgtaggactg
     1441 gagagagatg tagcctttac caccaagcat tatggagcga tgcggtacaa tgggacgagt
     1501 aaacaaggag catcgcgatg ttgaagctta attcactgct aatcggtagc gaggaccccg
     1561 aacagttagc cgccttttac ggcgaagtgc tgcagtctaa gccgggctgg caaggcggcg
     1621 gttataccgg ctaccaagcc ggagacgcgt acttgatgat cggcccgcac gataaagtcc
     1681 acggcaggag ccagaatccg gagcgcatca ttttcaattt cgagacagcc gatgtccggg
     1741 ccgaatttga ccggatcaaa gccatcgaag gctctacgat tgttcgcgag ccttacaacc
     1801 ctggcgataa cgagagcagt acgttgcttc tagccacgct cgccgacccc gatggcaact
     1861 acttccagct cgccaccccc atgcaataga ctattcactc gtagtcttct tgagcatctc
     1921 taattactgg gctcgtcgtt aatcggcaga cgtccgccca agcgcgtgcc cttaggtaga
     1981 ggttccggcg tgccgttgcc aattggctca ccaggccaca tccccgttgc cccggaagtt
     2041 ggagacatgt tgaatacact tcggttcggt gcctgctccc cttctccgtc aatgactccc
     2101 gcaggcagcc aacccggtct tggacagcga aactcctgag ggggcattgg agtgccgtca
     2161 accaccactt g
//
LOCUS       DATNGP010000081         4775 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_4211644, whole genome shotgun sequence.
ACCESSION   DATNGP010000081 DATNGP010000000
VERSION     DATNGP010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4775
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4211644"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..861
                     /locus_tag="VN778_01975"
     CDS             <1..861
                     /locus_tag="VN778_01975"
                     /inference="COORDINATES: protein motif:HMM:NF019142.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HXR49773.1"
                     /translation="QTGKQIYPEDIVTAAPVDNFLAQQAIGSVVTIKRSLPVTLDING
                     FPAYTRTQATTVGAFLREKGIELGKTGTVNPAQATPLQAGQLISVVRDGTGIRSIVET
                     IAMPVQVIYDSSLAYGTSAVRQAGNPGRRVLTYRIVVKNGQVVSKELVQSVTTEDAVT
                     QIVVEGTNLSGIKGDMAQAGIPASQYQYADYIISHESGWCPTKWQGEVGYCPPTYQAI
                     HNTQGPDSNGLTYGFGLCQSTPAGKMASAGDDWAINPITQLKWCTGYADRRYGSWYNA
                     YSYWIIHHNW"
     gene            1019..1816
                     /gene="rsmA"
                     /locus_tag="VN778_01980"
     CDS             1019..1816
                     /gene="rsmA"
                     /locus_tag="VN778_01980"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010165636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HXR49774.1"
                     /translation="MELPFAKKSLGQHWLTDQITLVAMCDAAEIVADDTVLEIGPGLG
                     TLTTELVKRAKQVIAVEFDADLAREIPYRVPAGNLAIVQQDILRFDVTRLPAGYKVVA
                     NIPYYLTSNLLRVLSEALNPPAVAVLLVQKEVAERVVARPGDMSLLSVLAQFYWEVSL
                     GREVPAKLFTPPPKVDSQILILRRRKQPLFPDTDTKVFFRIVKAGFSQRRKTLLNSLS
                     AGLHLSRERTHEMLQASSVDPTVRAQSLSMEQWYVLYKSAGTHFTAD"
     gene            1969..2427
                     /locus_tag="VN778_01985"
     CDS             1969..2427
                     /locus_tag="VN778_01985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49775.1"
                     /translation="MFRTSSESNGAEQPVIVGSWTTPGLPVASFAIRSLSNYVVEEGT
                     GKYVRVLPGFDPQAASRVYRRGLEVTTTTYVEGDAGQDPYGGRQGVVVTDQSITDLYN
                     NPGRDIARHIGDFAAKVALWAMHDEAAETRWIVERILAQALGELIDPQTP"
     gene            2464..2667
                     /locus_tag="VN778_01990"
     CDS             2464..2667
                     /locus_tag="VN778_01990"
                     /inference="COORDINATES: protein motif:HMM:NF024804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HXR49776.1"
                     /translation="MSKTYDLMQLLDHVAATVAKQSDQVLQEQLGIGLSQFKILQVLQ
                     AEPRTPQREIARSLAQTEASISR"
     gene            2668..2925
                     /locus_tag="VN778_01995"
     CDS             2668..2925
                     /locus_tag="VN778_01995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49777.1"
                     /translation="MKLMLEQGLIHRLRGPDDHRQHLTVVTHKGERLAMAALIALARY
                     HRPTVSALTEKQQEQLVAALETLHRQLCVESHATPAADQPL"
     gene            3136..3524
                     /gene="ssrA"
                     /locus_tag="VN778_02000"
     tmRNA           3136..3524
                     /gene="ssrA"
                     /locus_tag="VN778_02000"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            3703..4167
                     /locus_tag="VN778_02005"
     CDS             3703..4167
                     /locus_tag="VN778_02005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49778.1"
                     /translation="MTQDIAKTDRITSGEYAQWSLEHDTEPGDEGAGWAPYNGILERA
                     LTEAGITPPELAEHALDALDDPRVREHAHELVEAGDADLEPVHRLEARSVRDRELFMA
                     RWLVSSNFLALTGLRFGKRHPDFQRELEIALLLNGVMPTDNEIRSNIIRVQQ"
     gene            4235..4519
                     /locus_tag="VN778_02010"
     CDS             4235..4519
                     /locus_tag="VN778_02010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49779.1"
                     /translation="MPLPPDEPDEEEKLEEEPNDFDTPFSLPAEREVDIADASGKTRR
                     RPDLESTHPATDSNLQDEELYDEGLSGAAEVSEPNPNDAVIAYHPRKKHK"
BASE COUNT         1199 a         1203 c         1275 g         1098 t
ORIGIN      
        1 cagacaggca aacagatcta ccctgaagac atcgtaacgg ccgcgccagt cgataatttt
       61 ctggcccagc aggcgatcgg aagtgttgta accatcaagc ggtcgctacc ggtgaccctc
      121 gacatcaatg gcttcccagc ctacacccgt acccaggcga cgacagtggg cgctttcctc
      181 cgtgaaaaag ggatcgagct cggcaagacc ggcaccgtga acccggctca ggccacaccc
      241 ctgcaggccg gccagctaat cagtgtggtg cgtgacggca ccggtatccg cagtattgtc
      301 gagacaatcg ccatgccggt ccaggttatt tatgactcat cgctggccta tggtacctcg
      361 gcagtgcgcc aagctggcaa cccgggacgc agagtcctga cctaccggat tgttgtcaaa
      421 aatggccaag tcgttagcaa ggagctcgtc caatcggtaa cgaccgaaga tgccgtgacg
      481 cagatcgtgg tcgaaggtac gaacttgtcg ggcatcaaag gcgacatggc gcaggccggc
      541 atccccgcca gccagtacca atacgccgac tacatcatta gtcatgaatc cggctggtgc
      601 ccaactaagt ggcagggtga agttggctat tgcccaccga cgtatcaagc cattcacaat
      661 acgcaaggtc ctgattccaa cggccttacc tacggctttg ggctctgtca gtccacccca
      721 gcggggaaaa tggccagtgc cggtgacgac tgggcaatca acccgatcac tcagctgaag
      781 tggtgtaccg gctacgcgga tcgccgctat ggcagctggt ataacgccta tagctactgg
      841 ataattcacc ataattggta gctacgctaa gagaggaaaa cccaagctaa agcttgaggg
      901 ttttcctctc ttagctcgcc ttcggctcgg atctttcctt tccgtcagcc agttataacg
      961 atgagtgaat caccgttata actgtattta tctgttaaac aaccgtaaaa tggccaatat
     1021 ggaattaccg tttgctaaga agtcgctggg gcaacactgg ttgaccgatc aaataaccct
     1081 agtggcgatg tgtgatgctg ctgaaatcgt cgccgacgac actgtgctag agattggccc
     1141 ggggctgggg acgctgacga ctgagctcgt aaagcgagcg aagcaggtta tcgctgttga
     1201 atttgacgct gatttagctc gggaaatacc gtatcgagtt cctgctggca atctggccat
     1261 tgtccagcaa gatattttgc gctttgatgt gacccggctg ccggctggat ataaagtcgt
     1321 cgccaacatt ccttattacc tgaccagcaa tttattacgc gtactgtcgg aggcgctcaa
     1381 cccgcccgcg gtagctgtcc tgcttgtcca gaaggaagtt gccgagcgcg ttgttgcccg
     1441 tcctggcgat atgtcattac tgagcgtact ggcgcaattt tattgggaag tgagcctagg
     1501 ccgtgaagtc ccggcgaaat tgttcacgcc gccaccaaaa gtcgactcac agatccttat
     1561 cctccgccga cgcaagcagc cgttattccc agatactgac accaaagtat ttttccgcat
     1621 cgtcaaggcc ggcttttccc agcgccgcaa aacacttctc aacagcctga gcgccggcct
     1681 gcacctcagc cgcgagcgta cgcacgaaat gcttcaagcg agcagtgtcg accctactgt
     1741 ccgtgcccaa agcctgtcga tggaacagtg gtatgtcctc tataaatcag ctggtacgca
     1801 ctttacagca gattgagttt aatgtagggt atatatcaca tgcgcaatcc ccctaatgta
     1861 ttagacgatc gattccatgc gatatttttt gattggaacg gctcgcgcag agaaccccgc
     1921 ggcaaccatc tctccctgcc aactggtggt tctgtaaact gggtcgcaat gttcagaacc
     1981 tcctcggaat caaacggagc ggaacagccg gttattgtag gttcatggac gacgccaggt
     2041 ttaccggtag cgagttttgc aatccgtagt ttaagtaact acgtagtaga agaaggcacc
     2101 ggtaagtatg tgagggtatt acctggcttt gatccacagg ctgcttcgcg ggtctatcgt
     2161 cgcgggttgg aggtgacgac aacaacctac gttgagggcg acgcgggaca ggatccatac
     2221 gggggtcgac agggtgttgt tgtgacagac cagtcgataa ctgatcttta caacaatcct
     2281 ggcagagata tcgcaaggca catcggagat ttcgcagcaa aggttgcttt gtgggcgatg
     2341 cacgacgagg ccgccgaaac gcgatggata gtagaacgta ttcttgctca agctctgggt
     2401 gagttaattg accctcaaac tccttgacgt gttaagtatt tttgctatcg tggttatata
     2461 accatgagca aaacctacga ccttatgcag cttctcgacc acgtcgcggc gacggtcgcc
     2521 aagcaatccg atcaggtgtt gcaggagcaa ctggggatcg gcttatcaca gttcaagatc
     2581 ctgcaggttt tgcaggcaga accgcgaacg ccacagcgtg aaattgctcg gagtctggcc
     2641 caaacggaag caagtatcag ccgttaggtg aagctaatgc ttgaacaagg tttgatacat
     2701 cgcttgcgcg gtccggacga ccaccgacaa catctgacgg tcgttaccca caaaggcgag
     2761 cgcttagcta tggctgcact gattgcgctg gcgcgttacc atcggcccac ggtatctgct
     2821 ctaaccgaaa aacagcaaga gcaactggtc gctgcgcttg agacccttca ccgacaactg
     2881 tgtgtcgaat cgcacgccac cccagcggca gatcaaccac tttaatattc acgaaataaa
     2941 gcaaaagtgg tataataaat tagccgataa cagattgttt tctgttgttg gggttccgca
     3001 gacaacgtct gcacacaatt tggccaagcc tgggagcgaa gtcgaccgct tggacgattg
     3061 agaggaagac cataggctag ggtcaaagct tttgccgtta tcggcaaaac gagactaagc
     3121 cgtaaggcct gacgaggggc tgacttaagc tcgacgttgg attgcaatgg ttagatccgc
     3181 aggtcgcttg ctaagcgtta tatggcaacc tgaatagatg caaaaaactc tattgcagac
     3241 gcaaaggcag ctgtagcgga cttgttcgct cgcagcaact ttgcaccagt tgcagcctag
     3301 ttgctgcagc aacgcctgac gcgatatcag atagtgccac agcgtttaat atcatctgat
     3361 tgctcggata atcttgtcca gattttccgc taagaccaag accgctcgcg tggaatgttt
     3421 gtcagtttcg atacccgcgc gctaagacca taaaattgac taagcctgta gtagactgac
     3481 taacgcaccg gcggaccgga gtgcaaatct ccgcagctcc accaatgcaa aaacctcact
     3541 ttggcgtgag gtttttgcat tagatcgatt ttttgggttc tgcactagtg aggtgggaac
     3601 tgccagtggc tattctagtc tttcatgctt agcccggtaa cgaaacattt gttagccgga
     3661 gcagctgcta taatcggcac taacaattaa gggagactta gcatgacgca agacatagca
     3721 aagactgacc ggataacgag tggtgagtat gcgcaatgga gtctagagca tgacactgag
     3781 ccaggcgatg aaggggcggg gtgggctcct tataacggca tacttgaacg tgcgttaact
     3841 gaggccggca taactcctcc agaactagcc gaacatgcgc tggatgctct cgatgacccg
     3901 agagtgcgtg agcatgcgca tgagctagtt gaggccggtg acgctgattt ggagcccgtc
     3961 catcgacttg aggcacgctc tgttcgcgat agggaactct ttatggccag atggcttgtg
     4021 tcgtctaatt ttctggctct tacaggtttg cgcttcggta agcgccatcc tgattttcaa
     4081 agggagctag aaattgcact gttgctaaac ggtgttatgc cgactgacaa tgagatcaga
     4141 agtaacatta ttcgtgtgca gcaatagtgg tcgcgagtta gttagctcac agagccgctc
     4201 gtaagagggt gctacggtag gtaaggaggt atatatgcca ttgccgcctg acgaaccgga
     4261 cgaagaggag aagctcgaag aagagccaaa tgactttgac acgccgttta gtctccctgc
     4321 tgaacgcgaa gtagatattg ccgacgcaag tggtaaaacc cgcagacgtc ctgatctcga
     4381 atccacgcac cccgcaactg acagcaatct gcaggatgaa gagctctacg acgagggtct
     4441 ctctggtgcc gccgaggtca gtgagcccaa cccgaacgac gccgtgatag catatcatcc
     4501 gcgaaaaaag cataaatagc tgaaggccgc caaatgcact gaccccgaat ggagtgaaac
     4561 tttaatagtt ctttgattta tggtatagta gacatacttt gaattaaaaa ggattatgaa
     4621 atgacagatg atgcgaaggg tgaattaatt ggtgtgcttc ctgatattga acgagcacaa
     4681 attattgctg atagattgag agatgctttt gcaagagacc ctcaaacggg tgatcttgct
     4741 gacgtgtttg gggtgatggt gaactttgcc cttag
//
LOCUS       DATNGP010000082         2742 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_1986728, whole genome shotgun sequence.
ACCESSION   DATNGP010000082 DATNGP010000000
VERSION     DATNGP010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2742
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1986728"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..386)
                     /locus_tag="VN778_02015"
     CDS             complement(<1..386)
                     /locus_tag="VN778_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HXR49780.1"
                     /translation="MTERQQKILNAIVEQYAEVASPVGSMLLAKLFHVSSATIRAEMA
                     ELERLGYITQPHTSAGRVPTDKGYRLYVNNINESEGASLPERRAEKALAQRVQNAGAP
                     ERMIRNAVDTLVELTHNLGMATIGNQ"
     gene            700..1350
                     /locus_tag="VN778_02020"
     CDS             700..1350
                     /locus_tag="VN778_02020"
                     /inference="COORDINATES: protein motif:HMM:NF012624.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HXR49781.1"
                     /translation="MHHSLNANQISKIKSWLGTSSLNIFGRPFAGKDTQGKILSDMFD
                     GQLLGGGDILRNSTIPERSQQALKQGLLIPTDEYLEIVLPYFSQDAFKDKPFILSSVG
                     RWHGEEPGVLEATERSGHPIKVVLYLDIPEEAARVRRHKSREERSRGDRGSRHDDAPE
                     IFETRLKEFRQKTLPVIEYYQATPLFAHIDANRPGVEVTAQILEELLARAEHKKPE"
     gene            complement(1357..2004)
                     /locus_tag="VN778_02025"
     CDS             complement(1357..2004)
                     /locus_tag="VN778_02025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49782.1"
                     /translation="MDETEKRLNQKLLIALFTPSWLSGLLAVSLGLVVIVGVIVLFNL
                     HNSQVQQQLVGLQSTAQQSALTLPGEAPPSAGTNSLQNTWPLLAFWGVIGFVVYFVIE
                     TVVKALSSIAELQDELHYVHAKREALLKTTIEYLLLRIVAAVVWLIFIDIFFKRIVPY
                     SITAAYASASSPLSLNSFLYALLSFAMIALSMHAHAIFLRLAVRRPRVFSASEYI"
     gene            complement(2056..>2742)
                     /locus_tag="VN778_02030"
     CDS             complement(2056..>2742)
                     /locus_tag="VN778_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641513.1"
                     /note="back-translocating Elongation Factor EF4; binds to
                     the ribosome on the universally-conserved alpha-sarcin
                     loop; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HXR49783.1"
                     /translation="PSTDYQVTLVSGEELDIKSAADLPDPAKINEIREPWIVGEIIVP
                     QEYVGPVIQLVSAKRGRQNNISYVDQRALITFEAPLANLLTDFYDQLKSLTSGYGSFN
                     YELADYRAEDLVRLDFYVAGEMVSALSLMVHRSEAQMLGRDIVQKLKDVIPRQNFQVA
                     LQAAIGGKFIAREDLSAYRKDVTTGLYGGDVSRKKKVLAKQAKGKKRLKRFGKVDIPA
                     EAFTVLLKRD"
BASE COUNT          673 a          705 c          675 g          689 t
ORIGIN      
        1 aattgattac cgatcgtcgc catgccgagg ttatgcgtca gttccacgag cgtatcgacg
       61 gcattgcgga tcatgcgctc tggcgcaccg gcattctgca cgcgctgggc gagcgctttc
      121 tcggcccggc gctccggcag gctcgcgcct tcgctctcgt tgatgttgtt cacatacaag
      181 cgatagcctt tgtcagtcgg aacccgcccg gcgctagtat gcggctgcgt gatgtagccg
      241 agccgctcca actcagccat ctccgcccga atggtggccg aactgacatg gaagagcttg
      301 gccagcagca tactgccgac aggactggcg acctcagcat attgctctac gatagcgttt
      361 aaaatcttct gttgacgctc tgtcatttag cgctattata tcgtttttag cactcaagag
      421 tcaagagtgc taagcggcgc tgtctctggc gaaaatacgt agatctcttg ctccttatga
      481 taattttact cattaaggag aatagtaagc atccgctcat acttcctgta aaatcacccg
      541 tgccagagtc atgccagagt cgtatgaaaa catatgtttt ggcatgactc tcacacgact
      601 ctgggatggg cctttttaga gactttttgc ctattacatt tttactcctc aacgacttct
      661 ggacgtagcg actctttggc attataatta gtttttcata tgcatcattc tctcaatgca
      721 aaccaaattt caaaaataaa gtcctggctc ggcaccagct cactcaatat ttttggcagg
      781 ccttttgccg gtaaagatac tcagggcaag atattgtccg acatgttcga cggacaattg
      841 ctcggtggcg gtgacatctt gcgcaatagc accatacctg aacgatcaca gcaagctttg
      901 aagcaaggat tgctcatacc aactgatgag tatttggaaa tagtgcttcc ctactttagt
      961 caggatgcgt tcaaagataa gccgttcatc ctcagctctg tcggtcgatg gcatggggaa
     1021 gagcccggag tacttgaggc gaccgagcgt tctggccatc ctataaaagt ggtgctttac
     1081 cttgatatac cagaagaagc tgcacgtgtg agacgacaca aaagccgtga ggaacgttca
     1141 cgtggtgatc gcggcagccg acatgatgat gctcctgaaa tctttgagac acgcttaaaa
     1201 gaatttcgtc agaaaacact acccgttatt gaatattatc aagcgactcc gctttttgct
     1261 cacatcgatg ccaatcgtcc aggcgttgaa gttacagccc agattctgga agagttatta
     1321 gctcgtgcgg aacataagaa gcccgaatag ttgactctag atatactccg aggcggaaaa
     1381 tacccgcggc cgccgcactg ccagccgtag aaaaatggcg tgagcatgca tacttaaagc
     1441 gatcatggcg aaggatagca gggcatacaa aaagctgtta aggctcagcg gactggaagc
     1501 actggcataa gcagcggtga tactgtaggg aacgattcgt ttgaaaaata tatcgataaa
     1561 aattaaccaa accacggctg caacgattct cagtaataag tactcaatgg tggttttcag
     1621 caacgcttca cgcttagcgt gtacgtaatg caactcgtcc tgtagctccg cgatgctact
     1681 gagcgccttc accacagttt cgatgacaaa atacacgacg aaacctatga cgccccagaa
     1741 ggcgagtaga ggccaggtat tttgcaacga attagttccg gcacttggag gcgcctcgcc
     1801 cggcaaggtt aaggcggact gctgagcggt actttgtagg ccgaccagct gttgttggac
     1861 ctggctgttg tgtagattga agaggacaat aaccccgact attacgacaa gccctagaga
     1921 caccgccagc aaaccactga gccatgacgg cgtaaagagc gctatgagca gtttttggtt
     1981 gagtcttttt tcggtttcgt ccataacttg gtagctgtac cttactatac ctaattacct
     2041 ggcattgaga cagtgctagt cgcgctttaa cagcacggtg aatgcttccg cggggatgtc
     2101 gaccttacca aagcgcttca agcgtttctt gcctttggcc tgcttagcta gcaccttttt
     2161 cttgcggctt acgtcaccgc cgtacaagcc agtcgtaaca tctttacgat aagcgctgag
     2221 gtcttcgcgg gcgataaact tgccgccaat cgctgcctgc aaagctacct gaaagttttg
     2281 ccgcggaata acatccttca atttctgaac gatgtcacga cctaacattt gcgcctcgct
     2341 gcggtgcacc ataaggctca gggcgctcac catttcaccg gccacgtaga aatccagtcg
     2401 caccagatct tcggctcggt aatcggccag ctcgtaattg aacgagccgt acccgctggt
     2461 cagacttttg agttgatcat aaaaatcggt tagcagatta gccagtggcg cttcaaaagt
     2521 gataagcgcc cgctggtcaa catacgagat gttattctgc cggccgcgct tcgcgctcac
     2581 caactgaatg actggtccga catactcctg cggcacgata atctcgccga cgatccaggg
     2641 ttcacggatt tcgttgattt tggctggatc gggcaagtcg gcggcgcttt tgatgtccag
     2701 ctcttcgccg ctcactagcg ttacctggta gtcagttgac gg
//
LOCUS       DATNGP010000083         7135 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_3097531, whole genome shotgun sequence.
ACCESSION   DATNGP010000083 DATNGP010000000
VERSION     DATNGP010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 7135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7135
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3097531"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..796
                     /locus_tag="VN778_02035"
     CDS             <1..796
                     /locus_tag="VN778_02035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49784.1"
                     /translation="PVFEDQLFVSLGLANLYGWIAYTIYDDFLDDEGQPELLSAANAA
                     LRYSLREFELALPRNQTFQALVQKTFDTMDSANAWEVRHCRFAVDDHILKLRKLPNYA
                     GLECLAERSLGHALTPLAILIALGEPLDSPAVLTCFQAMRHYLVARQLNDDLHDWEED
                     VARRQITYVVSEILRDLKPRIKQQDVRRLLGVMRQQFWHLSLPRLSQTITEHVEQARE
                     ILADHPLLKPSNALTDLLDIVAGINRETLATVAQAEEFLKSYRANK"
     gene            797..1840
                     /locus_tag="VN778_02040"
     CDS             797..1840
                     /locus_tag="VN778_02040"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HXR49785.1"
                     /translation="MENILSYQKTLAADLAQYFSDRADMKNTNPWHVDITAQLEQYLT
                     GGKLLRGSLVCFAHEAFGGELLQAARRTAMALELTHSALLIHDDVIDRDALRRGRAAM
                     HLRYQALATTADAAHFGESMAICVADAAIFLAFERLASVDIPAVPRQRLITFFTKALV
                     TVCAGQMLDIELGYMRGGVKKRDILMVMEQKTAAYSITLPLVAGAILADQPDTVIRAL
                     SDLGRLVGIIFQIRDDELGVFGDPEKTGKPAGSDIKEGKKTLLYYYLMQRCSGSEKRR
                     LLSIFGNQQVSSDNIDYVRQVMIRAQVPSTVNREVKALSRQAAKLIADLPLTPSGQAD
                     LLGFLSFCAKRDV"
     gene            complement(1837..2604)
                     /locus_tag="VN778_02045"
     CDS             complement(1837..2604)
                     /locus_tag="VN778_02045"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HXR49786.1"
                     /translation="MAIFAVLFEGGGELGHYQVVDCIYGGRPARVLYSGNRLAAQSGM
                     ARDGKSELLFDYNQRFLELIRGLIPKRLLLIGGGAFTLPKVLMDEFPRMSIDVVEPDA
                     KLPDIAQAYFDFKPNKRMHMYHDDGRNFLTNSAAAYDVIVIDAFENTEVPLSLQTAEA
                     VRSLRQHLRPGGVVAINVIAAYSGERSRVLRRVVAAFQDAFPEVRIFPASPSLSLWTP
                     QNFICVGYDGQLDLEPYVRYAAQDLPAVETDDLIRDA"
     gene            2705..3109
                     /locus_tag="VN778_02050"
     CDS             2705..3109
                     /locus_tag="VN778_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018770043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HXR49787.1"
                     /translation="MNVPRNWHRAHKESLSRGDRAADKLRNGMGSWTFVFAFVAFMGA
                     WAILNSIASLHHWDKYPFILLNLFLSMLAGLQGAILLIAAKRVDGISAAMAQHDYETN
                     VDAKKEIERLMAINEQQLKLIEQLVKDLEHSR"
     gene            complement(3333..4244)
                     /locus_tag="VN778_02055"
     CDS             complement(3333..4244)
                     /locus_tag="VN778_02055"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HXR49788.1"
                     /translation="MKSIDKIIQQADAWQRRHRFPAFAYAVVKKYGEDEAGYQAALLT
                     YYSFLSLFPLLLVLTTVTTMIAGSHSELQNTLVKSTTNYFPVLGNQLSSHIYTLHKNG
                     FALFIGVLFTLYGTRGIADAFRHGVQHIWGIPKAQWDGFPESTLKSLALVIIGGAGFL
                     LASIAAGLGGAFGHGLAFRLLALVINFLILFLLFSFLLNASLPRHVAIKEVRTGAVVA
                     ALGLVILQTLGGILISRELKRLDALYSYFAISLGLLFWIYLQAQVLFYAVEIAVVSSQ
                     KRWPRSLSGHNLTPTDKAAKAYLERQV"
     gene            4432..4563
                     /locus_tag="VN778_02060"
     CDS             4432..4563
                     /locus_tag="VN778_02060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49789.1"
                     /translation="MSNDEAEAIEAKFEKKWADNERHNFISRMERKLYRKERSGRTQ"
     gene            complement(4621..5475)
                     /locus_tag="VN778_02065"
     CDS             complement(4621..5475)
                     /locus_tag="VN778_02065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49790.1"
                     /translation="MAEEKKDDAGAQPQPGQVVTPGSSSSQPTPPLIQPVGLPRDESQ
                     RVVPSLTREALHPVAPDIPDEAPSPPEPDPPAPALLPPSAPAPPAPETTTPAHESPTP
                     APIPGPPPPSPEPPSDPEPAPEPPAGVAEDEGGISWVAAEFVAHEKSASWYMKLAAIA
                     IAIAAIIFLLTRDPISTAVAPVCALFLGILAARHPRELRYRMSDHGISVGHKHYGYED
                     FRSFSLTPEGALLGIALMPLKRFAPLLTIYCAPKDQDKIISVLSDRLPLEEHKPDAIE
                     QLMRRIHF"
     gene            5605..5694
                     /locus_tag="VN778_02070"
     tRNA            5605..5694
                     /locus_tag="VN778_02070"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5639..5641,aa:Ser,seq:tga)
     gene            5735..5827
                     /locus_tag="VN778_02075"
     tRNA            5735..5827
                     /locus_tag="VN778_02075"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5769..5771,aa:Ser,seq:gct)
     gene            5959..6393
                     /locus_tag="VN778_02080"
     CDS             5959..6393
                     /locus_tag="VN778_02080"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HXR49791.1"
                     /translation="MDDKLEQQIIINAPITTVWAVLTRPEHIIKWFSDRVDIDVRPGG
                     KGLLSWDTYGDAPLEVVRVDEPNLFSFAWVGADEETREADQQTLVEFRLTEVAGGTKL
                     LLTETIYGKLKLTEEKKNSLFEKHRSGWSVFADRIKQHAEAQ"
     gene            6406..6744
                     /locus_tag="VN778_02085"
     CDS             6406..6744
                     /locus_tag="VN778_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007916898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXR49792.1"
                     /translation="MVATTFNDKIWFAIAEPSRRNLIDILIFQGEASSSKLAKEVSFS
                     RQAVAKHITVLKKVGLIRERRAGKEIQFSIEPSGLTAAIQELSAAAELWDARLQKIKQ
                     IAEAIHQDNC"
BASE COUNT         1815 a         1758 c         1920 g         1642 t
ORIGIN      
        1 gccggttttt gaggaccagc tattcgtgag cctggggctt gctaatctgt acggctggat
       61 agcctacacg atttacgatg attttttaga tgacgaaggt cagcctgagc tactcagcgc
      121 cgccaatgcc gcgctgcgct acagcttgag agagtttgaa ctggccttgc cgcgcaacca
      181 gacctttcag gcgctcgtac aaaaaacctt tgataccatg gatagcgcca acgcctggga
      241 agttcgtcac tgccgcttcg cggtagatga tcacatccta aagcttcgca aattgccgaa
      301 ctacgccggc ctcgaatgcc tggcagaacg ctctctcggg cacgccctca cgcctctggc
      361 aatcctgatt gcgcttggtg agccactcga tagtccggcg gtactgacgt gctttcaggc
      421 tatgcgccac tatctcgttg cgcgtcagct caatgatgac ctccatgatt gggaagagga
      481 cgtcgccaga cgccagatca cctacgtggt cagcgaaata ttgcgcgacc tgaagcctcg
      541 gatcaagcaa caagatgtac ggcgtctgct gggagtcatg cggcaacaat tttggcatct
      601 gagtctgccg cgactcagcc aaactattac ggagcatgtc gagcaagcgc gtgaaatatt
      661 ggccgatcat cccctgctga agcctagcaa tgcattgaca gacttgctcg atatcgtggc
      721 cggcatcaat agggaaacgc tggctactgt cgcccaggct gaagaattct taaaaagtta
      781 tcgggcaaat aagtaaatgg aaaatatact cagctatcag aaaacactgg cggccgatct
      841 ggcacaatac tttagcgatc gagccgacat gaaaaacact aatccctggc acgtagacat
      901 tacggcgcag ctggaacagt atttgaccgg cggcaagtta ctgcgaggtt ctttggtttg
      961 ttttgcgcat gaggctttcg gcggcgaact cctgcaggcc gccaggcgca cggccatggc
     1021 tctggagcta actcattccg ccttgctcat ccacgacgat gtcattgatc gggatgccct
     1081 aagacgcggg cgggctgcta tgcaccttcg gtaccaagct ttggctacta cagccgatgc
     1141 cgcacatttc ggtgaaagca tggctatttg tgtggctgat gcggctattt tcctagcttt
     1201 tgagcggctg gcgagtgtcg acatccccgc ggttccaaga cagcggttga tcaccttttt
     1261 cactaaagcc ctcgtcaccg tgtgtgccgg ccaaatgctc gatatcgagc tgggatacat
     1321 gcgcggcggg gtaaaaaaac gcgacatact gatggtcatg gaacaaaaaa ccgctgccta
     1381 ttcgataacg ctgccgctgg tagccggggc aatcttggcc gatcaaccgg acacggttat
     1441 ccgcgcattg tcagacttag gcaggctggt cggcatcatt ttccaaatcc gcgacgacga
     1501 attgggcgtt tttggtgatc cggagaaaac cggcaagccg gccggctcag acataaaaga
     1561 aggcaagaaa acgctgctgt attactatct gatgcagcgt tgctccggta gcgagaagcg
     1621 gcggctactg agcatcttcg gtaatcaaca ggtgagttcg gataacatag actatgtgcg
     1681 ccaagtcatg attcgtgcac aagtgccgag caccgttaat cgtgaagtta aagccctgag
     1741 tcgccaagcc gccaagctca ttgccgacct gccattgacc cctagcggcc aagcggactt
     1801 gcttggcttc ctaagcttct gcgccaaacg cgatgtctaa gcatcacgta taagatcgtc
     1861 ggtttcaact gccggcagat cttgagcggc atagcgcacg tatggttcta gatctagctg
     1921 gccgtcatag ccgacgcaga tgaagttttg aggcgtccag agcgagagtg aagggctcgc
     1981 cggaaaaatg cgcacttcgg ggaacgcatc ctgaaatgct gctaccacgc gtcggagtac
     2041 ccgggagcgc tctccgctgt aggctgcaat gacattgatg gccacgacac cgcccggtcg
     2101 caagtgctgc cgcaggctac gaactgcctc ggctgtttgt aaagataggg ggacctcggt
     2161 attttcgaac gcatcgataa caattacgtc atacgcagcc gctgagtttg tgagaaaatt
     2221 acggccatca tcgtgataca tgtgcatgcg cttattaggc ttgaaatcga agtaggcctg
     2281 cgcgatatcg ggtaacttag cgtccggttc caccacatca atgctcatcc gcgggaattc
     2341 gtccatcaag actttcggca gcgtaaacgc accgccgcca atcaacagca agcgctttgg
     2401 gattaggccg cgtatcagct cgagaaagcg ctgattataa tcgaacagca gctctgattt
     2461 gccgtcgcgg gccatgccgg actgagctgc tagccggttg ccgctataga ggacgcgcgc
     2521 tggccgcccg ccgtagatac agtccaccac ttggtagtgc cccagctcgc ccccaccttc
     2581 aaaaagcact gcaaaaatcg ccatgctttt agtatagaat ctcttgctca taaagctgcc
     2641 atgcaagctg tatcatcaat tctttaggac tcgggattga tcttggttat gctatacttc
     2701 gcgtatgaat gtgccacgca actggcatcg agcgcacaaa gagagcctgt cccggggtga
     2761 ccgggcggcc gataagctac gtaacggcat gggtagttgg acatttgtct tcgctttcgt
     2821 ggccttcatg ggggcctggg ctatcttgaa cagcatagcc agcctgcacc actgggataa
     2881 gtaccccttt atcctgctca acctgttttt gtcgatgtta gctggcctac agggggcgat
     2941 tttattgatt gcggccaaac gagtcgatgg catcagtgcc gctatggctc aacacgatta
     3001 tgaaaccaat gttgatgcca agaaagagat cgaacggctg atggctatca acgagcagca
     3061 gctgaaatta atcgaacaac tcgtgaaaga tcttgagcac tccaggtaag cttgtgcgcc
     3121 tgcttaaaat cacttcccgt atgtaaaaaa actaacagta gcatctcagc taaacaacct
     3181 atgctacagg tgtaagcagc ggtcgagcaa ttaggccaag gggcttacgg ggagttttac
     3241 aaacgggagg aaccatgcca gttcagcaaa ctacacgatc cagagcgacc gtacggcagc
     3301 gtgcttggtg gcgacagtac ctgatgtact agttagactt ggcgttctaa ataagccttg
     3361 gctgctttat cggtgggcgt taaattatgg ccgcttaggc tacgtggcca tcgcttttga
     3421 ctgctcacga ccgctatttc cacggcataa aagagcactt gagcctgcaa atatatccag
     3481 aaaagcaagc ctagggaaat ggcaaaataa ctatacaagg cgtcgagtcg tttcagctca
     3541 cgcgagatca agataccgcc gagtgtttgc aagattacca gtcctagggc ggcaacgaca
     3601 gcaccggtgc gcacttcttt aattgccacg tggcgcggca agctggcgtt tagtaaaaaa
     3661 ctaaagagca ggaaaagaat caagaagttt atgactagtg ccagtaagcg gaaggccagt
     3721 ccatgaccaa aagctccccc caagccggca gcgatcgaag ccaggagaaa accagcaccg
     3781 cctatgataa ccagagccag gctcttgagg gtggactccg gaaagccgtc ccactgcgct
     3841 ttcggaatgc cccaaatgtg ctgcacaccg tgtcggaaag cgtcggcgat tccccgggtg
     3901 ccatagagag taaaaagtac gccgataaat agcgcgaaac cgtttttatg caaagtataa
     3961 atgtgagagg atagttggtt gccaagcacg ggaaagtaat tggtcgtgct ttttactaga
     4021 gtgttttgta gttctgaatg actgccggca atcatcgtgg tcacagttgt caacaccagg
     4081 agcaggggaa ataaggacaa aaaggagtag tacgtcagta gggctgcctg atagccggct
     4141 tcgtcctcgc catacttttt gacaactgcg taagcaaagg cggggaagcg atgccttcgt
     4201 tgccaggcat cggcctgctg gatgatttta tcaatgcttt tcatcttgga cgattaatct
     4261 actcactaca gcataacagg aaatcggctc ctatccattt gttagaaaac ttacagctgt
     4321 tctaaagcca taagcctaat aataccctca gaacttagcg ctctggtacg gccgcacatg
     4381 tactaagcaa gcaaggttct aaagtacaaa ttaaaaggag ggctaaagca aatgtctaac
     4441 gacgaagccg aagcaataga agcaaaattt gaaaaaaaat gggccgataa tgaaagacac
     4501 aatttcatta gccgtatgga gcgcaaactc tatcgcaaag aacgttctgg ccgtacccag
     4561 taatctgttg aacttgttga ttcgtggaac cgtctctaaa ggggcggttt ctttttataa
     4621 ttagaaatgg atgcggcgca tcagttgttc gatagcgtcc ggcttatgtt cttcgagcgg
     4681 taggcgatcg ctcaatacgc tgattatttt gtcttgatcc ttcggtgcgc agtaaatggt
     4741 cagcaaagga gcaaaacgtt ttaagggcat aagggcaatc cccagaagtg cgccctccgg
     4801 cgtgagcgag aaggagcgaa agtcttcgta gccgtagtgc ttgtgcccca cactaatacc
     4861 atgatcgctc atccgatacc gtagctctct aggatgcctg gcagccaaaa tgcccaagaa
     4921 caaagcacag actggcgcca cggcagtgga gataggatct cgagtcagta agaaaataat
     4981 cgccgctatg gctatggcta tggccgccag cttcatgtac caactggcag acttttcatg
     5041 ggctacaaat tctgcagcta cccagcttat accgccttcg tcctctgcaa cgccagcggg
     5101 aggttccggg gccggctcgg gatctgatgg gggttctgga gatggcggcg gagggcccgg
     5161 gattggcgct ggggttgggg attcgtgggc aggagtagtg gtttcgggcg cgggtggcgc
     5221 tggagctgaa ggtggtagca atgcgggagc aggtggatct ggttcggggg gagatggcgc
     5281 ttcatcgggt atatcaggcg ctactgggtg cagggcttca cgcgtcaaag agggcactac
     5341 tcgctgagat tcatctcttg gtaagccgac gggctgaata agaggtgggg taggctgcga
     5401 tgaggagctg ccgggcgtga caacctggcc cggctgtggc tgggcgccag catcatcttt
     5461 tttctcttct gccataagcg ttattgtacc gcagtcaccc ttgtttttcc atcttggccc
     5521 gtccgggatt tctaagggct tattgagaat ttggataacc tgcaggcggc ttaacacctc
     5581 tcgccatggt ataattttgc ggttggaagg gtgtccgagc ggtttaagga gcctgtcttg
     5641 aaaacaggtg tgccggaaac ggtaccgtgg gttcgaatcc caccccttcc gccagtgctc
     5701 gttccacgtt acaatagcag aacgtgtcag atacggagag gtacccaagt ggctgaaggg
     5761 gtcggtttgc taaatcgata ggggggagca atcccccgcg agggttcgaa tccctctctc
     5821 tccgccaaga aagttatgca tttttttgaa acttactcct tatagggggc cgttataggt
     5881 gatactgcaa ctaaaggttg catttcaaaa tgagatgtgc aacaataggt tgcacattat
     5941 actataagga ggttcgtagt ggatgacaaa ttggaacaac aaatcatcat taatgctccc
     6001 attacgacgg tatgggcggt attaactagg ccagagcata tcattaaatg gtttagcgat
     6061 cgagtagata ttgatgtacg acctggcggc aagggcctgc taagctggga tacatacggc
     6121 gatgcccccc ttgaagtagt ccgcgtcgac gagccaaacc ttttctcatt tgcatgggtg
     6181 ggagcagacg aggaaactcg agaggccgat caacaaacgc tcgtagagtt taggctaact
     6241 gaagttgcag gcggcacaaa gctcttactg acagagacga tctacggcaa actgaaactt
     6301 accgaggaga aaaagaatag cttattcgag aagcatcgtt ctggctggag tgtctttgca
     6361 gatagaataa agcagcacgc tgaagcgcaa taggaaagtt tctttatggt agcaacaaca
     6421 tttaacgaca agatttggtt cgccattgcc gagccaagtc gacgcaatct gatcgatatc
     6481 ttgatctttc aaggcgaagc ttcatcaagc aagttagcca aggaggtgtc cttcagccga
     6541 caagccgttg ccaaacatat aaccgttttg aaaaaggttg ggttaattcg ggaacggcgt
     6601 gccggcaaag aaattcagtt ttcgatcgag ccaagtgggc ttactgcagc tatccaggag
     6661 ttatctgcag cagcagagct gtgggacgca agattgcaaa aaattaaaca gatcgccgaa
     6721 gccatacatc aagacaactg ttagcacaat gtccaaaccc ctatcagcca gatacttttc
     6781 ttatactgca ggagccgttg atacctctgc tcaatttccc cgcatgagag tcatgtcaaa
     6841 gtcgtatgca aacatatgtt ttgatatgac tcttgtttga ctctggcata gctggttttc
     6901 cggcaccctc aaagaagggg taacgagctt aaagtgaaga cctgaccgat cgacgggcag
     6961 tacgatcttg cgcttcactt agtgacgaaa ggcgagccac agccacaacg cgacaaaaaa
     7021 gagcacaaca ataacactga ctatctgctg ccagcgaggc tcggggatat gagtgcggaa
     7081 gttatcttgc tgctttttca tgctcgtttt ggttgaatac gaacggccgt gccgt
//
LOCUS       DATNGP010000084         3714 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_86804, whole genome shotgun sequence.
ACCESSION   DATNGP010000084 DATNGP010000000
VERSION     DATNGP010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3714
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86804"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            221..439
                     /locus_tag="VN778_02090"
     CDS             221..439
                     /locus_tag="VN778_02090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49793.1"
                     /translation="MVRYHENQPPTTQAPDQTIPPAPVPEQEYEVTSALNPNKPSHLR
                     LVALIMGGDKQRLGGHILTADKLRSRSV"
     gene            471..1493
                     /locus_tag="VN778_02095"
     CDS             471..1493
                     /locus_tag="VN778_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010026442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HXR49794.1"
                     /translation="MNIYEALPRPFFVLAPMDDVTDTVFRRVIGKCAAPDLYFTEFVN
                     VDGLQSPGRQKLLKKLRFTSSEQPLIAQIWGKEPDNFYKTARELADGTFARELGLPEG
                     VNFAGVDLNMGCPEKTAVKNGTCSALINDRPKAEVVINATREGLASRLPLSVKTRIGF
                     SQIDMSWIEFLLGQKLTALTVHGRTRKEMSKVPAHWGQIGEARLLRDQIAPETLIIGN
                     GDVRDRAHGLELIAKYQIDGIMIGRGVFHDPFAFAETSPWPGYTAEQRLDLYKQHVEL
                     FAETWQHNEHPMIVLNKFCKIYVSGFDGAKELREQLMAAGSTDELLSILRVRLRARAL
                     EMQSSN"
     gene            complement(1450..2163)
                     /locus_tag="VN778_02100"
     CDS             complement(1450..2163)
                     /locus_tag="VN778_02100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49795.1"
                     /translation="MSPERITTTFEAMNTPPQVPYKDYLSDATQALEAAISTEKKDGL
                     DVAVDQYRVAADKFNEAIMKQPVSSERELKKHRDDPFLWLQLGRCLAKEIESGDVQPY
                     RNPYEPVLDESGEILEMRLHDRRKELSENVSKIFTEAWDGVMHAPVADEIKINSGIVA
                     ADGLRIVHDISQAETVVEEVDQLLEPLEDTSSKVVELFKPSESFKQSTDHRLYMAREL
                     ANQAVLNLTTASQELLLAA"
     gene            complement(2352..2427)
                     /locus_tag="VN778_02105"
     tRNA            complement(2352..2427)
                     /locus_tag="VN778_02105"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2392..2394),aa:Ile,seq:cat)
     gene            2509..2958
                     /locus_tag="VN778_02110"
     CDS             2509..2958
                     /locus_tag="VN778_02110"
                     /inference="COORDINATES: protein motif:HMM:NF016839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HXR49796.1"
                     /translation="MQTKIEPYQQKAVDFLRRLSDVRFAGQVLFVVIVLLISWSGVKT
                     IQTNYGLQKQISALRQQNDVQHLQNNNLALQNEYLNSNQYLELSARQNFGLAQPGEKE
                     IIVPQNVALAYTVNVPDNTSDKTTAVQPAYQRNLQSWVNFFFHRQSS"
     gene            3013..3087
                     /locus_tag="VN778_02115"
     tRNA            3013..3087
                     /locus_tag="VN778_02115"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3045..3047,aa:Met,seq:cat)
     gene            complement(3191..>3714)
                     /locus_tag="VN778_02120"
     CDS             complement(3191..>3714)
                     /locus_tag="VN778_02120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49797.1"
                     /translation="SSGNFQVFLQSTGSIDQYIKYTSGTWGSGAPATMSGIISQPWAF
                     QDATGNINFFFNTSGGSDHYEYSGGSWGSEVALSNSSLIYWPFAYHITISGTDYYVVI
                     VETASGITDWWRPTSGGTWSHETTATVTPTSGMVGYVDGSNNQNMFVNTLTTLNHYEN
                     SGAGWGGEQVYHD"
BASE COUNT          937 a          971 c          908 g          898 t
ORIGIN      
        1 acacacccgt ctcacgaggt cagcagcgaa tctgccgagt ctttctttga ggcattgcac
       61 ggccattctc ctcatcagtc ccagagttaa taatttttta atttcctgag tgcataatct
      121 tacagaaaga aaccagccaa aggcgttact ctagtatggt tgtgccaaat tatgcgtacg
      181 agcctggagt tacaaaacta ataagcaagg agtaacaaat atggtacgtt accatgaaaa
      241 tcaaccccca accacccaag cccctgatca aactattcca cctgctcccg tccccgagca
      301 ggagtacgaa gtcacttcgg ccttgaatcc gaataagcct tcccacttac gcctggtcgc
      361 cctaatcatg ggtggagaca agcaacggct tggtggccat attctaacgg cggataagct
      421 gaggagccgg tctgtttaaa ctcccctaat aagcctataa ttagagagtg atgaacatat
      481 atgaggcgct gccgaggcct ttctttgtct tggcgccgat ggatgatgtg acggatacgg
      541 tgtttcgccg ggttattgga aaatgcgcgg cgccagatct gtatttcacg gagttcgtga
      601 atgtcgacgg gctgcaaagc ccaggccggc agaagctact caaaaagctg cggtttacgt
      661 ccagcgagca gccgcttatc gcccagatct ggggcaagga gccggataac ttttacaaga
      721 cggcgcggga gctggcggac ggtacttttg cgcgagagct tggcctgcca gagggcgtca
      781 acttcgccgg cgtcgacctg aatatgggct gccccgagaa aaccgccgtc aaaaacggca
      841 cttgctccgc gcttatcaat gaccgcccga aggccgaagt ggttattaac gccacgagag
      901 agggcttggc tagccgcttg ccgctgagtg tcaaaacccg tatcggcttc agtcagatcg
      961 atatgagctg gattgaattc ctgctgggcc aaaagctgac agccttgact gttcacggcc
     1021 gcactcgcaa agaaatgagc aaagtaccgg cgcactgggg ccagataggc gaagcccgac
     1081 tgcttcggga ccagatagct cccgagacgc tgatcatcgg caacggcgac gtccgagatc
     1141 gagcccacgg gctggagctg attgcaaaat accagattga cggcatcatg atcggccgtg
     1201 gcgtctttca cgatcccttc gcatttgccg aaacaagtcc ctggccagga tacaccgccg
     1261 agcagcgtct cgatctctac aagcagcatg tcgaactctt cgccgaaacc tggcaacaca
     1321 atgaacaccc catgatcgtt ctcaacaaat tctgcaaaat ctacgtcagc ggcttcgacg
     1381 gcgccaaaga actccgcgaa cagctcatgg ccgccgggag cactgacgag ttactgtcta
     1441 tcctgcgagt taggctgcga gcaagagctc ttgagatgca gtcgtcaaat tgagcactgc
     1501 ttggttagcg agttcgcgcg ccatatacaa tctatggtcg gtcgattgct taaaggattc
     1561 agatggctta aagagctcaa ctaccttgga acttgtgtcc tcaagtggct cgagcagctg
     1621 atctacttct tcaacgacgg tttcggcttg agaaatatca tgaacaatgc gcaacccgtc
     1681 agcagctacg atgccactat tgattttaat ctcatctgca accggtgcat gcataacgcc
     1741 gtcccatgct tccgtaaaga ttttgctaac attttcagaa agctctttcc ggcggtcatg
     1801 caatcgcatc tccaggattt ctccgctttc gtcgagcacc ggctcatatg ggttgcggta
     1861 tggctgaacg tcaccactct cgatttcttt agcgaggcag cggcctagct gcagccacaa
     1921 gaacggatcg tctcggtgtt tttttagttc acgctcgctg ctcacgggct gcttcataat
     1981 cgcctcgttg aacttgtcgg cagcgacacg gtactggtcg acagctacat ctaaaccatc
     2041 ttttttctct gttgaaatgg ctgcttctaa tgcttgagta gcatccgata ggtaatcttt
     2101 ataaggtacc tgcgggggag tattcatggc ttcaaaagta gttgtgattc gttcaggaga
     2161 catttttatt gattactcat cttgaattat aagcctgcat ccattataac gccggaagtc
     2221 aaccatacgt aagcactttt gaatgtaaaa aatctcacaa agttgcccat ttcgtgaaaa
     2281 ttaaaaatag cgcccctcgt cggccagcgc ttaatcacat catatcataa gtattatggt
     2341 atgtcaactt ctggtggggg cggctggatt tgaaccagcg accaatcggt tatgagccga
     2401 ctgctctaac ccctgagcta cgcccccgtg gaaatggcag tcagtggtag ataacagatg
     2461 tgagtatacc agatttgggc aaaagttata taatggtaac gcgagtttat gcaaacaaaa
     2521 atcgaaccct atcagcaaaa agcggtagat ttcctgcggc gcttgagcga tgtacgcttt
     2581 gccgggcagg tacttttcgt ggtgatcgta cttctgatca gttggagcgg cgtcaaaact
     2641 atccaaacga attatgggct gcagaagcag atctcggcgc tccgccagca aaatgatgtg
     2701 cagcacttac aaaacaacaa tttggcgctg cagaacgaat acctcaatag caatcaatac
     2761 ctcgagctat ccgctcgaca gaacttcggc ctggctcagc cgggggagaa agaaatcata
     2821 gttccgcaaa atgtagcgct cgcttacacg gtgaatgtac ccgataacac ctccgataag
     2881 actacggccg tccaaccggc ttaccagcga aacctccagt cctgggtgaa ctttttcttc
     2941 caccgtcaaa gctcctgaat tgacataatc acctctgttt gttataattt cctgagctaa
     3001 aacgcccgat gacgcggggt ggagcagcgg cagctcgcgt ggctcataac cacgaggtcg
     3061 caggttcgag tcctgccccc gcaaccagct tgagataaca gatagaggct tgctaatttg
     3121 caaggcctct ttttatttac tacctctgtt attttggttc gagtcctgct tggttaaaat
     3181 tcagtcgcca tcaatcgtga taaacttgct cgccacccca gcccgcacca ctgttctcat
     3241 aatggttgag ggtagtaagt gtattcacga acatattctg gttgtttgag ccatcaacat
     3301 atcccaccat acccgacgtg ggggtcacag tcgctgtggt ttcatggctc cacgttccac
     3361 ccgacgtagg ccgccaccaa tcagtaatgc cgcttgctgt ctcaacaatc actacgtaat
     3421 agtcagtgcc gctaattgtt atgtggtatg caaatggcca gtagatgaga gaggagttag
     3481 acagtgccac ttcacttccc cacgatccgc ccgaatactc atagtgatcc gagccaccgg
     3541 aggtattgaa gaagaagtta atgttaccgg ttgcgtcctg aaacgcccag ggttgagaaa
     3601 taatcccact catggtcgct ggtgcgccac taccccaagt gccacttgtg tatttgatgt
     3661 attggtcgat actgcccgtg gactgtagaa atacctggaa gttgccagag gaat
//
LOCUS       DATNGP010000085         3015 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_1645049, whole genome shotgun sequence.
ACCESSION   DATNGP010000085 DATNGP010000000
VERSION     DATNGP010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3015
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1645049"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1293
                     /locus_tag="VN778_02125"
     CDS             <1..1293
                     /locus_tag="VN778_02125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49798.1"
                     /translation="NFLAVAGLVLSVAALSFSGTVPVAANGSGSFYLSPASQSVASGS
                     TLSLTLSEDSGSTSVNALQANLTYSSNLQFVSADYGSSAFSVQAASSGKNGSLTIARG
                     ATASVSGAHTVGVVTFKAVASGTATVKFGCINGGSCIDGDALVASANSSNVLTTQTNG
                     SYTITSSAANTSSSSGLPQISGVVVTDVTDSSAIVSWQTDQPATSVVNYGTTTKLGLS
                     AQAAGLTTSHRVVLSNSIVKGATYYFAVSSALSTGASSTSSVQHFTTTGFAVTIIVNN
                     KQGKPVAHATVTIGNQSATTNSNGQVVFQNVPANQQQVVIKVAGKETKRSITVGKVNP
                     ATKGYENQLFSLVAAQGNNTWLYGVLGAVVVLAGIVLFVVRPFSSGRGTASQPVSGVP
                     VIPVSSSSTDDTPPTLPDQLSAQEPGEVVTPQSNKPKS"
     gene            1314..2864
                     /locus_tag="VN778_02130"
     CDS             1314..2864
                     /locus_tag="VN778_02130"
                     /inference="COORDINATES: protein motif:HMM:NF012271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HXR49799.1"
                     /translation="MSAAKSSSNKYKPQLPKWLSDSTRRPVAAVAAAVVAILGAYFVV
                     TSLAATGTGTLTLSPSSSTVALGSTFTVTVSENSGTTATKTVQANMTYSSNLQFVSVD
                     YSTSAFGTQAQSTTGNGVVQIARASTTDLTGSQILGVITFKAISAGTGTVNFDPIAVG
                     TGSGNAIVSSTDNTNILGTQVNASYSITDQTAPAIPGGLTVASHAVTSIGLSWTASTD
                     NVGVTGYKIYRNGTQIATTTGTTYTNTGLTPNTTYTYTVAAYDAAGNVSAQSPGVTGT
                     TDPDTTAPTVPGTPTQSSRTMTTMAISWTASTDNVGVTGYKVYRNGTQVATTTTTNYS
                     DTGLVPGTTYTYKVAAYDAAGNTSAQSTGASLATAPDTQAPTVPGSLTVTSTTSTSVS
                     LSWTASTDNVGVTGYKIYRNSSTTALATVTTTSYTNSGLTGSTAYTYQVSAIDAAGNE
                     SAKSSVISTTTPASGDLNGDGHINIFDLSLFLNHWQETGTGLPEDFNGDNLVNIFDLS
                     ILLNNYGT"
BASE COUNT          720 a          926 c          741 g          628 t
ORIGIN      
        1 aatttcttgg cggtagctgg tctagtgtta tccgttgcgg ccctgagttt tagcggcacg
       61 gtacccgtgg cggcaaatgg cagcggatcc ttttacttgt cgccggccag tcagagtgtg
      121 gctagcggct cgacattgtc gcttacgctg tccgaagata gcggcagcac gtcggtgaac
      181 gccctccagg caaacctgac ctattcatcg aatttgcagt ttgtcagtgc tgattatggc
      241 agtagcgcct tcagcgtgca ggccgcctct agtggcaaga acggctcgct gaccattgcc
      301 cgcggtgcta ccgcctcggt cagcggcgcc cacactgttg gtgtcgttac tttcaaagcg
      361 gtggccagcg gcacggccac ggtgaaattt ggctgtataa atggcggctc ctgcattgat
      421 ggtgatgctc tagtagcttc cgcaaacagc tccaatgttc tcactacgca gaccaacggc
      481 agctacacga taacgagttc agcggccaac acgagcagct cgagcgggct gccgcaaatt
      541 tcaggcgtgg tcgtcaccga tgtgactgat agcagtgcca tcgttagctg gcaaaccgac
      601 cagccggcca cctcggtggt gaattacggc acgactacca agctcggctt atcggctcag
      661 gctgccggac taaccaccag ccaccgcgtg gtgcttagta attctattgt taagggcgca
      721 acatactatt ttgctgtcag cagtgccttg agcaccggag ccagttcgac cagtagtgtt
      781 caacacttca cgaccacggg ctttgcagtt acaatcatcg tgaacaacaa gcagggtaag
      841 cccgttgctc atgccacggt aactatcggc aatcaaagcg ccaccaccaa cagcaatggc
      901 caagtcgtct tccaaaacgt cccagccaac cagcagcaag tcgtgatcaa ggttgccggc
      961 aaggaaacta agcggtcaat caccgtgggt aaggttaacc cagccaccaa aggctatgaa
     1021 aaccagcttt tctctctcgt ggctgctcaa ggcaacaata cttggttgta cggcgtgctc
     1081 ggcgcggtag tggtgctggc cggcatcgta ttgtttgtag tcaggccgtt ttcatctggc
     1141 cgcggcactg ccagccagcc agtctcaggc gtcccagtga tcccagtatc gtcgtcatca
     1201 acagacgata cgcctcccac gctacccgat cagctgtcag ctcaggagcc cggggaagtg
     1261 gttactccgc aaagtaataa acctaagagt taaactaaaa ataaggacat acgatgagtg
     1321 ccgcaaaatc ttcgtccaac aaatataaac ctcaattacc caagtggctg agcgatagta
     1381 ctcgaaggcc agtggcagca gtcgctgcgg ccgtggtggc tattctgggc gcctatttcg
     1441 tggtcaccag tctggcggct accggcacag gtacgctcac cctgtctccg tcgagctcga
     1501 cggttgctct cggttcgaca tttacagtaa ccgttagtga aaatagcggc accacagcaa
     1561 caaaaaccgt ccaagccaat atgacgtact cctcaaacct gcaatttgtt agcgtcgatt
     1621 acagcactag tgctttcggt acgcaagccc aatccacaac tggcaacggg gtagtacaaa
     1681 tagcccgcgc ttctactacc gatctgaccg ggagtcagat tctcggcgtt attaccttca
     1741 aggctatcag cgccggcacc gggacggtta attttgaccc gattgcggta ggaaccggca
     1801 gcggtaatgc tattgtaagc tccacagata acactaatat tttgggtacg caagttaacg
     1861 cgtcatattc aattaccgac caaactgctc ccgctatccc cggcggccta acagtggctt
     1921 cccacgccgt cacctcgatt ggcttgagct ggaccgccag caccgataac gtcggcgtca
     1981 ccggctacaa gatttaccga aacggtaccc agattgctac gacaacgggt actacgtaca
     2041 cgaacacggg cctgacgccg aacacaacct acacctacac cgtggctgct tacgatgcag
     2101 cagggaatgt ctccgcccaa tccccgggtg tcaccggcac aaccgaccct gataccacgg
     2161 ctcccacagt ccctggcacg cccacccaat ccagccgcac gatgaccacc atggctatca
     2221 gctggaccgc cagcactgat aacgttggcg tcaccggcta caaagtctat cgaaacggta
     2281 cccaggttgc taccaccacc actaccaact acagcgacac cggcctcgtt ccaggcacta
     2341 cttacaccta taaagtagcc gcctacgacg ctgcaggcaa tacctcggcg cagtccaccg
     2401 gggcctcatt agccactgca ccggataccc aggcaccaac ggtgcccggc agcttaacgg
     2461 tgactagcac cacctcgacc agtgtcagcc tcagctggac cgccagcacc gataacgtcg
     2521 gtgtcaccgg ctacaagatt tatcgcaaca gcagcacgac cgccttggcg accgtgacca
     2581 ccacatcgta cacgaatagc gggttgaccg gttctacagc ctacacctac caagtcagcg
     2641 ctatcgatgc agccggcaac gaaagcgcca aaagctctgt catcagtact accacccccg
     2701 ccagcggcga tctcaacggg gacgggcata ttaatatctt cgacctcagc ctcttcttaa
     2761 accactggca ggaaaccggg accggtttgc cggaagactt caatggtgac aatctcgtca
     2821 acatcttcga cctcagcata ctgctcaaca actacggtac gtagacccca ggtgatagtt
     2881 ggtgatgact tttacgtcta aatcatatcc gaaacactca ccaagcaaga cgttttcccg
     2941 gctgatggcc gcccttgtaa tcatcgtctt tactactctc ggttcccatt ttctctttac
     3001 tagccgagcg gctac
//
LOCUS       DATNGP010000086         3431 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_3887414, whole genome shotgun sequence.
ACCESSION   DATNGP010000086 DATNGP010000000
VERSION     DATNGP010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3431
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3887414"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..635)
                     /locus_tag="VN778_02135"
     CDS             complement(<1..635)
                     /locus_tag="VN778_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HXR49800.1"
                     /translation="MSAQNQPRIEVLLDEVVKRKASDLHLQVGLPPMIRVDGSLIPVN
                     GADPLDEEAIEVLIFAILDEDQKQILLKDKEFDFSFAYGDLGRFRVNAFHERGNMAAA
                     LRLIPNEILTIEQLGLPDIVNKFSDYPRGLVLVTGPTGSGKSTTLAAMIHKINNERAS
                     HIITIEDPIEYTHRSQKSVIAQREVHYDTYSFSAALRSALREDPDVVLIGE"
     gene            complement(670..2466)
                     /locus_tag="VN778_02140"
     CDS             complement(670..2466)
                     /locus_tag="VN778_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HXR49801.1"
                     /translation="MSVLSASTEQQVTDTLVKSEMLSEDKLAAAKAAAGKVGQPLLGY
                     LVKNGLITDEQLTKANATVTKMPYVNLLSAKVDPKVLQLLPKEIAERYMAVPLGEMQH
                     RLVVAMLDADNVQAVDFLSNRIGRPLKVYVASEAGIRQVLKQYSARLDTQMGAAFGAG
                     LTPTEAKETAKEPGKKAKKIENLVEDSPISKALSAIMEFAARNRASDIHIEPLEKELK
                     IRCRIDGVLREVTRLPKSTEPALISRIKILSNLKIDEHRIPQDGNFTILVDGKGIDLR
                     IAISPVVWGEQVVIRLLDKTGTSFKLEDMGYTGRALRAIRKGIKRPNGMILTSGPTGS
                     GKSTSLYALIQEIKSEQINIVTLEDPVEYKMDGINQIQVNPDVGLTFASGLRSILRQD
                     PNVVMVGEIRDKETAQLAVQAALTGHLVFSTLHTNSAAGILPRLLDMGIEPFLIASTV
                     NTVIGQRLVRRIADNVKETYQSTEAETAAIQATLGNLLPKTEADLAAASEDLGYKSLP
                     LAAQTAYTLSKGTDTPDTPGGYKGRMGLYEVFEVSEAIQAMIMKRATSAEIKKVAIAE
                     GMIDMRQDGYFKALDGRTTLNEVNRVAAEDTA"
     gene            complement(2479..2733)
                     /locus_tag="VN778_02145"
     CDS             complement(2479..2733)
                     /locus_tag="VN778_02145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49802.1"
                     /translation="MKPKDVALIGVIVFISAIVSWFASSAIFVKPKDRQQQVEVVQQI
                     TTDFPQPDTHYFNNSAFDPTKLITIGKNNNTSPFNGTPSQ"
     gene            complement(2730..>3431)
                     /locus_tag="VN778_02150"
     CDS             complement(2730..>3431)
                     /locus_tag="VN778_02150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49803.1"
                     /translation="QKQHRGSVMADREILTKRVAISKANAQIVGVVGVASFVTVFCLV
                     AAHAIWSQIGYQSRVTSAKEKAHQQLVKNISAYDNLTTSYKAFVGTPNNVIGGLSAGS
                     GDNDGDNAKIVLDALPPSYDFPALTTSIEKILTDRSLKITNITGTDDQLNQQKNTSSS
                     SPQAVNMPFSFTVSNASYSSVGQLITALEHSIRPIQIDTIELSGGANDMTLTVTAHTY
                     YQPGKSVNITKQVVK"
BASE COUNT          714 a          900 c          908 g          909 t
ORIGIN      
        1 atctcaccga tcagcacgac gtccgggtct tcacggaggg ctgagcggag cgccgcactg
       61 aaggagtagg tgtcgtagtg cacttcgcgc tgggcgatga ctgacttctg cgagcggtgc
      121 gtgtactcga tcggatcttc gatggtgatg atgtgcgagg ctcgctcatt gttgatcttg
      181 tgaatcatgg cggccagggt cgtggattta cctgagccgg tcggcccggt taccaggacg
      241 aggcctctcg ggtaatcgga aaacttgtta acgatgtcgg gcaagcctaa ttgctcgatc
      301 gtcagtattt cattggggat caggcgcagg gctgcagcca tattgccgcg ctcgtggaag
      361 gcgttgacgc ggaagcggcc cagatcgccg taggcgaagc tgaagtcgaa ttctttgtcc
      421 ttaagcagta tctgcttttg atcctcgtct aggatcgcga agattaagac ctcgatagcc
      481 tcttcgtcca gcggatcggc gccattgacc ggtatgagcg aaccgtcgac tctgatcatt
      541 ggtggcagcc ccacctgcaa gtgcaagtcg gatgccttcc gtttgacgac ttcatcgagc
      601 aaaacttcta ttcttggctg gttctgcgcg ctcatgattt tcccacctct cttgattgct
      661 tatgccgcat catgcagtat cctcggcagc gacccggtta acttcgttca gtgtcgtccg
      721 tccgtcgagc gctttgaaat atccatcttg acgcatgtca atcatacctt ctgcgatagc
      781 cacttttttg atttcggcgc tggtagctcg tttcataatc atcgcttgga tagcttctga
      841 cacctcaaag acttcataca gtcccatacg gcccttataa ccgcctggtg tgtccggggt
      901 atctgttccc ttgcttaaag tataagcggt ttgggcggct aagggcaagc ttttgtagcc
      961 taaatcctcg ctggcagccg ctagatcagc ctcggttttg ggtaaaaggt tgccgagcgt
     1021 cgcctgaata gcagccgtct ctgcttccgt tgattggtag gtttctttca cgttgtcagc
     1081 gatgcgccgc accaggcgct gaccaatcac ggtgttgacg gtgctggcaa tcaaaaacgg
     1141 ttcgattccc atgtccaaga ggcgcggcag aataccggcg gcgctgttgg tatgcagcgt
     1201 actgaagact aagtgccctg tcagggccgc ctgcactgct agctgggccg tctccttatc
     1261 gcgaatctct cccaccatca cgacattcgg gtcttgacgg agaatgctgc gcaagcccga
     1321 agcgaaggtt aggccgacat ccgggttaac ctggatctgg ttgatgccgt ccattttgta
     1381 ctccacaggg tcttccaggg tcacgatatt gatctgttcg ctcttgatct cctgaatcag
     1441 cgcgtagagc gaggtggact tgccagagcc ggtcggaccg gaggtcagga tcatgccatt
     1501 tggccgctta atacctttac ggatagctct aagcgcgcgg ccggtgtagc ccatgtcttc
     1561 cagcttgaag gaagtacccg tcttatcgag cagacgaatg accacttgct cgccccacac
     1621 cacgggcgag atagcaatac ggagatcaat tcccttgccg tcgactagaa tggtaaagtt
     1681 accgtcttgg ggtatgcggt gctcatcaat ttttaagtta gacaaaatct tgatacgaga
     1741 gataagcgcc ggttcggtgc tttttggcag gcgggtgact tcccgcagca caccgtcaat
     1801 ccggcagcga attttaagtt ctttctccag cggctcaata tggatgtcgc tggcacggtt
     1861 gcgggccgcg aattccataa ttgccgataa cgctttacta attggtgagt cttcgaccag
     1921 gttttcaatc ttcttggcct ttttccccgg ttctttggca gtttctttcg cctcagtagg
     1981 ggtcaaaccc gcgccgaagg ccgcacccat ctgggtgtcg agtcgggcgc tgtactgctt
     2041 caacacctgg cgaatgcccg cttcgctggc gacatagacc ttcagcggcc ggccaatgcg
     2101 attagacagg aagtcgacgg cctgaacatt gtcggcatct aacatggcga ccaccagccg
     2161 atgctgcatt tcaccaagtg gcactgccat gtagcgctcg gcgatctctt tgggcaggag
     2221 ttgcaggact ttcgggtcaa ccttggcgct taacagattg acgtacggca tcttggtaac
     2281 agtggcgttg gctttcgtca attgctcatc ggtgatcaag ccatttttga ctaaatagcc
     2341 gagcagtggc tgcccgacct tgccggccgc agcctttgcc gccgctagtt tgtcctcgct
     2401 caacatctcg cttttgacaa gcgtatcagt gacttgttgc tcggtggaag ccgatagaac
     2461 gctcacaaag ccgcctcctt actgtgaggg agtgccgtta aacggactgg tattgttgtt
     2521 cttgccaatt gtgatgagct tcgtcgggtc aaaagcactg ttattaaagt aatgggtgtc
     2581 cggttgcgga aaatcggtcg taatctgctg cactacttcc acttgttgct ggcggtcttt
     2641 tggcttcaca aagatggcgc tcgacgcaaa ccatgacact atggcgctaa taaaaacgat
     2701 aacccctatt aaagcaacat cttttggctt catttcacta cctgcttcgt gatatttaca
     2761 ctcttcccgg gctgatagta cgtgtgcgca gtgacggtca atgtcatgtc gttcgctccg
     2821 ccgctcaact ctatggtatc aatctgaatc ggcctaatcg aatgttccag tgcggtgatg
     2881 agctgaccga ccgaagagta actggcattg ctgaccgtaa acgaaaatgg catattgaca
     2941 gcctgtggcg aagaacttga tgtgtttttc tgctgattca actgatcgtc ggtaccggtg
     3001 atattggtaa ttttaaggct gcgatcggtc agaatctttt caatgctagt ggttagcgcc
     3061 gggaagtcgt agctcggcgg cagcgcatca agcacaattt tggcattatc gccatcattg
     3121 tcacctgagc cggcgctgag gccaccaatt acattatttg gtgtcccaac aaaagctttg
     3181 tatgaagtag tcaaattatc ataggctgaa atatttttga ccagctgctg gtgggctttt
     3241 tccttcgccg aagttacccg agattggtac ccaatctgac tccaaatcgc atgggctgcc
     3301 accaggcaga aaaccgttac aaaggaagcc acgccgacta cgcctactat ttgagcattg
     3361 gccttactaa tggctactcg cttcgtcaag atttctctat cagccattac tgaccccctg
     3421 tgctgttttt g
//
LOCUS       DATNGP010000087         4880 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_2784301, whole genome shotgun sequence.
ACCESSION   DATNGP010000087 DATNGP010000000
VERSION     DATNGP010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4880
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2784301"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            90..1604
                     /gene="secY"
                     /locus_tag="VN778_02155"
     CDS             90..1604
                     /gene="secY"
                     /locus_tag="VN778_02155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HXR49804.1"
                     /translation="MNWKIIGRSLTHKDMRGRILAVLAILLIFRILAHLPVPIGNAHT
                     LHQVLQNLFNSNNTPQLLSFINVLSGGALANFSIMIAGLGPYINASIIMQLLTKAIPQ
                     LEALHKEGEFGQKKINQYTRILTFPLAIIQSIGSIYLIRQAAQSIGGVGDITAHSTLM
                     QWVLMVTALTGGSMVLMWLGELVTEQSVGNGISLIITVGIVSRLPSSISSIIGSVSKG
                     HHWVLFGHTLPFNRNAALYSLGILIAVLLITWIVVMLNEAARNLTVNYAKRVQGNRTY
                     GGITTILPVKLITAGVVPIIFAVAFLSVPSFVGQLLTTNHSTHLAHIGTNLVTWFQTP
                     SATTFASEGWKAYIYPLTYFLLVFIFTFFYTSITFNSKEIAENLHKQGGFIEGVRGGE
                     QTERYLSKIVNRLTLFGAFSLGILAIVPIIAQVFISTSITIGGTSVLILVSVAIQTLR
                     AVESRALMVTYDQYSQPEFFNDTSALAPGAAESLAVKRPALLRLVPGRRRGSKK"
     gene            1722..1943
                     /gene="infA"
                     /locus_tag="VN778_02160"
     CDS             1722..1943
                     /gene="infA"
                     /locus_tag="VN778_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HXR49805.1"
                     /translation="MAANKEVIELTGTIVETLPGTQFRVELENGHQIIAHVAGKMRKH
                     FIRLVPGDSVTVELTPYDLTKGRITYRRG"
     gene            1987..2103
                     /gene="rpmJ"
                     /locus_tag="VN778_02165"
     CDS             1987..2103
                     /gene="rpmJ"
                     /locus_tag="VN778_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011820532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HXR49806.1"
                     /translation="MKVRASVKKISPDDKIVLRKGRLYVINKMKPKHKQRQG"
     gene            2106..2492
                     /gene="rpsM"
                     /locus_tag="VN778_02170"
     CDS             2106..2492
                     /gene="rpsM"
                     /locus_tag="VN778_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015642147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HXR49807.1"
                     /translation="MARISGVTIPAEKQVWVALTYVYGLGPKSAHDILKSAHVEETTR
                     VKNLTDAEVARIQDVINADYLVEGELQRIVSGNVKRLKDIKSYRGQRHSANLPSRGQR
                     TKTNARTRRGRKVTVGGTAKKVASKT"
     gene            2502..2930
                     /gene="rpsK"
                     /locus_tag="VN778_02175"
     CDS             2502..2930
                     /gene="rpsK"
                     /locus_tag="VN778_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004034717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HXR49808.1"
                     /translation="MAEEATTPTTTSAEPATAPKRKKTKRSVASGQVHVLATFNNTIV
                     TFTDGQGNVLTSSSAGACGFRGSKKGTAYAAQVAAERAAQAARQQYGFSKADVFIKGI
                     GLGRDAAVRVLSTLDIQVETIKDVTGVPHGGVRPRKARRI"
     gene            2930..3553
                     /gene="rpsD"
                     /locus_tag="VN778_02180"
     CDS             2930..3553
                     /gene="rpsD"
                     /locus_tag="VN778_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012475637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HXR49809.1"
                     /translation="MARDRQSIVKMSRREGYALHPKAHKALIKRSTPPGQAAGGRGVR
                     GGKASQYSLQLREKQKVKRLYGLLEKQFSNLMKEASRRQGQSGQVLLQLLEQRADNAV
                     YRAGFAPSRRAARQLVTHGHFLLNDVRIDIPSIRLKPGDSLMLRPHSSKTEYFKKLDD
                     VSPAPGEIPGWLKVNRKKVELSVTGIPSRDDAEPDINEQLIVEYYSR"
     gene            3567..4523
                     /locus_tag="VN778_02185"
     CDS             3567..4523
                     /locus_tag="VN778_02185"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF003513.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HXR49810.1"
                     /translation="MSNTIHTPGLVKVDEHSATSATFTIEPLHSGYGMTLGNSLRRVL
                     LSSISGAAVTSFRIDGVTHEFTDVTGIKEDVVDIMLNLKGIRFRVYGTESQDLRIVKQ
                     GKGVVTAKDIETNADVEIVNPEHVIATIDDAKAKLVIDLHVEVGRGYRTIEEGAAKKS
                     TDMVALDAIFSPVLRVRYKVENTRVGQATDLDRLLLSVDTDGSISPQDAFEEAAAILI
                     NQYTALAGKTRVNVHETAADNGASQDTAFGSDMLVDEPAALNTSIEDLNLSARTTNAL
                     INNDIHTIRDLFALNDAELRDLKGFGSKALDEVKEKLAELEL"
     gene            4524..>4880
                     /gene="rplQ"
                     /locus_tag="VN778_02190"
     CDS             4524..>4880
                     /gene="rplQ"
                     /locus_tag="VN778_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015642143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HXR49811.1"
                     /translation="MHRHGYQGRKLHRERDQRRALIKGLADSLVKYESIETTLPKAKE
                     VVPYVEKLITKAKRGDLHNRRQVISGLQTVASAHKLVDEIAPKLKGRASGHLRIEKTA
                     LRRGDNAQLARVSFVDD"
BASE COUNT         1265 a         1225 c         1239 g         1151 t
ORIGIN      
        1 aggagtcaag ccgggaccag cacaatcagt agacctgaag ctcctgctgt ggtttaatta
       61 aacgaagtga atcgagagac aaaccaggta tgaactggaa gattattggc agatccctga
      121 cgcacaaaga tatgcgaggg cgtattctgg cagtgctggc tattttgctc atattccgga
      181 ttttagccca tttaccagtg cccattggta acgcccatac gctccaccag gtactccaga
      241 atctttttaa ctccaacaat acgccacagc tgttgagttt tattaacgtc ctcagtggcg
      301 gggcgttggc taacttctca attatgattg ccgggctcgg gccgtacatt aacgcctcaa
      361 ttattatgca gcttttgacc aaggctattc cgcagttaga agccctgcac aaagagggag
      421 aatttggcca aaagaaaatc aaccaataca cgcgcattct gacatttccg ctggccatta
      481 tccaatccat cggttcgatt tatttgattc gtcaagccgc ccagagtatt ggcggggtag
      541 gtgatattac ggcgcacagc actcttatgc agtgggtctt gatggtcacg gccttaactg
      601 gcggttcgat ggtgcttatg tggctcggtg agctcgtgac agagcaatcc gtcggcaatg
      661 gtatttcttt aatcattacg gtgggtatcg tcagccgttt accttcaagc atctccagca
      721 tcattggcag tgtaagcaag gggcatcact gggtgttatt cggacatacc ctaccattta
      781 atagaaatgc ggcgttgtat agcctcggta tactgattgc cgtcttgctc atcacctgga
      841 ttgtggtaat gctcaatgag gcggcgcgta acctgacagt gaactacgcc aaacgggtgc
      901 agggcaaccg aacgtatggc ggtataacga ccatactccc cgtgaaactt atcacagctg
      961 gcgtggtgcc gattatcttt gcggtggcct ttttgagcgt gccgagcttt gtcggccaat
     1021 tgttgactac caatcacagc actcacctgg cgcatattgg cactaacctg gtgacgtggt
     1081 tccaaacacc ttctgccact acttttgctt ctgagggctg gaaagcatat atctatccac
     1141 tgacatattt cttgctggtg tttattttta ccttcttcta tacgagcatt acttttaact
     1201 ccaaagaaat tgccgaaaac ctacacaagc aaggcggctt tatcgaaggc gtccgcggcg
     1261 gtgagcaaac ggaacgttat ctcagtaaaa ttgtaaatcg cttgacgctg tttggggcct
     1321 tcagtctcgg catattagct attgttccta tcatagcgca ggtctttatc agcaccagca
     1381 ttactatcgg cggcacaagc gtgcttatct tggtgtcagt agcgatacaa actttacgag
     1441 ctgtagagtc tcgagctttg atggttacct acgatcagta ttcacaaccg gagttcttta
     1501 atgatacttc tgccctggct ccgggtgctg ctgaatcgct ggccgtcaag cggcccgctc
     1561 tcttgagact ggttccgggt cgtcgccgcg gttccaaaaa atagacattt gtgctactgt
     1621 aaaacgtaga tttcctccgc aggaagcaag agggtttgtt aaagaataaa aaactcttta
     1681 caaagtgttg tgagagctgc tataattgac ccttgttata tatggcggcc aacaaggaag
     1741 taatcgagct aacgggaact attgtcgaaa ccctcccggg tacgcaattt cgtgtggagc
     1801 tcgaaaatgg ccaccagatt atagctcatg tcgccggcaa gatgcggaaa catttcattc
     1861 gccttgtacc cggggatagc gtgacggtcg agttgacccc ttacgatctt acgaagggca
     1921 gaatcaccta ccgcagaggt taaatattca agcagtgtgt cattgcagta aaggagcatg
     1981 atccgcatga aagtgcgtgc tagtgtcaaa aaaattagtc ctgatgataa aattgtcctc
     2041 cgcaaaggca ggctgtatgt tatcaacaaa atgaagccta agcataagca gaggcaaggt
     2101 taagtatggc cagaatttcg ggtgtaacta ttcctgccga aaaacaagtt tgggtagctt
     2161 tgacttatgt gtacggtctt gggcctaaaa gtgctcacga catcttgaaa agcgctcatg
     2221 tcgaagaaac gacccgcgtc aagaacctga ccgatgccga agttgcccgt atccaggacg
     2281 ttatcaatgc agattattta gtcgaaggtg aattacagcg gattgtcagc ggtaacgtca
     2341 agcgcctgaa ggacatcaag tcgtaccgcg gtcaacgcca ctccgccaat ttgcctagcc
     2401 gaggccaacg aaccaaaacg aacgcccgaa cccgtcgtgg ccgcaaagtc actgtcggcg
     2461 gcaccgctaa gaaggtagcg tccaagacgt aaggattaga tatggcagaa gaagcaacca
     2521 cccctacaac taccagcgcg gaaccagcta ccgcaccaaa gcgcaagaaa actaagcgca
     2581 gtgtagccag tggccaagta cacgtgcttg caacctttaa taacaccatc gtgacgttta
     2641 ctgacgggca aggtaatgtt ctgacttcat ccagtgccgg cgcgtgtggt ttccgcggat
     2701 caaagaaagg cactgcttac gcggctcagg tagccgccga gcgagccgcc caagccgcca
     2761 ggcagcagta tggtttcagt aaggccgacg tctttattaa gggcattggt ttaggacgtg
     2821 atgccgccgt tcgagtactc agcaccttag atattcaagt cgagaccatt aaagacgtta
     2881 ctggcgtacc gcacggtggt gtgcgaccgc ggaaagcgag gaggatctaa tggctcgtga
     2941 ccgtcagtct atcgttaaaa tgagccgccg tgaaggctat gccttgcatc ctaaagctca
     3001 caaggcgctc ataaagcgct caacgccgcc aggacaagct gccggtggtc gaggtgtgcg
     3061 cggtggcaag gccagccagt actcgttgca gttgcgtgaa aagcagaagg tcaagcgctt
     3121 gtatggctta ctggagaagc aattcagcaa tcttatgaaa gaagccagtc gccgacaagg
     3181 tcaatcaggc caggtcttac tgcaattact cgagcaacgc gccgacaacg ctgtttaccg
     3241 cgccggtttt gcacctagtc ggcgagctgc ccgccagcta gttacccatg ggcacttctt
     3301 gctgaacgat gtgcgcatcg acattccatc tattcgctta aagcccggcg atagtctaat
     3361 gctacgcccc cacagctcta aaactgaata cttcaagaaa ttagatgatg tcagtcccgc
     3421 ccccggcgaa atacccggtt ggctgaaagt taatcgtaag aaagttgaac tatctgtaac
     3481 cgggatcccc tcgcgagatg atgccgagcc agatatcaat gagcagttaa tagtcgagta
     3541 ctactcacgc taaagaagga gcaagcatgt caaatactat ccacacacct ggcctcgtca
     3601 aagttgacga acacagtgcg acgagcgcca cgtttaccat cgagccgttg cacagcggtt
     3661 atggtatgac cctgggcaac agcttacgcc gcgtgctgct ttccagcatc tccggtgcag
     3721 ccgttacgtc cttcaggatc gacggtgtga cacacgaatt cactgatgtg acaggcatca
     3781 aagaagatgt tgtcgatatc atgctgaatc ttaagggtat tcgcttccgc gtgtatggga
     3841 ccgaatcgca agatttgcgg atcgttaagc aaggcaaagg cgttgtcacc gccaaagaca
     3901 ttgagaccaa tgccgacgtc gagattgtta atcctgagca cgtcattgcc actattgacg
     3961 atgccaaagc caagctggtt atcgacctcc atgtcgaagt tggacgcggc tatcgcacta
     4021 tcgaagaagg tgctgccaaa aagtcaaccg atatggtcgc cttagacgcc attttcagcc
     4081 cagttttgcg cgtgcgctac aaggtggaaa acacacgtgt tgggcaagcg acagacctgg
     4141 ataggcttct gctgagcgtt gatacagacg gttcgatctc gccacaagac gccttcgaag
     4201 aagcagctgc aatcctaatc aatcagtaca ccgcgctggc cggcaaaact cgcgtcaatg
     4261 ttcatgaaac tgcagccgac aatggcgcct cgcaagatac cgcttttggt tccgacatgt
     4321 tggttgatga accggctgcc ctcaatactt ctatcgaaga cctcaaccta tcagcccgta
     4381 ctaccaatgc cttgatcaac aatgacattc ataccatccg tgatctgttt gccctcaatg
     4441 acgctgagct gcgggaccta aaaggttttg gtagcaaggc gctcgacgaa gtcaaagaaa
     4501 agctagcgga gctggagctc taaatgcatc gtcacgggta tcaaggccgt aaactgcacc
     4561 gcgagcgcga tcagcgacgg gctcttatca aaggcctggc tgactcattg gtgaagtacg
     4621 aatcgattga gaccactttg cccaaagcaa aggaagtggt gccttatgtc gagaagctta
     4681 ttactaaagc caaacgcggt gatttgcaca atcgtcggca ggtaattagt ggtctacaga
     4741 ccgttgctag cgcacataag ctagttgatg agatagcgcc gaagctaaaa ggtcgcgcca
     4801 gcggccattt gcgtatcgaa aagacggcgc tgcgccgggg tgataacgcc cagctggctc
     4861 gtgtcagctt cgttgatgat
//
LOCUS       DATNGP010000088         2189 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_307011, whole genome shotgun sequence.
ACCESSION   DATNGP010000088 DATNGP010000000
VERSION     DATNGP010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_307011"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(34..855)
                     /locus_tag="VN778_02195"
     CDS             complement(34..855)
                     /locus_tag="VN778_02195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HXR49812.1"
                     /translation="MDKSVFRQLLVRQPASHKYDYGHVLVIGGSPGMVGAPFLSAKAA
                     LRIGAGLVTMASTPEVIDKLEKRVTEIMTLRLSSHARELTQFISERKVSVIVVGPGMK
                     AGFANAALAAIGDIDLPLIVDGGGLAALSEHHLRAQHLIVTPHLGEFERLYMGSVPPD
                     LSEAAKNFAKEKRLILVLKGHPSYISDSGQAVYQDSSGGPELATAGTGDVLSGVIAGV
                     IAQKIKPLPAVKAAVRLHGLAGKIAAELKTVPGVIASDVIEAIPAALKLVQEEEL"
     gene            959..2158
                     /locus_tag="VN778_02200"
     CDS             959..2158
                     /locus_tag="VN778_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007536545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXR49813.1"
                     /translation="MEAALRRTFASFGVRNFRLYYLGQGISLCGTWMQTIAMSWLVLE
                     LTHSGTQLGLVLAAQYLPILIFGVWGGVIADRFDKRRILYFTQSIAGLLALLLGLLVV
                     SHHIELWMVYVLAASLGFTQVVDNPSRQTFVMEMVGPANLKNAVTLNSTLVNVARVVG
                     PSIAGALIATVGVGECFLINAASFGAVLIALNLMRGSELHPAHTVAKEPGQVKAGLQY
                     VLAHPRLRSTLLMMFIIGTFAYEFPVILPLFATITLHGSATTYSAMTAAMGLGAVVGG
                     LYTAGRDVASQRELVVVAILFGLGLFAIALSPGRLVAYLIMIIVGALSVLFISMGNAT
                     LQLTSEPQMRGRVMAMWSIAFLGTTPIGGPIIGSIADHSNPRVGLAVGGLATLVAAYL
                     GIVSGRQ"
BASE COUNT          460 a          573 c          598 g          558 t
ORIGIN      
        1 tggctttgcc cctttgtcat tagctacagt atcttataat tcttcttctt ggacaagctt
       61 taaggcagcc ggaatggctt caatgacatc ggaagcgatg acgccgggaa cggttttaag
      121 ctcggcggct attttgccag ccaagccatg caggcgtact gctgctttga cggcaggcag
      181 gggtttgatt ttttgggcga tcacgccggc aatcacgccg gaaagaacat cgccagtgcc
      241 ggcggtcgcc agttcgggac ccccggaact atcttggtaa acggcttgtc cagaatcaga
      301 tatataggag gggtgacctt ttagcacgag gataagccgt ttttccttag caaagttttt
      361 cgcggcttcg cttaaatctg gcggtacaga gcccatataa agacgctcga attctcctaa
      421 atgaggcgta acaatgagat gttgggcccg cagatggtgc tcgctaaggg ccgccaggcc
      481 gcccccgtcg acaataagcg gtaaatcgat atcgccgata gcggccagag cagcgttggc
      541 gaaaccggct ttcatgcctg gcccgaccac aatgacagaa acttttcttt cacttataaa
      601 ttgtgtcaat tccctggcat gtgacgaaag gcgaagcgtc attatctcgg tgacgcgctt
      661 ttccagcttg tcgatcactt ctggcgttga tgccatggtg actaagccgg ctccaatgcg
      721 gagcgctgcc ttggcagaca ggaacggcgc accgaccatg cctggggaac cgccgatcac
      781 taagacatgg ccatagtcgt atttgtggct agccggctgg cgtactaaaa gctggcggaa
      841 cactgattta tccataagcc aattttagca ttccaagact tctgtaacag aggcgctgcc
      901 gtggcattta tcatgtaaaa agcgtataat aaagataagc attaaacaaa aggggaaaat
      961 ggaagcagcg ctgaggcgga ctttcgcgtc gttcggggtc cgcaatttcc ggttatatta
     1021 tttgggccaa ggcatttccc tgtgcggtac ctggatgcaa accattgcca tgtcgtggct
     1081 ggttttggag cttacgcatt ccggtaccca acttggtctg gtgctggccg cccaatacct
     1141 gccaatttta attttcggcg tttggggcgg cgtgattgcc gaccgcttcg ataagcggcg
     1201 gatcctctat ttcacccaaa gtatcgccgg cttgctggcc ctgctattgg ggttattggt
     1261 ggtcagccat catatagaac tatggatggt ttatgtgctg gcggccagtc tgggattcac
     1321 tcaagtcgtc gacaacccgt cgcggcagac tttcgttatg gaaatggtcg gtccagccaa
     1381 cttaaagaac gccgtcacgc ttaactccac gctggtcaat gtcgcccgtg ttgtcggccc
     1441 ttctattgcc ggagccctga ttgccaccgt cggcgttggc gagtgcttcc tgataaatgc
     1501 cgcctctttc ggcgcggtac taatagccct taatctaatg cgcggatccg aacttcatcc
     1561 ggcccatacg gttgcaaaag agcccggtca agtcaaggca ggcctacaat atgtgctggc
     1621 acatccgcgc ctgcgctcca cccttttaat gatgtttata atcggcacct tcgcctatga
     1681 gttcccggtt attttgcccc tatttgccac tatcaccttg cacggtagcg ccacgactta
     1741 ttcggcgatg acagccgcta tggggttagg cgcggtggtc ggcgggctat atacggccgg
     1801 acgggacgta gccagccaga gagagctggt tgtagtagcc atattgtttg ggctgggctt
     1861 gtttgccatc gcgctttcgc cgggacgcct tgtggcctat ttaatcatga ttatcgtggg
     1921 cgcgctttcg gtgttgttta tttcgatggg taacgccacc ctccaactaa cgagcgagcc
     1981 gcagatgcgg gggcgggtta tggcaatgtg gtcaatcgcc tttctgggca ctacgcccat
     2041 cggcggccct attattggat ctatcgccga ccactcaaat ccacgggttg gcctcgctgt
     2101 aggtggattg gctacccttg tagccgctta cctagggatc gtaagtggcc ggcaatagct
     2161 attcctaatc cgtcggcttt gtactaaaa
//
LOCUS       DATNGP010000089         4926 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_2796295, whole genome shotgun sequence.
ACCESSION   DATNGP010000089 DATNGP010000000
VERSION     DATNGP010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4926
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2796295"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..820
                     /locus_tag="VN778_02205"
     CDS             <1..820
                     /locus_tag="VN778_02205"
                     /inference="COORDINATES: protein motif:HMM:NF023413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HXR49814.1"
                     /translation="EASPSTPVVITGFKTLPEFGDEFVVVPSEKEARALAAERGGARA
                     QNGSSHGITSGELLRIINRNNETSELNIVVKADVQGSLTSVIDSLKALDTQEVAIRIV
                     GSGIGHISESDVYMASTSHAVIYGFNVSIQNNVKQLAAREKVDVRLFNIIYELIDDAR
                     QELGHLLAPEVVETELGRLVVKGVFKTTKTDVICGGDVTKGKLEVPALARVYRGDEVL
                     GEVEVTNLRRGPADAKEIFEGESCGLSFKTKHKIDLEIGDRIELFRRETVTRSL"
     gene            1021..1413
                     /locus_tag="VN778_02210"
     CDS             1021..1413
                     /locus_tag="VN778_02210"
                     /inference="COORDINATES: protein motif:HMM:NF039821.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5663 domain-containing protein"
                     /protein_id="HXR49815.1"
                     /translation="MTDTPIAPTNPTPPKGNLKIDDSLLQELGLGSLPQADKTGLLKH
                     IYETLEMRVGMRLADQMTNQQLDEFERFFNAQDDAGAFHWLETNFPNYKEIVAEEFEK
                     LKKEVAASVPQILASVQQPPAAGSAPAA"
     gene            complement(1421..2317)
                     /locus_tag="VN778_02215"
     CDS             complement(1421..2317)
                     /locus_tag="VN778_02215"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF012760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HXR49816.1"
                     /translation="MSKRLRTVFFGSGPVALSSLQLLSDRFEVEAIVTKPTTEAEMVK
                     AFPDTHVFAVQNRHELETLVSRQNFSSSFGVLIDFGIIVSQAVIDHFPLGIINSHFSR
                     LPEWRGADPITFSILSGQPQTGVSLMLLVQAMDEGPLLAQAPYNLPASITAPQLTTDL
                     IELSDALLKEVVPLYVQGIITPQNQLAATIGPKEPTYSRKLTKEDGLLDWAKPADVLE
                     REVRAYAEWPHSYTKLAGHDVIVTKAQLSEQSGAPGTIIADRKQLIVCCGSKSLEILS
                     LKPAGKPEMTAQAFLAGYGKRL"
     gene            complement(2319..2894)
                     /gene="def"
                     /locus_tag="VN778_02220"
     CDS             complement(2319..2894)
                     /gene="def"
                     /locus_tag="VN778_02220"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HXR49817.1"
                     /translation="MTSNDVISLPNPHLHEKSRKVGLITDEIRQIINDMKSATISWDQ
                     SREHEVGVALAAVQIDRLYKIVIIRNNYNNKEDLTFTAFINPVITKLEGEISEDFEGC
                     LSVKDLYGKVPRYSKIRVRALNEHGREFRVTAEGFLARIFQHEIDHTEGVLFIDHIKD
                     KTDAFFRLDAEGELVPLDYDKEIKDNRTLWS"
     gene            complement(2936..>4926)
                     /gene="priA"
                     /locus_tag="VN778_02225"
     CDS             complement(2936..>4926)
                     /gene="priA"
                     /locus_tag="VN778_02225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HXR49818.1"
                     /translation="VRFAQAIYLDKGSYYLMHYYHVWVRSDHFRGHDPLTYCLSEELA
                     PGSIVQVPLRSERVLGFVSGEVAKPAFAVKPITSRAGLPALPHQCVALAEWLQKFYAA
                     PVGVVAAQFLPNKLQAEASPDQQAQQPATALTDISLPAWTEQQKHALNTITQPDTYLL
                     HGRTGSGKTNIYLELARRSIAAGKSALILSPEIGLTSQLAREFQRPFGNRVIILHSQL
                     SASQRASAWKHILTASSPLVVIGLRSALFRPLSNLGLIVVDEAHDGAYKQEQAPYYQT
                     LRVAAQLRSLHGATLILGSATPPIGEYYLAETRKKSIIRLTELARHKTVASHEVMLVD
                     LKGRSQFSRSAHLSQALIDSMSVSLQRGEQSLLYLNRRGTARITLCDVCGWQATCPHC
                     DLPLAYHGDTYRLRCHTCGYSQSPATSCPVCGNTSVVLKSFGTKAIVDEAQKLFPDAR
                     IMRFDTDNAKDERLEHQYAQILDGQADILVGTQLLAKGLDLPKLSTLGVVLADTSLYL
                     PDYTARERTYQLLTQVLGRVGRGHVASRAIVQTFYPNGPLLAAAINDDWEQFYQTELA
                     ERKQYGFPPFTHLLKLSCRRATAASAERAAKLLKLDLERQNMPIVIDGPAPAFHEKVN
                     GKYQWQLVIKARRRSYLLAIIGNLPGNWTYDLDPSDLL"
BASE COUNT         1122 a         1204 c         1388 g         1212 t
ORIGIN      
        1 ggaagcttca ccatcaacac cagtcgttat caccggcttc aagacgttgc cagaattcgg
       61 tgatgaattc gtagtcgtgc caagcgaaaa agaagcacgc gctttggctg ctgagcgtgg
      121 tggtgcccgt gcacagaacg gtagtagtca tggtattacg agcggcgagt tattgcgcat
      181 tattaaccgc aataacgaga cgagcgagct taatattgtt gtaaaagctg atgtgcaagg
      241 ctctcttaca tctgttattg acagcttaaa ggcgctcgat acgcaagagg ttgcaatccg
      301 gattgtcggc tcaggtatcg gtcatatcag cgagagtgat gtatatatgg catcaaccag
      361 ccatgccgtg atctatggtt tcaacgtttc gatccagaac aacgtcaagc aattggcggc
      421 gcgcgagaaa gtcgatgtgc gcttgttcaa tattatctat gagctgattg atgacgcccg
      481 gcaggagctg ggacatttgc tggcacctga ggtggtagag actgagctgg gacgcttagt
      541 ggtgaaaggc gtgttcaaaa cgactaaaac agatgtgatt tgcggtggag atgtcacaaa
      601 gggcaagctt gaagtaccgg cgctggcacg cgtctaccgc ggcgatgagg tgcttggcga
      661 ggttgaagtt actaatttgc gtcgtgggcc agccgatgcc aaagagattt tcgaaggtga
      721 atcgtgtggc ctgagcttca agaccaagca caaaatagat cttgaaatcg gcgatcgaat
      781 cgagctattc cgtcgcgaaa ccgtcacccg cagcctttag catttgaggg aaaaccgtag
      841 gttgtcccta tagcttcgcg ccaagaaatc tttatatctc tagagcagga tttcttgggc
      901 tcggcatata ttctcgctgg tcgctcgaat atcaacgcgc cgacaaaatg gaatacattt
      961 tgtcgggctg ctcttttccc ttaactacaa aaagcagtaa ggagtggtac aattacccgt
     1021 atgacagata caccaatagc accaacgaat ccgacaccac caaaaggtaa tcttaagatt
     1081 gatgacagcc tgcttcagga gcttggcctg ggatctttgc cccaggcgga caagacgggc
     1141 ctgctgaaac acatctatga gacgctcgaa atgcgcgtcg gtatgcgctt ggcagatcag
     1201 atgacgaacc agcagcttga tgagtttgag cgcttcttta atgcccagga tgacgctggg
     1261 gctttccact ggctcgagac gaacttcccg aattataaag agattgtagc tgaagagttc
     1321 gaaaaactga aaaaagaagt ggctgctagt gtgccgcaaa ttcttgccag tgttcagcag
     1381 ccgccagctg ctggttccgc gccagccgcc taattggttt ctagagtcgt ttgccgtatc
     1441 cagctaggaa ggcctgagcc gtcatttcgg gtttgcccgc cggcttaagg ctgagaatct
     1501 cgagcgactt gctcccacaa cagacgatga gctgcttacg atcggcaatg atggttcctg
     1561 gcgccccaga ctgctcgcta agctgggctt tcgtgacaat aacgtcgtgc ccggcaagtt
     1621 ttgtataaga gtgcggccat tcagcatagg ctcgaacttc gcgctccaga acgtcggccg
     1681 gcttcgccca atcaagcaaa ccatcttctt tagttagttt gcgggaatag gtaggctcct
     1741 tagggccgat ggtggcggcg agctggtttt gaggcgtgat aataccttgc acatacagtg
     1801 gcaccacttc ctttagcagc gcatcgctga gctcgatcag gtcagtggtg agctgcggcg
     1861 cggttatgct ggcgggcagg ttgtagggag cttgtgctag tagcggtcct tcatccatgg
     1921 cctggacaag tagcatgagg ctgacgccag tttgtggctg gccactcaga atcgagaagg
     1981 taattggatc cgctccgcgc cattccggca ggcggctgaa gtggctattg atgataccta
     2041 agggaaagtg gtcaattacc gcttggctaa cgataattcc gaagtcgata agcaccccaa
     2101 agctactgct aaagttttgt cgactaacta acgtctctag ttcgtgtcgg ttctggacgg
     2161 cgaaaacgtg ggtgtctgga aaagccttta ccatttcggc ttccgttgtc ggcttggtga
     2221 caatggcctc tacttcgaac ctatcactca gaagttgcaa gctgctcagt gccaccggtc
     2281 cgctgccaaa gaacacggta cgcaatcgtt tgctcacgtc agctccagag tgtacggtta
     2341 tctttgattt ctttgtcgta atctagtgga accagttcgc cttcggcatc aagtctaaag
     2401 aacgcgtctg ttttgtcctt aatgtggtcg atgaacagta cgccttccgt gtggtcgatc
     2461 tcgtgctgga agatgcgagc cagaaagccc tcagcagtga ctcggaactc tcgcccatgt
     2521 tcgttgagcg cacgcacgcg aattttactg tagcgaggga ctttgccgta gaggtctttg
     2581 acactcaggc agccttcaaa atcttcgcta atttcacctt ccagtttggt gatcacgggg
     2641 ttgataaatg ccgtaaaagt caggtcctct ttgttgttat aattattacg gattatgact
     2701 attttataga ggcgatcgat ctggactgcg gcaagcgcaa caccgacttc gtgctcgcgg
     2761 ctctggtccc agctgatggt tgccgatttc atgtcgttga taatctggcg aatttcatcg
     2821 gtgataagtc caaccttacg tgatttttca tgtaaatgtg gattcggcaa agagattaca
     2881 tcattgctag tcatatttac cacagtataa cgtactaaca gatcacggcc aaatgttaca
     2941 acaggtcgct tgggtccagg tcgtaggtcc agttaccggg tagattgcct atgattgcta
     3001 gcaggtaact tcgtctacgc gctttaatta cgagctgcca ctgatattta ccgttaactt
     3061 tttcgtgaaa ggctggtgct ggcccatcga tcacaatagg catgttctgg cgttctagat
     3121 caagcttgag aagcttggcg gcacgctcgg cgctcgctgc cgtagctcgg cggcaactca
     3181 gcttcagaag gtgggtgaac ggggggaagc catactgttt acgctcggct agctcagtct
     3241 gataaaactg ttcccaatcg tcgttgattg cggccgctaa tagcgggcca ttgggataaa
     3301 atgtctgaac gatcgcgcgg ctcgccacgt gcccgcgacc aacacgacca aggacctggg
     3361 tgagtaactg atatgttcgt tctcgcgccg tgtagtccgg caggtataag ctcgtgtccg
     3421 ctagcactac cccgagagta ctgagttttg gaaggtccaa ccccttcgcg agcagctgtg
     3481 tgccaaccag aatgtcggcc tgaccgtcta ggatctgtgc gtactgatgc tccagccgtt
     3541 catctttagc attgtcggtg tcaaagcgca tgatccgtgc atcaggaaag agtttttgcg
     3601 cctcgtccac aattgccttt gtgccaaaac tctttaaaac gactgacgta ttaccacata
     3661 ccgggcacga tgtagcagga gattggctgt aaccacaggt atggcagcgc aggcggtagg
     3721 tgtcgccatg gtaggccagt ggcaagtcac agtgcgggca ggtagcctgc cagccacata
     3781 cgtcacagag tgtgatgcgc gctgtgcctc gtcggtttaa gtacagcaag ctttgctcgc
     3841 cgcgttggag gctcacgctc atggaatcga taagtgcctg tgagaggtgt gccgagcgac
     3901 tgaattggct gcggcctttg aggtcaacaa gcatgacctc atgcgatgct acggtcttgt
     3961 gccgggcaag ctcagttaag cgaataatcg atttcttcct agtttcggcc aggtagtatt
     4021 cgccaatcgg cggcgttgca gaacctagga tgagcgttgc tccgtgcagt gaccgcagct
     4081 gtgcggcgac gcggagcgtt tgataatatg gcgcctgttc ctgtttgtac gcgccatcat
     4141 gtgcctcatc gacgacgatt agcccgaggt tagatagagg tctaaagagt gcagagcgca
     4201 gtccgattac cacgagcgga gatgacgccg tcaggatgtg cttccaggcg gaagcacgct
     4261 gcgatgcgct gagctgggag tgtaggatga taacccggtt gccgaaaggc cgttgaaact
     4321 cgcgagccag ttgtgaggtg agcccaatct cgggtgacag tatgagcgcc gatttgcctg
     4381 cggcgatcga gcgccgcgcc agttcgagat agatgtttgt tttgccgcta ccagttcggc
     4441 cgtgaagtag gtacgtatca ggctgagtga tggtattcag cgcgtgcttt tgctgttcgg
     4501 tccaggcggg aagcgagatg tcggttagtg ctgtggccgg ctgctgggcc tgctgatcgg
     4561 ggcttgcttc cgcctgtagc ttattcggca agaactgtgc cgccacaacg cctactggcg
     4621 cggcgtagaa tttttgcaac cattcagcta gggcgacgca ttggtggggc agtgcgggca
     4681 gtccggctcg ggaggttatt ggttttacgg caaatgccgg ttttgccacc tcgccactaa
     4741 caaagccgag gacccgctcg ctgcgaagcg gcacctgtac gattgaacct ggagcaagtt
     4801 cttcggatag gcaatacgtc aacgggtcgt gacctctaaa gtggtcagag cgcacccaga
     4861 cgtgatagta gtgcatcaaa taataactcc ccttatccaa gtatatcgct tgcgcgaacc
     4921 tgacaa
//
LOCUS       DATNGP010000090         3338 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_3135028, whole genome shotgun sequence.
ACCESSION   DATNGP010000090 DATNGP010000000
VERSION     DATNGP010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3338
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3135028"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(255..1295)
                     /locus_tag="VN778_02230"
     CDS             complement(255..1295)
                     /locus_tag="VN778_02230"
                     /inference="COORDINATES: protein motif:HMM:NF016432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HXR49819.1"
                     /translation="MAQTEKSTPLATEQVVTDVLATIDREREREIVARRFGLFDRRET
                     LEQIGEMLGITRERVRQLEKSVVTRLKTAAEAGNLPHIADFQARVTELLQTSGEVSRV
                     SEVTAALTGDGNREEQARVAFLAQLCPQITVITEDDNFYYSVGIKKTFDEKGLKAAVG
                     KLIDAIKKLGEPKTTSDVAKAASITDQKQAAALASTSKQLATLNGRWGLVKWPMVNPK
                     NIRDKIYVILSENGRHMHFNEIAEAIKDSSFKRKDVTTQAIHNELIKDKRFVLIGRGI
                     YALKEWGYEKGTVADIITKVLQDAGEPLHRDEIVKRVLKSRFVKETTILLNLQGKPQF
                     KRVAKATYTLAE"
     gene            complement(1493..>3338)
                     /locus_tag="VN778_02235"
     CDS             complement(1493..>3338)
                     /locus_tag="VN778_02235"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HXR49820.1"
                     /translation="LSPVLWKKVRTGLSAGRVQSVAVRLIVEREREIRDFQAESTFKI
                     TAIFMADGQEIPAELETKLPDAQAARAWLEHAIDAAFKVENIETKPGSRNPGAPFTTS
                     TLQQEAARRLGFSVRQTMTLAQRLYESGHITYMRTDSTTLSSFAIQTAEKYITDHYGV
                     NYHTRRQYKTKNQSAQEAHEAIRPTDFSKTALGDDEQQKKLYTLIWQRALASQMSAAD
                     IERTEVKIAVSTQAERFIAKGEILRFDGFMKVYGGTKDDVLLPPLTVGQGLNLATMTA
                     TETFSRPPARYSEAALVRKLEDLGIGRPSTYAPTISTIQTRGYIEKKDLEGEQREITT
                     LQLNNTQITDFKTTVLTGADRGKLVPTPIAEVTTDFLVKYFPNVTDYSFTARVEEDFD
                     EIADGKLAWAVMIDRFYQDFHPLVEKSEDIPRNEVSQARELGQDPGSGEAIYARFGRY
                     GPMLLKGDTEDETKKPVFAPLPAGTTIDTVTLEQALEMFKLPRLAGKTEDGKDIKASI
                     GRFGPYIQVDKTFVSIKPLDPHNITEGEARKLYAEKLAKDAAKHIKEFTGGIKILNGP
                     YGPYVTDGKKNARIDKSEDPAKLTEAAAKAILEAAPAKKRGFRRKAKS"
BASE COUNT          660 a          838 c          921 g          919 t
ORIGIN      
        1 ccatgactca gctttgaagt ctgcgtattg gttgcggtgt agacgcaatg ataatctatg
       61 ggtgtgcccg attgaggaga tgcatcaatg aggtgtaata cccttacggc taactcctct
      121 ctctcagcct ctaagaccat cggtccattt actgctagca gtactcgtac ctgtcgatga
      181 aagaacccgg cgacagactc accggagggc ggtccttgtt ctagtctatg agccatgagt
      241 tattggtgtt aaatttattc ggcgagggtg taggtagcct tggcgacgcg cttaaactgc
      301 ggcttgcctt gaaggttcag gaggatggtg gtttctttga caaagcggct cttgagcacg
      361 cgcttgacga tttcgtcgcg gtgaaggggt tccccggcat cctgcaggac cttagtgatg
      421 atgtcagcga cggtgccctt ctcgtagccc cattctttga gggcgtagat gccacggccg
      481 atcaggacga aacgcttgtc tttgatgagc tcgttgtgga tcgcctgggt ggtaacatct
      541 ttacgcttaa agctgctgtc tttgatggct tccgcgattt cgttgaagtg catgtgtcgg
      601 ccattttcgc tgaggataac gtagatcttg tcacggatat tcttaggatt aaccatcggc
      661 catttaacga ggccccagcg gccgttcagg gtagccaact gcttcgaagt gctagctagg
      721 gcggcagctt gcttttggtc agttatactg gcggctttgg caacgtcgct ggtagtcttt
      781 ggttcgccca attttttgat ggcgtcaata agtttgccaa cggcagcctt aagacccttt
      841 tcatcaaatg tctttttgat gccgacactg tagtagaaat tatcatcttc cgttatgacg
      901 gtgatttgcg ggcagagctg ggccagaaaa gctaccctgg cctgctcttc gcggttgccg
      961 tcgccggtga gcgcagcagt cacttcactc acgcgagaca cctctccgct agtctggaga
     1021 agttccgtca cgcgggcttg gaagtcggca atatggggca aattccctgc ttcggcggcg
     1081 gtcttgaggc gagttacgac tgatttttcg agctggcgaa cccgttcgcg ggtgatgcca
     1141 agcatttcgc cgatctgctc gagcgtctcc cggcgatcaa atagcccaaa gcgccgggca
     1201 acgatttctc gctcccgttc tcggtcgata gtagccaata catcagttac cacctgttcg
     1261 gtggctaacg gcgtggattt ttcagtttgg gccatagata tgatttccct actccgtata
     1321 ttaatactat tataaaacaa taatttttaa aagtcaacac ttttatcttc taaagctatt
     1381 gtagcacatt cctgacagcc ttgcgcaaac gatggtgtaa agatcttatc tgaacacttg
     1441 ttttgctacg aggcaattat tcagtaaacc agagatatcc acagcctact ggctaagatt
     1501 tggccttccg gcgaaagcct cgttttttag ctggtgccgc ctctaaaata gccttcgccg
     1561 ctgcttcggt gagcttggct gggtcttcag acttatcaat acgggcattt ttcttgccat
     1621 cggtgacata cggtccataa gggccgttga ggattttgat tccgcccgtg aactccttga
     1681 tgtgcttcgc ggcgtccttc gccagcttct cagcgtataa tttacgagct tcaccttcgg
     1741 taatgttgtg gggatcaagc ggcttgatag atacgaacgt tttgtcgact tggatatacg
     1801 ggccgaagcg accaatgctg gcttttatat ctttcccgtc ctccgtcttg cctgccaacc
     1861 gcggtaattt gaacatttcc agcgcttgct cgagggttac agtatcgatg gtagtgccgg
     1921 ccggcaatgg ggcaaagact ggctttttgg tctcgtcctc ggtgtcgccc ttgagaagca
     1981 taggaccgta gcggccaaaa cgggcgtaaa tcgcttcgcc gctccccgga tcttggccca
     2041 gctctcgggc ctgcgacact tcatttctgg ggatgtcttc agacttttcg accagtgggt
     2101 ggaaatcctg ataaaaacgg tcgatcatga ccgcccacgc caacttgcca tcagcgattt
     2161 catcaaagtc ttcttcgact cgcgccgtaa agctgtaatc agtcacgttc ggaaagtact
     2221 tgaccagaaa atcagttgtc acttctgcga tcggtgtcgg cactagtttg cccctgtcag
     2281 caccggtcaa aacagtagtt ttgaagtcgg tgatttgggt attgttcagc tggagagtgg
     2341 tgatttctcg ttgctcgcct tccaggtctt tcttctcgat atagccacgc gtttggatgg
     2401 tggaaatagt tggtgcatag gtactgggcc ggccaatgcc gagatcttcg agtttgcgca
     2461 ccagcgcagc ttccgaatag cgggccgggg ggcgactgaa ggtttcagtg gcggtcatgg
     2521 tggctaggtt caggccctgc ccgactgtaa gtggcggtaa aagcacgtcg tccttggtgc
     2581 cgccgtatac tttcataaag ccgtcaaaac gcagaatttc ccccttggca ataaagcgtt
     2641 ctgcttgcgt gctgactgcg atctttactt cggtacgttc gatgtcggcg gcgctcattt
     2701 ggctggctaa ggcgcgctgc cagatcaatg tgtagagctt cttttgctgt tcgtcatcgc
     2761 cgagagccgt tttgctaaaa tcggtgggcc ggatagcctc atgggcttcc tgggcgcttt
     2821 gatttttggt tttatactga cggcgcgtgt gatagttgac gccgtaatgg tcggtaatgt
     2881 acttctcggc agtctgtata gcgaaactac tgagcgtcgt gctgtcggtc cgcatatagg
     2941 tgatgtggcc gctttcgtag aggcgctgag cgagcgtcat ggtttggcgg acgctaaagc
     3001 cgaggcggcg agccgcttct tgctgcaggg tgctcgtcgt gaacggtgcg ccgggattgc
     3061 ggctgccggg cttggtttcg atgttttcaa ccttgaaagc ggcatctata gcgtgctcga
     3121 gccaggccct ggccgcttgg gcatcaggca gtttagtttc aagctcagcc gggatttcct
     3181 gaccatcggc catgaagata gcagtgatct taaaagtgct ttcggcttgg aagtcgcgga
     3241 tttcccgctc tctttcgaca atcaagcgga ccgcgaccga ctgcacacga ccggcactga
     3301 gcccggtacg taccttcttc cagagtactg gcgagagt
//
LOCUS       DATNGP010000091         4229 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_2754952, whole genome shotgun sequence.
ACCESSION   DATNGP010000091 DATNGP010000000
VERSION     DATNGP010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4229
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2754952"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..276
                     /locus_tag="VN778_02240"
     CDS             <1..276
                     /locus_tag="VN778_02240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49821.1"
                     /translation="NFMAVHLSEIAGGQTYSQVSNAAMADPTNAKLQAEKQTLFQGET
                     LRGLLLGDGYAYWTFGQIALTAALVTLAGAGVMAVLVLLGLRHMSRT"
     gene            324..1469
                     /locus_tag="VN778_02245"
     CDS             324..1469
                     /locus_tag="VN778_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005094891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHYH protein"
                     /protein_id="HXR49822.1"
                     /translation="MDENQISPITPDIPVTATPPQSVAPPPKSRKKLWLLMAVIVLIS
                     AAAAFWLLNHHTHTQQPVSQTSHTKTSTAPSATLATTQGLKLDPNKNYGNKYANGLLP
                     VGDGKYTTTSAKQGYVYACSTYAQSLSSGQGGAGTRGPWFTDNNTVYDINKEVHVAGS
                     VAWHGSFSDIINSDNRIITTNDLPNHVTGIFPIAATDPAYAYDRNPNSLAAQNLTYTL
                     SANPIYGSPQCMGGEAGVMLTGVALFNAFDAGGRDAGAWEVQDQCGGHPQRSHEYHYH
                     TLSSCISDTSVSTVIGFALDGFPITGPKVSQSNILTTADLDECHGIVSTVNLDGKKVT
                     TYHYVMTQDFPYSVSCFRAKPSPTGPSAGGQQTGPPANQLGPPPRRQ"
     gene            1600..2613
                     /gene="carA"
                     /locus_tag="VN778_02250"
     CDS             1600..2613
                     /gene="carA"
                     /locus_tag="VN778_02250"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HXR49823.1"
                     /translation="MSLSDGTQFAGTMPAWQTGPYVGEVVFNTGMTGYVESLTDPSYA
                     GQILVFTYPLIGNYGVQLDSAESHKIQVSGVIVSEAVLDYSHSDAELALPAWLASQNI
                     PLLTEVDTRALTKHLRISGTMGGAIGTKTTKPGAIKLTSHFVSIDKPVVYQANKKKCV
                     ILVDCGAKENILRSMLALPVQVKRVPHDYDYTGEDYDGVLLSNGPGDPIDYQATITHV
                     RHALGKGKPVFGICLGSQIMGLAAGAQTYKLKFGHRGHNQPCLETGTEHGYITSQNHG
                     YAVDERTLPKDWIVSFRNLNDNSVEGIEHKAKPFFSVQFHPEACPGPTDTSWLFERFY
                     KTL"
     gene            2610..>4229
                     /gene="carB"
                     /locus_tag="VN778_02255"
     CDS             2610..>4229
                     /gene="carB"
                     /locus_tag="VN778_02255"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HXR49824.1"
                     /translation="MSSDHPPQKIKKVVVLGSGGLRIGQAGEFDYSGSQAIKALKEEG
                     IYTVLINPNIATVQTDEDMADRVYFQPLNIVTVTKILQREKPDAVMLSFGGQTALNLG
                     LQLHEEGILNKLSIQVLGTPVSVIKITEDRELFKNALKEIGTKTARSVAAYSLKEALS
                     AAHKIGYPIMMRSGFSLGGLGSGRINNDDDLTHRVQEVLKAVPQVLIEEYLAGWKEIE
                     YEVVRDRDGNTITVCNMENLDPMGIHTGESIVVAPSQTLTNEEYHHLREVAIQTINHL
                     GIVGECNIQYALNPANGDYRVIEVNARLSRSSALASKATGYPLAFVASKLMLGYKLLE
                     LKNAVTGKTSAFYEPALDYLAVKMPRWDLAKLKSSDHHIGSEMKSVGEVMALGRSFPE
                     ALQKATRMLNIGADGLSRHPYEFPDPLPEIEHATDRRLFAIYEALKRGQSVEHIHKLS
                     RIDKWFLDHIAAILQTEKRLATEPLDTHLLRSAKRLGFSDKAIASLKGQDEAEIRAHR
                     LKRGILPAIKQIDTLAGEFDAETNYLYVTYNG"
BASE COUNT         1070 a         1205 c         1029 g          925 t
ORIGIN      
        1 aacttcatgg ccgtacacct tagtgaaata gcaggtggtc aaacgtactc acaggtaagc
       61 aatgcggcta tggccgatcc gaccaacgct aagctgcagg ccgaaaagca gaccctgttc
      121 cagggcgaaa ccttgcgcgg attactgctc ggcgacggtt acgcttactg gacattcggc
      181 caaattgctc tgacggcggc gctcgtcacc ttagccgggg ctggcgtgat ggctgttctg
      241 gtactgctcg ggctgcggca catgtcccgc acttagcttc gttgagattg cctccgacat
      301 agtggttatg ttataaacca cctatggacg aaaaccaaat ttctcctatt acaccggaca
      361 tacccgttac tgctacccct ccacaatcgg tcgctcctcc gccaaagtcg cggaagaaac
      421 tctggctgct catggccgtt atcgtgctga ttagtgccgc tgccgctttc tggctgctca
      481 accaccacac gcacactcag caaccagtaa gccaaacatc gcacaccaaa acttcgactg
      541 ctccttcagc tactctcgcc accacgcagg gcttgaaact tgatcccaat aaaaactatg
      601 gtaataaata cgccaacggt cttttacccg ttggcgacgg taaatacacg accaccagtg
      661 ctaagcaagg ttacgtttat gcttgcagca catatgccca aagtttgagt tccggccaag
      721 gcggtgccgg cacgcgcggc ccttggttta ctgacaacaa caccgtatat gatatcaaca
      781 aggaggtgca tgtagctggc agcgtcgcct ggcatggtag ctttagcgac atcataaata
      841 gcgataaccg gatcattacc accaatgacc tgccgaacca tgtcacaggt atctttccta
      901 tcgcggccac cgatccggca tatgcctacg accggaaccc gaattcgcta gccgctcaaa
      961 acttgaccta tactttgtcg gctaacccca tttacggttc accgcagtgc atgggtggcg
     1021 aggcgggtgt aatgctgact ggagttgctc tcttcaatgc tttcgatgcc ggcggccgtg
     1081 atgctggcgc ctgggaagtt caagaccaat gcggtggcca tcctcagaga agccacgaat
     1141 atcactatca cactttgagc agctgcatct ccgacacctc ggtttcgacg gtcatcggct
     1201 tcgcgcttga cggttttccc atcaccggac cgaaagtcag ccaaagcaat atcctgacca
     1261 ctgcagacct tgacgaatgc catggtatcg tcagcactgt caacttggac ggcaaaaagg
     1321 tgacgaccta ccattacgtc atgactcaag atttcccgta ctccgttagc tgcttccgtg
     1381 ccaaaccatc ccccaccggc ccttccgccg gcggccaaca aacaggccct ccagcgaatc
     1441 aacttggtcc gccgccacgc cggcagtaac ttgcataaag cttgtccgtc tggtagattc
     1501 aatgggagta tgcggagttc gccatcgatc acaaacagct tgaaaaaatc gacaatcgct
     1561 ccaccccgga gcgatttttt taaaaccaaa actggatatt tgagccttag cgatggcaca
     1621 caatttgcag gtacaatgcc ggcctggcaa accggccctt atgtcggcga agtagtcttc
     1681 aataccggca tgaccggtta cgtggaatcg ctgaccgacc cttcctatgc cgggcaaatc
     1741 ttggtcttta cttatccgct gatcggcaat tacggtgtgc agctggatag cgctgaatcc
     1801 cacaaaattc aggtaagcgg cgtcatagtt tcggaagccg tcctggacta cagccatagc
     1861 gatgccgaac ttgcactgcc agcttggctg gcctcccaaa acataccttt gctcacggag
     1921 gtagacaccc gagcgctaac caaacatctc cggatcagcg gtactatggg cggcgctatc
     1981 ggcaccaaga caaccaagcc aggagcgatt aagctcactt cacattttgt ttctatcgac
     2041 aaaccagttg tgtaccaagc caataaaaag aagtgtgtca tcctggtcga ttgcggcgcc
     2101 aaggaaaata ttttgcgttc tatgctcgcg ctgccggtgc aagtcaaacg tgtaccccac
     2161 gattatgatt acaccggcga agactacgac ggtgtgctgc tatcaaacgg tcctggggac
     2221 ccgatagact atcaagcgac gatcacgcac gtccgccatg cgcttggcaa aggcaagccg
     2281 gtctttggca tctgcttggg gagccagatt atggggctgg cggctggtgc tcaaacctac
     2341 aaattgaagt ttgggcaccg cggacacaac cagccttgcc tggaaactgg taccgaacac
     2401 ggttacatca ccagccaaaa tcacggttat gctgttgacg agcgcactct acccaaagat
     2461 tggattgttt cgttccgcaa tctcaatgac aattccgtgg aaggcatcga gcataaggcc
     2521 aaaccattct tttcggtaca gttccatccc gaagcctgtc cgggcccgac cgataccagc
     2581 tggctgtttg aaaggtttta taagacttta tgagttccga ccacccacca caaaaaataa
     2641 aaaaagtggt ggttctcggc tccggcggcc tgcgcatcgg ccaagccgga gagtttgact
     2701 attccggctc acaagccatc aaggccttaa aagaagaagg tatttacact gtgcttatca
     2761 accccaatat cgccactgtg caaaccgatg aggacatggc cgaccgggtc tattttcagc
     2821 cgcttaatat cgtcactgtt accaaaatcc tgcagcgcga aaagccggat gctgtcatgc
     2881 tcagcttcgg cggccaaaca gcgcttaatc tagggctgca actgcatgaa gaaggaatac
     2941 ttaacaagtt aagcattcaa gttttgggta cacccgtatc tgttattaag atcacggaag
     3001 accgcgagct atttaaaaat gctctgaagg aaatcggcac caagaccgcc cgcagtgttg
     3061 ccgcctacag cctcaaagaa gctctgagcg ctgcccataa aattggctat cctatcatga
     3121 tgcgatccgg cttcagcctc ggcgggctgg gctcaggacg gattaacaac gacgacgatc
     3181 taacacaccg ggttcaagaa gttttgaagg ccgtaccgca agtcttaatc gaagaatact
     3241 tggccggctg gaaagaaatt gagtatgaag tcgtccggga tcgggatggc aacacgatca
     3301 ccgtctgcaa catggaaaat ttagacccca tgggtatcca taccggcgag tcaatcgtgg
     3361 tcgccccgtc acaaaccctg accaacgaag agtatcatca cctgcgagag gtcgctatcc
     3421 agaccatcaa tcatctcggc attgtcggcg agtgcaacat ccagtacgcg cttaatccgg
     3481 cgaacggcga ttaccgagtc attgaggtca acgcccgttt gtcgcgctcc agcgcgctgg
     3541 cctctaaagc taccggctat cccctggcat tcgtagcatc caagctgatg ctgggctaca
     3601 agctactaga attgaaaaat gctgtgacgg gcaaaacgag tgccttttac gagccggcgc
     3661 ttgattacct ggcagtcaaa atgccgcgtt gggatctcgc caagcttaaa tccagcgatc
     3721 atcatatcgg cagtgagatg aaaagtgtcg gcgaagtcat ggcgcttggg cgctctttcc
     3781 cggaagcttt gcagaaagcc acgcgcatgc tgaatatcgg cgcggacggc ctgagccgtc
     3841 atccgtacga gttccctgac ccgctgccgg aaattgaaca cgccacagac cgccgactct
     3901 tcgctatcta tgaagccctg aaacgtgggc aaagcgtcga gcacatccat aagctatcga
     3961 gaattgacaa atggttttta gatcacatcg ccgctatttt gcagaccgaa aagcggctag
     4021 cgactgagcc cctcgatacc cacctgttac gctcggccaa acggttgggc ttttctgaca
     4081 aagctatcgc cagcctgaaa ggtcaagacg aagccgaaat tcgagcacac cgccttaagc
     4141 gcggcatcct gcccgctatt aagcaaatcg ataccctagc cggtgaattc gatgccgaaa
     4201 ctaactattt gtatgtcacc tataacggc
//
LOCUS       DATNGP010000092         2129 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_291495, whole genome shotgun sequence.
ACCESSION   DATNGP010000092 DATNGP010000000
VERSION     DATNGP010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2129
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291495"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            43..234
                     /locus_tag="VN778_02260"
     CDS             43..234
                     /locus_tag="VN778_02260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49825.1"
                     /translation="MADLVLQATELRKSFTTRRGQKAVTAVDGVDLEIKKGRSLRLFR
                     PQRCWQDHNLAHADDLATD"
     gene            203..922
                     /locus_tag="VN778_02265"
     CDS             203..922
                     /locus_tag="VN778_02265"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXR49826.1"
                     /translation="MRMLTTLLPIDSGTATVAGFDVTSNPQAVRERTGYVSQLGGADN
                     LATGREDLLLQAQLYGMSFRDASKRSDELAKALDLKDFIDRRVTTYSGGQRRRLDIAL
                     GIMHRPEILFLDEPTTGLDPQNRANLWDQVRKLRDNGTTIFLTTHYLEEADALSDRLA
                     IMDHGEIVATGTPRELKKQISGDAVVIKPRHDGATVEKVRQFLGEQPFVRDARPEGET
                     IRLYVDDGTAMLPKIFDLLKA"
     gene            1039..1800
                     /locus_tag="VN778_02270"
     CDS             1039..1800
                     /locus_tag="VN778_02270"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXR49827.1"
                     /translation="MKLYHDITLLYKRTLMQTLRNMIWVFVGVSTPILYLALFTPLLK
                     KLANGPGFTTGHVLDIFLPGILALMAFSGGISQGFGTIFEIQSGLVERLRVTPASRLA
                     LLLSPILAAITFNYCFMVLVVVIGMALGFHLHLLGLLGVFVLLGLVMAAFAAFSIALA
                     LLTKEISSFAAPVNGLNLPLLLLSGVLLPLTLAPGWMVVLAHFNPLYYAVEGARTMAT
                     GSFSGAAVWQSFAVMIPLTALLLWWATRVYRKAVA"
     gene            complement(1744..>2129)
                     /locus_tag="VN778_02275"
     CDS             complement(1744..>2129)
                     /locus_tag="VN778_02275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49828.1"
                     /translation="QLERLRAWHGQRQLYATSAGAVTLLAPILFGYFVYVAIRLHQPI
                     FFILCLIVLTIVLLLTIWGDEHLRPLQKLTYSVSIPITYGLFCLFALLLPMALVAETV
                     ASIEWHDLSYRLAVDPSSPPQEERS"
BASE COUNT          489 a          562 c          559 g          519 t
ORIGIN      
        1 tggcttttac acctggcaaa cattataaaa taaggagaat gtatggctga tctggtactg
       61 caagcaactg agctacgcaa gtccttcacc acccgccgcg gtcaaaaggc cgttacagcg
      121 gtcgatggtg ttgacctgga aataaaaaaa gggcgaagtc ttcggctttt taggccccaa
      181 cggtgctggc aagaccacaa ccttgcgcat gctgacgacc ttgctaccga ttgatagtgg
      241 cactgcgacg gtggctggct ttgatgttac atcgaatccg caggctgtgc gtgaacgcac
      301 gggctatgtc agccaactgg gcggcgccga taacttggcg accggtcgtg aggatttact
      361 attgcaagcc cagttatatg gcatgagttt tcgcgatgct agtaaacgct ccgacgagtt
      421 ggcgaaagcc ctcgatctga aagattttat cgaccggcgc gtgacaacct actcgggcgg
      481 ccagcgccgg cggctcgaca tcgccttagg tatcatgcat cgtccggaaa ttttgtttct
      541 cgatgagccg accactggct tagaccctca gaatcgggct aatctttggg atcaagttcg
      601 caaactgcgc gacaacggta ccacaatctt tttgaccacg cattatttgg aagaagcgga
      661 cgcgctcagc gaccgactgg cgattatgga tcacggcgaa atcgtcgcca ccggcacgcc
      721 acgcgagctg aaaaagcaga tctctggcga cgcggtcgtc attaaacctc gtcacgacgg
      781 cgcgaccgtt gaaaaggtcc ggcaatttct cggcgagcaa ccatttgtac gtgatgcccg
      841 gcccgaaggt gagactattc gtttatacgt tgatgacggc acagccatgc tgcccaaaat
      901 tttcgatctg ctgaaagcct aaaaagtcga actagaaacg gtctctttgt cgcagccatc
      961 gctcgacgat gtgtttttgc agcaaaccgg tcgcagtttg cgcgatgccc gagtcgccga
     1021 agaaggaagg aaataaatat gaaactctat cacgacatta cactgcttta caaacgcaca
     1081 ctaatgcaga ctttacgcaa catgatttgg gtgttcgttg gagtatcgac acccatcctc
     1141 tatttagcac tcttcacgcc actgctgaaa aaactagcca acggaccggg ctttacgact
     1201 ggccacgtgc tcgacatttt tctaccaggc atcttagcct tgatggcctt cagcggtggt
     1261 atttcccaag gtttcggcac gatttttgaa atccagagcg gcctggtgga acgcttgcgg
     1321 gtgacgccgg ccagtcgcct ggcgctgctg ctcagtccga ttttggctgc cataacattt
     1381 aattactgct ttatggtact tgtggtcgtc attggtatgg cgttgggctt tcacttgcac
     1441 cttttgggtt tgctcggcgt gtttgtgctc ttaggtctcg tgatggccgc ctttgctgcc
     1501 ttttcgattg cgctggccct cctgacgaaa gaaatcagta gctttgcagc gccagtaaac
     1561 ggcttgaact tgccgctgtt gctcctttcg ggcgtactct tgccgctgac cctggcaccc
     1621 ggctggatgg tggtactggc tcatttcaat ccgttgtact acgctgtcga aggtgcccgc
     1681 accatggcaa ccggcagttt ttccggagca gcagtctggc aaagtttcgc cgttatgatt
     1741 cctctaactg cgctcctctt gtggtgggct actcgggtct accgcaaggc ggtagcttaa
     1801 atcatgccat tcgatcgagg caactgtttc ggcaaccagc gccatcggta ataaaagcgc
     1861 aaagagacaa aatagcccgt acgtaatggg gatgctgacg ctgtacgtca gcttttgtag
     1921 tggcctgagg tgctcatctc cccaaattgt cagcagcagc acgattgtca aaacaatcag
     1981 acacaagatg aaaaagattg gctgatgcaa tctgatcgcc acatacacaa aataaccaaa
     2041 cagtatgggt gccagtaaag tgactgcacc ggccgaggtc gcgtacagtt gtcgctgccc
     2101 atgccaagcc ctgaggcgtt ctagctgta
//
LOCUS       DATNGP010000093         3898 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_3447371, whole genome shotgun sequence.
ACCESSION   DATNGP010000093 DATNGP010000000
VERSION     DATNGP010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3898
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3447371"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..250)
                     /locus_tag="VN778_02280"
     CDS             complement(<1..250)
                     /locus_tag="VN778_02280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49829.1"
                     /translation="MTNSHQPILYDKLIRLTHVYLGPAADRFISRQIQNHLNKEPRDL
                     TREDLLQLIDWIRVTVSLLTDDNEVVEEYIAQVRNLAMA"
     gene            complement(254..1192)
                     /locus_tag="VN778_02285"
     CDS             complement(254..1192)
                     /locus_tag="VN778_02285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49830.1"
                     /translation="MATVRNHNYYMMKAILSFIVLSVIYALLVLLLPANKETMHMYHL
                     TPEAYHVLLSALTMPSLTVWLVAFLAYGKLYEYVDLINDSTESIGFARMARGIKWLAW
                     SLPLSSIVSLVAFAIMNTSSGCHTIGIVANNYLTLILPLIGYSLIGSGGRSLMAQAKL
                     SFSVSDAQSIMLSFIGGGVLYCYLTFRQLSSIRLSSTHNPYALPVWLVVVSIIIPYLY
                     AWFVGVLAALEINTYSKRVRGLLYRQPLRLLVMGLTIVIASSIALQFLGTVVPATGHI
                     LLNYHLTFRFLFRAASGLGFLLITLGVLRLKKIEEV"
     gene            1310..2272
                     /locus_tag="VN778_02290"
     CDS             1310..2272
                     /locus_tag="VN778_02290"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HXR49831.1"
                     /translation="MTDLWHTVYVKRRQALYLLAILLTLYVILPQLGSFRASKAQLAH
                     IRLEYVVLAVIAMGLTYTAAAGTYGFLALNRLKYRDTVLVQMASNFVNRLLPAGIGAL
                     GANYAYLRRSKHSSSQAVTVVSINNILGIIGHLAIVALAVGFFRKQLQTVQLGHVRHE
                     LQWLVAGLIILLPLVLFLARPHGRLRQVGHEISVQLGHYRRRPERLLGALLTSMTLTL
                     CNVLSLWYCAQALHAHLSFVTILLIFSLGVGVGTVTPSPGGLGGVEAGLVAGFVAYHL
                     TGAQALAVALLYRLITYWLPLIVGAFVFVLCVRRELFSRQQLTH"
     gene            complement(2269..2928)
                     /locus_tag="VN778_02295"
     CDS             complement(2269..2928)
                     /locus_tag="VN778_02295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49832.1"
                     /translation="MAILGNKYREHDPVWHVQTTMLVAIILQLLLPDRYVLGSRYLLL
                     VVEAVLLIALSFTTPKERIFRSLSRRINVFALIFLTGVANGYSLIEVARKLLQSGRVH
                     NGRDLVLTAVNIYLTNIVIFALWYWEMDGGGPGRRAQAARHEQDFLFPQNQNESYKHP
                     KWRATFIDYMYVSSTNAMAFSPTDTMPLTRRAKLMMLVQATISLVAIALVAARAVNIL
                     S"
     gene            complement(2971..3708)
                     /locus_tag="VN778_02300"
     CDS             complement(2971..3708)
                     /locus_tag="VN778_02300"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXR49833.1"
                     /translation="MYQLWLRQVRRYWRSGSRLLGSLGQPVLLLLALGYGIGAIYRRA
                     GEGNYLNFLVPGIITQTVLLSGVFWGMIILMDKRFGFLAELLVSPVKRSKILLGSALG
                     GATVSIIQGTLVFILSLIFGFRPHNWFLVPPAFLVLVLMCLALACFGAGIASMLDDFQ
                     GFQGINNLLILPMFFLSSALYPLNQIPIGLKIISTINPVSYMVDALRHLLSNQAHFNF
                     SVDIGVMAATFVLCLTFAVNRFNRIQM"
     gene            complement(3717..>3898)
                     /locus_tag="VN778_02305"
     CDS             complement(3717..>3898)
                     /locus_tag="VN778_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXR49834.1"
                     /translation="IIDHGKIVARGTTAELTKQTATKTLEQAYLKLTGTTVRDESGNN
                     NGLNAQMARRNMRLR"
BASE COUNT         1076 a          928 c          944 g          950 t
ORIGIN      
        1 gagccattgc cagattacgc acttgagcaa tatactcttc aaccacttcg ttgtcatcag
       61 ttagaagcga gactgtaaca cgtatccagt cgataagttg aagtaaatcc tcgcgagtta
      121 gatcccttgg ttctttgtta aggtggttct gaatctgacg agagataaat cggtccgcag
      181 caggccccag atagacatga gttagtcgaa taagcttgtc gtagagaata ggctggtgcg
      241 aattggtcac ggcttagacc tcctcgatct tttttagccg taaaactcca agtgtaatca
      301 ataaaaagcc aaggccagaa gctgcccgga aaagaaacct aaatgtcaga tgatagttaa
      361 gcaagatatg ccccgttgcc ggcacgactg tacctaaaaa ttgcaaagcg attgaactgg
      421 cgataacaat cgtcaggccc atcacgagca atcgcaaagg ctggcggtac aatagcccac
      481 gaacccgctt gctataggta tttatttcta aagcagctag tactccaacg aaccaagcgt
      541 acaaatatgg aatgatgata ctgactacta ccaaccagac cggcaaagcg taaggattat
      601 gggttgagga aagtcttatc gaactcagct gccggaatgt taagtagcag tacaacaccc
      661 caccgcctat aaatgacaac ataatacttt gagcatcact aacactaaag ctcagtttgg
      721 cttgagccat aagtgaccga ccgcctgaac caattaagct ataacctatg agtggcagta
      781 tcaaggtgag gtaattatta gcaacaatgc ctatcgtgtg gcagcccgaa gaggtgttca
      841 taatagcgaa tgcaacaagt gagacaattg aagataatgg taggctccag gccagccatt
      901 taataccccg agccatccgc gcaaaaccaa tgctttcagt agaatcattg atgaggtcaa
      961 cgtattcata taatttaccg taagctaaga aggcaactag ccaaacggtc aaagacggca
     1021 tagtgagggc tgatagtagc acatgatagg cttcaggcgt caggtggtac atgtgcattg
     1081 tttccttgtt ggcaggcaat aacaaaacga ggagtgcata tatcacactc agtactatga
     1141 agctcaagat ggccttcatc atgtaatagt tgtggtttct gacggttgcc ataatactga
     1201 cataattata caagaatttg cataaaaccc ttttgtgtcc ctagacaaag cgcggatcct
     1261 cgagcttctc tcttgaaact gttataaaac tcagcgataa tgaccataca tgactgacct
     1321 ctggcatacc gtttatgtga agcgccgtca agctctctat ctgctcgcaa tattactgac
     1381 gctctatgtc attttgcctc agttgggcag ctttcgggca agcaaagcgc agttggcgca
     1441 tatccgtctg gaatatgtgg tactggcggt tattgctatg ggtctgacgt atacggcggc
     1501 ggcgggaacg tacggctttt tggcgttaaa ccgtttgaaa tacagagaca cagtcttagt
     1561 tcaaatggct agcaattttg tcaatcgctt attgccggcc ggtattggtg cgctaggcgc
     1621 taattacgca tatttacgaa gatcaaaaca tagctcgagt caggccgtga cggtagtgtc
     1681 aatcaataat atcttaggta ttatcgggca tcttgcaatt gtcgctctag ctgtgggatt
     1741 ctttcgaaag caactgcaga ctgtgcagct cggtcatgtc cgccatgaat tacagtggtt
     1801 ggtagcgggg ctaatcatcc ttctaccgct tgtcttattt ctagctcgcc cgcatggccg
     1861 gttgcggcaa gtaggtcacg agattagcgt acaacttgga cactaccgac gccgaccgga
     1921 acgtcttttg ggcgccctcc tgacatctat gacgctgacg ctctgcaatg tgctgagttt
     1981 atggtactgc gcgcaagcgc tccatgcgca tctatctttt gtgacgatat tgctcatctt
     2041 cagcctggga gttggtgtcg gcaccgtcac gccgtcaccc ggcggcctgg gcggtgtcga
     2101 agctggttta gtggctggct ttgtggctta tcatctgacc ggcgcgcaag cgctggctgt
     2161 ggcactctta tatcggttga tcacctactg gctgccttta atagtcggcg ccttcgtatt
     2221 cgtcttgtgt gtgcgccgag agttatttag caggcagcag ctgactcatt agctcaatat
     2281 gttgacggct cgagctgcca ccagggcgat tgccaccaac gaaatcgtag cttgcaccag
     2341 catcatgagc ttagcccgac gagtcaacgg catggtatcg gtcgggctaa aggccatagc
     2401 gtttgtcgaa gagacataca tataatcgat aaaggttgca cgccatttgg ggtgtttata
     2461 ggattcgttt tgattctgtg gaaagagaaa gtcttgctcg tgtctggcgg cttgagctcg
     2521 acgacctggg ccacccccgt ccatttccca ataccagagg gcaaaaatga caatgttggt
     2581 caggtaaatg ttaacggcgg tcaataccaa gtcccggcca ttatgaacac gcccgctctg
     2641 aagtaacttg cgggctactt cgatcaaaga atagccatta gcgactccgg tcaggaaaat
     2701 cagcgcaaag acattgatgc gtctggacag cgaacgaaag atgcgctctt tcggcgtagt
     2761 gaaagatagg gcgattagta agacggcttc cacaaccaac aacaggtagc gagacccaag
     2821 cacataccga tccggcaaaa gcaactgcaa aataatagcc accagcatgg ttgtttgcac
     2881 atgccacacc ggatcgtgtt ccctgtattt attgccgagg atagccattg taggctattg
     2941 tactgcacgc cggctatcta tgtacagctg ctacatctgg atgcggttga agcgatttac
     3001 cgcaaaagtt aggcacaata caaatgtggc agccatgaca ccgatgtcaa cgctaaaatt
     3061 aaagtgtgcc tgattggata gcaaatggcg caaggcatcc accatgtagc tgaccggatt
     3121 gatggtactg attattttta accctatagg tatctgattg agcgggtaca gcgcactgga
     3181 taaaaagaac atcggtaaaa ttaaaagatt gttgataccc tggaagcctt gaaaatcatc
     3241 aagcatcgag gctatgccgg caccaaaaca agctagcgcc aggcacatta acaccagcac
     3301 caaaaaagcc ggtggaacca gaaaccagtt gtgcggccga aagccaaaga ttaatgagag
     3361 aatgaaaacc agcgtaccct gaataatcga aactgtcgcc ccacctagag cacttccgag
     3421 caaaattttg gagcgcttaa ccggcgaaac caaaagctcg gccaagaagc cgaagcgctt
     3481 atccatgagt attatcattc cccaaaaaac tccggacaat aagacagttt gagtgataat
     3541 ccccggcact agaaagttta aataattacc ttcgccggcc cgacggtaaa tggccccaat
     3601 accatagcct aacgccagca gcagcaagac cggctgaccc agcgaaccga gcaagcgtga
     3661 gccgctgcgc cagtagcgcc gaacttgcct gagccaaagc tgatacataa tctccattaa
     3721 cgcaacctca tatttcttcg agccatctga gcatttaatc cattgttgtt gcctgattca
     3781 tcccggacag ttgtgccggt cagttttaaa taagcttgct ctagggtttt tgttgcagtt
     3841 tgtttggtga gctcagcagt ggtaccacga gccacaattt tgccgtggtc gatgatgg
//
LOCUS       DATNGP010000094         2505 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_1552832, whole genome shotgun sequence.
ACCESSION   DATNGP010000094 DATNGP010000000
VERSION     DATNGP010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2505
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1552832"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..544)
                     /locus_tag="VN778_02310"
     CDS             complement(<1..544)
                     /locus_tag="VN778_02310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49835.1"
                     /translation="MVQLNLLPDVKLDYIRAQRSRRLVLSISVLVTGIVVLALVLLLL
                     ADVAQKKHLSDLSRDIQNESSELQQKPDISKILTVQNQLESLTTLHSGKPAASRLFNN
                     YLDQVTPAAASIASFDIDFTQQTVTITGTADALSTINKYVDTLKFTTYTTDSNKTPTK
                     AFSDVVLSSFGFANNAQDKSQ"
     gene            complement(550..1590)
                     /gene="pilM"
                     /locus_tag="VN778_02315"
     CDS             complement(550..1590)
                     /gene="pilM"
                     /locus_tag="VN778_02315"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HXR49836.1"
                     /translation="MSVLLSGTSDFFGLDIGTTAVRLVHLKGGGGTKALVKYAYVPIE
                     GNVSASDSKGDQQKLAQIIAQLVKQAGVGTRNVAVGIPSNRVFTTVADVDRLSSGELA
                     KAIPYQADSLIPTPVAESKLDWSVIGDSPADKTKQEILLTSVTNKFVEDRLDMLESVG
                     LNVVAFEPDNLAIARALASPEATPQMIVDVGHRTTDLVIVMNGAPHLTRSIPTGVEAI
                     IKAATQNLNVDQKQAEQFVFKFGLSKDKLEGQVFQAIGGTVDLLTTEIEKSIKFFGTR
                     YNGTKIERLIVTGGASVVPEFPLYLANKFGVNVEIGNAWRNVSFAQDRQNELLAISNQ
                     FGVVVGLAERTE"
     gene            complement(1758..>2505)
                     /locus_tag="VN778_02320"
     CDS             complement(1758..>2505)
                     /locus_tag="VN778_02320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49837.1"
                     /translation="PAYQDTPHMGGNYNSNFCLGEQKMPTSGATALGSIVSAGSLTGS
                     AKYTAPANTRIDGGTIPANTNAAIYVNGDVYISGANGGISYSNAGWNWSTTNDTVPSF
                     AVVAVGGNIYIDRNVTNLDGLYVAQKAGGNGGKIYDCTNGIGTANVVGVGSMFSDCNN
                     QLVVHGNLVADQINLMRTLGSLRDSLKNPGETPRGPIGRTCSDGGVYKVCAGEVFDFS
                     PELYLGKLQLTQSNNGATVYQAYTSLPPIL"
BASE COUNT          566 a          637 c          649 g          653 t
ORIGIN      
        1 cttggctttt atcctgcgca ttgttggcaa aaccaaaact gctgaggacg acatcgctaa
       61 acgccttggt cggggtcttg ttactgtcag tggtgtaggt agtgaatttc agcgtgtcga
      121 cgtatttgtt gatggtgctg agggcatcag ccgtaccagt tatggtgact gtttgctgtg
      181 taaagtcgat gtcaaaactg gcaattgagg ccgctgccgg tgtaacttga tcgagatagt
      241 tattgaatag ccgtgaggcc gccggcttac cgctatgcaa agttgttaag ctctctagct
      301 ggttttggac agtcaggatc ttggatatat cgggcttttg ctgcagctca ctactctcgt
      361 tttgaatgtc acggcttaaa tcgctgagat gcttcttctg agccacatca gcgagcagca
      421 ataataccaa agccagcacg acaatgccgg ttactaaaac agagatactg agcactaggc
      481 gacgagagcg ctgggcgcga atatagtcga gcttgacgtc cggcagaaga ttgagctgta
      541 ccatatcgtc tactccgtcc tctccgctag gccgacgacg acgccgaact gattagatat
      601 cgcgagcagc tcattttgcc ggtcttgggc aaagcttacg ttacgccagg cgttgccaat
      661 ctccacgttg acgccaaatt tattggccag atacagagga aattcaggca ccacgctggc
      721 accacccgtg acaatgaggc gctcgatttt ggtgccattg tagcgggtgc caaagaattt
      781 gatggatttt tcgatctcgg tggtcaacaa atcaaccgtg ccaccgatag cctggaagac
      841 ctggccttcc agcttgtctt tgctcaggcc aaatttgaag acgaattgct cggcctgctt
      901 ctggtccacg ttgaggtttt gggttgcggc tttgataatc gcttctacac cggttggtat
      961 actccgggtc aaatgcggcg cgccgttcat gacaatcacc aggtcggtcg tgcggtggcc
     1021 aacatcgacg atcatttgcg gcgtggcttc cggagaagcc agggcgcgag caatcgctaa
     1081 gttgtcaggc tcaaaagcaa cgacattcag ccccaccgat tccagcatgt cgagccggtc
     1141 ttcgacgaat ttgttcgtca cgctggtcag caggatctct tgcttggtct tatcggctgg
     1201 tgaatcgccg attactgacc aatctagttt agattcagcg actggtgtgg gaatcaatga
     1261 atcggcctgg tatgggatgg ctttcgccag ctcgccactt gataggcgat cgacatcggc
     1321 cacagtagta aagacgcggt ttgaaggaat gccgacagca acgttgcgcg tgccgacacc
     1381 agcttgtttg acgagttggg caatgatttg ggctaacttc tgctgatcgc ctttggagtc
     1441 actggccgat acattcccct caatcggtac gtaggcgtac ttaaccagtg ctttggtgcc
     1501 gccaccacct ttgagatgta ctaagcggac cgcggttgta ccgatgtcca agccaaagaa
     1561 gtcagatgta ccactgagta aaacgctcat aatctagccc cgattatacc ataagaggaa
     1621 tggtcggata atgtaccgtg gcgttcccgg tgtagagttg cgcgtagcgg taagctatgg
     1681 ttattaaacg ttttgtattt cccacccatt tttctgtttc ctactaaatg tgcttatcct
     1741 ttttatctta ccatacctta tagtatcggc ggtaaactcg tgtatgcctg atagactgtc
     1801 gctccgttat tcgattgggt cagctgcagt ttacctaggt acagctccgg actaaaatcg
     1861 aagacctcgc cggcacaaac cttatagaca ccgccgtcgc tgcatgtcct gccaatggga
     1921 ccacgaggag tttcacccgg gttctttagg ctgtcacgta atgatcccag ggtgcgcatc
     1981 aggtttattt ggtcagctac taagttgccg tgcacgacca actgattatt acagtccgag
     2041 aacatactac ctacgcctac gacgttggca gtgccgatgc cattggtgca gtcgtagatt
     2101 ttgccaccgt taccaccggc cttttgggct acatataagc cgtccagatt tgtgacattc
     2161 cggtcgatgt agatattgcc gccgacggcc accactgcaa aggatggcac cgtatcgttc
     2221 gtcgtgctcc aattccaacc ggcgttacta taggaaatgc cgccgtttgc accagaaata
     2281 tatacatccc cattcacata aatcgcagca ttcgtattgg ccgggatagt accgccgtcg
     2341 attctggtat tcgctggtgc ggtatacttg gcagatccag tcagactacc cgcactcacg
     2401 attgaaccca aggcagtagc acccgaggtg ggcatctttt gctcaccgag acagaagttg
     2461 ctattgtagt tccctcccat atgcggcgta tcctgatagg cggga
//
LOCUS       DATNGP010000095         2069 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_3574402, whole genome shotgun sequence.
ACCESSION   DATNGP010000095 DATNGP010000000
VERSION     DATNGP010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3574402"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(55..717)
                     /locus_tag="VN778_02325"
     CDS             complement(55..717)
                     /locus_tag="VN778_02325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49838.1"
                     /translation="MPQITIESAVLDRLPNLHVNEETLGAIEGHMQMAPNENVDMGIV
                     IAESARRRWPITGVGRYMSFGHPIGGYYGRHNEIRVYRKGASTPDEMTHSIIRGLAAR
                     YTQRGTDYEQRCAAQRLEFTTLFGGIAVGSALGNVPNVVEDVFLNHSPSLNQFLGDGT
                     VLGLIFGSVLGMAAGLHLTSKRYPNAGAGSLGYYIAEGITENAMKYYNEQLAPTAPLV
                     SQ"
     gene            complement(794..1579)
                     /locus_tag="VN778_02330"
     CDS             complement(794..1579)
                     /locus_tag="VN778_02330"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HXR49839.1"
                     /translation="MAYTRERSLPYKPGQVTPYKVSRSKIELFMQCPRCFWLDVRLKI
                     SRPSSPPFNINKAIDELFKKEFDVHRAAGTPHPIMVQNKLQAIPYAHNDLDTWRYNFT
                     GVTALHKPTNLHVFGAIDDVWVNDDGQLIVVDYKATSKNKEVSIDSDWQISYKRQLEV
                     YQWLLRQNGFEVSDTGYFVYTNARIDLDGFGDKLEFRTKLVPYTGSDTWIEPTLEKMK
                     ACMESDTMPPVGKAAMGGPCEFCSYARQRTELTLKHVNTRPPN"
     gene            complement(1617..>2069)
                     /locus_tag="VN778_02335"
     CDS             complement(1617..>2069)
                     /locus_tag="VN778_02335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49840.1"
                     /translation="QQVLQFLTPDFMSELLQVPEHTDIEIEGNKLFIISEVNDGNLYY
                     GFNEPTLQHLFATSNIALKHLNEVAFSWQASSSADTVHQMAATALVPRTKVMLGRRHV
                     GIGGLAGFIVYLLLNAAPSLGSKGLLVVLPVVCAIAVISLVVLWEQHH"
BASE COUNT          503 a          526 c          510 g          530 t
ORIGIN      
        1 aaaatttaag aaagaaaatc gcttgcgtgt atttatttta taactaatat aacgctactg
       61 gctaacaagc ggcgccgttg gagcaagttg ttcgttatag tacttcatgg cattttctgt
      121 gatgccttct gcgatgtagt accccaagct tcctgccccg gcattgggat agcgctttga
      181 agtaaggtgc aagcctgcag ccatgcctag cacacttcca aaaattaagc ccagcactgt
      241 cccatcgcca aggaactgat taaggctggg gctatgattt aagaatacgt cttccaccac
      301 gttaggtaca ttaccaaggg cagaaccgac agcgatgccc ccaaaaagag tagtaaactc
      361 cagcctttgg gctgcgcacc tttgttcata atctgtccct ctctgagtat acctcgcggc
      421 taagcctctg ataatcgaat gagtcatttc atcgggggta gaggcccctt ttctgtaaac
      481 tcttatttcg ttgtgccgcc cataataacc accaatagga tggccgaaag acatatacct
      541 gcctacgccc gtgattggcc accggcgacg agcactttcc gcaataacaa ttcccatgtc
      601 cacattttcg ttcggagcca tttgcatatg gccctcaata gctccaagcg tttcttcgtt
      661 aacatgaaga ttaggcaagc ggtccagaac tgctgattca atagtaattt gcggcatacc
      721 aatccctcac tactttatta tgtgaataaa aatagcacat cactttccca taaccatgag
      781 catgaagcaa aatttaattt ggaggtcggg tattaacatg tttaagcgtt agctcggtgc
      841 gctggcgggc gtagctgcag aattcgcaag ggccgcccat ggcggctttg ccgactgggg
      901 gcatggtatc ggattccatg caggctttca ttttttcgag tgtcggctcg atccaggtgt
      961 cgctgccggt atacgggacc agcttggtgc ggaactccag tttgtcgccg aaaccgtcca
     1021 gatcgattcg cgcattggtg tagacgaagt agccggtgtc actcacctca aagccattct
     1081 ggcgcagcag ccactgatag acttccagct gccgcttgta acttatctgc cagtcggagt
     1141 cgatgctgac ttccttgttc ttagaggtag ctttgtagtc gacgacgatc agctggccat
     1201 cgtcattgac ccagacgtca tcgatagcgc cgaatacatg gagattggtc ggcttatgca
     1261 gcgccgtgac accagtgaaa ttgtatcgcc aagtgtcgag gtcgttgtgc gcgtatggaa
     1321 tggcctgcaa tttattctgc accataatag ggtgcggtgt accggcggca cgatgaacgt
     1381 caaactcttt tttgaacaat tcatcgatgg ccttgttaat attgaatggc gggctcgatg
     1441 gtcgcgagat ctttagtcgc acgtcgagcc aaaagcagcg cgggcactgc atgaacagct
     1501 cgattttgga gcgcgaaact ttatacggcg tcacctgccc cggtttgtag ggcagcgagc
     1561 gttctctagt ataagccatt acatctattg tatcagccga ggagctttga ggcgccttag
     1621 tgatgctgct cccagagcac aaccagactt atgacggcta tcgcgcaaac tacaggcaac
     1681 actactagta ggcctttgct cccgagcgac ggagctgcat ttagcagcaa ataaacaata
     1741 aagcccgcga ggccgcctat gcccacgtgc cgtctgccga gcattacctt ggttctcggt
     1801 actagggcgg tagccgccat ttggtgaacc gtatccgccg aactggaggc ctgccagctg
     1861 aacgccacct cattcagatg ctttaaggcg atattcgatg tcgcaaataa gtgctgtaac
     1921 gtgggttcgt taaagccgta gtaaagatta ccgtcattga cctcactaat aatgaacagc
     1981 ttgttgccct cgatctcaat atcggtgtgt tcgggcactt gtaagagctc tgacatgaag
     2041 tctggcgtca aaaactgcaa aacctgctg
//
LOCUS       DATNGP010000096         5214 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_886565, whole genome shotgun sequence.
ACCESSION   DATNGP010000096 DATNGP010000000
VERSION     DATNGP010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5214
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_886565"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="VN778_02340"
     CDS             <1..228
                     /locus_tag="VN778_02340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49841.1"
                     /translation="KSFLDSQEAKDIRRALQQMALDSSYSTASSYSADSLTYPDNLIP
                     FIDKHMNYLNAHPKLDASMYLANIKLMSRVR"
     gene            401..913
                     /locus_tag="VN778_02345"
     CDS             401..913
                     /locus_tag="VN778_02345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49842.1"
                     /translation="MTPNPSNERLSQEEPPIIKVISDFYSAAYSAQMSGDNPIGTTMI
                     LGGRAEVIGLNRKRQANLGDNVRFLVIGGSLNFGGFSMDVGAQTLEMKYWIMKLTLSP
                     DDKRQIHELDPAQLNPVAVLFVDEDGKVSDGKDWIDEQETRTLLASLIDCELAPVLPA
                     WAQERETEND"
     gene            906..1412
                     /locus_tag="VN778_02350"
     CDS             906..1412
                     /locus_tag="VN778_02350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49843.1"
                     /translation="MTSPDKLMTRPDCEVACPLVTFCNNEIEKRREEIAAIGMSTLGA
                     AERYESTLQRYIEGRERLQELGGTFGEYFERPEAAQAAAALEAHLPKLRENAAGWIDT
                     KHRRLESLEAFKQNAKSTCHGPLSRKKYIILGEKVTRCSARYNKLHFLTFRAISHLPR
                     DFPADFHD"
     gene            1540..2136
                     /locus_tag="VN778_02355"
     CDS             1540..2136
                     /locus_tag="VN778_02355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49844.1"
                     /translation="MSEIDMKSIVGGLGVDEETVQRWKDAGLIKPKPLKGVLRRGNKE
                     DIQRAAEAGTLYGPMPEGLLTRLAKSVETPNEPNLPAGMVEKLSPGQKILFSFQAKSA
                     NLKTQETTDDLGNPQLRVFYKHIPVFRLAPPGTRLPIEGAGADTSGWRVEETRFPARE
                     QNLGSFVVDLASITDVLPGDVATWATQASVERCTDSRD"
     gene            complement(2084..3361)
                     /locus_tag="VN778_02360"
     CDS             complement(2084..3361)
                     /locus_tag="VN778_02360"
                     /inference="COORDINATES: protein motif:HMM:NF015957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HXR49845.1"
                     /translation="MNEVTKIHLGRQAFTISVDAHHELKNYLHAIEKQVDDKEVVEEV
                     ELRMAELLAEHGINASKVILPADVSFLKEQLGNPADFKDEDETTAPEAKPTDSKRLFR
                     DTDNAMLAGVAAGLATYFGIDALLVRLLFVLIVFVTAGGGILLYILLWLLVPEAKTPS
                     DRLQMAGKPVTVDSLKEIVENADVKGAANRANAALSGPINTLFRFILRLIGLVFVLSG
                     LSILFGLVTGEAYFLSRGGTWMQGNIFPSGVRENLVLHIAAAVAALMAVFIILFGVAI
                     FRRKWPIRTWITGVLAGLIFIGMAAGGALAADVYPTVHARYNANLHSTTRTVQPFTAV
                     NVDNMDVNLNYQVSDKYYVALSYYDHPNLVPVKTIVQNGILILDTRQFNELRNCQTLC
                     LPDTYNLTITVYSPNAINLENQYNVQPMPALPM"
     gene            complement(3358..3714)
                     /locus_tag="VN778_02365"
     CDS             complement(3358..3714)
                     /locus_tag="VN778_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009347329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HXR49846.1"
                     /translation="MNDTSDNTMQAQSEIQMRKGMLVYCVLLLLKDGKVYTSEIIHAL
                     RQAELIVVEGTLYPLLNRLAKDKLLAYEWQESEQGPPRKYYWLTDDGKSLLEELKATY
                     HRLHTSINALEKGKVK"
     gene            3824..4588
                     /locus_tag="VN778_02370"
     CDS             3824..4588
                     /locus_tag="VN778_02370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49847.1"
                     /translation="MMEGIPQDPDEYAERRRNMIAAITVGIEGEDYAFVIEIASHQNL
                     LRPFEIVEYMDVKRRSGENPTEKRRWEEGLGHYLAAIEEEEFPEKEEEAINAVVHRLE
                     FERAIDTDEVADEPRVEDDDPRSHYEGDDEDIDWRNDHFRPVGVDMKTLGEVSKTPVG
                     KSVVRRAEHAISTGNAQYLKDAINVIQEYGSNDEAVWALEKALDKFAFVYAKKQLERA
                     ANTGNNDYLKKAIRTLDQYVSSEARVKAMLAELGLS"
     gene            4666..5130
                     /locus_tag="VN778_02375"
     CDS             4666..5130
                     /locus_tag="VN778_02375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49848.1"
                     /translation="MSIRERLSKPGARKALVGGILGGVILGGSFNLVAGGYDNVPVAH
                     CHNRRLVDFPEWLGDTLPQRDIKDLARWLHTSYRNIEAGLGGTALCDVRFTNDQIESA
                     SAHARVKDISPNCLVLDQEGAKTDGQVSTQLFCAIPDETLGQSFTPIPSIHD"
BASE COUNT         1416 a         1189 c         1325 g         1284 t
ORIGIN      
        1 aaaagctttt tagattcgca agaggctaaa gacattaggc gtgcacttca gcaaatggcc
       61 cttgatagtt cgtacagtac tgcttcaagc tacagtgccg atagccttac gtacccagac
      121 aacctcatac catttatcga caagcatatg aactacttga atgcccaccc aaaattagac
      181 gccagcatgt atctagctaa tataaagctt atgagccgcg taagatagca caagccacgc
      241 taacctagcc catcctaaga gccctttggc tctgcacgct ccaactcaat tgcccgtatt
      301 agactatttc tatgtaacct gaaaattgtt taggacctct gacgaggttc tttttattgc
      361 tcatgcttgc aaagtatgta aaatctgtaa cacttctaaa atgacaccca atccaagcaa
      421 tgagcgactt tcccaggagg agcctccaat tataaaagtt atcagtgatt tctactcagc
      481 cgcctacagc gctcaaatga gtggagataa tcctatagga actaccatga ttctaggcgg
      541 aagagcggaa gtaataggat tgaatcgaaa aagacaggca aacttaggcg acaatgtacg
      601 ttttttggtg attggcggaa gtcttaactt tggtggtttt agtatggacg ttggcgctca
      661 aactttggag atgaagtact ggattatgaa gcttacccta tcccctgacg ataaaaggca
      721 gatccacgaa ttagaccctg ctcaactgaa cccagtcgct gtgctatttg tagacgaaga
      781 tggcaaagtg tccgacggca aagactggat tgacgaacaa gaaacacgga ctctactagc
      841 ttccttaatc gattgtgagt tggcgccagt acttcctgcg tgggctcaag aaagggagac
      901 ggagaatgac tagccccgat aagttgatga cgcgccctga ctgtgaggtc gcttgcccat
      961 tagttacgtt ttgtaataat gagattgaaa agcgccgaga ggaaattgca gctatcggca
     1021 tgtctacctt aggcgcagcg gaaaggtatg agagtacact gcagagatat atagagggca
     1081 gagagcgact ccaggaacta ggtggcactt ttggggaata ttttgagcgc ccagaggcag
     1141 ctcaagccgc tgcggcccta gaagcacact taccaaaact tcgggaaaac gcggccggtt
     1201 ggatcgatac aaaacatcgc cgccttgaat cactcgaagc ctttaagcag aacgccaaat
     1261 caacgtgcca tggccccctg tcccgtaaaa aatatattat tttaggtgaa aaagtgacca
     1321 ggtgttcggc tcggtataac aagctgcact tccttacttt cagggccata tcacacctcc
     1381 cccgtgactt tccggcagac tttcatgatt aacgccttaa gttcctagga tcaaaatgca
     1441 aagtgctgct ttatgctcat gcttgctaat gaaggtttta tggctttcgg tcgccttttt
     1501 atccttgcat atttagtaac taataggata atcatcacta tgagcgaaat tgatatgaag
     1561 agcatagtag ggggtctagg cgttgatgaa gaaacagtac aacgctggaa ggacgcaggc
     1621 cttataaagc cgaaacctct taaaggtgta ttaaggcgtg ggaataaaga agacatccag
     1681 cgggcagcgg aagcaggtac tttatacggg cctatgcccg aagggttatt aactcgatta
     1741 gctaaaagcg tggaaacgcc taacgaacca aacctaccgg ctggtatggt tgaaaaatta
     1801 agtcccggcc aaaagatatt attttccttt caggctaaaa gtgccaatct caaaacacag
     1861 gaaaccacag acgacctggg taacccccag ctgagagttt tttataaaca cattcctgta
     1921 tttagattag cgccgcccgg aacacgatta cccattgaag gggcgggtgc tgacacttct
     1981 ggctggagag tagaggagac tagatttcct gcacgagagc agaatctggg ctcttttgta
     2041 gttgaccttg cgagcatcac tgatgtttta ccaggggacg ttgctacatg ggcaacgcag
     2101 gcatcggttg aacgttgtac tgattctcga gattgatagc gtttggcgaa tacacggtta
     2161 tggtgaggtt atacgtatca gggaggcaca aagtttggca attgcgcagc tcgttgaatt
     2221 gccgggtgtc gagtattaag atgccgttct ggacgatagt ttttactggt accaagttcg
     2281 ggtggtcgta gtagcttaaa gctacgtagt atttatcgga aacttgatag ttaaggttaa
     2341 catccatatt gtcgacatta actgcggtga acggttggac ggtgcgggta gtcgaatgaa
     2401 ggttagcgtt gtagcgggcg tgaacggtag ggtagacatc ggccgccaaa gcgccgccag
     2461 ccgccatgcc gataaaaatg aggccggcca gcacgccggt tatccaggtg cgaatcggcc
     2521 atttgcggcg aaatattgcg acgccaaaca ggatgatgaa gactgccatg agggcagcga
     2581 ccgccgcagc gatatggagt accaggtttt cccggacgcc ggaagggaag atattgcctt
     2641 gcatccaggt gccgccgcgg gacaagaagt aagcctctcc cgtgactaag ccgaacaaaa
     2701 tacttaggcc cgagagaaca aataccagcc cgatcaatcg caagataaat cggaaaagcg
     2761 tattgatcgg gccgcttaag gcggcattcg cccggttcgc tgcacctttg acatcggcgt
     2821 tttcgacaat ctctttcagg ctatctaccg ttaccggctt gcccgccatc tgcaggcgat
     2881 cactcggtgt cttggcttcc ggtactaata gccatagcaa gatgtagagc aaaattcccc
     2941 cgccggccgt tacgaatacg atcaatacaa agagcagcct gaccagcaag gcatcaatac
     3001 cgaagtatgt agccagcccg gcagcgacac cggccagcat agcgttatcg gtatcacgga
     3061 acagtcgttt gctgtcggtt ggcttggcct ctggcgcggt ggtttcatcc tcatctttga
     3121 agtcggccgg gttgcctagc tgttctttga gaaagcttac gtctgctggc aaaatgactt
     3181 tgctagcgtt aattccgtgc tcggccagga gctcggccat tcggagctca acttcctcta
     3241 cgacctcttt gtcatcaact tgtttctcga tagcatgcaa ataatttttt aattcatggt
     3301 gggcatcgac cgagatcgtg aaggcctggc ggcccagatg gattttagtt acctcattca
     3361 tttaactttt cctttctcta gagcgttgat cgaagtgtgt aaccggtggt aggttgcttt
     3421 cagctcctct aacaagcttt ttccatcatc ggtcagccag taatacttgc gcggtgggcc
     3481 ttgctcactc tcttgccact cgtacgccag caatttgtct ttagccaagc gattcagcaa
     3541 agggtaaaga gtgccttcaa cgacaatgag ctccgcctgg cgaagggcgt gaatgatctc
     3601 cgaagtgtag actttgccgt ctttgagcag taaaagcacg caatacacca gcatgccttt
     3661 gcgcatctgg atttcgcttt gcgcttgcat agtattatca ctagtatcat tcatacacta
     3721 ctatgctata caaggtagta tattttgtca actacttaat tgctcatgct tgcgttgatt
     3781 catggattgc tggtattttg acagtaattg ttatggagca tagatgatgg aaggcattcc
     3841 ccaagacccc gatgaatacg ctgaaagacg gcgcaatatg attgccgcga ttacggtagg
     3901 catagaaggg gaggattatg cgttcgtgat tgaaattgcc tcccatcaaa acctgctgag
     3961 accttttgag atcgtggagt atatggacgt caaacggaga tcaggtgaaa accctaccga
     4021 aaagcgacgc tgggaagagg gtctcggcca ctacttggca gcgattgagg aagaggagtt
     4081 tcctgaaaaa gaagaggagg ctattaatgc cgtcgtccat cggctcgaat ttgaaagggc
     4141 aatagatact gacgaagtgg ccgacgagcc tagggtagag gatgatgatc cacgctctca
     4201 ctatgaagga gacgatgaag atattgattg gcgcaatgat cattttcggc cggtaggtgt
     4261 cgacatgaaa accttagggg aagtatctaa aacgccggta gggaaatcag tggtacgtcg
     4321 agccgaacat gcaatatcga caggcaatgc tcaatacctc aaagatgcca ttaatgttat
     4381 acaggagtat ggctcgaatg atgaagccgt ctgggctcta gagaaagcgt tagacaagtt
     4441 tgcgtttgta tatgccaaga agcagcttga gcgggctgct aacactggca ataatgatta
     4501 cctaaaaaaa gcgatccgca ccctcgacca gtacgtatcg tctgaggctc gtgtcaaagc
     4561 catgctagct gaactcggct tatcttagct aagcgagaga tatattgacc aactacttct
     4621 tgctcatgct tgcagataag cgagataagg ctatattagt agcttatgag cattagagag
     4681 agattaagca aacccggagc ccgcaaggct ctcgttggcg gaattttagg tggagtaatt
     4741 ctcggcggca gctttaacct ggtggcaggt ggctatgaca atgtccctgt cgcacactgt
     4801 cacaatcgaa gactagtcga ctttccagaa tggctcgggg acactttgcc tcagcgtgac
     4861 attaaggatt tggcaaggtg gttgcatacc agctacagga atatagaagc aggcttgggc
     4921 ggaacggcct tgtgtgatgt gagattcacg aacgatcaga tagaaagtgc ttctgcccat
     4981 gcgcgggtca aagatatcag ccctaactgc ctggttttag accaagaggg agcgaaaacg
     5041 gacggtcagg tgtcaacgca actattctgt gccataccgg atgaaactct tgggcaaagc
     5101 ttcacgccta ttccctcgat tcacgactaa gtctcaggtg gtcggaacta tatcacacgc
     5161 ggacaagcgt atatttaact gcgcgtccca tgcagagctc ctacagtaga gcct
//
LOCUS       DATNGP010000097         5566 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_2169904, whole genome shotgun sequence.
ACCESSION   DATNGP010000097 DATNGP010000000
VERSION     DATNGP010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5566
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2169904"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(232..1401)
                     /locus_tag="VN778_02380"
     CDS             complement(232..1401)
                     /locus_tag="VN778_02380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49849.1"
                     /translation="MNLLKKIATVMATVGFALAVTTASGAAAQPSCYQYNANGFPTSQ
                     TSVFNDICGAPGVGNESDFVRLRQSANGNDEDFQNNPQFNPGNITAACNDGDKFDVWN
                     YVHNDAESQFNDNGSGTAVAHNVQSLMKADLGKTGTSFNFSDTVSASNAAATVSDHVT
                     LNCNNGSQVTLNLVPGSVHLVSAAVANGAWQDMSDGTINSAVKLGSPTMGSGDQWGCW
                     NYRIVIVYQVTVKKVIPTPPQVTATCDMFTVTASEDRKVTINQFKFTAVNANFKNVVV
                     NWGDNHSDSFTNSVVGQTHQLPASPNQFEIMATANFVDVNGKSISSTNANCAQEVKFT
                     PNQPPTITTPPTSTTTPPTQLVNTGAGSVLGLFAAASAAGATLYRRALSRKLSRQ"
     gene            complement(1430..2944)
                     /locus_tag="VN778_02385"
     CDS             complement(1430..2944)
                     /locus_tag="VN778_02385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49850.1"
                     /translation="MSTEGHSTPVMPERQATPVMEEHQVVAQQRNGFMRRAMRRGLPL
                     VVVAAAGAGAAYVATGGENHNTATADASGNVPTLVSNPNQALSNCDNLEFDNAAADAS
                     SYNSDAFLPSTTVDTPEGAKQYVFNLFSDHGPLAGKGDMESFAAMMSTVYGASHQGGF
                     ENTNYDYIDQFNKALGRYQGVDGRDRARDDCKNLFNTLTQDVGYNDNWANSGETVTQF
                     AAVRDNGDNHITGATMFRGTVKEGVTLEGVEFKLRTTSKGENGYLSVLVSTNSKGVEN
                     GTIFIKGLTVQQFNGKSQTKQGKNPAKRTHELHPSNHGQQQIASGLGHIMVRRSSNGG
                     LVLSSGTSSSQEQQSGQSTGGSSTPSGRNHGGKNHSGTGSEGQSGGGGGGSNHGSTEG
                     SGGSASGSGGSGTGGAGSAGGGSGSGEGNGGSGAGTGGSGGGSGGGGETGTGTTTPTT
                     PTETTPTTPTHTTPTTPTETTPTTPTETTPTTPTTPTVKDPTTCTTVSPFCPQG"
     gene            complement(3135..3983)
                     /locus_tag="VN778_02390"
     CDS             complement(3135..3983)
                     /locus_tag="VN778_02390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49851.1"
                     /translation="MSLLPETQDALLEFGASQEYMFGLHEANEPRAGMQGRNIAEVPF
                     DPVRHSPLAINPFSVWEQVLGVPHVKVNIPGRPLNANVVIHQPASEGLDHTYRYGEHR
                     RWLARKLGTAIIDSMPGITDRTWVYSIGEAADEVTDPDTEVITTNGDPQEESARIAEI
                     CKSGLTFVVSGFLRLPLHEQDKATFPSTVGVKANVLTDRGIVPSGLVVRSGVKAGEVN
                     TANSDDVQENSELLEQQHTRVVKRLKGTGIAVASVVLARKVAYGFEAAVADAAIARAV
                     RQVTPK"
     gene            complement(4002..5006)
                     /locus_tag="VN778_02395"
     CDS             complement(4002..5006)
                     /locus_tag="VN778_02395"
                     /inference="COORDINATES: protein motif:HMM:NF019346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXR49852.1"
                     /translation="MAVVAFEQMASTNEALGYYEDGMSELGRALHGEEHAKQAILIGA
                     VILRPFILSGIPAGGKSTLMQNAWRLYGGVDYEDVARIPGQHDLTATQLVGGEIATYN
                     GRREKSLVVVEGLVKPSTRAIVVEEMNRTNPEAVNSLLPVLEDRRLETTAGHVAIPNL
                     VYVGVAMNPSESRQSTFPVSDAMASRLTLGAVLGVKGQRDERLANVRAIREHKKKPEN
                     GAENIRPIYDAAHLPGLRNYVMATRLSETMSAKIDEVTINTSDALESHGIHETDNRLP
                     LHIEMNAMALGSFRTEEHIVRDEDIVDAVYLVLAGRFGAKKQTNGLNLDALAREITQF
                     "
     gene            complement(5009..>5566)
                     /locus_tag="VN778_02400"
     CDS             complement(5009..>5566)
                     /locus_tag="VN778_02400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49853.1"
                     /translation="FDHTNAEAVVGNIGQVAVMPSSRFSAYTETTQGAPAGEAPLSQQ
                     VSLALNKVAESNTKNHKADGRIVIITNGNRVGSINDLAASATQQHTAIYTVNVERKGT
                     PRNLVGQLEKLSRETGGQFWSANSHNLSSVAKGVKETTSPDQARLPQPDRWPERISGL
                     LALFGAGIVYRNRKRLPTGKNSKGE"
BASE COUNT         1241 a         1448 c         1439 g         1438 t
ORIGIN      
        1 ctagacgatg ggacccaaaa agtccctgcg ggactttttg tagactcgag cgaagcgtcc
       61 tttcaagacc ggactatcat accataacag aggtaaaaag aaaatccctc gctcgcatcg
      121 agggattttc aattctttgt atgtagtcta gagcggtcag tcagtgctca tgtctcaaac
      181 taactgtacc ttagcacaaa atgcgagttt gcgctagtgg ttatccacag tttactgacg
      241 gctgagcttg cggctcaaag cgcggcggta cagcgtagca cctgctgcag acgcagcggc
      301 gaacaagccg agcacgctgc cagcaccggt gttgaccaac tgggttggag gtgtagttgt
      361 actggtcgga ggcgttgtga tagtcggcgg ctggttaggc gtaaacttca cttcttgggc
      421 acaattagca ttcgtagagc taatagactt accgttgacg tctacgaaat tcgccgtggc
      481 catgatctcg aactgattcg ggctggccgg caactgatgc gtctggccga cgacactgtt
      541 cgtaaagctg tcactgtggt tatcgcccca gttgactact acgtttttga agttagcgtt
      601 aacggctgtg aacttgaact gattgatagt tactttgcga tcttcgctag ccgtaactgt
      661 gaacatatca caggtagccg tcacttgtgg aggtgttggg atgaccttct taaccgttac
      721 ttggtaaacg atgacaatgc ggtagttcca gcaaccccac tgatctccac tgcccatggt
      781 tgggctgccc agcttaacgg cggaattaat cgtgccgtct gacatatcct gccaagcacc
      841 atttgctacg gctgcgctga cgaggtggac actgcccgga accaagttca gggtaacttg
      901 agaaccattg ttacaattca gggttacatg atcggaaacg gttgcggccg cgttagaagc
      961 agaaaccgta tcgctgaagt tgaagctcgt accagtcttg ccgaggtcgg ccttcatcaa
     1021 agattggacg ttgtgggcaa cagcagtacc cgaaccattg tcattgaact gagattcggc
     1081 atcgttgtgg acgtagttcc aaacgtcgaa cttgtcgcca tcgttacagg cagcagtaat
     1141 gttgccggga ttgaactgcg ggttattttg gaagtcttcg tcattgccat tagcactctg
     1201 acgaaggcgc acgaagtcag actcgttacc gacgcccggc gccccacaga tgtcgttgaa
     1261 gacggatgtc tgagaagtcg ggaagccatt cgcattatat tgatagcaag atggttgcgc
     1321 tgctgcgcca ctagccgtgg ttaccgcaag cgcgaagcct acagtggcca ttacggttgc
     1381 gattttcttt agtaagttca tatgattgct ctccttatac cctgcttatt taaccttggg
     1441 gacagaacgg actgaccgta gtacatgtag tggggtcttt aactgttggt gtggtgggcg
     1501 tggtaggggt agtctcggta ggtgtggttg gtgtggtctc ggtaggtgta gtaggtgtcg
     1561 tgtgagtggg cgttgtagga gtagtctcgg taggtgtggt tggggtagtg gttcccgtgc
     1621 cggtttcgcc accgccacct gagccgccac cgctgccacc ggtgcctgcg ccacttccgc
     1681 cattaccttc gccgctgcct gagccaccgc cagctgagcc agcgccacct gtgccgctgc
     1741 cgccactgcc tgaagcagat ccgccggaac cttcagtcga gccgtgattc gagccaccgc
     1801 caccaccgcc actctgacct tcagagccgg taccggaatg gtttttacca ccatgattgc
     1861 ggccgctagg tgtactagat ccgccagtgc tctggccact ttgttgctct tggctacttg
     1921 aagtgcctga gctcaagacg agaccgccgt tcgaactgcg gcgtaccatg atatgaccaa
     1981 ggccgctggc aatttgctgc tggccgtggt tagaaggatg cagctcgtgc gtgcgcttag
     2041 ccggattctt accttgcttg gtctggctct taccattgaa ctgttggaca gtcaggccct
     2101 taatgaagat tgtgccgttt tccacgcctt tggagttggt ggaaaccaag acgctcaagt
     2161 aaccattttc acctttagaa gtggtgcgaa gcttgaattc aacaccttcc agggtaacgc
     2221 cttctttgac agtgccgcgg aacatggtag ctccggtaat gtggttgtca ccattatccc
     2281 gaacggctgc aaactgcgtg actgtttcgc cggaattggc ccagttatcg ttgtacccaa
     2341 cgtcctgggt tagtgtgtta aagaggttct tacagtcatc acgggcacga tcacgtccgt
     2401 caacaccttg gtagcgcccg agggccttgt tgaattgatc aatgtaatcg tagttggtat
     2461 tttcaaaccc accttgatga gatgctccat agacggtaga catcatagcg gcgaatgatt
     2521 ccatatcgcc cttgccggcc agcgggccgt gatcactaaa gagattgaag acatactgct
     2581 tggcaccttc tggtgtgtca acagtggtgc ttggcaagaa agcatcggag ttatagctgg
     2641 aggcgtcagc tgctgcgtta tcaaactcga gattatcgca gttgctaagc gcttggtttg
     2701 gattactaac taaggtaggt acattgccac tagcgtccgc agtagccgta ttatggttct
     2761 cgccaccggt agctacatat gcagcgcctg ctccagccgc agcaacaacg acgagtggca
     2821 acccacggcg catcgcgcgt cgcatgaagc cgttcctttg ctgtgctacc acttgatgct
     2881 cttccatcac cggtgttgct tgcctctcgg gcataactgg tgtcgagtgt ccttctgttg
     2941 acatgtatta ctcctttaac tcctaattgc ctaacaataa aaaagcgaga attaataacc
     3001 accctcgctt ttccttacct tcttttgggg tgcttgggcc tatttgacca aagcatgttt
     3061 ttatgatctg agtatatcac tctctttgtt atttgtcaac tatataatat aaatatcttg
     3121 cttaagctta cgagctattt cggcgttact tgccgaaccg ccctagcaat agctgcatcc
     3181 gcgactgcgg cttcaaaccc atatgccact ttgcgggcca gaactactga tgcaacagcg
     3241 atgccagtcc cttttaaacg cttgacgacg cgtgtatgtt gctgctctag cagctcacta
     3301 ttttcctgaa catcatctga attagccgta ttcacctcgc cggcttttac accgctgcgt
     3361 accactaatc cactcgggac tatccctcta tcggtcagta cgtttgcttt aacacctacg
     3421 gtggatggaa aagtggcttt atcctgctcg tgcagcggca aacgtaggaa tccgctgacg
     3481 acaaaggtta acccactttt gcatatttcg gcgatgcggg cgctttcttc ctgaggatcg
     3541 ccattggtgg taattacctc agtatcagga tcggtcacct cgtcggctgc ttcaccgatg
     3601 gagtagaccc atgtgcgatc ggtaatgcca ggcatagaat caatgattgc cgttccgagc
     3661 ttgcgggcca gccaccggcg atgttcacca taacgatagg tatgatccaa cccttcagaa
     3721 gcaggttgat ggataacaac attggcgttc agcggccgcc cggggatatt aacctttacg
     3781 tgtggcacgc cgagcacttg ctcccagacc gaaaagggat taatagccaa ggggctgtga
     3841 cggacaggat caaatggaac ttcggcgatg ttgcggccct gcatacctgc tcgcggctca
     3901 ttagcctcgt gcagaccaaa catatactct tggctagcgc caaactctaa aagagcatct
     3961 tgagtttctg gtagcagcga catggtgtct gcctttcctt cttagaactg cgttatttca
     4021 cgagccagag cgtcaagatt gaggccgttt gtctgcttct tggcaccaaa ccgcccggcg
     4081 agaactaggt agacggcatc gacaatatct tcgtcacgca caatgtgctc ttcagtacgg
     4141 aaactgccta gcgccatcgc gttcatttca atatgcagtg gcaaacggtt gtcggtttcg
     4201 tgaatgccgt gactttccaa agcatcagaa gtattgatcg tgacttcatc aatcttggct
     4261 gacatagttt cacttaaccg ggtagccatg acgtagttgc gcagcccggg tagatgagca
     4321 gcgtcataga tcggcctaat attttctgcc ccgttctcag gtttcttctt atgctcacgg
     4381 atagctctca cattggccag gcgctcatca cgctggcctt tgacaccaag tacggcgcca
     4441 agcgtaaggc gtgaagccat ggcatcggag accgggaaag tggactgtcg ggactcggaa
     4501 gggttcatag caacaccgac ataaaccaga ttgggtatcg caacgtgtcc agccgtcgtt
     4561 tccaggcggc gatcctcgag tactggcaaa agtgagttga cagcttcagg gttagtgcgg
     4621 ttcatttctt cgactacgat agcgcgagta gagggcttaa ctaaaccttc aactacgacc
     4681 aggctttttt cgcggcgccc attgtaagta gcaatttcac caccaactaa ctgggtagcc
     4741 gtcaagtcat gttggccagg aatccgggct acatcttcat aatcaacgcc accatataat
     4801 cgccaagcgt tctgcatcag cgtcgattta ccaccggctg gaatacccga caaaatgaaa
     4861 ggacgtaata taactgcacc gatcaaaata gcctgcttgg catgctcttc accgtgcaat
     4921 gcccgcccta gctcgctcat accgtcctca taatatccga gggcctcgtt ggtacttgcc
     4981 atttgttcaa aagctactac tgccataatt attctccttt cgaattcttg cctgttggta
     5041 ggcgtttacg attcctataa acaattccgg caccgaatag cgccaaaaga ccactgatac
     5101 gttccggcca acggtcgggt tgtggcagac gggcctgatc aggtgaagtt gtctctttca
     5161 cgcccttagc gacactggaa agattgtgac tgttagcgct ccagaactgg ccgccggtct
     5221 cacgagacag cttttcgagc tggccaacta aattccgcgg cgtgcctttg cgctcaacgt
     5281 taactgtata aattgcggtg tgctgctggg tagcgctggc ggctaaatca ttgatgctgc
     5341 caacccggtt gccattcgtg ataatgacta ttcggccatc agctttgtga ttcttagtat
     5401 tgctctcggc aaccttattc aaagctaaac taacttgctg gctcagggga gcttcgccgg
     5461 ccggagcgcc ttgggtggtt tcagtgtagg cgctaaatcg actggagggc atgactgcca
     5521 cctggccgat attgccaaca acagcctcag cgttcgtgtg atcaaa
//
LOCUS       DATNGP010000098         6227 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_3606489, whole genome shotgun sequence.
ACCESSION   DATNGP010000098 DATNGP010000000
VERSION     DATNGP010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6227
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3606489"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..465)
                     /locus_tag="VN778_02405"
     CDS             complement(<1..465)
                     /locus_tag="VN778_02405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49854.1"
                     /translation="MAQPNKPSFPDYVLFAGEAAQYALHKSRLDRLRPVEAAIAQLVG
                     SDCEAYEQLLWLPPRKIKLVDVTTVVMTPSVPNSPRLAKNWTPTSVISVDGEAIEALE
                     VEDPQAVEAAAYRSEKAWHVGIQAVVGARIFEPLDGSRKAFSVRQGGGYAEFT"
     gene            complement(476..2440)
                     /gene="uvrB"
                     /locus_tag="VN778_02410"
     CDS             complement(476..2440)
                     /gene="uvrB"
                     /locus_tag="VN778_02410"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015893504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HXR49855.1"
                     /translation="MASFKLKADFGPMGDQPTAIAQLTKGLEDGLHHQTLLGVTGSGK
                     TFTMANVIQNVQRPTLVLSHNKTLAAQLYTEFKHFFPDNAVHYFVSYFDYYQPEAYIP
                     RSDTYIEKDSQINEEIDRLRHAATDALLSRKDVIIVASVSCIYGIGSVEDYSGLSIGV
                     QRGERKVRDKFLRQLTDIQYQRNDIDFHRSTFRVRGDVVDVFPAGEELAYRMEFFGDE
                     VERITKIDPLTGEILANLDSYKIFPGSHYVTPQEKLKIALGQIEQELAERLALFKKEG
                     KLLEAQRLEQRTRFDLEMLEETGFVKGIENYSRYLTNRSPGEQPATLLDYFPDDYVMM
                     IDESHMTIPQVRGMYNGDRARKEVLVEHGFRLPSALDNRPLTFTEFERHLNQAMYVSA
                     TPAEYELSRSPEPAQQVIRPTGLLDPPIEVRAIEGQIDDLIAEIRSTVDKHQRVLVTT
                     LTKRMSEDLAEYLNEINIKVAYLHSDIDTLERSDILRDLRLGVFDVLVGINLLREGLD
                     LPEVSLVAILDADKEGFLRSEQALIQTVGRAARHEEGHVIMYADHVTASMQRTIDETT
                     RRRKIQEAYNTEHGITPTGIQRAIDKGMRPELPEEAKRAKLDLKKIPKDEYGSLIKDL
                     TSQMDLAAANLQFEQAAELRDLIDEIKQKV"
     gene            complement(2462..3070)
                     /locus_tag="VN778_02415"
     CDS             complement(2462..3070)
                     /locus_tag="VN778_02415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49856.1"
                     /translation="MKKSLSQKGFTLVEGLLIIIALVLVVFVGYYVWHNQKSTNKTVN
                     MTSQNAHKTEQSKTSASQNLFKLTNVGVQFELPSSLSDFQYTPQTYSDGSVGGYISTA
                     AIENTIKQCIAGQGDQAQQADAESLAFAGLGKLEGQYDPNQVEEGQLLKQFSSFYLTI
                     SYPNGSSPCYSDQNAMDQLQSQEHAASDAFTKVFQSSATEIK"
     gene            complement(3089..3691)
                     /locus_tag="VN778_02420"
     CDS             complement(3089..3691)
                     /locus_tag="VN778_02420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49857.1"
                     /translation="MKLHQKGFAALELVLILVIVGLIAGVGYFVLQSNQKANTTLSSA
                     SSATQTKVAKSDSVISADKSQLPNGWKAVVDTPATISLADTNVKCAVLVSQTASPSLN
                     AAGAQAFVGRKLAALDQGNASSSKTTVGTAADQAVGGQTVSMYPVTVKDTSKDSFTDY
                     GKAGYVVGKGYYVSINETCQNQQANLSQADAALQAVTIKS"
     gene            complement(3759..4412)
                     /locus_tag="VN778_02425"
     CDS             complement(3759..4412)
                     /locus_tag="VN778_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008578597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49858.1"
                     /translation="MTPTKPDGTKMLRYSICVSGAASGDTVQASAGLAREAGAAIARS
                     GHILTTGATVGLPYYAALGAKEAGGMSVGFSPAVSLREHLRKYRLPSNCFDFINFTGL
                     HYVGRDLYLVQSSDAVITVGGRFGSLHEFTSALEAHKPCGILTGSGGTADQIPKLMEA
                     LEPPNGDLVIYDEDPERLVSRIVAILDEKYADIRNQLENHDEHWFLDLDTPDRPHAG"
     gene            complement(4409..5653)
                     /locus_tag="VN778_02430"
     CDS             complement(4409..5653)
                     /locus_tag="VN778_02430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49859.1"
                     /translation="MTRFLSEILQAPEPFFRLGLRKLEAANGHPSTDIRFSTEVMHAS
                     QAKLRQLGLDPHDTTAEELYHALQERVQADDARLTKKLRTLAATHISAEGEVVAGMVH
                     ALQELPDTKRCFALKGSAVKTLIKKLPPKKAMRELGYRSLDSFVKHESPITMLAAAWL
                     SENSSWQHRWLDQYKRLKPSDFENRSIALVQPNTKRWRELADSVVARERHNLLSFKEL
                     GAVVFLPLPATVPNGTTLVSTSLALHELNEIRAASTFLKLCQVRPDFGKIVQTVVSEE
                     PNVKADLLDQPVPWRLIQRHYARLTHEALSQAFEPHVQLEDMIWHPLEQSLSAIESSL
                     SFWHGSEHVGLLQGGRPVSFNLLDAALNYCNKLPFEQRLTHYFQQSLWHELLLRYLKH
                     DAVEQAVTLQLQPQLAEEALVI"
     gene            complement(5747..>6227)
                     /locus_tag="VN778_02435"
     CDS             complement(5747..>6227)
                     /locus_tag="VN778_02435"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HXR49860.1"
                     /translation="AFQPGTFQMEAGAERLQRIYARSEVLILNREEAVLVGGGNHEDI
                     HDLFDHLHALGPRVVVITDGPNGAYASDGQSRFQMPLYPDPAPPVDRTGAGDSFASTF
                     VAALMKGNTIEGALQWAPINSMSVVQKVGAQAGLLTEKELEHFLRQAPDWYKPTPLK"
BASE COUNT         1438 a         1610 c         1590 g         1589 t
ORIGIN      
        1 ggtaaattca gcgtagcccc ctccttgacg gacggaaaaa gccttgcgcg acccatcgag
       61 aggctcaaag atgcgtgcac cgaccaccgc ttgaatgccg acatgccagg ccttttccga
      121 gcggtaggcg gcagcttcta cagcctgagg gtcctccact tctagcgcct ctattgcctc
      181 accgtcaaca cttataacgc tggtgggtgt ccagttttta gctaagcgcg gcgagttagg
      241 gaccgaggga gtcatgacca ccgtggtcac atcgaccagc ttaattttcc taggcggcag
      301 ccacaacagc tgttcgtatg cttcgcagtc cgaccctacc aattgcgcga tggcagcctc
      361 aacgggccga aggcggtcaa ggcgagactt gtgcaaagcg tactgggccg cctcaccggc
      421 aaacaggaca taatcaggga aggagggctt gttgggctgt gccatggcct aaaaactaca
      481 ctttttgctt gatttcgtca atgagatccc ttaactcggc ggcttgttca aactgcagat
      541 tggcggcggc cagatccatt tgagaagtca gatctttgat caatgagcca tactcgtctt
      601 taggaatctt cttaaggtca agcttggccc gtttggcttc ctcaggcaac tcgggacgca
      661 tacctttgtc gatcgccctt tgaatacccg tgggcgtaat gccatgttcc gtgttatagg
      721 cttcctggat cttacggcga cgcgtggttt catcaatggt ccgctgcata ctggccgtaa
      781 catgatcggc gtacatgata acgtggcctt cttcgtgacg ggcggcacgg ccaacagttt
      841 gtatcaaagc ctgctcgctt ctcaaaaagc cttctttgtc ggcatccaaa atggctacca
      901 gcgacacctc cggcaagtcc aaaccttcac gcaacaagtt aatacccact aatacatcga
      961 agactccaag ccgtaaatcg cgcaaaatgt cgcttcgttc cagcgtatca atgtcgctgt
     1021 gcagataagc aactttgata ttgatttcat ttagatactc cgccaaatct tcagacatgc
     1081 gtttggttag ggtcgtcacc aatactcgtt gatgtttatc gacggttgat cggatttcgg
     1141 caatcagatc atcgatctga ccctcgatcg cccgcacttc aattggcgga tccaataatc
     1201 cggtcggtcg gataacttgc tgggccggct cagggctgcg actcaactca tactcggcag
     1261 gggtggcgga tacgtacatg gcttgattga ggtgccgttc gaattccgta aaggtcaggg
     1321 ggcgattatc caaggcgctt ggcaggcgga agccgtgttc taccaagact tctttgcgtg
     1381 cccggtcacc attgtacata ccgcgtactt gcggaatggt catgtgcgat tcgtcgatca
     1441 tcatgacata gtcatcgggg aaataatcga gcaaggtggc cggctgctca cctggcgacc
     1501 tattggtcag atagcgggag taattctcta tgcccttaac aaagccggtt tcttccagca
     1561 tttccagatc aaaacgtgtc cgctgttcca gtcgctgagc ctccagcaat ttgccctctt
     1621 ttttaaaaag cgctaagcgt tccgccagct cttgctcgat ttgtccaagt gcaattttca
     1681 acttttcctg cggtgttacg tagtgtgatc ccggaaagat tttatacgaa tcgagattag
     1741 ccaggatctc accggtcagg gggtctatct tggtgattcg ctcaacttca tcaccaaaaa
     1801 actccatcct ataagctagc tcttcgccgg ccgggaaaac atcgacgaca tcgccgcgaa
     1861 cccgaaaagt actgcggtga aagtcaatgt cattacgctg gtactggatg tccgtcagct
     1921 gcctcaggaa tttgtcccgg actttgcgct cgccgcgttg cacaccgata gataggccgc
     1981 tatagtcttc caccgagcca ataccataaa tacagctgac actggccacg ataatgacat
     2041 ctttgcggct aagcagtgca tcagtagccg cgtgtcgcag gcgatcgatc tcctcattga
     2101 tctgcgaatc tttctcgata taggtgtcgg agcgcggaat gtacgcttcg ggctgataat
     2161 aatcaaaata gctcacaaaa tagtgcacgg cgttatcggg aaaaaagtgc ttgaactctg
     2221 tatacagctg ggctgccaac gtcttgttat gactcagtac taacgtcggc cgctgcacat
     2281 tctgaataac gttggccatc gtaaatgtct tgcccgaccc cgtcaccccg agcaaagtct
     2341 gatgatgcag cccgtcctct agccccttgg ttaattgagc aatcgctgta ggttgatcac
     2401 ccatcggccc aaaatccgcc ttcaacttaa agctagccat ccttatattg taacaagcga
     2461 tctatttaat ttcagttgcc gatgactgaa agaccttggt aaaggcgtcg gatgcggcgt
     2521 gttcctggct ttgcagctga tccatggcat tttgatcaga ataacaaggg ctgctaccgt
     2581 tcgggtatga gatagttaga tagaaggagc tgaattgttt gagtagctgt ccctcttcca
     2641 cttggtttgg atcatactgg ccttccagct taccaagtcc agcaaaagcg agcgattccg
     2701 catcggcttg ctgggcttga tccccttggc cggcgataca ttgctttatg gtattttcta
     2761 tagcggccgt tgatatatag ccgccgacac ttccgtcact gtaagtttgc ggcgtatatt
     2821 gaaagtcact tagtgaactt ggtaattcaa actgaacacc gacattagta agcttaaata
     2881 ggttttggct agccgacgtc ttgctttgtt ccgttttatg ggcgttttgg ctagtcatgt
     2941 taactgtttt gttggtgctt ttttgattat gccagacata gtagccaaca aaaaccacaa
     3001 ggacgagggc gattattatc aacaagccct cgacaagcgt aaagcctttt tgactcaatg
     3061 attttttcat gtgcgtggcg gcagatattt aagacttaat ggtgactgct tggagtgcgg
     3121 cgtcggcttg tgacaggttt gcctgctggt tttggcaggt ttcattgatt gaaacatagt
     3181 aacctttacc tacgacatag ccagcctttc catagtcagt aaaggaatct ttgctagtat
     3241 ccttcacggt caccgggtac atgctcacgg tctggccacc cacggcctgg tcggcagccg
     3301 ttccaacggt agttttcgag ctactagcgt taccctggtc aagtgccgcc aatttcctgc
     3361 ctacaaaagc ctgggctccc gcagcgttca gtgacgggct ggccgtctgg gagaccagaa
     3421 ctgcacattt cacattggtg tcggccaggc taatggtagc tggcgtgtca accaccgctt
     3481 tccagccatt cggcagctgg gacttgtcag cggaaattac cgagtcggac ttggccactt
     3541 tagtctgcgt ggcacttgag gcgctggaca gcgtcgtatt agccttttga ttgctttgga
     3601 gtacaaaata accaacaccg gctattaagc caacaataac gagaatgagg actaactcta
     3661 gggcagcgaa acctttttga tgtaatttca tatgattaaa tatcctactt atgcttttgc
     3721 aataattgtg cgctagccta agcaggttgt caatctattt aaccggcgtg gggccgatca
     3781 ggtgtgtcga ggtcgagaaa ccagtgttcg tcgtgatttt cgagttgatt acggatatcg
     3841 gcgtattttt catcgaggat ggccacgatg cgacttacca ggcgctcagg gtcttcgtca
     3901 tagattacta ggtcgccgtt ggggggctcg agggcctcca tgagcttggg gatttggtcg
     3961 gccgtgccgc cgctgccagt aagaatgccg cagggtttgt gagcttccag agccgaggtg
     4021 aattcatgga ggctgccgaa acggccacca acagtgataa ccgcatcact ggactgaact
     4081 aagtagaggt cgcggccaac atagtgaagt ccggtaaagt tgatgaaatc gaagcagttg
     4141 ctaggcagcc ggtatttgcg caaatgctcg cgcaagctga cagctggcga gaagccaacg
     4201 ctcataccgc ctgcttcctt agctcccagc gccgcataat atggcaatcc gacagtggcc
     4261 ccagtagtta aaatatgccc gctccgagcg atagctgccc cagcttcacg agccagacca
     4321 gccgaagctt gaaccgtgtc gccagatgcg gcgcctgaca cacaaatgga atagcggagc
     4381 attttggtac catcaggttt ggtgggtgtc atatgaccaa agcctcttca gctagttgcg
     4441 gctgcaattg caaggtcacc gcttgctcaa ctgcatcatg tttcaaatag cggagcaaca
     4501 actcatgcca tagtgactgc tggaaatagt gcgtcagtcg ctgttcaaac ggcagcttat
     4561 tgcaatagtt caaagcagcg tcgagaagat taaaagacac cggtcggcct ccctgcagca
     4621 agccaacatg ttcgctgccg tgccagaacg acagacttga ctcaatggcg ctcagtgact
     4681 gttcaagagg gtgccaaatc atatcttcga gctgaacatg gggctcaaag gcctggctta
     4741 gtgcctcgtg agtcagacgt gcgtagtggc gctgaatcag tcgccacggt actggttgat
     4801 ctagcaaatc agcctttaca ttcggttcct cgctgactac cgtctgcaca attttgccga
     4861 agtccggtcg cacctgacag agcttcaaaa aagtgctggc cgcccggatt tcatttagtt
     4921 cgtgcaaggc aaggctggtg ctgacaagtg tagtgccgtt tggtacagta gccggcaagg
     4981 gcaaaaagac gacagcccct agctctttaa aacttaataa attgtgtcgt tctcgggcca
     5041 ctacgctatc ggctaattcg cgccaacgct tagtatttgg ctgcaccaag gctatggagc
     5101 ggttttcaaa atcgctcggt tttagtcgct tgtattgatc cagccagcgg tgttgccaac
     5161 ttgagttctc gcttaaccat gctgcggcta gcatggtaat cggcgactca tgcttgacga
     5221 atgaatcaag cgagcgatag ccgagttcac gcatagcttt tttgggcggc aacttcttta
     5281 ttagcgtctt taccgcacta cccttgagcg caaagcagcg cttggtatca ggcaattctt
     5341 ggagcgcgtg aaccataccc gccaccactt cgccttcagc ggaaatatga gttgctgcta
     5401 gagttctcag ttttttagtg agccgggcgt cgtccgcttg cactcgttcc tgtaaggcgt
     5461 gatagagctc ctcggccgta gtatcgtgcg gatctaagcc caattggcgg agttttgcct
     5521 gggacgcatg cattacctct gtagagaagc gaatgtcagt actgggatgg ccgtttgccg
     5581 cttctagttt tctgagtcca agccgaaaaa atggttcagg agcctgtaag atctccgata
     5641 aaaaccgtgt catcggctgt ccccaccaaa gttattttta ttttgccgaa gcatataaaa
     5701 tccttttgct tacagattac cataagcgct cgtgttgcga aagcttttac ttcagaggcg
     5761 ttggtttgta ccagtccggc gcttgccgca agaagtgttc aagctctttt tccgtcagta
     5821 agcccgcttg ggcgccgact ttttgcacca cactcatcga gttgatcggc gcccattgca
     5881 aagcgccctc gatagtatta cctttcatga gcgctgccac aaatgtggaa gcgaaggaat
     5941 ctcctgctcc tgtgcggtca accggcggag caggatccgg gtacaagggc atctggaagc
     6001 ggctttgacc gtcagaagcg taggcgccgt tcggtccatc ggtgataacc acaacccggg
     6061 ggcctaaagc atgcaagtgg tcaaacaggt catgaatgtc ctcgtgatta ccgccaccca
     6121 ccagcacagc ttcttcccgg ttcaaaatta atacctcact gcgagcataa atccgctgca
     6181 agcgctcggc gccggcttcc atctggaaag tgccgggttg gaaggcc
//
LOCUS       DATNGP010000099         2358 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_3459278, whole genome shotgun sequence.
