LOCUS       DATRMA010000001        77798 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.001, whole genome shotgun sequence.
ACCESSION   DATRMA010000001 DATRMA010000000
VERSION     DATRMA010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 77798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 77798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..77798
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.001"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            complement(48..3146)
                     /locus_tag="VBC28_000001"
     CDS             complement(48..3146)
                     /locus_tag="VBC28_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000089139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEO6814013.1"
                     /translation="MSRMIPGRIRNQGIELYEQGLVSLISQEGNLLKAKVGDCQIEYS
                     LVTEETKCSCDFFARKGYCQHLAALEHFLKNDPEGKAILSKVQVQQESQQETKKKTSF
                     GSVFLDSLIINEDDTIKYQLSAQGEQNPYANDIWWTLKIRRLPDDRSYVIRDIKAFLN
                     TVRKEAYYQIGKQYFETLSLIQFDETSQELIEFLWRLIPSHSSKIDLEFILPNQGRHL
                     SLTRGFFEEGVTLMNALENFSFESDFHQFNHLYFKELEGEDHLYQFKVIVHRQSIELE
                     IKEKDLKPLFANSYLFYRDTFYHLNLKQEKMVTAIRSLPIEGDLAKHIHFDLDDQDKL
                     AAHLLDFKEIGLVDAPRSFSIHDFKVNFEFDINSQNEILLQMVFDYGNDLTVHNRQEL
                     EQLTFASHFKHEEKVFKLLEKYGFAPHFSTSHPAYSAQELYDFYTYMLPQFKKMGTVS
                     LSAKLESYRLIERPQIDIEAKGSLLDISFDFSDLLENDVDQALVALFDNNPYFVNKSG
                     QLVIFDEETKKVSATLQGLRARRAKNGHIELDNIAAFQLSELFANQDNVSFSQHFYQL
                     IEDLRHPEKFKIPGLSVSASLRDYQLTGVRWLSMLDHYGFAGILADDMGLGKTLQTIS
                     FLSTKLTRDSRVLILSPSSLIYNWQDEFHKFAPDVDVAVAYGSKIRRDEIIAERHQVI
                     ITSYSSFRQDFETYSEGNYDYLILDEAQVMKNAQTKIAHSLRSFEVKNCFALSGTPIE
                     NKLLEIWSIFQIILPGLLPGKKEFLKLNPKQVARYIKPFVMRRRKEEVLPELPDLIEM
                     NYPNEMTDSQKVIYLAQLRQIQESIQHSSDADLNRRKIEILSGITRLRQICDTPRLFM
                     DYDGESGKLESLRQLLTQIKENGHRALIFSQFRGMLDIAEREMVAMGLTTYKITGSTP
                     ANERHEMTRAFNAGSKDAFLISLKAGGVGLNLTGADTVVLIDLWWNPAVEMQAISRAH
                     RLGQKENVEVYRLITRGTIEEKILEMQETKKHLVTTVLDGNETHASMSVDDIREILGV
                     SK"
     gene            complement(3301..4611)
                     /gene="der"
                     /locus_tag="VBC28_000002"
     CDS             complement(3301..4611)
                     /gene="der"
                     /locus_tag="VBC28_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEO6814014.1"
                     /translation="MVLPTVAIVGRPNVGKSTLFNRIAGERISIVEDVEGVTRDRIYT
                     TGEWLNRKFSLIDTGGIDDVDAPFMEQIKHQADIAMTEADVIVFVVSGKEGVTDADEY
                     VSRILYKTNKPVILAVNKVDNPEMRNDIYDFYSLGLGDPYPLSSVHGIGTGDILDAIV
                     ENLPVEEENENPDIIRFSLIGRPNVGKSSLINAILGEDRVIASPVAGTTRDAIDTNFV
                     DSQGQEYTMIDTAGMRKSGKVYENTEKYSVMRSMRAIDRSDVVLMVINAEEGIREYDK
                     RIAGFAHETGKGIIIVVNKWDTIEKDNHTVSQWEADIRDNFQFLSYAPIIFVSAETKQ
                     RLHKLPDMIKRISESQNKRIPSAVLNDVIMDAIAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYLRFLENQIREAFVFEGTPINLIARKRK"
     gene            complement(4659..5561)
                     /gene="dnaI"
                     /locus_tag="VBC28_000003"
     CDS             complement(4659..5561)
                     /gene="dnaI"
                     /locus_tag="VBC28_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HEO6814015.1"
                     /translation="MKSVGQALENQGRVPRNTNDELIQMILADAQVAEFIKTHQLSQR
                     EINISMSKFNQFLIERQKFKNKDSQYIAKGYEPILVMNEGYADVSYLETRELIEAQKK
                     QAISDRINLVNLPKSYRNIRMTDFDINNESRMKAMSQLLDFVETYPSYNHKGLYLYGD
                     MGVGKSYLMAAMARELSERKGVSTTLLHFPSFAIDVKNAISSGTVKDEIDAVKSVPIL
                     ILDDIGAEQATSWVRDEILQVILQHRMLEELPTFFTSNYSFNDLERKWANIKGSDETW
                     QAKRVMERVRYLAIEFHLEGPNRR"
     gene            complement(5558..6733)
                     /locus_tag="VBC28_000004"
     CDS             complement(5558..6733)
                     /locus_tag="VBC28_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000074514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HEO6814016.1"
                     /translation="MSPIDEFTYIKQNKIVYDSNSLIQLYFPIMGSDAMALYDYFVHF
                     FDDGIRRHKFSEVLNHLQYGMPRFQDALVMLTALDLLTVYQATGTYLVKLNQAMSNEL
                     FLSNPIYRRLLEKRIGEVAVAELDMKIPKNARDISKKFTDVFSDLGQPKQEVNRSKNV
                     FDLESFKRLMMRDGLRFNNEKDDVLGIYSVSELYHLNWYDTYQLAKQTAINGMIAPQR
                     MKVQQNEGQHIKDNQSFTNNEKVILRESKNDSALVFLEKIKRSRKAVTTSGEKTLLED
                     LAKMNFLDEVINVMVLYTLNKTKSANLNKAYIMKVANDFAFQNVMTAEDAVLKIRDFS
                     DQKVRTKTETKKKQSNVPEWSNPDYKDEVSPEKEIELEQFKTDALKRLERLGKDGES"
     gene            complement(6733..7212)
                     /gene="nrdR"
                     /locus_tag="VBC28_000005"
     CDS             complement(6733..7212)
                     /gene="nrdR"
                     /locus_tag="VBC28_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001203682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HEO6814017.1"
                     /translation="MRCPKCGYNKSSVVDSRQAEEGTTIRRRRECEKCGNRFTTFERL
                     EELPLLVIKKDGTREQFSRDKILNGIIQSAQKRPVSSEDIENCILRIERKIRSEYEDE
                     VSSITIGNLVMDELAELDEITYVRFASVYKSFKDVDEIEELLQQITKRVRSKKSGSV"
     gene            complement(7361..8866)
                     /locus_tag="VBC28_000006"
     CDS             complement(7361..8866)
                     /locus_tag="VBC28_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HEO6814018.1"
                     /translation="MKNKKDQFIGVKQPLSKKLSQLVFILFFSLFTVFSVLVYTSATR
                     YVLHREKINVGRSLEKTRVRLSQANSSLTSDDILEILYNQVFADDIYPHKRQNGIVRT
                     GESIDSILYVNQEMTLYDVNRKPVFSTLRTGMPTIGKSMGKVIISKVADMEGFVGTKA
                     IYSQKTGQLLGYVQIFYNLGRYYSMRQNIIVFLIMMEVLGTVLALVVINSATKRIVRP
                     VKNLHDLMHQISENPSNLEIRSKVRSEDEIGELSRIFDGMLDQLEDYTRRQSQFISDV
                     SHELRTPVAVVKGHIGLLQRWGKDDPEILEESLAAAYHEADRMSLMINDMLNMIRVQG
                     SLELHQDEVTDLSSSISVVIENFRILREDFQFIFENNISDIVWGKIYKIHFEQALMIL
                     IDNAIKYSPSYKEVSVVLSVDNDFATVVVKDKGEGISDEDIEFIFDRFYRTDKSRNRE
                     STQAGLGIGLSVFKQIMDAYHLKVDIKSELNQGTEFIVRIPIKKFEETKEI"
     gene            complement(8856..9545)
                     /locus_tag="VBC28_000007"
     CDS             complement(8856..9545)
                     /locus_tag="VBC28_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO6814019.1"
                     /translation="MGKKILIIEDEKNLARFVSLELLHEGYDVVVETNGREGLDTALE
                     KDFDLILLDLMLPEMDGFEITRRLQAEKTTYIMMMTARDSVMDIVAGLDRGADDYIVK
                     PFAIEELLARVRAIFRRQEIETKTKEKGDSGSFRDLSLNTHNRSAMRGDEEISLTKRE
                     FDLLNVLMTNMNRVMTREELLEHVWKYDVAAETNVVDVYIRYLRGKIDIPGRESYIQT
                     VRGMGYVIREK"
     gene            complement(9780..10313)
                     /locus_tag="VBC28_000008"
     CDS             complement(9780..10313)
                     /locus_tag="VBC28_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000927541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEO6814020.1"
                     /translation="MLLTEIKKSPEGLYFDKKIDIKESLMERHSEIMDISDIQVSGHV
                     VYEDGLYLLDYNMAYDITLPSSRSMKPVVLSEKQTINEVFIEAENVSTKKELVDQELV
                     LILEEDDINLEESVIDNILLNIPLRVLAADEVGVEADLSGKNWSLMTEKQYEEKQAKE
                     KEKSNPFAALEGMFDSD"
     gene            complement(10418..11308)
                     /gene="htpX"
                     /locus_tag="VBC28_000009"
     CDS             complement(10418..11308)
                     /gene="htpX"
                     /locus_tag="VBC28_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000966825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HEO6814021.1"
                     /translation="MLYQQIASNKRKTVVLLIVFFCLLAAIGAAVGYLVLGSYQFGLV
                     LALIIGVIYAVSMIFQSTNVVMSMNNAREVTEDEAPNYFHIVEDMAMIAQIPMPRVFI
                     VEDDSLNAFATGSKPENAAVAATTGLLAVMNREELEGVIGHEVSHIRNYDIRISTIAV
                     ALASAVTLISSIGSRMLFYGGGRRRDDDREDGGNILVLIFSILSLILAPLAASLVQLA
                     ISRQREYLADASSVELTRNPQGMISALEKLDRSEPMGHPVDDASAALYINDPTKKEGL
                     KSLFYTHPPIADRIERLRHM"
     gene            complement(11329..11883)
                     /locus_tag="VBC28_000010"
     CDS             complement(11329..11883)
                     /locus_tag="VBC28_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000537058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEO6814022.1"
                     /translation="MGTMILIAIIALFVIWLIVAYNSLVRSRMHTKESWSQIDVQLKR
                     RNDLIPNLIETVKGYAAYEGKTLEKIAELRAQVAKANTPAEAMTASNELTRQLSSILA
                     VAENYPDLKANNSFVKLQEELTNTENKISYSRQLYNTTTSNYNVKLETFPSNIVGKLF
                     GFKPSQFLETPEEEKEVPKVSFDF"
     gene            12011..12724
                     /gene="rsmG"
                     /locus_tag="VBC28_000011"
     CDS             12011..12724
                     /gene="rsmG"
                     /locus_tag="VBC28_000011"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEO6814023.1"
                     /translation="MTPQAFYQVLIEHGITLTDKQKKQFETYFRLLVEWNEKINLTAI
                     TDKEEVYLKHFYDSIAPILQGYIDNSPLSILDIGAGAGFPSIPMKILYPEIDITIIDS
                     LNKRINFLNILANELELSGVHFFHGRAEDFGQDRVFRAKFDIVTARAVAKMQVLAELT
                     IPFLKVNGRLIALKAAAAEEELISAEKALKTLFSQVTVNKNYKLPNGDDRNITIVSKK
                     KETPNKYPRKAGTPNKKPL"
     gene            12739..14118
                     /locus_tag="VBC28_000012"
     CDS             12739..14118
                     /locus_tag="VBC28_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEO6814024.1"
                     /translation="MKHFFDYKTMSVARKLSISFIAVILLGSILLSLPIFQYANAPKT
                     HYIDHLFTTVSMVCVTGLSVFPISKVYNGWGQIVAILLMQTGGLGLVTLMSLSYYTLR
                     RKMSLNDQTLLQSAITYNSSTDLKKYLYMIFKVTLTLEVLAASILAIDFIPRFGLGHG
                     IFNSIFLAVSAFCNAGFDNLGATSLAQFKLNPLVNIIVCFLIISGGLGFAVWKDLIEA
                     TIQTSHKGPKLIKTFPKRLSNHSKLVLKTTTIILLTGTLLSWLLEFGNFRTIANLSLP
                     KQLMVSFFQTVTMRTAGFSTIDYTQTDFATNLVYIIQMLIGGAPGGTAGGFKVTVIAI
                     LLLLFKAELSGQSQVTFHYRTIPSSIIKQTLSILTFFFIILISGYLLLLELNPHIDPF
                     SLFFEASSALATVGVTMNTTNQLTLGGRIVIMFLMFIGRVGPITVLLSILQKKEKEIH
                     YAETEIILG"
     gene            14131..14802
                     /locus_tag="VBC28_000013"
     CDS             14131..14802
                     /locus_tag="VBC28_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEO6814025.1"
                     /translation="MKTKIIGVLGLGIFGQTLAQELSNFEQDVIAIDSNPENVQAVAE
                     VVTKAAIGDITDLAFLKHIGISDCDTVIIATGNSLESSVLAVMHCKKLGVPQVIAKAR
                     NLVYEEVLYEIGADLVISPERESGQNVAANLMRNKITDVFQIESDISVIEFKIPKSWV
                     GKTVEQLNIRHKFDLNLIGIRKAKNKPVDTEVPINSPLEEGIILVAIANSDAFQRYDY
                     LGYFN"
     gene            complement(14837..15667)
                     /locus_tag="VBC28_000014"
     CDS             complement(14837..15667)
                     /locus_tag="VBC28_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEO6814026.1"
                     /translation="MAKQLIGYQEGYGFLYRLSGASKLLFFILVSVACMTTYDTRFLI
                     VVSLGSLVLFRLAKIKWQQVSFVMTLVVVFAVLNIIMVYLFAPHYGDKIYGSSSLLLK
                     GIGPYDVTSQELFYLFNLILKYFCTVPLALLFLMTTNPSQFASSLNQLGLSYKIAYAV
                     SLTLRYIPDVQEEFYTIRRAQEARGIELSKKSNLVARIKGNLQIVTPLIFSSLERIDT
                     VATAMELRRFGKNKRRTWYSKQSLEKSDIVLIILALASLFVSLYLIHLNHGRFYNPWQ
                     "
     gene            complement(15660..17336)
                     /locus_tag="VBC28_000015"
     CDS             complement(15660..17336)
                     /locus_tag="VBC28_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6814027.1"
                     /translation="MKDFIEWKDFTFQYDVQSEPTLKGINLSIPKGEKVLILGPSGSG
                     KSTLGHCLNGIIPNTHKGQYSGIFTINHKNAFDLSIYDKSHLVSTVLQDPDGQFIGLT
                     VAEDIAFALENDVVAQEEMASIVEMWAKRLEIAPLLSKRPQDLSGGQKQRVSLAGVLV
                     DDSPILLFDEPLANLDPQSGQDIMALVDRIHREQDATTIIIEHRLEDVFYERVDRVVL
                     FSDGQIIYNGEPDQLLKTNFLSEYGIREPLYISALKNLGYDFEKQNTMTSIDDFDFSE
                     LLIPKMRALDLDKHTDKLLSVQHLSVSYDLENNTLDDVSFDLYKGQRLAIVGKNGAGK
                     STLAKALCQFIPNNATLIYNNEDVSQDSIKERAERIGYVLQNPNQMISQAMVFDEVAL
                     GLRLRGFSDNDIESRVYDILKVCGLYQFRNWPISALSFGQKKRVTIASILILNPEVII
                     LDEPTAGQDMKHYTEMMSFLDKLSCDGHTIVMITHDMQLMLEYTDRAIVIDNGLIVAD
                     DHPINILSNTELLKKTHLKKTSLFALADRLGISPQKLTQWYIDNQGGKNG"
     gene            complement(17353..17901)
                     /locus_tag="VBC28_000016"
     CDS             complement(17353..17901)
                     /locus_tag="VBC28_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type riboflavin transporter
                     substrate-binding protein"
                     /protein_id="HEO6814028.1"
                     /translation="MNTNTIKKVVATGIGAALFIIIGMLVNIPTPIPNTNIQLQYAVL
                     ALFAVIYGPGVGFFTGFIGHALKDSIQYGSPWWTWVLVSGLLGLMIGFFAKKLAIQLS
                     GMTKKDLLLFNVVQVIANLIGWSVVAPYGDIFFYSEPASKVFAQGFLSSLVNSITIGV
                     GGTLLLLAYAKSRPQKGSLSKD"
     gene            complement(18070..19278)
                     /gene="sstT"
                     /locus_tag="VBC28_000017"
     CDS             complement(18070..19278)
                     /gene="sstT"
                     /locus_tag="VBC28_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HEO6814029.1"
                     /translation="MKRFLKAWRKTSLIKKITIGVVIGLFLGILVPKASAIGLLGQLF
                     VGGLKAIAPLLVFTLVISALSQHREGGKTNMSTIIGLYITATFAAALIAVVVNYIFPL
                     TLILKTPAKTDLLPPKGISEVFQSLLLKIVDNPIHAITEANYMSILFWAVIFGLAMRS
                     SNQRTKDLMQTFADATSQVVKWIINLAPIGIMGLVFTSISENGIGILGDYGLLILVLV
                     GTMLFVALVVNPIIAFVMMRKNPYPLVLRCLKDSGITAFFTRSSAANIPVNMRLCEDL
                     GLDKDTYSVSIPLGAAINMAGAAITINILTLAAVNTLGITVDFPTAFLLSVVAAVSAC
                     GASGVTGGSLLLIPVACSLFGISNDVAMQVVGVGFIVGVIQDSCETALNSSTDVLFTA
                     VAEKSVFGKK"
     gene            19454..20821
                     /gene="brnQ"
                     /locus_tag="VBC28_000018"
     CDS             19454..20821
                     /gene="brnQ"
                     /locus_tag="VBC28_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HEO6814030.1"
                     /translation="MKLKNTYIIIGLMLFALFFGAGNLIYPAYLGIYSGSNWAWAIIG
                     FCLTGVTLPLLGVAAVAYSGATDVESFTKPVSKTYAIFFAVALYLSIGPFFAIPRTGA
                     TSFSVGIQPILGDSSLVKIIYGLIFFGISYFLAIRPSQIVDRIGKYLTPFLLTVISIL
                     IIASLIHPAAIVGTPHNASTNVSDAFKDLPFLAGLIQGYGTMDALASIAFAIIVIQAS
                     KQYGAITKKEITSMALKSGAIATFLLAFIYIFVGRIGATSQSLFKFANGSFLLHNTPI
                     DGGHVLSQSANFYLGIVGQAILGTAIFLACLTTATGLITACAEYFHKLLPKISHITWA
                     TIFTLIAITFYFGGLSEIIRWSLPVLYLLYPLTIVLIFLVFFDQKFESSRIVYQTSIA
                     ATAVAALYDALSKLGEMTGLFTIPSALTTFFTKVVPLGEYSMGWISFAICGVLVGLIL
                     KKVKA"
     gene            20935..21990
                     /locus_tag="VBC28_000019"
     CDS             20935..21990
                     /locus_tag="VBC28_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HEO6814031.1"
                     /translation="MRAATYLSANELSLTDKAKPQVIKPTDAVVRLVKTTICGTDLHI
                     LGGDVPACKPGTTLGHEGIGIVEEVGSAVNHFKVGDKVIISCVTACNTCYYCKHQLPS
                     HCEDGGWILGHLIEGTQAEYVHIPHADGSLYHAPEGVCDDALVMLSDILPTSYEIGVL
                     PSHIKPGDTVCIVGAGPIGLSALLTAQFYSPAKIIMVDLSQKRLEASKKFGATHTILS
                     TSTQEVKEEIDKITKGRGVDVVLECVGYPATFDICQNVVSIGGHIANVGVHGKPVEFN
                     LQDLWIKNITLNTGLVNANTTEMLLEVLETGKIDATQLVTHHFKLSEIEEAYKVFKAA
                     EENNTLKVIIENDITPA"
     gene            complement(22039..22731)
                     /locus_tag="VBC28_000020"
     CDS             complement(22039..22731)
                     /locus_tag="VBC28_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000574038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6814032.1"
                     /translation="MIEWIQTHLPNVYQMGWEGAYGWQTAIVQTLYMTFWSFLIGGLM
                     GLLGGLFLVLTSPRGVIANKLVFGVLDKVVSVFRALPFIILLALIAPVTRVIVGTTLG
                     SPAALVPLSLAVFPFFARQVQVVLAELDGGVIEAAQASGGTLWDIIVVYLREGLPDLI
                     RVSTVTLISLVGETAMAGAIGAGGLGSVAITKGYNYSRDDITLVATILILLLIFFIQF
                     LGDFLTRRLSHK"
     gene            complement(22728..23798)
                     /locus_tag="VBC28_000021"
     CDS             complement(22728..23798)
                     /locus_tag="VBC28_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HEO6814033.1"
                     /translation="MSKEPIIKLDNIDVTFHQKKREINAVKDVTIHINQGDIYGIVGY
                     SGAGKSTLVRVINLLQEPSAGKITIDDQVIYDNKVTLTSTQLREQRREIGMIFQHFNL
                     MSQLTAEQNVAFALKHSGLSKEAKAAKVAKLLELVGLSDRAQNYPSQLSGGQKQRVAI
                     ARALANDPKILISDESTSALDPKTTKQILALLQDLNKKLGLTIVLITHEMQIVKDIAN
                     RVAVMQNGKLIEEGSVLDIFSHPRESLTQDFIKIATGIDEAMLKIEQQEVVKNLPVGS
                     KLVQLKYAGHSTDEPLLNQIYKEFEVTANILYGNIEILDGIPVGEMVVILSGDEEKLR
                     QACQAITDSQVQLTLLKEGGKA"
     gene            complement(23791..25167)
                     /locus_tag="VBC28_000022"
     CDS             complement(23791..25167)
                     /locus_tag="VBC28_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000176659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEO6814034.1"
                     /translation="MTLTNEENQIQKFLSDTIVQDDINILRDLIAIKSIFAQKVGLND
                     LSSYLGEVFIKAGAEVIIDDSYSAPFIVANFKSSKVDAKRIIFYNHYDTVPADEVEQW
                     TEDPFTLSLRYGKMYGRGVDDDKGHITARLSAVKKYLSRHKGELPLDITFIVEGAEES
                     ASVGLDYYLEKYQEQLQGADLIVWEDGPKNPKGQLEIAGGNKGIVTFDLSVSSADVDI
                     HSSFGGVVDSSTWYLIQALNTLRDNKGHILVEGIYDKVIPPTKRELELVEKYSYRSAK
                     ALEGAYQLVLPSLADSHKTFLRKLYFEPSIAIEGITSGYQGEGVKTILPAYAKCKAEV
                     RLVPGLTPKGVLDSIQNHLKENGFKDIELTYTLGEMSYRSDMSAPSILKVVDLAEQFY
                     PEGISLLPTSPGTGPMYLVHQALRAPIAAIGIGHANSRDHGVDENVSIADYYTHIELV
                     EALIESYE"
     gene            complement(25301..26125)
                     /locus_tag="VBC28_000023"
     CDS             complement(25301..26125)
                     /locus_tag="VBC28_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001931032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEO6814035.1"
                     /translation="MKLKHIVLGLALTTLLGVTFSNQEVSASSTSSKVVKVGVMTFSD
                     TEKARWDKIEKLVGDKAKIKFTEFTDYTQPNQATANKDVDINAFQHYNFLENWNKENK
                     KNLIPLEKTYLAPIRIYSEKVKSLKKLKKGATIAIPNDATNGSRALYVLQSAGLIKLN
                     VSGKKVATVANITSNKKDINIQELDASQTPRALKDVDAAIINNTYIEQANLKPSDAIF
                     VEKSDKNSKQWINIIAGRKNWKKQKNAKAIQAILDAYHTDEVKKVIKDTSADIPQW"
     gene            complement(26266..27099)
                     /locus_tag="VBC28_000024"
     CDS             complement(26266..27099)
                     /locus_tag="VBC28_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEO6814036.1"
                     /translation="MTIKKVLSVTGIILVTVASLAACSSKSHTTKTGKKEVNFATVGT
                     TAPFSYVKDGKLTGFDIEVAKAVFKGSDNYKVTFKKTEWSSVFTGIDSGKFQMGGNNI
                     SYSSERSQKYLFSYPIGSTPSVLAVPKNSNIKAYNDISGHKTQVVQGTTTAKQLENFN
                     KEHQKNPVTLKYTNENITQILTNLSDGKADFKLFDGPTVNAIIKNQGLTNLKTIPLTM
                     RDQPYIYFIFGQDQKDLQKYVNNRLKQLRKDGTLSKIAKEYLGGDYVPNEKDLVTPKE
                     K"
     gene            complement(27201..27890)
                     /locus_tag="VBC28_000025"
     CDS             complement(27201..27890)
                     /locus_tag="VBC28_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEO6814037.1"
                     /translation="MLTKPIIGITGNEREMSDIPGYYYDSVSRHISEGVKNAGGLPVI
                     LPISEAESAKAYVEMIDKLIISGGQNVLPSYYGEEKIIESDDYSLARDIFEFALVEEA
                     LKQNKPIFAICRGMQLVNVALGGTLNQSIDNHYQEPYIGFAHYLNVEKGSFLEGFISG
                     DFKINSLHRQSVKLLAEGLIVSARDPRDGTVEAYESRTEQCIIGVQWHPELMLHQIEN
                     QTLFGYFVNET"
     gene            complement(28045..28761)
                     /locus_tag="VBC28_000026"
     CDS             complement(28045..28761)
                     /locus_tag="VBC28_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008088325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEO6814038.1"
                     /translation="MGRKWANIVAKKTAKDGANSKVYAKFGVEIYVAAKQGEPDPESN
                     SALKFVLDRAKQAQVPKHVIDKAIDKAKGNTDETFVEGRYEGFGPNGSMIIVDTLTSN
                     VNRTAANVRTAYGKNGGNMGASGSVSYLFDKKGVIVFAGDDADTVFEQLLEADVDVDD
                     VEAEEGTITVYTAPTDLHKGIQALRDNGVEEFQVTELEMIPQSEVVLEGDDLETFEKL
                     IDALESDDDVQKVYHNVADF"
     gene            complement(28879..29865)
                     /gene="dhaQ"
                     /locus_tag="VBC28_000027"
     CDS             complement(28879..29865)
                     /gene="dhaQ"
                     /locus_tag="VBC28_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DhaKLM operon coactivator DhaQ"
                     /protein_id="HEO6814039.1"
                     /translation="MRKTEHHKMEYVFETIKAITKTNPKVEQIKDLPAIHYLGQSEQV
                     IPILSGGGSGHEPAHFGYVGEGMLSAAISGPIFVPPCASDILETIRFINRGKGVFVII
                     KNFEADLEEFSQAIEQARQEGIPIKYIVSHDDISVETSNFKIRHRGVAGTVLLHKIIG
                     QAALEGASLDELEQLGLSLTTSMATLGVASKSATILGQHQPVFDLEEGYISFGIGIHG
                     EPGYRTMPFVSMEHLANELVNKLKMKLRWQDGEAFILLINNLGGSSKMEELLFTNAVM
                     EFLALDDLQLPFIKTGHLITSLDMAGLSVTLCRVKDSRWIDYLKHKTDARAW"
     gene            complement(29875..30411)
                     /gene="dhaS"
                     /locus_tag="VBC28_000028"
     CDS             complement(29875..30411)
                     /gene="dhaS"
                     /locus_tag="VBC28_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase transcriptional
                     activator DhaS"
                     /protein_id="HEO6814040.1"
                     /translation="MTSSLITKKKIAKSFKRLFISQAFDKISVSDIMEDAGIRRQTFY
                     NHFVDKYALLEWIFQTELSEQVTDNLDYISGFQLLSELLTFFKMNQEFYIKLFQIEDQ
                     NDFSSYFESYCEQLVDKLLSDYSKSNFNQKERVTFINYHSKALSYFIKYELINNSKEE
                     LFNRKVIEKIIRTSIENY"
     gene            30585..31574
                     /gene="dhaK"
                     /locus_tag="VBC28_000029"
     CDS             30585..31574
                     /gene="dhaK"
                     /locus_tag="VBC28_000029"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HEO6814041.1"
                     /translation="MKKILNQPTDVVTEMLDGLAYVHNDLVHRIEGFDIIARNEEKSG
                     KVALISGGGSGHEPSHAGFVGEGMLSAAVCGAVFTSPTPDQVLEAIKEADEGAGVFMV
                     IKNYSGDIMNFEMAQDMAEMEGIEVASVVVDDDIAVEDSLYTQGKRGVAGTILVHKIL
                     GHAARHGKSLQEIKAIADELVPNIHTVGLALSGATVPEVGKPGFVLAEDEIEFGIGIH
                     GEPGYRKEKMQPSKALATELVDKLIESFDAKSGEKYGVLINGMGATPLMEQYVFANDV
                     AKLLEDKGIEVNYKKLGNYMTSIDMAGLSLTLIKLENQEWLEALNSDVTTIAW"
     gene            31612..32190
                     /gene="dhaL"
                     /locus_tag="VBC28_000030"
     CDS             31612..32190
                     /gene="dhaL"
                     /locus_tag="VBC28_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HEO6814042.1"
                     /translation="MEVKTAIEWMHTFNQKIQSNKDYLSELDTPIGDGDHGGNMARGM
                     TAVIENLDNNEFSSAADVFKTVSMQLLSKVGGASGPLYGSAFMGITKAEQSKSTISEA
                     LGAGLEMIQKRGKAELNEKTMVDVWHGVIEAIEKNELTEDRIDSLVDATKGMKATKGR
                     ASYVGERSVGHIDPGSFSSGLLFKALLEVGGV"
     gene            32190..32564
                     /locus_tag="VBC28_000031"
     CDS             32190..32564
                     /locus_tag="VBC28_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002960428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HEO6814043.1"
                     /translation="MSDIGIIVVSHSKNIAQGVVDLISEVAKDVSITYVGGTEDGEIG
                     TSFDQVQQIVEQNDKKTLLAFFDLGSAKMNLELVADFSEKNIIINSVPVVEGAYTAAA
                     LLQAGADLDSIQSQLAELTINK"
     gene            32574..33287
                     /locus_tag="VBC28_000032"
     CDS             32574..33287
                     /locus_tag="VBC28_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEO6814044.1"
                     /translation="MTQFLGEFLGTFILVLLGDGVVAGNVLSKTKEEGTGWTAIVFGW
                     GIACTVAVYVSGLFSPAHLNPAVTLAMASIGAISWGQVIPFIIAQMLGAMVAATILWL
                     HYYPHWKETKDSGLILASFSTGPAIRHTPSNLLGEIIGTAILVITIMAIGPSKVAAGL
                     GPIIVGIVIFAVGFSLGPTTGYAINPARDLGPRLMHAILPIENKGNSDWSYAWIPVVG
                     PIIGGVLGAILYNIALTLV"
     gene            complement(33552..33956)
                     /locus_tag="VBC28_000033"
     CDS             complement(33552..33956)
                     /locus_tag="VBC28_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000675692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEO6814045.1"
                     /translation="MKGFHGNIEKLTLGNTNFRQVLYTAEHCQLVLMTLPVGGEIGSE
                     IHAENDQFFRFEAGHGKVVIDGNEYEVADGDAIIVPAGAEHNVINTSETEMLKLYTIY
                     SPAHHKDGIIRATREEAEENEEDFDGVTTETK"
     gene            complement(34095..34808)
                     /locus_tag="VBC28_000034"
     CDS             complement(34095..34808)
                     /locus_tag="VBC28_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEO6814046.1"
                     /translation="MKTVKEISHISGISVRTLHYYDEIDLLSPSFVGENGYRYYDDES
                     LIKLQEILLFKELEFPLKKIKEIMDSPNYDRNQALLDQIRWLELKKQRLEEVIEHAKS
                     IQRGKNMSDFTAYNQEELEAFQEEARTRWGDTDSYKEFENSHSKNDFSMISQAMSQIF
                     KDFGQLKELSPTDEKVQKQVQILQDYITAQFYNCTNDLLASLGIMYIQDERFQKSIDN
                     WGGQGTALFVSKAIDSYCH"
     gene            complement(34901..36010)
                     /locus_tag="VBC28_000035"
     CDS             complement(34901..36010)
                     /locus_tag="VBC28_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HEO6814047.1"
                     /translation="MTVTGIVAEFNPFHNGHKYLLEQAQGIKVIAMSGNFMQRGEPAI
                     VDKWTRSQMALENGADLVIELPFLVSVQSADYFASGAVSILARLGVDNLCFGTEEMLD
                     YARIGDIYVNKKEEMEAFLKKQSDSLSYPQKMQAMWQEFAGITFSGQTPNHILGLAYT
                     KAASQNGIRLNPIQRQGAGYHSSEKTEIFASATSLRKHQSDRFFVEKGMPNSDLFLNS
                     PQVVWQDYFSLLKYQIMTHSDLTQIYQVNEEIANRIKSQIRYVETVDELVDKVATKRY
                     TKARIRRLLTYILINAVESPIPNAIHVLGFTQKGQQHLKSVKKSVDIVTRIGSQTWDS
                     LTQRADSVYQMGNANIAEQTWGRIPFYQPVSQSDL"
     gene            complement(36007..36258)
                     /locus_tag="VBC28_000036"
     CDS             complement(36007..36258)
                     /locus_tag="VBC28_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000583227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6814048.1"
                     /translation="MIIFLYACLMIFGILYIIERRVSQKKSKVILELLMNPKLHLDLL
                     SALKPFEKETDRLIYLRKTYHLDIIDAVTLNNQLKEKEV"
     gene            complement(36281..37015)
                     /locus_tag="VBC28_000037"
     CDS             complement(36281..37015)
                     /locus_tag="VBC28_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEO6814049.1"
                     /translation="MATYETFAAVYDAVMDDTLYAKWTDFSLRHFPKGKKKLLELACG
                     TGIQSVRFAQAGYAVTGLDLSGDMLKLAKKRATSAHQSIQFIEGNMLDLSNVGKYDLI
                     TCYSDSICYMQDEVEVGDVFIEVYKALEENGVFIFDVHSTYQTDKVFPGYSYHENADD
                     FAMVWDTYEDDAPHSIVHELTFFVQEEDGRFTRHDEVHEERTYDILTYDILLEQAGFK
                     DVKVYADFEDKKPTATSARWFFVAHK"
     gene            complement(37081..37437)
                     /gene="rsfS"
                     /locus_tag="VBC28_000038"
     CDS             complement(37081..37437)
                     /gene="rsfS"
                     /locus_tag="VBC28_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEO6814050.1"
                     /translation="MTEKDLLQLVVKAADEKRAEDIVILDLQPVTSVADYFVIMSASN
                     SRQLEAIADNIREQVKGNGGDASHLEGDSKAGWVLLDLNSVVVHIFSEDERQHYNLEK
                     LWHEAPLLDAEVFMTE"
     gene            complement(37439..37960)
                     /locus_tag="VBC28_000039"
     CDS             complement(37439..37960)
                     /locus_tag="VBC28_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000128172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEO6814051.1"
                     /translation="MTCALLLIDIQQGIMDKKPKHLTNFAVLLDDLLLSAKGSNCEVI
                     WIRHHDKELPQGSPQWEIWEQRHLVTHHKIIDKTYNSCFKDTHLHDYLQSKHISQLIM
                     MGLQTEYCFDTSVKVAFEYGYDIFIPQGGHLTFDTPTLSGDSIKKHYENIWHHRFATM
                     VAKDSLLKLINEE"
     gene            complement(38023..38610)
                     /gene="yqeK"
                     /locus_tag="VBC28_000040"
     CDS             complement(38023..38610)
                     /gene="yqeK"
                     /locus_tag="VBC28_000040"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HEO6814052.1"
                     /translation="MTYKDYTGLDRTELLSKVRHMMSDKRFNHVLGVERAAIELAERY
                     GYDKEKAGLAALLHDYAKELSDDEFLRLIDKYQPDPDLKKWGNNIWHGLVGIYKIQED
                     LAIKDQDILAAIAKHTVGSAQMSTLDKIVYVADYIEHNRDFPGVEEARELAKVDLNKA
                     VAYETARTVAFLASKAQPIYPKTIETYNAYIPYLD"
     gene            complement(38607..39239)
                     /locus_tag="VBC28_000041"
     CDS             complement(38607..39239)
                     /locus_tag="VBC28_000041"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEO6814053.1"
                     /translation="MALELLTPFTKVELEEKKRDTNRKQIGIMGGNFNPVHNAHLVVA
                     DQVRQQLCLDQVLLMPEFQPPHIDKKETIDEQHRLKMLELAIEGIDGLSIEPIEIERK
                     GISYTYDTMKLLIEKNPDVDYYFIIGADMVEYLPKWHRIDELVKMVQFVGVQRPKYKA
                     GTSYPVIWVDLPLMDISSSMIRQFIKSNRQPNYLLPREVLDYIRKEGLYK"
     gene            complement(39369..39686)
                     /gene="yhbY"
                     /locus_tag="VBC28_000042"
     CDS             complement(39369..39686)
                     /gene="yhbY"
                     /locus_tag="VBC28_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HEO6814054.1"
                     /translation="MLTSKQRAFLKSEAHSMKPIIQIGKNGLNDQIKTSVRNALDARE
                     LIKVTLLQNTDEDIHDVAEVLEDEIGCDTVLKIGRILILYKESARKENRKISVKVKAV
                     KSN"
     gene            complement(39779..40897)
                     /gene="yqeH"
                     /locus_tag="VBC28_000043"
     CDS             complement(39779..40897)
                     /gene="yqeH"
                     /locus_tag="VBC28_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HEO6814055.1"
                     /translation="MEELFCIGCGARIQTENKDAAGYTPRAALEKGLETGELYCQRCF
                     RLRHYNEITDVHITDDEFLKLLHEVGDSDALVVNVIDIFDFNGSIIPGLSRFVAGNDV
                     LLVGNKKDILPKSVKDGKVTQWLTERAHEEGLRPVDVILTSAQNHHAIKDLIDTIEKY
                     RHGQDVYVVGVTNVGKSTLINAIIREITGSRDVITTSRFPGTTLDKIEIPLDDGSYIF
                     DTPGIIHRHQMAHYLTAKNLKYVSPKKEIKPKTYQLNSEQTLFLAGLARFDFISGQKQ
                     GFTAYFDNNLNLHRTKLVGADEFYTKHVGKLLTPPTGKEVSDFPKLVRHEFTIKDKMD
                     IVYSGLGWIRVKSEAENPVVVAAWAPEGVAVVLRKALI"
     gene            complement(40897..41457)
                     /locus_tag="VBC28_000044"
     CDS             complement(40897..41457)
                     /locus_tag="VBC28_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HEO6814056.1"
                     /translation="MNSKIYFLEMKLSIDDYRPTFVVEAVYDLRAEDLLRHGIRAVLV
                     DLDNTLIAWNNPDGTAEVRAWLDEMTTADISVVVVSNNNHARVERAVSRFGVDFVSRA
                     MKPFTRGINMAIERYGFDRDEVIMVGDQLMTDIRASHRAGIKSVLVKPIVKSDAWNTK
                     FNRLRERRVWKKIEENYGKIVYQKGI"
     gene            complement(41541..42455)
                     /locus_tag="VBC28_000045"
     CDS             complement(41541..42455)
                     /locus_tag="VBC28_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEO6814057.1"
                     /translation="MTKKEKGTMMTLAAGLAWGISGISGQYLMSHGVHVNLLTSLRLL
                     ITGIFLLSLARSKQKEHLVAAWKQPKFLKQVLLFSIFGLVLNQYAFLRAIHLTNAGTA
                     TVLQYMAPILILSIVCILNRQRPTSFEIIAIAMAILGTYMIATHGKLGSLAITPKGLM
                     WGLGSAITYSIYILLPVKLIHEWGSTIVIGSGMFIGGILFSLVTKAWQYPLQINVMSI
                     LAYIGIIGIGTIFAYTFFLKGVSIVGAVKGSLLASVEPVSSVFLTVLVLGEIFYPIDL
                     LGMLFIFLAVTLISYKDLIALKKMKRVR"
     gene            complement(42585..44027)
                     /gene="gatB"
                     /locus_tag="VBC28_000046"
     CDS             complement(42585..44027)
                     /gene="gatB"
                     /locus_tag="VBC28_000046"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEO6814058.1"
                     /translation="MNFETVIGLEVHVELNTNSKIFSPSSAHFGQEQNANTNVIDWSF
                     PGVLPVMNKGVIDAGIKAALALNMDIHQNMHFDRKNYFYPDNPKAYQISQFDEPIGYN
                     GWIEIELEDGTRKKIRIERAHLEEDAGKNTHGTDGYSYVDLNRQGVPLIEIVSEADMR
                     SPEEAYAYLTALKEIIQYTGISDVKMEEGSMRVDANISLRPYGQEEFGTKAELKNLNS
                     FNNVRKGLIHEEKRQAQVLRSGGQIQQETRRFDETTGETILMRVKEGSSDYRYFPEPD
                     LPLFDISDEWIDQVRLELPEFPQERRAKYVSSFGLSSYDASQLTATKATSDFFEKAVA
                     IGGDAKQVSNWLQGEVAQFLNSESKSIEEIGLTPENLVEMIGLIADGTISSKIAKKVF
                     VHLAKNGGSAEEFVKKAGLVQISDPEVLIPIIHQVFADNEAAVIDFKSGKRNADKAFT
                     GYLMKATKGQANPQVALKLLAQELAKLKEE"
     gene            complement(44027..45493)
                     /gene="gatA"
                     /locus_tag="VBC28_000047"
     CDS             complement(44027..45493)
                     /gene="gatA"
                     /locus_tag="VBC28_000047"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEO6814059.1"
                     /translation="MSFNNQSIDQLHDFLVKKEISATELTKATLEDIHAREQAVGSFI
                     TISDEMAIAQAKEIDDKGIDADNVMSGIPLAVKDNISTKGILTTAASKMLYNYEPIFD
                     ATAVEKLYAKDMIVIGKANMDEFAMGGSTETSYFKKTNNAWDHSKVPGGSSGGSAAAV
                     ASGQVRLSLGSDTGGSIRQPASFNGIVGMKPTYGRVSRFGLFAFGSSLDQIGPMSQTV
                     KENAQLLTVISGHDVRDSTSSERTVGDFTAKIGQDIQGMKIALPKEYLGEGIAQGVKE
                     TIIKAAKHLEKLGAVIEEVSLPHSKYGVAVYYIVASSEASSNLQRFDGIRYGYRTENY
                     KNLDDIYVNTRSEGFGDEVKRRIMLGTFSLSSGYYDAYYKKAGQVRSLIIQDFEKVFA
                     DYDLILGPTAPTTAFDLDSLNHDPVAMYLADILTIPVNLAGLPGISIPAGFDQGLPVG
                     MQLIGPKFSEETIYQVAAAFEATTDYHKQQPKIFGGEN"
     gene            complement(45493..45795)
                     /gene="gatC"
                     /locus_tag="VBC28_000048"
     CDS             complement(45493..45795)
                     /gene="gatC"
                     /locus_tag="VBC28_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEO6814060.1"
                     /translation="MKISEEEVRHVANLSKLRFSDQETKEFASSLSKIVDMIELLNEV
                     DTEGVPVTTTMADRKTVMREDIAQPGHNRDDLFKNVPQHQDYYIKVPAILEDGGDA"
     gene            complement(45933..48578)
                     /locus_tag="VBC28_000049"
     CDS             complement(45933..48578)
                     /locus_tag="VBC28_000049"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HEO6814061.1"
                     /translation="METKFVYHFDEGCKEMKELLGGKGANLAEMTSIGLPVPQGFTIT
                     TQACNDYYDNACHIRESILSQIDQAMAQLEVEQNKQLGSVDDPLLVSVRSGSVFSMPG
                     MMDTVLNLGLNDRSVQGLVKKTEDERFAYDSYRRFIQMFADVVTGIPKYKFDTILDRL
                     KTDKCYQDDTELTGSDLKRLVEFYKELYQKEAGEKFPQDPKRQLLLAIEAVFKSWNNP
                     RAKIYRKLNDIPETLGTAVNIQAMVFGNMGNNSGTGVAFTRNPSTGAANLFGEYLINA
                     QGEDVVAGIRTPQSISKLAEQMPIIYQEFVSVTQKLEAHYRDMQDMEFTIENGNLYML
                     QTRSGKRTAKAAIKIAVDQVNEGLISKEEAILRIEPKQLDQLLHPSFDLKSLKKAIIL
                     TTGLPASPGAAYGKVYFHAEDVVKEMKKGNPVLLVRQETSPEDIEGMVSANGIITARG
                     GMTSHAAVVARGMGKPCVAGCSQLLVDEVRREISIGHQTIKEGEMLSIDGATGNVYIG
                     QVPMAETSVDRDFEIFMKWVDENRDMMVCSNADNPRDAQKALDFGAEGIGLCRTEHMF
                     FDDERIPVVREMILADEILSRRKALERLLSFQRDDFYQIFKVLKGKACTIRLLDPPLH
                     EFLPHDKESIESMARQMGISTLAIEKRIQTLEEFNPMLGHRGCRLAITYPEIYQMQVR
                     ALVQGAILAMKEGYEAKPEIMIPLVTAHEEISIIRDLIEETIVEESKSKKINLSFPIG
                     TMIETPRACMIADDIAKFADFFSFGTNDLTQMSFGFSRDDAGKFLGEYVDKGLLKKDP
                     FQVLDQKGIGRFIGQAVRLGKEVKPNLKIGICGEHGGEPSSIEFCYQLGLHYVSCSPF
                     RIPIAKLAAAQAKIKQSRNDITIDK"
     gene            complement(48591..49421)
                     /locus_tag="VBC28_000050"
     CDS             complement(48591..49421)
                     /locus_tag="VBC28_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HEO6814062.1"
                     /translation="MEDQLTIFIISDSLGETAKAIAKACLSQFPGHDDWHFQRFSYIN
                     SQERLEQVFEEASQKTVFMMFSLVDVALASYAQKRCESEHYAYVDLLTNVIQGISRIS
                     GIDPLGEPGILRRLDNDYFKRVESIEFAVKYDDGRDPRGILQADLVIIGISRTSKTPL
                     SMFLADKNIKVINIPLVPEVPVPKELRMIDSRRIIGLTNSVDHLNQVRKVRLKSLGLS
                     STANYASLERILEETRYAEEVMKNLGCPIINVSDKAIEETATIILEILKTNGQVAKNL
                     "
     gene            complement(49432..50061)
                     /locus_tag="VBC28_000051"
     CDS             complement(49432..50061)
                     /locus_tag="VBC28_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011074751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO6814063.1"
                     /translation="MQLNQRQERIIDIIKQFQPITGELIAEKLGVTRAALRSDLAVLS
                     MLGIIDAKPKVGYFYLGQYHASIGTSHFEKMTVSEIMGIPLTVHQKDSVYDVIVHIFM
                     EDAGCAFILDDDDFLCGVVSRKDLLKISIGGGDLSKMPIGMVMTRMPHVTTVLENESL
                     FAAADKLVSRKVDSLPVVRHDKQYPEKFKVIGKLSKTILASLFLEIRDY"
     gene            50250..51215
                     /locus_tag="VBC28_000052"
     CDS             50250..51215
                     /locus_tag="VBC28_000052"
                     /EC_number="1.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HEO6814064.1"
                     /translation="MTIKNLTVAGSGVLGSQIAFQAAYKGMSVTIYDINDEALNKGKE
                     RIKKLAKVYQSEIETAKEAYSDKAKSIKYNKNLLPSLDHIFLSKVADSLDLIADLPNQ
                     ITFSKNLDQAVSDADLVIEAVPETVSIKEDFYKQLAKVAPSKTIFATNSSTLVPSQFA
                     DITGRPDKFLAMHFANNIWQNNIVEIMGHKGTDDEVIKEALAFSKDIGMVPLHIHKEQ
                     PGYILNSILVPFLESALALYYDKVSDSETIDKTWKLGTGAPMGPLEILDIIGIDTAYN
                     IMKNYSDTNSDPNSLHAHLAKMLKEEFIDKGRTGKAAGHGFYDYD"
     gene            complement(51262..51810)
                     /locus_tag="VBC28_000053"
     CDS             complement(51262..51810)
                     /locus_tag="VBC28_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEO6814065.1"
                     /translation="MTKALISIDYTYDFVADDGKLTAGKPAQSIASAIADVTEKAYRS
                     GDYIFFAIDNHDIGDVFHPESNLFPEHNIKGTSGRNLYGPLGTLYETIKEDSRVFWID
                     KRHYSAFSGTDLDIRLRERRVDTLILTGVLTDICVLHTAIDAYNLGYKIEVPAAAVAS
                     LNDSNHQWALNHFKTVLGATIL"
     gene            complement(51877..52662)
                     /gene="codY"
                     /locus_tag="VBC28_000054"
     CDS             complement(51877..52662)
                     /gene="codY"
                     /locus_tag="VBC28_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001133183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HEO6814066.1"
                     /translation="MPNLLEKTRKITSILQRSVDSLDAELPYNTMAAQLADIIDCNAC
                     IINGGGNLLGYAMKYKTNTDRVEEFFETKQFPDYYVKSASRVYDTEANLSVDNDLSIF
                     PVETKENFQDGITTIAPIYGGGMRLGTFIIWRNDKEFSDDDLILVEIASTVVGIQLLN
                     LQTENLEENIRKQTAVTMAINTLSYSEMKAVAAILGELDGLEGRLTASVIADRIGITR
                     SVIVNALRKLESAGIIESRSLGMKGTYLKVINEGIFDKLKEYN"
     gene            complement(52788..53999)
                     /locus_tag="VBC28_000055"
     CDS             complement(52788..53999)
                     /locus_tag="VBC28_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000688111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEO6814067.1"
                     /translation="MKIFDKSMKLEHVAYDIRGPVLEEADRMRANGEKILRLNTGNPA
                     AFGFEAPDEVIRDLITNARESEGYSDSKGIFSARKAVMQYYQLQNIHVDMDDIYIVNG
                     VSEGISMSMQALLDNDDEVLVPMPDYPLWTACVSLAGGNAVHYICDEEANWYPDIDDI
                     KSKITSKTKAIVLINPNNPTGAVYPREILQEIVDIARQNDLIIFSDEVYDRLVMDGME
                     HIPIASIAEDIFTVTLSGLSKSHRICGFRVGWMVLSGPRQHVKGYIEGLNMLANMRLC
                     SNVLAQQVIQTSLGGQQSIDSMLLPGGRIYEQRNYIHKAINEIPGLSAVKPNAGLYLF
                     PKIDTDMYRIDNDEEFVLNFLKQEKVLLTHGRGFNMNTADHFRIVYLPRVDELTELQE
                     KMARFLSQYKR"
     gene            54266..54718
                     /locus_tag="VBC28_000056"
     CDS             54266..54718
                     /locus_tag="VBC28_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEO6814068.1"
                     /translation="MSQKYERILVAIDGSTESELAFEKAVNVALRNDSELILTHVIDT
                     RALQSFATFDTYIYEKLEKEAKDVLEEYEKQAREKGADKVRQVIEFGNPKTLLAHDIP
                     EKEKVDLIMVGATGLNTFERFLIGSSSEYILRHAKVDLLIVRDPNKTM"
     gene            complement(54774..56156)
                     /locus_tag="VBC28_000057"
     CDS             complement(54774..56156)
                     /locus_tag="VBC28_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HEO6814069.1"
                     /translation="MAIKAVFFDIDGTLLNDRKNVQKSTIKAIRNLKDQGILVGLATG
                     RGPSFVQPFLENLGLDFAVTYNGQYIYSRSEIIYTNQLSKTTVYRLIRYAGARRREIS
                     LGTASGLLGSGIIGLGTSRLGQIVSSLVPRKWAKAIERSFKHFIRRIKPQNIDSLMVI
                     LREPIYQVVLVATEGESERIQKQFPRVKLTRSSPYSMDVISEGQSKVKGIERVGQRYG
                     FDLSEVIAFGDSDNDIEMLSQVGIGVAMGNASQQVRENARYTTADNNDDGISKALAHY
                     GLIQFEIEKTFSSRDENFNKVKSFHLLMDGETIETPRLYDSKEAGFRSDFKVEEIVEF
                     LYAASQGNQKVFDQSIRNLHLAIDKARDKVISKDHPETPLVGEVDALTDLLYLTYGSF
                     VLMGVDPKPLFDTVHEANMGKIFPDGKAHFDPVTHKILKPDDWEEHFAPEPSIRRELD
                     SQIQKSLNRK"
     gene            56225..57187
                     /locus_tag="VBC28_000058"
     CDS             56225..57187
                     /locus_tag="VBC28_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEO6814070.1"
                     /translation="MKKILVLHTGGTISMNANEKGQVMSSADNPMKYVDLSLDDLDLT
                     VVDFLNLPSPQITPHHMLDIYHYLKQHASNFDGVVITHGTDTLEETAYFLDTMILPKI
                     PIIITGAMRSTNELGSDGVYNYLSALRVANSTKAADKGVLVVMNDEIHAAKYVTKTHT
                     TNVSTFQTPTHGPLGIIMKQDLLFFKATEERVRFDLDKITGTVPIVKAYAGMGDSGII
                     SFLNSQNISGLVIEALGAGNMPPKAAQEIEELIEQGVPVVLVSRCFNGIAEPVYGYEG
                     GGAKLQESGVMFVKELNAPKARLKLLIALNAGLTGQNLKDYIEG"
     gene            complement(57222..58100)
                     /gene="aroE"
                     /locus_tag="VBC28_000059"
     CDS             complement(57222..58100)
                     /gene="aroE"
                     /locus_tag="VBC28_000059"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEO6814071.1"
                     /translation="MNSLNGETLLTGLIANPARHSLSPLMWNTSFQEKNMNYAYLTFE
                     VEEGKLTEAVRGVRALGIRGVNVSMPFKQSVIPLLDDLSPQAKLVGAVNTIVNQGGTG
                     RLVGHMTDGIGCFKALAAQGFSAKNKIITIAGIGGSGKAVAVQAAMEGVAEIRLFNRN
                     SSNYDKVIDLSDKIKKQFQIKVVVDYLENKTAFKDAIRTSHFYIDATSLGMRPLDNYS
                     LINDPEILTPNLVVVDLVYKPKETALLRFVRQNGVKHAYNGLGMLIYQGAEAFQLITN
                     QEMPISSVERVLQTEN"
     gene            complement(58198..59112)
                     /locus_tag="VBC28_000060"
     CDS             complement(58198..59112)
                     /locus_tag="VBC28_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEO6814072.1"
                     /translation="MLQNIGQTGIQATRIALGCMRMSDLKGKQAEEVVGTALDLGINF
                     FDHADIYGGGLSELRFRDAIKHLNVNRDKMIIQSKCGIREGYFDFSKEYILSSVDGIL
                     ERLGTEYLDFLILHRPDVLVEPEEVAEAFTKLRAEGKVKHFGVSNQNRFQMELLQSYL
                     DEPLAVNQLQLSPAHTPMFDAGLNVNMLNKASIEHDDGIVDYCRLKRVTIQAWSPFQI
                     DLSRGLFVNHPDYKELNETIAKLAKNYNVSSEAIVIAWILRHPAKMQAIVGSMNPSRL
                     KAIDKANDIALTRKEWYDIYRSAGNILP"
     gene            complement(59403..61418)
                     /gene="recG"
                     /locus_tag="VBC28_000061"
     CDS             complement(59403..61418)
                     /gene="recG"
                     /locus_tag="VBC28_000061"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000926642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEO6814073.1"
                     /translation="MLLQSPISNLKGFGPKSAEKFQKLDIYTVEDLLLYYPFRYEDFK
                     SKSVFDLVDGEKAVITGLVVTPANVQYYGFKRNRLSFKLRQGEAVLNVSFFNQPYLAD
                     KIELGQEVAVFGKWDATKSAITGMKVLAQVEDDMQPVYRVAQGISQSTLIKAIKSAFE
                     ISAHLELKENLPATLLEKYRLMGRSQACLAMHFPKDITEYKQALRRIKFEELFYFQMN
                     LQVLKSENKSETNGLPILYSKHAMETKISSLPFILTNAQKRSLDEILSDMSSGAHMNR
                     LLQGDVGSGKTVIAGLSMYAAYTAGFQSALMVPTEILAEQHYISLQELFPDLSIAILT
                     SGMKAAVKRTVLAAIANGSVDMIVGTHALIQDSVQYHKLGLVITDEQHRFGVKQRRIF
                     REKGENPDVLMMTATPIPRTLAITAFGEMDVSIIDELPAGRKPIITRWVKHEQLGTVL
                     EWVKGELQKDAQVYVISPLIEESEALDLKNAVALHAELSTYFEGIAKVALVHGRMKND
                     EKDAIMQDFKDKKSHILVSTTVIEVGVNVPNATIMIIMDADRFGLSQLHQLRGRVGRG
                     YKQSYAVLVANPKTDSGKKRMTIMTETTDGFVLAESDLKMRGSGEIFGTRQSGIPEFQ
                     VADIVEDYPILEEARRVASDIVKDNNWKENTEWALILDNLRQHSDFD"
     gene            complement(61498..63036)
                     /locus_tag="VBC28_000062"
     CDS             complement(61498..63036)
                     /locus_tag="VBC28_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEO6814074.1"
                     /translation="MSKQKVMATLLLSTLVLSLSSPLVTLAETINPETSLTMATASTE
                     SSSEAEKQEKTQPTDSETASPSAEGSISTEKTEIGTTETSSSNESSSSSSHQSSSNED
                     AKTSDSASTASTPSTNTTNSSQADSKPGQSTKTELKPEPTLPLVEPKITPAPSQIESV
                     QTNQNASVPALSFDDNLLSTPISPVTATPFYVEHWSGQDAYSHYLLSHRYGIKAEQLD
                     GYLKSLGIQYDSNRINGAKLLQWEKDSGLDVRAIVAIAVLESSLGTQGVAKMPGANMF
                     GYGAFDHDSSHASAYNDEEAIMLLTKNTIIKNNNSSFEIQDLKAQKLSSGQLNTVTEG
                     GVYYTDNSGTGKRRAQIMEDLDRWIDQHGGTPEIPAALKALSTASLADLPSGFSLSTA
                     VNTASYIASTYPWGECTWYVFNRAKELGYTFDPFMGNGGDWQHKAGFETTHSPKVGYA
                     VSFSPGQAGADGTYGHVAIVEEVKKDGSVLISESNAMGRGIVSYRTFSSAQAAQLTYV
                     IGHK"
     gene            complement(63129..64229)
                     /locus_tag="VBC28_000063"
     CDS             complement(63129..64229)
                     /locus_tag="VBC28_000063"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEO6814075.1"
                     /translation="MISSYHRPTRALIDLEAIANNVKSVQEHIPSDKKTFAVVKANAY
                     GHGAVEVSKYIESIVDGFCVSNLDEAIELRQAGIVKMILVLGVVMPEQVILAKNENIT
                     LTVASLEWLRLCQTSAVDLSGLEVHIKVDSGMGRIGVRQLDEGNKLISELGESGASVK
                     GIFTHFATADEADNCKFNQQLTFFKDFISGLDNCPDLVHASNSATSLWHSETIFNAVR
                     LGVVMYGLNPSGTDLDLPYPINPALSLESELVHVKQLHDGSQVGYGATYQVTGDEFVG
                     TVPIGYADGWTRDMQGFSVIVNGELCEIIGRVSMDQMTIRLPQKYTIGTKVTLIGQQG
                     SCNITTTDVAQKRQTINYEVLCLLSDRIPRYY"
     gene            complement(64226..64585)
                     /locus_tag="VBC28_000064"
     CDS             complement(64226..64585)
                     /locus_tag="VBC28_000064"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000635015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEO6814076.1"
                     /translation="MIVGHGIDLQEIEAITKAYERNQRFAERVLTEQELLLFKGISNP
                     KRQMSFLTGRWAAKEAYSKALGTGIGKVNFHDIEILSDDKGAPLITKEPFNGKSFVSI
                     SHSGNYAQASVILEEEK"
     gene            complement(64610..65617)
                     /locus_tag="VBC28_000065"
     CDS             complement(64610..65617)
                     /locus_tag="VBC28_000065"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEO6814077.1"
                     /translation="MGFHQLSDKINIEILKQKTSLDLEVSQKKLAKEEELKNIIKGED
                     QRFLVIVGPCSADNPKAVLTYAKRLAKLEAAFKDKMFLVMRVYTAKPRTNGDGYKGLV
                     HHSDKLGVFFQARKMHYDIIRETGLLTADELLYPEMLSVMDDLVSYYAIGARSVEDQG
                     HRFISSGIDAPVGMKNPTSGNLRVMFNAVYAAQNQQELFYQNKQVRTDGNLLSHVILR
                     GYHNADYRSIPNYHYENLLETITHYEETDLQNPFIVVDTNHDNSGKQFLEQIRIVKSV
                     LADRQWHTKIRNYVRGFLIESYLEDGRQDKPDVFGKSITDPCLGWDKTEMLIRDIYNS
                     L"
     gene            complement(65743..68271)
                     /gene="secA"
                     /locus_tag="VBC28_000066"
     CDS             complement(65743..68271)
                     /gene="secA"
                     /locus_tag="VBC28_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEO6814078.1"
                     /translation="MANILRTVIENDKGELKKLDKIAKKVDSYADHMAALSDEALQAK
                     TPEFKERYQNGETLDQLLPEAFAVVREASKRVLGLYPYHVQIMGGIVLHHGDIPEMRT
                     GEGKTLTATMPVYLNAISGLGVHVITVNEYLSTRDATEMGEVYSWLGLSVGINLAAKS
                     PFEKREAYNCDITYSTNAEVGFDYLRDNMVVRQEDMVQRPLNYALVDEVDSVLIDEAR
                     TPLIVSGPVSSEMNQLYTRADMFVKTLNSDDYIIDVPTKTIGLSDTGIDKAENYFHLN
                     NLYDLENVALTHYIDNALRANYIMLLNIDYVVSEEQEILIVDQFTGRTMEGRRFSDGL
                     HQAIEAKESVPIQEESKTSASITYQNMFRMYHKLAGMTGTGKTEEEEFREIYNMRVIP
                     IPTNRPVQRIDHSDLLYPTLDSKFRAVVADVKERYEQGQPVLVGTVAVETSDLISRKL
                     VAAGVPHEVLNAKNHFKEAQIIMNAGQRGAVTIATNMAGRGTDIKLGEGVRELGGLCV
                     IGTERHESRRIDNQLRGRSGRQGDPGESQFYLSLEDDLMRRFGTDRIKVVLERMNLAE
                     DDTVIKSKMLTRQVESAQRRVEGNNYDTRKQVLQYDDVMREQREIIYANRREVITAER
                     DLGPELKGMIKRTIKRAVDAHSRSDKNTAAEAIVNFARSALLDEEAITVSELRGLKEA
                     EIKELLYERALAVYEQQIAKLKDPEAIIEFQKVLILMVVDNQWTEHIDALDQLRNSVG
                     LRGYAQNNPIVEYQSEGFRMFQDMIGSIEFDVTRTLMKAQIHEQERERASQHATTTAE
                     QNISAQHVPMNNESPEYQGIKRNDKCPCGSGMKFKNCHGLRCLQ"
     gene            complement(68380..69327)
                     /gene="manA"
                     /locus_tag="VBC28_000067"
     CDS             complement(68380..69327)
                     /gene="manA"
                     /locus_tag="VBC28_000067"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001294260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HEO6814079.1"
                     /translation="MSEPLFLEASMHDKIWGGTKLRDEFGYDIPSETTGEYWAISAHP
                     NGVSRVKNGRFKGCFLDKLYQGEKSLFGNPDDTVFPLLTKILDANDWLSVQVHPDDAY
                     ALKHEGELGKTECWYIISADEGSEIIYGHNAKTKEELRQMIESGDWEHLLTRIPVKSG
                     DFYYVPSGTMHAIGKGILILETQQSSDTTYRVYDFDRPDASGKLRDLHIEQSIDVLTI
                     GKPANTVPANMKLKHLSSTLLVSNDFFTVYKWEISGVTNFKQFAPYLLVSVLDGAGHI
                     TVDNKVYTLKKGDHFILPNDVVKWDIDGQLEIIASHVKK"
     gene            complement(69445..70326)
                     /locus_tag="VBC28_000068"
     CDS             complement(69445..70326)
                     /locus_tag="VBC28_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEO6814080.1"
                     /translation="MTKLYGSIEAGGTKFVCAVGDEELKVVEKMQFPTTTPQETIKKT
                     VDFFKRFEKKLEAVAIGSFGPIDIDKKSKTYGYITTTPKLHWANVDLLGLISKDFNVP
                     FYFTTDVNSSAYGEVIARNNIDSLVYYTIGTGIGAGAIQKGEFIGGTGHTEAGHTYMA
                     MHPQDQANDFKGICPFHNSCLEGLASGPTLEARTGIRGELIEENSMVWDVQAYYIAQA
                     AIQATVLYRPQVIVFGGGVMAQEHMLRRVRQTFATLLNGYLPVPDLSDYIVTPAIEEN
                     GSATLGNFALAKKISKG"
     gene            complement(70394..72313)
                     /locus_tag="VBC28_000069"
     CDS             complement(70394..72313)
                     /locus_tag="VBC28_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEO6814081.1"
                     /translation="MASNTEIAKQVINAIGGASNVRSVAHCATRLRVMVKDETVIDKN
                     TVENIEKVQGAFFNSGQYQIIFGTGTVNKIYDEVVAQGLPTSSTSDQKAEAAKQGNAF
                     QRAIRTFGDVFVPLLPAIVATGLFMGIRGAINNDTVLALFGTTSKAFSSSNFYTYTVV
                     LTDTAFAFFPALISWSAFRVFGGNPVIGLVLGLMMVNSALPNAWAVASGDAHPIKFFG
                     FIPVVGYQNSVLPAFFVGLLGAKLEKWLHKKIPDVLDLLLVPFLTFTVMSILALFVIG
                     PIFHSVENYVLAGTKFVLNLPLGLSGLILGGVHQIIVVTGVHHIFNLLEAQLIAADGK
                     DPFNAIITAAMTAQAGATLAVGVKTKNKKLKALAFPAALSAGLGITEPAIFGVNLRFG
                     KPFIMGLIAGAAGGWLASILKLAGTGFGITIIPGTLLYLNGQIVKYLIMVIGTTSLAF
                     VLTYMFGYEDKDEKAVAEVSPLVEETDDDPTITQTSQLRAETIVSPLDGQVIALDTVS
                     DPVFSSGIMGDGLAIKPRGNTIYSPVDGFVQIAFETGHAYGIKSDKGAEILIHIGIDT
                     VTMNGTGFTSKVKADQKVKKGDILGTFDSAKIAEAGLDNTAMIIVTNTADFADVVPLV
                     KEGVVTTGDMIIDVK"
     gene            72498..73937
                     /locus_tag="VBC28_000070"
     CDS             72498..73937
                     /locus_tag="VBC28_000070"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HEO6814082.1"
                     /translation="MNLPTEIRYRPYDEWTEEDKENIVKNVSKSPWRATYHLEAKTGL
                     LNDPNGFSYFNGKFHLFYQNWPFGAAHGLKQWVHTESDDLVHFKETGIKLKPDHVNDS
                     HGAYSGSALAIDDKLFLFYTGNVRDMKWNRDPRQIGAWMTNDGKITKFDKVLISQPND
                     VTEHFRDPQIFNYDNQFYAVIGAQNSKKCGFIKLYKALNNDIHHWEFVGDLDFGGTGS
                     EYMIECPNIIFVKGKPVLLYSPQGLDKNELDYQNIYPNTYKIGQYFDANSSKIVEPSP
                     IYNLDYGFEAYATQGFNTSDGRAFIVSWIGLPDIDYPSDQFDYQGAMSLVKELSIKNG
                     NLYQYPVPAMKNLRQHQAEFKTQLQTNNTYELELLVPRNDLSSFVLFANPKGQGLSIT
                     IDTVKGKVIIDRSQAGQQYATEFGTSRQCDIPKDATSINIFIDKSIFEIFINKGEKVF
                     TGRVFPDAEQSGIQLKEGHVHGKYFELKY"
     gene            73939..74901
                     /locus_tag="VBC28_000071"
     CDS             73939..74901
                     /locus_tag="VBC28_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEO6814083.1"
                     /translation="MVAKLTDVAALAGVSPTTVSRVINKKGYLSQKTVTKVNEAMRTL
                     GYKPNNLARSLQGKSAKLIGLIFPNIRNIFYAELIEHLEIELFKHGYKTILCNSEKDP
                     IKEKEYLEMLGANQVDGIISSSHNLGIDDYEKVEAPIVAFDRNLAPHIPIVSSDNFFG
                     GKMAAQTLKKHGCQKMIMITGNDNSDSPTGLRRLGFSYESKESKVITVTNGLSNMRRE
                     MELKSIISTHKPDGIFTSDDLTALLVIKLISQLGLSIPEDIKVIGYDGTSFIQDYVPH
                     LTTIKQPIREIAQLMVEILLAKIEGQKTNKDYILPVSLIPGSSV"
     gene            complement(74988..75422)
                     /gene="nusB"
                     /locus_tag="VBC28_000072"
     CDS             complement(74988..75422)
                     /gene="nusB"
                     /locus_tag="VBC28_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000204941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEO6814084.1"
                     /translation="MTSVFKDSRRDLRERAFQTLFSLETGGEFIDAAHFAYGYDKTVS
                     EDKVLEVPIFLLNLVNGVVDHKDELDTLISSHLKSGWSLERLTLVDKSLLRLGLYEIK
                     YFDETPDRVALNEIIEIAKKYSDETSAKFVNGLLSQFITNEN"
     gene            complement(75415..75804)
                     /locus_tag="VBC28_000073"
     CDS             complement(75415..75804)
                     /locus_tag="VBC28_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEO6814085.1"
                     /translation="MTTENLGEIVISPRVLEVITGIAATKVDGVHSLRNKAVTDSLSK
                     KSLGRGVYLKNEEDDTVAADIYVYLQYGVNVPAVSIAIQQAVKTAVYDMAEVKISSVN
                     IHVEGIVPEKTPKPDLKSLFDEDFLDD"
     gene            complement(75893..76453)
                     /gene="efp"
                     /locus_tag="VBC28_000074"
     CDS             complement(75893..76453)
                     /gene="efp"
                     /locus_tag="VBC28_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEO6814086.1"
                     /translation="MIEASKLKAGMTFETADGKLIRVLEASHHKPGKGNTIMRMKLRD
                     VRTGSTFDTSYRPEEKFEQAIIETVPAQYLYKMDDTAYFMNNETYDQYEIPTVNIENE
                     LLYILENSEVKIQFYGTEVIGVQIPTTVELTVAETQPSIKGATVTGSGKPATMETGLV
                     VNVPDFIEAGQKLVINTAEGTYVSRA"
     gene            complement(77381..77680)
                     /locus_tag="VBC28_000075"
     CDS             complement(77381..77680)
                     /locus_tag="VBC28_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000926392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4176 domain-containing protein"
                     /protein_id="HEO6814087.1"
                     /translation="MLLPVGSVVYLIDGNQKLVIVNRGAIVEQEGQEVYFDYLGGIFP
                     EGLNLEQVYYFNQEDIDEVVFEGYHDEEEERVSRLIEKWKNTEGKNLPKGKTSVE"
BASE COUNT        24609 a        15457 c        12214 g        25518 t
ORIGIN      
        1 aagcgagaga ccactttctt ttttatatta tagtcacatc atcatattta tttagaaact
       61 ccaagtatct ctcgaatatc atctacgctc atgctagcat gggtttcatt tccatccaga
      121 actgttgtaa ccaaatgttt tttagtttct tgcatttcta gaattttttc ttcgattgtg
      181 cctctagtaa tcaagcgata aacctcgaca ttctcttttt gccccaacct atgtgcgcga
      241 ctaatcgcct gcatttcaac agctggattc caccacaagt caatcagaac aacggtatca
      301 gctcctgtaa ggttgagacc aacgccacca gcttttaaag aaatcaagaa agcatctttt
      361 gatcctgcat taaatgctct ggtcatttca tgacgttcat ttgctggtgt agaaccagta
      421 attttataag ttgtaagccc catagctacc atctcacgtt ctgcgatatc taacataccc
      481 ctgaactgag aaaaaatcag agcacgatgt ccattttcct taatttgtgt taaaagttga
      541 cgtaggcttt ctaacttacc gctttcacca tcataatcca taaaaagacg tggagtgtca
      601 caaatttgac gtaatcgtgt gatgccagat aaaatttcaa ttttacgacg atttaagtca
      661 gcgtcactag aatgttggat actttcttga atttggcgta attgcgccaa atagataact
      721 ttttgactgt ctgtcatttc atttggatag ttcatttcaa tcaaatccgg taattctggg
      781 agaacttctt cttttcgacg tctcatcaca aatggtttga tataacgagc aacttgttta
      841 gggttcaact tcaaaaactc tttcttacca ggtaataaac caggtaaaat gatctggaaa
      901 atggaccaaa tttctaacag tttattctca ataggtgttc ccgataatgc aaaacagttt
      961 ttgacctcaa aagatctcaa gctatgggct attttagttt gagcattttt cataacctgt
     1021 gcttcatcca aaattaaata atcataattt ccttcagaat aagtctcaaa atcctgccta
     1081 aatgatgaat aacttgtaat gattacttga tgacgttcag caataatttc atctctacga
     1141 atcttagaac cataggcaac tgctacatct acgtctggcg caaatttgtg gaattcatct
     1201 tgccaattat aaattaaact tgatggtgat aaaataagca cacgagaatc tctagttagt
     1261 tttgtactta aaaatgaaat cgtttgaagt gtttttccaa gccccatatc atcagctaaa
     1321 atccctgcaa agccataatg atctaacata cttaaccaac ggacacctgt aagctgataa
     1381 tcgcgcaagg aagctgaaac agatagacca ggtattttaa acttttctgg gtgtctcaaa
     1441 tcttcaatca attggtaaaa atgttgtgaa aagctaacat tatcttgatt tgcaaacagt
     1501 tctgataatt gaaatgctgc aatattatct aattcaatat gcccattctt tgctcgtcta
     1561 gctctcaacc cttgcaaagt tgcacttact ttcttagttt cttcatcaaa aataactaat
     1621 tgccctgatt tattcacaaa ataaggatta ttgtcaaaca aagctaccag agcttgatca
     1681 acatcgtttt ccagtaaatc agagaaatca aaagaaatat ctagcaggct tcccttagct
     1741 tcaatatcaa tttgcggtcg ttctatgaga cgataacttt ccaattttgc ggataagctc
     1801 actgttccca tttttttaaa ttgtggaagc atataggtat aaaaatcgta taattcttgt
     1861 gcactgtatg ctggatggct ggttgaaaag tggggtgcaa agccatattt ttctaagagt
     1921 ttaaatactt tttcttcatg tttgaagtga ctagcaaatg ttaactgttc tagttcttga
     1981 cgattatgaa cagttaaatc attaccataa tcaaatacca tttgcaataa aatttcattt
     2041 tgactattaa tatcgaactc aaaatttacc ttaaaatcat gaatagagaa gctccgtgga
     2101 gcatccacta gtccaatctc cttaaaatcc agtaaatgag ctgctaactt atcttgatca
     2161 tctaaatcaa aatggatatg ttttgctaaa tctccctcaa tgggaagact acgaatagcc
     2221 gttaccatct tttcttgctt caaattaaga tggtagaaag tatcgcgata aaacaaataa
     2281 gaattcgcaa atagtggttt taagtctttc tctttgattt ctaattcaat tgattgccta
     2341 tggacaataa ccttaaattg ataaagatga tcctcacctt ctaattcctt aaagtagagg
     2401 tgattaaatt gatgaaaatc actttcaaat gaaaaatttt ctaaagcatt catcaaagta
     2461 acaccctctt caaaaaaacc acgagtgaga ctaaggtgac gaccttgatt tggtaaaata
     2521 aactctagat caattttgct tgaatgactt ggaatgagcc tccataaaaa ttctatcaat
     2581 tcttgactgg tctcatcaaa ctgtattaaa gacaaagttt caaagtattg ttttccaatc
     2641 tgataataag cttcttttcg aacagtattt aaaaaggctt tgatgtcacg aataacataa
     2701 cttctatcat caggcagtcg tctaattttt aaagtccacc agatatcatt agcataagga
     2761 ttttgttctc cttgagccga taattgatat tttatagtat catcttcatt aataattaga
     2821 ctatctaaaa aaacactacc aaaagaggtt ttcttcttag tttcttgttg actttcttgt
     2881 tgaacttgaa ctttagataa gattgcttta ccttctggat cgtttttcaa gaaatgctcc
     2941 agagcagcta aatgttgaca atagcctttt cttgcaaaaa aatcacaaga acattttgtt
     3001 tcttctgtta ctaatgaata ttcaatctga caatccccaa ctttagcttt taatagatta
     3061 ccttcttgag aaatcaagga caccaaacct tgttcgtaga gttctatacc ttgatttcga
     3121 atacgtcctg gaatcattct actcattatg ctaccacctt catcttatcc cttaattata
     3181 acaaaattca agtccaattt cttgtaaaaa tactaaattg attgtttttt gatacagtaa
     3241 caaataaaaa aggctaaaac aactgtccta accttctatt tcaatttcaa caataagatg
     3301 ttatttacgt ttacgtgcta ttagattaat tggtgttcct tcaaaaacaa atgcttcacg
     3361 gatttgattt tctaagaaac gaagatatga aaagtgcatc aattcttctt cgttaacaaa
     3421 aacaacgaaa gttggtggtt taacagcaac ttgagttgcg tagaaaatct taagacgttt
     3481 tcctttatca gttggtgttg gattaatagc aatagcatcc ataatcacat catttaatac
     3541 agcagacgga atacgtttgt tttgactttc gctaatacgt ttaatcatat ctggtaactt
     3601 gtgcaaacgt tgctttgtct ctgcagaaac aaagataatt ggcgcataag acaagaattg
     3661 gaagttatca cgtatatcag cttcccactg actgacagta tgattgtctt tttcgatagt
     3721 atcccattta ttaactacaa taataatacc tttaccagtt tcatgtgcaa accctgcaat
     3781 acgcttgtca tactcacgaa taccttcctc tgcattaatc accataagaa caacatctga
     3841 gcggtcgatg gcgcgcatag aacgcataac agaatatttc tctgtgtttt catatacctt
     3901 accagattta cgcataccag cagtatcaat catagtatac tcttgcccct ggctatcaac
     3961 gaaattagta tcaatagcat cgcgcgtagt accagcaact gggctagcaa taacacggtc
     4021 ttccccaagg atggcattaa ttaaactaga tttcccaaca tttggacgtc caatcaaact
     4081 aaaacgaata atatctggat tttcattttc ctcttctacc ggaagatttt ccacaatagc
     4141 atccaatata tcaccagtac caataccatg aactgatgac aagggataag gatcccctaa
     4201 accaagagag tagaaatcgt aaatatcatt gcgcatctca ggattatcta ccttattaac
     4261 agctaaaata accggtttat tggtcttata aagaatacgt gagacatact catctgcatc
     4321 agtcacacct tctttacccg acacaacaaa cacaataaca tcagcctctg tcatagcaat
     4381 gtctgcttgg tgcttaattt gctccataaa cggtgcatca acatcatcaa tcccaccagt
     4441 atcaattaat gaaaacttac gatttagcca ctcacccgtt gtatagatac gatcacgagt
     4501 gactccttca acgtcctcaa caatggaaat acgttctcct gcaattctgt taaataatgt
     4561 tgattttcca acattcggac gaccaacaat agcaactgta ggtaatacca taattctcct
     4621 ctcactattg ggaatactcc caattccatt ctattttatt aacggcgatt cggtccttct
     4681 aagtggaatt ctatcgctaa ataccgtacg cgctccataa ctcgttttgc ttgccaagtt
     4741 tcgtcgcttc cttttatatt agcccatttt ctttccaaat cgttaaaact ataatttgac
     4801 gtaaagaaag tgggcaactc ttctagcata cgatgttgta atataacttg taaaatctcg
     4861 tcacgaaccc aggacgttgc ctgttcagcc ccaatatcat ctaaaatcaa aatcggaaca
     4921 cttttcacag catcaatttc atcttttaca gttccgcttg aaatagcatt tttgacatca
     4981 atagcaaaac tagggaaatg aagaagagtc gttgacacac cctttctctc tgataattcc
     5041 ctcgccattg ctgccattaa ataggatttc ccaaccccca tatctccata taaataaagc
     5101 cccttgtgat tatatgaagg ataagtctct acaaaatcaa gcagttgtga catagctttc
     5161 attcgactct cgttattaat atcaaagtca gtcattctga tattccgata ggacttgggt
     5221 agattaacta aattaatacg atcactgata gcttgttttt tttgtgcttc aattaactct
     5281 cgtgtttcta aataagaaac atctgcataa ccttcattca taaccaaaat aggctcatag
     5341 cctttagcaa tatattgact atccttattt ttaaatttct gacgctcaat caaaaattga
     5401 ttaaatttag acatgctgat atttatttca cgctgagaaa gttggtgggt tttaatgaac
     5461 tccgctacct gagcatctgc taaaatcatt tgaataagct catcatttgt attacgaggc
     5521 actctccctt gattttctaa tgcttgtccc actgatttca tgattcacca tcctttccta
     5581 atcgttctaa acgcttgagg gcatctgttt taaactgttc taattcaatt tctttttctg
     5641 ggctaacctc atctttataa tcaggattac tccattcagg aacattcgat tgtttcttct
     5701 tcgtttctgt tttagtcctt actttttgat ctgaaaaatc acgaatttta agcacagcat
     5761 cttcggccgt cataacattc tgaaaagcaa aatcattagc aactttcatg atataggcct
     5821 tatttaagtt agcagatttt gtcttattca aggtatataa aaccataaca ttaatgactt
     5881 cgtctaggaa attcattttt gccaaatcct caagtaaagt cttctcacca cttgttgtca
     5941 ctgccttacg gcttctcttg attttttcaa ggaacactag agcactatca tttttagatt
     6001 cccttagtat gaccttttca ttatttgtaa aactctgatt atctttaata tgctgcccct
     6061 cattttgttg aactttcatt cgctgaggcg caatcattcc attgatagct gtttgttttg
     6121 ctaattgata ggtatcatac caatttaaat gataaagttc tgatacacta taaataccta
     6181 agacatcatc tttttcattg ttaaaacgta agccatctcg catcatcaaa cgtttgaaac
     6241 tttccaagtc aaaaacattt ttactccggt ttacctcttg ttttggttgt cctaaatccg
     6301 agaaaacatc tgtaaatttt ttagagatat ctctagcatt tttaggtatt ttcatatcta
     6361 attctgccac tgctacctct cctatacgtt tttctaacaa acgacgatag atgggattag
     6421 ataaaaataa ctcatttgac atagcttgat tcaacttaac aaggtaagta cctgtggctt
     6481 ggtaaacagt caacaaatcc aaagctgtta acattaccaa tgcatcttga aatcttggca
     6541 tcccgtattg cagatgattg agaacctctg aaaatttatg acgtcgtatt ccgtcatcaa
     6601 aaaaatgaac aaaataatca tagagtgcca tggcatcact tcccataatt gggaaataca
     6661 attgtattaa actattacta tcataaacaa tcttattttg cttaatatat gtaaactcat
     6721 ctattggact catcacacgc taccactctt tttactccgt acgcgctttg taatttgttg
     6781 aagtagttct tcaatttcat ctacatcttt aaaagattta taaacactag caaagcgcac
     6841 ataagtaatc tcatctaatt cggcgagttc atccatcact aaatttccga tagtgatact
     6901 agaaacctca tcttcatact cactcctaat tttacgttcg atacgtaaga tacaattttc
     6961 aatatcttcg ctcgaaacag ggcgtttttg agcactctga ataataccat tcaaaatttt
     7021 atctcgagaa aattgctcgc gagtaccatc tttctttatg actaaaagag gcaattcttc
     7081 caaacgttca aatgttgtaa agcggttgcc acatttttcg cactctctcc ggcgtctgat
     7141 agtcgtacct tcttcagctt gtcggctatc aaccacacta gatttattat agccacattt
     7201 tggacaacgc atgtcaacac ccctattcta tcatatcatt taatcatctt attttaacac
     7261 aaattcagta ataacaaaag aagcgcctct cttatcacct catcatatca aaacacaaaa
     7321 taatccttat gccttttatc attaagcata aggatttttc ttatatttct ttagtttctt
     7381 caaatttttt tataggaatg cgaacaatga attcagtccc ttgatttagt tcacttttaa
     7441 tatccacctt taaatggtaa gcatccataa tttgtttaaa cactgaaagc ccgataccta
     7501 aaccagcttg tgtactttct ctgttacgtg atttatcagt tcgataaaaa cgatcaaaga
     7561 taaactcaat atcttcatct gagatacctt ctcctttatc ttttacaact actgtcgcaa
     7621 aatcattgtc tactgataaa acaacactga cttctttata actaggagag tacttgatag
     7681 cgttatcaat taagatcatg agagcttgct caaaatgtat cttatatatc tttccccaaa
     7741 cgatatctga aatattattt tcaaaaataa attgaaaatc ttctctcaaa atacggaaat
     7801 tttcaataac aactgatata gagcttgata aatctgtcac ttcatcttga tgaagttcca
     7861 agcttccttg gacgcgaatc atatttaaca tgtcattaat cattaaggac atacgatctg
     7921 cctcatggta tgccgctgct aaactttctt caagaatttc tggatcatct ttcccccaac
     7981 gttgtaataa accaatatga ccctttacaa cagcgacagg cgttcttaat tcatgactaa
     8041 catcactaat gaattgtgat tgtcgacgag tatagtcttc caactgatcc aacatcccat
     8101 caaaaatcct agacaattct ccaatttcgt cttctgaacg gaccttactt ctaatttcta
     8161 aattactagg attttcagat atctgatgca ttaagtcgtg taaattttta accggacgaa
     8221 caatacgctt agtagcacta ttaataacga ccaaggctaa aacagtccct aaaacttcca
     8281 tcataatcaa gaaaacaatg atgttttgcc tcatactata ataacgacct aaattataga
     8341 aaatttgaac gtatccaagc aactgtcctg ttttttgaga gtagattgct tttgtaccaa
     8401 caaaaccttc catatctgca actttagaga tgataacttt tcccatggat ttaccaatag
     8461 ttggcatacc cgttctcagt gtagaaaaaa ctggtttcct gttgacatcg tatagggtca
     8521 tttcttgatt aacataaaga atactatcga ttgactcgcc cgttctaaca attccatttt
     8581 gacgcttatg aggatagatg tcatccgcaa aaacctggtt gtacaggatc tctaaaatat
     8641 catcggatgt caaactagag ttcgcttgcg acaaacgaac tctagttttt tctaatgacc
     8701 taccgacatt aattttttcc ctgtgaagga cgtatcttgt tgccgaagta taaactaaaa
     8761 cagaaaatac tgtaaatagg ctaaaaaata agataaaaac taattgcgat aatttttttg
     8821 acagaggttg ttttacccct ataaactgat cttttttatt tttcacgaat cacatagccc
     8881 attccgcgaa cagtttgaat atatgattca cgacctggga tatcaatttt acctcttagg
     8941 taacggatat aaacatcaac aacgtttgtc tctgctgcca catcgtattt ccaaacatgt
     9001 tctagcaact cttctcgtgt cataacacga ttcatatttg tcatcaagac attcaacaaa
     9061 tcaaattcac gttttgttag tgaaatttcc tcatcaccac gcatcgctga acgattatga
     9121 gtatttaatg acaaatcacg gaaactacca ctatcacctt tttcttttgt cttcgtttca
     9181 atttcttggc gtcggaaaat cgctctaaca cgtgctaata attcttcgat tgcaaacggc
     9241 ttaacaatat aatcatctgc tccacgatca agaccagcta caatatccat aacagaatca
     9301 cgtgctgtca tcatcatgat ataggttgtt ttttcagcct gcaggcgacg tgtgatttcg
     9361 aaaccatcca tctctggaag cattaaatcc agtagaatca aatcaaaatc tttttctaat
     9421 gctgtgtcca atccttcacg accgtttgtt tcaacaacaa catcatatcc ttcatgtagt
     9481 agttctaacg agacgaagcg agctaaattt ttctcatctt cgattattaa gatcttttta
     9541 cccatctaca ccaaccttct tattctttac ttaacactat caattatttt ttagaatatc
     9601 tagataaaaa acaagatagc ccacatttta ctaacgtttc tttaaataaa taaatcgcca
     9661 tcccaaacaa ttgttcgata gcgagactta atttttatta ttgagtagtg tcctctgtca
     9721 cttttcaatt ctactaaaaa aaaattaaaa tatcaactga tgttattaat aatttttatc
     9781 taatcactat caaacatacc ttctaaggct gcgaaaggat tagatttttc tttttcttta
     9841 gcttgttttt cttcatactg cttttcagtc attaacgacc aatttttacc tgataaatcc
     9901 gcttctaccc caacctcatc agctgccaga acacgaagag gtatatttaa caagatatta
     9961 tcgataacgc tttcttctaa attaatatca tcttcctcta atattaagac caactcctga
    10021 tcaacgagct cttttttagt gctaacattt tcagcttcaa taaaaacttc attaatagtt
    10081 tgcttttcag ataaaacaac tggtttcata gaacgactag acggtaatgt aatatcgtaa
    10141 gccatattat agtcaagtag atagagacca tcttcataca ccacatgacc tgaaacttga
    10201 atatcagaaa tatccattat ctctgaatga cgctccataa gcgactcttt gatatctatt
    10261 tttttatcaa aataaagccc ctctggtgat tttttgattt ctgtaagtaa cacgtgccat
    10321 ctccctttat agtatatgtc atattatatc aagttttaca ggctttgtct taaaaaaaca
    10381 aaacgcaagg catagctacc ctacgttttg ttaacgacta catatgacgc aaacgctcaa
    10441 tacgatctgc aattggtgga tgcgtataaa ataaagactt taacccttct tttttcgttg
    10501 ggtcattgat atagagagcc gcactagcat catctactgg atgccccata ggttcagaac
    10561 gatcaagttt ttctaaagca gagatcattc cttgtggatt tcttgttaac tcaacagaac
    10621 tagcgtctgc taagtattcg cgctgtcttg aaatagctaa ttgaactaaa ctagcggcta
    10681 atggtgcaag gattagtgat aaaatagaga aaatcaaaac caagatgttt ccaccatctt
    10741 ctctgtcatc atcacgccta cgacctccgc cgtaaaataa catacggcta ccaatgcttg
    10801 atattagagt tacagcactc gctaaagcta ctgcaatggt tgaaatacgg atatcataat
    10861 tacgaatatg actaacctca tgtccaataa caccctctag ctcttcgcgg ttcataacag
    10921 ctaataggcc tgtcgttgct gcaacagcag cattttctgg cttagagcct gtggcaaagg
    10981 catttaggga gtcatcttca acaatgaaaa cgcgtggcat aggtatctga gctatcattg
    11041 ccatatcttc aacaatatga aaataatttg gcgcctcatc ttctgtaact tcacgcgcat
    11101 tgttcattga cataacaaca ttagttgatt gaaaaatcat acttactgca tagataaccc
    11161 caataattag agctagaaca agcccaaatt ggtagctgcc taataccaaa taacctacag
    11221 ctgctccaat agctgctaaa agacagaaga agacaattaa caagacaact gtttttctct
    11281 tattacttgc aatttgttga tacagcaaaa gcctctctcc cttctcaatt aaaagtcaaa
    11341 ggaaactttt ggcacttctt tttcttcttc aggagtttct aagaattggc ttggtttgaa
    11401 cccaaatagt tttccaacaa tattacttgg gaaagtttct aatttcacat tataattgga
    11461 tgttgttgtg ttatataact gacgtgaata agaaatttta ttttccgtat tagttagttc
    11521 ttcttgaagt ttaacaaagc tattgttggc ttttaaatct gggtaatttt cagcaaccgc
    11581 taaaatactt gaaagctgtc ttgttagctc attacttgct gtcattgctt cagcaggtgt
    11641 gttagcttta gcaacttgag cacgcagttc tgcaatcttc tcaagtgttt tcccttcgta
    11701 cgcagcatag cctttaacag tttcgattag gtttggaatt aaatcgttac gacgtttcaa
    11761 ttgaacgtct atttgactcc atgattcttt agtatgcatg cgacttctaa ctaaactgtt
    11821 ataagcaaca atcaaccaaa taacaaacaa agcaataatt gctattaaaa tcatagttcc
    11881 catagtagta gctcctttac attttttcca ttatagcaat tttctttaga ttaggaaagt
    11941 cctgttattc ccattattag tatttgtgat aaaatagagt cattatacga tatagaaatt
    12001 gaggtttact atgacacccc aagcatttta tcaagttctg atagagcatg gtattacact
    12061 tactgacaaa cagaaaaaac aatttgaaac ttattttaga cttcttgttg aatggaatga
    12121 gaaaattaac ctaacagcta ttacagataa ggaagaggtt tacttaaagc acttctatga
    12181 ttctattgcg ccgattttac aaggttatat tgacaatagt cctcttagca ttttagatat
    12241 tggtgctggt gcaggttttc ctagtattcc tatgaaaatc ctttatcctg aaattgatat
    12301 tactattatt gattcactga ataaacgcat taattttcta aacatattag ctaatgaact
    12361 tgaactttca ggagttcact tcttccatgg gcgcgctgag gactttggtc aagatagagt
    12421 atttcgagct aaatttgata tcgtaacagc tcgtgcagta gctaaaatgc aggttctagc
    12481 agaattgaca ataccttttc ttaaagttaa tggtcgcttg attgcactga aagctgcagc
    12541 tgctgaagag gaattaattt ctgcagaaaa agctttgaaa acactatttt cacaagtaac
    12601 tgtaaataaa aattataagt tacctaatgg tgatgaccga aatatcacaa tcgttagcaa
    12661 gaaaaaagaa acgcctaata aataccctcg taaagctggt actccaaaca agaagccttt
    12721 gtaaagttgg gagctagtat gaaacatttc tttgattaca agacaatgtc tgtcgcacgt
    12781 aaactcagca ttagttttat cgctgttatc ctattgggaa gtattttact ttcacttcca
    12841 atttttcaat atgctaacgc ccctaaaaca cattacattg atcacctttt cacgactgtt
    12901 tcaatggtat gtgtaactgg tttatctgtt tttcctatca gtaaggttta taacggttgg
    12961 gggcagattg tagctatttt actcatgcaa acagggggac ttggtttagt aacactaatg
    13021 tcgttgtctt actataccct aagacgtaaa atgtcgttaa atgatcagac ccttttgcag
    13081 tcagcgataa cttataacag ttcgacagat ttaaaaaaat atctctatat gatttttaaa
    13141 gtaactttaa ctttagaagt tttagcagcc agcattttag ccatagattt tattcctcga
    13201 tttggtttgg gtcatggtat ctttaatagt atattcttag ctgtctcagc cttttgtaat
    13261 gccggttttg ataatctagg ggcaacgagc ttagctcaat ttaaactaaa tcctttggtt
    13321 aacattatcg tttgtttctt gattatttca ggaggacttg gatttgccgt atggaaagat
    13381 ttaattgaag ctactataca aacatctcac aagggaccaa aattaatcaa gacatttcca
    13441 aaacgcctca gcaaccattc taaactggtt ttaaaaacaa caaccattat tttactaaca
    13501 ggaactttgc tttcttggtt gttagagttt ggaaatttta gaacaattgc taatttatct
    13561 ctacctaaac aactaatggt cagctttttc caaacagtta cgatgcgtac cgctggattt
    13621 tcaactattg attataccca aactgatttc gcaaccaacc ttgtttatat tatccaaatg
    13681 ttaattggag gagctcctgg tggaacggca ggaggattta aagtcactgt tatcgccatt
    13741 ttactcttac tatttaaagc cgaactgagt ggccaaagcc aagtgacttt tcattatcga
    13801 acaattcctt ccagtattat taaacaaaca ctatcgattt taactttctt ttttatcata
    13861 cttattagcg gctatctcct tcttctggaa ctcaatcccc atatagatcc tttttctctt
    13921 ttctttgagg catctagtgc cttagcaacc gtgggtgtaa caatgaacac taccaatcaa
    13981 ttaactctgg gcggtcgaat tgtaattatg ttcctcatgt ttattggtcg agtcggtcca
    14041 attaccgtac tgctatcaat tcttcagaaa aaagagaagg aaattcacta tgctgaaaca
    14101 gaaattattc taggttagaa aaggtctttt atgaaaacaa aaattattgg tgtacttggt
    14161 ctaggaatat ttggacaaac actcgcacaa gaactaagta actttgaaca agatgttatt
    14221 gctattgaca gcaatcctga aaatgtacaa gctgtcgccg aagttgttac aaaagcagct
    14281 atcggagaca ttactgattt agctttccta aaacacatcg ggatcagtga ctgtgatact
    14341 gttattattg ctacaggaaa cagtttagag agctcagtat tggccgtaat gcactgtaaa
    14401 aagttaggcg tcccacaagt tattgctaaa gctcgaaacc ttgtatacga agaagtactt
    14461 tatgaaattg gtgctgattt ggttatctct ccggagcgag aatctgggca aaatgttgct
    14521 gcaaacctca tgagaaataa aattacagat gtcttccaga ttgaatctga tatttctgtc
    14581 attgaattta aaattcccaa atcatgggta gggaaaactg tcgaacaatt aaatatccgt
    14641 cacaagtttg atcttaacct cataggtatc agaaaagcaa aaaacaaacc tgtcgatacc
    14701 gaagttccaa tcaatagtcc acttgaagaa ggtattattc tagtggctat cgctaactca
    14761 gatgccttcc aacgctatga ctatttagga tactttaact aaagaaaata aaaaactgac
    14821 tattaaggtc agttttttat tgccatgggt tatagaaacg tccatgatta aggtgaatta
    14881 aataaaggct tacaaacaga ctagctagag caagtatgat taagacaatg tcactttttt
    14941 ccaaagattg cttagagtac caagtacgtc ttttattttt accaaatcgt cgaagttcca
    15001 tagcagttgc tactgtatca atacgttcta gagagctaaa aattaatggt gtgacaatct
    15061 gcaaattccc cttaatgcgt gctactaagt tcgatttttt tgataattct attcctcttg
    15121 cctcttgtgc tcgacgtatt gtataaaatt cttcttgaac atcaggtata tatctaaggg
    15181 tcaaactaac agcataagct attttatatg ataaacctaa ttgatttaaa cttgatgcga
    15241 actgactagg attagttgtc atcaaaaaca aaagagctaa aggcactgta caaaagtatt
    15301 ttaaaatcag attaaagagg taaaacaact cttggctagt cacatcatat gggccaattc
    15361 cttttaacaa taaagaagaa ctaccataaa tcttatcacc ataatgggga gcaaaaagat
    15421 agaccattat aatatttaaa acagcgaata caacaactaa agtcataaca aaggacacct
    15481 gttgccattt gatttttgct aatctaaata agactagcga tcccaatgaa acaacaatta
    15541 aaaatcttgt atcataagtg gtcatgcaag caacagaaac taaaataaaa aatagtaact
    15601 tactggcacc tgatagcctg tatagaaagc cataaccctc ttgataacca attaattgtt
    15661 tagccattct tacctccttg attatcaatg taccattgtg ttagtttctg tggagatatc
    15721 cctaatctgt ctgctaaagc aaataaactc gttttcttta aatgagtctt tttcaaaagc
    15781 tctgtatttg aaaggatgtt aataggatga tcatctgcaa caatcagacc attgtcgatt
    15841 acaattgcac gatctgtata ctctaacata agctgcatat catgtgtaat cataacaatg
    15901 gtatgtccat cacaacttag tttatcaagg aaagacatca tttcagtata atgcttcata
    15961 tcttgaccag ctgttggttc atccaaaata ataacttctg gatttaaaat cagtatcgaa
    16021 gcaatcgtaa cacgtttttt ctgaccgaat gatagagccg atataggcca attcctaaat
    16081 tggtagagcc cacaaacctt taatatatcg tagacacgac tttctatatc attatcacta
    16141 aaacctctta aacggagacc cagagcaact tcatcaaaaa ccatcgcttg acttatcatc
    16201 tgattgggat tttgcaaaac atagccaatt cgttctgctc tttcttttat agagtcttga
    16261 ctgacatctt cattattata aattagagta gcattattag ggataaattg acataacgct
    16321 ttagctaaag tagatttacc tgcaccattt ttcccaacga tagctaaccg ctgaccttta
    16381 tataaatcaa acgaaacatc atctaaagta ttattctcta aatcatatga caccgacaaa
    16441 tgctgtactg ataataattt atctgtatgt ttatctaaat ctaatgctcg cattttaggt
    16501 atcaatagtt cactgaaatc aaagtcatca atagacgtca ttgtattttg tttttcaaag
    16561 tcatatccta aatttttgag agcagaaata tacagtggtt ctcgaatacc atattcagat
    16621 aaaaaattag tttttaaaag ttgatccggc tccccattat aaataatttg tccatcacta
    16681 aaaagaacta cacgatctac tcgctcataa aaaacatctt ccaatcgatg ttcaataata
    16741 atagtggtcg catcttgctc tcgatgaata cggtcaacca atgccatgat atcttgtccc
    16801 gattgtggat ctagattagc caaaggctca tcaaataata gaattgggct atcgtctact
    16861 aaaactccag ctaaactaac ccgttgtttt tgtcctcccg acaagtcttg tggacgttta
    16921 gacaaaagag gagctatttc taaacgtttt gcccacattt caacgataga ggccatttct
    16981 tcctgtgcta caacatcatt ttctaatgca aaagcaatat cttctgctac tgtcaatcct
    17041 ataaattggc catctggatc ttgtaaaacc gtagaaacta aatgagactt gtcatatatt
    17101 gacaagtcaa aagcattttt atggttaatc gtaaaaatac cactatattg gcctttatga
    17161 gtatttggga taataccatt taaacaatga ccaagcgttg acttccccga ccctgaaggt
    17221 cctaatatca agaccttttc acctttagga atgcttaagt taatcccttt taaagttggt
    17281 tcagattgaa cgtcatattg aaaggtaaaa tccttccatt caataaaatc tttcatagat
    17341 tactctctta tcctaatctt tggataaact acctttttgt ggtcgtgatt tagcgtatgc
    17401 tagcaacaaa agcgtaccac ctactccaat agtaatggaa ttcacaaggc ttgataagaa
    17461 accttgtgca aaaactttgc ttgcaggttc gctataaaag aaaatatcac catacggtgc
    17521 aacgacagac caacctataa ggttagcaat aacttgaact acattaaata gaagcaaatc
    17581 cttcttagtc atgccagata attgaatagc taatttttta gcaaaaaagc ctatcatcaa
    17641 acctaaaaga ccactaacta aaacccaagt ccaccacgga gaaccatatt gaatcgaatc
    17701 tttcaatgcg tgtccgataa aaccagtaaa aaaaccaact cctggtccat aaataactgc
    17761 aaataatgct aaaactgcat actgcaattg aatattagta tttggaattg gtgttggtat
    17821 attaactagc atacctataa tgataaaaag tgcagctcca attccagtcg ctacaacctt
    17881 ttttattgtg tttgtattca taaaaacctc tctgaaaaac aagcttaaac agtcacttac
    17941 aatctgttct attttatcat atttcaagct gatttgaaac cttattgaca agaagaactg
    18001 aaaaacgatt gaaaataata ttaaatacca aaaaagatct cagatagaat acctaagacc
    18061 ttcattagtt tatttttttc caaaaactga tttttcagct acagccgtga acagaacgtc
    18121 tgtcgatgaa tttaaggctg tctcacacga atcctgtatc acccctacaa tgaaaccaac
    18181 tcctacgact tgcattgcaa cgtcatttga aataccaaat aagctacatg ccaccggaat
    18241 caataaaagt gaaccaccag ttacaccaga agctccacat gctgatacag cagcaaccac
    18301 acttaataag aatgctgttg gaaaatcaac agtaattcct aaagtattta ctgctgctaa
    18361 agtcaggata ttaattgtaa tagctgcacc agccatattg atagctgcac caagcgggat
    18421 agatactgag taagtgtctt tatctaatcc taaatcctca catagtctca tattaacagg
    18481 aatattcgcc gcagaactac gtgtaaagaa agctgtaata cctgaatcct ttaagcatcg
    18541 taataccaaa gggtaaggat ttttacgcat cataacaaaa gcaatgattg ggttaacgac
    18601 aagagctaca aataacatcg tacccaccaa aactaatatt aaaagaccat aatcacctag
    18661 tataccaata ccgttttctg aaatgctagt gaaaaccaac cccataatac caattggggc
    18721 caaattgata atccatttga caacttgtga agtagcatca gcaaaagttt gcattaaatc
    18781 ttttgtacgc tgattactgc ttcgcatcgc caaaccaaaa ataaccgccc aaaatagaat
    18841 actcatataa ttggcctcgg taatggcatg aatgggatta tcaacaattt tcaacaaaag
    18901 agattgaaag acttctgaaa tccccttggg aggtaataag tctgtctttg ctggcgtttt
    18961 caatatcagt gtcaaaggga aaatatagtt cacaacaaca gcgattaggg ccgctgcaaa
    19021 agttgcagtg atatacaaac caattatcgt tgacatattg gtcttaccgc cctcacggtg
    19081 ttgcgacaag gcactaataa ctaaagtgaa aactaaaaga ggggcaatag ctttcaaccc
    19141 tcctacaaaa agttgaccaa gtaaaccaat cgcagaggct ttagggacta aaatacccaa
    19201 gaataagcca ataacgactc caattgttat tttttttatc aagcttgttt ttctccaagc
    19261 ttttaaaaat cgtttcatac taaactccta aaaaattctt aaactctatg atatgataaa
    19321 aaaaaagatt cgtcaaatac cttttttatt ttttagaggt tgacgaattc taaatttttc
    19381 tgatataatt actttttgaa atattcaatc aatttcgaaa ataaaatatt tttttgtaca
    19441 gaaaggtaca ttgatgaaac taaaaaacac ttatatcatt atcggcttga tgctttttgc
    19501 gctgttcttt ggagccggta atcttattta tccagcctac ttaggtatct actctggttc
    19561 taattgggcg tgggctatca ttgggttctg cttaacaggc gtaactttac ccctacttgg
    19621 tgttgcagct gtggcgtact caggagcaac cgatgttgaa agcttcacca aacctgtctc
    19681 taaaacatat gctattttct ttgcagtagc actataccta tctattggac ctttctttgc
    19741 cattcctcga acaggggcaa cctcattctc agttggtatt caacctatct taggcgacag
    19801 ctccttagtc aaaattatct atggtttaat tttctttggt atttcttatt tcttagctat
    19861 tagacctagt cagattgtag atcgtattgg taaatatcta actccctttt tactaactgt
    19921 tattagtatt ttaattattg cctcactcat ccatccagct gctatcgttg gaacaccaca
    19981 taacgcttca acgaatgtca gcgatgcttt caaggattta ccattcctag caggactgat
    20041 tcaggggtat ggtacaatgg atgcccttgc atcaattgct tttgctatta tcgttattca
    20101 agcaagtaaa cagtacggag caattaccaa aaaagaaatt acttctatgg cacttaaatc
    20161 tggtgctatc gctacatttc ttttagcttt tatttatatt tttgtaggac gtattggtgc
    20221 aacttcacaa agcttgttta aatttgcaaa tggttccttc ttacttcaca atactccaat
    20281 agacggtggg catgttctta gtcaatcagc taacttctat ttaggaattg tgggacaagc
    20341 tattttaggt acggctattt ttcttgcttg cttaacaaca gctactggat taatcactgc
    20401 ttgtgcggaa tacttccaca aattacttcc aaagattagt cacattactt gggctacaat
    20461 ctttacttta attgctatta cgttctattt tggtggttta tcagagatta tccgttggtc
    20521 actaccagtt ctctaccttc tctatccatt aactatagtc ttaattttct tagtcttttt
    20581 tgatcaaaaa tttgaaagta gccgcattgt ctaccaaact tctattgccg ccactgctgt
    20641 cgctgcttta tacgatgcct tatcaaaact cggcgaaatg actggtttat ttaccattcc
    20701 atccgctttg accacattct ttactaaagt tgttccttta ggtgaatatt ctatgggatg
    20761 gattagtttt gcaatttgtg gtgtcttagt tggtcttatt ctaaaaaaag taaaagcata
    20821 ataatttaaa gcagtacgaa aattactgct tttattttaa aataattgtt gtaaccgctt
    20881 tcttttggtg gtataatagc cttgaaaata aataattttt ggaggaactc taatatgaga
    20941 gctgcaactt atctttcagc taatgaatta tcacttactg ataaggctaa acctcaagta
    21001 ataaaaccca cagatgcggt tgtaagactt gtcaaaacaa ccatttgtgg tactgatcta
    21061 catatccttg gtggcgatgt tcctgcttgt aaaccaggaa caactttagg tcatgaaggc
    21121 attggtatcg tcgaagaagt tggttctgct gtcaatcatt ttaaagtcgg agataaagta
    21181 attatttctt gtgtcactgc ctgtaatact tgctattact gtaagcacca gttgccttca
    21241 cattgtgaag atggcgggtg gattttagga catcttattg aaggtactca agcagaatat
    21301 gttcatattc cacatgcaga tggtagcctt tatcatgcac ctgaaggtgt ttgtgacgat
    21361 gcattggtta tgttatctga tattttacca acttcatacg aaatcggcgt cttaccttct
    21421 cacattaaac caggtgatac tgtttgtatt gtaggcgctg gaccaattgg actatctgcc
    21481 ttactaactg cacaattcta ttctcctgcc aaaattatta tggttgattt atctcaaaaa
    21541 cgacttgaag cctctaaaaa atttggtgca acacacacca ttctatcaac gtctactcaa
    21601 gaagttaaag aagaaattga taaaattacc aaaggccgtg gcgttgatgt cgtattagaa
    21661 tgtgttggtt atcctgcaac atttgatatt tgtcaaaatg tagtttctat tggaggacat
    21721 atcgcaaatg ttggtgttca tggtaaacct gttgaattta acctccaaga tttatggata
    21781 aaaaatataa cccttaatac tggattagtt aatgctaata caactgaaat gctccttgaa
    21841 gttcttgaga caggcaaaat cgatgcaaca caactagtaa cacatcactt caagctatct
    21901 gaaatcgaag aagcttataa ggtatttaaa gctgctgaag aaaacaatac tctcaaagtt
    21961 atcatcgaaa acgatattac tccagcctga taatttttaa acaaataaaa atgtgagaag
    22021 aatagccttc tcacattttt atttatgact caagcgacgt gttaaaaaat cacctaaaaa
    22081 ttggataaag aaaattaata ataaaatcag aatagtcgct actaaagtaa tatcatcacg
    22141 agaatagtta tatcctttag taatagcaac agaacccaat cctcctgcac caatagcgcc
    22201 agccatagct gtttcaccta ctaaagaaat caaagtaacc gttgatactc gaattaaatc
    22261 tggtagacct tcacgaagat aaactacaat aatatcccaa agtgttccac ctgaggcttg
    22321 tgcagcctca ataactccac catcaagttc agctaaaaca acttgaactt gacgagcaaa
    22381 aaatgggaaa actgccaaag aaagaggtac caaagctgct ggtgaaccaa gtgttgttcc
    22441 tacaattacg cgagttactg gcgcaatcaa agcaagaaga ataatgaagg gcagagctct
    22501 aaaaacagaa acaactttat ctaaaactcc aaatactaat ttattagcaa taactcctct
    22561 aggactagtt aaaacaagga ataaacctcc taacaatccc attaaacctc caataaggaa
    22621 cgaccaaaaa gtcatataaa gggtttgtac aatagctgtc tgccagccgt aagcaccttc
    22681 ccaacccatt tgatatacat ttggtaaatg tgtttgaatc cactctatca tgctttacca
    22741 ccttctttca ataacgttaa ttgaacttgc gaatctgtaa ttgcttggca ggcttgtctt
    22801 aatttttctt cgtcacctga taagataact accatttctc caactggaat accatctaaa
    22861 atttcaatat tcccatagag gatgttagca gttacttcaa attctttata aatttgattt
    22921 aaaagcggct catccgtgct atgcccagcg tatttcagtt gaactaattt gctacctact
    22981 ggtagatttt tgacaacttc ttgttgctca atcttaagca ttgcctcatc aataccagta
    23041 gctattttta taaaatcttg tgtaagactc tctctaggat gtgaaaaaat gtctaaaact
    23101 gatccttctt caatcaattt accattttgc ataacagcaa cgcgattagc aatgtctttg
    23161 acaatttgca tctcatgtgt aatcaaaaca atcgtaagtc ccaatttttt attcagatct
    23221 tggagaaggg caagtatttg ttttgttgtt ttagggtcaa gtgcagaggt agactcatct
    23281 gaaattaaga ttttaggatc gttagccaat gcacgcgcaa tggcaacacg ctgtttttgc
    23341 cctcctgata attgtgaagg atagttttga gcacgatcgg ataatccaac aagttctaat
    23401 aatttagcaa ccttagctgc tttcgcctct tttgataaac ctgaatgttt aagagcaaaa
    23461 gctacattct gctctgctgt caactgtgac attaggttaa aatgttgaaa aatcattcca
    23521 atttcacggc gctgttcgcg taattgtgtg ctagtcagtg tcaccttgtt gtcgtaaata
    23581 acttggtcat caatagtaat tttacctgct gaaggttctt gtagcaaatt aataacacga
    23641 acaagggtag attttccggc tcctgaataa ccgacaattc catatatatc accttgatta
    23701 atatggatag taacatcttt aacagcatta atctcacgtt tcttttggtg gaaagtaaca
    23761 tcaatgttgt ctaatttaat aattggttct ttactcatat gattctatta aagcctccac
    23821 taattcaata tgggtgtaat aatcagcaat actaacattt tcatcaacgc catggtctcg
    23881 actattggca tgtcctatgc caatagctgc aattggcgct cttagagctt gatgaactaa
    23941 atacattggt cctgtacctg gtgaggtagg taacagagat atcccttctg gataaaattg
    24001 ctcagctaaa tcaacaacct ttaggataga tggcgctgac atatcactac gataagacat
    24061 ttcaccaagg gtataggtta gctcaatgtc tttaaagcca ttctctttca aatgattttg
    24121 tattgaatct agtacacctt tcggggttaa ccctggtact aacctaacct ctgctttaca
    24181 tttagcataa gcaggtaaaa ttgttttgac gccttcacct tgataacctg aggttatacc
    24241 ttcaatggct atagaaggtt caaaataaag ctttctcaaa aaagttttat gactatctgc
    24301 taaactgggc aaaactaatt ggtaagcacc ttctagcgcc tttgcagatc tataagaata
    24361 tttttcaact aattctaact ctctcttagt agggggaata actttatcat aaataccttc
    24421 taccaatata tgacctttgt tatcccttaa cgtattgagg gcttgaatta aataccatgt
    24481 cgaggaatca acaaccccac caaaggatga atgaatatca acatccgctg aacttactga
    24541 taaatcaaaa gtgacaatgc ctttatttcc accagcaatt tccaactgtc ctttaggatt
    24601 tttagggcca tcttcccaca cgatcaaatc agccccttgt agctgctctt gatacttttc
    24661 taaataataa tcaaggccta ctgaagctga ttcctctgct ccttccacaa taaaagtgat
    24721 atctagagga agttcccctt tgtgtcttga taaatatttt ttaactgctg ataaacgtgc
    24781 ggtaatgtga cctttatcat catctacacc acgtccatac atcttaccat atcgaagtga
    24841 aagtgtaaaa gggtcctcag tccactgttc tacctcatct gctggtacag tatcataatg
    24901 attgtaaaaa ataatacgct tagcatctac ttttgatgat ttgaaatttg ccacgataaa
    24961 gggagcgcta tagctgtcat ctataatgac ttctgcgcca gcttttatga aaacttctcc
    25021 aagatagctt gataagtcat ttaaaccaac tttctgagca aaaattgact taattgcaat
    25081 taaatctctt aaaatattga tatcatcctg aacaatagta tcagatagaa acttttgaat
    25141 ttgattttct tcgtttgtta aagtcatctt tcatccacct tccttcaaaa acaatccaag
    25201 agcctggcac attgtattcg tcccaggccc tttatggcta tatggctgat aagttgtatc
    25261 ctattaggat tccctgttaa acttatacag tcaattcacg ttaccattgt ggaatatcag
    25321 ctgaagtatc tttgataact tttttcactt catctgtgtg ataagcatcc aagatagctt
    25381 ggatagcttt agcgttcttt tgctttttcc aatttttacg tcccgcaatg atattaatcc
    25441 attgttttga atttttatct gatttctcaa caaagatagc atctgaaggt tttaaattag
    25501 cttgctcaat gtatgtatta ttaataatag ctgcatctac atctttgagt gcacgtggtg
    25561 tttgactcgc atctaactcc tgaatattaa tatccttttt attagatgtg atattagcaa
    25621 ctgttgcaac cttcttacca gaaacattca atttgattaa acctgctgac tgaaggacat
    25681 acaatgcacg gctaccattt gttgcatcat ttggaattgc aatagtggct ccttttttca
    25741 attttttaag agattttacc ttctcagaat agatacgaat tggagctaag taagtctttt
    25801 caagtggaat taagtttttc ttattttcct tattccagtt ttctaagaaa ttgtaatgtt
    25861 gaaaggcatt aatatccaca tccttattgg ctgtcgcttg atttggttgt gtataatctg
    25921 taaattctgt aaatttgatt ttagctttat cacctactag cttttcaatt ttatcccaac
    25981 gtgctttttc agtgtcagaa aaggtcataa caccaacttt aacaacttta cttgaagttg
    26041 agcttgctga aacttcttga ttactaaatg tgactcctaa aagtgttgtt aaggctaatc
    26101 ctaagacaat atgttttaac ttcataataa attccctcaa tttttgtgat aaagatatta
    26161 tcccatagga agttattaga atcaaattgt tataaactgt ctcttgttat agttttaaac
    26221 tatataacaa aaaactaagg attgacatct ccttagtttt tattactact tctccttagg
    26281 agtaacgaga tctttctcat ttggtacgta atcacctcca aggtattctt tagctatttt
    26341 tgaaagtgtc ccatctttac gtaattgttt aaggcgatta ttcacatact tttgcaagtc
    26401 tttttgatcc tgaccaaaga tgaaataaat ataaggctga tctctcatag tcaaagggat
    26461 ggtttttaaa ttcgtgagac cttgattctt tatgatagcg ttaacagttg gtccgtcaaa
    26521 aagtttaaaa tcagcttttc catcactcaa attcgttaga atctgtgtaa tattttcatt
    26581 agtatatttt agagtaacag gatttttctg atgctcttta ttgaaatttt ctaattgctt
    26641 ggcagttgtc gttccttgga caacctgtgt tttatgacca ctaatatcat tataagcttt
    26701 gatattacta ttcttaggaa ctgctaaaac tgaaggagta gagcctattg ggtatgaaaa
    26761 taaatatttt tgagatctct ctgatgaata agaaatatta tttccaccca tttgaaactt
    26821 tcctgaatca atgccggtaa ataccgatga ccattctgtt tttttaaaag tgactttata
    26881 gttatctgaa cctttaaaaa cagctttggc tacttcaata tcaaagccag tcagtttccc
    26941 atccttcaca taagaaaaag gtgccgttgt tccaacagtt gcaaaattaa cttctttttt
    27001 gcccgtctta gtagtatgag attttgagct acaagcagct agagacgcta ctgtcactaa
    27061 aataattcct gttacactta acactttttt tattgtcatc ttttttctcc tctttaattg
    27121 tacacgacat cataacatct ttaagttttt aactaacact tatcttctat cgtctgagag
    27181 tttttgctat tatatggatg ctacgtttca ttaacaaaat aaccaaataa ggtttggttt
    27241 tcaatttggt gaagcattaa ttctggatgc cattgaacac ctataataca ttgttctgtt
    27301 cgactttcat aagcttctac tgtcccatct ctaggatctc tagcactaac tatcagacct
    27361 tcagctaaca gtttaacact ttgtctatga agagaattga tcttaaaatc acctgatata
    27421 aagccttcta gaaaactccc tttttccaca tttaaataat gagcaaatcc aatgtaaggc
    27481 tcttgatagt gattatcaat agattgatta agtgtaccac caagcgccac atttactaac
    27541 tgcatgccac ggcaaatcgc aaagattggt ttattttgtt tcaacgcttc ttcgactaat
    27601 gcaaattcaa aaatatcacg cgcaagtgaa taatcatcac tctcaattat cttctcttca
    27661 ccataatagg atggtaaaac attttggcca ccagatatga tcagcttatc tatcatctca
    27721 acataagctt tggcactttc tgcttcactt attggtagga taactggtaa cccgcctgcg
    27781 tttttaactc cttccgaaat atgcctagat accgaatcat aataataacc tggaatatct
    27841 gacatttctc tttcatttcc tgtaatacca ataataggct ttgttaacat tttgctccct
    27901 catcaaccaa caactgtcta ataattatca ttttacacct aaaaaaactt tttgaataat
    27961 attttttacc tatcacttct ttaacaatac ctatcaactc tcacaaaaat aaaaaccata
    28021 gtattaatac tacggtttta ttttttagaa atctgctaca ttatgataaa ctttttgaac
    28081 atcatcatct gattcgagtg cgtcaataag cttttcaaaa gtttcaaggt catcaccttc
    28141 caatactact tctgattgag gaatcatttc aagttcagta acttggaatt cttctacacc
    28201 attatcgcga agtgcttgga tacctttatg aagatctgtt ggggcggtat aaactgttat
    28261 tgttccctct tctgcttcaa catcatctac gtctacatcc gcttcaagta attgttcgaa
    28321 gacagtgtca gcatcatcac cagcaaaaac gatgacacct tttttatcaa ataagtagga
    28381 taccgatcct gaagctccca tattgccacc gttcttaccg taagcagtac gtacatttgc
    28441 tgccgtacgg ttaacatttg atgtcaaagt atccacaata atcattgaac catttggacc
    28501 aaaaccttca tagcgtccct ctacgaaagt ttcatctgtg tttcctttgg ctttatcaat
    28561 cgctttatca ataacatgct ttggaacttg tgcttgctta gcacggtcca aaacgaattt
    28621 tagagctgag tttgactcgg ggtctggttc accttgcttt gcagcaacat atatttcaac
    28681 accgaattta gcgtatactt ttgagttagc accatcttta gcagtctttt tggcaacaat
    28741 attggcccac ttacgtccca ttggaaaatc tcctttgatt tcatttttaa taacctgctg
    28801 gctcatattg gcaagcaatc ttaattttaa ctctattatt atagcacttt gatagcaatt
    28861 ggtaaaatgg aaattctatc accaagctct agcatctgtc ttatgtttta agtaatcaat
    28921 ccatctacta tcctttacac gacacaaggt aactgataaa ccagccatat ctaaacttgt
    28981 aattaggtgg cctgttttaa taaaaggtaa ttgtaaatcg tctaaagcca gaaattccat
    29041 gacagcattt gtaaaaagta attcctccat tttagaactt cctccaagat tatttatcaa
    29101 tagaatgaat gcttctccat cttgccaacg taatttcatt ttcaatttgt taactagttc
    29161 gtttgccaaa tgttccatgg acacgaaagg cattgtccgg tatcctggct caccatgaat
    29221 accgattcca aatgagatgt aaccttcttc taaatcaaaa acaggttgat gctgacctaa
    29281 aatagtggct gacttacttg ctacacctaa agtagccatt gaagttgtca aagatagtcc
    29341 tagctgttct aattcatcta agcttgctcc ttctaaagca gcttgtccaa taattttatg
    29401 tagcaacaca gtaccagcaa cgcctcgatg acgaatttta aaatttgatg tttcaacaga
    29461 gatgtcatca tgagaaacga tgtatttgat tggaatgcct tcttgacgag cctgttcaat
    29521 cgcttgggaa aattcttcca aatcagcttc aaaatttttg ataataacaa agacaccttt
    29581 gcctcgattg ataaatctaa tagtctctag aatatcacta gcacatggtg gtacaaaaat
    29641 aggaccacta atcgctgccg atagcattcc ctcaccaaca tatccaaagt gagccggctc
    29701 atgtccgctg cctcctcctg ataaaatcgg aattacttgc tcggattgcc ctaaataatg
    29761 aatagcgggc aaatctttta tttgctctac tttaggatta gttttagtga ttgcttttat
    29821 tgtttcaaaa acatattcca ttttatgatg ttctgttttt ctcatatcct tgtcctaata
    29881 attttctatt gatgttctga taattttttc aatgactttc ctattaaata attcttcttt
    29941 tgagttattt attaactcat attttataaa ataagagagg gctttactat gataattaat
    30001 aaatgtaact ctttctttct gattaaagtt agatttacta taatcagaaa gaagcttatc
    30061 tactaactgc tcacagtaag actcaaaata cgaagaaaaa tcattttgat cttcaatttg
    30121 aaataattta atatagaatt cttgattcat tttaaaaaaa gtcaacaact ctgataacaa
    30181 ctgaaaacct gagatatagt ctaaattatc cgtaacttgt tcgcttaatt ctgtttggaa
    30241 aatccattct aataaagcat atttatcaac aaagtgatta taaaatgttt gacgtctaat
    30301 acctgcatct tccataatat cactcaccga tattttgtca aaagcttgcg aaataaataa
    30361 acgtttaaat gacttggcta tttttttctt agtaataagc gaagaagtca taatttctcc
    30421 tttattttat attgaattgc tgatgattta ttatggttta gtgtaacatg atttatgatt
    30481 attgaaaacc ctaacaaagc ggacaaacaa gaaaatctgt ccattccaga aaaggcttac
    30541 aaattttata ataaaagaga attaaacata aaaggagttt ccaaatgaaa aaaattctca
    30601 atcaaccaac tgatgttgtt actgaaatgc tagatggatt agcatatgtt cataatgatt
    30661 tagtacatcg catagaaggt tttgatatca ttgctcgcaa tgaagagaag tcaggaaaag
    30721 tggctctcat ttctggcgga ggaagtggac atgagccttc acatgctggt ttcgtaggcg
    30781 aaggaatgtt atctgcagct gtgtgtggtg cagtatttac ttctccaact cctgaccaag
    30841 ttcttgaagc aattaaagaa gctgatgagg gggctggagt tttcatggtt atcaaaaatt
    30901 attcagggga tatcatgaac tttgaaatgg cacaagatat ggctgaaatg gaaggcatag
    30961 aagttgcaag tgtggtcgtt gatgatgata ttgcagtcga agacagtctt tacacacaag
    31021 gaaaacgtgg tgtagctggt acgatactcg tccataaaat tctaggacat gctgctcgtc
    31081 atggcaaatc acttcaggaa atcaaagcta ttgctgatga attagtacca aacattcaca
    31141 ctgttggact tgcactaagc ggggctactg ttcctgaagt aggcaagcca ggatttgttc
    31201 ttgcagaaga tgaaattgaa tttggtatcg gtattcatgg tgaaccaggt tatcgtaaag
    31261 aaaaaatgca accttcaaag gctttagcga cagaattagt tgataagtta attgaatcgt
    31321 tcgatgctaa atctggcgaa aaatatggtg tccttattaa cggtatgggt gcaaccccac
    31381 ttatggaaca atatgtcttt gctaacgatg ttgccaaact ccttgaggat aaaggcattg
    31441 aagtgaatta caaaaaactt ggtaactata tgacttcaat tgatatggct ggtttatcac
    31501 taaccttgat taaactggaa aatcaagagt ggttagaagc acttaatagt gacgttacaa
    31561 ccattgcttg gtaatcattc ctgttaatta gaacaaaagg aaggaactct tatggaagta
    31621 aaaactgcaa tagaatggat gcacactttt aaccaaaaaa ttcaatccaa caaggactat
    31681 ttatccgaac tagatactcc aataggtgat ggcgaccatg gtggcaatat ggcgcgtggt
    31741 atgactgctg ttattgaaaa ccttgataac aatgaattta gtagtgctgc cgatgttttc
    31801 aaaaccgtgt ccatgcaact tttaagcaaa gtaggcggag catctggacc actctatggt
    31861 tctgcattta tgggaattac aaaagctgaa cagagtaaaa gtaccatttc agaggcactt
    31921 ggcgcaggac ttgaaatgat tcaaaaacgt ggtaaagctg aacttaatga aaaaacaatg
    31981 gttgatgttt ggcacggtgt tattgaggcg attgaaaaaa atgaattgac tgaagaccgt
    32041 atcgattcct tagtagatgc tacaaaaggg atgaaagcaa caaaaggacg cgcttcttat
    32101 gttggagaac gctcagtagg acacattgat ccaggatcat tttcttcagg attattattt
    32161 aaagcattac tagaagttgg aggtgtctaa tgtctgatat tggtattatt gttgtttctc
    32221 actctaaaaa tattgctcaa ggagttgttg atcttatttc agaggtagca aaggatgtct
    32281 caataactta cgtgggtgga acagaagatg gtgagattgg tacttctttt gatcaagttc
    32341 aacagattgt agagcaaaat gataaaaaga cgttattagc tttctttgat ttaggttcag
    32401 caaaaatgaa tttagagtta gtagctgatt tttcagaaaa aaatattatc attaactctg
    32461 tcccagtagt ggagggagct tacaccgctg ctgctcttct acaggcaggt gctgacctgg
    32521 atagtattca atcacaatta gctgaattaa ctatcaataa ataaggagat attatgactc
    32581 aatttcttgg ggaattttta ggaacattta ttttggttct tcttggggat ggcgtagttg
    32641 caggaaatgt gctcagtaaa accaaagaag aaggaactgg atggactgct attgtttttg
    32701 gctggggaat tgcttgtacc gttgctgttt atgtttctgg attattcagt cccgctcatc
    32761 taaatccagc agtcacatta gcaatggctt ctattggagc aatttcttgg ggacaagtta
    32821 tcccctttat cattgcacag atgttaggag caatggttgc ggcaactatt ttatggttac
    32881 actattatcc tcattggaaa gaaacaaaag atagtggact tatccttgcc agcttctcaa
    32941 ccggacctgc tatccgccat acaccatcga atctccttgg tgaaattatc ggtactgcta
    33001 ttcttgtgat tacaattatg gcaattggtc ctagtaaagt tgcagcaggt cttggtccaa
    33061 ttatcgttgg tatagttatt ttcgcagtag gattctctct tggtccaaca actggttatg
    33121 ctattaaccc tgcacgtgac ctagggcctc gtcttatgca tgctatctta cctattgaaa
    33181 ataagggtaa ctcagattgg tcatacgctt ggattccagt tgtaggtcca attatcggcg
    33241 gtgtccttgg ggctatctta tacaatattg ctttgacttt agtttaacta aaaaaccaga
    33301 aagttaatac tttctggttt ttcatagttg cagtatgctt tcaaaataga aaaaacacca
    33361 aaatacttag tgttctttct ggagatttta tgaaaaaagt ttaggatgtt tatagtataa
    33421 ccgcccttac ttaacgtttc cttaaaagct cggttattaa cattacttcc taaattacta
    33481 tttatattct ccaagaaatg actactatta agctaaaaag cgctattgac atagcgcttt
    33541 ttagtctagt attatttagt ttcagtagta actccatcaa aatcttcttc attttcttca
    33601 gcttcttctc ttgtggcacg gataatacca tctttgtgat gagctggact gtaaattgta
    33661 tagagtttta acatctcagt ttcagaagtg ttaattacgt tatgttcagc acctgccgga
    33721 actattatag cgtctccatc tgcaacttca tattcattac catcaatgac aaccttacca
    33781 tgaccagctt caaaacggaa aaactgatca ttttctgcat gaatctcgct accaatttct
    33841 ccaccaacag gcagtgtcat taaaacgagt tgacaatgtt ctgcggtata gaggacttgg
    33901 cggaaatttg tatttcctag tgtcaatttt tcgatattcc catgaaatcc tttcataacg
    33961 atacctacct ttctatgcct acagtctact ctttttgctc cttactgtca aaaatttaga
    34021 taagagtctg acatttttaa taatatctac tgttccttga ttcctatcat ttcattgcaa
    34081 acctaaaata tagcctaatg acaatagcta tctatagctt tagaaacaaa taatgctgtt
    34141 ccctggcctc cccaattatc aatagacttt tggaaacgct catcttggat gtacattatt
    34201 ccaagacttg ctaataaatc gttagtgcag ttgtaaaact gagctgtgat ataatcttgt
    34261 aaaatctgaa cttgtttttg aactttctca tctgtcggag aaagttcttt caactgtcca
    34321 aagtctttaa aaatctgact catagcttgt gaaatcattg aaaaatcatt cttactatgg
    34381 ctattttcaa attctttgta actatctgta tccccccagc gtgttctagc ttcctcttga
    34441 aatgcttcta attcttcttg attataagct gtaaaatctg acatattttt acccctctgg
    34501 atagactttg catgttcaat tacttcttct agtcgctgtt tctttagttc cagccaccta
    34561 atttgatcaa gaagtgcttg attacgatca taatttggac tatccattat ctcttttatt
    34621 tttttaagag gaaattccag ttccttaaat aacaaaattt cttgtaactt aatcagtgat
    34681 tcatcatcat agtaacgata accattctct cctacaaatg aaggagataa caaatctatt
    34741 tcatcataat aatgaagtgt gcgcacacta ataccagaaa tgtgacttat ttctttaact
    34801 gttttcataa taacctctct gtaaagagta taatgtataa cgttaagtaa gggtcaaggg
    34861 aaaaatatta ttttgtgatg attaatttat aaagcttaaa tcaaaggtca ctttgactaa
    34921 caggttgata aaaagggata cgtccccatg tttgctctgc tatattagca tttcccatct
    34981 gataaacaga atctgctcgt tgcgtcaaac tatcccacgt ttgtgaacca atcctcgtca
    35041 cgatatccac agacttcttc acagacttta aatgctgttg acctttttgt gtaaaaccta
    35101 agacatgaat agcatttggt ataggacttt ccacagcatt tatcaagata taggttaata
    35161 aacgtcgaat tcttgctttg gtataccgtt tagttgcgac tttatcaact aactcatcca
    35221 cagtctccac ataacgaatt tgacttttta tacgattggc tatctcttca ttcacttgat
    35281 aaatttgtgt caaatcagaa tgcgtcatta tctgatattt gagtaaacta aaataatctt
    35341 gccaaactac ttgtggacta ttcaaaaaca agtcactgtt aggcatacct ttttctacaa
    35401 agaaacgatc agattgatgt ttacgaagac ttgttgcaga agcaaatatc tctgtttttt
    35461 ctgatgaatg ataaccagct ccttgacgtt gtatcggatt taatcgaata ccattttggc
    35521 tagctgcttt ggtgtaggct aagcctaaaa tgtgatttgg ggtttgtccc gaaaatgtaa
    35581 tccctgcaaa ttcttgccac atagcttgca ttttttgtgg ataggaaaga gaatccgatt
    35641 gttttttcaa aaaagcttcc atctcctctt ttttattcac ataaatatcc ccaatacggg
    35701 cataatcaag catttcttcg gtaccgaagc ataagttatc cacacctaat cgtgccagga
    35761 tagacacagc tccactagcg aaataatccg ctgactgtac cgaaaccaga aaagggagtt
    35821 caatcactaa atcagcacca ttttctagtg ccatctggga acgggtccat ttatctacaa
    35881 tcgccggctc accacgttgc ataaaattac ccgacatggc tattactttt atgccttgag