ACCESSION   DATNGP010000099 DATNGP010000000
VERSION     DATNGP010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2358
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3459278"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..472
                     /locus_tag="VN778_02440"
     CDS             <1..472
                     /locus_tag="VN778_02440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49861.1"
                     /translation="FMASVKERDQIFYFEDQRVASLFAIMYLNCGRLIWLDPSSHGSY
                     QLLAGAIKRALANKALQHDDFFLTDQEVWDMLQALSDPDIQALLARLQPGREFAYAER
                     SEAEFYASNKTRIIDPLVLANGELKRLSAIILGLRAYFDEYRERYAKIGVRQQG"
     gene            complement(469..1089)
                     /locus_tag="VN778_02445"
     CDS             complement(469..1089)
                     /locus_tag="VN778_02445"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HXR49862.1"
                     /translation="MFNVTHLIEVGGLLALFLIIFSESGMMVGFFFPGDTLLFTSGIL
                     AASGRLPALEVILVVAFAAILGDNVGYHIGRSFGRRLFSKPDSFIFRKAYIERAEKFY
                     EKYGSKTMLLAHFVPIVRTFAPVTAGAGNMPLKKFMLYDAIGDTAWAVAVILAGYFIG
                     SRIPGIENLLEPILLLVVVVSLGPTIYHLARDPKVRQAVDKLFRRS"
     gene            1247..1948
                     /locus_tag="VN778_02450"
     CDS             1247..1948
                     /locus_tag="VN778_02450"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HXR49863.1"
                     /translation="MSEDTLSLELNLREVTGKKVKTLRANGTVPAVIHDHGKPSINVE
                     APYLAIYRTYLHAGKHHPVQLTAGDKKFTALIKSATFEPKKNMLTHVVFNAVNRNQKV
                     EAEVPIHPRYAEGEEASPAELKSLIVLDQLASIEVRAIPDKIPDVLYYDAQKLVEDGD
                     SVAVRDLEIPEGVEIEEEMLDHPVATVTTTAALEAANAAIAGEEEEKPAAETAEGEST
                     EAAAESTETKPEEEK"
     gene            complement(2073..>2358)
                     /locus_tag="VN778_02455"
     CDS             complement(2073..>2358)
                     /locus_tag="VN778_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018561089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXR49864.1"
                     /translation="RAMLKNAPILVLDEATAALDSESEALIQDALWKLMEGRTAIVVA
                     HRLSTIQKMDRIIVMDDGKIIEQGTHKELLHLNGQYAKLWQRQSGGFIEE"
BASE COUNT          625 a          587 c          617 g          529 t
ORIGIN      
        1 gttcatggca tcggtaaaag agcgagacca aatcttttac ttcgaagacc agagagttgc
       61 gagcctgttt gctattatgt atctcaattg cggcaggctt atttggctag acccatcgtc
      121 acacggctcg taccagctcc ttgctggtgc aatcaaaagg gcgctcgcaa ataaagcgtt
      181 gcaacatgat gacttcttcc taactgacca agaagtttgg gatatgttgc aggcgctttc
      241 ggaccccgat atccaggcgc tactcgcccg gttgcagccc ggtcgggagt ttgcctatgc
      301 cgaaaggtcc gaagccgaat tctatgcttc aaacaagacg cgcattatcg acccgcttgt
      361 tttagcaaac ggtgaattga agcggctatc agccattatc ctgggtttgc gagcctactt
      421 tgatgagtac cgcgagcgtt atgccaaaat cggcgtgcga cagcagggct agcttctgcg
      481 aaatagcttg tccacagctt ggcggacctt cgggtcacgg gccaggtggt agatagtagg
      541 tccgagcgat acaacgacta caagcaataa gattggctcg agcagatttt cgatgccggg
      601 aatgcggcta ccgataaaat agccggccaa aatgacagca acggcccagg cggtgtcgcc
      661 gatggcatcg tagagcatga attttttgag cggcatgtta ccagcgccgg cggtaacggg
      721 tgcaaaagta cgaacaattg gcacaaagtg ggcgagtagc atcgttttgc tgccgtattt
      781 ttcgtaaaac ttttcggcgc gctcgatgta ggctttgcgg aaaatgaagc tgtcgggttt
      841 gctgaagagg cgacgcccaa atgagcggcc gatatgataa ccgacgttat cacctaaaat
      901 tgccgcaaag gcgactacca gtatcacttc aagcgctggc aagcgcccgc tggctgccaa
      961 aatacccgag gtaaagagta gggtatcgcc cgggaaaaag aacccgacca tcatgcccga
     1021 ttcagagaag atgattaaaa atagggccaa taagccacca acttcaatca aatgtgtgac
     1081 attaaacata cctccttatc caaccgtatt tgagatgaaa cacgaagtct tgcgtcattc
     1141 ccgcgtatgc aggaatacag gctattttta cttgattccc gatctggccg ggaatgacga
     1201 ccgatggtat actgataagt acaataggag ttaacagagg gaacctatgt ctgaagatac
     1261 attgagtctg gagctcaact tacgcgaagt aacgggtaaa aaggttaaaa cactgcgggc
     1321 taacggtacc gtgccggccg ttatccacga ccacggcaag ccgtcgatca atgtcgaggc
     1381 gccgtatttg gcgatttacc gcacctactt gcatgcaggc aaacaccatc cggtgcagct
     1441 aactgctggt gataagaaat tcaccgcatt gatcaaatca gcgacgtttg agcccaagaa
     1501 aaacatgctg actcacgtcg tgtttaatgc cgtcaatcgt aaccagaagg ttgaggctga
     1561 agtcccaatc cacccgcgct acgctgaggg tgaagaggca agcccggcag aactaaaaag
     1621 cttgatcgtc ctagaccagc ttgcgtcgat tgaagtccgt gcaatacctg acaaaattcc
     1681 agacgttctg tactacgatg cgcagaagct cgttgaagat ggcgatagtg ttgctgtgcg
     1741 cgatctggag attcccgaag gtgtcgaaat tgaagaagaa atgctcgatc acccagtcgc
     1801 taccgtgact accactgccg cgctggaggc ggcgaacgct gccattgcgg gtgaggaaga
     1861 agagaagcct gcagctgaaa ccgccgaggg cgaatcaaca gaagctgctg ctgaaagcac
     1921 cgagaccaag cccgaagaag aaaaatagag ccgccacaat tcgttccgta agtcttaata
     1981 cggttttgct gtgcaggaaa agagagcccg agcaaaagtc acaccttttg cccggcgcga
     2041 tgagagaaac catacggttt ctctcatttg ggttattcct cgatgaaacc gccggactgg
     2101 cgctgccaga gcttggcata ttggccgttt aaatgcagca attctttgtg tgtgccctgc
     2161 tcaatgattt tgccgtcatc catgacgatg atccgatcca ttttctggat cgtgctcaag
     2221 cggtgtgcca ccacgatagc ggtgcgacct tccatcaatt tccacagcgc atcttgaatc
     2281 agcgcttcgc tttccgagtc aagcgcagca gtagcctcat cgagcactaa tattggtgca
     2341 tttttgagca tcgctcga
//
LOCUS       DATNGP010000100         3190 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_2175498, whole genome shotgun sequence.
ACCESSION   DATNGP010000100 DATNGP010000000
VERSION     DATNGP010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3190
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2175498"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            248..1789
                     /locus_tag="VN778_02460"
     CDS             248..1789
                     /locus_tag="VN778_02460"
                     /inference="COORDINATES: protein motif:HMM:NF014147.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 30 beta sandwich
                     domain-containing protein"
                     /protein_id="HXR49865.1"
                     /translation="MPEQSPDNYRHPLTWQGLSRMTAIALAVVSFDASPALAHSLPTH
                     HHRAKTHHIAPVGDVAITQTDPTSDEYMSPLPNQAFVRPAVAHASGNNQVVTVNDKIT
                     YQRFEGIGAAMTDSSAWLIQDELTPANRTQLMSDLFSKSGIHLSFVRIPMGASDFTRN
                     GVPYSYDDMPPGQTDPTLSHFSIAHDRAYILPALHEAEAENPHITFSMNPWSPPGWMK
                     ANDSLDNADGAGVFLPQYNQQFANYEVKSVAAYDRAGVPISDLVPTNEPDVGSLYPGM
                     NFSAQAEGTYISQYLEPTLNAAGLNSVKVYGNDLSWDQTGYANTVLQMVPHLGGLAWH
                     CYTGDPAVMKLYPNVNEIMDECSNELPHFPAALIMAEAFRDNVSIASVWNVALQPGGG
                     PVQPPDKGCPGCFGVATVNPETHTYRYTRRYYQLGQLSHFIMRGAVRLDSQASDEPTF
                     DDTTQAFSPGLVDVAFENPDGTKELVVYNNNASTTTFNVNWNGRVLPNYKVAPGATTT
                     FSWKK"
     gene            complement(1790..>3190)
                     /locus_tag="VN778_02465"
     CDS             complement(1790..>3190)
                     /locus_tag="VN778_02465"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXR49866.1"
                     /translation="AQFMVILDVSIVNVALPAMQRAFHMTASNLQWIITIYTLTFGGF
                     LLLGGRTADLFGRRRTFLAGVIVFTLASFGTGVSQSQAMLIFFRAAQGLAGAFMSPAA
                     LSIVLVTYREGHERNVALSVWGAVASGGAAVGVLLGGILTQYLNWRWNFFINVPVGIF
                     VVLAALRVVGHHKEVAEHNNLDLPGAISVTAGLMALVYALVKAPVYGWTEHRTLTYFA
                     ISFVLLLFFVLNESRVQHPLMPLRIFRIRNLSGADSLMMFQAAGLFSVFFFTTLFLQE
                     ILGYTPVKTGVSFLVIPVAIALTATNVPRVIKRVGYRPILMIAPLVVSAGLFWVSHIT
                     VHSTYWGNVAPGMILMGLGMGATFVSVTIAATSGVPQHETGLASGLLNTSQQIGGALG
                     LAVLTGVATSATGRYLQNLHEAPTQATRAVATVHGFHDGYLIASCFGVAASLLATIVI
                     RAQSTQTDPSHGGPLV"
BASE COUNT          767 a          942 c          825 g          656 t
ORIGIN      
        1 cttgatacgg ctctggtagc tagccggcca agagcacttt ccgctagtgg cgtttctgct
       61 ggttgattta ctgcctgagg tgactcgggc aagctctcca tggtgttcac gtcgtttttc
      121 ccacttcttt tagcattagc gagattatag catattcgta ttaattttac aaatactttt
      181 tatggttgac ctgctgggta ttttatacta tagtattaac tcgtcttgaa gtgtttagtt
      241 aattgacatg cctgagcaaa gccccgataa ttatcgccac cccttgactt ggcagggatt
      301 gagccggatg acagccatcg ccttagccgt tgtcagtttc gatgcctctc cagccctcgc
      361 ccacagcttg ccaacacatc atcaccgagc caaaacgcat cacattgcgc ctgtcggtga
      421 cgtcgcaatc acccagaccg acccgacgag cgacgaatac atgagcccct tgccgaatca
      481 agcttttgtg cggccggcgg tggctcacgc ttctgggaat aatcaggtcg tcactgttaa
      541 cgacaaaatc acctaccagc gtttcgaagg cattggtgct gccatgaccg acagttcggc
      601 ctggctcatc caagacgagc tgacgccggc gaaccgaaca caactgatga gcgatctttt
      661 cagcaaaagc ggtattcact tgagctttgt acgtatcccg atgggggcct ctgactttac
      721 tcgcaatggt gtgccgtata gctacgacga tatgccaccg ggccagaccg atccgacact
      781 cagccatttc tccatcgcgc atgaccgggc ttatatcctg ccggcactgc acgaagctga
      841 agccgaaaac cctcacataa ctttctctat gaacccctgg agtccgcccg gttggatgaa
      901 agctaatgac agcttggata atgctgatgg cgccggtgtt ttcttgccgc aatacaatca
      961 gcaatttgca aattacgaag tgaaatcagt tgccgcctac gatcgggccg gcgtccccat
     1021 tagcgatttg gtgccgacta acgagccgga tgtcggctcc ctgtaccccg gcatgaactt
     1081 cagtgctcag gccgagggca cctatattag ccagtatctg gaacccaccc taaacgccgc
     1141 cggcttgaac agcgtcaagg tctatggcaa cgatctcagc tgggatcaga ccggctacgc
     1201 caataccgtg ctgcaaatgg tgccgcacct gggcggtctg gcctggcact gctacaccgg
     1261 cgacccggcc gtcatgaagc tctatccgaa cgtcaatgaa atcatggacg aatgctcgaa
     1321 tgagctaccg cacttcccgg ctgccctgat tatggccgag gcctttcgcg acaacgtcag
     1381 tattgcttct gtttggaatg tcgctttgca gccgggtggc ggtcccgtcc agccgcccga
     1441 caagggttgc cccggctgct tcggcgtcgc gaccgtcaac cccgagacac acacctaccg
     1501 ctacactagg cgctactacc agctcggcca actcagtcat ttcattatgc gcggtgcggt
     1561 gcgtctcgat tctcaggcca gcgatgagcc cactttcgat gacacgaccc aagctttctc
     1621 tcccggccta gtcgacgtgg ccttcgaaaa tccggatggc accaaggagt tagtggttta
     1681 taacaacaat gcttccacga ctaccttcaa cgtcaactgg aatggccgcg tgttgccaaa
     1741 ctataaagtc gcgcctgggg ccacgactac cttcagctgg aaaaaataac tagacgagcg
     1801 ggccaccatg gctcgggtcg gtctgagtcg actgagcccg aatgacgatg gttgccagca
     1861 acgaagcggc gacaccgaag caggaagcga tgaggtagcc atcgtggaaa ccgtggaccg
     1921 tggccaccgc ccgggttgct tgggtggggg cctcgtggag attctgcaaa taacggcccg
     1981 tggccgaagt cgcgacaccg gtcagtactg ccagcccaag tgccccgcca atctgctgcg
     2041 aggtatttag aagcccggaa gccaggccgg tttcgtgctg cggtacgcca gaggtggcgg
     2101 cgatagtcac gctaacgaag gtcgctccca tcccaagtcc cataagtatc atgccgggtg
     2161 ccacattgcc ccagtaggtg ctgtggaccg taatgtgcga tacccagaac agtccggcgc
     2221 ttactaccaa cggcgcaatc atcaagatcg gccgatagcc tacccgctta atcacgcgcg
     2281 gcacattggt cgccgtcaag gcaatcgcaa ccgggataac caagaagctc acccccgttt
     2341 tgaccggcgt ataacccagt atttcctgca ggaacagcgt ggtgaagaaa aagaccgaga
     2401 ataggccggc agcctggaac atcatcaaag agtcagcccc ggacaggttg cggatccgga
     2461 aaatacgcaa gggcatgagc gggtgctgga cgcggctctc gttcagcaca aagaacagta
     2521 agagtacaaa actgatcgca aaatacgtca gcgtgcggtg ctctgtccaa ccatacaccg
     2581 gtgctttgac gagcgcatac accagcgcca ttagaccagc cgtcaccgaa atggcgccgg
     2641 gcaagtcgag gttgttatgt tcagccactt ctttgtggtg acccacgacc cgcaacgcag
     2701 ccagcaccac aaaaataccg accggtacat tgataaagaa attccagcgc caattcagat
     2761 actgtgtcag aataccgccc agcaatacac cgactgcagc tccgccagag gccaccgcgc
     2821 cccagacgct taaggctaca ttgcgctcat ggccttcgcg gtaggttact aaaacgatcg
     2881 acagcgcggc cggtgacata aaagctcccg ccaggccctg tgcagctcgg aagaaaatca
     2941 acatagcttg tgactggcta acccccgtgc cgaacgatgc tagagtaaaa acgataacac
     3001 cggccagaaa cgtccgacgg cgcccaaata aatctgccgt gcgaccgcca agcagcaaaa
     3061 agccaccgaa ggtcaaagta taaatggtaa taatccactg caaattgctg gccgtcatgt
     3121 gaaaggctcg ctgcatcgct ggcagcgcca cattaacgat tgatacgtcg aggatcacca
     3181 tgaactgcgc
//
LOCUS       DATNGP010000101         5973 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_4450507, whole genome shotgun sequence.
ACCESSION   DATNGP010000101 DATNGP010000000
VERSION     DATNGP010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5973)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5973)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5973
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4450507"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1147)
                     /locus_tag="VN778_02470"
     CDS             complement(<1..1147)
                     /locus_tag="VN778_02470"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HXR49867.1"
                     /translation="MAKATKYVFVTGGVLSGLGKGITAASIGNLLKARGARVNLQKCD
                     PYLNVDAGLLNPREHGECFVTKDGAETDLDLGHYERFVDEEMTSKSSLMSGRVLLKLI
                     QDERAGKFEGDDVQIIPHLTGAIQDWIIKAGEGYDVHIVEIGGTVGDYESLSFIEAIR
                     ELGLKVGLENCAYVHVVYLPYLNASHETKTKPAQNSVRELRGLGIAPDILVGRSEQPA
                     SEAAIKKLSLFSGVPVDAIASLPNARSIYEVPLTLEDTGIADVITDRLGLKAKKPDLK
                     AWRKVVERATAEHTRTVKVGFLAKYTDNTDTYMSVFEALKAAAWSNDCKVDITWVDAT
                     AFDQPGSDITADLKEYDGILVPGGFGQRGLEGKIKAAQYALREKVPYL"
     gene            complement(1211..2440)
                     /locus_tag="VN778_02475"
     CDS             complement(1211..2440)
                     /locus_tag="VN778_02475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HXR49868.1"
                     /translation="MRLKRKQGDSETVVRHRRQWPRRLVSLLLAGVIFACGVGVGNGR
                     INLDLHRTQSVATSLPDKLDYSTVNQVYQSLKENYDGQLTEGQLVDGLKHGLAEATKD
                     PYTEYFTAKEAKDFNDELNNSFSGIGAELGEDSNGNLQIIAPITGTPAAQAGLKAQDI
                     IASINGSTTTGISVDDAVTKIRGPAGSKVTLQVVRSKSQALSFTITRQNITVPSVTTK
                     TLDGNIGYIQISTFADDTNDLIQKAADQFAGAHLKGIILDLRNDPGGLLDAAEHVSSL
                     WLQKGQMILQEKRGNIVEDTAYAPDNGELHGIPTVVLINGGSASASEITTGALHDNHD
                     AYVIGEKSYGKGVVQQLINFGDGSQLKVTVASWYRPDGKNINHLGITPDKTVKEPSTA
                     TPDNDPQLQAAEAYLNK"
     gene            2538..4622
                     /locus_tag="VN778_02480"
     CDS             2538..4622
                     /locus_tag="VN778_02480"
                     /inference="COORDINATES: protein motif:HMM:NF019236.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HXR49869.1"
                     /translation="MKLQHKLFGKKVGAKSLPRFILFASVFGFIGLFTLLYNFAASPA
                     ITYYVATNGSDTNPGTQTQPFQTISKAVSTATGGDTILVNSGVYHEQVNILKSSLTLR
                     GVGPTKPVIDGDNVRGAGINYGSNHLGDETVDNFELRNFYTANTASRATGIVGYEVYS
                     SVFENNDIHDAHGGPGVNGMGIVLGTNSAPYVGQNDKILNNRIHDIGPGGESWGIWLL
                     YTQNMTIDGNSIYMVRKEGVRDWHGLSNTITNNRAFLNWIGIEIETAQKDYIANNYSY
                     NNVFGFSEKHNAGKDSGTATCPIQPDTYDKDVSGNVDYSNWSRFWHNTAYHNTGTGFA
                     LGQSGPQGDYIAIEDNIFAGNGNNGVWNWPSTTCGHVILDGNVYSRLAPPNHPYWDYY
                     AGWNSPPSDLSQTVTDINNLLYNGGKTTADGWGWAGWFVAGGPEPKINFEQHGQDFNM
                     TFKDPGHADLDYTTAPPNAGLSLDDSYGSQLGARNVPAETVQWTEYPMTAIAASAGGI
                     SSNFGSAARTTDDTDNTYWWSSGAPNANTNQWITYDLGQARTFQQFVVSVFSQYDSRF
                     VKNYSFQVSNDDQTWTTVLAGTNPDDEGSSYKYELPQPVTARYIKFNMVNNFGGAYLI
                     LPDVQVGLLQNANTAPITIVGDLNGDGHVNIFDLSLLLAKWNKADAAADLNHDGIVNI
                     FDLSLLLGNYGI"
     gene            complement(4651..5778)
                     /locus_tag="VN778_02485"
     CDS             complement(4651..5778)
                     /locus_tag="VN778_02485"
                     /inference="COORDINATES: protein motif:HMM:NF017102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HXR49870.1"
                     /translation="MPKLTSVKTTFYRLRALALVLVAGGVVGGAALVHADQFDAQINA
                     LQQQNAAAQNVLNGLTSQASSYQGAIDQLQAQINAVQGQIAANQAQQAQVQQQITTDQ
                     QQIDQKKQQLSATIKAMYIDGQTSTIEELASSKNLSDYVDKEEYRTNVQDQLNGKIKE
                     IADLQAQLQRQKSDLDTLIASEKTQNDQLGADQAQQNQLLAYNEGQQSAYNQQISANS
                     GQIAELRREQAVLNARYNIGNFKGDPGNGGYPSAWAYAPQDSMIDSWGMYNRECVSYT
                     AFKVHQDYLSGRDSHDMPWWGGSGNANEWPGDARAAGIPVDSNPTPGSIAISTAGFFG
                     HAMYVEAVNGNEIYVQQYNQQLTGQYSEGWRYTTGLVFLHF"
BASE COUNT         1328 a         1542 c         1546 g         1557 t
ORIGIN      
        1 cgagatacgg tactttttcg cgcaaagcgt attgcgcggc tttgatcttg ccttccaggc
       61 cgcgctgacc gaagccgccg gggactaaaa tgccatcgta ttctttgaga tcggcggtaa
      121 tgtcgctgcc cggttggtcg aaggccgtgg cgtccaccca agtgatgtca actttgcaat
      181 cattagacca cgccgcagct ttcagtgctt caaacaccga catataggta tcggtattgt
      241 cggtgtactt cgccaaaaaa ccgactttta ccgtccgtgt atgctcggcg gtggcccgct
      301 cgacaacttt gcgccaggct ttgagatctg gcttcttggc ttttagcccg agtctgtcgg
      361 taatgacatc ggcgatgccg gtatcttcga gcgtgagcgg tacttcgtaa atactgcggg
      421 cattcggcaa gctggcaata gcatcgacag gcacaccgct aaacaggctc agcttcttga
      481 tggctgcctc agaagccggt tgctcgctgc ggccgactaa aatatccggc gcaataccta
      541 agccgcgtag ctcgcgcacc gagttttggg ccggcttagt tttcgtttcg tggctggcat
      601 ttagatacgg cagatacact acgtgtacat aagcgcagtt ttccaagccg actttcaacc
      661 ccagctcgcg aatggcttcg ataaatgaca gcgactcgta gtcgccaacg gtgccaccga
      721 tctcgacgat atgcacatcg taaccctcgc cggctttgat aatccaatcc tgaatggcgc
      781 ccgtcaggtg tgggatgatc tggacgtcat cgccttcaaa ctttccagct cgctcgtctt
      841 ggattagttt gagtaaaacc cggccactca tcagagagct cttggaggtc atctcttcgt
      901 cgacaaaacg ctcataatga ccaaggtcga gatctgtttc cgcgccatct ttggtgacga
      961 agcactcgcc atgctcacgc ggattgagca ggcccgcatc aacgttaaga tacgggtcgc
     1021 atttctggag attgacacgt gcgccgcggg cttttaagag gttaccgatt gaggcggcgg
     1081 tgataccctt ccctaacccg gaaagcacgc cacctgttac aaacacatac ttggtcgctt
     1141 ttgccacgac taaccctacc tcttgttatt gctctaatgc taccctatta ctcctctgtt
     1201 ggcaagcctt ctatttgttg agataggctt cggcggcctg gagctgagga tcgttgtcag
     1261 gtgtagcggt ggatggttcc ttgacagttt tgtcgggagt gatgccgaga tgattgatgt
     1321 ttttaccatc gggacgatac cagctggcga cagtgacttt gagctgcgag ccgtcaccaa
     1381 agttaatgag ctgttggaca acgcctttgc cgtagctctt ttcgccgatg acgtaggcgt
     1441 cgtggttgtc gtgtaaagcg ccggtggtga tttctgaagc cgaggcgctg ccgccgttga
     1501 tcaggactac cgttgggatg ccgtgcagct caccattgtc cggagcataa gcggtatctt
     1561 cgacaatgtt gccgcgtttt tcttgcaaga tcatttgacc cttctggagc cataagctgg
     1621 aaacgtgctc ggccgcatcg agtagaccac cggggtcatt cctaaggtcg agaatgatgc
     1681 ctttgaggtg ggcaccggcg aactggtcgg ccgctttttg tatcagatcg ttcgtgtcgt
     1741 cggcaaaagt actaatttgg atgtaaccga tgttgccgtc gagggtcttg gtggtgacac
     1801 tcggcacagt aatgttttgg cgggtaattg tgaagctcag tgcctgagat ttgctgcgca
     1861 cgacctggag cgtcaccttg ctgccggccg gaccgcggat ttttgttacc gcgtcatcga
     1921 cgcttatgcc tgtggtcgtg gagccgttaa tactggcaat gatgtcctga gctttcaagc
     1981 cggcttgggc cgcgggcgtg ccggtaatcg gggcgataat ctgtaagttg ccgttgctgt
     2041 cttcgccgag ttcggcgccg atgccgctaa aggagttatt aagctcatcg ttgaaatcct
     2101 tagcctcttt ggccgtaaag tactcggtgt atggatcttt ggtggcttct gccaggccgt
     2161 gcttgagccc gtcgacgagc tgtccttcgg taagttgacc gtcataattt tccttaagtg
     2221 actggtagac ttggttaaca gtggagtagt cgagcttgtc cggcaagctg gtggcgactg
     2281 actgcgtgcg gtgcaagtcc agattgatcc gtccattgcc cacgcccacg ccacaggcga
     2341 aaatcactcc tgccaacagc aggctgacaa gccgcctcgg ccactgtcgc cgatgccgaa
     2401 ccacagtctc actatccccc tgtttgcgtt ttagtctcat tgctactcat tatagcaaac
     2461 ggttagcctc tttctatgct acaattccgc tagaggttta gggcaaaatg agagcaaaaa
     2521 ctcatgctca aagacttatg aaattacaac acaaactgtt cggtaaaaag gtgggggcga
     2581 aaagtcttcc ccgcttcatt ttatttgctt ctgtgtttgg ttttattggt cttttcacgc
     2641 ttctttataa ttttgcggcc tcgccagcga tcacctacta cgtcgccacc aacggctcgg
     2701 acaccaatcc aggtacccag acacagccat ttcagacaat cagcaaagct gtttctacgg
     2761 caaccggtgg tgataccata ctagtgaatt ccggggttta ccacgagcag gttaacatcc
     2821 tcaagagtag cctaacgctc cgaggtgtcg gcccaaccaa gccggttatt gatggtgaca
     2881 acgttcgtgg agccggcata aactatggct cgaaccacct cggcgacgaa acagtggata
     2941 attttgagct gcggaatttc tacaccgcca ataccgcctc gcgcgctacc ggtatcgtgg
     3001 gttatgaagt gtatagttcc gtctttgaga ataacgacat tcatgacgct catggtggtc
     3061 cgggcgtcaa cggcatgggt atcgtacttg gcacgaacag cgccccttac gttgggcaaa
     3121 acgataaaat cctgaataac cggattcacg acatcggccc gggcggtgaa tcatggggta
     3181 tttggctgct ctacacgcaa aacatgacca tcgacggcaa ttctatttat atggtacgta
     3241 aggaaggagt acgggattgg catggtctga gcaacacgat caccaacaac cgcgcttttt
     3301 tgaactggat aggtatcgag atagaaacgg cacaaaagga ttatattgcc aacaactatt
     3361 cttataataa tgtgtttggc ttttccgaaa agcataacgc cggcaaagac agtggaactg
     3421 ccacctgccc catccagcca gacacctatg ataaagacgt cagtggcaac gtggattata
     3481 gtaactggag ccggttttgg cacaacaccg cttatcacaa tactggaact ggttttgccc
     3541 ttggtcaaag cgggccgcaa ggcgactaca tagccattga ggataatatt tttgccggca
     3601 atggcaacaa cggcgtctgg aattggccca gcacgacctg tggacatgtg attttggatg
     3661 gcaatgttta ttctcgcctg gccccgccta atcatcctta ctgggactac tacgcgggct
     3721 ggaattcccc accttcagat ttatcacaaa ccgtcacgga cattaataat ttgctttaca
     3781 acggcggtaa aaccactgcc gatggctggg gatgggccgg ctggtttgtg gccggcgggc
     3841 cagaaccaaa aattaacttt gagcagcacg gccaggactt taacatgaca ttcaaagatc
     3901 ccggccacgc cgatctggat tacaccacgg ctccgcccaa tgctgggcta tctctcgatg
     3961 atagttacgg gtcgcagtta ggcgcgcgaa atgttccagc cgaaaccgtg caatggactg
     4021 aataccccat gaccgctatt gctgcttccg ccggcggcat ttcatccaac ttcggcagtg
     4081 ccgcccggac taccgatgat accgataaca cctactggtg gtcatccggc gcccccaatg
     4141 ctaatactaa tcagtggatc acctacgacc tcggccaggc ccggactttt cagcaatttg
     4201 tggttagtgt atttagccag tacgacagtc ggttcgtaaa aaattactcc ttccaggtat
     4261 cgaatgacga ccaaacctgg acaacggtgc tagcgggcac caaccctgac gacgaaggct
     4321 cgtcatataa atatgaactg ccgcaaccgg tcaccgcccg ctatataaag tttaatatgg
     4381 tcaataactt cgggggagct tatctcattc tgccggatgt tcaagtcggg ctactccaga
     4441 atgctaatac agcgccgatc acaatagttg gcgacctcaa cggcgacggc cacgttaaca
     4501 tctttgattt aagcttactc ctcgcgaaat ggaataaggc cgacgccgcc gccgacctta
     4561 accatgacgg tatagtcaat atcttcgact tgtcgctgtt gcttgggaat tacggaatct
     4621 agtctttcta gtcccgctag tcgcaggttt ttagaagtgt aggaacacta agccggtagt
     4681 gtagcgccag ccttcggaat attgaccggt cagttgctgg ttatattgct gcacatagat
     4741 ttcgttgcca ttgacggctt cgacatacat agcgtgaccg aaaaagccgg cagtactaat
     4801 ggcgatggag ccaggtgttg ggttgctgtc gaccggtatg ccggccgccc gggcatcgcc
     4861 cggccattcg ttagcattgc cagaaccacc ccaccatggc atgtcgtgac tgtcgcgacc
     4921 ggacaaataa tcttggtgaa ctttgaaggc cgtgtagctg acacactcgc ggttgtacat
     4981 accccagcta tcgatcatgc tgtcctgcgg cgcgtaagcc caggcacttg ggtagccgcc
     5041 attgccggga tcacccttga aattaccgat gttgtagcga gcatttaaga ccgcctgttc
     5101 gcggcgcaat tcggcaattt gaccggagtt ggctgaaatt tgctgattgt aggctgactg
     5161 ctggccttcg ttgtatgcta gtagctgatt ttgctgggct tgatcggcgc ctagttgatc
     5221 attctgggtt ttttcggagg caatcaacgt atccaggtca ctcttttgtc gctgtagttg
     5281 ggcctgtaag tcggcaatct ctttgatttt accattgagc tgatcctgaa cattggttcg
     5341 atactcttcc ttgtcaacat aatcgctcaa gtttttacta ctggccagct cctcgatggt
     5401 gctggtttga ccatcgatgt acatagcctt gatagtggcc gatagctgtt gcttcttctg
     5461 gtcgatctgt tgctgatcag tggtaatttg ctgctgtact tgagcctgct gagcttgatt
     5521 ggcagctatc tgaccctgta cggcattaat ttgagcttgt agctggtcga ttgcgccctg
     5581 gtagctgctg gcctggctcg tcaaaccatt caagacgttc tgggcagcag cattttgctg
     5641 ctgcagcgca ttaatttgag cgtcgaattg atcagcatga actagcgctg caccacccac
     5701 tacaccacct gcgaccaaca ctaaagcgag ggcgcgcagc ctataaaaag tagttttgac
     5761 cgatgttagt tttggcatag ttttgttttt gcttatgcta ggtcgtattg taccactctt
     5821 gtgcaagatt gtcattaccc tcccctatac agttgatgtt atgagactaa aagagagggc
     5881 ttgtaccctc gtcgtaatta gtttttctat ttcgtcttga atttgaggta gcggcgggtc
     5941 gcgatggtag atgataccgc tccaagtact ata
//
LOCUS       DATNGP010000102         5033 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_587090, whole genome shotgun sequence.
ACCESSION   DATNGP010000102 DATNGP010000000
VERSION     DATNGP010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5033
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_587090"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..781
                     /gene="atpD"
                     /locus_tag="VN778_02490"
     CDS             <1..781
                     /gene="atpD"
                     /locus_tag="VN778_02490"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013279143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HXR49871.1"
                     /translation="TSLVFGQMNEPPGARLRVGLSGLTIAESFRDQGNDVLLFIDNIF
                     RFTQAGAEVSALLGRLPSAVGYQPTLQQEMGALQERITSTKEGSITSVQAVYVPADDF
                     TDPAPATTFAHLDSTIALNRALTEIGIYPAVDPLDSTSTILDPEIVGEDHYNVAREVQ
                     RVLQRYKDLQDIIAILGMEELSEEDKQLVGRARRIQRFLSQPFFVAEQFTGNSGQYVK
                     LTDTVQGFKEILEGKHDDKPESAFYMKGGIADVNKAAKGAK"
     gene            784..1215
                     /gene="atpC"
                     /locus_tag="VN778_02495"
     CDS             784..1215
                     /gene="atpC"
                     /locus_tag="VN778_02495"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008578630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HXR49872.1"
                     /translation="MFHFQLVSISGKKFDEDVYEVVLPTLDGQIGVMQDHMPLVSVAA
                     PGAIMVRRNPRDPDHAREFFATSGGAIEVSNNTLRVLVDEADRAEEINETEAQKALEL
                     ATKMKTEAKDQVSLEHAQQLVDRHEVRLQVAGLKRRHQRNR"
     gene            1218..1475
                     /locus_tag="VN778_02500"
     CDS             1218..1475
                     /locus_tag="VN778_02500"
                     /inference="COORDINATES: protein motif:HMM:NF019481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1653 domain-containing protein"
                     /protein_id="HXR49873.1"
                     /translation="MSKQKSIDEILAGWEGLPRPQEGDTYRHYKGGQYVIVATGFLED
                     TEAPCVVYKSLEKNITWVRTAKNFLETIEHNGQTTARFTKI"
     gene            complement(1472..1885)
                     /gene="mscL"
                     /locus_tag="VN778_02505"
     CDS             complement(1472..1885)
                     /gene="mscL"
                     /locus_tag="VN778_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018655900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HXR49874.1"
                     /translation="MLNDFKKFILRGNVVDLAVAVVIGAAFNSVVQSLVKDFITPLIT
                     GVSKKGSLVQGQFTVDHRFIFNYGDFLNTLISFVIVAAVVFFFVVQPINKLNELAARR
                     KGTEEPTTKKCPECLSEISKKATRCAFCASKLKAA"
     gene            complement(1953..2267)
                     /locus_tag="VN778_02510"
     CDS             complement(1953..2267)
                     /locus_tag="VN778_02510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49875.1"
                     /translation="MRKRHEGKQNPPLTNQEALNTLFVAEHHPSHDQKWSRLRSDIAR
                     DALIKAQSLRERNVDAGDAYLQGVADLYHRLERDRLVDEVVQCFDPNAELSRLTDEPK
                     TD"
     gene            2424..3365
                     /locus_tag="VN778_02515"
     CDS             2424..3365
                     /locus_tag="VN778_02515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49876.1"
                     /translation="MHEAFTQGEKLMNLEQIQIAKIATLDFGQGKVGENVKLVGPEAE
                     TQFAGALHAHTHELTIYNTADCGDGRRTIRLASGEEDAVVLRERVVSQLFGGLGLATT
                     KALVAANARLVSDATSFWQAYETVSTTLANINKGDVKYEDAGHESCGASGSVEASVAN
                     KITPELLIPSIGLFIPMNETVEAQVTQNATNKQHRLEAGFYSGWDPQKHADYLISRFP
                     HNFSYLEVDQDDPVTHGHNEAGVYVVTDENQGFAKNEFIEDTGRQAFAVTFRTMQEIA
                     DKLGGSDEERMAILLGFIDDTLHAGAGLVMPGMPVFA"
     gene            3439..4173
                     /locus_tag="VN778_02520"
     CDS             3439..4173
                     /locus_tag="VN778_02520"
                     /inference="COORDINATES: protein motif:HMM:NF012455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HXR49877.1"
                     /translation="MFGREKIATLVAEFLGTGILTLLVLSVQRSTIGVPFFVAAAAGL
                     TVILMVFALGGISGAQLNPAVTLAMWTVRKITALTGVLYIIMQFLGAWAAYGLYHYYV
                     KSSFQPIGGHFTGRILVAEAVGTGIFVFGLAAAVYQAYTRAVTATVYGLAYMIGIIAA
                     SSAAIGLLNPALAFGVRAFVPWGSGVAGWGTYFLGPILGAIIGANLYSLLFAVPEAVV
                     AAPAAASSRAITTKKPAAKRAGSRKK"
     gene            complement(4221..>5033)
                     /locus_tag="VN778_02525"
     CDS             complement(4221..>5033)
                     /locus_tag="VN778_02525"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HXR49878.1"
                     /translation="LKNYKLSVTHLNNFIDLRDAGPQYFLVHNLLRFPEAMAPAAAYG
                     DAVHKTLQWAYMQFRQSEQLTGMATALTYFDDMLARKHLRESDYIRLEGRGHEALRRY
                     LKQRSKTFKASDIIERGFNNEGVVVNGAHLSGKIDKLRLQDGRAIVTDFKTGKPARTW
                     QGKDEYERLKLHKYRQQLLFYKLLVEYSASYSKRATVQAGALEFIEPDEQGQLIDNLE
                     LDFQPQELQHFVHLIGAVWQHIMSLQFPDVSQYPATTKGVMQFEQDLIDGKI"
BASE COUNT         1228 a         1265 c         1271 g         1269 t
ORIGIN      
        1 cacctcgctg gtcttcggcc agatgaacga accgccgggc gctcgtttgc gcgtcggctt
       61 atctggcttg acgattgctg aaagtttccg tgaccaaggc aacgacgtgc tgctcttcat
      121 cgataatatt ttccgcttca cccaagccgg tgcggaagtc tccgccctgc tcggccgttt
      181 gccgtcagcc gtcggttacc agccgacttt gcagcaagaa atgggtgcct tgcaggagcg
      241 catcacttcg accaaagaag gttccatcac ctcggtccag gctgtctacg tgcctgcgga
      301 cgactttacc gacccggcgc cggcgactac ctttgctcac cttgactcga ccattgccct
      361 gaaccgggca ctgaccgaga tcggcattta tccggctgtc gacccgctcg actctacctc
      421 aaccattttg gacccggaaa ttgtcggcga agaccactac aatgtcgccc gtgaagttca
      481 gcgggtactg cagcgctata aggacttaca ggatattatc gccatcttag gcatggaaga
      541 gctctccgaa gaagataagc agctggttgg acgggctcga cgtattcaac gtttcttgag
      601 ccagccattt ttcgtcgctg agcagtttac cggtaattcg ggccaatacg ttaagctaac
      661 cgacacggta caaggtttca aagaaatcct cgagggcaaa cacgatgata agccagaaag
      721 cgccttctat atgaagggcg gcattgccga cgtcaacaag gcggccaaag gcgccaagta
      781 agcatgtttc atttccagct cgttagtatc agtggcaaaa agtttgacga agacgtctat
      841 gaagtggttt tgcctacttt ggatggtcaa attggcgtca tgcaagacca tatgccactt
      901 gtcagcgtag ctgctcccgg tgctatcatg gtgcgccgca acccgcgcga tcctgaccat
      961 gcccgcgaat tcttcgcgac cagtggtgga gctatagaag tttcaaataa cacacttcgg
     1021 gttttggtag atgaagccga ccgagccgaa gaaattaatg aaaccgaagc ccaaaaagct
     1081 ctcgagctag ctactaaaat gaagaccgaa gctaaagacc aggtgagtct ggagcacgcc
     1141 cagcaactcg tggaccgcca tgaagttcgc ttgcaagtag caggcctcaa gcgccgtcat
     1201 cagcgaaatc gctaaatatg agcaagcaga aatcgatcga cgaaatcctg gcaggttggg
     1261 aaggtttgcc tcgcccgcaa gagggcgaca cctaccgaca ttacaagggt ggacaatatg
     1321 tcattgtggc caccggtttt ctcgaggata ccgaggcgcc ctgtgtcgtc tacaaatcgc
     1381 tcgaaaaaaa tattacttgg gtacgaacag ccaaaaattt tctcgaaaca atcgagcata
     1441 acggccaaac cacggcccgc ttcaccaaaa tctaagcggc tttaagcttg ctcgcacaaa
     1501 aggcacagcg tgtcgccttt ttagaaattt cactgagaca ctccgggcac ttcttcgtag
     1561 ttggctcttc tgtccctttt cggcgggctg ctagttcatt caacttgttt attggctgaa
     1621 ccacgaagaa gaatacgact gccgccacaa taacgaatga aatcaaggta ttgaggaagt
     1681 cgccgtaatt aaagataaac cggtgatcca cagtgaactg gccctgtaca agactgcctt
     1741 tcttactgac gccagtgatc agtggcgtga tgaagtcttt gaccagcgac tggacgaccg
     1801 agttaaaggc ggcgccaatt acgaccgcta cggccaggtc aaccacattg ccgcgaagga
     1861 tgaacttttt gaagtcgtta agcatagtac tttccttatc tctattatgt gatgctactc
     1921 atataataga gcgccggcga gaagagtcaa tgttaatcag ttttcggttc gtcagtcagt
     1981 cttgataatt ctgcgttggg gtcaaagcac tgcactactt catctaccaa acgatcacgt
     2041 tctaagcggt gatagagatc ggctacaccc tgcaaatagg catccccggc atcaacatta
     2101 cgctcgcgaa gagactgtgc ctttataaga gcgtcccgtg caatatcaga gcgcaacctg
     2161 ctccatttct ggtcgtgtga aggatgatgc tcggctacaa ataaggtgtt cagtgcctct
     2221 tgattagtta aaggagggtt ttgcttgccc tcgtgccttt tacgcataca cccctccttt
     2281 ttagtctccc tatgatacag gaaaactgag aggcgccttt gtgataaatc ttaccgagta
     2341 tttgtgtctt gcatcacact tgaatcggga gtactatacc taattggtat taattaacag
     2401 attggtaacg agcaataata ggtatgcatg aagcatttac tcaaggcgaa aaattgatga
     2461 acctcgaaca aatacaaata gcaaaaatcg caaccctgga cttcggccaa ggcaaagtcg
     2521 gtgaaaatgt gaaactagta ggccccgaag cagaaaccca gtttgctgga gccctccatg
     2581 cccatacgca tgagcttact atatacaata cggctgactg cggagacgga cggcgaacaa
     2641 tccgattggc aagcggagag gaagatgcag tcgttctccg agagcgcgtg gtgtctcaac
     2701 tatttggtgg gcttggtctc gctaccacca aagcactggt ggctgccaat gctaggctgg
     2761 tgagcgatgc aacgtcattc tggcaagctt atgagacggt ttccaccacc ctagcaaata
     2821 ttaacaaggg tgatgtgaag tatgaagacg ctggccatga gagctgtggt gccagcggca
     2881 gtgtcgaagc ctcggtggct aacaaaatta ctccggaatt attgatcccc agtatcggct
     2941 tgtttatacc catgaatgaa acggttgaag cgcaagttac gcaaaatgcc accaacaaac
     3001 agcatcgctt agaagccggg ttttatagcg gttgggatcc gcaaaaacat gccgattatt
     3061 tgattagccg ctttccgcat aacttttctt atctggaagt tgatcaggac gatcccgtaa
     3121 cgcatggcca taatgaagct ggtgtctatg tcgtgactga tgagaaccaa ggtttcgcta
     3181 aaaatgagtt tatagaagac acgggccggc aagcctttgc tgttaccttc agaactatgc
     3241 aggagatagc tgacaaactt ggtggtagtg acgaagagcg aatggctatt ttacttggct
     3301 ttattgacga tacactgcat gcgggcgccg gactggtaat gcctggcatg cccgttttcg
     3361 cctgataatc gctgacggct ttacaaaatc ttaaagtcac ggtacgctaa tgacatcaat
     3421 aagtggaggt tctgagtaat gtttggaaga gaaaagatag ctactctggt ggctgagttt
     3481 ctgggtaccg ggattctgac gctcctggtg ctgagcgtgc aacgctcgac tatcggtgtg
     3541 ccgtttttcg tggcagcggc ggctggttta acggtcatcc tgatggtctt tgccttgggt
     3601 ggcatttcgg gcgcacagct gaacccggct gttaccctgg ccatgtggac ggtgcgcaag
     3661 attacggctc tgaccggtgt cttgtacatc atcatgcagt tcttaggtgc ttgggcggcc
     3721 tatggactct atcactacta cgtaaagtct tctttccagc caatcggcgg gcactttact
     3781 ggccgtatcc tggtagctga agctgtcggt accggcatct ttgttttcgg gctggcagca
     3841 gcggtctatc aagcctatac gcgtgccgtt accgccaccg tctatggctt ggcttacatg
     3901 atcggtatca ttgccgcttc ctcggctgct atcggtttgc tgaatcctgc tctagccttt
     3961 ggcgttcgcg cctttgtgcc gtggggctcc ggtgtcgctg gttggggaac atacttcttg
     4021 ggcccaatct taggtgccat catcggtgct aatctgtaca gcttgctgtt tgcagtacca
     4081 gaagctgtgg ttgcagctcc ggccgccgct tcatcgcgcg ctatcacaac caaaaaaccg
     4141 gccgccaaaa gagccggtag ccgtaagaaa tagtcagatt tcttctcagg ctttaagccg
     4201 cccgctagcc gggcggtttt ttatatcttg ccgtcaataa ggtcttgctc gaactgcatg
     4261 acgcctttag tagtggctgg atactgactg acgtcgggaa actgcagact catgatgtgc
     4321 tgccacacgg caccaataag atgcacaaag tgttgtagct cctggggctg gaaatcgagc
     4381 tcaagattgt caatcaactg gccctgttcg tccggttcaa taaattccaa agcacctgct
     4441 tggacggtcg ctcgtttgct gtagctggcc gaatactcaa caagtaattt ataaaaaagt
     4501 aattgttgcc gatatttgtg tagcttcaat cgttcatact catctttacc ttgccatgtg
     4561 cgagccggtt tgccggtttt aaaatctgtg acgatagctc tcccatcttg taatctcagt
     4621 ttgtctatct tgccactgag gtgcgcgcca tttacgacca cgccttcatt attaaagccg
     4681 cgttcgataa tatcgctagc cttgaacgtt ttgctccgtt gctttaggta gcggcgtaaa
     4741 gcttcgtgcc ccctgccctc caagcggatg taatcagatt ctcttaagtg ctttcgtgcc
     4801 aacatgtcat caaaataagt cagagcagta gccatgccag tcagctgttc actttgtcga
     4861 aactgcatgt aggcccattg cagcgtctta tgcacggcat cgccgtacgc ggctgcgggg
     4921 gccatggctt ctgggaagcg cagcaaatta tgcaccagaa aatactgcgg cccggcatct
     4981 cgcaagtcaa taaaattatt gagatgtgtg acactcagct tgtagttctt gag
//
LOCUS       DATNGP010000103         4878 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_1254726, whole genome shotgun sequence.
ACCESSION   DATNGP010000103 DATNGP010000000
VERSION     DATNGP010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4878
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1254726"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            162..704
                     /locus_tag="VN778_02530"
     CDS             162..704
                     /locus_tag="VN778_02530"
                     /inference="COORDINATES: protein motif:HMM:NF015674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HXR49879.1"
                     /translation="MSTASITKENWWALVLGGVATLIFGLAVVFWPGLTVTTLLYLFS
                     AYVLISGVVNVMGSINTVGVVDAWFLPTILGAFELGVGVYLLRHVAVKFTTFVLLIGL
                     VLIVRGVVEAVGAYYVPARAVVARVQVMSYVSGLAAIVAGIVILFSKASAGVSFVWIL
                     GLYAIVVGILHVTQLSNGNR"
     gene            complement(789..926)
                     /gene="rpmH"
                     /locus_tag="VN778_02535"
     CDS             complement(789..926)
                     /gene="rpmH"
                     /locus_tag="VN778_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006805377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HXR49880.1"
                     /translation="MPKRTHQPKKRQRAHEHGFMKRMASRAGRNVLKRRRLKGRAKLS
                     A"
     gene            1175..2626
                     /gene="dnaA"
                     /locus_tag="VN778_02540"
     CDS             1175..2626
                     /gene="dnaA"
                     /locus_tag="VN778_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010164722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HXR49881.1"
                     /translation="MENRAFVEYISLHTWESQVQQDSLWQAVLGEIELSVSRGNFVTW
                     FKNTCLLKQTEDSVVIGVPNVFVKNQLEKKYGDLIKDTLGKNGVSVKQIDYKMAAASA
                     LPRQTPEAAPEILNPQPAVTSSATASRQQAVSKPAGNSLVHSYRQGLNERYTFDNFIV
                     GASNELAFAACQAVSQQPGTRYNPLFLYGGVGIGKTHLMQAVGNILAIRNPDARVLYI
                     TTEQFVQEFVDALRFRKTADFTRHYRTADILIVDDFQFIAGKEKVQEEFFHTFNALHQ
                     ANKQIIISADKPPKDIPTLEDRLRSRFNGGMSIDMQIPDFETRCAIVKNKAQNHDTEL
                     PIDVVEYLAANVQTNIRELEGALNQLLAFCEMRALEPSLPIVSSLLSVSKARPKHLSA
                     KQIIERTARHFQIPLEDILGPRRDKDIVVPRQIAMYMLRSELHLSFPKIARELGRKDH
                     TTAIHSVDKIEKEARVDSSIRMAIADIKEHLYA"
     gene            2906..3997
                     /gene="dnaN"
                     /locus_tag="VN778_02545"
     CDS             2906..3997
                     /gene="dnaN"
                     /locus_tag="VN778_02545"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008578475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HXR49882.1"
                     /translation="MKLQVTQENLNRALSSVARVANTRGTLPILANVLIKTSNNRLSI
                     SATNLDIAITHYIGAKVSEEGSITVPARLMQDFVGSLPEGVIELDLKDTKLHVTTEQY
                     NSVVNGIVADDFPVMPTISKGSKWTVDGAIFKRGLQQVVFAASSDETRPVLTGVLLQT
                     MDGKLFMAATDSYRLTEKQLGANKETVQLLIPASAMQDLLRVLGDGEEKVQVTHDDQQ
                     VLFQVGDAELVTRLVEGNYPDYRKLIPEKFATQATCKRADFLNVTKVSSLFARESAGS
                     VTIEIDEKSSQLSIRSVASQLGENTATAKAKVTGSGSITLNSRYLLDALGALHGEEVV
                     FGFNGKLEPTLLHDPTSADYRHVIMPLKS"
     gene            4017..>4878
                     /gene="recF"
                     /locus_tag="VN778_02550"
     CDS             4017..>4878
                     /gene="recF"
                     /locus_tag="VN778_02550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HXR49883.1"
                     /translation="MLADLHLQHFRSYKDDSFEFSPGVNIVVGPNASGKTNLLEAVLV
                     LARGNSYRAKDGELVQFDHSWARLDTHLLSGSERTIKLSPEATPAKLYELDGKSYKRL
                     TLNHTLPVVLFEPNHLQLLSGSPDRRRDYLDDLLEQTVVGYGNLRRQYKRALAQRNAL
                     LKQPGISTQLFPWNVRLSELAGQIVKQRSQLVTQLAKAVTDLYKDLSHTKTRVSLQYE
                     CPWPQGSYESQLLKKLEMNAELDSQRGFTSAGPHREDLSVLFDGRPSQETASRGEVRT
                     AVLALKITELK"
BASE COUNT         1212 a         1286 c         1218 g         1162 t
ORIGIN      
        1 gtgccgtcga gaaaacatat gtctagcata tagcagatgc ttgcgggcgg gcaattacac
       61 ctctagttcg gcaacttatc cacacatatc catataagcg gattgacggt cgcgctattt
      121 gagtgcatga tgaggatacc aactaaggag ggctctctat tatgagcaca gcaagtatca
      181 ccaaagagaa ctggtgggct ttggttttgg gtggagtagc aacattaatt tttggtttgg
      241 cggtggtctt ctggccgggc ctgaccgtaa ccacactgct gtatctgttc agtgcatacg
      301 tactgatcag tggcgtcgtg aacgtcatgg gcagcatcaa caccgttggt gtggttgacg
      361 cctggttcct gccgaccatt ctcggcgcct tcgagcttgg cgtcggcgtg tatctgctgc
      421 gtcacgtggc agtcaagttc acgaccttcg tgctgttgat cggcctagtg ctgatcgtcc
      481 gcggcgtcgt cgaagcggtc ggtgcatact atgtgcccgc ccgcgcagtc gtagcccggg
      541 tccaggttat gagctatgtc agcggcctgg cggcaattgt agctggcatc gtcatcctgt
      601 tcagcaaagc ctcagcaggt gtgtcgttcg tgtggatcct cggcctgtac gccatcgtcg
      661 tcggtatctt acacgttact cagctgtcta acggcaaccg gtaatacttg ggtttatcga
      721 gtaaaaacca aggcgaaata gaaatacagg tgacaccacc agtcacctgt atttttgttg
      781 gatacaattt aggctgaaag cttggcgcgg cctttgaggc ggcggcgctt gagtacattg
      841 cggccggctc tggaagccat gcgcttcatg aagccgtgtt cgtgggcgcg ctggcgtttt
      901 ttgggttgat gggttcgctt gggcatcaaa aatcctttag tggaattgtc attaactcct
      961 agtgactata tctgttgcgg cccgttttgt caaagagggc ttattagcgc ggactgcctt
     1021 gtagttttga caatgattag ccgctaaccg cctcgagtta tccactaaac ctttaatatt
     1081 atccacaaat taacagatag ggccccaggg tgtgtataaa tcgctatctg tcgccgtgtt
     1141 tttatcgaac cgccgaaaat gaagtcacgg gggtgtggaa aaccgggcgt ttgtcgagta
     1201 tattagtcta catacatggg agtctcaggt gcagcaagat agtttgtggc aagcagtatt
     1261 aggagaaata gaactctcgg tttcacgagg gaactttgta acctggttta aaaatacttg
     1321 tctgttaaaa cagacggagg attcagtcgt tatcggtgtt ccgaatgtat tcgttaaaaa
     1381 ccagttggaa aaaaagtacg gagatctcat caaagacact ttgggcaaaa acggtgtaag
     1441 cgttaagcaa atagattata agatggccgc ggcatcggca ctaccgaggc aaactcccga
     1501 agccgcgccc gaaatattaa accctcaacc ggccgtgact tcctcggcaa cagctagtcg
     1561 tcagcaggcc gttagcaaac ccgccggtaa tagcctggtt cattcctacc ggcagggctt
     1621 gaacgagcgc tatacattcg ataattttat cgtcggcgcc agtaacgagc tggctttcgc
     1681 cgcttgccaa gccgtttcac agcagccggg aactcgctat aacccgctct tcttgtatgg
     1741 tggcgttggc attggcaaaa cccatctgat gcaagcggtt ggcaatatat tagctattcg
     1801 caatcctgac gcacgggtgc tgtacatcac taccgagcaa tttgtgcagg agtttgtgga
     1861 tgccttgcgc tttagaaaga ccgccgattt tacccgtcac tatcgtaccg ccgatatttt
     1921 aattgttgat gatttccagt tcatcgccgg caaagagaag gtgcaagagg agtttttcca
     1981 taccttcaat gcgttgcatc aggccaacaa gcagatcatt atcagcgccg ataagccacc
     2041 taaagacatt ccgacgcttg aggaccgctt gcgctcccgc ttcaatggcg gcatgagcat
     2101 cgacatgcag attccagact ttgaaacccg ctgtgctatc gtcaaaaaca aggcccaaaa
     2161 ccacgacacc gaactcccga ttgacgttgt cgaatattta gcggcaaatg ttcagaccaa
     2221 tatccgcgag ttggaggggg cactgaatca gcttttggcc ttttgcgaga tgcgagcctt
     2281 ggagccttcg ctccccatcg tttccagcct tctcagcgtc agtaaggctc ggcccaaaca
     2341 cctgagcgcg aagcaaatca ttgagcgtac ggcgcgccac ttccaaatac cactcgaaga
     2401 catcctcggg ccaaggcgcg ataaagatat agtggtgccg cgacaaatcg ccatgtatat
     2461 gctgaggagt gagctacatc tgagctttcc taaaatcgcc cgtgaactgg gccgcaaaga
     2521 ccacacgact gctattcatt ccgtcgataa aatcgagaaa gaagctcggg ttgactcctc
     2581 gatcaggatg gccatcgccg acatcaagga gcatttatat gcttaaaaac tcattgacag
     2641 cctgtgcaca cattgtgggt aaagtgtttg ttaattgtgt acgatcaatg gcttttcgca
     2701 caaggcaaga tgcttatgct gcgggctatg tgcaaaaacc cgctctttta tccgcctttt
     2761 atccgccgct tttacctcgt cttatccacc gcaaaaatct tgcttactca tctgttactg
     2821 tccaggtttt acccaccatc cacaggccct ataaatataa ttacgaatta ataaactaat
     2881 tactacatag taggagcgtc cgaacatgaa gctacaagtc acccaggaaa acctaaaccg
     2941 agcgcttagc tcagttgccc gtgttgccaa tactcgcggt accttgccga tcctcgcgaa
     3001 cgtattgatc aagactagca ataaccggct cagtatctca gctaccaatc tcgacatcgc
     3061 catcacccac tacattggcg ctaaggtaag tgaagagggt tcgattaccg tccctgcccg
     3121 tctcatgcag gattttgtcg gcagcttacc cgaaggagtt attgagcttg acctcaaaga
     3181 caccaaactc cacgttacca ccgagcaata caactccgta gtgaacggca tagtggccga
     3241 cgactttccc gtcatgccaa cgatcagcaa aggcagcaaa tggacggtgg atggcgctat
     3301 atttaagcgt ggcttacagc aggttgtttt cgcagccagc agtgatgaga cccgccccgt
     3361 tttaacagga gtactcctgc aaaccatgga cggcaaattg ttcatggctg ctaccgacag
     3421 ctatcgcctc accgaaaagc aactgggcgc caataaagaa actgtgcagc tgctcatccc
     3481 cgcctcagct atgcaagatc tgctgagagt gctcggcgac ggtgaagaaa aagtgcaggt
     3541 gacccacgac gatcaacaag tcctgtttca agtcggagat gctgagttgg ttactcgcct
     3601 ggtcgaagga aactacccag actaccgcaa actaattccc gaaaaattcg cgacccaagc
     3661 cacgtgcaag cgcgccgact ttttgaatgt cactaaagtt tccagcttgt ttgcccgcga
     3721 gagcgccggc agtgtgacca ttgagattga tgagaagtct agccagctca gtatccgctc
     3781 ggtcgcttcc cagctcggtg aaaataccgc caccgccaaa gctaaagtca ccggcagtgg
     3841 tagcataacc ctaaattcac gctacttact cgatgccttg ggcgccttgc atggcgagga
     3901 ggtggtcttt ggttttaatg ggaagttgga gcccaccctc ctacatgacc cgaccagtgc
     3961 agattaccgg cacgtcatca tgccacttaa gagttaagta ctagtagaat taaggcatgc
     4021 tcgccgattt gcacctgcaa cacttccgct cctacaaaga tgattcgttt gagttttcgc
     4081 ccggagttaa tatcgttgtc gggccgaatg ccagtggtaa aaccaatttg ttggaagcgg
     4141 tgctggtcct ggcgcgcggc aactcttatc gggctaaaga tggcgagctg gtacaatttg
     4201 atcactcctg ggcacggctc gacacgcacc tcttaagcgg ctcggagcga acaattaaac
     4261 tctctcccga agccactccc gcaaaactgt acgagctcga cggcaaaagc tacaagcggc
     4321 tgaccctaaa ccatacctta ccggtagtgc tatttgagcc aaaccatctg cagctactct
     4381 caggtagccc tgaccgtcgc cgtgattacc tggatgattt gctcgagcag acagtagtcg
     4441 gctacggcaa tctgcggcgg caatacaaac gtgctctggc ccagcgcaac gccttactta
     4501 agcagcctgg gatcagtacg cagctctttc cttggaatgt gcgcctcagt gagctggctg
     4561 gccagatagt caagcaacga agccaattag tgacgcagct ggcaaaggcc gtcacagacc
     4621 tttacaagga tttatcacac actaaaacca gggtcagcct acagtacgaa tgcccctggc
     4681 cccaaggcag ctacgaaagc cagctcctca agaaactgga aatgaatgcc gaactcgata
     4741 gtcagcgcgg cttcacgagc gccggacctc accgcgaaga tctgagtgtg ctatttgacg
     4801 gccgcccctc acaggaaacc gcttcccgcg gcgaagtccg taccgccgta ttggctttga
     4861 aaatcaccga gctcaagc
//
LOCUS       DATNGP010000104         5877 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_1982568, whole genome shotgun sequence.
ACCESSION   DATNGP010000104 DATNGP010000000
VERSION     DATNGP010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5877
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1982568"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            82..1059
                     /locus_tag="VN778_02555"
     CDS             82..1059
                     /locus_tag="VN778_02555"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: protein motif:HMM:NF006829.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase 3"
                     /protein_id="HXR49884.1"
                     /translation="MANHRPVLGVEVTGWGMALPRSSYTNDELIAQYGLDSDNEWIRT
                     RTGIESRYIAEADETVVTLGAKAGRLALESAGLAPPAAIDYLFLGSTTSPTYSTVPGS
                     HPAIVKQLGEQGYDIQASEETNTACTSFVTALLNSYRQFAVDGIERALVIGADKLSAI
                     TEYRDRSTSILFADGAGAVVMEKTERRSGLLGWSQETKGSLDTLLSSPPGGTLQMEGP
                     EVFKHAVSFMVEHARNALQEAETDTDEIALVVPHQANVRIIEAANRRLGIDMDKTAVS
                     IGHFGNTSSASIPLALTEALHAGRLVRGEKALLVGFGAGMTVAAAVIEW"
     gene            complement(1073..1810)
                     /locus_tag="VN778_02560"
     CDS             complement(1073..1810)
                     /locus_tag="VN778_02560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49885.1"
                     /translation="MKVRKSTVLNCFSPPVMLATLTIESVLAIYTVWRYKMSTVTRLI
                     TGSLVMLGSFQLAEYFVCTGYGLHAEQWSRLGFVAITTLPPLGLHILHELADKPSRRL
                     VPAAYATMAGFIVFFLAYHSAFIGHQCTGNYVIFQLGERVGGAYTIYYFGWLLTSLVL
                     GWRWANQFMEKGKVASTKLETVRALMVGYLVFLVPTALSLTFQPSTRRGIPSIMCGFA
                     VLFALILSFYVLPRAAEQKAPALTKQG"
     gene            complement(1856..2260)
                     /locus_tag="VN778_02565"
     CDS             complement(1856..2260)
                     /locus_tag="VN778_02565"
                     /inference="COORDINATES: protein motif:HMM:NF020157.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1761 domain-containing protein"
                     /protein_id="HXR49886.1"
                     /translation="MNWDINVVAGLVAGVAAMAPGAIIYSPSVLGNVWMKEIKHKPGE
                     GGSPSAAVAKMFITSLINGLMASLVVWTAGAHTFSEALRLTMVSVGWFWVSGSLMLVF
                     FENRSWKWFQISALSHVTTAAVIGVVLGLFVK"
     gene            complement(2332..2988)
                     /locus_tag="VN778_02570"
     CDS             complement(2332..2988)
                     /locus_tag="VN778_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020404739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HXR49887.1"
                     /translation="MKQADCIQKTADFVKAKFSGEGTGHDWWHMYRVWQLAKTIVAEE
                     KEADLFVVELGALLHDIADWKFHNGDEEAGPKAAREWLESIGVQEAVISQIEDVIRTV
                     SFKGAGVASAMKTIEGKIIHDADKLDAIGAVGIARTFAYGGAHNREIYNPTISPERHD
                     SFEAYKNSKGPTINHFYEKLLLLKDRMFTETAKRIAEQRHVYMEAYLKEFFAEWEGDR
                     "
     gene            complement(2985..5003)
                     /gene="ligA"
                     /locus_tag="VN778_02575"
     CDS             complement(2985..5003)
                     /gene="ligA"
                     /locus_tag="VN778_02575"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008578413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HXR49888.1"
                     /translation="MNKPQAAERIARLRELINDYRYHYHVLNESIMSEEAADSLKHEL
                     TQLETEFPDLITPDSPSQRVAGTPRPGFQQVEHSTRMLSLNDVFNEAEVQAWEERIKK
                     LLPESAKLEYFADIKMDGLACALVYEEGVLVRGITRGDGYVGEDVTANVRTIDSVPLR
                     LRADKQHEHLLRGRTEVRGEIVMYKQDFAKLNEERAKAGKPLFANPRNTAAGTIRQLD
                     PKLVVSRPLHFRGYDLLRVNAEDVPTHAYAYEAMGALGFLVSREAKVLKSVEAIMGFA
                     NAWEARRQELAYNTDGLVIKVNDRRLYQRLGVVGKAPRGAIAFKYAAEQATTKVKDIF
                     VSIGRTGKATPVAMLEPVVVAGSMVQMATLHNESEVHRKDIRIGDTVILHKAGDIIPE
                     IVEPLVKLRDGSEKVFQMPIHCPECNTKLVKSKADDADWRCPNEACPSRSWKRIEHFA
                     SKPALDIEGLGEKNVIALINAELIKSTPDIYTLQPEDIMKLDRFAEISANKLVNAIQA
                     KKTPLLSRFIYGLGIRHIGVQTAVDLANHFRTVEALSEATIDDLAEVEGIGEVVAESL
                     VEWFSEPRNQRMLEKFKKHGVWPEPVKRVGGKLSGQNFVVTGSLESMSREQAAERIRA
                     LGGTFQSSVGKDTDYIVVGANVGASKLTKAAKLGTKQINEEEFLDILK"
     gene            complement(4996..>5877)
                     /locus_tag="VN778_02580"
     CDS             complement(4996..>5877)
                     /locus_tag="VN778_02580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49889.1"
                     /translation="AKNAPAPQLATEPVRLPNAWRLTKKTAQLLWQHKGLFIGITLVY
                     GVLNLILVQGLATGTDVSSLKHTLNQVFTGHLGSLASGLTVFVVLIGSAGNGSSQTAG
                     AYQLFLALITSLAVIWALRQLLTSARLRIREAYYRGMYPLIPFILVLLVIGLQLVPLL
                     IGAGLYSTVITNGIAVHVAEKVLWAIIFALLAALTFYLISSSLFALYIVTLPDMTPMK
                     ALRSARDLVRHRRLSVLRKILCLPVVLFVIAAIIMVPIIIVLTPLAQWIFFVLTMFSL
                     VAVHAYMYTLYRELLNE"
BASE COUNT         1461 a         1565 c         1461 g         1390 t
ORIGIN      
        1 tattaccgtt tatgcttact tgctccagta tacaacaata cccatctaaa acagctcgct
       61 gctatataat aatgagcaat catggcaaat catagaccgg tactaggagt tgaagttacg
      121 ggctggggga tggcacttcc ccgcagtagc tataccaacg atgaactgat agcgcagtat
      181 gggctcgatt cggataatga atggataaga acccggacgg gcattgagtc gcgttatatt
      241 gcggaggcag acgagactgt cgtcactttg ggtgctaaag ccggcagact ggctctggag
      301 tcggccggct tggcaccgcc ggctgctatc gattatctct tcttgggcag cactacttcg
      361 ccaacctact ccacggtgcc aggctcgcat cccgctatcg tcaagcagct aggcgaacag
      421 ggttacgata tacaggctag cgaagaaacc aatactgcct gtaccagctt tgtgaccgcc
      481 ttattgaatt cgtatcgcca atttgctgta gatgggatcg agcgggcttt agttataggg
      541 gccgataagc tgtcggccat tacggaatat cgtgatcgaa gcacgagtat attgtttgcc
      601 gatggggctg gagcggtagt catggaaaag accgagcgcc gcagtggctt gctcggctgg
      661 tcccaggaaa cgaaaggctc gctggacaca ctactttctt ccccgcccgg aggaactctg
      721 caaatggaag ggcctgaagt tttcaaacat gctgtgagtt ttatggttga gcatgcccgt
      781 aatgcgcttc aagaggccga aaccgacacc gatgaaattg ccctagtagt gcctcatcag
      841 gctaacgtac gaattatcga agccgccaac cgtaggctcg gcatagacat ggataaaacc
      901 gcagtatcga taggccattt tggtaacact tcctccgctt cgataccttt ggcattaact
      961 gaagccttgc atgccggccg cttagttcgc ggagaaaaag ccctgttagt tggttttgga
     1021 gcaggcatga ctgtagctgc cgcagttatt gagtggtagc aagcttgtgg cgctaacctt
     1081 gctttgtgag agcgggcgct ttttgctcgg ccgcccgtgg caaaacatag aacgataaga
     1141 tgagcgcgaa tagcacggca aagccacaca tgatactggg gatgccgcga cgggttgacg
     1201 gctggaatgt cagtgacagc gctgtcggta ccaaaaaaac aagatacccg accatgaggg
     1261 cgcgcacggt ttccagcttg gtgctggcca ccttaccctt ttccatgaac tgatttgccc
     1321 agcgccagcc taatactagg cttgttaaga gccagccgaa ataatagatg gtgtaggcac
     1381 cgccgacgcg ttcgccgagc tgaaaaatca cgtaattacc ggtgcattgg tgaccgataa
     1441 atgccgagtg ataagccaag aaaaatacaa taaaaccggc catggtggca taagctgccg
     1501 gcaccagccg ccggctaggt ttatcggcca gctcgtggag aatgtgcaag ccaagcggcg
     1561 gcaaagtcgt aattgctaca aaccccaggc gcgaccactg ctccgcatgc aagccgtagc
     1621 ccgtacaaac aaaatattcc gccagctgaa aacttccgag cataaccagc gagccagtga
     1681 taagccgcgt cactgtgctc attttgtagc gccacaccgt gtagattgcg agcaccgatt
     1741 caatagttag cgtcgccagc atcacgggcg gcgaaaagca attcaaaact gtcgatttcc
     1801 gcaccttcat agccacgagt atagcacagc ataaccggta tttagcctgt aaaaattact
     1861 tcacaaagag gcccagcaca acgccgataa cagcagccgt agtgacatga ctgagcgcac
     1921 taatctggaa ccacttccag ctacggtttt cgaagaagac caacatcaag ctaccggaca
     1981 cccagaacca ccctactgat accatcgtga gtctgagcgc ctcgctaaac gtgtgagcac
     2041 ctgccgtcca gacaacaagc gaggccatta agccattaat gagcgaagta ataaacatct
     2101 tagccaccgc cgcactcgga cttccgcctt cacccggttt atgcttgatc tctttcatcc
     2161 acacattacc gagcacactc ggcgaataaa taatcgcgcc cggcgccata gccgccacgc
     2221 ctgcgaccaa ccccgccact acattgatat cccagttcat cccaaaccct ccattaatgt
     2281 aattcgtact atatgctcgc cggctaaaat tgcaagcatt tcaatgacag gctagcgatc
     2341 accttcccac tcggcaaaaa actccttgag ataagcttcc atgtatacat ggcgctgttc
     2401 agcgatccgc ttggccgttt ctgtaaacat acggtccttg agtagaagca gtttttcata
     2461 aaaatgatta atggtcggcc ctttgctgtt tttgtaagct tcaaaagaat catgccgttc
     2521 aggtgaaatg gttgggttat agatttctcg gttatgcgca cctccataag caaaagtccg
     2581 ggcaataccg acagcgccaa tagcatcaag cttatcggca tcatggataa tcttaccctc
     2641 aattgttttc atggcggaag caacgccagc acctttgaac gagaccgtcc ggataacgtc
     2701 ttctatctgg ctaattactg cttcttgcac gccgatgctc tccagccatt ctctggccgc
     2761 tttagggccg gcctcttcat cgccattgtg gaatttccaa tctgcgatgt catgaagcaa
     2821 cgcccctagc tcgacaacaa acaggtctgc ttccttttct tctgccacaa tagtcttcgc
     2881 cagctgccac acgcggtaca tatgccacca atcatgtcca gtgccttcgc cactgaattt
     2941 agcttttaca aaatctgccg tcttttgaat acagtcagct tgtttcattt gagaatgtcc
     3001 agaaactctt cttcgttgat ttgcttggtg ccgagcttag cggctttggt taatttgctg
     3061 gcgccgacgt tggcgccgac cacgatgtag tcggtatctt tgccgacgga gctttggaac
     3121 gtgccgccga gagcgcggat acgttcggcg gcttgctcgc ggctcatgct ttctagcgag
     3181 cccgtgacaa cgaagttctg cccgctaagc ttgccaccca cccgcttaac cggctcgggc
     3241 cagacgccgt gctttttaaa cttctcgagc atacgctgat tgcgcggctc gctgaaccac
     3301 tccacaagtg attcggcgac cacttcccca atcccctcta cttccgccag gtcatcgatt
     3361 gtggcttcag ataaggcttc tacggtacga aaatgattgg ccaagtcgac agcagtctgc
     3421 acacctatat gtcggatgcc caaaccgtag atgaatcggc taagcagcgg tgtttttttg
     3481 gcctgaatgg cattaactaa cttattggca gaaatctcgg caaagcgatc gagtttcatg
     3541 atatcttcgg gctgcaaggt atagatgtca ggggtgcttt taatcagttc ggcgttgatc
     3601 aaagctatga cgtttttctc cccgagacct tctatgtcta gggccggctt gcttgcaaag
     3661 tgctcgattc gcttccagga acgagaagga caagcttcgt ttggacaacg ccaatcagca
     3721 tcatcagctt tactcttaac aagtttagta ttgcattctg ggcagtggat aggcatttga
     3781 aatacttttt cagaaccatc tcgaagtttt acaagcggct ccactatttc tgggataatg
     3841 tcccctgctt tgtgaagaat aaccgtatcg ccaatgcgta tgtcttttcg atggacttca
     3901 ctttcgttat gcaaagtagc catctgcacc atgctgccag ccactacgac cggctccaac
     3961 atcgccacgg gggtggcttt gcccgtccgg ccaatcgaaa cgaaaatgtc tttcacttta
     4021 gtggtagctt gctccgccgc atacttgaaa gctatggctc cgcgtggcgc tttaccaact
     4081 acgccaagcc gttgatataa tcggcggtca ttgaccttaa taaccaagcc atcggtgtta
     4141 tacgccagct cctgacgcct agcttcccag gcatttgcaa aacccataat agcctcgacg
     4201 cttttcagta cttttgcttc tcggctcacc aaaaagccca gagcccccat cgcttcataa
     4261 gcataggcgt gagtgggaac gtcttccgca ttgacgcgca ggagatcgta gcccctgaag
     4321 tggagcggcc ggctaaccac gagttttgga tcaagctgcc ggatggtgcc tgccgcggta
     4381 ttacgaggat ttgcgaaaag cggcttgccg gctttagcgc gttcttcatt aagtttggcg
     4441 aaatcttgct tgtacatcac gatctcgccg cgaacttccg tacggccacg cagtaaatgc
     4501 tcatgctgct tatcggcgcg cagtctaagc ggcactgagt cgatagtgcg gacattcgcg
     4561 gttacatcct ccccgacgta gccatcgccg cgggtaatcc cgcgcaccag gaccccctct
     4621 tcatacacca gcgcgcaggc cagaccgtcc attttgatgt cggcaaagta ttccagcttg
     4681 gcgctttccg gcagcaactt tttaatacgc tcttcccaag cttgcacttc ggcttcatta
     4741 aagacgtcgt tcaggctaag catacgggtg ctatgctcca cctgctgaaa accaggtcgc
     4801 ggcgtcccgg ccactcgttg cgaaggcgag tcaggcgtaa tcaggtcggg aaattcggtc
     4861 tcgagctgcg tcaattcatg ctttaagcta tccgcggctt cctcgctcat aattgactcg
     4921 ttaagcacgt ggtagtgata acgataatca ttgatgagtt cgcgtaagcg ggcgattcgt
     4981 tctgcggctt gtggcttatt catttagtag ctcccggtaa agtgtgtaca tataggcatg
     5041 aacggccacc agcgaaaaca ttgtcaacac gaaaaatatc cattgcgcca gtggcgttaa
     5101 gacaataatg atcggtacca tgataatagc cgcgatgaca aacaagacaa ctggtaagca
     5161 gagaatttta cgcagcacag ataggcggcg atggcggaca aggtctcggg cagaacgtag
     5221 ggctttcata ggtgtcatat caggtaaggt tacgatgtag agcgcgaaca gcgacgagct
     5281 aatcaagtaa aaagtcagtg ccgctagtag cgcaaaaata attgcccata aaactttctc
     5341 agcaacatga acggcgatgc cattcgtgat aactgtgctg tacagccctg caccaataag
     5401 cagcggtacg agctgcagac caataaccag taagactaaa ataaagggta taagcggata
     5461 catcccacgg taataggcct cgcggatgcg gagccgcgca ctcgtcagaa gctgccggag
     5521 cgcccaaata acagccaaac tggtgatcag tgccagaaag agctggtaag caccggctgt
     5581 ttggctggag ccattgccag ctgagccgat gaggaccacg aacacggtta gtccggaagc
     5641 cagtgagcca agatgaccgg taaaaacctg attaagcgtg tgtttgaggc tgctcacatc
     5701 cgtgccagtt gctagccctt gcactaaaat aagattgagt acaccgtaaa ctaaggtgat
     5761 accaataaag agacctttgt gttgccagag tagctgggcg gtctttttgg tgagccgcca
     5821 ggcgtttggc aggcggaccg gctcagtggc aagttgcggg gccggagcgt ttttagc
//
LOCUS       DATNGP010000105         2446 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_3154682, whole genome shotgun sequence.
ACCESSION   DATNGP010000105 DATNGP010000000
VERSION     DATNGP010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2446
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3154682"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            115..1569
                     /locus_tag="VN778_02585"
     CDS             115..1569
                     /locus_tag="VN778_02585"
                     /inference="COORDINATES: protein motif:HMM:NF015714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HXR49890.1"
                     /translation="MTRRRLHASWLVAWLSISITGGIAFAAIAKTAWAAAVPVLATSV
                     ILMAVAFLVRTRWTVLLIIVTGLSIGFWRGGIEQTGLQRYNLFYGRSVIVQGVVSDDI
                     STGPGGDERLTLKDLAINGVRLNGYLWISTDSTVRIQRSDRVTLKGKLGLGFGSIAAS
                     MFHATITHVARAQPGDIGLHVRDWFAGGIRAAIPEPEASLASGYLIGQRSALPQDVDE
                     QLKTVSLTHAVVASGYNLTILVSLARQLMVGVSKYLAAFSAVTMMGSFILISGASPSM
                     TRAGLVTSLTLAAWCYGRKIHPIVLLPFVAAITALFNPFYVWGDIGWYLSFTSFAGVI
                     ILAPLVQHALWRNKEKPGLVMQTVIDTMSAQLATLPIMLLAFGHYSPYALLANLLVLP
                     LVPLTMLLSFIAGVIGLSLPAAAHFVGLPADFILRYMLWAVSHIAELPGAQGELTITV
                     PAAFACYVLLGFGVCVLGQRLHFSFRSEPEINSA"
     gene            1594..>2446
                     /locus_tag="VN778_02590"
     CDS             1594..>2446
                     /locus_tag="VN778_02590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49891.1"
                     /translation="MNNQLNSLTRITRQFHNALITRRVDMNKLRRFGKLLYLWFSALV
                     MIVLLTVQSASAVTATTITVGETPMPSTAPDLIVTSIVGNARPMYVELYNQGDSPINL
                     AGWQVNLIIHDGVLSGCADVHATIDLPNAWLLSKKYVTLEDTSLPQTNSLTVPFFIDS
                     AALTSCASPKLTTVYVINATANTEQVVTIPANEWSVTTLLVAQHRQRSNSPSSARSIT
                     NIFDTDYKFVTGAVALNSDPLYLPPADSAGLQISEILPNARSCSPTESDPSCNDYIKL
                     YNPSQRPI"
BASE COUNT          544 a          681 c          602 g          619 t
ORIGIN      
        1 catgcagcgc attttagata cctgccccac gcaggtacta cccctcgcag tataaagctg
       61 agttttagat taattttcct tttaccatcc tgtgcacatg ccataatgcg attcatgacg
      121 aggcggagat tgcatgcatc atggctcgtt gcatggcttt cgatcagcat tacgggcggc
      181 atcgcatttg ctgcaatcgc taaaacagca tgggcggctg ccgtgcctgt cctagccact
      241 tcagtcatac ttatggctgt agcatttttg gtacgcactc gctggacagt tctactcatc
      301 attgtcaccg ggttatcaat tggtttctgg cgcggcggaa tcgagcaaac tgggctgcag
      361 cggtacaatt tattttacgg tagatctgtc atcgtccagg gcgtagtgag cgatgatatc
      421 agtaccggcc caggcggcga cgaacgcttg acgctaaaag acctcgccat taatggcgtt
      481 cgcctaaatg gctacttatg gatcagtaca gacagtaccg tgcggatcca gcgcagcgac
      541 cgcgtcactt taaagggcaa acttggcctg ggatttggca gcattgccgc cagtatgttc
      601 cacgcaacta ttacgcatgt agcacgagca caacccggtg atatcggtct gcatgttcgc
      661 gattggtttg ccggaggtat ccgagccgca attcctgaac cagaggccag cctcgccagc
      721 ggctatttga ttggccagcg tagcgccttg ccgcaagacg tcgacgaaca actgaagacc
      781 gtaagcctga cgcatgccgt cgtcgccagc ggctacaact taactatttt ggttagcctc
      841 gcacggcagt tgatggtcgg ggtctcgaag tatttggcag cattttcggc ggttactatg
      901 atgggcagtt ttatcctgat cagcggcgcc agcccgtcta tgactcgtgc cggtttagtt
      961 acgagcttga cactggcagc gtggtgctac ggccgcaaaa ttcacccgat tgttttattg
     1021 ccttttgtgg ctgctattac cgcactcttc aatccgtttt atgtatgggg cgacattggc
     1081 tggtatttat cattcacgtc gtttgccggc gtcattattc tggcaccgct tgttcagcac
     1141 gcgctgtggc gcaacaagga aaagcctgga ctcgttatgc aaactgtcat tgacactatg
     1201 tcggctcagc tcgccacgct gccgattatg ctgcttgcat tcgggcatta ttcgccatat
     1261 gcactgctcg ctaacctgct agtactaccg ctcgtgccgc tgacaatgct gctgtcattt
     1321 attgctggag ttatcggtct gtcactgccg gcagccgccc atttcgtggg actgccggct
     1381 gattttattt tgcggtacat gctgtgggct gtatcacata ttgcagaact cccgggtgca
     1441 caaggtgagc taacaataac ggtaccggca gcgttcgctt gctacgttct gcttggtttt
     1501 ggcgtatgcg tactaggaca gcgtttgcac tttagctttc gcagcgaacc tgaaatcaat
     1561 agcgcctaga tgccgggaca aggttacaaa tacatgaaca atcagcttaa ttcgttgaca
     1621 cgaatcactc gtcaattcca caatgcactc atcaccagga gggttgacat gaataagcta
     1681 agacgttttg gtaagttatt gtacttgtgg ttttccgctc tcgttatgat cgttctgctg
     1741 acggtccagt ctgccagcgc agtgacggct acgaccataa ctgtcggcga gacgccgatg
     1801 ccaagcaccg cgccagacct catcgttacc tcgattgtcg gtaacgctcg gccgatgtat
     1861 gtcgagctct acaaccaagg cgactcgcca atcaacctag ctggttggca ggtaaacctt
     1921 atcattcatg acggcgtcct gagcggctgt gccgatgtcc acgccaccat cgacctgcca
     1981 aatgcttggc tcctttccaa aaaatacgta acactagaag atacatccct cccccaaact
     2041 aacagcttga cggtgccatt ttttatagat tcggcggcgc tcactagctg cgcgtcgccc
     2101 aagcttacaa ccgtttatgt tattaacgct acggcaaaca ctgaacaggt agtcactatc
     2161 ccagcgaacg agtggtcggt cacgacactc cttgtcgccc aacaccggca gcgcagcaat
     2221 tcgcccagct cggcacgcag tatcacaaac attttcgata ccgactacaa gttcgtaacc
     2281 ggcgcggtag cactcaatag tgacccgctg tatctcccgc cggccgatag tgccggtctg
     2341 cagatatcag aaatcctgcc aaacgcccgc tcatgctccc caaccgagtc cgaccctagc
     2401 tgcaatgact acataaagct ctacaatccg tcgcaacggc caatta
//
LOCUS       DATNGP010000106         2652 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_2736757, whole genome shotgun sequence.
ACCESSION   DATNGP010000106 DATNGP010000000
VERSION     DATNGP010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2652
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2736757"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..828
                     /locus_tag="VN778_02595"
     CDS             <1..828
                     /locus_tag="VN778_02595"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HXR49892.1"
                     /translation="VIRPTSGLNMAVASSQLQTELQRITSQPISVNLGTQTVPVSAAT
                     IKSWVSITPDNSTAMDYLHVNNGVIVSSLTKLVNQYVKAPVNQVTVTHPDGTSAVIVA
                     GHNGTKLSDTANLSGQAQQIAKNLFSGNGFQVSAPLVTLPFQSVTPAAFDKLIEVNLS
                     AKQMWVYDHGNLLKQYAVSAGKPSTPTPTGEFHVYEKLPVQDMWGYNSDGTKYFQPHV
                     YWISYFTGGDAVHGVYWHPLSWFGVHNSSHGCVGLPDDEAQWVYDWDSIGTTVITYN"
     gene            complement(825..1388)
                     /gene="frr"
                     /locus_tag="VN778_02600"
     CDS             complement(825..1388)
                     /gene="frr"
                     /locus_tag="VN778_02600"
                     /inference="COORDINATES: protein motif:HMM:TIGR00496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HXR49893.1"
                     /translation="MNPNEVVTSAQTKLSQAVEHFKDNLKTLRTGRANAAMLDGVMVE
                     AYGTQMPLNQTATISVPEPQLIQITPFDPNNLAAISAAIRNNQQLGLNPADDGRVVRV
                     PIPPLNEERRRDLAKQVGQKQEEAMISLRNIRHEALDSVDKAKKDKSIGEDDAKRLTG
                     QIEEAMNKARAEIEAAAKAKETEILTV"
     gene            complement(1686..2282)
                     /locus_tag="VN778_02605"
     CDS             complement(1686..2282)
                     /locus_tag="VN778_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015254937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HXR49894.1"
                     /translation="MAIDIAEVKRLKDLTGVGLTDAKAALEEAKGDFDKALEAMRKKG
                     LTKAEKRGEREARAGVIGTYNHDGRIGVLVEVNCETDFVARNEIFQNLVKDLAMHIAA
                     ADPVYISENEIKESEFKAKRTEFSRLAEAEGKPRDMVSKIAEGQTKKYFAERCLLDQP
                     FVKNPDLTVGEYVKEHNARLGENIVVRRFSRLALGEVA"
     gene            complement(2282..>2652)
                     /gene="rpsB"
                     /locus_tag="VN778_02610"
     CDS             complement(2282..>2652)
                     /gene="rpsB"
                     /locus_tag="VN778_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013536851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HXR49895.1"
                     /translation="LENRMASGELANKYNKLEVQRFQEEIDQLNTIYGGIKELNKKPG
                     AVFIVDIVHDANAVREARKLGVPIVALVDTNADPSLVTYPIPSNDDAIKTIELMLGYV
                     QNAIETGKAKAKTTDKTEEK"
BASE COUNT          553 a          736 c          658 g          705 t
ORIGIN      
        1 gtcatccgac cgaccagcgg ccttaacatg gcagtcgcct ccagccagct acaaaccgaa
       61 ctgcaacgca tcacctctca gccaatcagc gtgaatctgg gaacgcaaac cgtgcccgtc
      121 agtgcggcca ctattaaaag ctgggtttcg atcacgcccg acaactccac cgctatggat
      181 tacctgcacg tcaataacgg cgttatcgtc agctcgctga ccaaactggt caaccaatac
      241 gtcaaagcgc cggtcaacca agtcaccgtt acgcatcccg atggtacgtc ggcagtcatc
      301 gtggccggcc ataacggcac caagctcagc gacaccgcta atctcagtgg ccaggcgcag
      361 caaattgcca aaaatctctt tagcggtaac ggttttcaag tgagtgcccc gctggttacc
      421 ctaccctttc agtcagtcac ccctgccgct ttcgacaagc tgattgaggt caacctgagc
      481 gctaagcaaa tgtgggtcta tgatcatggc aatctgctca agcagtacgc tgtcagtgcc
      541 ggcaagccat cgacgcctac cccgaccggt gaattccacg tctacgagaa attaccagtg
      601 caagacatgt ggggctacaa ctcggatggc acgaaatatt tccagccgca tgtgtactgg
      661 atcagctatt tcacgggcgg tgatgcagtg cacggcgtct actggcatcc gctcagctgg
      721 ttcggcgtcc acaatagctc gcacggctgt gtcggtttgc ctgatgacga agcgcagtgg
      781 gtctacgact gggactctat cggtaccaca gttataactt acaactaaac agtcaagatt
      841 tcagtttctt tggccttggc cgccgcctcg atttcagctc gcgccttgtt catagcctct
      901 tcgatctggc ccgttagacg cttggcatca tcctctccaa tggacttgtc ctttttggct
      961 ttatccaccg aatcgagtgc ctcgtgccgg atgtttctta gggagatcat ggcttcctct
     1021 tgcttttgcc cgacttgttt ggccagatca cgacggcgct cttcattgag aggtggaatc
     1081 ggcacgcgca cgacgcggcc gtcatcggcc gggtttagcc cgagttgctg attattgcga
     1141 atggctgcac tgatcgctgc caagttattc gggtcgaaag gagtgatctg gataagttgc
     1201 ggctcgggga cactgatcgt cgctgtttgg ttcagcggca tttgcgtgcc ataagcctcc
     1261 accataacac catcgagcat ggcggcattg gcccggccag tccggagtgt cttcaagtta
     1321 tctttaaagt gctcgaccgc ctggctgagc ttagtttgcg cactggtaac gacttcgttt
     1381 ggattcatga caggtcaatt atacctcatc cttatccgcc gcttgtaggg ctgctttgag
     1441 ctctaggtca taaattttca ttccattaac ctcaaccacg tcatcatcgt cctcatctga
     1501 tagttatact tcgtgaacca aaatctcgtg ccgcaaggtc atccacctgc tccactaatt
     1561 cattcggtag ccgaagagct actttagtat ttccctggtg agacatggtg gacaatgtac
     1621 gccatttgtc cgacattgtc caccagcttt taaaaatact gaaagaaggt aatgtccgat
     1681 ttagattagg cgacttcacc aagggcgagc cgcgagaaac gacggacgac gatgttttcg
     1741 ccgaggcggg cattgtgctc tttgacgtac tcgccaacgg ttaggtcagg atttttgaca
     1801 aagggctggt cgagcaagca gcgctcggca aagtatttct tggtctgccc ttcagctatt
     1861 tttgaaacca tgtcccgtgg cttaccttcg gcttctgcaa gcctagaaaa ttccgtccgt
     1921 ttcgccttga actcactctc tttaatctca ttttccgaga tgtatactgg atctgctgct
     1981 gctatatgca ttgcgagatc tttaactaga ttttggaaga tttcgttgcg ggccacaaag
     2041 tcggtttcgc agttcacctc caccaggacg ccgatacggc cgtcgtggtt gtaggtgcca
     2101 atgacgccgg cgcgagcttc gcgctcgccg cgcttctcag ctttggtcag gccttttttg
     2161 cgcatggctt cgagggcctt atcgaagtcg cctttggctt cttcgagagc cgctttggca
     2221 tcggtcagac cgacaccggt cagatccttc aaacgcttaa cttctgcgat atcaatggcc
     2281 attatttctc ctctgtctta tcggttgttt tggctttcgc tttgccggtt tcgatggcat
     2341 tttggacata gccgagcatt agttcaattg ttttgatggc gtcgtcgttg gacggaatcg
     2401 ggtaggtcac gagcgacggg tcagcattgg tatcaaccag cgccacgatc ggcacaccga
     2461 gcttcctggc ttcacgcacg gcgttggcat cgtgcacgat atcgacgata aagacagccc
     2521 ccggcttttt gtttagctct ttgataccgc cataaatggt gtttaactga tcgatttctt
     2581 cctggaagcg ctgcacttcc agcttgttat acttgttggc cagctcgccg ctcgccatgc
     2641 gattttctag at
//
LOCUS       DATNGP010000107         4926 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_3724040, whole genome shotgun sequence.
ACCESSION   DATNGP010000107 DATNGP010000000
VERSION     DATNGP010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4926
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3724040"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="VN778_02615"
     CDS             complement(<1..294)
                     /locus_tag="VN778_02615"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HXR49896.1"
                     /translation="MNSQAYRGPEVSAKLVICVDDKVLMLRRKNGQHIFPGGHIEWRE
                     PIMDCLKREAMEELSYKLIEESELFDIYEYISSDEPMHGLVLHYRLHLRKRPNL"
     gene            complement(448..846)
                     /gene="rpsI"
                     /locus_tag="VN778_02620"
     CDS             complement(448..846)
                     /gene="rpsI"
                     /locus_tag="VN778_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007390646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HXR49897.1"
                     /translation="MAKTDLFHALGRRKTSTARVRLTSGKGSLTVNDKPAAEYFADSK
                     YLLAKLNEPFAVLDLENKYDITVKVDGGGHPGQAEAIRLGIAKVLSAMNTDLKSTLKR
                     ADLLSRDPRDKERKKFGLKGARKARQFVKR"
     gene            complement(846..1289)
                     /gene="rplM"
                     /locus_tag="VN778_02625"
     CDS             complement(846..1289)
                     /gene="rplM"
                     /locus_tag="VN778_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019535621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HXR49898.1"
                     /translation="MQKTYSAKPSDITRKWYVIDASETTLGRVATKAASLLTGKGKPQ
                     FTQHIDCGDYVIVINAKDLKVTGNKLETIKYYRHSGYAGNLHTRTLKEQMNLDPTKAV
                     EHAIRGMLPVNKLRDARLERLKIYAGADHKHEAQKPETLSLKGAK"
     gene            complement(1409..1759)
                     /gene="rplQ"
                     /locus_tag="VN778_02630"
                     /pseudo
     CDS             complement(1409..1759)
                     /gene="rplQ"
                     /locus_tag="VN778_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015642143.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
     gene            complement(1762..2715)
                     /locus_tag="VN778_02635"
     CDS             complement(1762..2715)
                     /locus_tag="VN778_02635"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF003513.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HXR49899.1"
                     /translation="MSTMIHTPGIVLVDEHNATSHTFSIEPLHGGYGMTLGNSLRRVL
                     LSSIAGAGITSFKIEGVSHEFTTVPGVKEDVVDIMQRLKGIRFRVYGSDPQIIRIVKT
                     GKGVITAKDIQVNADVEVVNPDHIIATLDDPKAKFVMDLIVEVGRGYRQIEESTAKKP
                     SDHIALDTIFSPVLRVRYKVENTRVGQATDFDKLLLTIETDGTISPREAFEEAAAILV
                     NQYTALAGKTRLAAEQPVSEVAGDTDSTSALVSGDESSVLNTPIEELSLSARTANALI
                     NNDIHTIRDLFALNETELRELKGFGSKALDEVKEKLAELEL"
     gene            complement(2737..3357)
                     /gene="rpsD"
                     /locus_tag="VN778_02640"
     CDS             complement(2737..3357)
                     /gene="rpsD"
                     /locus_tag="VN778_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012475637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HXR49900.1"
                     /translation="MARDTQSIVKMSRREGYALHPKAHKALVKRPTIPGQHGQGGRMR
                     AKTSQYLLQLREKQKVKRLYGLLERQFSNLMTEASRTKGQSGAVLLQLLERRADNAIY
                     RAGFAPSRRAARQLMTHGHFMLNDRRVDIPSIRLKPGDKLTLREHSKGTEYFKKLDDI
                     SPAPTTTPGWVKVDRKKVSVEITGLPTRDDAEPDINEQLIVEYYSR"
     gene            complement(3359..3754)
                     /gene="rpsK"
                     /locus_tag="VN778_02645"
     CDS             complement(3359..3754)
                     /gene="rpsK"
                     /locus_tag="VN778_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003633055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HXR49901.1"
                     /translation="MADSTTNVKKKKAKRTVTSGQVHILATSNNTIITFTDPHGAALA
                     SSSAGACGFRGSKKSTAYAAQVATDKAATAAKQQYGFMKADVFVKGVGLGRDAAVRSL
                     AGHDIQVESITDVTGMPHGGVRPRKARRG"
     gene            complement(3767..4156)
                     /gene="rpsM"
                     /locus_tag="VN778_02650"
     CDS             complement(3767..4156)
                     /gene="rpsM"
                     /locus_tag="VN778_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015642147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HXR49902.1"
                     /translation="MAARISGVTIPNEKQIQVALRYLYGIGPSNALVILEEAKVEPTV
                     RAKNLTDAEVARIQDIINAKYTVEGELQRIVAGNIKRLKDIKSYRGLRHTANLPTRGQ
                     RTRTNARTRRGKRVTVGGTAKKAPSKT"
     gene            complement(4159..4275)
                     /gene="rpmJ"
                     /locus_tag="VN778_02655"
     CDS             complement(4159..4275)
                     /gene="rpmJ"
                     /locus_tag="VN778_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011160599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HXR49903.1"
                     /translation="MKVRASVKKISPDDKIVRRKGRVYIINKLKPKHKQRQG"
     gene            complement(4450..4671)
                     /gene="infA"
                     /locus_tag="VN778_02660"
     CDS             complement(4450..4671)
                     /gene="infA"
                     /locus_tag="VN778_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009148344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HXR49904.1"
                     /translation="MAGNKEVIELTGTIVETLPGTQFRVELENGHRIIAHVAGKMRKH
                     FIRIVPGDKVTVELTPYDLSKGRISRRLS"
     gene            complement(4723..>4926)
                     /locus_tag="VN778_02665"
     CDS             complement(4723..>4926)
                     /locus_tag="VN778_02665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49905.1"
                     /translation="ISIILKQNITTALTAVIDDGGFMDSSITMAEVRAINVHNRPERL
                     GDYLHGVLMLIFGSAEANENSSA"
BASE COUNT          997 a         1251 c         1340 g         1338 t
ORIGIN      
        1 taagtttgga cgtttgcgaa gatgcaatcg atagtgcagg actaagccat gcataggttc
       61 atccgaagag atatactcat agatatcgaa taactcggat tcctcgataa gtttgtagct
      121 gagttcctcc atagcttcgc gcttcaagca gtccataatt ggttcgcgcc actcaatatg
      181 gccaccagga aagatgtgct gtccgttctt acggcgtagc atgagtactt tgtcgtcaac
      241 gcaaattacc agctttgcgc tgacctccgg tccacggtac gcttgtgaat tcatctttgg
      301 gattatagcc tgaactcgtc tcaaagcaaa aaccccgatt tctcggggtt tctgtagttc
      361 tgctgtttcg cgttgcagtc caggggagag agcccgagca actgtattcc atttgcacgg
      421 cgcgaagaga ggggatgttc ccctctctta tcttttaaca aactgacgag ccttgcgagc
      481 gcccttcaag ccgaacttct tgcgctcttt gtcgcgcggg tcgcggctta agaggtcagc
      541 acgcttcagc gtactcttga gatcagtgtt catggcgctt aagactttgg cgatgccgag
      601 gcgaattgct tcagcttgac cagggtggcc gccaccgtca actttgaccg tgatgtcgta
      661 cttgttttcc aggtcaagta cggcaaatgg ttcattcagc ttagcgagga ggtacttgct
      721 gtcagcaaaa tactcagcag ccggcttgtc gttaaccgtg agcgaacctt taccgctggt
      781 cagacgaaca cgagcggtgg acgtcttgcg gcgtccaagc gcgtggaata gatcagtttt
      841 agccattatt tggctccttt caatgacagc gtctcgggct tctgagcctc atgcttatga
      901 tcagctccgg catagatttt caggcgctcc aaacgggcat cgcgaagttt atttaccggc
      961 agcatgccac gaatggcgtg ttcgacggcc ttggtcgggt ccagattcat ctgttccttc
     1021 agcgtacggg tgtgcagatt gccggcatag ccgctgtggc ggtagtactt gatggtctcg
     1081 agcttgttgc ccgtgacttt caaatctttg gcattgatga caatgacata gtcaccgcag
     1141 tcgatgtgtt gggtaaattg tggcttgcct ttgccggtca gcaggctggc tgctttggtc
     1201 gccacgcggc ccagcgtggt ttcggaagca tcaatgacat accacttgcg ggtaatgtcg
     1261 gatggcttgg cgctatatgt tttttgcatt atttggactc ctttttggcc actggcttgg
     1321 cagcaggctt cttggcagga gctttggttt cggcttttgg taccttagca gcgacagatt
     1381 tggccactgg tgcttctttg aggtcatcaa caaagctgac gcgcgccatt tgggtgttgt
     1441 caccgcgacg attgacagtc ttttcgatac gcaaatgacc actggtgcgg ccagatagct
     1501 ttggagcaat ctcatcgacc agcttgtggg cgcttgatag tgtctgcagg ctgctgataa
     1561 cctgacggcg attgtgcaaa tcacccttct tggccttggt aatgagcttc tcaacgtaag
     1621 gcacgatctc cttcgccttg ggaagcgtgg tttgaattga gccgtgcttc acgagtgcgt
     1681 cagccaagcc cttaatgagc gagcggcgag gaccgttttc gcggtgaaat ttacgacctt
     1741 gatatccgtg acgatgcatg tttagagctc caactccgcg agtttttctt taacttcgtc
     1801 gagcgccttg ctgccgaaac ccttcaactc acgtaactcg gtttcgttta gggcaaacag
     1861 gtcgcgaata gtgtggatgt cattattgat gagcgcatta gcggtgcggg cactcaggct
     1921 tagctcttcg attggggtgt tcagaactga cgattcgtcg cctgaaacaa gcgctgacgt
     1981 gctgtctgtg tcgccagcaa cttcgctaac aggctgttca gccgctaagc gagtcttgcc
     2041 agcaagcgcg gtatattggt taaccaatat ggcagcggct tcttcgaatg cttcacgtgg
     2101 gctgatggtg ccgtcggtct cgatagtcaa aagcagctta tcgaagtcag ttgcttgacc
     2161 aacgcgagtg ttttcgacct tgtagcggac gcgcagcact ggactaaaga tggtgtcgag
     2221 tgcaatgtga tcactcggtt tcttggctgt gctttcttcg atctggcggt aaccacgccc
     2281 gacttcgacg atcaggtcca taacaaattt ggctttcgga tcgtctagcg ttgcaataat
     2341 gtggtccgga ttcacgactt cgacatcggc attaacctgg atgtccttgg cagtgattac
     2401 gcccttgccc gtcttcacaa tgcgtatgat ctgcgggtcg ctgccatata cgcggaagcg
     2461 aatgcctttg aggcgttgca tgatatcgac gacatcttct ttaacgcctg gaacggtagt
     2521 gaactcgtgg ctgacgcctt caatcttgaa tgaggtaata cctgcaccag cgatgctcga
     2581 aagcagcacg cggcgaagcg aattaccaag cgtcatgccg taaccgccat gaagtggctc
     2641 aatgctgaag gtgtggctag tggcgttatg ttcgtcgacc agtacgatac cgggggtatg
     2701 aatcatagtt gacataagta cttctcctag cctcctttag cgtgagtagt attcgacgat
     2761 tagttgttcg ttgatgtccg gctcagcgtc gtcgcgcgtg ggcagacctg tgatttcaac
     2821 tgaaactttt ttgcggtcaa cctttaccca acctggtgta gtagttggag ccgggctgat
     2881 gtcgtcaagt ttcttgaaat attctgttcc tttgctgtgc tcacggagcg tcaatttatc
     2941 ccccggtttc agacggatgg atgggatgtc aacgcggcgg tcattcagca tgaagtggcc
     3001 gtgtgtcatg agctgtctag cggcccgacg tgacggcgca aaaccggcac gataaattgc
     3061 gttatcggcg cgccgttcga gcaattgcaa aagcactgct ccagattgac ccttggtgcg
     3121 gctagcctca gtcatgaggt tgctgaactg gcgttcaagc aatccgtaga ggcgcttgac
     3181 cttctgcttc tcgcggagct gaagcaagta ctgcgacgtc ttggcacgca ttctgccacc
     3241 ctgaccgtgc tggccaggga tggttgggcg cttgacgagc gctttgtgag ctttggggtg
     3301 aagcgcatag ccttcacgac ggctcatctt tacgatggat tgggtatcac gtgccatatt
     3361 agcctctcct tgcctttcgt ggacgaacgc caccatgcgg cattcctgtt acgtcggtaa
     3421 tggattcgac ttgaatgtcg tggccagcca gcgagcgaac ggcagcatcg cggcccaggc
     3481 ccacgccctt gacgaagaca tcagccttca taaaaccgta ctgctgctta gcggcggttg
     3541 cggccttgtc agtagcgacc tgagcggcat aggcggtgct cttcttggaa ccacggaagc
     3601 cgcaggctcc agcactggag ctggctagag cagcgccgtg gggatcggtg aatgtaatga
     3661 tggtattgtt cgaagttgcc agaatgtgaa cctgaccgct ggtcacggtt cgcttggctt
     3721 ttttcttctt aacattagtg gttgaatctg ccatatatta cccctcttag gtcttcgatg
     3781 gcgccttctt cgccgtacca cccactgtta cacgtttgcc gcggcgggtg cgcgcattag
     3841 tgcgcgttcg ctgaccgcgg gttggcaaat tcgcggtgtg gcgtaaacca cggtaggact
     3901 tgatatcctt caggcgcttg atgttgccag ctacgatgcg ttgcagctca ccttcgacgg
     3961 tgtacttggc attgatgatg tcctggatcc gcgccacttc ggcgtcagtc aggttcttgg
     4021 cgcgaacagt tggttcgacc ttggcttctt ccaagatcac cagcgcattg ctcgggccaa
     4081 taccgtacag atagcgcaaa gctacttgaa tctgcttttc gttcgggata gttacgccag
     4141 aaattcttgc agccatgact agccctgcct ttgcttatgc tttggcttta gtttgttgat
     4201 aatgtagacc cgccctttgc gacgaacaat tttgtcgtcg ggactgatct ttttgacgct
     4261 tgcacgcact ttcatcccgc ggttatctcc tttaggattt gaggcggact gggcttccct
     4321 gtgggaggcc gagctcactg cttagtttga tgttaattcg ctagcggaca atcgccgtag
     4381 tgtgcggccc taatcctggc ggtgctttcg cgcagcagga taacccgcgt actcttcggt
     4441 gagtgccctc tagctgaggc gacggctgat ccggccctta ctcaggtcgt aaggtgtcag
     4501 ctcaaccgtg actttgtcgc ccgggacaat gcgaatgaag tgcttccgca tcttaccagc
     4561 gacgtgggct ataatccgat gtccgttttc gagttcgacg cgaaattgag tacccggcag
     4621 ggtctcaaca attgttcccg tgagctcgat cacttcctta ttgccggcca tagatataac
     4681 tagaggtgaa ttatagcaga attaacaact gcctgtaaag tgttacgcgg aggaattttc
     4741 attggcttct gccgagccga atatcagcat caatacaccg tgcagatagt cgcctagacg
     4801 ctccggtcgg ttatgcacat ttatggcacg aacctctgcc atcgttatgc tgctatccat
     4861 gaatccacca tcatctatga ccgcagtcag tgcagttgta atgttctgct tcaaaattat
     4921 agatat
//
LOCUS       DATNGP010000108         9544 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_4460055, whole genome shotgun sequence.
ACCESSION   DATNGP010000108 DATNGP010000000
VERSION     DATNGP010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 9544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9544
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4460055"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..724)
                     /locus_tag="VN778_02670"
     CDS             complement(<1..724)
                     /locus_tag="VN778_02670"
                     /inference="COORDINATES: protein motif:HMM:NF014803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49906.1"
                     /translation="MTLQTIEPEQVLHLPQFLNYTTPFFFTSREILRKNYLTFTHLFD
                     KAEVYYALKANSDSKILTYLNEIGSGFEAASAYEIAMLLEVGVDPKKIVYGTSIKLAE
                     HIRLAHQAGVDRFAADSKEEVKKIAEHAPGAKVFIRTIVEDTGSVFMMSERFGTPPDT
                     VKDMLLYARKLGLKTYGISFYVGSQAARHDMWARAIHTVQPIIEALLKKGITLEVLNI
                     GGGFPVHYDNHEDAPALRDIVIS"
     gene            complement(793..1686)
                     /locus_tag="VN778_02675"
     CDS             complement(793..1686)
                     /locus_tag="VN778_02675"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HXR49907.1"
                     /translation="MHNQLTIAILAGLGGMIGWGFADFCAKKTTDAVGAIKSLVWAHA
                     FGTTLFIIIALGDAWLFGRHLQFPNSFGTWAGSAFFGALQMIVYWLAYEAFGKGQLAV
                     LNPIFASFPGLVAVAAVIFFGETLSAGLAIALVLLFGGILLINIDPTGTRRSKRFNSI
                     PGLKEIALATILAAAWTIGWDRFISGRQALPYALSMYAFMLLAAVALAKLMRSSLRGV
                     PRELWKFLVLIGLGETVAYLAISWGYSATSLTSIVALVSGAFSLPTLVLAYIFLKERL
                     SKLQWGAVGLTILGIVLVSLQ"
     gene            1752..2123
                     /locus_tag="VN778_02680"
     CDS             1752..2123
                     /locus_tag="VN778_02680"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXR49908.1"
                     /translation="MPKIAIIEDDKAISQMYRLKFETEGFTVQTAENGKLGLELCEQM
                     KPDMVLLDLMMPEMNGDEMLAKFRATDWGKGIQVIILTNMGEQEIPPEVKALGVSAVI
                     LKADMTPRQVADVVKKLLAPA"
     gene            2129..3454
                     /gene="murD"
                     /locus_tag="VN778_02685"
     CDS             2129..3454
                     /gene="murD"
                     /locus_tag="VN778_02685"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HXR49909.1"
                     /translation="MKIAILGYDVEGRASYEYFKAQGHELTICDQNPAIAIPEGVPAV
                     LGENYLDELDRFDLLVRTAGLTPSKILEKNPAVADKITTHINEFFRACPTKNIIGVTG
                     TKGKGTTSTLITKMLEADGKQVQLGGNIGVPPLTFLGELTAESWVVLELSSFQLIDIR
                     YSPLIAICLMVVPEHLNWHSGLNDYTAAKSNLFAHQTAEDKAIYFAENDLSKLIAGHG
                     PGQKIPYFAPPGAFVDNGAVSIDGQVICQVSELQLRGAHNWQNVCAAVTAAWQIVQDV
                     DAIRSVLTTFSGLEHRLQLVGEVAGVKYYDDSFGTTPETAVVAIEAFTEPKVLITGGF
                     GKSTDFEALIDTILKSDVKHVVCIGKTGSVIADRLEARKEERCVTYTVWSSYDALIMP
                     EIVEAARQQADPGDVVLLAPADASFDMFKDYKDRGAQFSQAVQALATNA"
     gene            complement(3417..4064)
                     /gene="murI"
                     /locus_tag="VN778_02690"
     CDS             complement(3417..4064)
                     /gene="murI"
                     /locus_tag="VN778_02690"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HXR49910.1"
                     /translation="MKIGVFDSGVGGLSVANAIRKALPEHEVILREDKEHVPYGIRPP
                     QEILGLVVPIFQELVDAGCQVIVVACNTVSTTLIEELRARFAVPLIGIEPMVKPAAQL
                     TKSGVIAVCATPTTLLSPRYEWLKTTYAQDVVVLEPDCSDWAYMIEQKQIDKQKIADR
                     INEVLNQKADVIVLGCTHYHWIEEEIASLVAGQARVLQPEQAVIKRLWRELVRLG"
     gene            complement(4061..4867)
                     /locus_tag="VN778_02695"
     CDS             complement(4061..4867)
                     /locus_tag="VN778_02695"
                     /inference="COORDINATES: protein motif:HMM:NF014881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HXR49911.1"
                     /translation="MKLLNGSELAGFIKERQAHQVRALRQAHGVQPKLAIVVTIDNPV
                     IDLYIRLKQQYGADILIDVDVHRVKQTETEELLNKLSIDKSVHGVIVQLPLEKPEQTD
                     KIVNLVAPEKDVDALGERALFEPATPMAILWLLSGYNVDLAGKKVVLVGRGKLVGAPL
                     EKALRASEIDVEVADRKTPSLADKTREADIIITATGSPAILYPDMIKQGAVVVDAGVA
                     SEDGKTVGDVAPEVYARDDLTITPVKGGVGPLTVCALFENVIRAALRTVE"
     gene            complement(4904..5317)
                     /locus_tag="VN778_02700"
     CDS             complement(4904..5317)
                     /locus_tag="VN778_02700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49912.1"
                     /translation="MNDQKVIQFNATAGSYFVVFLVTAIMAYIPLFGWAFSFNYMNEW
                     LANNSLVNGKKVAYKAGYGETLKFLFINLLLLLITLGIYIFWFAPKSYRYAADHVSYV
                     DGATPAPASFAPPAAPSPPTAPQPPVNPTPPTVVQ"
     gene            5428..5504
                     /locus_tag="VN778_02705"
     tRNA            5428..5504
                     /locus_tag="VN778_02705"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5462..5464,aa:Lys,seq:ctt)
     gene            5577..5921
                     /locus_tag="VN778_02710"
     CDS             5577..5921
                     /locus_tag="VN778_02710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49913.1"
                     /translation="MDKKSDNTIGGHLSKARAYTILILKKGPKYQEDVENGKPIVWEH
                     GKRNMALREDGVLAVVCPIIDDSEYAGIGVFNRTMEEVRKIMDDDPAIKAKVLTYGVY
                     SGKGFPGDMLPS"
     gene            complement(5965..6384)
                     /locus_tag="VN778_02715"
     CDS             complement(5965..6384)
                     /locus_tag="VN778_02715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49914.1"
                     /translation="MNRSLKYLLPLLVVVVLNYLAQIPYYFHQYYIGRHLAPSWPGTV
                     LLLFTFLWFLAGYTRFVTSKQYGVGLLLSFLAAQVLFYGHAIIFGLINGGGAVAQLKT
                     HSPFLFVIFLIGYINFGVAAYYLVRLLKDKSNANTVQ"
     gene            6526..7167
                     /locus_tag="VN778_02720"
     CDS             6526..7167
                     /locus_tag="VN778_02720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49915.1"
                     /translation="MKHPNVPYHGNPTNSFCALACYTMVGQYLLPDAGITFEMFGELA
                     DYKPGYVVWAYPVWKWMLDHGVHIIDQDISGQEEWSKEGVEGLRRSVSKKEFEYYKMN
                     TYDLEAVTKQLQLVAEHPNFKSTQKRLSWDDIVEEFNKPGICDITLDGKFLSHKPDFS
                     VHRVVLLDITDNEVTFHNPRVDNGGAFQKETINHFRAAIEKLSGPEVCRYWLE"
     gene            7274..7726
                     /locus_tag="VN778_02725"
     CDS             7274..7726
                     /locus_tag="VN778_02725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49916.1"
                     /translation="MAQLTQTKPPLYPEQRAMGVFCLLWIAAYAFCLFAPLGSNKNPD
                     ASFGLFDALSAVIFTSSLALLAGIVASLIRKVGSRPGTMLAVSICGIIATLWCFVSLG
                     ASSIDCSAGEVCAGTASSSMQIILSAIVFVIWVPVPFIISRLFKARLS"
     gene            7914..8699
                     /locus_tag="VN778_02730"
     CDS             7914..8699
                     /locus_tag="VN778_02730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49917.1"
                     /translation="MNLQKLKSLFLKTLIGCLIAAAVLAVISVIIGHFNDLFFKSLFT
                     ILLIALHSLLSFSFIVNNEREETFDSLEFFTNVTFFIIVLSFITSILGVWKLFPGALV
                     ARLYALYFVLLFATLHGEVLAKTRGKQNNIDTIVYANYIFMAIVVLMLIPVIFLSDNS
                     TLPSLYYRSLAAAGIVDATLTLIAVILHKLYIQKHPTLTDNLYNVQQLPGAYGQNTQG
                     PQLAAAPPKKGMNIFVKILIGYVVLQLIGALFVVVMGALVFRR"
     gene            complement(8845..>9544)
                     /locus_tag="VN778_02735"
     CDS             complement(8845..>9544)
                     /locus_tag="VN778_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HXR49918.1"
                     /translation="WHAFLKTFHGALDALSPQCVASYAFGNHDQHRIVTRLGEPTARS
                     AAVLLLTLPGMAFIYNGDELGMKNGKIPPDMVQDPGAVGGEGRDPERTPLQWSPDKNA
                     GFTTADRPWLPVADNYKTHNVETESKDPDSFLSLYRQLGRLRNQSAAMRYGALELIET
                     GHTSVLGFARVSDEERHTILINFSNKPLKFTMKSKAPLGDFRLSSDPQTRLHIVKNGK
                     IEMQPHEAAVFARM"
BASE COUNT         2451 a         2370 c         2300 g         2423 t
ORIGIN      
        1 tggaaatgac gatgtcgcgt agtgctggcg catcttcgtg gttgtcatag tgtaccggga
       61 agccaccgcc gatattaagc acttctaaag taataccctt tttgagtaac gcctcaatga
      121 tcggctggac agtatgaata gcccgagccc acatatcatg gcgagcggct tgtgagccga
      181 catagaagct aataccatat gttttgaggc caagcttacg agcgtacagc aacatatcct
      241 taacagtatc cggtggcgtg ccaaagcgct cggacatcat gaagacgctg ccggtatcct
      301 ccacaatagt ccggataaaa accttggcgc ccggagcatg ttcggcgatt ttcttaacct
      361 cttctttaga atccgccgca aaccggtcta cccctgcttg atgcgccaag cgaatatgct
      421 ctgccagctt aattgaggta ccatagacaa tctttttagg atcgacgccg acttctagca
      481 acatagctat ttcataggct gaagcagcct caaaaccact gccaatctca ttaaggtagg
      541 ttagtatttt ggagtcggag ttcgccttca gcgcgtaata cacttcagcc ttatcaaaaa
      601 gatgggtgaa ggtgagatag tttttcctga ggatttcccg gctggtgaag aaaaatggcg
      661 tggtgtagtt cagaaattga ggcaagtgca ggacttgctc tggttcaatt gtttgtagtg
      721 tcattgtttt tattttattg ttgagcttca taagtgtagc aggccataag cactttaatc
      781 aagaggattg ctctactgta aagaaaccag cacaatcccg aggatagtga gccccaccgc
      841 gccccattgg agtttactaa gacgctcctt taggaagatg taagccagca ccagcgtcgg
      901 taaagaaaag gcgccgctca ccagtgccac aatactggtc aagctagtgg cgctataacc
      961 ccagctaata gccagataag ccacggtttc gcccagaccg atcagcacca agaacttcca
     1021 aagctcccgc ggtactcccc gcagactgct tcgcataagc ttagcgagtg ccacggcagc
     1081 tagtagcata aacgcataca tactaagcgc gtacggcaag gcctggcggc cacttatgaa
     1141 gcggtcccag ccgatggtcc aagccgctgc cagtattgta gccagcgcaa tttctttgag
     1201 acccggaata ctattgaagc gcttggagcg acgcgttccg gtgggatcaa tattaataag
     1261 taaaatgccg ccgaagagta gcaccagggc tatagccaat cccgccgaaa gcgtttcgcc
     1321 gaaaaaaatc accgccgcta cagcaacgag gcccggaaaa gaggcaaaga tcgggttcag
     1381 cacggccagc tggccctttc caaaggcttc gtaagccagc caatagacaa tcatctggag
     1441 ggcgccgaaa aacgccgacc cggcccaggt cccgaagcta ttaggaaatt gcaggtgccg
     1501 gccgaacagc caggcatcgc ctagggcaat aatgataaag agggtggtgc caaaagcgtg
     1561 ggcccacacc aagcttttga tcgctcccac agcgtcggtc gttttcttgg cacagaagtc
     1621 ggcaaaaccc cagccaatca taccgcctaa accagccagg atcgcgatcg tcaattgatt
     1681 atgcatattt tgttattttg gctctataat acatagtagc aaggtcacta atgtaactat
     1741 agcagcaatc aatgcccaaa atcgccataa tcgaagacga caaagccatc tctcaaatgt
     1801 accggctgaa gtttgagacc gaaggcttca ccgtccaaac agccgaaaac ggcaagctcg
     1861 gcttggagct ctgtgagcaa atgaagcccg acatggtgct cttggacctc atgatgccgg
     1921 aaatgaacgg cgatgagatg ttagctaaat tccgcgccac cgactggggc aaaggtatcc
     1981 aggtaattat tctgaccaat atgggcgagc aggaaattcc gccggaagtt aaagccctgg
     2041 gtgtcagtgc cgttatcttg aaggccgaca tgaccccgcg gcaagttgcc gacgtcgtta
     2101 aaaaactctt ggctccggca taaatcttat gaaaatcgcc attttaggct acgacgttga
     2161 gggccgggcc tcttacgaat acttcaaagc gcagggccac gagctaacca tttgcgacca
     2221 gaatccggcg atcgcgattc cagagggcgt gccagctgtc ttgggcgaaa actaccttga
     2281 cgaactggac cgttttgact tgctggtcag aactgccggt ctgacaccaa gtaaaattct
     2341 cgaaaagaat cccgctgtag cagataaaat taccactcat attaatgagt tcttccgtgc
     2401 gtgtccgact aaaaatatca tcggcgtcac cggcaccaaa ggcaaaggca cgacctcgac
     2461 tttaattacc aagatgctcg aagctgacgg caaacaggtg cagcttggcg gcaatattgg
     2521 cgtaccgccg ctcacctttc tcggcgagct gacggcagag tcgtgggtag tgctggagct
     2581 ttctagtttc cagctcatcg atatacggta ctcgccactg attgctatct gcctgatggt
     2641 cgtgcccgaa cacctcaact ggcactctgg tctcaacgac tatacggccg ctaaatccaa
     2701 tctgttcgcc catcaaacag ccgaggacaa ggctatttat tttgccgaaa acgacctctc
     2761 taaactgatt gccggacatg gtccggggca aaaaatccct tactttgctc cgcctggagc
     2821 ctttgttgac aatggcgctg tcagtataga tgggcaagtg atttgtcagg tcagcgagct
     2881 tcagctgcgc ggcgcccaca actggcaaaa cgtctgcgcc gccgtgaccg ccgcctggca
     2941 aatcgtgcaa gatgtcgatg ccattcgttc cgttttgacg acctttagcg gcctcgagca
     3001 tcgcctacag ctggtgggcg aagtggcggg ggtgaaatac tacgacgaca gttttggcac
     3061 tacgccggag acggccgtcg tggctattga agcttttacc gagccaaagg tcttaattac
     3121 cgggggcttc ggcaaaagca ccgatttcga agctctgatt gatactatcc tgaaaagcga
     3181 cgtcaagcat gtcgtctgca tcgggaaaac cggatctgtc attgccgacc gcttggaggc
     3241 ccgcaaggag gaacgctgcg tcacctacac ggtctggtct tcctatgacg cccttatcat
     3301 gcctgaaatc gtcgaggctg cccggcaaca agccgatcct ggcgatgtag tcttgctagc
     3361 cccggccgac gccagttttg acatgttcaa agactataaa gatcgcggtg cgcagttcag
     3421 ccaagccgta caagctctcg ccacaaacgc ttaatcaccg cttgctccgg ctgcaaaaca
     3481 cgagcctgcc cggcgaccag cgaagctatc tcttcctcga tccagtggta gtgcgtacag
     3541 cccagcacga tgacatcagc tttttgattc agtacttcat taatccggtc agcaattttc
     3601 tgcttatcga tttgcttttg ctcgatcata taggcccagt cgctgcaatc cggctctagc
     3661 acgacgacat cttgagcata cgtggtcttg agccattcgt aacgcggact cagtagcgtt
     3721 gtcggcgtcg cacagacggc aatcacccca cttttagtca gctgtgctgc cggcttgacc
     3781 attggctcaa tgccaatgag cggtactgcg aaacgagcgc gtaactcctc gatcagcgtc
     3841 gtcgatacgg tattgcaggc aacaacaatt acttgacagc cagcgtcaac gagctcctga
     3901 aatatcggca ccaccaagcc gagaatttcc tgtggtggcc gtatgccgta tggcacgtgc
     3961 tccttatcct cgcgcagtat gacctcgtgc tccggcagcg ccttacggat agcatttgcc
     4021 accgacagac ccccaactcc cgaatcaaag accccgattt tcattcgacc gtgcgcagcg
     4081 cggcgcgaat aacgttttcg aacagcgcgc acaccgttag tgggccgacg ccacctttaa
     4141 ccggcgtaat tgtcagatcg tcgcgcgcgt aaacttctgg tgcgacgtcg ccgacggttt
     4201 tgccgtcttc gctagctact ccggcatcga ccacgacggc accctgcttg atcatgtcgg
     4261 gatataggat ggcggggctg ccggtagccg tgataataat gtcggcttca cgagttttgt
     4321 cggctaagct tggcgtcttg cgatcagcca cctccacatc aatctccgaa gcgcgcaaag
     4381 ccttctccag cggtgccccg accagtttgc cgcggccgac tagcacgact tttttaccgg
     4441 cgagatcgac attgtaaccg gacagcaacc acaggattgc catcggggta gccggctcaa
     4501 acaaagcgcg ctcccccagt gcgtcaacgt ccttttcggg tgctacgaga ttaactattt
     4561 tatcagtttg ctcgggtttt tccagaggta actgtacgat tactccatgc acgcttttat
     4621 caatacttaa cttgttaagt aattcttctg tctctgtttg tttgacacga tgcacgtcaa
     4681 cgtcgatcaa aatgtcagcg ccatattgct gctttagccg tatgtatagg tctatcactg
     4741 gattgtcgat tgtcaccaca atagccagct ttggctgcac accatgcgcc tgcctcagcg
     4801 cccgcacttg atgcgcctgc cgctctttaa taaagccagc taactcactg ccattcagta
     4861 atttcattag ccttatttta acagagagtg gcggatatag cgtctactgg acgacggttg
     4921 ggggcgtggg attcacaggg ggttgcggcg cagtcggagg gctaggggcc gcgggcggtg
     4981 caaaggatgc tggcgctgga gtcgctccgt caacataact gacatgatca gcggcgtaac
     5041 gatagctttt cggtgcaaac cagaagatat aaatgccaag cgtaattaaa agcagcaaca
     5101 gattgataaa gaggaatttc agggtttcgc catagcccgc tttgtaggct accttcttgc
     5161 cgttgacaag cgaattatta gccagccatt cattcatata gttaaagcta aaggcccagc
     5221 cgaacagcgg tatgtaagcc ataatcgccg ttaccagaaa aaccacgaag taactacctg
     5281 ccgttgcatt aaactgtatt actttttgat cgttcatctt gtcatcgtcc ttatggttag
     5341 gtttatggct ataatatcac aacgaatgac tttgctctcc agttacttgc caaaacagag
     5401 gtaatttgct aaagtttcct atgaatcggg ccattagctc agctggttag agcacctgcc
     5461 tcttaagcag ggtgtcctgg gttcaagtcc cagatggccc tccaagtatt ccacacaacg
     5521 atattattga aatgagaagc cgcaaaaggg tttctcattt ttgttatagt tagataatgg
     5581 ataaaaaatc tgacaataca ataggcggac acttaagcaa agctagggcc tacaccatcc
     5641 tcattctgaa aaagggtcca aaataccaag aagatgtgga gaacggcaaa ccaatagttt
     5701 gggagcacgg caaacgcaac atggctttac gagaagatgg agtactggct gttgtttgcc
     5761 caattattga cgactcagag tacgcgggca ttggggtatt caatcgcacc atggaagaag
     5821 tcagaaaaat aatggatgac gacccagcta ttaaagccaa agttttgacc tacggcgtgt
     5881 actctggaaa aggctttccg ggcgatatgc tgccttcttg atttttccga tactgtacgc
     5941 aggccactcc tcagtaccag ccactcattg aacagtgttc gcgttgcttt tatctttcaa
     6001 taatcgtact aaatagtagg cggcaacgcc gaagtttata tagccgatga ggaaaattac
     6061 aaatagaaat ggactgtggg tttttagctg ggctacagca ccacctccat taatcaggcc
     6121 gaagatgatg gcatgcccat aaaacagcac ctgggccgcc agaaatgata gtagcaagcc
     6181 cacgccgtac tgcttgcttg taacaaatct agtatatcct gccaaaaacc atagaaacgt
     6241 gaatagcagc aaaacggtgc caggccagga cggtgccaga tgcctgccga tatagtattg
     6301 gtgaaagtag tagggaattt gggctaagta gttgaggact acgacgacta gaagaggaag
     6361 aagatatttc aagcttctgt tcataatata tatatgatta tagctgaaca ataaacagag
     6421 ttaggcatgc tgcaatttag ttgcaacagt caacactaag gactctcata ttgcttatgc
     6481 ttataacaga agtaaatacc tctgtttttt tgctattatt gtgttatgaa gcaccctaat
     6541 gttccctatc atggcaatcc tactaattcc ttttgtgcgt tggcatgtta cacaatggtc
     6601 ggacagtact tgttgccaga tgcaggaatt acttttgaaa tgtttgggga gctggccgac
     6661 tataagcctg gttatgtagt ttgggcatat cctgtttgga aatggatgct cgatcacgga
     6721 gttcatatta tcgaccaaga tattagtggc caagaagaat ggtcaaaaga gggtgtagaa
     6781 ggactcagga gatcggtcag caagaaagag tttgagtatt acaaaatgaa cacctatgat
     6841 cttgaagccg taacaaaaca attacaactt gtagccgagc accctaactt taagagcacc
     6901 caaaagagat tatcgtggga tgacatcgtt gaggagttca ataaaccagg catttgcgat
     6961 attacactcg atggaaagtt tctaagtcac aaacccgact tttcagttca ccgtgtagtc
     7021 ctacttgata tcacagataa tgaggttact ttccacaatc ctcgtgttga caatgggggt
     7081 gctttccaaa aagagacaat taatcacttc cgtgccgcaa tcgagaaact cagtggaccg
     7141 gaagtatgtc gatattggtt agagtagttg cgataacgtc tactaaaccc ctccaactat
     7201 tctttacaaa agattattag attgaaaatt atacaagctg gtttatagat gttcttaatg
     7261 tatacttatc tacgtggcac aattaacaca aacaaagccc ccactttatc ctgaacaacg
     7321 cgcaatggga gttttttgtt tactttggat agctgcttat gcattttgtt tgtttgcccc
     7381 tcttgggtct aataaaaacc cggatgctag ctttggatta tttgatgcat tgagcgcagt
     7441 tatctttacg tcttcgcttg cactcctcgc tggaatagtt gcttcgctca tacgcaaagt
     7501 tggtagcagg ccaggcacta tgctggctgt atcaatttgc ggcattattg ccactctttg
     7561 gtgtttcgtt tcacttggtg cgagcagtat tgattgtagc gcaggcgagg tttgtgcagg
     7621 gacggcttca agttccatgc aaattatttt gtctgcaatt gtatttgtta tatgggttcc
     7681 tgttccattt atcataagca gactctttaa agctcgattg tcataaaaac caggcctagt
     7741 aagcgcttaa gattttcgca cctcgctgcc aaatgacgta tggttctacg ctcgaagcca
     7801 agggcggccc atctgctcct ctttggccga gctgtgaagg cacctatcaa aaataatcat
     7861 aagcattttg acctctagta gctttattgt atagtgatta ccaagagttt attatgaatc
     7921 tgcaaaagct taaaagtctc tttctcaaaa cattgattgg ctgccttatt gctgctgccg
     7981 tactggctgt aattagcgta ataatcggcc acttcaatga tttatttttc aaatcgttat
     8041 tcactatatt attgattgcg cttcatagct tgttaagctt cagctttatt gttaacaatg
     8101 agagggaaga gacctttgat agtctcgagt ttttcacaaa tgtaaccttc tttatcattg
     8161 tcctgagctt cattacctcc atccttggag tatggaaact gtttcccgga gcgttggtgg
     8221 ccaggctata cgccctctat tttgtacttc tttttgcaac tttacatggc gaagtactgg
     8281 ctaaaaccag gggtaaacaa aacaatattg ataccattgt ctacgccaat tatatcttta
     8341 tggcaattgt agttctaatg ttgattcccg tgatcttcct gagcgataat agtacgctgc
     8401 cctcgctgta ttatcggtca ctagcagcag ctggcattgt tgacgctacc ctaacgctca
     8461 tagctgttat cttacacaaa ctttacatcc aaaagcaccc taccctcacc gataatctct
     8521 ataatgtgca gcaactaccg ggagcgtacg ggcaaaacac tcaaggccct caactcgcag
     8581 ccgccccacc taaaaagggc atgaatatct ttgtgaagat tttaatcggt tacgtcgtac
     8641 tgcagcttat aggcgcactg tttgttgtcg ttatgggtgc gctggtgttt cgtcgctaac
     8701 gcaaggcata gccgtaccac cggggagtaa ccaaaggagc gtctgggttc catactctcg
     8761 agcgcaagcg tgccttggtg ggtcgccgcc agtcgtatag gattccggcg agcttacctg
     8821 attttttagc agttttactc ccggctacat cctagcaaaa acggcggctt cgtgcggctg
     8881 catttcgatt ttgccattct tgactatgtg cagccgagtt tgcggatcgg aagatagcct
     8941 gaaatcgcca agcggcgctt ttgacttcat tgtaaatttc aatggcttgt tcgaaaagtt
     9001 aatgaggatg gtgtgtcgct cttcgtcgga cacgcgagca aagccgagga ctgacgtatg
     9061 ccccgtttcg atgagctcta gggcgccata gcgcatagca gccgactggt tacgcaatcg
     9121 tccgagctgg cgataaagtg agaggaaaga atcgggatct tttgactcag tctcgacatt
     9181 gtgtgttttg taattgtcgg ctaccggtag ccacggcctg tcggcagtgg tgaaaccggc
     9241 gttcttgtca ggcgaccact gcagtggagt ccgctccggg tcgcgcccct cgccacctac
     9301 ggcgccagga tcttggacca tgtcgggcgg gattttcccg tttttcatgc ctagctcatc
     9361 accgttatat atgaacgcca tgcccggaag cgtcagcagc agcacagcgg cactgcgagc
     9421 ggtcggctca cctaagcgtg tcacgatgcg atgctgatca tgattgccaa aagcgtagct
     9481 tgccacgcat tgagggctta acgcgtcgag ggcgccgtga aaagtcttaa gaaatgcatg
     9541 ccaa
//
LOCUS       DATNGP010000109         2754 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_963799, whole genome shotgun sequence.
ACCESSION   DATNGP010000109 DATNGP010000000
VERSION     DATNGP010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2754
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_963799"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(224..424)
                     /locus_tag="VN778_02740"
     CDS             complement(224..424)
                     /locus_tag="VN778_02740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49919.1"
                     /translation="MSKPVTAIMQKEMTRQEFLMTLGFGIATVLGFSNLIRLLTGKSA
                     ESHIHSRNGLGYGTSPYGGGKE"
     gene            complement(506..1615)
                     /locus_tag="VN778_02745"
     CDS             complement(506..1615)
                     /locus_tag="VN778_02745"
                     /inference="COORDINATES: protein motif:HMM:NF013097.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HXR49920.1"
                     /translation="MKTKKKSTHNRRTAPHSRRDSQKTQPFIEHAYELRRRLYYIAVF
                     VVAWGSATYAVQQHVVNILLKPAHGQHFIYTSPGGGIDFLFRICVYSGLVLSTPVIVY
                     NILEFFEPLMSRASRRFIAINSMVCGLLALAGVAFGYFIGLPAALNFLFHQFTTAQIK
                     PLVTIQSYLGFVIAYLLGSALLFQLPLILVCINRIKPLKPQRLFRYERWVILAAFVLA
                     GLMNPTLNLLSQLLVAGPFILMYQIGIALIAFINRPRRQRAAEPRLQRSVPVQAAPDP
                     RLARSMPAASAAVAAAQIQAITRPPASTIVMIADIHPRQASPSTPRSFMPRERLVART
                     APPAPRPSARYASLHRNEQRFMDFIQPSSKLFAPE"
     gene            complement(1566..1775)
                     /gene="tatA"
                     /locus_tag="VN778_02750"
     CDS             complement(1566..1775)
                     /gene="tatA"
                     /locus_tag="VN778_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013014176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HXR49921.1"
                     /translation="MLSNFAFLDLGAPELIIILAIILLLFGGKKLPELSKSLGESMRE
                     LRKGISGDVNENKKEVNPQQENSSS"
     gene            1876..2139
                     /locus_tag="VN778_02755"
     CDS             1876..2139
                     /locus_tag="VN778_02755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49922.1"
                     /translation="MSSLYIFAQTYGSGGYDSSAYNASSTTGTGSSGGSGTSTPGATG
                     TSGGTLTNTGFDILLASSLACAIIFAALLVRFWKRPALEREDS"
     gene            complement(2245..>2754)
                     /locus_tag="VN778_02760"
     CDS             complement(2245..>2754)
                     /locus_tag="VN778_02760"
                     /inference="COORDINATES: protein motif:HMM:NF016119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class E sortase"
                     /protein_id="HXR49923.1"
                     /translation="AMFHLENHGTKQKQLLAEVQAKASPHTSQAPGTPAGNRVIIPSM
                     LLDQPILEGSIANQYKTLNQGIWRWPRGSTPDKGGNTTLIGHRFTYTNPRGVFYFLNK
                     VKIGDEIGILWNNHKYVYKVATITEVSPHDTKIEDSTTNSELTLFTCTPLWLPKDRLV
                     IVANEETAT"
BASE COUNT          694 a          629 c          730 g          701 t
ORIGIN      
        1 tggtaggcgc gccatagtgt ctaattttga accagttctt attattgatc aattactaga
       61 gagagtcaat ttgcacaaat aggccaatgg aatggtcgcc tgaaggggct atttttgttg
      121 cccacgccat tgtttcgcat agtattagaa gtgatttctg gtgatataaa aaatgcgaag
      181 attagctgaa atgtcggcgc ttctctatct tggcgggtga cggctactct ttgccgccgc
      241 cataagggct ggtaccgtag cctaggccgt tgcgtgagtg gatatggctt tcggctgact
      301 tgccggtcaa gaggcggata aggttggaaa aaccgagcac cgtggctata ccgaagccca
      361 acgtcatcag gaattcctga cgggtcattt ccttttgcat gattgctgtt actggtttgc
      421 tcattttgtt tgatttttat ttttacgtta ctaaagttga gtatagggaa ccgtactgac
      481 ggtgtcaaga aagccaccgc agttattatt cgggagcaaa caatttgctg ctaggctgaa
      541 taaaatccat aaaacgttgt tcattcctgt gcagtgacgc gtagcgggcg gatggccgtg
      601 gggcgggtgg tgccgttcga gcgacaagcc gctctcgtgg catgaagctt cgtggggtag
      661 agggagaggc ttgccgtgga tgaatgtcgg caatcattac tatagtcgat gccgggggac
      721 gagtaatagc ctgaatttgg gcggctgcca ccgctgccga agccgctggc atacttcgag
      781 ccagacgcgg gtcgggtgca gcttgaacag ggacggatcg ttgcagtctg ggctctgccg
      841 ctcgttgtct tcgcggccgg ttaatgaaag caatgagtgc aataccgatc tgatacatca
      901 agatgaatgg cccagccacc agcaactggc tgagaaggtt gagtgtcggg ttcatcagac
      961 cggcaagtac aaaagccgcc aaaataaccc agcgctcata gcgaaacaga cgctgcggct
     1021 tcaaaggctt gatgcgattg atgcacacca ggataagcgg tagctggaag agaagagccg
     1081 atccaagcag ataagctatg acgaagccga ggtatgactg aatggttact aagggcttga
     1141 tctgtgcagt ggtaaattgg tggaacaaaa aattcagagc ggccggcagc ccaataaagt
     1201 agccaaatgc cacacccgcc agtgccagca gaccacagac catgctattg atggctataa
     1261 agcgtcgtga agcccggctc atgagcggct caaagaactc caagatgtta tagacaataa
     1321 cgggtgtact cagcaccagg ccgctataga cacagatgcg gaatagaaaa tcgatgccgc
     1381 cacctggact ggtataaata aaatgctgtc cgtgagcggg cttcagtaag atattgacga
     1441 catgctgctg aaccgcatag gtagcgcttc cccaggcaac gacaaaaacg gcgatgtaat
     1501 ataatcgccg ccgaagctcg taagcatgct caataaaagg ctgggtcttt tggctgtccc
     1561 gccgactatg aggagctgtt ctcctgttgt gggttgactt cttttttgtt ttcattgacg
     1621 tcgccgctta tgcctttgcg tagctcacgc atcgattcac ctaaactttt ggaaagctca
     1681 ggcaattttt tgccgccgaa caagagcaat ataatggcca aaattatgat caattctgga
     1741 gcgcctaagt ctaaaaatgc gaaattgctt aacatgtccc cttaacctca ctatttttaa
     1801 cttttaattt tctcataccc tctattatgg tatcataagc ataataaacc atacgaacaa
     1861 aaaaagaaac aaacaatgag ctccctctac attttcgcac aaacttatgg cagcggcggc
     1921 tatgactctt cagcctacaa tgcttcgtcg acaactggca cgggttcatc gggcggctcc
     1981 ggcaccagca ctccaggtgc aacgggcaca agcggcggca cccttacgaa taccggtttc
     2041 gacatcctat tagcatcgtc tctagcgtgt gccattattt ttgcagcctt gttggttcgg
     2101 ttttggaagc ggccagctct cgaaagggaa gacagctaat ttaagcccca agcaacacct
     2161 tattgaggtt gctatttaag gtcaaaataa gagcaatgct gatgaggagc aagatcagca
     2221 gcagccaaaa ctttactttg tcgctcatgt cgctgtctcc tcgttggcta cgatcaccaa
     2281 acgatctttg ggcagccaca gcggggtgca cgtaaagagc gttagctcgg agttcgtggt
     2341 actgtcttcg atcttggtgt cgtgagggga gacttcagta atcgtggcta ctttataaac
     2401 gtatttatgg ttgttccaca aaataccgat ttcgtccccg atctttactt tattaaggaa
     2461 gtaaaaaacg ccgcgggggt tggtgtaggt aaagcgatgc ccgataagtg tggtgttgcc
     2521 gcctttgtcc ggcgtactgc cgcgaggcca gcgccagatg ccttggttga gtgttttgta
     2581 ctgattcgcg attgagccct ccaatatggg ctgatcgagc agcatacttg gtatgataac
     2641 tcgattcccg gcaggtgttc cgggagcttg gctggtatgg ggcgaggctt tggcttgaac
     2701 ttcggccaaa agttgttttt gcttcgtacc atgattttca aggtgaaaca tggc
//
LOCUS       DATNGP010000110         4084 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_1852207, whole genome shotgun sequence.
ACCESSION   DATNGP010000110 DATNGP010000000
VERSION     DATNGP010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4084
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1852207"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1009)
                     /locus_tag="VN778_02765"
     CDS             complement(<1..1009)
                     /locus_tag="VN778_02765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49924.1"
                     /translation="MAVSLLLAIAMLASVGFNLARPVQTSAAVADTINFQAKLETSDG
                     AIANDGDYNVEFKIYNAASGGTALWTEDYLNSASHGVHVVNGYLTVNLGSITAFPSTM
                     AWDQSLYITMNIGGTGGSASWDGEMSPRLQLTSVPYAFQAKSASQLQVSSGGNVGTLS
                     FTTPTTTDNILLPDASGTVCLDNSASCGFASSSGSNAYIQNNTVLQGNANFDIQSSAT
                     GSATGVIEALTGQTADLLQFRDSSANPISGFNSSGQLYYQSGSFTGTLAQNSLGQNTV
                     YHLPDPGGSSATICLSTGNCAGSGGGITGSGTTNFVARFNSSSTINASSLLYDNGSYV
                     GV"
     gene            complement(1646..1954)
                     /gene="rplU"
                     /locus_tag="VN778_02770"
     CDS             complement(1646..1954)
                     /gene="rplU"
                     /locus_tag="VN778_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016655224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HXR49925.1"
                     /translation="MKKAVIQTGGKQYLVAEGDTVNVELLKGAGKTAEFDALLVIDGE
                     SVQVGVPTVAKAKVTADVVEADKQADKVTAIRYKAKKRVHKVRGHRQHQTVLKIKKIA
                     "
     gene            complement(2113..>4084)
                     /locus_tag="VN778_02775"
     CDS             complement(2113..>4084)
                     /locus_tag="VN778_02775"
                     /inference="COORDINATES: protein motif:HMM:NF012361.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HXR49926.1"
                     /translation="GVVHLAPAYGEEDYELAKEHGFPIISNIDDNGFYSEGEWQGQYI
                     WDANKQIAKTLHERGVIWKIDYIQHSYPHCHRCGTKLMYRAHPSWFMDIEGQRQQMLE
                     QNGPIDWFPTHIKHGRFPKTVESAPDWNLSRDRFWATAIPVWKGRDSAGNEQVKVIGS
                     YAELKELSGVELEDYHRPWIDDVTFTIEGVEYKRIDKVMDSWFEAGSMPYAQFHYPFE
                     NAEKFEENFPGDFIVEYVGQVRAWFYYLHTLSVGLWGKNAFKNVIVTGTIAGNDGRKM
                     SKSYGNYTDPNELVDRYSADAYRFLLLSSPLLNGEDYALQDKDVADVQRKLAMVWNMY
                     DFFTLYADVDGWDSGLQEGELPTDPTPELSNPLDQWIVSRVHQLNHEIDDHMKRYDLP
                     NALKPILPFIDDASNWYVRRSRRRFWKSGDDADKAAAYRTLHYVLVHLSLLLAPFTPF
                     MAEELYHQLVGGESVHLLDWPEVGHVNELLLNVMREVRLGIEQGLAQRAAFNLKVRQP
                     LPFAKLYVTRLPDEHQRSDLEQMIKDELNVKQVALEETTSPYSPGDRSMTELTTDVTP
                     ELKREGLMREIIRHVQQARKQAGLEVDDRIQLALETADEEITAVLTDERLAQTIKQET
                     LAENLEEGAVSGFESQVKVEGAELKISLVKVT"
BASE COUNT          916 a         1101 c         1003 g         1064 t
ORIGIN      
        1 tgacccccac atagctgccg ttatcgtaca gcagcgaaga ggcgttgata gtgctggaac
       61 tgttgaagcg agccacgaag ttggtagtac cactaccggt gataccgccg ccgctgcccg
      121 cacagttacc ggtgctcaaa cagatagtag cgctgctgcc acccgggtct ggtaagtgat
      181 agacggtgtt ttgccctaga ctattctggg ccaaggttcc ggtgaagctg ccgctctggt
      241 agtacaactg gccgctgcta ttgaagccgc tgatggggtt ggctgagctg tcgcgaaact
      301 gcagtaaatc ggccgtttgt ccggtcaacg cctcgatgac cccggtcgcc gaacccgtcg
      361 ccgagctctg aatgtcgaag ttggcgttcc cctggagcac ggtattgttc tggatgtagg
      421 cattgctgcc gctggacgaa gcgaaaccgc aggacgcact gttatcgaga cacaccgtgc
      481 ccgaagcatc cggcagcaga atgttatcgg ttgtcgtggg cgtggtgaag ctgagcgttc
      541 ccacgttgcc tccgctgctg acctggagct gactggcgct cttggcctgg aaggcgtacg
      601 gcacagatgt cagttgcaaa cgtgggctca tttcgccgtc ccagctcgcc gatccacccg
      661 taccaccaat gttcatggta atgtacaagc tctggtccca ggccatggtg ctcggaaaag
      721 cggtgatgga accgaggtta acggtcaggt agccgttaac gacgtgcacg ccgtggctgg
      781 cgctgttgag gtaatcttcc gtccagagag ccgtgccccc actagccgca ttgtagatct
      841 tgaattccac gttgtaatca ccatcgttag caatggcgcc atcgctagtc tccagcttgg
      901 cttggaagtt gatggtatcg gcgactgcag cgctggtctg aaccggtcgc gccaggttaa
      961 agccaacact ggcgagcatg gctatggcga gcaataggct gacagccaag gctcggtgcg
     1021 cgcgcagcgc gcgtactccc gcgcgtacac gtaggcccgc gcgcgtacgc gtatagaaaa
     1081 taacaggggt caagagtaag cttcgacaaa cagcaaaaaa cacccttccc actcgggaga
     1141 tgagggtgcg tctgaacagt tctcgcttgt gcgatgtttg tcttcgcatt tgtattttga
     1201 tttagggatt tagttttttg tattggtttc tagccaaatg tgaggtttgg cggctcccta
     1261 gaggttgtcc agggagccgg tttggctttt tatttggttt gaaccaatgt ttgttggctc
     1321 agggaggagg ccgggattcc caagcttcaa ccctgtatct ccagtatcca cccattaaca
     1381 ggggtattca aggatttttt gaataaaatt tatatgcaat ttttactact cttttgctac
     1441 actttttctt aattttcgtt gtaataatac tgacgatcat gcgtctgacg ccttaatttc
     1501 ccctaaaagc ctctattttt ttggcttatc ccttatgctt tttgttgttt aaattataac
     1561 tgctctgaaa ttcctcaaaa cttatccaca agcggttcga agcaatttct gaataatcag
     1621 ggggttcggc tttgcctaag agggcttagg caatcttttt gatcttgaga acagtctgat
     1681 gctgacggtg accgcgaact ttgtgaactc gctttttcgc cttgtagcga atagccgtta
     1741 ccttatccgc ctgcttgtcg gcttccacga catcagccgt gactttagcc ttggcgacgg
     1801 tcggcacgcc gacttgcaca ctctccccgt caatcaccag taaagcatca aactctgccg
     1861 ttttgccagc acctttgagc agctcgacat tgaccgtgtc gccttcggcc acgagatact
     1921 gtttaccacc ggtctgaata actgcttttt tcatagaatt acaaagttac cgatagagca
     1981 tagcagaaat caccgcccct gtaaaggggg gtcccagacc tgaccctatt gccccacaat
     2041 ccagaaagta gtgtccacat tttatgggga cacaatgggg acactacttt tagaagagtg
     2101 cggcaaagct agttacgtta ccttgacaag gctgattttc agctcagctc cctcaacttt
     2161 cacctggctc tcaaaaccgc taacagcacc ctcctccaag ttctcagcta acgtctcctg
     2221 tttaatggtt tgtgccaatc tttcatctgt tagaaccgct gtaatttcct catcagccgt
     2281 ttccaaagcc agttggatgc ggtcgtccac ttccagcccc gcctgcttgc gagcttgctg
     2341 cacatgacga ataatctcgc gcatcagccc ttcccgcttg agctcaggcg tcacatcagt
     2401 cgtgagttcc gtcatggatc gatctccggg agaataagga ctggttgttt cctctaacgc
     2461 tacctgttta acattcaatt catccttgat catttgctcc aaatcagatc tctggtgttc
     2521 atcgggtaat ctcgttacat agagcttagc aaaaggtagt ggttgtcgca cctttaagtt
     2581 gaaagccgct ctttgagcaa gcccctgttc aatccctaaa cgaacctctc gcatgacgtt
     2641 aagcagcagc tcgttcacat ggccgacctc cggccagtcg agcaggtgca ccgactcgcc
     2701 tccgactagt tggtgataga gctcttcggc catgaagggc gtaaatggtg ccagcagcag
     2761 gctcaagtgg accaatacat agtgcagcgt ccggtaagca gcggccttgt cggcatcatc
     2821 gccactcttc caaaaacgac gccggctgcg gcgcacatac cagttactgg catcatcaat
     2881 aaacggcaag ataggcttca gggcattagg taggtcatat cgcttcatat ggtcgtctat
     2941 ttcgtgattt agctggtgca ctcggctgac gatccactga tcaagcggat tgctcagctc
     3001 gggcgtcggg tcggtaggta gttccccttc ttgcaacccg gaatcccaac catcaacatc
     3061 ggcatatagc gtgaagaagt catacatatt ccagaccata gctagtttgc gctgtacatc
     3121 ggccacatct ttatcctgta atgcgtaatc ctcaccattc agcaggggtg agctcagtag
     3181 caaaaagcgg taggcatcgg cactataacg atccactaac tcgttcggat cggtgtaatt
     3241 gccgtagctc ttactcattt tgcggccgtc attgccggca atggtaccgg tgacaataac
     3301 atttttgaag gcgttcttgc cccacagtcc gacggacaac gtatggaggt aataaaacca
     3361 ggcgcgaacc tggccgacgt attcgacgat aaagtccccc gggaaatttt cctcaaattt
     3421 ttccgcgttc tcaaatggat aatggaactg cgcgtatggc atgcttcccg cttcaaacca
     3481 agagtccatg accttatcaa tgcgcttgta ctctacgccc tcaattgtga aagttacatc
     3541 atcgatccat gggcggtggt aatcttcgag ctctacccca gataattctt tgagttcggc
     3601 ataactgcca atgactttca cctgttcgtt tccggcacta tccctgcctt tccagaccgg
     3661 gatcgcggtt gcccagaagc ggtcccgact cagattccaa tcaggcgccg actccaccgt
     3721 tttcgggaaa cggccatgct tgatatgtgt cgggaaccaa tcaatcggac cgttttgctc
     3781 aagcatctgt tggcgctggc cttcaatatc catgaaccaa ctgggatgcg cccggtacat
     3841 aagcttggta ccacagcggt ggcaatgcgg atagctatgc tgtatgtaat cgatcttcca
     3901 gatcacacca cgctcatgca acgttttggc aatttgctta ttggcatccc agatgtattg
     3961 accttgccac tcaccctcgc tgtaaaaacc attatcatcg atgttggaga tgatcgggaa
     4021 gccatgttct ttagctagct cataatcttc ctcaccgtat gccggcgcta agtggacgac
     4081 accc
//
LOCUS       DATNGP010000111         4486 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_1294331, whole genome shotgun sequence.
ACCESSION   DATNGP010000111 DATNGP010000000
VERSION     DATNGP010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4486
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1294331"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="VN778_02780"
     CDS             complement(<1..329)
                     /locus_tag="VN778_02780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49927.1"
                     /translation="MKKQLIRAAASAVLGLSLTTGFAAAQSCSITGPTGPQSNNQVHV
                     TSHNSADIDNDNNVSAWNNNSQHGSSGDARVSDNTTGGGATSGAVNNSSTTSTSASVN
                     NGSGAGMG"
     gene            complement(485..1057)
                     /locus_tag="VN778_02785"
     CDS             complement(485..1057)
                     /locus_tag="VN778_02785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49928.1"
                     /translation="MGLLNNHALHASTGRLLHEGSSSTPTFRPFNKTLVIGLALLLFA
                     VLILLADHSAGAPKQAAHSSALQITHSVSSSSEPANSGALNSNSMAHSSSSSHVSASV
                     NSSGNGQASTKVTVNGQDVPVPQNGSTQQTVTSPDGSSTHVSVSQSSSGNGQVDNSTS
                     TNTSLNVQSFSSSFDSSSTDSNVNIQENGP"
     gene            complement(1395..2216)
                     /locus_tag="VN778_02790"
     CDS             complement(1395..2216)
                     /locus_tag="VN778_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49929.1"
                     /translation="MRNQRPVRLLVTAVGFSLLFLGLTPVSASSANISHSFHADSTIS
                     NGSIVSLEQQKSDYVELANTSNGSRLLGVAVASTDSLLAVDPGQGTVQVATTGTASTL
                     VSTFNGAIKVGDQIAVSPFSGIGMEAAAGSRIIGLAQTAFDGTDSNAVTQQITDKSGK
                     TKQIRVGYVRLTIGVGSNPVDSGGEQLNSLQKMAKSLTGHTVSTARIIISLLVALVAT
                     VSLIVLIYASIYGSIISIGRNPLAKYAVFRTLSSVLGMAALTALVAASIIFFLLR"
     gene            2428..>4486
                     /locus_tag="VN778_02795"
     CDS             2428..>4486
                     /locus_tag="VN778_02795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49930.1"
                     /translation="MELENLKGDPSLEQSSNPAAYSSSLEGPADTTVTRTTTSSSSQP
                     LFRYNRLAVTISVLSLLVIAIIGGGSMWLLSSKGSNNPSSANSAGSSDNYAVSSLPLQ
                     GLKADTQLQVGEADHLSVNGQLKVSNTLVVSPTATPTSPVTGQIYYDQKTNQPYYYNG
                     TAFVSLAPTAVPQHVTSIGGVSGVISVGNGLQVTSGQLGLTSAITQKLGQSSGSGVSS
                     LTSGTSNLVITNTGNGNYTLSDNSVVNSGSTGQIALITGAQTIGGSILSQSGSSLTAG
                     GTLTVSGGLLTNSLQQTAAGNNVAISAGSDSIVFTDGSRTFAFPAGGGANQTICTTGV
                     TCASGGGIAVLLQPGAAQADTGAGSSIFVNNTGGGNLLELQGAGSDRFVVTNGGNVTA
                     GGTLTLGSALTVANGGTGGVSLTANGVLYGNGAGAVQATGAAANSILATNGSNVPGLT
                     QTLPIAVQSNITTTGILATGSIASGFGAISTSNNITTSAALQGGTLSVASGNFTINSS
                     GNVVTTGTATIQGAGGLSIGVAATTAGSLVFANASNGHLTTLQGLAPAGQDQTITIPA
                     SSAATDTVCLLTLANCVGAGGGVTASGGTQNFVPKFTNAGGTQLGNSLIFDNGTSVGV
                     NNSTPGASFKLDVGGNVNISGNTVVGTGLTVTAGGATVSAGGIAVTGNSTISGTLTSL
                     TGLT"
BASE COUNT         1045 a         1146 c         1288 g         1007 t
ORIGIN      
        1 cccatacctg ctccgctacc gttgttgacc gacgcagatg tcgaagtggt gctgctattg
       61 ttcacagcgc cgctcgtagc tccaccaccg gtagtattgt cgcttacccg ggcatcgcca
      121 gagctgccat gctgcgaatt gttattccag gcgctgacat tgttgtcatt gtcaatgtct
      181 gccgagttat gactcgtcac atggacttga ttatttgatt gaggtcctgt cggtccagtg
      241 atggaacagc tttgtgctgc agcgaatcct gtcgtcaggc tgaggcctag gacagcggat
      301 gctgcggcac ggataagttg tttcttcata ttacctcctt gccggtggtg ttgcgctcgt
      361 cttaagcgca acgcccttaa tagtatggtt agtttaataa aagtgctcca ggcgggttgg
      421 tcgcccggtg tcgctcaggc aggggtgtgt gcttgagcgg catcgagtgg ccaaaggata
      481 gttgtcaggg gccattctcc tggatgttaa cgttgctatc ggtggaagaa gagtcaaaac
      541 tgctgctgaa gctctggacg tttaggctgg tattggtaga ggtggaatta tcgacttggc
      601 cgttgcctga ggagctttgt gagacactga catgggtgga gctgccatcc ggcgaggtga
      661 cagtttgctg tgtggagccg ttctggggga cggggacgtc ctggccgtta accgtgactt
      721 tagtcgatgc ctggccatta cctgaactgt taactgaggc tgagacatgt gaggacgagc
      781 ttgagtgggc catgctatta gagttgagag ccccggaatt tgctggttct gatgagcttg
      841 agacagagtg cgtaatctgc aacgccgatg aatgagcggc ctgctttggc gccccggccg
      901 aatgatcggc taagagtatc agcacggcaa acaagagcaa agctaggcca ataacaagcg
      961 ttttgttaaa aggtcgaaaa gtgggggtgg aggaactacc ctcatgtagc agtcgtccgg
     1021 tcgatgcatg tagtgcgtgg ttgttcagta gtcccatttc atccctcctc acacgggccc
     1081 cacccaaaag ggctcgcacg gtcatcatag gcggctaatg caggcacatt ctgtgaaaat
     1141 tctaacaagc ccataaagtt gtaataacca aaatgtgttg gctaatgaat tgacagtggt
     1201 cagaaaaacg ctaatgcttt aattctgcat tgccgtaata aatacctagt tcaattttgt
     1261 gaaggtcgga cggctaaaat cgtggagagc gtggagaggt gcagcagggg aaatttttgg
     1321 ttcatttacg cgatgcgatt tcgacaacat aatttgtcat tttgtatctg ttaaattgat
     1381 actgagtaca cgagttaacg caataagaag aaaataatcg aagccgccac cagtgctgtc
     1441 agagccgcca taccgagaac ggaactcaag gtgcggaaga cagcgtattt ggccaagggg
     1501 ttgcggccga tcgagataat gctgccatag atggaagcgt aaatcagtac gattagcgac
     1561 acagtagcta cgagcgcgac aagcaggctg atgataatgc gggccgtgga aaccgtgtgg
     1621 ccggtgagag actttgccat tttttgtaaa ctattaagct gctcgccccc gctgtcgaca
     1681 gggttgctgc caacgccgat ggtgaggcga acgtacccga cgcggatttg cttggttttg
     1741 ccggacttat cggttatttg ctgtgtaacg gcgttgctat cggtcccgtc aaaggcggtt
     1801 tgggcaagcc cgatgatccg agaaccggca gctgcttcca tgccgatacc gctaaacggg
     1861 gaaactgcta tctggtcacc aaccttgatc gcaccgttaa aggtactcac cagcgtgcta
     1921 gccgtaccgg tggttgctac ctgcacggtc ccttgaccgg gatcgacggc cagcagcgaa
     1981 tcagtgctgg caactgctac cccgagtaat cgcgagccat tacttgtgtt ggcaagctct
     2041 acataatcgg atttttgctg ctccaggctg acaatgctac cgtttgagat ggtgctatca
     2101 gcatgaaaag agtgggaaat gttggccgaa gaagcgctca ccggggtcaa gcccaaaaag
     2161 agcagactga agcctaccgc tgttaccaat aacctcactg ggcgttggtt gcgcatactg
     2221 gttacgatta tagcaacttc tagggcataa acccataaag ccttttaccg tacggcgcct
     2281 agaagccaca ttcttgacag gggtgggggt gtggaaaaat tgtatcaaaa aagtattcaa
     2341 tttacttgac ctctgtagca taagaggttt acaataaact tacggcaaaa tataccaata
     2401 agtataaata aacagcaata aacagagatg gaactcgaaa acctcaaagg tgaccctagt
     2461 ttggagcaaa gctctaaccc tgctgcttat agctcgagtt tggaagggcc ggccgatact
     2521 accgtaacgc gcacgacgac cagtagtagt tctcagccgc ttttccgcta caacagattg
     2581 gcggtgacta tctcagtgct ttcgctgctg gtaatcgcta ttatcggtgg cggcagcatg
     2641 tggctgttga gcagcaaggg gtcaaataac ccctcatcgg ctaattccgc aggctcgagt
     2701 gacaactacg cggtcagtag tttgcctctt caagggttga aggcggacac gcaacttcag
     2761 gttggggaag ctgatcacct gtcagtaaat gggcagctca aagtcagtaa taccctggtg
     2821 gtatcaccga ccgccacccc taccagtccg gtcaccggcc aaatttacta cgaccaaaag
     2881 accaaccagc cgtactacta caacggtacg gccttcgtga gtttggcacc gacggcggtg
     2941 ccgcagcatg tcacctcgat cggcggcgtc agcggtgtta tcagcgtggg caacgggctg
     3001 caggtgacga gcggccagct ggggctcacc agcgccatta cccaaaaact cggacaatca
     3061 tcgggtagtg gtgtcagcag cttgacgtcc ggcacctcca atctcgtgat taccaatacc
     3121 ggcaacggta attacacatt gagtgacaat tccgtggtaa acagcggtag cacggggcaa
     3181 attgctctca tcaccggcgc tcagaccatt ggcggctcca tcttgagcca gagcggctcg
     3241 agcctgacgg cggggggcac attgacggtc agtggcggcc tgcttaccaa cagcctccag
     3301 caaacggcgg cgggcaataa cgttgctatc tcggccggct ctgacagcat cgtcttcacc
     3361 gacggcagcc ggacatttgc gttcccagcc ggcggcgggg ccaatcaaac catttgtacg
     3421 acaggggtga cttgcgcttc gggcggcggg attgcggtgc ttttgcagcc gggggcggct
     3481 caagccgata ccggtgccgg tagctcaatt ttcgtcaata acaccggtgg cggcaactta
     3541 ctcgaactgc aaggggccgg cagcgaccgc ttcgtggtga ctaatggcgg caatgtgacg
     3601 gccggtggca cgctgacact tggtagcgca ctgaccgtag cgaacggcgg taccgggggc
     3661 gtcagcctga ccgcgaacgg cgtgctttac ggcaatggag ctggtgctgt ccaagctacg
     3721 ggcgccgcag ccaacagcat cttggcgacc aacggttcga atgtaccagg attgacgcaa
     3781 acgttgccaa ttgccgtgca aagcaatatc actactaccg gcatcttggc gaccggctcc
     3841 atcgccagtg gcttcggcgc gatcagtact tctaacaaca tcactacctc cgcagcgctg
     3901 cagggcggga cgcttagcgt ggcgagtggc aacttcacca taaacagttc tggaaacgtg
     3961 gttactaccg ggacagccac tatccaaggc gctggagggc ttagcattgg tgtagctgct
     4021 acaacggctg gcagtctcgt ttttgctaat gctagcaatg gacacttgac caccttgcaa
     4081 ggactggccc cagccggcca ggaccagact atcaccatcc ccgcctcaag tgctgcaaca
     4141 gatactgttt gtctcttgac gctggccaac tgtgtcggag cgggcggcgg cgtaaccgcc
     4201 agcggcggca cgcagaactt cgtgccgaag ttcaccaacg ctggcggcac ccagcttggc
     4261 aactcactta tttttgacaa cggcaccagt gtcggagtga acaatagtac gccgggagct
     4321 agtttcaaac ttgacgtcgg cggcaacgtc aacatttcgg gtaatacggt cgtggggacc
     4381 gggctgaccg tgaccgctgg gggagcaaca gttagtgccg ggggcattgc ggttacgggg
     4441 aacagcacca tcagcggcac tttaacttcg ctgacggggc ttacta
//
LOCUS       DATNGP010000112         8988 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_4463571, whole genome shotgun sequence.
ACCESSION   DATNGP010000112 DATNGP010000000
VERSION     DATNGP010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 8988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8988
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4463571"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="VN778_02800"
     CDS             <1..183
                     /locus_tag="VN778_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012918210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HXR49931.1"
                     /translation="ENITYTYLVNAHWYPEAHYTFVNLFDPELGIDWPIPKDQAIFSE
                     KDAGHPLLKDVTPMEV"
     gene            189..1046
                     /locus_tag="VN778_02805"
     CDS             189..1046
                     /locus_tag="VN778_02805"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HXR49932.1"
                     /translation="MDNSSIFIVGANGQLGRALQTQYPGAHSADINELDITDASSVAN
                     FDWSQVKTILNAAAYTNVDGAETADGRVAAWKVNASAVGNLVRAAQQHDLTLVHISTD
                     YVFDGTGHPHTEDEPFSPLSVYGQSKAAGDIAVSLLPKHYILRTSWVIGEGKNFVRTM
                     LELGKKGVSPSVVADQIGRLTFTSELVRAIDHLLTSTPVYGTYNVSNSGDEASWADIT
                     RAIFQAASFDLQVTDTTTAEYYAGKDGIAPRPLQSGFDLSKIQSTGFTSTDWREDLSK
                     YVAKELSQT"
     gene            1043..1903
                     /gene="rfbA"
                     /locus_tag="VN778_02810"
     CDS             1043..1903
                     /gene="rfbA"
                     /locus_tag="VN778_02810"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018190040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HXR49933.1"
                     /translation="MKGIILAGGSGTRLYPITKGISKQIMPIYDKPMVYYPLSTLMQA
                     GIQDILIITTPDDQAQFQRLLGDGSDLGINLQYAAQPKPEGLAQAFIIGEEFVGQDKV
                     ALILGDNIFYGEGFGASLRDCVDPNGGIVFAYEVSDPERYGVVEFDAENRAVSIEEKP
                     AQPKSNYAVVGLYFYDNNVIEIAKNIQPSARGELEITTVNEEYLKRGKLKVQTMDRGS
                     AWLDTGTFESMNDATEYIRVIEKRTGIKIGCIEEVAYRQGFINKERLQQIAEPLVKSG
                     YGKYLLSLTS"
     gene            1909..2856
                     /locus_tag="VN778_02815"
     CDS             1909..2856
                     /locus_tag="VN778_02815"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HXR49934.1"
                     /translation="MYKKLRVAVVIPAYNEEKLIAKTVTTLPDSIDYIIVVNDASTDN
                     TLQVLKHLANEDDRLHVLDNERNKGVGGSLIRGFKFALSDTDVEAIGIAAGDAQCDPA
                     YIEPMLDELIKHDLDYIKANRFFHRDALKAMPAYRQIGNIFISLLTKFSTGYYSISDT
                     QNSYGFFTRSILEQVNFDFVKERYDYENTMLIALSIAGAKLKDHPVPAIYGDEVSTIK
                     FLPTALRALRAVWVGFWQRIYYKYVLYSFHPIALFLFSGIFLGTLGTIYGLFIGYRKV
                     FEHLSPSIGTVMLISLPLFLGFQLVLTAIMMDMGNEGRS"
     gene            2857..3225
                     /locus_tag="VN778_02820"
     CDS             2857..3225
                     /locus_tag="VN778_02820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49935.1"
                     /translation="MLTIIAWMVVYAVVVAASIIFIGKPIEGNVHFGSLLRLLLDWRF
                     LLGGFLALGARFIFVIINNLASKQAALADAHLTIAALATQGSIIAIILANAIFLGERL
                     QPVQLLGAGIIICGVFLIFR"
     gene            3321..4496
                     /locus_tag="VN778_02825"
     CDS             3321..4496
                     /locus_tag="VN778_02825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49936.1"
                     /translation="MALENSKPSLPWLAEESFWQQNQEALRDLFHTVNRQYPEHGAEA
                     LIQETEKDEPSPEAMRWMWQRFMGSQHGTMAGADDKISLTRQSEYLYWPDFSRVRYGN
                     HYLTIPSKLDAEREYTKAFVLSGTQQENVDRLLTVIGHDPSNESVHEIIVMSGQRTRG
                     WEAPGEKSVKDLVTVTAQMSGADMEKLRDDSPFVQSELAKRGTGWEGSFPSEYEMCRL
                     AVEAVFQKQINWSDYPVDVTEGRDEEPLIYGGHGELSIPARAEVIATYSLLDSRKVHV
                     VNAKAVQRNGSDVPRATSDSQTREAVDLLLPEQSREHIVIASSVPHTRAAFDALIRII
                     DMRKSNIERADIVTGKWLPWMELLAGFGEIPATHKADKRLRAVLAGKDPDSKKLSDL"
     gene            complement(4508..5260)
                     /locus_tag="VN778_02830"
     CDS             complement(4508..5260)
                     /locus_tag="VN778_02830"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXR49937.1"
                     /translation="MAKREPRTMAEDELLEKERQTLAQVSGWYKNTEWGFYTKIVWYS
                     YVSIKPFFHGKKCLEMGPADGEMTKFLLHDFDKLVVVDAAEEYVKAAEKLSENINGYT
                     SLFEEFEPKEKFDTIIMSHVLEHVNDPQLVLNRAKQWLNKDGRIIMVVPNADSLHRRL
                     GVKLGMLERENQLNDQDIEIGHRRVYTREELDQDIRSTGLATIAKGGIFLKLLSNTQM
                     LTFKDESLIEGMFELGKDFPQYCSEIYAVCAR"
     gene            complement(5215..6213)
                     /locus_tag="VN778_02835"
     CDS             complement(5215..6213)
                     /locus_tag="VN778_02835"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HXR49938.1"
                     /translation="MIKGKKVLVTGGAGFIGSHSVERLLKEGATVTVLDNFRTGLRKN
                     LENVKNDINLLEGSILEAGDLANAVKGQDLILHLAAQLEIMRAIDDPVEDLITNTVGT
                     LNVFKAAVEQGIPKVVMASSAGVYGQAVETPQREDSHPTEPNWAYGVSKLATEKYAAI
                     FQEANGLKITGLRYGIVYGTREWYGRVLTIFLKCALEDRPLVIFGDGKQVRDFINVKD
                     VVNMNMLCLDNAKADNEVFNVSTAKGTDINTLADIVKQASGKSIEIIHEELKEGEKSK
                     YSERIRLPQELKELLQSRDKAKELLSWEPDVDLAAGIKEEYQWLSENPERWLKMNY"
     gene            complement(6210..6818)
                     /locus_tag="VN778_02840"
     CDS             complement(6210..6818)
                     /locus_tag="VN778_02840"
                     /inference="COORDINATES: protein motif:HMM:TIGR01509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HXR49939.1"
                     /translation="MGINPSIKAVIFDLDGTLVDLPIDWEAVKYQAGKRADQSFGDRV
                     EELIDANNSEILQRIADHEMASLDKAHISSEIAKVLSELDHTYRLAILTRNSRQVAER
                     FLELCGINSQRYLIVGREDISRLKPHPEGLEIILKQLHINPSEAVMVGDTFHDTELAH
                     QNGLYSVLVGNKTEAKHSDKAFQADNLSDVVAIIHNINKEEA"
     gene            complement(6797..7861)
                     /locus_tag="VN778_02845"
     CDS             complement(6797..7861)
                     /locus_tag="VN778_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018193490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49940.1"
                     /translation="MVRVLLTGAGGSAAYNFCDALKLVKNDLYIVGTDAKKYHLELMN
                     IDKKYLVPPVTDPEYVPAINKIIEQEQIDFLHPQPDVEVAFWAKNTDKIKAKTFLPAN
                     KEVELCHDKMKFNTVLKEKGVAVPDAFHITDKANLEASLKKLLKDHPKVWIRAIRGAG
                     SRASLPVDSFYQADAWIDYWNHFRGLNYPDFMISEFLPGHEYAWQSLWYEGKLISAQA
                     RERIEYIFGNLTPSGQTSSPSVAKTVNRDDINEIGQAAVRATSDKPHGVYCVDMKEGV
                     DGVPKVTEINIGRFFTTSNFFAHAGLNMPAMYLELGMTGKLEKKPQQLNALPDDLYWV
                     RMIDMGFKLVKEGEWESTQA"
     gene            complement(7874..8848)
                     /locus_tag="VN778_02850"
     CDS             complement(7874..8848)
                     /locus_tag="VN778_02850"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HXR49941.1"
                     /translation="MPSRKTRVAVVGAGNMGRNHLRNYFLLPEFELVGLADIDPNTKT
                     VAEEYKAKHYTDFELMMDELKPNAVSIVVPTPFHARVATAALSRGIHCLVEKPIASTV
                     KEADELIKLAKKQKVIFTVGHIEHYNPLIRKLKQMVDEGSVGKVTSVVCKRVGGFPAV
                     EPKTDVIIDLAVHDIDIVSHLLGQQPKRIYSHGSRTHHSREIDSAEILMDYGDASGFV
                     QANWLTPVKIRTIAITGSEGYLEGNYITQELEYYKHNMQKLESGFSNFVIKVGEPQKR
                     IIKVDFEEPLAVELKAFYDKIQGDNTVHLVDPGAAREALRLALEAVGK"
     gene            complement(8841..8987)
                     /locus_tag="VN778_02855"
     CDS             complement(8841..8987)
                     /locus_tag="VN778_02855"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HXR49942.1"
                     /translation="MANHGFKPGDFPEAERAAEQALSLPVHPKVSEADAKHIIDSLKE
                     LASA"
BASE COUNT         2255 a         2228 c         2069 g         2436 t
ORIGIN      
        1 gaaaacatta catacaccta cctggtcaac gcccactggt atccagaggc tcactacacc
       61 ttcgttaacc tgttcgatcc cgagcttggc attgactggc ccatccccaa agaccaagcg
      121 attttttcag aaaaggatgc cggacatcca ctacttaaag atgtaacccc gatggaggtt
      181 tagtagacat ggataactcc tcaattttta ttgtcggcgc caacggccag ttgggtaggg
      241 cgctgcaaac ccagtatccc ggcgcccata gtgccgacat caatgagctg gatattacag
      301 acgcctcgtc ggttgctaat tttgattgga gccaggtcaa gactatccta aatgctgctg
      361 cttacacgaa cgttgacggt gccgagactg ccgacggacg ggtagccgct tggaaagtca
      421 acgcctcagc ggttggcaat cttgtgcgtg ccgcccagca gcacgatctg accttagtgc
      481 atatttctac agactacgtc tttgacggca cggggcatcc ccacaccgag gacgagccgt
      541 ttagcccttt gagtgtctac ggccaaagca aagcagccgg tgacatcgcg gtcagcctgc
      601 tacctaagca ctacattctc cgcaccagct gggtcatcgg cgaaggcaag aactttgtcc
      661 gtactatgct agagctcggc aagaagggcg tcagtcctag tgtggtagcc gaccagatcg
      721 gccgcctaac cttcacgagc gagctggttc gcgccatcga ccacctgctc acttcaacac
      781 ctgtctacgg cacgtataac gtcagtaaca gtggcgatga agccagctgg gccgacatca
      841 cgcgcgccat tttccaagca gcctctttcg acctccaagt aaccgacacg acaaccgctg
      901 aatactacgc cggcaaagat ggcatcgccc cacgcccttt gcaaagcggc tttgacctct
      961 ctaaaattca atccaccggc ttcacctcca ccgattggcg cgaagactta agcaagtatg
     1021 tagcaaagga gctatcacag acatgaaagg cattattcta gcaggcggct cgggcacccg
     1081 tttatacccc attaccaaag gcatctccaa gcaaatcatg ccgatctatg acaagccgat
     1141 ggtttattac ccgctcagca cgctcatgca ggccggcatc caggacattc tgataattac
     1201 gacgcctgat gaccaggcac aattccagcg cttgttgggc gacggttctg atctaggtat
     1261 taatttacag tatgcggccc agccaaagcc ggaaggtctg gcccaagcct ttattattgg
     1321 cgaggaattt gtcggccaag acaaagtagc tctgattttg ggcgacaata tcttctatgg
     1381 cgagggcttc ggtgccagct taagagactg cgtcgatcct aacggtggca tcgtctttgc
     1441 ttatgaagta tccgaccctg agcgatatgg cgtcgtcgaa ttcgatgcgg aaaatcgcgc
     1501 cgtatccatc gaggaaaagc cggctcaacc aaaatcaaat tacgctgttg ttggtttgta
     1561 tttttatgac aataacgtca tcgaaatcgc taagaacatc cagcccagtg cccgcggcga
     1621 actagaaatc accaccgtta acgaagaata tttgaagcgt ggcaagctca aagtccagac
     1681 catggaccgc ggttccgcct ggctcgacac cggcaccttc gaatccatga atgacgccac
     1741 cgaatacatc cgcgtcatcg aaaagcgcac cggcatcaag atcggctgca tcgaagaagt
     1801 cgcttaccgg caaggtttca ttaataagga gcggctccag caaatcgctg agcccttggt
     1861 caagagtggg tatggcaaat atttgctaag cctaactagc taatgtatat gtataagaaa
     1921 ctgagagtcg ctgtcgtcat acccgcttac aacgaggaaa agctcattgc taagaccgtg
     1981 acgacactac cggattctat cgattatatt atagtcgtca atgatgccag cacggacaat
     2041 acactccaag ttcttaagca cctggcaaat gaagacgacc ggcttcatgt cctagataat
     2101 gaacggaaca agggggttgg cggcagcctt attcgcggct ttaagtttgc gctttctgac
     2161 actgatgtcg aagctattgg tatcgcggcc ggcgatgcgc agtgtgatcc ggcctatatt
     2221 gagccaatgt tggatgaact tatcaaacat gatttggact acataaaagc taaccgcttc
     2281 tttcatcgcg atgcgctcaa ggctatgccc gcctatcggc agatcggcaa tattttcatc
     2341 tccttgttaa ccaagttttc gacggggtat tattcaattt ccgataccca gaacagttac
     2401 ggctttttca ctcgtagcat cttggagcag gtgaattttg atttcgtaaa agagcgctac
     2461 gattacgaga ataccatgct gatagcactt tccattgctg gcgcaaaatt gaaggatcat
     2521 cctgtaccgg ctatctacgg cgacgaagtc tcaactataa agtttctacc gacagccctg
     2581 cgagcactca gggccgtgtg ggttggcttt tggcagcgta tctactacaa gtacgtattg
     2641 tacagttttc atcccatcgc tctgtttctt ttcagcggca tttttctcgg tacacttggc
     2701 actatttatg gcctatttat tggctatcga aaggtctttg agcatctttc gccttcgata
     2761 ggtactgtta tgttaatctc gttaccgctt ttccttggct ttcagcttgt gctcacagct
     2821 attatgatgg atatgggcaa cgaggggagg tcttaaatgt taacaattat agcgtggatg
     2881 gtggtatatg ccgtggtcgt cgcggcctca atcattttta ttggtaagcc aatcgagggg
     2941 aatgttcact ttggatcttt gttgcgacta cttttggatt ggcgttttct gctcggaggc
     3001 tttctggcac tgggggctag atttattttt gtcatcatca ataatctggc gtctaagcaa
     3061 gctgcgctgg ccgatgccca tctcaccatt gcagctttag cgacgcaagg tagtattatc
     3121 gctatcatct tagcgaacgc catttttcta ggcgaacgct tacagccggt gcagctgctc
     3181 ggcgccggca tcatcatctg tggagtcttc ttgatttttc gttaagctac ggcagcaaga
     3241 tcaagttgtt ttgagctttc aaggtacagg ttatttgaat attggacatt tactaataaa
     3301 tatgctatta taactaagta atggctcttg aaaactcaaa accatctcta ccctggcttg
     3361 cggaagaatc tttctggcaa cagaaccaag aagctttgcg tgacctattt catacggtca
     3421 accgacagta ccctgagcat ggagcggaag cactgataca ggaaacggaa aaggacgagc
     3481 cttcgccaga agccatgcgc tggatgtggc agcgcttcat gggcagtcaa cacggtacta
     3541 tggcaggagc agatgacaaa atctcactta ctcgtcagtc agaatatttg tattggccgg
     3601 attttagtcg tgtccgctac ggtaatcatt atctaactat tcctagtaag ttggatgctg
     3661 agcgagaata cactaaggca tttgtcttgt ccgggacaca acaagaaaac gttgatcggt
     3721 tacttacggt tataggtcat gatcctagta atgaaagcgt tcacgaaatt atcgtcatga
     3781 gcgggcaacg aacaaggggc tgggaagctc ctggcgaaaa gtcagttaaa gacctggtca
     3841 ctgtgacagc ccaaatgagt ggagccgata tggaaaagct acgcgatgac tcgcccttcg
     3901 tccagtcgga actagccaaa cgtggcaccg ggtgggaggg ctccttcccc agcgaatatg
     3961 aaatgtgtcg tttggccgta gaagctgtct tccagaagca gataaattgg tcggactatc
     4021 cggtggatgt gaccgaaggc cgcgatgaag aacccttaat atatggcggg catggcgaac
     4081 taagcatacc cgcacgtgca gaagttatag ctacttatag cttacttgac agtcggaagg
     4141 tgcacgtcgt gaacgctaag gccgtacaaa gaaatggtag tgacgtacca cgggcaacgt
     4201 cggattcaca gacccgggaa gctgttgacc tattacttcc tgaacaaagt cgggaacaca
     4261 tagtaattgc ttcaagtgtg ccacatacca gagcggcttt cgacgcacta atacgtatta
     4321 ttgatatgcg caagtccaat atcgaacgcg ccgatatcgt cacaggcaaa tggttgccat
     4381 ggatggagct gctcgctggc tttggtgaaa ttcctgccac tcataaggcg gacaagcgtt
     4441 tacgggcagt ccttgccggt aaagaccctg actccaagaa gctttcggac ctttagagcc
     4501 gaagttgcta ccgtgcacag acggcgtaaa tttcagaaca atactgcgga aagtctttgc
     4561 cgagctcaaa catgccctcg atgagcgact catccttgaa ggtcaacatc tgagtattag
     4621 aaagtaactt taaaaagatg ccgcccttgg cgatagtagc taaaccggtt gatcgtatgt
     4681 cctgatccag ctcttctctg gtatatacac gccgatgacc gatctcgatg tcctggtcat
     4741 tgagctgatt ctcacgctcc agcataccca acttaacgcc caggcgcctg tgcagagaat
     4801 cggcgttcgg tacaaccatg atgattcttc catctttgtt cagccactgc tttgctcgat
     4861 ttaggaccag ctgcggatcg ttcacgtgct ccaagacatg tgacataatg atggtgtcaa
     4921 atttttcttt cggctcaaat tcctcaaaca gactggtgta gccattaata ttctcactca
     4981 gtttttccgc agctttaaca tattcctccg cagcatcgac aactacgagt ttgtcaaaat
     5041 catgcaatag aaattttgtc atctcgccgt ctgccggacc catttccaag catttcttac
     5101 cgtggaaaaa cggcttaatg gaaacatatg aataccaaac tatttttgtg tagaaacccc
     5161 attcggtgtt tttgtaccaa ccagaaacct gggccagggt ttgccgttct ttttctagta
     5221 attcatcttc agccatcgtt cggggttctc gcttagccat tggtattcct ccttgatgcc
     5281 ggcagctaga tcgacgtccg gttcccagct tagtagctct ttcgctttat cacgggactg
     5341 caatagttct ttgagctcct gcggtaaacg aatcctttcg gaatatttgg acttttcgcc
     5401 ttctttaagc tcttcatgaa taatttcgat tgatttaccg ctagcctgct taactatgtc
     5461 agcaagagtg ttaatgtctg ttcctttagc agtagagaca ttaaatactt cgttatcggc
     5521 cttggcattg tccaggcaaa gcatgttcat atttaccaca tctttgacat tgataaagtc
     5581 tcggacttgc ttaccgtcgc caaaaatgac gagcggcctg tcttcgaggg cacatttcag
     5641 gaaaattgtc agaacgcggc cataccattc ccgtgttcca taaacgatac catagcgtag
     5701 gccggtaatt ttgaggccgt tggcttcctg gaagatggca gcgtacttct cggtcgccag
     5761 tttgctaact ccatacgccc aattcggctc ggtcgggtga ctgtcttcac gctgcggcgt
     5821 ttcgacagct tgaccgtaaa ccccagcaga agaagccatg acaacttttg gaataccctg
     5881 ctcgacagcc gctttgaata cattcagggt gccaacagtg ttggttataa ggtcttcgac
     5941 tggatcatca atggcccgca taatttccag ctgggctgct aaatgcaata ttaggtcttg
     6001 gcctttgact gcattagcta ggtctccggc ttcaagaata ctgccctcta gaagattgat
     6061 atcgttcttt acgttttcaa ggtttttccg caaccctgtt cggaaattgt ctaatacagt
     6121 gacggtcgcg ccttctttca agagacgttc cacgctgtgt gaaccaatga atccggcacc
     6181 gcccgttact aatacctttt taccttttat catgcttcct ccttatttat attatgaatg
     6241 attgctacaa catcagacaa attatctgcc tgaaaagctt tatcgctatg cttggcttcg
     6301 gtcttgttac cgaccagtac tgagtatagg ccattttggt gtgcaagctc tgtgtcatga
     6361 aaggtatcgc cgaccatcac ggcctccgac ggattgatgt gaagttgttt gaggataatc
     6421 tccaggcctt cggggtgtgg tttcagacga gagatatctt cacgcccgac tatgagataa
     6481 cgctgcgagt ttatgccaca caactcgaga aagcgttccg caacctgtcg agaattgcgc
     6541 gttaagattg ccagcctgta cgtatggtca agctcactaa gaaccttagc tatctccgag
     6601 gatatatgcg ccttgtctag cgaagccatt tcgtgatcag ctatcctttg taggatctcg
     6661 ctattgtttg catcgatcag ctcctcgaca cggtcaccga aactttgatc tgcccgctta
     6721 ccagcctggt atttcacagc ttcccagtcg atcggcaagt cgaccagggt cccgtccagg
     6781 tcaaaaataa ccgcttttat gcttgggttg attcccattc accctcttta acaagcttga
     6841 agcccatatc aatcatgcgt acccaatata aatcatcagg taaagcattt agctgctgtg
     6901 gtttcttttc gagctttcca gtcatgccaa gttcaagata catcgctggc atgtttagtc
     6961 cagcatgggc aaagaagttg ctggtagtaa agaaacggcc aatgttgatc tcggtgactt
     7021 ttggaacgcc atcgacgcct tctttcatgt caacacaata cacaccatgc ggcttgtcgc
     7081 tggtggcacg cacagcggcc tgcccaattt cattgatatc gtctcggtta accgttttag
     7141 caaccgatgg ggatgaggtt tgaccactgg gcgttaaatt accaaagatg tattcaatac
     7201 gctctcgagc ttgagctgaa atcagtttac cctcatacca caagctctgc caagcgtact
     7261 catgaccagg taagaactca ctgatcatga agtccgggta attgagacct cgaaaatgat
     7321 tccaataatc tatccaagca tcagcttgat aaaagctgtc aaccggtagg ctagcacgtg
     7381 agccggcacc acgaatagcc cggatccaga ccttagggtg gtctttgagg agtttcttaa
     7441 gtgaagcttc caggttggct ttgtcggtga tatggaatgc atcaggtact gctacgcctt
     7501 tttcttttag tacagtgttg aacttcattt tgtcatgaca caactcaact tctttattgg
     7561 ctggaagaaa agttttagcc ttgatcttgt cggtgttttt agcccaaaag gcaacttcga
     7621 catcgggttg gggatgcaga aagtcaattt gctcttgttc aataatttta ttgatagccg
     7681 gcacgtactc agggtcagtt acgggcggaa ctaaatactt cttgtcaata ttcattagtt
     7741 ccaggtgata tttcttggca tcagtaccaa caatataaag atcattcttg acaagtttta
     7801 gcgcatcaca aaaattatac gcagccgatc ctccagctcc tgtcaataat acacgtacca
     7861 tgttcctcct tatttacttt ccgactgctt ctagcgccaa gcgcagagct tctctggctg
     7921 cccctggatc tactaagtgc accgtattat caccttgtat tttatcgtaa aacgccttta
     7981 gctctacggc taatggctcc tcgaaatcca ctttaataat ccttttttga ggctctccta
     8041 ctttaataac gaagttggaa aaaccgcttt caagcttctg catattatgc ttatagtact
     8101 cgagctcttg cgtaatgtaa ttgccttcta agtaaccttc agaaccggta atagcgatag
     8161 ttctaatttt taccggagtt agccaattag cctgaacaaa gcccgaagca tcgccgtaat
     8221 ccatcagtat ttccgctgag tcgatttccc ttgagtgatg agtacgtgag ccatgactgt
     8281 aaatcctttt gggctgctgg ccaagcagat gacttacaat gtcgatatcg tgaacggcca
     8341 aatcaataat aacgtcggtc ttgggctcaa ctgccgggaa accacccacg cgcttacaaa
     8401 ccacagaagt tactttgcca acacttcctt catcgaccat ttgcttgagc tttctaatga
     8461 gcggattgta gtgctcgatg tgcccaacag tgaagatgac tttttgcttt ttggcaagtt
     8521 taataagttc gtcagcttct tttacggtac tcgctatggg cttttcgacc agacagtgaa
     8581 tccctcgact cagggccgca gtagctaccc gggcatggaa gggagtcggt acaacgattg
     8641 atacggcatt aggctttaac tcatccatca tgagttcaaa atcggtgtaa tgtttggctt
     8701 tgtactcttc ggctacagtt ttggtgttag ggtcaatgtc tgccaggccg acaagttcaa
     8761 attcaggtag aagaaaataa tttctgaggt ggtttcggcc catgttacct gccccaacaa
     8821 ctgcaacacg agttttgcgg ctaggcacta gcgagctcct tgagtgaatc aataatatgc
     8881 ttggcgtccg cttcactcac cttcgggtgt actggcaatg acaaagcttg ttcagcggcg
     8941 cgttcagcct caggaaaatc tcctggctta aaaccatggt tcgctatg
//
LOCUS       DATNGP010000113         2169 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_1355726, whole genome shotgun sequence.
ACCESSION   DATNGP010000113 DATNGP010000000
VERSION     DATNGP010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2169
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1355726"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1265
                     /locus_tag="VN778_02860"
     CDS             <1..1265
                     /locus_tag="VN778_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013726699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HXR49943.1"
                     /translation="TVGDKIFYVDKTVDDIAVEVALQYNDAFNETVKAFANNVLTPEG
                     GTHLVGFRTALTRVINDYARKNGLLKEKEDNLSGDDVREGLTAVILVKIPDPQFEGQT
                     KSKLGNPEVRGAVDKVFSEYLNYFFEENPTIAKGIVGKATLAARARKAARAARDNIIR
                     KGVLDGASLPGKLADCSSKNPAESEIYLVEGDSAGGSAKTGRDHKTQAILPLRGKVLN
                     VERARLDKMLANAELLSLIKALGVGIEDSFDVGGLRYGRIIIMTDADVDGSHIATLLM
                     TFFFRYMKQLLDDGHVYLARPPLFELVKPGRKSSEFIYDEADLDTVIDAAIERRKKEG
                     LKVNPDEERFKQAGFIEQKRYKGLGEMDATQLYETTMNPEKRVLVQVRVDDAEKADVI
                     FNKLMGSEVELRKNFIQTNAKFVKDLDI"
     gene            1354..>2169
                     /locus_tag="VN778_02865"
     CDS             1354..>2169
                     /locus_tag="VN778_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HXR49944.1"
                     /translation="MVEDVMEDNYLRYSMSVIIDRALPDVRDGLKPVHRRILYTMNRD
                     GLRSSARHRKSATVVGAVMGDYHPHGDASIYDSLVRMAQDWSMRYMLVNGQGNFGSMD
                     GDPPAAMRYTEAKLQRVADEILEDIDKETVDFRDNYDGRLQEPTVLPAKLPNLLLNGQ
                     IGIAVGMATSIPPHNLSELVDATVYQIDHEDAALDDLLEFVKGPDFPTGGVIYGKDSI
                     RSAYAVGRGGVVVRGVAEIVENNRGRHQIIITEIPYALNKESLVIKIADLVKDK"
BASE COUNT          540 a          534 c          613 g          482 t
ORIGIN      
        1 agaccgtggg cgacaagatc ttctacgttg acaagacggt tgacgacata gcggtagagg
       61 tggctctgca gtataacgac gcgttcaacg agacggtcaa agcctttgcc aacaacgtac
      121 ttacccctga aggcggcacg catttggttg gtttccgaac cgcactgacc cgcgtaatta
      181 acgactatgc gcgtaaaaat ggcctactga aggaaaaaga agacaacctg agtggtgatg
      241 atgtccgtga gggtttaaca gcagttatcc tcgtcaaaat tcctgatccg cagtttgaag
      301 gccagaccaa gagcaagctc ggcaaccctg aagtccgcgg tgctgtcgac aaggtgttca
      361 gcgagtatct aaactacttc tttgaagaga accctacgat cgccaagggg atagtaggca
      421 aggcaaccct tgctgctcga gcacgcaaag ctgctcgcgc tgcccgcgac aatattattc
      481 ggaaaggcgt gctggacggt gccagtcttc cgggcaaatt ggctgactgc tcgagcaaga
      541 accctgctga gtctgaaatc tacttggtag agggcgactc ggctggtggc tcagccaaaa
      601 ctggccggga ccataagacc caagctatct tgccgcttcg aggtaaggtg ctgaatgtcg
      661 agcgggcgcg actcgataaa atgcttgcca atgccgagct gctgagcctc attaaggcgc
      721 tgggcgtcgg catcgaagat tcgtttgatg tcggtggtct gcgctacggc cgcatcatta
      781 tcatgaccga tgccgatgtt gacggttcgc atattgcaac gctcctgatg acattcttct
      841 tccgttatat gaagcagcta ctcgatgatg gtcacgtcta cttggcgcgg ccgccgctct
      901 ttgagctggt gaaaccgggt cgcaagtcga gtgaatttat ttatgacgag gctgacctag
      961 ataccgtcat cgatgcagct attgaacggc gcaaaaaaga gggattgaag gtcaatcccg
     1021 acgaagaacg tttcaaacaa gctggcttca tcgaacaaaa gcgttacaag ggtctcggcg
     1081 aaatggatgc tacgcagttg tacgagacaa caatgaatcc cgaaaagcgg gtgctcgtac
     1141 aggttcgtgt tgacgatgcc gagaaagcgg atgtaatttt taataagctg atgggtagcg
     1201 aagtggagct acgcaagaac ttcattcaga ccaatgcgaa gtttgtgaaa gacttggata
     1261 tttaatatgg atgaccgaaa tttagccgca ctgccagagc ccgaagaggg agaagttcta
     1321 gacgccagcc attcgcgctt ggttgagcgc cgtatggtcg aggatgtcat ggaggacaac
     1381 tacctacgct actccatgag cgtcattatt gatcgtgcgt tgccagacgt ccgcgacggg
     1441 ctcaaaccgg ttcaccgacg tattttgtac actatgaacc gcgatggctt gcgcagttca
     1501 gcgcgccacc gtaagtcggc taccgtcgtc ggcgcggtga tgggtgatta ccacccgcat
     1561 ggcgacgcct cgatttacga ctcgctggtc cgtatggccc aagactggtc gatgcgctac
     1621 atgctggtca atggtcaggg taactttggt tcgatggacg gcgatccgcc ggccgcaatg
     1681 cgctatacgg aagctaagct gcaacgggta gccgatgaga tcctggaaga catcgacaaa
     1741 gaaactgtcg atttccgcga taactacgac gggcgcttgc aagagccaac cgttttacca
     1801 gctaagttac caaacttgct cttgaacggt cagatcggta ttgccgtcgg tatggcgacg
     1861 agcattcctc cccataacct cagtgagctt gtcgatgcaa cggtgtatca gatcgatcac
     1921 gaagacgctg cactcgatga tctgcttgaa tttgttaaag ggcctgactt cccaaccggc
     1981 ggtgtcattt acggcaaaga ctcaattcga tcggcgtacg ctgttggccg aggcggcgtg
     2041 gtcgtgcgcg gggtagccga gatagtagag aacaacagag gccgccacca gattattatt
     2101 actgagatcc cctatgcgct gaataaggag agtctggtta tcaagattgc cgacctggtg
     2161 aaggacaag
//
LOCUS       DATNGP010000114         6008 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_3188406, whole genome shotgun sequence.
ACCESSION   DATNGP010000114 DATNGP010000000
VERSION     DATNGP010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6008
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3188406"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="VN778_02870"
     CDS             complement(<1..185)
                     /locus_tag="VN778_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012582458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HXR49945.1"
                     /translation="MSNTNITVYSATWCAFCHAAKNYFDQKGVKYTDKDVETDPSAGL
                     EAVDKSGQRGIPVIDIDG"
     gene            266..559
                     /locus_tag="VN778_02875"
     CDS             266..559
                     /locus_tag="VN778_02875"
                     /inference="COORDINATES: protein motif:HMM:NF021073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HXR49946.1"
                     /translation="MKQTTASPTPEERKSNAPTDSRSPGAAFVVMALDMSWRLALIVL
                     IPILGGFQVDKKLNTSPLFTLVGFVLAMAGTGYVLWWASKKADSLAMPKGGAK"
     gene            556..1431
                     /gene="atpB"
                     /locus_tag="VN778_02880"
     CDS             556..1431
                     /gene="atpB"
                     /locus_tag="VN778_02880"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HXR49947.1"
                     /translation="MIAGLLTRFAIEGSPTVHVAPGTDFTIAGIPITNAMFYGWICTI
                     SIIIFLIWAARKVTVKPQGGVVQYVEAGVEFVTNFVEGSFTDPAVGRKYVPFFVTLFF
                     FVLFNNWSELIPVVGDGIQLHGHPLFRPVTADLNMTLAIGLVTMLVVYAASIKETGGP
                     IKYLRHFFIGNLLNPLYLVIGIIEIITDLTRVFSLSLRLFLNITIGEIVIIVFAFLGG
                     ALLPHSVLSPVTAVPFWLLELGVGVLQAYIFAILGTNYLAIAVNSAQDHTHDDLTEGG
                     PPETIEVAAREAVGG"
     gene            1514..1741
                     /locus_tag="VN778_02885"
     CDS             1514..1741
                     /locus_tag="VN778_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017935507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HXR49948.1"
                     /translation="MVFADAVNTTIIGKGLMIGGGFIGPTIGIGLIGANYLQAVGRNP
                     EASRFFGQAIIFVGLVEVYGLLAFASTFFVK"
     gene            1764..2297
                     /gene="atpF"
                     /locus_tag="VN778_02890"
     CDS             1764..2297
                     /gene="atpF"
                     /locus_tag="VN778_02890"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HXR49949.1"
                     /translation="MIQTLTFFGDSSSGIGALGVDGKAFVIQLITFLLAYWVLQRYAF
                     GPILKVMRERRETIESGVKLGEQMQKDKAALEAQVEETLHKAREQADGIITGAQDTAR
                     QTVREAEEKAREKAAGIVDAAEARIEQDTARARRQLEKEIVGLITDATEAIIEEKVDA
                     KKDAELIDRALKERQAA"
     gene            2294..2698
                     /locus_tag="VN778_02895"
     CDS             2294..2698
                     /locus_tag="VN778_02895"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HXR49950.1"
                     /translation="MKQSRTKISRVIADQTLAGGDPKQLSRELAAYLLSERRVSELSS
                     ILRDVQADWAEAGHVEVIAASAHPLSAETTSEIECQVKRLLPDAKQIVVVETRDPAVI
                     GGVRLNLANQQLDLSIEAKLNKFKQLTTAGKD"
     gene            2703..4256
                     /gene="atpA"
                     /locus_tag="VN778_02900"
     CDS             2703..4256
                     /gene="atpA"
                     /locus_tag="VN778_02900"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015642046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HXR49951.1"
                     /translation="MAELTLTELSEDIRAAIAELKERPEIENVGVVTRVGDGIAWIYG
                     LSAAGYSEMLEIEGVDSQQVTAFALNLAEDEIGAVILGDDTQVKAGAKVRLSGKVLEV
                     PVGPELVGRIVDPLGNPLDGGEPIKAKQTSRVERQAPGVLERKHVHEPMMTGLMAIDS
                     IVPIGRGQRELIIGDRQTGKTAIAVDTMVNQARQKTGVINVYVAVGQKLSKVAALQER
                     LKREGVMDQTIIVATSPADPAALLYLAPYAGCAMGEYFRDNKQHALIIYDDLTKHAAA
                     YRQMSLLLRRPPGREAYPGDVFYLHSRLLERAAKLSDNLGAGSLTALPIIETQAGDIS
                     AYIPTNVISITDGQIFLETNLFYQGIRPAISVGLSVSRVGGDAQTKAIKAVGGGLKLG
                     LAQFRELAAFSQFSTDLDPETRHTIERGQRLTELLKQPQFNPYAVWEMYASLYAATNG
                     AFDAVPVEKIKATQEALLRELKSKQAKLIEAVNTGDKPTDVQNDTILKVAKSVAETYK
                     STAKKAVKE"
     gene            4264..5130
                     /gene="atpG"
                     /locus_tag="VN778_02905"
     CDS             4264..5130
                     /gene="atpG"
                     /locus_tag="VN778_02905"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008578628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HXR49952.1"
                     /translation="MASTITLKRRITSIRNTRQITKAMELVSASKLRRAQEYAQRSRD
                     YRDLAYDLLRRLNAQAEVERMPLYAKRKVRNRLYIAITSNTGLAGAYNANILKLLTQG
                     LQKDQADGVKSHVITIGSKGAQLVRRLHDVDLLAVYPPFGDRPTANDVRPILNTIIQQ
                     YKGEAVDDVQILYTVFKSSIVQQAQSLQLLPAPVLEVDTGPQPISNFEPDVETVIEQV
                     TTRLVEAQVWQAILESLASEHAMRMIATKNATDNANELIDDYTLELNTARQAAITQEL
                     AEISGGAEALNG"
     gene            5123..5341
                     /locus_tag="VN778_02910"
     CDS             5123..5341
                     /locus_tag="VN778_02910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49953.1"
                     /translation="MANPELRGYYGEANFRLDAGAVPDLSVEKFNPYTDTEEKDFVRA
                     TINSVQGRKKIEQLKAHFNQELTTEGEA"
     gene            5341..>6008
                     /locus_tag="VN778_02915"
     CDS             5341..>6008
                     /locus_tag="VN778_02915"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008578629.1"
                     /note="Produces ATP from ADP in the presence of a proton
                     gradient across the membrane. The beta chain is a
                     regulatory subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HXR49954.1"
                     /translation="MATATKDKTRVGKITQIVGVVVDVEFSTDNLPAIYNALTVQLGD
                     LNLTMEVAQHLSESSVRAISLNSTDGLARGAEVVDTGAAIRVRVGEATLGHMFNVTGQ
                     PIDNKEDKFTERAPIHRTPPALIDQSGTVEILETGIKVIDLIAPIVKGGKVGLFGGAG
                     VGKTVLIQELINNIAKFHSGYSVFAGVGERTREGNDLYGEMEESGVLKNTSLVFGQMN
                     EPPGA"
BASE COUNT         1442 a         1512 c         1675 g         1379 t
ORIGIN      
        1 ccgtcaatgt cgatgaccgg aatgccccgc tgtccggatt tgtcgacggc ctctaaaccg
       61 gcggacggat cagtttcgac atctttatcg gtgtatttga cgcctttttg atcaaaatag
      121 tttttggcgg cgtggcaaaa cgcgcaccag gtagcgctgt aaacagtaat attcgtatta
      181 gacatagtat atctatgata gcactataca aaatatagtg caagcattgc gtgatctata
      241 tcacatctgt tataatgagt aagaaatgaa gcagacgacc gcgtcgccta ccccagaaga
      301 gaggaagtcg aatgcgccaa cggactctcg tagcccgggc gcagcttttg ttgtaatggc
      361 gctggatatg agctggcgac tggcgctgat cgtgcttatc ccaattctcg gcggatttca
      421 ggtggacaag aagctgaata cttctcctct ttttaccctc gtcggcttcg tactggccat
      481 ggctggaacg ggttatgtct tatggtgggc ctctaaaaaa gccgactccc tcgcaatgcc
      541 gaagggtgga gctaaatgat tgccggctta ctaacccgct tcgctatcga aggcagtccc
      601 accgtgcacg tggcgccggg caccgacttt acgatagccg gcatcccaat aactaacgcc
      661 atgttttacg gttggatttg caccatcagc atcattatct tcttgatctg ggcggcacgg
      721 aaggtgacgg tcaagccgca aggcggcgtg gtccagtacg tcgaagccgg cgtggagttc
      781 gtgactaact tcgttgaagg ttcgttcacg gatccggccg tcggtcgcaa atatgtgcca
      841 ttttttgtca ccctattctt ctttgtactt ttcaataact ggtcggagct gattccggtt
      901 gtgggcgacg gcattcagct gcacggtcat ccgcttttcc ggccagtcac cgccgatctc
      961 aatatgacgc tggccatcgg cctggtgacg atgctcgtag tgtatgccgc ttctatcaaa
     1021 gagactggcg gcccgattaa atatttgcgc cacttcttta ttggcaacct gctcaatccg
     1081 ctctacttag tgattggcat tattgagatt attacggatc tcacgcgtgt ctttagtctg
     1141 agtcttcggt tgttcctcaa tatcaccatt ggtgagattg tgattattgt cttcgccttc
     1201 ctcggtggcg ccttgttgcc gcacagtgta ttgtcgcctg tcacagcagt gccgttttgg
     1261 ctattggaac tgggcgtcgg tgtgctgcaa gcttatattt tcgctatttt aggtaccaat
     1321 tatttggcga ttgcagttaa cagtgcccag gatcataccc atgacgactt gaccgaaggt
     1381 ggtccacctg aaacaataga ggtagctgcc agggaggccg tcggtgggta agtttgatgt
     1441 tgcctctccg gctataggtg gactcggtgt cggtgggaca gtaataatag gtaataaata
     1501 aggagataca cgaatggtat ttgcagacgc agtcaatacg acaattatcg gtaaaggcct
     1561 gatgatcggc ggtggtttca tcggcccgac tattggtatc ggtctgatcg gcgccaatta
     1621 cttgcaagcc gtcggccgca atccggaagc ttcgcgcttc ttcggtcagg caatcatctt
     1681 tgttggtctg gttgaggtgt acggcttgtt ggccttcgct tcaacgttct ttgttaaata
     1741 gttttaggaa taattcacat taaatgattc aaacacttac attttttggt gacagcagtt
     1801 ccggcatcgg tgccctgggt gttgatggca aggccttcgt gattcagctc atcaccttct
     1861 tactggctta ttgggtgctc cagcggtatg ctttcggccc catcctaaag gttatgcgtg
     1921 agcgtcgtga gacgatcgag agcggcgtca agctgggtga gcagatgcaa aaggataaag
     1981 ccgctttgga agcgcaagtc gaagagacat tgcataaagc ccgtgagcaa gccgacggca
     2041 tcattacggg ggcgcaggat actgcccggc aaacggtgcg tgaagccgag gagaaagccc
     2101 gcgagaaagc ggccggtatc gtcgacgccg ccgaagctcg cattgagcaa gatactgcac
     2161 gcgctcgccg gcagctggaa aaggaaatcg ttggtctgat cactgatgcg acggaagcaa
     2221 ttatcgaaga aaaggttgat gctaagaaag atgcggagct cattgatcgg gcgcttaagg
     2281 agcggcaagc ggcatgaaac aatcgcggac taaaatttcc agggttattg ccgatcagac
     2341 tttggccggc ggtgatccta aacagctgag ccgggagctt gccgcgtact tgttaagcga
     2401 gcgtcgagtg agcgagttga gctctattct ccgagatgtg caggctgatt gggccgaagc
     2461 cggccatgtc gaagtgatcg cggcgagcgc ccatccgctg agcgcggaga ccacgtccga
     2521 aatcgagtgc caagtcaaac ggctgttgcc agatgctaag cagatcgttg tggttgaaac
     2581 acgcgatccg gcagttatcg gtggtgtgcg tttgaacctg gcaaatcagc agctcgacct
     2641 cagcatagaa gctaaattga ataagtttaa acagctaact acggcgggaa aggattaggc
     2701 gcgtggcaga actcacccta acagaattat cagaagatat ccgggcggca attgccgagc
     2761 tcaaagagcg cccggaaatc gaaaatgtcg gcgtcgtgac tcgcgtcggc gacggcatag
     2821 cctggattta tggcttaagc gctgccggct acagtgaaat gctggagatc gaaggtgttg
     2881 acagtcagca ggtcacagca tttgctttga acctggctga agacgagatt ggtgcggtta
     2941 tccttggtga tgacacccag gtaaaagccg gtgccaaagt gcgcctctct ggtaaggtgt
     3001 tggaagtacc agttggtcct gagcttgtgg gccgcatcgt tgaccccctc ggtaatccgc
     3061 tggatggcgg tgagccgatc aaagctaagc agaccagccg tgtggaacgt caggcgcccg
     3121 gtgtcctgga gcgtaagcat gttcacgaac ccatgatgac tggcctgatg gcaattgatt
     3181 ctatcgtccc gatcgggcgc ggccagcgtg agcttatcat tggtgaccgt cagaccggca
     3241 aaacggccat cgcggtcgac acgatggtta accaagcacg tcaaaagacc ggcgtgatta
     3301 atgtttacgt tgccgttggc caaaagctgt caaaggtcgc ggctttgcag gagcgcttga
     3361 agcgtgaggg cgttatggac cagactatca tcgtggccac tagtccggct gatccggctg
     3421 ccctgctcta cttggcgccc tacgccggtt gtgccatggg cgagtatttc cgtgataata
     3481 aacagcacgc cttgatcatt tacgatgatt tgaccaagca cgcggcggct taccgccaaa
     3541 tgtccttact gctgcgccgc ccaccaggcc gcgaagctta tcctggcgac gtgttctacc
     3601 tgcattcgcg attactggag cgcgcggcca aactgtcaga taacctgggt gctggttctc
     3661 taaccgcttt accgatcata gagacgcaag ctggtgacat cagcgcctac atcccaacga
     3721 atgtaatttc catcaccgac ggacagatct ttctagaaac gaacctgttc tatcagggta
     3781 tacgtccagc tatttcggtc ggcttgtcgg tttctcgtgt cggaggcgat gctcagacga
     3841 aggctattaa agccgtcggc ggcggcttga agcttggcct ggctcagttc cgcgaattag
     3901 cggccttcag ccagttctca accgacttgg accccgaaac ccgccatacc atcgagcgcg
     3961 gtcagcgcct cactgagcta ctcaagcaac cacagtttaa cccatatgct gtctgggaaa
     4021 tgtacgccag tctctatgcc gccactaatg gtgcttttga cgcagtgccg gtcgaaaaaa
     4081 ttaaagccac ccaggaagcc ctgctgcggg agttaaagtc gaagcaagct aagcttatcg
     4141 aagctgtcaa tactggcgac aagccgaccg atgtgcaaaa cgacaccatt ctcaaagtag
     4201 cgaagtcggt ggctgagact tacaaaagta ccgcaaagaa agcggtcaag gagtaattag
     4261 cgagtggcta gcacgataac ccttaagcgg cgcattacct ctatccgcaa cacgcggcag
     4321 attaccaagg ctatggagct ggtgtctgcg agcaaattgc ggcgagcgca ggagtatgcg
     4381 cagcgcagcc gtgattatcg tgatctagcc tacgacctgc tcagacggct caacgctcaa
     4441 gctgaggtag agcgcatgcc gctgtatgcg aagcgtaagg tgcggaatcg cctctacatc
     4501 gcgataacca gcaacaccgg cctggcggga gcgtacaatg cgaacatttt gaagctgctc
     4561 acgcagggcc tgcaaaagga ccaagctgat ggcgtcaaga gtcatgttat taccatcggc
     4621 agcaaaggcg cacaacttgt tcggcgcctc cacgacgtag acctgttagc cgtctatccg
     4681 ccattcggcg accgtccgac tgctaatgat gtgcggccga ttttaaatac gatcattcaa
     4741 cagtacaaag gtgaagccgt cgatgacgtg caaatcctct acaccgtgtt caaatccagc
     4801 attgtccagc aggcgcaaag tttgcagctc ttgccggctc ccgtgcttga ggttgatacc
     4861 ggcccgcagc cgatcagcaa cttcgagcca gatgtcgaaa ccgtcattga gcaagtcacc
     4921 actcgcctgg tcgaagccca agtctggcaa gcaatcctgg aaagcctggc cagtgagcac
     4981 gccatgcgca tgatagccac caaaaacgcg accgacaacg ccaacgagct gatcgacgat
     5041 tacactttgg aactaaacac cgcccggcaa gcggcaatca cccaagaatt agcagaaata
     5101 agcggtggag cggaggcatt aaatggctaa tccagagtta cgtggatatt acggtgaagc
     5161 taatttccgc ctagatgctg gtgcggtgcc tgatttatca gttgaaaagt ttaatccgta
     5221 tacagatacc gaagaaaagg attttgtgag agcgacaatc aattcagtac aaggacgcaa
     5281 aaagatcgag caactaaaag cacattttaa tcaagaactg accacggaag gagaagcata
     5341 atggcgacag caactaaaga taagactagg gttggtaaaa tcacgcagat cgtgggcgtg
     5401 gtggtggatg tggaattttc gacggacaat ctgccggcga tttataatgc gctcacggtg
     5461 caacttggtg atcttaatct gaccatggaa gtggcgcagc acttgagtga gtcgagtgtg
     5521 cgggcgattt cgctgaattc aaccgatggg ctggcacgcg gagcggaagt cgttgatacg
     5581 ggtgcagcca ttcgtgtgcg agtcggcgaa gctactttgg gtcacatgtt caacgtgacc
     5641 ggccagccga tcgataataa agaagacaag tttacggagc gggccccgat tcaccgcacg
     5701 ccgccggcgc tgattgacca atctggcacc gtggagattt tggaaaccgg tatcaaagtt
     5761 atcgacttga tcgccccgat cgtcaaaggc ggtaaggttg gcctcttcgg tggcgccggc
     5821 gtcggtaaaa ccgttttgat ccaggagctg attaacaaca tcgccaaatt ccactctggc
     5881 tactccgtgt tcgccggtgt cggtgagcgg actcgcgaag gcaatgacct ctacggcgaa
     5941 atggaagaat cgggcgtgct caaaaacacc tcgctggtct tcggccagat gaacgaaccg
     6001 ccgggcgc
//
LOCUS       DATNGP010000115         2887 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_1563122, whole genome shotgun sequence.
ACCESSION   DATNGP010000115 DATNGP010000000
VERSION     DATNGP010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2887
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1563122"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..780
                     /locus_tag="VN778_02920"
     CDS             <1..780
                     /locus_tag="VN778_02920"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXR49955.1"
                     /translation="IRRKALYATWHEVWGWRYWRQYAGRIIGTAAYLVERFSVRLPDH
                     IVAVSAQTASKLRSELQYQGPLSLVNNGIDRHHITTVKASNRQSDVIFAGRLLPHKHV
                     DILLAAIAEVRQRRPDIRCTIIGDGPDMVRLKRQARQLGIAARIRFEGFMEQPDDVLA
                     AMKASKVFVLPSSREGFGIALLEAYACGLRVVTVNAPDNAARHLVSSAAGAVCELNAR
                     ALAAAIEEQLNLPVPSEHAASAAADYDWQHSAEQLSEVYAI"
     gene            777..1910
                     /locus_tag="VN778_02925"
     CDS             777..1910
                     /locus_tag="VN778_02925"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HXR49956.1"
                     /translation="MKAMVITPYYYPIIGGLENYARQLNRALHKSEAWEIVVVTATKP
                     GQPSGTGRTDGQRVYRLKPWVVVSNTPLNPLWPFQIWRLKRREMPDVIIAHTPVPSMA
                     DAAALAAGKTPFFVVYHAASLLKPGSPIFNLLARLYRCVGWYTLHRAKHIFAVSDYVR
                     EQFSTRFQAKTTVVSNAVWPDEIQSRSQPAGGTHFVFIGSLDKSHSWKGLGHILQALA
                     IYQACYQPGARLTVIGDGNHRRSYENQVKVLGLADSVTFVGAKQGIAKARLLASATAL
                     VAYPTTSNDAFPTVILEAWAHHVPVIGAAIGPLPSLLKNQVNGYLAPAADPAALAEAM
                     RAVALAPRSQRANIARQAARRTRECYTWPQSAKQLGRSIGVVT"
     gene            1907..>2887
                     /locus_tag="VN778_02930"
     CDS             1907..>2887
                     /locus_tag="VN778_02930"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXR49957.1"
                     /translation="MKIVMLGSGSYKSSLTYFRLVALGQQLGRLGWGVSLIVPAADKY
                     NDFTPDWQATLEHVELVQPWQPATRRPLLNLLPYLISASLALLRRPAQLVYIYKPTPI
                     TIIGLLPRLLRRTPVILDLDDLGSMVMREEGRSGLSVRLVAACERLALRYATAVVVAS
                     RYLAQQVHDRYPDKPVLRLSNGVDPDEFVAPDRQSKPRPAIYYFGALNRLSLIKPLLV
                     ALPQTIQAVPETQVTIFGGGQALDEAKALVRKLGLEKSVTFTGRVKPEVVQQAVTFGD
                     IAVCIQPDIPTVQAASNVKVFQYMALASVPVVSEVGDLKQYIEAVDGPVGC"
BASE COUNT          616 a          823 c          792 g          656 t
ORIGIN      
        1 atccgccgca aggcactgta cgctacctgg cacgaagtgt ggggttggcg atactggcgg
       61 caatacgccg gccggatcat cggtaccgcg gcatacttag ttgagcgatt cagtgtccgt
      121 ttgccagacc acattgtggc cgtttctgcc caaaccgcca gtaaattacg ctcagaattg
      181 cagtaccagg gtccgctatc actcgtcaat aacggcattg accgccatca tattaccact
      241 gtcaaagcgt ccaatcgtca aagtgacgtg atttttgccg gccgcctgtt accccacaag
      301 catgtcgata tactgcttgc ggccatcgcc gaagtgcgtc agcgccggcc ggatatccgt
      361 tgcactatca ttggtgacgg accggatatg gtaagactaa agcgccaggc ccggcagcta
      421 ggcatcgccg cccggatccg ttttgaaggc tttatggagc aaccagatga cgttctggcc
      481 gccatgaagg cctcaaaagt ttttgttttg ccatcaagcc gcgagggttt cggtatcgct
      541 cttctcgagg cctacgcttg cggcctaaga gtggtcaccg ttaatgcccc ggacaatgcc
      601 gcccgccacc tggtctcgtc agcggccgga gcagtgtgcg aactgaatgc cagggccctg
      661 gctgcagcca tcgaagagca acttaacttg ccagtgccgt ccgaacacgc ggccagtgcc
      721 gcagcggatt atgattggca acacagcgcc gagcaactaa gcgaggtcta cgccatatga
      781 aagccatggt tatcaccccg tactattatc cgattatcgg cggcctggaa aattatgccc
      841 ggcaactaaa tcgcgcgctg cacaaaagtg aagcctggga gattgtggtc gtgactgcca
      901 cgaaaccagg acagccgagc ggcacggggc gaactgacgg ccaacgagtg tatcgcttaa
      961 aaccgtgggt cgtagtatcc aataccccac ttaatccttt atggccattt caaatctggc
     1021 gcctgaagcg ccgcgaaatg cctgatgtga tcatcgccca taccccggtg ccttcgatgg
     1081 cggacgcggc ggcattggca gccggcaaaa caccgttttt tgttgtttat cacgccgcca
     1141 gcctactaaa gcccggctcg ccgatattca atcttctggc acggctttat cgttgcgtcg
     1201 gttggtacac cttgcatcgc gccaagcaca tttttgctgt ttccgattat gtgcgcgaac
     1261 aatttagcac ccgcttccag gccaaaacaa cggtggtctc caatgctgtc tggcccgacg
     1321 aaatccagtc ccgaagccag ccggcaggtg gaacacactt cgtctttatt ggttccctcg
     1381 ataaatcgca cagctggaag ggtctcggcc acatcttgca agccttggct atatatcagg
     1441 cgtgttacca accgggggct cggctcactg tcatcggcga cggtaatcat cgaaggtcat
     1501 atgaaaacca ggttaaggtg cttggtttgg cggacagcgt cacattcgta ggtgccaagc
     1561 agggaatagc taaggcgaga ctgctggcaa gcgcgactgc tttggtagcc tatccgacaa
     1621 cttcgaacga tgctttcccg actgtcattt tggaagcttg ggcacatcat gtgccggtca
     1681 ttggtgcagc gataggaccc ttgccgagct tgttgaaaaa ccaggtcaat ggttacctag
     1741 ctccggcggc tgaccccgcc gcgctggcgg aagctatgcg ggccgtcgcc ctggcgccgc
     1801 gcagccagcg ggccaacatt gcccggcaag ccgctcgtag aacgcgcgag tgttatacct
     1861 ggccccagtc ggccaaacag cttggccgca gtatcggagt tgtgacatga aaatcgtcat
     1921 gctcggttcc ggttcctata agagcagctt gacgtatttc cgactggtag cactcggtca
     1981 acagcttggg cgactgggct ggggtgtttc cttgatcgta cctgctgctg acaaatacaa
     2041 tgactttact cccgactggc aggctacgct agaacatgtc gagttggtcc agccgtggca
     2101 accggcgaca cgtcgaccac ttctcaattt actcccgtac cttataagtg ctagcctagc
     2161 cttgctgcgc cgcccggcac agctggtcta tatttacaag ccgacgccca ttaccattat
     2221 tggcctcctg ccgcgtttac tgcgtcgcac gccggtcata ctcgatctgg atgatttggg
     2281 tagtatggta atgcgggaag agggacgatc aggcttgagc gtacgtttgg tagcagcctg
     2341 tgagcgccta gctttgcgct atgctaccgc cgtggttgtt gcttcgcggt acttggcgca
     2401 gcaagtacat gaccgttatc ctgacaagcc tgtgctacgg ctatcaaatg gggttgatcc
     2461 ggatgaattc gttgctcccg accgccagag caagccgcgg ccagccattt actactttgg
     2521 tgccctcaat cgcttatctt tgatcaagcc gctgctggtg gcactaccgc agaccataca
     2581 agccgtaccc gaaacgcaag tgacaatctt tggcggcggc caagcgcttg atgaggccaa
     2641 ggctctggtg cgcaagctag ggctcgaaaa gtcagtcact ttcacgggcc gggttaagcc
     2701 ggaagttgtc cagcaagccg taactttcgg cgatattgcc gtctgcatcc agcccgatat
     2761 cccaaccgta caagctgcca gtaatgtaaa ggtttttcag tatatggccc tcgccagcgt
     2821 accggtcgtc agcgaggttg gcgatctgaa gcaatatatt gaagctgtcg atggtcctgt
     2881 tgggtgt
//
LOCUS       DATNGP010000116         3205 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_3758319, whole genome shotgun sequence.
ACCESSION   DATNGP010000116 DATNGP010000000
VERSION     DATNGP010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3205
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3758319"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1411)
                     /locus_tag="VN778_02935"
     CDS             complement(<1..1411)
                     /locus_tag="VN778_02935"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HXR49958.1"
                     /translation="MGKPSKKNVGTIVKRSGVSFRVWAPFAKEVAVTGSFNNWGETPL
                     ESEGDGYWAVEVKGAEAGQEYKYVINTGYSKLVKNDPRALQLTTIAGNSVIVDTRFDW
                     EDDNFQPLPPERQVVYELHVGTFNRPDPATTGSFEHVIAKLDYLAELGITTIELMPIG
                     SMSMEREWWGYTPNYIYSVETLYGGRHQLLEFVKAAHKRGIAVVLDVVYNHFGPNNDL
                     DLWQFDGWSENGKGGIYFYNDWRSATPWGDTRPDYGRPEVRQFILDNIRLWLHDCHLD
                     GVRVDSTIFIRNAEGHNNDPATDISEAWSLLQRINTVAHKIKPGALTIAEDMGANNYL
                     TKPTSEGGAGFVSQWAVTFPYALRVALDAINDGDRNLNLIIEELGKVFNGNAFQRIIY
                     SDSHDSAANGSARLSEEISPGYPDSIYARERSLLAAAIVLTAPGIPMLLQGQEFMQGG
                     SFNDWQGLDWSKADRHAGII"
     gene            complement(1467..1832)
                     /locus_tag="VN778_02940"
     CDS             complement(1467..1832)
                     /locus_tag="VN778_02940"
                     /inference="COORDINATES: protein motif:HMM:NF021536.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2267 domain-containing protein"
                     /protein_id="HXR49959.1"
                     /translation="MGFRELIKKVQVYSGFSDAESKDALEMMVESLAVHLDDGERKDF
                     ASQLPQELQDIALAVYPTPENSRQDILDQFMEVERIDEGRAKKQIYAAWKALQEAVSE
                     GEMDHIRAQLPNSTVAFLH"
     gene            2114..2269
                     /locus_tag="VN778_02945"
     CDS             2114..2269
                     /locus_tag="VN778_02945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49960.1"
                     /translation="MADKNIDLFSSSHSSKRIKLAARKLGVTNLNEPAPPHRGLPSAD
                     QEQSDIM"
     gene            complement(2262..2750)
                     /locus_tag="VN778_02950"
     CDS             complement(2262..2750)
                     /locus_tag="VN778_02950"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF012413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HXR49961.1"
                     /translation="MIRLIAAIDRQRGMAKKGLQPWFIPADEAFFTEATKSEGGICLI
                     GSTTFKTFQHGPLPERQNYVLTHDETSIEGATLVHDLKQFMEDFRGKDVWIIGGANVF
                     AQVIDGDWADELYLTHIEADFGCDQFFPDYSGDFEPAEQSELQEQNGFIFSFASYAKK
                     VT"
     gene            2776..2852
                     /locus_tag="VN778_02955"
     tRNA            2776..2852
                     /locus_tag="VN778_02955"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2810..2812,aa:Arg,seq:tcg)
     gene            2860..2936
                     /locus_tag="VN778_02960"
     tRNA            2860..2936
                     /locus_tag="VN778_02960"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2894..2896,aa:Arg,seq:ccg)
     gene            2969..>3205
                     /locus_tag="VN778_02965"
     CDS             2969..>3205
                     /locus_tag="VN778_02965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49962.1"
                     /translation="MSKKKRISDGDVSFALFFLLSTIGFSWLFAGSINVRHNQSLLAI
                     LGFSILVYVVWEIIPQLYSPGQSIILFFLTFNLLP"
BASE COUNT          772 a          829 c          761 g          843 t
ORIGIN      
        1 tgatgatgcc ggcatgccgg tcggccttac tccaatccaa cccttgccag tcattaaagc
       61 tccccccttg cataaactct tggccttgca aaagcatagg gataccgggc gcggtgagca
      121 cgatggccgc cgccaaaagg gagcgttcac gagcgtaaat gctgtcggga taacccggag
      181 aaatctcttc gctcagccga gccgagccat tggcggcgct gtcatgcgaa tcgctataaa
      241 tgatgcgctg gaaggcgttg ccattaaata ctttgcccag ctcttcaata ataaggttca
      301 gattgcggtc gccatcgttg atggcatcta gggcaacccg gagagcatat ggaaaggtga
      361 cggcccactg gctaacgaaa ccagcaccgc cttcggaggt aggttttgtc agatagttgt
      421 tagctcccat gtcttccgcg atagtgagcg cgcctggttt gattttgtgc gctaccgtgt
      481 taatccgttg cagcaggctc caagcctcgg agatatcggt agccgggtca ttattatggc
      541 cttcagcatt acggataaaa atggtggagt caacccgcac gccatccaga tgacaatcgt
      601 gcagccacaa gcggatatta tcaaggatga attgacggac ttctggccgg ccataatccg
      661 gccgcgtgtc cccccagggc gtggccgagc gccagtcatt gtaaaagtaa ataccgccct
      721 taccattttc gctccaacca tcaaactgcc agaggtcgag atcgttattc ggaccgaaat
      781 ggttgtagac cacatcgagc accacagcta tgcctcgctt atgggcagct ttaacgaact
      841 ctagcaactg atgacgacct ccgtatagcg tttctactga atagatatag ttaggtgtgt
      901 agccccacca ttcccgttcc atcgacatgc tgccaatagg catgagttcg atcgtagtga
      961 tcccgagttc agccaagtaa tcaagtttgg caataacatg ctcaaagctg ccagtagtcg
     1021 cgggatcggg acggttaaaa gtaccgacat ggagctcata gaccacttgc cgttctggcg
     1081 gtaatggctg gaagttgtca tcttcccagt caaacctggt atcaacgatc actgaattac
     1141 cggcgatagt tgttaattgg agggcacgag gatcattttt gacgagcttg gagtaccccg
     1201 tgttaattac atatttatat tcttggccgg cctcagctcc ttttacttcg accgcccagt
     1261 aaccatcacc ttcactttcc agtggagttt caccccaatt attaaatgaa ccggtgacag
     1321 ccacttcttt ggcgaacggt gcccagactc gaaatgacac gcctgaccgc ttgacgatgg
     1381 ttcccacatt tttcttcgat ggcttaccca cacctactat tatgcttacg ctttagttca
     1441 agtgcaaggg gagaagggtg ccgttgttag tgcagaaagg ctacggtaga attcggtaac
     1501 tgagctctta tgtggtccat ctcgccttcg ctgacggctt cttggagcgc tttccaggcg
     1561 gcataaatct gcttcttggc acgcccttcg tcaatccgct ccacctccat aaactgatcg
     1621 aggatgtctt gtcgggagtt ttcgggagtt ggatagacgg cgagagctat gtcctgtaat
     1681 tcttgtggga gctggctggc gaaatcttta cgctcaccat cgtccagatg cacggcgagg
     1741 ctttctacca tcatttcgag ggcgtccttg gactcggcat ctgagaaacc agagtagacc
     1801 tgtacttttt taatcagttc tcgaaatccc atagctaccc tccttattct tggagcaggt
     1861 cagctgacag ccaatgtttg tccataacca cgtttaaagt caaaatatga ccgcgtgatt
     1921 ggccgacaat gcatgatgat ggcctcagac attttccctc ctggcgccct tttaggcatt
     1981 attatgaacg atccttcatt agcatatgga ctatgggata gacgttacta tgaagtttct
     2041 aacagttggg tattacactg acgaggtatc acgacccccg tatagtgaga tacgttcaac
     2101 aaggagtggc tctatggcag ataaaaatat cgatcttttc agctcaagtc acagctctaa
     2161 acgtataaaa ctggcggctc gcaagctcgg cgtcaccaac ttgaatgaac cggcccctcc
     2221 gcaccggggt cttccctcgg ccgatcaaga acagtcggat attatgtaac ttttttagca
     2281 tagctagcaa aagaaaaaat gaagccgttt tgctcctgta actcactctg ctcggccggc
     2341 tcaaagtccc ccgaataatc cggaaagaac tggtcacagc cgaaatcagc ttcgatatga
     2401 gtcaaataca gctcgtcggc ccagtcacca tctatcacct gagcaaaaac attcgctcca
     2461 ccaataatcc acacgtcttt cccacgaaag tcttccatga actgcttcag atcatgcacc
     2521 aacgtagcac cctcgattga tgtttcgtcg tgtgtcagta cgtagttttg ccgctcaggg
     2581 agaggcccat gctggaacgt cttgaaggtc gtgctgccta tcaagcaaat accgccttca
     2641 cttttggtag cttccgtaaa aaacgcctcg tcagccggaa taaaccacgg ctggagaccc
     2701 tttttggcca tgccccgttg gcgatcgata gcagcgatta atcgtatcat ctgttaagat
     2761 agtatacgac aacaagcgcc cgtagctcag cggattagag tgtctggctt cgaaccagaa
     2821 ggtcgcaggt tcgaatcctg ccgggcgtac caagcttttg cgcccgtagc tcagctggat
     2881 agagcgttgg tttccggtac caaaggtcgg gggttcgaat cccttcgggc gtaccaagca
     2941 taagcacttt ttatgtatac ttgttatcat gagcaagaag aagcgtatca gtgatggcga
     3001 cgttagtttc gcattgtttt tcctactttc aaccattgga ttcagctggc ttttcgcggg
     3061 ctcaattaat gtacgccaca atcaatcgct gttagcaata cttggctttt ctattttagt
     3121 ttatgtggtt tgggaaatta ttccccagct ttatagtccg ggacaatcta ttattttgtt
     3181 tttccttacg tttaaccttt tgccc
//
LOCUS       DATNGP010000117         5391 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_3371413, whole genome shotgun sequence.
ACCESSION   DATNGP010000117 DATNGP010000000
VERSION     DATNGP010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5391
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3371413"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..331)
                     /locus_tag="VN778_02970"
     CDS             complement(<1..331)
                     /locus_tag="VN778_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXR49963.1"
                     /translation="MILLDRVTKTYSRTAGPALDRVSLHVEPKEFVIVVGQSGAGKTT
                     LLRLLTREERPTSGKIIVGGIDYDKLKDKDIPLLRRKIGVVFQDFKLLPNKNVFENVA
                     FALEIVGI"
     gene            complement(362..832)
                     /locus_tag="VN778_02975"
     CDS             complement(362..832)
                     /locus_tag="VN778_02975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49964.1"
                     /translation="MPSNKPVNLLAIKEQRVDSYRKPYPSAGVGCIMLEGEMVEAIKR
                     QAEAEDFSPEKYIAHVFRTGLSIKSELDDDAGFSVRLSANEEPQTEEVFVSINDLTFA
                     GRECIMALDSAAKQKETSGPRVVYDLLAEAVGLQQPGEGVAEVVPFTSKRSTNI"
     gene            complement(851..1933)
                     /gene="prfB"
                     /locus_tag="VN778_02980"
     CDS             complement(851..1933)
                     /gene="prfB"
                     /locus_tag="VN778_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_138078638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HXR49965.1"
                     /translation="MNDERKRAEELKQEIAQAIERLNIAALQTQLQSLQVQSQQPGFW
                     DDSAQAQTVMKQIAKLETRVQPWLSLQKAITDANELLALDDDSLQAELGQQLGAIAAD
                     FAKLKEELKFNGPYDDHDAILSIYAGAGGTDAQDWTQMLFRMYSRYFENNRWRVAVVE
                     NSAGDEAGLKSITLEVEGPFAYGKLKGEHGVHRLVRLSPFNADNLRQTSFAKVEVMPK
                     IDRPDDLAIDDKDLKIDVYRSGGHGGQSVNTTDSAVRVTHVPTGITVAIQNERSQLQN
                     KETAMTILRGRLAQLQLEQHAEQLEQLKGPNQSAEWGNQIRSYVLHPYKQVKDLRTRY
                     ETSDPDAVLDGNLDDLINAYLENSFK"
     gene            complement(1950..3281)
                     /locus_tag="VN778_02985"
     CDS             complement(1950..3281)
                     /locus_tag="VN778_02985"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HXR49966.1"
                     /translation="MRHTVTEIKLKNGAKGLLVHIPDASVMTFDINFRAGEYLVDEQK
                     WEVPHLMEHVLLGANELLPKARDFQAELEKNGAYSNATTGVYDITYEAECADFEWDRV
                     LGLMLVAITKPLFLEEEFQAEFGNVQEEMAARSNNHFRRLSLEMRKALGLIAKTDAER
                     LEIMQNVTVSDVREHYLRTHHAPNMRFVIAGKLTPARKEAIEALLENIDLPSNSRRFA
                     LPHERPRHLKKPVYVPNDTVENLYFYIDMFARRRLSDPETDALNLVNTMLTETLYSKI
                     LGTARERGLVYGMNSGLSQTRDASNWWFGAQVSDKNAPQLMKIIVEELSKVQYGHIPE
                     EEIASTKQYALGRFQRSAQTVGGTAAGYAGRYFFDGVIEDYYRVPERIKAVTKESIIL
                     VANNMFTEKVWGLGVLGNCGQDFVGQLQDQITPLWQGPAAPLGSKVVAAWR"
     gene            complement(3287..>5391)
                     /locus_tag="VN778_02990"
     CDS             complement(3287..>5391)
                     /locus_tag="VN778_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HXR49967.1"
                     /translation="KEHDDERLRHLKPVSRQEAYGADITYGTNNEYGFDYLRDNMVRE
                     EDQLRQRELHYAIVDEVDSILIDEARTPLIISAPSVTSGTAYAQFAKVVRQLQKDKHF
                     ETDEKRKTVILTDAGVEKVEKILGIDNLYGSENIRTIYHLQQALRAHALFHRDKDYVV
                     TKDGEVVIVDEFTGRLLAGRRYNEGLHQAIEAKEGVEVQQESMTLATISFQNYFRLYE
                     KLAGMTGTALTEAEEFHQIYKLDVVEVPSNRQVARQDRPDRIYRTEKAKFKAIVREVK
                     ALHTKGQPVLIGTVSIEKNEALGQMLDKAGVPHQVLNAKNNEREAKIVAQAGEKGAVT
                     LATNIAGRGTDIVLGNGMKELGGLFVLGSERHESRRIDNQLRGRSGRQGDPGVTQFFV
                     STEDDLMRIFGGDRISNIMGRLNVDDDTPIENRIISRSLEGAQKKVEGYHFDQRKSVV
                     QYDDVMNRHRKAIYAMRREILRSPDISKRIKILVEEEAHGLAISPLVTTDQFEDLLRE
                     VFPFDEATLDRLFDTDATKFEKVLITEANELYSAREAAFTKDIMRKVERDIYLQVLDN
                     LWMQHLENMDHLREGIHWMSVGQQDPLVEYRRRGQLLFDEMQNTLRHDVVRTLFHAQP
                     IAQEDLDRAIDTELTRAAQGSVSNASQIITSGGEFDEGDFTDKKTAQATKKKQTSARK
                     KARKSERKRKTVAKRRRR"
BASE COUNT         1124 a         1401 c         1419 g         1447 t
ORIGIN      
        1 aaatgccgac gatctcgagt gcaaaggcca cattttcaaa gacgtttttg ttaggtaaaa
       61 gcttgaagtc ttggaaaacc acgccgatct tgcgccgcag cagcggaatg tctttgtctt
      121 tcaatttgtc ataatcgatg ccgccgacaa tgatcttgcc gctggtcggc cgctcttctc
      181 gggtcaacag ccgtaataat gtggttttgc cggctccgct ctgccccacc acgatcacaa
      241 actctttcgg ctcaacatgc aatgatacac gatcaagcgc cggccccgca gtacgactgt
      301 aggtcttcgt tactctgtcc aacagaatca ttagcgctat tatagcatca tttgtcgttg
      361 cttaaatatt agtcgaacgt tttgaggtaa acgggaccac ttcagccacg ccctcaccgg
      421 gctgctgtag tccaactgct tcagctaata gatcataaac gactcgcggt cctgaagttt
      481 ctttttgttt tgctgcacta tcgagcgcca taatacattc tctacctgca aaagttaaat
      541 cgttaatact aacaaaaacc tcctcggttt gaggttcttc attggctgag aggcgtacgc
      601 taaatccagc atcgtcatcc aattcactct tgatggataa gcctgtacga aaaacgtgag
      661 caatgtattt ttccggacta aagtcttcgg cttcagcttg gcgcttgatg gcttcgacca
      721 tttccccttc aagcataatg cagcctaccc ccgccgaagg gtatggcttt ctataagaat
      781 ctactctctg ttcttttata gcaagcagat ttaccggctt gttactaggc atgccttgat
      841 tataacacga ctacttaaac gaattctcga gataggcatt gataagatcg tcgaggttgc
      901 catcgagaac ggcgtccggg tcgctggttt cgtagcgggt gcgcaagtct ttgacttgtt
      961 tgtaaggatg cagcacgtag gagcggattt ggttgcccca ttcggctgac tggttggggc
     1021 ctttaagttg ctcgagttgt tcggcgtgct gttcgagctg gagctgcgcg aggcgaccgc
     1081 gcaagatcgt catggcggtc tctttgtttt gcagctggga gcgttcgttt tggatagcga
     1141 cggtaatacc ggtcggcaca tgcgtcacgc ggacggcgga atcggtggtg ttaactgatt
     1201 gcccaccgtg gccgccgctg cgataaacgt cgatcttgag atctttgtcg tcaatcgcga
     1261 ggtcatccgg gcggtcgatt ttaggcatca cttcaacttt ggcaaagctg gtctgacgaa
     1321 gattatcggc attaaaagga cttaagcgga ccaggcggtg cacgccatgt tctcctttca
     1381 atttgccgta cgcaaacgga ccttcgacct ccagtgtgat gcttttgagc ccggcttcat
     1441 caccggcaga attctctacc acagctactc tccatctgtt attttcaaaa taacggctgt
     1501 acattctgaa caacatttgt gtccaatctt gcgcgtcagt gccaccagcg cctgcgtaaa
     1561 tgctgagtat ggcatcgtga tcgtcataag gcccgttaaa tttcagctct tctttcagtt
     1621 ttgcgaagtc agccgctatg gcgcctagct gctggcccaa ttcagcctgc aggctatcgt
     1681 cgtcgagcgc taaaagctca ttagcatcag taatcgcttt ttgtaaactt agccagggct
     1741 gcacccgcgt ttctagcttg gcaatctgct tcataacggt ttgagcctgc gctgagtcat
     1801 cccaaaagcc cggctgctga ctctgaactt gtaagctttg cagttgcgtc tggagcgcag
     1861 caatattgag gcgctcgata gcttgagcaa tctcctgctt tagctcttcg gctcgttttc
     1921 gctcatcgtt catatctgaa gtataacaac taacgccagg ccgctacgac tttactgcca
     1981 agtggggcgg ctgggccttg ccagaggggg gtaatttggt cttggagttg accgacaaag
     2041 tcctggccgc aattgccgag aacgcctagt ccccagactt tttcggtaaa catgttatta
     2101 gctaccaaga tgattgattc tttggtgacg gctttgatgc gttccggtac ccggtaataa
     2161 tcctcaatca caccatcgaa aaagtagcgg ccggcatagc ctgcagccgt accgccaaca
     2221 gtttgggcac tgcgctgaaa gcgaccgagg gcgtattgtt tggtgctggc gatctcctcc
     2281 tcagggatat gaccgtactg cactttggag agctcctcga cgataatttt catgagctga
     2341 ggggcgtttt tgtcggagac ttgggcgcca aaccaccagt tactggcatc gcgcgtctgg
     2401 ctgaggcccg agttcatgcc gtagacaagc ccgcgctccc gggccgtgcc aaggattttt
     2461 gagtataaag tttctgtgag catggtattc accagattta gcgcgtccgt ctctggatcg
     2521 ctcaagcgcc gccgggcaaa catgtcaatg taaaaataga ggttttcgac tgtgtcgttc
     2581 ggcacgtaga ctggtttctt taaatgccgc ggacgctcat gcggcaaggc gaagcgccgg
     2641 ctgttagatg gcaaatcaat attctccagc agggcctcaa tggcttcttt gcgtgctggc
     2701 gttagcttac cggcaatgac aaaacgcatg ttgggagcgt ggtgcgtccg gagatagtgt
     2761 tcgcggacat cactaaccgt cacgttttgc atgatttcca gccgctcggc atcggttttg
     2821 gcgatgagac cgagcgcctt gcgcatttcc aggctaagcc ggcgaaaatg gttgttgctg
     2881 cgggcggcca tttcttcttg gacattaccg aattcggcct gaaattcttc ttctaaaaat
     2941 agcggtttgg tgatggcgac gagcatcaag ccgagcacac ggtcccactc aaaatcggca
     3001 cattcggctt cgtacgtgat gtcgtagaca ccggtggtgg cattgctgta ggcaccgttt
     3061 ttctcgagct cggcttggaa gtcacgtgcc ttcggtagca attcgttggc cccaagtagt
     3121 acatgctcca tgagatgcgg cacctcccac ttttgctcgt cgaccaaata ctcacccgcc
     3181 cggaagttga tatcaaacgt catgaccgag gcgtcgggaa tgtggacgag caagccttta
     3241 gcaccgtttt tgagcttaat ctcggttacg gtatgtcgca taagcgctag cgccgtctcc
     3301 ttttggcgac cgttttgcgt ttgcgctcgc ttttgcgagc ttttttgcga gccgaggttt
     3361 gcttcttctt ggtagcttgg gctgtttttt tatcggtgaa gtcgccctcg tcaaactcac
     3421 caccggaggt tataatctgg gatgcgttac tgactgagcc ttgagcggcc cgagtcagct
     3481 cggtgtcgat cgcgcggtca aggtcctctt gggcgattgg ctgagcatgg aaaagcgtgc
     3541 gcacgacatc gtggcgcaaa gtattttgca tctcatcaaa cagcagctgg ccacggcgcc
     3601 ggtattcgac tagcgggtct tgctggccga cgctcatcca gtggatgccc tcgcgcagat
     3661 ggtccatgtt ctcgagatgc tgcatccaga gattatcgag cacttggagg taaatatcac
     3721 gttccacttt gcgcataatg tccttcgtaa aagccgcttc gcgggcactg taaagctcat
     3781 tcgcctccgt tatcaagact ttttcaaact ttgtcgcgtc ggtgtcgaat aagcggtcca
     3841 aagttgcttc atcaaacgga aagacttcgc gcagtaaatc ttcgaactgg tcagtagtaa
     3901 ccagcggcga aatggccagc ccgtgcgctt cttcctcgac taagatcttg atgcgtttac
     3961 tgatatccgg cgagcgcaag atctcgcggc gcatggcata aatagctttg cggtggcggt
     4021 tcatgacatc gtcgtactgc accacacttt tacgctgatc aaagtgatag ccttcgactt
     4081 tcttctgggc accttccaac gaacggctta tgatgcggtt ttcgattggc gtgtcgtcat
     4141 cgacattgag gcggcccata atattcgaaa tacggtcgcc gccaaaaata cgcattagat
     4201 catcttcggt actgacaaag aattgtgtca cgccgggatc accctgacga ccggaacggc
     4261 cgcgcagctg gttgtcgatg cggcgcgatt cgtgacgctc gctgcccaag acaaaaaggc
     4321 cgccgagctc tttcatgccg ttgccaagca cgatgtcggt accgcgaccg gcaatgttgg
     4381 tggctagcgt cacggcgccc ttctcgccgg cttgggcgac aattttagct tcacgctcgt
     4441 tatttttggc gttcaaaact tggtgtggta cgccggcttt atcgagcatt tggccgagcg
     4501 cttcgttttt ctcgatcgaa acggtaccaa tcaggacagg ctggcctttg gtatgcaatg
     4561 ctttgacttc acggacgatg gctttaaatt tggctttttc ggtacggtaa atgcggtctg
     4621 ggcgatcctg gcgtgctacc tgacggttcg atggcacttc gacgacatcc agcttataaa
     4681 tctggtgaaa ctcctcggct tcggtcaggg ctgtaccggt catgcccgcc agtttttcat
     4741 ataagcggaa gtaattctgg aaggaaatgg tcgccagtgt catgctttct tgctggacct
     4801 cgacaccctc tttggcttca atggcttggt gcaagccctc gttgtagcgg cggccagcca
     4861 agaggcgccc ggtgaattcg tcaacaatca ctacttcccc gtctttggtc accacgtaat
     4921 ctttgtcgcg atggaagagc gcgtgggctc ggagcgcttg ttgcaggtga taaatggtgc
     4981 gaatattctc tgagccgtac aagttgtcga tgccgaggat tttctcgact ttttcgacac
     5041 cggcatcggt caagatgacc gttttgcgct tttcgtcggt ttcgaaatgc ttgtctttct
     5101 gcagctgacg cacaaccttg gcaaactgag cgtaggctgt gccgctagtc acgctgggtg
     5161 cactaataat cagcggggtg cgagcttcgt cgatcaagat ggagtcaact tcgtcgacaa
     5221 tggcgtagtg cagctcgcgc tgccgcaatt gatcttcctc gcgcaccatg ttgtcgcgca
     5281 agtagtcaaa gccgtattcg ttgttggtgc cataagtgat gtcggccccg taggcttcct
     5341 gacgcgaaac cggcttcagg tgcctgagac gttcgtcgtc gtgctcttta t
//
LOCUS       DATNGP010000118         2696 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_393641, whole genome shotgun sequence.
ACCESSION   DATNGP010000118 DATNGP010000000
VERSION     DATNGP010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2696
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_393641"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..942)
                     /locus_tag="VN778_02995"
     CDS             complement(<1..942)
                     /locus_tag="VN778_02995"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HXR49968.1"
                     /translation="MQDTIVELITQAQKILVVQADNPDADSLGSALALEHILGDMGKD
                     VYLYCGVDVPTYLHYIAGWDRVRNELPSNFDLSIIVDANTYTLFGQVEKHGKWGVLKS
                     KPCIVLDHHGSVDKPLDFAAAAIIDDSAASTGEIIFHFAAKSDWPISVAAAEHIMSTI
                     LGDTQGLTNDKTTADTFRIMAELTDKGVNRAMLEELRREYSRMPESIFRYKASLIQRM
                     EFAGGGRIAHVVIPQDEINEYSPLYNPAVLVQFDSLQVTDVQLSVVFKTYDDGKITGK
                     LRCNYSAPIADKLAEHFGGGGHPHSAGFKVTDGRPFNE"
     gene            997..1287
                     /locus_tag="VN778_03000"
     CDS             997..1287
                     /locus_tag="VN778_03000"
                     /inference="COORDINATES: protein motif:HMM:NF019481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1653 domain-containing protein"
                     /protein_id="HXR49969.1"
                     /translation="MEKIPYDQLIDTLAAARIKVTPGTHWRHYKGNEYLVKDIVLIEA
                     TNEVAVLYASLEQPAVAFVRPFTSWHEQVDIGGRLISRFSKATETTAGVPIA"
     gene            complement(1291..1896)
                     /gene="recR"
                     /locus_tag="VN778_03005"
     CDS             complement(1291..1896)
                     /gene="recR"
                     /locus_tag="VN778_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010165493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HXR49970.1"
                     /translation="MQILPPALTNLIEALGNLPGVGPRTAERYAYYLVRREPAKAEQL
                     SQALTTLHAGIKYCPQTFALISSDDAVSPLYSDPRRDKKLIAVVAEPLDIVALEKTNG
                     FHGTYHVLGGLVSPIDGIGPEQLHIRELVKRIDDDAVEEIILATNASVEGESTALYIQ
                     QQIGDRTVKITRLARGLPIGVDLEYADQITLSRALEGRQTL"
     gene            complement(2088..2381)
                     /locus_tag="VN778_03010"
     CDS             complement(2088..2381)
                     /locus_tag="VN778_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015642082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HXR49971.1"
                     /translation="MSRFDQAKMLMRVKKIQKELQKQIISVEQGDGAVRVEITGEQKL
                     KKVHIDPKAIDLDDIGQLERWIEDAVKDAIQQSQKIAAEKMQPMMGALGDLGL"
     gene            complement(2463..>2696)
                     /locus_tag="VN778_03015"
     CDS             complement(2463..>2696)
                     /locus_tag="VN778_03015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49972.1"
                     /translation="GLASLIPLLYLFIIHGLRQLLGQWLTVFPRNPIARFTGIGIICL
                     MLCFSILYQVRAYFVAWPHTSATLQTYTQLPPQ"
BASE COUNT          642 a          655 c          712 g          687 t
ORIGIN      
        1 ctcgttgaac ggacggccgt cggttacctt aaaaccggca ctgtggggat gcccaccacc
       61 cccgaaatgc tcggctagct tatcggcgat cggggcactg taattgcagc gcagtttgcc
      121 ggtaattttg ccgtcatcgt aggttttaaa taccaccgaa agctgtacat cggtaacttg
      181 cagggaatca aactgcacta gcactgccgg attgtacaag ggactatact cgttaatttc
      241 gtcctgaggg atcactacat gtgcgatacg gccacccccg gcaaactcca ttcgttgaat
      301 caaggacgcc ttgtaacgga aaatactttc tggcatacgg ctgtattcgc gtcgaagctc
      361 ttcgagcata gcgcggttga cgcccttatc agtcagctcg gccatgatac ggaaggtatc
      421 ggcggtggtt ttgtcgttgg taaggccctg agtatcgcct agaatggtgc tcatgatatg
      481 ctctgcggct gctacactaa taggccagtc gctctttgct gcgaagtgaa aaataatttc
      541 gccggtcgaa gcggcgctat catcaatgat agccgcggcc gcaaaatcta acggcttgtc
      601 gacggagccg tggtgatcca gcacgatgca tggcttgctt tttagaacgc cccacttgcc
      661 gtgcttttca acctgcccga aaagcgtgta agtgttggca tctacgataa tggagaggtc
      721 aaagttgcta ggcagctcat tacgtacccg gtcccaaccg gcaatataat gaaggtacgt
      781 aggtacatca acaccgcagt acagataaac atcttttccc atatcaccca aaatatgctc
      841 gagcgctagg gcgctgccta ggctatcggc gtcaggattg tcggcctgta cgaccaggat
      901 tttttgagct tgggtgatca gttcgacgat agtatcttgc atctttgcta ttttaccagt
      961 tttagccggc aatagcttct gttaagctta cagtatatgg aaaaaatacc gtacgaccaa
     1021 ctcattgaca cattagctgc agctcgaatc aaagttacgc caggtacgca ttggcgccat
     1081 tacaaaggta atgaatacct ggtgaaagac atagtactga ttgaagcaac gaacgaagta
     1141 gcggtattgt atgcatcgct tgagcaacct gccgtggcat ttgtccgtcc gtttaccagt
     1201 tggcatgaac aagttgacat cggcggtcgg ctgatcagtc ggtttagtaa ggctactgaa
     1261 accactgcgg gcgtgccaat tgcctagagc ctagagtgtt tgacgacctt cgagggcgcg
     1321 actaagggtg atctggtcgg catattctaa gtcaacgccg ataggtagac cgcgggccaa
     1381 gcgggtaatc tttaccgttc ggtcaccgat ttgttgctga atatagagcg cggtggattc
     1441 accttccacg ctagcgttag tagctagaat gatttcctcg accgcgtcgt cgtcgatacg
     1501 cttaactagc tcgcggatgt ggagttgttc gggcccgata ccatcgatag ggctcaccaa
     1561 accgcccagt acgtggtaag tgccatgaaa gccgttggtt ttttcgagag ccacaatatc
     1621 aagcggctcg gccacgacgg caatcagttt tttgtcacga cgagggtcgc tatacagcgg
     1681 cgagacagca tcatcactgc taataagtgc gaaggtttga gggcagtact tgatgccggc
     1741 atgcagcgtg gtgagcgctt gggacagctg ctcggcttta gccggctcgc ggcgcaccag
     1801 atagtacgcg tatcgctcgg cggtacgggg accaaccccc ggcagattgc caagagcttc
     1861 gatgaggtta gttagggctg ggggaagtat ttgcatagta attagcgctg atgatcgcct
     1921 gtggttaatc ctttactggc tgaaaactct gttttcggct gcgtctccta tagtctaaag
     1981 acaaaccctg aagggttttt ctacgcttcg tcaggctacg cctgacttgc tacttttccg
     2041 gtcttcagac cgaacgtcgg tgaatcagga tttatcgtct gagtagtcta cagtcccaaa
     2101 tcgcccaacg cgcccatcat aggctgcatc ttttcggcgg cgatcttttg gctctgctga
     2161 atagcgtctt tgacagcatc ttcgatccag cgctctaatt ggccaatatc atcaagatcg
     2221 atggccttgg gatcgatgtg gaccttttta agcttttgtt cgccggtgat ttcaacacgg
     2281 acggcgccat caccctgctc tacagaaatg atctgttttt gtagctcttt ttgaattttt
     2341 ttaacacgca tcagcatttt ggcctggtcg aatcgagaca tataaaactc ccctttagat
     2401 ggattatggt gaacggtact gcatatccct gccgttcaac cgtctatact attgtatcag
     2461 atttactgag gtggcaactg ggtatatgtt tgcagagtgg ccgaggtgtg tggccaggct
     2521 acgaagtagg cgcgcacctg gtaaaggatc gagaagcaga gcattagaca gatgatgcca
     2581 atgccagtga accgagcaat agggttgcgc ggaaaaacag tcaaccattg gccaagcaat
     2641 tgccgaagtc cgtggatgat aaacaggtat aggagtggaa taagactggc aagccc
//
LOCUS       DATNGP010000119         2717 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_374717, whole genome shotgun sequence.
ACCESSION   DATNGP010000119 DATNGP010000000
VERSION     DATNGP010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2717
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_374717"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..757
                     /locus_tag="VN778_03020"
     CDS             <1..757
                     /locus_tag="VN778_03020"
                     /inference="COORDINATES: protein motif:HMM:NF025433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LssY C-terminal domain-containing protein"
                     /protein_id="HXR49973.1"
                     /translation="FVDRRLRLPDIISWLITYVVAAYVLVPALIRFIRIVRPPKHVPL
                     YCVTPDGFASDPINIGIIATRRELLMAMERAGWYIADPHTPRYLGRQILSTIFGWSYS
                     NAPVSSLYLFGRKQDIAFEIPLGAGSAGRHHVRFWATTYEGGKKSMSFRSIHWHHRRA
                     HVFGDNLLWVGASSLDVGVSVIRHNFQFTHMIDPDTNLERDLIVEQLRSQKLVKKVET
                     IKLGDPYRLINRVWRGSLHTDGKMAVVTLRKKV"
     gene            777..1085
                     /locus_tag="VN778_03025"
     CDS             777..1085
                     /locus_tag="VN778_03025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49974.1"
                     /translation="MYKFSNIKNMTAPEQPNPEIPLNIRFLECANWLVNRIEGKTTQE
                     LDNKDFRLLSASTLAETETSTILACQVGGCSVQWAMNKNSDGTFSREMQVGLSVCPSQ
                     "
     gene            1151..1636
                     /locus_tag="VN778_03030"
     CDS             1151..1636
                     /locus_tag="VN778_03030"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HXR49975.1"
                     /translation="MTFKKLSSEELRKHKGVSFTGITTVFFCHDGQGKLLLGKRSKNA
                     RDEHGRWDPGGGGLKHGQSVEESMKREILEEYGTVSKRVDFIGYFDAFRTSTDGLPTH
                     WLAMCFAVLVDPQQVKLNEPDILDEIGWFTLDDLPSPLHSQSHIFLEKHGDKLKECMG
                     L"
     gene            complement(1631..>2717)
                     /locus_tag="VN778_03035"
     CDS             complement(1631..>2717)
                     /locus_tag="VN778_03035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49976.1"
                     /translation="IQVTSNQVAANMIQLTPNGHLYFLSNLSGTIDVVKTNLDGSGRK
                     TIFAGTGKEDPNTTNLLASRDWHYLVLKSQHDSSRAALYLIDTSDDSVTSFDTSNADF
                     TLIGWINHTFVYDIASNTIPASQAGHEIIKGYDADHQQLNQYDQDQAEGTADSYDYQN
                     FSNFYILNNELAYTTQWYSAGGYDVSSKSDTIRVVQPGIQNKKDYQSVSAANIVYIQA
                     ALYEPQAVYYGFYNGSTNQTTFYAFENQSVNTASNLTGSDLNKAYPTYLLSPSSNRTF
                     WTELRDGKNTLFLGDNDAKNAKQIASLSDYSPYGWFTDSYVLVSKNASELYIMPAGST
                     NAQPLKITDYYKPAQTFNGYGYGYGGL"
BASE COUNT          609 a          646 c          742 g          720 t
ORIGIN      
        1 gtttgttgat cgccgcctgc gcttacctga tatcatatct tggctcatta cgtatgtcgt
       61 cgcagcttat gtactcgtcc ccgctttgat tcgcttcatc cgtattgtga ggccgccgaa
      121 gcacgtgccg ctatactgtg tgacgcctga cggcttcgct tcggatccga taaatattgg
      181 catcatcgcg acccgccgcg aactcctcat ggctatggag cgtgccggct ggtatattgc
      241 cgatccccac accccgcgct atcttggtcg acaaatactc agcactatat ttggctggtc
      301 gtattcgaac gcaccagtca gcagcctcta cctgttcggc cgcaagcaag atattgcctt
      361 tgaaatcccc ctgggtgccg gctcggctgg acggcatcac gtgcgctttt gggccacaac
      421 gtacgagggc ggcaagaagt cgatgagctt tcgcagcatc cattggcacc atcgccgcgc
      481 gcacgttttc ggtgacaatt tgctctgggt tggcgcgtct tccctcgatg tcggcgtcag
      541 cgtcatccgc cacaacttcc aattcactca catgattgac ccggatacta atctagagcg
      601 cgacctaatc gtcgagcaat tgcgttcgca aaagctagtc aaaaaagtcg agaccatcaa
      661 gctcggtgac ccctaccgcc taatcaaccg cgtctggcgc ggctccctcc acactgacgg
      721 caaaatggct gtcgtgaccc tgcgcaaaaa agtttaattc cttatgcttg caatagttgt
      781 ataaatttag caatattaag aacatgactg ctccagagca acctaatcca gaaatccctc
      841 tgaacattag atttttagag tgcgctaatt ggttggttaa tagaattgaa ggtaaaacta
      901 cacaagagtt ggataataaa gactttcggt tactttcagc ctctactctg gccgagacag
      961 aaacttcaac gattttagct tgccaggttg gtggctgctc tgttcaatgg gcgatgaata
     1021 agaattcaga cgggacgttt agcagggaaa tgcaggtcgg cctttcggtt tgtccatccc
     1081 aatagctcag cagcctaact ccgcatcctg cctgaacttt tccgtagttg acttgttatg
     1141 attatagata atgacgttta agaaactctc gagcgaggag ctcaggaaac acaaaggggt
     1201 aagcttcacc gggattacga cggtgttttt ctgtcatgac ggccagggta agttattgct
     1261 tggtaaacgt agcaaaaacg cgcgggatga gcatggacgg tgggatccgg gcggcggggg
     1321 gctgaagcat gggcagtcgg tcgaagagag tatgaagcga gaaatcctgg aagagtatgg
     1381 caccgtgtca aaacgcgtag atttcattgg ctacttcgac gcttttcgga cttcgacaga
     1441 tggcttgccg acacattggt tagccatgtg ctttgcagtg ctcgttgatc ctcaacaagt
     1501 taagctcaat gagccggata tacttgatga aatcggctgg ttcacactcg atgatctgcc
     1561 aagccctctg cattcacagt cccatatttt ccttgagaag catggcgaca agctaaaaga
     1621 atgtatgggg ttataggccg ccgtagccat agccgtagcc gttgaaggtt tgagcgggct
     1681 tgtagtagtc agttattttg agcggttggg cattagtgct gccggcgggc atgatataga
     1741 gttcgctggc gtttttggaa actaagacgt agctgtcggt gaaccagcca tagggggagt
     1801 agtcgctgag gctggcgatt tgcttggcgt tcttagcatc gttgtcgcca aggaagagcg
     1861 tgttcttgcc gtcgcgtagt tcggtccaga aagttcggtt gctgcttggc gagagaaggt
     1921 aggttggata ggctttgttg aggtcgctcc ccgtaaggtt gctggccgta ttaaccgact
     1981 gattctcaaa agcgtaaaac gtcgtttggt tggtgctgcc attgtaaaaa ccatagtaaa
     2041 cggcttgtgg ctcatagagc gccgcttgaa tgtatactat gttggccgct gagaccgatt
     2101 gatagtcttt cttgttttgt atgcccggct gcaccacgcg gatggtgtca gacttgctac
     2161 taacgtcgta accgccagcg ctgtaccact gggttgtgta agctagttcg ttattcagaa
     2221 tatagaaatt ggagaagttc tggtagtcgt aactgtccgc cgtgccctcg gcttggtctt
     2281 gatcgtactg gttcagctgc tgatggtcag cgtcgtagcc tttgatgatt tcgtgcccgg
     2341 cctgcgatgc cggaatggtg ttacttgcga tgtcgtagac gaaagtatga ttgatccaac
     2401 cgatgagcgt gaagtcggca ttgctggtat caaaggaagt gacgctgtcg tcactggtgt
     2461 cgattaggta gagtgcggcg cggctgctgt cgtgttgaga tttcaggacc aggtagtgcc
     2521 aatcgcgtga agccagcagg ttcgtcgtat taggatcttc tttacccgta ccagcaaaga
     2581 tcgttttccg cccgctgcca tcaaggttgg tcttaacgac atcaatcgtg ccgctcaggt
     2641 tactgaggaa gtaaagatgg ccgttgggcg tcagctggat catgttggca gcaacttgat
     2701 tgctcgtcac ctgaatg
//
LOCUS       DATNGP010000120         2557 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_1589476, whole genome shotgun sequence.
ACCESSION   DATNGP010000120 DATNGP010000000
VERSION     DATNGP010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2557
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1589476"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..481
                     /locus_tag="VN778_03040"
     CDS             <1..481
                     /locus_tag="VN778_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008578480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXR49977.1"
                     /translation="AKAQLHGILLPTDVSEYLATNVPTNVRELEGALNQLLAVCEMRG
                     VEPSLALITGLISTTKARPKHLSAKQVIERTAKHFQIPLEDMLGPKRDKDIVVPRQVA
                     MYMLRSELHLSFPKIAHELGRKDHTTAIHSVEKIEKEVNFNNDLRTAIADIKERLYA"
     gene            745..1854
                     /gene="dnaN"
                     /locus_tag="VN778_03045"
     CDS             745..1854
                     /gene="dnaN"
                     /locus_tag="VN778_03045"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008578475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HXR49978.1"
                     /translation="MAGENNMKLQVTQENLNHALGTVARVANARNPLPVLANVLIRAD
                     HNRLSIAATNLDIAITQFVGAKISDEGSITVPARLMQDFISSLPSGVIDLTLEETQLH
                     IATNQYKSVINGIVADDFPVMPTITGGTSWTIPAPLLKKGLQQVVFAASSDESRPVLT
                     GVLLHTVEGVLYMAATDSYRLAEKSLGANKTAINLLIPASAMQDLLRIIGDVEDEVKV
                     THDDQQVRFQVGDVELVARLLEGKYPDYRKLIPQKFAVSATIKRADFINVTKVSALFA
                     RESAGSVTIDVDEKTNSLSIRSIASQLGENTATANGVVTGNGSITLNSRYLLDALQAL
                     NADEVMFGFNGKLEPTLLHDAGQTDYGHIIMPLKS"
     gene            1874..2179
                     /locus_tag="VN778_03050"
     CDS             1874..2179
                     /locus_tag="VN778_03050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49979.1"
                     /translation="MATKVGAKRVIANRTLSRVIFDDGMYVVALVTPVLTLPQVFLIW
                     SGRQTAGVSLPTWAAYTTMSGVWLIYGILQHQKPLVISQVCLFVVDFAIVLGIALFR"
     gene            2197..>2557
                     /locus_tag="VN778_03055"
     CDS             2197..>2557
                     /locus_tag="VN778_03055"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXR49980.1"
                     /translation="MQQFRSYADASFEFDGSVNIIVGPNASGKTNLLEALLVLAEGRS
                     YRVEDAELVLHGALWARIDGLVDDAERTLKLTTDAVVKKTYEIDGRVFQCLSLERTLP
                     VVLFEPNHLDLLQGSPER"
BASE COUNT          626 a          672 c          666 g          593 t
ORIGIN      
        1 ggccaaagcc caattacacg gcatactgtt gccgaccgac gtcagtgaat atctcgctac
       61 caatgtgccg acgaacgtcc gcgaactcga gggcgcactc aaccagctgc tggcagtctg
      121 cgaaatgcgc ggcgtcgaac catcgctcgc ccttattacc ggcctcatca gcacaacaaa
      181 ggcccggccc aaacacctca gcgccaagca ggtgatcgag cgcacggcca agcactttca
      241 gattccgcta gaggacatgc tcgggccaaa gcgcgacaaa gacatcgtcg taccccggca
      301 ggtagccatg tacatgctac gaagcgagtt acaccttagc tttcctaaaa tcgctcatga
      361 actgggccgc aaagaccaca cgaccgccat ccactcggtc gaaaaaatcg agaaagaagt
      421 caacttcaat aacgacctcc gtacggctat cgccgacatc aaggagcggc tctatgcgta
      481 agtacctggg catagcctgt ggacagacgg tgagttgtgc ttgtataagt gcgcatgaac
      541 tagctcgagc ttccacacat gggtatgcga aactctctga ctggtggtta aaccgtgcac
      601 aaagtaggtt agttatgagc cgtctgttcg ccgcttttgc acaccaaaag tacccagatt
      661 ttcagataga atatgcgact tatactcatt ctccacagga cctatgataa gcaacgataa
      721 gctggattat attaaagaaa ggaaatggct ggggaaaaca atatgaagct acaggtaact
      781 caagaaaatc tcaatcacgc actcggcacg gtcgctcggg ttgctaatgc tcgtaatcct
      841 ctgccggtgt tggctaatgt ccttattcgc gcggaccata accggctcag tatcgcagcg
      901 accaacctgg atattgcgat tacgcaattt gtcggtgcca agatcagtga cgaaggttcc
      961 attaccgttc cagcccggct gatgcaggac ttcattagta gtctgccgag tggcgtgatc
     1021 gacctgacgc tcgaagagac tcagcttcat attgcgacta accagtataa gtcagttatc
     1081 aacggcatcg tagcagatga cttcccggtc atgccgacga ttacgggtgg cacctcgtgg
     1141 acaattccgg cgccgcttct caagaaagga ttgcagcaag tagtgtttgc cgcctccagt
     1201 gacgagtcgc ggccggtttt gacgggcgtt ctactccata cggtcgaagg tgtgctctat
     1261 atggcagcga ccgacagcta ccgcttggcc gaaaaaagcc tcggtgccaa caagacagcg
     1321 atcaacctgc ttattcccgc ctcagcaatg caggatttgc tgcgaatcat cggcgacgtt
     1381 gaagacgagg tcaaagtgac ccacgacgat cagcaggtac ggttccaggt aggtgatgtc
     1441 gagctggtgg ctcgcttgct cgaaggtaaa taccctgact accgtaagct gattccccag
     1501 aaatttgccg tcagcgctac tatcaaacgg gctgatttca ttaacgttac gaaggtctcg
     1561 gcgctgtttg cacgggagag tgccggcagc gtgacgattg acgttgacga gaaaacgaat
     1621 tccttgagca tccgctctat cgcctcgcaa ctcggcgaaa atacggcgac ggccaatggc
     1681 gttgtgactg gtaacggcag tatcacgctg aactcgcgct acttgcttga tgcactccaa
     1741 gccctgaatg ccgatgaagt aatgtttggt ttcaacggca aactcgagcc aacgctgctg
     1801 catgatgccg ggcagacaga ctacgggcat atcatcatgc cccttaaaag ctagctgtta
     1861 gaatagaatg tatatggcaa cgaaggtagg cgccaagcga gtaattgcta atcgaaccct
     1921 ttctcgcgtt atatttgatg acggaatgta tgtcgtagcg ctggttacgc cagtgttaac
     1981 gttgccgcag gtgtttctta tctggtctgg ccggcaaact gctggcgtat cactaccgac
     2041 ctgggcggcc tacacgacga tgtccggagt ctggcttatc tatggaatcc tgcagcacca
     2101 aaagccactg gtaatatccc aggtgtgctt atttgttgtg gactttgcga ttgttttggg
     2161 cattgccttg tttcgttgat gatccacagt cttcgtttgc aacagttccg ttcgtacgct
     2221 gacgcctcat ttgaatttga tggcagcgtt aacattatcg tcggaccgaa cgccagcggc
     2281 aaaaccaatt tgctcgaagc cctgctggta cttgccgaag gtagatcgta ccgcgtggaa
     2341 gatgctgagc tggttctgca cggcgcgctg tgggcgcgta tcgacggctt ggttgatgac
     2401 gctgagcgta cgctaaaact cactactgat gctgttgtca aaaagactta tgaaattgac
     2461 ggccgggtgt ttcagtgtct gagcttagaa cgcacgctac cggtagtgct ttttgaaccc
     2521 aatcatttag atttgctcca aggctcgccc gagcgcc
//
LOCUS       DATNGP010000121         2104 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_275981, whole genome shotgun sequence.
ACCESSION   DATNGP010000121 DATNGP010000000
VERSION     DATNGP010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275981"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1422
                     /gene="dnaX"
                     /locus_tag="VN778_03060"
     CDS             <1..1422
                     /gene="dnaX"
                     /locus_tag="VN778_03060"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR02397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HXR49981.1"
                     /translation="LDEVIGQEHITETLKRAIKSGRVSHAYLFTGPRGVGKTSIARIL
                     AHELNEVAYEGEDAHLDIIEIDAASNGGVDEIRDLRDKIYIAPANGKYKVYIIDEVHM
                     MTTSAFNALLKTLEEPPAHAIFILATTEAHKLPATIISRTQRFTFKPIAHDKMVKHLR
                     TIADTEKIAIDDEALDLIAELSDGGFRDAIGMLDQASGSAKKIDRAAIEALLGIPTEA
                     STTQILDLIQAHDASSLAAQLAELRANGFQASAVAKQLAQHLRSGLLAGKPALEPINL
                     LTLLDRLVDVPASHNADQLLEIVLLEAALRGAAAPMPEAAPSVSIAPPAPIPAPMPPS
                     EHKPAQKPSEPAAGAEADTPSSNKLIDLNTWQALLESLKKQYNTLYGVIRMAHAEFTP
                     GHVTLTFGFVFHQKRANDPKNKKIVTSTLEEITGDRIIVDCKYDKTATPLTPTPSTSA
                     ELNAANPALGTVAAVFGGGEVLQ"
     gene            1469..>2104
                     /locus_tag="VN778_03065"
     CDS             1469..>2104
                     /locus_tag="VN778_03065"
                     /inference="COORDINATES: protein motif:HMM:NF012974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase N-terminal domain-containing
                     protein"
                     /protein_id="HXR49982.1"
                     /translation="MAGVKEETTLPYNLDAERSVLGSLLIDSDAIVKIYDKLIKEDFY
                     SQQHQYIYESVASLYDNRKAIDVLTLANELKARKHLDDAGGAAYLTELTNYVPTASHV
                     EDYTTLVVDKSVRRQLIQVNKALSGKILDETKAVKDVLGDIEAELLRISEGQIKDSVV
                     SIETILESSFERLDALHRDKKKLRGIPTGFKDLDNMIAGLQRSDLFILAARP"
BASE COUNT          571 a          608 c          529 g          396 t
ORIGIN      
        1 ctcgacgaag ttatcgggca ggaacatatt accgaaacgc tgaaacgggc gatcaagtca
       61 ggccgggtta gccacgccta tctatttacg gggccacgtg gcgtcggcaa aacgagtatc
      121 gcccgtattc tggcgcacga actgaatgaa gtcgcttacg aaggcgaaga cgcccacctg
      181 gacattattg aaatcgatgc ggccagcaac gggggcgtcg acgagatacg cgatttgcgc
      241 gacaagatct acattgcgcc cgccaatggc aaatacaaag tctacatcat cgacgaggtc
      301 cacatgatga cgacctcagc gttcaacgcg ctcctgaaga ctctcgaaga accgccggct
      361 cacgccattt ttattctcgc caccaccgaa gcacacaagc tgccggcaac gatcatcagc
      421 cgtacccagc gtttcacctt taagccgatt gcccacgata aaatggtaaa gcatctacgc
      481 acgatcgctg atactgagaa aatcgccatc gatgacgagg ctctcgacct gattgccgaa
      541 ctaagcgacg gcggcttccg cgacgctatc ggtatgctcg atcaggcgag tggcagcgcc
      601 aagaagattg atcgtgcggc catcgaggcg ctgcttggca ttcctaccga agccagcact
      661 acgcaaatac tagacctgat ccaggcacac gacgccagca gcctggcggc tcagttggca
      721 gaattacggg caaacggctt ccaggctagt gcagtcgcca aacagttggc gcaacatctt
      781 cgaagcggcc tattggccgg caagcccgca ctggaaccga tcaatctgct gacgctcctc
      841 gatcgactgg tagatgtgcc agcctcgcat aatgccgacc agctgctaga gatcgtcctg
      901 ctcgaagcag ctttgcgtgg cgcagcagca cccatgcctg aagccgcacc gagcgtcagc
      961 atagcaccgc cagctccaat acctgctccc atgccgccct ccgaacataa gccagctcag
     1021 aaaccaagcg agccggcggc aggagcggag gctgacacac caagcagcaa taaactcatc
     1081 gacctgaaca cctggcaagc actgttggaa tcgctgaaaa agcagtacaa cacgctctac
     1141 ggggttatcc gcatggcgca cgctgagttt acgcccggac acgtaaccct gacattcggg
     1201 ttcgtattcc accaaaaacg tgcgaacgac ccaaaaaata agaagattgt aacctcgaca
     1261 cttgaagaaa tcaccggtga cagaatcatc gttgactgca aatatgataa aaccgctacc
     1321 ccgctgacac cgacacccag cacttcggcc gaactaaacg cggccaatcc ggcactcggc
     1381 acggtggcag ccgtctttgg cggcggcgag gtgcttcaat aattagcatg atgcgctagc
     1441 tgtaaaattc ggatatagta tagataccat ggcaggggtt aaagaggaga cgacgctacc
     1501 gtacaaccta gatgctgaac gcagcgtcct aggttctctt ttgatcgata gtgacgcaat
     1561 cgtcaaaatc tacgacaagc ttatcaaaga agacttctac agccagcagc accagtacat
     1621 ctatgaatct gtggcctcac tgtatgacaa tcgaaaggcc atagatgtcc tgactctggc
     1681 caatgagtta aaggcacgga agcacctgga cgacgcagga ggcgcggcat acctcaccga
     1741 attgaccaac tacgttccga ccgcgtctca cgttgaagat tacacgacct tggtggttga
     1801 taaatccgtg cgccgccaac taatccaggt caataaagcc ttgagcggaa aaatcctcga
     1861 tgaaaccaag gccgttaaag atgtattagg cgatatcgag gccgaactac tccggatcag
     1921 tgaaggccag attaaggatt cagtcgtcag tatcgaaacc attctcgaat caagcttcga
     1981 acgcctcgat gcattgcacc gcgacaaaaa gaagctgcgc ggcatcccca ccggatttaa
     2041 agatctcgat aatatgatcg ctgggctgca gcgctcggac ctgttcatct tggctgctcg
     2101 gcca
//
LOCUS       DATNGP010000122         7099 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_1331213, whole genome shotgun sequence.
ACCESSION   DATNGP010000122 DATNGP010000000
VERSION     DATNGP010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 7099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7099
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1331213"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="VN778_03070"
     CDS             <1..226
                     /locus_tag="VN778_03070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49983.1"
                     /translation="DEFAREKLQLPARVGKLHNVGGLVDTVEDGAFVTVVGLMLLDML
                     LLPSLPPMHGKSGQQARNFLSGLFTRFSGS"
     gene            396..1739
                     /gene="ftsZ"
                     /locus_tag="VN778_03075"
     CDS             396..1739
                     /gene="ftsZ"
                     /locus_tag="VN778_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HXR49984.1"
                     /translation="MPQVVEPAIETFAQIKVVGVGGAGGAAINRMVDAGVENVEFIAI
                     NTDAQALHHSKADKKIHIGKDATSGLGAGADPSVGEVAAEESLDEIREAIDGADMVFV
                     TIGAGGGTGSGAGHVVAKVAGDLNALVVGFATKPFAFEGEKRRKNAESAIDKLRNAVD
                     TLIVIPNDRLLQTIDRDTPLMDAFKVADDVLRQGVQGISDLITVHGLINLDFADVKTV
                     MSKAGSALMGIGRASGENRAVAAAEQAIESPLLEVSIDGARGILFNVIGGNDLAMHEI
                     NAAAEAITAAADPEANIIFGATINPDLDGEVIITVVATGFDRSYYAKEGNGEPARPVT
                     TPVLTTATTSRQDEKALSEIDMNLSDDEDHASDFHKDTPMSNIWSLSDDEDEGDEDKA
                     SKKDEAKPEDKNKDEDSTDIVTSNLEDDLEKPSFLRRLGRRKKDADSTEDEPKGE"
     gene            2003..2482
                     /gene="nrdR"
                     /locus_tag="VN778_03080"
     CDS             2003..2482
                     /gene="nrdR"
                     /locus_tag="VN778_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003347490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HXR49985.1"
                     /translation="MKCTQCQSDDIKVIESRDVAEGQAIRRRRECVTCGYRFTTYERL
                     ERPQLIVIKNDGTRELFNRTKILAGLYRACEKTTVTSLQLEKVVDAIEQELYACGDQE
                     VKSSRIGELVMEQLARLNEVAYVRFASVYRRFTDIAGFEKELSIVREGKHIPTPVKE"
     gene            2631..5489
                     /locus_tag="VN778_03085"
     CDS             2631..5489
                     /locus_tag="VN778_03085"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020573137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HXR49986.1"
                     /translation="MPRPSGQKAGKKEGRTMGQTTSLGVRRLFTPKKSKAYDQLKWSN
                     KDSIISNPMTGTPVFEQRDVEFPEGWSLNAINIVAQKYFSGTPGTAERESSLKHLIDR
                     VVDTVTRQGVQEGYFENETEAEDFREELKYVLATQRAAFNSPVWFNIGAKGRAQQASA
                     CFILGIEDTMPAILNWYVEEGMIFKGGSGSGINISPIRSSVEALGKSAGTASGPLSFM
                     RGADSSAGAIKSGGKTRRAAKMVILNVDHPDVEEFTWSKAIEERKARDLQNLGWDMSL
                     DGKDAFSVQYQNANISVRVTDDFMQAVENDDDWDLRAVTTGKPVKTVKARKLFRQIAE
                     AAWECADPGMQFDTTINKWHTTPKAGRINGSNPCSEYMHLDNSACNLASINLLKYLRD
                     DFSFAIDEFKHTCELIFTAQEILVGYSEYPTEKITKNARAFRELGIGYANLGALLMAQ
                     GLPYDSAEGRAQAAAITALMTGHSYAVSAKLARRVGPFAGFNKDREGMLSVLKMHRSE
                     VSNIDASLVSEELLSAAATAWDEAVELGDLYGVRNSQASLLAPTGTIGLMMDCDTTGI
                     EPDLGLVKVKKLVGGGTMHIVNQTVPRALKALGYTKPQIDDIIAYIDTEKTILGAPHL
                     KKAHEPVFACSMGDNAIHYLGHVKMMAAVQPFLSGAISKTVNMPEEVTVEDVEQLHVD
                     SWKMGLKAVAIYRDNCKVAQPLSMAKKEGAAKEEPAETVEAINDKYIVKGAVRRELPK
                     VRNGKTFSFHVADSHGYMTVGEYEDGNPGEIFMRIAKQGSTLAGFMDAFAISVSHGLQ
                     FGVPLKDYVKSFTSMSFAPSGITDDPEIRTASSLVDFMFKRLALSYLSFDDRLELGLA
                     SIDDMPELQTTLLETPVAAPVAEAAPVKQEEPAKKVAETPVAASPAVSEAPARPTEEA
                     KALVGDSTAPLCYNCGNQTQRAGSCYVCSSCGSTTGCS"
     gene            5646..5912
                     /locus_tag="VN778_03090"
     CDS             5646..5912
                     /locus_tag="VN778_03090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49987.1"
                     /translation="MKVLVIYRPNSEHGRLVEEFIHEFQRRYGDSRLEILNIDTRDGS
                     ATASLYDVVQYPAILILQNDGYLQKSWEGNDLPLMNEVFAYANA"
     gene            6149..>7099
                     /locus_tag="VN778_03095"
     CDS             6149..>7099
                     /locus_tag="VN778_03095"
                     /inference="COORDINATES: protein motif:HMM:NF012361.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HXR49988.1"
                     /translation="MKFKKGTRRKAIEYEKDLVQLWKKHRTFEKSVEQRPASDVYVFY
                     DGPPFITGVPHHGTLLSSIVKDAVPRYWTMKGKRVERRWGWDTHGLPAEVFTEKKLGI
                     KDRRDIGTKISLEEYIRTCRENMIQTGSEWEDTIDRIGRWVDFRGAYKTMDKDYMESV
                     WWAFQQLYNAGKIYEGEKVLLYCVRDATPVSKAEVAMDNSYQDVTDPSVYVKLKLKDS
                     DASLLAWTTTPWTLPANTAAAVNPTIEYAEVEVDGQVFIMAKELLNKALTDEKHNVVP
                     YTIRRTFKGKELIGKSYEPLSEDRGKNAHKVWAADYVTLEE"
BASE COUNT         1840 a         1821 c         1934 g         1504 t
ORIGIN      
        1 ggacgaattt gcccgcgaaa aattgcagct gccagcccgc gtcggcaaac tgcataatgt
       61 cggcggtctg gttgatacag tcgaagacgg agcttttgtt actgtcgtcg ggcttatgct
      121 actcgatatg ttattgctac ccagcttacc gcccatgcat ggcaagagcg ggcaacaggc
      181 tcgcaacttt ttgagtgggt tatttactcg cttttctggc agctaagagc gacatatatc
      241 ggcttgcgca caaaaatgag tgccggggcc aggccgcagt gaatacttaa atgtagctga
      301 ctatactgcc actacgtccc gtataaattt gttgaacttt cagagacgct ccggtatact
      361 acgaacataa tttagcacct agggagcaaa gaattatgcc acaagtcgtt gagccagcta
      421 tcgagacatt cgcccagatt aaagttgtcg gcgtcggcgg tgccggcggc gcggccatca
      481 accgcatggt cgatgccggc gttgaaaacg tcgaattcat cgctatcaat accgatgccc
      541 aggccttgca tcattccaag gccgacaaaa agattcatat aggcaaagac gctaccagcg
      601 gcctcggtgc gggcgctgat ccatcagttg gcgaagtagc tgccgaggaa tcgctggacg
      661 aaatccggga agcaattgat ggcgcagaca tggtctttgt gactattggc gccggcggcg
      721 gtacgggcag cggcgctggt catgtcgtgg ctaaagtggc cggcgatttg aacgcgctgg
      781 tcgtcggttt tgccaccaag ccgtttgctt ttgaaggcga aaagcggcgc aagaacgccg
      841 aatccgctat cgacaaattg cgcaatgccg tcgacacttt aattgttatt ccaaatgacc
      901 gcttgctgca aaccatcgac cgcgacacgc cactcatgga tgctttcaaa gttgccgacg
      961 acgtgctgcg ccagggcgtc cagggcattt ccgacctgat taccgtccac ggtttgatca
     1021 acctggactt tgccgacgtc aaaaccgtca tgagcaaagc cggttcggcc ctcatgggta
     1081 ttggccgggc cagcggcgaa aatcgcgccg tggccgccgc cgagcaagct atcgaatcgc
     1141 cactcctaga agtctccatt gatggcgccc gcggcatctt attcaatgtc attggtggca
     1201 acgacctagc catgcatgaa atcaatgcgg ccgccgaagc tatcacggca gcagccgatc
     1261 ccgaagccaa tatcattttc ggcgccacta tcaatcccga tctcgacggc gaggttatta
     1321 ttactgtcgt cgccaccggc ttcgaccgct catattacgc caaagagggc aatggtgaac
     1381 cggcccggcc tgtcacgaca ccggtattga ccactgccac cacctctagg caagatgaaa
     1441 aagcactgtc ggaaatcgat atgaatttaa gcgatgacga agatcatgcc agcgattttc
     1501 ataaagatac gccaatgtcg aacatctggt ccttaagcga tgacgaggac gaaggtgacg
     1561 aagacaaggc aagcaaaaaa gatgaagcca agcctgaaga taagaacaag gatgaagaca
     1621 gcaccgatat tgtcaccagc aacttagaag acgacctcga aaaaccctct ttcctgcggc
     1681 gcctcggtcg gcgcaaaaag gacgcggaca gcactgaaga cgagccaaaa ggcgagtagt
     1741 cccgggctac accaggctgg cggtctgttt taaattgtac agcggatttt tgcttgcaaa
     1801 acgctacagc tagggctata ttaggaacac acagcactat atatagaaaa acgttcgata
     1861 gtgtgcgtac attaaatagc aagataaacc tcggaaaagc gcgggagaat aggtattgaa
     1921 gaatgctttt tcgcacaatt tttgtgcgca aaaccaattt ttttcggacc taccgtgctt
     1981 ggacgagtga ggaggcacta aagtgaaatg cacccaatgc cagagcgacg atatcaaagt
     2041 cattgaatcg cgcgatgttg cagagggtca agccattcgc cgccgccgtg agtgcgtcac
     2101 atgtgggtat cgtttcacca cgtatgaacg tttggaacgg ccccagctga tcgttattaa
     2161 aaacgacggc acgagggagc tgttcaatcg caccaagatt ctcgccgggc tgtatcgggc
     2221 ttgcgagaaa acgaccgtta caagcttaca gctggaaaag gtcgtcgatg ctattgagca
     2281 ggaactgtac gcttgcggag accaggaagt aaaatcgtca cggattggcg agctggtcat
     2341 ggagcaattg gcaagactca acgaagttgc gtatgtgcgc tttgccagcg tctacaggcg
     2401 cttcacggat attgcagggt tcgaaaagga gctctcgatc gtccgcgaag gcaagcatat
     2461 accaacgccg gtcaaggaat aagccaaaat tccttgacgg ctctctccgg tttcgaatat
     2521 acttaccgta ctttcggggt cgcagagagc tcatacggat ttacctgcct gagagccagg
     2581 taaagctaag aactctaaca aaaacattaa aaaacaatag taaaaacgag gtgcccaggc
     2641 cttcgggaca aaaggccggg aagaaagagg gtagaactat gggacagaca actagcttag
     2701 gcgtgaggcg acttttcacc cctaaaaaaa gcaaggcata cgaccaattg aagtggtcga
     2761 ataaagactc gattattagt aatccaatga ccggcacgcc ggtctttgag cagcgtgacg
     2821 tggagtttcc agagggttgg tcgctcaacg ccatcaatat tgtggcccaa aagtacttct
     2881 caggtacacc ggggacggcg gagcgcgaga gctctttgaa gcatcttata gatcgtgtag
     2941 tcgatactgt tacccgacag ggcgtgcaag aaggttactt tgagaatgaa accgaagccg
     3001 aagatttccg cgaagagcta aagtacgtct tggcgacgca gcgcgcggct ttcaacagtc
     3061 ccgtgtggtt caacatcggc gccaagggcc gggcgcaaca agccagcgct tgtttcatcc
     3121 tcggtatcga agacaccatg ccagccatcc tgaactggta cgtcgaagaa ggcatgattt
     3181 tcaagggcgg ttcgggttca ggcatcaaca ttagccccat ccgttcaagc gtggaagcct
     3241 tgggtaaaag tgccggcact gccagtggcc ctttgagctt tatgcgcgga gctgattcca
     3301 gtgccggggc catcaagagc ggtggcaaaa ctcgccgcgc cgccaagatg gtcatcctga
     3361 atgtcgatca ccccgatgtc gaagagttca cctggagcaa ggcgatcgag gaacgcaaag
     3421 cccgcgacct gcaaaactta ggctgggata tgagcttgga cggcaaggat gcgttcagtg
     3481 tccagtacca aaacgctaac atctctgtcc gggtgaccga cgacttcatg caagccgtcg
     3541 aaaacgacga cgattgggac ctacgcgcgg tgactaccgg caaaccagtt aagaccgtca
     3601 aggcgcgcaa gctgttccgg caaatcgccg aagcggcttg ggaatgcgcc gatcccggca
     3661 tgcagttcga taccactatt aataagtggc acactacgcc gaaagccggc cgcatcaacg
     3721 gctccaaccc gtgcagcgag tacatgcacc tcgataactc ggcctgcaat ctggcgagca
     3781 tcaacttgct gaaatacctg cgcgatgact tcagttttgc tatcgatgag ttcaaacaca
     3841 cctgcgagct gatttttacc gcccaggaaa tcctggtcgg ctacagcgag tacccaactg
     3901 agaaaatcac taagaatgcc cgcgctttcc gggaactcgg catcggctat gccaatctcg
     3961 gtgcgctgct gatggcgcaa ggtttgccat acgacagtgc cgaaggccgc gcccaggctg
     4021 ccgctatcac ggcgctgatg accggccata gttatgcggt gagcgctaag ctggcccgtc
     4081 gggtcggccc atttgccggc ttcaacaaag accgcgaagg tatgttaagc gtactaaaaa
     4141 tgcatcgcag cgaagtttct aatattgacg ccagcctggt ttccgaagag cttttgagcg
     4201 ctgcggccac tgcctgggac gaagcggtgg agctcggtga cctctatggt gtgcgcaaca
     4261 gtcaagccag cttgctagcc ccaaccggca ccatcggcct gatgatggat tgtgacacta
     4321 ctggtatcga gcctgatctc ggcctggtca aagtcaaaaa actggtcggc ggcggcacca
     4381 tgcacatcgt caaccagacg gtgccgcgcg ctttgaaggc gctcggctac accaagccac
     4441 agattgatga catcatcgcc tacatcgaca ccgaaaagac tatccttggt gcgccacacc
     4501 tcaaaaaagc tcacgaaccg gtcttcgcct gctcgatggg tgacaacgct atccattact
     4561 tgggtcacgt caagatgatg gccgccgtgc agcccttcct atccggcgct atctcgaaga
     4621 cggtcaacat gcccgaagaa gtcaccgtcg aggatgtcga gcaactgcac gttgactcct
     4681 ggaagatggg cctcaaagcc gtcgccatct accgcgacaa ctgcaaggta gcccagccgc
     4741 tcagcatggc caaaaaagag ggcgctgcca aagaagagcc ggctgagacc gtcgaagcca
     4801 tcaatgataa gtacatcgtc aaaggcgctg tccgccgcga actgccgaag gttcgcaacg
     4861 gcaaaacgtt tagcttccac gtcgccgaca gtcatggcta tatgaccgtt ggcgaatacg
     4921 aagatggcaa tccgggcgaa atcttcatgc gtatcgccaa gcagggctca acgctggccg
     4981 gcttcatgga cgctttcgca attagcgtca gccacggctt gcagttcggc gtaccgctga
     5041 aagattacgt caagtcgttc acaagcatga gcttcgcgcc aagcggtatt accgatgatc
     5101 cggaaatccg cacggcctcc agcctcgtcg acttcatgtt caagcgcctc gctttgagct
     5161 acttgagctt cgacgatcgc ctggagctcg gcttagcttc catcgatgac atgccggaac
     5221 tgcaaacgac tttgctggaa acgcccgtcg cggctcctgt tgccgaagcc gctccggtca
     5281 agcaagaaga accggccaaa aaagtcgctg aaaccccggt tgcagcttca ccggctgtct
     5341 ccgaggctcc ggcccggcca accgaagaag ccaaagcctt ggtaggtgac tcgaccgcac
     5401 cgctgtgcta caactgtggc aaccaaaccc agcgcgccgg cagctgctat gtctgctcga
     5461 gctgcggctc gaccaccggc tgcagctaat agagcaaaat tgtagtcata ataaaaaaaa
     5521 ggggggggag agcggcccct cctttattat tttcagagga aattattgag aagttattaa
     5581 attttggtta actttttggc acttcggctt gacccgggcg gtaaaggggc gctaccctta
     5641 aaggtatgaa agtgctagtg atttatcggc ctaattccga acatggacgc ctcgtcgagg
     5701 agtttatcca tgaatttcag cgccggtacg gcgactctcg cttggaaata ctgaatatcg
     5761 acacccgtga tggcagtgcc acagccagct tatatgatgt cgtacagtat ccggcgattt
     5821 taattctgca aaatgatggt tatttgcaaa aatcttggga aggcaacgat ctgccgctga
     5881 tgaacgaagt ctttgcgtat gccaatgcct gagctagggt tcagagatct gtggtataat
     5941 ttacgtacaa tttggtaaat ccgttatcag cggctatcac ccgcgaggag ccggaagaac
     6001 agcccttaaa tttttttaag aaaagcctag tagaaccagc cggagaggtc cgttatcacc
     6061 gtaagtaagt cccgaagaag caggatggta ccgttcgctc agcgagctcc tgcgtaaccc
     6121 aggggctttt tatttttaag gaggagcagt gaagttcaaa aaaggcacgc gccgcaaggc
     6181 tatagaatat gagaaagacc tggtgcagct ctggaaaaag caccgcacat ttgaaaagtc
     6241 ggtcgagcag cggccggcta gcgatgtgta tgtgttttat gatggaccgc cgttcatcac
     6301 gggcgtgccg catcatggta ctttgctctc gagtatcgtt aaggatgccg tgccacgtta
     6361 ctggactatg aaaggcaagc gggtcgagcg acggtggggc tgggataccc atggcttacc
     6421 agctgaagtc tttactgaga aaaagcttgg catcaaggat cggcgcgata tcggcaccaa
     6481 aattagctta gaagaatata tccggacttg ccgggaaaat atgattcaaa cgggcagcga
     6541 atgggaggat actattgatc gcattggtcg gtgggtggac tttcgtggcg cttacaaaac
     6601 catggataaa gattacatgg agtcggtgtg gtgggctttt cagcaattat ataatgccgg
     6661 caaaatttat gagggcgaaa aggtgctctt gtactgcgtg cgtgatgcga cgccggtcag
     6721 taaagccgaa gtggctatgg ataattctta ccaggatgtc acggatccgt cagtctacgt
     6781 gaagcttaag ttaaaagata gcgatgcttc cttgttggct tggacgacga cgccctggac
     6841 gttgccggca aatacggccg cggccgtgaa ccccaccatt gagtatgctg aggtcgaggt
     6901 cgatggtcag gtatttataa tggccaaaga attgctcaac aaggcgctaa ctgatgaaaa
     6961 acacaacgtt gtgccatata caattagacg cactttcaaa ggaaaagaac tgattgggaa
     7021 gagttatgag ccgctaagcg aagatcgtgg taaaaatgcc cacaaggtgt gggctgctga
     7081 ttatgtgacg ctcgaagag
//
LOCUS       DATNGP010000123         2982 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_2524904, whole genome shotgun sequence.
ACCESSION   DATNGP010000123 DATNGP010000000
VERSION     DATNGP010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2982)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2982)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2982
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2524904"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..995)
                     /locus_tag="VN778_03100"
     CDS             complement(<1..995)
                     /locus_tag="VN778_03100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49989.1"
                     /translation="MQQKVPLRRRILTIVSSPRFFWAIVLLLAFQASWIALSARYPMA
                     FDEDFHFGVIRIYAHHLSPFLSGQPPGADAYGAVAHDPSYLYHYLMSFPYRLISLFTD
                     NQTAQVIALRFVNIALMCSGLMVYRRLLLKVGASRAITNFSLLIFVLLPVVPLLAGQI
                     NYDNLLLPVTGLALLAAVEFVDELEDYKRINTKLLITTLILCMLASLIKYAFLPIFLA
                     IGLYFLFGLKRAFPTFRKLLVSLGFGFSLTTRSMRWFLIVAFIVSSGLFIQRYGVNVV
                     RYHTPVPDCSKVLSVKECSAYGPWIRDYNFKINKVDEEHSPFVFTADWLYGMWLR"
     gene            1065..1928
                     /locus_tag="VN778_03105"
     CDS             1065..1928
                     /locus_tag="VN778_03105"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HXR49990.1"
                     /translation="MNVRTWRPYLSILVLLATVTFFVYFFVKHPVVRAQLEHTSPAAL
                     GLLLLIYLGTVVALAMINDATLRLCKVELGASESLLLTAYSSIINFFGPLQSGPAFRA
                     VYLKKKYGLNLKNYTIATFMYYFFFACFSGLFLLSSILKGWLIVLALAGLFLALLARQ
                     NSWVKARLQALDLRSWYYLALATFLQVGLVALIYYIELKNVAPGTHFTQSLVYTGAAN
                     LALFVSLTPGAIGFREAFLLFSQRLHHISSSTIVAANIVDRAMYISLLLILALFIFGT
                     HAQRQFKVRQN"
     gene            1973..2650
                     /locus_tag="VN778_03110"
     CDS             1973..2650
                     /locus_tag="VN778_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009850066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXR49991.1"
                     /translation="MRILVIEDEPKIAQAVKRGLELKGYAVDMVHDADSGLAYATDPD
                     YDAIVLDRMLPGSLDGVALCRKLRAEEVHTPILMLTARGTIGDRVEGLNSGADDYLVK
                     PFSFDELTARVRALLRRPINQVGTNLKVEDLSLNPENYEVKRSGKPIKLSNKEFALLE
                     YFMHHPGQVITKDMIINHVWDQDADILPNTIEVYIGYLRNKIDRPFPDARPLLHTLRG
                     FGYKLSP"
     gene            2660..>2982
                     /locus_tag="VN778_03115"
     CDS             2660..>2982
                     /locus_tag="VN778_03115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49992.1"
                     /translation="MFRSATFKLTMWYMTIVMLISLAFSVALYNVATGELRRGLHRET
                     ERIYNQFPVFENNPSLPLPSTDFDSSAHRILFRLVGLNIVVLVGAGFASYWLARKTIE
                     PIEAA"
BASE COUNT          752 a          739 c          713 g          778 t
ORIGIN      
        1 aagcgtagcc acataccgta tagccaatcg gcagtaaaca caaacggact gtgctcttcg
       61 tcgaccttat taatcttaaa attataatca cggatccatg gaccgtaggc gctgcactct
      121 tttacgctca atactttgct acaatccggt acaggtgtat ggtagcgcac cacattgacg
      181 ccataacgct gtataaacaa gccgctactg acaatgaagg ctacaatcag gaaccatcgc
      241 atgctgcgtg tcgtaagact aaagccaaaa cccaaggata caagcaactt gcgaaaggtc
      301 ggaaatgccc gttttagccc aaacaggaag taaaggccaa tagccaaaaa gatgggcagg
      361 aaggcgtatt taatgagact tgccagcata cacaaaatca aggtcgttat gagtaatttc
      421 gtattgatac gtttgtaatc ttcgagttcg tcaacaaact caacggccgc caggagggct
      481 agacctgtta ctggcaacag caggttatcg tagttaatct ggccagctag aagcggcaca
      541 actggcagga ggacgaaaat cagcaacgag aagttagtga ttgcccgcga agcgcctacc
      601 ttgagcaata aacggcgata caccatgagg ccgctgcaca tcagagcaat attcacaaag
      661 cgtagtgcaa tgacctgcgc agtctgattg tcggtgaata aacttatcag gcgataggga
      721 aaactcatca gataatgata gaggtaggag gggtcatgcg ccaccgcccc ataagcgtcc
      781 gcgcccggcg gttggccgct cagaaaggga ctcaggtggt gcgcataaat gcggatgaca
      841 ccaaagtgga agtcctcgtc gaaagccatc ggatagcggg cgctgagagc aatccagcta
      901 gcctggaaag ccaggagtaa gactatggcc caaaaaaagc gtgggctact cacgattgtc
      961 agaatacgcc gccgaagtgg tactttttgc tgcatgatct cttacttatt attataccgg
     1021 caaactgtaa acgagcttaa taatgataag ctaaaggtat tgttatgaat gtacgtactt
     1081 ggcgacctta cctcagtatt ttagtcctgc tggcgaccgt gacttttttt gtctactttt
     1141 tcgttaaaca tccagtagtc cgtgctcagc tggaacacac ttcgccggcc gctcttgggt
     1201 tattattgct aatctatctg ggcaccgttg tggccttggc tatgattaac gatgctacgt
     1261 tgcggctttg taaagttgaa cttggcgcct cggaaagctt actgctaacc gcctattcgt
     1321 ctatcattaa ctttttcggg cctctccaga gcggaccggc attccgagcg gtctatttga
     1381 aaaaaaagta cggtttaaat ctcaaaaatt atacgattgc tactttcatg tactactttt
     1441 tctttgcttg ctttagcggt ctgtttttac tttcgagcat tctaaaaggc tggctcattg
     1501 tcctagcttt ggccgggctt ttccttgccc tactagcgcg tcaaaattcg tgggttaaag
     1561 cccgtttgca ggctctcgac ttacgcagct ggtactacct agcccttgcc accttcctgc
     1621 aagtgggctt ggtggcgctg atttattaca ttgagcttaa aaatgtcgca ccaggcacgc
     1681 acttcacgca aagcctggtc tacaccggtg ccgcaaattt ggcacttttc gtgtctctga
     1741 cccctggcgc cattggtttc cgcgaagcct tcttgctatt ttcccaacgc ttgcatcata
     1801 tttcaagcag tactattgtt gccgccaata ttgtcgatcg ggctatgtac atcagcctgt
     1861 tgctaatcct ggcgctattc attttcggta cacatgctca acgtcagttc aaggtacgcc
     1921 aaaactagtc ggtttacagc tatttttcat actggggctt ataatatttt taatgagaat
     1981 actggttatc gaagacgagc cgaaaatagc ccaggcggtg aaacgtggct tggagctaaa
     2041 aggctacgct gtcgacatgg tgcatgatgc cgacagcggg ttagcttacg ctaccgaccc
     2101 ggattacgat gcaattgtcc tggatcgcat gttgcctggc agcctggacg gggtcgcact
     2161 ctgtcgtaag cttcgggctg aggaggttca tacgcctatt ttgatgctga cagcccgcgg
     2221 caccatcggc gaccgggtcg aagggctcaa cagcggcgcc gacgactact tagtcaagcc
     2281 gttttccttt gatgagctaa ccgcccgcgt ccgtgctttg ctgcgtcggc cgataaacca
     2341 agtcggcacc aatttgaaag ttgaagattt gtctttaaac cctgagaatt acgaagttaa
     2401 gcgcagtggc aagcccataa agctgtccaa caaagagttt gccttgctcg aatacttcat
     2461 gcaccaccct ggccaagtta tcaccaaaga tatgattatc aatcacgttt gggaccaaga
     2521 cgccgacatt ttgccgaaca ctatcgaggt ctacatcggc tacttgcgta ataaaatcga
     2581 tcgacctttc ccagatgctc ggccactttt acatactttg cgaggtttcg gctataaatt
     2641 aagcccttaa aactacgcta tgtttcgttc tgcgaccttc aaattgacca tgtggtatat
     2701 gacaattgtc atgctcatta gcctggcatt tagcgtggcc ttgtacaacg ttgcgactgg
     2761 ggaactgaga cggggactgc accgggaaac ggagcgtatc tacaaccagt tcccggtttt
     2821 tgagaataat ccatctttac cgctgccaag tacagacttt gactccagtg ctcatcgcat
     2881 cttgtttcgg ctcgttggct taaacattgt ggtgctcgtc ggtgccggct ttgccagcta
     2941 ttggctggcg cgcaagacca tagagcccat tgaggctgcc ca
//
LOCUS       DATNGP010000124         2034 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_1531031, whole genome shotgun sequence.
ACCESSION   DATNGP010000124 DATNGP010000000
VERSION     DATNGP010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1531031"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="VN778_03120"
     CDS             complement(<1..264)
                     /locus_tag="VN778_03120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49993.1"
                     /translation="MPSSVPPCLCYYKNMKLKRDGYRRNRGGTSRVYDVTCGHCGEHV
                     AFYQKDGPGALLRMYVDRFVDRPLHGEQLICGGCQSVLGSRMVY"
     gene            263..427
                     /locus_tag="VN778_03125"
     CDS             263..427
                     /locus_tag="VN778_03125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49994.1"
                     /translation="MKLMIFIGATAGGLLGGWIGSLFDGGLGLWSLLLSTIGSLVGIW
                     LGYKIGKNYF"
     gene            complement(453..1157)
                     /locus_tag="VN778_03130"
     CDS             complement(453..1157)
                     /locus_tag="VN778_03130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49995.1"
                     /translation="MSSPNEPNNTNGTHEDASNPVDPNLQQAVRYLREMVAKYGTDEH
                     FFSDKLKELGINHVEVVLNDDNTLNARWSLQRPICVPYRYEGEPTAGAAAGSLQWILI
                     FQSGGQLSDRPLPGVNLEKGVASVHGVRLMGDYRGRTIITLSSFEDDMVPRGYAESEV
                     QEAIAAAITRLSPDAEPPLYDEDTALEEGCRYCDAHMGDIVAEMISAWAMAEGYSDDT
                     YGKFKERESHSSEHDQ"
     gene            1362..>2034
                     /locus_tag="VN778_03135"
     CDS             1362..>2034
                     /locus_tag="VN778_03135"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008765309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HXR49996.1"
                     /translation="MSKNLVIVESPAKAKTIAKYLGKDYTVKSSFGHIRDLPKKGLSI
                     DIEHAFKPDYQISPDKKKVVSELKAAAKGAEVWLASDEDREGEAIAWHLTQAMKLDPK
                     TTKRIVFHEITKPAIEHAVQNPRTIDLHLVDSYQARRVLDRLVGYEISPVLWRKVRPG
                     LSAGRVQSVAVRLIVEREREIQAFKATSSFKITAIFTIDGAELKAELASKLATIEEAK
                     QFLEAA"
BASE COUNT          506 a          555 c          487 g          486 t
ORIGIN      
        1 gtataccatc cgcgagccga gcacgctctg gcatccaccg cagattagct gctcaccatg
       61 tagcggacga tctacaaagc gatcaacata catacgcagt aacgccccag gaccgtcctt
      121 ctgataaaac gccacatgtt cgccgcaatg cccacaagta acgtcgtaca cccgtgacgt
      181 gccaccgcgg ttacggcgat agccgtcacg cttcaatttc atattcttat agtaacagag
      241 gcaaggtggt acacttgaag gcatgaagct catgattttc atcggcgcta ccgccggcgg
      301 cttactgggc ggctggattg gctcactctt cgatggcggg cttggtctct ggagcctact
      361 tctgagtact ataggttcac tcgtcggcat ctggctgggc tacaaaatcg gcaaaaacta
      421 tttttaaatg aatgaggtag tgcgttagat ttttattggt cgtgctcgga agaatgactt
      481 tctcgctctt taaacttgcc gtatgtgtca tcgctatatc cttcagccat tgcccaagcg
      541 cttatcattt ccgccacaat atcgcccata tgcgcatcgc aatacctgca gccctcctct
      601 aaggctgtat cttcatcgta cagtggtggc tcagcatcag gcgacaggcg tgtgatagcc
      661 gccgctattg cttcttgaac ctcgctttct gcatagcccc gcggcaccat atcgtcttca
      721 aacgatgaaa gcgtaataat agtcctgcct ctatagtcgc ccataagccg aacgccgtgc
      781 acagatgcaa cacctttttc caagttaacc cccggcaggg gacgatcgga cagttgacca
      841 cccgactgga atatcagtat ccattgcaaa ctgcccgcag ccgctccggc tgtaggctcg
      901 ccttcgtacc gatagggcac gcatattgga cgctgcagac tccaccttgc attaagcgta
      961 ttgtcgtcat tgagtacaac ttctacatga ttgattccca attcctttaa tttgtcggaa
     1021 aaaaagtgtt catccgtgcc gtacttggct accatttccc ttaaatatct taccgcttgc
     1081 tgaagattgg ggtccacagg gtttgatgcg tcttcatgcg taccgtttgt attgttcggt
     1141 tcgtttgggc tactcatgct caacatcata ccattgcttg gcccaagagc gagcgggaat
     1201 taagcacctt tgtctttggg gagaaatcta caatagggtt tgagaaaagt aagactagcc
     1261 taggagacag catcggccgc acacgtatcc tgccgaattt tcgtaaccca caggagtaaa
     1321 gttgcaattc gcaagcgagt cgtggtaata gttaaaggca catgtcgaaa aatttagtta
     1381 tcgttgaaag cccggcgaag gccaaaacta tcgccaaata tttgggcaaa gattataccg
     1441 tcaagagtag ttttggccat attcgtgatt tgccgaaaaa gggtctcagt atagatatag
     1501 aacatgcctt taagcccgac tatcagatca gcccggacaa gaaaaaggtc gttagcgagc
     1561 taaaagccgc cgccaagggc gctgaagttt ggctggcgag cgatgaagac cgcgaaggcg
     1621 aagccattgc ctggcatctg acccaggcca tgaagctgga ccccaagacc accaagcgca
     1681 tcgtctttca cgagatcacc aaaccggcca tcgaacatgc cgtacagaac ccacgaacca
     1741 tcgatctgca cctggtcgat tcgtatcaag cgcgacgcgt cctggacagg ctggtcggtt
     1801 acgaaatctc gccggtactg tggcgcaaag ttcgccccgg cctcagtgcc ggccgcgtcc
     1861 aatcagtcgc ggtgcgcctg attgttgagc gcgagcgcga aattcaagct ttcaaggcca
     1921 ccagcagctt caaaattacc gcaattttta ctattgacgg cgctgagctc aaggccgaac
     1981 ttgccagcaa gcttgctacc atcgaagaag ccaaacagtt cctagaagcc gcct
//
LOCUS       DATNGP010000125         4450 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_1628657, whole genome shotgun sequence.
ACCESSION   DATNGP010000125 DATNGP010000000
VERSION     DATNGP010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4450
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1628657"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..793)
                     /locus_tag="VN778_03140"
     CDS             complement(<1..793)
                     /locus_tag="VN778_03140"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXR49997.1"
                     /translation="MRISRISAQAFRGFPNEVHFSLSKAVTVIQAPNGFGKTSLSEAF
                     EWTLYGEVARKVRSKTKAEFGQEFLRSVHADQADETWAEVELIKPDGNKLVIRRTMIK
                     GGDNKLEADGKIIDSIEAVGISTGLSSRPCLGQSEIKAFIDTEPKERWKQISAILGLG
                     DFEVVREKLMKLKTDTDRDATVMQIRESARRAVAPLAPEGVDPLGQDPEALLLQLVAE
                     LSLPADTDWDVVQETAEKKITDLLSSDKKPASLDQMVTGVEEILDD"
     gene            complement(783..941)
                     /locus_tag="VN778_03145"
     CDS             complement(783..941)
                     /locus_tag="VN778_03145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR49998.1"
                     /translation="MKNQDPVLDVLEKLASKTPDALLPLIAAYRSKGIDGLDDAWKQI
                     LKDAIGEN"
     gene            1364..1612
                     /locus_tag="VN778_03150"
     CDS             1364..1612
                     /locus_tag="VN778_03150"
                     /inference="COORDINATES: protein motif:HMM:NF021452.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2164 family protein"
                     /protein_id="HXR49999.1"
                     /translation="MIRKWDLTDEQVKRQCVDEIIARIDEQGDAAFGKIAAQDIIDIV
                     ASHLGPQVFNDTIDEIKKTVEKKLADLEVDLDVLRDVS"
     gene            1809..2354
                     /locus_tag="VN778_03155"
     CDS             1809..2354
                     /locus_tag="VN778_03155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50000.1"
                     /translation="MPPYTPSKRQLERLDDKVEEAQPAIALHQSKVERHRQVSERVQR
                     LVGAYRSKGWKFLHVENEAAYDEETGLRVIYKLEGESSRYSYDKDIGKIAVIAHRPNP
                     IGRPFEDEEPVAIVRRNATFIDTAETVHWGIGITNIHSPAALDPDDNGFKSFCDTLEL
                     MEHYAAEQGILSNPAEESQNI"
     gene            2513..3382
                     /locus_tag="VN778_03160"
     CDS             2513..3382
                     /locus_tag="VN778_03160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50001.1"
                     /translation="MSGEFPSNPDQMPNRPRLSPVVKGALGILCAGAAFASGYAISSS
                     DNHGPVAPKSAPEAVTQPTKKPIKLKPTVAPSKGRSHQFNSRHHRTHPFTPQFKLKKP
                     LSPHGGEWNIAAAVALNAGNDGHAHYLRDDRRAAGIKNPYIRALAQRAVQTAEADAIV
                     FYNQGGDLGQEYPPQLSELSIRSLRSRTEAALQEDLTATIAYFAANNDPNEAQLDVNQ
                     ISSPALIAQAKLAIQQAEAGHTDEANFTWGRIENTATKDLANLNSATTNEAMILYRHT
                     DAYWQKYEQAPTH"
     gene            3482..>4450
                     /locus_tag="VN778_03165"
     CDS             3482..>4450
                     /locus_tag="VN778_03165"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXR50002.1"
                     /translation="MSRKNLALNLDAIVIGSGIQGLLLAKRLVDYGQTVAILERASTI
                     AKGSSSKNHGWLHQGTTHSLSAKDTAQAEMIARQLQYGHAFYKSYAPECFDEPILNSY
                     AVTNDANRAEYARRRWQHSNVPFKELSAEEFFRLEPDINRSAVSYFFQVADSRINNRL
                     LFSKLLTELTAKKALILTEANYEYLHDYELLVMAKQGEYTLNAPLFFYATGTGTEAAH
                     QKLMHTSLGVECFKSQLLFMPKVSRHSVIDIDYSSPIIVNHGELSAVNRSHDDVVSDG
                     TEPVVDEQEVERALSSLAALYPNAKKLRREQITAVACLKPCIPAQAN"
BASE COUNT         1248 a         1082 c          996 g         1124 t
ORIGIN      
        1 catcgtctaa gatttcttcg acgcccgtta ccatctggtc aaggctggca ggttttttgt
       61 cactgcttaa cagatccgtg attttcttct cggcagtttc ctgtactacg tcccagtctg
      121 tatcggcagg caatgataac tccgccacta actgtagaag caacgcctca gggtcttgac
      181 ctaaagggtc aacaccctca ggcgcgagtg gagcaacggc gcggcgtgca ctctctcgaa
      241 tttgcataac tgtagcatct cggtctgtgt cggtttttag cttcatgagc ttctcacgta
      301 caacttcaaa atcgccaagg cctaggatgg cactgatctg cttccaacgt tcttttggct
      361 cggtatcgat gaatgctttg atctccgact ggccgagaca tggccgcgaa gataggcccg
      421 tgctaatgcc cacggcttca atactgtcga taattttacc gtcggcttct agtttgttgt
      481 caccaccctt aatcatagtg cggcgtatta ccaatttatt gccgtctggt tttatcagct
      541 caacttctgc ccaagtctca tctgcctgat cggcgtgcac cgaacgcaag aactcttgtc
      601 cgaactcagc tttagtcttg gatcggactt tacgagctac ttcgccatat agcgtccact
      661 caaatgcttc cgataatgac gtcttgccaa acccgttagg cgcttggatc actgttacgg
      721 ctttacttaa actgaaatgg acttcgttcg gaaagccgcg gaacgcttgt gccgaaatcc
      781 tactaattct caccgatagc gtccttaagt atttgtttcc aagcgtcgtc aaggccatca
      841 atccctttac tacggtaagc tgcgattaga ggcagtaagg cgtctggagt tttgctcgcc
      901 agcttttcca aaacgtccaa taccgggtct tgatttttca tacatatatt ttactccgct
      961 aactacagta ttcatagcca tccgtatctg ttaatgctaa tgtttgcaaa aaaattttgt
     1021 gtgcgcgcta agtacaggcg agagaataac aaaagagaag gtaggggtac gtcgcaaaag
     1081 accttttacg acatacgaag tgcaacaggg tcatcgcaca atattcgaca ttgtacaacg
     1141 ccctaatctg ttgccagaat ctcagggggg gtcaagcttg caagcataga gatggagcga
     1201 aagatacggg aatctacccc tgttggctgt tcaaaagagt tgcgaagtgc gtccactagc
     1261 cggagccaag tttcaaacta agtcaacaga ggtcagtgag cctctatctt tattttgggt
     1321 aggcagtgga cggttttcta actactcttg atatgattag aatatgattc gcaaatggga
     1381 tttaactgac gagcaagtta agcgtcagtg cgttgatgaa attatagcta gaattgacga
     1441 acaaggtgac gctgcatttg gcaaaatagc agctcaggac attatcgaca ttgtagcctc
     1501 acatcttggt ccccaggttt tcaacgacac aattgatgag attaaaaaga ctgttgagaa
     1561 gaaactggct gacttagagg tcgacttaga tgtactccgg gatgtgagtt agtaaactgg
     1621 tcatttaagt tgcgaagtac gtccactagg gaggaatcgc gataatccgc ctctgttaat
     1681 tttcaggggc tatttttgtg catcaatttt taagatgtta cttggttcca attatagtac
     1741 ggttatttgt tgtttatgtt tgcggtttag ttaaaatagt attaaattag ctctaattag
     1801 gagttattat gccaccttac acacctagta agagacaact tgaaagacta gatgataaag
     1861 tcgaagaggc ccaaccagca attgctttgc atcaaagtaa agttgaacga catagacaag
     1921 tgtcagaacg tgtacagagg ttagttggtg cctaccgctc aaaaggatgg aagtttctgc
     1981 atgtcgaaaa cgaggctgcg tatgatgaag aaacgggatt gcgtgttatc tataaactag
     2041 aaggagagtc atctaggtat agctatgata aggacatagg caaaattgcc gtcatagccc
     2101 acaggccgaa ccctattggt cgtcccttcg aagatgaaga accagtagct atagtaagaa
     2161 ggaacgctac cttcatagat actgccgaaa cagttcattg gggtattggg attacaaaca
     2221 tccattcacc ggctgcctta gacccagatg ataatggctt taaaagcttc tgtgatacat
     2281 tagaattaat ggaacactac gcggcagagc agggtattct gagcaatccg gcagaggaat
     2341 cacaaaacat atgacgtata gtcgtaccaa cctctagcaa aatttaagct ttgcaataac
     2401 tttgctatcc tcaacacgat tatcaactct actttaattt ggttccattt tttcaaagta
     2461 agtcgcgcca agtttgccaa atactatttt ttatagtatt ttaaaattta cgatgtccgg
     2521 tgaatttccc tcaaatcctg atcagatgcc taataggccc cgtctttcgc cagtagtcaa
     2581 aggagcactt ggaatcttat gtgcaggtgc tgcgttcgcc tccggctacg cgatatcctc
     2641 tagcgacaac cacggacctg tcgcaccaaa atctgcgcca gaagcagtca cgcaacccac
     2701 taaaaaacca attaagctta agcctaccgt agcgccaagc aagggcagga gtcatcagtt
     2761 caactctcgc catcatcgaa ctcacccttt tacaccacaa ttcaagctca agaagccatt
     2821 gagcccccat ggtggcgagt ggaacatcgc cgctgctgtc gcacttaatg ctggcaacga
     2881 tggacatgct cactatctgc gagatgaccg tagggcagcc ggaatcaaga atccctatat
     2941 tagagcactc gctcaaagag ccgtacaaac agctgaagcg gatgctattg ttttttacaa
     3001 ccagggaggc gatctggggc aggaatatcc tcctcaactc agtgagctaa gtatacgaag
     3061 cctgagatct cggaccgaag cagcacttca ggaggacctc acggctacca ttgcctactt
     3121 cgctgcgaac aatgacccca atgaagctca attagatgtc aaccaaatat ccagcccagc
     3181 acttattgcc caggctaaat tggcaataca gcaagcggaa gcaggccaca ctgacgaagc
     3241 gaattttact tggggccgca tagaaaacac agccacaaaa gatctagcaa atctcaactc
     3301 agccacgaca aatgaagcga tgattttata tagacacacc gatgcatatt ggcaaaagta
     3361 cgaacaagcg ccaactcatt agacataaca gcaacaacaa agatgagacc aactaaagta
     3421 tttgcttttt cgtcaatatt atgtattata catattaaag aacgtaaggt atctaggtat
     3481 tatgagcagg aaaaacctgg cacttaatct cgacgccatt gtcattggct ctggcatcca
     3541 agggctgcta ctcgctaagc ggctggtgga ctacggccaa actgtcgcca tcctggagcg
     3601 ggcatccact atcgccaagg gttcctccag caaaaaccac ggctggcttc atcagggcac
     3661 cactcattcg ttatcagcca aagacacggc gcaagccgaa atgattgccc gtcagctgca
     3721 atacggacat gctttttata aaagctacgc gccggaatgt tttgacgaac caatactcaa
     3781 ttcctacgct gttacaaatg acgctaatcg ggctgaatac gccagacggc gctggcagca
     3841 cagcaatgtg cctttcaaag aactgagcgc cgaggaattc tttcggctgg agcccgacat
     3901 caaccgctcg gcagtgtcgt atttctttca ggtcgccgac agtcggatta ataaccgttt
     3961 gctcttttca aaactgctaa ctgagctgac ggccaagaaa gccctgatat taactgaagc
     4021 gaattatgaa tatttacatg attatgagct cctggttatg gccaaacagg gcgaatacac
     4081 gctaaacgcg ccccttttct tctatgccac aggtaccggg accgaagccg cccaccagaa
     4141 gttgatgcat accagcctcg gcgtagagtg ctttaagagc caattgctgt tcatgccaaa
     4201 agtaagccgt cacagcgtta ttgatatcga ctacagcagc ccgattatcg tcaatcacgg
     4261 cgagctttcg gccgtcaatc gctcccatga cgacgtagta agtgatggca ccgagccagt
     4321 ggttgatgaa caagaagtcg agcgcgccct gtcgtcactc gccgcgcttt acccaaacgc
     4381 caaaaagctg cgccgcgaac agatcacagc cgtagcctgt ctaaaacctt gcatacccgc
     4441 gcaggccaac
//
LOCUS       DATNGP010000126        18156 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_3250082, whole genome shotgun sequence.
ACCESSION   DATNGP010000126 DATNGP010000000
VERSION     DATNGP010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 18156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18156
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3250082"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..671
                     /locus_tag="VN778_03170"
     CDS             <1..671
                     /locus_tag="VN778_03170"
                     /inference="COORDINATES: protein motif:HMM:NF036802.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50003.1"
                     /translation="MLQPKFFIPIYGDFTSKRYHIDLAIEHNVPRANTLNIENGQIVA
                     LTPDKMELAGEVPHGTILVDQTGAVVSHVVVKDRVLLSEEGLVAVVLTIDKKSGNLLT
                     SPDIISRGFIYMRDNEEIMNGLRQELKRAVQQRFKRVDLDRFKAELKDHVTHYLFEHT
                     QRSPIIIPVVNVISGGGKVTQSTADQKDQPSREKTPEEVAAEQQKRFQEMRARLLTQD
                     ARID"
     gene            744..2945
                     /locus_tag="VN778_03175"
     CDS             744..2945
                     /locus_tag="VN778_03175"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HXR50004.1"
                     /translation="MAKKKKSTRKKKEEAAIEQSPLWSYVGAVVLMLAAIFLLLGGFG
                     TGGPLPVNLFHGTYWGLGWAAYLAPIALMYWGINKFSTEEHKIPRHKLFGGLAALLAM
                     AALVQVMFADKNTSGTWVGGNGGHFGYWLGAASRTVLDKVPASLLFLVFAILTTLIMF
                     GIEPQILLKLANVFKRSEGEQESELAALKAKAAEQPNFKLNEGVPVVHHGAGEQPLRS
                     GLKNTAQKLTANENHEALTTASDPNWQFPSMDLLNQKQDKADAGDVNSNAQIIHDTFE
                     NFNIEVEMEGANIGPRVTQYTLKPPTGVKLTKITALENNLALDLAATSIRMEAPIPGK
                     RAVGIEVPNVKSATVRVSSILSAQQWQETQGPLGIAVGKDISGTPIVTDLAKMPHILV
                     AGQTGSGKSVMINTILTSLLYRNSPSDLKLILVDPKRVELKPYDDIPHLLTPVIVEPE
                     KCISALKWAVAEMERRYHALSEVGKRNIIEYNSLKKEEGMPYIVIVIDELADLMMMAA
                     RDVEALIVRIAQKARAVGIHLVLATQRPSVDVITGLIKANVPARIAFTTASQVDSRTI
                     IDQVGAEKLLGQGDMLFLTADMPKPKRVQGAFIGEDETNKIADFIRMQRPPQYDDEVV
                     SQPVQLNGKGGIVADYGGQDVDDDMWRDAVQVVVDNRKASTSLLQRKLRIGYGRAARL
                     MEQMEEQGIIGQADGSRPREVLVGSVDAVFGGASAAAPEEAFTPAEAEAEI"
     gene            3046..3915
                     /locus_tag="VN778_03180"
     CDS             3046..3915
                     /locus_tag="VN778_03180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50005.1"
                     /translation="MAFEQTTPPGIQPDGLTPSKAPVSDGGFSVEPKKLKPEGLTFPI
                     DPETGLPHLVRVFYGTGDEGLITDVEHNYDHSWFPKRAPQLTDLGGKAVQLSMGQILP
                     VSIHNEKNRRFCRLSWLPKNDDEKFEAVVKGISSVVSRWAVDVRRPASNLLVRLDDAE
                     FAFLTDPKRLHRDKALKDKAANDRRDDIASFLVQHAIERNLREVITDRVINRFLNSED
                     EKYRRELGNLILKEALTARVEPFISSYSELKKKGFVRPGALPPIIAVRKFITKSQLPE
                     YYPLLTKKLLATA"
     gene            4038..4424
                     /gene="rpsF"
                     /locus_tag="VN778_03185"
     CDS             4038..4424
                     /gene="rpsF"
                     /locus_tag="VN778_03185"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HXR50006.1"
                     /translation="MQQYEIAVLYHPDLEVDLTKAEDRVKKIFTDNAGKVTATDNWGK
                     RKLAYAIKRNEHAVYVFYTVELPAEAVQKVESALNITDEVIRFLITRPDLKAIAKAEK
                     AKAEKAEQAAKRGESADSSEEAEAES"
     gene            4543..5025
                     /gene="ssb"
                     /locus_tag="VN778_03190"
     CDS             4543..5025
                     /gene="ssb"
                     /locus_tag="VN778_03190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HXR50007.1"
                     /translation="MARSLNQVTLMGNLTRDPELRQTPTGQNVTSFSLALNRSYKDQS
                     GEWQEATDYIDIVCWGPLAERVAQYLSKGRRCLVQGRLQSRSWEQDGQKRSKVEVLAN
                     DVTFLDSRSGGDSEGGYGGGAPAEAAAAKPTPGKKVKKDDVVIEDIGDEPINLDDIPF
                     "
     gene            5048..5437
                     /locus_tag="VN778_03195"
     CDS             5048..5437
                     /locus_tag="VN778_03195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50008.1"
                     /translation="MEHRVAELIDSGAFDGLILERPFDMTSITGLNEAGYGDHERQFT
                     LDQVVRFAAKAYRHFGGISYQLPPPVGFEEVLAFASDPTRQDQYLVWGHEEAVAQLAH
                     TYMVGNGADYETFLKFEEQGVINGKDF"
     gene            5421..5624
                     /gene="rpsR"
                     /locus_tag="VN778_03200"
     CDS             5421..5624
                     /gene="rpsR"
                     /locus_tag="VN778_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010131239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HXR50009.1"
                     /translation="MAKIFKHRTDVAYFDYKDFKTLQRYVNVYGQIETRKKTGLTESQ
                     QRRLAQAIKRARHIALLPFVANN"
     gene            5633..6199
                     /gene="efp"
                     /locus_tag="VN778_03205"
     CDS             5633..6199
                     /gene="efp"
                     /locus_tag="VN778_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010131241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HXR50010.1"
                     /translation="MALSITDLKKGTLFQWEGEPFRVVEYSQKVMGRGGSIVNVRIKS
                     LLDGKVLEKTFKGNEQLDSADVTNQTVQYLYSDGTIFHFMNEETFEQFEVPADLVGDG
                     AGYLKEGDRVQLQFFNDRPINVELPKNVPLKVTYTETAVKGDTSSAITKDATLETGIT
                     IKVPAFIKQGDLISVDTSTGAYRERVKE"
     gene            6202..6666
                     /locus_tag="VN778_03210"
     CDS             6202..6666
                     /locus_tag="VN778_03210"
                     /inference="COORDINATES: protein motif:HMM:NF024952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HXR50011.1"
                     /translation="MQSGDRLICAFVEPQAPGTQFKDWFLHITIVPWFRLDMPSSQLA
                     EALKKNYIGSQPFEVKVENETHFGYKKRKLVNLVSATELMKLEGQTRRLLHAHKAWVV
                     DEADKTRRRPYLPHITALTAGKVQEGSHFGVDKLCIIEQKGAYKEIMGEMVL"
     gene            6663..7382
                     /locus_tag="VN778_03215"
     CDS             6663..7382
                     /locus_tag="VN778_03215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HXR50012.1"
                     /translation="MKTRLDQKLVQNGLVSTRSQAESYVKLGEVTVNQKVITKPGFLT
                     SDTDQVMLNVKEQYVSRAALKLASVAEALKLDFINKTVLDVGSSTGGFTDYALQHGAK
                     KVIAVDVGSDQLHPSLRENPHIELHEKTDIRDFEVSEPIDLIVIDVSFISLREILPAV
                     AKLSSKRTLVAAMVKPQFEAGREKLKHKGVIKNDRLRREILKNFEQWAGQHFVVLSKA
                     DSDIKGAKGNQERFYLLTLLS"
     gene            7404..8060
                     /locus_tag="VN778_03220"
     CDS             7404..8060
                     /locus_tag="VN778_03220"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HXR50013.1"
                     /translation="MYFASRAQAGRMLARQIAKKYSHESCAIVALNDGGVVVGAQIAV
                     QLHAVLTLLVSADIDLPQEPAALASITPSGTWAYNQQYTQGEISELASEFRGFVEQEK
                     LTRMHELNAHLGVSNTIKSNLLTDRTVILVADGLETGTLLDLAYEFLKPIKLKRTVVA
                     TPLATVPAVDRMHVMADDLFCLSVVGDYMETDHYYDRKDVPDHETVVKVIEQLIQKWK
                     "
     gene            complement(8064..8723)
                     /locus_tag="VN778_03225"
     CDS             complement(8064..8723)
                     /locus_tag="VN778_03225"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HXR50014.1"
                     /translation="MLATLKQTAELVDFFRSKGLKQAFKKGDFIIRPGDSPPGVFYIY
                     EGLVKAYDITKYHEENLLIIRKADELFPLIWAITGQERHVIYQALAPTLTWQISRPQF
                     LQFVESKPDTLAPLLDMTLEMYRLHSERILNLEYRTVRERIISFLLTMSQRFGKQTPD
                     GLLIEAPLRHQDIASSVNATRETTSRELAALERKDLLTNSQTLILLKDVKALKQALGH
                     E"
     gene            8871..9866
                     /locus_tag="VN778_03230"
     CDS             8871..9866
                     /locus_tag="VN778_03230"
                     /inference="COORDINATES: protein motif:HMM:NF025825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HXR50015.1"
                     /translation="MVSPESVEKQLKKLDFNPKGWGRSEVNELPHILVPDEEIYELAN
                     GYYEAGFALLVSTNIRVVLIDRKPMNYLTVEDLRFDMINEIDYSHRLIGAVITITTGS
                     KVLKFTSYNQKRLRKLIGHVQSCMAQIKMKQSEHQEGQVSHLEKINEQLQSYLFAQQQ
                     YQQQLHQQLQQVQAGGQSATPIQPPEQVRPGPELADYLYARSLLDWHEQRQTPADAAA
                     PAPKMAVPSEQPPPPAPPAGGDRNNPQLDDLYNEAVREIFGRYHPQDEKKEPLTANAS
                     DAASASSSRFSLPLDPLRIAYSKLPMALRNRRFGRPSFHAHSQQPTPAPLPHAPR"
     gene            complement(9873..10958)
                     /gene="ychF"
                     /locus_tag="VN778_03235"
     CDS             complement(9873..10958)
                     /gene="ychF"
                     /locus_tag="VN778_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017556581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HXR50016.1"
                     /translation="MSSLSIGIVGLPNVGKSTLFNALTGSNILAANYPFATIEPNTGV
                     VPVLDPRLQKLAELYHTDKIVPATVTFVDIAGLVAGASEGEGLGNQFLSHIRSTNAIA
                     QIVRAFEDPNVTHVDEAHDPKKDIDVINTELVLADLQTLSRRLPKLEKEARANPKLKP
                     LVDTYQQVNTFLNDGEPLWNHPEIDAEAVADLQLLTMKPIIYVFNLDEKGLDDEARRQ
                     QLCEMVAPARALFVCAKLESELKDLEARDAAELLASYGQQESGLLQLIHAAYDTLGLQ
                     SYLTAGEKEVRAWTIPQGATAPQAAGVIHGDFERGFIAGEVVNYPDLIAAGSLSAAKA
                     NGKVATVGKTYVMQPDDVVEFRFNVSK"
     gene            11040..11930
                     /locus_tag="VN778_03240"
     CDS             11040..11930
                     /locus_tag="VN778_03240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50017.1"
                     /translation="MPEIVKQPHTSTEQVKQWLNHDLLLLAGGAEIVNGQLDPTVVQK
                     EHMAAEMKASLEAKQQRHIEILGDLAVKHAGPDTEEALLQRDVAEKSQMKERIDHWLD
                     TGVIKLTTWLGRNRIAREYNPRQYSNAFTDLQNKPGAALIKARRMPFAKELLAMGVSE
                     VVTVSNPQQKFGRFDRKSPQADEDAVQIDYKTLTGETAKGSPYKYRSKVVSGNSLSVG
                     IVLPKTEADQFVQAVHDDPQVMRELVEALMAERFDAGESWENARPSYEDWRNVNEGVN
                     RIAFRPTPTTYPAQSEALTF"
     gene            11991..13904
                     /locus_tag="VN778_03245"
     CDS             11991..13904
                     /locus_tag="VN778_03245"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXR50018.1"
                     /translation="MQQDQVRLNAAIEEGVSKGVPVDFIAYSLTRAGWPQDMVKEALD
                     TWLLENGRRQKTTGFTEWLRKYYAQAKPAIILMVVLNTIASAIALLQPWPLKILADSV
                     FGSVPAPGVLRPYTHTPKLILIVSLLTLLIFLLGQGFGVLKDFLLLKIDYWLNRSIKE
                     EAFRHILHLPLYHEGRLPKGDYIYRQNEVTNSLSDLVLNTTSEMIGSFILIIGVTAIM
                     LTLNAKLTLITLFVIPFLIFSVGVFGPIMGRLGQALTKLFSQTSALIAESIDNSEAVQ
                     AFTLEERQVQKLRSLWMQTYVVSRRGLLWGKAFNFSNGLVVILGTSLVIYFGGTEALR
                     GQFSLGSLLIFMTYLGYLINPIQDITAQITIRRQKLVNVRRVYEVLSDHEGIERLRAD
                     RPLPRVTGGVKFQNVTYVSGGVKVLDQVNLDIAPGQKIGIIGPSGSGKTTLLRLLDLY
                     IEPTAGRILLDGHDIQSVSLHDLRRNIAWISQTPQLFATSIAENMQDGDIERVMTPEE
                     FSWAAQAANITEFVNRLPMGMNSVAGEGGSSLSGGQKQRISIARALLKNAPIICMDEP
                     TSALDDKSEKLIHDSIGSLIQGKTVLLATHRLALLGLMDKVYVVQGGHLVDVNELGGV
                     ERYTYQLQVGGQL"
     gene            13973..15142
                     /locus_tag="VN778_03250"
     CDS             13973..15142
                     /locus_tag="VN778_03250"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HXR50019.1"
                     /translation="MTPEEEKLLAESITLHTDARNAGARKMYDDRTGNKVAAANDARI
                     QGIDNALAAPIDAQLKALESAPVPIAATPAAPAAPKPVPVADAWGSSTPTAWGQPIAK
                     PSTPKPVTHPSGGAPVDPAPPAHKPAPVADGWGSSKPTAWGEPINKPATPKPDTHPTG
                     AGSPTIHDGWGSSTPTAWGEPTAKPSTPAADKPPVATGFGTGGDVHPIHNPLSGGSAT
                     GHTNAPTASPPVPAGHSTSEGASTHTAAPASHSDGGAPVEGTASYSVVKGDTLSGIAA
                     AHHMSLSEVESLNPQIKNPNLILPGETVHLSASMPTSSSYSGISSSGSGPAQMVPGVT
                     DTSTSNKRGILGSLLGRFGKGGNTDGGVPPATDPAKTVAVDPTKSQKTEPTKPLS"
     gene            complement(15149..15742)
                     /locus_tag="VN778_03255"
     CDS             complement(15149..15742)
                     /locus_tag="VN778_03255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50020.1"
                     /translation="MIRAVFFDFYGVWTPDVFAQYLQLAEQYGTEVAGPLNVLVSKYF
                     HGMSTPEEVANTFRFKLSRPEIDAAEFTLNENSISPALIDFMRSLHGHFLKLGVLANL
                     GTQEYALLSGLNNRYQLFEVIAGPLPFTIGEPLLSQSVFAMALQQIGEPPRNCLVVSG
                     NPAYRQFAASLGMTALPFEGFPRLRESLEQLLANDVA"
     gene            complement(15784..18000)
                     /locus_tag="VN778_03260"
     CDS             complement(15784..18000)
                     /locus_tag="VN778_03260"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HXR50021.1"
                     /translation="MFEKIARFSVRFRWFIIVFWIVAIPVVTANFPNINNVTKNNESD
                     FLPKNSPTDTASKLETAFQNKKTATNAIIVVSSTSGPLTSSDTPALQRVVTAVKHVPS
                     VTEVRDSGTSADGQVHQYLVGISSDAFGQKATDIVKNIRSAIKQSGVPSGLSAHLTGD
                     LAAGVDQENANQKGRNSTEGYTVILILVLLLVVFRALLAPIVTLVPAGIALAVAQPVI
                     AESTKIGVQVGFITQILLIVLLLGAGTDYGLFLVFRVREELRGGLEPKEAVVKALSKV
                     GESITFSAATVAAALLSLLLASFGLYKGLGPALAIGLAVMLLIALTFLPALLAILGRA
                     VFWPSKTSKRKLKIGLWGRLADNVIKKPIMMLVLGALVFGGLSLGIVGYKSAGFGDQG
                     SAPSGSDSAAGQQILQQHFPAANNNPQLLIFHFPASVWNNLSPVATVENKLAASKTFT
                     AVAGPFNANGFRLTPQQLAQLHQSGTSPALQAINQFISPDGKTVQFYGVLKAGVTGSS
                     AATAAIPTVRHELQTIAAGVGADQSQIYSSDSVGYDVNRIATSDLEKIIPVVLLIIAA
                     LLALLLRSLVAPWYLILTVGLSYLASLGFAMIVFVHIAHQDGLNFVLPFLMFIFGMAL
                     GEDYNILVMSRIREEAHDHKSLSEAVTKAVGITGTTVTSAGLILAGTFTILGIVGGNE
                     QVQQIGFGIAFGILLDTFFVRTLLVPSIVALLGRANWWPSALSRPLQSQRSQTKRP"
BASE COUNT         4638 a         4639 c         4929 g         3950 t
ORIGIN      
        1 agatgctgca gcctaaattc ttcatcccga tttacggtga ctttacctct aagcgctacc
       61 atatcgatct ggcgatcgag cacaacgtgc ctcgcgcgaa cacgctaaac attgaaaacg
      121 gccaaatcgt ggcgctaact cccgataaaa tggagctcgc cggcgaagtg ccgcatggca
      181 ctatattggt ggatcagacg ggtgccgtgg tgagtcatgt cgtcgtcaag gaccgcgtac
      241 tgctgagcga ggaaggtttg gtggccgtgg tgctgactat agataagaag agcggcaatc
      301 tgctgaccag cccggacatc attagccgtg gcttcatata tatgcgtgac aacgaagaga
      361 tcatgaacgg cctgcgccag gaattaaagc gtgccgtgca gcaacgcttc aagcgtgttg
      421 atctggatcg cttcaaagcc gagctcaaag accacgttac gcactacttg ttcgagcaca
      481 cccagcgcag cccgatcatt attccggtcg tcaacgtcat tagcgggggc ggcaaagtca
      541 ctcagtcaac ggccgaccag aaggatcagc cgagtaggga aaagaccccg gaagaagttg
      601 ctgccgaaca gcaaaaacgc ttccaagaaa tgcgcgcccg cttgctgacc caagacgccc
      661 gtattgacta gggttgtaaa atttgctata atattacgcg gtaagcaatc taagctgctt
      721 aagctgatat aataagtagt ccaatggcaa aaaagaaaaa gtccacacga aagaaaaaag
      781 aagaagcggc catcgagcag tccccgctgt ggtcgtatgt cggcgcggtt gtgctcatgc
      841 tggcagcgat tttcttgctg ctcggcgggt ttgggacggg cgggccactg ccggtcaacc
      901 tgttccacgg cacttactgg ggcctgggct gggcggcata tctcgccccg attgcgctga
      961 tgtattgggg tatcaacaag ttcagcaccg aagagcacaa aatcccgcgg cataaactat
     1021 ttggtggcct ggcagcgctc ctggcgatgg cagcgctagt ccaagttatg tttgccgata
     1081 agaacaccag tggcacctgg gtgggcggca atggcggcca ttttggatac tggttaggag
     1141 ccgcctcgcg gacggtgctg gataaagtcc cggcctcgtt gctcttccta gttttcgcta
     1201 ttctcacaac gcttatcatg ttcggcatcg agccgcaaat tttattgaag ctagctaacg
     1261 tgttcaagcg gtcagaaggt gagcaggagt ccgagctggc ggcgctgaaa gctaaggcgg
     1321 cggagcagcc gaactttaag ctgaacgaag gtgtgccagt ggtgcaccac ggcgctggcg
     1381 aacagccgct tcgcagcggt ctcaagaaca cagcccagaa actgacggct aacgagaatc
     1441 acgaggccct aaccaccgct agcgatccga attggcagtt tccgagcatg gatttgctca
     1501 atcagaaaca agacaaggct gatgccggtg acgttaatag caatgctcaa attattcatg
     1561 acacctttga gaacttcaac atcgaggtgg agatggaagg cgccaatatc gggccgcgcg
     1621 ttacgcagta taccttgaaa ccaccgacag gggtgaagct gactaaaatc acagcgttag
     1681 agaataactt ggcactggat ctggcggcta cctcgattcg catggaggcg ccgatccctg
     1741 gtaaacgagc cgtcgggatc gaagtgccaa atgtcaaatc ggccaccgtc cgtgttagct
     1801 caatcctctc ggcccagcag tggcaagaaa cccagggtcc gctgggcatt gccgttggta
     1861 aagatatctc cggtacgcca atcgtgactg acctggcgaa aatgccgcat atactggtcg
     1921 ccggtcagac tggttcgggt aaatcagtga tgatcaatac cattttgacg agtctgctgt
     1981 accgcaattc gccgtcagat ctaaagctta ttttggttga ccccaagcgc gtcgaactga
     2041 aaccgtatga tgacattcca catctgttaa cgccggtgat cgtggaaccc gagaagtgca
     2101 ttagcgccct gaaatgggcg gtcgccgaaa tggagcggcg ctaccacgcg cttagcgagg
     2161 tcggcaagcg caacatcatc gagtataaca gcttgaaaaa agaagagggc atgccctaca
     2221 tcgttatcgt tatcgacgag cttgcggacc tgatgatgat ggcggcccgc gatgtagagg
     2281 cgttgatcgt gcggattgct caaaaagccc gtgctgtggg catccatctg gtgctggcca
     2341 cgcagcgtcc ctcagtagat gttattaccg gcctcatcaa ggctaacgtg ccggcccgta
     2401 tcgccttcac aaccgccagc caagtcgatt cacgcaccat tatcgaccaa gtcggcgccg
     2461 agaaattgct cggccagggc gatatgttat tcctgacggc ggatatgccg aagccgaagc
     2521 gcgtccaggg tgccttcatc ggcgaagacg aaaccaacaa gattgcggat ttcatccgta
     2581 tgcagcgccc gccgcagtat gacgacgagg tcgtgtcgca gcccgtccaa ctgaacggca
     2641 aaggcggcat tgtggccgat tatggcgggc aggatgttga tgatgatatg tggcgtgacg
     2701 ccgtacaagt ggtggtggac aatcgcaagg ctagtaccag cttattgcag cgtaaactac
     2761 gcattggcta tggccgggcc gcccgcctta tggagcaaat ggaagagcaa ggaatcatcg
     2821 gtcaggccga tggctcccgt ccgcgcgagg tgctcgtggg ttctgtcgac gccgtcttcg
     2881 gcggcgccag cgcggcagct ccggaagagg ctttcactcc tgccgaagct gaagccgaaa
     2941 tctagcccca gtcgttatcc gagggtgtgt aaaagtactt gccagcgagc gttaaatttt
     3001 ttaccataag cgtaagacaa acaactgcaa acggagggaa gcgcagtggc attcgagcaa
     3061 acgactccac caggaatcca gcccgacggc ctcacgccct cgaaagcccc tgtctctgac
     3121 gggggctttt cagttgagcc gaaaaagcta aagcctgaag gactaacctt tcctattgat
     3181 ccagaaactg gtttgccgca cttagtcagg gtgttttatg gtaccggcga tgaaggctta
     3241 ataacggatg tcgagcacaa ttatgaccat agctggttcc ctaaaagagc ccctcagctt
     3301 acagatttag gtggcaaagc cgtccagctt tctatgggac aaatcctacc tgtgagtatt
     3361 cataacgaaa agaatcggcg gttttgtaga ttatcctggt tgcccaaaaa tgatgatgag
     3421 aagtttgaag cagttgtcaa aggaatttcc agtgtagtgt cacgatgggc cgtagatgta
     3481 agacgcccgg caagtaattt gcttgtgcgt ttggacgacg cggaatttgc ctttttaact
     3541 gatccgaagc gtttacatcg agataaagct ctaaaagata aggctgcgaa cgaccgtcgt
     3601 gacgacatag cctcatttct tgtccagcat gcaattgagc gcaacctaag agaagtgatt
     3661 acagatagag taattaatcg atttctaaac tcagaggatg aaaagtaccg aagagaatta
     3721 ggtaatttaa tacttaaaga agctttaaca gcgagagttg agccatttat ttcctcttac
     3781 tccgaactga agaaaaaggg ctttgtccgg ccaggcgctc tacctcctat tattgcggta
     3841 agaaaattta taacaaagag ccagctgccg gagtattacc ctttactgac aaaaaaattg
     3901 ctcgctacag cctagcttga ccaacagggg tggataagat ataatgagct gacaatagta
     3961 acttcagctc atgactactt aggtatatgg gctgtccagg gcgaaagctc tggtaaccga
     4021 aggaaggagc accaaaagtg cagcagtacg aaattgccgt gctgtatcat cctgatctgg
     4081 aagttgacct gacaaaggcc gaggaccggg ttaagaagat cttcaccgac aatgctggta
     4141 aggtgacggc aaccgacaat tggggtaagc gcaagctggc gtacgctatc aagcgcaacg
     4201 agcacgccgt gtacgttttc tacaccgtgg aattgccggc tgaagcagtc caaaaggtag
     4261 agagcgcgct gaacatcacc gatgaagtca ttcgcttcct catcacgcga ccggacctga
     4321 aggccatcgc caaggccgaa aaagccaagg ccgaaaaagc cgagcaggct gcgaagcgag
     4381 gtgagagcgc tgacagcagc gaagaagccg aagccgaatc ttaatttttc cttaaatatg
     4441 catattttta aggagctcac gcttgacggc ggctgagcct cgggcgtagg gtaggggtac
     4501 aaattaacga agacataggt cttttaagaa ggaggtgcta agatggcacg aagtctcaac
     4561 caagtaacat taatgggtaa tctgacgcgg gatccggagc tacggcaaac gccaaccgga
     4621 caaaacgtta ccagcttcag tctggcgctg aaccgctcat ataaagacca gagcggcgag
     4681 tggcaggaag ctactgacta cattgacatc gtatgttggg ggccgctagc tgagcgcgtg
     4741 gcacagtatt tgagcaaagg ccgccgctgc ctggtccagg gtcgtttaca gtcccgttct
     4801 tgggagcagg atggccagaa gcgcagcaaa gtagaagttt tggccaatga cgtgacattt
     4861 ttagatagtc gtagtggtgg cgacagcgag ggtggctacg gtggcggtgc accagcagaa
     4921 gcagctgcgg ccaaaccgac acctggtaag aaagttaaaa aagacgatgt cgtcatcgaa
     4981 gatatcggcg acgagccaat caacctcgac gacatcccgt tttaggagat agaacttgag
     5041 aacagcaatg gaacacaggg tggctgagct aatagattca ggcgcttttg acgggctgat
     5101 tttagagcgt ccttttgata tgacaagcat taccggacta aatgaagccg gatacggtga
     5161 tcatgagagg cagtttacgc ttgatcaagt ggttaggttt gcggctaaag cctaccgtca
     5221 cttcgggggt atttcctatc agctgccacc acctgtgggc tttgaggaag tgctagcatt
     5281 tgcttcggat cccacccgcc aagatcaata tctagtttgg ggacacgaag aagcggtagc
     5341 ccagcttgca catacctata tggtgggaaa cggtgctgac tacgagacat ttttaaagtt
     5401 tgaagagcaa ggagtaatta atggcaaaga tttttaaaca tcggacggac gttgcctatt
     5461 tcgactacaa ggatttcaaa accttgcagc ggtatgtgaa tgtctatggg caaatcgaga
     5521 cgcgcaagaa gaccggttta accgaatcgc agcagcgccg cttagcgcaa gctatcaaac
     5581 gggcccggca catcgctttg ctgccgtttg tcgcaaacaa ttaggagtaa acatggcttt
     5641 aagtattact gacttgaaga aggggacgct tttccaatgg gaaggcgaac cgtttcgcgt
     5701 tgttgaatac agtcaaaaag tgatgggccg cggcggttcg atcgtcaatg tccgcatcaa
     5761 aagtttgctc gacggcaagg tgctcgaaaa gacgttcaag ggcaatgaac aactcgattc
     5821 agcggacgtc acgaatcaga ccgtgcaata cctctatagc gacggcacca tcttccattt
     5881 catgaacgaa gaaacgtttg agcagtttga ggtgccggct gacttggtcg gcgacggcgc
     5941 aggctatctc aaagaaggcg atcgcgtaca actacagttt ttcaacgatc ggccaatcaa
     6001 tgtggagctt cccaaaaatg ttccgctcaa agtcacttat accgagaccg ctgtaaaggg
     6061 cgacacgagt tcagcgatca ctaaggatgc aaccctcgaa actggcatta cgattaaggt
     6121 gccggctttc atcaagcaag gcgatcttat ctctgtcgac accagtaccg gcgcttaccg
     6181 cgaacgcgtt aaggaataag catgcagtcg ggcgatcgtt tgatctgcgc ctttgtcgag
     6241 ccgcaggcgc cgggaacaca gttcaaagat tggtttctgc acattacaat tgtgccgtgg
     6301 ttccgtctcg acatgccatc ttctcaatta gctgaagcgc tcaagaagaa ctatatcggt
     6361 agccagcctt ttgaagtcaa ggtcgagaat gaaacgcatt tcggttataa gaaacgcaag
     6421 ctagttaatc tggtaagcgc gacggaacta atgaaattag aaggccaaac tcggcgtctt
     6481 cttcacgcgc acaaagcctg ggtggtcgac gaagctgata aaacgcgccg ccggccctac
     6541 ctgccacaca taaccgcatt gacggccgga aaagtgcagg agggtagtca tttcggggtt
     6601 gataagctat gtatcattga acaaaagggc gcctataaag aaatcatggg agaaatggtt
     6661 ctatgaaaac ccgcttagac cagaaattag ttcaaaatgg actggtgtcc acccgaagcc
     6721 aggccgaaag ctacgtaaag ttgggggagg taaccgttaa tcaaaaggtg ataactaaac
     6781 ctggttttct gacctcagac accgaccagg tcatgctaaa cgtcaaagaa caatatgtca
     6841 gccgggcagc gctcaaatta gcctctgtgg ccgaagcgct caagctggac ttcatcaata
     6901 aaaccgtgct cgacgtcggc agcagcacgg gcggcttcac cgattacgcc ctacaacacg
     6961 gtgctaaaaa agttattgct gtggatgttg gcagcgatca gctgcaccca agtttgcgcg
     7021 agaacccgca catagaatta cacgaaaaaa cggatatccg cgatttcgaa gtaagcgagc
     7081 cgattgatct aatcgtcatc gacgtctcct tcatttcgct cagagagatt ctaccggccg
     7141 tggccaagct aagctccaag cgaacattgg tcgccgctat ggtcaagccg caattcgaag
     7201 ccgggcgcga gaagctcaaa cacaaaggcg tgattaaaaa tgaccggcta cggcgcgaaa
     7261 ttctaaagaa ctttgagcag tgggcgggcc agcattttgt agtgctgagc aaagccgatt
     7321 ctgatatcaa gggcgccaaa ggcaatcaag agcgatttta tctgcttacg cttttgagct
     7381 aaattcggtt aaacttaaag gatatgtatt tcgcctcacg tgcgcaggct ggacgaatgt
     7441 tggccagaca aattgccaaa aagtacagcc atgagtcttg tgccatcgtg gcactcaatg
     7501 atggcggtgt tgtggtgggt gcccagattg ctgtgcagct ccacgccgtg ctgacgctac
     7561 tggtgagcgc cgacattgac ttgccgcaag agccggcggc gctagcaagc atcacaccga
     7621 gcgggacttg ggcatacaat cagcaataca cccaagggga aatcagtgaa ttggcatcag
     7681 agtttcgggg atttgtcgag caggaaaagc taacccgaat gcacgaatta aatgcgcatc
     7741 tcggggtctc caacaccatc aaaagcaatc tcctgacaga ccgcacggta atactggtgg
     7801 ccgacggtct ggaaacgggt actttgcttg acctggcgta cgagttttta aagccgataa
     7861 agttgaaaag aacagtggta gcaacgccgc tggccaccgt accggcggtt gaccggatgc
     7921 atgtaatggc cgatgatctt ttttgtctca gtgtcgtcgg cgattacatg gaaaccgatc
     7981 actactatga tcgcaaagac gtgccggacc atgaaacggt cgtcaaagtg atcgagcagc
     8041 tgattcagaa gtggaagtag cggctattcg tggcctaaag cttgtttcaa ggctttgaca
     8101 tctttgagca aaattagcgt ctgcgaattg gtcaacaggt ctttgcgctc caaggcggcg
     8161 agttctcggc tggtagtttc gcgagtggca ttgacggagc tggcaatgtc ttggtgcctc
     8221 aatggtgctt cgatgagtaa gccatcgggg gtttgcttgc cgaagcgctg actcatcgtc
     8281 agcaggaagg agatgatgcg ctcgcgtacg gtacggtact ccaggttcag gattcgttca
     8341 ctgtgcaggc ggtacatctc tagcgtcata tcgagcaatg gggccagcgt atcgggtttg
     8401 ctctcgacga attgcaaaaa ctgcggccgg ctaatctgcc acgttagcgt cggcgccaga
     8461 gcttggtaaa taacatgacg ctcctggcca gttatggccc agatgagagg gaagagctcg
     8521 tcggctttgc ggatgatcag caagttctct tcgtgatact tcgttatgtc gtatgctttt
     8581 accaatcctt cgtatatata aaaaacaccc ggcggagagt cgccggggcg gatgatgaaa
     8641 tcacctttct taaaggcctg tttcaggcct tttgatctga agaagtctac caactcagcc
     8701 gtttgcttaa gcgtggctaa catatattaa ttatgcgacg gcacggccaa aaagtcaaac
     8761 ctggacttcg gtaggtttgt gatgaaaatc tcatgcggag cgtgagtatt ttcaccaatt
     8821 tctcagcggc agaggcgtat gatagtccta caaagaaagg gagagctaaa atggtgagcc
     8881 ctgaatcagt agaaaaacaa ctaaaaaaac tcgattttaa tccaaagggc tgggggagga
     8941 gtgaggtaaa tgaattgccc cacatcctgg tacctgatga agagatctat gagctggcga
     9001 atggttatta tgaggcaggt tttgccctgc tagtatcgac gaatattcgc gtggtactca
     9061 tcgatcgtaa acctatgaac tatttgacgg tcgaagatct caggttcgac atgatcaacg
     9121 agatagacta cagtcaccgc ctgatcggcg ccgtgataac catcacgacg ggcagtaaag
     9181 ttctgaagtt cacgagctac aatcaaaaac gtttacgaaa actgattggc cacgttcaga
     9241 gctgcatggc ccagatcaag atgaagcagt cagagcatca ggaagggcag gtatcgcacc
     9301 tcgagaaaat taacgagcag ctacaatcgt atttattcgc ccagcagcaa taccagcagc
     9361 agctccatca gcagcttcag caagtccaag ctggagggca gtccgctacc ccgatccagc
     9421 cgccggaaca agtgcgcccc ggcccagaac tagccgacta cttgtacgcc cgcagtttac
     9481 tggactggca tgagcagcgg cagacgcccg ctgacgccgc tgccccggct ccgaaaatgg
     9541 cagtcccgtc agagcaacct ccgccacccg ctccgcctgc gggaggcgat cgtaataacc
     9601 cgcagctcga tgacttgtac aatgaagcgg tacgcgagat ttttggacgc tatcaccccc
     9661 aggatgagaa aaaagaaccc cttaccgcta acgccagtga tgctgcctct gcgagcagct
     9721 cccgcttttc gctccccttg gatccactgc gcatcgctta ctcaaagctg ccaatggcgc
     9781 tcaggaaccg ccggttcgga aggccttcgt tccatgcgca cagccaacaa ccgacccccg
     9841 cgccactgcc gcacgcaccc cggtaaatgc ggttatttgc taacgttgaa acgaaactcg
     9901 acgacgtcgt caggctgcat gacgtaggtt ttgccaacgg ttgcgacctt gccattcgct
     9961 tttgcggccg acaatgagcc ggcagcaatc aaatcaggat aattgacgac ttcgccagca
    10021 ataaagcctc gctcaaaatc gccatgaatc acgccggcgg cctgtggtgc cgtggcgcct
    10081 tgcggaatag tccaagctcg cacttctttc tcgccagccg tcagataact ttgcaatccc
    10141 agagtatcat aggcagcatg gatgagttgt agcagccctg attcttgctg accgtagctc
    10201 gctagaagtt cagcggcatc ccgagcttcc agatctttca actcgctctc cagcttagcg
    10261 caaacaaaca gggcccgggc aggagccacc atttcacaca attgctgacg gcgagcttca
    10321 tcgtccaaac ccttctcgtc gagattgaac acatagatga tcggtttcat ggtcagcaac
    10381 tgcaaatcag caaccgcctc ggcgtcgatt tccggatgat tccaaagcgg ttcaccgtca
    10441 ttcaaaaaag tattcacttg ctgataggta tcgacgagcg gtttcaattt cggattagcc
    10501 cgcgcttctt tctcgagctt cggcagccgg cggctgagag tctgcagatc ggccagcacg
    10561 agctccgtat tgataacatc gatgtctttt tttgggtcat gcgcttcatc gacatgtgtg
    10621 acattcgggt cttcaaacgc ccgcacaatc tgtgcaatag cattggtact tcgtatgtgg
    10681 ctcaaaaact gattgcccag gccttcaccc tcactggcgc ccgccaccag tcccgcgatg
    10741 tccacgaacg tcaccgtcgc cggcacaatt ttgtcggtat gatacaactc cgccagtttc
    10801 tgtaagcggg gatctagtac cggcaccacc cccgtattcg gctcaatcgt cgcaaacgga
    10861 taattcgccg ccaaaatatt actccctgtc agcgcattaa acagtgtcga tttcccaaca
    10921 ttcggcagcc cgactatgcc tattgaaaga gatgacatag ttactagtgt aacagataga
    10981 agcaccttga caaattaata aaaatttgct aatatacttg tagctccaaa aacaaaaata
    11041 tgcccgaaat agttaagcaa ccacatacct caactgaaca agtcaagcag tggcttaatc
    11101 atgacttact ccttttagct ggtggagcag aaatcgtcaa tggccaactt gacccaactg
    11161 tcgtacaaaa ggaacacatg gccgcggaga tgaaagcttc gcttgaagcc aagcagcagc
    11221 gccatatcga aatacttggc gatttagccg taaagcatgc tggcccggac actgaagaag
    11281 ctttgctaca aagagatgta gctgaaaaat cccaaatgaa agaaagaata gatcattggc
    11341 tagacacagg agtaattaaa ttaaccacgt ggctaggaag aaatcgaatt gcacgcgaat
    11401 ataatccaag acagtacagt aatgctttca cggacttaca aaataagccg ggggctgccc
    11461 tcatcaaggc gcgccgcatg ccatttgcca aagaattact agccatgggc gtttcagaag
    11521 tcgttacagt ttccaaccct cagcagaagt ttggcagatt tgatagaaag tcacctcaag
    11581 cagatgagga tgccgtacaa atagattaca aaacattaac gggtgaaacc gctaaagggt
    11641 cgccttacaa atatcgcagc aaagttgttt cagggaactc tctttcagtc ggcatagtat
    11701 tacctaagac cgaagctgat caatttgtgc aagctgtcca cgacgatcct caggttatgc
    11761 gagaactggt agaagctctg atggctgaaa gatttgacgc gggggaaagc tgggaaaatg
    11821 ctcgcccctc atacgaggac tggcgcaatg tgaacgaagg cgtaaatcgt atagcctttc
    11881 gcccaacccc tactacctat ccagctcaaa gcgaagccct gacattttaa cagtaactta
    11941 ccggcatcga agctactgac ggtagtctgc tatgcttata cataagcgat atgcagcagg
    12001 atcaagttcg gttaaatgcg gccattgaag aaggcgtgtc caaaggcgtg cctgtggatt
    12061 tcattgctta tagtttgacc agggcgggct ggccgcagga tatggtcaag gaagcgctcg
    12121 atacctggct gctggagaac ggccgcaggc aaaaaacgac cggattcacc gagtggctgc
    12181 gcaaatacta tgctcaggcc aagccggcga tcatcttgat ggtggtgcta aatacgattg
    12241 cttcagccat cgcgctattg cagccttggc cgcttaaaat cttggccgat tcggtctttg
    12301 gcagtgtgcc ggcgccgggt gtcctccggc catacacgca cacgccgaaa cttattctca
    12361 tcgtctcgct tttgacactg ctgattttcc tattggggca agggtttggc gtgcttaaag
    12421 atttcctgct gctgaaaatt gattattggc tgaaccgctc gatcaaagag gaggctttcc
    12481 ggcacatact gcatttgccc ctgtatcatg aggggcgcct gcccaaaggc gactatattt
    12541 atcgccaaaa cgaggtaacc aatagcttgt ctgatctggt tcttaatacc acgtcggaga
    12601 tgattggctc gttcatactg atcatcggcg tgacggctat catgctgacg ctcaatgcca
    12661 agctgacgct tattacactg tttgttatcc cgttcctgat attctccgtc ggtgttttcg
    12721 ggccaatcat ggggcggctg gggcaggctt tgaccaagct cttcagccag acctcggctc
    12781 tgattgccga atcaatcgac aatagcgaag cggtgcaggc ctttacgctg gaggagcgcc
    12841 aggtccagaa gctgcgctca ctctggatgc agacatatgt tgtgtcgcgc cgtgggctgc
    12901 tctggggcaa ggccttcaat tttagcaacg gattggtggt gattctcggc acctcactgg
    12961 ttatatattt tggcggcact gaggccttgc gcggccaatt ctccttgggt tcgctgctga
    13021 tttttatgac atacctcggc tacttgatta atccgatcca ggacatcacg gctcaaatta
    13081 ccattcgccg gcagaaactg gtcaatgtcc ggcgagtcta cgaagtcttg agcgatcacg
    13141 agggcattga acgcctcagg gccgaccggc cgttgccgcg tgtcacagga ggagtgaaat
    13201 tccagaacgt tacctatgtc agtggcgggg tgaaggtcct tgatcaggtg aacttggaca
    13261 tcgcgccggg gcagaagatc ggcattattg gtcccagcgg ctccggcaaa accaccttgt
    13321 tgcgcctgct tgatttgtac atcgaaccga cagcggggcg tattttgctc gatggtcacg
    13381 acatccagtc cgtcagcctg cacgacctta ggcgcaatat cgcttggatc agccagaccc
    13441 cgcagctatt tgctacctct atcgctgaaa atatgcaaga tggcgacatc gagcgggtga
    13501 tgacgcccga ggaatttagt tgggcagcgc aggcagccaa tattaccgaa tttgttaacc
    13561 gattgccgat ggggatgaat tcggtggcgg gcgagggcgg atcttcctta agcggcggcc
    13621 agaaacagcg catttccatc gcccgggcgc tgctcaaaaa cgccccgatt atctgcatgg
    13681 acgagccgac ttcagccctg gacgacaagt ccgaaaaact gattcacgat tcgatcggtt
    13741 cgcttatcca gggcaaaacc gtgctgctgg cgacgcaccg tttggcgctc ctaggcctga
    13801 tggacaaggt gtacgtggtg cagggaggcc acctagtgga cgtcaacgag ctaggcgggg
    13861 tcgagcgcta cacatatcag ctgcaggtcg gcggccaact ctagttgaca tctgtatagt
    13921 ggttatggta aaatcaggcc ataattttat aaaacaaaca taaggcaaaa aaatgacacc
    13981 cgaagaagaa aaattattgg ccgagtctat tacgctccac accgacgcta gaaatgctgg
    14041 cgctagaaaa atgtatgatg acagaaccgg taataaagtc gcggctgcta atgatgcacg
    14101 catacagggc atagacaatg cgttggctgc cccgatagac gcacaactaa aggcgctaga
    14161 aagcgccccg gttccaattg cagctactcc tgccgcaccc gccgcaccca agcctgtccc
    14221 ggtcgccgat gcctggggtt cgtcaacacc cacagcttgg ggtcagccaa tagcgaaacc
    14281 atcaactccc aaaccggtaa ctcaccccag cggcggagcg ccggttgatc ccgcgcctcc
    14341 tgcacacaag cctgccccgg tcgctgatgg ttggggttcg tcaaaaccaa cagcttgggg
    14401 tgagccaatt aataaaccag ctactcccaa acccgacact catcctacgg gcgcgggtag
    14461 ccctaccatt cacgatggct ggggttcgtc aacgcccaca gcttggggcg aaccaacagc
    14521 aaagccatcc actcccgcgg ctgacaaacc tccggtagct acaggtttcg gaactggtgg
    14581 tgatgtgcac cccattcata atcctctctc aggaggctca gccaccggtc atactaatgc
    14641 cccaactgcc agccccccgg tcccagctgg gcactcgaca tctgaagggg cttctacgca
    14701 cactgctgct cccgcgtcgc attctgatgg tggcgcacct gtagagggca ctgcgtctta
    14761 cagcgtggta aaaggcgaca ccctgagcgg tattgccgcg gcccatcata tgtcattgtc
    14821 cgaggtggag tcgctcaacc cgcagattaa gaaccctaac ttgattcttc ctggtgagac
    14881 cgtgcacctg agtgccagca tgcccaccag ctcaagttat tccggcatca gctccagtgg
    14941 tagcggcccg gcacaaatgg tgcccggcgt caccgacacc tccacctcca acaaacgggg
    15001 tattttaggt agtctgctcg gccgattcgg caaaggtggc aacaccgacg gcggcgttcc
    15061 tccagcgacc gaccccgcca aaacagtagc ggttgatccc accaaatccc aaaaaaccga
    15121 gccgactaag ccacttagtt aagccgttct acgctacgtc gttcgcgagc agttgctcga
    15181 ggctttcccg tagtctcggg aagccctcga agggcagggc ggtcatgccg agacttgcgg
    15241 caaattgcct gtaagccggg ttgccactga ccaccaaaca atttcgtggc ggctcaccga
    15301 tttgttgcaa ggccatagcg aaaacacttt gactgagcag cggttcaccg atggtaaatg
    15361 gcagcggtcc ggcaataacc tcgaacaatt gataacgatt attaagcccg ctcagcagcg
    15421 cgtactcttg cgtgccgaga ttggccaaga cgccaagctt caggaagtgg ccgtgtaagc
    15481 ttcgcataaa atcgatgagt gccggtgaga tactattctc gttcagcgta aactcggcag
    15541 catctatttc gggccggctg agcttgaacc taaaagtgtt ggccacttct tctggcgtcg
    15601 acatgccatg gaagtacttg ctgacaagta cgttgagcgg gccggcgacc tcagtgccgt
    15661 attgttcagc cagctgaagg tattgagcaa aaacatcggg tgtccaaacc ccgtagaaat
    15721 caaagaaaac agcgcgaatc acaccttaca ggatagcaaa aaagccaggt gatgactact
    15781 atactaggga cgcttggtct gagatctttg gctctgaagc ggacgactaa gcgctgacgg
    15841 ccaccagtta gcgcggccca gcagggcgac gatcgagggc actagcagtg tgcgcacaaa
    15901 gaatgtgtcg agtaagatac caaaagcaat gccgaagcca atttgctgga cttgctcgtt
    15961 gccaccgact ataccgagga tagtaaaggt gccggccaag atgagcccgg cggaagtgac
    16021 agtggtgccc gtgatgccga cggctttcgt gactgcctct gacagggatt tgtggtcgtg
    16081 ggcctcttcg cggatacggc tcatgaccag aatgttgtag tcttcaccga gagccatgcc
    16141 gaagatgaac atcaggaagg gcagcacgaa gttcagtccg tcttgatggg ctatatgtac
    16201 gaacacgatc atagcgaagc cgaggctcgc gagatatgag aggcctacgg tgagaatcag
    16261 gtaccacggc gcgaccaggc tgcggagcag caatgcgagg agggcggcga taatcagtag
    16321 cacaactgga atgatctttt ctaggtcgct agtggcaatg cggttaacgt cgtagccaac
    16381 cgagtcggag ctgtagattt ggctttggtc ggcgcccacg cccgcggcta tggtttgtag
    16441 ttcgtgccgg acggttggaa tagcggcagt ggctgccgaa ctgccggtca ccccggcttt
    16501 taacacgccg tagaactgga ccgttttacc atccgggctg atgaactgat tgatggcttg
    16561 cagggcgggg ctggtgccgc tttggtgcag ctgggcgagc tgctgaggtg tcaaacgaaa
    16621 gccattggca ttaaacggac cggcgacagc ggtgaaggtc ttcgaggcgg ccaacttatt
    16681 ttctacggtt gcgactggcg acagattatt ccataccgaa gcggggaaat ggaaaatgag
    16741 cagctgcggg ttattgttgg cggccgggaa atgctgttgc aggatttgct ggccggctgc
    16801 cgagtcgctg ccgctcggcg cgctgccttg atcgccaaaa ccagccgatt tgtagccgac
    16861 tatgccgagg ctcaggccac cgaagacgag ggcgcccaga accaacatca tgatcggctt
    16921 tttgatgaca ttgtcagcca gacggcccca caggccgatc ttaagtttcc gttttgacgt
    16981 cttggagggc cagaaaaccg cgcggcccaa gatggccagc aaggcgggca ggaacgtcaa
    17041 agcgattagc agcatgacag ccaggccgat ggccagagcc gggcccaggc ctttgtagag
    17101 gccaaacgag gccagcagca agctgaggag ggcagcggcc acggtcgctg ccgaaaacgt
    17161 aatcgattcg cccactttgg acaaggcttt gacgactgct tcctttggct ccaggccgcc
    17221 tcgcagctct tcacggaccc ggaaaaccag gaaaagaccg tagtcggtgc cagctcccag
    17281 cagcaacacg atgagtaaga tttgcgtgat gaagccgact tggacgccga tcttggtcga
    17341 ttcggcgatc accggctgag cgacggccag tgcgataccg gccgggacca aggtcacgat
    17401 aggtgctaac agggcccgga acacaacgag caacagcact agaatcaaga tgacggtgta
    17461 gccttcggtg ctgttgcggc ctttttgatt ggcgttttcc tggtcgaccc cggctgctag
    17521 gtcgccggtc aagtgtgcgc tgagcccgga ggggaccccg ctttgcttaa tcgcgctgcg
    17581 aatattttta acgatgtcgg tggctttttg gccaaaggcg tcgctggaaa taccgacgag
    17641 gtattggtgc acctggccgt ccgcgctggt gccggaatcg cggacttcag taaccgacgg
    17701 gacgtgctta acggcggtga ctactcgctg cagggcgggg gtatcgctac tggttagtgg
    17761 cccgctagta ctgctgacca cgatgatggc gttggtggcg gtctttttgt tctgaaaggc
    17821 ggtctcgagc ttggaggcgg tgtcggtcgg gctatttttg ggcaaaaagt ccgattcgtt
    17881 atttttggtg acattattga tattcggaaa gttagcagtg acgactggaa tagctacaat
    17941 ccagaaaacg ataatgaacc aacgaaagcg aactgaaaac cgcgcgattt tttcgaacat
    18001 tagagcccct taacaagtta cctatttact atatcagagc acgcctgtgt cgtaataatt
    18061 gacatagtca attattgaca tgtacaatta tcgcatggca aatggcgcag ctgtacttgg
    18121 tgaagaagcc gtgaaagcga tggagacgaa aaagac
//
LOCUS       DATNGP010000127         3374 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_723727, whole genome shotgun sequence.
ACCESSION   DATNGP010000127 DATNGP010000000
VERSION     DATNGP010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3374
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_723727"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1054)
                     /locus_tag="VN778_03265"
     CDS             complement(<1..1054)
                     /locus_tag="VN778_03265"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF020892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HXR50022.1"
                     /translation="MSNYYVTTSIPYVNGDPHIGFAMELITGDVLARAARAAGHTVIY
                     STGTDEHGTKVAEKAASLGKTPQEFADEVSAKFRTLGELLDVEPDRFIRTTDKAHMDR
                     AQLIWKALSQDIYKAKYVGWYDVRQEEFVPESQADPERMKPEHPQAYQKLEEENYFFR
                     LSKYNERILQAIEADELQIVPKTRKNEIISLLKEGLDDISISRPAEKLEWGIPVPGDK
                     KQVMYVWFEALMNYVTVLGYPEHDDFKKFWPAQVQIVGKDIIRFHAAIWPAMLMGLGI
                     GLPKQLYVHGFVNVDGQKMSKSLGNVIAPSEIVEKYSADAFRYYFLRHIPSYDDGDFS
                     WEHFEAAYNNELANELG"
     gene            complement(1231..1647)
                     /locus_tag="VN778_03270"
     CDS             complement(1231..1647)
                     /locus_tag="VN778_03270"
                     /inference="COORDINATES: protein motif:HMM:NF013401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HXR50023.1"
                     /translation="MSTALCPFCASKDRVLKENESAILILSDPRKVPGHILVLPKRHV
                     EEPWELMSNELRDIFELIFFAERRLLGKLGEGVDIRQNYRPFKKQDKLKANHVLFHVI
                     PRAKDDYLYTVSEKFERDLFAELDNLERKEVEKLLD"
     gene            complement(1751..2242)
                     /locus_tag="VN778_03275"
     CDS             complement(1751..2242)
                     /locus_tag="VN778_03275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50024.1"
                     /translation="MYNLLVSHNSPLEECMWYQQVLVDETTALPATVLGAHALWEEVF
                     RITSVHEDDREGNPRLARQLVEEYGPFAERRQNGIEWLVEYYLEHFGDEGAALIREHW
                     GARTLSWYLPPEGNPLYVARNKLRRLHGGLIVDDYRLYELGGRLPSGRLRPFAFFVPA
                     PAV"
     gene            complement(2337..2423)
                     /locus_tag="VN778_03280"
     tRNA            complement(2337..2423)
                     /locus_tag="VN778_03280"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2387..2389),aa:Leu,seq:taa)
     gene            complement(2598..2671)
                     /locus_tag="VN778_03285"
     tRNA            complement(2598..2671)
                     /locus_tag="VN778_03285"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2637..2639),aa:Cys,seq:gca)
     gene            3192..>3374
                     /locus_tag="VN778_03290"
     CDS             3192..>3374
                     /locus_tag="VN778_03290"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXR50025.1"
                     /translation="MKLLVIDDNLALVRSLKDFLGKDFVIDTERTAKEGLRQAITNSS
                     DVIVLDLGLPDGSGSDV"
BASE COUNT          862 a          882 c          759 g          871 t
ORIGIN      
        1 tgcccagttc gtttgccagt tcgttgttat aggcagcttc aaaatgctcc cagctaaagt
       61 cgccgtcgtc atagctagga atatgccgca ggaaataata gcggaaggca tcagcgctat
      121 acttctcgac aatctcgctt ggcgcaataa cattgcccag gctcttactc attttttgac
      181 catcaacatt cacaaagcca tgcacatata gctgcttcgg caagcctatg cccagcccca
      241 ttaacattgc tggccagatg gccgcatgaa aacgaataat gtccttgcct acaatttgca
      301 cctgcgccgg ccagaatttc ttaaaatcgt catgctcagg gtagcccagc actgtaacgt
      361 agttcatgag cgcctcaaac catacataca tcacctgctt tttatcgcca ggcaccggaa
      421 taccccactc cagcttctcg gctggacgtg aaatgctgat gtcgtccaag ccctctttga
      481 gcaggctaat aatctcgttc tttcgggtct ttggcacaat ttgcagctca tcggcttcga
      541 tagcttgcag aatccgctca ttgtacttac tcagtcggaa gaaataattt tcctcttcca
      601 gtttttgata ggcctgtgga tgctctggct tcatacgctc tggatcggcc tgtgattctg
      661 gtacgaactc ttcctggcgc acatcgtacc agccgacgta cttcgcttta taaatatctt
      721 gtgacaacgc cttccaaata agctgcgcgc ggtccatatg tgctttgtcg gtagtacgaa
      781 taaagcgatc gggttccaca tcaagcagct caccaagtgt tctaaactta gccgatactt
      841 catcggcaaa ctcttgcggt gtcttgccta ggctcgctgc cttctcggca accttagtac
      901 catgttcatc tgtaccagta gagtaaatga ccgtgtggcc ggcagcacga gcagcacgag
      961 ccagcacatc accagtaatc agctccatgg caaagccgat atgcggatcg ccgtttacgt
     1021 atggtatgga ggtggttacg taatagttac tcattgtttt actgtcctct cggtattctt
     1081 agccttatgc ggtattacac caactttcaa agaccattgt acccgtttag tttatgcaag
     1141 aaaaaataga taaagaaaag ctatgcccgc gggggcatca tcataacaac tgacatgcgg
     1201 aagcttttca tagcaagggc gattatatca ttagtccaga agtttttcga cttctttgcg
     1261 ttctaggtta tcaagctcgg caaacagatc acgctcaaat ttttcagaaa ccgtgtacaa
     1321 atagtcatct ttagcgcgag gtatgacatg aaacaggaca tgattggcct tcagcttgtc
     1381 ctgtttttta aacggccgat agttctgacg gatatcaacc ccctcaccca gcttgccaag
     1441 caagcggcgt tcagcaaaaa agatcagctc gaaaatatcg cgtagctcgt tgctcatcag
     1501 ctcccacggc tcttccacat ggcgcttggg caagacgaga atatgaccgg gcaccttacg
     1561 gggatcactt aaaattaaga tagctgactc attttccttg agtacacggt cttttgaagc
     1621 acaaaaggga cataacgcag tagacatacc ctatagtata ccctgttgat gcccaccccc
     1681 atagatgtat aaatcgatga ttcgggcgcc gcactccgca gggagtagta gagtgggcgc
     1741 ccgaatcatg tcagacagca ggagcaggaa cgaagaacgc gaaggggcgc agacgacccg
     1801 agggtagccg accaccgagc tcgtagagcc ggtaatcgtc aacgatcaag ccgccatgca
     1861 gccgccgcag cttgttgcgt gccacgtaga gcgggttgcc ctccggcggc agataccagg
     1921 agagcgtacg cgcaccccag tgctctctga tcagtgccgc gccttcgtca ccgaagtgct
     1981 cgaggtagta ctccacgagc cactcgatgc cgttctggcg acgctccgca aagggcccgt
     2041 actcctcgac cagctgtcgc gcaaggcgcg ggttgccctc gcggtcgtcc tcgtggaccg
     2101 aggtgatcct gaacacctcc tcccacaaag cgtgagcccc gagaacagtg gccggcaagg
     2161 ccgtagtctc gtcaaccaga acttgctgat accacatgca ttcctccaga ggggaattgt
     2221 gactgactaa taaattatac aattaatagt attatctagc aaatcagtgc atcgagccct
     2281 gcagaaatta atcgaaccat ataaaaacac ttcccctgaa aggaagtgtt tttatatggt
     2341 gggcgaggtg ggacttgaac ccacaagact tttcggtcag cggattttaa gtccgccgcg
     2401 tataccaatt ccgccactcg cccaaggagt tgatttggag gcacctaccg gattatcggc
     2461 ttagcctctt ccctgcggtg ctcgcttaca aattgaaaca cgtttcaatt gtcagctgcg
     2521 ctcctcgttc acgaaccggt tttgccaggt tcaaatgcag gctccaaatt tactcctgcc
     2581 ttcggcagaa ctaaatttgg aggcacctac cggatttgaa ccggtgtacg cggttttgca
     2641 gaccgctgcg taaccactcc gccaaggcgc cacatattgg acccgcttat tgtatcaaga
     2701 aactacacaa atcgtagccc tattttgact taatacccct tgtctcacca cagaccagcc
     2761 taggaatata cttaaatatt tatgctacgt attggtaaaa cagtcagatt tcagtatttt
     2821 ttaatgtttt ttgactatcc ttcttgacaa ggggacataa cattgtatgc gagaggaagc
     2881 ggatatgctg tctggctaag ctgtatgcca ggcgcatgct atgcgatcac tttactgcct
     2941 atactgcctg aggtcgctca aaaaatccac taagcacgtc cactctttac gcttaagtcc
     3001 ctcgcctaca ggttctacat gccattatgg ccccttccta agccaaacga ccagtcgtac
     3061 attcgtatgg ctggttattg gtttatacat gaaatacgta tgtaattctt aaatactcct
     3121 caaatttaat gactatttaa taattaagtt gctagtatga taggtgttaa gcattccagt
     3181 agcgcctgtt tatgaaacta ttagtcatcg acgataacct cgctttagtc cgaagcctca
     3241 aagatttcct aggtaaagat tttgtaatcg acaccgaacg aactgcaaaa gagggcttac
     3301 gtcaggcgat cactaatagt agcgatgtta tcgtcttaga tttgggtcta cccgacggat
     3361 cgggtagtga tgtg
//
LOCUS       DATNGP010000128         3411 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_4073464, whole genome shotgun sequence.
ACCESSION   DATNGP010000128 DATNGP010000000
VERSION     DATNGP010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3411
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4073464"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1021
                     /locus_tag="VN778_03295"
     CDS             <1..1021
                     /locus_tag="VN778_03295"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HXR50026.1"
                     /translation="AAKPHVSKNSTGYNLWDVWDRETGVFDLTKVIVGAQGTLGFVTD
                     IHFKLVPARPHSGLLILFMKDITDLGEVIPKVLEAKPATFESFDDATLWLGVKFMPSF
                     HKMLGWGGVLKLFISFIPTGLKFWRGIPKLILMVEFNGETEDEVRQKVHSLHRELAHH
                     RARYEINGFEEDPTEGKSEKFWLMRRYSFQLLRSKVKDKHTAPFIDDFVVPPPHLTAF
                     LPRLRSIIKKYKLFATIAGHMGDGNFHVIPLMKLEDPKDRKKILPAMKAVNNLVLKYG
                     GSLSGEHNDGLVRGPWLEMMYGKDMLKLFKEAKHIMDPQNIFNPHNKADAKWEYSFGH
                     IRDHF"
     gene            1082..1351
                     /locus_tag="VN778_03300"
     CDS             1082..1351
                     /locus_tag="VN778_03300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50027.1"
                     /translation="MSVQTISNNDLANSLDELKALMLTGFEAMNARFDRLEERVDRLE
                     ERMDHLESRMDSLEAVQRETNVRLDSLEAGQRETNIRLTRIEGSV"
     gene            1385..1522
                     /locus_tag="VN778_03305"
     CDS             1385..1522
                     /locus_tag="VN778_03305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50028.1"
                     /translation="MIANLKKRFGKQLDERTDLKTRVRRVEAFAREVSAQTGIPYPKL
                     D"
     gene            complement(1531..2409)
                     /gene="cyoE"
                     /locus_tag="VN778_03310"
     CDS             complement(1531..2409)
                     /gene="cyoE"
                     /locus_tag="VN778_03310"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: protein motif:HMM:NF003348.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HXR50029.1"
                     /translation="MLAISRLKLYYSLTKPGVLYGNVLTTVAGFLLAAGYKQRFDAGL
                     FIATIIGTTLVIASACTINNYFDRDIDSIMARTKARAVASGKIKGRHAVIFSIVLGAL
                     GLSVLILGVNWLVVAIGLIGFIDYVWLYAMFSKRRSVHGTLVGSISGAMPITAGYCAV
                     TNRVDGGAILVFLALFFWQMPEFYSIAIYRRKEYQAAGVPVISVVKGVKRTKIEILLY
                     TIAFVAATLLLTIFGFTGYLYFVIMALLGSYWLLLAIKGLHRTDSDQWARQMFHFSLV
                     VLLAYCLALSIGALLP"
     gene            complement(2403..2783)
                     /gene="cyoD"
                     /locus_tag="VN778_03315"
     CDS             complement(2403..2783)
                     /gene="cyoD"
                     /locus_tag="VN778_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003021682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009486 - cytochrome bo3 ubiquinol oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HXR50030.1"
                     /translation="MSARPVISHHEESHGSFKSYTAGFVLSITLTLLAYAFASHQVAR
                     GWGLIYLLAVLAVIQLFIQLVFFLHLGRESKPRWNLTVFSFALMVVVIVVFGTLWIMK
                     NLSYDHHTLSPQQASQYLVKDEGY"
     gene            complement(2780..>3411)
                     /gene="cyoC"
                     /locus_tag="VN778_03320"
     CDS             complement(2780..>3411)
                     /gene="cyoC"
                     /locus_tag="VN778_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011062990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009486 - cytochrome bo3 ubiquinol oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HXR50031.1"
                     /translation="RSGASQMSTALTAKELHRSNQEREATSKTLFGFWVYIMTDCVLF
                     ASLFATFTVLRNSTNGGPSGADLFSLPFVLAETVALLTSSYTAGLGLLAARRRDKNQV
                     FFWFGITMLLGIFFLTMEISEFHKLVLEGNSWRRSGFLSAFFTLVGTHGLHITTGLVW
                     MTVMLYKIYNKGVTAVNLKRLTMLSLFWHFLDIIWIFIFTIVYLIGVKA"
BASE COUNT          971 a          789 c          859 g          792 t
ORIGIN      
        1 agctgccaag ccacatgtca gtaaaaactc cacgggttac aacttatggg atgtctggga
       61 ccgcgaaacc ggtgtgtttg acctgactaa ggtaattgtc ggagctcaag gtaccctcgg
      121 atttgttact gatatacact ttaaattggt gcctgctcgc ccgcattccg gattgcttat
      181 tttgtttatg aaagacatta ctgacctagg cgaggtgata ccgaaggtgt tagaagctaa
      241 gccagcgacg tttgaatctt ttgatgatgc caccctctgg ttgggcgtta aattcatgcc
      301 gagttttcac aaaatgctag gctggggcgg tgtactgaag ctgttcatta gttttatccc
      361 gactggtctc aaattttggc gagggattcc gaagctcatt ttaatggtgg aatttaatgg
      421 tgaaaccgag gacgaggtac gccaaaaagt tcacagcctc caccgcgaac tagctcatca
      481 ccgggcccgg tatgaaatta atggcttcga agaggacccg acagaaggca aatccgaaaa
      541 gttctggcta atgcgccgct atagtttcca actcctcaga agcaaggtca aagacaagca
      601 caccgcacct tttattgatg atttcgtggt gccgccgccg cacctgacgg ctttcttgcc
      661 ccgcctgcgg tccattatta agaagtataa actgtttgct actattgccg gtcacatggg
      721 tgacgggaac tttcatgtaa tacccctgat gaagctggaa gatcccaaag accgcaaaaa
      781 gattttgccg gccatgaaag cagtcaataa tctggtgctc aagtatggcg gttcattgtc
      841 tggcgagcac aacgacggct tggtacgcgg tccctggctg gaaatgatgt acggcaaaga
      901 tatgctcaaa ctctttaaag aggcaaagca tattatggat ccacaaaaca tctttaaccc
      961 gcacaacaag gcggacgcca agtgggaata ttcatttggc cacatccgtg atcatttcta
     1021 gtctgaaatt tttcttaaac tattttgggc gatgtcaaac gcggcgtata cttgtttcac
     1081 catgagtgtg caaacgatta gtaataacga cttagccaat tctcttgatg agttgaaggc
     1141 cttgatgctc accggttttg aggcaatgaa cgctcgtttt gaccgtttgg aagagcgtgt
     1201 cgaccggctg gaagaacgca tggaccactt agaaagtcga atggatagct tagaggctgt
     1261 gcaacgggaa accaatgtca ggttggattc cttagaagct ggccagcggg aaaccaacat
     1321 tagattgacg aggatagaag gcagcgttta gtcgcactat gccgacatta aagagctata
     1381 ccgtatgata gccaatctca aaaagcgctt tggtaaacaa ttggatgagc gaacagattt
     1441 gaaaacacgt gttcgtagag ttgaagcttt tgcacgcgag gtttcggccc aaaccggcat
     1501 tccctatcct aaacttgatt aggctggcgg ctatggcagg agggcgccta tggagagtgc
     1561 taaacagtat gcgagtagaa caacaaggga gaaatgaaac atttggcgag cccactggtc
     1621 gctgtctgtg cgatgcaaac cctttatggc tagtagtaac cagtagctgc caagtagcgc
     1681 cattatcaca aagtacaggt agccggtgaa gccaaaaatg gtgagcaaaa gcgtagcggc
     1741 cacaaaagct atcgtataga gcagtatctc tattttggtg cgcttcacgc ctttcaccac
     1801 agagatgact ggcacgccag cagcttgata ctctttacgg cggtagatag ctatagagta
     1861 aaattcgggc atttgccaga aaaagagggc caggaaaacc aatatggcac cgccgtcaac
     1921 acggtttgtg acggcgcagt agccggcggt gatgggcatg gctcccgaaa tgctgcccac
     1981 cagggtgccg tgcaccgatc ggcgcttaga gaacatcgca tacagccaga cataatcaat
     2041 aaacccgatc aagccaatag cgactacgag ccagttaaca cctaatatga gcacactcag
     2101 accgagagcg ccaagaacga ttgagaaaat gacagcatgg cggcctttaa ttttgccgct
     2161 agctacagct cgtgctttgg tccgcgccat gatgctgtcg atgtcacggt cgaaataatt
     2221 gttgatggtg caagctgatg caataaccag cgtcgtgcca attattgttg caataaatag
     2281 tcccgcatca aatcgttgct tatagccagc cgccaacagg aagccggcta ccgtagttaa
     2341 aacattccca tacaagacgc caggtttagt cagcgaatag taaagcttta gacgactgat
     2401 tgctagtatc cttcatcctt aactaaatat tggctggcct gttgtggcga caaagtgtga
     2461 tgatcataac ttaagttttt cataatccag agggtgccga aaactacaat gacaacgacc
     2521 ataagagcga acgagaatac tgttaaattc cagcgcggct tggattcacg accaagatgc
     2581 aagaaaaaga cgagttgaat gaagagctgg atgaccgcca acacagccaa taaataaatc
     2641 agcccccagc ccctggccac ttggtgagag gcaaaggcgt acgctaggag cgtcaaagta
     2701 atcgatagca caaaacccgc agtatagctt ttaaagctgc cgtggctctc ttcgtggtgg
     2761 gagatgacag gccgagcgct catgctttta cccctataag ataaacaatc gtgaagataa
     2821 agatccaaat gatgtccaag aagtgccaaa agagactcag catagtgagg cgtttgaggt
     2881 taactgccgt cacaccttta ttgtatattt tgtaaagcat gacagtcatc cagaccaaac
     2941 cggtagtgat atgcagaccg tgcgtgccga ctaaagtaaa gaaggctgaa aggaagccgc
     3001 tgcggcgcca gctgttacct tccaaaacca gtttatgaaa ttcacttatt tccatagtca
     3061 ggaagaaaat gccgagcagc atcgtgatgc cgaaccagaa aaagacttga tttttgtccc
     3121 ggcggcgggc cgccagcaaa ccaagaccag cagtatagct actggtgagc aaggccacgg
     3181 tctcagcgag tacaaatggc aggctgaaga gatctgcgcc ggacgggccg ccattggtgc
     3241 tgttcctcag cacagtgaag gtagcaaaca aactggcaaa cagcacgcaa tccgtcatga
     3301 tatagaccca gaaaccgaac agcgtctttg aggtggcctc gcgctcttgg ttagatctat
     3361 ggagctcttt ggcggttaga gctgtactca tttgagacgc tccgcttcta g
//
LOCUS       DATNGP010000129         3997 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_3801903, whole genome shotgun sequence.
ACCESSION   DATNGP010000129 DATNGP010000000
VERSION     DATNGP010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3997
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3801903"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1550
                     /locus_tag="VN778_03325"
     CDS             <1..1550
                     /locus_tag="VN778_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008578992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HXR50032.1"
                     /translation="RQYHAVVFDVDGETKLIAMEDPDDIQALNFLQKQLGNNIKVHIT
                     TSSLLQAALDQYRGNISSELTKVIADEDEVDESDEVDESDLAEDSPVAQTVNIIIEYG
                     VKSGASDIHIEPREDYVVVRYRIDGLLREANKLPRKLLGALVSRIKILSNLKIDEHRA
                     PQDGRFKVEVSGQVYALRVSTLPILDGEKIVMRILNESSKPSDFAELGFWGMALTDLQ
                     HAIVQPHGMVLVTGPTGSGKSTTLFSVLSSLNTPNVNISTVEDPIEYHIIGANQTQVN
                     PIAGMTFVNGLRALLRQDPNIIMVGEVRDGETADLAVQAALTGHLVFSTLHTSNAATC
                     LPRLLEMGVEPFLIASTVRLIIAQRLVRRLCIDCRESYSPDASVIKQLTKSFQLDDNG
                     GLKQLHELETQALEGGIGKSTNDKGASSNELSTTAERITRLWKAHDDGCDHCNHTGYR
                     GRIGIYEALNNTAAIQKMIVTHSTSEDIEQAAIDDHMLTMQLDGLVKALRGQTTIEEV
                     LRVTAQE"
     gene            1555..2829
                     /locus_tag="VN778_03330"
     CDS             1555..2829
                     /locus_tag="VN778_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HXR50033.1"
                     /translation="MLFSYKGTNKEGRSVSGTAEASDKIALVGLLTKQGIHPVLIKPA
                     KHKAGQKGSLFGPKKKVKLNDLVIFTRQLSTMVSAGVPLARSLSALQTDAENPYFREV
                     LAGITKEVEGGQPLGDAFAKYPTVFSDVYVNMVRAGEEGGILDQILKRLATQVEQDAS
                     MRKKIKSAMMYPMVIMGITVVAFFGVMLGIVPKLGAILTSLGGPNAKLPIYTQVLLNI
                     SNFCAKPTLLKHIPIPVIDHLPNLILIAGALVIGIIYLLRYIRTPKGQYKFHALLLRI
                     PIVKIVILKIAIARFSRTFASLMGAGVSVLDALDVTGAALGNRVIEAELKAAAQEVKN
                     GKPLSAPLSQSPHFPPIVSQMLLVGEETGQIDTVLVKIADFYEEEVAVLIDGLSAIIE
                     PIIIVVLGAGVGLIAASVMGPIANLSKNIGNS"
     gene            2966..3439
                     /locus_tag="VN778_03335"
     CDS             2966..3439
                     /locus_tag="VN778_03335"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HXR50034.1"
                     /translation="MISLKKRNQGFTIVELLIVIVVIGILALLVITTYSGIQAKARNA
                     KRQSDLKSIQTQLEAFFSQNGYYPSLTDLNGSSWRSTNMKSLDQNALIDPSNPTQSTT
                     LTTGTTPSKQYAYNVTDSSGASCETTDTNCAKYTLVAGYEGTVNGQTTDSLSNLD"
     gene            3507..3935
                     /locus_tag="VN778_03340"
     CDS             3507..3935
                     /locus_tag="VN778_03340"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HXR50035.1"
                     /translation="MKTFRRRWTRHRQSSTSSKGFTIVELLVCIALAGVVITSLSQVV
                     TTYLHVSQRGRYLNLANSYAEAKVETLRNQGYNSLTLGTTSLTSELSSQLPLTRSASM
                     TVSSPSTGLKQIDLTVTYADQGQSHSYYYTTYIGELGVGQ"
BASE COUNT         1003 a         1097 c          996 g          901 t
ORIGIN      
        1 cccgccagta tcacgccgtg gtttttgacg ttgacggcga aaccaagttg attgccatgg
       61 aagatccgga cgatatccag gcgcttaact tcttgcaaaa gcagctgggc aacaacatta
      121 aagtacacat cacaaccagc agcttattgc aggcagcgct tgatcagtat cgcggcaaca
      181 tcagcagcga gctgactaaa gtcatcgctg atgaagatga ggtcgacgag agcgacgagg
      241 tcgacgagag cgatctggcg gaagattcgc cggtcgccca gacggtcaac atcatcattg
      301 aatacggcgt gaaatccggg gcgagcgata tccatatcga accccgagaa gattatgtag
      361 ttgtgcgcta tcgcatcgac ggcttgctta gagaagccaa caagttgccg cggaagttat
      421 tgggagcatt ggtcagtcgc attaaaattc tcagcaatct gaagattgac gagcaccggg
      481 cgccgcaaga tggccgcttt aaagtcgagg tcagcggtca agtgtacgcc ctccgcgttt
      541 cgaccttgcc gatccttgac ggcgaaaaga tagtgatgcg catacttaac gagtcaagta
      601 agccatcgga tttcgcggag ctcggattct ggggcatggc tctgactgac ctgcagcatg
      661 ccatcgtgca gccgcacggc atggtactag tgacggggcc gactggttcc ggcaaatcta
      721 ctactctgtt tagtgtacta agctcgctca acacgccgaa tgtcaacatt tctaccgtcg
      781 aagatccgat cgaatatcat atcatcggtg ccaaccaaac gcaggtaaat ccgatagccg
      841 gcatgacctt tgtgaatggc ttgcgcgccc tgctccgaca agaccccaac attatcatgg
      901 tcggcgaagt tcgggatggc gaaacggcgg atctggccgt gcaggccgca ctgaccgggc
      961 acctggtgtt tagtacgctt cataccagta atgctgcaac ctgcttgccg cgcttgctag
     1021 aaatgggcgt cgaaccgttt ctgattgcca gtactgttcg gttgatcatc gcgcagcgct
     1081 tggtacgccg gctgtgcatt gattgccgag agtcgtactc accagatgct tcggttatca
     1141 agcagctcac gaagtcgttc cagctcgatg acaatggcgg tcttaaacag ctacacgagt
     1201 tggaaaccca ggctttggag ggtggcatcg gcaagtccac caatgacaaa ggcgccagct
     1261 caaacgaatt aagtacgact gctgagcgca ttacccgact ctggaaagcc cacgacgacg
     1321 gctgtgatca ctgcaaccac actggttatc gaggccggat tggcatctac gaagctttaa
     1381 ataacactgc tgccatacaa aaaatgattg tgacgcatag cacttccgag gacatcgagc
     1441 aggccgccat tgacgatcat atgctaacga tgcagttgga tggcctggtt aaagctttgc
     1501 gtggccaaac cacaatcgag gaggtgctgc gcgtgacggc gcaggagtaa ccccatgctt
     1561 ttctcatata aaggcaccaa caaagaaggc cgctcggtga gcggcacggc tgaggcgtct
     1621 gacaaaatcg ctctggtcgg cctactcact aaacagggca tccacccagt gcttatcaag
     1681 ccggctaaac acaaagctgg gcagaagggc tcactattcg gccctaagaa gaaagttaag
     1741 cttaacgacc tcgtcatctt cacccggcag ctatcgacca tggtcagcgc cggtgtgccg
     1801 ctagctcgct cgctgtcggc cctccagact gatgcggaaa acccgtactt ccgcgaggtg
     1861 ctggccggca ttaccaaaga ggtcgaaggt ggccagccac ttggtgacgc tttcgctaag
     1921 taccctaccg ttttttctga tgtttatgtc aatatggtcc gggccggcga agaaggcggt
     1981 attttagatc aaatcctcaa gcgtctggcc acccaggttg agcaagacgc ctcgatgcgc
     2041 aaaaaaatca aaagcgccat gatgtacccg atggtcatca tgggcattac cgtggtcgcc
     2101 tttttcggtg ttatgctcgg cattgtgccg aagctgggcg ctatcctcac cagtcttggc
     2161 ggtccgaacg ccaaactacc tatctacacg caagtcctcc tgaatatcag caacttttgt
     2221 gcgaaaccaa cgctactcaa acacatacct atacccgtta tcgatcacct gcccaacttg
     2281 atactcattg cgggcgcgct ggtgattggc atcatctacc tgctgcgcta catccgaaca
     2341 ccaaaaggcc agtataaatt ccatgcctta ctgctgcgca tcccgatcgt caaaatcgtc
     2401 atcctcaaga tagccatcgc gcgcttttct cgcaccttcg cctcactgat gggcgccggt
     2461 gtcagtgtat tggacgcact ggacgtgaca ggtgccgccc tgggtaatcg ggtcattgag
     2521 gctgaactca aagctgccgc acaagaggtc aagaacggca agccgctcag cgctcctctc
     2581 agccagagtc ctcacttccc gccgatcgtt tcccaaatgc tcttggtggg cgaagaaacc
     2641 ggccaaatcg acaccgtgct cgtcaaaatc gctgactttt atgaagaaga ggtcgctgtg
     2701 ctcatagatg ggctgtcggc catcatcgag ccgatcatca tcgtcgtgtt gggcgccggc
     2761 gtcggcctga ttgctgccag tgttatgggc ccgatcgcca acctctccaa gaatatcggc
     2821 aacagctaat tagagatttt tccacaacgg gcttgacctc tagccgctta tggttataat
     2881 catagccaga tacttgaaac aagtagtctt cacttgcgtc aaagatcaaa ttaaaaaata
     2941 atagaaatca ggagggtggg ctcttatgat ctcccttaaa aaacgtaatc aaggcttcac
     3001 gatcgtcgaa cttttgatcg tcatcgtagt catcggtatc ttggccctgc tcgttattac
     3061 tacctatagc ggcatccagg ccaaggcccg caacgccaaa cggcaatcag acctcaaatc
     3121 aatacaaacc cagcttgaag ccttcttcag ccagaacggc tactacccaa gcttaaccga
     3181 cttgaacggc agctcatggc gctccaccaa catgaagagc ttggatcaaa acgccttgat
     3241 cgacccttca aatccgacac agagcacaac acttactact ggtactaccc catctaagca
     3301 atatgcttat aacgtgaccg atagtagcgg tgctagttgt gaaacgactg atacgaactg
     3361 cgcgaagtac acactcgttg caggttacga aggtacagtt aacggtcaaa cgactgacag
     3421 tttgagtaac ctagactaag ctagtaagtt gtccactgct aaagccccgg aattgccggg
     3481 gctttactat ataattaaac ataagcatga aaacgtttcg ccgtcgctgg acgcggcatc
     3541 ggcagtcaag tactagttcg aagggcttta ccatagtcga gctgctggtt tgtatagcgc
     3601 tggccggggt cgtcattact tcgctgtctc aggtggtaac tacctattta cacgtttcgc
     3661 agcgcggccg ctaccttaac ctggctaact cttatgctga agccaaggtt gaaaccctac
     3721 gtaaccaggg ctataacagc ctaactcttg gcactaccag cttgaccagc gagctatcct
     3781 cgcaacttcc gctcactcgc agcgcctcga tgaccgttag cagtccctcg actggcctca
     3841 agcaaatcga tctgaccgta acttatgccg atcaaggcca aagccattct tactattaca
     3901 cgacttacat aggagaactc ggtgtcggtc aataatgcgc agtctggttt cacccttgta
     3961 gagctcacgg ttgcggtgac tgttatggcg atcatcg
//
LOCUS       DATNGP010000130         3060 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_2527405, whole genome shotgun sequence.
ACCESSION   DATNGP010000130 DATNGP010000000
VERSION     DATNGP010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3060
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2527405"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..517
                     /gene="efp"
                     /locus_tag="VN778_03345"
     CDS             <1..517
                     /gene="efp"
                     /locus_tag="VN778_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010131241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HXR50036.1"
                     /translation="GVPYRVVEYGQKVMGRGGSIVNVRIKSLLDGKVLEKTYKGNEQI
                     SGADVASQNVQYLYNDGRTYYFMNQSTFEQFEVPGDLVGDSAGLMKEGDGVSLMLFEG
                     RPIQVELPKNVPLKVTYTENVVKGDTSTSVTKDATLETGITIRVPAFIKVGDLISVDT
                     TNLTYRERVKE"
     gene            1110..1766
                     /locus_tag="VN778_03350"
     CDS             1110..1766
                     /locus_tag="VN778_03350"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HXR50037.1"
                     /translation="MYFASRSQAGKMLANQLVTKYRYENCAVVALDDGGVVVGAQIAA
                     SLHCPLELLLNGEITLPRELEAIAAIDQNGTVTFNSKYSPGELDELIGEYYQYIEQEK
                     LTRLRELNHLLGRGKTVDKRLLAGRNIILVSDGLGSGFAIDMAVQFLKPIPLEKIIVA
                     TPLASIQAVDRMHILADEIFCLSVIENFMPSDHYYDQHDVPPHDKVVQIIERITLNWK
                     "
     gene            complement(1747..2436)
                     /locus_tag="VN778_03355"
     CDS             complement(1747..2436)
                     /locus_tag="VN778_03355"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HXR50038.1"
                     /translation="MLATEEQTAQLVGFFRTHGVLASHRKNEYIIEPGATPPGVFYVV
                     DGLVKAYDVTRYGEENLLIIRKSHEIFPLIWALTGQERHIIYRSLKPTTSLRVDREVF
                     MNFLRHSPDTLAPLLDITIEMYRLHSERIINLEYRTVRERVISFLLTTSERFGTVHPN
                     GVKIGVQLRHQDIASSVNSSRETTTRELAYLERKGLIGKDGPYFIIRNSEQLEAHLSR
                     PLLTKPISNSA"
     gene            complement(2476..>3060)
                     /locus_tag="VN778_03360"
     CDS             complement(2476..>3060)
                     /locus_tag="VN778_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013421901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
                     /protein_id="HXR50039.1"
                     /translation="AKATLDAGTPLFADPKWHENELLRKELQLLTLKPILYVFNVDEQ
                     TLADAGRQDELRKLIAPNQAIFICAKLESELEGLDESDARELLTSYGVNESGLVQLIH
                     AAYETLGLQSYLTAGPKEVRAWTIHKGATAPEAAGVIHTDFERGFIAAQVINYDDLVA
                     AGSETAVKAAGKVATVGKEYVMQPQDIVEFRFNV"
BASE COUNT          745 a          778 c          809 g          728 t
ORIGIN      
        1 tggtgtaccg taccgcgtag tcgaatacgg ccaaaaagtc atgggccgcg gcggctcaat
       61 cgtgaatgta cgtatcaaaa gtttgctcga tggcaaggtt ctggagaaaa cctataaagg
      121 taatgagcaa atcagtggtg ccgatgtggc ctcacaaaat gtgcagtatt tgtacaatga
      181 cggtcgcacc tactatttca tgaaccaatc gacgttcgag cagtttgaag tgccaggcga
      241 cctagttggc gatagtgctg gacttatgaa agaaggtgac ggcgtcagcc tgatgctatt
      301 tgagggccgc ccgattcagg tagagctacc caaaaatgtt ccgcttaaag tgacgtacac
      361 tgaaaacgtc gtcaaaggtg atacctcgac gtccgtaacc aaagacgcga ctctcgaaac
      421 cggcatcaca atccgcgtac cggcgttcat taaagtcggt gaccttatct cggtcgacac
      481 cacaaacctg acctaccgcg aacgcgtgaa ggagtagcgt tcttttccaa gcgaatcaaa
      541 tttggtcctt tccaaggaat caaaccttct acctttgcct tgatgcaaag gtagccaaaa
      601 atcaaggccg gtgctcgcct cttgggtact gttgtaaaag cctacaagcg cctgcggaac
      661 gcctcagaaa cagttcgtct acgacgcagc tgtttcttcg ttgaacggtc ctcggcttgg
      721 ttagcttgta gtttttacga acagcccgcc aggaggctgc gctgtggcca gaaaacagcc
      781 aaggtacggt tcctgccgct attgatttag attataaaca gcagcgtaag ccgctccatg
      841 gcggctttcg ggccatttgg agcgaacctg gcgggaatgt tgtgaatacc cacaactttt
      901 cctgacgtag tcaggaatgc ggaacgactg ccccgaaggg gacgagcccg gacgctagga
      961 gggtcgctta gtggagcggt tgtggtgttt tacaaccttc acccaagtga gtgaaacggc
     1021 cttgaacttt tgcttctttt ggttcaagcc aaaagaaggt aactttgctt ctttggaaag
     1081 aactgccatt tagttgctaa actaaacata tgtattttgc gagccgatcg caggcgggaa
     1141 agatgctagc taaccagctg gttacgaaat accgctacga aaactgtgct gtcgtggcgc
     1201 tggatgatgg tggcgtggta gtcggagccc aaatcgcagc gagcttgcat tgtccgctgg
     1261 aattgttgct taacggagaa atcaccctgc cgcgtgaact ggaagcgatt gccgccatcg
     1321 accaaaacgg cacggtaacg tttaacagca agtactcgcc cggcgaactc gacgagctga
     1381 tcggcgagta ctaccagtac attgaacaag aaaaattgac gcgcttacgc gaactcaatc
     1441 acttgctcgg ccgcggtaaa acggtcgaca agcgcttgct ggccggtcga aatattattc
     1501 tggtatctga cgggcttggg agcggcttcg cgatcgacat ggcggtgcaa tttctaaaac
     1561 caattccgct cgaaaaaatc attgttgcca cgccgctggc cagcatccag gcggttgacc
     1621 ggatgcacat cttggcagat gagattttct gtctgagcgt tatcgaaaac tttatgccaa
     1681 gtgatcacta ttatgaccaa cacgacgtcc cgccgcatga caaggtcgtg caaattattg
     1741 agcggatcac gctgaattgg aaataggctt agttagtagg ggccggctta ggtgagcttc
     1801 gagttgctcg gaatttctga taataaagta cggaccatct ttgccaatta aacctttgcg
     1861 ctcgagatac gctaattccc gtgttgtagt ttcacgactg gaattcaccg agctcgcaat
     1921 atcctggtgg cgtaactgta caccaatctt gacgccattt gggtgcaccg tcccaaaacg
     1981 ctcgctcgta gtcagcagga acgagatgac ccgctcgcgc accgtgcgat actccagatt
     2041 tataatgcgt tcgctatgca ggcgatacat ttcgattgtg atgtcgagca gcggtgccag
     2101 ggtatccggc gaatgacgca ggaaattcat gaagacttcc cggtcgacgc gcaaagatgt
     2161 cgttggcttt aaactgcgat agattatgtg ccgctcctgt cccgtgagcg cccaaatgag
     2221 cggaaaaatc tcgtggcttt tacgaataat gagaaggttt tcttcgccgt agcgtgtgac
     2281 atcataagct ttgacgagcc catccacgac ataaaaaaca cccggcgggg tagcgccggg
     2341 ctcaataata tattcatttt tgcggtgcga ggccaggaca ccatgtgtcc gaaaaaatcc
     2401 caccagctgc gcggtctgct cctccgtggc cagcatatac atattatacg ggaactgatg
     2461 tgatctgaca aacgactata cgttgaaacg aaactccacg atatcttgtg gttgcatgac
     2521 gtactctttg ccgaccgtag ctactttacc ggcagctttc acggccgttt ctgagccggc
     2581 agcaacaagg tcgtcatagt tgatcacttg ggcggcgata aagccgcgtt caaagtcggt
     2641 atggatgacc cccgcggcct ctggggcggt ggcgccctta tgaatcgtcc aggcgcgaac
     2701 ttctttcggt ccagcagtta ggtaactctg cagcccaagc gtctcatagg cggcgtggat
     2761 tagctggact agccccgact cgttgacgcc gtagcttgtc aatagctccc gcgcatcgct
     2821 ctcgtcgagc ccttcgagct cgctttccag cttggcgcaa atgaagatcg cctgattggg
     2881 cgcgatgagt ttacgtaatt catcctggcg gccagcatcc gctagcgtct gctcatcgac
     2941 gttgaaaacg tacaaaatag gcttcagcgt cagcagctgc aattctttgc gcaacagttc
     3001 gttctcgtgc catttcgggt cggcaaataa cggcgtaccg gcatcgagcg tggccttggc
//
LOCUS       DATNGP010000131         4658 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_1064066, whole genome shotgun sequence.