    35941 cttgttctaa aaggtactta tgcccattat gaaagggatt aaattcggca acaattcctg
    36001 taactgtcat acttctttct ctttcaattg attatttaaa gtaacagcat caataatatc
    36061 taggtgataa gttttacgaa ggtatatgag acgatctgtt tctttttcaa atggttttaa
    36121 tgccgataat aaatccaaat gaagtttagg gttcatcaat aattccaaaa taactttaga
    36181 ctttttctga cttacccgac gttcaataat ataaagtatc ccaaatatca tcagacaagc
    36241 gtataagaat attatcatct ttttacctcc aaattattac ttatttatga gctacaaaaa
    36301 accagcgagc agaagttgca gttggttttt tatcttcaaa atctgcatac accttaacat
    36361 ctttaaaacc tgcctgttca agtaaaatat cataagtcaa aatatcataa gtacgctctt
    36421 catgaacttc atcatgacgt gtaaatcgac catcttcttc ttgaacaaaa aatgttaatt
    36481 catggacaat agaatgaggt gcatcatctt cataagtatc ccacaccata gcaaagtcat
    36541 ctgcattttc atgataagaa taaccaggaa atactttatc tgtttgataa gtcgaatgaa
    36601 catcaaaaat aaagacgcca ttttcttcta aggccttata gacttcgata aaaacatcac
    36661 caacctcgac ttcatcttgc atataacaaa tagagtctga atagcacgtg atgagatcat
    36721 atttaccaac attagataaa tctaacatat ttccttcaat aaactggata ctttggtgag
    36781 cagaagttgc acgttttttg gcaagtttca gcatatcgcc actaagatca agtcctgtaa
    36841 cagcataacc agcttgtgca aatcgaacag actgaattcc cgtaccacat gctaattcca
    36901 aaagtttttt cttacctttt ggaaaatgac gaagtgaaaa atcagtccat ttagcatata
    36961 aagtatcatc catgacagca tcataaacag ctgcaaaagt ttcatacgtt gccatataat
    37021 agttctcaaa tctttcttga tattacatct ctcaaaaagc agtcccaaga ctgcttagct
    37081 ttattctgtc ataaagactt cagcatcaag caatggtgct tcatgccaaa gtttttcaag
    37141 gttatagtgc tgtctctcat cttcagagaa gatatgaacc acgacgctat tgaggtcaag
    37201 aagtacccaa ccagctttac tatcgccttc aaggtggcta gcatccccac catttccttt
    37261 tacttgttca cggatattgt ctgcgattgc ttctaactgc ctactattac ttgcactcat
    37321 aatcacaaag taatctgcaa cactcgttac aggctgtaaa tctaaaatta caatatcttc
    37381 tgctcgtttt tcatccgcag ccttaacgac tagctgtagt aagtcttttt ctgtcatttt
    37441 attcctcgtt tattaatttc aataagctat cttttgctac cattgtcgca aaacgatgat
    37501 gccaaatatt ctcgtaatgt tttttaatag aatccccact taaagttggt gtatcaaatg
    37561 ttaggtgtcc accttgtgga ataaagatat cataaccata ttcaaaagca acttttactg
    37621 atgtatcaaa acagtattct gtttgaagtc ccatcatgat taattgacta atatgcttac
    37681 tttgcaagta atcatgtaaa tgagtatctt taaaacaact attgtaagtt ttatcaatta
    37741 ttttgtgatg cgtcacaaga tgtcgctgtt cccaaatttc ccactgtgga gaaccttgag
    37801 gcaattcctt atcatgatgc cttatccata tcacttcaca attagaacct ttggcactta
    37861 aaagtaaatc atctaaaagc actgcaaaat ttgttaaatg ctttggtttc ttatccataa
    37921 taccttgttg gatatcaatt agtaaaagtg cacaagtcat actttcctcc aaaatgacca
    37981 ctgcatcgcc aaacctatca tgaccattat tcaataaaag aattagtcga gataaggtat
    38041 ataagcatta taggtttcaa tcgttttagg atagataggc tgagccttac ttgctaaaaa
    38101 cgcaactgtt ctagcagtct cataagctac tgctttatta aggtcaactt tcgctaactc
    38161 acgagcttct tcaactcctg gaaaatctcg attgtgttca atgtaatctg ctacataaac
    38221 gattttatcc aatgtggaca tttgggctga accaacagta tgttttgcaa tagcagctaa
    38281 aatatcttga tctttgatag ctagatcctc ttgtatttta taaatgccaa caagaccatg
    38341 ccaaatatta ttaccccatt tttttaggtc gggatctggt tggtatttat caattaatct
    38401 aagaaattca tcatcagata gttccttagc ataatcatgt aataaagcag ctagacccgc
    38461 tttttccttg tcataaccat accgttctgc taactctatt gcagcacgct ctactcctaa
    38521 cacatgatta aaacgtttgt cggacatcat atgacgcact ttactcaaaa gttcagtccg
    38581 atctaaacct gtgtaatctt tgtaggtcat ttgtaaagac cttctttcct gatataatct
    38641 aatacctctc taggcagtaa atagttgggc tgacgattac ttttaataaa ttgacgaatc
    38701 atacttgatg agatatccat caatggcaga tccacccaaa taacgggata tgatgtacca
    38761 gctttatatt ttggacgttg aacaccaaca aactgcacca ttttaacaag ttcatcaata
    38821 cgatgccact taggtagata ttccaccata tctgccccaa taatgaaata ataatcaaca
    38881 tctggatttt tttcaattag aagcttcatc gtatcgtatg tatagctgat acctttacgt
    38941 tcaatttcaa ttggttcaat agataaacca tcaatgcctt ctattgctaa ttcaagcatc
    39001 ttgagacgat gctgttcatc aattgtctct tttttatcaa tatgtggtgg ctgaaattca
    39061 ggcatcaaca gaacttggtc taaacacaat tgctgacgta cttgatctgc aacaacaagg
    39121 tgggcgttat gaacagggtt aaaatttcct cccataattc caatttgttt tcggttagta
    39181 tctcgctttt tctcttctaa ttcaactttt gtaaatggtg ttagcaattc gagtgccata
    39241 attcctccat gaacaagtag tatctctaaa aaaacaaaca ctgattatca tgatcagcta
    39301 tcatacggaa gtgtaactta ggtaaatcgt tttttagttc tatactctat ttcttaatct
    39361 attgtagttt agtttgattt tactgctttt actttaactg aaattttacg attttctttg
    39421 cgcgctgact ctttataaag aatcaagata cgtcctattt taaggacagt atcgcagcca
    39481 atttcatctt ccagaacttc agcgacatcg tggatatctt catcggtatt ttgtaaaagg
    39541 gttactttga ttagctcacg tgcatcgagt gcattacgta cgcttgtttt gatttgatca
    39601 tttaaaccat ttttcccaat ttgaataatg ggtttcatag aatgtgcttc tgattttaag
    39661 aaagcacgtt gtttagatgt taacatatta gtttcctttt tatctattat aggattatga
    39721 ctgtatcatc agctaaatta gatgaaaaac cgtcatatgt tgctggttaa tttagttatt
    39781 aaattaaagc tttgcgtaaa acaactgcca cgccttctgg agcccaagca gctactacta
    39841 ctggattttc tgcttctgat ttcacacgta tccaaccaag acccgaataa acaatatcca
    39901 ttttatcttt gattgtaaat tcatgacgaa ctaatttagg aaaatctgaa acttccttac
    39961 ctgttggtgg tgtcaataat tttccaacat gtttagtgta aaattcatct gctccaacta
    40021 gtttagtgcg gtgtaaattc aggttattat caaaatatgc cgtaaaacct tgtttttgac
    40081 cagaaataaa atcaaaacgt gctaggccag ccaagaataa tgtttgttct gaattaagct
    40141 gataagtttt gggttttatt tcttttttag gactaacata tttaagattt tttgctgtca
    40201 aataatgagc catttgatga cgatgaataa tacccggggt atcaaaaata tagctaccat
    40261 catcaagagg tatttctatt ttatctaacg ttgtacctgg gaaacgtgag gtcgtaataa
    40321 catcacgact accagtaatt tcacgaataa tagcgttaat aagagttgac tttccaacat
    40381 tagtaacccc aacaacataa acatcttgac catgacgata cttttcaatc gtatcaatca
    40441 aatccttaat agcatgatga ttttgggcac ttgtcaagat gacatcaaca ggacgaagcc
    40501 cctcttcatg agctctctca gttaaccatt gtgttacttt tccatcttta accgatttgg
    40561 gaagaatatc ttttttattt cctactaaaa gaacatcgtt tcctgcaacg aaacgactaa
    40621 gaccaggaat aattgaacca ttgaaatcaa aaatatcaat gacattcacc acaagggcat
    40681 cactatctcc aacctcatgt agtaatttca aaaattcatc atcagtgata tgaacgtcag
    40741 taatctcatt atagtggcgc aatctgaaac aacgctgaca gtataattct cctgtctcta
    40801 aacctttttc aagtgctgca cgaggtgtat agccagcagc atctttattt tcagtttgaa
    40861 tcctggctcc gcagccaata caaaataact cttccattaa attccttttt gatatacgat
    40921 tttaccgtaa ttctcttcta tttttttcca aacgcgacgt tcacgtaagc gattgaactt
    40981 ggtattccaa gcatcagatt taacaattgg ttttaccaat acggacttta ttcctgcgcg
    41041 atgactagca cgaatatctg tcatcaattg atctccaacc ataatcacct cgtcacgatc
    41101 aaagccataa cgctcaattg ccatgtttat accacgagta aatggtttca tcgcacgact
    41161 tacaaaatcc acaccaaaac gtgatactgc acgttctacg cgagcatgat tgttattaga
    41221 cactactaca actgaaatat ccgctgttgt catctcatct aaccatgcac gcacctcagc
    41281 agtaccatct ggattattcc aagcaatgag tgtattatct aaatcaacta aaaccgcacg
    41341 aatgccatga cgtaataaat cttctgctct taaatcataa actgcctcaa ccacgaaggt
    41401 cggcctataa tcatctatac tcaatttcat ctccaaaaaa tatattttac tattcattct
    41461 accataattc ttaagagata gcgagtaaag aaaaaaggtc tttaaagacc tttaaattag
    41521 ggtttcaacc ccaaaagata ctatcttact cgtttcattt tcttcaatgc aattaaatct
    41581 ttgtaagaga ttagagttac agcaagaaaa ataaacagca tcccaagtaa atctattgga
    41641 taaaaaattt ctcctaaaac aagtactgtc aaaaataccg aagaaactgg ctctaccgat
    41701 gctaataagc tacccttgac agcacctaca atactaaccc ctttaaggaa aaaagtgtaa
    41761 gcaaaaatag tcccaatacc aataatgcca atataagcaa gtatactcat cacattaatt
    41821 tgtaaggggt attgccacgc ttttgtcacc aaactaaata aaatcccacc tataaacatt
    41881 cctgaaccaa taacaatcgt acttccccat tcatgaataa gtttcacagg taataaaata
    41941 taaatagaat aagtaatggc ggaaccaagc ccccacatta atcctttcgg tgtgatagct
    42001 aaacttccta acttcccatg cgttgcaatc atataggttc ctaaaattgc catagcaatt
    42061 gctattattt caaaagatgt tggtctttgt ctgtttagga tacaaacaat actcaaaata
    42121 agtattggtg ccatatattg caaaaccgtt gcagttccag catttgttaa atgaatcgct
    42181 ctgagaaaag catattgatt taaaactaaa ccaaatatag aaaaaagtaa aacctgcttt
    42241 aaaaattttg gctgtttcca cgctgctact aaatgctcct tttgttttga tctagctaaa
    42301 gacaagagaa agatacctgt tatcaacaat cgtaaagaag tcaaaaggtt aacgtgtact
    42361 ccgtgcgaca tcaagtattg accactaatt ccagaaatgc cccatgctag tccggcagct
    42421 aaggtcatca ttgttccttt ttctttttta gtcatttttc ccctcatttt tatttcttaa
    42481 acactgtagt taaagtcatc tgataaatat cttagcttct taattattta tccctaggtg
    42541 ttcgtgctaa attagcgaat aaaaaccagc caaaaggctg atttctattc ttcttttaat
    42601 tttgctaatt cttgcgctaa cagcttcaga gcaacttgtg gattagcttg tccttttgtt
    42661 gctttcatta gataacctgt gaaggcctta tcagcattgc gttttcctga cttaaagtca
    42721 atcacagcag cttcattatc tgcaaagact tgatgaataa ttgggattaa tacttcagga
    42781 tctgaaattt gaactaaacc agccttttta acgaattctt cagctgaacc accattttta
    42841 gctaaatgca caaaaacttt tttagcaatt ttggatgaaa ttgtaccgtc agcaatcaag
    42901 ccaatcatct caaccaaatt ctcaggagtc agaccaattt cttcgattga ttttgattca
    42961 ctgttaagaa attgcgccac ttctccttgt aaccaattgg aaacttgttt tgcatcaccc
    43021 ccaatagcaa cagctttttc aaagaaatca gaagttgctt ttgtagctgt taattggcta
    43081 gcatcataag atgatagacc aaaagacgat acatatttcg cacgacgttc ctgtggaaat
    43141 tctggtaatt ctaaacgaac ttgatcaatc cattcatccg agatatcaaa tagtggtaaa
    43201 tctggttcag ggaaatagcg ataatctgat gagccttcct tgacacgcat caaaatagtc
    43261 tcacctgttg tttcatcaaa acgacgagtc tcttgctgaa tttgaccacc agatcgtaaa
    43321 acttgtgctt gacgtttttc ttcatgtatc aaacctttac gtacattatt gaatgagtta
    43381 aggttcttaa gttctgcttt agtaccaaac tcttcttgac cataaggacg aagtgaaata
    43441 ttggcatcta cacgcataga accttcttcc attttaacgt cagaaattcc agtatattgg
    43501 ataatctctt tgagagctgt caaataagca taagcttcct caggagaacg catatcagct
    43561 tctgatacaa tttcaatcag tggaacacct tgtctattaa ggtcaacata ggaataacca
    43621 tctgtcccat gtgtattttt tcctgcatct tcttcgaggt gggcacgctc aatacgaatt
    43681 ttttttcgtg taccatcttc taattcgatt tcaatccaac cattgtaacc aataggctca
    43741 tcaaactgtg aaatttgata agctttagga ttatctggat agaaataatt tttacgatca
    43801 aaatgcatgt tttgatggat atccatattc aaggcaagtg ccgccttgat tcctgcatca
    43861 ataactcctt tattcatgac tggaaggaca cctgggaaag accagtcaat tacatttgta
    43921 ttagcatttt gttcctgacc aaaatgagcg gaagaaggtg agaaaatttt agagttcgta
    43981 tttaattcaa catggacttc aagtccaatg actgtttcaa aattcattag ttctcacctc
    44041 caaaaatttt aggttgttgt ttatgataat ctgtcgttgc ttcgaaggca gcagcaacct
    44101 gatatattgt ttcttcactg aatttcggac caatcaactg cataccaacc ggtagacctt
    44161 gatcaaaacc agcagggatt gaaatacctg gtaaaccagc caaattaact ggtattgtta
    44221 gaatatcagc taggtacatt gcaactggat cgtgatttaa agaatctaag tcaaaggctg
    44281 ttgttggagc tgttggacct aaaatcaagt cataatctgc gaaaactttt tcaaaatctt
    44341 gaataatcaa actacgaact tgacctgctt ttttataata agcatcgtaa tacccagaag
    44401 ataaactgaa cgttcccagc ataatgcgac gcttaacttc gtctccaaaa ccttcagatc
    44461 gtgtattgac atagatatca tccaagtttt tataattttc tgtacggtat ccataacgaa
    44521 taccatcaaa acgttgtaag tttgaagaag cttccgatga agcaacaata taataaacag
    44581 caacaccgta tttagaatgc ggtaaactaa cttcctcaat aactgcacct agtttttcca
    44641 gatgttttgc ggccttaata atcgtttctt taactccttg cgctattcct tctcccaaat
    44701 attcttttgg taaagcaatt ttcattcctt gaatatcttg accaatttta gcagtaaaat
    44761 cgcctacagt acgctcagaa gaagttgaat ctcttacatc atgcccagaa ataactgtca
    44821 aaagctgtgc attctctttt actgtttgag acatcggtcc aatctggtca agcgaactac
    44881 cgaaagcaaa caaaccaaag cgtgatacac gtccgtaagt tggcttcata ccaacgattc
    44941 cattaaatga agccggttgg cgaattgaac cacctgtatc agagcccaat gataaacgta
    45001 cttgtcctga agctactgct gctgcagaac cacctgatga tcctcccgga actttactat
    45061 ggtcccaagc gttatttgtc tttttaaaat aagatgtttc tgtagagcct cccatggcaa
    45121 attcatccat gtttgcttta ccaatcacaa tcatatcttt agcatataac ttttcaacag
    45181 ccgtagcatc aaagattggc tcatagttat agagcatttt agaagcagct gttgtaagaa
    45241 tcccctttgt tgaaatatta tctttaactg ctaagggaat acctgacata acattatcag
    45301 catcaattcc tttatcatca atttctttgg cttgagctat tgccatctca tcagaaatag
    45361 taataaaaga tccaaccgct tgttcacgcg catgaatatc ttccaatgta gcttttgtta
    45421 attcagttgc tgaaatttct tttttaacga gaaaatcatg caactgatca attgattgat
    45481 tattaaacga cattaggcat ctcctccatc ctcaagaatt gcaggtactt taatataata
    45541 atcttgatgc tgtggtacat ttttaaataa gtcatcacga ttatggcctg gttgagcaat
    45601 atcttcacgc atcacggttt tgcgatctgc catggtcgtt gttactggta cgccttctgt
    45661 gtcaacttca tttaagagtt caatcatatc aacaatcttt gacaaacttg acgcaaattc
    45721 tttagtctct tgatcagaaa agcgcaattt tgacagattg gcaacatgac gcacttcttc
    45781 ttcagaaatt ttcatgagtt ctccttatct ctgtatcatt ttcgttttaa gatagtctct
    45841 actattatag catattaaac tctcaatgtt atcacattat cccagctttt cataataaac
    45901 gcttcttacc tatgataaga agcgtttact gtttatttat ctattgttat atcatttctg
    45961 ctttgtttga ttttagcctg tgcagccgct aatttagcaa taggaatcct aaacggcgaa
    46021 cacgatacat aatgtaaccc cagttggtaa cagaactcaa tagatgatgg ttctccacca
    46081 tgctctccac agatacctat tttaaggttt ggcttaactt cttttcctaa cctcactgcc
    46141 tgcccaataa accttcctat tcctttttga tctaaaactt ggaaagggtc tttttttaga
    46201 agtcctttat caacgtattc tcctaagaat tttccagcat catctcgtga aaatccaaag
    46261 ctcatttgtg ttaaatcatt ggtcccaaaa ctaaagaaat ccgcaaactt tgcaatatca
    46321 tccgctatca tacaagctct tggtgtttca atcattgtcc caatagggaa tgataagtta
    46381 atttttttag atttactctc ctcaacaatt gtctcttcaa tcagatcacg tattatagag
    46441 atttcttcat gcgctgtcac taaaggaatc ataatttctg gctttgcctc atatccctct
    46501 ttcattgcaa gtatagcgcc ttgtacaaga gctctcacct gcatttggta aatttccgga
    46561 taggtaatcg caagtctaca accacgatga cctaacatag ggttgaattc ttctaatgtt
    46621 tgaatccgtt tttcaatagc taaagtagat atccccattt gtcttgccat agattctata
    46681 gactctttat catggggtaa gaattcatgt aaaggagggt caagcaaacg aatcgtacaa
    46741 gctttacctt tcaatacttt aaaaatttga taaaaatcgt ctctttggaa agataaaagc
    46801 ctttctagag ccttccttcg gctcaatatt tcatccgcta aaatcatttc tctaacgact
    46861 ggaatacgtt catcgtcaaa aaacatatgt tccgtgcgac aaagacctat tccttctgct
    46921 ccaaagtcca aggccttttg agcatctctc ggattatctg catttgaaca caccatcata
    46981 tctcgatttt catctaccca tttcataaat atctcaaaat cacgatctac gctagtctct
    47041 gccatcggaa cttgtcctat ataaacattt cctgtagcac catcgattga taacatctca
    47101 ccttctttta tcgtttgatg cccaatagaa atttctcgtc ttacttcatc aaccaataat
    47161 tgactacagc ctgcaacgca tggtttcccc attcctctgg ctactactgc cgcatgggaa
    47221 gtcattccgc ctcttgctgt tataatcccg tttgcactaa ccatcccctc gatatcttca
    47281 ggtgatgtct cttgtctgac caatagcact ggatttccct ttttcatctc tttaacaaca
    47341 tcttctgcat ggaaataaac tttcccataa gcagctccag gagaagctgg aagtcctgtc
    47401 gtcaaaataa tagctttctt taaacttttc aagtcaaaag aagggtgtaa taactgatct
    47461 aattgtttgg gctcgatacg tagtatagct tcttctttac taattaggcc ttcatttact
    47521 tgatctactg ctattttgat agctgccttc gctgtacgct taccgcttct cgtctgaagc
    47581 atataaagat taccattttc aattgtaaac tccatatctt gcatatcgcg ataatgagct
    47641 tctaactttt gggtaacgct tacaaactct tggtaaataa taggcatttg ttctgccaat
    47701 tttgatatgg attgtggtgt tctgatacct gcaacaacat cttctccttg tgcattaatc
    47761 aaatactcac caaataaatt tgctgctcca gttgatgggt ttcgtgtaaa agcaactccc
    47821 gttccgctgt tatttcccat attcccaaaa accatagctt ggatattaac ggctgtccca
    47881 agcgtctccg gaatatcatt taatttacga taaatcttag ctcttggatt attccatgat
    47941 ttaaaaactg cctcaattgc taacaaaagc tgccttttag gatcttgcgg aaatttttct
    48001 cctgcttctt tttgatatag ttccttgtaa aattcaacaa gtctctttaa atctgaacca
    48061 gttagttctg tgtcatcttg ataacattta tctgtcttaa gtcgatctaa aatggtatca
    48121 aatttatatt ttggaatgcc tgtaaccaca tctgcaaaca tttggataaa acgacgataa
    48181 ctgtcataag caaaacgctc gtcttctgtc tttttcacta aaccttggac actacgatca
    48241 tttaacccta aatttaaaac tgtgtccatc ataccaggca ttgaaaaaac tgatcctgac
    48301 cgaactgaca ccaacaaagg atcatcaaca cttcctaatt gcttgttctg ttcaacctcc
    48361 aattgtgcca ttgcttgatc aatttgacta agaatactct ctcggatatg gcaagcgtta
    48421 tcataataat cattacaagc ctgtgttgta atagtaaaac cttgtggtac cggtaaccca
    48481 atactcgtca tttcagctag attagccccc ttaccaccta gcagttcctt catttcctta
    48541 caaccttcat caaaatgata cacaaactta gtttccatca gcgtctcctt ttacagattt
    48601 ttagctactt gtccatttgt ttttaagatt tctaaaataa tcgttgctgt ttcttcgatt
    48661 gctttatcag atacattaat gattgggcaa cctaaatttt tcataacttc ttcagcatac
    48721 ctcgtctcct ccaaaatccg ttctaaactg gcataatttg ccgtactaga aagacctaag
    48781 gactttaatc gaacttttcg gacttgatta agatgatcaa ccgaattagt tagtccgata
    48841 atccttctag aatcaatcat acgtaattct ttaggaacag gaacctctgg aaccaaagga
    48901 atattaataa ctttaatatt tttatctgct aaaaacatac ttaatggtgt tttagaagtt
    48961 cttgaaattc ctataataac taaatctgct tgtaaaatac ctcgcggatc tctcccatca
    49021 tcatatttta ctgcaaattc tattgattct actcttttga aataatcatt gtctaagcgt
    49081 cgtaaaatcc ctggttctcc caaaggatct atacccgata tccttgatat tccttgaata
    49141 acatttgtta ataagtcaac ataagcataa tgttcacttt cacatcgttt ctgtgcatag
    49201 gaagccaaag caacatcaac taaactaaac atcataaata ctgtcttctg gctagcctct
    49261 tcaaaaactt gttctaagcg ttcctgactg ttaatatagg aaaaacgttg aaaatgccaa
    49321 tcatcatgtc ctggaaattg tgacaaacat gctttcgcaa tagctttggc tgtttcccct
    49381 aaagaatctg aaataataaa tatagtgagt tgatcttcca tcagtttcct cctaataatc
    49441 tcttatttct aaaaacaaac tagcgaggat tgttttagat aatttcccga tcactttaaa
    49501 tttttcggga tattgcttat catgacgaac gacagggaga ctatccactt ttctgctcac
    49561 taatttatca gctgccgcaa aaagactttc attttctaaa acagttgtca cgtgtggcat
    49621 acgtgtcatc accattccta ttggcatttt agaaagatct cctccgccaa tactgatttt
    49681 tagtaaatct ttacgtgaca cgactccaca gagaaaatca tcatcatcca agataaaagc
    49741 acaaccagca tcttccataa aaatatgtac aataacatca taaactgaat ctttttgatg
    49801 aactgtcaga gggatcccca taatttctga aactgtcatc ttttcaaaat gacttgtccc
    49861 tattgaagca tgatactgtc ctaaataaaa ataaccaacc ttaggttttg catctatgat
    49921 acctagcata cttaaaaccg ccaaatcaga ccttagtgct gctcttgtta ctcctaattt
    49981 ctcagctatt agctcaccag taatgggctg aaactgtttg ataatatcaa taattctttc
    50041 ttgcctttga tttaattgaa taagatcacc ttctttatat attgtatact atttatttta
    50101 tatgttatat tatatatctt tttttactga aatcaagtaa aaaaagatac tattttaaaa
    50161 ataatcatac tatatatttg cttttagaca tatcttttgt taaactttct tcgaaaacgt
    50221 tttcgaatat aagtatcgga ggttgtaata tgacaattaa aaatctcact gttgcaggaa
    50281 gtggcgttct aggtagtcaa atcgcctttc aagcagctta caaaggaatg tctgtcacca
    50341 tatatgacat taacgatgaa gctttaaata agggaaaaga acgcatcaaa aaattagcta
    50401 aagtctatca atcagaaatt gaaacagcaa aagaagcata tagtgataaa gctaaaagta
    50461 tcaagtataa taaaaactta ttgcctagct tagaccatat ttttctttca aaagttgccg
    50521 attcattaga cttgattgct gatttaccaa accaaattac tttttcaaaa aacttagacc
    50581 aagctgtaag tgacgccgat ttagtaatcg aagctgtccc agaaacagtt agtataaaag
    50641 aagattttta taaacaatta gctaaagtgg caccttcaaa gacaatcttt gcaacaaatt
    50701 cttcaacact tgttccaagt cagtttgctg atataacagg acggcctgac aaatttttag
    50761 caatgcattt cgctaacaat atttggcaaa ataatattgt tgaaattatg ggacataagg
    50821 gaactgacga cgaggttatc aaagaagctt tggcattctc aaaagatatt ggtatggtgc
    50881 ctttacatat tcataaagaa caacctggct atattcttaa ttcaatacta gttccattct
    50941 tagagagtgc actagcattg tactatgata aggtttctga ttctgaaact attgataaaa
    51001 cctggaagtt aggaacagga gcaccgatgg gacctttaga aattttagat attattggca
    51061 tcgataccgc ttataatatc atgaagaatt attcagatac caacagcgat cctaatagtc
    51121 ttcatgctca ccttgcaaaa atgcttaaag aagaattcat tgataaagga cgtaccggta
    51181 aagctgctgg acacggcttt tatgattacg actaaactat aaaagaggtt agataaaaat
    51241 ctaacctctt ttataattca actaaagaat agttgcacca agaacagttt taaaatgatt
    51301 taaagcccat tgatgattac tatcatttaa gctagctaca gctgctgctg ggacctcaat
    51361 tttataacct aagttataag catcaattgc tgtgtgtaat acacaaatat ctgtcaatac
    51421 tcctgtcaat atcaaagtat ctactcgtct ttcacgtagg cgaatatcta aatcagtgcc
    51481 agaaaaggca gaataatgac gcttatctat ccaaaaaaca cgactatctt ccttaatagt
    51541 ttcatataga gttcccaaag gaccatatag gtttctacca gaagtccctt taatattatg
    51601 ctcaggaaaa agattgctct ctggatgaaa aacatctcca atatcatgat tatcaatcgc
    51661 aaaaaaaata taatcccctg accggtatgc tttctctgta acgtcagcta tagctgaagc
    51721 gatagattgc gctggtttac ctgccgttaa tttcccatca tctgccacaa aatcatatgt
    51781 gtagtctatt gagatgagtg cttttgtcat ttttcttctt atctcctctt gatttcaaaa
    51841 aaggaactga aagctactcc cagtcccatg ctatgattaa ttatattctt ttaatttgtc
    51901 aaaaatacct tcgttaataa ctttgaggta tgttcccttc ataccgagtg agcggctctc
    51961 aatgattccc gcagactcca atttacgaag agcatttaca ataacggaac gtgtaattcc
    52021 aatacggtct gcaataacag atgccgttag gcgtccttca agtccatcta attctcctaa
    52081 gatagcagct acagctttca tttctgaata agatagagtg ttgatagcca ttgtcaccgc
    52141 tgtttgttta cgaatatttt cttctagatt ttctgtttga aggttaagta attgaatacc
    52201 aacaacggtg cttgcaattt caactaaaat caaatcatcg tcgctaaatt ctttatcatt
    52261 acgccagata ataaatgttc ccaagcgcat tccaccacca taaattggag cgattgttgt
    52321 aataccatct tggaagtttt cctttgtctc aactgggaaa attgacaaat cattatctac
    52381 tgataagtta gcttctgtat cataaacacg actagcagat ttaacatagt aatctgggaa
    52441 ttgttttgtt tcaaaaaatt cttccacacg gtcagtgttt gttttatatt tcatggcata
    52501 gccaagtaag ttaccaccac cattaataat acatgcgtta cagtcaataa tatcagctaa
    52561 ttgagccgcc attgtattat atggtagttc ggcatctaag ctatctacgg aacgctgtaa
    52621 gatggatgta atttttctag ttttttctaa taaattcggc atttccttca tcctttgtat
    52681 gttttgcgtt acaattatat caaagcattt tagcttttgc aaatcgatat cagtacattt
    52741 taagaacttc tattgttaat ttaatgttta tctaataaaa attcattcta gcgcttgtat
    52801 tggcttaaaa agcgagccat tttttcttgt agttcagtca attcatcaac acgtggtaag
    52861 taaacaattc taaaatggtc agcggtattc atattaaatc ctctaccgtg tgttagtaat
    52921 actttttctt gttttaagaa attaagcaca aactcttcat cattatcaat acggtacata
    52981 tctgtatcaa ttttagggaa aagatacagt ccagcatttg gttttacagc agataaacca
    53041 ggtatttcat taattgcttt atggatataa ttacgttgct cgtagatacg acctcctggc
    53101 aacaacatac tatcaataga ttgctgtccg cctaacgatg tttgaataac ctgttgagca
    53161 agaacatttg aacacagcct catgttagct aacatattca aaccttcaat ataaccttta
    53221 acatgttgtc gcggaccaga caataccatc caaccaacgc ggaaaccaca gatgcgatgt
    53281 gatttagata agccagataa agtaactgta aaaatatcct ctgcaataga agcaattggg
    53341 atatgctcca ttccatccat aactaaacgg tcataaacct cgtctgaaaa aataatcaag
    53401 tcattttgtc tagcgatatc cacaatctct tgcagaattt cacgcggata gactgctcca
    53461 gttggattat ttggattaat gaggacaatt gcttttgttt tggatgtgat ttttgatttg
    53521 atatcatcaa tatcaggata ccaattagct tcctcatcac aaatataatg cacagcattt
    53581 ccaccagcaa ggctaacaca agctgtccat aacgggtaat ctggcattgg aactagaacc
    53641 tcgtcatcat tatcaagcaa agcttgcatt gacatagaga ttccctcact aacaccatta
    53701 acaatgtata tatcatccat gtcaacatgg atgttttgta actggtaata ctgcataaca
    53761 gccttacgag ctgaaaaaat acctttcgaa tcagagtaac cttctgattc acgcgcattc
    53821 gtaattaaat cacgaatgac ttcatctggc gcttcgaagc caaaggcagc tggatttcca
    53881 gtatttaacc gcaatatctt ttcaccattt gccctcatgc ggtccgcctc ttctaaaaca
    53941 gggcctctaa tatcataagc tacatgttcc agcttcattg atttatcaaa tatcttcata
    54001 aaaatacctc gtatcttcca ttataaaaca aatattctca aaattgaaac ttttactgcg
    54061 gtttatcaag cgattaagtt aatattaagc tatttcagaa aactaacata aaacgctttc
    54121 atttttctag aaaaaacgtt ataatatagc tataaaatga ataaccaaca tatctaactt
    54181 actcatcact cactccttct gataagatgg ctagttgagt gcaagaggtt atctcttttg
    54241 gttatattta gaggaggaca ggcttatgtc acaaaaatat gaacgcatcc ttgtcgctat
    54301 tgatggatca acagaatctg aacttgcttt tgaaaaggca gttaacgttg ctcttcgcaa
    54361 tgattctgaa ttaatcttga cacatgttat agatacacgt gctttacaaa gctttgcgac
    54421 atttgatact tatatctatg aaaaattaga aaaagaagca aaagatgtcc ttgaggaata
    54481 tgaaaaacaa gctcgtgaaa aaggagcaga taaagttaga caagtcattg aatttggtaa
    54541 tcccaaaaca ttacttgcac atgatattcc agaaaaagaa aaagtagact tgattatggt
    54601 tggtgcaaca ggtcttaata cctttgaacg tttccttatc ggatcttcct cagaatacat
    54661 ccttcgtcac gctaaagtag atttactcat tgtacgtgac cctaataaaa caatgtaaat
    54721 ttaaatacgc tcactttcta aaatatcctg agttatatta actcaggata tttttatttt
    54781 cgatttaagg atttctgaat ttggctatct aattcacgtc gaattgatgg ctcaggagcg
    54841 aaatgttctt cccagtcgtc tggttttaaa attttatgag taacaggatc aaaatgagct
    54901 ttgccatctg gaaagatttt ccccatattg gcctcatgta ctgtatcaaa aagaggtttt
    54961 gggtcgactc ccataagaac aaaggagcca taagtcagat aaagtaaatc tgttaaggca
    55021 tccacttctc ccactaatgg tgtttctgga tggtctttag aaataacctt atctcttgct
    55081 ttatcaatag ctaagtgtaa attacggata gattggtcaa atactttttg gttaccttga
    55141 ctagcagcat acaagaactc aacgatttct tctactttaa agtctgacct gaaaccagct
    55201 tccttactgt catataagcg tggcgtttca atagtttcac catccattaa tagatggaag
    55261 gattttactt tattgaaatt ctcgtcacga ctactgaatg ttttttcaat ctcaaattgg
    55321 ataagtccat aatgggctaa tgccttagag ataccatcat cattattgtc agcagttgta
    55381 taacgtgcat tttctctcac ttgctgacta gcgttcccca tggcaacacc aatgccaact
    55441 tgagataaca tctcaatatc attatcagaa tctccaaatg ctatcacttc ggatagatca
    55501 aaaccatagc gttgaccaac acgttcaatt cccttaactt ttgactgccc ttcagaaatg
    55561 acatccattg agtaaggact gcttcttgtt aatttaacac gaggaaattg tttttgaatt
    55621 cgctctgatt cgccctctgt tgcaactaaa acgacctgat aaataggttc tcgtaagata
    55681 accatgaggc tatcaatatt ttgaggttta atccgacgaa taaaatgctt aaagcttcgt
    55741 tcaatcgctt ttgcccattt tctcggaaca aggctagata caatctgccc caaacggcta
    55801 gttcctagac caataatacc tgaaccgagt aatcctgagg ctgttcctaa tgaaatttct
    55861 cttcttcttg ctccagcata acgaatcaga cgatagacag ttgtcttaga taattgattg
    55921 gtataaataa tttcacttct actatagata tattgaccat tataggttac agcaaaatct
    55981 aaaccaaggt tttctaaaaa aggttgtaca aaactaggac ctcgccccgt tgctaaaccg
    56041 actagtattc cttggtcttt caaatttcga attgctttaa ttgttgactt ttgtacattc
    56101 ttacgatcat taagaagtgt accatcaata tcaaaaaata ctgccttaat tgccactggg
    56161 tcaacatccc ctctttttta tgataaaata aattatatca caacttgaaa gaagtataaa
    56221 atcaatgaaa aaaatccttg ttctacacac aggtggaacg atttccatga atgctaacga
    56281 gaagggacag gtcatgtcca gcgccgataa cccaatgaaa tacgttgatt tatcattaga
    56341 tgaccttgat ttgactgttg tagattttct aaatcttccc agtcctcaaa tcacccctca
    56401 tcatatgctt gatatctatc actatctgaa gcaacatgcc agtaattttg atggtgttgt
    56461 cattacgcat gggactgaca ctttagaaga aacagcatat tttttagaca caatgatact
    56521 ccctaaaatc ccaatcatca ttaccggtgc tatgagaagc acaaatgaac ttggtagcga
    56581 tggcgtttat aattacctat cagccctccg tgtcgctaat agtactaaag ctgccgacaa
    56641 gggggtgcta gttgtcatga atgatgagat acatgctgct aaatatgtca caaaaacaca
    56701 tacaacaaac gtttcgacat tccaaacccc tacacatggc ccccttggga ttattatgaa
    56761 acaagacctg cttttcttta aagcaactga agagagagtt cgttttgatt tagataaaat
    56821 tacaggaact gttcctatcg tgaaagcata tgctggcatg ggtgatagtg gtatcattag
    56881 ctttttaaat tctcaaaata ttagcggatt agttattgaa gcactcggtg cagggaatat
    56941 gcccccaaaa gctgcacaag aaatcgaaga attaattgag caaggtgtcc ctgttgttct
    57001 agtgtctcgc tgttttaatg gtattgctga gccagtttac ggatatgaag gtggcggagc
    57061 aaaattacaa gaatctggtg tcatgtttgt taaagaacta aacgctccca aagccagact
    57121 caaattactc attgcactca atgcaggtct cacagggcaa aatctaaaag actatattga
    57181 aggttgataa aaaactctag aaatatttct agagtttttt attaattttc agtttgtaat
    57241 actctctcaa cactgctaat aggcatttct tgattcgtta ttaattgaaa tgcttctgct
    57301 ccttgataaa tcagcatccc tagaccatta taagcatgtt tcactccatt ttgtctaaca
    57361 aatcgtaaca atgctgtttc tttaggcttg taaaccaagt cgacaactac taaattcggt
    57421 gttaaaattt ctggatcgtt aattaaacta taattatcta atggcctcat tcctaaacta
    57481 gtagcatcaa tataaaaatg actagttcta atagcgtctt taaatgctgt cttattttct
    57541 agataatcaa cgactacctt tatttgaaac tgttttttaa ttttatctga taagtcaatg
    57601 accttatcgt aatttgagct gttacgatta aataatctaa tttccgcaac tccctccata
    57661 gctgcttgaa ctgcaactgc tttacctgaa ccaccaatac cagctattgt aattatttta
    57721 tttttagcac tgaaaccttg agctgctaaa gctttaaaac aaccaatgcc atctgtcata
    57781 tggcctacta aacgtccggt tccaccttga ttaacgatag tatttacagc acccactaat
    57841 ttagcttgag gagataaatc atctagcaaa gggataacac tctgtttaaa tggcattgaa
    57901 acattaacac cacgaatacc caatgccctg acacctcgaa cagcttctgt taatttaccc
    57961 tcttctactt caaatgtcag ataggcataa ttcatgtttt tttcttgaaa agaggtattc
    58021 cacattaacg gggatagaga gtggcgtgca ggattggcaa ttagaccagt tagtaaagtt
    58081 tccccattta atgaattcat tgtataacct tctttcttaa caatttattt cagttcattt
    58141 taccaaaaac tgactcaatt aaaaagagag tatgtaattt atactctctc ccttagttta
    58201 aggcaatata ttacctgcac tacggtaaat atcataccat tctttacggg ttaaagcgat
    58261 atcattagcc ttatcaatcg cctttaaacg agatggattc attgaaccaa caatcgcctg
    58321 cattttggca ggatgacgta aaatccatgc aatcacaata gcttcgctgc taacattgta
    58381 gttttttgcc agtttagcta tggtttcatt taattcctta taatccggat gattaacaaa
    58441 taaaccacga gataaatcaa tttgaaatgg agaccaagct tgaatggtaa ctcttttaag
    58501 acgacaataa tcaacaatgc catcatcatg ttcaattgaa gctttattta acatgttaac
    58561 atttaaccct gcatcaaaca ttggtgtatg tgctggcgat aactgaagtt gattcactgc
    58621 taagggctcg tcaaggtagg attgtaacaa ctccatttga aaccggtttt gattgctaac
    58681 accaaagtgt tttactttac cttctgccct cagtttggta aatgcttctg ctacttcttc
    58741 aggctcaact aatacatctg gacgatgaag aatgagaaaa tctaaatatt ctgtccctaa
    58801 tctttctaaa ataccatcta ctgatgataa aatatactct ttagaaaaat caaagtaacc
    58861 ttctcgtatc ccacatttag attgaataat cattttgtca cgattgacat ttaaatgttt
    58921 gattgcatct cgaaagcgta attcagatag ccctccccca taaatatcag catgatcaaa
    58981 aaaattaata cccaaatcta atgctgttcc aactacttct tcagcttgtt ttcctttcaa
    59041 gtcactcatt ctcatacaac ctaaagcaat tcgagttgct tgaatgcctg tttgtccaat
    59101 gttttgtaac atatgcaccc cattctaatc tgtttgtatt aacagaaaga ttaatcaatt
    59161 cattataaca aaatttctat gaaaaggctt actattacaa atgcaaaaag ctgagtaaat
    59221 ttaactcaac tttttttctg atactcatta ttgactaaat aagtcggtaa gaaaaatcta
    59281 ttctgaaaag tactattata aattacagta atcattattg tcataacata cctccacatc
    59341 tgtgatagga atatcatatg acagcaagct taaactgagc ttagcacagc cttaatagaa
    59401 acttaatcaa aatctgaatg ttggcgtaaa ttatctagaa taagtgccca ttctgtattt
    59461 tctttccaat tattatcttt gacaatatca ctagcaacac gtctggcttc ttctaaaata
    59521 ggataatcct caacgatatc agctacttga aattctggaa ttccagactg acgagtacca
    59581 aagatttcac cagaaccacg catttttaaa tccgactcag ctaaaacgaa accatctgtc
    59641 gtttctgtca tgattgtcat tcgttttttc ccagaatcag ttttgggatt agccactaaa
    59701 acagcgtatg attgtttata tccacgacca acacgcccac gaagttgatg tagctggctt
    59761 aatccaaaac gatcggcatc cataataatc atgatcgttg catttgggac atttacccct
    59821 acttcaataa ctgttgtaga tactaaaata tgactttttt tatccttgaa atcttgcatt
    59881 atagcatctt tttcatcatt tttcatacgt ccatgtacaa gagcaacctt agcaattcct
    59941 tcaaaataag tagataattc agcatgcaat gctactgcat tctttaaatc taaagcttct
    60001 gactcttcaa tcaacggtga aatgacatac acttgagcat ctttttgcaa ttcacctttt
    60061 acccattcca acacagtacc tagctgctcg tgtttcaccc agcgtgtaat aataggttta
    60121 cgaccggcag gtaattcatc aataatagaa acatccattt ctccaaaagc tgtgattgct
    60181 agagttcggg gaattggggt ggctgtcatc attaaaacat caggattttc tcccttttca
    60241 cggaaaattc tacgctgttt aacaccaaaa cgatgttgct cgtctgtaat tacaagcccc
    60301 agtttatggt actgtaccga gtcctggata agagcatgag ttcctacaat catatcaacc
    60361 gagccatttg caatagctgc taaaaccgta cgcttaacag ctgctttcat acccgaagtt
    60421 aatatagcga ttgataaatc tggaaataac tcttgcagac taatgtagtg ttgttcagct
    60481 aggatttccg ttggaaccat caaagccgat tgaaaacctg ctgtataagc tgcatacatt
    60541 gatagaccag caatgactgt ctttcctgat cctacatctc cttgcaataa acgattcata
    60601 tgagctcccg atgacatatc agataatatt tcatctaaag agcgcttttg agcattcgtt
    60661 agaataaaag gtaaagagga aatttttgtc tccatagcat gtttactata aagaataggc
    60721 aaaccatttg tttcagattt attttcggat ttcaaaactt gaaggttcat ttgaaagtaa
    60781 aataattctt caaatttaat ccgacggagc gcttgcttat actctgtgat atcttttggg
    60841 aaatgcatag ctaaacaagc ctgactacga cccatcaatc ggtatttttc caataaagta
    60901 gctggtaaat tttccttcaa ttccaaatgc gcactgattt caaaagctga cttaatagct
    60961 ttaatcaaag tagactgtga aattccctga gctacgcgat aaacaggttg catgtcatct
    61021 tcaacttgag ctaaaacctt catcccagta atagctgatt tagttgcatc ccatttacca
    61081 aagacagcta cctcttgacc aagttctatt ttatcagcta aatagggttg attaaaaaaa
    61141 ctaacattta agacagcttc cccttgacgc aacttaaaac ttaaacggtt acgtttaaaa
    61201 ccataatatt gtacattagc tggagtaacg actaggcctg taatgactgc tttttcacca
    61261 tccactaaat caaaaacaga ttttgattta aaatcttcat agcgaaacgg ataataaaga
    61321 agtaaatctt ctactgtata aatatctaat ttctgaaatt tttctgctga ttttggtcca
    61381 aatcccttaa gattagaaat tggtgattgt aatagcataa gattctcctt ctaatatctt
    61441 ttattatact aaaagatcac caaaaaggcg cctcaagagc gcctttatta tgtctaatta
    61501 tttatggcca ataacataag ttaattgtgc agcttgtgct gaactaaaag tacggtaaga
    61561 gacaatacca cgtcccattg cattagattc tgaaataaga actgaaccat cttttttaac
    61621 ttcttcaaca atagctacgt gaccgtaagt gccatcagca ccagcttgtc ctggtgaaaa
    61681 tgatacagca tagcctactt ttggtgaatg tgttgtttca aaaccagcct tatgttgcca
    61741 atctccacca ttacccataa atggatcaaa tgtataacct aactctttag cgcggttaaa
    61801 gacataccat gtacattcac cccatggata agttgatgca atatagctag ctgtgttaac
    61861 tgctgttgat aagctaaaac cacttggtaa atctgctaaa cttgctgtcg ataaagcttt
    61921 caaggcagca ggaatttctg gtgtccctcc atgttgatca atccagcggt ctaaatcttc
    61981 cataatctgg gcacgacgtt taccagttcc agagttatct gtataataaa caccaccctc
    62041 agtaactgta ttaagttgtc cagaagataa tttctgtgct ttcaaatctt ggatttcaaa
    62101 gctagagttg ttgtttttaa taattgtatt ttttgtcaac aacataattg cttcttcatc
    62161 attataagca ctagcatggc tagagtcatg atcaaaggca ccataaccaa acatattagc
    62221 acctggcatc ttagccactc cttgagttcc caatgaactt tcaaggacag caatagctac
    62281 aatagcacgg acatctaaac cactatcttt ttcccattgt aataacttag caccattgat
    62341 acgattagaa tcatattgaa tccctaaaga ttttaagtac ccatctaatt gttcagcttt
    62401 gataccgtaa cgatgtgaca ataaatagtg agagtaggca tcctgaccag accagtgttc
    62461 tacgtagaat ggcgttgctg tcactggtga aatcggtgtt gataataagt tatcatcaaa
    62521 ggataaagca ggaacagaag cattctgatt tgtctgaaca ctttctatct gagacggagc
    62581 gggagttatt ttaggctcta ctaatggtaa ggtaggctca ggttttaatt cagtctttgt
    62641 tgattgacct ggcttactgt ctgcttgact actgtttgta gtattagtgc taggagtaga
    62701 tgctgttgaa gcagaatcag atgttttagc atcttcgttg gaagaagatt gatgtgatga
    62761 acttgatgat gattcattgc ttgatgatgt ctctgtcgta ccaatctctg ttttttctgt
    62821 tgagatactt ccttcggctg aaggtgaagc agtttctgaa tctgtaggtt gtgttttttc
    62881 ctgtttctct gcttcagaag aactttctgt tgatgctgtt gccattgtca ggcttgtttc
    62941 tggattaata gtttctgcta aggtcactaa aggtgatgat agcgataaga ctaaagtgga
    63001 taacaacaaa gttgccatta ctttttgttt tgacatatac taatgatttc cttctataaa
    63061 aattgtcact gattagttta acatattttt aaatttttta aaacagttaa ctactgtgtc
    63121 atttgttttt aataatatct cggaatacga tcgcttaaaa gacaaagtac ttcataatta
    63181 atcgtctgtc ttttttgagc gacgtccgta gttgtaatgt tacaactacc ttgttgacca
    63241 atcaaggtta ctttagttcc gatagtatat ttttgaggta aacggatagt catttgatcc
    63301 atcgagacac gcccaataat ttcacaaagt tctccattta ctataactga aaatccttgc
    63361 atatctcgtg tccatccatc agcgtagcca atcggtactg ttccaacaaa ttcgtctcca
    63421 gtaacttgat aggttgcgcc atagccaact tgagaaccat cgtgtagttg tttaacatgt
    63481 actagctcag actctaaact taaagcaggg tttatagggt aaggtaagtc taaatcagtt
    63541 ccacttggat ttaagccgta cataacaaca cccaatctaa cggcattaaa aattgtctca
    63601 ctatgccaaa ggcttgtagc agaattacta gcatgaacaa ggtccggaca attatctaat
    63661 cctgaaataa agtctttaaa aaaagtaagt tgttgattga atttacagtt atcggcttca
    63721 tcagctgttg caaaatgggt gaaaatcccc ttgacagagg caccactttc ccccaattca
    63781 gaaattagct tatttccttc atctaactgg cgaacaccta tacgtcccat tccactatca
    63841 actttgatat gtacttctag accagataaa tctactgctg atgtctgaca taaacgtaac
    63901 cactccaagc tagcaactgt tagagtaata ttttcatttt tggctagtat aacctgctca
    63961 ggcataacaa cacctaatac caatatcatt tttacaattc ctgcttgacg tagctcaata
    64021 gcctcatcta aattggatac gcaaaaacca tctacaatag actcaatgta ttttgaaact
    64081 tcaacagcac catgcccata agcattagcc ttaactacag caaatgtttt tttatcactt
    64141 ggaatgtgct cttgtacaga tttaacgtta tttgcaatcg cctctaaatc tatcaaggct
    64201 ctggttggtc tatgataact tgaaatcatt tttcttcctc caaaataaca ctagcttgtg
    64261 cataattacc actatgagat attgaaacaa aagattttcc attaaacggt tcttttgtaa
    64321 tcaaaggcgc tcctttatca tccgataaaa tttcgatatc atgaaaatta actttcccaa
    64381 ttcctgttcc aagtgctttg ctataagcct cttttgctgc ccatcgccct gttaaaaaag
    64441 acatctgacg cttgggattg gaaattcctt taaaaagaag caattcttgt tcggtcaaaa
    64501 cgcgttctgc aaaacgttga ttacgctcat atgctttagt aatcgcctct atctcttgta
    64561 aatcaattcc gtgtccaaca atcatgtgta actcctataa gttcgatact tacaaactat
    64621 tgtaaatatc cctaattagc atttctgttt tatcccaacc tagacatgga tctgtgatag
    64681 acttaccaaa aacatctggt ttatcttgtc ttccatcttc taaataagat tctatcaaaa
    64741 aaccacgaac atagtttcta attttagtat gccactgtcg gtctgcaagt actgatttca
    64801 caatgcgaat ttgttctaaa aattgcttac ctgaattgtc atgatttgta tcaacaacaa
    64861 taaagggatt ttgcaaatca gtctcttcat aatgagtaat agtttctaaa aggttttcat
    64921 aatgataatt agggatactt cgatagtcgg cattatgata tcctctcaaa ataacatgtg
    64981 acagcaaatt accatctgtt ctaacttgct tattttgata gaataattcc tgttgatttt
    65041 gtgctgcata gaccgcatta aacatgactc ttaaattacc agacgttgga tttttcatac
    65101 ctactggtgc atctatacct gaagatataa aacggtgtcc ctgatcttcc actgaacgcg
    65161 caccaatagc atagtaagaa actaaatcat ccatcactga taacatctct ggataaagta
    65221 attcatcagc tgtcaggagt ccagtctctc gaatgatgtc atagtgcatc ttacgtgctt
    65281 gaaaaaaaac tcctagtttg tcagaatggt gaactaagcc tttataacca tcaccattcg
    65341 ttcttggctt agcggtataa actctcataa ccaaaaacat tttatcttta aaggcagctt
    65401 ccaatttagc taaacgcttt gcataagtta gcaccgcctt tggattatcc gcagaacaag
    65461 gacctactat tactagaaac cgctgatcct caccttttat gatatttttt aactcttctt
    65521 ctttggctaa ttttttttgg ctaacttcta gatctaagct agttttctgc ttcaatattt
    65581 caatattaat tttatcactt aactgatgaa atcccatgtt ttttcctaat acaacctaag
    65641 tctattaata agctctgcta tacaatatag tcgtcatact attagacaag taatgataaa
    65701 acagtcttct cagactgttt tatttttatt ttaaaatcat tgttattgta aacatctcag
    65761 accatgacaa tttttaaact tcatgccaga gccacaagga catttatcat tacgtttaat
    65821 cccttggtat tctggtgatt cattattcat aggtacatgt tgtgccgaga tattttgctc
    65881 tgctgttgtc gtagcatgtt gagaagcacg ttcacgctct tgttcatgaa tttgagcttt
    65941 catcaatgta cgtgtcacat caaactcaat cgaaccaatc atatcttgga acatacggaa
    66001 accttctgat tgatactcaa cgattgggtt attttgagca taaccacgaa gtccaacgga
    66061 gttacgtaat tgatctaagg catcaatatg ctctgtccat tgattatcca ctaccatcaa
    66121 gattaagact ttttggaatt cgataatagc ttctggatct tttaatttag caatttgttg
    66181 ctcataaaca gctaaggcac gttcataaag aagttcttta atttcagcct cttttagacc
    66241 acgaagttct gaaactgtga tagcttcttc atctaataac gccgatcttg caaaattaac
    66301 aatagcttct gctgcagtat ttttatcgct tctgctatgt gcatcaactg ctcttttaat
    66361 cgtacgttta atcatccctt tgagctctgg tcctaaatca cgctcagcag taatcacttc
    66421 tctacggtta gcataaataa tctcacgttg ttcacgcata acatcatcgt attgcaagac
    66481 ttgtttacgt gtgtcatagt tattaccttc aacgcggcgt tgagctgatt caacttgtcg
    66541 tgtcaacatt tttgatttaa taactgtatc atcttcagct aaattcattc gctcaagaac
    66601 cactttaata cgatctgtac caaagcgacg catcaaatca tcttctaacg aaagatagaa
    66661 ttgtgattca cctggatctc cttgacgacc cgaacgacca cgaagctggt tatcaatacg
    66721 acggctttca tgacgctctg taccaataac acataaacca ccaagttcac gaaccccttc
    66781 accaagcttg atatcagtac cacgaccagc catgttggtt gcgattgtaa cagcaccacg
    66841 ttgcccagca ttcataatga tttgagcttc tttaaagtga tttttagcat tcaaaacttc
    66901 atgtggtact ccagcagcaa ctaattttct agaaattaag tctgacgttt caacagctac
    66961 tgtaccaacc agtacaggtt gtccttgttc ataacgttct ttgacatctg ctaccaccgc
    67021 tctaaattta gagtcaagag ttggataaag tagatctgaa tgatctatac gttgaacagg
    67081 acgatttgtt gggataggaa taacgcgcat attataaatt tcacgaaact cttcttcctc
    67141 agtcttacca gtaccagtca ttcctgctaa tttatggtac atacggaaca tattttggta
    67201 agtaattgaa gcgctcgttt ttgattcttc ttggattggc actgactctt ttgcttcaat
    67261 agcttggtgt aagccatctg agaaacgacg tccttccatc gtacgaccgg taaattggtc
    67321 cacaattaaa atctcttgtt cttcactaac cacataatca atatttaaca acataatata
    67381 gttagctcga agagcgttat caatgtagtg agttaaggca acattttcga gatcatacaa
    67441 gttatttaga tggaaatagt tttcagcctt gtcaatacct gtatctgata aaccaattgt
    67501 ttttgttgga acatcaatga tataatcatc gctattaagc gtttttacaa acatgtccgc
    67561 acgtgtatac agttggttca tctcagagct tacaggacct gaaacaatca atggcgtcct
    67621 agcttcatcg attaatacag agtcaacctc atcaaccaaa gcatagttga gtgggcgttg
    67681 caccatatct tcttggcgaa caaccatatt gtctcgaagg tagtcaaatc cgacttctgc
    67741 gttagttgag tatgtgatat cacaattgta agcttcgcgt ttttcaaatg gtgacttagc
    67801 tgctaagtta atccctacag ataatccaag ccaactataa acctcaccca tttcggtcgc
    67861 atcacgtgtt gataaatact cattaacagt aataacatga acacctaatc ctgagatagc
    67921 atttagataa acaggcattg tcgctgttaa agtttttccc tcaccagtac gcatttctgg
    67981 aatatcacca tggtgaagaa cgataccacc cataatttgg acgtggtatg ggtataaacc
    68041 taatacacgt ttagaagctt cacgaactac cgcaaacgct tccggtaaca attgatctaa
    68101 agtttcacca ttttgataac gttctttaaa ttctggagtt tttgcttgta aagcttcatc
    68161 tgataacgca gccatatgat ctgcgtaaga gtcaactttc ttagctattt tatctaattt
    68221 ttttaattca cccttgtcat tttcaataac agtgcgtaga atatttgcca ttctaattcc
    68281 tttcgatact cttatcacca cattttacca taaataatgt tgtctttcaa taaatggtcg
    68341 ttttaatcga gattaaaaag agtggtatcc ccactctcat tattttttaa catgacttgc
    68401 tataatttct aactgtccat caatatccca tttgacaaca tcatttggca agataaagtg
    68461 gtcacctttt ttaagtgtat aaactttgtt atctactgta atgtgacccg ctccgtctaa
    68521 gacactaacc aaaaggtagg gtgcaaattg tttaaagttg gtaacaccag aaatttccca
    68581 tttataaact gtaaagaaat catttgaaac taataaagtt gaagatagat gttttaactt
    68641 catattggca ggaacagtat tggcaggttt accaatcgtg agaacatcta ttgattgttc
    68701 aatgtgtaaa tcacgtaatt ttcctgaagc atctggacga tcaaaatcat aaacacgata
    68761 agtagtatca ctagattgct gtgtttctaa aattaatatc cctttaccta tagcatgcat
    68821 tgtcccactt ggaacgtagt aaaaatcccc tgatttaaca ggaattcggg ttaaaagatg
    68881 ttcccaatct ccagattcaa tcatttggcg aagttcttct ttagttttag cattatgacc
    68941 atagataatt tcagatcctt catcagcaga aataatatac caacattctg ttttaccaag
    69001 ttcaccttca tgtttaagag cataagcatc atccggatga acctgtacac taagccagtc
    69061 attagcatct aagattttag ttagtaaagg aaagacagta tcatcagggt tgccaaataa
    69121 gcttttttca ccttgataca gcttatctaa aaaacaacct ttaaagcgac cattcttaac
    69181 tcttgagaca ccgttaggat gagctgaaat agcccaatat tctcctgttg tttcacttgg
    69241 aatatcatag ccaaattcat cccttaactt tgttccaccc cagattttat cgtgcatgct
    69301 tgcttctaaa aataatggtt ccgacataag aatcccctat cttttgtaaa attcaagcct
    69361 attatagcat tttatccatc atatcgctta ctttttttaa tcttttattt gctaactaaa
    69421 ctaagtagtg gcttaagggt tttattaacc ttttgatatt ttctttgcta aagcaaaatt
    69481 tcctaacgtt gcagaaccat tttcttctat agctggtgtc acaatataat cagacaaatc
    69541 gggaactggt aaatacccat ttaataaagt tgcaaatgtc tggcgaacac gtcttaacat
    69601 gtgctcttgt gccatgacgc ctccaccaaa tacaatgact tgtggacgat ataatacagt
    69661 tgcttgtatt gcagcctgag caatataata ggcttgaaca tcccaaacca ttgaattttc
    69721 ttcaatcaat tcaccacgaa taccagtcct agcttctaga gttggaccag atgccaaacc
    69781 ttctaaacag ctattatgaa acggacagat acctttaaaa tcatttgctt ggtcctgcgg
    69841 atgcatcgcc atataagtat gacctgcttc agtatgacca gttcctccta taaattcacc
    69901 cttttgtata gctcctgcac caattcccgt accaatggtg taataaacaa gtgaatcaat
    69961 attgttacga gctataactt caccataggc agagctatta acatctgttg tgaaataaaa
    70021 tggaacattg aaatctttag aaattaagcc gagtaaatca acattagccc aatgcaactt
    70081 tggggtcgtt gtgatataac cgtatgtctt tgatttttta tcaatatcaa tgggaccaaa
    70141 agaaccaata gcaactgcct ctaacttctt ttcaaatctt ttaaagaaat caacagtctt
    70201 tttaatcgtt tcttgaggcg tcgttgttgg aaattgcatt ttttctacaa cttttaattc
    70261 ctcatcacca actgcacaaa caaactttgt cccacctgct tcaatactac catataactt
    70321 tgtcatcttt tttcctatct ataatctcta tctcaaagac aaaggttggg ataattatcc
    70381 caacctttaa tcctcactta acatcaataa tcatgtcacc tgtagtaaca acaccctctt
    70441 ttaccaatgg tacaacatca gcaaaatcag ctgtattagt cacaataatc atagctgtat
    70501 tatccagacc tgcttctgca atcttggctg aatcaaaggt gccaagaata tcaccttttt
    70561 tcactttttg gtctgccttg actttagaag taaaccctgt gccattcatg gtgacagtat
    70621 caatacctat atgaatcaaa atttctgcac ctttatctga tttgataccg taagcatgac
    70681 ctgtttcaaa agcaatttgg acaaaaccat caacaggtga gtaaattgta ttacctcttg
    70741 gtttaatagc aaggccatct cccataatac ctgatgaaaa aactgggtca cttactgtat
    70801 caagagcaat aacctgtcca tcaagagggc taacaatcgt ttctgccctt aattggctag
    70861 tttgagtaat tgttggatcg tcatctgttt cttcaaccaa aggactaact tcagctactg
    70921 ctttttcatc tttatcttca tatccaaaca tataagttaa tacaaaagcc aaagacgtag
    70981 ttccaattac cataataagg tacttaacaa tttgaccatt taaataaagt aatgttcctg
    71041 ggataatggt aataccaaaa ccagttcctg ctaatttcaa tattgaagct aaccagccac
    71101 ctgctgctcc tgcaattaat cccataataa atggcttacc aaaacgtaag tttactccaa
    71161 atatagccgg ttctgtaata ccaagtccag ctgaaagcgc tgccggaaaa gcaagggctt
    71221 ttaatttttt attttttgtt ttaacaccaa ctgccaaagt ggcacctgct tgtgctgtca
    71281 tagcagctgt aataatagca ttaaatggat ctttcccatc agcagcaatt aactgagctt
    71341 caagtaaatt aaagatatga tgaacacctg taacaacgat tatctgatga acaccaccta
    71401 aaataagacc tgacaggcca agaggtaaat taaggacaaa ttttgtccct gctaaaacat
    71461 agttttcaac tgaatggaaa ataggaccaa tgacaaaaag tgctaaaatt gacataactg
    71521 taaaagttag gaacggcact aatagtaaat caagaacatc tgggattttc ttatgcaacc
    71581 atttttcaag tttagcacca agtaaaccta caaagaatgc cggcaatact gaattttgat
    71641 aaccaactac tggaataaaa ccgaaaaatt ttatgggatg ggcatcacct gaggccactg
    71701 cccaagcgtt tggaagggca gagttgacca tcatcaatcc taatacgaga ccgataacag
    71761 gatttccacc aaaaactcta aaagctgacc aagaaatcaa ggcaggaaaa aaagcaaagg
    71821 ccgtgtcagt caaaacgaca gtataggtat aaaaattaga acttgaaaat gcttttgaag
    71881 tcgtcccaaa taaagctaaa actgtatcat tattaatcgc accgcgaatc cccataaata
    71941 aaccagtagc aacaatggct ggcaacaatg gaacaaaaac atcaccgaaa gtacgaatag
    72001 ctcgttggaa agcattccct tgtttagctg cttctgcctt ttgatcagag gttgacgaag
    72061 ttggcaaacc ttgagccact acttcatcat aaatcttatt aacagtacca gttccaaaaa
    72121 taatttgata ttgacctgaa ttgaaaaatg ctccctgaac tttttcgata ttttcaacag
    72181 tatttttatc aatgactgtc tcatctttta ccataacgcg aagacgagtg gcacagtgtg
    72241 ctacgctacg cacattacta gcaccaccaa tcgcattaat gacttgttta gcaatttctg
    72301 tatttgaagc catctataaa tctcctttga tattttgaaa gcgtttcatt ttatttactc
    72361 taatattcta gcacgtctca taaatatatg tcaagcgttt gacataaaat aatatttttc
    72421 gatataaagt gttgatttca aaagagaaaa cgtttactat atagttatta gatattaaga
    72481 cagtcaagga gtatcaaatg aatttaccca ctgaaatccg ctatcgtcct tatgatgaat
    72541 ggactgaaga agataaggaa aatattgtaa agaatgtctc aaaatcccct tggagagcaa
    72601 cttatcactt agaagctaaa acagggcttt taaatgaccc aaatggcttc tcttacttta
    72661 atggaaaatt ccatcttttt taccaaaatt ggccctttgg agctgcacat ggtttaaaac
    72721 aatgggtaca tacagaatca gatgatttag ttcatttcaa agaaacagga atcaagctta
    72781 aacctgacca tgtcaatgat agccatggtg catactccgg ttctgcatta gccattgacg
    72841 ataagctttt tcttttttat actggtaatg tccgtgacat gaaatggaac cgtgaccccc
    72901 gccaaattgg agcatggatg acaaatgatg gcaagattac aaaatttgat aaagtcctta
    72961 tttcacaacc aaatgatgtc actgagcatt ttcgtgaccc tcagattttc aattatgata
    73021 atcaattcta tgctgttatc ggggcgcaaa atagtaaaaa atgcggattc attaaattat
    73081 ataaagcact taataatgac atccatcatt gggagtttgt gggagattta gattttggtg
    73141 gtactggatc tgagtatatg attgaatgtc caaatattat ttttgtcaaa ggaaaacctg
    73201 tattacttta tagtcctcaa ggactagata aaaacgaatt ggactaccaa aacatttatc
    73261 caaataccta caaaatcgga caatatttcg atgctaactc atctaagata gttgaaccat
    73321 cccctatcta taatttagat tatggttttg aagcctatgc tactcaagga tttaacactt
    73381 ctgatggtcg tgcctttatc gtgagttgga taggcttgcc agatattgat tatcctagcg
    73441 atcaatttga ttatcaagga gctatgagtt tagttaaaga gttaagcata aaaaatggga
    73501 acctttatca atatcccgtt cctgctatga aaaatcttcg tcaacatcaa gcagaattta
    73561 aaacacaact tcaaactaac aacacctatg aattagaatt actggttcct agaaatgatc
    73621 tttcttcatt tgttctcttt gctaatccga aaggacaagg actatccatt actattgata
    73681 ctgtaaaggg gaaagttatc attgaccgct cacaagccgg acagcagtac gccacagaat
    73741 ttggtactag tcgccagtgt gacattccaa aagatgctac tagtatcaat atttttatcg
    73801 ataagtccat ctttgaaatc tttataaata aaggagaaaa agtatttacg ggacgagtct
    73861 ttccagatgc agaacaatca gggattcaac taaaagaagg gcacgttcat ggtaaatatt
    73921 ttgaattaaa atattaatat ggttgcaaaa ttaacagatg ttgctgcact tgccggtgtt
    73981 agcccaacga ctgtatcacg cgttattaac aaaaaaggct atttatctca aaaaacagtc
    74041 accaaagtca atgaagccat gagaaccctt ggttataaac ctaataattt agcacgtagc
    74101 ttacagggaa agtccgctaa attaattgga ctcattttcc ccaacatccg taacattttt
    74161 tacgctgaat taattgaaca ccttgaaatt gagttgttta agcatggtta taaaactatc
    74221 ttatgtaata gtgaaaagga tcctatcaaa gaaaaagaat acctcgaaat gctaggagca
    74281 aatcaagtag acggtatcat ttcaagtagt cacaacctag gaattgatga ttacgagaaa
    74341 gttgaggctc ccatagtagc attcgataga aatttagctc cacacatccc tatcgtctca
    74401 tctgataatt tctttggagg aaaaatggct gctcaaaccc ttaaaaaaca tggctgtcaa
    74461 aagatgatta tgattacagg gaatgataat tcagattctc ctactggact taggcggcta
    74521 ggtttttcct atgaatcaaa ggaatccaaa gttatcacag tcacaaatgg gctatctaac
    74581 atgagacgtg agatggaatt aaaatcaatt atctcgactc acaaaccaga tggtattttt
    74641 acatcagacg acttaactgc tttattagta atcaaactta tcagccaact agggttgtcc
    74701 attccagaag atatcaaagt gattggatat gatggtacaa gtttcattca agattacgtc
    74761 ccacatctaa ccactattaa acaacctatt cgagaaattg cgcaattgat ggttgagata
    74821 ctcctagcta agattgaggg gcaaaagaca aataaggatt atattttacc agtcagtctc
    74881 atccctggta gtagcgttta ggaaatcata tttatgaaat aatttttagt accgatagtt
    74941 atttttcaaa gcaaattaaa aatgtggaag aagattcttc cacattttta attttcattt
    75001 gttataaatt gacttaataa accattgaca aattttgctg acgtctcatc tgaatatttt
    75061 ttagcaatct cgataatctc atttagagct acacgatcag gcgtttcatc aaaatattta
    75121 atttcataga ggcctaagcg taagagagat ttatcaacca atgttagacg ttctaagctc
    75181 catcctgatt ttaagtgact agaaatcaaa gtatctaatt catctttgtg atctacaaca
    75241 ccattaacaa gattaagtaa aaagattgga acctctaaaa ctttgtcttc agatactgtt
    75301 ttatcataac cataagcaaa gtgagccgca tcaataaact ctccaccagt ttctaagcta
    75361 aatagtgttt gaaaagcacg ttcacgtaaa tcacgtcttg aatctttaaa tacactagtc
    75421 atctaagaaa tcctcatcaa acaatgactt caagtctggt tttggtgttt tctctggaac
    75481 aataccttca acatggatat tgaccgaaga aatttttact tctgccatgt catatacagc
    75541 tgttttgaca gcttgttgaa tagcaattga aactgctggt acatttacac catactgcaa
    75601 gtaaacataa atatctgcag ctactgtatc atcttcttca tttttaaggt agacacctct
    75661 acctagagat ttcttactaa ggctatccgt taccgctttg ttacgtaacg aatgaacacc
    75721 atcaacttta gttgcagcaa tacctgtaat tacctcaagt acacgtggtg aaatgacaat
    75781 ttctccaaga ttttcagttg tcatagctta tcctttcttt tagtgtttac taaggttttt
    75841 ccttaataga gtaaattcat catgaatcta cttaacactt ttttgagagt cattaggcgc
    75901 gtgaaacgta agttccttct gctgtattga ttactaactt ttgtccagct tcaataaaat
    75961 ctggaacgtt aacaacaagt cctgtttcca tagttgcagg ttttcctgaa ccagttacag
    76021 tagcaccttt gattgatggt tgagtttcag caactgtcaa ttcaacagtt gttggaattt
    76081 gaacaccaat aacttctgtt ccatagaatt gaatttttac ttctgagttt tcaagaatgt
    76141 agagtaattc attttcaatg ttaacagttg gaatttcata ttgatcgtaa gtttcattat
    76201 tcatgaagta agctgtatca tccatcttat aaaggtattg agcaggtaca gtttcaataa
    76261 tagcttgctc aaatttttct tcaggacggt aacttgtatc aaaagttgaa ccagtacgaa
    76321 catcacgcaa tttcatacgc atgattgtgt tacctttacc tggtttgtgg tggctagctt
    76381 caagaacacg gattaatttc ccatcagccg tttcaaaagt catgcctgct ttaagcttac
    76441 ttgcttcaat catttattaa tacctctttg taaaaattat ttatcaactc attttatcac
    76501 aaataacctg ctttctcaaa tagtttattg atttggtatg tgtctgattt cttgataaat
    76561 aaataaaatc accaagaggg cgtaaataag actgaagcac ttgacacttt cctcttactt
    76621 cagttttgat aacagtttta atattatacc gatatcttga ctaagattga aatactgttt
    76681 aggtgttttt agtgactttt tagtaaattt tgataaaggt agtaaactat agttgtctta
    76741 ttgaaacaga taaaataaat cacctacgga gctattcgta tgaaaatgta gttattctcg
    76801 atataagttt tagatacgaa cttaatttgc tctattgaag ctggtaaagg aaatcggggt
    76861 ggtgttagtg gacgtgcgct aagcattggt actgagcttg gtgataccaa tcttgcttac
    76921 tacatcaggt agctctctgt tttagtgcca tatgcaattg gaataagagc tgtcattggt
    76981 attgaaggag ccctagtcca ctgatcattg aattactaat ttaggaggtg gaatgatgaa
    77041 ttttaatatg tctcattata gaaagttaca actatgtcgt tttctaatcg tacagatacc
    77101 tgcgctggct ttattagcta ttcttttata cgggattttc ctaaagtaca gtctattttc
    77161 aaaagtattt tttcttatct tttaggggtt gaaatagtta tcaattttgg gaagtacata
    77221 ccagtttaca ccaaccacaa tttgtcgagt aaagaattgt aatttagtaa agcccttttc
    77281 gttttcttct gataagtgta tcatgacatc tatcaatagc atattcacac tagtagcata
    77341 ttgatttgtt tttgagtaga atttactttt tagattattt ctattctaca ctagtctttc
    77401 cctttggaag attctttccc tctgtatttt tccatttttc aatgagacga gaaactcttt
    77461 cttcctcctc atcatggtaa ccttcaaaaa caacctcatc aatatcttct tgattaaaat
    77521 agtaaacttg ttcaagattt aaaccctcag gaaaaattcc acctaggtaa tcaaaataaa
    77581 cttcttgtcc ttcttgctca acaattgcac ctctatttac aataactaat ttttgattgc
    77641 catcaataag atagacaaca ctccctactg gtaataacat acttcagttc tccccaatat
    77701 aataattttt cccatttaac gatggttgaa actcaatgaa ctttataaaa aataaactgt
    77761 ttgaaaaaat aaatccaaat aaaaataagt ttgaaaag
//
LOCUS       DATRMA010000002        75461 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.002, whole genome shotgun sequence.
ACCESSION   DATRMA010000002 DATRMA010000000
VERSION     DATRMA010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 75461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 75461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..75461
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.002"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            61..1611
                     /locus_tag="VBC28_000076"
     CDS             61..1611
                     /locus_tag="VBC28_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014619948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEO6814088.1"
                     /translation="MPYAIDLFCGAGGFSEGILQAGFDIIFSSDKSPMVEETYTNRHK
                     QLGLVEGVDTHFELADIKELTSDKIFQSINSLKYGNIFERGTIDVIFGGPPCQGFSRL
                     GKRDSKDPRNMLFHEYLRIIKDVMPRYVVMENVTGILDMQMLDFPSVLNDRIYGGQHL
                     VQSILRNELEELGYTLLDVQVLNAANFGVPQQRNRVVFLAYRNDVAPIQYPESDNDIP
                     NATVYDALGDLYGGEFSYETNYSLQSRLGRTPNTDGHPITNDNPLNMEISRHDDIVTQ
                     RFSLYQQGENRAKAVNRIQLEGLELKRDYPNLFIESLFQINGKANQLHIIKQLKKQDL
                     LQESFKSERWLQFTNRQLGLLSMNDTDNSAKQHILKSLALRLKTSFEEAELFWNEIKN
                     QLNSDITEDKFSKMLRDGDLTPEAIEAIFTKKSIRVRLDQNSVSPTMVTLPDDYIHPF
                     FNRVLTVREMARLQSFDDSFEFLGKRTTGGDKRAKETPQFTQVGNAVPPLLAKAIAEQ
                     VMIALNND"
     gene            1699..2061
                     /locus_tag="VBC28_000077"
     CDS             1699..2061
                     /locus_tag="VBC28_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6814089.1"
                     /translation="MKKILSLLLLGITAIFLISCSKQNSLYGKYYDIYDGKAKLILEF
                     NENGGRFYENETRAITNIDTKNKTFTFSVNGRDVVVTYDLKENGTLKYDTGSYFTSGN
                     ENIAYKKDSEAYKKALKK"
     gene            complement(2081..2932)
                     /locus_tag="VBC28_000078"
     CDS             complement(2081..2932)
                     /locus_tag="VBC28_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002280069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEO6814090.1"
                     /translation="MQRIYFNVWNRVKQTLYPFMKLNKVHPLYYTFQDYFNYEVDKKN
                     ILVIPHHIRIDIAGFNINDDVLTISYQDSDSITRQNFTKCHELGHILLHHSGRVFTDF
                     HDDSLQEREANFYSAFLLMPDIVLLTKIFYQQMAFQEVEAALQVSGQALKTRLTDFLN
                     FELDINRLSAKQIVEDYLTRKNKHIVGYFDQVKDYIISDYNEFEPSALDKFNYLINQS
                     NFISDIDLPELADTQFTSLIKQCYPKFKIWGLYDKGQSISYAWNPKQVTEVEAKRHAK
                     LLLITRK"
     gene            complement(2939..3301)
                     /locus_tag="VBC28_000079"
     CDS             complement(2939..3301)
                     /locus_tag="VBC28_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002280070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO6814091.1"
                     /translation="MFPERLKALRLEANLTQSDVAKEFGISQQAYAKWENAKANPTQS
                     VITKLANFYNVSIDYLMGKSDYKNSDEIDLSTFEVLYRKTSKNLSDEEKKILEEDLRE
                     FLLEREKAIRAMNKEKKD"
     gene            3982..4254
                     /locus_tag="VBC28_000080"
     CDS             3982..4254
                     /locus_tag="VBC28_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014619943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HEO6814092.1"
                     /translation="MATVPTQIRIDETVKAQATSLFNDLGMDMSSAVNIFLRQCLLRG
                     GLPFTVEVPNYSQKTLEALAEAKRISRDKTIASYDNLADLKKALDA"
     gene            4255..4533
                     /locus_tag="VBC28_000081"
     CDS             4255..4533
                     /locus_tag="VBC28_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafQ family
                     toxin"
                     /protein_id="HEO6814093.1"
                     /translation="MYQVKFTSTFKKSYKRIKKRGLEMTLLDEVIEKLRLGQTLEEKY
                     RDHELKGNFVGFRECHIKPDWLLIYLIEDDILTLTLADTGSHADLFKM"
     gene            4662..4898
                     /locus_tag="VBC28_000082"
     CDS             4662..4898
                     /locus_tag="VBC28_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003034655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6814094.1"
                     /translation="MNDYEQSFRRIYENFVFSLKIYPDKHYQKVLCYQVLEKMDKLFH
                     DYQKLGLPTVQLVQWKNHYKFKVQHDYIMKGGTT"
     gene            4895..5122
                     /locus_tag="VBC28_000083"
     CDS             4895..5122
                     /locus_tag="VBC28_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017827899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEO6814095.1"
                     /translation="MNYFEQRFQQIYEKFLFSLKIYHANPTHCETCYRDCLNEMDSLF
                     LRHDTHDQFAKQLLNCKKTFQFKVKKAYFGM"
     gene            5236..5550
                     /locus_tag="VBC28_000084"
     CDS             5236..5550
                     /locus_tag="VBC28_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6814096.1"
                     /translation="MEITKVFVDQNQVLGNEVFVSGMEEVRANDYNPVSYRIFEISSR
                     KEPSAFLIVEGFKMSDVPEGRQKVVFPRGIAIERRNFRFGGKRKHENVIVAEEMRTVK
                     SK"
     gene            5573..5983
                     /locus_tag="VBC28_000085"
     CDS             5573..5983
                     /locus_tag="VBC28_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003034661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF961 family protein"
                     /protein_id="HEO6814097.1"
                     /translation="MTVKFAADVIETFDTVKTLGTLNFLEAIPFMHWEDYVEEGTGEE
                     KRRETDKVDYVDVKLYSSAVNGDITVTVPPEAKVAQMTAEKNYNDEVTLVAPTARFWS
                     NAETINNRRITTSGVKLRAKDVVLVGKPEPKKEG"
     gene            5990..7648
                     /locus_tag="VBC28_000086"
     CDS             5990..7648
                     /locus_tag="VBC28_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003041886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HEO6814098.1"
                     /translation="MKLFTYRGIRVRKFHEKIKQLTFALFLLPFLLGGGILAYLHQNQ
                     LQNQFQRDPLTYGILFGGSVLGLLLLDGLVQVICYRNFLFFSRLDSLRILSHFLLDNN
                     LYKVNKIKSNHHTRERILLPKVYLKRDHFSITVSLHLQGSRFQDRFIALEKPLEIMFD
                     GDFMNKQFTKGYVSYTIAIDQFSGRLSIFDVKMTDKGIRLMKDVYWDFNKHPHLLIGG
                     GTGGGKTVLLMILIWVLAQIGYVEILDPKRSDFVGLKNIPVFKGRVYWDKKEMLACLK
                     RAEQEMDKRYDYMTSHPDYQAGKKYYAYGLKPRFIVIDELAALAAKLERDYQSSGDFI
                     EYLTELILKGRQSGVIMIVAMQRPDGEYLKTSLRDQFMKRISVGHLEDTGNKMMFGDA
                     NANKVFKKIDEIDGEEIFGRGYIANGGEVAREFYSPTVPFEEGFSFFEEYKKLPVLDD
                     PLLETTETTQTSSSDLSVEQAESPLLPLNDFAKEFDVPISTLRNLVKYIEEQDYTFRR
                     EDNRILLDEADRALLEEIVTEKEQADLPYKQVVLQHFETPPEPA"
     gene            7860..9092
                     /locus_tag="VBC28_000087"
     CDS             7860..9092
                     /locus_tag="VBC28_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEO6814099.1"
                     /translation="MISNTDLKRIRLELGFTQRKMATMIGYSYYNYRNIEQGQRKMTK
                     EFEEALFHFLNRKSETKLESTVDWLKIRFKTLDFKAVITHVLKLKLADFFYEEKSLYS
                     YSDMITYGSIRVLYSHSEKKAEAGTLIDLTGGGCRELELLLKQQGRNWFSFLHDVFLF
                     AEQERKDRPLEVFLAFPRFDIALDELYKKSGNLDLFDIKNRIFDNKIIMRKLRTFTAI
                     EGLKKVENRFVNQGLTLNFGSRQSNLMIRFYQKDYEQALLKDVSVDYIHEAYNYKNRY
                     EIELHDTKAFDILKEWYTLETDLTKIGARILNNYFEVKDWNGNYDSEWDNLLGTQAGF
                     KFVTRPRQINYARTKHWVTKQVSSALKLLKIVDMVYQTDELSEIISEAYLSNNHTKLA
                     EEICERNGVDFNVIVGQI"
     gene            9070..9456
                     /locus_tag="VBC28_000088"
     CDS             9070..9456
                     /locus_tag="VBC28_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002280079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6814100.1"
                     /translation="MSLSDRYKPFNVPDKFNRPLQTKTFPVGYEELYLSFYDFELVKD
                     LIDYWGLLYYQPKKDSELKYAEQFRKQAFKDENHQQNAIKKATRQEARQPFFEELKTK
                     PLKKMSQNARWVAEMLVQTGYAQLVL"
     gene            9476..9712
                     /locus_tag="VBC28_000089"
     CDS             9476..9712
                     /locus_tag="VBC28_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002270998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6814101.1"
                     /translation="MEYKVELNSLDNFRAWSGARNTLATVRERGDMDRLTSLGEDIFS
                     GSIPTETEINDWLWFDSDDIYRFLGYHDLVEDDV"
     gene            9709..10263
                     /locus_tag="VBC28_000090"
     CDS             9709..10263
                     /locus_tag="VBC28_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003034624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEO6814102.1"
                     /translation="MKLGKKIYEGISCFLDKNEFWNLYIVLMKEKEFFLDAFARKTID
                     LEYPAHYQHAYFTLDGQVLDFNQHMTTQLVTLFRQVILENQITFMEELIMATQNTLEK
                     KVRAVSLELGELMKAHDDKEAWKKAGELHGLLKKEEVKQLPEALVESLHAELRGYYYV
                     NSELNKLHKQLYAKGNKLIELANQ"
     gene            10273..11265
                     /locus_tag="VBC28_000091"
     CDS             10273..11265
                     /locus_tag="VBC28_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEO6814103.1"
                     /translation="MNTVKLIYHQLKTYLGNFTKRPKKKGKPTLTLANKKTVNLAVLS
                     GLAFILLVGLLGGIRAMTMSSKVTNLEKLIVAKKATSSPSSAYQTIDNRLQYYLNDFV
                     ECYFTIPADSDGQTKQAKQLTSFYGSEPDIQAQGQVKNPSQLSWSRLLTVTDNVATYE
                     VHYNQTVKNGDKTEEKELVTGFNIPYAKTKTGYYVTGLPWFSSLSTNQADKKSTDTEV
                     KLNQSDRVSEKTKKKLDKFLNVFFTNYTTDQDNLDLVAHNVTVVKNATFKTLDFSYYK
                     ATDKAVMAYVQVTFEVAGSTHAENFTLTLSEKGKSYYVEKVSHTIPANYADTKE"
     gene            11282..11509
                     /locus_tag="VBC28_000092"
     CDS             11282..11509
                     /locus_tag="VBC28_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017827907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6814104.1"
                     /translation="MSSSNLQTWLQGDGFWFITFGAIILIILAWRNSSWGRLFTTLVF
                     YAVIASLTGGGRQLLHVIGWFLRLFGIETGL"
     gene            11511..11927
                     /locus_tag="VBC28_000093"
     CDS             11511..11927
                     /locus_tag="VBC28_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003041913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEO6814105.1"
                     /translation="MEKERLYDYARGINAPYWIQEVKTQKGKRIWYFATPMQLSFFVV
                     FALVLILMLTVFSSLLLPLNKLTHSISCYLYWYIPYKLAKFYTEYEPQGKKMHVFLWD
                     YICYLMDFRLNRKAIYHEDRMKMVDEEEIVFEKTDL"
     gene            11938..14460
                     /locus_tag="VBC28_000094"
     CDS             11938..14460
                     /locus_tag="VBC28_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEO6814106.1"
                     /translation="MVLKLVYPILKTQDNIALTTDKMVMSFYRIPNTPITITDKAKKQ
                     KHKLTTAQLIKKLVKNKSFEVSLIPKDYLLEEKMKDFSHALSPDSQALGEELLQYSTD
                     KLTQEMEIPYQFDWLIGVNLLKDDQNTTVQDMAVERFSEVSEMIANSVGFQYEIPEDW
                     AQEYLVSEATVYQILSALRAQRLTNEELFYYQRMQYLRYISHLKQEVTANRALFNVTD
                     TVLRPMAGGFMKLDSPYGSSFLTILPIAKTPIIFNGFHLGEFTQRFNFPVELRIKAEF
                     IEKNSLKGRMARSNIRYRNIMNEAESTDTVQQDEIILGAMSLKDLMKKVGGKEEVIEY
                     GAYLIVSASSLSQLRRRRQVVLSYFEDMHVEVSEASHDTPYLFQSLLYGQPLENKTRT
                     WTHYVTPRGFAELMPFTNTASGNHIGWHIGRVDNWTGRWESIEKAQEASKNMVLFNAT
                     VGNKEGIAGKLTKNPHILITGATGEGKSFLAELIFLLTSLQDVKSLYVDPKRSIRKHF
                     EAIAHHPDFEKRFPLLAEHIKQINFVTLDSRVKSNHGALDPIVMLDKDDAVSVSKNML
                     NYLIFSNKRMKVTMKQMTAISRAINKVVAQRQTGEIVGLKHVLKELTNNPDSTIFEVG
                     DYLTSIVENSILELAFSDGNVEGLSYDKRVTVLEVADLSLPDSKDNQQDVTPDDKEIN
                     STALMFALGAFCTRFGELNRYEDTIEFFDEAWVLMKSAEGRAVIQSMRRVGRFFNNVL
                     CLITQSVHDAEGDDDTTGFGTLFAFREDNELPDILEHVGLTDNEENLEWIKNMISGQC
                     LYKDVYGNLNMISIYNIFDSIDPLLKPMKATVSSNLENKYAS"
     gene            14473..16404
                     /locus_tag="VBC28_000095"
     CDS             14473..16404
                     /locus_tag="VBC28_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002280085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEO6814107.1"
                     /translation="MQEAFEKLKGMDIFSLKSYMIKTGFGDFSGVWVMFNEIFVNFPF
                     FLLNLIVGFFSLMIRILENVRLYDTYKQYVFSGAQSIWRGFTGATSGVAQSSLVFLIL
                     LCLAFYLFYQLLMSKGNFSQRLLHVLLVLFLGFGWFGTVAGTSGGLYLLNTVDNVAST
                     TIHHISNVSVSYGKNQSLKIGSSVADSYIAETSYKAYLFVNTGQENGKYKDRQTGKEE
                     PFEDSKVLGSLDKSGKFKAVSTADRNKYLEIGNGASDDNEHNRWVSAIPDYIPVKFFY
                     ILFKMVEAIVIAIPIVLIQLLNVLAQALVLIMILLFPIALLVSFIPKMQDILFGVFKV
                     MFGGLAFPAITSLLTLALLYLEKIIENLVSSGFDKVIGDFSSLSTFSALFELIVSVCA
                     KGVIYWLLWKYKGELIELLLGSRAKITFDEIDTKVKETVTNGQEPLKQLPTKATNAFE
                     MAQTSGNFFLAGAGLGAGLFMNAGKQMKTLFSNPHPTSEGTEDSTPPENDEEETPTTS
                     SFEETGAPDEVTTPFQEPDMPMEIFESAIQEEDTSVQPFDTEPSPLMDTKQNREQKEF
                     EERRQNRLSWRQKHHINKLEKELEPYKDDEALYQAQGSNAFIKNYRKTLPKDDKLKAN
                     INRKHQIIEELARLRGGKE"
     gene            16445..16627
                     /locus_tag="VBC28_000096"
     CDS             16445..16627
                     /locus_tag="VBC28_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001882897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6814108.1"
                     /translation="MNNAVALAYKKRKPSIDFLLLFSVKEIEKEQLLATIKENPLILT
                     AQWRFETVMMTVYVKT"
     gene            16636..17691
                     /locus_tag="VBC28_000097"
     CDS             16636..17691
                     /locus_tag="VBC28_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEO6814109.1"
                     /translation="MKKKLVIVSALGALVLPLFLVVIIMGAFAGSTSSNGQAKGLQTK
                     LTAKEVAQKANISEERASDVIKILNYELSQEQFTLAGASGSLAVAERESGFDPKAVNT
                     GGGVAGYFQWSGWSSTVNGNRWSKAKQKKLDSDIELDLLSTELNGSYSKVKTEMQKAT
                     DPEEAALYWSEYYEGVALSDGQTKTSDLKKNARKWYNLFKDTLTGDTTNAKTRDGGGV
                     TSDGVPAGYSLTKAINTSNYTSATYPWGQCTWFVYNRAKEVGVNFDPYMGNGGDWKQK
                     AGYQTTHTPTEHSALSFSPEQAGADPTYGHIAFVEQVKSDGSILISETNTKGLGVVSY
                     RTFDKATANQFTYVIGK"
     gene            17748..18053
                     /locus_tag="VBC28_000098"
     CDS             17748..18053
                     /locus_tag="VBC28_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017827912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system protein"
                     /protein_id="HEO6814110.1"
                     /translation="MTKKMFQKRVDSELLDEVSVLYKSLGTSVGDAFVMFLQKSKEVN
                     GLPFDLRKQVHAISDNPLERLALANSNVIKVTASNPEAIADFFDEDYPEYEERHGQL"
     gene            18040..18372
                     /locus_tag="VBC28_000099"
     CDS             18040..18372
                     /locus_tag="VBC28_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000371058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEO6814111.1"
                     /translation="MDNYNPYDILVATIAFSDGTGAKRRPVLVIANNEEVIRTYRITS
                     QYSRKSSYIRSKYFEIKEWEEAGLVKPYWIDTVQLYDLDVELVQVRLIGKLTENDQQR
                     FEKFLREN"
     gene            18483..18755
                     /locus_tag="VBC28_000100"
     CDS             18483..18755
                     /locus_tag="VBC28_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO6814112.1"
                     /translation="MQVILPEEQTKEIQLFVANLIKNEIETVKEDSGQISPFLNKKQA
                     CQYLGISNNTLDIWIRKGMPHIRIGKTIRFDKDSIRRWMIQLENHF"
     gene            18810..20033
                     /locus_tag="VBC28_000101"
     CDS             18810..20033
                     /locus_tag="VBC28_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014619928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEO6814113.1"
                     /translation="MTITKTSNGSYRLKLYVPTEARPSLGISGNYYDKRFKTKKEAKE
                     AELQILIDINTIQKGGALPHQAINGDILFKDFYHNVWWEAYKAGQTTSTNKPPTLPTV
                     NNTETVFRLHILPMFGNYSIKFLNQNKTVVLNLMTVKANEYANFKVIRSYVNSIFDWA
                     EELEYIEMNRLSKTISRIKATKKIKLQEAKRDEDLYLTLSELREWFEMFQEDLENKKL
                     SLKDYVLFFTTFILNDRKSESYALQWKHISLDTSEIGLIQALDKYKKPKSTKGEKKTI
                     FAIPQELTVLLYQWKIQQKSELAKFGIIQTPEQYVFTYIDTKGNVNSPLHADYLNNKM
                     KSVERRHKNLRHATPHMLRHTGATLAKKAGMSLESISEALTHSDLSTTKIYVNTSNVV
                     PLSAGETAYRELKNK"
     gene            complement(20139..20531)
                     /gene="rpsI"
                     /locus_tag="VBC28_000102"
     CDS             complement(20139..20531)
                     /gene="rpsI"
                     /locus_tag="VBC28_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEO6814114.1"
                     /translation="MAQAQYAGTGRRKNAVARVRLVPGTGKITINKKDVEEYIPHADL
                     RLVINQPFAVTSTQGSYDVFVNVVGGGYAGQSGAIRHGISRALLEVDPDFRDSLKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(20552..20998)
                     /gene="rplM"
                     /locus_tag="VBC28_000103"
     CDS             complement(20552..20998)
                     /gene="rplM"
                     /locus_tag="VBC28_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEO6814115.1"
                     /translation="MNKTTFMAKPGQVERKWYVVDAADVPLGRLSAVVASVLRGKNKP
                     TFTPHTDTGDFVIVINAEKVKLTGKKASDKIYYTHSMYPGGLKQISAGELRSKNAVRL
                     IEKSVKGMLPHNTLGRAQGMKLKVFVGGEHTHAAQQPEVLDISGLI"
     gene            complement(21220..21342)
                     /locus_tag="VBC28_000104"
                     /pseudo
     CDS             complement(21220..21342)
                     /locus_tag="VBC28_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002278327.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(21879..22739)
                     /locus_tag="VBC28_000105"
     CDS             complement(21879..22739)
                     /locus_tag="VBC28_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEO6814116.1"
                     /translation="MTFKILTDSTSDLDEKWAQEHNVDIIGLTIELDGKTYETVGDEK
                     ITSDFLLERMQEGAKPTTSQINVGQFEEVFSTYAENDHALLYLALSSHLSGTYQSATI
                     AREMVLDKYPDAQIEIVDTMAASCGEGVLAMLATKERQEGKSLEEVKQKIESLLPKLN
                     TYFLVDDLNHLMRSGRLSKGAAIIGSVAKIKPLLKLDSEGKLVPFAKTRGRKKGIKEI
                     VTQATKTLSYSTLIIAYSGEKDSAQVMKEQLLADERIEEVIIRPLGPVISAHVGSGAL
                     ALFSLGEENR"
     gene            complement(22832..23350)
                     /locus_tag="VBC28_000106"
     CDS             complement(22832..23350)
                     /locus_tag="VBC28_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000716639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEO6814117.1"
                     /translation="MKKHSILLVDGYNMIAFWKDTRQLFKSNRLEEAREVLLRKLNHY
                     AHFEHIDIICVFDAQYVPGVRQRYDQYKISVIFTEEDETADSYIERAAAELNQSVLNL
                     VSVATSDLNEQWTIFSQGALRVSARELEQRVATVKSDLDKMSSQIDLSTPKLRPWNDE
                     QLGKLKDFLDGM"
     gene            complement(23347..24102)
                     /gene="rlmB"
                     /locus_tag="VBC28_000107"
     CDS             complement(23347..24102)
                     /gene="rlmB"
                     /locus_tag="VBC28_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEO6814118.1"
                     /translation="MTMKDKQFKEESSDLVYGLHAVTESLRANTGNKLYLQDDLRGKN
                     VDKVKALATEKKVSISWTPKKTLSDMTNGGVHQGFVLKVSEFAYADLSEIMTKAENEE
                     NPLILILDGLTDPHNLGSILRTADATNVTGIIIPKHRSVGVTPVVSKTSTGAVEHVPI
                     ARVTNLSQTLDTLKDKEFWIFGTDMNGTPSHKWNTKGKLALVIGNEGKGISHNIKKQV
                     DEMITIPMNGHVQSLNASVAAAILMYEVFRNRL"
     gene            complement(24205..24591)
                     /locus_tag="VBC28_000108"
     CDS             complement(24205..24591)
                     /locus_tag="VBC28_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HEO6814119.1"
                     /translation="MIDVRLINGIALAFEGDAVYSLYIRRHLIMQGFTKPNQLHRKAT
                     QYVSANAQALLINAMLEENILTDEEQLIYKRGRNANSHTKAKNADIITYRMSTGFEAL
                     MGYLDMTGQIKRLETLIQWCIETIEK"
     gene            complement(24584..25927)
                     /locus_tag="VBC28_000109"
     CDS             complement(24584..25927)
                     /locus_tag="VBC28_000109"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000591125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEO6814120.1"
                     /translation="MIKIYDTMTRSLQDFIPLNEGKVNMYVCGPTVYNYIHIGNARSV
                     VAFDTIRRYFEYCGYQVNYISNFTDVDDKIIKGAAEAGMDTKSFSDKFISAFMEDVAA
                     LGVKPATKNPRVIDYMDEIIDFVKVLVDKEFAYEANGDVYFRVSKSHHYAKLANKTLE
                     DLEIGASGRVDGEGEIKENPLDFALWKSAKSGEVSWESPWGKGRPGWHIECSVMATEI
                     LGDTIDIHGGGADLEFPHHTNEIAQSEAKTGKTFANYWMHNGFVNVDNEKMSKSLGNF
                     ITVHDMLKSVDGQVIRFFLATQQYRKPVNFTEKAVHDAEVNLKYLKNTFNLPIQENAN
                     DEELEQFVKAFQGAMDDDFNTANGITVIFEMAKWINSGHYTSRVKETFAELLEIFGIV
                     FQEEVLDADIESLIEQRQEARANRDFATADRIRDELAKQGIKLLDTKDGVRWTRD"
     gene            complement(25924..26106)
                     /locus_tag="VBC28_000110"
     CDS             complement(25924..26106)
                     /locus_tag="VBC28_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEO6814121.1"
                     /translation="MKDYVTTQILSILGILAAACLSYLKFAQGNPLLGIIWAAFVLMY
                     VVRLYKAYQKQKKEKP"
     gene            complement(26116..26700)
                     /gene="cysE"
                     /locus_tag="VBC28_000111"
     CDS             complement(26116..26700)
                     /gene="cysE"
                     /locus_tag="VBC28_000111"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000539954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEO6814122.1"
                     /translation="MGWWKESIAIVKEQDPAARSSLEVILTYPGIKALAAHRLSHFLW
                     NHNFKLLARMHSQFWRFWTQIEIHPGATISEGVFIDHGSGLVIGETAIVEKGAMLYHG
                     VTLGGTGKDKGKRHPTIRKGALISAHSQIIGPIEVGENAKVGAAAVVLADVPADVTVV
                     GVPAKVVRVHGQKDDLQIRSIEHDREESYYSSKL"
     gene            complement(26709..27461)
                     /locus_tag="VBC28_000112"
     CDS             complement(26709..27461)
                     /locus_tag="VBC28_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SseB family protein"
                     /protein_id="HEO6814123.1"
                     /translation="MTSTNELDIRLRAFINAPDNFLDSIGLVNALHHSTVWASKEPYA
                     IQVDGQEVVPVFTDITDLNHFKEEQESARDMFWESRRSLDVLDEAISHGLAGLVYNLK
                     KEGDFGNSTIFYCEDMVQFMNNYTTILNQLLNEDNIVADIMDKTYLVPAFVHPREEGS
                     FDRLFPTMSTPEGKSYVPVFSNLLSFEKWYNHNDFGGAFRKAQGVILAWTIDDIYKPR
                     NGENEIDDTFGVAINPFDEQQVLVDWSDVEND"
     gene            complement(27463..29592)
                     /gene="pnp"
                     /locus_tag="VBC28_000113"
     CDS             complement(27463..29592)
                     /gene="pnp"
                     /locus_tag="VBC28_000113"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEO6814124.1"
                     /translation="MSKQVFEMIFAGKKLVVETGQVAKQANGSVVVRYGDSTVLTAAV
                     MSKKMSTGDFFPLQVNYEEKMYAAGKFPGGFNKREGRPSTDATLTARLIDRPIRPMFA
                     EGFRNEVQVINTVLSFDENASAPMAAMFGSSLALSISDIPFNGPIAGVQVAYVDGNFI
                     INPTAQEQEASALELTVAGTKEAINMVESGAKELSEEIMLEALLKGHEAVCELIAFQE
                     EIVTAIGKEKAEVELLQVDPELQAEIIATHNIALQAAVQVEEKKAREAATEAVKEVVI
                     GEYEARYAEHEEYDRIMRDVAEILEQMEHAEVRRLITEDKIRPDGRRVDEIRPLDAEI
                     DFLPQVHGSGLFTRGQTQALSVLTLAPMGEAQIIDGLTPEYKKRFMHHYNFPQYSVGE
                     TGRYGAAGRREIGHGALGERALEQVLPSLEEFPYAIRLVAEVLESNGSSSQASICAGT
                     LALMAGGVPIKAPVAGIAMGLISDGTNYTVLTDIQGLEDHFGDMDFKVAGTREGITAL
                     QMDIKIEGITPQILEEALAQAKKARFEILDVLHGAIAEPRPQLAPTAPKIDMIKIDVD
                     KIKVVIGKGGETIDKIIAETGVKIDIDEEGNVSIFSSDQAAIDRTKDIIASLVREAKV
                     GEVYHAKVVRIEKFGAFVNLFDKTDALVHISEIAWTRTANVADVLEIGEEVDVKVIKI
                     DDKGRVDASMKALLPRPPKADNPKKES"
     gene            complement(29973..30242)
                     /gene="rpsO"
                     /locus_tag="VBC28_000114"
     CDS             complement(29973..30242)
                     /gene="rpsO"
                     /locus_tag="VBC28_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEO6814125.1"
                     /translation="MAISKEKKNEIIAQYARHEGDTGSVEVQVAVLTWEINHLNDHIK
                     QHKKDHATYRGLMKKIGHRRNLLAYLRRTDVNRYRELIQSLGLRR"
     gene            complement(30330..31589)
                     /locus_tag="VBC28_000115"
     CDS             complement(30330..31589)
                     /locus_tag="VBC28_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001203075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HEO6814126.1"
                     /translation="MRAWKGIVLILSSIVVTLVAWQNAGLSEFVVPGLALTSLSLTFL
                     LSTKFRILESYFQGIENMYFYHKVMAVFSMILLLLHKIGLGQGGHGSEFAKTIGSAGL
                     YLFLSIVFVAYFGNFLKYEIWRFIHRFVYLAYILGLVHTFMILGDRILGNTLLSLIVL
                     GYAVIGVISGFYIIFLYSRMRFRRVGYVQKVTHLNHDTTEIEIAMKRPYRYDYGQFTF
                     FKIYQAGFESAAHPFSISGGHDRVIFLTVKASGDYTKSIYKQLKVGTKIALDRAYGHM
                     LFDKDKKEQVWIAGGIGITPFISFIRENSILTKRVDFFYTFSNQDNLIYQDMLESYAK
                     ANPNFKLHLNNSSLKGRLDFSQSVFEGQPTIFMCGPTSMTSTYAKVFRQKDAKSRLVY
                     EGFSFRDSWLSIFLLKTFDKVYSNLIK"
     gene            complement(31698..32627)
                     /locus_tag="VBC28_000116"
     CDS             complement(31698..32627)
                     /locus_tag="VBC28_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001203818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HEO6814127.1"
                     /translation="MRCSTKEMRLVYRDFLLQANQENKQITVLEADLSSSMSTNALAS
                     EFGKRYINLGIMEAEMVGLAAGLAIKGYKPYLHTFGPFASRRVFDQVFLSLGYSQLSA
                     TIIGSDAGISAEMNGGTHMPFEELGLLRLIPKATIFEVSDDIQFEAILKQTLSIDGLK
                     YIRTIRKAPTAVYEGREDFSKGFIQLRQGKDITLVASGIMVSRAIEAADYLKELGIEA
                     SVIDLFKIKPLPEELKPLLIDQSIVTIENHNRIGGIGSALCEWLSMEKDTTVSRMGID
                     ERFGQVGQMEYLLEEYGLAVKDIVQHCKSIYKS"
     gene            complement(32624..33481)
                     /locus_tag="VBC28_000117"
     CDS             complement(32624..33481)
                     /locus_tag="VBC28_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEO6814128.1"
                     /translation="MTSPEERYSSLRRFATEIRLNTLETLNHLGFGHYGGSLSIVEAL
                     AVLYGDIMDINPEKFKESDRDYMVLSKGHAGPALYSTLYLKGFFDKTFLHSLNTNGTK
                     LPSHPDRNLTPGIDVTTGSLGQGISIATGIAYAQKIENSSYYTYTIVGDGELNEGQCW
                     EAIQFAAHHQLHHLIVFVDDNKKQLDGLTADICNPGDFVAKFEAFGFDAVRVKGDDIE
                     AIDKAIKTFQDSNSVRPKCIVLDSIKGQGVKELEELASNHHLRPDLQQKTMLERALIS
                     LRESLEVVE"
     gene            complement(33484..34839)
                     /locus_tag="VBC28_000118"
     CDS             complement(33484..34839)
                     /locus_tag="VBC28_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEO6814129.1"
                     /translation="MKGLLDFLVNIASTPAILVALIAIIGSVLQKKGVPDIVKGGIKT
                     FVGFLVVSGGTGIVQNSLNPFGKMFEHAFHLVGVVPNNEAIVAVALTKYGSATALIML
                     AGMIFNILIARFTKFKYIFLTGHHTLYMACMIAVIFAVAGFTSFSLILFGGLALGIIM
                     SVSPAFVQKYMIQLTGNDKVALGHFGSLGYWLSGFIGGIVGDKSKSTEDIKFPKSLSF
                     LRDSTVSITISMAIIYLIVAVFAGEAYIAKEISNGVNGLVYALQLAGQFAAGVFVILA
                     GVRLILGEIVPAFKGISEKLVPNSKPALDCPIVYPYAPNAVLIGFISSFVGGLVSMIV
                     MIVTGTTVILPGVVPHFFCGATAGVIGNASGGVRGATIGAFVQGILISFLPIFLMPVL
                     GGLGFKGSTFSDADFGLTGIILGALNHVGGAIAIVIGIVVILIGLFGISFVGKSTHKE
                     G"
     gene            complement(34852..35136)
                     /locus_tag="VBC28_000119"
     CDS             complement(34852..35136)
                     /locus_tag="VBC28_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008089859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEO6814130.1"
                     /translation="MLRIGTACGSGLGSSFMVQMNIESILKDLGVSDVEVEHYDLGGA
                     DPSAADVWIVGRDLEDSAGHLGDVRILNSIIDMDELRELVTGICQEKGLI"
     gene            complement(35139..37175)
                     /locus_tag="VBC28_000120"
     CDS             complement(35139..37175)
                     /locus_tag="VBC28_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEO6814131.1"
                     /translation="MVDNKTVVIMLVFLARKNLSLYELTVQTKFSIKVIIEQINYLNS
                     FLAKNHLPAIAHSAGRYQLLGDEKEHDKIVSLLEAEQFYLTQEERVCLIYLYSFCRRE
                     FVSNVHYQDFLKVSKNTTLSDIKMLRSKLAKRGISLTYTRAKGYSLVGDEMDKHQVAF
                     QMITQLLESPIGFWSLNYILSSWKFALSYEKLEKTVEYFYESFQLSPIQDRLEKSLYF
                     IILILCRYQRSVDRVLQGSPIVSEQLKELTTIIVTNLSQDISLSKPLDQKEKDYITLI
                     LSGCFEGEGTKDDDFFEALAKAIVDEMETVSLLNFSNKEELLQGLKRHIIPAYFRLKY
                     GLTGDSGYTQNIKEHYSDLFLLVKKALRPLEEQVGLIPDSEISYFVIHFGGYLRQSGG
                     TQSMSYKALILCPNGVSSSLVIKEKLRGLFPQIHFHRVSKIEQLKLIDNQTYDMVFST
                     IFVETKKPNYLVSLMMTAEQVQQLKELVISDFPKACLDDFQLDQLIATIKKYAHVHCE
                     EELKLALRTMVKQDILRKDVRPLLHQLITEETYQTSSEQMNWKEAIRLAAKPLLASGK
                     ITESYPEAMIEKVEEFGPFINLGKGIAIPHARPEDGVNSVGMSMLVLEQPIYLLDDPK
                     QEIYLLLCIAAIDNETHLKALSHLTTILRDNNNVKALLASRRYQDIEMIIKQED"
     gene            complement(37391..39016)
                     /gene="treC"
                     /locus_tag="VBC28_000121"
     CDS             complement(37391..39016)
                     /gene="treC"
                     /locus_tag="VBC28_000121"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HEO6814132.1"
                     /translation="MTIDKRKVVYQIYPKSYKDTTGNGVGDLRGIIEKLPYLAELGID
                     MVWLNPFYPSPQRDNGYDISDYTAINPDFGTMDDFEEMIEVGRQYRIDFMLDMVLNHC
                     SIEHEWFKKALAGDRYYQDFFILRDNPTDWVSKFGGNAWAPFGDTGKYYLHLFDITQA
                     DLNWRNADVRKELFKVVNFWRDKGVKGFRFDVINLIGKDEILENCPINDGKPAYTDRP
                     ITHDYLKMLNNASFGQDDSFMTVGEMSSTTIANCILYTAPEREELSMAFNFHHLKVDY
                     KDGQKWTIMAFDFPALRDLFHSWGEGMSEGNGWNALFYNNHDQPRALNRFVDVKRFRN
                     EGATMLAASIHLSRGTPYIYMGEEIGMLDPDYSSMDDYVDIESLNAYQIMLDEGKSQE
                     EAFSIIRAKSRDNSRVPMQWDDSTNAGFSEGAPWLKVGKSYKEINVAKEKTGLIFTFY
                     QELIRLRKQLPIIADGNYKAAFKDNEKVYAFERHLDKEKLLVLNNFFAEKVKIKLPEN
                     YLQGQVLLSNYKDVTLDETVTLQPYQTLAILVS"
     gene            complement(39238..41268)
                     /gene="treP"
                     /locus_tag="VBC28_000122"
     CDS             complement(39238..41268)
                     /gene="treP"
                     /locus_tag="VBC28_000122"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HEO6814133.1"
                     /translation="MEQFKHDAKALLEAIGGKENISAVTHCATRMRFVLNDSSKAKVK
                     VIEELPSVKGTFTNAGQFQVIIGNDVPIFYNAFVAVSGIEGVSKEAAKSAAQKNQNPL
                     QRVLTMLAEIFTPIIPAIIVGGLILGFRNILDAVPFEFLGQKVVDGVRQVDSSGHPIW
                     NTLVDVSTFWSGVDSFLWLPGEAIFHFLPVGIVWSVTRKMGTTQILGIVLGICLVSPQ
                     LLNAYSVASTSAADIAKNWSWNFGYFTVQKIGYQAQVIPALLAGLSLSYLEIFWRKHI
                     PEVVSMIFVPFLSLVPAIILAHTVLGPIGWTLGKWISAIVLIGLTGPVKWLFGAIFGA
                     LYAPFVITGLHHMTNAIDTQLIADTKTHTTGLWPMIALSNIAQGSAVLAYYFMHRHDE
                     KEAQISLPAAISAYLGVTEPALFGVNVKYIYPFVAGMIGSSVAGLLATTFNVQANSIG
                     VGGLPGFLSINVKYMGYFFICMAVAIFIPLFLTLFFKKSGILTKTEEEKLVPDAVIAS
                     TTETKSAKEKAVVSGTKLSVVSPLSGLAKPLDQASDPVFSQGIMGKGVVIDPSDGELV
                     SPVDATVSVLFPTKHAIGLLTSEGVEFLIHIGMDTVNLEGKGFTSHVAQGDTVKVGDK
                     LITFDIPMIKEEGYIVETPILITNQQEFRPEELIDLPKQIKRGQALMVAKKI"
     gene            complement(41551..42177)
                     /locus_tag="VBC28_000123"
     CDS             complement(41551..42177)
                     /locus_tag="VBC28_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001932323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6814134.1"
                     /translation="MTLEAKKLGFYHKKDQWLFKEINLEVAPGQVLGIFGQSGCGKTS
                     LSRVLAGFLHPKSGEVLVDGSNLPSKAFRPVQLIQQHPEKTMNPLWPMKKSLEEAYYP
                     SRDLLDAFGIQEKWLNRRPSELSGGELQRFSIVRSLHPETKYLIADEMTTMLDSITQA
                     SVWKSLLEIVKDRNLGLIVISHDFAMLEKLCNQCYMIEENRIVSFNGD"
     gene            complement(42161..42964)
                     /locus_tag="VBC28_000124"
     CDS             complement(42161..42964)
                     /locus_tag="VBC28_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6814135.1"
                     /translation="MTETLLSIKDLSITFTQYGRFLKPFQSTPIQALNLEIKKGELLA
                     IIGASGSGKSLLAHAIMDILPKNASVTGDMIYRGQSLNSKRIKQLRGKDITLIPQSVN
                     YLDPSTKVKHQVRLGISENSKATQEGLFQQFGLKESDGDLYPFQLSGGMLRRVLFTTC
                     ISDKVSLIIADEPTPGLHPDALQMVLDQLRSFADKGISVIFITHDIVAASQIADRITI
                     FKEGKAIETAPASFFSGNGEQLQTEFARSLWRSLPQQEFLKGVTHDLRG"
     gene            complement(42976..43797)
                     /locus_tag="VBC28_000125"
     CDS             complement(42976..43797)
                     /locus_tag="VBC28_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6814136.1"
                     /translation="MILRRRTIVLWQLGIAISLILSILALNLYFHSTPLQTNAALRNL
                     APSLNHLFGTDGLGRDMFVRTIKGLYFSLQVGLLGALMGVILATVFGVLAGLGNSIID
                     KIIAWLVDLFIGMPHLIFMILISFVVGKGAQGVIIATAVTHWPSLARLIRNEVYHLKN
                     KEFVQLSKSMGKTPYYIVRHHILPLIASQIFIGFILLFPHVILHEASMTFLGFGLSAE
                     QPSVGIILSEAAKHISLGNWWLVIFPGLYLILVVNAFDTIGESLKKLFYPQTDHF"
     gene            complement(43794..44771)
                     /locus_tag="VBC28_000126"
     CDS             complement(43794..44771)
                     /locus_tag="VBC28_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6814137.1"
                     /translation="MKHITTVVIWKILRCLSLVFGVSILTFILLKQSPVDPVMASVNY
                     DTSLTPVQYKAIAHHYGLDKPAPVQYFIWLKNFIQGHLGTSLVYRQPVIDIIRSRAGA
                     SFILMGLSWVLSGLIGFILGTLSAFHQGKLLDRIVRWFSYLQISVPTFWIGLIFLLIF
                     SVQLGWFPIGISSPIGTLSQDITLADRIKHLILPVFTLSILGIANVTLHTRTKMMSVL
                     SSEYVLFARARGETEWQIFKNHCLRNAIVPAITLHFSYFGELFGGSVLAEQVFSYPGL
                     GSTLTEAGLKSDTPLLLAIVMIGTLFVFAGNLIADILNSIINPQLRRKV"
     gene            complement(44884..46512)
                     /locus_tag="VBC28_000127"
     CDS             complement(44884..46512)
                     /locus_tag="VBC28_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEO6814138.1"
                     /translation="MTKYLKYISFVALFLASIFLVACQNQNSQTKERTRKQRPKDELV
                     VSMGAKLPHEFDPKDRYGIHNEGNITHSTLLKRSPELDIKGELAKKYKISKDGLTWSF
                     DLNDDFKFSNGEPVTADDVKFTYDMLKADGKAWDLTFIKNVEVVGKNQVNIHLTEAHS
                     TFTAQLTEIPIVPKKHYNDKYKSNPIGSGPYMVKEYKAGEQAIFVRNPYWHGKKPYFK
                     KWTWVLLDENTALAALESGDVDMIYATPELASKKVKGTRLLDIASNDVRGLSLPYVKK
                     GVVKNSPDGYPVGNDVTSDPAIRKALTIGLNRQKVLDTVLNGYGKPAYSIIDRTPFWN
                     PKTAIKDNKVAKAKQLLTKAGWKEQADGSRKKGNLKAEFDLYYPTNDQLRANLAVEVA
                     EQAKALGITIKLKASNWDEMATKSHDSALLYAGGRHHAQQFYESHYPSLAGKGWTNIT
                     FYNNPTVTKYLDKAMTSPDLDKANKYWKLAQWDGKTGASTLGDLPNVWLVSLNHTYIG
                     DKRINVGKQGVHSHGHDWSLLTNIAEWTWDESAK"
     gene            complement(46755..47483)
                     /gene="lrgB"
                     /locus_tag="VBC28_000128"
     CDS             complement(46755..47483)
                     /gene="lrgB"
                     /locus_tag="VBC28_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiholin-like protein LrgB"
                     /protein_id="HEO6814139.1"
                     /translation="MELLKTPIFGICFSLILYTIGEHLFKKSKGFFLLQPLFFAMVSG
                     IVILWLMSKGLGTDVKTFYTQAYKPGGDLIFWFLNPATIAFAVPLYKKNDVVKKYWVE
                     ILSSLVIGMIVSLILIVAISKMVGLSQVGIASMLPQAATTAIALPITAAIGGNTAVTA
                     MACILNAVIIYALGKKLVSFFHLNDSKIGAGLGLGTSGHTVGAAFALELGELQGAMAA
                     IAVVVIGLVVDLVIPIFSHLIGLL"
     gene            complement(47485..47940)
                     /locus_tag="VBC28_000129"
     CDS             complement(47485..47940)
                     /locus_tag="VBC28_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HEO6814140.1"
                     /translation="MKHTSKETKTKEAPMFIQMSIYAAILLVSQMISMLLPKSLPIPT
                     TVIGLVLMYVLLTAKIIKVEWVDSFGALMISMIGFMFVPSGISVAANLDILKAEGLQL
                     VAVITISTVVMLVVVAYVARLILAIRELSVTDLFKKILPNHYSKKVEEK"
     gene            complement(48110..48850)
                     /locus_tag="VBC28_000130"
     CDS             complement(48110..48850)
                     /locus_tag="VBC28_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HEO6814141.1"
                     /translation="MKILILDDEMFARQELSFLVEHSQEVDNPEIFQAEDISEAEKIL
                     FRQQIDLIFLDISLSEENGFTLANQLSQLAHPPLVVFATAYDNYAVKAFESNAVDYIM
                     KPFEQQRVDMALSKVKKLSQLTTASDVEQAIPKKASVELLTLTLSDRSVVVKMQDIVA
                     ASVEDGELTVSTVQKTYTIRKTLNWFKSRAVAPYFLQIHRNTVINLEMIEEIQPWFNH
                     TLLLIMSNGEKFPVGRSYLKDLNEHLTM"
     gene            complement(48831..50576)
                     /locus_tag="VBC28_000131"
     CDS             complement(48831..50576)
                     /locus_tag="VBC28_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000930326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor protein LytS"
                     /protein_id="HEO6814142.1"
                     /translation="MLMVLLFQRLGIIMILAFLLVNNSYFRQLIEERSKRETVVLVII
                     FGLFVIISNITGIEIKGDRSLVERPFLTTISHSDSLANTRTLVITTASLVGGPLVGSI
                     VGFIGGVHRFFQGSFSGSFYIVSSVLVGIVSGKIGDKLKENHLYPSTSQVILISIIAE
                     SIQMLFVGIFTGWELVKMIVIPMMILNSLGSTLFLAILKTYLSNESQLRAVQTRDVLE
                     LTRQTLPYLRQGLTPQSARSVCEIIKRHTNFDAVGLTDRSNVLAHIGVGHDHHIAGQP
                     VKTDLSKSVIFDGEPRIAQDKAAISCPDHNCQLNSAIVVPLKINDKTVGALKMYFAGD
                     KTMSEVEENLVLGLAQIFSGQLAMGITEEQNKLASMAEIKALQAQINPHFFFNAINTI
                     SALIRIDSDKARYALMQLSTFFRTSLQGGQDREVTLEQEKSHVDAYMNVEKLRFPDKY
                     QLSYDISAPEKMKLPPFGLQVLVENAVRHAFKERKTDNHILVQIKPDGHYYCVSVSDN
                     GQGISDTIIDKLGQETVAESKGTGTALVNLNNRLNLLYGSVSCLHFSSDKNGTKVWYR
                     IPNRIREDEHENFNS"
     gene            complement(50603..51247)
                     /locus_tag="VBC28_000132"
     CDS             complement(50603..51247)
                     /locus_tag="VBC28_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEO6814143.1"
                     /translation="MEKVIIFDMDGVIVDSEYTFLDNKTEMLREEGIDTDVSYQYQYM
                     GTTFEFMWQAMKEEFGLPKTVKEYIAEMNRRRQAIVARDGVRPIKGAQRLIHWLHQHG
                     YRLAVASSSPMVDIKRNLKELGVTECFEYMVTGEDVSSSKPAPDVFLRAAELLDVDPK
                     VCIVIEDTRNGSLAAKAAGMYCFGFANPDYPPQDLSMADKVISDYQDIYIYLPE"
     gene            complement(51371..51766)
                     /locus_tag="VBC28_000133"
     CDS             complement(51371..51766)
                     /locus_tag="VBC28_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEO6814144.1"
                     /translation="MYNKVIMIGRLTAKPEMVKTPTDKSVTRATVAVNRRFKGSNGER
                     EADFINVVMWGRLAETLASYGTKGSLISIDGELRTRKYEKDGQTHYITEVLASSFQLL
                     ESRAQRAMRENNVSGDLSDLVLEEEELPF"
     gene            51847..52563
                     /locus_tag="VBC28_000134"
     CDS             51847..52563
                     /locus_tag="VBC28_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000186175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEO6814145.1"
                     /translation="MTNVTSYKEMLAKPWGKIQYEITFAQLSHIKNQNVLDFGAGFCL
                     TEQHLAKENNVTAIEPNPKLLYDNQSDNIYKILGSYEALRDLPDQSFDTIICHNVLEY
                     IDKHNHPAYFDEFSRLLKPNGELSLIKHNITGKILQSVIFSNDTSTAMELLTGEANFK
                     SASFDQGNIYTLEELKQNTNLLVERYQGVRTFYSLQPNHFKTETGWLNKMLAIELSVA
                     DKAPYKDIAFLQHITLKKSL"
     gene            complement(52617..53243)
                     /locus_tag="VBC28_000135"
     CDS             complement(52617..53243)
                     /locus_tag="VBC28_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 and tRNA-binding domain-containing
                     protein"
                     /protein_id="HEO6814146.1"
                     /translation="MIFTYNREHVGDTLMVIVKDSQGAKLDVDRRGQVARVYLQDSKE
                     TVAWNIFEVSSLIVIEGAGQITLSDQDIKILNAELLKEGFEDSLVNNIEPTFVVAQIK
                     EMIDHPDSDHLHICQAEINDGKTVQIVCGAPNASVGLKTVAALPGAMMPNGSLIFPGK
                     LRGEDSFGMLCSARELALPNAPQVRGIIELSDQVIVGESFDANKHWKN"
     gene            complement(53276..53599)
                     /locus_tag="VBC28_000136"
     CDS             complement(53276..53599)
                     /locus_tag="VBC28_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEO6814147.1"
                     /translation="MILPESYEEIAAYIDSTKKVVFFFTADWCPDCQFIYPVMPSIEK
                     DFSDFVFVRVNRDDYIELAQQWNIFGIPSFVVVENGQELGRLVNKNRKTKAEITKFLA
                     EINYK"
     gene            complement(53596..53880)
                     /locus_tag="VBC28_000137"
     CDS             complement(53596..53880)
                     /locus_tag="VBC28_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4651 domain-containing protein"
                     /protein_id="HEO6814148.1"
                     /translation="MKNKKILFGTGLAGVGLLAAAGYTLTKKVTDYKRQQITQTLREF
                     FSQMGDIQVFYFNEFESDIKMTSGGLVLEDGRIFEFIYRQGVLDYVEVSK"
     gene            54041..54280
                     /locus_tag="VBC28_000138"
     CDS             54041..54280
                     /locus_tag="VBC28_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6814149.1"
                     /translation="MKEKLLVPALNCLLGLLFLGDHWFLFRLQNGELFAIVTLILLCT
                     TSFLINKNHPKNLTLIAMVITFLAVLSFLSTFLLA"
     gene            54465..55532
                     /gene="pepA"
                     /locus_tag="VBC28_000139"
     CDS             54465..55532
                     /gene="pepA"
                     /locus_tag="VBC28_000139"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HEO6814150.1"
                     /translation="MSDLFNKIKTVTELDGIAGYEHNIRNFLRQEITPLVDQVETDGL
                     GGIFGVKNTHETNAPKVMVAAHMDEVGFMVSHIQPDGTFRVLEVGGWNPLVVSSQRFT
                     LYTRSGDAIPVISGSVPPHFLRGQSGGTTLPKISDIVFDGGFTDKNEAESFGIAPGDI
                     IVPKSETILTANQKHIMSKAWDNRYGVLMVTELLKSLKDQSLSNTLIAGANVQEEVGL
                     RGAHVSTTKFNPDIFLAVDCSPAGDIYGEQGKIGEGTLIRFYDPGHIMLKDMRDFLLT
                     TAEEAGIKYQYYAANGGTDAGAAHLKNSGIPSTTIGVCARYIHSHQTLYAMDDFLQAQ
                     AYLQAIVNKLDRSTVDIIKGY"
     gene            55602..56372
                     /locus_tag="VBC28_000140"
     CDS             55602..56372
                     /locus_tag="VBC28_000140"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEO6814151.1"
                     /translation="MKIGIIGVGKMASAIIQGLKQTQHDIIISGSCLERSKEIAERLD
                     VTYAESHQSLINQADIIMLGIKPQLFEKVLLPLDITKPIISMAAGISLARLSQLTRSD
                     LPLIRIMPNINAQILQSCTAICYNNHVSDELRQLAKEITDSFGSSFDIAETNFDTFTA
                     LAGSSPAYIYLFIEALAKAGVKYGFPKEQALSIVGQTVLASSQNLLQGQNSTSDLIDN
                     ICSPGGTTIAGLLDLEKNGLTHSVISAIDATIEKAKKL"
     gene            complement(56393..57058)
                     /locus_tag="VBC28_000141"
     CDS             complement(56393..57058)
                     /locus_tag="VBC28_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEO6814152.1"
                     /translation="MSFIIRKGKILALLIAFLVINQLVPILAVWLLKNHYQTPFTSIL
                     LIGLELLIIALFLYYAKVKQIIRWKALLTRKALVTILLGWLSLRVPQIIGYLIMTMQG
                     VKDTANQTVIMELTKQLPLALMLIFAIIGAPIMEEIIFRYIIPKELFAKHQKWGFVIG
                     TLAFALIHSPSDIGSFIIYAGMGAILSFVYYKTEHLEYSIMIHFINNALAYSVLISTM
                     MNN"
     gene            complement(57127..57582)
                     /locus_tag="VBC28_000142"
     CDS             complement(57127..57582)
                     /locus_tag="VBC28_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6814153.1"
                     /translation="MLIKGLLSPNSKVSPKDYKVYMRVLSFVFSVCSLIMIVSSFLHT
                     FESNFTKGFSFGSGIALFAGSFYFLVITFSEKRFQHYYTKVFDERNQFIANMSCRLLF
                     LLSVLVLVVLCLINVWFGITFSYAKFLLLILSFILYGYLLIYFILQKLL"
     gene            complement(57623..57760)
                     /locus_tag="VBC28_000143"
     CDS             complement(57623..57760)
                     /locus_tag="VBC28_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001867090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6814154.1"
                     /translation="MSRMWTIDFCPPIVYQQYVFIITISISIAMSIFAIYRYASLKKT
                     I"
     gene            complement(57819..58025)
                     /locus_tag="VBC28_000144"
     CDS             complement(57819..58025)
                     /locus_tag="VBC28_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO6814155.1"
                     /translation="MKNSLQKLRKSRKLSQAELAVALGVTRQTIISLEKEKYTASLEL
                     AFKIARYFDKQIEEVFIYTESEGG"
     gene            complement(58177..59370)
                     /locus_tag="VBC28_000145"
     CDS             complement(58177..59370)
                     /locus_tag="VBC28_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000047536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEO6814156.1"
                     /translation="MSKTIAINAGSSSLKWQLYEMPEEKVVAKGIIERIGLKDSISTV
                     KFDDKKDEQILDIVDHTQAVKILLEDLTKHGIIKDFNEITGVGHRVVAGGEYFKESAL
                     VDDKVVEQVEELSALAPLHNPAAAAGIRAFREILPDITSVCVFDTAFHTTMQPHTYLY
                     PIPQKYYTDYKVRKYGAHGTSHQYVAQEAAKQLGRPLEELKLITAHVGNGVSITANYH
                     GQSIDTSMGFTPLAGPMMGTRSGDIDPAIIPYLVANDPELEDAAAVVNMLNKQSGLLG
                     VSGTSSDMRDIEAGLQSKDPNAVLAYNVFIDRIKKFIGQYLAVLNGADAIIFTAGMGE
                     NAPLMRQDVIAGLSWFGIELDPEKNVFGYFGDITKPDSKVKVLVIPTDEELMIARDVE
                     RLKAK"
     gene            complement(59402..60376)
                     /locus_tag="VBC28_000146"
     CDS             complement(59402..60376)
                     /locus_tag="VBC28_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEO6814157.1"
                     /translation="MNFEKIETAYELILENIQTIENQLKTHIYDALIEQNSYYLGSSC
                     DLDMVVVNNQKLRQLDLSQEEWRRTFQFIFIKSAQTEQLQANHQFTPDSIGFILLFLL
                     EELTSQETVDVLEIGSGTGNLAQTLLNNSSKELNYMGIEVDDLLIDLSASIAEIIGSS
                     AQFIQEDAVRPQILKESDVIISDLPVGYYPNDGIAKRYAVSSSKEHTYAHHLLMEQSL
                     KYLKKDGIAIFLAPENLLTSPQSDLLKEWLKGYADVIAVLTLPETIFGSRQNAKSIFV
                     LKKQAEQKPETFVYPLTDLQNRENMANFIENFQKWSRENSHYSKNMIK"
     gene            complement(60491..60862)
                     /locus_tag="VBC28_000147"
     CDS             complement(60491..60862)
                     /locus_tag="VBC28_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000601101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGG"
                     /protein_id="HEO6814158.1"
                     /translation="MILKKKLKAGILLQAIVLAAVFTLVLQFYLARILATERQYHSQI
                     EASKAYLTAQLAYKTIEGDSISGKCYFTGGYASYLQEGNYLQVKVTLDKGGNYNHKFY
                     RKTDTLQVPLKSNDNKASHKH"
     gene            complement(60840..61295)
                     /locus_tag="VBC28_000148"
     CDS             complement(60840..61295)
                     /locus_tag="VBC28_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HEO6814159.1"
                     /translation="MKNLLLKCKDKKVKAFTLLECLVALVTITGALLVYQGLTKLLAQ
                     QIVVMSSSSQSEWVLLTQQLNAEFEGAHLEYLRQNKLYLRKQDKIVTFGKSNKDDFRK
                     TGYDGRGYQPMVYGLDNCQMSQTKSMVKLVFYFKDGLKRTFYYDFKEET"
     gene            complement(61292..62383)
                     /locus_tag="VBC28_000149"
     CDS             complement(61292..62383)
                     /locus_tag="VBC28_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011074699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEO6814160.1"
                     /translation="MTLRQVILKNTHQTSGIDKWISWLKKDISVRNRHKSKKLSLKKQ
                     RKVVQLFNNLFASGFSLTDMVTFLKRSKLLSDCYTDSMNKALLEGKDLSKMLGELGFS
                     DTVITQVALADLHGNISRSLLKIESYLANLLLVRKKVIEVATYPLILLSFLVLIMIGL
                     RNYLMPQLGENNFATRLITNVPNIFLLLLAVVLIFSLIFYIIQKRLSRIKVACFLTTI
                     PLVGSYVKLYLTAYYAREWGNLLSQGIELDQIVKVMQNQKSKLFREIGYDMEEGFLSG
                     KAFHQKVLDYPFFLTELSLMIEYGQVKAKLGTELDIYADEKWEDFFTKLARATQLIQP
                     VIFIFVALIIVMIYAAMLLPMYQNMEILS"
     gene            complement(62268..63200)
                     /gene="tadA"
                     /locus_tag="VBC28_000150"
                     /pseudo
     CDS             complement(62268..63200)
                     /gene="tadA"
                     /locus_tag="VBC28_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062917.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