ACCESSION   DATNGP010000131 DATNGP010000000
VERSION     DATNGP010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4658
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1064066"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(80..156)
                     /locus_tag="VN778_03365"
     tRNA            complement(80..156)
                     /locus_tag="VN778_03365"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(120..122),aa:Pro,seq:tgg)
     gene            complement(230..1024)
                     /locus_tag="VN778_03370"
     CDS             complement(230..1024)
                     /locus_tag="VN778_03370"
                     /inference="COORDINATES: protein motif:HMM:NF024674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HXR50040.1"
                     /translation="MNILYIGDVMGEMGLQVIEQVLPELRESRQIDLVIAQAENVTEG
                     RGISLEDFKRLQKTGVDFCTGGNWSLHHEDIIPMMNDLEQPIIRPANYVSGTPGLGWK
                     YVATAKGKVLVASLLGKVVGKDADKLIDNPLQVIDRILEEQKSVEHLATVVNLHGDYS
                     SEKVIIGHYLDGRATLVAGDHWHVPTADARVLPKGTAHITDVGMCGSLNSSLGITFDS
                     VVPRWRDGVQTRNVLETAGPKQFNAVLVKTSDSGLADSIVQIRQVF"
     gene            complement(1035..1313)
                     /locus_tag="VN778_03375"
     CDS             complement(1035..1313)
                     /locus_tag="VN778_03375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50041.1"
                     /translation="MSIYEAAEARDSVEQSLRDLQWLVIETLIHRKEVLDTQREFEVL
                     VKAGKVNDFTQFVYRERFRRSLRDIDDMLRLIYQQRSIAKLQLRKLVG"
     gene            complement(1525..3036)
                     /gene="rny"
                     /locus_tag="VN778_03380"
     CDS             complement(1525..3036)
                     /gene="rny"
                     /locus_tag="VN778_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HXR50042.1"
                     /translation="MMLEVVLAIVGLAVGFGANAAITKQRMGSAEEKAQKELQRAKKE
                     AEKLIDQAREEANKAVDETRKEEQARRKELKDLEQRLVTREEALDKKLDELDKRGEAL
                     RKSEDEVEQLKNEIRDIRSKQQDKLEKIAKLSKSDAADKLMQMTERDIKNDLLGLVNK
                     LQNEARDNAEEQAALIITTAMERMASEVTAERTVTSIKLEDDEMKGRIIGKEGRNIQA
                     LQRATGVDIMVDDTPGYVVLSSFDPVRREVARQAMEMLMKDGRIHPGRIEEVVEKAQK
                     NVEKDVIRAGEDAAREAGIIGIPKEILHLLGELKYRTSYGQNVLKHSTEMAHIAGMIA
                     EEVGADIKTAKYAALVHDIGKALTHKMEGKHHHISGEILRKYGAPEAVALAAEQHHDD
                     VDATSTEALIIRTVDAISASRPGARNISAENFAERMKDLENVANSFPGIDKSYAISAG
                     REVRVFVKPESIDDLSAIKLARDIATKIESTMQYPGTIKVNVIRETRAVEFAK"
     gene            complement(3247..3639)
                     /gene="tsaB"
                     /locus_tag="VN778_03385"
     CDS             complement(3247..3639)
                     /gene="tsaB"
                     /locus_tag="VN778_03385"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HXR50043.1"
                     /translation="MLILTIRTDKPEAEIGLFHSTTQLAYLTWPAHRELAETIHLKIK
                     ELLTSQAKELYHLQGVIVFKGPGSFTGLRIGVSVANALADGLNIPIAAVSGDKWIQVG
                     IVQLNDGEGQKLAVPEYGAPPHITRPKH"
     gene            complement(3652..4023)
                     /gene="tsaE"
                     /locus_tag="VN778_03390"
     CDS             complement(3652..4023)
                     /gene="tsaE"
                     /locus_tag="VN778_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008578991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HXR50044.1"
                     /translation="MIELISDLGGGKTSFVRGLAQGMGSDDSVHSPSFTLSNEYVGGK
                     LTLHHLDFYRLQEPGIMREELAEMLCDLKAVVVVEWADIVENVLPINRLRIRIKATGD
                     TSREFTLIYPESLDYLIYKET"
     gene            complement(4040..>4658)
                     /locus_tag="VN778_03395"
     CDS             complement(4040..>4658)
                     /locus_tag="VN778_03395"
                     /inference="COORDINATES: protein motif:HMM:NF013125.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HXR50045.1"
                     /translation="FIELYNPNDVSFDLSDFSLQTGTTTLHEYTFPAGTSLPSKSYTA
                     YYSAITKLSLSNSGGQVKLLDPYGNAVYATAAYDTANDGLTWALANGNWYWTTKPTAG
                     AGNVMMQPPAQKSSGKQNAAAKAKSSKSKTAKLKSKKLKATKTKATLASNNSSTTPTT
                     PIHASILALVAGLALLYGAYEYRTDIKNRVYQLRRYFRVRAADRL"
BASE COUNT         1024 a         1212 c         1125 g         1297 t
ORIGIN      
        1 gcaaaaaaca gaggtcagag gtagttgcaa aattaaatga accctgggat gggttcataa
       61 aaaccaacaa tttcgttgat ggtcggggtg agaggacttg aacctccggc ctccgcgtcc
      121 caaacgcggc gcgctagcca actgcgccac accccgttgc tttcgggctc tacccaggca
      181 gagcaacagg ggtcattcta gcatgtttta gctactaaag ccagtagagc tagaacactt
      241 gtctgatttg cacgatggag tctgctaatc cgctatcgct ggtctttacg aggacggcgt
      301 tgaattgttt aggaccggca gtttcgagga cattacgagt ttgaacgcca tcacgccagc
      361 gggggacaac gctgtcgaag gtaatgccta gcgaggaatt aagcgagcca cacatgccga
      421 cgtcggtgat gtgagccgtt cctttaggta aaacgcgggc atcagctgtc ggcacgtgcc
      481 aatggtcgcc agcgacgaga gtggctctgc catctagata gtggccgata atgacctttt
      541 cgctcgaata atctccatgc aaattgacga ccgtggcgag atgctcgacg gatttctgtt
      601 cttctaaaat tcgatcgata acttgcagcg gattatcgat gagcttgtcg gcatctttgc
      661 cgacaacttt gccgagtaag ctagcaacta gaacctttcc tttggccgtg gcaacatact
      721 tccaacctaa gcctggggtt cctgacacgt aatttgctgg tcgaattatt ggttgctcca
      781 ggtcgttcat cattgggatg atatcttcgt gatgcaagct ccagttgccg ccggtacaaa
      841 aatccacgcc tgttttttgc aaacgcttaa aatcctctaa gctgataccc cgtccctcgg
      901 tcacattctc ggcttgggca ataaccagat cgatctgccg actttcgcgc aactcaggca
      961 gcacttgttc aatcacttgc aggcccattt cgcccattac gtcgccgatg tatagaatat
     1021 tcattactta aagattaacc cactaacttt ctgagctgaa gtttggcgat acttctttgt
     1081 tgatagatga ggcgtagcat gtcatcgatg tcgcgcagtg accgccggaa gcgttcgcgg
     1141 taaacaaatt gcgtgaagtc gtttacttta ccggctttaa ccaatacttc aaactcgcgt
     1201 tgtgtatcaa gaacctcctt gcgatggatt agcgtctcaa taacgagcca ttgcaaatcc
     1261 ctgagcgact gctccacgga atccctagct tctgctgctt cgtagatgct cattcgtttc
     1321 cgatctaata ttctagcttc aagccaattt tttgagcgat ctggtgtatc cacttttcca
     1381 tgcgctcaaa ttgatattcg cgagccagca cttcttaaat cggccagatc ttgcttggtt
     1441 gccggttgca tacttgtact ttacgccaag ccccacgtct tgaataggca atttaaggaa
     1501 aatttaagaa gtaaatttac aaagttactt agcaaattca acggcgcggg tttcgcggat
     1561 gacattcact ttgatcgtgc ccgggtattg catcgtggat tcgatcttgg tggcgatgtc
     1621 gcgcgccagc tttatggcgc tgagatcatc aatagattca ggcttcacga aaacccggac
     1681 ttcacggccg gcactaatgg cgtatgattt gtcgatgcca gggaagctat tggccacgtt
     1741 ctcgaggtct ttcatacgtt ccgcaaaatt ctcagcactg atgttgcggg cgccaggacg
     1801 cgaagcgctg atagcatcaa ccgtgcggat gatgagggct tcggtactgg tagcgtcaac
     1861 gtcgtcatga tgttgttcgg cagccaaagc gactgcttct ggggcaccgt atttgcggag
     1921 gatttctccc gaaatgtggt ggtgcttgcc ttccattttg tgtgtcaaag ctttgccgat
     1981 atcatgcacc agcgccgcat acttggccgt tttgatgtcg gcgcccactt cttcggcgat
     2041 catgccggcg atatgtgcca tctcggtact gtgctttagt acattttggc cataactggt
     2101 gcgatatttc agctcaccta gcagatgcag aatttctttt ggaataccga taataccagc
     2161 ttcgcgggcc gcgtcctcgc cggctcggat gacatctttt tcgacattct tttgagcctt
     2221 ttcgaccact tcttcaatac ggccgggatg gatgcggcca tctttcatga gcatttccat
     2281 agcttgtcgg gctacctcgc ggcgtaccgg atcgaagctg ctgagcacaa catagccggg
     2341 ggtatcgtca accatgatgt cgacgcccgt ggctcgttgc agcgcttgga tgttgcggcc
     2401 ttctttgccg atgatacggc ccttcatctc atcgtcctcc agcttgatac tggtaaccgt
     2461 gcgctcggcc gttacttcac tggccattcg ttccatggcc gtcgtaatga taagagcggc
     2521 ttgctcttcc gcattatcgc gagcttcgtt ttgcaattta ttgaccagtc caagcagatc
     2581 atttttgatg tcgcgctcgg tcatttgcat cagcttgtcg gcggcatcgg acttgctgag
     2641 cttggcaatc ttttctagct tgtcctgttg cttgctgcga atgtcgcgaa tctcattttt
     2701 cagctgctcg acttcatcct cacttttacg caaggcctcg cctcgtttat cgagctcgtc
     2761 aagctttttg tcgagcgctt cttcacgggt aaccagccgc tgttcgagat ccttcagctc
     2821 tttgcgacgc gcttgctctt ctttgcgcgt ctcatcgacg gccttattag cttcttcgcg
     2881 agcctgatcg atcagctttt cagcctcttt tttggcgcgt tgtaattcct tttgggcctt
     2941 ttcctcagct gagcccatgc gctgtttcgt gattgctgca tttgcaccaa aaccaacagc
     3001 tagccccact atagcgagca ctacttctaa catcatgatc ctttctattc ctgtagaacg
     3061 gctccgcctg aaggcgtcgc tttcacttct ctagcctgtc agtaaccgtt ctgaaactcg
     3121 aattgattaa aagattccag aggatctgca ccgctctcaa actgtaaaac gtggcaattc
     3181 ctgtaactcc aatatacaac gacgcttcgt tcgtgtctac aaaacatgtg ccggcatgct
     3241 atggaatcag tgttttggtc tagtaatatg cggcggagcg ccatactcag gcacagctag
     3301 tttttggccc tccccgtcat ttaactgaac tatgcccacc tgtatccact tatcgccgct
     3361 caccgcagca atggggatat ttaacccgtc ggctaaagca ttggcaacgc tcacgccgat
     3421 gcgcaaaccg gtgaagctgc cgggcccctt gaaaacgatt actccctgca ggtggtacag
     3481 ctctttggcc tgagaagtta acagctcctt gatctttaga tggatcgttt cggctagttc
     3541 tcggtgagca ggccacgtta ggtaggccag ctgagtagtg ctatgaaata aacctatttc
     3601 ggcttcaggt ttgtcggtac ggatagttag tatgagcata tagttaactt gttaagtttc
     3661 cttatagatt aaatagtcta gactctcagg atatatcaat gtgaactctc tactggtatc
     3721 acccgtggcc ttgatacgaa ttcttaatcg gttaataggt aagacatttt cgacaatgtc
     3781 cgcccactcg accacgacga cagcttttaa atcacaaagc atttcagcca gctcctcgcg
     3841 cataataccc ggctcttgca atcgataaaa atcaaggtgg tgcaaggtaa gtttgccgcc
     3901 cacgtattca ttactgagag taaaacttgg gctgtgtacg ctgtcatcgc tccccatgcc
     3961 ttgggccaag ccacgcacaa aactagtctt gccacctccg agatcactga tgagttcgat
     4021 cacttcgcca cctctgagcc tacaaccgat ctgccgcgcg aactctaaag tagcgtctaa
     4081 gctgatagac tcgattttta atatccgtcc ggtattcata cgctccgtat agtagcgcga
     4141 gcccggcaac taaggcaaga atggaggcat gaatcggtgt tgtcggcgtg gtcgaggagt
     4201 tgttactagc gagggtagct ttggttttgg tggccttcag cttcttgctc ttgagcttgg
     4261 cggtttttga cttgctcgac ttagccttag ctgctgcgtt ctgtttgccg gaagacttct
     4321 gagccggtgg ctgcatcatc acattacccg ctccggccgt cggcttggtc gtccaatacc
     4381 agttgccatt ggccagcgcc caggtcaggc catcgttcgc ggtgtcataa gccgccgtgg
     4441 cgtagaccgc gttgccataa ggatcaagca gcttgacctg gccgccgctg ttgctcaggc
     4501 tcagcttagt aatcgccgaa taataagcgg tgtagctttt tgatggcaaa ctggtgccag
     4561 ctgggaaagt atattcatgg agggttgtcg taccggtctg cagactgaaa tcactcaaat
     4621 caaaactaac gtcattcgga ttataaagtt caataaat
//
LOCUS       DATNGP010000132         7194 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_3492913, whole genome shotgun sequence.
ACCESSION   DATNGP010000132 DATNGP010000000
VERSION     DATNGP010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 7194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7194
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3492913"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            155..228
                     /locus_tag="VN778_03400"
     tRNA            155..228
                     /locus_tag="VN778_03400"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:187..189,aa:Cys,seq:gca)
     gene            234..644
                     /locus_tag="VN778_03405"
     CDS             234..644
                     /locus_tag="VN778_03405"
                     /inference="COORDINATES: protein motif:HMM:NF025793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deaminase"
                     /protein_id="HXR50046.1"
                     /translation="MSDEIFLKRAVHVATHSREPVKCGVVLVNEAGEMIAQAYNSQRD
                     DEQVVNHAEIKALQEANHILNSRKLKGVTAYCSCEPCVMCLVALSLADVERVVFTETM
                     RDVDPESSLSQVDAREFVNRYLRGIPKLERLVVS"
     gene            658..745
                     /locus_tag="VN778_03410"
     tRNA            658..745
                     /locus_tag="VN778_03410"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:692..694,aa:Leu,seq:taa)
     gene            939..1015
                     /locus_tag="VN778_03415"
     tRNA            939..1015
                     /locus_tag="VN778_03415"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:973..975,aa:Ala,seq:cgc)
     gene            complement(1003..1542)
                     /locus_tag="VN778_03420"
     CDS             complement(1003..1542)
                     /locus_tag="VN778_03420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HXR50047.1"
                     /translation="MGDTRVPIIETCVITRISIASRPIDVFKYLVNLKYHLIWIPHLQ
                     SISPLTKLKLGTVYKSTILLLGLIKVNGQNLVTKLVPDRELAIENKIGPLQYNVRWFL
                     RPKNDSTILICTAHVTADMHTFGFVSPELNHLVRTELRTELRSLKKLVEHGGVEPKKP
                     PAELFVDSSEAPLKKHGGA"
     gene            1658..3562
                     /locus_tag="VN778_03425"
     CDS             1658..3562
                     /locus_tag="VN778_03425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50048.1"
                     /translation="MDPNTNNPEQLPPTPPIPPPQPPVGSQPSAPAPGFTQPMPGQTI
                     SPGTVSGPAAVSGSFNTPPLAPAPVAPSPNQQPVLPTPDAGNTSNQLPLTPPGRRSFK
                     PLILILVLILVLIGSAAAYYFGYYMNSSVILSQSLSDTGKGYNSLINYFDSQSKVSYT
                     GSTGQGSYSIKTDSFTTDGSLSFNSNGENSDATIDVGYGTGRVKGEVRTVKAASGTEP
                     DVYIKASGISGLGSLLGSPELSSALNQYDGKWLQVDHTLLENLSNASNPTASLTQNEN
                     LPSRDQVVDELQAFGNVSQQYVFTTNKDKSVTTVVKKYGVESVDGHKAYHYTMALDKS
                     HVKQYISAQQAALKNSKLDAWLKKNNLEADVDSSFKDMQSSADKIKSSDTFELWADVK
                     TRLVYKVRINDKTAKNPAANYVDIGLNYKGGPSYPFFISGQTKDSSSSTNVNLVVTLD
                     SKTNSAGLKVDFKSTGDVPSSGTVNFTFKPTNSVQTISAPTGAVPLSQVLSQLGLSDL
                     LTQAVSSANPTASSGNAKDAKRQSDIQALQTQLEAFFSQNGYYPNLADINNATWRQTN
                     MQSLDSNSLQDPDSSSKTLAATPAAKVYAYKVTNSSGGSCESDDTSCAKYALTATLSN
                     GTTDVVNNLD"
     gene            3586..4863
                     /gene="murF"
                     /locus_tag="VN778_03430"
     CDS             3586..4863
                     /gene="murF"
                     /locus_tag="VN778_03430"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HXR50049.1"
                     /translation="MDTVKKIGKAFLCHLLEAQVKRLQQKNHFKVIAVAGSVGKTSTK
                     LAIARTLSSRERVIFQGGNYNDRLTVPLVLFGQVEPSIFNVFAWLNILAANERLLRRN
                     YPYDTAVLELGTDEPGQLERFAYLQPDLVVITAVAAEHMEFFKSLEAVAKEELTPLGF
                     SQQALLNIDDIDAQYFSDTAFVSYGYQTQADYRLVEHRQHGLEGQELEISLDGGQKLT
                     IQTHLLGAQGHKIILAAAATAHILGWEPSAISEGLHHITPTPGRMQILPGIKESHLID
                     DTYNASPIAVRAALDVLYSIEAPQRIAILGSMNELGTASAEAHEQIGAYCDPKKLDLL
                     VTIGSEAEKHLAPAARAVGCEVVSRKSPYEAGEYVKQHLQAGAVVLAKGSQNGVFAEE
                     ALKLLLRKPDDATRLVRQSAYWLTVKRRQFPNA"
     gene            complement(4853..5758)
                     /locus_tag="VN778_03435"
     CDS             complement(4853..5758)
                     /locus_tag="VN778_03435"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF002378.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HXR50050.1"
                     /translation="MSQVLILAGGDSDEREVSLRSGAAVKNALEQAGYQVSALDPADG
                     LDKQLAALKKADVVFPALHGKGGEDGTLQRFLEKHDLCYVGADSKASALCFDKARYTA
                     LLKQQAALVPDTKLVNYAEYQGSELTRAPHVLKPNDGGSSIDTFIVRDPAEVDEQAVA
                     ETFSRHQQLLLQALIPGVEITVAVLNAQSLPVIEIIPPANQEFDYENKYNGATQELCP
                     PLHVSEALQKKAQALAKRIHVLTGCRDMSRTDIIISDSGELYVLETNTIPGLTDQSLL
                     PKAAAAAGYDMAGLCDQLVQAALKH"
     gene            5834..>7194
                     /gene="leuS"
                     /locus_tag="VN778_03440"
     CDS             5834..>7194
                     /gene="leuS"
                     /locus_tag="VN778_03440"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HXR50051.1"
                     /translation="MQRYNPRDIEPKWQQTWEDSKIYQASEDPAKPKKYVLEYFPYPS
                     GAAMHVGHVRNYTIGDAIARFNRMNGYNVLHPMGWDAFGLPAENYAIKNKISPRKAID
                     ENTTRFRQQLMQMGFSYDWSREIDSTDPKYYKWTQWFFLLLFKRGLAYQQESLQWWCP
                     HDKTVLANEQVEDGRCWRCGHLVEKKSLKQWFFKITDYADRLLDDLDDLDWSDSIKTM
                     QRNWIGRSDGVVYTNEVRNTDLQIESFSAHFEAFTANTFVVIAPDHPFLPTLLEGLDK
                     KDVILAAAQKMALQRDIEGPKGLEHVEGIFTERYAIDIFGDKDLPIWIANYALADYGT
                     GIVLCSAHDERDFTFAKKYGLRLKPTMVPTDPVEAEKVKNLEYCFTDMKNGILIEPKE
                     FEGKVAGGNRQEIARYMEHEGYARTEISYKIRDWLISRQRYWGAPIPIIHCPNDGAVA
                     VPD"
BASE COUNT         1848 a         1792 c         1823 g         1731 t
ORIGIN      
        1 ggagcagatg cgcatgaggc cttccttcat ccatatgctt acgcgcccag agctgctgaa
       61 gtaagcgaac tacatagcca atagttcatc gtacggggga atggaaacca gccacaccac
      121 ctatcttttc atggtagact gataccagta atttggcgcg ttggccgagg agttaggcaa
      181 gggtctgcaa aaccctgtac acgagtgcga atctcgtacg cgcctccagt tttatgagtg
      241 atgaaatttt tctgaaacgg gcggtacatg tagcaactca ttctcgtgag ccagtaaagt
      301 gcggggtggt tttggttaat gaagcaggtg agatgatcgc ccaggcttac aattctcaga
      361 gagacgatga acaagtagtc aatcatgcgg aaatcaaagc actccaagaa gcgaatcata
      421 tactcaacag ccgtaaattg aaaggtgtaa cagcgtattg cagctgcgag ccgtgcgtta
      481 tgtgtctcgt ggctttatct ttagcggatg ttgagcgcgt ggtatttact gaaactatgc
      541 gagacgttga tcccgaaagt tcattatcac aggttgacgc tcgtgaattt gtaaatcgtt
      601 accttcgcgg tattccgaaa cttgagcgtc tagtggtatc ataaccgaag tacttacggg
      661 cgagtggcgg aattggtata cgcggcggac ttaaaatccg ctggctgaat aagccttgag
      721 ggttcgagtc cctcctcgcc caccaggact ttaacacctt actgttatcc accatacatt
      781 ttctgtacag aaaatgtaca taaaatgtat aggcctacat ttaggagtgc gcactgaaga
      841 ctgttcgaaa ggacacttcg ttcgcgtcta cagctctgac gtagttgcac taaggacact
      901 tcgttcgcgt ctacataagt gcccacctgg gcacttatgg ggcattagct cagatggcta
      961 gagcgcggca ttcgcattgc cgaggtcagg agttcgactc tcctatgctc caccatgttt
     1021 tttcaagggc gcttcgctcg agtctacaaa gagctccgcc gggggctttt tgggttcgac
     1081 tccgccatgc tccaccagtt tttttagaga tcgcagctcg gtccgcagct cggtccgaac
     1141 tagatgattc aattccgggg acacgaagcc gaaggtgtgc atatcggccg taacatgagc
     1201 ggtgcaaata agtatggtcg agtcattctt aggtcgtaaa aaccagcgga cattgtattg
     1261 tagcggacct attttattct ctatggccaa ttctcggtca gggacgagct tggtaaccag
     1321 attttggccg ttgaccttta ttaggcctaa cagtaggatg gtgcttttat ataccgtgcc
     1381 taacttcagt ttggtaagcg gtgaaatgct ttgtaagtga ggtatccaga tgagatggta
     1441 cttcaggttc actaaatatt tgaagacgtc tatcggccga ctggctatag aaattcgggt
     1501 tataacgcag gtttcaatta tcggcaccct agtatccccc atctttaatt atacgcctga
     1561 ctaccagggg ttgatatggt tggggaattt ataacactta tgtataatca agcacagaga
     1621 tcaaaacaaa aactactgct taaggatcag tgatctgatg gaccccaaca ccaacaaccc
     1681 tgaacaactt cctcctacgc cacctatacc tccgccacag ccgcccgttg gcagtcagcc
     1741 gagtgcgccg gcgccaggct ttacgcagcc catgcccggt cagacgatat ctcccggcac
     1801 ggtttccggc cccgcagccg tcagcggttc gtttaacaca ccgcccctgg cacctgcgcc
     1861 cgtggccccc tcgccgaatc aacaaccggt gttgccgacg cccgatgccg gcaatactag
     1921 caaccagctc ccacttaccc ctccaggccg tcgctctttt aagccgctca tactgatttt
     1981 agtcctgatc ctcgtgctca ttgggagtgc tgccgcctat tatttcggct actacatgaa
     2041 ttcgtcggtt attttatcgc agtcgctcag tgacaccggc aagggctata actctctgat
     2101 caactatttt gacagtcagt ccaaagttag ctacacaggc tcgaccggcc aaggttcgta
     2161 cagcattaag actgacagct ttactactga cggcagcctg agctttaact ctaacggcga
     2221 gaactctgac gccactattg atgtaggcta cggcaccgga cgcgttaaag gcgaagtgcg
     2281 tacggtaaaa gcagccagcg gcaccgagcc ggatgtatat attaaggcca gcggtatcag
     2341 cggcttgggc tcgctcctcg gatccccgga gctgagtagt gcgctgaacc agtacgacgg
     2401 caagtggttg caagtcgacc ataccttgct cgagaatttg tcgaatgcgt ctaatccaac
     2461 cgctagctta acgcagaatg aaaacttacc gagtagggat caggtagttg acgaattaca
     2521 ggctttcggt aatgtcagcc agcagtatgt ctttaccaca aacaaggata agtcagttac
     2581 caccgtagtg aaaaaatatg gcgtggaatc ggtcgacggc cataaagctt accactacac
     2641 catggcgctg gacaaaagcc acgtcaagca atatatctcg gcccagcaag cagccctgaa
     2701 gaacagcaag ctcgatgcgt ggctcaaaaa gaacaaccta gaagccgacg tcgattcgtc
     2761 atttaaggac atgcaatcgt cagctgacaa gatcaagagc agcgacacct ttgagctatg
     2821 ggccgacgtt aaaacacggt tagtttacaa agtgcggatt aacgataaaa ctgctaaaaa
     2881 tccggctgcc aattatgtcg atatcgggct gaattacaaa ggtggcccga gctacccctt
     2941 cttcattagc gggcagacca aggatagcag cagttcgacc aatgtcaact tggttgttac
     3001 gcttgattcc aagaccaata gcgctggcct gaaagtggac tttaagagca cgggcgatgt
     3061 acccagtagc ggcaccgtta atttcacttt caaaccgacc aacagtgtcc aaactatctc
     3121 agcgcccact ggtgcggtgc ctttaagcca agtcttaagt caactggggc tcagtgattt
     3181 gctaacccaa gctgtgtcaa gcgctaaccc aaccgcgtca tctggcaatg ccaaagacgc
     3241 taaaagacaa tcagatatcc aagcgttgca aactcagctg gaagcgtttt tcagtcaaaa
     3301 tggttactat ccaaacctgg ctgatatcaa taacgccact tggcgacaga cgaacatgca
     3361 aagcctcgat agtaatagtc tgcaagatcc tgatagctca tcgaagacgc tggcagccac
     3421 accagcagct aaggtgtacg cctataaagt tactaattcc agcggcggca gctgcgagag
     3481 tgacgacacc tcatgcgcca aatacgcttt gaccgccacc ctgtctaacg gcacgacgga
     3541 cgtcgtcaac aatctggatt agctccctga tatactaaag atcatatgga tacggtcaaa
     3601 aaaattggca aagcttttct gtgtcatcta cttgaagcgc aagtcaaacg attgcagcaa
     3661 aagaaccatt ttaaagtgat tgctgtcgcc gggagcgttg gcaagacctc tactaaatta
     3721 gcgattgcac gaaccctcag ttcccgagag cgagttattt tccaaggagg gaattataac
     3781 gaccgtttga cagtgccgct agttctgttt ggccaggtgg agccgagcat tttcaatgtt
     3841 ttcgcttggc tgaacatctt agcggctaat gagcgccttt tacgcaggaa ctatccttat
     3901 gacactgctg ttttggaatt agggacggat gaaccgggcc agctggagcg cttcgcttat
     3961 ttgcagcctg acctagtggt tatcacagcc gttgctgccg agcatatgga gttcttcaag
     4021 tcgcttgaag cggttgcaaa agaggagtta acgccgttgg gcttttctca gcaggccttg
     4081 cttaacatcg acgacattga tgcgcagtat ttttcggaca cggcctttgt gagctatggc
     4141 taccaaacgc aggctgacta tcggcttgtc gaacatcgtc agcatggtct cgaagggcaa
     4201 gagctggaaa taagcttgga cggcgggcaa aagctgacta tccagacgca tttgctcggt
     4261 gcacaggggc ataagataat cctagctgcg gctgccacgg ctcatatatt aggttgggag
     4321 ccgtcagcta tttcagaggg gctgcatcac atcacgccaa cacccggccg catgcaaatc
     4381 ctgcctggga tcaaagagtc tcaccttatt gatgatacct acaacgcgag tcctatcgct
     4441 gtccgagcgg cgctggatgt gctttatagt atcgaagcgc cacagcgtat agcaattttg
     4501 ggcagtatga atgagcttgg tactgccagt gccgaagcgc acgagcaaat tggcgcctac
     4561 tgtgatccta aaaagctgga tttgctcgta acgattgggt cggaggctga aaagcatcta
     4621 gcacctgccg cgagagctgt tggctgtgag gtggtgagtc gcaaaagccc gtacgaggcg
     4681 ggagagtacg ttaagcaaca cctgcaagct ggtgctgttg tcttggcgaa aggttcgcag
     4741 aatggtgtat ttgccgaaga agccctcaag ctattgttac gaaagcctga tgatgccact
     4801 agactggtgc gccaatcggc gtactggcta actgttaagc ggcgacaatt ccctaatgct
     4861 taagtgccgc ctgcaccagc tgatcgcaca aacccgccat gtcatagccg gccgccgcgg
     4921 cagctttggg cagcaagctt tgatcggtta agcccgggat ggtattggtc tccagcacgt
     4981 atagctcgcc ggaatcgctt ataatgatgt cggttcggga catatcgcgg caccctgtca
     5041 aaacatgaat ccgcttggcg agcgcctgag cttttttctg caacgcttca ctaacatgca
     5101 ggggtgggca taattcctga gtcgccccgt tatatttgtt ctcataatca aattcttggt
     5161 tagccggggg aatgatctcg atgaccggca aactctgagc gttgagcacg gcgaccgtga
     5221 tctccacccc tgggatcaag gcctgtaaaa gtaactgctg gtgacgcgaa aatgtctcag
     5281 caacggcttg ctcatcaacc tctgccggat cacgcacaat gaaggtgtcg atgcttgatc
     5341 cgccatcatt cggcttcaat acgtgtggcg cgcgcgtcaa ctccgacccc tgatattcgg
     5401 catagttaac aagtttagta tccgggacta gagcagcttg ctgtttaagt aatgcagtat
     5461 aacgggcctt gtcaaaacac agggccgaag ccttgctgtc ggctccaaca tagcatagat
     5521 catgtttttc tagaaagcgt tgcagcgtcc catcctcgcc acccttacca tggagggcgg
     5581 gaaaaactac gtcggctttt ttcagggcag ccagctgctt gtctagtccg tcagccggat
     5641 caagcgcact aacttggtag cctgcttgct cgagagcgtt cttaactgcc gctccggacc
     5701 gcaaagatac ttctcgttca tcagaatcac cacctgcgag aattaagacc tgggacatgc
     5761 tctgattata ccgaacattt aaccggtgct gcatttccgt tataatttta gtaagacaac
     5821 aggagtttta agcgtgcaac ggtataatcc gcgagatatt gagccgaaat ggcagcagac
     5881 ctgggaagac agcaagatct accaggctag cgaagatccc gccaagccta aaaagtatgt
     5941 gctggaatat tttccgtacc cgagcggtgc cgctatgcat gtcggccatg tgcgcaacta
     6001 taccattggc gatgccatcg ctcgctttaa ccgcatgaat ggctacaatg tgctgcatcc
     6061 gatgggctgg gacgcgttcg gtttaccggc cgaaaactat gccatcaaga ataaaatcag
     6121 cccccgtaaa gcgattgacg aaaacaccac tcgctttcgc caacagctca tgcaaatggg
     6181 cttcagctat gattggagcc gggagatcga cagcactgat ccgaaatatt ataagtggac
     6241 acagtggttt ttcttgctgc tgtttaagcg cggtttagcc tatcagcaag aaagtttaca
     6301 gtggtggtgt ccccatgata aaacagtttt ggctaatgag caggttgaag atggccgctg
     6361 ctggcgctgc ggtcacctag tggagaaaaa atcattaaaa cagtggtttt tcaaaataac
     6421 tgattatgcc gaccggctgc tcgatgatct ggacgatctg gactggtctg attcgatcaa
     6481 aacaatgcag cggaattgga tcggccgtag cgacggcgtc gtgtatacca acgaggtgag
     6541 aaataccgac ctacagatcg agtctttttc ggcccacttt gaagctttta cggccaacac
     6601 ctttgtggtt atagcgcccg atcacccttt tctaccgacg ctcttagagg gcctagataa
     6661 aaaagatgta attttggcag cggctcaaaa aatggcgctg cagcgggaca ttgaaggccc
     6721 aaagggctta gaacatgtag aaggcatttt tactgagcgt tacgccatcg atatatttgg
     6781 ggacaaagat ttgccgattt ggatcgctaa ctacgcccta gctgattacg gtaccggcat
     6841 tgtgctctgt tcggcgcatg atgagcgtga ttttactttt gctaagaaat acggcttacg
     6901 tctgaagcca accatggtac cgactgatcc ggtggaggcc gagaaagtaa aaaatctaga
     6961 gtactgtttt acggatatga aaaacggcat cttaattgaa ccgaaggagt ttgaaggtaa
     7021 agtcgccggc ggtaaccgcc aagaaattgc ccgatacatg gagcatgaag gctatgctcg
     7081 gaccgagatc agctataaga tacgcgactg gttgatcagt cggcaacgct actggggtgc
     7141 gccgattccg atcatccact gcccgaatga tggagcggta gccgtacctg atga
//
LOCUS       DATNGP010000133         4925 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_369781, whole genome shotgun sequence.
ACCESSION   DATNGP010000133 DATNGP010000000
VERSION     DATNGP010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4925)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4925
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_369781"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            388..726
                     /locus_tag="VN778_03445"
     CDS             388..726
                     /locus_tag="VN778_03445"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HXR50052.1"
                     /translation="MGYSSFDHFFMTLGNKQRVRTLQLLDIEGPQSVLSIAKALNAEQ
                     SAISHSLRQLLQCHFVTVKQVGKERIYSINEDTVAPLFKQIRKHVEKYCSEGCEHWES
                     VKEVLYGHKR"
     gene            710..1423
                     /locus_tag="VN778_03450"
     CDS             710..1423
                     /locus_tag="VN778_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015596995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXR50053.1"
                     /translation="MVTNVKLLKKETVAKDTVAFYFEKPEGFEYRAGQFSDYTLIDPP
                     ETDEEGNTRGFSLITAPYEDNIGAATRLRDTAFKKVLKDLPIGTEVKLDAPYGDFTLH
                     KTETTPAVFIIGGIGVTPVRSMISQATHDKTAHKMTLLHANRTPADAPFAADFERLAK
                     ENPSFTYVPVATNTSVTDWQGERGHIDADMIKRHVSDLNVPIYYLSGPEGMVKAMRQL
                     LVSMNINEDNIRTEEFTGY"
     gene            1425..2405
                     /locus_tag="VN778_03455"
     CDS             1425..2405
                     /locus_tag="VN778_03455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50054.1"
                     /translation="MAGEFLSAESDLKRAIADDLYRNPTLIPERGVKIELASDDYADP
                     VADLYRREYERGDYFASRYDDPYEQIFNPEWLKQEFQDPEHLRFVFTDDEGKLLGATG
                     FFHDGDSESGPLMTSDATQIDPNGRGMRIMDHFFKRVVPLIEASGAGLATDFVLTAES
                     KGLRRTLQTDLGMVATGIHPHALHHRKLGITRSEISAVKNSRLIPKHAQILPFFEPLY
                     RIVQSQLPQLPEPEIIPSQPQIRSSMFIDCDSEVVKTVSGVDPQGQLEPLEAGYLPVE
                     FDPDGNRFKVAQYPRICPDLNFVLNNEQVEANKLLVRYLYDVLFATYQLN"
     gene            2424..3368
                     /locus_tag="VN778_03460"
     CDS             2424..3368
                     /locus_tag="VN778_03460"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXR50055.1"
                     /translation="MSGAESSSFSFENKNALVTGASKGLGEQIALELLQRGVHVIGAS
                     RTGPPESFQPYIEQVQADYELGDLAREGDRVISEIYDKHHGYEIFVNNAGAFSPDYFT
                     HLDPAKIRGDIELDLITPLLLHHSWFDLYNKFHPSAKTPELSVNICSISSFYAWPGGT
                     AYQAAKTGLGAAIYGLRSMQQHLNEQATDEVKQQVGPSADLATRIVAIYPDNVATGLL
                     SRAGQESLYKVQGDALPPDIVVQTVMKAIEGQGKFSEYDDVAILVNPNEPGSRKRLRG
                     VYLGFIPADEETHRPNFSNRILEKIAEEDVLIKREADS"
     gene            3365..3706
                     /locus_tag="VN778_03465"
     CDS             3365..3706
                     /locus_tag="VN778_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HXR50056.1"
                     /translation="MIKIKRVYWEYDPKDGKRILIDRLWPRGLSKDKARIDLWLKDIA
                     PTAELRQWFGHDPGKWPEFQKRYESELDANKESVETLRNQLEDGPATLVYGAKDEQHN
                     DAVVLAEYLEK"
     gene            3714..4625
                     /locus_tag="VN778_03470"
     CDS             3714..4625
                     /locus_tag="VN778_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012901164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlkA N-terminal domain-containing protein"
                     /protein_id="HXR50057.1"
                     /translation="MHEATLTLPVKPPFRLDLTVWVLRRRAKNSIDTWDGNTYRRVFT
                     IDGRPALAKVHQKTGDLVVKLKSSGTITYKRRTELEKLVRRTLGLDVNLEPFYKLTAT
                     DQTLESLTRQFVGVRPPRFPSIFETLVNAIACQQVSLNVGILLINRLADSFGKPIVDG
                     EMTLHAFPEPQDIVVVDESSLKQLGFSYQKVRAISSLAYAIANNDVDLKALSAASNDQ
                     VMAVLQSLRGIGRWSAEYVLLRGLGRINVFPGDDIGGQNNLQRLLGLDMRPSYEELRR
                     LTSKWNPYAGLIYFHLLLGKLREKELI"
     gene            4622..>4925
                     /locus_tag="VN778_03475"
     CDS             4622..>4925
                     /locus_tag="VN778_03475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50058.1"
                     /translation="MNQSISQNDSKLEFLARQSEVVIFCTTTAFRFRPERYELIIDRA
                     KITVIKRLFLLADNITSIGFGDILDVNLSVGPFFGTLTLKARYLDQPIQISPLLRDK"
BASE COUNT         1399 a         1081 c         1159 g         1286 t
ORIGIN      
        1 tcgacaatgt tagaaccacg ctagtgctat catgaaagcg tgaaaattag cctttacgtc
       61 atactaatga ttgctctcgt gttcgctgtg gatctacttt tcttccgaca ccatttccgc
      121 aaacgcttga taacgaatac aggaatcgtt ttggcttttt tagtttttta cttagtattt
      181 ttgaagcata aataaagagt aggcccaaca aaataatttg gcagagcata ccggtcagga
      241 accttttcgg cttctgttcg acaatttatg ctgatttagc ctaacactta aaaatgagac
      301 attttatagc cctataatta tcctccgata taagcagggt gtaatcaaaa ttacagaact
      361 attaaactca tatgatacaa tattcatatg ggatattcat catttgatca tttctttatg
      421 actctcggca acaagcaaag ggtgaggacg ctccagttgc ttgatataga aggcccacag
      481 agcgtgttat ctatagccaa agcactaaat gcagagcaat ctgccatttc acacagccta
      541 aggcaattgc tgcaatgtca ttttgtaaca gttaaacagg ttggtaagga acgaatctac
      601 tcgattaacg aggatacggt ggcaccgctt tttaagcaga ttagaaagca tgttgaaaag
      661 tattgctcgg aaggctgtga gcattgggaa agtgtgaagg aggttttata tggtcacaaa
      721 cgttaaatta cttaaaaaag aaactgtcgc aaaagacact gtggccttct attttgaaaa
      781 gcctgaaggc tttgagtatc gtgctggcca gttctctgac tacacgctta tcgatccacc
      841 ggaaacggat gaagaaggta atactcgagg gttttcgctt atcactgcac cgtatgaaga
      901 caacatagga gctgctactc ggctgcgtga cacggctttt aagaaggtgt tgaaggatct
      961 gcctattggc acagaagtga aactagatgc tccttacggc gactttactc tgcataagac
     1021 cgaaacgacc cctgcagtat ttattatcgg cggtatcggt gtaacgcccg tccgcagcat
     1081 gatttcccaa gccacccatg acaaaactgc tcacaagatg accctgctcc acgctaaccg
     1141 cactccggct gacgcaccgt ttgctgctga cttcgaacga ttggctaaag aaaaccctag
     1201 ctttacttat gtgccagtag cgaccaatac atctgttacc gattggcagg gtgaacgagg
     1261 acacattgac gctgacatga ttaagcgcca tgtttccgac ctaaacgtcc ctatttacta
     1321 tctgtcgggt ccggaaggca tggtcaaagc tatgcgccaa ttgttagtta gcatgaatat
     1381 caacgaagat aacattagaa ctgaagagtt tacggggtat taaaatggcc ggcgaatttt
     1441 tatcagcaga atccgacttg aaacgagcta ttgctgatga tctctaccgc aaccctactc
     1501 taataccaga gcgaggcgta aagatagagc ttgcatcgga cgattacgct gatccagtcg
     1561 ctgatttata tcgtagagaa tatgaacgtg gcgactactt tgccagccgc tacgacgatc
     1621 cctacgaaca gatttttaac cccgaatggt tgaagcagga attccaggat cctgaacacc
     1681 tgagatttgt cttcacagac gacgagggaa aattacttgg tgcaacaggg tttttccatg
     1741 acggcgatag cgaatcgggt ccgctaatga cgagtgatgc aactcaaatt gaccctaacg
     1801 gccgtggcat gagaattatg gatcacttct ttaagcgagt cgtgccctta attgaagcgt
     1861 caggggcggg cctggcgacg gattttgttt tgacagcaga atctaaggga ctaaggcgaa
     1921 cgctgcaaac agaccttggt atggtggcaa ccggtatcca tccgcatgcc ttgcatcaca
     1981 gaaagctagg gattacccgc agtgaaattt ctgcagtcaa gaattctcgg ctcattccga
     2041 aacatgctca aatcttgcct ttctttgagc ctctctaccg tatcgttcag tctcaattgc
     2101 cccaattgcc tgagccggaa atcattccat cgcaacctca aataagatca tctatgttta
     2161 ttgactgcga ctcggaagtc gttaaaacgg ttagcggtgt tgatccacaa ggacaattag
     2221 aaccgctgga ggctggttac ttaccggttg aatttgatcc agacggcaat agatttaaag
     2281 tcgctcaata tcccagaatt tgccctgatc ttaactttgt ccttaataac gagcaggtcg
     2341 aggccaacaa gcttctagtc agatatttat acgacgtatt gtttgcaact tatcagctca
     2401 actaactatg aaaggagact actatgagcg gcgcagaaag tagcagtttc agcttcgaaa
     2461 acaaaaatgc gctggtgact ggtgccagta aaggacttgg tgaacaaatc gccttagagc
     2521 ttttgcagcg aggggtgcat gttatcggcg catccaggac tgggccacca gaatcatttc
     2581 agccatacat cgagcaagtc caagctgatt acgaactggg cgatttggcc cgtgaaggag
     2641 accgagtgat aagtgaaatc tacgacaagc atcatggcta tgagattttc gtcaataatg
     2701 ccggtgcttt ttcgccggat tactttacgc atcttgatcc ggctaaaatc cgtggcgata
     2761 ttgagcttga ccttattacg ccactactac ttcaccacag ttggtttgat ttgtataaca
     2821 agtttcatcc tagtgccaaa acgcctgagc tttcagtaaa tatttgttct atttccagct
     2881 tctatgcgtg gccgggtggt acggcttatc aggccgctaa aaccgggtta ggagcagcta
     2941 tttatggatt acggtcgatg cagcagcatc tcaatgaaca ggctacggat gaagttaagc
     3001 aacaagtcgg cccatcagct gatttggcga ccagaatcgt tgccatctac cccgacaatg
     3061 tggctaccgg ccttctctct cgtgccggtc aagaatcttt gtataaagtt caaggcgatg
     3121 ccttaccgcc cgacatagtg gtacaaactg tcatgaaagc catagaaggc caagggaagt
     3181 tcagcgaata tgatgacgtt gctatattgg tgaatccgaa tgagcctgga agcaggaagc
     3241 ggttacgggg tgtgtatctc ggattcatac ctgccgacga agagactcat cgcccgaatt
     3301 ttagcaatcg aatcttggaa aagatagccg aagaggatgt attgataaaa agagaggccg
     3361 attcatgatc aagattaaac gagtatattg ggagtacgat ccgaaggacg gcaaacggat
     3421 tcttatcgac cggctatggc ctaggggtct aagtaaagat aaagccagga tagatctatg
     3481 gttgaaggac attgctccta ccgctgaatt acgccagtgg ttcggccatg acccagggaa
     3541 gtggcctgag tttcaaaaac gctatgaaag cgaactggat gctaataaag aaagtgttga
     3601 aacgctacgg aatcagcttg aggacggacc agccacgcta gtatatggtg ctaaagacga
     3661 acaacataac gacgccgtgg tgctggcgga atatcttgaa aagtgagtaa agtatgcacg
     3721 aagccactct taccttgccc gtcaaaccgc cttttaggtt ggatctgaca gtttgggtac
     3781 ttaggcgacg ggcgaaaaat agtatcgata catgggatgg caacacttat cgccgggtat
     3841 tcactattga tggccggcct gcacttgcta aagtccacca aaaaaccggt gatttagtcg
     3901 taaaactaaa gagtagtgga acaattactt ataaacgacg aaccgagcta gaaaagctcg
     3961 tcagaagaac gctggggctg gacgttaacc ttgaaccttt ctacaaacta acagccactg
     4021 accaaaccct agagtcactt actcggcagt ttgtaggtgt gcggccccca aggtttccat
     4081 cgatattcga aacgttagtg aacgctatcg cttgccagca agtgtccctc aatgtcggta
     4141 tcttgcttat aaaccgtttg gctgatagtt tcggcaaacc aatcgtagat ggggagatga
     4201 cgctacatgc attccctgag ccacaagaca tagtggtcgt agatgaatca tcactcaaac
     4261 aacttgggtt cagctaccaa aaggtacggg ctatatccag cctggcttac gctattgcca
     4321 acaatgatgt agatctgaaa gctttgagcg ctgccagcaa tgaccaggtg atggcagttt
     4381 tacaatcgct tcgtggcatc ggccgctggt cagctgaata tgtcttgctc agggggttgg
     4441 gtaggataaa tgttttcccc ggtgacgaca ttggagggca aaacaacctg caaagattgc
     4501 ttgggttaga tatgcggcca agctatgaag agcttagaag attaacttct aaatggaatc
     4561 cctatgccgg actgatttat ttccatctgt tacttggcaa acttcgtgag aaggaattga
     4621 tatgaaccag tcgatttcgc aaaatgactc aaagctggaa ttcctggcta gacaatctga
     4681 agtggtcatt ttttgcacta cgactgcgtt tcgttttagg ccggagcgct atgagttaat
     4741 aattgataga gccaaaataa cggtgattaa gaggcttttc ctgctggcag ataatataac
     4801 aagtattggt tttggtgata tcctcgacgt caatttaagc gttggcccct tctttggcac
     4861 tttgactctg aaagcacgct acttagatca acctattcaa atctctcctt tgttacgcga
     4921 taaag
//
LOCUS       DATNGP010000134         3899 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_3056325, whole genome shotgun sequence.
ACCESSION   DATNGP010000134 DATNGP010000000
VERSION     DATNGP010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3899)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3899)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3899
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3056325"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..415
                     /gene="nrdR"
                     /locus_tag="VN778_03480"
     CDS             <1..415
                     /gene="nrdR"
                     /locus_tag="VN778_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007723580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HXR50059.1"
                     /translation="SDGQSTRRRRMCEACKYRFTTYERVERPLLVVIKNNGTRELFNR
                     AKLLAGLYRATEKTPVTNVQLERLVADIEESLYGADEEEVPTSRIGEMVMERLALLNE
                     VAYVRFASVYRRFKDITGFERELLSIKRQRVDQKS"
     gene            521..3310
                     /locus_tag="VN778_03485"
     CDS             521..3310
                     /locus_tag="VN778_03485"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF005122.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HXR50060.1"
                     /translation="MSQATSLGVGRYFTRGDTKAYDQLKWVKRDSLIMNPMTGTPVFE
                     QKGVEFPEDWSLNAINIVAQKYFTGTPGTDERESSLKQLIDRVVDTITREGLQESYFS
                     SEDEAEDFREELKYVLATQRAAFNSPVWFNIGAPNRSQQASACFILAVEDTMPSILEW
                     YRQEGMIFKGGSGSGVNVSRLRGSMEALGSSAGKASGPLSFMRGADSVAGAIKSGGKT
                     RRAAKMVILNINHPDIEEFTWCKAIEERKARVLESAGFDMTLDGKDIFSVQYQNANNS
                     VRVTDDFMQAVIDDADWQLVGVTTGKVLKTVKARDLFHQIAEAAWECADPGMQFDTTI
                     NRWHTTPKAGRINGSNPCSEYMHLDNSACNLASINLLKYLKDDNSFDVEAFKHTVEIV
                     ITAQEILVGYSEYPIEGIGKNARAYRELGLGYANLGAMLMAQGLPYDSNEGRAQAAAV
                     TALMTGHAYATSARLARIVGPFAGFDKDRVGMINVLKMHRGAVDTIDASLVSEELLSA
                     AAAAWDEAVEFGERYGVRNSQASVLAPTGTIGLMMDCDTTGIEPDLGLVKVKKLVGGG
                     TMSIVNQTVPRALAALGYTDAQIEDIVHHIDVEKTIIGAPHLKKEHEAVFACSMGDNP
                     IHYLGHVKMMSAVQPFISGAISKTVNMPEDVSIEDVEQLHIDAWKMGLKAIAIYRDNC
                     KVAQPLSMAKKEGAETTTTAVATAISAAETLDNKIVLKGAVRRKLPKVRTSRTYKFRV
                     ADLKGYFTVGEYEDGTPGELFVVVSKQGSTLAGLMDSFATAMSYGLQYGVPLKSYIKT
                     LMSRSFPPAGITDDNEIRTASSMTDYIMRRLALDYLSFDDRLELGLASLDDNPELQTT
                     LLTDDQSVGGQITAESEQPIEQVTVIAEVDVAVPASASNHDGTAPLCYNCGNQTQRAG
                     SCYVCTSCGSTTGCS"
     gene            3405..3680
                     /locus_tag="VN778_03490"
     CDS             3405..3680
                     /locus_tag="VN778_03490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50061.1"
                     /translation="MKVLILYQPRSEYARAVETFVRDFKLRHEASHLELVDVDGRDGI
                     ATATLYDVMTYPTILALRDDGSVLHSWEGAMLPVMDEVAYYTTGVNG"
BASE COUNT          967 a          964 c         1120 g          848 t
ORIGIN      
        1 gtcagatggc caatcgacac gccgccgccg catgtgtgaa gcctgcaaat atcgtttcac
       61 gacgtacgaa cgggtcgaac ggccgctact agttgtgatt aaaaataacg gtacgcgtga
      121 attgttcaat cgtgccaaat tgctggctgg actgtatcgg gcaacggaaa agactccggt
      181 aacgaatgtc cagctcgaac gtttggtggc cgatatcgaa gagagcctct acggagctga
      241 cgaagaagag gtgccaacgt cacgaattgg tgagatggtc atggaacggc ttgcactgct
      301 aaacgaagtg gcgtatgtcc ggtttgcaag tgtctaccga cgctttaaag acatcacagg
      361 atttgaacgc gagcttctga gtattaagcg gcagcgcgta gatcaaaaga gctagccaag
      421 taattggcgc acaattggtg tttccccggg ttttgagagc gcccgcctgg agacattcga
      481 aaaataaaca atcattaatc taatgcaaat gagggtagtt atgagtcaag caacaagtct
      541 aggggtggga cgatatttta cgaggggcga caccaaagcg tatgaccaac tgaagtgggt
      601 gaagcgcgat tccctcatta tgaatcccat gactggcact ccggtgttcg agcaaaaagg
      661 cgtcgaattc cccgaagact ggtcgctcaa cgccatcaat atcgtggctc agaagtattt
      721 tacagggacg ccaggtaccg acgaacgcga atcgtcgctg aagcaactga ttgaccgcgt
      781 ggtcgacact attacgcgcg aaggtctcca ggaaagctat ttcagttcag aagacgaggc
      841 ggaagatttt agggaggagc tgaaatacgt tctggccacc cagcgtgcag cttttaactc
      901 gcccgtctgg ttcaacattg gtgcgccaaa ccgttcgcag caggcaagcg cgtgctttat
      961 tcttgctgtc gaagacacta tgccatcgat tctcgaatgg taccgccagg aaggtatgat
     1021 ctttaaaggc ggctcgggtt cgggcgttaa tgtcagtcgt cttcgcggct caatggaagc
     1081 cctgggaagc agtgctggca aagcctcggg accgctcagt ttcatgcgcg gtgctgactc
     1141 agtagctgga gctattaaga gtggcggcaa gacgcggcgc gcagccaaga tggtcatttt
     1201 gaacatcaat cacccagaca ttgaagaatt cacttggtgt aaagcaattg aagagcgcaa
     1261 ggcgcgggta ctggagagcg ctggcttcga catgacgctc gacggcaaag acatcttctc
     1321 tgtgcagtac caaaatgcca ataattcagt gcgcgtaaca gatgatttca tgcaagccgt
     1381 gatcgatgat gcggactggc agctcgtggg cgtcacgacg ggcaaggtac tcaaaacggt
     1441 caaggcgcgc gacctgtttc atcaaattgc tgaggcggca tgggaatgcg ccgatccggg
     1501 tatgcaattc gatacgacca tcaataggtg gcataccacg ccgaaagcag ggcgtattaa
     1561 cgggagcaac ccctgcagcg aatacatgca cctggataac tcggcctgta acctggcgag
     1621 cattaatctg ctcaagtacc taaaagacga caacagcttt gatgtcgagg cctttaagca
     1681 cacggttgag atcgttatta ccgctcaaga gatcttggtt ggctattcag agtacccgat
     1741 cgagggcatt ggcaaaaacg cacgtgcgta ccgtgagctg ggcctcggct acgcgaactt
     1801 gggcgcgatg ctcatggcgc aaggtctgcc atacgattca aacgaaggcc gggcgcaggc
     1861 cgcggccgtc acggcgctca tgacgggtca cgcctacgcg acgagcgctc gtttggcgcg
     1921 gattgtcggg ccatttgcag gcttcgacaa agaccgagtg ggtatgatta atgtgctcaa
     1981 gatgcaccgt ggagcggtcg acacgattga cgctagcctc gtctcagagg agctgctcag
     2041 cgccgctgcg gcagcttggg atgaagctgt ggagttcggt gaacgctatg gcgtacgcaa
     2101 ctcgcaagcc agcgtgcttg cgccgacggg caccattggt ctcatgatgg actgtgacac
     2161 gaccggtatc gaaccagatc tggggctagt caaggttaag aaattagtcg gtggcggcac
     2221 gatgagtatc gtgaaccaaa cagtaccgcg tgcgctggca gccctgggct atactgacgc
     2281 tcagatagaa gatatagtac atcatattga tgttgaaaag acaataattg gcgcaccgca
     2341 cctgaaaaaa gagcatgaag ccgtcttcgc ctgctcgatg ggcgataacc caattcatta
     2401 cctaggccac gtcaaaatga tgtcggccgt ccagcccttc atctcgggcg ctatcagcaa
     2461 gacagtgaac atgccggagg atgtgagtat cgaagatgtc gagcagctac acatcgatgc
     2521 ctggaagatg ggcctcaagg ccattgctat ttaccgcgat aactgcaagg ttgcgcagcc
     2581 gttgagcatg gccaagaaag aaggagccga gactacgacc actgcggtag cgaccgctat
     2641 atcggccgct gaaacgctcg ataacaagat tgtcctgaag ggtgctgttc gccgcaagct
     2701 gccgaaagtt cgaaccagtc ggacctataa gttccgggtg gctgacctga agggttactt
     2761 taccgttggc gaatatgaag acggtactcc cggcgaacta ttcgtcgttg tctccaagca
     2821 gggctcgacg ctcgcagggc tgatggattc atttgccacg gctatgagct acggtcttca
     2881 gtatggtgtt cccctgaaga gctacatcaa aacgctcatg agccgtagct tcccgccggc
     2941 tggtattacc gatgacaatg agattcggac ggccagctcg atgactgact atatcatgcg
     3001 ccgcttagcc cttgattact tgtcatttga tgaccgcctc gagctaggat tggcgtcgct
     3061 cgatgacaat ccagagctgc agacgacact gctaactgac gaccaatcag ttggcggcca
     3121 gattacggct gaaagtgagc agccaatcga gcaggtgaca gtaatcgccg aagtcgatgt
     3181 tgccgttccg gcgtccgcct cgaatcacga cggtacggcg ccgctgtgct ataactgcgg
     3241 taaccaaacc cagcgagcag gtagttgcta cgtctgcaca agctgtggga gcacaacggg
     3301 ctgtagctag aagcaagccc tacttgagaa gaccgctgtc tgtgaataac gcagcggttt
     3361 tttcatgttt ttgtaaggta acaccgtgct acactgggga ggcaatgaaa gtgcttatcc
     3421 tgtaccagcc gcgatctgag tatgcgcgcg ccgtagagac attcgtccgc gattttaaac
     3481 tgcgccatga agccagccat cttgagcttg tagatgttga tgggcgcgac ggtattgcga
     3541 ccgccacgct ttatgatgtc atgacctatc cgactatttt ggcgctacgc gatgatggga
     3601 gtgtgctgca ctcatgggag ggtgccatgc tgccggttat ggacgaggtg gcgtactata
     3661 ctactggcgt taatggttga tacttcgctt gtataaccct ttatagtggg ttcggaagcg
     3721 caggcagccg acgcaatcta tcaacaaggc aggcgtataa cctaaggggc gatcgttgat
     3781 ataagtgctc tgcgggcgta taattaccgt tgttaaaagc gttccagctc ttagctggtg
     3841 aagcggctat caaccgctag cagggagaag aaccggtccc gaaagcctca gtagatctc
//
LOCUS       DATNGP010000135         3756 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_3641759, whole genome shotgun sequence.
ACCESSION   DATNGP010000135 DATNGP010000000
VERSION     DATNGP010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3756)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3756)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3756
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3641759"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(417..491)
                     /locus_tag="VN778_03495"
     tRNA            complement(417..491)
                     /locus_tag="VN778_03495"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(456..458),aa:Arg,seq:cct)
     gene            complement(609..1133)
                     /locus_tag="VN778_03500"
     CDS             complement(609..1133)
                     /locus_tag="VN778_03500"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018203952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006165 - obsolete nucleoside diphosphate
                     phosphorylation [Evidence IEA];
                     GO_process: GO:0006183 - GTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006228 - UTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HXR50062.1"
                     /translation="MELERTLIIFKPDAVMRGIVGEILSRFEKAGLKIVGMKMVAPDE
                     AHFRKHYEDISKMISRWGEDIFQVTLHQMTEAPVIAVVLEGIEAVSHVRKMNGTTDPK
                     DSAPGTIRGDYTHVTRAYANPRGSTLPNILHASGNPEEAAQEIALWFAPNELYEYSTA
                     YEPVVHGHIPQQKK"
     gene            complement(1311..1793)
                     /locus_tag="VN778_03505"
     CDS             complement(1311..1793)
                     /locus_tag="VN778_03505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50063.1"
                     /translation="MERLNDQSDNSQAAKNPTTMKQLGRFLLGAAEFGAGFTLAGATI
                     VTPWAPVGVLAGTLVMIDGALKIGGAVSPILKDQSRQEVEESLPQDSRLKAVAKRIGS
                     FVLGVSELAAGIAAAWVSAPTPLAVLGVPAGTAAIIDGLHRICEALPAVTLAPNAEEV
                     "
     gene            1906..>3756
                     /locus_tag="VN778_03510"
     CDS             1906..>3756
                     /locus_tag="VN778_03510"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HXR50064.1"
                     /translation="MQFSKAKTDFLEYLEIEQNRSQKTIANYDHYLTRLIDYAGDDLS
                     VQQIDSELVRKWRLWLNRLGTNTSDELQKSTSNYHLIALRSFLKFCAKRDIPAMSADK
                     IELARVVRKQVTFLNEDELERMFEQPDLSTVPGLRDRAILELLFSSGLRVSELVGLDR
                     EHVNLKRREFMVRGKGQKDRPIFISQTAADWLQRYLDKRTDNAKPLFTRYSGTKKLDY
                     GGDFKRLTARSVQRLVSRYALLAGITKHVSPHTMRHCLHAATRISLPRTIISAQELHE
                     KTSANSVKTVQWSRGFQTSRSIIQKTNHRTTELVRVMAGGYELLCTPEHRLFTIDGSG
                     IVEVEASRLKRGQYVAGVKKISQQSKHYYTADIWRLLGYICGDGHVSLRRRDILVTDK
                     DIENLEYYQSIIEKQFAKVAFIRPSKFSQSFTLVCYHMPLVKLCQKLGLDRQSIERRV
                     PAQLFASSEAHIASFLAGFYDAEGNSGGVPRYFSANKELLKDVQMLLLRLGIRAHLYD
                     RHRVVSLPHNHELLAHHMYWLHIVHKPDQEHFRLLVPTRKTFSIQPRFVGEKLPVGPI
                     LASLTKLGLENGKHLYSKWADQQSLRYPSRHTDGAIVPTKETAMRFYHRFK"
BASE COUNT          930 a          983 c          919 g          924 t
ORIGIN      
        1 atatgtggat tgccgcactc tgcaagcaat acaacttcgt gcttgctacc gccggtgccg
       61 gttttgaagc cataactggc cttgaactgc tccgctttta gaagctttat cgcgattggc
      121 caacttcgtg agagggctcg cgaagttgta aggaggagat attgaagacc ctaccctatt
      181 ttggccactt acttgccaaa atagggtagg gttgaaattt tctcggacgt ggtgccccgc
      241 cgcgaatccg tcgtcgccta tggctccttt gggatatctt cgtcgcatgc tcggtgcaag
      301 cacctgcgcg tgctcctagt tatcgaacct cagctacgcc gtacggttca aatcctggct
      361 cgagaacgag caagaaacta aaacaacctc cttcaggaga ttgttttagt ttctcgtggt
      421 gcccccggca ggattcgaac ctacagctct tccttaggac ggaatcgttt tatccattga
      481 actacggggg cttgtcgcta ttctaccggg gtccccatgc aatttacaaa aattgcatgg
      541 ggtaggtttg acggaactac tctatccatt gagctatggg acgctctggc ggctgaagca
      601 gcagattatc acttcttttg ctgaggaatg tggccgtgga caaccggctc ataggcggta
      661 ctatattcgt acaactcgtt aggtgcaaac cagagcgcta tttcctgggc tgcctcttca
      721 ggattacccg aagcatgcag gatatttggc aatgtcgagc cgcgcggatt ggcataggcg
      781 cgagtaacat gcgtgtagtc gccgcgtata gtcccgggag cggaatcctt cggatcggtc
      841 gtgccgttca tcttgcggac atggctgact gcttcaatac cctctagcac cacagcaatg
      901 accggcgctt cagtcatctg gtgcagcgtg acctggaaaa tgtcttcacc ccagcggctg
      961 atcatcttgc tgatgtcttc gtagtgcttg cgaaaatgag cttcgtctgg cgccaccatt
     1021 ttcataccga cgattttgag ccctgctttt tcaaaccggc tcagaatttc tcctacgatc
     1081 ccacgcataa cagcgtctgg cttaaaaata atcaacgtcc gttccagttc catattcttt
     1141 aagaaccttt ctgttagttg tttggtgtct attggaatct taacagatat atcggcacct
     1201 gactatgtct tcaacaatga atcaaatcgt gtccacctgt aaaaaaagaa gctcccaggc
     1261 tggagagctt cttttatatt tttacgcata cctgccgttt tggctaaaac ctacacttct
     1321 tcagcattag gcgccagagt aaccgctggt agggcctcgc atatccgatg gagcccatcg
     1381 ataatagcag ccgtaccagc aggaacgcct aagaccgcaa gcggcgttgg ggcgctcacc
     1441 catgccgcag caataccagc agcaagctca gatacgccca gaacaaagga accgatccgt
     1501 ttggctaccg cctttagtct cgagtcttgc ggcaggcttt cttcaacttc ttgccttgat
     1561 tgatccttaa gaataggcga aacggcccca cctattttca acgcgccatc gatcattaca
     1621 agagtgccag ctagcactcc aacgggagcc caaggagtta caatggttgc gcctgcaagt
     1681 gtaaagcctg cgccgaactc agctgcgcca agtaagaacc tacccaattg cttcatagtc
     1741 gttgggttct tagctgcctg gctattgtct gactgatcgt ttagcctttc cataatctta
     1801 ctccaaagtt atgtaatacg cgtaaattgt acactactgc agcaatatgt caattaatta
     1861 cgcctcagcc tgcaacctta cgacgaactg ttaaaataat atcgtatgca gtttagtaag
     1921 gctaagacgg atttcttgga gtatttggag atcgagcaga accggtcgca aaagacgata
     1981 gccaactacg accactacct gacacgcctg atcgactacg ccggtgacga tctaagcgtg
     2041 caacaaattg attcagaact ggtccgtaag tggcggctgt ggctgaaccg gctgggcacg
     2101 aacacatctg atgagctaca aaagtcgacg tcgaattacc acctaattgc gctcagaagc
     2161 ttcctcaagt tctgtgccaa gcgcgacatc ccggccatgt cagccgacaa aattgaactg
     2221 gcacgcgtgg tccgcaagca agtgacgttt ttaaacgaag atgaactgga acggatgttt
     2281 gagcaaccgg acttaagcac tgttccggga ctccgcgatc gggcaatttt ggaactgctg
     2341 tttagctcgg gcttacgtgt atctgaactg gtgggactcg accgcgagca cgtcaacctg
     2401 aaacgacgcg agtttatggt ccgcggcaaa ggccaaaaag accgaccgat ttttatcagc
     2461 cagacggcag ccgactggct gcagcgctac ctggacaagc gtactgataa cgccaagcca
     2521 ctttttaccc gctacagcgg cactaaaaag ctcgattatg ggggtgattt taagcgcctg
     2581 acggcgcggt cagttcagcg cttggtgagc cgctacgccc tgctggccgg catcacgaaa
     2641 cacgtatccc cccacacaat gcgccactgt ctgcatgcgg caacgcgcat tagcctgcct
     2701 cgaacgataa tttcagcaca agagttgcat gaaaaaacca gtgctaacag tgtaaagaca
     2761 gtgcagtggt cacgcggatt tcagaccagc agatcaatca tccaaaagac caaccatcgt
     2821 actaccgagc tagtacgggt aatggctggt ggatatgagc ttctctgcac ccctgagcat
     2881 cggctattta cgattgatgg atcagggatc gtagaggttg aagctagccg gttgaagcgt
     2941 ggacaatatg tggccggggt gaagaaaatc agccagcaaa gcaaacatta ttataccgcc
     3001 gacatctggc ggctcctggg ctacatctgt ggtgatggcc acgtaagctt gcggcggcgc
     3061 gacatattag tcaccgataa agacatcgag aatcttgaat actatcagtc gattatagaa
     3121 aagcaattcg caaaagtggc gtttatccgt ccgagtaaat tttcacaatc gtttacgctg
     3181 gtttgctacc atatgccgct cgtaaagctg tgccagaagc ttgggctcga tcggcaatct
     3241 atagaacgac gcgttccggc ccaacttttt gcctcctccg aagcgcatat tgctagcttt
     3301 ctcgccgggt tttatgatgc tgaaggtaat tctggcggcg tgccgcgcta ttttagcgcc
     3361 aataaggaac tactcaaaga tgtgcagatg ctcttgctac ggctggggat cagagcccac
     3421 ttgtatgatc gccaccgagt cgtgagcctg ccccataatc acgaactttt ggcccaccat
     3481 atgtattggc tacacattgt gcacaaaccc gatcaggagc attttcgctt gctggtgcca
     3541 acccgaaaaa cgttctctat tcaaccacgg tttgttggcg agaaactacc tgttgggcca
     3601 atacttgcat cccttaccaa gctcggtctt gagaacggta aacacctcta cagtaagtgg
     3661 gcagatcagc aaagcttacg ctatccaagc cgccacactg atggggcgat tgtgccgaca
     3721 aaggagacgg ctatgcgctt ttatcatcgt tttaaa
//
LOCUS       DATNGP010000136         2601 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_451676, whole genome shotgun sequence.
ACCESSION   DATNGP010000136 DATNGP010000000
VERSION     DATNGP010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2601
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_451676"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..364
                     /locus_tag="VN778_03515"
     CDS             <1..364
                     /locus_tag="VN778_03515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50065.1"
                     /translation="GPKYNYMINVLHVPGNIELIGYANSDAVQQAETPLKPPIQLDAL
                     LAYEDWDSVDVLHLHTVEFATAEQLKQLAGVHYSKARCIDVMTLLWYFYFVYYDLSLL
                     RRIGFFKRRAAARGMGWF"
     gene            315..956
                     /locus_tag="VN778_03520"
     CDS             315..956
                     /locus_tag="VN778_03520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50066.1"
                     /translation="MDFLKGEQRPEEWVGSDDDWLSNDSSFNDYVAYSLLPKGLFARI
                     LDKHVGENTENVGLDLAGGENGQALQDLLAAGILGRALLTTYKDRRSIATKYLVELGH
                     VEGDLIRPTTWQKIINWKHIYAPHGFALIMHRPLGALQDLNPSTYVGAANLLLNMLRP
                     GGVFFSEIPGALYGSGMTLMCRSLQEREDIADIMVGEDKLLTSALMVKTSDEQ"
     gene            1011..1355
                     /locus_tag="VN778_03525"
     CDS             1011..1355
                     /locus_tag="VN778_03525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50067.1"
                     /translation="MNSESESRKDTPRSVRRIVLQSSRTIEVVTYGNCAGQEQAAQLP
                     NPWQTEDAPIAEDFTTVAGLLRYVSYKNMSDEIDRFAAALASDNILPEDIACPATPRH
                     LERYNRLYIISE"
     gene            1368..1706
                     /locus_tag="VN778_03530"
     CDS             1368..1706
                     /locus_tag="VN778_03530"
                     /inference="COORDINATES: protein motif:HMM:NF024953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HXR50068.1"
                     /translation="MKVIFWIAYVITVAEFTASPINVLRGSEMHLKRFAEVKFPLRLA
                     RISAGIELVAVAAIIAGLWAPIARLVGGIVLAASFALLTIWAVRAKRAAGDILGLGLF
                     IASALIVALY"
     gene            1749..>2601
                     /locus_tag="VN778_03535"
     CDS             1749..>2601
                     /locus_tag="VN778_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018906692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXR50069.1"
                     /translation="MKRKKWLALLVLTGGSFMIVIDATIVNVALPSIRTSLGFTQSSL
                     AWVVNAYLLTFGGFLLLGGRLGDLYGHRRLFLGGLALFTVASLACGLSSSQAPLIVAR
                     AIQGLGGALVSAIGLSLTMDLFTEPTERAKAMGIFGFVAAGGGSVGVLLGGILTGLLN
                     WHWIFLVNVPVGAVVYFYSLRLLPAGKKAKAAESLDIAGAVTITTGLLLAVYAIVNGN
                     QVGWLTARTLGLLVASAILQASFLVIESRVKEPLMPLSLLKLRNVATANVVGVLWAAA
                     MFAFFFIS"
BASE COUNT          612 a          597 c          686 g          706 t
ORIGIN      
        1 agggccgaaa tacaactaca tgattaacgt tctgcacgtg ccgggtaaca ttgaattaat
       61 tgggtatgct aattcggatg ctgtgcaaca ggccgaaact cccctgaagc ctcctataca
      121 attagatgcg ttgctggctt acgaagactg ggattcagtc gatgtactcc acctccatac
      181 cgtagaattt gcgaccgccg agcagttaaa gcagctggct ggcgtacatt acagtaaagc
      241 tcgttgtatt gacgtaatga cacttttgtg gtatttttac tttgtttact atgacttatc
      301 cttactccgc agaattggat tttttaaaag gcgagcagcg gcccgaggaa tgggttggtt
      361 ctgacgacga ctggctaagt aatgattcta gcttcaatga ttacgtggcc tactctctac
      421 tgccgaaggg tttatttgcc aggatattag ataaacacgt gggcgaaaat acggaaaatg
      481 ttggtctgga tttagccggc ggagaaaatg gtcaagcttt gcaagattta ctggcagcag
      541 gcattctagg tcgagcgctg ttgacaacct ataaagacag acgcagcata gccacaaagt
      601 atctcgtgga gctaggacat gttgaaggtg atttaatacg gcctactacg tggcaaaaaa
      661 taatcaactg gaagcatata tacgccccgc acggatttgc gctgattatg catcggccac
      721 taggtgcact acaggactta aaccctagca catacgttgg tgcggcaaat ttgttattaa
      781 acatgttgcg cccaggcgga gtcttttttt cagaaattcc aggggctctt tatggctcag
      841 gtatgacttt gatgtgcagg tcattacaag aacgggagga tatagcggac attatggtgg
      901 gcgaagataa acttctaaca tccgctttaa tggtcaaaac cagcgatgaa caataacttg
      961 acatactata ctttattgtg tataatatgt aacaattaat tcagttcatt atgaatagcg
     1021 aatcagagtc tcgaaaggac acaccacgtt ctgttagacg cattgtgttg cagagtagta
     1081 ggacgataga ggttgtcacg tatggcaact gcgctgggca agagcaggct gcgcagctcc
     1141 ccaatccctg gcaaacagaa gacgctccta tcgccgaaga cttcaccact gtggccggcc
     1201 tactaaggta cgtatcttat aaaaatatgt ctgatgaaat agataggttt gctgccgcgc
     1261 tggcctctga caatatattg ccggaagaca ttgcctgccc ggcaaccccc cgccacctgg
     1321 agcgctacaa caggctttat atcatcagtg agtgatatca taaacacatg aaagttattt
     1381 tctggatagc ctacgtaatt acggttgccg agttcacggc ctcaccgata aacgttttgc
     1441 ggggtagcga gatgcatctt aaacgctttg cagaagttaa gttcccgcta cgcctggcgc
     1501 ggatttcggc ggggatagag ctagtggcgg tggcggccat aattgcaggg ttgtgggctc
     1561 cgattgcccg gctcgtcggg ggcatagttt tggcggcgtc atttgccctg ctgacaatct
     1621 gggctgttcg cgccaaacgc gcagcaggcg atattttggg cctaggttta tttattgcca
     1681 gcgcactaat tgtggcactc tactaaaaag caatagccaa aagctcacgg ctatgataac
     1741 ttgatgggat gaaaagaaag aaatggctgg cattgcttgt cttaaccggt ggcagtttta
     1801 tgattgtcat cgatgccacg attgtgaatg tggctctgcc gtcaattcgc actagccttg
     1861 gttttactca gtcgtcgcta gcctgggtgg tcaacgccta cttgctaacc tttggtggtt
     1921 ttctattgct gggcggccgc ctcggtgatc tctatggtca tcgccgttta tttctcggag
     1981 gtctcgccct ctttacggta gcatctttag catgcgggct ctcgagttct caagccccgc
     2041 taatcgtggc tcgagctatc caaggtttgg gcggagcttt ggtatcggcc atcggcttgt
     2101 cactcacaat ggatttattt acagagccga cagagcgtgc caaggcaatg ggcatctttg
     2161 gcttcgtggc cgctggcggc ggcagtgtcg gtgtgctgct cggtggtatc ctgaccggtt
     2221 tactgaactg gcactggatt tttctcgtca atgtgccggt tggggcggtg gtgtatttct
     2281 attcactacg gctgctgcct gcgggcaaaa aggccaaggc tgccgagagt ttagacattg
     2341 ccggcgccgt aaccattact accggtttat tgctggcagt ctatgccatt gtgaatggga
     2401 accaagtggg ctggctgaca gctcgaactc tcggcctgct cgtggcctca gcgattttac
     2461 aggccagctt tttggtcatt gaatcgcggg taaaagagcc tttgatgccg ctcagcttac
     2521 tcaaactacg caatgtcgcc acggctaatg tcgtcggcgt cttgtgggcg gctgcgatgt
     2581 tcgccttttt cttcattagc g
//
LOCUS       DATNGP010000137         2477 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_452160, whole genome shotgun sequence.
ACCESSION   DATNGP010000137 DATNGP010000000
VERSION     DATNGP010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2477
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_452160"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..387
                     /locus_tag="VN778_03540"
     CDS             <1..387
                     /locus_tag="VN778_03540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50070.1"
                     /translation="SNDDYYFFGKQRRIVDPASLFKLPLVRLTRVDYLGPEAELFYQD
                     EVRPRERTMSALGLTYTPPRELEDENIIWDEGQRCHRRRPSAEFTKLLPGSESSHVAL
                     NPEMLALVCEKVGDVIIDQYQATLTQ"
     gene            406..1665
                     /gene="nusA"
                     /locus_tag="VN778_03545"
     CDS             406..1665
                     /gene="nusA"
                     /locus_tag="VN778_03545"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HXR50071.1"
                     /translation="MDYNECYGPAKVVGFFNIERQQFGNNKKGALMDLDMKQLAVAIR
                     TIAEEKNLPEDTVKEVVEQALAAAYRRDYGDREQEIRVTMNLNTGEVDAYVTKDIVEQ
                     VENPAYEISVTEAGARRKNAKVGETMEVHEKVESFGRVAAQTAKQVILQRLREAEREI
                     VMAEYEDKIGTVINATVARVEGNIVRIDLGKAQGIMPRSEQIQGERYYPGQRLKVYLK
                     DVERGFRGPQLVVSRGNKEFIEWLFRGEVPEMESGAVEIKGISREAGVRSKIAVASNV
                     PGVDPVGTFVGGHGTRVNAVMSEVGEQEKIDIIVWAEDPIEYITNALSPTKVSKVVLD
                     DKNQKARVVVPEDQLSIAIGKNGQNVRLASKLTGFELDIEAEKEGAPAGEPEDKVKTT
                     ELPKQPKLKKKNELESSLLEAIEEHGD"
     gene            1720..>2477
                     /locus_tag="VN778_03550"
     CDS             1720..>2477
                     /locus_tag="VN778_03550"
                     /inference="COORDINATES: protein motif:HMM:NF016724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HXR50072.1"
                     /translation="MSFKGPSERFREEPEQLPAFTPDDFAVALENYIEAGTAQHTLRP
                     RQLTVFHDFLDFFQQDQGRGFVQMPTGTGKTVLFVELTKALLGARNENGDEPRILVVD
                     PLKDLVYQTLGRKKTRGFGRFAPDLKIGTFFSDSSRIEKNQIIDKQVVLTTYRSLYLM
                     HALDIIRDQTPEERDEFWRALVSEAVEKDGASFSSFSRHFANLHEQMRSREKIITGNK
                     LLDLFNVVILDEGHHAMEPRAQEVLQSISPNVPI"
BASE COUNT          676 a          610 c          657 g          534 t
ORIGIN      
        1 tcgaatgacg actactattt ttttggcaag cagcgtagga tagtcgatcc tgcttcgctc
       61 ttcaagctgc ccttggtgcg tctcacccgc gtagactacc tcggcccgga agccgagctg
      121 ttttaccagg atgaagtacg tcctcgggaa cgcacaatgt ctgccttagg gcttacgtat
      181 acgccaccgc gtgaacttga agatgagaat attatctggg atgaaggcca gcggtgtcat
      241 cgacgacggc cctccgctga attcacaaag ctgctaccgg gcagtgagag tagccacgtg
      301 gcgcttaatc cggaaatgtt agcattagtc tgcgaaaaag tcggcgacgt tattatcgat
      361 caataccagg cgacgctcac tcagtaaatt gacgtcagtc gctcgatgga ttataatgag
      421 tgctacggtc cggccaaagt ggtcggcttt tttaatatcg agaggcaaca atttggaaat
      481 aataagaaag gagctctcat ggatttagac atgaagcaac tggccgtcgc cattcggacg
      541 atcgccgaag aaaagaactt accggaagac actgtcaaag aagtggttga gcaagctctg
      601 gctgccgcct accgccgcga ctatggtgat cgcgaacaag agatccgagt aaccatgaat
      661 ctcaacaccg gcgaagtgga tgcctatgtt acgaaggaca tcgtcgaaca agtcgaaaac
      721 ccagcctatg aaatcagcgt gacagaagct ggagctcggc gtaagaacgc caaagtcggc
      781 gagactatgg aagtacacga aaaggtcgaa agcttcggcc gtgtcgccgc ccaaaccgcc
      841 aaacaagtta ttttacagcg tctgcgtgaa gccgagcgcg aaatcgttat ggctgaatac
      901 gaagacaaaa tcggcaccgt cattaatgcc accgtggctc gcgtcgaagg caatatcgtg
      961 cgcatcgatc tcggcaaagc tcaaggcatt atgcctagga gtgaacaaat ccaaggtgaa
     1021 cgttactatc ccggccagcg cctaaaagtt tatttgaaag acgtggagcg aggcttccgc
     1081 gggccgcaat tggtggtcag tcgtggcaat aaagagttta tcgagtggct cttccgcggc
     1141 gaagtgccgg aaatggaaag cggcgccgtc gagatcaaag gcatttcccg cgaagcgggc
     1201 gtgcgcagca aaatcgccgt tgccagcaat gtgcctggtg tcgatccggt cggtaccttt
     1261 gtgggtggtc atggcacgcg cgtcaatgcc gtcatgagtg aagtgggtga gcaggaaaag
     1321 attgacatca ttgtctgggc ggaagacccc atcgaatata taacgaatgc cctaagccca
     1381 accaaggtga gcaaagtagt gctagatgac aagaatcaga aagccagggt ggttgtaccc
     1441 gaagaccagc tcagcatagc cattggcaaa aacggccaaa acgtccgcct ggccagcaaa
     1501 ttgaccggct ttgagctcga catcgaagcc gaaaaagaag gcgccccagc cggtgagccc
     1561 gaagacaagg ttaaaaccac cgaactcccc aagcagccga aactcaagaa gaagaacgaa
     1621 ctcgaaagca gcttgctcga agccatcgaa gaacacggcg actaatcggc aagcaaagcc
     1681 ctctttgaca atatgtagcg atagttgtaa aatatactta tgtcatttaa ggggcctagt
     1741 gagcgttttc gcgaagaacc ggaacaattg ccggcattta cgccggacga ttttgcggtg
     1801 gctttagaaa attacattga agccggcacc gcccagcata ctttacgccc gcgtcagctc
     1861 acggtttttc atgatttcct ggattttttt caacaggatc aagggcgcgg ttttgtgcaa
     1921 atgcctacag ggacgggcaa aaccgtcctc tttgtagaat tgaccaaggc tctgctagga
     1981 gcgcgcaatg aaaacggtga tgaacccaga atactcgttg tcgatcctct taaggatctg
     2041 gtctatcaga cgctcggccg gaaaaagacc cgcggctttg ggcgttttgc gccagactta
     2101 aaaatcggca cgttcttttc tgacagctcg cgtatcgaga agaatcagat tatagataaa
     2161 caggttgtct taactacata ccgctcgctt tatctgatgc atgccctcga tataatccgc
     2221 gaccaaaccc cagaggagcg tgatgaattt tggcgggcgc tcgtaagcga ggcggtcgaa
     2281 aaggacggag cttccttctc ctcatttagc cggcattttg ccaatctcca tgaacaaatg
     2341 cggtcgcgcg agaaaatcat caccggcaat aagctattgg atctttttaa tgtcgttatc
     2401 ctggatgaag gccaccatgc tatggagccg cgtgctcaag aggtattgca gagtatctct
     2461 cctaatgtgc caattat
//
LOCUS       DATNGP010000138         3375 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_171936, whole genome shotgun sequence.
ACCESSION   DATNGP010000138 DATNGP010000000
VERSION     DATNGP010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3375
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171936"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(412..1473)
                     /gene="mltG"
                     /locus_tag="VN778_03555"
     CDS             complement(412..1473)
                     /gene="mltG"
                     /locus_tag="VN778_03555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HXR50073.1"
                     /translation="MSLAQYLAGNKKSHSHLPRRVWWLLAALLLVLIAGIFITRHIYY
                     TDLQPVSTNQKTQIFTVQTGSSVKDIASNLQKAHLIRSAWALELYVHSKELGEKVQAG
                     SYALAPNESTPEIVAIMTKGRVSTQLVTILPGRRIDQVRADLINDGFAPSDVDQALDP
                     AQYADLPVMAFKPANINTLEGLLWPDSWYKDASTPPSYIIRESLAAMGDHLTPDVQAA
                     FTAEGLTTYQGLILTSIVDQEVSKPSDQPQVAQVFLTRLKNGSTLGSDVTALYGSVAA
                     GKSPSLTYDSPYNTLIHPGLPPTPISTITNNALTAAIHPASTNWLYFVAGDNGTTYFS
                     TTLQEHNAQTQQYCHKLCQ"
     gene            complement(1470..1889)
                     /gene="ruvX"
                     /locus_tag="VN778_03560"
     CDS             complement(1470..1889)
                     /gene="ruvX"
                     /locus_tag="VN778_03560"
                     /inference="COORDINATES: protein motif:HMM:NF015607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HXR50074.1"
                     /translation="MPLTRSSILALDVGEKRIGVAVASVVARLPRPLTTLARDDRFFV
                     ALEDLVKTEDAGTIVVGLPRGLSGQETRQTAAIEEFVTELKQHFDLPIHLQDEALTSA
                     QAEAELEKSGKMYNRGDIDALAATYILEDFLLEHKEL"
     gene            complement(1873..>3375)
                     /locus_tag="VN778_03565"
     CDS             complement(1873..>3375)
                     /locus_tag="VN778_03565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50075.1"
                     /translation="LLPLAGAAGVHVAKTLTSKPSIPLAPQAFDDGEEMVAETDEPEE
                     ITTETAGDQPVGTLAGLGAAAAADDVETVELDDDETPEDVAATPKSKDFAPPTKKNKK
                     LKVPNFERFRLLLVVGALVLLLLIAGLIFALTALPKATINVKTDATNVPVNLNVNLAT
                     DTSSADPTSNTIPAKLESQVKTYTQQVPTTGQKNEGNKASGTVTITNCGPSDETIPAG
                     TGFSANGNTYISQDDVSVPQSSYSFGGQKCHNDGKATVNATAQSGGSSYNLPSGASMS
                     IASQPAYTTATSGSISGGTDNVVQVVNQNDIATAKSKISTNNDTTQKQALANQLQQEG
                     YYAITSTFSSGTPNITSSPDVGAAANSVTVTETVTYTMFGAHENDLKTLIDNAVKAQI
                     DTSKQSILSEGLDTATFGVNNSSSSSAQLTMQATAEAGPQIDVNTLKEQAAGKKPGDV
                     KSTIGNDPDVTGVEVKLSPFWVNSVPKKTSRITVNIAKPTTTTKSNATNP"
BASE COUNT          724 a          852 c          952 g          847 t
ORIGIN      
        1 ttgctcatgc gagccactgt caaggcaatg tcggcagaag aaagctgatc gagtggattg
       61 gctacggccg ccggctctga agcactgttg gcgctcaata ttttgctatc gctagtagca
      121 ttcgggctct gcagcacaaa aaccaggatg acggccagaa tgagcgcatt actcagcaat
      181 aagccaatgc gaatagcgcg ccggcgcgcc gttttgcgcc gggttttgga ctgaagatga
      241 tgatataatc tcgacgaccc ggcggtgcgt ggtctggcga gttgttctgc gattgttgaa
      301 ggggtgctgg tactaatact agcctctcac aaatattacc aagcaattat agcaaaaatg
      361 ggctgctacg caaggctttt atgtttaaat gacccctgtt ccggcgaaca attactgaca
      421 caatttgtgg caatattgct gggtttgggc attatgctct tgcagggtgg tcgaaaaata
      481 ggtggtaccg ttgtcgccgg cgacaaaata gagccagtta gtgctagccg ggtggatcgc
      541 cgctgtcaga gcattattgg tgatcgtgct gatcggcgta ggcggcaagc caggatggat
      601 caaggtgtta tacggcgaat cataggtcag actgggcgat ttaccggccg caacggaacc
      661 atacagagca gtgacatcag aaccgagcgt cgagccattc ttaagacgag tcaggaaaac
      721 ttgggctact tgcggctggt cactcggctt actgacctcc tggtcaacaa tcgaagtcag
      781 aatcagcccc tggtacgttg tgagcccctc agccgtgaag gcagcctgga cgtccggggt
      841 taaatgatcg cccatggcgg ccagagattc gcgaatgatg tacgagggcg gcgtgctggc
      901 gtctttgtac cacgaatcag gccacagcaa cccctcgagg gtatttatgt tggccggctt
      961 aaaggccata accggcaaat cagcatactg tgccgggtca agggcttgat caacatctga
     1021 cggcgcgaag ccatcattaa taaggtcagc gcgcacctga tcgatacggc ggccgggcaa
     1081 aatcgtcacc agctgcgtcg acaccctgcc cttggtcata atcgctacga tttcgggagt
     1141 actctcattt ggcgccaaag cataactacc ggcctgtact ttttcgccca gctccttgct
     1201 gtggacatac agttcgagcg cccaggccga gcgaatcaaa tgcgctttct gcaaattgct
     1261 ggctatatct ttcaccgagc taccggtctg cacggtaaag atctgggttt tttgattggt
     1321 acttaccggc tgcagatcgg tgtaataaat atgccgagta ataaatatgc cggctatcag
     1381 aacaagcaag agagcggcaa gcaaccacca tacccgccgc ggcaagtgcg agtggctttt
     1441 tttgttacca gctagatact gtgccaagct cataactcct tatgctctaa caaaaaatct
     1501 tcgaggatgt aggtggcggc cagtgcatct atatcccccc gattatacat tttaccgctt
     1561 ttttcaagct cggcctcagc ttgtgcgctg gtcagggctt catcctgcag gtgaattggc
     1621 aaatcgaaat gttgtttgag ttctgttaca aactcttcaa tggccgctgt ttgccgcgtc
     1681 tcttgcccgc tcagtccacg tggcaggccc acgacaatag ttccggcgtc ttcggttttg
     1741 acgagatctt cgagcgctac aaaaaacctg tcatcccggg cgagagttgt cagtggccgc
     1801 ggcaagcgtg cgacaaccga ggctacagcg acaccaatcc ttttttctcc cacatccaac
     1861 gccaggatgg aactacgggt tagtggcatt gctcttggtg gtggtagtcg gcttagcaat
     1921 attgaccgta atacggctgg ttttcttcgg cacactgttc acccagaaag gactgagctt
     1981 gacctcgacg ccggtcacat ccggatcatt gccgatcgtg ctcttcacat cgcccggttt
     2041 cttgccggct gcttgctctt tcagcgtgtt cacatcaatc tgcggcccgg cttcggctgt
     2101 ggcttgcatg gtcagctggg cactgctcga gctggagttg ttgacaccaa aggtcgccgt
     2161 atccaaaccc tcactcagaa tactttgttt gctggtatcg atttgagctt ttacggcatt
     2221 gtcaatcaag gttttcaggt cattctcgtg ggcaccaaac atcgtataag taactgtttc
     2281 ggtcaccgtc acactgttgg ccgctgcgcc aacatccggg ctggaggtaa tattcggagt
     2341 acccgagctg aaggtactcg taatcgcata gtagccttcc tgctggagct gattggccag
     2401 cgcctgcttc tgcgtagtgt cattattggt cgagatcttc gatttggcgg tcgcaatgtc
     2461 attctggttc acgacttgta cgacattatc cgtaccgcca gaaatcgagc cactagtggc
     2521 cgtagtatag gctggttggc tggcgatgct catggaggcg cccgaaggaa gattataaga
     2581 gctacctcca ctctgggcag tagcattgac ggtagccttg ccgtcgttgt ggcacttttg
     2641 gccgccaaaa ctatagctgc tttgaggaac tgatacgtca tcttggctaa tgtacgtatt
     2701 cccgttagcg ctaaagcctg taccagcggg gatagtttcg tccgaaggcc cgcagttggt
     2761 gatggtaacg gtgccactgg ctttgttgcc ttcgttcttt tggccggtgg tggggacttg
     2821 ttgggtgtag gttttgacct gggactcgag ttttgcgggg atggtgttgc tggtggggtc
     2881 agccgaactg gtgtcggtcg cgaggttgac gtttaagttc accgggacgt tggtggcatc
     2941 ggttttgacg ttgatggtgg ctttcgggag cgcagtaaga gcaaaaatga ggccggcgat
     3001 aaggaggaga aggacaaggg cgccgactac gagtagcagg cggaaacgtt cgaaattcgg
     3061 tactttgagc ttcttatttt ttttggtcgg gggtgcaaaa tctttagact ttggtgtggc
     3121 ggcgacatct tcgggcgttt catcgtcatc gagctccacc gtctccacgt catcagccgc
     3181 ggcagcggct ccgagaccgg ccaaggtgcc gaccggctga tcgccggcag tttcggtggt
     3241 gatttcctcg ggctcatccg tttcagctac catttcctcg ccatcatcga acgcttgagg
     3301 cgccaagggg atcgagggct tactagtcag tgttttagca acgtgaacgc cagccgcacc
     3361 agccagcggc aaaag
//
LOCUS       DATNGP010000139         5728 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_1077304, whole genome shotgun sequence.
ACCESSION   DATNGP010000139 DATNGP010000000
VERSION     DATNGP010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5728
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1077304"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..370)
                     /locus_tag="VN778_03570"
     CDS             complement(<1..370)
                     /locus_tag="VN778_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014424697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HXR50076.1"
                     /translation="MGQTINPNGKQIISVETDFCGRKLTLEVNRVGFRTSASVIARYG
                     DTVVLGTAMVSPKRLEGFDYFPLSIDYEEKFYAAGKISGSRFIKREGRPSDEAILIGR
                     IIDRPIRPLWPKGYRHEVQGV"
     gene            complement(538..804)
                     /gene="rpsO"
                     /locus_tag="VN778_03575"
     CDS             complement(538..804)
                     /gene="rpsO"
                     /locus_tag="VN778_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014094640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HXR50077.1"
                     /translation="MITSEKKAAAIKSAQVHKDDTGGASAQVAVLTERIKELTEHLKV
                     NKHDYMARRGLLQMVGKRRRLLRYIAKRDSKTYLNLIQKLGIRR"
     gene            complement(834..1124)
                     /locus_tag="VN778_03580"
     CDS             complement(834..1124)
                     /locus_tag="VN778_03580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50078.1"
                     /translation="MNPYLYDELRKLSKELGFQSVPDYIRYWVKAAVDGRTFANQALD
                     LTLPEAQAIRFIELLLAVRHVKPSSLERALDDIRQQLIYIKSTQALQKLGLR"
     gene            complement(1232..1927)
                     /gene="truB"
                     /locus_tag="VN778_03585"
     CDS             complement(1232..1927)
                     /gene="truB"
                     /locus_tag="VN778_03585"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HXR50079.1"
                     /translation="MQGLLLVDKPAGWTSFDVVNYVRKMVAAVEGKKPKNVKVGHTGT
                     LDPFATGLLVLLIGKDYTRRTSEFSKLNKTYEVTLHLGETSSTGDPEGEIMPVSSGVI
                     PTEAAIIEALQYFRGEIEQVPPAYSAIKIDGQRAYKLARAGKEVLIEPRKVTINRLEL
                     VDYIYPEARLVADVSSGTYIRTLVEDIGQALGTGAYTIGLRRTCVGQFSLDETVNINK
                     SLNSAVLITHLRR"
     gene            complement(1931..3652)
                     /locus_tag="VN778_03590"
     CDS             complement(1931..3652)
                     /locus_tag="VN778_03590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50080.1"
                     /translation="MDSAKQQLADKLKSANNVMVTVSRNPSVDQLAALLGLTLLLNKL
                     GKHAAAVFSGEVPSTLEFLQPEETIEKNTDSLRDFIIALDKSKADKLRYKVEDNVVRI
                     FITPYRTSISQEDLEFSQGDFNVDVVVALGVREQGDLDDAITMHGRILHDATVATINT
                     SADGDLGGINWHDPSASSLSELVTELGQVIDKELIDSQIATALLTGIVAETNRFSNDK
                     TTSQTMSASAALMAAGANQQLVASKLEESPRQPAPKKGNDKSSPGTDEAVPKNDNGTL
                     RIDHMEADEVEEPLQSMELPAPQEEASHEEADKKQPEPSAADTASSSTRPLMTEPPSF
                     GGSLTANAQAEGLDPVTDPLSLPKADLPQLLNHPASSQPAAPSPPILPPAPMPSAQPA
                     PTPDLTAPPPEWSPPAAPKPVTPPPAPAEPAIPEPEPAPTPPPKSSTKDKKPSKTSEP
                     TPTLTDLETSVHSPHLGNSQSIDTARDEVNKALSNVLNETPGPIQALNATPLGADLHP
                     DNPTTEGADAPSLEEQIPGLKMTPDEPSTPPAPPTPEVVNPNAPPPVPPPLPPQFTAQ
                     GNNPLQQ"
     gene            3808..4143
                     /gene="rpsT"
                     /locus_tag="VN778_03595"
     CDS             3808..4143
                     /gene="rpsT"
                     /locus_tag="VN778_03595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HXR50081.1"
                     /translation="MPIIKSAKKRVRVARKASVRNSKTKRSLKSALKLFAKSPSAKSH
                     SSAQSNLDKAVKKGVIHKNKAARLKKKSAARAKAAGVKPTKATAKKAAPAKKTAAKKP
                     AARKTTKKK"
     gene            complement(4179..4946)
                     /locus_tag="VN778_03600"
     CDS             complement(4179..4946)
                     /locus_tag="VN778_03600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50082.1"
                     /translation="MSSHPQESSKYLKDYEPQIDHLDTAALFLEHAGRKPNATEQAAL
                     AYHFETEITENIGSPEWGAATLGQRLDDMYEAAKCDLQDEAKIKQYVTNFKKARKLAS
                     KETAAQPLPAVKRKPVAKGSLPDGSAYTIYSLTKKGQRVLREEQQRKQPMPRRATKAV
                     AMLAAGVIMVPTAFKNMDVAGRARDHKPVPGNIQVIIPGLGTESTGIPEVVRKVLGSS
                     ADPGEMIGGVLWFCVYLGGFAMGGTGLAGLADVIDRR"
     gene            complement(5101..>5728)
                     /gene="holA"
                     /locus_tag="VN778_03605"
     CDS             complement(5101..>5728)
                     /gene="holA"
                     /locus_tag="VN778_03605"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HXR50083.1"
                     /translation="LSYHRFLKKATGFREFPELDQNGLARWLTDMAKERNGSLSPGDA
                     RYLVERVGANQQLLANELEKLLLYNAKITRETIDLLTDPTPQSTIFQLLEAAFVGNKR
                     RALELYAEQRALKVELPQIIAMLTWQLHILVIIKTAGDRSADDIAKEAKLNPFVIRKS
                     EGIARNLTLAQLKQLIADLLKIDVRSKRESIDPDEALQHYLLNLSEGK"
BASE COUNT         1235 a         1442 c         1522 g         1529 t
ORIGIN      
        1 cgacgccctg cacctcatgc cgatagcctt tcggccacag cggtcggatc ggacggtcaa
       61 tgatgcgccc gatcagaata gcctcgtcag atggccgtcc ctcccgtttg ataaagcgcg
      121 agccactgat cttgcctgct gcgtagaact tttcttcgta gtcaatgctc agcgggaaat
      181 aatcgaagcc ttccaagcgc ttcggactga ccatggccgt cccgagcact accgtgtcgc
      241 cataacgcgc aatgacgcta gctgaggtgc ggaagccgac gcggttgact tccaacgtca
      301 gcttgcgccc acagaagtcc gtttccacgc tgattatttg tttaccgtta gggtttatgg
      361 tttgtcccat gttttccttt cctaaagagg ttctcggcag ggttcaaata tatggcatgc
      421 cccttgtgct atttccggat gcatatatct gcagctccga cgagaaagac ccttgtagat
      481 ctattaatgt gcacaagctt gcgatcccga gcatcgggat cgttagtctg ggcacgccta
      541 gcggcgaatg ccgagtttct gaatcagatt aagatatgtt ttgctgtctc gtttagcgat
      601 gtaacgtaat aaacggcggc gcttgccaac catctgcaat aatccgcggc gagccatata
      661 atcgtgctta ttaaccttca gatgctctgt cagctctttg attcgctcag tcaatacggc
      721 cacttgggca ctagctccac cagtgtcatc cttgtgcacc tgcgcgcttt taatagccgc
      781 agcctttttc tcacttgtaa tcattgttca accattttaa cagatgacgc cctttagcgc
      841 aaccccagtt tctgcaaagc ctgtgtggat ttaatataaa ttaattgttg tcgtatatca
      901 tctaacgccc tttctagtga actgggctta acatgtctga cagctagcag aagctcgata
      961 aacctgatcg cctgagcttc tggcaatgtt aggtcaagtg cttgattcgc aaaagtgcgg
     1021 ccatccactg ccgctttgac ccagtaacgg atgtagtctg gtaccgactg aaagcccagc
     1081 tctttgctca gcttacgcag ctcatcgtat aggtaagggt tcattgaaac ttgtactttc
     1141 acgggatttc tttggtatgc cctcatactt ctccttgagt gggtagtaaa aagagtaaat
     1201 tgtgactgct cctttactct gttttacacc cttagcggcg caaatgtgta atcaaaaccg
     1261 ccgaatttaa ggatttatta atatttacag tttcatcaag cgaaaattgc ccaacgcaag
     1321 tacgtctgag gccaatagtg taggctccag tgccgagggc ttggccgatg tcttcgacta
     1381 gggtacggat ataggtaccg gaagagacgt cagcgaccag cctcgcctcc gggtagatat
     1441 agtcaaccag ctctagccgg tttatggtca ctttccgggg ctcgatgagc acttccttgc
     1501 cggcccgggc tagcttgtag gcacgttgac cgtcgatttt aatggcagag taggcaggcg
     1561 ggacttgctc gatttcgccc cggaagtatt gcaaggcctc tatgatagcg gcttcggttg
     1621 gtatcacgcc agaagaaacg ggcatgattt cgccctccgg gtcgcctgtg gagctggttt
     1681 cgcccaggtg caaggtaact tcgtaggttt tgttgagttt tgagaattcg ctggtacggc
     1741 gagtgtagtc tttaccaatg agaagcacca ggaggccagt agcaaagggg tcgagcgtgc
     1801 cggtatggcc gactttgacg tttttgggct ttttgccctc gacggccgca accattttgc
     1861 gcacgtagtt tacaacgtcg aaactggtcc agccagctgg tttatcaacg agaaggaggc
     1921 cttgcatgga ttattgctgt agcgggttgt tgccctgcgc ggtgaactgc gggggcagcg
     1981 gcggaggcac cggtggcggc gcgttcgggt taacaacctc tggcgtgggc ggagctggag
     2041 gcgtgcttgg ttcatcaggg gtcattttaa gtccggggat ttgctcttcc agggatggag
     2101 cgtcggcgcc ttcggttgta gggttatcag gatgcaggtc ggcgcccagt ggcgtggcgt
     2161 tgagcgcttg gatgggtcca ggtgtctcgt tgaggacgtt actgagggct ttatttactt
     2221 catcgcgggc cgtgtcgata ctctgtgaat tccctaagtg aggactgtgc actgaagttt
     2281 caagatcggt cagagtcggt gttggctctg aggttttact gggtttttta tccttggtag
     2341 aagatttggg aggaggcgta ggagccggtt ctggctccgg gatagccggc tcggcaggtg
     2401 caggcggcgg agtaactggt ttcggggcag ctggcggcga ccattcggga ggcggcgccg
     2461 tcaaatcagg agttggggcc ggttgagcgg acggcatggg tgccggtggc aagataggtg
     2521 gagacggcgc tgccggctga gaactagccg ggtgattcag tagctgcggt agatcggctt
     2581 tcggcaagct taaggggtcg gtgacaggat cgaggccttc ggcttgagca ttggcagtca
     2641 gcgagccacc gaagcttggt ggctccgtca taaggggccg ggttgagctc gaagcggtgt
     2701 ctgctgccga tggttcaggc tgcttcttgt cggcttcttc gtgagaggct tcttcttggg
     2761 gcgccggcaa ttccatgctt tggagaggtt cttccacttc atcagcctcc atatggtcta
     2821 tccggagtgt gccgttgtca tttttgggca cagcctcgtc ggtaccaggt gaagacttgt
     2881 cgtttccctt cttcggcgcc ggttgccttg gtgattcttc cagcttggaa gctaccagtt
     2941 gctggttggc gcctgccgcc attagcgccg cactagcgct catggtctga gatgtcgtct
     3001 tatcattgct gaaacgattg gtttcagcca cgatgcccgt gaggagtgct gtggcgattt
     3061 ggctgtcgat caattcctta tcaatgactt gtcccaactc tgtcactagc tcgcttaagc
     3121 tactggcgct agggtcatgc cagttaatgc cgccgagatc accgtcggcc gacgtattga
     3181 ttgtcgctac ggtcgcgtca tgcaaaatcc ggccgtgcat ggtgatagcg tcgtccaggt
     3241 cgccctgctc gcggacacca agcgccacca ctacatcgac attgaaatca ccttggctga
     3301 attccaggtc ttcctgactg attgatgtcc ggtagggcgt tatgaagatg cggaccacgt
     3361 tgtcttcaac cttgtagcgc agtttgtcgg ctttgctttt gtcgagagcg atgatgaaat
     3421 ctcgcaaaga atcagtattt ttctctatcg tctcttcggg ttgcaaaaac tccagagttg
     3481 agggcacttc gccgctaaag accgcggcag cgtgcttgcc caatttattg agtaacaacg
     3541 tcaagcctag taaagcggcc agctgatcta cagatggatt acggctgacc gtcaccatca
     3601 cgttattggc gcttttgagc ttgtcggcga gttgttgctt cgcgctgtcc atgtctgtct
     3661 attttattga tttgtgttag agattctgtc agatatcaga ctagcataaa cccttatgtt
     3721 tgtcgagggc gaaatgctta tttacaaatc aacgtcacct ctgtatagtt taacttcgat
     3781 acgctaaaaa cttaagcagg aacagttatg ccaattatca aatcagccaa aaaacgtgtt
     3841 cgcgtggccc gcaaggcttc cgttcgtaac tccaagacca agcgcagtct gaaaagcgcc
     3901 ttaaaactct ttgctaagag cccctcggcc aaatcgcact ccagcgccca gagcaacctc
     3961 gacaaagccg tcaaaaaagg cgtcatccac aagaacaaag ccgcccgcct gaagaaaaaa
     4021 tctgctgccc gcgccaaagc cgccggcgtc aaacccacca aggctacagc caaaaaagcc
     4081 gcaccagcca agaaaaccgc cgccaagaag cccgctgcgc gcaagactac taagaagaaa
     4141 taatcactac ttctttgatt atagttccac ttgggcaact agcgcctatc aatcacgtcg
     4201 gcgagaccag caagtccggt accccccatt gcaaagccac ctaaatagac gcagaaccac
     4261 agaactccac ctatcatctc tccaggatct gcgctactgc caaggacttt tcttactacc
     4321 tcaggtatgc cagtggactc agtccctaaa ccgggaataa tgacttgtat attcccggga
     4381 accggcttat ggtctcgggc tcgccccgcc acatccatat tcttgaaagc agtcggcacc
     4441 ataatcacgc ctgctgctag catagccacg gccttggtag ctctcctggg cataggttgc
     4501 ttacgctgct gttcttctct gagaactcgc tgacccttct tagtcaaact gtagattgtg
     4561 tacgcggaac catcgggtag gctccctttt gcgaccggtt ttctttttac cgcaggcaag
     4621 ggctgcgctg ccgtttcttt actggccagc ttacgggctt ttttgaaatt agtcacatat
     4681 tgcttaattt ttgcttcgtc ttgtagatcg catttggccg cctcatacat gtcatcgaga
     4741 cgctgaccga gtgtggcagc accccattca ggacttccaa tgttttctgt tatttcagtc
     4801 tcaaaatgat aagccagagc cgcctgttca gtagcatttg gcttgcgacc ggcatgctca
     4861 agaaatagag ccgctgtgtc taagtggtcg atttgtggct cgtagtcttt taaatacttt
     4921 gaagactcct ggggatgcga gctcatagaa aactacaata ccatatttac tataattatg
     4981 gaagatgagc tagtttcgta tatagctaga acgtagcaat tcgtactatc aatttttgtc
     5041 gtgtccccat tgtatggcca tactatgggg acactcttct tgagacgttg ttgaaaaagc
     5101 tcatttccct tcagataaat tgagcaagta gtgctgcaag gcctcgtcgg ggtctatgct
     5161 ttcgcgcttg ctgcggacgt cgatttttaa gaggtcagct atgagctgtt tcaattgggc
     5221 gagagtgagg tttcgggcta taccctcgct cttgcggata acaaaagggt tgagtttggc
     5281 ttccttggca atgtcatcgg ctgagcggtc gccagcggtt ttgataatga ccagaatatg
     5341 gagctgccaa gttaacatgg cgatgatctg aggtagttca acctttaaag cgcgctgttc
     5401 ggcgtacaat tccagggctc ggcgcttatt tccgacaaaa gcggcttcca gcagctgaaa
     5461 aatggtgctt tgcggtgtcg gatcggtgag taaatcaatc gtttcgcgag ttatttttgc
     5521 gttgtatagc agtaatttct ccagttcgtt tgccagcagc tgttggtttg cccctacccg
     5581 ctcgacgaga tatcgggcat caccgggact taacgagccg ttccgttctt tggccatatc
     5641 cgtgagccag cgcgcgaggc cgttctgatc gagctcggga aattcgcgga agccagtcgc
     5701 tttttttaaa aacctatgat aactaagt
//
LOCUS       DATNGP010000140         2188 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_1383389, whole genome shotgun sequence.
ACCESSION   DATNGP010000140 DATNGP010000000
VERSION     DATNGP010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1383389"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            240..509
                     /locus_tag="VN778_03610"
     CDS             240..509
                     /locus_tag="VN778_03610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50084.1"
                     /translation="MSLTQDDLQAIQRLIQTTVPPLIKVTVQPMIDEAVDTLAQSTAA
                     GFAEVHERIDKVEAGLGRIENKLDATIDRIDDHATRIRQLELKAV"
     gene            924..1007
                     /locus_tag="VN778_03615"
     tRNA            924..1007
                     /locus_tag="VN778_03615"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:957..959,aa:Leu,seq:tag)
     gene            1298..1582
                     /locus_tag="VN778_03620"
     CDS             1298..1582
                     /locus_tag="VN778_03620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50085.1"
                     /translation="MNKFIPITTLDSKRWTTEQAKRVWRINAAAIAYFVSHTDSFDYN
                     TQEGQDCFFAICAYFLTHPLIDEDAPGPPSSAERLRRQAELEAFTKNKCL"
     gene            1734..2081
                     /locus_tag="VN778_03625"
     CDS             1734..2081
                     /locus_tag="VN778_03625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50086.1"
                     /translation="MRRHEVRVAVTEAQAAFCALVDNASRLAKPTEGRKYHERNWEPL
                     ALPGGMTVTPQAYEQNGWPVFVLRFTAKDGNWWDVSMGGADPYTTNGYANTLGFLDVL
                     SAVNLLVHGHDEV"
BASE COUNT          576 a          522 c          577 g          513 t
ORIGIN      
        1 tagccgatga gctaatcagc cgtgcgctgc agcgcaaata cggttcgtct gagctcgaat
       61 ccctcgacga cacactcgaa caagccgccg ccgcgactgc caaacgccga cctcggtagc
      121 tactccatga tatcgctacg ctaggtttca tggggatccc gaaactatca tcgcgtaaat
      181 tcatcaaaaa ctcagaaaac ccactttaat tcgtagcatc cgcaagctat catgacagca
      241 tgtcattgac acaagacgac ctccaagcaa tccagaggct aattcaaaca acggtgccgc
      301 ctctcattaa agtaaccgtg cagccaatga ttgacgaggc agtcgatacg ctggcgcaga
      361 gtactgccgc cggattcgcc gaagtccatg aaagaatcga taaagtcgaa gctggcctgg
      421 gccggatcga aaataaactc gatgcaacta tcgaccgcat cgatgaccac gctactcgca
      481 ttcgccagct ggaactcaaa gcagtctagc ttttctctct ataatttcgt acaatacccc
      541 tcagtttcgt aagccgtttt ggcggtttag ggaaaaagag cagccgtgtt tatgcggttt
      601 ccaaaatttc aaaccaagga ggcggcttgg ccgatccgac tgtttgaaat tttgagagga
      661 agagcgtaag acgttgccgt agcttttgcc gccaggcaaa acaaggcttg tctgcaggct
      721 ctgacgggcg gatgtggtga aatggtatac atgtgtgtcc ttaggagata gcgcttaaga
      781 ggcgtggagg cgagaaccgc cagtggtggt tcgcaaggca accttctgat gaaaacttat
      841 tttcaatcat gaagttgcgg ggtcgcggaa cgtgaccaag tcctctcgaa aaatgagcct
      901 tgaacctata caatttgaac tatgcgcgag tggtggaatg gtagacacgc tagctttagg
      961 agctagtgcc gagaggcgtg gaggttcaag tcctctctcg cgcaccatag aaaaagcccg
     1021 atatcgttgg gccttttctt tgatttgcgt aaggcttgaa ccgcaaccca aagcggcgtg
     1081 gaggtgagaa ctgctggtgg cagttcgcaa gggaatcaac tcttgcaaac ttgtttgcaa
     1141 agatgttgcg acggggtcgc ggaacgtgac caagtcctct catcccacca agcaaagacg
     1201 accccaaacg ggtcgttttt gcttggtgcc cttaggtgct gtgagttatt ctttacagac
     1261 agctcggatg attttactgt actagttata gggcacgatg aacaaattta taccgataac
     1321 gacgcttgac tccaagcgct ggactactga gcaggccaaa cgggtctggc ggatcaatgc
     1381 tgctgccatt gcgtattttg tgagtcatac cgattctttt gattacaata cgcaagaggg
     1441 ccaggattgc ttcttcgcta tttgcgccta ttttctaacg catcctttaa ttgatgaaga
     1501 cgcacccggt ccaccgagta gtgccgagag actgcgccgc caggcagagt tggaggcatt
     1561 tacaaaaaac aaatgtctat aaccggggac tcggcagacg caatcagagt gatacggtgt
     1621 ataacataac gtatgcataa ggaggtgtaa tgtcggcaat agacaggcta tttgagtggg
     1681 gaccagctag cgcgactgca gctgatgcaa acgaagtata cccagccgat gatatgcggc
     1741 gtcacgaggt tcgtgtagca gtaacagaag ctcaagctgc attctgtgca ttagtggaca
     1801 acgcctctcg tctggctaaa ccaaccgaag gacggaagta tcatgaacgt aattgggagc
     1861 ctttagcgct tccgggagga atgaccgtca cgccgcaggc ttacgagcaa aatggctggc
     1921 cagtgttcgt attacgcttc accgcaaaag atggtaactg gtgggatgtg agcatgggcg
     1981 gcgcggatcc ctatacaact aacggttatg cgaacacact ggggttcctg gatgtgttat
     2041 ctgccgtgaa tctcctagtt catggtcatg atgaggtatg acctgagagt atagggcggc
     2101 acccttgaca gatagtgtat atagtgctaa tgtcaagatt atgttcaaac tagccagtct
     2161 gcatcatact catattctgc cggagcga
//
LOCUS       DATNGP010000141         2585 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_2629473, whole genome shotgun sequence.
ACCESSION   DATNGP010000141 DATNGP010000000
VERSION     DATNGP010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2585
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2629473"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1807)
                     /gene="uvrA"
                     /locus_tag="VN778_03630"
     CDS             complement(<1..1807)
                     /gene="uvrA"
                     /locus_tag="VN778_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HXR50087.1"
                     /translation="MNDEIVVKGAREHNLKNIDVTIPRGKLVVITGLSGSGKSSLAFD
                     TIYAEGQRRYVESLSAYARQFLGLMEKPDVDQIDGLSPAISIDQKSTSRNPRSTVATV
                     TEIYDYLRLLFARIGVPHCPIDGQPVVRQTTQNIVDQIAALPTGARLMILAPVIVDKK
                     GAFEHIPEQYQRAGFARVRVDGVVYALDEFPELDKAKRHRIEIVVDRLVNDDESRTRL
                     AQSVEQALEVAEGRAAVLNADTDESTSYSLMYACPDHPEVTIPELEPRNFSFNSPHGA
                     CPVCTGLGNRLEVDPELVIPNGRLTIAEGAVRPFNRINMDNWYIKKLQAVADEFGFTL
                     HQPTAEISTENMQRILYGTGDKTYKVSLGGGRSFNTTYEGVIPNLERRHKETESDFMR
                     RDIERFMQEKPCYACHGRRLKPEVLAITIGEKSIMDICELSIDDAVTHMQSLKLSDRD
                     MQIARLVLKEITARLTFLQDVGLNYLTLLRSATTLSGGEAQRIRLATQIGSGLMGVLY
                     VLDEPSIGLHQRDNDRLIRTLKHLRDLGNSVIVVEHDEDTIRTADYLLDIGPGAGIHG
                     GHVVASGTPAEVEKVAESITGQYLSGTKVIPVPKKR"
     gene            1960..>2585
                     /locus_tag="VN778_03635"
     CDS             1960..>2585
                     /locus_tag="VN778_03635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50088.1"
                     /translation="MSSYSSGETQPAYDHEPAHPISRRAILCGIAATGIAAALGGGTV
                     YHKETGWPEHFTDEIPLDPRAGDQLALIDSYDSQAAIDTSEIDNRYLDILTDKGYLRE
                     TLQQLRHTTDTPARATSAMQQSLRLVRTASEYDTRGFATVWQIDEGGIYVTAKHVVRQ
                     ALNTFTNPFTNTSVGVAASLIHPEADIALVIAPTGLPPRPAADIRLAS"
BASE COUNT          634 a          695 c          647 g          609 t
ORIGIN      
        1 ggcgcttctt tggaacagga ataactttgg tgccgcttag atattggccg gtaatactct
       61 cggccacctt ttcaacttca gcaggcgtac cactggctac tacatggcca ccatggatac
      121 cggcaccggg accgatatct agcagataat ctgcggtgcg aatcgtatct tcgtcgtgtt
      181 cgactacgat cacactattc cccagatctc gcaggtgctt aagtgtgcgg attagccgat
      241 cattatcgcg ttggtgcaaa ccaatacttg gctcgtccag cacgtacagt acgcccataa
      301 gccccgagcc aatttgggtt gccaaacgga tacgctgcgc ttccccacca ctgagtgtag
      361 tagcggaacg aagcaatgtt agataattga ggccaacgtc ttgcaggaac gttaatcggg
      421 cagtgatttc ctttaacact agccgagcaa tctgcatatc gcggtcacta agtttaagcg
      481 attgcatatg cgtaacggcg tcatcaatcg acagctcaca gatatccatg atcgactttt
      541 cgccaatagt aatggccagc acttctggct ttaaacgacg gccgtggcaa gcgtagcagg
      601 gcttctcctg cataaagcgc tcaatgtcgc ggcgcataaa atcgctttcg gtttctttgt
      661 ggcgacgctc taagtttggt atcacaccct cataggtcgt attaaaggag cggccaccgc
      721 ccagcgacac tttgtaggtt ttgtcgcccg tgccatacaa aatccgctgc atattttcgg
      781 tactgatctc ggccgttggc tgatgcagcg taaagccaaa ttcatcggcg acagcctgca
      841 gctttttgat ataccagttg tccatgttaa tgcggttaaa cgggcggaca gcaccttcag
      901 caattgtcaa acgaccattc ggtatgacaa gctcagggtc aacttccagg cggttaccta
      961 ggccggtaca aaccggacag gcgccgtgtg ggctattaaa actaaagttc cgcggctcta
     1021 gttctggaat agtaacttcg gggtgatccg ggcaggcata catgaggctg tagctagtgg
     1081 attcatcggt gtcggcattg agtaccgccg ccctgccctc tgccacttcc aaggcctgtt
     1141 caacactttg cgccaaacgg gtacgcgact cgtcatcatt gactaaacgg tccaccacaa
     1201 tttcaatgcg gtggcgcttg gccttgtcca gctcggggaa ctcgtccagc gcgtacacga
     1261 cgccgtctac acggacacgt gcaaaaccag cgcgctggta ctgctcggga atatgctcga
     1321 aggcaccttt tttgtcgaca attacggggg ctagaatcat cagccgagca cctgtcggca
     1381 gtgcggcaat ctgatccaca atattctgag tagtctgacg aaccaccggc tggccatcga
     1441 tgggacagtg tggcacccca atgcgtgcaa acaagaggcg taaataatcg tagatttcag
     1501 taacagtggc caccgtagaa cgcgggttac ggctagtgga tttctgatcg attgaaatcg
     1561 ccgggctgag gccgtcgatc tggtcgacat ccggtttttc cataagccct aggaactgcc
     1621 gagcataggc gctcagactt tcgacatagc gacgctggcc ttcggcataa atggtgtcga
     1681 acgccaagct ggatttgccg gaaccactca ggcccgtaat taccaccaac ttaccacgag
     1741 gaatcgtgac gtcgatgttc ttaaggttgt gctcccgagc tcccttaact acaatttcgt
     1801 cgttcattgt aacccgtcaa gtatagcggt tttgggccgt tttttccatg gttagttgct
     1861 gtatgccctt aagacagggg catgtagcag ggccaacacc aaagaagcct catgcaccgc
     1921 cgtcttggtc atccataaag atgttattat actgcgcata tgtcaagcta tagttccggc
     1981 gaaacacagc cagcatacga ccacgaaccg gcgcacccga taagccgtcg tgctattctt
     2041 tgtgggattg cggcaacagg gatagcggcc gcacttggag gcggcacagt ttatcataaa
     2101 gagacgggtt ggcccgaaca ctttactgat gagatacccc ttgatccgcg agcaggcgac
     2161 cagctggcgc ttattgatag ttacgactcg caagccgcaa tagatactag cgaaatagat
     2221 aatcgatacc tcgatatatt gaccgataaa ggttacttgc gagagacatt gcaacagctg
     2281 cgtcatacca cagatacgcc agctcgcgca actagcgcca tgcaacaatc cttacgctta
     2341 gtaagaacag ctagcgaata tgatacaagg ggctttgcga cagtctggca gattgacgaa
     2401 ggtggtatat acgtcacagc taaacacgtt gtcaggcagg cactcaacac cttcaccaat
     2461 ccttttacta atacatcagt gggcgtcgcg gcatcgctca tacatcccga agcagacata
     2521 gcactcgtta ttgctccaac cggcttgcct ccccgtccag ctgcagacat cagattagcc
     2581 agtag
//
LOCUS       DATNGP010000142         3164 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_483772, whole genome shotgun sequence.
ACCESSION   DATNGP010000142 DATNGP010000000
VERSION     DATNGP010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3164
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_483772"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(49..645)
                     /locus_tag="VN778_03640"
     CDS             complement(49..645)
                     /locus_tag="VN778_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019926310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HXR50089.1"
                     /translation="MPPAVPPVSLRQQKKQHTFSAIQFHALQLFHEQGYNATSVADIV
                     NKAGISEATFYRYFPTKEDVVLQDNYDPLLLASLVAQPSHLNPAQALRATFRQVLRSF
                     SSLEQQAQRERLQLIATEPKLRARMLDQLSESMRLLAEPLAKRSGRKRDDLAVLTVAG
                     AIMGACMAAMETLRTDPQADFINLLDRALAQLEPGLEL"
     gene            complement(647..2161)
                     /locus_tag="VN778_03645"
     CDS             complement(647..2161)
                     /locus_tag="VN778_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020464508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HXR50090.1"
                     /translation="MGKSGTQKWWALGAINLSVLAVALDGTILSLVLPTLAKALTASE
                     SNLEWFSAGYLLVLAAMILPAGFIGDRFGHKKLLLSSLLLFGTGSVLCAYAHTPTAFL
                     WARILMGAAGAGITVMALSTIATLFAPKERPKAVGIFSAANFIGLPLGPILGGWMLAH
                     FWWGWVFLINVPVVLLAFVVVTALMPESRATHKPKLDAFGVVSSSAGLIALIYGLITA
                     GEQGWGSVKALASMAIGAVVLAVFWVWEHRLSRQPGQEPLVDLALFRSRSYTWGAILA
                     GVMGLAMIGVLFTLPQYYQGVLGSSTMKAGIGLLPLVLGLALSAGTADLWCKLIGTKL
                     TMMLGFLFVAGSSALGAHTTISSPSSFAVIWSALLGIGVGLALASANSAALSELPKER
                     NGSGTAFLQLLQKTSGPFGATVLGSTLAAAYQSQLQLPGLPHQLVNTIHQSVFAGVAV
                     AKSLGSASLEHTVRAAFVHGMDSALVVSSGIALAGALLALLFVPRKLTVKQKSA"
     gene            2600..>3164
                     /locus_tag="VN778_03650"
     CDS             2600..>3164
                     /locus_tag="VN778_03650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50091.1"
                     /translation="MRAELGASPERVVDADAPRVYQAASIVWSNLSQLAAQQSPTAPL
                     TGTARERRNSVQDWLDAQMCLLARAMRTDGLGLYASDVVRQMPEEVRHELVRALAGEY
                     LDGPAATAAASFSMTQTSVTPEEANATMWAQSIAVLHLATRLRALDRTDAAYRLTLAG
                     LTHFHQWLRGYVFARLEGTAFMTLSREP"
BASE COUNT          769 a          839 c          905 g          651 t
ORIGIN      
        1 ccgaatatat cgatcggaaa ctattatagc ataaactctt tgaaatgtct atagttctag
       61 tcccggctca agctgagcga gtgcgcggtc caaaaggttt ataaaatctg cttgcggatc
      121 ggttcggagc gtttccatgg ctgccatgca ggctcccatg attgctccgg caactgttag
      181 cacggcaagg tcgtcacgct tgcgaccgga gcgcttggct agcggttctg caagcaggcg
      241 catgctttcg ctaagctggt caagcatacg ggcgcgcagt tttggttctg tagcaattaa
      301 ctgcaaccgc tcgcgctgtg cttgctgctc tagactcgaa aacgacctca atacttgcct
      361 gaacgtagcg cgcaaagcct gggcaggatt taaatgagat ggttgagcca caaggctagc
      421 aagtagcagc ggatcgtaat tgtcttgtag gaccacatcc tctttggtgg ggaaatagcg
      481 gtaaaatgta gcttcagaaa tacccgcctt gtttacaatg tcggcgacac tggttgcgtt
      541 gtagccttgc tcgtggaata gctgcagggc gtgaaattgg atagcgctga aggtgtgttg
      601 cttcttctgc tggcgcaaac ttaccggggg aacggcaggc ggcatgctat gctgattttt
      661 gcttaacggt aagtttacgc ggtacaaaca gtagcgctag tagtgcgcca gctagcgcaa
      721 tgccgctcga aacaaccaag gcactgtcca tgccgtgtac aaatgcagcg cggaccgtat
      781 gttcaagcga agcagaacca agcgatttgg caaccgcaac gccagcgaac acgctctggt
      841 ggattgtgtt gacaagctgg tgtggtagcc cggggagttg tagctgtgac tggtaagccg
      901 ctgccagcgt gctgccaagt actgtcgctc caaacggccc agaagtcttc tgcagtagct
      961 gcaagaacgc agtgccacta ccgttacgtt ctttggggag ctcggatagt gcggcggaat
     1021 tagcggaggc cagtgctaat cccacaccaa tgcccaggag cgccgaccag ataaccgcaa
     1081 aactactagg cgatgagata gtagtgtgag ctcccagtgc agagctgcct gctacaaaca
     1141 aaaagccaag catcatagta agcttcgtac caatcagttt gcaccataag tcggcagtgc
     1201 ctgcgctaag cgctagacca agcaccagtg gcaacaaccc aatgcccgct ttcatcgtgc
     1261 tagagccaag caccccctgg taatactgtg gcagggtaaa aagcacgcca atcatagcca
     1321 gccccatcac gccggctaag atggcgcccc aggtatatga tcgggagcgg aacagggcca
     1381 ggtcaacaag gggctcctgg ccaggctgtc tactaagacg atgttcccat acccaaaaca
     1441 cggcaagcac cacggcacca atagccatcg atgcaagagc ctttacgcta ccccatcctt
     1501 gctcgccagc ggtgataagg ccgtagatga gggcgattaa tccggcgctg gaactaacaa
     1561 cgccaaaggc atccaatttt ggtttgtgtg tggcacgcga ttccggcatc agtgcggtta
     1621 ccacaacgaa tgctagtagt acgacgggga cgtttattag gaatacccaa ccccaccaga
     1681 aatgcgccag catccagccg cccagaatcg gccctaaagg caagcctata aaattggcag
     1741 cagaaaaaat cccaactgcc ttcggtcgtt ctttgggggc aaacagggtt gcaatagtgg
     1801 agagcgccat aacggtaatg ccggcaccgg cggctcccat aagaatccgg gcccacagga
     1861 atgccgttgg tgtatgggca taggcgcata gtactgatcc ggtaccaaac agcaggagcg
     1921 aagacaacaa aagcttcttg tgtccaaagc ggtcgccgat aaatccggcg ggcaagatca
     1981 tggcggcaag cacaagtaag taacctgccg aaaaccactc cagatttgac tcggaggctg
     2041 ttagggcctt tgctagcgtc ggcagcacta ggctgagtat tgtaccgtcc agcgcgacgg
     2101 ctaaaaccga taagttaatg gcccctagcg cccaccactt ttgtgtccct gatttcccca
     2161 tactgtgccc ccgcttgagt tatttgaatg tctaaaatga tagtaactct catattttta
     2221 aaatgcaata agaataaggg ctatagaatg ataaaacagc aatgctaacg gataagagaa
     2281 gttgataatt attgacagat gtggtataat atacgagtaa tccattgtat ttgggttcgc
     2341 acaccgagac atctcatgtc tcatggaaag ataggaaatg ttcgtggcaa atgaaccgac
     2401 tcagggcgac tggcgaccga ccggcgagag gcgctggctg aggttggagg gccccgtctt
     2461 tgggcggtac aaagagctga atcgtccggg tctgaccctg accctggcgg agctgacgga
     2521 atgtcggctg ctgggcaact ggctttgcgg cgccaaccgg gagaagaaaa ccttcaccga
     2581 cagggctgtg gtccaggcgg tgcgtgccga actgggtgct agcccagagc gggttgtcga
     2641 cgccgatgcg ccgagagtgt atcaagcggc ctccattgtt tggagcaact tgtcgcaact
     2701 cgcggctcag cagtcgccga ccgccccgct taccggcacg gcccgtgagc gacgcaactc
     2761 ggtgcaggac tggctggacg ctcagatgtg cttgctcgcg agagcgatgc gcacggatgg
     2821 cctagggctg tatgcgtccg acgtcgttcg gcagatgccg gaggaagttc gacacgaact
     2881 ggtacgggcc cttgcgggtg agtatctcga cggcccggcc gctacggctg ccgctagctt
     2941 ctcgatgacg cagacaagcg tcacaccgga agaggcgaac gccaccatgt gggctcaatc
     3001 catcgcggtg cttcatctgg caactcggct gagagcactc gaccgaacgg atgcggccta
     3061 caggctgacg ctggcggggc ttacccattt ccaccagtgg cttcgaggct acgtcttcgc
     3121 tcggctcgag ggcacggcgt ttatgacgct gagcagagag cctg
//
LOCUS       DATNGP010000143         4750 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_2500115, whole genome shotgun sequence.
ACCESSION   DATNGP010000143 DATNGP010000000
VERSION     DATNGP010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4750
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2500115"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="VN778_03655"
     CDS             <1..271
                     /locus_tag="VN778_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007231901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutathione binding-like protein"
                     /protein_id="HXR50092.1"
                     /translation="EGAKNWLQILNDHWIGPKNRYLCGDELSIADYFGAAVVSIGDLV
                     GCDLSAYPDIKRWLDNMKKLKSWNQVNEVFNGFVAANKGKELVRV"
     gene            complement(409..1392)
                     /locus_tag="VN778_03660"
     CDS             complement(409..1392)
                     /locus_tag="VN778_03660"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXR50093.1"
                     /translation="MQELGRLDDAEASYRKAITQKPEYAEAHCDLGHALKRRGRLEDA
                     KASYKKAIEVKPDYALALWRLSGTAQSIPEAKFWIGECLAVDKNHEGAKLTNAALNFY
                     QGDKADFINLMQSDAKTHPLMRSFSWVFSLPKLPSLYFHKWAFFDAVVEQSVRSRPFY
                     EFGVWRGASFKYLIKTFKNGYGFDTFTGLPEDWHVGNGKYEKAGAYSSNGEIPKIEGG
                     EFVAGKFEDTLPIFFSKPRPLASVINFDADLYSSTICALDQSKPVIDKHTVLIFDELI
                     AHECWEQNEFKALNDFVFKNKFAYEVVAISFFTKQVAVKLIALTEPLAMLV"
     gene            complement(1371..2003)
                     /locus_tag="VN778_03665"
     CDS             complement(1371..2003)
                     /locus_tag="VN778_03665"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXR50094.1"
                     /translation="MRRDIQARRSIVDAKPPFGGRKKHPSQAELGGLLGYYQNGRYGD
                     AEMLGRSITQRFPRHQLSWKVLGAVLNQARKFQESLIPSQKAVDLDPKDAEARIILGS
                     TLQQLGRLEQAEASYKKAIALKPDFAAAHYCLGTMLHELGRLGDAEVSYRNSIALNPN
                     FPEAYINLGNTLRNSGRSEEAEPIYRRAIALKPDLAEAHYNLSRCKSSEG"
     gene            complement(2624..2821)
                     /locus_tag="VN778_03670"
     CDS             complement(2624..2821)
                     /locus_tag="VN778_03670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50095.1"
                     /translation="MRPFRSVLLPTLTMEVDYDPNARRWRGTHHADLLSATGRRRRSP
                     LVRRDTEQHRERVLGLPIWFD"
     gene            complement(2964..3317)
                     /locus_tag="VN778_03675"
     CDS             complement(2964..3317)
                     /locus_tag="VN778_03675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50096.1"
                     /translation="MGRKPNQICAHCNQPIATPRKRRHVGYDGWLKGHAPLIIAMHKE
                     GKGADTILGVLASKKDVMLHYGGNPHWKMALRGTIIYILARHGFAYQGAVANRTMRKS
                     PGADARLIENYDDAA"
     gene            4169..4330
                     /locus_tag="VN778_03680"
     CDS             4169..4330
                     /locus_tag="VN778_03680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50097.1"
                     /translation="MRILLGIAVVAVLVVAAWASIPTVAKQDGIASIDPTSLTVTKTN
                     LPAEQYDAF"
BASE COUNT         1070 a         1265 c         1227 g         1188 t
ORIGIN      
        1 ggaaggtgcc aaaaattggc tgcaaattct caacgaccac tggatcgggc cgaaaaaccg
       61 gtatctctgc ggcgacgaac tctccatagc tgactatttc ggcgccgctg tggtgtcgat
      121 cggcgatctg gtcggttgcg acctctctgc ctatcccgac atcaagcgct ggctcgacaa
      181 catgaagaag ctgaagtcgt ggaaccaggt caacgaggtt ttcaatggct tcgtagccgc
      241 caataagggg aaggagcttg tacgcgtgtg agcgtacggg ccttgtgttt gctggtgcgc
      301 cactgccacg tctgatgtcc gctttggatc aacagcagac atgtgcagcg cacaagccaa
      361 tgtcggcttt gtgccaatag cggacatcaa atgaaaagcg gcccgccgct aaactaacat
      421 tgcgagtggt tccgtcagcg cgattaattt tactgccact tgtttcgtaa agaacgagat
      481 agccaccacc tcataagcga acttgttttt aaaaacaaaa tcgttcaatg ctttaaattc
      541 gttttgttcc caacattcgt gagctattaa ctcatcaaag atcaacacag tgtgtttgtc
      601 tatcactggc ttcgactgat ccaaagcaca aatagtagaa gagtagaggt cggcatcaaa
      661 atttattacg gatgctagtg ggcgaggttt agagaaaaaa attggcaaag tgtcttcgaa
      721 tttacctgca acaaattctc ctccttcaat ttttggtatt tctccattgc tggaataagc
      781 gcccgccttt tcatatttac cgttcccgac atgccaatct tcaggcaggc ctgtgaacgt
      841 atcaaaacca tagccgtttt taaaagtctt aataagatat ttgaaggacg cccctctcca
      901 cacgccgaat tcgtaaaacg gtctggagcg tacactttgc tcaacaactg catcgaagaa
      961 agcccatttg tggaagtata agctgggaag tttaggtaaa ctgaataccc atgaaaatga
     1021 gcgcattaaa ggatgggttt tggcgtcaga ttgcatcaaa ttaataaaat cggctttgtc
     1081 tccctggtaa aaatttaaag cggcgtttgt taatttggcg ccctcatgat tcttatctac
     1141 cgctaaacat tcaccaatcc aaaatttggc ctccggtata ctctgcgccg taccagataa
     1201 gcgccataat gccaaagcat agtctggctt tacctcgatg gcttttttgt agctagcttt
     1261 tgcgtcttct aacctgccgc gtctcttcag cgcgtgaccc aagtcgcaat gagcttctgc
     1321 atattctggt ttctgcgtta tcgcttttct gtagcttgct tcagcatcgt ctaaccttcc
     1381 gagctcttgc agcgtgacaa gttgtaatgg gcttctgcca agtctggctt cagcgctatc
     1441 gctcttctgt aaattggttc agcctcttct gaccttccgc tgttcctcag cgtgttaccc
     1501 aagttgatat aggcttccgg aaaattagga ttgagcgcta tagaatttct gtagctgact
     1561 tcagcatcgc ccaatcttcc gagctcgtgc agcatagttc ccaagcagta atgggctgct
     1621 gcaaagtctg gttttagcgc tattgctttt ttgtagctcg cttcagcctg ttcgagcctg
     1681 ccgagttgtt gcagtgtgct acccaagata attcgggctt cggcgtcttt agggtctaga
     1741 tccactgctt tttggctagg gatcaatgac tcctgaaatt ttcgtgcttg gttgagcacc
     1801 gccccaagga ccttccagct aagttgatgg cgtggaaatc gttgagtgat tgatctcccc
     1861 aacatctcag catcgccgta tcgtccgttt tggtagtacc ctagaagacc acctaattcc
     1921 gcttgagacg gatgtttttt tcggccgcca aatggcggtt tggcatccac gatgctcctc
     1981 cgcgcttgaa tatcacggcg caaaggtgac gccttttttc aggctcagtg cataaatggt
     2041 ttataaattg tctcgtttag ataattactt gttcgtcaat tacccatgtc gagctcacca
     2101 cctcgcgggg cctccatcaa acgttcgtct gcccatgtcc gctttgggtc attagcggac
     2161 atttttcggc cagcgcgatg caagtcggct tcagtggatt cgggcacgtc tcgcaaggct
     2221 cattgtgccg aaggtggaat aaccgtcggc gccgcccagt tttttggaag aatctccaca
     2281 aacaacaagc gcagcggcat attcgatttg ttctcagcag tatgcgcgag gttccccgtt
     2341 tcaaaaaagt ctcgaccggg aggtcgcctg gctcaggcta gcacactgga agaggtcgga
     2401 gatgggcgcg tcggacagtc acccagtgtc cgctttggtc aaaagcagac ataccaagcc
     2461 gcaatcgtga tatccgccag ggacccaaag cggacatcgc gcctattgcg aacgagtatg
     2521 acgcttgcgg gaatgtttat ttgccgccgc tgcgtacggt acgaaatagg ggttctgatt
     2581 gcaccagccg cgattgcccg ccaggacagt ctgccggcat tgttcaatcg aaccatattg
     2641 gcagtcccag tacacgctct ctgtgctgtt ctgtatcacg gcgcaccaag gggcttcggc
     2701 ggcggcggcc ggttgcagac aaaagatcag cgtggtgagt accgcgccag cggcgagcgt
     2761 tcgggtcata atcgacctcc atggtcagtg tcggtaataa caccgaacgg aatggccgca
     2821 ttccaatcca gcccacggtg acgttcactt ttcattcaaa ttggtgatct ccgatccatg
     2881 tccgctttac tcccagaagc ggacattcga cctagccagc cacgtttagc gcgcgttcac
     2941 tgagcaatgc cgaacccggc aagttacgct gcatcatcgt aattttcgat gagtcgggcg
     3001 tccgcaccgg gagacttgcg catagtgcga ttggcaactg cgccctggta tgcaaacccg
     3061 tggcgagcca aaatatagat gatcgttccg cgcagggcca tcttccaatg agggttgccg
     3121 ccgtagtgga gcatcacgtc tttcttgctt gccagcacgc caagtatggt atcggcgccc
     3181 ttgccctctt tgtgcatggc gatgattaac ggtgcgtggc ctttgagcca accgtcgtag
     3241 cccacgtggc ggcgctttcg aggtgtggcg ataggctgat tgcagtgtgc gcaaatctgg
     3301 tttggtttcc ttcccatttt tttagtctcc agtacctgac cctagcccaa cggacgctta
     3361 gcgctcgctc accgcatcgg aagtgatggc ggtcacatta tgtcgctttg cagtctccag
     3421 attttggaac agatgtccgc tttgggtcac gagcagacat agcggcgtga attttggcga
     3481 tgaggtaacg gggcacttcc gccggacgtg aaatttggtg gtactatttc gtggtcgctg
     3541 cagtcgtagg cgtgcgtgat tatggcagag aataggccgt tgcccttcag cgagaggtgt
     3601 cgtgccctcg cgcaagaatg tctgacaagg gctcagttat tccggaatga gaagccgcgc
     3661 cttcagatgc tgctgtttgc ggtcgattat gcccgcaagg cgcttcaagc agagcgactc
     3721 gaagcaacaa tacaaacgct acccgatgag ggcccttcac tcattcccgg gatcgccgag
     3781 gcttttgtaa accggctcaa aaacataagt cttccgcctc cttctccacc cgagcaggcg
     3841 cgcgcgctga ggccggtgaa gaagcgacga aagacgccga ggaaacgttc gaccggacga
     3901 cagcgttccg cagacgacgc ggcacagcgg cggcttccca cctgactctg agctgtccgg
     3961 ttcgccccct ttgccgaatg tccgctttac cccaaaagtg gccattcgtc cgctcattga
     4021 aaacgcctgc tgagggccag aagcggacat atcggaacat ccgcggcaat gcgtcttgtg
     4081 tgatctccct aacattgaga ccgcacggct gggactaatg tggcaatcga gagcgaagga
     4141 ctctccgtgc aactaaagga gacctcccat gcgcatcctg ctaggcattg ctgtcgtagc
     4201 cgttctcgtc gtcgcagctt gggcgagcat tccaaccgtc gcgaaacagg acggcatcgc
     4261 gtctattgac ccgaccagct tgacggtaac caagaccaat ctgcccgcag agcaatacga
     4321 cgccttctaa ttcgcgcgag cgcaccgttg ccgtcgtaga gcataaaggc caggcattgc
     4381 ccggcctcta ggctgacgtc cgctttcggt cattagcgga ggttctcgtt tattggatca
     4441 gttccccgca gctacctagc gatgacgaat gcgtaacgcc taatgcccaa ttccagtgct
     4501 gttggtatct caaccggcag gtgtgccggg ccgctgtgca ggcacgcccc aaaggggtcg
     4561 tgtgtgaagt agctcacaca ggcatcagac agcacccgtg caaactgacg atccaggttt
     4621 ccgaggtcat ctggggtgac aattggcaac cgatgatagg cttattgtct gcgatttttg
     4681 caacaagggg cgactcatca gttccttgaa agaagtgaaa tttcgacaat ggagcaaaaa
     4741 aggctatgtc
//
LOCUS       DATNGP010000144         4012 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_2054674, whole genome shotgun sequence.
ACCESSION   DATNGP010000144 DATNGP010000000
VERSION     DATNGP010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4012
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2054674"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..408
                     /locus_tag="VN778_03685"
     CDS             <1..408
                     /locus_tag="VN778_03685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50098.1"
                     /translation="LQAYVTTHMNTNLSAGQGAVYPPIQLKYTYDRLVQAESDAAAAS
                     NTQLYTQAQNYCQTQIPTGFSGRGRVPCIEQYVQSHDTSLPTIPDALYKFDFVSPVWS
                     PDFAGWSLLFAIFTWVALIVVMVARFWLKRSVD"
     gene            complement(546..1166)
                     /locus_tag="VN778_03690"
     CDS             complement(546..1166)
                     /locus_tag="VN778_03690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50099.1"
                     /translation="MDFSNRTTQPQPVAPSAHAPVGPNAGHRKNNANEGNGWRRWGTI
                     LVAAAVIILLIALTVAVSFGNNSSDNEGTYVDTNKLQAVFLNTGQVYFGNVKKLNDKY
                     VVLTNIYYLQTSNGSSGTSSTSSNANTSVSLVKLGCELHEPYDQMVINRSQVTFWENL
                     QDGGQVAKAVKTFQQQNPNGQKCTDQSSSASTTSGNSAQNAGTSKP"
     gene            1339..1533
                     /locus_tag="VN778_03695"
     CDS             1339..1533
                     /locus_tag="VN778_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50100.1"
                     /translation="MAYQHTNSKGVTYYLNSKEVTLRGGKKQTIYYFSKDKRPEATDL
                     PAGMEVNENPRNGFLTVKRK"
     gene            1610..2368
                     /locus_tag="VN778_03700"
     CDS             1610..2368
                     /locus_tag="VN778_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXR50101.1"
                     /translation="MAETILEVKNLRKAYDDVVAVDGISFEVKKGEIFGILGPNGAGK
                     TTTLEMIETLREIDNGTATIDGINVAKHPYEIRGMIGVQPQTPGFQDKTKLTELIQMF
                     AAAYGERVDPKQFLDEVNLGEKADSFVENLSGGQKQRFSIVTALVHSPKVFFLDEPTT
                     GLDPQARRNLWNLIKEVRDRGISVILTTHYMDEAEVLCDRVAIMDQGKIIALDTPKKL
                     IRNLLDRGFKKEQHVEQANLEDVFIDMTGKGLHV"
     gene            2365..3597
                     /locus_tag="VN778_03705"
     CDS             2365..3597
                     /locus_tag="VN778_03705"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXR50102.1"
                     /translation="MRLLEIIIIAALVLRVGWRIYRRRQPTGSQAPAKPKRSLSPWQR
                     KWFTIWVFFSVNIRRLFRDRLALFFTFLFPLIFLFVFGGLSGKGGGTTFNVAIINKSQ
                     APYAKQFIAQLDKQKVFKIDKSVTSFAVANDKMGRGELDATIEFPQDFGVVRPGQANP
                     SGQAIVHYTQNSQSAATTLTSVLQAEVQGINTKLVHTTTPFTVTSKQTNERSLSSFDY
                     TFTGLLGFAILGAGIFGPINVFPELKKMGILRRLHTTPLRVWQYFLSVMLGNAVSGLM
                     SLAVMFVVAIALFHLKVVGNYLELAVFLAFSIIVILGIGLAIGGWARNERQAAPLANI
                     VVFPMMFLSGTFFPRFLMPHWLQNITTFMPLSPVIDGARLIATEGKHLTDIGPQLGIM
                     AAWLVLVYAIAFRVFRWE"
     gene            3656..>4012
                     /locus_tag="VN778_03710"
     CDS             3656..>4012
                     /locus_tag="VN778_03710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50103.1"
                     /translation="MNRQEIIRANEWAARQSWNDSGWNRGADHEVLEDVGIVSPERLP
                     AVIAQVLDAHGVAELETPTEAEEAVEAAPSASRPTTNGKRGAVDVSQLSPDEKRRRDD
                     ERQRNPAPYGTADPVVL"
BASE COUNT          954 a         1042 c         1026 g          990 t
ORIGIN      
        1 ctgcaagctt atgtcaccac gcacatgaat actaatttaa gcgcaggcca gggcgcagtc
       61 tatccgccaa tccagctaaa atacacctat gaccgattgg tgcaagccga gagtgatgcc
      121 gctgctgcta gcaataccca gctttacacc caggctcaaa attattgcca aacccaaatt
      181 ccgaccggct tttccgggcg cggccgtgta ccgtgtatcg agcaatatgt ccagagtcat
      241 gacacgtcat tgccgaccat ccccgacgcc ttgtataaat tcgattttgt ctcgcctgtc
      301 tggtcgccag acttcgccgg gtggagcttg ctctttgcca tcttcacctg ggtagccttg
      361 atagtcgtca tggttgctcg gttctggctg aagcgcagcg ttgactagta gtgttaaacc
      421 ttacatttga aagccggtgg acaatgtcgg acaaatggag cacattgtcc accaagtcgg
      481 gagtgaaagt ataataaaac gcccctccgg agagggacgt ttgaagcttg cgctacctga
      541 gccgattaag gcttagaggt gccggcgttc tgagcgctgt tgccgctggt agtgctagca
      601 ctgctgctct gatcggtaca cttctgacca tttggatttt gctgctggaa ggtcttgacg
      661 gctttagcca cctgaccacc gtcctgcagg ttttcccaga aggtgacttg gctacggttg
      721 atgaccatct ggtcatacgg ttcatgaagt tcacaaccaa gctttactag cgaaacgctc
      781 gtatttgcat tgcttgaggt tgaactggtg ccagaactac cgttggaagt ttgcaagtag
      841 tagatgtttg tcagtacgac gtacttgtca tttagtttct tgacgttgcc gaagtaaact
      901 tggccggtgt tcaagaaaac tgcctgtaac ttattagtat cgacatacgt gccttcattg
      961 tctgaagaat tgttgccgaa actgactgct accgtcaatg cgataagcag gatgataaca
     1021 gcagctgcca cgaggattgt gccccagcgc ctccatccgt taccttcatt tgcgttattt
     1081 ttccgatgtc ctgcgtttgg cccaactggc gcgtgggcac tgggggcaac aggctgcggt
     1141 tgtgtagtgc ggttagaaaa atccatacct tttttactct ccacctctta aactttttat
     1201 ggttactgac ctagtgtaaa tcatctcttg tgggagtgca actataaaaa cctcatcagg
     1261 gactaaatag cttgacaagt aaattaccac cagagtatgt taggggtaca caaactaacg
     1321 ataagaggga gagtcactat ggcttatcag catacgaact ctaaaggtgt aacctactac
     1381 ttgaattcca aggaagttac ccttcgcggt gggaaaaagc aaactatcta ctacttcagc
     1441 aaagacaagc ggcctgaagc taccgatctg ccagccggca tggaagtcaa cgagaatcct
     1501 cgcaacggct ttttgaccgt caagcgcaaa tagggcactc cctattcgta aaagagcctc
     1561 tcccagggaa aggctctttt tttgattttt tgatatagtg gaatgattaa tggctgaaac
     1621 aatcctagaa gttaaaaact tgcgaaaagc ttatgatgat gtggtggccg ttgacggcat
     1681 ttctttcgaa gtcaaaaaag gcgaaatctt cggcatcctc ggcccgaacg gtgccggcaa
     1741 aactacgacc ctggaaatga tcgaaacctt acgcgagatc gataatggta cggctaccat
     1801 agatggtatc aacgtggcca agcacccgta cgaaatccgc ggcatgatcg gggtgcagcc
     1861 gcaaacgccc ggctttcagg acaagaccaa gctgacggag ctcattcaga tgtttgctgc
     1921 ggcctacggt gaacgagtcg accccaagca gttcttggat gaagttaatc tgggagaaaa
     1981 ggcagatagc tttgttgaaa acctgtcggg tggccagaag cagcgctttt cgatcgtcac
     2041 cgctctggtg cacagcccaa aagtattttt ccttgatgag ccgacgaccg gacttgaccc
     2101 gcaagcccgc cgcaatctgt ggaatctgat aaaagaggtg cgcgaccgtg gcattagtgt
     2161 cattttgacc acgcattaca tggatgaggc cgaggtgctg tgcgaccgcg tagcgatcat
     2221 ggatcaaggt aagatcatag ctctagatac acccaaaaag cttatccgaa acctcttgga
     2281 ccgcggtttc aagaaggagc agcacgtcga gcaggccaac cttgaagatg tttttattga
     2341 catgacaggc aaggggttac acgtatgagg ttactagaga ttattatcat tgccgccctg
     2401 gttctcagag tcggttggcg tatctatcgt cggcgccagc caactggcag ccaggcgcct
     2461 gccaagccca agcggtcgct cagtccttgg cagcgcaaat ggtttacgat ctgggttttc
     2521 ttttccgtca acatccggcg cctgttccgc gaccgcttgg cgctgttctt cacgtttctg
     2581 tttccactta ttttcttatt cgtattcggt ggcctgtccg gcaagggtgg tggcacgacg
     2641 ttcaatgtgg cgattatcaa caaatcgcaa gcgccgtatg ccaagcaatt catagcccaa
     2701 ctggacaagc agaaagtatt caaaattgat aagagtgtca ccagctttgc cgtcgcaaac
     2761 gataagatgg gccgcggcga actggacgcc actattgagt ttccgcagga ctttggcgtc
     2821 gtacgtccgg gccaggctaa cccttcagga caagctattg tgcactatac gcagaacagt
     2881 cagagcgccg ctaccaccct tacgagcgta ctgcaggctg aggtgcaggg catcaatact
     2941 aaactagtgc acaccaccac cccgtttacc gtcacgagca agcagaccaa tgagcgcagc
     3001 ctcagcagtt ttgattatac ctttaccggg ctgcttggtt tcgctatttt aggtgctggt
     3061 atctttgggc cgatcaacgt ctttccggaa ctcaagaaaa tgggcattct gcgccgtctg
     3121 cacaccacgc cgctaagagt ctggcagtac ttcctgtcgg ttatgctagg caatgccgtc
     3181 tccgggctga tgtcgctagc cgtaatgttt gtcgtggcca tcgccctctt ccacctcaag
     3241 gtcgtcggca actaccttga gctcgccgtc ttcttggcgt tcagtattat cgtgatactc
     3301 ggcattggtc tggctatcgg cggctgggcc aggaacgagc gccaggctgc gccgctcgcc
     3361 aacattgtcg ttttcccgat gatgtttctc tccggcacct tcttcccgcg ctttctcatg
     3421 ccacattggc tgcaaaacat cacaactttc atgccgctgt cgccggttat cgacggtgcc
     3481 cggctgattg cgaccgaagg caaacacctg accgacatcg gaccgcagct cggcatcatg
     3541 gccgcctggc tggtcctggt atacgccatc gccttccgcg tcttccgttg ggaataaccc
     3601 ccaccttagc gtaagcacta ttgctcaaat tgcttatgta tgatataata tagctatgaa
     3661 tcgtcaagaa attataaggg ccaatgaatg ggctgctcga caaagctgga atgatagcgg
     3721 ctggaatcgg ggagctgatc atgaggtgct ggaagacgtc gggatcgttt ctccagaacg
     3781 cctgcctgcc gttatagcgc aggttcttga cgctcacgga gttgcggaat tagagactcc
     3841 caccgaggca gaggaggcag tagaggccgc tccatcagct tcaaggccga caactaacgg
     3901 taaacgcggt gccgtcgatg tatcgcagct ctctccagat gaaaaacgcc gccgtgatga
     3961 cgagcgccag cgtaatccag ctccgtatgg gacagccgat ccagtagttc tt
//
LOCUS       DATNGP010000145         3598 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_2450193, whole genome shotgun sequence.
ACCESSION   DATNGP010000145 DATNGP010000000
VERSION     DATNGP010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3598
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2450193"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..711
                     /locus_tag="VN778_03715"
     CDS             <1..711
                     /locus_tag="VN778_03715"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HXR50104.1"
                     /translation="LCGRSMCIHCHHQLSVLDLVPVVSYLWLRGKCRYCGKPIEDTPV
                     AELLVPVLFVASYVWWPQDLSAVAVTAAWVIFVAWLMCLVMFVILALYDFKWYLLPDR
                     VVFPLIGLAGIIVILRAVALGSGWDAVISAAWGVALTAGLFYAIYVISKGTWIGFGDV
                     KLAIALGLLVGGPLNALLLLFLASLFGSVSALPLLIRGKLVSSTRIPFGPFLLLATVI
                     VVLFGAGLSAWYSGLLIV"
     gene            741..1604
                     /locus_tag="VN778_03720"
     CDS             741..1604
                     /locus_tag="VN778_03720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HXR50105.1"
                     /translation="MRVGPPTEARRLRTAGFTIIETMIVLVVTGSLFVIIAATLTGRQ
                     NEAEFVHAIQSVQSQLQQAIDQVSAGFFPTVNLTCSAGASSLTFAPNPNQQGANDQCV
                     FLGSVIQFKVQNTSPERYRIYTIAGERGPGIGPTSPFQNVTPTVMGIAATGDYASYST
                     AKPLQYGLTTKWMRGDGINGAQIGAFGVLMEPGGFATSGGYNSGAQPVDLVPLPGTGL
                     SQPINLAVGAIESRLRDATLTADAPINPDNGVLICFASAGTNQSGLITIGSLGRQLVV
                     KLDIKSNTSCS"
     gene            1595..2245
                     /locus_tag="VN778_03725"
     CDS             1595..2245
                     /locus_tag="VN778_03725"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HXR50106.1"
                     /translation="MFIKRRNTSEGFTILELMIAASVFSVILLVVASGVISFTNDYYK
                     GITSSKTQDIARSVINTLTQAIQFGTTPPASLPASGNAQGFCIGNTMYSYVIGQQLSD
                     TPPDGTKHQAWHSLVADNSGCGVPNVPNSNAALLATQRELLGPHMRLSALDITSSGTL
                     YTIRVRVIYGDDDLLNRQPVPSLGAGAWSTELCAASIAGSQFCAVSDLTTTVQQRL"
     gene            2277..3329
                     /locus_tag="VN778_03730"
     CDS             2277..3329
                     /locus_tag="VN778_03730"
                     /inference="COORDINATES: protein motif:HMM:NF025699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly PilX N-terminal domain-containing
                     protein"
                     /protein_id="HXR50107.1"
                     /translation="MINSKERGVVSLIVTLVMMIVITLLVLGFAEVARNEQRSSLDDQ
                     LSAQAYYAAESGINDARAVINKAIATGGPGSVQSKGVCGNQGSYVLDPVINVARDVSY
                     TCVRVDATPKTLTKTVGYESSVLPLTSAGGTFSKLTLNWTSPSDKTAKGCYSNLASLN
                     KNPVNSAWTCNYPVLRVDLVNGMGGFSRANWSSATSTMFFVPFDASKIAVANSVAWGM
                     KGKAVPASCSSAACVAEITGLSGTDYYMRVTTLYRTGSNLTIDGNGISFKGAQATIDS
                     TGKAQDVLRRVLVAVDLTDANAYAVPGAAIVARDSVCKRFSVTNGSFQVYNDITIVGA
                     NGNTYCTVQSLGTPSP"
     gene            complement(3405..>3598)
                     /locus_tag="VN778_03735"
     CDS             complement(3405..>3598)
                     /locus_tag="VN778_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008474131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HXR50108.1"
                     /translation="NLITDGFISHNSFATDLLMNGADLRSVQAMLGHSNISTTQIYTH
                     VTDPHLKSVHEKFHSKKVT"
BASE COUNT          763 a          982 c         1002 g          851 t
ORIGIN      
        1 ctgtgcggac gatcaatgtg catccattgt caccatcagt tatcggtgct tgacctcgtt
       61 ccggtggtta gctacctctg gttgcgaggc aaatgccgct actgcggcaa accaattgaa
      121 gatacgccgg ttgccgagct actcgtgcca gtcttatttg ttgcttcgta tgtatggtgg
      181 ccgcaagacc tgagcgcagt tgcagtgacc gccgcctggg tgatttttgt agcctggctc
      241 atgtgcctgg tcatgtttgt catcttggcg ctctacgatt tcaagtggta tctactgcct
      301 gatcgcgtgg tgttccctct cattggtttg gcaggaatca tagtgattct cagagcggtt
      361 gccttgggca gcggctggga tgccgttatc tccgcagctt ggggtgttgc tctgacggct
      421 ggactatttt atgcaatcta tgtgatctca aaaggaacct ggatcggctt tggcgacgta
      481 aagctggcga tagcgctcgg tctcttggtt ggcggcccct tgaacgcgct gctactgctg
      541 tttctggcgt cactcttcgg ttcagtttcg gccttgccgc ttctcatacg gggtaagcta
      601 gtcagcagta cgcggattcc gtttggcccg ttcctgctcc tggcgacagt aattgttgtt
      661 ctgttcggcg cgggtctcag tgcctggtat agcgggctgc tgatcgtctg agacgtgccg
      721 cttatgctat actttcacct atgagggttg gcccgccgac agaggcaaga cggctgcgta
      781 cggccggttt tacgattatt gaaacgatga ttgtgctggt ggtgaccggt tccttatttg
      841 tgatcattgc cgcgaccttg accgggcgcc agaacgaagc cgagttcgtc cacgctatcc
      901 agagcgtcca atcgcagctg cagcaagcga ttgaccaggt atcggccggc tttttcccga
      961 ctgtcaatct aacctgcagt gctggagcta gctcgctgac atttgcacct aacccgaacc
     1021 agcagggcgc aaacgaccaa tgcgtctttc tcggtagtgt catccagttc aaagtgcaga
     1081 ataccagccc cgagcggtac cgcatctata ccatcgccgg cgagcgcggt ccgggtatcg
     1141 ggccaacttc gccgttccaa aatgtcacgc caaccgttat ggggattgcc gcgactggcg
     1201 actacgccag ctattcgacg gctaaaccac tgcagtacgg gcttaccacc aaatggatgc
     1261 gcggcgatgg tatcaatggc gcccagatcg gtgcgttcgg cgtgctcatg gagccggggg
     1321 gatttgctac tagcggcggc tacaattccg gcgcccaacc ggttgacctc gtaccgcttc
     1381 ccggtaccgg tctcagccaa cccattaacc tggcagtcgg tgctatcgaa tcacgactcc
     1441 gcgacgcaac gcttacggcg gatgcgccaa tcaaccctga taatggcgtg ctcatctgct
     1501 tcgcgagcgc cggcactaat cagtcggggc tgatcaccat tggcagcctc ggcaggcaat
     1561 tggtcgttaa attagacatc aagagcaaca catcatgttc ataaaacgcc gcaacaccag
     1621 cgaaggcttt accattcttg aactcatgat tgccgcgagt gtattttcag tcattttgtt
     1681 agtggttgcg agcggggtga tcagcttcac gaatgattac tataaaggta taacgagctc
     1741 taaaacgcag gatattgccc ggtcagtcat caatacgcta acgcaagcca ttcaatttgg
     1801 tacgacacct cccgcgagcc tgcccgcctc gggcaacgcg cagggtttct gtatcggcaa
     1861 tacgatgtac tcatacgtga tcggtcagca gttgtcggat acgcctcctg acggcaccaa
     1921 acaccaggct tggcatagcc tggtagctga caacagcggt tgcggcgtgc ccaacgttcc
     1981 caacagtaat gcagcacttc tggctaccca gcgcgaacta ctcggtccgc atatgcgcct
     2041 cagcgcccta gacatcactt cctccggcac gctctacacg attcgcgttc gggttatcta
     2101 cggagatgac gacctgctca accgccagcc ggtgccgagc ctgggtgccg gagcctggtc
     2161 aacggagcta tgtgccgcca gtattgctgg cagccaattc tgtgccgtgt cagaccttac
     2221 gaccaccgtt cagcaacgct tgtagaaagg gagctccata gactaccatg agcagtatga
     2281 tcaatagcaa agagcgtggg gtagtgtcac tcattgtgac gcttgtcatg atgatcgtta
     2341 ttacgctcct ggtacttggc tttgcggaag tcgcccgcaa cgaacaacgc agctcgctcg
     2401 acgaccagct gagcgctcag gcctactacg ccgccgagag cggtatcaac gacgcgcggg
     2461 cggtaatcaa caaggccatt gccacaggcg gccctggatc ggtgcagagt aagggagtgt
     2521 gtggtaatca aggtagctac gtgcttgatc cggtcattaa tgtggcgcgg gatgtctcct
     2581 atacgtgtgt tcgcgttgac gctaccccga agacgctaac caaaactgtc ggttacgagt
     2641 cgtcggtact gccgcttacc tcagcgggcg gcacctttag taagctgacg ctgaactgga
     2701 cgtcaccgag tgataagact gccaaggggt gctatagcaa tttagcaagc ctcaacaaga
     2761 atccggttaa tagtgcctgg acctgcaatt acccggtttt gcgggttgac ctagttaacg
     2821 gcatgggcgg attctctcgc gcaaattgga gtagtgcgac gtctaccatg ttctttgtgc
     2881 cgtttgatgc gtcgaagata gccgtcgcca atagtgtagc ctggggcatg aaaggcaagg
     2941 cggtaccggc aagttgcagt agcgctgcgt gcgtagccga aataacggga ctaagcggaa
     3001 ccgattacta tatgcgcgtt acgacgctct atcggactgg gtcaaaccta acaattgacg
     3061 gcaacggtat ttcattcaag ggtgcccaag caacgatcga ctcgacaggc aaagcacagg
     3121 acgtcttgcg ccgggtgctg gtggcggtcg acttgaccga tgccaatgct tacgccgtcc
     3181 caggagctgc cattgtcgct cgcgactcgg tttgcaagcg ttttagcgtc accaacggtt
     3241 cgttccaggt ctacaatgac atcaccatcg tcggcgcgaa cggcaacacc tactgcacgg
     3301 tacaatcgct cggtacgccg tctccgtaaa ggctacccca cgaaatcact gcgttaggtt
     3361 tcgtggggac cccgctaaca actaaacgca aggaaaaaaa gggattaggt cactttttta
     3421 ctgtggaact tctcgtgtac ggacttcagg tgcgggtcgg taacatgggt gtagatctgg
     3481 gtcgtgctaa tgttgctgtg gcccagcatt gcttggacgg agcgcaagtc ggcgccgttc
     3541 attaataaat ccgtcgcaaa cgaattatga gaaataaagc catctgtgat cagattct
//
LOCUS       DATNGP010000146         2149 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_3246940, whole genome shotgun sequence.
ACCESSION   DATNGP010000146 DATNGP010000000
VERSION     DATNGP010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2149
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3246940"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..270)
                     /locus_tag="VN778_03740"
     CDS             complement(<1..270)
                     /locus_tag="VN778_03740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50109.1"
                     /translation="MRRIKEIFQAFVVAVLVASSLTMGLSARSQADSTNAPSLVISQF
                     KITSSNGQFFTLYNATSATLDMSKYQLEYFNSVDLTKATSSKLIAL"
     gene            414..932
                     /locus_tag="VN778_03745"
     CDS             414..932
                     /locus_tag="VN778_03745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50110.1"
                     /translation="MLQLSANLLNKSVLSLRTGTPIATVTGAIINPNNLKIEGFYCQD
                     RYDKKELILLYQDIRDIMPKGYVVNDHDVLAEAEELVRLKDVLELNFELIGKQVETIS
                     KQKVGKVSDYAAEVETMFVQKIYVAQSILKSFTGGSLSIDRTQINEITPKRIIINDLL
                     KGTPATATAATS"
     gene            complement(922..1662)
                     /locus_tag="VN778_03750"
     CDS             complement(922..1662)
                     /locus_tag="VN778_03750"
                     /inference="COORDINATES: protein motif:HMM:NF019342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HXR50111.1"
                     /translation="MQPIIGITAGEVVNHDYPLAPIVQGQQYTYIDAIVRAGGVPIIL
                     PLVGNNEALQQLYKQCDGILFSGGNDIDPGLYDAAPSSHTKQIAPHRDEQETQLLKWA
                     LKDDKPLLGICRGMQLLNITLGGTLYQDIATELPSAEAHFIELYADRDREHHIAHQLQ
                     IKPGTKMATILGIEKLDTNAYHHQAIRNLGKDLVATAWASDGVIEGIELPGKRFVIGV
                     QSHPESLEADIEPHWQVLFSAFIEEAKK"
     gene            complement(1697..>2149)
                     /locus_tag="VN778_03755"
     CDS             complement(1697..>2149)
                     /locus_tag="VN778_03755"
                     /inference="COORDINATES: protein motif:HMM:NF014668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HXR50112.1"
                     /translation="ELSADDKLYNRTLRYVAVRPRTRWEVATYLERKHASPPLVETIL
                     NKLSNINLIDDEKFAEAFVTDRRQLRPTSRRKMIAELRKKHVAGDVIQKVLGQESGEE
                     QTALLTVIERKRRQAKYQDDLKLMQYLARQGFGYDDIKSALQASREQE"
BASE COUNT          540 a          519 c          532 g          558 t
ORIGIN      
        1 taaagcgatc agtttactgc tggtcgcttt ggtcagatcg acgctattga aatattccag
       61 ctggtacttg ctcatgtcga gcgtggcgct ggtagcattg tagagagtaa agaattgtcc
      121 gttcgagctc gtaattttga actggctaat gacgagggag ggggcattag tcgaatcagc
      181 ttgagagcga gctgatagtc ccatggttag cgagcttgct actagaaccg ccactacaaa
      241 tgcttggaat atttctttga tccgccgcat agtggaccga ttgtatgaaa cccgtcccag
      301 gcaaggcaag cagcagcatt ttcaaaatgc ctcaaatctt aaattattta agatttgctc
      361 gtatattaag caagagcaag gacagcgtct cttagaagcg ctatactaat actatgctgc
      421 aattaagcgc gaatttgctg aataaatccg ttttatcttt acgtaccggc accccgattg
      481 ccaccgttac gggcgcaatt atcaacccga ataacctcaa aatcgaaggg ttttactgcc
      541 aagaccgcta cgacaaaaag gagcttattt tgctctatca agatatccgt gacatcatgc
      601 cgaagggtta cgtcgtcaac gatcacgacg tgctggctga agccgaagaa ttagtcaggc
      661 taaaggacgt tttggagctc aacttcgagt tgatcggcaa gcaagtagaa actataagta
      721 agcagaaggt tggcaaagtc agtgactacg cagctgaggt cgaaacgatg tttgtccaga
      781 aaatctatgt tgcccagtct attttgaaaa gtttcacggg cggctcgctg agcatcgacc
      841 gcacccaaat caacgaaatt acccctaagc gcatcatcat caacgaccta ctgaagggca
      901 ctccggccac ggccacggca gctacttctt agcctcctcg ataaacgctg aaaacaagac
      961 ttgccagtgc ggctctatat cggcttccag cgactcaggg tgagactgca cgccaataac
     1021 aaagcgcttg cccggtagct caatcccttc gatcacgcca tcgctagccc aagccgtagc
     1081 aactaagtct ttacccagat tgcgtatcgc ctggtgatgg taggcattcg tatccagctt
     1141 ttcgatgccc agaatggtcg ccattttagt gccgggtttg atctgtaact gatgagcgat
     1201 gtgatgctct cgatcgcggt cggcgtatag ctctataaaa tgggcttcgg cacttggcaa
     1261 ttcagtggca atgtcttggt acagcgtgcc gccaagggtg atattgagca gctgcatgcc
     1321 gcggcagata cccagcagtg gcttgtcatc cttcagcgcc catttcagca gttgcgtttc
     1381 ttgttcatcc cggtgtgggg caatttgctt cgtatgcgat gatggtgctg catcgtatag
     1441 cccgggatca atgtcgttgc ctccggaaaa caagatgccg tcgcactgtt tataaagttg
     1501 ctggagcgcc tcgttattac caactagggg caaaataata ggcacacctc cagctcggac
     1561 aatagcatct atataggtgt attgttgccc ttggacaatc ggcgccaaag gataatcatg
     1621 attaactacc tcgccagcgg ttatcccgat aataggttgc atctatttac tgtaacatct
     1681 gggtgttgag aagggcttat tcctgctctc tggaagcttg cagggcagac ttaatatcat
     1741 cgtagccaaa gccttggcga gccagatatt gcataagttt taaatcatcc tggtattttg
     1801 cctgtcggcg cttgcgttcg atgacagtca gcagggcagt ctgctcttcg ccgctctcct
     1861 gtcccagtac cttttggata acatcgcccg ccacatgttt tttacgcagt tcggcaatca
     1921 ttttgcgacg tgaggtaggg cggagctgac gccgatcggt cacaaaagcc tcggcgaact
     1981 tctcatcatc gatcaaattg atattactta acttgttaag tatagtttca acaaggggag
     2041 gggaggcgtg tttgcgctcc agataggttg caacttccca ccgggttcga gggcgcacgg
     2101 caacatatcg taaagtgcga ttgtaaagct tgtcgtcagc ggataattc
//
LOCUS       DATNGP010000147         6768 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_1394255, whole genome shotgun sequence.
ACCESSION   DATNGP010000147 DATNGP010000000
VERSION     DATNGP010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6768
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1394255"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..439)
                     /locus_tag="VN778_03760"
     CDS             complement(<1..439)
                     /locus_tag="VN778_03760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50113.1"
                     /translation="MRKLLPLISAIVVSTGLSVGVAAADTSCSISGPTGPNSTNNCTS
                     NSSNTFNENCNNDIFLNNINDQNGSSGNATVSDNTTGGSASSGSVSNSNSTSNQVSAS
                     CASQAQTSTAITPAVGGRGGGAGAAATPAAVVAVTSPGRGAAIQ"
     gene            614..1339
                     /locus_tag="VN778_03765"
     CDS             614..1339
                     /locus_tag="VN778_03765"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXR50114.1"
                     /translation="MKLLLIEDDRHIVQALKTALTINYKLTHAYDGVTGLALAANDPY
                     DVIILDVMLPDMGGADVCQNLRTGGYHGPILILSGETKIMTKIVLLDIGADDYLTKPF
                     SLGELKARLRVLTRRTHLSASRPGVWVVGDLTLDHATHRVERSGEVIQLRRKEFALLE
                     CLVQRAGTVVTRETLGAHAWHKNEDPWTNTIEVHIKNLRDKVDRPFSIPIIQTVHGVG
                     YKLQAAANNTHKDAEQSKKLLIA"
     gene            1344..2723
                     /locus_tag="VN778_03770"
     CDS             1344..2723
                     /locus_tag="VN778_03770"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HXR50115.1"
                     /translation="MQAVLRYSYDLFLAPRSLSDDMRRREFILNVLLTSLALVTCLLF
                     VFSVLHALDLQIDQQEGLATLYALGLSVVFGLWRLSRNSKQQLASLGLLLLLYVAATR
                     LTIHYSFELPISELMYAVLIVFAGILLKTHDALVTAGIVVATLLGVSYVQTAHYMTPR
                     LDWLNQDFGLGDAIGYMAMLCLIALVSWLANRDIHRSLARAQDSEAALVKERDELEVK
                     VAERTQELEQTQLLRTIELERFANLGRVSAHLLHELVNPLTAASLHLNQFMDQQSPLI
                     AETRQSLWQMERYIKAARRQLDMKSQLNMFSVRAELNLVLRIMRPLARRNNITLTAKV
                     NTAPALFGDPVKFNQLATNLLANSLDACAEARHGSTQGQVQIIVNANGQWLKIQVIDN
                     GEGIKESQLPFIFEHFYSTKVNGRGTGLGLPLVKKIAEEDFQGNVTVTSMPGEGTVFT
                     VRLLLPEKT"
     gene            complement(2817..3392)
                     /locus_tag="VN778_03775"
     CDS             complement(2817..3392)
                     /locus_tag="VN778_03775"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016477113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HXR50116.1"
                     /translation="MATELATFLNGPAWEVAPRLLGCELVREVDGRILRGRIVEAEAY
                     DQTDVASHSYRGETPRTKVMFGPPGHLYVYFTYGMHYCCNVVTGPAGHGSAVLIRALE
                     PLSGEEYMSQFRHGLSSEALTNGPAKLCQALQIDKQWNGHDLRLAPLRLVIREALPQT
                     QVIQSTRIGISHAKDVPWRFYIKGNSYVSKL"
     gene            complement(3382..4218)
                     /locus_tag="VN778_03780"
     CDS             complement(3382..4218)
                     /locus_tag="VN778_03780"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXR50117.1"
                     /translation="MVLRFFDRFWHRALGRPYYLARTIDEGEGPPVVLLHGIGRTGEV
                     WRHVVEQLQTKSYRVVAFDLLGFGASPKPEWLRYTVDDHVTAVIASLERLKLREPVIL
                     VGHSMGCLVAVRMAHLRPDLVKHLVLYEMPLYEGLPQKRLYRVRLNLYFRFYNRLIRL
                     QPTFSPENARLAERLAPRIVGFEVTEDTWQPFVKSLENTIMSQTAAEDIKQLAMPMDV
                     IYGMFDMLVIRGKPTTIFGVDNANITAHTIRARHMISPKASRFLVSRIVAASQEEVMN
                     GN"
     gene            4282..5286
                     /locus_tag="VN778_03785"
     CDS             4282..5286
                     /locus_tag="VN778_03785"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HXR50118.1"
                     /translation="MHSGPGSTALIIIIVIILIIFSAVCSGLNIAVMSLDLADLRRKA
                     KLGNRGAKRVLPLRKQAHLTLASILLSNVAAVSATSLVLDQRLNGWLAGLLGTLLIVI
                     FGEVMPQALFSKNPLAWSSRFAPVLQAMIVVTYIVSKPLQVLLDHLFPHERAKLQTRH
                     ELGLMINEHLSDDSSELDEDEVEIMRGALQLSEKRVRDIMTDIRHTYWLGLDTALDGQ
                     KIDEIKAVGYSRIPIFDHALKECYGLLLMKDLVDIDFDERTLLVRDLLLRPTKLVGSM
                     TALDTMFRKFIAASTHLIPIEKDEEIIGIVTIEDLIEEIIGHEIQDETDRQKDSTTRI
                     "
     gene            5387..5845
                     /locus_tag="VN778_03790"
     CDS             5387..5845
                     /locus_tag="VN778_03790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50119.1"
                     /translation="MVKQVADAHHVWNRQLIIIGLPLIITLAFGFGLAQMQLPKTKAQ
                     QITPSASSPQSLPPAVESSKSLPMLPFAPSKKLPSVTSSSSIDSNSSQTATPQPAAQT
                     TTNLQAAATGSQPTSPSNSQQDLPQVLDVLHSIRNINTQPLNISVTQNLL"
     gene            5885..6373
                     /locus_tag="VN778_03795"
     CDS             5885..6373
                     /locus_tag="VN778_03795"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HXR50120.1"
                     /translation="MTALESDLEVLERAMRMFFQTMKRPQHWARVSARSGLAIDRPSS
                     AILHILMHSKSHACRVQDVAHLLGVEAPSVTRKTQELELSGLIRRVPDSRDKRAVDLR
                     ITPRGRTIAKRLWQAQREVMYQTLANWDESERRTFVKLFERFSQDYVSSVEAEHLNKR
                     KD"
     gene            6379..>6768
                     /locus_tag="VN778_03800"
     CDS             6379..>6768
                     /locus_tag="VN778_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004543565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXR50121.1"
                     /translation="MSELAQTAAPTEHHEGERSHAEIMVIMTALMLALLLAALDQTIV
                     STALPRIANDLHGLSKYSWVATAYLLTSAVSTPLYGKISDMFGRKKIFQSAIVIFLIG
                     SALCGLSQNMNQLIVFRGLQGLGAGGLM"
BASE COUNT         1701 a         1743 c         1638 g         1686 t
ORIGIN      
        1 cttgtatggc tgctcctctg ccaggagatg tgacagcgac tacagcagcc ggagtggccg
       61 cagcgcctgc accaccacct cgtccgccaa cggccggcgt aatagcagta ctagtctgag
      121 cttgcgaggc gcagctagcg ctcacctggt tgctcgtaga attgctgtta ctcacgctcc
      181 ccgatgaagc actaccacca gtggtattgt cgctgaccgt agcgttcccg gagctgccat
      241 tttgatcatt gatattgttt aggaaaatgt cgttgttgca gttttcgttg aatgtatttg
      301 agctgtttga ggtacagtta tttgttgaat tcgggcctgt tggtccgcta atcgaacagc
      361 tggtgtccgc cgcagccacg ccaacgctca ggccggtgct gacaacaatt gccgatatta
      421 atggtaataa tttcctcact tcgctcctcc ttctccttaa gttggtgctt tagttgtata
      481 ccttttaacg ccgcccgtgt gtaataaata tgacagtcta caaaaaacca cgtctcccat
      541 tagtttttac cccaaaattt aataattatt taataactcc ctgccaaaat actcccagaa
      601 aggaacttcc gggatgaaat tattgttaat tgaagatgat cggcatattg tgcaagcgct
      661 taaaacagca ttgacgataa attataagct cacccatgcg tacgatggtg tcactggtct
      721 agccctggcg gcgaatgatc cgtacgatgt aattatttta gacgtgatgt tacctgacat
      781 gggcggggct gacgtctgcc agaatctcag aacaggcggc taccatgggc cgatcttgat
      841 ccttagcggc gaaaccaaaa ttatgaccaa aatagtgttg ctagacattg gcgcagatga
      901 ttacctgact aagccattca gtcttgggga gttgaaggct cgcttgcgtg tacttacacg
      961 ccgaacccat ttgtctgcgt cccgtccagg tgtgtgggta gttggtgatt tgacccttga
     1021 tcacgctacc caccgtgtgg agcgcagcgg cgaagtcatc cagcttcgcc gaaaagaatt
     1081 tgcgctactc gaatgcctgg tgcagcgtgc cgggactgtg gtgacgcgcg aaacattagg
     1141 cgctcatgcc tggcacaaga atgaagatcc ttggactaac acaatcgagg tgcacataaa
     1201 aaatcttcgc gataaagtcg accgtccctt ttcaatacca atcatccaaa ccgtacacgg
     1261 agtcggctat aagctccagg cagcagcaaa caatacacat aaggacgcag agcagtctaa
     1321 aaagctgtta atagcctgaa ttaatgcaag ctgtcttacg ttatagttac gatctttttc
     1381 tggcaccacg aagcctgtca gatgatatgc gacggcgtga attcatcctg aatgtactcc
     1441 ttacaagttt ggctcttgtt acttgcttat tgtttgtatt ttctgtcctg cacgccctgg
     1501 atttacaaat tgaccagcag gaaggtttgg ctactttata cgctctcggt ttaagtgtag
     1561 tcttcgggtt atggcgattg tcgagaaata gcaaacagca acttgccagc ctggggttac
     1621 ttttattgtt atatgtggcg gcaaccagac tgactatcca ttatagtttt gaattaccga
     1681 ttagcgagtt gatgtatgca gtgctaatag tatttgcggg cattttatta aaaacacatg
     1741 acgcgttagt taccgctggc atagtcgttg ctactttgct cggcgttagc tatgtccaaa
     1801 cggctcacta tatgactcct cgcctcgatt ggctgaatca agattttggt ttgggtgatg
     1861 ccataggcta tatggctatg ctctgcctca tcgccttggt gtcgtggctc gccaatcgag
     1921 atatacatcg ttccctcgca cgagcgcaag actcggaagc agctctggtt aaagaaagag
     1981 atgagctaga agttaaagta gctgaacgaa cccaggaact tgagcagact cagttactga
     2041 gaactataga gctagaacgc tttgcgaacc ttgggcgggt cagcgctcat ttacttcatg
     2101 aacttgtaaa ccctctgaca gcagcctcac tccatctgaa ccagttcatg gatcaacaat
     2161 caccactcat agctgagact cggcaaagtt tgtggcaaat ggaacgatat atcaaggcag
     2221 ctcggcggca acttgatatg aagtctcaac tcaacatgtt ttcggtacgc gctgaattga
     2281 atttggtcct acgtattatg cgtccgctgg cgcgtcgcaa caacataacc ctgacagcta
     2341 aagttaatac tgctcccgca ctttttggag atcctgtgaa attcaatcaa ctggctacta
     2401 acctccttgc caactcgcta gatgcttgcg cagaggcacg acatggctca actcaagggc
     2461 aagtacaaat tattgtcaac gccaacggac aatggctgaa aatacaagtc attgataatg
     2521 gcgaagggat aaaagagtcg cagctgcctt ttattttcga gcatttttac agtacaaaag
     2581 taaatggtcg aggcactggg ctcgggttac cattggttaa gaaaatagcc gaagaagatt
     2641 ttcaaggtaa cgtaactgtt actagtatgc ctggtgaggg cacggtattt acggtgaggc
     2701 ttttattacc cgagaaaaca tagacccggg agaaaaacgc ccgggtcgtt aggaagcctc
     2761 acacttccta gtattcagct taggtcgcct gagagtaaag tgcaatgtga ttttaatcac
     2821 agtttagaga cgtagctgtt gcctttaata taaaagcgcc agggtacatc cttggcatgg
     2881 gagatgccga ttcgggtgct ttggatcacc tgagtttggg gcaatgcttc tcggattact
     2941 aatcttagtg gcgccaaccg cagatcgtgt ccattccatt gcttatcaat ctgtaaagcc
     3001 tggcacaatt ttgccgggcc atttgttaac gcttcgctcg agagcccatg tcggaattgg
     3061 ctcatatatt cctcaccgct taagggctcc agcgcccgga taagtacggc cgaaccgtgt
     3121 ccggccggcc cagtcacgac attacagcaa taatgcatgc cgtacgtaaa gtacacatag
     3181 aggtgtcccg gcgggccgaa catgaccttg gtgcgcggtg tctccccacg ataactatga
     3241 ctggcaacgt cggtctgatc ataagcttcg gcctcgacga tccgtccgcg gaggatacgg
     3301 ccgtcaacct cacggaccag ttcacagccc aacaaacgag gcgctacttc ccaggctggt
     3361 ccgttcagaa aagtcgccag ttcagttgcc attcataacc tcctcctggc tagcggccac
     3421 gatgcgactt actaaaaacc ggctggcttt cggcgagatc atatgccgag cccggatagt
     3481 gtgcgccgta atgttggcat tgtcgacacc gaagatcgtt gtcggcttgc cgcggatcac
     3541 cagcatgtca aacataccgt aaataacatc cattggcatg gccagctgct taatatcttc
     3601 ggcggcagtt tggctcatga tagtattttc caggcttttg acgaaaggct gccaggtgtc
     3661 ctcagtcact tcaaaaccga cgatacgcgg agccaggcgt tcagccagac gagcattctc
     3721 tgggctgaac gtcggctgga ggcggataag ccgattgtag aaacgaaagt acaaattcag
     3781 ccgcacgcga taaagacgct tttggggcag accttcatag agtggcattt catagagcac
     3841 taagtgcttg accagatccg gcctgaggtg cgccatccgc accgccacca agcagcccat
     3901 agaatgccca accagtatca caggctcgcg taattttagc cgctccaagc tggctatgac
     3961 ggctgtgacg tggtcgtcaa ccgtgtagcg cagccattcg ggcttgggcg aggcgccgaa
     4021 accaagtaaa tcaaaagcca ctacccggta agatttagtc tgtagttgct ctaccacatg
     4081 tcgccatact tctcccgtcc ggccgatgcc gtgcaagagc actactggcg ggccttcacc
     4141 ttcgtcaatg gtacgtgcta aatagtatgg gcgccccagc gcccggtgcc agaaccggtc
     4201 gaaaaatctc aataccatct ctttattata gtctgcctat ctgtacaagc atgagctcga
     4261 aagctatact aaggcgtaac gatgcacagc ggcccaggca gcacagcact tattattatc
     4321 atcgtcataa tattaattat attttcggcg gtgtgttccg gcttgaacat tgccgtcatg
     4381 tcgctcgacc tggcggacct gaggcgaaag gccaaacttg gcaaccgagg agccaagcgg
     4441 gttttaccgc taaggaaaca agcgcaccta actctagcca gtatcttgct atccaatgtc
     4501 gccgctgttt cggccacttc actcgtactt gaccagcgcc tgaacggctg gctggccgga
     4561 ctactgggaa ccttattgat cgttatattc ggtgaagtta tgccacaggc tttattttct
     4621 aaaaatccac tggcctggtc cagccgcttt gcaccggtat tgcaggccat gatcgttgtc
     4681 acctatattg tcagcaagcc gctgcaagta ctacttgacc atctcttccc gcacgaacga
     4741 gccaagctac aaacccggca cgagctcggg ctgatgatca acgagcactt gagcgatgac
     4801 tctagcgagc tggatgaaga cgaggtggag attatgcgcg gcgccttgca actgagcgaa
     4861 aaacgcgtcc gagacattat gaccgatatt cgccacacat actggctggg ccttgatacg
     4921 gcgttggatg gccagaaaat cgatgagata aaagccgtcg gctacagccg cattccaatt
     4981 tttgatcacg ccctgaaaga atgctacggc ctactgctta tgaaagacct ggtcgatatc
     5041 gactttgacg aacgcaccct cttagtcaga gatttgctgc tccgccccac caagcttgtt
     5101 ggcagcatga cagccctgga cacaatgttc cggaagttca ttgccgctag tacacatctt
     5161 attcccatcg aaaaagacga ggagatcatc ggcatcgtta caattgaaga cttgattgaa
     5221 gaaatcatcg gccacgaaat ccaggacgaa accgatcggc agaaggactc gacaacccgg
     5281 atttgaggca tcggcaagag tgctgtttct cggccgccgc acgtctttcc atggtataat
     5341 tctccggtaa gtaatcatag tctcttactt tataaggagt ttatatttgg ttaagcaagt
     5401 agccgatgcg catcacgttt ggaataggca gctcattatc ataggcctgc cccttattat
     5461 cacactcgct ttcggcttcg gtctagcaca aatgcaattg cctaaaacca aggctcagca
     5521 aatcacacct tcggcttcat cgccacaatc cctacctccg gccgtcgaat catccaaatc
     5581 attaccgatg ttaccctttg caccaagcaa aaaactaccc tcggttacgt cttctagcag
     5641 tattgacagc aattcatcgc agaccgcgac ccctcagcct gccgcccaaa ccacgacaaa
     5701 cttacaggct gcagctactg ggtcacaacc cactagtccc tcaaattcgc aacaggattt
     5761 gccgcaagtc ctggacgttt tacactccat cagaaacata aatacccaac cgctgaatat
     5821 tagcgtcacg caaaatttgt tataagtatt tacttacgtt aagtaatcat gtatactgta
     5881 agttatgact gctttggaat cggatttaga agtgctagag cgtgctatgc gcatgttttt
     5941 ccagacaatg aaacggccgc agcattgggc tcgagtgtca gcgcggtccg ggctagcaat
     6001 tgaccgccct tcttcggcga tccttcacat cctgatgcat agcaagagcc acgcctgtcg
     6061 cgtacaggat gtcgcgcacc tactaggtgt cgaggctcca tcggtgacgc gcaagacgca
     6121 agagttggag ctcagcggct taattcgtcg tgtgccggat tcccgcgata aacgcgcggt
     6181 cgatttacgc ataactcccc gcggccgcac gattgccaag cgcctctggc aggctcagcg
     6241 cgaggtgatg tatcagacgc tcgccaactg ggacgaatcg gagcgacgta cctttgttaa
     6301 attatttgag cgctttagtc aggattatgt aagctccgtt gaagcagaac acctaaacaa
     6361 aaggaaggat taacgacaat gtcagaactc gcacaaactg ctgcgcccac cgagcaccac
     6421 gaaggtgaac gcagccatgc cgaaatcatg gtcatcatga ccgctttgat gctcgcttta
     6481 ttgctggccg ctctcgatca gactattgtc tcgacggctt tgccacggat tgccaatgac
     6541 ctgcatggct tgagcaaata ttcttgggtg gctactgctt acctgctgac aagtgccgtg
     6601 tcgacaccgc tgtacggcaa aattagtgat atgttcggac gcaaaaagat ttttcagagc
     6661 gctatcgtta ttttcctgat aggctcagcg ctatgcggcc tcagccagaa catgaatcag
     6721 ctgattgttt tccgggggct gcagggtctg ggcgccggcg gtctgatg
//
LOCUS       DATNGP010000148         7979 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_1367763, whole genome shotgun sequence.