     gene            complement(63373..63738)
                     /locus_tag="VBC28_000151"
     CDS             complement(63373..63738)
                     /locus_tag="VBC28_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HEO6814161.1"
                     /translation="MYQVVKMFGDWEPWWFIEGWEEDITEIAEYDTLSEALLYFQEEW
                     DRGQEKWPYFQSKSSLLATFWSIKEKRWCEECDEYLQQYHSLMLLKEWQEIPKEESIE
                     RFEVFNKIAELPSACSLNL"
     gene            complement(63852..67502)
                     /gene="rpoC"
                     /locus_tag="VBC28_000152"
     CDS             complement(63852..67502)
                     /gene="rpoC"
                     /locus_tag="VBC28_000152"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEO6814162.1"
                     /translation="MVDVNRFKSMQITLASPSKVRSWSYGEVKKPETINYRTLKPERE
                     GLFDEVIFGPTKDWECACGKYKRIRYKGIICDRCGVEVTRAKVRRERMGHIELKAPVS
                     HIWYFKGIPSRMGLTLDMSPRALEEVIYFAAYVVIDPMDTPLEPKSLLTEREYREKLQ
                     EYGYGSFVAKMGAEAIQDLLKRVDLDAEIAVLKEELKSATGQKRVKAVRRLDVLDAFK
                     KSGNKPEWMVLNILPVIPPDLRPMVQLDGGRFAASDLNDLYRRVINRNNRLARLLELN
                     APGIIVQNEKRMLQEAVDALIDNGRRGRPITGPGSRPLKSLSHMLKGKQGRFRQNLLG
                     KRVDFSGRSVIAVGPTLKMYQCGVPREMAIELFKPFVMREIVARDLAGNVKAAKRMVE
                     RGDERIWDILEEVIKEHPVLLNRAPTLHRLGIQAFEPVLIDGKALRLHPLVCEAYNAD
                     FDGDQMAIHVPLSEEAQAEARLLMLAAEHILNPKDGKPVVTPSQDMVLGNYYLTMEDA
                     GREGEGMIFKDHDEAVMAYQNGYVHLHTRVGIAVDSMPNKPWTEEQKHKIMVTTVGKI
                     LFNDIMPEDLPYLIEPNNANLTEKTPDKYFLEPGQDIQAVIDNLEINIPFKKKNLGNI
                     IAETFKRFRTTETSAFLDRLKDLGYYHSTLAGLTVGIADIPVIDNKAEIIDAAHHRVE
                     DINKAFRRGLMTEEDRYVAVTTTWREAKEALEKRLIETQDPKNPIVMMMDSGARGNIS
                     NFSQLAGMRGLMAAPNGRIMELPILSNFREGLSVLEMFFSTHGARKGMTDTALKTADS
                     GYLTRRLVDVAQDVIIREDDCGTDRGLTITAITDGKEVTETLEERLIGRYTKKSIKHP
                     ETGEILVGADTLITEDMAAKVVKAGVEEVTIRSVFTCNTRHGVCRHCYGINLATGDAV
                     EVGEAVGTIAAQSIGEPGTQLTMRTFHTGGVASNTDITQGLPRIQEIFEARNPKGEAV
                     ITEVKGEVVAIEEDSSTRTKKVFVKGQTGEGEYVVPFTARMKVEVGDEVARGAALTEG
                     SIQPKRLLEVRDTLSVETYLLAEVQKVYRSQGVEIGDKHVEVMVRQMLRKVRVMDPGD
                     TDLLPGTLMDISDFTDANKDIVISGGIPATSRPVLMGITKASLETNSFLSAASFQETT
                     RVLTDAAIRGKKDHLLGLKENVIIGKIIPAGTGMARYRNIEPLAVNEVEIIEGTPVDA
                     EVTEVSTPTTED"
     gene            complement(67619..71194)
                     /gene="rpoB"
                     /locus_tag="VBC28_000153"
     CDS             complement(67619..71194)
                     /gene="rpoB"
                     /locus_tag="VBC28_000153"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEO6814163.1"
                     /translation="MAGHEVQYGKHRTRRSFSRIKEVLDLPNLIEIQTDSFQDFLDAG
                     LKEVFEDVLPISNFTDTMDLEFVGYELKEPKYTLEEARIHDASYSAPIFVTFRLVNKE
                     TGEIKTQEVFFGDFPIMTEMGTFIINGGERIIVSQLVRSPGVYFNDKVDKNGKVGYGS
                     TVIPNRGAWLELETDAKDIAYTRIDRTRKIPFTTLVRALGFSGDDEIVDIFGDSELVR
                     NTIEKDIHKNPSDSRTDEALKEIYERLRPGEPKTADSSRSLLVARFFDPRRYDLAAVG
                     RYKINKKLNLKTRLLNQTIAENLVDGETGEILVEAGTVMTRDVIDSIAEHIDGDLNKF
                     VYTPNDYAVVTEPVILQKFKVVAPTDPDRVVTIVGNSNPEDKVRALTPADILAEMSYF
                     LNLAEGIGKVDDIDHLGNRRIRAVGELLANQFRIGLARMERNVRERMSVQDNEVLTPQ
                     QIINIRPVTAAVKEFFGSSQLSQFMDQHNPLSELSHKRRLSALGPGGLTRDRAGYEVR
                     DVHYTHYGRMCPIETPEGPNIGLINNLSSFGHLNKYGFIQTPYRKVDRSTGAVTNEIV
                     WLTADEEDEFTVAQANSKLNEDGTFAEEIVMGRHQGNNQEFPSSIVDFVDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLIDPKAPYVGTGMEYQAAHDSGAAVIAKH
                     DGRVIFSDAEKVEVRREDGSLDVYHVQKFRRSNSGTAYNQRTLVKVGDLVEKGDFIAD
                     GPSMENGEMALGQNPVVAYMTWEGYNFEDAVIMSERLVKEDVYTSVHLEEFESETRDT
                     KLGPEEITREIPNVGEDSLRDLDEMGIIRIGAEVKEGDILVGKVTPKGEKDLSAEERL
                     LHAIFGDKSREVRDTSLRVPHGGDGVVRDVKIFTRANGDELQSGVNMLVRVYIAQKRK
                     IKVGDKMAGRHGNKGVVSRIVPVEDMPYLPDGTPVDIMLNPLGVPSRMNIGQVMELHL
                     GMAARNLGIHIATPVFDGASSEDLWETVQEAGMDSDAKTVLYDGRTGEPFDNRVSVGV
                     MYMIKLHHMVDDKLHARSVGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGASNVL
                     QEILTYKSDDVTGRLKAYEAITKGKPIPKPGVPESFRVLVKELQSLGLDMRVLDEDDN
                     EVELRDLDEGEDDDVMHVDDLEKARVKQEAEEKQAEQVSEVVQED"
     gene            complement(71718..74015)
                     /locus_tag="VBC28_000154"
     CDS             complement(71718..74015)
                     /locus_tag="VBC28_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEO6814164.1"
                     /translation="MFKGNKKLNSSKLGDYTPLEFGSIFLRIVKLLSDFIYVIILLFV
                     MLGVGLAVGYLASQVDSVKVPSKNSLVTQVNTLTRVSRLTYSDKSQISEIATDLQRTP
                     VAKDAISDNIKKAIIATEDENFNDHKGVVPKAVLRAAAGSVLGFGESSGGSTLTQQLL
                     KQQILGDDPSFKRKSKEIIYALALERYMDKDSILSDYLNVSPFGRNNKGQNIAGIEEA
                     AQGIFGVSAKDLTIPQAAFLAGLPQSPIVYSPYTADAQLKSDKDLSFGIKRQKNVLYN
                     MYRTRALTKDEYKSYKDYDIKKDFIKPAVATTNHHDYLYYSALSEAQKVMYNYLIKKD
                     NVSEHDLKNDETRATYRHRAIEEIQQGGYTIKTTINKSVYQAMQDAAAQYGGLLDDGT
                     GKVQMGNVLTDNSSGAIIGFIGGRNYSENQNNHAFDTARSPGSSIKPILPYGIAIDQG
                     MLGSGSVLSNYPTTYSSGEKIMHADEEGTAMVNLQESLDISWNIPAFWTYKMLRDRGV
                     DVKNYMEKLDYPIENFGIESLPLGGGIDTSVAQQTNLYQMIANGGVYHKQYMIESIED
                     SNGKVIYNHESKPVRVFSKATATILQQLLHGPINSGKTTTFKNRLQGLNSGLAGVDWI
                     GKTGTTNSTSDVWLMLSTPKVTLGGWAGHDNNASLAKLTGYNNNANYMAHLVNAINNA
                     DGNTFGKSERFRLDDSVIKAKVLKSTGLQPGVVTVNGRRITVGGESTTSYWAKNGPGT
                     MTYRFAIGGTDSDYQKAWSTLGGKR"
     gene            74126..75385
                     /locus_tag="VBC28_000155"
     CDS             74126..75385
                     /locus_tag="VBC28_000155"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEO6814165.1"
                     /translation="MNIFDELKERGLVFQTTDEDALRKALEEGSVSYYTGYDPTADSL
                     HLGHLVAILTSRRLQLAGHKPYALVGGATGLIGDPSFKDVERSLQTKKTVVSWGNKIR
                     GQLSNFLEFETGDNKAVLVNNYDWFSNISFIDFLRDVGKYFTVNYMMSKESVKKRIET
                     GISYTEFAYQIMQGYDFYELNKNYNVTLQIGGSDQWGNMTAGTELIRRKSNGVSHVMT
                     VPLITDSTGKKFGKSEGNAVWLDADKTSPYEMYQFWLNVMDADAVRFLKIFTFLSLKE
                     IEDIRIQFEEAPHQRLAQKTLAREVVTLVHGEKAYKEAVNITEQLFAGNIKGLSVKEL
                     KQGLRGVPNYHVQTEDNLNIIDLLVTSGVVNSKRQAREDVSNGAIYINGDRIQDLEYT
                     ISENDKLENEITVIRRGKKKYFVLNFK"
BASE COUNT        24354 a        14884 c        12765 g        23458 t
ORIGIN      
        1 acttttcaat gaaattctga tatactacta aagtaataaa tacataattc gaggtaaatt
       61 atgccctatg caatagactt attctgtggt gctggcggat tcagtgaagg aattctacaa
      121 gctggatttg atattatctt ctctagcgat aaaagcccaa tggtagaaga aacttataca
      181 aaccgacata aacaactagg tttagtagaa ggtgttgata cacactttga actcgcagat
      241 ataaaagaat taacttctga taaaattttt caatctatta actcattaaa atacggaaat
      301 atttttgaaa gagggactat tgatgtcatt tttggtggtc ctccttgtca aggattcagt
      361 cgtttaggaa aaagagattc caaagaccct agaaatatgc tctttcatga atatttgcgt
      421 attataaaag atgttatgcc acgttatgtt gttatggaaa atgtaactgg tattttagat
      481 atgcagatgt tagacttccc tagcgtactc aatgatagaa tctacggggg acaacatttg
      541 gttcagtcca ttcttagaaa tgagctggaa gaattaggat atactttact tgatgtgcag
      601 gttcttaatg ctgcaaactt tggtgtccca caacaaagaa atcgtgttgt atttctagca
      661 taccgtaatg acgtagcacc cattcaatat ccagaaagtg ataatgatat tccaaacgct
      721 actgtctatg acgctttagg agacctttat ggtggggaat ttagctatga aacaaattat
      781 tccctacaaa gtcgactggg taggactcct aatactgatg gtcaccccat tacaaatgat
      841 aatccgttga atatggaaat ctcaagacac gatgatattg tcacacagcg attctcacta
      901 tatcaacaag gagaaaatag agctaaagcc gttaatagaa tccaattaga aggactagaa
      961 ttaaaaaggg actatccaaa cctatttatt gaaagtctat ttcaaatcaa tggtaaagct
     1021 aaccagcttc acattatcaa gcaactaaaa aaacaggatt tgcttcaaga aagttttaaa
     1081 tccgagagat ggttacaatt taccaatcgt cagcttgggt tattatcaat gaacgatacg
     1141 gataactctg ctaaacaaca tattttaaaa tcattagcgc ttagattaaa aacttcattt
     1201 gaggaggctg aactattttg gaatgaaatc aagaaccagt tgaattcaga tattacggaa
     1261 gataagtttt caaaaatgct aagagatgga gatttaactc ctgaagcaat cgaagccatc
     1321 tttactaaaa agagtattcg agttcgtctt gaccaaaata gtgtctcacc aactatggta
     1381 acattaccag atgactatat ccacccattc tttaaccgtg ttttaactgt tagggaaatg
     1441 gctcggctgc aatcttttga tgatagtttt gaatttcttg ggaagcgtac cactggtgga
     1501 gataaacggg caaaagagac accacaattt actcaagtag gtaatgctgt cccaccatta
     1561 ctggctaagg caattgctga acaggtgatg attgctctaa acaatgatta aaaagctttt
     1621 tatatactcc aaatatttca ttttgaatta tgtctgtaac atttttctag tataatacta
     1681 taaaaggagg tttggaaaat gaaaaaaata cttagtctat tacttttagg aattactgct
     1741 atatttctaa tatcttgttc caaacaaaat agcctttatg gaaaatatta tgatatctat
     1801 gacggtaaag ctaaacttat tttagagttt aatgaaaatg gtgggagatt ttacgagaat
     1861 gaaacccgtg ctataacgaa tattgatact aaaaataaga cttttacatt ttctgttaac
     1921 ggtcgtgacg ttgttgttac ctacgattta aaagaaaatg gcactttaaa gtatgataca
     1981 ggtagttatt ttacttcagg taatgaaaat attgcataca aaaaggatag tgaagcctat
     2041 aaaaaggcac tgaaaaaatg acttagagat tttctctagg tcattttctt gttattaaaa
     2101 gaagttttgc atgtcgttta gcttctactt ctgtcacttg tttgggattc caagcatagc
     2161 taatagattg ccccttatca tacaatcccc agattttgaa ctttggataa cattgtttta
     2221 tcaaagaggt gaattgagta tctgctaact ctggtaagtc aatatctgaa atgaagtttg
     2281 attgatttat taaatagttg aacttatcta atgcgctagg ttcaaattca ttgtaatcag
     2341 agatgatata gtctttcact tggtcaaagt agccgacaat gtgtttgttt tttctagtta
     2401 ggtaatcctc tacaatctgt tttgcggata gtctattgat gtctaattca aaatttaaga
     2461 agtctgtcaa acgagttttg agagcttgac cagaaacttg tagtgctgct tctacctctt
     2521 gaaaagccat ttgctggtaa aaaatcttag ttagaagcac aatatcaggc attaagagaa
     2581 aagctgagta aaaattagcc tctctttctt gtagagaatc atcatgaaaa tcagtgaata
     2641 ctcgcccact gtgatgtaat aagatgtgcc ctaattcatg acattttgta aaattttgac
     2701 gagtaataga atcactatct tgataggaaa tcgttaaaac atcatcattg atattaaaac
     2761 ctgctatatc tatccggata tggtgaggaa taacaagtat atttttcttg tcaacttcat
     2821 aattaaagta atcctgaaaa gtataataaa gaggatgtac tttatttaat ttcatgaatg
     2881 gataaagtgt ttgtttgaca cgattccaaa cattaaagta aattctttgc acgaaacatc
     2941 agtctttctt ttctttattc atagccctaa tggctttttc tctctctaac agaaattctc
     3001 tcaaatcttc ttctagtatt tttttctctt catcagataa atttttagaa gttttccgat
     3061 atagaacttc aaatgttgat aaatcaatct catctgaatt tttgtagtca gatttaccca
     3121 ttaaataatc aatggataca ttgtaaaaat tcgctaattt agtaataact gattgtgttg
     3181 ggttagcttt agcattttcc catttagcat aggcttgctg agaaatccca aattcttttg
     3241 ctacatcgct ttgtgttaga ttggcttcta aacgcagagc ttttaagcgt tcaggaaaca
     3301 tttttgtacc tctttaaaaa aaattaattt ttttacaaaa aactcttgac aatacaacct
     3361 ataagttgta ttatatattt acaacctgaa ggttgtgtaa gtcttgttaa tactaaacct
     3421 aaaattatga aatagataaa tttaataaag tatgaaaaag gtcgatatta acaaaaagat
     3481 ttaaatttgt cctttgaaaa ttgaataaaa aagtgcgtca gacaacctca ctcaagctta
     3541 gctgaccaac acacaataat catttagcaa ttattattgt acaggtttta tcgcaaaaag
     3601 tcaaggaggt tggagtagat gaacaaaaaa atcctttgga gttatctcac gcactcggct
     3661 ttttgcccct cttgaccgga taacaaaggg gaactgctgg cgctggtggc gtcggaaatg
     3721 ctgacaggta agatacgttc tgctggggta agcataggcg gttaatcgtg gctatagtga
     3781 agtgagatga ctataataac agagattagt caagtacagc tatatagagc ggtgataaca
     3841 acactgagtg tgcgacgata cgagcacgcc ctaaacagag gattcatctg tttagacgag
     3901 tagaaaacac ttaaaaaaat acaaatgatt gacaatgtaa actatatgga ttacaataaa
     3961 taatgaaagg tggtggcatt tatggcaact gtaccaactc aaattcggat tgatgaaact
     4021 gtaaaagccc aagcaacttc attatttaat gacttgggaa tggatatgtc tagtgcggtc
     4081 aatatttttc ttcgtcaatg tctattgcgt ggtggacttc ctttcacagt tgaagttcct
     4141 aattatagtc aaaaaacctt agaagctctg gcagaagcta aacgaatctc tcgtgataaa
     4201 acgatcgctt cttatgacaa tctagcagat ttaaagaagg ctctggacgc ttagatgtat
     4261 caagtcaagt tcacctctac ttttaaaaag agctataaaa ggattaaaaa gcgaggctta
     4321 gagatgacac tcttagatga agtgattgaa aaactccgtc taggtcagac actcgaagaa
     4381 aaataccgtg accatgaact caaaggaaac tttgttggct ttagggaatg tcatattaaa
     4441 ccagattggc tcttgattta tctgattgaa gacgatattt tgactttaac cttagccgac
     4501 acaggcagtc atgcggattt gtttaagatg tgaagacagt tctttgtgag ctgtcttttt
     4561 tgttttctac tcctttaagc tgatgaatca ttagcctatc catgtccttt caaacggaca
     4621 gttcaaaaaa ttgccattct aaaggaaagc gaggaaaaaa gatgaatgat tatgaacaaa
     4681 gttttcgccg tatttatgag aactttgttt tttctttgaa aatctatccg gataagcatt
     4741 atcaaaaggt cttatgttat caagtgctag aaaaaatgga taagttgttt cacgactacc
     4801 aaaaactagg cttgccaaca gttcaattag tccaatggaa aaatcactac aaatttaaag
     4861 ttcaacatga ttacattatg aaaggaggta caacatgaat tattttgaac aacgattcca
     4921 acaaatctat gagaagtttc ttttctccct taaaatttat catgctaatc ctacacattg
     4981 tgaaacatgc taccgagact gtctgaatga aatggatagt ctttttttac gccatgatac
     5041 tcatgaccag tttgctaaac agcttttgaa ttgtaaaaag acttttcaat ttaaagttaa
     5101 aaaagcctat tttggaatgt aaggaggacg gtatgaccag agtaagagca cctgccgatt
     5161 aaaaagatga gttgattttt tgacctagac aagtcataaa acggtctaaa caaaatagaa
     5221 attgaggtaa acataatgga aattacaaaa gtattcgttg accaaaacca agtattagga
     5281 aatgaagtct ttgtatcagg tatggaagaa gtccgtgcca atgattacaa ccctgtctct
     5341 tatcgtatct ttgaaatttc aagtcgaaaa gagccctctg ccttcctcat tgtagaaggt
     5401 tttaaaatgt ctgatgtccc agaaggtcgt caaaaagtcg tcttccctcg tggaattgcc
     5461 attgaacgcc gtaacttccg ctttggaggc aaacgtaaac atgaaaatgt cattgttgct
     5521 gaagaaatgc gtacagtaaa gtctaaataa gaaatagaat aggagaaaca ctatgactgt
     5581 aaaatttgct gctgatgtga ttgaaacttt tgatactgta aaaactcttg gaactctaaa
     5641 tttccttgaa gctattccct ttatgcactg ggaagactat gtggaagaag gaactggtga
     5701 agaaaagcgt cgtgaaacag ataaggttga ttatgtagat gtgaaactct actcctctgc
     5761 ggtcaatggg gatattaccg taaccgttcc acctgaagca aaagtggctc aaatgaccgc
     5821 tgaaaagaac tacaatgatg aagtaacgct tgtagctcct actgctcgtt tttggtcaaa
     5881 tgcagaaacc attaacaatc gccgtatcac cacaagcggg gttaaactac gggctaaaga
     5941 cgttgtatta gtggggaaac ctgaacctaa aaaggaagga tagtagccta tgaaactgtt
     6001 cacctatcgg ggaatccgtg ttcggaagtt tcatgagaaa atcaaacaac taacttttgc
     6061 tctttttctt ctgccctttc tccttggagg gggtatttta gcttacctgc accaaaacca
     6121 gcttcaaaac cagtttcaac gtgacccgct aacctacggg attctttttg gtggaagtgt
     6181 attaggatta ttgctactag acggcttagt acaggtgatt tgttaccgaa attttctttt
     6241 ctttagccgt ctagatagtt tacggattct ttcccacttt ttacttgata ataacttgta
     6301 taaggtcaat aaaatcaaat cgaatcatca cacgagagaa cggattctat tacccaaagt
     6361 gtatttgaag cgtgaccatt ttagtattac cgtttcactc caccttcaag gcagccgatt
     6421 ccaagaccgc tttattgccc ttgaaaagcc tcttgaaatt atgtttgacg gagacttcat
     6481 gaacaagcaa tttaccaaag gctacgtttc ttataccatt gccattgacc aattttctgg
     6541 tcgcttatct atctttgatg ttaagatgac agataaggga atccgcctaa tgaaagatgt
     6601 ctattgggat ttcaataaac acccacactt gctgattggt ggtggaacgg gtggcggtaa
     6661 aacggttctc ttgatgattt tgatttgggt cttagctcaa attggttatg tggaaattct
     6721 tgaccctaaa cgctctgact ttgtcggatt gaaaaatatt cctgtcttta aaggtcgggt
     6781 ttactgggat aaaaaagaaa tgctagcctg tttaaaacgt gctgagcaag aaatggataa
     6841 acgctatgat tacatgacca gccaccctga ttatcaagca gggaaaaagt attatgctta
     6901 cgggttaaaa cctcgtttta tcgtcattga tgagttggca gccttagcgg ctaaactaga
     6961 gcgagattac caatcatctg gagactttat cgaataccta acagaattga ttttaaaagg
     7021 gcgccaatcc ggagtcatca tgattgttgc tatgcaacgt cctgacgggg aatacctcaa
     7081 aacgtcttta cgtgaccaat ttatgaaacg gatttctgtt ggtcacttag aagatactgg
     7141 taataaaatg atgtttgggg acgctaacgc aaataaagtt ttcaaaaaga ttgatgaaat
     7201 tgacggagag gagattttcg gtcgtggtta tattgcaaac ggtggggagg tggctagaga
     7261 attttattca cctacagttc cctttgagga aggcttttct ttctttgagg aatacaagaa
     7321 actacccgtc ttagatgacc cactattaga aacaacagaa actactcaaa cgtcatcatc
     7381 tgacttgtcc gttgaacaag cagaatctcc cctccttcct ctgaatgact ttgccaaaga
     7441 atttgatgtg cccatttcaa ccttacgaaa ccttgtgaaa tacatagaag aacaggacta
     7501 tacattcaga agagaggata accgtattct gctagatgaa gcagataggg ctttgttgga
     7561 ggaaattgta acagagaaag aacaggctga cttgccctat aagcaagttg tccttcaaca
     7621 ctttgaaaca cctcctgagc cagcgtaggc acgaaggtca aggtcagggc tacgcccgtc
     7681 cgacctgccc gaccgagctg acaagcgggc gttttggggt gtgccctagg cacgccccaa
     7741 aagggcttaa cctcccgata ctaatagggg ggttaaaact aacacaagtc cttaaaaggc
     7801 tgttagattc cctattcttc ccgtcactag aacaaacaca aaaaggaggt ggaaaaagag
     7861 tgataagcaa taccgattta aagcgtatca ggcttgagtt aggatttact caacgcaaaa
     7921 tggcaactat gattggttat agttactaca actaccgaaa cattgaacaa ggtcaacgaa
     7981 aaatgaccaa agagtttgaa gaagcactct ttcactttct gaatcgcaaa tcagaaacca
     8041 agctagaaag cacggtggat tggttaaaaa ttcgcttcaa aacacttgat tttaaagctg
     8101 tcatcacaca cgttctaaaa ttaaaactag ctgacttttt ttatgaagaa aaaagtctat
     8161 acagctattc agatatgata acctatggtt caattcgggt actttactcc cattctgaaa
     8221 agaaggcaga agctggaacg ctgattgact taacaggagg gggttgtcgt gagcttgaat
     8281 tgttattaaa acaacaaggt cggaattggt tttcttttct tcacgatgtc tttctctttg
     8341 cggaacaaga acgaaaagat agacctttgg aagttttttt agcctttcca cgctttgata
     8401 ttgcccttga tgaattatac aaaaaatcag ggaatcttga cttatttgat attaaaaatc
     8461 ggatttttga caataaaatc atcatgcgca aactccgcac ctttaccgct attgaaggct
     8521 tgaaaaaagt tgaaaatcgc tttgtcaatc aaggtttgac cttaaacttt ggcagtcgtc
     8581 aatccaatct catgattcgc ttttatcaaa aggattatga acaagccctc ttaaaagacg
     8641 tttctgttga ctatatccat gaagcttata attataaaaa ccgttatgag attgaactac
     8701 atgataccaa ggcgtttgat attttaaaag aatggtatac gttggaaact gacttgacta
     8761 aaataggcgc tcggatttta aataactatt ttgaagtgaa agattggaat ggcaactatg
     8821 atagtgaatg ggataacctg cttggcacac aagcgggttt taaatttgtc acacgtcctc
     8881 gtcaaattaa ctatgcaagg acaaaacatt gggttacaaa acaagtctct agtgctttga
     8941 aattgctgaa aatcgttgat atggtttatc agacagatga gctttcagaa atcatttctg
     9001 aagcctattt gagtaacaat catacgaaat tagcagaaga aatttgtgaa cgaaatggag
     9061 ttgattttaa tgtcattgtc ggacagatat aaaccattca atgttcccga taaattcaat
     9121 cgccctcttc aaacaaagac cttcccagtc ggttatgaag aactctacct atcgttttac
     9181 gattttgaat tggtcaaaga tttgattgat tattggggac tcttatatta tcaacctaaa
     9241 aaagattctg aattgaaata tgcagaacaa tttagaaagc aagcctttaa agatgaaaac
     9301 caccaacaaa atgcgattaa aaaggcgact aggcaagaag ctagacaacc tttttttgaa
     9361 gagttaaaaa caaaaccact aaaaaagatg agtcaaaacg ctcgttgggt ggcagaaatg
     9421 ctcgtacaga cgggctatgc tcaattggta ctataaaaat gaaaggaaaa atgaaatgga
     9481 atataaagtt gaattgaata gtctggacaa tttcagggct tggtcaggtg cacgcaatac
     9541 actagcaact gtccgtgaac ggggagatat ggacagacta acctcacttg gtgaagatat
     9601 tttttcagga agtatcccta cagaaacaga aatcaatgat tggctttggt ttgattctga
     9661 tgacatctat cgattccttg gctatcatga tttggtagaa gatgacgtat gaagcttgga
     9721 aaaaaaattt acgagggaat ctcctgtttc cttgataaaa acgaattctg gaatctctac
     9781 attgtcctaa tgaaagaaaa agagtttttc ttagacgctt ttgcacggaa aaccattgac
     9841 cttgaatacc ccgcccacta ccaacatgct tatttcacac tggatggtca agtgttagac
     9901 ttcaatcaac acatgacgac acaacttgtc actcttttta gacaggttat cttagaaaat
     9961 caaataacat ttatggagga actgataatg gcaacacaaa acacacttga aaagaaagtc
    10021 agagcggtct cattggagct aggagaattg atgaaagccc atgatgataa agaagcttgg
    10081 aaaaaagcag gtgaattgca cggacttctc aaaaaagaag aggtgaaaca actccctgaa
    10141 gcactggttg aaagtcttca cgctgaattg cgaggttact attatgtcaa tagtgaactt
    10201 aataaacttc ataaacaact ttatgcgaaa ggaaataaat tgattgaact agccaatcag
    10261 taaggggtac ctatgaatac agtaaaactt atctatcatc aattaaaaac ttatcttgga
    10321 aattttacca aacgtcctaa aaagaaagga aaaccaacat taacccttgc caacaaaaaa
    10381 acagtcaatc ttgccgttct atcaggattg gcttttattt tgcttgtagg gctactagga
    10441 ggcattcgtg cgatgacaat gtctagtaaa gtcaccaatc ttgaaaaact gatagttgct
    10501 aaaaaagcta cttcttctcc ctcttctgct taccaaacaa tagataatcg cctgcaatat
    10561 tatctgaatg attttgtaga gtgctatttc acgattcctg cagattcaga cggtcaaacc
    10621 aaacaagcta agcaattaac gagcttttac ggttcagagc ctgatattca agcacaaggt
    10681 caagtcaaaa atcctagtca attgagttgg tcacgcctgc taaccgttac agataatgtg
    10741 gcaacgtatg aggtgcatta caatcaaacg gtaaaaaatg gggataagac agaagaaaaa
    10801 gaattggtaa cgggttttaa tatcccttat gccaaaacaa aaacgggtta ctatgttacg
    10861 ggcttacctt ggttttctag tttatcgacc aatcaggcag ataagaaaag taccgataca
    10921 gaagtcaagc tcaatcagtc agatagggtt tcagagaaaa caaaaaagaa attggataag
    10981 tttctcaacg ttttcttcac taactatacg actgaccaag ataatttgga cttagtggct
    11041 cacaatgtaa cagttgtaaa aaatgccact tttaaaacct tagattttag ttactacaaa
    11101 gcaaccgata aagcagttat ggcttatgtt caagtaacat ttgaagtcgc aggctcgaca
    11161 catgctgaaa acttcacctt gaccttatca gaaaaaggga agtcctatta tgtggagaaa
    11221 gtcagccata caatccctgc taattacgca gatacaaaag aataaaagga gagtttaatc
    11281 aatgagttca tctaatttac aaacatggtt acaaggagac ggtttctggt tcattacctt
    11341 tggagcgatt atcctgatta ttttagcttg gagaaacagc tcttgggggc gactatttac
    11401 cacgcttgtt ttctacgcag tgattgcctc tttaacagga ggcggacgac aattacttca
    11461 cgttatcggt tggttcctcc gtctctttgg aattgaaacg gggttgtgac atggaaaaag
    11521 aacgattgta tgattacgct agagggataa acgctcccta ctggattcaa gaagtaaaaa
    11581 cacaaaaagg aaaacgtatt tggtattttg caacacccat gcaactttcc ttttttgtgg
    11641 tttttgcact ggtcttgatt ttaatgttga cagtgtttag ctcacttctt cttccgttga
    11701 acaaattaac gcattccatt tcatgttatc tttattggta tatcccttat aaactcgcta
    11761 aattttatac agagtatgaa ccacaaggaa agaaaatgca cgttttctta tgggattata
    11821 tctgctatct tatggatttt cgcctcaatc gtaaagcgat ttatcatgaa gatagaatga
    11881 aaatggtgga tgaagaagaa attgtttttg aaaaaacaga tttataaagg aggtctaatg
    11941 gttctaaaac ttgtctatcc gattcttaaa acacaggata atatagcttt aacaacagat
    12001 aaaatggtga tgagttttta tcgtattccc aatactccca tcaccattac agataaagca
    12061 aagaaacaaa aacacaagct gacaactgct cagctgataa aaaaactggt taaaaacaag
    12121 tcctttgaag tctccttgat tccaaaggac tatttgttag aagaaaagat gaaagacttt
    12181 tctcatgcct tgtctcccga tagtcaagca ttgggggaag aactgttgca gtattcgaca
    12241 gacaaattga cacaagaaat ggaaattccc tatcaatttg actggttgat tggtgtcaat
    12301 cttttaaaag atgaccaaaa caccaccgtt caggatatgg cggttgagcg cttctctgaa
    12361 gtctctgaaa tgattgccaa tagcgtgggc tttcaatatg agattcctga agattgggcg
    12421 caagagtatc tggttagtga agcgaccgtc tatcaaatcc tttccgctct tcgggcacaa
    12481 cgtttgacca atgaagagct cttttactat caacgcatgc agtacttgcg ctatatttct
    12541 catctaaagc aagaagtcac agccaatcgg gcactcttta atgtgactga tacggtctta
    12601 agaccaatgg ctgggggctt tatgaagtta gacagcccct atggttcttc ttttctcacc
    12661 attcttccga ttgctaaaac acccattatt tttaatggct ttcatctagg ggaatttacc
    12721 caacgcttta atttccctgt agaactacgg ataaaggcag aatttatcga aaagaactct
    12781 ttaaaagggc gaatggcacg ttctaacatt cgttatcgaa atattatgaa tgaagctgaa
    12841 agtacagata ctgtccaaca agatgaaatt attctggggg caatgtctct caaagacttg
    12901 atgaaaaagg ttggtgggaa agaagaagtc attgaatatg gagcttatct cattgtttct
    12961 gcttctagcc tatcccaatt acgacgtcgc cgtcaggtag tcttaagtta ctttgaggat
    13021 atgcacgtag aagtgagtga agctagtcat gatacgcctt atctcttcca gtctctcctc
    13081 tatggtcaac cacttgaaaa taagaccaga acgtggacac actatgtcac tccaagaggt
    13141 tttgcagaac taatgccatt taccaacaca gcttcaggaa accatattgg ctggcatatc
    13201 ggacgggtgg ataattggac gggacggtgg gaaagtattg aaaaggcaca agaagcgtct
    13261 aaaaatatgg tgctctttaa tgcgaccgtt ggaaataaag aagggattgc aggaaaactt
    13321 acaaagaatc cgcatatcct aattactggc gcaacgggtg aaggaaaatc ttttttggca
    13381 gaacttatct ttctcctaac ctctcttcaa gatgtgaaat ctttgtatgt tgacccgaaa
    13441 cgctctatcc gaaaacactt tgaagcaatt gctcaccacc cagactttga aaaacggttc
    13501 ccgcttctgg ctgaacatat caaacagata aactttgtca ctttggatag tagggtgaaa
    13561 tccaatcatg gggctcttga cccaattgtc atgttggata aagatgacgc tgtttcagtt
    13621 tcaaaaaaca tgctcaatta tcttattttc tccaacaagc gaatgaaggt cacaatgaag
    13681 caaatgaccg ctatttcgag agcaattaac aaagtggtgg ctcaaagaca aacgggagaa
    13741 atcgtggggt taaaacatgt tctcaaagag ctcaccaata atccagatag cactatcttt
    13801 gaagtaggag attatttgac gtccattgtc gaaaattcca ttttggaatt ggctttttca
    13861 gacggtaatg tagaaggatt gtcttatgat aaacgggtga ctgttttaga ggtagcggac
    13921 ttatccttac cagatagtaa agataatcaa caagatgtca ccccagacga taaagaaatc
    13981 aattctacgg cgctgatgtt tgctttggga gctttctgta cacgctttgg agaactcaat
    14041 cgttatgaag ataccattga atttttcgac gaagcgtggg tactgatgaa gtcggcagaa
    14101 ggacgagccg ttatccaatc catgcgtcgt gttggacgtt tctttaataa tgtcctctgc
    14161 ctcattactc aatccgttca tgacgctgaa ggagatgatg ataccacagg ctttgggaca
    14221 ctctttgcct ttagagaaga caatgaactt cccgatattt tagaacacgt tggactaacg
    14281 gataatgaag aaaatctgga atggattaaa aatatgattt cagggcaatg cctctataaa
    14341 gatgtttacg gcaatctcaa tatgatttcc atttataaca tttttgatag tatcgacccg
    14401 cttctaaaac caatgaaagc tactgtttct tctaatcttg aaaataaata cgcatcttaa
    14461 ggaggtgagc ttgtgcaaga agcatttgaa aaattaaaag gcatggatat tttttcttta
    14521 aaatcctaca tgataaaaac tggctttgga gatttcagcg gtgtttgggt gatgttcaac
    14581 gaaatctttg tcaacttccc ttttttcctc ctcaacctca ttgttggttt cttctctttg
    14641 atgattcgga ttttagaaaa cgtccgactt tatgacactt acaagcaata tgtcttcagt
    14701 ggagctcaat ccatttggcg aggctttaca ggagctactt ctggtgtggc tcagtcatca
    14761 ttagtctttc tgattctcct ttgcttggct ttttatctct tttatcagct cctcatgtct
    14821 aaaggcaatt tctctcaaag attgcttcat gtccttctgg ttctcttttt aggctttggt
    14881 tggtttggga cagttgctgg aacttctggt ggattgtatc tgctcaatac agtagacaat
    14941 gtcgctagta ctacgattca tcacatttct aatgtctccg tctcctacgg aaaaaatcag
    15001 tctttaaaaa tagggtcttc cgtagctgat tcttatatcg cagaaacctc ttataaagcc
    15061 tatctctttg tcaatacagg acaagaaaac ggaaaataca aagaccgtca aacaggaaaa
    15121 gaagaaccgt ttgaggattc aaaagttcta ggaagcttgg ataaatcagg caagtttaaa
    15181 gccgtttcta ccgctgatag gaataaatat ttagaaatag gaaatggagc aagtgatgat
    15241 aatgaacata accgttgggt atcggctatt cctgattata tccctgttaa gttcttctat
    15301 atccttttta aaatggttga agcgattgtc attgccattc ctattgtctt aattcagttg
    15361 ctcaatgtcc ttgcacaagc ccttgtcctt atcatgattc ttctttttcc tattgccctt
    15421 ttggtttcct ttattcccaa aatgcaagat attctttttg gagtttttaa ggtcatgttt
    15481 ggtggtcttg ctttcccagc cattacaagc ctgttaactt tggctttact ttaccttgaa
    15541 aaaatcattg aaaacttagt aagtagtggc tttgataagg ttattggaga tttttcttcc
    15601 ttgtctactt ttagcgctct ctttgaacta atcgtctctg tttgtgctaa aggagtgatt
    15661 tattggttgc tttggaaata caaaggagaa ttgattgaat tgttactcgg ttcacgagca
    15721 aagattacgt ttgatgagat agataccaaa gtcaaagaaa ccgttacaaa tgggcaggaa
    15781 cccctgaagc agctgcctac aaaagctact aacgcctttg aaatggctca aacatccgga
    15841 aactttttcc ttgctggagc gggattggga gcggggctct tcatgaatgc tggtaaacag
    15901 atgaaaactc ttttttcaaa tccccatccg acttcagaag gaacggaaga ttcaacgcct
    15961 ccagaaaatg acgaggagga aacacccact acttcttcct ttgaagaaac aggcgctccc
    16021 gacgaggtaa caacaccgtt tcaagagcca gatatgccaa tggagatttt tgaatctgct
    16081 attcaggaag aagacacaag cgttcaacct tttgacacag aaccttcccc gctaatggat
    16141 acaaagcaga acagagagca gaaagagttt gaagagcgcc gtcaaaatcg cctttcttgg
    16201 agacaaaaac atcacatcaa taaacttgaa aaggaattag aaccatacaa agatgatgaa
    16261 gcactatacc aagcgcaagg ttcaaatgcc tttatcaaga actatcgtaa aaccttgccg
    16321 aaagacgata aattgaaagc caatattaac cgtaaacatc agataataga ggaattagca
    16381 cgattgagag gaggaaagga ataatgaata tacgcattta ttctaaaaga ggttttaaca
    16441 gacggtgaac aatgcggttg cccttgccta taaaaagaga aagccttcta ttgactttct
    16501 ccttttattt tcagtaaaag agattgagaa agagcaactg ttagcaacta tcaaagaaaa
    16561 tcctcttatt ttgacggctc aatggcggtt tgaaacggta atgatgactg tctatgtgaa
    16621 aacttaggag ggaagatgaa gaaaaaacta gtaattgtta gtgctttagg tgctctggtc
    16681 ttacctttgt tcttggttgt tattatcatg ggagcttttg cgggtagtac gtcttctaat
    16741 ggacaagcca aaggactaca aaccaaacta acggctaaag aagtagctca aaaagccaat
    16801 atttcagaag aacgggcaag tgatgtcatc aaaattctca actatgaact ctcacaagaa
    16861 caatttactc tcgcaggagc ttctggaagt ttagcggttg cagaacgtga aagtggcttt
    16921 gaccctaaag ccgtcaatac aggtggcggt gtggctggtt atttccagtg gtcaggctgg
    16981 tcttctactg tcaatggcaa tcgctggtca aaggcaaaac aaaagaaatt agattctgat
    17041 attgaactag acctcttatc tacagagcta aatggctctt atagcaaagt caaaacagaa
    17101 atgcaaaaag cgactgaccc tgaagaagca gctctttatt ggtcagaata ttatgaaggg
    17161 gtcgctttat cagacggtca aaccaaaact agtgacctga agaagaatgc gagaaaatgg
    17221 tataacctct tcaaagacac tttaacaggg gatacaacta atgccaaaac acgagacggt
    17281 ggaggcgtaa cttctgatgg agtgcctgct ggatatagcc ttacaaaggc aatcaacact
    17341 tctaactata cttctgcgac ctatccttgg ggacaatgca cgtggtttgt ctataaccga
    17401 gccaaagaag taggcgttaa ctttgaccct tacatgggca atggtgggga ttggaagcag
    17461 aaagcaggct accaaaccac acatacacca actgaacata gtgctctcag cttttctcct
    17521 gaacaagctg gcgcagaccc aacttacgga cacattgcgt ttgtggagca ggtcaaatca
    17581 gacgggtcta ttctcatttc agaaaccaac accaaaggac ttggagttgt ttcttaccgc
    17641 acatttgata aagcaacagc taaccaattt acatacgtaa ttggaaagta aaaatttgac
    17701 aaaaaactac atttgtcgta caattgaagt acaaaagagg tgattgtatg actaaaaaaa
    17761 tgtttcaaaa gcgagtagat tcagaattat tagatgaagt atcggtacta tacaaatcct
    17821 taggaacttc tgtaggtgac gctttcgtga tgttcttaca aaaatcaaag gaggtgaatg
    17881 gattgccatt tgatttaagg aaacaagtcc atgctatttc tgataatcct ttagaaagac
    17941 tagctttggc aaactcaaat gttatcaaag tgactgcttc taatccagaa gcaatcgcag
    18001 atttctttga tgaagactat ccagaatacg aggaaagaca tggacaacta taacccatat
    18061 gatattctgg tagcaactat cgcttttagt gatgggacag gagctaaaag acgtcctgtt
    18121 ctagtcattg caaataatga agaagttatc agaacctacc gtatcactag tcagtattcc
    18181 cgtaaatcat cttatatccg ttcaaaatac tttgaaataa aagaatggga agaggcagga
    18241 ttagtgaaac catattggat tgataccgtt caactttatg atttagacgt agaacttgtt
    18301 caagttcgct taatcggaaa gttaacagaa aatgaccaac agagatttga aaaattttta
    18361 agagaaaatt aagattatac gattatatta taaataatcc taaaactttt ctttttcata
    18421 atctccttaa ggtaagggct ttatagctct tgctttttta ttgcctaaaa gggaggaaaa
    18481 ttatgcaagt tattttacca gaagaacaaa caaaagaaat tcaacttttt gttgcaaatc
    18541 ttattaaaaa tgagattgaa accgttaaag aagattcagg acaaattagt ccttttttaa
    18601 ataaaaagca ggcctgtcag tatctgggaa tttcaaataa cacgctggat atctggataa
    18661 gaaagggaat gccacatatt cggattggaa aaacaattcg atttgataaa gacagtatcc
    18721 gtagatggat gatacaactg gaaaatcact tttgatttca ttataataaa gtcagggtaa
    18781 ttttctagct cagtaaaaag gagattacta tgacaattac aaaaacctca aatggttctt
    18841 atcgtttaaa actctatgtt ccaacagaag ctagaccttc tttgggaatt tcaggaaact
    18901 actacgataa gcgtttcaaa acaaagaagg aggctaaaga agctgaacta caaattttaa
    18961 tagatattaa cactatccaa aaagggggtg cattaccaca tcaagcaatc aatggtgata
    19021 ttctcttcaa agatttttat cataatgtct ggtgggaagc ctataaagca ggtcaaacga
    19081 cttcaacaaa taaaccaccc actcttccaa cagttaacaa tacagaaact gtttttagat
    19141 tacatatttt accaatgttt ggaaattatt ccattaaatt tttaaatcag aataaaacgg
    19201 ttgttttaaa tttgatgact gttaaagcaa atgaatatgc caatttcaag gtcatacgta
    19261 gttatgttaa ttctattttt gactgggcag aggaattaga atatattgaa atgaatcgcc
    19321 tatctaaaac aatcagtcgt attaaagcga ctaaaaaaat caaattacaa gaagctaagc
    19381 gtgatgaaga tttatacctt actttaagtg agttacggga atggtttgaa atgttccaag
    19441 aagacctaga aaacaaaaaa ttgtcgctta aagactatgt tttatttttt acaactttta
    19501 tcttaaacga ccgtaaatcg gaaagctatg ctttacagtg gaaacatatc tccttagata
    19561 catctgaaat tggtttaatt caagccttgg ataaatataa gaagcctaag tctactaaag
    19621 gagaaaagaa aacaattttt gctattcctc aagaactaac tgttctctta tatcaatgga
    19681 aaattcaaca aaaatcagaa ttagcaaaat ttggtatcat tcaaactcca gaacaatatg
    19741 tatttactta tattgacact aaaggcaatg tgaatagccc tctgcatgca gattacctta
    19801 ataacaagat gaaatctgta gaacgtcgcc ataaaaatct taggcatgcc actcctcaca
    19861 tgttaaggca tactggtgct actttagcta agaaggcagg aatgtctctg gaatccattt
    19921 cagaagcttt aacacatagt gatttgagta caacaaagat ttatgttaat acttcaaatg
    19981 ttgttccact tagtgctggc gaaactgctt acagagagct caaaaataaa taaggtaaaa
    20041 ataaggtgaa ttttgaggtg agtttttcaa aaagtgcacg aaaaaacacc caatgaggta
    20101 aacattgagt gtctgtaatc gttgctgtaa agctgttctt aacgttttga gaactggcta
    20161 gctttacgtg ctttcttaag acctggtttt ttacgttcaa ccatacgtgc gtcacgtgta
    20221 agaagtccag cacgtttcaa tgaatcgcgg aaatctgggt caacttctaa aagtgcacgt
    20281 gagataccat gacggatcgc acctgattga cctgcatatc caccacctac aacgttaaca
    20341 aaaacatcgt atgaaccttg agttgaagta actgcgaaag gttggttgat aacaagacga
    20401 aggtctgcgt gtgggatgta ctcttctaca tcttttttat tgattgtaat tttaccagta
    20461 cctgggacca aacgaacgcg tgcaacagcg tttttacggc gaccagtacc tgcatattgt
    20521 gcttgtgcca ttatataatg ctcctttcct cttagataag tcctgagata tcaagtactt
    20581 ctggttgttg tgcagcatgt gtatgctcac cgccaacaaa tactttcaat ttcatacctt
    20641 gtgcacgtcc aagagtgtta tgtggaagca tgcctttaac tgatttttca atcaaacgaa
    20701 cagcattttt agaacgaagt tcacctgctg agatttgttt caaaccacct ggatacattg
    20761 agtgagtgta gtagatttta tctgacgctt ttttaccagt taatttaact ttttcagcat
    20821 tgataacaat cacaaagtca cctgtatcag tgtgtggtgt gaaagttggt ttgttttttc
    20881 cgcgaagtac gctagcaact actgcagaaa gacgtccaag aggtacatct gctgcgtcaa
    20941 caacgtacca tttgcgttca acttggcctg gtttagccat gaaagttgtt ttgttcatga
    21001 tttctcctat acgaattcgt ttttgtttac aggtgtggat gttccggtcc acgagagtat
    21061 ttgaaaggtt ccggggcctt acaaatgggg taaacaatac cgcttataat catatcaaaa
    21121 taaggtagca ttcgtcaagt tatttctttt attttatcaa ttttcatccc aaaataaatc
    21181 atctacagtt ttattcaaag cttgacacaa tttaatcgct taattatagt ctcctttttc
    21241 aatggcggct attgtctgac atgatacacc gaccttttca gctagagact gttataataa
    21301 atccatacta gctctagccg tctttaagcg taaatttttc atattactat ctgtccttat
    21361 acttatggta gtacactgaa attcccatag ctatcaatga tattcctata actgctaaaa
    21421 ttgcatcatc tccccttgaa aaataagtag caatatcaac cttcctagta aaagcatcaa
    21481 taaaatagtt attcctatga gggcaaatgc gccagcaaca aaaacatttt ccaaaaactt
    21541 tcatcaacaa acggagagac gctttttaga ttagaagtcg taacaaaata gaaataccac
    21601 taaataaagt taatattatt ataaaatcag gtaacacact ccccttatta gctttccaaa
    21661 aacactttca agccataaga taaaaaccat cgtctaaaaa catttttgat aactttgtaa
    21721 aaccaattgt tgttgtcgtt tatcatattg aggatagttc ttattttatt ttttataata
    21781 ggtctcctac tattttatag tttcatagca tcatataaaa tacattttat aaaatgtata
    21841 ctaaaagaac ataacgtact gttatgccct ttgatttgtt atcggttttc ttcccctaaa
    21901 gagaatagag ctaaagcacc tgagcccaca tgagctgaaa taactggccc taatggacga
    21961 ataataactt cttcaatacg ctcatctgct aataactgtt ctttcataac ttgagctgaa
    22021 tctttctctc cactataagc aataattaaa gtgctataag atagtgtttt tgttgcctga
    22081 gtcacaatct ctttaatacc ttttttacgg ccccttgtct tggcaaatgg tactaattta
    22141 ccctcactat ctaacttaag taatggttta atcttagcaa cacttccaat gatagctgca
    22201 ccttttgata agcgaccact acgcattaaa tggttgagat cgtcaactaa aaagtatgtg
    22261 tttaatttag ggagaagtga ttcaattttt tgtttcactt cttctaaaga tttaccttct
    22321 tgtcgctctt tagttgctaa catcgctaag acaccctcac cacatgaagc tgccatcgta
    22381 tccacaatct caatttgagc atcaggatat ttgtctaaga ccatctcacg cgcaatagtt
    22441 gcactctggt aagtgcctga taaatgtgaa gatagcgcta aatataacaa ggcatggtca
    22501 ttttctgcat aggttgaaaa aacttcttca aactgaccaa cgttaatttg gcttgttgta
    22561 ggctttgctc cctcttgcat acgctccaac aagaagtcac tagtaatttt ttcgtctccg
    22621 actgtctcat aagtctttcc atcaagttca atggttaaac ctataatatc gacattgtgc
    22681 tcttgagccc acttttcatc taaatccgat gtcgaatccg ttagtatttt aaaagtcatt
    22741 gttacctcaa tctattattt ttatttgata atcaaagtag ttccaatatt atatcaaaaa
    22801 aattgttggc tggctaataa agtgttaggt tttacatacc atctaaaaaa tccttgagtt
    22861 ttcctagttg ttcatcattc cacggcctta actttggcgt actcaaatcg atttgactgg
    22921 acatcttatc caaatctgat tttactgtcg ctacacgctg ttccaattct ctagccgaga
    22981 cacgtaaagc accctgagaa aatatagtcc attgttcatt taaatcactg gtagcaacgg
    23041 agactaaatt taacacagat tgattaagtt ctgccgctgc tcgttcaata tagctatccg
    23101 cagtttcgtc ctcctcagta aagataacgg atattttgta ttggtcataa cgttgacgta
    23161 caccaggaac atactgtgca tcaaatacac aaataatatc aatgtgttcg aaatgtgcgt
    23221 aatgatttag ctttctaagt aatacttccc ttgcctcttc taaacgattg cttttaaaaa
    23281 gctgtcgagt atctttccaa aaagcaatca tattgtagcc atcaacgagt aagatagaat
    23341 gttttttcat aggcggtttc taaatacttc atacatcaaa atagcggctg cgacactggc
    23401 atttaaactc tgaacatgac cattcatggg aattgtaatc atttcatcta cttgcttttt
    23461 gatattatga gaaatacctt ttccttcatt accaataact agggctaatt tacctttggt
    23521 attccactta tgcgatggtg tcccattcat atcagtgcca aatatccaga actccttatc
    23581 ttttaaagta tccaaagttt gactgagatt agttaccctt gcaataggaa catgttcaac
    23641 agctcctgta gatgtctttg aaaccactgg ggtcactcca acagaacgat gtttggggat
    23701 gataatacct gttacattgg ttgcatcagc tgtgcgtaga atagaaccta ggttgtgagg
    23761 gtcggtcaga ccatcaagga ttaaaatgag aggattttct tcattctctg cctttgtcat
    23821 tatctctgat aaatccgcat aggcaaattc cgaaactttt agaacgaatc cttggtgaac
    23881 tccaccattt gtcatgtctg ataaggtttt tttaggagtc caggaaatgg atactttttt
    23941 ttcagtcgct agtgctttga ctttatcaac gttctttcct cttaagtcat cttgcaagta
    24001 taatttatta ccagtattag ctcttaaact ctctgtcaca gcatgtaagc catagacaag
    24061 gtcacttgat tcttctttaa attgtttatc tttcatagtc atattatatc atgaatgcta
    24121 tagcataaag ctttttatag caatatttta ccaacaaatc ctaatgtctc tataaaaaag
    24181 agaggatata gcctctcttt ttgtttattt ttcaatggtt tcaatacacc actgtattag
    24241 agtttccaag cgctttatct gaccagtcat atccaagtac cccataagag cctcaaaacc
    24301 tgtagacata cgataggtaa taatatcagc atttttagct tttgtatgac tattcgcatt
    24361 acgtcctcgt ttataaatta actgctcttc atcagttaaa atattttctt ctaacatggc
    24421 attaattaac aatgcttggg cattagcaga aacatattgg gttgctttcc gatgtaactg
    24481 attaggtttg gtaaaaccct gcataattaa atggcgtcgg atatacaaag aataaactgc
    24541 atctccttca aaagctaaag caataccatt aatcaaacga acatcaatca cgagtccacc
    24601 tcacgccatc cttagtatct aacaatttaa ttccttgttt tgctaattca tctctaatac
    24661 gatcagcagt tgcaaaatca cgattagctc gtgcttcttg acgttgttct attaaagatt
    24721 caatatcagc atcaagaact tcctcttgga atacaatacc aaaaatttct aataattcag
    24781 caaaggtttc cttaactctt gaagtatagt gtcctgaatt aatccatttt gccatttcaa
    24841 agataacagt aataccgttt gctgtattaa aatcatcatc catcgctcct tgaaatgctt
    24901 tcacaaattg ctctaattct tcatcattag cattctcttg gattggtaag ttgaaagtat
    24961 tttttaaata tttaaggtta acttccgcat catgaacggc cttttcagtg aaattcacgg
    25021 gtttacgata ctgttgtgtc gctaagaaaa aacgtattac ttgtccgtca acgctcttca
    25081 acatatcgtg aacagtaata aagttaccaa gagatttaga cattttctcg ttatcaacgt
    25141 taacaaaacc attatgcatc cagtaatttg caaaggtctt acctgtttta gcttcagatt
    25201 gtgcaatctc attcgtgtga tgtggaaatt ctaaatcagc tcctccacca tggatatcaa
    25261 tagtatcacc aaggatctca gttgccataa cagaacattc aatatgccac cctgggcgcc
    25321 ctttccccca aggactttcc catgagactt ccccagattt agctgatttc caaagtgcaa
    25381 aatctaaagg attctcttta atctctcctt caccatcaac acgaccactt gcaccaatct
    25441 ctaaatcttc caaagtttta ttggctaatt ttgcataatg atgtgattta cttactctaa
    25501 aataaacatc tccattagct tcataggcaa attccttatc aactaaaact ttaacaaaat
    25561 caataatctc atccatatag tcaataactc gaggattttt tgtagcaggt ttgacaccta
    25621 atgcagcaac atcttccata aatgctgaaa taaacttatc tgaaaaggat tttgtatcca
    25681 tacctgcttc agctgcacct tttataattt tatcatcaac atcagtaaag ttagaaatat
    25741 aattgacttg gtaaccacaa tactcaaaat aacgacgaat agtatcaaaa gctactacac
    25801 tgcgggcatt cccgatgtgt atatagttat aaactgttgg cccacagacg tacatgttga
    25861 ctttaccttc attgagaggt ataaaatctt gtaaactgcg agtcatagtg tcgtaaattt
    25921 ttatcatggt ttttcctttt tctgtttttg ataagcttta tacaaacgaa caacatacat
    25981 taaaacaaaa gcagcccaaa taattcctaa gagagggtta ccttgtgcaa actttaaata
    26041 gcttaaacaa gctgctgcta gtatacctaa gattgataat atttgagttg ttacataatc
    26101 cttcacaaat acctcctaaa gtttggacga ataataactt tcttctcgat catgttcaat
    26161 acttcggatt tgaagatcat ccttttgacc atggacacgg acaactttag ctggaacacc
    26221 aactactgtt acgtctgctg gcacatctgc aaggacaact gctgcagcac cgactttagc
    26281 attttctcca acctcaattg gaccaatgat ttgagaatgt gctgaaatta gcgctccttt
    26341 acgtatggtt gggtgtcgtt tgcctttgtc tttacctgtg ccacccaatg taaccccatg
    26401 gtatagcata gcacctttct ctacaatggc agtttctcca atgaccaatc ctgaaccgtg
    26461 gtcaataaaa accccttccg aaatcgtcgc tccgggatga atttcaattt gtgtccagaa
    26521 acgccaaaat tgactgtgca tacgtgctaa caatttaaaa ttatgattcc ataaaaaatg
    26581 agatagccta tgagctgcaa gagcttttat tcctggataa gttaaaataa cttctagaga
    26641 actacgagca gctggatctt gttctttgac aatagctata ctttctttcc accaccccat
    26701 agttatcctc aatcgttttc tacatcagac caatcaacta aaacttgttg ttcatcaaat
    26761 ggattgatgg ctactccaaa ggtatcatca atctcatttt ctccattacg cggtttataa
    26821 atatcgtcaa tcgtccaggc taaaattact ccttgtgctt tcctaaaagc accaccaaaa
    26881 tcattatggt tataccattt ttcaaaactt aaaaggtttg agaatactgg aacataactt
    26941 tttccttcgg gggtagacat tgtagggaaa agacggtcaa aactaccttc ttcacgtgga
    27001 tggacaaagg caggtaccaa gtaggtttta tccatgatat cggcaacaat gttatcttca
    27061 tttaacaact gatttaagat tgtggtgtaa ttattcataa attgcaccat atcttcacag
    27121 tagaaaatag tcgagttccc aaaatcgcct tcttttttta aattatagac caatcctgct
    27181 aagccatgtg aaatagcttc atcaagaacg tctaaactac gacgactctc ccaaaacata
    27241 tctcgagcac tttcttgctc ctctttgaaa tgattaaggt cagtaatatc cgtaaaaaca
    27301 ggaacaactt cttgaccatc tacttgaata gcatagggtt ccttgcttgc ccaaaccgta
    27361 gagtggtgca aggcattcac aagacctata ctatctaaaa aattatccgg tgcattgata
    27421 aaagcacgca atctaatgtc taactcgttt gtacttgtca tattaagatt cctttttagg
    27481 attgtcagct tttggaggac gtggtaacaa tgctttcata gaagcatcaa ctcgcccttt
    27541 atcatcaatt ttgatgactt tcacatcaac ttcttcacca atttcaagaa catcagctac
    27601 atttgctgta cgtgtccatg caatttctga aatatgtacc aatgcatctg ttttatcaaa
    27661 gaggttgaca aaggcaccga atttctcgat acgaactact ttagcatggt aaacttctcc
    27721 cacttttgct tcacgtacta aacttgcaat aatgtctttc gtacgatcaa tagcagcctg
    27781 atcactagag aagatagata catttccttc ttcatcaata tcaatcttaa cacctgtttc
    27841 agcgatgatt ttatcgatag tttcgccacc tttaccgatg acaactttaa tcttatcaac
    27901 atcgattttt atcatatcaa tcttaggtgc cgttggtgct aattgtggac gtggctctgc
    27961 gatagctcca tgaaggacat caagaatttc aaagcgtgct tttttagctt gcgcaagagc
    28021 ttcttccaaa atttgagggg taataccttc aattttaata tccatttgaa gtgctgtaat
    28081 cccttcacgt gtacccgcaa ctttaaagtc catatcgcca aaatggtctt ctaatccttg
    28141 aatatcagtg aggactgtat agtttgttcc atctgatatg agccccatgg caataccagc
    28201 aactggagct tttataggca caccacctgc cataagagca agcgttccag cacatataga
    28261 agcctgagat gatgaaccgt ttgattctaa aacttctgca actagtcgga tagcatacgg
    28321 aaactcctcc aagcttggaa ggacttgttc taatgcacgc tctcctaaag caccgtgacc
    28381 aatttcacga cgccctgcag ctccataacg tcccgtctca ccaactgaat attgtgggaa
    28441 gttatagtgg tgcataaagc gtttcttata ctctggtgtt agcccatcaa tgatttgtgc
    28501 ttcacccata ggtgctaacg ttaatactga taatgcttgg gtttgtcctc gtgtaaagag
    28561 acctgatcca tgaacttgtg gtaaaaaatc aatctctgcg tctaatggac gaatctcatc
    28621 cacacgacgt ccatcaggac gaatcttatc ttctgtaatg aggcgacgaa cttctgcatg
    28681 ttccatttgt tctaaaattt cagcaacatc acgcataatg cgatcatatt cttcatgttc
    28741 agcgtaacgt gcctcatatt cacctataac tacttcttta actgcttctg tagctgcttc
    28801 tctagccttt ttctcttcaa cttgtactgc agcctgtaaa gctatgttat gagtagcaat
    28861 aatttctgct tgaagttctg gatcaacttg aagaagttct acctcggctt tttctttacc
    28921 aatagcagta acaatctctt cttgaaaggc gattaattca cagacagcct catgtccttt
    28981 aagaagagcc tctagcatga tttcttctga aagttctttc gctccagatt caaccatgtt
    29041 aatcgcttct ttagtacctg caaccgttaa ttctaacgca gaagcctctt gttcttgtgc
    29101 tgtcggatta ataataaaat taccatcaac ataagcaact tgaactccag caattggccc
    29161 attgaaagga atatcggaaa tcgataatgc taatgatgaa ccaaacatag cagccattgg
    29221 tgcgctagca ttttcatcaa atgaaagaac agtattgatg acctgtactt cgttacgaaa
    29281 tccctcagca aacatcggac ggattggtct atcaataaga cgtgctgtta aggtagcatc
    29341 cgtacttgga cgcccttcac gtttgttaaa gccacccgga aatttcccag cagcatacat
    29401 tttttcttcg taattgactt gaagtgggaa aaaatcacct gttgacattt tcttagacat
    29461 aactgctgct gtgagtacag tagaatctcc ataacgaacg acaactgatc catttgcttg
    29521 tttagccact tggccagttt caacaactaa tttttttcca gcaaaaatca tttcaaagac
    29581 ttgttttgac atattattct ccatattttt ttgtttacgc gtttcctaca aagttcaatc
    29641 tagctgttct aaactcaact ttgcataaaa ccacgtataa gctttattct aacataattc
    29701 tataaaattg agtagcgaaa atccgtggag gaagctgtat tatcattcat taatgtaaac
    29761 gtaaaaagtc tgattattaa tactagcaat cagaccttgt tacaatgatt aaaataaatt
    29821 tctagggtta atcttagctt gcttaataac cctcttcata ggttaaaaca ctttcttact
    29881 gctggcctcc tcaaagtact tacgttcata gtgcgtttaa aacaaaaaac actagaaaat
    29941 tttctagtgt ttttacttga ctctgttgtc tattaacgac gaagtccaag tgattgaatc
    30001 aattcacggt aacggttaac atctgtacga cgtaggtatg ctaataagtt acgacggtga
    30061 ccaatttttt tcatcaatcc acggtaagta gcgtggtctt ttttgtgttg tttgatgtgg
    30121 tcgttaaggt ggttgatttc ccaagtaaga actgctactt gaacttcaac tgatcctgta
    30181 tcaccttcgt gacgtgcata ttgagcaatg atttcatttt ttttctcttt tgagattgcc
    30241 ataatatatt ctccttttat tgcttcatcc gagtcttagg attggcactc ctaaaaccaa
    30301 gaaaaagttg gtttgtctta tagaccctct tattttatca aattagaata aactttgtca
    30361 aaagttttta atagaaatat ggataaccaa ctgtctctaa aggaaaatcc ttcatagact
    30421 aaacgagact ttgcatcttt ttgccgaaag accttagcat aagtagatgt catactagtt
    30481 ggcccacaca taaatattgt tggttgtcct tcaaaaacag actgactaaa atcaagtctt
    30541 cctttgaggc tactattatt taagtgaagt ttaaaattag gattggcttt agcataggat
    30601 tctaacatat cttgataaat caaattgtct tgatttgaaa aggtataaaa gaagtcaact
    30661 ctcttagtca aaatactatt ttctctaata aatgaaatga agggagtgat gccgatacct
    30721 cctgcaatcc aaacctgttc tttcttgtct ttatcaaata acatatgacc ataagctcta
    30781 tctaaggcta tttttgtccc aacttttagc tgtttgtaaa tggatttcgt ataatctcct
    30841 gaagctttaa cagttaaaaa tatcactcta tcatgaccac ctgatatcga gaaaggatga
    30901 gctgctgatt caaagccagc ttgataaatt ttaaaaaaag taaattgtcc atagtcatat
    30961 cgataaggac gcttcattgc tatttcaatc tcagttgtat catggttaag atgagtaacc
    31021 ttttgaacgt accctacacg acgaaaacgc attctagaat ataggaaaat aatataaaat
    31081 ccagatataa ccccaatgac agcataacct aatacgatta aactgagtaa agtatttcct
    31141 agtatcctat ctcctaaaat cataaaagta tgtaccaatc ccaaaatata agctaaataa
    31201 acaaagcgat gaataaaacg ccaaatttca tactttaaaa aatttccgaa ataagctaca
    31261 aaaacaatac ttagaaaaag atataatccc gcactgccaa tagtctttgc aaactcactt
    31321 ccatgtccgc cttgtccaag accaattttg tgcaatagaa gcaaaatcat tgaaaataca
    31381 gccattacct tgtggtaaaa atacatattt tcaatacctt gaaagtaact ttcaagtatc
    31441 ctaaatttgg ttgataagag gaatgttagc gataaacttg tcaaagctaa tcctggtaca
    31501 acaaattcac ttaatcctgc attttgccat gctactagtg tcaccactat gctagataaa
    31561 attaaaacaa tacctttcca agctcgcata acgaccaccc ctttattttt agtaaggata
    31621 aaataccaca agatacttat acaaaactta aaaatttcag aaaataacaa aaaggcaact
    31681 ccaatgttgc ctttttgtta tgatttataa attgatttgc aatgctgtac tatatcttta
    31741 acagcgagtc catactcctc taataaatat tccatctgac ctacctgacc aaatcgctca
    31801 tcaataccca tacgactaac agtagtgtct ttctccatac taagccattc gcataatgca
    31861 cttccgatac cgccaatacg gttatgattc tcaatagtta caatggattg atctatcagt
    31921 aatggtttca gttcttctgg aaggggctta attttaaaaa ggtcgataac tgaggcctca
    31981 atccctagtt ctttgaggta gtcggctgct tctatcgctc tagaaaccat aataccactt
    32041 gctacaagag taatatcttt tccctggcgt aattgtataa aaccctttga aaaatcttcg
    32101 cgtccctcat aaactgcagt gggggcctta cgaatagttc taatgtattt taggccatca
    32161 atacttaatg tttgttttaa aattgcttca aactgaatat catcactaac ctcaaaaata
    32221 gtcgctttgg gaattaacct tagtagacct aactcttcaa aaggcatatg agttccgcca
    32281 ttcatttctg cgctaattcc tgcatctgag ccaataatcg tagctgataa ttgtgagtaa
    32341 cctaacgata agaatacttg atcaaagaca cgccttgacg cgaaaggacc aaaagtatga
    32401 aggtatggct tgtaaccttt aatggcgagc cctgctgcaa ggcctaccat ctcagcctcc
    32461 ataataccta gattaatata acgttttcca aattctgatg ccagtgcatt tgttgacatt
    32521 gaactcgaca aatcggcttc taaaacggta atttgtttat tttcttgatt agcttgaagg
    32581 aggaaatcac gatagacaag cctcatttct ttggttgaac atctcattct accacctcca
    32641 aactttctct caagcttatc aacgctcgct ctaacattgt tttttgttgt aaatctggtc
    32701 gtaaatgatg attagaagct aactcctcca actctttcac accttgtccc ttgatgctat
    32761 ctaaaacaat acattttggt ctgacactat ttgaatcttg aaaagtttta atagctttgt
    32821 caattgcctc aatgtcatct ccttttacac gtactgcatc aaatccaaaa gcttcaaatt
    32881 tagcaacaaa gtctccagga ttacaaatat cagctgtcaa accatctaat tgttttttat
    32941 tatcatctac aaaaacaatt aaatggtgca gttgatgatg cgcagcaaat tgtatagcct
    33001 cccaacattg tccttcattt aattcaccat cacctacaat agtataagta taatagcttg
    33061 aattctcaat cttttgagca taagcaattc ccgttgcaat actaatgcct tgacctaacg
    33121 agcctgtcgt tacatctata ccaggagtta aattgcggtc aggatgcgaa ggtagtttgg
    33181 taccgtttgt attgagcgaa tgaaggaatg ttttatcaaa aaaacctttt aaatagagtg
    33241 tactatacaa ggctggacca gcatgtcctt ttgataagac catataatcc cgatcacttt
    33301 ctttgaattt ttcaggattt atatccataa tatcaccata cagaacagcc aaagcctcaa
    33361 caattgataa actaccccca tagtgaccga atcctaaatg gtttagcgtt tctagagtat
    33421 ttaaacgaat ctccgttgca aatcgtctta gagatgaata tctttcttct ggtgatgtca
    33481 ttattaacct tccttatggg ttgattttcc aacaaatgaa atgccaaaaa gtccaattaa
    33541 gataactaca ataccaataa caatagctat cgcaccgccg acatgattaa gagcgccaag
    33601 aatgataccg gtcaaaccaa aatcagcatc tgagaatgtt gaccctttga aaccaagtcc
    33661 acctagtaca ggcatcaaga aaatcggcaa gaaactgatg aggatacctt gaacaaatgc
    33721 accaatagtc gctcctctca caccaccaga agcattaccg ataacacctg ccgttgcccc
    33781 acagaagaaa tgaggaacga cccctggtaa aatgacagta gtgccagtca caatcatgac
    33841 tatcatacta accaatccac caacaaaact tgagataaaa ccaatcaata ctgcattagg
    33901 tgcataaggg taaacaattg gacaatccaa ggctggtttt gaatttggaa ccaatttttc
    33961 tgagattccc ttaaaggctg gtacaatttc acctaggata aggcgtacac ctgctaaaat
    34021 aacgaataca cctgccgcaa attgcccagc tagttggagg gcataaacaa gaccattgac
    34081 accgttactt atttcttttg caatataagc ctcaccagca aacactgcaa cgataaggta
    34141 aataattgcc attgagatag taatacttac ggtactgtca cgtaaaaatg ataaactctt
    34201 tggaaattta atatcttctg ttgattttga tttatctcca acaattccac caataaatcc
    34261 actcaaccaa tagcctaaag aaccaaagtg cccaagtgcc accttatcat tacctgtcag
    34321 ttgaatcatg tatttttgca caaatgcagg agatacgctc ataataatcc ctaacgctag
    34381 acctccaaaa aggatgagcg agaacgaagt aaagcctgct acggcaaaaa taacagcaat
    34441 catacaagcc atatacaatg tatgatgtcc tgtcagaaaa atatatttaa attttgtaaa
    34501 acgagcaatt aaaatattaa aaatcattcc cgctaacata atcaaagcag ttgctgagcc
    34561 atacttcgta agagctactg ctacaatggc ttcattatta ggaactaccc ccaccaaatg
    34621 aaaagcatgt tcaaacattt ttccaaatgg attcaaggaa ttttggacta tccctgtacc
    34681 tccagaaacc actaagaagc caacaaatgt ttttattcca ccttttacaa tatcaggaac
    34741 gccttttttc tgcagtactg aaccgataat ggctatcaag gcgactaaaa tagctggcgt
    34801 gctggcaata ttaactaaaa aatccaatag acctttcatg gcatcaacct cctagattaa
    34861 gcctttttct tggcaaatac ctgtcactaa ttctctcaat tcatccatat caatgatact
    34921 gtttaaaata cgaacatccc ccaggtgacc agctgaatcc tccaaatcac gaccaacaat
    34981 ccaaacatct gctgcacttg gatctgctcc acctaaatca taatgttcaa cttcaacatc
    35041 agaaaccccc aaatccttca aaatagactc aatattcatt tgtaccataa aacttgaacc
    35101 taaacctgat ccacatgctg ttccaattct taacatcttt aatcctcctg ttttataatc
    35161 atttcaatat cttgatacct acgtgaagct aacaaagctt taacattatt attatcacga
    35221 agtattgttg tcaaatgcga tagagctttc aaatgcgttt cattatcaat agctgctatg
    35281 cataataaga gataaatttc ctgtttgggg tcatccaata aataaatagg ctgttccaaa
    35341 acaagcattg acataccgac agagtttacc ccatcttctg gtctggcatg tggaatagca
    35401 atacctttac caagattgat aaaaggacca aactcttcga ctttttcaat catagcttct
    35461 gggtaacttt ccgtaatttt tccagaagct aacaagggtt ttgccgctaa acgaattgcc
    35521 tctttccaat tcatttgttc agaacttgtc tgataagttt cctctgttat aagttggtgc
    35581 aacaagggcc tcacatcctt tctaagtata tcttgtttta ccattgtcct caaagccaat
    35641 tttaattcct cttcacaatg aacatgagcg tattttttta tagttgctat taattgatca
    35701 agttggaaat cgtcaaggca agccttggga aaatctgaaa taaccagttc tttgagctgt
    35761 tgtacttgct ctgctgtcat catcagcgaa actagataat taggtttttt tgtttctaca
    35821 aatatagtag aaaagaccat atcgtaagtt tgattgtcaa tcagtttcag ctgctcaatt
    35881 ttagagacac gatgaaaatg aatttgagga aataatccac gcaatttttc cttaatcact
    35941 aaggatgaac taacgccgtt tggacagagt atcaaagctt tataagacat tgattgtgta
    36001 ccacctgatt gtctaagata gcctccaaag tgaatcacga agtaagaaat ttcactatcg
    36061 ggtattaatc ctacttgctc ttccaaagga cgtagtgcct ttttaaccaa tagaaataaa
    36121 tcactgtaat gctctttaat attttgtgta taaccactat cgccagttaa accatatttc
    36181 aatcggaaat aagctggaat aatatgacgc ttcagacctt gtaataactc ttctttattg
    36241 gaaaaattaa gaagtgaaac agtttccatc tcatcaacga ttgcttttgc caatgcttca
    36301 aagaaatcat catctttagt tccctcacct tcaaaacagc cagataagat caaggtaata
    36361 taatcttttt ctttctgatc taatggttta gataaggaaa tatcctgact taaattagtc
    36421 acaataatag tcgttaactc ttttaattgt tcagaaacaa taggagaacc ctgcaaaaca
    36481 cgatctactg acctttgata acgacacagt attaaaatga taaaatataa agatttctca
    36541 agcctatctt gtattggaga aagttgaaaa gattcataga aatattctac cgtcttttct
    36601 aacttctcat atgagagagc gaatttccaa gaagacaaga tataattcaa agaccaaaaa
    36661 ccaatgggag attctagtag ttgagttatc atctgaaaag caacttgatg cttatccatc
    36721 tcatctccta ctagactata tccctttgct cttgtataag tgagtgagat acctcttttt
    36781 gctaatttag aacgtagcat cttgatatct gataaagtag tattcttact tactttcaaa
    36841 aaatcttgat aatgcacatt ggagacaaac tctcttcggc aaaagctata aaggtaaatc
    36901 agacataccc tttcttcctg ggttaggtaa aattgctcag cttctaataa ggagacaatt
    36961 ttatcatgct ctttttcatc tcctaataac tgataacgtc cagcagagtg agcaatagct
    37021 ggtaggtgat tttttgctaa aaaactattg agatagttaa tttgctctat gataactttt
    37081 attgaaaact ttgtttgcac tgttaactca tacaaggaaa gatttttccg agctaaaaat
    37141 accaacatta tcacaacagt cttattatca accatttgtt ttcctttcta tttttatttt
    37201 atagtaaccg tttacatttt tctaggtcat ttgagcccaa tctggcacta ctatcaattg
    37261 aatgaatttg ggctattgtt atagagtaaa gaaaaaagaa agaaattgca aaatctagag
    37321 caagattcct ttccacaagc agcactttaa aaacctttac ttaattgaat aagtaaaggt
    37381 ttttaaaatg ttatgacact aagatagcta aagtttgata aggttgcaaa gtaaccgttt
    37441 catcaagagt aacatcctta taatttgata gaagaacttg tccttggaga taattctccg
    37501 gtaattttat cttaactttc tcagcaaaga aattatttaa aacaagaagt ttttccttat
    37561 caagatggcg ttcaaaagca tatacttttt cattatcttt gaaagctgct ttataattcc
    37621 cgtcagctat tattggcagt tgtttacgta gccttatcaa ctcttgatag aaagtaaata
    37681 tcagccctgt tttctccttt gcgacattga tttctttata tgattttcct actttcaacc
    37741 atggagcgcc ttcagaaaaa cctgcattcg tactatcatc ccattgcatt ggtactcgag
    37801 aattgtctcg agatttcgct cgaataatgc taaatgcctc ttcttgtgac ttgccttcat
    37861 caagcattat ttggtaggca ttcaaactct caatatccac ataatcatcc atagaactgt
    37921 aatcgggatc aagcattccg atttcttcac ccatatatat ataaggagta ccacgcgata
    37981 agtggataga tgctgctaac atggttgctc cttcatttcg aaaacgtttc acgtctacaa
    38041 aacgatttaa tgctcgcggt tggtcatgat tattatagaa taaagcattc caaccatttc
    38101 cttcgctcat cccttcgccc caactatgga aaaggtcacg taatgcagga aaatcaaaag
    38161 ccataatggt ccacttttgc ccatctttat aatctacttt gaggtggtga aaattaaaag
    38221 ccatggataa ctcttcacgt tctggtgcag tgtaaagaat gcaatttgca attgtcgttg
    38281 aagacatttc accaactgtc atgaaggaat catcttgtcc aaaactagca ttattcaaca
    38341 tcttgagata atcatgtgtt attggacgat cagtataagc tggttttcca tcattaattg
    38401 ggcagttctc aagtatctca tctttcccaa ttaaattaat cacatcaaat cgaaaacctt
    38461 taaccccttt atcacgccaa aaattgacaa ctttaaaaag ttccttgcga acatcggcat
    38521 tgcgccaatt aaggtcagct tgagtgatat caaaaagatg aagataatac ttccctgtat
    38581 caccaaatgg tgcccaagca tttccaccaa atttagatac ccaatctgtt ggattatcac
    38641 gtaagataaa gaaatcttga tagtagcgat ctcctgcaag tgctttttta aaccactcat
    38701 gttctattga acaatgatta agcaccatat caagcataaa atctatacgg tattgacgtc
    38761 ccacctcaat catttcttca aaatcgtcca tcgttccaaa atcagggtta atagctgtat
    38821 aatcagagat atcatatcca ttatctcgtt gtggacttgg ataaaatggg ttgagccaaa
    38881 ccatatcaat accaagttct gctaaatagg gtaatttttc gataatacca cgaagatcac
    38941 caaccccatt accagttgtg tctttgtatg attttggata aatttgatag acaactttac
    39001 gtttatcaat cgtcatattc tactcctttc ccaaaatgct gctgtattta gcagtctaac
    39061 tataacatca actatcatca aaaatataac atttttacag tgatatttgt tcctatgagt
    39121 tttttcttat gttcaaaata attcaaggag ctaatgtatt aagcccctca aacaattcct
    39181 caaattacct ctcattttat aagagtgctc ttttttaaaa agaataccta attactttta
    39241 aattttcttg gcaaccatta atgcttgtcc acgttttatc tgtttgggta aatctatcaa
    39301 ttcttctggt cggaattctt gttgattagt aatcagaata ggagtttcca caatgtatcc
    39361 ttcttctttt atcattggaa tatcgaaagt aatgagttta tctcctactt ttacagtgtc
    39421 accttgagca acatgagagg taaacccttt tccttctaaa ttaacagtat ccataccaat
    39481 atgaataaga aactcaaccc cctctgatgt taacaatcca atagcatgtt tcgtagggaa
    39541 aagaactgaa actgtcgcat cgaccggtga aactagttct ccatcacttg gatcgataac
    39601 cactcccttc cccataattc cttgtgaaaa gacaggatca ctagcttgat ccagtggttt
    39661 agctaaacct gataaagggc ttactacact aagctttgta cctgatacga cagctttttc
    39721 ttttgctgat tttgtttctg ttgttgaagc aatgacagca tcaggtacta atttttcctc
    39781 ttcagttttt gtcaaaatac ctgatttttt aaagaatagt gtcaaaaata aaggaataaa
    39841 gatagccact gccatacaga taaagaaata tcccatatat ttaacgttaa ttgacaggaa
    39901 gcctggaaga ccgcctacac caatagagtt agcttggaca ttaaaagttg tcgccaaaag
    39961 acctgccaca cttgaaccaa tcataccagc aacaaatgga taaatgtatt taacgtttac
    40021 accaaaaagc gccggctctg taacaccaag gtaggctgag atagcagctg gaagtgatat
    40081 ttgagcctct ttttcatcat gacgatgcat aaaataataa gctagtacag ctgaaccctg
    40141 agcaatattt gaaagagcaa tcatcggcca aaggccagtt gtatgtgttt ttgtatctgc
    40201 tatgagttga gtatcgatag catttgtcat gtggtgcaaa ccagtaataa caaatggggc
    40261 atacagggca ccaaaaatag caccaaagag ccattttaca ggaccagtca atccaataag
    40321 aacgatagca gaaatccatt tacctagtgt ccaaccaata ggtccaagga cagtatgagc
    40381 caagataata gctggaacta aggatagaaa tggcacaaaa atcattgaaa caacttcagg
    40441 aatatgtttt ctccagaaga tttctaagta agacaggctc aaaccagcta acagagctgg
    40501 aataacttga gcttgataac caattttttg tactgtaaag taaccaaaat tccaagacca
    40561 atttttagca atgtctgctg ctgaagtact agctacagaa taagcattaa gtaattgtgg
    40621 cgacactaga cagataccta gaacaatacc gaggatttga gtagtcccca tcttacgagt
    40681 cacagaccat acaataccaa ctggtaagaa atgaaatata gcttcacctg gtaaccataa
    40741 aaaagagtct actccgctcc aaaacgttga aacatctact agagtattcc aaattggatg
    40801 cccagaagag tctacttgcc tgacaccatc aacaactttt tgacctaaaa attcaaatgg
    40861 aacggcatct aaaatattac gaaaaccaag gatcagacca ccaacaataa tggctggaat
    40921 aatcggtgta aatatttcag ccagcattgt caacacacgc tgtaaagggt tttgattttt
    40981 ttgggcagcc gattttgccg cctctttaga cacaccctca ataccagaaa cggctacaaa
    41041 agcattataa aaaataggga catcattacc aataatgact tgaaattgcc cagcattggt
    41101 aaaggtaccc ttgactgatg gtaactcctc aattaccttt accttagctt tactactgtc
    41161 attcaaaaca aatcgcatgc gtgtagcaca atgtgtcact gcactaatat tttctttacc
    41221 cccaattgcc tctaacaagg ctttggcatc atgtttaaat tgttccatct gtttatcctt
    41281 atctatttaa cggagagtct gtgactccaa ggcagaaact taaataagac tagcttacaa
    41341 cataattgta aacgattaca tatccctatg caaatcctta gcttatcttt ttaaaaaatc
    41401 tataaattat aaaataaatt agtcaataat acccaaaaca ttagaatttc tcaccaacct
    41461 taccaaaatg ttagagtcat gagtagatca gtcaccctca atactctctt aataaaaaac
    41521 acctactaat tgatatagta ggtgagtttt ttaatctcca ttgaaagata cgatacgatt
    41581 ctcttcaatc atatagcatt ggttacaaag tttctctaac atagcgaaat catgactaat
    41641 aacgatgaga cctaaatttc tatcctttac aatctccaac aggcttttcc atacactagc
    41701 ttgtgtaatg ctatccaaca tagtagtcat ttcatctgca ataaggtatt tagtctctgg
    41761 atgtaatgaa cgcacaatcg aaaagcgttg taattctcct cccgagagtt cactaggtcg
    41821 acgatttagc catttttctt gaattccaaa agcatctagt aaatctcgac ttgggtaata
    41881 ggcttcttcc aaactttttt tcataggcca taaaggattc atcgtttttt caggatgttg
    41941 ttgaataagt tgtacaggtc taaatgcttt gctaggcaaa ttactgccat caactaatac
    42001 ttcaccagat ttaggatgta aaaaacctgc aagcaccctg gataaactag tttttccaca
    42061 accactttgt ccaaatatac ctaaaacttg accaggtgct acctctaaat taatctcctt
    42121 aaaaagccat tgatcttttt tatgataaaa gccaagcttt ttagcctcta aggtcatgag
    42181 taactccttt caaaaattct tgctgtggga gagagcgcca taaacttcta gcaaattctg
    42241 tttgtaactg ctctccattt ccgctaaaga aactagctgg agctgtttca atagcttttc
    42301 cctctttaaa aatagtaata cgatcagcaa tttgactagc tgctacaata tcatgagtga
    42361 taaatataac gcttattcct ttatctgcaa aggagcgtag ttggtctaaa accatttgca
    42421 gagcatctgg atgtaatcca ggggtgggct catccgcaat aatcaaagaa accttatcac
    42481 taatacacgt tgtaaacaaa acacgtcgga gcattccgcc agaaagttgg aaaggataca
    42541 agtcaccatc actttctttt aaaccaaact gttgaaacaa tccttcttga gtagcctttg
    42601 aattttctga gatacctaag cgcacctgat gtttgacttt cgtagatgga tctaaataat
    42661 taactgattg tggaatcaac gtaatatctt ttcctcgcaa ctgtttaatg cgtttagaat
    42721 ttagtgattg accacgataa atcatatctc ctgttacaga tgcattttta ggaagaatat
    42781 ccataatagc atgtgctaat aaactcttcc ctgaaccact agcacctata atagctaata
    42841 actcaccttt tttaatttct aaattcagcg cttgtatcgg tgttgattga aatggtttta
    42901 aaaatcttcc gtattgagtg aaggtgatgg agaggtcttt aatgcttaat aatgtttctg
    42961 tcatatctct acctcctaaa aatgatcagt ttgagggtaa aagagtttct ttaaagattc
    43021 tccgatagta tcaaatgcat tgacaaccaa aataagataa agtcctggaa agataaccaa
    43081 ccaccaattt ccaagagaga tatgcttagc tgcctctgac agaatgatac caaccgaagg
    43141 ttgttcggca gagagcccaa atcctaagaa agtcattgat gcttcatgta ggatgacatg
    43201 tggaaataag aggataaaac caatgaaaat ttgagaagca atcaaaggca ggatatgatg
    43261 cctcacaata taataaggcg tttttcccat acttttagaa agttggacaa attctttatt
    43321 ctttagatga tagacttcat tgcggataag ccttgctaaa gaaggccaat gtgtaacagc
    43381 cgttgcaatg atgacccctt gagcaccttt cccaacaaca aaagaaatga gaatcataaa
    43441 aatcaaatga ggcataccaa taaacaaatc aactaaccat gctattattt tatcaataat
    43501 gctatttcct aaacctgcaa gcactccaaa aactgtcgcc agaatgaccc ccataagggc
    43561 acctaataag ccgacttgta gagagaaata aagtccttta atcgttctga caaacatatc
    43621 cctacctaaa ccatctgtcc caaaaagatg gtttaatgaa ggagcaaggt tccgtaaagc
    43681 tgcattggtt tgcaagggag tactatggaa ataaagattt aaggctagaa tactaagaat
    43741 gagagaaatg gcgataccca gttgccataa aacaatagtt cgacgtctca atatcatact
    43801 tttctcctta actgtggatt gattatgctg tttaaaatat ccgcaataag attgcccgca
    43861 aaaacaaata atgtccctat catcacaata gctagaagta gcggtgtatc acttttaagt
    43921 cctgcttcag ttagggtaga ccctagtcct ggatatgaga aaacttgctc agcaagaacg
    43981 gatcctccaa acaattctcc aaaataggaa aaatgcagtg taatagctgg tacgatagca
    44041 tttctaagac aatgattttt aaaaatttgc cattccgttt ccccacgcgc tctggcaaat
    44101 aagacatatt cactagaaag taccgacatc attttagttc tagtatgaag agttacattg
    44161 gcaatgccta gaatacttag cgtgaaaaca ggtaaaataa ggtgcttaat acgatcagct
    44221 aacgtaatat cttgactcaa agtacctatc ggggaagaaa taccaatcgg gaaccacccc
    44281 agctggacag aaaagattaa taaaaaaatg agcccaatcc aaaaagttgg tactgatatc
    44341 tgaaggtaag aaaaccacct tacaattcga tcaagcaatt tcccttgatg gaaagctgat
    44401 aaagttccta agataaatcc aataagaccc gataagaccc aggagagtcc cataagtatg
    44461 aaagaggcac ctgcccgtga tctaataata tcaataacag gttgccgata aactagtgaa
    44521 gtccctaaat gtccctgtat aaaatttttt aaccaaataa aatactggac cggagctggc
    44581 ttatccaaac catagtggtg agcaatcgct ttgtactgaa caggggttag tgacgtgtca
    44641 taattgacac ttgccatgac tggatcaacc ggtgattgtt ttagtaaaat aaaggtcaaa
    44701 attgatactc caaaaacgag tgacaaacac ctaagtatct tccagataac tacagtggta
    44761 atatgtttca cagataaatc tccttaattg ttaaaaaagg agtcgaggaa accattcaca
    44821 ccataaggtg tacattgttt tctctgactc caaacttaaa gtagcctaca ttaactaaat
    44881 tagttactta gcagattcat cccaagtcca ttcagcaatg ttagtcaata atgaccaatc
    44941 atgaccatga ctatggacac cttgtttacc tacattgata cgtttatcac caatataagt
    45001 atggttaaga ctcaccaacc atacatttgg taaatctcca agagtggaag cacctgtttt
    45061 gccatcccac tgagctaact tccaatattt gttagcttta tcaaggtcag gagatgtcat
    45121 tgctttgtca aggtacttag tcacagtagg attgttataa aaagtaatat tggtccaacc
    45181 tttaccagct aaacttgggt aatgtgattc ataaaattgc tgcgcatgat gacgtcctcc
    45241 cgcataaagt aaggctgaat catgtgactt agttgccatt tcatcccagt tactagcctt
    45301 gagtttaata gtaataccta aggctttagc ttgctccgct acttcaacgg ctaagtttgc
    45361 tcgtaattga tcattagtag ggtagtaaag gtcaaattcc gctttaagat ttcctttttt
    45421 acggctaccg tctgcttgtt ctttccatcc agcttttgtc aaaagttgct tagctttagc
    45481 tactttatta tctttaattg ctgtttttgg attccagaat ggtgttctat caataattga
    45541 ataagctggt ttaccataac catttaaaac agtatccaga actttttgtc tatttaaacc
    45601 aatagtcaag gcttttctga ttgctggatc actagtgaca tcatttccta ctggataacc
    45661 gtctggtgaa tttttcacaa cacctttctt cacataaggc aatgacagtc cgcgaacgtc
    45721 gtttgaagca atatctagaa gacgtgttcc tttgactttc ttgctagcaa gctctggcgt
    45781 tgcgtaaatc atgtcaacat caccagattc taaagcagct agtgctgtat tttcatcaag
    45841 taatacccaa gtccactttt taaaataagg ctttttaccg tgccaatagg gatttcgaac
    45901 gaagattgct tgttctccag ccttatattc tttcaccatg taaggtcctg aaccgatagg
    45961 attgctctta tacttatcat tgtaatgttt tttagggacg attgggattt ctgtcaactg
    46021 tgctgtaaat gtcgaatgcg cctcagtcaa atgaatattt acctggtttt tcccaactac
    46081 ttcaacattc ttaataaagg tcaaatccca agcttttcca tctgctttca acatatcata
    46141 agtaaactta acatcgtcag cagtaacagg ctcaccattg gagaatttaa aatcatcatt
    46201 taagtcgaac gaccacgtta agccatcctt agagatttta tattttttag caagctctcc
    46261 ttttatatct agttcaggag aacgtttcaa tagggtgcta tgagtaatat taccttcatt
    46321 atggattcca taacggtcct ttggatcgaa ttcatgagga agctttgccc ccatagaaac
    46381 aaccaattca tctttgggtc gttgttttcg cgttcgctcc ttggtttgtg aattttgatt
    46441 ttgacaagct actaaaaaga tactcgccag aaacaaagca acaaaagaga tgtacttcaa
    46501 atatttagtc actattaaac ctcctttttt aattgataaa taatattttt gacaactttt
    46561 ttcattataa caagaaaaaa gtaggagaac aattacttaa ctggttaatt agaaaatttg
    46621 ttattttatc attaataact taaagttagg taaaacacaa ttctagacac tagctcataa
    46681 accatgttaa tccaatgtat tttaatagac atcataaaaa ggctgaataa attcagcctt
    46741 aaccacgcta ttagttatag caaaccaatc aaatgactaa agataggaat aaccaaatca
    46801 actaccaaac cgataaccac cacagctata gctgccatcg caccttgcag ttctcccaat
    46861 tccaatgcaa aggctgctcc gactgtatga ccagaggtcc ctagacctaa tcctgcacca
    46921 atcttactat cattcaaatg gaaaaatgac actaattttt tacctaatgc ataaataata
    46981 actgcattta agatacacgc cattgctgtc acagctgtgt tccctccaat tgctgctgtt
    47041 attggaagag caattgctgt tgttgctgct tgtggcaaca ttgaagcaat tccgacttga
    47101 ctaagcccaa ccattttaga aatagcgacg ataagtataa gggaaacaat cataccgatt
    47161 actaaactgc tgagaatttc tacccaatat tttttaacaa cgtcattttt cttatagaga
    47221 ggaactgcaa aagcaattgt cgctggattt aaaaaccaaa atattaaatc accacctggt
    47281 ttataagctt gtgtataaaa tgtcttaaca tcggttccta aaccttttga cataagccaa
    47341 agaatgacaa taccactaac cattgcaaag aaaagaggct gcaaaaggaa gaaaccttta
    47401 ctcttcttaa ataaatgttc tcctatcgta tagagtatta aagaaaagca aataccaaag
    47461 atgggtgttt ttaagagttc catattattt ttcctctact ttcttactat agtggttagg
    47521 taatatcttt ttaaataaat ctgtgacact gagttctctg atagcaagta ttaatcgtgc
    47581 aacatatgct accactacca acataacaac agttgagatt gtaataacag caactagttg
    47641 caagccttca gcttttagga tatcaagatt cgctgctact gatattcctg aggggacaaa
    47701 cataaaacca atcatgctta tcatcaaagc cccaaaagag tcaacccact ctaccttgat
    47761 aattttagca gttagcaata catacattag caccaaacca ataaccgtcg ttgggattgg
    47821 tagtgatttc ggtaataaca ttgaaatcat ttgtgaaact aatagaatag ctgcatatat
    47881 tgacatttga ataaacattg gagcttcttt tgttttggtt tctttactgg tgtgtttcat
    47941 agtcactacc ccttcctttt attacaatgt tttaacttgt tttattgata gctatattgt
    48001 acttcaaaac aaataaaacg ttttcatttt tagtctcagt taccaaaaaa agaagccgaa
    48061 ttgcaaattc aagcttctaa acaacaaagg gaagttcgca aaagcacttt tacatggtta
    48121 aatgctcatt taggtctttt aaatacgatc gacctactgg aaatttctct ccattagaca
    48181 taattagtaa caaagtatga ttaaaccaag gttgaatttc ctcaatcatc tctagattaa
    48241 tcactgtgtt tcgatgaatc tggagaaaat atggtgctac agcgcgtgac ttaaaccaat
    48301 tgagtgtctt ccgaatagtg taagttttct gaactgtact aacagttaat tcgccatctt
    48361 caacactagc tgcaacaata tcttgcattt taacaacaac actgcgatct gataatgtca
    48421 aggttaacaa ttcaacactg gcttttttag gtattgcttg ctctacatct gaagcagtgg
    48481 tgagttggct caatttcttc acttttgata gagccatatc aacgcgctgt tgttcaaacg
    48541 gtttcatgat ataatcaaca gcgttacttt caaaagcttt cacagcgtag ttatcataag
    48601 ctgtcgcaaa aacaacaagt gggggatgtg caagttggct caactgatta gccaaagtaa
    48661 agccattttc ttcactaagc gaaatatcta aaaaaattaa atcaatttgc tgtctaaata
    48721 agattttttc ggcttcactg atatcctcag cctgaaaaat ctcagggttg tcaacctcct
    48781 ggctatgttc aactaaaaac gataattctt gtctggcaaa catttcatca tcaagaatta
    48841 aaattttcat gctcatcctc ccttattcta ttaggtattc gataccaaac ttttgtacca
    48901 ttcttgtcgc tcgaaaaatg aaggcaactt acactaccat ataataaatt cagcctgtta
    48961 tttagattaa ctagagcagt acctgtaccc ttactctctg caactgtttc ttgacctaat
    49021 ttatcaatga tagtatctga gattccttgt ccattgtcac taacagaaac acaataataa
    49081 tgaccatctg gctttatttg aaccaatata tggttgtccg tcttacgttc tttgaaagca
    49141 tgtcgaactg cattctctac cagtacctgt aaaccaaaag gtggtaactt cattttttct
    49201 ggtgcactaa tatcataaga taactgatat ttatcaggga aacgtaattt ttcaacattc
    49261 atataagcat ccacatgtga tttttcttgc tcaagcgtta cctcacgatc ctgaccaccc
    49321 tgcaaacttg ttctaaaaaa agtacttaac tgcatcagtg cataacgtgc tttatcagaa
    49381 tcaatacgga ttaatgcact aattgtgtta atggcattaa agaagaaatg agggttgatt
    49441 tgtgcttgta aagcctttat ctctgccata ctggctaact tattttgttc ctctgttatc
    49501 cccattgcca gttgtcctga aaatatttgc gctaaaccaa ggactaggtt ttcctccacc
    49561 tcagacattg tcttatctcc tgcaaagtac atttttaagg cacccacagt tttatcattt
    49621 atttttagag gaactacaat agcagaattt aactgacagt tgtgatctgg acaagaaatc
    49681 gccgctttat cttgcgcaat tcttggttcg ccatcaaaaa taacactttt agataagtct
    49741 gttttgaccg gttgtcctgc aatatggtga tcatggccaa caccaatatg agctaatacg
    49801 tttgaccgat ctgttaatcc cacagcatca aagttagtat gcctctttat aatttcgcaa
    49861 acgctcctag cagattgcgg tgtcaaacct tgtctaaggt agggcagagt ctgtcgagtc
    49921 aattcaagaa catctctcgt ttgaactgcg cgtaactgac tttcatttga caaataagtt
    49981 ttcaaaatcg caaggaaaag tgtggaacct aaactattta aaatcatcat tggaatgaca
    50041 atcattttga caagttccca tcctgtaaaa atgccaacaa atagcatctg gatactttcg
    50101 gcaataatac taattaaaat aacttggctt gttgaagggt agagatggtt ttccttaagc
    50161 ttatcaccaa tctttccgct aacaatgccg actagaactg aactgacaat atagaaagaa
    50221 cctgaaaagc ttccttgaaa aaagcgatga actcctccaa taaaaccaac aattgatcca
    50281 accagaggtc caccaaccaa acttgccgtt gtaataacta aagtccttgt attagcaagt
    50341 gagtcagaat gagaaatcgt tgttagaaaa gggcgctcga ccaaacttcg atcccctttt
    50401 atttcaattc ctgttatatt agatataata acaaacaagc cgaaaatgat gacaaggact
    50461 accgtttcac gtttagaccg ctcttcaatt aactgtctaa aataactatt atttaccaat
    50521 aaaaaggcta aaatcataat aattcctagc ctttggaata acaacaccat caacatatgc
    50581 ttatcctcct caagatacca ttttactccg gaagataaat atagatatct tgataatctg
    50641 aaataacctt gtctgccatt gataaatctt gcggtggata atcaggatta gcaaagccaa
    50701 agcaatacat tcctgctgcc ttagcagcta aactcccatt tcttgtatct tctataacta
    50761 tacaaacttt agggtctaca tctaaaagct ctgcagctct aagaaaaaca tctggtgctg
    50821 gtttagaaga cgatacatct tctcctgtaa ccatgtattc aaagcattct gtcacaccca
    50881 actcttttag attacgctta atatctacca ttggagatga ggacgcaact gctaagcgat
    50941 agccatgttg gtgtaaccaa tgaatcagtc gctgagctcc tttgatgggc ctaacaccat
    51001 cacgcgccac aatcgcttgt cgacgtctgt tcatctcagc aatatactct tttactgttt
    51061 tcggtaagcc aaactcctct ttcatagctt gccacatgaa ttcaaaagta gtccccatgt
    51121 actggtattg atacgagacg tccgtatcaa taccctcctc acgtaacatt tctgtcttat
    51181 tatctaaaaa cgtgtactcg gaatcaacaa taacgccgtc catatcaaaa ataatgactt
    51241 tttccatctt aaacctgctt tctatcccct ttattgtaat cgtttgcctt tctattttca
    51301 ataaaaaaag gttgctttct gtgaaaagca accagtatat ttagaaatga tattttgaga
    51361 gtgattttat ttaaaagggg agctcctctt cttccaatac taaatctgac aaatcaccag
    51421 aaacgttatt ttcacgcata gcacgttggg cacggctttc tagcaactga aatgatgatg
    51481 ctaatacttc agtgatatag tgcgtttgac catccttttc gtacttgcgc gtacgcaatt
    51541 caccatctat tgaaattaaa gagccctttg tcccatagct cgcaagggtt tccgctagac
    51601 gaccccacat aaccacatta ataaaatctg cttcacgctc accattactt cctttaaagc
    51661 gtctattaac agcaacagtt gcacgcgtca ctgacttgtc agttggtgtt tttaccatct
    51721 caggctttgc tgttagacgc ccaatcataa taactttatt atacattgat tacctcctca
    51781 ctattattat tcgaaaataa ggaagaaaaa tgctaaaata tatgaaattg ttctgggaga
    51841 attaagatga caaatgtaac tagttataaa gaaatgctgg ctaaaccttg gggaaaaata
    51901 caatatgaaa taacttttgc tcaattaagc catatcaaaa atcagaacgt tttagatttt
    51961 ggagctggtt tttgtctcac cgaacaacat ctagcaaaag aaaacaatgt aactgctatt
    52021 gaacccaatc ctaaactgct ttacgataat caaagtgaca atatttataa aatcctaggt
    52081 tcctacgaag cattaagaga tttgcctgat caaagctttg atactattat ttgtcataat
    52141 gttcttgaat atatagacaa acataatcat cctgcttatt ttgacgaatt ttctcgactt
    52201 ctaaaaccca atggagaact atctctcatc aaacataata taaccggtaa aatattacaa
    52261 tcagttattt ttagcaatga tacctccact gcgatggaac tcttaacagg agaagcaaac
    52321 tttaaaagtg ccagttttga ccagggaaat atttatacct tagaagaact aaaacaaaat
    52381 acaaatttat tagttgaacg ttatcagggt gttcgtacct tctattcatt acaaccaaac
    52441 cattttaaaa ctgaaactgg ttggctaaac aaaatgcttg ctatcgagct aagtgttgct
    52501 gacaaagctc cttataaaga tattgctttt ttgcaacaca tcacacttaa aaagtcattg
    52561 taacttctta agtttacaac taaaaatcaa cttagctgaa gctaagttga tttttactaa
    52621 tttttccaat gtttattggc gtcgaaagac tctccaacaa ttacctgatc agataattca
    52681 atgatacctc tgacttgagg agcatttgga agagctaact cgcgagcact acataacatt
    52741 ccaaagctat cttctccacg aagttttcct gggaaaataa ggctaccatt tggcatcata
    52801 gctcctggaa gagctgcaac tgttttaaga cctactgaag cattaggtgc cccacaaacg
    52861 atttggaccg tcttcccatc gttgatttct gcttgacaga tatgtaaatg gtcactatct
    52921 ggatgatcaa tcatttcttt aatttgcgcc acaacaaatg taggttcaat attattaaca
    52981 agagagtctt cgaatccttc tttcagtagt tctgcattaa ggattttaat atcttgatca
    53041 gataaagtta tttgacctgc tccctcaata acaattaaac ttgacacttc aaagatattc
    53101 caagcaactg tttctttact atcttgcaga tagacgcgtg ctacttgtcc gcgacgatca
    53161 acatctagct tagctccttg actatcctta acaataacca ttaaggtatc accaacatgc
    53221 tctctattat aagtaaaaat catttattct cctttaaatt ttgctatttt ctgtcttatt
    53281 tatagttaat ttcagcgaga aatttagtaa tttcagcctt tgttttgcga ttcttattaa
    53341 ccaaacgacc cagttcctga ccattttcca caacaacgaa actcggaatc ccaaaaatat
    53401 tccactgctg agctaattct atatagtcat cacgattaac acgtacaaag acaaaatctg
    53461 agaaatcttt ttcaattgat ggcattactg gatatataaa ttgacaatct ggacaccaat
    53521 ctgctgtaaa aaagaaaaca actttcttag tgctatcaat ataggctgct atttcttcat
    53581 aagattctgg taaaatcatt tgctcacctc cacataatca agaacacctt gacgataaat
    53641 gaattcgaaa attctgccat cttccaagac aagaccacca ctggtcattt taatatcaga
    53701 ttcaaattca ttaaaataaa atacctgaat atcacccatc tgactaaaaa attctcttaa
    53761 ggtctgagtg atttgctgac gtttataatc tgttactttt ttagttaggg tataaccagc
    53821 agctgccagt aaacccacac cagcaaggcc agtcccaaat aagatttttt tatttttcat
    53881 aactgttatt ataccatacc ccccggaatt agacacagga attgatggga gaaatccaca
    53941 taacagttat gaaaaaaaac aacttattcc ttgtggtata ataggatgac attgtcttaa
    54001 gataccatta tggttaatca ttcaaacaga aagaaaaaac atgaaagaaa aattattagt
    54061 acctgcttta aattgtttgc taggtctact cttcctaggt gatcactggt ttttatttcg
    54121 tcttcaaaat ggagaacttt ttgctattgt gacgctaata ctgctttgca caacttcatt
    54181 tttaatcaat aagaatcacc ccaaaaattt gactttaatt gctatggtta tcactttttt
    54241 agccgtatta tcttttctat ctaccttttt attagcttaa gccgataaac attatctccg
    54301 ttgtattcaa cccagttttc actataatag gctttgtcag tcataacagt cttccggtag
    54361 tacttgatat tctttttgaa agtctttata ttgactgaaa cctgttctgt tctttatttt
    54421 taattaaaag gtgctaaaat agtatttgta gaattgaggt aattatgtca gatttattta
    54481 acaaaattaa aaccgtaact gagcttgatg ggattgctgg ctatgaacac aatatccgca
    54541 acttccttcg tcaagaaata actcctttag ttgatcaagt tgagacagac ggacttggtg
    54601 gaatttttgg agttaaaaat actcatgaga ctaatgctcc taaagtcatg gttgctgccc
    54661 atatggatga agtcggcttt atggttagtc atattcagcc agatggaaca tttcgtgtac
    54721 ttgaggttgg aggatggaat cccctagtag tcagctcaca acgctttacc ctctacacac
    54781 gttctggtga tgctattcct gttatatcag gctcagttcc tcctcacttt cttcgtggac
    54841 aaagcggtgg aacaacatta cccaaaatta gtgacattgt ttttgatgga ggattcacag
    54901 ataaaaatga agctgaaagc tttggcattg ctcctggcga tatcattgtt cctaaatctg
    54961 aaaccatttt aactgcaaat caaaaacata ttatgtcaaa agcttgggat aatcgctatg
    55021 gtgtgcttat ggtgaccgaa ttgctaaaaa gcttaaaaga tcaaagtctt agcaacacac
    55081 ttattgctgg ggcaaatgtt caagaagaag tcggacttcg tggcgcacat gtttcaacaa
    55141 ctaaattcaa cccagatatc ttcttagctg tcgattgttc cccagctgga gatatttatg
    55201 gggaacaagg caaaatagga gagggaacct taatccgttt ttatgatccc ggacatatca
    55261 tgcttaaaga tatgagagat ttcttactta caacagctga agaagcaggt ataaaatacc
    55321 aatattatgc tgcaaatggt ggtaccgatg ctggggctgc tcacctaaaa aatagtggta
    55381 ttccttctac aactatcggt gtctgtgcac gctacattca ttctcatcaa acactctacg
    55441 ctatggatga ttttctacaa gcacaagctt accttcaggc catcgttaac aaattagacc
    55501 gctcgacggt ggatattatt aaaggttatt aagatagaaa ggatgggagt ttcataaggt
    55561 agacacattt tagtatctcc tttcatcccc atatcaacat tatgaaaatt ggaattatcg
    55621 gcgtcggaaa aatggccagt gcaattattc aaggcctcaa acaaacccaa catgacatta
    55681 tcatatccgg atcttgctta gagcgttcaa aagaaattgc agaaagactt gatgttactt
    55741 acgccgaatc tcatcagtca ttaatcaatc aagctgatat tatcatgtta ggtattaaac
    55801 ctcaactatt tgaaaaggtt cttcttccac ttgatatcac aaaaccaatc atctcaatgg
    55861 cagcaggaat ctccttagcg cgtttaagcc aattaactcg ttctgattta ccattgattc
    55921 gtattatgcc aaatattaac gcacaaatac ttcaaagttg cacagctatt tgttacaaca
    55981 atcatgtatc agatgagtta cgacaattag ccaaagaaat tacggatagt tttggaagta
    56041 gctttgatat agcagaaacc aattttgata ccttcactgc tctagcaggt tctagccctg
    56101 cctatatcta tctatttata gaagccttag ctaaagcagg tgtaaaatat ggatttccaa
    56161 aagaacaagc actctctatt gtaggacaga ctgtcttagc ttctagtcaa aacctattac
    56221 aaggacaaaa tagcacctct gacttaatcg acaacatttg tagcccaggc ggaactacta
    56281 ttgctggcct tttagactta gaaaaaaatg gtctgacgca tagtgttatt tctgccattg
    56341 atgctactat tgagaaagca aaaaaacttt gagcaaattg ctcaaagttt ttttagttat
    56401 tcatcattgt actgattaac acgctataag caagagcatt gttaataaaa tgaatcatta
    56461 tactatattc taagtgctca gttttataat aaacaaaaga taaaatagca cccatcccag
    56521 cataaataat aaaactacct atatcagaag gggaatgaat aagagcaaaa gctaaagtac
    56581 cgatgacaaa accccatttt tgatgtttcg caaaaagttc tttagggata atatagcgaa
    56641 atataatttc ttccataata ggggcaccaa ttatggcaaa aattaacatc aaagctagtg
    56701 gaagttgctt tgttaattcc ataattactg tttgatttgc tgtatcttta accccttgca
    56761 tagtcataat gaggtaacca ataatttgtg gaaccctcaa tgataaccaa cctaagagaa
    56821 tagtaactaa tgctttcctt gtcaataatg ctttccatct gattatttgt ttaaccttgg
    56881 cataataaag gaaaagagca ataataagta attctaagcc aatcaacaat atactagtaa
    56941 aaggcgtttg atagtgattc tttaggagcc acacagccaa tataggaaca agttgattaa
    57001 taactaaaaa tgctattagc aaagctaaaa ttttgccttt tctaataata aatgacacaa
    57061 aaacctccaa aataaattat taactttttt ctatcataac atattttcaa atcttataga
    57121 cttatctcac aataacttct gtaaaataaa gtaaattaat aaataaccat ataaaataaa
    57181 agagagtatc aatagcaaga actttgcata ggaaaaagtt ataccaaacc agacattaat
    57241 cagacacaat accactaaaa caaggacaga taaaaggaaa agaagtcgac aagacatatt
    57301 agcaataaat tgattacgct catcaaaaac tttggtatag taatgttgaa atcgcttttc
    57361 actaaatgtt atcaccaaaa aataaaaact tcctgcaaat aaggcaatac ctgaaccaaa
    57421 ggaaaaacct ttagtaaaat tagattcaaa ggtatgtaga aatgatgata ctatcataat
    57481 caaactacat acactaaaaa caaaagacaa aactctcata tatactttgt aatccttcgg
    57541 agatacttta ctattgggtg acaataatcc ttttattaac atagaatact actttctaaa
    57601 acttcaattt aatcatcagc tattatatcg ttttcttcaa tgaagcatag cgataaatag
    57661 caaaaataga catcgcaatg ctaatactaa tagtaatgat aaaaacatat tgctggtaaa
    57721 cgattggtgg acaaaaatca atagtccaca tccttgacat attacagatt aacttttaac
    57781 cttttgtctt ttgtggactg tatttttcca ttaaaattct atcctccttc actttctgta
    57841 taaataaaaa cttcctcaat ttgtttatca aaataacgcg ctatcttaaa tgctagctcc
    57901 aaagatgccg tgtatttctc tttttctaga gaaataatag tttgcctagt aactccaagt
    57961 gcaactgcaa gttctgcttg acttaatttc ctagattttc gtaacttttg tagagaattt
    58021 ttcatatctt acaccttttt tgtatagtta gctttacatt catagtatag ttaactatac
    58081 attttttgtc aagtacattt tacatatctg ttcaaaagac aaaaaagttg atagttaaac
    58141 tatcaacttt tctggtctta tcaatttttt gataacttat ttagctttaa ggcgctcaac
    58201 gtcacgcgca atcattaatt cttcatcagt aggaataact aaaaccttca cttttgaatc
    58261 tggttttgtg atatctccaa aataaccaaa aacatttttt tctggatcaa gttcaatgcc
    58321 aaaccatgat aatcctgcga taacatcttg acgcattaat ggcgcatttt ctcccatacc
    58381 tgcagtaaaa ataatagcat ccgcgccatt taatactgcc aagtattgtc caataaattt
    58441 cttaatacgg tcaataaaga cattataggc taacactgca tttggatctt tactttgaag
    58501 acctgcttca atatctcgca tatcacttga agttcctgat actccaagca atcctgattg
    58561 tttgttaagc atattaacaa cagctgctgc atcttccaat tctggatcat tagctacaag
    58621 ataagggata atcgcaggat caatgtctcc tgaacgtgtt cccatcattg gtcctgcaag
    58681 tggtgtgaat cccattgatg tatcaataga ctgtccatga taatttgctg taatagaaac
    58741 accattaccc acatgtgctg tgattaattt taattcttct aaaggacgtc ctaattgctt
    58801 agctgcttct tgtgcaacgt attggtggct tgtcccgtgt gcaccgtatt tacgtacttt
    58861 ataatcagta tagtactttt gtgggattgg atataagtag gtatgaggtt gcattgttgt
    58921 atggaaggct gtatcaaata cacatacact cgtgatatct ggtaagattt ctctaaacgc
    58981 acgaatccct gctgcagctg ctgggttgtg gagtggagct aaagctgata attcttcaac
    59041 ctgttctaca actttatcat ctactaaagc agactcttta aaatattcac caccagcaac
    59101 gacacgatga ccaacacctg taatttcatt aaagtctttg ataataccat gttttgttag
    59161 atcttctaaa agaattttaa cagcttgggt atgatctacg atatctaaaa tttgttcatc
    59221 ttttttatcg tcaaatttta cagtcgaaat agaatctttc aaaccaatac gttcgataat
    59281 ccctttggca actactttct cctctggcat ttcatataat tgccatttca aacttgaact
    59341 tccagcatta attgctattg tttttgacat aatttacctc tctaaagcgt tttcacaatc
    59401 tctattttat catatttttt gagtaatgac tattttctct gctccatttt tgaaaatttt
    59461 caatgaagtt tgccatattc tcacgatttt gcaaatctgt cagcggatat acaaaggttt
    59521 ctggtttttg ttctgcttgc ttcttgagaa caaatataga tttcgcattt tgacgacttc
    59581 caaaaatagt ttctggtaga gttaaaacgg caatgacatc tgcatatcct tttaaccact
    59641 ccttcagcaa atcactttgt ggacttgtta aaaggttttc gggtgctaaa aatatagcga
    59701 ttccatcttt tttcaaatat ttaagagatt gctccatcaa tagatggtga gcataggtgt
    59761 gctctttaga acttgataca gcatatcgtt tagcaatacc atcattagga taatagccaa
    59821 ctggtaaatc actaatgatt acatcgcttt ctttcaaaat ttgtggtcta acagcatcct
    59881 cttggataaa ttgggcacta gaacctataa tttcagcaat gcttgctgat agatcaatca
    59941 aaagatcatc aacttcaatg cccatataat ttaactcttt cgagctgtta ttgaggagag
    60001 tctgagctaa attcccagtt ccacttccaa tttccaagac atccactgtc tcttgactcg
    60061 ttaattcttc caaaagaaat aacaagataa aaccaatact atctggcgta aactgatgat
    60121 tagcttgtaa ttgctctgtt tgcgcagatt tgataaaaat gaactggaaa gtgcgacgcc
    60181 attcttcttg acttaagtca agttgacgta atttttggtt attcaccaca accatatcta
    60241 aatcacaact tgaaccaagg taataagagt tctgttcaat taaggcatca taaatatgag
    60301 tttttaattg gttctcaatc gtttggatat tttctaaaat cagctcatag gctgtctcaa
    60361 ttttttcaaa attcatgatt tcctccagaa tttatcataa caaaaaacac agcatctgaa
    60421 aagagaagca aacaaaaatc ctgtttttaa gcaaaacctt gtcttactaa tatttcatca
    60481 ctgtatattt ttaatgttta tggcttgctt tattatcatt agacttgaga ggtacttgga
    60541 gagtgtctgt tttacgataa aacttatggt tatagtttcc tcccttatcc aatgtaacct
    60601 taacttgtaa atagttccct tcttggaggt atgaagcata tcctcctgta aaataacatt
    60661 ttcctgatat ggagtcacct tctattgttt tataagctaa ctgcgctgtt aaataagctt
    60721 tacttgcttc aatttggcta tgatactgtc tttctgttgc caatattctt gctagataaa
    60781 actgtaagac gagtgtaaat acagcagcca atacaatagc ttgcaaaagg atgccagctt
    60841 taagtttctt ctttaaaatc atagtaaaat gtccttttta acccgtcctt aaaataaaaa
    60901 acaagtttta ccatactttt ggtctgactc atttgacaat tgtctaaccc ataaaccatt
    60961 ggttgataac ctcgaccatc ataacctgtc ttacggaaat catctttatt agatttgcca
    61021 aaggttacaa tcttatcttg cttacgtaaa taaagtttgt tctgtcttaa atattccaga
    61081 tgagcgcctt caaattctgc atttagttgc tgagttaata acacccattc agactgactg
    61141 gaagaagaca tcactactat ctgttgagcc aacaattttg tcagtccttg ataaactagt
    61201 aaagctcctg tgattgtaac caatgctacc aaacattcta aaagtgtaaa tgctttaacc
    61261 ttcttatcct tacattttaa caataaattt ttcatgataa tatctccata ttttgataca
    61321 ttggtaacag cattgctgca taaatcataa caatgataag agctacaaaa ataaaaataa
    61381 cgggttggat taactgggtc gctctagcta attttgtaaa aaaatcctcc cacttctcat
    61441 cagcatatat atctaactct gttcctaatt tcgccttaac ttggccatat tcaatcatta
    61501 aactaagctc agttaagaaa aacggatagt ctaatacttt ttggtgaaat gctttacctg
    61561 atagaaaacc ttcttccatg tcatatccta tttccctaaa aagtttggat ttctgatttt
    61621 gcattacttt aacaatttgg tccaattcaa taccttgact taataaattt ccccactcac
    61681 gggcatagta agcagttaaa taaagcttaa catatgatcc aactaaggga attgttgtta
    61741 aaaaacaagc tactttaatg cgcgacaatc gcttttgaat aatataaaat attaaactaa
    61801 aaataagtac aactgctaaa agtaataaga aaatattcgg cacatttgta atcagtctag
    61861 ttgcaaaatt attttctcct aattggggca ttaaataatt cctaaggcca atcataatta
    61921 gcaccagaaa agacaataat atcaatgggt aagtagctac ttctattact ttttttctaa
    61981 ctaacaaaag attagctaaa taagactcaa tctttagtag gctccttgaa atgttaccat
    62041 gcaaatcagc taatgcaacc tgtgtgataa cagtgtcaga aaaacctaac tctcctaaca
    62101 tttttgataa atcttttccc tctaataatg ccttattcat actatctgta taacaatcag
    62161 acaataactt actcctcttt aagaaggtga ccatatcagt taaagaaaaa ccactagcaa
    62221 aaagattatt aaaaagttgg actactttcc gttgtttctt gagggataat tttttcgact
    62281 tgtgcctgtt tcttactgat atgtccttct tcagccaaga tatccacttg tctattccac
    62341 ttgtctgatg agtgtttttt aaaattacct gtctcaaagt caattaggct tcctcctcca
    62401 attaaacgtt gatatgctat taattttaga ctattttcta actcttgata gttaacccct
    62461 aattctataa gcctatcata gactccggga atacttttag catgaatagt agaaaaaacc
    62521 atcactcccg ttaaacttgc acgaataaca gcacgggccg tcgcttgatc tctaatctct
    62581 ccgataatta aaatatctgg acgatgccgt aaagacagtt tgattaaagc atcataagtc
    62641 attccaatat cctcattcaa ttggagttgt aacatcttgt cattcttgat ttctaccgga
    62701 tcttcaatcg tgataatttg cttattttta aatacttctg aagctaattg atacatgaga
    62761 gttgttttac cactccccac agggccggaa aaaagatata gccctcttat acccagtact
    62821 tccttcattt gctttatatt atcaaaccaa tattttaagt cctgatgacc tgaatacaaa
    62881 atacgaataa ctaaagattc ttgaccacga taatctccca cactcgatag tcgtaatgaa
    62941 accagtcttc cctctgacag ttcatagtca caagaaccta attgacttcg tcttttttct
    63001 ccaacgttca tgcctgccac aaatttaaag tgactaataa gactagccat cctattaaac
    63061 tcaaaaacat caataaaccg cctttcatca tcaatacgca tatagagttc ataacaatca
    63121 cctttgggaa tgatataaat atcttgagca tttacttcta ctgcctgatg aatgacttgc
    63181 tttgctaatg attgaaccat cccgtaacct ccttaaccat atattcgaaa ataaaataaa
    63241 aaaatctcac caattggtga gatcttttca atgatttctt gagaggtagt aacataaata
    63301 ttatgaagca gaggctgaac aaacctatta tatccccata aaaactatct aactttttta
    63361 aactactttt aattacaaat ttaaagaaca tgccgatggc aactcagcta tcttattaaa
    63421 tacttcaaat cgctcaatag attcttcctt aggaatttct tgccattctt tcaataacat
    63481 taaggaatga tactgttgga ggtactcatc acattcttca caccaacgtt tttcttttat
    63541 actccaaaac gttgccaaca aactagactt actttgaaaa taaggccact tttcctgtcc
    63601 cctatcccac tcctcttgaa aatagagcaa agcttctgat aaggtatcat actcagcaat
    63661 ttcagtaata tcttcctccc aaccttcaat aaaccaccat ggttcccaat caccaaacat
    63721 ttttacaact tgatacataa tacacttcct ttagagagta ttatataaaa aataagaata
    63781 aaataaaaaa tatctgctca ttccaaattc ttgtaaaaaa agaacctgag ttaaactcaa
    63841 gttctttttg attaatcttc tgttgtaggt gttgatactt ccgtaacttc tgcatcaaca
    63901 ggagttcctt caataatctc aacttcatta acagcaagtg gttcaatatt acggtaacga
    63961 gccataccag tacccgctgg aatgatttta ccgataataa cattttcttt aagaccaagt
    64021 aagtgatctt tcttaccacg gatagcagca tctgtaagga cacgtgttgt ttcttggaag
    64081 gatgcagctg ataagaatga attagtttca agggaagcct tagtaatacc cataaggaca
    64141 ggacggcttg tcgcagggat tccaccagaa ataacaatat ctttgttagc atctgtaaag
    64201 tctgaaatat ccataagtgt acctggaaga agatctgtat ctcctggatc catgacacga
    64261 actttacgga gcatttgacg aaccattacc tcaacgtgtt tatctccgat ttctacccct
    64321 tggctacggt aaactttttg tacttcagca agtaggtacg tttcaacaga taaagtatca
    64381 cgaacttcaa ggagacgttt tggttggata gatccttcag ttaaggccgc accacgagct
    64441 acttcatcac caacttcaac tttcatacgt gctgtaaatg gaacaacgta ttctccctca
    64501 cctgtctgac ctttaacgaa tactttctta gtacgtgttg aagagtcttc ttcaattgca
    64561 acgacttcac ccttaacttc agtaataact gcctcacctt tagggttacg tgcttcaaag
    64621 atttcttgga tacgtgggag accttgtgta atatcagtat ttgaagcaac accaccggta
    64681 tggaaggtac gcattgtaag ctgtgtccct ggttcaccga tagattgagc tgcaatagta
    64741 ccaactgctt caccaacctc aacagcatca cctgttgcca agttaatacc gtaacagtga
    64801 cgacaaacac catggcgagt gttacatgta aatactgatc ggatagttac ttcctcaacg
    64861 cccgctttaa caactttagc agccatatct tcagtaatta aggtatctgc accaacgaga
    64921 atttcacctg tctcaggatg tttgattgac ttcttagtat aacgtcctat caaacgctct
    64981 tcaagtgtct cagtaacctc tttaccatct gtaattgctg tgattgtcaa accgcggtct
    65041 gttccacagt catcttcacg aataataaca tcttgggcaa cgtcaaccaa acgacgagta
    65101 aggtaacctg agtcggctgt cttaagggcc gtatcagtca tacctttacg cgcaccgtga
    65161 gttgagaaga acatttccaa aacactaaga ccttcacgga agtttgataa gattggcaat
    65221 tccatgatac gtccgttagg agcagccatc aaaccacgca taccggcaag ttgtgagaag
    65281 tttgagatgt taccacgagc tcctgagtcc atcatcataa cgatagggtt ctttggatct
    65341 tgtgtctcaa taagacgttt ttcaagtgct tctttagctt cacgccatgt tgttgtaaca
    65401 gcaacataac gatcttcttc agtcatcaaa cctcgacgga aggctttatt gatatcttca
    65461 actctgtggt gagctgcatc aataatctca gctttattgt caataactgg gatatcagca
    65521 atacccacag tcaaaccagc aagtgttgaa tgataatatc caaggtcctt caaacggtca
    65581 aggaaagcag aagtttctgt tgtacggaaa cgtttaaaag tttctgcgat aatattacca
    65641 agatttttct tcttaaaggg aatattaatc tctaagttat caataactgc ttgaatatcc
    65701 tgacctggct ctaagaagta tttatctgga gttttttctg tcaagtttgc attattaggt
    65761 tcaattaagt acggtaaatc ttcaggcatg atatcgttga agagaatctt accaactgtt
    65821 gtaaccatga ttttatgttt ttgctcttct gtccaaggct tattcggcat tgaatcaaca
    65881 gcgataccaa cacgtgtatg taaatgaaca taaccatttt gatatgccat aactgcttcg
    65941 tcatgatctt tgaaaatcat gccttcacct tcacgaccag catcttccat agtcaggtag
    66001 taatttccta aaaccatgtc ctgagatggt gttacgactg gcttaccatc tttagggtta
    66061 aggatgtgct ctgcagcaag catcaaaaga cgagcttctg cttgggcttc ttcagacaat
    66121 ggaacgtgga tggccatttg gtctccatca aagtcagcat tgtaggcctc acatacaagt
    66181 gggtgaagac gaagcgcctt accatcaata aggacaggtt caaacgcttg gatacctaat
    66241 ctgtgaagag taggtgcacg gtttagaagt actgggtgct ctttaataac ttcttctaag
    66301 atgtcccaaa tacgctcgtc cccacgttca accatacgtt tagctgcttt aacgttccct
    66361 gcgaggtcac gagcaacgat ttcacgcatt acaaaaggtt tgaagagctc aatagccatt
    66421 tcacgtggta caccacattg gtacattttg agtgttggac caacagcaat aacggaacgt
    66481 ccagagaagt caacacgttt accaagcaag ttttggcgga aacgaccttg tttaccttta
    66541 agcatatggc ttaatgattt caaaggacga ctacctggtc cggtaattgg acgaccacga
    66601 cgaccattgt cgataagcgc atcaacagct tcttgtaaca tacgtttttc attttgaacg
    66661 atgatgccag gagcatttaa ttccaacaaa cgtgccaaac gattgttacg gttaataaca
    66721 cggcggtaaa ggtcattcaa gtctgatgcc gcaaaacgac caccatctaa ttgaaccatc
    66781 ggacgaagat ccggtgggat aaccggtaaa atgttaagaa ccatccattc tggtttattt
    66841 ccagattttt taaaggcatc taacacatcc agacgacgaa ctgctttcac acgtttttga
    66901 cctgtcgctg atttcaactc ctctttaaga actgcgattt ctgcatctaa atcaacgcgt
    66961 ttaagaagat cttgaatggc ttcagcaccc attttagcaa caaacgaacc gtaaccgtac
    67021 tcttgaagtt tttcacggta ttcacgctca gtaaggagtg acttcggttc taatggtgtg
    67081 tccattggat cgataacaac ataagccgca aaataaataa cttcttcaag tgcacgagga
    67141 ctcatatcca atgtgagccc catacgcgat gggatacctt tgaaatacca gatatgagat
    67201 actggtgctt ttaactcgat atgtcccata cgttcgcgac gaacttttgc acgggttact
    67261 tcaacgccac aacggtcaca aatgattcct ttgtaacgaa tacgtttgta ttttccacac
    67321 gcacattccc aatcttttgt tggaccaaaa ataacttcat caaaaagtcc ttcacgttct
    67381 ggtttcaatg tgcggtagtt gattgtttca ggttttttaa cttcaccata agaccatgaa
    67441 cggaccttac ttggtgatgc taatgtgatt tgcatacttt taaaacgatt tacgtcaacc
    67501 actagtttta cctttcttcg atttatcatc atactagccc agctaggatt tcaataaatc
    67561 ctaggactag cattgcatat caattctata ttcgcttcta gttataaact agataaattt
    67621 aatcttcttg aacgacttca gatacttgtt cagcttgttt ttcttcagct tcttgtttca
    67681 cacgtgcttt ttcaaggtca tcaacgtgca tcacatcatc atcttcacct tcatcaagat
    67741 cacggagttc aacttcgttg tcatcttcat caaggacacg catatcaaga ccaagtgatt
    67801 gcaattcttt tacaagaaca cggaatgatt ctggaacacc tggttttgga attggcttac
    67861 cttttgtgat agcttcataa gctttaagac gtcctgtaac atcatcagat ttgtatgtta
    67921 aaatttcttg aagaacattt gaagcaccat aagcttcaag cgcccaaacc tccatctcac
    67981 caaaacgttg tccaccaaat tgagctttac ctccaagtgg ttgttgagta acaagtgagt
    68041 atggtcctac tgaacgggca tgaagtttat catcaaccat gtggtgaagt ttgatcatgt
    68101 acatgacacc aactgacaca cggttatcga atggttcacc agtacgacca tcataaagaa
    68161 cagtcttagc atctgaatcc atacctgctt cttgaacagt ttcccaaagg tcttctgaag
    68221 aagccccatc aaagactggc gttgcaatat gaatacctaa attacgagca gccataccta
    68281 aatgaagctc cataacttgt ccgatattca tacgtgatgg caccccaagt gggttcaaca
    68341 tgatatcaac tggcgttcca tctggtaagt aaggcatatc ttcaacagga acaatacgtg
    68401 agacgacacc tttgtttccg tgacgaccgg ccatcttatc accgactttg attttacgtt
    68461 tttgagcgat ataaacgcgg acaagcatat taacacctga ttgcaattca tcaccatttg
    68521 cacgagtaaa gattttaacg tcacgaacta ctccatcgcc accgtgaggt acacgtagtg
    68581 aagtatcacg aacttcacgt gatttatcac caaaaatggc atgcaagaga cgttcttcag
    68641 cagataagtc cttttcaccc ttaggtgtta ccttaccaac aagaatgtca ccttctttta
    68701 cctcagcacc aatgcggata attcccattt catcgagatc acgtagtgaa tcttcaccaa
    68761 catttggaat ttcacgagta atttcttcag gcccaagctt tgtatcacgc gtttcagatt
    68821 cgaattcttc caagtgaaca gatgtataga catcttcttt cacaaggcgc tcactcatga
    68881 taacggcatc ctcgaagtta taaccttccc aagtcatata agcaacaact gggttttgtc
    68941 caagagccat ttcaccattt tccatagatg gtccgtcggc gataaaatca cctttttcaa
    69001 cgagatcacc aactttaact aaggtacgtt ggttataagc agtacctgag tttgaacggc
    69061 ggaatttttg aacatgataa acatcaagag aaccatcttc gcgacgcact tcaacttttt
    69121 cagcatctga aaaaataaca cgaccatcat gtttagcaat cacagctgca cctgaatcgt
    69181 gggctgcttg atactccata ccagtaccaa catatggtgc ttttggatca atcaatggca
    69241 cagcctgacg ttgcatgttg gcacccatca aggcacggtt agagtcatca ttttccaaga
    69301 aaggaataca tgctgttgcg acagcaacta cttgcttagg tgacacgtca acaaagtcaa
    69361 caatgcttga aggaaactct tggttattac cttgatgacg acccataacg atttcttctg
    69421 caaatgtacc gtcttcgtta agcttagagt tggcttgtgc aactgtaaat tcatcttctt
    69481 catcagcagt caaccaaaca atttcgttcg ttaccgcacc agtagaacga tctactttac
    69541 gatatggtgt ttgaataaat ccatatttat tgaggtgtcc aaatgaagat aagttattaa
    69601 tcaaaccaat gtttggacct tcaggtgttt caatcggaca catgcgccca tagtgggtat
    69661 agtgcacgtc acgaacttca tatccagcac ggtcacgtgt caaaccacca ggtcctaagg
    69721 cagagagacg gcgtttgtgc gacaattctg atagagggtt gtgttggtcc atgaattgtg
    69781 acagctgtga tgaaccaaag aattctttaa cggcggcagt cactggacga atgttaatga
    69841 tttgttgagg tgtcaagact tcattgtctt gtacagacat acgttcgcgg acattgcgtt
    69901 ccatacgagc aagtccgata cggaattggt tcgcaagtaa ttcaccaact gcacgaatac
    69961 gacggttacc taaatggtca atgtcatcta ccttaccgat accctctgca aggtttaaga
    70021 agtatgacat ctctgctaaa atatctgcag gagttaaggc acgaacttta tcttcaggat
    70081 ttgagtttcc aacaatagta acaacacggt ctggatcagt tggagcaaca actttgaatt
    70141 tttgaagaat aactggttca gtaactacag cataatcatt tggtgtgtag acaaacttgt
    70201 ttaagtctcc atcgatatgc tctgcaattg aatcaatgac atctcgtgtc ataacggttc
    70261 cagcttcaac aagaatctct cctgtttcac catcaactaa attttcagcg attgtttggt
    70321 tcaataggcg tgttttaaga ttcagttttt tattaatctt ataacgacca acagccgcta
    70381 agtcataacg acgtggatca aagaaacgtg caactaaaag actacgtgag ctgtctgcag
    70441 tttttggttc acctggacga agacgttcat agatttcttt aagtgcttca tctgtacgag
    70501 aatcacttgg atttttatga atgtcttttt caatagtatt gcgaacaagt tcactgtcac
    70561 caaagatgtc aacaatttca tcgtctccag agaaaccaag tgcacggact agtgttgtaa
    70621 atggaatttt acgtgtgcgg tcgatacgtg tataagcaat atcttttgcg tctgtctcaa
    70681 gttccaacca agctccacgg ttagggataa cagttgaccc ataaccaact tttccatttt
    70741 tatctacctt atcattaaag taaacaccag gagaacggac taattgtgaa acaatgatac
    70801 gttcaccacc attgatgatg aaagtaccca tttcagtcat aattggaaaa tcaccaaaga
    70861 aaacttcttg agttttaatt tcaccagttt ctttattaac taaacggaaa gtaacaaaaa
    70921 taggtgctga gtagctagca tcatggatac gagcttcttc caaagtatat ttaggttctt
    70981 ttaattcata accaacaaat tcaagatcca tagtatctgt aaagtttgaa attggaagta
    71041 catcctcaaa tacttctttc aagccagcat caagaaaatc ttggaatgaa tcagtttgaa
    71101 tttcaattaa gtttggtaaa tcaagaactt ccttaattct tgaaaagcta cgacgtgtac
    71161 ggtgttttcc gtactgaact tcatgtcctg ccaagttttt agctcctttt taaagataga
    71221 gtgactaagt ttttatctca taaaacttag tgagtttatt tcatttgctc gcctaacaag
    71281 aacaagctta tagatgtcgt ttgtggtaat tttcagaaag tttaaatata tattacaata
    71341 accttttttc ttaaaattag gcacaaaaag agcagctaaa tctcacttta tcaagttgat
    71401 ttaactgctg tatatctagg tcggacaata tttcaaccta gacagacgac aaatagttac
    71461 gtacattata acttattttc accaataaat caagttttat tctcttgact tcaaggaagc
    71521 ttctagaatt tcaatttgtt catcaacacc cttgagtttt agcataacac gatgattttt
    71581 aaacatagca tactggtgtt tttctaattc tttgaaagaa gactatacaa gaattaaatc
    71641 atattatctt ggaaaaaata aaaggagaac agatcactct attctcctca aataaaacta
    71701 tcagtatcgt aaacctgtta tcgttttcca cccaaagtag accaagcttt ttgataatca
    71761 ctatcagttc ccccaatagc aaaacgatag gtcatcgttc caggtccatt ttttgcccaa
    71821 taacttgttg tgctttcacc tccaacagtg attcgacgac cgtttactgt tactacacct
    71881 ggctgtaaac ctgtagactt aagaacttta gcttttataa cgctatcatc aagtctaaac
    71941 ctttctgact taccaaaagt attgccatct gcattattaa tagcattaac caagtgagcc
    72001 atataattag cattattatt gtaacctgtc aatttcgcta aagaggcatt attatcatgt
    72061 cctgcccatc cacctaaagt cactttaggt gtagaaagca tcaaccaaac atcagaagtt
    72121 gagttagtag tacccgtttt tccaatccaa tctaccccag ctaaaccact atttaaacct
    72181 tgtaagcgat ttttaaaggt tgtagtcttt ccggagttaa ttggtccatg taatagctgc
    72241 tgtaagatag tagctgttgc tttcgaaaag acacggacag gtttactttc gtgattataa
    72301 atcactttcc cattactatc ctctatactt tctatcatat actgcttatg ataaacacca
    72361 ccatttgcaa tcatttgata aaggtttgtt tgctgagcaa ctgacgtatc aataccacca
    72421 ccaagtggta aactttctat accaaagttt tcaattggat agtctaattt ctccatatag
    72481 ttttttacat caacgcctcg atctcgtagc atcttgtaag tccagaaagc tggaatgttc
    72541 caagaaatgt ccaatgactc ttgaaggtta accatagcag taccttcttc atccgcatgc
    72601 ataatctttt cgccacttga atatgtcgtt ggataatttg aaagaacact accacttcct
    72661 aacatccctt gatcaatagc aattccgtaa ggtaatatcg gtttaatact tgaccctgga
    72721 gagcgtgccg tatcaaaagc atggttattt tgattctcac tatagttacg accaccaata
    72781 aatcctataa tagcaccact agaattgtcc gttagtacat ttcccatttg aaccttgcca
    72841 gtaccatcat ctaacaaccc tccgtattga gctgcagcat cctgcatggc ttgataaaca
    72901 gatttattga tagtagtttt aatagtatac cctccctgtt gaatctcttc aatagcgcgg
    72961 tgtctgtacg tcgctctagt ctcatcattt tttaaatcat gctctgaaac attatccttt
    73021 ttaatgaggt aattgtacat cactttttga gcttcagata atgccgagta gtaaagataa
    73081 tcatgatgat ttgttgtcgc cactgcaggt ttgataaaat cttttttgat gtcataatct
    73141 ttgtaggatt tgtactcatc tttggttaaa gctctcgttc tatacatatt gtataacaca
    73201 tttttttggc gctttatacc aaaactaaga tctttatctg atttcaactg ggcatctgca
    73261 gtataaggag aatatacaat aggactttgc ggtaaacctg caaggaaagc tgcctgagga
    73321 attgttaaat cttttgcgga aactccaaaa atcccttgtg ctgcttcttc aatacctgca
    73381 atattctgtc ctttgttatt gcgaccaaac ggtgatacat ttaaataatc tgataaaatc
    73441 gaatccttat ccatgtaacg ctctaatgct agagcataaa taatttcttt tgatttgcgt
    73501 ttaaatgacg gatcatctcc caaaatttgc tgctttaata attgttgtgt cagcgttgaa
    73561 ccaccgctac tctctccaaa ccccagaaca gatcccgctg cagcacgtag tacggctttg
    73621 ggaactacac ctttatggtc attgaaattt tcgtcctctg ttgcaatgat agccttttta
    73681 atattatcag atatagcatc ctttgccact ggtgtacgtt gtaaatcagt ggcaatctct
    73741 gaaatttgtg atttatcaga ataagttaac ctagacaccc tagtaagtgt attaacttgt
    73801 gtcactaaac tatttttact tggtacttta acagagtcaa cttgactagc aagataccct
    73861 actgcaagtc ctactccgag cataacaaat agtaaaataa taacatagat gaaatctgat
    73921 aaaagcttta caattcttaa aaaaatagaa ccaaattcaa gtggtgtgta atcacctaat
    73981 ttagaactat tcaacttctt attaccttta aacatgaata atacctcatc ctacattata
    74041 tcagatttaa tataacttgc atatcctaaa gagttttgat atagttgaca ttaatacaca
    74101 acaagttaat aattaggaga aaaagatgaa tatttttgat gaactcaaag aacgtggact
    74161 cgtttttcag acaactgacg aagatgcttt acgcaaagct cttgaagaag gttctgtctc
    74221 ttactatact ggctacgatc ctacggctga cagccttcat cttggacatt tagttgctat
    74281 tttaacctct cgtcgcttac aacttgctgg tcacaagcct tatgcacttg tgggaggcgc
    74341 aactggactt attggggacc cttcatttaa agatgttgag cgcagtttac aaacaaagaa
    74401 aactgtagtt agttggggta ataaaatccg cggtcaatta tctaattttc tagagtttga
    74461 aacaggtgat aacaaagctg tattggtaaa taattatgat tggttcagca atattagttt
    74521 cattgatttc ttacgagatg ttggtaaata ttttactgtc aattatatga tgagtaaaga
    74581 atctgttaaa aaacgtatcg aaacagggat ttcctatact gaatttgctt atcaaattat
    74641 gcaaggatat gacttttacg aattaaataa gaactataat gtcactttgc aaattggtgg
    74701 ttctgaccaa tggggcaaca tgactgctgg aaccgaactt attagacgaa aatcaaatgg
    74761 tgtatctcat gttatgactg ttccattgat aacagattct acaggaaaaa aattcggaaa
    74821 atctgaagga aatgctgttt ggcttgatgc tgacaaaacc tctccttatg aaatgtacca
    74881 attttggctc aatgttatgg atgctgatgc cgttcgcttc cttaagattt ttacattcct
    74941 aagccttaaa gaaatagaag atatacgtat tcaattcgag gaagcacctc accaacgctt
    75001 agcacaaaaa acactagcac gtgaagttgt aactcttgtt catggtgaaa aagcttataa
    75061 ggaggccgtt aatatcacgg aacagctatt tgctggcaat ataaaaggat tgtctgttaa
    75121 agagctcaaa caaggtttac gtggagtacc aaattatcac gttcagacgg aagataacct
    75181 caatatcata gaccttctag tcacttcagg agttgtcaat tctaaacgtc aagctcgaga
    75241 agatgtctcc aatggagcta tttatattaa tggagatcgt attcaagatt tagaatatac
    75301 tatctcagaa aatgataagc ttgaaaatga aattacagtc attcgtcgtg gaaagaaaaa
    75361 atactttgta ttaaacttta aatagacttg aagtgtaatc gatactctga taataatatt
    75421 tcaagacaat aaaaagtgta gtcaattgac tacacttttt a
//
LOCUS       DATRMA010000003        65259 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.003, whole genome shotgun sequence.
ACCESSION   DATRMA010000003 DATRMA010000000
VERSION     DATRMA010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 65259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65259
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.003"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            <1..391
                     /locus_tag="VBC28_000156"
     CDS             <1..391
                     /locus_tag="VBC28_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEO6814166.1"
                     /translation="IDVVFFDKNISYEETVVLEQQLKDNYPQYDWELKNEFYMNTHSP
                     NTPKYTSSKDAISKFPEKCTAVGARLDDRNQLELYLPYGEEEILNFIVSPTPYFEEDL
                     LRYNVYLKRVDKKKWNNIWPRLTIIKK"
     gene            611..1030
                     /locus_tag="VBC28_000157"
     CDS             611..1030
                     /locus_tag="VBC28_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000784700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjdF family protein"
                     /protein_id="HEO6814167.1"
                     /translation="MKMTVYFDGNFWLGLIEYDDDGDYKVFRYFFGKEPKDDDVFNFI
                     NHKLNDLIKKYEFVKTDISLKRTNEHKKSPKRMQREINREKRKPVVSTKAQLAMKTIH
                     MSIKNERQLSQKCKKNELRKHRYQLKQEKRYQKKKGH"
     gene            1230..1709
                     /locus_tag="VBC28_000158"
     CDS             1230..1709
                     /locus_tag="VBC28_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEO6814168.1"
                     /translation="MRDFEELFDKVKPIVMKLRRNYFVQLWEYDDWIQEGRIVLFRLL
                     EEHPYLLDNESKLFIYFKTKFSNYLNDVLRHQDCQKRQFNKMPYEEISEVSHYVKSKG
                     LVLDDYIAYRDTLTKVEETLSDIDKEKFEKLISGERFAGKKQFIRDIQPFFNAFKAD"
     gene            1831..2523
                     /locus_tag="VBC28_000159"
     CDS             1831..2523
                     /locus_tag="VBC28_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000049270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEO6814169.1"
                     /translation="MSKVRLYIARHGKTMFNTIGRAQGWSDTPLTTFGELGIKELGLG
                     LKASNISFKEAFSSDSGRTLQTMEIILREVQQENIPYTRDKRIREWCFGSLDGGYDGD
                     LFNGVLPRVSNGDMSHLTHEEIANLICQVDTAGWAEPWAILSNRILSGFTAIAKKIED
                     IGGGNAIVVSHGMTIATFLWLIDHSTPRSLGLDNGSVSVVDFEDGTFSIQSIGDMSYR
                     EKGREILEKTLQ"
     gene            2520..3272
                     /locus_tag="VBC28_000160"
     CDS             2520..3272
                     /locus_tag="VBC28_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HEO6814170.1"
                     /translation="MKKNKIIRFSLVGVLLAILCFSLFALLKPNSQQSSSQKLRNEDI
                     KKISSQKRNKKLQLPAVSSKDWNLILVNRDHKHEELSPDVVPVENIYLDKRITKQATQ
                     FLEAARAIDSREHLISGYRSVAYQEKLFNSYVTQEMTSNPNLTRGQAEKLVKTYSQPA
                     GASEHQTGLAMDMSTVDSLNESDPRVVSQLKKIAPQYGFVLRFPDGKTAETGVGYEDW
                     HYRYVGVESAKYMAKHHLTLEEYITLLKENNQ"
     gene            3269..3844
                     /locus_tag="VBC28_000161"
     CDS             3269..3844
                     /locus_tag="VBC28_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEO6814171.1"
                     /translation="MRKRFSLLNFIVVTFIFFFFILFPLLNHKGKVDANSRQSVTYTK
                     EEFIQKIVPDAQDLGKSYGIRPSFIIAQAALDSDFGEKILANKYHNLFGLLAEPGTPS
                     ITLNDSSTGKKQEKQFTHYKSWKYSMYDYLAHIKSGATGKKDSYTIMVSVKNPKTLVQ
                     KLQDSGFDNDKKYAKKMTEIIDLYDLTRYDK"
     gene            3939..5021
                     /gene="hrcA"
                     /locus_tag="VBC28_000162"
     CDS             3939..5021
                     /gene="hrcA"
                     /locus_tag="VBC28_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEO6814172.1"
                     /translation="MCKSANFRKTERGDVFVITQRQNDILNLIVELFTQTHEPVGSKA
                     LQRTIDSSSATIRNDMAKLEKLGLLEKAHTSSGRMPSPAGFKYFVEHSLRLDSIDEQD
                     IYHVIKAFDFEAFKLEDMLQKASHILSEMTGYTSVILDVEPARQRLTGFDVVQLSNHD
                     ALAVMTLDESKPVTVQFAIPRNFLTRDLIAFKAIVEERLLDGSVMDIHYKLRTEIPQI
                     VQKYFVTTDNVLQLFDYVFSELFLETVFVAGKVNSLTYSDLSTYQFLDNEQQVAISLR
                     QSLKEGEMASVQVADSQEAALADVSVLTHKFLIPYRGFGLLSLIGPIDMDYRRSVSLV
                     NIIGKVLAAKLGDYYRYLNSNHYEVH"
     gene            5024..5596
                     /gene="grpE"
                     /locus_tag="VBC28_000163"
     CDS             5024..5596
                     /gene="grpE"
                     /locus_tag="VBC28_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEO6814173.1"
                     /translation="MAVFNKLFKRRHSVSEEIKKDDLQEEVEATETEETVEEVIEEIP
                     EKSELELANERADEFENKYLRAHAEMQNIQRRSSEERQQLQRYRSQDLAKAILPSLDN
                     LERALAVEGLTDDVKKGLEMTRDSLIQALKEEGVEEVEVDSFDHNFHMAVQTLPADDE
                     HPADSIAEVFQKGYKLHERLLRPAMVVVYN"
     gene            5777..7606
                     /gene="dnaK"
                     /locus_tag="VBC28_000164"
     CDS             5777..7606
                     /gene="dnaK"
                     /locus_tag="VBC28_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEO6814174.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGTESKIIANPEGNRTTPSVVSFKN
                     GEIIVGDAAKRQAVTNPDTVISIKSKMGTSEKVSANGKEYTPQEISAMILQYLKGYAE
                     DYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGMDKTDK
                     DEKILVFDLGGGTFDVSILELGDGVFDVLATAGDNKLGGDDFDQKIIDFLVEEFKKEN
                     GIDLSQDKMALQRLKDAAEKAKKDLSGVTQTQISLPFITAGSAGPLHLEMSLSRAKFD
                     DLTRDLVERTKTPVRQALSDAGLSLSEIDEVILVGGSTRIPAVVEAVKAETGKEPNKS
                     VNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTSK
                     SQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDIDK
                     NGIVSVKAKDLGTQKEQHIVIQSNSGLTDEEIDKMMKDAEANAEADAKRKEEVDLKNE
                     VDQAIFATEKTIKETEGKGFDTERDAAQSALDELKKAQESGNLDDMKAKLEALNEKAQ
                     ALAVKLYEQAAAAQQAAQGAEGAQSADSSSKGDDVVDGEFTEK"
     gene            7895..9034
                     /gene="dnaJ"
                     /locus_tag="VBC28_000165"
     CDS             7895..9034
                     /gene="dnaJ"
                     /locus_tag="VBC28_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEO6814175.1"
                     /translation="MNNTEFYDRLGVSKDASQDEIKKAYRRMSKKYHPDINKETGAEE
                     KYKEVQEAYETLSDTQKRAAYDQYGAAGANGGFGGFDGGGFGGFDGGGFGGFEDIFSS
                     FFGGGGMRNPNAPRQGDDLQYRVNLSFEEAIFGAEKEVSYNRESSCHTCSGSGAKPGT
                     SPVTCQKCHGSGVINVDTQTPLGTMRRQVTCDVCQGSGQEIKEKCPTCHGTGHEKKTH
                     KVSVKIPAGVETGQQIRLTGQGEAGFNGGPYGDLFVIINVLPSQQFERNGSTIYYTLN
                     ISFVQAALGDTIDIPTVHGAVEMSIPAGTQTGKTFRLRGKGAPKLRGGGQGDQHVTVN
                     IVTPTKLNDAQKEALHAFAEASGDKMVHPKKKGFFDKVKDALDVD"
     gene            9148..10395
                     /locus_tag="VBC28_000166"
     CDS             9148..10395
                     /locus_tag="VBC28_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEO6814176.1"
                     /translation="MVTKVEEIRSYLIASIQNGKLAPGDRLPSIRQLANQFFCNKDTV
                     QRVLMELRFDNYIYAKPRSGYYVFDSHQEEVEEGVSLPNSEIANIAYDDFRLCLNETL
                     IGREDYLFNYYYRQEGLLDLSKAVAKLMEETGVYVPLDDIVITAGTQQALFILTQVTF
                     PNRKSRVLIEEPTYPRMIELIKTQNLPYETISRGTHGIDFQRLEEIFQTQSIKFFYVI
                     PRMHNPLGTSYNPVEMKRLIEMAEKYDVYIVEDDYMSDFASQSPLHYYDTHGRVIYLK
                     SFSKAIFPALRLAAICLPQALKSTFMAYKKLMDYDTNLILQKALALYIENGLYAKNSQ
                     YLKYRYQKDLANSKSILADHPNLPSYSLHHDSVLFDCSKLDNFKILRQYGDTLENYFC
                     QKSHQSLLQVKNDSCLKQFLGSL"
     gene            10466..11242
                     /gene="truA"
                     /locus_tag="VBC28_000167"
     CDS             10466..11242
                     /gene="truA"
                     /locus_tag="VBC28_000167"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEO6814177.1"
                     /translation="MTRYKAQISYDGSAFSGFQRQPNCRTVQEEIERTLKRLNSGNDV
                     IIHGAGRTDVGVHAYGQVIHFDLPQARDVEKLRFGLDTQCPDDIDIVKVEQVSDDFHC
                     RYDKHIKTYEFLVDIGRPKNPMMRNYATHYPYPVIIELMQEAIKDLVGTHDFTGFTAS
                     GTSVENKVRTIFDAKIQFEASKNLLIFTFTGNGFLYKQVRNMVGTLLKIGNGRMPISQ
                     IKTILQAKNRDLAGPTAAGNGLYLKEIIYEDEECFSNFRK"
     gene            11205..11963
                     /locus_tag="VBC28_000168"
     CDS             11205..11963
                     /locus_tag="VBC28_000168"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEO6814178.1"
                     /translation="MKTRNVLAISGNDIFSGGGLHADLATYVVNKLHGFVAVTCLTAM
                     SDKGFEVIPIEASILKQQLESLKDVEFGSIKLGLLPNVETAQVVLEFVKSKQECPVVL
                     DPVLVCKENHDLEVSQLREQLIAFFPYADVITPNLVEAQLLTGLSIENLDQMKIAAEK
                     LYDMGAKHVVIKGGNRLNAEEATDLYYDGERFETYVFPVVDANNTGAGCTFASSIASQ
                     LAMGKNVEDAVKMSKGFVYQAIKASDKYGVVQHF"
     gene            11973..12437
                     /locus_tag="VBC28_000169"
     CDS             11973..12437
                     /locus_tag="VBC28_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEO6814179.1"
                     /translation="MRKEKTSQLVQLAIVTAISIVLGMFISIPTPTGFLTLLDAGIFF
                     AAFYFGKKEGAVVGALAGFLIDLLKGYPNWMFFSLLIHGTQGYLAGLPGRRRLLGLIS
                     ATLVMVLGYAIASGLMYGWGAVLPDIPGNIMQNMVGMVVGFALNKSLERVKK"
     gene            12434..13003
                     /locus_tag="VBC28_000170"
     CDS             12434..13003
                     /locus_tag="VBC28_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000599673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HEO6814180.1"
                     /translation="MILDYNKLKQETKAIVVDIIERSALKKGQIFVLGLSSSEVSGGL
                     IGKNSSSEIGEIIVEVILKELHSRGIYLAVQGCEHVNRALVVEAELAERQQLEVVNVV
                     PNLHAGGSGQVAAFKLMTSPVEVEEIVAHAGIDIGDTSIGMHIKRVQVPLIPISRELG
                     GAHVTALASRPKLIGGARAGYTSDPIRKF"
     gene            complement(13040..13882)
                     /locus_tag="VBC28_000171"
     CDS             complement(13040..13882)
                     /locus_tag="VBC28_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEO6814181.1"
                     /translation="MITLEKFIDHLNVEEVLFTFFTKLISILLLIIAFVIVRQVINYL
                     FEKTVNRSLAFSRQKVARQKTLAKLSHNVLNYTLYFFLFYWILSILGVPISSLLAGAG
                     IAGVAIGLGAQGFLSDVVNGFFILLENQFDVGDIINVGTVSGTVTNVGIRTTQIHDFD
                     GTLHFIPNRNITIVSNKSRSNMRAQIDIPLFVHTNLDQISDIVTKINEEYVSKHPAIV
                     GEPTVFGPTTNANGQFVYRINIFTQNGAQFDIYAEFYKLYQKAILEEGIDLPTYNFSN
                     NHSR"
     gene            14039..15322
                     /locus_tag="VBC28_000172"
     CDS             14039..15322
                     /locus_tag="VBC28_000172"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEO6814182.1"
                     /translation="MSTSFENKATNRGIITFTISQDEIKPALDQAFNKVKKDLNVPGF
                     RKGHMPRTVFNQKFGEEALYENALNLVLPKAYEAAVAELGLDVVAQPKIDVVSMEKGQ
                     DWKLTAEVVTKPEVKLGDYKDLSVEVDASKEVSDEEVDAKVERERNNLAELTVKDGEA
                     AQGDTVVIDFVGSVDGVEFDGGKGDNFSLELGSGQFIPGFEEQLVGSKAGQTVDVNVT
                     FPEDYQAEDLAGKDAKFVTTIHEVKTKEVPALDDELAKDIDDEVETLDELKAKYRKEL
                     ESAKEIAFDDAVEGAAIELAVANAEIVELPEEMVHDEVHRAMNEFMGNMQRQGISPEM
                     YFQLTGTTEEDLHKQYQADADKRVKTNLVIEAIAAAEGFEATDEEIEKEITDLASEYN
                     MEADQVRGLLSADMLKHDIAMKKAVDVITSSATVK"
     gene            15511..16086
                     /locus_tag="VBC28_000173"
     CDS             15511..16086
                     /locus_tag="VBC28_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HEO6814183.1"
                     /translation="MELEVFAGQEKSELSMIEVARAILEQRGRDNEMYFSDLVNDIQT
                     YLGKSDSAIRESLPFFYSDLNTDGSFIPLGENKWGLRSWYAIDEIDEEIITLEEDEDG
                     APKRKKKRVNAFMDGDEDAIDYNDDDPEDEDFTEETPSLEYDEENPDDEKSEVESYDS
                     EINEIIPDEDLDEDVEINEEDDEEEEEEEEV"
     gene            16359..17963
                     /locus_tag="VBC28_000174"
     CDS             16359..17963
                     /locus_tag="VBC28_000174"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEO6814184.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NIDPGTMSPYQHGEVYVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLKKE
                     RRGEYLGATVQVIPHVTDALKEKIKRAATTTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGSDNVMYIHTTLLPYLKAAGEMKTKPTQHSVKELRGLGIQPNMLVIRTEQPAG
                     QSIKNKLAQFCDVAPEAVIESLDVDHIYQIPLNMQAQNMDQIVCDHLKLETPAADMTE
                     WSAMVDKVMNLEKKVKIALVGKYVELPDAYLSVVEALKHSGYVNDVAIDLKWVNAAEV
                     TEDNIKELVGDADGIIVPGGFGQRGSEGKIEAIRYARENDVPMLGVCLGMQLTCVEFA
                     RNVLNLHGANSAELDPKTPFPIIDIMRDQIDIEDMGGTLRLGLYPCKLKSGSRAAAAY
                     NNQEVVQRRHRHRYEFNTKFREQFEAAGFVFSGVSPDNRLMEVVELPEKKFFVAAQYH
                     PELQSRPNHAEELYTAFVTAAVENMK"
     gene            18072..18998
                     /locus_tag="VBC28_000175"
     CDS             18072..18998
                     /locus_tag="VBC28_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEO6814185.1"
                     /translation="MKKIRLSKFIKMIVVILFLISVAASFYFFHVAQVRDDKSFISNG
                     QRKPGNSLYAYDKSFDKLLKQKIEMTNQNIKQVAWYVPAVKKTHKTAVVVHGFANSKE
                     NMKAYGWLFHKLGYNVLMPDNIAHGESHGQLIGYGWNDRENIIKWTEMIVDKNPSSQI
                     TLFGVSMGGATVMMASGEKLPSQVVNIIEDCGYSSVWDELKFQAKEMYGLPAFPLLYE
                     VSTISKIRAGFSYGQASSVEQLKKNNLPALFIHGDKDNFVPTSMVYDNYKATAGKKEL
                     YIVKGAKHAKSFETEPEKYEKRISSFLKKYEK"
     gene            19045..19130
                     /locus_tag="VBC28_000176"
     tRNA            19045..19130
                     /locus_tag="VBC28_000176"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19079..19081,aa:Leu,seq:aag)
     gene            19193..19639
                     /locus_tag="VBC28_000177"
     CDS             19193..19639
                     /locus_tag="VBC28_000177"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000049187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEO6814186.1"
                     /translation="MSKVRGFELVSQFSNKELLPKRETAHAAGYDLKVAKKTVIEPGE
                     ITLVPTGIKAYMQPGEVLYLYDRSSNPRKKGIVLINSVGVIDGDYYNNQVNEGHIFAQ
                     MQNITDQAVILEEGERIVQAVFAPFLLADDDQATGMRTGGFGSTGK"
     gene            19801..21165
                     /gene="radA"
                     /locus_tag="VBC28_000178"
     CDS             19801..21165
                     /gene="radA"
                     /locus_tag="VBC28_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEO6814187.1"
                     /translation="MAKKKSVFTCQECGYQSPKYLGRCPNCSAWSSFVEEVEVQEVKN
                     ARVSLNGEKSRPTKLKDVSSINYSRTKTDMDEFNRVLGGGVVPGSLVLIGGDPGIGKS
                     TLLLQVSTQLANKGTVLYVSGEESAEQIKLRSERLGDIDNEFYLYAETNMQSIRSEIE
                     KIKPDFLIIDSIQTIMSPEVSSVQGSVSQVREVTAELMQLAKTNNIATFIVGHVTKEG
                     TLAGPRMLEHMVDTVLYFEGERHHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNP
                     SQVFLEERLDGATGSAIVVTMEGTRPILAEVQALVTPTVFGNAKRTTTGLDFNRVSLI
                     MAVLEKRCGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKEKPTNPQESFIGE
                     IGLTGEIRRVTRIEQRINEASKLGFTKIYAPKNSLAGIEIPKGIDVIGVTTVSQVLKA
                     VFST"
     gene            21301..21798
                     /locus_tag="VBC28_000179"
     CDS             21301..21798
                     /locus_tag="VBC28_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEO6814188.1"
                     /translation="MTTYFDNFLKTNQAYADLHGTAHLPIKPKTKVAIVTCMDSRLHV
                     AQALGLALGDAHILRNAGGRVTDDVLRSLVISQQQLGTREIVVLHHTDCGAQTFTNEA
                     FAAQLQRDLGVDMHGHDFLPFNDIEESVREDVAKLHASPLIPDDVVISGAIYDVDTGR
                     MVEVK"
     gene            21952..23271
                     /locus_tag="VBC28_000180"
     CDS             21952..23271
                     /locus_tag="VBC28_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HEO6814189.1"
                     /translation="MKKYDVIVLGFGKAGKTLAAKLATQGKSVAMVEEDDKMYGGTCI
                     NIGCIPTKTLLVSASKNHDFQEAMTTRNEVTSRLRAKNFAMLDNKDTVDVYNAKARFI
                     SNKVVELTGGADKQELTADVIIINTGAKSVQLPIPGLADSQHVYDSTAIQELAHLPKR
                     LGIIGGGNIGLEFATLYSELGSKVTVIDSQSRIFAREEEELSEMAQDYLEEMGISFKL
                     SADIKSVQNEDEDVVISFEDEKLSFDAVLYATGRKPNTEGLALENTDIKLTERGAIAV
                     DEYCQTSVENIFAVGDVNGGPQFTYISLDDSRIVLNYLNCDKDYSLKNRGAVPTSTFT
                     NPPLATVGLDEKTAKEKGYQVKSNSLLVSAMPRAHVNNDLRGIFKVVVDTETNLILGA
                     RLFGAESHELINIITMAMDNKIPYTYFQKQIFTHPTMVENFNDLFNF"
     gene            23480..24934
                     /locus_tag="VBC28_000181"
     CDS             23480..24934
                     /locus_tag="VBC28_000181"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEO6814190.1"
                     /translation="MANKIRVRYAPSPTGLLHIGNARTALFNYLYARHHGGDFVIRIE
                     DTDRKRHVEDGERSQLENLRWLGMDWDESPETHENYRQSERLELYQRYIDQLLAEGKA
                     YKSYVTEEELAAERERQELAGETPRYINEFIGMSETEKEAYIAEREAAGIIPTVRLAV
                     NESGIYKWTDMVKGDIEFEGSNIGGDWVIQKKDGYPTYNFAVVIDDHDMQISHVIRGD
                     DHIANTPKQLMVYEALGWEAPQFGHMTLIINSETGKKLSKRDTNTLQFIEDYRKKGYM
                     SEAVFNFIALLGWNPGGEEEIFSREQLINLFDENRLSKSPAAFDQKKMDWMSNDYLKN
                     ADFESVFALCKPFLEEAGRLTDKAEKLVELYQPQLKSADEIVPLTDLFFADFPELTEA
                     EKEVMAAETVPTVLSAFKEKLVSLSDEEFTRDTIFPQIKAVQKETGIKGKNLFMPIRI
                     AVSGEMHGPELPDTIYLLGKEKSVQHIDNMLAKL"
     gene            complement(24974..25942)
                     /locus_tag="VBC28_000182"
     CDS             complement(24974..25942)
                     /locus_tag="VBC28_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEO6814191.1"
                     /translation="MKFGKKLGFLALLMSIVLILGACGKTGLGNSSGNSTKNVTKKSA
                     KDLKLGVSISTTNNPYFVAMKDGIDKYASNKKISIKVADAQDDAARQADDVQNFISQN
                     VDAILINPVDSKAIVTAIKSANNANIPVILMDRGSEGGKVLTTVASDNVAAGKMAADY
                     AVKKLGKKAKAFELSGVPGASATVDRGKGFHSVAKSKLDILSSQSANFDRAKALNTTQ
                     NMIQGHKDVQIIFAQNDEMALGAAQAVKSAGLQNVLIVGIDGQPDAHDAIKKGDISAT
                     IAQQPAKMGEIAIQAAIDYYKGKKVEKETISPIYLVTKDNVEKYNW"
     gene            complement(25995..26936)
                     /gene="rbsC"
                     /locus_tag="VBC28_000183"
     CDS             complement(25995..26936)
                     /gene="rbsC"
                     /locus_tag="VBC28_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771495.1"
                     /note="functions to transport ribose at high affinity;
                     forms a complex with RbsA2C2B; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HEO6814192.1"
                     /translation="MKNMMKYISELTTLIALAGLMVIITLINPNFLTTNNLLNLLLQV
                     TANGFIAFGMTFVILTGGIDLSVGSILALSSALTAGLIGHGVPVGIAIVLSVALGGIL
                     GMLNGLFISYGKLAPFIVTLATMTIFRGATLVYSNGNPITAGLSDSFLFQFLGQGYIV
                     GIPFPVILMFLTFIILYILLHKTAFGKSVYALGGNEKAAYISGIKLNKVKIIIYTISG
                     IMASISGLIITSRLSSAQPTAGASYEMDAIAAVVLGGTSLSGGKGRIIGTLIGALIIG
                     VLNNGLNIIGVSAFWQQVVKGIVILMAVLLDRFKVAK"
     gene            complement(26938..28416)
                     /locus_tag="VBC28_000184"
     CDS             complement(26938..28416)
                     /locus_tag="VBC28_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEO6814193.1"
                     /translation="MKIDMRNISKSFGTNKVLEKIDLELQSGQIHALMGENGAGKSTL
                     MNILTGLFPASTGTIYIDGEERTFSNPQEAEEFGISFIHQEMNTWPEMTVLENLFLGR
                     EIKTTFGLLNQKLMRQKALETFKRLGVTIPLDIPIGNLSVGQQQMIEIAKSLLNQLSI
                     LVMDEPTAALTDRETENLFRVIRGLKQEGVGVVYISHRMEEIFKITDFVTVMRDGVIV
                     DTKETSLTNSDELVKKMVGRKLEDYYPEKHSEIGPVAFEVSNLCGDNFEDVSFYVRKG
                     EILGFSGLMGAGRTEVMRTIFGIDKKKSGKVKIDDQEITITTPSQAIKQGIGFLTENR
                     KDEGLILDFNIKDNMTLPSTKDFSKHGFFDEKTSTTFVQQLINRLYIKSGRPDLEVGN
                     LSGGNQQKVVLAKWIGIAPKVLILDEPTRGVDVGAKREIYQLMNELADRGVPIVMVSS
                     DLPEILGVSDRIMVMHEGRISGELSRKEADQEKVMQLATGGK"
     gene            complement(28432..28830)
                     /gene="rbsD"
                     /locus_tag="VBC28_000185"
     CDS             complement(28432..28830)
                     /gene="rbsD"
                     /locus_tag="VBC28_000185"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HEO6814194.1"
                     /translation="MKKTGILNSHLAKLADDLGHTDRVCIGDLGLPVPNGIPKIDLSL
                     TSGIPSFQEVLDIYLENILVEKVILAEEIKEANPDQLSRLLAKLDNSVSIEYVSHNHL
                     KQMTQDVKAVIRTGENTPYSNIILQSGVII"
     gene            complement(28805..29716)
                     /gene="rbsK"
                     /locus_tag="VBC28_000186"
     CDS             complement(28805..29716)
                     /gene="rbsK"
                     /locus_tag="VBC28_000186"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HEO6814195.1"
                     /translation="MSNIVIIGSISMDLVMETNRIAKEGETVFGQRFSMVPGGKGANQ
                     AVAIGRLSQERDNITILGAIGEDSFGPILLDNLNKNHVTTDFVGTIPSSSGVAQITLY
                     NNDNRIIYCPGANGKVDTKKWSQEWSIIKEADLVVLQNEIPHQANMKIANFCKEHSIK
                     LLYNPAPSRETDIEMLDKVDYFTPNEHECQELFPNQKLEDILATYPEKLIVTLGTKGA
                     IYSDGKESHLIPALETKAVDTTGAGDTFNGAFGYAISKKFKIAKALRFATLAAHLSVQ
                     KFGAQGGMPTIKEMEDNQHYEKDWYIK"
     gene            complement(29709..30695)
                     /locus_tag="VBC28_000187"
     CDS             complement(29709..30695)
                     /locus_tag="VBC28_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017284954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEO6814196.1"
                     /translation="MSTIRQVAEKAGVSTSTVSRYISQNGYVSQKASQKIEQAIRELH
                     YVPNFLAQSLKTKKNQLVGLLLPDISNPFFPRLARGVEEFLKEQGYRVMLGNTNNKSH
                     LEEEYLNVLLQSNAAGIITTHDFTKNHPEIDIPVVVVDRVNQETQYGVFSDNKEGGKL
                     AAQAIWTAGATNILLIRGPLDKADNLNQRFQGSQNYLLNKGACFAIEDSASFDFAEIQ
                     IEAKTLLDHHPDIDSIIAPSDIHAIAYLHEILNRGKRIPEDVQIIGYDDILMSQFIYP
                     SLSTIHQSSYIMGQKAAELIFKITNQLPITNKRIKLPVHYVERETLRRKIDE"
     gene            30877..31965
                     /locus_tag="VBC28_000188"
     CDS             30877..31965
                     /locus_tag="VBC28_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEO6814197.1"
                     /translation="MSHAWKELTFYKKKYLLIELLIIVMMFMVVFLSGLANGLGRAVS
                     AAIENNPAQTYILNEGAEQVITSSVLTTKDQTDLNSLNLKDSTTLNIQRSSLTRQGHE
                     KKIDISYFAIDKDSFMAPTLSEGKQLTSYKKAIILNDSLKAEGIKLGDKVIDKSSSIS
                     LTVVGFVHNSMYGHGPVAFIDKDIYTEINKKINPQYQFLPQALVMKNDKSISHLPTQL
                     EAVSKKDVIQHIPGYSAEQSTLNMILWVLVVASAGILGVFFYIITLQKRHEFSVMKAI
                     GTKMSEIALFQLSQVIILALFGIIVGDGLAVALSYVLPAQMPFVINWQNIILVSFVFL
                     VIAMISSALSIVKVAKIDPVEVINGGGE"
     gene            31965..32651
                     /locus_tag="VBC28_000189"
     CDS             31965..32651
                     /locus_tag="VBC28_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6814198.1"
                     /translation="MSAILELKHISKHYPDGDELLSILDNLDLSVSAGEFVAILGPSG
                     SGKSTLLSIAGLLLGADQGSLYVNHENVTDLSQRQRTQLRREALGFIFQSHQLLPYLT
                     IQEQLQQEARFAKHYDKKTSLEEINKLLSDLGIEQCAHKYPNQLSGGQKQRAAIARAF
                     INHPKVILADEPTASLDEERGRQVTELIRQEVKSHNTAAIMVTHDERVLDLVDTVYRL
                     KDGKLVKENF"
     gene            32682..33353
                     /locus_tag="VBC28_000190"
     CDS             32682..33353
                     /locus_tag="VBC28_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO6814199.1"
                     /translation="MIKILVVEDNIVQQKIITTKLTQEGYQFITASNGQEALNCLDTE
                     EVQLIITDIMMPMMDGYQLIQELRSAAYNVPIIVMTAKSQMEDMTKGFGLGADDYMVK
                     PVQLQELALRIKALLRRANIVAQHQLIIGNTCLNEDELSLKYFEQEIIFPQKEFRVLF
                     HLLSYPNRIFTRLELLDSIWGMDTDLDERVVDACINKIRRKVEHLPDFKIETVRGVGY
                     RAKND"
     gene            33337..34416
                     /locus_tag="VBC28_000191"
     CDS             33337..34416
                     /locus_tag="VBC28_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEO6814200.1"
                     /translation="MQKMTKLRRFRFPLRFYFTLMFVLTMLFSVLASLLLVAAIVFTF
                     FQGVLTTHVLQVSALAVVFLSLVIASISMWYGSYHLTKPILDISHIVSNVADGDFEGH
                     IYRNSNRRKSYEYYNELDELSESINQMIVSLSHMDHMRKDFITNVSHELKTPIAAVAN
                     IVELLQDPELDEETQSELLGLVKTESLRLTRLCDTMLQMSRVDNQETIGELSSVRVDE
                     QIRQAMISLTERWQAKRINFQLDSKPYTVYSNSDLLMQVWINLLDNAIKYSEDIVDLS
                     VRMEETNNHYLRVIISDKGRGISQYDVQHIFDKFYQADQSHNQQGNGLGLAIVKRIIV
                     LCKGRISVSSQLEIGTEFCVELPLS"
     gene            34570..35760
                     /locus_tag="VBC28_000192"
     CDS             34570..35760
                     /locus_tag="VBC28_000192"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000514054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEO6814201.1"
                     /translation="MGKEKLILAYSGGLDTSVAIAWLKKDYDVIAICMDVGEGKDLDF
                     IHDKALTIGAIESYILDVKDEFAEHFVLPALQAHAMYEQKYPLVSALSRPIIAQKLVE
                     MAHQTGATTIAHGCTGKGNDQVRFEVAIAALDPELKVIAPVREWKWHREEEITFAKAN
                     GVPIPADLDNPYSIDQNLWGRANECGVLENPWNQAPEEAFGITKSPEEAPDCAEYIDI
                     TFQNGKPIAINNQEMTLADLILSLNEIAGKHGIGRIDHVENRLVGIKSREIYECPAAM
                     VLLAAHKEIEDLTLVREVSHFKPILENELSNLIYNALWFSPATKAIIAYVKETQKVVN
                     GTTKVKLYKGSAQVVARHSSNSLYDENLATYTAADSFDQDAAVGFIKLWGLPTQVNAQ
                     VNKG"
     gene            35779..37167
                     /gene="argH"
                     /locus_tag="VBC28_000193"
     CDS             35779..37167
                     /gene="argH"
                     /locus_tag="VBC28_000193"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEO6814202.1"
                     /translation="MTKNHKLWGGRFESSLEKWVEEFGASISFDQKLAPYDMKASMAH
                     VTMLGKTDIISQEEAGLIKDGLKILQDKYRAGQLTFSISNEDIHMNIESLLTAEIGEV
                     AGKLHTARSRNDQVATDMHLYLKDKLQEMMKKLLHLRTTLVNLAENHIYTVMPGYTHL
                     QHAQPISFGHHLMAYYNMFTRDTERLEFNMKHTNLSPLGAAALAGTTFPIDRHMTTRL
                     LDFEKPYSNSLDAVSDRDFIIEFLSNASILMMHLSRFCEEIINWCSYEYQFITLSDTF
                     STGSSIMPQKKNPDMAELIRGKTGRVYGNLFSLLTVMKSLPLAYNKDLQEDKEGMFDS
                     VETVSIAIEIMANMLETMTVNEHIMMTSTETDFSNATELADYLASKGVPFRKAHEIVG
                     KLVLECSKNGSYLQDIPLKYYQEISELIENDIYEILTAKTAVKRRNSLGGTGFDQVKK
                     QILLARKELKAE"
     gene            37308..38189
                     /locus_tag="VBC28_000194"
     CDS             37308..38189
                     /locus_tag="VBC28_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HEO6814203.1"
                     /translation="MAIVSAEKFVQAARDNGYAVGGFNTNNLEWTQAILRAAEAKKAP
                     VLIQTSMGAAKYMGGYKLCKQLIETLVESMGITVPVAIHLDHGHYDDALECIEVGYTS
                     IMFDGSHLPVEENLEKAREVVAKAHAKGISVEAEVGTIGGEEDGIVGKGELAPIEDAK
                     AMVETGIDFLAAGIGNIHGPYPANWEGLDLDHLKKLTEAVPGFPIVLHGGSGIPDDQI
                     QEAIKLGVAKVNVNTECQLAFCQATRDYAVEFNANEAEYMKKKLFDPRKFLKPGFDAI
                     TEAVEERIDVFGSANKA"
     gene            complement(38273..39190)
                     /locus_tag="VBC28_000195"
     CDS             complement(38273..39190)
                     /locus_tag="VBC28_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000197052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEO6814204.1"
                     /translation="MTRKIGIIGMGHVGSTVAHALIAQGIGDDYVFIDTNVDKVNADM
                     IDFQDALANATYHANLVLNDYDALSDADVVISALGDISLQNNKEANRFAELPFTSKEV
                     YDVSQKLKSSGFRGILIVVTNPVDAITQLYQEYTQFPKSRVIGTGTLLDTARMKRVLG
                     QRLQVDPRSIGGYNLGEHGNSQFVAWSQVTVKGQPAISLLKAEELEQIKEASLKGGHT
                     VFFGKKYTSYGIATAAVRIVLTVLNDSHELLPVSTFYESEGVYLGYPAIIGRVGIVER
                     ISLSLSPKEEQQLTDSAAFIRNYLKHINL"
     gene            39429..39617
                     /locus_tag="VBC28_000196"
     CDS             39429..39617
                     /locus_tag="VBC28_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEO6814205.1"
                     /translation="MAKVCYFTGRKTVSGNNRSHAMNQTKRTVKPNLQKVTVLIDGKP
                     KKVWVSARALKSGKVERV"
     gene            39749..40114
                     /locus_tag="VBC28_000197"
     CDS             39749..40114
                     /locus_tag="VBC28_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018365170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEO6814206.1"
                     /translation="MTVKINTRDGQIELSDDVIATVVGGSATEIFGVVGMASKSAIKD
                     NFQALLRKENYAKGVVVRSSEAGIVVDVYTVMSYGVKISEVSKNIQERVKFNLENQLG
                     LSADMVNVYVQSIKVVGED"
     gene            40114..41778
                     /locus_tag="VBC28_000198"
     CDS             40114..41778
                     /locus_tag="VBC28_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HEO6814207.1"
                     /translation="MSKITTSLFQEMVQAASTRLSKQAEYVNSLNVFPVPDGDTGTNM
                     GMTMENGAKEVSDKPATTVGEVGQILSKGVLMGARGNSGVITSQLFRGFGQSIKDKEE
                     LTGQDLAHAFQNGVEVAYKAVMKPVEGTILTVSRGAATAALKKAEETDDAVEVMRATL
                     KGAKRALAKTPDMLPVLKEVGVVDSGGQGLVFIYEGFLSALTGEYIASEDFKATPATM
                     TEMVNAEHHKAVVGHVATEDIKYGYCTEVMVGLKQGPTYVKEFNYEEFQGYLSNLGDS
                     LLVVNDDEIVKVHVHTEDPGLVMQEGLKYGSLVKVKVENMRNQHDAQMQKVEVEETVK
                     ETKEYGIIAVVAGDGLAEIFKSQGVDYIISGGQTMNPSTEDIVKAIEKVNARNVIILP
                     NNKNIFMAAQSAADVVDIPAAVVETRTVPQGFTSLLAFDPAKSLETNVADMTNSLSDV
                     ISGSVTLAVRDTTIDGLEIHENDILGMVDGKILVSTPDMEKALKDTFDKMIDEDSEIV
                     TIYVGEDGKQALAETLSEYLEETYEDVEVEIHQGDQPVYPYLMSVE"
     gene            41926..42810
                     /locus_tag="VBC28_000199"
     CDS             41926..42810
                     /locus_tag="VBC28_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEO6814208.1"
                     /translation="MSLIILTVILVLVIVLLITSLYVVKQQTVAIIERFGKYQKTATS
                     GIHIRVPLGIDKIAARVQLRLLQSEIIVETKTKDNVFVTLNIATQYRVNENNVTDAYY
                     KLIKPEAQIKSYIEDALRSSVPKLTLDELFEKKDEIALEVQHQVAEEMSTYGYIIVKT
                     LITKVEPDAEVKQSMNEINAAQRKRVAAQELANADKIKIVTAAEAEAEKDRLHGVGIA
                     QQRKAIVDGLADSIQELKDANVTLTEEQIMSILLTNQYLDTLNTFAINGNQTIFLPNN
                     PEGVEDIRTQVLSALKTR"
     gene            complement(42902..43018)
                     /locus_tag="VBC28_000200"
     CDS             complement(42902..43018)
                     /locus_tag="VBC28_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HEO6814209.1"
                     /translation="MAEKTDLSSAYRRLKSPNIKTRKRALKIIHEYKRYGKK"
     gene            complement(43386..43556)
                     /locus_tag="VBC28_000201"
     CDS             complement(43386..43556)
                     /locus_tag="VBC28_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6814210.1"
                     /translation="MKLKISVLTAASAVTLLLATLASLAKADTVSYPSRDAGFVYSRG
                     YAPKHFLSAYDK"
     gene            complement(43898..44638)
                     /locus_tag="VBC28_000202"
     CDS             complement(43898..44638)
                     /locus_tag="VBC28_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003063284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEO6814211.1"
                     /translation="MTQAILEIKHLKKSYGSNEVLKDISLSVNKGEVISIIGSSGSGK
                     STFLRSINLLEEPSGGEILYHGHNVLEKGYDLNNYREKLGMVFQSFNLFENLNILENA
                     IVAQTTVLKRERQEAEKIAKENLNAVGMTEQYWKAKPKQLSGGQKQRVAIARALSVNP
                     EAILFDEPTSALDPEMVGEVLKTMQDLAKSGLTMIIVTHEMEFAKEVSDRVIFMDKGI
                     IAEQGTPKQLFENPTQERTKEFLQRFLK"
     gene            complement(44648..46198)
                     /locus_tag="VBC28_000203"
     CDS             complement(44648..46198)
                     /locus_tag="VBC28_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEO6814212.1"
                     /translation="MKKILLSLFTALLITFGGMTSIQADEYLRVGMEAAYAPFNWTQN
                     DNTNGAVPIEGTDQYANGYDVQVAKKLAKKLNKKVVVVKTKWEGLVPALTSGKLDMII
                     AGMSPTEERKKEINFSKPYYISEPTLVVNAEGKYTNAKNISDFKNAKVTAQQGVYLYN
                     LIDQINGVKKEVAMGDFNQLRQAVESGVVDAYVSERPDATSAQTANPKLKMIELHQGF
                     KTSDADTNISVGMRKGDNRINQVNQVLESISRDKQIALMDKMIKEQPSVKKEKNGKPN
                     FFEQMATILKNNGSQFLRGTATTLLISMVGTIVGLFIGLLIGVFRTAPKSDNKLKAAL
                     QKLLGWLLNIYIEVFRGTPMIVQSMVIYYGTAQAFGVSLDRTLAAIFIVSINTGAYMS
                     EIVRGGIFSVDKGQFEAATALGFTHGQTMRKIVLPQVVRNILPATGNEFVINIKDTSV
                     LNVISVVELYFSGNTVATQTYQYFQTFTIIAIIYFILTFTVTRILRYIEKRFDSDNYT
                     TGANQLQV"
     gene            46334..48217
                     /locus_tag="VBC28_000204"
     CDS             46334..48217
                     /locus_tag="VBC28_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HEO6814213.1"
                     /translation="MKKCFLAICLALSFFMVSVQADEVDYNIPHYEGNLTIHNDNSAD
                     FTEKVTYQFDSSYNGQYVTLGTAGKLPDNFDINNKPQVEVSINGKVRKVSYQIEDLED
                     GYRLKVFNGGEAGDTVKVNVQWKLKNVLFMHKDVGELNWIPISDWDKTLEKVDFWIST
                     DKKVALSRLWGHLGYLKTPPKIRQNNNRYHLTAFNVNKRLEFHGYWDRSYFNLPTNSK
                     NNYKKKIEYQEKMIERHGFILSFLLRILLPSFFIIVTLFISIRVFLFRKKVNKYGQFP
                     KEHHLYEAPEDLSPLELTQSIYSMSFKNFQDEEKKTHLISQEQLIQSILLDLIDRKVL
                     NYDDNLLSLANLDRASDAEIDFIEFAFADSTSLKPDQLFSNYQFSYKETLRELKKQHK
                     ASDLQTQMRRRGSNALSRITRLTRLISKDNINSLRRKGISSPYRKMSSEESKELSRLK
                     RFSYLSPLISFVVIIYTLFLNYFTYFCIYLLLFGVILLLNKIIFMMTRKISNGYIVTE
                     DGASRVYQWTSFRNMLRDIKSFDRSELESIVLWNRILVYATLFGYADRVEKALRVNQI
                     DIPERFANIDSHQFAISVNQSSNHFSTITEDVSHASNFSVNSGGSSGGFSGGGGGGGG
                     GAF"
     gene            48263..49102
                     /locus_tag="VBC28_000205"
     CDS             48263..49102
                     /locus_tag="VBC28_000205"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEO6814214.1"
                     /translation="MLIIELLKALFLGVVEGVTEWLPVSSTGHLILVQEFMKLNQSKS
                     FVEMFNIVIQLGAIMAVIVIYFKRLNPFQPGKSAREIRLTWQLWLKVVIACIPSILIA
                     LPFDNWFEAHFNFMIPIAIALIFYGFVFIWVEKRNAHLKPQVTELASMSYKTAFLIGC
                     FQVLSIVPGTSRSGATILGAIIIGTSRSVAADFTFFLAIPTMFGYSGLKAVKYFLDGN
                     VLSLDQSLILLVASLTAFVVSLYVIRFLTDYVKRHDFTIFGKYRIVLGSLLILYWLVV
                     HLF"
     gene            49222..49977
                     /gene="mecA"
                     /locus_tag="VBC28_000206"
     CDS             49222..49977
                     /gene="mecA"
                     /locus_tag="VBC28_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000425372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HEO6814215.1"
                     /translation="MEMKQISETTLKITISMEDLEDRGMELKDFLIPQEKTEEFFYSV
                     MDELDLPENFKNSGMLSFRVTPKKDRIDVFVTKSELSKDLNLEELADLGDISKMSPED
                     FFKTLEQSMLEKGDTDAHAKLAEIENMMDKATQEVVEENVSEEQPEKEVETIGYVHYV
                     FDFDNIEAVVRFSQTIDFPIEASELYKNGKGYHMTILLDLENQPSYFANLMYARMLEH
                     ANVGTKTRAYLKEHSIQLIHDDAISKLQMIEMG"
     gene            49979..51139
                     /locus_tag="VBC28_000207"
     CDS             49979..51139
                     /locus_tag="VBC28_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000612108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HEO6814216.1"
                     /translation="MIPFTIEYIFVLIGAFLLSIILTPIIRVISLKVGAVDKPNARRI
                     NKVPMPSSGGLAIFLSFVVTTLFFMPMAASRHFIEVSYFHYILPVIIGGLVVTTTGFI
                     DDIFELRPRYKMLGIIIAAIIIWKFTHFRFDSFKIPIGGPLLEFGPILTFFLTVLWII
                     SITNAINLIDGLDGLVSGVSIISLATMAVVSYFFLPKIDFFLTLTIVILIASIVGFFP
                     YNYHPAIIYLGDAGALFIGFMIGVLSLQGLKNSTAVAVITPVIILGVPILDTAVAIVR
                     RKLSGKKISEADKMHLHHRLLSMGFTHRGAVLVVYGIAIIFSLIALLLNVSSRIGGIF
                     LLLALLLAMEIFIEGLNIWGENRTPLFNLLKFIGNSDYRQSVIAKYSDKHQK"
     gene            51304..52074
                     /gene="sufC"
                     /locus_tag="VBC28_000208"
     CDS             51304..52074
                     /gene="sufC"
                     /locus_tag="VBC28_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEO6814217.1"
                     /translation="MSVLEIKNLHVSIEDKEILKGLNLTLKTGEIAAIMGPNGTGKST
                     LSAAIMGNPNYEVTAGEILFDGEDILELEVDERARLGLFLAMQYPSEVPGITNAEFIR
                     AAMNAGKADDDKISIRQFITKLDEKMELLGMKEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPKFALLDEIDSGLDIDALKVVSKGVNEMRGEGFGAMIITHYQRLLNYITPDKVHV
                     MMDGKVVLSGGPELAVRLEKEGYAQIAEELGLEYKEEV"
     gene            52111..53373
                     /gene="sufD"
                     /locus_tag="VBC28_000209"
     CDS             52111..53373
                     /gene="sufD"
                     /locus_tag="VBC28_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEO6814218.1"
                     /translation="MSKEAILNFLQAKGEPTWLQELRLKAFEKIEELELPVIERVKFH
                     RWNLGDGTILENDYTANVPDFTELGNNPKLVQIGTQTVLEQVPMELIEKGVVFTDFYS
                     ALEEIPEVIERYFGKARPFEEDRLAAYHTAYFNSGAVLYIPDNVEITQPIEGLFYQDS
                     QSKVPFNKHILLIVGKNAKVSYLERFESIGDGTERTSANISVEVIAQAGSQIKFASID
                     RLGENVTTFISRRGRHSSDATIDWALGVMNEGNVVADFDSDLIGDGSHANLKVVAASS
                     GRQVQGIDTRVTNYGCNSVGHILQHGVILERGTLTFNGIGHIIKGAKGADAQQESRVL
                     MLSDKARSDANPILLIDENDVTAGHAASIGQVDPEDLYYLMSRGLNQKTAEQLVIRGF
                     LGTVIAEIPVKEVRDEMIAVIDTKLEKR"
     gene            53375..54607
                     /locus_tag="VBC28_000210"
     CDS             53375..54607
                     /locus_tag="VBC28_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEO6814219.1"
                     /translation="MTLLDSYKLKQDFPILNQLVNDEPLIYLDNAATTQKPNQVLEAL
                     RDYYQNDNANVHRGVHTLAERATAQYENAREKARQFLNAKLSKEILFTRGTTTGLNWV
                     AKFAESILERGDEVLISIMEHHSNIIPWQQACERTGAKLVYAYLKDGSLDLEDFYNKL
                     SSKTKFVSLAHISNVLGCVTPVKAIAERVHQVGAYMVVDGAQSAPHMAIDVQDLDCDF
                     FALSGHKMLGPTGIGVLYGKESILDKMPPVEFGGEMIDFVYEQSATWKELPWKFEAGT
                     PNIAGAIAFGEALDYLTDVGMDEIHQYEQSLVSYVLPKLQAIDGLTIYGPSDAESHVG
                     VIAFNLEGLHPHDVATAMDYEGVAVRAGHHCAQPLINHLGIHSAVRASFYFYNTKEDC
                     DKLVDAIQKTKEFFNGTL"
     gene            54594..55037
                     /locus_tag="VBC28_000211"
     CDS             54594..55037
                     /locus_tag="VBC28_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEO6814220.1"
                     /translation="MALSKLDNLYMAVVADHSKHPHHHGFLEGVEQVQLNNPTCGDVI
                     SLSVKFDGNIISDIAFAGNGCTISTASSSMMTDAVIGKTKEEALQLADVFSKMVQGDQ
                     NPKQEKLGDAEFLAGVSKFPQRIKCATLSWNALRKAIERDNQAEL"
     gene            55137..56555
                     /gene="sufB"
                     /locus_tag="VBC28_000212"
     CDS             55137..56555
                     /gene="sufB"
                     /locus_tag="VBC28_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017769002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEO6814221.1"
                     /translation="MSEINEKVEPQPIDLGEYKFGFHDDVKPIYSTGKGLNEAVIREL
                     SAAKGEPEWMLDFRLKSLETFNKMPMQTWGADLSDIDFDDIIYYQKASDKPARDWDDV
                     PEKIKETFERIGIPEAERAYLAGASAQYESEVVYHNMKEEYDKLGIVFTDTDSALKEY
                     PELFKKYFAKLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDIPLQTYFRINNENTGQ
                     FERTLIIVDEGASVHYVEGCTAPTYSSNSLHAAIVEIFALDGAYMRYTTIQNWSDNVY
                     NLVTKRATAKKDATVEWIDGNLGAKTTMKYPSVYLDGEGARGTMLSIAFANKGQHQDT
                     GAKMIHNAPHTSSSIVSKSIAKGGGKVDYRGQVTFNKDSKKSVSHIECDTILMDDISK
                     SDTIPFNEIHNSQVALEHEAKVSKISEEQLYYLMSRGLSEAEATEMIVMGFVEPFTKE
                     LPMEYAVELNRLISYEMEGSVG"
     gene            complement(56627..57814)
                     /locus_tag="VBC28_000213"
     CDS             complement(56627..57814)
                     /locus_tag="VBC28_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEO6814222.1"
                     /translation="MPKLIVSFLCILLSLTCVNSVQAEEHKDIMQITREAGYDVKDIN
                     KPKASIVIDNKGHILWEDNADLERDPASMSKMFTLYLLFEDLAKGKTSLNTTVTATET
                     DQAISKIYEISNNNIHAGVAYPIRELITMTAVPSSNVATIMIANHLSQNNPDAFIKRI
                     NETAKKLGMTKTHFYNPSGAVASAFNGLYSPKEYDNNATNVTTARDLSILTYHFLKKY
                     PDILNYTKYPEVKAMVGTPYEETFTTYNYSTPGAKFGLEGVDGLKTGSSPSAAFNALV
                     TAKRQNTRLITVVLGVGDWSDQDGEYYRHPFVNALVEKGFKDAKNISSKTPVLKAVKP
                     KKEVTKTKTKSIQEQPQTKEQWWTKTDQFIQSHFVSILIVLGTIAILCLLAGIVLLIK
                     RSR"
     gene            complement(57967..59202)
                     /locus_tag="VBC28_000214"
     CDS             complement(57967..59202)
                     /locus_tag="VBC28_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEO6814223.1"
                     /translation="MKKIITSILLLSCIFFMPTISAESFNASAKHALAVDLDSGKILY
                     EKDANKPAAIASLTKIMTVYMVYKEIDNGNLKWNTKVNISDYPYQLTRESDASNVPLE
                     KRRYTVKQLVDAAMISSANSAAIALAEHISGTESKFVDKMTAQLEKWGIHDSHLVNAS
                     GLNNSMLGNHIYPKSSQNDENKMSARDIAIVAYHLVNEYPSILKITSKSVAKFDKDIM
                     HSYNYMLPDMPVFRPGITGLKTGTTELAGQSFIATSTESGMRLLTVIMHADKADKDKY
                     ARFTATNSLLNYITNTYEPNLVLAKGAAYKGKEASVRDGKEQSVIAVAKNDLKVVQKK
                     NITKQNQLKINFKKELTAPITKKENLGKAYYVDLNKVGKGYLIKEPSVHLVAKDSIER
                     SFFLKVWWNHFVRYVNEKL"
     gene            59443..61098
                     /locus_tag="VBC28_000215"
     CDS             59443..61098
                     /locus_tag="VBC28_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEO6814224.1"
                     /translation="MAKQKNNWRRVGVGVLTLASVATLAACGSKSASQDSNGAINWAI
                     PTEINTLDLSKVTDTYSNLAIGNSSSNFLRLDKDGKTRPDLATKVDVSKDGLTYTATL
                     RKGLKWSDGSKLTAKDFVYSWQRLVDPKTASQYAYLAVEGHVLNADKINEGQEKDLNK
                     LGVKAEGDDKVVITLSSPSPQFIYYLAFTNFMPQKQEVVEKYGKDYATTSKNTVYSGP
                     YTVEGWNGSNGTFTLKKNKNYWDAKNVKTKEVRIQTVKKPDTAVQMYKRGELDAANIS
                     NTSAIYQANKNNKDVTDVLEATTAYMEYNTTGSVKGLDNVKIRRALNLATNRKGVVQA
                     AVDTGSKPAIAFAPTGLAKTPDGTDLAKYVAPGYEYNKTEAAKLFKEGLAESGLTKLK
                     LTITADADAPAAKNSVDYIKSTWEAALPGLTVEEKFVTFKQRLEDSRKQNFDIVVSLW
                     GGDYPEGSTFYGLFKSDSQNNDGKFANKDYDAAYNKAISEDAMKPAESAKDYKEAEKI
                     LFEQGAYNPLYFRSGKGLQNPKLKGVIRNTTGLSIDFTHAYKK"
     gene            61217..62131
                     /locus_tag="VBC28_000216"
     CDS             61217..62131
                     /locus_tag="VBC28_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6814225.1"
                     /translation="MIKYILKRVAILLVTLWVVITLSFFLMQILPGTPYNNPKLTEEM
                     IALLNKQYGLDKPVWQQYLTYLWNVLHGDFGTSYQSVNQPVSRMISLRLGVSVHLGVQ
                     ALVFGVLGGILVGAISARHKNDKVDGILSVIATLGISMPSFIIGILLLDYFGFKWNLL
                     PLSGWGTFSQTILPSLALGLPTLASVSRFFRSEMIETLNSDYVQLARSKGMTIRQVTR
                     KHAYRNSMIPILTLIGPLAAGLLTGSALIEQIFSIPGIGQQFVTSIPTKDYPVIMGTT
                     IVYAVMLMVAILITDVVISIVDPRVRLQ"
     gene            62141..63172
                     /locus_tag="VBC28_000217"
     CDS             62141..63172
                     /locus_tag="VBC28_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000764054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6814226.1"
                     /translation="MADKNRTFKLVGAGSSSTQEKIEKPALSFMQDAWRRLKKNKLAV
                     VSLYLLALLLTFSLASNLFVTQKDANGFDSKKVTTYRNLPPKLSSNLPFWNGSIKYAG
                     NTESTDAYKSQNVPEKVKYALGTDSLGRSVAKRIIVGIRISLLVAIAATFIDLIIGVT
                     YGLVSGFAGGRLDTLMQRIVEVISSIPNLVIVTMLGLVLGNGITAIIISIAFTGWTSM
                     SRQVRNLTLSYREREFVLAARSLGESPIKIAFKHILPNISGIIIVQIMMTIPSAIMYE
                     AVLSAINLGVKPPTASLGSLISDAQENLQYYPYQVILPALALVMISLAFILLGDGLRD
                     AFDPKSSDE"
     gene            63185..64231
                     /locus_tag="VBC28_000218"
     CDS             63185..64231
                     /locus_tag="VBC28_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6814227.1"
                     /translation="MEKETILSVNNLHVDFHTYAGEVKAIRDVNFELKKGETLAIVGE
                     SGSGKSVTTRTLIGLNAKNSEISGNVQFKGRNLVELSEEEWTKVRGNEISMIFQDPMT
                     SLDPTMKIGMQIAEPMMIHQKISKKDALKLALELMKDVGIPNAEEHINDYPHQWSGGM
                     RQRAVIAIALAADPEILIADEPTTALDVTIQAQILNLMKKIQAERDSSIVFITHDLGV
                     VAGMADRVAVMYAGKIVEFGTVDEVFYNPQHPYTWGLLNSMPTTDTESGSLESIPGTP
                     PDLLNPPKGDAFAARNEFALDIDHEEEPPYFKVSETHFAATWLLDERSPKVLPPLPIQ
                     KRWEKWNEIEGRKA"
     gene            64231..65163
                     /locus_tag="VBC28_000219"
     CDS             64231..65163
                     /locus_tag="VBC28_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO6814228.1"
                     /translation="MTENRKKLVEVKNVSLTFNKGKANEVRAIDNVSFDIYEGEVFGL
                     VGESGSGKTTVGRSILKLYDISDGEITFNGEVISHLKGKALHSFRKDAQMIFQDPQAS
                     LNGRMKIRDIVAEGLDIHKLAKSKSDRDSKVQALLDLVGLNKDHLTRYPHEFSGGQRQ
                     RIGIARALAVEPKFIIADEPISALDVSIQAQVVNLMQKLQREQGLTYLFIAHDLSMVK
                     YISDRIGVMHWGKLLEVGTSDDVYNNPIHPYTKSLLSAIPEPDPESERQRVHQPYNPA
                     IEQDGQERQMHEITPGHFVLSTPQEAEEYKKQIL"
BASE COUNT        21232 a        10464 c        13039 g        20524 t
ORIGIN      
        1 tattgatgtt gtattttttg ataaaaatat ttcttatgaa gaaacggtgg tattagaaca
       61 acaattaaag gataactatc cacagtacga ttgggaactt aaaaatgaat tttacatgaa
      121 cactcattct cctaataccc caaagtatac tagctctaaa gatgctattt caaaatttcc
      181 tgaaaagtgt actgcagtgg gagcaaggtt agatgataga aatcaactcg aactttacct
      241 cccttatggt gaagaggaaa tattaaattt cattgttagt cctacgccat attttgaaga
      301 ggatctatta cgttataatg tttatttgaa gcgagtggat aagaagaaat ggaataatat
      361 ttggcctcgt ttaacaatca taaaaaaatg ataacgttac tggagttggc ttaacagctt
      421 ttaaattttt agactgacaa atagtataga cctttaataa ggtcgtttat cgataatcat
      481 agtgtactgt gttataatcg ttatagcata aaaacacgtt gcagttggta gtctgcaagc
      541 gttcagtgaa cgtcagtaac cttccctcca ggtcgtccat tgcaatttaa tatttaaagg
      601 agggggctgt atgaaaatga cagtctattt tgatggtaat ttttggttgg gtttgattga
      661 gtacgatgat gatggtgatt acaaagtttt tcggtatttt tttggaaaag aacctaaaga
      721 tgatgatgtt tttaatttta taaatcataa acttaatgat ttgataaaaa aatatgagtt
      781 tgttaaaacg gatatttctc taaagcgtac gaatgaacac aaaaaaagtc ctaaaagaat
      841 gcaaagagaa attaatagag aaaaacgtaa gccagtagta tctacaaaag cgcaattggc
      901 gatgaaaact attcatatga gtataaaaaa cgaacgtcaa ttaagtcaga aatgtaagaa
      961 aaatgagtta agaaaacatc ggtaccaatt aaaacaagaa aaacgttacc aaaagaaaaa
     1021 aggacattga ttgtgtcatc atataaaaag cttgggagtg tcatctcaag ctttttatat
     1081 gttaactaat atgagtcact aaatagtctg ataatgctca attaatgtgt tttgggaaaa
     1141 aatcatttcc ttaaaggaca ccattgtcat gttgttatca ccttactttt tttatactta
     1201 gtatatgttt aagaaaggaa taagttgttt tgagagattt tgaggagttg tttgataaag
     1261 taaagccaat cgtaatgaag ctgagaagaa attattttgt tcaactttgg gaatatgatg
     1321 attggattca agaaggacgt attgtacttt ttaggttgct tgaagagcat ccgtatttat
     1381 tggataatga aagcaaacta ttcatttact ttaaaacaaa attttcaaat tatttaaacg
     1441 atgtattaag acaccaagat tgtcaaaagc gacaatttaa taaaatgcct tatgaagaaa
     1501 taagtgaagt tagtcactat gttaagagta aaggactagt tttagatgac tatattgctt
     1561 atcgtgatac tttaacaaaa gtggaagaaa ctttgtcaga tattgataaa gaaaaatttg
     1621 aaaaactgat tagtggtgaa cgttttgcgg ggaagaaaca attcatacga gatattcaac
     1681 cctttttcaa tgcttttaaa gcggattaat cgattttatt tctgcagtag atagctgcag
     1741 aattttttta tagggaaagt tcgctgatat attcctgtga aagcctttgt tttttgttat
     1801 aatataataa aatgcttgtg aggtgtcagt atgagtaaag ttcgtttata tatcgctcgt
     1861 catggtaaaa caatgtttaa tacaattggt cgcgctcagg ggtggagtga tactccttta
     1921 actacttttg gagaactcgg gattaaagag cttggtttgg gactaaaggc ttcaaatatt
     1981 agttttaaag aggctttttc tagtgatagt ggacgtactt tacaaactat ggaaattatt
     2041 ctcagggaag tgcaacaaga aaatattccc tatacgagag ataaacgaat ccgcgagtgg
     2101 tgctttggta gtttggatgg aggatatgat ggcgaccttt ttaatggcgt tctgcctcga
     2161 gtttcaaacg gagatatgag ccatttgaca cacgaagaaa ttgctaattt gatttgtcaa
     2221 gttgatacgg ctggctgggc agaaccgtgg gcaatattga gtaaccgtat tctgtcaggc
     2281 ttcactgcta tagctaaaaa aatagaggat attgggggag gtaatgcgat tgttgtcagt
     2341 catgggatga ctattgcgac ttttttatgg ttaatcgacc atagtacccc tcgtagttta
     2401 ggcttagata atggtagtgt ctcagtcgtt gattttgaag atggaacatt ttctattcaa
     2461 tctattgggg atatgtccta tcgagaaaaa ggaagagaaa ttttagaaaa aacattgcaa
     2521 tgaaaaaaaa taaaattatc cgattcagtt tagttggtgt tctacttgcg atactatgct
     2581 ttagtctttt tgctttattg aagcctaaca gtcaacaatc atcatctcaa aagttgagga
     2641 atgaggatat aaaaaagata tcctctcaaa aaagaaataa gaaattacaa ttaccagctg
     2701 tatcatcaaa agattggaac ttgattttgg tcaatcgtga ccataaacat gaagaattaa
     2761 gtccagatgt ggttcctgtt gaaaatattt atttggataa acgtattacg aagcaagcta
     2821 ctcagttttt agaggctgct agagcaattg attcacgaga acatttaatt tcgggttatc
     2881 gtagtgttgc ctatcaggag aagttgttca attcttatgt tactcaagag atgactagta
     2941 accctaattt gacgagggga caagcagaaa agttggtaaa aacttactct cagcctgcag
     3001 gtgctagtga acaccagact ggattagcga tggatatgag tactgtagat tctttgaatg
     3061 agagcgatcc tagagtagtc agtcagttga aaaagatagc tccacaatat ggttttgtct
     3121 tacggtttcc ggatggtaaa acagcagaaa caggggtagg ttatgaagat tggcattacc
     3181 gctatgttgg ggtagagtct gcaaaatata tggccaaaca tcatttaaca ttagaagaat
     3241 acataacttt attaaaggag aataaccaat gaggaaacgt ttttccttgc taaattttat
     3301 tgttgttact tttattttct ttttctttat tctttttccg cttttaaacc ataagggaaa
     3361 agtagatgct aattctaggc agagtgttac ctacaccaaa gaagaattta tacaaaaaat
     3421 tgtgccagat gcgcaagatc taggaaagtc gtacggtatt cgtccttcat ttattattgc
     3481 acaggcggct ttggattctg atttcggaga gaaaatatta gctaataagt atcataatct
     3541 gtttggtttg cttgcagaac caggaacgcc ctcaattacc ttaaatgata gtagtacagg
     3601 aaaaaaacag gaaaagcaat ttactcatta taaatcttgg aagtattcaa tgtatgatta
     3661 ccttgctcat ataaaatctg gagcgacagg caaaaaagat tcatatacta taatggtgtc
     3721 tgttaaaaat ccaaaaactt tagtgcaaaa attacaagat agtggttttg ataatgacaa
     3781 aaagtacgct aaaaaaatga cggaaatcat tgatttgtat gatttaacaa gatatgataa
     3841 gtgatattag cactctttga ataagagtgc taatttttga tgttttttcc ttgactatct
     3901 tttaaaaagg agtataataa aatcatagtt agcagtcgat gtgtaagagt gctaatttca
     3961 gaaagaccga gaggggtgat gtttttgtga ttacacaacg tcaaaatgat attttgaatt
     4021 taatcgttga gttatttaca caaactcatg aaccagtagg gtctaaagct ttacagagaa
     4081 cgattgattc tagtagcgcc actattcgta atgatatggc aaaactagag aagttggggt
     4141 tattggaaaa agcacacact tctagtggac ggatgccgag tccagctggt tttaagtatt
     4201 ttgtagagca ctcccttaga ctagatagta ttgatgagca agatatttat catgttataa
     4261 aagcttttga ctttgaagca ttcaaattag aagacatgct tcaaaaagct agtcatattt
     4321 tatcggagat gacgggctat acatccgtta ttttagatgt ggagccagct cgtcaacgtt
     4381 tgacagggtt tgatgttgtt cagcttagca accatgatgc tctagcagtc atgactttag
     4441 atgaatctaa gccagtgaca gtgcagtttg ctattccaag aaactttttg acacgtgact
     4501 tgatagcatt taaagctatc gtagaggagc gcttgctgga tggtagtgtc atggatattc
     4561 attataagtt gaggacagaa attcctcaga tcgttcagaa gtattttgta actacggata
     4621 atgttttaca actatttgat tatgtttttt cagagctgtt tctggaaacg gtatttgtag
     4681 ctggcaaggt aaattcttta acgtattcag atctatcaac gtatcagttt ttggataatg
     4741 agcagcaagt agcgatttct ttgaggcaaa gtctgaaaga gggtgagatg gcttctgtcc
     4801 aagtggcaga tagtcaagaa gccgcacttg cagatgtgtc agttttaact cacaaatttt
     4861 tgattcctta ccgtggattt ggactcctga gtttgattgg tcctattgat atggattatc
     4921 gtaggtctgt tagtttggta aacattatcg gaaaggtatt ggctgctaaa ttaggtgatt
     4981 attatcgata cctaaattct aaccattatg aagttcactg ataatggcag tattcaataa
     5041 gttatttaaa aggaggcact cagtgtcaga agaaattaaa aaagacgatt tacaggaaga
     5101 agtggaagcc actgaaactg aagagacagt tgaggaagtt attgaagaaa ttcctgaaaa
     5161 atcagagtta gaactcgcaa acgagcgtgc tgatgagttt gaaaataaat acttgcgagc
     5221 tcatgcagaa atgcagaata tacaacgtcg ctctagtgaa gaacgtcaac aacttcaacg
     5281 ctatcgttct caggatctgg caaaagcaat cttaccatcg cttgacaatc tagaacgtgc
     5341 cttggctgtt gaagggttga cagatgatgt taaaaaaggt ttagaaatga ctcgtgatag
     5401 ccttattcaa gcactaaagg aagaaggcgt agaagaagta gaagtcgact catttgacca
     5461 caactttcat atggcagttc agactctccc agccgatgat gaacatccag ccgatagcat
     5521 tgcagaagtc tttcaaaagg gatataaatt gcacgagcgt ctgctaagac cagcgatggt
     5581 agttgtctat aactaaggta aatgagtttt cgtttttgtc cgtaatgaca gtaaactaga
     5641 tagcaagtta gaagctattc agcttgctga ttaaactata gtgattgctt agaattggaa
     5701 gtaaaataat tcgagtgctt actaagataa attgaaataa aaagtaataa agtattataa
     5761 aataagaggt attaacatgt ctaaaattat tggtattgac ttaggtacaa caaactcagc
     5821 agtagcagtt cttgaaggga ctgaatcaaa aatcattgct aacccagaag gcaatcgtac
     5881 aactccttca gtagtatcat tcaaaaatgg tgaaattatc gtgggtgatg ctgcaaaacg
     5941 tcaagcggta acaaatccag atactgttat ctctatcaaa tcaaagatgg gaacttctga
     6001 aaaagtttct gcaaatggta aagaatatac tcctcaagaa atttcagcaa tgattcttca
     6061 ataccttaaa ggttatgctg aagactatct tggagaaaaa gtagaaaaag cagttattac
     6121 tgttccagct tacttcaacg atgcacaacg tcaggcaact aaagacgctg gtaaaattgc
     6181 aggtcttgaa gtagaacgta tcgttaacga accaacagca gccgcacttg cttatggtat
     6241 ggacaagact gacaaggatg aaaaaatctt agtttttgac cttggtggtg gtacatttga
     6301 cgtatcaatc cttgaattag gtgatggtgt cttcgacgtt cttgcaacag caggtgataa
     6361 caaacttggt ggtgacgact ttgaccagaa aattattgat ttcttggtag aagaattcaa
     6421 gaaagaaaat ggtattgatc tttctcaaga caaaatggct cttcaacgct tgaaagatgc
     6481 tgctgaaaaa gctaaaaaag acctttcagg tgtaactcaa actcaaattt cattaccgtt
     6541 catcactgct ggttctgctg gtcctcttca cttggagatg agcttatcac gtgctaaatt
     6601 tgacgatctc actcgtgacc ttgttgaacg tacgaaaact ccagttcgtc aagctctttc
     6661 agatgcaggc ttgtcattgt cagaaattga tgaagttatc ctcgttggtg gatcaacacg
     6721 tatcccagca gttgttgaag ctgtaaaagc tgaaactggt aaagaaccaa ataaatctgt
     6781 taaccctgat gaagtggttg ctatgggtgc tgctatccaa ggtggtgtta tcactgggga
     6841 tgtgaaagac gttgtacttc ttgacgtaac accattgtca cttggtatcg aaacaatggg
     6901 tggtgtcttc actaaattga tcgaccgcaa cacaacaatc ccaacatcta aatcacaagt
     6961 cttctcaaca gcagcagaca accaaccagc cgttgatatc catgttcttc aaggtgaacg
     7021 cccaatggca gcagataaca aaacactcgg tcgcttccaa ttgactgata tcccagctgc
     7081 acctcgtgga atcccacaaa ttgaagtaac atttgatatc gataaaaatg gtattgtatc
     7141 tgttaaagct aaagatctcg gtactcaaaa agaacaacac attgttatcc aatctaattc
     7201 aggattaact gatgaagaaa ttgataaaat gatgaaagat gctgaagcaa atgctgaagc
     7261 agatgcaaaa cgtaaagaag aagttgatct taaaaatgaa gttgaccaag ccatctttgc
     7321 aacagaaaaa actattaaag aaactgaagg caaaggcttt gatacagaac gtgatgcagc
     7381 gcaatcagca cttgatgagt tgaaaaaagc tcaagaatca ggtaaccttg acgacatgaa
     7441 agctaaactt gaagctctta acgaaaaagc acaagctctt gcagttaaac tttacgaaca
     7501 agcggctgca gcacaacaag cagctcaagg ggctgaaggt gcacaatcag ctgattcatc
     7561 aagcaagggt gatgatgttg tagatggcga attcactgag aaataattat taatattgtt
     7621 cagattcatt tgaatataag catgaaaact atactagcat agtaaagttc ttcgtgatag
     7681 gggttgctca ataatctaga taagtttcag attacataag ctaatttcgc tatcactaaa
     7741 taaaaacata ttaataataa ataggcgggg cgcctcgctc cgtctgtttt attaagtgtc
     7801 atatatatgt taactattta gagctgtaac tgggcaagaa taattgttaa tctcttcaag
     7861 tgtagtatat gaacaaaata taaaggatta gataatgaac aatacagaat tttatgatcg
     7921 tcttggcgtt tcaaaagatg cttctcagga cgaaataaaa aaagcttacc gtaggatgtc
     7981 taagaaatac catccagata ttaataagga gacgggagca gaggaaaaat ataaagaggt
     8041 tcaagaagct tatgaaacct tgagtgatac acaaaaacgt gctgcctatg atcaatatgg
     8101 cgctgctggg gcaaatggcg gttttggtgg cttcgatggt ggcggttttg gtggcttcga
     8161 tggtggtggc tttggtggtt ttgaagacat cttctcaagc ttttttggtg gaggagggat
     8221 gcgcaatcct aatgcaccac gtcaaggaga tgacctacaa tatcgtgtta atttaagttt
     8281 tgaagaagct atctttggag ctgaaaaaga agtatcttat aatcgggagt catcatgtca
     8341 tacttgttca ggaagtggag ctaagccagg tactagccct gtgacttgtc aaaagtgtca
     8401 tggttcgggt gtgattaatg ttgatacaca aacacctcta ggaacaatgc gtcgccaagt
     8461 gacttgtgat gtttgtcagg gtagtggtca agaaatcaaa gagaaatgtc caacatgtca
     8521 tggaactggt catgagaaga agacgcataa agtatcagtt aaaatacctg caggagttga
     8581 aactgggcaa caaattcgtt taacaggaca aggtgaagct ggcttcaatg gtggtcctta
     8641 tggtgatctc tttgttatta ttaacgttct cccaagccaa cagtttgagc gtaatggatc
     8701 aacaatttat tatactttga atattagctt tgtccaagct gctttgggtg atacaattga
     8761 tatacctact gtccatggag cggtagaaat gtcaattcct gctggtactc agacaggaaa
     8821 aacgtttcgt cttcgcggaa aaggtgcccc taaacttcga gggggtggac aaggtgatca
     8881 gcatgttact gttaacatcg taactcctac taaattgaat gatgctcaaa aagaggcatt
     8941 gcacgctttt gcagaggcaa gtggagataa gatggtacat ccgaagaaga aaggtttctt
     9001 tgataaagtc aaagatgctt tagatgttga ttaaaaaata aaagtctaga atcgaaagta
     9061 ttctagactt ttttgtattg ctagagggat tgcttgaacc taaattttac agtacaatga
     9121 atctaatatt gtagggggac aatttttatg gtaaccaaag ttgaagagat tcgctcatat
     9181 ttgatagctt ctatacaaaa tggtaagttg gctccaggag atcgcctacc atctataaga
     9241 cagttagcta atcaattttt ctgtaacaaa gatacagtgc aacgagtttt gatggaattg
     9301 cgttttgata attatatcta tgcaaagcct aggtcaggct attacgtctt tgattctcat
     9361 caagaggaag ttgaagaagg ggttagttta ccaaactctg agattgcaaa tatagcttat
     9421 gatgatttta gattgtgttt gaatgagacc cttattggta gggaagatta ccttttcaat
     9481 tattactatc gtcaagaagg tcttcttgat ttaagtaaag cagtggctaa attaatggaa
     9541 gaaacagggg tctatgttcc ccttgatgat attgttatta cggctggtac tcaacaggca
     9601 ttatttattt tgacacaggt tacctttcca aatcgaaaat ctcgagtttt aatagaagaa
     9661 ccgacctatc ctcgtatgat tgaactaatc aaaacacaaa atttacccta tgaaactatt
     9721 tctcgaggta ctcatggaat tgattttcag cgtttagagg agattttcca gacacaatca
     9781 attaagtttt tttatgttat acctcgcatg cataatcctt tgggaacatc ctataatccg
     9841 gtagagatga aaagattaat agagatggca gagaagtatg atgtttatat tgtggaagat
     9901 gactatatgt ctgattttgc aagtcagtca ccattacatt attatgatac tcacgggcgt
     9961 gttatttatc taaaatcttt ttcaaaggct attttccctg ctttaagatt agctgcgatt
    10021 tgtttaccac aagctttaaa atcaacattt atggcttaca agaagttgat ggattatgat
    10081 actaatctga ttttacaaaa agcattagcg ctttatattg aaaatggcct ttatgctaag
    10141 aatagtcaat atttgaaata tcgttatcag aaagaccttg caaattcaaa atctatttta
    10201 gctgatcacc ctaatctacc ctcatatagt ttacatcacg atagtgtatt atttgattgt
    10261 tcgaaactcg ataactttaa aatattacgg caatacggcg atactttgga aaattatttt
    10321 tgtcaaaaat cgcatcaatc tctcttacaa gtaaaaaatg attcctgctt aaagcagttc
    10381 ttgggatcgt tgtagtataa ttgtaggggg acagtttaaa atagataata tagttttcat
    10441 aaatgaaagg tggtaaaata aacccatgac aaggtataag gcacagattt cttatgatgg
    10501 ttcagctttt tcaggcttcc aaagacaacc aaattgtagg acagttcaag aagaaattga
    10561 gagaactctt aagcgcctaa atagcgggaa cgatgtgata atccatggtg ctggtcgcac
    10621 agatgttggt gttcatgctt atggacaagt tatccatttt gatttaccac aggcgcgtga
    10681 tgtagaaaaa ttacgctttg gtttagatac acagtgtcca gacgatattg atattgtcaa
    10741 agtagagcaa gtgtctgatg attttcattg tcgatatgat aaacacatta aaacttatga
    10801 gtttttagtg gatatcggta ggccgaaaaa tcctatgatg agaaactatg caacacatta
    10861 cccttatcct gtcattattg agcttatgca agaagctatt aaagatttag taggtaccca
    10921 tgactttaca ggctttactg cttcaggtac gagtgtagaa aataaagtac gtactatttt
    10981 tgatgcgaaa attcagtttg aagcgtctaa aaatctttta atttttacct ttacaggaaa
    11041 tggttttttg tataaacagg tgcgtaatat ggttggtacc ctgctaaaaa ttggtaatgg
    11101 acgcatgcct attagccaaa tcaaaacaat tttacaggct aaaaatcggg atttagcagg
    11161 tccaactgcg gcaggaaatg gtttatattt gaaggagatt atttatgaag acgaggaatg
    11221 ttttagcaat ttcaggaaat gatattttct ctggcggcgg cttgcatgca gatttagcaa
    11281 cttacgttgt taataaatta catggttttg tggcagtaac atgcttaaca gcaatgtcag
    11341 ataaagggtt tgaagtcatt cctatagagg catctatact aaagcagcaa ctagaaagtt
    11401 taaaagatgt agagtttggg tctattaaac taggattgct tcccaatgta gaaacagcac
    11461 aagttgtttt ggaatttgta aaatcaaaac aagaatgtcc agtagtctta gacccagtct
    11521 tagtttgtaa agaaaatcat gatttagaag ttagccaatt acgtgagcag ctaattgcgt
    11581 ttttcccata tgctgatgtg attactccta atttagtaga ggctcaatta ttgacgggtc
    11641 tttcgattga aaacttagat caaatgaaaa ttgctgctga gaaactttac gatatgggag
    11701 ctaaacatgt tgttatcaaa gggggcaacc gtttaaatgc tgaagaggct acagaccttt
    11761 actatgatgg tgaacgtttt gaaacctatg tttttcctgt agtagatgcc aataatacag
    11821 gtgctggatg tacatttgcg tcaagtattg ccagccaatt agctatgggt aaaaatgtag
    11881 aagatgcagt caaaatgtct aaaggtttcg tctatcaagc tattaaagca tcggataagt
    11941 acggagttgt ccaacatttt taggagattt taatgaggaa agagaaaact agtcaattgg
    12001 ttcaattggc aattgtaaca gctatatcta ttgtattggg gatgtttata agtattccga
    12061 caccaacagg ttttttgaca ttactagatg ctggaatatt ctttgctgcc ttttactttg
    12121 gcaaaaaaga aggggctgtg gtaggtgcct tagctggttt tttaattgat ttattgaaag
    12181 gttatcccaa ttggatgttc tttagtttat tgattcatgg gacgcaaggg tatttagcgg
    12241 gtttaccagg tagaagacgt cttttaggtc ttattagtgc aactcttgtt atggtattag
    12301 gttatgctat agctagtgga ttgatgtatg gttggggagc tgttctacca gatattcctg
    12361 gcaatattat gcaaaatatg gttggtatgg ttgttggttt tgctcttaat aagtctctag
    12421 aaagagtgaa gaaatgatac tagattataa taagttaaag caggaaacta aagctattgt
    12481 agttgatatt attgaacgta gcgctcttaa aaaaggtcaa atttttgtac taggtctatc
    12541 atctagtgag gtttctggtg gtttaattgg aaaaaattct agttctgaaa ttggtgaaat
    12601 tatcgttgaa gttatcctca aagagttaca ttcacgcggc atctatttag cagttcaagg
    12661 ttgtgaacat gttaataggg cgttggttgt tgaagcagaa ttggctgaac gtcaacagtt
    12721 ggaagtcgtt aatgttgttc ctaatctaca tgctggcggt agtggtcagg ttgcagcttt
    12781 taagttgatg acatcaccag ttgaggttga agaaattgtg gcacatgctg gaattgatat
    12841 tggagatact tctattggaa tgcatatcaa gagagtacaa gttcccttga ttccaattag
    12901 tcgtgagttg ggaggagcac acgttacagc actagctagt cgtcctaaat taattggcgg
    12961 tgctagagca ggatatacga gcgatccaat ccgtaaattt taaaataaac ctctgctctg
    13021 cagaggttta ttatttatct caacgagagt ggttattgga gaaattgtac gttggtaagt
    13081 caataccttc ttcaagaata gctttttgat aaagtttgta aaattcagca tagatgtcaa
    13141 actgagcacc gttttgcgtg aagatattaa tacgataaac aaattgacca ttagcattgg
    13201 ttgtaggacc aaaaacagta ggctctccaa cgatagcagg gtgttttgaa acatactctt
    13261 cattgatttt tgttacaata tcagaaatct gatcaaggtt tgtatgaaca aataaaggaa
    13321 tatcaatttg agcacgcata ttgctacgag acttattgct gacaattgta atattacgat
    13381 taggaatgaa gtgcaaggtt ccatcgaaat catgaatttg tgtggtgcgt atcccaacat
    13441 tagtaacagt gcctgataca gtaccaacat taataatatc accaacatca aattggtttt
    13501 ctagcagaat aaaaaagcca ttgacaacat ctgataagaa gccttgagca ccaagtccaa
    13561 tagcaacccc agcaatacca gccccagcta ataaactaga aattggaacg cccaatatac
    13621 ttaaaatcca atagaataga aagaaataaa gggtatagtt taaaacattg tgagaaagtt
    13681 tagctaatgt tttttgacga gctacttttt gtctagaaaa ggcgagtgag cgattaacag
    13741 tcttttcgaa aagataattg atgacttgcc taacgataac gaatgcaatg attaataata
    13801 agattgatat tagtttggtg aagaaagtaa ataaaacttc ctctacatta agatggtcta
    13861 taaatttttc taaagtaatc atagtaatag tctagtaaaa aaaaagccat agagcaagta
    13921 ttttaaaagc ttatcgttaa gcagtttaaa gcttcttttt tgcataaaaa ctttgtaaaa
    13981 cgttttaaat tatggtaaac taaactgtat ctaaataatt ttaaggagaa atgacttaat
    14041 gtctacatca tttgaaaata aagctacaaa ccgtggtatt atcacattta caattagtca
    14101 agatgagatc aagccagctc ttgatcaagc attcaacaaa gtaaaaaaag atttgaacgt
    14161 tccaggattc cgtaaaggac atatgcctcg tacagttttc aaccaaaaat ttggtgaaga
    14221 agcactctac gaaaatgcat tgaatttagt gttgccaaag gcttacgaag cagctgttgc
    14281 agaacttggt ttagacgttg ttgctcaacc aaaaattgat gttgtatcaa tggaaaaagg
    14341 tcaagattgg aagttgacag ctgaagttgt tacaaaacca gaagttaaac ttggagatta
    14401 caaagacctt tcagtagaag ttgatgcttc aaaagaagtt tcagatgaag aagttgatgc
    14461 taaagttgaa cgtgagcgta acaaccttgc agaattgact gttaaagatg gtgaagcagc
    14521 acaaggtgat acagttgtta ttgactttgt aggttcagtt gatggtgttg aatttgatgg
    14581 tggtaaagga gataacttct cacttgaact tggttcaggt caatttatcc caggatttga
    14641 agaacaatta gtaggttcta aagctggtca aactgtagac gttaatgtaa cattcccaga
    14701 agattatcaa gcagaagatc ttgctggtaa agatgctaaa tttgttacaa caattcatga
    14761 agtaaaaact aaagaagttc cagcacttga tgatgaactt gcaaaagata ttgatgatga
    14821 agttgaaaca cttgatgagt tgaaagcgaa atatcgcaaa gagctagaat cagcaaaaga
    14881 aattgcattt gatgatgcag ttgaaggtgc agcaatcgaa ttagctgttg ccaatgcaga
    14941 aattgttgaa ttaccagaag aaatggtcca tgatgaagta catcgtgcta tgaatgagtt
    15001 catgggtaat atgcaacgtc aaggaatttc accagaaatg tacttccaat taacaggtac
    15061 aactgaagaa gaccttcata agcaatacca agcagatgct gacaaacgcg ttaaaactaa
    15121 ccttgttatc gaagcaattg cagctgcaga aggctttgaa gcaacagatg aagaaatcga
    15181 aaaagaaatc acagatcttg cttcagaata caatatggaa gctgaccaag tacgcggtct
    15241 actttcagca gatatgctta aacatgatat tgcaatgaaa aaagcggttg acgttatcac
    15301 tagctcagct actgtaaaat aattgcttat taaattttga aaagattggg cttcccagtc
    15361 tttttatttg tgattattct tttgacgaat agctaaaaat agcgtaaaat agtaaatagc
    15421 gaaaatttga aaatatgact tatctatttt ctatctataa agaatattgc taaacaaaat
    15481 gatagtctta cacaattaag gagaataact ttggaattag aagtatttgc tgggcaagaa
    15541 aaaagtgaac tttcgatgat tgaagttgct cgcgctattt tagagcaacg tggtcgtgat
    15601 aacgaaatgt acttcagcga tttggtaaat gatattcaaa cctaccttgg aaaatctgac
    15661 tcagcaatcc gtgagtcatt gccatttttc tattcagatt tgaatacaga cggaagtttt
    15721 attccacttg gcgaaaacaa atggggactt cgttcatggt atgctatcga tgaaattgac
    15781 gaggaaatca tcactcttga agaagatgaa gatggcgctc caaaacgtaa gaagaaacgt
    15841 gtcaatgctt tcatggatgg tgatgaagat gctattgatt acaatgatga tgatccagaa
    15901 gatgaagatt tcacagaaga aacaccaagt ttagaatatg atgaagaaaa ccctgacgat
    15961 gaaaaatcag aggtagaatc gtatgattct gagattaatg aaatcattcc tgatgaagat
    16021 ttagatgagg atgtagaaat caacgaagaa gacgatgaag aggaagaaga ggaagaagag
    16081 gtttagacgg ggattttgta tttgacaaat agtcagatat agtttaaact attattcggg
    16141 cacctcttat gaggtcagat aaagctccct agtgataggg ggctattttg tttttgggaa
    16201 agatacagaa tggttccaga tagttttctg agtaggccct tgatcagata gtttctacta
    16261 gttttgacta gagaagctgg tattatttat caatcatgtg ttttgggttt tacccgtaaa
    16321 ctaactgtta gattaacatt taatttaagg agtttttaat gacaaagtat atttttgtta
    16381 caggtggtgt ggtctcttct attggtaagg ggattgttgc agcaagtctt gggcgcttac
    16441 taaaaaaccg tgggctaaag gtgacaattc aaaaatttga tccatatatt aatattgacc
    16501 cagggacaat gagcccttat caacatggag aagtttatgt tacagatgat ggtgcagaaa
    16561 ctgaccttga ccttggacac tacgagcgtt ttattgatat taacttaaac aaatattcaa
    16621 atgttacaac gggtaagatt tatagtgaag ttcttaaaaa agaacgccgt ggagaatacc
    16681 ttggtgcaac tgttcaagtt atcccacacg ttacagatgc tttgaaagaa aaaatcaaac
    16741 gtgctgcgac aactacagat tcagatgtta ttattactga agttggcggt actgttggag
    16801 atattgagag ccttccgttt ttagaagcac ttcgtcaaat gaaggcagat gttggttctg
    16861 ataatgttat gtacattcat acaacgttat taccatacct aaaggctgcc ggcgaaatga
    16921 aaacaaaacc gactcaacat tctgttaaag agttacgtgg cctaggtatc caacctaata
    16981 tgcttgttat ccgtacagag caaccagcag gtcaaagtat taagaataaa ttggcacaat
    17041 tttgtgatgt tgcaccagag gctgttatag agtcccttga cgttgaccat atttatcaaa
    17101 ttccacttaa catgcaagct caaaatatgg atcaaattgt ttgtgatcac ttgaagcttg
    17161 agacacctgc tgctgatatg acagaatggt cagcaatggt tgataaagta atgaaccttg
    17221 agaaaaaagt taaaattgca ttggttggta aatatgttga attacctgat gcctaccttt
    17281 cagttgtgga agcccttaag cactcagggt acgtcaacga tgttgctatt gatctaaaat
    17341 gggtaaatgc tgctgaagtg acagaagata atattaaaga acttgtcgga gatgcagatg
    17401 gtatcatcgt tcctggtgga tttggccaaa gaggtagtga gggtaaaatt gaagcaattc
    17461 gctatgcacg tgaaaatgat gttccaatgc ttggtgtttg tctcggaatg cagctaactt
    17521 gtgtcgaatt tgcgcgtaat gttttaaacc ttcatggagc taattcagca gagcttgacc
    17581 ctaaaacacc gttcccaatc attgatatca tgcgtgatca aatcgatatt gaagatatgg
    17641 gtggtacact tcgccttggt ctatatccat gtaaattgaa atcaggttca agagctgcgg
    17701 cagcttataa taatcaagaa gttgtccaac gtcgtcaccg tcaccgttat gaatttaata
    17761 caaaattccg tgagcaattt gaggctgctg gatttgtctt ctcaggggta tcaccggata
    17821 atcgtttgat ggaagttgtt gagttaccag agaaaaaatt ctttgttgca gcacaatacc
    17881 atccagaatt acaatcacgt cctaaccatg ctgaagagct ttacacagct tttgtaaccg
    17941 cagcagttga aaatatgaaa taactttatc gcctcccagt attaggaggc gattttattt
    18001 ttttagatag tttcactatc aaagtatcat tagctctttt ctttttctaa aaataaggta
    18061 taataggttg catgaaaaag attcgattat caaagtttat taaaatgatt gttgttattt
    18121 tgtttttaat tagtgtagca gctagttttt attttttcca cgttgcccaa gttcgagatg
    18181 ataaatcctt tatttcaaat ggtcaacgta agcctggaaa ctctttatat gcttatgata
    18241 aatcctttga taagctatta aagcaaaaaa tagaaatgac aaaccaaaat ataaagcaag
    18301 ttgcttggta tgttcctgct gttaagaaaa ctcataagac agctgttgtc gttcatggtt
    18361 ttgcgaatag caaagagaat atgaaggcat atggttggct gtttcataag ttaggataca
    18421 atgttcttat gcctgacaat attgcacatg gtgaaagtca tgggcagttg ataggctatg
    18481 gctggaacga ccgcgagaac attatcaaat ggacagaaat gatagttgat aagaatccat
    18541 caagccaaat tactttattt ggtgtttcaa tgggtggagc aacagtcatg atggctagtg
    18601 gtgaaaaatt acctagtcag gttgttaata tcattgaaga ttgcggttat tctagtgttt
    18661 gggatgaatt aaaatttcag gctaaagaga tgtatggttt accagccttc ccactcttat
    18721 atgaagtttc aacaatttct aaaatcagag caggtttttc gtatggacaa gcaagtagtg
    18781 tcgaacaatt gaaaaagaat aatttaccag ccctctttat tcatggtgat aaggataatt
    18841 ttgttccaac aagtatggtt tatgacaact ataaagctac agcaggtaag aaagagcttt
    18901 atattgtaaa aggggcaaaa catgcgaaat cttttgaaac agagccagaa aaatatgaga
    18961 aacgtatctc tagttttttg aaaaaatatg aaaaataata ttgacaacaa tatgataagt
    19021 tgatataatt tattatgttg ttaagcgggg atggcggaat tggcagacgc gcaagactaa
    19081 ggatcttgtg accgcttttg gtcgtgaggg ttcaagtccc tctctccgca gactagtcga
    19141 gttcatttgg aactcgactt tttgcatatt taagataaag atggagtatc acatgtcaaa
    19201 agtacgtggc tttgaattag ttagccaatt tagcaataaa gagttattac caaaacgaga
    19261 aacagctcac gcagctggct atgatttaaa agtagctaaa aaaacggtta ttgaacctgg
    19321 tgaaattaca ttggtcccga ccggtattaa agcatacatg caaccagggg aggtcctgta
    19381 cctttatgat cgttcatcga atccacgtaa aaaagggatt gtcctaataa attcagttgg
    19441 tgttattgat ggtgactact ataataacca agtcaatgaa ggacatattt ttgcacagat
    19501 gcaaaatatt actgatcagg ctgttatttt agaagaaggg gaacggattg ttcaggctgt
    19561 ttttgcccca ttcttattgg cagatgacga tcaggctaca ggaatgcgta caggtggttt
    19621 tggtagtact ggaaaataaa attattttac gaataatgtt agaaaaggct agacttactt
    19681 cttagccttt tttgatgaga gtaaacttat attttctcta acttggtgtg atataatgag
    19741 tgtactagtt atttggtacc aagctatcat attaatttag aagttataaa ggaggcctga
    19801 gtggctaaga aaaaatcagt cttcacctgt caagagtgtg gttaccaatc acctaagtat
    19861 ttgggacgtt gtccaaattg ctcagcctgg tcttcatttg tagaagaggt tgaagttcaa
    19921 gaagttaaaa atgcacgagt tagtttgaac ggtgaaaagt cccgaccgac gaagttaaaa
    19981 gatgtttcct caattaacta ttctagaact aagactgata tggatgaatt taaccgtgtt
    20041 ctaggaggcg gtgttgtacc aggaagtttg gttctgattg gaggagaccc tgggataggt
    20101 aaatctactt tattactaca agtatcaact cagctagcta ataaaggtac agtattatat
    20161 gtctcaggtg aggaatctgc agaacagatt aagttacgaa gtgagcgtct aggcgatatc
    20221 gataatgaat tttaccttta tgccgagact aatatgcaaa gtattcggtc ggagattgag
    20281 aagattaaac cagattttct tattattgat tctatacaaa ctattatgag tcccgaagta
    20341 tcaagtgtcc aaggctctgt tagccaagtt cgcgaagtga ccgctgaatt gatgcagtta
    20401 gctaaaacta ataatattgc tacttttatt gtaggtcatg tgactaagga gggaacatta
    20461 gcaggccctc gaatgctaga acatatggtg gatacggttc tttattttga aggggagcgt
    20521 catcacacgt tccgtatttt acgggcagtc aaaaaccgtt ttggttcgac caatgaaata
    20581 ggtatctttg aaatgcagtc gggtggtcta gttgaagttt tgaatccaag tcaagttttt
    20641 cttgaagaac gtttagacgg tgcaacagga tctgctattg ttgttactat ggaaggaaca
    20701 aggcctattt tagcagaggt tcaagcttta gtgactccga ctgtttttgg aaatgctaag
    20761 cgtacaacaa caggtcttga ctttaatcgt gtcagtctta ttatggcagt tctagaaaaa
    20821 cgttgtggtt tattgcttca aaatcaagat gcctatttaa aatcagcggg tggtgttaaa
    20881 ttggatgaac cagcaattga tttagctgtt gctgtagcta ttgcttccag ttacaaagaa
    20941 aaacccacaa atccacaaga atcatttatt ggagaaattg gactcactgg tgagattcgt
    21001 cgcgtgacac gtattgagca acgtattaat gaggctagta aattgggctt tactaaaatt
    21061 tacgcaccaa agaattctct agctgggatt gaaataccta aaggcattga tgttattggt
    21121 gtgacaactg ttagtcaggt cttaaaagca gtgttttcaa cataatgatt agattcggtt
    21181 cagccgaatc ttttttggtg gcgagaaaca tttcgtgaaa aataatgggc aaaatgataa
    21241 tataaaaggg ttggtattta gtaaatatcg aatgatgtta cactaacaaa ggagatctct
    21301 atgaccactt attttgataa ctttttaaaa accaatcaag cctatgctga cttacatgga
    21361 acagctcatc tacctatcaa gccaaagaca aaggttgcta ttgtaacctg tatggattct
    21421 cgtttacacg ttgctcaagc tctagggcta gctttaggag atgctcatat tttacgtaat
    21481 gctggtggac gagttacaga cgatgttcta cgttctttag ttatttcgca acaacaatta
    21541 ggaacacgag aaatcgttgt tctacaccat acagattgtg gagcacagac gtttactaac
    21601 gaagcttttg ctgctcaatt acagagagat ttgggtgttg atatgcatgg acatgatttt
    21661 cttcctttta atgatattga agaaagcgtt cgtgaagatg tggcaaaatt acatgcttct
    21721 ccattaatcc cagatgatgt tgttatttct ggtgctattt atgatgtaga tactggtcgt
    21781 atggtggaag ttaaatagaa aaaatcctgt ccttagcgat gggttttttt attttagaaa
    21841 actagattta aaggcaacag ttaggcttat catgccttat gctggaattc agacagagga
    21901 aacatctttt gctatactga aactatacta taaaagcgga ggaaatttaa aatgaaaaaa
    21961 tatgatgtca ttgttcttgg ttttggtaaa gcaggtaaaa cattagcagc aaaattagca
    22021 acacaaggta aaagcgttgc aatggtcgaa gaagacgata aaatgtacgg gggaacatgt
    22081 atcaacattg gatgtattcc gacaaaaacg cttttagtat cagctagcaa aaatcatgat
    22141 tttcaagaag ctatgactac tagaaatgaa gttacaagtc gtttacgtgc taaaaatttt
    22201 gctatgttgg ataataaaga cactgtggat gtttataatg ctaaagcccg ctttatatca
    22261 aataaagtgg tcgaattaac aggaggagct gacaaacaag aacttacagc tgatgttatt
    22321 attatcaata ctggtgctaa gtcagtacaa ttaccaatcc caggattagc tgattctcaa
    22381 catgtttatg attcaacggc aatccaagaa ttggctcatc ttcctaaacg gttaggtatc
    22441 attggaggcg gtaatattgg gcttgaattt gcgactttat attccgaatt agggagcaaa
    22501 gtgacagtta ttgatagtca atcccgtatc tttgcgcgtg aggaagagga attatcagag
    22561 atggctcaag attatttaga agagatgggg atatctttta aattatcagc cgatatcaaa
    22621 tctgtgcaaa atgaagatga agacgttgtt attagctttg aggatgaaaa attaagcttt
    22681 gatgcagtat tatatgcaac aggacgtaag ccaaatacag aaggtttagc acttgaaaat
    22741 acagatatta aattgacaga acgtggagct attgctgttg atgaatattg ccaaacttct
    22801 gttgagaata tttttgcagt tggtgatgtt aatggcggcc ctcaattcac atatatttct
    22861 ttagatgact cacgtattgt cttaaattat ttgaattgtg ataaggatta ttctctaaaa
    22921 aatcgaggag cagtaccgac ttcaaccttt acaaatcctc ctttagcaac ggtaggtcta
    22981 gatgaaaaga ccgctaagga aaaaggatat caagttaaat ctaatagcct tcttgtttct
    23041 gccatgccac gtgcacatgt taataatgac cttagaggta tttttaaagt tgtggtagat
    23101 actgagacaa atcttatttt gggagctcgc ttatttggtg cagaatccca tgaattaatt
    23161 aatatcatca caatggcaat ggataataaa atcccatata cttatttcca aaaacaaatt
    23221 ttcactcacc caacaatggt agagaatttt aatgatttgt ttaactttta agaatatttt
    23281 taaaaagtaa ggcaattttg ctttactttt tttatcgaaa attagtgtgg ctattattac
    23341 tgattatcat ttttagtgat ttagtggtat aatagggatg attggcaatc atctgtaaga
    23401 gtttgaatga tgttttgccc ccttgactaa ttcagttaaa ctgaaaaagt ttactatcag
    23461 aaaatatagg agtagaattt tggctaataa gattcgtgtg cgttatgcac caagtccaac
    23521 aggattgtta cacatcggaa atgcgcgtac tgcgctcttt aactaccttt atgctcgcca
    23581 tcatggtggg gattttgtta ttcgtattga agatactgac cgaaaacgcc acgtcgaaga
    23641 tggtgaacgc tctcaattgg agaacttacg ttggctaggt atggactggg atgaaagtcc
    23701 tgaaacacat gaaaattatc gtcaatcaga acgccttgag ctctaccaac gttatattga
    23761 tcagttgtta gcagaaggta aagcctacaa gtcctatgtg actgaggaag aattagcagc
    23821 agagcgtgaa cgccaagaat tggcaggcga aacacctcgc tatatcaacg aatttatcgg
    23881 aatgtcagaa actgaaaaag aagcttacat tgcagagcgt gaagcagcag gcatcattcc
    23941 gacagtacgc cttgctgtta atgaatctgg gatttataaa tggactgata tggtaaaagg
    24001 tgacattgag tttgaaggct ctaatatcgg cggagactgg gttattcaga agaaagatgg
    24061 ttatccaact tacaatttcg cagtagtcat tgatgatcat gacatgcaaa tttcgcatgt
    24121 tatccgtggc gatgaccata ttgcaaatac gccaaaacaa ttaatggttt atgaagcact
    24181 cggttgggaa gcgccacaat ttggtcacat gacattgatt ataaactcag agactggtaa
    24241 gaaattatca aaacgcgaca ccaatactct tcaatttatc gaagactatc gtaaaaaagg
    24301 ttatatgtcg gaagctgtct ttaatttcat tgcccttctt ggctggaatc ctggtggaga
    24361 agaagaaatc ttctcacgcg agcaattaat taatctattt gatgaaaatc gtcttagcaa
    24421 atcaccagca gcattcgatc agaagaaaat ggattggatg agtaatgatt acctaaaaaa
    24481 tgctgatttt gagagtgttt ttgcactttg caaaccattc ttagaagaag ctggtcgact
    24541 tacagacaaa gctgaaaaat tagttgagct ttatcaacct caattaaaat cagctgatga
    24601 gattgtacca ttaacagacc ttttctttgc agattttcca gaactaacag aagccgaaaa
    24661 agaagtaatg gcagctgaaa cagtcccaac agtcttatct gcttttaagg aaaagttagt
    24721 atcgctttct gacgaagaat tcactagaga tactattttc cctcaaatta aggctgttca
    24781 aaaagaaaca ggtattaaag gtaagaacct atttatgcct attcgtattg cggtttctgg
    24841 tgaaatgcat ggaccagagt taccagatac tatttattta ttaggtaaag aaaaatcagt
    24901 tcaacatatc gataatatgt tagcaaaatt ataatataaa aagtcgtgaa ttttcacgac
    24961 tttttgtgta cttttaccaa ttgtattttt caacattgtc tttggtaaca aggtaaattg
    25021 gagaaattgt ttctttctct acttttttgc ctttataata atcaattgct gcttgaattg
    25081 ctatttctcc catcttagct ggttgttgtg caatagtggc tgaaatatct ccttttttaa
    25141 tggcatcatg ggcatctggc tgaccatcaa ttcctacgat aagaacattt tgcaagccag
    25201 cagatttgac agcctgtgca gccccaagtg ccatttcatc attttgtgcg aaaataattt
    25261 ggacatcttt atgtccttga atcatatttt gtgtagtatt caaagctttg gcgcggtcaa
    25321 aattggcaga ttggcttgaa aggatgtcaa gtttagattt tgcaactgaa tgaaatcctt
    25381 taccacggtc tacagtagca gaggcacctg gtactccaga taattcaaag gcttttgctt
    25441 ttttgcctag ttttttaacg gcataatccg cagccatctt accagcagca acgttatctg
    25501 aagcgacagt agttaatact ttgcctccct cactaccacg gtccattaga ataacaggaa
    25561 tattagcgtt gtttgcagac ttaatagctg ttacaatagc ttttgagtca acgggattaa
    25621 tcaaaatagc atctacgttt tgactaataa agttttggac atcatctgct tgacgtgcgg
    25681 cgtcatcttg agcatcagct accttaatgc ttattttttt attgctagca tatttgtcaa
    25741 taccgtcttt catcgcaaca aagtagggat tgttagttgt tgaaatagat acacctagtt
    25801 tcaaatcttt agcagatttt ttggttacat tttttgtaga attcccagaa gaatttccaa
    25861 gtccagtttt accacatgca cctagaatga ggacaataga catcaataag gctagaaagc
    25921 caagtttttt tccaaatttc atttgaaact cctttagata aaatataatg aacttataga
    25981 aattagagta aagactattt tgctacttta aagcgatcaa gtaaaacagc cattaaaata
    26041 acgatacctt tcacaacttg ttgccaaaat gcagagacac caatgatgtt taatccattg
    26101 ttcaggacac caataataag agcaccaatc aatgttccaa tgatacgtcc tttcccacca
    26161 gataaagacg ttccacctaa gacaacggcg gcaatagcat ccatctcata acttgctcca
    26221 gctgttggtt gtgctgagct aaggcgtgat gtgataatta acccagaaat ggaagccatg
    26281 atacctgaga tagtgtagat gataatttta actttgttta acttaatacc cgagatatac
    26341 gctgcttttt cattacctcc taaagcgtaa acagatttcc cgaaggctgt tttatgtaac
    26401 aaaatataca aaataataaa agtaagaaac atgagaatga ctgggaatgg gataccaaca
    26461 atatagcctt gtcccaaaaa ttggaataga aaactatcgg ataaaccagc ggtaattggg
    26521 ttgccgtttg aataaactag cgttgctcct ctaaagatag tcattgttgc cagtgtaacg
    26581 ataaatggtg ctaattttcc gtaagaaatg aagagtccgt ttagcattcc caagatgcca
    26641 cctaaggcaa cagataaaac gatagcaata ccaacgggta ctccgtggcc gattaatcca
    26701 gctgttaatg cactagatag ggctaatatg gacccgactg ataaatcaat accgccagtc
    26761 aaaataacga aggtcatccc gaaagcgata aaaccatttg cagttacttg taataataaa
    26821 ttgagtagat tattggttgt gaggaaatta ggattaatga gtgtgataat aaccattaaa
    26881 ccagctaacg caatcagggt tgttaactct gatatatatt tcatcatatt tttcacttta
    26941 cttacctcct gtggctagtt gcataacttt ttcttggtca gcttcttttc gactgagttc
    27001 tccactgatt cgaccttcgt gcattaccat gatgcggtca ctaacaccta gaatttcagg
    27061 taagtctgat gataccatga caattggaac acctctgtct gctagttcat tcattaattg
    27121 gtaaatctct cgtttagcac cgacatcaac accacgcgtt ggctcatcaa gaattaagac
    27181 tttaggagca ataccaatcc atttagcgag aacgaccttt tgttggttac ctccagaaag
    27241 atttccaacc tctaaatctg gtctccctga tttaatgtag agacgattaa tcaattgttg
    27301 cacaaatgtt gtgcttgttt tttcatcaaa gaaaccatgt ttgctaaaat ctttagtgct
    27361 agggagagtc atattgtcct tgatattgaa gtctaggatg agaccttcat cttttctatt
    27421 ttcggtcaaa aaaccaatgc cctgcttgat agcttggctt ggggtagtaa tagtgatttc
    27481 ttggtcatca attttcacct taccagactt ctttttatca attccaaaga tagttcgcat
    27541 aacttcagta cgcccagctc ccattaatcc agaaaatcca agaatctcgc ctttacggac
    27601 ataaaaggaa acgtcctcga aattatctcc acagaggtta ctaacttcaa aagcaacagg
    27661 cccaatttca gaatgttttt caggatagta atcttccaat tttcggccaa ccattttttt
    27721 aaccaattcg tcagaatttg tgagtgatgt ttcttttgta tctacaatga caccatctct
    27781 catgacggtt acgaagtcag tgattttaaa aatttcctcc attcgatggg agatatagac
    27841 aacgcccact ccttcttgtt tcaaacctcg gataactcgg aaaagatttt cagtttcacg
    27901 atctgttaaa gcagcagtcg gttcatccat taccaaaata gagagttggt ttaggagact
    27961 tttggcgatt tcaatcattt gttgttgtcc aacagacaag ttaccaatcg ggatgtctag
    28021 gggaatagtc acacctaatc ttttaaaagt ttcaagtgct ttttgtctca ttaatttttg
    28081 attgagtaag ccaaaagttg tcttgatttc acgtccaaga aagagatttt ccaagacagt
    28141 catttccggc caagtattca tctcttggtg aataaaacta ataccaaatt cctctgcttc
    28201 ttgagggttt gaaaaagttc gttcctcgcc atcaatataa atagttcctg tacttgctgg
    28261 aaaaagtcct gttagaatgt tcattaaggt tgatttaccc gcaccatttt cacccataag
    28321 cgcgtgtatt tgaccagatt gtaattccaa atcaatcttt tctagaactt tattagtacc
    28381 gaaggatttg gagatattcc tcatgtcaat tttcatggac taaactcctt tctagatgat
    28441 aacaccagat tgtagaataa tatttgaata cggtgtattt tcacctgtac gaataactgc
    28501 tttgacatct tgggtcattt gttttaaatg attgtgactg acgtactcta tgctaacaga
    28561 gttatcaagt ttagctaata accttgataa ttgatccgga tttgcttcct tgatttcttc
    28621 tgctaaaatg actttttcaa ctaagatatt ctctaaatag atatcaagta cctcttggaa
    28681 actaggtatt ccagaggtca aagacaaatc aatctttgga atgccatttg gaacaggtag
    28741 acccagatct ccaatacaaa ctcgatcagt atgcccaagg tcgtctgcta atttagcgag
    28801 atgactattt aatataccag tctttttcat aatgttggtt gtcctccatt tctttaattg
    28861 taggcatgcc accctgtgca ccaaattttt gcacggataa gtgagcagct agagttgcaa
    28921 aacgtaaggc cttagcaatc ttaaattttt tcgagatagc ataaccgaat gcaccgttaa
    28981 aagtatcacc tgcaccagtt gtatcaacag ctttagtttc taaagcagga attaaatgac
    29041 tttcttttcc atcagagtag atggctcctt ttgtgccgag tgttacgatt agtttttcag
    29101 gatatgtggc taatatatcc tctagttttt ggtttgggaa cagctcttga cattcgtgtt
    29161 catttggtgt gaaataatcg accttatcga gcatctcaat atcagtctct ctagatggtg
    29221 caggattata gagtaattta atactatgtt ctttacaaaa attagcaatt ttcatatttg
    29281 cttgatgagg aatttcattt tgtaatacaa ctaaatcagc ttctttaatt attgaccact
    29341 cctgagacca ttttttagta tcaacttttc cgttggctcc aggacagtaa atgattcgat
    29401 tatcattatt ataaagagtg atttgggcaa cacctgaaga agatggtatc gttcccacaa
    29461 aatccgtagt aacatgattt ttgtttaaat tatccaaaag aatgggacca aaagaatcct
    29521 cgccaatggc accaagaata gtaatattat ctcgctcttg gctaagacgc ccaatagcga
    29581 cagcctgatt agcaccttta cctcctggaa ccattgaaaa acgttggcca aatacggttt
    29641 ctccctcttt ggcgatacga ttggtttcca taactaagtc catggaaata ctaccgataa
    29701 tgacaatgtt actcatctat tttcctcctt agggtttcac gttcgacata atgaacgggt
    29761 aatttaatac gcttattggt aataggtaat tgattagtaa ttttgaaaat taattcagct
    29821 gctttttgtc ccataatata tgatgattgg tgaatagttg acaacgaagg gtagataaat
    29881 tggctcatta aaatatcatc ataaccaata atttgtacat cttcagggat tcgcttccct
    29941 cgattaagaa tctcgtgtag gtaggcaata gcatgtatat cagatggagc gataatgcta
    30001 tcaatgtctg gatggtggtc aagtaaagtt ttcgcttcaa tttgaatctc tgcaaaatca
    30061 aaactggcac tatcttcgat agcaaaacaa gcccctttgt ttaataggta attttggctt
    30121 ccttgaaacc tttgattaag gttatcagct ttatctagcg gaccacgaat gagtaatata
    30181 ttggtagcac ctgctgtcca aatagcttga gctgctaatt ttcctccctc tttattatca
    30241 gagaaaacgc cgtactgtgt ttcttgatta acgcggtcaa caactacaac tggaatatct
    30301 atttcaggat gatttttggt gaaatcgtgt gttgtaataa tccctgcagc attactttgt
    30361 aatagcacat ttaagtactc ttcttctaaa tggctcttgt tatttgtgtt gccaagcatg
    30421 acacgataac cctgttcttt tagaaattct tcaactcctc tagcaagtct tggaaagaaa
    30481 gggtttgaaa tatcgggaag tagtaatcca acaagttgat tttttttggt tttcagagat
    30541 tgtgcaagga aatttggaac ataatggagt tcgcggatag cttgctcgat tttttgactt
    30601 gctttttgac taacgtaacc attttgggat atataccttg agacagtaga tgttgatacg
    30661 ccagctttct cggcgacttg tctaatcgta ctcatagaaa ctcctttctt atattgtgga
    30721 aacggtttca caattagaat tgtaatcgct tactaaaaaa ttgtcaagct attttttaaa
    30781 taatttaatg ataaaatatc attattttta gaggtcatat tcaagtcata aagctgtgat
    30841 actatgttca catcaaaaga tcaaaggaga aaaaccatgt cacatgcttg gaaagaatta
    30901 accttttata agaaaaaata cctcctgatt gaattgctaa tcattgttat gatgttcatg
    30961 gtagtttttt tatcaggttt agcaaacggt ttaggtagag ctgttagtgc tgctattgaa
    31021 aataacccag ctcaaaccta tattttgaat gaaggtgctg aacaagtgat tacatcatct
    31081 gtattgacaa ctaaagatca gacagattta aacagtttga atttaaaaga tagtaccact
    31141 ttaaatattc aacgttcgag tttaactcga caaggacacg aaaagaaaat tgatatttct
    31201 tattttgcta ttgataaaga ttcttttatg gcacctacac tttctgaggg aaaacaactg
    31261 acaagttaca aaaaagctat tattttaaat gatagtttaa aagctgaagg gataaagtta
    31321 ggtgataaag tgattgataa gtcatcatct atatcactaa cagtcgttgg atttgtccat
    31381 aatagtatgt atggtcacgg acctgtggca tttattgata aagacattta taccgaaatt
    31441 aataaaaaga ttaatcctca ataccagttc ttacctcagg cattagttat gaaaaatgat
    31501 aaaagtatat ctcatttacc aactcaatta gaagctgtta gtaaaaaaga tgtcattcaa
    31561 catattcctg gttatagtgc cgaacaatcc actttgaata tgattttatg ggtccttgtc
    31621 gtagcttcag ctggtatttt aggtgtcttt ttctacatca tcacacttca aaaacgtcat
    31681 gaatttagcg tcatgaaagc tattgggaca aaaatgtcag agattgccct atttcaattg
    31741 agtcaagtca ttatattggc actgtttggt atcattgttg gagatggatt ggcggttgct
    31801 ctctcttatg tattaccagc tcaaatgcca tttgttatta attggcaaaa tattattcta
    31861 gtatcgtttg tatttttagt cattgcaatg attagtagtg ctttgtcaat tgtaaaagtt
    31921 gctaaaatcg acccagtcga agttattaat gggggaggag aataatgtca gcaatcttag
    31981 aattaaaaca catctcaaag cactatccag atggagatga gcttttatca atattagata
    32041 atttagattt atcagtttct gcaggtgagt ttgtagctat actgggaccc tcaggttcag
    32101 gtaaaagcac acttttatcc attgcagggt tacttcttgg cgcagatcaa ggtagtttgt
    32161 atgtcaatca tgagaacgtt acggatttgt ctcaaagaca aagaacacaa ttgcgccgtg
    32221 aagcactagg ttttatattt caaagtcatc aactgcttcc ttatttaaca atccaagaac
    32281 agttgcaaca agaggctcga tttgcaaaac actatgataa gaaaacaagc ttggaagaaa
    32341 ttaataaatt attatcagat ttaggaatag agcagtgtgc acataaatac cctaaccaat
    32401 tatcgggagg acaaaagcaa cgcgcagcaa ttgctagagc atttattaat catcctaagg
    32461 ttatcttggc agatgagcca acagctagcc ttgatgaaga acgtgggcgt caagttacag
    32521 aacttattcg tcaggaagtt aaatcacata atactgcagc tattatggtg acacacgatg
    32581 agcgcgttct agatttagta gataccgttt atcgtttaaa ggacgggaaa ttagttaaag
    32641 aaaacttcta acttgtgtta caataagcta gaggtgattt aatgattaaa atattagttg
    32701 tagaggataa cattgttcaa caaaaaatca ttaccacaaa attaacacaa gagggctacc
    32761 aatttatcac tgcttcaaat ggacaagaag ctctaaattg tctagataca gaagaagtac
    32821 aattgattat aactgatatc atgatgccca tgatggatgg ttatcaatta attcaagaac
    32881 tcaggtcagc tgcttataat gttccaatca ttgttatgac agcaaaatca caaatggaag
    32941 atatgacaaa agggtttggc cttggtgcag atgattatat ggttaagcct gtgcagttac
    33001 aagaattagc tttacgtatt aaggcactct tgaggagagc taatattgta gctcaacatc
    33061 agctaattat tgggaatact tgtcttaatg aagatgaatt aagcttgaaa tattttgagc
    33121 aagaaattat atttcctcag aaagaattta gggttctttt ccacttactt agctatccta
    33181 ataggatttt cacacgttta gaactattgg atagtatttg gggaatggat actgatttag
    33241 atgagcgggt ggtagatgct tgcatcaata aaattagacg aaaagtagaa cacttaccag
    33301 attttaagat tgagacggtg agaggggtag ggtatcgtgc aaaaaatgac taagctaagg
    33361 cgatttcggt ttccgttaag attttatttc acattaatgt ttgtgttaac gatgcttttc
    33421 tcagtcctag cttctctttt actagttgca gcaatagttt ttactttttt tcaaggagtc
    33481 ttaactactc atgtattaca agttagtgct ctcgctgttg tttttttaag ccttgtgata
    33541 gctagtatta gtatgtggta cggttcttat catttaacaa aaccaatttt agatattagc
    33601 catattgtgt ccaatgttgc agacggtgac tttgaagggc atatttatcg aaacagtaat
    33661 cgtcgtaaat cctatgagta ttataatgaa ttggatgagc tatctgaaag tattaatcag
    33721 atgattgttt ctttatctca tatggatcat atgcgaaaag actttatcac taatgtttct
    33781 catgagctaa agactccgat tgctgcagta gctaatattg tagaactatt acaagatcca
    33841 gaattagatg aagaaacaca aagtgagtta ttaggtcttg ttaaaacaga gagcctaagg
    33901 ttaacaagat tatgtgatac gatgttgcaa atgtcgcgcg tggataatca ggagactatt
    33961 ggggagctat cctctgttcg tgtagatgaa caaattcgtc aagcaatgat tagtttaaca
    34021 gaacgttggc aagctaaaag gataaatttt caactggatt caaaaccata tactgtttac
    34081 tcaaactctg acttgctgat gcaagtttgg ataaaccttt tagataatgc aataaaatat
    34141 tccgaggata ttgtagactt aagtgtacga atggaagaaa ctaataatca ttatctgaga
    34201 gttattattt ctgataaagg taggggaatt tctcaatatg atgttcagca tatatttgat
    34261 aaattttacc aagcggatca gtcgcataat caacaaggaa atggattagg actagcaata
    34321 gttaaacgta taattgttct ttgtaaggga cgtattagtg tctccagtca gttggaaatt
    34381 ggaacagaat tttgtgtaga actaccttta tcataattat ttaaaacaat aactgccaat
    34441 tggcagttat tgttttattt gtttagaaat aaatatacta aaaatagaca aaaactataa
    34501 aaatatttga caataaatat agcaagtgta taatattaca aaacatagga atttggaagg
    34561 ggatttttta tgggaaaaga aaaattaatt ttagcttatt ctggtggttt agatacctct
    34621 gttgctatag cttggttaaa aaaagattat gacgttattg ctatctgtat ggatgttggc
    34681 gaaggtaaag atcttgattt tattcacgat aaggctttaa caataggtgc catagaatct
    34741 tatattttag atgttaaaga tgagttcgct gagcattttg ttttaccagc tttacaggca
    34801 catgcgatgt atgaacaaaa atacccgctt gtttcagcgc ttagtcgtcc aattattgcc
    34861 caaaaattag ttgaaatggc acatcaaaca ggtgcgacca caattgcaca tggctgtaca
    34921 ggtaaaggaa atgaccaagt gcgttttgaa gttgcaattg cagcactaga tcctgaatta
    34981 aaagtaattg cccctgttcg tgagtggaag tggcatcgtg aagaggaaat tacttttgca
    35041 aaagctaacg gcgtacctat tccagcagat ttagataacc cttattctat agaccaaaat
    35101 ttatggggac gtgccaatga atgtggtgtg cttgagaatc cttggaatca agcgccagaa
    35161 gaagcttttg gtattacaaa atcaccagaa gaagctcctg attgtgcaga atatattgat
    35221 attacgttcc aaaatgggaa gcctatagct ataaacaatc aagaaatgac actagcagat
    35281 ttgattttat cgctaaatga aattgcagga aaacatggta ttggacgcat tgatcatgtt
    35341 gaaaatcgtt tagtcggtat caaatcacgt gaaatttatg aatgtcctgc ggcaatggta
    35401 ctgttagctg ctcataaaga aattgaagat ttaacactag ttcgtgaagt gtctcatttt
    35461 aaacctatcc ttgaaaatga actatcaaat cttatttata atgctttatg gtttagtcca
    35521 gctacaaaag ctatcattgc ctatgtcaaa gaaacacaaa aagtggttaa tggtactaca
    35581 aaagttaagt tatataaagg ttctgcccaa gtcgttgctc gccactcatc taattcatta
    35641 tatgatgaga atttagcaac ctatacagct gctgatagct ttgatcaaga tgcagcagtt
    35701 ggttttatca aactctgggg acttccaacc caagttaatg cccaagtaaa caaaggataa
    35761 ataaaggaag ataagaggat gacaaaaaat cataagttat ggggtggtcg ttttgaaagt
    35821 agtcttgaaa aatgggttga agagttcggt gcatcaatca gtttcgacca aaaattagct
    35881 ccctatgata tgaaggcttc tatggcccat gtcacaatgt taggcaaaac ggatattatt
    35941 agtcaagaag aagcaggctt gataaaagat ggtttaaaaa tactgcaaga taaatacaga
    36001 gcaggtcaat tgacttttag catatcaaat gaagacattc atatgaacat agagagctta
    36061 ttaaccgctg aaattggaga agtagcaggt aagcttcata ctgcacgttc acgaaatgat
    36121 caagtggcta cagatatgca cctttatttg aaagataaat tgcaagaaat gatgaagaaa
    36181 ttgcttcatt tacgaacaac cttagttaat ttagcagaaa atcatatcta tactgttatg
    36241 ccagggtata cacatttaca acatgctcag ccgatttcat ttggacatca tctaatggct
    36301 tactataata tgtttacgcg tgatacagag cgattagagt ttaatatgaa acacaccaat
    36361 ctatcaccat taggagctgc tgctctagca gggacaacgt ttcctattga ccgccatatg
    36421 acaactcgtt tattagattt tgaaaaacca tatagtaatt cgcttgatgc tgtctcagat
    36481 cgtgacttta tcatagaatt tttatccaat gctagcattt taatgatgca tttatcacgt
    36541 ttttgtgaag aaatcattaa ttggtgcagt tatgaatatc aattcatcac attatcagat
    36601 actttctcta caggatcatc tattatgcct caaaagaaaa atcctgatat ggcagaatta
    36661 attcgaggga agacaggccg tgtttatgga aatttattta gtttactaac tgttatgaaa
    36721 tcattgccgt tagcttataa caaagattta caagaagata aagaagggat gtttgatagc
    36781 gtagaaacag tcagcatagc tattgaaatc atggctaata tgcttgagac aatgactgtg
    36841 aatgagcaca taatgatgac ctcaacggag actgattttt caaatgcaac agaattagca
    36901 gattatttag caagtaaggg ggtacctttc agaaaagcac atgaaattgt aggtaaattg
    36961 gttttagaat gcagtaaaaa tggttcttat ttacaggata tacctttaaa atattatcaa
    37021 gaaatttcag agttgataga aaacgatatc tatgagatat taactgcaaa gacagcagtt
    37081 aaacgaagaa attcattagg aggaacgggg tttgaccagg ttaaaaaaca gatcctattg
    37141 gcacgtaaag aattgaaagc ggaatgactg ctttctgaaa aaaccctcgt ttaactctgt
    37201 taagtttctc acgtattttc agtaaaaaac tagcattttt tatagaattg tgctagaatt
    37261 aacgtgtaag tgggcaacgc ccaaaaaata aataggaggc ctgaataatg gcaatcgttt
    37321 cagcagaaaa attcgtccaa gcagctcgtg ataacggcta tgcagtaggt ggatttaaca
    37381 caaacaacct tgagtggact caagctatct tgcgcgcagc agaagctaaa aaagctccag
    37441 tacttatcca aacttcaatg ggtgcggcta aatacatggg tggttacaaa ctttgtaaac
    37501 aacttattga aactttggtt gaatcaatgg gtattactgt accagttgct atccaccttg
    37561 accacggtca ttacgatgat gcccttgagt gtattgaagt aggttacact tcaattatgt
    37621 ttgatggttc acaccttcca gttgaagaaa accttgaaaa agcacgcgaa gttgttgcta
    37681 aagcacatgc taaaggtatt tcagtagaag ctgaagttgg tactatcggt ggtgaagaag
    37741 atggtattgt cggtaaaggt gaattagcac caatcgaaga cgctaaagct atggttgaaa
    37801 ctggtattga cttcttggct gcaggtatcg gtaacattca tggaccatac ccagcaaact
    37861 gggaaggact tgaccttgat cacttgaaga aattgactga agctgttcct ggtttcccaa
    37921 tcgtgttaca cggtggttca ggtattcctg atgatcaaat ccaagaagct atcaaacttg
    37981 gtgttgctaa agttaacgtt aacacagaat gtcaattagc tttctgtcaa gcaactcgtg
    38041 actacgctgt tgaattcaac gctaacgaag ctgaatacat gaagaaaaaa ctctttgacc
    38101 cacgtaaatt cttgaaacct ggtttcgatg caattacaga agcagttgaa gaacgtattg
    38161 acgtgtttgg ttcagcaaac aaagcataat taacataaac agataatagt taaaagctca
    38221 cttatggtga gctttttttg gattatatga ctttttatta aatagatact ctttaaagat
    38281 tgatatgctt aagataattt cgaataaatg ccgctgaatc tgtgagttgt tgctcttctt
    38341 ttgggcttag tgaaagagaa attctttcta caattcctac tctaccaata atagctggat
    38401 atccaaggta aaccccttcg gactcataga aggtagatac aggtagtaac tcatgggaat
    38461 catttaacac agttaaaacg atgcggacag ctgcggtagc gataccataa ctagtatatt
    38521 ttttaccaaa aaagacagtg tgcccgcctt ttaaagaagc ttcttttatc tgttctaatt
    38581 cttctgcttt taatagtgat atagctggtt gtccttttac agttacctga ctccaagcga
    38641 caaattggga gttaccatgc tctcctagat tataaccacc gatactcctt ggatcaactt
    38701 gtaggcgttg accaagcact cttttcatcc gagcagtatc caataaggtt cctgttccga
    38761 taacgcgtga tttaggaaat tgagtatatt cttgataaag ctgggtaatg gcatcaacag
    38821 gattagtaac gacaataagg atccctctaa aaccagatga ttttaacttt tggctaacgt
    38881 cgtagacttc tttactggta aatggtagtt cagcaaagcg attagcttct ttgttattct
    38941 gtagtgaaat atcacctaag gctgaaatca caacatcagc atctgacaac gcgtcatagt
    39001 cgtttaatac tagattagcg tgataagtag catttgccag agcatcttga aaatcaatca
    39061 tatcagcatt aaccttatca acattagtat caataaagac ataatcatca ccaatacctt
    39121 gtgcaataag agcgtgtgca acagttgatc caacgtgccc catgcctata attccaattt
    39181 tacgtgtcat atgaaacctc ctttgattat tgttgtctat tataatattt tgaaagaaaa
    39241 tgtaaactaa aaaatgaatt agaaggaagt gtgtcttatt ataaaaaata tgtaagcgat
    39301 ttagataaaa caaatgaaaa agtaattttg aaaagaactt gctagaagaa gtaagttgtg
    39361 atagaatagt ctagtatgtg gtgctagcac atccgtaaat attgatcaat ataggaggaa
    39421 ctcgtaaaat ggctaaagta tgttatttca caggacgtaa aacagtttct ggtaacaacc
    39481 gttcacacgc gatgaaccaa acaaaacgta ctgttaaacc aaaccttcaa aaagttactg
    39541 ttcttatcga cggtaaacca aaaaaagttt gggtttcagc tcgtgcgctt aaatctggta
    39601 aagtagaacg cgtttaataa actgagaaag ccttagggct ttttttagtg tttctaaggc
    39661 tgatttgata gttagatgcc tctatttttt tacaataata gtgagatatg atagaataga
    39721 gtgataaaaa ctttttgagg tagtagatat gactgtaaaa atcaatacaa gagatggtca
    39781 aattgaactc tctgatgacg ttattgcaac tgtcgttgga ggttcagcaa cggaaatttt
    39841 tggcgttgtt ggtatggcaa gtaaaagtgc tatcaaagat aactttcaag cacttttgcg
    39901 taaggaaaac tacgcaaaag gtgttgttgt gaggtctagt gaagcgggca tcgtagtaga
    39961 tgtttatact gtcatgagtt acggcgtcaa aattagtgaa gtttcaaaaa atattcaaga
    40021 gcgagtgaaa ttcaatctcg aaaatcagct tggtttatct gctgatatgg tgaatgttta
    40081 tgtgcaaagt attaaggttg taggagaaga ttagtgtcaa agattacgac aagtttattc
    40141 caagaaatgg tacaagctgc cagtacacgt ctgagtaaac aggcagagta tgtcaactca
    40201 ttaaatgtat tcccagttcc agatggtgat actggtacga atatggggat gactatggaa
    40261 aacggagcca aggaagtttc ggacaaacca gcgacgactg ttggagaagt tggtcaaatt
    40321 ctttctaaag gtgtattgat gggagcacgt ggaaattcag gtgtaattac atcacaatta
    40381 ttccgtggtt ttggtcaaag tatcaaggat aaagaagaat taacgggtca agatttggca
    40441 cacgctttcc agaatggtgt tgaagtagct tataaagccg ttatgaaacc agttgaagga
    40501 actatcttga cagtatcacg cggtgcagct accgctgctc taaaaaaagc tgaagaaact
    40561 gatgatgcag ttgaagttat gcgcgcaact ctcaaaggtg ctaagcgtgc tcttgctaaa
    40621 acaccggata tgctacctgt tcttaaagag gttggtgttg ttgattctgg tggtcaagga
    40681 ctcgtcttta tttacgaagg tttcctctca gcactgactg gggaatatat cgcatcagaa
    40741 gattttaaag caactcccgc aacaatgact gaaatggtta atgcagagca ccataaagct
    40801 gtagtaggcc acgtagccac agaagatatt aaatatggct attgtacaga agtcatggtt
    40861 ggtttaaagc aaggaccaac ttacgttaag gaatttaact acgaagaatt tcaaggttat
    40921 cttagcaatc taggggattc tttactagtt gttaatgatg atgaaattgt caaggttcat
    40981 gttcatacag aagatccagg tcttgttatg caagaaggtt tgaaatatgg atcgcttgtg
    41041 aaggttaaag ttgaaaacat gcgtaaccaa catgatgccc aaatgcaaaa ggttgaagtt
    41101 gaagaaactg tcaaagaaac taaagaatat ggtattattg ctgttgtagc tggtgacggt
    41161 ctagctgaga tttttaaatc acaaggtgtt gattatatca tctctggcgg ccagactatg
    41221 aacccatcta cagaagatat tgtaaaagcg attgaaaaag taaacgctag aaatgttatt
    41281 attttaccaa acaataagaa catcttcatg gctgcacagt cagctgcaga tgtcgttgat
    41341 attccagcag cagtagtaga gacacgtaca gttccacaag gatttactag cttacttgct
    41401 tttgatcctg ctaagtctct tgaaactaat gtagctgata tgacaaatag cctctcagat
    41461 gtcattagtg gtagtgtgac tttagctgtt cgtgatacaa cgatagatgg ccttgaaatt
    41521 catgaaaacg acatccttgg gatggttgat ggtaaaattc tagtatctac accagatatg
    41581 gaaaaagcac ttaaagacac ttttgataaa atgattgatg aagatagtga aatcgtgaca
    41641 atctatgttg gtgaagatgg taagcaagca ttagctgaga ccttgtcgga atatcttgag
    41701 gaaacttatg aagatgttga agttgagatc caccaaggag atcaacctgt atatccatac
    41761 ctcatgagcg ttgaataatt gataaaataa aaaattagga gagatacttc taatttttta
    41821 ttttatcaga gtttctattt tagtatgata aagcatcagt attttgaaaa ttttgacaaa
    41881 ttgtgttaga attgttttaa aacattcata caaaggagat ttatgatgtc attaattatt
    41941 cttacggtta ttttggtttt agtgattgtt ctgttgataa cttctttata cgttgttaaa
    42001 caacagacag tagccatcat tgaacgcttt ggtaaatatc aaaagactgc tactagcggt
    42061 attcatattc gtgtaccatt gggtatcgat aagattgcag cacgagtgca gttacgtctt
    42121 ttacagagtg aaattatcgt tgagacaaaa acaaaagata atgtttttgt cactttaaat
    42181 attgctacac aatatcgtgt caatgaaaat aacgttacag acgcttatta caaattaata
    42241 aagccagaag ctcagattaa atcttatatc gaagatgctt tgcgttcatc agtacctaaa
    42301 cttacattag acgagctatt tgaaaagaaa gatgaaatag ccttggaagt tcaacatcaa
    42361 gtggcagaag aaatgtcaac atacggctac atcattgtta agactttaat tactaaggtt
    42421 gagccagatg cagaagtaaa acaatccatg aacgaaatca atgctgcaca acgtaaacgt
    42481 gtagcagctc aagaattagc gaatgctgat aagatcaaaa ttgtaactgc tgcagaagca
    42541 gaagcagaaa aagatcgtct acatggtgtg ggtattgcgc aacaacgtaa agcaattgta
    42601 gatgggttag ctgattcgat tcaagaattg aaagatgcta acgtgacatt gacagaagaa
    42661 caaatcatgt ctatcttgtt aacaaatcaa tatttagata ctttaaatac atttgctatt
    42721 aatggtaatc aaacgatttt cttaccaaat aacccagaag gtgttgaaga tattcgtaca
    42781 caagttttat ctgctcttaa aacaagataa tgaacttttt atagaagcaa aataaccaga
    42841 gcatttgctc tggttatttt gtgtctgact tatttattat aattggagag acggaaaaaa
    42901 gttatttttt accgtaacgt ttgtactcat gaatgatttt cagagcacgt tttctagttt
    42961 taatgttagg acttttaagt ctgcgatagg ctgacgatag gtctgttttt tcagccatga
    43021 ttacctccta tagacaattt attaacgtta acagacacca gtataccaca aaaaaattaa
    43081 ctggtaaagt actttgttga taaatttaaa aataaatagc aaagtaatta aatatcaatc
    43141 aaaagtaata agttcaaaga tatattaaac ctttattaat gaggtttgga tttgacaagt
    43201 gatgttaagt gaaagatgaa tgaggaaaat acccaacgag aaagtagcta atggctatca
    43261 ttgtgcatat aaaaaaacga tcgatgagtg atcgtttttc agcaaactaa ataattattt
    43321 ttaatgtaat tgaatgaaaa tttatacttg tgaaagtgta tcggttaagt taataaggtc
    43381 agtttttatt tatcgtaagc acttaggaag tgttttggag catacccacg agagtagaca
    43441 aatccggcat cacgtgaagg ataagacact gtgtctgcct tcgcaagact tgctaaagta
    43501 gctaataata gagtgacagc tgaagctgct gttaaaacac tgattttaag tttcattata
    43561 aaatcctttc aaagataata aactattctt gcacagtggt tagttcattt tgtgcatact
    43621 atattatatc atcaaaagct cattcgaaca acatgttttt aattttaaac aaatatatga
    43681 ttaatcaaat atcgcttaaa attaaaaaat gagatttagt aacttattta atagtataca
    43741 atccaataac gactgtctaa tttgatctat agttggagct agtcgtggat ttcactaaag
    43801 atgtagcaat aaaaaatccc ctccagttcc attgcaatga tgacaatgac aaaacctgta
    43861 ggggtataaa tagtctatat ttaaatgtga ataaatttta ttttaagaag cgttgtagaa
    43921 attccttagt tctttcttgt gttggatttt cgaaaagttg tttaggagta ccctgctctg
    43981 caatgattcc tttgtccatg aaaataacgc ggtctgatac ttctttagca aattccatct
    44041 cgtgtgtcac aattatcatt gttaatccag atttagctag atcttgcatt gttttaagga
    44101 cctcaccaac catttctgga tccaaggctg aagttggttc atcaaaaagt atagcttctg
    44161 ggttaacaga aagtgctcgg gcgattgcga cacgttgctt ttgcccacct gataattgtt
    44221 tgggtttagc tttccaatat tgctcagtca tgccaacagc atttaagttt tctttagcaa
    44281 tcttttcagc ttcttggcgt tcacgtttta gaacagtagt ctgtgcgaca atagcattct
    44341 ctaagatatt taaattttca aagaggttaa aagattgaaa gaccatccca agtttttcac
    44401 gataattatt taaatcatag cctttttcaa gaacattgtg accgtgatat aagatttctc
    44461 ctccacttgg ttcttctaag aggttaatag aacgtaggaa agttgatttt cctgatccag
    44521 aagaaccaat gatagaaata acttcgcctt tgttgaccga aagcgaaata tcttttaaca
    44581 cttcattgct tccgtaagat tttttcaaat gtttaatttc taaaatagct tgtgtcatac
    44641 caacctccta gacttgtaat tgattcgcac cggttgtgta attatctgaa tcaaaacgtt
    44701 tttcaatgta acgtaagata cgtgttacgg tgaaggttaa gatgaagtaa ataatcgcaa
    44761 taatggtgaa tgtttggaaa tattggtagg tttgtgttgc aacagtatta ccagagaagt
    44821 agagttcgac aacagagata acgttcagta cagaggtatc tttgatattg ataacaaact
    44881 cattaccagt agctgggagt atgttacgta caacttgtgg tagaacaatt tttcgcatgg
    44941 tttgtccgtg ggtgaaacca agtgcagttg cggcttcaaa ttgtccttta tcaacagaga
    45001 aaataccacc acgtacaatt tcgctcatat aagcaccagt attgattgaa acaataaata
    45061 tagctgctag tgtacggtcc agcgaaactc caaaagcttg tgctgttccg tagtagataa
    45121 ccatcgattg tacaatcata ggtgtcccgc ggaagacctc aatatagatg ttaagtaacc
    45181 aacctaataa tttttgtaag gcagccttta acttattgtc agattttggt gctgtacgga
    45241 aaacaccgat gagaaggccg ataaataatc cgacgattgt tcctaccata gaaattagga
    45301 gggttgtagc tgttcctctg aggaattgag aaccattgtt ttttaaaata gttgccatct
    45361 gttcaaagaa gtttggtttc ccattttttt cttttttaac ggatggctgt tctttaatca
    45421 tcttatccat caaggcgatt tgtttatctc tagaaatgct ttcgagaact tgattaactt
    45481 gattaatacg attgtcacct ttacgcatcc caacagaaat atttgtatcg gcatcagatg
    45541 ttttaaaccc ttggtgcagt tcaatcattt ttaatttagg attagcagtt tgagcggaag
    45601 ttgcatctgg acgttctgaa acataggcgt ctacaacacc agattctaca gcttgtctta
    45661 attgattgaa gtcccccatg gcaacttctt ttttgacacc attgatttga tcaatgaggt
    45721 tgtataagta gactccttgt tgggcagtta ctttagcatt tttgaaatca ctaatatttt
    45781 tagcattagt atattttcct tcagcattaa caactagagt aggttctgaa atgtagtaag
    45841 gctttgaaaa attaatttct tttttacgtt cttcggtagg actcattcct gcaataatca
    45901 tatcgagttt tccagaagtt agtgctggaa caaggccttc ccattttgtt ttgacaacaa
    45961 caactttttt atttaacttt ttagctaact ttttagcgac ttgtacatca taaccattag
    46021 catattgatc agttccttca ataggaactg caccatttgt gttatcattt tgtgtccagt
    46081 tgaaaggagc ataggctgcc tccataccga cacgtagata ttcatctgct tgtatacttg
    46141 tcatacctcc aaaggtgata agcaaagcag tgaataaact tagtaatatt tttttcattc
    46201 aacagcctcc gataagttac ttcctcctat tttaccgaaa aacaaggtcg atttcaatat
    46261 cattatctta ttgaaagcga ttaattgtat ttttatgtta aaatggtata caaacaaagg
    46321 aaggaggatg ataatgaaga aatgtttttt ggctatttgt ttagctctta gtttttttat
    46381 ggtttcagtt caagcagatg aggtggacta taacattcct cattatgagg gtaatctaac
    46441 tattcacaat gataatagtg ctgattttac agagaaggtt acttaccaat ttgattcgtc
    46501 ctataatgga cagtatgtca cattaggtac ggcgggtaag ttacctgaca attttgatat
    46561 taataataag ccacaggttg aagtttcaat taatggtaaa gtaaggaaag ttagttacca
    46621 gatagaagat ttggaggatg gctaccgttt gaaagtgttt aatggtggtg aagcaggtga
    46681 tactgttaaa gtcaatgttc agtggaaact aaaaaatgtt ctatttatgc ataaggatgt
    46741 tggtgaactt aactggattc ctattagcga ctgggataaa acgttagaga aagtagattt
    46801 ttggatatca actgacaaaa aggttgctct ttctcgtctt tgggggcact tgggttatct
    46861 taaaactcct cctaaaataa gacaaaataa taatcgttac catttgacag cttttaatgt
    46921 aaacaaacga ttagaatttc atggttattg ggatagatct tattttaatc tacctacaaa
    46981 cagtaaaaat aattacaaga aaaaaattga atatcaagag aagatgatag agcgtcatgg
    47041 ttttatccta agtttcttgt taaggatatt attaccttca ttctttatta ttgtgacact
    47101 attcatctca attagggtgt tcctgtttag aaaaaaagtt aataaatacg ggcaattccc
    47161 taaggaacat catttatatg aagcacctga ggacctttca ccactagagt taactcaaag
    47221 catttatagt atgagtttta aaaattttca agatgaggag aagaaaactc accttatcag
    47281 tcaagaacaa ctcattcagt caattctatt agacttgatt gatagaaaag tattgaatta
    47341 tgatgataac ttgttatctc tagctaactt agatagagct tctgatgcag aaatagattt
    47401 tatagagttt gcttttgcgg attctacgag tttgaagcca gatcaactct tttctaatta
    47461 ccaatttagt tataaagaaa cactacgtga actgaaaaag cagcacaagg cttcagatct
    47521 gcaaactcaa atgagacgcc gaggaagtaa tgccttatca agaattacgc gtctcacaag
    47581 gttgatttct aaagacaata taaactctct tagaagaaag ggaatttcat ccccttatcg
    47641 taaaatgtct tcagaagagt ctaaagaatt atctaggttg aaaagattca gttacctatc
    47701 acctcttatt tcttttgttg ttataattta tacgcttttt ttaaattatt ttacctattt
    47761 ctgtatctat ctcttattgt ttggtgttat cctgttgttg aataaaatca tttttatgat
    47821 gacaagaaaa ataagtaacg gttatattgt aactgaagat ggagcaagtc gtgtctacca
    47881 atggactagt tttaggaaca tgctaaggga tatcaaatcg tttgatcgtt cagagttaga
    47941 aagtatcgta ttatggaatc gaatattggt ttacgctact ttattcggct acgctgaccg
    48001 tgttgagaaa gcactcagag tgaaccaaat agatattcca gaaagatttg caaacattga
    48061 tagtcatcaa tttgcgattt cagtcaatca atctagtaat catttttcaa cgataactga
    48121 agatgttagt cacgcttcta attttagtgt taattcaggc ggttcttcag gtggtttctc
    48181 aggcggcgga ggcggcggag gtggtggtgc cttctaattt tccaaaagta tggtataata
    48241 aagaaaaatt tatggagtta ttatgttaat aattgaatta ctcaaagccc tatttttagg
    48301 agtcgttgag ggagtgacag aatggttacc tgtttctagt actggccatt taatcttggt
    48361 tcaagagttt atgaagttga accaaagcaa atcctttgtt gaaatgttta atattgttat
    48421 acaacttggg gcaattatgg cagttattgt catttatttt aaacgcctta acccatttca
    48481 gccaggtaaa tctgctagag aaattcgttt aacttggcaa ttatggttaa aagtagtgat
    48541 agcttgtatt ccatctattt taattgcttt accatttgat aattggtttg aagctcattt
    48601 taatttcatg atcccgattg caatagccct aatcttttat ggttttgtct tcatatgggt
    48661 tgaaaaacgt aatgcacacc tcaaaccaca ggtaaccgaa ttggcaagta tgtcttacaa
    48721 gacagctttc ttgattggat gcttccaggt tctcagtatt gttccgggaa ccagtcgttc
    48781 tggagctact attttaggag caattattat tggaactagt cgttcggtcg ctgctgactt
    48841 tactttcttc cttgccatcc caactatgtt tggttatagt ggacttaagg cggttaaata
    48901 ctttttagat ggtaacgtct tgagtttaga ccaatcttta atacttttag tagcaagtct
    48961 gacagctttc gtagttagtt tatatgttat tcgtttcttg acagactatg tcaaacgaca
    49021 cgatttcacc atctttggta agtatcgtat agtcttagga agtttactca tcctctactg
    49081 gttagttgtt catttattct aaaatataac ataaaaagct gagtattcct cagcttttta
    49141 gcgtgttagg ctgtgaaaaa ttgctaaaaa atagcttttt cggtataata agaaaactga
    49201 atactcgtat gaggtgatga tatggaaatg aaacaaatca gtgaaacaac actgaaaatt
    49261 acaattagta tggaagattt agaagatcgt ggtatggagc tgaaagattt cctaatccct
    49321 caggagaaga ctgaggaatt tttctattct gtcatggatg aattagactt gccagaaaac
    49381 tttaaaaata gtggtatgtt aagttttcga gtaacaccta aaaaagatcg cattgatgtt
    49441 tttgttacaa agtctgaatt aagtaaagat ttaaatttag aagaattagc agatttgggt
    49501 gacatttcaa aaatgtctcc agaagacttt tttaaaacat tggaacaatc gatgttggaa
    49561 aaaggggata cggatgccca tgccaaatta gcagaaattg aaaatatgat ggataaagca
    49621 actcaagaag tagttgagga aaatgtttct gaagaacaac ctgaaaagga agtagaaacg
    49681 attggatatg ttcactatgt ctttgatttt gataatattg aagctgtagt tcgattttca
    49741 caaacgattg attttccaat agaagcttct gaactctata aaaatggtaa aggctatcat
    49801 atgacaattt tgctagactt ggaaaaccaa ccaagctact ttgctaattt gatgtatgct
    49861 cgaatgcttg aacatgctaa tgtgggcaca aaaacacgag cttacttgaa agagcatagt
    49921 attcaattga ttcatgatga tgctatttca aaattacaaa tgattgagat gggataaaat
    49981 gatacctttt acaattgagt atatttttgt attgattgga gcatttttat tatcaatcat
    50041 tctaactcct attattcgag ttatatcttt gaaggtagga gcggttgata aaccgaacgc
    50101 tcgaagaatc aacaaagtac caatgccaag tagtggtggt ttagcaatat ttctctcctt
    50161 tgttgtaaca acattatttt tcatgccaat ggcggccagt agacacttta tagaagtctc
    50221 ttattttcac tacattttgc ctgtaattat tggcggtctc gtagttacaa caacaggatt
    50281 catagatgac atctttgaat taagacctcg ttataaaatg ttgggaatta ttattgcagc
    50341 tattatcata tggaaattta cacattttag atttgatagt tttaagattc caataggtgg
    50401 tccactcttg gagtttggtc ctattttaac tttctttttg acagttttat ggattatttc
    50461 aatcactaat gctatcaatc tgattgatgg tctagatggt ctagttagtg gtgtttcaat
    50521 tatcagtcta gcaacgatgg cagtggtttc ttatttcttt ttacctaaaa tagatttttt
    50581 tctcacgcta actattgtta ttttaatcgc ctcaatagtt ggttttttcc cttataatta
    50641 tcatccagcc attatctacc taggtgatgc tggtgccttg tttataggat ttatgattgg
    50701 tgtattatcg cttcaaggtt taaaaaactc cacagctgtt gcagtgataa cacctgtcat
    50761 tattttagga gttcctattt tggatacagc tgttgctatt gtgaggcgta aattatcagg
    50821 gaaaaagatt tctgaagctg ataagatgca cctgcatcac cgattgctat ctatgggatt
    50881 tacacataga ggagcagttt tagttgttta tggtattgca attatctttt cgcttatcgc
    50941 tttgctttta aatgtttcta gtcgtattgg gggaattttt cttctattgg cgctactatt
    51001 agcgatggaa atttttatag aaggtttaaa tatttggggt gagaatcgga caccactatt
    51061 taatctctta aagttcattg gcaacagtga ttacagacaa agtgttattg ctaaatatag
    51121 cgataagcat caaaaataaa agcctgaaaa ggctttttta gttttaatca tttagatagc
    51181 agtcacacct catttttgct ataattattt agaatataat atcgggtgag tgccctataa
    51241 tatcttcttt aaaccataga gaaatatcac atattctctg ttaaccaaga aaaggagtgt
    51301 acaatgtctg tacttgaaat caaaaatctt catgtttcta ttgaagataa agaaatttta
    51361 aaaggtctta atttaacgtt gaaaacagga gaaattgctg ctatcatggg tccaaatggt
    51421 actgggaagt cgacactttc agcagctatt atgggaaatc ctaattatga agttacagca
    51481 ggtgaaattc tttttgatgg tgaagatatt ttagagctag aagtggatga acgtgctcgt
    51541 ttgggtctat ttcttgccat gcaataccct tcagaggttc cagggattac taatgctgaa
    51601 tttatccgag cagctatgaa tgcgggaaag gctgatgatg ataaaatttc catccgccaa
    51661 tttatcacta agttagatga aaagatggaa ctacttggca tgaaagaaga aatggcggaa
    51721 cgttatctta acgaaggttt ttcaggtggt gagaagaagc gcaatgaaat tttacaactc
    51781 ctaatgttag agcctaagtt tgccctcttg gatgagattg actctggcct tgatattgat
    51841 gctcttaaag tggtatcaaa gggagtcaat gaaatgcgtg gtgaaggttt tggtgctatg
    51901 attattacac actatcaacg ccttttgaat tacattacac cagataaagt acacgttatg
    51961 atggacggga aggtagtatt gtcaggagga ccagaattag cggttcgcct tgagaaagaa
    52021 ggttatgctc aaatcgctga agagcttggt ttagagtata aagaagaagt ctaatttgtc
    52081 acttcaacta gaagagaaaa ggagttacaa atgtctaaag aagctatatt aaatttttta
    52141 caagccaaag gtgagcctac ttggttacaa gaattacgac taaaggcttt tgaaaaaata
    52201 gaagaactag aattaccagt aattgaacgt gtaaaatttc atagatggaa tttaggtgat
    52261 ggaaccattt tagagaatga ctatacagct aacgttccag atttcacaga gttaggaaac
    52321 aatcccaagt tggttcaaat tgggacccaa accgtccttg aacaagttcc tatggaatta
    52381 atagagaaag gcgttgtctt tacagatttc tattcggcct tggaagaaat tccagaagtc
    52441 atagagcgct actttgggaa agcacgtcct tttgaagaag atagacttgc tgcctatcac
    52501 acagcttact tcaacagtgg tgcagtgctt tatattccag ataatgttga aattactcaa
    52561 ccaatcgaag gtctttttta tcaagatagt cagtctaaag taccatttaa taagcatatc
    52621 cttcttattg ttggaaagaa tgcaaaagtt agttacttag agcgatttga gtcaattggt
    52681 gatgggacag aacgaacaag tgctaatatt tctgtagaag taattgccca agcaggtagc
    52741 caaattaaat ttgcttcaat tgatcgcctc ggagaaaatg tgacaacgtt tataagtcgt
    52801 cgtggtcgtc acagtagcga tgcaacaata gattgggccc ttggggtgat gaatgaaggc
    52861 aatgttgttg ctgactttga ttctgattta attggtgatg gaagtcacgc taatttaaaa
    52921 gtagttgctg cctcgtcagg gcgtcaagtt caaggaattg atacgcgtgt tacaaactac
    52981 ggctgtaatt cagtaggaca tattttacag catggggtta ttttagagcg tgggactctt
    53041 acttttaatg gtatcggaca tattataaaa ggtgcaaagg gagcagacgc ccaacaagaa
    53101 agccgagttc tcatgttatc agacaaagca cgttcagatg ctaatcctat ccttttgatt
    53161 gatgaaaatg atgttacagc tggtcacgca gcctcaattg gtcaggttga cccagaggat
    53221 ttatattatt tgatgagccg aggcttgaac caaaaaacag cagagcaatt agttattcgt
    53281 ggatttttag gaacagtcat tgctgaaata ccagtaaaag aagttcgcga tgaaatgatt
    53341 gccgttattg atacaaaact agaaaaacgt taatatgaca ttactagata gttataaact
    53401 aaagcaagat ttcccaattt taaatcaatt agttaatgat gagccgctta tctatttaga
    53461 taatgcagca acgacacaaa aaccgaatca agttttagaa gctttgcgag actactacca
    53521 aaacgacaat gcaaatgtcc atcgtggtgt tcatacttta gcagaacgag cgactgctca
    53581 gtacgagaat gctcgtgaaa aagcaaggca gtttttaaat gctaaattaa gtaaagaaat
    53641 tttattcaca cgtggcacca caacaggatt aaattgggta gctaagtttg ccgaaagtat
    53701 tttagaacga ggtgatgagg tacttatttc cattatggag catcattcta atattatccc
    53761 atggcaacaa gcttgcgaga gaactggcgc taaattggtt tatgcctatc taaaagatgg
    53821 ttctttagat ttagaagatt tctataataa attatcctct aaaactaaat ttgttagttt
    53881 ggcacatatc tcaaatgtac ttggctgtgt tacaccagta aaagcaatag cagaacgtgt
    53941 acatcaagtt ggggcataca tggttgttga tggagctcaa tcagcgccac atatggcgat
    54001 tgatgttcaa gaccttgatt gtgatttctt cgccttatct ggccataaga tgcttggtcc
    54061 tactggtatt ggtgttcttt atgggaaaga aagcatttta gataagatgc cacctgtcga
    54121 atttggtggt gaaatgattg attttgtcta tgagcaaagt gcaacatgga aggaattgcc
    54181 ctggaagttt gaagctggaa cgcctaatat tgctggagcg atcgcttttg gagaagcttt
    54241 agattattta acggatgttg gtatggatga gattcatcaa tatgaacaaa gtttagtttc
    54301 ttatgtgttg cctaaattac aggctatcga tggtttaact atttacggac ctagtgatgc
    54361 tgaatctcat gtaggcgtta ttgccttcaa tttagaaggt ttacacccac atgatgtggc
    54421 aactgcaatg gattacgaag gagtggctgt tcgtgcaggt catcattgtg cacaacccct
    54481 tattaatcat cttggtatcc attcggcggt tcgagcaagt ttctatttct ataataccaa
    54541 agaggattgc gacaaattgg tagacgctat ccaaaaaact aaggagtttt tcaatggcac
    54601 tctctaaatt agataatctc tacatggcag tggtagctga tcattcaaaa caccctcatc
    54661 atcatggatt tttagaaggc gttgaacaag tgcagttaaa taatcctaca tgtggagatg
    54721 ttatttcctt atctgttaaa tttgatggaa atattatttc agatattgca tttgctggta
    54781 atggttgtac aatttctacg gcttcatcaa gcatgatgac agatgcagtt attgggaaaa
    54841 caaaagaaga agcattgcag cttgctgatg tattctccaa aatggttcaa ggcgatcaaa
    54901 atccaaaaca agagaaactt ggggatgcag agtttctagc tggggtatca aaatttccac
    54961 aacgtatcaa atgtgctacg ctttcgtgga atgctttaag aaaggctatt gagagagata
    55021 atcaggcaga actctgacaa ccatagttga aggaattaca tttaaacaac ttttactgac
    55081 ataaggtagt ctagtagaag aatgtcttaa atttaaatat taaagaaagg ataagtatgt
    55141 ccgaaataaa tgaaaaagta gaaccacagc caatagatct tggggaatat aaatttggat
    55201 ttcatgacga tgtaaagcca atttattcta cgggaaaagg tctaaatgag gctgttattc
    55261 gtgagttatc tgcagctaag ggtgaacctg agtggatgtt ggactttcgt ctaaaatcct
    55321 tggaaacgtt taataaaatg ccaatgcaga cctggggagc agatttatca gatattgatt
    55381 ttgatgatat tatttattat caaaaagcat ctgataaacc tgcgcgtgat tgggatgatg
    55441 ttccagaaaa aatcaaagaa acttttgaaa gaattgggat tccagaagct gaaagagctt
    55501 atcttgcagg agcatcagca caatatgaat cagaagtggt ttatcataat atgaaagaag
    55561 aatatgataa gctgggtatt gtttttacgg atactgactc tgcacttaaa gagtacccag
    55621 agctattcaa aaaatatttt gctaaacttg tccctccaac agataataaa ttagctgctc
    55681 tgaactctgc tgtatggtca ggtggaacat ttatttatgt ccctaaaggt gttaaggtgg
    55741 atattccact tcaaacttac ttccgtatta ataatgaaaa tactggacaa tttgaacgta
    55801 ctctcattat tgtcgatgag ggagcaagtg ttcactatgt tgaaggttgt accgccccaa
    55861 cttattcttc aaatagttta catgcagcta tagttgaaat ttttgcactt gatggagctt
    55921 atatgcgcta tacgactatt caaaattggt ccgataatgt ctataattta gtgacaaaac
    55981 gtgctaccgc taaaaaagat gcaacagttg agtggataga tggaaatcta ggtgctaaaa
    56041 caacaatgaa atacccatcg gtttaccttg atggtgaagg agcacgtggc acgatgttgt
    56101 ctattgcttt tgcaaacaaa ggacaacacc aagatacggg tgcaaagatg attcataatg
    56161 ctccccatac tagttcatcc attgtctcta aatcaattgc taagggtgga ggaaaagttg
    56221 attatcgagg tcaagtgaca tttaataaag attccaaaaa atcagtgtca catatagaat
    56281 gtgacaccat attgatggat gatatttcaa aatcagatac cataccgttt aatgagattc
    56341 ataattcaca ggttgcttta gagcatgaag caaaggtgtc taagatttct gaagagcaac
    56401 tgtactactt gatgagtcga ggtttatctg aagctgaggc aacagaaatg attgttatgg
    56461 ggtttgttga gccctttacg aaagaattac caatggaata tgcggtagag ttaaaccgct
    56521 taatttccta tgaaatggaa ggttcagttg gttaattgaa gatttcaaga gataataaaa
    56581 agcccctatg ttattgatga agggggcttt tatattagca cagttattat ctagagcgct
    56641 ttataagtaa tactatccca gctaaaagac aaaggatagc gatggtgccc agaacaatta
    56701 aaatagatac aaaatgtgat tgaataaatt gatctgtttt tgtccaccac tgttcttttg
    56761 tttgaggctg ttcttgaata gatttggttt tggttttagt aacttctttt ttaggtttaa
    56821 cggcttttaa tacaggagtt ttagaagaaa tatttttagc gtctttaaaa cctttttcta
    56881 caagagcgtt gacaaacgga tgacgatagt actctccgtc ttggtctgac caatcaccaa
    56941 ctcctaaaac cacagttatc aagcgagtat tctggcgttt agctgtaact aaggcattaa
    57001 aagcagcgct agggctagaa ccagttttta agccatctac tccttctaat ccaaatttag
    57061 cgccgggggt agagtagtta taagttgtaa atgtttcttc ataaggagtt ccgaccatgg
    57121 ccttgacttc aggatatttt gtatagttca gtatatcagg gtatttttta aggaaatgat
    57181 aggttaaaat tgatagatca cgtgcagtcg taacgttagt agcattgtta tcgtattctt
    57241 ttggggagta aagtccatta aaagcactcg ctaccgcccc actggggtta taaaagtgag
    57301 tttttgtcat accgagtttc ttggcggttt cattgattcg tttaatgaag gcgtcaggat
    57361 tgttttgtga taagtggtta gcaatcataa tagttgctac attagatgac gggacagctg
    57421 tcatagtaat cagttcacga ataggataag caaccccagc atgaatatta ttattactaa
    57481 tttcataaat cttacttatg gcttggtctg tttctgttgc agtcactgtg gtgttgaggc
    57541 ttgtttttcc tttagctaag tcttcaaata gtaaatataa agtaaacatt ttagacatgc
    57601 tagcgggatc acgttctaaa tcagcgttat cttcccacaa aatatgacct ttattgtcaa
    57661 taacgataga cgctttaggt ttattaatat ctttaacatc atatccggct tctcgggtaa
    57721 tttgcataat atctttatgt tcttcagctt gcacagagtt tacacaagtc agggataata
    57781 aaatgcagag gaaagatacg attaatttag gcataggtaa ctcctgaaaa ctttttatta
    57841 atattataac aaaaaatcac taataaaaaa gacatttcat tagtgatttt taattttata
    57901 tattctccta aacctagcta aagaaactgc tgtgcagcaa ctccttgtgt caaagggaga
    57961 ttactattaa agtttttcgt taacgtagcg cacaaaatga ttccaccaca ctttgaggaa
    58021 gaaactgcgc tcaatactat cttttgccac taaatgaacg ctaggttcct ttatgagata
    58081 gccttttcca accttattaa ggtcaacgta ataagctttc cctaggttct ctttttttgt
    58141 aataggagca gtaagctctt ttttaaagtt aatttttaac tgattttgtt tagtgatatt
    58201 tttcttctgt actactttca aatcgttttt agcaacagcg atgaccgatt gttcttttcc
    58261 gtctctcaca cttgcttctt tacctttata tgcagctcct ttagctaata caaggttagg
    58321 ttcgtaggtg tttgtgatat agttcaagag agagttagtt gctgtaaagc gagcatattt
    58381 gtctttatcg gccttatcag catgcataat aacagtgagt agtctcattc cactttcagt
    58441 agatgtagca ataaaagatt ggccagctaa ttccgttgtc ccagttttca aacctgtaat
    58501 acctggtcta aagacaggca tatctggtag catgtagtta taagaatgca taatatcttt
    58561 atcaaattta gcaacggact tactagtaat cttaagaatg gaaggatatt cgttgaccaa
    58621 atggtaggca acaatagcaa tatcacgtgc actcatttta ttttcgtcgt tttgtgacga
    58681 ttttggataa atgtgattgc ctaacatact attatttaag ccagaagcat tgactaggtg
    58741 gctatcatga attccccact tttccaattg agcagtcatt ttatcaacaa atttactttc
    58801 ggttcctgaa atatgttcag ccaaagcaat ggctgcactg ttagcactag aaatcatggc
    58861 agcgtccacg agctgtttaa cagtatagcg ccttttttct aaaggaacat tactagcatc
    58921 agattcgcgt gttagttgat aagggtagtc agatatattt actttggtat tccacttgag
    58981 gttaccgtta tcaatttctt tatagaccat ataaacggtc attattttag tcaaggaagc
    59041 aatagcagcg ggtttgttag catctttttc atacaagatt tttcctgaat ctaaatcaac
    59101 tgccaaggca tgtttagcgg aagcattaaa agattcagca gagatggttg gcataaaaaa
    59161 aatgcaacta agtaatagaa tagaagtaat aatttttttc aaaataacac ctttagaaat
    59221 tttttaaact ttgtccctcc tattatatca tatcactata tcatactagg aattattatt
    59281 tttgataatt aaataaagaa aacgtttgtt ataaaaatgt aaaaaaatgt aaaaaaatga
    59341 aagttaaaaa attatctgaa aattttgcgt ttttgtcttt tcttaagaat tttttgtggt
    59401 ataatgttag tcactagata aatgaattag gagaacaaag ctatggcaaa acagaaaaat
    59461 aactggcgcc gtgttggagt tggtgtcctt acacttgctt cagttgcgac tcttgctgca
    59521 tgtggaagta aatcagcttc ccaggattct aatggagcga ttaattgggc tattccaaca
    59581 gaaatcaata cactagattt atctaaagtc acagacactt actcaaatct agctattggt
    59641 aactctagta gtaatttcct tcgcttagat aaagatggaa agacaagacc agatttggct
    59701 actaaagtag atgtttcaaa agatggctta acttatacag ctacattacg taaaggcttg
    59761 aagtggtcag atggcagtaa acttactgca aaggattttg tttattcatg gcaacgttta
    59821 gttgatccta aaacagcttc acaatatgct taccttgctg ttgaagggca tgtgcttaat
    59881 gccgataaaa tcaacgaagg acaagagaaa gacttgaata agctaggtgt taaggcggaa
    59941 ggcgatgaca aagttgttat tactttatct agtccgtctc cacaatttat ctactacctt
    60001 gcattcacta acttcatgcc acaaaaacaa gaagttgttg aaaaatatgg aaaagattac
    60061 gcaactactt caaaaaatac agtttactca ggaccatata ctgttgaagg ttggaatggt
    60121 tcgaatggta ctttcacgct gaagaaaaac aaaaattatt gggacgctaa aaatgtaaaa
    60181 acaaaagaag ttcgcatcca gactgttaaa aaaccagata ccgccgttca aatgtataaa
    60241 cgtggtgagt tagatgcagc taatatctca aatacttctg ctatttatca agctaataag
    60301 aataataaag atgtcacaga tgttctagaa gcgaccactg cctatatgga atataatact
    60361 actggttctg tgaaagggct tgataatgtt aagattcgtc gcgccttaaa cttagcaact
    60421 aaccgtaaag gagttgttca agcagccgtt gatacaggct caaaaccggc aattgctttt
    60481 gcacctactg gtttagccaa aacaccagat ggaactgatt tggcaaaata tgttgcccca
    60541 ggttatgaat ataataaaac tgaagcagca aaactcttta aagaaggttt ggctgaatca
    60601 ggcttgacta agctaaaatt aacaattaca gcagatgctg atgctcctgc tgccaaaaac
    60661 tctgttgact atatcaagtc tacttgggaa gctgctcttc caggacttac tgttgaagaa
    60721 aaatttgtaa cctttaaaca acgcttagaa gacagtagaa aacaaaactt tgacatcgta
    60781 gtttctcttt ggggtgggga ttatccagaa ggatcaactt tctacggcct ctttaagtca
    60841 gattcacaaa ataacgatgg aaaatttgct aacaaggact atgatgctgc ttataacaag
    60901 gcaatttctg aagatgcgat gaaaccagca gaatcagcga aggactataa agaagcagag
    60961 aaaattttat ttgagcaagg tgcttataac ccactctact tccgtagtgg taaaggttta
    61021 caaaatccaa aattaaaagg tgttattcgt aatactacag gtttgtcaat agactttaca
    61081 catgcttata aaaaataatt aaattacttt acactaaacc tttgcaagaa ttggtaattg
    61141 ccagttcttg cttttggtta agtaaagaaa gtgaaagagt ttgctatttt tagcaattct
    61201 aagaaaagga aatgatatga ttaaatatat tttaaagcgt gttgctattt tgttagtaac
    61261 gctatgggtt gtcataaccc tatcgttctt tctgatgcag atccttccag gaacacccta
    61321 caataatcca aaactcactg aagaaatgat cgcattgctt aataaacagt atggtttaga
    61381 caagcctgta tggcaacaat atttgactta cctttggaat gtacttcatg gtgactttgg
    61441 aacgagttat caatcagtga atcagccagt atcacgtatg attagcttga gattaggagt
    61501 ttcagtacat ctaggtgttc aggctctcgt atttggtgtt ttaggcggta ttttggtagg
    61561 agctatttcg gcacgtcata agaatgataa agtcgatggt atcttaagtg ttattgcaac
    61621 gctagggatt tctatgcctt catttatcat tggtatctta ttattagact attttggatt
    61681 taagtggaac ttgttgcctt tatcaggttg ggggactttc tctcaaacca ttttaccttc
    61741 tttagctcta ggactcccta cattagcatc tgtttcacgc ttcttccgta gtgaaatgat
    61801 tgaaacttta aattctgatt atgtgcaact agctcgttca aaaggtatga caattcgtca
    61861 agtaactcga aaacatgctt accgtaattc tatgattcca attttaactc taattggccc
    61921 tttggcggcg ggtctgttaa ctggttctgc actaattgag cagattttct cgattccagg
    61981 aattgggcag cagtttgtta cttcgattcc aacaaaagac tatcctgtta ttatggggac
    62041 aaccattgtt tatgcagtaa tgttgatggt tgctatcttg ataacagacg ttgttattag
    62101 tatcgtagac ccacgtgttc gcttgcagta aggagatgtt atggcagata aaaacagaac
    62161 atttaaactt gtaggtgcag gatcttctag cacacaagaa aaaattgaaa agcctgctct
    62221 ttcgtttatg caagatgcgt ggcgtcgctt gaaaaaaaac aaattagcag tagtttcact
    62281 ctatttatta gctcttttac ttactttttc gttagcctca aatttatttg taactcagaa
    62341 ggatgctaat gggtttgatt cgaaaaaagt aactacatat cgcaacttac cacctaaatt
    62401 gagttcaaac cttccttttt ggaatggtag cattaagtac gctggaaata cagaatctac
    62461 agacgcttat aagtctcaaa atgtccctga aaaagttaag tatgctttag gtacagattc
    62521 tctagggaga agtgttgcta agcgcatcat cgtaggtatt cggatttcct tacttgtagc
    62581 tattgcagct acattcattg atttaattat cggtgttaca tacggtcttg tttcaggttt
    62641 tgctggagga cgccttgata cattaatgca acgtatcgta gaggtaattt catcaattcc
    62701 aaacttagtt atcgttacaa tgttaggttt ggtccttggt aacggtatta cggctattat
    62761 tatctctatt gcttttaccg gatggacttc tatgtctcgt caggtaagga atttaaccct
    62821 atcatatcga gaaagagagt ttgtgcttgc tgcacgttca cttggggaaa gtccaattaa
    62881 aatagccttc aaacatattt tacctaacat ttctggtatt atcattgtac aaattatgat
    62941 gacaatccca agtgctatta tgtatgaagc ggtactttca gcaatcaatt taggtgttaa
    63001 accaccgaca gcttcattag gatcattgat ttcagatgca caagaaaatt tacaatatta
    63061 tccatatcaa gttatcttgc cagctttagc attggtaatg atttcattgg catttatttt
    63121 gcttggtgat gggttgcgag atgcttttga tccaaaatct agcgatgaat aaggaggtat
    63181 aatcatggaa aaagaaacta ttttaagtgt taataatctt catgttgact tccacacata
    63241 tgctggagaa gtaaaagcaa ttcgtgatgt caactttgaa ttaaaaaaag gtgagactct
    63301 tgcaatcgtt ggtgaatctg gttcaggaaa atctgtaact accagaactt taattggttt
    63361 aaatgctaaa aattcagaga tatcagggaa tgttcaattt aaggggcgta accttgttga
    63421 actatcagaa gaagagtgga ctaaggtacg cgggaatgag atttctatga ttttccaaga
    63481 ccctatgact agtttggatc caacgatgaa aattggcatg caaatagcag aaccaatgat
    63541 gattcatcaa aaaatttcaa aaaaggatgc tttaaaatta gctcttgagc taatgaaaga
    63601 tgtaggtatc ccaaatgctg aagagcatat taatgattac ccacatcagt ggtctggagg
    63661 aatgcgccaa cgtgcggtta tagcgattgc tttagctgct gatcctgaaa ttcttattgc
    63721 tgatgaacca acgactgctt tagatgtaac aattcaagca caaattttga acttaatgaa
    63781 aaaaattcaa gcagaacgag actcctccat tgttttcata actcatgact taggggtcgt
    63841 tgcagggatg gcagaccgtg tagcagttat gtatgcaggg aaaattgttg aatttggaac
    63901 tgttgatgaa gtcttttata atccacaaca tccatatact tggggattat tgaattcaat
    63961 gccgacaacc gacacagaat caggtagttt agagtcaatt ccgggaacac caccagattt
    64021 gttgaaccca cctaaaggag atgcatttgc agctcgtaat gaatttgcat tagatattga
    64081 tcatgaagaa gaaccaccgt acttcaaagt ttcagaaaca catttcgctg ctacatggct
    64141 tttagatgaa agatcaccta aggtgcttcc tccattaccg atccaaaaac gttgggaaaa
    64201 atggaacgag attgaaggga ggaaagccta atgactgaaa atcgaaaaaa attagttgaa
    64261 gtcaaaaatg tttctttgac cttcaataaa ggaaaagcta atgaagtgag agcaattgat
    64321 aatgttagtt ttgacattta tgaaggtgaa gtgtttggat tagttgggga atctggatca
    64381 ggaaagacaa ccgttggacg ttcaatttta aaactttatg atatttctga tggagaaatt
    64441 acctttaatg gagaagtgat ctcacatctg aaaggtaaag cattacatag ttttcgtaaa
    64501 gacgctcaga tgatttttca ggatcctcag gctagcttaa acggccgtat gaagattcgt
    64561 gatatcgtag cagaaggttt agatatacat aaattagcta aatcaaaatc agatcgtgat
    64621 agtaaagttc aggccctatt agatcttgta gggttaaata aagatcattt aacacgttat
    64681 ccgcatgaat tttcaggtgg acaacgacaa cgtatcggga ttgcacgtgc tttagcagta
    64741 gagcctaaat ttatcattgc tgatgaacca atatctgcct tagatgtttc aatccaagca
    64801 caagttgtta atttaatgca aaaattgcaa agagaacaag gattgactta tttgtttatc
    64861 gcacatgatt tgtctatggt aaaatacatt tcagatcgta tcggtgttat gcattggggg
    64921 aaactgttag aagttggaac atctgatgat gtttataaca atccaatcca cccttatacg
    64981 aagagtctat tatcagctat tccagaacca gatccggaga gtgaacgtca acgtgttcac
    65041 cagccgtata atccagctat tgagcaagac ggacaagaac gtcaaatgca cgagattacc
    65101 cctggtcatt ttgttttatc tacaccccaa gaagctgaag aatataaaaa acaaatatta
    65161 taaaagatgg ctagcccatc ttttattttt ttactaataa aataaattta ggtatggaat
    65221 tttaaactct atgttattgg tgttagccaa caggaaact
//
LOCUS       DATRMA010000004        50025 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.004, whole genome shotgun sequence.