ACCESSION   DATNGP010000148 DATNGP010000000
VERSION     DATNGP010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 7979)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7979)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7979
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1367763"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1339
                     /locus_tag="VN778_03805"
     CDS             <1..1339
                     /locus_tag="VN778_03805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50122.1"
                     /translation="DEHEQIITHTRYSTEPGDSAVGAHPFECRTTGLKLALNGELRGQ
                     SLAILAKKNDLDPRNFGNKTELLTNIIGSTLARREEKKALTVEETGKTLRRLFAQLEG
                     AFCLSIIFRDMSILVRDPWGRHPLSVGFLPDGKIVGASESMAFDDIGDQDDPHLPFLE
                     VRDVEPGEIIIRTSDTIQSFNIPRKVDHKLCPYEVIYIADERSVVYGAPVWQARYNMG
                     VELAKAEGAPPDVDIVVGVPNTGIASAQGYAEAHGKLFMPQAITKNPKYPKNMRTFIS
                     EGEAQQALLATKFIVDKSIVEGKKVASDDDSTIKGNVYRQTNGQLEEAGATEIHNRSS
                     SPPYRSPCTYGMDTTDVSKLVARGERTEEEVAKDLGAKSMRYNTPEAVEKAIDDAKND
                     FEAHIGKRTPSLLGRFCWECVSPVQVSPVDSLTTTIYAADHETQQEQELAKVA"
     gene            1389..2444
                     /locus_tag="VN778_03810"
     CDS             1389..2444
                     /locus_tag="VN778_03810"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF013568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HXR50123.1"
                     /translation="MNYQNPRVAETAELLKGRLPELENKAEILRAVELRALFDEIKTL
                     APEERGVFGKEVNVLRQELEGLVQASQIGAEQLSPIDVTAPFDVNTPADQQPKLLPSE
                     QGSTHPLMTELASVLDIFYRMGFTAVESREIDDDYHMFSSLNFPEGHPARDDYDTFVT
                     TQTDKDGKPFIAPAQTSTMQNRVLRKLKPNLEAGKPIAAVIPGRVFRNEDLDARHEHT
                     FYQLEGVYVGKNIHAGHLITTLKTFLQEYYGKELEVKTQPFYFPFTEPSFEFAISCPF
                     CDKKGCHICSQTGWIELLGCGMIHPNVLREAEIDLNEYTGFAWGMGIERLVMMKYGVE
                     DVRLFESGKLDFLRQFA"
     gene            2441..4969
                     /gene="pheT"
                     /locus_tag="VN778_03815"
     CDS             2441..4969
                     /gene="pheT"
                     /locus_tag="VN778_03815"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HXR50124.1"
                     /translation="MRVSVNKLRYINEHYDAGVNVVPDGVEALVERIGAQLGAVEAVI
                     PFGDKFSGALIVRVVECEKHPNADKLSLCKIDDGGTAEGVERDGQGRVQVVCGAPNVR
                     ADMLAVWLPPGVTVPSTFGTSDPFVLEAREIRGQKSNGMLASPKELGIGDSHEGILEV
                     DREVKPGTTFAKAYRTEGEVVLDIENKMFTHRPDCFGTMGIAREVAGIQQQAFKSPEW
                     YTAQPELPSVEGAKLPLTITNELPNLVPRFIAVSMSSITVKPSPVWLQIVLTEAGIRP
                     INNIVDYTNFYMLETGQPLHAYDYDKVKTLSGDKATLVIRNPKPGEQLALLNGKTITP
                     RPEAIMIAADKQLIGLGGVMGGSETEVDDSTKNIILECANFDMYSVRRTSMEHGLFTD
                     AVTRFTKGQSPLQNLAVLAKITSDLCEHFGGQVASSVVDDNHLDKTLLARNSIHPPVT
                     VAAEFINTRLGLQLSAADMASLLKNVEFTVEVKGEELIVTAPFWRTDIELREDIVEEI
                     GRLYGYDHLPLELPQRDLTPAPKDALLSLKAEIRERLVKAGANEILTYSFVHGDLLRK
                     AGQDPAHAFQIANALSPDLQYYRLSLMPSLLATVHPNLKAGYDEFVLFELGKAHIVGQ
                     NDDAGLPSEDNLTGLIVAAANKLKKPGAPYYLAKKYLQALVRDGLTYQPVPENMRAYD
                     ITKPYDLNRTAFIYVGEVFLGIIGEFRPAAARALKLPKHCAGFELDTTALLTVLDKAA
                     YTPLPRFPKVTQDISLKVPVDLAYQPLFDLVQAELDKVRPQNTLVELEPIDIYQRDDD
                     KEHKQISLRLHIASYERTLTDSEVNKLLNLAAAAAKATVGAERL"
     gene            5030..5572
                     /locus_tag="VN778_03820"
     CDS             5030..5572
                     /locus_tag="VN778_03820"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HXR50125.1"
                     /translation="MARKRDRTFAAFGAVLFLLTSSALTIAVVLTLVQQSKQNKASTS
                     TTNNSEANKVDNKLQGTKLTNFTPVTSIPQLQEIDTAPGTGETVKAGDTVTVDYTGAV
                     ASTGIIFQSSKDSGQPISFGLSQVIPGWSQGVPGMKVGGTRRLLIPAALAYGANPPAG
                     SGIPANADLVFDITLYKIGK"
     gene            5794..6042
                     /locus_tag="VN778_03825"
     CDS             5794..6042
                     /locus_tag="VN778_03825"
                     /inference="COORDINATES: protein motif:HMM:NF012675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HXR50126.1"
                     /translation="MVKNITIFTTNTCGYCQMVKKYLGAKGVTYEEVNLDEHPHRQAE
                     ALQMSGSMTVPVTIVTKTDDSKEVIVGYNLAKLAPAVA"
     gene            6049..7008
                     /locus_tag="VN778_03830"
     CDS             6049..7008
                     /locus_tag="VN778_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008579152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXR50127.1"
                     /translation="MSDTEATGKHDVIIVGAGPAALTAAIYTTREDIDTLLFERGVIG
                     GLAAVTDKIDNYPGFPDGIEGLTLADDLQKQAERFGAVIELGEVTAIHDEGDLKRLET
                     TSGDLYARAVLVATGSDYKKIGVPGEQEYYARGVHYCATCDGAFYRDKRLVVVGGGNS
                     AIQESIFLTRFASHIDLLVRSTIKASDVLQHELDEYVKQGKITVHLGTTTDKIVGENN
                     GVTKVIGTKDGKKIEFETDGVFVFVGLMPNSQFLKNSAIRLDEIGFVSTDQHLQTAIP
                     GVFAAGDIRSGATMQIASAAGEGATAALKIREYLEGRPHPVQN"
     gene            7064..>7979
                     /gene="cfa"
                     /locus_tag="VN778_03835"
     CDS             7064..>7979
                     /gene="cfa"
                     /locus_tag="VN778_03835"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: protein motif:HMM:NF008686.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HXR50128.1"
                     /translation="MDHARKTVTDLLTLADIKTNGDRPFDIQVHDDRFYRRVLSQREL
                     GLGESYMDGWWDALRVDELIAHILSTNIRSKIIFSPALLAAFVRSTAANRQNFRRAPK
                     NASRHYNIGNDLYQRMLGQRMIYSCAYWKDAKTLDEAQEAKLDLICRKLHLKKGMSLL
                     DIGCGWGGFAEFAANKYGVEVTGITPAAEQVKLAKERTRGLPVTILQQDYREVTGSFD
                     RIVSIGMLEHVGPKNYRTFFLKNHALLKDGGLMLHHFIGRNKSVRITDPWIDRYIFPG
                     GVIPTLAQISRALERLLIAEDVHNFGPYY"
BASE COUNT         2061 a         1982 c         2101 g         1835 t
ORIGIN      
        1 tgacgaacat gaacagatta ttacgcatac tcgctacagc acggagcctg gcgatagcgc
       61 ggtaggagca catcctttcg agtgtcggac cactggccta aaactggctt taaatggtga
      121 attacgaggg caaagcctgg ctatcctagc taaaaagaac gatctagatc ccagaaattt
      181 cggtaacaaa actgagctgc tgaccaacat tattggctcc accctagcgc gacgtgaaga
      241 gaaaaaggct ttgactgttg aagaaaccgg taaaacactg cgcaggttat ttgcccaact
      301 ggaaggtgca ttctgcttgt caattatatt tagggatatg tcgatcctgg tgcgggatcc
      361 ctggggaaga caccccctct cggttggctt cttaccggat ggcaaaatag ttggcgcgtc
      421 cgaaagtatg gcatttgacg acattgggga ccaggatgac ccccatctgc cgtttttaga
      481 agttagggat gttgagccag gggaaatcat tattagaact tcagatacaa ttcaaagttt
      541 taacatcccg aggaaagttg accacaagct ctgcccctat gaagttattt atatcgccga
      601 tgaacgcagt gtcgtatatg gagcaccggt gtggcaggct cgctataaca tgggcgtaga
      661 gctcgccaaa gccgagggcg cgccgcccga tgtcgacatc gtggttggcg taccgaacac
      721 gggcatagct tctgctcaag gttatgcgga agcacatggc aagctattta tgccacaggc
      781 aattaccaag aaccctaagt acccaaaaaa tatgcggact tttatatctg aaggagaagc
      841 ccaacaggca ctattggcaa cgaagtttat cgttgacaaa tcaattgtag agggcaagaa
      901 ggtggcttca gacgatgact cgaccataaa agggaatgtt tatcggcaaa caaacggcca
      961 acttgaggag gctggcgcta cggaaataca caatcgctct tcttcgccgc cgtatcgaag
     1021 cccgtgtacg tacggcatgg acaccacaga cgtcagcaaa ctagtagctc gcggtgagcg
     1081 aacagaggaa gaggttgcta aggatttagg cgctaagtct atgcgctaca acacaccgga
     1141 agcggtggaa aaagccatcg atgatgccaa aaatgacttt gaagctcata ttggcaaacg
     1201 aacccccagc ctacttggca ggttctgctg ggaatgtgta agccccgtac aagtcagccc
     1261 agtcgacagc ctgaccacta caatctatgc ggccgaccac gaaactcaac aagagcaaga
     1321 attagctaaa gttgcttagt gtaactgtta acacataagc ggttgcactg ctatactgtt
     1381 tttaagatat gaactatcaa aatccgcgcg ttgctgaaac tgctgaactg ctgaaaggcc
     1441 ggttgcctga actggaaaat aaggctgaga ttttgcgggc ggtggaactg cgggcgttgt
     1501 ttgatgagat caagactttg gcgccagaag agcgcggtgt ttttggcaaa gaagtgaatg
     1561 tgctgcgtca agagttagaa ggcttagtgc aggcgtcgca gattggggcc gagcaattgt
     1621 cgccgatcga cgtgactgca ccctttgacg ttaacacgcc tgctgaccag cagccgaagc
     1681 tcctaccaag cgagcaaggc agcacccatc ctctcatgac cgagctggca tcggtgctcg
     1741 acatctttta tcgcatgggc ttcacggctg tggaatcgcg tgagatcgat gacgactatc
     1801 atatgtttag ctcgctaaac tttcctgagg ggcacccggc ccgcgacgat tatgatacct
     1861 ttgtaaccac tcagacggac aaggacggca agccatttat cgcgccggca caaacatcta
     1921 ccatgcaaaa ccgagtgctt cgtaagctaa aaccaaatct cgaagctggg aagccgatcg
     1981 ctgccgtcat ccccggccgt gtcttccgca acgaagatct cgacgcccgc catgagcaca
     2041 ccttctatca actggaaggc gtgtatgtgg gcaaaaacat tcatgccggg catctaatta
     2101 caacgctgaa gacttttttg caggaatatt atggcaagga gctggaggtt aaaacccagc
     2161 cgttttactt ccccttcacc gaaccctctt tcgagtttgc gatcagttgt cctttttgcg
     2221 ataaaaaggg ctgccatatc tgttcgcaga ccggttggat tgagttactc ggctgcggca
     2281 tgattcatcc caacgtactg cgagaggccg agattgacct caacgagtac accggtttcg
     2341 cttggggtat gggtatcgag cgcttagtga tgatgaagta cggcgtggaa gacgtccggt
     2401 tgttcgaaag cggcaagcta gactttttga ggcagttcgc atgagagtca gcgttaataa
     2461 attacggtat atcaatgagc attatgacgc aggggttaat gtagtcccgg atggcgtcga
     2521 ggctttagtc gaaagaattg gagcccagct gggtgctgtc gaggcagtaa ttccgtttgg
     2581 cgacaagttc agcggcgccc tcattgttag ggttgttgaa tgtgagaaac atccgaatgc
     2641 cgacaagctt agtctttgta agatagatga tggcggcact gccgaaggtg tcgaacgtga
     2701 tggtcaagga cgcgtccagg tcgtctgcgg cgcaccaaac gttcgcgccg acatgctagc
     2761 cgtgtggttg ccgcctggcg tgactgttcc cagtacgttt ggcacgagcg acccgtttgt
     2821 tttagaggcg cgtgagattc gggggcagaa aagcaatggc atgctggcta gtcccaaaga
     2881 acttggtatc ggtgacagtc atgaagggat tttggaggta gatcgagaag tcaagcccgg
     2941 taccacgttc gctaaggctt atcgcactga aggcgaagtc gtgctcgaca tcgagaataa
     3001 gatgttcacc caccggcctg attgcttcgg taccatgggc attgctcggg aggtggccgg
     3061 catccagcag caagccttta aaagtcctga gtggtatacc gcgcagcccg aattacctag
     3121 cgtcgaaggt gcaaaattgc ctctcacgat aaccaatgaa ttgccaaacc tcgtaccacg
     3181 atttattgcc gtatcgatga gcagtatcac agtcaagccc agcccggtgt ggctgcaaat
     3241 tgtcctcacc gaagccggca ttcgcccgat caataatatc gtcgattaca ccaactttta
     3301 catgctggaa accggccagc cactgcatgc ttatgattat gacaaggtga agacactgag
     3361 tggtgataag gcaacgctgg ttatccgtaa tcctaagccc ggcgagcagc tagcacttct
     3421 aaacggcaaa acgataacgc cgcgacccga agccattatg atcgccgcgg acaagcagtt
     3481 aatcgggctc ggcggcgtca tgggcggcag tgaaaccgaa gtcgatgatt caaccaaaaa
     3541 catcatcctg gaatgcgcca acttcgacat gtattccgtg cgccgcacca gtatggagca
     3601 tgggctattc acggatgcgg tcacgcgctt taccaaaggg caatcgccgc tacagaatct
     3661 ggctgtacta gccaaaatta cgagcgacct ttgtgaacat tttggcggtc aggtggctag
     3721 ctctgtggtt gatgacaatc acctcgataa aaccttgctg gcccgcaact ctatccatcc
     3781 gccggtaaca gttgccgcag aatttattaa tactagactg gggctgcaac tttcagcggc
     3841 cgacatggcg agcttgctga aaaatgtcga gtttaccgtt gaagtaaaag gtgaagagct
     3901 catagtgacg gcgccatttt ggcggaccga catcgaactg cgtgaagaca tcgtggaaga
     3961 aatcggtcgc ctgtatggct atgatcactt acccctagag ctaccgcagc gcgacctgac
     4021 gcctgccccg aaagacgctt tattgtcgct caaagcagaa atccgcgagc gcctcgtgaa
     4081 ggctggcgct aacgaaattc tgacctacag ctttgtacac ggcgacctgc ttcgaaaagc
     4141 cggtcaagat ccggctcatg ccttccaaat cgctaacgcc ttgagccctg acttgcaata
     4201 ctatcgcctt agcttaatgc caagtttgct ggctacggtt cacccgaacc ttaaggctgg
     4261 ctatgatgag ttcgtactct ttgagttggg caaagcccac attgttggtc agaatgatga
     4321 cgctgggcta ccgagcgaag ataatttgac tgggttgatt gtggccgctg ctaataagct
     4381 gaaaaagccg ggcgcgcctt actacttggc caaaaagtat ctgcaagcgc tcgtgcggga
     4441 cggcctgacc taccagcctg tgcccgaaaa catgcgggct tacgacatta caaaaccgta
     4501 tgacctgaac cgcaccgcct tcatctatgt cggtgaggtg tttttgggaa ttatcggtga
     4561 gtttcggccg gcggcggcga gagctctaaa gctgcccaaa cactgtgccg gcttcgagct
     4621 agatacgaca gctctcttaa cggtgctcga caaagccgcc tatacacccc tgccgcgctt
     4681 tccgaaagtt acacaggata tatcgttaaa agtgccggta gacctggctt accagccgct
     4741 ttttgacctt gtccaagccg aacttgataa agtccgcccg caaaatacct tggttgagct
     4801 tgagcccatc gacatttatc aacgtgatga cgacaaagag cacaaacaga tcagcttgcg
     4861 cctgcatatt gccagttatg agcgcacgct gaccgatagt gaagttaata agttactcaa
     4921 tctagctgcc gctgcagcaa aagccacagt cggtgccgag cgcctttaga aatcaaactt
     4981 aaagtgcggt ataattagga ctaattgatg agaaattttt ggaggatgta tggctcgaaa
     5041 gcgtgatcgc acttttgccg cgttcggggc ggtgcttttt ttgctgacct cgtcggctct
     5101 gaccatcgcg gtagttttaa cactagtaca acaaagcaag caaaataaag cgtcaaccag
     5161 tacaactaat aattcggagg ccaataaagt ggacaataaa ttacaaggta cgaaactaac
     5221 taactttacc cctgtaacta gcatcccgca gctgcaagag atcgatacgg cgcccggcac
     5281 gggtgaaacc gtcaaagccg gcgataccgt aactgtcgat tacacgggcg ctgtcgcctc
     5341 gacaggtatt atttttcaaa gctctaaaga cagtggtcag ccaatcagct tcggcctcag
     5401 tcaagttatt cccggctggt cgcagggcgt gcctggtatg aaagttggcg gcacgcggcg
     5461 cttgctcatt ccggcggccc tggcttatgg cgccaatcca ccggccggct ctggtattcc
     5521 agccaacgcc gatctggttt tcgacatcac gctatataaa atcggcaaat aggcttcact
     5581 cgtcattcgc tctcctgtgc aaattcggag taaaaagcac aatatgtagc gtattgcttt
     5641 tgactcaagc gctatatata atctttctta cagtcagatt gttcttaggc aagtacacta
     5701 aaaacacata aaataaaagc gcggaaaatg actgtaagcc gtacctgaga gacacggcga
     5761 ggtatttcta gcgcttctaa cgagggggac cgaatggtca aaaatatcac catttttact
     5821 actaatacct gtggctactg ccagatggtt aaaaaatatc tgggtgccaa aggcgtgacg
     5881 tatgaagaag tcaatctcga tgagcacccc caccgccaag ccgaggcttt gcagatgtcg
     5941 ggttctatga cggtgccagt caccatcgtt accaaaactg atgattcgaa agaggtcatc
     6001 gtaggctata acttagctaa actcgcccca gctgtggctt aattccaaat gagcgatacc
     6061 gaagcgaccg gaaaacatga tgtgattata gtgggcgcgg gcccggcagc cttgacggcg
     6121 gctatttaca cgacgcgaga agacattgac acgttgctgt ttgagcgtgg tgtaattggc
     6181 ggcctggcgg ccgttacgga caagattgat aattatccgg gcttcccgga tgggatcgaa
     6241 ggactaacgc tagccgatga cctgcaaaag caagctgagc gttttggcgc ggtcattgag
     6301 ctgggcgaag tcacagcgat tcatgacgaa ggcgacttaa agcggctgga aacgaccagc
     6361 ggggacctgt atgcccgagc agtactggtt gccaccggta gtgactataa gaagatcggc
     6421 gtgcccggcg agcaggaata ctacgctcgc ggtgtccatt actgtgctac ttgcgacggc
     6481 gccttttatc gtgataaacg tctggtcgtg gtgggcgggg gcaattcagc tattcaggaa
     6541 agcatctttt tgacgcgctt cgcgtcccac atcgatctcc tagtccgctc cacgattaaa
     6601 gccagcgatg tgctccagca cgagctggat gagtatgtga aacagggcaa aatcactgtt
     6661 catctgggta cgacaactga caaaatcgtc ggtgaaaata atggcgtgac gaaagttatc
     6721 ggtacgaagg atggtaaaaa gatagaattt gaaactgacg gagtcttcgt ttttgtcggc
     6781 ctcatgccta attcgcaatt tctgaaaaac tcagctattc gtttggatga gatcggcttt
     6841 gttagcaccg atcaacactt acagacggct attcccgggg tctttgcggc cggtgacatt
     6901 cggagcggag ccaccatgca aatcgcctcc gcggccggcg aaggcgcgac cgcggccctc
     6961 aaaattcggg aatatctcga aggccgcccg catcccgtgc aaaattaatt cactgtagtc
     7021 tttatcacag tgggcgtccg agccggcggt gtaaagtgtt ggcatggatc atgcgcgaaa
     7081 gaccgtcact gacctactga ccctggcaga catcaaaaca aacggcgacc ggccatttga
     7141 tatccaggta cacgacgacc gcttttatcg gcgggtgctt agtcagcgtg agctgggcct
     7201 gggcgagagc tacatggatg gctggtggga tgccctacgg gtagatgagc ttattgccca
     7261 catactctcg accaacatcc gcagcaaaat tatcttttcg ccggcgttat tagcagcttt
     7321 tgtgcgctct acggccgcca accgacagaa tttccggcga gctcccaaaa acgcttcgcg
     7381 ccactacaac atcggcaatg acttgtatca gcgaatgctc ggccagcgaa tgatttacag
     7441 ctgtgcctac tggaaagatg ccaagacgct tgatgaagcc caggaggcca aacttgacct
     7501 tatttgccgc aagctccacc tgaaaaaggg tatgtcactg ctcgatattg gttgtggctg
     7561 gggcggtttt gccgagttcg ctgctaataa atacggagta gaagttacgg gaatcactcc
     7621 ggctgctgaa caggtgaaac tagctaagga acgcaccaga ggcttgccgg taaccatctt
     7681 gcagcaagat tatcgagagg tgactggttc attcgaccgt attgtgtcga tcggtatgct
     7741 ggaacatgtc ggccccaaaa attatcggac gtttttcctg aagaatcatg ccttgctgaa
     7801 agatggtggc ctaatgctac atcacttcat tggccgtaat aaatcagttc gaataaccga
     7861 tccgtggatt gaccgctaca tttttcctgg tggtgtgatt ccgaccctcg cccagatcag
     7921 ccgagccctt gaaagattat tgattgctga agacgtgcat aatttcggcc cgtactacg
//
LOCUS       DATNGP010000149         3371 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_466092, whole genome shotgun sequence.
ACCESSION   DATNGP010000149 DATNGP010000000
VERSION     DATNGP010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3371
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_466092"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            122..2068
                     /locus_tag="VN778_03840"
     CDS             122..2068
                     /locus_tag="VN778_03840"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HXR50129.1"
                     /translation="MVLSNGQLFVGLDENGLVHDFYYPYVGLDNLTNARSLWHKIGVW
                     VDGSFSWTDSGNWQTSVNFSADGLIGSVRWSSSTLGVSIETQDFVDSESNTFIRCMNV
                     TNLTDKSHDVRVFMHQIFQISRAGRADTAMYVPDDHYILDYKGRCCLLIGGVFRDGGS
                     FDQYAVGNYGIEGKDGTFRDAEDGVLSGNAVEHSDVDSVIRFQRNLLGRQSCQIDYWV
                     VAADSQSDAQAIHLALKTTGVDNRYERTKTYWENWLKPALATANRLEGRQATELKKSL
                     LIIKAHCDARGSILASGDSSIFNYGRDYYCYCWPRDAAYAIWPMMRVGLFEEAKRFFD
                     FARDTMHPDGYLMHKYQPDRAIGSTWHPLLHNKARELAIQEDETAIVLFMLGEYFRMS
                     RDKTYIETIFNSFIKPCADFMTNFVDAATGLPHASYDLWEQLFVTSTYTTCTVIAGLE
                     TAANLAEVINQAQSDAELWRQAAQSMRGKLSQLYHPSGYFRKGLLVQPDGSIQYDDKL
                     DISNLYGPFMFAKLALDDERLVNTLQAVERGLLNTSPIGGVVRDEHDSYFLSKIQYAG
                     NPWAVCTLWLAQVYNALGNTEDAGKLLGWALDRAYPSGALSEQFDPETGLALSVAPLV
                     WSHAEFINTTLDMLKVADQQQRTK"
     gene            complement(2156..2359)
                     /gene="rpmG"
                     /locus_tag="VN778_03845"
     CDS             complement(2156..2359)
                     /gene="rpmG"
                     /locus_tag="VN778_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007746456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HXR50130.1"
                     /translation="MAKKNDKRKLVGLVSEESGERIYYTSKNTMNTPDKLSLKKYSPK
                     LRRVVTFVETKKNLGRNEVKPKK"
     gene            2530..3045
                     /locus_tag="VN778_03850"
     CDS             2530..3045
                     /locus_tag="VN778_03850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50131.1"
                     /translation="MPTVDVQELRATERAEVARLTAQGEAGLNELRMQRVCDYVAWLA
                     GNTQAQNPMDELTQQQLVGDVFLSLARQADRVIDRPNSQRLLEIETAMGVYDNAEVFA
                     TALKALKRLRPKTRAAEDIKRFIQAYHSLLARSLITMATEQECRKLEQSVGSTQIISG
                     RQRHYGRENEH"
     gene            complement(3194..3361)
                     /locus_tag="VN778_03855"
     CDS             complement(3194..3361)
                     /locus_tag="VN778_03855"
                     /inference="COORDINATES: protein motif:HMM:NF015957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HXR50132.1"
                     /translation="MAGVCGGIAEYLNIDPLVVRLIFVALLLFGIAPIILIYLIMWVV
                     VPDNPAEIKSA"
BASE COUNT          814 a          897 c          870 g          790 t
ORIGIN      
        1 atgcgtttcc ctcattccca tgtaaacggg aatccagaca agcaattggt gctcctcttc
       61 tcgctggatc cccgatcggg tcggggatga cgagccagga gggtctgtag tgggacgacc
      121 tgtggttctc tcaaatggac agctttttgt cggactcgac gaaaacggct tggtgcacga
      181 cttctattat ccgtatgttg ggctcgacaa cctcacgaac gcgcggagtc tatggcacaa
      241 aataggcgtg tgggtggacg gtagttttag ctggaccgac agtggcaatt ggcaaaccag
      301 cgttaatttt tcggccgatg gcttgatcgg ttcggtgcgc tggagttcgt cgactctagg
      361 tgtcagtatc gagacccaag actttgtcga cagcgaatcc aacactttta ttcgctgcat
      421 gaatgtgacc aatcttaccg ataaatctca tgatgtacgt gtctttatgc accagatttt
      481 ccagatctca cgggccggcc gggctgacac ggccatgtat gttccggacg accactatat
      541 tctcgattat aaggggcggt gctgcctgct gatcggcggc gtattccggg atggtggcag
      601 cttcgaccag tatgccgtcg gcaactacgg tatcgaaggc aaagacggga cttttcgtga
      661 cgctgaagat ggggtcttgt cgggcaatgc ggtcgaacac agcgacgtcg acagtgttat
      721 ccgctttcag cgcaacctat tgggccgcca gtcgtgccag atagattatt gggtcgtagc
      781 cgccgatagc caaagtgacg cccaagcgat tcacctggcg cttaaaacaa cgggggtgga
      841 caaccgctat gaacgcacca aaacgtactg ggaaaattgg ctcaagccag ctttggcaac
      901 cgccaatcgg ctcgaagggc gccaagcgac tgaactcaag aaaagcctgc taataatcaa
      961 agcacactgt gatgcgcgtg gctcgatcct cgctagcggc gactccagta ttttcaatta
     1021 tggccgtgac tactactgct actgttggcc gcgagacgct gcttatgcaa tctggccaat
     1081 gatgcgcgtc ggcctgttcg aagaggccaa acggtttttc gattttgccc gcgacaccat
     1141 gcaccccgac ggctacctga tgcacaagta ccaacccgat cgggccattg gcagcacctg
     1201 gcacccgctg cttcacaata aggcacgcga gctggcaata caggaggatg agacggcaat
     1261 cgtgctattc atgctcggtg aatatttccg tatgagccgc gataagacgt acatcgaaac
     1321 gatatttaac agctttatca agccgtgcgc tgactttatg accaattttg ttgatgccgc
     1381 taccggtctg ccgcacgcca gttacgacct gtgggagcag ttattcgtca cctcaacgta
     1441 caccacctgt accgtaatcg ccgggctaga aaccgccgct aatctggctg aggtaatcaa
     1501 ccaggcccaa agcgacgctg agttgtggcg ccaggctgcc cagagcatgc gcggcaaact
     1561 gtcacagctc tatcacccaa gcggctactt taggaaaggc ctgctggtcc agccagatgg
     1621 ttctatacag tatgacgaca agctcgacat atctaacctc tacgggccgt ttatgtttgc
     1681 caaattagca ctcgacgatg agcgcttggt caatacgctt caggctgtcg agcgcggcct
     1741 attgaacact tcgccaatcg gcggcgttgt ccgtgacgaa catgacagct acttcctcag
     1801 taaaatccag tacgcgggta atccgtgggc ggtgtgcacg ctgtggctgg cgcaagtcta
     1861 caatgcgctg ggcaataccg aagatgctgg caagctgctc ggttgggcgc tcgaccgggc
     1921 gtacccgagt ggtgccctga gcgagcaatt cgatccagaa accggcctgg cgctcagtgt
     1981 tgcgccgctt gtctggagcc acgctgagtt catcaacacc acgctcgata tgctcaaagt
     2041 cgccgatcaa cagcaacgaa cgaagtagct aatagcctac ggccgcaagc taatagcaaa
     2101 aaacagccag cttgttcggc tagctgctat cagctaatag ttataagcta tttggctatt
     2161 tcttgggttt gacttcgttg cggccgaggt ttttcttggt ttccacgaaa gtcacgacgc
     2221 ggcggagctt tgggctgtat ttcttgagcg acagcttgtc cggcgtgttc atcgtgtttt
     2281 tcgaggtgta gtaaatgcgc tcaccagatt cttcgctgac gagtcctacg agttttcgct
     2341 tatcattttt ctttgccata tgctccgatt ctaacagatg aggttatcgt tgtaaacaga
     2401 tggcaacgta atcattcccc aggcttatca cacagattgc ccggtgaagc cagtaatttg
     2461 atttaagccg cacagtatag tattatccta gttcacacgg ccaagaaccc tgacaacaac
     2521 gacataccgg tgcccacagt agatgttcaa gaactgcgtg ccacagaacg tgccgaggtt
     2581 gcccgcctca cagctcaggg agaagcgggc cttaacgagc tccggatgca acgcgtgtgc
     2641 gactacgtcg cctggcttgc gggcaatact caagctcaga atcccatgga tgagcttact
     2701 caacagcagc tcgttggaga tgtcttcctg tcgcttgcca gacaagcaga cagggtgatt
     2761 gaccgtccca acagccagcg gcttcttgaa atcgaaactg ccatgggcgt atatgacaac
     2821 gccgaggttt ttgctaccgc actaaaggcc ctaaagcgcc tgcgccctaa gacgagagcc
     2881 gccgaggaca taaaacgatt tattcaggct tatcatagct tgttggcgcg tagtctgatc
     2941 actatggcaa cagaacaaga atgtcgcaaa cttgagcaaa gtgttggatc gacgcaaatc
     3001 atatcgggtc ggcaaagaca ctacggacga gagaacgagc attagtgttc ccacgaaaca
     3061 acaaaaatac cgggtaatta ctccggtact tttgttactc ttgcaagaag tatgataact
     3121 cttttgtaag tttcaggatt ttttgttcct gattcctacc tacttcggat gctacatcgc
     3181 gctcatgctt ttgtcaagca cttttaattt cagctggatt gtccggcacc acgacccaca
     3241 ttatcagata gatcaagata atcggggcga tcccaaacag caaaagtgcc acgaaaatca
     3301 gccggacaac cagcgggtca atattgagat actcggcaat ccccccacat acccccgcaa
     3361 tccgcctatc c
//
LOCUS       DATNGP010000150         3924 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_2105860, whole genome shotgun sequence.
ACCESSION   DATNGP010000150 DATNGP010000000
VERSION     DATNGP010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3924
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2105860"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(97..408)
                     /locus_tag="VN778_03860"
     CDS             complement(97..408)
                     /locus_tag="VN778_03860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50133.1"
                     /translation="MALREELQTLSREQAIVATQAVIDRLNRGEAEQAVMDHVLDNDY
                     QAFAAAVNFLYETSIDGGLDSTEAAFMAIGARTLLHIQRELAEQQLFAPEDLSELSEE
                     S"
     gene            551..1129
                     /locus_tag="VN778_03865"
     CDS             551..1129
                     /locus_tag="VN778_03865"
                     /inference="COORDINATES: protein motif:HMM:NF016214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HXR50134.1"
                     /translation="MTARTHDLAAITALGVVVLVEPLRTVTLATGIVAVFANLIGGIT
                     PDIDQPTAPFWRNLPIGRFFGRIFDDLSGGHRFLTHSILGLALFGFAAHWLLVFLQPI
                     MKNVNIGLVWWAFMIGMASHLVMDTLTKEGVPWLLPIPIKFGLPPVKAFRVTTGKAVE
                     TFGVFPALLVFNAWLYYVNYHRLLVILHHYLV"
     gene            1194..2318
                     /locus_tag="VN778_03870"
     CDS             1194..2318
                     /locus_tag="VN778_03870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HXR50135.1"
                     /translation="MKKLWKLVALIGVGVAALGGVAFAASNSTVNVSGTSNHSVYKAG
                     QSVTITGTVNGDIVCVGQTIDIDATVNGDVVCAGQSVTVDGTVHGNVRVAGQTVNIGA
                     KIDHNASLAAQTATLQNNASVDGDVGLAAQSANVNGSIGRDLTMAVHTATLTNSVGRN
                     VDAHANNRLQLADGANVKGNLTYTSPNTLQKAAGAVVGGKVAYHKATVHHHHIAWGGI
                     GIGVGLFLSLTFLIFALVLVALFPQTFRDWNRVAARRIWIVLLTGFLAMFIIPAVVLV
                     LVITVIGWPLAFFFSLLWILALLLSGPLAAFFVGSLIFRRDRHPVLMVLVGSIVLGII
                     SLIPILGWIVDLLAIWFGTGSALVNLRATYRRPEYATKPN"
     gene            2322..3074
                     /locus_tag="VN778_03875"
     CDS             2322..3074
                     /locus_tag="VN778_03875"
                     /inference="COORDINATES: protein motif:HMM:NF039315.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5996 family protein"
                     /protein_id="HXR50136.1"
                     /translation="MSKLPPLSLRELQPTRDYLQDVAKVLGKLQQAFLPVESHDWQRG
                     LVVNMRGLSTQPFMVASQETRASIDLVKHKVRLDGSKWLLEDYSAQEMMNNVRAWLES
                     RGLQVHIEEPEFSESTPHFDSSQADKYATALWWMDQQLQALKTDLKTGLVSPVLLYPH
                     HFDLSLTWFPESDERQLTVGWSTGDATIAEPYVYVTAYPEPEGFTDQQLPAPAYWNEK
                     DFSAAILLYRDLAQAAEPEKLFASFAELLKAL"
     gene            3117..3842
                     /locus_tag="VN778_03880"
     CDS             3117..3842
                     /locus_tag="VN778_03880"
                     /inference="COORDINATES: protein motif:HMM:NF014582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HXR50137.1"
                     /translation="MAPILLSIATFISTLSGGLTGLKNQDRLHRFLGYTAGVLLGVVT
                     FDLLPEIFKTLSAQHRSATGAMIALVCGFLLFHIIEKSILIHHMNEKEYGHHHHPHVG
                     VASALALSGHSFLDGVGIGLGFQASSTVGIAVAIAVIAHDFTDGLNTVNLMLLNNNKS
                     RKAFMFLLLDATTPILGVLSTKLFTVSGNALTLYLGFFAGFLLYIGASEILPEAHSEH
                     SSYQTIGLTIVGVVFIFIVTRFA"
BASE COUNT          863 a          972 c         1062 g         1027 t
ORIGIN      
        1 ttgatttcgt aaatactgct ctgtatttgt cagctataac cttatgccgc acctctgaga
       61 aaagttgcac tttcatgaat aatgctgtac caaaaactaa ctttcctcag aaagttcgga
      121 cagatcttcg ggggcgaaaa gttgctgctc ggcgagttcg cgttggatgt ggaggagggt
      181 gcgggcgccg atggccataa aagcagcttc cgttgaatcg agaccgccgt cgatggaggt
      241 ttcgtaaagg aagttgacgg ctgctgcaaa ggcttggtag tcgttatcca aaacatgatc
      301 catcactgct tgttcggctt caccacggtt cagccgatct ataacggcct gggtggcgac
      361 aatagcctgt tctctactta gggtttgtaa ttcttctctc agcgccatgg ttactcctcg
      421 tttttatcag caaagcctag ttgcaaacta ggcttttact ttgccataac atcatgctag
      481 caaatagcaa taaaaaacaa aatacattta tataaagtca agcccaagaa gtttgataaa
      541 cttaaacagt atgacagcgc gaacgcacga cctagcagct attaccgccc tcggagtggt
      601 ggtgcttgtg gagccgctca ggacggtgac tttggcgacg gggattgtgg cggtgtttgc
      661 gaatctgatt ggggggatta cgccggatat tgatcagccg acggcgccgt tttggcggaa
      721 tttgccgatt gggcggtttt ttgggcggat ttttgatgat ctgtcgggtg ggcatcggtt
      781 tttgacgcat tctattctgg ggctggcgct gttcgggttt gcggcgcact ggctgttggt
      841 atttctgcag ccgatcatga agaacgtcaa tattgggctg gtgtggtggg cgtttatgat
      901 tgggatggcg agccatttgg tcatggatac tttgacgaaa gaaggggtac cgtggctgct
      961 gccgattccg atcaaatttg gtttgccgcc ggtgaaggca tttcgcgtca ccactggcaa
     1021 agctgtcgag actttcggtg tttttccggc gctactggtt tttaatgcct ggctgtatta
     1081 cgtgaattat caccggctgc tggtgatctt acatcactac ttggtgtagc taaacttact
     1141 gtgcgctgcc tgataaatgc gggtataata ccagcaaata ggaggtgccg agtatgaaga
     1201 agttgtggaa attggtggct ctcataggtg taggggtggc ggcactgggt ggcgtagctt
     1261 tcgcggcgag caatagtacg gtaaatgtca gtggcacgtc aaatcatagc gtgtacaaag
     1321 ctgggcagtc ggtgacgatt accggcaccg ttaacggcga tatcgtgtgt gttggccaga
     1381 cgattgatat cgatgccacg gtcaatggag acgtggtctg tgcgggccag tccgtgactg
     1441 ttgacggtac cgtacatggc aatgtgcgcg tcgccggcca aacagtcaat attggagcta
     1501 aaattgatca taacgcttcg ctcgcggcgc agaccgcgac gctgcaaaac aatgcttcgg
     1561 tcgatgggga tgtcggcctg gctgctcaaa gcgccaatgt caacggctcg attggccgtg
     1621 acctgaccat ggccgtccac accgccacgc tcacaaattc cgttggccgc aatgtcgacg
     1681 cccacgccaa taaccggctg cagttagccg atggagccaa tgtgaagggc aatctcacct
     1741 ataccagccc gaataccctg cagaaggctg cgggtgccgt tgtcggcggc aaggtggcgt
     1801 accataaagc caccgtgcac caccatcaca ttgcctgggg cggcattggt atcggcgtcg
     1861 gtctattctt gagcctgaca ttcttgattt ttgctctcgt cttggtcgcg ctcttcccac
     1921 aaactttccg agactggaac agggtggcgg cgaggcgcat ctggatcgta ctgcttaccg
     1981 gcttcctagc catgttcatc atcccggcgg tcgtgctggt actggtgatc accgtcatcg
     2041 gttggccgct agcctttttc ttcagcttgc tgtggatact agcactcctt ctgagtggtc
     2101 cgctggcggc atttttcgtc ggcagcctca tcttccgtcg tgatcggcac ccggtgttaa
     2161 tggtcttagt aggtagtatc gtactcggaa tcattagctt gattcctatc ctcggctgga
     2221 tcgtagactt actagctata tggttcggaa cgggtagcgc gctcgtcaac ctccgggcaa
     2281 cgtatcgtcg gccggaatac gccacgaagc ccaactgatc tatgagtaag ttgccgccgt
     2341 tgtctttgcg agaattacag ccgacacgcg actatctgca agatgtcgcc aaagtgctag
     2401 gaaagctaca gcaagctttt ctgccggtgg agtcacatga ttggcagcgc gggttagtcg
     2461 tcaatatgcg cgggctgagc acgcagccgt ttatggtggc cagccaggaa acgcgcgcca
     2521 gtatcgatct ggtgaagcat aaggtacgcc tggatggcag caaatggctg ctcgaagatt
     2581 atagtgctca ggaaatgatg aataacgtac gggcttggct cgaaagccgc ggactgcaag
     2641 tccacatcga ggaaccagag ttttccgaga gcacacctca ctttgattca tcccaagccg
     2701 ataaatatgc cacagccctg tggtggatgg atcagcagct ccaggcactg aaaacagatc
     2761 tgaagacggg tttagtcagc ccggtactgc tatatcctca tcattttgac ctgtccctga
     2821 cttggttccc ggagagtgat gagcggcaac tgactgttgg ttggtcgaca ggcgatgcaa
     2881 cgattgctga gccgtacgtg tatgttactg cttatccaga acccgagggg tttactgatc
     2941 agcagctgcc ggccccggct tattggaatg agaaagattt cagcgctgcc attttgctct
     3001 accgcgattt ggctcaagcc gctgagcccg aaaagttgtt tgcctcattt gccgagctac
     3061 ttaaagcact gtgacgaatt tgcaataact tatcaaagta gcttacactg acgctcatgg
     3121 cgcccatttt actatcgatc gctactttca tttctactct gagcggtggc ctgaccggcc
     3181 tgaaaaacca agaccgttta caccgctttt tgggctatac agccggtgtt ttgcttggcg
     3241 tcgtcacgtt cgacctcctg cctgaaattt tcaaaacctt gagcgcccag caccgcagtg
     3301 cgaccggtgc aatgatcgcg ctggtctgcg gcttcctgct cttccacatc atcgagaaga
     3361 gtattcttat ccatcacatg aatgaaaaag agtatggcca tcatcaccat ccgcatgtgg
     3421 gtgtcgcttc ggcgctagcg ttgagcggac atagcttttt ggacggggta ggtattggac
     3481 tgggcttcca agcgagctcg acggtcggca ttgccgtcgc tatcgccgtt attgcccacg
     3541 attttactga tggcctcaac accgtcaacc tcatgctgct taacaacaat aaatctcgga
     3601 aggccttcat gtttttgctg ctggacgcca cgacgcccat tctcggtgtt ttaagtacca
     3661 agctctttac agtttccggc aatgcactaa cgctgtatct cggctttttc gccggcttct
     3721 tgctgtacat cggcgccagt gaaatcttgc ctgaagccca tagcgagcac tccagctacc
     3781 aaacgattgg tctcaccatt gtcggagtag tctttatttt cattgtcacg cgctttgcct
     3841 agtgtctgtt gctcctcaag aatggtataa ttaaggggcg agtataaaaa tacatacagc
     3901 aaaaatacaa attaatgctc taaa
//
LOCUS       DATNGP010000151         6230 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_3558314, whole genome shotgun sequence.
ACCESSION   DATNGP010000151 DATNGP010000000
VERSION     DATNGP010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6230
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3558314"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..750
                     /locus_tag="VN778_03885"
     CDS             <1..750
                     /locus_tag="VN778_03885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50138.1"
                     /translation="SNSKSIQFKRFKISTGVRRQAGLEATAYNKPVNPTSMFSEAQGN
                     CLGLSLYFSQRVNRNPGWHTIILDDPVQSMDDDHKGNLISLLSELEQTHQVIVFTHDR
                     AFRGSLAEQFKHHPEYLEYEIIKTDKSPVPTIKEQPMRFNQLLNYADICADGPSVQRE
                     SAYTALRKAIENLVVGIASKNKVKLKGADLEQKIKELVGNPVSQTDAGTLDRIRHDCN
                     PNSHDSLAATAPGQIKGFIKDLNKVFANYLP"
     gene            complement(747..1958)
                     /locus_tag="VN778_03890"
     CDS             complement(747..1958)
                     /locus_tag="VN778_03890"
                     /inference="COORDINATES: protein motif:HMM:NF013015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50139.1"
                     /translation="MHIDLNSAFASAEQQAWPVLRGRPMGVTNRLSKECCVIAASYEA
                     KALGIRVGTRMSEALALCPQFIMLETDPTKYTHMYQGLIRIMKDYSPKIKMKSIDEGI
                     IDFHGVHRKQTLEEIGYEIKQRVYKDLGDHMRINVGIGTNRFLAKMAAGWHKPDGLDT
                     LDHRNLIDFYKSIELEDITGIADAFGSRLRAHNIFTPMQFLEADDDLLRRRVFKSING
                     LHWHERLRGYEVDDYETKLGMVGRQWVLPKASNDDEWLRSCLHYLATTAGMKLRYRNV
                     EARGVCVWVSFNMGGGWKDKKMLRQTFYTDADIWLHVSRLFDHRPKHMTIRTMGMYLY
                     ELTPSSKSQLNMFDDVAKAGNLSDAIDEINDFYGNFTVFTADTLTGKKSVKQKIPFGG
                     TEYFELLLKRA"
     gene            complement(1975..2265)
                     /locus_tag="VN778_03895"
     CDS             complement(1975..2265)
                     /locus_tag="VN778_03895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50140.1"
                     /translation="MDPTIEINERVDVVAVYKKQGDISTLCMPCKMRWRNQEINLTEL
                     ALRHPTVAGKRMLHVFHVSDGTNAYRLEFDSEALTWTLVALMAGDYFELSPE"
     gene            complement(2502..2891)
                     /locus_tag="VN778_03900"
     CDS             complement(2502..2891)
                     /locus_tag="VN778_03900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50141.1"
                     /translation="MAVTEKQQQKIWWAVGIAVAIFVVFLWPHDASWDGDGQINAFPS
                     SDSVKNYRLDATMTVSRHWNGWFSKGTYTYTDITGQWPNGGELELDGCEVTDQQQADC
                     TDQDDRTYGIEVVSAPDQPEDDYSSDY"
     gene            2930..3592
                     /locus_tag="VN778_03905"
     CDS             2930..3592
                     /locus_tag="VN778_03905"
                     /inference="COORDINATES: protein motif:HMM:NF014626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HXR50142.1"
                     /translation="MCGRYSFDKVDEFGERFGIDFDPKEIRPNYNVAPTQTMPVIRKV
                     DGKKKVELMRWGIPRFIGPGKVKDVFNTRSDKAFHNWKKMTMTQRILIPATGFYEWQK
                     QAGGKQPFFIHPKDEELFSFAGIWTAWKNEDGKVVNTYSIITTEPNEEMLDIHNRMPV
                     ILHREDEDAWLDGANDNDEETLSEFLMPYEDNGLELYEVSKDVNVTKTNEARLMLPLN
                     SK"
     gene            complement(3585..3806)
                     /locus_tag="VN778_03910"
     CDS             complement(3585..3806)
                     /locus_tag="VN778_03910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50143.1"
                     /translation="MSLIISVEEAREILGSNADSMSDDEIINVIQTLDVLAVEALIKS
                     REQMKADALALAELAYDIYRDKKANEQST"
     gene            complement(3806..4258)
                     /locus_tag="VN778_03915"
     CDS             complement(3806..4258)
                     /locus_tag="VN778_03915"
                     /inference="COORDINATES: protein motif:HMM:NF017470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HXR50144.1"
                     /translation="MSDQQKQSRLNRKSFIFNSLFFLPVIVTGIWLGNAHSDSDFTQG
                     MMGIARLVALVLAGIYIRFTVRRLHDAGRSGWFSFLLIPPATLFCLIYLVIAQKAESN
                     KWGNPTSELRIFGIHARGWRIAGIIIVAILLVYLAGLFATFFFDSGTY"
     gene            4449..4925
                     /locus_tag="VN778_03920"
     CDS             4449..4925
                     /locus_tag="VN778_03920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50145.1"
                     /translation="MGQNNQSDNSLDKKYIEQLIRVENQLEDISYKLDRNAHMMKIVM
                     ESLPQIPVTPILVSELKYIHKTKTLWADERYYINFDGKQADLLARLFTKSGLPKKTHL
                     LVDDLVEESYDHATAEYTKPRTFYLQAKEIQKKLDEGFRTKDLLTVTLKEIYFNHD"
     gene            5064..5603
                     /locus_tag="VN778_03925"
     CDS             5064..5603
                     /locus_tag="VN778_03925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50146.1"
                     /translation="MRKRNEDVRKGLKPLQIKFFALLGQEAPKPIPPKLVQIYGYPLA
                     AIISQLLFWKGMEIRKDGLIYKTEKDFINELGLSSAQQKLAIKKGKAFSFLRVERKGI
                     PAKRHYALNFDLLVEATIREADRKHIVLTKSVFKLVDNDRQRTGGISRTNTDNTTETT
                     ARYNSTASIGDLLPKKYKQ"
     gene            5750..>6230
                     /locus_tag="VN778_03930"
     CDS             5750..>6230
                     /locus_tag="VN778_03930"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HXR50147.1"
                     /translation="MKAYLLARVSTDDQKDALPAQTFRLVEYAKRRGYKYQLAELRES
                     AYKGNRDSFKVVVEEIKACKEPAIVVFDKIDRYTRDSSSEEVRTLQTLYRSGKIELHF
                     PSDNLFIHKDSPATDLLRLGMGIVVAQYYSDAISDNVKRRFEQKLRDGEWIGMAPVGY
                     "
BASE COUNT         1823 a         1409 c         1355 g         1643 t
ORIGIN      
        1 agtaattcca aatcgattca gttcaagcga ttcaaaataa gcactggtgt ccgccgccaa
       61 gcggggctag aggcgactgc ttacaacaag ccggtcaacc caacgtccat gttcagtgaa
      121 gcccaaggta attgtcttgg actaagcttg tacttctcac agcgtgtaaa tcgaaatccc
      181 ggatggcaca ctattattct tgacgaccct gtgcagtcca tggacgacga ccataaaggc
      241 aatcttattt ctcttttgag tgagttggag cagacgcacc aagtcattgt ctttactcac
      301 gaccgagctt ttcgcggcag cttagcggag caattcaagc accacccaga atatctggaa
      361 tatgaaatta ttaagaccga taaaagccct gtacccacca tcaaagagca accgatgcgg
      421 tttaaccagc ttctgaacta tgcggatatc tgtgctgatg gtccgagtgt ccagcgggaa
      481 tcagcctaca ccgcgctgcg taaggccatt gaaaaccttg tggttggcat agcttccaag
      541 aacaaggtca agctcaaagg tgctgacctt gagcaaaaaa ttaaagagct tgtcggtaat
      601 ccagttagtc aaacggacgc cggtactctt gatcggatcc gccacgactg caatccaaac
      661 agtcatgact cgctcgcagc aactgctccg ggtcagataa agggcttcat taaagaccta
      721 aataaagtgt ttgcgaatta tctaccttag gcgcgcttta gaagtagttc gaagtattct
      781 gtgccgccaa agggtatctt ttgcttcacc gactttttgc cggtaagcgt atcggcagta
      841 aacacggtga agttaccata gaaatcgtta atttcatcaa tcgcatcact gaggttacca
      901 gctttcgcaa cgtcatcaaa catattcaac tgacttttgg atgacggtgt tagttcgtac
      961 agatacatgc ccattgtacg tatcgtcatg tgcttcggcc gatggtcgaa caatctagaa
     1021 acatgcagcc atatgtcggc atcagtgtaa aaagtctggc gcaacatttt cttatctttc
     1081 cacccgccgc ccatgttaaa ggatacccat acgcagacgc cccgcgcttc tacgtttcgg
     1141 tatcggagtt tcatgccggc ggtcgtcgct aaataatgta gacacgatct aagccactcg
     1201 tcatcattgc tggccttagg tagtacccat tgtcgtccaa ccataccaag ctttgtctca
     1261 taatcatcaa cttcgtagcc tcgcagtcgc tcgtgccagt gaagaccatt aatgctctta
     1321 aacacacgcc tgcgtagtaa atcatcatca gcttcaagga actgcatcgg cgtgaagata
     1381 ttgtgagcgc gtagccgcga accgaatgcg tcagcgattc cggtgatatc ttcaagttcg
     1441 atactcttgt agaaatcgat aaggttccgg tgatcgagag tgtctagtcc atccggctta
     1501 tgccagccag ccgccatttt agcgagaaag cggttcgtgc ctataccgac attaatgcgc
     1561 atgtggtctc caagatcttt gtaaactcgc tgtttgattt catatccgat ttcctcaagc
     1621 gtctgcttgc ggtggacgcc atgaaagtcg attatgccct catcaatgga cttcattttt
     1681 attttcggtg agtaatcttt cataatccgt attaacccct gatacatatg cgtgtactta
     1741 gttgggtctg tttcgagcat gataaattgt gggcacagag ctagcgcctc agacatacgg
     1801 gtgccgacac gtatgccgag tgctttggct tcataagacg cagctatcac gcagcattct
     1861 tttgatagcc gattcgttac ccccatcggc ctgccgcgta gtaccggcca agcttgctgc
     1921 tcagcagaag cgaaagcact attcaggtcg atgtgcatta cctgtgtctg ctctttattc
     1981 aggcgataat tcaaaataat ctcctgccat caaggccact aatgtccatg tcaaagcctc
     2041 tgaatcgaat tctaaacggt acgcattcgt gccatcgctt acatggaata catgtagcat
     2101 tcgcttaccc gcaactgtgg ggtgtctcaa tgcaagctct gtcagattta tctcctgatt
     2161 acgccaccgc attttgcaag gcatgcaaag tgtactgatg tcaccctgtt ttttataaac
     2221 agctacaaca tcaacacgct cgtttatctc aatggttggg tccatacaac actcacaatc
     2281 tagccttgag tagcggcttg agattagaac ggtaaattgt atgagctgac cgataagcgc
     2341 ttggtagccg tttctactgc aactttgacg ggaagctagg ttgtatggaa gcgcacagag
     2401 cccgtaggat gattgcttgg atttaagtcc gtaaggtaaa ggccatgcca gtcctcacgt
     2461 ggctatacaa ttataaacac acaaagtagc tttatggcaa tctagtaatc gctagagtaa
     2521 tcatcttcag gctggtcagg cgcgctgacc acttcaatcc cataagtacg atcatcttga
     2581 tctgtgcaat cggcttgctg ctgatcggtg acttcgcagc catcaagttc cagttcccca
     2641 ccgttcggcc actgtccggt aatgtcagta taagtataag taccctttga aaaccatcca
     2701 ttccaatgcc gcgaaacggt cattgtggca tctaagcggt agttttttac actatctgag
     2761 gatggaaacg cgttgatctg tccatcacca tcccagctgg cgtcatgagg ccaaagaaac
     2821 actacaaaaa ttgcgacggc gatgccaact gcccaccaga ttttttgttg ttgcttttct
     2881 gtaacagcca tttacacaaa atatacactt aaccatctat aatagaaata tgtgtggacg
     2941 ttattcattc gataaagttg atgagtttgg tgaacgattt ggaatcgatt ttgatccaaa
     3001 ggagattcga ccaaactata atgttgcccc gactcaaacg atgcccgtta ttcgtaaggt
     3061 tgatggcaag aaaaaagtcg agctaatgcg ctggggtatt ccaaggttta tcggcccggg
     3121 caaagtcaaa gacgtattca acaccagatc tgataaagca ttccataact ggaagaagat
     3181 gaccatgact caacgcatac taatccctgc aaccggattt tatgaatggc agaaacaggc
     3241 cggcggaaaa cagccgttct tcattcaccc aaaagacgaa gaactgttct cattcgctgg
     3301 catttggact gcttggaaaa atgaagatgg aaaagtggta aacacctaca gtattatcac
     3361 gactgagccc aacgaagaaa tgctcgatat tcataatcga atgccagtca ttttacatcg
     3421 tgaagatgag gacgcttggc tcgatggggc aaacgacaac gacgaggaaa cattaagcga
     3481 gttcttgatg ccttatgaag acaatggcct tgagctgtat gaagtgagca aggacgtaaa
     3541 cgtaaccaaa accaacgaag ctagacttat gcttccgctg aactctaagt agattgctcg
     3601 tttgctttct tgtctcggta gatatcataa gcaagttcag ctagagctag tgcatccgcc
     3661 ttcatttgtt ctctagattt tataagcgct tcgacagcta agacatcaag agtttggatg
     3721 acatttatga tttcgtcatc ggacatgcta tctgcattac tcccaagaat ctcgcgcgct
     3781 tcttcgacgg aaataattaa actcattagt atgttccact atcaaagaag aacgtcgcaa
     3841 ataatccggc taggtaaacc aaaagtatag cgacgataat gatacctgcg atgcgccatc
     3901 ctcgcgcgtg tatgccgaaa attctcagtt ctgaagtagg attgccccac ttgttagact
     3961 ctgccttttg ggcaatgacg agatagatca aacagaatag tgttgccgga ggaataagta
     4021 aaaaactgaa ccagccgctt cgtccggcat cgtgtaggcg tcgaacggta aatcgtatgt
     4081 aaataccggc gagaaccagc gcgactaatc gggcaatacc catcattcct tgagtgaaat
     4141 cagagtctga atgcgcatta ccaagccaaa tacccgtaac aattacaggt agaaagaata
     4201 atgaattgaa aatgaacgat tttctgttta gtctggattg tttctgctgg tctgacattt
     4261 gggctccctt caagccgaag ggtgccaaat taaatagcga caaccctcta gttgtctaga
     4321 cagcagcaga ttcgcatcca ctgttttcga gagttgccgc atttcaatgc gaatcctctc
     4381 ttagtgagaa attaaaaacc gctgctatct agaccctcat atcataccag agcatctata
     4441 ctaactgtat gggtcaaaac aaccaatctg ataatagttt agataaaaaa tatattgagc
     4501 agcttatcag agttgaaaat caactcgagg acatctctta taagctagac agaaatgcac
     4561 atatgatgaa aattgttatg gaatcgttac cacaaattcc agtgacaccc attttagtgt
     4621 ctgaactgaa atatattcat aaaactaaaa ctttgtgggc cgatgaacgc tattatatta
     4681 atttcgatgg taagcaggct gatctactcg ctcgcctttt tactaaatca ggcttaccaa
     4741 aaaaaacaca cttgctcgtt gatgatttag tcgaggaatc atacgaccat gctacagctg
     4801 aatacactaa acccagaacc ttctacctac aagccaaaga aatacaaaag aaacttgatg
     4861 aaggatttag aactaaagat ttattgaccg ttaccctcaa agaaatttat tttaatcacg
     4921 attaacaggg agtcacagtt ttaaaacgtt ttatcatcca tgaattgctt tactgctatt
     4981 tatgcaggac aagcttggtg caaacgaact caattcatta atcgcgttct ttaagatcct
     5041 cgctgaaata gagaggaata tagatgagaa agcggaatga agatgttcga aaaggcttaa
     5101 agccgctaca aataaaattc tttgccctgc ttggtcaaga agcacccaaa ccaattccgc
     5161 caaagctggt tcagatttat ggctacccgc tggccgctat catttcgcaa ctactgtttt
     5221 ggaaaggtat ggaaatacgg aaggacggat tgatctataa aaccgaaaaa gacttcatca
     5281 acgagcttgg tttatcttca gcgcaacaga agttggctat caaaaagggc aaagctttta
     5341 gcttcctgag agttgaacgt aaaggaatac ctgctaaaag gcactatgcg ctgaattttg
     5401 atttgctggt ggaagcaacg attcgcgagg ctgaccgaaa gcatatagtt ctgactaaaa
     5461 gcgtttttaa gttagtagat aacgaccgac aacgtaccgg cggtattagc aggactaata
     5521 cagataacac tacagaaacc acagcaagat ataacagtac agcatcaatc ggcgatttat
     5581 tgccgaagaa atataaacag tagtaaaatg aaagtgcttt tccagttcga ttcagttacg
     5641 gggattttta tatttctgct cttaatgggc ggttgtgttc agaaaaactc tccgtaagga
     5701 tcgttggact ggaaaagcca caaccgtcca tttttagtaa ggagtagaaa tgaaagctta
     5761 ccttttggca cgagtatcta ccgatgacca aaaagatgcg ctaccggcgc agacttttag
     5821 gcttgtcgag tatgcaaaac gtcggggcta caaatatcag ttagccgaac ttcgagaaag
     5881 tgcctataag ggtaatcgag attctttcaa ggtagtcgtc gaagaaatta aagcgtgcaa
     5941 ggagccggca atcgtagtct tcgataagat agatcgctat acgcgcgatt ccagctcaga
     6001 agaagttcga acacttcaaa ctttatacag gagtggcaag attgaactgc acttcccctc
     6061 cgacaacctc tttattcata aggactcgcc ggcgacagat ttgttaaggc ttggcatggg
     6121 tattgtcgtt gcgcagtatt attctgatgc tatcagcgac aacgtaaagc gtcgcttcga
     6181 gcaaaagctg agagacggag aatggattgg aatggcgccc gtcggttata
//
LOCUS       DATNGP010000152         4705 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_2332448, whole genome shotgun sequence.
ACCESSION   DATNGP010000152 DATNGP010000000
VERSION     DATNGP010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4705
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2332448"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2190)
                     /locus_tag="VN778_03935"
     CDS             complement(<1..2190)
                     /locus_tag="VN778_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008578523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXR50148.1"
                     /translation="MTILLTVLGIILVVAVVAGPIIFLQARRIFREQKNYERGLKFVP
                     LFIHLPPPSDDIDVGSRDVRDVTEETISKAQIIYNIIAGTFEKNFKRNFYGQRHFAFE
                     IVGSQGFVYFYAAVPIALLEIVKQAIVSAYPAARLEEQAEHNIFNPIGKISGTLGGEF
                     VLKENYAYPIATFQDLKRDSMQALLNAMSTLGKEDGVGVQILLRPANPSWRKSASSFA
                     ERKRKGNDKLGASDLAKGVAMAFVKVPESDKEGGSSKKPELSNLQQATYDAIDEKTRH
                     PGYETVIRVVVSSNISQRAQAILHNIIASFSLYDAPGKNGFKFVPAKDVDALATAYIM
                     RFFPQQHSGNILNSVELSTLFHFPDQRNIPTSQLVRQDSKQVDGPRNVPEEGLLLGYN
                     VFRGAKKPIRLGDNDRARHMYVVGQTGTGKSTFLENLALQDMLNGNGFAFVDPHGDTA
                     EKLLAMVPRERTEDIIYFSPADMEYPMGLNLFEFNTPDQKDFLIQEALNMLYKLYDPQ
                     HQGIMGPRYEHLFRNAALTIMADPQGGTFVDIPKLFRDPEYVKQKLQHVTDNTVLEFW
                     QKEMPASQRSNEFGDVVSWFVSKFGAFLSNEMMRNIIGQTTSAFDLRDVMDKKKILLV
                     NLSKGRTGELNSKLLGMVFVMKFQAAAMSRSNIPEEERVDFSLYVDEFQNFSTDSFAT
                     IASEARKYHLNLIVANQFTTQLTQEIRDAVFGNMGTIVSFRIGQNDVE"
     gene            complement(2196..2618)
                     /locus_tag="VN778_03940"
     CDS             complement(2196..2618)
                     /locus_tag="VN778_03940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50149.1"
                     /translation="MNPSSNEGLQLPTPVSNEQLPDSRGAEANDQLVPEQAPVSSPEQ
                     GRSAAQAAVAAIPAMPMPARANDPSATAPTDDSTTTKVATPPMADDSDLIEKEWVSKA
                     KQIVERTREDPYQQSEQLTAVKADYMQKRYNKTIKVDK"
     gene            complement(2652..3023)
                     /locus_tag="VN778_03945"
     CDS             complement(2652..3023)
                     /locus_tag="VN778_03945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50150.1"
                     /translation="MKQILVHHRFGLIILLLAVDSLFFGLTSPQKVASWLLVVGVLLF
                     LTSLYYFLLAGLTVARWYGLPTGKRPRRLAKVLVGTTGFLVALQSIGELSSRDVLVLL
                     PLGVIVYLYSYRAIWRSQPSA"
     gene            complement(3465..3944)
                     /locus_tag="VN778_03950"
     CDS             complement(3465..3944)
                     /locus_tag="VN778_03950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50151.1"
                     /translation="MAEKVFHLQPWTLQVQTVAQKLIGEIRSITPELEVLFMGAAALG
                     LPGKNDIDLDVLCDAREVGRYTRKLRTVLGSPKETNDSMTAWEFQLDGFEVDCMLSDP
                     KISHVPLQRKRFEILKADQKLRDEYKKLKEACDGLPYNEYEKRKAVFLEGKVFSSKS"
     gene            4318..>4705
                     /locus_tag="VN778_03955"
     CDS             4318..>4705
                     /locus_tag="VN778_03955"
                     /inference="COORDINATES: protein motif:HMM:NF024931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HXR50152.1"
                     /translation="MSASVQSIITTGTSGTIVDVECSLSNNLPNIIIVGFANKAVGEA
                     KERIRGAFATSHIVLPRKRTTVNLAPADLPKGDSGFDLAIAVAILLASAQIPDDITDR
                     QAFVGELGLEGEVRPVRGIIGKILVGR"
BASE COUNT         1166 a         1210 c         1086 g         1243 t
ORIGIN      
        1 ttccacgtcg ttttgcccga tacggaaaga tacgatggtg cccatattac cgaagacggc
       61 atcgcggatc tcctgggtca actgcgtagt gaactggttg gccacgatca ggttcaggtg
      121 gtactttcga gcttccgacg caatggtagc aaagctatcc gttgagaagt tctgaaactc
      181 gtcgacatac agactgaagt cgactcgttc ctcttcggga atgttggaac ggctcatcgc
      241 cgccgcctgg aatttcatga caaagaccat acctagcagt ttagagttca gctcgccggt
      301 gcggcccttg ctcaaattca ccaataggat tttcttttta tccatgacat cgcgcaggtc
      361 aaaggcgctc gtcgtttgtc caattatgtt gcgcatcatt tcattactca ggaaggcgcc
      421 gaacttactg acaaaccaac tgaccacgtc gccgaactcg ttggaacgtt ggctggcggg
      481 catttctttc tgccaaaatt caagcacagt gttgtcggta acgtgctgta atttttgctt
      541 cacatattcc ggatcacgga aaagtttagg tatatcgaca aaagttcctc cttgcggatc
      601 agccataatc gtcaatgctg catttctgaa caagtgctca tagcgcggcc ccatgatgcc
      661 ttggtgttgt ggatcataca atttataaag catgttcaag gcttcttgaa tcagaaaatc
      721 tttttggtcg ggcgtgttga actcaaacaa attcaaaccc atcggatact ccatgtcagc
      781 cgggctgaaa tagataatgt cctctgtccg ctcccgcggt accatggcca acagcttctc
      841 ggccgtatca ccgtgcgggt ccacaaaggc gaagccgttg ccgttgagca tgtcttgtag
      901 tgccagattt tctaagaagg tcgatttacc ggtaccagtc tgtccaacta catacatgtg
      961 ccgggcccgg tcgttatcgc cgagacgaat tggcttttta gccccgcgaa agacgttgta
     1021 tcccagcagc aagccctctt cgggcacatt acgcggtccg tcaacctgtt ttgagtcttg
     1081 gcgcaccagc tgcgaggtcg ggatattacg ctgatctgga aaatgaaaca aggtcgacaa
     1141 ttcgaccgag tttagaatat tgccgctgtg ctgctggggg aaaaagcgca tgatgtaagc
     1201 tgtcgccaga gcatcgacat ctttggctgg cacaaattta aagccgttct tacctggcgc
     1261 atcgtagagc gagaaactag ctatgatgtt atggagaatc gcttgcgccc gttggctgat
     1321 attcgacgac accactacac gaataaccgt ttcgtagccg ggatgccgcg ttttttcgtc
     1381 aatcgcgtca taggtagcct gttgcaggtt cgatagctcc ggctttttag agctgccgcc
     1441 ctctttgtcg ctttccggca ccttgacgaa ggccatagcc acacctttgg cgagatcgct
     1501 ggccccgagc ttatcgttac cctttcgctt gcgttcagcg aaggacgaag cgctcttgcg
     1561 ccagctgggg tttgccggcc gcagcaagat ctgtacacct accccgtctt cttttccaag
     1621 ggtcgacatg gcattcaaga gtgcttgcat gctgtcacgc ttgagatcct gaaaggtcgc
     1681 gatagggtac gcataattct cttttaagac aaattcgccg ccgagcgtac cgctgatttt
     1741 accgatagga ttgaaaatgt tgtgttcggc ctgttcttca agacgggcag ccggataagc
     1801 gctaacaatg gcttgcttta caatctccag tagcgcgatc ggcaccgccg cgtagaagta
     1861 tacaaacccc tggctaccaa caatctcaaa agcaaaatga cgctgtccgt agaagttacg
     1921 cttaaagttc ttctcaaaag tgccggcgat aatgttatag ataatttggg ctttgctaat
     1981 ggtttcctct gtcacgtccc gcacgtcgcg gctgccgaca tcaatgtcgt cacttggcgg
     2041 tggtaaatga atgaatagcg ggacgaattt cagcccgcgt tcataatttt tttgctcacg
     2101 aaaaatgcga cgcgcttgca aaaaaatgat tggaccggca actaccgcca ctaccaaaat
     2161 gattcccagt actgttagaa gtattgtcat tttatctact tgtctacttt aatcgtcttg
     2221 ttgtagcgct tctgcatata atcagctttg accgctgtta gttgctcact ttgttgatag
     2281 gggtcttctc tggtgcgctc gacgatctgt ttggctttac tgacccattc tttctcgatc
     2341 aaatcgctat cgtcagccat aggcggtgtc gctacctttg ttgtagtaga atcatcagtt
     2401 ggcgcagttg ctgacggatc attcgcccgc gcgggcatgg gcatagccgg aatcgcggcc
     2461 acggcggcct gcgcggcact gcgcccctgc tccggactac ttaccggggc ttgctcgggt
     2521 accagctgat cattcgcttc agctccgcga gaatcaggca attgctcgtt acttacaggt
     2581 gtcggcaatt gcaaaccttc attagatgag gggttcatgg tatctattat accgcgcacc
     2641 gtacatactt gctacgctga tggctgagac cgccaaatcg cccgataact gtacaggtaa
     2701 acaattaccc ccagtggtaa aagcaccaaa acatcccgcg agcttaactc gccaatcgat
     2761 tgcagggcca ctaagaagcc cgttgtgccc actaaaacct tcgccagacg tctcggtcgc
     2821 ttgccggtcg gcagtccata ccaccgggcc accgttagcc cggccagcaa aaaataatac
     2881 aaagaggtta aaaagagtaa aacacctacg accagtagcc aagaagccac cttctgtggg
     2941 ctcgtaagac cgaaaaacag actgtcaaca gccagtagta agataatcaa tccaaatcta
     3001 tggtgcacga gaatttgttt cattggcgta attcataact ctttttcatt tttcattccc
     3061 aattaaaaag ctgcttcagg catccacctc aagcagccct ttttactatt cttggtgctg
     3121 aggtgacaaa tccgctcgat gtaaaaaact attgtagcgc ttgccacctg cagctgactg
     3181 accgctctag cagcctttgg ggcctttaga gcgttaaacg gatcgctccg catgggcgcc
     3241 ataaacgtgg ccacccgata agtgcgtaag tcgccccctt tgggggcttc ttagcggctt
     3301 cccaatgtaa gtctgggact ccgagttaac caaattgtag gtttttgttt ttgtgactta
     3361 caagaaccaa tctagcacgc cggccccaaa aagtcaatcc tcttatgctt cccctctgga
     3421 taaactttcc gacccacaac atcgtacacg aacctagaat gggattagct tttgcttgag
     3481 aatacttttc cctctagaaa aactgccttc cgtttttcat attcattata tggcagaccg
     3541 tcacacgctt ctttgagctt tttatactca tcacgcaatt tctgatctgc ctttaatatc
     3601 tcaaacctct ttcgctgtaa tggcacatgt gaaatctttg ggtcggaaag catacagtca
     3661 acctcaaatc cgtcgagctg aaactcccat gccgtcatcg agtcattagt ttctttaggg
     3721 cttcctagca cagtgcgtaa ttttcgcgtg tatctgccga cctcacgcgc atcacataaa
     3781 acgtctaaat caatgtcgtt cttgccgggc aggcctagcg cagccgctcc cataaaaagc
     3841 acttcaagtt ctggcgtaat ggagcgtatt tcaccaatca atttttgggc aacagtttgg
     3901 acttgaagcg tccatggttg caagtgaaat actttctctg ccatttgctt taatgataac
     3961 cgaaaacagc agaatctggt ctgagacata ttgattatcc cacccaaaat gctcgaaatt
     4021 tgatggctat tcgaccagat tcgaacatga cacatggaaa gttatgcaca ggttccagaa
     4081 caattaagca taattgttat tgacgtggtt ttgggtattc tataagatac gtatagcgct
     4141 tgaataaatt aagtgctcaa aaacaaaaac gactacgggg gacgacgatt tctcgcaagt
     4201 cgttgtccca ccaggaacaa accaaaactt taaaaaccaa agccaaacaa aaatcttaaa
     4261 ttattcttaa aacaccttgt cttatcccgg gacaaggtgt tttactttgg gcaccctatg
     4321 agcgcttctg ttcaaagcat tatcacgacc gggacttcag gcactatcgt cgacgtcgag
     4381 tgcagcctgt ctaataacct cccgaatatt attatcgtcg gcttcgctaa taaagcagtt
     4441 ggcgaagcca aggagcgtat caggggtgca tttgcgacta gccatatagt gctacctcgc
     4501 aagcggacca ctgttaatct ggccccggca gacctaccaa aaggtgatag cggctttgac
     4561 ctggcaatcg cggtagctat cttgctagcg agcgcacaaa tccctgatga tatcactgac
     4621 cgccaggcat ttgtcggcga gttgggctta gaaggcgaag ttcggcccgt cagaggcata
     4681 atcggcaaaa ttttggttgg ccgcg
//
LOCUS       DATNGP010000153         2510 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_2644930, whole genome shotgun sequence.
ACCESSION   DATNGP010000153 DATNGP010000000
VERSION     DATNGP010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2510
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2644930"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..575
                     /locus_tag="VN778_03960"
     CDS             <1..575
                     /locus_tag="VN778_03960"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HXR50153.1"
                     /translation="KSDISDFFRVNGYPPETVEFKQLAQSDFKDWRLSVGGLVEHRLD
                     LSLDDIKALPRREQITKHICIQGWSAIGEWGGAPMTEILARCKPDKRAKYVVFHAYDA
                     DGDGKPYYEVLRTSDMYDQMSILAYEMNWKPLNLEHGAPLRLRCERKLGYKMVKYIKS
                     IEFIESFKQIGDGRGGYREDNVMFDWEASI"
     gene            812..1525
                     /locus_tag="VN778_03965"
     CDS             812..1525
                     /locus_tag="VN778_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018158873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50154.1"
                     /translation="MKKLLLLPVIAATILTLSLQTVSASSFYASGSTGIDVSYPNCTA
                     KVPGVSFGIVGVTGGTVFSDNSCLALEAKNFTDLSLYVNTGLNASKSSAYYAQAQVGC
                     NGDVYCAAYNYGYNAAKDAAAYASAQGVSSAKWWLDVETSNTWSKDVLQNKKSLQGEY
                     DALVANGAVLVGVYSTTAQWQSITGSWQNNWASWGATTWTSASGAKTYCTGHQFTGGP
                     SLLMQYKSRQSKIDQDVAC"
     gene            complement(1609..>2510)
                     /locus_tag="VN778_03970"
     CDS             complement(1609..>2510)
                     /locus_tag="VN778_03970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50155.1"
                     /translation="DMSDGIHVYFDDVTDPSHVQNADTFNETDIATLSYTATHNIKFS
                     MDFFNGPDNDVVRVYIDGNLAVTGTSWEDYYRFDQESNPGLTHNDSRVVDTLLFREGG
                     SAVPSNAGNGYLVDNLSMLSGDFASGLPTTKDMCMKNGWQNYGSAFKNQGDCVSFIAS
                     KGKNRPTGALPQASVTHSAIGNLALSGPEQTLSFHAYDNGISANDSGWISYANPGVGL
                     SYTVPPTCVNVSGSTAYFSYQIPADAPVAANTWVVWKVTDGSTDTAGFTTASDGNSAR
                     ALCEAGSSAVANYTITSGDVVVQ"
BASE COUNT          642 a          652 c          668 g          548 t
ORIGIN      
        1 agaagtccga tatctcagac tttttccggg tcaatggcta cccgccggaa actgtagaat
       61 tcaagcagct ggctcagagt gatttcaaag attggcggct aagtgtcggc ggcttggtag
      121 agcaccggct ggatttatca ctcgatgaca ttaaggcact accgcgacgc gaacaaatta
      181 ccaagcacat ttgtatccag ggctggagcg cgattggcga atggggcggc gcgccgatga
      241 ccgaaatcct agcccgctgc aaacccgaca aacgggcaaa atatgtcgta tttcacgcct
      301 acgacgccga tggcgacggc aagccctact acgaagtact tcggaccagc gatatgtacg
      361 accaaatgag tattttggcg tacgaaatga actggaagcc gctcaacttg gaacatggtg
      421 cgccgctccg cctgcgctgc gaacgcaagc tgggctacaa aatggtgaag tacataaaat
      481 ccatcgagtt cattgaaagc ttcaagcaga ttggcgacgg ccgcggcggt taccgcgagg
      541 acaacgtcat gttcgactgg gaagcatcta tataatagtg tccatgagcg agagactctg
      601 gagcggaaag tacggagcac tcgccggcgc ttatttcgcg aaggactgcc gtaccaaaaa
      661 ccgcagctaa ctcaagagcg aacgctgaaa accggtggcg tcatcgtgag atatcaactc
      721 atcagctcat agaacgcgcc ggcaagcacg cgcatcagag aattaaggta gaatagatat
      781 agtttcaagc taaccaggaa ataaaatatt catgaaaaaa ctgctactac tccccgttat
      841 cgctgcaacc atcctaacac tctcgctgca aaccgtatcc gcctcaagct tttatgccag
      901 cggctctaca ggcatcgatg tcagttatcc aaactgcacc gccaaagtac ctggcgtcag
      961 tttcggtatc gtcggcgtga ccggcggtac ggtgtttagc gataacagct gtttggcatt
     1021 agaagcaaag aattttactg atctaagcct gtacgtaaat accgggctga acgccagcaa
     1081 gagctctgcc tactacgccc aagcacaagt gggctgtaac ggcgacgttt actgcgcggc
     1141 ctacaactac ggctataatg cagcaaagga tgctgctgcc tatgcgagcg cgcaaggcgt
     1201 tagcagcgcg aagtggtggc tcgatgtcga gacatctaac acctggagca aggacgttct
     1261 gcagaacaag aagagtttgc aaggcgagta cgatgcattg gtcgcgaacg gtgcggtgtt
     1321 agtaggcgtc tacagcacta ccgcgcagtg gcagagcatt accggcagct ggcagaacaa
     1381 ttgggctagc tggggcgcca caacctggac gagcgcatcg ggagccaaaa cttactgcac
     1441 ggggcaccag tttaccggcg gcccgagctt gctcatgcag tacaagagcc ggcaaagcaa
     1501 gatcgaccaa gacgtcgcct gctaagatct ccggcactct atattcctca cgagactaaa
     1561 ataccccagc attacgaagg ggtattttag tctagctttc gccttttact attggactac
     1621 aacgtcgccc gaggtaatcg tatagttggc cactgcagag ctacctgctt cacagagcgc
     1681 acgagcgcta ttaccgtcgg aggccgttgt gaagcctgcc gtatcggtgg aaccatctgt
     1741 taccttccag acaacccatg tgttcgccgc tacaggcgca tccgccggaa tttggtacga
     1801 gaagtatgcc gtgctaccgc tcacgttcac gcaggttggc ggaacggtat aggaaaggcc
     1861 tactccgggg ttagcgtagc tgatccagcc actatcgttg gccgagatgc cgttgtcata
     1921 ggcgtggaag ctgagcgtct gctccgggcc cgacagtgcc aggttgccaa tcgcagaatg
     1981 agtcacacta gcttgaggca aagcaccagt tggccgattt ttacctttcg acgctatgaa
     2041 gctgacgcag tcgccctgat tcttgaatgc cgaaccgtag ttttgccaac cattcttcat
     2101 gcacatgtct tttgtcgttg gcagcccgga tgcgaagtca ccggaaagca tgctcaaatt
     2161 atcaaccaga taaccattgc ctgcattgct tggtactgcc gaaccgcctt cgcggaacag
     2221 cagagtgtca actacgcgac tatcgttatg ggtaaggccg gggtttgatt cttggtcgaa
     2281 gcgatagtaa tcctcccaac ttgtgcccgt cacagcaagg ttgccatcga tataaactcg
     2341 gaccacgtcg ttgtcaggcc cattgaaaaa gtccattgag aattttatgt tatgggttgc
     2401 agtatagcta agggtagcga tatcagtctc gttgaatgta tcagcatttt gaacatggct
     2461 tgggtcagtc acgtcgtcga agtagacgtg gatgccatca ctcatatcga
//
LOCUS       DATNGP010000154         5279 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_1402357, whole genome shotgun sequence.
ACCESSION   DATNGP010000154 DATNGP010000000
VERSION     DATNGP010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5279
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1402357"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1395
                     /gene="dnaX"
                     /locus_tag="VN778_03975"
     CDS             <1..1395
                     /gene="dnaX"
                     /locus_tag="VN778_03975"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR02397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HXR50156.1"
                     /translation="TQTLDQALKQGRISHAYLLTGPQGVGKTSVARILAHEINSLPYT
                     DNSPHLDIIEIDAASNRRIDEIRDLRDKVYVAPTSAKYKVYIIDEVHMLTREAFNALL
                     KTLEEPPEHVVFILATTDAHKLPETIVSRTQRFTFKPVEKGEVIDHLRAIAKEEKISV
                     SDEALDLLAEHGEGSFRDSISLLDQASSQGSKLDASAVQALLGIPPDEGIQQLLQQIA
                     QPSAGLASVAEALHQLYAQGYQPAVIAKRLGQLLREQLLSNQTALSSRETTTLLAQLL
                     EVPASVDPQAFLEIILLAAASNMTGAFNSDAPVQQPKALKDAPAEPEKQIAKPADKSK
                     AVEQPKPKPAKRSSVNNGIWPEVLQALKQSHNTLYGIVRMAEPTFGEDGTLTLAFAFA
                     FHQKRLNDTKHRQLLAGVIEEISGQTVQIECIVDKTVSSTPAAEVPLEQARPEGNGAL
                     STISNIFGGGELLE"
     gene            1421..2782
                     /gene="dnaB"
                     /locus_tag="VN778_03980"
     CDS             1421..2782
                     /gene="dnaB"
                     /locus_tag="VN778_03980"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008578878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HXR50157.1"
                     /translation="MEPPQATQPPQNVEAETSLLGSLLIDSDAIVKIADIVSASDFFE
                     PKNARIYEAIMQLYDRREAIDVLTLADRLRSNGYLDNVGGAAYLTELTNYVPTATHVE
                     QYADIVAQKALRRRLIVASKELVGLGYDESKQLSELIEEAETRLFEVSQQHVKQSVVS
                     LEAILAESFDRLDDLHKDKKKIRGIATGFRDIDDKLAGFQRSDLIVLAARPSMGKTAL
                     ALNFAHKVAVQANQPVLVFSLEMSKEQLVDRLLSMESGVDAWALRTGNLTDADFEKIA
                     RAMGTLSEAQIFIDDTPGITVSDLRTKARREAHQRPLGLIIVDYLQLMSGGSRFGGES
                     NRVQEISEISRGLKGVARELNVPVLALSQLSRSVENRHPQIPQLADLRESGSIEQDAD
                     VVMFIYREEYYNPETDRKNLSDIFIKKHRNGPTGGVELRFESEKQRFSSVDSRHAADP
                     FME"
     gene            2826..4013
                     /locus_tag="VN778_03985"
     CDS             2826..4013
                     /locus_tag="VN778_03985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50158.1"
                     /translation="MREMFTYIKANAQQVVLSAAGIVLLFGLLLYHLGSLTIGLSAVE
                     QQTATTPLGWHGLYHHPFYLPLTFVRSIVFFLFAHHGQTLTRLPNILFGALSILAFAA
                     LIKLWHSTRTAFLATLLFATNAWVLHVSRLASYDVLYLWAMPVLLLIYMAMQKGYERP
                     VVFYGSALLLGLLLYIPGLVWLVILEVFWLRSELLEGWKYFDRVWERILYGLSYIIWL
                     PLLVWRLHNINNLRMWLGLPAHLATPFTLLKQLVAVGVHLFIRGPQYPQVWLGRTPML
                     DLFTLAVCLLGIYFYVTHLSTTRSRVLISWFVVGAVLVGLGGPVSLSLMVPLVYIAVA
                     TGIAYLLHRWLHVFPLNPLARGFGIGLIVVAVAVSSVYNLRAYFIAWPHNPTTQATFH
                     YRR"
     gene            4057..4350
                     /locus_tag="VN778_03990"
     CDS             4057..4350
                     /locus_tag="VN778_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015642082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HXR50159.1"
                     /translation="MSRFDQAKMLMRVRKIQKELQNQIITVEKGDGAVKVEITGEQKL
                     KKVHINPKSVDLDDIGQLERWVEDAVKEAIAESQRIAAEKMQPMMGALGNLGM"
     gene            4363..4968
                     /gene="recR"
                     /locus_tag="VN778_03995"
     CDS             4363..4968
                     /gene="recR"
                     /locus_tag="VN778_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010165493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HXR50160.1"
                     /translation="MQVLPPALTDLIDAFGLLPGVGPRTAERYAYYLVKHDGDAPHKL
                     AAALDVLTDSIGYCKKTFALVPAGQDLSDLYTDPRRDKKTIAVVAEPFDLVALEKTGQ
                     FHGTYHVLGGLVSPIDNVSPEQLRIKELVRRIDEDNVEEIILATNASVEGESTALYIQ
                     QQIGDRQVKITRLARGLPIGVDLEYADQITLGRALEGRQAL"
     gene            4965..>5279
                     /locus_tag="VN778_04000"
     CDS             4965..>5279
                     /locus_tag="VN778_04000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50161.1"
                     /translation="MTTYAEAEEIKHIIEAAQNIVIVQADNPDGDSLGSALALEHMLG
                     DLGKEPYLYCGVDMPTYLRYLSGWDRIQRELPNQFDASIIVDASTMTLFEKLANSGQQ
                     GWL"
BASE COUNT         1299 a         1347 c         1362 g         1271 t
ORIGIN      
        1 actcagacgc tagatcaagc gctcaagcag gggcgaatta gtcacgccta tcttttgact
       61 ggaccgcaag gtgtgggcaa gacttcagtg gctcggattc tcgctcacga aattaatagt
      121 ctgccatata ccgataacag cccccatctg gacatcattg aaattgacgc agccagcaac
      181 cgacgtatcg atgagatccg tgatttacgc gataaggtgt acgtcgcgcc caccagtgct
      241 aaatataagg tatacatcat tgatgaggtc cacatgctga cgcgagaggc attcaatgcc
      301 cttctgaaaa ccctcgagga acctcctgaa catgttgtct ttatcctagc caccactgat
      361 gcccataagc tgcccgaaac catcgtcagc cgtactcagc gcttcacctt caagccggtt
      421 gagaaaggcg aagtaattga ccacctgcgg gcgattgcta aggaagaaaa aatttctgtc
      481 agcgatgaag ctcttgattt actggcggaa cacggggaag gaagctttcg cgacagcata
      541 agcttgctcg accaagccag cagtcagggc agcaagctag acgcttcagc ggtgcaagcc
      601 ctccttggaa taccgcccga tgaagggatt cagcagttat tacagcagat cgctcagcct
      661 tcggcaggtc tcgcgagcgt tgcagaggcc ctgcaccagc tctatgccca ggggtaccaa
      721 ccggcagtca tcgccaagcg gctgggtcaa ctacttcgtg agcagttgtt gtctaatcag
      781 acagcgttgt cgtctaggga gacaacaacg ttattggctc aactcttaga ggtaccggct
      841 tctgtcgatc cgcaagcgtt cttggaaatc attcttttgg cagctgcgtc gaacatgact
      901 ggtgcgttta atagtgacgc tcccgttcaa cagccgaagg ccttaaaaga cgccccggca
      961 gagcctgaaa agcaaatagc taagcctgct gacaaatcga aggcagtaga acaacccaag
     1021 cccaagccgg ctaaacgcag ctcggtcaat aatggtattt ggccagaggt tttgcaggcg
     1081 ctcaagcaga gccataacac cttatatggt attgtgcgta tggctgagcc aacattcggc
     1141 gaggacggta ccttgaccct ggctttcgct ttcgcttttc atcaaaagcg cctaaacgat
     1201 accaaacacc gccagcttct ggcaggcgtc attgaagaaa tcagcggtca aacggtacaa
     1261 attgaatgca tcgtcgacaa aacggtgagc agcacgccgg cagctgaagt gcccctagaa
     1321 caagcgaggc ctgaaggcaa cggcgcgctt tcaaccatta gcaatatttt tggcggcggt
     1381 gagctgctag aataacccat aagggagtga tcgcaggata atggaaccgc cacaggctac
     1441 tcagccacct cagaatgttg aagccgaaac ctcgttgctt ggctcattgc taattgatag
     1501 cgatgccatt gtcaaaattg ccgatattgt gagcgccagc gatttctttg agcccaaaaa
     1561 cgctcgtatt tatgaagcga ttatgcagct atacgatcgg cgtgaagcta tcgacgtact
     1621 caccctggcc gatcgtttac gaagcaacgg ctatttggat aatgtcggcg gggcggccta
     1681 tcttaccgag ctcaccaact atgtaccaac tgctacccac gttgagcagt atgccgatat
     1741 tgtggctcaa aaagcgctgc gcagacgcct gatcgtcgct tccaaggagc tcgttggcct
     1801 ggggtatgat gagtccaagc agctgagtga gctgattgaa gaagccgaaa ccagactatt
     1861 tgaggtgagc cagcagcacg tcaaacaaag cgtggtcagc ctggaagcta ttctggcgga
     1921 aagttttgac cggctagacg acctgcacaa ggataagaaa aaaatccgtg gcatagcgac
     1981 tggttttagg gacatcgatg acaaactagc tggctttcaa cgctcggatt tgattgtact
     2041 cgcggcccgt ccttcgatgg gcaaaaccgc tcttgctttg aacttcgccc acaaggtagc
     2101 cgtccaagct aaccagccgg tgcttgtctt cagcttggaa atgagcaagg agcagctcgt
     2161 ggaccgcttg ctttccatgg aatccggcgt cgatgcctgg gcgctgagga caggaaatct
     2221 gaccgatgcc gattttgaga agattgctcg agctatgggc acgctgagcg aggcccagat
     2281 tttcatcgat gacacgcctg gtatcaccgt cagtgacttg cgcaccaaag cccggcgcga
     2341 agcccaccag cggccgctcg gtttgatcat cgttgattat ctgcagctga tgagcggtgg
     2401 cagccgtttt ggtggcgagt ccaatcgggt acaagagatt tcagagattt ctcgtggcct
     2461 caaaggtgta gcgcgggaac tcaatgtgcc cgttctggcc ctcagccagc tcagtcgctc
     2521 agttgaaaac cgccaccctc aaataccgca gctggccgac ttacgagagt ccggctcaat
     2581 cgagcaagat gccgacgtgg tgatgtttat ttatcgggaa gaatactaca accctgaaac
     2641 ggatcgtaaa aacctttccg atatatttat caaaaaacac cgtaacggcc ctacgggggg
     2701 cgtggagctt cgctttgaaa gcgaaaaaca gcgcttcagc tctgttgatt cgcggcatgc
     2761 agccgatcca tttatggaat aatgcactgc aaataacgcc cggcgagcta taataaacga
     2821 taagcatgag ggagatgttt acctacataa aagccaatgc tcagcaagtc gtgctgtcgg
     2881 ccgccgggat cgtgctgctt ttcggcctgt tgctgtacca ccttggttca ctaaccatag
     2941 gcttaagtgc tgtcgagcaa caaacagcga ccacaccact tggttggcat ggtctgtacc
     3001 accacccgtt ttatttgccg ctcacttttg ttcgctcgat agtatttttc ctgttcgcac
     3061 atcatggtca aaccctaacc aggctaccga atattctctt cggcgcctta tcgatacttg
     3121 ccttcgcagc acttattaag ctttggcaca gtacgcgcac cgccttcttg gcgactctgt
     3181 tgtttgccac caacgcctgg gtactgcacg tcagtcgttt agctagctac gacgtacttt
     3241 atttatgggc catgccggtc ctgctgctaa tttatatggc catgcaaaaa ggttatgagc
     3301 ggccagttgt tttctatgga agtgcgctcc tactgggcct tttgctttac attccaggtc
     3361 ttgtttggct cgttatactc gaagtattct ggctgagaag cgagctcctg gaaggctgga
     3421 aatacttcga tcgtgtttgg gagcgcatat tgtacggctt aagctatatt atttggttgc
     3481 cgcttttggt atggaggctg cacaatatca ataatctccg catgtggctt ggcttgccgg
     3541 ctcatttggc aactcctttc acactcttaa agcaacttgt ggccgtaggc gtgcacttat
     3601 ttattcgcgg cccacagtat ccgcaggttt ggttaggtag aacgcccatg ctagatttat
     3661 ttacgctcgc ggtttgtttg ctcggcattt atttttatgt aacgcacttg tcgactaccc
     3721 gtagccgggt gcttatcagt tggttcgtgg tcggagccgt attggttggc ttaggtggcc
     3781 cggtcagttt gagtttgatg gtaccactgg tatatatagc tgttgccacg ggtatcgcct
     3841 accttttgca tcgctggtta catgtttttc cgctcaaccc cctagctcga ggtttcggca
     3901 tcgggctaat tgttgtagcc gtcgctgtaa gctcagttta taatttgcga gcctatttca
     3961 ttgcctggcc gcataatccc actacccaag cgacttttca ctatcgtcgt taagctatat
     4021 actaaagaga ttgaagcagg gaggaatacg aaagccatga gtcgattcga tcaggccaaa
     4081 atgctgatgc gtgtcagaaa aatccaaaaa gaactacaaa accagatcat caccgttgaa
     4141 aaaggcgacg gcgcagtcaa ggtggagatt actggcgagc aaaagctaaa gaaggtacac
     4201 atcaacccta aatctgtcga tctcgatgac attggacaac tcgagcggtg ggtagaagac
     4261 gccgtcaaag aagcgatcgc tgagagccag cgcattgcag ccgagaagat gcagccgatg
     4321 atgggcgctc taggtaatct gggcatgtaa gtatactcga gcatgcaagt tctaccgccc
     4381 gcgctgactg atctgattga cgcctttggc ttactgccag gtgttggtcc gcgcacggct
     4441 gagcgctacg cgtattatct cgtcaagcat gacggtgatg ctcctcacaa gctagcagct
     4501 gctctcgatg tcttaaccga tagcatcggc tactgcaaaa agaccttcgc gctggtgccg
     4561 gctgggcaag acctttcgga tctctatacc gatccacggc gcgataaaaa aactatcgct
     4621 gtagtggctg agccctttga ccttgtggcc ctagaaaaaa ctggccagtt tcatggcacc
     4681 taccatgttt tgggggggct cgtctcacca atcgacaatg ttagccctga gcagctacgc
     4741 atcaaagagc tagtccggcg gatagatgaa gacaacgttg aagagattat tttagctact
     4801 aatgccagcg tggaaggcga atcgacagcc ctctacatac agcagcaaat tggtgaccgc
     4861 caggtgaaaa tcacgcgctt ggcccgcggg ttaccaatcg gcgtcgatct agagtacgcc
     4921 gatcaaatca ccttgggccg cgcgctagaa ggcagacaag ctttatgact acgtatgccg
     4981 aagctgagga aattaagcac attatcgagg ctgcccaaaa catagttatc gtgcaggccg
     5041 acaatcctga tggcgattct ttaggtagtg cgctggctct ggaacatatg cttggggatt
     5101 taggcaaaga gccatatctg tactgtgggg tggatatgcc aacctatctt cgctacttat
     5161 caggctggga ccgtattcag cgggaactac ctaatcagtt tgacgccagc attatcgtgg
     5221 atgcctcgac gatgaccttg tttgaaaagc tggccaactc cgggcaacaa ggctggctg
//
LOCUS       DATNGP010000155         2386 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_2585060, whole genome shotgun sequence.
ACCESSION   DATNGP010000155 DATNGP010000000
VERSION     DATNGP010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2386
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2585060"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..283)
                     /locus_tag="VN778_04005"
     CDS             complement(<1..283)
                     /locus_tag="VN778_04005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50162.1"
                     /translation="MDKTASSRTAPTSKPSAYPRSYIIGLIVGVVLFVPSLAIAHMHE
                     LKGLQLSLFRDLNNLSNSFRVPALWITEGLGAAYPIAACVVLPALFKRFR"
     gene            408..947
                     /locus_tag="VN778_04010"
     CDS             408..947
                     /locus_tag="VN778_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010795549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HXR50163.1"
                     /translation="MADLKGKKVAILVDDYFEEAEFTGPLNDLKKAGAEVEVIAPKAG
                     EIHGMNHVEIADTFRADKALDDVKIDEYDALVLPGGAVNADQLRMNDKARIWIKQFMN
                     SGKPVAAICHAPWALASAGVAKGRRLTSFFTIQDDMRNAGANWVNEEVVIDSNLITSR
                     KPDDVPAFDNALIEMLQAA"
     gene            985..1656
                     /locus_tag="VN778_04015"
     CDS             985..1656
                     /locus_tag="VN778_04015"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF013356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HXR50164.1"
                     /translation="MRFILATGWEDGVAGLTQRLVSALAEGKRVLWLVSGGSNIDAAV
                     DVMDTITPRLSQGLSILLADERFGQEGHSDSNWTNLMKAGFKPKRALLLPVLCGKNTL
                     EEALAHYRDIVRKAYAEHDVIVVQLGMGVDGHIAGILPNSAAAKEKKELVVSYKSNPY
                     TRLTLTFPALKRAHHAYVFAFGDTKAQALFDLHNKRLSLLKQPAQILKRLQDVSVYND
                     QLGDD"
     gene            1680..>2386
                     /locus_tag="VN778_04020"
     CDS             1680..>2386
                     /locus_tag="VN778_04020"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HXR50165.1"
                     /translation="MKIAIHGLGRMGMQIARKLSENGHTVIAHNRSPEPVAEAVTYGA
                     IGTKTKEEVIEHFGGDTVVLWIMLPADIVDAHVEEWLAQMPEGSIIVDGGNSDYRLTQ
                     KLNDTIKARGCHLIDVGTSGGVWGYQNGFSMMAGSDEASAFQALEPALQTLAKPEGAY
                     HLFGPSGSGHYVKMVHNAIEYGMMESLAEGYRLLKEGPYKELDLAAAGDVWQHRSVVT
                     SWLNELTRDALAENPQL"
BASE COUNT          630 a          576 c          659 g          521 t
ORIGIN      
        1 agcggaagcg tttgaataaa gccggcagca ccacacacgc ggcaatgggg taggcggcgc
       61 cgaggccttc tgtaatccac aatgctggta cccggaacga gtttgacaag ttattgagat
      121 cgcgaaacaa gctcagctgc agacccttta gctcatgcat atgcgcaatc gcgagcgacg
      181 ggacgaacag cacgacacca acgatcagcc cgataatata tgaccgcgga taggcggatg
      241 gtttactggt cggtgccgta cgagagctcg ctgttttgtc catcgttaag ctcaagtata
      301 agcgaaaagg gctgctaagg gcaattttac ggacaggtaa caattctcac agctcgcggg
      361 tcgttagcta gggcataatt acctctgtta atttagtagg agggcatatg gctgacttga
      421 aaggtaaaaa ggtagcaatt ctagtagatg attatttcga ggaggccgaa tttaccgggc
      481 cattgaatga tcttaaaaag gccggagctg aggtagaggt gattgcaccg aaagccggtg
      541 aaatacatgg catgaatcac gtcgaaatag cggacacgtt ccgagctgat aaagcgctcg
      601 acgatgtcaa aattgacgag tatgacgccc tggtacttcc ggggggagca gtaaacgccg
      661 atcagttgcg catgaatgac aaggccagaa tctggatcaa gcagtttatg aattccggca
      721 agccagtagc agcaatttgt catgcaccct gggctctggc ctcagccggt gtcgccaagg
      781 gacggcgact gaccagtttc ttcaccatcc aggatgatat gcggaatgcc ggagctaatt
      841 gggtgaacga agaagtggtt atcgattcta atctgatcac cagccgcaaa ccagatgatg
      901 taccggcctt cgataacgca ctgattgaaa tgctgcaagc cgcttaggca ataacacttt
      961 tgctctatct gatacaatga aagcatgcgc tttatcctgg ccaccggttg ggaagatgga
     1021 gtggccggat taactcagcg gcttgtctca gcactggccg agggtaagcg agtactttgg
     1081 ctagtcagcg gcggctcaaa tatcgatgcc gccgtagatg ttatggacac tatcacacct
     1141 cgcctcagcc aggggctcag cattctctta gcagacgagc gctttgggca ggaagggcat
     1201 agcgactcta actggacgaa ccttatgaaa gccgggttta aaccaaaaag ggccttactc
     1261 ctgccggtgc tttgcgggaa aaatacactc gaggaagcac tggcgcatta ccgtgacatt
     1321 gtccggaaag cttatgccga gcacgatgtg attgttgttc aattaggcat gggcgttgac
     1381 ggacatatcg ccggcatctt accgaattca gcggccgcca aggagaaaaa agaacttgtt
     1441 gtgagttata aaagtaaccc atacacgcgc ttgacgctca cttttccggc gctcaagcgg
     1501 gctcatcatg cgtatgtctt cgctttcggt gataccaagg ctcaagccct ctttgatctg
     1561 cataataaaa gattgtctct tctcaagcag ccagctcaga ttcttaagcg cctgcaggac
     1621 gtgagcgttt ataatgatca attaggcgac gactgaaata tggctgaaag gagcatttca
     1681 tgaaaatagc gatacatggc ttgggccgca tgggtatgca gattgcccga aaactgagcg
     1741 aaaacggaca tacggtgata gcccacaacc gcagcccaga accagtagct gaagctgtca
     1801 catatggggc gatcgggact aagaccaaag aagaagttat tgagcatttc ggtggggata
     1861 ctgttgtcct ctggatcatg ctgccggcgg acattgttga tgcccatgtg gaagaatggc
     1921 tagcgcaaat gccagagggt agtattatcg tagacggcgg caattccgat taccgactga
     1981 ctcagaagct aaacgatacc atcaaagcca ggggctgcca tctgatcgat gtcggcacca
     2041 gcgggggcgt ctgggggtac caaaacggct tttcgatgat ggctggcagc gacgaggcat
     2101 cggcttttca ggccttggaa ccagctcttc agacacttgc gaagcccgag ggcgcctacc
     2161 atctttttgg cccaagcggc agcggccact acgtaaaaat ggtccataat gccatcgagt
     2221 acggcatgat ggagtcgcta gccgaaggtt atcgcctgct aaaagaaggt ccctacaaag
     2281 aacttgacct ggccgccgcc ggtgacgtct ggcaacaccg tagtgtggtc acctcttggc
     2341 tcaacgagct gacacgagat gctctcgccg aaaatcctca gctcga
//
LOCUS       DATNGP010000156         2768 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_3566155, whole genome shotgun sequence.
ACCESSION   DATNGP010000156 DATNGP010000000
VERSION     DATNGP010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2768
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3566155"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..351)
                     /locus_tag="VN778_04025"
     CDS             complement(<1..351)
                     /locus_tag="VN778_04025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50166.1"
                     /translation="MKQRLILLLSLVCLVVSCLGPSSAQAATQPHGLAITPLRQYLKA
                     VAGTTTNSSLTISNLTDKPLDVALSVRQFSLTDYTYNYQFTQPKNDWLRIHTPTVALQ
                     PQQTKAIGYELNIPP"
     gene            complement(370..969)
                     /locus_tag="VN778_04030"
     CDS             complement(370..969)
                     /locus_tag="VN778_04030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50167.1"
                     /translation="MPTKTTPKSKKPLKLSLACGDNKPEGFTGVDIAKTNDTDFVQDL
                     LVFPWTQFKDASVDEIECSHFVEHIPHGDGYHDPFFQFFDEIYRILKPAEFDPANPNI
                     ATSGFARIICPYYSSMRAWQDPTHQRAISEASFLYLNKQWRIDNKLDHYPVSCDFDFT
                     YGYVLTQEWQTRSQDAQTFAIQHYKNVVSDIQVILTKRS"
     gene            complement(979..1704)
                     /locus_tag="VN778_04035"
     CDS             complement(979..1704)
                     /locus_tag="VN778_04035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50168.1"
                     /translation="MGQPQILVPDNAGIHNASDYATRLEREKTYRDLSTIIVCPTRGM
                     IPARIVQSWQGLIRPMNQKALGPLFMEGMEVGEAYNQVIKMILENDELSKFKFLLTIE
                     EDNAPPADGLLKLYESIDEYDAVGGLYWTKGEGGQPMCYGDPAAMPLNFIPRIPQPDT
                     ITPCNGLGMGFTLFRMEMLRDKRFDYGNWFKTKQEVLPGGGAQVFTQDLWFFNHAREF
                     GYKFACDSRVKVGHYDAANQLMW"
     gene            complement(1721..2392)
                     /locus_tag="VN778_04040"
     CDS             complement(1721..2392)
                     /locus_tag="VN778_04040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50169.1"
                     /translation="MAIAFRNESHNINNGGSPTGTEPTGTVQGDTLIALCEMENNGTL
                     TPPAGWTTIFSGTSATAQFNYCLSYIIRGASAPNLTWTFGASGYYELYIVGFSGCDTS
                     NPIDASAKTPDASGTSINPDPPLLTAISSSAMALAICDNWNGSDTGGWTTPSGYTIVT
                     NNATGNDCAIATKLLTSSGPENPSAFGNVGSAGGGSTATDYWAATITLSPPRVAATAI
                     AWIRA"
     gene            complement(2399..>2768)
                     /locus_tag="VN778_04045"
     CDS             complement(2399..>2768)
                     /locus_tag="VN778_04045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50170.1"
                     /translation="SSVSSTSLTSVSGMRQLTVPMNVNMTPGEYWIAAVMSSATTAPG
                     ATMTLYGNNLFTPTSGAAVLTPIGSGTTAARDVVLFEGVYTAATSAGPATISGAQINN
                     TAASVLQSANFYNAIFNATY"
BASE COUNT          625 a          629 c          805 g          709 t
ORIGIN      
        1 ggggggtatg ttcagttcgt acccaatagc tttggtttgt tgcggctgta gtgccacagt
       61 aggcgtatgg atacgcagcc aatcgttttt tggctgagtg aattggtaat tgtatgtgta
      121 gtcggtaagt gagaattgcc gaaccgatag ggcgacgtcc aatggtttgt cggttaggtt
      181 gctgatcgtc aaggaactat ttgtagtggt gccagctaca gccttaagat actgcctcag
      241 cggtgtgatc gctaggccat gtggctgggt ggctgcttga gcgctgcttg gccccagaca
      301 acttaccacc aggcaaacta gcgacagcag cagaataagc cgttgcttca aacttctggc
      361 cattgttctt taagaacgct tcgttaggat aacttggata tcactgacta cgtttttgta
      421 atgttggatg gcaaatgtct gagcgtcttg gctgcgggtt tgccactcct gggttaagac
      481 atagccatag gtgaaatcaa agtcgcagct aacagggtag tgatctagct tattatcaat
      541 ccgccattgt ttgtttaggt acaaaaaact ggcctcggaa atagcccgct ggtgggttgg
      601 gtcctgccag gcacgcatac tggagtagta cgggcagata atgcgtgcaa agccggaggt
      661 ggcgatatta ggattggcag ggtcgaattc tgccggcttt aggatgcggt agatctcgtc
      721 aaagaactga aaaaatgggt cgtggtagcc gtcgccgtgc ggaatatgct cgacaaagtg
      781 tgagcactca atttcgtcga cagaagcatc cttaaactgg gtccaaggga aaactagcag
      841 gtcttgtacg aagtcggtgt cgttggtctt ggcgatatcg acgcccgtaa agccttctgg
      901 cttgttgtcg ccacaggcta gggagagctt gaggggcttc tttgatttag gggtggtttt
      961 ggtaggcatt ggttctcctt accacattag ttgattagcg gcatcatagt ggccgacttt
     1021 aacacgcgag tcacaggcaa atttgtatcc aaactcacgg gcgtggttaa agaaccacag
     1081 gtcctgagta aatacctggg ctccaccgcc gggcagtact tcctgcttgg ttttaaacca
     1141 gttgccatag tcaaagcgct tgtctcttag catttccatg cggaacaggg taaaacccat
     1201 gccgaggcca ttacagggag taatagtatc cggctgggga atacgaggga tgaaattgag
     1261 cggcatagcg gcagggtcgc cgtagcacat cggctggcca ccttcgcctt tggtccaata
     1321 taaaccgcca acagcatcgt actcatcgat tgattcgtat agttttagca gtccatcggc
     1381 tggtggggcg ttgtcttctt cgattgttag caaaaactta aacttggaaa gctcatcgtt
     1441 ttccagaatc atctttatga cctggttgta ggcctcgccc acttccatgc cttccataaa
     1501 caggggtcct agtgctttct ggttcatagg gcgaatcagg ccttgccagc tctgaacgat
     1561 gcgagctggg atcattccgc gagtagggca gacgatgatt gttgagaggt cacggtaggt
     1621 tttttcgcgc tctaagcgag tggcatagtc acttgcgttg tggatgccgg cattgtcagg
     1681 taccaggatt tggggttgtc ccatgatctt ttcgattata tcacgcccta atccaagcga
     1741 tagcagtagc cgcaactcgg ggcggcgaca gagtgatggt agcagcccaa tagtcagtcg
     1801 cagtgctacc gccaccggca gagcctacat taccaaaagc gcttgggttc tcgggaccac
     1861 tgctggtcag tagcttagtg gcaatggcac agtcgttacc cgtggcattg ttagtaacaa
     1921 tggtatagcc gctgggcgtg gtccagccgc cggtgtcgga accgttccag ttgtcgcaaa
     1981 tagccagtgc catggcactg ctggaaatag ccgtgagtag tggagggtcg gggtttatgg
     2041 aggtaccact ggcatcaggg gtttttgcgg aagcatcaat cgggttgctg gtatcgcagc
     2101 cgctaaatcc tacgatgtat agttcatagt agccgctggc accaaatgtc caggtgaggt
     2161 ttggggcgct ggcgccgcgg attatatagc ttaagcagta gttgaattgt gcagttgcgc
     2221 tggtgccgct aaaaatggtc gtccagcccg caggaggcgt gagggtgccg ttgttttcca
     2281 tctcgcagag tgcaatcagc gtatcacctt ggacggtccc cgtcggctcg gtgccagttg
     2341 gactgccgcc gttattgatg ttgtgagatt cgttacgaaa agcgattgcc atagacggtt
     2401 agtaggtagc gttaaagatt gcgttataga agttggcgga ctgcaaaaca gatgcagcag
     2461 tattgttaat ttgagcgcca gagatggtgg cgggaccggc cgaggttgcg gctgtgtaga
     2521 caccctcaaa cagcactacg tcacgcgcgg cagtagttcc actgccaata ggcgtcagta
     2581 ctgccgcacc actcgtagga gtaaacaaat tattgccgta gagcgtcata gtggcgcctg
     2641 gtgcagtcgt agcgctcgac ataacggcgg ctatccaata ttcaccgggg gtcatgttga
     2701 cattcatcgg tactgtcagc tgacgcatac cactaacact agtcagtgat gtgctgctaa
     2761 cactgctg
//
LOCUS       DATNGP010000157         2709 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_2077471, whole genome shotgun sequence.
ACCESSION   DATNGP010000157 DATNGP010000000
VERSION     DATNGP010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2709
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2077471"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..521
                     /locus_tag="VN778_04050"
     CDS             <1..521
                     /locus_tag="VN778_04050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50171.1"
                     /translation="VGSFVAFWPYFLSATDIKLLSLAFLAVAALSKYWHIFQAIHSVQ
                     RPTWGEFWFALVVGILAFDLGHPHIFTAALLEMSLADGLAAIIGTRYGNGQRYSVFGS
                     PKSVIGTLTFFVVSCFILGGYYLATPGVVAPVLGVASISLVAALLENVAVRGFDNIAV
                     PLFVAAALRFFS"
     gene            complement(514..2358)
                     /locus_tag="VN778_04055"
     CDS             complement(514..2358)
                     /locus_tag="VN778_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011667181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HXR50172.1"
                     /translation="MRRLLRFFTEYKLFSFAALGIIAGLVLELLKAHTAAHWVLGTIA
                     IIELFPLLWDMWNDIRSGVYGVDILAATAIITSVLLGQYWAGMVVVVMLTGGEALEDF
                     AHRRAQAELDELLEKAPSEAHVIRKGKTIDVKVEELHVGDKILIKPGEVVPVDAIIVE
                     GTASFDEASITGESLPQAKQVKDQLLSGAINIDGAVTAKATATAAESQYQQIIRLVRS
                     AASSQAPFVRLADRYSLPFTVTAFAIAGCVWAVSGHAIRFLEVIVVATPCPLILAAPI
                     ALISGMARASRYGIIVKTGSALERLAEAETIAFDKTGTLTRGELTIDHITSFGALSDD
                     EILALAAGLEQNSNHVVARAIVDGAQARNVKLKKAKHVQEIAGQGLKASLQGKEVLVG
                     RLSLIQEHEVKLPVTFKLESIRQTAVYVAVDNELVGAISFKDELRPEASATLKQLHKL
                     GLRHTLMITGDNKVTAETIAKQLGIDHVHAETLPADKLHVLEEIRDRPLAFVGDGVND
                     APALTAADVGIAIGARGSTAASESADMVIMLNDLSRVAVALSIAKYTFKIARQSILVG
                     IALSIILMLVFATGKFPPLTGAILQEVVDVFVIFNALRAHLFVPKTLS"
     gene            2442..>2709
                     /locus_tag="VN778_04060"
     CDS             2442..>2709
                     /locus_tag="VN778_04060"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HXR50173.1"
                     /translation="MNKITGRIFMKAAQIHSYGGKEVLQITSDAAKPQPAEGQILIEV
                     AAAGVNPFDVKVREGLARQMAELQFPATLGGDVAGTVAELGEGVS"
BASE COUNT          607 a          708 c          723 g          671 t
ORIGIN      
        1 ccgttggcag tttcgtggct ttttggccat attttttaag cgccactgac attaaattac
       61 taagtttggc ctttttagcc gtcgcggcgt tatcgaaata ttggcatatt tttcaggcta
      121 ttcatagtgt gcagcggccg acctggggcg aattttggtt cgccttagtg gtcggcattt
      181 tggcttttga ccttggtcat ccgcacattt ttacagcggc gcttctggaa atgagtttgg
      241 cggacgggct ggcggccata atcggcacgc gctatggcaa cgggcaacga tatagcgttt
      301 ttggctcgcc taaaagtgtg atcggtacgc ttacgttctt tgtcgtgtct tgtttcatat
      361 taggaggcta ttacctcgcc actcccggag tcgttgcgcc cgtgctaggt gtggcgtcga
      421 tcagcttggt agcggcgtta ctggagaacg ttgccgtgcg cgggttcgac aatattgcgg
      481 tgccactttt cgtggccgcc gcgttgcgct tttttagcta agagttttag ggacgaataa
      541 gtgcgcacgt agggcattaa aaattacaaa cacatcgact acttcctgca agatagcacc
      601 tgttagtggt ggaaacttac cggttgcaaa gactagcatc aagataatgc tgagcgcaat
      661 accgaccagg atgctttgac gggcaatttt gaatgtgtac ttggcaatag atagcgcgac
      721 agccacccgg ctcaggtcat tgagcataat gaccatgtca gccgattcgc tggccgccgt
      781 ggaaccacga gcgccaatag cgataccgac atccgcggcc gtcaaagccg gtgcatcgtt
      841 aacgccgtca cccacaaaag ccagcggccg atcacgtatt tcctctagga catgcagctt
      901 gtcggcaggc agtgtctcgg cgtgcacgtg atcgataccg agttgtttgg cgattgtctc
      961 ggcagtaact ttattgtcgc cggttatcat gagggtatgg cggaggccaa gcttgtgaag
     1021 ttgcttgagc gttgcgctag cttctggccg gagttcgtct ttgaagctga tggcaccgac
     1081 cagttcattg tcgacggcca catagacggc agtttgccgg atgctctcca gcttaaatgt
     1141 aaccggcagt ttcacctcgt gttcctgtat gagcgataag cggccgacaa gcacttcctt
     1201 gccttgcagg ctagctttca ggccctgccc ggctatttcc tggacatgtt tagccttttt
     1261 tagcttgacg tttcgcgcct gtgcgccgtc gacgatggcc cgggccacga cgtgattgga
     1321 gttttgctct aagccagctg ccagcgccaa aatttcgtca tcgctcagcg ctccgaagct
     1381 ggttatgtga tcaatcgtta gctcgccacg ggttagtgtg ccagttttat caaaggcaat
     1441 ggtttcggct tcggcaaggc gctccagcgc tgagccggtt ttgacaataa tgccgtaacg
     1501 gctggctcgg gccatgccgc tgatcaaagc aatcggtgca gccagaataa gcggacaggg
     1561 cgtggcgacg acaattactt ccaggaaacg aatagcgtga ccgcttactg cccagacgca
     1621 gcccgcaata gcgaatgccg tgaccgtaaa gggcaggcta taacgatcag ccagccgcac
     1681 aaagggcgct tggctgctgg ctgcacttcg cactagtcta atgatttgct ggtactggct
     1741 ctcagccgct gtcgctgtgg ccttagcggt tacggcacca tctatattaa ttgcgccact
     1801 aagcaactga tccttgacct gtttggcttg gggcaagctt tcgccggtga tggaggcttc
     1861 gtcaaaactc gctgtaccct caacgataat tgcatcaacc ggtaccactt cgcctggctt
     1921 gatgaggatt ttgtcgccga catgtagctc ctcgactttg acatcaatcg tcttgccttt
     1981 tcggataaca tgcgcctcag acggagcttt ttctagtagt tcgtcaagct cggcttgagc
     2041 ccgacgatgg gcaaaatcct cgagcgcttc gccgccggta agcattacaa ccaccaccat
     2101 acctgcccag tactggccta agagcaccga ggtaataatt gccgtggccg ccaaaatatc
     2161 gacgccataa actccggaac gaatgtcatt ccacatgtcc caaaggagcg gaaagagttc
     2221 gataatagct atcgtgccaa gcacccagtg cgcggcagtg tgtgccttga gcagctcaag
     2281 gaccaagccg gcgatgatac ccaacgcagc aaaactaaac aatttgtact cagtgaaaaa
     2341 tctcagcagc cgtctcataa aaacctcatt tgtttcttaa acctgattac aaccagtata
     2401 ccagttgcgc cgcgccgaca cttactatat tatgtatagt attgaacaaa ataactggga
     2461 ggattttcat gaaagcagcc caaatccaca gctacggcgg taaagaagta ttacaaatca
     2521 ctagtgatgc agccaagccg cagcccgctg aaggccaaat actgatagaa gtcgcagctg
     2581 ctggcgtgaa cccttttgat gtgaaggttc gtgaaggcct cgcgcggcaa atggctgaac
     2641 tccagtttcc cgccacgctt gggggagatg tcgccggcac cgtcgccgaa ctgggtgagg
     2701 gtgtcagcg
//
LOCUS       DATNGP010000158         3112 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_2259803, whole genome shotgun sequence.
ACCESSION   DATNGP010000158 DATNGP010000000
VERSION     DATNGP010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3112
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2259803"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..407
                     /locus_tag="VN778_04065"
     CDS             <1..407
                     /locus_tag="VN778_04065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50174.1"
                     /translation="SSLVTWVESDDTELAIVTSDQFKDFQRARSKTRVPGRSFNWALR
                     ALNPSSAPTQRLQQRQDLGQYDLAQTSDYSYADNNIARGLIVDTLPMLVSGLRTGELR
                     IGRAGDEVVRDLGTYCLALFADRQPVSLVSAV"
     gene            501..980
                     /gene="rpsP"
                     /locus_tag="VN778_04070"
     CDS             501..980
                     /gene="rpsP"
                     /locus_tag="VN778_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020819070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HXR50175.1"
                     /translation="MLVIRMQRTGRKGHAMFRVVVQDSRRTPSSGKIVTQLGSYDPHT
                     KAVTLNKERAQHYLDHGAQPSDRVARLLKAEGVKLPAWVKLQPDKERTVRNPDKRRST
                     RPEEPAAEEAPTAEAEETATEPEAAPEEASEPEAAEAAEEPAEAEATEAPADESTEA"
     gene            1131..1586
                     /locus_tag="VN778_04075"
     CDS             1131..1586
                     /locus_tag="VN778_04075"
                     /inference="COORDINATES: protein motif:HMM:NF024483.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HXR50176.1"
                     /translation="MNNIDQQFIEYVVKSLVGKPEAVKVDRRIDEKGVLLELTVDPED
                     LGRVIGKRGATAQSLRTLLRALGTKNDARYNLKIVDNGVGSHSSSYAGTSDDSSGDDT
                     TADEPDTPAESVEEEVKSAQEESQKKEAPASDRLAQTRQELADLDDLDV"
     gene            1609..2265
                     /gene="trmD"
                     /locus_tag="VN778_04080"
     CDS             1609..2265
                     /gene="trmD"
                     /locus_tag="VN778_04080"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HXR50177.1"
                     /translation="MKIQIITLFPEMFTGVLHSSMLWKAAAHDIVSYEFINLRDFGLG
                     PRKQVDDTPYGGGDGMLLKPEPVVAAIEEAKSKSPEASVLLPTPRGAMYKQSDARRLA
                     GKAQDLIIVCPRYEGYDERITDWIDERFCIGNYVLTGGELPAMIIIDSVVRLLPGVLG
                     GESSAEIESFQADDQTIEFPQYTRPEEFRGLKVPEVLLSGHHAEIERWRKSHSRRSEQ
                     "
     gene            2361..2624
                     /locus_tag="VN778_04085"
     CDS             2361..2624
                     /locus_tag="VN778_04085"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HXR50178.1"
                     /translation="MAPKLRPGQIVWATTLFRKLHPGQVVIIAQDNKEFIKRIERIEK
                     TDNEEERLFVVGDNLDVSTDSRQFGWLDRRSVVARVFKPNLAK"
     gene            complement(2632..>3112)
                     /locus_tag="VN778_04090"
     CDS             complement(2632..>3112)
                     /locus_tag="VN778_04090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="EmrB/QacA family drug resistance transporter"
                     /protein_id="HXR50179.1"
                     /translation="TNRAILGVWMAILGIGIGMVMPVLTLAVQNAVERKDLGTATSTV
                     TFFRSIGSSLGAAIFGAILINRLTTNLQSSLPGASGAHLAKSLQSSSAQLKSAPPAIV
                     HTVLTAFASAFSDVFLIAIPFAIVAFLVSLFLKESPLRESSRAFAEGEGFEPARSLE"
BASE COUNT          798 a          774 c          870 g          670 t
ORIGIN      
        1 actcttcttt ggtgacctgg gttgagtctg acgacacaga gctggccatt gttacgtcgg
       61 atcagtttaa agatttccag agagcccgaa gcaaaacgcg ggtgccagga cgtagcttta
      121 actgggctct tagggcatta aacccatcga gtgcgccgac gcaaaggtta caacagcggc
      181 aagatttggg tcagtatgat ttagctcaaa cgagcgatta tagttacgca gataacaata
      241 tagctcgggg tttaattgtt gacaccttac cgatgcttgt cagcggttta cgcactggcg
      301 agctgaggat tggcagagct ggcgatgaag ttgttcggga tttagggact tactgcttgg
      361 cgctgtttgc tgatcgccag ccagtttcac tagtttcagc tgtctaatga tgtatttgac
      421 aacagaggcg gttatctgta aaatacatta gattgtcttt ataaactttt aatcgtagag
      481 acgaagaaag tagagtttac atgttagtta ttcgcatgca gcgtactggc cgcaaaggcc
      541 acgccatgtt ccgcgttgtc gttcaagact cccgccggac cccaagcagt ggcaaaattg
      601 tcactcaact gggaagttac gatccacata ctaaagctgt cacgctcaac aaagagcggg
      661 cgcagcacta cctcgatcac ggcgcgcagc cgtcagatcg cgttgcccgg ctgctcaagg
      721 cggaaggcgt gaagctaccg gcttgggtca agctgcaacc tgataaagaa cgcacagtcc
      781 gtaaccctga caagcggcgc agcactcgtc ctgaagagcc ggccgccgaa gaagcgccta
      841 ctgctgaagc tgaagaaact gctaccgaac ccgaagctgc tcccgaagaa gcgagcgagc
      901 ctgaagctgc tgaagctgcc gaagaacccg ctgaagccga ggccacagaa gctccagccg
      961 acgaatccac tgaggcttaa gtagcccgct gggcaaaaaa ttttacaatc cgactttgaa
     1021 cctttgctat aattaaagaa cgctaggacg ttcacacttt ggtcagttta ctgctaatga
     1081 ccgctagtac cggaggtggg aatactaaca taacttgagg agcctgttac gtgaacaata
     1141 tcgatcagca attcatagaa tatgtcgtca aatccttagt cgggaagcct gaagccgtta
     1201 aagttgatcg ccggatcgat gaaaaaggcg tgctgctgga gttaactgtc gacccggaag
     1261 atctgggtcg cgtcattggt aagcgtggcg ctacagccca gtctttgcgc actcttttac
     1321 gtgcactggg gacgaaaaac gatgcccgct ataacctgaa gattgtcgac aatggggttg
     1381 gtagccactc ttcctcgtac gccggcacca gtgatgatag cagcggcgac gacacgactg
     1441 ccgatgagcc ggacacaccg gccgagagtg tcgaggaaga agtcaagagc gcccaggaag
     1501 aatcgcagaa gaaagaagcg ccggcaagtg atcgcctagc ccaaactcgt caggagttgg
     1561 ctgatctcga cgacctcgat gtataagatt gtgtcctggt agcaacggat gaaaattcag
     1621 attattacgc tttttcctga gatgttcact ggcgtgctac atagcagcat gctgtggaag
     1681 gcagccgcgc acgatatcgt gtcgtacgaa tttatcaact tacgggactt tggcttaggc
     1741 ccccggaaac aagtggatga tacgccatac ggtggcggtg acgggatgct cttgaaaccg
     1801 gaaccggtag tagccgctat cgaagaggca aaaagcaaaa gtccggaggc aagcgtatta
     1861 ctgccgacgc cgcggggggc aatgtacaag caatcagatg cgcggcgctt ggccggcaaa
     1921 gcccaagacc tcatcattgt ttgtccgcgg tacgaaggct acgacgagcg tatcaccgac
     1981 tggattgatg agcgtttttg tatcggtaac tacgtactga ccggtggtga attgccggcc
     2041 atgattatta ttgactccgt cgttcgctta ctgccagggg tgctgggggg cgaatcgtcc
     2101 gctgaaatcg aatcatttca agctgacgac cagacgatcg aatttccgca gtatactcgg
     2161 ccagaggagt tccgaggctt gaaggtgccg gaggtgctgc tgagcggcca tcacgcggaa
     2221 attgagcggt ggcggaaatc tcacagccgg cgttcagagc aataagttac atttaactca
     2281 cgagagggag attgcaaatg caggtgcatg taccgacact gactaacccc attctaatta
     2341 gaagagttgt cagtcccagt atggccccca agctgcggcc gggccaaatc gtctgggcca
     2401 ctaccttatt ccgcaagcta caccccggtc aagtcgtgat tatcgcgcaa gacaacaaag
     2461 aatttatcaa gcgtattgaa cgcattgaga aaaccgacaa tgaggaagag cgtctgtttg
     2521 ttgtcggcga caaccttgat gtcagcaccg acagccgcca gttcggctgg ctcgaccggc
     2581 ggtcggttgt cgcacgcgtt ttcaaaccga atttagctaa gtagttcgga tttattcaag
     2641 gctgcgggcc ggttcgaagc cttcgccttc ggcgaacgca cgactcgact cgcgtaatgg
     2701 tgattctttt aggaatagcg acaccaggaa ggccacgata gcgaacggga tagcaatcaa
     2761 aaagacgtcg ctaaaggctg aggcgaaagc ggttagcaca gtatgcacga ttgccggcgg
     2821 cgcacttttg agctgcgccg acgaagactg caagctcttg gccagatggg cgccgctggc
     2881 tcccggcaag ctggattgca agttcgtcgt taagcgattg atgaggatgg cgccaaaaat
     2941 ggcagcccct aagctgctac cgatactgcg gaagaatgtg accgtcgaag tggcggtgcc
     3001 aagatctttg cgctcgacgg cattttggac ggccagcgtt aagactggca taaccatacc
     3061 aataccgatg cccaagatgg ccatccacac acccagaatt gcccggttgg tg
//
LOCUS       DATNGP010000159         2174 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_4579883, whole genome shotgun sequence.
ACCESSION   DATNGP010000159 DATNGP010000000
VERSION     DATNGP010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2174
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4579883"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..367
                     /locus_tag="VN778_04095"
     CDS             <1..367
                     /locus_tag="VN778_04095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50180.1"
                     /translation="TDTIYVLPIIGAVYVVETGSVIVNRLSRKLRHGKKVFLSSPIHH
                     HFEAIGWPETKVTMRFWILGQVAGVLGLIVFLLGRRSCGHRFLVVAHNSGSSSWTWGA
                     STSQTIGCSSCAPCYWLLA"
     gene            370..1482
                     /locus_tag="VN778_04100"
     CDS             370..1482
                     /locus_tag="VN778_04100"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative peptidoglycan glycosyltransferase FtsW"
                     /protein_id="HXR50181.1"
                     /translation="MVYAISPALAQANHVSNTYYVSKQLIAIGLSLGAFAVTSQVPLQ
                     WWRQAYKPLLIIAGLATLLALALPVNPAYPAHRWLRLGSFSFQSVELLKFAIMIWLAG
                     FLAERLKQGNITVTQTTLKPILIALGGIGVIVAGVQSDLGSTGVIIVMMATMAYVAGL
                     PMKRIMMIGGVVLIGLILAVSAIPYRRARLEAYLHPSSNCQTTGYQACQALIAVGSGG
                     VIGLGLGSSVQAYGYLPEAANDSIFAIYAEKFGFIGSVVLLAVFVAFFARLKSIAERL
                     TDDFSRLLVMGILAWLSVQALVNVGAMIGLLPLKGITLPFISYGGTSVVFAAAAVGLA
                     FQASQYAAFVAPRIGASETGRPSRLQNSSLRKANYS"
     gene            1479..>2174
                     /locus_tag="VN778_04105"
     CDS             1479..>2174
                     /locus_tag="VN778_04105"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXR50182.1"
                     /translation="MKQTVVLTGGGSGGHITPLLAVAAELKRQKPELQLIYIGQRGDK
                     LGDIPAQSPHIDEVFSIRAGKFRRYHNEGLKQLLDIPTLMLNIRDALYALIGIWQSRQ
                     LLKRLDPQIIFIKGGFVGVPVGLAAASLKIPYVTHDSDAIPGLANRLIARWATRHAVA
                     LPAEVYTYPPSKTVTTGIPLQADFVPLTASLFKKYRAEIDVPLKAKLLFIIGGGLGAQ
                     RVNRAVAEAVPHLL"
BASE COUNT          426 a          584 c          613 g          551 t
ORIGIN      
        1 gacggataca atttacgtgc tgcctattat tggggcagtc tatgtggtcg aaaccggttc
       61 ggttattgtc aatcgcttgt cgcgcaagct tcgacacggc aaaaaagtct ttttgtcatc
      121 gccgattcat catcacttcg aagccattgg ttggcccgaa actaaggtga cgatgcgatt
      181 ttggatcctc ggccaagttg ccggcgtgtt aggtctcatt gtgttcttgc tagggaggcg
      241 ctcatgcggc cacaggttcc tcgtcgtcgc ccacaactcc ggaagcagca gctggacctg
      301 gggcgcaagc acaagccaga ctattggctg ctcatcttgt gcgccttgct actggctgtt
      361 ggcctgattg tggtctatgc cattagtccg gccctggctc aggccaatca cgtcagcaat
      421 acatactacg ttagcaagca gctcattgcc atcggtttga gtttaggggc gtttgcagtg
      481 acctcacaag tgccgctgca atggtggcgg caagcgtaca aacctctcct gatcatcgct
      541 ggcctagcga cgctcttagc cctggcctta ccggtcaatc cagcttaccc ggcgcaccgc
      601 tggctgcgtt tgggttcatt ttcgtttcaa tcagtagagc ttttgaagtt tgccatcatg
      661 atctggctgg ccggcttttt ggcagaacgc ttgaaacagg gcaatattac ggtgacgcaa
      721 acgacgctca agcctatctt gatagcgctc ggcggcatcg gtgtgatcgt ggccggcgtg
      781 cagagcgatc tcggctcaac tggcgtgatt atcgtcatga tggccaccat ggcctatgtc
      841 gccggcttgc caatgaaacg tattatgatg attggcggcg tggtgttgat cgggctaatt
      901 ttggcagttt cggccattcc gtaccgccgg gctcgactgg aagcttattt gcacccttca
      961 tctaactgcc agaccacggg gtatcaggcg tgccaggcgc tgattgccgt cggttcaggc
     1021 ggggtcattg ggctggggct gggcagcagc gtacaagcct atggctattt accggaagcg
     1081 gccaacgact caattttcgc catttacgcc gagaagttcg gcttcatcgg cagcgtcgtc
     1141 ttgctggcag tgtttgtggc gttttttgcg cgcctcaaat cgattgctga gcgtttaacg
     1201 gatgacttca gtcggttgct ggtaatgggc attctggcct ggctcagcgt ccaggcgtta
     1261 gtaaatgtcg gggctatgat cggtttgcta ccactcaaag gcatcacgct gccatttatt
     1321 agctacggtg gtaccagcgt ggtctttgcg gccgcggccg tcggtttagc cttccaagcc
     1381 tcgcaatacg cagccttcgt ggcacctcgt atcggcgcga gtgaaaccgg acggccctca
     1441 cgcttgcaaa acagcagcct tcggaaggct aattattcgt gaagcagacg gttgttctga
     1501 cgggcggtgg ctcgggtggc cacattacgc cgctgttggc ggtagccgct gagctaaagc
     1561 gccaaaaacc cgaattacag ctaatctaca tcggtcagcg cggggacaag ctgggcgata
     1621 ttccagctca gagccctcat atcgacgaag ttttctcgat cagggcgggg aagttccgcc
     1681 gctaccacaa cgaaggctta aagcagctct tagacattcc gacactcatg ctcaacatcc
     1741 gcgatgcgct ttatgctctg attggtatct ggcaaagccg tcagcttctc aagcgactcg
     1801 acccacagat tattttcatc aaaggcggtt ttgtaggcgt gccagttggt ctggcggctg
     1861 ctagcctgaa aataccgtac gtgacgcacg attctgatgc tattccaggc ctggcgaacc
     1921 gccttattgc tcgctgggct accaggcatg ctgtggcttt gcccgctgaa gtctatacgt
     1981 atccgcccag taagaccgtg actaccggta taccgttgca ggctgatttt gtgccgctga
     2041 cggcttcttt atttaaaaag taccgggctg aaattgacgt tccacttaag gccaaattgc
     2101 tattcatcat cggtggcggc cttggagcgc agcgcgtcaa tcgagcggta gcggaggccg
     2161 tcccgcatct gttg
//
LOCUS       DATNGP010000160         2629 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_3883844, whole genome shotgun sequence.
ACCESSION   DATNGP010000160 DATNGP010000000
VERSION     DATNGP010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2629
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3883844"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="VN778_04110"
     CDS             complement(<1..221)
                     /locus_tag="VN778_04110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50183.1"
                     /translation="MPIVKGGAMANTSSYYYGYYPDDYGYYWWGAPYGVSDYDLCDYN
                     NNWVLDDNEIADEVRDSIYADQGITASDT"
     gene            complement(379..1443)
                     /locus_tag="VN778_04115"
     CDS             complement(379..1443)
                     /locus_tag="VN778_04115"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HXR50184.1"
                     /translation="MKPNIKKIVGQGKRLASERLRKPEPTAEELRVTNETVAERREEV
                     LRDARKFIYPLGAPKHRIVKWSVGILIAAIVVFFSFCLLELYKFQDTSTFIYGVTQVV
                     PFPVAFVDHHFVSYNDYLFELRRYMHYYATQQNIDFSTKAGKQQLVVFKQRAFDQVIQ
                     QAYVAELADKNNITVSDKDVDAAVNLVRDQNRLGASDEVFQSVLSEFWGWSVADFQRE
                     LRQEIMAQKVVSQLDTATHTRANAAFDQLQKGVDFGQLAKQVSDDQSTRAQGGDFGAL
                     IDRANTQLAPQVTEALFQLSPGKYSWIINTGYSLEIVKVLEVKGTQLRAAHISFNFQP
                     ITAYTDPLKKSEPVHPLVRF"
     gene            complement(1451..2032)
                     /locus_tag="VN778_04120"
     CDS             complement(1451..2032)
                     /locus_tag="VN778_04120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HXR50185.1"
                     /translation="MAEDQKKPKPVKYFDSQFDDEEVLYVFRRHPVIMRTGLVVSMSL
                     WLVGPLIILALTYIMPNNPPSMVAFFLAVIGSIVFGSLVILPWWIGWYFSLFIMTSQR
                     FVQITQKGLFHTSFADISLPQIQQVNYEVSGLQETLLGFGTITMQTYIGELVIHDVPH
                     PAKIQRKIVQILRDQGVTPVALGFQRGIPERVK"
     gene            2098..2343
                     /locus_tag="VN778_04125"
     CDS             2098..2343
                     /locus_tag="VN778_04125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50186.1"
                     /translation="MNTIQYTIRLIPPKIDAALRQQARRSGKSLNEVVVDTLAKGTGM
                     KPDESFTDLDWFIGKGSLGDAFVDSLAWLDKAPKEIK"
     gene            2310..>2629
                     /locus_tag="VN778_04130"
     CDS             2310..>2629
                     /locus_tag="VN778_04130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50187.1"
                     /translation="MARQSAEGNQVTPKLALDTNAYRALDDGNTKLAGNVKVAAQIGI
                     PMTVLGELYFGIYAGSKQDSNLSNLSRFLASPRVELLQIDEQTAKIFGEVATELKRLG
                     KPIQ"
BASE COUNT          662 a          678 c          664 g          625 t
ORIGIN      
        1 ttagtgtctg acgcagtaat cccttggtcg gcgtagatgc tatcgcgtac ttcgtcggca
       61 atctcgttgt cgtcaagcac ccaattgttg ttatagtcgc ataagtcata gtcactcacg
      121 ccgtacggag cgccccacca gtagtagccg tagtcgtctg gatagtaacc gtaataatat
      181 gagctcgtgt ttgccatagc gcctcctttc acgataggca ttactgatac atttttcggg
      241 ctagttttct gtttctacaa ggcgtagtct aaccctgtct acggctgcct gtctgtaaaa
      301 ttggcagcag gaataaggag atcgaagccc gagctgccga tccgactgtg gaccgcacac
      361 ccccctcgtg cgcggtgatt aaaacctgac tagcgggtga accggctcgg attttttgag
      421 tgggtcggtg taggccgtaa ttggctggaa gttgaacgag atgtgggcag cgcgcagctg
      481 ggtgcctttg acttccagta ccttgacgat ctccaggcta taaccagtgt tgatgatcca
      541 tgaatactta cccgggctca actggaaaag cgcctcggtc acctgtggcg cgagctgggt
      601 attggcacgg tcgatcaagg cgccgaaatc tccgccttgc gcgcgtgttg actgatcatc
      661 cgacacctgc ttggcaagct ggccaaaatc tactcccttt tgcagctggt caaacgccgc
      721 attggctcgt gtatgggtgg cagtatcgag ctggctgaca accttctggg ccatgatttc
      781 ctggcgtaat tcacgctgga aatcagctac cgaccagccc cagaactcac tcaaaacact
      841 ctggaaaact tcgtcgctgg cacccaaacg gttctggtcg cgtaccaaat taactgccgc
      901 gtcgacatcc ttatcactga cagtgatgtt attcttgtca gccaattcag caacgtaggc
      961 ttgctggatg acttggtcaa aagcccgctg cttgaacacc accaactgct gcttacccgc
     1021 cttggtactg aagtcaatat tttgctgtgt cgcatagtaa tgcatgtagc gtcggagctc
     1081 gaataagtag tcgttatatg agacaaagtg gtgatcgaca aaggccacgg ggaacggcac
     1141 gacttgtgtt acgccataaa taaaggtcga cgtgtcctgg aatttgtaca actcgagtag
     1201 acaaaagctg aagaacacga caatagcggc gatcaggatc ccaaccgacc actttacgat
     1261 ccggtgcttc ggcgcaccga gtggatagat aaacttccga gcgtcgcgta agacttcttc
     1321 gcggcgttca gccaccgtct cgttggtaac ccgcagctct tcggctgttg gttcaggctt
     1381 ccgtagccgc tcgctagcca agcgcttgcc ctggcccacg atcttcttga tattaggttt
     1441 cattgaatac ttatttcacc cgttctggta tgccccgttg gaatcctagc gcaaccggcg
     1501 ttacgccctg gtcgcgcaag atctggacaa ttttgcgctg gattttggct ggatgcggca
     1561 catcgtgaat caccagctca ccaatgtagg tttgcatagt aattgtacca aacccgagca
     1621 gtgtctcctg cagaccggaa acttcatagt tcacctgttg gatctgaggc aaggaaatat
     1681 cagcgaagct ggtgtgaaac aaaccttttt gggtgatctg cacaaaccgc tggctagtca
     1741 tgataaacag gctgaaatac cagccgatcc accacgggag aataacaagc gaaccaaaaa
     1801 caatgctacc aatgaccgct agaaaaaacg ccaccatcga tggtggatta ttaggcatga
     1861 tataagtaag tgccaggatg atcagcggtc cgaccagcca cagcgacatg ctgactacca
     1921 gcccagtacg cataatgacc ggatggcggc ggaagacgta cagcacttcc tcgtcgtcaa
     1981 actggctgtc aaaatacttg accggctttg gctttttctg gtcttctgcc ataagagtat
     2041 tgtaacagca acagaagtgc attgactagt gaatgcgttg aatgctatat tgaatgcatg
     2101 aatacaattc aatacactat cagactgatt ccgcccaaga tcgacgcagc attacgccag
     2161 caagcgcgac gatccggcaa aagcctcaat gaagttgtcg ttgacacctt ggcgaagggc
     2221 acaggtatga agcccgatga aagcttcact gacctagatt ggtttatcgg caaaggatcg
     2281 cttggtgacg cctttgtaga ttcgctagcg tggctcgaca aagcgccgaa ggaaatcaag
     2341 tgacgcctaa gctagcactc gacacgaatg cctaccgggc actcgatgac ggcaacacaa
     2401 aacttgccgg aaacgtgaaa gttgctgcgc aaattggcat accgatgacc gttttaggtg
     2461 agttgtactt tgggatatac gcaggtagca aacaagatag caacctctca aatctaagca
     2521 ggttcttagc ttcgccccgt gtcgagctgc tgcaaatcga cgagcagact gctaaaattt
     2581 ttggcgaagt tgcaaccgaa ctcaagcggc ttggcaagcc aatccagca
//
LOCUS       DATNGP010000161         2517 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_2953725, whole genome shotgun sequence.
ACCESSION   DATNGP010000161 DATNGP010000000
VERSION     DATNGP010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2517
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2953725"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..694)
                     /locus_tag="VN778_04135"
     CDS             complement(<1..694)
                     /locus_tag="VN778_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007955921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXR50188.1"
                     /translation="MALIELREVSKLYGFGDATTLALDEVSLSVEQGEFMAIMGPSGC
                     GKSTLMHIIGLLDRPTHGDYLLNGRNVARLRPSQAARIRRDTIGFIFQAFNLIPRMTV
                     LENVALPLVYKGYPLTKRLQIASSILERVGMKEREYFYPSQLSGGQIQSAAIARALVT
                     DPKLIIADEPTGNLDSANTRLVMELLSEVHRMGNTVLFVTHNPELTRYASRVVYMRDG
                     VIVHDEVTTIGDI"
     gene            840..1037
                     /locus_tag="VN778_04140"
     CDS             840..1037
                     /locus_tag="VN778_04140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50189.1"
                     /translation="MKGNYTAILLEEIRDQYRTVLEAVGDMQTKVARIPVIEQDIAEL
                     KTDMKAIKFVLTEQNRTIDDH"
     gene            1260..1349
                     /locus_tag="VN778_04145"
     tRNA            1260..1349
                     /locus_tag="VN778_04145"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1294..1296,aa:Ser,seq:gga)
     gene            1933..2091
                     /locus_tag="VN778_04150"
     CDS             1933..2091
                     /locus_tag="VN778_04150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50190.1"
                     /translation="MKRLHDAHDEEAVLAEEVEHLEWLQSGIADGTGDGDNALYHAQD
                     SSDSADNT"
     gene            2313..>2517
                     /locus_tag="VN778_04155"
     CDS             2313..>2517
                     /locus_tag="VN778_04155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50191.1"
                     /translation="MSATGQIVISNTQVQDITENSAMISWTTSIPTVSKVRFGLTTQY
                     GAVASSPLLTTQHRITASSALLMS"
BASE COUNT          624 a          648 c          634 g          611 t
ORIGIN      
        1 ctatgtcacc gatagtcgta acttcatcat gtactatgac gccgtcgcgc atgtagacca
       61 cacggctggc atagcgcgtt agttcaggat tatgggtcac aaacagcacg gtgttaccca
      121 tgcggtgcac ctcactgagt aactccataa ccagacgggt gttcgcgctg tctaaattac
      181 cggtcggttc atcagcgatg attagcttcg ggtcggtaac gagcgctcgg gcgattgcgg
      241 ctgattggat ctggccgccg ctcagttgcg aaggataaaa gtattcacgc tctttcatgc
      301 cgacgcgctc caaaatactg ctggcgatct gcaggcgttt cgtcaacgga tagcctttat
      361 acactagggg cagggcaaca ttttcgagta ccgtcatacg cgggatcaaa ttaaacgctt
      421 ggaatataaa gccaatcgta tcgcggcgga tccgggccgc ctggctgggc cgcaaccgtg
      481 ctacattccg cccgttcaaa agataatcac cgtgcgtcgg gcggtcgagc agaccaatga
      541 tgtgcatgag cgtgcttttc ccgcagccgc tcggccccat gatggccata aattcaccct
      601 gctcaacgct caaactaact tcgtcgagcg ccaatgtcgt ggcgtcgccg aagccataga
      661 gcttactaac ttctcgcagt tctattagag ccattttata tagtgtatgc cgccgatcca
      721 gactcagaca agaggttttt cagtaaatca tccaaacttt tcacggggtt ttcacgacca
      781 gagtaaaatt caggaatttg tcattttgtg gccagcactt cgggtgctag gctagcatta
      841 tgaagggcaa ttacactgca atcttactcg aagagattcg ggaccaatat agaaccgtgc
      901 ttgaagcagt tggcgacatg caaaccaaag ttgctcgtat ccccgtaatc gagcaagata
      961 tcgccgagct caaaactgat atgaaggcga tcaagttcgt cctcaccgaa caaaaccgca
     1021 ccatcgacga ccactgacac cgcatcaccc gcatagaaca tctcaaactc gcctaaatct
     1081 agtataattc ccctgagatc ctctacaggg atttgttgtt tgcagttcat gcaaattcac
     1141 aaaccaagga gcaaggtcaa ctgtttgtga atttgggacc aggagcagca gagagcttgc
     1201 ttggtaaagt ggcaaacttg ttttaccact ttgccgagca agctcgtcgc ttgctcggag
     1261 gagaggtcgc atagtggcct agtgcgccgc cttggaaagg cggtatgggt gcaaatccat
     1321 cgagggttca aatccctccc tctccgccaa agaagagtga cctgccgcaa ggtgggtcac
     1381 tcttctttgc tcgtgaaagg ggatttgaac ggacagtcca gtggactgtc ccggcacttt
     1441 tgccaactgg cttggcaaaa gcaaatccct ccctctccgc caaaataaaa cacctgccgt
     1501 atggtgggtg ttttattttg gggcttgtgt cgggggattt gaacgcgagc gcctgtggcg
     1561 ctcagccgcc cgtagcccgg tgaccatgga gccgggtgac tgtagcaaaa gttaattttc
     1621 ttttgctcag tcaggtcaaa tccctccctc tccgccaagt cacaatcaat tgtgacttgg
     1681 cgtaaacata catttttaca ctagacacgg ttatggtttg gtggtaattt gtatgcaaat
     1741 taaggagggg tcatgggagc taacgaaaaa gcaaggcgta gtccgggaga agaagcagat
     1801 gctttaatta cggtagccgc aactacaagc atcgatgctc agcttacgct tctttatgca
     1861 cgtctggcag tgctaaattc cttgatagag tcccttagtc cgtctgggcc aggtagtctg
     1921 tcgcctgatt cattgaaacg tctccacgat gcccacgatg aagaagcagt tctagcagaa
     1981 gaagttgaac acctggagtg gttgcaatcg ggtattgcag acggaaccgg tgacggcgat
     2041 aatgccctat atcatgccca ggacagctca gattcagcag acaacacata gcagagcgta
     2101 aaagactttt agcccttact ttttggctcg ttcaatgctt gtgtaatttt tcccgcgcct
     2161 gatcaagcac agagtataga cacgtattct acagtcccga ggaggaataa tagcaagtgc
     2221 gctacactaa gcatatgcgg gttcgtacgc tctgtagtgg ttttgtgttg ttggtaggca
     2281 gttttgccgt gtccgtgtta tgcgttcttc cggtgagcgc aaccgggcag atcgttatct
     2341 ccaataccca ggttcaagac atcaccgaaa atagcgcgat gatcagctgg acgacatcca
     2401 tcccgaccgt atcgaaggtg cgattcggcc tcacaaccca gtacggcgca gttgctagca
     2461 gcccattgct caccacgcaa catcgcataa cggccagctc ggcactcctc atgtcag
//
LOCUS       DATNGP010000162         4955 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_1427289, whole genome shotgun sequence.
ACCESSION   DATNGP010000162 DATNGP010000000
VERSION     DATNGP010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4955)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4955
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1427289"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..509)
                     /locus_tag="VN778_04160"
     CDS             complement(<1..509)
                     /locus_tag="VN778_04160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50192.1"
                     /translation="MMKIKNKQDGFTHIILLAVIGVLVVGILSAAYVKVNNASDKKSV
                     HSDATKNTNTSKKTTVTPVALAPATKATVATPQTTATAPTASPTPTVTPKKTAPIKTS
                     PVSNIVHPTIANCNRQNFTVYASVPSGTPAYSEGNLSQVLYTWPYGSAITDVYCDSGD
                     SKQMVFRID"
     gene            704..979
                     /locus_tag="VN778_04165"
     CDS             704..979
                     /locus_tag="VN778_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012506989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HXR50193.1"
                     /translation="MSTTQAFKLDDETNERLKALAQQRDRSAHWLLREALQRYLTEEE
                     RYEREKAEDLAEYKDYLLTGKAVDSETVTSWLNELASGKKTTWPKQK"
     gene            961..1260
                     /locus_tag="VN778_04170"
     CDS             961..1260
                     /locus_tag="VN778_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012465098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HXR50194.1"
                     /translation="MAKAKVVWRERALEDIDRLYDFLFKKNEEAATKVAQVILRGSLL
                     LEESPRLGRPMADGSERRELFIPFGAGFYVIRYFLADDIVVIVRVWHGREDRKAD"
     gene            complement(1311..1385)
                     /locus_tag="VN778_04175"
     tRNA            complement(1311..1385)
                     /locus_tag="VN778_04175"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1350..1352),aa:Glu,seq:ttc)
     gene            complement(1836..2978)
                     /locus_tag="VN778_04180"
     CDS             complement(1836..2978)
                     /locus_tag="VN778_04180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50195.1"
                     /translation="MKITRILASAVVTVMAFGVSGVAHANYYPLDRPTYTCTTPTNCT
                     GADHVTFDSFTNNPVVGDERPFLAGSLNGANVQDRLQVKDGDVVTVRAYIHNNADASL
                     MGGEDKTVAKNVNFRLLVPTASQSDTNIFGFITASNATPAMVNDTMSVYADSAFTLEY
                     VKGSAQWSHAADGVHQTTVTLSDDIAGANGTSLGDLSGCFAYSGYVTVKVKVHMPTTP
                     PPVTPAYSCDLLHVVADNATRKVVVDQFKTTQTGGATFQNAVIDWGDKTKTSPLTTLI
                     GQPHTYTADGSYTIVATANFGVNGATKSASSQGCTQTVTFKTTTPPVTPPVTPPVTPP
                     TTPPTTPQPQQLPNTGAGDTIAIFASVVVAGTIGYRLLLNRKLARR"
     gene            complement(3475..4491)
                     /locus_tag="VN778_04185"
     CDS             complement(3475..4491)
                     /locus_tag="VN778_04185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50196.1"
                     /translation="MSRNKSKIAGPVIAIIGVIVVLVALCVTAATGALSAPASVTATS
                     CATKPVPASADIAPNGVKYDAYDQTVEGEFGDGTTSTKQSEVITWAGDKLIHDPQFAA
                     IVVTASQLPKDTANVPKALESKFNDIDATASKYAADDTAWAGALETLYGDAVSCKLVT
                     YDVRGFYTLEMHVNKANPAKPAPKLTFNSVSDESLGESLDITMKSGLVVHLRLVCGGQ
                     PSQPYQPATPIRGPTTAPTHPHPTTPVPTPTSSTPTTVPTTAPTTHPTTVPSTTHTTT
                     SLTCDQFEHGVGYIGTPPNDCHKNGNSQPTSQPPSGHGTNPPPADPSSSGCAAPDPTT
                     GRCP"
     gene            complement(4568..>4955)
                     /locus_tag="VN778_04190"
     CDS             complement(4568..>4955)
                     /locus_tag="VN778_04190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50197.1"
                     /translation="TETDRRFADHDERITRAHALGVVAVDALKAQDKKIDAIGRVRKG
                     LLWIPIVVGILTWFVCQWIGHRASKVYGMGNQAHTQTGMSAAPWGWIGLGIALVMSAL
                     VLLLVQAIPAKPEKPVQQATTDGPRH"
BASE COUNT         1136 a         1213 c         1418 g         1188 t
ORIGIN      
        1 ttatctatgc gaaagaccat ctgcttacta tcgccggagt cacaatacac atcagtaatt
       61 gccgagccat atggccacgt gtacagcact tgagaaagat taccttcgct atatgccggt
      121 gtcccggaag gtacactagc atacacagtg aagttctgtc gattacagtt ggcaattgtg
      181 gggtgaacga tatttgatac cgggcttgtc ttaatcggcg ctgtcttttt aggagttact
      241 gtaggagtcg ggctagcggt aggagccgtt gccgtcgttt gaggagtcgc cacagttgct
      301 ttagttgccg gagcaagcgc aactggggta acggtagtct tttttgacgt atttgtattt
      361 ttagtagcat cgctatgtac gctcttctta tctgaggcgt tattaacttt tacataagca
      421 gcactcaaga taccgactac cagcacacct attacagcta gcaaaataat atgagtaaag
      481 ccatcttgtt tgttttttat tttcatcatg atgtatttat tataaggtat ccttgttaat
      541 gcgcatatgc ttactgctaa gcctaaggat gactcaagtc aaaatcctat tcgtaagttg
      601 gaatatattg agccgagact gaatgcgttg gggatacctt aacggtatat aaaggtttat
      661 attggtattc atatgtatta tactaaccct aggaggacta catatgtcaa caactcaagc
      721 tttcaagtta gatgatgaaa cgaacgaacg attaaaggca ctcgcgcagc agcgtgacag
      781 gtcggcacat tggctactgc gtgaagcatt acagcgatac ctgaccgagg aagaacgcta
      841 cgagcgagaa aaggcagaag atttagctga gtacaaggat tacctattga ccggcaaggc
      901 cgtggatagc gaaaccgtaa cgtcttggct taacgagctt gcaagcggca agaaaacgac
      961 atggccaaag caaaagtagt ttggcgtgag cgcgcacttg aagatattga ccgtttgtat
     1021 gacttcctat tcaaaaagaa tgaagaagca gctacaaaag tggcccaggt aatcttacga
     1081 ggcagtttac tactcgaaga atcaccgcga ctagggcgcc caatggcgga cggctcagaa
     1141 agacgggaac tatttattcc atttggagcc ggtttttatg tgatacgcta ctttctagcg
     1201 gatgatattg tcgtcattgt gcgtgtttgg catgggcgcg aagatagaaa agcggattaa
     1261 ccctacacga gagataaata cagtttcgaa aaaatattct tgttacgaaa tggtgacctc
     1321 acggagaatc gaactccggt tgctgggatg aaaacccagt gtcctagccg ctagacgatg
     1381 aggccgtgag cgaaggaagg tgtagatttt atgcttgcat aaaattatct tccgagcgaa
     1441 cggtttcata tcgaagatat gaggccaaac gttgttgtct tacttgtgca ttctatagta
     1501 ggaaaacgac cccgaaatta taccacaaca gatacaaaaa gaaaatcccc gtctgcaacg
     1561 gggatttagg agcggtcagt cacgattact ctctcaaact ctgcgtcgta gcctaccata
     1621 ctctcataca ttaagctagt gtttatccac agctttatat ttactgcgca ctggaggcgt
     1681 tcaagatcac tgtgcgaagg taacgaccgt agcacgagct taactagatg cgtatacctg
     1741 cgaaaaatcc ccactctcat acgtatacgt gggtggggat ttcgcagttc tttcgagcgg
     1801 tcagtcttgc tctttgtaag ctacagcgct acagactagc ggcgagcaag cttacggttt
     1861 agtagcaacc gatagccaat agtacccgcg acaactacgc tagcaaagat agcgattgtg
     1921 tcacctgcac ccgtatttgg caactgctgc ggttggggag ttgttggcgg ggtggtaggc
     1981 ggagtaactg gtggcgtcac tggaggagta acgggtggag tggttgtctt gaaggtaact
     2041 gtctgcgtac aaccttggct ggaagcagac ttagttgcac cgttcactcc aaagttagca
     2101 gtagcaacaa ttgtgtagct gccatctgca gtgtacgtgt gtggctgacc aataagtgta
     2161 gttagcgggc tggtcttggt cttgtcgccc cagtcgatga cagcgttctg gaaagtagca
     2221 ccacccgtct gagtggtctt aaactggtca acaactacct tgcgggtagc gttgtcggct
     2281 accacgtgca gcaagtcaca gctgtaggcg ggagtaaccg gtggcggagt tgtaggcatg
     2341 tgtactttta ctttaacggt cacgtagcca gagtaggcaa aacagccact caggtcacct
     2401 agtgaagtgc cgttggcgcc agcgatgtca tcgctaagtg taacggtagt ctgatgtaca
     2461 ccatcagcag catggctcca ctgtgcagaa cctttcacat attccagcgt aaaggcgcta
     2521 tcggcataga cactcatagt gtcgttaacc atagctggag tagcgttgct ggccgtaatg
     2581 aaaccaaaga tgttggtgtc tgactggctg gcagtcggca caagcagcct aaaattaaca
     2641 ttcttagcga cagttttgtc ttcgccgccc ataaggctgg cgtcggcatt gttgtgaata
     2701 taagcacgaa cggtaacaac gtcaccgtct tttacttgca aacgatcctg gacattggca
     2761 ccgttaaggc tgccagccag gaagggacgc tcatcaccga caactgggtt gttggtgaat
     2821 gagtcgaacg taacgtggtc tgcaccggta cagttagtag gggtagtaca agtgtaggtt
     2881 ggacgatcca aagggtagta gttcgcgtga gcgacaccgc taacaccaaa tgccattacc
     2941 gttacaacgg cgctggccaa aatgcgagtt atcttcatga tatgcatttc ctttcaggtt
     3001 aaaaacaagg gaaatatagt acagttagta aagaaaatcg gttgccaaac tgttaaagcg
     3061 cgtcagcagg catgtaattt ccacctcaca tggtactaat ggtttattta attgtagcat
     3121 aattattatg tactgtcaat tttagtgtcc cgttagcctg ctgtcggttc caaattgtta
     3181 agtttgcttg accgctgtag tggtcacttt tataggcaaa tcagatcgtt acaaaacata
     3241 taagggttgt gcaggtgaag tgggagttga tttttgaagg tgccggctgg atgccgtcag
     3301 atcttcttgg tcaacaacca ctccaccatg caggtgtaac gctttttgtg cttggcacaa
     3361 ccctttgcta tcgcttcgcg atcggtggaa ccagccggcc cggacctttt tcatgactgg
     3421 atgccatgaa ttacactccg aatggagcgc cggtccgggc tgcagacggc acgactaggg
     3481 gcatctgccc gtcgtcgggt cgggtgcggc gcagccggag ctgctcggat ccgccggtgg
     3541 cggattcgtg ccgtgacccg aagggggctg cgaagtcggc tggctgttgc cgttcttgtg
     3601 gcagtcgttc ggcggagttc cgatgtaccc cacgccgtgc tcgaactggt cgcaggtcag
     3661 cgacgtcgtg gtgtgcgtcg tcgacggaac cgtagtcggg tgcgtggtgg gcgccgtcgt
     3721 cggcacggtg gtcggcgtcg aactcgtcgg cgtcgggacc ggcgtggtcg ggtgcgggtg
     3781 cgtcggcgcc gtggtcggtc cccggatggg ggtggccggc tggtacggct gagacggctg
     3841 accgccgcag accagacgca ggtggaccac caggccgctc ttcatggtga tgtccagaga
     3901 ctcaccgagc gactcgtcgg acacgctgtt gaacgtgagc ttcggcgccg gcttggccgg
     3961 attggccttg ttcacgtgca tctccagcgt gtagaagcct cgcacgtcgt atgtgacgag
     4021 cttgcagctc accgcgtcac cgtacagggt ctccagcgcg ccggcccagg ccgtgtcgtc
     4081 agcggcgtac ttgctggccg tcgcgtcgat gtcgttgaac ttcgactcga gggccttagg
     4141 cacgttcgcc gtgtctttgg gcaactgcga agcagtgacc acaatggccg caaactgcgg
     4201 gtcgtggatc agcttgtcgc cggcccaggt gatgacctcg gactgcttcg tggacgtggt
     4261 accgtctccg aactcgccct cgaccgtctg gtcgtacgcg tcgtacttca cgccgttcgg
     4321 cgcgatgtcg gcgctggccg gcaccggctt ggtggcgcag ctggtcgcgg tgaccgaagc
     4381 cggagcgctg agagcgcccg tagccgcggt gacgcagagg gcgaccagca cgacgataac
     4441 gccgatgatc gcgatcaccg gtccggcgat cttactcttg ttgcgggaca tgctacctct
     4501 cctgcaagac aggccctgat gggcgtggtg ggtagaacac taccaacgcc catcgggcaa
     4561 ttggctacta gtgacgcggg ccgtcagtcg tggcctgctg gacgggcttc tccggcttgg
     4621 ccgggatggc ctggaccagc agcaagacca gggcggacat gaccagggcg atgcccaggc
     4681 cgatccaacc ccagggagcg gcgctcatgc cggtctgcgt gtgagcctgg ttgcccatac
     4741 cgtagacctt gctggcgcgg tggccgatcc actggcagac gaaccaggtc aggataccga
     4801 cgacgatcgg gatccagagg agtcccttcc gcacgcggcc gatggcgtcg atcttcttgt
     4861 cctgcgcctt gagcgcgtcc accgcgacga cgccgagggc gtgagccctg gtgatcctct
     4921 cgtcgtggtc ggcaaaccgc cggtccgtct cggtg
//
LOCUS       DATNGP010000163         4853 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_2884716, whole genome shotgun sequence.
ACCESSION   DATNGP010000163 DATNGP010000000
VERSION     DATNGP010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4853
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2884716"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1164
                     /locus_tag="VN778_04195"
     CDS             <1..1164
                     /locus_tag="VN778_04195"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXR50198.1"
                     /translation="HGKHVFNPAAFAAAFLSTWGFWPATWWIGSSKFWPFTLLLGLLV
                     VRKMRRFSLFASFVVISIVLQTALLLHGHQPLATSMKQAVLASPLIFLSTIMLIEPAT
                     MPPRRSLQIVFAALVATLYVTGWKIGPLIIVPEVALLFGNLFAYLVSPKFRVQLVLKE
                     VHKISGRIYDYVFMPDVHFQFLPGQYMEWTLAGVPYDSRGNRRTFTIASSPTEEEVHL
                     GLKYYEPSSTYKATFYELKPGDIVYASQLAGNFTLNGNEKKKLVFIAGGIGITPFRSM
                     VKYLTDNNVSCDVVLLYLVSDPQEFAYAHEFKQAAKIGVKTIPVITNPSKHAPGAVSA
                     KLSPHLLSQAIPDYAERVFYISGSNHFVDVAKSQLRSLDVPRRHIKTDHFSGY"
     gene            1233..1607
                     /locus_tag="VN778_04200"
     CDS             1233..1607
                     /locus_tag="VN778_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020458971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HXR50199.1"
                     /translation="MITFRLNTHSGVPPYLQIVQQVRQALQVGILQPGDQLPTVKEVV
                     AHIAINPNTVAKAYRELESLGITEGRTGSGTFVKQRPAGPSPAIQEALSQDLEGWIAK
                     ARQAGLDEEGIEALVRVTLHKK"
     gene            1622..2497
                     /locus_tag="VN778_04205"
     CDS             1622..2497
                     /locus_tag="VN778_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013734146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXR50200.1"
                     /translation="MGKAAIQTKELGKRYGSFWALENCNISVPEGSVSALVGPNGAGK
                     STLLKLLVGLSSSSTGEGEVLGKAPVQHEDFLTDIGYLAQEIPLYKSFNAADHITMGQ
                     HLNKNFDKKFIVKRLKELGIPLDRPVGKLSGGQRAQVALALALAKKPKLLLLDEPVAA
                     LDPLARHDFLASLSQAVADGGLTVVMSSHLLADLERVSDHVIVLASGKTQLCDDIETV
                     LQDHKLIVGPREHAKNLGDGISVIHETHTVKQSTLLVRLSHGAKLPNHWQQHEPNIEE
                     IILAYMGQAKEGGAV"
     gene            2494..3534
                     /locus_tag="VN778_04210"
     CDS             2494..3534
                     /locus_tag="VN778_04210"
                     /inference="COORDINATES: protein motif:HMM:NF024141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HXR50201.1"
                     /translation="MNWFVWRQHRKQFLSFGVLLALFAALLIPTGIHYWHTYQQALTN
                     CAQHPALYKCDDLTNTLFSSQADGFIRIAEVLGTFGLPLLVGLFVGAPLVAREYEEGT
                     NKLAWTQSVSRRKWLTTKVVWVLGFALLYGITLTILATWWSRTLNTVAHNRFVQGHFE
                     TQGLMPVAYSVFFTALGFAVGAWFRKTILAFAITFGVFVLCMASFANWVRPHYMTPVT
                     VTAAMGPNSLDGKIPTDAWILQRNIVDKNGKVLNGDLFREAPPQCQKILQQAEVQGNG
                     HGGIRVKAVPNPNGGDPIDDCLNKAGFHQIAKYQPSYRYWDFQRIEASIYLGMTALAV
                     AAKYWLVLKRDA"
     gene            complement(3548..4348)
                     /locus_tag="VN778_04215"
     CDS             complement(3548..4348)
                     /locus_tag="VN778_04215"
                     /inference="COORDINATES: protein motif:HMM:NF014453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HXR50202.1"
                     /translation="MSEGIFLLGWLIAAQMLLQASIAANISFERSRVINRLTGEKYHN
                     SARKEVINRMAGLACAMITAPLAGTACVGLMAENGTTDVLFKQERLGSDGQLVTIRKF
                     RTIYRDVELTIPFSGPDDPRTKGLARALRRSGLDKWPQLLDVIAGKEHFFGLRSINEE
                     HAQSYRKLLGIRKDLVDRRLEAGQWCSGHVSPTNVTDHLYPEHTREVIINRFEDDLHY
                     AHHGTLHTDLKTFLRMPGILALAGLGYRRQPPEAEPVLSAPSALAIPS"
     gene            4416..>4853
                     /locus_tag="VN778_04220"
     CDS             4416..>4853
                     /locus_tag="VN778_04220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HXR50203.1"
                     /translation="MQFSDFGKKMSGEAGILSLMDDMGKALAGNRPLAMFGGGNPAHI
                     PAVTAAFKQSLHDILADENRLLAMLGNYDTPQGNEAFIATVRDFFNRRYSWNITAENI
                     AITPGSQAGFFMLFNLLAGRSPEGGKKILFPLVPEYIGYVDQAL"
BASE COUNT         1163 a         1273 c         1215 g         1202 t
ORIGIN      
        1 cacggtaagc acgttttcaa cccggcagcc ttcgctgcag ccttcctgag cacgtgggga
       61 ttttggccag ccacctggtg gattgggagc tctaagtttt ggccttttac actgctttta
      121 ggcctgcttg tcgtcagaaa aatgaggcgc ttttcacttt tcgcttcgtt tgtagtaatc
      181 agtatcgtac tgcagactgc gttactgctt cacggtcatc aaccattggc aaccagcatg
      241 aagcaagctg tgctggcttc gcctttgatc tttctgtcga ctatcatgct aattgaaccg
      301 gcaaccatgc cgccgcgccg cagcttgcaa attgtttttg ccgcactggt tgccactctc
      361 tatgtgaccg gttggaaaat tggaccactc attattgttc ccgaagttgc cttgttgttt
      421 ggcaacttat tcgcctatct ggtatcaccg aaattccgcg tgcagctggt gctaaaagaa
      481 gtgcataaaa tctctgggcg aatatacgat tacgttttta tgccggacgt acacttccag
      541 tttttgccag ggcagtacat ggaatggacg ctagccggcg ttccgtatga cagccggggc
      601 aaccgccgca ctttcacaat agcttcctcg cccacggagg aggaagtgca tttaggacta
      661 aaatactacg agccttcaag cacctacaaa gcaacatttt atgaattgaa acctggcgac
      721 atcgtctatg ccagccaatt agccggtaat tttaccctaa acggcaatga aaagaagaaa
      781 ttggtcttta tcgcgggcgg tatcggcatt accccgtttc gcagtatggt caagtatctg
      841 accgataata atgtcagttg tgacgttgtt ttgctgtatt tggtttctga cccgcaagag
      901 tttgcttacg ctcatgagtt caaacaagcg gcaaaaatcg gcgtcaaaac catcccggta
      961 atcaccaatc ctagtaagca cgcaccaggg gccgtatccg ccaaattaag tccacatctt
     1021 ttatctcaag ccataccgga ctatgctgag cgtgttttct acatttctgg ctcgaaccat
     1081 ttcgtggatg ttgcgaaatc ccaattacgc agcctggacg tgccacgcag gcacattaaa
     1141 accgatcatt tttcaggcta ttaagggtat ttcttgacgt ctaattattt ttgcttatac
     1201 ttttactagg aaactagtat aatagtaata ttgtgattac tttccgcctt aacacacact
     1261 ccggcgtgcc gccctatctg caaatcgtgc agcaggttag gcaggcgctg caggtcggaa
     1321 tactacagcc gggtgatcag ctgccaactg tcaaggaagt tgtcgcccat atcgcgatta
     1381 atccaaatac agtggccaaa gcttaccgcg aactggaaag cctaggcatc accgaaggcc
     1441 gtaccggtag cggcaccttt gtgaagcagc ggccagccgg cccgtcgccg gccatccaag
     1501 aagctttgtc gcaagatttg gaaggctgga tagccaaggc ccgccaggcg gggctggatg
     1561 aagaaggtat cgaagcgctc gtgcgcgtta cattacacaa gaaataggag ggtatctcag
     1621 aatggggaaa gcggcaatac aaactaaaga gctcggcaag cgatacggct cattctgggc
     1681 gctcgagaat tgcaatatat ctgtgccaga aggcagtgtc tcagcgcttg tggggccaaa
     1741 tggtgccggc aaatctacat tattgaaatt gctcgtcggc ctgagcagct ccagcaccgg
     1801 cgagggcgaa gtgctgggta aggcaccggt gcagcatgaa gattttttga cagacatagg
     1861 ctacctggcg caggaaattc cgttgtataa atcatttaat gccgccgacc atatcacaat
     1921 gggtcagcat ctcaacaaga actttgataa gaaatttatt gtaaagcgcc tgaaagaact
     1981 cggcattccc ctcgaccggc cggtgggcaa actctccggc ggccagcgcg cccaagttgc
     2041 tctggcgctc gctctagcca agaagccaaa gctcttgctg cttgatgagc cagtcgctgc
     2101 gctcgacccc ctggcccggc acgacttcct cgcttccctt tcccaagctg tcgccgatgg
     2161 cggattgacg gttgtaatgt catcacacct attagctgac ctggagcgag tgagcgacca
     2221 cgttatcgtt cttgcttccg gcaaaacaca gttatgcgac gatatcgaaa cagtgctgca
     2281 ggaccacaag ctaattgttg gaccacgtga acatgccaag aatctaggcg atggcataag
     2341 cgtcatccac gaaacgcaca ccgtcaagca atcaactctt ctcgtgcgcc taagtcacgg
     2401 cgccaagctg cccaaccact ggcagcaaca cgagccgaac atcgaggaga tcattcttgc
     2461 ctacatgggc caagccaaag aaggaggcgc cgtatgaatt ggtttgtctg gcggcaacac
     2521 cgcaaacaat ttttaagctt tggggtattg cttgctttat tcgcagcgtt acttattccc
     2581 accggtatac attattggca cacttaccag caagcactga ccaattgcgc gcagcatccc
     2641 gcgctgtaca aatgtgacga cctcaccaac accttgttca gcagccaagc cgatgggttt
     2701 atcaggatcg ccgaggtctt aggtaccttt ggcttaccac tgctcgttgg actatttgtc
     2761 ggcgcacctt tagtggcacg tgagtacgaa gaaggaacta acaaattagc ttggactcag
     2821 agcgtgtcca ggcgcaaatg gttgactacc aaagtagtgt gggtactcgg attcgctttg
     2881 ctatacggaa ttaccctcac aattctggcg acgtggtggt cacgcaccct gaatacggta
     2941 gctcataacc gctttgtaca aggccatttt gaaacacaag gcctgatgcc agttgcatat
     3001 agtgtcttct ttaccgccct aggttttgcg gtaggtgcgt ggttccgcaa gactattttg
     3061 gcttttgcga taacctttgg tgtatttgtg ctatgcatgg ccagtttcgc caactgggtc
     3121 cggcctcact atatgacgcc agttactgtt actgctgcaa tggggccgaa tagcctcgat
     3181 ggcaaaattc cgacagacgc ttggatactg cagcgcaata ttgtcgataa aaacggtaag
     3241 gttcttaatg gcgatctttt ccgggaggca cccccgcagt gccagaaaat tcttcaacag
     3301 gcagaggtac agggtaacgg ccacggcgga atccgggtaa aagccgtccc gaatcctaat
     3361 ggtggtgatc ccattgacga ctgtttgaac aaggccggct tccaccaaat tgccaaatac
     3421 cagccatcct accgctattg ggatttccaa cgcattgaag ccagcattta ccttggcatg
     3481 actgccttag cagtagccgc taagtattgg ctcgtgctca agcgtgacgc ctagatggtc
     3541 atttcggcta gctagggatg gccagcgccg aaggagctga gagtaccggt tcggcctccg
     3601 gcggctgccg cctgtatccc aagccggcca gcgccaagat ccccggcatc cgcaaaaatg
     3661 ttttgagatc agtatggagc gtgccgtggt gtgcgtagtg aagatcgtcc tcaaaacgat
     3721 tgatgattac ttctctggtg tgctcaggat acaaatgatc agttacattc gtcgggctga
     3781 catggccgct gcaccactgc ccggcctcca gccggcggtc caccaggtct tttcgaattc
     3841 ccagcagctt tcggtaagat tgcgcatgct cctcgttaat cgatcgcagt ccaaaaaagt
     3901 gctctttgcc ggctatgacg tcgagcagtt gtggccattt gtcaaggccg ctgcgccgga
     3961 gcgctcgggc aaggcctttg gttctcggat cgtctggccc gctaaaaggg atggttagtt
     4021 caacatcacg ataaatggta cggaatttgc gtatggtcac aagctgacca tcgctgccca
     4081 gcctttcttg cttgaacagc acgtcagtcg ttccgttttc ggccattagt cccacgcagg
     4141 cagtgccggc tagtggcgcg gttatcatgg cgcaagctag accagccatc cgattgatga
     4201 cttctttcct ggcgctatta tgatatttct cacctgtaag acggttaata accctggacc
     4261 tctcaaaact aatattagcc gcgatgctcg cctgtaacag catttgcgcc gcaattaacc
     4321 agcctaaaag aaaaatgcct tctgacaaat taagtatttt acaaaccccc tgtgcgcctt
     4381 gtatagtgct gcttattata agggtactaa aagacgtgca gttttcagat tttggcaaaa
     4441 aaatgagcgg cgaggctggc atactcagct taatggatga tatgggtaag gcgctggccg
     4501 gcaatcgtcc gctggccatg ttcggcggcg gcaatcctgc ccacattccg gctgtgaccg
     4561 ctgcatttaa gcaatccctc cacgacatac tggctgacga aaaccgactg ctcgcgatgc
     4621 ttggcaacta cgatacgccg cagggcaacg aagcctttat cgccaccgtt cgcgatttct
     4681 ttaaccgccg gtacagctgg aacattacag ccgaaaatat tgctatcact cctggcagcc
     4741 aagccggctt tttcatgctc ttcaatttgt tggcgggcag aagtcctgag ggcgggaaga
     4801 aaattttgtt tccgctcgta ccagaatata tcggttacgt ggatcaggcg ctg
//
LOCUS       DATNGP010000164         2094 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_1407195, whole genome shotgun sequence.
ACCESSION   DATNGP010000164 DATNGP010000000
VERSION     DATNGP010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1407195"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /locus_tag="VN778_04225"
     CDS             <1..275
                     /locus_tag="VN778_04225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50204.1"
                     /translation="ACTRRHVRKLGPITSTVVGSTVILRQQHQPSRRIYSARTAERAV
                     AGLLEVDVGVDARGRTYDPVAATREALAGAQAIRDADVVGRVAEDR"
     gene            318..617
                     /locus_tag="VN778_04230"
     CDS             318..617
                     /locus_tag="VN778_04230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50205.1"
                     /translation="MREPQGPHEHAVDLDCHDDGLAGVNTGGVVGDARDLHGQRAVAS
                     YDVRVDGRRCAAAEVPRKAVADRDTTPVYVLWGVFSPRHTAAERAQQCRPLATNR"
     gene            complement(1177..1827)
                     /locus_tag="VN778_04235"
     CDS             complement(1177..1827)
                     /locus_tag="VN778_04235"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXR50206.1"
                     /translation="MADKKLAIITGASRGLGLALVQKFAASGWQVVGTGRSERPADLP
                     AEVAYKQFDASNAAECEAFWQQLKADYPEATVCLVNNAGSYVSGSLTETKSEDYLQQM
                     QSCYFSSVYMTRGLALAFPQARIVNIISNSALAAHKSNSAYGPAKAAQMHFFQSLQEE
                     FAPKKYQITNLYPSDIATHGDNPEAISAADLASFVYQQAENTSTYYLRDVTVYPVV"
     gene            complement(1835..>2094)
                     /locus_tag="VN778_04240"
     CDS             complement(1835..>2094)
                     /locus_tag="VN778_04240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50207.1"
                     /translation="GQRFELHGEATPDKQFYPLDGAVAGFEVPDMDAAIAWAEKNNLE
                     MFEEGTGSGKGGTRWAHFCGPDGNIYEFVYHPSILEGGKPE"
BASE COUNT          469 a          599 c          534 g          492 t
ORIGIN      
        1 ctgcttgtac ccgccgccac gttcgcaagc tcgggcccat cacaagtact gttgttggca
       61 gtaccgtaat tctgcgacag cagcaccagc ccagtagacg aatctacagt gcccgtactg
      121 ccgaacgtgc cgtagcaggc ctgcttgaag tcgacgtggg tgtggatgcc cgaggtcgta
      181 cttacgatcc agtagctgct acacgcgaag cccttgcagg tgcccaggct attcgtgatg
      241 ctgacgtcgt cggccgtgta gccgaggacc gttgatgctg tgactgtgtc attcgcggca
      301 acctgcacat tcacgaggtg cgcgaaccgc agggtcccca cgagcacgcc gtcgaccttg
      361 actgccacga tgacggcctt gccggtgtca acactggtgg tgttgtcggc gacgctcgtg
      421 acctgcatgg tcaacgtgcc gtcgccagtt acgacgtgcg ggtagacggc cgcagatgcg
      481 ccgccgcgga ggtcccacga aaagccgtag ccgatcgcga caccacccct gtctacgtgc
      541 tctggggcgt tttctcccca agacacactg ccgctgaacg ggctcagcaa tgccgcccgc
      601 ttgccaccaa tcggtgacgc cgacgccgac gtagccgtga cgaccactcc gaccagcgag
      661 gcagcgagca agctcaccac cacaactagc gttttgagag cttgtgctct ccttgcaaac
      721 caaccgttca cttgatgatc ttctctgtga gaaaaactgg tgaaactgcg ctgttaagga
      781 accgcctcaa tgggtttgag gatttcccgt gtcttaccta tacgtttgta ctaagagaca
      841 gttatttata acataacgaa gcaataaggc aaaataaccg taggggcgca tacacaccca
      901 caaaacaagc cgtttccaca tggcgcattt cgtctttttg gtacagccaa aaagccaagg
      961 gtggagtatg tccagtgggc atacccgttc gactccccgg gaatgaccac gaaaaagccc
     1021 cagctttcgc tgaggcgttt acgtggtgaa cctcattgag tcgaactgga acctaatctg
     1081 tgaagagata ctacgctggt gcaaattact ggcggggcaa caccggtcat ttgaactccc
     1141 tgctgcgact agaacctatg gcttagtata gccagtctaa acgactggat ataccgtaac
     1201 atccctcaaa tagtaggtgc tggtattttc tgcctgttgg tacacaaaac ttgctagatc
     1261 ggcggcgctg atggcttcgg gattgtcgcc atgcgttgca atatcgctag ggtatagatt
     1321 ggtgatctgg tattttttcg gcgcgaactc ttcttgcaac gactggaaga agtgcatttg
     1381 tgcagcctta gcgggaccgt aagcagaatt actcttgtgg gcggcgagag cgctatttga
     1441 aatgatgttc acgattctgg cttggggaaa cgcaagcgct aagccacgcg tcatgtatac
     1501 agacgaaaag tagcagctct gcatctgctg caagtagtcc tctgacttcg tttcggtgag
     1561 gctaccgctt acatagctac ccgcgttatt aaccaggcat acggttgctt ctgggtagtc
     1621 ggcctttagc tgttgccaaa acgcctcgca ctcagcagca ttactcgcat caaattgctt
     1681 atatgcaact tctgccggta agtcggctgg tcgctctgac cggcctgtgc cgacaacttg
     1741 ccagccactc gctgcaaact tttgcaccaa cgccaatcca agaccgcgac tagcccctgt
     1801 aattatcgcc aactttttgt ctgccatatc agtcctattc tggctttccg ccctctaaaa
     1861 ttgatggatg gtatacgaac tcgtatatgt ttccgtcggg tccacaaaaa tgcgcccaac
     1921 gcgtaccgcc cttgccgctg cccgtaccct cctcgaacat ttcgaggtta tttttttcgg
     1981 cccatgcaat tgcagcatcc atgtcaggaa cctcaaaccc ggcaacagca ccgtccagcg
     2041 gataaaactg cttatctggt gtcgcttcac catgtagctc aaagcgctgg cctt
//
LOCUS       DATNGP010000165         2537 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_2254067, whole genome shotgun sequence.
ACCESSION   DATNGP010000165 DATNGP010000000
VERSION     DATNGP010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2537
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2254067"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1007)
                     /locus_tag="VN778_04245"
     CDS             complement(<1..1007)
                     /locus_tag="VN778_04245"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50208.1"
                     /translation="MFEKLLSQLPYNPGLSHQLAFYARRMREEATIRRTGLVFIALAF
                     MVQFIAVLSPPQSTTAASNNDLINGGFSSAAEAARYCQNNTAGYGTILGNYGITCTEV
                     ANSPVVTVHSTDSNRKLYSMGRLPYGLAGETPVTIGGKTYYFRYLWSWDTHGASTYSA
                     LRIIARATGKTYYLLYTCGNLVSVGLPQAYSPPGATFTPVIPSVPRLSLTKTTIAGYP
                     AAGSTVAPGTTLGYRVNVNNTGNGTAHTVTLIDTIPSNTTFVSQSLNAGASVHSFDTS
                     TRTAKWVWATVPPETGNYSVDLKVTISAATPNGTQICNMARVDSPDTPASNSNQVCVT
                     V"
     gene            1132..1443
                     /locus_tag="VN778_04250"
     CDS             1132..1443
                     /locus_tag="VN778_04250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50209.1"
                     /translation="MERQDIKKLWPSVGCSIRKDEVTFHGTGREAELIVAGLKVLAGQ
                     IAIRDAVSEEPVSAVDSRPYSDYKRDQGRGVPLKGEVDKLLQDIREPNFVPAPEAAAV
                     S"
     gene            1554..>2537
                     /locus_tag="VN778_04255"
     CDS             1554..>2537
                     /locus_tag="VN778_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006317200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXR50210.1"
                     /translation="MAKDKQAGYSAEQITVLEGLEPVRKRPGMYIGGTGVEGLHHLVW
                     EIIDNGIDEALAGHATEVVVTLLADGGVRVFDNGRGIPTDIHPKTGKSTVETVLTVLH
                     AGGKFGSGGYKVSGGLHGVGVSVVNALSTKLHVKVYRSGKIWEQDYERGAPQGDLKAT
                     GKTDQTGTEITFYADETIFETTKINYETVLDRLRHAAYLTKGIHTSITDERSGARYGF
                     YFEGGIQSYVKHLNIGKEVVDDDIFYVDKMVQEAQIEISLQYTDAYTEVIRAFANNVL
                     NPDGGTHLTGFRAALTRVINDYARKNGLLKEKEENLTGEDTREGLTAIILVK"
BASE COUNT          613 a          564 c          791 g          569 t
ORIGIN      
        1 ttgacggtga cgcagacctg gttggagttg ctggccgggg tatccgggga gtccacccgc
       61 gccatgttgc agatctgggt accattcggg gtggcggcac tgatcgttac tttcagatcg
      121 acagagtaat tgccggtttc gggcgggacg gtggcccaga cccatttggc ggtgcgagtg
      181 ctggtatcga agctgtgtac actggcaccc gcgttgaggc tttgggagac gaaagttgtg
      241 ttacttggaa tggtgtcaat aagcgtgaca gtgtgagcgg tgccgttacc ggtgttattg
      301 acattgacgc ggtagccgag cgttgtgccg ggagcgaccg tactgccagc ggcggggtag
      361 ccggcgatag ttgttttggt gaggctgaga cgtggtaccg atggtatcac gggagtgaat
      421 gtggcgccgg gtgggctgta ggcctggggt aagccgacac tgactaggtt gccgcaagtg
      481 tacagcaagt aatacgtttt gcccgtggcg cgggctatga tccgcaaggc tgaatacgtt
      541 gaggcgccgt gggtgtccca gctccagaga tagcggaagt aatacgtttt gccgccaatg
      601 gttactggtg tttcaccggc caagccgtac ggcaggcggc ccatggagta gagcttgcga
      661 ttgctgtcgg tggagtggac ggtgaccact ggggagttgg cgacttcggt gcaggtgata
      721 ccgtaattgc ccaagatagt gccgtaaccg gcggtattgt tttggcaata acgggcggcc
      781 tcggcggcgg agctgaagcc accatttatg aggtcgttat tggaagcagc tgtcgtcgac
      841 tgcggcggcg aaagcacggc aataaactgc accatgaagg ctaaggcgat gaaaaccaga
      901 cctgtacgcc ggatggtggc ttcttcgcgc atgcgccgcg cataaaaagc cagctggtgg
      961 ctcaaaccag ggttgtaggg cagttgcgaa aggagttttt caaacattgt ttttatttga
     1021 acgtttttac atccttattt aatcacaagc gacatactaa cagcaagatg taaatgctaa
     1081 tgcttgctga gtgggagccg ggcaattatg ataacgacag gaggatattt tatggagcga
     1141 caagatatta aaaagctgtg gccgtcggtc ggttgttcaa tcagaaagga tgaagttacc
     1201 tttcacggca ctggtagaga ggcagaatta atcgttgctg gcttaaaggt tttagccggc
     1261 caaatagcta ttcgtgacgc agtctcagag gagccggtaa gcgcagtgga tagcaggcct
     1321 tacagtgatt ataagcgtga ccaaggtcgg ggcgtgcctt tgaagggcga ggtggataaa
     1381 cttctccagg atatccgtga gccgaatttt gttcccgctc ctgaagcagc agcagtttct
     1441 taaacagaga ataaatccgc aactttggtt gttaaccggg gcgaattagg ctatactata
     1501 aggagtggga aaaagtaaga ctcgtagcag tcattaactg aggtgtatta ctagtggcta
     1561 aagacaaaca agctggctat tcagctgagc aaatcacagt attagagggg ttggagccgg
     1621 tccgtaagcg tccgggtatg tatatcggcg ggacaggcgt cgaaggcttg caccacttgg
     1681 tatgggaaat tattgataac ggcattgacg aggcgctagc cggtcatgcc acagaagtgg
     1741 tcgtgacact actggctgat ggcggcgtcc gggtcttcga caatggtcgc ggcatcccga
     1801 ctgacatcca tccgaaaaca ggcaagagta cagttgagac ggtattgaca gtgctgcacg
     1861 ctggcggcaa gttcggcagt ggtggctata aggtgtctgg tggtttgcac ggcgtcggag
     1921 tcagcgtggt taacgcgctg tcgaccaagc tgcacgtcaa ggtctatcgg agtggcaaga
     1981 tctgggagca agattacgaa cgcggtgcac cgcagggcga tctaaaggcg accggcaaga
     2041 ccgaccaaac aggcaccgaa atcacttttt acgccgatga aacgattttc gaaacaacca
     2101 agatcaatta tgagacagtg ctggaccgct tacgccacgc cgcgtatctg accaagggca
     2161 ttcataccag cattaccgat gagcgcagcg gcgctcgcta cggcttttac ttcgagggag
     2221 ggatccagag ctacgtcaaa caccttaata tcggcaaaga agtagtcgac gacgacattt
     2281 tttacgtcga caaaatggtg caggaggcgc agatcgagat ctcgctgcag tacactgatg
     2341 cgtacaccga ggtcatacgg gctttcgcca ataacgtgct caatccggat ggcggcacgc
     2401 acttaactgg ttttcgggcg gcgctgacac gcgtcattaa tgattatgct cgtaagaatg
     2461 gcttgctgaa ggaaaaggaa gagaatctga ccggcgagga tactcgcgaa ggcctaaccg
     2521 ccatcatttt agtcaaa
//
LOCUS       DATNGP010000166         3477 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_473817, whole genome shotgun sequence.
ACCESSION   DATNGP010000166 DATNGP010000000
VERSION     DATNGP010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3477
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_473817"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..573)
                     /locus_tag="VN778_04260"
     CDS             complement(<1..573)
                     /locus_tag="VN778_04260"
                     /inference="COORDINATES: protein motif:HMM:NF013179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HXR50211.1"
                     /translation="MDHLQQLKHQVRQYVFIVVLLENLALVACFWAIEQFSGLPTLIN
                     ALISLLLSLVLGVAVMKIVSDYVVVPTQALWQAILHLSPTEHGIPAPKPEELRVGREL
                     VTSLTAQIYQLASVAEHAAEEGHLQSSDVRRNFVAQNLPLPLFVLDPSEIVTFANEAA
                     AQYIGIAVEDLIGKNIYMVLDMSFPSEDTFD"
     gene            complement(592..1869)
                     /locus_tag="VN778_04265"
     CDS             complement(592..1869)
                     /locus_tag="VN778_04265"
                     /inference="COORDINATES: protein motif:HMM:NF022702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3048 domain-containing protein"
                     /protein_id="HXR50212.1"
                     /translation="MDSDFRPIKRPVRPSPSDTAQTSKPPLPNKSFHTPEEVAAVDDI
                     TTPAPLAELPAMTDEHLSPKKTTSWRDKFRLQWPPGQKEWLASAIVVVVCGSIIGLVL
                     THTGPKPVASAIKVTKPKPVPKPTTVPSTLTGLPVDPSVNQRPVTGVMIENTTFARPQ
                     SGLGQAGVVFEAIAEGGVTRFLALFQDTAPSNIGPVRSARPYYIQWAMGFDAAYAHVG
                     GSQDGLADIKAWGVKDLDEFANAGAYHRISSRAAPHNMYTSIAALNQLETSKGYTTSN
                     FTGFPRKPEAPSKTPNARSINLTLSGPVYNAHYDYNAATNSYNRSEGGAPHIDANTNQ
                     QISPKVVVAMVVPMTRGALDSSGAYYSNYASVGSGAVYVFQDGLVTTGQWSKPSNTAQ
                     ITLTDASGQPLKLNPGQTWITAVIANNDVSYSP"
     gene            complement(1917..3290)
                     /locus_tag="VN778_04270"
     CDS             complement(1917..3290)
                     /locus_tag="VN778_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HXR50213.1"
                     /translation="MTKQKDILQRSAEAPAEILDLTVVATQKIATALPSNDLLRKTED
                     YWHMLGPGLTTGASDDDPSGIATYSQTGAQYGFQLLWLSLWTFPLSAVVQEMCARIGL
                     VTGRGLAGNIRAYFPKPILYVCTTLLFAANALNIGADIGAMAKAVQLLNPRLDFSMLV
                     IFITLLSLLIQIYTPYVRYARYLKWLAMVLFSYVLSAILAHLNWHTVFKHAFLPSVTF
                     SKDQLLLVCAILGTTISPYLFFWQTSQEVEEQILQGKTTIEERRTAADPADMKSMRID
                     VWSGMFLSELVMFFIIAACGSILFTHGITNIQTSAQAAEALRPFAGNATYFLFAAGII
                     GTGLLAIPVLAGSSSYALSESFRWREGLYRNLKQAYAFYGIIIISMFVGLAINFIGLD
                     PIKALIYAAVGNGLVAPIILLLIILISSNHKIMGHWTNKPWVTALGWLITLLMAVSGI
                     AAIYSLF"
BASE COUNT          815 a          831 c         1002 g          829 t
ORIGIN      
        1 atcgaaggtg tcttcgctgg ggaatgacat gtcaagcacc atgtagatgt ttttgccaat
       61 caagtcttcc actgcgatgc caatatattg agcggccgct tcgttggcga atgtgacgat
      121 ttcgctcgga tcgagtacga aaagcggcag cggcaaattc tgggctacaa agttacgccg
      181 aacatccgag ctttgtaggt gtccttcttc ggccgcatgc tcggcgacgc tagctagctg
      241 gtatatctga gccgtcagac tggttaccag ctctcgtccc acgcggagct cttcgggctt
      301 tggcgctggg atgccgtgct cggttgggct gagatgaaga atggcctgcc acaaggcttg
      361 ggtcggcacg acaacgtagt cgctgacaat cttcataact gctaccccta ggaccaagct
      421 gagcaggagg ctaattagtg cattgattag ggtgggcagg cccgagaact gctctatggc
      481 ccaaaaacaa gcgacgagag ccaggttttc caggagcacc acaatgaaaa cgtattgacg
      541 gacttggtgt ttgagttgct gcaggtggtc catcggctcc gtcgcttctg cttaagggga
      601 gtaactaaca tcgttattag ctataaccgc ggtaatccaa gtttgcccgg ggttaagttt
      661 cagcggttga ccgctggcat ctgtaagcgt gatctgggcg gtgttgcttg gcttgctcca
      721 ctggccagtt gtaaccagac catcttgaaa aacatatacg gcgccactgc caactgaagc
      781 gtaattagaa taataagcac cgctgctatc gagggcgccc cgcgtcattg gtacaaccat
      841 ggctaccacc acttttggag aaatctgttg gttagtgttg gcgtcgatat gtggcgcacc
      901 gccctcactg cgattgtagc tattagttgc cgcattgtag tcgtagtgag cgttataaac
      961 tggcccggac aaagttaaat tgatactgcg agcgttgggc gtcttgctcg gtgcttcggg
     1021 cttccgggga aagccggtga agttgctggt ggtatagccc ttgctggttt ccagctgatt
     1081 aagagcggca attgacgtat acatgttgtg cggtgctgcc cgactgctaa tgcgatggta
     1141 agcgccggca ttggcaaatt catcgaggtc tttcacgccc caggctttta tgtcagctag
     1201 accgtcctgg ctacccccaa cgtgggcata agcggcgtca aagcccatag cccactggat
     1261 atagtagggg cgagcactgc gcaccgggcc gatgttgcta ggcgcagtat cctgaaacag
     1321 tgccaggaag cgggtcaccc caccttcggc aatggcctca aagaccacgc cggcttgccc
     1381 taacccggat tgcggtcggg caaaagtagt attttcgatc atgacgccgg tgactggccg
     1441 ctgattgaca ctggggtcga cgggtaaacc ggttaaagtt gatggcacag tggtcggctt
     1501 aggcaccggc ttgggcttgg taaccttgat ggctgaagcg acgggcttag gtcccgtgtg
     1561 ggtcagtacc aagccaataa tgctgccgca aaccacgacg acaatggccg aggccagcca
     1621 ctccttctgc cccggtggcc actgtagcct gaatttgtcg cgccagctgg tggttttttt
     1681 cggtgagaga tgctcatctg tcatagccgg cagctcggct agcggcgcag gcgtagtaat
     1741 gtcatcgaca gccgctacct cctccggagt gtggaatgat ttgtttggga gtggcggttt
     1801 tgatgtttgg gccgtgtctg atggtgacgg ccgaacaggc cgttttatcg gccggaaatc
     1861 ggaatccatt gtcttattat aacaacgctt atgctaagag gctagtccgc tagaggctaa
     1921 aacagcgaat aaatggcggc aatacccgaa actgccatga gcaaggtgat gagccagccc
     1981 aaggcagtta cccaaggctt gttagtccag tgacccataa tcttatgatt actgctgatc
     2041 aggatgatga ggagcaggat gatcggtgct accaggccat tcccgaccgc agcgtagatc
     2101 agtgctttta tagggtcgag tccgatgaaa ttgatggcta agccaacgaa catcgagatg
     2161 ataataatgc cgtaaaaggc ataggcttgt ttgaggttgc gatacaaccc ttcccgccag
     2221 cggaaactct cagacaaggc ataactactt gagcccgcca gcacgggaat tgccaagagg
     2281 ccggtgccaa tgataccggc ggcaaacaga aagtaagtgg cattgccggc aaagggtcgc
     2341 agggcttcgg cagcttgggc agaagtctgg atgttggtga tgccgtgggt gaacaagatg
     2401 ctgccgcagg cagcaatgat gaaaaacata accagttcag ataagaacat gccggaccaa
     2461 acgtctatgc gcatactctt catgtcagcg ggatcggctg ccgtgcgacg ttcttctatc
     2521 gtagtttttc cttgcaaaat ttgttcttca acttcttggg aggtttgcca aaagaagaga
     2581 tagggtgaaa tagtggtgcc aagtatagcg caaaccagca gcagctggtc ttttgaaaag
     2641 gtgacacttg gtaaaaaggc gtgcttgaag acagtatgcc agttgaggtg cgccagtatg
     2701 gccgacaaaa cgtaactaaa tagcaccatt gccagccact tcagatatct cgcatagcgt
     2761 acgtacggcg tatatatttg tataagtaag ctcaagagag ttataaaaat aaccagcatg
     2821 ctaaagtcta agcgcggatt gagtagctgc acggctttgg ccatggcccc gatatcggcc
     2881 ccgatattca aagcgttggc cgcaaacaat agcgtggtac atacatagag tattggttta
     2941 ggaaagtagg ctcggatgtt gccggcaagc cctctaccag ttacaagtcc gatacgggcg
     3001 cacatttcct ggacaacagc cgaaagcggg aatgtccaca aggatagcca taggagttga
     3061 aagccgtatt gggcgccagt ttgtgagtag gtggcgatac cagagggatc gtcatccgag
     3121 gcaccagtag tcaggcccgg gccgagcatg tgccaatagt cttcagtttt ccggagaaga
     3181 tcgttggagg gcaaggcggt ggctattttt tgggtggcca ctacggtgag gtccaaaatc
     3241 tccgccggcg cttcggcact tcgttgcaga atgtcttttt gtttcgtcat tttcgtgtaa
     3301 cgttagtatc tagtattgta gcacgtgacc atttactcgg cgcagcgact ggtattgacc
     3361 gtctggtttg ttgacatggc ttgtctgatt tcactactga ccttgctcgt tgtcagagcg
     3421 tagccgacgt gctggtaggt ggtggattgg gcgaaaataa caccgatgac cgtgccg
//
LOCUS       DATNGP010000167         3309 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_3571951, whole genome shotgun sequence.
ACCESSION   DATNGP010000167 DATNGP010000000
VERSION     DATNGP010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3309
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3571951"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..444
                     /locus_tag="VN778_04275"
     CDS             <1..444
                     /locus_tag="VN778_04275"
                     /inference="COORDINATES: protein motif:HMM:NF039821.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5663 domain-containing protein"
                     /protein_id="HXR50214.1"
                     /translation="EQYFEAKDDAGAFKWLETNFPNYKDIVQEEFDKLKAEVKQSAPQ
                     ILAASQSAAAQAQPEPVAPPPAGPPQPLADPVAFQQTPPAPQPTPQPPQPANPYPPQP
                     APGAMPGSAPVPPPPPPMPPTEPLPEQPPYPGFPPQQPPAGPGPA"
     gene            458..1033
                     /locus_tag="VN778_04280"
     CDS             458..1033
                     /locus_tag="VN778_04280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50215.1"
                     /translation="MVIPSAGAPTINYDELHRKLQSASSQFQLFHKVANLPFEYKLEM
                     ALLFLGLISLYVADPEEGVVKLAAVSDTEHYRISVQNYKFDPASFKVPLTDHDNSIVK
                     AIKQAKAQSTTDWATLSRANVHPKYSRDNQASSGIAHNTVYPLDVPGGGAMLFAYYQY
                     QEDMGQPQEEFMGKYSALVSAVLKRRLHELA"
     gene            1074..1358
                     /locus_tag="VN778_04285"
     CDS             1074..1358
                     /locus_tag="VN778_04285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50216.1"
                     /translation="MDSVKGGLMLMADFEGYRKQRYYDPELVEFNARISLLISGEIEP
                     LGDKLMRDAQDGDPDGGEFARLVPPDIPPHLSSIALRPPEGEPLLDLQAA"
     gene            complement(1355..2275)
                     /locus_tag="VN778_04290"
     CDS             complement(1355..2275)
                     /locus_tag="VN778_04290"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF012760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HXR50217.1"
                     /translation="MHKISATVVFFGSGPVAAKALALLAEDFEIEAVITKPQPAHHKE
                     DFPVLATSQKLGLKTFTPRNKVELSELFRQKPVRSRLGVVIDYGIIIAQDVIDYFPLG
                     IVNSHFSLLPQWRGADPISFSILSGQKKTGVSLMLIVEALDEGPLLAQSEWLIEPDVT
                     TPKLSEELIELSHELLKAMLPLYVAGKTEPAEQAAVTIADSKEPTYSRKLTKQDGMLD
                     FSKPAEHLEREVRAFAGWPRSRTAIGDVTVIVTNAHVSEGQGEPGKLWLQDKQLGFYT
                     ARGVLVIDRLIPAGKKEMSAAAFLAGYKLR"
     gene            complement(2238..2810)
                     /gene="def"
                     /locus_tag="VN778_04295"
     CDS             complement(2238..2810)
                     /gene="def"
                     /locus_tag="VN778_04295"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HXR50218.1"
                     /translation="MTKDDIIDLPNPHLRHKSKRVGLITDEIKQIVQDMQDATISWDQ
                     SRDHEVGVALAAVQIDRLYKIVVVRNNYDNKEDQTFTVFINPQITKREGEIVEDFEGC
                     LSVPNVYGKVPRHKKVKVKARDLQGKEFRVTAEDFLARIFQHEVDHCEGIVFIDHIKD
                     KPEAFFRLNDEGKLEPLDYAQDIRDSRLLW"
     gene            2898..>3309
                     /locus_tag="VN778_04300"
     CDS             2898..>3309
                     /locus_tag="VN778_04300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50219.1"
                     /translation="MSETVPFPNREINDGQKEIFAQAIERLEPFITLDTPTPEQLLAM
                     DERRDQQIEVHKIGHFVMDGTRFTLEISEVIDETDEGLATLHTIWSTKVQSAPVKVAD
                     QQFVAEKLYFVVKFEADDSWMAEYKGHTTVQGPQD"
BASE COUNT          796 a          903 c          836 g          774 t
ORIGIN      
        1 gagcagtatt ttgaagctaa ggatgacgcc ggtgctttca aatggctgga gactaacttc
       61 ccaaattata aagatatcgt ccaagaagag ttcgacaagc tgaaagccga ggtcaagcag
      121 tcggcgccgc aaattttggc agctagccaa agtgctgctg cccaggcgca gccagagccg
      181 gtggccccac cacccgccgg gccgccacaa ccgctggccg atccagtcgc ttttcagcaa
      241 accccacctg cgccgcaacc tacaccgcag ccgcctcagc ccgctaaccc ctatccgccg
      301 caaccagctc ccggagccat gccaggcagt gcaccagtac caccaccgcc acctccgatg
      361 ccgccaactg aacccttgcc tgagcagcct ccataccctg gttttcctcc ccagcaacca
      421 cccgctggtc ccggaccagc ctgatatact ggatagtatg gttattccgt cggctggtgc
      481 accgactatt aactacgatg aactccaccg taagttacaa tcggcttcca gccaattcca
      541 gctattccac aaggttgcga atctgccatt tgagtataag ctagaaatgg cccttttatt
      601 tttggggctc atcagtctct atgttgccga ccctgaagag ggtgtcgtga agctagccgc
      661 tgtgtccgac accgaacatt accggatttc ggtgcagaat tacaaatttg accccgctag
      721 ttttaaagtg ccgctcacgg atcatgacaa tagcatcgtc aaggctatta agcaggccaa
      781 agcccaatcc acaacagatt gggcgacgct gagccgggcg aacgtccatc ccaaatattc
      841 ccgcgacaac caagctagca gtggcatcgc gcataacact gtttatcccc tcgatgttcc
      901 aggcggcggc gccatgcttt tcgcatacta ccagtaccag gaagacatgg gtcaacccca
      961 ggaggaattt atgggaaaat acagtgcgct ggtctcggct gtactcaagc ggcgcctcca
     1021 cgaactagct taagctaaaa atcttacagc caagccttcc gataagcatt agcatggata
     1081 gtgtaaaggg agggctgatg cttatggctg attttgaggg gtatcgtaaa cagcggtatt
     1141 atgatccaga gctagtcgaa ttcaatgccc ggattagttt attgattagt ggagaaatcg
     1201 aacccttagg tgataaattg atgcgggacg cgcaagacgg tgacccggat ggtggcgaat
     1261 ttgctcgatt agtgccgcca gacataccgc cgcatctcag cagcatcgcg cttcggccgc
     1321 ctgaggggga gccgcttttg gatctgcaag ctgcctaacg aagtttgtat ccggcaagaa
     1381 atgccgctgc cgacatttcc tttttgccgg ccgggatgag gcggtcaatc accaaaacac
     1441 ctcgagcggt gtaaaagccg agctgtttat cctggagcca tagtttgccg ggttcaccct
     1501 ggccctcact gacgtgagcg ttagtgacaa taacagtaac atcgccaatt gctgtgcggc
     1561 tgcgcggcca gccagcgaag gcgcggacct cgcgttcgag atgctcggct ggcttgctga
     1621 aatcaagcat accgtcttgc ttggtaagtt tacgggaata cgtcggttcc ttggagtcgg
     1681 ctatagtgac ggcggcttgt tcggctggct ctgttttacc ggctacataa agagggagca
     1741 tagcttttaa taattcatgg cttaactcta tcagttcctc gctgagtttg ggcgtggtga
     1801 catccggctc aatcagccat tcgctttgcg cgagcagggg accttcatcg agcgcttcaa
     1861 cgatcagcat caggctgaca ccagtctttt tttgaccgct taggatgctg aaactgatcg
     1921 gatcggcgcc acgccactgg ggcagcaagc tgaagtgact gttgacgata ccgagtggaa
     1981 aataatcaat aacatcctgg gcaataatga tgccgtagtc gatcaccaca cccagccggc
     2041 tgcggacagg tttttgcctg aacagctctg atagctcgac tttgttacga ggcgtaaaag
     2101 ttttcagacc aagcttctgg ctggtagcga gtacagggaa gtcttccttg tgatgtgccg
     2161 gttgcggctt ggttatgacg gcctcaattt caaaatcttc agccagcagc gccaaagcct
     2221 tggccgccac cggcccgcta ccaaagaaga cgactgtcgc ggatatcttg tgcatagtcg
     2281 agtggctcca atttgccttc gtcattcagc cggaagaaag cctccggctt gtctttgata
     2341 tgatcgatga aaacgatgcc ttcgcagtga tcaacctcgt gttgaaagat gcgcgccaga
     2401 aaatcttcgg cggtgacccg gaactcttta ccctgtagat cgcgcgcctt gaccttgact
     2461 tttttatgcc gcggcacctt gccataaaca ttaggtacgc ttaagcagcc ttcaaaatct
     2521 tcaacgattt caccctcgcg cttggtgatc tgcggattga tgaaaacggt aaacgtctgg
     2581 tcttctttat tgtcgtagtt gttgcgaacg accacgattt tgtagagccg gtcgatctgc
     2641 accgccgcga gcgccacgcc cacctcgtga tcccggctct gatcccagct aatcgtcgca
     2701 tcctgcatat cctgcacgat ttgcttaatc tcgtcggtga tcagcccgac gcgcttggac
     2761 ttgtgcctga ggtgcggatt gggcaggtca atgatgtcat ctttggtcat attccgaata
     2821 gtatacgtca gaatcgcatc tgttaaaagc cagcttttta ttttgacacc ataaaaattc
     2881 aatattacaa tgatcatatg tctgaaacgg taccgtttcc aaatcgggag atcaacgacg
     2941 gacaaaaaga aattttcgcg caggctattg aacgtctaga gccctttatc acgcttgaca
     3001 ccccgacacc ggaacagttg ctggcgatgg acgagcggcg cgaccaacaa attgaagtgc
     3061 acaaaatcgg tcactttgtc atggacggca cccgtttcac gcttgaaata agtgaggtca
     3121 ttgatgaaac cgatgaaggc ttggccaccc tgcataccat atggtcgact aaggttcaat
     3181 cagcgcccgt aaaagtagcc gatcagcagt ttgtcgccga gaagctttac ttcgtcgtca
     3241 agttcgaagc cgatgatagc tggatggccg aatacaaagg tcacaccacc gtgcaaggac
     3301 cgcaggatc
//
LOCUS       DATNGP010000168         7220 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_1315202, whole genome shotgun sequence.
ACCESSION   DATNGP010000168 DATNGP010000000
VERSION     DATNGP010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 7220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7220
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1315202"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..386
                     /gene="rpsL"
                     /locus_tag="VN778_04305"
     CDS             <1..386
                     /gene="rpsL"
                     /locus_tag="VN778_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HXR50220.1"
                     /translation="PRSKANPKSKSPALQRVTNNLKTRNYEKAAPFKRGVCVKVTTKT
                     PKKPNSALRKVARVRLSNGYEVWAYIGGEGHNLQEHAVVLVRGGRVKDLPGVRYHVVR
                     GNLDLQGVNDRKQGRSKYGTKKAGK"
     gene            386..865
                     /gene="rpsG"
                     /locus_tag="VN778_04310"
     CDS             386..865
                     /gene="rpsG"
                     /locus_tag="VN778_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HXR50221.1"
                     /translation="MPRKKTKSLVRDIHPDHVYDSVAVQRVINRVMLNGKKQLAERLV
                     YGGMQKAANKLKVDNPLDVFEQAMKNVQPHLETRSRRVGGANYQIPFEVKGQRQRHLT
                     TMWFVQAARSRKGMSMADRIAAELMDAYNNQGAAVKKREDTHKMAEANRAFAHFARS"
     gene            911..1180
                     /locus_tag="VN778_04315"
     CDS             911..1180
                     /locus_tag="VN778_04315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50222.1"
                     /translation="MCDQAFADCADSLNAMGERYLHDDFTEPGRAEEVLNFVAFSQES
                     TVKMLKHVLKGSFGYDCQLTDEQIIARVRETQKFRQAAQKDIGEI"
     gene            1181..3268
                     /gene="fusA"
                     /locus_tag="VN778_04320"
     CDS             1181..3268
                     /gene="fusA"
                     /locus_tag="VN778_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017754866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HXR50223.1"
                     /translation="MAAKKTAIQDIRNLGIIAHIDAGKTTTTEGILYRTGLKHKIGAV
                     HEGETTTDWMAQERERGITIVAASVTCYWKAHQINIIDTPGHIDFTVEVERSLRVLDG
                     ACVVFDGKMGVESQSETVWRQADKYGVPRICFINKINQTGGDFYKSLDSIHNRLSKRA
                     FPVHLPIGFEQSINGVVDLIEMKAYTYKDFTDHELTEGEIPADMLDQAKKYRSLLVEN
                     AVAEDEAMLEKYFEVGEEGLSTDEIKQAIRTAVLTGKFFAVTGGDGRGVIVEKVLDMV
                     NDYLPSPLDKGSVWGTHPKTGDEIERKPSESEPFASLAFKITTDPFVGKLAYFRVYSG
                     KVLAGSYVLNSSTGEKERVGRIVRLQADKRDEVQEVGAGDIAAIVGLKGTTTGNTLSD
                     PNNPIVLESITFPEPPVSIAIEPKTKADQEKMSLALQRLAEEDPTFRIRVDDETGQTI
                     ISGMGELHLEILVDRMKREFNVEANIGQPQVAYRETIRKDGVEVQGKFVRQSGGRGQY
                     GDVWLRLSQNEAGKGFEFINSIKGGIVPSEYIPAVRKGIEEAMSNGVIAGYPVVDIKA
                     ELYDGSYHDVDSSEMAFSIAGSMALQDGVKKATPVLLEPVMKVVVVTPEEFMGDVIGD
                     LNSKRGRIESMEDLTPGIKEITGFVPLGEMFGYTTNLRSMTQGRASSSMELDHFADVP
                     NNVAEAIIAKNAK"
     gene            3713..4897
                     /gene="tuf"
                     /locus_tag="VN778_04325"
     CDS             3713..4897
                     /gene="tuf"
                     /locus_tag="VN778_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003806883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HXR50224.1"
                     /translation="MADQFDRSKPHVNVGTMGHVDHGKTTLTAAITAVLAKKLPSAIN
                     KPIAYDQIDNAPEEKARGITIATSHQEYESEKRHYAHVDMPGHADYVKNMITGAAQVD
                     GAILVVSAADGPMPQTREHVLLAKQVGVPYILVFLNKMDLADPELVDLVEEEVRELLE
                     KNDFDRNAPIIRGSATKALEGDAANEDAIMELVKAMDENVPEPKRELDKPFLMPIEDV
                     FSIKGRGTVATGRVEQGVVKVNEEVEIVGIRDTRKTVVTGVEMFKKNLDQGQAGDNVG
                     ILLRGIERDDIERGQVLAKPGSITPHTEFDSEVYILTKEEGGRHTPFFKGYKPQFYFR
                     TTDVTGEVELPADKEMVMPGDTVTFKVKLLAPIAMEQGLRFAIREGGRTVGAGVVTKV
                     VK"
     gene            5046..5357
                     /locus_tag="VN778_04330"
     CDS             5046..5357
                     /locus_tag="VN778_04330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50225.1"
                     /translation="MEQNVQEVQKVQETSSTNPLDDILNEWLCPNCPDREVLREEIQR
                     GITSQQALMKHVGRFAMRYLEASVCEPGQTQAACGNQAVRGALSFIFEAQAILRDSRA
                     S"
     gene            5487..5816
                     /gene="rpsJ"
                     /locus_tag="VN778_04335"
     CDS             5487..5816
                     /gene="rpsJ"
                     /locus_tag="VN778_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015642179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HXR50226.1"
                     /translation="MADAKPVETKQRIRIRLKAYDHKVIDQAAKQIVDTALRTGASLA
                     GPIPLPTRKSTFTVIKSPHVYKKGREQFEMRVHKRLIDVFEPTPKTIDSLMNLSLPAG
                     CDVEIKM"
     gene            5834..6076
                     /locus_tag="VN778_04340"
     CDS             5834..6076
                     /locus_tag="VN778_04340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50227.1"
                     /translation="MSETEHSFAKRDGHVPCELRQYPASEVMKDGCVETGPEVLRCLR
                     LANGNIGLVFPRIETDHPVELAALEEAFENSNQTEL"
     gene            6178..6450
                     /locus_tag="VN778_04345"
     CDS             6178..6450
                     /locus_tag="VN778_04345"
                     /inference="COORDINATES: protein motif:HMM:NF024143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HXR50228.1"
                     /translation="MFARSHSIKDHPGQLVGTAGLAAALGAIGAVLFTPKRGSEVRQD
                     IRERGKRMKDKMSHMKDTKERAKVVARRGKQTSGDMADEIRRKGEP"
     gene            6636..>7220
                     /gene="rplC"
                     /locus_tag="VN778_04350"
     CDS             6636..>7220
                     /gene="rplC"
                     /locus_tag="VN778_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015642173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HXR50229.1"
                     /translation="MKALITRKIGMTSTINEDGTVQAVTLLLVGDHTVLAHKTEEKDG
                     YTAVQVGAEQAKKLSKSVAGHVKNAKVMPKLIREFRFDEISEDLSVGSKLSADVFSVG
                     DDVQVTGITKGKGWAGTIRRHNFHRGRKTHGGRSYRRPGSIGSMYPQHIFKGTKMAGQ
                     LGHDQVTVKGLKVALIDTEQNVIGVTGAVPGPRKG"
BASE COUNT         1942 a         1755 c         1927 g         1596 t
ORIGIN      
        1 agccacgtag caaggcgaat ccgaagagta aatcgccagc gctgcagcgt gtcacgaaca
       61 acctcaagac taggaactac gaaaaagccg cgccgttcaa gcgcggggtc tgtgttaagg
      121 taaccaccaa aacaccgaaa aagccgaact cggccttgcg taaggtcgct cgcgtgcgcc
      181 tttcgaatgg ctacgaagtc tgggcctaca tcggcggcga aggccacaac ctccaagaac
      241 atgccgttgt tctcgtccgt ggcggccgtg tcaaagatct tcccggtgtc cgctaccacg
      301 tagtccgtgg caacctcgat ctccaaggtg tcaacgaccg caaacaaggc cgctccaagt
      361 acggcaccaa gaaagcaggc aaataatgcc taggaagaag actaaatcac tcgttcggga
      421 tattcatccg gaccatgttt acgatagtgt cgccgtccaa cgcgtcatca accgtgtcat
      481 gctgaatggc aaaaagcagc tggctgagcg cctggtgtat ggcggtatgc aaaaagctgc
      541 caataagctg aaggtagaca atccactcga tgttttcgag caggccatga agaatgttca
      601 accccacctt gagactcgtt cgcgccgcgt cggcggcgcc aactaccaga ttccgtttga
      661 ggtcaaaggt cagcgccaac gccacctaac gactatgtgg tttgttcaag ctgctcgctc
      721 ccgtaaaggc atgagcatgg ccgatcgtat cgcggcggag ctgatggatg cctacaacaa
      781 ccaaggtgcg gccgtgaaga agcgtgaaga cacccataag atggccgaag ccaaccgcgc
      841 cttcgcccac ttcgctcgga gctaaataat gaccgaacga tgctcctcat gtaatacagg
      901 cttacagctg atgtgtgatc aagcttttgc ggactgcgca gacagcctca atgcaatggg
      961 tgagagatat ttgcacgacg atttcacgga accaggcagg gctgaggagg tactgaattt
     1021 tgtggctttt tctcaagaga gcaccgttaa gatgttaaag cacgtgttga agggttcatt
     1081 tggctacgac tgccaactaa ccgatgaaca gataattgca agggttagag aaactcaaaa
     1141 atttagacaa gcagcccaaa aggatatagg agaaatataa gtggcagcaa agaagacagc
     1201 aattcaagac attaggaacc tcggcattat cgcgcacatt gatgcgggta aaacgaccac
     1261 gaccgaaggc attctttatc gtactggttt gaagcataag attggtgccg tacacgaagg
     1321 tgaaacgacg accgactgga tggctcagga gcgggagcgt ggtattacca tcgtggcggc
     1381 cagcgtgacc tgctactgga aagctcatca gatcaatatt atcgatacgc caggtcacat
     1441 cgacttcact gtcgaggtgg aacgctcgct gcgtgtgctt gatggcgcct gcgtggtgtt
     1501 tgacggcaaa atgggtgtgg aatcgcagtc tgagactgtg tggcgccagg ctgacaaata
     1561 cggcgtgccg cgcatctgct ttattaataa gattaaccag acgggcggcg atttctacaa
     1621 gtcgctggat tctattcata atcgcctaag caaacgcgct ttcccggtac atttgccaat
     1681 cggctttgag cagtcaatca atggtgtggt cgatttgata gagatgaaag cttatacata
     1741 taaggacttt accgaccacg agttgacaga gggcgaaatc ccagctgaca tgctggatca
     1801 agctaagaag tatcgctcac tcctcgtcga aaatgctgta gcagaagatg aagccatgct
     1861 tgagaaatat ttcgaagtgg gcgaagaggg cctcagtact gatgaaatta agcaggccat
     1921 acgcactgcg gtgcttaccg gtaagttctt tgcggtgacc ggtggtgatg gccgcggcgt
     1981 gattgttgaa aaggtactgg acatggtcaa tgactatttg cccagcccac tcgataaagg
     2041 ttcagtctgg ggcacgcatc ccaaaaccgg ggacgaaatt gagcgcaaac caagcgaatc
     2101 tgagcctttt gcctcgctgg ctttcaaaat tacaaccgac ccgtttgttg gtaagctcgc
     2161 ttacttccgt gtatattccg gcaaagtttt ggctggaagc tacgtgctaa acagctcgac
     2221 tggtgaaaaa gagcgggtgg gccgtatcgt gcgtttgcaa gccgataagc gtgacgaagt
     2281 gcaagaagtg ggcgctggtg acatcgcggc tatcgtgggc ttgaaaggta cgacgaccgg
     2341 caatacgctg agcgatccca acaatccgat cgtgctcgaa agcattactt tcccagagcc
     2401 gccggtctct atcgctatcg agcctaagac aaaagccgat caagaaaaga tgagcttggc
     2461 cctgcagcgt ctcgctgaag aagatccaac ctttcgtatc cgggtcgacg atgagacagg
     2521 acaaaccatt atctccggta tgggggagct gcaccttgaa attctcgtcg atcgcatgaa
     2581 acgcgaattt aacgtcgaag ctaacatcgg ccagccgcag gttgcttacc gtgagacaat
     2641 ccgtaaagac ggtgttgaag ttcaaggcaa gttcgtccgc cagtccggcg gtcgcgggca
     2701 gtacggcgat gtctggctgc gtctcagcca aaatgaggcc ggcaaaggct ttgaattcat
     2761 caactccatc aaaggtggca tcgtgcccag cgaatacatt ccggccgttc gcaaaggtat
     2821 cgaagaagcc atgagtaacg gcgtcattgc cggctatccg gtcgtcgaca tcaaagccga
     2881 gctctacgat ggcagctatc acgacgttga ctcttctgaa atggcctttt ctattgccgg
     2941 ttcgatggct ttgcaggatg gcgtcaagaa agctacaccc gtactgctgg agcccgtcat
     3001 gaaagtcgtc gtcgtgactc ccgaagaatt catgggcgac gtcatcggtg acctcaactc
     3061 gaagcgtggt cgcattgaat ccatggaaga tctgacccct ggcattaaag aaattaccgg
     3121 tttcgtcccc ctaggggaga tgttcggtta caccaccaat ctccgatcca tgactcaagg
     3181 tcgtgccagc tccagcatgg agcttgacca cttcgctgat gtgccgaaca acgtcgccga
     3241 agccatcatt gccaagaacg ccaagtaaaa ctaaccacta agaagtaagg gatcgaagtg
     3301 cacaatcaag aagagttaaa aaggcgagcg gcaaaggtcg tgcgctggaa tgacagtctg
     3361 gagcgaggcc tggaaagcag agtaaaccat cgtaatacag caggtaaatc attccccaag
     3421 atagtgggcg gggagcttag ttaaggaaaa gctatctaaa ctcaagggag aaatgttagt
     3481 tttcccagac ggcagaactg ttgagaccac tgatttagca aaagaaatta cggaatcgca
     3541 tcaggggtcc gcagtgcccg tacaaccgca aatttccagg attgcctaac tctttacaaa
     3601 gaccgccgga ttctctatac tatctcgagt aactcgcgtg agttgttgct cacgtgtata
     3661 tagtaatcga gtaagaatgt taattaataa gctatttaaa ggagcataac atatggcaga
     3721 ccaatttgac cgcagcaagc cgcacgttaa cgtcggcacc atgggccacg tcgaccacgg
     3781 caaaacgacc ttgacggcag caattaccgc tgttctcgca aagaaactac caagcgcaat
     3841 caacaagccg attgcctatg atcagatcga taatgctcct gaggaaaagg ctcgtggtat
     3901 cactatcgct acgtctcacc aagagtacga gagcgaaaaa cgccactatg cccacgttga
     3961 catgcctggc cacgccgact atgtcaagaa catgatcacc ggtgccgctc aggttgacgg
     4021 cgctattctg gtcgtgtctg ccgcggacgg tcctatgcct cagactcgtg agcacgtcct
     4081 gttggccaag caggttggcg tgccgtacat cttggttttc ttgaacaaga tggacctggc
     4141 tgaccccgag ctcgtcgacc tcgttgaaga ggaagtgcgg gagctgttag agaagaatga
     4201 tttcgatcgc aatgcgccta tcattcgtgg ttctgccacc aaggccctcg aaggcgatgc
     4261 tgccaacgaa gacgctatca tggagctcgt caaagctatg gacgaaaacg ttcctgagcc
     4321 aaagcgcgaa ctagataagc cgttcttaat gcctatcgaa gatgtattct cgatcaaagg
     4381 tcgtggcacg gtggctaccg gccgggtaga gcagggtgtc gttaaggtga acgaagaagt
     4441 tgagatcgtc ggcatccggg acactcgcaa gaccgttgtg accggtgttg agatgttcaa
     4501 aaagaacctt gatcaaggcc aggcaggcga taatgtcggt attctacttc gtggtattga
     4561 gcgcgatgac atcgagcgcg gccaagtttt ggccaagccc ggcagcatta cgccacacac
     4621 cgaatttgac tctgaagtct acatcctgac caaagaagag ggtggtcgtc acaccccgtt
     4681 ctttaagggc tacaagccgc agttttactt ccgaaccacc gatgtgaccg gcgaagtcga
     4741 attgccagct gacaaagaaa tggttatgcc cggcgacacc gtaaccttta aggttaagct
     4801 gttggcccca atcgctatgg agcaaggctt gcgctttgct attcgtgaag gcggtcgcac
     4861 cgttggtgcc ggagtcgtta ccaaggttgt caaataagca ttcagcttaa gctttgacaa
     4921 aaagagcctc gcaaggggct cttttcaaga gctttactta cagacaaatt tcttctgaaa
     4981 ctgagatact atcttgatag tctttcgacc aggctattta ttccttgact aagaaaagag
     5041 gcgacatgga gcaaaacgtt caagaagtgc aaaaagtgca ggaaacatca tccaccaacc
     5101 cgcttgatga catactaaat gagtggcttt gtccgaattg tccagatcgc gaagtgcttc
     5161 gtgaagaaat ccaacgaggg attactagtc agcaggcact gatgaagcat gtaggtaggt
     5221 tcgcaatgcg ttatcttgag gcttcggttt gtgagccagg tcagactcag gcagcttgtg
     5281 gtaatcaggc ggtaagaggc gcgctcagct tcatatttga agctcaagcc attctgcggg
     5341 atagcagggc tagctaaagt gtatagaatc tgttagagta gtgtcatact atgtaagtta
     5401 aattgctgac taaaagttac ctttgtatga agaatgtgta atgttacagc agttcccgct
     5461 tgcgtgcggg gaaaggaaca ccacctatgg cagacgccaa accagtagaa accaaacaac
     5521 gaatccgtat tcgcctgaag gcctatgatc ataaagttat tgatcaggct gccaagcaga
     5581 ttgtcgatac ggcactgcgc accggcgctt cattggccgg gccgatcccc ttgccgacgc
     5641 gcaaaagcac ttttacggtt atcaagagcc cgcacgttta taagaaaggc cgcgaacaat
     5701 tcgaaatgcg cgtccataag cgcctcattg acgttttcga accaactcct aagaccatcg
     5761 attcgctgat gaatctgtcc ctacctgccg gctgtgacgt cgaaattaag atgtaagatt
     5821 acaggattcc ataatgtcag agacagagca ttcatttgca aaacgagatg gtcacgtgcc
     5881 gtgtgagcta cggcaatatc cggcctcaga agtcatgaaa gacggctgcg tagaaacggg
     5941 gcccgaggtg ctacgctgcc tgcgattagc aaacggaaac atcggtctag tttttccacg
     6001 catcgaaact gatcatccgg tggagctcgc cgctctggaa gaggctttcg aaaattctaa
     6061 tcaaactgag ctataaaagc cgaacactag ttggccggtt gtgagcaatc ttatagatgg
     6121 tcacccagcg aacctcttat aattatcttt gtaatcgtaa tcgaaggagg tgccgcaatg
     6181 tttgctcgat cacattcgat taaagatcat ccgggacaat tggttggaac tgctggtctc
     6241 gcagctgctt tgggggccat tggcgcggtc ctgtttaccc caaagcgtgg ttcggaggta
     6301 cgccaagata tccgtgagcg tggtaaaagg atgaaggata agatgtcgca tatgaaggat
     6361 accaaggaaa gagctaaagt tgttgcgcgg cgtggcaaac aaactagcgg cgatatggcg
     6421 gatgagattc ggcgcaaggg cgagccataa aaacgaagct tcaagtttgt agaatttgca
     6481 acgcattgac aggggtaagc taagcgtgtt actctagtac ggtatctcgg tgcagactgg
     6541 tacttggtta gcgccagcag gtctactcat aacaggggta gaaaccgctc gcaaccaggc
     6601 gtttttatgt ctaaccttgg cacagaaagt aatcaatgaa agcactcatc actcgcaaaa
     6661 tcggcatgac cagtaccatc aatgaagacg gtacggtaca ggctgttacc ttgcttttgg
     6721 tgggcgatca tactgttttg gctcacaaga ctgaagagaa ggatggttac acggccgtcc
     6781 aagtcggtgc cgagcaggca aagaagctat ctaagagtgt cgcgggtcac gttaagaacg
     6841 ctaaagtgat gccgaaactc attcgcgaat tccgcttcga tgagatttcg gaagatctga
     6901 gcgtcggcag caaacttagc gccgacgttt ttagtgtggg agatgacgtc caagtgactg
     6961 gcatcacaaa aggtaaaggt tgggccggca ccattcgtcg ccacaacttc catcgcggcc
     7021 gtaagacaca tggtggtcgg agctatcgcc gtccgggctc catcggctcc atgtatccgc
     7081 agcacatttt caaaggaaca aagatggctg gccagttggg tcacgatcaa gtcaccgtta
     7141 agggccttaa ggttgccttg atcgataccg agcaaaatgt catcggcgtt accggcgctg
     7201 tgcccggtcc tcgcaagggc
//
LOCUS       DATNGP010000169         2361 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_3269650, whole genome shotgun sequence.
ACCESSION   DATNGP010000169 DATNGP010000000
VERSION     DATNGP010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2361
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3269650"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..665)
                     /locus_tag="VN778_04355"
     CDS             complement(<1..665)
                     /locus_tag="VN778_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008579150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde 3-phosphate dehydrogenase
                     NAD-binding domain-containing protein"
                     /protein_id="HXR50230.1"
                     /translation="MKTRVAINGFGRIGRNAFKVAFERSDIEIVAINDLTIPATLAHL
                     LKHDSNYGTYDKEVGFDDQGIVVDGQHIKIFAERDPAALPWGEHKVDVVIESTGFFVD
                     PAKARAHVDAGAKKVVISAPAKGEGATTIVLGVNEDKLEEASDIISNASCTTNCITPV
                     AAVIENHFGIEKAMMTTVHSYTASQALQDGPGAKDKREMRNAAENIVPTTTGASIAAG
                     QALP"
     gene            complement(734..1171)
                     /locus_tag="VN778_04360"
     CDS             complement(734..1171)
                     /locus_tag="VN778_04360"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HXR50231.1"
                     /translation="MRQNALRSKIVAKAIIQNEQGKVLLLRRSQTDTRRPGEWDFPGG
                     GIESGEEIIAGIIREIHEEAGLSFTTEALQLVYAATEPYAPSQESVTRLLFYGQTSMT
                     GIQLSFEHDHSKWESIEKALIDFPHPFYGRGLAYARDNGLLKS"
     gene            complement(1149..1688)
                     /locus_tag="VN778_04365"
     CDS             complement(1149..1688)
                     /locus_tag="VN778_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009766749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HXR50232.1"
                     /translation="MADFNQVLEPGDYQNGTVNAVIDIPMGSTLKIEWDRERACFVLD
                     RVEPSIYPKPVNYGFIPQTLDEDGDELDVLVFCNQPIPTGTWLETKIIGIFNFEDDGE
                     MDYKIVCVPADDRDTGDSINSLADLGERWQRKIEEHFAHYKDLKKPGTTKVLGWSDTE
                     AAKQVIKESIDRYEAKRSA"
     gene            1808..>2361
                     /locus_tag="VN778_04370"
     CDS             1808..>2361
                     /locus_tag="VN778_04370"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HXR50233.1"
                     /translation="MKIIGHRGARGLAPENTIASLQKGLEHHADMLEFDLRVTKDHVV
                     ILHHDPYLTDPNGKKIIIKDTTYAELLEHKADIPTFEEVLDKVGHPVRLYVEVKPEAE
                     TKPIIKIFKARLAKGWKPEYFYLASFSQKILLELDKDLPEVQKVVIENWSGVRAHWRA
                     RQLHTKTVCMNERWLWSGFIKSVQ"
BASE COUNT          569 a          631 c          545 g          616 t
ORIGIN      
        1 ggcaaagcct gaccggcggc gatcgaagca ccagtcgtgg taggtacaat attctccgcc
       61 gcattgcgca tctcacgctt atctttcgcg ccaggcccat cttgcaatgc ctgtgaggca
      121 gtatagctat gcaccgtggt catcatcgcc ttttcgatgc cgaaatggtt ctcaataacg
      181 gcggcgaccg gtgtaataca gttcgtggtg catgaagcgt tgctgataat gtcgctagct
      241 tcttctagct tgtcttcgtt gacaccaagc acgatggtag tggcgccttc gcctttagct
      301 ggagcactga tcacgacttt tttggcaccg gcatcaacgt gagctcgggc tttagccgga
      361 tcgacgaaaa agcctgtcga ctcgatgacg acgtcgacct tatgctctcc ccacggcagc
      421 gccgccgggt cacgctccgc aaagatcttg atatgctgac cgtcgacaac aataccctgg
      481 tcatcgaagc cgacctcctt gtcatacgta ccgtaattac tgtcatgctt taagagatgc
      541 gctagcgttg ctggaattgt caaatcgttg attgccacga tctcgatgtc actgcgttca
      601 aatgctacct taaaagcgtt gcgtccgatg cgaccaaagc cgttgattgc caccctagtt
      661 ttcatatttg ttccttatgt atgttttacg tttaaagctt atgactatat tgtaaaagtt
      721 cgctccttat cggtcaactt ttcagtaaac cattgtctcg ggcataagcc agcccccggc
      781 cgtaaaacgg gtgcgggaaa tcaatcagcg ctttctcgat actctcccat ttactgtgat
      841 cgtgctcaaa gctgagctgg atgcctgtca tgctcgtctg tccataaaaa agcaagcgcg
      901 ttacactctc ctggctgggc gcataaggct cggtggcggc atataccagc tgtagcgcct
      961 ctgtcgtaaa agacaaacca gcctcctcat gaatctctcg gataataccg gcgatgatct
     1021 cctcgccgga ctctatgccg ccaccgggaa aatcccactc gcccggccgc cgggtgtcag
     1081 tctgactacg cctgagcagc agcacttttc cctgctcgtt ttgaataatc gctttggcaa
     1141 cgattttgct acgcagagcg ttttgcctca tagcgatcaa ttgactcttt gataacttgt
     1201 ttagcggctt cggtatcgct ccagccgaga actttggtcg tgcccggctt cttcaggtct
     1261 ttataatggg caaaatgctc ctcgattttt cgctgccagc gctcgcccag atcggccaag
     1321 ctattgatgc tgtcgccagt atcacggtcg tcggccggca cgcagacaat tttgtagtcc
     1381 atctcgccgt catcttcaaa gttaaaaatg ccaatgatct tcgtttcgag ccaagtgccg
     1441 gtaggaatcg gctgattgca aaaaacgagt acgtcaagct catcaccatc ttcgtccaag
     1501 gtttgcggaa taaacccata gttcactggc ttcggataaa tgctcggctc tacccggtct
     1561 agcacgaaac aagcccgttc ccggtcccac tcgatcttaa gcgtcgagcc catcggaatg
     1621 tcgatcaccg cattcacggt gccattttgg taatcgcccg gctctagtac ttgattgaaa
     1681 tcagccatat gtttctcctc ccagcacttc cgttttggat cctacatttt ctgtacaaaa
     1741 tctgtacaga aaatgtacag agctttttta ctcggttatt aatctgacaa tattgtacac
     1801 tatgagcatg aaaatcattg gccatcgtgg tgcccgtggc ctagcgcctg aaaataccat
     1861 tgcgagcttg cagaagggct tggaacatca cgcggacatg ctcgaatttg acctccgcgt
     1921 caccaaagac cacgtcgtta tcctacatca tgacccctat ttaacagatc caaacggtaa
     1981 aaaaatcatc ataaaggata cgacttatgc cgaactcttg gagcataaag ccgacatccc
     2041 gacttttgaa gaggttttag ataaggtcgg ccacccggtc cggctatatg tcgaagtgaa
     2101 gcccgaagct gaaaccaagc cgataattaa aatttttaag gcacgcctag ccaagggctg
     2161 gaaaccagaa tatttttacc tggcatcatt tagtcaaaaa atcctcttgg aattggacaa
     2221 agacttgccg gaagttcaaa aggttgtgat agaaaactgg tcaggcgtca gagcgcactg
     2281 gcgggcccgt caacttcata cgaaaacggt ctgcatgaat gaacgctggc tgtggagtgg
     2341 ttttataaaa tccgtccaga g
//
LOCUS       DATNGP010000170         3393 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_2656432, whole genome shotgun sequence.
ACCESSION   DATNGP010000170 DATNGP010000000
VERSION     DATNGP010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3393
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2656432"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..510)
                     /locus_tag="VN778_04375"
     CDS             complement(<1..510)
                     /locus_tag="VN778_04375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50234.1"
                     /translation="MDKLNREKLKKLSSMETEPSVTIYLPLHTTASPPHISENQIRFK
                     NLVHRAKQQLSAHGTGQEVAKALEARLAELHDDLAFWEAGKPGLLICAAPEVMEMFYL
                     PIDTEEYVAVDNHFHIAPVLGLLHDARDFYVLTLAQRNPKMYRGTLYGLTPLRLNLPT
                     DAKAAFNIDE"
     gene            complement(628..999)
                     /locus_tag="VN778_04380"
     CDS             complement(628..999)
                     /locus_tag="VN778_04380"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXR50235.1"
                     /translation="MGTKVLLVEDEKLLRESYQIILEAESFDVDTAVNGLKALELCHA
                     NTYDLILLDIMMPELDGIGFLKKFIAKYKTLPTTIILSNLGHGDEINQALQLGAKKYI
                     QKSQLDPSGLVALLHEEVAAA"
     gene            complement(974..2188)
                     /locus_tag="VN778_04385"
     CDS             complement(974..2188)
                     /locus_tag="VN778_04385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HXR50236.1"
                     /translation="MKTVKQIKLAASNQNPAMQAKVEPADLYRLLVESIQDYAIFLLD
                     TNGMVMSWNAGAQKLKGYKANEIIGKHFSVFFLPKDRKDDKPRRELEAARQQGSTEEE
                     GWRLKKDGDRFWANVILTALYDNSGHLQGYAKVTRDLSERKQAEDNLKQANRELQKQQ
                     KKLEQLGRAREAFFSIVSHELRNPATVVKQYLGIMLAGYGGPLNPTQIDYLQKANNSN
                     DRQIVIANDLIKVAQVDAGKIVLHKKPTDVTELIELILSEHADMFHGRSQHGSFEAPP
                     APIIAEVDPARLRMVIENLLTNASKYTPKGGEVSVSVGPSGDKIHISISDTGVGIKNT
                     DMSKLFKQFSRIPNELSEHVGGSGLGLYWVNKVVQLHKGRIAVHSKVGQGSRFDIYIP
                     GGKSHGDKSTAS"
     gene            2375..3133
                     /locus_tag="VN778_04390"
     CDS             2375..3133
                     /locus_tag="VN778_04390"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HXR50237.1"
                     /translation="MKRPEVTLENYEAVYEFYGLRQPSSEMQMRVHKVLGWLYSSRIQ
                     FLDGAEAEIQEAEADDTHIVFVGTHTSMHDPFHLGGAMEREESLHWLRGRTFEPAKPI
                     LFKIPGVRQAIDSLDAVPAFRSKDAKTSDEKKLQLEATKSFLGVCVDKITNDGVMIIY
                     PKGGREKKKRDQVEGFKGGTGRIACEASKQVNVALLPVAINYRRRFFKWRNADIVFGN
                     LIHGPFENRQAVTKQSIQSLQYGLDRAIAMSKSK"
     gene            3192..>3393
                     /locus_tag="VN778_04395"
     CDS             3192..>3393
                     /locus_tag="VN778_04395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50238.1"
                     /translation="MPETRPEQSEISKRHIPIIGELTVRAARHIKQFDSLELLDPEWV
                     NLGHHIKGKPLPYKEMSEKAEEA"
BASE COUNT          884 a          765 c          812 g          932 t
ORIGIN      
        1 ctcatctatg ttgaaggcgg ctttggcgtc agtcggcaag ttaagacgca gtggtgttag
       61 cccatagaga gtgcctcgat acattttggg attacgctgt gccaaagtga gcacataaaa
      121 atctcgggca tcatgcaaca gaccgagcac cggcgctata tggaaatgat tatccacggc
      181 cacgtattct tcggtatcga ttggtaaata aaacatttcc atgacttcgg gtgctgcaca
      241 aatcaaaagg ccgggctttc cggcttccca gaaagcaagg tcatcgtgga gctcggctag
      301 ccgggcttct agggccttgg cgacttcctg gccggtacca tgcgccgaca gttgctgctt
      361 ggcgcggtga acgagatttt taaaacgaat ttgattttcg ctgatatgcg gcggtgaggc
      421 ggtggtgtgg aggggcaagt aaatagtgac tgatggctca gtttccattg aagataattt
      481 tttaagcttc tcgcgattta atttatccat atgagcatac ctccgctttg gtaacaccct
      541 tcataataag gattatctta tggtttgaga gctaattatc ttacatgttg gtaccggcaa
      601 aacaatcaag agataagcag ctagctctta ggcagcagct acttcctcgt gcaaaagtgc
      661 aaccagcccg gagggatcga gctgtgattt ttgaatgtat tttttggcgc ccagctgcag
      721 ggcttggttg atttcatcac cgtggcctag attactcagg atgatagtag ttggtagtgt
      781 cttgtatttc gcgataaatt tttttagaaa tccaatgcca tcaagctctg gcatcataat
      841 gtcaagcaga atcagatcgt atgtattggc atggcaaagt tccaaggctt tcaggccatt
      901 aacagcggtg tccacgtcga atgactcggc ctccagaatt atctggtaag actcacggag
      961 gagtttttca tcctcaacta gcagtacttt tgtccccatg gctctttccc ccaggtatat
     1021 agatgtcgaa cctagaaccc tgtcctactt tagagtggac ggcaatgcgc cccttatgta
     1081 attgtacaac tttgttgacc caatataaac ctaatccgct accaccgaca tgttccgata
     1141 attcatttgg tatacgagag aattgtttga acagcttcga catgtccgta tttttaatcc
     1201 cgacaccggt gtcactaatt gatatatgga ttttgtcacc ggagggacct acagaaactg
     1261 aaacctcacc gcctttgggt gtatatttgc tggcattagt cagaaggttt tcaataacca
     1321 tgcgtagccg tgcaggatca acttcagcga ttatcggcgc cggcggtgcc tcgaaagagc
     1381 cgtgctggct gcgcccatgg aacatatcag cgtgctcgct caaaatcagt tcaataagct
     1441 cggtaacgtc cgtcggcttt ttatggagca cgattttgcc ggcatcgacc tgcgcgactt
     1501 ttatcaggtc gttagcgatg acgatttgac gatcattgct attattggcc ttttgcaggt
     1561 aatcgatttg tgtagggttc agtgggccgc cataacccgc gagcatgatg cctaagtact
     1621 gcttgaccac tgtcgcggga ttgcgcagtt cgtgcgagac aatcgagaag aatgcttccc
     1681 gtgcccggcc cagctgctcc agcttctttt gctgcttttg aagctcccta tttgcctgct
     1741 tgagattgtc ctcagcctgc ttgcgctcgc taagatcgcg ggtaaccttg gcatagcctt
     1801 gcaaatgacc ggagttgtcg taaagagccg tgaggatgac attggcccaa aaacgatccc
     1861 cgtctttttt aagacgccaa ccttcttcct ccgtgcttcc ctgctgccgg gcagcctcca
     1921 gctcacgtct cggcttgtcg tcttttctat ctttgggtag aaaaaatacc gagaagtgct
     1981 tcccgataat ttcgttagcc ttatagcctt tcagcttctg agcaccggca ttccagctca
     2041 ttaccatgcc gtttgtgtct aggaggaaaa tcgcataatc ttgaatactt tctactagta
     2101 aacgatacaa atcagccggt tcgaccttag cctgcatggc gggattctgg ttagatgcag
     2161 ctaattttat ctgtttgact gtcttcatat ccgttacgtc tcctgttttt tgacaacaaa
     2221 gtaatacctc ggccccccgt tcaaacttct taatatacta gcattataag gagagaggaa
     2281 aggttaagat tttcacaggc acagttgctt gtaatatgtt aactctcggg gtcgtaaggc
     2341 ttgaaatatt tcaaatttag cgtacgcttt gtttatgaaa cgccccgagg tgaccttaga
     2401 aaattacgaa gctgtgtatg agttttacgg tttgcgtcag ccaagttcgg aaatgcaaat
     2461 gcgcgtgcat aaagtcctgg ggtggctgta tagctccaga atccagttct tggacggcgc
     2521 tgaggcagaa atccaagaag cagaagcgga tgatacgcat attgtctttg ttggaacgca
     2581 cactagcatg catgatccgt ttcatctcgg cggagcaatg gagcgagaag agtcactcca
     2641 ttggctaagg ggacgtactt ttgagccagc gaagccaatt ttgttcaaaa ttccaggggt
     2701 tcgtcaagct attgattcac ttgatgcggt accagctttc agatcaaagg atgcaaaaac
     2761 ttccgatgaa aagaagctgc aactagaagc tactaagagc tttcttggag tgtgcgtcga
     2821 taaaattacc aatgatggcg ttatgatcat ttatcctaaa ggtggtcgcg agaaaaagaa
     2881 acgagatcaa gtcgagggat tcaagggcgg taccggtcgt attgcttgtg aagcatctaa
     2941 acaagttaat gtagccttat tgccggtagc cattaattac cgacgtagat ttttcaaatg
     3001 gagaaatgct gacattgtat tcggcaatct catacatggt ccatttgaaa atcgccaagc
     3061 agtgacaaaa caaagcattc aaagcttaca gtacggtctc gaccgggcca ttgcaatgtc
     3121 gaagagcaaa tagctaaagt aacaaacagc cttgtgttat gcctaaactc attatatgat
     3181 tatttccaca tatgcccgaa actaggccag aacagtcaga aataagcaag cgccacattc
     3241 caattatagg cgagctgacc gtgcgagcgg caaggcatat aaagcagttc gacagtttag
     3301 aattgctcga tcctgagtgg gttaatttgg ggcatcacat caagggaaaa ccactccctt
     3361 acaaagaaat gagcgaaaag gctgaagaag cat
//
LOCUS       DATNGP010000171         7582 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_1675775, whole genome shotgun sequence.
ACCESSION   DATNGP010000171 DATNGP010000000
VERSION     DATNGP010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 7582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7582
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1675775"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(87..1217)
                     /gene="metK"
                     /locus_tag="VN778_04400"
     CDS             complement(87..1217)
                     /gene="metK"
                     /locus_tag="VN778_04400"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020156891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HXR50239.1"
                     /translation="MSNVTSFVQYFTSESVCAGHPDKICDGISDALLDAALAQDPLSR
                     TGIETVAGANKIALFGEIKTKAEINFEHIVREKVNELGYNNPAWGFSYESEFSNDVHQ
                     QSPEIAIGVDHDGAGDQGMMFGFAINETPELMPLPIALAHALTKRIDEARELREVPWL
                     RPDGKAQVTVKYEKGKPVAVEKLVLAVAHNEETNAEQVRADAIKQIITPVLERFKLEL
                     PADKNIIINGTGLWHIPGPESDAGLTGRKIVVDTYGGYARVGGGAFSGKDPSKVDRSG
                     AYAARYIAKNIVAQGLASKCEVGLAYVIGQPKPLMQTIDTFGTATISEQALHEFKDKL
                     IDTSVKGINDTLELRRPIYAQTSAYGHFGKPDLPWEQIVRLG"
     regulatory      complement(1264..1365)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(1412..1876)
                     /locus_tag="VN778_04405"
     CDS             complement(1412..1876)
                     /locus_tag="VN778_04405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50240.1"
                     /translation="MTTARQHEGNGGTTPNSQHDVYYWPSPDEEPVVGELTAHEAIQL
                     AVKATQARFRRKGVSEEAVLSKQPLHSSKPEARAEAKAAHNLLLALLNNSSVEYPDVV
                     GELKGYGSEIQVEWAAMQATRRVEYDPSLQAVFENIQKSYLKQFIGKQAIEH"
     gene            1987..2060
                     /locus_tag="VN778_04410"
     tRNA            1987..2060
                     /locus_tag="VN778_04410"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2019..2021,aa:Gln,seq:ctg)
     gene            complement(3736..4158)
                     /locus_tag="VN778_04415"
     CDS             complement(3736..4158)
                     /locus_tag="VN778_04415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50241.1"
                     /translation="MSTEGRPTVITNDTVRKLEDAFKAGFSVSEACLTAGISRATYYE
                     HRASNEAFSDKMELAKKYISIRAKKVVVQAIDDGNFNAAKWWLEHRERDQFGQNTIDE
                     DAQWAAHEASGSGGDELKQILDELKRCADLAITGAAAT"
     gene            complement(4220..5551)
                     /locus_tag="VN778_04420"
     CDS             complement(4220..5551)
                     /locus_tag="VN778_04420"
                     /inference="COORDINATES: protein motif:HMM:NF012796.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl--tRNA ligase-related protein"
                     /protein_id="HXR50242.1"
                     /translation="MKYFILSKSKEVIEVNDYLKLPKIDSEFARFVRREALGEVLVKQ
                     SNAKPKYLEPANHIGFNWEQNADIGHVQYNYKANLMRRLVQDYARQLVQGIGFPVYEV
                     NGANMFSLQHPVVSAYASLYGDRLYQFKSGKSDIVMSYDASYPQFNLASKAPLSHKQL
                     PFSHFSLSDCYRQEQSGEMMLLLRQRRFYMPDIHPYFRDAAEAFAWFPKLQAKILEAG
                     LAAKREYQVVIEVPSEKVWNEYRQFIEKIPQQLGADVLVNIIEDGKDRYWVVNVDYKI
                     IDKLGQAREIACIQVDIGNAPRLNIRYVDEDGSIKHPVIIHSAIPGGIERYLYILFDN
                     FEHGLPLWLSPVHVRLLPVGAEFVKDCQKLVEQYGSLPLRIEVDDRSISVSSKLKSAH
                     KDYVPYKVVIGQKEVADGFSELKAQIDGLVKEVEDKPFIAREWPAQLSQQM"
     gene            5903..6088
                     /locus_tag="VN778_04425"
     CDS             5903..6088
                     /locus_tag="VN778_04425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50243.1"
                     /translation="MGKRFGAKATKAFKKWLKRTKKAYALKKFKASFFLFAKRQNANV
                     RITAYWRKRKTFYLTLL"
     gene            6185..>7582
                     /locus_tag="VN778_04430"
     CDS             6185..>7582
                     /locus_tag="VN778_04430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50244.1"
                     /translation="MAGGMLAIIFTFNTLLVNFALAQYPPEFFRLSGYDKNQDKIYFA
                     IAFSSVALFVMGFMYRDLVNGWNYWLLFLGLLSIFAVLYLVFTSYILTRKRLNPIASF
                     GFITKPAFELLDQSDKDARRLADMLRKDPTLKPDKAYIADYQPHILLQSRYAQINEFI
                     GYLYDYHDKLVDKKDYSAALKVLDAIGSLILRYIDVRKSSFIMLPSEFLLVPVTDAQQ
                     FFDTNFQRMVDRATAYIRLGNQNGTRKVLYLFEGIGLKLRELKFPTPLLRQENPPFEQ
                     CVGYLSNICDAGIAQNDLEATFQVARVYAVLGSVGVSMGYVHAQSTTYDNLLKLALYA
                     AAKKQWVVMDQVAKAFTAFMQAFKAQDRAALETEFRIYMDKLGEFYWYYFAISTPSND
                     YQPNTATYMVAPLSELRQWADELARSGSNMRNAGRDSSQHDVIELADQLWRLLRDLTD
                     RKNITLANRYVATEFA"
BASE COUNT         1859 a         1822 c         1660 g         2241 t
ORIGIN      
        1 acgcgtcagc ctctcaggcg attcaatgtg tgatgagcca caccccgaca gttttcaagt
       61 acttactttg gcgggaagca ttggcgctag ccaagtctaa ctatttgctc ccacggcaaa
      121 tccggtttgc caaagtgtcc gtaagcgctg gtctgggcat aaattggccg ccgcagctcc
      181 aaggtgtcgt tgatgccctt aactgatgtg tcaatcagct tgtccttaaa ttcgtgcagc
      241 gcttgctcgc tgatagtggc cgtgccaaaa gtatcgatag tttgcatcag cggtttgggc
      301 tggccgatga cataggccag gccgacttca cacttgctgg caagcccttg cgccacaatg
      361 tttttggcga tgtaacgcgc ggcgtacgcg ccactgcggt ccaccttgct cgggtctttg
      421 ccactgaaag ctccgccgcc gacacgggcg tagccaccat aagtatcaac aacgattttg
      481 cgacccgtca ggccggcatc actctccggc cccgggatat gccacagccc ggtgccatta
      541 atgataatgt ttttgtcagc tggcagctca agcttaaaac gttccaagac gggcgtaatg
      601 atctgcttga tggcgtcagc ccgaacttgt tcagcgtttg tctcttcgtt atgtgcgaca
      661 gccagtacca gcttttcaac tgcaaccggc ttgccctttt catacttaac cgtgacttgt
      721 gccttgccgt cgggccgcag ccacggtact tctcgcagtt cgcgcgcttc gtcaatccgc
      781 ttcgtcaacg catgcgccag agcaattggg agcggcatga gctccggagt ttcattgatg
      841 gcaaagccga acatcatgcc ttggtcgcca gctccgtcat ggtcaacgcc aatggctatt
      901 tccggtgatt gttgatgcac gtcattacta aactctgact cgtaactgaa accccacgcc
      961 ggattgttat agcccagctc gttgaccttt tcgcggacaa tatgctcaaa attgatttcc
     1021 gccttagtct tgatctcacc aaatagggca attttgttcg caccggcgac ggtctcgatt
     1081 cccgtgcgac tgagtggatc ttgcgccaga gccgcatcca acagggcgtc actgataccg
     1141 tcacaaattt tgtcgggatg cccggcacag actgattcac tcgtgaaata ttggacaaag
     1201 ctagttacat ttgacattaa aaaatccttt ccccgttggc cgcctaggaa aggatatcgt
     1261 ctcaaaatat cttccccgca cgcgggtcta gatggagcac cttgctgaga ggcaggttgc
     1321 cggcggttca acgagctaga tctctccccg cactcttcat atttttaatt gttaatgctc
     1381 attagtgtag cacatcatta aattattata gttagtgctc gatagcttgc ttcccaataa
     1441 actgcttgag atagcttttt tgaatattct caaacacagc ttgtagcgaa gggtcgtatt
     1501 ctactcgtct agtagcctgc atcgccgccc attccacttg gatctccgat ccataacctt
     1561 tcagttcgcc gacaacatca ggatattcga cacttgagtt attaagaagt gccaacaaca
     1621 agttgtgagc agctttggcc tcagcccgcg cttcaggctt gctgctatgc aagggctgct
     1681 tgctaagtac cgcttcttca ctaacaccct tgcgcctaaa tcgagcttgg gtcgctttga
     1741 ccgccagctg aattgcctcg tgagcggtca gttcaccaac aaccggctct tcgtcaggcg
     1801 atggccaata ataaacatca tgctggctat tcggagttgt tcccccgttg ccttcgtgtt
     1861 gccgagcagt agtcatatgg tttactttta cttaccaaat tcgaggttgt aacagctgta
     1921 caatatctta ctaccacggt gttactggcg ccgacaacac cggtaggtta taataagttc
     1981 tcgtattggg gattggccaa gcggtaaggc actgggttct ggtcccagga tcgggggttc
     2041 gaatccctca tccccagcca agcaggattt aatacgttat accattcaga attgaacgta
     2101 ataacagata cagcacggtt agtaataacg aagtttgagt agaacttacc tagtatttcg
     2161 tctagttgtt ttaagtcctg tatctcaccg atgtaatcca ctatattttc catgagtttg
     2221 aggaactttt cctcggttac tatggactgc tttaatcgtt tcttttgctc atctatttca
     2281 gctagctcac gttcatagcg cggcaattca acctcaacat atttcattat gtcatcttga
     2341 tagtaggttg ccacttctgc cttagccggg tcagcaacta cgctcttggc cttttctaag
     2401 tcctgtttag tctgctgggc attagcacgt atgcggcgct cagcacttag cagctctatc
     2461 ttttcgtctt caatagcttg ctgtgcttca tcaacataat tggcgtaggc tttctttagg
     2521 tcaaattgcg cgctatttag tgtttcaatg gcggctgtag tgaccacatt ggcacgtatt
     2581 tggtgctttt gctgttttgg attgtcagga ttattgattt tgtactcaca gtttttatca
     2641 tcgcaccgat agtacacata agttttggcc tttgttttgc ccgtaccggt gtttaggtgc
     2701 atattgctgc cgcaatgacc acaaactatt ttgccctgca atagctcaac ctctttttgg
     2761 gcggttacca cccctctagt gactatgcgg tctaggctat ctattttatt caattttagg
     2821 aattgctctg gctctatcat ggcctcaaag ccatatagct caaccagatc aactggctta
     2881 gttttgccgt attgcaggaa acctgcataa acagtttctc tgaacatctc tgatagtttt
     2941 ttatcgttga agtgagtacg gctacgcacc tgcgcacgac cataacattg ggtatagttt
     3001 tgtttattga gccacttggc aatgtcaacg agtgataaag gctcgttttg taagcgcttt
     3061 tcaaatgctt gctgaataat gagccagttg tcgccatcag gctcaagtat tccatcgtcc
     3121 ataatcctgt agccatgcac cattttgcca agatatatac ctttatcagt acgtcgccga
     3181 tagccacgca ttacactttc acttaagtgt tcggagtatt gctttgataa tgcaaaggta
     3241 atgcctaaca tcatcttgcc gttggcgctg tcctcatact gagcagttgg aaaaagcagg
     3301 tctttgataa tgcgtttgtc cagcatatca atgatctcac cggcttcttt catgttgcgt
     3361 gctaacctgt ccgggtgcca tgcaatcaag ccctgatact taccggttcg caagtctgtt
     3421 agcatatcct taaacaattt tcgggtgtca gcttccttgg ccgagtgctc ctctttaaca
     3481 atgtcatagt tagtgatttt atgagggtca aggatttttt cttttatatc ctctagctgg
     3541 tcaccaatgg attttagttg tttatcacta ctttcatccg agctttttcg caagtaaacc
     3601 acatacttta actcttgctt gttaacttcc tgataaaggt gcccgtaacg gttctcatgt
     3661 atgtctgtga gtagcccaag caagtgtcca cgtgtcggct catctgtcat ggcctaattg
     3721 tatactagaa cacccttatg tagctgctgc gccggttatc gctaggtctg cgcaacgttt
     3781 taactcgtct aagatttgct taagttcgtc accacctgag ccgctggcct cgtgtgctgc
     3841 ccattgtgcg tcttcatcta tagtgttttg tccaaactgg tcacgctctc tatgctctag
     3901 ccaccacttg gctgcattaa agttgccatc atcaatggct tggacaacaa cctttttagc
     3961 gcgtatgctg atatactttt tagctagctc cattttgtct gaaaaggctt cgttagaggc
     4021 tctgtgttca taataggtag ctctactgat accagcggtc aaacatgcct cagacacgct
     4081 aaaaccggcc ttgaaagcgt cctctagttt acggacagtg tcattagtaa tgaccgttgg
     4141 tcttccctca gtgctcatgc actgaattat aatgctaaat aacgtttaag gctattgcac
     4201 aagcgtgatt attcaggaat tacatctgtt gactaagttg tgcaggccac tcacgtgcta
     4261 taaatggctt gtcctcaacc tctttaacaa ggccatctat ctgagccttg agctcactaa
     4321 agccatcagc aacttctttc tgtccgatta caactttata cggcacataa tctttatgtg
     4381 ctgatttgag ctttgaagac acacttatac ttctatcatc tacttcaatg cggagtggta
     4441 ggctcccata ttgctccact agcttctggc agtctttcac aaactctgct ccaaccggca
     4501 gcaaacgcac atgcaccggg ctaagccaga ggggtaatcc atgctcaaaa ttatcaaata
     4561 gtatgtagag gtagcgctct atcccacccg gaatggccga gtgaatgatg acagggtgtt
     4621 ttatactacc atcctcgtcc acatagcgaa tgtttaggcg gggcgcattg ccaatgtcta
     4681 cttgtataca ggcaatctca cgtgcttgac caagcttatc aattatcttg tagtccacgt
     4741 tcacaaccca atagcggtct ttaccatctt caatgatgtt aactaggacg tcagcgccaa
     4801 gctgctgtgg tattttttca atgaactgcc gatactcatt ccacactttc tcagacggca
     4861 cctctatcac cacttggtat tctcgttttg ctgcaaggcc agcttctaaa atcttagctt
     4921 gcaacttagg gaaccaagca aatgcttccg ccgcatccct aaaatagggg tgtatgtcgg
     4981 gcatatagaa ccggcgctgc ctaagcaaca acatcatctc accgctttgt tcctgtcggt
     5041 agcaatctga tagtgagaag tgtgaaaacg gtagctgttt gtggctgagc ggggctttgg
     5101 aagcaaggtt aaattgcggg taactagcgt cgtagctcat cacaatgtct gactttcctg
     5161 acttaaactg atatagcctg tcaccataca aagaagcgta agcgctgacg acggggtgtt
     5221 gcaagctgaa catgttagcg ccgttcactt cgtaaaccgg aaagccaatg ccctgcacta
     5281 attgacgtgc ataatcttgt actagccgcc tcatgagatt ggctttgtag ttatactgaa
     5341 cgtgcccgat gtcggcgttt tgctcccaat taaagccaat gtggtttgcg ggttcaagat
     5401 atttcggctt agcgttagac tgttttacta gcacttcgcc aagtgcttct cggcgcacga
     5461 aacgagcgaa ctcgctgtca attttgggta gctttaagta gtcatttact tcaatgactt
     5521 ctttagactt gcttaaaata aagtatttca tttgggaaac tccttggttt agatagttca
     5581 atctctgctt atcgccctaa atggagtttt gctctgagaa acaaaaaact cccgccgtcg
     5641 ggagcttttc aatcaatgac aaacccaacg gtctaggaca gtaccgttgt cgtcgtggct
     5701 tggtttgaaa tgattgaata aatcatagcc tatactatag catgttagaa tgttaaacgc
     5761 aagcgttaag cacgccttat agacgattag tgaccaccag ttggaagcaa atcaacactt
     5821 ggcacaataa aacgctaaag cttatattta catattcgct caattattgg gtaatcatct
     5881 aatttagcat acaattaagg tgatgggtaa aagatttgga gccaaggcta caaaggcttt
     5941 taaaaaatgg ttaaaacgca ccaagaaagc ctatgcctta aaaaagttca aagcgtcatt
     6001 ctttttgttt gccaaaaggc aaaatgccaa tgttcgcatc acggcttatt ggcgtaagcg
     6061 caagacattt tacctaacat tgctttaact cgttattgca gcgtctgtag caagtggtgc
     6121 ccagctctgg tttgcggaca ttaagcctga aactgctttt gggttttata cagcagccgg
     6181 ggctatggct ggtggcatgt tggcaattat cttcacgttt aatacgttgt tggtcaactt
     6241 tgctcttgcg cagtatccgc ccgagttctt taggctaagc ggttatgata aaaaccaaga
     6301 taagatttat tttgcaattg cgttctctag cgttgcttta tttgtcatgg gttttatgta
     6361 ccgtgattta gtcaatggct ggaattactg gctcctattt cttgggctac tgtcaatttt
     6421 tgccgtactc tacttggtgt ttactagcta catcttaaca agaaagcgcc ttaaccctat
     6481 cgcaagtttc gggttcataa cgaagccagc ctttgaattg cttgaccagt cagataaaga
     6541 tgcaaggcgg ctggcagata tgttgcgtaa agaccctaca ctcaagccag ataaggcata
     6601 catagctgac taccagccac acatattgct tcaatcacgt tatgctcaaa ttaatgagtt
     6661 tattggctac ctttatgatt atcatgacaa gctcgtagac aaaaaggact atagtgctgc
     6721 actcaaagta cttgatgcaa ttggtagcct tatattgcgc tacattgatg ttcgtaagag
     6781 cagcttcatt atgctgccat cagaatttct tttagttcct gttactgatg ctcagcaatt
     6841 ttttgataca aactttcagc gaatggtaga ccgagcaact gcctacataa gacttggtaa
     6901 tcaaaacggt acgcgtaaag tcctgtattt atttgagggt attggactaa agctccgtga
     6961 actgaaattc ccaaccccgc tcttacgaca agagaaccca ccatttgagc aatgcgttgg
     7021 ctatttgagc aatatatgtg acgcaggcat agcacaaaat gacctagaag ccacattcca
     7081 agttgctcgt gtgtacgcag ttctgggctc tgttggtgtc agtatgggct acgtgcacgc
     7141 acagtcaaca acctacgata acctacttaa gctagccctt tacgctgctg ctaagaagca
     7201 gtgggtagtt atggaccaag ttgccaaagc gttcactgcc tttatgcagg cttttaaagc
     7261 gcaagaccgg gcggcacttg aaaccgaatt tagaatatat atggacaagc ttggtgagtt
     7321 ctactggtac tactttgcta tatctacgcc gagtaatgac tatcagccaa atactgcaac
     7381 ttacatggtt gctccgctga gcgaactgag acagtgggcc gacgagcttg cccgtagcgg
     7441 cagtaatatg cgcaatgcag gacgcgatag ctcacagcac gatgttattg agctagcgga
     7501 ccagctatgg agattgttgc gtgatttgac cgacagaaaa aacataacac ttgccaaccg
     7561 atatgtggct acggagtttg ct
//
LOCUS       DATNGP010000172         2437 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_438479, whole genome shotgun sequence.
ACCESSION   DATNGP010000172 DATNGP010000000
VERSION     DATNGP010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2437
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_438479"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..755)
                     /locus_tag="VN778_04435"
     CDS             complement(<1..755)
                     /locus_tag="VN778_04435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50245.1"
                     /translation="MPLNHVETFQQSLVPKDQEEALIINTIYDQLAERGIARATRLQQ
                     AIASADESLASTLPSGEQFGFDTINKAFRWWHFRKAYLQDLEPIYQDMDESGDPQQHG
                     LDPRYLSYMDKLAKYNEDQDFSGSRILSGDPLYINTYADTEKERLRREMTDEELFDPF
                     ELEYDYVLRTLRKTLDAIMDSAAATGPPHERQYLTVGTGQPEYYSVIRYIEADEVKYI
                     IGLFNDEPEHTLIPAQPSESIPVETFILSYCKAR"
     gene            842..1546
                     /locus_tag="VN778_04440"
     CDS             842..1546
                     /locus_tag="VN778_04440"
                     /inference="COORDINATES: protein motif:HMM:NF017959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HXR50246.1"
                     /translation="MKQYLTRRPWLSITPVTVLAGLLYASWPLGYWLNPVANRGLASD
                     LEAAHQPYNWVFISFDIVSGLLICIASWWLLRRLRNAESRLLTYAMLGFGVFGLLTAI
                     DALLPLDCVSANVGQCRSVWHDPYFIVHGVASIGSIAGLTLSIMCVWWLAMRDYHSAK
                     HLSWVMHGAMAVWFGFGTVTFWLIMLSRSSTLAQHVFIVLCSVWVVSLPYLIWRYAFA
                     VPPHPVKQVIKKSRDL"
     gene            complement(1524..>2437)
                     /locus_tag="VN778_04445"
     CDS             complement(1524..>2437)
                     /locus_tag="VN778_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008578963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HXR50247.1"
                     /translation="NQRLRMQTILFVDEIHRFNKAQQDAFLPHVEAGIITLIGATTEN
                     PSFEVINPLLSRSRVLVLEPLSKEEIIQIVKKAASAEKLSAKVLPAKSVDLLAELSGG
                     DARIALGNLELARQLAGNKAITPEIVETAAQTRLPGYDKKGEYHYNVISAFIKSMRGS
                     EATATLYYLARMLQAGEDPKFIARRMVIFASEDIGLAAPAALNLAVSTFLAVERIGLP
                     EAQYNLFHCATVLAKAPKSREIASAMSHAITTARDYPDLPVPLHLRNAPTKLMKDLGY
                     GKGTKWEAGFEHPKGFLPAELKDLDFF"
BASE COUNT          506 a          622 c          658 g          651 t
ORIGIN      
        1 cgtgctttac aatagctcag aataaaggtt tccacgggaa ttgattcact tggctgcgcg
       61 gggatcaagg tgtgctccgg ctcatcgtta aaaaggccga tgatgtactt aacctcgtca
      121 gcttctatat aacgtatgac agaataatat tctggctggc cagtaccgac agttaggtac
      181 tgccgctcat gtggcggtcc ggtcgcggcg gcgctatcca ttatggcgtc aagcgttttg
      241 cgtagcgtcc ggagcacata gtcatactcc agctcaaagg ggtcaaatag ctcctcgtct
      301 gtcatttccc ggcgcaggcg ctctttttcc gtatcagcat acgtgttgat gtagaggggg
      361 tcaccgctca aaatgcggga accgctaaaa tcttgatcct cgttgtactt ggccaactta
      421 tccatgtaag agaggtatcg cgggtccaga ccgtgttgct gcgggtcgcc gctctcatcc
      481 atgtcttggt atatgggttc taaatcttgc aggtatgctt tgcggaaatg ccaccagcgg
      541 aaggctttat tgatggtgtc aaatccgaat tgctcgccgc tgggtagggt ggacgccagg
      601 ctttcatcgg cgctagcaat ggcttgctgc aagcgagtag ccctggctat gccgcgttcg
      661 gcaagctgat catatatggt gttaatgatc agggcctctt cctgatcttt gggcaccaac
      721 gactgctgaa acgtttccac gtgatttaac ggcacgaccc ctcctcatta aatatattat
      781 actataaagt tgctttgtta tctataaatc tgcactatta ttaacgtata atataaacag
      841 tatgaagcag tacctcaccc gccggccatg gcttagcatc acaccggtga cggtgttggc
      901 cggtttgctg tacgcttcct ggccccttgg ctactggctc aacccagtgg cgaacagggg
      961 tctagcgagc gacttagagg cggcgcacca accctacaat tgggtattta tttcattcga
     1021 tattgtcagc ggcctcttga tttgtatagc aagctggtgg ttactccggc ggctgaggaa
     1081 tgctgaatcg cgattattaa cctatgccat gctcggtttt ggcgtcttcg gactgctaac
     1141 ggcgatcgac gctttgttgc ccctcgactg tgtgtcagcg aacgttgggc agtgccggtc
     1201 ggtctggcac gacccctatt tcattgtgca tggagtggcc agcatcggct ccattgcggg
     1261 tttgacgctc agcatcatgt gcgtctggtg gctagcgatg cgggactacc atagtgccaa
     1321 acacttgagt tgggtcatgc atggcgccat ggcggtgtgg tttggctttg gcactgtcac
     1381 cttttggctc atcatgctat ctcgctctag tacgctagcg cagcatgtct ttattgtgtt
     1441 gtgcagcgtc tgggtggtaa gcctgccgta tctcatttgg cgctatgcct ttgctgtgcc
     1501 gccgcacccc gtgaagcaag ttattaaaaa aagtcgagat ctttgagctc ggctggtaaa
     1561 aaacctttgg ggtgctcaaa gccggcttcc cacttggtcc ctttgccata accgaggtct
     1621 ttcatgagtt tggtcggcgc gttgcgcaga tgcagtggta ccggcagatc ggggtagtcg
     1681 cgggcagtgg tgatcgcgtg actcatagcg ctggcaattt cgcgggattt tggcgcttta
     1741 gctagtacgg ttgcacaatg gaacagatta tattgggcct ccggcaagcc aatccgctca
     1801 acggctagaa acgtcgaaac agccaagttg agcgctgccg gcgcggccag cccgatgtct
     1861 tcggaggcaa aaatgaccat ccgccgcgca atgaatttcg gatcttcacc ggcctgtagc
     1921 atccgggcca aatagtagag tgtggccgtc gcctcggacc cgcgcatcga ttttatgaag
     1981 gcactaataa cgttgtagtg gtattcgccc tttttgtcat agccgggcag cctggtttgg
     2041 gccgcagttt cgacaatttc cggcgtaatt gctttattgc cggccagttg acgcgccagc
     2101 tctaaatttc cgagggctat ccgggcgtca ccaccggata attctgctag caaatcgacg
     2161 cttttagccg gcaagacttt tgctgacaat ttctcggcac tggccgcctt tttaacaatt
     2221 tggatgattt cttctttgct cagtggctcc agcaccaaca cgcgcgatcg gctcaaaagc
     2281 ggattaatga cctcaaaact cggattttcc gtggtcgcgc cgatcagggt gataatgccg
     2341 gcctcgacat gcggcaaaaa ggcatcctgc tgcgccttat taaaccgatg tatctcatca
     2401 acaaagagga tagtttgcat acggagccgc tgattct
//
LOCUS       DATNGP010000173         2796 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_2659469, whole genome shotgun sequence.
ACCESSION   DATNGP010000173 DATNGP010000000
VERSION     DATNGP010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2796
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2659469"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="VN778_04450"
     CDS             <1..191
                     /locus_tag="VN778_04450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50248.1"
                     /translation="NIVNNAGKLLRAADVGLKDRIARLIYLNVRVDSEKVVDYQMREP
                     FKTYFKTHKISNGRGDRT"
     gene            complement(170..245)
                     /locus_tag="VN778_04455"
     tRNA            complement(170..245)
                     /locus_tag="VN778_04455"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(210..212),aa:Pro,seq:ggg)
     gene            378..782
                     /locus_tag="VN778_04460"
     CDS             378..782
                     /locus_tag="VN778_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015262269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HXR50249.1"
                     /translation="MADRIETVREVEDPAVERSAGTTRTREITEVEPSGMTMAARVVW
                     YIAGVLLALLAFRFVLALLGANPNNGFANFIYTVSHPFVAPFFSLFGYNLHYGVSHFE
                     SYTLVGILVYAVIAWGITRLLTIGQPHQHHMA"
     gene            819..1508
                     /locus_tag="VN778_04465"
     CDS             819..1508
                     /locus_tag="VN778_04465"
                     /inference="COORDINATES: protein motif:HMM:NF024848.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase-like protein"
                     /protein_id="HXR50250.1"
                     /translation="MWQETKHGLYKQFNFENFREAFAFMTRVAELAEEYQHHPRWENE
                     WSVVQIWLITHEGGKSITDKDRQLAEAIDVIYEGQAPKVLADAVPQHDKVKIFADGGS
                     RGNPGPSASGFVVLDLEDNIIVDKGVYLGVTTNNQAEYTALKLALEECHKLGAKEVQV
                     YMDSLLVVNQMKGIFKIKNRDLWPIHDAIQQLAKGFKKISFSHVPREFNKLADAAVNR
                     ALDDHLASSDT"
     gene            1536..1907
                     /gene="rnpB"
                     /locus_tag="VN778_04470"
     ncRNA           1536..1907
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VN778_04470"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            1958..2605
                     /locus_tag="VN778_04475"
     CDS             1958..2605
                     /locus_tag="VN778_04475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50251.1"
                     /translation="MSKSYEKSTYANLKRHAAVGAVSLVAAMGPGVANAAAAQKSHHP
                     RHETPAQVEKTHLQKAAAIIEARMLRFESVAFLRGDVVIQESLQDQIDPQIKHGVSLN
                     IKNPFLVDLRHPSLQKGFKGIEVSGDYAIGFMSQTGDEPKVTLVKYDQNRMSFIPEAG
                     NAGPNIQYALFQQDKIGGLDLQNPLDSYDDLPHGGPLTDAAGNPEPIGLAYMPMK"
     gene            complement(2674..>2796)
                     /locus_tag="VN778_04480"
     CDS             complement(2674..>2796)
                     /locus_tag="VN778_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013979590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HXR50252.1"
                     /translation="YSQLIASLKQAGITLDRKVLAELAVNEPKAFTAVVKAAQK"
BASE COUNT          745 a          657 c          754 g          640 t
ORIGIN      
        1 tgaacatagt aaataacgct ggcaaactcc ttagagcagc cgatgtgggt ctaaaagaca
       61 ggattgcccg cttaatctac ttgaacgtgc gtgtagacag cgaaaaagtg gtggattatc
      121 agatgaggga gccgttcaaa acgtacttca aaacccataa aatctcgaat ggtcggggtg
      181 acaggacttg aacctgcgac ctcacggccc ccagccgtac gcgctaccaa ctgcgctaca
      241 ccccgatgaa tgacctaaaa attatagcat tattagatgt aatcccttca ggcggacggg
      301 cttttttgtg taaattgtta ctgagcccca agcaagaata gagtaatctg ttatacacgt
      361 aatccaaagg aggacgcatg gcagatcgaa ttgaaacagt tagagaagtg gaagacccgg
      421 cagtcgagcg ctcggccggt acaacgagaa cacgagaaat tacagaggtg gagccgtccg
      481 gcatgaccat ggccgcccga gtagtttggt atattgccgg ggtactcttg gcactactgg
      541 ctttccgctt tgtattggcg cttctgggag ctaaccccaa caacggcttc gcaaacttta
      601 tctacaccgt cagccacccc tttgtggcgc cgttctttag cttgttcggc tacaacttac
      661 actacggggt ttcgcatttt gagtcttaca cgctagtggg gatactcgta tatgcggtta
      721 ttgcttgggg tattactcgc ttgctgacga taggtcagcc tcatcagcat catatggcgt
      781 aagtggattg agtctcctgg catgctaaga tagtaagcat gtggcaagaa accaaacacg
      841 ggctgtacaa gcagttcaac ttcgagaatt tccgcgaagc ctttgctttc atgacgcggg
      901 tggccgagct ggccgaagag tatcagcatc atccgcgctg ggagaatgag tggagcgtgg
      961 tgcagatctg gctgattacg cacgagggcg gcaagagcat tactgacaag gaccggcagc
     1021 tggccgaagc catagatgtc atttacgaag ggcaggcgcc aaaggttctg gcggatgcgg
     1081 tgccgcaaca tgacaaagta aaaattttcg ccgatggcgg ctcgcgcggc aaccctggcc
     1141 cgagcgccag cggtttcgtc gtgctggact tggaagataa tattatcgtc gataagggcg
     1201 tttacctcgg ggtgacgact aataatcaag ccgagtacac ggccttaaag ttagctttag
     1261 aggagtgtca taagcttggg gccaaagaag tccaagtcta catggacagt ttgctggtgg
     1321 tgaaccagat gaagggcatt ttcaagatca aaaatcggga tttgtggccg atccacgacg
     1381 ccatccagca gctcgcgaag ggctttaaaa agatcagctt cagccatgtg ccgcgcgagt
     1441 tcaacaagct ggccgacgca gcggtgaacc gggcgctcga cgaccacctt gcctctagtg
     1501 acacgtagta gcataagtgt tataatagca tttggccaga ttattgattg gtcgcgtggg
     1561 cctgagtcca cgaggaaagt ccgggcagca tagggaacga cagtcgctaa cggcgaccgg
     1621 gggcaaccct agggaaagtg ccacagaaac gataccgctt aagtccagtt tttcgaaaat
     1681 ggaattaagt aagggtgaaa agagcggggt aagagcccgc agttggtcac ggtgacgtga
     1741 tcaagtggta aaccctgtcg gctgcaagga gatggatact ttgaggtcta aagcctcaag
     1801 cgtcccgcta aggattcatc gtaagaaccg ctcgagctca tgagcaatca tgagcctaga
     1861 tagatgacca gtctcgacaa aacccggctt acagataatc tggctcttta tgagacgtat
     1921 aattgacaaa atagcataat tttgctatag taattctatg agtaaatcat acgagaagtc
     1981 gacatatgcc aatcttaagc gtcacgcagc tgtcggcgca gtatcgttag ttgcagctat
     2041 gggtcctgga gttgcaaatg ccgcagccgc ccaaaaaagt caccatcctc ggcacgaaac
     2101 cccggcccaa gtagagaaga cccatctcca aaaagccgct gccatcatcg aagcgcgaat
     2161 gctacgattt gaaagtgtag cctttttgcg aggggatgtc gtgattcaag aatcgctcca
     2221 agatcagatt gatccccaga ttaagcacgg cgtttcactg aatattaaaa acccgttcct
     2281 ggtcgacctt cgccacccta gtttgcaaaa gggctttaag ggtatcgaag tgagtggcga
     2341 ctatgccatt ggctttatga gtcaaacagg tgatgagcca aaagttactt tggttaagta
     2401 cgaccaaaac cggatgtctt tcattccgga agcgggcaat gccggcccaa acatccaata
     2461 tgcactcttc cagcaggata aaataggcgg cctagacttg cagaatccgc ttgatagcta
     2521 tgatgatctg ccacatggcg gaccattaac cgatgcggca ggtaacccag agccgattgg
     2581 cttggcttac atgcccatga agtaagttaa aagttagtaa ttaaaaaagt ccctgtgtat
     2641 caaggggctt ttttaattga ttggcaacgt agtctatttc tgagcggctt tgaccacagc
     2701 ggtgaaggcc ttcggctcgt tcacggccag ttcggccagg accttgcggt cgagcgtgat
     2761 gccggcttgt ttgaggctgg cgatcagctg gctgta
//
LOCUS       DATNGP010000174         2647 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_1570093, whole genome shotgun sequence.
ACCESSION   DATNGP010000174 DATNGP010000000
VERSION     DATNGP010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2647
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1570093"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1053)
                     /gene="infB"
                     /locus_tag="VN778_04485"
     CDS             complement(<1..1053)
                     /gene="infB"
                     /locus_tag="VN778_04485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HXR50253.1"
                     /translation="MATTLQIEDNITVGALAEELKLPVTRLIGELMKNGIMATINERV
                     DFDTAQIIVGELGLDIELERKKMVAEPIKREKRVDSANATQRPPVVAVMGHVDHGKTS
                     LLDAIRGAEVAKGEAGGITQHISAYQIEHNGRAITFLDTPGHEAFAAIREHGAHLTDI
                     VIIVVAADDGIKPQTIEAIRFARNAGARIVVAINKIDKEGANEALVKQQLSEQNLLIE
                     EWGGDVVVVPVSAKTKQGIPQLLDMLLLVADVEELKADAAVPARGLIIEAHVETGRGP
                     IAHALIEEGTLKPGQFVVSGGTYAKVRNLEDTTGKPVKSAGPSAPVVISGFKTLPEFG
                     DPFTVVATEKEARSKAE"
     gene            complement(1136..2197)
                     /locus_tag="VN778_04490"
     CDS             complement(1136..2197)
                     /locus_tag="VN778_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HXR50254.1"
                     /translation="MPKSSLTMDELLAGSEVKQLETGDVIEGTVESIRKHQVWIDLGP
                     RGVGVVMRREVGHGQQLEAGQAVTVSVLDPEMEEGYALLSMRRAAKDRGWDEVQRIFD
                     ASEIVEIVPYDANRGGLLVELEGIRGFLPVSQLAAGHYPRVSGADKDEILQKLNALAG
                     KPIRVRVLDVSRKDNKLIFSEKEAVKDDMQARFAELKVGDVVEGIVTGVIDFGAFVNV
                     DGIEGLIHISEISWERVENPRDYVKVGETVKAKIIAIDKDRLSLSLKQMSEDPWLKEV
                     KQFNKGDVVEGKITRITPFGAFVQLSSSVEALVHVSEMGDDDNIDPEKIFQLNEKKEF
                     KVLDIDTDARKISLSLKRK"
     gene            2389..>2647
                     /locus_tag="VN778_04495"
     CDS             2389..>2647
                     /locus_tag="VN778_04495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50255.1"
                     /translation="MSLLSHPDSFDEVSSTSDQERSYCTMRANSPKGQISQIWTPNPG
                     KFWRAKLSLLITLASAAAMLYLHRWCVPTAVVVGASFYMYAA"
BASE COUNT          572 a          758 c          637 g          680 t
ORIGIN      
        1 ttcggcctta gatcgagctt ctttttcggt cgccaccaca gtaaaaggat caccaaactc
       61 tggcagtgtc ttaaagccac taatgactac cggcgctgac ggaccagccg atttgacagg
      121 cttgccggtc gtgtcttcca agttacgtac cttggcgtaa gtaccgcccg aaaccacaaa
      181 ttgacctggc ttcaaagtac cttcctcgat caaagcgtga gcaatggggc cgcggcccgt
      241 ttcgacgtgc gcctcaataa tcagaccccg agctggtaca gcggcatcgg ccttcagctc
      301 ttccacgtcg gcaactagca gtagcatatc aagcaactgc ggaataccct gctttgtctt
      361 ggcgctaact ggcacaacca ccacgtcacc accccactct tcaatcagca agttttgttc
      421 agacagctgc tgcttcacca gcgcctcgtt ggcgccttct ttatcaatct tgttgatagc
      481 aactacaatg cgagcaccgg cgttacgagc aaagcggatc gcctcgatag tttgtggctt
      541 gatgccatca tcggccgcca ccacaataat gacgatatca gtcagatgcg caccgtgctc
      601 acggatagcc gcaaaggctt cgtggcctgg cgtatccaaa aaggtaatcg cccggccatt
      661 gtgttcgatt tggtaggcag aaatgtgctg ggtgatgccg ccggcttcgc ctttggccac
      721 ttcggcaccg cgaatagcat ctagtagcga agttttgccg tggtccacgt ggcccataac
      781 agccaccact ggtgggcgct gagtcgcgtt agcgctatct acccgttttt cacgcttgat
      841 cggctcggcg accatctttt tgcgctccag ctcgatatcc agccctagtt cgcccacaat
      901 aatctgagcg gtgtcaaaat ctacccgctc gttaatagta gccataatcc cgtttttcat
      961 cagctcgccg attagacgcg tgacaggcag cttgagctct tcggctaatg ctccgacggt
     1021 aatgttatct tcgatttgga gtgtcgttgc cattttcctt cggagttcct ttctgcctgc
     1081 aattgtctaa agcggctagc tgatgtttac gacattatta aaacagtcgt cttatttatt
     1141 tccgcttgag cgaaagtgaa attttgcgag cgtcagtgtc gatgtcgagc accttaaact
     1201 ctttcttttc gttcagctgg aagatttttt ctgggtcgat gttgtcatcg tcgcccatct
     1261 cgctgacgtg gaccagtgct tctacgctag aagaaagctg cacaaaggca ccaaatggtg
     1321 taatgcgggt aatcttaccc tcaaccacgt cacccttgtt aaactgcttc acttccttca
     1381 gccatgggtc ttcactcatt tgcttgagag aaaggcttag gcggtctttg tcgatagcga
     1441 tgatcttagc cttcacggtc tcgccaacct tcacgtagtc acgtgggttt tccacgcgct
     1501 cccatgagat ttccgagatg tgaatcaggc cttcgatacc gtcaacattg acgaatgcgc
     1561 cgaagtcgat cacaccagta acgatgcctt caactacgtc gccgaccttc agctcggcaa
     1621 agcgagcctg catgtcgtcc ttaaccgctt ccttctcaga gaagataagc ttgttgtcct
     1681 tgcggctgac atccagcaca cgaacacgga ttggcttacc ggccagcgcg ttcagcttct
     1741 gcaggatttc gtccttgtca gcacccgata cgcgtgggta gtgaccagca gccagctgtg
     1801 ataccggcag gaagccacgg atgccttcca gttcgaccag cagaccacca cggttggcgt
     1861 cgtatggtac aatctcgacg atttcgctag cgtcaaaaat acgctgtact tcgtcccagc
     1921 cccgatcttt ggcggcgcgg cgcatgctaa gcagtgcgta gccctcttcc atttctgggt
     1981 ccagtacgct aacggttacg gcctggcctg cttccagctg ttggccatgg ccaacttcgc
     2041 ggcgcatgac cacaccgaca ccgcgcggac cgaggtcgat ccagacttgg tgcttgcgga
     2101 tgctttccac agttccttcg atgacgtcac ccgtctcgag ttgtttgact tcgctgccag
     2161 ctagcagctc gtccattgtt agtgatgatt ttggcatgtt cttactatgc ctcctttgta
     2221 gtgtatttct tgcgggaatc atacgtctgc aagcggttgt taaacacacc aaaacataag
     2281 aacccgaaag attacgtatc attataacag agaaacggag gtaaaccaat gcctgcgtca
     2341 gtgccgtgag tgagtattgc ataagtttag tatttatgcg ataatatatt gtcactgctg
     2401 agtcaccctg actcctttga tgaagtttcc tcaacttccg accaggaaag gtcgtactgc
     2461 actatgcgtg cgaacagtcc caagggccag atcagccaga tctggacacc caaccccggt
     2521 aagttctggc gagcaaagct cagcttgctc atcacgcttg ccagcgccgc cgccatgctt
     2581 tacctgcaca ggtggtgcgt tcctaccgcc gtggtcgtcg gagccagttt ctacatgtac
     2641 gccgcca
//
LOCUS       DATNGP010000175         5505 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_1416780, whole genome shotgun sequence.
ACCESSION   DATNGP010000175 DATNGP010000000
VERSION     DATNGP010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5505
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1416780"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..360)
                     /locus_tag="VN778_04500"
     CDS             complement(<1..360)
                     /locus_tag="VN778_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009482544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HXR50256.1"
                     /translation="MGDLAVHETAIPGFYEIDLAVHGDNRGWFKENYQRAKMEALGLP
                     HFEIVQNNFSYNAERGATRGLHAEPWEKYISVANGRIFGAWVDLRQGDAFGKVLTMEI
                     TPEKAVFVPRGVANGYQT"
     gene            complement(363..1364)
                     /gene="rfbB"
                     /locus_tag="VN778_04505"
     CDS             complement(363..1364)
                     /gene="rfbB"
                     /locus_tag="VN778_04505"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018768482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HXR50257.1"
                     /translation="MNKILVTGCAGFIGSNFVHYVYRERPDWHITVYDKLTYAGNRAN
                     LAGLDENRVAFIQADICDEAAMDAAVADTDAVIHFAAESHNDNSLHNPRPFLDTNIVG
                     TYTILETVRKHGKRLHHISTDEVYGDLELDDPAKFTPDTPYNPSSPYSSTKAGSDLLV
                     RAWVRSFGVEATISNCSNNYGPFQHVEKFIPRQITEVLEGRRPKLYGSGENVRDWIHT
                     EDHSSGVLTILEKGKIGETYLIGADGERNNKDVVELILELMGKDSSDYDHVNDRPGHD
                     LRYAIDASRLRNDLGWSPKFTDFREGLKATIEWYQQNENRWKPQKEATEAKYKELGR"
     gene            1436..1948
                     /locus_tag="VN778_04510"
     CDS             1436..1948
                     /locus_tag="VN778_04510"
                     /inference="COORDINATES: protein motif:HMM:NF013621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HXR50258.1"
                     /translation="MMSQEWGAKIVSFEEFEALRPNLGKVVVTSGGFDPIHPGHISCI
                     AESKKYGETLVVVVNGDAFLTAKKGRPFQNLETRSLIVSGIRGVDYVVPFEIENDQTV
                     TKALEALKPNVFTKGGDRIDKETIPEWEACQRLNIEIITGVGMDKQWSSSWFLEKWND
                     HKPAKQAKAL"
     gene            complement(1926..3272)
                     /locus_tag="VN778_04515"
     CDS             complement(1926..3272)
                     /locus_tag="VN778_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008480166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HXR50259.1"
                     /translation="MSVFDTIFKAYDIRGKVGSELNVEVVEKVGQAFGSWLPVKGPVA
                     VGRDMRPDSRQLADALIKGLRATGHDVIDIGEVTSDMIYFTTGHYGLAGGAVVTASHN
                     PGEFNGIKFCREEAKPVGEDSGLLEVRDLAKAGNFAAAERQGSLTTKDVTEDWIEHVL
                     SFINPSELTALNIAVDAGNGMAGKIFPELEPFVPFEVHEMFFDLDGTFPNHIANPLEP
                     KNLVDIQKAIKAEGCDAGVAFDGDGDRAVLLDEKGEPLSGTVMTALLAKYFLEKQPGA
                     TILCNAICGRAATETIEKNGGTAIRTRVGHSFIKGEMRKHSAIFAGEHSGHYYFKDNF
                     MADSGLLAAVIALYVLCKSKKKLSELAAPYRQSYVSITETNFEVEDKQATIEGIAKQF
                     NDGEQDNLDGLTVNYPNAWFNIRPSNTEPLLRLNAEAKTEVELDQLVAKVKALLPA"
     gene            complement(3317..5143)
                     /gene="glmS"
                     /locus_tag="VN778_04520"
     CDS             complement(3317..5143)
                     /gene="glmS"
                     /locus_tag="VN778_04520"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012780310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HXR50260.1"
                     /translation="MCGIVGYIGKRSGSPIVLSGLEALEYRGYDSAGIAVVDKQGGQV
                     IKRVGRVEGLKTAVEQQGLEAPLVIGHTRWATHGSPNMNNAHPHCNQDQTIFVVHNGI
                     IENYVELKEKLQAMGYAFASDTDTEVIPHLIDYYHKKLNTFEAAFRAALNDLCGAYAI
                     AAISADEPDTLLAARLSSPLVIGVGKDEHILASDPTALMEHTKKVVYLDDYDVATIRK
                     SKLTLHNLKQDKPTKPQIELLDYNSEQARLGDFPHFMLKEIYEAPATIRSATLGRALA
                     AQGIVKLGGLEAVEQQLKYIDRLVIVACGTSYYAGLVGEYLIEELAGIPVEVQLASEF
                     KYRDEPFSRSTALLAISQSGETADTIAALKKVEDYGVLRLGIVNAVGSTIARLTDAGV
                     YCHAGPEQAVASTKAFIAQVTVLALVALHLSNGRTKSYKPLLRELEVLPAKAQAVLDS
                     AENIKRIAEKYKDYRDFLFIGRHLTYPCALEGALKLKEVSYIHAEGYAAGEMKHGPLA
                     MIDKNFPTFALSTGSPLLEKTYSNIEEIRARSGPVVAIATKGNKAIAKLADDVMYIPE
                     TLEQTAPILTAIVMQLFAYYIAVARDLDVDRPRNLAKSVTVE"
     gene            complement(5146..>5505)
                     /locus_tag="VN778_04525"
     CDS             complement(5146..>5505)
                     /locus_tag="VN778_04525"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXR50261.1"
                     /translation="NVTFLSNVSDYDIVNHFQTALAFIMSNMDDFGIVAVEAMAAGTP
                     VIAYKKGGALDYMIPGKTGLFFERQTVSSLRVALEAAAAKSFNYEAIAEHANEFSSHV
                     FSRNVKEFIKNCLRAED"
BASE COUNT         1186 a         1376 c         1577 g         1366 t
ORIGIN      
        1 cgtctggtag ccattggcga cgccgcgggg gacaaagaca gctttttcgg gagtgatctc
       61 catggtcagg actttgccga aggcatcgcc ttggcgcaga tcaacccacg ccccgaagat
      121 gcgaccgtta gcgacggaaa tatacttttc ccaaggctca gcgtgcaggc cgcgagtggc
      181 gccacgttcg gcgttatagg aaaagttgtt ttggacgatt tcgaagtgcg gcaggccgag
      241 ggcttccatc ttggcgcgct gataattttc tttgaaccag cctcggttgt cgccgtgtac
      301 agccaggtca atttcataaa aacctggaat cgctgtttcg tggactgcca aatcgcccat
      361 acctaacgtc caagctcctt atatttagct tcggtggctt ctttttgggg cttccaccgg
      421 ttttcatttt gctggtacca ctcgatggta gctttcaagc cttcacggaa gtcggtgaac
      481 ttgggcgacc agcccagatc gttgcgtaac ctgctggcgt cgatggcata gcgcaggtcg
      541 tggcccgggc ggtcattgac atggtcgtag tcacttgagt ctttgcccat gagttctaga
      601 ataagttcga cgacatcttt gttgttcctt tcgccgtcgg caccgataag atatgtctcg
      661 ccgatcttac ccttttcgag aatggtcagc acgcccgacg aatgatcttc ggtgtggatc
      721 cagtcgcgga cattttcgcc gctgccgtag agtttcgggc ggcggccttc gagcacctcg
      781 gtgatctgac gggggatgaa tttttcgacg tgttggaatg ggccgtaatt gttggaacag
      841 ttgctgatcg tcgcctccac gccaaagctg cgaacccagg cgcggactaa taagtcagat
      901 ccggccttag tagaggagta gggactcgaa gggttgtagg gtgtgtcggg tgtgaactta
      961 gccgggtcgt caagctcgag gtcgccgtag acttcgtcgg tggagatgtg gtgcaagcgt
     1021 ttgccgtgct tgcggacggt ctccaggatg gtgtacgtac cgacgatgtt ggtgtcgagg
     1081 aaggggcggg ggttgtgcag ggaattgtcg ttatgcgatt cggcggcaaa gtgtataacg
     1141 gcatcggtgt cggcaactgc cgcatccatg gcagcttcgt cgcagatgtc ggcttggata
     1201 aaagccactc ggttttcgtc gaggccggcg aggtttgctc gattaccggc atacgtcagc
     1261 ttgtcataaa cggtaatgtg ccaatcgggc cgttcgcggt acacatagtg cacgaaattc
     1321 gaaccgatga aaccagcaca tccggtgacg agaatcttgt tcattccggt ctattatagc
     1381 tgtttatcgc ttatcagaaa atatataccc agcctgattt gctataattg agggaatgat
     1441 gagccaggaa tggggtgcaa aaattgtatc ctttgaagag ttcgaggcgc tgcggccaaa
     1501 tctgggtaag gtagtggtca ccagtggggg ctttgatccg attcatcccg ggcatattag
     1561 ctgcatcgca gaaagcaaaa agtacggcga gacgcttgta gtagtggtga atggcgatgc
     1621 atttctaact gcgaaaaagg gccggccctt ccagaatcta gaaaccagga gcctgatagt
     1681 cagcggcata agaggcgttg attacgtcgt gccatttgag atcgagaatg accaaactgt
     1741 tactaaagcg cttgaagcct taaagccaaa tgtgtttaca aagggcggag accgtatcga
     1801 caaggagacc attcccgagt gggaagcctg tcagcggctc aacatagaaa ttatcaccgg
     1861 tgtagggatg gacaaacagt ggtccagtag ctggttctta gaaaagtgga atgaccacaa
     1921 gccggctaag caggcaaaag cgctttgact ttagcgacca gttgatcgag ctcgacttcg
     1981 gttttcgctt cggcattgag gcgtagtagc ggttcggtat tgctgggccg gatattgaac
     2041 caagcattgg gatagttaac cgtcaggccg tcgaggttgt cctgctcgcc atcgttaaat
     2101 tgcttggcaa ttccctcgat agttgcctgc ttatcttcaa cttcaaaatt ggtttccgta
     2161 atcgagacat agctttgtcg atatggcgcg gccagctcac tgagcttctt tttgctcttg
     2221 cacagtacat aaagcgcaat aactgccgcc agcaggccgc tgtcagccat gaagttgtcc
     2281 ttgaaatagt agtgtccgct atgctcgccg gcgaaaatag cactgtgctt tcgcatctcg
     2341 cctttgataa aggagtgtcc cacgcgcgtg cggatggccg tacccccgtt tttctcgatt
     2401 gtttcagtgg cagcccggcc acaaatggca ttgcaaagga tggtggcgcc gggctgtttt
     2461 tctaggaaat atttggccag cagagcggtc atgacagtgc cgctgagtgg ctctcccttt
     2521 tcatcgagca ggacagcgcg gtcgccgtcg ccatcgaaag ctacgccggc gtcacagcct
     2581 tcggctttaa tcgctttctg tatatccact aaattttttg gctcaagcgg attcgcgatg
     2641 tggttcggga aggtgccgtc taggtcaaag aacatttcgt gaacctcaaa cggtacaaag
     2701 ggctccagct cggggaaaat tttaccggcc atgccgttac cggcatcgac ggctatgttg
     2761 agggcggtca gctcactggg atttatgaat gatagtacgt gctcaatcca gtcttcggtg
     2821 acatctttgg tggtgagtga tccctgtcgc tcagcagccg cgaaattacc agctttggcg
     2881 agatctcgga cttctaaaag gcccgaatct tcaccgaccg gcttcgcctc ttcgcgacag
     2941 aattttatgc cgttgaactc gcccggattg tggctggcag tcaccacggc gccaccggcc
     3001 aagccgtaat gcccggtcgt gaagtaaatc atgtcgctgg tcacttcgcc gatgtcgatg
     3061 acgtcatgac cagttgcgcg taagcctttg atgagtgcat cagccaactg cctcgaatcc
     3121 ggccgcatat cccggccgac cgcgaccgga cccttcacgg ggagccacga accgaaagct
     3181 tgtccaactt tttcaactac ctcaacgttt aattcgctgc caactttacc gcggatatca
     3241 taagctttga aaatcgtgtc gaacaccgac atggctcctc ctaaggttta tgtaatgtaa
     3301 attaacctta cttctactat tctaccgtaa ccgacttagc cagattgcgt ggccggtcga
     3361 catcgagatc gcgggcgacg gcgatgtagt aggcgaagag ctgcatgacg atggcagtca
     3421 ggatgggggc ggtttgctca agggtttcgg gaatgtacat gacgtcgtcg gcgagcttgg
     3481 caatggcttt gttgcccttg gtggcaatgg ccacgaccgg accggagcgg gcgcggattt
     3541 cttcgatgtt tgagtaggtt ttttcgagca ggggagagcc ggtggagagg gcgaaagtcg
     3601 ggaagttttt gtcgatcatg gccagcgggc cgtgcttcat ttcgccggcg gcatagccct
     3661 cggcgtggat gtaggagact tccttgagct tcaaggcgcc ttccagagca caagggtaag
     3721 taagatggcg gccgatgaac aggaagtcgc ggtaatcttt gtatttctcg gcgatccgct
     3781 tgatgttttc ggcgctatcg agcacggcct gggccttggc tggcaagact tctagctctc
     3841 tcaaaagcgg tttatatgac ttagtacgac cattgctgag gtgtaaagcg acgagtgcca
     3901 gaaccgtcac ttgggcaata aaggccttag tgctagctac ggcttgctcc ggaccggcat
     3961 ggcagtagac accggcatcg gttaggcggg cgatggtgct gccgaccgcg ttgacgatac
     4021 cgagccgtag gacaccgtaa tcttcaacct ttttgagcgc ggcaatggtg tcggcggtct
     4081 cgccggattg cgagatggcc agcagtgccg tgctgcgcga aaatggttcg tcacggtact
     4141 taaattcact agccaactgc acttcgaccg gtatgccggc tagctcttcg atcaagtatt
     4201 cacccaccaa accggcataa tatgaggtgc cacaagcgac aatgaccaga cggtcaatgt
     4261 acttcaattg ctgctcaacc gcttctaagc cgccaagctt tacgatgccc tgtgcagcca
     4321 gggcgcggcc cagtgtcgcc gaacggattg tcgccggcgc ttcatagatt tctttcaaca
     4381 taaagtgcgg gaagtcgccg aggcgggctt gctcgctgtt gtagtccagg agttcgatct
     4441 gcggcttggt tggtttgtcc tgcttcagat tatgcagggt gagctttgat ttccggatcg
     4501 tagccacgtc gtaatcatcc aggtaaacaa cttttttcgt gtgttccatg agggctgttg
     4561 ggtcggaagc caggatgtgc tcgtccttcc cgacgccaat caccaaaggg ctggagagac
     4621 gggcggccag caatgtgtcg ggctcatcgg ccgatatggc ggcgatggcg taggcgccgc
     4681 acagatcgtt caaggctgct cggaaagcgg cttcaaaggt gttgagcttt ttatggtagt
     4741 aatcaatcaa gtgtgggata acttctgtat cggtgtctga ggcgaacgcg taacccatag
     4801 cttgcaattt ttcctttagc tcgacgtagt tttcaataat gccgttatga acaacaaaaa
     4861 ttgtctggtc ctgattgcag tgcggatgtg cgttattcat gttcggcgaa ccatgggtag
     4921 cccagcgggt gtgcccgata accagcggtg cttctaatcc ttgctgctcg acggctgtct
     4981 tgagcccctc aacccgtccg acgcgcttga tgacttgacc gccttgttta tcaaccacgg
     5041 ctatccccgc cgaatcgtaa ccacgatatt caagagcttc gagtccgctc agtacgattg
     5101 ggctgccgct gcgtttcccg atgtagccga cgatcccgca cacgactagt cctccgctcg
     5161 caagcagttt ttaataaatt ctttcacatt gcggctaaat acgtgggaag aaaattcgtt
     5221 ggcgtgctcg gcgatggctt cgtagttgaa tgacttcgcc gcggcagctt ccaaggctac
     5281 gcgcaaggac gaaactgttt ggcgctcaaa gaacaggccg gttttgcctg gaatcatgta
     5341 gtccagtgcg ccaccctttt tataagcaat gactggcgtg ccggcggcca tggcttcgac
     5401 ggcgacgata ccaaagtcgt ccatgttgga catgatgaaa gccagggcgg tttggaagtg
     5461 attgacgatg tcgtaatcgc tgacattact gaggaaagtc acgtt
//
LOCUS       DATNGP010000176         5831 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_3163929, whole genome shotgun sequence.
ACCESSION   DATNGP010000176 DATNGP010000000
VERSION     DATNGP010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5831
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3163929"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1664)
                     /locus_tag="VN778_04530"
     CDS             complement(<1..1664)
                     /locus_tag="VN778_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HXR50262.1"
                     /translation="MAKAATTKAQRVYFTSVDDALDLPSLVDHQNRSFQWFIEEGLGE
                     LLAEISPIDDYTGGKLSLSFKDYHFGDPKLSEAQARENNVSFEAPLLANVELTNKVTG
                     EVKEQEIYLGDYPWMTSRGTFVINGAERVVVSQLIRSAGVFFTSELHGTSSLYGAKVI
                     PGRGAWLEFETAASGALYVKIDRKRKIAVTTLLRALGVTESQMREQFKHVDTGERNYL
                     DATLEKDPTKGQNDALIEVYRRLRPGDLATVDNAKSLIENMFYNYKRFDFSRVGRYKI
                     NKRLDLDVPNTLENRVMRLEDIEAIVAEIIRLNNTQEPVDDIDSLANRRVKLVGELVQ
                     RQFRIGLLRMERNTKDRMSMSEIETVTPGQLINARPVVAAVREFFASSQLSQFMDQIN
                     PLSELAHKRRLSSMGPGGLSRERAGFEVRDAHATHYGRICAVETPEGANIGLVLNLAN
                     FARVNDYGFIETPYRKVLNAVPAKDAVGHFAAEDLADEKGKVLIKADAKISAADAAKL
                     AKVTGKVTWPIRAKVTNEIVYLDAYEEGAAVIAGGGTEIDENGYFVT"
     gene            complement(1946..2554)
                     /locus_tag="VN778_04535"
     CDS             complement(1946..2554)
                     /locus_tag="VN778_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HXR50263.1"
                     /translation="MYKPTNQVLIPTVIENEGRYERAYDIYSRLLKDRIIFLGTDVNE
                     ATANLIVAQLLFLQAEDAKKDIFFYINSPGGSVYDALAIYDTMQYVTNDVQTVGIGVQ
                     ASAAAFLLSSGTRSKRFMLPSATAMIHQPSSGTRGKVTDQEIDLRESLRIKKLLESVM
                     AKNTGQKVEKIHEDMERDKWLTATEAKKYGLIDDVITTPPKA"
     gene            complement(2610..3113)
                     /locus_tag="VN778_04540"
     CDS             complement(2610..3113)
                     /locus_tag="VN778_04540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50264.1"
                     /translation="MYKAFGEQMIVTSGFEAQTISRALAYGLRERKGKPQPNIPIAHS
                     RESWQLPPVYEEPQFMERVTRYGELVLEAEQTALAVRNLRAIAVGATVFRNIYPKQAA
                     ALLEIYDHSDPDAVPAGPFHLAPEELTTRITAYYPTEVEARATPLELPHIDDEAIKIY
                     DARKRVA"
     gene            complement(3195..4484)
                     /gene="tig"
                     /locus_tag="VN778_04545"
     CDS             complement(3195..4484)
                     /gene="tig"
                     /locus_tag="VN778_04545"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HXR50265.1"
                     /translation="MQITQEQLSPTKVKLTITADHIALAPVKQQVVTQLGKNAKVPGF
                     RSGRAPEHLLEKQLDQSLLQTEFLEQAVNLLYVEATRNKQLRPVAQPNIQISKFVPFD
                     TLEFTAEVEVVGEITLAEYKKIKLAAKTVATVTAKEVNDVVDTLLARAGEKKAVNRAG
                     KLGDQTTIDFTGTDAKTGEPIQGADGKDYPLVLGSNSFIPGFETNLFGLKAGAEKTFD
                     VTFPKDYGVAALRAKKASFKVRVVSVEEIIKPKLDDVFAATVGPFKTVAELTADIKKQ
                     LLAEKQQRADVEYQNELLEKIANKSTVAIPDSLIDEEVDRIEDEEKRNLAYRGQTWQE
                     HLEDEGVTAEAHHAQKRPGAEVRVKTGLLLGEIAEREGVTITPEELEIRVQLLKGQYP
                     DPSMQAELDKPENRRDITSRLLTEKTLDLLKSYATSK"
     gene            complement(4538..5002)
                     /locus_tag="VN778_04550"
     CDS             complement(4538..5002)
                     /locus_tag="VN778_04550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50266.1"
                     /translation="MRAINHAITGAFIGLAISEPVAAIPVAIASHFVCDAIPHYDGVS
                     SDKGKAKWLRSKTFRYLLYADAILCGGLVLVLALKHPFDWQLAAICAFAAAAPDFLWL
                     KRYLNRHSKQQTRPNAFSRFAGRIQWFQRPIGAVVEIAWFVAGLLILAPFIR"
     gene            complement(5003..>5831)
                     /locus_tag="VN778_04555"
     CDS             complement(5003..>5831)
                     /locus_tag="VN778_04555"
                     /inference="COORDINATES: protein motif:HMM:NF024785.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HXR50267.1"
                     /translation="HEMYEIRLNSRQLTDYMLKDYLKLSEQEAHNVSKLVDRLSKMER
                     GDFIAKIDAAISPTARETGVVEKLLAILDIKDLKELPQELQKHSSVSELSSLLNTLNK
                     VGITNVLFDLALMRGFDYYTDIVFEVFDLHPDNNRSMLGGGRYDGLVGLFGVAPIPTV
                     GFGWGDVTLANFLELHHLLPDLRSETDLYVILAGDVMDAAQQPIAELRAAGLKVAVDL
                     SERKLGDQLKTADKKGIKHVLIIGEDELQSGKMKVRDLDTGQEASLPIDDLIKNMKD"
BASE COUNT         1355 a         1481 c         1428 g         1567 t
ORIGIN      
        1 gtcacgaagt agccgttttc atcaatttcc gtgccgccgc cagcgatgac ggctgcgccc
       61 tcttcgtaag catccaggta gacaatctca ttcgtaactt tagccctaat cggccaagtg
      121 accttgccag taacttttgc cagtttagcg gcatcagcgg cgctaatttt agcgtcagct
      181 ttgataagca ctttgccttt ttcatcagcc aagtcttcgg cagcaaagtg accaacggca
      241 tctttggccg gaacggcatt caatacttta cgatacgggg tttcaataaa gccgtaatca
      301 ttgacccggg caaagttagc gagattcaaa accaagccga tgttggcgcc ttccggcgtc
      361 tcgacggcac aaatacggcc gtagtgtgtc gcgtgggcat cacggacctc gaaaccggcc
      421 cgttcgcggc ttaggccacc gggacccatc gagctcaaac gacgcttatg cgctagttcc
      481 gagaggggat taatctgatc catgaactgg ctgagctgtg agctcgcaaa aaattcacgg
      541 acagcagcca ctaccgggcg ggcattgatc aattgaccag gcgtgacagt ttcgatctcg
      601 gacattgaca tgcgatcttt ggtattacgc tccatgcgca gtaaaccgat gcggaactgg
      661 cgttgcacca gctcgccgac aagcttgacg cggcgattag ctaggctatc gatgtcgtcc
      721 accggctcct gggtgttgtt caggcgaatg atctcggcca cgatggcttc gatatcctca
      781 agccgcataa cgcggttttc gagcgtgttc ggcacgtcga gatccaaacg cttgttgatc
      841 ttgtaacgac cgacacggct aaaatcaaag cgcttgtaat tgtaaaacat gttttcgatg
      901 agagacttgg cgttgtcgac agtcgccaaa tcgcctgggc gtaaacgacg atagacttcg
      961 atcagcgcat cgttctggcc cttagtaggg tctttttcaa gcgtagcgtc tagatagttt
     1021 cgctcgccgg tatcgacgtg cttgaactgc tcacgcattt ggctttcagt aacgcccaaa
     1081 gctcgcagca aagtggtgac ggcaattttg cgcttgcggt caatcttgac atacagagca
     1141 ccgctggcag ccgtctcaaa ctccagccag gcaccgcggc caggtataac tttcgcgccg
     1201 taaagactcg aagtaccatg cagctcggag gtgaagaaaa ccccagccga acggatcagc
     1261 tggctgacta ccactcgctc cgcaccgttt attacaaagg tgccccgtga ggtcatccac
     1321 ggataatcac caaggtagat ttcctgctcc ttgacttcgc ctgtgacttt gttggtcagc
     1381 tcgacatttg ccagcaaagg cgcttcaaaa ctgacgttat tctctcgagc ctgagcttcg
     1441 ctcagtttcg ggtcgccgaa atggtaatcc ttaaaactta atgataattt accgccggtg
     1501 taatcatcaa ttgggctgat ctctgccagc aattcgccga gcccttcctc gataaaccat
     1561 tgaaatgacc ggttttgatg atctaccaaa cttggtaagt cgagcgcatc atcgacacta
     1621 gtaaagtaca cgcgttgcgc tttcgtagtt gctgctttcg ccataagcgt cgcaattctc
     1681 cctcgttata tagtgattag ttctggtgat ccgtgcaggt cacaagcagc tgtgtgcatc
     1741 taaaaataca cccagcaaac ttaagcctgt tcacgcacct attgtttagg atatttgttt
     1801 cattttgtta ggtcggccga agatcctaag tcgggagttt ttcgcttgca attaattata
     1861 agctccccaa cttacactac ttcttacata ctattgtgaa gcaagagagt tttaaagtca
     1921 agaccgctaa gaagggtaag ccgctttaag ctttaggcgg cgtagtgata acgtcgtcaa
     1981 tcaggccgta cttttttgct tctgttgcag tgagccattt gtcgcgctcc atgtcttcgt
     2041 ggattttttc gactttttgg ccggtatttt tagccatgac tgattcgagg agctttttga
     2101 tgcgcagtga ctcacgtaga tcgatttctt gatctgttac cttaccccga gtgccagatg
     2161 acggttgatg gatcatagct gtagcgctgg gcaacatgaa gcgtttactc ctggtaccac
     2221 tgctgagtaa gaaagctgca gccgacgcct ggacaccaat accaactgtc tgcacatcgt
     2281 tagtcacata ttgcatagtg tcataaatag ctaaggcatc gtatacgctc ccgccgggac
     2341 tattgatata gaagaaaatg tccttcttgg cgtcttcggc ttgtaagaat aagagctggg
     2401 ccacaatgag attagccgtg gcctcattca catcagtgcc caggaaaata atgcggtcct
     2461 tgagcaagcg actatagata tcataggcgc gctcgtagcg accttcgttt tcgataactg
     2521 tgggaattaa tacttgattg gtgggcttat acatagaaag cagtataatc cgcaaattag
     2581 cactcgtcaa gcccgagtgc taattatcgc taggcaacac gttttcgcgc gtcataaatc
     2641 tttattgctt cgtcatcaat atgcggcagt tcaagcggtg tcgctctagc ttcgacctcc
     2701 gtaggataat aagcagtaat tcgagtagtc agttcttccg gggctagatg gaaaggtcca
     2761 gccggcacgg catcgggatc agagtgatca tagatctcca gtagcgctgc cgcctgctta
     2821 ggataaatat tcctaaatac ggtagctcct acggcaatag ctcgaaggtt gcgtaccgcc
     2881 aaggcggttt gctcagcctc gagtactagt tcaccatagc gggttacgcg ctccatgaac
     2941 tggggttcct cataaaccgg cggtaattgc cagctttcgc gactgtgtgc tatgggaata
     3001 ttaggctgtg gcttgccctt tctttcccgt aggccatagg ctagcgcccg agaaatggtt
     3061 tgcgcttcaa acccgctcgt cactatcatt tgctcgccaa atgctttata catgccttcc
     3121 ccttgacccc ttaagcatag ctagatcaga tcagcccgga ctgtaagtta tttaacggtg
     3181 aatgagggct acagctactt gctagtagcg tagctcttga ggagatcaag cgttttttcg
     3241 gtcagcagac ggctggtgat atcgcgccta ttctcgggtt tgtcaagttc ggcttgcata
     3301 ctcggatcgg gatactgacc cttcaagagt tgcacccgga tttctaattc ctcaggggtg
     3361 atcgtgacac cttcacgctc ggcgatttca ccgagcagca acccggtttt gacacgcact
     3421 tcggcaccgg gacgtttctg ggcgtgatgc gcttcggcag tgacgccctc gtcctcaagg
     3481 tgttcctgcc aggtttgacc gcgataagcc aggttgcgct tttcttcatc ttcgatccgg
     3541 tcaacttcct catcgatgag gctgtcgggg atagctactg tacttttatt ggcaatcttt
     3601 tccagcagct cattttggta ctcaacatcg gcacgctgct gtttttcggc tagcagttgc
     3661 tttttgatgt cggctgtaag ctcagcaacg gttttgaaag gaccgacggt ggcggcaaac
     3721 acatcgtcca gtttcggctt gatgatttct tcgacgctaa ccacccggac tttgaacgaa
     3781 gccttttttg ctcgtaaggc agccacgccg tagtctttag ggaatgtgac gtcaaaagtc
     3841 ttttcggcgc cggcttttag tccgaagaga ttggtttcaa aacccggtat aaaactattg
     3901 ctaccaagta ccagcgggta gtccttacca tcggcgccct gaatcggttc gccggtcttg
     3961 gcatcagtcc cggtaaaatc aatggttgtt tggtcgccca gcttaccggc tcgatttacc
     4021 gcttttttct ctccagctcg agccagaaga gtatccacga cgtcgttgac ttctttggcg
     4081 gttactgtgg cgacagtctt ggcggccagt ttgatctttt tatactcggc cagtgtgatt
     4141 tcacccacca cctcaacttc ggcagtaaac tcaagtgtat caaaaggaac aaatttgcta
     4201 atttgaatat tcggttgcgc taccgggcgc agctgtttgt ttcgggttgc ctcgacatag
     4261 agcaggttga ctgcttgctc cagaaattca gtttgcagca gggactgatc gagctgcttc
     4321 tcgaggagat gttcgggagc tctgcccgaa cgaaaacccg ggactttggc atttttaccg
     4381 agctgcgtca ctacctgctg cttcacggga gcgagtgcaa tgtgatccgc agtaatagtt
     4441 aatttgacct tcgtcgggct aagctgttct tgtgtaatct gcataagggg ttagtgtacc
     4501 agttttatgg taatttgaat agacataccg tatagactta ccgtataaaa ggcgcgagta
     4561 taagcaggcc ggctacaaac caggcgattt cgactaccgc gccaataggt ctttgaaacc
     4621 actggatacg accggcgaac ctgctaaaag cattcggcct ggtttgctgc ttggagtgac
     4681 gattgagata tcttttcagc cacagaaaat ccggcgctgc tgccgcaaac gcacatatgg
     4741 cggccagttg ccaatcaaac ggatgcttga gtgctagcac gagcactaaa cctccgcaca
     4801 aaatggcatc ggcgtacagc aagtaacgaa acgttttgct gcgcaaccac ttcgctttac
     4861 ctttatcgga acttacaccg tcgtaatgtg gtatggcatc acagacaaag tgtgaggcta
     4921 tggccacggg tatggctgca accggttcgc taattgctag tccgatgaaa gctccggtta
     4981 tagcatgatt aatagctctc atttaatcct tcatgttttt tataagatca tcgatcggga
     5041 gggaggcttc ctggccggtg tccaggtctc taactttcat tttaccactt tgcagctcgt
     5101 cttcgccaat aattaaaacg tgtttgatac cttttttgtc agccgtttta agctgatcac
     5161 caagcttccg ctccgacaga tcgaccgcca ccttgaggcc tgcagcccgg agctcggcaa
     5221 taggttgctg tgccgcatcc ataacgtcac cggcgagtat tacataaagg tcagtttctg
     5281 accgtaagtc gggcaataaa tgatgtaact cgagaaagtt agctagtgtt acatcacccc
     5341 acccaaagcc tactgtcggt atcggggcga cgccaaacaa gcccaccaag ccgtcgtagc
     5401 gaccaccgcc cagcattgag cggttgttgt cgggatgcag atcaaagact tcaaaaacaa
     5461 tgtccgtgta gtaatcaaaa ccgcgcatta gggctaggtc aaagagcaca ttcgttatgc
     5521 ctaccttgtt caacgtatta agaagactgc tcagctcgct aacggaagag tgcttttgca
     5581 gttcttgcgg cagctcctta aggtctttga tatcgagtat ggctaacagt ttttcgacga
     5641 ccccggtctc ccgggcagtt gggcttatcg ccgcgtcaat tttagcgata aaatccccgc
     5701 gctccatttt tgataggcgg tcaactagtt tgctgacatt gtgagcttct tgctcgctca
     5761 attttaaata atccttaagc atgtagtcgg ttaattggcg gctattgagc cgaatttcat
     5821 acatttcatg c
//
LOCUS       DATNGP010000177         8072 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_1374236, whole genome shotgun sequence.
ACCESSION   DATNGP010000177 DATNGP010000000
VERSION     DATNGP010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 8072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8072
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1374236"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(88..759)
                     /locus_tag="VN778_04560"
     CDS             complement(88..759)
                     /locus_tag="VN778_04560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50268.1"
                     /translation="MYYRTYQENLVLIEHLERVHKGFKSNLGVQNKSRKWAVWQLEGA
                     LLGLAFRAVQYQKEEKDMLPIAMLLRLASEYLNQVAYFANPNTKADTIRQWYKVYYYS
                     SNLEDILYTGFRRPSAKDRHAKSKTLPEGIDFSLGEYQHYKEVNAILSLWMHPSFDLT
                     RRIFETDRNNGSYDLYNFRDTTYIDTVAGFETKQMINCAAQSLHIGSYLFGPPIALSD
                     ILAIE"
     gene            866..1078
                     /locus_tag="VN778_04565"
     CDS             866..1078
                     /locus_tag="VN778_04565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50269.1"
                     /translation="MAKKRRKRKQKLAPIPIATHGSVRVRGIKRKEIDEEKLALAFIL
                     LAQDIGKEKAADKKDLDEPRPTSQTG"
     gene            1238..1696
                     /locus_tag="VN778_04570"
     CDS             1238..1696
                     /locus_tag="VN778_04570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50270.1"
                     /translation="MSKSRETNPPQPEAEFPEVFEVTPEALQQSIDYVRANLRASFQA
                     AKEAQARGNTASGRIEQTTANTLRDKALGGMELLGYLLAPPAGAVNWHEIISDENFRV
                     DRVAEIGASGDEAADTSNEPSWEQQVARASVDRSVTGIGKDSAQRLMWGD"
     gene            1750..2082
                     /locus_tag="VN778_04575"
     CDS             1750..2082
                     /locus_tag="VN778_04575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50271.1"
                     /translation="MIGFVLLISSLLGAVIFGLLLYSAVTILRRFASKKIPVEEKKGM
                     ARRLLIALTVVVVPLSLYAAITYWHSHTKNQADNNVTSCPVGEILAQSAATGKNFCVN
                     ENYQGTTD"
     gene            2145..2369
                     /locus_tag="VN778_04580"
     CDS             2145..2369
                     /locus_tag="VN778_04580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50272.1"
                     /translation="MNSVSARIVLVKLLIAGDVLFILWILYNGMDSGWQATPLQLVVY
                     IALIALLSLSSIFLYSYSKSLGIKHLDKQQ"
     gene            2438..2821
                     /locus_tag="VN778_04585"
     CDS             2438..2821
                     /locus_tag="VN778_04585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50273.1"
                     /translation="MTEKIKTIEPSAGIAGPAAEAANIAAHESVAPTEALRLAGTIDA
                     GSLALEHSVEAPAEVATPSKLRRGRRMLATVFSTLEARGLSPVKLKDDLKFGFDLVTT
                     DPHEQGEAAYISRKEAAPRPEVRRA"
     gene            2850..3119
                     /locus_tag="VN778_04590"
     CDS             2850..3119
                     /locus_tag="VN778_04590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50274.1"
                     /translation="MSASGWQRFKRELIVGIGSMIFLLGIFGAIILTLSKVEHQKALW
                     VSVSLVGALIGWAMVRTQSRDFFDTFKQMLSQLLSAIFNPINWFN"
     gene            3123..3848
                     /locus_tag="VN778_04595"
     CDS             3123..3848
                     /locus_tag="VN778_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013735049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXR50275.1"
                     /translation="MEAAIKIKNVSVVLGGHFTALDDVSVELPEGQIVGFIGPSGAGK
                     TTLIRAIVGRQKITAGSIMIFGQPAGSARLRPQLSYMTQAVSAYADLTVQQNLHYFAT
                     MFGIKRKDVGSVVTEILQVVGLTPQTKQLVGKLSGGQKQRVSLAIALIGYPKLMVLDE
                     PTVGLDPVLRDELWKLFRKLASGGTTIIVSSHVMDEAERCDDLLLIREGKILAHDAPE
                     ALCAKTHSKTVEESFLKLVGAAK"
     gene            3845..4579
                     /locus_tag="VN778_04600"
     CDS             3845..4579
                     /locus_tag="VN778_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015034400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXR50276.1"
                     /translation="MSLHKTLATAQRVLQQLSHDPRTLALVLLVPAILLTILKFVFQG
                     NIFLFDNLAPMILGIFPLIMMFIITSVATLRERTIGTLDRLMTYPMSKLDFIFGYAIA
                     FAVLALLQAIIASGVVLGFLGVTVLGGTLPLILSAVVAALLGTALGLFMSAFATSEFQ
                     AVQFMPAFIFPQLLTCGLFVARDHMAKVLQWFADVMPLTYGVDAMKQVTAYAGWTGVL
                     SRDLLVVIGYGIVALILGSITIRRQE"
     gene            4694..5383
                     /locus_tag="VN778_04605"
     CDS             4694..5383
                     /locus_tag="VN778_04605"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXR50277.1"
                     /translation="MHTNHSKESIKLLIVEDNESLVSRLQNYMNRQFALDVVHTAADA
                     IKQIGSRDYDIIVLDLGLPDDSGEAVCRVARDKGITTPILILTGENAVDSKVRLLETG
                     ADDYLTKPFKLQELQARLGALLRRTKGAPYILVLGDLTIDLEGRRVKRGNKEIMLRRK
                     EFDILQYLALNRGRIITRPMIMEHVWDEDKENWNNIVDVHIKHLRDKVDRPFKTPLIK
                     TAHGLGYMLDA"
     gene            5627..6973
                     /locus_tag="VN778_04610"
     CDS             5627..6973
                     /locus_tag="VN778_04610"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HXR50278.1"
                     /translation="MPLSTDQNVQAREFVLNSLLTGSVFFVSFAFLIILLVSWLVLHH
                     AYVVTYFPALFGSLFFFLTLYAMARKGKYRPAATLLIIGYSVPAVLLVRQWGILVPES
                     ILLFALVIVLSGILLGSKYAFINSLLVTVVLTVFLVLQFDHVVVPDISWGKQYGTVAD
                     VLGFAVTFTVIGLVSWLYNWQMERSLTRARRSERALQRQKGLLEVTVERRTRQLQAAQ
                     AEKMKQLYHFAELGHVSTALLHDLSNHLAMLTLDIESLGSSHSNRPGVVKQAQKSITY
                     VEELVQRVSYQLQGEAIVRDFSIVEEVSESMQLLAYKAKRAQVALELIVNPKQGTYQY
                     SGDFVGFRQLVSNILANGIDAYSDTTRAPKQVRIEVTRTAADYIITITDRGKGIKASD
                     LGRIFNPFFSTREEGMGIGLFIARQLTEEQFKGHLTVTSKPNVGTTFTIQLPRKRK"
     gene            6977..>8072
                     /locus_tag="VN778_04615"
     CDS             6977..>8072
                     /locus_tag="VN778_04615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HXR50279.1"
                     /translation="MSVAPHTPPAKARALRFIASQQQADGSFISFSSPSLAPFTKKHA
                     YTTVFTPAIILGSLTGLEDETAAKVRERLAEWLIRQKDTSWSFNYWAVEAPERKSLPF
                     PDDLDDTFCALIGLYLHDQSLLGGSALAHIVKLLIATETTAGGPYRTWLVPKKSEAAW
                     LDVDLAVNCNIGYFLSLVSNPLPNVSKLLTRAVSAKRFDSPYYPSEYPVLYYVARAYQ
                     GPKRVTLVQYILGRRQADGSWGTPLQTALMVSALCNLGHATDLESAKTYLLARQSQNG
                     SWEAEAFCLDPAQGQHQYFSGAPALTTALVLEALQKLEQAEASNKTRAAGSLDKRASK
                     IYEAVMTATQKQFSELGHEVSTGALALLERL"
BASE COUNT         1972 a         1921 c         2141 g         2038 t
ORIGIN      
        1 agcgccacaa cgtgatggcg cttttttgtg tgtcgtgcta gataacagag gcaaaagtgg
       61 gtttgaatcc caccaggtcc gccaaggtta ctcgatcgct agtatgtcac ttaacgcaat
      121 cggcggtcca aatagatatg aaccaatgtg cagcgactgg gcagcacagt taatcatctg
      181 ctttgtttcg aaaccagcga ccgtgtcgat ataggtagtg tctctgaaat tgtacagatc
      241 gtagctgccg ttatttctgt cggtttcaaa tatacgtcga gtcagatcaa agctagggtg
      301 catccaaagg gacaaaatgg catttacttc cttgtagtgc tggtactcgc cgagcgaaaa
      361 gtctatgcct tcaggtagag tttttgattt agcgtggcga tcctttgctg atggcctcct
      421 aaatcctgta taaagtatgt cctctaaatt cgaggaatag taatacacct tgtaccactg
      481 tctaatagtg tcggcttttg tgtttgggtt ggcaaaatag gccacttggt tcaagtattc
      541 agaagctaag cgaagaagca tcgcaattgg gagcatatct ttctcttctt tttggtattg
      601 aaccgctcgg aacgctagcc cgagtaatgc gccctctagt tgccatacag cccatttgcg
      661 gcttttattc tgtactccga gattggactt gaaccctttg tgtacgcgtt ctaggtgttc
      721 tatgagaacc aaattttcct gatatgttct gtaatacata cactaaggtt tagttttctc
      781 ctccatctat ttatagataa gtcgcgacga aaaatacagg gggttcccaa gcctcctctc
      841 agctccccca ggcatagtgg ggtgcatggc caagaagcga cggaagcgga agcagaaatt
      901 ggcgcctata cccatagcaa cacatggttc ggttcgtgtt aggggtatca agcgcaagga
      961 aatagacgag gagaaactag cactcgcctt tatattgcta gcccaggaca tcggcaaaga
     1021 aaaggcagct gacaagaaag acctcgatga gcctaggcct acatcacaaa ctggctgaca
     1081 gctttttgag gagattctgg cgcagcgcca gacccgtagg ctcaatgaga tgttcgagaa
     1141 gagtctcaag cggtgattga gggtttgccg gtagtcccgg gagctgtacc acatccctta
     1201 tgcttgcaat gcataaaaag tagaacataa ttgtgatatg tcaaaaagtc gagagacgaa
     1261 tccaccacag cctgaggctg agttcccaga agtgtttgag gttacgcctg aggcactcca
     1321 gcaaagcata gactatgtaa gagctaatct tcgagcttct tttcaagctg caaaggaagc
     1381 gcaagcgcgt ggcaacaccg cgtcagggcg tattgagcag accacagcaa ataccctgcg
     1441 tgataaagct ctgggaggca tggagttgct cggttatttg ctagcgcccc cggctggtgc
     1501 tgtgaattgg cacgaaatta tttctgacga gaactttcgc gttgatcgag tagctgagat
     1561 tggtgcttca ggtgatgagg ccgccgacac gtcaaacgaa cccagctggg agcagcaagt
     1621 cgctcgtgcc tctgtggacc gaagcgtcac aggcatcggc aaggattcgg ctcaacgctt
     1681 gatgtggggc gattagttgc gttgagccac atatatcact accaaaacta tccgtgctaa
     1741 acttcagctg tgatcggttt tgtattatta atatcatcct tgcttggcgc agttatattt
     1801 ggcctgcttc tctatagcgc tgtcaccatt ttacgaaggt ttgcgtccaa gaaaataccg
     1861 gtggaagaga agaaaggtat ggcaaggcga ttactgattg cgcttaccgt agtagttgtt
     1921 ccgttaagtc tttacgctgc tattacatat tggcatagtc atacaaaaaa ccaggccgat
     1981 aataacgtca cgagctgccc agtaggtgaa atccttgccc aaagtgccgc taccggcaaa
     2041 aatttctgcg taaatgaaaa ttaccaaggc acaacggact aaacaagctt gtttatagga
     2101 gtttgaactt atcgggtggt acttatgagc gtatacttta aagtatgaat agcgttagtg
     2161 cacggatcgt tttagtcaaa cttttgatag ccggggatgt gttattcatt ctctggattc
     2221 tgtataacgg catggattcc ggctggcagg ctacgccctt gcagttggtt gtgtatattg
     2281 cgctgatcgc actgctttct ctaagcagca tctttttata ttcatactcc aaaagtttgg
     2341 gtattaagca cctcgataag cagcagtagg gttcagctct cctcaaggca cgcatatcat
     2401 aattgacatt ttatcaagct tatgctaata tatgtgcatg acagaaaaaa taaaaactat
     2461 tgagccttca gctggcattg ccggtcctgc cgctgaggcg gctaacatcg cagctcatga
     2521 gtctgttgct ccaactgagg ccttacggct ggctggcaca attgatgcag gcagcttggc
     2581 gcttgagcat agcgttgagg ccccagccga agtagcaaca cccagcaaac tccgtagagg
     2641 ccggagaatg ctggccactg ttttctcgac acttgaagcc agagggttat cgccagtcaa
     2701 actgaaagac gatttgaagt ttgggtttga tttagttacc accgatcctc atgagcaggg
     2761 cgaggcagct tacattagcc ggaaggaagc agcgccgcgg ccagaagtta ggcgagccta
     2821 aattcttaca cctttgttat agttaagtta tgtcggcttc cggatggcaa cgctttaagc
     2881 gtgaacttat agtaggtatc ggtagcatga tttttctgct gggaattttc ggagccatta
     2941 tattgacact cagcaaggtc gagcatcaga aagcgctttg ggtttccgta agcctagttg
     3001 gcgcattgat aggttgggca atggtgcgaa cccagagccg ggatttcttt gatacgttta
     3061 aacaaatgct gagccagctg ctgtcagcaa tatttaaccc tataaactgg ttcaattgac
     3121 gtatggaagc agctataaaa atcaaaaacg tatcggtggt gctaggcggg cattttacgg
     3181 cgctcgacga tgtaagcgtt gagctgccgg aggggcagat tgtgggcttt attgggccgt
     3241 caggtgctgg taaaacaacg cttatccggg caattgtcgg tagacagaaa atcaccgccg
     3301 gcagcatcat gatcttcggt cagccggccg gctcggcgcg cttgcggccg cagctcagct
     3361 atatgacaca agcggtttcg gcgtatgctg atctgactgt gcaacagaac ctacactact
     3421 tcgcgaccat gtttggcatc aagcgcaagg atgttgggtc ggttgtgaca gagattttac
     3481 aagttgtggg cctgacccct cagaccaagc aattggtcgg taagctttca ggtggccaaa
     3541 aacagcgcgt gtcgttggct atcgccctca tcggctatcc gaaactcatg gtacttgatg
     3601 agccgacggt cggccttgat ccggtgctgc gggatgagct ctggaaactg ttccgtaagc
     3661 tggcaagtgg cggcacgact atcatcgtgt ccagccatgt catggatgaa gccgagcgct
     3721 gcgacgattt gctgctgatc cgcgagggca aaatcctggc gcatgacgcc cctgaagcgt
     3781 tgtgcgcgaa aactcactcc aagaccgtcg aagaaagctt tttgaagctg gtgggggcgg
     3841 ccaaatgagc ttgcataaaa ccttggcgac agcgcagcgc gtgctgcaac agctttccca
     3901 cgatccgcgc acgctagcgc tggttctgct ggtgccggcc atcctcctta ccattcttaa
     3961 atttgtcttc cagggcaaca tttttctctt cgacaacctg gcgccgatga ttctggggat
     4021 ttttccgtta attatgatgt ttattattac ctctgtagca accttgcgcg agcggactat
     4081 tggcacgctt gatcggctaa tgacctaccc tatgtccaag ctcgatttca tctttggtta
     4141 cgctatcgcc tttgcggttc tggcactcct gcaggcaatt attgcgagcg gggttgtttt
     4201 ggggttctta ggcgttactg tcttgggcgg gacgttgccg ctgatactga gcgcggtggt
     4261 agcggcgctg ctcggtacgg cgctcgggtt attcatgagc gcttttgcca ccagcgaatt
     4321 tcaggccgtg cagttcatgc cggcctttat ttttccgcaa ctgctgacct gcggtctgtt
     4381 tgtagcccgc gaccatatgg ccaaggtgct gcaatggttc gcggacgtca tgccgctcac
     4441 ctacggcgtc gatgccatga aacaagtcac cgcttatgcc ggttggacgg gagtattgag
     4501 ccgagatctg ctggttgtta tcggttacgg tatagtggct ttgattttgg gctccatcac
     4561 catccgccgc caagaataaa gagtctgtta ttaaaacatt gcgaactttt gtcccactta
     4621 cccagaaaat aactaaaaag ttaataatta tttaataatt gagccgtaca ctagaggtgg
     4681 gaaaggcgct gctatgcaca cgaatcattc taaagagtcc atcaaactcc ttatcgtcga
     4741 agataatgag tcactggtgt cgcggctgca aaactacatg aatcggcaat ttgcgctgga
     4801 cgttgtgcat accgccgctg atgctattaa gcaaatcggg tctagggact atgacatcat
     4861 tgtgcttgac ttgggtctgc cggacgacag tggcgaagca gtgtgccgtg ttgctcgcga
     4921 taagggcata acgacaccca ttcttatctt gaccggggaa aacgccgtgg attccaaggt
     4981 gcgcttgctg gaaaccggtg ccgacgatta cctgacaaag ccgtttaagc ttcaggagct
     5041 gcaagcccgg ctcggcgcct tgctcaggcg gacaaagggt gcgccctaca tacttgtcct
     5101 tggcgatttg accatcgacc tggaaggccg tcgcgttaag cgtggcaata aagaaattat
     5161 gctgaggcgc aaggagttcg acatcttgca atacctggcg ctgaaccgcg ggcgcatcat
     5221 aacgcgcccg atgatcatgg aacacgtgtg ggatgaggac aaggaaaact ggaacaatat
     5281 cgtcgacgtc catatcaagc atcttcgaga taaagttgac cggccgttta agacgccact
     5341 aattaaaacc gctcatggac tgggctatat gctcgatgcg taataaaata tataagaaag
     5401 gagggggcca tgaagaacgt aattaccagc atactgaccg ataccagcgt acgcagtgat
     5461 actgcgatag aacaaaacct ggttcatcag gctgagatcg ccgcgccctg gacagcagct
     5521 atctaaccgg accaagaaca ctaataagcg taagggtcgg tgatggacga ccttgttgac
     5581 atgtgcttga agcgcgatga taaaaaaatt ctatacaaaa ttcctaatgc ccttgtcgac
     5641 tgaccaaaac gtgcaagccc gcgagtttgt tttgaattca ctactcacgg gcagtgtgtt
     5701 ttttgtgtct ttcgcttttc ttataatttt gttggtttct tggctcgttc ttcaccatgc
     5761 ttatgtggtc acctactttc cggcgctatt cggcagccta ttcttttttc ttacgctcta
     5821 cgctatggcc cgaaagggga aatatcggcc ggcggccact ttactcatca taggctatag
     5881 cgtacccgct gtgctgctag tgcggcaatg gggcatcttg gtacctgaaa gtattttgct
     5941 attcgcgctc gtcattgtcc tgtcaggcat cttgctcggt tcgaagtacg cctttataaa
     6001 cagcctgcta gtcaccgttg ttttgactgt tttcttagtg ctgcaattcg accatgtcgt
     6061 ggtgcccgac atctcgtggg gcaagcagta cgggaccgtt gccgatgtgt tggggtttgc
     6121 tgtaactttt acggtgatcg gtttggtgag ctggctgtac aactggcaaa tggagcggtc
     6181 gctcacgcga gcccggcggt ccgaacgggc cctacagcgc cagaagggac tcctggaagt
     6241 aacagtagaa agacgcaccc ggcagctaca agccgctcaa gccgagaaaa tgaagcagct
     6301 ctatcatttc gctgagctcg gccacgtcag cacagcgctt ttgcacgact tgtcgaacca
     6361 cctcgccatg ctaacactgg atattgagag tttgggcagc tcgcatagca atcggcctgg
     6421 ggtcgttaag caagcccaaa agagtattac gtacgtcgag gaactcgtgc agcgcgtaag
     6481 ctatcagctg cagggcgaag ccatcgtgcg ggatttcagt attgttgagg aagtctctga
     6541 gtctatgcaa ttactggcgt ataaggcaaa acgagcgcag gtggcacttg aattgatcgt
     6601 taacccgaag caaggaactt atcagtattc gggcgatttt gttggctttc gccagctagt
     6661 cagcaatata ttggcaaatg ggatcgatgc ctatagcgac acgactcgtg cgccaaaaca
     6721 ggtacgtatc gaggtcacga ggacggctgc agattatatt attactatta cggaccgggg
     6781 caagggaatt aaggcgagcg atttgggcag aatatttaac cccttcttta gtacgcggga
     6841 agagggtatg ggcataggcc tttttatcgc cagacagctg acagaagagc agttcaaagg
     6901 ccatctcact gtaaccagca agccgaatgt cggtaccaca ttcactattc agctgccgag
     6961 aaagcgaaag taaacgatga gtgtcgcgcc acacaccccg ccagcgaaag cccgggcatt
     7021 gcggtttatc gcttcacaac aacaagcgga tggcagtttt atcagttttt ccagcccgag
     7081 tctggcgcca ttcaccaaaa aacatgcgta cactaccgta tttacgccag ctatcatact
     7141 cggaagctta actggactcg aggacgaaac ggccgcgaaa gtccgcgagc ggttggcgga
     7201 atggttgatt cggcaaaaag atacgagctg gtcgttcaat tattgggccg tggaggcacc
     7261 cgagcggaaa tccctgccgt ttcctgacga cttggacgac accttttgtg cccttatcgg
     7321 cttatacctg cacgatcaat cactgcttgg cggcagtgca ctggctcata ttgtcaaact
     7381 gctgattgcc acggaaacga cggccggcgg gccgtatcgt acctggctgg taccgaagaa
     7441 gagtgaggcg gcatggctgg atgttgattt ggcggttaat tgcaatattg gctactttct
     7501 gtctctggtt agcaaccctt tgccgaatgt gagcaagtta ctaacgaggg ccgttagtgc
     7561 caagaggttc gattcgccgt attatccgtc tgaatatccg gtcttgtact atgtggcgcg
     7621 agcttatcag gggccaaaac gggtgacgct cgtgcagtat atactggggc ggcgacaagc
     7681 ggatggatcg tggggaacac ctctgcaaac agcacttatg gtttcagcct tgtgcaacct
     7741 ggggcatgct accgatttag agtccgcaaa gacgtatctg ctagcccgtc agagtcagaa
     7801 tggcagttgg gaggccgaag ctttttgtct ggatccggct caaggccagc accaatactt
     7861 tagcggtgcg ccggcactga ccaccgcttt ggtattggaa gctctgcaga aactagagca
     7921 ggcagaggcc agcaataaga ctcgcgctgc cggctccctc gataagcgag catcaaaaat
     7981 ctacgaagcg gtcatgacag ctacccagaa gcaattttca gagcttggtc acgaagtgag
     8041 caccggtgcg ctggcactcc tcgaacgttt ga
//
LOCUS       DATNGP010000178         4364 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_802876, whole genome shotgun sequence.
ACCESSION   DATNGP010000178 DATNGP010000000
VERSION     DATNGP010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4364
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_802876"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            258..1427
                     /locus_tag="VN778_04620"
     CDS             258..1427
                     /locus_tag="VN778_04620"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HXR50280.1"
                     /translation="MGILLFVIGILLFIGLVVVHEWGHFITARRNGVEAEEFGIFFPP
                     RLYSRKTKAGWVFSINALPLGGFVKLKGEHDSDTERGSFGAASTWAKSKIMAAGVIMN
                     LVTALVLLTILALIGMPKLIPNQYTVKRDTQLSSQSVIIGYVEPGSPAQKAGLQPEDE
                     LTDIGLRGYSPVAVNNADKLPTVTKDFAGKKVTIYYERAGQHHEAEATLRTTRVVEAS
                     QKTNNPIGYLGISPTEFTVQRSTWSAPLVAVGLSAQVTGLTLQGLGHALGGLGSLIAG
                     AVTGNTTARQNGQASATSQVAGPVGIFVILKDGSLLGYQFMLFIIAIISLTLAIMNIL
                     PIPALDGVRLWLMLLTRALKRPLTPKKEELINAAGFVVLIGLVILVTIVDVQRFF"
     gene            1408..2178
                     /locus_tag="VN778_04625"
     CDS             1408..2178
                     /locus_tag="VN778_04625"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HXR50281.1"
                     /translation="MSKDSSSPTIARVPWNALGGVIFLIVIFYVSQFFAGILISIYPG
                     LKHWTDAQANSWLDNSVTAQFIYILLAESFAVGAIYLFLRRYKANFSLIGLRRPRWRD
                     LGYGLMAAPLYYGLYFVAVGVVTHYVKGLNANQQQQIGFTNVHGSPQLIMTFISLVVL
                     PPIAEEIMVRGFLYSTLRKALPMISAVLLTSAIFASAHLPEGGAAGPLWIAALDTFVL
                     SLFLIYLREVTGSLWASIVLHATKNGIAFAALFALHVK"
     gene            2214..3476
                     /locus_tag="VN778_04630"
     CDS             2214..3476
                     /locus_tag="VN778_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl--tRNA ligase-related protein"
                     /protein_id="HXR50282.1"
                     /translation="MKLSQLFTKTTKNVPADETARNAQLLIRAGFIHKEMAGVYAYLP
                     LGLRVLENIKQIVREEMNGLGSQELIMTSLQRQELWEITDRWDDKQVDIWFKTQLQNG
                     TPIGLAWSHEEPITMMMKNYIASYRDLPTGVYQFQTKLRNELRAKSGIMRGREFVMKD
                     LYSYARTEEEHQKFYDAVTEAYLRVFERVGLGDVTYTTFASGGAFTQFSHEFQTITDA
                     GEDIIYVEKERKLAVNEEVYTDEVLAQLGLDKSKLEKVKAAEVGNIFSFGTAKSEQLG
                     LHFTDEDGTDKAVVLGSYGIGITRLMGVIAEHFADDKGLVWPVSVAPAKVYLARLGDS
                     PEVVKQADELYERLTAASTQVLYDDRDMRPGEKFADADLLGIPYRVVVSEKTVAAGKY
                     ECKARTAEQPEHVTRGELFKKLAVTDIM"
     gene            3543..4001
                     /gene="greA"
                     /locus_tag="VN778_04635"
     CDS             3543..4001
                     /gene="greA"
                     /locus_tag="VN778_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HXR50283.1"
                     /translation="MKKLFRLTQAGVDELADELKTLTAKRAAIAEAIKNARELGDLTE
                     NAEYQSARAEQDRNETRITEIENILQNVEIIKKPRGDNKVQLGSSVKLKNDGKTKEFQ
                     VVGTVEADPLNGKISDESPIGVALLGKKVGDSVDIVTPAETTTYKVVSIS"
     gene            4007..>4364
                     /locus_tag="VN778_04640"
     CDS             4007..>4364
                     /locus_tag="VN778_04640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50284.1"
                     /translation="MPIPEIPQGGFELSTGQLGQLEAAGVPLHDPVDNAAPRNVEEFR
                     QIDINNLGWYFCKWPTDFNHEAAGQYGRYMMPHFQRFYVLYKDFVDETHPIEEELNRL
                     TKENLPYYETPTWDERA"
BASE COUNT         1095 a         1123 c         1150 g          996 t
ORIGIN      
        1 accggaataa actcctcatt cgatacagtc tgaaagggaa tagtcgacag agccgcgaca
       61 ggtgcggctg acgccatctg caaacgcggc cgagctacga ccatgtcatt ccccaccagg
      121 cgggaacgtg taccactcac ttttttaacc tctgtttgtt gtctttttgg cttttaggta
      181 agcttaagaa tcattatata cccggcgcgc agtttgtaaa gcagatttta gcgcggcggt
      241 acgttataat aaggctaatg ggaatacttt tgtttgttat tggcatcttg ctgtttattg
      301 gcctggtcgt ggtgcatgaa tggggtcatt ttattaccgc tcggcgtaat ggcgtcgagg
      361 ccgaggagtt tgggatcttc tttcctccca ggctgtattc gcgtaagacc aaggccggct
      421 gggtcttcag tatcaacgcc ttgccacttg gtggtttcgt caagctgaaa ggcgaacacg
      481 acagcgatac tgaacgaggc agttttggtg ctgcgagcac ctgggccaag agcaaaatca
      541 tggctgccgg agtgatcatg aacttggtga ctgcactggt gctgttgacg atcttggccc
      601 ttatcggcat gccaaaactg atcccaaacc aatataccgt gaaacgcgac acccaactgt
      661 cgagccaaag cgtcattatt ggctacgttg agcctggctc accggcgcaa aaagccggcc
      721 tgcagcccga agacgagctg accgacattg gcctaagggg ctacagcccg gtggcggtca
      781 ataatgccga caaactaccg acggtcacta aagattttgc cggcaagaaa gtgactattt
      841 actacgaacg cgctgggcag caccacgaag ctgaagccac actgcgtacc acaagggtcg
      901 tcgaagctag ccaaaagact aacaatccta taggttatct gggcatttcg ccgacagaat
      961 tcactgtgca gcgttccacc tggtcggcgc cgctcgtggc cgtcggctta agcgcccaag
     1021 tcacaggttt aacgctccaa ggtctcgggc acgcccttgg cggcctaggt agcttaatcg
     1081 ccggcgccgt aacaggcaac acgacggccc ggcagaacgg ccaagcctca gcaacctccc
     1141 aagtcgctgg gccggttggc atattcgtga tcctcaaaga cggcagctta cttggctacc
     1201 agttcatgct gtttattatc gccattattt cgctgacgct ggcgattatg aatattttgc
     1261 caatcccggc acttgatggc gtccggctgt ggctgatgct gctgacgcgc gcgctgaaac
     1321 ggccgttgac gcccaaaaaa gaagaactta tcaatgcggc aggctttgta gtgctcatcg
     1381 gcctcgtgat tttagtgacc attgtcgatg tccaaagatt cttctagccc gactattgcc
     1441 cgcgtccctt ggaatgcctt gggcggcgtc atttttttga ttgtaatctt ctacgtctcg
     1501 caatttttcg caggcatttt gatctctatc tatcccggac tgaaacactg gacggacgcg
     1561 caagcaaaca gctggctcga caattcggta acggcccagt tcatttacat actgctggct
     1621 gaaagcttcg cggtcggtgc gatctacctc tttttgcgcc gttacaaagc taatttttcg
     1681 ttaatcggcc tgaggcggcc gcgctggcgc gatctcggct atggattgat ggccgccccg
     1741 ctttactatg gactgtattt tgtagctgtt ggcgttgtaa cgcactacgt gaaagggctc
     1801 aatgccaatc agcagcaaca gatcggcttc acaaatgtgc atggcagccc gcagctgatc
     1861 atgaccttta taagtctggt ggtattgccg cctattgccg aagaaattat ggtccgcggc
     1921 ttcctctaca gtacgctacg aaaagcgctg cccatgatca gcgccgtact gctgaccagc
     1981 gctatttttg ccagcgccca cttgccggag ggcggggcag ccgggccgct ctggatcgcc
     2041 gcgctcgaca ccttcgtact cagtttattc ctgatctact tgcgcgaggt aacgggcagc
     2101 ttgtgggcca gtattgtcct acatgccacc aaaaacggca tcgcttttgc cgcgcttttt
     2161 gcactccacg tcaaataaca tagtagaata ggtcaggaga aggaacttta ccgatgaaat
     2221 taagccaatt attcactaaa accaccaaga acgtacctgc cgatgaaacg gcacgtaatg
     2281 cccagctttt gatccgggcc ggcttcatcc ataaggaaat ggctggcgta tacgcctatc
     2341 tgccacttgg tttgcgtgta cttgagaaca tcaaacaaat cgtccgtgag gagatgaacg
     2401 gcctcggttc gcaagaattg atcatgacga gtttgcagcg tcaggagctc tgggaaatca
     2461 ccgatcgttg ggacgacaaa caagtcgata tttggtttaa gacgcagctc cagaatggca
     2521 cgcccattgg cttggcttgg tcacatgaag agccaatcac tatgatgatg aagaattata
     2581 ttgccagcta ccgtgatttg ccgacaggcg tgtaccaatt ccagacaaag ctgcggaatg
     2641 agcttagggc caagagcggt atcatgcggg ggcgcgagtt cgttatgaaa gatctttatt
     2701 cttacgcccg gaccgaagaa gagcaccaga aattttacga tgcggtgacc gaagcctatt
     2761 tgcgcgtttt cgagcgagtc ggcttagggg atgtgaccta tacgacgttt gcttccggcg
     2821 gtgccttcac ccagttcagc cacgaattcc aaaccattac cgatgccggt gaagacataa
     2881 tttacgtcga gaaagagcga aaactagccg tcaatgaaga agtgtacacc gatgaggtac
     2941 tggctcaact tggcctcgac aaatctaagc tcgagaaagt caaggcggct gaagtaggca
     3001 acatcttcag cttcggcaca gccaagagtg agcagctagg cctgcacttt accgacgagg
     3061 acggtaccga caaagcagtc gtgctcgggt cgtatggcat tggcatcaca cggctgatgg
     3121 gcgtcattgc cgagcatttt gcggatgaca aaggtctggt ctggccagtg tccgtggcgc
     3181 cggccaaagt gtatctggcc cggcttggcg acagcccaga agtggtcaag caggccgacg
     3241 agttgtacga gcgcttgaca gcggcgagca ctcaggtatt atatgacgac cgagatatgc
     3301 gacccggcga gaagttcgca gatgccgacc tcttagggat accatatcgc gtagtagtaa
     3361 gtgaaaaaac cgtcgcggcc gggaagtacg aatgcaaagc ccggacggct gaacaacccg
     3421 agcacgtaac acgcggcgaa ttgttcaaaa aattagctgt aactgatata atgtagccaa
     3481 acttcgctaa cagatattga ctgcagagac cgcctggata ggtgattaag gagggaatgc
     3541 ccatgaagaa attatttcga ctgacccaag ctggcgttga cgagctggca gatgaactga
     3601 agacgttgac tgccaaacgc gcagccattg ctgaagctat caaaaacgcc cgcgagctcg
     3661 gtgacctcac cgagaatgcc gagtatcaat cagctcgcgc cgagcaggat cgcaacgaaa
     3721 cgcgcatcac cgaaatcgag aatattttgc agaacgtcga gattatcaag aagccgcgcg
     3781 gcgacaacaa agtccagctg ggctcctccg taaagctcaa aaatgacggt aaaaccaaag
     3841 aattccaagt cgtcggcacc gtcgaagccg atcccttgaa cggcaaaatc agcgacgaat
     3901 cacccatcgg cgtcgccctc ctcggcaaaa aagtcggtga tagcgtggat atcgtcacgc
     3961 cagccgaaac caccacttac aaagtcgtca gcatctctta aataacatgc caattcctga
     4021 aattcctcag ggtggatttg aactatcgac agggcagtta ggacagcttg aggctgcagg
     4081 tgtgccgctg cacgatccag ttgacaatgc tgcgccgcgc aacgtcgagg aatttaggca
     4141 gatagatatt aacaatttag gttggtattt ctgtaaatgg cctactgact ttaatcatga
     4201 agcagcaggg cagtatggtc gttacatgat gccccacttc cagcgcttct atgtgttgta
     4261 taaagacttt gttgacgaaa cgcatcctat cgaggaggag cttaatcggt taacgaaaga
     4321 aaatttgccc tactatgaaa cacccacttg ggatgaacgc gcag
//
LOCUS       DATNGP010000179         3069 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_2040621, whole genome shotgun sequence.
ACCESSION   DATNGP010000179 DATNGP010000000
VERSION     DATNGP010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3069
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2040621"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1741
                     /locus_tag="VN778_04645"
     CDS             <1..1741
                     /locus_tag="VN778_04645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50285.1"
                     /translation="GQALQPVQPSRQPVVNPVAPSAPAPDQPEPPAPEAATEAPARPT
                     SRRNIFKRYWYHKLWTLPLTVLAVIGVLMAVPTTRYLALAHFISRPFTVTVTDSTTNT
                     PISGATVKLDGHSAQTDSKGRAAITVPVGDHMLLVSETYYKSTSQAVFVDVSARANSL
                     TLHLLATGRQVPLKVVNKITGQAIPEVEFTIGETTARSDLSGMATVVLPADSPSIPAV
                     IKADGYNTLTTEVQVTTKADPANTFALVPVGRIYFLSNTSGAADLVSTNLDGSGRKTV
                     IAGDPSSSQAAPYLTASHDWRYLALFTVQVNGDSPKLSLVDTSNDKVTTIDTTAAVYT
                     LAGWSGHNFIYTLDRSATVHSWQPNQQTIMTYNADTKQAKAIVNSAATGTSDADAVFQ
                     KIGNVQLFGGNVIYTLTWMKLPDVTAVSGQADTLISAKADGTGSRTLKSVDAGATRID
                     SLTLAAPDKLYFAVIGATSNYYRLDVNGNTTQSSTITSDSVSQSYPVYDTSPSGAATA
                     WMSAKSNILVGDASGNSGSVVNVPAGFVANSAPYGWFSDSYLLLRSSNLGSLYVVAVT
                     GGTPLKIADNISI"
     gene            1807..2619
                     /locus_tag="VN778_04650"
     CDS             1807..2619
                     /locus_tag="VN778_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014455772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HXR50286.1"
                     /translation="MTHEEFLQVLEAYYREHGRHDLPWRSPEPDGTFDPYKILVSEVM
                     LQQTQVARVVPKYAAFLERFPDIASLAAASMGDVLRVWQGLGYNRRAKYLWQTAQQVT
                     SECAGQLPDGAKALEHLPGIGRNTAGALLAYAFNKPALFIETNIRTVYIHHFFRDHID
                     VPDQAILGLLEQTLDRVNPREFYWALMDYGSYLKQTVGNLSRASRGYIKQSQFHGSRR
                     QIRGQILRQLAAGPQTKLTLERQVADERLAPVLESLKREGLICKTGGRYRLP"
     gene            2636..2935
                     /locus_tag="VN778_04655"
     CDS             2636..2935
                     /locus_tag="VN778_04655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50287.1"
                     /translation="MEPNSQPAPVPKPTSQPVMDIKAPARVSASEPVKTPPAEPGMAA
                     PESVAVRKNDDKKAKPPKPSKTHGSSVGLAIVATVLIVLGLGLLFIYAYIQQNGH"
BASE COUNT          718 a          871 c          804 g          676 t
ORIGIN      
        1 aggtcaggcg cttcagccag tgcagccgtc acgtcaacca gtggttaatc ctgtggctcc
       61 ctctgctcct gcaccagatc agccggagcc accagctccg gaagcggcta ctgaagctcc
      121 ggcgcggccg acatccagga gaaatatctt caagcgctat tggtaccaca agttgtggac
      181 cttgccgctg acggttctgg ctgtaattgg tgtgctcatg gctgtgccga cgacgcgcta
      241 tctggcgctt gcccatttca tctcccggcc cttcacggta accgtcaccg acagcactac
      301 caatacacca attagcggcg ccaccgtgaa gctggacggg cattcggctc agacggatag
      361 taaggggaga gcggcgatta cagttccggt tggcgaccat atgctcctag tcagcgaaac
      421 gtactacaaa tcgacttcgc aagcggtgtt cgttgatgtc tccgcacgag ctaactcgct
      481 aacgctgcac ctcttggcaa ccggccgcca ggtaccgctg aaggtggtca acaaaattac
      541 gggtcaggcg attcccgagg ttgaatttac catcggtgaa acgacggccc ggagtgatct
      601 aagtggtatg gcgacggttg tcctgccggc agatagcccg agcatccctg cagttatcaa
      661 ggcagacggc tataacacgc taacaaccga ggttcaagtt acaactaagg ccgaccccgc
      721 caacacattc gctctggtgc cagttggccg tatttatttc ctatccaaca ccagcggtgc
      781 agctgacctt gtatcgacta acctggatgg atccggtcgc aaaacagtca tcgccggtga
      841 tccaagctcg agccaagcag ctccttactt aactgcttcg cacgactgga gatacttagc
      901 gctatttact gtgcaagtaa acggtgattc gccgaaactg tccctggttg ataccagtaa
      961 cgacaaggtt acgaccatcg acacaactgc tgctgtgtac acgctcgctg gttggagtgg
     1021 ccacaacttt atctatacac tcgaccggag cgctaccgta cactcttggc agcctaacca
     1081 gcagactatc atgacttata acgctgatac caaacaggcc aaagcgatcg ttaactctgc
     1141 ggctaccggt accagtgatg ccgacgcggt atttcaaaag attgggaatg tccaactgtt
     1201 cggcggcaac gttatttata cgcttacctg gatgaagctg cccgatgtaa ctgccgtcag
     1261 tggacaggcc gatacgttga tcagcgctaa agctgatggc accggcagcc gaacgctcaa
     1321 gagcgtcgat gctggtgcga cccgtattga cagcctgacg ctcgccgcgc ccgataagct
     1381 ctactttgcg gttatcggtg caacatccaa ctattaccgg ctggacgtca atggcaatac
     1441 cactcagagt agtaccatta cgagtgatag tgtttcgcaa agctatccag tgtacgacac
     1501 ctccccctct ggggcggcga cggcgtggat gtcggccaaa agcaacattc tcgtcggaga
     1561 cgcgagcggc aatagcggta gcgtggtcaa cgttccagcc ggctttgttg ccaactccgc
     1621 gccatatggc tggtttagcg acagctatct gctgcttcgt agctctaatc tcggcagcct
     1681 gtacgtcgta gccgtaaccg gtggcacgcc gctcaaaatt gccgataata tctcaatata
     1741 gttgcggtat ccggggcgga aaacgcttgc ctcagccttg tccgcagggc acaatgagct
     1801 gttgtgatga cccacgaaga gtttttgcag gtactggaag catactaccg cgagcatggc
     1861 cgtcatgatt taccgtggcg ctcgcccgag cctgatggca cgtttgatcc gtataagatt
     1921 ctcgtttcgg aagttatgct gcagcaaacg caagtggcgc gggtagttcc caagtacgcg
     1981 gcatttctag agagatttcc ggatatagca tcgcttgcag ccgcatccat gggtgacgtt
     2041 ctgagggtct ggcaagggct tggctataat cgtcgcgcca aatatttgtg gcaaaccgca
     2101 caacaggtaa cgagcgaatg tgctggccag ttgccagacg gagctaaggc gctcgagcat
     2161 ttaccgggaa ttggtcgcaa tactgccggg gcactgctcg cgtatgcatt caataagcca
     2221 gcactattca tcgagaccaa tatccgcacg gtatatatcc accatttttt ccgcgaccac
     2281 atagatgttc ccgatcaggc aatcttggga ttgctcgagc aaacgctcga ccgcgtcaac
     2341 ccccgcgaat tttactgggc gctcatggat tatggctcct acctaaagca aaccgtaggc
     2401 aatctctcgc gtgccagcag aggctatata aaacaatcac agtttcatgg ctcgcggcgt
     2461 caaattcgcg gccagatttt acggcaactc gctgccggcc cgcaaacaaa actcacgctg
     2521 gagaggcagg ttgccgatga gcgactcgcc ccagtgctcg agagcctgaa acgtgaaggc
     2581 ctaatctgta aaacaggtgg ccgctaccgg ttgccatgat acaatgctta tgactatgga
     2641 gccgaattcc cagcccgcgc cagtcccgaa accaacctca cagccggtaa tggacatcaa
     2701 agctccggcc cgcgtatcag catccgagcc tgtcaaaact ccgcctgccg agcccggaat
     2761 ggcagctccc gaaagtgttg cagtcaggaa gaacgacgat aaaaaagcta agccgccaaa
     2821 accgtccaaa acccatggta gtagtgttgg tctggcgatt gtcgccaccg tacttattgt
     2881 gttgggatta ggcctgctct tcatctacgc ctacatccaa cagaacggac actagccagt
     2941 tagcctaggc atatatggat aagcggtatt tgtaccatct gtggactagg gtccggcccg
     3001 ttcgaccctg gtatttcctg gtgttgactg cgttacttct tggcctggca attgtggcac
     3061 tgcgcgcca
//
LOCUS       DATNGP010000180         3903 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_3581290, whole genome shotgun sequence.
ACCESSION   DATNGP010000180 DATNGP010000000
VERSION     DATNGP010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3903
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3581290"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(42..848)
                     /locus_tag="VN778_04660"
     CDS             complement(42..848)
                     /locus_tag="VN778_04660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50288.1"
                     /translation="MDKQEFGIRTALIKQQLGNDELFQDIEYEARQDERVRNMAERIK
                     GLRVANRAAEADELVSANRDLIDDARREAMIERGLQGDINEMEQKLEERRLLQLEKIH
                     EGLTGEESDSELLKAFHLTDEEGRFSDDTFHSPLFRERTKMAFEAYRTAVTSFEALVD
                     YNRLYGVPSKNVGPADSLRGDAHNEVARQVAEDLGLEFKTARSLVAKIRDGKIPRSGE
                     EETYKRSIFRIGKRLSRRFGDDLAEAVKEDLKPIFGSEESDEDSHNHHLH"
     gene            complement(932..2230)
                     /locus_tag="VN778_04665"
     CDS             complement(932..2230)
                     /locus_tag="VN778_04665"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008765 -
                     UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HXR50289.1"
                     /translation="MNFRKIIKRAIPRGLFPAIEPYGHLGEAFLFNVLNGFPGRGLKV
                     IGVTGTNGKTSTTFLIHRMLHEAGYKVGIMSTVGFGIGADINPQIHHMTNVSVPELME
                     RLKWMKKQGVEWLVLETTSHALAQNRVWGIPYSVAVMTNVTHEHLDYHRTFERYRDAK
                     RKLFKLANHNKKGMRLGIINAEDPSAELFASDIKNPVLYGVEQGDVQAHEVKLAPSGV
                     KYRVDFEGKRYDITCQLPGSFNVYNSLAAVSVGIKLGLTPEQIEKGIAALEGVEGRMT
                     RIDEGQDFDVIVDFAHTPDSFEKLFKDLKPVVKGKLIVLFGSAGRRDEAKRAVQGELA
                     GKYCDEVVVTEEDDRDVDGLEIMNQIAEGAEKAGKVRDLDLFLVHDRPEAINFAVRRA
                     EKGDTVLLLGKGHEKTIERADGEHPWDEIGTTREAVRLTK"
     gene            2255..3310
                     /gene="tsaD"
                     /locus_tag="VN778_04670"
     CDS             2255..3310
                     /gene="tsaD"
                     /locus_tag="VN778_04670"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HXR50290.1"
                     /translation="MKILGIETSCDETAAAVVEDGKRLLSSVVASSMDLHVPYGGVVP
                     EIAARSHIEVILPVIHQALDEAACTWDDIDAIAVTYGAGLGGSLLVGVMTARTLAIAL
                     NKPLYAINHVEGHVYANFLTETVLPGYTLRAAPPQFPMLAIIVSGGHSQLALFRDHFD
                     YTLLGQTQDDAIGEAFDKVAKMLGLPYPGGPSVEKAAQAGKPTYTLPKAKMPGYDFSF
                     SGIKTAVLRLAQAEIGESYDFPSTKLPERLSEAQKANIAASFQRVAIETIVDKANRAC
                     EEFQPKSVVIAGGVAASAELRRQLTEALLLPIEFPDLKLCTDNGAMIATLGGFKAMRN
                     QPAADPYSLAVEPDLSM"
     gene            complement(3303..>3903)
                     /locus_tag="VN778_04675"
     CDS             complement(3303..>3903)
                     /locus_tag="VN778_04675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50291.1"
                     /translation="ENFNFLVISPCKPNVQGDRHLSVVGFGVSGWQKGTDAVDEVMEP
                     ITEYDDLAALGDPSLTSIDRIGKGIGPRLYNTPQALVGTEENPRRIGAYKLWLDVASV
                     WDFRLTLDPETPAGQGIRIAKQAYFRAYARLRGERKAAQHIHSGHLSRQAFMHHQTPT
                     GLRLEKQRRAAPSAIPEAFLVIRNPNLRAYKIGEYTRPT"
BASE COUNT          846 a         1104 c          955 g          998 t
ORIGIN      
        1 atggagcaca ataagctcta aatactgctt aagggtttag ttcagtgcaa atgatggtta
       61 tgagagtctt catcagactc ttctgaaccg aatatcggct tcagatcttc cttaactgct
      121 tcggcaagat catcaccaaa acgtcgagat aatcttttac cgatacgaaa aattgatcgc
      181 ttgtaggttt cttcttcgcc tgagcgcggg attttgccat cacgaatttt agctacaagg
      241 ctacgagcag ttttaaattc cagcccaaga tcttctgcta cttgtcgagc gacttcattg
      301 tgagcgtcgc ctctcagtga gtcagcgggg ccaacgttct tacttgggac gccgtagagt
      361 ctattgtagt ctaccaatgc ttcgaatgag gtaaccgctg tccggtaggc ttcgaaggcc
      421 attttggtgc gctctcggaa aagaggagaa tgaaacgtat cgtcactaaa ccgaccttct
      481 tcgtcagtta aatggaaggc ttttagtagc tcactgtcag attcctcacc tgtaagaccc
      541 tcgtgaattt tttctagctg taacaagcgt cgctcttcaa gcttttgctc catctcgtta
      601 atgtcaccct gtaagccccg ctcaatcatg gcttcgcggc gtgcatcatc gatgaggtct
      661 cggttggcac taaccagttc atctgcttcc gcagcccggt tggcaactct cagcccctta
      721 attcgttccg ccatattccg cacccgttcg tcttgtcgcg cctcatactc aatatcctgg
      781 aacaactcat cattgcccaa ttgctgttta atcaacgcgg tgcgaattcc aaattcttgc
      841 ttatccatag ttgtgaggct ttttgcaggt aatagtttta atgttagctg aaattattaa
      901 ttaattatgc tataaattat acacattttt ctcattttgt caatctgact gcttccctag
      961 ttgtgccgat ctcgtcccaa ggatgttcgc catcggcgcg ttcgatggtt ttttcgtggc
     1021 ctttgcccag gagcaggacg gtatcgccct tctctgctcg cctcacagcg aaattgatgg
     1081 cttcggggcg atcgtgcacc aggaagaggt cgaggtcgcg gactttgccg gctttttcgg
     1141 cgccttctgc aatctgattc atgatttcta agccgtcgac gtcccggtca tcctcttcgg
     1201 tcactaccac ttcgtcgcag tacttgccgg ccagctcgcc ctgcacggcc cgcttggctt
     1261 catcgcggcg gccagcggag ccaaatagta cgatcagttt gcctttgacg actggcttca
     1321 agtctttgaa taatttttcg aagctgtcgg gtgtgtgcgc aaaatcgacg atcacgtcga
     1381 agtcttgacc ctcatcgatc ctggtcatgc gtccttcgac gccttccagc gcggcgatgc
     1441 ctttctcaat ctgttctggg gtcaggccta gcttaatgcc gacgctgacc gccgctagtg
     1501 aattatagac attgaaactg cctggcagct ggcatgtgat gtcgtagcgc ttaccttcaa
     1561 aatccactcg atactttaca ccagacggag caagtttgac ctcgtgggcc tgcacatcgc
     1621 cctgctctac cccatataaa actgggtttt taatatcgct cgcaaacagt tcggcactcg
     1681 ggtcttcggc atttatgatc ccgagtcgca tacccttttt attgtgatta gccagcttga
     1741 agagtttgcg cttggcgtcc cgatagcgct caaacgtccg gtggtaatcc aagtgctcgt
     1801 gcgtcacatt ggtcatgacc gccaccgaat acgggatgcc ccaaacgcgg ttttgcgcca
     1861 gagcgtgaga agttgtttcg agcaccagcc actctacccc ctgttttttc atccatttca
     1921 gccgctccat cagctctggc acgctgacgt tggtcatgtg gtggatttgc ggattaatgt
     1981 ccgcgccaat gccaaagccg acggtgctca taatgccgac cttgtaaccg gcttcgtgga
     2041 gcatccgatg gatcaagaat gtcgtcgacg tcttaccgtt cgtccctgtc acgccgataa
     2101 ctttgagccc gcggccaggg aaaccattca gtacattgaa gagaaacgcc tcccccaaat
     2161 gcccgtacgg ctcgatggcc gggaacaggc cgcgcgggat cgctcgctta atgattttcc
     2221 gaaaattcat cactctcagt atactgtaag tctagtgaaa atattgggca ttgaaaccag
     2281 ctgcgacgaa acggcggcgg cagttgttga agacggcaag cggctgctga gcagcgtcgt
     2341 ggcctcgagc atggatttgc acgtgccgta cggcggtgtc gtgccggaaa ttgccgctcg
     2401 cagccatatc gaagttatct tgcccgtcat tcaccaggcg ctcgatgaag ctgcttgcac
     2461 ctgggatgac atcgatgcta tcgccgtaac gtacggggcg ggcttaggcg gcagcctcct
     2521 cgtcggtgtc atgacagctc gtactcttgc catagctctc aacaaacctc tctacgctat
     2581 caatcacgtc gaggggcacg tctatgctaa cttcttaacg gagactgttc tgcccggtta
     2641 caccttgcgg gctgcaccgc cccaatttcc gatgttggca attatagttt ctggcggtca
     2701 tagccaactg gccctctttc gagatcactt cgactacacc ttgctcggac aaactcaaga
     2761 tgatgccatc ggtgaagcct ttgacaaagt cgccaaaatg ctgggcctac cctaccctgg
     2821 tggcccttcg gtagaaaaag ctgcccaagc cgggaaacct acctatacat tgcccaaagc
     2881 taagatgccc ggctacgatt ttagctttag cggcataaaa acagccgttc tacggctagc
     2941 acaagcggaa atcggcgagt catacgattt cccctctaca aagctgccag agcgcctctc
     3001 tgaggctcag aaggccaaca ttgccgccag cttccagcgc gtcgccatcg aaaccatcgt
     3061 cgacaaggcc aaccgggcct gcgaagagtt ccagccgaaa agcgtcgtta tcgccggcgg
     3121 cgtcgcggcc agcgccgaat tgcgtcgcca gttaacagag gcacttctct tgcccatcga
     3181 gttccccgat cttaagctct gcaccgacaa cggcgccatg atcgccaccc tcggcggctt
     3241 caaggccatg cgcaaccaac cggcggctga cccctatagc ttagctgtcg aaccggacct
     3301 atctatgtag gccgcgtgta ttcaccgatt ttgtaggcgc gcaggttcgg gttgcgtatg
     3361 accaaaaagg cttccggtat ggctgacggc gcggcgcggc gctgcttctc taaacgcaag
     3421 cccgtaggtg tttgatggtg cataaaagcc tgacggctca ggtgaccgga gtgaatgtgc
     3481 tgcgccgctt ttctctcccc tctaagacgg gcataggccc ggaaatatgc ctgcttggca
     3541 atccgtatac cttgtccggc cggagtttcc ggatctaacg ttaggcgaaa atcccataca
     3601 cttgcaacat caagccagag tttataggca ccaatccgac gagggttttc ttcggtgccg
     3661 accagcgcct gcggcgtatt gtacagtcgc ggcccaatcc ccttgcctat tcggtcaata
     3721 ctggtgagtg atggatctcc gagggcggcc aaatcgtcat actcagttat tggctccatt
     3781 acctcatcaa ctgcatcggt gcccttctgc cagccagata ccccgaagcc aactacagat
     3841 agatggcgat ctccctgcac attaggctta cacggggaga tgactaaaaa gttaaaattt
     3901 tcg
//
LOCUS       DATNGP010000181        10803 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_1180278, whole genome shotgun sequence.
ACCESSION   DATNGP010000181 DATNGP010000000
VERSION     DATNGP010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 10803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10803
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1180278"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1348)
                     /locus_tag="VN778_04680"
     CDS             complement(<1..1348)
                     /locus_tag="VN778_04680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50292.1"
                     /translation="MVGFQTKILKVLASPQFFAAILLLFFIESAWIAISAAYPMVFDE
                     NAHFAVIQAYAHHLSPFFPAHSTLMDSSGALSRNPSFLYRWLMSFPYRLISAVTGDQM
                     MQVIDLRFINIFLFGGSLVLFRRLLLKTRVSPAIVHATLLFFVLTPVVPLLAGQINYD
                     NLIMLVTAMALLMATTISEGLRRERLLPIGRCLWLLTLCLLGSLVQFEFLPVFAAIVL
                     WLSWQAWRTRRTQTIGPGTQLRQAWHKNSWTQKLLITVPLVLALGIFIEMYGVNLVRY
                     HNLTPQCGQVLSAQQCATNVTWVRSQEALAHKATVNTNPGLFAVSWTYRMFIAMFYTS
                     SGGAGPQANYLSINPLPLIFITALAVFGAGTVLFIKYRGALFDGYEYLGFLLFVSALY
                     VGALWLRNYIDYVHLGIKIAINGRYLFPIALPCMIIIALAFRQLFGHRTHLKAALLM"
     gene            1498..1740
                     /locus_tag="VN778_04685"
     CDS             1498..1740
                     /locus_tag="VN778_04685"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HXR50293.1"
                     /translation="MGTKTISASELRSNLADALEAVNQDEILIITRRGKKERAIIDLD
                     KLEDLLASADPAYLKKIKEARESQEYFSHEEVFGDL"
     gene            1774..2010
                     /locus_tag="VN778_04690"
     CDS             1774..2010
                     /locus_tag="VN778_04690"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HXR50294.1"
                     /translation="MAVKEIRKLSPQIQKRLGIKLQWFAGQEDPLKFAEPLTKPADAG
                     YRFRIGNYRVLFDVEDDTIVILYVQHRREVYKNK"
     gene            complement(2014..3108)
                     /locus_tag="VN778_04695"
     CDS             complement(2014..3108)
                     /locus_tag="VN778_04695"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HXR50295.1"
                     /translation="MSKIVIDARESGTSTGRYIDKLIEYLHKLQPAHKIVVLTGEHRV
                     DFYKQVTPSFEVVATPFKEFSYGEQFGFKKQLEGLQPDLVHFGMAQQPVRYHGRSVTT
                     IHDLTTMRFTNPDKNLLVFKFKQQVYKYVVKQAARKSVAILTPSQFVKDDVVRFTGVN
                     PDRITVTYEAADAITEAAEPIESLQGKQFLMYVGRPTPHKNLERLIDAFQLLKGDNPG
                     LHLVLSGRQDMNYRRIEAKVSKRHLKDIIFTGYTSEGQLRWLYESCAAYVFPSLSEGF
                     GLPGLEAMVHGAPVVSSNATCLPEVYGDAAHYFDPLDVQAMAGAINKVLSDKNLRNEL
                     VQTGKLQATQYSWRRMAEQTLAAYEQALRV"
     gene            complement(3105..4115)
                     /locus_tag="VN778_04700"
     CDS             complement(3105..4115)
                     /locus_tag="VN778_04700"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HXR50296.1"
                     /translation="MSKACVVIPNWNGADSLAACLDSLLKQSEKPHMIVVDNGSTDGS
                     LELLEKYPEVEVIKHVENRGYAGGMNPGLQRAIDSGAEYVAAFNNDAVADKRWLRELV
                     QALDKQPEAGIATCKLLSADGKHIDSTGDYFTVWGLPYPRGRGETDLTKYDTQTEIFG
                     ASGGASLYRVSLLQEVGLFDEDFFAYYEDVDLSFRTQLAGWKVVYVPSAVAYHQISAT
                     SNRLKGFATYQTMKNQPLLVYKNVPRRYLFRVGWRFTLAHVLFYLRAFTRGQGWMATK
                     GDLKGTNLLFKKAKERRRIQRTKKVSDEYIWSIMVHDLPPNARNLRKLRDAWWKLLRK
                     RV"
     gene            4156..4956
                     /locus_tag="VN778_04705"
     CDS             4156..4956
                     /locus_tag="VN778_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003815140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXR50297.1"
                     /translation="MRHIFSKQNRALLSELVRTDFKLRYQGSVLGYAWSLLRPLFMFA
                     ILYVVFTRVFSLGKSVPHYPVYLLAGIVIWNFFLEMTMQSTTSIVGRGDLIRKIRIPR
                     WMIVFSTSLSALINLGLNLIIVAIFMVINHVGILGTLPLVILPLLEVYLLALGLSFLL
                     AALFVKFRDVSYIWEVLMQAGFYATPIIYPLSRIANISFQKLIMLNPMAQAIQDVRYT
                     AISHNTITAYKLFQGGWHALVPFIFVAAAVVIGPIYFRSQSKYFAENI"
     gene            4960..6162
                     /locus_tag="VN778_04710"
     CDS             4960..6162
                     /locus_tag="VN778_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008578618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXR50298.1"
                     /translation="MADNIAINVDKVSKYFKLPHEKNSSIKGAVVNFWRRDRTFEKQQ
                     VLNDVSFSIKNGEFFGVVGRNGSGKSTLLKLLAGIYNPSKGSLQVNGKLTPFIELGVG
                     FNPELTGRENVFLNGALLGFSRANMAAMYDDIVDFAELHRFMDQKLKNYSSGMQVRLA
                     FSIAIRAQSDILLLDEVLAVGDAAFQKKCYDYFMELKRQGRTVILVTHDMGAIRQYCT
                     RAMMIDNGKIVKEGDVEKVAQAYLALFSEENKTKQSKSEDDDRWGNGLQTTTRAKVKT
                     TDEEIIITTEFTAHAHIESVMYGFTLYEKASKTNIFESNTKRLRLKMPPVVENEITTL
                     EWRAPNILRNGEYAISSSTCDESTLTYYEWLNGADTFKISNKVETAGAVNPVVKLKVT
                     SKKDDIKG"
     gene            6244..8100
                     /locus_tag="VN778_04715"
     CDS             6244..8100
                     /locus_tag="VN778_04715"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HXR50299.1"
                     /translation="MKGFLLATVGAQHWGRFKYRQWLDRNFPDFIEIAKLRKIDQSLK
                     YRPLISVVVPTYSPKPQFLRDCIESVQGQIYENWELCIVDDASPDETTREIIKEYSAN
                     DDRISFKFLETNQHIAQATNEAIKMAKGDFVALFDHDDLLWPNALLEVVRALNNNQKL
                     DFIYSDEDKITEDRFVHPGAFFKPDWSPDFLHSVNYITHLSVVRKSLLDKLGGLNGEY
                     NGAQDWDLFLRITSTTKNIYHIPKVLYSWRVHDLSTAKTTKSKPYVIEAQRKAITADL
                     KRRGYENATVEQDPKHQGYWHVEQPIKGNPLISIVIPTKNQYKVVKRCVNSIFKKTTY
                     NNFEIVLVDTGSTDKKVRSWYKKLQQKHTNVRIINWPEQPFSYSRSCNKGAQAAKGEL
                     LVMLNNDTEVITPNWLELLAGDAQRPEIGAVGCLLFFPDGRHIQHAGIGVGLGGVAAN
                     SFSMMTLMQPMTQTQHLMINTKHNMSAVTAACMMIRTSVFEEVGEFAEEFRVTYNDVD
                     LCLRLREKGYEILYTPYVRLIHHESISVGMPEEIKKRDTSEFREAKELFVTRWANYVQ
                     HDPHLNPNLSKDDAFYDVPDIADLSNDSKMSQEDKPAIERRLRRRKLFVNSP"
     gene            8164..9627
                     /locus_tag="VN778_04720"
     CDS             8164..9627
                     /locus_tag="VN778_04720"
                     /inference="COORDINATES: protein motif:HMM:NF021436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2142 domain-containing protein"
                     /protein_id="HXR50300.1"
                     /translation="MPKATNRAPAKTTKWWHNPSLIFLMLGAIFGITFVTINPPGFGL
                     DERAHFLRAYELSQGHFLTTRTGVDQHIGARIPANIGQWVHDSTINLLSVNTSASLSN
                     RHDADTKLYTKYYHQRLSKETVDDFATNQQLAGTFNYSPLSYLPLAAPVWAGIKLHMN
                     FMSILYLARITNLLLYLLVVYIAIRIAPRFKWLIFIVALLPTAVFQASSVSIDPIINS
                     LSFLLLALFLLIWQQAPKPISLRQAFITLSILGFLAISKQPYALFGLMFLALPRAAFA
                     NKKSEIAYKLTWASTPLLLAIIWSVLDRNTIKQLSTEYLQANVSTVQQIKFIIHGPLS
                     FAAVIARTYYEKSGAFLATMIGAIGDRSVGLSNLILVPAAALLMGLGVIHPSSDIAPP
                     RRKRWTAAWLLVVALGVIGAITAALYIGASNVAARVVYGIQGRYFIPVLPFIGVAVSI
                     LLRERVRVSRALIENTMIAGSSLLLLGTTIFYYIVNY"
     gene            complement(9659..>10803)
                     /locus_tag="VN778_04725"
     CDS             complement(9659..>10803)
                     /locus_tag="VN778_04725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50301.1"
                     /translation="AWGTGSVNYQSGVTTTSRGSSSGTLNVRLNGTCCPGGRCSYGSG
                     SGYKGLVQFWNDPGNYIAFGLIHDPGVSPTGTTLMIEGAAGGRPVGGYWGNNGISGTS
                     HLFTFNWTAGGISVTIDNNVTLGPYPVAENNPSISFLAAGRNTGDIADTTFTNISFST
                     GSITAEPVTIPAGSPYLTYTATMNEAGSGTGYSSYINAHDGSNNAISVGIQTDSSQPE
                     SHGQPYYIWELVQNGTFTYDYLGPAASNTNEPVTLKWWSGDNTAVFYEGNTAVADIDA
                     TLVPRLFFNVEGNARVNGDSVNDSFTNTQISAGDTCPTYCGLNGSWNTSSFNFYGLTA
                     TNTNGQPQNGANFTVAGTVSGLPAGGTWDTNEVAGIGMIAQYWNGQ"
BASE COUNT         2917 a         2584 c         2618 g         2684 t
ORIGIN      
        1 ccatgagcag cgctgctttc agatgagtgc gatgtccgaa tagctgccgg aaagccaggg
       61 cgataatgat catgcagggc agggcgatcg ggaacaggta gcggccattg atcgcaattt
      121 tgatgcctaa gtgaacgtag tcgatgtagt ttctcagcca cagtgcaccg acgtacagtg
      181 ccgagacaaa gagcagaaag cccaaatatt cataaccatc gaacagcgcg ccccggtact
      241 tgataaacaa cacggtcccg gcaccgaaga cggctaaggc ggtgataaaa atcaggggca
      301 gggggttgat gctgagatag ttagcctggg gcccggcgcc gccactcgag gtgtaaaaca
      361 tagcaataaa catccggtac gtccagctga ccgcgaacaa gcccgggttg gtgttgacgg
      421 tggctttgtg agccagggct tcttggctgc gcacccaggt gacattggtg gcgcattgct
      481 gagcgctcag gacttgcccg cactgcggcg tcaggttgtg gtagcgcacg aggttgacgc
      541 cgtacatttc tatgaaaata cccaaggcta acaccagcgg gacagtgata aggagttttt
      601 gcgtccatga atttttatgc caggcttgcc gcaattgcgt ccccggaccg atagtttggg
      661 ttcgccgtgt ccgccaggcc tgccaactaa gccacagcac gatggcggca aaaaccggca
      721 agaactcgaa ctgcactaaa ctgcctagca agcacagcgt cagtagccac aaacagcggc
      781 cgatgggcaa gagacgctca cgccgcaaac cctcactaat agtggtggcc atgaggagcg
      841 ccatcgcagt tacgagcatg atcaagttgt cgtaattgat ttgaccggct aataatggca
      901 cgactggcgt caatacgaag aagagcagcg tggcgtgcac aattgccggt gacacgcggg
      961 ttttgagcag taggcgccgg aaaagcacta gcgaaccacc gaacagaaag atattgataa
     1021 acctgaggtc aattacttgc atcatttgat caccggtgac ggcggaaatc agccgataag
     1081 ggaaactcat cagccagcgg tagaggaagg aggggttgcg cgacaaggct ccggaggaat
     1141 ccatcagcgt ggaatgggca gggaaaaagg gactcaggtg atgcgcatag gcctgaatga
     1201 cggcaaaatg cgcgttctca tcaaacacca tcggataggc tgccgagata gcgatccagg
     1261 ccgattcaat aaaaaatagc aacaagatgg ccgcaaaaaa ctgcggtgaa gccagaacct
     1321 tcaggatttt tgtttgaaat ccaaccatta ccgctatcta ttataacctt ttaccgcaag
     1381 cagcatacag gatgaagctg aggggtcgct gggcgactac atgataacag gttgacgcgc
     1441 aagaaaatgt aacgtacaat ccagatgtaa tatttaatcg tacaattgag gacaactatg
     1501 ggcactaaaa caatttcagc ctccgagctc agaagtaatc ttgccgatgc tctggaagcg
     1561 gttaaccagg acgaaatact tatcataacc cggcgtggca agaaagaacg ggcgataatt
     1621 gatctggata aacttgagga tctactggct tcggccgatc cagcttacct taaaaagatt
     1681 aaagaggccc gcgaaagtca ggagtatttc agccacgaag aagtgttcgg agatttataa
     1741 acagtggcta catacaagct gctttataag agggtggcag tcaaagaaat ccggaagctc
     1801 tcgccgcaaa tccaaaaaag actaggcatt aagctgcaat ggttcgccgg ccaagaagat
     1861 cccctaaaat tcgcagagcc gttgacgaaa ccagctgatg ccgggtatcg ttttcggatc
     1921 gggaactatc gggtactttt tgatgtcgaa gatgacacca tcgttatcct ctatgtgcag
     1981 catcgtcgag aggtttataa aaataaataa tgcctaaacc cgtagcgctt gctcataagc
     2041 agctagtgtt tgttcggcca tgcggcgcca tgaatattgc gtggcttgca gcttgcccgt
     2101 ttggaccagc tcatttcgta gatttttatc ggacaacacc ttgttaatgg cgccagccat
     2161 ggcctgcacg tcaagcggat caaaatagtg cgcggcatcg ccgtacacct caggcaaaca
     2221 ggttgcattg ctgctgacta ccggggcgcc gtgcaccatt gcctccagcc ccggtaggcc
     2281 gaagccctca ctcaagctag ggaaaacgta ggcggcgcag ctctcgtata accagcggag
     2341 ttggccttcg ctggtatagc cagtgaaaat gatgtctttg aggtgccgct tagacacctt
     2401 cgcttcgata cgccggtagt tcatatcctg tctgccgcta agcaccaggt gcaaaccggg
     2461 attatctccc ttcaacaact gaaacgcatc aatcagccgc tccagattct tgtgtggtgt
     2521 cggccggccc acatacatca aaaactgctt accctgcagg ctttcgattg gttcggcagc
     2581 ctcagttatg gcgtcggctg cttcataggt aacggtgatc ctatcaggat taacaccagt
     2641 aaagcgcacg acatcgtctt tgacgaactg agaaggcgtc agaatggcta ccgacttgcg
     2701 ggccgcctgc ttcacaacgt atttatatac ttgctgctta aacttgaaca ccagcaggtt
     2761 tttgtccgga ttggtaaagc gcatcgtcgt caggtcgtga atagtcgtca cgctccgtcc
     2821 atggtagcgc accggctgct gtgccatacc aaaatgcact agatcgggct gtaggccctc
     2881 aagctgtttt ttgaagccaa actgctcgcc gtaactaaac tctttaaatg gcgtcgcgac
     2941 tacttcgaac gagggcgtca cttgcttata gaaatctaca cggtgctcac cagtcaacac
     3001 tacgatcttg tgagctggct ggagtttatg caagtactcg attagcttgt ctatgtagcg
     3061 cccggtgctg gtaccggact ctcgggcatc gataacgatc ttgctcatac ccttttcctt
     3121 aggagcttcc accaagcgtc gcgcagctta cgcagatttc gggcgtttgg cggcaggtca
     3181 tgcaccatga tgctccagat gtattcatcg gaaacttttt tggtgcgttg aatgcgacgc
     3241 ctttctttag cttttttgaa aagcaggttc gtgcctttta ggtcaccctt ggtcgccatc
     3301 cagccctgac cgcgcgtaaa agctcgcaga taaaacaaga cgtgggccag cgtgaagcgc
     3361 cagccgaccc gaaacagata gcggcgcggc acattcttgt agaccagtaa gggttgattc
     3421 ttcatcgtct gataggtggc aaaacctttc aggcgattgc tggtggcgct gatttggtgg
     3481 taggcaacag cactcggcac ataaacaact ttccagccgg ccagctgtgt ccgaaagctg
     3541 aggtcgacat cttcgtagta ggcgaagaaa tcttcgtcaa acaagccgac ttcctggagt
     3601 aaactgaccc gatacaggct agcgccgcca ctggcgccaa aaatctcggt ttgagtgtcg
     3661 tacttggtga gatcggtttc gccccggccg cgcggatatg gcaagcccca caccgtaaag
     3721 tagtcgcccg tgctatcgat atgcttacca tcggcgctga gcagtttgca ggtggcgatg
     3781 ccagcctccg gttgtttgtc gagggcctgg acgagctcgc gcaaccagcg cttatcggcg
     3841 actgcgtcat tgttaaaggc cgccacgtat tccgccccgc tgtcgatggc tcgttgcagg
     3901 cctgggttca tgccgccggc atagccacgg ttttcgacgt gtttgataac ctcgacctcg
     3961 gggtattttt cgagcagctc caatgagccg tcggtggagc cattgtcaac aacaatcata
     4021 tgtggttttt cagactgctt gagcagcgaa tccaggcacg cggcgaggct atccgcaccg
     4081 ttccagttcg gtatcaccac gcaggcctta gacatcgact aactcaagca tttcctctat
     4141 aataacgcat aacgaatgag acacattttc tcaaagcaga accgcgcttt gctgtccgag
     4201 ctggtgcgca ccgatttcaa acttcgctac cagggttccg ttctcggcta cgcttggtcg
     4261 ctgctaagac cgctcttcat gtttgcaatt ctgtatgtag tttttacgag agtgttttcg
     4321 cttgggaaaa gcgttccgca ttatcctgtc tatctgctcg ctggtatcgt tatctggaat
     4381 ttttttttag aaatgaccat gcaaagcacg acatccattg tggggcgcgg agacctgata
     4441 cgtaaaatcc gcatcccccg ctggatgatt gttttctcta ccagcctgtc ggcacttatc
     4501 aacttgggct tgaacctcat catcgtggcc atattcatgg tcattaacca tgtcggcatc
     4561 cttggcactt tgccacttgt catactgccc ttactagaag tttacctatt ggcactcggc
     4621 ctttcttttc ttttggccgc cttgttcgta aaattcagag acgttagtta tatttgggaa
     4681 gttttgatgc aagcgggctt ttacgccacg ccaatcatct accctttgtc gcgcatagcc
     4741 aatatttctt tccaaaaact tattatgcta aatcctatgg cgcaggctat ccaggatgtg
     4801 cgttacaccg ctatttcaca caataccata acggcataca aactattcca aggtggttgg
     4861 cacgctctcg taccatttat ttttgttgca gctgccgtgg ttataggtcc tatttatttc
     4921 cgaagtcagt ctaaatattt cgcggagaat atctaagtta tggcagacaa tattgcgatt
     4981 aacgttgata aagttagtaa gtacttcaag ctgccgcacg agaagaattc atcaataaag
     5041 ggcgcagttg ttaatttttg gcggcgtgat cgaacctttg aaaagcagca agtgctgaac
     5101 gacgtatcgt tttcaataaa gaatggagaa ttcttcggag tagttggtcg caatgggtct
     5161 ggcaagagta cgttactcaa gcttttggcc ggcatttata acccaagtaa aggcagcctt
     5221 caagtaaatg gcaagctaac gccgtttatt gagcttggcg ttggctttaa cccagaatta
     5281 acggggcggg aaaatgtgtt tttgaatgga gcgctcctag gattttcgcg agctaacatg
     5341 gccgcaatgt acgatgacat cgttgacttc gcagagctgc atcgcttcat ggaccagaag
     5401 ttaaaaaact attcctcagg catgcaagtt cgacttgcgt ttagtattgc cattcgggct
     5461 cagagcgaca tcctgctatt agacgaggtc ttagctgtgg gcgacgcggc ttttcagaaa
     5521 aaatgctacg actattttat ggagctaaaa cggcaagggc gtaccgtgat cctggtcacg
     5581 cacgacatgg gtgcaattag gcaatactgc accagggcta tgatgattga caacggaaaa
     5641 atagttaaag aaggcgatgt ggagaaagta gcccaagctt acttagctct ttttagcgaa
     5701 gaaaataaaa ccaagcaaag taaaagtgaa gatgatgata ggtggggtaa tggtcttcaa
     5761 actaccacca gggcgaaggt aaagactaca gacgaagaaa taattattac aaccgagttt
     5821 accgcgcatg cccacataga gtctgttatg tacggcttca ctttgtacga gaaggcaagc
     5881 aaaacaaaca tttttgagag caataccaag cggctgcgcc taaagatgcc tccagtcgtt
     5941 gagaatgaga taactacgct cgaatggaga gcgcccaaca tccttaggaa tggtgaatat
     6001 gcaatcagct catcaacttg tgatgaatca accttgactt actacgaatg gcttaatggc
     6061 gcagacacat tcaagattag taacaaagtt gaaactgctg gagctgtaaa ccctgttgtg
     6121 aagcttaaag ttacgagtaa aaaggatgac atcaaaggct aaaacatgaa gagacagatc
     6181 tataatacgg gtttacgtac agctaacgct gcattaaatg cgaacccaaa agtaaagcgt
     6241 aaagtgaagg gatttctcct ggcaacagtg ggcgcgcagc attggggaag atttaagtat
     6301 cggcaatggc ttgatagaaa cttcccagac tttattgaaa ttgccaaact caggaagatt
     6361 gatcagtcat taaaatacag gccacttatt tctgtggttg tccctaccta tagcccaaag
     6421 ccgcagttcc taagggattg catagaatct gtgcaaggac aaatctatga aaattgggaa
     6481 ctatgtattg ttgatgatgc atcgcctgac gaaacgactc gagaaataat aaaagaatac
     6541 tctgcaaacg atgaccgcat ttcatttaag tttcttgaaa caaaccagca catcgcccaa
     6601 gccactaacg aggctatcaa aatggcaaaa ggggatttcg tagcgctctt tgatcatgat
     6661 gatttgctgt ggccgaatgc cctgctagaa gtagtacggg cgcttaacaa caatcagaaa
     6721 ttggatttta tctatagtga cgaagacaag ataactgaag atagatttgt gcaccctgga
     6781 gcatttttta agcccgactg gagccctgac ttcttacata gcgtaaacta tattacccac
     6841 ttgtcggtag tcagaaagag cctgctagat aaactcgggg gcttaaatgg agagtataac
     6901 ggagcgcagg actgggattt attcctgcgc atcacgagca ctacaaaaaa catctaccac
     6961 atccccaaag tcctttatag ctggcgcgtc cacgacctct caactgccaa aactacgaaa
     7021 tctaagccct acgtcattga agcacaaaga aaggctatta ctgccgattt aaaacggcga
     7081 gggtacgaaa atgccacagt ggaacaagac cccaaacatc aaggatactg gcatgttgaa
     7141 cagcctataa aaggaaatcc ccttatatca attgttatcc caaccaaaaa tcagtacaag
     7201 gttgtgaagc gctgtgtgaa ctccatcttt aagaaaacta cctataacaa tttcgagatt
     7261 gttcttgttg atacgggcag tactgataaa aaagttcgaa gttggtacaa gaaacttcag
     7321 caaaagcaca ctaacgtacg gatcattaat tggcccgaac agccttttag ctattcacgc
     7381 agttgcaata aaggtgccca ggcagctaag ggtgaacttc tagtgatgct caataatgat
     7441 acagaagtta ttacaccgaa ttggctcgaa ctgctggccg gtgacgccca gcgtccagaa
     7501 ataggagccg tgggatgtct gttattcttc ccggacggac gacacatcca gcatgctgga
     7561 ataggcgttg gcctgggtgg agttgcagca aactcatttt ctatgatgac gctcatgcag
     7621 ccaatgaccc agacgcagca cctcatgatt aatactaagc acaacatgtc ggcagttacg
     7681 gcagcttgca tgatgatccg aacaagcgtc ttcgaagaag taggtgagtt tgcagaagaa
     7741 tttagggtca cctataacga tgtagactta tgcctacgct tgcgcgaaaa aggctacgaa
     7801 atcctctata ctccatacgt tcggcttatt catcatgagt caataagcgt cggtatgcct
     7861 gaagaaatca agaagcgtga caccagtgaa tttcgcgaag cgaaagagtt atttgtaacg
     7921 cgctgggcta attatgtgca gcatgaccct catctaaacc ccaacttaag caaagatgat
     7981 gcattctatg atgttccgga tattgctgat cttagcaacg acagcaaaat gtcacaggaa
     8041 gataaacctg ctatcgaacg tcgcttgcgt cgtcgtaaat tatttgtcaa ctcaccataa
     8101 gtgaggcgct tattgataac tacaaaaaat actttaaact taaacggaaa gctcactaag
     8161 ttcatgccta aagcaacaaa tcgcgcacct gccaaaacaa caaaatggtg gcataatcca
     8221 agcctaatat ttttaatgct cggagcaata ttcggaatca cttttgtcac aatcaatccc
     8281 cctggctttg gattggatga gcgggcacac tttctccgtg cctatgaact gtcacagggc
     8341 cactttctaa caactcgaac aggagtcgac cagcatattg gcgcccggat accggcaaac
     8401 ataggacaat gggttcacga cagcaccatt aatctactga gcgttaacac gtctgcaagc
     8461 ctcagtaatc gacatgacgc cgatactaaa ctatatacca agtattatca tcagcgtctt
     8521 tctaaggaaa cagttgatga ttttgccact aatcagcagc ttgccggtac ctttaattat
     8581 agccccctct cttacctccc cttagctgcg ccagtgtggg cgggcataaa attacacatg
     8641 aactttatgt ccatacttta tcttgcgcgt ataacaaatc tgctactgta tctccttgtt
     8701 gtctacattg cgatacgtat agcaccccgc tttaaatggc tgatttttat agttgctttg
     8761 ttgcctacag ccgtattcca agcgtcttca gtttcgatag atccgataat caattcttta
     8821 tcgtttttat tattagcctt atttttactt atttggcagc aggctcccaa acctatctct
     8881 ttgcgtcagg cattcattac ccttagtatc ctaggtttct tagcgatttc taaacagcct
     8941 tatgctttat ttgggttaat gttcttagct ctgcctcgag cggcgtttgc taacaaaaag
     9001 agcgaaatag catacaaact gacatgggca agcaccccgc tgctgcttgc tattatatgg
     9061 tctgtgctcg atcgtaatac cataaaacag ctatcaaccg agtacttgca ggcgaacgtt
     9121 tctacggtgc aacagataaa atttattatt cacgggcctc ttagctttgc tgcagtaatt
     9181 gcacgaacat attatgaaaa gagcggggcc tttttagcaa ccatgattgg ggctatcggc
     9241 gataggtccg ttggtctatc taacctaatt ctggttcctg ctgcggcttt attaatgggc
     9301 ttaggcgtaa tccatccaag ctcagacatc gcaccgccta ggcgcaaacg ctggacggcc
     9361 gcttggctat tagtcgttgc ccttggagtt ataggggcaa taacggcagc tctttatatc
     9421 ggagcctcta atgttgccgc cagagtagtc tacgggatcc aaggacgata cttcatacca
     9481 gtgttgccgt ttataggagt tgcagtctca attctgctac gtgagagagt tagggttagc
     9541 cgggctctga ttgagaatac aatgatagca ggaagttccc tgctgctact aggaacgaca
     9601 atcttttact acattgtaaa ttattagaga caagccctgc atccgaattt actcaggctt
     9661 actgtccgtt ccagtattgg gcgatcatgc cgatacccgc cacttcattg gtgtcccacg
     9721 tcccaccagc gggtagaccg gacacggtgc cggccacagt aaagttagct ccattttgag
     9781 gctgaccgtt agtatttgtc gctgtaaggc catagaagtt gaacgacgac gtattccaag
     9841 agccgttaag tccacaatag gttgggcaag tatctccggc cgagatctgg gtgttggtaa
     9901 atgagtcatt gaccgaatca ccgtttacac gagcatttcc ctcgacattg aagaagagcc
     9961 ttggaaccag ggtggcatca atatccgcca cagcagtatt cccttcgtag aaaacggctg
    10021 tattgtcgcc cgaccaccac ttcagtgtta caggctcatt ggtattactt gctgctggcc
    10081 ctaggtagtc gtaggtaaag gttccgtttt gcaccaattc ccagatgtag tatggctgcc
    10141 catgtgattc aggctgggac gaatcagttt ggataccaac agagatggca ttgttgctgc
    10201 catcgtgggc attgatgtag gaagagtaac cagttcctga acccgcttca ttcatcgtag
    10261 cggtatatgt aagatatggt gagccagcgg gaatggttac aggttcagca gttattgagc
    10321 cggttgagaa tgaaatgttg gtaaaagttg tatcggctat gtcgccggta tttcggccag
    10381 cagccaagaa tgaaatcgaa gggttattct cggcaaccgg gtacggccct aaagttacat
    10441 tgttatcaat cgttacagaa atgcccccag cggtccagtt gaaagtaaat aggtgcgacg
    10501 taccagatat cccgttgttg ccccaatagc cgccgactgg tctgccaccc gcagcacctt
    10561 cgatcataag cgtggtgcca gtgggcgaga caccaggatc gtgaataagc ccgaaagcaa
    10621 tatagtttcc gggatcattc cagaattgta ccaatccctt gtaccccgaa ccacttccat
    10681 agctgcaccg gcccccagga cagcaggttc cattaaggcg tacattaagc gttccagaag
    10741 aagatccgcg actggtggtg gtaacgcctg attgatagtt aaccgaaccg gttccccagg
    10801 cac
//
LOCUS       DATNGP010000182         2168 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_4214812, whole genome shotgun sequence.
ACCESSION   DATNGP010000182 DATNGP010000000
VERSION     DATNGP010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2168
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4214812"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1436
                     /locus_tag="VN778_04730"
     CDS             <1..1436
                     /locus_tag="VN778_04730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HXR50302.1"
                     /translation="DWNGLAADIDAGQVTGKKIERPKWHLKNLRLLGNRKYPMRPSEL
                     THAVIIATVNESREVLEPTIQAILAADYDPKQIILVMAYEGRAGKPAEQRVKELTKLY
                     GGQFKHAMAVKHPPNLPGEIVGKGGNITYAARKLQKFLEQKKIDTTKVLVTTLDSDNR
                     PDKRYFAALSYLYCVVPDPLKASYQPLAMFTNNIWDTPNFMRIIATGNNLFYIVNTQR
                     PHLSRNFSAHAQPMSSLIEMDFWSTRTVVEDGHQFWRSYFHFDGDYRVYPFSIPIYQD
                     AVLAEGYKRTIRAQFYQLRRWTYGASDVAYIADKGFWHKNAVPKADVLAKLLRTLWDH
                     VNWATGALMIWYIGFVPHLIHPQNYGSNVIPLEVQTLQRVGMIAFFVTIYICLVTLPP
                     RPARYKRHRSLFMLAQWVLLPLSSISFGSLAAFNSQTRLIFKKYLSKFDVTEKTVITE
                     SGKKVTTAADPTRQTRRSRLFALFDKK"
     gene            1449..2054
                     /locus_tag="VN778_04735"
     CDS             1449..2054
                     /locus_tag="VN778_04735"
                     /inference="COORDINATES: protein motif:HMM:NF014453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HXR50303.1"
                     /translation="MTQGVPYAFGPEKRLFDIGFAGLFLPVAYGARTLLSHTLKDMSV
                     PDNNPDFYQERSGVNREPFGLHKLRTLNSETGEPLNRLAEFMRKNGIDETIQARQVWR
                     GVMSAVGRRPVQPREEEAILSNGSPKLVAQYERIVLPTKTGMIDSFCILNHSSPNKIE
                     TMIANADLKLEMNIEDVLRGSWLNDASIFATALRAAAQGRL"
BASE COUNT          513 a          599 c          570 g          486 t
ORIGIN      
        1 tggactggaa tggcctggcg gccgacatcg atgcgggaca ggtaaccggc aagaaaatcg
       61 agaggccgaa atggcacttg aaaaacctgc ggctgctagg caaccgcaaa tacccgatgc
      121 gccccagcga actgacgcat gctgttatca tcgccaccgt caacgaaagc cgggaagtgt
      181 tggaaccgac aatccaggct atcctggcgg ccgattacga ccccaagcaa attattctgg
      241 ttatggctta tgagggccgc gctggcaagc cggccgagca gcgtgtcaag gagctgacca
      301 agctttacgg cggccagttc aagcacgcca tggcggttaa gcaccctccc aacctgcctg
      361 gcgaaattgt cggcaagggc gggaacatca cctacgcggc ccgaaaactg caaaaattcc
      421 ttgagcaaaa gaaaatcgac actactaaag tgctcgtgac cactttagac tccgacaacc
      481 ggcccgataa gcgctacttc gccgccctaa gctacctata ctgcgtcgtg cccgacccgc
      541 tcaaggcgtc ttaccagccg ctggccatgt tcaccaataa catctgggac acgcccaact
      601 tcatgcgcat catcgccacc ggcaacaact tgttttacat cgtgaacacc cagcggccgc
      661 atctgtcacg taacttctcg gcccacgcgc aacctatgag ttcgctgatc gagatggatt
      721 tttggagcac ccgcacggta gtcgaagacg ggcaccagtt ttggcgctcg tacttccatt
      781 ttgacggcga ctaccgcgtt taccccttca gcatcccgat ttatcaggat gccgtgctag
      841 cagagggcta caagcgcacg attcgggcgc aattttacca gttaaggcgt tggacatacg
      901 gcgcctctga cgtggcttat attgccgaca aaggtttttg gcacaagaac gccgtgccca
      961 aggccgacgt gctagccaag ctgctgcgca ccttgtggga ccatgtcaac tgggcgaccg
     1021 gcgcattaat gatctggtat atcggttttg tgccgcacct tattcatccg cagaattacg
     1081 gctcgaatgt cattccactg gaggtgcaaa cgctgcagcg agtgggcatg attgcctttt
     1141 ttgtcaccat ttacatctgt ttggtgacac tgccgccgcg cccggcccgc tacaagcgcc
     1201 accgctcgct ttttatgctc gcacagtggg tgctgcttcc attgtcaagc attagtttcg
     1261 gttcgctggc ggcgttcaat tcccaaaccc ggctgatttt caagaagtac ttgtccaagt
     1321 ttgacgtaac agagaagaca gtcataaccg aatcaggcaa aaaggtaacg acggcggccg
     1381 acccgacgcg ccaaacacgc cggtcccgct tgtttgcttt atttgacaaa aagtagtata
     1441 ataagtaggt gacccaaggt gttccatatg cattcggtcc tgagaagaga ctgttcgata
     1501 tcggctttgc cggcttgttt ttacctgtcg catacggtgc caggaccttg cttagccata
     1561 ccttaaaaga tatgtccgtg ccggataaca acccggattt ttatcaagaa cgctccggcg
     1621 ttaatcgtga gccgtttggc ttgcataagt tgcgcacgtt aaatagcgaa actggcgagc
     1681 cgctcaaccg gttggccgag ttcatgcgca aaaacggcat cgatgaaacg atacaggcca
     1741 gacaggtttg gaggggcgta atgagcgcgg taggacgtag gccggtccag ccacgcgaag
     1801 aggaagcgat tctcagcaat ggctcgccca agctggtggc tcaatacgaa cggatcgtgc
     1861 tgcctactaa gaccggtatg atagactcct tttgtatctt gaaccattct tctcccaaca
     1921 aaattgaaac tatgattgcg aatgccgacc tgaaactaga gatgaacatc gaggacgttc
     1981 ttaggggctc atggttaaac gatgcttcaa tatttgccac tgccttgagg gctgcggctc
     2041 agggcaggct ctagagccta acgtcttggt ggcgtacctg gtaatgcatg ctggctgcct
     2101 tgtcgaatcg gtttagtgtc acttgggtaa cctgagcgat tgagccggac cacacccggc
     2161 cgttgttt
//
LOCUS       DATNGP010000183         3853 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_2361214, whole genome shotgun sequence.
ACCESSION   DATNGP010000183 DATNGP010000000
VERSION     DATNGP010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3853
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2361214"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1045)
                     /locus_tag="VN778_04740"
     CDS             complement(<1..1045)
                     /locus_tag="VN778_04740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50304.1"
                     /translation="MPNKKNSEPEQTPEEKALAERVDAMLDPNRPDQSPALQSQSSSA
                     PAASDHPLDIFEGKSAPIPISTKDASDTPAEPTKTAPELPGKASKQASVESPKSEAEP
                     ASEPAEPQPEEPAPPAPDIEDTNAQLDDSETDKAVDEIAANEADALLAAEDAAKAGRV
                     VTKKAAPRRSWKSKLVALLKNKWTWIGLALILVIILALPMTRYKLLGLVIKEPVTVRV
                     LDSKTNTPVSNADVTLGGASSKTNAEGTATLRAALGTSQLQVTKQYYKTYALKYSVSF
                     KAASTSVNLVATGRQVPITVLNKITGKPLQGAEIKVLATTAKTDSQGKATIVLPTTAA
                     SDNAVISLNGYNIA"
     gene            complement(1289..2269)
                     /locus_tag="VN778_04745"
     CDS             complement(1289..2269)
                     /locus_tag="VN778_04745"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HXR50305.1"
                     /translation="MKTRVLGGELTVSMIGLGCMGISAFYGQATEREAVDVIHYALDN
                     GVTFLDTADIYGPETNEKLVGKAIQGRRDQVVLATKFGNEIDDGGNRTGKVNGRPEYV
                     HKCIDRSLQRLGVDHIDLYYQHRVDPEVPIEETVGAMAELVKAGKVRYLGLSEAAAAT
                     IRRAAKVHKITALQTEYSLWTRDPEGEILDTVRELGIGFVAYSPLGRGFLTGRWQKPE
                     DLPADDWRRQNPRFQGENFSKNLELVDKVKEIAAEKQVKPGQLALAWVLARGKDIVPI
                     PGTKHIEYLKENIAATEIELTDEDLRRLDEAAPKGTAAGDRYPDMSTVNR"
     gene            2381..3010
                     /locus_tag="VN778_04750"
     CDS             2381..3010
                     /locus_tag="VN778_04750"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HXR50306.1"
                     /translation="MTFKESLQQAADYLAGHDPVLKPVIERAGRPTLQPHKNYYWELI
                     DSIISQQLSVKAATSIEKRFRELFGTDFPAPLAILEKSIEDLRAVGLSRPKARYIQDL
                     AQHILDGQLKFDKFDRQTNDEIITELIAVKGIGEWTAHMFLIFCMGRLDVLPTGDLGI
                     RNGIRQLYNFKDAPTPDQIRSLAQKNRWHPYESVASWYVWRSLGNTPAV"
     gene            complement(3007..3387)
                     /locus_tag="VN778_04755"
     CDS             complement(3007..3387)
                     /locus_tag="VN778_04755"
                     /inference="COORDINATES: protein motif:HMM:TIGR01550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HXR50307.1"
                     /translation="MTSYLALEEILQLHHKIIEDFGGSHGVREEQRLLSVVEAPKQSV
                     FGQEQYPTIFEKAAVYLRNIIGDHPFSDGNKRTGVTVCGIFLKRNHEKLTATPKELED
                     FAVQVAVEHLDVSAVAAWLQAHSV"
     gene            complement(3384..3605)
                     /locus_tag="VN778_04760"
     CDS             complement(3384..3605)
                     /locus_tag="VN778_04760"
                     /inference="COORDINATES: protein motif:HMM:NF013561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HXR50308.1"
                     /translation="MASKTINISLDEELIKKIDKAAKAEYSSRSDFIRSSVVEKITAK
                     QGEPRSSIDNDYAEFIKQYGQTLKNLADR"
BASE COUNT          848 a         1071 c          938 g          996 t
ORIGIN      
        1 tggcgatatt gtagccattt aagctaatca ccgcattatc gctcgctgca gttgttggca
       61 aaacgatagt tgccttgccc tggctgtcag ttttcgcagt cgtagccagg actttgatct
      121 cggcaccctg cagcggtttg ccagtgattt tgttcagtac cgtaatcggc acttgccgcc
      181 cggtggcgac caagttaacg ctggtgctag cagccttgaa actcacagaa tattttaagg
      241 catacgtttt gtagtactgt ttagtgacct gtaactgaga cgtccccagc gccgccctaa
      301 gcgtagccgt gccctcggca ttcgtcttgg acgacgctcc gcctaaagtt acatcggcat
      361 tactcaccgg ggtgtttgtc ttgctgtcca agacccgcac cgtcaccggt tctttgatga
      421 cgagacctag gagtttgtag cgtgtcattg gcagcgccag aataataacc aggattagcg
      481 ctagccctat ccaggtccac ttgtttttga gaagcgctac cagcttggat ttccaacttc
      541 gtcgaggggc tgctttttta gtaactaccc ttccggcttt ggcggcatct tcggccgcga
      601 gtaaagcatc ggcttcgtta gccgcgatct cgtcaacagc cttgtcggtt tccgagtcat
      661 cgagctgagc gttggtgtct tcgatatcgg gcgctggcgg tgccggttct tctggctgcg
      721 gctcggccgg ttcagaagca ggctcagctt ccgattttgg agactcaacg ctggcttgct
      781 ttgacgcttt gccgggaagt tccggcgcgg tcttggtcgg ctccgccggt gtgtcggaag
      841 catccttggt gcttatcggt attggcgcgg attttccttc aaaaatatca agcgggtgat
      901 cgctggctgc cggtgcactc gatgattgag attgaagggc cgggctttga tccggccgat
      961 tcggatccag catcgcatcg acacgctctg caagcgcctt ttcttcaggt gtttgctccg
     1021 gttcagagtt tttcttatta ggcatatgct tatccgaatt taagtctatc aaaaaaccag
     1081 ccagccgacc atcgactgac tagtttttga aggcagacgc aacaactacg acaaatgccg
     1141 cagtacgctt agtgcgaaag cattgaaccg tttcttagga ccgagataca aaaagctcat
     1201 tggaggctaa gaccctttat attttataaa ccactcttcc aatcgtagct caggctatgg
     1261 tcatcgtcaa caggcttttg tagctttttt aacgattaac tgtggacatg tccggatacc
     1321 gatcgccagc ggcagttccc tttggggcag cttcgtctaa acggcgtaag tcttcgtcag
     1381 tcagctctat ctctgtggca gcgatatttt ctttcaaata ttcgatgtgt ttcgtccctg
     1441 gaatcggtac gatatctttg ccccgggcca gtacccaagc caaagctagt tgacccggct
     1501 tgacttgttt ctcggccgca atttctttga ccttgtcgac cagctccagg tttttggaga
     1561 agttttcacc ttggaagcgg gggttttgac ggcgccaatc atccgccggc aaatcttcag
     1621 gcttttgcca acgcccggtc aggaacccgc ggccgagcgg gctgtacgct acaaagccga
     1681 tccccagctc gcgcacggtg tccaggattt cgccttccgg atcgcgagtc cacagcgaat
     1741 actcagtctg caaggcggtg atcttatgaa ctttcgccgc ccggcgaatt gtggcagccg
     1801 ccgcttcact caggcctaga tagcgcacct taccggcttt caccagctcc gccatagctc
     1861 cgaccgtctc ttcgatgggt acctccggat caacccgatg ctgatagtac agatcaatat
     1921 ggtcgactcc gagccgctgc aaactccgat caatgcattt atgtacatat tccggccggc
     1981 cgttcacctt gccggtccga ttgccgccgt catcaatctc attgccgaat ttcgttgcca
     2041 gcaccacttg gtcacgccgc ccttgaatag ccttgccgac cagcttttcg ttggtttccg
     2101 gcccgtatat atcagccgta tccaaaaaag taacgccgtt gtcgagagcg taatggatga
     2161 catcgaccgc ttcccgctca gtcgcctgac cgtaaaaagc acttatgccc atgcacccta
     2221 acccgatcat agaaacagtt aactcgccgc ctaagacgcg cgttttcata ttcacctccc
     2281 ttgtctttcc caattactct agcagcttag gcgctccgct agctgtaagc ttttccgcta
     2341 gggtcatccg cggataaata ataggttata taatgcatct atgactttta aagagagttt
     2401 gcagcaagcg gccgactacc tggcagggca tgatccggta ttaaaaccgg tcattgaacg
     2461 cgccggccgg cccacgctac aaccgcacaa aaactactac tgggagctca tcgatagcat
     2521 catcagccag cagctcagcg tcaaagcagc caccagcatt gagaaaagat tccgtgaact
     2581 gttcggcacc gactttccgg caccccttgc catcttagaa aaatccatcg aggacctacg
     2641 cgccgtcggc ctcagccggc ccaaagcccg ctacatccaa gatctggctc agcacatcct
     2701 cgatggccag ctcaagtttg ataagttcga ccggcaaacc aatgacgaaa taattaccga
     2761 attaatagcc gtcaaaggta tcggcgaatg gacagcgcac atgttcctga ttttttgcat
     2821 gggccgctta gacgtcttgc ccaccggcga cctcggtatc cgcaatggca tccgccagct
     2881 ctacaatttc aaggacgcgc ctacgcccga ccaaatccgc tcgctcgctc aaaagaaccg
     2941 ctggcacccg tacgaatcag tcgccagctg gtacgtctgg cgcagcctcg gcaacacacc
     3001 agccgtttaa acggaatgtg cctgcagcca agccgcaacc gccgacacat ccagatgctc
     3061 gacagccact tgcaccgcaa aatcttccag ctcttttggt gtagcagtta atttctcatg
     3121 attacgcttc agaaaaatac cgcacaccgt tacaccagtc cgcttgttcc catcactaaa
     3181 tggatgatca ccaatgatgt tgcggagata gaccgccgct ttttcgaaaa tagtcgggta
     3241 ttgttcctgg ccaaaaaccg attgcttggg agcttcgacc acagagagca ggcgctgctc
     3301 ctcgcgaacg ccgtgagagc cgccaaagtc ttcgatgatt ttatgatgca gttgcagaat
     3361 ttcttcaagc gctaagtagc tagtcatctg tcggccagat ttttgagggt ttggccatat
     3421 tgtttgatga attcagcgta atcgttgtcg atacttgagc gtggctcgcc ttgtttagcg
     3481 gtgatttttt ccacaacact actacgaata aaatcgctgc ggctactgta ctcagctttg
     3541 gcggctttat cgattttctt gataagctcc tcgtcgagag agatattgat agttttactt
     3601 gccatacaaa aactatacaa aatttgtatg tattatgtca acatggtttg gccgctattg
     3661 gtacctagta catccgccag attttatggt gccacgtccg cgggataatc gagaggcact
     3721 cggtcggata cggttttttg gccggggaaa tcatcggaga gccgacgcag gtcctcggga
     3781 gctagctccc agcctaaggc gccgagattt tcttccagat gtgtgacact tgtggtctta
     3841 ggaatggtta tga
//
LOCUS       DATNGP010000184         2266 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_2264693, whole genome shotgun sequence.