ACCESSION   DATRMA010000004 DATRMA010000000
VERSION     DATRMA010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 50025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50025
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.004"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            complement(45..275)
                     /locus_tag="VBC28_000220"
     CDS             complement(45..275)
                     /locus_tag="VBC28_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1797 family protein"
                     /protein_id="HEO6814229.1"
                     /translation="MESHLVRIINRLELMATDGGNLKRNFEREGVVVAEVSFSNDPEN
                     GPVFTLRDVEARESYSFDSIDLIAMEIYDLLY"
     gene            573..2834
                     /locus_tag="VBC28_000221"
     CDS             573..2834
                     /locus_tag="VBC28_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEO6814230.1"
                     /translation="MLCQNCKLNESTIHLYTNVNGKQKQVDLCQNCYQIIKTDPNNPL
                     FSGLNHVSHAPGGINPFFDDFFGDLNNFRAFNGQDLPNTPPTQSGGNRGGGNGNGRNN
                     NRNQTATPSQAKGILEEFGINVTEIARHGDIDPVIGRDSEIIRVIEILNRRTKNNPVL
                     IGEPGVGKTAVVEGLAQKIVDGNVPHKLQGKQVIRLDVVSLVQGTGIRGQFEERMQKL
                     MEEIRQRQDVILFIDEIHEIVGAGTAGEGSMDAGNILKPALARGELQLVGATTLNEYR
                     IIEKDAALERRMQPVKVDEPSVEETITILKGIQKKYEDYHHVKYNNDAIEAAAVLSNR
                     YIQDRFLPDKAIDLLDEAGSKMNLTLNFVDPKEIDQRLIEAENLKAQATREEDYERAA
                     YFRDQIAKYKEMQQQKVDDQDTPIITEKTIEHIIEEKTNIPVGDLKEKEQSQLINLAD
                     DLKQHVIGQDDAVVKIAKAIRRNRVGLGSPNRPIGSFLFVGPTGVGKTELSKQLAIEL
                     FGSADSMIRFDMSEYMEKHAVAKLVGAPPGYVGYEEAGQLTEKVRRNPYSLILLDEIE
                     KAHPDVMHMFLQVLDDGRLTDGQGRTVSFKDTIIIMTSNAGSGKTEASVGFGASREGR
                     TNSVLGQLGNFFSPEFMNRFDGIIEFKALDKENLLNIVDIMLSDVNARLAINGIHLDV
                     TDKVKEKLVDLGYDPKMGARPLRRTIQEHIEDAITDYYLENPSEKELRAIMTSNGNII
                     IKSSKKTEESTKG"
     gene            3020..3475
                     /locus_tag="VBC28_000222"
     CDS             3020..3475
                     /locus_tag="VBC28_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEO6814231.1"
                     /translation="MTNPTFGEKIDNVNYRSRFGVYAIIPNPTHDKIILVQAPNGAWF
                     LPGGEIEENENHLEALTRELIEELGYSATIGHYYGQADEYFYSRHRDTYYYNPAYIYE
                     VTAYHKDQAPLEDFNHLAWFPIQEAKEKLKRGSHRWGVQAWEKNHHSRK"
     gene            3539..3841
                     /locus_tag="VBC28_000223"
     CDS             3539..3841
                     /locus_tag="VBC28_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HEO6814232.1"
                     /translation="MRLINTTSSHPELVRNQLQNTDAKLVEVYSAGNTDVVFTKAPKH
                     YELLISNKYRAIKDEELEAIREFFLKRKIDQSIIIQEQMKSLHTAKLIEISYPTTA"
     gene            complement(3958..6750)
                     /locus_tag="VBC28_000224"
     CDS             complement(3958..6750)
                     /locus_tag="VBC28_000224"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEO6814233.1"
                     /translation="MKLKETLNLGQTAFPMRAGLPNKEPQWQEAWDQADIYKKRQALN
                     EGKPAFHLHDGPPYANGNIHVGHALNKISKDIIVRSKSMSGFRAPYVPGWDTHGLPIE
                     QVLAKKGVKRKEMDLAEYLEMCRDYALSQVDKQRDDFKRLGVSADWENPYITLTPDYE
                     ADQVRVFGAMADKGYIYRGAKPVYWSWSSESALAEAEIEYHDIDSTSLYYANKVKDGK
                     GILDTDTYIVVWTTTPFTVTASRGLTVGPDMEYVVVVPVGSERKYLLAEVLVDSLAAK
                     FGWENFEIVTHHTGKELNHIVTEHPWDTEVEELVILGDHVTTDSGTGIVHTAPGFGED
                     DYNVGIANGLDVVVTVDSRGLMMENAGPDFEGQFYDKVTPLVKEKLGDLLLASEVINH
                     SYPFDWRTKKPIIWRAVPQWFASVSKFRQEILDEIEKTNFQPEWGKKRLYNMIRDRGD
                     WVISRQRAWGVPLPIFYAEDGTAIMTKEVTDHVADLFAEYGSIVWWQRDAKDLLPAGY
                     THPGSPNGLFEKETDIMDVWFDSGSSWNGVMNARENLSYPADLYLEGSDQYRGWFNSS
                     LITSVAVNGHAPYKAVLSQGFVLDGKGEKMSKSLGNTILPSDVEKQFGAEILRLWVTS
                     VDSSNDVRISMDILKQTSETYRKIRNTLRFLIANTSDFNPKQDAVAYENLGAVDRYMT
                     IKFNQVVDTINKAYAAYDFMAIYKAVVNFVTVDLSAFYLDFAKDVVYIEAANSPERRR
                     MQTVFYDILVKLTKLLTPILPHTAEEIWSYLEHEEEEFVQLAEMPVAQTFSGQEEILE
                     EWSAFMTLRTQAQKALEEARNAKVIGKSLEAHLTIYASQEVKTLLTALNSDIALLMIV
                     SQLTIADEADKPADSVSFEGVAFTVEHAEGEVCERSRRIDPTTKMRSYGVAVCDASAA
                     IIEQYYPEAVAQGFEA"
     gene            complement(7035..7805)
                     /locus_tag="VBC28_000225"
     CDS             complement(7035..7805)
                     /locus_tag="VBC28_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HEO6814234.1"
                     /translation="MPLTALEIKDKTFSSKFRGYSEEEVNEFLEIVVDDYEDLIRRNR
                     EQEQYIKDLEEKIAYFNEMKESLSQSVILAQETAERVKISAQDEASNLMGKATFDAQH
                     LIDEAKLKANQILRDATDDAKRVAIETEDLKRQSRVFHQRLLSELEGQLKLANSSAWE
                     ELLKPTAIYLQNSDASFKEVVEKVLDEDDALPVVDDTESFDATRQFSPDEMEELQRRV
                     EESNKQLEESGLLDTNNFQMEEPINLGETQTFKLNIED"
     gene            complement(7815..8603)
                     /locus_tag="VBC28_000226"
     CDS             complement(7815..8603)
                     /locus_tag="VBC28_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEO6814235.1"
                     /translation="MTLDDIYQHFRPEEYAFIHKIDHLAQYVENTYSFITTEFLNPRE
                     FKILESVLERRGSHYYTSGQYFQTEYVKVIIAPEYYQLDMADFNLSLIEIKYNAKFNH
                     LTHAKIMGTLLNYLGVKRSILGDILVEEGCAQVLVDSQMTNHLVHSVTKIGTASVQLA
                     EVPLSKLLTPKQDIQKLTVIASSLRLDKILATILKISRTQSTKLIEADKVKVNYATVN
                     RVSEQLVEGDLISVRGYGRFTLNHNLGLTKNQKYKLEVDKMIHN"
     gene            complement(8605..8859)
                     /locus_tag="VBC28_000227"
     CDS             complement(8605..8859)
                     /locus_tag="VBC28_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEO6814236.1"
                     /translation="MILVLLILLKTIRVYSYILLAYALLSWFPGAYDSWIGRLVVQIV
                     DPIIRPLRRFNLQFLGLDFTVIVAFFLLNMLSRFLILLFT"
     gene            complement(8862..9467)
                     /locus_tag="VBC28_000228"
     CDS             complement(8862..9467)
                     /locus_tag="VBC28_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001185364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HEO6814237.1"
                     /translation="MALKDRFDKIISYFDTDDVSENEVHEVQERTSVQRDSRAATAQE
                     ASQRSHMTNSAEEEMIGSRPRTYTYDPNRQERQRVQRDNAYQQATPRVQNKDSVRQQR
                     EQVTIALKYPRKYEDAQEIVDLLIVNECVLIDFQYMLDAQARRCLDYIDGASRVLYGS
                     LQKVGSSMFLLTPANVMVDIEEMNIPKTGQETSFDFDMKRR"
     gene            complement(9479..10153)
                     /locus_tag="VBC28_000229"
     CDS             complement(9479..10153)
                     /locus_tag="VBC28_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000981484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEO6814238.1"
                     /translation="MMNLQENKTAIFDNVSKLALKAGRAHESVHIVAVTKYVNCQTTE
                     ALIRTGVNHIGENRVDKFLEKYQALKDEKLTWHLIGSLQRRKVKDVINYVDYFHALDS
                     VKLAAEIQKRAQKPIKCFLQVNISREDSKHGFTIEQIDDALNLISRYDKIELIGIMTM
                     APLKATKEEISSIFEETESLRKRLQARNIERMPFTELSMGMSRDYDIAIQNGSTFVRI
                     GTSFFK"
     gene            complement(10159..11439)
                     /gene="ftsZ"
                     /locus_tag="VBC28_000230"
     CDS             complement(10159..11439)
                     /gene="ftsZ"
                     /locus_tag="VBC28_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEO6814239.1"
                     /translation="MVFSFDTASVQGAVIKVIGVGGGGGNAINRMIDEGVAGVEFIAA
                     NTDIQALSSSKAETVIQLGPKLTRGLGAGGQPEVGRKAAEESEEVLTEALTGADMVFI
                     TAGMGGGSGTGAAPVIARIAKSLGALTVAVITRPFGFEGNKRSNFAIEGIQELREQVD
                     TLLIISNNNLLEIVDKKTPLLEALSEADNVLRQGVQGITDLITNPGLINLDFADVKTV
                     MANKGNALMGIGIGSGEERITEAARKAIYSPLLETTIDGAEDVIVNVTGGMDMTLTEA
                     EEASEIVSQAAGKGVNIWLGTSIDMDMKDEIRVTVVATGVRKDKTNQVSGFTTSAPTN
                     QAPSERQSTSNSNFDRRGNFDMTESREMPTQQNQPHAQNQQQSSAFGNWDLRRDNISR
                     PTEGELDSKLSMSTFSENDDMDDELETPPFFKNR"
     gene            complement(11461..12834)
                     /gene="ftsA"
                     /locus_tag="VBC28_000231"
     CDS             complement(11461..12834)
                     /gene="ftsA"
                     /locus_tag="VBC28_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEO6814240.1"
                     /translation="MARNGFFTGLDIGTSSIKVLVAEFIANEMNVIGVSNVPSSGVKD
                     GIIIDIEAAATAIKEAVKQAEEKAGITIDKINVGLPANLLQIEPTQGMIPVPNESKEI
                     KDEDVESVVKSALTKSITPEREVISLIPLEFIVDGFQGIRDPRGMMGIRLEMRGLIYT
                     GPTTILHNLRKTVERAGIKVEHVVIAPLALAKSVLNEGEREFGATVIDMGGGQTTVAS
                     MRNQELQYTNIYSEGSDYVTKDISKVLRTTVEIAEALKFNFGQANVEEASTSDTVQVN
                     VVGNEEPVEITESYLSQIISGRIRQILEHVKQDLGRGRLLDLPGGIILVGGGAIMPGV
                     VEVAQQIFGTRVKLHVPNQVGIRNPMFANVISIVDYVGMMSEVDIIAQHAVTGDEMLR
                     HKPVDFDYKEKTNTMSTMPYSEPLTSSMEDSNLEPIRARENAQEPTEPKANIGERIRG
                     IFGSMFD"
     gene            complement(13106..14242)
                     /locus_tag="VBC28_000232"
     CDS             complement(13106..14242)
                     /locus_tag="VBC28_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001124859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HEO6814241.1"
                     /translation="MPKKKSDTPEKEEVVLTEWQKRNLEFLKKRKEDEEEQKRINEKL
                     RLDKRSKLNISSPEEPQNTTKIKKLHFPKISRPKIEKKQKKEKIVNSLAKTNRIRTAP
                     IFVVAFLVILVSVFLLTPFSKQKTITVSGNQHTPDDILIEKTNIQKNDYFFSLIFKHK
                     AIEQRLAAEDVWVKTAQMTYQFPNKFHIQVQENKIIAYAHTKQGYQPVLETGKKADPV
                     NSSELPKHFLTINLDKEDSIKLLIKDLKALDPDLISEIQVISLADSKTTPDLLLLDMH
                     DGNSIRIPLSKFKERLPFYKQIKKNLKEPSIVDMEVGVYTTTNTIESTPVKAEDTKNK
                     STDKTQTQNGQVAENSQGQTNNSNTNQQGQQIATEQAPNPQNVN"
     gene            complement(14246..15322)
                     /locus_tag="VBC28_000233"
     CDS             complement(14246..15322)
                     /locus_tag="VBC28_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HEO6814242.1"
                     /translation="MGKKIVFTGGGTVGHVTLNLILIPKFIKDGWEVHYIGDKNGIEH
                     EQINQSGLDITFHSIATGKLRRYFSWQNMLDVFKVGVGVLQSIAIIAKLRPQALFSKG
                     GFVSVPPVVAARLLKVPVFVHESDLSMGLANKIAYKFATIMYTTFEQSKDLIKTKHIG
                     AVTKVMDCKKSFENTDLTSIKEAFDPNLKTLLFIGGSAGAKVFNDFITQTPELEEKYN
                     VINISGDSSLNRLKKNLYRVDYVTDLYQPLMNLADVVVTRGGSNTIFELVAMKKLHLI
                     IPLGREASRGDQLENAAYFEEKGYALQLPESELNINTLEKQINLLISNSESYEKNMSQ
                     SSEIKSQDEFYQLLIDDMAKVTKG"
     gene            complement(15325..16680)
                     /locus_tag="VBC28_000234"
     CDS             complement(15325..16680)
                     /locus_tag="VBC28_000234"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEO6814243.1"
                     /translation="MKTITTFENKKVLVLGLARSGEAAARLLAKLGAIVTVNDGKPFD
                     ENPTAQSLLEEGIKVVCGSHPLELLDEDFCYMIKNPGIPYNNPMVKKALEKQIPVLTE
                     VELAYLVSESQLIGITGSNGKTTTTTMIAEVLNAGGQRGLLAGNIGFPASEVVQAAND
                     KDTLVMELSSFQLMGVKEFRPHIAVITNLMPTHLDYHGSFEDYVAAKWNIQNQMSSSD
                     FLVLNFNQGISKELAKTTKATIVPFSTTEKVDGAYVQDKQLFYKGENIMSVDDIGVPG
                     SHNVENALATIAVAKLAGISNQVIRETLSNFGGVKHRLQSLGKVHGISFYNDSKSTNI
                     LATQKALSGFDNTKVILIAGGLDRGNEFDELIPDITGLKHMVVLGESASRVKRAAQKA
                     GVTYSDALDVRDAVHKAYEVAQQGDVILLSPANASWDMYKNFEVRGDEFIDTFESLRG
                     E"
     gene            complement(16810..17055)
                     /locus_tag="VBC28_000235"
     CDS             complement(16810..17055)
                     /locus_tag="VBC28_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3165 family protein"
                     /protein_id="HEO6814244.1"
                     /translation="MFYLIVALLIALYYFFMAPKTVRNTLNAIGLVGLVALLLVLAVM
                     SFIKILQLPGELYIGLIMIPLGYTAFKETLNLSEKKK"
     gene            complement(17100..18941)
                     /gene="typA"
                     /locus_tag="VBC28_000236"
     CDS             complement(17100..18941)
                     /gene="typA"
                     /locus_tag="VBC28_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEO6814245.1"
                     /translation="MTNLRTDIRNVAIIAHVDHGKTTLVDELLKQSHTLDERKELEER
                     AMDSNDIEKERGITILAKNTAVAYNDVRINIMDTPGHADFGGEVERIMKMVDGVVLVV
                     DAYEGTMPQTRFVLKKALEQNLIPIVVVNKIDKPSARPSEVVDEVLELFIELGADDDQ
                     LDFPVVYASAINGTSSMSDDPSDQEKTMAPIFDTIIDHIPAPVDNSEEPLQFQVSLLD
                     YNDFVGRIGIGRVFRGTVKVGDQVTLSKLDGTTKNFRVTKLFGFFGLERKEIQEAKAG
                     DLIAVSGMEDIFVGETVTPTDAIEPLPVLRIDEPTLQMTFLVNNSPFAGREGKWITSR
                     KVEERLLAELQTDVSLRVDPTDSPDKWTVSGRGELHLSILIETMRREGYELQVSRPEV
                     IIKEIDGVQCEPFERVQIDTPEEYQGAIIQSLSERKGDMLDMQMVGNGQTRLIFLIPA
                     RGLIGYSTEFLSMTRGYGIMNHTFDQYLPVVQGEIGGRHRGALVSIENGKATTYSIMR
                     IEERGTIFVNPGIEVYEGMIVGENSRDNDLGVNITTAKQMTNVRSATKDQTAVIKTPR
                     ILTLEESLEFLADDEYMEVTPESIRLRKQILNKAARDKANKKKKSAE"
     gene            complement(19173..19553)
                     /locus_tag="VBC28_000237"
     CDS             complement(19173..19553)
                     /locus_tag="VBC28_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEO6814246.1"
                     /translation="MDMSVILIIVILLAFVAWASWNYWRVRRAAKFLDNESFQKEMSR
                     GQLIDIREAGAFHRKHILGARNIPASQFKVALSALRKDKPVLLYDASRGQSIPRIVLL
                     LRKEGFNQLYVLKDGFNYWTGRVK"
     gene            complement(19565..20533)
                     /locus_tag="VBC28_000238"
     CDS             complement(19565..20533)
                     /locus_tag="VBC28_000238"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HEO6814247.1"
                     /translation="MSKKLLGIDLGGTTIKFGILTLEGEVQEKWAIETNTLENGRHIV
                     SDIVESLKHRLSLYGLTKDDFLGIGMGSPGAVDRTSKTVTGAFNLNWADTQEVGSVIE
                     KEVGIPFFIDNDANVAALGERWVGAGANNPDVVFVTLGTGVGGGVIADGNLIHGVAGA
                     GGEIGHMIVDPENGFTCTCGNKGCLETVASATGVVRVARQLAEQYEGSSAIKAAIDNG
                     DTVTSKDIFIAAEDGDKFANSVVERVSRYLGLAAANISNILNPDSVVIGGGVSAAGEF
                     LRSRVEKYFVTFAFPQVKKSTKIKIAELGNDAGIIGAASLANQQAS"
     gene            complement(20530..20739)
                     /locus_tag="VBC28_000239"
     CDS             complement(20530..20739)
                     /locus_tag="VBC28_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HEO6814248.1"
                     /translation="MKVLFDVQNLLKKFGIYVYIGKRLYDIEVMKIELQRLYDNGLIS
                     RDDYLKAELILRREHRLELEKENKK"
     gene            20894..21289
                     /locus_tag="VBC28_000240"
     CDS             20894..21289
                     /locus_tag="VBC28_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001884316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEO6814249.1"
                     /translation="MIMSTIYFISLCMSFILSYYDIKYQEYPIFLWILFTISTIILTP
                     ITKVSIVLCLFGILAEVVDINIGSGDFLYLATIGLSLPLHQMLFIIQIGAWLGIIYCL
                     VMRKMKKTIAFLPFLSIAYIIVTSYSLLF"
     gene            complement(21290..21922)
                     /gene="nth"
                     /locus_tag="VBC28_000241"
     CDS             complement(21290..21922)
                     /gene="nth"
                     /locus_tag="VBC28_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEO6814250.1"
                     /translation="MLSKAKSRYIIREIIKLFPDAKPSLDFTNVFELLVAVMLSAQTT
                     DAAVNKVTPALFERFPNPLVLAQADPKEIEPYISKIGLYRNKARFLNQCAKQLIEHFD
                     GKVPRTRQELESLAGVGRKTANVVMSVGFGIPAFAVDTHVTRICKHHQICKQSASPLE
                     IEKRVMEVLPPEEWLAAHQSMIYFGRAICHPKNPKCDQYPQLYHFPDNLK"
     gene            complement(22035..23264)
                     /locus_tag="VBC28_000242"
     CDS             complement(22035..23264)
                     /locus_tag="VBC28_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEO6814251.1"
                     /translation="MLESLKTIVKTNSDKKLFDGDLQVSYGEFYNLVRQDMASQDNRK
                     HVISTHSLLNQLVRFVSKLCQKALPIICKPNLTHNEISRLEKEVQYAPQLADFGVLSS
                     GTTADAKLLWRSFTSWSDFFSIQNAYFSVTSNSKLFIQGDFSFTGNLNLALSLLLLGG
                     TLVVTQKNSVKYWQTLWEKTGVTHLYLLPSYLKLVEQYSKETALDNKTIITSSQYVSD
                     SLLEGLYRKHPKVSVKIFYGASELNYVSWYDGRDIRDKPQYVGEIVPNVAVRIKEGRI
                     FVKTPYSICGLSSEYCAGDYGELIDGKLYLFGRGGDWCNQSGIKLYLPRLIEKIKTCP
                     YIKDAVAFTKESQSHGQESHCCIVLIENQMQQECLKWLSEHFEKKYGFKHYHIVSKIP
                     LMPSGKIDYQQLKRQLA"
     gene            complement(23257..24372)
                     /locus_tag="VBC28_000243"
     CDS             complement(23257..24372)
                     /locus_tag="VBC28_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000796101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEO6814252.1"
                     /translation="MKNRDVYIGFGLRTPIGIKGKQFKHYRPELLGAHLLNQIKKIES
                     ESNIDSIICGNTVGTGGNIGRLMTLFSDYESYIPVQTIDMQCASSSSALFFGYLKIST
                     GINEKVLVGGIESSSLQPMRRYAKEDNRNGEYTVAQFSPDSYAETVMLEGAQRVCQKY
                     GFRREMLDKLAFLSHKRALTAKQGGYLEEVILPMEGMRDQGVRKLKETFFQKLPRLME
                     NSPLLTIGNVCLMHDAAAFLTLQSQKTEFRIVHIVEVAGDPKLSPELVHTATEKLLTE
                     THTKISDYDAIEWNEPFAAIDALFNHYYPEEREKFNIFGGTLAYGHPYACSGIINILH
                     LMQALKYKNKPMGLTAIAGAGGVGMAISIEYLGVKNA"
     gene            complement(24382..24876)
                     /locus_tag="VBC28_000244"
     CDS             complement(24382..24876)
                     /locus_tag="VBC28_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000732601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6814253.1"
                     /translation="MKKLFKKAIAMCRDLPLNFVIVELSLYQREDGSSDNIKWISKRI
                     LEVTVTDQQWIYHLRMLNLPKLKPMSLASSDCPAREEKASLISIEECQKFTSLVRDKN
                     PFHQGEAAIVPAALLLEKIMEVRKGKIKEIHLTFLSPVRVNQEFSVQVVDNTVIIFQH
                     QILW"
     gene            25001..25993
                     /gene="bioB"
                     /locus_tag="VBC28_000245"
     CDS             25001..25993
                     /gene="bioB"
                     /locus_tag="VBC28_000245"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000009960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HEO6814254.1"
                     /translation="MSFQTNYIHLADEILSGKTSISYEQALEILNSDENWWEIYAAAL
                     YLKNQVSRNNIRLNVLLSAKQGLCAENCGYCSQSKESTADIDKFGLLPQNVILKQAIV
                     AHQNGASVFCIAMSGTKPSKREIEQLCQVIPEIKKSLPLEICLTAGFLDREQLHQLKQ
                     AGIDRINHNLNTPEENYPNIATTHSFKDRCDTLERIHNEDIDVCSGFICGMGESDEGL
                     ITLAFRLKELDPYSIPVNFLLAVEGTPLGKYNYLTPIKCLKIMAMLRFVFPFKELRLS
                     AGREVHFENFESLVTLLVDSTFLGNYLTEGGRNQHTDIEFLEKLQLNHTKKELI"
     gene            25994..26533
                     /locus_tag="VBC28_000246"
     CDS             25994..26533
                     /locus_tag="VBC28_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HEO6814255.1"
                     /translation="MKTRTTTYIALMVALLIVLGFIPGIPLGFIPVPIVLQNLGVMLA
                     GALLGSRKGFLAVAIFLLLVAIGAPFLPGGRSGLVTLFGPTAGYLLTYPFAAFFIGLG
                     LEKVKTTKLWVQFLIIWIFGVLLIDICGSIVLSFQTTLPLTKSLFSNLIFIPGDTLKA
                     SICLIIYRKFANRLTHLYN"
     gene            complement(26600..27166)
                     /locus_tag="VBC28_000247"
     CDS             complement(26600..27166)
                     /locus_tag="VBC28_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEO6814256.1"
                     /translation="MLLLVDNYDSFTYNLKQYLSVYKEVFVIKNDVPNLFLLAESAEA
                     IVLSPGPGHPKDAGKMVELINQFIGKKPILGICLGHQALAECLGGRLNLANHVMHGKQ
                     SWVTINDHTSLFKGIDSPTQVMRYHSLVVTDLPENIAVIARSNEDNEIMAFHCPSLKV
                     YAMQFHPESIGSIDGMKMIENFLTLIND"
     gene            complement(27294..29033)
                     /locus_tag="VBC28_000248"
     CDS             complement(27294..29033)
                     /locus_tag="VBC28_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6814257.1"
                     /translation="MKSNQWQVFKRLISYLRPYKWFTVLALSLLLLTTVVKNIIPLIA
                     SHFIDHYLTNVNQTAVLILVGYYSMYVLQTLIQYFGNLFFARVSYSIVRDIRRDAFAN
                     MERLGMSYFDRTPAGSIVSRITNDTEAISDMFSGILSSFISAIFIFTVTLYTMLMLDI
                     KLTGLVALLLPVIFILVNVYRKKSVTVIAKTRSLLSDINSKLSESIEGIRIVQAFGQE
                     ERLKTEFEEINKEHVVYANRSMALDSLFLRPAMSLLKLLAYAVLMAYFGFTGVKGGLT
                     AGLMYAFIQYVNRLFDPLIEVTQNFSTLQTSMVSAGRVFDLIDETGFEPSQKNTEAFV
                     REGNIEFKNVSFSYDGKKQILDNVSFSVKKGETIAFVGATGSGKSSIINVFMRFYEFQ
                     SGQVLLDGKDIRDYSQEQLRKNIGLVLQDPFLYHGTIKSNIKMYQDITDQEVQDAAEF
                     VDADQFIQKLPDKYDAAVSERGSSFSTGQRQLLAFARTVASKPKILILDEATANIDSE
                     TEQIVQDSLAKMRQGRTTIAIAHRLSTIQDANCIYVLDRGKIIESGNHESLLDLKGTY
                     YRMYQLQAGMMEV"
     gene            complement(29023..30768)
                     /locus_tag="VBC28_000249"
     CDS             complement(29023..30768)
                     /locus_tag="VBC28_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO6814258.1"
                     /translation="MSIIKNLWWFFKEEKKRYLIGILSLSLVAVLNLIPPKIMGSVID
                     AITTGKLTRPQLLWNLLGLVLSALAMYGLRYIWRMYILGTSYKLGQVVRYRLFEHFTK
                     MSPSFYQKYRTGDLMAHATNDINSLTRLAGGGVMSAVDASITALVTLITMFFTISWQM
                     TLIAVIPLPLMALATSKLGRKTHETFKESQAAFSELNNKVQESVSGVKVTKSFGYQEQ
                     EIASFQEVNQMTFVKNMRTMTYDVMFDPLVLLFIGASYVLTLAMGAFMISKGQVTVGD
                     LVTFVTYLDMLVWPLMAIGFLFNMVQRGSVSYNRINSLLEQESDITDPLNPIRPVVNG
                     TLRYDIDFFRYDNEETLADIHFTLEKGQTLGLVGQTGSGKTSLIKLLLREHDVTQGKI
                     TLNKHDIRDYRLSELRQLIGYVPQDQFLFATSILENVRFGNPTLSINAVKKATKLAHV
                     YDDIKQMPAGFETLIGEKGVSLSGGQKQRIAMSRAMILDPDILILDDSLSAVDAKTEH
                     AIIENLKTNRQGKSTIISAHRLSAVVHADLILVMQDGRVIERGQHQELLNKGGWYAET
                     YASQRLEMEEAFDEV"
     gene            complement(30947..31462)
                     /locus_tag="VBC28_000250"
     CDS             complement(30947..31462)
                     /locus_tag="VBC28_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEO6814259.1"
                     /translation="MLKRLLTEDGELTKISRRFVWMLVIIYCLIIVRMCFGPQIMIEG
                     VSTPNVQRFGRIVALLVPFNSFRSLDQLTSFKEIFWVIGQNVVNILLLFPLIIGLLSL
                     KPSLRKYKSVILLAFLMSIFIECTQVVLDILIDANRVFEIDDLWTNTLGGPFALWTYR
                     NIKGWLLTIRK"
     gene            complement(31462..32568)
                     /gene="rlmN"
                     /locus_tag="VBC28_000251"
     CDS             complement(31462..32568)
                     /gene="rlmN"
                     /locus_tag="VBC28_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001124995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEO6814260.1"
                     /translation="MPKKTDTPAYKPSIYSLTRDELIAWAIEHGEKKFRASQIWDWLY
                     KKRVQSFDEMTNISKDFIALLNENFVVNPLKQRIVQESADGTVKYLFELPDGMLIETV
                     LMRQHYGLSVCVTTQVGCNIGCTFCASGLIKKQRDLNNGEITAQIMLVQKYFDERGQG
                     ERVSHIVVMGIGEPFDNYTNVLKFLRTVNDDNGLAIGARHITVSTSGLAHKIREFANE
                     GVQVNLAVSLHAPNNDLRSSIMRINRSFPLEKLFAAIEYYIETTNRRVTFEYIMLNGV
                     NDTPENAQELADLTKKIRKLSYVNLIPYNPVSEHDQYSRSPKERVEAFYDVLKKNGVN
                     CVVRQEHGTDIDAACGQLRSNTMKRDRQKAKVGQ"
     gene            complement(32619..33197)
                     /locus_tag="VBC28_000252"
     CDS             complement(32619..33197)
                     /locus_tag="VBC28_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1027 domain-containing protein"
                     /protein_id="HEO6814261.1"
                     /translation="MRKEVTPEMLNYNKYPGPQFIHFENIVKSDDIEFQLVINEKSAF
                     DVTVFGQRFSEILLKYDFIVGDWGNEQLRLRGFYKDASTIRKNSRISRLEDYIKEYCN
                     FGCAYFVLENPNPRDIKFDDERPHKRRKSRSKSQSSKSQTRNNRSQSNANAHFTSKKR
                     KDTKRRQERHIKEEQDKEMTSAKQHFVIRKKK"
     gene            complement(33266..34598)
                     /locus_tag="VBC28_000253"
                     /pseudo
     CDS             complement(33266..34598)
                     /locus_tag="VBC28_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644500.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
     gene            complement(34673..35746)
                     /locus_tag="VBC28_000254"
     CDS             complement(34673..35746)
                     /locus_tag="VBC28_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEO6814262.1"
                     /translation="MKNRDPKRKHKSLLGLLKWWIIGFAFLLLVLASLVVRLPYYLEM
                     PGGAYDIRSVLKVNKKADKAKGSYNFVAVSVSQATPAQVLYAWLTPFTELSSKEETTG
                     GFSNDDYLRINQFYMETSQNESIYQALKLANKQVSLTYKGVYVLNLAKNSTFKDRLHL
                     ADTVTGVNGKSFKNSSQLIKYVAALHLGDKVKVQYTSQGKKKESVGKVIKLSNGKNGI
                     GIGLTDHTEVLSDVPVDFNTEGVGGPSAGLMFTLAIYDQLVKEDLRKGRKIAGTGTIE
                     QNGHVGDIGGAGLKVVSAAKKGMDIFFVPNNPIDKNAKKGKTKVQTNYQEAKAAAKRL
                     GTKMKIVPVQNVQQAIDYLKKTK"
     gene            complement(35736..36221)
                     /gene="coaD"
                     /locus_tag="VBC28_000255"
     CDS             complement(35736..36221)
                     /gene="coaD"
                     /locus_tag="VBC28_000255"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEO6814263.1"
                     /translation="MTKKALFTGSFDPVTNGHLDIIERASYLFDHVYIGLFYNLEKQG
                     YFSIECRKKMLEEAIRQFKNVSVLVAQDRLAVDLAREVGAKYFVRGLRNSQDFDYEAN
                     LEFFNKQLADDIETVYLSTSPSLSPISSSRIRELIHFKASVKPFVPKSVVREVEKMSE
                     E"
     gene            complement(36218..36508)
                     /locus_tag="VBC28_000256"
     CDS             complement(36218..36508)
                     /locus_tag="VBC28_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000384890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6814264.1"
                     /translation="MDYKLVMIILILVILIYLLLRLHYLEELLKHYHFKNDEGVCSLS
                     RTKNPLYLLMAMITFLPNLINWIANGFEAIDIVRLIPVVVFLWLYYCGDDDE"
     gene            complement(36520..37059)
                     /gene="rsmD"
                     /locus_tag="VBC28_000257"
     CDS             complement(36520..37059)
                     /gene="rsmD"
                     /locus_tag="VBC28_000257"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEO6814265.1"
                     /translation="MRVVAGTFGGRPLKTLDGKTTRPTTDKVKGAIFNMIGPFFEGGR
                     VLDLFSGSGSLAIEAISRGMDQAVLVEKDRRAQVVIQENIAMTKSPEQFQLLKMEANR
                     ALEQLTGQFDLVLLDPPYAKEEIVKQIQIMDSKGLLGDDIMIACETDKSVDLPEEIAS
                     FGIWKQKIYGISKVTVYVR"
     gene            37189..37638
                     /locus_tag="VBC28_000258"
     CDS             37189..37638
                     /locus_tag="VBC28_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001893380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductase thioredoxin"
                     /protein_id="HEO6814266.1"
                     /translation="MIKKLLLTLIAILCLTTLKSNLSSADTMTISQQQYQHMITNSDL
                     IAITDKALVSKSQQSEATIFIGRPTCQYCRAFLPKLLKSQATLHSKIYYLDSQKYKGK
                     RLKSFFKKHHITTVPNLAHYQQGKMTKYLVQGSQATPQQIQTFLSQK"
     gene            complement(37683..38675)
                     /locus_tag="VBC28_000259"
     CDS             complement(37683..38675)
                     /locus_tag="VBC28_000259"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HEO6814267.1"
                     /translation="MKKSFIHQQQEISFVKNTFTQYLIDKLEIVEVQGPILSQVGDGM
                     QDNLSGIEHPVSVKVLNIPEAEFEVVHSLAKWKRHTLARFGFNEGEGLFVHMKALRPD
                     EDSLDPTHSVYVDQWDWEKVIPDGRRNLDYLKETVEKIYKAIRLTELAVEARFDIESI
                     LPKRITFIHTEELVEKYPDLSPKERENAIAKEYGAVFLIGIGGELADGKPHDGRAPDY
                     DDWTTPSENGFKGLNGDILVWNEQLGTAFELSSMGIRVDEDALKRQVVLTGDEDRLEF
                     EWHKTLLRGFFPLTIGGGIGQSRLAMFLLRKKHIGEVQSSVWPKEVRDTFENIL"
     gene            complement(38789..39853)
                     /locus_tag="VBC28_000260"
     CDS             complement(38789..39853)
                     /locus_tag="VBC28_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEO6814268.1"
                     /translation="MSIESDFFRKKRFIFSSLEEFGFIKSDQEYIYCQTFMDNDFKAI
                     ITISLDGKIAGKVIDSALEEEYLPLRAANYNGSFVGEVRSAYMAILGDISDSCCKDLL
                     FTKDQSNRLAEKIAKTFEDSVDYPFAKHPQYASYRVSGKWYALLFPLKMGKLENVPAQ
                     LSEDEVEVLNIKVNPQDMEILLQKEGIYPSYHMSKKTWVSIVLDNTLSDIEIFKLVSD
                     SRKLVSHNKKSNSEPEFWIIPANPKFYDIDKEFAEHQLIDWTQKGKIAVGDYVFIYIT
                     APIRALRYVCQVIERDLKTSTYLGARDVKAYMRLKLIRQFEDSTYPVAFLAEHGVKTV
                     RGPRRMTKELRQVMAKTILE"
     gene            40159..41334
                     /locus_tag="VBC28_000261"
     CDS             40159..41334
                     /locus_tag="VBC28_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000191693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEO6814269.1"
                     /translation="MTQFTTELLNFLAQKQDIDEFFRSSLETAMNDLLQVELSAFLGY
                     EPYDKAGYNTGNSRNGAYTRRFETKYGVVNLLIPRDRNGEFSPALIPSYGRRDNHLEE
                     MVIKLYRTGVTTREISDIIERMYGHHYSPATVSNISKATQENVASFHERSLEANYTVL
                     YLDGTYLPLRRGTVSKECIHIALGVTSYGHKAILGYDIAPNENNASWSDLLERFKGQG
                     VQQVSLVVSDGFNGLDQLIQQAFPMAKQQRCLVHIGRNIASKVKRADRALILEQFKTI
                     YRAINVEEAKQALDSFINEWKPHYKKVIETLESIENLLIFYEFPHQIWGSIYSTNLIE
                     SLNKEIKRQTKKKVVFPNEESLERYLVTLFSDYNFKQGQRIHKGFGQCTDTLESLFD"
     gene            complement(41381..42244)
                     /locus_tag="VBC28_000262"
     CDS             complement(41381..42244)
                     /locus_tag="VBC28_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000492950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEO6814270.1"
                     /translation="MFSSDFGFEIINVDDLSLQEERFLPSELLAYARDENESSFVRDI
                     EGHLALVYQLLDTQGHVDDVRHVPRVIPVTLFLKEDGLFVLANHKNINLVKKALNRVE
                     KVDSPKHLLLSLVTAFSKQYFDVLDTISEERDKLINDLRKRPNKSNLARLANLQSGTV
                     HLMMGTKQNFEMLTDLQNIEQDKENTRNEKMQLQDAIIEARQLSNMCSLNSQVFQELS
                     SYNNVLSNNLNDNVTTLTIISIGISIIAMVTSFYGMNVKLPFDSVDAVWVLIILITTI
                     ITIMLSIVMYI"
     gene            complement(42405..43364)
                     /locus_tag="VBC28_000263"
     CDS             complement(42405..43364)
                     /locus_tag="VBC28_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEO6814271.1"
                     /translation="MSKVRYGVVSTAKVAPRFIEGVRLAGNGEVVAVSSRTLESAQAF
                     ANKYHLPKAYDKLEDMLADESIDVIYVATINQDHYKVAKAALLAGKHVLVEKPFTLTY
                     DQANELFALAESCNLFLMEAQKSVFIPMTQVIKKLLASGEIGEVISISSTTAYPNIDH
                     VTWFRELELGGGTVHFMAPYALSYLQYLFDATITHASGTATFPKGQSDSQSKLLLQLS
                     NGVLVDIFLTTRLNLPHEMIIYGTEGRLIIPHFWKTTHAKLVRNDTSARTIQVDMVSD
                     FEKEAYHVSQMILEGQRVSHIMTPQLTLSGVKIIEDLYRSWGK"
     gene            complement(43535..46186)
                     /locus_tag="VBC28_000264"
     CDS             complement(43535..46186)
                     /locus_tag="VBC28_000264"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014623308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEO6814272.1"
                     /translation="MSKELSPKYNPAEVEEGRYQTWLDQDVFKPSGDTEAKPYSIVIP
                     PPNVTGKLHLGHAWDTTLQDIIIRQKRMQGFDTLWLPGMDHAGIATQAKVEERLREQG
                     ISRYDLGREKFLDKVWEWKDEYAATIKSQWGKMGLSVDYSRERFTLDEGLSKAVRKVF
                     VDLYNKGWIYRGEFIINWDPAARTALSDIEVIHKDVEGAFYHMNYMLEDGSRALEVAT
                     TRPETMFGDVAVAVNPEDARYKDLIGQNVILPIINKPIPIVADEHADPEFGTGVVKIT
                     PAHDPNDFAVGQRHNLPQVNVMNDDGTMNELADEFNGMDRFEARKAVVAKLESLGNLV
                     KIKKTTHSVGHSERTGVVVEPRLSTQWFVKMDQLAKNAIANQDTEDKVEFYPPRFNDT
                     FMSWMENVHDWVISRQLWWGHQIPAWYNVNGEMYVGEDAPEGDGWTQDEDVLDTWFSS
                     ALWPFSTMGWPDTEAADFKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGRQPFSNVL
                     IHGLIRDEEGRKMSKSLGNGIDPMDVIEKYGADALRWFLSNGSAPGQDVRFSYEKMDA
                     SWNFINKIWNISRYILMNNEGLTLDQARENVEKVVNSQVGNVTDRWILHNLNETVGKV
                     TENFDKFEFGVAGHILYNFIWEEFANWYVELTKEVLYSDNEDEKVITRSVLLYTLDQI
                     LRLLHPIMPFVTEEIFGQYAEGSIVLASYPQVNATFENQTAHKGVESLKDLIRSVRNS
                     RAEVNVAPSKPITILVKTSDSELESFFKDNSNYIKRFTNPETLEISSAIATPELAMSS
                     VITGAEIFLPLADLLNVEEELARLEKELAKWQKELDMVGKKLSNERFVANAKPEVVQK
                     EKDKQTDYQTKYDATIARIEEMKKLVR"
     gene            complement(46186..46752)
                     /locus_tag="VBC28_000265"
     CDS             complement(46186..46752)
                     /locus_tag="VBC28_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEO6814273.1"
                     /translation="MNIIIIGAQASGKMTIGQEIAKQTGMTLFHNHDSIDFVLRFMPW
                     SPDSIALTESIRFKFFETFAKTGQEMIFTIVIDFNDSRDVVFLEKIQIVFQSHNQEVL
                     FVELETELSERLKRNRTENRLKHKPSKRDIKWSESDICSTMDYAIFNPEVAPEALTYY
                     HKINNTCLTATETAYLIIQKINQIKEKN"
     gene            complement(46749..47333)
                     /locus_tag="VBC28_000266"
     CDS             complement(46749..47333)
                     /locus_tag="VBC28_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO6814274.1"
                     /translation="MIKILGEIVDNQLPVVETNRLLLRQRKLEDAKEIFEFVKLDEVS
                     YPAGFPAVKSLEEEITYIQEIYPTNLEKEKLPSGYAITLKGDDKVIGSVDFNHRHEDD
                     IFEIGYLLHPDYWGQGIVPEAASALVEIGFTLLGLHKIELGCYDYNKQSQAVARKLGF
                     TLEANIRDRRDAQGKRCGDMRFGLLRSEWEKKRR"
     gene            complement(47431..48000)
                     /locus_tag="VBC28_000267"
     CDS             complement(47431..48000)
                     /locus_tag="VBC28_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001166188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO6814275.1"
                     /translation="MQILEDFDGKALPKLETDRLILRQRTVGDVPAMFDYVCLEEVAY
                     PAGLSPIASLEDEYDYFENRYYQNLEKAKLPSGYGITVKGSDRIIGSCAFNHRHEDDV
                     FEICYLLHPDYWGHGYMTEAVAALIEVGFTLLNLHKIEIRCYDYNKQSRRVAEKLGFT
                     LEATIRDRKDNQDNRCVNLIYGLLRSEWE"
     gene            complement(48268..48579)
                     /locus_tag="VBC28_000268"
     CDS             complement(48268..48579)
                     /locus_tag="VBC28_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001119099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6814276.1"
                     /translation="MAKRIKPNKKRELLKAKGINWKDVRLSQTLQEVALPKKTAPEAP
                     KAVDLLEGLTANKQDVLKEAGLVSLEAFAKVSEADVLALKGIGPAAIKQLVDNGVVFA
                     K"
     gene            complement(48688..48942)
                     /locus_tag="VBC28_000269"
     CDS             complement(48688..48942)
                     /locus_tag="VBC28_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1912 family protein"
                     /protein_id="HEO6814277.1"
                     /translation="MSYEQEFLKDFEEWLQSQISINQMAMDSAKKVLEEDKDERAADA
                     YIRYESKLDAYRFLQGKFNNYHNQKSFHDLPDGLFGQRHY"
     gene            49253..49910
                     /locus_tag="VBC28_000270"
                     /pseudo
     CDS             49253..49910
                     /locus_tag="VBC28_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000018433.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
     gene            49905..50025
                     /locus_tag="VBC28_000271"
                     /pseudo
     CDS             49905..50025
                     /locus_tag="VBC28_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000484319.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
BASE COUNT        15702 a        10076 c         8067 g        16180 t
ORIGIN      
        1 atatttagat ataaaaaaaa gaccttcaag gtcttttata attatcagta taataaatcg
       61 taaatttcca ttgcgataag gtcaatgctg tcaaatgaat atgattcacg cgcttctaca
      121 tcacgtaatg tgaaaacagg accgttttca gggtcattac taaaagatac ttctgctaca
      181 acaacacctt cacgttcaaa attacgtttt aaattaccac catcagtcgc cattaattcg
      241 aggcgattga tgattctcac taaatgtgat tccatcttac ttactcctat atattttcta
      301 gtcttttcta ttttaacaca aaagtgacca aagcaacatg aaaagcaaca tttttactaa
      361 ctttttaacg attttatcgt ctaaaatccg caaataacga tttttctcag tagtcatcta
      421 tttttcaatt tagcactcta atatattcag tgctaaaaac agaacttttt tcttgatttc
      481 taggctttag gtgctataat aaaagcataa agtttgacta tatttgacta tatacacatc
      541 aatagttaaa tgtcataaga aaggggtagc ctatgctctg tcaaaactgc aaactaaacg
      601 aatcaactat tcatttgtat acaaatgtta atggaaaaca aaagcaagtt gatctttgtc
      661 aaaattgcta ccaaattatt aaaactgacc ctaataatcc acttttttca ggtttaaatc
      721 atgtttcaca tgcgcctggt ggcatcaatc ctttctttga tgatttcttt ggagatttaa
      781 ataatttcag agcctttaat ggccaagatt taccaaacac tcctcctaca caatcaggag
      841 gaaaccgagg tggaggaaac ggtaatggac gaaataataa tcgcaaccaa actgcaactc
      901 cttctcaagc caaagggatt ctagaagaat ttggtatcaa tgtcactgaa atagcccgcc
      961 atggtgatat tgaccctgtt attggacgtg actcagaaat tattcgtgtt atcgaaatcc
     1021 ttaatcgtcg tacaaaaaat aatcctgtac ttattggcga acccggcgtt gggaaaactg
     1081 ccgttgttga aggacttgct cagaaaattg ttgatggtaa tgtgcctcat aaacttcaag
     1141 gcaaacaagt tattcgtttg gatgttgtga gtctagtaca aggaacgggc attcgtggtc
     1201 aatttgaaga acgcatgcaa aagttgatgg aagaaattcg tcaacgtcaa gatgttattc
     1261 tattcataga tgaaattcac gaaatagttg gagctggtac agctggcgaa ggtagtatgg
     1321 atgcaggtaa tattttaaaa cctgcccttg cacgtggaga attacaacta gtaggtgcta
     1381 ctactctcaa tgaatatcgt attatcgaaa aggacgctgc cttagaacgc cgtatgcaac
     1441 ctgtaaaagt cgatgagcct tctgttgaag aaaccattac tattttgaaa ggtatccaaa
     1501 aaaaatacga agattatcat catgtaaaat ataataatga tgccatagaa gcagctgcag
     1561 tactatctaa tcgttatatc caggaccgct ttttacctga taaagcaata gacttattag
     1621 atgaagctgg ttctaaaatg aacctaacac taaattttgt tgatccaaaa gaaattgatc
     1681 aacgtctcat tgaagcagaa aatttaaaag cgcaagcgac tcgtgaagaa gattacgaac
     1741 gtgcagctta cttccgtgac cagattgcaa aatataaaga aatgcaacaa caaaaggtcg
     1801 acgatcaaga tacacctatt attaccgaaa aaacaattga gcacatcatt gaagaaaaaa
     1861 cgaatatccc tgttggtgat ttaaaagaaa aagaacaatc tcaattaatt aatctcgcag
     1921 atgacttgaa acaacatgtg atcggccagg atgacgctgt cgttaagatt gcaaaagcta
     1981 ttcgtcgtaa tcgagttggt cttggtagcc caaaccgtcc tattggttcc tttttatttg
     2041 taggaccaac cggcgttggt aaaactgaac tttctaaaca actagcaatt gagctctttg
     2101 gttcagctga tagtatgatt cgttttgata tgtcagagta catggaaaag catgctgttg
     2161 ctaaattagt cggagcgcct ccaggatacg tgggatacga ggaagctgga caattaactg
     2221 aaaaggttcg tcgaaatcct tactcgctca tccttctaga tgaaattgaa aaagctcatc
     2281 ctgatgtcat gcatatgttc ttgcaggtcc ttgatgacgg tcgattaaca gatggacaag
     2341 gaagaactgt tagttttaaa gataccatta tcatcatgac ctcaaatgct ggttctggta
     2401 aaactgaagc aagcgttggc tttggtgcct cacgagaagg tagaacgaat tccgtactag
     2461 gtcaactagg taacttcttc agccctgaat ttatgaaccg ctttgacggt attattgaat
     2521 tcaaggcttt agataaagag aatctcctca atatcgttga tattatgtta tctgacgtta
     2581 atgcacgtct cgccatcaat ggtattcatc tagatgtcac tgataaagtg aaagaaaaat
     2641 tggttgattt aggttacgat cccaaaatgg gagcacgtcc attacgtcgt accattcaag
     2701 aacatattga agatgctatc acagattact atcttgagaa tccaagcgaa aaagaacttc
     2761 gtgctattat gactagcaat ggaaatatca taataaaatc ttctaaaaaa actgaagaaa
     2821 gtacaaaagg ttgagtaaga aactcaacct tttttcacac tcttctcaaa aaataatagc
     2881 ctctataaag ctgtttctct cctacctcgt ctttaaattt taatacaatt attaaggaaa
     2941 tattccaaaa attttagtat aataaataaa aaataattaa agcttttaaa atcataaaac
     3001 acaatcagga gagagaacta tgactaatcc tactttcggt gaaaaaatag ataatgtaaa
     3061 ctataggtca cgatttggtg tctatgctat tattccaaat ccaactcatg ataaaattat
     3121 tttagtacag gcacctaatg gtgcttggtt tcttccaggt ggggaaattg aagaaaatga
     3181 aaatcaccta gaagctttaa ctcgtgagtt aattgaagaa ttaggttatt ccgcaacaat
     3241 tggccattac tatggtcaag ctgatgaata tttttactct agacaccgtg atacttacta
     3301 ctataatccc gcctatattt atgaagttac tgcctatcat aaggatcaag cgccactaga
     3361 agatttcaac catctagcct ggtttcctat ccaagaagct aaagaaaagc taaaacgagg
     3421 aagccataga tggggtgtcc aagcttggga aaaaaatcac cattctagga aataattcat
     3481 acctttcttc ctaaatagtg ttataataaa cctaataaga atcaggggga aaatagttat
     3541 gagactaatt aatacaacca gtagtcatcc tgaactagtt cgaaatcaac tccaaaatac
     3601 agatgcaaaa ttagtggaag tctattcagc tggcaacacc gatgtcgttt ttacaaaagc
     3661 acctaaacat tatgaattat taatttcaaa caaatatcgt gctatcaaag atgaagaatt
     3721 agaagctatt cgtgagttct tcttaaagcg taaaatagat caatctatta ttattcaaga
     3781 gcagatgaaa tcactccata ctgccaagtt aattgaaatt tcatatccaa caacagcata
     3841 attaaaagaa gcctaatcaa ggcttctttt ttgatgacta ctaaacaaat gtcttaccaa
     3901 tttcttccat taaaactggt aagatatttg tcaaaatata gcgttaaata atttttatta
     3961 agcttcaaaa ccttgagcta ctgcttcagg ataatattgc tcaataattg ctgcactagc
     4021 atcacaaaca gctacaccat acgaacgcat ctttgtggtt ggatcaatac ggcgtgagcg
     4081 ttcgcagact tcaccttcag cgtgttcaac agtaaatgca acaccttcaa atgatactga
     4141 gtcagctggt ttgtctgctt catcagcaat tgttaattgt gagacaatca tcaagagtgc
     4201 aatatcgcta tttaacgctg ttaataacgt tttcacttct tgactagcat aaattgttaa
     4261 atgtgcttct agagatttac caataacttt agcgttacgt gcttcttcga gagctttttg
     4321 agcttgtgtt cgtaacgtca tgaatgctga ccactcttca agaatttctt cttgaccaga
     4381 gaatgtctgt gcaacaggca tttctgctaa ttgaacaaac tcctcctcct catgttcgag
     4441 gtatgaccaa atttcttcag cagtatgtgg tagaatcgga gtcaaaagtt ttgtaagttt
     4501 aactaaaata tcatagaaaa cagtttgcat acgacgtcgt tctggactgt tagccgcttc
     4561 aatgtaaacc acatcttttg caaaatcaag gtaaaatgct gatagatcaa ctgttacgaa
     4621 gttaacgaca gctttataaa ttgccataaa atcataagca gcataagctt tgttgattgt
     4681 atccacaact tgattaaatt taatagtcat ataacgatca acggcaccta aattttcata
     4741 ggctactgca tcttgtttag gattaaaatc ggaagtattt gcaatcaaga aacgcaatgt
     4801 attcctgatc ttacgataag tttctgatgt ttgttttaag atatccattg aaatacgaac
     4861 atcattactt gagtcaacag aagttaccca cagacgcaag atttccgcac caaattgttt
     4921 ttcaacatca ctaggaagaa ttgtatttcc tagagattta gacattttct cacctttacc
     4981 atcaagtaca aaaccttgag ataagactgc tttgtatgga gcgtgaccat taacagcaac
     5041 tgatgtaatt agtgatgagt tgaaccaacc acggtattga tcagaacctt caagataaag
     5101 atctgctgga taggaaaggt tttcacgagc attcattacc ccattccatg atgaaccaga
     5161 atcaaaccaa acatccatga tgtctgtttc tttctcaaag agaccattag gtgaacctgg
     5221 atgagtataa cctgctggta gaaggtcctt agcatcacgt tgccaccaaa cgattgagcc
     5281 gtattcagca aataaatctg caacgtgatc agtcacttct tttgtcatga tagctgtacc
     5341 atcttctgca taaaaaattg gaagtggaac accccaagca cgttgacgtg agattaccca
     5401 gtcgccacgg tcacggatca tgttataaag acgtttttta ccccactcag gttggaaatt
     5461 tgtcttttca atttcatcga ggatttcttg acggaatttc gaaacagagg caaaccattg
     5521 tggaaccgca cgccaaatga ttggcttttt cgtacgccag tcaaatggat atgagtggtt
     5581 gataacttcg ctggctaaaa gaagatctcc caatttttct tttacaagtg gtgttacttt
     5641 gtcatagaat tgaccttcaa aatcaggacc agcattttcc atcatcaaac cacgactatc
     5701 tacggtaact acaacatcaa gtccattagc aataccaacg ttatagtcat cttcaccaaa
     5761 accaggagcc gtgtggacaa tacctgtacc agaatctgtt gtaacatggt ctccaaggat
     5821 aaccaactct tctacttctg tatcccatgg atgttctgta acaatgtgat taagttcttt
     5881 accagtgtga tgagtcacaa tttcaaagtt ttcccagcca aacttagcag cgagactatc
     5941 tacaagaacc tctgcaagaa ggtatttacg ctcactacct actggtacaa ctacaacata
     6001 ctccatatct ggtcctactg ttaaaccgcg tgaagctgtt acagtaaatg gtgttgtcgt
     6061 ccaaacgacg atataggtat ctgtatcaag aattccctta ccatctttaa ctttattggc
     6121 atagtagagt gatgtcgaat caatatcatg atattcgatt tcagcctcag caagggcaga
     6181 ctctgatgac catgaccaat acactggttt agcaccacga tagatatatc ctttatctgc
     6241 catagcaccg aaaacacgta cttgatctgc ttcataatct ggtgttagtg taatataagg
     6301 attttcccaa tcggcagaaa cgcccagacg tttaaaatca tcacgttgtt tgtcaacttg
     6361 gctgagagca taatcacgac acatttcaag gtattcagcc aagtccattt ctttacgttt
     6421 aacccctttt ttagctaata cttgctcaat tgggagtcca tgagtatccc aaccaggcac
     6481 ataaggagct cgaaaacctg acattgattt tgaacgtaca ataatgtctt tcgaaatttt
     6541 atttagtgca tgtcctacat gaatattccc attagcatag ggaggtccgt catgaaggtg
     6601 aaaggctggt tttccttcat tcaatgcttg acgtttttta taaatgtcag cttgatccca
     6661 tgcttcttgc cattgaggtt ccttatttgg aagccctgca cgcattggaa aagctgtttg
     6721 tcctaaattc aaggtttctt ttaatttcat actaactcct tatcttatag taatagttat
     6781 cacaattttt taaattgcta aataaaaaag ctttcatcac ataaaggacg aaagctcgtg
     6841 gtaccacctt aattcagagc agaaaatagc tatttcttac tccctcaaca acccgtaagg
     6901 tgggttaagc cttctacctt acttaaataa tagttcaggt agaatgattg aaaatgataa
     6961 tttcaatatt agggattgta gggctctcac catctcctac tcgctgataa tataatatta
     7021 aaaccttgtt tttcttaatc ttcaatattt aatttaaaag tttgagtttc acctaaattg
     7081 atcggttctt ccatttggaa attattagta tctaataaac ctgactcttc aagttgtttg
     7141 ttactttctt caacacgacg ctgtaattct tccatttcat ctggtgagaa ttgacgagta
     7201 gcatcaaatg actcagtgtc atccaccact ggtaaagcat catcttcatc aagaactttt
     7261 tcaacaactt ctttgaaaga agcatcagaa ttttgaagat aaatagctgt tggtttcaac
     7321 aattcttccc aagcagatga gttagctaat tttagctgcc cttctaactc agaaagcaaa
     7381 cgttggtgga aaacacgtga ttgacgtttc aaatcttctg tttctatagc aacacgctta
     7441 gcatcatctg tcgcatctcg aaggatttga tttgctttta atttagcctc atcaattaaa
     7501 tgttgagcat caaatgtagc tttccccatt aggttagatg cttcatcctg tgctgaaatt
     7561 ttcacacgct cagcagtttc ttgagctaaa ataactgatt gacttaacga ttccttcatt
     7621 tcgttgaagt aagcgatttt ctcttctaaa tctttaatgt attgctcttg ctcacgatta
     7681 cgtctaatca agtcctcgta atcgtcaaca acaatctcta aaaattcatt aacttcttct
     7741 tcgctataac cgcgaaattt tgatgaaaat gttttatctt taatttcaag tgctgtaagt
     7801 ggcatgtttt ctccttagtt atgtatcatt ttgtctactt ctaacttata tttttgattt
     7861 ttagttaacc ctaggttatg gttcaacgta aatcgaccat atcctctaac actaatcaaa
     7921 tccccctcta ctaattgttc agaaactcga ttgacggttg cataattgac cttgacttta
     7981 tctgcctcaa tcaatttcgt cgactgtgtt cgtgaaatct ttaaaatcgt tgctaaaatt
     8041 ttatcaagac gtaaactgga agcaataaca gttagttttt gaatatcttg tttgggagtt
     8101 agaagtttgg acaagggaac ttcagctaat tgtacactag cagttccaat ttttgtaact
     8161 gaatgaacaa ggtgatttgt catttgacta tcgaccaaaa cctgagcaca gccttcttca
     8221 actaggatat cccctaaaat tgatcgtttt acacctaagt aattcagtaa agttcccata
     8281 attttagcat gggtaaggtg attaaattta gcattatact ttatttcaat taggctaagg
     8341 ttaaaatcag ccatatctaa ctgatagtat tccggtgcta ttattacttt aacatattct
     8401 gtttgaaaat actgaccaga agtgtaataa tgactacctc gcctctctag aacactttcg
     8461 agtattttaa attccctagg atttagaaat tcagtggtaa taaacgaata ggtgttttcg
     8521 acatattgag ctaaatggtc tattttatgg ataaacgcat actcttctgg tcgaaaatgc
     8581 tgataaatat catctaacgt catcttatgt aaaaagcaag attaaaaatc tactcaacat
     8641 atttagtaaa aagaatgcta ctattactgt aaaatctaga cctaaaaatt gtagattaaa
     8701 tcttctgagt gggcgaataa tcggatcaac aatctgtacc actagtctac caatccatga
     8761 atcgtatgct cctggaaacc aagataacaa ggcataggct agtaagatat aagaatagac
     8821 cctaattgtt tttaataata ttaatagtac taatatcata tttatcgtct cttcatatca
     8881 aaatcaaaac tcgtctcttg accagtcttt ggaatattca tctcctcaat atcaaccata
     8941 acattggctg gtgttaatag aaacattgaa ctcccaacct tttgtaatga accatataat
     9001 actctacttg caccatcaat ataatccaag caacgtcgtg cttgcgcatc aagcatatat
     9061 tgaaaatcaa tcaagacaca ttcattaacg ataagtaaat caacaatttc ttgtgcatcc
     9121 tcatacttac ggggatactt cagagcaatt gttacttgtt ctctctgttg tcgaactgag
     9181 tctttatttt gaaccctagg agtagcttgc tgataagcat tgtctctctg taccctttgt
     9241 ctctcctgac gattaggatc ataggtataa gttctcggac gactaccaat catctcttcc
     9301 tctgcggagt tagtcatatg actacgctga gaagcttcct gtgctgtagc tgctctggaa
     9361 tctctttgca ctgaagttct ctcttgtact tcgtgtactt cattttcact tacatcgtca
     9421 gtgtcaaaat aagaaataat tttgtcaaat ctatctttta atgccatatt cccctccatt
     9481 atttaaagaa tgaagttcct atccttacaa atgttgatcc attttgaata gcaatatcat
     9541 aatctctact catgcccatg cttaattctg taaacggcat tcgttctatg ttcctagctt
     9601 gaagcctttt cctaagactc tcagtctctt caaaaattga tgatatttcc tctttagttg
     9661 cttttaaggg agccatagtc atgatgccga taagttcaat tttatcgtac cgagaaatta
     9721 agttcagtgc atcatctatt tgctcaatag taaaaccatg cttactgtct tctcgtgata
     9781 tattaacttg tagaaaacat ttaattggtt tttgagcacg tttttgaatc tcagctgcta
     9841 acttaacaga atctaaagca tgaaaataat caacataatt aatgacatct ttaacttttc
     9901 gacgttgtaa actaccaatg agatgccatg taagcttttc atcttttaat gcttgatatt
     9961 tttcaagaaa tttatcaaca cgattttcac cgatatgatt aacacctgtt ctaataagcg
    10021 cttctgttgt ttgacagtta acatattttg ttacagctac gatatgaact gattcatgag
    10081 cgcgacctgc ttttagtgct aatttactaa cattgtcaaa aatcgctgtt ttattttctt
    10141 gtaaattcat cattttaatt aacggttttt aaagaatgga ggtgtttcaa gttcatcatc
    10201 catatcatca ttttctgaaa acgttgacat agacaacttg ctatcaagtt caccttctgt
    10261 cggacgtgaa atattatcac gacgaaggtc ccaattacca aaagcagaac tttgttgctg
    10321 attttgtgca tgaggctgat tttgttgagt tggcatttcg cgagactctg tcatatcaaa
    10381 gttgccacga cgatcaaagt ttgaatttga agtactttga cgttctgaag gtgcttgatt
    10441 agttggagct gatgttgtaa aaccagatac ttgattagtt ttatctttac gtacacctgt
    10501 tgcaacaact gttacgcgga tttcgtcttt catatccatg tcaattgaag tacctaacca
    10561 aatattgaca cctttacctg ctgcttggct aacaatctct gatgcttcct ctgcttcagt
    10621 aagtgtcata tccataccgc cggtaacatt gacaataacg tcttctgctc catcaatcgt
    10681 tgtttcaaga agtggtgaat aaattgcttt tcgagcagct tcagtaatac gttcctcacc
    10741 agaaccgata ccaattccca tcagtgcatt acctttattt gccataactg ttttaacatc
    10801 tgcaaagtca aggttaatta aacctgggtt agtaatcaaa tccgtaatac cttgaacacc
    10861 ttgacgtaaa acattatctg cttcactaag agcttcaagc aatggtgtct tcttatctac
    10921 aatttcaaga agattattat ttgaaatgat aagcagtgta tcaacttgtt ctcttaattc
    10981 ttgaatacct tcgatagcga agttagaacg tttattaccc tcaaagccaa aaggacgtgt
    11041 gatgaccgct actgtaagtg ctcctaaact ctttgcaata cgtgcaataa ctggagccgc
    11101 acctgtacca gatccgcccc ccatacctgc tgtgataaat accatatcag cgccagtaag
    11161 tgcttctgtt aaaacttctt cactttcttc agctgcttta cgaccaactt caggttgtcc
    11221 tcctgcacca agtccgcgag ttaattttgg accaagttga atgacagttt cagcttttga
    11281 gctacttaag gcttgaatat cagtatttgc tgcaataaac tcaacgccag cgacaccttc
    11341 gtcaatcatg cggttaatag cgttaccgcc accgcctcca acaccaataa ctttaataac
    11401 tgcaccttgt actgatgctg tatcaaatga aaataccatt tttattcctc acttattgtt
    11461 ttagtcaaac atacttccaa aaattcctct gatgcgctca ccaatattag ctttaggctc
    11521 agtaggctct tgagcgtttt ctcttgctcg gattggttct aaattagaat cttccataga
    11581 agaggttaac ggctcagaat aaggcattgt cgacattgta tttgtttttt ctttataatc
    11641 aaagtcgact ggtttatgtc ttagcatttc gtcgccagtt acagcatgtt gtgcgataat
    11701 gtcaacttct gacatcattc ctacataatc aacgatacta ataacattag caaacattgg
    11761 attgcgaata cctacttggt ttggaacgtg caatttaact ctagttccaa aaatttgttg
    11821 agcaacctcg acaactcctg gcataattgc accacctcca acaagaataa taccaccagg
    11881 taaatcaagt aaacgacctc tacccaagtc ttgtttgaca tgttctaaaa tttgacgaat
    11941 acgtcctgaa ataatttgcg aaagatagct ttctgtaatt tcaacaggtt cctcgttacc
    12001 aacaacatta acttgaactg tatcagaagt acttgcttct tcaacattag cttgtccaaa
    12061 gttgaatttc aatgcttcgg cgatttctac tgttgtacgt agcactttag aaatatcttt
    12121 ggtaacataa tcgctacctt cagaataaat attagtatat tgcaattctt gattacgcat
    12181 agaagcgact gttgtttgtc cgcctcccat atcaatcaca gttgcaccaa actcacgttc
    12241 accttcgttt agaactgatt ttgctaaggc tagaggggcg atcacaacat gctctacttt
    12301 tatacctgct cgttcaacag ttttacgaag attatgaagg atagttgttg gtccagtata
    12361 gataagacca cgcatctcaa ggcgaatccc catcatccct ctagggtctc taatcccttg
    12421 gaaaccatct acaatgaatt caagcggaat caatgaaata acttctcttt caggagtaat
    12481 gcttttagtc aatgctgatt taacaacact ttcgacatct tcatccttaa tttcttttga
    12541 ttcattagga acaggaatca ttccttgagt tggttcaatt tggagaagat ttgctggtaa
    12601 tccaacattg attttgtcaa tggtgatgcc tgctttctcc tcagcttgtt ttaccgcctc
    12661 tttgattgca gttgctgctg cctcaatatc aataattata ccatctttta caccggaact
    12721 agggacgttg ctaaccccaa ttacattcat ttcatttgca ataaattctg caactaaaac
    12781 tttaatcgag cttgttccta tatccaaacc agtaaaaaag ccatttctag ccattcactc
    12841 gacctcgcta tctttccatt cttttctatt ctacatctgc caaaaacaga attattcaga
    12901 aaatattata ccataaaaaa agcagaattg ttaccaaatt acgttttttt attaagcaac
    12961 atcaaattga aatatttcta acttatttaa accattcgga acttataaca aacttaaatt
    13021 tttttcaaga aatcaatttt tactagcaat ctatgactga cttgatatat ataaaaaaga
    13081 ctgataaatc cagtcttttt tattgctaat taacattttg agggttaggt gcctgctctg
    13141 ttgctatctg ttgtccttgt tgattagtat ttgagttatt tgtttgtcct tgactatttt
    13201 ccgcaacctg accattttgt gtttgtgttt tatcagttga tttatttttt gtatcttctg
    13261 ctttaacagg ggttgattca atggtatttg ttgttgtgta aactcccact tccatatcaa
    13321 caatagaagg ttccttaagg ttcttcttaa tttgtttgta aaaaggaagt ctttctttaa
    13381 atttagataa tggtattcta atactatttc catcgtgcat atctaacagc aggaggtcag
    13441 gtgtcgtttt agaatcagct aaacttatca cctgaatctc acttattaaa tcagggtcta
    13501 aagcctttaa atctttaatt aatagcttaa tactatcttc cttatcaagg ttaattgtta
    13561 agaagtgctt tggtagctct gaactattta caggatcagc cttttttcca gtttccaaga
    13621 caggttgata tccttgcttt gtatgtgcat atgcaataat cttattttct tgaacttgaa
    13681 tatgaaactt attgggaaat tgataagtca tctgagctgt ttttacccat acatcttctg
    13741 cagctaaacg ttgttcaata gctttatgtt taaaaattaa agaaaagaaa taatcgtttt
    13801 tttgaatatt cgttttctct atcaaaatat catcaggtgt atgctgattt ccactaactg
    13861 ttattgtttt ttgcttacta aaaggagtta gtaggaaaac ggaaactaaa atgactagga
    13921 atgctactac aaatataggt gcagttctaa tgcgattagt tttggctaag ctgttgacta
    13981 ttttttcttt tttctgtttc ttttcaatct taggtcttga aatctttgga aaatgaagct
    14041 tcttaatttt agtagtattt tgaggttctt caggagaaga aatatttaat ttacttcttt
    14101 tatctaagcg taatttttcg ttaatacgtt tttgttcttc ttcatcttct ttgcgttttt
    14161 ttaaaaattc aaggttacgc ttttgccatt ccgttaagac aacttcttct ttttctgggg
    14221 tatctgattt cttcttaggc atgttttatc cttttgttac ttttgccata tcatcaatta
    14281 ataattgata aaattcatct tgagatttta tttcagatga ttgtgacatg tttttttcgt
    14341 aactctcact gttagaaatt aataaattta tctgtttctc aagtgtattt atgtttaatt
    14401 cagattctgg taactgcaat gcgtagccct tttcttcaaa ataagcagca ttctctaatt
    14461 ggtcacctcg gctcgcttca cgtccaagag ggataataag atgaagtttt ttcatagcta
    14521 ctaattcaaa aatagtatta gagccacctc gtgtaacaac cacatctgct aaattcatta
    14581 aaggctgata aaggtctgta acataatcca cgcgataaag gttcttcttc aatcgattga
    14641 gagaagaatc gcctgaaata ttgataacat tatatttttc ttccagctca ggtgtttgag
    14701 taataaaatc attgaatact tttgcacctg ctgatcctcc aataaatagt aaggtcttta
    14761 ggtttggatc gaaggcttct tttatactag ttaagtcagt attttcaaac gactttttac
    14821 aatccatgac ttttgttaca gcaccaatat gcttagtttt gattaaatct ttagattgtt
    14881 caaaagtcgt atacataata gtcgcaaatt tataagcaat cttatttgct aaccccattg
    14941 ataaatctga ttcatgaaca aaaacaggaa cttttaatag gcgtgcagct actacaggag
    15001 gtactgaaac aaatccccct tttgaaaata aggcttgggg tctaagttta gcaataatag
    15061 caatcgattg aagaacgcca acccctactt taaagacatc taacatattt tgccatgaaa
    15121 aatagcgacg tagtttccca gtagctatag agtgaaatgt gatatcaagt ccagactgat
    15181 taatttgttc atgttctatg ccatttttat caccaatata atgtacttcc caaccatctt
    15241 tgataaattt ggggattaat attaggttta gtgttacatg acctaccgta ccaccacctg
    15301 taaaaacaat ttttttcccc atagttactc tcctctaaga ctttcgaaag tatcaatgaa
    15361 ttcatcacca cggacttcga aattcttata catgtcccat gatgcatttg caggacttag
    15421 caagataaca tcgccctgtt gtgccacctc ataagcttta tgtaccgcat ctctaacatc
    15481 taaagcatcg ctataagtta ctcctgcttt ttgtgcagca cgttttactc gagatgccga
    15541 ttcccctaaa acaaccatat gtttaagtcc agtgatatct ggtatcaatt catcaaactc
    15601 attaccgcga tcaagacctc ctgcaattag gataacttta gtattatcaa agccagataa
    15661 tgctttttga gttgccaata tattagttga cttgctgtcg ttatagaaac taataccatg
    15721 aaccttaccg agtgattgca agcggtgttt aacacctcca aaattgctta aagtttctct
    15781 aataacttga ttactgatac cagccagttt agcaaccgca atagttgcta gagcattctc
    15841 tacgttatgg cttcctggga caccaatgtc atctactgac ataatattct cccctttata
    15901 gaaaagttgc ttgtcttgta cgtaagcacc atcaactttt tccgtagtag agaaaggaac
    15961 gattgttgct ttagtagttt tagctaactc tttagaaata ccttgattaa aattaagtac
    16021 caaaaaatca gatgaagaca tttgattttg gatattccat tttgcagcaa cataatcttc
    16081 aaaagaccca tgataatcta aatgagttgg cattaaatta gtaattactg caatatgagg
    16141 acgaaattcc ttaactccca ttagctgaaa acttgataat tccataacta gagtatcttt
    16201 atcattcgca gcctgaacaa cttcactagc aggaaagccg atattcccag ctaacaaacc
    16261 tctctgacct ccagcattta agacttctgc aatcatcgtt gtcgttgtcg ttttcccgtt
    16321 agagcctgta atacctatta gctgagattc tgaaactaag tatgctaatt ccacttcagt
    16381 caaaacaggg atttgttttt ctaatgcttt tttgaccata ggattgttat aaggtattcc
    16441 tggattttta atcatgtaac aaaaatcctc atctaacaat tctaaaggat gactaccaca
    16501 aaccacttta ataccctctt ccaacaaaga ctgtgctgtt ggattttcat caaatggttt
    16561 gccatcatta actgtcacta ttgctcctaa cttagctaac aaacgtgcag cagcttctcc
    16621 agatcgtgct aaaccaagga ctaaaacttt tttattttca aatgttgtta ttgttttcat
    16681 tactcgtccc attctatcat tattatccct attattttac ctttaatcag tggctttttc
    16741 aaggtaaagg atatgtctct aatttctcaa aacaaaagaa cttaggagac aaaatcctaa
    16801 gttcataagc tatttcttct tttctgacaa attcaatgtt tctttaaatg ctgtatatcc
    16861 tagaggaatc attataagcc caatatacaa ttcacctggc aattgaagga ttttaataaa
    16921 actcataact gccaagacaa gtaataaggc taccaaacct actaaaccaa tagcgttcaa
    16981 agtattacgt actgtttttg gtgccataaa aaagtaatac aaggcaatca aaagggcaac
    17041 aattaagtaa aacatagaac tcctttctta ttaatcataa ataagaaata ctttggatat
    17101 tattcagctg attttttctt tttattagct ttgtcacgtg ctgctttatt taaaatttgt
    17161 ttacgaagac gaatagactc aggagttact tccatgtact catcatctgc taaaaattca
    17221 agtgactctt ctaaagtaag gatacgaggc gtcttgatga ctgcagtttg atctttagtt
    17281 gctgaacgga catttgtcat ttgtttagca gttgtaatat tgactccgag gtcattatca
    17341 cgagaattct caccaacaat cattccttca taaacttcta tacctggatt tacaaagata
    17401 gtcccacgtt cttcaatacg cataattgaa tatgtagttg ctttaccatt ttcaatagaa
    17461 accaaggcac cacgatgacg accaccaatt tctccttgaa caaccggtag atactggtca
    17521 aaagtatgat tcatgatacc atatccacgt gtcattgaaa gaaactctgt tgaataacca
    17581 atcaaaccac gtgcaggaat caagaaaatc aaacgcgttt gaccattacc aaccatctgc
    17641 atatcaagca tatcaccttt acgctctgac aaactttgga taatagcacc ctgatattct
    17701 tctggagtat caatttgaac acgctcaaac ggctcgcatt gaacaccatc aatttctttg
    17761 atgataactt ctggacgtga tacttgaagt tcatatccct cacgacgcat tgtttcaata
    17821 aggatagata aatgtaattc tccacgccct gaaaccgtcc atttatctgg cgaatctgtt
    17881 gggtcaacac gtaaagaaac gtctgtttgt aattctgcta aaagacgttc ttcaacctta
    17941 cgtgacgtaa tccatttacc ttcgcgacct gcaaatggtg aattattcac caagaaagtc
    18001 atttgaagtg ttggctcgtc aatacgtaaa actggtagtg gttcaatagc atctgtcgga
    18061 gttactgtct caccaacgaa gatatcttcc ataccagaaa cagcgattaa atcacccgct
    18121 ttagcctctt ggatttcttt acgttcaagt ccaaagaaac caaaaagttt tgttacgcgg
    18181 aagtttttag ttgtaccatc aagttttgaa agagtaactt gatctccaac tttgacagtc
    18241 ccgcggaaaa cacgtccaat accaatacgt cctacaaaat cattgtaatc aagaagagaa
    18301 acttggaatt gaagtggttc ttccgagttg tcaactgggg ctggaatgtg atcaatgata
    18361 gtatcaaaaa tcggtgccat tgttttttct tgatctgaag gatcatctga cattgaagat
    18421 gttccattga tagctgaagc ataaacaaca gggaaatcta gttgatcatc atcagcaccg
    18481 agctcaataa atagttcaag aacttcatca acaacctctg atggacgagc tgacggctta
    18541 tcaattttat ttacaacaac gattggaatt aagttttgtt caagagcttt cttcaaaaca
    18601 aaacgtgttt gtggcattgt tccttcgtag gcatcaacga ctaaaacaac accatcaacc
    18661 attttcataa tacgctcaac ttcaccacca aagtccgcgt gaccaggtgt gtccataata
    18721 ttgatacgaa catcgttgta tgctacggct gtattttttg caagaatggt gataccacgt
    18781 tctttttcga tatcatttga atccattgca cgttcttcaa gctctttacg ctcatcaaga
    18841 gtatgtgatt gttttaataa ttcatcaacg agagttgttt taccgtggtc aacgtgggca
    18901 atgatcgcaa cgttacggat atctgttctt aaatttgtca tgttttcctc tattttataa
    18961 tgtaattctg ttaacttatg aattataaca caatttttct taaaaaacag aatttacaca
    19021 agtgtaaatg ttttcatatt aactgcctat ttgtgataag aaaacttttc atcatttcat
    19081 tatttatgcc atttattttc tacttttttc aatttaattc atttctacaa aaagtgaaga
    19141 gagttattaa tagtaactct ctccttagta tattacttaa ctctacccgt ccagtaattg
    19201 aaaccgtcct ttaacacgta aagttgatta aaaccttctt ttctgagtaa caaaacaatc
    19261 cttggaatag attgaccacg gcttgcgtca tataagagga caggtttatc tttgcgcaaa
    19321 gccgataagg caactttaaa ttgacttgct ggaatgttac gtgctccgag aatatgcttt
    19381 ctatgaaaag caccagcttc tcggatatca attaactgtc ctcgtgacat ttctttttga
    19441 aaagactcgt tatctaaaaa tttagctgcc ctacgaaccc tccaataatt ccaagatgcc
    19501 caagcaacaa aagccagaag aataacaata attaaaataa cagacatatc cataccttat
    19561 ctccttaact tgcttgttga ttggctaagc ttgctgcacc aataatacca gcatcattac
    19621 ctagttcagc aatcttaatt ttagttgact ttttaacttg tgggaaagca aatgtgacaa
    19681 agtatttctc aacgcgacta cgtaaaaatt cacctgctgc tgagacaccg ccaccaataa
    19741 ccacagaatc agggtttaaa atatttgaaa tattagctgc tgccagtcca aggtaacgtg
    19801 atacacgttc aacaacagaa ttagcaaatt tatccccatc ttctgctgct ataaaaatat
    19861 ctttacttgt aacagtatca ccgttgtcaa tcgctgcttt aatggcagac gaaccctcat
    19921 attgttctgc gagttgacgt gctactctaa caacacctgt cgctgatgca actgtctcaa
    19981 ggcagccttt gttaccacat gtgcacgtaa atccattttc tggatcaaca atcatatgcc
    20041 caatttctcc acctgctcct gcaacaccat ggatgaggtt accatctgcg ataacacctc
    20101 cacctactcc tgttccgagg gttacgaaaa caacgtcggg attattggca ccagcaccta
    20161 cccagcgttc accaagtgct gcaacattag catcgttatc aataaaaaat ggaattccaa
    20221 cttctttttc aataactgaa cctacttctt gagtatcagc ccaatttaga ttaaaagcac
    20281 ctgttactgt tttactagtt ctatcaacag ctcctggaga acccataccg ataccgagaa
    20341 agtcatcttt tgttaatcca tagaggctca aacgatgttt gagagattca acgatatcag
    20401 aaacgatatg tcttccgttt tctaaagtat tggtctcaat tgcccatttt tcttgtactt
    20461 ctccctcaag cgtcaagata ccaaatttaa tggtcgttcc tccgaggtca attcccaata
    20521 atttcttact cattttttat tttccttctc caattctagt ctgtgctctc ttcttaaaat
    20581 cagttctgct tttaaataat catctctact tatcagacca ttatcgtaga gacgttgcaa
    20641 ttcaatcttc ataacttcga tatcataaag acgtttacca atataaacat aaataccaaa
    20701 ttttttcaaa agattctgca catcgaataa aactttcata tatgaatttt aacagaaaac
    20761 cattcaattt tcaagaaaat ccgcaaccgc ttgctaattg tcattatgac gttagactga
    20821 ctatctataa tagaaatcgt aatctctttt ctaccatttt tcgaatataa atataaacta
    20881 taaaaaggat tttatgatta tgtctaccat ctattttatt tctttatgca tgagttttat
    20941 cttaagttat tatgatataa aataccaaga gtatcctatt tttctatgga tattattcac
    21001 catttcaacg ataattctca caccgattac aaaagtttct attgttttgt gtttatttgg
    21061 gattttagct gaagtggttg atattaatat cggaagtgga gatttccttt atttagccac
    21121 aattggctta tctttacctc ttcatcaaat gctttttatt attcaaatag gtgcttggtt
    21181 aggaattatt tattgtttag ttatgagaaa aatgaaaaaa acgatagctt ttctaccgtt
    21241 tttaagtatc gcttatataa tcgtcaccag ttactctctt ttattctaat cactttaaat
    21301 tatcaggaaa gtgataaagt tgtgggtact ggtcgcattt tggattttta ggatgacaaa
    21361 ttgcacgacc aaaataaatc atggattgat gtgcagctag ccactcttct ggtggtaaaa
    21421 cctccatgac tcgtttctca atttccagtg gagaagccga ctgtttgcaa atctggtggt
    21481 gtttacaaat ccgagtgaca tgagtatcta cggcaaaagc cggaatacca aatccaacac
    21541 tcatgaccac gttagccgtt ttccttccaa cgccagccaa actttctagc tcttgacgag
    21601 ttcgggggac ttttccatca aaatgctcta tcaattgttt agcgcattga tttaagaatc
    21661 gagctttatt acggtaaagc ccaattttag aaatataggg ttcaatctct ttgggatcag
    21721 cttgtgccaa aactaaagga tttggaaagc gttcaaaaag ggcgggtgta actttattaa
    21781 cagctgcatc agttgtttga gctgatagca ttacagctac caaaagctca aagacattag
    21841 taaagtctaa gctaggttta gcatctggaa ataacttaat aatctcacgg ataatatatc
    21901 gtgattttgc ttttgataac attttattcc ttatctttct tttttatcat tatatcacga
    21961 tatctttcaa aataaagaat atcagaactg aggtttacaa tccattaacc ctattttatt
    22021 tttttagtaa aagctcatgc taactgtctt ttgagttgtt gataatcaat ttttccagat
    22081 ggcatcaaag gaattttaga tacaatatga taatgcttaa acccatactt cttttcaaaa
    22141 tgttccgata accacttcaa acactcttgt tgcatctgat tttcaattaa aacaatacag
    22201 caatgtgact cttgtccatg actttgactc tcttttgtaa atgctactgc atccttgata
    22261 taagggcatg tttttatttt ttcgattaat ctaggtaaat agagcttaat gcccgattga
    22321 ttgcaccaat caccaccacg gccaaataga tataatttgc catctatcag ttcaccataa
    22381 tcaccagcac aatattcact tgaaaggcca cagatactat acggtgtttt tacaaaaatt
    22441 cttccctctt taatcctaac agctacattt ggaacgattt ctccaacata ctgtggctta
    22501 tctcggatat cacgaccatc gtaccatgac acataattga gctcactagc accatagaag
    22561 attttcactg acactttagg atgctttcta tataaacctt ccagtaatga gtcagagaca
    22621 tattgcgaag aggtaataat agttttattg tctaacgcag tctccttgct atattgttcc
    22681 actaacttga gataactagg gagcagatat agatgcgtta caccagtttt ttcccataaa
    22741 gtctgccagt attttacaga atttttctgt gtgacaacaa gtgttccccc cagtaataaa
    22801 agggatagcg ctaaatttaa gttaccagta aatgaaaaat caccttgaat aaacagtttg
    22861 ctattggaag tgaccgaaaa ataagcattc tgaatgctaa aaaaatcaga ccacgaagtg
    22921 aagctcctcc acaatagttt agcatcagct gtagtcccag aagaaagaac accaaaatca
    22981 gctagttgag gagcatactg cacttctttt tctaatcgac ttatttcatt atgagtaagg
    23041 ttaggtttac aaataatagg aagagctttt tgacataact ttgaaacaaa tcttactaat
    23101 tggttaagca gagagtgtgt gctaatcaca tgtttcctat tgtcttgtga cgccatatct
    23161 tgtcttacta agttataaaa ttctccataa ctgacctgta aatcaccatc aaataacttt
    23221 ttatcgctat tggttttaac tatcgttttc aatgattcaa gcattcttaa ctcctaagta
    23281 ctctattgat atggccattc cgactcctcc tgcccctgca atggcagtta gacccatagg
    23341 tttattttta tattttaatg cctgcataag atgaaggata ttaataattc ctgagcaggc
    23401 ataagggtgt ccgtaagcta atgtccctcc aaaaatattg aatttttctc tctcttcagg
    23461 ataataatga ttaaatagag catcaatcgc tgcaaatggt tcattccatt caattgcatc
    23521 ataatccgat attttagtat gagtttctgt taatagtttt tccgtagccg tgtgaaccaa
    23581 ttctggacta agcttgggat ctcctgctac ttctacaatg tgaacaatcc ggaattctgt
    23641 tttctgactc tgaagcgtta gaaatgcagc agcatcgtgc attaaacaaa catttccaat
    23701 agtgagcaaa ggtgaatttt ccatcaatct tggtaatttt tgaaaaaatg tttcttttag
    23761 ttttctaacg ccttgatctc gcatcccttc cattggtaag attacctctt ctaaatagcc
    23821 accttgttta gctgttaagg cgcgtttatg gctcaagaat gccaatttat ctaacatttc
    23881 tcttctaaaa ccatattttt gacagactct ctgggcccct tctaacatta cagtttcagc
    23941 ataagagtca ggagaaaact gagcaactgt atattctccg ttacgattat cttctttagc
    24001 ataacgtctc ataggttgaa gagaactact ttcaatcccc ccaacaagaa ctttttcatt
    24061 aataccggta ctgattttta gataaccaaa aaacaaggca gaacttgatg aagcacactg
    24121 catatcaatc gtttgtactg gaatatagga ttcataatca gaaaaaagag tcatcaaacg
    24181 accaatattg cccccagtac caactgtgtt cccacaaata atactatcaa tgttagattc
    24241 tgattctatt ttttttattt gatttaaaag gtgtgctcct aaaagttctg gacggtaatg
    24301 tttaaattgc ttccctttta taccgatagg tgttcgtagc ccaaaaccaa tataaacatc
    24361 tctattcttc atatgttccc ctcaccataa tatttgatgc tgaaaaataa tcactgtgtt
    24421 atccactact tgcactgaga attcttgatt cacccttact ggagataaaa aagtcaaatg
    24481 aatctccttt atttttcctt ttctaacttc cattattttt tccaataata gagccgcagg
    24541 cacaatagct gcttcccctt ggtgaaaagg atttttatct cgcactaagc tagtgaattt
    24601 ttgacattct tctattgaaa taagactagc cttctcttcc cttgcaggac agtcactgga
    24661 tgctagtgac attggcttaa gttttggtaa attaagcatc ctcaaatggt aaatccattg
    24721 ctgatcagtt accgttacct caagaattct cttagaaatc catttaatat tatcagatga
    24781 gccatcctct ctttgataca aactaagttc aacaattaca aaattaagcg gcaaatctcg
    24841 gcacatagct atcgcttttt taaatagttt tttcatctta tctcccgtca aaatataaga
    24901 ccagtatatc ataatttttt atattgttaa cttatgacat cgactaggtt tacaatatag
    24961 tttttttatg ctattattaa tttcgttaaa ggaggctatc atgtcatttc aaacaaatta
    25021 tatccatttg gctgacgaaa tattatctgg aaagactagc atttcttatg aacaagcgct
    25081 ggaaattcta aatagcgatg agaactggtg ggaaatctac gctgctgcct tataccttaa
    25141 aaatcaagtt agccgaaata atattcgcct taacgtttta ttaagtgcta agcaaggact
    25201 ttgtgcagaa aattgtggtt actgttctca atctaaagaa agcactgctg acattgataa
    25261 atttggtctg cttcctcaaa atgtcattct aaaacaggct attgtcgctc atcaaaatgg
    25321 tgctagcgtc ttttgtattg cgatgagtgg aactaagcct agcaagagag aaattgagca
    25381 gttatgtcaa gtcatccctg aaatcaaaaa aagcctccct ctagaaatat gccttaccgc
    25441 tggttttttg gatagagaac aacttcatca gctaaaacaa gcaggaattg accgtatcaa
    25501 tcataatctc aacacccccg aagaaaacta ccccaacatt gcaacgacgc atagcttcaa
    25561 agatcgttgt gatactttag aaagaattca caatgaagac attgatgttt gttctggatt
    25621 catttgtggt atgggagaga gcgatgaggg gctcatcaca ttagctttca gactaaaaga
    25681 gctggacccc tattctatcc ctgtcaattt tttacttgct gttgaaggaa cacctcttgg
    25741 aaaatataac tatttgactc ccattaaatg cttaaaaatt atggccatgt tgcgttttgt
    25801 ttttcctttc aaggaattaa gattaagcgc tggacgagag gtccattttg agaattttga
    25861 atcattagtc accttacttg ttgactcaac ttttttggga aattacctaa cagagggcgg
    25921 tcgcaatcaa cataccgata ttgaattctt ggaaaaatta caactaaatc atactaaaaa
    25981 ggaattaatt taaatgaaaa caagaacaac tacctatatt gcactcatgg ttgcactact
    26041 tattgtttta ggttttatcc cagggatccc attaggtttc attccagtcc caattgtttt
    26101 acaaaatctt ggtgttatgc tggcaggtgc tctgcttggt agtaggaagg gtttcctagc
    26161 tgtggctatt ttcttgctct tagttgctat tggtgcacca tttttaccag gaggacggtc
    26221 aggtctcgta acgctgtttg ggccaacagc aggctatctg ctgacttatc catttgcagc
    26281 tttttttatc ggcctagggc ttgagaaagt gaaaacaaca aaactctggg ttcaattcct
    26341 tattatttgg atatttggcg tactattgat tgatatttgt ggtagcatcg ttctttcctt
    26401 tcaaactact ctacccttaa ctaaatcact attttctaac cttattttta ttcctgggga
    26461 taccttaaaa gcatccattt gcttgattat ttatcgtaaa tttgcaaaca gattaactca
    26521 tctatacaac taaataaaag acagtaccta aggtactgtc ttttatttat agagagtcat
    26581 tatcctacac taacacattt tagtcattaa ttaacgtcaa aaagttttca atcattttca
    26641 ttccatcaat actcccgatt gactcaggat gaaactgcat cgcatatacc tttaagctgg
    26701 ggcaatggaa tgccatgatt tcattatcct catttgatcg tgcaataaca gcaatattct
    26761 ctggtaaatc agtcactacc aatgaatgat aacgcataac ttgggttgga ctatcaattc
    26821 ccttaaacaa actagtatgg tcgtttattg ttacccaact ttgtttacca tgcataacgt
    26881 gatttgctaa atttaatcgc ccacccaagc actcggcaag cgcttgatgc cccaaacaga
    26941 tacctagaat aggtttcttt cctataaact gattgattaa ttctaccatt ttcccagcat
    27001 cctttgggtg tcctggtcca ggtgataaga cgatggcttc tgcgctctca gctaataaaa
    27061 aaaggttagg aacatcattt tttatgacga atacctcttt atagacactt aaatattgct
    27121 ttaaattata ggtaaaagaa tcgtaattat ctaccaatag taacatttta tagacctttc
    27181 cttgcacttt gacctcatta tacactaatt gtaaaatata aaaaaggctt agaggaataa
    27241 ctactccaac ctctaaacct cacagattat gccttttgaa tagtctaaat tttctagacc
    27301 tccatcatgc cagcttgtaa ttggtacata cgataataag tcccttttag gtctaataaa
    27361 gactcgtgat tcccactttc aattatcttt cctctatcaa gaacatagat acaattagca
    27421 tcttgaatgg ttgataagcg gtgagcaatg gcaattgttg ttcgaccttg acgcattttt
    27481 gctaacgaat cttgtacaat ctgctctgtt tccgaatcaa tatttgctgt cgcttcatct
    27541 aagattaaaa ttttaggttt actagccaca gttctggcaa acgccaatag ttggcgttgt
    27601 ccagtcgaaa aacttgaccc acgttctgaa acagctgcat cgtacttatc tggcaacttc
    27661 tgaataaatt gatcagcatc aacaaactct gctgcgtcct gtacctcttg atcggtgatg
    27721 tcttggtaca ttttgatatt agatttaata gtgccatgat ataaaaaggg gtcttgtaag
    27781 acgagtccaa tattctttcg caattgttct tgtgagtaat ccctaatatc cttcccatct
    27841 aataaaactt gtccagattg aaattcgtaa aacctcataa agacattaat aatagatgat
    27901 tttccagaac ctgtcgctcc tacaaaagca atcgtttcac cttttttaac actaaaagaa
    27961 acattatcaa ggatttgttt ttttccatca tatgagaatg atacattttt aaattcgata
    28021 tttccttctc tcacaaaagc ttctgtattt ttttggcttg gttcaaaacc tgtttcatca
    28081 atcaaatcaa acacacgccc tgctgatacc attgatgttt gtaaggttga aaaattttgc
    28141 gttacttcaa ttaaagggtc aaagagacga ttaacgtact gaataaaagc atacatcaat
    28201 cctgccgtaa gacctccttt aactcctgta aatccaaaat aagccataag aacagcatat
    28261 gctaggagtt ttaaaagaga catcgccggt cttaagaaga gactatcaag agccatagaa
    28321 cgattggcat acacaacatg ctctttgtta atttcctcaa attcagtctt caagcgctct
    28381 tcttgaccaa aagcctgtac aatgcgaatt ccttcaatac tttctgataa tttactgttg
    28441 atatcactaa gtaaacttct cgttttagca atgacagtga ctgatttttt ccgatagaca
    28501 ttcactaata taaagataac aggtaataaa agagcgacaa gtcctgttag tttaatgtct
    28561 agcatcaaca tagtgtacag agtaactgta aaaataaata tcgccgagat aaaactcgat
    28621 aaaatacccg aaaacatatc agatattgct tcagtatcat tagtaatacg tgacactata
    28681 gatcctgccg gtgtcctatc aaaataagac atgccaagcc tttccatatt agcaaaagca
    28741 tctctacgaa tatctctaac aatactataa gaaacacgcg caaaaaagag attcccaaaa
    28801 tattgaatta aggtctgcaa gacatacatt gaataatatc ccactaaaat aagaactgct
    28861 gtttgattaa catttgtcag atagtgatca ataaaatgtg aagcaattaa aggaataata
    28921 tttttaacaa cagtcgtcaa caataagaga gatagagcta atactgtaaa ccatttataa
    28981 gggcgtaaat aggagattaa tctcttaaag acttgccatt gattagactt catcaaatgc
    29041 ttcctccatt tctaatcgct gtgaggcata cgtttcagca taccaaccac ctttatttag
    29101 caactcttga tgctgacctc gctcgatgac tctgccgtct tgcataacta agataaggtc
    29161 tgcgtgcaca acagctgata atcgatgtgc tgaaataata gtcgatttcc cttgacgatt
    29221 cgttttaaga ttttcaataa tggcatgttc cgttttagcg tccactgctg atagagaatc
    29281 atccaaaata agaatatctg gatctaaaat catggcacga ctcatcgcaa tcctttgttt
    29341 ttgtccacca gataatgaga ctcctttttc tccaattaga gtctcaaatc ctgctggcat
    29401 ctgtttaatg tcatcgtaaa catgagccaa tttagttgct tttttaacag cattgataga
    29461 tagagttgga tttccaaagc gaacattttc taaaatactg gtagcaaata aaaactgatc
    29521 ttgaggaaca taaccgatta gttgacgtaa ctcagacaat cgataatcac gtatatcatg
    29581 tttatttaaa gtaatttttc cctgagtcac atcatgttca cgtagcaata acttaataag
    29641 acttgtcttc cctgatcccg tttgacctac caaacctaag gtttgacctt tttctaaggt
    29701 gaaatgaata tcggctaagg tttcttcatt gtcgtatcta aagaaatcaa tatcatatct
    29761 taatgttcca ttgacaacag gtctgatagg atttaaaggg tcagttatat ccgattcttg
    29821 ctcaagtagg ctattaatac ggttataaga aacactacca cgctgtacca tattgaacaa
    29881 gaaaccaatc gccatcaagg gccataccaa catatctaaa tacgtcacaa atgttaccaa
    29941 gtcaccaaca gtaacttgac cttttgaaat cataaaagct cccatagcca atgttaatac
    30001 gtaggaggca cctataaaaa gaagaactaa aggatcaaac atgacatcat aagtcatggt
    30061 ccgcatgttc ttcacgaaag tcatctgatt aacctcttga aaagaagcaa tttcttgttc
    30121 ttgataacca aatgatttag tcactttgac gccagagaca ctttcttgca ctttattatt
    30181 taattctgaa aaggctgcct gagattcttt gaaggtttca tgggtttttc gccccaattt
    30241 actagttgct aaggccatta agggcaaagg gataaccgca attaatgtca tttgccacga
    30301 aatagtaaag aacatggtga taagcgttac taatgctgtg atagaggcat ccactgctga
    30361 cataactcct cctcctgcaa gacgtgttag agaattgatg tcgttggtcg cgtgcgccat
    30421 taaatcacct gtacgatatt tctgataaaa agaaggagac atttttgtaa aatgttcaaa
    30481 taaacggtat ctgacaactt gacccaattt gtaagaagtc cctaaaatat acatacgcca
    30541 aatataacgc agcccataca tagctaaagc tgacaaaacc aaacctaata aattccagag
    30601 caattgtggt cttgttaatt ttccagttgt aatagcatca ataactgatc ccatgatttt
    30661 aggagggata agatttaaca ccgcaaccaa acttaatgac aaaattccaa taagatagcg
    30721 cttcttttct tccttaaaaa accaccacaa attttttata atagacatat ttttcctttc
    30781 tcaaagattg aaaaacatca taattaagtc agaagacatg aaaagtgttt taaaattctc
    30841 atttaacaac tctctattat gcctctataa gcttactttc gtcaactaaa gtgattcttt
    30901 ttaaatagta aaaggcttga acaatatcat agcctttaca ttattttcat tttctaatag
    30961 ttagaagcca accttttatg tttcgataag tccatagggc gaaaggaccg cctaaggtat
    31021 ttgtccatag atcgtcgatt tcaaaaaccc gattagcatc tattaaaata tctaaaacaa
    31081 cttgagtaca ctctatgaaa atagacatca agaaagcaag taatataacg cttttatatt
    31141 tccgtaaact tggctttagg gatagtaacc ctataatgag aggaaacagc agtaaaatat
    31201 tcactacatt ttgaccaata acccaaaaaa tctctttaaa gctagttagc tgatctaaac
    31261 tacgaaaaga attaaatggt actaaaagag ctacaattct tccgaagcgc tgaacattcg
    31321 gagttgatac cccctcaatc ataatttgag gcccaaaaca catcctgaca ataataagac
    31381 aatagataat cactaacatc caaacgaaac gacgactaat ctttgtcaat tccccatctt
    31441 cagtaagtaa tcttttaagc attactgccc taccttcgct ttttgacgat cgcgtttcat
    31501 tgtgtttgaa cgtaattgcc cgcaagctgc atcaatgtcc gtaccatgtt cttgacgaac
    31561 aacacagtta actccatttt tcttcaaaac gtcatagaaa gcttctacac gctctttagg
    31621 actacgacta tactggtcat gttctgagac aggattgtaa ggaattaaat tgacataaga
    31681 taatttgcga atctttttcg ttaaatctgc taactcttga gcattttctg gggtatcgtt
    31741 gacaccattt aacataatat actcaaatgt tacacgacgg ttagtcgtct caatataata
    31801 ttcaatagcc gcaaacaact tttcaagagg gaaagaacga ttaatacgca tgatacttga
    31861 tcgtaaatcg ttatttggag catgaagaga tactgctaag ttaacttgta caccttcatt
    31921 tgcaaattca cgaattttat gtgctaaacc tgaagtagac actgtaatgt gacgtgcacc
    31981 aatagctaat ccattatcat cattaactgt acgcaagaat tttaatacat tggtatagtt
    32041 atcaaagggt tcacctattc ccatgaccac aatgtgacta acgcgctcac cttgtccacg
    32101 ttcatcaaaa tatttttgaa caagcataat ttgagcagtg atttcgccat tgttaagatc
    32161 acgttgtttc tttattaaac cacttgcaca gaaagtgcaa ccaatgttac aaccaacttg
    32221 agtcgttaca catactgata aaccataatg ttgacgcatc aaaaccgtct caatcagcat
    32281 gccatcaggt aattcaaaaa ggtatttaac agttccatca gcagattctt gaacaatacg
    32341 ttgttttaga ggattaacaa caaaattctc gttcaaaagt gcaataaaat ctttggaaat
    32401 gttagtcatt tcatcgaagc tttgcacacg ctttttatag agccagtccc agatttgtga
    32461 agcacggaat ttcttttctc catgttcaat agcccaagct attagttcat cgcgagttaa
    32521 gctataaata gagggtttat aggcgggtgt atcagttttt ttaggcataa tattttcttt
    32581 cttcatggtg gaataaacgg ttacagttta tccctttctt attttttctt acgaataaca
    32641 aaatgctgct ttgcagaggt catttcctta tcttgctctt ctttaatatg acgttcttga
    32701 cggcgttttg tgtctttacg ctttttactt gtaaaatgag cattggcatt tgactgggaa
    32761 cgattatttc tagtttgtga ctttgatgat tgtgatttgg atcttgactt acgacgctta
    32821 tgaggtcttt catcatcaaa tttaatatct ctaggatttg gattctccaa cacaaaataa
    32881 gcacaaccaa agttacaata ctctttaata taatcttcta aacgtgaaat ccggctattt
    32941 tttctaattg tactagcatc tttgtaaaag cctcttagcc tcaactgctc gttaccccaa
    33001 tcgccaacga taaaatcata ttttaataaa atctcagaaa aacgttgtcc aaagacagtc
    33061 acatcaaaag ctgatttttc attaataaca agttgaaatt caatatcatc acttttaacg
    33121 atattttcaa agtgaatgaa ctgtgggcca ggatacttat tatagttaag catctctggt
    33181 gtcacttctt ttcgcataat actcctttct ctaaaatcat tgagagaaac gactagcttc
    33241 tactctttat ttatctattt ttcatttata gtgtcatatt tattaaataa tctgctgcca
    33301 catctctgag taattcagga aaagcaagtc tacttttttc gtttttaaac actcaaaata
    33361 cgatgcaaaa taatactgat caacgattac aagaatcact tcttctttgg catcaatatc
    33421 tttctcatta tacactattt ttccattttt gtaatcaaat cccgaatgta aaacttctcc
    33481 aaagcacttg ccacgaaagc ccataccatg aatcttttgt tgcgctaaaa attgaccttg
    33541 ttgataaata gtttcataaa tctctaaaag ttcttgacta ctcactgtca atttagcgag
    33601 gcgcatttga tgaggtaatg attcgtggag atttttcctc gaaaagtctt tttcaaatgg
    33661 ctgaacaatt aagccagaat tgataatagc aacatctgca cctgcgtaaa acagcatcgc
    33721 atccatgact agttggtacg attcttctaa tgataatggt gctggaaagg atattaaact
    33781 ttcttttcta agctgttctt taccttctga ctcaatcttc ttagccagtc actgtccgaa
    33841 ggataaccag ttagttgttt tgtatcacat gttgagatta aaatatcttt tagtgtatga
    33901 ttatcaaaag ttatatcaat actcccaaca aagcgaccat atttaccagc tgctgcaaga
    33961 tatgtaccgt taattagctc cccttcctca aataaatggt gtgtatgtgc acctataata
    34021 aggtcaattt ctgaaaattc ttgagcaatc cttgtatcaa aacgaatacc taaatgactc
    34081 atcaaaatgc gacaatttgc ctcttttatc tcatttattt gtaaatggca tttaagacag
    34141 tcaattggat cttctatcgt ccacccatta ggttcatagg ttttataata aggaaaagta
    34201 taagctaaaa aggcaagctt agttccttgc tgcgtctcat aaataatgta aggttttgcc
    34261 caactcggtt cgattatgtt atcttttaga ttgccaacaa taaccgtaaa atctgaatct
    34321 ttgtagactt ggtccaagtc ttttttggat aaaccaactc cttcattatt accaatagta
    34381 gcaagctcaa tacccagttc attcatcaat tggacattag cctttcccga gcttgcttct
    34441 gtcaaaggat ggcttttatc aatattatcc cccaagtcaa gtgaaatcgt ctctattggc
    34501 tgcgcttgat tgtcatgaaa aaatcgtttt accttaggaa aattctcaaa atgagagtgc
    34561 agatcattta aatgtagtat tcttataagc tctgtcatac tatcatttta gcactttgat
    34621 agtcaaatgt cgcaaaacaa aaaagccgag cagacgcccg aactctttga gtttattttg
    34681 tttttttcaa ataatcaata gcttgctgaa cattttgaac tggtacaatt ttcatcttcg
    34741 tacctaatcg cttagctgct gcttttgcct cttgataatt cgtctgaacc tttgttttgc
    34801 cttttttagc atttttatcg atcggattat taggaacaaa gaaaatatcc attccttttt
    34861 tagccgctga aacaactttc aaacctgctc caccaatatc gccaacatgc ccattttgct
    34921 caatagtacc agtacctgca atcttacgtc ctttacgtaa atcttcttta accaattggt
    34981 cgtaaatcgc taaagtaaac attaaacctg cacttggtcc tccaacacct tcagtattaa
    35041 agtccacagg aacatcagat aatacttctg tatggtctgt caaaccgata ccaataccat
    35101 ttttaccgtt tgatagttta ataactttac caactgattc tttcttttta ccttgacttg
    35161 tgtattgtac ctttacttta tcacctagat gaagagctgc aacatatttg ataagttgag
    35221 aagaattttt gaaacttttg ccattaactc ctgtgactgt atcagctaaa tgtaatctat
    35281 ctttaaatgt cgaattttta gctaagttaa gcacataaac tcctttataa gtaagagata
    35341 cttgcttatt agctaacttt aaagcttgat agatggactc attttgagaa gtctccatgt
    35401 agaattgatt aattctgaga taatcgtcat tactaaagcc acctgttgtc tcctctttac
    35461 tagataattc tgtaaaagga gttaaccaag cataaagaac ctgagctggc gttgcttgac
    35521 tgacagatac cgctacaaaa ttgtacgaac ccttagcttt atcagccttt ttattcactt
    35581 tcaataccga acggatatca taagctccac ctggcatttc caaataataa ggtagtctca
    35641 ctacaaggct tgctaatact aacaataaaa aagcaaaacc tataatccac catttaagca
    35701 gacctagtaa acttttatgt tttctttttg gatctctatt cttcactcat tttctccact
    35761 tctctaacaa cactctttgg aacaaaaggc ttcacagaag ccttaaaatg tatcaattca
    35821 cggatgcgac tagatgatat cggactcaaa gatggcgacg ttgataaata aactgtctca
    35881 atatcatctg ccaactgttt attaaaaaac tccaaattgg cttcataatc aaaatcctgg
    35941 ctattacgca gacctcgaac aaaatatttt gctcccactt cccttgctaa gtcaacagcc
    36001 agtctatctt gcgccactaa aactgaaaca ttcttaaact gacggatagc ttcttctaac
    36061 atttttttac ggcactcaat cgaaaagtaa ccttgcttct caaggttata aaataaccca
    36121 atgtaaacat gatcaaatag atagctggcg cgctcaataa tatctaaatg tccattagta
    36181 acaggatcaa atgaacctgt aaataaagct ttctttgtca ttcgtcgtca tctccacaat
    36241 aatacaacca taaaaagact accacaggaa tcaggcgaac aatgtctatt gcctcaaaac
    36301 cattagctat ccaattgata agatttggca aaaaagtaat cattgccatt aatagataca
    36361 atggattttt tgttcttgat aatgaacaaa ctccttcatc atttttgaag tgataatgtt
    36421 ttagcaactc ttctaaatag tgaaggcgca acaacaaata aatcaatatg actaagatga
    36481 gaataatcat cactaacttg taatccatac caaagcctcc taacgaacgt agaccgttac
    36541 tttagaaatg ccataaattt tctgtttcca tatcccaaaa gaagcaattt cttcaggtaa
    36601 atcaactgat ttgtccgttt cacaagcaat catgatatca tcgcctaata aacccttgct
    36661 atccatgatt tggatttgct ttacaatttc ttccttagca tatggcgggt ccaacaagac
    36721 caaatcaaat tgtcccgtta attgttctaa agcacggttg gcttccattt ttaataattg
    36781 aaattgctcc ggactcttag tcattgcaat attttcttga ataacgacct gcgcacgcct
    36841 atctttttca actaggacag cttggtccat tccccttgag atcgcctcaa tagctaagct
    36901 accactgcca gaaaaaaggt ctaaaacacg accaccttca aaaaaaggac caatcatatt
    36961 aaaaatagcg cctttaacct tatcagttgt tggacgagtt gtttttccat caagcgtttt
    37021 taaaggacgc cctccaaatg ttcctgctac tactctcata accactatta taacaaaaaa
    37081 taaggtagag ttatcaaaaa attgcttata acaaacagag aagatagtat ctcttttcct
    37141 tcttttttga tatgataagc tagcagtaag agaaacgagg ttcccataat gattaaaaaa
    37201 ctattattaa ctttaatagc catattatgc cttacaacgc taaaatcaaa cttaagtagc
    37261 gctgacacca tgactatttc tcaacaacaa taccaacaca tgataacaaa tagtgactta
    37321 atcgccatca cagataaggc gttagtaagc aaaagtcaac aatctgaagc gactattttt
    37381 ataggacgtc caacttgcca atattgtaga gctttcttac caaaattatt aaaatctcaa
    37441 gccacacttc attcaaaaat ttactacctt gatagtcaaa aatacaaagg gaaacgattg
    37501 aagagttttt tcaaaaagca tcatattacc actgttccaa atttagcaca ttatcaacaa
    37561 ggaaaaatga ctaaatatct tgttcaagga agccaagcta cacctcaaca aatccaaact
    37621 ttcttaagcc aaaaataaac ttattgttaa aatcagccaa acaaaactgc ttggctgatt
    37681 ttttataata tattttcaaa ggtatctctg acttcttttg gccagacact tgactgtact
    37741 tctccaatat gttttttacg aagcaagaac atagctaatc gtgattgacc aattccccca
    37801 ccaatagtca gagggaagaa accacgtaat agcgtcttat gccactcaaa ctctaaacga
    37861 tcttcatcac cggtgagaac aacttgacgt ttaagagcat cttcatcaac tcgaattccc
    37921 attgatgata gttcaaaggc agttccaagc tgttcgttcc acactaaaat atcaccattg
    37981 agacctttaa aaccattttc tgatggtgtt gtccagtcat catagtccgg tgcacgacca
    38041 tcatgtggtt taccatctgc taactctcca ccaataccaa tcaaaaagac tgcgccatac
    38101 tccttagcga tggcattttc acgctcttta gggcttaagt ctgggtattt ttctaccaat
    38161 tcttctgtat gaataaaggt aatacgcttt ggtaaaatgg attcaatatc aaaacgagct
    38221 tccacggcaa gctctgttag gcggatagct ttgtagattt tttccacagt ttcttttaga
    38281 tagtccaaat tacgtctacc atcaggaatg accttctccc agtcccactg atctacgtaa
    38341 actgagtgtg ttggatcaag agaatcttca tctggacgaa gagctttcat atgaacaaaa
    38401 agaccttctc cctcattaaa cccaaatctt gccaaagtat ggcgtttcca tttagccaat
    38461 gagtgaacga cttcaaattc agcttcaggt atattaagga ctttcaccga tacaggatgt
    38521 tcaataccag ataaattatc ttgcatacca tccccgacct gactaagaat aggtccttgc
    38581 acttcgacga tttctaactt atcaatcaaa tattgtgtaa aagtattttt gacaaaagaa
    38641 atctcctgtt gctgatgaat aaagcttttc ttcatatgtt cctccttaaa ttataaaatg
    38701 ttagagctat tatactcctt caagtaacaa aatggaagag gaattgtaga attattctga
    38761 cttttttaca agttttttct gaaagtcatt actctagaat tgtttttgcc atgacttgtc
    38821 gcaattcctt ggtcattcgc cgtggtcctc tcacagtctt aacgccgtgt tctgctaaaa
    38881 atgctacagg ataggtagaa tcttcaaatt gccttatcag tttgagtcgc atataagctt
    38941 tgacatctct tgctcctaag taagtacttg ttttaaggtc acgttctatc acttgacaga
    39001 catagcgcaa agcacgaata ggtgctgtaa tataaataaa cacataatct cccaccgcaa
    39061 tctttccttt ttgagtccaa tcaatcaatt gatgctctgc aaactcctta tcaatatcgt
    39121 aaaatttagg attagcagga ataatccaaa actctggctc agaatttgat tttttattgt
    39181 gagaaacaag cttacgacta tctgatacca atttaaaaat ttctatatca gacaaagtat
    39241 tgtctaaaac gatagatacc caagtctttt tagacatatg ataagaagga taaatacctt
    39301 ctttttgtaa aagtatttcc atatcttgag gattgacttt aatattcaaa acttcaacct
    39361 catcttctga cagttgagct ggaacatttt ctagtttccc catcttcaat ggaaaaagca
    39421 gagcatacca tttacctgac acacgatagg aagcatattg tgggtgcttc gcaaatggat
    39481 aatcaactga atcttcaaaa gttttagcta tcttctccgc taaacgatta ctttgatctt
    39541 tggtaaataa taagtcctta caacaagaat ctgaaatatc acctaatata gccatataag
    39601 ccgatcgtac ttcgccaaca aacgaaccat tataatttgc tgctcgaagc ggtaaatatt
    39661 cttcttcaag tgccgaatca ataaccttac cagctatctt tccatccaat gaaatcgtga
    39721 taatagcttt aaagtcatta tccataaatg tttgacaata gatatactct tggtcagatt
    39781 tgataaatcc aaactcctca agcgatgaaa aaataaaacg ttttttccgg aaaaaatctg
    39841 attctattga catagtaacc tctctattca ctgtacatta tcctctatta tgtcgtttta
    39901 agagctagaa agcaagctca aagtcaaaga tgaattctgc cttgcgctaa agtttcttcg
    39961 tacaaaaaaa gcatgttacc atgcttaaaa ctaaactaat tgatagtacc ctcttattaa
    40021 ttctctattt atgtttggga tgattatcct ctttatggac ataaatgtag gtagtgtaaa
    40081 atatcttgtg taaacacaaa aaggaataaa tcctgtatag tagagttgcg agacttcact
    40141 agaaagagat ttattcccat gacccagttt accacagaat tacttaactt cctagctcaa
    40201 aaacaagata ttgatgaatt tttccgttcg tctcttgaaa ctgccatgaa tgaccttctc
    40261 caagtggagc tatccgcctt ccttggctat gaaccatacg ataaggcagg ttacaacacc
    40321 ggtaatagtc gtaatggtgc ttatacacgt cgatttgaga ccaagtatgg tgttgttaat
    40381 ttgttgatcc ctagagatcg aaacggggag ttcagtccag ccttaatccc aagttacggt
    40441 cgtcgagaca atcatttaga agaaatggtt atcaagcttt atcgaactgg agtcacaaca
    40501 cgtgaaatca gcgacatcat tgagcgtatg tatggtcatc attacagtcc agcaacagta
    40561 tctaatatct cgaaagcgac acaggaaaac gtggctagct ttcatgaacg ttccttagaa
    40621 gctaactata ccgtattata tcttgatggg acttaccttc ctctgagacg tggtacagtt
    40681 agtaaggaat gcatccacat tgcactaggc gtcacatcat atgggcataa ggctatcctt
    40741 ggatatgaca tcgcacccaa tgaaaacaat gcttcgtggt cagaccttct agaaagattt
    40801 aagggtcaag gtgtacaaca agtctctctt gttgtgagtg atggttttaa tggacttgat
    40861 cagcttatcc agcaagcctt cccaatggcc aaacaacagc gttgccttgt ccatattggc
    40921 cgaaatattg caagtaaggt gaaacgagca gatcgtgctc taattttgga gcagtttaaa
    40981 acaatttatc gcgcgatcaa tgtagaggaa gcaaagcaag ccttagatag ctttatcaat
    41041 gagtggaaac cacactacaa gaaggtaata gagaccttag aatcaatcga aaatctattg
    41101 atcttttatg aatttcctca tcagatctgg ggaagtatct actcgaccaa ccttattgaa
    41161 tcacttaaca aagaaatcaa acgtcaaacc aaaaagaaag tcgtctttcc caatgaagaa
    41221 tccctagaac gctatctcgt cacactattt agtgattaca acttcaagca aggacaacga
    41281 atccacaaag gttttggcca atgtacggac acacttgaaa gcctatttga ttaacaatat
    41341 cagctttgct agagtgttta cacaagatta ttgacagact ctaaatgtac ataacaatag
    41401 agagcataat agtaatgata gtcgtaatta aaataatcaa tacccagaca gcatccacac
    41461 tatcaaaagg caacttaaca ttcattccat aaaaactggt aaccatagca ataatggaaa
    41521 taccaatgga aataattgtt aaagtagtta cattgtcgtt aaggttattg ctgaggacat
    41581 tattataaga agaaagttct tggaaaacct gtgaatttaa ggaacacatg ttagataatt
    41641 gtcttgcttc aataatagca tcttgaagtt gcatcttttc atttctagtg ttctctttat
    41701 cttgctcaat gttttgtaaa tcggtcagca tttcaaaatt ttgcttagtt cccatcatta
    41761 agtgtactgt tccactttgt aaatttgcca gccttgccaa gttcgatttg tttggtcttt
    41821 tacgcaaatc attgattagt ttatctcttt cttcagaaat agtatctaaa acatcaaaat
    41881 attgctttga gaaagcagtc actaatgata ataagaggtg tttcggagaa tctacctttt
    41941 caacacgatt aagtgctttt tttacaaggt taatattttt atgattagct aatacaaaca
    42001 agccatcttc tttcaaaaat agagtcactg gtatcactct cggcacatga cgaacatcat
    42061 caacgtgacc ttgcgtatcc aaaagctggt aaacaagtgc caaatgcccc tcaatatcac
    42121 gtacgaaaga agactcattt tcatcacgtg cataagccag caattctgaa ggtaaaaatc
    42181 tctcctcttg caaggatagg tcgtcaacat taattatctc aaatccaaaa tcgctcgaaa
    42241 acatagaagc acctcactct aaacttgtct acattatgaa ataaaaatcg agaaaaaaca
    42301 agtagaaaac ccttttaaat ctaattaacc ttatgagagc aaaacaaaaa aggactacta
    42361 aaataacatc tagtactcct tattaatatg ctagatagcc agcatcattt cccccatgaa
    42421 cggtataagt cttctataat cttaacacct gacagcgtca actgaggcgt catgatatga
    42481 cttacccttt gcccttctaa aatcatttga ctaacatgat aagcttcttt ctcaaaatca
    42541 ctaaccatgt caacttgaat cgttcgcgca ctagtatcat ttctcaccaa ttttgcatga
    42601 gtggttttcc aaaaatgtgg aatgattaga cgaccttcag ttccataaat aatcatttca
    42661 tgcggtaaat ttagacgtgt tgtcaagaag atatctacga ggaccccatt tgataattgc
    42721 aagagtagtt tggattgact atcgctttga ccttttggaa aagtcgcagt cccactagca
    42781 tgagttatag tcgcatcaaa tagatattgt aaataagaca gggcgtaggg cgccataaaa
    42841 tgcactgttc ctccacctaa ctcaagttct ctaaaccatg ttacatggtc aatgtttggg
    42901 taggctgttg tagatgagat ggagatcact tctcctattt caccagaggc aagtaatttt
    42961 ttaatcactt gtgtcatcgg aataaatacc gatttttgtg cctccatcaa aaacagatta
    43021 caactttctg ccaaagcgaa taattcattc gcttggtcgt aagtcaatgt gaaaggcttt
    43081 tctaccaaca catgttttcc cgctaacaga gctgctttag caactttata atggtcttga
    43141 ttaattgttg ccacataaat cacatcaatt gactcatctg ctaacatatc ctctaactta
    43201 tcgtaagcct ttgggagatg atatttgttg gcaaaagctt gcgcagactc aagagttcga
    43261 ctcgatacag ccacaacttc accatttcct gctaagcgga ctccttcaat aaaacgaggc
    43321 gccacctttg ccgttgacac aacaccatac cttacctttg acataaaaac tcctttgaca
    43381 ttgatagagt tattatagca taagaactag catcaaacag tttaaagcga ctatacataa
    43441 taaggctgca caatttctac ttattatctc taaagatcca taatcaaatt atatcaaaaa
    43501 acgccatcat ttatgatagc gtttttttat ttttttacct caccaacttc ttcatttctt
    43561 caatacgtgc tatggttgcg tcgtatttag tttggtagtc ggtctgttta tctttttctt
    43621 tttggacgac ttctggttta gcgttagcta caaagcgctc gttgctgagt tttttgccaa
    43681 ccatatctag ttctttttgc catttagcaa gttctttttc aagtcgtgcc aactcttctt
    43741 caacgttaag gaggtcagca agtgggagga aaatttctgc acctgtgatg actgatgaca
    43801 tggcaagttc tggtgtggca attgctgagc tgatttcaag cgtctctggg tttgtgaagc
    43861 gtttgatgta gttgctattg tctttgaaga agctttcgag ttcgctgtca cttgtcttaa
    43921 ccaagatagt gattggttta gatggagcaa cattgacctc tgctctgcta tttcgcactg
    43981 aacgaatcaa atctttcaag ctttcaacgc ctttgtgagc tgtttgattt tcaaatgtcg
    44041 cgttaacttg tggataactt gcaaggacaa ttgatccttc tgcatattgt ccaaaaattt
    44101 cttctgtcac aaatggcatg attggatgaa gaagacgcaa gatttgatct aaggtataaa
    44161 gaagaacaga gcgagtgatg actttctcgt cttcattgtc actataaaga acctcttttg
    44221 ttaactcaac gtaccaatta gcaaattctt cccagataaa gttgtaaagg atgtgaccag
    44281 caacaccaaa ttcaaacttg tcaaagtttt cagtaacttt acccacagtc tcatttaaat
    44341 tgtggagaat ccaacggtct gttacatttc caacttgact attaacaact ttttccacat
    44401 tttctctggc ttgatccaac gttaaacctt cgttattcat aaggatatag cgtgaaatgt
    44461 tccaaatctt attaatgaaa ttccacgaag catccatttt ttcgtatgaa aaacgcacat
    44521 cttgaccagg ggctgaacca tttgagagga accaacgaag ggcatccgca ccatatttct
    44581 caatgacatc catcggatca atcccgttgc cgagtgattt agacatttta cgtccttctt
    44641 catcacggat gagtccatga ataagaacat ttgagaatgg ttggcgtcct gtaaattcaa
    44701 gggattggaa aatcatgcgc gatacccaga agaagatgat atcgtatcca gttaccaagg
    44761 ttgacgttgg gaagtagcgt ttgaagtcag ctgcttcggt gtctggccat cccattgttg
    44821 aaaacggcca taaagcagaa ctaaaccatg tgtctagaac gtcttcatcc tgtgtccatc
    44881 cgtcaccctc tggggcatct tctccaacat acatctcacc attgacgttg taccatgcag
    44941 gaatttggtg tccccaccat aattgacgag agataaccca gtcatggaca ttttccatcc
    45001 agctcataaa agtatcgtta aatcgtggtg gataaaactc taccttgtct tctgtatctt
    45061 ggttggcaat ggcattctta gccaattgat ccatcttgac gaaccattga gtagataaac
    45121 gtggttcaac cacaacgcct gttcgttctg aatgaccaac agaatgagtc gtttttttaa
    45181 tttttactaa gtttcctaac gattctaatt tagcgactac tgccttacga gcttcgaaac
    45241 gatccatacc attaaattca tccgcaagtt cattcatagt accatcatcg ttcataacgt
    45301 taacttgtgg taagttgtgg cgttgaccta cagcaaagtc attagggtcg tgagccggcg
    45361 taatcttaac cacacctgtt ccaaattctg gatcagcgtg ttcatcagcg acgattggaa
    45421 taggtttatt gatgatagga aggataacat tttgaccaat caaatccttg taacgtgcat
    45481 cttctggatt taccgctaca gccacatctc caaacatagt ctcaggacga gttgttgcta
    45541 cttctagggc gcgtgagcca tcttccagca tataattcat atgatagaaa gcaccctcaa
    45601 catccttgtg aataacttca atatctgaaa gggctgtgcg agctgctgga tcccagttaa
    45661 tgatgaactc cccacgatag atccaacctt tattgtacaa atcaacaaag acttttctta
    45721 ccgcttttga aagtccctca tcaagggtaa aacgctcacg gctataatca accgaaagac
    45781 ccatttttcc ccactgagat ttaatagttg ctgcatactc gtctttccat tcccaaactt
    45841 tatctaggaa tttttcacga ccaagatcat aacgtgaaat gccttgctca cgaaggcgct
    45901 cctcaacctt agcttgagtg gctataccag cgtggtccat acctggaagc cagagtgtat
    45961 caaagccctg catacgcttt tgacgaataa tgatatcttg tagagttgta tcccaagcgt
    46021 gacccaagtg caatttaccc gtaacgtttg gtggtggaat tacgatagaa taaggcttag
    46081 cctcagtatc ccctgatggc ttgaaaacat cttggtcaag ccaagtttga taacggcctt
    46141 cctcaacctc ggcaggattg tattttggtg atagttcttt tgacattagt ttttctcctt
    46201 gatttggttt attttttgaa taataagata tgctgtttca gtagcagtta agcaagtatt
    46261 attgatttta tgataatatg taagcgcttc tggggctact tcaggattaa agatagcgta
    46321 atccatggtt gaacaaatat cgctctctga ccatttgata tcacgttttg aaggcttatg
    46381 cttgaggcga ttttctgtgc gatttcgttt gagacgctca cttagctctg tttctaattc
    46441 tacaaataat acctcttggt tgtgagattg aaaaactatt tgaatctttt ctagaaatac
    46501 tacatctctt gaatcattga aatcaattac aatagtaaaa atcatttctt gaccagtctt
    46561 agcaaacgtt tcaaaaaatt taaagcgaat agactccgtt agagcaatac tatctggaga
    46621 ccatggcatg aagcgtaaaa caaaatctat agaatcatgg ttatggaata aggtcatgcc
    46681 tgtctgtttt gcgatttcct gaccaattgt cattttccca gaagcttgtg ctccgatgat
    46741 aataatattc atcttctttt tttctcccac tcgcttcgca gtaaaccaaa gcgcatatcg
    46801 ccacaacgtt tcccctgggc atctcttcga tcacgaatat tagcctctaa agtaaaacct
    46861 aatttgcgcg caaccgcctg actttgctta ttatagtcat agcatcccag ctcaatctta
    46921 tgtagtccta gcagtgtgaa acctatctcc acaagggctg aagctgcttc tggcacaatg
    46981 ccttgtcccc aataatcagg gtggagcagg tagccaattt caaaaatgtc atcttcgtgg
    47041 cgatggttaa aatctactga accaattacc ttatcatcgc cttttagagt aatagcataa
    47101 ccagagggta acttttcttt ttcaagatta gttggataaa tctcttgaat ataggttatc
    47161 tcttcttcca agcttttaac agccggaaaa cctgctggat aactcacctc atcaagtttg
    47221 acaaattcaa atatctcctt agcatcttct aacttgcgtt gacgcaacaa cagacgattc
    47281 gtttccacaa caggtagctg gttatctacg atttctccaa gtatcttaat catttgtctc
    47341 ctccatatct acgatattat tgatgactta gttaagtgcc ctgcacagcc aaattagtcg
    47401 atctagtaac ttctatgaag agtagaataa ttactcccac tcactcctca gcaaaccata
    47461 tatcaaattg acacagcgat tgtcttgatt atccttacga tcacgaatgg tagcttcaag
    47521 ggtgaatcct agtttttctg ccacgcgccg gctttgctta ttgtaatcat agcagcggat
    47581 ttcgattttg tggagattga gaagggtaaa accgacttca atcaaagcgg cgacagcttc
    47641 tgtcatatag ccatgacccc agtagtcagg atgaagcaga tagcaaatct cgaaaacatc
    47701 gtcttcatgg cgatggttaa aggcgcagga tccgataata cgatcacttc ctttaacggt
    47761 aataccataa ccagatggta attttgcctt ttccagattt tgatagtagc gattttcaaa
    47821 ataatcgtac tcatcttcta aagaagctat tgggctgagt cctgctggat aggcaacctc
    47881 ttccaagcaa acgtaatcaa acatggctgg gacatccccc accgttcgct gacgcaagat
    47941 aagccgatcc gtttctagtt ttggtaatgc tttgccatcg aaatcttcta gaatctgcat
    48001 atacttcaat ctcctcactc ctaaatacaa aaaatccctg tcaattctga cagggacgaa
    48061 tttcgtgtta cgatttccgc ggtaccaccc aaattcggat gataacatcc gcaactcgtt
    48121 ttgtttcaat tattggactt agcaacaagc tttgacattt gtgcggattt ctcagtatga
    48181 ccgcttcctg tgacaaatgg gatttcaaaa cacctctaag ttaatttatt ctagcaaaat
    48241 aaggctactt tcgcaaggta aatcaaatta ttttgcaaaa acaacaccat tatcaactaa
    48301 ctgtttaata gctgctggac caataccttt tagtgctaaa acatctgctt cacttacttt
    48361 cgcaaatgct tcaaggctga caaggccagc ttcttttaaa acatcttgtt tattagcagt
    48421 taaaccttct aaaagatcga ctgcctttgg cgcttcagga gccgtctttt tcggtaatgc
    48481 gacttcttgt aaagtttgag atagtcgaac atccttccaa ttgatccctt tagctttcaa
    48541 aagctctctt tttttattcg gtttaatacg ctttgccatt gtataatgct ttctcctcat
    48601 ttatgaaatc tatcatagta tctaaaaact actataacaa tgtatcatag aaatgcctat
    48661 ttgacaagct ttctagcgat aaacctttta ataatgacgt tgtccaaaaa gaccatcagg
    48721 caaatcatgg aaagattttt ggttatgata gttattaaat ttcccttgca aaaagcgata
    48781 agcgtctaac ttactctcat aacgaatata cgcatctgct gctcgctcat ccttatcttc
    48841 ctctaaaact ttctttgcac tgtccattgc catttggttg atacttattt gcgattgtaa
    48901 ccactcttca aagtctttta aaaattcttg ttcgtatgac atgatattcc tcttttttag
    48961 tttttttcat tataacatat aaattaaata ctcaaaaaat cattatatca aaaataaatt
    49021 atgatttttc tgacagatat aaaaacaact agttttatct aaggaagacc ttttgagatg
    49081 tatggttctc tatgccattc tagttttggc atcacacccc tagttaaaag aaaacgctct
    49141 ctaaaattca tcacttatac cattcaatca ctattactcc ttgttatctt agcatctatg
    49201 tgttataatt aagaaaaaat gatattacta ttaaaagaaa gaggtgatct tattgccaca
    49261 taaagaaatt ggacatcaat tcttagcacg actaggaaaa aacgcttaag accaggaggt
    49321 gtccaagcaa cgaattggct tatcaaaaaa ggacacttta gtgctgacag tcgtgttcta
    49381 gaagtagctt gtaatcaatg taccagttcc atcgatatcg ctaaagcatt ttcctgtcac
    49441 attacagcgc ttgatctaga tagtaaagta atagaaaagg ccaaagaaaa tgtcaaaaag
    49501 gcccaattag aagaatttat tgaagttatt cagggaaatg cactcagact gccctttcct
    49561 gataatagtt tatcgttatc aacgaagcaa tgttaacgat gcttaacaat tcagctaagg
    49621 agaaagccat aaaagagtac ttacgtgtcc taaaacctgg tggacgttta ctcacacatg
    49681 atgtttctta tcaagatgaa gacactgcca agctgatcga tcaacttaga catactatga
    49741 atgttaacgt tgctcctcta aaaatagatg attggcaaaa tttattctta caaaatggtt
    49801 tttaaagttc agaatatcaa tttggaaaaa taagtcttat gtccccgtta ggaatgatta
    49861 aagatgaagg tttattcaat accattaaaa tcatcagccg gggactgaaa aatgaacgat
    49921 gccctcatcc aaaattaata aatgttttgg aaagaaaact tgaaattatc cttggtgatc
    49981 agaaacatat ccttgaaaaa gatagcctta ttagcctctc tccac
//
LOCUS       DATRMA010000005        45362 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.005, whole genome shotgun sequence.
ACCESSION   DATRMA010000005 DATRMA010000000
VERSION     DATRMA010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 45362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45362
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.005"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            complement(71..1534)
                     /locus_tag="VBC28_000272"
     CDS             complement(71..1534)
                     /locus_tag="VBC28_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6814278.1"
                     /translation="MSEDQKHPFFEPKKETDGLEFKDAKEMTVEEAVRKDSEIKAGIT
                     EEDSILDKYIKQHRDEVASQKFETKSSDFANLDTASLDDFIKKQREELSAMLAAEELS
                     KKLDNSVSQEQDTEANAVSPKEESSQEQENSVTPVPPLNTEAEPTATEPDSTIADSEE
                     YKSSSKKRGGIVGTLIALILLLIVAIFGYNYFKNNNSTNSQTATSQSSSSKATTTSSE
                     EDKKASQNLDNFNKSYANFFVDDKKTQLKNSEFDKLSELEKKVDALKGTKYYGKVKVK
                     FDSLKRQIDAVKAVNDKFKSPAVVDGKKSEKLEVKDGANFDSLDSKTLNTGNASLDSL
                     LHSIVSTGRNQVKQSEEQASSNKVSDTQITEQPNVTNGQSSSSAATINNQAAGTASGN
                     LERNRSRVPYNNAAIADTGNPAWIFNPGVLEKIVATSQARGYFSGNNYILEPVNIING
                     NGYYNMFKLDGTYLFSINAKTGYFVGNAPGRADSLDY"
     gene            complement(1547..2701)
                     /locus_tag="VBC28_000273"
     CDS             complement(1547..2701)
                     /locus_tag="VBC28_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEO6814279.1"
                     /translation="MKESFKLIATAAAGLEAIVGREIRNLGIDCQVENGRVRFHGDIK
                     TIIETNLWLRAADRIKIIVGEFPAPTFEELFQGVYGLDWENYLPLGAKFPIAKAKCVK
                     SKLHNEPSVQAISKKAVAKKLQKVFHRPEGVPLQENGAEFKIEVSILKDKATVMIDTT
                     GSSLFKRGYRAEKGGAPIKENMAAAIIQLSNWFPDKPLIDPTCGSGTFCIEAAMIGMN
                     IAPGFNRDFAFEAWPWVDQSQVQKVRDEAESKANYDIDLDISGFDLDGRMVEIARKNA
                     EEAGLGDVIKLKQMRLQDLKTDKINGVIISNPPYGERLLDDKAVDILYNEMGQTFAPL
                     KTWSKFILTSDEGFEKKYGSQADKKRKLYNGTLKVDLYQYYGERVRRQVK"
     gene            complement(2784..3152)
                     /gene="rnpB"
                     /locus_tag="VBC28_000274"
     ncRNA           complement(2784..3152)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VBC28_000274"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            complement(3171..3503)
                     /gene="gpsB"
                     /locus_tag="VBC28_000275"
     CDS             complement(3171..3503)
                     /gene="gpsB"
                     /locus_tag="VBC28_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HEO6814280.1"
                     /translation="MASIIYSPKDIFEQDFKVSMRGYDKKEVDVFLDDVIKDYENYLE
                     QIEKLQMENRRLQQALDKKESEASNVRNSGTAMYNQKPIAQSATNFDILKRISRLEKE
                     VFGRQIRE"
     gene            complement(3624..4142)
                     /locus_tag="VBC28_000276"
     CDS             complement(3624..4142)
                     /locus_tag="VBC28_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HEO6814281.1"
                     /translation="MKSTILVTGYKNFELGIFQDKDPRITIIKKAIDKDFRRFLENGA
                     DWFIFMGNLGFEYWALEVALDLQKEYDFQIATIFTFENHGQNWNEANKAKLALFKQVD
                     FVKYTFPSYENPGQFKQYNHFLINNTQGAYLFYDSENETNLKFLLEMMEKKEAYDISF
                     LTFDRLNEIYEE"
     gene            4217..4816
                     /gene="recU"
                     /locus_tag="VBC28_000277"
     CDS             4217..4816
                     /gene="recU"
                     /locus_tag="VBC28_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HEO6814282.1"
                     /translation="MVNYPHQLIRKTTVTKSKKKKIDFANRGMSFEAAINATNDYYLS
                     HELAVIHKKPTPVQIVKVDYPKRSRAKIVEAYFRQASTTDYSGVYKGYYIDFEAKETR
                     QKTAMPMKNFHAHQIEHMANVLQQKGICFVLLHFSTLKETYLLPANELISFYQIDKGN
                     KSMPIDYIRKNGFFVKESAFPQVPYLDIIEEKLLGGDYN"
     gene            4803..7049
                     /locus_tag="VBC28_000278"
     CDS             4803..7049
                     /locus_tag="VBC28_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEO6814283.1"
                     /translation="MITIKKESVIKLLKYAFGIIMGFIILAIVIGGLLFAYYVSRSPK
                     LTDQALKSVNSSLVYDGNNKLIADLGSEKRESVSADSIPLNLVNAITSIEDKRFFKHR
                     GVDIYRILGAAWHNLVSSNTQGGSTLDQQLIKLAYFSTNKSDQTLKRKSQEVWLALQM
                     ERKYTKEEILTFYINKVYMGNGNYGMRTTAKSYFGKDLKELSIAQLALLAGIPQAPTQ
                     YDPYKNPESAQTRRNTVLQQMYQDKNISKKEYDQAVATPVTDGLKELKQKSTYPKYMD
                     NYLKQVISEVKQKTGKDIFTAGLKVYTNINTDAQKQLYDIYNSDTYIAYPNNELQIAS
                     TIMDATNGKVIAQLGGRHQNENISFGTNQSVLTDRDWGSTMKPISAYAPAIDSGVYNS
                     TGQSLNDSVYYWPGTSTQLYDWDRQYMGWMSMQTAIQQSRNVPAVRALEAAGLDEAKS
                     FLEKLGIYYPEMNYSNAISSNNSSSDAKYGASSEKMAAAYSAFANGGTYYKPQYVNKI
                     EFSDGTNDTYAASGSRAMKETTAYMMTDMLKTVLTFGTGTKAAIPGVAQAGKTGTSNY
                     TEDELAKIEATTGIYNSAVGTMAPDENFVGYTSKYTMAIWTGYKNRLTPLYGSQLDIA
                     TEVYRAMMSYLTGGYSADWTMPEGLYRSGSYLYINGTTTTGTYSSSVYKNIYQNSGQS
                     SQSSSSTSSEKQKEDKNTANDANSSSPQVETPNNGNATTPNNSNQTVPGTGHGNGNGN
                     NNTVPNGN"
     gene            complement(7095..8429)
                     /gene="pepC"
                     /locus_tag="VBC28_000279"
     CDS             complement(7095..8429)
                     /gene="pepC"
                     /locus_tag="VBC28_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014607929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase C"
                     /protein_id="HEO6814284.1"
                     /translation="MSKLTQTFTDKLFADYQANTKFSAIENAVTHNGLLKSLETRQSE
                     IENDYVFSIDLTKDEVSNQKQSGRCWMFAALNTFRHKLISDFKLENFELSQAHTFFWD
                     KYEKSNWFMEQIIATANQELSSRKVKFLLDVPQQDGGQWDMVVALFEKYGVVPKTVYP
                     ESVSSSASRELNQYLNKLLRQDAQILRELIAQGADGATVQNKKEELLQEIFNFLAMNL
                     GLPPQSFDFAYRDKDNHYQSDKNITPKAFYQKYVNLDLSDYVSIINAPTVDKPYGQSY
                     TVEMLGNVVGGPAVKYLNLDMKRFKELAIAQMKSGETVWFGSDVGQVSNRQKGILATT
                     TYDFNSSMDIKLSQDKAGRLDYSESLMTHAMVLTGVDLDESGQPLKWKVENSWGEKVG
                     KDGYFVASDAWMDEYTYQIVVRKELLTKEELEAYNAEPITLAPWDPMGALAN"
     gene            complement(8542..9363)
                     /gene="nadE"
                     /locus_tag="VBC28_000280"
     CDS             complement(8542..9363)
                     /gene="nadE"
                     /locus_tag="VBC28_000280"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002949400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HEO6814285.1"
                     /translation="MTLQDQIIKELGVKPVINPSQEIRRSVEFLKDYLLKHSFLKTYV
                     LGISGGQDSTLAGRLAQLAVEELRADTGENYQFIAIRLPYGIQADEEDAQKALDFIKP
                     DIALTINIKEAVDGQVRALNAAGVEITDFNKGNIKARQRMISQYAVAGQYAGAVIGTD
                     HAAENITGFFTKFGDGGADLLPLFRLNKSQGKQLLAELGADKALYEKIPTADLEENKP
                     GIADEIALGVTYQEIDAYLEGKVVSDKSRGIIENWWYKGQHKRHLPITIFDDFWK"
     gene            complement(9360..10820)
                     /locus_tag="VBC28_000281"
     CDS             complement(9360..10820)
                     /locus_tag="VBC28_000281"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEO6814286.1"
                     /translation="MYKDDSLTLHTDLYQINMMQVYFNKGIHNKRAVFEAYFRKVPFE
                     NGYAVFAGLERIVRYLENLSFSDSDLSYLEELGYPEEFLDYLKNLKMELTVKSAKEGD
                     LVFANEPLVQIEGPLAQCQLVETAILNIINYQTLVATKAARIRSVIEDEPLLEFGTRR
                     AQEMDAAIWGTRAAIIGGANATSNVRAGKIFNIPVSGTHAHALVQTYGDDYQAFKAYA
                     ETHKDCVFLVDTYDTLRVGVPNAIRVAKEMGEKINFLGVRLDSGDLAYLSKKVRQQLD
                     DAGFPNAKIYASNDLDENTILNLKMQKAKIDVWGVGTKLITAYDQPALGAVYKIVSIE
                     TDAGSMRDTIKLSNNAEKVSTPGKKQVWRITSRAKGKSEGDYITFADTDVTQLDEIEM
                     FHPTYTYINKTVRDFDAVPLLVDIFDKGKLVYQLPSLQEIQEYGRKEFDQLWDEYKRV
                     LNPQDYPVDLARDVWQNKMDLIDRIRKEALAKGEVR"
     gene            complement(10978..11892)
                     /gene="trxB"
                     /locus_tag="VBC28_000282"
     CDS             complement(10978..11892)
                     /gene="trxB"
                     /locus_tag="VBC28_000282"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEO6814287.1"
                     /translation="MYDTLIIGSGPGGMTAALYAARSNLKVGLIEQGAPGGQMNNTAE
                     IENYPGYDHISGPELSMKMYEPLEKFEVEHIYGIVQRVENDGDVKRVITEDESYEAKT
                     VILATGAKNSLLGVPGEEEYTSRGVSYCAVCDGAFFRDQDLLVVGGGDSAVEEAVFLT
                     QFAKSVTIIHRRDQLRAQKVLQDRAFANEKIKFVWDSVVKEIKGNEIKVSGVTVENLK
                     TGEISEMTFGGVFIYVGLKPHSSMVSELGITDETGWVLTDTNMKTSIPGLYAIGDVRQ
                     KDLRQIATAVGEGAIAGQGVYNYITEHF"
     gene            complement(11961..12185)
                     /locus_tag="VBC28_000283"
     CDS             complement(11961..12185)
                     /locus_tag="VBC28_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4059 family protein"
                     /protein_id="HEO6814288.1"
                     /translation="MFLEIAKVYLQGLGLASITVILVGIIWVFWRAKKNIDKTARERQ
                     SVLYDVLLIALMTAPILAFAFVAIILMIKA"
     gene            complement(12307..13050)
                     /locus_tag="VBC28_000284"
     CDS             complement(12307..13050)
                     /locus_tag="VBC28_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEO6814289.1"
                     /translation="MIKLRQLTKSFSGQKVLDKLDLDIEKGQVVALVGASGAGKSTFL
                     RSMNYLEEPDYGTIEIDDFKVDFKSISKDDILTLRRKLAMVFQQFNLFERRTALDNVK
                     EGLKIVKKMSDQEATRIARDELAKVGLADREKYYPRHLSGGQKQRVALARALAMKPDV
                     LLLDEPTSALDPELVGEVEKSIADAAKQGQTMVLVSHDMNFVYQVADKVLFLEKGRIL
                     ESGTPEQLFNHPLEERTKEFFASYNKSYL"
     gene            complement(13050..13853)
                     /locus_tag="VBC28_000285"
     CDS             complement(13050..13853)
                     /locus_tag="VBC28_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEO6814290.1"
                     /translation="MNQFILTGGWSWYNNLVSQVPAGKLFSWKAVLDAIPSILERLPI
                     TLLLTVAGALFGLILALIFAVVKINRVKILYPIQALFVSFLRGTPILVQLMLSYYGIP
                     LFLKFLNQKYGFDWNINAIPASVFAITAFAFNEAAYTSETIRAAILSVDQGEIEAARS
                     LGMTSAQVYRRVIIPNAAVVATPTLINTLIGLTKGTSLAFNAGIVEMFAQAQIMGGSD
                     YRYFERYISVALVYWAVSFLIEQLGNAIERKMAIKAPRHLTDEIPGGVR"
     gene            complement(13948..14760)
                     /locus_tag="VBC28_000286"
     CDS             complement(13948..14760)
                     /locus_tag="VBC28_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEO6814291.1"
                     /translation="MFKKIILSIATIAATASLAVSVQASEKVELKVATDSDTAPFTYQ
                     KDGKFKGYDVDVVKAVFKGSKYKVTFKTVPFDTISTGIDAGKFDLSANDFSYNKERAE
                     KYLFSDPISRSNYAVVGKKGSHYKSLSDLSGKSTEVLSGVNYAQVLENWNKNHPNKKP
                     IKIKYVSGTTGVTSRLKNIESGKIDFILYDAISSDYIVKDQSLNLSVSPLKGKIGNNK
                     DGLEYLLLPKDKKGKTLQKFINKRIKVLKENGTLARLSKQYFGGDYVSNIDK"
     gene            complement(14898..16241)
                     /locus_tag="VBC28_000287"
     CDS             complement(14898..16241)
                     /locus_tag="VBC28_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008535518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEO6814292.1"
                     /translation="MSFKDFNFKPYIQRALDELKFVDPTDVQAKLIPVVRSGRDLVGE
                     SKTGSGKTHTFLLPIFEKLDESSDDVQVVITAPSRELGTQIYQATKQIAEHSEQEIRV
                     VNYVGGTDKLRQIEKLKVSQPHIVIGTPGRIYDLVKSGDLAIHKAHTFVVDEADMTLD
                     MGFLDTVDKIAGSLPKDVQILVFSATIPQKLQPFLKKYLTNPVMEKIKTATVIADTID
                     NWLLSTKGRDKNAQILELSKLMQPYLAMIFVNTKERADELHSYLSSNGLKVAKIHGGI
                     APRERKRIMNQVKNLEFEYIVATDLAARGIDIEGVSHVINDAIPQDLSFFVHRVGRTG
                     RNGLSGTAITLYQPSDDSDIRELEKLGINFIPKVIKNGEFQDTYDRDRRNNREKSYQK
                     LDTEMIGLVKKKKKKIKPGYKKKIQWKVDEKRRKERRASNRAKGRAERKAKKQSF"
     gene            complement(16339..17349)
                     /locus_tag="VBC28_000288"
     CDS             complement(16339..17349)
                     /locus_tag="VBC28_000288"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEO6814293.1"
                     /translation="MFLSIMAGVIAFVLTVIAIPRFIKFYQLKKIGGQQMHEDVKQHL
                     AKAGTPTMGGTVFLIVALLVSLIFSIILSKENSGNLGATFGILSVVLIYGIIGFLDDF
                     LKIFKQINEGLTPKQKMSLQLIAGLIFYFVHVLPSGTSAINIFGFYLEVGYLYAFFVL
                     FWVVGFSNAVNLTDGIDGLASISVVISLITYGIIAYNQTQFDILLIIVIMIGALLGFF
                     VFNHKPAKVFMGDVGSLALGAMLAAISIALRQEWTLLFIGFVYVFETSSVMLQVAYFK
                     YTKKKTGVGKRIFRMTPFHHHLELGGVSGKGNKWSEWKVDAFLWAIGIFMSAITLAIL
                     YL"
     gene            complement(17351..19609)
                     /locus_tag="VBC28_000289"
     CDS             complement(17351..19609)
                     /locus_tag="VBC28_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEO6814294.1"
                     /translation="MTFFKKLKKIFLDYVIHIRDRRSPQKNRERVGQNLMILTIFLFF
                     IFIINFVIIVGTDSKFGVNLSKEAKKVYQQSMTVQAKRGTIYDRNGNPIAEDATTYSL
                     YAIISKNYTTATGQKLYVQPSQYEKVASILENKLGMKKNLVLKQLNQKKLFQVSFGSS
                     GSGLSYTKMADIKKTMEKSDIKGIGFSTSPGRIYPNGIFASQFIGFTLPQDDGDGKKL
                     VGNTGLEAALNKVLSGTDGKVTYEKDRSGNVLLGTATTERRAVNGKDIYTTLSEPIQT
                     VLETQMDVFAEKTKGKFASATVVNAKTGEILATSQRPTYNPSTLKGYDKKNLGTYNTL
                     LYDNFFEPGSTMKVMTLASAIDSKHFNSTEVYNSAQYKIADAIIRDWDVNEGLSSGSY
                     MTFPQGFAHSSNVGMVTLEQKMGRDKWLNYLSKFKFGYPTRFGMLHESGGLFPSDNEV
                     TIAMSSFGQGIGVTQVQMLRAFTSISNDGVMLQPQFISSIYDPNTGTSRTARKEVVGK
                     PVSKEAASKTRDYMVTVGTDPYYGTLYAAGAPVIQVGNQSVAVKSGTAQIAQEGGGGY
                     LQGKNDTINSVVAMVPSENPDFIMYVTIQQPEKFSITFWKDVVNPVLEQATAMKETIL
                     KPGLNDSEHQTKYKLSKIVGENPGHVAEELRRNLVQPIILGNGSKVSKVSKRPGANLA
                     ENEQLLVLTNKLTELPDMYGWSKANVEQFAKWTGIKVTYKGSTSGKVRKQSIDVGKSI
                     NKIKKIKITIGD"
     gene            complement(19613..19939)
                     /gene="ftsL"
                     /locus_tag="VBC28_000290"
     CDS             complement(19613..19939)
                     /gene="ftsL"
                     /locus_tag="VBC28_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000180570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEO6814295.1"
                     /translation="MTNEKRTEAVTQTLQRHIKTFSRIEKAFYGAIVITAIIMAVGII
                     YLQSNSLQVKQEVNQLNSKINDKQTEFDNAKQEVNELSNRDRITKIAKDAGLTIQNDN
                     IYRKVD"
     gene            complement(19954..20901)
                     /gene="rsmH"
                     /locus_tag="VBC28_000291"
     CDS             complement(19954..20901)
                     /gene="rsmH"
                     /locus_tag="VBC28_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEO6814296.1"
                     /translation="MTNDFHHITVLLHETVDMLDIKPDGIYVDATLGGAGHSEYLLSQ
                     LGPDGHLYAFDQDQKAIDNAHIRLKKYVDTGQVTFIKDNFRNLSSNLKALGVSEINGI
                     CYDLGVSSPQLDERERGFSYKQDAPLDMRMNREQSLTAYDVVNTYSYHDLVRIFFKYG
                     EDKFSKQIARKIEQVRAEKTISTTTELAEIIKSSKSAKELKKKGHPAKQIFQAIRIEV
                     NDELGAADESIQQAMDLLAVDGRISVITFHSLEDRLTKQLFKEASTVEVPKGLPFIPD
                     DLQPKMELVNRKPILPSQEELEANNRAHSAKLRVARRIR"
     gene            complement(20984..22237)
                     /locus_tag="VBC28_000292"
     CDS             complement(20984..22237)
                     /locus_tag="VBC28_000292"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEO6814297.1"
                     /translation="MTYIEILGQNAKKASQSVARLSTASKNEILRDLARNIVADTETI
                     LTENARDVVKAKDNGISEIMVDRLRLNKDRIQAIANGIYQVADLADPIGQVVSGYTNL
                     DGLKILKKRVPLGVIAMIFESRPNVSVDAFSLAFKTGNAIILRGGKDAIFSNTALVNC
                     MRQTLQDTGHNPDIVQLVEDTSHVVAEELMQATDYVDVLIPRGGAKLIQTVKEKSKIP
                     VIETGVGNVHIYIDEFADLDMAAKIVINAKTQRPSVCNAAEGLVVHQAIAKGFLSQLE
                     KMLKESNQSVEFRADEEALQLLENAVAASESDYATEFLDYIMSVKVVDSFEQAISWIN
                     KYSSHHSEAIITNNISRAEIFQDMVDAAAVYVNASTRFTDGFVFGLGAEIGISTQKLH
                     ARGPMGLEALTSTKYYINGTGQVRE"
     gene            complement(22247..23050)
                     /gene="proB"
                     /locus_tag="VBC28_000293"
     CDS             complement(22247..23050)
                     /gene="proB"
                     /locus_tag="VBC28_000293"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEO6814298.1"
                     /translation="MKRHFETTRRIVIKVGTSSLVQTSGKINLSKIDHLAFVISSLMN
                     RGMEVILVSSGAMGFGLDILKMDKRPQEISQQQAVSSVGQVAMMSLYSQIFSHYQTHV
                     SQILLTRDVVVFPESLQNVTNSFESLLSMGILPIVNENDAVSVDEMDHKTKFGDNDRL
                     SAVVAKITKADLLIMLSDIDGLFDKNPNIYDDAVLRSHVSEITDDIIKSAGGAGSKFG
                     TGGMLSKIKSAQMVFDNNGQMILMNGANPRDILKVLDGHNIGTYFAQGK"
     gene            23191..24576
                     /locus_tag="VBC28_000294"
     CDS             23191..24576
                     /locus_tag="VBC28_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEO6814299.1"
                     /translation="MSREYITLSKNIIKHLGGQNNINNVYHCQTRLRFSLNDPTKVNL
                     EQLKTLKEVKTVVISGGQHQIVIGTHVAKVFEEINSLIETNSTTKIEQTKKAKAVSRI
                     IDFVSGTFQPILPALSGAGMIKALLALLLVFKILTPSSQTYILLNLFADGVFYFLPIL
                     IAITAAQKLKANPILALGTVVMLLHPNWANLVASGKPVSLFHTIPFTLTNYASSVIPI
                     ILIICVQAYIEKYLKQIIPKSLRLVLVPMLIFLSMGILSFSILGPMGTIAGQYLAVIF
                     TFLSKYASWAPAFLVGAFAPILIMFGVHSGIAALGITQLAKLGVDSIFGPGMLCSNIA
                     QATAGTVVTLITKEKKLKEIAGPAAITAYMGITEPILYGVNLPKRYPLIASLIGGGLG
                     GLYAGIMNAHRFAVGSSGLPGLFLYISHTSTHLFITMLIAVIITVSTTAILTFILAQY
                     YEKQVSIRQRD"
     gene            complement(24784..26388)
                     /locus_tag="VBC28_000295"
     CDS             complement(24784..26388)
                     /locus_tag="VBC28_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6814300.1"
                     /translation="MNWSRIWELVKINILYSNPQTLSALRKKQEKHPKKEFSAYKSMF
                     RNQLFQILLFSIIYVFLFVSLDFKEYPGYFTFYIGIFTLVSIIYSFIAMYSVFYESDD
                     VKQYAYLPIKSEELYVAKIFATFGMSVTFLMPILTLMIVAYWRIIGGPLAVLLAIINF
                     AILFLSVMVISLYINSLIGRAIIRSANRKLISTILISLATFGAIVPLLFVNMTSQKMV
                     QGKLQDIAPIPYVRGYYDIVTAPFSMESLLNYYLPLLIILFLIGAIYKWVMPRYYQEL
                     LYGQVKQRKVHRQIDFSKRESINKTLVKHHLSSLQNATLLTNTFLMPLLYLAMFIVPI
                     LNNGKEIGRFFNENYFGIAFLAGILIGSLCVMPASIVGVGISLEKSNFYFIKSLPISF
                     SYFLKHKFVTLITLQLAVPTFIYFLVGFFLLKLSILVLLSFILGLVFMGLIEGQFIYR
                     RDYKHLFLNWQEVTQLFNRGLGQWLLVGSLFGMMIIGSFLIGISIFWSMVWNTVAVNI
                     IILIIGLLILSICQYLLLKNFWKKLV"
     gene            complement(26392..27126)
                     /locus_tag="VBC28_000296"
     CDS             complement(26392..27126)
                     /locus_tag="VBC28_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6814301.1"
                     /translation="MIKFEHVSKVYGEKEALSDLTLSVKDGEIFGLIGHNGAGKTTTI
                     SILTSIIDATYGQVYIDDLLLTEHRDQIKKKIGYVPDSPDIFLNLTAEEYWYFLAKIY
                     DVAPEDIEARITKLVDIFELEEQRYNPIESFSHGMRQKVIVIGALLPNPDIWILDEPL
                     TGLDPQASFDLKEMMKEHAKNGKTVIFSTHVLAVAEQLCDRIGILKQGELIFVGSLGE
                     LKMKYPDKDLETIYLELAGRQASREG"
     gene            complement(27146..27451)
                     /locus_tag="VBC28_000297"
     CDS             complement(27146..27451)
                     /locus_tag="VBC28_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEO6814302.1"
                     /translation="MKKECRDFYRQIQHTYNDISVREDAVLSSILLSASNGLIKTSDV
                     PRVAYELTQQLENNEIEKSFESLATVKELKKSAKKLSERYEQNKVTTGLLSKVFGNH"
     gene            complement(27664..29649)
                     /gene="tkt"
                     /locus_tag="VBC28_000298"
     CDS             complement(27664..29649)
                     /gene="tkt"
                     /locus_tag="VBC28_000298"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEO6814303.1"
                     /translation="MTFDTIDQLAVNTVRTLSIDAIQAANSGHPGLPMGAAPMAYVLW
                     NKFLNVNPKTSRNWTNRDRFVLSAGHGSALLYSLLHLAGYDLSIDDLKQFRQWGSKTP
                     GHPEVNHTDGVEATTGPLGQGIANAVGMAMAEAHLAAKFNKPGFDLVDHYTYTLHGDG
                     CLMEGVSQEAASLAGHLKLGKLVLLYDSNDISLDGPTSQSFTEDVKGRFESYGWQHIL
                     VKDGNDLEAIAAAIEAAKAETDKPTIIEVKTIIGFGAEKQGTSSVHGAPLGAEGITFA
                     KKAYGWEYPDFTVPAEVADRFASDLQARGAKAEEAWNDLFAKYEVEYPELATEYKEAF
                     AGQAETVELKAHDLGSSVASRVSSQQAIQQLSTQLPNLWGGSADLSASNNTMVAAETD
                     FQASNYAGRNIWFGVREFAMAAAMNGIALHGGTRVYGGTFFVFSNYLLPAVRMAALQN
                     LPTVYVMTHDSIAVGEDGPTHEPIEQLASVRSMPNLNVIRPADGNETNAAWQRAVSET
                     DRPTMLVLTRQNLPVLEGTSELAQEGVNKGAYILSEAKGELDGIIIATGSEVKLALDT
                     QDKLESEGIHVRVVSMPAQNIFDEQEASYQEQVLPSAVTKRLAIEAGSSFGWGKYVGL
                     NGLTLTIDTWGASAPGNRIFEEYGFTVENAVSLYKEL"
     gene            complement(29774..31204)
                     /locus_tag="VBC28_000299"
     CDS             complement(29774..31204)
                     /locus_tag="VBC28_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001217288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO6814304.1"
                     /translation="MRIEALMEKERRVQYRLLSFLRGSPQAIALKLALLETGLSRATF
                     LKYINNLNSYFEQEKVNCRIVYYKDKLFLEEDYNLSNQEVLKALMKDSIKYTILISLF
                     NQRQFTIVGLSQELMVSEATLNRHLAHLNELLAEFDIAISQGKQIGDELQWRYFYYEL
                     FKQLWSYDKCQNMIKKLDLDSLILLIERLAQHTLTREAHQNLGLWFSICHHRLLAMEK
                     ISDNLKPIVKHYQCNAFYKRLDAALVLYMSRFALEYREGEVLATFAFLHSQNILPINT
                     MEYIMGFGGPIIDCVTETIIYFKKESILADETSDQVIYQLGQLYSHYYFFKGHILVEQ
                     PDLEQTYRLIDHNMRDKLHHISKKIIANVNRIRPLTEDGCSLLTLHLLELLIFSKNSQ
                     KMPFRIGLDMTGNAVEQSLLEYRIRQHFSGNNSIQVEPYDEGKGFDMVIYQSHSRPYK
                     AKLTYCLNKGASERELQEIDSLIYDN"
     gene            complement(31195..32532)
                     /locus_tag="VBC28_000300"
     CDS             complement(31195..32532)
                     /locus_tag="VBC28_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEO6814305.1"
                     /translation="MTEKYVNKVIVILGASFAGMTCAQKLRQLNPNWDIVLIDKEIHP
                     DYVPNGLNWYYRHEISGLNQAMWQTEEEQRLQNIRCLFGLKVEKINKEDRELMLSDGS
                     SVYYDQLICAMGSQAESTYIDGADAQGVLTTKTYATSQNAKQVLDKSHKVAVVGAGII
                     GLDIAYSLHESGKAVTLLEAQERPDFRHTDPDMSLPLLDAMAESKLHFFQNQKVEKIT
                     VTREEKLCLRTLTGDTFTVDAVILAVNFRPDSRLLTGLVDLSVDNSVVVNDYFQTSDP
                     NIYAIGDLIWSYFKGLNSAYYMPLINQAIRSAQMLAYHLSGHAVPKLKITRATGSKHF
                     GYYRANIGLTELEAGFYEDTVSVTYFPKEQYDLRIKLIANQKTGHLLGAQLISKENCL
                     ATANQLVQAISCDMTDFDLAFQDFIYTARESEMAYMLHQAAINLYEKRIGLCE"
     gene            complement(32620..33318)
                     /locus_tag="VBC28_000301"
     CDS             complement(32620..33318)
                     /locus_tag="VBC28_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000350768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEO6814306.1"
                     /translation="MDIFGEFLGTALLVLLGNGVVAGVVLPKTKNHNSGWIVITFGWG
                     LAVAIAALVSGNISPAHLNPAVSLAFAIKGDLAWGTAILYMIAQIIGAMLGSLLVYLQ
                     FRPHYEAAENRADILGTFATGPALKDNFSNFLSEVLGTLVLVLTIFAIGKYNMPPGVG
                     TMSVGMLVVGIGLSLGGTTGYAINPARDFGPRLLHALLPMKNKGDSDWTYSWIPIVGP
                     MVGAILAALIFAMM"
     gene            complement(33330..35159)
                     /gene="glpO"
                     /locus_tag="VBC28_000302"
     CDS             complement(33330..35159)
                     /gene="glpO"
                     /locus_tag="VBC28_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HEO6814307.1"
                     /translation="MEFSRETRRLALQRMQDRTLDLLIIGGGITGAGVALQAAASGLD
                     TGLIEMQDFAEGTSSRSTKLVHGGLRYLKQFDVEVVSDTVSERAVVQQIAPHIPKPDP
                     MLLPVYDEPGSTFSMFRLKVAMDLYDLLAGVTNTPAANKVLSAEDVLKREPDLQKEGL
                     LGGGVYLDFRNNDARLVIENIKRANRDGAYIASHVKAEDFLFDDNNQIIGVRARDLLT
                     DQVIDIKARLVINTTGPWSDTVRNFSNEGKQIHQLRPTKGVHLVVDRQKLNISQPVYV
                     DTGLNDGRMIFVLPREDKTYFGTTDTDYHGDLEHPTVTKEDVDYLLNIVNKRFPEAEL
                     TIDDIESSWAGLRPLLSGNSASDYNGGNSGKLSDESFEELIDSVKDYIAHKNHREDVE
                     KAISHVESSTSEKELDPSAVSRGSSFERDDNGLLTLAGGKITDYRKMAEGAMETIINI
                     LDKEYNRKFKLINSKTYPVSGGEINPSNVDSEIEAYAQLGTLSGLSIEDARYIANLYG
                     SNAPKLFALTRQITEAEGLSLVETLSLHYAMDYEMALSPTDFFLRRTNHMLFMRDNLD
                     SLIQPVIDEMAKHYQWSDQDKTFYEEELHETLKDNDLAALKDN"
     gene            complement(35172..36680)
                     /gene="glpK"
                     /locus_tag="VBC28_000303"
     CDS             complement(35172..36680)
                     /gene="glpK"
                     /locus_tag="VBC28_000303"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000093523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEO6814308.1"
                     /translation="MSSEEKYIMAIDQGTTSSRAIIFNKKGEKIASSQKEFPQIFPQA
                     GWVEHNANQIWNSVQSVIAGAFIESSIKPGQIEAIGITNQRETTVVWDKKTGLPIYNA
                     IVWQSRQTAPIADQLKQEGHTNMIHEKTGLVIDAYFSATKVRWILDHVPGAQERAEKG
                     ELLFGTIDTWLVWKLTDGLIHVTDYSNAARTMLYNIKELKWDDEILELLNIPKAMLPE
                     VKSNSEVYGKTTPFHFYGGEVPISGMAGDQQAALFGQLAFEPGMVKNTYGTGSFIIMN
                     TGEEMQLSQNNLLTTIGYGINGKVHYALEGSIFIAGSAIQWLRDGLRMIETSSESEGL
                     AQSSTSDDEVYVVPAFTGLGAPYWDSNARGSVFGLTRGTSKEDFVKATLQSIAYQVRD
                     VIDTMQVDSGIDIQQLRVDGGAAMNNLLMQFQADILGIDIARAKNLETTALGAAFLAG
                     LSVGYWESMDELKELNATGQLFQATMNESRKEKLYKGWRKAVKATQVFAQED"
     gene            complement(36795..37058)
                     /locus_tag="VBC28_000304"
     CDS             complement(36795..37058)
                     /locus_tag="VBC28_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HEO6814309.1"
                     /translation="MIELLLALLLWNILVFATYAIDKRRAIRGAWRISEKTLMIESLL
                     LGGFGAFLGGEVFRHKTTKWYFRLVWYIGMIIDLAIIWLIWCY"
     gene            complement(37147..37404)
                     /locus_tag="VBC28_000305"
     CDS             complement(37147..37404)
                     /locus_tag="VBC28_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HEO6814310.1"
                     /translation="MDPKKIARINELSKKKKTVGLTGEEKVEQAKLREEYIEGFRRSV
                     RHHVEGIKLVDDEGNDVTPEKLRQVQREKGLHGRSLDDPNS"
     gene            complement(37416..39455)
                     /locus_tag="VBC28_000306"
     CDS             complement(37416..39455)
                     /locus_tag="VBC28_000306"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEO6814311.1"
                     /translation="MTKDLLLELGLEELPAYVVTPSEKQLGQKMVKFLEDHRLSFETV
                     QIFSTPRRLAVRVKGLADQQTDLTEDFKGPSKKIALDAEGNFSKAAQGFVRGKGLSVD
                     DIEFREVKGEEYVYVTKHETGKSAIDVLASVTEVLTELTFPVNMHWANNSFEYIRPVH
                     TLVVLLDDQALELDFLDIHSGRISRGHRFLGSDTEISSASSYEDDLRQQFVIADAKER
                     QQMIVNQIHAIEEKKNISVEIDEDLLNEVLNLVEYPTAFLGSFDEKYLDVPEEVLVTS
                     MKNHQRYFVVRDRDGKLLPNFISVRNGNAEHIENVIKGNEKVLVARLEDGEFFWQEDQ
                     KLNIADLVEKLKQVTFHEKIGSLYEHMDRVKVISQYLAEKADLSDEEKLAVLRAASIY
                     KFDLLTGMVDEFDELQGIMGEKYALLAGEQPAVAAAIREHYMPTSADGELPETRVGAI
                     LALADKFDTLLSFFSVGLIPSGSNDPYALRRATQGIVRILEAFGWDIPLDELVTNLYG
                     LSFASLDYANQKEVMAFISARIEKMIGSKVPKDIREAVLESDTYIVSLILEASQALVQ
                     KSKDAQYKVSVESLSRAFNLAEKVTHSVLVDSSLFENNQEKALYQAILSLELTEDMHD
                     NLDKLFALSPIINDFFDNTMVMTDDEKMKQNRLALLNSLVAKARTVAAFNLLNTK"
     gene            complement(39459..40100)
                     /locus_tag="VBC28_000307"
     CDS             complement(39459..40100)
                     /locus_tag="VBC28_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001094117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEO6814312.1"
                     /translation="MNTLIVNSHPDFSNPYSFTTILQEKFIELYNEHFPNHQLSILNL
                     YDCVLPEITKEVLLSIWSKQRKGLELTADEIVQAKISKDLLEQFKSHHRIVFVSPMHN
                     YNVTARAKTYIDNIFIAGETFKYTENGSVGLMTDDYRLLMLESAGSIYSKGQYSPYEF
                     PVHYLKAIFKDFMAFDDFCVVRAEGTDILDRQVVLDKMNQDLREAFEAFYSKE"
     gene            complement(40100..41014)
                     /gene="glyQ"
                     /locus_tag="VBC28_000308"
     CDS             complement(40100..41014)
                     /gene="glyQ"
                     /locus_tag="VBC28_000308"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEO6814313.1"
                     /translation="MSKKLTFQEIILTLQQFWNDQGCMLMQAYDNEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPSNIQELYLKSLEL
                     LGINPLEHDIRFVEDNWENPSTGSAGLGWEVWLDGMEITQFTYFQQVGGLQTGPVTSE
                     VTYGLERLASYIQEVDSVYDIEWAPGVKYGEIFTQPEYEHSKYSFEISDQVMLLENFE
                     KFEREAKRALEEGLVHPAYDYVLKCSHTFNLLDARGAVSVTERAGYIARIRNLARVVA
                     KTFVAERKKLGFPLLDEETRIKLLAEED"
     gene            complement(41327..41869)
                     /locus_tag="VBC28_000309"
     CDS             complement(41327..41869)
                     /locus_tag="VBC28_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HEO6814314.1"
                     /translation="MSIIIGLMAAMFIIRLAYLKLSIANEKALRKNGAKEYGVGVSKA
                     ITVLHIIIYFSSVTEAILTKASFNFVSVIGLSLMIFSVFMLHTVTRLLGRIWTVKLMV
                     DKNHQFVDHWLFRVVKHPNYFLNIAPELLGVTLLCHAKYTALFVLPIYAFVIYLRIRE
                     ENLLLKTIIIPNGIKKSRVY"
     gene            complement(41988..43136)
                     /gene="nagA"
                     /locus_tag="VBC28_000310"
     CDS             complement(41988..43136)
                     /gene="nagA"
                     /locus_tag="VBC28_000310"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEO6814315.1"
                     /translation="MTKYIKADRFFYADHVKENGYLEIKDNHFGKWIENISGQEEILD
                     YSGYQIAPGLVDTHIHGFAGADVMDCDSEGILRMSAGLLSTGVTSFLPTTLTSDTKRL
                     EEASKSVAAVAGKEQGAKIQGIYFEGPYFTEEYKGAQNPIYMRNPNLEEFAQWQKAAK
                     GLITKIALAPEREGVEEFVSAITKQGVTVALGHSNGTYKEAKKAVKAGASVWVHAYNG
                     MRGLTHREPGMVGAVYNLPNTYAELICDGHHVDPVACDILMTQKGHNHVALITDCMAA
                     GGAPDGDYMLGELPVVVSNGTARLKDGGNLAGSILKLKDGLKNVVEWGIATPEEAVRM
                     ASYVPAVSVGIDNVCGQIKEGYAADFIVLDKHLDLVATYLDGEKRWQS"
     gene            complement(43414..44121)
                     /locus_tag="VBC28_000311"
     CDS             complement(43414..44121)
                     /locus_tag="VBC28_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001871310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HEO6814316.1"
                     /translation="MKIDKKELLALIASIILLIFASVTFFLFKDHGTTQMDTVESSVN
                     HVSDSQLTEAQDMLDKFEKKPSEKLLKDVELALNKLSNSSKKEALQKRFKKAKDKYLK
                     DEADKKATKDATDLVEILEQAPSEENVLKAEAAVNKLTVKESKEALQKRIDTVKTQYG
                     LIGNQTPSSSVAETTEQGTANPASQDTSSYVNQNVAPTYEQPQANNTPVTPGVNNTVP
                     TPGTGTVPATNGTGVAQ"
     gene            44313..44624
                     /locus_tag="VBC28_000312"
     CDS             44313..44624
                     /locus_tag="VBC28_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEO6814317.1"
                     /translation="MIPSQMLKGLLEGAILKIIQQEETYAYEISKKLNSYGFGEISEG
                     TIYPIILRLQSNKYIKGQQKPSPQGPKRKYYTLTNEGVEQLAQFKQNWESLSKALNKL
                     L"
     gene            44645..45328
                     /locus_tag="VBC28_000313"
     CDS             44645..45328
                     /locus_tag="VBC28_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6814318.1"
                     /translation="MDKLKKLQKENTLLEGRIDNSNNQTYTDMIVYLRGASISPYHQE
                     LIRNDIVNMLLEAQERQASLVSVFGEDRHDFINQVIKSTPKISKKEETLQRWDLAILL
                     LTIQMIIFLGGYLITEALQQSVPDLIPITLLDVLFAIFISIIAVKIADTIIYATYNFD
                     KSKEKKYFFRYIFLILSLIIAYILIGKYYHLPFINIPLWIYLIILGLSFSLHIIVKKY
                     LNKHYYIEF"
BASE COUNT        14350 a         9202 c         7092 g        14718 t
ORIGIN      
        1 aacaccctaa tcgttagact gattttctaa cttttagggt gtagacaaaa aactggtttt
       61 gttctttata ttaataatct aaactatcag ctcttccagg agcgttacct acaaagtaac
      121 ctgtcttggc attgatagag aacaaataag tgccatctaa tttaaacata ttgtaataac
      181 cattaccatt gataatattt acaggttcaa gaatatagtt atttccagag aaatagcctc
      241 gcgcctgtga agtggccacg attttctcta atactcctgg gttaaatatc caggcaggat
      301 ttcctgtatc cgcaatagca gcattattat acggtactcg actacggtta cgttccaaat
      361 tacctgatgc agtgccagct gcctgattat ttattgtagc agctgatgag gaagattgac
      421 cgttagttac gtttggttgt tcagttattt gagtgtctga aaccttatta gacgaagcct
      481 gttcttcgga ttgttttacc tgattacgac cagttgaaac gatggagtga agtaaactat
      541 ctagactagc attaccagta ttaagagttt tactatctaa agagtcaaaa ttcgctccat
      601 cttttacctc taatttttca ctcttcttac catcaactac agcaggcgac ttaaacttgt
      661 cgttaacagc tttaacagca tcaatttgac gttttagact atcaaattta acttttactt
      721 taccgtagta tttagttcct tttaaagcat caactttctt ttctaattct gacaatttat
      781 caaattcgct atttttgagt tgggttttct tatcatcaac aaaaaagtta gcataagatt
      841 tgttgaaatt atctaagttc tgactagctt ttttatcttc ttctgatgaa gtcgtcgtag
      901 ccttagaact agaactttga cttgttgcag tttgagaatt tgtcgaatta ttattcttga
      961 aatagttgta accaaagatt gctactatta gaagtaaaat aagagcaatc aatgttccta
     1021 cgatacctcc acgcttctta gaagaggatt tatactcctc tgaatcagca atagtcgaat
     1081 ctggttctgt tgcagttggt tctgcttccg tattcagagg aggaactggg gttacactat
     1141 tttcttgttc ttgtgaagat tcttctttag gtgagacagc attagcttct gtatcttgtt
     1201 cttgagagac tgaattatcc aactttttag aaagctcttc tgcagctaac atcgctgata
     1261 attcttcgcg ctgtttcttg ataaaatcat ctaaagatgc cgtatcaagg tttgcaaaat
     1321 cgcttgattt tgtctcaaac ttttgtgaag ctacttcatc acgatgttgt ttgatgtatt
     1381 tatcaagaat actatcttct tcggtaatac cagccttaat ttcagaatct tttctaacag
     1441 cctcttctac tgtcatttct ttagcatctt taaattcaag accatcagtt tctttttttg
     1501 gttcaaaaaa tgggtgtttt tgatcttctg acatgagttc tccccgttat tttacttgac
     1561 gtctcacacg ttcaccgtaa tattggtaca aatcaacttt taaagtacca ttgtaaagct
     1621 tacgtttttt atcagcttgt gagccgtatt ttttttcaaa tccttcgtca ctagttagga
     1681 taaacttgct ccaagttttt aaaggcgcaa atgtttgtcc catctcatta tacaaaatgt
     1741 ccactgcttt gtcatccagg agtcgttcac catatggtgg gttggagata ataacaccgt
     1801 taattttatc tgtttttaaa tcttgaagac gcatttgttt caatttgata acatctccaa
     1861 gtcctgcttc ttctgcattt tttctagcaa tttctaccat acgaccgtct aaatcaaaac
     1921 ctgaaatatc taaatcaata tcatagtttg ctttactctc agcttcatca cgaacctttt
     1981 gtacttgact ttgatctacc catggccacg cctcaaaggc aaaatcacga ttaaaaccag
     2041 gagctatatt cattccaatc atagcagctt caatacaaaa agtacctgaa ccacaagttg
     2101 gatcaatcaa tggcttatca gggaaccaat tagataattg aataatagct gctgccatgt
     2161 tttctttaat tggtgcacca cctttttctg cacggtagcc acgtttgaag agacttgatc
     2221 cagtcgtatc aatcatgaca gtcgctttat cttttaaaat agaaacttct attttaaatt
     2281 cagctccatt ttcttgtaag ggaactcctt caggacggtg aaagactttt tgaagtttct
     2341 tagcaacagc ttttttagaa atggcttgta cactaggttc attgtgcaat tttgacttga
     2401 cacactttgc tttagcaata ggaaattttg cccctaacgg taaataattt tcccagtcaa
     2461 ggccatatac accctgaaaa agttcctcaa aagtaggtgc tggaaactct cctacaataa
     2521 tctttatacg atcggcagct cgtagccata ggttcgtttc aatgattgtt ttgatatctc
     2581 cgtggaaacg gactctaccg ttctcaactt ggcagtcaat tcctaggttt ctgatttctc
     2641 taccaacaat ggcttctaaa ccagcagcag cagttgctat taatttgaat gattctttca
     2701 tttttcctct tttacactat cgtattcatc acagttaaaa attggctgag atgttatctc
     2761 agccactaac ctattatgca attttctata agccatgttt tgttccagaa acaactagga
     2821 attgtttctt tcgataatca tctgtctact gcaaagcagt caggatagat tgttctcatt
     2881 ccaactatcc catgccccta ccaaagtttg ggttgctagc ttgaggggtt taccgcgttc
     2941 cactttctag atttccctag aaactacgtc actgtggcac tttcaaagct attcaggcat
     3001 atccaaagac ttagcccttt tgcttgccgt aagatagcta gctatctccc taggcttatt
     3061 ttttcgccta gcacaaacac tactggcatc acagccagtg ctagcatgga ctttcctcat
     3121 agtatctcaa tactacgcga ttatccaaaa attgcatatt tgagaaatcc ttattctcta
     3181 atttgtcttc caaaaacttc tttctcaagg cggctaatgc gttttaaaat atcaaaattg
     3241 gttgcagatt gggcaatcgg tttttgatta tacatagctg tccctgaatt tcttacattt
     3301 gaagcttcgc tttctttttt gtctaaagct tgttgtagac gtctattttc catttggagc
     3361 ttctcaattt gctcaagata attttcgtaa tctttgatga catcatccag aaatacatcg
     3421 acttctttct tgtcatatcc ccgcatacta actttaaaat cttgctcaaa aatatctttt
     3481 gggctataaa taatacttgc cattctattc tccaaaccag tcaaatcgtt ttaatataag
     3541 tcctatcctt tatatcaaag taaataatac ctaaattaaa tggaaaaatc aagcgtaaaa
     3601 cgttctcata tccgaaaaaa cgatcattcc tcatatattt catttaagcg atcaaaagtt
     3661 aggaaagaaa tatcataagc ttcctttttc tccatcattt ctagcaaaaa ttttaaatta
     3721 gtttcatttt cagaatcata aaaaagatat gcaccttgtg tgttgttaat gagaaaatga
     3781 ttgtattgtt taaattgacc cgggttttca tagcttggga aagtatattt aacaaagtca
     3841 acttgcttaa agagtgccaa cttagcttta ttagcctcat tccaattttg gccatggttc
     3901 tcaaaggtaa aaattgtcgc aatttgaaaa tcatattctt tttgtaaatc aagagcaact
     3961 tccaaagccc agtactcaaa ccctaaattt cccataaaaa taaaccagtc tgctccattt
     4021 tccaagaagc gtctaaaatc cttatcaata gcctttttta taatagtaat ccgaggatcc
     4081 ttatcctgga aaatccctaa ttcaaaattt ttgtaacctg ttacaagaat agttgacttc
     4141 atttgttgtc catcctaatt ttatggtata atagttttgc ttatcttagc attttatttg
     4201 taatgcaagg agaaaaatgg ttaactatcc acatcagctt attcggaaaa caacagttac
     4261 aaaatcaaag aaaaagaaaa tcgattttgc caaccgtggt atgtcatttg aagctgctat
     4321 caatgcaacc aatgactact atttgtctca cgaattagct gtcattcata agaaaccaac
     4381 gccagtacaa atcgtcaagg tagattatcc taaacgtagt cgtgctaaga ttgtagaagc
     4441 ttattttaga caagcttcta ctactgatta ttctggtgtt tacaaaggtt actatattga
     4501 ctttgaagcc aaagaaaccc ggcagaaaac tgctatgcct atgaaaaatt ttcatgctca
     4561 ccaaatagag cacatggcaa atgtattaca gcaaaaaggg atttgctttg tcttgcttca
     4621 tttttccaca cttaaggaaa cctatctact ccctgctaat gagttaattt cattttatca
     4681 gattgataaa ggcaataaat caatgcctat tgattatatc agaaaaaatg gatttttcgt
     4741 aaaggagagt gcctttcctc aagtccctta cttagatatt attgaagaaa aattattagg
     4801 cggtgattac aattaaaaag gaatctgtta tcaaactatt gaagtatgct tttggcatta
     4861 taatgggatt tattatctta gctattgtaa taggtgggct cctatttgca tactacgtta
     4921 gtcgttctcc gaaattaacc gatcaagctt taaaatccgt taactctagt ttggtttatg
     4981 atggtaataa taaacttatt gccgatttag gctcagaaaa gcgtgaaagt gttagtgcgg
     5041 atagcattcc actaaatttg gttaacgcta tcacttctat cgaagataaa cgtttcttta
     5101 aacatagagg tgtcgatatt tatcgtattt taggtgcagc ttggcataat cttgttagta
     5161 gtaatacgca aggtggttca acccttgatc aacagttgat taaactggct tacttttcta
     5221 ccaataaatc tgaccaaacg ttaaaacgta aatcacagga agtttggctt gcgcttcaaa
     5281 tggagcgtaa atacaccaaa gaagaaattc ttactttcta tattaataaa gtttatatgg
     5341 gaaatgggaa ttatggtatg agaacaacag ctaaatcata ctttggtaaa gacctaaagg
     5401 aattatctat tgcacaactt gctttgctcg ctggtattcc tcaagcacct acacaatatg
     5461 acccttataa aaacccagaa tctgctcaaa caagacgtaa taccgttctt cagcagatgt
     5521 atcaagataa aaacatttct aaaaaggaat acgaccaagc tgttgcaact ccagtaactg
     5581 atggcttaaa agaattaaag caaaaatcta cttatccaaa atatatggat aactacttaa
     5641 aacaagttat tagtgaagtt aaacaaaaaa ctggtaaaga tatctttact gctgggctaa
     5701 aagtgtatac taatatcaac actgatgcac aaaaacaact atatgacatc tacaacagtg
     5761 acacttacat cgcttatcca aacaatgaat tacaaatagc atctaccatc atggatgcga
     5821 ctaatggtaa agtcattgca caattaggcg ggcgtcatca gaatgaaaat atttcatttg
     5881 ggacaaatca atctgtctta acagaccgcg attggggttc tacaatgaaa cctatctcag
     5941 cttatgcacc tgctattgat agtggtgtct ataattcaac aggtcaatca ttaaacgact
     6001 cagtttacta ctggcctggt acttctactc aactatatga ctgggatcgt caatatatgg
     6061 gttggatgag tatgcagacc gctattcaac aatcacgtaa cgtccctgct gtcagagcac
     6121 ttgaagccgc tggattagac gaagcaaaat ctttccttga aaaattaggc atatactatc
     6181 cagaaatgaa ctattcaaat gctatttcaa gtaacaacag tagcagtgat gcaaaatatg
     6241 gtgcaagtag tgagaaaatg gcagcggctt actcggcttt tgcaaacggc ggaacttact
     6301 ataaaccgca atatgttaat aaaattgaat ttagcgatgg aaccaatgat acttatgcag
     6361 cgtctggtag ccgtgcgatg aaagagacta ctgcctacat gatgacggat atgctgaaaa
     6421 cagtactaac atttggtact ggtactaaag cagctatccc tggtgttgca caagctggta
     6481 agactggtac ttccaactat acggaagatg agttagctaa aattgaagca actactggta
     6541 tctacaatag cgccgttggt acaatggctc ctgatgaaaa ctttgtcggc tatacttcta
     6601 agtacacaat ggcaatttgg actggttata aaaatcgcct tacaccactt tatggtagcc
     6661 aactggatat tgctactgag gtttatcgtg caatgatgtc ctacctaact ggcggataca
     6721 gtgcagattg gacaatgcca gaagggctct atcgaagcgg aagctacttg tatatcaatg
     6781 ggacaactac aaccggcact tactcttctt ctgtctataa gaatatctat caaaattctg
     6841 gacagtcaag tcaaagcagt agttcaacat cctctgagaa acaaaaagag gataagaata
     6901 cagcaaatga tgccaattca tcatccccac aagtcgaaac acctaataat ggtaatgcta
     6961 ctacccctaa taattccaat caaactgtgc caggaactgg acatggtaat ggtaatggta
     7021 acaacaatac agtacctaac ggtaattaaa aaactggata tattatccag ttttttaatt
     7081 tataattact attactaatt agctaatgct cccatcggat cccacggcgc taaagtaatt
     7141 ggctctgcgt tgtaagcctc taattcttct ttagtaagca attctttacg aacgactatt
     7201 tggtaagtat attcatccat ccaagcatct gaagctacaa aataaccatc cttaccaact
     7261 ttctctcccc aagagttctc aactttccat tttaaaggtt gtccagattc atcaaggtct
     7321 actcccgtta agaccatagc atgtgtcatt aaactttcac tataatctag gcggcccgct
     7381 ttgtcttggc taagtttaat atccatagat gaattaaaat cataagtcgt agttgctagg
     7441 ataccctttt gacgatttga tacttgacct acgtctgaac caaaccacac agtttctcct
     7501 gatttcattt gcgcaatcgc taactcttta aaccgtttca tatcaaggtt taaatattta
     7561 acagctggcc ctccgacaac atttccaagc atctcaactg tataggattg tccataaggc
     7621 ttatctacag ttggagcatt gataatagaa acataatctg ataggtcaag attaacatat
     7681 ttttgataaa atgctttcgg tgtaatattt ttatcagact gataatgatt gtctttatca
     7741 cgataagcaa aatcaaaaga ttgcggtggc aagccaaggt tcattgctaa aaagttaaaa
     7801 atttcttgaa gcaattcttc ctttttattt tgaacagttg ccccatctgc tccctgtgct
     7861 attaattctc gtagaatctg tgcatcttgg cgtaagagtt tattcaaata ttgatttagt
     7921 tcacgactag cacttgatga aacagattca ggataaactg ttttaggaac gacaccatat
     7981 ttctcaaaca gagcaaccac catgtcccat tgtcctccat cttgttgagg tacatctaat
     8041 aggaatttca ccttacgact actcaattcc tgatttgcag tcgcaataat ttgttccata
     8101 aaccaatttg atttttcata cttatcccag aaaaatgtat gtgcctgaga taattcaaaa
     8161 ttctcaagtt taaaatctga aatcagttta tgacgaaatg tattcagtgc agcaaacatc
     8221 cagcaacgtc ctgattgttt ctgattagaa acttcatctt tggtcaaatc aattgaaaag
     8281 acatagtcgt tctcaatctc actttgacga gtctctagag acttcaataa accattgtga
     8341 gttactgcat tttcaatagc gctaaatttt gtatttgctt ggtaatcagc aaataactta
     8401 tctgtaaatg tttgagttaa ttttgacata aattcctcct tttatactta gctactatta
     8461 taaacattct gaatatttct gcaagtctga aagtaattta ttagagaaac aaaaatagct
     8521 agaatattct agctattttt cttatttcca gaaatcatca aaaattgtga ttggaagatg
     8581 acgtttatgt tgacccttat accaccaatt ttcaatgata cctcgagact tgtccgaaac
     8641 caccttccct tcaagataag catcaatttc ttggtaggtt actccaaggg ctatttcatc
     8701 agcgattcct ggtttatttt cttctaaatc tgcggttgga atcttttcgt acaaggcttt
     8761 atcagcacca agttcagcta ataactgttt cccctgactt ttattaagtc taaagagagg
     8821 taataagtca gcacctccat caccaaattt ggtgaaaaaa cctgtaatat tttctgcagc
     8881 atgatcagta ccaataaccg ctccagcata ttgacctgca actgcatatt gactaatcat
     8941 tctttgacga gctttaatat ttcctttatt aaagtctgta atctctactc ctgctgcatt
     9001 aagagctcta acttggccat caacagcttc tttaatatta atggtcaaag caatatctgg
     9061 cttgataaaa tctaatgctt tttgtgcatc ttcttcatcg gcctgaatac catatggtaa
     9121 acgaatagct ataaattgat agttttcccc tgtatcagca cgcaactctt caactgctag
     9181 ttgagctaga cgtcctgcta aagttgaatc ttgcccccct gaaataccca gtacataggt
     9241 ctttagaaaa ctatgtttta atagatagtc ttttaaaaat tctacggaac gacggatttc
     9301 ttgacttgga ttaataacag gttttacacc gagttctttg ataatttgat cttgcaaagt
     9361 catcttactt ctccttttgc caaagcttct ttacggatac gatcaatcag gtccatttta
     9421 ttttgccaaa catcgcgtgc caaatcaact gggtaatcct gtggattcag aacacgttta
     9481 tactcatccc aaagctgatc aaattctttg cgtccgtact cttgaatttc ttgaagactt
     9541 ggtaattgat aaactaattt ccctttgtca aagatatcta ccagtaaggg tactgcgtca
     9601 aaatcacgga ctgttttatt gatataagta taagttggat gaaacatttc aatttcatct
     9661 aattgtgtca catcagtgtc tgcaaatgtg atgtaatcac cctcagattt ccctttagct
     9721 cgactggtga tacgccaaac ttgctttttc ccaggtgttg atactttttc agcattgttt
     9781 gatagtttaa ttgtatctcg catactaccg gcgtcagtct caatagaaac aattttataa
     9841 acagcaccta gcgcaggctg atcataggct gtaattaatt ttgttccgac accccaaaca
     9901 tcaattttag ctttttgcat ttttaggttg aggattgtat tttcatctag atcattcgaa
     9961 gcataaatct ttgcattagg aaaaccggcg tcatctaact gctgacgaac tttcttagac
    10021 aaataagcta aatcacctga atcaaggcga actcccaaga aattaatctt ttcgcccatc
    10081 tcttttgcaa ctcgaatagc attaggcaca cctacacgaa gggtatcata agtatccact
    10141 aaaaagacgc aatctttatg agtttctgcg taagctttaa atgcctggta atcatcgcca
    10201 taagtttgaa ctaaagcatg tgcatgcgtt cctgatactg gaatattaaa gattttacca
    10261 gcacgaacat tgctcgttgc gttagcacca ccaataatcg ctgccctagt accccaaatg
    10321 gctgcatcca tttcttgagc acgtcgtgtc ccaaactcta atagcggttc atcttcaata
    10381 acagaacgaa tacgagctgc ttttgtagca acaagtgtct ggtagttgat gatatttaaa
    10441 atcgctgtct ctactaactg acattgtgct aaaggacctt caatttgaac taatggttca
    10501 ttagcaaata caaggtcacc ttccttggct gatttgacag tcagttccat ttttaaattt
    10561 tttaagtaat ctaaaaattc ttcaggataa cctaactctt ctaaatagga taaatcacta
    10621 tcagaaaagc tcagattttc taaataacga acaatacgct ctaaacctgc aaaaacagca
    10681 taaccatttt caaatggaac cttgcgaaag taagcttcaa acaccgctct tttattgtga
    10741 atacctttat taaaataaac ttgcatcata ttgatttgat ataaatcagt atgtagtgtt
    10801 aaactatcgt ctttatacat cagacaatct ccttttaatc atgagcctag gataaggcta
    10861 tatgtctaac attatagcat atttctatct cggtagttca gtttatcacc ttactttcat
    10921 gaaaataaaa agaaagacta atcgtttttc gatagtcttt tctactttta gtatttctta
    10981 gaaatgctca gttatatagt tatagacacc ctgtccagcg atagcacctt caccaacagc
    11041 agtcgcaatc tgacgtagat ctttttgacg aacatcccca atagcatata gtcctggaat
    11101 tgatgttttc atatttgtat cagttaagac ccagccagtc tcgtccgtaa tacctaattc
    11161 tgagaccatg ctagaatgtg gtttcaaacc aacgtaaatg aaaacacctc cgaaagtcat
    11221 ctctgagatt tctccagtct ttagattttc aacagttaca ccagatactt taatctcatt
    11281 tcctttaatc tctttgacca ctgaatccca tacaaatttt attttttcgt tagcaaaagc
    11341 tcggtcttga agtacttttt gagctctcaa ttgatcacgt cggtgaataa tagtaacact
    11401 tttagcaaat tgagttaaga agacagcttc ttcaacagca gaatcaccgc cacccacaac
    11461 taaaagatcc tgatcacgga aaaatgcacc atcacaaacc gcacagtaag aaacgccacg
    11521 actagtatat tcctcttcac ccggaactcc taaaaggctg tttttagctc cggtagcaag
    11581 gattactgtt ttggcttcat acgattcatc ttcggtgata acacgcttta catcaccatc
    11641 gttctcaact ctttgaacta taccataaat atgctctact tcaaattttt ccaacggctc
    11701 atacatcttc attgataatt ctggacctga gatatgatcg tagcctggat agttctcaat
    11761 ttcagcagta ttattcatct gtccacctgg agcaccttgt tcaattaacc caactttaag
    11821 gtttgaacgt gcagcgtaaa gcgctgctgt cataccacca ggacctgaac ctattattaa
    11881 agtatcatac attactattt ctcctttgaa tcttatggtt ttattctata gcaaagcgtc
    11941 tatatttcca actaatctga ctaagctttt atcattaaaa tgatagctac aaatgcaaag
    12001 gcaagtattg gagctgtcat aagagctatc agaaggacat cataaagaac agattggcgt
    12061 tctctagctg ttttatctat gtttttcttg gcacgccaaa agacccaaat tataccaact
    12121 aaaataacag ttatgctggc aagccctaaa ccttgtaaat aaacttttgc tatttctaaa
    12181 aacatctcta tccaacaagc gattgtcaaa acatatcgca gctttcttat atacttagcc
    12241 taactcaaga ataattaaaa atgaagatta gacattctaa ccttcattat atcaaaatat
    12301 tttagcttat aggtacgatt tgttataact agcgaagaat tcttttgtac gttcttctaa
    12361 aggatggtta aataattgtt caggcgtccc agactctaaa atacgacctt tttccaaaaa
    12421 taaaacttta tctgctactt ggtaaacaaa attcatatca tgactaacaa gtaccatggt
    12481 ttgcccctgc ttagcagcat ctgcaataga tttttcaact tctccaacta attctggatc
    12541 aagtgctgat gtcggttcgt ctaaaagaag aacatcaggt ttcatggcta atgcgcgtgc
    12601 caaagcaaca cgttgtttct gtccacctga taaatgtctt ggataatatt tttcacgatc
    12661 tgcaagtccg acctttgcca gttcgtctct tgcaatccgt gttgcctctt gatctgacat
    12721 ctttttgaca attttcaaac cttcttttac attatccaaa gctgtacgac gctcaaatag
    12781 attgaattgt tgaaaaacca tggctaactt acggcgtaat gttagtatgt catctttact
    12841 gattgattta aaatcaactt taaaatcatc aatttcaatt gttccataat caggctcttc
    12901 taaataattc atactacgaa gaaaggttga ttttcctgca cctgaagcac ctactagagc
    12961 aacaacttga cctttttcaa tatctaaatc aagtttgtct aacacttttt gaccagaaaa
    13021 tgatttagtc aattgtctca attttatcat tagcgtactc ctcctggaat ctcatcagtt
    13081 aaatggcgtg gagccttaat tgccattttt cgttcaatag catttcccaa ttgttcaatt
    13141 aagaaactaa ccgcccagta aacaagtgcc acagagatat aacgttcaaa atagcgataa
    13201 tcgctaccac ccataatttg tgcttgggca aacatttcaa caataccagc attaaaagct
    13261 aaagaggttc ccttagttag accaattaac gtgtttatca aagtaggtgt tgcgactact
    13321 gctgcgtttg gaataataac acgacgataa acttgcgctg aggtcatccc taaactacgt
    13381 gctgcctcaa tttcaccttg atcaaccgac aaaatcgccg ctcgtatagt ttcacttgta
    13441 taagccgctt cattaaaagc aaatgcagta atagcaaata cagaagccgg tatagcatta
    13501 atgttccaat caaaaccata tttttggttt aaaaatttca agaaaagtgg aattccataa
    13561 taactcagca ttaattgaac aagtattgga gtcccacgta aaaaactaac aaataaagct
    13621 tgaatcggat ataaaatttt gacacggtta attttcacta ctgcaaaaat caaagctaaa
    13681 atgagtccaa ataaggctcc tgctactgtc aataatagtg ttatgggtaa tcgctctaaa
    13741 attgagggta ttgcatctaa aacagccttc caactaaata atttccccgc tggaacctga
    13801 ctaaccaaat tattatacca agaccatcca cccgtcaaga taaattgatt cattccttct
    13861 actttccttt tctaaacttt cttatctccg ttattctatc acgcaaactc tcacatgtaa
    13921 aaagataaaa cttatagtct cactgattta tttatcaatg tttgaaacgt aatctccacc
    13981 gaaatattgt ttacttaaac gtgccaaagt accattttct ttcaaaactt taatacgctt
    14041 atttataaat ttctgtagag ttttaccttt tttatctttt ggtaaaagga ggtattctag
    14101 tccatcctta ttattaccaa ttttaccttt caaaggagaa acgcttaagt ttaatgattg
    14161 gtcttttaca atatagtcgg atgaaatggc atcatatagg ataaagtcaa ttttcccact
    14221 ctcaatattt tttaatctgc tagtaacacc agttgtccca gaaacatatt tgatttttat
    14281 tggtttttta ttaggatgat ttttattcca attttctaga acctgtgcat agttaacgcc
    14341 agataaaact tcggttgatt ttccagagag gtcacttaat gatttgtaat ggctcccctt
    14401 cttccctact acggcataat ttgaacggga tataggatct gagaagagat atttttctgc
    14461 tctttcttta ttgtatgaaa aatcattagc tgataaatca aatttccctg catcaatacc
    14521 tgttgaaata gtatcaaaag gaactgtctt gaaggttact ttgtacttac tacctttaaa
    14581 aacagctttg acaacatcaa catcataacc tttgaatttc ccgtcttttt gataagtaaa
    14641 tggtgccgtg tcagaatctg tagctacttt aagttctact ttctctgacg cctgaactga
    14701 tactgctagg ctggcggttg cggctatagt tgcaatagat aatattattt ttttaaacat
    14761 aagaattctc ctaaaatatc atattactag tctaacactc taaaatctat ctgtataata
    14821 gtatttcctt atcaggtgat aaagatttta tatcaataaa aaagcctgaa acgagctgat
    14881 ttcagacttt aataatttta aaaactttgt tttttagctt tgcgctccgc acgcccctta
    14941 gcacgattac ttgctctacg ctctttgcga cgcttttcat caactttcca ttgaattttt
    15001 ttcttgtaac ctggttttat ttttttcttt ttctttttca caagtccaat catttctgta
    15061 tctaattttt gataggactt ttcacggtta ttacgacgat cacggtcgta tgtatcttga
    15121 aattcaccgt ttttaatgac tttaggaata aaattaatac ctagtttttc aagttcacga
    15181 atatcagaat cgtcacttgg ctgatataaa gttatagcag taccagataa gccattacgg
    15241 cctgtgcgac cgacacggtg gacaaagaaa gataggtctt gaggtatggc atcattgata
    15301 acatggctaa caccttcaat atcaatacca cgcgctgcta aatctgttgc aacgatatat
    15361 tcaaattcga ggtttttaac ctgattcata atacgtttac gttcacgagg tgcaatccca
    15421 ccatgaattt tagctacttt taagccatta ctagaaaggt acgaatgtaa ttcgtctgca
    15481 cgctctttcg tattaacaaa tatcattgca agatagggct gcattagttt agagagttcc
    15541 aaaatttgtg catttttatc acgtccttta gtggacaaaa gccagttatc gatggtatca
    15601 gcgataactg ttgctgtttt gattttttcc attacaggat tggtcaaata tttctttaaa
    15661 aatggctgta atttttgtgg aatcgttgct gaaaagacta aaatttggac atccttgggt
    15721 aaagaccctg caatcttatc aacggtatca agaaatccca tatctagagt catatctgct
    15781 tcatcaacaa caaaagtatg cgccttatga atagctaaat cacctgactt caccaaatca
    15841 taaattcgtc ctggggttcc aatgacaata tgaggttggc ttactttcaa cttttcaatt
    15901 tgacgtaatt tgtcagtacc accaacatag ttaaccaccc gtatttcttg ctctgaatgc
    15961 tcagcaattt gtttagttgc ctgataaatt tgtgttccca actctctact tggtgcagta
    16021 ataacaactt gaacatcatc actactttca tctagcttct caaaaatagg aagtaaaaag
    16081 gtgtgggttt taccagaacc agttttcgat tcccccacaa gatcgcggcc tgaacgaaca
    16141 actggaatca atttagcttg aacatcagtt ggatcgacga attttaactc atcgagagcc
    16201 ctctgaatgt atggtttaaa attaaaatct ttaaatgaca tgtctatttc cttactatta
    16261 ttatgttcat tataccatta aaggctacaa gaaagcaaaa gggaatctgg cagtccatac
    16321 cacatcccct aataaagttt aaagatataa aatagcaagt gtaatagcgc tcatgaaaat
    16381 accaatagcc cataaaaaag catctacttt ccattcgctc catttattac ctttgccaga
    16441 cacaccacct aattctaaat ggtgatgaaa tggtgtcatg cggaagatac gtttcccaac
    16501 accagttttc ttcttagtat acttaaagta tgctacttgc aacatcactg aagacgtctc
    16561 aaaaacatag acaaagccga taaaaagcaa ggtccattcc tgacgtaagg caattgaaat
    16621 ggctgctaac atagcaccca atgcaagtga accaacatct cccataaaga ctttagctgg
    16681 tttatgatta aagacaaaaa agccaagtaa agcaccaatc atgataacga taatcaataa
    16741 aatatcaaat tgcgtttgat tataagcaat gattccataa gtaatcaaac tgataacaac
    16801 cgaaatagaa gctagaccat caattccatc tgttaggttc acagcatttg aaaaaccaac
    16861 aacccagaaa agaacaaaaa aagcgtataa gtatcctact tcaaggtaaa aaccaaaaat
    16921 atttatcgca gatgtgccac ttggtaacac atggacaaaa taaaaaatta gacccgcaat
    16981 tagttggagc gacatttttt gctttggagt caagccctca ttgatttgtt taaaaatttt
    17041 caaaaaatca tctaaaaaac caataattcc atagatgaga acaacagata aaataccaaa
    17101 agttgcacct aaattaccac tattttcttt agataaaata atactaaaaa taagtgagac
    17161 aagaagagca acaatcaaaa ataccgttcc tcccattgtc ggcgtacctg ctttggctag
    17221 atgttgtttg acatcttcat gcatttgttg cccgccaatt ttcttcaatt ggtaaaactt
    17281 aatgaagcgc ggaatggcaa taactgtcag gacaaatgct atgacacctg ccattatact
    17341 taaaaacatg ttaatctcct attgtaattt tgattttttt aattttattg atggattttc
    17401 caacatcaat actttgtttt cttactttac ctgaggttga acctttatag gtcactttta
    17461 tacctgtcca tttagcaaac tgttcaacgt ttgctttaga ccaaccatac atatcaggta
    17521 attcagtcag tttatttgtt aatactagta gctgttcatt ctcagcaaga ttagctcctg
    17581 gtcttttaga tactttagaa actttactac catttcctaa aataattggc tgaaccagat
    17641 tacgacgtaa ttcttccgct acatgacctg gattttcacc aactattttt gatagtttat
    17701 attttgtctg atgttctgag tcattcaatc ccggttttaa tatcgtttct ttcattgctg
    17761 ttgcttgttc taaaacagga ttaacaacat ctttccagaa tgtgatacta aatttttcgg
    17821 gttgttggat ggtaacatac atgatgaagt caggattttc cgatggcacc atagccacaa
    17881 ctgagttaat agtatcattt tttccttgca gataaccacc accaccttcc tgggcgattt
    17941 gggcagtacc agattttact gcaactgatt gattccccac ctgaataact ggggcacctg
    18001 ctgcgtataa tgtaccataa taaggatctg tcccaacagt aaccatataa tcacgtgttt
    18061 tactagctgc ttctttggaa actggttttc caacgacttc ttttcgtgct gtacgagaag
    18121 tccctgtatt tggatcataa atactgctaa taaactgtgg ttgtaacata acaccatcat
    18181 tcgagataga cgtaaatgcg cgcaacattt gaacttgcgt tacaccaata ccttgaccaa
    18241 atgagctcat agcgatagtt acttcattgt cagatgggaa caaacctcca ctttcatgaa
    18301 gcatcccaaa acgtgttggg taaccaaatt taaacttaga aagatagttt aaccatttat
    18361 ctcgtcccat tttttgttcc aaggttacca taccaacgtt actggaatgt gcaaaccctt
    18421 gtggaaaagt catataacta ccagatgata acccttcatt gacatcccaa tctcgaataa
    18481 tcgcatctgc aattttatat tgtgcactgt tatacacttc agtagagtta aaatgcttag
    18541 aatcaattgc agaggctaga gtcattacct tcattgttga accaggttca aaaaagttat
    18601 catatagtag tgtgttatag gtacccaaat ttttcttatc gtaaccttta agggtagaag
    18661 gattataagt aggacgctga ctcgttgcta gaatttcacc cgttttagcg ttgacaactg
    18721 tagcactcgc aaattttcct ttggtttttt ctgcgaaaac atccatctga gtctctaaaa
    18781 cagtctgaat aggctctgat aaagttgtgt agatatcctt accattaacc gccctacgtt
    18841 ctgttgttgc agtacctaaa agaacattcc cactacggtc tttttcgtat gttactttac
    18901 catcagtacc tgataatacc ttgttaagtg ccgcttcaag gcctgtatta ccaactaatt
    18961 tttttccatc tccatcatcc tgaggaagag taaatcctat aaattgtgaa gcaaatatcc
    19021 catttggata aatacgcccc ggactggtag aaaaaccaat tcctttgata tctgactttt
    19081 ccatcgtttt cttgatgtca gccatcttgg tataagacaa tcctgaacct gaacttccaa
    19141 aggaaacttg aaacaatttc ttttgattga gttgttttag gacaagattt ttcttcatgc
    19201 ccaatttatt ctctaagata ctagctactt tttcatactg agaaggttgt acatatagtt
    19261 tttgacctgt agctgtcgta tagttctttg agataatagc gtagagacta taggtcgtag
    19321 catcctcagc gataggattt ccattacggt cgtaaattgt cccacgcttc gcctgtactg
    19381 tcatactttg ttgatagact ttcttggctt ccttggataa attaacccca aatttactat
    19441 ctgtaccaac gatgatgaca aagttgatga taaaaatgaa aaataggaaa atggttaaaa
    19501 tcatcagatt ttgacctact ctttccctat ttttctgcgg cgaacgccta tctcttatat
    19561 ggatcacata atcaaggaag atttttttta gctttttaaa aaaagtcaca gactagtcca
    19621 ccttccgata aatattgtcg ttttgaattg ttaaaccagc atctttagca attttagtaa
    19681 ttcgatcacg attagaaagc tcatttactt cttgtttagc attgtcaaat tctgtctgtt
    19741 tatcgttgat tttactattt aattgattta cttcttgttt aacttgtaaa ctattacttt
    19801 gcaagtaaat aataccgact gccatgataa tagcagtaat tacaatagca ccataaaaag
    19861 ctttctcaat acgagagaaa gtcttgatgt gacgttgtaa agtttgtgtt actgcttcag
    19921 ttcgtttttc atttgtcata agtttctttc ttattatcga atacgacgcg ctactctcaa
    19981 tttcgctgaa tgggcacgat tgttcgcttc tagttcttct tgacttggta agatgggttt
    20041 acgattaacg agctccatct taggttgtaa atcatctggt ataaaaggaa gacctttagg
    20101 tacttctact gttgatgctt ctttaaaaag ttgcttagta agacgatctt ctaaagaatg
    20161 aaaagtgatg acagatatac gaccatcaac agctaataag tccattgctt gttgtataga
    20221 ttcatctgca gctcctagtt cgtcattgac ttcaatacga atagcttgaa aaatttgttt
    20281 agctgggtga ccttttttct tcagttcttt ggcagattta gacgatttga taatctcagc
    20341 caattctgtt gttgtagata ttgttttttc agctcgtact tgctcaattt ttcgagcaat
    20401 ttgttttgaa aatttatctt cgccatactt aaagaaaatc cttactaaat catgataact
    20461 ataagtgttc acaacgtcat aagctgtaag actctgctct ctattcattc gcatatcaag
    20521 gggggcgtct tgtttatatg agaaaccgcg ttcacgctcg tccagctgtg ggcttgatac
    20581 acctaaatca taacaaatac cattaatttc cgacactcct aatgctttta agttagaaga
    20641 tagatttctg aaattatctt tgataaaggt tacttgaccg gtatccacat atttctttaa
    20701 acgtatatga gcattgtcaa tagccttctg gtcttggtca aatgcgtaaa ggtgcccatc
    20761 tgggcctaat tgtgaaagaa gatattcgct gtgtcctgcc ccacctaaag tagcatctac
    20821 atagatacca tccggcttaa tatcaagcat atccacagtc tcgtgcaaaa gcacagtaat
    20881 atgatgaaaa tcgtttgtca taactttaat tgtaccataa atcctaaact ttagaaaagt
    20941 aaaagagaga ccgataaatg caatctctcc tattataggt caactattcc cttacttgac
    21001 cagttccatt aatatagtat tttgtacttg ttaaagcttc taatcccatc ggtccacgcg
    21061 cgtgtaattt ttgagtcgaa ataccaatct ctgctccgag tccaaaaacg aaaccatctg
    21121 taaaacgtgt tgaagcatta acataaactg ccgctgcatc taccatatcc tgaaaaattt
    21181 ctgccctact gatattatta gtaatgatag cttctgagtg atgactactg tatttattaa
    21241 tccagctaat tgcttgttca aaagaatcta ctacttttac tgacataata tagtctaaaa
    21301 attcagtagc ataatcagac tcacttgcag caacagcatt ttctaaaagt tgcaaagctt
    21361 cctcatcggc acgaaattca actgactggt tcgattcttt gagcattttc tccaattgag
    21421 ataaaaatcc cttagcaata gcttgatgaa caactaaccc ttcggcagca ttacagacac
    21481 tagggcgttg ggttttagca ttaataacaa tcttagctgc catatctaaa tctgcaaatt
    21541 catcaatata gatatgaaca tttccaacac ctgtttcgat aacaggaatc tttgactttt
    21601 ccttcaccgt ttgaattaat tttgcaccac ctcttggaat taagacgtcc acgtagtctg
    21661 ttgcctgcat taactcctct gctacaacgt gactagtatc ttcaactaat tgtactatat
    21721 ctgggttgtg gcctgtatct tgcaatgttt ggcgcataca attaaccaag gcagtatttg
    21781 aaaaaatagc atctttacca ccacgcaaaa taatagcgtt ccctgtttta aaagctaaac
    21841 taaaggcatc caccgataca ttgggacgac tttcaaatat cattgcaata acccctaaag
    21901 gcactctttt tttaaggatt tttaaaccat ctaaatttgt ataaccacta acaacttgac
    21961 caatcggatc tgccaaatct gctacctgat aaataccgtt tgctatcgcc tgtatgcggt
    22021 ctttgtttag tcgtagacgg tccaccataa tttctgagat accattatct tttgccttaa
    22081 caacatcgcg agcattctca gtcaaaatgg tttcagtatc agccactatg tttctcgcta
    22141 agtccctgag tatctcattt ttactagctg tacttaatcg tgctactgat tgactagctt
    22201 ttttagcatt ttgccctaat atttcaatat acgtcataac tcaatctcac tttccttgag
    22261 caaaataagt tccaatatta tgcccatcta acacctttaa aatatcacga ggattggcgc
    22321 cattcatcaa tatcatttgt ccattgttat caaacaccat ttgtgcactt ttaattttac
    22381 taagcatgcc cccagtacca aatttacttc ctgctcctcc agctgacttg atgatgtcat
    22441 cggtgatttc agaaacatga ctacgtaaaa cagcatcatc ataaatatta ggatttttat
    22501 caaaaagacc atcaatatct gacagcataa ttaataaatc tgctttagta attttagcaa
    22561 cgacagcaga gagacggtca ttatccccaa acttcgtttt atgatccatc tcatccacgc
    22621 tgacagcatc attctcattg acgattggca aaattcccat agagagcaga ctctcaaaag
    22681 agtttgttac attttgcaaa ctctctggaa aaaccacaac gtcacgtgtt aataaaattt
    22741 gggaaacatg agtttgatag tgcgaaaata tctgagaata aagactcatc atggctactt
    22801 gtcctacgct agaaactgct tgttgttgtg atatttcttg aggacgctta tccattttca
    22861 aaatgtctaa accaaacccc atagcaccag atgatactaa aatcacctcc atgcctctat
    22921 tcataagact agaaataaca aatgctaaat ggtctatttt acttaaatta attttcccgc
    22981 ttgtctgtac gagggaactg gttcctactt taataacaat tcttctagtt gtctcaaaat
    23041 gtcttttcat aaaaaaatta tatcataggc cctttagaaa tgctatctga aaacatcatt
    23101 gaaaattcac aagcatttcc aagtgttttc ttttattact atgctataat ctagaatcgg
    23161 aaaacgtttt ataagtatta aggagatatt atgtctagag aatacatcac cttgtcaaaa
    23221 aatatcatca aacaccttgg tggacaaaat aatattaaca atgtttacca ttgtcaaaca
    23281 cgccttcgct ttagtctcaa tgatccgacg aaagtaaacc ttgagcaatt aaaaacttta
    23341 aaagaggtaa aaactgtcgt tatcagcgga gggcaacatc agatagtgat agggactcat
    23401 gtcgcaaaag tgtttgaaga aattaactcc ctcattgaaa ctaactcaac gactaaaata
    23461 gaacaaacca aaaaagccaa agcagttagt cgaattattg attttgtttc tgggactttc
    23521 caaccgattt tacctgcttt atcaggcgct ggtatgatta aggcattgct agctttatta
    23581 ctagtattca aaatcttgac accgtcatct caaacatata tattactaaa tctttttgca
    23641 gatggtgttt tctatttttt acctatctta atcgccatca cagctgctca aaaattgaaa
    23701 gctaatccta ttttagcttt gggaactgtt gtcatgctcc ttcaccctaa ttgggctaat
    23761 ttagtagctt ctggtaaacc agtatcctta ttccatacga tcccattcac acttactaac
    23821 tatgcatcaa gtgtcattcc cattattcta attatttgtg ttcaggctta tatagaaaaa
    23881 tatttaaagc aaattattcc taaatcactg cgtttagtgc ttgttcctat gttaatattt
    23941 ctcagcatgg gaattttgtc gttctctata ttgggaccaa tgggtactat cgctggccaa
    24001 tatttagctg ttatttttac ttttttatcc aaatacgcca gttgggcacc tgcttttcta
    24061 gtaggagcat ttgctcctat cctaatcatg tttggtgttc atagtggaat tgctgcatta
    24121 ggaatcacac aactagcgaa attaggggtt gattctattt ttggaccagg catgctctgc
    24181 tcaaatatcg cacaagcaac tgcaggtaca gtggtgactc ttataacaaa agaaaaaaaa
    24241 cttaaagaaa ttgctggacc tgctgccata acagcttata tgggaatcac cgaacctatc
    24301 ttatatggtg tcaaccttcc gaaacgttat cctctaatag cctcacttat tggtggtggt
    24361 cttggaggac tctatgccgg tataatgaac gctcaccgct tcgctgtagg ctcttctggt
    24421 ctaccaggtc tgtttcttta tatcagtcat acttcaacac atctttttat taccatgctt
    24481 attgcagtta tcattactgt aagcacaact gctatattga cattcattct agcacaatat
    24541 tacgaaaaac aagttagcat cagacaaaga gactagggat aacctagtct ctttgtctga
    24601 taagatataa tagatagcaa taaaaatcaa atattttaga gctaaaacta gcgttattaa
    24661 acaaaggatt acagtggctg ccttccaacc gtaaagtcca caacaagcct ttattttatc
    24721 cgctataact atctcagtta ttaagcatta ttcttactgt atgatttttc taaaaccttt
    24781 ttactaaaca agcttcttcc aaaaattttt tagcagtaag tattggcaaa ttgataatat
    24841 gagcaaccca atgataagga ttatgatatt gacagctaca gtattccata ccattgacca
    24901 aaaaatactt attccaataa gaaaactacc tattatcatc attccaaaca atgaaccaac
    24961 taacagccat tggcctaacc ctctattaaa tagttgcgta acctcttgcc aattcaagaa
    25021 caaatgttta tagtcacgtc tataaataaa ctggccttcg ataagtccca tgaagactag
    25081 acctaaaatg aagctcaata aaacgaggat tgatagttta agtagaaaga atcctaccaa
    25141 gaaataaata aaagtaggaa ctgcaagttg taaggttatc aaagttacaa atttatgctt
    25201 caagaaatac gaaaatgata taggcaaact ttttataaag tagaaattac ttttttctaa
    25261 agaaatacct acccctacaa tgctagctgg catcacacat aaactaccta ttaatattcc
    25321 tgctagaaaa gctatcccaa aataattttc attaaaaaat cgaccaatct ctttaccatt
    25381 attcaaaatt ggaacaatga acattgctag atataatagt ggcattaaaa atgtatttgt
    25441 caataatgtc gcattttgta aacttgataa atggtgttta acaagagttt tattaattga
    25501 ctcacgttta ctaaaatcga tttgacgatg gacttttctc tgcttgacct gtccatacaa
    25561 caactcctga tagtagcgtg gcataaccca tttataaata gcacctatca agaataaaat
    25621 gattaagagc gggagataat agttgaggag actctccata ctaaatggcg ctgtaacaat
    25681 atcataataa cctctgacat aagggattgg agcgatatct tgtaatttcc cttgtaccat
    25741 cttttgactc gtcatattca caaataataa gggaactatt gctccaaatg tagctagtga
    25801 aattaatata gtagaaataa gtttccgatt agcacttcga ataatggcac ggcctattaa
    25861 actattaata tacaatgaaa tcaccattac cgataaaaat aaaatagcaa aatttatgat
    25921 tgctaggagg accgcaagtg ggccacctat tatacgccaa taagccacaa tcattaaggt
    25981 taggattggc attagaaaag ttacagacat cccaaatgtt gcaaaaattt tagcaacata
    26041 gagctcttca gatttaatag gcaaataagc atattgttta acatcgtcac tctcatagaa
    26101 aacactgtac atcgcaataa aagagtagat aatggatact agtgtaaaga taccaatgta
    26161 gaacgtgaag tagcccggat attctttaaa atcaagtgat acaaagagaa atacataaat
    26221 tattgaaaag agcaaaatct gaaataactg atttctaaac atggatttat aagctgaaaa
    26281 ttctttttta ggatgttttt cttgcttttt tcttagtgcc gatagagtct gagggtttga
    26341 ataaaggata ttaattttta cgagttccca gatacgtgac caattcatac tttatccctc
    26401 cctactagct tgacgtcctg ctaattccaa atagatagtt tccaaatctt tatctggata
    26461 tttcattttt aactcaccta agctaccgac aaagattaat tccccttgtt tcaaaatacc
    26521 tattctgtcg cataactgtt ctgcaactgc taagacatgt gttgaaaaaa taactgtttt
    26581 tccattttta gcatgttctt tcatcatttc tttcaaatca aaagaagctt gagggtctaa
    26641 accagttaac ggctcatcta gaatccaaat gtcaggattt ggaagaaggg cacctataac
    26701 aatgactttt tgacgcatac catgtgaaaa actttcaata ggattatagc gttgctcctc
    26761 tagttcaaat atgtctacca attttgtgat acgtgcttca atatcttctg gggcaacgtc
    26821 ataaatttta gctaaaaagt accagtattc ctctgcggtt aaatttaaga aaatatctgg
    26881 cgagtccggt acataaccta tttttttctt aatttggtca cgatgttctg tcaaaagtag
    26941 gtcatcaata tagacttggc cataagttgc atcaataata gatgttagaa tactaattgt
    27001 cgttgttttt cctgctccat tgtgacctat taatccaaaa atctcaccat ctttaacaga
    27061 gagggttaaa tcactgagag cttctttttc tccgtatact ttcgaaacat gttcaaactt
    27121 aatcataaaa atctcctttt atctcctaat gatttccaaa tactttagaa agcaatcccg
    27181 ttgttacctt attttgctca tatctctcag acaatttttt agcagatttt ttcaattcct
    27241 taactgtagc taatgactcg aaagattttt ctatctcatt attttccaat tgctgagtta
    27301 actcataagc aacacgaggt acatcgctag tttttatcaa accattcgat gctgataata
    27361 gtattgatga tagcactgca tcttcacgga cagatatatc attgtaagta tgctgtatct
    27421 gccgataaaa atcacgacac tccttcttca taaacaaacc tttctaaaaa gataattaat
    27481 aatagttgta ttattttata tatacaatag ttttatcaat tatactgcgc tatggaatgt
    27541 ttgtcaaacg ttttcaaaaa agagtgcttt ccatcactta tcagtctatc aaaactattc
    27601 tcaaatccat cctatatctt acaaaaaaga agttgaggta tctttcccaa cttcttcatg
    27661 ctcttaaagt tctttatata gcgatacagc attctcaact gtaaagccat actcttcgaa
    27721 aattctgtta cctggtgctg atgctcccca agtatcaata gtaagtgtta gcccatttaa
    27781 accaacatat ttaccccaac cgaagcttga tcctgcctca atagcaagac gttttgtaac
    27841 agctgatgga agcacttgct cttgatatga agcctcttgt tcatcaaaaa tgttttgtgc
    27901 tggcatagat acgacgcgaa catggatgcc ctctgactca agtttgtctt gagtatctaa
    27961 agctaattta acttctgaac ctgttgcaat gatgatacca tcaagttcac ctttagcttc
    28021 tgataaaata taagcacctt tattaacccc ttcttgggct aattctgatg ttccttcaag
    28081 aactggtaag ttttgacgtg ttaaaacaag catcgttggt cgatctgtct ctgatacagc
    28141 tcgttgccat gcagcgtttg tttcattacc gtctgcggga cggataacgt tcaaattagg
    28201 cattgagcgc actgatgcta attgttctat tggctcatga gttggcccat cttctccaac
    28261 agcaatagaa tcatgtgtca taacatagac agttggcaag ttttgaagtg cagccatacg
    28321 tactgctggt aacaagtagt ttgagaagac aaagaacgta cccccataaa cacgagtccc
    28381 accgtgaagc gcaattccat tcattgcagc agccattgca aattcacgta caccaaacca
    28441 aatattacgc ccagcgtagt tacttgcttg gaaatctgtt tctgctgcta ccattgtgtt
    28501 attagaagct gagaggtctg ctgaccctcc ccagaggttc ggtaattgag tcgataattg
    28561 ttgaatagct tgttggcttg aaacacgact agccactgat gaacctaggt catgagcttt
    28621 cagttcaact gtctcagctt ggccagcaaa agcttctttg tattcagttg ctaattctgg
    28681 atactcaact tcatatttag caaaaagatc attccatgcc tcttctgcct tcgcaccacg
    28741 cgcttgaaga tctgaggcaa atcgatctgc aacttcagca ggaacagtaa agtctggata
    28801 ttcccatcca taagccttct tggcaaaagt aataccttct gcaccaagtg gcgctccatg
    28861 aactgaagat gttccctgtt tctctgcgcc aaatccaata atagttttaa cttcaataat
    28921 ggttggttta tcagtttcag ctttagcagc ttcaattgct gcagcaatcg cttctaaatc
    28981 attaccatct ttaactaaga tatgttgcca accatatgat tcaaaacgtc ctttaacatc
    29041 ttctgtaaat gattgagatg ttggaccatc aagtgagata tcatttgaat cataaagaag
    29101 gaccaattta ccaagtttta agtgtccagc aagactggca gcttcttggc taacaccctc
    29161 catcaaacaa ccatcaccat gaagtgtata agtgtaatga tcaactaaat caaagcctgg
    29221 tttattaaat ttagcagcca aatgagcttc agccattgcc ataccaacag cattagcaat
    29281 accttgccct aaaggtccag ttgttgcttc tacaccatca gtatggttaa cttctggatg
    29341 tccaggtgtt tttgatcccc attggcgaaa ttgttttaaa tcatcaattg ataaatcata
    29401 gccagctaaa tgaagtaggc tataaagaag agctgaacca tgcccagctg atagcacaaa
    29461 acggtcacgg tttgtccaat tgcgacttgt ttttgggttt acatttaaga atttattcca
    29521 aagcacataa gccataggcg cagctcccat aggaagacct gggtgcccag aatttgctgc
    29581 ttggatagca tcaatagaaa gcgtgcggac tgtattaacc gctaattgat caatggtgtc
    29641 aaatgtcatg ataaaacctt tctgttttta acaactaaat ttaaaattgt tcgtctttga
    29701 tattataaca taaaatacgt aaacgtttta acaatttctg gagggattaa aaccattcaa
    29761 ttaagtaatg ggattaattg tcataaataa gtgaatctat ttcctgtagt tctcgctctg
    29821 aagcaccttt attaaggcaa taggttagtt tggctttata aggtcttgag tgactctgat
    29881 aaataaccat atcaaatccc tttccttcgt cataaggttc gacttgaata gaattattac
    29941 cagagaaatg ttgtcgaatg cgatattcca acaatgactg ttccacagca ttacctgtca
    30001 tatctagacc aatcctaaag ggcatttttt gactattttt cgagaaaata agcaattcca
    30061 acaaatgtaa agtcagcaaa gaacaaccat cttctgttaa aggtctaatt cgattaacat
    30121 ttgcaataat cttctttgaa atatgatgca acttatccct catattatga tcaataagtc
    30181 gatacgtttg ttctaaatca ggttgttcaa ctaaaatatg acctttaaag aaataataat
    30241 ggctatataa ctgaccaagt tggtaaataa cctgatctga tgtctcgtca gctaaaatac
    30301 tctccttttt aaagtagatg attgtttcgg ttacacaatc aataatcggg cctccaaatc
    30361 ccatgatgta ttccattgta ttgattggta aaatattttg tgaatgtaga aaagcaaaag
    30421 ttgccagaac ttctccttcc ctatactcaa gagcaaaacg actcatatac aaaactaagg
    30481 ctgcgtctaa ccgtttataa aaagcattac attgataatg tttcacaata ggcttgagat
    30541 tatcagatat tttttccata gcaaggagac gatgatgaca aatactaaac caaagtccca
    30601 gattttgatg agcttctcga gttaaagtat gttgagccaa gcgctctatt aatagaatta
    30661 atgagtctaa gtctaatttc tttatcatat tttggcactt gtcataagac catagttgct
    30721 tgaataattc ataataaaaa taacgccatt gcaattcatc accaatctgt tttccttgtg
    30781 aaatagcaat atcaaattcc gctaacaatt cattgagatg agcaagatga cgatttaaag
    30841 tagcctcact aaccatcaat tcttgagaaa gaccaacgat tgtgaactga cgctgattaa
    30901 ataaagatat taagatagtg tatttaatac tatctttcat cagagctttc aacacttctt
    30961 gattggataa gttataatcc tcttctaaaa ataacttatc cttataataa acgattcgac
    31021 aattaacttt ttcttgttca aagtagctat taagattatt gatatattta agaaaagttg
    31081 ctcgacttaa accagtttct aataaagcta acttcagtgc tattgcctga ggagagcctc
    31141 ttaaaaaaga aagtagccga tattgaacac gcctttcttt ttccattaga gcttctattc
    31201 gcatagccca atcctctttt cataaaggtt gatagctgct tgatgcaaca tataagccat
    31261 ctcagattct ctagctgtat aaatgaaatc ttgaaaagcc agatcaaaat ctgtcatatc
    31321 acacgatata gcttgaacaa gctgattggc agtagccaaa caattttcct ttgagattaa
    31381 ttgagccccc aataaatggc ccgttttttg gttagcaatc aattttatcc ttaaatcata
    31441 ctgttcttta gggaaataag taacactaac ggtatcctcg taaaaaccag cttccaattc
    31501 agttaatcca atgttagctc tataatagcc aaagtgttta gatcctgtag cgcgtgttat
    31561 ttttaatttt ggaacagcat gtcccgataa atggtatgcc aacatttgtg ccgaacggat
    31621 tgcttggtta attaagggca tataataagc actattaaga cctttaaaat acgaccaaat
    31681 caaatcgcca atagcataga tattagggtc actcgtttgg aaataatcat ttacaacaac
    31741 actattatca acacttaaat caaccaaccc agttaaaaga cgactatccg gacgaaaatt
    31801 aacagctaat atgacagcat caactgtaaa ggtgtctcct gttagcgttc gcaaacaaag
    31861 tttttcttct cgggttacag taattttctc aaccttttga ttttggaaaa aatgtagttt
    31921 tgattcagcc atcgcatcaa gtaaaggaag ggacatatca ggatctgtat gcctgaagtc
    31981 tggtctttct tgagcttcta acaaagtgac cgccttccct gattcatgta aactataagc
    32041 aatatctaaa ccgatgatac ctgccccaac aactgctacc ttatgacttt tatctaatac
    32101 ctgcttagca ttttgactag ttgcataagt ttttgttgtt aaaacacctt gcgcatcagc
    32161 cccatcaata taagttgatt ctgcttggga gcccatggca caaatcaatt gatcataata
    32221 gacagaagaa ccatctgata acattaattc cctatcttct ttattaattt tctcaacttt
    32281 tagaccaaat agacagcgta tattctgcaa ccgttgctct tcttctgtct gccacatggc
    32341 ctgatttaaa ccactaattt cgtgacgata ataccaattt aagccatttg gaacataatc
    32401 tggatgaatt tctttatcaa tcaaaacgat atcccaatta ggatttaatt ggcgtaattt
    32461 ctgcgcacaa gtcataccag caaaagaagc tcctaaaatg acaattactt tatttacata
    32521 tttttctgtc ataattactt attataacaa aagaagttat ttttgaaaac ggtaacaaaa
    32581 aactgagacc ataaagtcgg tctcagcatc aaaaatctct tacatcatag caaatattaa
    32641 ggctgctaaa atagctccta ccataggtcc tactatcgga atccatgaat aagtccaatc
    32701 agaatcacct ttgttcttca tcggtaacaa ggcatgcaat aaacgaggac caaaatcacg
    32761 tgcaggatta atagcataac ctgttgttcc accgagcgat agaccaatcc caaccactaa
    32821 cataccaaca ctcatcgtac caactcctgg aggcatattg tattttccga tggcaaaaat
    32881 agtaagtaca agaacaagtg ttcccaatac ttcacttaaa aaattggaaa aattatcctt
    32941 caaagccgga ccagttgcaa aagttccaag gatgtctgct ctattttctg cagcctcata
    33001 atggggtcta aattgcaaat aaacaagaag tgaacctagc attgcaccaa taatctgtgc
    33061 aatcatataa agaatagctg ttccccaagc taaatcacct ttgatcgcaa aagctaatga
    33121 cacagctgga ttcaagtggg cagggctaat attgccagat accaacgctg caatagctac
    33181 agcaagtccc catccaaatg tgataacaat ccatccagaa ttatgattct tcgttttagg
    33241 caaaacaaca cctgctacaa caccattacc aagtaagact aagagagctg ttcctaagaa
    33301 ttctccaaaa atatccatta ttatctcctt caattatcct tcaaagctgc caagtcattg
    33361 tcttttagag tctcatgtaa ttcttcttca tagaaagttt tgtcttgatc agaccattga
    33421 taatgtttag ccatctcatc aataacaggc tgaatcaaag aatctaaatt atctctcatg
    33481 aaaagcatat ggttcgtacg tcttaagaag aaatccgttg ggctaagcgc catttcataa
    33541 tccatagcat agtgaagtga caacgtttct accaagctca gaccttctgc ttctgtaatt
    33601 tggcgtgtca atgcaaataa ttttggtgca tttgacccat aaaggttagc aatataacgt
    33661 gcatcctcaa tacttaaacc acttagtgtg cccaattgag cataagcttc aatttcagaa
    33721 tcaacattag atgggttaat ctctcctcct gaaacaggat aggttttaga attgattagt
    33781 ttaaacttgc gattatattc tttatccaaa atgttaataa tagtttccat ggcaccttct
    33841 gccatttttc gataatcagt aattttacca ccagcaagtg ttaataggcc attatcatca
    33901 cgctcaaaac ttgatcctcg agaaacagct gatgggtcca attctttttc agaagtacta
    33961 gattctacat gagaaatagc tttttcaaca tcctcacgat gatttttatg tgcaatataa
    34021 tctttaacgg aatcaattaa ttcttcaaaa ctttcatcgc ttagttttcc actattgcct
    34081 ccattatagt cagaggcact attccctgat agtagtggac gcaaacctgc ccatgaactc
    34141 tcaatatcat caatagtaag ctctgcttct gggaagcgtt tgtttacaat attaaggagg
    34201 tagtcaacat cttctttagt taccgtagga tgttctaagt ctccatggta atcagtatct
    34261 gtagttccaa aataagtctt atcttcacgt ggcaaaacaa aaatcatacg accatcattc
    34321 aggcctgtat caacataaac aggttgagaa atatttaatt tttggcgatc tacaactaag
    34381 tgaactccct tagttggacg taattgatga atttgtttac cttcattgga gaagttgcga
    34441 acggtgtcac tccaaggtcc agttgtatta ataacaagcc tagcttttat atcgataact
    34501 tggtcagtca acaaatcacg cgcacgtact cctataattt gattgttgtc atcaaataag
    34561 aagtcctcag ctttgacatg actggcaata taagctccat ccctattagc gcgtttaata
    34621 ttttcaatca ctaggcgtgc atcgttatta cggaaatcta aatagacccc tccgcctagc
    34681 aaaccttctt tttgtaaatc aggctcacgc ttaagaacat cttctgcgct taaaacttta
    34741 ttggctgcgg gtgtgtttgt cacacctgct aataaatcat ataaatccat agccacttta
    34801 aggcgaaaca tactaaaagt agaacccggc tcatcgtaaa ccggtaaaag cattggatct
    34861 ggcttaggaa tgtgtggtgc aatttgctgc acgactgcac gctctgatac tgtatcagaa
    34921 acaacttcta catcaaattg cttcaagtaa cgaagaccac catggaccaa ttttgttgag
    34981 cgactacttg tcccttctgc aaaatcttgc atttcaataa gtccagtatc taatccacta
    35041 gctgctgctt gtaaagcgac accagcgcct gtgatacccc cacctatgat aagtaaatcc
    35101 agagtacgat cttgcattct ttgtaaggct aaacgtcgtg tttctcgtga aaattccatc
    35161 tctacacccc cttaatcctc ttgagcaaat acttgtgtcg ctttgacagc ttttctccac
    35221 cctttataca gtttttcttt tcgagactca ttcattgttg cttggaaaag ttgtcctgtt
    35281 gcgttcagtt cttttagttc atccatactt tcccaatagc caactgaaag tccggctaag
    35341 aaagctgcac ccaaggcagt agtttcaagg ttcttcgcac gagcaatatc aatccctaaa
    35401 atatccgctt ggaattgcat taataaatta ttcattgcag ctccaccatc tacacgaagt
    35461 tgttggatat caattcctga atcaacttgc atagtgtcaa taacgtctcg tacttgataa
    35521 gcaattgact gaagtgtagc tttaacaaaa tcttctttac ttgtcccacg agttaatcca
    35581 aatactgacc ctctagcatt tgaatcccaa tacggtgcac ccaaacctgt aaaagctgga
    35641 accacataaa cctcatcgtc acttgttgat gattgagcaa ggccttctga ctcacttgac
    35701 gtttcaatca ttctaagacc atcacgtaac cattgaatag cgcttcctgc aatgaagatt
    35761 gatccttcaa gtgcataatg aaccttacca ttaatgccgt aaccaatcgt tgttaataga
    35821 ttattttgag acagttgcat ctcttcgccc gtattcatga taatgaagga acctgtacca
    35881 taagtgtttt ttaccatacc aggttcaaag gccaactgtc caaataatgc tgcctgttgg
    35941 tcaccagcca taccagaaat tggcacctct ccaccgtaaa aatggaatgg agtagttttc
    36001 ccataaactt cagaattaga ttttacttct ggtaacattg ctttaggaat atttagtaac
    36061 tctaaaattt catcatccca tttaagttct ttaatattat aaagcatcgt acgagcagca
    36121 tttgaatagt ctgtaacatg gatgagacca tcagttaact tccaaactaa ccaagtatca
    36181 attgttccaa aaagcaactc tcctttttca gcacgttcct gtgctccagg aacgtggtct
    36241 aaaatccaac gaacttttgt tgctgagaag taagcatcaa tgacaagacc agttttttca
    36301 tgaatcatgt tagtatgacc ttcttgcttc aattgatcag caatcggggc tgtttgtcgc
    36361 gattgccata caatagcatt ataaattgga agacctgttt ttttatccca aacaacagtt
    36421 gtttcacgtt gattggtaat cccaatagcc tctatctgtc caggtttgat gcttgattca
    36481 ataaaagctc cagcaatgac agattgaaca gaattccaaa tctgattagc attatgctct
    36541 acccagcctg cttgaggaaa aatttgggga aattcttttt ggctactcgc aattttttca
    36601 ccttttttat taaaaataat tgctcgagag cttgttgttc cctggtcaat cgccataatg
    36661 tatttttctt ctgaagacat aaacttcctc cttaaaagta agcgttttct ttttgtaatt
    36721 ctattatacc taaatcagtc tatcctgcac tatcaaaaat ttaatatgct cctaaaattt
    36781 ttgataagat atcatcaata acaccaaatt agccaaataa tagctaaatc aataatcatt
    36841 ccgatatacc aaactagtct gaaataccat ttagtcgttt tatgacgaaa aacttctcca
    36901 cctaggaatg caccaaatcc ccctaataat aaactctcta tcatcaatgt tttctcacta
    36961 atacgccatg cccctctaat tgcacgcctt ttatcaatag cataggttgc aaatactaaa
    37021 atattccata acagtaatgc taatagtaat tcaatcatta ttttttcctt cctaataaaa
    37081 aaggcgagac aaagattatc ttttgcccca cccaagtgtt gactttaatc taaaattgtc
    37141 tcaccattaa gaatttggat catccaaact acgaccatgt agtccttttt ctctctggac
    37201 ttgacgaagt ttttccgggg taacatcatt gccctcatca tccactaatt taattccttc
    37261 aacgtggtga cggactgatc ggcggaaacc ttcaatatac tcttcacgga gctttgcttg
    37321 ttcaaccttc tcttctccgg tcaatccaac tgtttttttc ttttttgaaa gctcgttaat
    37381 acgagcaatt tttttaggat ccataaaatg cctccttatt ttgtgtttaa taagttaaaa
    37441 gcagcgactg ttcttgcctt tgcaacaagg ctattgagta aagccagacg attttgtttc
    37501 attttttcat catctgtcat taccattgta ttatcaaaga aatcattaat aattggacta
    37561 agtgcgaaaa gcttatctaa gttgtcatgc atatcctcag tcagttcaag tgacaaaata
    37621 gcttgatata gcgccttctc ttgattattt tcaaacaatg aagagtccac caatacagaa
    37681 tgagtaactt tttcagctaa attaaatgca cgtgacaaac tttcaacgga aactttatat
    37741 tgagcatcct tgctcttttg caccaatgct tgactagctt ctagaataag gctaacaata
    37801 taagtgtctg attctaaaac ggcttctcga atatctttag ggaccttaga gccaatcatc
    37861 ttttcaatac gagctgaaat aaaggccatg acctcttttt gattagcata atctaaacta
    37921 gcaaacgata gaccataaag gttagttact aattcatcaa gtgggatatc ccaaccaaaa
    37981 gcttccaaaa tacggacaat cccctgagta gctcgacgaa gagcatatgg atcatttgaa
    38041 ccacttggaa tcaaaccaac tgagaagaat gaaagtaagg tgtcaaattt atcagctaaa
    38101 gctagaattg caccaactct ggtttctgga agctctccat ctgcagatgt cggcatataa
    38161 tgttcacgta tcgctgctgc tactgcgggc tgttcccctg caagtaaggc atatttctca
    38221 cccataatac cttgtaattc atcaaactca tcaaccattc cagttaagag gtcaaattta
    38281 taaatacttg cagctcgaag cacagctaat ttttcttcat cagaaagatc tgccttctca
    38341 gccaaatatt gtgaaataac ttttacacga tccatatgtt cataaagtga accaattttt
    38401 tcatggaaag ttacttgttt taatttctca actaaatctg caatattaag tttctgatct
    38461 tcttgccaga agaattcacc atcttcaaga cgagccacta aaactttttc attacctttt
    38521 ataacatttt cgatatgctc agcatttcca ttacgcacag aaataaagtt tggtaacaat
    38581 tttccatccc tgtcgcgaac aacaaaatag cgttgatgat ttttcattga agtgactaaa
    38641 acttcttcag gtacatcaag gtatttctcg tcaaaagaac ccaagaatgc tgtcggatac
    38701 tcaactaaat taagcacttc atttagtaaa tcttcgtcaa tttcaactga aatatttttc
    38761 ttttcttcga tagcatgaat ttgattaaca atcatttgct ggcgttcttt tgcatcagca
    38821 ataacaaatt gttgtcgcaa gtcatcttca tacgaactgg cagatgaaat ttcagtgtca
    38881 gaacccaaga aacggtgtcc tcgactaata cgccctgaat gaatatcaag gaagtctagc
    38941 tctaaggctt gatcatctaa taataccact aaagtgtgaa ctggacgaat atattcaaaa
    39001 ctattatttg cccaatgcat gttcactggg aaagtcaatt ctgttaatac ctctgtcaca
    39061 ctcgctaaaa catcaatcgc actttttcca gtttcatgct tagtgacata aacatattct
    39121 tctcccttaa cttcacgaaa ttcgatatca tcaacactta aaccttttcc gcgaacaaaa
    39181 ccttgggctg ctttagaaaa attcccttct gcatccaaag caattttctt agaaggaccc
    39241 ttgaaatctt ctgttaaatc tgtctgctgg tctgccaatc ctttcacacg aacagccaaa
    39301 cgacgtggag ttgaaaaaat ttgaacagtt tcaaatgata aacgatgatc ctctaaaaac
    39361 ttgaccattt tttggcccaa ttgcttctca ctcggtgtca caacataagc tggcaactct
    39421 tcaaggccaa gctccaataa taaatcttta gtcatttcct actccttact gtaaaacgct
    39481 tcaaatgctt ctcttaaatc ctgattcatc ttatcaagaa caacttgcct atctaaaata
    39541 tcggtacctt ctgcacgaac aacacagaaa tcatcaaaag ccataaaatc tttaaagata
    39601 gcttttaaat aatgaacagg aaattcatat ggactatact gtcctttaga ataaatagaa
    39661 cccgcagatt ctaacataag cagacgatag tcatctgtca ttaatccgac tgaaccattt
    39721 tcagtgtact tgaaagtctc acctgcaata aaaatattat caatataagt ttttgctcta
    39781 gcagtcacat tgtaattgtg cattggagaa acaaagacaa tacggtgatg tgacttaaac
    39841 tgttcaagta aatctttaga gattttagct tgaacaatct catcggctgt taattccaaa
    39901 ccctttcttt gtttagacca aatcgaaagt agtacttctt tcgtaatttc tggcaaaaca
    39961 caatcatata aatttaaaat agataactga tggttaggaa aatgctcatt ataaagttca
    40021 ataaactttt cttgtagaat agttgtaaat gagtacggat tcgaaaaatc aggatgacta
    40081 ttaacaatga gtgtgttcat tagtcttcct cagcaagtaa tttgatacga gtttcctcat
    40141 ccaaaagtgg gaaaccaagt tttttacgtt cggcaacaaa ggttttagca acaacacgtg
    40201 ctaaattccg gatacgtgca atatagcctg cacgctcagt aacagatact gctccacgtg
    40261 catctaataa attaaaggtg tgtgaacatt taaggacgta gtcataagct ggatgaacaa
    40321 gtccttcttc taaagcacgt tttgcttctc tttcaaattt ctcaaagttt tcaagtaaca
    40381 ttacttggtc tgatatctca aatgaatatt ttgaatgctc atactctggc tgagtaaaga
    40441 tttcaccata tttaacacct ggtgcccact caatatcata aacagaatct acctcttgaa
    40501 tataagaggc taaacgctct agtccataag taacttcaga agttacagga cctgtttgaa
    40561 gtccacctac ttgttggaaa taagtaaatt gtgttatttc cattccatca agccaaactt
    40621 cccaacctaa tccagctgaa cctgtagatg gattttccca gttatcttca acaaaacgga
    40681 tatcatgctc aagtggattg atacctagta attccaaaga cttcaaataa agttcttgaa
    40741 tatttgacgg cgaaggcttc attaccactt gaaattgatg atgttggtaa agacggtttg
    40801 gattttcgcc atagcggccg tcagctggac gacgtgatgg ttcaacgtat gcagcattcc
    40861 aaggctcagg gcctatagca cgtaagaaag tataagggct cattgtccct gctcctttct
    40921 cattatcata agcttgcatt aacatacagc cttggtcatt ccaaaactgt tgtaaagtta
    40981 gaataatttc ttgaaaagtt aatttcttag acatcatttt ccttctttca cactttatta
    41041 cagctgttgg gaaggtgaca acaattgact taaaacggac aaatgataaa aaccacgtcc
    41101 tacaccacct atgcccaaca gcacaggggc gtcaaagacg cggttccacc ctaatttatt
    41161 acttcatttt tgattgtact tttactcact aaaaagtgcc aatattcatc ctattctacc
    41221 caactttcac taccttgagc tcgctaaaaa aacagctgat gaacacctct tcttactgag
    41281 acagtataac aaaaaatgac ttttatgtct actatttatc ttattcttaa tataccctag
    41341 actttttgat tccatttgga ataatgatcg tttttaataa taagttttct tcacgaattc
    41401 ttaaataaat aacaaaagca taaattggca agacaaataa tgctgtgtat ttagcatgac
    41461 acaataaagt tacacctaaa agttcaggag caatatttaa gaaatagtta ggatgcttaa
    41521 caaccctaaa taaccagtga tctacaaatt gatgattttt atctaccatt aatttcactg
    41581 tccaaatacg accaagtaaa cgtgttactg tatgtaacat aaacacagaa aaaatcatca
    41641 agctaagccc gattacgcta acaaaattaa aacttgcttt tgttagaata gcctcagtta
    41701 cagatgaaaa ataaatgata atatgaagaa ctgtgattgc ttttgaaaca cctacaccat
    41761 attctttagc accatttttt cttaaggcct tttcattagc tatagatagc tttaaataag
    41821 ctaaacgaat aataaacata gcggccatta acccaataat aattgacatt gtaaactcct
    41881 attgttttgt attatattac aacattataa gaaaaaatag ttttattgtc aaactatatt
    41941 tcattaaaaa ttaataaatt aaaaagaact agtaaaaact agttctttca agattgccaa
    42001 cgtttttccc catcaaggta tgttgcaact aaatctagat gtttatctaa tacaataaaa
    42061 tccgctgcat aaccttcttt tatctgtcca cagacattat caatgcctac agacactgcc
    42121 ggaacataac tcgccatacg cactgcctct tccggagtag caattcccca ttctacaaca
    42181 ttttttaatc catctttgag cttaagaata gaaccagcta aattacctcc atctttcaaa
    42241 cgcgcagttc cattagacac aaccacgggc aattccccta acatataatc tccatcaggg
    42301 gcacctccag ctgccataca atctgttatt aatgccacat gattatggcc tttttgtgtc
    42361 attaaaatgt cacaagcaac aggatcaacg tgatgaccat cgcaaatgag ttcggcatag
    42421 gtattaggta aattataaac cgcaccaacc atacctggtt ctcggtgtgt caaaccacgc
    42481 ataccattat acgcatgtac ccaaactgag gcacccgcct tcactgcctt tttagcttct
    42541 ttatatgtcc cattagaatg acctaaagct accgtaacac cttgctttgt gattgctgag
    42601 acaaattcct caactccctc acgttctggt gccaaagcaa tttttgtaat caagcctttt
    42661 gcagccttct gccattgagc aaactcttcc aagtttggat ttctcatata aatcggattt
    42721 tgagctcctt tatattcctc agtaaaatat ggtccctcaa aatagatgcc ttgaattttt
    42781 gctccttgtt ctttgcccgc tactgccgca actgatttag acgcctcttc tagtcgcttt
    42841 gtatctgaag ttagggttgt cggcaagaaa gatgtgactc cagtagatag taagccagca
    42901 ctcattcgca aaattccttc tgaatcacaa tccataacat cagcgccagc aaaaccatga
    42961 atatgtgtat caactaaacc aggtgctatt tgataacctg aataatctaa aatctcctct
    43021 tgtccagaaa tattttctat ccatttacca aagtgattat cttttatttc taaataaccg
    43081 ttttccttta catgatcagc ataaaagaat cggtcagcct tgatgtactt cgtcatcttc
    43141 ttccccccat tcactctcat tataacgctt taaggtattt ttgtaaatgg tatataccta
    43201 ttttttgaca agtcatggtt tattctaaga aagaactgta cacttgttat aaaccacttc
    43261 acttggttgt atttttaagg ttttaattgc tgttgctcat aattcttttc cttcttcaaa
    43321 actatcaatt gctaattctc ttatagtttt tgatgagaat ggctaattaa aaagcatagc
    43381 aaaaaagtct agtaaaacta gactttcagg attttactga gcaacacctg tcccattagt
    43441 agcaggtaca gtaccagttc ctggagtcgg aacagtattg ttaacccctg gagtaactgg
    43501 tgtattattc gcttgcggtt gctcataagt tggtgctaca ttctgattaa cgtaactaga
    43561 agtatcctga ctagcaggat tagctgtccc ttgttctgta gtttcagcta ctgaactaga
    43621 gggagtttga ttcccaatca aaccatattg tgtcttaaca gtatcaatac gtttttgtaa
    43681 agcttcttta gattctttga cagttaactt attgactgct gcttcagctt ttaacacatt
    43741 ttcttctgaa ggggcttgtt ctagaatttc tactaaatct gtagcatctt tggtagcttt
    43801 cttatctgct tcgtctttaa gatacttatc ttttgctttc ttaaaacgtt tttgcaaagc
    43861 ttctttttta gaagaattgg acaatttatt taaagcaagt tcgacatcct tcaataattt
    43921 ttctgaaggt ttcttctcaa atttatctaa catatcttgt gcctcagtta attgagaatc
    43981 actgacatgg ttaacactgc tttctacagt atccatttga gtagtgccat gatctttaaa
    44041 taagaaaaac gtaacagaag caaaaattaa taaaataata ctagcaataa gtgctaaaag
    44101 ttctttttta tctattttca tactcactcc taaagtttct tcacattatt ataacataaa
    44161 ctaaaggaca taaacaaacc tattttagaa gaatcatcgt ctatcactct tattttctac
    44221 cggtaactta gttatatttg acatctcaac tactttgtat tacaatgtag ctataccagt
    44281 aacttgtatt acaatataga caaggatata atatgattcc atcgcaaatg ctaaaaggat
    44341 tacttgaagg agctatccta aaaatcatac agcaagagga gacttatgct tatgaaatct
    44401 caaaaaaatt aaattcttat ggttttggag aaatttccga agggacaatc taccccatta
    44461 tcttaagact acaaagcaat aaatatataa aaggacaaca aaagccttcc cctcaaggac
    44521 ctaagcgtaa atattatacg ttaaccaatg aaggcgtgga gcaattagcc caatttaaac
    44581 agaattggga gtcacttagt aaagcattaa acaaactact ctaaaaattg gagaggaaac
    44641 tagcatggat aaactgaaaa aattacaaaa agaaaatacg ctattagaag gaagaattga
    44701 taactctaat aaccaaacct atacagatat gattgtctat ttgcgaggtg ctagcattag
    44761 cccctaccat caggagctca ttcgaaatga tattgtcaat atgttattag aagcacaaga
    44821 gcgacaagca agtttagtta gtgtatttgg agaagatagg catgacttta taaaccaagt
    44881 tattaaatct acacctaaaa tctctaagaa agaagaaaca ttacaacgtt gggatttagc
    44941 cattttacta ctaactatcc aaatgattat ctttttagga ggatatctca ttactgaagc
    45001 acttcaacag tcagtaccag atctaatccc tattactcta ttagatgttc tttttgcaat
    45061 ctttatcagt attattgctg tcaagatagc tgataccata atatatgcta catataactt
    45121 cgataaatcg aaagaaaaga aatatttctt tcgatatata tttctaattc tttcgcttat
    45181 catagcttat atccttatag gaaaatatta tcatcttcct ttcattaata tccccctttg
    45241 gatttattta attatactgg gacttagctt ctctcttcat atcattgtta aaaaatacct
    45301 gaataaacat tattatattg aattttaaaa gaggtataac tatataaaaa ataaaacgac
    45361 aa
//
LOCUS       DATRMA010000006        45142 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.006, whole genome shotgun sequence.