ACCESSION   DATNGP010000184 DATNGP010000000
VERSION     DATNGP010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2266
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2264693"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..90
                     /locus_tag="VN778_04765"
     CDS             <1..90
                     /locus_tag="VN778_04765"
                     /inference="COORDINATES: protein motif:HMM:NF014796.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HXR50309.1"
                     /translation="DYFNRRPDYIQAWWHIVNWPEVERRFAEH"
     gene            111..1487
                     /locus_tag="VN778_04770"
     CDS             111..1487
                     /locus_tag="VN778_04770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXR50310.1"
                     /translation="MEVKEVRRKLLVLIATIFGSAVVFLDGTIVNLALPKIAANLHVG
                     FSAQQWISDGYLLTLSALILLGGSLGDIFGRKKVYILGLVGFGVTSLLCALAPNSVSL
                     ILARVVQGLFGALVVPGALSIINTNFPQAERGQAIGRWTAWTSAFFVLGPLLGGIILD
                     VAIWRWIFLINLPLIAICLILALPSINESRDEGLRHIDYPGAILAALALAGVTYGLIQ
                     GPTQHWRAADIITLVAGAFMSGAFVWWEARSRDPMVKLSLFKSRNFSGSNLMTFAMYG
                     ALGGLTFALVIYLQSTMHYSSLKAGMSLLPVSIIMFAFAARVGKLSSVYGPRIFMTVG
                     PIVAGLGIATFYFVSPGDSYWLSVLPGALLFSCGLVLTVAPLTTTVMTSVDEHSSGIA
                     SGINNAVSRAAGLIVVALLGLLGTHNFYHFSMALCAGLAIAAGFISYVVVVNPPRLAP
                     KGQEHMVK"
     gene            1491..>2266
                     /gene="guaA"
                     /locus_tag="VN778_04775"
     CDS             1491..>2266
                     /gene="guaA"
                     /locus_tag="VN778_04775"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HXR50311.1"
                     /translation="MESTTETLLIIDYGSQYTQLIARRARELGVFSLIVPFTMPFEEV
                     EQLQPRGIILSGGPNSVYEKDAPGLDKRLLSLDVPVLGICYGLQLITQALGGAVEASV
                     TREYGLAAIKLGGGSQLLDKKIDASAIWMSHGDHVKVPPTGFVVTALSGEIICGIENA
                     DKHIFGIQFHPEVTHTKHGSRILKTYLRKICHFSLDWQPTSIIDHQIKQIRQRAGKDK
                     VICALSGGVDSTIAATLVDRALGKQQTCIFVDNGLLRENE"
BASE COUNT          473 a          594 c          605 g          594 t
ORIGIN      
        1 gactacttca accgtcggcc cgactatatt caggcctggt ggcatatcgt caactggcca
       61 gaagtcgagc gccgcttcgc cgagcactaa cggactgtta caattaggga atggaggtca
      121 aagaagtccg gcggaaactg ctcgtcctga ttgcgaccat ttttggcagt gcagtcgttt
      181 tccttgacgg caccatcgtc aacctcgctc taccaaaaat cgccgccaat ttgcatgttg
      241 gattttccgc gcagcaatgg atctctgatg gctacctcct gacgctctcc gcgcttattt
      301 tactcggcgg ttcactcggt gacatctttg gacgtaagaa agtatacatc ttgggcctgg
      361 ttggctttgg agttacctcc ttgttgtgtg ctcttgctcc aaattccgtc tcacttattt
      421 tagcgagagt agtgcaagga ttattcggcg ctctggtggt accgggggcg ttgtcaatca
      481 tcaacactaa ctttcctcag gccgagcgcg ggcaggcgat tggtcgctgg acggcctgga
      541 ccagtgcctt cttcgtactc ggccccttgc tagggggtat aattctggac gttgctatct
      601 ggcgctggat ttttctgatc aacttgcccc tgattgcgat ctgtttaatt ctggctctgc
      661 catccatcaa tgaaagccgc gacgaaggcc taaggcatat tgattatccg ggagcgattc
      721 tggcggctct ggcactagcc ggtgtcacct acggactcat tcaaggcccg acccagcatt
      781 ggcgcgctgc cgacatcatc acgctggtag ctggagcttt catgagcggt gctttcgtgt
      841 ggtgggaggc gcgcagccgc gaccctatgg tgaagctatc gctcttcaaa tcccggaatt
      901 tttctggctc aaacctcatg acttttgcca tgtatggagc tctcggcggc ctaacttttg
      961 ccctcgttat ttacctgcaa tcaaccatgc attactccag cctaaaagcc ggtatgagct
     1021 tattgccggt cagtatcatt atgtttgcgt tcgctgcccg ggtaggtaaa ctgtcgagtg
     1081 tctatggtcc cagaattttc atgacggttg gtccaattgt ggctggttta ggcattgcta
     1141 ctttctattt tgtcagtccc ggtgacagtt attggctgag tgttttgccg ggagcgttgc
     1201 tattcagctg cggcctagta ctgacagtag ccccgctgac taccaccgta atgacgtcgg
     1261 tcgacgagca tagttcaggc attgcttcgg ggatcaacaa cgctgtctcc agagccgccg
     1321 ggctgattgt cgtagccttg ctcgggctcc tgggtactca taatttctat catttctcca
     1381 tggctttgtg cgcgggactg gcaattgctg ccggctttat ttcgtatgtc gttgtcgtga
     1441 atccgccacg cctcgcacct aaaggtcagg agcacatggt aaaataaatc atggagtcaa
     1501 caacagagac gttgcttatt atcgactacg ggtcgcagta cactcagctg attgccagac
     1561 gggcacgaga gctgggtgtt tttagtttga tcgtgccctt tactatgcca tttgaggaag
     1621 tggagcagtt gcagccacga ggaattattc tgtccggggg gccgaattcg gtgtatgaga
     1681 aagacgcgcc aggactggat aagcgcttgc tctcgctgga cgtgccagtc ttgggaattt
     1741 gttacggtct gcaactgatc acacaagcat tgggtggcgc cgtagaggct tcggtcaccc
     1801 gcgaatacgg cctggcggcg atcaagctcg gcggcggtag ccagctgctt gacaaaaaaa
     1861 ttgacgccag tgcgatttgg atgagccacg gcgaccacgt caaagtcccg ccaaccggct
     1921 ttgtcgtgac cgccctgagc ggcgaaatta tctgtggcat cgagaatgct gacaagcata
     1981 tttttggtat acagttccac cccgaagtca cccataccaa gcatgggagc cgtatcctca
     2041 aaacatactt gcgtaagatc tgtcactttt cgctggactg gcagcccacc tcgatcatcg
     2101 atcatcagat caaacagatt agacaaagag cagggaaaga taaggtgatt tgcgcgttgt
     2161 ccgggggtgt cgactccaca attgccgcga cccttgtcga ccgcgccctc ggcaaacagc
     2221 aaacctgtat tttcgtcgac aatggcctac tgcgcgaaaa tgaata
//
LOCUS       DATNGP010000185         4335 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_1508886, whole genome shotgun sequence.
ACCESSION   DATNGP010000185 DATNGP010000000
VERSION     DATNGP010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4335
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1508886"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1061)
                     /locus_tag="VN778_04780"
     CDS             complement(<1..1061)
                     /locus_tag="VN778_04780"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding domain-containing
                     protein"
                     /protein_id="HXR50312.1"
                     /translation="MNIVASRLSNQHLVGESWTCVEDVVVNSGAVQAQDYNGAAWALS
                     LRLANATKADIDQAFNKGRILRTHVLRPTWHFVLPEDIRWMVKLTAPRIKQAMSYYNR
                     KLELDDAFFARCHKIITRALANDAWQTRAELAQVLAENGIEASGQRLGHIVMQAEIEG
                     LICSGPFSGKQFTYALIADRAPQAKALSREEALALLATRYFKGHGPASLQDFTWWSGL
                     TVADSKLALNLVKDQLDHETYDGKEYWFSGALAEKIAPQILLLSVYDEYVIAYQNYGP
                     IFLDKTKHLTNIFGNARLDYVIVKDGIVIGTWRRQVNKNKVALEVKLEIKLDPEDNNR
                     LLVAAENYSRFLGLPVEIA"
     gene            1132..1704
                     /locus_tag="VN778_04785"
     CDS             1132..1704
                     /locus_tag="VN778_04785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50313.1"
                     /translation="MTIQELFIRSNEALRKSIDHIAEDQWDLAMPPGTPSKPTNLKDA
                     VRYHTYDDAWVPDVLAGKSKEEVGDVYEPILKKEDIKASYDRYNERAIEDVQAFTELD
                     RTVHLSYGDFPARDYLQHIISFRAFRSYDIAKLIGADTTMADDFVEALLAEFSPVVEG
                     YRHMGVFPPAVEVADDASPQVKLLALVGRR"
     gene            complement(1751..2104)
                     /locus_tag="VN778_04790"
     CDS             complement(1751..2104)
                     /locus_tag="VN778_04790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50314.1"
                     /translation="MEKLSQSRLASLLTLIVGAWMMLTPLAISMTGAALTSILITGAI
                     IIVASLVQLGWMNTLPSWVNALAAVWLFISAFAFTMGTGAAWNQAIFAVITFVLAAWD
                     GVEMGEVHRGQQMHA"
     gene            complement(2248..2508)
                     /locus_tag="VN778_04795"
     CDS             complement(2248..2508)
                     /locus_tag="VN778_04795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50315.1"
                     /translation="MNTSTNSSLYDELVNVTHQYFGPAANRFVTRQISNHLSKDPELL
                     KKKDLKALIDWISVAMALLVEDEKLVNKYIADLKELADGSSK"
     gene            complement(2514..3392)
                     /locus_tag="VN778_04800"
     CDS             complement(2514..3392)
                     /locus_tag="VN778_04800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50316.1"
                     /translation="MYSYIALLALYAGFRLLPAPSPATLLLYHVSSTELRFIELTIIL
                     LSAGIWYAGFYGYDRLDTYTKYIKNDKDGQQVAWLTKGIFVLVMWLPVSSVISSVLQY
                     IAMRHLGLLTAVTIIDNYISLLFPLVGFALIGVGARGLSTLVKRRFTYPAANILVIIL
                     IYIGLIYYHLVASTYHRSSIYHMSVWLILVTLVAPYMYMWYIGLTAAYEIYHYQKKVA
                     GIVYRSSWNLLALGLGWLIVTSIGLQYLTTLSARLTKLSIYWILAIVYTLLIVLSIGF
                     VLIALGAKKLQKIEEV"
     gene            complement(3577..>4335)
                     /locus_tag="VN778_04805"
     CDS             complement(3577..>4335)
                     /locus_tag="VN778_04805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50317.1"
                     /translation="TALERGDNVILGTNHFELTDIAKIFAGINVWLAKAGYEQLLEGD
                     SNVKRALIASQITSVMGIKMRNPDTTEGQPPTLTVPVTLALTALVHDLLKSYPNTPSI
                     AAIRKLWEAETTLNNLRVKGTVMSLQKAGRLFLAMAPSGSQDKPLEGDPTTYLLQPLT
                     QGTLDMMKQTRTYVLMMAMWNVEGKPIVRFSGREINGVWVPNGLASIKNDHQGNAEMT
                     NLSTTLNHWVPGLHFVYLPAVTRDSKKKRRSSDY"
BASE COUNT         1121 a         1078 c          977 g         1159 t
ORIGIN      
        1 caggcaatct cgaccggaag acctaaaaat cggctatagt tttcagctgc taccaaaaga
       61 cgattgttat cctcggggtc tagcttaatc tcaagcttga cttcaagagc aactttgttc
      121 ttattaactt gacgacgcca agtcccaata actatgccat cctttacgat tacataatca
      181 agccgggcat tgccaaagat attagtcagg tgcttggttt tatcgagaaa gataggaccg
      241 tagttctggt aggcaatgac gtattcgtcg taaactgaca ataataatat ctgaggtgcg
      301 atcttttcgg cgagcgctcc gctaaaccaa tattccttgc cgtcgtaagt ttcatggtcg
      361 agttgatctt tgaccagatt taaggctagc ttgctgtccg ctaccgttaa acctgaccac
      421 caggtaaagt cttgcagact ggctggccca tgtcctttaa agtagcgcgt ggcaagcaga
      481 gccaacgctt cttcccggct taaggcttta gcttgaggtg ctcggtcagc tatcagcgca
      541 taggtgaatt gcttgccact gaatggcccg ctgcaaatca gcccttcaat ttcggcttgc
      601 atgacgatat gcccgagacg ttgtccgctg gcttcgatcc cgttctctgc gagtacctgc
      661 gcaagctccg cccgcgtctg ccaggcgtca tttgctaagg cccgcgtaat aatcttatgg
      721 cacctggcaa aaaatgcgtc atcgagctcc agcttgcggt tgtaataact catcgcttgc
      781 tttatccgcg gcgccgtgag cttaaccatc cagcgaatgt cttccggcaa cacaaaatgc
      841 caggttggtc gcaaaacatg tgtgcgcaga atccgccctt tattaaaagc ctggtcaata
      901 tcagcctttg tggcattcgc cagccgcaaa ctgagcgccc aagctgcgcc attataatcc
      961 tgagcctgaa ccgctccaga attcaccacc acgtcttcga cacaagtcca tgactcaccg
     1021 accaaatgtt ggttagagag ccggctggcc acaatgttca tgcccatagc ataccttaat
     1081 ggacaaattt taagctaaaa cttatagtga ggaggaataa taggagaacg catgacgatt
     1141 caagaattat ttattcgatc taatgaagcg ctacgaaaat cgattgatca catagctgag
     1201 gaccaatggg atctcgctat gccgccaggc acccccagta agcccacaaa cctgaaagac
     1261 gccgtgcgtt accacaccta tgatgacgct tgggttccgg acgtgctggc cggtaaatcc
     1321 aaagaggagg tcggcgatgt ctacgagccg atattaaaaa aagaagacat caaggcttct
     1381 tatgatcgtt ataacgagcg ggctattgag gatgtgcagg ctttcaccga actcgatcgc
     1441 accgtacatc tatcgtatgg cgactttccc gccagagatt acctgcaaca tattatcagc
     1501 ttccgtgcct ttcgttctta tgacattgcc aaactcattg gcgccgacac tactatggca
     1561 gacgattttg tggaggcttt gctggccgag tttagtcctg ttgtcgaggg ttatcgacat
     1621 atgggggtat tcccgccagc agtcgaagta gcggacgacg ccagcccgca agtcaagctg
     1681 ctggccttgg ttggacgccg ctaagctacc gtagccacca cttggctgga gctcgtcgcg
     1741 ttgtaaactg ttaagcgtgc atttgttggc cgcgatgaac ttcgcccatt tcgacgccgt
     1801 cccaggcagc cagcacaaag gtgatgacgg cgaaaattgc ttgattccag gccgcaccgg
     1861 tgcccatagt aaaggcaaag gctgagataa acaaccaaac tgctgccaga gcattgaccc
     1921 agctcggcag ggtattcatc caccccagct gtaccaggct tgccacaata atgattgcgc
     1981 ctgttatcaa gatacttgtc aacgccgcac cggtcattga gattgccagt ggcgtcaaca
     2041 tcatccaggc tcctacgatc agggtcagca agcttgccag tcgtgattgc gatagttttt
     2101 ccataatgct tcctcctcag acttaagctg atttaagtta ttgcttttcg attcaacctc
     2161 ccttctattc atatggtaag tgttcgcaca ttggctcgct ataaataaat ttactccctg
     2221 agtgtgagtt taagcataga aagcttttta cttgctgctg ccgtcggcca gctctttcaa
     2281 gtcggctata tatttattga ccagcttttc gtcttcgact aatagtgcca tggccacgct
     2341 gatccaatcg atgagagctt ttaagtcttt cttctttaat agttcggggt ctttgcttag
     2401 atgattgcta atttgacgcg tgacaaaacg gttggccgcc gggccaaagt actggtgagt
     2461 aacattcacc aattcatcat ataaagagct gtttgtgctc gtgttcatgc gccctatacc
     2521 tcttcaatct tttgtagctt tttagccccg agcgcaatca gcacaaaccc gatagacagt
     2581 actattagta atgtataaac aatagcgaga atccagtaaa tggacaattt agtaagcctg
     2641 gccgataagg tagtcaaata ctgtaagccg atcgaggtca caatgagcca tccgagccca
     2701 agcgctaaaa gattccagct gctcctatag actataccag caactttttt ctgatagtga
     2761 taaatttcat aagccgccgt caacccgatg taccacatgt acatatacgg tgcaaccagt
     2821 gtgacgagaa taagccacac ggacatgtgg taaatactcg atctgtgata cgtcgatgcc
     2881 actaagtgat agtaaataag accgatgtag atcagaataa ttactaagat attcgctgcc
     2941 ggataggtaa aacgccgctt taccagtgta ctgagccccc gagcccccac accgatcaag
     3001 gcaaacccga cgagcgggaa aagcaagctt atatagttat cgataatggt taccgcagtc
     3061 agcagcccca ggtgccgcat ggcaatatat tgcagtactg aagatatgac cgatgaaact
     3121 ggcagccaca taaccagcac aaagatacct ttggttagcc acgctacttg ctggccgtct
     3181 ttgtcatttt taatatactt agtataggtg tctagtctgt cgtaaccata aaatccggcg
     3241 taccatattc cagccgagag caggataatt gtcaactcaa tgaagcgcag ttctgtagag
     3301 cttacatggt acagcagcaa agtagcaggt gagggagctg gcaagagcct aaatccggcg
     3361 tagagggcga gaagagctat ataactgtac aaaagttttg gtctcagctt caaccccatg
     3421 atccctccgg tcgcatcttc tgcggattgt tactttaggc tttcgttcgt taccacgtcg
     3481 ctggtcgtaa taataaaaca atggcgctct gtcaaaggtt aaaaacttta cagcagggaa
     3541 ctattttttc tgcatgagcc acagtgataa gaaattttag taatcactgc tgcgccgttt
     3601 cttcttgctg tctctcgtta ctgccggcaa gtatacaaaa tgtaaacctg gtacccagtg
     3661 atttaacgtt gtacttaaat tggtcatttc agcattgccc tggtgatcgt ttttgatgct
     3721 ggcaagtcca ttaggtaccc atacgccgtt gatttctcta ccgctgaatc ggacaattgg
     3781 tttgccttca acattccaca tggccatcat caaaacatat gttcgtgtct gcttcatcat
     3841 atcaagcgtt ccttgggtga gtggctgtag aaggtacgtt gtcggatcac cctccaatgg
     3901 cttatcctga cttccgcttg gagccatagc caaaaacaga cggcccgctt tctggaggct
     3961 cattaccgtt ccttttaccc gcagattatt cagcgtggtt tcagcttccc atagcttccg
     4021 tattgcggca atagatggtg tattgggata gctttttagt aaatcatgga cgagcgctgt
     4081 taaagcaaga gttactggga ctgtaagagt cgggggctga ccttctgtgg tatcagggtt
     4141 tcgcatttta atacccatga cgctggtgat ttgacttgct atcaacgccc gcttaacatt
     4201 actgtctccc tcgagcaact gctcatatcc tgccttagca agccacacat taattccagc
     4261 aaagattttt gctatatcag ttagttcaaa atgattagtc cctaatatga cattatcacc
     4321 acgttccaat gcagt
//
LOCUS       DATNGP010000186         5539 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_1751765, whole genome shotgun sequence.
ACCESSION   DATNGP010000186 DATNGP010000000
VERSION     DATNGP010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5539
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1751765"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1687
                     /gene="thrS"
                     /locus_tag="VN778_04810"
     CDS             <1..1687
                     /gene="thrS"
                     /locus_tag="VN778_04810"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010164333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HXR50318.1"
                     /translation="SEEDFAKIEVEMRKVIEADVPFERFEKPVDEAIAWAVTSKQPYK
                     EELLHDLKRAGTTVAKDLNAEELGTITEGDAQVENVSFYKNGDFTDLCRGPHVESTGK
                     VGAFKLMRVAGAYWRGNEKNPQMQRIYGVAFATEDELNKHLEMLEEAKKRDHRKLGQE
                     LDLFVFSDLVGSGLPLFTPRGTILREEIGKFTQELRERNGFTRVWTPVIAKTDLYKAS
                     GHWDKFGDELFLVKSQETSDEFVLRPMNCPHHVQIYASQPRSYRDLPLKFMENAGDYR
                     DEKTGELHGLSRVRSFTQDDSHAFVADSQIKAVATELVEAAHEMYGVFGMELKFRLSF
                     RDESEGYLGDPQLWEKAQTQIEELAKENNLDYYVEEGEAAFYGPKIDFMATDAIGRQW
                     QVATVQLDFVQPERFGLEYVADDDSKQRPVMIHCALLGSIERFLSVYIEHTAGRFPVW
                     LAPEQIRVITVNQEEKTLVFAGKMERNAADLGLRLGVDNSNESVGKKIRTAELDKVPY
                     TVVVGEKEMASGEVVPRIRRDILVKESDRPIGVEEFLKTVANESKSRVNKTSL"
     gene            1735..2346
                     /locus_tag="VN778_04815"
     CDS             1735..2346
                     /locus_tag="VN778_04815"
                     /inference="COORDINATES: protein motif:HMM:NF018627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HXR50319.1"
                     /translation="MSRETIAVDLDDVLGDENGAIMHFSNKQLGLNHTLEDYTRKAPY
                     ERYWENIWKLGANEGQKHYEAFISSPYKKHLKPIAGAAEALKTLEKERNLVIVTSRRE
                     DMMEYSHEWLEMHFPQVFKEIHFVHLWDEGSQRATKAKICNEIGATYLIDDNLEHCTL
                     AAEAGVQALLFEEYGWNRDRELPKGVVPVIGWAAVTEYFDARD"
     gene            2333..2677
                     /locus_tag="VN778_04820"
     CDS             2333..2677
                     /locus_tag="VN778_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019587557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HXR50320.1"
                     /translation="MHGIEADFRKRVEALVAQIPRGRVMTYGQLAALCGNARAARIVG
                     GVAHFGDPDLPWQRVVNKSGGLASGYPGGRAGHKQVLEGEGVEVSEDYKVNVVELLWW
                     PPGEEPLQAALF"
     gene            2748..3629
                     /gene="miaA"
                     /locus_tag="VN778_04825"
     CDS             2748..3629
                     /gene="miaA"
                     /locus_tag="VN778_04825"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HXR50321.1"
                     /translation="MKKVKPPLVVLVGETASGKSALALEIAQKFEGEIICADSWTVRR
                     EVNIGTAKPSVDEQALVPHHLLDVVGPCEDFTAAVFKDLANQAIEGIVARGKLPILVG
                     GTGLYIDGVVYDFGFLPAGDRGAREELSVLSIAQLLQKIEALGIDVGDVDTRNKRRLI
                     RLIETNGAQPTKKTLRPNTLILGLKVDRENLKERIEKRTDAMLEAGLEDEVRNLQERY
                     GWGCEALKGVGYAQWKGYFEGTQILAETRQKIIKATADLAKRQRTWFRRNNSIHWLHT
                     PVNWTEVVELITTSLDK"
     gene            3667..4290
                     /locus_tag="VN778_04830"
     CDS             3667..4290
                     /locus_tag="VN778_04830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HXR50322.1"
                     /translation="MVEQLFGSKTRVKLLQLFYSNPNRSFYVREITRKIGEQINSVRR
                     ELSNLLSIGIITSDNTNNKLYYEVNQKYEYYEPLQQIFGGGMKKVAAKTSPADTDGDG
                     IVDDDLKAVGHIELAVYTGQFTRDDTAGVDFLIVGEVNPNALQKYVGELEARENKSLR
                     YAVMSLPDFQYRQQIRDRFAVAIAKSKKQVLVDRHNLLQQTEQPEEE"
     gene            4293..4913
                     /locus_tag="VN778_04835"
     CDS             4293..4913
                     /locus_tag="VN778_04835"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXR50323.1"
                     /translation="MDLQFHGANCISVSYKGTRLVTDDNLAELSSKSVVKADDVALFT
                     SRHEGEVPARLVFDGPGEYEVSDISVIGVAARAHMDEDGKSATLFKLIVGDLSVLVTG
                     HIYPELNEAQLEQIGMVDVMIVPVGGNGYTVDPVGALKLIKEVEPKLVIPTHYADKAL
                     NFPMPQQELSNALKELGMEPKETTSKLRLKPSELSDLTQLIVLEKS"
     gene            complement(4914..5123)
                     /gene="rpmB"
                     /locus_tag="VN778_04840"
     CDS             complement(4914..5123)
                     /gene="rpmB"
                     /locus_tag="VN778_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010164343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HXR50324.1"
                     /translation="MQKCDLTGKGKQYGNNVSFSQRHTKKVWKPNLQTKTFMVDGKKV
                     TMKLSTQAIRTLKKKGLLTPVSPSK"
     gene            5204..>5539
                     /locus_tag="VN778_04845"
     CDS             5204..>5539
                     /locus_tag="VN778_04845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HXR50325.1"
                     /translation="MQSNLTASSIVIILVSIVISLVIHEFMHGYVGYLLGDTTAKDEG
                     RLTLNPLNHIDPFMTVILPIITLVTFGAPVLAAKPVPFNPERVKYDEFGAALLAAAGP
                     FANLALAVIA"
BASE COUNT         1448 a         1173 c         1626 g         1292 t
ORIGIN      
        1 ctccgaagaa gattttgcaa agatcgaagt ggagatgcgg aaggttatag aggcggatgt
       61 accatttgag cggtttgaga agccggtgga tgaagcgatt gcttgggcgg taacgagcaa
      121 gcagccgtat aaagaagagc tattacatga cctgaagcga gcgggaacca ctgtcgcaaa
      181 agatttgaat gccgaagagc tcggcaccat taccgaaggt gatgcgcagg ttgagaacgt
      241 gtccttttat aagaatggtg atttcacgga cctctgtcgg gggccgcatg tggagtcaac
      301 gggtaaagtc ggggctttta agttgatgcg cgtggcggga gcttattggc gcggcaatga
      361 aaagaatccg cagatgcagc ggatctatgg cgtggccttt gcgaccgaag atgagcttaa
      421 taaacatctc gagatgcttg aggaagccaa gaagcgagat caccgcaagc tcgggcagga
      481 actggattta tttgtattct cagatctggt aggctcgggg ttaccgttgt ttacgccgcg
      541 gggcacgatt ttgcgcgaag agatcggtaa attcactcag gagttacggg agcggaatgg
      601 ctttacgcgt gtctggacgc cggttatcgc taaaacggac ctttataaag ccagtggtca
      661 ctgggacaag ttcggtgatg agctcttctt agtcaaaagc caggaaacct ccgatgagtt
      721 cgtgctgagg ccgatgaact gcccgcatca cgtgcagatt tatgcatcac aaccccgtag
      781 ctatcgggac ctacccctta aattcatgga aaacgctggc gactaccgcg acgaaaagac
      841 cggggaattg catggactta gtcgggttcg ttcttttacg caggatgata gccatgcctt
      901 cgtagcggat agccagataa aagcggtggc aaccgagttg gtcgaggcgg cacacgaaat
      961 gtatggtgtt tttggcatgg agctgaagtt tcgcctgagc tttagagatg aaagtgaggg
     1021 ctatctcggc gacccgcagc tctgggaaaa ggcccagacg cagatcgagg aactggcaaa
     1081 agagaataat ttagactatt acgttgaaga gggtgaagct gccttttacg gtccaaaaat
     1141 cgactttatg gcaacggatg ctatcggtcg gcaatggcag gtggccacgg tgcagctgga
     1201 ctttgtgcaa ccggaacgtt tcgggctgga gtacgtggcc gatgatgatt cgaagcagcg
     1261 accggtgatg atccactgcg ctctcttggg ctcaatcgag cgtttcttat ctgtttacat
     1321 cgagcacacg gcggggagat tcccggtgtg gctggcgccc gaacagatca gagtaattac
     1381 ggtaaaccag gaagaaaaga cgctcgtgtt cgccggaaaa atggagcgca atgctgccga
     1441 cctaggtttg cgcctcggtg tcgataattc gaatgaatcc gtcggcaaga agattcgcac
     1501 cgccgagcta gacaaagtcc cctacaccgt cgtggtgggc gaaaaggaaa tggcaagcgg
     1561 ggaagtggta cctcgcattc gccgggatat tctggtcaaa gagagcgatc gccctatcgg
     1621 tgtcgaggaa ttcctcaaaa ccgtggccaa cgaatccaag agtcgcgtta ataaaacttc
     1681 tctgtagaat aacgctcaag gtttactatt gcctggttgt tataatgctt tcctatgagt
     1741 agagaaacta tagccgtaga tctggatgac gtacttggcg atgaaaacgg tgccattatg
     1801 cacttcagta ataaacagtt gggtttaaat cacaccctcg aagactatac tcgaaaagct
     1861 ccctacgaga gatattggga gaatatttgg aaattagggg caaatgaagg acagaagcat
     1921 tatgaagctt ttatttcgtc gccctataag aagcacctga aacctatagc tggtgcagca
     1981 gaggctctaa aaactttgga gaaagagcgg aatttagtaa tagtgacctc tcgcagagaa
     2041 gatatgatgg aatatagcca tgaatggctt gagatgcact ttccgcaagt cttcaaagag
     2101 attcattttg tccatctgtg ggatgaggga agtcaaaggg caactaaggc taagatctgt
     2161 aatgagatcg gtgctactta tcttatagac gacaacttag aacactgcac gcttgcagca
     2221 gaggcaggag ttcaggcatt actttttgaa gaatacggct ggaataggga cagagaactg
     2281 cccaagggag tcgttccggt tataggctgg gctgctgtaa cggaatattt tgatgcacgg
     2341 gattgaggct gattttcgga agcgagtgga ggcgctggta gcgcagattc cgcggggcag
     2401 ggtgatgact tatgggcagc tcgcggcgtt gtgcggtaat gcgcgggcgg cacggattgt
     2461 tgggggagtt gcgcattttg gcgatcctga tctgccgtgg cagagggtag ttaataagag
     2521 tggtgggctg gcgtcgggct atccgggtgg tcgggcgggg cacaagcagg tgttggaagg
     2581 cgaaggggtc gaggtgagcg aagattataa agttaatgtc gtggagcttt tatggtggcc
     2641 gccgggcgag gagccgctgc aagctgcatt attttaggtt ataatttgtg gccatacaga
     2701 atctgtacag aaaatgtaca gaaaagatat ggtgtaaagt gagagtgatg aaaaaagtaa
     2761 agcccccgct agtggttttg gttggtgaaa cggcgtcggg gaagtcggcg ctggcgctgg
     2821 agattgcaca gaagttcgag ggggagatta tttgtgcgga tagctggaca gtcagacggg
     2881 aggtgaatat cgggacggcg aaaccgtcgg ttgatgagca ggcgctggtg ccgcatcatt
     2941 tgctggatgt agtggggccg tgcgaagatt ttacggcggc ggtgtttaag gatttagcga
     3001 atcaggctat tgaaggtatt gtagcgcgag ggaaactgcc catcttggtc ggtggtacgg
     3061 gactctatat cgacggggta gtttatgatt ttggtttttt gccggcaggc gacaggggtg
     3121 cacgcgagga gctgagtgtg ttgagtattg cacaactact gcaaaaaatt gaggctttag
     3181 gcattgatgt aggggatgtc gatacacgaa acaagcgccg gctaatacgc ttaattgaaa
     3241 cgaatggcgc ccagccgacg aaaaaaacgc ttcggccaaa cacattgatt ttggggctga
     3301 aagtcgatcg tgagaaccta aaagagcgca ttgaaaagcg cacagatgcg atgctcgaag
     3361 ctggcttaga agacgaagta aggaatttac aggagcgtta tggttggggc tgtgaagcac
     3421 tcaaaggtgt ggggtacgcc cagtggaagg ggtactttga aggtactcaa attctggccg
     3481 aaacccggca aaaaatcatc aaagcgacgg cggatttggc taagcgccag cgcacctggt
     3541 ttcggcgaaa taatagtatt cattggctac acacccctgt taattggact gaagtcgtag
     3601 aactaataac aacaagtttg gacaaataag atttcattcg cgctctatct ttgctacaat
     3661 aaaaacatgg ttgagcaact ctttggatca aaaacccggg tcaaattatt gcaattgttc
     3721 tatagcaatc cgaaccgctc attttacgtg cgggaaatta cgcgcaaaat cggcgaacaa
     3781 atcaactcgg tgcgccgtga gctcagcaac ttgctgagca tcggcatcat tacgtccgac
     3841 aacactaaca acaagctgta ctacgaagtc aatcaaaagt atgaatatta cgagccgcta
     3901 cagcaaattt ttggcggcgg catgaaaaag gtagccgcta agaccagccc agccgatacc
     3961 gacggtgacg gcatcgttga cgatgaccta aaagctgtcg ggcacatcga gttagcggtc
     4021 tacaccgggc agttcacccg tgacgacacg gctggcgtcg acttcctaat cgtcggcgaa
     4081 gttaacccaa atgccttaca gaagtatgtc ggcgagttgg aagcccgcga aaacaagagt
     4141 ctacgctacg ccgtcatgag tttgcccgat tttcagtatc gtcaacagat tagggaccgc
     4201 ttcgccgtgg cgattgccaa atccaagaag caagtgctag tcgatcggca taatctgttg
     4261 cagcaaactg agcaacccga agaggagtaa gcatggacct gcaatttcat ggtgctaatt
     4321 gtattagtgt gagttataag ggtacgcgcc tagtaactga cgataatttg gccgagctaa
     4381 gtagcaaaag tgtggtgaag gccgatgatg tggcgctctt cactagtcgc catgaaggtg
     4441 aagtgcccgc ccggttagtt tttgacggtc caggcgagta tgaagtgtcg gatatctctg
     4501 ttatcggggt ggcggcccgg gctcatatgg atgaggacgg caaaagtgcc acgctgttca
     4561 agctgatcgt tggcgacttg agcgtgctag tcaccgggca tatttatccg gagctcaacg
     4621 aagcacagct agaacagatc ggtatggtcg acgtcatgat cgtgccggta ggtggcaatg
     4681 gctacactgt cgatccagtg ggggcgctga agcttatcaa ggaagttgag ccgaaactag
     4741 tcattcccac acactatgcc gataaagcgt tgaatttccc catgccccag caagagctaa
     4801 gcaatgcttt gaaagagctg ggtatggagc cgaaggaaac gaccagcaaa ctccggctca
     4861 agcccagcga actgagcgat ctgacgcaac tgattgtact agaaaaatcc taattattta
     4921 gagggcgaaa caggggtcag caggcccttc ttcttgaggg tgcgaatagc ctgagtgctg
     4981 agcttcatgg tgactttttt gccgtcgacc ataaacgtct tggtctggag attaggcttc
     5041 cagactttct tggtatggcg ctgagaaaag ctaacgttat tgccgtattg cttgcctttg
     5101 ccggtgagat cacatttttg catagaaatt ccttagattc aaggtgttat tgtagcggaa
     5161 ttaccgatgt aagtcaaccg tctccgaatg atatactgaa ggaatgcaat ccaacctgac
     5221 ggccagcagt atcgttatta ttcttgtctc gatcgttata tcactcgtca ttcacgagtt
     5281 tatgcacggg tacgtcggct atttgcttgg tgatactacg gccaaagatg aggggcggct
     5341 gaccttgaac ccgctcaacc acatcgaccc gttcatgacg gtaatcttgc cgatcatcac
     5401 tttggtgact ttcggagcgc cggtgttggc ggccaagccg gtgccgttta accccgagcg
     5461 agtgaagtac gacgagttcg gcgccgcgct actggccgcg gcgggaccgt ttgccaatct
     5521 ggcgctagca gttatcgca
//
LOCUS       DATNGP010000187        12732 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_800306, whole genome shotgun sequence.
ACCESSION   DATNGP010000187 DATNGP010000000
VERSION     DATNGP010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 12732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12732
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_800306"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..393)
                     /locus_tag="VN778_04850"
     CDS             complement(<1..393)
                     /locus_tag="VN778_04850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50326.1"
                     /translation="MKASNKVQVAQARGYLRARLATGQYIAQVNNRLASNKQLVTIES
                     HKTNTFNINLQALLAPEAVLPYGATSVITDASSIYYVNLQNHSLYTLSSAGVPMVVST
                     TSFDHIVWENTHYGIGIDYSQSTLYEIRN"
     gene            complement(566..2752)
                     /locus_tag="VN778_04855"
     CDS             complement(566..2752)
                     /locus_tag="VN778_04855"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tip lysozyme"
                     /protein_id="HXR50327.1"
                     /translation="MLILGQVAAVNALPPGQQEIFDEGINYFDLTTTCDTSGDTPSTP
                     SSTNGASSLTALTGDQKISGTFMLGFDAGTPKPTIESVFKQYKPGGIFVLGTKDAAAA
                     GFNKAFFEQLAQDAGHKIVAASDEEGGQIHRYGYGFSLPSPHDMSSMSDADVQSLGKQ
                     VGQAMASSGLNTDLAPVLDVSTDSSGTDTGVTGRAFSNDPGVVAAKGGAFATGLQSAG
                     INPVYKHFPGLGSSSGNTDNQPVTSPSLSQLESHDLKPYESIVNKNGAAVMLDNAHIP
                     GLTASGEVASTSAPAINLLRNKYNFNGLIMTDDLTATGVQLPLASAVAKALQAGVDMP
                     IFTYSSNGQLDAAISAAKAAGINTDKALQNIDNFLAHTANTGGSTQMNSCCGGGSTTL
                     VGNTNAEKVWNFFIGKGLTNVQVAAIMGNLQQESGFSPTIVNSGSGAYGLGQWLGGRL
                     TALKGQPNYTDLGTQLDFMWSELNGSYKSSVLDPLKQATDLKTAVNIWLIHYEAPCTE
                     GSSACTIELTKRFGFAIAWINHANGGNGSGSAATSSETSDSCSSGSSGSGNYTNPFHD
                     MSNLGASRIDEGVDYYAKSGSVPIYAIGNGKVTLATDHSSFYTTSGGHADWITYQLTD
                     GPATGKYIYVAEACPPSVHTGDVVTSNTVLCNVLPDSIETGWASDATTQIAAAHDVYR
                     EGYETAYGVNFNQLLEKLGAPGGHLDTSHDPGGTVLGSLPANWPQW"
     gene            complement(2806..5709)
                     /locus_tag="VN778_04860"
     CDS             complement(2806..5709)
                     /locus_tag="VN778_04860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50328.1"
                     /translation="MNPEHKVYDDHDEEPVTRPDLRALEGGGETSEPKQGHLSAVPDE
                     ESSTESPKSLFNAEGDTASSSKGSGRSVSPHDLSQAESTPLMGAASAATGGITAKAGA
                     IKNLFWGTSSRRKKTIFGGGAIGLIAGGIFGLTILSGPAQLIQLSAVLQHPLAGMSQA
                     SSDRLGKLTRYAGARDLGETRMGTLGRQILRPAVQQLSDAGIDFTPDALGTAKATIDI
                     GKLKSKFPGLENMSTDEQRSFLSEQFDGITPEQFQDIGGGKFEVNTGRFNSAALSALR
                     DNALHLLPDGTKLNFIENRVLAKYLGINSLFHPLKKLTTDQLKKLTTPAEAKAEEENR
                     ETTNIDKPLETTEGTPSIRERLKGEVDPGAVDSIGKGLMVAAVVCTIHNAADDAVALN
                     HAAIVMPAEAKTADLVAVGEQVKSGQDTTIQQVGAVEQGLTDDKGQTVWQGKALQALE
                     GNPTPTGSDLPTEYAQAFSGKTTADSIKKTTNIEVLGINACSGVGEVAQVVAGLALLA
                     LAIPTDGSSFAAWSALKAGETAGTMGVFSFFQHELGNLIKDKAIVPAVLSGPLGGNIM
                     AYGARAAANTAAMASGGIQLAGSAAQAFTYQQQQQDKQQFRSESFFARTFDINDYRSL
                     AGRLADSITPSFSRNMTNFASVFANFGSRIPKTLSALLPHASAANQPYDWGFPLVGIP
                     NSLLSDPALSNPYENATTVQALLQNNGDYMTRATSCFGVTIDSSTADITVADHEADPN
                     SQAYTDSNCSDTKDPNWRRVIMFVFDTRTMQAAACYTGDDQSCSEIGYDTAGSSSSSG
                     GAGGGTSSSSSCSDGSGNYSALVSSGASFAGTDQGIDFVPSGSGGFDICAPASGTITL
                     ADQAGHHFDRTPGQAEIIEKLDQNPNAPDSSQYIYYAEIIQINNSIKVGARISAGDVI
                     GHNAQSPGIEVGWGQDPVHGFLCSLGATVGGPTPCGRSFNDWIQGLTTGKVPS"
     gene            complement(5779..7710)
                     /locus_tag="VN778_04865"
     CDS             complement(5779..7710)
                     /locus_tag="VN778_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008578486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraC"
                     /protein_id="HXR50329.1"
                     /translation="MFGKKNPPVDPVAVAQAQRAREEQEVQAAFQKGVTALRDFIAPS
                     SLEFSSTHFQLGTRYARTYYVYGYPRQLYTGWLSGMVNLDEVMDMSLYVYPVESQVVL
                     ENLKKKVTQLEAGIQLDSEKGRVRDPAKQAQILDAEEMRDKLQVGEERFFRFGFYFTI
                     YAGSMEELEFVSHKVESMLGQQLVYSKPASSQQEQGFNSTVPQLVDQLQIRRNMSTGA
                     LSTSFPFTSADLTQDNGILYGINMHNSGLVIFDRFSLENGNSVVFAKSGAGKSFAVKL
                     EALRSMMFGTEIFIIDPENEYQRMCDAVGGAYVRLSLNSSVRVNPFDLPKVVDSEEAD
                     NALRSNLITLHGLLRLMMGGAQTQMLGGNAAYLPALAPSEEADLDAALIATYAKAGIT
                     NDPLTHTSIPPTITDLYDTLLHMGGTGPQLAQRLRKYTSGTFAGIFSQQSNIDINNPL
                     VVFNVRDLEDELRPVAMYIVLNYIWNKTKTDQRRRILIVDEAWQLMKFEDSANFLFSL
                     AKRARKYNLGITTITQDVEDFMGSRMGRAIVANASMQFLLKQSPSAVDVLSDVFKLTS
                     EERKRLSQFPVGQGLFFAGQSHVHVQVVASPTETGLITTNPAQVQQQAVTEQSGELNQ
                     PAGTIDLGNRLSPGSAGPV"
     gene            complement(7710..8771)
                     /locus_tag="VN778_04870"
     CDS             complement(7710..8771)
                     /locus_tag="VN778_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008578487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50330.1"
                     /translation="MNPNQPNQAATPGQPAPLPEIQPIGLPREESQRVVPAMVAAEQV
                     AQVAQQVPATTMPMPTLAPPVPIGGTMPQPPASRQPIPPPSAVAPVAPAAPQPVTSGA
                     PATPPPAPKTNPNSTQNTLQMAEVRDGIVIMNDGSFRSVVMVKSINFDLMSPQEQEAV
                     EYSYQGFLNSLYFPIQIFTRSQRVDLQPYIEKLDKIRTEHDNMLLALLMEDYIGYIDA
                     LSQHTNIMDKKFYVVIPFFPVVDAQKALSQSKNFLSGLGGLFNSKEQHVVINEADLNN
                     AKTELRNRVQAVLAGLQQCGIQGLPLDTQELIELYYDTYNPDTATRQQLKNFNDLTAD
                     VITKGQGMAPQAHLKRELQ"
     gene            complement(8787..9887)
                     /locus_tag="VN778_04875"
     CDS             complement(8787..9887)
                     /locus_tag="VN778_04875"
                     /inference="COORDINATES: protein motif:HMM:NF024078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HXR50331.1"
                     /translation="MATYKLMQDIEAEDHILGPLSLRQFIFALVAVFCGYMCFIGVAK
                     HFFVLWLIFMWPLLFFGFFAFPWGRDQPTEIWALARLRFLFKPRQRIWDQSGVKELVT
                     ITVPKRVEPVLTDGLSQTEVDSRLRALANTLDSRGWAVKGVDLNAYSNPDPLATISTD
                     RLITMDSMPHQVPSYDVHPSDDMLDEASNPVAQQFDQMIARSEATHRQALITQMNDTS
                     PAPVRSQQPMANAPPTDYWFMSPQASTAPRPGKAAFAPAQVQLPGSDSVVASVPSSLD
                     EEALASQLKANNSSKQVANTHLRTIQPLGSHPPQPPVASPVPPPSPAPPDPAILSLAN
                     NNDLNVATLAREARKAKHDEPPQSDEVVISLH"
     gene            10087..10848
                     /locus_tag="VN778_04880"
     CDS             10087..10848
                     /locus_tag="VN778_04880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50332.1"
                     /translation="MSEFFNKTNEPDYWETAANQHVNRAKTARREVMVHAGELHRAAQ
                     ELAGRLTDRGVPPNITVVATEVEKAGRARTAWRKQFRWQEDPHHYPVGAQVLGRGWLI
                     SQTSVRSGKANSRSITLLEDGSLVISGPTTGEGSARQAEAYREPAPGLESVPGKTILG
                     RNKNIHGTEKRKWQEAPRGAVRAAIDLTTFPTHPLFATEKSYANTTLGMPDLGANPDS
                     AVDPHTPEAYVAANLERLEHDLRSTGRELLGYDRP"
     gene            complement(10897..>12732)
                     /locus_tag="VN778_04885"
     CDS             complement(10897..>12732)
                     /locus_tag="VN778_04885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50333.1"
                     /translation="ILPRLLIAAVLINLSIYLVALAVDITNVVGNGVEALIEGPFKNA
                     GAFKLHIGGAAGDVGMGALLGSAGLIWKLSLAPALLEFLAFVVVLPAFLTFIAILVTV
                     ILRRGLILFLILISPVAFALYCLPNTEQYFRKWWDLLLRTLIVYPIIAAAFAIGNVLS
                     VTMSSTSSGLIDGTLAQLLGIIALFVPLLLIPYSFRLAGGFLGRAHDAVTSVRDRAHK
                     ATEGRRQRAQSHLTRASIQGRQNWYSTLQDSASRGGAFRRRGLGGLARAVGGYNIEAR
                     SSAANAEIGKEFNDIIATGKDDEIGGLSVDKRYAQSRTTSDGRKQFKSLGGAWINEAD
                     VDAGHRRWGGNAYAMQASLAYEMRKADTEQDVQRIASDYHNVAQANGLTDQQAAGAWI
                     GAAFQNQDKHLQFKYTNWQTGQMDADQRESFMSEIYEKRGSYNMAQMHAGTIGQVIQA
                     NRDAQAVIANSPTGLTGQARQDEIDQAKRMQGQAASIAESFVSRWGGGSGIGGEGAEL
                     AATMQQQAAAAAAVQQQVAQGPATGTQQAGPQIPASVARGAVNLGQPRAAGGLGPTVQ
                     TNTPGAAHVAERVRQLAVETGVYQTLDPSSDTRPSQGPPPISRQN"
BASE COUNT         2917 a         3356 c         3389 g         3070 t
ORIGIN      
        1 atttcggatt tcatacaaag tagattggct gtaatcaata cctatgccat aatgcgtatt
       61 ttcccaaaca atatgatcaa acgaagttgt gcttactacc atcggtacgc cggcgctgct
      121 gagggtataa agtgagtggt tttgtagatt gacataatag atactagagg cgtctgttat
      181 aacactagtt gctccgtatg gtaaaacggc ttccggagcg agcagggcct gaagattgat
      241 attgaaagta ttagttttgt gggactcgat ggtgactaat tgtttattag aagccaagcg
      301 attattaacc tgggcaatat attgtccggt agccaagcgg gctcgcagat aacctcgagc
      361 ttgggctacc tgaaccttat tgctcgcttt caatatctca atattattgc tcggattgtt
      421 tgtggttacg acaattgtgc cggtggttaa atagcgttgg gtgaataaga gaagaatgag
      481 cgataaaacg accaaaaagc aagcgaaggc taccttcctt ggatggtttt ggagatatag
      541 tacaaacctt tccctcataa ttcacctacc attgcggcca atttgctggc agcgagccca
      601 acacggtgcc gccggggtca tgtgatgtat ccaaatggcc tcctggcgcg ccaagtttct
      661 ccagcaactg attaaagttt acaccataag cagtttcgta gccttcccta tatacatcat
      721 gcgcagccgc tatctgggtt gtggcatcag aagcccaacc agtctcaatg ctgtcgggca
      781 acacattaca taaaacagta tttgacgtta cgacatcacc ggtatgtact gagggtgggc
      841 aggcctcagc aacatatata tatttgcctg tggcgggccc gtcggtgagt tggtaagtga
      901 tccaatcagc atggccgcca cttgtggtat aaaagctgct atggtcggtt gcaagagtga
      961 ccttgccgtt ccctatcgca tatattggta ctgaacccga ctttgcgtaa taatccacgc
     1021 cttcatcaat tcgactagca cctagattac tcatatcatg gaaaggattg gtatagttgc
     1081 ccgaaccact actaccactt gaacaactgt cggaggtctc cgacgaagtg gcagcgctac
     1141 ccgacccatt gcccccattt gcatggttta tccaggcgat tgcaaaacca aaacgctttg
     1201 taagctctat tgtgcaggcg gaagaaccct cagtgcacgg cgcttcgtaa tgaatcagcc
     1261 aaatattaac tgccgtcttt aagtccgtcg cctgctttaa ggggtctaat acagaagact
     1321 tatagctgcc gttcagctcg ctccacatga aatcgagttg agtgcccaaa tcggtataat
     1381 tgggctggcc cttgagtgcg gttaagcggc cacccagcca ctgtccgaga ccataagccc
     1441 cactgcccga attaacaatg gttgggctga acccggattc ttgttgcaaa tttcccataa
     1501 tcgctgccac ctgtacattg gtaaggcctt tgcctatgaa gaagttccag actttttcag
     1561 cattcgtgtt tccaactaaa gtagttgagc caccgccgca gcaactattc atctgggtag
     1621 agccgccagt gttggcggta tgcgcgagaa agttatcgat attttgcaag gccttgtcgg
     1681 tgttaatgcc ggcggctttt gcggcgctaa tagccgcatc taattggccg ttgctactat
     1741 acgtgaatat tggcatatcg acgccggctt gcagagcttt ggcgaccgct gacgcaagcg
     1801 gcagttgtac accggtagcg gtaaggtcat ccgtcataat caggccattg aaattatatt
     1861 tattacgaag caagtttatg gcaggcgcgc tggtgctggc gacttcccca ctggcagtta
     1921 gcccgggtat gtgcgcgtta tctaacataa ctgcggctcc atttttattc acaatggatt
     1981 cataaggctt gagatcgtga ctttccagtt ggctcaacga aggcgaggtt acaggctggt
     2041 tgtctgtgtt tcctgaagag ctgccgagcc cgggaaaatg cttatagaca gggtttattc
     2101 cggccgactg caggccggtc gcaaaggcgc cacccttagc tgccaccacc ccaggatcat
     2161 tagagaaggc tcggccggtt acccctgtat cagtaccact tgagtcagtg gatacatcga
     2221 ggactggagc caggtcggta ttcagtccgc tggaggccat ggcttgtccc acttgctttc
     2281 ccaagctttg cacgtctgca tcgctcatag agctcatgtc atgcggtgac gggagactaa
     2341 acccatagcc atagcggtga atttgtccgc cctcttcgtc gctcgcagcc acaattttat
     2401 gacctgcatc ctgtgctagc tgttcgaaaa aggctttatt gaagccagcc gctgccgcgt
     2461 ccttggtgcc aagtacaaaa ataccccctg gcttatattg cttgaaaaca ctctcgatgg
     2521 taggtttcgg agtgccagca tcgaatccaa gcataaaggt acccgatatt ttttgatcac
     2581 cagtaagggc cgttaatgag cttgcaccat tcgtgcttga tggtgtggac ggtgtgtcgc
     2641 cagaagtgtc gcaggtggtg gtgaggtcaa agtaattaat tccctcatca aatatctcct
     2701 gctggccggg gggcaacgca ttaacggctg caacctgtcc gagtataagc aaaataatag
     2761 ataatgaaca gagtatcctg tggcgtaaaa tgcggcgctt gcgtatcatg acggtacctt
     2821 acctgtggta agaccttgta tccagtcatt gaaacttcgg ccacacggag ttggtccgcc
     2881 taccgtagct ccaaggctgc acaggaatcc atggactgga tcttgccccc atccaacctc
     2941 aatccctgga ctttgggcat tatggccgat gacatcgcca gcactaatgc gcgcgcctac
     3001 cttaatgcta ttgttaattt gaatgatctc ggcatagtaa atatactgac tgctatccgg
     3061 ggcattcggg ttttgatcca atttttcgat aatttcagct tgcccaggtg ttcggtcgaa
     3121 atggtgtccg gcttggtcgg ctagtgtaat tgttccggaa gctggcgcgc agatgtcaaa
     3181 tcctccactg cccgagggca cgaaatcaat tccttggtca gttcccgcaa aacttgctcc
     3241 cgaacttacc aaggcagaat aatttccact gccatcactg cagctactcg acgatgacgt
     3301 gccgcccccg gcgcccccag aagaagatga gctgccagct gtatcgtaac caatctcaga
     3361 acaggactgg tcgtcgcccg tatagcaggc agccgcctgc atcgtgcgag tgtcaaagac
     3421 gaacataatc acccgccgcc aattcgggtc tttagtgtcg ctacagttac tgtcagtgta
     3481 agcttgacta tttggatcgg cctcgtgatc ggctaccgta atatcggctg tgctggagtc
     3541 gatggttaca ccgaagcaac tggtggcccg ggtcatgtag tcaccattgt tttgtaataa
     3601 agcctgcaca gttgtggcgt tttcgtaagg gttgcttaga gccggatcac ttaagaggct
     3661 attcggaatt ccaaccaggg gaaagcccca atcgtatggc tggttggcgg cgctagcgtg
     3721 gggcagcaaa gctgataaag ttttcggaat tcggctgccg aagttggcaa aaacgctagc
     3781 aaagttggtc atattgcgcg agaagcttgg cgtgatggag tctgctagcc gtcctgccaa
     3841 tgaccggtag tcatttatat caaaggttct agcaaagaag ctctcggacc tgaactgctg
     3901 tttatcctgc tgctgttgct ggtaagtgaa tgcctgagca gccgaaccgg ccaattgtat
     3961 gcccccggaa gccatggcgg ctgtattggc tgccgccctg gctccataag ccatgatatt
     4021 gccccccagt ggaccgctga gcaccgccgg cacaatagcc ttatctttta ttaaattgcc
     4081 gagctcatgc tggaagaaag aaaagacacc cattgttcct gccgtctcgc ccgcttttag
     4141 tgctgaccat gcagcaaaag acgacccatc tgtcgggatt gcaagtgcta acaatgctaa
     4201 gcctgccaca acttgagcta cttccccaac gccactacaa gcattgatgc ccaacacctc
     4261 tatattagtt gtttttttaa tactatctgc ggttgttttt ccggaaaaag cttgcgcata
     4321 ctcggtgggc aaatcggatc cggtgggggt tgggttgcct tcgagtgcct gaagcgcttt
     4381 tccttgccac acggtctggc ctttgtcgtc tgtcaggcct tgctcaactg ctcctacctg
     4441 ctggatagta gtatcttggc cggacttaac ttgttcaccg accgcaacca ggtcagcagt
     4501 tttagcttcc gcgggcataa caatggctgc atgattaagg gctaccgcat catcagcggc
     4561 attatggatg gtgcaaacca cagcggcaac cataagtcct ttgccaatac tgtctactgc
     4621 accaggatct acctcgccct ttaaccgctc ccttatagaa ggggtgcctt ctgttgtctc
     4681 caggggctta tcaatgttag tagtttcccg gttttcttct tcggctttag cttccgctgg
     4741 cgtcgtcaat tttttcaact gatcagtagt caattttttc agcggatgaa acaggctatt
     4801 aattcccaag tatttagcca gcaccctatt ttctatgaaa ttgagcttgg taccatctgg
     4861 caggaggtgg agggcattat cccgaagagc gcttaaagca gcggagttaa atctgccggt
     4921 attgacttca aacttgccgc cgccgatatc ttgaaactgt tccggagtta tgccatcgaa
     4981 ttgttctgat aaaaagcttc tttgctcatc tgtcgacatg ttttccaagc cgggaaactt
     5041 actcttcagc tttcctatgt caatagtggc tttcgcagtt cccaaggcgt ccggggtaaa
     5101 gtcaatcccc gcgtcgctta attgctgcac agcgggacga agaatctgcc tccctagtgt
     5161 acccattcgt gtttcaccga gatcacgggc cccggcgtat cgtgtaagct tgccaagccg
     5221 atctgacgaa gcctgactca tgccagccaa ggggtgctgt aggacggcgg agagctgtat
     5281 caattgtgca ggtccggaaa gaatggtaag accgaagatg ccacctgcaa tcaggccgat
     5341 agcaccacca ccaaagatag tttttttgcg acggcttgag gtcccccaga aaagattctt
     5401 tatagcgcct gcctttgcgg tgattccgcc agttgcggct gacgcagctc ccatcaaagg
     5461 ggttgactcg gcttgactca aatcgtgtgg actgacggat cgcccagagc ctttactaga
     5521 tgatgccgtg tcaccttcgg cgttgaaaag gctcttaggt gactcggtgc tgctttcctc
     5581 gtccggtacc gcgctcaaat gcccctgttt cggctcgcta gtctccccgc cgccttccag
     5641 ggcacgcaaa tcaggccgag tgaccggctc ctcatcatgg tcatcataga ccttgtgttc
     5701 aggattcatg tttgtgttga cctacactta agtccttatg gttttatttt acaacttaac
     5761 ccgaggaatt actactgttt acacggggcc ggcgctgccc ggcgacaacc ggttgccgag
     5821 atcaatcgtc ccggccggct ggttgagctc gccggattgt tcagtgacag cttgttgctg
     5881 gacctgcgcg gggttggtgg tgatcaggcc ggtctcagta gggctggcga ctacttggac
     5941 gtgaacgtga ctttggccgg caaaaaacag gccctggccg accgggaact ggctgagacg
     6001 cttgcgctcc tcgctggtaa gcttgaagac atcactcagc acgtcgactg ccgacggcga
     6061 ctgcttcaac aggaactgca tcgaagcgtt ggcgacaatc gcccggccca tgcgcgagcc
     6121 cataaagtcc tcgacgtcct gggtgatcgt ggtaatgccg agattgtact tgcgagctcg
     6181 cttggccaaa gagaagagga agttagccga atcctcgaac ttcatcagtt gccaggcctc
     6241 atcaacgatc aagatacgac gccgttgatc agttttcgtc ttattccaga tgtaatttag
     6301 cactatgtac atagctacag gacgcagctc gtcctccagg tcacggacat tgaagaccac
     6361 cagcgggtta ttgatgtcga tgttgctctg ttggctgaaa atgccagcga acgtacccga
     6421 agtgtacttg cgcagccgct gagccagctg cgggccggtg ccgcccatgt gcagcaaggt
     6481 gtcgtacagg tcggtgatag tcggcgggat ggaggtgtgc gtcaaaggat cattggtgat
     6541 gcctgctttg gcgtaggtcg cgatcagcgc ggcgtcgaga tctgcctcct cagaaggggc
     6601 cagcgccggc agataggcgg cattgccgcc gagcatttgg gtttgcgcgc cacccatcat
     6661 caggcgtagc aggccgtgca aagtaatgag attagagcgc agagcgttgt cagcctcttc
     6721 gctatcgacg actttcggca ggtcgaatgg gttaacgcga acagaggagt ttaggctcaa
     6781 acgcacgtag gcaccgccca ccgcatcaca catgcgctgg tattcgtttt cgggatcgat
     6841 gataaagatt tcggtaccga acatcatgct gcgcaaggct tccagcttca ccgcaaagct
     6901 tttgccggcg ccggacttcg cgaagaccac cgaattgccg ttttccagag agaagcggtc
     6961 gaaaatcacc aggcccgagt tgtgcatgtt gataccgtag aggatgccgt tatcctgggt
     7021 cagatcggcg ctggtaaacg gaaaactggt gctgagcgcg cctgtgctca tattgcgccg
     7081 gatctgcagc tgatccacta gctgcggcac cgtgctatta aagccctgct cctgctgcga
     7141 actagccggc ttcgaataca ccaactgctg cccgagcatc gattccacct tgtgactgac
     7201 aaactccagt tcttccatac tgccggcata aatcgtgaag tagaagccga agcggaagaa
     7261 acgctcctcg cccacctgca gcttatcgcg catttcttcg gcatccagga tctgggcttg
     7321 cttggccggg tcgcgtaccc tgcctttctc gctgtcgagc tgaataccag cctccagctg
     7381 cgtgaccttt ttcttcaagt tttccagcac cacctggctc tcgaccggat acacgtaaag
     7441 gctcatatcc ataacttcgt cgaggttgac catgccgctc agccagcccg tgtagagttg
     7501 tcgcggatag ccatagacgt agtaggtccg ggcataacgg gtgccgagct ggaaatgcgt
     7561 gctgctaaac tccaatgagc ttggcgcgat gaagtcccgc agcgccgtga cccccttctg
     7621 aaaggccgcc tgcacctctt gttcctctcg agctcgctgc gcttgagcga ccgcgacggg
     7681 atcaactggt gggtttttct tgccaaacat tactgtagct cccgcttcag gtgcgcctgt
     7741 ggtgccatgc cctgtccctt cgtgataaca tccgctgtca gatcgttgaa atttttgagc
     7801 tgctgacggg tggcggtgtc cggattgtag gtgtcatagt acagctcgat cagctcttgc
     7861 gtatcgagtg gcaagccctg gataccgcac tgctgcaaac cggccagcac ggcctgcacc
     7921 cggttgcgta gctcagtttt cgcgttattg aggtcggctt cattgatgac gacatgctgc
     7981 tctttggaat tgaacagccc gccgagaccg ctcaggaaat tcttgctctg actcagcgcc
     8041 ttttgagcgt caacgaccgg gaaaaacggt atgactacat aaaacttctt gtccatgatg
     8101 ttggtatgct ggctgagagc atctatataa ccgatgtagt cttccatgag caaggccaat
     8161 agcatgttgt cgtgctcagt acgaatttta tcgagctttt cgatgtaagg ttgcaggtcg
     8221 acccgctgag accgcgtaaa aatctggatt ggaaaataca aggagttcaa aaacccctgg
     8281 tagctgtact ccactgcctc ttgctcctgc gggctcatca ggtcaaagtt aatactcttg
     8341 accatgacca ccgagcggaa cgagccatcg ttcatgatga cgatgccgtc gcggacctca
     8401 gccatctgca gggtgttttg cgtgctattc ggattggttt tgggggccgg aggtggtgtg
     8461 gcaggggcgc cagaggtaac gggctgtggt gccgccggag caaccggtgc tacggccgat
     8521 ggtggcggga ttggctgacg gctggccggc ggctgcggca tggtgccgcc aattggcacg
     8581 gggggtgcca gcgtcggcat aggcatcgtc gtggccggga cttgctgggc cacttgcgcc
     8641 acttgttcgg cggccaccat agctggtacg acccgctgcg actcctccct cggtaaacca
     8701 ataggctgaa tctccggcag cggtgctggt tgacccggcg tagcggcttg atttggttga
     8761 tttgggttca tctatcagtg cccaccttag tgcaacgaaa taacaacttc atcactttgt
     8821 ggcggttcat cgtgcttagc tttacgggct tcgcgggcca aagttgccac gttgagatcg
     8881 ttgttatttg ctagagatag tatagcaggg tcaggcggcg caggactagg cgggggcacc
     8941 ggactggcta ctggcggctg aggcggatga ctgccgagcg gctgaatggt tcgcaggtgg
     9001 gtattagcga cctgcttgga actgttgttg gccttcagtt gcgaggcgag cgcttcctcg
     9061 tcgagcgacg acggtaccga agccaccaca ctatcgctac ccggcagttg cacctgagcc
     9121 ggcgcaaaag ccgctttgcc aggtcgcggc gcggtgctgg cctgcgggct catgaaccag
     9181 taatctgttg gcggcgcatt tgccatgggc tgctgactcc tgacaggcgc cggcgaagtg
     9241 tcattcattt gcgtgatgag ggcttggcgg tgcgtcgctt cggacctcgc aatcatctga
     9301 tcaaactgtt gtgctacggg gttgctagct tcatcgagca tgtcatcaga cggatgcaca
     9361 tcatagctgg gcacttgatg cggcatgcta tccatagtga tcaacctgtc cgtgctaatc
     9421 gtagctaacg gatcagggtt gctataagca ttgagatcga cgcccttaac cgcccaaccg
     9481 cggctatcca gcgtatttgc cagcgcccgc aagcggctat cgacctcagt ttgactcagg
     9541 ccatcagtca gcaccggctc aacccgcttc ggcaccgtaa tagtcactag ctccttgacc
     9601 ccactctgat cccaaatccg ctgccgcggc ttaaacaaaa agcgtaagcg cgccagtgcc
     9661 cagatttccg ttggctgatc ccttccccac ggaaaggcaa agaagccgaa aaacaacagc
     9721 ggccacataa aaatcaacca caagacgaaa aaatgcttcg ccacgccgat aaaacacatg
     9781 taaccgcaaa acaccgccac cagcgcaaaa ataaactggc ggagactaag cggcccgaga
     9841 atatgatcct cagcctcaat gtcctgcatg agtttgtatg ttgccataaa ctaagcttaa
     9901 gtataacaac tttatcttgt gcccgctacg acaacatcgt gaacgggaaa attgacaata
     9961 cggcaattat ttgctattat agtattcaag agttaaatta aaaaaatgtt caaaaaaatc
    10021 accaaatacg aaccatttac acaattagca cggcgttgca ttgtgaaaag acgagaagga
    10081 taacgtatga gcgaattttt taataagacc aacgaaccag attactggga gacggctgcc
    10141 aaccagcatg ttaatcgagc aaaaactgcc cggcgggaag tgatggttca tgctggagag
    10201 ctgcataggg cggcacaaga gttggcgggc cgacttactg atagaggagt tcctcctaat
    10261 atcactgtcg tggcgacaga agtcgaaaaa gcagggcgag ccaggactgc ctggcgcaag
    10321 cagtttcgct ggcaggaaga tccgcatcac tatcccgttg gggcccaggt actgggtcga
    10381 gggtggctga tttcccagac atccgttcga tctggtaaag ccaactctcg gagcataaca
    10441 cttctcgaag acggaagttt agtaatcagt ggtccgacta cgggcgaagg ctcggcgaga
    10501 caggccgagg cgtatcgcga gccggcacct ggcttggaaa gtgtcccagg aaagacaata
    10561 ctcggcagaa ataaaaatat acatggtact gaaaagcgta agtggcaaga ggcgccacgg
    10621 ggagccgtcc gagctgctat tgacctcaca acctttccga cacatccctt atttgcaacc
    10681 gaaaagagct atgcaaacac gacgctcggt atgcccgatt taggagcgaa tccggatagt
    10741 gctgtggacc cccacacgcc tgaggcatat gtagcagcta acctcgaacg attggaacat
    10801 gacttaagaa gtacgggccg tgaattattg gggtacgaca ggccttaatt gtcgattgta
    10861 gcgataccaa ataaggggca tcaaagctcg gctgccctag ttttggcgac ttataggagg
    10921 aggaccttgg gagggtctgg tatcggaaga ggggtcgagc gtttggtaaa cccctgtttc
    10981 caccgccagc tggcgcaccc tctcggcaac atgcgcggca cccggagtgt ttgtctggac
    11041 ggttgggccc aggccgccag ctgctcgcgg ttggcctaag ttcactgcgc cgcgggcgac
    11101 cgaagccggt atttgtggcc ctgcctgctg tgtgcctgtt gctgggccct gcgctacctg
    11161 ttgttgcacg gcagctgctg cggcggcctg ttgctgcatc gtggctgcta gttctgcccc
    11221 ctcgccgccg ataccactac cgccacccca acggctaaca aaactctcgg caatactggc
    11281 cgcttgaccc tgcatacgtt tggcttggtc aatttcatct tgccgagctt gacccgtcag
    11341 accagtcgga ctgttagcga ttacagcttg agcatctctg tttgcctgga tgacttggcc
    11401 aatcgtaccc gcatgcatct gagccatgtt atagctacct cgcttttcgt aaatctcaga
    11461 cataaagctt tctctttggt cggcatccat ctggccagtt tgccagttgg tgtacttaaa
    11521 ttggagatgc ttgtcttggt tctggaaggc tgcgccgatc caggcgccgg ctgcctgctg
    11581 atcggttagc ccgttagcct gggcaacgtt gtgataatca gaagcaatac gctgcacatc
    11641 ttgctctgta tcggccttgc gcatttcata agccagcgac gcttgcatgg cataggcatt
    11701 tcctccccag cggcggtggc cggcgtcaac atcggcttca ttaatccaag cgccgcccag
    11761 cgacttgaat tgtttccggc cgtcactagt tgtgcgtgac tgtgcatatc gcttgtcaac
    11821 cgaaaggccg ccgatttcat cgtccttgcc ggtggcgatt atgtcattga actcttttcc
    11881 tatctcggca ttagctgccg aagagcgggc ttcaatgttg tacccgccaa ctgccctggc
    11941 gagtccgccc aggcctcggc gccgaaaagc accgcctcgg ctggctgaat cctgcagggt
    12001 tgaataccaa ttctgacgcc cctgtataga agcgcgagtg agatggctct gggcgcgttg
    12061 acgacgaccc tcggttgctt tgtgggcgcg gtcgcggaca ctcgtcacgg cgtcatgggc
    12121 acggcctaag aagccgccag ccaagcggaa gctgtacggt atcaacagga gcggcacaaa
    12181 aagggctatg ataccgagga gttgggccag ggttccatcg atcaacccac tcgaagttga
    12241 actcatggtt acagaaagaa cgttcccaat tgcaaaagca gcggcaataa tcgggtatac
    12301 gatcaaggtc ctcagcagta agtcccacca ctttctgaaa tactgctctg tgtttggtaa
    12361 acagtagagg gcaaaagcca ccggcgagat aagtattaga aacaagatca ggccacgccg
    12421 taagataacg gtgaccaaaa tagctataaa tgtaagaaag gccggtagta caactacaaa
    12481 ggcgaggaac tcaagcaagg caggtgcgag ggacagcttc caaataagtc ctgcggagcc
    12541 aagtagggcg cccataccca catcgcccgc cgcccctccg atatggagct taaaagcgcc
    12601 agcattttta aagggaccct cgattagggc ttcaacccca ttgccaacta cattggtaat
    12661 atcaactgcg agcgctacaa gatagataga taaattaatt aatactgctg cgatcaggag
    12721 acggggtaag at
//
LOCUS       DATNGP010000188         4283 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_524646, whole genome shotgun sequence.
ACCESSION   DATNGP010000188 DATNGP010000000
VERSION     DATNGP010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4283
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_524646"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..293
                     /locus_tag="VN778_04890"
     CDS             <1..293
                     /locus_tag="VN778_04890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50334.1"
                     /translation="EVKAAKVILVAPWLNRSHEEDATFFDFELRPEIVDQVISITVFA
                     SDNDHKDVTDSVDYLKTMLPGARFVTFHGYGHFCFEDMKTTAFPELLASVLP"
     gene            352..1464
                     /locus_tag="VN778_04895"
     CDS             352..1464
                     /locus_tag="VN778_04895"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015796175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXR50335.1"
                     /translation="MNITFFGTYDTRTTPRIQVMIDGLRSSGIDVQECNVPLKLSTAE
                     RVKILRQPWRLPVLGGKIMLCWLRLTKRSRQVSSTDAVIVGHLGQFDIHLARWLFPRK
                     LLALDYMISGSDTAKDRHLSGGLKDKLLVWLDNRALAAANLVILDTEEHKAALPDTYR
                     DKSVVVHVGAPQQWFDAAKYPRDTHRPVRIIFFGAYTPLQGAPVIGQAIGRLNTPVAI
                     TMVGTGQDEAATKRAVSKNKGGANVTWIDWIESAKLPEVVAEHDICLGVFGTGPKAFR
                     VVPNKVYQGAAVGCAIITSDTPPQRRVLQSGTVLVPYGDAEVLAAAIEDLARHPKKLL
                     KLRQAAYRLGHTSFTPHEITRPLAARIKREVSAATK"
     gene            complement(1477..2106)
                     /locus_tag="VN778_04900"
     CDS             complement(1477..2106)
                     /locus_tag="VN778_04900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50336.1"
                     /translation="MAHDIKVAASALATAIALVAYVPYLIDMFRGKNRPHLYTWISIF
                     LVTAVVAYIQLIGGAGQGAIPTTLGVGVDAIILFYCFKFGTKDVVFMDKVCLAITILG
                     VITYALFNSQPVFSLAVVTAAEIISFIPTFRKTHNDPYSESLPSYYFLIAKLSLILVA
                     LQTYNLLTVSYSALWLVVIITFLISVYGWRHGKRKPRNKELGISISPGY"
     gene            2157..3113
                     /locus_tag="VN778_04905"
     CDS             2157..3113
                     /locus_tag="VN778_04905"
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HXR50337.1"
                     /translation="METINKQHHQNKLTYLGVAWIDVQDPDDETLEDLKQKYSLHPIH
                     LQESTQAVQHNQVERGKGYLFFVIHFPVYHAAASKITFGQLGIFLGKDYVVTIRNKED
                     AVLEAFYRAVADPEIGKTHFGRGAAYLLYLILDKLLGELATFTYKVVNELDDLEDDVF
                     DSNGSDAQEIGKMRQKIVRLMQLISPKRAIMGDLTEQVGSFAGHDVAKYYSNEAKTVN
                     KLWEVINEAKETVEIYKDADFTISTEQTNQILTVLTLIFTFTIPVTVFGALYGMNVLL
                     PGGIEAGSWTFFGRYTMFAIVVALSVLSAIGMYIYFKKRRWI"
     gene            3171..3764
                     /locus_tag="VN778_04910"
     CDS             3171..3764
                     /locus_tag="VN778_04910"
                     /inference="COORDINATES: protein motif:HMM:NF017219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan amidohydrolase family protein"
                     /protein_id="HXR50338.1"
                     /translation="MNLTSDEVRTLQHRPLFNLLSQIILSAGQPMPRYYLQKPDGGMI
                     NVMEYTQQADGSMTYVKDSGNGCAVYVCRMLMLTTLDVQNTRRLIDGVHGTVTSSVRA
                     LLAAGWQDVNIQEPPRPGDIIVWEKQQQADGEHVHIGFSLGNNEAISTQFMRMNIGRH
                     SVTYDGSRRIERLLRYPWPAEEYDALVHLDLADGSAR"
     gene            3783..>4283
                     /locus_tag="VN778_04915"
     CDS             3783..>4283
                     /locus_tag="VN778_04915"
                     /inference="COORDINATES: protein motif:HMM:NF022114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gmad2 immunoglobulin-like domain-containing
                     protein"
                     /protein_id="HXR50339.1"
                     /translation="MNTDEVPTMTDLRIGKMNPFIVAILGVLLIATTGVGVYLWQQET
                     IDKRDTAITSLQAQLAEAQNEAAKPSTANHYTSTKGVSVVIYAPLKDAQVSNPTGIIG
                     EVPGGWSFEAAFPVELQDSTGAVIAHGSAQVLGDWMSPSGLIPFSVKLIYADAPKGTG
                     TIILRKD"
BASE COUNT         1103 a         1047 c         1145 g          988 t
ORIGIN      
        1 ccgaagtaaa agcggcgaag gtaatcttag tcgctccgtg gctcaacaga agccacgaag
       61 aagatgcaac attcttcgac tttgagcttc gccccgagat cgtggatcag gtaatcagca
      121 ttacggtatt tgcctcggac aatgaccaca aggacgttac ggactcagta gattatctaa
      181 aaactatgtt gcctggtgct aggttcgtta cctttcacgg ctatggtcat ttctgctttg
      241 aggacatgaa gacgactgct tttcctgaac tactagcctc ggtgctccct tagtcgctat
      301 gcccggtttc gcctagggta tactactagc agaatcttag aggccgacat agtgaacata
      361 actttttttg gaacctacga caccaggaca acgcctcgca tccaggttat gattgatggg
      421 ctgcggagca gcggcataga tgtgcaggag tgtaacgtgc cgctcaaact cagtacggca
      481 gaacgcgtaa aaatattacg gcagccctgg aggttgccgg tactgggcgg caaaatcatg
      541 ctttgctggc tgcgtctcac gaagcgctcg cgtcaggttt cctctacaga tgcggttatc
      601 gtggggcatt tggggcagtt cgatatccac ttagcccgct ggttattccc gcggaagttg
      661 ctggccttgg actatatgat atccggcagc gataccgcca aagatcgcca cttaagcggt
      721 ggactcaagg acaagctgct cgtgtggctc gataataggg cgctcgcagc agcgaacctc
      781 gtgatcctcg atacggaaga gcataaagcc gcgttgccag atacatatcg cgacaaaagc
      841 gtcgtggtcc atgtgggagc tccccaacaa tggtttgacg cggcgaaata cccgcgcgat
      901 acgcaccggc ccgtgcgtat tattttcttt ggcgcctata cacctttgca gggcgcacct
      961 gttattggtc aagcaatcgg caggctaaac acaccggtcg caattactat ggtgggcacc
     1021 ggccaggatg aagcggcaac aaagcgggca gtctcaaaaa ataagggtgg cgcgaacgtc
     1081 acctggatag actggatcga gtcggcaaaa ttgcccgagg tggttgctga acatgatatt
     1141 tgtttagggg tttttggtac ggggccgaag gcgtttcggg ttgttccaaa taaggtttat
     1201 caaggtgcag ccgtagggtg tgcgatcatc acctcggata caccgccgca gcggcgagtg
     1261 ctgcaaagcg ggacggtact ggtgccttat ggtgatgccg aggtgctagc tgctgctata
     1321 gaggatttgg cacggcaccc gaagaagctg ctaaagctta ggcaggccgc ctataggctt
     1381 ggacacacga gctttacgcc acacgaaata accaggccgc tcgcggcgcg gataaagcgc
     1441 gaagtttctg cggctacaaa atagtttctt gtaagctcag tatcctggac tgatgctgat
     1501 tcctagctct ttgttcctgg gttttcgttt gccatggcgc cacccgtaaa ccgagatcaa
     1561 gaacgtgatg attacgacca gccagagtgc ggaatatgat accgttaata agttgtacgt
     1621 ctgaagcgca acgagaatta agctgagttt agcgatcagg aagtagtagc tgggcaggga
     1681 ttccgaatac gggtcattat gtgttttccg gaaggtcggg ataaatgaaa ttatctctgc
     1741 cgcggtaacg acagccagcg agaaaaccgg ctgactatta aatagagcat atgtaattac
     1801 accaaggata gtaatggcta aacaaacctt gtccatgaat acgacgtctt tggtgccgaa
     1861 cttaaagcag tagaagagaa ttatagcgtc aacaccaacg cccagagtag tcggaatggc
     1921 accctggcct gcgcccccta taagttgaat gtaagcaaca acagctgtaa caagaaagat
     1981 gctaatccag gtgtaaagat gcggtcgatt ctttcctcgg aacatatcga tcagataggg
     2041 aacgtaggcg acgagcgcaa tagcggtggc tagtgccgat gcagcaactt ttatgtcgtg
     2101 agccataact ttataaagaa tagcagttta agccgggttt gctatgatga acctatatgg
     2161 aaaccataaa caaacagcac caccaaaaca aactgacgta tcttggcgta gcgtggattg
     2221 acgttcagga tcctgacgat gaaactctag aggacttaaa gcaaaaatat agcttgcatc
     2281 caatccattt acaagaaagc acccaggccg tccagcacaa ccaagtcgaa cgtggcaagg
     2341 gttatctatt ctttgtcatt cactttccgg tgtaccacgc cgcggcgagc aagatcacgt
     2401 tcggacagct aggcattttc ttgggcaagg actatgttgt tacgatccgc aacaaagaag
     2461 atgccgtgct ggaggctttc taccgcgccg tagccgaccc ggaaattggc aagactcact
     2521 tcggtcgcgg cgcggcctat ttgctctacc tgatccttga taaattactc ggagaactgg
     2581 cgacgttcac gtacaaggtg gttaatgaac tggacgacct ggaagacgat gtctttgaca
     2641 gcaacggttc cgacgcgcag gaaatcggca agatgcgcca gaaaattgta cgcctgatgc
     2701 agctcatcag tcccaagcgg gcaattatgg gtgacctgac cgagcaggtt ggatcattcg
     2761 ccggccacga cgtggcgaaa tattattcca atgaggctaa aaccgtcaac aaactatggg
     2821 aagtcatcaa cgaggccaag gaaacggtcg aaatctataa agacgccgat tttactatca
     2881 gcaccgagca aaccaaccaa atattgaccg ttctgacact gatatttacg ttcacgattc
     2941 cggtcaccgt attcggtgca ctgtacggca tgaatgtgct gctgccgggc ggcatcgaag
     3001 cggggagctg gacattcttc ggtcgctaca cgatgtttgc gatcgtcgta gcgctatccg
     3061 tactgtcagc tatcggcatg tatatttact tcaaaaaaag gcggtggatc tagcccccag
     3121 cagaccttgt ccagactgtc agctctaggc cgcatgttat actgctgaat atgaatctaa
     3181 cctctgatga ggtgcgcaca ctgcagcacc ggccgttatt taatctacta agccaaataa
     3241 ttttaagtgc cggtcagcca atgccgcgct actaccttca gaagccggat ggcggcatga
     3301 ttaacgtcat ggaatatacc cagcaagccg acggcagcat gacttatgtg aaggattcgg
     3361 gtaatggctg tgcggtttat gtctgccgaa tgctgatgct cacgacgcta gacgttcaga
     3421 atacccgccg gcttatcgac ggtgtgcatg gtacggtcac ttcgagtgtt cgtgcgctgc
     3481 tggccgccgg atggcaagat gtaaacattc aggagccgcc caggcccggc gatattatcg
     3541 tttgggaaaa gcagcagcag gcggatggag aacacgtcca cattggcttt agccttggta
     3601 acaacgaagc gatcagtacg cagtttatga gaatgaatat tgggcggcac agtgtaacct
     3661 acgatggcag ccgccgaatt gaacggctgc tacgttaccc gtggccagcc gaagaatatg
     3721 atgcactggt gcacctcgat cttgctgatg gcagcgcgcg ttagctggta tactggaagt
     3781 gtatgaatac tgacgaagtg ccaactatga ctgacctgcg tatcggtaag atgaatccgt
     3841 tcatagttgc tattctcggt gtattattaa tcgctactac aggcgtggga gtttatttgt
     3901 ggcagcaaga aaccattgat aagcgcgata cggcgatcac ctcgctgcag gcccaacttg
     3961 ccgaagcaca gaatgaagct gccaagccgt ccacggccaa ccactacacc tctaccaagg
     4021 gggtctccgt cgttatttat gcaccgctaa aggacgcaca agtcagcaat cctacgggaa
     4081 taatcggcga ggttcctggc ggctggtcgt ttgaagcggc atttcccgtt gaactgcaag
     4141 atagtaccgg cgcggtgatt gcccacggtt cagcacaggt acttggcgat tggatgtccc
     4201 catccgggct tatccctttt tcggtcaaac tgatctacgc tgacgcgcct aaaggtaccg
     4261 gtaccataat cttgcgcaaa gac
//
LOCUS       DATNGP010000189         2650 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_3304193, whole genome shotgun sequence.
ACCESSION   DATNGP010000189 DATNGP010000000
VERSION     DATNGP010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3304193"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            75..644
                     /locus_tag="VN778_04920"
     CDS             75..644
                     /locus_tag="VN778_04920"
                     /inference="COORDINATES: protein motif:HMM:NF014614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HXR50340.1"
                     /translation="MAFEDYKSKPSVVVVYTGEGKGKTSAGLGLLGRALGNRWRVAFI
                     QFIKYWGVSEHVFIRDIQPLYKDQLYFYKGGKGFYQAGELSESYVTEEQHKQAADQTY
                     AEALSAASSGDYDLVICDEVNNAVHDGLITEQQLKDLIAQRSPQTSLCLTGRNFPQDL
                     LPLVDIATEMKKIKHHFDDKFLANKGIDF"
     gene            complement(641..1426)
                     /locus_tag="VN778_04925"
     CDS             complement(641..1426)
                     /locus_tag="VN778_04925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50341.1"
                     /translation="MVEHEITESPRAHALVEQIMDTEMEGFDFRKTDIHATAIGLIPM
                     LVAKARLATAHYGDSYRGFLVGAAAVAYDTSPDNPRIGIYTSGNFKATHDEEDYDPTH
                     DAVDIPKVCAEMDILLRADHDEMQRLGIFVVAATTNHQRILEVTKVRSATLHPCWDCE
                     ETMKGSSLVDNDTIILTVGAAKDVYQAQSYAEFRKRYKQVRHGNDFEDAETHEYRAAD
                     WPFRQLLYKQLVKKHGRGGNVYSPDARRELKRRKLALTALRAT"
     gene            1500..2012
                     /locus_tag="VN778_04930"
     CDS             1500..2012
                     /locus_tag="VN778_04930"
                     /inference="COORDINATES: protein motif:HMM:NF020022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXR50342.1"
                     /translation="MHIPRLYLFVGYPGAGKTSVARIIHEKTGAVHLWADQERLKTFG
                     KPTHSPDESLQLYTYLNELTDKLLSEGKSVVFDTNFNFRKDRDHLRQIADKNDAETVI
                     VWIVTPKALAKQRSVHDQNLRNGYEFIMTENDFERIASHLETPDEDEKIIKIDGTKID
                     KQQVIELLHL"
     gene            2014..2598
                     /locus_tag="VN778_04935"
     CDS             2014..2598
                     /locus_tag="VN778_04935"
                     /inference="COORDINATES: protein motif:HMM:NF012774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HXR50343.1"
                     /translation="MSYHYRRKHGWAALVIILLSWLVFYAQAQGWLHGAGTSIQSSQP
                     GLYRVARFIDGDTIVVDMNGTQEKVRLIGVDTPETHKPNTPVQCYGPAAAAYTKNTIG
                     ATHVRLVSDSLSTNRDRYNRLLRYVYLPDGTNVDERLIQNGYGFYYPYFPFTKAHQFS
                     VDEQAAMAAHKGLWGNCHPTPTDQGGYKMDEIQN"
BASE COUNT          717 a          653 c          617 g          663 t
ORIGIN      
        1 tcgtaccgcc agcctggaaa cctttctctc atctgatccc ctttgtggtg actattatag
       61 taacattata ggcaatggct tttgaggatt ataaaagcaa accgtctgtc gtagttgttt
      121 acacgggcga gggtaaaggc aagacttcgg ctggcctcgg ccttttagga cgggcgctcg
      181 gtaaccgttg gcgggtagct tttatccaat tcattaaata ttggggtgtt agcgagcacg
      241 tctttattcg tgacatccaa ccactctata aagaccagtt gtacttttat aaaggcggta
      301 agggcttcta ccaagccggt gagctcagtg aaagctacgt taccgaagaa cagcacaagc
      361 aagctgccga ccaaacctac gccgaagccc tgagcgccgc cagcagcggc gactatgatt
      421 tggttatttg tgacgaggtt aacaatgccg tgcatgacgg gctaatcacc gagcaacagc
      481 tgaaggacct gattgctcag cgctctccgc agaccagcct ttgcctgacc ggccgcaatt
      541 tcccccaaga tttgctgcca ctggtagaca ttgccaccga aatgaaaaaa atcaaacacc
      601 atttcgatga caaatttctt gccaacaaag gtattgattt ttaagttgcc ctcagggcag
      661 tcaaagctag ttttctgcgc ttaagttcac gccgcgcatc aggagagtag acattgcccc
      721 cacgtccgtg cttttttact aactgtttat agagaagttg tctaaaaggc cagtctgcag
      781 ctcgatactc atgtgtctcc gcatcttcga agtcgttgcc atgtctcacc tgcttatatc
      841 gcttgcgaaa ctctgcataa ctttgagcct ggtatacatc tttcgccgct ccgacagtta
      901 gaataatggt atcgttatcc actagtgagc tacctttcat cgtttcttca caatcccaac
      961 aaggatgcaa cgttgcagaa cgcactttcg ttacttccaa aatacgctgg tgattcgtgg
     1021 ttgcggctac gacaaatatg ccgagacgtt gcatctcatc atggtcggca cgtaataaga
     1081 tgtccatctc cgcacatacc ttcggtatgt caacagcgtc atgggttgga tcataatctt
     1141 cctcatcatg cgtagcctta aagttaccag aggtataaat cccgatgcgc gggttatcgg
     1201 gagaagtgtc ataggcaacc gctgcagctc ctaccaagaa tccccggtat gagtcaccat
     1261 aatgcgcagt ggccaggcgg gctttagcga ccagcatcgg gatcagacca atagcagtag
     1321 catgtatatc ggtcttacga aaatcaaagc cctccatctc agtatccatg atctgctcaa
     1381 caagagcgtg agcccgggga gactcggtga tttcatgctc aaccatttat acatatttta
     1441 acaccttaat tataaattaa caagaattta taccgtgtac atttgctaca ataagcagca
     1501 tgcatatacc tcgactttat ctgtttgtgg gctatcccgg agcagggaaa acctccgtgg
     1561 cgcgcattat tcatgagaaa accggagcgg tacatttatg ggcagatcaa gagcgactga
     1621 aaacgtttgg caagccaacg cattccccag atgaaagctt acagctctat acctatttaa
     1681 acgaactgac tgacaagctg cttagtgaag gcaaaagcgt ggtttttgat actaatttta
     1741 atttccgcaa ggaccgggat catttgcgcc aaattgctga caaaaatgac gcagagacag
     1801 tgattgtttg gatagtaacg cccaaggcgc tggccaagca gcgctcggta cacgaccaaa
     1861 acttgcgcaa cggctatgag tttataatga ccgaaaacga ctttgaacgt attgctagcc
     1921 acctggaaac gccagacgaa gatgaaaaaa ttattaaaat tgacggtacg aaaattgaca
     1981 agcagcaggt gatcgagtta ctacatctgt agtatgagct atcattatcg ccggaaacat
     2041 ggttgggcag cgctggttat tattctcctc agttggctag ttttttatgc gcaggcgcaa
     2101 ggttggctac acggcgcagg cacatcaatc caaagcagcc agcccgggct ttaccgggtt
     2161 gctcgcttca ttgacggcga tacgatcgtt gttgacatga acggtacgca agaaaaagtt
     2221 agattaatag gcgtggatac gccagaaact cacaaaccca acacgccagt gcaatgttac
     2281 ggcccggctg cggctgctta caccaaaaat actatcggag ccactcatgt acgcttagtg
     2341 tctgactcgc tgagcaccaa tcgcgaccgt tataaccgct tgttgcgtta tgtctatctg
     2401 ccggatggta cgaacgtcga cgaaagactt attcaaaacg gctacggctt ttactatcca
     2461 tatttcccat ttaccaaagc tcaccaattt tcagtcgacg aacaagccgc catggctgcc
     2521 cataaagggc tctggggtaa ctgtcatccc acgcccactg accagggtgg ttacaagatg
     2581 gacgagatcc aaaactagtc aacgaaggta gccgggttga cgcgtggcga ctctatggga
     2641 gtgaggagtt
//
LOCUS       DATNGP010000190         2235 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_2370746, whole genome shotgun sequence.
ACCESSION   DATNGP010000190 DATNGP010000000
VERSION     DATNGP010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2235
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2370746"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..567
                     /locus_tag="VN778_04940"
     CDS             <1..567
                     /locus_tag="VN778_04940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50344.1"
                     /translation="NYFVVISRNNELANMTVDSQNLLRTTVENIRFGNGVSQSNLISD
                     PNAPSGGWSTSNSSFVIVLEVPAETTSRSYIIDPDTGSPYMNELVYYKNGSTLMERQL
                     ANPSATGNNMTTTCPASLATASCPADIQLAQYVNSMTFTLYDQDAAVTTTPSDARSIY
                     ITLNMRRNDPGAPINLTTSTRVTLRNRF"
     gene            570..1922
                     /locus_tag="VN778_04945"
     CDS             570..1922
                     /locus_tag="VN778_04945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50345.1"
                     /translation="MKQRLADNQKGSIILVFIITLPFLILLAMYYMSLSLTSFQVSRL
                     DQLHTEAQLAADAGADYAVEQLAQNTSWAGTGSEITLHSDSSLRTTYTATVTGSSSSK
                     TIAVTGNTYWPASATTPTRSVSVDIDLYPVTTGNYSVVAGAGGLFMSNSSKITGGSVF
                     INGEISMSNSAQIGLSTNPVSVQVADDICPSPADSTYPKVCTSGNPITINNTAHIYGA
                     VTATNQTNGSGMSNTGLTSGGSVAPQALPTYTRPDSSHYPNSLTGNQASCSGSQAVTW
                     PAGTKITGNVTLSNSCTVTVQGDVWITGTLNLSNTTKMIVSNTVGSTQPVIMIDGQGG
                     ATFSNSTQIVSNSSSTGIEFITFYSMAACSPSCASVTGTDLANSRSISTITLSNTAAA
                     PNSVFYAYWTQVQLSNSGQLGAVIGQTIQMSNTAAITFGSAQAGVGNTTWIVKGYHRQ
                     "
     gene            1982..>2235
                     /locus_tag="VN778_04950"
     CDS             1982..>2235
                     /locus_tag="VN778_04950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50346.1"
                     /translation="MKTTNTPNFFHDKPVFGLDIGHGSLKVMQVEHPAPGVPGKKNSP
                     RVVGYGFTNFDKAAQEDGVIVQPEIIAKATMELFERHLIG"
BASE COUNT          574 a          628 c          523 g          510 t
ORIGIN      
        1 aattactttg tagttatcag ccggaataac gagctcgcta atatgacagt ggattctcaa
       61 aacttgctgc ggaccactgt cgaaaatatt cgctttggca acggcgtgag tcagagcaac
      121 ttgatcagtg atccgaatgc accttccggc ggctggagta ccagcaacag ctcttttgtg
      181 atcgttttgg aagtccccgc agaaactacc tcgcgcagct atattatcga tcctgacacc
      241 ggcagcccgt acatgaatga gcttgtttac tataaaaacg gctcaacact gatggagcgc
      301 cagctagcta acccaagtgc tacaggaaat aatatgacta ctacttgccc agccagcctg
      361 gcaaccgctt catgcccggc cgatattcag ctggcccaat acgttaatag catgactttc
      421 accctgtacg accaagacgc tgccgtcacg actacccctt cggatgctcg ctccatttat
      481 attaccctca acatgcggcg taacgatccg ggagcgccaa ttaacctcac caccagcact
      541 cgagtaacat taaggaacag gttctaaaga tgaaacagcg tttagccgac aatcaaaagg
      601 gttcaattat tctggtattt attattaccc tgcccttcct gatcttgctg gctatgtatt
      661 atatgagttt gtcactgacg agctttcagg tgagtcgatt ggaccaactg cataccgagg
      721 cccagctcgc ggctgacgcc ggggctgatt atgccgtaga gcagctggcc caaaacacta
      781 gttgggcggg gactggcagc gaaattaccc tgcatagcga tagttctctt cgtaccacct
      841 atactgctac tgttacaggc tcgagcagct ccaaaactat tgccgtgacc ggcaacacct
      901 actggcctgc ctccgcaacc acaccaacgc gttcggtgtc agtcgacatt gacctctatc
      961 ccgtaacaac cggcaattac agcgtcgtgg cgggcgccgg gggtttattt atgagtaata
     1021 gctccaagat caccgggggc agtgtcttta tcaacggcga gattagcatg agtaattcgg
     1081 cgcagattgg cctcagcacc aaccccgtaa gcgtgcaggt ggctgatgac atttgtccga
     1141 gcccagctga ttccacctac cccaaagttt gtaccagtgg caacccgatc accatcaaca
     1201 ataccgcgca tatttacggt gccgttacgg caaccaatca aacaaacggc agcggcatga
     1261 gcaataccgg cttgacttcg ggtggcagcg tcgccccgca ggctttaccg acttatactc
     1321 gccccgattc cagccattat ccgaatagcc ttaccggtaa ccaggcttct tgttctggca
     1381 gccaagctgt tacgtggccg gccggcacca aaattaccgg caatgtcacc ctctccaatt
     1441 cctgcaccgt caccgtccag ggtgacgtat ggatcaccgg caccttaaac ttgagcaata
     1501 ctacaaaaat gattgtcagc aataccgtcg gctcaaccca gcccgtcatc atgattgacg
     1561 gccaaggcgg agcaactttt tccaacagca ctcaaatcgt atcaaatagt agcagcacag
     1621 gcattgagtt cattaccttt tatagcatgg ccgcttgctc gcccagctgt gccagcgtaa
     1681 ccgggacgga cctcgcgaac tcccggtcca ttagcacaat caccctcagc aacacggccg
     1741 cggcgccgaa cagcgtattt tatgcttact ggacgcaagt acaattgtcc aactcaggtc
     1801 agctaggggc agttatcggg caaaccatcc agatgtccaa taccgcggcc attacttttg
     1861 gttcggcaca agcaggcgtt ggtaatacca catggatcgt taaagggtat catcggcagt
     1921 aaagataagc tttatctggt atactaatga agactcagga agcctctggt gggcaggtgc
     1981 aatgaaaaca actaatactc caaacttttt ccacgataag ccggtcttcg gtttagatat
     2041 tggccatggc tcgctcaagg taatgcaagt tgagcatccg gcacctggcg tacccgggaa
     2101 aaagaatagc ccccgagtgg tcggctacgg ctttaccaac ttcgacaaag cagctcagga
     2161 agacggggtg atagttcaac ctgaaattat cgccaaagcc actatggagc tcttcgagcg
     2221 tcatctgatc ggtga
//
LOCUS       DATNGP010000191         2853 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_2389145, whole genome shotgun sequence.
ACCESSION   DATNGP010000191 DATNGP010000000
VERSION     DATNGP010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2853
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2389145"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..758
                     /locus_tag="VN778_04955"
     CDS             <1..758
                     /locus_tag="VN778_04955"
                     /inference="COORDINATES: protein motif:HMM:NF013260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXR50347.1"
                     /translation="AHDYDNLVTIRPLRAPHHSASLVALVGGGHSLRPGEISLAHRGV
                     LFLDEMPEFSRAALESLRQPLEDRLISVSRAKDTVNYPADFILMATANPCPCGYYGSA
                     KPCTCLPHQITQYQNRISGPILDRIDLHSTVQDVSHRDLLRSAEDSKEDRDVLERINR
                     ARKTEANRYDTASRLNARMTNRDIKTFAKLEPSAKTMLDTAAERLMISARSYMKTVKV
                     ARTIADLDDSSAIEASHIAEALQYRRNEQPLVG"
     gene            883..1401
                     /gene="infC"
                     /locus_tag="VN778_04960"
     CDS             883..1401
                     /gene="infC"
                     /locus_tag="VN778_04960"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HXR50348.1"
                     /translation="MNGAIQAPELRVVDEDGKQLGILSRREALDLAQERELDLVEISP
                     DAHPPVVKIVDWGKYSYQKTKQMQRNKKNAKALEMKQMRFGLKIGDHDLQVKLGKVTG
                     FLEAGHKVKLTVVYRGRELAHKDLGFKLADKIVAEFGDQVVVDQQPIFAGKQLNLVIR
                     GTHAHAAPTNHD"
     gene            1462..1659
                     /gene="rpmI"
                     /locus_tag="VN778_04965"
     CDS             1462..1659
                     /gene="rpmI"
                     /locus_tag="VN778_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012867226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HXR50349.1"
                     /translation="MPKLKTHSGTKDRVRITRNGKIRVAHPNMSHFLQKKSGGRKRSL
                     SGLRTVLHAATRHTIKKQLGA"
     gene            1664..2008
                     /gene="rplT"
                     /locus_tag="VN778_04970"
     CDS             1664..2008
                     /gene="rplT"
                     /locus_tag="VN778_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HXR50350.1"
                     /translation="MRVKRGLASHKRHLTIRHATKGMSHPNRASVKRGRQAVTKALQF
                     AYRDRRNRKRTFRELWNARINAAARLHNTTYSRLIASLKAANISLDRKVLSELATNEP
                     KAFEAVVKAAQK"
     gene            complement(2062..2501)
                     /gene="rnpB"
                     /locus_tag="VN778_04975"
     ncRNA           complement(2062..2501)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VN778_04975"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(2538..>2853)
                     /locus_tag="VN778_04980"
     CDS             complement(2538..>2853)
                     /locus_tag="VN778_04980"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF024848.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HXR50351.1"
                     /translation="GIYLGVTTNNQAEYTALKQALEEAKSLGGQEVHVYMDSLLVVNQ
                     MRGIFKVKNRDLWPLHDAVKQLVTQFEKVDFTQIPRELNKLADGAVNAALDQHQAEQA
                     TK"
BASE COUNT          719 a          816 c          720 g          598 t
ORIGIN      
        1 cagctcacga ttatgacaat ctggttacca ttcggccctt gcgcgcaccc caccatagcg
       61 ccagcctcgt agcactcgtc gggggtggac atagtttgcg gcccggtgag ataagcctag
      121 cacaccgtgg cgtgctgttt ctcgatgaga tgccagagtt tagccgcgcc gccctcgagt
      181 cactgcgcca accgctggaa gatcggctga taagcgtctc tcgagccaag gataccgtca
      241 actatccagc cgatttcatc ctcatggcta ccgccaaccc gtgcccgtgc ggatactacg
      301 gctctgccaa gccctgtact tgcctgccgc accagatcac acagtaccaa aatcgtatct
      361 caggcccgat cttagatcgt atcgacctac acagtactgt ccaggatgtt agccaccgcg
      421 acttgctccg cagcgcggag gattccaaag aggaccgtga cgtgctcgaa cgcattaacc
      481 gtgctcggaa aacagaggcg aaccggtacg atacggctag taggctgaat gcccgcatga
      541 cgaaccgcga tatcaaaacg ttcgccaagc tcgagccgtc ggccaagacc atgctggata
      601 cggcggccga gcgtctgatg atctcggcgc ggagctacat gaaaactgtc aaagttgcgc
      661 ggacaatagc cgacttggat gatagcagcg ccatcgaagc cagccatatt gctgaagcac
      721 tacaataccg tcgcaatgag cagccgttgg tgggctgaaa atcgcggtgc aacaacaatg
      781 ctaggcaaaa gcctagttga cccctgttgg gtgatctggt acactgaaac cattagctac
      841 taagagcgcc gaaggagaaa acgtatcagt aaacctaccc gcctgaatgg cgccattcag
      901 gcacccgagc tcagagtcgt tgacgaagac ggaaaacagc tcggcatttt atcgcgccgt
      961 gaagcgcttg acctagcaca agaacgggag ctcgatctgg tggaaatctc acccgacgcc
     1021 cacccgccag tagtcaagat cgtcgactgg ggcaaataca gctaccagaa gacaaagcaa
     1081 atgcagcgca ataagaaaaa tgccaaagct cttgagatga agcagatgcg ctttggactc
     1141 aagattggcg accacgatct ccaggtgaag ctgggcaaag taacggggtt cctcgaggct
     1201 ggacacaagg ttaaactcac cgtcgtgtac cggggccgcg aattagccca caaagacctc
     1261 ggtttcaagc tcgcagacaa gatcgtcgcc gaattcggag accaggtggt cgtcgaccaa
     1321 caacctatct ttgccggcaa acagctcaac ttggtaatcc gtggcacaca cgcccatgcg
     1381 gcaccaacca accacgactg atacgttttt tagcagccct cgaaaagtta gcaaacatta
     1441 gcaacctaag gagagatcca catgcccaaa ttaaagaccc acagcggcac caaagaccgc
     1501 gtccggatta ccagaaacgg taaaatccgt gtcgcgcacc caaatatgag ccacttttta
     1561 cagaagaaaa gtggtggccg caagcgttca ctgagcggcc tgcgcaccgt actgcacgca
     1621 gcaacccgcc acaccattaa gaagcaatta ggagcctaaa gaaatgcgag tcaaacgcgg
     1681 ccttgccagc cataaacggc atctcaccat ccgtcatgcc accaagggca tgagccatcc
     1741 aaaccgtgcc agtgttaaac ggggccgaca agccgtcacg aaagcgctcc aattcgcgta
     1801 ccgtgatcga cgcaaccgca agcgcacctt ccgcgagctt tggaatgccc gcattaatgc
     1861 tgcagctcgc ctgcacaata cgacatatag ccggcttatc gcgagcctca aggccgccaa
     1921 catcagcctc gaccgcaagg tcctaagcga gctggcgacc aacgagccaa aagctttcga
     1981 agctgtggtc aaagctgcgc aaaagtaata gttcaatcgt tccaagctaa aaagccactc
     2041 ggtcgagtgg ttttttagtt ataggccaga ttatctgtaa gccgggtttt gtcttccggc
     2101 accagtttct gccttcgatc agtcgatgat cagattgatc gaagataaaa actggggccg
     2161 gatcactagt catctatcta gtcctatcgt tgccaatagg atcgagcggg ttcatagcgg
     2221 tcatcctgat gggacaccat ccgcttatgc agcggagtgg acgacctcct tgcagcaggt
     2281 agggtttacc ttcttgcggc gtcaccgacg caagctgtga gcttttacct cactcgtttc
     2341 atcgtagcgg tcggatatat acacccgact aacgctgtgt cgtttctgtg gcactttccc
     2401 tagggtttcc cccggttgct gttagcaact accatctccc tgattgctgc ccggactttc
     2461 ctctcgccat agtattggcg ggcgactagc cgataatctg gctatgcaaa gattataaca
     2521 cttttgctta cacttgccta ctttgtcgct tgttcggctt ggtgttggtc gagagcggcg
     2581 ttgactgccc cgtcagcgag cttgttaagc tcgcgcggaa tttgggtaaa atcaaccttt
     2641 tcaaattgag ttacgagctg ctttaccgca tcgtggagcg gccagagatc acggttttta
     2701 actttgaaaa tcccgcgcat ctggttcacg accagcaggc tatccatata gacgtgcacc
     2761 tcttggccgc cgagactttt cgcctcttca agagcctgct tcagcgcggt gtattcagct
     2821 tggttattgg tggtgacacc gagataaata ccg
//
LOCUS       DATNGP010000192         2600 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_4269113, whole genome shotgun sequence.
ACCESSION   DATNGP010000192 DATNGP010000000
VERSION     DATNGP010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2600
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4269113"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..403
                     /locus_tag="VN778_04985"
     CDS             <1..403
                     /locus_tag="VN778_04985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50352.1"
                     /translation="KTWSGYVDDTGSGSQPLDGYFNPGVVPAISGQNSVFALRVQVLS
                     QSYSQTLQSFQSAQSPSGGPALSIVPYALPKVPKVIGVEATGTLPNTNNVVGTAVVLP
                     LRSQTLEIWTMGTSYLNDFNTYILPNFIFSP"
     gene            477..1190
                     /locus_tag="VN778_04990"
     CDS             477..1190
                     /locus_tag="VN778_04990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50353.1"
                     /translation="MPREHDSQMLSEQLLLSVAEELKLPLLQIARQAEYGCLHGEADL
                     KSIQATADAALGLLDNFVLGMRLNLEHRELEVEAVSVSSVLYDAGQQLDALAKNYGVG
                     LELNIAGKFSPVIAHREGLQAALVSLGAALIEALPALEGSPQLKLQLATHRSRYGIVA
                     GMYADTQRLTNEVLNQGRKLQHLSRQPLMNVTHTSGAGIFVADSILRAMQLRLRASRH
                     HRLYGLGTVLQSSPQIQLV"
     gene            1194..1580
                     /locus_tag="VN778_04995"
     CDS             1194..1580
                     /locus_tag="VN778_04995"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXR50354.1"
                     /translation="MAHILLIEPDRVLAEILARTLFMAGHQVTPCASAQSAIMAADQA
                     RPDVVILELQLIAHSGIEFLYEFRSYLDWQHIPVLVHTGVPLGEFAASRQLMKDELGI
                     KAYLYKPSTSLKHLLRHVNQFAPSPA"
     gene            1577..2266
                     /gene="recO"
                     /locus_tag="VN778_05000"
     CDS             1577..2266
                     /gene="recO"
                     /locus_tag="VN778_05000"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HXR50355.1"
                     /translation="MNQLITKGIILSRIDYGEADRIITLLTPDHGKLRLMARGVRKVK
                     SKLAGGIELFSVSDITFIQGRGEIGTLVSTRLVKHYSEIVKHIERVQLGYDLIKLLNK
                     ATEDNPETVYFDLLQQTFEALDDSSIELELIKAWFEAQLLKEAGHAPNLQLDTTGQKL
                     TIDQAYNFDYDSMAFALQDSGSFTAAHIKALRLLFQQNILALKQVDGLLEVLPDVRGL
                     VRTFAQTYLHH"
     gene            2349..>2600
                     /locus_tag="VN778_05005"
     CDS             2349..>2600
                     /locus_tag="VN778_05005"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016482276.1"
                     /note="Catalyzes a two-step reaction, first charging a
                     glycine molecule by linking its carboxyl group to the
                     alpha-phosphate of ATP, followed by transfer of the
                     aminoacyl-adenylate to its tRNA; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HXR50356.1"
                     /translation="MSDVKLEDIVSLCKRRGFIYQGSEIYGGLAGTWDYGPLGVALKR
                     NIMQLWWKMFVEDRDDMLGVDAAILMNQKVWQASGHVDTF"
BASE COUNT          600 a          693 c          678 g          629 t
ORIGIN      
        1 taagacgtgg agtggctacg tggacgatac cggaagtggc tcgcagccct tagacggtta
       61 ctttaacccg ggggtggtac cagccattag cggtcaaaac tcagtcttcg cgctacgcgt
      121 tcaggttttg tcgcagagct actcacaaac gctgcaaagc ttccagagcg cccagtctcc
      181 ctctggtggt ccggctctga gcattgtgcc atatgccttg ccaaaggtgc cgaaagttat
      241 tggcgttgaa gctaccggta ctttgccgaa caccaataac gtcgtaggca ccgccgtcgt
      301 gttgccgctt cgctcccaga ccttggaaat ttggacgatg ggcacctcgt atctgaacga
      361 ttttaatacg tatattttgc ccaatttcat tttttctcct taacggtcaa caaactagcc
      421 gaacgcgcgc cctttgctat aatgaagcat agtaagtgag agggtagtta acattcatgc
      481 cgcgcgagca cgattcgcag atgttgtctg agcagctgtt gctaagcgtg gccgaagagc
      541 tgaagttgcc attgttacag atcgcgcggc aggcggaata cggctgtttg cacggtgagg
      601 ctgatctgaa aagcattcaa gcaacggccg acgcggctct ggggctactg gataatttcg
      661 tgcttggtat gcgcctgaat ctcgaacatc gggagctgga agttgaagcg gtgtcggtgt
      721 cctcggtgct ttatgatgcc ggtcaacaac tcgatgccct agctaagaat tacggcgtgg
      781 ggttggagct taacattgct ggtaagttta gtccggttat cgcgcaccgc gaaggcttgc
      841 aggcggctct ggtcagcctg ggagctgccc ttatcgaagc cttgccggct ctcgaaggct
      901 cgccgcagct caaactccag ttggcaacgc accgcagccg ttacggtatc gtggctggca
      961 tgtacgccga tacgcagcgg ctcaccaatg aagtgctcaa tcagggccgc aaattgcagc
     1021 acctcagccg tcagccgctt atgaatgtta cccacaccag cggtgccggt atctttgtcg
     1081 ccgattccat cctaagagcc atgcaacttc gcttacgggc ttcacgccac caccggctct
     1141 atggcctcgg taccgttttg caatcaagtc cccaaatcca gttggtataa aacatggcac
     1201 acattcttct catcgaaccc gaccgagtcc ttgcagaaat cttggctcgc acgctgttca
     1261 tggctggcca tcaggtcacg ccatgcgcta gcgcgcagtc ggcgattatg gcggctgatc
     1321 aggctcggcc ggacgtggtg attttggagc tgcagctgat cgcacattcc ggcatcgagt
     1381 ttctctacga attccgttct tatcttgatt ggcagcatat cccagtattg gtgcacacgg
     1441 gcgtaccgct tggtgaattc gcggccagcc gacagcttat gaaggacgaa ttaggcatta
     1501 aggcttatct gtataagccg tcgacatcct tgaagcatct tcttcgtcat gttaaccaat
     1561 ttgctccttc gcccgcgtga accaactcat caccaaaggt attattttaa gtcgcatcga
     1621 ttacggggag gctgatcgta tcattacgct gctgacgccg gaccacggca agctgcgcct
     1681 aatggcccgg ggtgtacgca aggtaaagtc caagctggcc ggcggcattg aattatttag
     1741 cgtcagcgat atcactttta tacagggtag gggggaaatc ggtacgcttg tctcaacgcg
     1801 cctggtgaag cattacagcg aaatcgtgaa gcatatcgag cgtgtgcagc ttgggtacga
     1861 tttgatcaag ctactgaata aagcgactga agacaaccct gaaacagtgt attttgatct
     1921 gttacaacaa acctttgaag ctttggatga ctcctctatc gagctcgagc tcattaaagc
     1981 ctggttcgaa gcacagctac tcaaagaagc tggccacgcc ccgaatctac agctggacac
     2041 gaccggccaa aaactgacta tcgaccaagc ctacaatttc gattatgact ccatggcttt
     2101 cgccctccaa gatagcggct cttttacagc agcccatatt aaagccctgc gattgctctt
     2161 tcagcaaaat atcttggcgc tcaagcaggt tgatggctta ctagaagtat taccagatgt
     2221 gcgcggccta gttcgtacct ttgcgcaaac ctatctgcac cattaggggt atttcggccc
     2281 gtacagaatc tgtatggtgg atgatgaaac tagatgagac tgccagctta cggtataatc
     2341 atctgtaaat gagcgacgtc aaactcgaag acattgtcag cctctgcaag cgccgcggct
     2401 tcatctacca gggcagcgaa atttatggcg gcctagccgg cacctgggat tacggcccac
     2461 tcggcgtcgc cctgaagcgc aatattatgc agctctggtg gaaaatgttc gtcgaagacc
     2521 gcgacgacat gctcggcgtc gacgccgcca ttctcatgaa ccaaaaagtc tggcaagcga
     2581 gtggccacgt tgacactttt
//
LOCUS       DATNGP010000193         2499 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_1459346, whole genome shotgun sequence.
ACCESSION   DATNGP010000193 DATNGP010000000
VERSION     DATNGP010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2499
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1459346"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..601)
                     /locus_tag="VN778_05010"
     CDS             complement(<1..601)
                     /locus_tag="VN778_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXR50357.1"
                     /translation="MADVILRVKDLRKNYGDLKAVDGVSFEVKKGEVFGILGPNGAGK
                     TTTLEMIETLRPIDGGTATINGIDVATRPYDIRAIIGVQPQAPGFQDKTKLTELIQMF
                     AACYGERVNPKQFLDEVNLGSKANDYVENLSGGQRQRFSIVAALVHNPKVFFLDEPTT
                     GLDPQARRNLWELVQEVRDRGISVILTTHYMDEAEVLCDR"
     gene            complement(1185..1379)
                     /locus_tag="VN778_05015"
     CDS             complement(1185..1379)
                     /locus_tag="VN778_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020023356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50358.1"
                     /translation="MAYQHTNSKGVTYYLNSKTVTLRGGKQQPIYYFSKDKRPEAADL
                     PAGMSVNENPRNGFLTVKRG"
     gene            1594..2229
                     /locus_tag="VN778_05020"
     CDS             1594..2229
                     /locus_tag="VN778_05020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50359.1"
                     /translation="MWGESFMEFMSRGSLTQQPQRMAAPEPVVRATEQHVKRGKKEDM
                     LSMGSRIGTGVLLVIIVILVALVAWFTVFSTPSSQSSYVDSSTLQAVFLNTGQVYFGN
                     VKTLNSQYFVLGNVYYLQSSNGSSASSTNSNQNVSLVKLGCELHRPYDTMVINTAQIT
                     FWENLQSDGQVAKAVAQFEQQNPNGQKCSTTSNGSSSNPQSQASTNATTKQ"
BASE COUNT          573 a          700 c          610 g          616 t
ORIGIN      
        1 cgcggtcgca gagcacttcg gcttcgtcca tgtagtgagt cgtcaggata acactgatgc
       61 cgcggtcacg gacttcttgg acaagctccc aaagattgcg acgcgcctgt gggtcaaggc
      121 cggtcgtcgg ctcatcgagg aaaaacacct tggggttgtg caccagggcc gctacgatac
      181 tgaagcgctg gcgctgaccg ccggaaagat tctcgacata gtcgttggcc ttactaccga
      241 ggttgacctc gtctagaaac tgcttggggt tgacgcgctc gccgtagcaa gctgcaaaca
      301 tttggatcag ttcagttaat ttagttttgt cctgaaagcc cggggcctga ggctggacgc
      361 cgatgattgc ccgaatatca tagggtcgcg tagcgacatc aataccgttt atcgtcgccg
      421 tgccgccgtc gattggccgg agcgtctcga tcatctcgag cgtcgtcgtc ttgccggcgc
      481 cattcggacc taaaatacca aatacttcgc cttttttgac ctcgaagctg acgccatcga
      541 cagcctttaa gtcgccatag tttttacgca ggtctttgac ccgcaggatt acatctgcca
      601 tatgcaccta ctataccaaa ttggcaggct ggttgtgcta aaaactaggg cttcgggcct
      661 ggtctcggcc taatgtcaac gcctaggaat ccgagcagcc ttaacgcata gaggtgatcg
      721 gcaagctcgt ggcgcatctt agccgcactg tccagcatta gagtaagccg ttcgggctgc
      781 agtggtataa cttctccgag gtcctcgacc gctggttcgc taacgtgacc gacaactatc
      841 gtaccttctt gcctcaacgc cgcacgcctc ctatattgct acctaaacct ggaactcatg
      901 atttggatta accgtacgga gccagcgcaa gaaattaata cgctttgcct gttccttgct
      961 gagcggctct ctactcaaag ttctgcctcg caattcgcgt agtgcatctt gttcgacggg
     1021 actaaaatct gcgagaaggg attcattttc accattatta cctagcctgt gcgcaccaaa
     1081 ctcatctgac gtttctccac tattagcgct catcactacc tcccttgtaa cgcaaaatgt
     1141 cctggttttc ggccaggaca ctcgctttag tctttgctaa atcactagcc gcgcttgacg
     1201 gtcaggaagc cgttgcgtgg gttttcattg actgacatgc cagctggcaa gtctgcagct
     1261 tccgggcgct tgtctttgct gaagtagtaa ataggctgtt gtttgccgcc gcggagtgta
     1321 accgtctttg aatttaggta gtatgttacg ccctttgaat tcgtgtgctg atatgccatg
     1381 gcacttgtct ccctctctta ttgttaggtg gtacccccaa catactctgc accgttttcg
     1441 tttgtcaaca ggtttatacg ctgtttgacc ccaagcacct ctgatgacca ctgttacata
     1501 tccgctcgca aggcatggat taatcgtaag catattgcca ccggtcgtgg atttgtacta
     1561 cactggttca gtacatacta gacatacatg taaatgtggg gagaatcgtt catggaattc
     1621 atgagtcgcg gatcattaac tcagcagcca cagcgaatgg ccgctcctga acctgtagtg
     1681 cgcgccaccg aacaacacgt gaaacgtggc aagaaggaag atatgctgtc aatgggcagc
     1741 cggattggca cgggtgtctt gcttgtcatc attgttatcc tggttgcact cgtcgcctgg
     1801 ttcaccgtat tctcgacgcc ctcttcgcaa agcagctatg tcgatagcag cacgttacaa
     1861 gccgtcttcc tgaataccgg ccaagtctac tttggcaacg tgaaaacgct caattcgcag
     1921 tattttgtcc tgggtaacgt ctactaccta cagtcgtcga acggctcatc tgccagcagc
     1981 acgaatagca accagaatgt gtcgctcgtc aagctcggtt gtgagcttca ccgcccgtat
     2041 gacacaatgg tcatcaatac tgcccagata accttctggg agaacctgca atctgacggt
     2101 caggtcgcca aggcggttgc tcaattcgag cagcaaaacc cgaacggcca gaagtgtagt
     2161 acaacttcga acggcagctc atcgaaccct cagagccaag cttcgaccaa cgcaacgact
     2221 aagcaataag tcgcagcgca acgcttgtaa atacccgctc cagtgagagc gggtatttta
     2281 taccggtcgt taacggtcaa accactaaac ctccggatca tagagagcgc cactgcatac
     2341 acgaccgtag ctttggcccg gacttgtcga ctagctctag cgacttactt tcttgagcca
     2401 ccaccggaca aacgcaaata ctccaaacgt gcccaggctc agcccggcca acaccaggct
     2461 ccagcccgct aagtcaggtg accaccaggg gctcacgaa
//
LOCUS       DATNGP010000194         2208 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_851916, whole genome shotgun sequence.
ACCESSION   DATNGP010000194 DATNGP010000000
VERSION     DATNGP010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2208
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_851916"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..795)
                     /locus_tag="VN778_05025"
     CDS             complement(<1..795)
                     /locus_tag="VN778_05025"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXR50360.1"
                     /translation="MRSLTGGHFKAGFDAIRSAKLRNFWTMLGVIIGVTSVITIVGIG
                     EGVKAQVSGQIHHLGKDLITVHPAQLRSGGANGTAVNELVDLNVASSLTNKDVDTVAG
                     TKNVGSSAPLTFVTGTLSGDSGAYKSGIVIGTTPDLPSLLNQSMAYGVFLSDNDIGTN
                     AAVLGSHAAEALFNEDVPLGRSFTFRGQQFIVRGIFNDFDSTPLSQEADFNSAVFIPY
                     DVGESLTNNTAPIYEILAKPDNSHQTTAVAASVQRALDKSHGGQSNL"
     gene            complement(792..1679)
                     /locus_tag="VN778_05030"
     CDS             complement(792..1679)
                     /locus_tag="VN778_05030"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXR50361.1"
                     /translation="MALIEVQDVSKLYGFGDATTLALDEVNLTIEKGEFVAVMGPSGC
                     GKSTLMQVLGLLDRPSHGNYRFDGRPVARLRPNQAAKIRRDGIGFIFQAYNLLPRLTA
                     IENVSLPLAYKGMSQSRRNKQASQMLERVGIQNREYFLPSQLSGGQAQCVAIARALVN
                     NPKLIIADEPTGNLDSASSRLIMELLADIHKMGNTIIFVTHNPELTRYATRVVYMHDG
                     SIIHDEKTALGQVASTARKVLYTVPTTSEEDDLAGVSALMKALPDKPASRRKRKTTTP
                     KAKSTRRTKRSTARARSSK"
     gene            1852..1941
                     /locus_tag="VN778_05035"
     tRNA            1852..1941
                     /locus_tag="VN778_05035"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1886..1888,aa:Ser,seq:gga)
     gene            2014..>2208
                     /locus_tag="VN778_05040"
     CDS             2014..>2208
                     /locus_tag="VN778_05040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50362.1"
                     /translation="MGPYCFAAYFGYAVLFIALQQLIGRHRGATNTKKREAPGFYLFS
                     LISIGFLLASTSSISHQPVRN"
BASE COUNT          489 a          578 c          585 g          556 t
ORIGIN      
        1 gagattactc tgtccgccgt gcgatttgtc cagcgcccgc tgaactgagg cggcgacggc
       61 agtcgtctgg tgcgaattat ccggctttgc taaaatctcg taaatcggag cggtgttgtt
      121 agtcaggctt tcaccgacat cgtacggaat aaaaacggcg ctgttaaaat cagcctcctg
      181 gctcagcggt gtgctgtcaa aatcgttgaa aataccgcgc acgataaatt gttgcccccg
      241 aaaagtgaaa ctgcgaccga gcggcacatc ttcattaaac agtgcctccg cggcatgcga
      301 gcccagtacg gcggcgttgg taccaatgtc gttgtcagac aggaacacgc cgtaggccat
      361 tgattgattg agaaggctgg gcaggtcggg ggtggtacca ataacgatac cgctcttgta
      421 ggcgccacta tcgccgctca gcgtaccggt gacaaaggtc aatggcgcgc tggagcccac
      481 atttttggtt cccgcgacgg tatcgacgtc tttattcgtt agactgctgg cgacatttag
      541 gtcaaccagc tcgttgacag cagtcccgtt agcgccaccg ctccgcaatt gggccggatg
      601 gacagtgatc aaatccttgc cgagatggtg gatttggccg ctgacttggg ctttcacgcc
      661 ctcgccgatg ccgacgatcg taataaccga cgtcacaccg ataataacgc caagcatggt
      721 ccagaaattg cgcagcttgg cacttctgat ggcatcgaag ccggctttga aatgaccgcc
      781 tgtcaaagat ctcatttgga gctcctggcg cgtgcggtgg acctcttggt acgacgtgtc
      841 gactttgctt ttggcgttgt agttttacgt ttgcgccgag aggcaggctt atcaggcaac
      901 gctttcataa gcgctgaaac accagccaaa tcgtcttctt ccgaagttgt gggtaccgtg
      961 taaagtacct tgcgggccgt agaagcaact tggcccaatg ccgtcttttc gtcatggatg
     1021 atggagccat cgtgcatgta gaccacccgc gtggcatagc gtgtcagctc ggggttgtgg
     1081 gtcacgaaaa taatggtgtt acccattttg tggatatcgg ccagcagctc catgatcaag
     1141 cgcgacgaag cgctgtcgag attgcccgtc ggctcgtcgg caataatcag cttggggtta
     1201 ttaacgagcg cccgggcgat ggccacgcac tgggcttggc cgccactgag ctgagaaggc
     1261 aggaaatatt cgcggttctg aataccgacc cgctccagca tctgcgaggc ttgtttgttg
     1321 cgccggctct gtgacatgcc cttgtaggct aggggcagag aaacattttc gattgctgtc
     1381 aggcgaggta gcaaattgta cgcttgaaat ataaagccaa taccatcgcg gcggattttt
     1441 gctgcttggt ttggcctcaa acgcgccaca ggccgcccgt cgaagcgata attgccatga
     1501 cttggccgat cgagcagacc caatacctgc attagcgtgc tcttgccgca gccactcgga
     1561 cccatgacgg ccacaaactc gcccttttca atagttaagt taacctcgtc gagcgccagg
     1621 gtagtggcat cgccgaagcc gtacagtttg ctgacgtctt gaacctcaat caaagccata
     1681 tcgtagtaca tagtttaaac gtaactttgc gttcactgca aggtgttttg tagtaaattt
     1741 tcctgcggtt gcatctgaac catttttaag ccctctgtac attttctgta cagaaaatgt
     1801 acgcgtgcat ttttaaagaa cggttgcctc tgtataataa tcagggttcg cggaaggatg
     1861 caggagtggt tgaactggca cgcctggaac gcgtgtatgg ccgcaaggtc atcgagggtt
     1921 cgaatccctc tccttccgcc acattgactt ttagttatat ttatagcaaa atatatatct
     1981 ccttaacaat tcatccttac atctggagac agcatgggtc cttattgctt tgccgcgtac
     2041 ttcggatatg cggtgctttt catagcacta cagcagttga tcggccgtca tcgtggtgct
     2101 accaatacca agaagcgtga agcccccggc ttctatctgt tcagcctcat cagcattggc
     2161 tttcttctcg cttcgacttc atcgattagc caccagcccg tgcgaaac
//
LOCUS       DATNGP010000195         4099 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_3032296, whole genome shotgun sequence.
ACCESSION   DATNGP010000195 DATNGP010000000
VERSION     DATNGP010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4099
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3032296"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..311)
                     /locus_tag="VN778_05045"
     CDS             complement(<1..311)
                     /locus_tag="VN778_05045"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HXR50363.1"
                     /translation="MPSLTVIRHSLALANEQGILMGAKLDSPLSKKGIEVARAKGAAL
                     KAEGFLPDKVYTSKLARAKQTAEIILEELGAEVMIAELAALNERDFGEHDGKPYQSVL
                     TA"
     gene            complement(378..1643)
                     /locus_tag="VN778_05050"
     CDS             complement(378..1643)
                     /locus_tag="VN778_05050"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HXR50364.1"
                     /translation="MRRLSRLRRSSIYRYDQPVRPSRGREFMLFTLALVTVAILGMHF
                     YRPASHDKTASTPPRAAISTAASTPIKAGTSDSIVLPADAQALGIAAGSSLTGLSGQQ
                     LDSRLQAIAATGATWIRYDFDWSLIQPDSATSYKWQTYDNLVAASSKYHLRILGILDY
                     TPGWARMSDCGSSQCAPADPQAFAHFASAVANRYKGSGLHYWEVWNEPNNPTYWQPAP
                     DPAAYSRLLQASYRTLHSADSQAVVLTGGLSPQATTSNSYAPTDFLAALYKAKAGGSF
                     DAVGDHPYTYPLTPVSTGDNAWTEMAGDSNSLRALMVANGDSTKKIWITEFGAPTNGP
                     GQVATIANPNLDAGPHVVDENLQAKDLADALQRYQSYSWAGPLFYYSYQDAGTSTDTN
                     ENFFGLVRASGSHKPAYSVFYTAAHKINH"
     gene            1719..1916
                     /locus_tag="VN778_05055"
     CDS             1719..1916
                     /locus_tag="VN778_05055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50365.1"
                     /translation="MMHSFNFADPHIQLRFEALFAIGFMLGCLAVIEYAGPKRRAHWL
                     GLISALVGNGLFLAYTLVRLR"
     gene            1921..2619
                     /locus_tag="VN778_05060"
     CDS             1921..2619
                     /locus_tag="VN778_05060"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HXR50366.1"
                     /translation="MKRVALSTKLGHGVSRALHYYADWRIGATLAEKQCHKQSDSILL
                     TFDDYGDRRQITAILDVLAREEVKAVFFLESRWAEAHPELLALIQAAGHVIGNHTYSH
                     PNLLKLSDRDIETEIKKGLPSQWLRAPMGRYNARVRAIANRLGYQMCYWTIDSRDWKG
                     TSEATMRHTIRSELAPGAVILFHLHGRHTAAALPAIIRDIRATGYHLTAPAEDWAPQG
                     AGDLAGAYVFEAKA"
     gene            complement(2633..>4099)
                     /locus_tag="VN778_05065"
     CDS             complement(2633..>4099)
                     /locus_tag="VN778_05065"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HXR50367.1"
                     /translation="KFAFQLFAALISVVLYSTLRDYVPRRIAFLATFLCISFPVFLTD
                     ITMLNRQETALLCFALALQTGLDKRLKPATKSVLTGIFLVGMVWAHYSTSYVAVAILV
                     LAALMEIVWRAFEYRQRRRPSREAAPISSRLPAIISLPVTIGSLLILVAWGTVATQTS
                     NNVTQTVGGVIALVTGARPPATPAASNQAVSATQYATATTDTRPAFVRDYYADGQTAN
                     NQIKNEQPIIAAPSPLLQKHGISSQLLGKFYDLVRSAYSLFIEIGIVIGLVWWYMTRR
                     SAKLPRQYLLFGVAGLFVIAAQAVLPSTLINYGLTRVIQEALLVLALPIVLAWLWGMK
                     LIRIPRRLREPLLAVILAVFFLILSGFVSALSGGYKPVLGLSNTGLYYQAYYTHEDEV
                     SAAYWLGTQIPTGSHIVSDEFMRRKIIAYENIFAQTGIIPGAIPKDSYVVISHGDREF
                     NEVPAYDRSEVIYYSVPYQFLDGHKDLVYSTGNIVIYK"
BASE COUNT          935 a         1034 c         1187 g          943 t
ORIGIN      
        1 gccgtcagca ccgattggta cggtttgccg tcatgttcgc caaagtcacg ttcgttaagc
       61 gccgctagct ctgcaatcat cacctccgcg cccagttctt caagtatgat ttctgcagtc
      121 tgttttgccc gagcaagttt cgaagtatag actttgtcgg gtaaaaagcc ttccgctttt
      181 aaagctgcac ccttagcgcg ggctacctcg atgccctttt tggatagtgg gctgtcaagt
      241 tttgccccca ttaaaatacc ctgttcgttg gctaaggcaa gtgaatgacg aataactgtt
      301 aagctaggca tcacataaga ataaaataaa aaagccggcg tgtatagcgc tgaggtagca
      361 attctccata caccaagtta atgattaatt ttgtgcgcgg cagtgtagaa aacgctgtaa
      421 gccggcttgt ggctgcccga ggcacgcacc aggccgaaaa agttttcatt ggtatcggtg
      481 ctggtacccg cgtcttggta gctgtaataa aataaggggc ctgcccagct gtagctttga
      541 tagcgctgta aagcgtcagc taggtccttg gcttgcaggt tctcgtcgac gacatgcggg
      601 ccagcatcta agttcgggtt agctattgtg gccacctgcc ccggaccatt ggtgggcgca
      661 ccaaattcgg tgatccaaat tttttttgta ctatcaccat tagccaccat gagggctcgg
      721 agactattgg agtcgccggc catttcggtc caggcgttat cacccgtgct gactggcgtc
      781 aaaggatacg tgtacggatg atcgccaacc gcatcgaaag agccgccggc tttggcctta
      841 tagagcgcgg ccaggaaatc ggtgggcgcg taggagttac tggtggtggc ttgcggcgat
      901 aagccacccg ttaagacaac cgcttgggag tcagcactat gcagagtccg gtagctagct
      961 tgcagcaggc ggctgtaagc agccggatcg ggcgccggtt gccagtaggt cggattattc
     1021 ggttcgttcc atacttccca gtagtgtagc ccactgccct tatagcggtt ggctacggca
     1081 ctggcaaaat gggcaaatgc ttgggggtcg gctggagcgc attgcgagct accgcaatcc
     1141 gacatccggg cccatccggg ggtatagtca aggatgccca gtatgcgcag atggtatttc
     1201 gagctggccg ccaccagatt gtcataggtt tgccacttat aggaggtggc actgtcgggc
     1261 tggatgagac tccagtcaaa gtcgtagcgg atccaggtag cgccagttgc ggcaatggcc
     1321 tgcaggcggc tatcgagctg ctgtccggag aggccggtta gagagctgcc agcagcaatg
     1381 ccgagagcct gtgcatcggc gggaaggacg atggagtcgg aggtgcctgc cttgatcggg
     1441 gtgcttgcgg ctgtacttat cgcggcacgt ggtggcgtac tggccgtttt gtcgtgactg
     1501 gcgggccggt agaaatgcat gcctaagatt gccacggtga ccagggcgag agtgaacagc
     1561 atgaattccc gtccgcggct tggccgtacc ggttgatcgt agcggtagat gctgcttcgt
     1621 cttagccgac ttaaccggcg catggatctc cttaaatata ttgacaattc taactctaag
     1681 tattatagta tcataataaa gctacgctta ccagttatat gatgcacagt tttaattttg
     1741 ccgatcctca tatccagttg cgctttgaag cgttgtttgc cattggcttc atgcttggct
     1801 gcctggcggt tatcgaatat gccggtccca agcggcgggc acattggctg ggccttatca
     1861 gcgctctcgt cggcaacggc ctatttttgg cctacacgct cgtgagattg cggtaagact
     1921 atgaagcgcg ttgctctcag taccaaactt ggccacggcg tctcgcgagc cttacattat
     1981 tatgccgact ggcggattgg tgccaccttg gccgaaaaac agtgtcacaa acaaagcgat
     2041 tccatacttc tgacttttga cgactatggc gaccgacggc aaatcacggc gattttggat
     2101 gttttggctc gcgaggaggt caaagctgtg tttttcctcg aaagtcgctg ggcggaagcc
     2161 catccggagc tattggcatt aattcaagcc gccgggcatg tgatcggcaa tcatacctac
     2221 agtcacccga acctgcttaa attatctgac cgagatattg agacggaaat caaaaagggc
     2281 ctgccgagcc aatggctccg agcgccgatg ggccgttaca atgccagggt gcgggccatt
     2341 gctaaccggc ttggctacca gatgtgttac tggactattg atagccggga ctggaaaggt
     2401 acctctgagg cgaccatgcg ccacaccatc cgcagcgaac tggcgccagg tgccgtcatc
     2461 ttattccatc tccacggccg ccacactgct gctgccctcc cagccattat tcgcgatatt
     2521 cgtgccaccg gctatcactt gactgccccg gccgaggact gggcgccgca aggggccgga
     2581 gatctggctg gcgcttacgt cttcgaagcc aaagcctaac cttctaagct agctacttat
     2641 atatgacaat gttgcctgtc gaatagacaa ggtctttatg gccatctagg aactgatacg
     2701 gcacgctgta gtaaataacc tctgagcggt cataggccgg cacttcattg aattcacggt
     2761 cgccgtgact aataaccacg tagctgtctt tcggtatggc gccaggaatg atgccggtct
     2821 gggcgaaaat attctcgtaa gcgatgatct tacgacgcat aaattcgtcg ctgacaatat
     2881 gcgaaccagt cggaatttgc gtaccgagcc aataagcggc gcttacttcg tcttcatggg
     2941 tgtaataggc ctgataatac aagccggtat tactgaggcc gaggacgggt ttgtagccgc
     3001 cgctaagggc cgagacaaag cccgataaaa tcaagaagaa aactgccagg ataacggcta
     3061 gtaagggctc gcgtagacgc ctcggtattc gaatgagctt cataccccaa agccaggcca
     3121 gaacgatagg caaagccaat acgagtagtg cttcttggat cacgcgggta agaccgtagt
     3181 tgatgagggt cgatggcagc acggcctggg cggcaatgac aaataagccg gcgacgccga
     3241 acagtaagta ttgacgcggc agcttggcgc tgcgcctcgt catgtaccac cagacgaggc
     3301 cgataacaat gcctatttcg ataaatagac tgtaagcgct acgcactagg tcgtagaact
     3361 tgccgagaag ctgcgacgaa ataccgtgct tctgcagtaa ggggctcggt gccgcgatga
     3421 tcggctgttc gttttttatc tgattgttag ctgtttggcc gtcggcatag taatcccgga
     3481 caaatgcagg ccgagtatca gtggtagcgg tagcgtactg ggtggctgaa acggcttggt
     3541 tggaagcggc cggggtggct gggggccgcg cgccggtgac taaggctata acaccgccga
     3601 ctgtctgggt gacattattt gacgtttggg tagcgactgt tccccaggcg acgagaataa
     3661 gtaggctgcc aatggtaacc ggcaggctaa taatggctgg gaggcggctg gagatcggtg
     3721 ccgcttccct gctgggccgc cgccgctggc ggtattcgaa agcccgccag acaatttcca
     3781 tcagagcggc caggactaat atggctacgg caacgtagct ggtcgagtag tgtgcccaaa
     3841 ccatccccac cagaaagatg cctgtcaaca ccgatttggt ggctggcttg agccgcttat
     3901 ccaagcccgt ctgaagtgca agggcaaaac acagcagtgc tgtctcttga cggttgagca
     3961 tggtaatgtc ggtcaagaat accgggaaac tgatacataa aaaggtagcg agaaaagcga
     4021 tgcgacgagg tacgtaatcg cgcagcgtac tgtagaggac tacgctgatg agggcggcaa
     4081 aaagctggaa ggcgaattt
//
LOCUS       DATNGP010000196         2020 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_1213812, whole genome shotgun sequence.
ACCESSION   DATNGP010000196 DATNGP010000000
VERSION     DATNGP010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1213812"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..340)
                     /locus_tag="VN778_05070"
     CDS             complement(<1..340)
                     /locus_tag="VN778_05070"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HXR50368.1"
                     /translation="MAAQQHGLGRGFDALIPQNFDSSILLDEADRVQKLPIESLAPRT
                     DQPRQTFDETALEELADSIRAHGVLMPLVVTKVTDGTYRIIAGERRWRAAKIAGLKAL
                     PAIVRTLKELE"
     gene            complement(342..1115)
                     /locus_tag="VN778_05075"
     CDS             complement(342..1115)
                     /locus_tag="VN778_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003323489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HXR50369.1"
                     /translation="MSSIVAVLNQKGGVGKTTTAVNVATYLAREGQKVLLVDLDPQAN
                     STSGLGVQRAEGEAGTYEVMLGQTKAMDVVKPVFDRLHLMPTDARLAALEIELVDQTD
                     REKALRNALAGADDYDYVIIDCPPALSLLTINALTAADSLLIPVQAEYYALEGLSQLL
                     QVVQMVRSGLNPNLELLGVVMTMYDPRTTLSSQVKGEVERHFGDKVFDVVIPRNVRLA
                     EAPSHGKPIADYDKWSKGARAYKNLAKEVHKRVQKGKSK"
     gene            complement(1359..1901)
                     /locus_tag="VN778_05080"
     CDS             complement(1359..1901)
                     /locus_tag="VN778_05080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50370.1"
                     /translation="MVKKFLGRAREFADTNKTWVVSWVVIILSIFLTIHFWPKAPSPV
                     PSHIQQQAGFVIIYPDPRSYSYKNAGWNYTLKDGSVSFTVKKDGYSVAFNEQQTPLEY
                     QNDVASYNRFIGSLRPSANFNVPLGTVSITNFVTAGDYQVVGKTGILNSQNTLLLAHP
                     DTDLTDNQWRDLFESLQISN"
BASE COUNT          453 a          563 c          501 g          503 t
ORIGIN      
        1 gctccagctc ctttagcgtc cgcacgattg caggaagggc cttgaggccg gcaattttgg
       61 ccgcccgcca acggcgttca ccggcgataa tccgatatgt gccgtctgta actttcgtga
      121 caactagcgg catcaatacg ccgtgggcac ggatcgaatc ggccagctct tcgagtgcgg
      181 tttcgtcaaa tgtttggcgc ggctgatcgg ttcggggtgc aagcgattca atcggcaact
      241 tctgtacccg gtcggcttcg tcgagcaaga tagagctatc gaaattttgc gggataagcg
      301 cgtcaaaacc gcggcctaaa ccatgttgtt gcgcagccat attacttgga cttacctttc
      361 tgcacgcgct tatgaacctc tttggcgaga ttcttgtagg ctcgcgcacc tttgctccat
      421 ttatcataat cggcgattgg cttaccatgg ctcggagctt cggccaggcg gacattgcgc
      481 ggaataacga catcaaaaac tttgtcgcca aaatgacgct ccacttcacc ttttacctgg
      541 gacgacagcg tggtgcgcgg atcgtacatc gtcataacaa ccccgaggag ttcgagattg
      601 gggttcaagc ctgagcgtac catttggacg acttgcagca gctggcttaa accctccaga
      661 gcgtaatatt ccgcctgtac ggggatgagt aaactatctg ctgccgtcag agcgttgatc
      721 gtcagcaggc tcagcgctgg cgggcagtca atgatcacgt aatcgtagtc atctgcccca
      781 gccagagcgt tcctcagtgc tttttcgcgg tcagtctgat caactaactc aatctccaga
      841 gctgccagac gggcgtcggt gggcatcagg tgcaagcggt cgaacaccgg cttcacaacg
      901 tccattgctt tggtctggcc caacataacc tcgtaggttc cagcttcacc ttcagcacgc
      961 tgtacgccca aaccactcgt actgttggct tgcgggtcca ggtccaccag gagcactttt
     1021 tggccctcac gtgccaagta cgtcgcgaca ttaacagcgg tagttgtctt gccgacgcca
     1081 cctttttggt ttagaactgc aacgattgag ctcatctctg ttttataccc tctgctaccc
     1141 cttaagccta ccatttttgc gttcagccgt aaacttccgc gcattcgcca ttcacacgct
     1201 tcgccctccg tcattcccga catgagctta cccggcctag cctggcaaag ccagcctctg
     1261 ccagttatca cgagttacca gatcgggaat ccagtaataa aaggatcatt tttgactgga
     1321 ttcccatttt catgggaatg acgaaacatg gacgtaggct aattgctgat ttgtagtgat
     1381 tcgaacaggt cgcgccactg gttgtcggtt aggtcggtgt cggggtgtgc cagtaacagc
     1441 gtgttctgac tattaagaat cccggtcttg cctactacct gatagtcgcc agcagtgacg
     1501 aagttagtga tcgacactgt gccaagcggc acgttgaagt tggcgctcgg gcgcaggcta
     1561 ccgatgaatc gattgtacga cgcaacatcg ttctggtatt ccagcggcgt ctgctgctcg
     1621 ttgaaggcca ccgagtagcc gtcttttttc actgtaaatg ataccgagcc gtcttttaac
     1681 gtataattcc agccggcatt tttatatgaa taactgcgcg gatcagggta aatgattacg
     1741 aaaccagctt gctgctgaat gtggctcggc accgggctcg gcgccttcgg ccagaaatga
     1801 attgtcagga aaattgacag aataatcacc acccacgaca caacccacgt cttgttcgta
     1861 tccgcaaatt ccctagctcg ccccaaaaac ttcttaacca ttagcccaat tgtagcatcc
     1921 tagctaagcg ctcacgtctc gtacaatctg acgatcaaaa atctataagc caggcgtgaa
     1981 cacgacgact ggttctccgg cctatacaat cagtgacgac
//
LOCUS       DATNGP010000197         3442 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_4557940, whole genome shotgun sequence.
ACCESSION   DATNGP010000197 DATNGP010000000
VERSION     DATNGP010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3442
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4557940"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(43..243)
                     /gene="rpmC"
                     /locus_tag="VN778_05085"
     CDS             complement(43..243)
                     /gene="rpmC"
                     /locus_tag="VN778_05085"
                     /inference="COORDINATES: protein motif:HMM:TIGR00012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HXR50371.1"
                     /translation="MKVAEIRKMSTTDLTKESTKLREEITELRRRVHMGEVQNVRAIR
                     AKRKDLARMLTILGEALVKEAK"
     gene            complement(246..659)
                     /gene="rplP"
                     /locus_tag="VN778_05090"
     CDS             complement(246..659)
                     /gene="rplP"
                     /locus_tag="VN778_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015642164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HXR50372.1"
                     /translation="MLLPKRQKHRRVRKGRIRGVATSGNYIAFGDFALQAQGQERITS
                     RQIEAARQAMTRYIKRGGKIWIRIFPHTPVTRKPQDVKMGSGKGNPEFFVAKVRPGTI
                     MFEMQGVSEEVAREAMRLAAHKLPVKTKFLVREEA"
     gene            complement(659..1288)
                     /gene="rpsC"
                     /locus_tag="VN778_05095"
     CDS             complement(659..1288)
                     /gene="rpsC"
                     /locus_tag="VN778_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006890483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HXR50373.1"
                     /translation="MGQKVNPISMRLQVNKDWRSKWFADKRTYAKFLKDDLEVRRLIS
                     NKMGSRAAINKVEINRTPNLVTVTIATAKAGVVIGRGGASAAELKDAIEKIYGIPTRV
                     NIEEVKKPDLFAKLVAENIAHQLERRMSFRRAVKSAAAATMRAGAQGVRIEVAGRLNG
                     AEMSRREKEVAGSVPLHTLRADIDYAQVNARTTAGIIGVKVWIYKGEVQ"
     gene            complement(1288..1677)
                     /gene="rplV"
                     /locus_tag="VN778_05100"
     CDS             complement(1288..1677)
                     /gene="rplV"
                     /locus_tag="VN778_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015642167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HXR50374.1"
                     /translation="MAVKAVAKGVHMSPRKVAVVAALVRGRSVEDALTILDHTPRRSA
                     LAVKKVIMSAKANAAHNHNYKPATLQITEISVTPGPRIKRYRPAAHGRALPFQRKSSH
                     IRVIVDGEQREAKKPVAKTAVKTEEKK"
     gene            complement(1686..1958)
                     /gene="rpsS"
                     /locus_tag="VN778_05105"
     CDS             complement(1686..1958)
                     /gene="rpsS"
                     /locus_tag="VN778_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012475659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HXR50375.1"
                     /translation="MSRSLKKGPFIDPKLAKKVNALGDNDRTIIKTWARASTISPEFV
                     GKTIAVHNGKVHVPVFVTENMVGHKLGEFAPTRKFRSHGGKLAKGK"
     gene            complement(1955..2263)
                     /locus_tag="VN778_05110"
     CDS             complement(1955..2263)
                     /locus_tag="VN778_05110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50376.1"
                     /translation="MSERIYSITLPPRVMAEKAFSGDLTPMQEEQVKLGHIQGRDYAL
                     AAHQNGQEFNPMQVIAERFVSLATSGDDAEAAPGAEYFVVPFLLSAMAMNKEIVRVRV
                     "
     gene            complement(2268..3122)
                     /gene="rplB"
                     /locus_tag="VN778_05115"
     CDS             complement(2268..3122)
                     /gene="rplB"
                     /locus_tag="VN778_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018249353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HXR50377.1"
                     /translation="MAIKTYNPTTPGRRGMTTQDFDAITTKKPLRSLLVPKRRGNGRN
                     NQGRITTRHKGGGTKKFYRLVNFKLADGVTATIEHIEYDPNRSARIARIKEDNGTYHY
                     ILAAQGMKVGQKIASGEEAGIDNGNRLPLKNIPIGSTIHAIELKVGGGAQLVRSAGNS
                     AQLMGRDNGYAQIRLPSGEVRLINENCTASLGTIGNEQHQNIKIGKAGRNRHKGIRPS
                     VRGVVMNAADHPHGGGDGGRHGIGGGKNHGAAPKTPWGQKTLGYKTRRRKNTSQFIVR
                     TRHQGKRK"
     gene            complement(3281..3442)
                     /gene="rplW"
                     /locus_tag="VN778_05120"
                     /pseudo
     CDS             complement(3281..3442)
                     /gene="rplW"
                     /locus_tag="VN778_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011041720.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
BASE COUNT          695 a          929 c          873 g          945 t
ORIGIN      
        1 cagtcttgtt cgacgacacg gttccaataa ttgtcttagc cattacttgg cctcctttac
       61 tagcgcttca ccgagaatgg tcaacatgcg agccagatcc ttacgtttgg cacgaatagc
      121 ccggacattt tgaacttcgc ccatgtggac acggcggcgg agctcggtga tttcttcgcg
      181 tagcttggtg ctttccttgg tcaggtcagt cgtgctcatt ttgcgaatct cagcaacttt
      241 cattatcacg cctcctcccg gaccaggaat ttggtcttga ctggtagctt gtgagcggcc
      301 aaacgcatag cttcacgagc tacttcttcg ctgacgccct gcatttcgaa catgatcgta
      361 ccgggacgta ctttggccac gaaaaattca ggattacctt taccactgcc catcttcaca
      421 tcttgcggtt tacgggtaac tggggtgtgg ggaaagatac ggatccagat tttaccaccg
      481 cgcttgatgt aacgggtcat ggcttgacga gcggcctcga tctgacgcga agtaatgcgc
      541 tcttggcctt gagcctgcaa ggcaaaatca ccaaaggcaa tgtagttgcc cgaggtggct
      601 acgccacgga tccgaccctt gcgcactcga cggtgttttt ggcgtttcgg cagtaacatt
      661 actgaacctc tcccttataa atccagacct tcacgccgat gatgccggca gttgtccgcg
      721 cattaacttg ggcataatca atatcggcac gtagtgtgtg taatggcacg ctaccagcaa
      781 cttctttctc acggcgcgac atttcagcgc cattcaagcg tccggcaact tcaatgcgca
      841 ctccttgagc gcccgctcgc atggtggcag cggcggctga tttaacagcg cggcggaagc
      901 tcatgcggcg ctccagctgg tgtgcaatat tttcagcaac cagttttgcg aacagatcag
      961 gtttcttgac ttcttcgatg ttaacacgcg tcgggatgcc gtaaatcttc tcgatcgcat
     1021 ccttaagctc cgcggcactg gcaccaccac gaccgataac cacaccggcc ttggcagttg
     1081 cgattgtaac cgtcacgagg ttaggcgtgc gattaatttc aaccttgttg atagccgcgc
     1141 ggctacccat cttgttgcta atcaaacggc gaacttcaag gtcgtctttc aaaaacttgg
     1201 cgtaggtgcg cttgtcagcg aaccatttgc tgcgccagtc tttattgacc tggagccgca
     1261 ttgaaatcgg attaactttc tggcccatta tttcttctcc tcagtcttaa cggccgtctt
     1321 ggcaactggc tttttagctt cacgctgttc gccatcgacg ataacgcgaa tatgacttga
     1381 tttacgctgg aagggtaagg cacgaccatg cgctgccggt cgataacgct taatacgcgg
     1441 accaggcgtc acgctgattt cagtgatctg cagtgttgcc ggcttgtaat tatggttgtg
     1501 ggcagcattc gccttagcac tcataatgac cttcttgaca gccaaagcgc tgcgacgcgg
     1561 cgtatgatcc aaaatagtca gtgcatcttc gacgctgcgg ccgcgtacta gagcagctac
     1621 cacggcgacc ttgcgcggac tcatatgtac tcccttagcg actgctttaa ctgccataat
     1681 tatccttatt tacctttcgc gagcttcccg ccatgactgc ggaacttacg agttggcgca
     1741 aactcaccaa gcttgtgccc aaccatattc tctgtaacaa acaccgggac gtgaacttta
     1801 ccgttatgca ccgcaattgt cttcccaaca aactctgggc taatggtgct agcccgtgcc
     1861 caagtcttaa taatagtccg gtcattatca cctaaagcat tgaccttctt agccaatttc
     1921 ggatcaataa acggcccttt ctttagtgaa cggctcatac ccgaaccctc acaatctctt
     1981 tattcattgc cattgcgctt agtaaaaatg gcacgacaaa atattcggcg cccggtgctg
     2041 cctcggcatc atctccagat gtagccaaac ttacgaacct ttcagcgatg acttgcatcg
     2101 gattgaattc ttggccattt tgatgagcgg caagtgcgta gtcacggcct tgaatgtgtc
     2161 ccaatttaac ctgttcctct tgcattggag ttagatcacc gctgaaggcc ttttcagcca
     2221 taacgcgcgg gggcaaggta attgaatata ttctttctga catcttctta tttcctcttt
     2281 ccttggtgac gtgtgcggac gatgaattgg ctggtgttct tgcggcggcg ggttttgtag
     2341 ccgagggttt tctggcccca tggggtcttg ggtgcggcac cgtggttttt gccaccgccg
     2401 atgccgtgac ggccgccgtc accaccaccg tgcgggtgat cggcagcatt catgacaaca
     2461 ccgcggaccg atgggcggat gcctttgtgg cggtttcggc cagccttacc gatcttgata
     2521 ttttgatgct gctcgttgcc gatagtgcca agtgaggcag tgcagttttc gttgattaag
     2581 cgaacttcgc cgcttggcaa gcgaatctga gcgtagccat tatcgcggcc catgagttga
     2641 gcgctgttgc cggcgctgcg gacaagctgg gcaccgccac cgacctttag ctcgatcgcg
     2701 tggatggtgc tgccgattgg gatattcttc aggggtaagc gattgccgtt atcgataccg
     2761 gcttcttcac cactagcaat tttctggccg accttcatgc cttgggcagc caaaatgtag
     2821 tggtaggtgc cgttgtcttc tttgatgcga gcaatgcggg cgctgcggtt cgggtcgtat
     2881 tcgatgtgct cgatagtggc ggtaacgccg tcggccagct taaagttaac caggcgatag
     2941 aattttttcg taccaccacc tttgtgacgg gtggtgatgc gcccctggtt attacggcca
     3001 ttgccgcgcc ttttaggcac cagcagagaa cgcagtggct tcttggtagt gatagcatca
     3061 aagtcctgcg tggtcatgcc gcggcggccg ggagtggtcg gattgtaagt cttaatcgcc
     3121 attacttatc ctcctgggct tttttgcggc gcaaaccact aagacgaggc tttttctcct
     3181 ctttggcggt ttgcttagca actgctttgt caaccttttc ctgtgtggct ttttcttgct
     3241 cttcagcctc ttcgatggcc gcgaagaatg gtaagccttg gccctcggct aacgtgatgt
     3301 aagctttctt gatgtcggaa cgtttgcctt cagcgttttg gtagcgcttg ccggtcaagc
     3361 tgatgactcg cttcgatttg ccagagatgt ttgtcgtatt caccttgaca accttaacct
     3421 caaactgctc ttcgatagcc cg
//
LOCUS       DATNGP010000198         4629 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_467467, whole genome shotgun sequence.
ACCESSION   DATNGP010000198 DATNGP010000000
VERSION     DATNGP010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4629
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_467467"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            53..129
                     /locus_tag="VN778_05125"
     tRNA            53..129
                     /locus_tag="VN778_05125"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:87..89,aa:Lys,seq:ttt)
     gene            complement(517..843)
                     /locus_tag="VN778_05130"
     CDS             complement(517..843)
                     /locus_tag="VN778_05130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50378.1"
                     /translation="MLASDNPKKQQRILYFSDNSGINSGNITSPSMTQFSLRLLTSTR
                     ISFNVGWNNLPLSKIEAPSKLGIPRIWLRLYKNLMKKRALAEPGLASQQIGFYQLWAH
                     LQLDKS"
     gene            842..1057
                     /locus_tag="VN778_05135"
     CDS             842..1057
                     /locus_tag="VN778_05135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50379.1"
                     /translation="MEEILKYGGYFNWPQWKLENGLEDEVKDLVERTVSRSSIIKQEA
                     EKYKLPYFDLATNYSQTSKAALSRLLS"
     gene            1188..1724
                     /locus_tag="VN778_05140"
     CDS             1188..1724
                     /locus_tag="VN778_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009198130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HXR50380.1"
                     /translation="MQTSYGILGVLSTEPNYGYELKRLYDQYFGQKKPLAYGQVYATL
                     ARLKRDGKIVAEGHEQSGGPARTKYAITNEGQRDLERWLATPETIQSELRSTAFAKIV
                     AAILLDKDPHRYLDIQRSSHIARMRELTKIRRHADLATALRADYEIFHLEADLRWIDL
                     TFSRLSVLTQEIANEQNR"
     gene            1708..2400
                     /locus_tag="VN778_05145"
     CDS             1708..2400
                     /locus_tag="VN778_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018561396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXR50381.1"
                     /translation="MNKTDNILTGQQIVKTFGQTKALDGADIEIGRGERLAIMGASGS
                     GKSTLLHCLAGITMPDSGEVWFDGRRLDTLDDNTKTKLRRTAFGFIFQFGQLVPELTA
                     VDNVALPLLLNNVPRGEAYKRAVEWLQHLGIDKVAGQQIGTLSGGQSQRVAIARALVV
                     NPKVLFADEPTGSLDSLNSEKVMELLVDIARHQAVTLVVITHDSRVAAYCEREVIVRD
                     GKVNQYAQKAGA"
     gene            2397..2939
                     /locus_tag="VN778_05150"
     CDS             2397..2939
                     /locus_tag="VN778_05150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50382.1"
                     /translation="MIFRLGLRLAIRGNKEGTIRQIIITLSTAIGVCLLLFTLSGLTV
                     LNAGFSKPCWSCAGKAQPGQSNDGLLWSYNTDTYKGSNITSVHIAALGAKAPGIPGVA
                     QLPRPGSYVASPAMRRLLQTAPQAEFGARYSDFTYAGTIGNTALSSGRELVIITAETP
                     RALVASGNAVSKTYHVGPNA"
     gene            2940..3743
                     /locus_tag="VN778_05155"
     CDS             2940..3743
                     /locus_tag="VN778_05155"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HXR50383.1"
                     /translation="MSGTRQLVDVLFLILSIGLLFPILVLIGNAARLSASRREERLAV
                     FRLVGATSRQVNVLAVIDALVSTLVGVAIGTALFVVLKQQLIDHRAATLAFFATDFTL
                     QVTGYAAIIIGVPLLSAVAGIWALRKVNISPLGVVRRISAKPPSFKRALPFFIGLAMF
                     LSSTVAVSHYANTSLPSLFSLMLIFGFLLMIVGIVFAGPWITLMTARFIARHSSKSYT
                     LLASRCLASYPTAAFRSVSGVILAVFVGTCLAGAAPALYSSHGPSEAPP"
     gene            3794..4624
                     /locus_tag="VN778_05160"
     CDS             3794..4624
                     /locus_tag="VN778_05160"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXR50384.1"
                     /translation="MTTDKAHTLRTNLEAIQGVRAYPVYAEASSLSDPPASVDAKGIV
                     SCQALAALAAFGNCPPGSQVATMQVLDVLLKAQLDTRTTHPLPEQRTLSAYSSNHVLA
                     FFVQARDASALERARTHLYLHAAQASPPRTYGEQFNDSLTQSKRLRQIVNISMVVTLI
                     IAGSSLAVSTLGSLFERRRTFTLLRLSGTSMTTLRQVVIMETVAPLLICALLSIGTGY
                     VIAYAMTNAIDPGPMKALSLPTNFYITLGMGLPLSIIIICATLPLLRRITQSENARYE
                     "
BASE COUNT         1144 a         1161 c         1146 g         1178 t
ORIGIN      
        1 tccccaaaaa tatcgataat ataagaagtt aaattcgccc gtacgccagt atgggcccat
       61 agctcagttg gttagagcag cggcctttta agccgcgtgt cgtgggttcg aatcccactg
      121 ggccctccac tcatgcgtat aatgatctta gtgcggcgag atcattttta ttcgattgtt
      181 tgcaatatta tcgttatcta ataatatgat catatggaag aagcaagaaa acagctgcct
      241 gaagagttaa ctgtacccca agaagtaaag gatcagttaa gagagcgaag tcttttgccg
      301 acaatgtggt atgttggtcc acttgctgtc cgtggacaag cagctattac accacctcga
      361 aggcgagttc gtagacctcc agggccctca gccgaaatcg ataattagca tactcgtcgt
      421 gagctcagtt acaattttgt cacacacggc cctccaagta tttcgtacaa gacttagact
      481 tctgtaaaag gaagtctttt ggttgctata cttattttat gatttatcta attggaggtg
      541 cgcccagagt tggtaaaagc caatttgctg cgaagctaat ccaggctcag ccaatgccct
      601 ctttttcatt agatttttat ataatcttag ccaaatacgt ggtattccta attttgaagg
      661 tgcctcaatt ttagacaaag gcaggttatt ccacccgaca ttaaaagaga ttctcgttga
      721 tgttaataag cgcagtgaaa actgcgtcat tgacggtgaa gttatattac cagaatttat
      781 acctgagtta tccgagaaat ataatatacg ctgttgcttc ttaggattat cagaagcaag
      841 catggaagaa attctaaagt atggtggcta ttttaactgg ccacagtgga agctagaaaa
      901 tggtcttgaa gatgaggtca aagatttagt tgagcgaaca gtcagcagaa gctcaatcat
      961 caaacaagaa gctgagaaat ataagttacc atattttgac ctagcaacta attacagcca
     1021 aacaagtaaa gctgctttga gccgcttgct ttcttgattt cggtttcaat agacatcacg
     1081 atggccctcc acgaaaaaga cctgccgaaa cgtaggtctt ttttcgtgtc tctcaaatga
     1141 aaatagcagc attgtattta actatacatt gagtgtatag cgtacatatg cagacatcat
     1201 atggcattct gggggtacta agcacggagc cgaactacgg ttacgagctc aagcgtttat
     1261 acgatcagta tttcggtcaa aaaaagccct tggcttacgg tcaagtttat gcgactttag
     1321 cgcgcctaaa gcgcgacggc aagatagtcg ctgagggtca cgagcaatca ggcgggcccg
     1381 cgcgcaccaa atatgccatc actaacgaag gccaacgcga tttagaacgt tggctggcta
     1441 cacccgaaac aattcagtcg gaactgcgct ctaccgcctt cgccaaaatc gtagcggcca
     1501 ttttactcga caaagacccc catcgttact tggacataca aaggtcgtcg catattgcgc
     1561 ggatgcgtga actaaccaaa atccgccgcc acgcagattt ggccacagcg ctccgagccg
     1621 attacgagat cttccacctg gaggctgatc tacgttggat tgatttaacc ttttcgcgtt
     1681 taagcgtgct aactcaggag atagccaatg aacaaaacag ataatatact tactggccag
     1741 caaatagtca aaacctttgg ccaaactaag gcgcttgatg gcgctgacat tgaaatcggt
     1801 cgtggcgaaa ggctagcgat aatgggtgcc agcggttctg gcaagtcaac cttgttacac
     1861 tgcctggctg gaattacgat gcccgattca ggagaggtct ggtttgacgg tcggcggctg
     1921 gatacgcttg atgacaacac caaaactaaa ttacgccgca cggcatttgg ttttattttt
     1981 caattcgggc agctggtgcc cgagctgacc gcggtagata atgtagcttt gcctttactg
     2041 cttaacaatg tgccaagagg cgaagcctac aaaagagcgg tggaatggct gcaacatttg
     2101 ggcatcgaca aagtggctgg ccaacaaata ggtacgctat ctggcggcca gtcgcaacgc
     2161 gtggctattg cgcgcgcctt agtcgttaac cctaaggtgt tatttgctga cgagccgact
     2221 ggctcgctcg attcgctcaa tagcgaaaag gtcatggagc ttttagttga tatagcgcgc
     2281 caccaagcag tcaccctggt agttattact catgattcac gggtagcagc ttattgtgag
     2341 cgggaagtaa tcgtccgaga cggcaaggtt aatcaatacg cccagaaggc aggcgcatga
     2401 ttttccgtct cggtctgcga ctggctatcc gtggcaacaa agaaggcacg attcggcaaa
     2461 taattattac gctgtcgacg gcaatcggtg tttgcttgct gctctttacg ctatccggac
     2521 ttacagtgct gaatgccggc tttagcaagc cctgctggag ttgtgccggc aaagcgcagc
     2581 cgggccagtc gaatgatggt ctattgtggt catacaatac cgatacctac aaaggctcga
     2641 atatcacgag cgtgcatatt gctgcccttg gtgccaaggc ccctggcata ccaggcgtag
     2701 cgcaattgcc gagaccaggt agctatgtgg cttcgccggc catgcggcga cttttgcaaa
     2761 cggcgcccca ggcagaattt ggcgcgcgct atagcgactt tacatacgcc ggcactattg
     2821 gcaacacagc gctcagcagc ggccgggagc tcgtcattat taccgccgaa accccgcgtg
     2881 cattagtcgc gagcggcaac gccgtcagca agacgtatca tgtagggcca aacgcctagg
     2941 tgagcggcac caggcagtta gtagatgtac tattcctgat actgtcaatt ggcctcctct
     3001 ttcctatctt ggtccttata ggcaacgcag cacgtttatc ggccagccgg cgcgaggaac
     3061 ggctcgcagt ctttcgactg gttggagcta cttcgaggca agttaatgtg ctggcggtta
     3121 ttgatgctct agtaagtacg cttgttggcg tggcaattgg cacagcgctt tttgtggttc
     3181 tcaagcagca gctgattgac caccgagcag ccacactggc attctttgca acggatttca
     3241 ctctgcaagt aacggggtac gccgcaatta ttatcggcgt gccgctactc tcggcggtcg
     3301 ccggcatatg ggcgctgcgc aaagttaaca tttcgccgct tggcgttgtc aggcgtatct
     3361 cggccaaacc accaagtttt aaacgagcgc tgccgttttt tatcggtctg gcgatgtttt
     3421 tgtccagtac ggtggccgtg agtcattatg ccaatactag tctgccgtcg cttttctcgt
     3481 taatgctcat cttcggcttt ttgcttatga tcgtcggtat tgtctttgcg gggccatgga
     3541 ttacactcat gactgctcga tttattgcgc gccactcaag caaaagctac accttgttag
     3601 cttctcggtg cttggctagt tatccgacgg ctgcttttcg atcggtcagc ggtgtcatct
     3661 tagccgtgtt cgtggggacg tgcctggcag gtgcggcgcc cgctctttac agtagtcatg
     3721 gaccctcaga agccccgccc tgaagtacac catccccctg gctttcttcg attttgacgg
     3781 taaggcagct ggcatgacaa ccgataaagc ccacactctg cgcactaatc tggaagccat
     3841 ccagggcgta cgggcgtatc ccgtctacgc tgaagccagc agcttatctg acccgcctgc
     3901 ttcggttgat gccaaaggta tcgtgagctg ccaagcgctt gccgccttag cagctttcgg
     3961 caactgcccg cccggtagcc aggtagctac tatgcaggtg cttgatgtct tactaaaagc
     4021 ccaacttgac acacggacca cgcatcctct tcctgagcaa agaacgctta gcgcttacag
     4081 tagcaaccac gtgctggcat tctttgtgca agcacgagat gcttcggcgt tggaacgcgc
     4141 ccgcacccat ctgtacttac atgccgctca agcttcaccg cctcgaacgt acggcgaaca
     4201 attcaacgat agcttgaccc aatccaaacg attgcgacag attgttaata taagtatggt
     4261 ggtgacgctc attatcgcgg ggagtagtct agcagtatcc acgctaggca gcttatttga
     4321 gcgtagacga acttttacct tattgcgcct cagcggcaca agcatgacca cgctcaggca
     4381 agtggtgata atggaaactg tagctcccct gctcatctgc gctttactgt cgataggcac
     4441 tggctacgtc attgcctatg ccatgactaa tgccatcgat ccgggcccca tgaaggcgct
     4501 tagtttgcca accaacttct atattacgct ggggatggga ttgccactgt ccattatcat
     4561 catttgcgct acattacccc tactgcggcg aatcacgcag agtgaaaacg cccgctacga
     4621 ataaggtgc
//
LOCUS       DATNGP010000199         8047 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_2404102, whole genome shotgun sequence.
ACCESSION   DATNGP010000199 DATNGP010000000
VERSION     DATNGP010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 8047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8047
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2404102"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..215
                     /locus_tag="VN778_05165"
     CDS             <1..215
                     /locus_tag="VN778_05165"
                     /inference="COORDINATES: protein motif:HMM:NF013370.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HXR50385.1"
                     /translation="HITSASHPFFTGEERILDIEGRVDKFKARAAAGQAAKEKRAQAA
                     QKASKRQAAKVEKAEAKAENTTKPSK"
     gene            333..1388
                     /gene="prfA"
                     /locus_tag="VN778_05170"
     CDS             333..1388
                     /gene="prfA"
                     /locus_tag="VN778_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HXR50386.1"
                     /translation="MDAKEQTLRQELAELESKLQNPAIFSDKNYPKLAKRKAQLDEVV
                     DLFDTTALLKKRRAEAEELLTSPDQELKALAALEIEASDAGIREQEEKLRELLTPQDP
                     NNDRDVIMEIRAAAGGDESSLFGGELYRMYSRWAENHGYKIELLSESPNEVGGFKEIT
                     FAVRGDQAYKNLKFEAGVHRVQRVPVTESQGRVHTSTVTVAVLPEAEETDVEIDPNDL
                     RIDVFRSGGHGGQSVNTTDSAVRITHLPTGIVVTNQDEKSQIKNKAKAMGILRSRLLQ
                     MKIDEEQAQLSDARRKLIGSGDRSEKIRTYNFPQDRITDHRIGYSRSNIPGALQGDID
                     DLVDSLQAAEHELLSQE"
     gene            complement(1396..1974)
                     /locus_tag="VN778_05175"
     CDS             complement(1396..1974)
                     /locus_tag="VN778_05175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50387.1"
                     /translation="MERNHSMPELAARFNMSVEQIQREIDFKNKITAIEQFLPGVSFQ
                     PLEEVIVNADIAYGALDDAGRKLNLDVASAKISANGYDHFEDKGIAFSTRIVKFNKTG
                     GGTDYYFAGIFAEKQPYLDGHYLPLGIIVEEGKITSLASFYYEEGMVQSLEEVFFDVH
                     LDDTADTPLHHTIVNEFLQGVTVNLSERLPRQ"
     gene            2041..2856
                     /locus_tag="VN778_05180"
     CDS             2041..2856
                     /locus_tag="VN778_05180"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HemK/PrmC family methyltransferase"
                     /protein_id="HXR50388.1"
                     /translation="MNVQNMLQQSIRKLEKASIGTARLDVLILLEDATGKDRAWLLAH
                     PEFELNDKQVKVIKNVLKRRAAHEPLAYIRGHTEFYGREFLLNQDVLEPRPESETMID
                     LLKKIMQPDRKQYIADVGTGSGALGITAQLELPDGLVELLDIDQKALKVAKMNVDKFT
                     LNISVKQSDLLAKASPKQDILLCNLPYVPDGFQINPAAMREPRIAIFGGPDGLDVYRK
                     LFNQVEELADEPLYILTEALPVQHELLRDIAESHDYTLLTSEDFIQVFQLKTS"
     gene            complement(2849..4147)
                     /locus_tag="VN778_05185"
     CDS             complement(2849..4147)
                     /locus_tag="VN778_05185"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HXR50389.1"
                     /translation="MSSEDKSKNPEVYSVSSGSRRPPRTVSFAVPEMPRIAKPDLGRL
                     RQGGAGLVIGIVLVAMLGGFAGAWVENHANNTGVISGSLSGQKKIVTSTSQLLSQIAK
                     TVGPSMVSVNVNITSSSSSQDGFGLFGFSQPEQEQAAGTGIVIASDGLIITNRHVVPA
                     GTTNVSVTLSDGTELKNVSVVGRTSDNDNLDIAFLKINDIKGHKLTPAVLGDSSTVQV
                     GDDVVAIGNALGQFQNTVTSGIISGYGRSVQAGDSSGSGSSENLDDLFQTDAAINEGN
                     SGGPLVNLNGQVIGINTAIAGDAQNIGFAIPVNDVKGLVNQVVKTGKFARPYLGVRYV
                     PLTADVANEYDLSVQNGAFVAPSADPTSPSVVSGSPADQAGIKENDVITQVNGTNIDQ
                     THSLTSLLDQHQPGDKVTLTVLRDNHTQHIDVKLGSAPSS"
     gene            complement(4159..4911)
                     /locus_tag="VN778_05190"
     CDS             complement(4159..4911)
                     /locus_tag="VN778_05190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50390.1"
                     /translation="MRPLLQRLKPTSGVSHFLHLILLVILPAAVFILVRLNFVQLALS
                     IIVLSKWRMFAVRPRFWAANVRANAVDLMVGVSIVLFTTHASGQVWLQLLLAVLYAVW
                     LIAIKPATGLPMITAQAFIGQLTALMALYLTWTNGPVYGLTLITGLICFLAARHYFDA
                     FEETYARMLSYIWGYFGAALAWLLSHWLLYYHGLAQPTLLLSALGYGLAVLYYLDHND
                     KLSRGMRRQFIFIMLAIVLVVLAYSDWGNKVV"
     gene            complement(4877..6268)
                     /locus_tag="VN778_05195"
     CDS             complement(4877..6268)
                     /locus_tag="VN778_05195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HXR50391.1"
                     /translation="MRRIDSLLAHGRWATARRWLFRGMAFALILMMVGGGLFFTQGYL
                     KLHKAFKGGSKTAAALQANVNPELLKGEGDGRVNILLMGRGGGNHDGPDLTDTMMLDS
                     IDPVNHTQTLISIPRDLWVDVPDAGAMKLNAAWETGEFRYLHKVRPGSTDPQAIQAGF
                     DLADQTVESVLGVTIHYNMLVDFQAFQQAVDTVGGVSVNVPTDLVDPTMAWENHGNSV
                     LAKAGPQTFDGKHALIYVRSRETTSDFARAQRQRAVLLALKTKVETLGTLSNPLKISG
                     LLSSFGNNVATDFSIKDATRAYSIVKNISDVDTQSIGLADPPNNYIKTGTMAGQSIDL
                     PAAGLFNYTDIQSFVRSQLKDPYILKENAKVLVLNGTNVPGLASKEATTLKAYGYNVV
                     GTANATTNNWAQTTLIDLTNGKDKYTKHYLEQRFNSTALTSLPDSTIQTNGADFVIIV
                     GSDATTPTTPQAN"
     gene            6566..7267
                     /gene="lepB"
                     /locus_tag="VN778_05200"
     CDS             6566..7267
                     /gene="lepB"
                     /locus_tag="VN778_05200"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HXR50392.1"
                     /translation="MDNDIKSPPEGPALPSKPSAPRAPVTPPAPVPKQGRIMPTKGTS
                     AWRELFSTVGILLTALAVALLIIAFVFRSYQVDGPSMESTLQNGDKLIIWKVPRSWAS
                     VTRHAYIPDRGDVIVFTESGLSQFGQTDTKQLIKRVIGLPGDRVVVANGIYTIYNKSH
                     PQGFDPDKSLPYGKNIPTTSGNVDVTLGKNQLFVSGDNRPDSLDSRAFGPINASQVVG
                     KLVLRVFPINKAEVF"
     gene            7280..>8047
                     /locus_tag="VN778_05205"
     CDS             7280..>8047
                     /locus_tag="VN778_05205"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HXR50393.1"
                     /translation="MNQTPKRGLGRGFESLLPADFNKTALLTPEDRIEKIPVADLVPN
                     PHQPRRHFDEKALQELAASIKRHGVVQPLVVTAVSAGKYTLIAGERRWRASRLAGLQT
                     VPAIVRTTKEIERLEIALIENVQRVDLSPLEQAASIQGLHEQFSLSYEEIAKRLGKAP
                     STISNSVRLLDLPEAARDALAARQISEGHARAILALRGDHERQTYLLKTIIGQGWSVR
                     QAERFVLSVKQGVKETARAHARTELETPATKKLSKQLG"
BASE COUNT         1994 a         2234 c         2015 g         1804 t
ORIGIN      
        1 tccacatcac ctccgcctca caccccttct ttaccggcga agagcgcatt ctcgatatcg
       61 aaggccgcgt cgataagttc aaggcccgcg ccgctgccgg ccaagccgcc aaagaaaagc
      121 gcgcccaagc tgcccaaaaa gcttccaaac gccaagccgc caaagtcgaa aaggctgaag
      181 ctaaagccga aaacaccacc aagccgagca aatagctcct ggccgttgat gttcacacta
      241 tgccgcaaga gtagtgtccc cattgtgtgc ccatagtatg tggacacgac ttctgactaa
      301 ctgtttaaaa acaagtatac ttaagatcga gtatggatgc caaagaacaa actttacgtc
      361 aagaattagc cgagctggag agcaaactcc agaatccggc tattttcagc gacaaaaatt
      421 atccgaaact ggccaagcgc aaagcacagc tagatgaggt cgtggacctc ttcgacacaa
      481 cggctttact gaaaaaacgc cgagccgagg ccgaagagtt actgaccagc cctgaccagg
      541 agttaaaggc gcttgctgct ttagagattg aagcttccga tgccggtatt cgcgagcaag
      601 aggaaaaact gcgggagttg ctaacgcctc aagacccgaa taacgaccgc gatgtcatta
      661 tggaaatccg cgccgccgcc ggcggtgatg agtccagttt gttcggcggc gaactttaca
      721 ggatgtacag ccgctgggca gaaaatcatg gctacaagat cgagctcttg agtgaatcac
      781 ccaatgaagt tggcggcttc aaagaaatta cctttgccgt gcgcggcgac caagcatata
      841 agaacttgaa attcgaagcc ggcgtccacc gcgtgcagcg cgtgcccgtc accgaaagcc
      901 agggtcgcgt acacacctct accgtcaccg tcgcagtgct gcccgaagcc gaagaaacag
      961 atgttgagat cgatccgaat gatctgcgta tcgacgtctt tcgcagcggt ggccacggcg
     1021 gtcagtcagt caacaccacc gactccgctg tgcgtatcac tcatttgccg actggtatcg
     1081 ttgtgaccaa tcaggacgaa aagtcacaga tcaagaacaa agctaaggcc atgggcattt
     1141 tgcgctctcg cctgctccaa atgaaaatcg acgaagagca agcccagctg tccgatgccc
     1201 gccgcaaact catcggctcc ggtgatcgct ccgaaaaaat ccgtacctac aacttccccc
     1261 aagaccgcat caccgatcac cgcatcggct actcccgctc caatattcca ggcgcgctgc
     1321 aaggtgacat cgacgatctt gtcgatagcc tccaagctgc cgaacacgaa ctcttaagtc
     1381 aagagtgaac agtccctact gccgaggaag ccgttccgat aaattgacag ttacgccttg
     1441 caaaaactca ttaactatag tgtggtggag tggagtgtcg gccgtgtcat caagatgtac
     1501 atcaaaaaac acttcttcaa gactctgcac cattccctct tcgtaataaa atgaagctaa
     1561 ggaggtaatt tttccctctt ctacaatgat ccctagcggt aaatagtgcc cgtcaagata
     1621 gggttgcttc tctgcgaaaa taccagcaaa gtagtagtcg gtcccgcccc ctgtcttatt
     1681 aaattttaca attcgcgtgc tgaaagctat gcctttatcc tcaaaatggt catagccgtt
     1741 cgcggatatt ttcgcactcg caacatctag gttaagcttt ctacctgcat catctagagc
     1801 accgtatgct atatcggcat taacaattac ctcttcaagc ggctgaaatg acacccccgg
     1861 gaggaactgt tcaatagctg taattttatt tttgaagtca atttctctct gtatctgctc
     1921 tactgacata ttaaatcgag cagctagttc tggcatcgag tggtttctct ccatgatttt
     1981 tatgattgta aatattttat gcaatttgtc aactttttaa gctttgctac acttaacttt
     2041 atgaatgtgc aaaatatgct gcagcaatct atacgcaagc tcgagaaagc cagtatcggc
     2101 acggcacgcc ttgatgtatt aattctcttg gaggatgcga ccggcaaaga tcgcgcctgg
     2161 ttattagctc atccggaatt tgagttaaac gacaagcaag ttaaggtaat caaaaatgtg
     2221 ctcaagcggc gagcagctca cgagccatta gcctacattc gcggacacac agaattctat
     2281 ggtcgcgaat ttttgctcaa tcaggacgtg ttggagcccc gccccgagtc ggaaacgatg
     2341 atagatttgc ttaaaaagat tatgcaacct gaccgtaaac agtacatcgc agacgtcggt
     2401 acaggtagtg gagcactcgg tatcacggct caactggagc tgcccgatgg ccttgtagag
     2461 ctactagaca tcgaccaaaa ggcccttaaa gtggccaaaa tgaatgtaga taaatttaca
     2521 ttaaacataa gcgttaagca aagtgacctt ctagcaaagg ctagtcctaa acaagacatt
     2581 ttgctctgca atctgcccta tgtaccagat ggttttcaga taaatcccgc agctatgcgg
     2641 gagccaagga ttgcgatttt tggcggacct gacggccttg acgtataccg aaaactcttc
     2701 aatcaagtcg aagaactagc cgacgaaccg ttgtatattt taactgaggc cctcccggtg
     2761 caacacgaac tgcttagaga tatcgccgag tcgcatgact atactctact gaccagtgag
     2821 gacttcatcc aagttttcca gctaaagact agctagacgg tgcactgcca agtttgacgt
     2881 caatatgctg cgtatggttg tcccgcagca cggtcaaagt caccttatcc cctggttgat
     2941 gctgatccaa aaggctggtt aggctatgtg tctggtcgat attagtgccg ttaacttgag
     3001 taataacatc gttttccttg atacccgctt ggtcggctgg gctaccggaa accactgacg
     3061 ggctggtcgg atcagcactg ggtgcgacaa aggcgccgtt ttgaacactt aagtcgtact
     3121 cgttggcgac atcggctgtc agcggcacgt aacgcacacc gaggtacggt cgggcgaact
     3181 tgccggtttt gaccacttga ttgaccaagc ccttgacatc gttgaccggt atggcaaagc
     3241 cgatgttttg agcgtcaccg gcgatggcgg tgttgatgcc aattacttgc ccattcaggt
     3301 tgaccagcgg ccccccggag ttaccttcgt tgatagcggc atcggtctgg aacaggtcgt
     3361 caagattctc agaagagccc gaacctgacg aatcgccggc ctgcacgcta cggccatagc
     3421 cagagataat accgctagtc acggtatttt gaaactgtcc gagcgcattg ccgatagcca
     3481 ctacatcatc accgacctgc acggttgatg aatcaccgag gacagccggc gtaagcttat
     3541 gccccttgat gtcgttgatt ttcaggaagg cgatgtccag attgtcatta tcgctcgtcc
     3601 gaccaacgac cgaaacattt tttagttcag tcccatcact caacgtaaca ctgacattag
     3661 tcgtaccagc cggtaccaca tggcgattag taataattaa gccgtcggaa gctataacga
     3721 taccggtacc ggccgcctgc tcctgctccg gctgcgagaa gccaaacaag ccgaagccgt
     3781 cttgagagct gctagaagag gtgatgttga cgttgacgct gaccatgctc ggccccaccg
     3841 tcttagcaat ctgactaagc agctggctgg tactggtgac gattttcttc tggccgctca
     3901 gcgagccact aataacaccg gtattgttag cgtggttttc tacccaagcc cccgcgaagc
     3961 cgccaagcat tgcaaccaag acaatgccaa taacgagccc ggcccctcct tggcgtagac
     4021 gtccgaggtc tggctttgct atacgaggca tctccggtac ggcaaagctg accgtgcgtg
     4081 gcggccggcg cgaaccgctg gagacactgt aaacttccgg attcttcgac ttgtcttcgc
     4141 tactcacacc acaccccctt atacaacctt attaccccag tcggaatagg ccaaaaccac
     4201 caacacgatc gccagcataa taaagataaa ctgccggcgc atgcccctgc tcagcttgtc
     4261 attgtgatcg agataataca gtaccgccaa gccgtaaccg agggcactca ggagcagtgt
     4321 cggttgggcc aagccgtgat agtacagcag ccagtggctg agcagccagg ccagcgccgc
     4381 gccgaaatag ccccatatat aagacaacat gcgggcatac gtttcctcga aagcatcgaa
     4441 gtaatgccgg gccgcgagaa aacagatcaa gccggttatg agcgtcaaac catacaccgg
     4501 cccgttggtc caggtaagat acaacgccat caaagccgtt agctggccga tgaaggcttg
     4561 ggccgtaatc attggcagcc cggttgccgg cttaatagcg atcagccaaa cggcgtagag
     4621 gaccgccagc aggagctgca accacacttg accactagca tgggtcgtaa acaacacaat
     4681 tgatacgccg accatcaagt cgaccgcgtt agcgcggaca ttggcagccc agaaacgagg
     4741 tcgtacagca aacatacgcc atttacttaa cacaatgatg ctgagcgcga gctgaacgaa
     4801 atttaagcga accagtataa aaaccgcggc cggcaaaata accagcagta tcaggtgtaa
     4861 aaagtgggaa accccactag ttggcttgag gcgttgtagg agtggtcgca tcactgccta
     4921 ctataataac aaagtcggcg ccattcgttt gtatggttga atccggcagg ctagtaagtg
     4981 ctgtgctgtt aaagcgctgc tcgaggtagt gcttggtgta cttatccttg ccgttcgtca
     5041 gatcgatgag tgtcgtctgg gcccagttat tagttgtcgc attggccgtg ccgacgacgt
     5101 tatagccgta ggccttgagc gtcgtggctt ctttcgaggc tagcccaggg acattcgtgc
     5161 cgtttagcac cagaaccttg gcattttctt tcaggatata gggatccttt agttgactgc
     5221 gaacaaacga ttgaatgtcg gtgtagttaa agaggccggc ggccggcagg tcgatggact
     5281 gccctgccat agtgccggtt ttgatgtaat tgttcggcgg atcggccagg ccaatcgact
     5341 gcgtgtccac atctgatata tttttgacaa tgctataggc gcgcgtggca tccttgatcg
     5401 agaaatcagt cgccacattg ttgccaaaag agctcagtaa gccggatatt tttagcggat
     5461 tgctcagcgt gccaagcgtc tccaccttgg ttttgagggc tagaagcaca gcgcgctggc
     5521 gctgggcccg ggcaaagtca ctagtcgttt cgcgtgaacg cacatagata agggcatgtt
     5581 tgccgtcaaa agtctgcggt ccggccttgg ctagcaccga attaccgtga ttttcccaag
     5641 ccatagtcgg gtcgaccaga tcagtcggca cgttcacact caccccgccg acagtatcga
     5701 ccgcttgctg gaaggcctgg aaatccacca gcatattata gtgaatggtc actccgagaa
     5761 cactttccac cgtctgatct gccaagtcaa atccggcttg aatggcctgg ggatcggtgc
     5821 tgccgggtct aaccttgtgc aaataccgga attcaccagt ttcccaggcg gcattgagct
     5881 tcatggcgcc ggcatccggc acgtcaaccc agaggtcgcg cggaatgctg atgagcgttt
     5941 gggtatgatt caccgggtcg atactatcta gcatcatggt atcggtgaga tccgggccgt
     6001 cgtggttacc gccgccccgt cccattaata agatattgac gcggccgtcg ccctcaccct
     6061 taaggagctc aggattgacg ttagcttgca gcgccgcggc cgttttggaa ccacctttaa
     6121 acgctttatg cagcttgagg taaccctggg tgaaaaagag gccaccgccc accatcatta
     6181 agatcaaggc aaacgccatg cccctgaaga gccagcggcg ggctgtcgcc cagcgaccgt
     6241 gagccagcag tgaatcaatt cgccgcaaag tgctttagcc accgggcaac gacatatcta
     6301 gcggtgtgtg gcggcgctca ccgagcagcg gtgcctgtac cgccggcgtc ggcctaggtc
     6361 taggaggctg gggcgtcgga cgcgctaaac taaccccggg cacaggttgg tatgaactcc
     6421 ccgagcgatt ttgtgttatt tggcgttctg tttgtatttc acgccgccga aatcgtgacg
     6481 tatccatctc taactctccc agcataccgc gaaagtgctt atgtgtaaag cccccgctcc
     6541 cacctgtggt atagtggata cctgtatgga caatgacata aaatccccgc ctgaaggtcc
     6601 ggccttgcca tccaagcctt ctgctccccg cgcccctgtc actccaccgg ccccggtccc
     6661 gaaacaggga cgaattatgc ctaccaaagg tacttctgcc tggcgtgaac tgttttcgac
     6721 tgtcggtatt ttgctaaccg ctctcgctgt cgccctgctc atcatcgctt ttgtcttccg
     6781 ctcctaccag gtcgacggcc ctagcatgga aagtaccctc caaaacggcg acaaactgat
     6841 tatctggaag gtgccccgca gctgggccag cgtcacccgt cacgcttata ttccggaccg
     6901 tggtgatgtc atcgtcttca cggaaagcgg tttgagccag tttggtcaga ccgataccaa
     6961 acagctcatc aaacgtgtta tcggattgcc cggcgaccgc gtggtggtcg ccaacggtat
     7021 ctacactata tataataaga gtcacccgca gggcttcgac cctgacaaaa gcttgcccta
     7081 cggcaaaaac atccctacca cctctggtaa cgtggacgtc acccttggca aaaaccagct
     7141 gtttgtgtcc ggcgataatc gcccggattc gcttgattca cgcgcttttg gcccaatcaa
     7201 cgctagtcag gttgtcggaa agctagtgct acgggtcttt cctattaata aagccgaagt
     7261 tttttagtat agtgagggta tgaaccaaac accaaaacgg ggtttaggca gagggtttga
     7321 gtcccttttg ccggctgatt ttaacaaaac tgccctgctg acacctgagg atcggatcga
     7381 gaaaatcccg gtagccgatc tcgtgcccaa tccgcaccag cccaggcgtc atttcgacga
     7441 gaaggccctg caagaattag cggcttcgat caagcgccac ggtgttgttc agccgttagt
     7501 tgtcaccgct gttagtgcgg gcaaatatac tttgatcgcc ggtgaacgcc gctggcgggc
     7561 gtcgcgcttg gccggactgc aaaccgtgcc cgccattgtg cgcaccacca aggaaatcga
     7621 acgcttggaa atcgcgttaa tcgaaaacgt ccagcgcgtc gaccttagcc cgctggagca
     7681 agccgcctct atccaaggct tgcacgagca attctccctt tcttacgaag aaatcgctaa
     7741 gcgcctcggt aaagctccct cgaccatcag taatagtgtc cgcttgctcg acctccccga
     7801 agccgcccgc gacgccttgg ccgctcgcca aatcagcgag ggtcacgccc gcgccatcct
     7861 ggcccttcgc ggcgatcacg aacgccaaac ctacctgtta aaaaccatca tcgggcaagg
     7921 ctggagtgtc cgccaagccg agcgttttgt cttaagcgtc aagcaaggcg tcaaagaaac
     7981 cgctcgggcc cacgcccgca ctgagctcga aacgcccgcc acgaagaagc tcagcaagca
     8041 gcttggc
//
LOCUS       DATNGP010000200         2598 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_2886961, whole genome shotgun sequence.
ACCESSION   DATNGP010000200 DATNGP010000000
VERSION     DATNGP010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2598
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2886961"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1383
                     /locus_tag="VN778_05210"
     CDS             <1..1383
                     /locus_tag="VN778_05210"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012236.4"
                     /note="Produces ATP from ADP in the presence of a proton
                     gradient across the membrane. The beta chain is a
                     regulatory subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HXR50394.1"
                     /translation="PPWYQGRALEGNHHWYQKIEGRSGRLMAGVIVSVKDLVVRAQFD
                     DDSPALQELVTVENGHGTQLLVDHLEPGGIAFCLNVRTDLRIVKGMAVERTHKGIEIP
                     IGEVTIGRVLNALGDPLDGQAPIEGENLQRKDILKLPPKSTNFSTAKPEILETGIKVI
                     DFFTPFVKGRKIGIIGGAGVGKTVLTMELINNIAKSGSGLSFFSGIGERIREGHELYE
                     TLKENDLLKNTCMFFAQMNENPIQRSLIGISATASAEYFRDEQHKDILFFADNMFRHI
                     QAKNELATILDQIPNEGGYEPTIFSDVKVLQDRLSSNENGSITSVQTIYVPADDISDP
                     AVQFIQHEMDSIIVLSRKVAEQGIRPAVDLNITTSSLLTPEIVGERHYLLSVQVQALL
                     QKYVSLKGIIAIIGENELSPADRSDYAKAKKLIANFTQNMSVMTKHTGVEGHFFTREE
                     TLKSIEEIVV"
     gene            1380..1757
                     /locus_tag="VN778_05215"
     CDS             1380..1757
                     /locus_tag="VN778_05215"
                     /inference="COORDINATES: protein motif:HMM:NF014838.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50395.1"
                     /translation="MSDAAAQGVNDQKAIDAQDTHETKADKEARQAVEEYSRVEANGQ
                     PSMRVKIYSPFRDYFDDQAFSISAENATGPFDILPKHHNFISLLLPCEVVLRSVKQGD
                     QRIRISGGLMHVKADRVIVFLDV"
     gene            complement(1735..>2598)
                     /locus_tag="VN778_05220"
     CDS             complement(1735..>2598)
                     /locus_tag="VN778_05220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50396.1"
                     /translation="PHGLMARLKRFNLYILLFIFLLVVAGGIVTIAYFQSKKAATTST
                     LKTQQLTQQTLQQVANSDATIGNSQQVLNVESSAVFAGKVLVREDLEVAGSLQIGGTV
                     ALNDLTVSGTSQLGQVQVNKNLSVAGDTGIQGAATIAKSLQVSGTGSFSGALTAPQIT
                     TSNLQLNSDLVLTHHIIAGGGTPGRSTGPALGSGGTASVSGSDTSGSVSINTGSSPAV
                     GCFITVNFTSKYNTTPHILITPIGSGAAAWPIMSTAQLPVSAFATPRRHHRAPPSALI
                     TLSSTKRRGRR"
BASE COUNT          629 a          659 c          735 g          575 t
ORIGIN      
        1 ccgccgtggt atcaaggacg agcgcttgaa ggaaatcatc attggtatca aaaaatcgaa
       61 ggtcggagcg gcaggctgat ggctggggtt attgtttcgg tcaaagactt ggtcgtgcgg
      121 gctcagttcg acgacgattc acctgccttg caagagctgg tgacggtgga aaatggccat
      181 ggtacgcagc tgcttgtgga ccatttggag ccgggtggca tcgctttttg cctcaatgtc
      241 cggactgatt tgcgcatcgt caaaggcatg gcggtagaac gaacgcacaa aggtattgag
      301 atcccgattg gcgaggtgac gattggccgt gtcctgaatg ctctgggcga tccgctggac
      361 gggcaagcgc caattgaagg tgaaaacttg cagcgcaaag acatcttaaa attgccgccg
      421 aaatccacga acttctctac cgccaaaccg gaaatattgg aaacgggtat caaagtcatc
      481 gacttcttca cgccgtttgt gaaaggccgc aaaatcggca ttatcggtgg tgccggtgtc
      541 ggtaagacgg tgctgaccat ggaactgatt aataacatcg ccaaaagcgg cagcggcttg
      601 tcattcttct ccggtattgg tgagcgtatt cgcgagggtc acgaactcta cgaaacgctc
      661 aaagaaaacg atctgctgaa aaacacctgc atgttcttcg cacagatgaa tgaaaatccg
      721 attcagcgct ctttgatcgg catttccgct acggccagcg cggagtattt ccgcgacgag
      781 cagcacaagg acatcctgtt ttttgcggac aatatgtttc gtcatataca ggccaagaac
      841 gaactggcaa ccattctcga tcagatcccg aacgaaggcg gctatgagcc gaccattttc
      901 tcggacgtta aagtgctgca ggaccgtcta agctccaatg aaaacggctc tattacttcg
      961 gtgcagacca tttatgtgcc ggctgacgac atcagtgacc cggcggtgca gtttattcag
     1021 cacgagatgg attcgatcat tgtgctctcc cgtaaagtgg ccgagcaggg catccgtccg
     1081 gctgtcgatt tgaacataac gacatcttcg ctactaacgc cggaaatcgt cggcgaaagg
     1141 cactatttgc tcagcgtgca ggtgcaggct ttgctgcaga aatatgtgtc acttaaggga
     1201 atcatcgcca ttattggcga aaacgaatta agcccggccg accgctcgga ttacgctaaa
     1261 gccaagaaac tgattgccaa cttcacccag aacatgagcg tcatgaccaa gcacaccggt
     1321 gtcgaaggcc acttcttcac gcgcgaggaa acgctgaaaa gcatcgagga aatcgtcgta
     1381 tgagtgatgc cgcggctcaa ggggtcaacg atcagaaggc gattgacgct caagataccc
     1441 atgagacaaa agccgacaaa gaagcccggc aagccgtcga agagtacagc cgggtcgaag
     1501 ctaacggcca acccagcatg cgcgttaaga tttactcgcc atttcgagat tattttgatg
     1561 atcaggcctt ctcgattagc gctgaaaatg ccaccgggcc gtttgacatc ttaccgaaac
     1621 accataactt catctcgctt ttactgcctt gcgaggtagt cttgcgctcg gtgaaacaag
     1681 gtgatcagcg catccgcatc tctggcggcc tgatgcatgt caaggctgac cgtgttatcg
     1741 tcttcctcga cgtttagtcg atgacaaagt aatcaaagcc gaaggaggtg cccgatggtg
     1801 gcgccgtggc gtcgcaaatg ctgaaactgg tagttgagcg gttgacataa taggccaagc
     1861 cgcagcgcct gaaccgatag gcgtgatgag gatatgcggc gtcgtgttgt atttgctggt
     1921 gaaattgaca gtgatgaagc agccgacggc cggcgagcta ccggtgttaa tactgactga
     1981 gccgctggtg tcggagccac tgacgctggc ggtgccgcca ctaccgaggg ccggcccggt
     2041 agagcgcccg ggcgtcccac ctccggcaat gatgtgatgg gtcagtacca ggtcgctatt
     2101 gagctggaga ttggaagtgg taatctgagg cgcggtcagc gcaccgctga agctgccggt
     2161 gccactgact tgcaggcttt tggcgatggt cgctgcgccc tggatgccgg tgtcgccggc
     2221 gacagacagg tttttgttga cttgcacttg tcccagctgg ctggtgccgg aaacggttaa
     2281 atcattcagc gccacagtgc cgccaatctg caggctgccg gccacttcca ggtcctcgcg
     2341 caccagcacc ttgccggcaa acacggcgct tgactcgaca ttcaagactt gctgtgaatt
     2401 accgatggtg gcatcgctgt tagcaacctg ctgcagggtt tgttgcgtta gctgctgagt
     2461 cttgagcgta ctagtcgtag cggccttttt actttgaaaa taagcgatag taacgatacc
     2521 gccggctaca acgagcagga atataaacaa taagatgtaa aggttgaaac gcttcagacg
     2581 agccatgagc ccgtgcgg
//
LOCUS       DATNGP010000201         3045 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_4269035, whole genome shotgun sequence.
ACCESSION   DATNGP010000201 DATNGP010000000
VERSION     DATNGP010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3045
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4269035"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /locus_tag="VN778_05225"
     CDS             complement(<1..239)
                     /locus_tag="VN778_05225"
                     /inference="COORDINATES: protein motif:HMM:NF025118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HXR50397.1"
                     /translation="MQENLFADAVLSVSDFVALTNQTLEFALPQVTIRGELANFRVSK
                     NKWLYFDLKDDAATVRFFGTVYQLTTPLEDGMVLRV"
     gene            381..1607
                     /locus_tag="VN778_05230"
     CDS             381..1607
                     /locus_tag="VN778_05230"
                     /inference="COORDINATES: protein motif:HMM:NF013347.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50398.1"
                     /translation="MSASLVILGRLPGLGTAELESVFGADKLHAASSQAVSLDVDPGE
                     IDFKRFGGAVKLCKLLTTLKTTNWKAIEKYLIQTSPDQAKNLPEGKMHLGLSMYGLSV
                     GPKQLLATGLSIKKAVQRETSKSVRLVPNKTAELNAAQILHHRLTGGNGWELVLVRSG
                     DKTLLGQTVAAQDIDAYAARDFDRPKRDAKVGMLPPKLAQIIVNLSVSNADPAHGGVL
                     LDPFCGTGVILQEATLMGFDVYGSDLDPRMVDYTDKNLLWLMGQPFSTIHRPDEYADD
                     ATWRYFRLEQGDATTHQWSPAPSFVASETYLGRPFTSMPSAEILAQTIAECNTILRKF
                     LQNIQPQLQPGARLCLAIPAWQVRPNEFKHLPLIDLLSDLGYNQVRFTHVRDTGLLYY
                     RPDQIVARELLVITRK"
     gene            1609..1881
                     /locus_tag="VN778_05235"
     CDS             1609..1881
                     /locus_tag="VN778_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HXR50399.1"
                     /translation="MSHVKAGGTAKNVKDSPGQRLGVKRYAGEKVTVGQVLVRQVGST
                     KRAGQGTKLSANYTIHAHKDGVVAFKTRRVRRFTGKTVRRTEVVVE"
     gene            complement(1838..2278)
                     /locus_tag="VN778_05240"
     CDS             complement(1838..2278)
                     /locus_tag="VN778_05240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50400.1"
                     /translation="MINRFSSSPIFGEFHVVSKVVPPSEFGVIAVPRAKVAEVARLVH
                     EAGHDIKVIALELLMPEDVAARYERFEDGGTNAAEARVPGRLAARLGRLMTKFLRVGT
                     KNVKTIRQLRYAVRRRGLAFGWTLAKYPELLIRQQLRCGGRFCR"
     gene            complement(2378..2581)
                     /locus_tag="VN778_05245"
     CDS             complement(2378..2581)
                     /locus_tag="VN778_05245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50401.1"
                     /translation="MYASAGNPITPIRLRQEISELEQSVAAADARGPTEEFWPALINE
                     ELKRVLADTRAQLKMVAAEPEAA"
     gene            complement(2597..>3045)
                     /locus_tag="VN778_05250"
     CDS             complement(2597..>3045)
                     /locus_tag="VN778_05250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50402.1"
                     /translation="VTTRLEASALPFDRGTVSLMVDAVLNRVQRIARISEVAADEVRE
                     LLVSNQLAGADITPVLGKSPVDPIYKQLRLLDIYEQDPDAARAIASNHVAGFHASRSG
                     GLLSTLRHGLLPAAEAPPTRGDVPIFHGERVFSQGKQADISYADWT"
BASE COUNT          746 a          890 c          737 g          672 t
ORIGIN      
        1 acccgcagca ccataccatc ttccaggggc gtcgtcagct gatagaccgt gccaaaaaac
       61 cgtacggtcg cggcgtcatc tttcagatca aagtacagcc atttattctt gctaacccga
      121 aaattggcca gttcgccccg aatggtgacc tgcggcaagg caaactcgag cgtctgattg
      181 gtcagcgcta caaaatcact gacgctgagc accgcatcgg caaacaagtt ctcttgcata
      241 ccaccattgt acgctgtagt tcgtcatcct cgggcgcgac ccggggatcc agtaagcgtt
      301 cttaggtttc agacaagact atagcagtct agattcccac cttcgtggga atgacaaaat
      361 acaggtatac ttaaaatact atgagtgcaa gcttggtgat tttgggcaga ctgccgggtt
      421 taggcactgc agagttagaa agcgtttttg gcgccgacaa attacatgct gcctcgagcc
      481 aggccgtctc gcttgatgtt gatcccggcg aaatagattt caaacgattc ggcggcgctg
      541 ttaagctctg caagctactt acgacactga agaccaccaa ctggaaagct atcgagaaat
      601 atctgatcca gaccagcccc gaccaggcca agaatctacc cgaaggcaaa atgcacttgg
      661 gtctaagcat gtacggcttg agtgtcgggc caaagcagct gctcgccact ggcctgagca
      721 tcaaaaaagc agttcagcgt gagacatcaa aatcggtgcg cctggtgccc aacaaaactg
      781 ccgagctcaa cgccgctcaa atactgcatc accgcttgac gggcggaaat ggttgggaac
      841 tcgtactagt gcgcagcggt gacaaaaccc tgctcggtca aacggtcgcc gctcaagaca
      901 ttgacgctta tgcagcccgc gattttgacc gccccaaacg cgacgccaaa gtcggtatgc
      961 tgccgcccaa gctagcgcag attatcgtca acctgtcggt cagtaacgcc gatccagctc
     1021 atggcggcgt gctacttgac cctttctgcg gcaccggcgt tatcctacaa gaagctacgc
     1081 tcatggggtt tgatgtctac ggttcggatc tagaccccag gatggtcgat tacaccgaca
     1141 aaaacctgct atggctcatg ggccagccgt tctcaaccat ccatcggccc gatgagtacg
     1201 ccgacgatgc cacatggcgc tacttccgcc tagaacaagg ggatgcaacc acccaccagt
     1261 ggtccccggc tcccagtttt gtagcgagcg aaacgtacct cgggcgaccg ttcaccagca
     1321 tgccttccgc tgagattctg gcgcaaacaa ttgccgaatg taacaccatc ctcagaaagt
     1381 tcctgcagaa tattcagccc cagctgcagc caggcgctcg cctctgcctc gccattcccg
     1441 cctggcaagt tcgaccaaat gagttcaaac atttgcctct gattgacctc ttgagcgatc
     1501 taggctataa tcaagtacga tttacgcacg ttcgggacac tggcctgctc tactatcgac
     1561 cagatcagat tgttgcccgc gagctactag taataacgag gaaataagat gtcacacgta
     1621 aaagccggcg gtaccgccaa aaacgtcaaa gattcacccg gtcaacgcct cggtgttaag
     1681 cgctacgcgg gtgaaaaagt gactgttggc caagtgctgg tccgccaggt cggctcaacc
     1741 aagcgtgctg gtcagggcac caagctcagt gctaactaca ccattcatgc ccacaaagac
     1801 ggcgttgtcg ccttcaaaac ccgccgtgtc cgccgcttca ccggcaaaac cgtccgccgc
     1861 accgaagttg ttgtcgaata agtagctcgg gatacttagc tagcgtccaa ccaaaagcca
     1921 gccctcggcg tcttacggcg taccgaagtt gtcgaatagt ttttacgttt ttcgttccga
     1981 cacgcaaaaa tttagtcatc agcctaccaa gcctcgccgc caacctgcca ggtacacgcg
     2041 cttccgcagc attcgtcccc ccatcttcga accgttcata tctggcagca acatcttcag
     2101 gcataagtag ctctagcgca attactttga tatcatgacc agcttcgtga accaggcgtg
     2161 ctacttctgc aactttagct cgcggcacag ctatgactcc aaactcgctc ggcggcacta
     2221 cttttgacac aacatgaaat tccccgaaga tgggactaga agaaaatcta ttaatcatgg
     2281 gctggctata ggctgtgtct ccgtacgatg agccaatcgg atagttgctt gcatttatac
     2341 cgtacgcaac gggaaagttc tcttccgtaa gccgcactca agcagcttca ggttcagccg
     2401 ctaccatctt gagctgagca cgagtatcag ccagtactcg cttgagctcc tcgttgatga
     2461 gagctggcca aaattcttct gtaggtcctc gcgcgtcagc cgccgcaacg gattgctcta
     2521 attccgagat ctcctgccta agacgtattg gtgtgatcgg gttgcccgct gaagcgtaca
     2581 tacctatcga actaggttac gtccaatcgg cataacttat atccgcctgt ttcccttgag
     2641 aaaatacacg ctctccatgg aatataggta catcacctcg cgtcggcggc gcttcggctg
     2701 caggcagcaa gccgtggcgt aatgtgctca aaaggcctcc agaacgactg gcgtggaatc
     2761 ccgcaacatg attactcgca atcgccctag cagcatcggg atcctgttcg tagatgtcaa
     2821 gcaatcggag ctgtttatag atagggtcga ccggtgattt tcccagaaca ggcgtaatat
     2881 cggcacctgc cagctggtta ctcaccaaca gctcacgtac ttcatctgca gcaacttcac
     2941 tgattcgtgc gatacgttgc acccggttca ggacagcgtc taccatcaga ctgaccgtcc
     3001 ctcggtcaaa tgggagagcg gacgcctcaa gcctagtcgt gaccg
//
LOCUS       DATNGP010000202         4904 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_2911533, whole genome shotgun sequence.