ACCESSION   DATRMA010000006 DATRMA010000000
VERSION     DATRMA010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 45142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45142
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.006"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            complement(31..711)
                     /locus_tag="VBC28_000314"
     CDS             complement(31..711)
                     /locus_tag="VBC28_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HEO6814319.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSH
                     TTIYRWVQEYGKRLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDI
                     WLRKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIK
                     YLNNLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKTRKEGTLFGFSVCTEI
                     KVLLGIPA"
     gene            788..1645
                     /gene="glgD"
                     /locus_tag="VBC28_000315"
                     /pseudo
     CDS             788..1645
                     /gene="glgD"
                     /locus_tag="VBC28_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000687083.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
     gene            1642..3072
                     /gene="glgA"
                     /locus_tag="VBC28_000316"
     CDS             1642..3072
                     /gene="glgA"
                     /locus_tag="VBC28_000316"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HEO6814320.1"
                     /translation="MKIMFVAAEGAPFAKTGGLGDVIGALPKSLSKKGHDVAVVMPYY
                     DMVDQKFGDQIENLMYFYTDVGWRHQYVGVKRLSQDNVTFYFIDNQYYFYRGHVYGDW
                     DDGERFAYFQLAALELMEKIDFIPDVLHVHDYHTAMIPFLLKEKYHWIQAYNNIRAVF
                     TIHNIEFQGQFGPEMLGDLFGVGAERYEDGTLRWNNCLNWMKAAILYSDRVTTVSPSY
                     ANEIKTPEFGKGLDQIMRMEAGKLSGIVNGIDSDLLNPETDAFLPYHFSKSNLEGKIK
                     NKLALQENLGLPQDKNVPLIGIVSRLTDQKGFDIIASELDNMLQQDIQMVILGTGYHH
                     FEETFSYFASRYPEKLSANITFDLRLAQQIYAASDIFMMPSAFEPCGLSQMMAMRYGS
                     LPLVHEVGGLKDTVVAFNQFDGSGTGFSFNHFSGYWLMQTLKLALEVYNDYPEAWKKL
                     QWQAMSKDFSWDTACVAYEQLYQQLQ"
     gene            3404..3604
                     /locus_tag="VBC28_000317"
     CDS             3404..3604
                     /locus_tag="VBC28_000317"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEO6814321.1"
                     /translation="MNLAILALGFAVMGVSIGEGILVANIAKSAARQPEMFSKLQTLM
                     FTGVAFIEGTFFVLFAFTFLVR"
     gene            3637..4353
                     /gene="atpB"
                     /locus_tag="VBC28_000318"
     CDS             3637..4353
                     /gene="atpB"
                     /locus_tag="VBC28_000318"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000446422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEO6814322.1"
                     /translation="MESTSNPTVSFLGIDFDLTILAMSLLTITIIFILVFWASRKMTI
                     KPKGKQNVLEYVYELVNNTISQNLGHYTKNYSLLMFILFSFVFIANNLGLMTSLKTHE
                     HNFWTSPTANFGVDITLSLLVAFICHIEGIRKKGIGGYLKGFLSPTPAMLPMNLLEEV
                     TNVASLALRLFGNIFSGEVVTGLLLQLAVLSPFTGPLAFALNIVWTAFSMFIGFIQAY
                     VFIILSSSYIGHKVHGDEEE"
     gene            4371..4868
                     /gene="atpF"
                     /locus_tag="VBC28_000319"
     CDS             4371..4868
                     /gene="atpF"
                     /locus_tag="VBC28_000319"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000025481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEO6814323.1"
                     /translation="MSILINSTTIGDIIIVSGSVLLLFILIKTFAWKQITGIFEAREQ
                     KIANDIDTAEQARQQAEAFATKREEELSNAKTEANQIIDNAKETGLAKGDQIISEAKT
                     EADRLKEKAHQDIAQNKAEALADVKGEVADLTVLLAEKIMVSNLDKEAQSNLIDSYIK
                     KLGDA"
     gene            4868..5404
                     /locus_tag="VBC28_000320"
     CDS             4868..5404
                     /locus_tag="VBC28_000320"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEO6814324.1"
                     /translation="MNKKTQALIEQYSKSLVEVAIEHKIVEKIQQEVAALIDIFETSE
                     LEGVLSSLAVSHDEKQHFVKTLQTSCSTYLVNFLEVIVQNEREALLYPILKSVDQELI
                     KVNGQYPIQITTAVALSPEQKERLFDIAKTKLALPNGQLVEHIDPSIVGGFVVNANNK
                     VIDASVRNQLHQFKMKLK"
     gene            5420..6925
                     /locus_tag="VBC28_000321"
     CDS             5420..6925
                     /locus_tag="VBC28_000321"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEO6814325.1"
                     /translation="MAINAQEISALIKKQIEDFQPNFDVTETGIVTYIGDGIARARGL
                     DNAMSGELLEFSNGAYGMAQNLESNDVGIIILGDFSEIREGDVVKRTGKIMEVPVGEA
                     MIGRVVNPLGQPVDGLGEIETTATRPVETPAPGVMQRKSVFEPLQTGLKAIDALVPIG
                     RGQRELIIGDRQTGKTSVAIDAILNQKGQDMICIYVAIGQKESTVRTQVETLRKYGAL
                     DYTIVVTASASQPSPLLFIAPYAGVAMAEEFMYNGKHVLIVYDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERSAKVSDALGGGSITALPFIETQAGDISAYIATNV
                     ISITDGQIFLQENLFNSGIRPAIDAGSSVSRVGGAAQIKAMKRVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEVLKQPLHKPLPVEKQVVILYALTHGFLDDVPV
                     NDILAFEEALYDYFDAHYDNLFETIRTTKDLPEEAELDAAIQAFKDQSQFK"
     gene            6941..7822
                     /locus_tag="VBC28_000322"
     CDS             6941..7822
                     /locus_tag="VBC28_000322"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000919083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEO6814326.1"
                     /translation="MAGSLSEIKDKILSTEKTSKITSAMQMVSSAKLVKSEQAARDFQ
                     VYASKIRQITTNLLKSDLVSGSDNPMLSSRPVKKTGYIVITSDKGLVGGYNSKILKAM
                     MDTITDYHTENDDYAIISIGSVGSDFFKARGMNVSFELRGLEDQPSFDQVGKIIAQAV
                     EMYKNELFDELYVCYNHHVNSLTSQVRMQQMLPIKELDAEEASEDRVITGFELEPNRE
                     VILEQLLPQYTESLIYGAIIDAKTAEHAAGMTAMQTATDNAKNVINDLTIQYNRARQA
                     AITQEITEIVAGANALE"
     gene            7896..9302
                     /gene="atpD"
                     /locus_tag="VBC28_000323"
     CDS             7896..9302
                     /gene="atpD"
                     /locus_tag="VBC28_000323"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEO6814327.1"
                     /translation="MSSGKIAQVVGPVVDVVFASGDKLPEINNALIVYKNGDKSQKVV
                     LEVALELGDGLVRTIAMESTDGLTRGLEVLDTGRAISVPVGKDTLGRVFNVLGDAIDL
                     EEPFAEDAERQPIHKKAPSFDELSTSSEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYWEMKESGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSVTSIQAIYVPADDYTDPAPATAFAHLDSTTNLERKLTQ
                     MGIYPAVDPLASSSRALTPEIVGDEHYEVATEVQRVLQRYRELQDIIAILGMDELSDE
                     EKTLVGRARRIQFFLSQNFNVAETFTGQPGSYVPVEETVRGFKEILDGKHDQIPEDAF
                     RMVGGIEDVIAKAEKMNY"
     gene            9315..9728
                     /locus_tag="VBC28_000324"
     CDS             9315..9728
                     /locus_tag="VBC28_000324"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000068055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEO6814328.1"
                     /translation="MAQLTVQVVTPDGIRYDHHASLITVRTPDGEMGILPGHINLIAP
                     LNVHQMKINRSHQEGVDWVAVNGGIIEVNEDQVTIVADSAERARDIDLNRAERAKERA
                     ERALEKAQTTQNIDEMRRAEVALRRAINRISVGKK"
     gene            9793..10023
                     /locus_tag="VBC28_000325"
     CDS             9793..10023
                     /locus_tag="VBC28_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HEO6814329.1"
                     /translation="MILNIVTIVSNFIFIALFYQLFVDLFDWSKMIKMSPQNISKLKV
                     FILLISIVGGYVVSHFLLEVIQLCQSIFWALQ"
     gene            10086..11357
                     /gene="murA"
                     /locus_tag="VBC28_000326"
     CDS             10086..11357
                     /gene="murA"
                     /locus_tag="VBC28_000326"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEO6814330.1"
                     /translation="MDKIIVEGGQTQLQGQVVIEGAKNAVLPLLAATILPSQGKTLLT
                     NVPILSDVFTMNNVVRGLDIQVDFNCDKKEILVDASGDILDVAPYEFVSQMRASIVVL
                     GPILARNGHAKVSMPGGCTIGSRPIDLHLKGLEAMGATITQNGGDITAQAEKLKGANI
                     YMDFPSVGATQNLMMAATLASGTTTIENAAREPEIVDLAQLLNKMGAKVKGAGTETLT
                     IIGVDALHGTEHDVVQDRIEAGTFMVAAAMTSGNVLVKDAIWEHNRPLISKLMEMGVE
                     VSEEEDGIRVKADTKKLKPVTVKTLPHPGFPTDMQAQFTALMAVVNGESTMIETVFEN
                     RFQHLEEMRRMGLQTEILRDTAMIHGGRALQGAPVMSTDLRASAALILAGMVAQGQTV
                     VGQLTHLDRGYYQFHEKLAALGANIKRVSEA"
     gene            11359..11550
                     /locus_tag="VBC28_000327"
     CDS             11359..11550
                     /locus_tag="VBC28_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEO6814331.1"
                     /translation="MASSGWKYVLKQIGLIVLVILLALLFLAVGLMLGYSVFGDGEHA
                     YSILSLDKWQNIIGKFLGK"
     gene            11625..12482
                     /locus_tag="VBC28_000328"
     CDS             11625..12482
                     /locus_tag="VBC28_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HEO6814332.1"
                     /translation="MTKKQKQITASIVTLLFSLLAIGLGESKSLPNDHILKQVTDLII
                     SNESGKNTSLKNVSGTPSRELSESVLTSNVKKQLGTNIAWNQSGAFIINQNKTDLNAK
                     VSSAPYAINEIKKVNNQIVPTKANALLTKATRQYRNREETGNGRTYWKPAGWHQINGL
                     KGSYNHAVDRGHLIGYALVGSLRGFDASTSNPKNIATQAAWANQANSNQSTGQNYYET
                     LVRKALDRHKTVRYRVTLIYDRDNLLSSGSHIEAKSSDGSLEFNVFIPNVQSGLLFDY
                     ATGKVKQTK"
     gene            12774..13814
                     /gene="pheS"
                     /locus_tag="VBC28_000329"
     CDS             12774..13814
                     /gene="pheS"
                     /locus_tag="VBC28_000329"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000365656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEO6814333.1"
                     /translation="MDLQKQLEELKISTQEKLKEMTGNHTKELQDLRVQVLGKKGSLT
                     ELLKGLKDLSNDLRPVVGKQVNEVRDILTKAFEEQAKVVEAAKIQAQLESESVDVTLP
                     GRQMTLGHRHVLTQTSEEIEDIFLGMGFQVVDGFEVEKDYYNFERMNLPKDHPARDMQ
                     DTFYITEEILLRTHTSPVQARTMDQHDFSKGPLKMISPGRVFRRDTDDATHSHQFHQI
                     EGLVVGENISMGDLKGTLQLISQKMFGAERKIRLRPSYFPFTEPSVEVDVSCFKCGGK
                     GCNVCKQTGWIEILGAGMVHPSVLEMSGIDSEKYSSFAFGLGQERIAMLRYGINDIRG
                     FYQGDVRFTDQF"
     gene            13897..14418
                     /locus_tag="VBC28_000330"
     CDS             13897..14418
                     /locus_tag="VBC28_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO6814334.1"
                     /translation="MSQITFKKVGLDNVNILQNIAIETFRQTFSHDNSEEQLQAFFNE
                     SYTLPVLKSEITHAESDTYFVYLDTDLVGYLKVNWGSQQTEKDLDKAFEIQRIYLLDA
                     YQGQGIGKATFEFALDLAYKSGLDWAWLGVWEFNHKAQAFYAKYGFEKFSEHQFSVGD
                     KVDTDWLLRKSLH"
     gene            14472..16877
                     /locus_tag="VBC28_000331"
     CDS             14472..16877
                     /locus_tag="VBC28_000331"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEO6814335.1"
                     /translation="MLVSYKWLKELVDVDVTTAELAEKMSTTGIEVEGVETPAEGLSK
                     LVVGHIVSCEDVPDTHLHLCQVDTGDDELRQVVCGAPNVKTGINVIVAVPGARIADNY
                     KIKKGKIRGMESLGMICSLQELGLSESIIPKEFSDGIQILPEGAIPGDSIFSYLDLDD
                     EIIELSITPNRADALSMRGVAHEVAAIYGKKVHFEEKNLIEEAERAADKISVVIESDK
                     VLSYSARIVKNVTVAPSPQWLQNKLMNAGIRPINNVVDVTNYVLLTYGQPMHAFDFDK
                     FDGTTIVARNAENGEKLITLDGEERDLIADDLVIAVNDQPVALAGVMGGQSTEIGSSS
                     KTVVLEAAVFNGTSIRKTSGRLNLRSESSSRFEKGINYDTVSEAMDFAAAMLQELAGG
                     QVLSGQVTEGVLPTEPVEVSTTLGYVNTRLGTELTYTDIEEVFEKLGFAISGSEVKFT
                     VLVPRRRWDIAIQADLVEEIARIYGYEKLPTTLPEAGATAGELTSMQRLRRRVRTVAE
                     GAGLSEIITYALTTPEKAVQFSTQATNITELMWPMTVDRSALRQNVVSGMLDTIAYNV
                     ARKNSNLAVYEIGKVFEQTGNPKEDLPTEVETFTFALTGLVEEKDFQTKAKPVDFFYA
                     KGIVEALFIKLKLDVTFVAQKGLASMHPGRTATILLDGKEIGFVGQVHPQTAKQYDIP
                     ETYVAEINLSTIESQMNQALIFEDITKYPSVSRDIALLLAESVSHHDIVSAIETSGVK
                     RLTAIKLFDVYAGNNIAEGYKSMAYSLTFQNPNDNLTDEEVAKYMEKITKSLVEKVNA
                     EIR"
     gene            complement(16946..17848)
                     /locus_tag="VBC28_000332"
     CDS             complement(16946..17848)
                     /locus_tag="VBC28_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HEO6814336.1"
                     /translation="MKIDDLRKSDNVEDRRSSSGGSFSSGGSGLPILQLLLLRGSWKT
                     KLVVLIILLLLGGGGLTSIFNDSSSPSSYQSQNVSRSVDNSATREQIDFVNKVLGSTE
                     DFWSQEFQTQGFGNYKEPKLVLYTNSIQTGCGIGESASGPFYCSADKKIYLDISFYNE
                     LSHKYGATGDFAMAYVIAHEVGHHIQTELGIMDKYNRMRHGLTKKEANALNVRLELQA
                     DYYAGVWAHYIRGKNLLEQGDFEEAMNAAHAVGDDTLQKETYGKLVPDSFTHGTAEQR
                     QRWFNKGFQYGDIQHGDTFSVEHL"
     gene            18026..21259
                     /gene="rexB"
                     /locus_tag="VBC28_000333"
     CDS             18026..21259
                     /gene="rexB"
                     /locus_tag="VBC28_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent nuclease subunit B"
                     /protein_id="HEO6814337.1"
                     /translation="MKLLYTDINHDMTEILVNQAAHAAEAGWRIFYIAPNSLSFEKER
                     AVLENLPQEASFAITITRFAQLARYFTLNQPNQKESLNDIGLAMIFYRALASFEDGQL
                     KVFGRLKQDASFISQLVDLYKELQTANLSILELKYLHSPEKFEDLLAIFLVVSDLLRE
                     GEYDNQSKIAFFTEQVRSGQLDVDLKNTILIVDGFTRFSAEEEALIKSLSSRCQEIII
                     GAYASQKAYKANFTNGNIYSAGVDFLRYLATTFQTKPEFILSKWESKSGFEMISKNIE
                     GKHDFTNSSHILDDTAKDCITIWECINQKDEVEHVARAIRQKLYQGYRYKDILVLLGD
                     VDSYKLQLSKIFEQYDIPYYFGKAETMAAHPLVHFMDSLSRIKRYRFRAEDVLNLFKT
                     GIYGEISQDDLDYFEAYISYADIKGPKKFFTDFVVGAKKFDLGRLNTIRQSLLTPLES
                     FVKTKKQDGIKTLNQFMFFLTQVGLSDNLSRLVGQMSENEQEKHQEVWKTFTDILEQF
                     QTIFGQEKLNLDEFLSLLNSGMMQAEYRMVPATVDVVTVKSYDLVEPHSNQFVYALGM
                     TQSHFPKIAQNKSLISDIERQLINDANDTDGHFDIMTQENLKKNHFAALSLFNAAKQE
                     LVLTIPQLLNESEDQMSPYLVELRDIGVPFNHKGRQSLKEEADNIGNYKALLSRVVDL
                     YRSAIDKEMTKEEQTFWSVAVRYLRRQLTSKGIEIPIITDSLDTVTVSSDVMTRRFPE
                     DDPLKLSSSALTTFYNNQYKYFLQYVLGLEEQDSIHPDMRHHGTYLHRVFEILMKNQG
                     IESFEEKLNSAINKTNQEDVFKSLYSEDAESRYSLEILEDIARATATILRQDSQMTVE
                     SEEERFELMIDNTIKINGIIDRIDRLSDGSLGVVDYKSSAQKFDIQKFYNGLSPQLVT
                     YIDAISRDKEVEQKPPIFGAMYLHMQEPRQDLSKIKNLDDLVTKNHQALTYKGLFSEA
                     EKEFLANGKYHLKDSLYSETEIAILQAHNQSLYKKASETIKSGKFLINPYTEDAKTVD
                     GDQFKSITGFEADRHMARARALYKLPAKEKRQGFLTLMQQEEENDDL"
     gene            21249..24872
                     /gene="addA"
                     /locus_tag="VBC28_000334"
     CDS             21249..24872
                     /gene="addA"
                     /locus_tag="VBC28_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HEO6814338.1"
                     /translation="MTFKPFLNPEDIAVIQTEEKNSDKKQKRTPEQIEAIYTFGNNVL
                     VSASAGSGKTFVMVERILDKLLRGVPIDSLFISTFTVKAAGELKERLEKKINESLKSA
                     ESDDLKQFLTQQLVGIQTADIGTMDAFTQKIVNQYGYTLGISPIFRILQDKNEQDVIK
                     NEVYADLFSDYMTGKNAASFIKLVKNFSGNRKDSKAFREMVYKVYAFSQSTDNPKRWM
                     QTVFLKGAQTYTDFEAIPDQEVSSLLNVMQTTANQLRDLTDQEDYKQLTAKGVPTANY
                     KKHLKIIENLVHWSQDFNLLYGKKGLTNLARDITNVIPSGNDVTVAGVKYPIFKQLHN
                     RIVGLKHLEVIFKYQGESLFLLELLQSFVLDFSEQYLQEKIQENAFEFSDIAHFAIQI
                     LEENHDIRQLYQDKYHEVMVDEYQDNNHTQERMLELLSNGHNRFMVGDIKQSIYRFRQ
                     ADPQIFNDKYKAYQDNPSQGKLIILKENFRSQSEVLDSTNSVFTHLMDEEVGDILYDE
                     SHQLKAGSPRQQERHPNNKTQVLLLDTDEDDIDDSDSQQYDISPAEAKLVAKEIIRLH
                     KEENVPFQDITLLVSSRTRNDGILQTFDRYGIPLVTDGGEQNYLKSVEVMVMLDTLRS
                     IDNPLNDYALVALLRSPMFGFNEDDLTRIAIQDVKMAFYHKVKLSYHKEGHHSDLITP
                     ELSSKIDHFMKTFQTWRDFAKWHSLYDLIWKIYNDRFYYDYVGALPKAEQRQANLYAL
                     ALRANQFEKTGFKGLSRFIRMIDKVLENENDLADVEVALPQNAVNLMTIHKSKGLEFK
                     YVFILNIDKKFSMVDITSPLILSRNQGIGIKYVADMRHELEEEILPAVKVSMETLPYQ
                     LNKRELRLATLSEQMRLLYVAMTRAEKKLYLVGKASQTKWADHYDLVSENNHLPLASR
                     ETFVTFQDWLLAVHETYKKQELFYDINFVSLEELTDHHIGMVNPSLPFNPDNKVENRQ
                     SEDIVRAISVLESVEQINQTYKAAIELPTVRTPSQVKKFYEPILDIEGVDVMETITKT
                     SVDFKLPDFSTSKKQDPAALGSAVHELMQRIEMSSHVKMEDIQKALTEVNAETSVKAA
                     IQIEKINYFFQETSLGKYIQEEVEHLHREAPFAMLKEDPESGEKFVVRGIIDGYLLLE
                     NRIILFDYKTDKFVNPLELKERYQGQMALYAEALKKSYEIEKIDKYLILLGGKQLEVV
                     KMD"
     gene            24885..25802
                     /locus_tag="VBC28_000335"
     CDS             24885..25802
                     /locus_tag="VBC28_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000634642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEO6814339.1"
                     /translation="MIVEQKFGNGFTWINIEAEQLRTETSEIQAKYLDSEIITYALDD
                     YERAFMECSHIKGKEVLTIIFNTIDLKQKESYYETVPMTFCLSHDRLITVTRSRNSYM
                     LELLQKYLDRNPDVSPKKFLFAALTLITKQYFNVVSKIDREKDILNRQLREQTTNKRL
                     LAMSDLETGSVYLLTAANQNALVLEQLDVHPSQRFNSEVEKEQLSDALIEAHQLVSMT
                     QLNSQVLSQLSSTFNNVLNNNLNENLTGLNIISINLAIIAAITGFFGMNIPLPLTESR
                     SSWLIVIATSVLLWVIIAQILKRVFNLRK"
     gene            complement(25835..27211)
                     /gene="mnmE"
                     /locus_tag="VBC28_000336"
     CDS             complement(25835..27211)
                     /gene="mnmE"
                     /locus_tag="VBC28_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEO6814340.1"
                     /translation="MSITKEFDTIAAISTPLGEGAIGIVRISGTDALKIASKIYRGKD
                     LSAIQSHTLNYGHIVDPDKNEILDEVMLGVMLAPKTFTREDVIEINTHGGIAVTNEIL
                     QLILRHGARMAEPGEFTKRAFLNGRVDLTQAEAVMDLIRAKTDKAMDIAVKQLDGSLK
                     TLINNTRQEILNTLAQVEVNIDYPEYDDVEEMTTTLMREKTQEFQALMENLLRTARRG
                     KILREGLSTAIIGRPNVGKSSLLNNLLREEKAIVTDIEGTTRDVIEEYVNIKGVPLKL
                     VDTAGIRDTDDIVEKIGVERSKKALEEADLVLLVLNSSEPLTLQDRSLLELSKESNRI
                     VLLNKTDLPQKIEVNELPKNVIPISVLENENIDKIEERINDIFFDNAGMVEHDATYLS
                     NARHISLIEKAVDSLKAVNEGLELGMPVDLLQVDMTRTWEILGEITGDAAPDELITQL
                     FSQFCLGK"
     gene            27339..29249
                     /locus_tag="VBC28_000337"
     CDS             27339..29249
                     /locus_tag="VBC28_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEO6814341.1"
                     /translation="MIILQGNKIERSFSGDVLFDNINIQVDQRDRIALVGRNGAGKST
                     LLKILVGEEAPTKGEINKKRDLSLSYLAQDSRFQSENTIFQEMLQVFDSLREVEKRLR
                     ELELQMGQVSGSDLEQLMKTYDILSEEFREKGGFTYESDIKAILNGFKFNSDMWEMPI
                     SELSGGQNTRLALAKMLLEKPELLVLDEPTNHLDIDTIAWLENYLVNYQGALIIVSHD
                     RYFLDKVATVTYDLTTHSLDRYVGNYSKFMDLKAEKIATEEKNFEKQQKEIAKLEDFV
                     QRNIVRASTTKRAQARRKQLEKMERLDKPNVEQKSANMTFHAGKVSGNVVLTLENAAI
                     GYEGVSLSEPIDLDVKKFDAIAIVGPNGIGKSTLIKSLVGQIPFIKGEAKLGANVETG
                     YYDQSQSNLTKTNTVLDELWDAFSTTPEVEIRNRLGAFLFSGDDVKKSVSMLSGGERA
                     RLLLAKLSMENNNFLILDEPTNHLDIDSKEVLENALIEFDGTLLFVSHDRYFINRVAT
                     KVLEISDKGSTLYLGDYDYYLTKKAELEELARLNEEEVSASKTEIDVTSDYETQKANQ
                     KEFRKITRRVVEIEARLEVLENDENNINGLMLETNDIGKLSDLQKELESIQEEQLLLM
                     EEWENLNMRLDL"
     gene            29399..30367
                     /locus_tag="VBC28_000338"
     CDS             29399..30367
                     /locus_tag="VBC28_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000258167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEO6814342.1"
                     /translation="MVTLSKEQHLDMFLKMQRIRDVDMKFNKLVRRGFVQGMTHFSVG
                     EEAASVGAIQDLTDSDIIFSNHRGHGQTIAKGIDIGGMFAELAGKATGTSKGRGGSMH
                     LANLEKGNYGTNGIVGGGYALAVGAALTQQYEGTDNIVIAFSGDSATNEGSFHESVNL
                     AAVWNLPVIFFIINNRYGISTDITYSTKIPHLYMRADAYGIPGHYVEDGNDLMAVYEK
                     MHEVINYVRSGNGPAIVEVESYRWFGHSTADAGVYRTKEEVDSWKAKDPVKRYRAYLI
                     ENEIATEEELAAIEAQVIKEVEEGVKFAEESPFPDMSVAFEDVFVD"
     gene            30442..31440
                     /locus_tag="VBC28_000339"
     CDS             30442..31440
                     /locus_tag="VBC28_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEO6814343.1"
                     /translation="MSETKVMALREAINVAMSEEMRKDEKVFLMGEDVGVYGGDFGTS
                     VGMLEEFGAKRVRDTPISEAAIAGSAIGAAQTGLRPIVDLTFMDFVTIAMDAIVNQGA
                     KTNYMFGGGLSTPVTFRVASGSGIGSAAQHSQSLEAWLTHIPGLKVVAPGTVNESKAL
                     LKSSILDNNPVIFLEPKALYGKKEEVNMDPDFYIPLGKGDIKREGTDLTIVSYGRMLE
                     RVMQAAEEVAEEGINVEVVDPRTLIPLDKELIIDSVKKTGKLILVNDAYKTGGFTGEI
                     ATMVAESEAFDYLDHPIVRLASEDVPVPYSRVLEQGILPDVAKIKDAIYKVVNKGK"
     gene            31567..32955
                     /locus_tag="VBC28_000340"
     CDS             31567..32955
                     /locus_tag="VBC28_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase"
                     /protein_id="HEO6814344.1"
                     /translation="MAVEIIMPKLGVDMQEGEILEWKKQVGDVVNEGDVLLEIMSDKT
                     NMEIEAEDSGVLLKITHGNGDVVPVTETIGYIGAEGEEVTEASSSENTSVEENATQVT
                     SEPEKVEETSEPSVPAATSGEKVRATPAARKLAREMSIDLALVSGTGANGRVHREDVE
                     NFKGAQPRITPLARRIAEDQGVDIAEITGSGIRGKIVKNDVLAAMSPQAAEAPVETKA
                     TPTTEEKQLPEGVEVIKMSAMRKAISKGMTNSYLTAPSFTLNYDIDMTEMMALRKKLI
                     DPIMAKTGLKVSFTDLIGMAVVKTLMKPEHRYLNASLINDAQEIELHNFVNIGIAVGL
                     DDGLIVPVVHNADQMSLSDFVIASKDVIKKTQEGKLKSAEMSGSTFSITNLGMFGTKT
                     FNPIINQPNSAILGVGATIPTPTVVDGEIVARPIMAMCLTIDHRIVDGMNGAKFMVDL
                     KNLMENPFGLLI"
     gene            33015..34772
                     /gene="lpdA"
                     /locus_tag="VBC28_000341"
     CDS             33015..34772
                     /gene="lpdA"
                     /locus_tag="VBC28_000341"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEO6814345.1"
                     /translation="MAFDVIMPKLGVDMQEGEILEWKKNEGDTVNEGDVLLEIMSDKT
                     NMEIEAEDTGVLLKIVHQAGDVVPVTEVIAYIGEEGEEVGTSSPSADATITAEDGQSV
                     SGPAAPSQETVAAATPKEELAADEYDIVVVGGGPAGYYAAIRGAQLGGKIAIVEKTEF
                     GGTCLNVGCIPTKTYLKNAEILDGLKVAAGRGINLASTNYAIDMDKTVAFKNSVVKTL
                     TGGVRGLLKANKVEIFNGLGQVNPDKSVVIGDKVIKGRNVVLATGSKVSRINIPGIES
                     PLVLTSDDILDLREIPKSLAVMGGGVVGIELGLVWASYGVDVTVIEMADRIIPAMDKE
                     VSLELQKILAKKGMKIKTSVGVSEIVEANNQLTLKLNNGEEVVADKALLSIGRVPQMN
                     GLENLEPELEMERGRIKVNAYQETSIPGIYAPGDVNGTRMLAHAAYRMGEVAAENALG
                     GNKRKAHLDFTPAAVYTHPEVAMVGMTEEQAREQYGDILVGKNSFTGNGRAIASNEAH
                     GFVKVIAEPKYKEILGVHIIGPAAAELINEASTIMENELTVYDVAQSIHGHPTFSEVM
                     YEAFLDVLGEAIHNPPKRK"
     gene            34870..35859
                     /locus_tag="VBC28_000342"
     CDS             34870..35859
                     /locus_tag="VBC28_000342"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000875297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HEO6814346.1"
                     /translation="MKYIVNTSNDPAYNVALEAYAFQKLTDIDEIFILWINEPAIIIG
                     RHQNTIQEINKEFIDKNGIHVVRRLSGGGAVYHDLNNLNYTIISNNTQEGAFDFQTFS
                     KPVIDTLAKLGVKAEFTGRNDLEINGQKFAGNAQAYYKGRMMHHGCLLFDVDMSVLGQ
                     ALKVSKDKIESKGIKSVRARVTNIVDHLSDKITVQEFSDAILAQMKEEYPEMDEYVLS
                     DAELSEIQAMRDNQFATWDWTYGKAPEYTIERGVRYPAGKITTYANVENSTIKSVKIF
                     GDFFGVKPVDDIEKMLEGVRYDYKDVLAALKTVDTSQYFSRMTPEEITKAIVD"
     gene            complement(35967..36752)
                     /locus_tag="VBC28_000343"
     CDS             complement(35967..36752)
                     /locus_tag="VBC28_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HEO6814347.1"
                     /translation="MTYTSLKSPTTKDYKYTLNVAHLYGNLLNTYGDNGNILMMKYVG
                     EKLGCQMTFDIVSLEDRFDPNYYQMAFFGGGQDYEQAIVARDLPSKKEDINKFIQNNG
                     VVLAICGGFQLLGQYYIQANGERIEGIGVMGHYTLNQNNNRYIGDIKIHNDEFNETYY
                     GFENHQGRTFLSEDEKPLGTVIYGNGNNKEDGTEGVHYKNVFGSYFHGPILSRNANLA
                     YRLVATALRNKYGKEIVLPSYEEILSLEIPEEYGDVKSKADFE"
     gene            complement(36752..38095)
                     /locus_tag="VBC28_000344"
     CDS             complement(36752..38095)
                     /locus_tag="VBC28_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEO6814348.1"
                     /translation="MKINTALGVAAGKSAHYLLSKMGRGSTYPGSLALKFDKDILDTI
                     AKDYEIVVVTGTNGKTLTTALTVGILKEAFGQVVTNPSGANMITGIVSTFLTAKKSKS
                     GKKIAVLEIDEASLPRITQYIKPSLFVFTNIFRDQMDRYGEIYTTYQMILDGAANAPQ
                     ATILANGDSPLFNSKSVTNPVQFYGFNTDKHEPRLAHYNTEGILCPKCQAILTYRLNT
                     YANLGDYTCPNCDFERPNLDYALTRLTHLTNTSSGFVIDGQQYNINVGGLYNIYNALA
                     AVSVAEYFGVEPSQIKDGFDKSRAVFGRQETFTIGNKKCTLVLIKNPVGASQALDMIK
                     LAPYPFSLSVLLNANYADGIDTSWIWDANFETILTMNIPEIFAGGVRHSEIARRLRVT
                     GYDEKRIKQADKLQDIMTMIEQQETEHAYILATYTAMLEFREILANHNAIRKEMN"
     gene            38235..39086
                     /locus_tag="VBC28_000345"
     CDS             38235..39086
                     /locus_tag="VBC28_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HEO6814349.1"
                     /translation="MDIFSAIDSKFWASIMENPWMILIHLMDIIIVAVLIYKFIKALA
                     GTKIMSLIQGVILFIIIRFVSEWIGLTTITFLMNQIVTYGVIAGVVIFAPEIRTGLEK
                     FGRTPQLFTQRSQLSSDEKLVDALVKAVAYMSPRKIGALISIERTQTLQEYIATGIPL
                     DADISSELLINIFIPNTPLHDGAVIVKDKKIATACSYLPLSESSSISKEFGTRHRAAI
                     GLSENSDALTVIVSEETGGISVALKGEFLHDLSKDSFEAILRTQLIQNQEENSKLAWY
                     NQLLRRK"
     gene            39089..40048
                     /locus_tag="VBC28_000346"
     CDS             39089..40048
                     /locus_tag="VBC28_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017770882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbR-like domain-containing protein"
                     /protein_id="HEO6814350.1"
                     /translation="MKKFFTNKFWLGVVSLFLAILLFLTATATSMNHQDNSKIAGASE
                     TYTHTLTDVPIDIKYDSDDYFISGYSYGADVYMSSVNRVKLDSEINEDTRKFKVVADL
                     TNMKPGTHKVPLKVVNLPSGVNATVSPTTITVTMGKKKTKEFPVYGHVNDKQIKAGYA
                     VDKMSVDVSKVKVTSDESIIDRIDHVAANIPDDKVLDDDFNKTVTLQAVTADGTVLAS
                     IIHPSKATLSVKVKKLTKTVPINLIPVGQFSDSISKINYKLSQEKAVISGTKEALEAI
                     SVINAEVDISDVTKNTEKKINLSANNVSVDPAQVTVQLTTTKK"
     gene            40102..41454
                     /locus_tag="VBC28_000347"
     CDS             40102..41454
                     /locus_tag="VBC28_000347"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEO6814351.1"
                     /translation="MGKYFGTDGVRGEANVELTPELAFKLGRFGGYVLSQHETDRPRV
                     FVARDTRISGEMLESALIAGLLSVGIEVYKLGVLATPGVSYLVRTEKASAGVMISASH
                     NPALDNGIKFFGSDGFKLDDDRELEIEALLDAKEDTLPRPSAQGLGTLVDYPEGLRKY
                     EKFMESTGIDLEGMKVALDTANGAATASARNIFLDLNADISVIGDQPDGLNINDGVGS
                     THPEQLQSLVRENGSDIGLAFDGDSDRLIAVDENGEIVDGDKIMFIIGKYLSDKGQLA
                     QNTIVTTVMSNLGFHKALDREGIHKAITAVGDRYVVEEMRKSGYNLGGEQSGHVIIMD
                     YNTTGDGQLTAIQLTKVMKETGKKLSELASEVTIYPQKLVNIRVENNMKDKAMEVPAI
                     AEIIAKMEEEMDGNGRILVRPSGTEPLLRVMAEAPTNEAVDYYVDTIADVVRTEIGLD
                     "
     gene            41577..41948
                     /locus_tag="VBC28_000348"
     CDS             41577..41948
                     /locus_tag="VBC28_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000159232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HEO6814352.1"
                     /translation="MTKEAELLWAKHKYLVLSKSQKIYLDIRQTLKSPNCTVLDVQSL
                     IDQAVLLEESPSQVTNAYMHIWGYFKNKAERQEKEEFLTLLEKYRKTGYQRRKLLAFL
                     KQLLAKYPNSYLQNSSIFEEE"
     gene            41973..42353
                     /locus_tag="VBC28_000349"
     CDS             41973..42353
                     /locus_tag="VBC28_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6814353.1"
                     /translation="MRLWHQDIIELLPRQQLLGQHRECCALRGNGWGRKHETVDYVFR
                     YSPYRLFAYHQLVMEEMMERGYRVSKEWLIAEYRGMKCPRYDTLNPVDLETPIYPEHN
                     QDYLQECLWNLKAKGIDLPINKIK"
     gene            42445..43575
                     /locus_tag="VBC28_000350"
     CDS             42445..43575
                     /locus_tag="VBC28_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEO6814354.1"
                     /translation="MLKKPTSAYVHIPFCTQICYYCDFSKVFIKNQPVDAYLQALIRE
                     FRSYDITELRTLYIGGGTPTSISAVQLDYLLTELSRDLNLNTLEEFTIEANPGDLTVD
                     KIEVLQKSAVNRVSLGVQTFNDKHLKRIGRSHNEAQIYSTIDALKTAGFQNISIDLIY
                     ALPGQTMDDVRSNVAKALSLNIPHLSLYSLILEHHTVFMNKMRRGKLHLPTEDLEAEM
                     FEYIISEMERNGFEHYEISNFTKPGFESRHNLMYWDNVEYYGVGAGASGYLDGIRYRN
                     RGPIQHYLKGVSEGNARLSEEVLSKNEMMEEELFLGLRKKEGVSIGKFEQKFGTSFEK
                     RYGQIVQELQSDGLLKENNGFIQMTKKGLFLGDTVAEKFIVE"
     gene            43579..44316
                     /locus_tag="VBC28_000351"
     CDS             43579..44316
                     /locus_tag="VBC28_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HEO6814355.1"
                     /translation="MGLLYRETYEVPFYESDTNHYMKLPQLLALALQISAKQSLKLGI
                     GDDIVFKRYGLVWVVTDYIIDIERLPKHAEKIVIETEAKAHNKLLCYRYFYIYGEDGQ
                     KIITISSAFVLMDFKTRKIHPVLDDITSIYQSQRIKKVIRGPKYHPIGDSKVKQYHVR
                     YFDLDMNGHVNNSKYLEWMYDVLDLDFLSSHIPKKIDLKYIKEIQYGTDIKSHWYQDG
                     LVTRHDIIGGDAIHAQARIEWQEKKED"
     gene            44317..45087
                     /locus_tag="VBC28_000352"
     CDS             44317..45087
                     /locus_tag="VBC28_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HEO6814356.1"
                     /translation="MAYKGYLIDLDGTIYKGKSRIPAGERFIERLQEKGIPYMLVTNN
                     TTRTPESVQEMLRGFNVETPLETIYTATMATVDYMNDMNRGKTAYVIGEEGLKKAIAD
                     AGYVEDTKNPAYVVVGLDWNVTYDKLATATLAIQNGALFIGTNPDLNIPTERGLLPGA
                     GSLNALLEAATRIKPVFIGKPNAIIMNKALEILNIPRNQAVMVGDNYLTDIMAGINND
                     IDTLLVTTGFTTVEEVPDLPIQPSYVLASLDEWTFNEG"
     gene            45077..>45142
                     /locus_tag="VBC28_000353"
     CDS             45077..>45142
                     /locus_tag="VBC28_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HEO6814357.1"
                     /translation="MKDKLLVVLTWIWIISLATLAT"
BASE COUNT        14802 a         7220 c         9032 g        14088 t
ORIGIN      
        1 aagaataaaa tcccttacgg tatctatgat ttaagctggg attcccaata ataccttgat
       61 ttcagtacag accgaaaacc cgaagagagt gccttctttt cgggttttct tatataatcc
      121 tcggatggct tccatacctt taatcgtggt agaggcagtg cgtaaacttc gatagaattt
      181 attgcgtctc tttactggac gatggtcttg ttcaatcaaa ttattcaggt atttaatggt
      241 acgatgttct gtcccttgat aaaagccgta ttcttttagt ttcttaaagg cacttgtaat
      301 agagggggct ttatctgtga ctacaacctt cggttcatca aactgcttca ctaaccgctt
      361 aagaaaagca taggctgctt gtgtgtcccg ttttttacgt aaccaaatat ccaaggttaa
      421 accatctgca tcgatggctc gatacaaata atgccatttc cctttaattt tgatgtacgt
      481 ttcatccatt ttccatgaat aaaaggattt tttatttttc tttttccaaa tttgatagag
      541 tcgtttgcca tattcttgca cccaacgata aatcgtcgta tgagaaacgt taatgccacg
      601 atcatataag atttcttgaa cttcacgata gctaaggtta taacgaagat agtagcccac
      661 ggctacaata atcacatcct gctgaaattg ctttccttta aaatgattca tcgtcattcc
      721 tcctgctatc tttttctatt attctacctt atttgatagt agatttaaaa ctttgcaaca
      781 gaaccataca gacgatgata ctcgtacagt caatggggat tattacgatc aaattttaac
      841 ttacttgagg cgttcgggta gcgaccagac gatatatatg agttgtgata tgctttgtaa
      901 tattgactta agtcaagtaa tccacttgca taatgcaaat ggtgcgcaag ccaccgtggt
      961 ttataaaaaa atgccgagat cagctattgc agaggctaat caagtcttga ccattgatga
     1021 atctgatcat gtagtgagtc taggtaaagc tgtagataat gacgataacc aaaaaatgtc
     1081 tgctgagatt tatgttatcg atacaccttg gttaatcgaa aaaatggaag aggaagctca
     1141 aaataatgaa ccacgtaaac tccgcttctt acttcgtgat ttaattgttg aaagtaatgc
     1201 tttggctttt gaatacactg gttatttgtc aaacatttct tctattaaat cctattatga
     1261 tgctaacatg gacatgttaa caccaaataa attttattcg ctcttcattt caaaccaaaa
     1321 agtttatact aaggtgaaaa atgaggaagc tacttatttt gataaacaat caaatgtctc
     1381 taattcgcaa ctagcctctg gaagtattat taaaggttat ttagaccatt ctatcgtttc
     1441 tagaaattgt ctccttgaaa aagggactcg agtagtcaat agtattatct ttccaaaagt
     1501 aaaaatcgga gaaggggcaa ccattgaaaa tactattatt gacaaatgcg ttaaggttgc
     1561 atccggagtt acattgaaag gtagtttaga taaaccgtta gtgattccta aattttcaga
     1621 gattaatgag gatattatac aatgaaaata atgtttgttg cggcagaggg tgctcccttt
     1681 gctaaaacag gtggtttggg agatgtgatt ggcgcactcc caaaatcact tagtaaaaaa
     1741 ggtcatgatg tcgctgttgt catgccatat tatgatatgg tagaccaaaa atttggagac
     1801 caaatagaaa acctcatgta tttctataca gatgttggct ggcgtcatca atatgtagga
     1861 gttaagagac tttctcaaga taatgttacg ttctacttta ttgataatca atattatttt
     1921 tatagaggtc atgtctatgg tgattgggat gatggggagc gttttgctta tttccaatta
     1981 gctgctttag agttgatgga gaaaatcgat tttattcccg atgtcttaca cgtccatgat
     2041 tatcatacag caatgattcc tttcttattg aaagagaagt accactggat tcaggcctat
     2101 aataatataa gagcagtttt tactattcac aatattgaat ttcaagggca gtttggtcca
     2161 gaaatgttag gagatttatt tggagttggc gctgagagat atgaagatgg aactcttaga
     2221 tggaataatt gtcttaattg gatgaaagca gcaattcttt actcagatag agtgacaacg
     2281 gtatcaccaa gctacgcaaa tgaaataaaa actccagaat ttggtaaagg tttagatcaa
     2341 attatgcgta tggaagcagg caaattatct ggaattgtaa atggtattga tagcgatttg
     2401 ttaaatccag aaacagatgc ttttttgcca tatcattttt caaaaagcaa cttagagggc
     2461 aaaattaaaa ataagttggc tttacaggaa aatttgggct tacctcaaga taaaaatgta
     2521 ccattaattg gtattgtttc acgacttacc gatcaaaaag gatttgatat tattgcttct
     2581 gaattggaca atatgcttca acaagatatt caaatggtta ttttaggaac aggctatcac
     2641 cattttgaag aaacgttctc ttattttgct agtcgttacc ctgaaaaact atctgctaat
     2701 attacttttg acttaagatt agcgcaacag atttatgcgg cttcagatat cttcatgatg
     2761 ccaagtgctt ttgaaccttg tggtctctcg cagatgatgg ctatgagata tggtagcttg
     2821 cccttggtac atgaagttgg aggattaaaa gatactgtgg tagccttcaa tcaatttgat
     2881 ggtagtggta caggtttttc atttaatcat ttttcaggct attggttaat gcaaactcta
     2941 aaactagcat tagaagttta taatgattat cctgaagcgt ggaaaaagtt acaatggcaa
     3001 gctatgtcaa aagatttttc atgggataca gcatgtgttg cttacgaaca actgtatcag
     3061 caactacaat aatcacccta ttttagtgat tagttgataa gcttatcgaa tctctatgct
     3121 aaacgtttct tatgtaaaac cactttagta tagagatttt attatcctat aaggtaaaat
     3181 attattaatg taaagagaca ctatatcaaa aataaagtgt tgtcatatct taataaggga
     3241 ttaaattaaa gctaaaaaaa ctaaaaattg ttatttgaaa aggattgtgt aaaccatctc
     3301 aaactatttc atgaaaacgt aatcaaatga ggaaattcat tgacatgcac aagaaatagg
     3361 aataaaataa cggagtaaat attttttaaa ggagaattct attatgaatt tagctatttt
     3421 agcacttggt ttcgccgtga tgggtgtttc aatcggtgaa ggaattttgg ttgcaaatat
     3481 tgccaaatca gcggctcgtc agcctgaaat gtttagcaaa ctgcaaacat tgatgtttac
     3541 aggtgtggcc ttcattgaag gtaccttctt cgttctattt gcgtttacat tcttagtaag
     3601 atagacgaca atttagaaaa aaaggaggga tgaggattgg aatctacaag taatccaacc
     3661 gtatcttttt taggcattga ttttgattta accatcctag ctatgtctct cttgacaatt
     3721 actattatct ttatcttagt cttctgggct agtcgtaaga tgacaataaa accaaaaggt
     3781 aagcagaatg tcttagaata tgtctatgag cttgttaata atacaatctc acaaaattta
     3841 ggacactata ctaaaaatta tagtttattg atgtttattt tattcagttt tgtcttcatt
     3901 gctaacaact taggattgat gacatcattg aaaacacatg agcataattt ttggacatct
     3961 ccgaccgcta attttggtgt agatattaca ttatcattat tagtagcatt tatttgtcat
     4021 attgaaggta ttcgcaaaaa aggaattggt ggttatctca aaggtttctt atcaccaaca
     4081 ccagcaatgc ttccgatgaa tttattggaa gaagttacta atgttgcttc attagcattg
     4141 cgtctttttg gtaatatctt ttctggtgaa gttgtcactg gattactttt acaacttgca
     4201 gttttatcac catttacagg tccactagct tttgcattga atatcgtttg gacagcattc
     4261 tcaatgttta ttggtttcat ccaagcgtat gtgttcatta tcttgagctc aagttatatt
     4321 ggacacaaag ttcatggtga cgaagaagaa taagaaaaga gaggagaaat atgtcaatac
     4381 ttattaatag tacaacgatt ggtgatatca ttatcgtatc tggctctgtg cttctcttat
     4441 ttatcttaat taagactttt gcatggaaac aaatcacagg tatttttgaa gcccgtgaac
     4501 aaaagatagc taatgatatt gatactgctg aacaggcacg tcaacaagct gaagcttttg
     4561 caactaagcg cgaggaagaa ctatctaacg ctaaaactga agctaatcaa atcattgata
     4621 acgctaaaga gacaggtctt gcaaaaggtg atcaaattat ttcagaagca aaaactgaag
     4681 ctgatcgatt aaaagaaaaa gcacatcaag atattgccca aaataaggca gaagcactag
     4741 cagatgttaa gggtgaagta gcagatttaa cagttttatt agctgaaaaa attatggtat
     4801 caaatcttga taaagaagct cagagtaatc tcattgatag ctatattaaa aagctaggag
     4861 atgcctaatg aataaaaaga cgcaggctct aattgagcaa tattcaaaaa gccttgttga
     4921 agttgccatt gaacataaaa ttgttgaaaa aatccaacaa gaagtagcag ctttaattga
     4981 tatttttgaa acaagcgagc ttgagggtgt cctgtcaagt cttgccgttt cacatgatga
     5041 aaaacaacat tttgtgaaga cattgcaaac atcatgttca acttatcttg ttaatttcct
     5101 tgaggttatt gtacaaaatg aacgtgaagc acttctatat cctattttga aatcagttga
     5161 tcaggaactt atcaaagtga atggtcaata tccaattcaa attacaactg cagttgcttt
     5221 gtctccagaa caaaaagaac gtctttttga tattgctaaa actaaattag ctttaccaaa
     5281 tggacagtta gttgagcaca ttgacccatc tattgttggt ggatttgttg tcaatgcaaa
     5341 caataaagta attgatgcaa gtgttcgtaa tcaattacat caatttaaaa tgaaattaaa
     5401 atagaaagtg gtgtgacttt tggcaattaa tgcacaagaa attagcgctt taattaaaaa
     5461 gcaaattgaa gacttccagc caaattttga cgtcacagaa actggtattg ttacatatat
     5521 cggtgatggt attgctcgtg ctcgtgggct agataatgct atgagtggag aacttcttga
     5581 attttcaaat ggtgcgtacg gtatggctca aaaccttgag tctaatgacg tgggtatcat
     5641 tattttgggt gatttctcag aaattcgtga aggtgatgtg gtaaaacgta ctggtaaaat
     5701 tatggaagta ccagtagggg aagctatgat tggacgtgtc gtcaatccgc ttggtcaacc
     5761 agttgatgga ctaggtgaaa ttgagacgac agcaacacgc ccagttgaaa caccagcacc
     5821 tggtgttatg caacgtaaat cagtttttga gcctcttcaa acaggtctca aagcgattga
     5881 tgctttggtt cctatcggac gtggtcaacg cgaattgatt atcggtgacc gccaaactgg
     5941 taagacgtct gtagcgattg atgcaatctt gaatcaaaaa ggtcaagata tgatctgtat
     6001 ctatgtggct atcggtcaaa aagagtctac agtacgtaca caagttgaaa ctttgcgtaa
     6061 atacggtgcc cttgattata caatcgttgt gacagcttca gcatcacaac catctccatt
     6121 acttttcatt gctccatatg caggagtagc aatggctgag gaattcatgt ataacgggaa
     6181 acacgttttg attgtttatg atgatttatc aaaacaagcc gtagcttatc gtgaactttc
     6241 gcttcttctt cgccgtcctc caggtcgcga agcttaccca ggggatgttt tctaccttca
     6301 tagtcgtctt ttggaacgtt cagctaaagt atcagatgct ttaggtggag gttcgattac
     6361 agcgcttccg ttcattgaaa cacaagcagg tgatatctca gcatatattg ctacaaacgt
     6421 tatttcaatt actgacggcc aaattttctt gcaagaaaat ctctttaact caggtattcg
     6481 tccagcgatt gatgcaggtt catcagtatc tcgtgttggt ggtgcagcgc aaattaaagc
     6541 gatgaagaga gttgcaggta cgcttcgtct agatcttgct tcttatcgcg aacttgaggc
     6601 attcactcaa tttggttcag accttgatgc agcaacacaa gcaaaactaa atcgtggtcg
     6661 tcgcacagtg gaagtgttaa aacaaccatt acacaaacca ttaccagttg agaaacaagt
     6721 tgtgatttta tatgctctaa cacatggatt ccttgatgat gtacctgtaa atgatatttt
     6781 agcttttgaa gaagctttgt atgattactt tgatgctcat tatgataatc tcttcgaaac
     6841 aatccgcaca acaaaagatt taccagaaga agcagaatta gatgctgcta ttcaagcatt
     6901 taaagatcaa tcacaattta aataatagga gggttctaat atggcaggct ctcttagtga
     6961 aattaaagac aagattctct ctacagaaaa aacaagtaag attactagtg cgatgcagat
     7021 ggtctcatct gctaagttgg ttaaatctga acaagctgct agagattttc aagtgtatgc
     7081 ctctaaaatt cgtcaaatta ctactaacct cttaaagtca gacttagtat ctggatcaga
     7141 taaccctatg ttatcttcac gtccggtgaa gaaaacaggt tatattgtta ttacttctga
     7201 taagggttta gttggtggtt acaattcaaa aatcttaaaa gcgatgatgg acactataac
     7261 tgactatcat actgagaatg atgattatgc catcatttca attggtagtg ttggttctga
     7321 ttttttcaaa gcacgtggaa tgaatgtttc tttcgaatta cgtggtttag aggaccaacc
     7381 aagttttgat caagttggta aaattattgc tcaagcagtt gaaatgtaca aaaatgagtt
     7441 gttcgatgaa ttatatgttt gctacaatca ccatgttaac agtttaacta gccaagtcag
     7501 aatgcaacaa atgcttccta tcaaagaatt ggatgcagaa gaggcttctg aggatagagt
     7561 gattactggt tttgaacttg agccaaaccg tgaagttata ttggagcaat tattgcctca
     7621 atatactgag agtttgattt atggagctat tattgatgct aaaacagcag agcatgcggc
     7681 tggtatgaca gctatgcaga ctgcaactga taatgctaaa aatgttatca atgatttaac
     7741 tattcaatat aaccgcgctc gtcaagcagc tatcacacag gaaattacag aaattgttgc
     7801 aggtgctaat gcacttgaat aattaattag tctatagaaa aaatagtgaa caaaggtatt
     7861 ttaaatcctt tgtatcatat gataaggaga aatacatgag ctcaggcaaa attgctcagg
     7921 tagttggacc tgtcgttgac gttgtatttg caagtggcga caaacttcct gagattaata
     7981 atgcattgat tgtctataaa aatggcgata agtcacaaaa agtagtactt gaagttgctc
     8041 ttgaacttgg tgacggcctc gttcgtacaa tcgctatgga atcaactgat gggcttacac
     8101 gtggtttgga agtattagat actggtcgcg caattagtgt gccggttggt aaggatactt
     8161 tgggtcgtgt cttcaacgtt cttggagatg ctattgacct tgaagagcct tttgcagaag
     8221 atgcagaacg tcaaccaatc cataaaaaag caccatcgtt tgatgaatta tcaacatcat
     8281 cagaaatctt agaaactggt attaaagtta ttgacttatt agcaccttac ttaaaaggtg
     8341 gtaaagttgg acttttcggt ggtgcaggtg ttggtaaaac cgttcttatt caagaattaa
     8401 tccacaacat cgcccaagaa catggtggta tttcagtatt tactggtgta ggagaacgta
     8461 ctcgtgaagg gaatgacctt tattgggaaa tgaaagaatc tggcgttatt gaaaaaacgg
     8521 ctatggtctt tggtcaaatg aatgaaccac caggagcacg tatgcgtgtg gcacttactg
     8581 gtcttacaat agctgagtac ttccgtgatg tagaaggaca agatgtgctt ctcttcattg
     8641 ataatatctt ccgtttcaca caagctgggt cagaagtgtc agcgctttta ggtcgtatgc
     8701 cttcagccgt tggttatcaa ccaacacttg ctacagaaat gggacaatta caagagcgta
     8761 tcacttcaac taaaaaaggt tctgttacat caatccaagc tatttatgtg ccagcagatg
     8821 actatactga ccctgcgcca gctacagcct ttgctcactt agattcaaca actaaccttg
     8881 aacgtaaatt gacacaaatg gggatttatc cagccgttga cccattagct tcaagttctc
     8941 gtgcactcac accagaaatc gttggagatg agcactatga agttgcaaca gaagtacagc
     9001 gtgttcttca acgttaccgt gagttacaag atatcattgc gattttaggt atggatgaat
     9061 tatcagatga ggaaaaaacg ttagttggac gtgcacgtcg tatccaattc ttcctttctc
     9121 aaaacttcaa tgttgcagaa acatttactg gtcaaccagg ttcatatgtt ccggttgaag
     9181 aaactgttcg cggattcaaa gaaatcctag atggtaaaca tgatcaaatt ccagaagatg
     9241 ccttccgtat ggttggtggt attgaggatg ttatcgcaaa agcagaaaaa atgaactatt
     9301 aatgaggtga tgctatggca caattgactg ttcaagtagt aacaccagat ggtattagat
     9361 atgaccatca cgctagtctc attactgtta gaacgccaga tggtgaaatg ggaattttac
     9421 caggtcacat aaatttgatt gctcccctta atgttcatca gatgaagatt aatcgttctc
     9481 atcaagaagg tgtagactgg gtagctgtta atggtggtat aattgaagtt aatgaggatc
     9541 aggtgacaat tgttgcggat tcagcagaac gagcacgtga tatagacctt aaccgtgcag
     9601 aacgggctaa ggaacgcgct gagcgtgcat tagaaaaagc tcaaacaact cagaatattg
     9661 atgagatgcg tagagctgag gttgctcttc gtcgcgcaat taaccgtatt agcgtcggta
     9721 aaaagtagaa aaatggtcta attagttttg tgactaatta ggctttttta gtgtataata
     9781 gaaggcatga atatgatatt aaatatagta actatagtta gtaattttat ttttattgcc
     9841 cttttttatc aactttttgt agatttattt gactggtcta aaatgattaa aatgtcccct
     9901 caaaacatct caaaattaaa agtttttatt ttgttgataa gtatagttgg tggctatgtg
     9961 gttagtcatt ttttattgga agtgattcag ttgtgtcaat caatcttttg ggctctccaa
    10021 taaaggaaat attagctgaa ctatgatata atcaacttac tattgttata agaatggaga
    10081 ttcaaatgga taaaattatt gtggaaggtg ggcaaacgca gcttcaaggt caagttgtta
    10141 ttgaaggagc taaaaatgca gttttacctc ttttagcagc cacaatttta ccaagccaag
    10201 ggaaaacatt gttgaccaat gttcctatct tgtctgatgt atttacaatg aacaatgttg
    10261 ttcgtggttt agatattcaa gttgacttta attgcgataa aaaagaaatc ttagtggatg
    10321 cctcaggtga tattttagat gttgcacctt atgaatttgt tagtcaaatg agagcttcta
    10381 ttgttgtttt gggacctatt ttagctagaa atggccatgc taaggtttca atgccaggcg
    10441 gttgtacaat tggtagccga ccaattgacc ttcatcttaa aggtttagaa gctatgggag
    10501 ctacgattac acaaaatggt ggtgatatta ctgcccaagc tgaaaagctt aagggagcta
    10561 atatatacat ggattttccc tcagtcggtg ccactcaaaa cttaatgatg gctgctacac
    10621 ttgcttcagg cacaacaacg atagagaatg ctgcacgtga accagaaatt gtagaccttg
    10681 cgcagttact aaataaaatg ggagctaaag taaagggtgc tggtacggaa acattaacga
    10741 tcattggtgt tgatgcttta catggtacag aacatgatgt tgtccaagat cgtattgaag
    10801 ctggaacctt tatggtggca gctgctatga cttctggaaa tgttctagta aaggatgcta
    10861 tttgggaaca taaccgtcct ttaatctcta aattgatgga gatgggtgtt gaagtgtcag
    10921 aggaagaaga tggtattcgt gtaaaagctg atactaaaaa attgaagcca gtgactgtga
    10981 aaacactccc tcatccagga tttcctactg atatgcaagc tcaatttaca gctctaatgg
    11041 cagtagttaa tggcgaatca actatgattg agacagtttt tgaaaatcgt tttcaacatt
    11101 tggaagaaat gcgtcgcatg ggattacaaa cggaaatttt acgagatact gcaatgattc
    11161 atggtgggag agctttacaa ggtgcaccgg tcatgtctac tgacttacga gctagtgcag
    11221 ctttaatctt ggcaggaatg gttgcgcaag ggcaaacagt agttggccaa ttaacccatt
    11281 tagaccgtgg ttattatcaa ttccatgaaa aattagcggc tcttggtgct aatattaaac
    11341 gtgtaagtga ggcgtaatat ggcatctagt ggttggaaat atgtattaaa acaaatcggt
    11401 ctaatagtat tagtcatact attagccttg ctgtttttag cggttggatt gatgttgggt
    11461 tatagtgttt ttggtgacgg agagcatgct tactctattt tatcgctgga taaatggcaa
    11521 aatattattg gaaaattttt aggaaaatag aaagagccac tataagttat ttgatgctta
    11581 tagtggtttc ctttgagagt taattttagc agtagaataa tacaatgaca aaaaaacaaa
    11641 aacaaataac agcttcgatt gtaactttgc ttttttcatt actggcaata gggttaggtg
    11701 aaagcaaatc tttgcctaat gatcacattt taaaacaggt gacagattta attattagta
    11761 atgaatctgg caaaaatact tcccttaaaa atgtatcagg aactcctagt agagagttat
    11821 ctgaatctgt tttgactagc aatgttaaaa aacaattggg aacaaatata gcttggaacc
    11881 agtcaggtgc ttttatcatt aatcaaaata aaacagattt gaatgctaaa gtgtcaagtg
    11941 caccatatgc tattaatgaa ataaagaagg ttaataacca aattgttccg actaaagcaa
    12001 atgccttatt aacaaaagca actcgtcaat atcgaaatcg agaagaaaca gggaatggta
    12061 gaacttattg gaaaccagct ggatggcatc agataaatgg attaaaaggg agctataacc
    12121 acgctgtgga cagaggccat ttaattggtt atgctttagt agggagttta agaggatttg
    12181 atgcctccac aagtaatcct aaaaatattg ccacacaagc agcttgggca aaccaagcta
    12241 atagcaacca atcaacaggg caaaattatt atgaaacttt agttcgtaaa gcattggatc
    12301 gtcataaaac tgttaggtat cgtgtgactt taatttatga cagagataat cttttatctt
    12361 cgggatcaca tattgaggct aaatcttcag atggaagttt agaatttaac gtttttattc
    12421 ctaatgtaca atcgggatta ttatttgatt atgcaacagg aaaagtgaag cagacaaagt
    12481 aatctttatc taatagtgct tgttaaagac tgaaaaattt gataaaatag tccttaattg
    12541 aatagaggtc tgtgagacta gtaaaccaag ggaagctgct tagggagttc agccgtgact
    12601 gcaagctgaa tcagtgaaag agggtggaat tcactcacaa ataggactat aattaaggtc
    12661 tgattggtcg atcagataga aaacggatgg taccgcgtgt caacgctccg attaaggggt
    12721 agtggcacgc ttttttttgt tttcaaagct acttattttg acttggagga aagatggatt
    12781 tacaaaaaca attagaagag ttgaaaatat caactcaaga aaagcttaaa gaaatgactg
    12841 ggaaccacac taaagaatta caagacttac gtgtacaagt tttaggaaaa aaaggttctc
    12901 taacagaatt actaaaaggt ctgaaagatt tatctaatga tttacgacca gttgttggaa
    12961 aacaagtcaa tgaagtacgt gatattttaa caaaagcgtt tgaggaacaa gctaaagtag
    13021 tggaagctgc gaagattcaa gcacagttag agtctgagag tgtcgacgtt accttgcctg
    13081 gtcgtcaaat gacattaggt caccgtcatg tcttaacaca aactagcgaa gagattgaag
    13141 atatcttttt aggtatggga ttccaagtgg ttgatggttt tgaagttgag aaagactact
    13201 ataattttga acgaatgaat ttaccaaaag atcatccagc ccgtgatatg caagatactt
    13261 tttatattac agaagaaatc ttgctacgta ctcatacttc gcctgttcaa gcacgaacaa
    13321 tggaccaaca tgatttttct aaagggcctt taaaaatgat ttctcctgga cgtgttttcc
    13381 gtcgtgatac tgatgatgct actcatagcc atcaattcca tcaaattgaa ggcttagttg
    13441 ttggagaaaa tatttcaatg ggcgatctca aaggaacact tcaactgatt tctcaaaaaa
    13501 tgtttggagc agagcgtaaa atccgacttc gtccttcata tttcccattc acagaacctt
    13561 cagttgaagt tgatgtctct tgcttcaagt gcggaggtaa gggatgtaat gtctgcaaac
    13621 agacaggttg gattgagata ttgggtgctg gtatggttca cccaagtgtt ttggaaatgt
    13681 ctggtattga ttctgagaaa tactccagtt ttgcctttgg tttaggtcaa gaacgtattg
    13741 caatgttacg ctacggtatc aatgacattc gtggcttcta tcaaggtgat gttcgcttca
    13801 cagatcaatt ttaagatttt agatagcaag tagcatcatt ggtgtgatga taaatttatg
    13861 ttaacgctat taccttactc aataggaggt tttctaatgt ctcaaattac ttttaaaaag
    13921 gttggccttg ataatgtgaa tattttacag aatattgcta tagaaacctt tcggcagacg
    13981 ttttctcatg ataatagtga ggaacaactt caagcatttt tcaatgagtc ttacacttta
    14041 ccagtattaa aatcggaaat tacccatgct gaatctgata cttattttgt ttatcttgat
    14101 acagatttag tgggttattt aaaagtaaac tggggaagtc agcaaactga aaaagatctt
    14161 gataaagcat ttgaaattca aaggatttat cttctagatg cctaccaagg gcaaggtata
    14221 ggtaaggcta ctttcgaatt tgctttggat ttagcttata aatcaggctt agattgggct
    14281 tggttaggtg tttgggagtt taatcataaa gctcaggcat tttacgctaa atacggattt
    14341 gaaaaatttt ctgagcacca attttcagta ggtgataaag tggatacgga ttggttatta
    14401 agaaaaagtt tacactaaaa actacaaagc acttgtataa aaataaaaga aaataaatag
    14461 agagaaatta catgttagta tcgtataaat ggttaaaaga gttagtggat gttgatgtta
    14521 caacagcaga actagctgag aaaatgtcaa ccactggtat tgaggttgaa ggtgtggaga
    14581 caccagcgga aggtttatca aaattagttg taggtcatat tgtttcgtgt gaagatgttc
    14641 cagataccca cttacatttg tgtcaagttg atacagggga tgatgaactt cgtcaagttg
    14701 tctgtggtgc tccaaatgtc aagactggta ttaatgttat cgtagcggtt cctggtgctc
    14761 gtattgcaga taactataag attaaaaaag gtaaaatccg tggtatggag tcacttggaa
    14821 tgatttgttc acttcaagag ttgggcttat cggaaagtat cattccaaaa gagttttctg
    14881 atggtatcca aattttacct gagggagcta tacctggtga tagtattttc tcttatcttg
    14941 acttagatga tgaaattatt gaattatcaa ttacaccaaa ccgtgcagat gctctttcaa
    15001 tgcgtggtgt tgcccatgag gtagcagcaa tttatgggaa aaaagttcat tttgaagaaa
    15061 aaaatctcat tgaagaagca gaaagagcag ctgataagat ttctgttgtc attgaatcag
    15121 ataaagtttt atcttactcg gctcgtattg ttaagaatgt aacagttgca ccaagtccac
    15181 aatggcttca aaataaactg atgaatgctg gtatacgtcc aattaataat gttgttgatg
    15241 tgacaaacta tgttcttttg acttatggtc aaccaatgca tgcttttgat tttgataaat
    15301 ttgatgggac aactatagta gcacgtaatg ctgagaatgg ggaaaagtta attactcttg
    15361 atggtgaaga gcgtgactta atagctgatg acttggtcat tgcggtaaat gatcaaccag
    15421 ttgctttggc aggcgttatg ggaggacaat caactgaaat tggtagttct tctaagacag
    15481 tggtccttga agcggcagta tttaatggaa caagtatccg caaaacaagt ggaagactca
    15541 accttcgttc agaatcatct tctcgctttg aaaaaggtat caattatgat acagtttcag
    15601 aggctatgga ttttgcggct gcaatgttac aagaattggc aggtggtcaa gttctttctg
    15661 gtcaagttac agaaggagtt cttccaactg agccggtaga ggtatcaacg actcttggtt
    15721 acgttaatac tcgtttggga acagaactta cctatactga tattgaagaa gtatttgaaa
    15781 aactcggctt tgctatttct ggttcagaag tgaaatttac tgtgttggta ccgcgtcgta
    15841 gatgggatat tgccattcaa gctgacttag ttgaagagat tgcccgtatt tatggttacg
    15901 aaaaattacc aacaacattg ccagaagccg gagcgactgc aggagaatta acgagtatgc
    15961 aacgcttacg tcgtagagtc cgtacggttg ctgaaggagc aggattatct gaaattatca
    16021 cctatgcttt aacaactcca gaaaaagcag tgcagttctc aactcaagca actaatataa
    16081 ctgaacttat gtggccgatg actgttgatc gctcagcact tcgccaaaat gttgtttcag
    16141 gaatgcttga tacaattgct tataacgttg ctcgcaaaaa cagtaacctt gcagtttatg
    16201 agataggaaa agtttttgaa caaactggta atccaaaaga agatttacca actgaagtag
    16261 agacgtttac ttttgcctta acaggtctag tagaggaaaa agatttccaa acaaaagcca
    16321 agccagttga tttcttctac gccaaaggta ttgtagaggc gctctttatt aaattaaaat
    16381 tagatgttac ttttgtagca caaaaaggtt tagcaagtat gcacccaggt cgtacagcaa
    16441 ctatcttatt agatgggaaa gagattggtt ttgttggtca agtacaccca caaactgcaa
    16501 aacaatatga tattcctgaa acttatgttg ctgaaatcaa tctatcaact attgaaagtc
    16561 aaatgaacca agcgttgatt tttgaagata ttacgaagta tccgtctgta tctcgcgata
    16621 ttgcattatt acttgcagaa tctgttagtc atcatgatat tgtgtcagca attgagacat
    16681 caggggtaaa acgtctaaca gcgattaaac tgtttgatgt ctacgctgga aataatattg
    16741 cagaagggta taaatcaatg gcttatagct taactttcca aaaccctaat gacaatctaa
    16801 cggacgaaga agtggctaaa tatatggaaa aaatcactaa atcactcgtt gagaaagtta
    16861 atgcagaaat aagataattt atattttacg ttgaaaaata aataaaaaac agcatcaatt
    16921 aagatgctgt ttttttattt aaaatttata gatgttctac ggagaaagta tcaccgtgtt
    16981 ggatgtcacc atattgaaag cctttgttaa accaacgttg gcgttgttca gctgttccat
    17041 gggtaaagct atcaggcact aattttccgt aggtttcttt ctgaagggta tcgtctccga
    17101 cggcgtgggc agcattcatg gcctcttcaa agtctccttg ttctaagaga ttttttcccc
    17161 tgatgtagtg agcccatacc cctgcataat aatctgcttg aagttctagc cgaacattta
    17221 aagcatttgc ttctttctta gtaagtccgt gtcgcattct attatactta tccataatgc
    17281 ctaactctgt ttgaatgtgg tgaccaactt cgtgggcgat gacgtaggcc atagcaaaat
    17341 caccagtagc accatattta tgtgataatt cattgtaaaa agaaatatca agatagattt
    17401 ttttatctgc tgaacaataa aatggtcctg aagcagattc acctatacca caacctgttt
    17461 gaattgaatt ggtgtaaaga acaagttttg gttccttata atttccaaaa ccttgggttt
    17521 ggaattcttg tgaccagaaa tcctcagttg agccaaggac tttattaacg aaatcgattt
    17581 gttctctcgt tgcgctatta tcaacagaac gtgagacatt ctgagattgg taactagaag
    17641 gtgaggatga gtcattaaaa atgctggtta gtccccctcc gccaagtagc agtaagatga
    17701 ttaaaaccac aagcttggtt ttccaactcc ctcgcagcaa taaaagttga agaatcggta
    17761 atccacttcc tccgctagag aatgaacctc cgctactgga gcgacgatct tcaacattgt
    17821 cgctttttct taggtcatct attttcataa gtatctccaa agtgttttat aggctcatta
    17881 taaactattt taataagaaa tgcttgaatt tttaactaaa tatcttcccc tagatttgaa
    17941 gctatttttg gtataatatt aataaactgt aaaggataag ttgaagaagg tttacttctc
    18001 agttgactta taagatagga gattcatgaa attactttat actgatatta atcatgatat
    18061 gacagaaatc ttagtcaatc aggccgcgca tgctgctgaa gctggatggc gtatttttta
    18121 tattgctcca aactctttat cttttgaaaa agagcgtgca gtattagaga accttcctca
    18181 agaagcctct tttgctatta caattactcg ctttgctcaa ttggcacgtt attttacttt
    18241 aaaccagcct aatcaaaaag aaagtttaaa cgatattgga ttagcaatga tcttttatcg
    18301 tgctttagca agttttgaag atggacaatt aaaagttttt ggacgattga agcaagatgc
    18361 ttcctttatc agtcaattgg tagacctata caaagaattg cagacagcaa atctgagtat
    18421 tttagagtta aaatatttac attctcctga aaaatttgaa gatttattag ctatttttct
    18481 agtggtttca gatcttttaa gagaaggaga atacgacaat caatctaaaa ttgccttttt
    18541 tacagaacaa gtaaggtcag gccagctaga tgtggattta aagaacacta tccttatagt
    18601 cgatggtttt acccgtttct cagctgaaga agaagcttta attaaaagtc tgagttcacg
    18661 ttgtcaagag attatcatcg gagcttatgc tagccaaaaa gcgtataagg ctaactttac
    18721 taatggaaat atttattctg caggggttga ttttttaaga tatctagcaa cgacatttca
    18781 aacaaaacca gaatttatct tgtccaagtg ggaaagtaaa tctggttttg aaatgatttc
    18841 gaaaaatatc gaaggtaaac atgattttac aaatagtagt cacattttag acgacactgc
    18901 taaagattgt ataacaattt gggagtgcat taatcaaaaa gatgaagtgg aacatgtggc
    18961 tagagctata cgtcagaaac tttatcaagg atatcgctac aaagatattt tagttttact
    19021 aggagatgta gatagttata aactacaatt aagtaagatt tttgagcagt atgatattcc
    19081 ttattatttc ggtaaagctg aaacaatggc tgcgcaccct ttagttcatt ttatggattc
    19141 cttgtctcga ataaaacgtt atcgatttag agctgaagat gttttaaatc tttttaagac
    19201 aggtatttat ggagagatta gccaagatga tttagattat tttgaggctt atatctcata
    19261 tgcagacatt aaaggtccta agaagttctt tacagatttt gttgtggggg ctaaaaaatt
    19321 tgatttaggg cgtcttaaca ctattcgtca gtccctcctt actccactgg aaagttttgt
    19381 gaaaacgaaa aaacaagacg gtataaaaac actcaaccaa ttcatgtttt tccttactca
    19441 ggtaggtttg agtgacaatt tatcgcgatt agttggtcaa atgtctgaaa atgaacaaga
    19501 aaaacatcaa gaagtttgga aaacatttac agatatttta gagcagtttc aaacaatatt
    19561 tggtcaagaa aaattaaatt tagacgaatt tttatcgtta ttaaattcag gtatgatgca
    19621 agccgagtat cgtatggtcc ctgctactgt tgatgtggtt actgttaagt cttacgattt
    19681 ggtagaacca catagtaatc aatttgtcta tgccctaggc atgacacaat cacattttcc
    19741 aaagattgct caaaacaaat ctttaatatc tgatattgaa cgtcagctta taaatgatgc
    19801 caatgacact gatggtcatt ttgacattat gacgcaagaa aatttgaaga aaaaccattt
    19861 tgctgccctc tctcttttta atgctgctaa acaagaatta gtgttgacta taccacaatt
    19921 attgaatgag tctgaagatc agatgtcacc atatcttgtc gaactaagag atataggtgt
    19981 tccttttaat cataaaggac gccaatcgtt aaaagaagaa gctgataata ttgggaatta
    20041 caaagcttta ttatcgcgtg ttgttgactt gtatcgctct gctattgaca aagagatgac
    20101 taaagaagaa caaacttttt ggtcagttgc tgttcgttat ttgcgtcgtc aattaacttc
    20161 taaagggatt gagataccaa taattacaga tagtcttgat actgtgacag tttcatcaga
    20221 tgtgatgacc agacgcttcc cagaagatga cccacttaaa ttatcctcat ctgctttgac
    20281 aactttttat aataatcaat ataagtattt tttacaatat gtgttaggtt tggaagaaca
    20341 agactcaatt catccagata tgcggcacca tggaacgtac cttcatcgtg tttttgaaat
    20401 tctgatgaaa aaccaaggga tagaatcttt tgaagagaaa ctcaactctg caattaataa
    20461 aaccaatcaa gaagatgttt tcaaatcctt gtattcagaa gatgcagaaa gtcgttattc
    20521 tttagaaatt ttagaagata tcgcgcgtgc aactgcaacc atattacgac aagatagtca
    20581 gatgactgtt gaaagtgaag aagaacgatt tgaactaatg attgataata ctataaaaat
    20641 taacggtatt attgaccgta tcgatcgttt atctgatggt tctttgggtg tcgtggacta
    20701 taaatcaagc gctcaaaaat tcgatatcca aaaattttat aacggtctaa gtccacaatt
    20761 agtcacctac attgatgcta tcagtcgtga caaggaagtt gaacaaaaac caccgatttt
    20821 tggagctatg tacttacata tgcaggaacc tagacaagac ttgtctaaaa ttaaaaattt
    20881 agatgattta gtaacaaaaa atcatcaagc tttaacttat aaaggattat tttcagaagc
    20941 cgaaaaagaa ttcttagcaa atgggaaata ccatctcaaa gattcccttt attcagagac
    21001 tgaaattgct attttacaag cacataacca atcgctttac aaaaaagcat ctgagactat
    21061 taagtcaggt aagtttttga tcaatccata tacagaagat gctaaaacag ttgacggaga
    21121 tcagtttaag agtattacgg gattcgaggc ggataggcat atggcacgtg ccagagcgct
    21181 ttataaatta cctgctaagg aaaaacgtca aggtttctta acattaatgc agcaagagga
    21241 ggaaaatgat gacctttaag cctttcttaa atccagaaga tattgctgtt attcagacag
    21301 aagaaaaaaa ttctgataaa aaacaaaagc gaacgcctga acaaattgaa gccatttata
    21361 cttttggtaa caatgtcctg gtatcagctt cggctggttc tggaaagact tttgtaatgg
    21421 ttgagcggat tttagataaa cttttaagag gggttccgat tgactctttg tttatctcaa
    21481 catttacagt aaaagcggca ggtgagctca aagaacgttt agaaaagaaa ataaacgagt
    21541 ctttgaaatc cgcagaatca gatgatttaa aacagtttct cacccagcaa cttgtcggca
    21601 ttcaaacagc tgatattggt actatggatg cttttacgca aaaaatagtt aaccaatatg
    21661 gttatacttt gggcatatca cctatattta ggattttaca agataaaaat gagcaagacg
    21721 tcataaagaa tgaggtctat gctgatttat tttctgacta catgactggg aagaatgcag
    21781 cttcattcat aaaattagtc aaaaatttct caggtaatcg taaagatagc aaagcttttc
    21841 gagaaatggt ttataaagtt tatgctttta gccagtccac cgataacccc aagcgctgga
    21901 tgcaaacagt ttttctaaaa ggtgcacaaa cttacactga ttttgaagct attccagatc
    21961 aagaagtttc aagcctttta aatgtaatgc aaacaactgc taatcaatta cgtgatttaa
    22021 cggatcaaga agattataag caattaactg ctaaaggggt tccgacagca aattacaaaa
    22081 aacaccttaa aataattgaa aatttagtcc attggtctca agactttaat ttgctgtatg
    22141 gaaaaaaagg attgactaat ttggcacgtg atatcactaa tgtgattcct tcaggcaatg
    22201 atgttacagt tgctggcgtt aaatatccta tctttaaaca attgcataat cgtatcgttg
    22261 gacttaagca cttagaagtt atttttaagt atcaaggaga atctcttttc ttgttagaat
    22321 tattgcaatc ctttgtttta gatttctcag aacaatattt acaggagaag attcaagaaa
    22381 atgcttttga attttctgat attgcccatt ttgcaattca aatattggaa gaaaatcatg
    22441 atattaggca actttatcaa gataagtatc atgaagtaat ggttgatgag tatcaggaca
    22501 ataaccatac ccaagagaga atgttagaat tattgtctaa tggacataat cgtttcatgg
    22561 ttggagacat aaaacaatca atttaccgtt ttcgtcaggc tgatccacaa atttttaatg
    22621 ataaatacaa agcttaccaa gacaatccaa gtcaagggaa attaattatt ctgaaagaga
    22681 attttcggag tcaatcagag gttttagaca gtacaaatag tgtgtttaca catcttatgg
    22741 atgaagaagt aggagacatt ctttatgatg aaagtcacca attaaaagct ggtagtccaa
    22801 gacagcaaga aagacatccg aataataaaa cacaagtttt actattagat actgatgaag
    22861 atgatataga tgacagtgat agtcaacaat atgatattag cccagctgaa gcaaagttag
    22921 tcgcaaaaga aattattcga ttgcataaag aagagaatgt tccctttcaa gatatcaccc
    22981 tcttagtttc ttcaaggaca aggaatgatg gtatcttaca aacttttgac cgttatggta
    23041 ttcctttggt aactgacggt ggggagcaaa attacttaaa atcagtcgaa gtgatggtaa
    23101 tgcttgatac tttgcggtca attgataatc ccttaaatga ttatgctcta gttgctcttt
    23161 tgagatctcc catgtttggc tttaatgaag atgatttaac aagaattgct attcaggatg
    23221 tcaaaatggc tttttatcat aaagtgaaat tgtcttatca taaagaaggt catcatagtg
    23281 atttaattac tccagaatta tcttctaaaa tagatcactt tatgaaaaca ttccagactt
    23341 ggcgtgattt tgctaaatgg cattcgcttt atgatttaat atggaaaata tataatgacc
    23401 gtttttatta tgattatgta ggagctctgc ctaaagctga gcaacgtcaa gccaatctat
    23461 atgccttggc acttcgagca aatcagtttg aaaaaacagg tttcaagggt ttatcacgct
    23521 ttattcgaat gattgataaa gttttagaaa atgaaaatga tttggcagat gttgaggtgg
    23581 cattaccgca aaatgctgtt aacctaatga ctatacacaa atcaaagggg cttgagttta
    23641 agtatgtatt tattttaaat attgataaga aattttcaat ggtagacata acttcccctc
    23701 ttattttaag ccgaaatcaa ggaattggta ttaaatatgt tgctgatatg cgacatgaac
    23761 tcgaagaaga gatactgcct gcagtaaagg ttagcatgga aacacttcct taccaactta
    23821 ataaaagaga gttacgctta gctactttat cagagcaaat gcgtttactt tacgtagcta
    23881 tgacacgtgc agagaaaaaa ttatatctgg ttggtaaggc tagtcaaacc aaatgggctg
    23941 atcattatga cttagtatct gaaaataatc atctcccatt agctagtcgt gagacttttg
    24001 tgacctttca agattggtta ctggctgttc atgaaaccta taagaaacaa gagttgtttt
    24061 atgacatcaa cttcgtatca ttagaggaat taacagatca ccacataggg atggttaacc
    24121 ctagtctacc tttcaatccc gataataagg tagagaatcg tcagtctgaa gatatcgtgc
    24181 gtgcgattag cgtgttagaa tctgttgagc agattaatca aacttataaa gcggctattg
    24241 agttaccgac agtgagaaca ccaagtcaag tcaaaaaatt ctatgagcct attctagata
    24301 tagaaggtgt tgatgtgatg gaaactatta ctaaaacatc agtagatttt aagttacctg
    24361 atttttctac aagtaaaaag caagacccag cagctttagg atctgctgta catgaattaa
    24421 tgcaacgcat agagatgtca tctcatgtca aaatggaaga tattcaaaaa gctttgacag
    24481 aagtgaatgc tgaaacaagt gtcaaagcgg ctattcagat tgaaaaaatc aactattttt
    24541 tccaagaaac ctcactcggt aagtatatac aagaagaagt agaacatctg catcgagagg
    24601 caccttttgc catgttaaaa gaagatccgg agagtggtga gaaattcgtt gtacgtggta
    24661 ttattgatgg gtatctacta ttagaaaatc ggataattct atttgattat aagactgata
    24721 aatttgtcaa tcctctagaa ttaaaagaac gctatcaggg acaaatggct ttatatgcag
    24781 aagctttaaa aaaatcttac gagatagaaa agatagacaa atacttaatt cttttaggtg
    24841 gtaaacagct agaagtggta aaaatggatt aaagaaagga aaaaatgata gtcgaacaaa
    24901 aatttggaaa tggttttact tggattaaca ttgaagcaga gcaattgcga acagaaacat
    24961 cggaaattca agcaaaatat ttggatagtg agattattac atatgctctg gatgattatg
    25021 aacgagcctt tatggaatgt tctcatatta aaggaaaaga agtcttgacg attattttta
    25081 atactattga tctaaagcag aaagaaagtt attatgagac cgttccgatg accttttgct
    25141 tatcacacga ccgtttgata acggtgacac ggtctcgaaa tagttatatg ctagaactat
    25201 tgcaaaaata tctagaccgt aatccagatg tctctcctaa aaaattttta tttgcagctt
    25261 taactttgat tacaaaacaa tattttaatg ttgtatcgaa aattgataga gaaaaagata
    25321 ttctcaaccg tcaattgaga gaacaaacaa ccaataagcg cttattagcc atgagtgacc
    25381 tagagacagg atccgtttat cttttgacag ctgccaatca aaatgcattg gtattagagc
    25441 aattagatgt tcatcctagt cagcgattta attctgaagt ggaaaaagaa cagttatcag
    25501 atgctctcat tgaagcgcat cagttagtca gtatgacaca attaaacagt caggtacttt
    25561 cacaattatc atctactttt aataatgtct taaataataa cttgaacgaa aacttaacag
    25621 gtttgaatat catttcgatt aatttagcga ttattgcagc aattactggc ttttttggga
    25681 tgaatattcc attacctctt acagaatcac ggagttcatg gttaattgtt attgctactt
    25741 cggtcttact ttgggttatt attgctcaaa ttttaaaacg agtttttaac ttaagaaaat
    25801 aaaaatattc ggtgcttacg caccgaatat ttttttattt acctaagcag aattggctga
    25861 agagctgagt tattagttca tctggagctg catccccagt gatttctcca agtatttccc
    25921 aagttctagt catatcgact tgtaagagat caacaggcat tcctaattcc aagccctcat
    25981 tgacagcttt taaactatca acggcttttt caattagtga gatatggcga gcatttgata
    26041 gataagtagc gtcatgttct accataccag cgttgtcaaa gaaaatatcg ttaatacgtt
    26101 cttcgatttt atcgatgttt tcattttcta aaaccgaaat tggaataaca tttttaggta
    26161 attcgttaac ttctattttt tgaggaagat ccgttttatt gagtaatacg atacgattac
    26221 tttctttact tagttctaga agggagcggt cttgtaatgt tagtggttcg gaactattga
    26281 gaaccaacaa cacaaggtcg gcttcttcta aagccttctt tgaacgttca acaccaattt
    26341 tttcaacgat atcatctgta tcacggatac cagcagtgtc aactagtttt aaaggaactc
    26401 ctttaatatt gacgtattct tcaataacat cacgagttgt tccttcaata tctgtcacaa
    26461 tcgccttttc ttcacgtaag aggttattta gtaagctgga tttaccgaca tttggacgac
    26521 caatgattgc tgttgaaagt ccttctcgaa gaatttttcc acgtctggca gttcgaagta
    26581 aattttccat aagtgcttga aactcttgtg ttttttcacg catcagtgtt gtagtcatct
    26641 cttcaacgtc atcatattca gggtaatcga tattaacttc aacttgggct agagtattta
    26701 aaatttcttg tcgagtgtta ttgattagtg tctttaaaga accatctaat tgcttaacgg
    26761 caatatccat tgctttatct gttttagctc taataagatc cataacagct tcagcctgag
    26821 tcaagtcaac tcgaccattt aaaaaagcac gtttagtaaa ttctcctggt tcagccattc
    26881 gtgctccatg tcgtaaaatt aactgaagaa tttcgttagt aactgcaatt cctccatgcg
    26941 tattaatttc aataacatct tctcttgtga atgtttttgg agcaagcata acgcctaaca
    27001 taacttcatc caagatttca tttttatctg ggtccacaat atgaccatag tttaaggtat
    27061 gggattggat cgcggataga tcttttccac gataaatttt tgatgctatc tttaaggcat
    27121 ctgtaccaga tatacggaca ataccaatag ctccctcacc gagaggagtt gaaatagctg
    27181 cgatggtatc aaattctttt gtaatagaca taaagtctct ctctttcttg gaaattacta
    27241 acattcattg taacctaaag ccctaaaagg tgcaatcatt tgattaaaag agaagatttc
    27301 cataatggtt ttatttgaag gtatgctata ataaaactat gattatacta caaggaaaca
    27361 aaattgagcg ctcattttca ggtgatgtgc tttttgataa tattaatatt caagttgatc
    27421 aacgtgatcg tattgctctt gttggacgta atggtgctgg caaatcaacg cttcttaaaa
    27481 ttttagttgg tgaagaggct ccgacaaaag gagaaataaa taaaaagcgt gatttgagtt
    27541 tgtcctactt ggcacaagat agtcgttttc aatctgaaaa tacaattttc caagaaatgt
    27601 tacaagtttt tgactcatta agagaagtag agaagcgctt acgtgaattg gaattacaaa
    27661 tggggcaagt ttcaggaagt gacctggaac aattgatgaa aacatacgat attttgtcag
    27721 aagagtttcg tgaaaaaggt ggctttacct atgaatcaga tataaaggct attttaaatg
    27781 gttttaagtt taacagtgat atgtgggaaa tgcctattag tgaattgtct ggcgggcaaa
    27841 atacacgatt agcgcttgca aaaatgctac ttgaaaagcc agaattactt gttttagatg
    27901 aaccaacgaa ccatttagac attgatacga ttgcttggtt agaaaattat cttgtaaatt
    27961 atcaaggagc ccttattatt gtcagccatg accgctattt tttagacaag gtagctacag
    28021 taacttatga tttaacgaca cattctctag accgctatgt aggcaattat tcgaaattta
    28081 tggatcttaa ggcagagaaa atagcgactg aagaaaagaa ttttgaaaaa caacaaaaag
    28141 aaattgctaa gttagaagat tttgtacaac gtaacattgt tagggcttca accactaaac
    28201 gagcacaagc tcgtcgtaaa caattagaaa agatggaacg actcgacaaa ccgaatgtag
    28261 agcaaaaatc agctaatatg acttttcatg ctgggaaagt ctcagggaat gttgttttaa
    28321 cacttgagaa tgcagctatt ggttatgaag gtgtttcttt atcggagcca atcgatttgg
    28381 acgtgaagaa atttgatgct attgcaattg taggtccaaa tggtatcggg aaatcaactt
    28441 taatcaaatc gcttgttggg caaattcctt ttatcaaagg agaagcaaaa ttaggagcaa
    28501 atgtcgaaac tgggtattac gatcaaagtc aaagtaattt aacaaaaacg aatactgttt
    28561 tagatgagtt atgggatgct ttttcaacaa cgccagaagt tgaaattaga aaccgtttag
    28621 gagcttttct tttttcaggt gatgatgtta aaaaatcagt ttcaatgcta tcaggtggtg
    28681 aacgagcgcg tttgctattg gcaaaattat ctatggaaaa taataatttt ctcatattag
    28741 atgagccaac aaaccacttg gatattgata gtaaagaagt cttagaaaat gcactcatcg
    28801 aatttgatgg cacattactt tttgttagtc atgatcgcta ttttattaat cgtgtggcaa
    28861 ctaaggttct cgaaatttct gataagggaa gtactctcta tctaggagat tatgattatt
    28921 acctcactaa gaaagccgaa ctagaggaat tagcacgttt aaatgaagaa gaagtgagtg
    28981 cttcaaaaac agaaatcgac gttacatcag attatgagac acaaaaagca aatcaaaagg
    29041 aatttaggaa aattacccgt cgcgttgtag aaattgaggc gcgtttggaa gttcttgaaa
    29101 atgatgaaaa caatatcaat ggtttaatgt tagaaaccaa tgacatcgga aaactctctg
    29161 atttgcaaaa agagctggaa tcaatccaag aagaacaatt gctattaatg gaagagtggg
    29221 aaaatctaaa tatgagacta gatttgtgag aaaatttcaa aaacaatagt ttgtgaaaaa
    29281 aagtacattt taaattgaga tgccttttac gaaaacaaag aaagcggttc aaattcttgc
    29341 atcttgtgaa aaaaacttat acaataatta agtaataaag atttttttgg aggttagaat
    29401 ggtaactcta tctaaagaac aacacttgga tatgtttcta aaaatgcaac gtattcgtga
    29461 tgttgatatg aaatttaaca aattggttcg tcgtggtttc gtccaaggga tgactcactt
    29521 ctctgttggt gaagaagctg cttcagtagg tgctatccaa gatttaacag attcagatat
    29581 cattttctca aaccaccgtg gacacggaca aactattgca aaaggtattg atattggtgg
    29641 aatgtttgct gaacttgctg gtaaagcaac aggaacatca aaaggtcgtg gaggctcaat
    29701 gcacttagca aaccttgaaa aaggtaacta tggaacaaac ggtatcgtcg gtggaggata
    29761 tgcgcttgca gtaggtgctg cccttacaca gcaatatgaa ggaactgata atattgttat
    29821 cgccttttcg ggtgactcag cgactaacga gggctcattc catgagtcag ttaacttagc
    29881 tgctgtttgg aacttaccag ttatcttctt tatcatcaac aaccgttatg gtatctcaac
    29941 agacatcaca tactcaacta aaattcctca cctttacatg cgtgctgatg cgtacggtat
    30001 tccagggcat tatgttgaag atggtaatga ccttatggct gtttacgaga aaatgcatga
    30061 agttatcaat tacgtgcgtt caggaaatgg accagctatt gtagaggttg aatcataccg
    30121 ttggtttggt cattcaactg ctgatgctgg ggtataccgt actaaagaag aagttgattc
    30181 atggaaagct aaagatccag taaaacgcta ccgtgcatac ttaattgaaa atgaaatcgc
    30241 tactgaagaa gagcttgcag ctattgaagc acaagtgatt aaagaagttg aagaaggggt
    30301 taaattcgct gaagaaagtc cattccctga tatgtctgtt gcatttgaag acgtttttgt
    30361 ggactaaaat aacctaaagt agatgagatt tatctcgttc tattattcaa ttgattaaaa
    30421 aacggatatt cggaggaaat aatgagcgaa actaaagtta tggctttgcg cgaagcgatt
    30481 aatgtcgcta tgagcgaaga aatgcgcaaa gatgaaaaag tatttttaat gggtgaagat
    30541 gtaggtgtat atggaggaga cttcggtaca tctgttggta tgttagaaga gtttggtgct
    30601 aagcgcgttc gcgacacacc aatctcagag gctgcgattg caggttcagc tatcggtgct
    30661 gcacaaacag gtttgcgtcc tatcgttgat ttgacattca tggattttgt aacaatcgca
    30721 atggatgcta tcgttaacca aggtgctaaa acgaattata tgtttggtgg aggtctatca
    30781 actccagtta ctttccgtgt ggcatcaggg tcaggtattg gttcggctgc acaacactca
    30841 caatcattag aagcttggtt aacacacatt ccaggtctaa aagttgttgc acctggtact
    30901 gttaatgagt caaaagcact tttgaaatct tctattcttg acaataaccc agttatcttc
    30961 ttagagccaa aagctctcta tggtaagaaa gaagaagtta atatggaccc tgatttctat
    31021 attccacttg gtaaagggga tattaagcgt gaggggactg acttaacaat tgtttcttat
    31081 ggccgtatgc ttgagcgtgt tatgcaagct gctgaagaag ttgctgagga aggtattaac
    31141 gttgaggttg ttgatccacg tacacttatt ccgcttgata aagaattaat tatcgattct
    31201 gttaagaaaa ctggtaaatt gatcttggtt aacgatgctt ataaaactgg tggtttcact
    31261 ggtgaaattg caactatggt tgctgaaagt gaagcatttg attaccttga tcatcctatc
    31321 gtgcgtcttg caagtgaaga tgttccagta ccgtattcac gtgtacttga acaaggaatc
    31381 ttacctgatg tagcaaaaat taaagatgct atctataaag tagttaacaa aggtaaataa
    31441 ttcataaaac aaagagggac tgaggaatga gtgaaaactt agcctctttc cttgtttata
    31501 ggttttaatt tatatcgtta gatgaaacat atatttgacg atatagttta gtgtgaaagg
    31561 aaaactatgg ctgtcgaaat tattatgcct aaactcgggg ttgatatgca agaaggcgaa
    31621 atccttgagt ggaaaaaaca ggtaggtgat gttgttaatg aaggtgacgt cctacttgaa
    31681 attatgtcag ataaaacaaa tatggagatt gaagcagaag actcaggtgt tttactaaaa
    31741 atcacacatg ggaatggaga tgttgttcct gtaactgaga caattggtta tattggtgct
    31801 gaaggtgaag aagtaacaga agcatcttct tctgaaaata catcagttga agaaaacgcg
    31861 acacaagtaa cttcagaacc agaaaaagtt gaagaaacat cagaaccaag tgtgccagca
    31921 gctacaagtg gtgaaaaagt acgtgccact cctgcagctc gtaaattagc aagagagatg
    31981 tctattgacc ttgcactagt atctggaaca ggggcaaatg gtcgcgttca cagagaagat
    32041 gttgaaaact tcaagggtgc tcaaccacgt attacaccat tggcacgtcg aattgctgaa
    32101 gatcaaggag ttgatatagc agaaatcact ggcagtggta ttcgtggtaa aattgttaaa
    32161 aatgatgttt tggctgctat gtcaccgcaa gcagcagagg ctcctgtaga aactaaagct
    32221 acaccaacaa cagaagaaaa acaattacca gaaggtgttg aagtcattaa gatgtctgca
    32281 atgcgtaagg ctatttctaa aggtatgact aactcttacc tcactgctcc aagctttaca
    32341 cttaactatg acattgatat gactgaaatg atggctcttc gcaagaaatt aattgatcca
    32401 atcatggcta aaacaggctt gaaagtcagc tttactgatt tgattggaat ggccgttgtt
    32461 aagacattga tgaaaccaga gcatcgatac ctaaatgctt cattaattaa cgatgcacaa
    32521 gaaattgagc ttcataattt tgttaatatt ggaatcgcag ttggtcttga tgatggtttg
    32581 attgttcctg ttgttcacaa tgctgatcaa atgtcacttt ctgactttgt tatagcatct
    32641 aaagatgtta ttaagaaaac tcaagaaggt aaattaaaat cagctgaaat gtcaggttca
    32701 acattctcaa ttactaactt aggtatgttt ggtactaaga cgtttaaccc aattatcaat
    32761 caaccgaact cagcaatcct tggagtagga gctactattc cgactccaac agtggttgat
    32821 ggtgaaattg tagctcgacc aattatggca atgtgtttaa caattgacca ccgtattgtt
    32881 gatggaatga atggtgctaa gtttatggtt gatcttaaga acttaatgga aaacccattt
    32941 ggtttactta tttgattccg taggtcttaa tttgatataa taagttattc gaaaaacgaa
    33001 agaggaaata aaaaatggct tttgatgtaa ttatgccaaa acttggggtt gacatgcaag
    33061 aaggcgaaat ccttgagtgg aaaaaaaatg aaggtgacac cgttaatgaa ggtgatgtcc
    33121 ttcttgaaat catgtctgat aaaaccaata tggaaatcga agcagaagac actggtgtac
    33181 ttttaaaaat tgtacatcag gcaggagatg ttgttcccgt cactgaagtg attgcctata
    33241 tcggagaaga aggtgaagaa gtaggtacgt catcaccttc agctgatgca actatcacag
    33301 ctgaagatgg tcaatcagtt tctggacctg ctgctccttc acaagagaca gttgcagcag
    33361 caactcctaa agaagaactt gcagctgatg agtacgatat cgtagtcgta ggtggtgggc
    33421 ctgcaggtta ttacgcagca atccgcggtg ctcaacttgg aggaaaaatt gccattgttg
    33481 agaaaactga atttggcggt acgtgtttga atgttggttg tatccctaca aaaacatatc
    33541 ttaaaaatgc tgaaatccta gatggtttga aagtagcagc tggacgtggt atcaaccttg
    33601 cttcaactaa ctatgcaatt gatatggata agactgttgc ctttaaaaat tctgtagtta
    33661 agacactgac tggtggtgta cgtggtctct tgaaagcgaa caaagttgaa attttcaatg
    33721 gacttggaca agtaaaccct gataagtctg ttgttattgg cgataaagtt atcaaaggtc
    33781 gtaacgtcgt ccttgcgact ggttctaagg tatctcgtat taatattcca ggtattgaat
    33841 caccattagt attaacatca gatgatattc ttgatttacg tgaaattcct aagtcacttg
    33901 ctgttatggg tggtggtgtc gtaggtattg agcttggtct tgtatgggct tcatatggtg
    33961 ttgacgtaac tgttattgaa atggctgatc gcatcattcc agcaatggat aaagaagtat
    34021 cgctagaact tcaaaaaatc cttgctaaga agggaatgaa gattaaaaca tcagttggtg
    34081 tttctgaaat tgttgaagcc aataatcaat taacgcttaa attaaacaac ggtgaagaag
    34141 ttgttgctga taaagctctt ctttcaattg gacgtgttcc acaaatgaat ggtcttgaaa
    34201 atcttgagcc tgaacttgag atggaacgtg gacgtattaa agttaatgct tatcaagaaa
    34261 cttcaattcc aggtatctat gcaccaggtg atgttaatgg aactcgtatg ttagctcatg
    34321 ctgcataccg tatgggtgaa gtagctgctg aaaacgcact cggcggtaat aagcgtaaag
    34381 cacatctaga tttcactcca gcagctgttt atactcatcc tgaagtagca atggtgggga
    34441 tgacagaaga gcaagcacgt gaacaatatg gtgacatatt agttggtaaa aatagcttta
    34501 caggcaatgg tcgtgctatc gcttcaaacg aagcacatgg ttttgtaaaa gtaattgctg
    34561 aacctaagta taaagagatt cttggtgttc acattattgg tccagctgca gcggaattga
    34621 ttaatgaagc ttctacgatt atggaaaatg agttgactgt ctacgatgta gcacaatcta
    34681 tccatggtca cccaacattc tctgaagtta tgtacgaagc tttcttagat gttctcggtg
    34741 aagctattca caaccctcca aaacgcaaat aattttgttt aatatatttg agattgagct
    34801 tttatagctt caatctcttt tcacgtatat attgtctgaa tattataaaa tattagttag
    34861 gaatttaaaa tgaaatatat cgttaacacg tctaatgatc cggcttacaa tgttgcttta
    34921 gaagcttacg cctttcaaaa gttgacagat atcgatgaaa tctttattct ttggattaat
    34981 gaaccggcta tcattattgg acgccatcaa aatacaattc aagaaattaa caaagagttc
    35041 attgataaaa atggcatcca tgttgtccgt cgtttgtcag gaggcggagc agtttatcat
    35101 gacttgaata atttaaacta cactattatt tctaataata cacaagaagg tgcctttgat
    35161 ttccaaactt tttcaaaacc agttattgat actcttgcta aattaggtgt taaagcagaa
    35221 tttactggtc gtaatgattt agaaatcaat ggacaaaaat ttgcaggaaa tgctcaagct
    35281 tattacaaag gtcgtatgat gcatcatggt tgtcttttat ttgatgttga tatgtcagtc
    35341 ttagggcaag cactcaaagt atctaaagac aaaattgaat ctaaagggat aaaatctgta
    35401 cgcgcacgtg ttacaaatat tgttgaccat ctttcagata aaattacagt acaagaattt
    35461 agcgatgcca ttttagctca aatgaaggaa gaatatccag agatggatga atatgttttg
    35521 agtgatgccg agttatcaga aatccaagct atgcgcgata atcaatttgc tacatgggat
    35581 tggacgtatg gtaaagcacc tgaatacacc attgaacgtg gtgtccgcta tccagctgga
    35641 aaaattacga cttatgctaa tgttgaaaat tcaacgataa aatctgtcaa aatctttggt
    35701 gacttctttg gagtgaaacc tgtggatgat attgaaaaaa tgttggaggg tgtccgctac
    35761 gattacaaag atgttttagc agcgcttaaa acagttgata caagtcaata cttctcacgt
    35821 atgacacccg aagagattac aaaagctatt gtagactaaa atcaaaaaat ctagtcaaag
    35881 tcttcgcctt tggctaggtt ttttgtatgg ttaagtgacg ttatagtagt aaaaaaacaa
    35941 cgttattgat aacgctggtt tttatattat tcaaaatctg cttttgattt aacgtctccg
    36001 tattcttcag gaatctctaa agataaaatt tcttcataac tagggaggac aatctcttta
    36061 ccgtatttgt tacggagggc agttgccaca agacggtaag ctagattagc attacgagaa
    36121 aggattggac cgtgaaagta tgacccaaaa acattcttat aatgaacccc ttcagtacca
    36181 tcttctttgt tattcccatt tccataaatg acggttccta aaggtttttc gtcttctgat
    36241 aaaaaggtac gtccttgatg attctcaaag ccataatatg tttcattgaa ttcatcgttg
    36301 tgaatcttga tatcaccaat atagcgatta ttattttggt ttagcgtata gtgtcccata
    36361 acgccaatgc cctctattcg ttctccatta gcttgaatat agtattgtcc taaaagttgg
    36421 aaacctccac aaatagcaag tactacacca ttattttgaa tgaatttgtt aatgtcttct
    36481 ttcttactgg gaagatcgcg tgcaacaatg gcttgttcgt agtcttgtcc accaccaaaa
    36541 aatgccatct gataatagtt gggatcaaat ctatcttcta atgaaacgat atcaaatgtc
    36601 atttgacatc ctagtttttc tcccacatat ttcatcatta agatatttcc gttatcgcca
    36661 taagtattta ataggttacc ataaagatga gctacattaa gagtgtattt ataatctttg
    36721 gtagtaggag attttagtga ggtataagtc attaattcat ctcctttctg atagcgttgt
    36781 ggttagcaag aatttcacga aactccagca tagctgtata tgttgctaaa atataagcat
    36841 gctcagtctc ttgctgctca atcattgtca taatatcttg cagtttatca gcttgtttta
    36901 tgcgtttctc atcataacca gttacacgta agcgacgagc aatttcagag tggcgaacac
    36961 caccagcaaa tatttctggg atattcatcg tgagaatagt ttcaaaatta gcgtcccaaa
    37021 tccaagaagt atcaataccg tcagcataat tggcattcag tagtactgag agagagaacg
    37081 ggtatggagc aagtttaatc atatcaagag cttgactagc tcctaccgga tttttaatca
    37141 gaactaaagt acatttttta tttccaattg taaaggtttc ttgacgacca aaaacggcac
    37201 ggcttttatc aaatccatcc ttaatttgac ttggttctac accaaaatat tcagcaacag
    37261 atactgccgc caaagcattg taaatattat aaaggccacc cacgttgata ttatattgtt
    37321 ggccatcaat aacgaaacct gatgaagtat ttgtcagatg tgttagcctt gttaaggcat
    37381 aatcaaggtt tggacgttca aaatcacaat taggacaagt gtagtcacca aggttggcat
    37441 atgtgttcaa tcgataagtt aaaattgctt gacactttgg acagagaata ccctcagtat
    37501 tatagtgtgc taatcgtggc tcatgtttgt cggtattaaa tccatagaat tgcacagggt
    37561 tagtcacgct tttagaatta aaaagtggac tgtcaccgtt agctaagata gtagcttgcg
    37621 gagcgttagc agctccatct aaaatcattt gataagttgt gtagatttcg ccataacggt
    37681 ccatttgatc acggaaaata ttggtaaaga cgaacaaact aggctttata tactgtgtaa
    37741 tacggggtaa actcgcttcg tctatttcaa ggacagctat ttttttacca gatttacttt
    37801 tcttagctgt taggaaggtc gaaacaatac ctgtaatcat atttgctcca cttgggttgg
    37861 ttaccacctg accgaaggct tcctttaaaa taccaactgt taaagctgtt gttaaggttt
    37921 taccgttagt accagtcaca actacaattt cataatcttt ggcaattgta tctagtatat
    37981 ctttatcgaa tttgagtgct aaactacccg gataagtaga gccacgtccc attttactta
    38041 aaaggtaatg agcagactta ccggcagcca ctcctagtgc tgtatttatt ttcataaaga
    38101 tattttatca taaaaagatg ctttcttgct aaagaaaata ttatgaaaac atggtataat
    38161 gtagacgatg gaaaataaga aatcaaggag ttaagcaaga aactcttcga caggctacca
    38221 gtagagagga agatatggat attttttcag ctattgattc aaagttttgg gcgagcatta
    38281 tggaaaatcc atggatgatc cttattcatc tcatggatat tatcatagta gctgttctga
    38341 tttataaatt tatcaaagcg ctagcaggca caaaaattat gtccttaatt cagggcgtta
    38401 ttttgtttat catcattaga tttgtgtcag aatggatagg gctaacaacg attacatttt
    38461 tgatgaatca gattgttact tatggtgtaa ttgcaggtgt tgtcatcttt gctccagaga
    38521 ttagaacagg tctcgaaaaa tttgggcgaa caccacaact atttacgcaa cgttctcaat
    38581 taagcagtga tgaaaagcta gtagatgccc ttgttaaggc agttgcttat atgagtccgc
    38641 gtaaaatagg agccttgatt tcaattgaac gtacccaaac tttgcaagag tatatagcta
    38701 caggtatccc attagatgca gatatttcca gtgaattgtt aattaatatt ttcattccta
    38761 atacaccact gcatgatggt gctgttattg taaaagataa aaaaattgct acagcatgct
    38821 cgtacttacc tctatcagaa tcatcttcga tttcaaaaga atttggaaca cgccacagag
    38881 cagcgattgg tctatcagag aattcagatg cactaacagt tattgtttct gaagagactg
    38941 gtggtatttc tgttgctttg aaaggtgaat ttttacatga tctttctaaa gatagctttg
    39001 aagcaattct gagaactcag ttgattcaaa atcaagagga aaactcaaaa ttagcttggt
    39061 ataatcaact attgaggagg aagtagacat gaaaaaattt tttacaaata aattttggtt
    39121 aggcgttgtc tcactctttc tggctatttt attattttta acggcaactg caacaagtat
    39181 gaaccatcaa gacaatagta agattgcagg agcgtctgag acatatacac ataccctaac
    39241 agatgtgcca atagacatca aatacgatag tgatgattac tttattagtg gatattctta
    39301 tggggcagat gtctatatgt catcggttaa tcgtgttaag ttagattctg aaataaatga
    39361 ggatacacga aaatttaaag tagtcgccga tttgacaaat atgaaaccag ggactcacaa
    39421 agtcccacta aaggtagtaa atttgccatc tggtgtaaat gctacggttt ctccaacaac
    39481 aattacagta actatgggta aaaagaaaac aaaagagttt ccggtttatg gacatgttaa
    39541 tgataagcaa attaaagctg gttatgcagt cgataagatg tcagttgatg tgtcaaaagt
    39601 taaagttact agtgatgaat ctattataga tcgtattgac catgtcgctg ccaatatccc
    39661 tgatgataaa gttcttgatg atgattttaa caagacagtt actttacaag ctgtaacagc
    39721 agatggtaca gtattagcaa gtattattca cccttctaaa gcaactttat ctgtcaaagt
    39781 aaaaaaattg accaagacag taccgattaa tttgatacca gtaggacaat tcagcgatag
    39841 tatttcaaaa attaactaca agttgagtca agaaaaagca gtaatttcag gaacaaaaga
    39901 ggctttagaa gctatttctg ttattaatgc tgaggtagat atttcagacg taactaaaaa
    39961 tacagaaaag aaaattaatc tttctgcaaa caatgtttca gttgatcctg ctcaagtaac
    40021 agttcaattg acaactacta aaaaataata gataataaaa acgaaaaaaa tggaggtagc
    40081 gtttttcgct agaaaaaaat tatgggaaaa tattttggta cagatggtgt tcgcggtgaa
    40141 gctaatgttg aactgacgcc agaattggct tttaaattag gacgttttgg aggatatgtt
    40201 ctaagtcaac acgaaactga tcgtccgaga gtttttgtag ctcgtgatac tcgtatttct
    40261 ggtgaaatgc tggaatctgc tttgattgct ggtctacttt cagtaggtat tgaggtttat
    40321 aaacttggtg ttttagccac tcctggagtg tcttaccttg tccgtacaga aaaagctagt
    40381 gcaggggtta tgatttcagc aagccacaat ccagcacttg ataatggtat taagttcttt
    40441 ggtagtgatg gttttaaact tgatgatgat cgtgaattag aaattgaagc tttacttgat
    40501 gcgaaggaag acactttacc acgtccatca gcacaaggtt taggtacttt agtagattat
    40561 cctgaaggac ttcggaaata tgagaagttt atggaatcaa ctggtattga tttagaaggt
    40621 atgaaggttg cgttggatac agcaaatgga gcagcaacag cctcagctcg gaatatcttt
    40681 ttagacttga atgctgatat aagtgttatt ggtgatcaac cagatggttt gaatatcaat
    40741 gatggtgttg gttcgacaca tcctgagcaa ttacaaagtt tagttagaga aaatggttcc
    40801 gacatcggtc tagcttttga tggtgatagt gatcgtttga tcgctgttga tgaaaatgga
    40861 gagattgtag atggtgataa aattatgttt atcattggga aatatctctc tgataaaggg
    40921 cagcttgctc agaatacaat cgtaacaacg gttatgtcaa atctcggctt tcataaagcg
    40981 ctcgaccgtg aaggaattca taaagcgatt accgctgtag gagaccgcta cgtggttgaa
    41041 gaaatgcgta aatcaggata taatcttggc ggagagcaat caggccacgt tattatcatg
    41101 gattacaata ctacagggga tggacaatta acagccatcc aattgactaa ggtcatgaaa
    41161 gaaactggta agaaattatc agagttagct agtgaagtga cgatttatcc tcaaaaatta
    41221 gtaaatattc gtgttgaaaa taacatgaaa gataaagcga tggaagttcc tgcaattgcg
    41281 gaaattattg ctaaaatgga agaagagatg gatggcaatg gtcgtattct agtacgtcct
    41341 agtggtactg agcctcttct tagagttatg gcagaagcgc caacaaatga agcggttgat
    41401 tattatgttg atactatcgc agatgtcgtt cgtacagaaa ttggtttaga ttaaaacggc
    41461 gttattctgc tataaagtcg tcaatgttat aaaataataa agagatggaa ttggagtgga
    41521 gcatcaagca tacgcctttc tctctctttt tgatttgaga agggagttta gagaaaatga
    41581 ctaaagaagc agaattatta tgggctaaac acaagtattt agtcttaagt aaatctcaga
    41641 aaatttattt ggatattaga caaactttga aatcaccaaa ttgtaccgtt ttagacgttc
    41701 agtcactcat tgaccaagca gtgcttttag aagaatcacc atcacaagtg actaatgctt
    41761 atatgcatat atggggctat tttaaaaata aggcagagcg tcaagaaaaa gaagaatttt
    41821 taaccttgct ggaaaaatac aggaaaacag gttatcaacg aagaaaactg ttagcttttc
    41881 taaaacagct attagctaaa tacccaaatt cttatttaca aaactcttct attttcgaag
    41941 aagagtaaaa gattgatagt tgaggtatat aaatgagatt atggcatcaa gatattattg
    42001 agttattgcc taggcagcaa cttttaggac agcatagaga gtgttgtgca ttgagaggga
    42061 atgggtgggg gagaaaacat gagactgttg attatgtttt tcgatattca ccttataggt
    42121 tatttgcata tcatcaatta gttatggaag aaatgatgga gcgtgggtat cgtgtatcta
    42181 aagaatggct aattgcagaa tataggggaa tgaagtgccc aagatatgat acacttaatc
    42241 ctgttgactt ggagacacct atctatcccg agcacaatca agactatcta caagaatgtc
    42301 tatggaattt aaaagcaaaa ggaattgacc ttcctataaa taagataaaa tagagattta
    42361 tctctatttt attttggaat aaaaagctat attatcaagt tttagaggag tgttttttta
    42421 agggcaaagt ggtaaaatag aaacatgtta aagaaaccaa cctcagcata tgtacacatt
    42481 ccattttgta cccaaatatg ttattattgt gatttttcaa aagtttttat taaaaatcag
    42541 cctgtagacg cttatcttca ggctcttatc cgtgaatttc ggtcttatga tattacagaa
    42601 ttgcgcacct tatatattgg aggagggaca cctacatcta taagcgctgt gcaactggac
    42661 tatttattga cggagttaag tagagattta aacttaaata ctttagagga gttcactatc
    42721 gaagctaatc caggagattt aaccgttgat aaaatagagg tgcttcaaaa gtcagctgtc
    42781 aatagagttt ctctaggagt tcagaccttt aatgataaac atttaaagcg tattggacgc
    42841 agtcacaatg aggcacaaat ttattctaca attgatgctc ttaaaacagc tggttttcaa
    42901 aatatctcta ttgacctaat ttatgcctta cctggacaaa cgatggatga tgttagaagt
    42961 aatgtagcca aagctctctc tctaaatatt cctcatttga gtttatatag tttaatttta
    43021 gaacaccata ctgtatttat gaataaaatg cgtcgtggga aattacattt gccaacagaa
    43081 gatttagaag ccgagatgtt tgaatatatc atttctgaaa tggaaagaaa tggttttgag
    43141 cattatgaaa tttctaattt tacaaagccg ggttttgaaa gtcgtcataa tttgatgtac
    43201 tgggataatg tagaatatta tggtgttggt gcaggcgctt ctggttatct tgatggtatt
    43261 cgttatcgta atagagggcc aatacaacat tatctaaagg gggtttcaga aggtaatgca
    43321 aggttatctg aagaagtatt atcaaaaaat gaaatgatgg aagaggagct tttcctcggt
    43381 cttcgtaaaa aagagggtgt ttccatcgga aaatttgaac aaaaatttgg aacgtccttt
    43441 gagaaacgtt acggtcagat tgttcaagaa ctccaaagtg atgggctttt gaaagaaaat
    43501 aatggtttta tccagatgac taaaaaaggt ctcttcttag gagatacggt tgccgagaaa
    43561 tttattgtgg agtaaaaaat gggtttatta tatcgagaaa cttacgaagt gcctttttat
    43621 gaatcagaca ctaatcatta tatgaaatta ccgcagttgt tggctttagc gttacaaatt
    43681 tcagccaaac aatctttaaa attaggaata ggcgatgaca tcgtttttaa aagatacgga
    43741 ttggtttggg tcgttacaga ttatattatt gatattgagc gtttacccaa gcatgctgaa
    43801 aagatagtca ttgagactga agccaaagct cataataaat tattgtgtta ccgctatttt
    43861 tatatttatg gtgaagacgg gcaaaaaata ataacgattt catctgcttt tgtcttaatg
    43921 gattttaaaa cacggaagat tcatcctgtt cttgatgata ttacttcaat ttaccaatcc
    43981 caaagaatca aaaaagttat tcgagggccg aaatatcatc ccattggaga tagtaaagtt
    44041 aaacaatacc atgttcgtta ttttgattta gatatgaatg gacatgttaa taatagtaag
    44101 tatctagaat ggatgtatga tgtgttggac ttagattttc taagtagcca cattcctaaa
    44161 aaaattgatt taaaatatat taaggaaatt caatatggaa cggatattaa gtctcattgg
    44221 tatcaagatg gtcttgtgac acgtcatgat attattggtg gcgatgctat tcatgctcaa
    44281 gctaggattg aatggcagga aaaaaaggag gattaaatgg cttataaagg atatcttatt
    44341 gatttagatg gtactattta caaagggaaa agtcgtatcc cagcaggaga gcgttttatc
    44401 gaacgtttgc aagaaaaagg cattccttat atgttagtaa ctaataatac gactcgtaca
    44461 ccagaaagcg ttcaggaaat gcttcgaggc ttcaatgttg aaacaccact tgaaacaatc
    44521 tataccgcaa ctatggctac ggttgactat atgaatgata tgaaccgtgg aaaaacagct
    44581 tacgttattg gtgaagaagg gcttaaaaaa gctattgcag atgcaggtta tgtggaggat
    44641 acaaaaaatc cagcctatgt ggttgttggt ttagattgga atgttactta tgataaattg
    44701 gcaactgcta cgttagcaat tcaaaatggt gctttattta ttggaaccaa tccagatttg
    44761 aatattccta ctgaacgtgg tttattaccc ggtgctggct ccttaaatgc tttattagaa
    44821 gccgcaacgc gtataaaacc agtctttatt ggaaagccaa atgccatcat tatgaataag
    44881 gcacttgaaa ttttaaatat tcctagaaat caagcagtca tggtcggtga taattatttg
    44941 acagatatca tggctggtat taataatgat attgatactt tgttagtaac aacgggtttt
    45001 acaacagttg aagaagtacc agatttacca attcaaccaa gttatgtttt agcaagttta
    45061 gatgaatgga catttaatga aggataagtt attagtagtt ttaacttgga tttggattat
    45121 atcgttagca actctagcta ca
//
LOCUS       DATRMA010000007        44768 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.007, whole genome shotgun sequence.
ACCESSION   DATRMA010000007 DATRMA010000000
VERSION     DATRMA010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 44768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44768
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.007"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            <1..221
                     /locus_tag="VBC28_000354"
     CDS             <1..221
                     /locus_tag="VBC28_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEO6814358.1"
                     /translation="KTGVEVLDRVGTGDAFAAGLIHALLEKETPQRALEIAMATFKYK
                     HTIQGDINIMTRDDIAYLIEKETNDIKR"
     gene            238..2037
                     /gene="uidA"
                     /locus_tag="VBC28_000355"
     CDS             238..2037
                     /gene="uidA"
                     /locus_tag="VBC28_000355"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HEO6814359.1"
                     /translation="MLYPLLTKTRNTYDLGGIWNFKLGEHNPNELLPSDEVMVIPTSF
                     NDLMVSKEKRDYIGDFWYEKVIEVPKVSEDEEMVLRFGSVTHQAKIYVDGVLVGEHKG
                     GFTPFEVLVPECKYNNEKIKVSICANNVLDYTTLPVGNYSEIIQEDGSIKKKVRENFD
                     FFNYAGVHRPLKLMIRPKNHIFDITITSRLSDDLQSADLHFLVETNQKVDEVRISVFD
                     EDNKLVGETKDSRLFLSDVHLWEVLNAYLYTARVEIFVDNQLQDVYEENFGLREIEVT
                     NGQFLLNRKPIYFKGFGKHEDTFINGRGLNEAANLMDLNLLKDMGANSFRTSHYPYSE
                     EMMRLADRMGVLVIDEVPAVGLFQNFNASLDLSPKDNGTWNLMQTKAAHEQAIQELVK
                     RDKNHPSVVMWVVANEPASHEAGAHDYFEPLVKLYKDLDPQKRPVTLVNILMATPDRD
                     QVMDLVDVVCLNRYYGWYVDHGDLTNAEVGIRKELLEWQDKFPDKPIIITEYGADTLP
                     GLHSTWNIPYTEEFQCDFYEMSHRVFDGIPNLVGEQVWNFADFETNLMILRVQGNHKG
                     LFSRNRQPKQVVKEFKKRWMTIPHYHNKKNSVK"
     gene            2066..2737
                     /locus_tag="VBC28_000356"
     CDS             2066..2737
                     /locus_tag="VBC28_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HEO6814360.1"
                     /translation="MARPLVEQTADRLLHLILEREYPVGAKLPNEYELAEDLDVGRST
                     IREAVRSLATRNILEVRQGSGTYISSKKGVSEDPLGFSLIKDTDRLTSDLFELRLLLE
                     PRIAELVAYRITDDQLQLLEKLVGDIEDAVHAGDPKHLLLDVEFHSMLAKYSGNIAMD
                     SLLPVINQSIHLINANYTNRQMKSDSLEAHREIIKAIREKNPVAAHDAMLMHIMSVRR
                     SALKA"
     gene            2854..3471
                     /gene="eda"
                     /locus_tag="VBC28_000357"
     CDS             2854..3471
                     /gene="eda"
                     /locus_tag="VBC28_000357"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEO6814361.1"
                     /translation="MLNQLKNNYFFAVIRGKSSEDALEIAKHAILGGIRNIEVTFSTP
                     EASKVIKQLSDDFKNNKEIIVGAGTVMTTELAKEAIDAGAKFLVSPHFDSDIANLANE
                     NKVYYFPGCATATEIVVARKYKCQIIKLFPGGVVGPGFIKDIHGPIPDVDLMPSGGVS
                     VSNVVEWRKAGAVAVGVGSALSSKVATEGYDSVTKIAKQFVSALD"
     gene            3488..4888
                     /gene="uxaC"
                     /locus_tag="VBC28_000358"
     CDS             3488..4888
                     /gene="uxaC"
                     /locus_tag="VBC28_000358"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HEO6814362.1"
                     /translation="MAFNTETFMLKNQAAIQLYEEVKRQPIFDYHCHLDPKDIFEDHI
                     FDNIVDLWLGGDHYKWRLMRANGISEAEITGPASNLEKFKAFARTLERAYGNPVYHWS
                     AMELKNVFGVNEILTESNAEEIYHRLNHFLKEHKISPRRLIADSKVMFIGTTDHPLDT
                     LEWHKKLAADESFKTVVAPTFRPDEAFIEHRHFVDFITKLGDITQKEITDFSTFIAAM
                     EERIAYFAQNGCRASDISFTEIVFEQTDELELNDLFNKVCEGYIPNQSEISKWQTAVF
                     MELCRLYKKYGFVTQVHFGALRNNHSTIFEKLGADVGVDSLGDQVALTVNMNRLLDSL
                     VKKDSLPKMIWYNLNPAYNIAVANTLANFQANELGVRSYLQFGAGWWFADTKLGMISQ
                     MNALAEQGMLANFIGMLTDSRSFLSYQRHDYFRRILCTYLGEWIEEGEVPEDYQALGS
                     MAKDIAYQNAVNYFKN"
     gene            4906..5952
                     /gene="uxuA"
                     /locus_tag="VBC28_000359"
     CDS             4906..5952
                     /gene="uxuA"
                     /locus_tag="VBC28_000359"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HEO6814363.1"
                     /translation="MEMSFRWYGEDDPVTLENIGQIPTMKGIVTAIYDVPVGEVWSRE
                     RIQQLKEKVEAAGLKISVIESVPVHEDIKLGRPTRDLLIDNYIQTVKNLAAEGIDTIC
                     YNFMPVFDWTRTDLAYQYPDGSTALIFDETVSKKMDPVNGELSLPGWDASYSKEEMKA
                     IMDAYAEIDEEKLWENLTYFIKRIIPEAEAVGVKMAIHPDDPPYSIFGLPRIITGLEA
                     IERFVKLYDSKSNGITLCVGSYASDPQNDVLEISRRAFELDRVNFVHARNIKLGDGKS
                     FKESAHPSEYGSIDMYEVIKLCHEFGFEGAIRPDHGRMIWGETGRPGYGLYDRALGAT
                     YVSGLYEAVIKGSK"
     gene            6076..6915
                     /locus_tag="VBC28_000360"
     CDS             6076..6915
                     /locus_tag="VBC28_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000047575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEO6814364.1"
                     /translation="MSKTITFTNKVVVITGAGGVLCGYMAKEFAKAGAKVALLDLNQE
                     AAQTFADEIVEEGGIAKAYKANVLSKENLEEVHQAVLEDLGPTDILVNGAGGNNPKAT
                     TDNEFHELDLPSETKTFFELDEAGISFVFNLNYLGTLLPTQVFAQDMVGREGANIINI
                     SSMNAFTPLTKIPAYSGAKAAISNFTQWLAVHFSKVGIRCNAIAPGFLVTNQNRSLLF
                     TEDGQPTARAEKILNNTPMGRFGEASELIGGLFFLADEKSSSFVNGVVLPIDGGFAAY
                     SGV"
     gene            7068..7880
                     /locus_tag="VBC28_000361"
     CDS             7068..7880
                     /locus_tag="VBC28_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEO6814365.1"
                     /translation="MVDTNHKIAFCIDSDGCAMDTMTYKHQLFFGPIAADIFGISEQE
                     RTSFLKEWDRVNLYSRTRGVNRFVGLVMGLEFAGVEHIDKLKEWVETTDSLSNNSLEL
                     ALANDFSRDLEKALEWSNEVNRQIKNYEGDSLAFSGVLESLERLSQFGNVYVVSSANK
                     EAVWQEWEEQGLLKYVDDIYCQDRGKKEDVIATLIASGYCRETILMVGDSLGDLAAAE
                     QNGIAFYPVLVGKEVKSWEILREDIGEAFAKGQFEQQRQKESINTFWANLDN"
     gene            7896..9686
                     /locus_tag="VBC28_000362"
     CDS             7896..9686
                     /locus_tag="VBC28_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000149450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HEO6814366.1"
                     /translation="MTHLVDLTKKPFNLNQEAIEWIEKTINEMTLDEKIGQLFFNMGA
                     SRSEEYLTDVLDRYHIAAVRYNRGSSSEIYDQNLILQTKSKLPMLIAANTEAGGDGAV
                     TDGTKVGDEIKVAATNDPKYAYEMGRIAGMEASAVGCNASFSPIVDLTRNWRNPIIAS
                     RNWGANVDQIISLSKEYMKGIMQYNIVPFAKHFPGDGIDERDHHLSFASNPMSKEEWM
                     STFGRIYGELADAGLPGVMAGHIHLPNVEKEMHPERDLDDMLPASLNKTLLDELLRGE
                     LGYNGAIVTDASHMVGMTASMARRDLLPTAIEAGCDLFLFFNDPDEDIQWMKEGYEKG
                     ILTEERLHDALRRTLGLKAKLGLHNYEGRRQELFMPKDKAMALINTLESQKIADEVAD
                     KAVTLVKDKQKDIFPVNPERYRHILLVNVEGYKGGFGAMIAGNKQRASDYMKELLEAR
                     GHEVTVWESTEERIMKLPQEERAAAIANVYAQKQPIANLTEHYDLIINLVDVNAGGTT
                     QRIIWPAAKGTPDQPFYVHEIPSIVISVQHAFALADMPQVGTYINAYDGLPSTISAVV
                     AKLAGESEFTGVSPVDAYCGLIDTHLWRQC"
     gene            complement(9744..10829)
                     /locus_tag="VBC28_000363"
     CDS             complement(9744..10829)
                     /locus_tag="VBC28_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEO6814367.1"
                     /translation="MSKLNRIRHHLHSVQAELAVFSDPVTVNYLTGFFCDPHERQMFL
                     FVYEDRDPILFVPALEVSRAKQSVPFPVFGYIDSENPWQKIASNLPSFSVSKVLAEFD
                     NLNVTKFQGLQTVFDGHFENLTPYIQNMRLIKSRDEIEKMLVAGEFADKAVQVGFDNI
                     SLNNTETDIIAQIEFEMKKQGINKMSFDTMVLTGNNAANPHGIPGTNKIENNALLLFD
                     LGVETLGYTSDMTRTVAVGKPDQFKKDIYHLCLEAHQAAIDFIKPGVLASEVDAAARN
                     VIEKAGYGQYFNHRLGHGLGMDVHEFPSIMAGNDMEIQEGMCFSVEPGIYIPDKVGVR
                     IEDCGYVTKTGFEVFTKTPKELLYFEG"
     gene            11039..12043
                     /gene="ccpA"
                     /locus_tag="VBC28_000364"
     CDS             11039..12043
                     /gene="ccpA"
                     /locus_tag="VBC28_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HEO6814368.1"
                     /translation="MNTDDTITIYDVAREAGVSMATVSRVVNGNKNVKENTRKKVLEV
                     IDRLDYRPNAVARGLASKKTTTVGVVIPNIANSYFSILARGIDDIAAMYKYNIVLASS
                     DEDDDKEVNVVNTLFAKQVDGIIFMGHHLTEKIRAEFSRSRTPIVLAGTVDLEHQLPS
                     VNIDYKAAAVDVIDILAGNHKDIAFVSGPLIDDINGKVRLAGYKEGLKKNGLNFKEGL
                     VFEANYRYAEGFALAQRVINAGATAAYVAEDELAAGLLNGLFEAGKRVPEDFEIITSN
                     DSPIAQYTRPNLTSISQPVYDLGAVSMRMLTKIMHKEELEEKEIVLNHGIVKRGTTNN
                     "
     gene            12175..13641
                     /locus_tag="VBC28_000365"
     CDS             12175..13641
                     /locus_tag="VBC28_000365"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000180581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HEO6814369.1"
                     /translation="MTNELIMQAFEWYLPSDGNHWKKLEESISDLKKLGISKIWLPPA
                     FKGTSSDDVGYGVYDLFDLGEFDQNGTIRTKYGRKEEYLKLIKSLKANGIKPFADIVL
                     NHKANGDHKEKFQVIKVNPENRQEALSEPYEIEGWTGFDFPGRQGEYNDFKWHWYHFT
                     GLDYDAKNNETDIFMIVGDNKGWADDDLIDDENGNFDYLMYNDIDFKHPEVIKNLQDW
                     AKWFIETTGIEGFRLDAVKHIDSYFIQTFINDIRTKIKPDLEVFGEYWKSDQTSMKDY
                     LEATQFQFSLVDVTLHMNFFDASHQNRDFDMRTIFDDSLVIDNPEYAVTFVENHDTQS
                     GQALESRVEDWFKPLAYGLILLRQQGTPCLFYGDYYGIQGEFGQPSFKEVIDKMAELR
                     QNYVFGKQVDYFTHSNCIGWTCLGDEEHNSCLAVVLTNGDQGWKHMEVGEIYAGKTFV
                     DYLGNCEQEVVIGDDGWGDFLVESASISAWVPKIEEAN"
     gene            13686..14684
                     /locus_tag="VBC28_000366"
     CDS             13686..14684
                     /locus_tag="VBC28_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEO6814370.1"
                     /translation="MKVLLYLEAEEYLKKSGIGRAIKHQEKALQIAGIDYTTNPTDDF
                     DLVHMNTYGIRSWLLMSKAKKTGKKVIMHGHSTEEDFRNSFIGSNLVSPLFKWYLCRF
                     YQKADAIITPTDYSKQLIKAYGIKKPIFVLSNGIDLSRYQRSEKKESAFRHYFHLSKD
                     DKVVMGAGLYFMRKGIDQFVEVAAKMPDIRFIWFGETNKWVIPRKVRQIVTKQHPSNV
                     TFAGYIKGDVYEGAMSASDAFFFPSREETEGIVVLEALASHQHVVLRDIPVYHGWVTE
                     DSVELATDVDGFVEKLDKVLSGKSDKIKEGYHVAESRSIERIAHELASVYQKVMEL"
     gene            14686..16020
                     /locus_tag="VBC28_000367"
     CDS             14686..16020
                     /locus_tag="VBC28_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEO6814371.1"
                     /translation="MRIGLFTDTYFPQVSGVSTSIRTLKEGLEKEGHEVYIFTTTDRN
                     VKRFEDPTIIRLPSVPFISFTDRRVVYRGLISAYRIAKDYELDIIHTQTEFSLGLLGK
                     LVAKALRIPVVHTYHTQYEDYVGYIAKGKLIKPSMVKYIMRTYLSDLDGVICPSRIVL
                     NLLDGYGVKIPKQVIPTGIPVENYRREDISEETIKNLRTELGLADNDTMLLSLSRVSF
                     EKNIQAILMHLSAVVDENPHVKLVIVGDGPYLSDLKELVHSLELENSVIFTGMVEHSQ
                     VAIYYKACDFFISASTSETQGLTYIESLASGRPIIAQSNPYLDDVISDKMFGTLYKKE
                     SDLADAILDAIAETPKMTQEAYEQKLYEISAENFSKSVYAFYLDFLISQKASVKEKVS
                     LTIGNKDSHSTLRFVRKAVYLPKKVFTFTGRASKKVVKAPKRRISSIRDFLD"
     gene            16477..18420
                     /gene="thrS"
                     /locus_tag="VBC28_000368"
     CDS             16477..18420
                     /gene="thrS"
                     /locus_tag="VBC28_000368"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEO6814372.1"
                     /translation="MIKITFPDGAIREFESGITTFEIAQSISNSLAKKALAGKFNGQL
                     IDTTRAIEEDGSIEIVTPDHEDALGVLRHSAAHLFAQAAKRLFPDLCLGVGPAIQDGF
                     YYDTDNKSGQISNDDLPRIEEEMKKIVKENHPCIREEISKEEALELFKDDPYKVELIS
                     EHAEDGLTVYRQGEFVDLCRGPHVPSTGRIQVFHLLNVAGAYWRGNSDNAMMQRVYGT
                     AWFDKKDLKAYLKRREEAKERDHRKLGKELDLFMVNPEVGQGLPFWLPNGATIRRELE
                     RYIVDKEIASGYQHVYTPPMASVEFYKTSGHWDHYREDMFPTMDMGDGEEFVLRPMNC
                     PHHIEVYKHHVHSYRELPIRIAELGMMHRYEKSGALTGLQRVREMTLNDAHIFVTPEQ
                     IKDEFLKALNLIAEIYEDFNLTDYRFRLSYRDPEDKHKYYDNDEMWENAQAMLKEAMD
                     DFGLDYFEAEGEAAFYGPKLDIQVKTALGNEETLSTIQLDFLLPERFDLKYIGADGEE
                     HRPIMIHRGGISTMERFTAILIETYKGAFPTWLAPQQVSVIPISNEAHIDYAWEVARV
                     LKDRGIRAEVDDRNEKMQYKIRAAQTQKIPYQLIVGDKEMEEKAVNVRRYGSKATETK
                     SIEEFVESILADIARKSRPDEVK"
     gene            18642..19346
                     /locus_tag="VBC28_000369"
     CDS             18642..19346
                     /locus_tag="VBC28_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO6814373.1"
                     /translation="MRTVLVVQGDDETIELLRSYLEGALYKVVMASDGEEAFSLFQQH
                     QIDLAIIDITLPKIDGYELTRLIRQDSQIPIIMLAAKTTDMDRILGLNIGADDFITKP
                     FNSLEVLARINSQLRRYYEFNSLAKPKNQFIKIGELELDEEHVELTKNGKHIKLTATE
                     FKILHILMSSPGRIYTKTQLYEKINGRYLEGDETTIMVHISNIRDKIEDDSKYPKYIK
                     TLRGVGYKIEKPQYLV"
     gene            19444..20463
                     /locus_tag="VBC28_000370"
     CDS             19444..20463
                     /locus_tag="VBC28_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3114 domain-containing protein"
                     /protein_id="HEO6814374.1"
                     /translation="MAKTALNIPFTVEGIENYYQNIKMILSLADKWSIPKDDLLLFLK
                     GLKAIDDIKVGSPIFKYFWTSLSLQAPLKALEFVLEQAKMPTELSGELSETQYLVAQF
                     SDELAPHDDFWIALSQVIYDSFPGNSLAEDTVLNRKLHQFRYLISSQQAQYVRRYFKD
                     VGMTDRDALVNYLSCLREPDSIAYYESARLHNKRRRNGEIFGFPDDEPVINSKLLISF
                     HTEFIIDDKGNFLNEIDAEVITRNGIINGASFNYAFKNNTRHKELDVDPVKLDPKFRN
                     DMTRGYRSPNLSRRKWFFFKEEDYDCSYFNKKGYYAFGRRSAKQSVDKQVKYLKKAVQ
                     KMRIN"
     gene            20631..21197
                     /locus_tag="VBC28_000371"
     CDS             20631..21197
                     /locus_tag="VBC28_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEO6814375.1"
                     /translation="MIKKNKVFLGVLLVLVVILGGGVLFYQSQFQKTTNQALAIAYKD
                     AKVAKKDVIHQKIDKEFENFRGSYEIEFNTKSAEYSYHVDVKTGQILERDMDNNGFSK
                     STSQSSSSSSQKSHKISQEEAKKIAFKDANIEESEVSNLKIKEEIENGKSVYDIDFVD
                     LKNKNEVDYQIDAETGKIIERSRDHMND"
     gene            complement(21246..21896)
                     /locus_tag="VBC28_000372"
     CDS             complement(21246..21896)
                     /locus_tag="VBC28_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEO6814376.1"
                     /translation="MSSVLTLTKILFLLQGFGLTLYISFISILLSMFFGTLLAIMRNS
                     KNPIWKLIASIYIEFVRNVPNLLWIFIIFLVFQMKSVSAGITSFTIFTSAALAEIIRG
                     GLNGVDKGQTEAGLSQGFTYLQVFIIIIFPQAFRKMLPAIISQFVTVIKDTSLLYSVI
                     AIQEIFGKSQILMGRYFEAGQVFTLYAIITAVYFITNFIISSFSRKLSKRWEQATE"
     gene            complement(21908..22603)
                     /locus_tag="VBC28_000373"
     CDS             complement(21908..22603)
                     /locus_tag="VBC28_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEO6814377.1"
                     /translation="MTLLNISPFAISRWGAFFNHFDLFFKGFLYTLGISFGALLLALI
                     LGILSGGLSTSKSKVGKLISRIYVEVFQNTPLLVQMVFVYYGLAIISNGHVMISAFFT
                     AVLCVGLYHGAYISEVIRSGIEAVPKGQTEAALAQGFTANQTMQLIILPQAVRTILPP
                     MTNQVVNLIKNTSTVAIISGADIMFVAKAWAYDTTNYIPAFAGAAIFYFVICFPLASW
                     ARKQEELNKKTYS"
     gene            complement(22616..23416)
                     /locus_tag="VBC28_000374"
     CDS             complement(22616..23416)
                     /locus_tag="VBC28_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000946176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEO6814378.1"
                     /translation="MLRRKRLTFYLLSCIFIFLLFYPNSTSANQLSEIKKSGVLKVGV
                     KQDVPNFGYYNAETNQYEGMEIDIAKKIAKSLGVKPVFVPTTAQTREPLMDNGQIDIL
                     IATYTITPERKANYNISKAYYHDEIGFLVRKNSHIKTIKELDGKHIGVAQGATTKVNL
                     EKYAKEHKLKFSYAQLGSFPELAISLYANRIDAFSVDKSILSGYLSPHTTILKEGFNT
                     QEYGIATSKQDKVLIPYVNKLLVSWEKDGSLKHIYQKFKLKPAKVKKE"
     gene            complement(23428..24183)
                     /locus_tag="VBC28_000375"
     CDS             complement(23428..24183)
                     /locus_tag="VBC28_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEO6814379.1"
                     /translation="MSLISYKNVNKYYGDYHALRQINLEIEPGQVVVLLGPSGSGKST
                     LIRTMNALESIDDGSLVVNGHELANISSKELVNLRKEVGMVFQHFNLYPHKTVLENIT
                     LAPIKVLKQSKKEAMEIAEKYLKFVNMWERKDSYPSMLSGGQKQRIAIARGLAMHPKL
                     LLFDEPTSALDPETIGDVLSVMQKLANDGMNMVVVTHEMGFAREVADRIIFMADGEIL
                     VDTTDVQDFFDNPREPRAKQFLSNIINHTSTDL"
     gene            24523..25233
                     /locus_tag="VBC28_000376"
     CDS             24523..25233
                     /locus_tag="VBC28_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO6814380.1"
                     /translation="MKKILIVDDEKPISDIIKFNLTKEGYETATAFDGREALVQYAEF
                     QPDLIILDLMLPELDGLEVAKEVRKTSHIPIIMLSAKDSEFDKVIGLEIGADDYVTKP
                     FSNRELLARVKAHLRRTENIETAVAEESAQNASSDITIGELQILPDAFIAKKRGEEIE
                     LTHREFELLHHLATHIGQVMTREHLLETVWGYDYFGDVRTVDVTVRRLREKIEDTPGR
                     PEYILTRRGVGYYMKSYE"
     gene            25226..26575
                     /gene="vicK"
                     /locus_tag="VBC28_000377"
     CDS             25226..26575
                     /gene="vicK"
                     /locus_tag="VBC28_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     VicK"
                     /protein_id="HEO6814381.1"
                     /translation="MNNSAANIRSFELALLFLLVFVAVYFVYLAVRDFKMSKNIRLLN
                     WKVRDLIAGNYSDSILIQGDADLVELGESLNDLSDVFRMAHDNLEQEKNRLASILTYM
                     TDGVLATDRSGKIVMINETAQQQFNLAYDEALSMNIVDMLGSGSPYSFQDLVSKTPEV
                     VLNRRDENGEFVTLRIRFALNRRESGFISGLVAVSHDATEQEKEERERRLFVSNVSHE
                     LRTPLTSVKSYLEALDEGALNEEVAPSFIKVSLDETNRMMRMISDLLSLSRIDNEVTH
                     LDVEMTNFTAFMTSILNRFDQIRNQKTVTGKVYEIVRDYPLKSIWVEIDTDKMTQVID
                     NILNNAVKYSPDGGKITVNLRTTKTQMILSISDQGLGIPKKDLPLIFDRFYRVDKARS
                     RQQGGTGLGLSIAKEIVKQHKGFIWAKSEYGKGSTFTIVLPYDKDAVTYEEWEDVED"
     gene            26579..27388
                     /locus_tag="VBC28_000378"
     CDS             26579..27388
                     /locus_tag="VBC28_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEO6814382.1"
                     /translation="MSEIGFKYSILASGSTGNCFYIETPQKRLLIDAGLTGKKVTSLL
                     AEINRKPEDLDAILVTHEHSDHIKGVGVLARKYHLDIYANEQTWKVMDERNMLGKVDV
                     SQKHVFGRGKTLTFGDLDIESFGVSHDAVDPQFYRMMKDDKSFVMLTDTGYVSDRMAG
                     LIENADGYLIESNHDIEILRSGSYPWTLKQRILSDKGHLSNEDGSETMIRTIGNRTKH
                     IYLGHLSKENNIKELAYMTMENNLMRADFGVGTDFSVHDTSPDSATPLTRI"
     gene            27391..27759
                     /locus_tag="VBC28_000379"
     CDS             27391..27759
                     /locus_tag="VBC28_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HEO6814383.1"
                     /translation="MKKIFYISLGFISLGIGIAGIVLPVVPTTPLVLLSAFCFSRSSE
                     KFDIWLRQTKVYKYYAADFVESRSIAPARKKSMIWQIYILMGISIYFAPLMWLKLGLL
                     IGTIVGTYVLFYVVPDKIDE"
     gene            27935..28621
                     /locus_tag="VBC28_000380"
     CDS             27935..28621
                     /locus_tag="VBC28_000380"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEO6814384.1"
                     /translation="MKELRSKLEKDYGIVFANQELLDTAFTHTSYANEHRLLNISHNE
                     RLEFLGDAVLQLLISQYLFTKYPQKAEGDLSKLRSMIVREESLAGFSRLCGFDHYIKL
                     GKGEEKSGGRNRDTILGDLFEAFLGALLLDKGVEVVHAFVNKVMIPHVEKGTYERVKD
                     YKTSLQELLQSHGDVKIDYQVTNESGPAHAKEFEVTVSVNQENLSQGIGRSKKAAEQD
                     AAKNALATLQ"
     gene            28629..32168
                     /gene="smc"
                     /locus_tag="VBC28_000381"
     CDS             28629..32168
                     /gene="smc"
                     /locus_tag="VBC28_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEO6814385.1"
                     /translation="MFLKEIEMQGFKSFADKTKVEFDQGVTAVVGPNGSGKSNITESL
                     RWALGESSAKSLRGGKMPDVIFAGTENRKPLNYAQVSVTLDNSDHFIENIADEVRVER
                     RIFRNGDSEYLIDGRKVRLRDIHDLFMDTGLGRDSFSIISQGRVEAIFNSKPEERRAI
                     FEEAAGVLKYKTRKKETQSKLEQTQGNLDRLEDIIYELDMQVQPLEKQASIAKRFLVL
                     DEERQGLHLSILIEDILQHQSDLTTVEEKLLTVRKELATYYQQRQSLEDENQSLKQKR
                     HHLSEEIEAKQLALLDVTKLKSDLERQIDLIRLESNQKAEKKEEAGQRLAELEIKAKD
                     CSDQITQKNIELTTLSEKIAQIRSEIVSTESSLERFSTNPDQIIEKLREDFVTLMQEE
                     ADTSNALTALLADIENQKQASQAKSQEIQEVSKNLEVLKSNAKVALERFEAAKKNVRQ
                     LLSHYQDLGQTLQNLEGEYKNQQSILFDHLDEIKSKQARISSLESILKNHSNFYAGVK
                     SVLQAKDQLGGIIGAVSEHLSFDKHYQTALEIALGGSSQHIIVEDESAAKRSIAFLKK
                     NRQGRATFLPLTTIKPRELAQHYLSKLQSSQGFLGIASELVTYDQRLSNIFKNNLGLT
                     AIFDTVDNANVAARQLNYQVRLVTLDGTELRPGGSYSGGANRQNNTVFIKPELDNLKK
                     ELKQAQSKQLIQEKEVATLLEQLKEKQETLAQLKNDGEQARLEEQRADIEYQQLSEKL
                     ADLNKLYNGLQLSSGALEQTTSENEKNRLEKELEQFAIKKEELTTSIAQIKEDKDSIQ
                     EKVNNLTTLLSEAQLEERDLLNEQKFERANCTRLEITLSEIKRDISNLQTLLSHQDSQ
                     LDKEELPRIEKQLLQVNNRRENDEEKLVSLRFELEDCEAALDDLAASLAKEGQKNESL
                     IRQQAQLESQCEQLSQQLMIFSRQLSEDYQMTLDEAKVKANVLEDILMAREQLKSLQA
                     KIKALGPVNIDAIAQFEEVHERLTFLNTQRDDLVHAKNLLLETITDMDDEVKTRFKST
                     FEAIRHSFKETFVQMFGGGSADLILTEGDLLSAGVDISVQPPGKKIQSLNLMSGGEKA
                     LSALALLFAIIRVKTIPFVILDEVEAALDEANVKRFGDYLNRFDKSSQFIVVTHRKGT
                     MSAADSIYGVTMQESGVSKIVSVKLKEAQEMTN"
     gene            32259..33056
                     /locus_tag="VBC28_000382"
     CDS             32259..33056
                     /locus_tag="VBC28_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEO6814386.1"
                     /translation="MIKLVATDMDGTFLDENGTYDKKRLANVLKKFKEQGIVFTAASG
                     RSLLSLEQLFADFRDQMAFIAENGSAAVLFNRLAYEQHLSREQYLDIIDHLSKSPYME
                     NNEYVLSGKDGAYILSDANPDYIEFITHYYDNLQKVSHFEDVDDIIFKVTANFTEETV
                     RQAEEWVNQAIPYATAVTTGFKSIDIILSSVNKRNGLEHLCEQYGIRAEEVLSFGDNI
                     NDLEMLEWSGKAIATENARPEVKEIADCIIGHHNNQAVMAYLESMVD"
     gene            33056..33880
                     /locus_tag="VBC28_000383"
     CDS             33056..33880
                     /locus_tag="VBC28_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEO6814387.1"
                     /translation="MSDIKILALDLDGTLFTTDKKVSEENKVALKAAREKGIKVVITT
                     GRPLKAIGNLLEDLELVSDEDYSITFNGGLVQQNTGKILAKTAMTRQEVEDIHEELYQ
                     VGLPTDILSEGTVYSIANKGHHSQYHLANPLLEFIEVDDLEQVPKDVVYNKIVSVIDA
                     TYLDQQIAKLPDRLKVDYEMFKSRDIILELMPKGVHKAVGLELLTKHLGLDSSQVMAM
                     GDEANDLSMLEWAGLGVAMANGIPEAKAIAKATTICNNDESGVAEAIGKYILSEEN"
     gene            33880..35490
                     /gene="ftsY"
                     /locus_tag="VBC28_000384"
     CDS             33880..35490
                     /gene="ftsY"
                     /locus_tag="VBC28_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEO6814388.1"
                     /translation="MGLFDRLFGHKKKDKEPEIEASESVVLEDEDSVIDKEEGSNFSK
                     ESTLNRTSEVPVAEDDSFLELERDTALSESHQPVTSEIHPLESEDTDEIPVKEDDSFL
                     ELEDRAKTKVADTSEVENVVPDSTTLSDNVSAKSEASFSDKEQLSDSQASDQFSETPL
                     QEEMSSGKTEVQTESEDTSAADAFLADYYAKRKAIEKEISSNSLSTDESEFSEAQEVL
                     SQSQADTIKAESQEEKYNRSLKKTRTGFSARLNAFLSNFRRVDEEFFEELEEMLILSD
                     VGVNVATQLTEDLRYEAKLENAKKSEDLKRVIVEKLVEIYEKDGIYNEAINFQEGLTV
                     MLFVGVNGVGKTTSIGKLAHQYKSQGKKVMLVAADTFRAGAVAQLVEWGRRVDVPVVT
                     GEEKADPASVVFDGMEKAVAQGVDVLLIDTAGRLQNKENLMAELEKIGRIIKRVVPDA
                     PHETLLALDASTGQNALSQAKEFSKITPLTGLILTKIDGTAKGGVVLAIRQELDIPVK
                     FIGFGEKIDDIGEFNSEDFMRGLLEGIL"
     gene            complement(35527..36339)
                     /locus_tag="VBC28_000385"
     CDS             complement(35527..36339)
                     /locus_tag="VBC28_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000617812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HEO6814389.1"
                     /translation="MIRFLILAGYFELSMYLKLSGKLNQYINTHYTYLAYISMVLSFI
                     LAIVQLIIWVKNMKMHSHLHGKIAKSTSPMILVFPVLVGLLVPTVSLDSTTVSAKGYN
                     FPLAAGSTGTVSQDGTRVQYLKPDTSTYFTSSAYEKEMQKELKKYKGSGTLTITTENY
                     MEVMELIYLYPEQFMDRQIQYTGFVYNEPKHEGYQFIFRFGIIHCIADSGVYGLLTTG
                     NQKSYPDNTWVTVRGTIKSEYNQLLQQNLPVLHIEESRQVSKANNPYVYRVF"
     gene            complement(36339..37241)
                     /locus_tag="VBC28_000386"
     CDS             complement(36339..37241)
                     /locus_tag="VBC28_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEO6814390.1"
                     /translation="MDIFNQLPDSVLQWFAIFISIIIEALPFVLLGTILSGIIEVFIT
                     PDIVNKFLPKNKFLRVLFGTFVGFVFPSCECGIIPIINRFLEKKVPSYTAVPFLATAP
                     IINPIVLFATYSAFGNSIRFLILRFVGATIVAIALGVMLAFLVDDNILKEDAKPTHFH
                     DYSDKKWYQKIFLALAHAIDEFFDTGRYLVFGTLIASAMQIYLPTRVLTTIGHSPITA
                     ILVMMLLAFILSLCSEADAFIGASLLSTFGIAPVMAFLLIGPMIDIKNLMMMVNSFKT
                     RFIVQFISVSSLIIIIYCLFVGVI"
     gene            complement(37247..37375)
                     /locus_tag="VBC28_000387"
     CDS             complement(37247..37375)
                     /locus_tag="VBC28_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6814391.1"
                     /translation="MAITHKRQDDLESMFASFAKVPKPKKVDSDSKPEQKDDSQKD"
     gene            37508..38551
                     /locus_tag="VBC28_000388"
     CDS             37508..38551
                     /locus_tag="VBC28_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEO6814392.1"
                     /translation="MIELGITTFGETTILEETNQSYSHPERIRQLVAEIELADQVGLD
                     VYGIGEHHREDFAVSAPEIVLAAGAVRTNNIRLSSAVTILSSNDPIRVYQQFSTIDAL
                     SNGRAEIMAGRGSFIESFPLFGYDLADYDDLFNEKMDMLLAINLATNLDWKGHLTQTV
                     NERPIYPRALQRQLPIWVATGGNVDSTIRIAEQGLPIVYATIGGNPKAFRQLVHIYKE
                     VGSRNGHKPEQLKVAAHSWGWIEEDNQTAIDRYFFPTKQTVDNIAKGRPHWSEMTKEQ
                     YLRSVGPEGAIFVGSPEVVAHKIIGLVEALELDRFMLHLPVGSMPHKDVLNAIKLYGK
                     EVAPIVRKYFEKN"
     gene            38656..40818
                     /locus_tag="VBC28_000389"
     CDS             38656..40818
                     /locus_tag="VBC28_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HEO6814393.1"
                     /translation="MENLNITQIAIDLGIKASQIEKVLELTDEGNTIPFIARYRKEMT
                     GNLDEVQIKSIIDLDKSMTALSDRKTTVLAKIEEQGKLTQELKKAIEEATKLADVEEL
                     YLPYKEKRRTKATIAREAGLFPLARLILQNKDNLEEEAQNYLTDGFETTTKALSGAVD
                     ILIEAFSEDNKLRSWTYNEIWNYSSITAVVKDESLDEKQVFKIYYDFSEKISKLHGYQ
                     VLALNRGEKMGVLKVNFEHNLEKMFRFFAVRFKETSQYIDDLIVQTVKKKIVPAMERR
                     IRTELSEGAEDGAISLFSENLRNLLLVSPLKGKMVLGFDPAFRTGAKLAVVDQTGKLM
                     TTQVIYPVPPANQAKIEQSKIELAKLIKEFNIEIIAIGNGTASRESEAFVAEVLQDFP
                     DVSYVIVNESGASVYSASELARHEFPDLTVEKRSAISIARRLQDPLAELVKIDPKSIG
                     VGQYQHDVSQKKLAENLDFVVETVVNQVGVNVNTASPALLAHVSGLNKTISENIVKYR
                     EENGQIKSRAEIKKVPRLGAKAFEQAAGFLRIPNAKNFLDNTGVHPESYEAVKKLLDQ
                     LTIKELDDLAKEKLQNLDLIATAESIGVGQETLKDIIEDLLKPGRDLRDDFEAPVLRH
                     DVLDVSDLKVGQELQGTVRNVVDFGAFVDIGVHEDGLIHQSRLIKRKRDKKTRKMPPL
                     QHPSKYLSVGDIVTVWVVEVDAERSRIGLSLIKPDGLD"
     gene            40781..41239
                     /locus_tag="VBC28_000390"
     CDS             40781..41239
                     /locus_tag="VBC28_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HEO6814394.1"
                     /translation="MDLVLSSQTDLTNYVKKVSIEDFGCQFLHTAHWNNRLRTTGGRF
                     FPNDRHLDFNPKIYREFGIDIFRKIVRHELCHYHLYIQGKGYQHRDKAFKELLEKVDG
                     LRYTPRVTAVKVNYHHYSCQSCGQVYRRRRRVNLRKFACGYCHGRLIESF"
     gene            41320..41583
                     /locus_tag="VBC28_000391"
     CDS             41320..41583
                     /locus_tag="VBC28_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEO6814395.1"
                     /translation="MKSSFYKQRKGKLVCGVVAGLADKYNWDLALSRVLIALILYFTK
                     FGLLLYILLAVFLPYKEDIIETRRQGPRRRKDAEPVDDDGWFW"
     gene            41811..42746
                     /locus_tag="VBC28_000392"
     CDS             41811..42746
                     /locus_tag="VBC28_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HEO6814396.1"
                     /translation="MAVTVQMLVDRLKLNVIYGDEHLLSKRITTADISRPGLEMTGYF
                     DYYAPERLQLVGMKEWSYLMAMTGHNRYQVLREMFQKETPAIVVARDLEIPEEMYEAA
                     KDTGIAILQSKAPTSRLSGEVSWYLDSCLAERTSVHGVLMDIYGMGVLIQGDSGIGKS
                     ETGLELVKRGHRLVADDRVDVYAKDEETLWGEPAEILRHLLEIRGVGIIDIMSLYGAS
                     AVKDSSQVQLAIYLENFETGKVFDRLGNGNEEIELSGVKVPRIRIPVKTGRNVSVVIE
                     AAAMNHRAKQMGFDATQTFEDRLTHLISQNEVNDD"
     gene            42739..43512
                     /locus_tag="VBC28_000393"
     CDS             42739..43512
                     /locus_tag="VBC28_000393"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEO6814397.1"
                     /translation="MINPVAIRLGPFSIRWYAICIVSGMLLAVYLAMKEAPRKNIKSD
                     DILDFILMAFPLSIVGARIYYVIFEWAYYSKHPVEIIAIWNGGIAIYGGLITGAILLV
                     IFSYRRLINPIDFLDIAAPGVMIAQAIGRWGNFINQEAYGRAVKNLNYVPNFIKNQMY
                     IDGAYRVPTFLYESLWNFLGFVIIMSIRHRPRTLKQGEVACFYLVWYGCGRFIIEGMR
                     TDSLYLAGLRVSQWLSVILVIIGIVMIIYRRREQHISYY"
     gene            43527..43925
                     /locus_tag="VBC28_000394"
     CDS             43527..43925
                     /locus_tag="VBC28_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HEO6814398.1"
                     /translation="MIEIAVLIIAIAFVVLVLGILFVLKKVSETIEETKQTIKVLTSD
                     VNVTLYQTNEILAKANVLVDDVNGKVSTIDPLFVAIADLSESVSDLNLQARHIGQKAS
                     SATSSVTKAGSALAIGKAASKIFRKKGDKK"
     gene            43922..44353
                     /locus_tag="VBC28_000395"
     CDS             43922..44353
                     /locus_tag="VBC28_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEO6814399.1"
                     /translation="MSKLFKTLVISAASGAAAAYFLTTKKGKELRKNAEKFYGEYKEN
                     PEEYHQIAKDKASEYSNLAVDTFKDYKGKFESGELTTEDIVSAVKEKSGEVVDFANDF
                     VNQAKSKFSDEDTAKKEDKAPETKVEDIVIDYKENTEDKEK"
     gene            complement(44394..44669)
                     /locus_tag="VBC28_000396"
     CDS             complement(44394..44669)
                     /locus_tag="VBC28_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3270 domain-containing protein"
                     /protein_id="HEO6814400.1"
                     /translation="MSSPLKQEQTHESKTEQAQPKFQVFQEFDHSAKLRELVFFARIA
                     LFSITTVLISFLLLALSLTPVWSFLLAMLASLAVTSAVSSFIWSLRQ"
BASE COUNT        14923 a         6810 c         9182 g        13853 t
ORIGIN      
        1 ataaaactgg cgttgaagta ttagatcgtg tggggacagg agatgccttt gctgcaggtt
       61 taattcatgc gcttctggaa aaagaaactc ctcaaagggc cttagaaata gctatggcta
      121 catttaaata caaacatacc attcaaggag atatcaatat catgacacga gatgatattg
      181 cttacctcat tgaaaaagaa acaaatgata ttaaacgata gtgtaaaagg agagtgtatg
      241 ttatatccat tattgacaaa aacaagaaat acttatgatt taggcggtat ttggaacttt
      301 aaattaggag aacataatcc aaatgaatta ctaccttcag atgaagtcat ggttatcccg
      361 acttcattta atgatttgat ggtaagtaaa gaaaaacgtg attatatagg tgatttttgg
      421 tatgaaaaag tcattgaagt acctaaagta tcagaggatg aagagatggt gctgcgtttt
      481 ggctcagtga cacatcaagc taaaatttat gttgacggtg tattggtagg agagcataag
      541 ggaggtttta ctccttttga agttcttgtt cctgaatgta agtataataa tgagaaaatt
      601 aaggtatcaa tttgtgctaa taacgtttta gactatacca cgcttccggt cggaaattat
      661 agtgaaatca ttcaagaaga tggtagcatt aagaagaaag tacgtgagaa ttttgatttc
      721 ttcaattatg caggtgtgca tcgtcctctt aagttgatga tacgtcctaa aaatcatatt
      781 tttgatatta caattacaag tcgtctatca gatgatttac agtcagcaga tttacatttt
      841 ttagtggaga ctaatcaaaa ggttgacgaa gttagaatta gcgtttttga tgaagataac
      901 aaactagtgg gtgaaacgaa ggatagcaga ttatttctta gtgatgtcca tctttgggaa
      961 gttttaaatg cctatctgta cacagcgcgt gttgaaattt ttgttgataa tcaactccaa
     1021 gatgtctatg aggaaaattt tggtcttaga gaaatagaag tgacaaatgg tcaattccta
     1081 ttgaatcgca aacctattta ttttaaagga tttggtaaac atgaagatac gttcattaat
     1141 ggcagaggtt tgaatgaagc tgctaactta atggatttga atcttttgaa ggatatgggg
     1201 gcaaactctt ttagaacatc ccattatcct tattctgaag aaatgatgcg tttagcagat
     1261 cgaatgggag tgttagtgat agatgaggtt ccagcagtag ggttatttca aaattttaac
     1321 gcttccttag atttgtcacc aaaagataat ggtacgtgga atttgatgca aacaaaagcg
     1381 gcgcatgaac aagctatcca agaattagtg aagcgtgata aaaatcatcc tagcgtcgtg
     1441 atgtgggtag tcgctaacga accggctagt catgaagcgg gagcacatga ttattttgag