ACCESSION   DATNGP010000202 DATNGP010000000
VERSION     DATNGP010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4904)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4904)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4904
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2911533"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1222)
                     /locus_tag="VN778_05255"
     CDS             complement(<1..1222)
                     /locus_tag="VN778_05255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50403.1"
                     /translation="MANRLPTPGGDDGSWGDILNAYLEVSHNSDGTLANNTVGTNQLQ
                     SGSVTNTQLDSATQSAIAKASNSVQQVNNKSPDIGGNVSLAASDVSAIPVSQLGAVSG
                     VATLDGSGNVPASQLNNVPSSSVNITSPDGSITVGGTASAPTLESVVPSVFSRTGAVV
                     ARSGDYNATQIPNVVTLVTQSATPAIDTDNANFFSITGLAQAITSMTTNLTGTPVHGQ
                     FIWIEITDNGTPQAITWGSSFASASVTLPTTTVASTTVRVGLTWNTITSTWDAVAGGS
                     ASGGGGGSFTPIRTAYTTAGSNSFTIPAGCMTMTVTCIGGGGGGGSGRQGAASTACVG
                     GAGGGGGGLSIQRFLASDFSSPVTVVVGVGGSGASAQTTADTNGTAGGSGGQSKFGTA
                     LVAGGGVGGAAGTAS"
     gene            1491..2186
                     /locus_tag="VN778_05260"
     CDS             1491..2186
                     /locus_tag="VN778_05260"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HXR50404.1"
                     /translation="MSSPLFRVQLFPAEGSDKLACVVAGYRGKIKGYRRVIETLNNYG
                     YNVVAYEHSPAVLTSGDPHKLIDLVDGINNDFSERIISYKTIICVGASIGAGLCFALQ
                     AQFPTITFGIYAAAGVSPPENVFEAPLFYFVRKKFAKAGIDPQKLKKLWQDVDIVPEK
                     PLNLTNPFLMVLGKRDKIVNYQKALVTLHKWQAAGKPITIMTKDNVGHAGTTRWYKKH
                     FEELLVEAEKLPL"
     gene            complement(2226..2696)
                     /locus_tag="VN778_05265"
     CDS             complement(2226..2696)
                     /locus_tag="VN778_05265"
                     /inference="COORDINATES: protein motif:HMM:NF013231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HXR50405.1"
                     /translation="MNTKQDLEKQVAIAAREYGISSVLFRNAIGDKLGLNVTDMECLG
                     LLFFKITCTPTGLARYTGLTTGSTTAMLDRLEKAGLIKRKPNPNDRRGVLIDVEKKSV
                     KTVGPMFAEARKAQDQLVASYSEKELQTIADFLKRFTAVFDEEREKVINKSKSA"
     gene            2781..3113
                     /locus_tag="VN778_05270"
     CDS             2781..3113
                     /locus_tag="VN778_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010982768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXR50406.1"
                     /translation="MAQNIKVIVYPVQDLEKAKAFYGKFLGVEPYADGAYYVGYKVGD
                     VEVGLDPNSKVGPIGYTDTEDIKSSIQGLVDLGGEVVKDPTDVGGGLLVAQVKDADGN
                     VVGFRQQP"
     gene            complement(3120..>4904)
                     /locus_tag="VN778_05275"
     CDS             complement(3120..>4904)
                     /locus_tag="VN778_05275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50407.1"
                     /translation="DGPGTSGSQSISSVTGGSLTWTLCKRTNAQPGTAEIWTAWAANP
                     LSSAVIKATRAKGSYRGDITIAAFEGASSTIGAVNGSSATSGAPNNTLTTTTAGAWVW
                     GVGNDYDNAVSRTIGSNQTLVDQYLSPSGDTYWTQRQNAVTPSAGTAVTINDTAPTGD
                     RWDLSTIEILPGVVDVTPPTVPTNLSATANSTSQVGLSWAASSDDTGVAGYNVYRNSV
                     QIGTTTATSYTDNTVAANTSYTYTVQALDASGNASANSDPASVTTPADTTPPVISNVA
                     SSAVTTTSANISWTTDEASTSQIAYGETAAYGSTTTLDSTLATSHSQALSGLSASTTY
                     HYQVVSTDASGNTATSADNTFTTPAVNPTAPTIDPSTPAATKVANNVSTTTSPSFSPP
                     ANTVIYAVFSMDSASYNGTITTVQSVTNSGTPLTWHLLGRNNNYNSTTGGFLEVWWAY
                     NPTAQTNIASTATFSQATKNVTPPVGDFQILVMDNAAPDQSAAAWNANWLISSYGNAP
                     TVNVTTTKANSQVFGVFDNWNNSETPIPGTNQSIQSIVLNTADVDGYWIQKQNSPTAT
                     AGTSVTMNATDPGSANQWRAIAWEVLAN"
BASE COUNT         1086 a         1190 c         1390 g         1238 t
ORIGIN      
        1 tgctcgctgt tcctgccgca cctccaacgc ccccaccggc aaccagcgca gttccaaact
       61 tggactgtcc tcctgacccg ccagcagtgc cgttagtgtc cgcggtcgtt tgggccgacg
      121 ctccacttcc gcctacccca acgaccacag ttactgggct tgagaagtcg ctggctaaga
      181 accgctggat gctgagcccg ccgccaccac caccagcgcc tccgacgcag gcggtcgatg
      241 ccgcgccctg gcgaccactt ccgccaccac cgcccccgcc aatgcaggta acggtcatcg
      301 tcatgcagcc cgctgggata gtgaaactgt tgcttccggc agtcgtgtag gcggttcgga
      361 taggcgtaaa gctaccgcca ccaccaccgc tggcactacc tccggcaact gcgtcccacg
      421 tgctagtaat cgtattccat gtcaaaccta ctcgcacagt agtgctggct acggtagtcg
      481 taggcagcgt gactgatgcg gaggcgaagg acgagcccca tgtgatagct tgcggcgtcc
      541 cgttgtcggt gatctcaatc cagatgaact ggccgtgaac gggcgtcccg gttaggttcg
      601 tcgtcatcga cgtgatcgcc tgcgcgaggc cggtgatcga aaagaaattg gcgttgtcgg
      661 tgtcgatcgc gggagtggcg ctctgcgtga caagtgtgac gacgttaggg atctgggtgg
      721 cgttgtagtc tcctgaccgg gcaacaaccg cgcctgtcct agaaaatacc gagggaacca
      781 cagactctag agtcggggcg cttgctgttc ctccgacggt aatggacccg tcaggactag
      841 taatattaac cgagctacta ggcacattgt ttagctggct agccggcaca ttgcctgatc
      901 catctagcgt ggctacccca ctaactgctc caagttggga tacggggatg gcgctaacgt
      961 cagaagcggc aaggctaaca ttcccgccga tatccgggga tttattgttt acttgctgca
     1021 cagaattgct cgctttggct atggcggatt gagtagcgct atctagttga gtgttagtga
     1081 ctgagccgct ttgcagttgg ttagtcccaa ctgtattgtt agccaaggta ccatcactat
     1141 tgtgtgagac ttccaaatag gcattgagga tatctcccca actaccatca tcgcctccag
     1201 gcgtgggaag cctattggcc atactaattt agcctcctgg tgctggctcc ggcgtagtca
     1261 ctaccatcgg agcttatgga taagaattca gcccagctca tctgtttttg caatagttgg
     1321 ttaagtagac gattgcccat gatttttgtc tctaaatttt gcctattaag aatcagctat
     1381 attgtagcat aagctttatt ttttacattg aggtgcctag aagtctttca gcttttgtaa
     1441 gccaggatta aatcagggta gaagggagct ttgcggtaaa attatgttac atgagcagcc
     1501 ccttgtttcg cgtccagttg tttcctgctg aaggtagcga taagcttgcg tgtgtggtgg
     1561 caggctaccg aggaaaaatt aaaggctata gacgcgtcat cgaaacactc aataattatg
     1621 gctataacgt cgtcgcttat gagcatagcc cggcggtttt aacgagtggt gacccgcaca
     1681 aattaatcga cctggtggat ggcattaaca atgatttttc ggagcggatc atcagctaca
     1741 aaactattat ctgcgtggga gccagcattg gcgccgggct gtgcttcgcc ttgcaggcgc
     1801 aatttcctac tattacattt ggtatttatg cggcggcagg cgtatcgccg ccggaaaatg
     1861 ttttcgaagc gcctttgttt tattttgtgc gaaagaaatt tgctaaggcc ggcatcgacc
     1921 ctcagaagtt aaaaaagttg tggcaagacg tcgacatcgt gcctgagaag ccgttaaact
     1981 tgaccaaccc attccttatg gtgctcggga aaagggacaa aatcgtcaat taccaaaagg
     2041 cattagttac gctacataaa tggcaagctg ccggcaagcc gataacgatc atgacgaaag
     2101 acaatgtagg ccatgccggc actacccggt ggtataaaaa acattttgaa gaactgctag
     2161 tggaggccga gaagctaccg ttgtgatttt atgccgggtt gttacgtgag acttaccaag
     2221 tatctctaag cgcttttact cttgtttatt actttctccc gctcctcatc aaaaacggcg
     2281 gtgaagcgtt ttaagaaatc ggcgatggtt tgtagctcct tctcagaata actagctacg
     2341 agctggtctt gggcttttct ggcttcggca aacatggggc cgactgtttt caccgatttt
     2401 ttctcgacat cgattaagac gccgcgccta tcgttcgggt ttggtttacg cttgataagt
     2461 ccggcctttt ctagccggtc aagcatggcg gtggtggagc cggtcgtgag gccggtgtaa
     2521 cgcgctaaac cagtcggggt gcaggtaatc ttaaaaaaca agaggcccag gcattccata
     2581 tcagttacgt taaggcctaa tttgtcacct atagcatttc gaaacaaaac tgagctgatg
     2641 ccgtattcgc gagcggctat agcaacttgt ttttctaaat cctgcttagt gttcatacaa
     2701 aaatcccgtt gacaataact ctaatatcaa gtaacttgat tatacagcaa tacatatata
     2761 atttcaatag gaggctcgta atggcacaaa acattaaagt gatcgtgtat ccggtccagg
     2821 atttagaaaa agctaaggcg ttttatggaa aatttcttgg cgtagagccg tatgcagacg
     2881 gcgcttatta tgtcggctac aaagttggcg acgtggaagt aggcctggac ccgaattcga
     2941 aagtcgggcc gatcggctat acggacactg aggatattaa aagcagcata caaggtttag
     3001 tggatctcgg tggcgaggtc gtaaaagacc ctaccgatgt aggcggcggt ctgttagtgg
     3061 cccaagtcaa agacgccgat ggcaatgtcg ttggcttcag gcagcaaccc tagcactatt
     3121 tagttggcca ggacttccca ggcgatggcc cgccactggt tggctgaacc tgggtcggta
     3181 gcgttcatgg taacgctcgt tccggcggta gcggtgggtg aattctgctt ctggatccag
     3241 tagccgtcga catcggcggt gttcagcacg atggactgga tggactgatt ggtcccgggt
     3301 attggggttt cagagttatt ccagttatcg aaaacaccaa agacttgaga gtttgctttg
     3361 gtagtggtga cgttgacagt aggcgcattg ccgtatgaac ttattagcca gttagcattc
     3421 cacgcggccg ccgattggtc aggcgcggca ttgtccatga ctagtatttg gaagtcgccg
     3481 acgggtgggg tgacattctt ggtagcctga ctgaaggtgg cagtggaggc aatgttagtt
     3541 tgagcggtcg gattgtaagc ccaccaaact tctaggaagc ccccggtcgt agagttgtag
     3601 ttattgtttc gcccgagcaa gtgccaggtc aaaggggtgc cgctgttggt aaccgactga
     3661 acagtggtaa tagtgccatt gtagctggcc gaatccatcg agaagacagc gtagattacc
     3721 gtattcgccg ggggtgaaaa gctcggtgag gtggtcgtcg acacgttgtt ggcgactttt
     3781 gtagcggccg gcgttgacgg atcaattgtc ggcgcagtgg ggttgaccgc cggtgtagtg
     3841 aaagtattgt cagccgaggt tgctgtattg ccgctagcat cagtcgaaac gacctggtag
     3901 tgataggtcg tacttgcgct caagccgctt aaagcttgag agtgactggt ggctaacgtc
     3961 gagtcaagcg tggtggtcga gccgtaagca gcggtttcgc cgtaagctat ctggctggtt
     4021 gaggcttcgt ccgtcgtcca gcttatattg gcggaggtag tggtaactgc gctcgacgca
     4081 acatttgaaa tgacgggtgg agtagtgtcg gcgggggtag tgacgctagc gggatcggaa
     4141 ttagcgctgg cattgccact tgcgtcaagg gcctggacgg tgtaggtgta actggtattg
     4201 gcggccactg tattgtcggt gtatgaggtg gcggtggtag tgccgatttg tacgctatta
     4261 cggtagacat tgtagccagc aactcccgta tcatcagatg aggccgccca gctcaagcct
     4321 acttgtgaag ttgagttagc tgtagccgat agattggtcg ggacagtcgg tggtgtcaca
     4381 tcaaccacgc ccggcaagat ttcgattgtt gacaagtccc acctatcacc tgtcggggcc
     4441 gtgtcgttga tagttacggc agtaccggca ctcggtgtga cggcgttttg ccgctgggtc
     4501 cagtacgtgt cacccgaggg gcttaggtac tggtcgacca acgtttgatt tgatccaata
     4561 gttcggctga ctgcgttatc gtagtcgttg ccgactcccc atacccaggc gccggcagta
     4621 gtggtggtca aggtattgtt tggtgcaccg ctggtagcac tgcttccatt cacggcacca
     4681 atggtgctac tggcaccttc aaaggccgca attgttatgt cgccgcgata tgaacccttg
     4741 gcgcgagtcg ccttaatgac agcgctgctc agcggattgg cggcccaagc cgtccagatc
     4801 tcggctgtgc ccggctgggc attggttcgc ttgcacagtg tccaggtcaa actgccgccg
     4861 gtaacgctcg aaatactttg cgatccggag gtgcccggtc catc
//
LOCUS       DATNGP010000203         2716 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_3715377, whole genome shotgun sequence.
ACCESSION   DATNGP010000203 DATNGP010000000
VERSION     DATNGP010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2716
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3715377"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..943
                     /locus_tag="VN778_05280"
     CDS             <1..943
                     /locus_tag="VN778_05280"
                     /inference="COORDINATES: protein motif:HMM:NF013377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="trehalase family glycosidase"
                     /protein_id="HXR50408.1"
                     /translation="LCHLFDKMDIVPARNSWTSVGRTQPPSLTNMAFNVYDHGGADIA
                     WLDTAMEYGLREYQRVWTSGRRFDSATGLSRYNPKYFGNRLATFESGWDISSRFADLG
                     NAVLPVDLHCRLGGYEQDFLRYATMRGDTQAQTYWQEAIEMRTDLLRKNFWDKETGFY
                     YDRDLKTGQLLPLKTLAGYYPLLFKLASSEQAEHCRQQLPVFEHPGGLANSEKLPYAK
                     RQWDYPNGWPGTNYDVITALRRNGFNEDATRLTGKWLDCVSGVFEETGELWEKYDVVA
                     KRPGLDGRYPTQKRFGSGWTGGTFVRLLNDAKEAEMR"
     gene            1010..1606
                     /locus_tag="VN778_05285"
     CDS             1010..1606
                     /locus_tag="VN778_05285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50409.1"
                     /translation="MSERQNIVIVPGLHPLTPESVQYGMYSAATAAWPKDQFAVHILQ
                     VGWHGRGETAETQQDRLREGLDTIEGPIWAIGVSAGGLAIIGAMQADEDKIPKVVTIA
                     SPLEVPESELNQLRRKPRLPRSLEGHYQTADTYLRRLGGSASSRVVSLHGKRDSRVPL
                     SWSKRNPIESYELPGRRHSHTILGGLALHREKILELFR"
     gene            complement(1733..2548)
                     /locus_tag="VN778_05290"
     CDS             complement(1733..2548)
                     /locus_tag="VN778_05290"
                     /inference="COORDINATES: protein motif:HMM:NF016972.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HXR50410.1"
                     /translation="MSISYESLVLACDIDDTLVPIHDRPGEEREAGLVALSALVSSVE
                     TMRSDFGSVYFGSVTGRSLASHQEYEAQVPVFGSAARAMDFKVTSVGSESYIRTPDGF
                     RRVASWPAAASWESYQVRRVLSDVPGLIVQPDIAQGDYKVSFTVDEQAGQEHAATVAR
                     VGACLAGSGLAANVILSSVYLDILPLGVDKGTGLYHTLGEMALQRPFIIAAGDSMNDV
                     QLLEAADLAILPRNAHPRLRDWAERTIHPQKLFLATLPFAAGIHEGLERLLNT"
BASE COUNT          739 a          704 c          700 g          573 t
ORIGIN      
        1 tctttgccat ttgttcgaca aaatggacat tgttcccgcg cgtaatagct ggacaagcgt
       61 gggccgaaca cagccaccat cacttacgaa tatggccttc aacgtctacg accatggagg
      121 cgcagacatc gcctggctcg ataccgctat ggagtacggc ttgcgtgaat atcaacgcgt
      181 ctggacaagc ggcagacgat ttgacagcgc caccggactc agtcgctata atccaaaata
      241 ttttggcaac cggttagcta cgtttgaatc gggctgggat atatcaagcc gatttgcaga
      301 cctaggaaat gctgttctgc cagtagacct acattgtcga ttaggaggat acgagcagga
      361 tttcctccgc tatgcgacta tgcgcggaga cacacaagca caaacatatt ggcaagaagc
      421 aattgaaatg cgaacagacc ttctaagaaa aaacttctgg gacaaagaga ccggctttta
      481 ctatgaccgc gacttgaaaa cgggccagct tttgcccctt aaaacgctag ccggctacta
      541 tccactgctt tttaagcttg ctagctcaga gcaagcagaa cattgccgcc agcaattacc
      601 ggtgttcgaa catcctggtg gcctggcaaa cagcgaaaag ttgccgtatg cgaagcggca
      661 gtgggactat ccaaacggct ggccagggac aaattatgat gtcataaccg cactcaggcg
      721 taatggattt aatgaagatg cgactcggct taccggcaaa tggcttgact gcgtctcagg
      781 agtgtttgaa gagacgggag agctttggga aaagtacgac gtcgtagcaa aaagaccggg
      841 attggatgga cgatacccga cgcaaaagcg ctttggaagc ggttggacag gcggaacatt
      901 cgtgcgacta ctcaatgatg caaaggaagc cgagatgcgc taagaagccg cagcagactg
      961 agtcagtaga taaccgtcat accagagtaa cgagaaaggt gagtacacca tgagtgaacg
     1021 tcaaaatata gtgattgtcc ccggcctcca tcccttaaca cctgaatccg tacagtacgg
     1081 catgtacagc gcagctacgg ccgcatggcc aaaagatcag tttgctgttc atattctgca
     1141 agtaggatgg catgggcgcg gcgaaacagc ggaaacacag caagatcgct tacgagaagg
     1201 ccttgatacg attgaagggc ccatctgggc aatcggcgtt agtgcgggtg ggctggcaat
     1261 tatcggagcg atgcaagctg acgaggataa aattccaaaa gtggtgacca ttgcttcgcc
     1321 gcttgaagta cccgagagcg aattgaatca attacgccga aaacctcgtc ttcctcggtc
     1381 acttgaagga cactaccaaa cagcagacac gtacctgcga aggctaggcg gctctgcatc
     1441 aagtagagta gtgtcgttgc acggtaaaag agactcccgg gtccctcttt cgtggagtaa
     1501 acgcaaccct atagaatctt acgagctacc gggcagaagg catagccata caattctggg
     1561 tgggcttgcg ctacacaggg agaagatatt ggaactattt aggtaatgct aggcaggtgc
     1621 gctccaaccg agttgcatcg aacgttttaa atgtttgatt ttcaagcgcc gcatttgaag
     1681 cgcacgaatt acatataact acaaacttag cggtgtattt agcaagcagt acctatgtgt
     1741 tcaataagcg ttccaggccc tcatgaatac cagccgcgaa gggcagtgtt gctaaaaaga
     1801 gcttttgcgg atggatcgtt ctttcggccc aatcgcgcag cctcgggtgg gcattacggg
     1861 gaagtatggc caagtcggct gcctctagca gctggacgtc gttcatacta tcaccagctg
     1921 caatgatgaa tggccgctgc agcgccatct cgcccagcgt atgatacagc ccagtccctt
     1981 tatcaacacc aagcggcaag atatccaaat acacgctgga aagaattaca ttcgccgcca
     2041 gccctgaacc ggcaaggcaa gcgccaactc tagcaacggt ggccgcatgc tcttgaccag
     2101 cctgctcatc aacagtaaag ctgactttgt agtctccttg tgcgatatcc ggctgcacaa
     2161 tcaggcccgg cacatcagaa agaacacggc gaacttggta ggattcccaa ctagcagcgg
     2221 cgggccaact agcgacccgt ctaaatccat cgggtgtgcg gatatacgat tccgaaccaa
     2281 ctgacgtgac cttaaaatcc atagccctag cggcagagcc aaagaccggc acctgcgctt
     2341 cgtactcctg atgcgaagcc agtgaccgac cagtgaccga accaaaatat acgctcccaa
     2401 aatcagaacg cattgtttcg accgaggaga ccagcgcgct aagcgcgacc agaccagctt
     2461 cccgttcttc gccgggtcta tcgtgtattg gcaccagcgt atcatcaata tcacacgcca
     2521 gcactaaact ttcatagctt atgctcatag ctctatgcca cgcaaatctc gaagggctgc
     2581 ggcaagccca tgctcaataa tcaccagctc agcggaattc atggtaccgg cctccacaaa
     2641 ctcgcgtacg gtttctatag gaaaagtccg ctcggctggc tggttggggt cggttggctc
     2701 taccagcggc ttagta
//
LOCUS       DATNGP010000204         2696 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_1483534, whole genome shotgun sequence.
ACCESSION   DATNGP010000204 DATNGP010000000
VERSION     DATNGP010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2696
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1483534"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1163)
                     /locus_tag="VN778_05295"
     CDS             complement(<1..1163)
                     /locus_tag="VN778_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50411.1"
                     /translation="MSAILLPSKLRQSPQYPARLKVLDFWRLVDIQGLPLLGAAASST
                     TRTLNGTAQLGTGLAGKCLNFVVDGDWLNYGENQSLQLSTMTGLVVVEMTAAASHSAL
                     FNTNVNGSFAGGFVIRTNPTGTVTALKQDTVEIGTTVKTVRKGSGVSNIAWSYNNVTG
                     KLRIAVDGVLESFTSAQTFNQGPVGRNSYYGDATQAGAHKEYLFALSASDKVSDSELA
                     EWSLNPWSIFKTPTRKIWGTFTNRVLTGENLTQNNSTSDSTIGIGGALFAFPITQSNA
                     LSSDQIVRDQYLDVSGLTQSNSASTAPIGPYGRLRSLLRNMPLHTWAQVNTTKYIDCQ
                     MPLADRPGGFTNPVDHVKVIIPWSSFAFDHVRGNLILWGGGHANYSGNQIYHW"
     gene            complement(1164..1595)
                     /locus_tag="VN778_05300"
     CDS             complement(1164..1595)
                     /locus_tag="VN778_05300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50412.1"
                     /translation="MPTITQSQGAKSGALLTMGTLAIDTYIASSAIDLGANIPLDVTF
                     EVVVTVTSPVSDQQVLLFAQLSLDNTDFGTGPTSGSSATDEADLHWIGTLPCNSTGTH
                     RKLFSLSGLPVAKYIKLVARNRTGVALTSGFVYRADITGAA"
     gene            complement(1598..1981)
                     /locus_tag="VN778_05305"
     CDS             complement(1598..1981)
                     /locus_tag="VN778_05305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50413.1"
                     /translation="MAKYVHADVLDGGLNAIKSGAIRMHLLKAYSSADSFATVTSNIV
                     ASVVMASGDYTISGADGAARILTVAARSGTASANSGATPDLHIAFTDNVSKVLLVTDE
                     TTDQVVTSGNTVNFPSLTYTSAQPS"
     gene            complement(1985..2209)
                     /locus_tag="VN778_05310"
     CDS             complement(1985..2209)
                     /locus_tag="VN778_05310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50414.1"
                     /translation="MKTIRLLKDVIGGNGEPLKEGSFYSLNEASANHWLKRGLAVEAE
                     PDKSETEELTEAKTEEVAVDAPRRRKQNKD"
     gene            complement(2206..2526)
                     /locus_tag="VN778_05315"
     CDS             complement(2206..2526)
                     /locus_tag="VN778_05315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50415.1"
                     /translation="MAVISTGTLGTSATLDAKWQQADDTSGTNAVDVGTALTQIVKAS
                     GDNKQAVMNFVPSTDYTEKRYARLVMTVGVATSDASAVVLGFDARHLPASDNDLSTVV
                     QIVG"
BASE COUNT          627 a          697 c          704 g          668 t
ORIGIN      
        1 tgccaatgat aaatctggtt gccgctatag tttgcgtgtc ctccacccca taaaatcaga
       61 ttgccgcgaa catggtcaaa ggcgaaactg ctccacggta tgatgacttt tacatgatcg
      121 accgggtttg tgaaccctcc tggacgatca gccagtggca tctggcaatc aatatatttt
      181 gtagtgttaa cctgcgccca ggtatgcaga ggcatatttc gcagcaatga gcgcaagcgc
      241 ccgtatggtc ctatcggggc cgtggacgca gagttacttt gtgtcagacc agatacgtct
      301 aaatactgat cccttacgat ctggtcggaa gagagtgcgt ttgactgagt tatcggaaat
      361 gcaaatagag cgccaccgat gccgattgtg gaatcagagg tggagttgtt ctgtgtgaga
      421 ttttctccgg tcaaaacccg gtttgtgaat gtcccccaaa ttttacgggt tggagttttg
      481 aatatgctcc acggattaag cgaccactca gctaactcgg aatcacttac tttgtcgctg
      541 gcggacagag caaacaaata ttccttgtgc gccccggcct gcgtagcgtc accgtaataa
      601 ctattccgac caacagggcc ctgattaaat gtctgggcag acgtgaaaga ctcaagaacc
      661 ccatccactg caattcttag ctttccagtt acattgttgt aactccacgc gatattacta
      721 accccagagc ctttcctgac cgttttgaca gtcgtgccga tttcaacagt gtcctgtttt
      781 aatgcggtga cagtcccggt tgggttggtt cgaataacga agccgccagc aaatgacccg
      841 ttaacattcg tattaaataa tgccgaatgg cttgctgcgg cggtcatctc tacgacaacc
      901 agccctgtca tggttgacag ttgcaggctc tgattttcac cgtaattcag ccaatcccca
      961 tcaacaacaa aattcaggca cttacccgct agtccggtgc cgagctgagc agtgccatta
     1021 agcgtgcgcg ttgtagagga agccgcggcc ccaaggagag gcagtccctg aatatcaaca
     1081 aggcgccaaa aatcaaggac tttaagccga gcaggatact gaggcgattg cctgagttta
     1141 ctcggtagga ggattgcaga cacctaagct gccccggtaa tatccgctcg atacacaaaa
     1201 ccggaagtca gcgctacacc tgtccggtta cgcgccacca gctttatata tttggcaaca
     1261 ggaagccctg acaggctgaa cagtttccga tgcgtgccgg tgctgttgca agggagcgtg
     1321 ccaatccagt gcaagtcagc ttcgtctgtg gcacttgaac cgctggtggg tccagtgccg
     1381 aagtcagtgt tgtcaagtga cagttgggcg aaaagaagca cctgctggtc gcttacagga
     1441 gaggttacag tgacaaccac ctcaaatgtc acgtcgagcg ggatatttgc accgagatca
     1501 attgcagagg atgcaatgta ggtatcaatg gcaagagtcc ccatcgtgag gagggcgccg
     1561 ctctttgctc cttgcgactg tgtgattgtc ggcattatta gctcggttgt gcgctggtgt
     1621 aagtcaggct cgggaaattg actgtattgc cgctcgttac tacctgatcg gtggtttcat
     1681 cagtcacgag taaaaccttg cttacattgt cggtaaaggc aatatggagg tcaggagtcg
     1741 cgcccgaatt ggccgaggct gttccactgc gggccgcgac cgtcagaatg cgagctgcgc
     1801 cgtcagcgcc tgaaatcgta tagtcgcctg atgccattac gacactggca actatattgc
     1861 tcgttacggt agcaaagctg tctgcggagg aataagcctt cagcagatgc atccggattg
     1921 cgccgctctt gattgcattc aagccgccgt caagaacatc ggcgtgtacg tatttagcca
     1981 ttggttaatc cttattttgt ttgcggcgac ggggagcgtc gactgcgact tcttccgtct
     2041 ttgcctcggt caattcttct gtttcagatt tgtcgggctc tgcctcaacc gcgagaccgc
     2101 gtttcagcca atgatttgca gacgcttcat tgagcgaata aaaagagccc tctttcaagg
     2161 gctctccgtt tccgccaatc acgtctttca agagacgtat ggttttcatc ctacgatctg
     2221 gaccaccgtg gacaagtcat tgtcacttgc gggcaaatga cgagcgtcga agccaaggac
     2281 tactgctgag gcatccgatg tagcgacacc gaccgtcatc acaagacggg catagcgttt
     2341 ctctgtgtag tcagtgctcg gcacaaaatt catgacagct tgcttgttat ccccgctggc
     2401 tttgacgatt tgggtcaaag ccgtaccgac atcaacagca ttcgtgccag aggtgtcatc
     2461 tgcctgctgc cacttggcgt caagggtagc cgacgtaccg agcgtgccgg tcgaaataac
     2521 ggccatcagg cgcgtataca gagccatgtc gatccagcct gaatttttcg ctccagcagc
     2581 cagggccccg ggcgcaatca cagcggctat cgctacgcgc tcgctcggtt tggtagttgt
     2641 ttgcaaatct ttttctccgt gagaattggt agaagcggag cctaagccac gctatt
//
LOCUS       DATNGP010000205         2716 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_550472, whole genome shotgun sequence.
ACCESSION   DATNGP010000205 DATNGP010000000
VERSION     DATNGP010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2716
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_550472"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..414)
                     /locus_tag="VN778_05320"
     CDS             complement(<1..414)
                     /locus_tag="VN778_05320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50416.1"
                     /translation="MPTPKNDKDPKVMDVERPGSSTPSASAKPIIVTNRPILRRDPMM
                     ATPDGLDKPVTTTLPGPVSRIAKTVRVVQPTASTPDKTEKPTTSAAKAGAVAPPKLED
                     LIAAKTADANERVSADKKEATPAPAAGVTPSDADQP"
     gene            complement(401..1198)
                     /gene="tpiA"
                     /locus_tag="VN778_05325"
     CDS             complement(401..1198)
                     /gene="tpiA"
                     /locus_tag="VN778_05325"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HXR50417.1"
                     /translation="MRKMLIIGNWKMHFTVHEASLHLHRLSERIKEHRDLEVVLAPSL
                     LTLQPLSLELDRHKFRLAAQNGFYKDEGAYTGEVSFTMLRDMVHYALIGHSERRIYFH
                     ESLDIVRDKVSAAVRNKITPVLFIGETYEEHKDGETKQVLHDQLTTALSDLTAGDVED
                     MVIVYEPVWAISTFGGILAKPDEVESAMKRIRAEVSELYGERAAQSVRILYGGSVDEQ
                     IVRGYIELPDCDGAVPGAASLNYHKFAAIIEAASRVVQERRKADADA"
     gene            1309..2373
                     /locus_tag="VN778_05330"
     CDS             1309..2373
                     /locus_tag="VN778_05330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50418.1"
                     /translation="MGENVNQRSDTPNYFPPAVRRILWLFRAGRLSNVASIDVEPEYG
                     YFARLNYRDGRHRVIRGGDLGINPGTSAELSKDKGYAKFMLKVLGLNTPQGQTFLLPD
                     WAQKVTANPKQHDNPNIRTTEAAYPYIEGNFGYPVYVKPVDGSQGTNIYRVDSRQELG
                     GVFAAYAERGVRVALIEKPIDLPDYRIVVLDGKLISAYRRDPLAVIGDGKQTVRELVH
                     AVQTQYQDGGRDTRLSPDDERLVATLGKAGLTLESVLPAGNRQTLLPISNLSAGGTSE
                     DVSDSISPRWVELAASVASNFNLRLCGLDLACSDITNSKADYSILEVNAAPGLDHYAA
                     SGEAQQQIVDDLYTRVVNNP"
     gene            2387..>2716
                     /locus_tag="VN778_05335"
     CDS             2387..>2716
                     /locus_tag="VN778_05335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="putative folate metabolism gamma-glutamate
                     ligase"
                     /protein_id="HXR50419.1"
                     /translation="MLVTAIKTPKITAGSIDLVNLLDQALPVLTDGSIVVITSKIVSL
                     CENSVIPIGSIDKEQLIAEQAELYLPPSFSRYNHHFTIIGNTLVGVAGIDESNGDGNY
                     ILWPRDAQ"
BASE COUNT          645 a          736 c          678 g          657 t
ORIGIN      
        1 tggctggtcc gcatcacttg gggtaacccc agctgcaggc gctggtgtag cttctttttt
       61 atcagccgat acccgttcat tcgcatcagc ggttttggcg gcaatcaaat cttccagctt
      121 gggcggcgct acggcgcctg cttttgcagc cgaggtagtt ggcttttcgg ttttgtccgg
      181 agtcgaggca gtcggctgga ctacgcggac agttttggcg attcgtgata ccggtccggg
      241 tagtgtcgtc gtaactggct tgtcgagtcc atcaggcgta gccatcatcg ggtcacgtct
      301 cagtatcggc cgattggtga caatgatcgg cttggccgaa gcagacggcg tactgctacc
      361 aggacgctca acatccatta ccttggggtc tttgtcgttt ttaggcgtcg gcatcagctt
      421 ttctccgttc ctgcactacg cggctggctg cctcaataat tgccgcaaat ttatgataat
      481 ttagactcgc ggcaccaggc acggcaccat cacaatcggg tagctcaatg tagccacgca
      541 caatttgctc gtcgacacta ccgccataca aaatacgtac actttgcgca gctcgttcac
      601 catatagttc actcacctcg gcacgaatgc gcttcatcgc gctttccact tcgtcaggct
      661 tggccaggat accgccaaag gtgctaatgg cccacaccgg ctcgtagacg atcaccatgt
      721 cttcgacatc gcccgccgtc agatcgctga gcgcggtcgt cagttggtcg tgtagcacct
      781 gcttggtctc gccgtctttg tgctcctcgt aggtttcgcc gataaagagc actggtgtaa
      841 tcttattgcg caccgccgcc gagaccttat cgcgcacaat atcgagcgat tcatgaaagt
      901 aaattcgccg ctcggagtgg ccgataagcg cgtagtgcac catatcgcgg agcatggtaa
      961 agctgacttc gccggtgtag gcgccttcgt ctttgtagaa gccattttga gcggccaggc
     1021 ggaatttgtg gcggtcaagc tcgaggctca aaggttgcag tgtcaacaga cttggcgcca
     1081 gcaccacctc taggtcgcgg tgctctttga tccgctcact cagacggtgc aaatgcaaac
     1141 tcgcctcgtg caccgtaaag tgcatcttcc aattaccaat aatgagcatt tttctcataa
     1201 aacttcttga tgcttatggt tttaagtgta gcacaaacgt gcctgctatc cggagtaaat
     1261 gaaacaccgc ttcacccttc cgcgatcact cgtagtatag tattggcaat gggagagaac
     1321 gtgaaccaac ggtctgacac gccaaattac tttccgcctg ccgtccggcg tatcttgtgg
     1381 ttatttaggg ctggcagatt atcgaatgtc gcctctattg atgtcgaacc ggaatacggc
     1441 tatttcgcgc gcctaaatta tcgtgacgga cgccaccgcg ttatccgtgg cggcgattta
     1501 ggcatcaacc ctggaacatc agcagaacta tcgaaggaca agggctacgc caaattcatg
     1561 ttgaaagttt taggcctcaa cacaccgcaa ggacagacgt tcttgctgcc agattgggcg
     1621 caaaaagtta ctgccaaccc taaacaacat gacaacccaa atattcgaac caccgaggct
     1681 gcgtatccat atattgaggg gaattttggc taccctgtat acgttaaacc agtcgatggt
     1741 tcgcaaggta ctaacatata tcgagtcgat agccggcaag aacttggcgg ggtatttgca
     1801 gcctatgctg agcgtggcgt gcgagtcgcg ttaattgaaa agcctatcga tctgccagac
     1861 taccgtatcg tcgtgctcga cggcaagctc attagcgcat accgccgcga cccgctggca
     1921 gttatcggtg atggcaagca aaccgtccgc gagctggtcc acgccgttca gacacagtac
     1981 caagatggtg gccgcgatac gaggctaagt ccagacgacg aacgtttagt agctactctt
     2041 ggtaaggccg gcctgacact cgaaagcgtc ttgcccgctg gcaatagaca gacactttta
     2101 cccatatcga acctgtcagc ggggggtaca tcggaagacg taagcgacag tattagcccg
     2161 cgttgggttg aattagccgc atctgtcgct agtaacttta atctacgctt atgcgggctt
     2221 gacctagcat gcagcgatat cacaaatagc aaagcggatt acagcattct tgaagtgaac
     2281 gccgcccccg ggctagacca ctacgctgca tccggagaag ctcagcagca aatcgtcgat
     2341 gatttatata ctcgtgtagt taataatccc tgacacagga atcgcaatgc tcgtaacagc
     2401 cataaaaaca cccaaaatca cagccggcag tattgatcta gtaaacctgc ttgatcaagc
     2461 gctccctgta cttaccgacg gctccattgt ggttataacc tccaaaatcg tatcgctttg
     2521 cgaaaatagc gttattccaa tcggctcaat agacaaggaa cagcttatcg ccgaacaagc
     2581 cgaactctac ttaccgcctt cttttagcag atataaccat cacttcacta ttatcggcaa
     2641 cacgctcgtt ggcgttgctg gaattgacga gtcaaacggc gacggcaatt acatattgtg
     2701 gccaagagac gcgcaa
//
LOCUS       DATNGP010000206         3029 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_1178069, whole genome shotgun sequence.
ACCESSION   DATNGP010000206 DATNGP010000000
VERSION     DATNGP010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3029
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1178069"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /locus_tag="VN778_05340"
     CDS             complement(<1..288)
                     /locus_tag="VN778_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008578963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXR50420.1"
                     /translation="MDKRPLAERLRPETLDDIVGQEHLVGAGKIIHEIITHKQPTSLI
                     LWGPPGSGKTTLARIMARETGAEFVELSAVTAGKADVVKVIERAEQNQRLGM"
     gene            complement(327..1109)
                     /locus_tag="VN778_05345"
     CDS             complement(327..1109)
                     /locus_tag="VN778_05345"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXR50421.1"
                     /translation="MFVPTIIAARNEAGHIGHTLEALARQAQRVEPIVVINSCTDRTA
                     DIAQSAGAIVLESPEGKMPALQEGLHYLGRRALEPLLILDADSRPFTRHWSRCLTHEL
                     GNMPAEHPRIAWGPYIFLGDINPAMGLFFSATAMRVSWADRHDSDPRAIRGGNTSIAL
                     TKAPVLEEFLAMPNYWPREDVAIFDNIMQHEGVHKVIFHPDAWATTSGARLATNIKRI
                     ITKCQNPTKVYDESYEREAPPGSTPYIATHSKRLNKNPGASH"
     gene            1243..2355
                     /locus_tag="VN778_05350"
     CDS             1243..2355
                     /locus_tag="VN778_05350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50422.1"
                     /translation="MYITPEVESALPATVQAFETVNERFAADPEAHLKKLEESLFSFK
                     STTGARIDCSIIPGQGKEMLMMWAPFSDGQPKSDAATIFQYISDDTTSKLTAAPNSWN
                     QTTKSGVVSELLKAVNREMTVLTIYSPLPSFPNSAYTPDEYLKIRRGDFTPSTRIAIE
                     AIEEVQDRLHGKKSETQFEAVHLHGASLGASSAVGVANGLVRIAAHAVRSVTAQELIV
                     APRNVFPELAARFTVKDPVGEPSAYIAADTSWPVIAEPLIRQQIDGRGNEPMMFARML
                     QGMSKISRLKGLTRPGRNMTPHLIENLVASGVMVTIPLAENSGLTHDTPDYLPRSGED
                     IIKVRATEGQRTSHLIDEYVALTSLLAVMNIRRASR"
     gene            2407..>3029
                     /locus_tag="VN778_05355"
     CDS             2407..>3029
                     /locus_tag="VN778_05355"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018180342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HXR50423.1"
                     /translation="MNEFGFKVNHRLGRGTLARTGTITTPHGEIQTPAFVVVGTKATI
                     KALTPEQVRGVHAQAVLANAYHLYLQPGHKLVEQAGGLSKFMNWPGPTFTDSGGFQVL
                     SLGSGYKKVIDMVGGEVEDVTLPKKGERKAFVDDDGVTFYSHLDGSEHRFTAELSMQI
                     QHGLGADIMFAFDELTSLIDPYEYQVESLARTHAWAERCLLELERLRT"
BASE COUNT          699 a          767 c          803 g          760 t
ORIGIN      
        1 cataccgagc cgctgattct gctcggcccg ctcaatcact ttcacaacat cggctttccc
       61 ggcagtcacc gcacttagct ccacgaattc cgcgcccgtc tcccgcgcca tgatccgcgc
      121 tagcgtcgtc ttgccgctcc ccggcggccc ccacaaaatc aagcttgtcg gctgcttgtg
      181 agtaataatc tcatgaatga tcttgccggc acccaccaaa tgttcttgtc cgacaatatc
      241 gtccagagtc tcgggcctaa gccgctccgc cagtggtctc ttatccataa tgtaattata
      301 cccaagtctc tgcggcaggt tgcaagctaa tgacttgcgc ccggattttt gttgagccgt
      361 ttggaatgag ttgcaatgta cggtgttgag ccgggcgggg cttctcgttc gtacgattcg
      421 tcatatactt ttgtggggtt ttggcacttt gtaatgattc tttttatatt agttgctaac
      481 cgggctccgg aggttgttgc ccaggcatct gggtgaaaga taactttgtg cacgccctca
      541 tgctgcatta tgttgtcaaa gattgctacg tcttcacgtg gccaataatt aggcatagca
      601 agaaattctt cgaggaccgg ggccttagta agcgcaatac tggtattgcc tccccttatg
      661 gcgcgtgggt cgctgtcgtg acgatctgcc caagaaaccc gcattgcagt ggcgctaaag
      721 aaaagtccca tagcgggatt aatatcccca agaaatatgt atggtcccca ggctatacga
      781 ggatgttctg ctggcatatt cccaagctca tgggttaagc accgactcca gtgacgtgtg
      841 aaagggcggc tatcggcatc aagtatgagt aagggctcca aagctcgcct gccgagataa
      901 tgcaaaccct cctgcaaggc cggcatttta ccttccgggc tttccagtac tatggcgcca
      961 gcagattggg cgatgtccgc agtcctgtcg gtacagctat ttatcaccac aattggttct
     1021 accctctggg cttgccgtgc tagcgcctca agcgtatgcc caatatggcc agcttcatta
     1081 cgagccgcaa tgattgttgg gacaaacata ggtataattt aagaacaatt tttctgttct
     1141 gtcgaatttt cgtttacaag ttggacaaat catgttatct tagtccccat gattagagtt
     1201 gaacttcctt ctgaggtatg ggtaaaacct accgaaaagc ttgtgtatat aacgcccgag
     1261 gttgaatcgg cccttccggc gaccgttcag gcatttgaga cggtaaatga gcgcttcgct
     1321 gccgatccag aagcccatct taaaaagctg gaggaaagtc tcttctcctt taagtcaact
     1381 accggcgctc gcatagactg ctcgattata cctgggcagg gcaaagaaat gttaatgatg
     1441 tgggcgccgt tttctgatgg tcaacccaag agcgacgcag ccaccatctt ccaatatatt
     1501 tcagatgata ctacgtccaa gcttacagcg gcgcccaata gctggaatca aacgacaaag
     1561 tcaggcgtag tttcggaatt gctgaaagct gttaaccggg aaatgaccgt cctaacgatt
     1621 tatagcccgt tgccgagctt ccccaatagt gcctacaccc ctgatgagta tctgaaaata
     1681 agacgcggtg attttacacc gtcaacccgc atagcgattg aagcgattga ggaagtgcaa
     1741 gatcgcttgc atggtaaaaa gagtgaaacg cagttcgaag cagtacacct ccacggtgct
     1801 tcgcttggag caagctcagc agtaggtgtg gccaatggct tggtgcggat tgcggcccat
     1861 gctgtccgca gtgtgacagc tcaagagtta atagttgctc ctcgcaatgt ttttcctgaa
     1921 cttgcggctc gctttacggt caaggatccg gtcggcgaac cttcagctta cattgctgct
     1981 gatacaagct ggccggtaat tgctgaaccc ctcattcgcc aacaaataga cgggcgtggc
     2041 aatgagccga tgatgtttgc ccggatgttg caagggatgt ccaagatatc tcgacttaaa
     2101 ggcctgactc gccccgggcg taatatgacc cctcatctta ttgaaaactt ggttgcttcg
     2161 ggcgtgatgg tcaccattcc tttagccgaa aacagtgggc taacccacga tacgcctgat
     2221 tatttgccgc gcagtggcga agatataatt aaggtcagag ccaccgaagg acaacgtact
     2281 tcgcacctaa ttgatgaata cgtagcatta acatctcttt tggcggtcat gaatatccgg
     2341 cgggcgagcc ggtaatctgc ccccaattag ttttcggcga gtacgatttc tcgtatactt
     2401 catcaaatga atgaatttgg tttcaaggtt aaccaccgcc tagggcgcgg tactctggcc
     2461 cgcaccggca cgatcaccac gccgcacggt gagatccaaa cgccagcgtt tgtagttgtg
     2521 gggaccaagg cgaccatcaa ggcgctgacg ccggagcagg tgaggggcgt gcatgcccag
     2581 gcagtgctgg caaatgccta ccacctgtat ttgcagccag ggcataagct ggtggagcag
     2641 gcgggcgggc tgagtaagtt tatgaactgg ccggggccga cgtttacaga ctcgggcggc
     2701 ttccaggtgc tcagcctggg tagcggctat aaaaaggtta ttgatatggt tggtggcgaa
     2761 gtcgaggatg tgacgctgcc caaaaaaggt gaacgcaagg cgttcgtgga tgatgatggc
     2821 gtgacgtttt attcgcattt ggatggcagc gagcatcgtt tcacggctga gttgtcgatg
     2881 cagatccagc acggcttagg tgccgatatc atgtttgctt ttgatgagct gacgtcgctc
     2941 atcgaccctt atgaatatca ggtggagtcg ctggcgcgga cgcatgcttg ggcggagcgc
     3001 tgcttactgg agctggagcg cttacggac
//
LOCUS       DATNGP010000207         4099 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_4576025, whole genome shotgun sequence.
ACCESSION   DATNGP010000207 DATNGP010000000
VERSION     DATNGP010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4099
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4576025"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="VN778_05360"
     CDS             complement(<1..295)
                     /locus_tag="VN778_05360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50424.1"
                     /translation="MQPSYPPAPMTPQPNDYSFITNPAPPPKPGLPLPGNSFTARLVI
                     ALGGLLVLLILFAIIKSVLGGSSNQPALLSVAQDQQEINHLTANAAQSSQSQLN"
     gene            367..2130
                     /gene="dnaG"
                     /locus_tag="VN778_05365"
     CDS             367..2130
                     /gene="dnaG"
                     /locus_tag="VN778_05365"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HXR50425.1"
                     /translation="MDAVDEVKSRLSIEDVVGEYVQLKRAGRNWRGLSPFTSEKTPSF
                     MVSPEKQIWHDFSSGKVGNMFSFVMEVEGLDFKGALELLARKAGIDIEQYRSSPRRGG
                     PDKERLYELLELATKFYQTQFSQHKTALTYVLKQRGFTKETALLFRIGYSPNTGLALA
                     TFAKSKGFTDTEIKQAGLSAQAYRGGVQDMFRGRLMIPLQDPQGRVIGFTARLLEDDA
                     RAPKYINTPQTVLYDKSRHIYGLHLGKEAIRKGQFAVLVEGNLDVIASHQAGVRQVVA
                     TAGTALTEPQLKALSRFTGDIRLSFDADKAGVAATERAIPIASRVKVSLSIIDIPSGK
                     DPDELIKQDPALWQQAIEKHQYALDWLMVRYEKLLDVASAPGKRQFSDVLLPIVRGLS
                     DPVERDHYLNAIAERIGVSKEALDQKLQKTAGPEVSERRRRIKVDPKQLNKVAVENQK
                     TQDNFLSLMLMRQTLREFLELVTEDMLYTPEGKALLKFLKKHLDFDGKNARMVQSLAE
                     YGKILALLYEELYQGLELNELHYEAARLQARLVEQFVKTEKSKLAEQLHDAPTNEARD
                     LLGQVKRYDALLNQVKGAANG"
     gene            2123..3256
                     /gene="rpoD"
                     /locus_tag="VN778_05370"
     CDS             2123..3256
                     /gene="rpoD"
                     /locus_tag="VN778_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HXR50426.1"
                     /translation="MAKKEKNAKPAEEPSFEQKRQDLIVKAKKDGHISQRDIFAVIPE
                     TPENAEVLDTLYTELADLNIEITTTDPDVANFTDEWAEDEAEEEELATDTAVYLDDDV
                     ADDSVRLYLREIGKIPLLSAEEELALAQRVVAGDKDAKDKMAEANMRLVVSIAKRYVG
                     RGLDLLDLIQEGNTGLLRAVEKFDPDKGFKFSTYATWWIRQAITRAIADQARTIRIPV
                     HMVETINKLLRTQRRLTQELNREPTNDEIAKAMEIDVDKVEHIMKIKQDISSLDASIR
                     DDEEDSVLADFIEDEDTVSPEESATGQLLKEQVKDMLAALTEREQKILKLRFGLEDGK
                     SHTLEEVGQEFSVTRERIRQIEAKALAKLRKHRDAKKLHDYIK"
     gene            complement(3259..3831)
                     /locus_tag="VN778_05375"
     CDS             complement(3259..3831)
                     /locus_tag="VN778_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXR50427.1"
                     /translation="MQDKKVIVFVGMPGAGKSVCVEYLKKKGLPYVYFGGITLDEIKR
                     RGMEVNEASEKFVREDIRAKEGKGAYAVRIIRQIEELFDQGHNKVVVDGLYSWTEYKI
                     FKESFGDNVIIIAIASPRKFRHQRLATRVVRPLTEEEANARDYAEIENLEKGGPIANA
                     DYFLANETSVEELETDLGKLLPKIGLELPK"
     gene            complement(3916..>4099)
                     /locus_tag="VN778_05380"
     CDS             complement(3916..>4099)
                     /locus_tag="VN778_05380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50428.1"
                     /translation="SGDELLIEVFEGSGRVIRIQDGEVAESFRQLWHLIDKQTRSAPG
                     YQTYSPSAKRQLPSAN"
BASE COUNT         1039 a         1010 c         1148 g          902 t
ORIGIN      
        1 tgttaagctg actctggctg ctttgggccg cgttggctgt caggtggttt atttcctgct
       61 ggtcttgcgc gacgctcaag agggctggct gatttgagct gccaccgagc acacttttga
      121 taattgcgaa caggataagc agtactaaca aaccgccaag cgcaatgact aagcgggcag
      181 taaatgaatt acctggcaag ggcaaacctg gctttggtgg cggcgccggg ttcgtgataa
      241 acgaataatc attcggctgc ggcgtcatag gtgccggcgg ataagacggt tgcataggtt
      301 taattaaacc ataagcttat cgtcttgggt aaggcttgac aaatttagcg tacaatagag
      361 gtacatatgg atgctgtgga tgaggtgaag agccgcttaa gtattgaaga tgtcgttggc
      421 gagtatgtcc agctgaaacg ggcggggcgg aactggcgcg ggttgagccc ctttaccagc
      481 gaaaagacgc cgagcttcat ggttagtccc gagaagcaga tttggcatga tttcagttcg
      541 ggcaaggttg gcaatatgtt cagcttcgtt atggaggtgg aagggctgga tttcaagggt
      601 gcactggaac tgttggcccg caaagccggc atcgatatcg aacaataccg gtcgtcgccg
      661 cgccggggcg gaccggataa agagcgactg tacgagctac tggagctggc gactaaattt
      721 tatcagacgc agttttcgca gcacaaaaca gctttgacgt acgttctcaa acaacgtggg
      781 tttacgaagg aaacggcgct cttgttccgt atcggctact ctccgaatac cggtttggca
      841 ctcgcgacct tcgcgaaaag caagggattt acggatacgg agataaagca ggccggcttg
      901 agtgcccagg cataccgtgg gggggttcag gatatgttcc gagggcgctt gatgattcct
      961 ctgcaagatc cgcaggggcg cgtaattggc ttcacggctc gcttgctgga agacgacgca
     1021 cgagcaccga agtacatcaa cacgccgcag accgtgcttt acgataagag tcggcatatt
     1081 tatgggttgc atttgggcaa ggaggcgatt cgtaaaggtc agtttgcggt cttggtcgaa
     1141 ggaaatttgg atgtcatcgc ctcgcatcaa gccggtgtca ggcaggtggt agcgacggca
     1201 gggacggcgc tgaccgagcc acagttgaaa gcgttgagcc ggtttaccgg cgatattcgc
     1261 ttgagctttg atgccgataa agcaggggtg gcggcgacgg aacgggcgat tccgattgcc
     1321 tcccgagtca aagtctcttt aagtatcatc gacattccgt cggggaaaga tccggacgag
     1381 ctcatcaagc aagaccctgc gctgtggcag caggcgatcg agaagcatca atatgccctg
     1441 gattggttga tggtgcgcta cgaaaagctg ctggacgttg ctagcgcgcc cggcaagcgg
     1501 caatttagtg atgtcttgct gcccatcgtc cgcggcttgt ctgatccggt cgagcgcgac
     1561 cactacctga atgcgatcgc tgaacgcatt ggtgtgagta aagaagcttt ggatcaaaag
     1621 ctccaaaaaa cggcaggacc agaggtaagc gagcgtcgcc ggcgcattaa agtagatccc
     1681 aagcagttga ataaagtagc cgtggaaaac caaaaaacac aggataattt tctgagcctc
     1741 atgctgatgc gccagacgtt gcgcgaattc ctcgagcttg ttaccgaaga tatgctatac
     1801 actcccgagg gcaaagcact actcaaattt ttgaaaaagc atctggattt cgatggcaag
     1861 aatgcgcgga tggtgcaaag cttggccgaa tatggtaaaa tattagcatt gctatatgag
     1921 gaactctacc agggcctcga gcttaatgag cttcactacg aagccgctcg gttgcaggca
     1981 cggttggtgg aacaatttgt aaaaactgaa aaaagtaaat tggctgaaca actgcatgat
     2041 gcgccgacga acgaggcgcg ggatttgctt ggccaagtaa aacgctacga cgcattactg
     2101 aatcaagtga aaggagctgc taatggctaa aaaagagaag aatgctaaac cggcggagga
     2161 gccgtcgttt gaacagaaaa ggcaagatct gatcgtcaaa gcgaagaagg atgggcacat
     2221 ttctcaacgc gacatcttcg ctgttatacc ggagacgccc gagaacgccg aagtcctgga
     2281 cacgctctac accgagctgg ccgatttgaa tattgaaatc accacgaccg acccggatgt
     2341 ggctaacttt actgatgagt gggccgaaga tgaggccgag gaagaagaat tagcgacaga
     2401 caccgccgtt tatctcgacg atgacgtggc tgacgactcg gtccgtctgt acctgcgcga
     2461 gatcggcaaa attccgctat tgagcgctga ggaagagttg gcgctcgctc agcgcgtggt
     2521 cgccggcgac aaagatgcca aagacaagat ggcggaagcc aacatgcgcc tggtagtctc
     2581 gatcgccaag cgctatgtcg gccgcggcct ggaccttttg gatctgatcc aggaaggtaa
     2641 caccggctta ctgcgtgccg tcgagaaatt cgatcccgac aagggcttta agttctcgac
     2701 ttacgccacc tggtggattc gccaagctat tactcgggct attgctgacc aggcccgcac
     2761 cattcgtatc ccggtacaca tggtcgaaac catcaacaag ctgctgcgca cccagcgccg
     2821 cctgacccag gaactgaacc gtgagccgac caacgatgag attgccaaag ccatggagat
     2881 cgatgtcgat aaggtcgaac acatcatgaa gattaagcag gatatttcgt ctttagacgc
     2941 ttcgatacgt gacgacgaag aggattcggt gctagctgac tttatcgaag atgaggacac
     3001 tgtatcaccg gaagagtctg caacggggca attactgaaa gagcaagtca aagatatgtt
     3061 ggcggctctt accgagcgcg aacagaagat cctgaaactg cgtttcggcc tggaagacgg
     3121 caaatcacac acgctggaag aagtcggcca ggaattctct gtcacccgcg agcgcatccg
     3181 tcagatcgaa gctaaagcac tcgccaagct gcgcaagcac cgcgatgcca aaaagctaca
     3241 cgattacata aaataagtct acttgggaag ttcaaggcca atcttaggca ggagtttccc
     3301 gaggtcggtt tccaactcct cgacggaagt ttcattagcc aggaagtaat cggcattggc
     3361 gatgggtccg cctttttcta gattttcgat ctcggcgtag tcgcgagcgt tggcttcctc
     3421 ttcggtgagc ggccgaacga cgcgagtggc gagccgttga tgacgaaatt tgcgaggcga
     3481 agcgatggcg atgatgatga cattgtcgcc gaagctttcc ttgaagattt tgtactcggt
     3541 ccagctataa agcccgtcta cgaccacctt attatggccc tggtcaaaca gctcctcaat
     3601 ctgccggata atccgcactg cgtaagcgcc tttgccttct ttagcccgga tgtcttcgcg
     3661 gacgaacttc tcgctcgctt cgttaacttc catgccgcgc cgtttgattt catcgagcgt
     3721 gatgccgcca aaatagacat aaggtaagcc cttctttttc aggtactcca cgcacacact
     3781 cttgccagcc cccggcatgc cgacgaaaac gataactttc ttgtcttgca tcaggcttat
     3841 tgtacaagac gaaagccggc cctgaaaccc tagaagcctt ctggctacgc accgggcaga
     3901 tgctcggccc ctaagctaat tagcactcgg caattgccgt ttggcacttg gagaataggt
     3961 ctgataaccg ggcgcagaac gggtttgctt atcgatgagg tgccatagtt gacgaaagga
     4021 ttcagccact tctccatcct gaatccgaat cacacgtcca ctaccctcaa atacctctat
     4081 gagcagttca tcaccacta
//
LOCUS       DATNGP010000208         2094 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_2732444, whole genome shotgun sequence.
ACCESSION   DATNGP010000208 DATNGP010000000
VERSION     DATNGP010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2732444"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="VN778_05385"
     CDS             complement(<1..200)
                     /locus_tag="VN778_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003917752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXR50429.1"
                     /translation="MQKRQKFVGLSDAERSEIATLLRRGCSLREIAQALGRSPNTVSY
                     ELRQNSVGGVYDPLKAKQKSRV"
     gene            345..770
                     /locus_tag="VN778_05390"
     CDS             345..770
                     /locus_tag="VN778_05390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50430.1"
                     /translation="MSKAIYTIVDETFGPMPDDDPTTPVTPVLRLGGLLALMRRVRPD
                     DNSQYDWINYVDEKGEPQRAQATGFVSVVQAQLRPKEDAQVTSFRVVALIGEQVLVHY
                     QGNTFRFMNAADYELAQAVLGLHDELQGRNWQVPREAQK"
     gene            complement(774..>2094)
                     /locus_tag="VN778_05395"
     CDS             complement(774..>2094)
                     /locus_tag="VN778_05395"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HXR50431.1"
                     /translation="VWQAVMHIVPSGKQVAAPDVGKLAIGRSLVASLTAQIYQIAALA
                     EQITGKNKTEAASLQANFVASNLPLPLFVLNAEQTIIFANSSAATYLGRESQDIIGQN
                     VYSILNMLFPNEETFSVWFDKVRSTAVTASSYWERVRIEVSDNQPMRMFDLAAYYNKG
                     NSTGYETMLVMFDHTKTYSQDDQAVSLIALGMHELRTPLALLRGYIEAFDEELTGKLS
                     PELDGFMSKMKATAQQLNAFVNNILNVARVDNDQMPLKLHEENWPVVLKEAVDMMSLR
                     AQVRGITLKLNVADGLPTVGADRVSMQEVVANLIDNAIKYSGQSKEILIDAHMNAEGY
                     VETTVQDKGVGVPASIMPNLFTKFYRDHRNRAQIGGTGLGLYLCKAFVSAHGGNIWVK
                     SREGQGSTFGFTLVPFAHLADEIKNNANQEITRSAHGWIKNHSLYRR"
BASE COUNT          465 a          540 c          577 g          512 t
ORIGIN      
        1 ctgactcgac ttttctgctt ggccttgagc gggtcgtaga caccgccaac actgttctgc
       61 cgtagctcgt agctcacggt attgggacta cgccccaagg cctgagctat ctcccgcagg
      121 ctacaaccac gccgcaacaa ggtggctatt tcgctccgct cagcgtcgct caaaccaaca
      181 aatttctgcc tcttttgcat ccccttactg tatctgaggt tttagcaggg tgtcctaata
      241 tattccggaa agttcgtaga tcactactag agacgactct tggacagtat gccgttgaca
      301 gcaatgaagg cgcactgaac gacgcaccgt ataacgcagc atccgtgagt aaagctatat
      361 acacgatcgt cgatgaaact tttggtccaa tgccggacga tgatccgact acaccagtga
      421 cgccggtgct acggttgggc ggactactgg cgctcatgag aagggtacgt cctgacgata
      481 atagtcagta tgattggatc aactatgtcg acgaaaaagg agagccgcag cgtgcgcaag
      541 caacaggatt cgtaagcgtt gtccaagcac aacttcgccc caaggaggac gcgcaagtca
      601 catcgtttcg ggtggtggct ttgataggcg agcaggtact cgtacactac caaggcaata
      661 cgttccggtt tatgaacgct gccgactatg aattagccca agctgttctg ggcctacacg
      721 atgaactcca gggtagaaac tggcaagtac cacgagaagc acagaagtag tgtttagcgg
      781 cggtacagcg agtggttttt gatccagccg tgagcacttc gagtaatttc ctggttagcg
      841 ttgtttttga tctcgtcagc caggtgggcg aacggtacta acgtgaagcc aaaagtgctc
      901 ccctggcctt cacggctctt gacccagatg ttgccaccgt gggcggagac gaatgcttta
      961 cagaggtata atcctagacc cgtgccgcca atctgagctc ggttacggtg gtcgcggtag
     1021 aacttagtaa acagattggg catgatgctg gcgggcacgc cgacgccctt gtcctggacg
     1081 gtggtttcga cgtaaccttc ggcgttcatg tgcgcatcga tcagaatttc tttactttgg
     1141 ccgctatact tgatggcgtt gtcgatgaga ttagcgacga cctcctgcat actgacgcgg
     1201 tcggcaccca ccgtgggcaa tccatcggca acattcagct tcaacgtaat gccacggact
     1261 tgggcgcgga gtgacatcat gtcgacggct tctttgagca ctactggcca attttcttcg
     1321 tggagcttca gcggcatctg gtcattatcg acgcgtgcga cgtttaagat gttattgacg
     1381 aaggcgttga gctgctgggc ggtagccttc attttagaca taaagccgtc gagttcgggc
     1441 gatagtttcc ccgttagttc ttcgtcaaac gcttcgatat aaccccggag cagtgccagc
     1501 ggcgtgcgca gttcgtgcat accgagcgcg atcaggctga ctgcctggtc gtcctggctg
     1561 taggttttgg tatggtcgaa catgacgagc atggtctcgt aaccggtcga attgcccttg
     1621 ttgtagtatg ccgccaagtc gaacatgcgc atgggttgat tgtcactgac ctcgatccgc
     1681 acgcgctccc agtacgagct ggcggtgacg gccgtggaac ggactttatc gaaccagaca
     1741 ctgaatgttt cttcatttgg aaacagcatg ttcaggatag aatagacgtt ttggccgatt
     1801 atgtcttggc tttcccgtcc cagatacgtc gcagccgagc tgttagcaaa aatgattgtt
     1861 tgctcggcgt tcaatacgaa tagcggcagg ggcaaattac tcgctacgaa gttagcctgc
     1921 aggctagcag cttcggtttt attcttgccc gtaatttgtt cggccaacgc ggcaatctgg
     1981 tagatctggg ccgtgaggct ggcgaccaaa gagcggccga tggctaattt gccgacatcc
     2041 ggcgcggcaa cttgcttacc cgaaggcacg atatgcatga ctgcttgcca tacg
//
LOCUS       DATNGP010000209         4489 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_4278123, whole genome shotgun sequence.
ACCESSION   DATNGP010000209 DATNGP010000000
VERSION     DATNGP010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4489
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4278123"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..272
                     /locus_tag="VN778_05400"
     CDS             <1..272
                     /locus_tag="VN778_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015535559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HXR50432.1"
                     /translation="LTRKTHPLYRKQYTVSKKFMAHDEKNEAQVGDKVAIVETRPVSA
                     RKRFRLSEILEKPALREDTLAATKSEDEGKKPTKASAAETKEEAK"
     gene            272..640
                     /gene="rplN"
                     /locus_tag="VN778_05405"
     CDS             272..640
                     /gene="rplN"
                     /locus_tag="VN778_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003247583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HXR50433.1"
                     /translation="MIQQESRLVVADNSGAKEILCIRVLGGTRRRYAKVGDVIVATVK
                     DATPNGTVKKKTVVKAVVVRTRNQIRRKDGSTIKFDDNAAVIIGDDKQPRATRIFGPV
                     PRELRDQGYARIISLAPEVL"
     gene            640..978
                     /gene="rplX"
                     /locus_tag="VN778_05410"
     CDS             640..978
                     /gene="rplX"
                     /locus_tag="VN778_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014465726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HXR50434.1"
                     /translation="MTTPSKLYKIRLKKGDLVVVRAGKYKGKTGKVTATHPRENKVTV
                     AGINIVKKHLKPNQQHPQGTILEITQPIWVSKVSIVEPTSKKPSRIGYRLDKDGNKTR
                     VFKRTGKEIK"
     gene            978..1568
                     /gene="rplE"
                     /locus_tag="VN778_05415"
     CDS             978..1568
                     /gene="rplE"
                     /locus_tag="VN778_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008578361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HXR50435.1"
                     /translation="MATATVKPAKTLTTAARLRVQYNETLAKELQKELGLKNIHQAPR
                     LEKIIVNSGLGRAKDDKKILEVATNTLCKITGQQPVETIAKNSIAGFKLREGNKVGLK
                     VTLRGERMYEFMDRVITVVLPRLRDFHGVSAKAFDRQGNYAIGFTDQSVFPELSFEET
                     TTAHGLQMIFVISAQEKEHARALLEKFGMPFEKEAK"
     gene            1570..1839
                     /gene="rpsN"
                     /locus_tag="VN778_05420"
     CDS             1570..1839
                     /gene="rpsN"
                     /locus_tag="VN778_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HXR50436.1"
                     /translation="MAKKSIVARDAKRIRMIEKYAEKRKELKELGDQEGLAKLPRNSS
                     PTRRTNRCVETGRSRGYMRQFGLSRISFREHASKGEIPGVTKSSW"
     gene            1849..2250
                     /gene="rpsH"
                     /locus_tag="VN778_05425"
     CDS             1849..2250
                     /gene="rpsH"
                     /locus_tag="VN778_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008578362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HXR50437.1"
                     /translation="MSTTTTDPISDMLTRIRNAIAVRKSEVTLPHSNIKESVARLLQE
                     SNFIDGVSVEDAPVGKTLKVKIYDTDTNPRITEIVRLSKPGRRYYVNAREIPVVKRGR
                     GVVIISTSKGLMTGDQAKTERVGGELICKVY"
     gene            2269..2808
                     /gene="rplF"
                     /locus_tag="VN778_05430"
     CDS             2269..2808
                     /gene="rplF"
                     /locus_tag="VN778_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HXR50438.1"
                     /translation="MSRVGKLPIAIPSGVTITVEPAEITIAGTKGTLKQFTMPDITVK
                     QEGEDLIIARANDEPKIRAKHGLMRALLNNMVTGVSQGFSKKLEINGVGYRIAQQGAD
                     LKLNLGFSHDVIYHVPQGITTAVEQNTITISGISKQQVGQVAAEIRALKKPEPYKGKG
                     IKYEGERIIRKSGKSGKEK"
     gene            2809..3168
                     /gene="rplR"
                     /locus_tag="VN778_05435"
     CDS             2809..3168
                     /gene="rplR"
                     /locus_tag="VN778_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HXR50439.1"
                     /translation="MSKQLAHKLKNRALRKNRIRSKISGTAARPRLSIFVSNIHITAQ
                     LIDDTAGKTLGYVTTVGQKSLKGTMTEKADWVGTEIAKQAKAAKVKAIVFDRGGKLYH
                     GRVAALADAARKAGLEF"
     gene            3171..3773
                     /gene="rpsE"
                     /locus_tag="VN778_05440"
     CDS             3171..3773
                     /gene="rpsE"
                     /locus_tag="VN778_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015642153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HXR50440.1"
                     /translation="MADTPAQTRERRPRRPEVIPEEKQFDERTLHIDRVARVVKGGRR
                     FRFRALVVVGDRKQKVGIGTAKGADVTAAVTKATEVAKKNMISVSLYKGTLPHEAEAK
                     VSGAHILVKPASAGTGLIAGGVVRSILEVAGVHNALSKSLGSSNKTNTAYATIAALDS
                     LVPANKWVTAKAATKPKAVADTKKPTTAKKAPTKKTTKKA"
     gene            3777..4253
                     /gene="rplO"
                     /locus_tag="VN778_05445"
     CDS             3777..4253
                     /gene="rplO"
                     /locus_tag="VN778_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010164604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HXR50441.1"
                     /translation="MKYHELQTTKQKSATRVGRGIAAGKGKTAGRGTKGQGARTGSSA
                     KPGFAGGSNPLMQKLPKLPGFRSHRPKAETVFTGQLEQFGSKTVDAQVLAEAQLISSP
                     YVTVKLLSKGDLTKKVTVKLPGASESAIAAIQSAGGSFEKTARLSRPKTSTKKTKK"
BASE COUNT         1286 a         1118 c         1156 g          929 t
ORIGIN      
        1 tacttacccg taagacgcac ccgctatatc gcaagcaata cacagtgagc aagaagttta
       61 tggcgcacga cgagaaaaat gaagcacagg ttggtgacaa agtcgcgatt gtcgaaacgc
      121 ggccagttag cgcccgcaag cgtttcaggc tcagtgaaat tttggagaag ccagcgctac
      181 gcgaagatac gctcgctgcg actaaatctg aagatgaagg taaaaagccc actaaggcta
      241 gtgctgctga gactaaagag gaggccaagt aatgattcag caagaatcac gactggttgt
      301 ggcagataat agcggagcca aggaaattct ctgcatccgt gtcctcggcg gtactcggcg
      361 ccgctatgcc aaggttgggg atgtgatcgt ggcaaccgta aaagatgcta ccccaaacgg
      421 caccgtcaag aagaagacgg tggtgaaagc cgtcgtcgta cgcactcgca atcaaatccg
      481 ccgcaaagac ggttcaacca ttaagtttga cgataatgcc gctgtaatca tcggcgatga
      541 caaacaacca cgcgccaccc gtattttcgg cccagttcca cgcgagttgc gcgatcaagg
      601 ttatgccaga atcatttcac tggctccgga ggtactataa tgacaactcc atcgaagctg
      661 tataaaatcc gcctgaaaaa aggtgatctg gttgtcgtgc gtgccggaaa atacaaaggc
      721 aaaaccggca aggtgacagc tacccacccg cgtgagaaca aagtgacggt tgccggcatc
      781 aatatcgtga aaaagcacct aaagcccaat caacagcacc ctcaaggcac tatcctggaa
      841 attactcagc ctatctgggt cagcaaagtt tctatcgtcg aaccgacctc taaaaagccc
      901 agccgtattg gctaccggct tgataaagat ggcaacaaga cccgcgtgtt taagcgaact
      961 ggaaaggaga tcaaataatg gccactgcaa ctgtaaaacc agctaaaacc ctcaccactg
     1021 ccgcccgtct ccgcgtccag tacaatgaga cgcttgccaa agaactgcag aaagagttgg
     1081 gactcaagaa tatccatcaa gcaccacgct tagagaaaat tattgttaat agcggtcttg
     1141 gtcgtgccaa agatgataag aaaattctgg aagtggctac taatactttg tgcaaaatca
     1201 ccggccagca gcctgtcgag accattgcta aaaattcaat tgccggcttt aagctgcgcg
     1261 aaggcaataa ggtcggactt aaagtgacat tgcgcggcga acgaatgtat gaattcatgg
     1321 accgtgtcat taccgttgta ctgcctcgtt tgcgtgactt tcacggggtg agcgccaagg
     1381 cgttcgaccg ccaaggcaac tatgccatcg gcttcaccga ccagtcagtt ttccccgaac
     1441 tgagctttga ggaaaccacc accgcccatg gcttgcaaat gatctttgtc atcagcgcgc
     1501 aagaaaaaga gcatgcccgc gctttactcg aaaaattcgg catgccattt gagaaggagg
     1561 ctaaataata tggctaaaaa atcaattgtg gctcgcgatg ctaaacgtat cagaatgatt
     1621 gagaaatatg ctgagaagcg taaggaactc aaagagctcg gcgaccaaga aggcttggct
     1681 aagttgccac gtaattcgtc accgacgcgc cgcactaata gatgcgttga aaccggccgc
     1741 tcgcgcggct atatgcgcca gtttggcctg agccgtatta gtttccgtga acatgctagc
     1801 aagggcgaga tccctggtgt aacgaagagt agttggtaag gagaaagcat gagtacaacc
     1861 acaaccgatc ccatttccga catgctaact cgtattcgta acgccattgc cgtgcgaaag
     1921 agtgaggtta cactgccaca ctccaatatt aaggagagtg tagctcgctt gctgcaagaa
     1981 agtaatttca tcgatggcgt ttccgtggaa gatgcgcccg tcggcaagac tttgaaggtc
     2041 aagatttacg acacggacac caacccgcgc attaccgaaa ttgtccgtct tagcaagccc
     2101 gggcgccgtt actatgtcaa tgccagggaa attccagtag tgaagcgtgg tcgcggtgtg
     2161 gttatcattt cgactagcaa aggcctgatg accggcgatc aagccaagac tgaacgtgtt
     2221 ggcggcgaat taatttgtaa ggtttattaa aggatagagg attaagatat gtctcgagta
     2281 ggaaaactgc cgatcgccat tccgagcggt gtgacaatca ctgtcgagcc tgcagaaatc
     2341 accattgccg ggactaaggg aacgcttaag cagttcacca tgcctgacat aacggtgaag
     2401 caagaaggtg aagatctgat tatcgcgcgt gctaatgacg agcccaaaat cagggctaaa
     2461 cacggactta tgcgcgcttt gctcaacaat atggtcaccg gtgtcagcca aggcttttcg
     2521 aaaaagctgg aaatcaacgg tgtcggttat cgtatcgcgc aacaaggtgc cgatctgaag
     2581 ttgaatctcg gtttttcgca cgatgtcatt taccacgtgc cgcaaggcat tactactgcg
     2641 gttgaacaga ataccatcac gattagcggc atcagtaagc agcaagtcgg ccaagtcgct
     2701 gccgaaatcc gagcgctgaa gaaacctgag ccttacaagg gtaaaggcat taaatacgaa
     2761 ggagagcgca ttattcgtaa atctggtaag agtgggaagg aaaagtaaat gagtaagcaa
     2821 ctagcccaca aattgaagaa tcgcgctttg cgcaagaatc gtatccgtag taagatcagc
     2881 ggtactgccg cccgaccgcg cctgagcata tttgtcagca atatacacat tacggcgcag
     2941 ctgattgatg acaccgctgg caagacgcta gggtatgtta ccaccgtcgg ccagaaatcc
     3001 cttaagggca ctatgactga aaaggctgat tgggttggta cggaaatcgc caagcaagcc
     3061 aaggctgcca aagtcaaggc tattgtgttt gatcgtggag gtaagttata tcacgggcgc
     3121 gtagctgcat tagccgacgc cgctcgtaaa gcaggattgg agttttaatc atggcagata
     3181 caccagcaca aacccgagaa cgtcgccccc gtcgcccgga agtcattccc gaagaaaagc
     3241 agtttgatga gcgcaccctg catattgacc gagtggcacg agtggttaaa ggtggccgtc
     3301 gcttccgctt ccgcgctctt gtcgtcgttg gcgaccgcaa gcaaaaagtt ggcatcggta
     3361 ctgcaaaagg tgcagacgta accgctgctg ttaccaaagc aactgaagtt gccaaaaaga
     3421 atatgataag cgtttcactg tacaaaggca ccttgccaca cgaggctgaa gctaaagtca
     3481 gcggcgcgca tatactggtg aaaccagcca gtgctggtac tggattgatt gctggcggag
     3541 tcgtccgctc cattttggag gtggctggcg tgcataatgc gctgagtaag tctttgggtt
     3601 catccaataa gactaatacc gcttacgcca caattgctgc gctcgacagt ttagtaccgg
     3661 ccaacaaatg ggtcacagct aaagcagcta ccaagccgaa ggctgtagcc gataccaaga
     3721 agcccactac tgctaagaaa gccccaacta agaagacaac gaaaaaggct taatctatga
     3781 aataccacga acttcaaact accaagcaaa aatcagccac tcgcgtcggc cgcggcattg
     3841 ctgccggtaa aggtaagact gccggccgcg gcactaaggg ccaaggcgcc cgcaccggct
     3901 cgagcgcaaa acctggtttt gcaggcggct ctaacccttt gatgcagaag ctaccgaaac
     3961 tcccgggctt ccgcagccac cgtccgaaag ccgaaacagt ctttacaggg caactcgagc
     4021 aattcggcag caagaccgtc gatgctcaag ttttagccga agcccagctt attagtagtc
     4081 catacgtaac tgtcaagttg ctctcaaaag gtgatttgac gaagaaggta acggtcaaac
     4141 taccaggtgc cagcgaaagc gccattgccg caatccagtc cgccggtggt tctttcgaaa
     4201 aaaccgcccg gcttagccgt cctaaaacct cgaccaaaaa aaccaaaaaa taattctgca
     4261 gcagccgcca gtgaagcagg gctatgcgcc cacgtactca tgctaaaata ggcctataga
     4321 tggagggtga gctatggctg ctgcagccaa aggtcttgag caggaattgc gggtggctct
     4381 agttggcgat gtgtgggagc atcttggcat tagctgcaag catgttttca ttgaagccga
     4441 gacgccgctg gagactaccg ccgctgaaaa agttgccgaa gccttacac
//
LOCUS       DATNGP010000210         7338 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_1816536, whole genome shotgun sequence.
ACCESSION   DATNGP010000210 DATNGP010000000
VERSION     DATNGP010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 7338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7338
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1816536"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..506)
                     /locus_tag="VN778_05450"
     CDS             complement(<1..506)
                     /locus_tag="VN778_05450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50442.1"
                     /translation="MKKPKKPKLPKPKLPKQLPELPKRPFKRSVKATEEKLNEALASV
                     PRITNETVAEHREDVLGGARKYIYPLRHSKHNIVRTSVTILVVGIVAFLLFCLLAIYK
                     LQSTSGFIYDVSLVVPFPVAKAGPAWVSYESYLFELRRNMHYYQTQQQANFSTRDGRS
                     QLTRLKEQA"
     gene            complement(484..1080)
                     /locus_tag="VN778_05455"
     CDS             complement(484..1080)
                     /locus_tag="VN778_05455"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HXR50443.1"
                     /translation="MAKEAKAAATQTRHKYFDDQFDDEEVLFVFHKHPIVMRKGLVLG
                     MLGPLAGVIPAAINPNLGFGIFFGGLAAGCLLGLLIFFPDWISWHFSVFIVTDQRFIQ
                     ITQKGLFHRAVADLGLSQIQSVNYEISGLQETLLGFGTIKMQTYVGDLVIRDVHHPAK
                     IQKQLLSILRDEGINAASPYSGDSSKLANEMHEETQEA"
     gene            complement(1103..1177)
                     /locus_tag="VN778_05460"
     tRNA            complement(1103..1177)
                     /locus_tag="VN778_05460"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1142..1144),aa:Arg,seq:cct)
     gene            complement(1222..1737)
                     /locus_tag="VN778_05465"
     CDS             complement(1222..1737)
                     /locus_tag="VN778_05465"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020524232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006165 - obsolete nucleoside diphosphate
                     phosphorylation [Evidence IEA];
                     GO_process: GO:0006183 - GTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006228 - UTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HXR50444.1"
                     /translation="MERTLIILKPDAVQRGIVGEIISRFERAGFKIVGTKMLDPDYDH
                     YYEHYEGIGTLKTRKGDEIFKSQLASMMEGPVIAMVLEGVDAVETVRKMVGPTEPKSA
                     QPGTIRGDFAHVSYGAAASVGKGVGNIIHASAEKDEADKEIAHWFAESEIHDYQSVHE
                     KFTQPRPKKAQ"
     gene            complement(1762..1977)
                     /locus_tag="VN778_05470"
     CDS             complement(1762..1977)
                     /locus_tag="VN778_05470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50445.1"
                     /translation="MYLYSPKMVTVQIPMERELRDALKRKALHLGFNSIQSFVRFWAQ
                     AEIEGRKVNLDDALWEPDIYKIKDVPE"
     gene            2254..3168
                     /gene="xerA"
                     /locus_tag="VN778_05475"
     CDS             2254..3168
                     /gene="xerA"
                     /locus_tag="VN778_05475"
                     /inference="COORDINATES: protein motif:HMM:NF040815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase/integron
                     integrase"
                     /protein_id="HXR50446.1"
                     /translation="MLFAKAKTDFLEYLEIEQNRSQKTIQNYDHYLTRLIDFAGDIQV
                     TDIDAELIRKWRLWLNRLGTNTSDELGKTTQNYHLIALRSFLKFCAKRDVPALTADKI
                     ELARTQRKQVTFLSEDELARLFAQPDLNTLSGLRDRAILELLFSSGLRVSELVGLDRD
                     HINLKRREFMVRGKGQKDRPIFISLEAATWIEHYLAKREDKTKPLFIRYSGTRAVDLS
                     GNFHRLTARSIQRLVARYALLAGITKHVSPHTLRHSFATDLLMNGADLRSVQALLGHS
                     NISTTQIYTHVTDPHLRAVHEKFHRHTD"
     gene            complement(3189..4355)
                     /locus_tag="VN778_05480"
     CDS             complement(3189..4355)
                     /locus_tag="VN778_05480"
                     /inference="COORDINATES: protein motif:HMM:NF025699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilX N-terminal domain-containing pilus assembly
                     protein"
                     /protein_id="HXR50447.1"
                     /translation="MPETKKIQENEQGFASIVVALILIIVLALLTIGFAQLARREQQN
                     ALDKQLSNQAYYAAESGVNDVVDGIKKGLVTEASLPMTSSKKCLTGGQLTSLSLNRNI
                     DTSHGVAYTCVMVDLTPPNLEFNIAPGNSQSSVFTPSPAPATPTIALTIQWGSGDATP
                     ALPHDSFFPPGQTPSFTPTNAWNAAPGHPPVLQFSITPFSSTSFDRNALINKTFTIYT
                     YPSKNSPGPNNPACPAAPNSTTCVAYDLAQQAQIVKGRCSTTFTPAPCSVTIYGIPYV
                     AGSSDMYLLHFMDFYDDASVYFNGVDQTGASFSFSGAQDTIDVTGQARNVLKRIQVRV
                     PANQSSSQAALSLPSAALQAGNICKHFQVDPTNGANFLNADGSLAGPGTACNLE"
     gene            complement(4375..5013)
                     /locus_tag="VN778_05485"
     CDS             complement(4375..5013)
                     /locus_tag="VN778_05485"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HXR50448.1"
                     /translation="MSRRRSVREQAGFTIIELMIATTVLSVILLMATAIMISIGNLFY
                     KGVSQARVQDNIRTITDDVSNHLRLGSAVANPTGSPLVRSGQSIYSLCIDTTRYSYVI
                     GMQIGAPLNHVLWRDTLATAGSCAPADLSTADPAATNGGVAGSGTELIGPKSRLTAFS
                     LSSTTTPYTINVGVAYGDPSLSPAGVCLGTSDTSDQFCSTATLTTTVDKRSN"
     gene            complement(5010..5513)
                     /locus_tag="VN778_05490"
     CDS             complement(5010..5513)
                     /locus_tag="VN778_05490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HXR50449.1"
                     /translation="MPAKLLKFKRTSEKGDTIVEVLIVLAVLGLAISICYATANRSLL
                     DARQAQENAVASELVQSQIEGLRVTMPSAVPGTAPMIAGQFCVVLNGGSYVVKPYISP
                     TPPDPSCALPFASSSSSISVIYCGTLVRNANCGILSDDTFVVTATWPDVIGGGNDTVS
                     QVYKVHP"
     gene            complement(5495..6337)
                     /locus_tag="VN778_05495"
     CDS             complement(5495..6337)
                     /locus_tag="VN778_05495"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HXR50450.1"
                     /translation="MMLGGKNKQPLGYTIIEVMIVLAVSGLMFVIAATFINGKQEKTA
                     FTAGVNEMASRLQDTIEQVVDGRFSDIPLTCAVGSSNLSFTSAGSPNQGLNQDCIFLG
                     KMLHFSEGGNDNHYETFTLAGSRLDSSGALATSLTGAVPTPVDVPYSSGGASAPVLTT
                     QQSLPQGLTAKVKINSSITNYSFGFVQSLGSLNAGVLQSGAQNTGLVYATTENSPNDS
                     ETAAAQKIGDNTPNTHLQSAQQASLCVTDGSRYAIITVGSNNAQLSVSVKQLGVVASC
                     PPSY"
     gene            complement(6379..7272)
                     /locus_tag="VN778_05500"
     CDS             complement(6379..7272)
                     /locus_tag="VN778_05500"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HXR50451.1"
                     /translation="MIAAVLVVLGLVFGSFVNALVWRIHEQAAETKKKNPNKKYLTQL
                     SIKKGRSLCPHCKHELAAKDLVPLLSWLSLRGKCRYCGKPISAQYPLVEAVTAVLFVG
                     SYRYWPHDLLFNHSAACPSPRLFHGVSWQADALFCLWLVLLTGLIALLVYDLRWFLLP
                     DRLIYPLSIVAALMALISIANASKPLVAFANLILAIAIGGGIFYLLFQLSGGKWIGGG
                     DVKLGWLLGLVVGTPGKALLFIFLASVLGTLISLPLLATHRLKRTSTIPFGPLLITGV
                     IIAQIFGAGILHWYQHNFLNF"
BASE COUNT         1794 a         1879 c         1905 g         1760 t
ORIGIN      
        1 gcctgctctt tcagccgagt cagctggcta cggccgtcgc gcgttgagaa attagcttgt
       61 tgttgggttt ggtaataatg catgttgcgg cgcagttcga acagatacga ttcgtaggat
      121 acccaggctg ggccggcttt ggctaccggg aacggcacca ccagcgacac atcatagata
      181 aagccggaag tcgattgcag tttgtagatc gcgagcaggc aaaacagcag gaatgcgact
      241 atgccaacca ctaaaatggt gacggaagtg cggacgatat tgtgcttgga atggcgcagc
      301 gggtagatgt acttacgcgc gccgccaagc acgtcttcac ggtgctcagc caccgtttcg
      361 ttggtgatac gcggcacact tgcgagcgct tcattgagct tttcttcggt cgctttgaca
      421 ctgcgcttga acgggcgctt gggcagctcc ggcaactgct tgggtagctt gggcttgggt
      481 aatttaggct tcttgggttt cttcatgcat ctcgtttgct aacttggagg agtcgccaga
      541 atacgggctg gcggcgttaa ttccctcgtc gcgcagaatt gataggagct gcttttggat
      601 cttagccggg tgatgcacat cgcgaatcac cagatctccc acatacgtct gcattttaat
      661 tgtaccaaac cccagcaggg tttcttgcag gcccgagatt tcataattta ccgattggat
      721 ttggctgagc cccagatcgg ctacagcgcg atggaacaaa cccttttgcg taatctgtat
      781 gaagcgctga tcggttacga taaagacgct aaagtgccag ctgatccagt ccggaaagaa
      841 tatcagcagg ccgagcaaac agccggcggc taggccacca aagaaaatgc cgaaaccgag
      901 gttggggttg atggctgctg gtataacgcc ggcgagcggt ccgagcatac ctaaaactag
      961 gcctttgcgc atcacgatcg gatgtttatg aaaaacaaag agaacttcct cgtcgtcgaa
     1021 ctggtcgtcg aaatatttgt ggcgtgtttg tgtggctgct gctttagctt ctttagccat
     1081 aagggtattg taccattcta tctggtatcc ccgaacggat tcgaaccgct atctgctcct
     1141 taggacggag ttattctatc cattgaacta cggggacatc accttaagtg tacgacgctt
     1201 cgctggcgtc tacaaaaagg ctcactgagc ctttttgggg cgcggctggg tgaatttttc
     1261 gtggacgctt tggtagtcgt ggatttcgct ctcggcaaac cagtgagcga tttccttgtc
     1321 ggcttcgtct ttttcggcgg aagcgtggat gatgttcccg actcccttgc caacgctggc
     1381 cgccgcgccg tagctgacat gggcaaaatc acccctgatg gtgcccggct gggcgctttt
     1441 cggctcggtc gggccgacca tcttgcggac ggtttcgaca gcatcgacgc cctccagcac
     1501 catggcgatc actggaccct ccatcatgct ggccagctgg cttttaaaaa tttcgtcgcc
     1561 tttacgggtt ttgagcgtac cgatgccctc gtaatgctca tagtaatgat cgtagtcggg
     1621 atccagcatc ttggtgccga cgattttgaa cccggctcgc tcgaagcggc taatgatttc
     1681 gccgacgatg ccgcgctgca cagcgtcagg cttcagaatg attaatgtgc gttccattag
     1741 caatcagtat accagctaca attattcagg tacatctttg attttgtaaa tatctggctc
     1801 ccagagcgca tcgtctagat tgacttttcg gccctcaatt tcggcttgcg cccaaaatcg
     1861 cacaaatgac tgtatagagt taaagcctag gtggagagcc ttgcgcttta aggcatcgcg
     1921 caattctctc tccatcggaa tctggacggt gaccattttg ggcgaatata gatacatgct
     1981 tacctccttg agtagcccta tataaaaaca ttatgtacgc agtatggttt catatgtttt
     2041 gataatgggt tggtactgat tttatatacc ctgctttatt attagttaac ggaaggctcg
     2101 ccgaaagcct ggcgcgcaag caacagaata tatctgaaac tccgcgataa gcaactgcaa
     2161 gcctggcaat tattaaatag tttcagatct tcgcttcact ccccatcaag gctccgggtg
     2221 gcgccacttg ccgccatgtc gtacaatacg attatgctat tcgcgaaagc caaaacggat
     2281 tttctggaat atcttgagat cgagcaaaac cgttcgcaaa agaccatcca aaattatgac
     2341 cattacctca cccggctcat cgacttcgcg ggtgacattc aggtaacaga cattgacgcc
     2401 gagctgatcc gcaagtggcg tttgtggctg aaccggcttg gcactaacac ctctgatgag
     2461 ctgggtaaaa cgactcagaa ttatcacctg atcgcgttga ggagctttct gaagttctgc
     2521 gccaagcgtg acgtccccgc tctcacggcc gataagatcg agctggccag gacgcagcgc
     2581 aaacaagtaa ctttcctcag cgaggacgag ttggcgcgtt tatttgctca gcctgatttg
     2641 aatacattaa gtggtctacg cgaccgagcc atactcgagc tactttttag ttcagggctg
     2701 cgcgtcagcg agcttgtcgg cctggaccga gaccatatca acctgaaacg gcgggaattt
     2761 atggtccgcg gcaagggcca aaaagaccgc ccgatcttca tcagcctgga agctgccaca
     2821 tggattgaac actatttggc caaacgcgaa gataaaacga agccactttt tatacgctac
     2881 agtggcacgc gcgccgttga tctcagcgga aactttcacc gcctgactgc ccgtagcatt
     2941 caacgcctag ttgcccgcta cgccctactg gccggcatca ccaaacatgt cagcccgcac
     3001 acgctgcggc attcctttgc cactgacctg ctgatgaatg gcgccgacct ccgctccgtc
     3061 caagccctgc tcggccacag caacatctcg accacgcaga tctatactca tgttacagac
     3121 ccgcacttac gagcggtgca tgagaagttt caccggcaca cggattaagt aaggccgagg
     3181 ctgtatcgtt attctaggtt gcaagcggtg ccgggacccg ccagcgaacc atcggcattg
     3241 agaaaattcg caccgttcgt tgggtccacc tggaaatgct tacagatatt gccagcctgg
     3301 agggcggcac tggggagcga caaggcagct tggctggaag actggtttgc cggcacccgt
     3361 acttggatcc gcttcaagac atttctggct tgacccgtga catcaatggt atcttgagcg
     3421 ccagagaagc taaatgacgc tccggtctga tctaccccat tgaagtagac ggatgcgtcg
     3481 tcataaaaat ccatgaagtg cagcaaatac atatcactgc tccctgcgac gtacggaata
     3541 ccataaatcg tcacgctgca tggcgccggc gtaaaagtag ttgagcagcg acctttaaca
     3601 atctgggcct gctgcgccaa atcataggca acacaagtag tgctgttggg ggcggccgga
     3661 caggcaggat tatttggacc agggctattt ttacttggat atgtataaat ggtaaacgtt
     3721 ttgtttatca aggcgttgcg atcaaaacta gtgctggaaa atggagtgat actgaactgt
     3781 aatactggcg gatggcccgg tgcggcattc caagcatttg taggagtgaa tgaaggagtt
     3841 tggcctggcg ggaaaaagct gtcgtgaggc agagccggcg tagcgtcacc gctgccccac
     3901 tggatcgtta gcgcaatagt gggtgtagca ggcgctgggc tcggcgtgaa aacactgctt
     3961 tgagagttac cgggtgctat attaaattct agatttggcg gcgttaaatc aaccattaca
     4021 caggtgtacg ccaccccgtg actagtatca atgtttcgat tcaggctaag tgaagtaagt
     4081 tggccgcctg tcaggcattt tttactggac gtcattggca aagaggcctc tgtaacgagt
     4141 ccttttttaa taccatccac aacgtcatta acgccgcttt cggcggcata gtatgcctga
     4201 ttggaaagct gcttgtcgag agcgttttgc tgctcccggc gagcaagttg ggcaaaaccg
     4261 atggtgagca gcgccaggac aatgatcaga atcaaagcca cgactataga agcgaatccc
     4321 tgctcattct cctgtatttt tttggtctct ggcatagaca taagcattgt actattaatt
     4381 actgcgttta tccactgtgg tcgtcagggt tgccgtagaa caaaattggt cgctcgtgtc
     4441 actagtacca aggcagacgc cagccggcga gagcgatggg tcaccgtacg caacacctac
     4501 attgatagtg tagggagttg tcgtggatga aagcgaaaag gcagtcagtc gagacttcgg
     4561 gccgatcagc tcggttccag aacccgcaac accaccattt gtggctgccg gatcagctgt
     4621 cgacaggtcg gccggagcac aactaccagc ggttgccagc gtgtcgcgcc atagcacgtg
     4681 gttaagcggc gcaccgatct gcataccaat aacgtacgaa tagcgcgttg tgtcgataca
     4741 gagagaatag attgattggc cgcttcttac taaagggctg cccgtggggt ttgctacggc
     4801 actcccaagc cgcaagtggt tagagacatc atcagtaata gtccggatat tatcctgtac
     4861 cctcgcctgg ctaacacctt tgtaaaacaa gttgccaatg ctaatcatga tggcagttgc
     4921 catgagcaaa attaccgaaa gaacggtggt agcaatcatc aactcgatga tggtgaagcc
     4981 agcttgctcc cgtacgctcc ttcgtctact catgggtgta ccttataaac ttgcgacacg
     5041 gtatcattac cccctcctat tacatcaggc caggtggcgg ttacgacaaa cgtatcatct
     5101 gataatatgc cgcagttagc atttctgaca agcgtgccgc aataaataac acttatcgat
     5161 gaactggacg aagcaaaagg cagtgcgcag ctcggatcgg gtggcgtcgg tgaaatatag
     5221 ggtttgacca cataggagcc tccattcagt acaacacaaa actggccagc aatcatgggg
     5281 gccgtacccg gtactgcgct aggcattgtc actctgagcc cttcaatctg gctctgcacc
     5341 agctcggaag ccacggcatt ctcctgcgct tggcgagcat ccaacaggct gcgattggct
     5401 gtggcgtagc atatgctaat cgcgaggccc agtaccgcca gaacaatcag cacttcgaca
     5461 atggtgtcgc ccttctccga ggtgcgtttg aactttaata gcttggcggg catgatgcca
     5521 ctactcctag ttgctttacg gacacgctta gttgagcatt attgctcccc acggtaataa
     5581 tggcatatcg agaaccatcg gttacacaaa gcgaggcctg ctgagccgat tgtaggtggg
     5641 tattcggggt gttatcgccg attttttggg ccgctgccgt ctcggaatca tttggtgaat
     5701 tttccgttgt tgcgtagacc aagccggtat tttgggcgcc gctctgcagc acgccagcat
     5761 tcaaactgcc caggctttgg acaaagccga aactatagtt tgtgatgctg ctatttatct
     5821 tcacttttgc tgtcaggcct tgagggagcg attgttgagt agttaaaacc ggcgcgcttg
     5881 ctccgccact actgtaggga acgtctaccg gggtcggtac ggcccctgtc aatgaggttg
     5941 ccagcgcgcc gctactatca agccgtgaac cggcaagcgt aaaagtttcg tagtgattat
     6001 cgttgccgcc ttcggagaaa tgcagcatct tacccaagaa tatgcagtct tgattcagcc
     6061 cttgattcgg cgaaccggcg gaagtaaagc tcagattgga gctgccgacc gcacaggtca
     6121 gtggaatgtc gctaaatcgt ccgtccacca cttgttcgat tgtgtcctgc aggcgagagg
     6181 ccatctcatt gactcctgcc gtgaatgcag tcttctcctg cttgccattg ataaaagtgg
     6241 ccgcaatcac gaacattaag ccagaaactg ccaagacaat catgacttcg ataatagtgt
     6301 accctagcgg ctgtttgttt ttgccaccca gcatcataat gtcctaagta tagcacaagc
     6361 gttatgaggt caaacagatt agaagttgag gaaattgtgt tgataccagt gtaggatgcc
     6421 agctccaaag atttgggcga ttattactcc agtgatcaag agggggccaa aggggatagt
     6481 gctcgtgcgc ttaaggcgat gggtggccaa aaggggcaaa ctgatcaggg ttccaagcac
     6541 cgaggccaga aagataaaaa gcaacgcctt tccgggcgtt cccacaacca agcccagtaa
     6601 ccagccaagc ttcacatccc cgccaccgat ccatttacca ccggataact gaaataacag
     6661 atagaatatg cctccaccga tggctatggc cagaatcaga ttagcaaagg ccacgagcgg
     6721 cttagaagca ttagcgatac taatgagggc catcagggcc gctacaatac tcaaggggta
     6781 gataagccgg tctggcagca aaaaccaccg caaatcgtac accaagagcg ctatgagacc
     6841 cgtcagtaac actaaccaca agcagaaaag tgcatctgcc tgccagctca cgccatgaaa
     6901 cagccgaggc gagggacagg ctgctgagtg gttgaatagc aaatcatgcg gccaataccg
     6961 ataagaacca acgaataaga ccgccgtaac cgcctcgact aagggatatt gggctgaaat
     7021 gggcttaccg cagtaacggc acttccctct taagctgagc cagctgagca gcggcactaa
     7081 atccttagcg gctagttcat gtttgcagtg gggacagagc gaccggccct ttttgatcga
     7141 tagctgcgtg aggtatttct tattagggtt tttcttcttg gtttctgcag cttgctcatg
     7201 tatccgccac actaaagcat tgacgaagct accgaacact agccccaaaa ctaccaaaac
     7261 agcagcaatc ataagcacta tactagcaca aaattaagaa caggctcttt cgagcctgtt
     7321 ctcgtgatgc ttacactc
//
LOCUS       DATNGP010000211         6898 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_10902, whole genome shotgun sequence.
ACCESSION   DATNGP010000211 DATNGP010000000
VERSION     DATNGP010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6898
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10902"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="VN778_05505"
     CDS             complement(<1..204)
                     /locus_tag="VN778_05505"
                     /inference="COORDINATES: protein motif:HMM:NF017911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50452.1"
                     /translation="MIVTLTGENSFSLHGELKQIVDSFVLEHGDLALERLDGEEVEFS
                     QIYEALTSLPFLSVKKLVILRTPS"
     gene            274..2451
                     /locus_tag="VN778_05510"
     CDS             274..2451
                     /locus_tag="VN778_05510"
                     /inference="COORDINATES: protein motif:HMM:NF011071.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming)/trehalose-phosphatase"
                     /protein_id="HXR50453.1"
                     /translation="MSQVIIVSNRLPVSVKRENGKLTFSPSIGGLATGLSSYIEDRKN
                     RWIGWPGIASDDLSERDKRKIIARLARHNCVPVFLTQKQIDDFYNGYSNSLLWPLFHD
                     LPFRDSNQAKHRQWWQAYRRVNNSFAEAVINIAGSNDRIWVHDYQLLLVPDMLRRQLP
                     RTNIGFFLHIPFPGPKTLSRLSEYKKLQLGVLGADLVGFHTSGYVSAFLENCSAARVG
                     TVGENQVLLNDRVTRVAEFPMGIDYEKYASANKSRVVKAAIRRFKRRYRGLKVIAAVD
                     RLDPSKGLVERLQAYRELLKREPKLRGKVIFSMIAAPSRTDIPAYQKLAKRLDELVAG
                     INKTYGRRGWQPVDYMNSAQPFEEVTALFAVADVAFIAPLRDGMNLAAKEFVASKRKR
                     GMLILSETAGAAEELQDALIVNPLRPESLVGALQQALTMPKRELRSRLKNMQHYLAGH
                     TVQNWAKTFVDTLQKPVPGTRPITRTLKGKLEQTLLKDYHQAEKRLLLLDYDGSLVPF
                     RENYTDAKPPQKLLNLLRSLSKRSDVVLVSGRQATDLEPWFKQLDIHLVAEHGALIKK
                     AGNKSWQTLVHGETKWKREVQPILEKYAALTPGARVEIKPHSLVWHYRASPSYYAQKY
                     AVTIRRILRPILKTYGVQIFQGNKILEVKDPRINKGAAAERWLKGSYDFVLAIGDDFT
                     DEELFQVLPFEVYSIKIGRGRSYARFRLGGSTEAQKLLKKLAR"
     gene            complement(2440..3321)
                     /gene="mutM"
                     /locus_tag="VN778_05515"
     CDS             complement(2440..3321)
                     /gene="mutM"
                     /locus_tag="VN778_05515"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF002211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HXR50454.1"
                     /translation="MTMPELPEVETVRIGLASLLPGREIATVDFDWPKSFPNASMDVE
                     QFIIGAKVVTVARRAKVLLIELSSKYSLVIHLKMTGQLVFRGLEAHFGAGHPNASLVG
                     DLPDKSTRVTLTFTDGSRLFFNDQRKFGWMRLIPTAEVVHLDFFQKVGPEPLSTDFTW
                     QEMRERLLRRQNSNVKAALLDQTIIAGVGNIYADESLWGAKIHPSALVKNLSARQFHK
                     LYDELIYVLKLAIKKGGSTDKNYVNAEGKSGSYLSFARVFRREGKPCPRCGTTIIKTR
                     VAGRGTHLCPHCQKLSS"
     gene            3347..3586
                     /locus_tag="VN778_05520"
     CDS             3347..3586
                     /locus_tag="VN778_05520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50455.1"
                     /translation="MDSLSSILGNKDFDEPPEMSSIKKYVQDEFHTAVSVQVRERDIV
                     IQVPSAALANTLRLRSPDIKRRCQIDKRLTFRIGQ"
     gene            complement(3563..4144)
                     /locus_tag="VN778_05525"
     CDS             complement(3563..4144)
                     /locus_tag="VN778_05525"
                     /inference="COORDINATES: protein motif:HMM:TIGR01509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HXR50456.1"
                     /translation="MIKAVIFDCFGVLTTDNWQKFLSSLPEEADLESAREAHRAYDKG
                     LLSKQEAAEQIQAATGKSFTEIDDASNDEIVKNTPLLEYVRELHQEGYKTSILSNVAS
                     NWIREQLLTPDEQAYFDDFLLSHEVGLTKPDPRMFKLAAERLDIELQKAVLVDDKESY
                     CTAARELGMQVVWYQDFKQMKAELKQLLADAKR"
     gene            complement(4172..4735)
                     /locus_tag="VN778_05530"
     CDS             complement(4172..4735)
                     /locus_tag="VN778_05530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50457.1"
                     /translation="MRYFIGFLITIGLIILLIVLLFGGGGNNVPKTPAHQPLVSYANT
                     DAETRLTIDGPINAQQSHDQVQITVGRGQITYDQIKGYDGHVVNQQTYSNTENAYNNF
                     LHALVVAGFTHGNTSTTLKDELGYCPLGNRYIFELFEGSNYIERYWSTNCGNPKTYLG
                     STPLTLTLFQNQVPNYADLVQNLSLQQ"
     gene            4838..5287
                     /locus_tag="VN778_05535"
     CDS             4838..5287
                     /locus_tag="VN778_05535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50458.1"
                     /translation="MRFKYETGIITFVQFIVLSLLAIANGLNSVVTTCHDSSPDCISN
                     LLVSLIFFLLTVAWFAAIWVVGFAAQERRSRRLAQLLIAAEALIALIALFNAKHHTDW
                     LSLFTSLTDLVLAIWVITLAWRLMRAKGGRVVTRQRPRQRRRHKADL"
     gene            complement(5282..5899)
                     /locus_tag="VN778_05540"
     CDS             complement(5282..5899)
                     /locus_tag="VN778_05540"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXR50459.1"
                     /translation="MKNLRQQTVDVYNDKAQDMAEKFRAIPPRVKDIELALRLAGNPK
                     KPRILEIGCGDGRDAKEIFARAGWFLGIDISEELIKLARLHEPRASFEVADAVEFDYP
                     HNLDAVFAFASFLHLNKAEVKIIFDKLTQAMKSGGVIYLSLKYAPHYKSEVNTDKFGT
                     RQYYFYNPELIKKLAGKNFVTLKTWREVRPGANHADWVEVILQKL"
     gene            5975..6151
                     /locus_tag="VN778_05545"
     CDS             5975..6151
                     /locus_tag="VN778_05545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50460.1"
                     /translation="MSETRVRVADTGTFTPREWLRREVLWASLMIPYTEQYAGWFNEK
                     ELYHHKGNSSRQAL"
     gene            6172..6663
                     /locus_tag="VN778_05550"
     CDS             6172..6663
                     /locus_tag="VN778_05550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50461.1"
                     /translation="MQAKAFFNHGKVIGGLVSSVAVFRMRMNGPLDPNNEISEVVLQM
                     QAKSIDKALELPLLQATNGTKGEQEVFEECTHLAKLAVRNPGNQFVNRRYMLRAAKSM
                     SSLACQNVVLWLETSKQDEAESLPLLTIPDGGTPRSAGPRVEMLQPIFHQIIASEAFT
                     RSS"
     gene            complement(6666..>6898)
                     /locus_tag="VN778_05555"
     CDS             complement(6666..>6898)
                     /locus_tag="VN778_05555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50462.1"
                     /translation="IELADLLEVIEQIGKELGKDFSTLRKLQQERHKKRGGFDKRIFV
                     ERLDLQEDDPWADYYAGEPDRFTEVISKKAKR"
BASE COUNT         1673 a         1843 c         1652 g         1730 t
ORIGIN      
        1 gctcggggtg cgcaggataa ccagtttctt gacagacaga aacggcaagc tggtaagcgc
       61 ttcgtaaatt tgtgaaaact ccacttcttc cccgtccagg cgctctaggg ccagatcacc
      121 atgttcaagc acaaagctgt ccacgatttg cttaagctcg ccgtgcagcg agaagctgtt
      181 ttctcccgtc agcgtcacga tcatgcagtc attgtaacaa ttgacactct gcaagcttaa
      241 cacttctgct atgctacaag ggttgcacaa attatgtcgc aggttattat tgtttccaat
      301 cgtcttcctg tcagcgtcaa aagagaaaac ggcaagctga cattttcgcc cagcatcggc
      361 ggcttggcga ctggcctgtc atcttatatc gaagaccgga aaaatcgctg gatcggctgg
      421 cccggcattg ccagcgacga tctgtcggag cgggataaac gcaaaattat tgcccggctt
      481 gccaggcaca actgcgtgcc tgtttttttg actcaaaaac agatcgatga cttttataac
      541 ggctatagca attcgctgct ctggccgctc tttcatgact tgcccttccg agatagcaac
      601 caggcgaagc accggcaatg gtggcaggcc tatcggcgcg tcaacaacag ctttgccgaa
      661 gccgtgatca atattgctgg aagcaacgat cgtatctggg tccatgacta tcagctgttg
      721 ctcgtgccgg atatgttgcg cagacaactg ccgagaacca atattggctt tttcctacac
      781 ataccgtttc ctggcccgaa aactctttcc cgcttgagcg aatacaaaaa actgcagctc
      841 ggtgtactcg gcgctgatct cgttggtttt catacttcag gttatgttag cgcttttcta
      901 gaaaactgtt cggcggcgcg tgtcggcact gtcggcgaga atcaagtcct cttgaatgat
      961 cgcgtgacgc gggtggcgga attcccgatg ggcattgact acgaaaaata tgcgtcggcc
     1021 aacaaatcca gagtggtcaa agccgccatc cgccgcttca agcggcggta ccggggactg
     1081 aaagtcattg ctgccgttga tcgcttagat cccagcaaag ggctcgtcga gcgcttgcaa
     1141 gcctaccgcg agcttttgaa gcgcgagccg aaacttcgcg gcaaagtcat attttcaatg
     1201 atcgccgcgc cttctcggac cgatatccca gcctatcaga agctagctaa gcgcctcgac
     1261 gaattggtag ctgggatcaa caagacgtac ggccgccgcg gctggcagcc agtcgactac
     1321 atgaacagtg cgcagccttt tgaggaggtc acggcattgt tcgcggtcgc cgacgtggct
     1381 ttcatcgctc cgctgaggga cggtatgaac ctggccgcca aagaatttgt cgccagtaag
     1441 cgcaaacgcg gcatgctgat tttgagtgaa accgccggcg ctgccgaaga gctgcaagac
     1501 gccctgatcg ttaatccact ccgtcccgaa tccttggtcg gcgctctcca gcaagccctg
     1561 actatgccca agcgtgaact gcgttctcgc ctcaagaaca tgcagcacta tctcgccggc
     1621 cataccgttc agaattgggc caaaactttc gtcgatactt tgcaaaagcc cgtgcctggc
     1681 acccggccga tcaccagaac gctcaaaggc aagctagagc agacgctcct gaaagattat
     1741 caccaagccg agaaacgact ccttctactg gattatgacg gtagcctagt gccattccgc
     1801 gaaaactaca ctgacgccaa accgccgcaa aaactactta atttacttcg atcgctcagc
     1861 aaacgtagtg atgttgtgct agtcagtggt cgccaagcta cggatctgga gccttggttt
     1921 aagcaactcg acattcactt agtcgcagag catggtgcac ttatcaaaaa agccggtaat
     1981 aagtcctggc aaacgttagt ccatggagaa accaagtgga aacgagaggt ccagccaatt
     2041 ctggagaaat atgccgccct cacccctgga gcgcgcgtgg aaatcaagcc ccactctctc
     2101 gtctggcact accgggcttc accttcctat tacgcgcaaa aatatgccgt cactatcagg
     2161 cgcatcctgc ggcctatact gaaaacctat ggcgtgcaaa tctttcaagg caacaagatt
     2221 ctggaggtca aagatccgcg catcaacaaa ggtgcggccg ctgaacgctg gcttaaaggc
     2281 agctatgact tcgttttagc aatcggtgac gacttcaccg acgaagaact tttccaagta
     2341 ttaccctttg aagtctacag catcaaaatc ggccggggcc gctcctacgc ccgcttccgc
     2401 ctgggcggct ccactgaagc ccaaaagctt ttaaaaaagc tagctcgata gcttttggca
     2461 gtgtgggcac agatgcgtac cgcgcccagc gacgcgggtt ttgatgatgg tggtgccgca
     2521 gcgcgggcaa ggcttgcctt cccggcgaaa gacgcgggcg aagctgaggt agctgccgct
     2581 tttaccttcg gcattcacgt aattcttatc tgttgagccg ccctttttga tggctaattt
     2641 cagtacgtag atcagttcgt cgtacaactt atgaaactgc cgcgcggaaa ggtttttaac
     2701 tagtgcagac ggatggattt tagcgcccca cagcgattcg tccgcataaa tattgccgac
     2761 accagcgata atcgtctgat ctaaaagcgc ggctttaacg tttgagttct gccgccgcag
     2821 caggcgctcg cgcatctctt gccaggtaaa atcagtgctc agcggttccg gcccaacctt
     2881 ctggaaaaag tccaaatgaa cgacttcggc tgtggggatc aggcgcatcc aaccgaattt
     2941 gcgttggtca ttaaagaaca gccggctacc atcagtaaat gttagcgtga cgcgcgtcga
     3001 tttatccggc aaatctccaa ctagtgaagc atttgggtgg cccgctccga agtgcgcttc
     3061 caagccgcga aagactagct gcccggtcat tttcagatgg atcaccaacg agtatttact
     3121 gctgagctca atgagtaaaa ccttcgctcg gcgcgctacc gtgacaacct tggccccgat
     3181 aataaattgc tcaacatcca tcgacgcgtt cggaaacgac ttcggccaat cgaaatccac
     3241 ggtcgcgatt tcccgccccg gcagcaagct cgccaggcct attcgaacag tttcaacttc
     3301 aggtaactcc ggcatcgtca tcttatttta caagtataat aagggcatgg atagcttgag
     3361 cagcatactg ggcaataaag atttcgatga gccgccagag atgtcgagca tcaaaaaata
     3421 tgtgcaggac gaatttcaca ccgcggtgtc ggtgcaagtg cgcgaacgcg acattgtgat
     3481 tcaagtgccc agtgcggcgc tcgccaacac cttacggctg cgaagtcccg acatcaagcg
     3541 ccgttgtcag atcgataagc gcctaacgtt tcgcatcggc cagtaactgc ttaagctcgg
     3601 ctttcatctg tttaaaatct tgataccaca ccacttgcat gcccagctcg cgggcagccg
     3661 tgcagtagct ttccttgtca tcgactagca ccgctttttg cagctcgatg tcgagccgct
     3721 cggctgccag tttaaacatg cgaggatcgg gcttggtcag gccaacttcg tgcgacaaga
     3781 ggaaatcatc gaaatatgct tgctcatcgg gagttaggag ctgctcgcga atccagttac
     3841 tggcaacatt actgagaatg ctagtcttat agccttcttg gtgtagttca cggacatatt
     3901 cgagcaacgg cgtatttttt actatctcat cattactagc atcatctatc tctgtgaagc
     3961 tcttcccggt cgccgcttgg atttgttcag cggcctcctg tttgctaagc aatcccttat
     4021 cataagcccg gtgtgcttca cgcgcacttt caagatcggc ttcctctggc aggctactta
     4081 aaaacttctg ccaattgtcc gttgtcagca ccccaaaaca gtcaaagata acagctttga
     4141 tcatacttta attatacgac ggggctgacg actattgctg taagctaaga ttctgtacca
     4201 aatcggcgta attcggcact tggttttgga agagcgtcag cgtcagaggc gtacttccga
     4261 gatatgtctt ggggttgccg caattggttg accagtagcg ttcgatataa ttacttccct
     4321 caaaaagctc aaagatgtag cggttgccaa gcggacaata accgagttca tccttcaacg
     4381 tagtgctcgt attaccgtgg gtgaagccgg ccaccacgag ggcatgcaag aaattgttat
     4441 aggcattctc ggtattcgaa taggtttgct ggttcacgac gtggccgtcg taacccttga
     4501 tctgatcgta ggtgatttgg ccgcgaccga cggtgatctg tacttggtcg tggctttgct
     4561 gggcattaat tgggccgtcg atggttaagc gggtttcggc atcggtgtta gcatagctaa
     4621 ctaagggttg gtgggccgga gttttcggca cgttattgcc accaccgccg aacaagagca
     4681 caatcaaaag aatgatgagc ccaatggtga tcagaaaacc tataaaataa cgcatgtttc
     4741 catctctcag cttatttagc taatatatct taacacgata acgcttattt tgcaacccct
     4801 taccggtgct taactgtttg tgctttaata agtgtgtatg cgtttcaagt acgaaaccgg
     4861 catcattact ttcgtccagt tcatagtgct cagtttgctc gctatcgcca acggtcttaa
     4921 ttccgtcgtt accacctgtc acgactcctc acccgactgt atcagcaatc tgttagtctc
     4981 gctcatcttc ttcttgctca cggtggcctg gtttgccgct atctgggtag tcggcttcgc
     5041 cgcccaggaa cgccgcagcc gtcgcttagc ccaactccta atcgccgccg aagccttaat
     5101 cgccctcatc gccctcttta acgccaaaca tcacactgac tggctgagcc tgttcaccag
     5161 cctcaccgac ctcgtcctcg ccatctgggt catcacccta gcctggcgcc tgatgcgcgc
     5221 caaaggcggc cgcgtcgtca cccgccagcg cccccgtcaa cgccgccgcc ataaagcgga
     5281 tttataattt ctgtaaaatt acctcgaccc aatctgcatg gttagcgccc ggcctgactt
     5341 cccgccacgt ttttagagta acaaagttct ttccggctaa ctttttgatg agttccggat
     5401 tgtagaaata atattgccgg gtgccaaatt tatctgtatt aacttctgat ttgtaatgcg
     5461 gcgcgtattt aagcgacaag taaattactc ctcctgactt cattgcctgt gtcagtttat
     5521 cgaaaataat tttaacctct gccttgttta ggtgaagaaa agaagcaaag gcaaataccg
     5581 catctagatt gtgtggatag tcaaattcca cggcatcagc aacctcaaag ctggcccgcg
     5641 gctcatgaag tcgagccagc ttaataagtt cttcggaaat atcgataccg agaaaccatc
     5701 cagctctggc aaatatttct ttagcatcgc gcccatcgcc gcagccgatt tccagtattc
     5761 tgggcttttt cggattaccc gccaatcgca gagcgagttc tatatcttta acgcgtggtg
     5821 ggattgctct gaatttttca gccatgtcct gagctttgtc gttataaaca tccactgttt
     5881 gctggcggag attcttcatt gactgctatt ttaacagatg atgatgctgg gctacctctt
     5941 tacttttgcg ccaaaagtgt tattgtaaag cattatgtct gagacacgag tacgagtagc
     6001 ggatacgggc acgttcactc ctcgcgaatg gctgcgccgt gaagtattgt gggcttcact
     6061 gatgataccg tacaccgaac agtacgcggg ctggtttaat gaaaaggagc tgtatcatca
     6121 taagggtaat agctcccggc aagcgttata agcagtggtc aagatagcga aatgcaggcc
     6181 aaagcttttt tcaatcatgg caaagtcatc ggtggtctag tatcatcagt tgctgtcttc
     6241 agaatgcgca tgaacggacc gcttgatcca aacaatgaaa tttcggaagt cgttttacag
     6301 atgcaagcca agagcattga taaggcactg gagcttccac tattacaagc tacgaatggc
     6361 acaaagggtg aacaagaagt ttttgaagaa tgcactcacc tggcgaagtt agctgtccgt
     6421 aatcccggga accaatttgt gaaccgtcgt tacatgctca gagcagcaaa atccatgtcg
     6481 tctttagctt gccaaaatgt ggtgctttgg cttgaaacaa gcaagcagga tgaggcagaa
     6541 tccttacccc tgctcaccat tccagacgga ggcacgccaa ggtctgcagg tcctcgcgtc
     6601 gaaatgctac agcctatatt ccatcaaatt attgcttcag aggcttttac acgatcgagt
     6661 tgaaatcaac gctttgcttt cttcgaaata acttcagtga atcgatctgg ttcaccagca
     6721 taataatcag cccacggatc atcctcctgc aaatctaacc gctcgacgaa gatccttttg
     6781 tcgaagcccc ctcgcttctt gtgcctttct tgctgaagct tccgaagcgt actgaaatct
     6841 ttgcccagtt ctttgccaat ctgctcaatc acctcgagta agtcagccaa ctcgatgt
//
LOCUS       DATNGP010000212         3042 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_1492920, whole genome shotgun sequence.
ACCESSION   DATNGP010000212 DATNGP010000000
VERSION     DATNGP010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3042
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1492920"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..479)
                     /locus_tag="VN778_05560"
     CDS             complement(<1..479)
                     /locus_tag="VN778_05560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50463.1"
                     /translation="MKAEHLSEQLAGAAYTHSLIDTEELMKPEVSRGDFLLAVTGEAI
                     VQTIPGADLESDSVDGERYESFSLPGDVIKKVHDKKTHEILLESEITPADYERQAEAL
                     GPEHVRAARLAKHLTNRLKDALDGRAIEEVSSTGKGGGVQMILMALGNYMQKSGVDW"
     gene            644..1300
                     /locus_tag="VN778_05565"
     CDS             644..1300
                     /locus_tag="VN778_05565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50464.1"
                     /translation="MKKHYHFTLKACLVMVIAYIKLGVFVSKTNQEAEHTIVEQRIMD
                     IRLPARYDQDSPRICLLQIQKAETFSEETEAKRKALEEALYCDYEDYDESELGDRPAG
                     TDLKADVRVCGIGGCTLRIFQAEDGSDIQTGECKAAGYCLREKFKAVGKAATVQEGRA
                     LYREIIDICDGAVSSMSAGNFCPKLDCDLSAGVSEDQIPGTDGTCTIKNLVQLEGFEQ
                     "
     gene            complement(1301..1375)
                     /locus_tag="VN778_05570"
     tRNA            complement(1301..1375)
                     /locus_tag="VN778_05570"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1340..1342),aa:Gly,seq:ccc)
     gene            complement(1382..1458)
                     /locus_tag="VN778_05575"
     tRNA            complement(1382..1458)
                     /locus_tag="VN778_05575"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1422..1424),aa:Thr,seq:cgt)
     gene            1558..1869
                     /locus_tag="VN778_05580"
     CDS             1558..1869
                     /locus_tag="VN778_05580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50465.1"
                     /translation="MEFEERGPARKAETLNDFTRIAAAMIEASARITDGASYQRAIVS
                     VRESTGYRAIDISTMVMLLIDEEVLEHTTNINRRGSALTPLGQTIHQNMCDQLGLDGP
                     S"
     gene            complement(1864..2301)
                     /locus_tag="VN778_05585"
     CDS             complement(1864..2301)
                     /locus_tag="VN778_05585"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HXR50466.1"
                     /translation="MKASVTVKITVEASCEEVFRYFKHLKYHQLWNPHLLKISPLTTI
                     KAGLVYQTTNLLLGVKVKSQNHVLKVVPNQEFEVENTTGPLNYWVKYRLQERPEGTLV
                     TCTSTVTAHGETFAFTKPVLRLLVQRELRSDLQALKLAAEHRL"
     gene            complement(2338..>3042)
                     /locus_tag="VN778_05590"
     CDS             complement(2338..>3042)
                     /locus_tag="VN778_05590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50467.1"
                     /translation="ATTLALGARATTASTSAFCTNLPTKASDITAKLNDLTGKVTQAW
                     SQQDQKLTADEAKVDQTVTADRARADAERAADFAKLEAKATTDSEKQAVTTYEATVKD
                     AVSTRRAAYDAARQTFRDGVKSAITARQSTITGQMSTFQSSASSALATAEASCASNAT
                     NGATIRQTLVASLKSARQTFESQRQADATVGNQVSQLAATRNTAFQTADQTFKNSMTT
                     ARTALKQAFGNKSSTV"
BASE COUNT          711 a          745 c          798 g          788 t
ORIGIN      
        1 tgccaatcca cgcccgactt ctgcatgtaa ttacccaaag ccattaatat catttggact
       61 ccgccgccct taccggtgga gctgacttcc tctatcgccc gcccgtccag cgcgtctttc
      121 agccgattag ttaggtgctt agctaacctt gcagcccgga catgctcagg accaagcgct
      181 tccgcttgcc gttcgtaatc ggccggtgta atctcgcttt ccagcagtat ttcatgggtt
      241 ttcttatcgt gaactttttt tattacatca ccgggcaatg aaaatgattc gtaccgttca
      301 ccatcgacag aatccgactc aagatcggca ccagggatcg tttgcacaat agcttcgcca
      361 gtgacagcga gcaggaagtc cccgcgggat acttccggct tcattagctc ttctgtgtcg
      421 ataagggaat gagtataagc tgcaccggcg agttgctcgc taaggtgttc tgctttcatt
      481 gctttatgtg tattaccttc gttaataagt gttaagcata agtatccacc tggagcttac
      541 gcatgtcaat aagtaagtaa gatttcttac aatggcgcag cacagtgtcg aaagttatta
      601 ccattcttcg tagctataaa gtaactgttt gagttaagtc ggggtgaaaa aacattacca
      661 ttttacttta aaagcttgct tagttatggt tattgcatat attaaattag gagtgtttgt
      721 gagcaaaact aatcaagagg ctgagcatac aattgttgag caacgcatca tggacattag
      781 attacctgca cggtatgatc aggactcacc tcgcatctgc ttgctacaaa ttcaaaaggc
      841 cgaaacattc agcgaagaaa ccgaagctaa gcggaaagca cttgaggaag ccctgtattg
      901 tgattatgaa gattatgatg aatcagagct gggtgacaga cctgcgggaa ctgatcttaa
      961 agctgatgtg cgcgtttgtg gtatcggagg ctgtactctc cgtatctttc aagcggaaga
     1021 cggtagtgac atccagactg gtgaatgcaa agcagctggc tattgtttaa gggaaaaatt
     1081 caaggcggtt ggcaaagcgg cgacagttca ggagggccga gcgctgtatc gagaaattat
     1141 tgatatttgc gatggcgcgg taagctcaat gtcagccggt aatttttgcc ctaaacttga
     1201 ttgtgactta agcgcaggtg tgagtgagga ccagattcca ggtacagatg gtacttgtac
     1261 gatcaaaaac ttagtccaac tcgaaggttt tgagcagtaa tggtgcgggt ggcgggagtc
     1321 gaacccgcgt cttagccttg ggaaggctac ataatagccg ttatacgaca cccgcgaagt
     1381 ctggagccac ctacgagatt cgaactcgtg acctccgctt tacgaaagcg gcgctctagc
     1441 caactgagct aaggtggcga aaatctatag tattataaca gcctagtatc aggtctgttg
     1501 catcgggcgt aatgtattcc acagccgagt aggcacaaac tatctatact ttctgcgatg
     1561 gagttcgaag agcgcggtcc agcccgcaaa gccgagacat taaatgattt tacccggatc
     1621 gccgcagcca tgattgaagc ttcggctagg attacggacg gcgcatcata ccagagggcg
     1681 atagtcagcg tgcgcgaatc gaccggttat cgagctatcg acatttcaac catggtgatg
     1741 ctgctcatcg acgaggaagt actggagcac accacgaata tcaatcgccg aggttcggca
     1801 ttaacgccgc tcggccaaac cattcatcaa aatatgtgcg atcagctcgg cctcgatggc
     1861 ccctcataaa cggtgctcag ctgccagttt gagcgcctgc agatcggagc gcagttcgcg
     1921 ctggaccagc agtcgcagta caggcttggt gaaagcgaac gtctcgccgt gagccgtgac
     1981 agtgctcgtg caagtaacaa gcgtcccttc tggcctctcc tgcagccggt atttaaccca
     2041 gtagttcaga ggaccggtgg tgttttctac ttcgaactct tggtttggaa cgacttttaa
     2101 cacgtgattt tgagacttca ccttaacgcc caaaagtaag ttcgtcgttt gatacaccaa
     2161 gccggcttta atagtcgtca gcggcgatat tttcagtaga tggggattcc agagttggtg
     2221 gtacttgagg tgcttaaaat accgaaacac ttcttcgcag ctggcttcaa cggttatctt
     2281 gacggtaacg ctggctttca tagcttcgtc gatatgtcta ccggcgcaaa cggcagctta
     2341 gacagtactg cttttattgc caaaggcttg cttgagtgcc gtccgggcag tagtcatgct
     2401 gtttttaaac gtctggtcgg cagtctgaaa ggcagtgttt cgggtagctg ccagctgact
     2461 gacttgattg ccgacagtgg catcagcttg gcgttggctt tcgaatgtct ggcgagccga
     2521 cttcaggctg gctaccagcg tttggcgaat ggtggcgcca ttggtagcat tgctggcaca
     2581 actcgcttcc gctgtcgcaa gcgctgaact ggccgagctc tggaaagtgc tcatttgacc
     2641 agtaatcgtt gattggcggg cagtgatagc actttttacg ccgtcacgga aagtctggcg
     2701 agccgcatca taggcggcgc ggcgcgtact gaccgcgtct ttcacggtgg cttcgtacgt
     2761 ggtgacagct tgcttctccg agtcagtcgt ggctttggct tccagcttgg caaagtccgc
     2821 ggctcgctct gcatcagccc gcgcccgatc cgccgtcacc gtttgatcga ccttagcctc
     2881 atcagccgtc aatttttggt cctgctgcga ccaagcttgc gttaccttcc cggtaagatc
     2941 attcagcttc gccgtgatgt cacttgcttt ggttggcaaa ttcgtacaga aggcgctggt
     3001 cgacgccgtg gttgcccgcg cgccaagcgc caaggtagtc gc
//
LOCUS       DATNGP010000213         4097 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_2844188, whole genome shotgun sequence.
ACCESSION   DATNGP010000213 DATNGP010000000
VERSION     DATNGP010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4097
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2844188"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(23..1546)
                     /locus_tag="VN778_05595"
     CDS             complement(23..1546)
                     /locus_tag="VN778_05595"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010165613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HXR50468.1"
                     /translation="MPTTNEKIGHLIAQIRQDKGLTQAEFARKLKTSQSAVNRIEHGR
                     QNLSMETLGRISDVLNKQLISINQGAINLYIEGGHELKGEITLKTSKNATVALLCASL
                     LNKGTTRLKNVARIEEVNRLIEVLASIGVQVRWTNGNDLEIKPPARLDLANMDKEAAR
                     KTRSVLMFIGPLMHHAKEFDIPYAGGCELGRRTVLPHLYGLEEFGVGVLTKSGHYRVM
                     ATPMLPVRSVVLYESGDTTTENILMAAALTPGETVIKMASANYMVQDLCFFLQKLGVH
                     IEGIGSSTLRVHGVDKIHKNVTYAPSEDPVEAMTFIAAAVATDSTITIKRAPIEFLEL
                     ELLKLEKMGLKYEISGSYLADNGHTELVDLKIHKHNGELLAPKEKIHPNVFPGLNIDH
                     LPYFVPIAAVAKGRTLIHDWIYEDRALMYTEMKKIGVSLELADPHRVFIQGPTRWKPA
                     DLVCPSGIRPAVMLLIGMLAAPGNSILRNVYTINRGYEDLAERLNSLGAHITVLHEI"
     gene            1736..3034
                     /gene="glyA"
                     /locus_tag="VN778_05600"
     CDS             1736..3034
                     /gene="glyA"
                     /locus_tag="VN778_05600"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HXR50469.1"
                     /translation="MSEEERAMIDELIAKLIAAEEKRQREGLELIPSENYVSRDVLTA
                     MGSVFTNKYSEGYPGKRYYGGQDYTDQVEQLAIDRAKKLFKADHANVQPHSGAPANEA
                     VYSAWLEPGDTVLAMDLSHGGHLTHGAPVTRSAHLYNFIRYKMKDPATGEIDYDELRE
                     LARKHKPKIVLAGFSAYPRELDYAKFAEIGNEVDALLMADMAHIAGLIVAGVAKNPFD
                     YGFHVITTTTHKTLRGPRGGLILSQGAVGNPFKAPEKTLENLPTLIDRSVFPGMQGGP
                     HEHIIAAKAVAFGEALKPKFKTYATQIVKNARALADALQARGFYLVTGGTSNHLILSD
                     VHKSFGIDGDVVQEVLDSVGLTLNKNAIADDPLPPSKPSGIRLGTPAITTRGLVETDM
                     EKIAQWMKQAIDNRENKTTLNKLRNDVKQFALQFPLPSDK"
     gene            complement(3036..3665)
                     /locus_tag="VN778_05605"
     CDS             complement(3036..3665)
                     /locus_tag="VN778_05605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50470.1"
                     /translation="MSAENQGDRLKEFTGLALFHATSTFDTWCFNLCAAHSYLDTDPA
                     HYETTISHAQTRTCESFNELANVIAADHEIGNLEDRLTALLLIKVGFEQGRLTKLKKG
                     LLADVADNTAVVEIEVQEYMQIAHEFPTDRDLCTIIKEDFAERLKEDIASLQWTKPQP
                     TPKPDLPLSQPDKPPLSRQNLLGLAVLSLLGAGALATLYSMRMGQHKAH"
     gene            complement(3729..>4097)
                     /locus_tag="VN778_05610"
     CDS             complement(3729..>4097)
                     /locus_tag="VN778_05610"
                     /inference="COORDINATES: protein motif:HMM:NF014872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50471.1"
                     /translation="ISIRSAKLPDPALVHNVGSFFANPIVTEEQYSYLADNYDSIPHW
                     SVGTGSVKLSAAWLIEQAGLKDIHDQETGMATWPRQPLVLVNEHARSTADLLKFKQKI
                     IDIVQQKFNITLVQEPELLP"
BASE COUNT          962 a          996 c         1130 g         1009 t
ORIGIN      
        1 gggcatatat ttaagaaaac ggttagattt cgtgcaggac ggtgatgtgg gcgccgaggc
       61 tgttgagccg ttcggctagg tcttcgtagc cgcggttaat agtgtagaca ttcctgagga
      121 tcgagttacc aggggcggcc agcataccga tgaggagcat cacggccggc cgaataccac
      181 tggggcagac caggtcggcg ggcttccagc gggtcggtcc ttggatgaaa acacggtgcg
      241 gatcggccag ctcaaggctg acgccgatct ttttcatctc ggtatacatg agtgcccggt
      301 cttcgtaaat ccagtcatga atgagcgtcc ggcctttcgc aacggcagcg atggggacaa
      361 aatacggcaa atggtcgatg ttcaggccgg ggaagacatt cggatggatc ttttcctttg
      421 gtgccagtag ttcgccgtta tgtttgtgga tttttaagtc cactagctca gtgtggccat
      481 tgtcagccag gtagctgccg ctgatttcat acttcaggcc catcttttcc agcttgagaa
      541 gttctaactc caagaattcg attggcgcgc gcttgatagt tattgtcgaa tcagtagcga
      601 cggcagcggc aatgaatgtc atggcctcga cgggatcttc gctcggtgca taagtgacgt
      661 ttttgtggat cttgtctacg ccgtgcacgc gcaaagtgct cgagccaata ccctcaatgt
      721 ggacgcctaa cttctgcagg aagaaacaca agtcctgcac catatagttc gcactggcca
      781 tcttgatgac agtttcgccc ggcgtcagcg ccgccgccat caaaatgttt tctgtcgtcg
      841 tgtcgccgga ttcatataat acgactgatc tcacgggtaa cattggcgtg gccataaccc
      901 ggtaatgacc ggacttagtc agtacgccaa cgccaaactc ctccaagccg tacaaatgcg
      961 gcagcacagt gcgccggcca agctcgcagc cgccggcgta aggaatatcg aactccttgg
     1021 catgatgcat aagcggcccg atgaacatca gcacactgcg agttttgcgg gctgcctctt
     1081 tgtccatatt ggctaggtca agacgagccg gcggtttgat ctctaggtcg ttgccattcg
     1141 tccagcgaac ctgtacaccg atactggcta agacttcgat gagtcgattg acttcttcga
     1201 tccgggcgac atttttcagg cgggtggtgc ctttattgag tagactggcg cagagcaggg
     1261 cgacagtcgc atttttgctg gttttaaggg tgatttcacc ctttagttcg tggccgcctt
     1321 cgatgtagag attaatcgcg ccttggttga tgctgatcaa ctgcttgtta agcacatcgc
     1381 tgatacgtcc gagcgtttcc atgctcaagt tttggcggcc gtgttcgatg cggttgacag
     1441 ctgattggga ggtttttaat tttcgagcaa attctgcttg ggtgaggccc ttatcttgcc
     1501 ttatttgagc aataaggtgg cctatcttct catttgtcgt gggcatatgc tcaaaatgtt
     1561 accatatatg gtattttatt gcaacctagg tttttgtttg gaaagcttgc tataatcata
     1621 gcatgcttta gcttactctc cattactgac ttatattcga ggagcggagc aactcaaaac
     1681 ttgttttgat gttgcgagta ccgaaggata tatcacttgc gatatactca atgtaatatc
     1741 cgaagaggag agagccatga ttgacgaact aatagccaaa ctgattgccg cagaagaaaa
     1801 acggcagcgg gaaggcttag aattaattcc ctcagaaaac tatgtcagcc gtgatgtatt
     1861 aacggccatg ggcagcgttt tcacgaacaa gtattcagag ggttatccag gtaagcgtta
     1921 ctatggcggc caggactaca ccgatcaagt ggagcagctg gccatcgacc gcgccaaaaa
     1981 gctctttaaa gccgaccatg ctaatgtgca gccgcacagc ggggcgccgg ctaacgaagc
     2041 tgtctatagc gcgtggctcg aaccgggcga caccgtccta gccatggatc tcagccacgg
     2101 tggacacctg acgcacggcg caccagtgac tcgttcagct cacctgtata actttattcg
     2161 ctacaagatg aaggacccgg ccaccggcga gattgactat gacgaactcc gtgaattagc
     2221 acgcaaacac aaaccaaaaa tcgtgctggc cggcttcagc gcttatcccc gggagttgga
     2281 ttatgccaaa ttcgccgaga ttggtaatga agtcgacgcg ctgctcatgg ccgacatggc
     2341 ccatattgcc ggcctgatag tcgcgggtgt ggccaagaac cctttcgact acggttttca
     2401 tgtcattacg accacgacac acaagacact acgcggtcct cgcggcggct tgattctgag
     2461 ccagggagca gttggcaacc cattcaaggc gccggaaaaa actttggaaa atctgcccac
     2521 cctgattgac cgctcagtgt tccctggcat gcaaggcggc ccgcacgaac atatcattgc
     2581 cgccaaagcc gtagcattcg gtgaggcgct gaagccgaaa tttaaaacgt atgctacgca
     2641 gatcgtcaag aatgcccgag ccctggccga tgctttgcaa gcgcgcggct tctacctggt
     2701 aacgggcggc accagtaatc acttgatact gtccgatgtc cataaaagct ttggtatcga
     2761 cggcgatgtc gtccaggaag tgctcgatag tgtcggcctg acacttaata aaaacgccat
     2821 cgccgacgat ccgttgccgc catcgaagcc cagcggtatc cgacttggta cgccggcaat
     2881 cacgactcgc ggcctagtgg aaactgacat ggaaaagatt gctcaatgga tgaagcaagc
     2941 catcgataac agggaaaata aaacaacact taacaagtta agaaatgatg taaaacaatt
     3001 tgctcttcaa tttcctttgc cgagcgacaa ataacttagt gcgctttgtg ctgccccatc
     3061 ctcatagaat aaagcgtagc taaggcgccg gctccaagca ggctgagcac cgctaggccc
     3121 agaagatttt gacgagataa aggaggtttg tctggctgcg agagaggcag atctggcttg
     3181 ggggtaggtt ggggcttcgt ccattgaaga ctagcaatat cttctttgag ccgctcagca
     3241 aagtcttctt taataatggt gcataagtcg cggtcggtgg ggaattcgtg agcgatttgc
     3301 atgtactctt ggacttcaat ctcaacaact gccgtgttgt cagcgacgtc ggcgagcagc
     3361 ccctttttga gttttgtcag tcggccttgc tcaaagccaa ccttgataag tagcagagcg
     3421 gtgagccggt cttcaaggtt gccgatttcg tggtccgcag caatgacgtt ggccagctca
     3481 ttgaaggatt cgcaagttct cgtttgtgcg tggctgatgg ttgtttcgta atgggcgggg
     3541 tcagtgtcta aataggagtg ggcggcgcat aagttaaagc accaagtgtc aaaagtactg
     3601 gtagcatgga acagtgccaa gccggtaaat tccttgagac gatctccttg attctcggca
     3661 gacatttgat tgcataatat gcttgcggcc ggaagacgtc aatggttggt tagcaatatc
     3721 acacgaagct agggcagtaa ctcgggttcc tgaacgaggg tgatgttaaa tttttgctgg
     3781 acgatgtcga tgattttttg cttgaacttc agcagatcgg cggtgctccg cgcatgctca
     3841 ttgacgagca cgagtggttg tctgggccag gtggccatgc cggtttcttg atcgtgtatg
     3901 tccttgaggc cggcttgctc gataagccag gcggcggaca gcttgacgga gccagtgccg
     3961 acagaccagt gagggatgga atcgtagttg tctgccagat agctgtactg ctcctcggtg
     4021 acgatggggt tggcgaagaa ggagccgaca ttatgtacaa gtgccggatc gggcagtttg
     4081 gcgctacgga tactgat
//
LOCUS       DATNGP010000214         6403 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_1193404, whole genome shotgun sequence.
ACCESSION   DATNGP010000214 DATNGP010000000
VERSION     DATNGP010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6403
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1193404"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="VN778_05615"
     CDS             complement(<1..227)
                     /locus_tag="VN778_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017624752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HXR50472.1"
                     /translation="MQTMSLNTGAKIPGIGFGTWEITPDSEAKQAVLKALETGYRLID
                     TARIYGNERGVGEAIRESGIPRKDLFVTTKL"
     gene            complement(230..889)
                     /locus_tag="VN778_05620"
     CDS             complement(230..889)
                     /locus_tag="VN778_05620"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXR50473.1"
                     /translation="MFKKETPVRITKYVHSCLKVEMPEPINRTVLFDPGVMGEQALDA
                     AKFAHLDDIIITHIHGDHVSVPLLKKLLALFPTVRITSTSEVVKYLAGEGVKATSTPP
                     QAIEFFEAPHEDVVPLATQSEEIGVHYLDKLTHPGDSHSFHETKAILALPMTAPWGTA
                     VKAINLALELKPQYVLPIHDWHWSDAARSQSYDRFEKLLGEQGITFIKLATGEPVVLQ
                     V"
     gene            complement(949..1677)
                     /locus_tag="VN778_05625"
     CDS             complement(949..1677)
                     /locus_tag="VN778_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020509749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HXR50474.1"
                     /translation="MADNNWDFSGLKAIFFNGTLTKSPDESHTDRLIKLSQSIMKKHG
                     VKTELIRSIDHDIATGIWPDMREHGWETDAWPEIFKRVMDADILVLAGPIWLGDNSSQ
                     TKKIIERLYACSSILNPQGQYAYYGKAGGCLITGNEDGIKHCAMNILYSLQHLGYTIP
                     PQADAGWIGEAGPGPSYGDARDDGSVIGLDNDFTNRNTTFMTWNLMHLAKMLKANGGV
                     PAQGNQRSEWDAGCRFDFENPEHR"
     gene            complement(1692..2072)
                     /locus_tag="VN778_05630"
     CDS             complement(1692..2072)
                     /locus_tag="VN778_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015314116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXR50475.1"
                     /translation="MLGENEVCATVGVRDLSAAQKFYGETLGLTKGMESPGGVFYKSG
                     NSGVFVYPTTFAGTNKATYASWTVSDVDGVVSDLKAKGVAFEQYDNMPDTTREGDVHI
                     MGDMRAAWFKDPDGNILNIVNQMG"
     gene            complement(2166..2738)
                     /locus_tag="VN778_05635"
     CDS             complement(2166..2738)
                     /locus_tag="VN778_05635"
                     /inference="COORDINATES: protein motif:HMM:NF014045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HXR50476.1"
                     /translation="MKRWGKKKPATTYAFIDSQNLNLGIQRNGWKMDWRKFRQHLRDK
                     YGVTKAYMFIGYMISNESLYEYMHELGYLVVLKPTVDVTQPRDTSSSNDTEGKNGDDK
                     EKPVVKGNVDAELVLYAMKELPNYERAIIVSGDGDFFSLAEYLDEQHKLAHIMVPNWQ
                     YSSLLKPYERKIVRLDQLRKQLAYHDRKKK"
     gene            complement(2735..3295)
                     /locus_tag="VN778_05640"
     CDS             complement(2735..3295)
                     /locus_tag="VN778_05640"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HXR50477.1"
                     /translation="MLIFTQITHRLWRGLLATILTSLIVLAVGLMTLHFHGERLLSVE
                     TASMVPTFRPGDALIVAPALPKALHIGDVISYISLRDPRVIISHRLIRIDATNGQLVT
                     AGDSLHTDDPPFSTTAVIGRVTAIMPHFGEILNTLRKPSVMVFVVCMPAVIFLYTEFL
                     RLVHFFKRPMYRIHGYQPPSSDRLKL"
     gene            complement(3308..3892)
                     /locus_tag="VN778_05645"
     CDS             complement(3308..3892)
                     /locus_tag="VN778_05645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50478.1"
                     /translation="MKRSEKCKVLVRAAGVLIAVLVVVSGVTFAVLQSQQIKLAGNTI
                     QTATANLLLGSDGTNYSSSQSGYNFSNLVPGGSAMPASGYPIYLKNSGGTPLALKFAV
                     SSTPSNPDNVDLSKVNVIIMPTSGGATQIFTLQALIAANSTGGLSINTPAQFLANDTM
                     KYTIQVSMASDANVGSSATLGNIDFAFTGVAVTN"
     gene            complement(3976..5511)
                     /locus_tag="VN778_05650"
     CDS             complement(3976..5511)
                     /locus_tag="VN778_05650"
                     /inference="COORDINATES: protein motif:HMM:NF024991.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HXR50479.1"
                     /translation="MRQRQALHAMMSGENVFLTGAPGAGKTYVLNEFVKRAERAGKQV
                     AVTASTGIAASHIGGTTIHSWSGLGILDRLTKEDLKRLGASERLAKRYNNTDVLVVDE
                     VSMLHGQRLDMVNQLAKLLRGSEAPFGGMQVILVGDLFQLPPVSRGSTAFDFVHLSAA
                     WQELAPRICYLTEQHRQQGNDELLALLEAMRRNMLTNSHHALLATRLNHQTDEAVLTR
                     LYAHNVDVEAINLRHLAALPGQSTFFERHVKGSKAKVEQLAKGLLTPEILELKPGAEV
                     MFVANNFGEGYVNGSRGRVISIDNDVPLVELTHGKRVLVESYSWSLNEDGKIRAEISQ
                     LPLRLAWAITIHKSQGMSLDAAEIDLGKAFTPGMDYVALSRVRSLDGLYLRAINAMAL
                     TMHPEIYEFDVALRQLSELLAAETPDIAEEATQSLTPKADKLDMDEALFAKLKVWRQQ
                     RAQTENIPAYIIAHNTALEAVAAKPPATPQQLLALPGFGSRKVEAYGTDILAIVSAHT
                     AKS"
     gene            complement(5577..6221)
                     /locus_tag="VN778_05655"
     CDS             complement(5577..6221)
                     /locus_tag="VN778_05655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50480.1"
                     /translation="MRRFKEAVRRDIIGTLFFCFLLGTGFIWLGSAYDWLQMTDGEKI
                     NGTVLWLTALAIFWYAYETYQLKKSTADQVEIQEEIMLNEFLPILVPVRKGPRAVLRN
                     NDLSKLCIRNLGKGPAKYVEIYFGSVKIDMNSSLAGGEEESVKIEATTQQALRQILVD
                     SPASLKMRITYKDIYSRRFQTVNILWDKTGPGQTYTLRQGAWDFKRLTNNSSKP"
BASE COUNT         1496 a         1669 c         1576 g         1662 t
ORIGIN      
        1 caaagtttag tcgtgacgaa cagatccttt cggggaatgc ctgactcccg aatggcttcg
       61 ccgacgcccc gttcattgcc ataaattcgt gccgtgtcaa tgagccgata gccagtttca
      121 agtgccttaa gtaccgcttg cttggcttcg ctatcgggtg taatttccca agttccaaaa
      181 ccgatgccgg gaattttggc tcccgtgttg agcgacattg tttgcatgtt tacacctgta
      241 atacgaccgg ctcgccggta gccagtttta tgaaagtaat gccctgctcg cccagcagtt
      301 tttcaaacct gtcgtagctc tggctacggg ctgcgtcgct ccagtgccag tcgtggattg
      361 gtagcacata ctggggcttc agctccagcg ctaagttgat cgctttgacc gcggtgcccc
      421 agggagcggt catgggtaat gctaagatgg ctttggtttc gtgaaagcta tggctgtcac
      481 cgggatgcgt cagcttgtcc aaatagtgca cgccgatttc ctcggactgt gtcgccagcg
      541 gcacaacatc ctcgtgcggc gcttcaaaaa actcgatagc ctgcggcggg gtggaggtag
      601 ctttaacgcc ttcgcccgct aaatacttga ccacttcact ggtagaggta atccgcaccg
      661 tcgggaatag ggccaataat tttttcagca gcggcacgct tacatgatcg ccgtgaatat
      721 gggtaatgat aatatcatca aggtgtgcga acttagcagc atcgagtgct tgttcaccca
      781 tcacgccggg atcaaagagg acggtacgat tgatgggctc gggcatttca acctttaggc
      841 acgaatggac gtatttggta attctcacag gtgtctcctt tttaaacaac taggctatta
      901 tagcgggcgc ccgagcgggt ctactgtaaa ataactgact gcttttggct agcggtgttc
      961 ggggttttcg aaatcgaagc ggcaaccggc gtcccactcg gagcgttgat tgccttgcgc
     1021 tggcacgccg ccattggcct tcagcatttt ggcgaggtgc atgagattcc aggtcatgaa
     1081 ggtcgtgttg cggttggtga agtcattgtc gaggccgatt acgctgccat cgtcgcgagc
     1141 atcaccatag cttgggcccg ggcctgcttc accgatccag ccggcatcag cctggggtgg
     1201 gatggtgtaa ccaagatgct gcaaactgta gagaatattc atcgcgcagt gcttgatgcc
     1261 gtcctcgttg ccggtaataa gacagccgcc cgccttaccg taataggcgt actgtccctg
     1321 tggattcaag atactgctgc aagcatacag acgctcgata atcttcttag tttgggagct
     1381 attgtcgcca agccagatcg gacctgccaa gacaagaatg tcggcgtcca tgacccgctt
     1441 gaaaatctcc ggccacgcgt ctgtctccca accgtgctcg cgcatgtcag gccagattcc
     1501 agtggcaata tcgtgatcaa tcgagcgaat taactcagtc ttcacgccat gttttttcat
     1561 gatactttgg ctcagtttga tgagtcgatc agtgtggctc tcgtcagggg atttcgtaag
     1621 tgtaccatta aagaagatcg cttttagccc ggaaaaatcc caattattgt ctgccataga
     1681 gtatggtacc tctaacccat ctggttcact atatttaata tattaccgtc tgggtctttg
     1741 aaccatgcgg cccgcatatc gcccatgata tgtacgtcac cctcccgcgt cgtgtccggc
     1801 atattatcgt actgctcgaa ggcaacaccc ttggccttta ggtcactgac cacgccatca
     1861 acatccgaca ccgtccatga cgcataggta gctttattag tgccggcaaa agtcgttgga
     1921 tagacgaaaa ccccgctatt gcctgattta taaaaaacgc cgccgggact ttccatgccc
     1981 ttcgttagcc ccaatgtttc gccgtaaaat ttttgtgctg ctgacagatc ccgtacaccg
     2041 actgttgcac atacttcgtt ttcacccaac ataatgccct cctttggtat ataagttagt
     2101 tgattttaga ggttttggtt tcgccgttta ttatacgctg caaatgctgg ctaggatgta
     2161 tgaatttact tctttttacg gtcgtggtaa gccagttgtt tacgcagctg gtcgaggcga
     2221 acgatcttgc gctcgtaggg cttaagcaag ctggagtatt gccagttggg aaccataata
     2281 tgggctagtt tgtgctgctc gtctaggtat tcggccaggc tgaaaaaatc accgtcacca
     2341 gatacaataa tggcgcgctc atagttcggc agctctttca tggcgtacaa cacgagctcg
     2401 gcatcgacat tgcccttgac aacaggtttt tctttgtcgt cgccgttttt accttctgtg
     2461 tcatttgatg aactcgtatc gcgcggctgc gtcacatcca ctgtcggttt cagtacgact
     2521 aagtagccaa gctcgtgcat atattcgtaa agagattcgt tactaatcat atagccaatg
     2581 aacatataag ctttggtcac gccatattta tcccgcaggt gctgacggaa tttgcgccaa
     2641 tccattttcc agccattgcg ttggatgcca agatttaagt tttggctgtc gataaatgcg
     2701 taggtagtag cgggcttttt cttgccccat cgtttcatag ttttagtcta tcactactcg
     2761 ggggctgata gccatggatt cgatacattg gtcgcttgaa gaaatgtact aagcgcagaa
     2821 attcagtgta tagaaatatg acggctggca tgcacacgac aaacaccata acgcttggtt
     2881 ttctcagtgt atttaggatt tcgccgaaat gcggcataat ggcagtgact ctcccgatca
     2941 cggcagtagt tgaaaatgga ggatcgtccg tgtgcaaact atcccctgcc gtgaccagtt
     3001 gtccatttgt ggcatcgata cggattaagc gatgcgaaat gatcactcgg ggatcgcgga
     3061 ggctgatgta gctaatgacg tcgccgatat gtaaagcttt gggcaaggct ggggcaacaa
     3121 tgagagcgtc gcccggccga aaagtcggca ccatacttgc cgtttcgacg ctgagtaatc
     3181 gctcgccgtg gaagtgcagg gtcatcagcc caacagcgag cacaatcagc gaagtcaaaa
     3241 tcgtggccaa cagtccgcgc cataggcggt gagtgatttg cgtaaagatg agcacggcta
     3301 gctttcatta attggttaca gctacacccg tgaaggcgaa atcaatattg ccgagtgtgg
     3361 cactcgaacc cacgttggcg tcactggcca ttgaaacttg gatcgtatat ttcatggtgt
     3421 cattagctag gaattgcgcc ggggtgttga tgctcaggcc gccggtagaa ttcgcggcaa
     3481 tcagtgcctg gagcgtgaaa atctgcgttg cgccaccact ggtaggcatg ataatgacat
     3541 taaccttact caagtcgaca ttatcgggat tgctcggagt actactaacg gcaaacttaa
     3601 gcgccagtgg cgtaccgcca gaatttttta ggtaaatggg atagccgcta gcgggcatgg
     3661 ccgagccgcc gggtactagg tttgagaaat tataaccact ctgtgaactt gagtaattag
     3721 taccgtcgga gccgagcagc aaattggccg ttgcggtctg gatggtgttg ccggctagtt
     3781 tgatttgctg gctttgtaaa acggcgaagg tgacgccgct gaccaccacc aggacggcaa
     3841 taagtacgcc cgcggccctc accaggactt tacatttttc cgatcttttc ataaaacctc
     3901 ctgacattag tgagtcacag catggcagga gatagatgct tacgccaaaa ttttatggct
     3961 agagttaata aatatttaag atttagcggt gtgagctgaa actatcgcca gaatgtcggt
     4021 gccataggct tccaccttcc ggctcccaaa gcccggtaac gctaagagtt gttgcggtgt
     4081 ggccggcggc ttggcggcga cagcttccag cgctgtatta tgagcgatga tgtaagctgg
     4141 aatattttct gtctgggcac gctgctggcg ccagactttg agcttggcaa agagcgcttc
     4201 atccatatcg agtttgtctg ctttaggggt aagcgactgc gttgcttcct cggctatatc
     4261 aggagtctcg gctgctagca actcggacag ctgacgaagc gccacatcaa attcataaat
     4321 ctccgggtgc atggtgagtg ccatggcatt gatagcacgc aaatataagc cgtctaggct
     4381 tcgcacccgg ctgagcgcca catagtccat gccaggcgta aaagctttgc caaggtcaat
     4441 ctccgcggcg tccaggctca taccctgact cttatgaatg gtgatagccc aagccagtct
     4501 gaggggcaat tgactaatct ccgcgcgtat tttaccatcc tcattcagac tccaactata
     4561 cgactccact aacacacgct tgccatgcgt caattccacc agcggtacat cattatcgat
     4621 cgatatgacc cggccacggc taccgttaac gtaaccctcg ccgaagttgt tggccacgaa
     4681 catgacctcg gcaccgggct tcagctctaa aatttccggc gtgagcaagc ccttcgccag
     4741 ctgttcgacc ttcgctttac tgccctttac atggcgctca aagaaagtgc tctggcccgg
     4801 caaggctgcc aaatgccgca gattgatcgc ctcaacgtcg acgttgtggg catataatct
     4861 cgtcaaaacg gcttcatcag tttggtgatt cagcctggtg gccagtaagg catggtgcga
     4921 attggtaagc atatttcgac gcatggcttc cagcaacgcg agcagctcat cgttgccttg
     4981 ctgccggtgc tgttcggtca aataacagat ccgtggagcc agctcctgcc aggcggccga
     5041 caaatgcaca aaatcaaagg cggtactgcc acggctgacc ggcggcagct ggaatagatc
     5101 gccaaccaga ataacttgca ttccgccaaa cggcgcttct gagcctcgca gtagcttcgc
     5161 cagctgatta accatgtcca accgttgtcc gtggagcatc gacacctcat caaccactaa
     5221 aacatcggta ttgttatagc gtttggccag ccgttcgctg gctcctaggc gcttcagatc
     5281 ttctttggtc agccggtcga ggatacctag ccctgaccaa gaatggatag tcgtgccgcc
     5341 gatatggctc gccgcaatac cagtactggc tgtcactgcc acttgttttc cggcccgctc
     5401 cgcgcgtttc acaaattcat tcaaaacata cgtcttgccc gctccgggag cgcccgtcaa
     5461 aaagacgttc tcaccactca tcatggcgtg taaagcttgc ctctgccgca tcagactccg
     5521 attgtagcac cgagtggaca gccgtccaaa agatgccaca gaaagttaac aaaaatttaa
     5581 ggtttggacg aattattcgt caaccgctta aagtcccacg ccccttgacg caatgtatat
     5641 gtttggccag gcccagtttt atcccataga atgttaacgg tttggaagcg gcggctgtaa
     5701 atgtctttgt aggtgatcct catttttagg ctggccggcg aatcaaccaa aatttgacgc
     5761 agcgcttgct gtgtcgtggc ctcaatcttc acgctctctt cctctccgcc agccaagctg
     5821 gaattcatat ctattttcac gctaccgaag taaatttcga cgtattttgc tggaccctta
     5881 cctaaattcc gaatacataa tttgctaaga tcgttattcc gaagtaccgc acgcgggcct
     5941 tttcttacag gtaccaaaat cggcaaaaat tcattcagca tgatttcttc ctgaatctcg
     6001 acttggtcag ccgtgctctt tttgagctga tatgtttcat aggcgtacca aaaaatagcc
     6061 agggctgtca gccacaacac cgtcccatta atcttctccc cgtcagtcat ctgtagccag
     6121 tcgtaggcac tccccaacca aataaatccc gtgcccagca aaaagcaaaa gaacaaagtg
     6181 ccgataatat cgcgcctaac tgcttctttg aaccgtctca tatataaaca tgataactat
     6241 acaaattaat tgtccgcctg tccattatat cccggccacc tcttggattt tccgcactgt
     6301 ttcggccgcc gaataggctt cagggatgac gcgctcaaaa tcataaccca taaagtcttt
     6361 gggatgatac caatccttca tatcgctttc gctccactcg tct
//
LOCUS       DATNGP010000215         3750 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_2477974, whole genome shotgun sequence.
ACCESSION   DATNGP010000215 DATNGP010000000
VERSION     DATNGP010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3750
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2477974"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1161)
                     /locus_tag="VN778_05660"
     CDS             complement(<1..1161)
                     /locus_tag="VN778_05660"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXR50481.1"
                     /translation="MSKRPPRHKVASHQNWGFVILVPAHNEALVIEDTVRRAMELRAP
                     IHIMVIDDGSTDGTGDLAAAVGNERVHILRRVPPNAKKGKGEALNHAYQVVLPKFGEW
                     FPRVPAENVVITVLDADGYLDPYLFDYISGMLERNPRLGGIQTPVTIRSPESSLWLRM
                     QDLEFVGFSGFVQQARHWFSSIGLGGNGQFIRSTALLKLGSRPWSPALSEDLDIGIRL
                     LLRGERLGFCNLGFVHQQGLTKLRPLLKQRTRWIQGHYQAWKYLPAIWKSREFGLFTK
                     LDLTLYLTLVSSVFIVLVNIGVTAIAALGYISVQSQLMGSMYAVSPYFARAAEIILSI
                     GPALLFAATYNKLSRSKIALTSWPAIIVIFCAYGWIWVYASIVALLRLARRQN"
     gene            complement(1258..1755)
                     /locus_tag="VN778_05665"
     CDS             complement(1258..1755)
                     /locus_tag="VN778_05665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50482.1"
                     /translation="MKLQNKFIMGALSFLILASAGLFVMQPTVAHADAVAQVQFDKKT
                     GQIVMFGGVNLTSGEPEQGVRLSVAATRQNDGGWGHRRSDGRLEILDSTTTDSSGRFK
                     LWVNPQDSRRMHPGVVLTLLLRPHDSHQTYQEDLLLKAGDVASITVAMRTPLVPIFPF
                     TVFVY"
     gene            complement(1739..2872)
                     /gene="wecB"
                     /locus_tag="VN778_05670"
     CDS             complement(1739..2872)
                     /gene="wecB"
                     /locus_tag="VN778_05670"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015791457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HXR50483.1"
                     /translation="MDLAIVVGTRPEIIKMSPVIKACEAQGVGYTLIDTKQHYSDNMS
                     SAFYDDLELRPPDHVLATKTGSHGTQLGSMLPQLEEILQNKPHTTVIVQGDTNTVLAG
                     ALTASRQGVAVAHVEAGLRSRDRTMPEEINRILVDNIAKYLFAPSNETVDNLRQEGIA
                     ETAVWNVGNTIVDATLDGIELAKKKSATDTKPKSDYLLLTLHRPANVDDPVVLKEILA
                     GVAAVMKAYQLPALFPVHPRTRQRIASFGLALPKDLQLIDPVGYLDFLMLESGASLIL
                     TDSGGIQEEACIMHVPCVTLRNNTERPETIMVGANILGGTEQQGIRQAADVMLGLPTS
                     WGIPYGDGKTGERILNILTEASEPYTTNNATTVGARGLYETAK"
     gene            3342..>3750
                     /locus_tag="VN778_05675"
     CDS             3342..>3750
                     /locus_tag="VN778_05675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50484.1"
                     /translation="MFPTDDIFTAPQPERNSTVPQKDAGITPRFTKPSMLTATSTRGE
                     TYRLMIDSAYPLGTPGTAWLNVATATVGNDDRPSFRMVRFGEPSRTHEGELVVPFWAG
                     TRSGTLMQSGGNVHATLTLPDGNRDDVTFTYERN"
BASE COUNT          950 a         1034 c          876 g          890 t
ORIGIN      
        1 gttctgtcgg cgagccaatc gcaggagtgc cacaattgag gcgtataccc aaatccaacc
       61 atatgcacag aagataacaa taattgctgg ccagctagta agcgctattt tggagcggct
      121 tagtttattg tatgtagccg cgaatagcag cgccggacct atcgacaata tgatttcagc
      181 tgcacgcgca aagtatggcg atacggcata catcgagccc attagctggc tctgcaccga
      241 aatgtagccc agcgctgcaa tcgccgtcac accaatgtta actagcacga taaacaccga
      301 gcttactaac gttagataca gcgttaaatc gagcttggta aacagtccaa actcccggct
      361 tttccagatg gccggcagat atttccaggc ttgataatga ccctgtatcc aacgggtacg
      421 ctgcttgagt aacgggcgta gcttagtgag gccttgctgg tgtacaaagc ctagattgca
      481 aaagcccaat cgttcaccgc gcagcagcaa gcgaatgcca atatccaggt cctcagagag
      541 cgccggcgac cacggccggc tgcccagctt taacagcgcc gtggaacgaa taaactgacc
      601 gttaccaccc agcccaatac tggaaaacca gtgccgagcc tgctgtacaa agccactaaa
      661 gccaacaaac tccaggtctt gcatacgcag ccacaatgaa ctttccggcg aacgaatggt
      721 aacgggcgtt tgtataccac ccaaccgtgg attacgctct agcataccgg aaatataatc
      781 aaacaggtac gggtccagat agccatcagc gtccagtacg gtaattacaa cgttttcggc
      841 tgggacacgt gggaaccatt caccaaattt tggcagtact acctggtagg cgtggttcaa
      901 tgcctcgcct tttcctttct tggcattggg tggtacccgg cgcagaatat gcacgcgctc
      961 attaccaact gccgccgcaa gatcaccagt tccgtccgtc gagccatcgt caatcaccat
     1021 aatatggata ggggcgcgca gctccatcgc ccgacgaacg gtgtcttcaa ttactaatgc
     1081 ttcattgtgg gccggtacca ggatgacgaa tccccagttc tggtgcgagg caaccttatg
     1141 acgcggcgga cgctttgaca tgaaaaacag aaatgtatag tacacaaaca cgaagccaaa
     1201 aaaggctacg aagatatcat ccgacacggt cataggcata cctctaaaaa gcgttactta
     1261 gtagacaaat acggtgaatg ggaagatggg cacaagcggc gtgcgcatag ctactgttat
     1321 gctagcaaca tcacccgctt taagcagcaa gtcttcctga tacgtttgat ggctgtcatg
     1381 tgggcgcagc agcagcgtta ggacaacgcc tggatgcatc ctacgtgaat cctgcggatt
     1441 gacccacaac ttaaatcggc cgctgctatc cgttgtggta ctgtctaaga tctctagccg
     1501 gccgtccgaa cgtctatggc cccagccgcc atcgttctgc cgtgtagccg ccacagacaa
     1561 gcgcacgccc tgttctggct caccactagt taggttaacg ccaccgaaca tcacaatctg
     1621 accggttttt ttgtcaaact gtacttgggc aacggcatca gcatgtgcga ccgttggttg
     1681 cattacaaac aatcctgcac tggctagtat caggaacgat aacgccccca taataaattt
     1741 attttgcagt ttcatataac ccccttgcgc ctacagtggt cgcgttgtta gttgtatatg
     1801 gctcggaagc ctcggtcaga atattcaaga tacgctcgcc agttttacca tccccatacg
     1861 gtatacccca gctggtaggc aaccccagca ttacatctgc agcctgcctg attccctgct
     1921 gttcggttcc gcccagtata ttagccccta ccatgatggt ttctggccgc tcggtattat
     1981 tgcgcagcgt tacgcacggt acgtgcatga tgcaggcttc ttcctggata cctccggaat
     2041 cagtcagaat aaggcttgct ccagattcaa gcatcagaaa atccaaatag ccaaccggat
     2101 caatcaattg caaatctttt ggaagcgcca ggccaaacga tgctattcgc tgcctggtac
     2161 gtggatgcac tgggaacaac gcaggtaact ggtaagcctt catgaccgct gcaacacccg
     2221 ccagtatttc ctttaggaca accggatcat caacgttcgc cggtcgatgc agtgtcaaca
     2281 gcaaatagtc agatttgggc tttgtatcag tagcagattt ctttttagct agttcgattc
     2341 cgtcaagtgt tgcatcaact atagtgttac cgacgttcca aacagccgtt tcggcaatac
     2401 cctcttgccg cagattatcg accgtctcgt tggacggggc aaataaatac ttggctatgt
     2461 tatctaccag gatacgattg atttcctccg gcatggtgcg atcgcgtgat cgcaggcccg
     2521 cttcaacatg tgcaaccgca accccctgcc tactggcagt tagtgccccg gcaagcactg
     2581 tattagtgtc cccctgcaca attactgttg tgtgcggctt gttctgtaaa atttcttcaa
     2641 gttgtggcaa catcgaccct agctgcgtgc cgtgcgaacc tgttttggtc gccaacacat
     2701 gatcaggtgg ccttagttcc aaatcatcat aaaatgcgct actcatattg tctgagtagt
     2761 gctgcttggt atcaattagc gtatagccaa caccctgcgc ttcgcaggct tttatgaccg
     2821 gactcatctt aataatctcc gggcgtgtac caacaacaat agcaagatcc atgcagatta
     2881 ctcctttaag cgactgaact tagcggactg agcgttttta gcgagactta tctgcaactc
     2941 tgaaattgtg actactgact ttgttatcgg cttacggcct ggaaagacta gatacagtgg
     3001 taaacgctac ctaattaata agacggtgga ctaaactaaa tacgactcct tatttacata
     3061 cggcccaatt atagtcatct gagccaacaa tgcggtatga cgtttcattt agaattgcag
     3121 acttatgtga tagaatgcgc cccttcatag aagctgtact ttcgcccgcc tactcacctc
     3181 ttacacacag accggcttgc ctcacggcac ctgtaaattg caaatactgc ataaacattc
     3241 tataatacta gtgtttcggg ctgacgtttt ggccttgaag cactttaacg cctcctgtct
     3301 cgtgttgtcg gttggaactg aatcataacg gcccttatga tttgtttcca actgatgaca
     3361 tattcactgc accacaaccc gaaaggaaca gcacagtgcc ccaaaaggac gcaggtatca
     3421 ccccacgttt taccaaaccg tctatgctca ccgcaacaag cacgcgcggc gagacctacc
     3481 gcctgatgat cgactcggcg taccccctcg gaacaccggg caccgcctgg ctcaacgtcg
     3541 caacggccac cgtcggcaac gacgacaggc catccttccg gatggtgagg ttcggagagc
     3601 cgtcgcgcac ccacgagggt gagctagtgg ttccgttctg ggcgggtact cgcagtggca
     3661 cgctgatgca atccggcggt aatgtccacg ctacgctgac actcccggac ggcaaccgcg
     3721 acgacgtgac attcacgtac gagcggaacg
//
LOCUS       DATNGP010000216         7358 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_3661527, whole genome shotgun sequence.
ACCESSION   DATNGP010000216 DATNGP010000000
VERSION     DATNGP010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 7358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7358
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3661527"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1547
                     /locus_tag="VN778_05680"
     CDS             <1..1547
                     /locus_tag="VN778_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013181446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HXR50485.1"
                     /translation="MAVIGSGPYSIGSSVEFDWCAVNTAKSLHAHGHQTIIINSNPET
                     VSTDFDRSDRLYFDELTLERIQDIADFERPKGLVVSVGGQIANNLALPLMKAGYPILG
                     TSPERIDHAENRQKFSAMLHDLGVDQPAWREVTSLKNAKQFATHVGYPVLIRPSYVLS
                     GGAMNVVRNEEEMEQYLQEATRLSPDHPTVISQFILNAKELEIDAVAKNGKLVIYAIS
                     EHIENAGVHSGDATVVYPAQRLYLETVRRAKQIARQVASSLDITGPFNIQFIAKDNEL
                     KVIECNVRASRSFPFVSKVSGHNFITIATEAMLGIENNTVYQTLELDHVGIKSPQFSY
                     NRLKGADPVAGVEMASTGEVACFGRDIEEAFYTSWLATEQQVGGKNVFISLPDEQKHK
                     FVEEVAALAAQGWNIYSTAGTHRYFKKHGIPTTKLYKISDKKEPSVHNAVEQRGLDLM
                     ISVPAAEEALTETNNDGYKIRRLAIDHHIPLFTNAESSRLMLGCLTNPALQDLQPRYW
                     REYVPA"
     gene            1687..2190
                     /locus_tag="VN778_05685"
     CDS             1687..2190
                     /locus_tag="VN778_05685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50486.1"
                     /translation="MGKIIELSALVSLLFVFVCSVAFPRSSFMWLATASSTFTILRMI
                     LITLIATLLITRSFFDHHLHWLLGAFAIMLSGWALFATYSNQLQILDGLSFLAAGLSL
                     GIEALEPGVDRPSTEPLSLQQLRSEVKHKLAIYTLAAYVVTRAIALNHSPNNQLTMHG
                     GHRHSAA"
     gene            2328..2744
                     /locus_tag="VN778_05690"
     CDS             2328..2744
                     /locus_tag="VN778_05690"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HXR50487.1"
                     /translation="MEGYTIVDAREVRDAYAGTAVPGAFRSLTSALQSEQLAVTLIHI
                     PPHSDFEQGTGHYHNELEEIYIISRGTLTMRFKDDTHKVSAGSVVRVAPKTPRSHRNE
                     GDEPVDMWAISRIHSHGDATKLDSFWEASKDARQQA"
     gene            complement(2741..3151)
                     /locus_tag="VN778_05695"
     CDS             complement(2741..3151)
                     /locus_tag="VN778_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018620579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXR50488.1"
                     /translation="MSDITLDPYLFFSGNCREAMEFYKSVFGGELSIQTFGDVPGTEA
                     VNKDQIMHANLDGGAVKLFGSDSSKASERAAKIELTIGGSDEAKLTDIFNALSDGGTV
                     KTPLKKEFWGDTFGQLTDKFGIDWMVNIAAPKAD"
     gene            complement(3182..3787)
                     /locus_tag="VN778_05700"
     CDS             complement(3182..3787)
                     /locus_tag="VN778_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005040320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HXR50489.1"
                     /translation="MGRLIVSEFLSLDGVFEDPGGSEGTEHGGWQIPLAGQEAMQYKG
                     EELKAADALLLGRVTYEGFAKAWPTIEGAGEFGKKMNSMPKYVVSSTLTADELTWENS
                     SLVEGDLVEEIHKLCKQFDHDILVAGSGKLVRALMEHDLVDRYRFMTHPVILGGGRRL
                     FDGEAKKTMRLTDSRIFSSGTVVLEYRQIKENLPEALNSKV"
     gene            complement(3866..4366)
                     /locus_tag="VN778_05705"
     CDS             complement(3866..4366)
                     /locus_tag="VN778_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005809631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXR50490.1"
                     /translation="MQKISASLWFDKNAEEAMNFYVSVFEAAPRAKAGESEITTIKRY
                     PGGYTEGPLANMEGKVLTGVFQLAGQQFMALDGGPLFKFNEAVSLVVECEDQAEIDYF
                     WGKLSAVPESEQCGWLKDKFGVSWQIVPNNMDELMQIPGAMDALLKMQKLNIAELKAA
                     GQPPKS"
     gene            complement(4378..5181)
                     /locus_tag="VN778_05710"
     CDS             complement(4378..5181)
                     /locus_tag="VN778_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HXR50491.1"
                     /translation="MQTVTSKDGTKIAYDKTGQGPAVILVAGAMGTRSFGHADLAKLL
                     SSSFTVYNYDRRGRGDSTDTQPYAVEREIEDIEALIDEAYGTACLYGISSGACLVLEA
                     AFRLGDKVNKIALYEPPYNSDPADRPIWQAYYTKLKDLVVAGQRGKAVEHFMKFLQVP
                     DNMIEGMRKQPMWPGLEAVARTLIYDAQAMGVDTRAVPTELAKKVKVPALVMDGEETF
                     AMYPFMRASADELARALPNAKRQTLEGQRHDVSSEVLAPVLTKFFTQES"
     gene            complement(5193..5669)
                     /locus_tag="VN778_05715"
     CDS             complement(5193..5669)
                     /locus_tag="VN778_05715"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HXR50492.1"
                     /translation="MAEENERELTLTRIYDAPRELVWRFWTDPELLARWWGPHGVTNP
                     ESEVDLRPGGVIRIVMLAGEEMGPLAGQRWPMQGMFKEIVEPEKLVFANQAVDEAGNV
                     LIDGMTTVTFEEQDGKTRLTMQTRAKAVTPEAVPMIEGMTAGWTQSLDKLTEAIKQ"
     gene            complement(5671..6003)
                     /locus_tag="VN778_05720"
     CDS             complement(5671..6003)
                     /locus_tag="VN778_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007904002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HXR50493.1"
                     /translation="MNLDTVFGSLADPTRRDILRRVARREQSVGQIAQHYDLTFAAVS
                     KHLQVLEKAKLVTKRRRGKEQMVNIAPGALASADEYLEQYRELWERQLDRLEDLLEQE
                     QHKGKVGD"
     gene            6138..6791
                     /locus_tag="VN778_05725"
     CDS             6138..6791
                     /locus_tag="VN778_05725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50494.1"
                     /translation="MSLISKEKLPVLATTGVLLAGATPAMAATSGSHNKPNLVKEVQR
                     DVNQLAHRAYTDKKLGRMALSLSKQGRTVTVTLQESAPTHTGSDTGGEYKIRFIADIA
                     RSGGPNLNRVHKLIISEGTTNHNNQLIDGTLLEAEVTPGNARIVEENAPFNVTTPVIS
                     EAAVHPLPSELGARAKWVPMWDGLFNRLITHAENQTPTGPQPHMIFGEPGGEQALPV"
     gene            complement(6803..>7358)
                     /locus_tag="VN778_05730"
     CDS             complement(6803..>7358)
                     /locus_tag="VN778_05730"
                     /inference="COORDINATES: protein motif:HMM:NF017959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HXR50495.1"
                     /translation="AAAWTRAHYSWLHNTISDLGNTVCGPYGTWLVCSPLHMLMNASF
                     IVLGSTMILGSLLVYHEFQRSTGSAIGFSFMAMAGLGTALVGLFPENTVSALHVLGAA
                     LPFILGNFSLLVLGKSLTLPRALRVYTVLSGGIALIALGFFVTHTYLGIGQGGIERIT
                     AYPQTIWLIVFGAYIARDHYLRQR"
BASE COUNT         1785 a         2068 c         1680 g         1825 t
ORIGIN      
        1 ctatggcggt catcggctcc ggaccgtatt cgatcggctc ctcagtggag tttgactggt
       61 gtgcggtcaa tacagccaag tccctccacg cccacgggca tcaaaccatc atcatcaact
      121 caaatccgga gaccgtctcg accgacttcg accgctccga taggttgtat tttgacgagt
      181 taaccctgga gcgcattcaa gacattgccg attttgagcg acccaaaggt cttgtcgtct
      241 cggtcggtgg ccaaatcgcc aacaacctag cactgccgct tatgaaagct ggctatccga
      301 ttcttggtac ttcgccagag cgcattgatc acgccgagaa ccgccaaaag ttttccgcca
      361 tgctgcacga cttgggcgta gaccaaccgg cttggcggga agtcacaagt ctaaaaaacg
      421 ccaaacagtt cgctactcac gtcggttacc cagtattgat ccgcccgtcc tacgtccttt
      481 caggcggggc catgaacgtc gtgcgaaacg aagaagagat ggaacagtac ctgcaggagg
      541 ccactcggct atcaccggat catccgacgg tcatttcaca gttcatattg aatgccaaag
      601 agctcgaaat agacgctgtg gcaaaaaacg gcaagctggt aatctacgcc atctctgagc
      661 acatcgaaaa tgctggcgtg cactccggcg atgcgaccgt cgtctatccg gcgcaacgcc
      721 tatacttgga aaccgttcgc cgggccaaac aaatagctcg ccaagttgcc agcagcctag
      781 acatcaccgg gccattcaac attcagttca tcgccaaaga caacgaacta aaagttattg
      841 aatgcaatgt ccgggcatca cgctcattcc cgtttgtatc aaaagtatcg gggcataatt
      901 tcataactat cgccacggaa gccatgctcg gcattgaaaa taataccgtg tatcagacgc
      961 tagaactcga tcacgtcggc atcaaaagcc cgcagttttc atataatcgt ctgaaaggcg
     1021 ctgatccggt cgccggtgtc gaaatggcct ccacgggtga agtggcttgt ttcggccgag
     1081 acatcgagga agcattttat acttcttggc tcgctaccga acagcaggta ggtggcaaaa
     1141 atgttttcat tagtctgccc gacgagcaga aacataaatt cgttgaggaa gtggcagctc
     1201 tggcggcgca gggttggaat atttactcga cagcaggcac gcatcgttac ttcaagaagc
     1261 atggcatacc caccactaaa ctgtacaaaa taagtgacaa aaaagagccg agcgtccata
     1321 acgccgtaga gcaacgcggc ctcgacctaa tgattagcgt gccggcagcg gaagaagcac
     1381 ttacagaaac gaataacgat ggttataaga ttcgtcgcct ggccattgac caccacatcc
     1441 cgctttttac gaatgctgaa agcagccggc taatgcttgg ctgcctcacc aatcctgccc
     1501 tccaggatct ccagccacga tattggcgag aatacgtgcc ggcttaaaat tcaccgaatt
     1561 ttcacccctt gctaagctgt caaatctata tctttgtaag tttacttaca gtcgagccat
     1621 agctgagtag cgttaaatgt agttagacaa gttactggca atctgagcaa gaagaggagg
     1681 tgttagatgg ggaaaattat tgaactttct gcgcttgtta gcctactgtt tgtgtttgtt
     1741 tgttcggtcg cttttccgcg cagttcgttt atgtggctgg ccacagcttc ctcaactttc
     1801 accatcctga ggatgatcct tattacctta attgccaccc tgctcatcac tcgttcattt
     1861 tttgatcatc atttgcactg gctgctgggc gcttttgcca taatgttaag tggttgggca
     1921 ttatttgcaa catatagcaa ccagctgcag atacttgacg gcttatcatt tctagccgcc
     1981 ggcctttctc tcggtattga agccctggaa ccaggtgtcg atcgccccag caccgaacct
     2041 ctctccttgc agcagctgcg atctgaggtg aagcataaac tagctatcta caccttggcc
     2101 gcctatgtcg tgaccagggc aattgcactg aaccattcgc caaataatca actgacaatg
     2161 catggcggac atcgacactc ggctgcatga ctcgcagcct ttcccccgcc gaaaaaagca
     2221 ttatcggact acttggcttc gcgagttttg gcctgaaatt agctgagtaa ttgttgtaat
     2281 aatttttgtc gctgcttctc tcgtgaacaa gtactttagt gagtagtatg gaagggtaca
     2341 cgatagttga tgcgcgggaa gtcagggatg cttatgctgg cactgctgtg ccgggagcat
     2401 tccggagcct gactagtgct ttgcaaagcg agcagctggc agtcaccttg attcacattc
     2461 cgccgcattc ggatttcgag caaggcacag gacattatca taatgaattg gaagaaattt
     2521 acattatcag ccgcggcacg ttgactatgc gtttcaagga tgacactcat aaagtctcgg
     2581 ccggatcggt tgtgagggtc gcgccgaaaa caccgcgctc gcaccgtaat gaaggtgacg
     2641 agccggtcga tatgtgggcc atctcacgca tacacagtca tggcgatgca acgaagctag
     2701 acagtttttg ggaagcgtcc aaagacgccc ggcaacaggc ttaatcagct ttcggcgctg
     2761 cgatattcac catccagtcg ataccgaact tatcggtcag ctgcccgaaa gtgtcacccc
     2821 aaaactcttt tttcaaaggc gttttaacag tgccgccgtc gcttaaagca ttgaagatat
     2881 cagtcagttt tgcttcatca ctaccaccga tggttagctc gattttggcc gcccgttcgc
     2941 tcgcttttga ggaatcactc ccgaacagct tcacagctcc cccgtctaga ttggcgtgca
     3001 taatttgatc tttgttgaca gcttcggtgc cgggcacgtc accaaaggtt tggatcgaca
     3061 gctctccacc gaagacggac ttgtaaaact ccatcgcctc ccggcaatta ccgctaaaaa
     3121 acagatacgg atctagtgtt atgtcgctca tggcccctcc ttgtttaatg atagctttat
     3181 tttacacctt actattcagt gcttcaggca agttttcttt aatttgccgg tactctaaca
     3241 ctactgtccc agagctgaat atccgggaat cagttagtct catcgttttc ttggcctccc
     3301 catcaaacag gcgccggcct ccgccgagaa taacgggatg agtcataaac cgataccggt
     3361 cgactaagtc gtgctccatc aaagctcgca ccagcttgcc gctaccggcc actaagatgt
     3421 cgtggtcgaa ttgcttacaa agcttgtgta tttcctccac cagatcacct tcaaccagtg
     3481 aggagttttc ccaagtcagt tcgtcggcgg tcaaggtgga ggagaccacg tattttggca
     3541 tactattcat ctttttacca aattcaccag ccccctcaat tgttggccag gctttcgcaa
     3601 aaccttcata ggtcacgcgc cccagcaaca gggcatccgc agctttcagc tcctcgcctt
     3661 tatactgcat ggcttcctgg cccgccaacg gaatttgcca gccgccatgc tccgtacctt
     3721 cagatccacc aggatcctca aaaacgccat ccaaactcag aaactcggaa actatcagtc
     3781 tacccatgcc acctcctttc ggcttctagc cggaagatta caaacttttc ctcgggcgga
     3841 gcaatactat ttctaactgt aaatattaac tttttggggg ttgtccagct gctttgagct
     3901 cagcaatatt aagtttctgc attttcagca aggcatccat ggcccccggt atctgcatca
     3961 gttcgtccat attgtttgga acaatctgcc atgacacccc gaacttatct tttagccaac
     4021 cgcattgctc agactcgggc acagctgaaa gctttcccca aaagtaatct atttcggcct
     4081 gatcctcaca ctctacaacc agcgagacag cctcgttgaa cttgaaaagc ggtccgccat
     4141 ccagcgccat aaactgctgg ccggccagct gaaaaacacc cgtcaaaacc ttaccttcca
     4201 tgttagctag cggaccctcg gtatagccgc ccgggtaacg tttaatagtt gttatctcgg
     4261 actccccggc tttggccctc ggggcagctt caaaaaccga cacgtaaaaa ttcatggctt
     4321 cttcggcatt tttgtcgaac catagtgaag ctgaaatttt ttgcatagta atctcctcta
     4381 actttcttgg gtgaaaaatt tcgtcaacac cggcgctagt acttccgagc taacgtcatg
     4441 acgctgccct tcaagtgtct gccgctttgc atttggtaaa gccctggcca attcgtcagc
     4501 gctggcacgc ataaacggat acatggcaaa cgtttcttcg ccgtccatca cgagcgccgg
     4561 caccttaacc ttcttagcca gctcggtcgg caccgcccgg gtgtccacac ccatagcttg
     4621 ggcatcgtag atgagcgtgc gtgctacagc ctcgagccct ggccacatcg gttgtttacg
     4681 catgccctca atcatgttgt cgggcacctg cagaaacttc atgaaatgtt ctacagcctt
     4741 cccgcgctga ccagcgacta ccaagtcttt caatttcgtg tagtacgcct gccagattgg
     4801 cctatcggcc ggatcagaat tataaggcgg ctcatacaag gcgatcttgt tcaccttatc
     4861 gcccagtcgg aacgccgctt ccagcaccag acaggcaccc gacgaaatac catacaaaca
     4921 ggccgtaccg taagcctcat cgatgagcgc ttcgatgtct tcaatctcac gctcgacggc
     4981 atagggctgc gtatcggtgc tgtcgccccg tccgcgccga tcatagttgt aaacggtaaa
     5041 gcttgagctc agcaatttgg caaggtcggc gtgaccaaac gaacgtgtac ccattgctcc
     5101 tgccactaaa atcacagctg ggccttgtcc tgttttgtcg taggcaattt tagtgccgtc
     5161 tttagacgtt acggtctgca tatgagcctc ctttactgtt ttattgcttc tgttagttta
     5221 tccaaggact gcgtccagcc tgccgtcatt ccctcgatca ttggcacagc ctcaggcgtt
     5281 acagccttgg ccctggtttg catcgtcagc ctggttttac cgtcttgctc ttcgaacgtt
     5341 actgtggtca tgccatcgat taagacattc cctgcttcat ccactgcctg atttgcgaaa
     5401 accagctttt cgggctccac gatttcctta aacatgccct gcatcggcca gcgctggccg
     5461 gctagcggac ccatttcttc gccggccaac ataacaattc gaatgacgcc gcctggccgc
     5521 aggtcaactt cgctttcggg gtttgttacg ccatgcggac cccaccaccg tgccaggagt
     5581 tccggatctg tccagaagcg ccacaccaat tcgcgtggtg cgtcgtatat tctcgttagg
     5641 gttagttctc tctcattttc ctctgccata ttaatctcct acctttcctt tatgttgctc
     5701 ttgctctagc aagtcttcaa gccgatctag ctggcgttcc caaagctccc ggtattgctc
     5761 taggtactcg tcggcgctcg ctaaagcccc tggtgctata ttgaccattt gctctttgcc
     5821 cctccttcgc ttcgttacta gcttagcctt ttccagcact tgaagatgct tggacacagc
     5881 cgcaaaggtt aagtcgtaat gctgggcgat ctgccccaca gactgctctc ggcgagctac
     5941 tcggcgcaaa atatcccttc tagtcgggtc agccaggctg ccaaatacag tatccaaatt
     6001 catcgtatat tcaaccatat agttgaatga tacgctccgc atctgtgact gtcaagtgtg
     6061 agtgcaaatt atccttgaca aaacgcttat ttttaatgta atattagtgc tttgtgtaac
     6121 tgaaaaggag aaccttcatg tctttaatct ctaaggaaaa actccctgtc ctcgcgacaa
     6181 ccggtgtact cttagcgggt gcaactccag ccatggctgc cacttcgggt agccacaaca
     6241 aacccaatct tgttaaagaa gttcaacgtg acgttaatca attagcccac cgggcctaca
     6301 ctgacaaaaa actaggccgc atggccttga gcctctccaa acaaggtcgc accgtaacgg
     6361 ttactcttca agaaagcgcc ccgacgcaca caggtagcga tacagggggt gagtacaaaa
     6421 tccgcttcat agccgacatt gcacgtagcg gcggcccgaa tctgaaccgt gttcataaac
     6481 tcataatatc cgaaggcacc acgaatcaca acaatcaact aattgatggc accttgctgg
     6541 aagccgaggt aacgcccggt aacgcacgca ttgttgaaga gaatgcgcct ttcaatgtca
     6601 ccacccctgt catcagcgag gctgctgttc atcccctccc ctctgagctc ggtgcccggg
     6661 ccaaatgggt tcccatgtgg gatgggctct tcaaccgcct cattacccat gccgagaatc
     6721 aaactcccac tggcccccag cctcacatga tcttcggcga gccgggcggc gagcaagcac
     6781 tcccggtcta attccaggtc tcttaccttt gtcgcaaata gtgatcacgg gcaatatagg
     6841 caccaaacac gataagccaa atagtttgcg gataggctgt aattcgctcg atgccacctt
     6901 gccctatgcc gagataggtg tgtgtcacaa aaaatccgag agcaatcagc gcaatgccac
     6961 cagacagcac agtataaacg cgtagagctc gtggcaatgt caggcttttg ccgagtacta
     7021 acaagctgaa attaccgaga ataaatggca aagcggcgcc taaaacatgg agcgctgata
     7081 cggtgttttc tgggaatagc ccgacgagtg ccgtgccaag accagccatc gccatgaagc
     7141 tgaaaccgat cgccgagccg gtgctgcgct gaaactcgtg atagaccagt agcgacccta
     7201 gaatcatcgt actgccgagc acgataaatg aagcattcat tagcatgtgg agcggcgaac
     7261 aaacaagcca cgtaccatat ggaccgcaaa cggtattacc cagatcactt atagtattgt
     7321 gcagccaact ataatgtgcc cgcgtccatg ctgcagcc
//
LOCUS       DATNGP010000217         4285 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11324
            k141_3359941, whole genome shotgun sequence.
ACCESSION   DATNGP010000217 DATNGP010000000
VERSION     DATNGP010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945896
            Sequence Read Archive: SRR6185512
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:08:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,150
            CDSs (total)                      :: 1,109
            Genes (coding)                    :: 1,102
            CDSs (with protein)               :: 1,102
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4285
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3359941"
                     /isolate="SMAG_U11324"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            275..706
                     /locus_tag="VN778_05735"
     CDS             275..706
                     /locus_tag="VN778_05735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50496.1"
                     /translation="MGATLAIVALCGTSLAVWAFRSHDTTATSPTHRSTTYQANKIPA
                     DGHLLMPTLKGNGSSRSAVFDAPSSWQVYWSFVCYSEDQLGSLHITPTAANGLPVAGN
                     KPITAQGISGFGDQPYQLGGRLYLSIQAAAGCTWQLTVKAP"
     gene            764..1594
                     /locus_tag="VN778_05740"
     CDS             764..1594
                     /locus_tag="VN778_05740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50497.1"
                     /translation="MQHRKLLASFLWRFAVCLTAISLLYLLVFGLPKKAAKRSSTNTQ
                     RLASIRAALDETYIAGRSLATLHQTDASAYEALNDLHDEYQTAAADLAKAVQQSPATL
                     TSGEQAALQAIVSRNQQASASYTSTYAVLNQVVRYNPSDDLNLPLNSAATEVAARASA
                     AQRGLTKAATPAPVKMATGNSGVQTGIPASFDSTLQAALQAEAQCFGQLASRLNAAHT
                     LAAAGQTRTACLQTYPALRLEALQAVTGAAFTQEYQDYLDQTVPPLLKQMDSQIRSRH
                     "
     gene            complement(1604..2293)
                     /locus_tag="VN778_05745"
     CDS             complement(1604..2293)
                     /locus_tag="VN778_05745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50498.1"
                     /translation="MGQLKHFVLRPRWRPLTAWLLPLVLSLGLMFGVHAAVTADTPPT
                     TDQLAQYLQNNALDVTTDTVDGYQQVYYHYQGQKVYITGAVYNHTHPAASGEYVVWEG
                     LADNHGQIYLYDVLTDALIRIVSPGTSENPSLFQDSVVWEEWLNDSWQIFYYNGFTIQ
                     QVSVDNVPSVRPSINAHQVIYAEQFPTYWQTISYDIASGQRTVIMQGDESSTAYPHFA
                     PDGTIRTGINP"
     gene            complement(2281..4068)
                     /locus_tag="VN778_05750"
     CDS             complement(2281..4068)
                     /locus_tag="VN778_05750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXR50499.1"
                     /translation="MKKIHSAGKFRWTCRLANQGRQVGRQVMVGGGVLLITFFNLLLY
                     LPGHAGATPTHMMVFWDPAFGSTPSGWTTLTTFNGDFPRGDTVANYGTTGGSGAPYTP
                     TINTVTVGGPSATIPSPGTGSNSASDTHTHPAPTVTVGSDSNGDIPAYRSLELMEYTT
                     GIPTFLPGGAIVMFDTNGSIPSSGFTRISAQDNLMIQVNSSVTTGGSDTETNSVSVGA
                     LSASTDVVAQAKQQGNATQAAATNHTHAAAASGNTTATSTLPPYVQPQLAQANADTST
                     IPANTVVMFDGDPGTGWQVVSGTGGAYNQQFLRPAATASLTSQGATTHTPPSYIATSG
                     ADQTAGFNAKSNGSGSGSANATHTHQITVIFNSVSNLPPYFNVVIAQKITFSLQAYRW
                     YVESGSQDVTDPWPSGSLDLAQNTMLPAIPAPYNPPDSVAGTQLRLRVQILVSGQALP
                     ASNTQFKLQYKQDTDSTCTTGSWTDVATGNVWSYGANAVTDGTTLTTSRLTPTSSVME
                     IFSKSATSGTNPNAISTGQTAEYDWLIKDASAAGGSQYSFRVVESSGNPLSTYSQCPT
                     VVTAAQVNQDMRHGEFFQDGVDQGFEWAN"
BASE COUNT          934 a         1122 c         1238 g          991 t
ORIGIN      
        1 gtcttgaatt tttttgcgtg gttttggttt catgagtgaa attcccttta gtgccttacg
       61 cttcggaaaa cgcgacagat catggacaaa ttttgattat tttttgcggt ttttgatctt
      121 tgaagatctg aatacattat aatggtagag catggccaag taaaggataa gttgggctgg
      181 cgagcaaata tttataatca tactcttgag aacgaacaca ctgacaaaat ttaggagccc
      241 taataggcag caccacaagc ctataacagc gcgcttgggc gccacgctag ctattgtggc
      301 cctctgtggc acgagcctcg ccgtttgggc tttccgctcc cacgatacga ccgctacgtc
      361 gccgacccac cggtcgacaa cgtatcaagc aaataaaatc cccgccgatg gccatctgct
      421 tatgccgacc ctgaaaggca acggtagtag tcgcagcgcc gtttttgatg caccctccag
      481 ctggcaagtc tattggtcgt ttgtctgtta tagcgaggac caactgggaa gcttacatat
      541 cacacctacc gctgctaacg gtctgcccgt cgcgggcaac aaaccgatca ccgctcaggg
      601 aattagtggc tttggtgatc agccttatca attaggaggc aggctttacc tgtccataca
      661 ggcagcggcc ggctgcacct ggcagcttac ggtaaaagcg ccttgagaag cggcacgccg
      721 ttcgccagga atgctccgaa agggtgctac aatctaggag accatgcagc accgtaaact
      781 cctcgcgtca tttttgtggc gatttgccgt ttgtctgacg gctataagcc tgctctacct
      841 gctcgtgttc ggcttgccga aaaaggcggc gaaacgttcg tccaccaaca ctcaacggct
      901 cgcatctata cgagcggcac ttgatgaaac gtatatcgcg ggcaggtcat tggcgacctt
      961 gcatcaaact gacgctagtg cctacgaggc gctcaatgat ctgcatgacg aataccagac
     1021 agctgccgcg gatttagcga aagccgttca acaatcgcca gccacgctaa ctagcggcga
     1081 acaggctgct ttgcaagcta tagtaagccg caaccaacag gcttcggcga gctataccag
     1141 tacttatgcg gtgcttaatc aagtcgtgcg ttataaccct tcggatgatc ttaatctacc
     1201 gcttaattcg gctgctaccg aggttgcggc tcgggcttcg gcggcccagc ggggattgac
     1261 taaagcagct actcctgccc cagtcaaaat ggctaccgga aactctgggg ttcagacagg
     1321 catcccggcc tcattcgata gcaccctaca agccgccctg caagcggaag ctcagtgttt
     1381 cgggcaatta gcgagccgtc tgaacgctgc tcacactctg gctgctgccg gacaaacaag
     1441 aactgcctgt ctccagactt atccggcgct ccgcttggaa gctttgcagg ctgtcaccgg
     1501 tgctgctttt acccaagagt accaagatta cctggaccag acggtcccgc cgttgctcaa
     1561 gcagatggat tcgcaaatca gaagtcgtca ctaaatctgc ctactagggg ttgatgccgg
     1621 tgcgaatcgt gccgtccggg gcaaaatgag gatatgccgt cgatgactcg tcaccttgca
     1681 tgatgactgt gcgctggcca ctcgcaatat catagctgat ggtctgccaa taggtgggaa
     1741 attgctcagc ataaataacc tggtgggcat tgatactggg ccgcaccgag ggaacattat
     1801 cgacactcac ctgctggatg gtgaagccat tgtagtaaaa gatttgccag ctgtcgttca
     1861 gccattcttc ccagacgaca ctgtcctgaa acagtgatgg attctcgctt gtcccgggag
     1921 atacgatccg gattaaggcg tcagtcagaa catcatatag ataaatttga ccgtggttgt
     1981 cggctaggcc ctcccagact acgtattcgc cgctggcagc cggatgagtg tggttataga
     2041 cggcaccggt gatatagacc ttctggcctt ggtagtggta gtaaacctgc tggtagccgt
     2101 cgacggtgtc agtggtgacg tcgagcgcat tattttgcag gtactgcgcc agctggtccg
     2161 tggtgggcgg ggtgtcggcc gttaccgcag cgtggacgcc gaacatgagg cccaagctca
     2221 gcaccaacgg aagcaaccag gctgtcagcg gccgccaccg aggtctgagc acgaagtgct
     2281 ttaattggcc cattcgaaac cttggtctac gccgtcctgg aagaactctc catgacgcat
     2341 atcttggtta acttgggcag cagtgaccac cgtcggacac tgcgaatagg tactgagcgg
     2401 gttgccacta gactcaacaa ctctgaagct gtactggctg ccgccagcgg ctgaggcgtc
     2461 tttgatcagc cagtcatatt cggcggtttg gccagtagag atggcattgg gattagtgcc
     2521 ggaggtggcg gacttgctaa agatttccat aactgaagaa gttggggtca agcggctggt
     2581 tgtcagcgtc gtgccgtcgg ttacagcatt tgcgccatag gaccaaacgt tgcccgtggc
     2641 gacatcggtc caactgccag tagtacaggt gctatctgtg tcctgcttgt attgcagctt
     2701 gaactgagtg ttgctggcgg gcaatgcttg gccgctgact aaaatctgca ctcgtaagcg
     2761 cagttgggtg ccagcgacgc tgtcgggcgg attgtacggc gccggtatag ccggcaacat
     2821 ggtgttctgg gcgagatcca ggctgccaga tggccagggg tcggtgacgt cttgcgagcc
     2881 agactccacg taccagcgat aggcctggag gctgaaggtg attttctggg caatgacgac
     2941 attgaaatac ggcggcagat tgctaaccga gttaaagata acggtgattt ggtgtgtatg
     3001 ggtagcgtta gctgaacctg atccggaacc attacttttt gcattaaagc ccgcggtttg
     3061 atccgcgccg cttgtagcaa tgtagcttgg tggagtgtgc gtggtagcac cctgggaagt
     3121 caggctggca gtggctgccg gcctcaagaa ctgctgatta tacgcgccgc cagtgccaga
     3181 cactacttgc caaccggtgc cggggtcacc gtcgaacatg actaccgtgt tggctggaat
     3241 ggtactggta tcggcgttgg cttgcgctag ttgtggctgg acgtagggcg gcaatgtcga
     3301 agtagccgtg gtattaccgc tcgcggcagc ggcatgggta tggttggtgg cggcagcttg
     3361 agtagcatta ccctgctgtt tggcttgggc aactacatca gtgctagctg ataaggcgcc
     3421 gacgctcacc gagttcgtct ccgtgtcgct gccgccggtg gtaactgagc tgttaacctg
     3481 gatcataagg ttatcttgag ctgagatgcg cgtgaagccg ctactgggga tactgccgtt
     3541 cgtgtcaaac atcacgatgg cgccaccagg gaggaaagtc ggaataccgg tggtgtactc
     3601 catcagctcc aggctacggt aggcggggat gtcgccattg ctgtctgagc caaccgttac
     3661 cgttggcgcc gggtgggtgt gcgtgtcact ggcagagttg gagccggtac ctggtgacgg
     3721 gattgtagcg ctggggccac cgaccgttac cgtattaatc gtgggggtgt agggcgcacc
     3781 gctgccaccg gttgtaccgt agttggcgac cgtatcaccc cgggggaaat cgccgttaaa
     3841 ggtcgtcaag gtagtccagc cgcttggcgt tgagccaaag gcgggatccc agaagaccat
     3901 catgtgcgtg ggcgtagcac cggcatggcc agggaggtac aacaacagat tgaagaaggt
     3961 gatgagcaga actccgccgc cgaccataac ctgccggccg acttggcgcc cttggttcgc
     4021 taatcgacag gtccagcgaa acttccctgc actatgtatt tttttcatgt tttgccagcg
     4081 tcctatatgc tgcttctatg atagcatttc ccctggacgg agagaagcaa gccccggctt
     4141 gagcacctag taggtgctca aatagttaac cgtaatctcg cccgggcggg caaagtcgcc
     4201 gctagcccct gcaataacat ggatctggaa ggtcagcacg tcgcccgctg caatgctaca
     4261 gctaccgaag ctgcttatgg ttcct
//