     1501 ccattagtaa aactttataa agatttagac cctcaaaaac gtcctgtcac cttggttaat
     1561 attttaatgg caaccccaga tagagaccaa gtgatggacc tggttgatgt tgtctgcctt
     1621 aatcgttact atggttggta cgttgaccac ggtgatttaa caaatgcaga agtaggtata
     1681 agaaaagagt tattagaatg gcaagataaa tttcctgaca aaccaattat cataacggag
     1741 tatggcgctg atacgttacc gggattacat tctacttgga atattccata tacagaagaa
     1801 tttcaatgtg atttttatga aatgagtcat cgtgtttttg atggtattcc taatttagtt
     1861 ggtgagcaag tctggaattt tgcagacttt gaaactaatc tgatgatact tcgtgtacag
     1921 gggaatcaca aaggtctctt ttcaaggaat cgccagccga aacaagtcgt caaagaattt
     1981 aaaaaacgct ggatgactat tcctcattac cataataaaa aaaatagtgt aaaatagggg
     2041 tagttatttt agaagaggtg atgatatggc tagaccctta gtagaacaaa cagcagatcg
     2101 tctattgcat ttaattttag aaagagaata ccctgttggt gctaagctcc ccaatgaata
     2161 tgaattagca gaagatctag atgttggtcg cagcacaatc cgagaagcag tcagaagttt
     2221 agcaacgcgt aatattttag aagtcagaca gggctctgga acatatatta gttctaaaaa
     2281 gggtgtttct gaggatccat tagggttctc tctaataaag gacacagata ggttgacatc
     2341 agatctcttt gaactacgcc ttttattgga acctcgaata gcagagctgg tagcttatag
     2401 aattacagat gatcagttac aattactcga aaagcttgtt ggagatattg aagatgctgt
     2461 tcacgcaggc gatccgaaac atttactttt agacgtggaa tttcattcca tgctagctaa
     2521 atacagtggt aatattgcaa tggatagcct tttgccagtc atcaaccagt caattcatct
     2581 gataaatgcg aattatacaa atcgtcagat gaagtcggat agtttagaag cacatagaga
     2641 aattatcaaa gcaatccgag aaaaaaatcc agtagctgca catgatgcta tgcttatgca
     2701 tatcatgagt gttagacgtt cagcattgaa agcttaatat aagaagaaaa atagctattc
     2761 ttgaatagct atttttttag tattcttctt gacaattcat cagatgactt atataatata
     2821 gttaaagata acggttacat tttgggaggt attatgttaa atcaattgaa aaacaactat
     2881 ttctttgctg ttattcgagg gaaatcatct gaggatgcgc tcgaaatcgc aaagcatgct
     2941 attttgggtg gtattcggaa tattgaagta actttttcga ctcctgaagc aagcaaagtc
     3001 atcaaacaat tatcagatga ctttaaaaat aataaagaaa ttattgtggg ggccggaacg
     3061 gtcatgacaa ctgaacttgc taaagaggct attgatgctg gagctaaatt tttggttagc
     3121 ccacactttg attcagatat tgctaattta gctaatgaaa acaaggttta ttatttccca
     3181 ggctgtgcaa cggcaacaga aatcgtggta gctagaaagt ataaatgtca gattataaag
     3241 ctttttccag gtggagttgt gggtccaggt tttatcaaag atattcatgg tcctattcca
     3301 gatgttgatt taatgccatc aggtggcgtc tctgtttcaa atgtagttga atggagaaaa
     3361 gcaggagcag ttgcggttgg tgttggctca gcattatcta gtaaagtagc cacagaaggc
     3421 tatgatagtg ttactaaaat agctaaacag tttgtttctg cacttgatta gtgagtaaga
     3481 gaagaatatg gcgtttaata ctgaaacatt tatgttaaaa aatcaagcag ctatacagct
     3541 gtatgaagag gtcaagagac aacctatttt tgattatcat tgccacttag atccaaagga
     3601 catttttgaa gatcatattt ttgataatat tgtggatttg tggcttggtg gtgaccacta
     3661 taaatggcgt cttatgaggg ctaatggtat ttctgaagca gaaattacag gtccagcatc
     3721 caaccttgag aaatttaaag catttgctag aacactagaa agagcatacg gtaatcctgt
     3781 ttatcattgg tctgctatgg aattgaaaaa tgtctttggt gttaatgaga ttttgacaga
     3841 atcaaatgct gaggaaattt atcatcgttt aaatcatttc ttgaaagagc ataaaataag
     3901 tcctagacgt ttaattgcag atagcaaagt tatgtttatt ggtactacag accaccctct
     3961 tgatactctc gagtggcata aaaagttggc tgctgatgag agttttaaaa cagttgttgc
     4021 tccgacattt aggccagatg aagcttttat tgaacatcga cattttgttg attttattac
     4081 taaactgggt gacattactc aaaaagagat tacagatttt tcaacattta tagcagcaat
     4141 ggaagaacgc attgcgtatt ttgctcaaaa tggttgtagg gctagcgata tcagttttac
     4201 ggaaatcgtc tttgaacaaa ctgatgaatt ggagttgaac gatttattta ataaagtttg
     4261 tgaaggctat atcccaaatc aatcagaaat cagcaaatgg caaacagctg tttttatgga
     4321 actttgccga ttatataaaa aatatggttt tgtaacacag gtgcattttg gagctttgag
     4381 aaataatcat tctactattt ttgaaaaatt gggagctgac gtgggtgtgg attcacttgg
     4441 tgaccaagtg gctttaactg ttaatatgaa ccgtttattg gattccttgg ttaaaaaaga
     4501 tagtctgcct aaaatgattt ggtataatct caatccagct tacaatatag cagtagctaa
     4561 cactttggca aattttcaag ctaacgaact tggagtgaga tcttaccttc aatttggtgc
     4621 aggttggtgg tttgcagata ctaaattagg gatgattagt caaatgaatg ccttagcaga
     4681 gcaaggcatg ttggcgaatt ttattggtat gttaacagat tctagaagtt ttctatctta
     4741 tcaacgtcat gactattttc gacgtatttt atgtacctac ttaggagaat ggattgaaga
     4801 aggagaagtt ccagaagatt atcaagcttt aggaagtatg gctaaagaca tcgcttatca
     4861 aaatgcagtt aattatttta agaactaaaa aggaagagtg aaatcatgga aatgtcattt
     4921 aggtggtatg gtgaagacga ccccgttact ttagaaaata ttggtcaaat tccaacaatg
     4981 aaagggattg ttacagcgat atacgatgta ccagtaggag aagtttggtc acgtgagcgc
     5041 attcaacagc tgaaagaaaa ggtagaggca gcagggctta aaatttcggt tatcgaatca
     5101 gtacctgttc atgaagatat caaattggga cgtccaacac gtgatctctt gattgataat
     5161 tatattcaaa ccgtcaaaaa tttggctgcg gaaggtattg atactatttg ttataatttt
     5221 atgccagtat ttgactggac acgcactgat ttagcatatc aatatccaga tggttccaca
     5281 gctttgatat ttgatgagac ggtttctaaa aaaatggatc cagtaaacgg tgaattatct
     5341 ttgccaggat gggatgccag ctatagtaaa gaagaaatga aggctatcat ggatgcctat
     5401 gctgaaattg atgaggaaaa actttgggaa aatctcactt attttattaa acgtattatt
     5461 cctgaagcgg aagccgtagg cgttaaaatg gccattcatc cggatgaccc accatattct
     5521 atttttggtt tacctcgcat tattactggg ttagaggcta ttgagcgttt tgtgaaattg
     5581 tacgatagta aaagtaatgg catcacttta tgtgtgggtt cttacgcttc tgatccccag
     5641 aatgatgttt tagagatttc gcgacgtgct tttgagttag atcgtgtaaa ctttgttcat
     5701 gctcgcaata ttaaattagg tgatggaaag tcatttaaag aatcggctca tccatcagaa
     5761 tacgggtcaa tagacatgta tgaagttatt aagttatgcc atgaatttgg gtttgagggt
     5821 gctattcgac ctgaccacgg gcgtatgatt tggggagaaa caggtcgtcc aggatatggc
     5881 ctatacgata gagctttagg agccacttac gtttctggtt tatacgaagc tgtgattaaa
     5941 ggttcaaaat aaggcgatgc ttccaaattt ctcctgttcc aatctcctga aagagaaatt
     6001 tggaaagtta ttatcataat atgattgtac ttagctaaga gtagaatact tatagaagtt
     6061 aatgagagga ataagatgtc aaaaacaatt acatttacca ataaagttgt cgtcataaca
     6121 ggtgcaggcg gtgttctctg tggttatatg gctaaagaat ttgcgaaagc aggcgctaaa
     6181 gtggcattat tagatttgaa tcaagaagct gctcaaacat ttgctgatga aattgtagaa
     6241 gaaggtggta tcgctaaggc ttataaagct aacgttttgt ctaaagaaaa ccttgaagaa
     6301 gtgcatcaag ccgttttaga ggatcttgga ccaactgata ttttagttaa tggtgcagga
     6361 ggcaacaatc ctaaagctac aactgataat gaatttcacg aattagactt accttcagaa
     6421 acaaaaacct tctttgagtt agatgaagct ggtattagct ttgtttttaa cttaaattat
     6481 ttaggaacat tattgccaac gcaagtattt gcccaagata tggttggacg tgaaggagcg
     6541 aatattatta atatctcttc aatgaatgct tttacccctc tgaccaaaat accagcttat
     6601 tctggagcta aagcagctat tagtaacttc acacaatggc tagctgttca tttctcaaaa
     6661 gttggtatcc gttgtaatgc tattgcacct ggtttcttgg taacaaatca gaaccgaagc
     6721 ttgttattta ctgaggacgg tcagccaact gcgcgtgctg agaaaattct taataataca
     6781 ccaatgggaa gatttggaga agcaagtgag ctgattggcg gattgttctt tttagcagat
     6841 gaaaaatcat caagttttgt taatggcgtt gtccttccga ttgatggtgg atttgctgct
     6901 tactctggtg tttaatttac aagataatag ccacttaaca cataagagat tattgagttg
     6961 agtggttttt taaggaaatt tgtttaatga tcttaccact aaatgatggg taggttgcta
     7021 attggaaaat cctcataagt tagataaatt tagaaactgg agataatatg gtagatacaa
     7081 atcacaaaat cgctttttgc atagattctg atggttgcgc aatggataca atgacatata
     7141 agcatcaact cttttttggt ccaattgctg cagatatatt tgggatatca gaacaagaaa
     7201 gaacaagttt tttaaaagag tgggatagag tcaatcttta ttcaagaact cgtggagtta
     7261 atcggtttgt gggattggtt atgggccttg agtttgccgg agtagaacat attgataaat
     7321 taaaagaatg ggtcgaaaca acagactcat tgtccaataa ttccttagag ttagcgcttg
     7381 ccaacgattt tagtagagat ttagagaagg ctttagaatg gtccaatgaa gtgaatcgcc
     7441 aaatcaaaaa ttatgaggga gattctttag cgttttcagg tgtgttagaa agtttagaac
     7501 gattaagtca atttggtaat gtttatgtcg tgagttctgc taacaaggaa gctgtttggc
     7561 aagagtggga agaacaggga ttgctaaagt atgttgatga tatctattgt caagatagag
     7621 gtaaaaaaga agatgttatc gccactttaa ttgcaagtgg ttactgccgt gaaactattt
     7681 tgatggtagg agattccctg ggtgatctgg ctgcagcaga acaaaatggt attgcatttt
     7741 acccagtact tgttggtaag gaagtcaaat catgggaaat tcttagagaa gatattggtg
     7801 aagcttttgc aaaaggtcaa ttcgaacagc aaaggcaaaa agaatctatt aatacctttt
     7861 gggctaattt agataactaa tagaaaggat aggctatgac acatttagtt gacttaacaa
     7921 agaaaccctt caatctaaat caagaagcaa ttgagtggat tgaaaaaaca attaatgaga
     7981 tgacattgga tgagaaaatt gggcaacttt ttttcaatat gggagctagt cgttcagaag
     8041 aatatttaac agatgttttg gatcggtatc atattgctgc tgttcgctat aatagaggta
     8101 gttcaagtga aatctatgat cagaatttaa ttttacagac aaagtcgaaa ttgccgatgt
     8161 tgattgctgc aaatacagaa gctggtggag acggtgctgt cacggatgga actaaagttg
     8221 gcgatgaaat aaaagtggct gctacaaatg atccgaaata tgcttatgaa atgggaagaa
     8281 tcgcagggat ggaggcatct gctgtgggtt gtaatgcttc attttctcca attgtagatt
     8341 tgacacgtaa ttggcgtaat cctatcatag ctagtcgaaa ttggggtgct aacgttgatc
     8401 aaattatttc actttcaaaa gagtatatga aaggaatcat gcaatataat attgtacctt
     8461 tcgctaaaca ttttccaggg gatggcattg atgagcgtga tcatcatttg tcatttgctt
     8521 ccaatcctat gagtaaagag gagtggatgt cgacatttgg ccgcatttat ggtgaattgg
     8581 ctgacgcagg tttaccagga gtcatggcag gtcacataca tttaccaaac gttgaaaaag
     8641 aaatgcatcc agagcgagac cttgatgata tgttaccagc ttcattgaat aaaacacttt
     8701 tagatgagtt attgagagga gagttaggtt ataatggagc tattgtaacg gacgcttctc
     8761 atatggttgg aatgactgct tcaatggcaa ggagagattt gttgccaacg gcgattgaag
     8821 caggttgtga tttattctta ttctttaatg accctgatga agacattcaa tggatgaaag
     8881 aaggttacga aaaaggtatt ctaacagagg aacgtctcca tgacgcttta cgtcgtactc
     8941 tcggtctaaa agcgaaatta ggcttgcata actatgaggg acgtcgccaa gaactcttta
     9001 tgcctaaaga taaggctatg gctttaatca acacattaga gtcgcaaaaa atagctgatg
     9061 aagttgctga taaagcagta actctggtaa aagataaaca aaaagatatc tttccagtaa
     9121 atccagaacg ttatcgtcat atcttacttg ttaacgttga aggatataag ggaggttttg
     9181 gtgcgatgat tgctggaaac aagcaacgtg catcggatta catgaaagag ttacttgagg
     9241 cacgtggcca tgaagtaact gtctgggagt ctaccgaaga gcgtattatg aaattgccac
     9301 aagaggagcg ggcagcagct atcgctaatg tttacgcaca aaaacagcca atcgcaaatc
     9361 taacagaaca ctatgacttg attattaact tggtagatgt caatgctgga ggaacaacac
     9421 aacgtattat ttggccagca gctaaaggca cccctgatca gcctttttac gttcatgaaa
     9481 taccatctat tgtgatttca gtacaacatg catttgcttt agcagatatg ccacaggtag
     9541 ggacttatat taatgcttat gatgggcttc ctagcacgat aagtgcagta gttgcgaaat
     9601 tggctggaga atcagaattt acaggagttt caccagtaga tgcttactgt ggtttaattg
     9661 atacacattt atggcgccaa tgctaataga aacaagatga tgtactttta tgtaacataa
     9721 aaaaagagct taaagctctt tttttatcct tcaaaataga gtagttcctt gggtgttttg
     9781 gtaaatactt caaagccggt tttagtcaca taaccgcagt cttcaattcg cacaccaact
     9841 ttatctggta tataaatacc tggttcaaca gagaagcaca tgccttcttg aatctccata
     9901 tcgttaccag ccataataga tggaaattca tggacatcca tacctaatcc atgaccaagg
     9961 cggtggttaa agtattgtcc ataaccagcc ttttcaataa catttcttgc tgctgcgtca
    10021 acttcagaag caaggactcc tggcttaata aaatcaatag ccgcttgatg tgcttcaaga
    10081 cataaatggt aaatatcttt tttgaattga tcaggtttcc ccactgctac tgtacgggtc
    10141 atatctgacg tataacctag tgtttctaca cctaaatcaa ataataacaa agcattattt
    10201 tcgattttat tagttcctgg gataccatgt ggatttgctg cattattgcc agtcaaaacc
    10261 atagtgtcga aactcatttt attgatacct tgttttttca tttcaaattc gatttgagcg
    10321 ataatatcgg tttcagtgtt gtttagcgag atattgtcaa atccaacttg aacagcttta
    10381 tcagcaaatt ctccagcgac taacattttc tcaatttcat ctcgtgattt aattaggcgc
    10441 atgttttgaa tataaggtgt taggttttca aaatgaccat caaaaacggt ttggagacct
    10501 tggaatttag taacattaag attatcaaat tcggctagaa ctttagaaac agaaaaggat
    10561 ggtaagttgc tagctatttt ttgccaagga ttttctgaat ctatgtaacc aaagacggga
    10621 aatggtacac tttgttttgc acgcgatact tctagtgctg gtacaaataa aataggatcg
    10681 cgatcttcat agacaaataa aaacatttgt ctttcatggg gatcgcaaaa gaaacctgtt
    10741 aaatagttga ctgtcacagg atcagaaaag acagcaagtt cagcttggac tgaatgtaag
    10801 tgatggcgta tacgattaag ttttgacata ataacccttc ttcctaaaag gaacttagtc
    10861 tagttaggct agtttcttat gcctattatt tttccaaaaa aataggaaaa aatcaagttt
    10921 agttagtatt ttgaaagttt ttcataaaaa agctttataa aagcttgaaa gtgtttccaa
    10981 aaggtgataa aatatcagtg aaagcgtcat tttcaaatag aaaggaagtt aaatcaatat
    11041 gaatacagat gatacgatta cgatttatga tgttgcccgt gaagcaggtg tttcgatggc
    11101 aactgttagt cgtgtggtaa atggaaataa aaatgtcaag gaaaatacac gtaagaaagt
    11161 ccttgaagtt attgatcgtc ttgactatcg tccaaatgct gtggcgcgtg gtttagcaag
    11221 taaaaaaacg acaactgttg gtgttgtcat tcctaatatc gcaaatagct acttttcaat
    11281 tttagcgcgt ggtattgatg atattgcagc aatgtacaaa tataacattg tccttgcttc
    11341 aagtgatgaa gacgatgata aagaggttaa tgttgttaat actttgtttg ctaagcaagt
    11401 agacggtatt atttttatgg gacaccactt aactgaaaag attcgtgcag agttctctcg
    11461 ttcacgcaca ccgattgttt tagcaggtac tgtggacctt gagcatcaat taccaagtgt
    11521 taatatcgat tacaaagcag cagcagttga tgttattgat attttagctg gcaatcataa
    11581 agacattgct tttgtatcag gaccacttat tgacgatatt aatggcaagg ttcgtttggc
    11641 aggatacaaa gaaggtctca agaaaaatgg cttaaacttt aaagaggggt tggtttttga
    11701 agccaactac cgttatgctg aaggctttgc attagctcaa cgcgtgatta atgctggagc
    11761 aacagcagca tatgttgctg aggatgagtt agcggcaggt ttgttaaatg gtctttttga
    11821 ggcaggtaaa cgtgttcctg aagattttga aattatcaca agcaatgact cgccaatcgc
    11881 acaatataca cgtcctaact taacttctat cagtcaaccg gtctatgatt tgggtgctgt
    11941 tagcatgcgt atgctaacaa aaattatgca caaagaagag ttggaagaaa aagaaatagt
    12001 gcttaatcac gggattgtta aacgtggcac aacaaataat tagtgttaga gtatgttatt
    12061 tgaaaaatag tggtagaatg gtagtagctt agcgcttaac tttctgatac ttaacatgat
    12121 atattgagag gattagggta gcttagcttc ctaatctttt tttagaggtg taatatgaca
    12181 aatgaattga taatgcaagc ttttgagtgg tatttaccta gtgatgggaa tcactggaag
    12241 aaattagagg agtctatatc agaccttaaa aaacttggaa ttagtaaaat ctggttacca
    12301 ccagcattta agggaactag cagtgatgat gtaggatatg gtgtttatga tctctttgat
    12361 ttaggagaat ttgaccagaa tggaacaatt agaacaaaat atggtaggaa agaagagtat
    12421 ctaaagctta ttaagtcgtt aaaggcaaat ggcattaaac cgtttgcaga tatcgttctt
    12481 aaccataaag ccaatggtga tcataaagaa aaatttcaag tcatcaaagt caatcctgaa
    12541 aatcgtcaag aagcattaag tgaaccctat gagattgaag gatggacggg atttgatttc
    12601 ccaggtagac agggtgagta caatgatttt aaatggcact ggtaccactt tactggttta
    12661 gattacgatg ctaaaaataa tgaaacagac atctttatga ttgtaggcga taataaaggt
    12721 tgggcagacg atgatttgat tgatgatgaa aatggtaatt tcgattatct catgtacaat
    12781 gacattgatt ttaagcatcc tgaagttata aaaaacttac aagattgggc taagtggttt
    12841 attgaaacta caggcattga aggttttcgt ttggatgctg tgaaacatat cgattcttac
    12901 tttattcaaa ctttcattaa tgatattcgt acaaaaataa aacccgattt agaggtcttt
    12961 ggcgaatatt ggaaatctga ccaaacatca atgaaggact atttagaggc aacacaattt
    13021 caattttctc ttgttgatgt taccttacat atgaattttt ttgatgccag tcaccaaaac
    13081 cgtgattttg atatgcgaac aatttttgat gatagtcttg ttattgataa tcctgagtat
    13141 gctgttacat tcgtagaaaa tcatgatacg caaagtggac aagctctaga aagtagagtg
    13201 gaagattggt ttaaaccttt agcttatggc ttgatacttt tacgccaaca agggactcca
    13261 tgtctttttt atggtgatta ctatggcatt caaggagaat ttggtcaacc atcctttaaa
    13321 gaggttattg ataaaatggc tgagctacgc caaaactatg tatttggcaa acaagtcgat
    13381 tattttacgc attcaaattg tattggttgg acttgtcttg gggatgagga acataattct
    13441 tgtttagctg tggtcctaac aaatggtgat cagggatgga aacatatgga agtaggggaa
    13501 atttatgcag gcaaaacgtt tgttgattat ttaggtaatt gtgaacaaga ggttgttata
    13561 ggagatgatg gttggggtga ttttttagtt gaatctgcgt ctatctcggc ttgggttcct
    13621 aaaatagagg aagctaactg ataaatcagg tgaaaaaaaa ggtaaaatga gatataataa
    13681 gacctatgaa agttttactg tatttagagg ctgaagaata cttgaaaaag tcaggtatcg
    13741 gtcgtgctat caaacatcaa gagaaagctc ttcaaattgc tgggatagat tataccacta
    13801 atcctacaga tgactttgac cttgttcata tgaataccta tggtattaga agttggcttt
    13861 tgatgagtaa agctaaaaaa actgggaaaa aagtaattat gcatggccat tccactgaag
    13921 aggactttag aaattctttt attggctcaa atttagtatc cccgcttttt aaatggtacc
    13981 tttgccgttt ttatcaaaaa gcagatgcca ttataacccc aactgattat tctaaacaac
    14041 taataaaagc atatggcatc aagaaaccaa tttttgtttt atctaacggg attgatctaa
    14101 gtcgctatca gaggtcagaa aagaaagaat ctgcatttcg tcactatttt cacttaagta
    14161 aggatgataa agttgttatg ggggccggtc tttatttcat gcgtaaggga attgaccaat
    14221 ttgttgaagt agctgcaaaa atgcctgata ttcgttttat ttggtttgga gaaacaaata
    14281 aatgggttat tcctcgaaag gttaggcaaa tcgttaccaa gcaacatccc tcaaatgtta
    14341 cttttgctgg ttacataaaa ggtgatgttt atgaaggtgc catgagtgct agcgatgctt
    14401 tcttctttcc gagtcgtgaa gagacagaag ggatagtagt tttagaggct ttagctagtc
    14461 atcagcatgt ggttttaaga gatatccctg tgtatcacgg ttgggtgacc gaagacagtg
    14521 ttgaattagc gactgatgtt gatggttttg ttgaaaagtt ggataaagtc ttatctggta
    14581 agagtgataa gattaaagaa ggttatcatg tggctgagag tcgaagtatc gaacgaattg
    14641 cccatgaatt ggcaagtgtt tatcaaaaag taatggagtt gtaagatgcg tataggtcta
    14701 tttacagata catacttccc ccaagtttcg ggagtttcaa caagtatccg cactttaaag
    14761 gaagggttgg agaaggaagg acacgaagtt tatattttca caactaccga ccgtaatgtc
    14821 aaacgttttg aagaccctac aattattcgt ttaccaagtg tcccttttat ttcttttacg
    14881 gatcgtcgag tagtttatag aggtttaatt tctgcttata ggatagctaa agactacgaa
    14941 cttgatatta ttcatactca aacagaattc agcttagggc ttttaggaaa attagttgca
    15001 aaagctttaa gaatacctgt tgtgcatact tatcatactc aatatgagga ttacgttggt
    15061 tatatcgcaa aaggcaaatt gattaagcca agtatggtca aatatatcat gcgtacctac
    15121 ctaagtgatt tggatggtgt catatgtcct tctcgaattg ttctcaatct tctagatggt
    15181 tacggtgtca aaattccaaa acaagttatt ccgacaggta ttcctgttga gaactatcgt
    15241 cgagaggata tctctgaaga aacaatcaag aatctacgaa cggagcttgg acttgcggat
    15301 aatgatacaa tgctattgag tttatcacgg gtatcttttg aaaaaaatat tcaagcaatc
    15361 ttgatgcatt tatcagctgt cgttgatgaa aatcctcacg taaaattagt gatagttgga
    15421 gatggtcctt atctttcaga tctaaaagaa cttgttcact ctttagaact tgaaaattca
    15481 gttattttta caggtatggt tgagcatagc caagtggcta tctactataa agcatgtgat
    15541 ttctttatta gtgcttcaac aagtgaaacg caaggattaa cttacattga gagtctggcg
    15601 agtggacgtc caatcattgc gcaaagtaat ccttatctag atgatgttat ttcagataaa
    15661 atgtttggaa ctctgtacaa gaaggaaagt gatttggcag atgctatcct agatgcaata
    15721 gcagagacac ccaaaatgac tcaagaagct tatgaacaga aattatatga gatttctgct
    15781 gagaatttca gcaagtctgt atatgctttt tacctagact ttctgatttc acaaaaagct
    15841 agtgtgaaag aaaaagtctc tctgacaata ggaaataagg atagccattc tactcttcgt
    15901 tttgtcagaa aagcggtata tttacctaag aaagttttta cctttacagg aagagcctct
    15961 aaaaaagtgg taaaagcacc taaacgtcgt atttcatcta ttcgagattt tttagattga
    16021 gacgaataaa attagtaaat gtcttgaaat tataaaaaat attgttataa taaaaaatga
    16081 agacatgtta ggttctaata gattttaaga gcgagcactg gttggtggaa agagtgcaat
    16141 tagagaactt agtactactt ccttgatttt atgcaaacat aaaagggtgg ttcccttatc
    16201 gccactagag gtctgacaca agtatactcg agtttttgtg ttggataaat tgaggtggaa
    16261 ccacgtagtc tttacgtcct tttggatgta aaggctcttt ttttataaaa gaagtggttg
    16321 cataaaagag gcagattgct ttgctaatgt tatacttatt atccctagct aggataatgt
    16381 ggaattcaat caaaggtcaa tccctttgtt tattagtgaa ttaatgtgtt taggaattgg
    16441 tatcatccta acagttatta agaaaaggag aacaagatga ttaaaatcac tttcccagat
    16501 ggtgcaattc gtgaatttga atctggcatc acaacatttg aaattgcaca atcaatttct
    16561 aactctttag ctaaaaaagc tttagctggt aaatttaatg gtcaacttat tgatacaaca
    16621 cgtgctatcg aagaagatgg tagtattgag attgtaacac ctgaccacga agatgcttta
    16681 ggagttcttc gtcactcagc agcgcattta tttgctcaag ctgcaaaacg cctttttcca
    16741 gacttatgtt taggtgtagg tccagctatt caagatggtt tttactacga tactgataat
    16801 aaatcaggtc aaatttcaaa tgatgatctt cctcgtattg aagaagaaat gaaaaaaatt
    16861 gttaaggaaa atcacccttg tattcgtgaa gaaatcagta aagaagaagc gttagaactt
    16921 tttaaagatg acccttataa agttgaatta attagtgaac atgctgaaga tggtttaact
    16981 gtttatcgtc aaggtgaatt tgtagacctt tgtcgtggac ctcatgtccc atcaacagga
    17041 cgtattcaag tctttcatct tctaaatgtc gctggtgctt actggcgtgg taacagtgat
    17101 aatgctatga tgcagcgtgt ctatggtact gcttggtttg ataaaaaaga tttgaaagca
    17161 tatcttaaac gccgtgaaga agctaaggaa cgtgaccatc gcaaattagg taaagaactt
    17221 gatttattta tggttaatcc tgaagtaggt caaggattgc cattctggtt gccaaatgga
    17281 gcaactattc gccgcgaatt ggaacgttac attgttgata aggagattgc ttcaggttat
    17341 caacacgttt acacacctcc gatggcatct gttgaatttt acaagacttc tgggcactgg
    17401 gaccactatc gtgaagatat gttcccaaca atggatatgg gagatggaga ggaattcgtt
    17461 cttcgtccaa tgaactgccc acaccatatt gaagtttata aacatcatgt tcattcatat
    17521 cgtgaattgc caattcgtat cgctgaactt ggtatgatgc accgttatga aaaatcaggg
    17581 gccctcacag gattgcaacg tgtacgcgaa atgacactca acgatgccca tattttcgtt
    17641 acacctgagc aaattaaaga tgaatttctt aaagctctta atcttatagc agaaatttat
    17701 gaagatttta acttaacaga ttaccgtttc cgcttatctt atcgtgaccc agaagataaa
    17761 cataagtatt atgataacga tgagatgtgg gaaaatgcac aagctatgct aaaagaggct
    17821 atggatgatt ttggattaga ttattttgaa gcagaaggcg aagcagcctt ttacggtcct
    17881 aaattggata tccaggtgaa aactgctctt ggtaatgaag aaacactatc tactatccaa
    17941 ctagatttcc ttcttccaga acgctttgat ctcaaataca tcggtgcaga tggtgaagaa
    18001 catcgtccaa ttatgattca ccgcggtggt atttcaacaa tggagcgctt tacagctatt
    18061 ttaattgaaa cttacaaagg tgcctttcca acttggcttg ctccacaaca agtttctgtt
    18121 attccaattt caaatgaagc ccacattgat tatgcttggg aagtggctcg tgtccttaaa
    18181 gatcgtggta ttcgtgctga agtagatgac cgtaacgaaa agatgcaata taagattcgt
    18241 gcggctcaaa cccaaaaaat tccatatcaa ttgattgttg gtgataagga aatggaagaa
    18301 aaagccgtaa atgtgcgccg ctatggaagc aaagcgactg aaacaaaatc tatagaggaa
    18361 tttgtagaaa gtattttagc tgatattgca agaaaatcta gacctgacga agttaaataa
    18421 attttattag tcattataaa aatcattaat aaatgatgtg aactgattac tgtatgatgc
    18481 agaatcagtt ttttagatgc taattttttc tttatgttta attataagct aattatagat
    18541 aaataatctc atataacatt cacaaaatta gatttttttg gtataattaa tatgattgac
    18601 acatgaagga aaatagtatt aataaaaagg ggtatggagc attgcgaaca gtattagtag
    18661 tgcaaggtga tgacgaaaca atagagttat taaggtcgta tttagaggga gctctttata
    18721 aggttgtgat ggctagtgat ggtgaggagg ctttttcttt atttcagcaa catcagattg
    18781 atttagcaat catagatatt acgctaccta aaatagatgg atatgagttg acgcgactca
    18841 ttcgtcaaga tagtcagatt cctattatta tgctagcagc taagacaaca gatatggaca
    18901 gaatattagg attgaatatt ggagcagatg attttatcac gaaaccgttt aattctttgg
    18961 aggttttagc tcgaattaat tcgcaactgc gtcgttacta tgaattcaat agtcttgcca
    19021 aacctaaaaa tcaatttata aagatagggg aattggaatt agatgaggaa catgttgaac
    19081 taactaaaaa tggtaaacat atcaagttga ctgctacaga atttaagatt cttcatatct
    19141 taatgtcatc accaggaagg atttatacta aaacacaact ctatgagaag attaatggta
    19201 ggtatttaga aggtgatgag acaacgatta tggtgcatat ttctaatatc cgtgacaaaa
    19261 tcgaagatga tagtaaatat cctaaatata taaaaacact tagaggagta ggatataaaa
    19321 tcgaaaaacc gcagtatctt gtttaaaaat ataatggcta gtaagagcca ttttttagtg
    19381 aagatatttc ttggttttaa gctttccttt ttgctataat aagtaaaatt ggaggggaga
    19441 gatatggcaa agaccgctct taatattcct tttacagttg aagggataga aaattattat
    19501 caaaatataa aaatgatact gtctttagca gataaatgga gcatacctaa agatgatcta
    19561 ttactttttt taaagggcct caaggctatt gatgatataa aagttggctc tcctattttt
    19621 aagtattttt ggacttcttt aagtttacaa gcacccctca aagctttaga attcgtgtta
    19681 gagcaggcta aaatgccaac tgaattgtca ggagaattat cagaaacaca atacttggtc
    19741 gcacagtttt cagatgagtt agctccacat gatgatttct ggatagcctt atcacaagtt
    19801 atttatgatt cttttcctgg gaatagtcta gccgaagata ctgtattaaa taggaaactc
    19861 catcaattcc gctatttgat ttctagtcag caagcacagt atgttcgacg atactttaaa
    19921 gatgttggta tgactgatag agatgcttta gtaaactacc tatcatgttt aagagaacca
    19981 gatagtatcg cctactatga atctgctcgc ttgcataata aacgtcggag aaatggtgaa
    20041 atctttggtt tcccagatga tgagccggtt attaatagta agcttttaat ttcattccat
    20101 acggaattta ttattgatga caaaggaaac tttttaaacg agattgacgc agaagtaata
    20161 acacgtaatg gtattatcaa tggagctagc tttaattatg cttttaaaaa taatacacgt
    20221 cataaagaat tagatgttga tcctgtaaag ttagacccaa aatttcgaaa cgatatgact
    20281 agaggttata gatcgccaaa tctatctaga aggaaatggt ttttctttaa agaagaggat
    20341 tacgattgta gttatttcaa taaaaaagga tattacgcat ttggaaggcg tagtgctaag
    20401 caatcggttg ataaacaagt caaatacttg aagaaggctg tgcagaaaat gcgcatcaac
    20461 tgatacatat actattaaaa gataccatag tactatggta tcttttaatt tgaggtatta
    20521 atgtcaaatg gttttttatt gaccacatgt ttttataaac gagtggtcaa aattgaagaa
    20581 gtatctagct tatgttaaaa tataattgaa taatagaaag aaggtgctgt atgattaaga
    20641 aaaataaagt ttttttagga gttcttttag tccttgttgt tatccttgga ggtggtgtat
    20701 tattttacca atcacaattc caaaagacaa ctaatcaagc gttagctatt gcttataaag
    20761 atgctaaagt ggctaagaag gatgtcatac atcaaaaaat tgacaaagaa tttgaaaact
    20821 ttagagggag ctacgaaatt gaattcaata ccaaaagcgc agaatacagc tatcatgtag
    20881 atgttaagac tggacaaatc cttgagcggg acatggataa taatggcttt tcaaaatcga
    20941 cttctcagtc aagttcatcg tcaagtcaaa aatctcataa aataagtcaa gaagaagcca
    21001 aaaagattgc atttaaagat gctaatatag aggaatcaga agtcagtaat cttaagatca
    21061 aagaagaaat tgaaaatgga aaatcagttt atgatattga ctttgtggac ctgaaaaata
    21121 aaaatgaagt tgattatcaa attgatgcag aaactggaaa aatcattgaa cgttctagag
    21181 atcatatgaa tgattaattt aaataagata taaaaaaacg aaggtctaaa aggccttcgt
    21241 tttaattact cagtagcttg ctcccaacgt tttgaaagtt tacgtgaaaa acttgagatg
    21301 ataaagtttg taatgaaata gacagcagtt ataatagcgt agagtgtaaa aacttgtcca
    21361 gcttcgaaat atcttcccat tagaatttga ctttttccga agatttcttg aatagctata
    21421 acagaataaa gtagtgatgt atcttttata accgttacaa actgagaaat aatggctggc
    21481 aacattttgc gaaaagcttg tgggaaaata ataataatga aaacttgcaa ataagtgaat
    21541 ccttgagaaa gtccagcttc tgtttgtcct ttatcgactc catttaaacc tccccgaata
    21601 atttctgcta aagcagcgga ggtaaaaatt gtaaacgaag taataccagc gctgactgat
    21661 ttcatctgga atactaagaa aatgataaaa atccaaagta gatttggaac atttctgaca
    21721 aattcgatat agatactagc tattaatttc cagataggat ttttgctatt tctcataatt
    21781 gctaatagag ttccaaaaaa cattgatagt aaaatggaaa taaaagatat atataaggtt
    21841 aaaccgaacc cttgaaggag aaatagtatc tttgttagtg ttaagactga tgacatatca
    21901 aacctcctta agaataagtt ttcttattta attcttcttg ttttcttgcc cagcttgcta
    21961 gtgggaaaca aataacaaaa tagaagatag cagctcctgc aaaagctggg atataattag
    22021 tcgtatcata agcccaggcc ttagctacaa acatgatatc ggctccagaa atgattgcta
    22081 cggtagaggt attcttgatt agattaacaa cttggttggt cattggtggt aaaattgttc
    22141 tgacagcttg aggcaaaatg ataagttgca tggtttgatt agctgtgaat ccttgagcta
    22201 aagcagcctc tgtttgtcct ttagggacag cttcaattcc tgagcgaata acttcagaaa
    22261 tataggcacc atggtatagt ccaacgcaca aaacggcggt gaaaaaagca ctaatcataa
    22321 catgtccatt actaatgatt gctagtccat agtagacaaa aaccatttga actaaaagag
    22381 gtgtgttttg aaaaacttca acatagatac gtgatatcaa cttaccaact ttacttttag
    22441 aagttgataa accaccagag agaattccta atatcagagc aagtaggagt gcaccaaacg
    22501 aaatacctag agtatataag aagcctttaa agaataggtc gaaatggtta aagaaagcgc
    22561 cccaccttga aatggcaaaa ggactgatgt ttaataaagt cataaaaaat cctccctatt
    22621 ctttttttac tttagcgggt ttaagcttaa atttttgata aatgtgtttt aaactaccat
    22681 ctttttccca tgataccagt aacttattaa cgtagggaat caagactttg tcctgtttac
    22741 tggttgcaat accgtattct tgcgtattga acccttcttt taatatggtt gtatgagggc
    22801 taaggtagcc gctaaggata gatttatcaa ctgaaaaagc atcaatacga ttagcgtaca
    22861 gcgatatcgc taattctggg aaactcccta attgagcata gctaaatttt agcttgtgtt
    22921 ctttagcata tttttctaaa ttgacttttg tagtggcacc ttgagccaca ccaatgtgtt
    22981 taccatccaa ttcttttata gttttgatat gactattttt acgcaccaaa aatccaattt
    23041 catcgtgata ataagcttta gaaatattat agttagcttt tctttcgggc gtgatagtgt
    23101 atgttgcgat tagaatatca atttgcccat tgtccatcag aggctctctg gtttgtgcgg
    23161 ttgtcggtac aaaaactggt ttgacaccta aggatttggc aatttttttg gcaatatcaa
    23221 tttccattcc ttcgtattga ttcgtctcag cattataata accaaagtta ggcacatctt
    23281 gtttgactcc aactttaagg acgcccgatt ttttgatttc tgataattga ttagctgacg
    23341 ttgagttagg ataaaataac aggaaaataa aaatacagga taatagatag aacgttaatc
    23401 tttttcgtct cagcatattt cgtcctccta aaggtcagta ctggtatggt tgataatgtt
    23461 gcttaaaaat tgtttagcac gtggttctct gggattatca aaaaagtctt gtacatctgt
    23521 agtatccact aaaatttccc catcagccat aaaaataata cgatcagcaa cttctcgtgc
    23581 aaatcccatt tcatgagtta caacgaccat attcattccg tcatttgcca atttttgcat
    23641 cactgaaaga acgtctccga tggtttcggg atcaagggca gaggtaggct catcaaagag
    23701 gaggagtttt ggatgcatgg caagccctct agcaatagca attctttgtt tttgaccacc
    23761 tgatagcata gatgggtagg aatcttttct ttcccacata ttgacaaatt ttaaatattt
    23821 ttcagcaatt tccatagctt cttttttaga ttgttttaat actttaatag gagctaatgt
    23881 aatattttct aacacggttt tgtgaggata aaggttaaaa tgttgaaaaa ccataccaac
    23941 ttctttacgg aggttgacca attctttaga tgaaatattt gctaattcat gtccattcac
    24001 gaccaaacta ccatcgtcaa tagattcgag agcgttcatt gttctaataa gtgttgattt
    24061 acctgatcca gaaggtccta atagaacaac cacttgccct ggctctattt ccaaattaat
    24121 ctgacgaagg gcgtgataat ctccataata cttgttaaca ttcttataag atatgagtga
    24181 cataagaacc tcctaaaata acagtttatt gttaggttac ctaacattat agataagtgt
    24241 atcacctgtt tgtgagcttg tcaataaatt cagaatatta aagagtagga ttgatttaaa
    24301 tattgctatt tttagagcgg tatttttagt aaaaatatta cgttctcgta tcatttattt
    24361 ttcagtttaa taggatgtat tgaaagtttc caattaacta ggaaatctgt tataattagt
    24421 gataatagtg gtgctttagg cccatattat catattttcg gtcactttta tttttcagtt
    24481 tatgaatgga agtgggagac cagaaagtaa tagaggtaaa atatgaaaaa aatacttatc
    24541 gttgatgatg aaaaaccaat ttcagatatt atcaaattta atttaaccaa agaaggatat
    24601 gagacggcga cagctttcga tggccgtgaa gctttagtac aatatgcaga atttcaacca
    24661 gatttaatca ttttggattt aatgcttcct gaactagatg gtttagaagt ggctaaggaa
    24721 gttcgtaaaa ccagccatat tcctattatt atgctttctg caaaagacag tgagtttgac
    24781 aaagtcattg gtttagaaat cggtgcagat gattatgtta ctaaaccttt ttcaaatcgt
    24841 gaacttttgg cacgggtcaa agcacattta cgacgtactg aaaatattga aacagctgta
    24901 gcagaagaaa gtgctcaaaa tgcttcttca gatattacta ttggtgagtt gcaaatttta
    24961 ccagatgcct ttattgctaa gaaacgtggc gaagaaattg aattaacgca ccgagaattt
    25021 gaattacttc accatttagc gacacatatc ggacaagtca tgacacgcga acatttattg
    25081 gaaacagttt ggggatatga ttattttgga gatgtccgta cagttgatgt gacggtgaga
    25141 cgtttacgtg aaaagattga agatactcct ggtcgtccag aatatatttt gactcgacgt
    25201 ggtgtaggtt attacatgaa gtcttatgaa taatagtgct gcaaatatta ggtcgtttga
    25261 gctagcctta ctttttttac tagtttttgt tgctgtttat tttgtttatc tagcggttag
    25321 agattttaaa atgtctaaaa atatccggct tttgaattgg aaagtcagag atttgatagc
    25381 aggaaattat tcagatagta ttctaattca aggtgatgca gatttggtag aattaggtga
    25441 aagtctaaat gatttatctg atgtcttccg aatggcacat gataacttag aacaagaaaa
    25501 gaatcgctta gcaagtatct taacttatat gactgacggt gtgctggcta cagatcgttc
    25561 aggaaaaatc gttatgatta atgagacagc ccaacagcag ttcaatctag catatgatga
    25621 agctttatca atgaatattg ttgatatgtt agggtcggga agtccatatt cgttccaaga
    25681 tttagtctct aaaacgccag aggtagttct taatagacgt gatgaaaacg gtgaatttgt
    25741 taccttacgg attcgatttg ctttgaaccg tcgtgaaagt ggttttattt ccggtttagt
    25801 tgcagtgtca cacgatgcta ctgagcaaga gaaagaagag cgtgaaagaa gactttttgt
    25861 atctaatgtt tctcatgaac ttcgtacacc tttgacatct gtaaaatctt atttagaagc
    25921 tttagatgag ggagcgctta atgaagaagt tgctccaagc ttcattaagg tatctctaga
    25981 tgaaacaaat cgtatgatgc gtatgatatc agatttatta agtttatcgc gcattgataa
    26041 tgaagtaacg catttagatg ttgaaatgac gaattttaca gctttcatga cctcaatttt
    26101 gaatcgattt gatcagatta gaaatcaaaa aacagtcaca ggaaaagttt atgaaattgt
    26161 cagagattat cctcttaagt caatttgggt ggaaattgat acagataaga tgactcaagt
    26221 gattgataac attttaaata atgcagtcaa gtattcacca gatggtggta agatcacagt
    26281 taatctacgc acaactaaaa cgcagatgat tttatcaata tcagaccaag gcttaggtat
    26341 tcccaaaaaa gatttacctc tcatctttga tcgtttttat cgtgttgata aggcgagaag
    26401 tcgtcaacag ggtgggactg gacttggttt gtcaattgca aaagaaattg ttaagcagca
    26461 taagggattt atttgggcta agagtgagta tggtaaaggg tctactttta caatagtctt
    26521 gccttatgat aaagatgctg taacttatga agaatgggag gacgttgaag attaaaacat
    26581 gtcagaaata ggatttaaat atagtatctt agcttcaggt tcaactggaa attgttttta
    26641 tattgagaca ccccaaaaac gtcttttaat tgacgctggt ttaacaggca agaaagtgac
    26701 tagccttctt gcagaaatta atcgtaaacc agaagattta gatgctatac tagttaccca
    26761 tgaacattct gaccacatta aaggtgttgg cgtattggct aggaaatatc accttgatat
    26821 ttatgccaat gagcagacgt ggaaagttat ggatgagcgt aacatgttag gcaaagttga
    26881 tgttagtcaa aagcatgttt ttggacgcgg taaaacctta actttcgggg atttagatat
    26941 tgagagcttt ggtgtaagtc atgatgctgt ggatccccaa ttttaccgta tgatgaaaga
    27001 tgataagagc tttgtgatgt tgacagatac aggatatgtt agcgaccgaa tggcaggctt
    27061 gattgagaat gcagatggtt atttgatcga gtcaaatcac gacattgaga ttctacgttc
    27121 gggttcttat ccttggacac ttaagcaacg tattttatca gataaaggac atttatctaa
    27181 tgaagatggt tcagaaacca tgattcgcac tataggtaac cgtactaaac acatctattt
    27241 aggacacttg agtaaggaaa ataatattaa ggaacttgct tacatgacta tggaaaacaa
    27301 tttaatgaga gctgactttg gtgtgggcac agatttttct gttcacgata cttcacctga
    27361 ttcagctacc ccactgacga ggatttaaga ttgaaaaaga ttttttacat tagtttagga
    27421 tttattagtt tagggatagg cattgcaggt attgtcttgc cggtagttcc aacgactcca
    27481 ttagtattgc tttcagcttt ttgcttttcg agaagctctg aaaaatttga tatttggttg
    27541 cgtcaaacta aagtttataa atactacgct gcagattttg ttgaaagcag aagtattgcc
    27601 ccagcgcgta aaaagtcaat gatttggcaa atttatatct tgatgggaat ttcaatctat
    27661 tttgctcctc taatgtggct caaactggga ttattgattg gaactattgt aggaacttat
    27721 gtgctttttt atgtagtacc tgataaaatt gatgaatagt taacaagaga ctaggtgtgt
    27781 atcctagtct tttgttgaat attcaaatat gtaattaagg aaacgttttc tttggaagat
    27841 aggtttaact tccaaaatgt ttgattattt gtcaactgtg gtataatggt atttgtttaa
    27901 tattttatct atgattgaaa tcaaggagaa aaaaatgaaa gaattaagat caaagctgga
    27961 aaaagactat ggtattgttt ttgctaatca agaattatta gatacagctt ttacccatac
    28021 atcatacgca aatgagcatc gcctcctaaa catttcacat aatgaacgtt tggaattttt
    28081 aggagacgct gttctacaat tacttatttc acagtatctt tttacaaagt accctcaaaa
    28141 ggcagaaggt gatttatcaa aattacgttc aatgattgtt cgtgaggaaa gtcttgcagg
    28201 tttttcacgt ctatgtggct ttgatcacta tattaaactt ggaaaaggag aagaaaaatc
    28261 tggtggacgt aatcgtgaca ctattttagg agatttattt gaggcttttt taggagcttt
    28321 attattagat aaaggtgttg aagtggttca tgcctttgtt aacaaggtaa tgatccctca
    28381 cgttgaaaaa gggacctatg agcgtgttaa agattacaag acaagcttgc aagaattact
    28441 tcaatctcat ggggatgtaa aaatagatta tcaagttact aatgaaagtg gtccagcaca
    28501 tgcaaaggaa tttgaggtga ctgtatctgt caaccaagaa aatttgagcc aaggaattgg
    28561 acgatcaaag aaagctgctg aacaagacgc tgccaaaaat gctttagcaa cgttacaatg
    28621 aggaaatcat gtttttaaaa gaaattgaaa tgcaaggctt taagtccttt gcggataaaa
    28681 ctaaggtaga atttgatcaa ggagttactg ctgttgtagg tccaaatgga tcaggtaaaa
    28741 gtaatatcac agagagttta cgttgggcat tgggagaatc aagcgcaaaa agtttacgag
    28801 gaggtaagat gccagacgtt atatttgctg gtacagaaaa tcgtaagccc cttaattatg
    28861 cgcaagtgag tgttacttta gataatagcg accattttat cgaaaatata gctgacgaag
    28921 ttcgagttga acgtcgtatt tttcgaaacg gagatagtga atatttaata gatggacgca
    28981 aagttcgctt acgtgatatt catgatttat ttatggatac tggattagga agagattctt
    29041 tttctattat ttcccaagga cgcgttgaag ctatttttaa tagtaaacca gaagagcgcc
    29101 gtgccatatt tgaagaagcc gctggtgttc tgaaatacaa aacacgtaag aaagaaacac
    29161 aatccaaatt agaacaaact caagggaact tagatcgctt agaggatatt atttatgaat
    29221 tggacatgca agtccaacca ttagaaaaac aagcaagtat tgccaagcgt tttttagtat
    29281 tagatgagga acgacaagga ctccacttat ctattttgat tgaagatatt ttacaacatc
    29341 aatcagattt aactactgtt gaggaaaagc tattgacagt aagaaaagag ttagcaactt
    29401 attaccaaca acgtcagtcc cttgaagatg aaaatcaatc gttaaaacag aaacgtcacc
    29461 atttatctga agaaattgag gctaaacagt tagctctttt agacgttaca aagttaaagt
    29521 cagatttgga acgccaaatt gatctgattc gtttagagtc aaaccaaaag gctgaaaaaa
    29581 aagaagaggc aggtcaacgc ttagcagagt tagaaattaa agctaaagat tgtagtgacc
    29641 agattaccca aaaaaatata gagttaacta ctctgtcgga aaaaatagcc caaataagat
    29701 cagaaattgt tagtacagaa agttccttgg agcgtttttc aactaatccg gatcagatta
    29761 ttgaaaaact tcgagaagac tttgttacct tgatgcaaga agaagccgac acttcaaacg
    29821 ctttaacagc tttactagct gatatagaga atcaaaaaca agcttctcaa gctaaatctc
    29881 aagaaataca agaagttagc aaaaatttag aagtattaaa aagtaacgca aaggtagcat
    29941 tggaacgatt tgaggctgca aagaagaatg ttcgtcagtt attatctcac taccaagatt
    30001 taggtcaaac cttgcaaaat ctagaaggtg agtataagaa tcagcagtca attttatttg
    30061 atcatttaga tgaaatcaaa tcaaagcaag cacgtatttc gagtttagaa tctattttga
    30121 aaaatcatag taatttctat gctggagtga agtcagttct acaggctaag gaccagcttg
    30181 gaggtatcat aggtgctgtt agtgagcatc ttagctttga caaacattac caaacagcat
    30241 tggagatagc tttgggaggt agcagccaac acatcattgt tgaagacgag agtgctgcca
    30301 aacgttcaat tgctttttta aagaaaaacc gccaaggtag agcgacattt ctgcctttaa
    30361 ctacaataaa accaagagaa ttagcacaac attatctctc aaaattacaa agttctcaag
    30421 ggtttttagg aatagctagt gaattggtaa cctatgatca acgcttgtca aacattttta
    30481 aaaataactt agggttaact gctatttttg atacggttga taatgctaat gtagctgcga
    30541 gacaattgaa ttatcaagta cgtttagtaa ccttagatgg aacagagtta cgccctggag
    30601 ggtcgtactc agggggagca aatcgtcaaa ataatactgt ttttatcaag cctgaattgg
    30661 ataatctaaa aaaagaattg aaacaggcac aaagtaaaca attaattcaa gaaaaagaag
    30721 ttgccacatt gttagagcaa ctcaaagaga aacaagaaac cttagcacaa cttaagaatg
    30781 atggagaaca agctcgtctt gaggaacaaa gagctgatat agagtatcaa caactatcag
    30841 aaaagcttgc tgatttaaat aaactatata atggcttgca attaagcagc ggggcgttgg
    30901 aacaaactac ttctgaaaat gaaaaaaatc gtttagaaaa agaacttgaa caatttgcta
    30961 ttaaaaaaga agagttgaca acttcaattg ctcaaattaa ggaagataaa gattcaattc
    31021 aagaaaaagt caataatcta acaacattat tgtcagaggc acagctagaa gaacgtgatt
    31081 tgctaaacga gcagaaattt gaacgtgcca actgtacacg tctagagatt accttgtctg
    31141 aaataaaacg tgacattagt aatttacaaa cattactaag ccatcaggac agtcaattag
    31201 acaaagaaga gcttccacgt atagaaaaac agttgctaca agtaaataat agaagagaaa
    31261 atgacgaaga aaagcttgtg agtctccgtt ttgaattgga ggattgtgaa gctgctttag
    31321 atgatttagc tgcttctctg gctaaagagg gacaaaaaaa tgaaagtctt attcgtcagc
    31381 aagctcaatt agagtcacaa tgtgaacaat tatctcaaca attgatgata ttctcacgtc
    31441 aattatcaga agactatcag atgacattgg atgaagcgaa ggtaaaagca aatgtcttgg
    31501 aagatatttt gatggcgcgt gaacaattga aatctctaca agctaaaatt aaagctttag
    31561 gacctgttaa tattgatgca attgctcaat ttgaagaagt tcatgaacga ttgaccttcc
    31621 ttaacacaca gcgagacgac ttagttcatg ccaaaaattt attactagag acgattacag
    31681 atatggatga tgaagttaag actcgtttta aatcgacatt tgaagccatt cgtcatagtt
    31741 tcaaagaaac ctttgtacag atgtttggtg gaggttctgc ggaccttatt ctgacagaag
    31801 gtgatttatt atcagctggg gttgatattt cggttcaacc tcctggtaaa aaaatccagt
    31861 ctttaaactt gatgtcaggt ggtgaaaaag cattatcagc gttagcatta ttatttgcca
    31921 ttatccgtgt taagacaatt ccatttgtca tcttggatga agttgaggcc gctttagatg
    31981 aagcaaatgt taagcgattt ggcgattatc ttaatcgatt tgataaatca agtcaattta
    32041 tcgttgttac tcaccgaaaa gggactatgt ctgctgctga tagtatctat ggggtaacga
    32101 tgcaagaatc aggcgtttcc aaaattgttt ctgtaaaatt aaaagaagct caagaaatga
    32161 ccaattagaa aacagaaggg gatcccttct gttttttgga ttttcagcct tattttatgt
    32221 tacaatagta aatgtaaacc ctttagttag gagattccat gataaaatta gtagcaaccg
    32281 atatggacgg tacattctta gatgaaaacg gtacctatga taaaaaacgt ttagcaaatg
    32341 tgctaaaaaa atttaaagaa caaggaattg tctttacggc agcaagtggg cgttccttat
    32401 tatcacttga acaattattt gctgactttc gtgatcagat ggcttttata gcagaaaacg
    32461 gaagtgcagc agtcttattt aatcgcttgg catatgaaca gcacttgagt cgagaacaat
    32521 acttagatat tattgaccat ttgagtaaga gcccttatat ggagaataat gaatatgtct
    32581 tatctggcaa ggatggtgca tatattctaa gtgatgccaa tccagattat attgaattca
    32641 tcactcatta ttatgataat ttgcaaaaag tttctcactt tgaagatgtt gatgatatta
    32701 ttttcaaagt aacggctaac tttactgaag agactgttag acaagctgag gagtgggtaa
    32761 atcaagctat accgtatgcc acagcagtga caacaggttt taaatccatt gatattattc
    32821 taagctcagt taataaacga aatggtttag agcacctctg tgaacaatac ggtattagag
    32881 cagaagaagt cttatctttt ggtgacaata ttaatgattt agaaatgcta gagtggtcag
    32941 gaaaagctat cgcaacagaa aatgctagac cagaagttaa agagatagca gattgtatta
    33001 taggtcatca caataatcaa gctgtaatgg cttatttaga aagtatggtg gattaatgtc
    33061 ggatattaaa atacttgcct tagatttaga tggcacttta tttacaacag ataagaaagt
    33121 ttcagaagaa aataaagtgg ccttaaaagc agctagagaa aaaggtataa aagttgttat
    33181 tacaacaggg cgtcctttaa aagcaattgg taatttgtta gaggacctag agttagtatc
    33241 tgatgaagat tacagcatta cttttaacgg tggtttagtt caacaaaata cagggaaaat
    33301 cttagctaaa actgcaatga cacgacaaga agtggaagat atccatgagg agttgtatca
    33361 agtgggtttg ccaacagata tcttaagtga gggaacggta tatagtatcg ctaacaaggg
    33421 acaccattct caatatcatt tagccaaccc gctcttagaa tttatagaag ttgatgattt
    33481 agagcaagtg ccaaaggatg tagtttataa taaaattgtt agtgttattg atgctacata
    33541 tcttgatcaa caaattgcta aacttcctga tagacttaaa gttgactatg aaatgtttaa
    33601 atcacgtgat atcattttag aattgatgcc taaaggtgtt cataaagcag tcggcttaga
    33661 actactgaca aaacaccttg ggttagatag tagtcaagtt atggcaatgg gagatgaagc
    33721 aaatgattta tcaatgctag agtgggcagg cttaggagtt gcaatggcta atggtattcc
    33781 agaagctaag gcaatagcaa aagcgacaac tatttgtaat aacgatgagt ctggagtcgc
    33841 cgaagcaatt gggaaatata ttttaagtga ggaaaattaa tgggattatt tgaccgccta
    33901 tttggacata agaaaaaaga taaagaacca gaaattgaag catcagaatc tgttgtttta
    33961 gaagatgaag attcagttat tgataaagag gagggcagca acttttcgaa ggagtcaact
    34021 ttgaatcgaa cctctgaagt ccctgttgca gaagatgatt ctttcttaga attagaaaga
    34081 gatacagcat tgtcagaatc acatcaacca gttacctctg aaattcatcc tttagagtca
    34141 gaagataccg atgaaattcc tgtaaaagaa gacgatagtt ttttagaact agaagatagg
    34201 gctaagacaa aggtagcaga tacttctgag gtagaaaatg tagttcctga ttccacgact
    34261 ctttctgaca atgtttctgc taaatctgag gcatctttta gtgataagga gcaattaagt
    34321 gatagtcagg cttcagatca gttttcagaa actccccttc aagaagagat gtctagtgga
    34381 aaaactgagg tacaaacaga gtcagaggat acatctgcgg ctgatgcttt tttagcagat
    34441 tactatgcta aacgtaaagc aattgagaaa gaaatctcgt ctaattcgct gtcaacagat
    34501 gagtcagaat tttctgaggc acaggaagtt ttatcgcaat cacaggcaga cactataaaa
    34561 gctgagagtc aagaggaaaa atataatcgt agcctgaaaa aaacacgcac aggttttagt
    34621 gcacgcctta atgctttcct ttctaacttc cgtcgtgttg acgaagagtt ctttgaagaa
    34681 ttagaagaaa tgttaatctt atctgacgtt ggggtaaatg ttgcaacaca gttaaccgaa
    34741 gatcttcgtt atgaagcaaa attagagaat gccaagaaat cagaggattt aaaacgtgtt
    34801 atcgttgaaa aattagttga aatctacgaa aaagatggta tttataatga ggctatcaat
    34861 ttccaagaag gtttaaccgt aatgcttttt gtaggtgtta atggtgttgg aaaaacgaca
    34921 tctataggta agttagctca tcaatacaag tctcaaggga aaaaagttat gttagtagca
    34981 gctgatactt tccgtgcagg tgctgtggct caactagtag aatggggacg ccgtgttgat
    35041 gtaccagttg tcactggaga agaaaaagca gatccggcta gcgttgtctt tgatggcatg
    35101 gaaaaagccg ttgctcaagg cgttgatgtc ttgttaattg atacagcagg acgtctgcaa
    35161 aataaagaaa accttatggc agagttggaa aaaattggac gtattattaa gcgtgtcgta
    35221 cctgatgctc cccacgaaac tttgcttgct ttagatgcat ctacaggaca aaatgcttta
    35281 agtcaagcta aagagttctc gaaaattaca ccattaactg gccttattct gactaaaatt
    35341 gatggtactg ctaagggtgg tgtggtactg gctattcgtc aagaactaga tattcctgtt
    35401 aaattcattg gtttcggcga aaaaatcgat gatattggtg aatttaattc agaagatttc
    35461 atgcgtggcc ttctggaagg aattttataa gaaaaaagaa ctttcattta gaaagttctt
    35521 tttatattaa aagacacgat aaacataagg attattagct ttagaaactt gacgagactc
    35581 ttcaatgtgt aagactggta agttttgttg taacaattga ttatactcgg atttaattgt
    35641 acctctaaca gttacccaag tattgtccgg ataacttttt tgatttccag tcgttagtag
    35701 accataaact cctgaatcgg caatacaatg aatgattcca aagcggaaaa taaattgata
    35761 tccttcgtgt ttgggttcgt tataaacaaa cccagtatat tgtatttggc gatccatgaa
    35821 ttgctcggga tagagataaa ttagttccat aacttccatg taattttcag ttgtgatggt
    35881 taatgtacca gagcctttat attttttaag ctctttttgc atttcttttt cataggctga
    35941 agaagtaaaa taagtgctgg tatctggttt caaatattga acgcgtgtcc catcttgact
    36001 tacagtacca gtagagccgg ctgctagagg gaaattatat cccttggcag agactgtggt
    36061 tgagtctaaa ctaactgtag gtactaacaa acctactaaa acgggaaata ctaaaatcat
    36121 tgggcttgtt gatttagcta ttttaccatg taggtgacta tgcattttca tatttttaac
    36181 ccaaataatt agctgtacaa ttgctaaaat aaaagatagg accattgaaa tataggccaa
    36241 ataggtataa tgagtattaa tatactgatt tagtttacct gataacttta aatacatgct
    36301 taattcaaaa taaccagcaa gaataagaaa tcgaatcatt atattacccc cacaaataaa
    36361 caatagataa taataataag agaagataca ctaatgaatt ggacaataaa ccttgtttta
    36421 aaagaattta ccatcatcat taaattctta atatcaatca tgggtccaat taaaaggaaa
    36481 gccatcactg gcgcgattcc aaaggttgat aataaagaag cacctataaa agcgtcagct
    36541 tcactacaca atgacaagat gaaagcaagc aacatcataa ctaagatggc tgtgataggg
    36601 ctatgtccga tagtagtaag aactcgagtt ggtaaataaa tttgcatagc tgaagcaatt
    36661 aaagtaccaa atactagata gcgtcctgta tcaaaaaatt catcgatggc atgagctaag
    36721 gctagaaaga ttttttgata ccacttctta tcagaataat catgaaaatg agtaggttta
    36781 gcgtcttctt ttagaatatt atcatctact aaaaaggcaa gcatgactcc taaagcaatg
    36841 gctacaatgg tagcacctac aaagcgtaat atcaggaagc gtatggaatt tccaaatgct
    36901 gagtaggtcg caaataaaac aataggatta ataatagggg cagtagcaag aaacggaact
    36961 gccgtgtatg aaggcacttt cttttcaagg aatcgattaa taataggaat gataccacat
    37021 tcgcaagaag gaaagacaaa gccaacgaag gttccaaata agactctgag aaatttattt
    37081 ttcggtaaga acttattgac tatatcaggt gttataaaga cttcaataat tcctgataaa
    37141 atagttccaa gcaaaacaaa aggaagtgcc tcaataataa tggaaatgaa aatggcaaac
    37201 cattgaagga cactatccgg taattgatta aagatatcca tagaacttaa tctttctgag
    37261 aatcatcttt ctgttcaggt tttgaatcag aatcaacttt tttaggtttt ggtactttag
    37321 caaaactagc gaacatactt tctaagtcat cttgacgttt gtgcgtaata gccattaatg
    37381 gcacctcttt ctttatataa taacctatta tactatatta gcttattttt aacataaaaa
    37441 aacaaatcga ttggaaatga tactatatag tgataaaatg ttattagtag attgatgagg
    37501 taagattatg attgaattag gtattacaac attcggagag acaacaatcc ttgaagaaac
    37561 aaaccaaagc tattcacatc ctgagaggat tcgccaatta gttgctgaga ttgaactagc
    37621 tgatcaagtt ggtttagatg tatatggtat tggagagcac catcgtgaag attttgcggt
    37681 ctctgcaccc gaaattgtcc tagcagcagg agcggttaga actaataata tccgtttatc
    37741 tagtgcagta acgattctct cttccaatga tcctattcgc gtctaccagc aattttcaac
    37801 gattgacgca ctttcaaatg gtagagcaga aattatggca gggcgtggtt cctttattga
    37861 gtcttttcca ttgtttggat acgatttagc ggattatgat gatttattta atgaaaaaat
    37921 ggatatgttg ttagcaatta acttagcgac aaatctcgat tggaaaggtc atttgacaca
    37981 aacagttaat gagcgaccaa tttatccaag agcattacag agacagttac caatatgggt
    38041 ggcaacagga ggaaatgttg attctacaat tcgtattgca gaacaaggtt tgccaattgt
    38101 ttatgcaact attggtggga atcccaaagc atttcgtcaa ttggtccata tttataaaga
    38161 agttggtagt cgtaatggac acaaaccaga acaactaaaa gttgctgctc actcttgggg
    38221 atggattgaa gaggataatc aaaccgctat tgaccgttat tttttcccta cgaaacagac
    38281 cgtcgataat attgctaagg gacgccctca ttggtctgaa atgaccaaag agcagtattt
    38341 acgttcagta ggtccagaag gtgctatttt tgtaggaagt cctgaagtgg ttgcacataa
    38401 aattatagga cttgttgaag cacttgagtt agaccgtttt atgttacatc taccagttgg
    38461 ctctatgcct cacaaagacg tcctaaatgc tatcaaactt tatggtaagg aagtagcacc
    38521 aattgttcga aaatattttg aaaagaatta aagcagatct ggtctgtttt tttattgtta
    38581 gattataaaa acgctatctt gaaatcgctt tttaaaactc ttcgtaagtc aaaaatatgc
    38641 tataataaaa gcgttatgga aaatttaaat attacacaaa ttgctattga tttaggtatt
    38701 aaagcgtcac aaatcgaaaa ggttttagaa ttaacagatg aaggaaatac tattcctttt
    38761 attgcccgtt atcgtaaaga aatgacaggt aatttggatg aagttcaaat caaatcaatt
    38821 attgaccttg ataagagtat gacagctctt tcagatcgta aaactacggt cttagccaaa
    38881 attgaagaac aaggaaagtt gactcaagaa ctcaaaaagg ctattgaaga agcaactaaa
    38941 ctagcagatg ttgaggaact ctacctacct tataaagaaa aacgtcgtac gaaagctacg
    39001 attgcacgtg aagcaggtct ttttccttta gcacgtctta ttttacaaaa taaggataac
    39061 cttgaggaag aagctcaaaa ctatcttaca gatggttttg agacaactac gaaggcactt
    39121 tctggagcag tagatattct tatcgaagct ttttctgagg ataataaatt acgttcatgg
    39181 acatataatg agatctggaa ctacagctca atcactgcag ttgttaaaga tgagtcactt
    39241 gatgaaaaac aggtcttcaa aatctactat gacttttctg aaaaaataag taaactccat
    39301 ggctatcaag ttttagcatt gaatcgtggt gagaaaatgg gtgttcttaa ggttaatttt
    39361 gagcataacc ttgaaaaaat gtttcgcttt tttgctgttc gatttaaaga aactagccaa
    39421 tatatagatg accttattgt acagactgtc aagaaaaaaa tcgttccagc tatggaacgc
    39481 cgtatccgta cagaactaag tgaaggagca gaagatggtg caatttcact gttttcagag
    39541 aatcttcgta acttattgtt agtctcaccg cttaagggga aaatggtgtt aggttttgac
    39601 cctgcctttc gtacaggagc aaaattagca gtagtagacc aaactggaaa attaatgaca
    39661 acacaagtga tttatccagt tcctccagct aatcaagcaa aaatcgaaca atcaaaaata
    39721 gagttagcaa aacttatcaa agaattcaat atagaaatta ttgctatagg gaatggaaca
    39781 gcgagtcgtg aatcagaagc atttgttgca gaagttttac aagattttcc agacgtatcc
    39841 tatgtgattg ttaacgaaag tggtgcctct gtttattcag cctcagaatt ggcacgtcat
    39901 gaatttccag acttaacggt tgaaaaacgt tcggccatct caattgcaag acgtctgcaa
    39961 gacccattag cagaactagt caaaattgat ccaaaatcta ttggtgtggg tcaatatcaa
    40021 catgatgtta gccaaaaaaa attagcagaa aatcttgatt ttgttgttga aactgtagtt
    40081 aaccaagtag gtgttaatgt taatacagca agtccagcct tgttagctca tgtttcaggt
    40141 ttaaataaaa caatatctga aaatattgtt aaatatcgtg aagagaatgg gcaaattaag
    40201 tcacgtgctg aaattaagaa agtgcccaga ttaggagcaa aagcttttga gcaagctgct
    40261 ggattcttac gtattcctaa tgctaaaaat ttcttggata atacaggtgt tcaccctgag
    40321 tcttatgaag ctgttaaaaa gttgcttgac caactgacta tcaaagaatt agatgatctt
    40381 gctaaagaga agttacaaaa ccttgactta attgcaacag cagaaagtat tggggtaggt
    40441 caagaaacat taaaagacat tatagaagat cttttgaaac caggtcgtga tttgcgtgat
    40501 gattttgaag cgcctgtttt gagacatgat gttttagatg tcagtgattt aaaagttgga
    40561 caggaattgc aaggaactgt tcgaaacgtc gttgattttg gtgcttttgt tgatattggt
    40621 gttcatgaag atggtctaat ccaccagtct cgccttatta agcgaaaacg cgataaaaaa
    40681 actagaaaaa tgcctccttt acaacatcca agcaaatatc tctctgttgg tgatattgta
    40741 actgtttggg ttgtagaggt agatgcggag cgtagcagaa ttggacttag tcttatcaag
    40801 ccagacggac ttgactaatt atgttaagaa ggtgtctata gaagattttg gatgtcagtt
    40861 tttacatact gcacattgga ataatcgctt aagaacaact ggaggacgct tctttccaaa
    40921 cgatagacac cttgacttta atcccaaaat ttatcgtgag tttggtattg atatttttcg
    40981 taagattgta agacatgagc tttgccatta tcatctctat atacaaggta aaggatatca
    41041 acatagggat aaagctttta aagagttact agaaaaagtc gatggcttac gatatacccc
    41101 tagagttacg gctgttaaag tgaattatca tcattactct tgccaatctt gcggtcaagt
    41161 ataccgacgt aggagacgtg ttaatttacg caagtttgct tgtggttatt gtcatggtcg
    41221 cttgatagag agtttttaaa atcagtcgta aggcctattt aattttactg tcaaagtagt
    41281 tataattaaa atatagagta gatagaaaga aggataggta tgaaatcaag cttctacaaa
    41341 caacgaaaag gtaagttggt ctgcggtgtt gtcgccggtc tagctgataa gtataactgg
    41401 gatttagctc tttcacgggt gttgattgca ctgattttgt attttaccaa atttggtctt
    41461 ttgctatata ttttattagc tgttttctta ccctataaag aagatattat tgaaacaaga
    41521 agacaaggtc ccagaagacg taaagatgct gaacctgttg atgatgacgg ttggttctgg
    41581 taattcatat aaaggagatt tgctagatga atctcctttt ttcttagctt tatttagacc
    41641 actttttcaa aatatcttct ttttttcaga aaaacgcgtt agcgcttact tttagtaaaa
    41701 agtatgatat aatgtgggtg taaattaatg aaagtgttta gtacctttgc tcaactttaa
    41761 attgtagggt atagcaatag ggatattcac ttttataaga aggagtaagt atggcagtta
    41821 ctgttcaaat gttagttgac cgcttaaagt taaatgtcat ctatggtgat gaacatctat
    41881 tatctaagcg gattacaaca gctgacattt cacgtcctgg tctagaaatg acagggtatt
    41941 ttgattatta tgcaccagag cgtttgcaac ttgttggtat gaaagagtgg tcatatttaa
    42001 tggctatgac aggacataat cgttatcaag tgttacgtga aatgttccaa aaagaaacac
    42061 cagctattgt tgtagcacgt gatttagaaa ttccagaaga gatgtatgag gcggctaaag
    42121 ataccggtat tgctattctt caaagtaagg cccctacaag tcgtttatca ggtgaagttt
    42181 cttggtattt agattcttgc ttagctgaga ggacaagtgt tcatggtgtc ttgatggata
    42241 tctatggtat gggtgttctg attcaaggtg attccggtat tggtaagagt gagacaggac
    42301 tagagctagt taagcgagga catcgtttgg tagcagatga tcgtgttgat gtttatgcta
    42361 aagacgaaga gacgctttgg ggagagcctg ctgagatatt acgtcattta cttgaaatta
    42421 gaggtgtggg aatcattgat attatgagcc tatatggagc tagtgcggtt aaagattcat
    42481 cgcaagtgca attagctata taccttgaaa actttgaaac tggaaaagtt tttgatagac
    42541 tcgggaatgg caatgaagag atcgaattat ctggagttaa ggttcctcgg atacgtattc
    42601 ctgttaaaac aggccgaaac gtgtcagtag ttattgaagc agcggctatg aatcacagag
    42661 ctaaacaaat ggggtttgat gctacccaga cttttgagga tcgtttaaca catttgatta
    42721 gtcagaacga ggtcaatgat gattaatcca gtagcaatac gattaggccc cttttctatt
    42781 cgctggtatg ctatttgcat agtgtctggt atgttgttag cagtttactt agcaatgaaa
    42841 gaggcccctc gtaagaacat taagtcggat gatattctag atttcattct catggctttt
    42901 ccactatcca ttgttggagc tcgtatttat tatgttatct tcgaatgggc ttattactct
    42961 aagcacccag tagagattat cgccatctgg aatggcggta ttgctattta cggagggtta
    43021 attaccgggg ccattctcct tgtgattttt tcatatcgtc gcttgattaa tcccattgat
    43081 tttctggata ttgctgctcc aggagtgatg attgcccaag ccataggtag gtgggggaat
    43141 tttattaatc aagaagcata cggtagggca gtaaaaaatc tgaattacgt accaaacttt
    43201 ataaaaaatc aaatgtatat tgatggagct taccgagtac ctacattttt atatgagtct
    43261 ctttggaact tcctaggttt tgtcatcatc atgtctattc gtcatcgacc gcgcacttta
    43321 aaacaaggtg aagtggcatg tttttattta gtttggtatg gatgtggacg gttcattatt
    43381 gaagggatga ggacagatag cctttatttg gcgggtttgc gcgtgtcaca atggttatca
    43441 gttattttag ttattatcgg aattgtcatg attatttatc gtcgtcgtga acaacatatt
    43501 tcatattatt aaacagagga ggtattatga tagaaattgc tgtattaatt attgccattg
    43561 cgtttgttgt tttagtttta ggaatacttt tcgttctgaa aaaagtatca gaaacgattg
    43621 aggaaaccaa gcaaactatt aaagtattaa caagcgatgt taacgtgact ctttaccaaa
    43681 ctaatgaaat tttagccaaa gctaatgttt tagttgatga tgttaatggt aaagtatcta
    43741 ctattgatcc tctttttgta gctattgctg acttatcaga atctgtatct gatttaaacc
    43801 tacaggcaag acatattgga caaaaggcaa gtagtgcaac ctcaagtgtt actaaggcag
    43861 gttcagcttt agctattgga aaagcggctt caaaaatttt tcgtaaaaaa ggagacaaaa
    43921 aatgagtaaa ttatttaaaa ctttagttat cagtgctgca tcaggtgcag cagcagctta
    43981 ttttttaaca acaaaaaaag gaaaagagct aaggaaaaat gcagaaaaat tctatggaga
    44041 atataaagaa aatccagaag aatatcatca aatagctaaa gataaagcaa gtgaatattc
    44101 aaatttagct gttgatactt ttaaagatta taaaggtaaa tttgaatcag gtgaattgac
    44161 aacagaggat atcgtctcag ccgttaagga aaaaagcgga gaagtagttg actttgctaa
    44221 tgattttgtc aatcaagcta aatcaaaatt ctcagacgag gatactgcta aaaaagaaga
    44281 taaggctcct gaaacaaaag tagaagatat tgtcattgat tataaagaaa acacagaaga
    44341 taaagaaaaa taaaaaagaa gctactataa gtagcttctt ttttatgaga tttttattgt
    44401 ctgagtgacc aaatgaatga agagactgca gatgtaactg ctagacttgc cagcatcgct
    44461 agtaagaaac tccagacagg tgttaaactt agtgctagta ataagaaaga gattagcaca
    44521 gtggttatac tgaagagagc gatacgagca aaaaagacta gctcacgcaa cttagcactg
    44581 tgatcaaatt cttgaaatac ttgaaatttt ggttgagctt gctctgtttt gctttcgtga
    44641 gtttgctctt gcttaagtgg tgatgacata gctttctcct ttgatactag catatcatat
    44701 cacaaataat agagtttgtc actagttttt tgatactatg agttaaaata aaggtttttt
    44761 ctcaaaaa
//
LOCUS       DATRMA010000008        42529 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.008, whole genome shotgun sequence.
ACCESSION   DATRMA010000008 DATRMA010000000
VERSION     DATRMA010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 42529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42529
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.008"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            complement(237..602)
                     /gene="rplL"
                     /locus_tag="VBC28_000397"
     CDS             complement(237..602)
                     /gene="rplL"
                     /locus_tag="VBC28_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEO6814401.1"
                     /translation="MALNIENIIAEIKEATILELNDLVKAIEEEFGVTAAAPVAAAAA
                     GGEAAAAKDSFDVELTAAGDKKVGVIKVVREITGEGLKEAKAIVDNAPSVIKEGASEA
                     EANEIKEKLEAAGASVTLK"
     gene            complement(666..1166)
                     /locus_tag="VBC28_000398"
     CDS             complement(666..1166)
                     /locus_tag="VBC28_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEO6814402.1"
                     /translation="MSEAIIAKKAEQVELIAEKMKAAASIVVVDSRGLTVEQDTNLRR
                     SLRESDVEFKVIKNSILTRAAEKAGLEDLKELFVGPSAVAFSNEDVIAPAKVISDFAK
                     DAEALEIKGGSVDGKFTSVEEINALAKLPNKEGMLSMLLSVLQAPVRNVAYAVKAVAE
                     KDEEVA"
     misc_feature    complement(1196..1329)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            1599..3707
                     /locus_tag="VBC28_000399"
     CDS             1599..3707
                     /locus_tag="VBC28_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEO6814403.1"
                     /translation="MANNNFYNRDPFGNMDDIFNSLMGNMGGYNSENKRYLINGREVT
                     PEEFSQYRQTGKLPGQELNNQNTPTNQVSADSVLTKLGTNLTDQARQHLLDPVIGRNK
                     EIQETAEILARRTKNNPVLVGDAGVGKTAVIEGLAQAIINGDVPAAIKNKEIISIDIS
                     SLEAGTQYRGSFEENIQNIIKEVKETGNIILFFDEIHQILGAGSTGGDSGSKGLADIL
                     KPALSRGELTVIGATTQDEYRNTILKNAALARRFNEVKVNAPSAQDTFNILMGIRNLY
                     EQHHNVVLPDSVLKAAVDLSIQYIPQRSLPDKAIDLIDMTAAHLAAQHPVTDLKSLEK
                     EIAEQRDKQEKAVNTEDFEEALKVKTRIEELQNQIDNHTEGQKVTATINDIAMSIERL
                     TGVPVSNMGASDIERLKELGNRLKGKVIGQNDAVEAVARAIRRNRAGFDDGNRPIGSF
                     LFVGPTGVGKTELAKQLAFDMFGSKDAIVRLDMSEYNDRTAVSKLIGATAGYVGYDDN
                     SNTLTERIRRNPYSIVLLDEIEKADPQVITLLLQVLDDGRLTDGQGNTINFKNTVIIA
                     TSNAGFGNEAFTGDSDKDLKIMERISPYFRPEFLNRFNGVIEFSHLSKDDLSEIVDLM
                     LDEVNQTISKKGIDLVVDENVKSHLIELGYDEAMGVRPLRRVIEQEIRDRITDYYLDH
                     TDVKHLKANLQDGQIVISER"
     gene            complement(3800..4744)
                     /gene="mmuM"
                     /locus_tag="VBC28_000400"
     CDS             complement(3800..4744)
                     /gene="mmuM"
                     /locus_tag="VBC28_000400"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000532339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase"
                     /protein_id="HEO6814404.1"
                     /translation="MGRFKELLESKKTLILHGALGTELESRGCDVSGKLWSAKYLIED
                     PAAIQTIHEDYIRAGADIVTTSTYQATLQGLAQVGVSESQAEDLIRLTVQLAKAAREQ
                     VWKSLTKEEKSERIYPLISGDVGPYAAFLADGSEYTGLYDIDKQGLKNFHRHRIELLL
                     DEGVDILALETIPNAQEAEALIELLAEDFPQVEAYMSFTSQDGKTISDGSAVADLAKA
                     IDVSPQVVALGINCSSPSLVADFLQAIAEQTNKPLMTYPNSGEVYDGASQSWQSSPDH
                     SHTLLENTSDWQKLGAQVVGGCCRTRPADIADLSAHLK"
     gene            complement(4749..6125)
                     /locus_tag="VBC28_000401"
                     /pseudo
     CDS             complement(4749..6125)
                     /locus_tag="VBC28_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058615.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
     gene            complement(6255..6905)
                     /locus_tag="VBC28_000402"
     CDS             complement(6255..6905)
                     /locus_tag="VBC28_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HXXEE domain-containing protein"
                     /protein_id="HEO6814405.1"
                     /translation="MSYFRNYWYRFGAILFIILAVILLVFRPDWSMLHYLLYFNFMAL
                     LAHQFEEYQFPGGASPIINYVVYDEEELMDCFPGNTQSIMLVNTIAWLLYIASIAFPQ
                     AYWLGLGVMFFSLTQLLGHGFQMNIKLKTWYNPGLATTVFLLVPIACAYIYQASAEGM
                     LTWGDWLGGFIMLIVCVLTSIIAPVQLLKDKETNYIISPWQMDRFHKVVNFVRIKK"
     gene            complement(6917..7566)
                     /locus_tag="VBC28_000403"
                     /pseudo
     CDS             complement(6917..7566)
                     /locus_tag="VBC28_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000645479.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HXXEE domain-containing protein"
     gene            7658..8206
                     /locus_tag="VBC28_000404"
     CDS             7658..8206
                     /locus_tag="VBC28_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEO6814406.1"
                     /translation="MTESVTVNKKRIKQITQTQEDILTAFGQLLEEYPYEKIQISQIA
                     KKSGYARRTFYRHFDSRDDLLTLFIERLTLNLFKQLGQLEQPTFSQVFQNFFSYWSDY
                     KSLLLILRKNDLLPQFQQSWFRHIDLIELGRGDLSSNTYAQRFAIGGIFSVLIEWIHQ
                     DCQTSIEELTQVSFDIINHLKN"
     gene            complement(8268..8864)
                     /locus_tag="VBC28_000405"
     CDS             complement(8268..8864)
                     /locus_tag="VBC28_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000796923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HEO6814407.1"
                     /translation="MAEEFLNTHNASILLSAANKSHYPQDDLPEVALAGRSNVGKSSF
                     INTLLGRKNLARTSSKPGKTQLLNFYNIDDKLRFVDVPGYGYAKVSKTERAKWGKMIE
                     EYLVTRDNLRVVVSLVDFRHDPSADDIQMYEFLKYYEIPVIIVATKADKIPRGKWNKH
                     ESSIKKKLNFDKKDHFIVFSSVDRTGLDESWDTILSEL"
     gene            complement(8875..10101)
                     /gene="clpX"
                     /locus_tag="VBC28_000406"
     CDS             complement(8875..10101)
                     /gene="clpX"
                     /locus_tag="VBC28_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEO6814408.1"
                     /translation="MAGNRNNDMNVYCSFCGKSQDEVKKIIAGNGVFICNECVALSQE
                     IIKEELAEEVLADLAEVPKPKELLEILNQYVVGQDRAKRALAVAVYNHYKRVSYTESS
                     DDDVDLQKSNILMIGPTGSGKTFLAQTLAKSLNVPFAIADATSLTEAGYVGEDVENIL
                     LKLIQAADYNVERAERGIIYVDEIDKIAKKGENVSITRDVSGEGVQQALLKIIEGTVA
                     SVPPQGGRKHPNQEMIQINTKNILFIVGGAFDGIEDLVKQRLGEKVIGFGQTSRKIDD
                     NASYMQEIISEDIQKFGLIPEFIGRLPVVAALELLTAEDLVRILTEPRNALVKQYQTL
                     LSYDGVELEFDQDALLAIADKAIERKTGARGLRSIIEETMLDIMFEIPSQEDVTKVRI
                     TKAAVEGTDKPVLETA"
     gene            complement(10319..10813)
                     /locus_tag="VBC28_000407"
     CDS             complement(10319..10813)
                     /locus_tag="VBC28_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEO6814409.1"
                     /translation="MTKQIIAIWAEDEDHLIGVNGGLPWRLPKELHHFKETTMGQALL
                     MGRKTFDGMNRRVLPGRETIILTKDEQFQADGVTVLNSVEQVIKWFQEHNKTLFIVGG
                     ASIYKAFLPYCEAIIKTKVHGKFKGDTYFPDVNLSEFKVISRDYFEKDEQNAHAFTVT
                     YFEK"
     gene            complement(10893..11732)
                     /locus_tag="VBC28_000408"
     CDS             complement(10893..11732)
                     /locus_tag="VBC28_000408"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEO6814410.1"
                     /translation="MTKADLLFKDNITKIMSEGVFSEQARPRYKNGEMANSKYITGAF
                     AEYDLSKGEFPITTLRPIPIKSAIKEIFWIYQDQTNDLAVLNDKYGVTYWNDWEVGHT
                     GTIGQRYGAVVKKHNIISKLLKQLEDNPWNRRNVISLWDYEAFEETEGLLPCAFQTMF
                     DVRRVNGELYLDATLTQRSNDMLVAHHINAMQYVALQMMIAKHFGWRVGKFFYFINNL
                     HIYDNQFEQAQELLKRQPSECNPKLVLNVPDGTDFFDIKPDDFALVDYDPIKPQLRFD
                     LAI"
     gene            11781..13058
                     /locus_tag="VBC28_000409"
     CDS             11781..13058
                     /locus_tag="VBC28_000409"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HEO6814411.1"
                     /translation="MRNKTLKVKAFFHIFSFFMVKWNLAKMTFIQKGLPMKIGIDKIG
                     FATSQYVLEMTDLAIARQVDPEKFSKGLLLDSLSITPVTEDIVTLAASAANDILSDED
                     KETIDMVIVATESSIDQSKAASVYVHQLLEIQPFARSFEMKEACYSATAALDYAKLHV
                     EKHPDSKVLVIASDIAKYGIKSTGESTQGAGSIAMLISQNPSILELKEDHLAQTRDIM
                     DFWRPNYSDVPYVNGMFSTKQYLDMLKTTWKEYQKRFNTSLSDYAAFCFHIPFPKLAL
                     KGFNKILDNNLDEQKKAELQENFEHSITYSKKIGNCYTGSLYLGLLSLLENSQNLKAG
                     DQIAFFSYGSGAVAEIFTGQLVDGYQNKLQSDRMDQLNKRQKITVTEYEKLFFEKTIL
                     DENGNANFNTYRTGTFSLDSICEHQRIYKKINN"
     gene            13060..14343
                     /locus_tag="VBC28_000410"
     CDS             13060..14343
                     /locus_tag="VBC28_000410"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HEO6814412.1"
                     /translation="MTKISWTGFSKKSPEERIHYLEEQDFLADSSLEIVTNQDLLSLS
                     LANQMAENVIGRIALPFSLVPDVLVNGKVYQVPYVTEEPSVVAAASFAAKIIKRSGGF
                     LTTVHNRKMIGQVAIYDVQDSQHTKESILNQKQQLLEIANAAHPSIVKRGGGACDLTI
                     EIKEDFLIVYLMVDTKEAMGANMVNTMMEALSSPLEDISKGKSLMSILSNYATESLVT
                     ATCRVDLRFLSRQKEEAIKLAQKMTMASQLAQVDPYRASTHNKGIFNGIDAIVLATGN
                     DWRAIEAGAHTYAVKDGQYRGLSRWSYKVDDNCLEGTLTLPMPVATKGGSIGINPSVH
                     LAHDLLGRPNAKELASIILSIGLAQNFAALKALVSTGIQAGHMKLQAKSLALLAGAKE
                     EQISEVVKQLLDSKHMNLETAQKIVNKLTKSKKRI"
     gene            14435..14884
                     /locus_tag="VBC28_000411"
     CDS             14435..14884
                     /locus_tag="VBC28_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001039194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HEO6814413.1"
                     /translation="MNKNQYPKWEELPELDLYLDQVLLYVNQLINPKTITNDKLLTAS
                     MINNYVKHNYISKPIKKKYNRRQVARLIVITAFKQVFAIQEISQTLELLTADNHSEEA
                     YNGFAACMNKEEVHDLPPVVISACQTLNLYQETQKLVLELGGKNKNA"
     gene            14877..15533
                     /locus_tag="VBC28_000412"
     CDS             14877..15533
                     /locus_tag="VBC28_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000910676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEO6814414.1"
                     /translation="MLKEMSIKLSPQLSFGEELANSITHAVGALLMLILLPITAVYSH
                     NHFGLQAALGTSIFVTSLFLMFLSSSIYHSMTYNSLQKYVLRMIDHSMIYIAIAGSYT
                     PVALSLIGGWLGYLIIFLQWGITLFGILYKIFAPKINDKFSLVLYLIMGWLVIFIFPA
                     IITKTGPAFWGLLLAGGICYTIGALFYARKRPYDHMIWHLFILLASILQYIGIVYFML
                     "
     gene            15620..16534
                     /locus_tag="VBC28_000413"
     CDS             15620..16534
                     /locus_tag="VBC28_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEO6814415.1"
                     /translation="MKSAYIFFNPKSGKDEQALAQEVKSYLIEHDFQDDYVRIITPSS
                     VEEAVALAKKASEDHIDLVIPLGGDGTINKICGGVYAGGAYPTIGLVPAGTVNNFSKA
                     LNIPQERNLALENLLNGHVKSVDICKVNDDYMISSLTLGLLADIAANVTSEMKRKLGP
                     FAFLGDAYRILKRNRSYSITLAYDNNVRSLRTRLLLITMTNSIAGMPAFSPEATIDDG
                     LFRVYTMEHIHFFKLLLHLRQFRKGDFSQAKEIKHFHTNNLTISTFKRKKSAIPKVRI
                     DGDPGDQLPVKVEVIPKALKFIIPNSLP"
     gene            16635..17489
                     /locus_tag="VBC28_000414"
     CDS             16635..17489
                     /locus_tag="VBC28_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEO6814416.1"
                     /translation="MTTFTAKFIDQEWEVPVESGRYHMIVGEFCPYAQRPQIARQLLG
                     LDKHISISFVDDVPSDIGLIFSQPEQVTGAKSLRDIYHLTDPTYQGPYTIPILIDKTD
                     NRIVCKESADLLRLFTTDFSDLHQEDAPVLFSQETASLIDNDIKDINKNFQSLMYKLA
                     FLDKQADYDTYSKEFFTFLDQKEHLLGQRPFLLGDNLSEVDIHFFTPLVRWDIAGRDL
                     LLLNQKALEDYPNIFSWAKTLYNDFNLKTLTNPQSIKNNYYLGKFGRAVRHHTIVPTG
                     PNMAKWEK"
     gene            complement(17535..17753)
                     /locus_tag="VBC28_000415"
     CDS             complement(17535..17753)
                     /locus_tag="VBC28_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HEO6814417.1"
                     /translation="MIKLYYKDAYDTERCQVFENPNDFIRVLMGCVTVPDNYPITKMT
                     YKDKDIAYSGTYGDLFKTFIEFDWSTLD"
     gene            complement(17821..18816)
                     /locus_tag="VBC28_000416"
     CDS             complement(17821..18816)
                     /locus_tag="VBC28_000416"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HEO6814418.1"
                     /translation="MTNRKDDHIKYALKYQSPYNSFDDIELIHSSLPKYNVNDIDLST
                     HFAGQSFEFPFYINAMTGGSEKGKAVNHKLAQVAQATGIVMVTGSYSAALKNDEDDSY
                     PTTDLYPDLKLATNIGLDKPVPAAESTVKAMNPIFLQVHVNVMQELLMPEGEREFHMW
                     RSHLKEYVDNIQCPLILKEVGFGMDLQSIKDAYDIGITTVDISGRGGTSFAYIENQRG
                     RDRSYLNTWGQTTAQSLINAQSMMDKMDILASGGIRHPLDMVKCLVLGAKAVGLSRTV
                     LELVERYPVDDVIAILNSWKEDLRMIMCALNCKKITDLRQVNYILYGQLKEANAK"
     gene            complement(18813..19805)
                     /locus_tag="VBC28_000417"
     CDS             complement(18813..19805)
                     /locus_tag="VBC28_000417"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HEO6814419.1"
                     /translation="MVKVQTGGKLYIAGEYAILYPGQVAILKNVPIYMTALATFADNY
                     SLYSDMFNYTASLQPDKQYSLIQETILLMEEWLINFGKNIKPIHLEITGKLERYGLKF
                     GIGSSGSVVVLTIKAMAALYEIEMPSDLLFKLSAYVLLKRGDNGSMGDIACIAYEHLI
                     SYSAFDRRAVSKMIETKPLEQVLEAEWGYRITKIQASLEMDFLVGWTMQPSISKEMIN
                     IVKSTITQRFLDDTKYQVVQLLSAFKEGDKEAIKRCLEEISLLLFNLHPSIYTDKLQK
                     LKEASKGLDIVTKSSGSGGGDCGIAISFNKNDNQTLIKRWESAGIELLSKETLS"
     gene            complement(19798..20742)
                     /gene="mvaD"
                     /locus_tag="VBC28_000418"
     CDS             complement(19798..20742)
                     /gene="mvaD"
                     /locus_tag="VBC28_000418"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000348814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HEO6814420.1"
                     /translation="MDGKSISVKSYANIAIIKYWGKADAEKMIPATSSISLTLENMYT
                     ETRLTALGKDAKKDEFYISGVLQNDHEHDKMSAILDRFRQNKSGFVKIETTNNMPTAA
                     GLSSSSSGLSALVKACNDFFGTNLSQSQLAQEAKFASGSSSRSFFGPVAAWDKDSGDI
                     YKVHTNLDLAMIMLVLNDKRKPISSREGMKICTETSTTFNEWVRQSEQDYQDMLVYLK
                     NNDFQKVGQLTERNALAMHSTTKTATPAFSYLTEETYKAMDVVKKLREKGHECYYTMD
                     AGPNVKVLCLRQDLEALAAILEKDYRIIVSTTKELADG"
     gene            complement(20724..21602)
                     /gene="mvk"
                     /locus_tag="VBC28_000419"
     CDS             complement(20724..21602)
                     /gene="mvk"
                     /locus_tag="VBC28_000419"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HEO6814421.1"
                     /translation="MKEKFGIGKAHSKIILMGEHSVVYGYPAIAIPLKNIEVTCLIEE
                     APQLIALDMTDPLSTAIFAALDYLGKTSSKIAYHIESQVPERRGMGSSAAVAIAAIRA
                     VFDYFDEDLEADLLECLVNRAEMIAHSNPSGLDAKTCLSENTIKFIRNIGFSTVPMHL
                     NAYLVIADTGIHGHTKEAVDKVKSSGEAVLPFLKELGYLAEASEDAIHKSDSKQLGSL
                     MTKAHQSLKQLGVSSLEADHLVEVAISCGALGAKMSGGGLGGCIIALVKEKREAERLS
                     QQLEREGAVNTWTEKV"
     gene            complement(21718..22947)
                     /locus_tag="VBC28_000420"
     CDS             complement(21718..22947)
                     /locus_tag="VBC28_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEO6814422.1"
                     /translation="MFRNLRLRFIGIAALAILVVLFSVVGVLNSANHYQTKNEIYRVL
                     TILADNNGRIPNKLEFSKELGDDLSTDAIFQFRYFSARTDAKGNVTSFDSRNIFEVSD
                     RQIKNYAKRIVSQNSHSGHITYNFSTYSYLLKKVGKNDYLVVFLDTTNQYLDNQRLLQ
                     LSIWMSLVSFIVFMVIVSVLSGRVILPFVANYEKQRRFITNAGHELKTPLAIISANNE
                     LVEMMSGESEWTKSTNDQIQRLTGLINGMVSLARFEEQPDISMVDLDFSHITKDAAED
                     FKGPIIKDGKDFIMSIQPGIHVKAEEKSLFELVTLLVDNANKYCDPMGTVTVKLSRSS
                     RLRRAKLEVSNTYKNGKDIDYSKFFERFYREDESHNNKKSGYGIGLSIVTSLVHLFKG
                     SIDVNYKHDTITFVIYI"
     gene            complement(22947..23633)
                     /locus_tag="VBC28_000421"
     CDS             complement(22947..23633)
                     /locus_tag="VBC28_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO6814423.1"
                     /translation="MRVLIAEDEEQMSRVLSTAISHQGYVVDVAYDGQTAIDLANQNA
                     YDVMVMDVMMPVKTGVEAVKEIRQSGNKSHIIMLTAMAEIDDRVTGLDAGADDYLTKP
                     FSLKELLARLRSMSRRLEDFTPNVLSLGRVTLSVGEQELQCENTIRLAGKEAKMLAFF
                     MLNHDKELSTQQLFEHVWGADKDQEDVDEGYVFIYVSYLRQKLQAIGANLEIIGQEGS
                     SYKLSEINGG"
     gene            complement(23635..24261)
                     /locus_tag="VBC28_000422"
     CDS             complement(23635..24261)
                     /locus_tag="VBC28_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000449638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HEO6814424.1"
                     /translation="METNIYGDYGRYLPLILEDFSQRIQLENDKAKVETGYKLYEHII
                     GRIKTSDSMIEKCRRKQLPVTVDSALKVIRDSIGVRIICGFVNDIYQIVERIKTFDDC
                     RIVVEKDYIQHVKPNGYRSYHVILEIDTPYPDCLGNSEGKYYMEIQLRTIAQDSWASL
                     EHQMKYKHDIENPERIVRELKRCADEIASVDLTMQTIRQLIESGTKKE"
     gene            complement(24369..26123)
                     /locus_tag="VBC28_000423"
     CDS             complement(24369..26123)
                     /locus_tag="VBC28_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000851082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6814425.1"
                     /translation="MKTKSQKTSFFSNLSPYLKGYKLLFALAIVLATLSSLTTVIGPD
                     RLKEMTDLMVKGLAGNIDLAKIGHIAGQLAVLYVLGALVSYLASFIVTTIIQKFAQGM
                     RNAIADKINKIPLSYFDSHTQGDTLSRVTNDVDLMTQSFNQSLVTMVASTVLLVGSLI
                     MMFKTDWHLATTAIISVFSGFALSSIIMVKSQPLFKKQQVNLANISGYVEEIYSGHNI
                     VTSHNARQQAKSDFQLLNDKLHSSMWKSQFFSGIMMPLMQFIGNFGYVMVCVVGAMLA
                     IKGTITMGTIVAFMTYVRIFTQPIGQIAQGITQLQSASAAMGRVFEFLNETNMQEDKH
                     KVLNFPKFKGHVSFEHVKFGYSDNKTIIHDFTSEAQPGQKVAIVGPTGAGKTTIVNLL
                     MRFYDVNSGSISIDDINIQDMKRSQVHDAFSMVLQDTWLFEGTIKENLIFNQTTISDD
                     EVQEAAKAVGIHHFIMTLPKGYDTVLDDAVTLSVGQKQLLTIARALLKNSPLLILDEA
                     TSSVDTRTEELIQKAMDKLMEGRTSFVIAHRLSTIKNADLILVMKDGNIIEQGNHDVL
                     MSQSGFYADLYNSQFESA"
     gene            complement(26113..27930)
                     /locus_tag="VBC28_000424"
     CDS             complement(26113..27930)
                     /locus_tag="VBC28_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6814426.1"
                     /translation="MFNIFKRLNGKEWLMIVASTILVCLAVWMDLKTPEYMSDITRML
                     QTKGTKLEALWDPGLKMLAFSFGSFLVAVMVGFLGARTAASFTSRLRSDIFNHVMDYS
                     DAEIKRFSIPSLLTRTTNDLTQLQILIVMGMQVVTRGPIMAIWALTKIWGKSDQWTGA
                     AGVAVIIVMILLSVLLLLAFPRQRQIQGFTDALNATTREALSGVRVIRAYNAEKYQTD
                     KFKRENDDLTKLNLFVYRLMSLMNPVMTVVSSGLTLAIYWIGSYLINDISVPKVTGTS
                     VSVLAAKSAITDRVDLFSNMVVFSSYAMQVVMGFMMMVAIFIIMPRALVSARRINEVL
                     TLAPTVTFKENSHIQSAPKGQIEFQDVSFHYSKDSEAVIEHVSFKADTGQTVAFIGST
                     GSGKSTLVNLVPRFYDATEGSIKLDGIDIKEYSHNDLNNKIGYIPQKAVLFSGTVRSN
                     MIFGESGREELSDEQIWQALELAQAKEFIEAKEKGLDTEVAQGGSNFSGGQKQRLAIA
                     RALARHPEILIFDDSFSALDYKTDHILRHVLKEKTAAMTKLIVAQRISTIMDADLILV
                     LDQGKVVGQGTHKELLSNNEVYQEIAYSQLSKEELENEN"
     gene            complement(27977..28417)
                     /locus_tag="VBC28_000425"
     CDS             complement(27977..28417)
                     /locus_tag="VBC28_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEO6814427.1"
                     /translation="MENPLQKARILVNQLEKYLDRYAKEYDVEHLAGPQGHLVMHLYK
                     HPDKDMSIKDAEEILHISKSVASNLVKRMEKNGFIAIVPSKTDKRVKYLYLTHLGKQK
                     ATQFEIFLEKLHSTMLAGITKEEIRTTKKVIRTLAKNMAMEDFD"
     gene            28686..30758
                     /locus_tag="VBC28_000426"
     CDS             28686..30758
                     /locus_tag="VBC28_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HEO6814428.1"
                     /translation="MKKKIILKSSVLGLVAGTSIMFSSVFADQVGVQVIGVNDFHGAL
                     DNTGTANMPDGKVTNAGTAAQLDAYMDDAQKDFKQTNPNGESIRVQAGDMVGASPANS
                     GLLQDEPTVKTFNAKNVEYGTLGNHEFDEGLAEYNRIVTGKAPAPDSNINNITKSYPH
                     EAAKQEIVVANVIDKVNKQIPYNWKPYTIKNIPVNNKSVNVGFIGIVTKDIPNLVLRK
                     NYEQYEFLDEAETIVKYAKELQAKNVKAIVVLAHVPATSKDDIAEGEAAEMMKKVNQL
                     FPENSVDIVFAGHNHQYTNGLVGKTRIVQALSQGKAYADVRGVLDTDTQDFIETPSAK
                     VIAVAPGKKTGSADIQAIVDQANTIVKQVTEAKIGTAEVSGMITRSVDQDNVSPVGSL
                     ITEAQLAIARKSWPDIDFAMTNNGGIRADLLIKPDGTITWGAAQAVQPFGNILQVVEI
                     TGRDLYKALNEQYDQKQNFFLQIAGLRYTYTDNKEGGEETPFKVVKAYKSNGEEINPD
                     AKYKLVINDFLFGGGDGFASFRNAKLLGAINPDTEVFMAYITDLEKAGKKVSVPNNKP
                     KIYVTMKMVNETITQNDGTYSIIKKLYLDRQGNIVAQEIVSDTLNQTKSKSTKINPVT
                     TIHKKQLHQFTAINPMRNYGKPSNSTTVKSKQLPKTNSEYGQSFLMSVFGVGLIGIAL
                     NTKKKHMK"
     gene            complement(30795..31205)
                     /locus_tag="VBC28_000427"
     CDS             complement(30795..31205)
                     /locus_tag="VBC28_000427"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEO6814429.1"
                     /translation="MIKPIVRDTFFLQQKSQMASRADVSLAKDLQDTLHANQNYCVGM
                     AANMIGSLKRVIIINVGITNLVMFNPVLVAKSDPYETEESCLSLVGCRSTQRYHHITI
                     SYRDINWKEQQIKLTDFPAQICQHELDHLEGILI"
     gene            complement(31275..32624)
                     /gene="gdhA"
                     /locus_tag="VBC28_000428"
     CDS             complement(31275..32624)
                     /gene="gdhA"
                     /locus_tag="VBC28_000428"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HEO6814430.1"
                     /translation="MTSGKTYVASVLEKVKKQNEHEEEFLQAVEEVFESLVPVFDKYP
                     QYIEENLLERLVEPERVISFRVPWVDDKGQVQVNRGYRVQFSSAIGPYKGGLRFHPTV
                     TQSIVKFLGFEQIFKNSLTGLPIGGGKGGSNFDPKGKSDNEVMRFTQSFMTELQKYIG
                     PDLDVPAGDIGVGGREIGYLYGQYKRLNGYQNGVLTGKGLTYGGSLARTEATGYGAVY
                     FAKEMLAACGQDLTGKVALVSGSGNVAIYATEKLQELGATVVAVSDSSGYVYDPDGID
                     LETLKQIKEVERARIVKYTEKHPKANFTPADQGSIWSIKADLAFPCATQNELDEEDAK
                     LLVENGVLAVTEGANMPSTLGAIKVFQKAGVAFGPAKAANAGGVAVSALEMAQNSSRR
                     AWTFEEVDQELQRIMKTIFVNASKAADEFGDSGNLVLGANIAGFLKVAQAMSAQGIV"
     gene            32792..33301
                     /locus_tag="VBC28_000429"
     CDS             32792..33301
                     /locus_tag="VBC28_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HEO6814431.1"
                     /translation="MKWFPLVGGIIALLAGFYLFINPVTVVATLGWFIALFIFIYGIS
                     NLVRYLQADSYHRSPWYLVQSILAIIFGIILLSSSAMSLSNTVMTILAYWLLVSGILR
                     LLGGFQMQKINFPGAKSYKRSAYIALILGIILLVQPTLSAAIIGRFVAFILIAIGASA
                     IYTFTRINY"
     gene            complement(33342..35111)
                     /locus_tag="VBC28_000430"
     CDS             complement(33342..35111)
                     /locus_tag="VBC28_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6814432.1"
                     /translation="MRKKSVFLRLWSYLTRYKATLFLAIFLKVLSSFMSVLEPFILGL
                     AITELTANLVDMAKGVSGAELNVPYIAGILIIYFFRGVFYELGSYGSNYFMTTVVQKS
                     IRDIRHDLNHKINKVPVSYFDKHQFGDMLGRFTSDVETVSNALQQSFLQIINAFLSII
                     LVVVMVLYLNVPLAMIIIACIPVTYFSAQAILKRSQPYFKEQAKILGELNGFVQEKLT
                     GFNIIKLYGREEASSQEFRDITDNLRHVGFKASFISGIMMPVLNGISDFIYLIIAFVG
                     GLQVIAGTLTIGNMQAFVQYVWQISQPVQTITQLSGVLQSAKSSLERIFEVLDEEEEA
                     NQVTEKLSHDLTGQVSFHGVDFHYSPDRPLIRDFNLDVEPGQMIAIVGPTGAGKTTLI
                     NLLMRFYDVSEGAITVDGHDIRHLSRQDFRQQFGMVLQDAWLYEGTIKENLRFGNLEA
                     SDEDIVAAAKAANVDHFIRTLPGGYNMVMNQESSNISLGQKQLLTIARALLADPKILI
                     LDEATSSVDTRLELLIQKAMKKLMEGRTSFVIAHRLSTIQEADNILVLKDGQIIEQGN
                     HQKLLADKGFYYELYNSQFSNSK"
     gene            complement(35116..36855)
                     /locus_tag="VBC28_000431"
     CDS             complement(35116..36855)
                     /locus_tag="VBC28_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6814433.1"
                     /translation="MAYIWSYLKRYPNWLWLDLLGAMLFVTVILGMPTALAGMIDNGV
                     TKGDRTGVYLWTFIMFIFVVLGIIGRITMAYASSRLTTTMIRDMRNDMYAKLQEYSHH
                     EYEQIGVSSLVTRMTSDTFVLMQFAEMSLRLGLVTPMVMIFSVVMILITSPSLAWLVA
                     VAMPLLVGVVLYVAIKTKPLSERQQTMLDKINQYVRENLTGLRVVRAFARENFQSQKF
                     QVANQRYTDTSTGLFKLTGLTEPLFVQIIIAMIVAIVWFALDPLQRGAIKIGDLVAFI
                     EYSFHALFSFLLFANLFTMYPRMVVSSHRIREVMDMPISINPNAEGVTDTKLKGHLEF
                     DNVTFAYPGETESPVLHDISFKAKPGETIAFIGSTGSGKSSLVNLIPRFYDVTLGKIL
                     VDGVDVRDYNLKSLRQKIGFIPQKALLFTGTIGENLKYGKADATIDDLRQAVDISQAK
                     EFIESHQEAFETHLAEGGSNLSGGQKQRLSIARAVVKDPDLYIFDDSFSALDYKTDAT
                     LRARLKEVTGDSTVLIVAQRVGTIMDADQIIVLDEGEIVGRGTHAQLIENNAIYREIA
                     ESQLKNQNLSEGE"
     gene            complement(36922..37395)
                     /locus_tag="VBC28_000432"
     CDS             complement(36922..37395)
                     /locus_tag="VBC28_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO6814434.1"
                     /translation="MIIRPIIKNDDQAVAQLIRQSLRAYDLDKPDTAYSDPHLDHLTS
                     YYEKIEKSGFFVIEERDEIIGCGGFGPLKNLIAEMQKVYIAERFRGKGLATDLVKMIE
                     VEARKIGYRQLYLETASTLSRATAVYKHMGYCALSQPIANDQGHTAMDIWMIKDL"
     gene            complement(37392..39260)
                     /locus_tag="VBC28_000433"
     CDS             complement(37392..39260)
                     /locus_tag="VBC28_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEO6814435.1"
                     /translation="MSDFLVDGLTKSVGDKTVFSNVSFIIHSLDRIGIIGVNGTGKTT
                     LLDVISGELGFDGDRSPFSSANDYKIAYLKQEPDFDDSQTILDTVLSSDLREMALIKE
                     YELLLNHYEESKQSRLEKVMAEMDSLDAWSIESEVKTVLSKLGITDLQLSVGELSGGL
                     RRRVQLAQVLLNDADLLLLDEPTNHLDIDTIAWLTNFLKNSKKTVLFITHDRYFLDNV
                     ATRIFELDKAQITEYQGNYQDYVRLRAEQDERDAASLHKKKQLYKQELAWMRTQPQAR
                     ATKQQARINRFQNLKNDLHQTSDTSDLEMTFETSRIGKKVINFENVSFSYPDKSILKD
                     FNLLIQNKDRIGIVGDNGVGKSTLLNLIVQDLQPDSGNVSIGETIRVGYFSQQLHNMD
                     GSKRVINYLQEVADEVKTSVGTTSVTELLEQFLFPRSTHGTQIAKLSGGEKKRLYLLK
                     ILIEKPNVLLLDEPTNDLDIATLTVLENFLQGFGGPVITVSHDRYFLDKVANKIIAFE
                     DNDIREFFGNYTDYLDEKAFNEQNNEVISKKESTKTSREKQSRKRMSYFEKQEWATIE
                     DDIMILENTITRIENDMQTCGSDFTRLSDLQKELDAKNEALLEKYDRYEYLSELDT"
     gene            complement(39271..40479)
                     /locus_tag="VBC28_000434"
     CDS             complement(39271..40479)
                     /locus_tag="VBC28_000434"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEO6814436.1"
                     /translation="MRLNYLPSEFQKALPILKKIKKAGYEAYFVGGSVRDVLLDRPIH
                     DVDIATSSYPEETKQIFKRTVDVGIEHGTVLVLEKGGEYEITTFRTEEVYVDYRRPSQ
                     VNFVRSLEEDLKRRDFTVNAFALNEDGEVIDLFHGLDDLDNHLLRAVGLASERFNEDA
                     LRIMRGLRFSASLNFDIETTTFEAMKKHASLLEKISVERSFIEFDKLLLAPYWRKGML
                     ALIDSHAFNYLPCLKNRELQLSAFLSQLDKDFLFETSEQAWASLILSMEVEHTKTFLK
                     KWKTSTHFQKDVEHIVDVYRIREQMGLTKEHLYRYGKTIIKQAEGIRKARGLMVDFEK
                     IEQLDSELAIHDRHEIVVNGGTLIKKLGIKPGPQMGDIISQIELAIVLGQLINEEEAI
                     LHFIKQYLMD"
     gene            complement(40664..41512)
                     /locus_tag="VBC28_000435"
     CDS             complement(40664..41512)
                     /locus_tag="VBC28_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEO6814437.1"
                     /translation="MKLALITDTSAYLPEAIENHEDVYVLDIPIIIDGKTYIEGQNLT
                     LDQYYDKLAASKELPKTSQPSLAELDDLLCQLEKEGYTHVLGLFIAAGISGFWQNIQF
                     LIEEHPNLTIAFPDTKITSAPQGNLVRNALMCSREGMDFDVIVNKIQSQIEKIEGFIV
                     VNDLNHLVKGGRLSNGSAIIGNLLSIKPVLHFNEEGKIVVYEKVRTEKKALKRLAEIV
                     KEMTADGEYDIAIIHSRAQDKAEQLYNLLAKAGLKDDLEIVSFGGVIATHLGEGAVAF
                     GITPKN"
     gene            complement(41512..41892)
                     /locus_tag="VBC28_000436"
     CDS             complement(41512..41892)
                     /locus_tag="VBC28_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000452064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HEO6814438.1"
                     /translation="MEVIREQEFVNQYHYDARNLEWEEENGTPKTNFEVTFQLANRDE
                     AAKVTSIVAVLQFVIVRDEFVISGVISQMAHIQGRLINEPSEFSQDEVENLAAPLLEI
                     VKRLTYEVTEIALDRPGVTLEFNS"
     gene            complement(41964..42497)
                     /locus_tag="VBC28_000437"
     CDS             complement(41964..42497)
                     /locus_tag="VBC28_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6814439.1"
                     /translation="MTMKTIEEIYKNYPNIPYISPERDLAEINFSKVVPRKNMEETSE
                     GLLPGDIILLWRIQFGTFTTETSFSKYFEYIYGINGKEHLEFLIKNGFVRMESPLDSL
                     DHLSAPLLKLFLKEKNVKGLSKMKRSDLDQAIAIAFTEEELGKLFVVRGLALTEKGLA
                     ALSNNQEVIDRHPKKKF"
BASE COUNT        13818 a         8455 c         6848 g        13408 t
ORIGIN      
        1 aataataaga atcatggcag aaatttattc acaaaacttc aattagaaaa tcgtttaatt
       61 attcaagaaa tactttataa tgatattcta aatccatatt aaacaaaaca cccccacaaa
      121 atattgtgag ggtgtttgtg gggaagattc aattgaatct ttcctttttt aatcgcctta
      181 actttaacga aaagacttta aaactaggct ttcaccttta aacaaccaat agatgattat
      241 ttaagagtaa ctgaagcacc tgcagcttca agtttttctt tgatttcgtt agcttctgct
      301 tctgaagcac cttctttgat aactgatggt gcgttatcaa cgatagcttt agcttcttta
      361 agaccttcgc ctgtgatttc acgaacaact ttgataacgc caactttttt gtctccagca
      421 gctgttaatt caacgtcaaa tgagtcttta gcagcagcag cttcaccacc agcagcggca
      481 gcagctacag gagcagcagc agttacacca aattcttctt cgatagcttt tacaagatcg
      541 ttaagttcaa gaattgtagc ttctttaatt tcagcaataa tattttcaat gtttaatgcc
      601 attgttattt cctccaaaaa tatgttttaa tataaaatta tgtagctagg ctacttgaag
      661 agtgcttatg ccacttcttc gtctttttct gcaactgctt tgacagcata tgcaacgttg
      721 cgtactggcg cttgaagtac tgaaaggagc attgaaagca taccttcttt gtttggtaat
      781 ttagcaagag cgttaatttc ttcaactgaa gtgaatttac cgtctactga accacctttg
      841 atttcaagag cttctgcatc tttagcgaaa tcgctaataa ctttagctgg tgcaataaca
      901 tcttcgttag aaaacgcaac tgctgatgga ccaacaaaaa gttctttaag gtcttcaaga
      961 cctgcttttt cagctgcacg agttaaaatt gagtttttga taactttaaa ctcaacatcg
     1021 ctttcacgaa gtgaacgacg taagttagta tcttgctcaa cagtaagacc acgtgaatca
     1081 acaacaacga tacttgctgc tgctttcatt ttctcagcaa taagttcaac ttgttcagct
     1141 ttttttgcaa taattgcttc actcattagt tttacctccg tttttatttt ttgggatagg
     1201 cacaaaaaaa tcgcacctaa acctagacac gaaagtacaa tacgttattt tataatgacg
     1261 ttttgtgcct cggtaggaat attatgagca ctgctcccct actgtcttag gtcagtttca
     1321 tcagaaactt taaatagtat aacttataat tctagcattg tcaataattt ttattatttt
     1381 tctgaaataa ttttatagta ccaaaaaata ctaaaatagt tatctttata tcaaaaaaat
     1441 aatattttag tatttattta ttgaccttta ctgactaaag gtgtattata gtaccagaaa
     1501 gataattaaa tatttaaatt agacctctct atatgtatta aagtatagga atcaataatt
     1561 aactttcaaa aattaaaatc aattgaggta tttaatatat ggcaaataat aatttttata
     1621 atcgtgaccc ttttggaaat atggatgata tttttaattc tctaatggga aatatggggg
     1681 gctataacag tgagaataag cgatacttaa tcaatggacg ggaagttact cctgaagaat
     1741 tcagtcaata tcgtcaaact ggaaaactcc ccggtcaaga gctaaataac caaaacactc
     1801 ctacaaacca agtctctgca gatagtgttt tgactaaatt aggaactaat ttaaccgatc
     1861 aagcacgtca gcatttgcta gatccagtta ttggacgaaa caaagaaatt caggaaactg
     1921 cagaaattct agctcgtcgt actaaaaata atcctgttct ggttggtgat gcaggtgttg
     1981 gtaaaacagc tgttattgaa ggtttggcac aagcaattat aaatggtgat gtccctgctg
     2041 ctatcaaaaa taaagaaatc atttcgatag atatttctag ccttgaagct ggcacccaat
     2101 ataggggtag ttttgaagaa aatattcaaa atatcataaa agaagttaag gaaacaggaa
     2161 atattattct cttctttgat gaaatacacc aaatattagg agccggttca actggtggtg
     2221 atagtggctc taaggggctc gcagatatac ttaagcctgc actttcacgt ggtgagttaa
     2281 cggttatagg tgcaactact caagatgaat atcgtaacac cattctcaag aatgctgcct
     2341 tagcacgtcg ctttaacgaa gttaaagtca atgcaccttc tgcacaagat actttcaata
     2401 tccttatggg gataagaaac ctctatgagc aacatcataa tgttgtttta cctgactctg
     2461 ttttgaaagc agctgtagat ttatctattc aatacatccc acaaagaagt ctccctgata
     2521 aagcaattga ccttattgat atgactgctg cacacctagc tgcgcaacat cccgtaactg
     2581 accttaaatc cttagaaaaa gaaatcgctg agcaaagaga caagcaagaa aaagctgtca
     2641 atactgaaga ttttgaagaa gctcttaagg tcaagacacg cattgaagag ttacaaaacc
     2701 agattgataa tcataccgaa ggacaaaaag tcactgcaac tatcaacgat atcgctatgt
     2761 caattgagcg tctaactgga gttcctgtat caaatatggg tgctagtgat attgaacgct
     2821 taaaagaact tggcaatcgt ctaaaaggta aagtcattgg tcaaaatgat gctgttgagg
     2881 cagttgcgag agctattcgc cgaaatcgtg ctggtttcga tgatggtaat cgtccgattg
     2941 gtagctttct atttgttgga ccaactggag ttggtaaaac cgagctagca aagcaattag
     3001 cttttgatat gttcggatca aaggatgcta tcgttagact tgatatgtct gaatataatg
     3061 atcgtacagc agtttctaag ctaattggag cgactgctgg ttacgttggt tatgacgaca
     3121 atagtaatac actaacagaa cgtatccgtc gaaatcctta ttctattgtt cttcttgatg
     3181 aaatcgaaaa agctgacccg caagttatca cgcttctcct tcaagttcta gatgatggcc
     3241 gtttaactga tggtcaagga aacactatta actttaaaaa tactgttatc attgcaacct
     3301 caaatgcagg ttttggaaac gaagcattta caggtgacag cgataaagac ttgaaaatta
     3361 tggaacgaat ttctccatat ttccgtccag aatttctaaa tcgtttcaat ggtgttattg
     3421 aattctctca cctaagcaaa gatgacttaa gcgaaattgt agatttgatg cttgatgaag
     3481 ttaaccaaac aattagcaaa aaaggaattg accttgtggt agatgaaaat gttaaatcac
     3541 acttaattga actgggttat gacgaagcaa tgggagtacg tccattgcgc cgtgtcatcg
     3601 agcaagaaat tcgagatcgc atcacagact actatctcga tcatacagac gttaaacacc
     3661 taaaagctaa tttgcaagat ggccaaatcg tcatttctga aagataaaaa caaaacccgt
     3721 ttggaagaat ccaagcgggt tttctagtgg attgaaaaag gaaatcaaac ttaccctaac
     3781 cttcttcaag atactttact tattttaaat gtgcagagag gtcagcaata tcagctggtc
     3841 tagttcggca acaacctccg acaacttggg cgccaagttt ttgccagtca cttgtatttt
     3901 caagtagggt gtgtgaatga tcagggctag attgccaaga ttggcttgcc ccatcataaa
     3961 cctctccaga gtttggatag gtcataagag gcttgttagt ctgctctgct attgcttgca
     4021 agaaatcagc aactaacgat ggacttgaac agttaatgcc cagcgccacg acctgtgggc
     4081 taacatcaat agctttcgcc aagtctgcaa cagcactacc atctgagatg gtcttaccat
     4141 cttgagaggt gaatgacata taagcctcaa cctgagggaa atcctcggca agtagctcaa
     4201 taagagcctc agcctcctga gcgtttggaa tggtttcaag tgccagaatg tcaacaccct
     4261 catctaaaag aagctcaata cgatggcgat ggaagttttt aagaccttgc ttatcaatat
     4321 cgtagagtcc agtgtactca gagccatccg ctaaaaaggc agcgtaggga ccaacatccc
     4381 cagaaatcaa aggatagatt ctctctgatt tttcttcttt tgtcaaactt ttccaaactt
     4441 gctcacgcgc agcttttgcc aactgaacag tcaagcgaat caaatcttct gcctgacttt
     4501 cagagacacc aacttgagct agtccttgta aagtcgcctg ataagttgat gtcgtcacaa
     4561 tatcagcgcc agcacgaata taatcttcat gaatggtctg aatagctgct ggatcctcaa
     4621 tcagatattt ggctgaccaa agtttcccag aaacatcaca acctctactc tcaagctcgg
     4681 tccccaaggc accatgtagg atgagagttt ttttactctc taaaagttct ttaaatcttc
     4741 ccataagatc accatttctt gtaacgtagt ttgtgataaa tatagcagaa aataataaat
     4801 ggaacaccga agtaaagccc agcacgttgt gagctatccc aagcaatccc tacaatcgaa
     4861 atcaagagca aaatgattgt gatataaggg agaactggtg taaaaggtgt tttgtaagac
     4921 aaatcttcta gttgattctc tttcaagaat tccttgcgga aattgatttg tgcgactgga
     4981 atggctagcc atacgacaac aacagcaaag cccgcgattg aaaccaaggc tagataaact
     5041 gtgtctgcag cgtaaatact tgaaaagagc gacagcactg ctcctgccat tgacaagaga
     5101 agagcacgca ttgggacacc gtgtttattg attttcacaa cagattttga caacatacct
     5161 tcattggcaa gggaccagag catacggctt gatgcgtaga gacctgagtt accagcagac
     5221 aggatggctg tcaagataac gaagttcatg atatccgccg taaaagggat tcccatcttg
     5281 tcaaagacat caacgaatgg tgctgtggat acgcctgcct ctttcattgg caatagcgaa
     5341 gctaggacaa caattgtcag tacaaagaaa acaaccaagc gaccgattgt cgttttaata
     5401 gcccttggta cagcttcttt gggattatcc gtttcaccag ccgcaatacc aattaactca
     5461 gtaccagaga aggcatagtt aacagccaac atgactgaga caactgcaac gataccattt
     5521 ggaaaggcac cattggcagt cagatgagtg aagagaatag ccttgtgctg accttcaaag
     5581 gaaactagac caaacatagc acctaagccc aagataataa agataatgat agcaataacc
     5641 ttaatacttg agaagaaaga ctctgcttct gcaaaaaagc gtacgctaag agcatttaaa
     5701 ccaaaaatca caagggcaaa aaaggaagca aatgcccaag ccggcacatt tgggaaccag
     5761 cgctgcatca gcatggcagc acctaaaaat tcagtcccca aggcgaccgt ccaacaaatc
     5821 caatatagcc aagcaacagt aaaacctgtt ccaggactga taaacttagt ggcataagtg
     5881 tggaatgacc ccgtcaccgg catggcaacc gccaattccc caagtgatag catgaccaaa
     5941 taaaccacaa cggcaccaat caaataagac agcacagctc aaagcggacc agcctgtgca
     6001 atggtataac ctgaactcaa gaaaagccca gtcccgataa caccacctag cgataacata
     6061 aagagatgac gactggtcat cttacgctgg aatttacctt catcttcata acgatgattt
     6121 tccataacaa cctccaagaa actaatactt ttatcctacc acaatctagt cgtattgggg
     6181 aataggtatt agctatcata tatgataaag aaaaactatt acaaaaaatc agcgaatcat
     6241 aaatagcggt aaccttattt ttttatcctt acaaaattaa cgaccttatg aaaacggtcc
     6301 atttgccaag gactaataat ataattggtc tccttatcct tcaatagctg tacaggtgca
     6361 ataatgctag ttagtacaca gacaatcaac atgataaaac cacctagcca atctccccaa
     6421 gtgagcattc cttctgcact agcttgatag atgtatgcgc aagctattgg tactaggaga
     6481 aatactgtcg ttgctagacc aggattatac caagttttaa gtttaatatt catctgaaaa
     6541 ccatgaccca agagctgcgt tagactaaag aacatgactc ctaatccaag ccaataagct
     6601 tgaggaaaag caatactagc aatgtaaagc aaccaagcaa tagtattaac caacataata
     6661 gactgagtat tgcctggaaa acaatccatc agctcttctt catcataaac aacatagtta
     6721 atgataggtg atgccccacc gggaaactga tattcttcaa attgatgcgc tagaagtgcc
     6781 ataaagttaa aatacaatag atagtgaagc attgaccagt caggtctaaa aacaagcaat
     6841 attactgcta aaataataaa taaaattgct ccaaaacgat accagtaatt tctaaaatat
     6901 gacatgataa tacctcctat ttaaactgtg tttcacgaaa atgtgttaaa aatggttttg
     6961 agcgtgaaag atcaacatca ctaaatgcgt aatttgaatg tcgacctaaa cgcctataga
     7021 ttggggaacg aaaggctata aagtagttaa gaacaatcca tatcagccta aaaaccaatc
     7081 aagtccagaa tacaacttcc aatcatgagc taaataatag acagaaacag ggacaagacc
     7141 tactaaggtc gtaagaagtc ctggattata ccacttcttc aaggaaagat taaaaaagaa
     7201 aagatgcatg gcaagttctg caaatgcaaa aatcatgact gcaagtgtta aaaatggtat
     7261 attgggacaa aaaatcggca aaagataaac aatcactgca aaccactgat tcccgaaaaa
     7321 ggctgataga tggtttaagt cccacttacc agtatcagtt tcaaccgatt taagttcaac
     7381 tagcatagtg ataagcggaa aacctccagg gaaaccaaat tcttcgaaaa agtgtaatcg
     7441 aataaaaagt cccgaaataa gtagtgactg cactcgtaaa tcccaatttc caaaaaataa
     7501 tgctaaggca aaaagagaag caagataggg acaaaagagg taccattttg ttatcaatgc
     7561 tttcataaga tgacctccaa gatttgtgac atttgatacc atatagtata atcaagtgaa
     7621 cctataatat caataccttt ggaggtttta tgcacaaatg actgaaagtg tgacagttaa
     7681 taaaaagaga ataaaacaaa tcactcaaac acaagaggat atcttaacag cttttggcca
     7741 acttttggaa gagtatccct atgagaaaat tcagattagc caaattgcca agaaatctgg
     7801 ctatgctaga aggacctttt accgacactt tgacagtcga gatgacctac ttaccctttt
     7861 catcgaaaga ctaacattaa atctctttaa gcaacttggt caattggagc aacctacttt
     7921 tagccaagtt ttccaaaatt tttttagtta ttggtctgat tataaatccc ttcttctcat
     7981 tcttagaaag aatgacctac tccctcaatt tcaacaatcc tggttccgac atattgattt
     8041 gatagagtta ggtcgtggtg atttatcttc caatacctat gctcagcgat ttgcgattgg
     8101 tggtattttc agtgttctaa ttgagtggat tcatcaggac tgccaaacaa gtatcgaaga
     8161 attaactcaa gtttccttcg atattatcaa tcatttaaaa aattaaaaag agctttgcag
     8221 tagtctcaac agctactgca aagctctttt tgtcataaac tctcaaatta taactcagat
     8281 aaaatagtat cccatgattc atcaagacct gtgcggtcaa cagatgaaaa gacgatgaaa
     8341 tggtcctttt tatcaaagtt taatttcttc ttaatagacg actcatgttt attccacttg
     8401 ccacgaggta ttttatctgc ctttgtcgct acaataatta ctggaatctc atagtactta
     8461 agaaattcat acatttgaat atcgtccgca cttggatcat gacgaaaatc aactaaacta
     8521 acaacaacac gaaggttatc acgtgttaca aggtactctt caatcatctt gccccactta
     8581 gcacgctcag ttttagacac cttagcgtag ccatatccag ggacatcgac aaaacgcagt
     8641 ttgtcatcaa tgttataaaa gttgagcagt tgtgtcttac ctggcttact cgacgtacgc
     8701 gctaggtttt tacgaccaag aagcgtgttg ataaagcttg atttaccaac atttgaacga
     8761 ccagcgagag caacttctgg gagatcatcc tgaggataat gtgacttgtt agctgcactc
     8821 aataaaatag acgcattatg agtatttaaa aattcttctg ccatctctat ctccctaagc
     8881 cgtctctaaa acaggcttgt cagtaccctc aacagcagcc tttgtgatac gaacttttgt
     8941 tacatcttct tggcttggaa tttcaaacat gatatcaagc atcgtttctt caataataga
     9001 acgtaaacca cgtgcaccag tcttgcgctc gatagcctta tcagcgatag ccaatagagc
     9061 atcctggtca aattccaatt ctacaccatc ataagataat aaggtttggt attgtttaac
     9121 caaagcattg cgtggttctg tcagaatacg aaccagatct tctgcagtaa gaagttctaa
     9181 cgctgcaact actggtaaac ggccaataaa ctctggaatc agtccaaact tttgaatatc
     9241 ctcagaaatt atctcttgca tataagaagc gttgtcatca attttacggc ttgtctgtcc
     9301 aaaaccaata actttttcgc ctaaacgttg cttcacaagg tcttcaatac catcaaaagc
     9361 accaccgaca ataaaaagga tgttcttggt attaatttga atcatttctt ggttaggatg
     9421 tttacgccca ccctgtgggg gaacacttgc taccgtaccc tcaataattt taagaagggc
     9481 ttgctgtaca ccttcaccag acacatcacg tgtgatagaa acattttcgc ctttcttagc
     9541 aattttatct atttcatcaa cgtagataat accacgctca gcacgttcga cattataatc
     9601 agcagcttga atcaatttaa gaagaatatt ctcaacatct tctccaacgt atcctgcttc
     9661 ggtcaatgaa gtcgcatctg caatagcaaa cggtacatta aggcttttag ccagtgtttg
     9721 tgctaagaag gtttttcctg agccagttgg accaatcatc aaaatgttgg atttttgcaa
     9781 atctacatca tcgtcactac tctcggtata actaacacgc ttgtaatgat tgtagacagc
     9841 aactgctaaa gcacgtttag cacgatcttg ccctacaaca tattgattta atatttctaa
     9901 tagttcctta ggttttggta cttctgctaa atcagccagt acttcctcag ctaattcttc
     9961 cttaataatt tcttgtgata aggccacaca ttcattacaa atgaaaacac cattacctgc
    10021 aataattttt tttacttcat cttggctttt gccacaaaat gaacaataga cattcatatc
    10081 gttattacga ttcccagcca ttttttcctc aacttttcta ttcatttaat ttaaaataat
    10141 gttccataaa aggcatggat tgtgttgaca aggttagtga aagcattcat caaaaaaact
    10201 gttcctaatc catatctttt tttctttata gcaggaattg cacataaaac acaaatcaca
    10261 gctaaaaatg cggtaaagac cccaaaaagg ctacgttgca ttgaatagta ttccttcctt
    10321 atttttcaaa atatgtcaca gtaaaagcat gagcattctg ctcatccttt tcaaaataat
    10381 ctctagagat aactttaaac tcagatagat taacatcagg aaaataggta tcacctttaa
    10441 attttccatg aactttagtt tttatgattg cttcacaata aggcagaaat gctttataaa
    10501 tacttgcacc acctacaata aataaggtct tattatgttc ctgaaaccat tttataactt
    10561 gttcaacact atttaggact gtcactccat ctgcttggaa ttgttcatct tttgttaaga
    10621 tgattgtctc tctaccaggt aaaacacgac ggttcattcc atcaaaggtc tttcgtccca
    10681 taagcaaagc ctgccccatg gtcgtttctt tgaagtgatg taactcttta ggaagcctcc
    10741 atggtaaacc gccattaact ccaatcaaat ggtcttcatc ttcagcccaa atggcaatta
    10801 tttgctttgt catgactttt ttcctcacta acttatctct agatattcta acaaatttta
    10861 cctcaaattg cttgttaaaa aacatgagat agttagattg ctaaatcaaa acgtaattga
    10921 ggtttaattg ggtcataatc aactaacgca aaatcatctg gtttaatatc aaaaaagtct
    10981 gttccatctg gaacattcaa tactagtttg gggttacact cagacggttg gcgttttaag
    11041 agttcctgag cttgttcaaa ctggttatca taaatatgaa gattattgat aaaatagaaa
    11101 aacttcccaa ctctccaacc aaaatgcttt gcaatcatca tctgaagtgc tacatactgc
    11161 atagcattaa tatgatgtgc aactaacatg tcattagaac gttgtgttaa tgtggcatcc
    11221 aaataaagct caccattaac gcgtctcaca tcaaacatcg tttgaaaagc acaaggtagt
    11281 aggccttctg tttcttcaaa tgcttcatag tcccataatg aaataacatt acgacgattc
    11341 caagggttat cttcaagctg ttttaagagt tttgatataa tattatgttt tttaacaact
    11401 gcaccgtagc gctgacctat cgtgccagta tggccaactt cccaatcatt ccagtatgtc
    11461 acaccatatt tatcattcaa aactgctaaa tcatttgtct gatcttgata aatccagaaa
    11521 atttctttaa tagcagattt tattggaatt ggacgtagag ttgtaatagg aaattcaccc
    11581 tttgataaat catactctgc aaaggcacct gtaatatact tagaattagc catttcccca
    11641 tttttatatc taggtctggc ttgctcgcta aatacacctt cagacataat tttcgtgata
    11701 ttgtctttaa ataataaatc ggcttttgtc atgagatacc tccaaagtct tattttatca
    11761 gattttaaaa agatattcta gtgagaaaca agactttgaa ggttaaagct ttttttcata
    11821 tttttagctt ctttatggta aaatggaatt tagcaaaaat gacttttatt cagaaaggtt
    11881 taccaatgaa aataggaatt gataagattg gttttgcaac cagtcaatat gtccttgaaa
    11941 tgactgattt ggctattgct cgccaagttg accctgaaaa attcagcaag ggattattgt
    12001 tagattcact tagtattacg ccagttacag aagatatcgt tacacttgca gcttctgcag
    12061 ctaatgatat tttatctgat gaggacaaag aaactattga tatggtgatt gtcgctacag
    12121 aatcaagtat cgatcagagt aaggcggctt ctgtctatgt acatcaactt ttagagattc
    12181 aacctttcgc aaggtcattc gagatgaaag aagcatgcta tagcgcaact gctgctcttg
    12241 attacgctaa attacatgta gaaaaacatc cagattctaa agtattagtg atagcttcag
    12301 atattgctaa atatggcatt aaatctactg gagaatccac tcaaggtgca ggcagtatcg
    12361 ctatgcttat tagtcaaaat ccatccattc tagagctaaa agaggaccat ctagcgcaaa
    12421 cccgtgatat tatggacttt tggcgaccaa attattctga tgttccttat gttaatggca
    12481 tgttttcaac aaaacaatat ctagatatgc taaaaactac ttggaaagaa tatcaaaaac
    12541 gttttaatac tagtctaagt gactatgctg ctttttgttt ccatattcct tttccaaaat
    12601 tagctttaaa gggctttaat aaaattctag ataataactt agatgaacaa aagaaagctg
    12661 aactacaaga aaattttgag cactctatta cctatagtaa gaaaattgga aattgctata
    12721 ctggctcatt gtatctagga cttctatcgt tactagaaaa tagtcaaaat ttaaaagcag
    12781 gtgatcaaat tgccttcttt tcatacggta gcggagctgt tgctgaaatt tttaccggtc
    12841 agttagtaga cggctaccaa aataaacttc agagcgatcg tatggatcaa ctcaataaac
    12901 gtcaaaaaat tacagtaact gagtatgaga aactcttctt tgaaaaaaca atcctagacg
    12961 aaaatggaaa tgctaacttc aacacctacc ggactggcac tttttctcta gactctattt
    13021 gcgaacatca acgtatctac aagaaaatta ataattaata tgacaaaaat ttcctggaca
    13081 ggattttcaa aaaaatcccc tgaagaacgt attcattacc ttgaagaaca agatttttta
    13141 gctgattctt ccctagaaat agtgacgaat caggacttac tctctttatc actagccaat
    13201 caaatggctg aaaatgtcat cggtcgaata gccttaccat tttcacttgt cccagatgtt
    13261 ttggttaacg gtaaagtcta tcaagtccca tatgtcactg aagaaccgtc agttgttgct
    13321 gctgcaagtt ttgctgctaa aattataaaa cgatctggtg gttttctgac aactgtccat
    13381 aatcgtaaaa tgattggaca agtagccatt tatgatgtac aagatagtca acacactaaa
    13441 gaatctatat taaatcaaaa acagcaatta ttagaaatag ccaacgctgc tcatccatca
    13501 atagtcaagc gtggaggcgg tgcttgcgac ctaactattg agattaaaga agactttttg
    13561 attgtttacc ttatggttga tacaaaagaa gccatgggtg ctaatatggt taacaccatg
    13621 atggaagcat tatcttcgcc attagaagac atttcaaaag gcaaaagctt aatgtcaatt
    13681 ttatctaact atgctaccga atctttagtg actgctacat gccgagttga tttaagattt
    13741 ctaagtcgcc aaaaagagga ggccatcaag ttagctcaaa aaatgacgat ggcaagtcaa
    13801 ttagctcaag tagaccctta tcgtgctagc acacataata aaggtatttt taatggcatt
    13861 gatgctatcg tcttagctac tggaaatgat tggcgagcta ttgaagctgg agcgcatacc
    13921 tatgctgtca aagatgggca atatcgagga ttatctagat ggtcttataa ggttgatgat
    13981 aattgcctag aaggtactct gacgctaccg atgcctgttg caacaaaagg tggatccatt
    14041 ggtattaacc ctagtgtcca cctagctcac gatctactag gtcgtcctaa tgctaaagag
    14101 ttggcaagta ttattctttc gattggacta gctcaaaatt ttgcggctct taaggcattg
    14161 gttagcacag gaattcaagc tggtcacatg aaacttcaag caaagtcgtt ggcactactt
    14221 gctggagcta aagaagaaca aatttcagaa gtcgttaaac agcttctaga ctctaaacac
    14281 atgaatctag agacagcaca aaaaattgtc aataaactta ctaaaagtaa aaaaagaatt
    14341 tgagaaaaat catctcaaat tcttttttta cttgacttga atttccataa gtgtataatc
    14401 tagttataaa gactagatta tatggagttt taaaatgaat aaaaatcaat acccaaaatg
    14461 ggaagaatta cctgaactag atttgtatct tgaccaagta cttctctatg tgaatcaatt
    14521 aattaatcct aaaacaataa ctaacgataa actactcacc gcttcgatga tcaacaatta
    14581 tgtcaaacat aattatatat ccaaacctat aaaaaaaaaa tacaatcgcc gtcaagtagc
    14641 acgattgatt gttattacag cttttaaaca agtttttgct attcaagaaa tcagtcaaac
    14701 attagaactt ctgacagctg acaatcattc tgaagaagca tataacggat ttgctgcttg
    14761 tatgaacaaa gaagaggttc acgatttacc accagttgtc attagtgcct gtcagacgtt
    14821 gaacctttat caagaaacac aaaaattagt tttagaacta ggaggaaaaa ataaaaatgc
    14881 ttaaagaaat gtctatcaaa ttaagtcctc aactttcctt tggcgaagag cttgcaaata
    14941 gtatcactca cgctgtagga gctttgctta tgctaatcct attaccaatc accgctgtct
    15001 atagtcacaa ccacttcgga ttacaagcag cacttggtac ctcaatattc gtcacttctt
    15061 tatttttgat gtttttatca tcaagtattt accactccat gacttataac agtcttcaaa
    15121 aatatgttct acgcatgatt gatcactcta tgatttatat tgctattgca ggtagttata
    15181 ctcctgtcgc attgtcactt attggcggct ggttaggata tctcatcatt tttttacaat
    15241 gggggattac attatttggt atcctttata aaatcttcgc tcccaaaatc aatgataaat
    15301 ttagccttgt actttacctc attatgggat ggctggttat ctttattttc cctgctatta
    15361 tcacaaaaac tggaccagct ttttggggat tactcttggc tggtggtatt tgctatacta
    15421 tcggtgcact cttttatgct cgcaaacgtc cttatgatca catgatttgg caccttttta
    15481 ttctattagc ttctatttta caatacattg gaattgtcta cttcatgcta taaaatttaa
    15541 ctacatttta ttgattattt ttttgctaaa ccatgatata ataataatca tctaaatctt
    15601 agagagaagg cgtggacata tgaaaagtgc ttatatcttt tttaatccaa aatctggtaa
    15661 agatgaacaa gcattggcac aagaagtaaa atcttattta atagaacatg attttcaaga
    15721 cgattatgtt cgtattatta caccttcatc tgttgaggaa gctgtcgcct tagcaaaaaa
    15781 agcatctgaa gaccatatcg acttggttat tcctttaggg ggagatggca ctattaataa
    15841 aatctgtggt ggtgtctacg ctggtggtgc ttatcctact atcggactag tacctgccgg
    15901 gaccgtcaat aacttttcaa aagctttgaa catcccgcaa gagaggaatc tcgctcttga
    15961 aaaccttcta aacggtcatg taaagtccgt agatatctgt aaagttaatg acgattacat
    16021 gattagcagt ttaacacttg gtctccttgc tgatattgct gctaatgtca cttctgaaat
    16081 gaagcgtaaa ttaggccctt ttgctttctt aggtgatgcc taccgtatat taaagcgtaa
    16141 tcgatcatat tctatcactc ttgcttacga taataatgta cggtctttac gcacgcggtt
    16201 actccttatt accatgacca attcgattgc cggtatgcca gccttctctc cagaagcaac
    16261 aattgatgat ggacttttta gagtctacac tatggaacat attcacttct tcaaattatt
    16321 gttgcatcta agacaatttc gtaagggaga ttttagtcaa gcaaaggaga taaaacattt
    16381 tcataccaat aatctgacta tttcaacttt taaacgtaaa aaatcagcta tccctaaagt
    16441 tcgtattgat ggcgatccag gtgaccaatt acctgtaaaa gttgaagtca tccctaaagc
    16501 cttaaagttt atcattccta actcattacc ataatataaa aggacctaga cataagtatt
    16561 agagtaaata ctatctgtct aggttctttt ttgttatact taaagcaata aatatcattg
    16621 aaaggaagta tacgatgaca acatttactg ctaaatttat tgaccaagag tgggaagtcc
    16681 cagtagaatc aggtcgctac catatgattg ttggggaatt ttgtccttat gcacaacgtc
    16741 cgcagattgc gcgccaactg cttggtttgg ataaacatat ttctatcagt tttgtggatg
    16801 atgttccaag tgatatagga cttattttta gtcaaccaga acaggttact ggtgctaagt
    16861 cgcttcgtga tatttatcat ttgactgacc cgacctacca aggtccttat actatcccaa
    16921 ttttaattga caagaccgat aatcgtattg tctgtaagga atctgcagat ttgcttcgac
    16981 tttttactac agatttctct gatttgcatc aagaggatgc tcctgtcctt ttcagccaag
    17041 aaactgcttc acttattgat aatgacatta aagacattaa taagaacttc caaagcttaa
    17101 tgtataagtt agcttttctt gataaacaag cagattatga tacttatagt aaggaattct
    17161 tcacttttct agatcaaaaa gaacacttac taggacaacg tcctttcttg ctcggagata
    17221 atctaagtga ggttgatatt catttcttta caccattagt ccgatgggat attgctgggc
    17281 gtgatcttct actacttaat cagaaagcgc tagaagacta cccaaatatt ttttcttggg
    17341 ctaaaacttt atacaatgac tttaatttaa aaacattaac caatccacaa tccataaaaa
    17401 ataactatta cttaggcaaa tttggaagag ctgttcgtca tcataccatc gtcccaacag
    17461 gtccaaatat ggcaaaatgg gaaaaataaa gctaaaaagc taaggcaatt cacagcctta
    17521 gctttttagc tatactagtc caaagtggac caatcaaatt caataaaagt cttgaagaga
    17581 tctccataag taccactgta agcaatatct ttatctttat aagtcatttt cgtaatagga
    17641 taattatcag gaacagtaac acatcccatt aaaactcgaa taaaatcatt tggattttca
    17701 aaaacttggc atcgttcagt gtcgtaagca tctttataat acagtttaat catcttatta
    17761 cctctgacgt tttacaagga aactttgaca gtaagctata tctacttcac aaaaataata
    17821 tcatttagca ttggcttcct tcaattgacc atacaatata taattgactt gtcgtaaatc
    17881 agttattttt ttacaattca gtgcacacat tatcattctt aaatcttctt tccaagaatt
    17941 gagaattgca ataacatcat ctacaggata acgctcaact aattctaaaa ctgttctaga
    18001 aagtccaaca gctttagcac ccaacactaa acattttacc atatctaaag gatgacgaat
    18061 gccaccagat gctaggatat ccattttgtc catcatagat tgagcgttta ttaaggattg
    18121 agctgttgtt tgcccccagg tattgagata cgaacggtcg cgtccacgtt gattttcgat
    18181 gtaagcaaaa ctagtccccc cacgccccga aatatctaca gttgtgatac ctatatcgta
    18241 agcatccttt attgattgta aatccatccc aaaaccaacc tcttttaaaa taagtgggca
    18301 ttggatattg tcgacgtatt cttttaagtg agaacgccac atgtgaaatt cacgctctcc
    18361 ttcaggcatc aaaagttctt gcatcacatt aacatgaact tgtaaaaaaa tggggttcat
    18421 agccttaacg gttgactctg ctgctggtac aggcttgtct aaccctatat ttgtcgccag
    18481 ttttaaatca ggatacaagt ctgttgttgg gtaggaatca tcttcatcgt ttttgagagc
    18541 tgccgaatat gaacctgtca ccataacaat ccctgtagct tgagcaacct gtgccaattt
    18601 atgattgact gctttccctt tttcactacc gcctgtcata gcattgatat aaaaaggaaa
    18661 ttcaaagctt tgaccagcaa aatgtgttga taaatcgata tcgttgacat tatattttgg
    18721 taaggatgaa tgtattaact caatatcgtc aaaactatta taaggcgact gatatttaag
    18781 agcatatttt atgtgatcat cttttcgatt tgtcatgaca gtgtctcctt actaagtagt
    18841 tcaataccag cactctccca acgcttgatt agagtttgat tatcattttt attaaaggat
    18901 atggctatac cacaatcgcc tcctccagat cctgaagatt ttgtgactat atctaggcct
    18961 ttgcttgcct ctttaagctt ttgaagttta tcggtgtaaa tagatggatg aagattaaat
    19021 aatagcaagc taatttcttc taaacatctc ttaatcgctt ctttatctcc ttctttaaag
    19081 gctgaaagta actggacaac ttgatacttt gtatcatcta aaaaacgctg cgtaatagtt
    19141 gatttaacta tatttatcat ttcttttgaa atggacggtt gcattgtcca tcccactaaa
    19201 aaatccatct ccaatgaagc ttgtattttg gtaatacgat aaccccattc agcttccaat
    19261 acttgttcca aaggttttgt ttcaatcatt tttgaaacag ccctacgatc aaaagctgaa
    19321 taagaaatta aatgctcata agcaatacaa gcaatatctc ccattgagcc attatcacct
    19381 ctttttaaga gaacataggc agataattta aaaagcaagt cgcttggcat ctctatctca
    19441 taaagcgcag ccatagcttt aatagtcaaa acgacaacac ttccgctaga gcctatacca
    19501 aatttaagtc catagcgctc taattttccc gtaatttcga ggtgaatcgg ttttatattt
    19561 ttgccaaaat taatcaacca ttcttccata agcaatatcg tttcctgaat gagactatat
    19621 tgcttatctg gctgtaagct tgccgtatag ttaaacatat cagaataaag gctgtagtta
    19681 tctgcaaaag tagctaatgc agtcatataa ataggaacgt tctttaaaat agctacttgc
    19741 ccaggataca gtatcgcgta ttcacctgca atatataatt tgccacctgt ttgcacctta
    19801 accatctgct aattcctttg tcgttgaaac aataatacga taatctttct ccaagatagc
    19861 agctaatgcc tcaaggtctt gacgtaagca aagtacttta acatttggac ccgcatccat
    19921 cgtataataa cattcatgac ctttttcgcg aagtttcttc acaacatcca tagctttata
    19981 tgtctcttct gtcaaatatg aaaaggctgg agttgctgtt ttagttgttg agtgcattgc
    20041 aagtgcatta cgctctgtca gttgacctac cttttgaaaa tcattatttt ttaggtatac
    20101 taacatatct tgataatctt gttcagattg ccttacccat tcattaaaag tagttgaagt
    20161 ttcggtgcag attttcatac cttcacggct tgaaatcggc tttcttttgt catttaagac
    20221 cagcataatc atagctaaat ctaaattagt gtgaacttta tagatatcac cactatcttt
    20281 atcccaagca gcaacaggtc caaaaaaaga gcgtgaagag gagcctgaag caaacttggc
    20341 ttcttgagct agctgtgatt ggctaaggtt agtaccaaaa aaatcattgc aggcctttac
    20401 taaggctgat aaaccacttg atgacgatga taagcctgca gctgttggca tattattagt
    20461 tgtttcaatc tttacaaagc cggacttatt ttgtcgaaag cgatccaaga tagctgacat
    20521 tttgtcatgt tcatgatcat tttgaagaac accagagatg taaaattcat ctttcttagc
    20581 atccttccca agggcagtca aacgtgtttc agtgtacatg ttttcaagtg ttagagaaat
    20641 actactggta gctggaatca ttttttcagc atctgctttt ccccaatatt tgataatagc
    20701 aatgttagca taagacttaa cacttatact ttttccgtcc atgtgttgac agctccttct
    20761 ctttctaatt gctgacttaa gcgctcagct tctctttttt cttttacaag tgcgataata
    20821 caaccaccta atccgccgcc actcatttta gcgcctagag caccacaact aatagctacc
    20881 tctaccagat gatcagcttc taaacttgag acacccaatt gttttaaact ttgatgtgct
    20941 ttagtcatca agctaccaag ttgtttggaa tcacttttat gaatagcatc ctctgatgcc
    21001 tctgctaaat aacctaattc ttttaaaaag ggtagtactg cctcgccaga tgatttcacc
    21061 ttatccactg cttctttagt atgcccatga attcctgtat cagcaataac taaataagca
    21121 ttcaaatgca tgggtactgt tgaaaaacca atattgcgga taaatttaat agtattttca
    21181 ctaagacaag tttttgcatc taatccactc ggatttgaat gtgctatcat ttctgctcga
    21241 ttaacaaggc attctaacaa atctgcctcc aaatcttcat caaaataatc aaaaactgca
    21301 cgaatagctg caatagcaac tgcagctgat gatcccatac cacgtctttc tgggacttgt
    21361 gactcaatat gatatgcaat tttagaagaa gttttaccta aatagtccaa ggcagcaaaa
    21421 atggctgtcg acaaaggatc agtcatatct aatgctatta attgaggagc ctcttcaata
    21481 aggcaagtta cttcaatatt ttttaaagga atagcaatgg ctggataacc atatactact
    21541 gagtgctcac ccatcaagat aatcttgcta tgagcctttc ctataccaaa cttttctttc
    21601 atctttttct ctttacaatt ctattacctt actattttaa tataaatcct taaaaaaagc
    21661 gactttaacg tgataataac ataaattaaa ggaactacaa ttgcagtccc tttaatctta
    21721 aatgtaaatg acaaaggtaa tggtatcatg tttataattc acatcgatac ttcctttaaa
    21781 tagatgcact aaactagtta cgatagaaag accaatacca tatcccgatt ttttgttatt
    21841 atgtgactca tcctctcgat aaaatcgttc aaaaaacttg ctatagtcta tgtctttacc
    21901 attcttataa gtatttgaaa cttcaagctt agctcgtctc aaacgacttg aacgtgataa
    21961 cttaactgtc acagtcccca tcggatcaca atacttatta gcattatcaa ccaataatgt
    22021 aaccaattca aataatgatt tttcttcagc tttgacatga atacctggtt ggatactcat
    22081 tataaagtct ttaccatctt taatgattgg acctttaaaa tcttctgcag catcttttgt
    22141 aatatgtgaa aagtctaagt caaccatact tatatcaggc tgttcttcaa agcgggcaag
    22201 actgaccatg ccattaatta gacccgttaa acgttggatt tgatcatttg tagatttagt
    22261 ccactctgat tcaccactca tcatttccac caattcatta ttagcggaga tgatagcaag
    22321 tggagttttt agttcatgtc ctgcattggt aataaaacgc ctttgctttt catagttagc
    22381 aacaaatggt aagataactc tccctgataa aactgaaaca attaccatga agacgataaa
    22441 acttaccaag gacatccata ttgaaagttg caataatctt tgattatcaa gatattggtt
    22501 ggttgtatct aaaaatacca ctaaataatc atttttacca acctttttta gtagataaga
    22561 ataagtacta aaattatagg tgatatgacc tgaatgacta ttttgtgata cgatacgctt
    22621 ggcataattt tttatttgcc tatcgcttac ttcaaagata tttctagaat caaaactagt
    22681 cacgttacct tttgcatctg ttcgcgcact gaaataacgg aattggaaaa tagcgtccgt
    22741 tgataggtca tcacctaatt ctttactaaa ttcaagttta ttcggtatac gtccattgtt
    22801 gtctgcaagg atagttaaca cacgataaat ttcattcttc gtttgatagt gattagcaga
    22861 gttgaggaca ccgactactg aaaataagac aacaagaata gctagcgcag ctattccgat
    22921 aaaacgaagt cgtaaatttc taaacattag ccaccattta tttcacttaa tttatagcta
    22981 ctaccttctt gaccaataat ctccaagtta gcaccaattg cctgcaattt ttgtctcaaa
    23041 taagaaacat agataaagac gtatccctca tctacatctt cttggtcctt atctgctccc
    23101 cagacatgtt cgaaaagttg ttgtgtagat aattctttat catggttcaa catgaaaaat
    23161 gctaacattt tagcttcttt acccgctaga cgaatagtgt tttcacattg caactcttgt
    23221 tcaccgactg ataaggttac tcttcccaat gacaatacat taggtgtgaa atcttccaat
    23281 cttcgggaca tagagcgaag ccttgctaat aattctttta gagagaaggg ttttgttaaa
    23341 taatcatctg caccagcatc tagacctgtt actctatcat caatttctgc catggctgtt
    23401 agcataataa tgtgagattt atttcctgat tgtctaattt ctttgacagc ctcaacacct
    23461 gttttaacag gcatcatgac atccataacc atgacatcat atgcattttg attagctaaa
    23521 tcaatagcag tctgaccatc gtaggcgaca tcaacaacat aaccttgatg actaattgcc
    23581 gtactaagga cgcgtgacat ctgttcttca tcttctgcaa ttaatactct cattttattc
    23641 cttctttgtt ccactctcta tcaattgacg aatcgtttgc atcgttaaat caacagatgc
    23701 tatttcatca gcacaacgtt ttaattctct tacaattcgt tcgggatttt caatatcatg
    23761 cttgtatttc atctgatgtt ctaaactagc ccaagaatct tgcgcaattg tacgcaactg
    23821 aatctccatg taatatttac cttctgaatt acccaaacag tccggatagg gagtatcaat
    23881 ttctaaaatc acatggtaag agcgataccc atttggctta acatgctgga tatagtcttt
    23941 ttcaactaca atacgacaat catcaaatgt cttaatacgt tctacaattt gataaatgtc
    24001 gttaacaaaa ccacaaataa tacggactcc aatactatct ctaataactt ttagtgcaga
    24061 atctactgtt acaggtaact gtttacgacg acatttctct atcatactat ctgatgtctt
    24121 aatacgacca atgatatgtt cgtacaattt atagcctgtt tcaaccttag ctttatcatt
    24181 ttctaattgg atacgctgac tgaaatcttc taaaattaat ggtagatatc gaccataatc
    24241 tccataaata tttgtttcca taagaaacct tcctattctc tcttaagctc cattatatca
    24301 tagaaactta aggcaaacta aaaaggaatg agaatttttt cttcccattt ccttctaaaa
    24361 tgtttatgtt aagctgattc gaattgagaa ttatacagat cagcataaaa accactttga
    24421 gacatcaaaa catcatgatt gccttgctca atgatattcc catctttcat gaccaaaatc
    24481 aagtctgcgt ttttaatcgt tgataaacga tgtgcgataa caaatgatgt tcttccttcc
    24541 ataagtttgt ccatagcttt ttgaattaac tcttctgtac gtgtatcaac agatgaagtt
    24601 gcttcatcta agataagtaa gggagaattt ttcaaaagag ctcgagcaat ggttaataat
    24661 tgcttttgtc caactgacaa tgtcacagcg tcatcaagga cagtatcata ccctttaggt
    24721 agtgtcatta taaaatgatg tataccgact gccttagcag cttcttgaac ctcatcatca
    24781 ctgattgtcg tctggttaaa aatcaaattt tctttaatag ttccctcaaa caaccatgta
    24841 tcttgaagaa ccatcgagaa agcatcatga acttgactac gtttcatgtc ttggatattg
    24901 atgtcatcta tggaaatact acctgaatta acatcataaa aacgcatcaa taaattaaca
    24961 attgtcgttt tacctgcgcc agttggtcct acaatagcaa ctttctgacc aggctgtgcc
    25021 tccgaagtaa agtcatgaat aatagtcttg ttatcactat aaccaaactt gacatgttca
    25081 aaggacacat gccccttaaa cttaggaaaa tttaacactt tgtgtttatc ctcttgcata
    25141 tttgtttcat ttaaaaattc aaaaacacgt cccatggctg cactagcaga ctgtaattgt
    25201 gtaattcctt gagcaatctg accaataggc tgtgtaaaaa tacgaacata ggtcataaaa
    25261 gcaacaattg tacccatagt aattgttcct ttgatagcta acatggcacc aaccacacac
    25321 accatcacat aaccaaaatt accaataaac tgcatcagag gcatcataat tccagagaaa
    25381 aattgtgatt tccacatgga actgtgaagt ttatcattta atagttgaaa atctgactta
    25441 gcctgctgtc ttgcattatg actagtaaca atgttgtgtc ctgagtaaat ttcttctaca
    25501 tatccagata tattagccaa attcacctgt tgctttttaa ataaaggctg actcttcacc
    25561 ataatgattg aagaaagagc gaatcctgaa aataccgata taatcgccgt tgttgctaaa
    25621 tgccaatctg ttttgaacat cataattaat gaaccaacca ataacactgt agatgcaacc
    25681 atagtaacta aactttggtt aaaagactgt gtcatcaaat ccacatcatt tgtaacacga
    25741 gataaagtat caccttgagt atggctatca aaataactca aaggtatttt attgatttta
    25801 tcagcaatag cattgcgcat accttgagca aatttttgaa taatagtcgt cacaataaaa
    25861 cttgctagat aggatactaa tgcacctaaa acatataata ctgccaattg ccctgcaata
    25921 tgaccgattt tggctaagtc aatattacca gccaagcctt ttaccataag atccgtcatc
    25981 tctttaagac ggtctggtcc tataactgtt gtcaagcttg ataaagttgc taacacaatt
    26041 gctaacgcaa aaagaagttt gtaacctttg aggtacggag ataaattaga gaaaaaagac
    26101 gttttttgtg atttagtttt cattttccaa ttcctcctta gacaattgag aataggctat
    26161 ctcttgatat acttcattat ttgataacaa ttccttatgt gttccttgcc caacaacttt
    26221 accttgatct agcactaaaa tgagatcagc atccatgata gttgaaatgc gttgagcgac
    26281 aattaatttc gtcatagcag ctgttttttc tttcagcaca tggcgtaaaa tatgatctgt
    26341 tttataatct aacgctgaaa atgagtcatc aaaaatgaga atttcagggt gacgtgctaa
    26401 agcacgtgcg attgccaagc gttgtttttg tcctcctgaa aagttactac caccttgagc
    26461 gacttctgta tctaatcctt tttctttagc ttctataaat tctttagctt gcgctaattc
    26521 gagtgcttgc caaatttgtt catcactaag ttcctccctg ccactctctc caaaaatcat
    26581 attagaacgt actgtcccac taaagagaac agctttctgt ggaatatagc caattttatt
    26641 attcaaatca ttgtgagagt attcctttat atcaatgcca tcaagtttaa ttgacccctc
    26701 tgtggcgtca taaaaacgtg gaactaaatt gactaaagtg gattttccag aacctgtaga
    26761 cccaataaat gccactgtct gaccagtatc tgctttgaaa gatacatgtt caataaccgc
    26821 ttcagaatct ttactataat gaaatgacac atcttgaaac tcaatttgac cttttggtgc
    26881 actttggata tgactatttt ctttaaaagt tactgttggg gctaaagtta gtacctcatt
    26941 aatacgtcta gccgatacta aagcacgagg catgatgata aaaatagcta ccatcatcat
    27001 aaatcccata acaacttgca tagcataaga tgaaaaaaca accatgtttg aaaaaagatc
    27061 cactcgatca gtgatagctg atttagcagc caatacagat acacttgttc ctgtaacttt
    27121 aggcacagaa atatcattaa tcaaataaga gccaatccaa taaatagcta aagtcaatcc
    27181 agatgaaacc actgtcatca caggattcat aagcgacatc aatctataaa caaaaaggtt
    27241 caattttgtt aaatcgtcat tttcacgttt gaacttatct gtttgatact tttcagcatt
    27301 gtaggcacga ataactctaa caccagagag tgcctctcta gtagttgcat taagcgcatc
    27361 tgtaaaacct tggatttgac gttgtctcgg aaaagcaagt aagagtagaa cgctaagtaa
    27421 tatcataacg ataataaccg ctactccagc tgccccagtc cattgatcgc ttttacccca
    27481 aattttagtc aaggcccata ttgccataat tggtccacgg gtaacaacct gcattcccat
    27541 tacaataagg atctgtagct gagtcaagtc gttagttgtg cgcgttaata gagaggggat
    27601 agaaaatcgc ttaatttctg catcagaata atccatcaca tggttaaaaa tatctgaacg
    27661 taatcgacta gtaaaactag ctgctgtcct agcacctaaa aaacctacca ttactgctac
    27721 taaaaaacta ccaaatgaaa aagcaagcat ttttagtcca ggatcccaaa gagcctctaa
    27781 ctttgtacct ttagtttgca acatacgtgt gatatcagac atatattctg gagtctttag
    27841 gtccatccaa accgctagac acactaatat agtactcgcc acaatcatca accattcttt
    27901 accattgagt cgcttgaaaa tattaaacat cgcaaactcc ttttactttc atatttatcc
    27961 tattatccta aaatagttaa tcaaaatctt ccatcgccat atttttagct aatgttctaa
    28021 taaccttttt ggtagtacgt atctcttctt tagtaatacc tgctaacatg gtactgtgca
    28081 atttttctaa gaaaatttca aattgtgtag ccttttgttt acctagatga gtcaaataaa
    28141 ggtatttcac acgcttatct gtcttagatg gaacaatcgc aataaatcca tttttttcca
    28201 tccgttttac caaattagac gctacagact tagaaatgtg taaaatttct tcagcatctt
    28261 ttatagacat atccttgtca ggatgtttat agagatgcat cactaagtgt ccttgtggac
    28321 ccgctaaatg ttcaacatca tactcttttg cataacgatc taaatacttt tcaagttgat
    28381 taacaagtat tcgtgctttt tgaagaggat tctccataat aatactcctt taatagttat
    28441 cgtgagaact attttaaaac aacctttatt aattgtcaat taaaagttac cgtgagaact
    28501 atttttacat taatatttct aaaaaacacc tatcattcgt caaataaaaa atgacttcgc
    28561 ttacttttta atattaaaaa tatatgttcg ttttttatat ttttaattat aaatttatat
    28621 tgatttttaa tgtaaatgat agtaaaatac aaatgtaata cttatttaat ttacaggaga
    28681 tttaaatgaa aaagaaaatt attttgaaaa gtagtgtcct tggtttagtc gctgggactt
    28741 ctattatgtt ctcaagcgta ttcgctgacc aagtcggcgt ccaagttata ggcgtcaatg
    28801 actttcatgg tgcacttgac aatactggaa cagcaaatat gcctgacgga aaagttacta
    28861 atgctggcac tgctgctcaa ttagatgctt atatggatga tgctcaaaaa gatttcaaac
    28921 aaactaaccc taatggtgaa agcattagag ttcaagctgg tgatatggtt ggagcaagtc
    28981 cagctaactc agggcttctt caagatgaac caaccgttaa aacatttaat gcaaagaatg
    29041 ttgagtatgg cacattaggt aaccatgaat ttgatgaagg tttggcagaa tacaatcgta
    29101 tcgttactgg aaaggcccct gctccagatt ctaatataaa taatattacg aaatcatacc
    29161 cacacgaagc tgcaaaacaa gaaattgtag tggcaaacgt tattgataaa gttaacaaac
    29221 aaatccctta caattggaaa ccttacacta ttaaaaatat tcctgtaaat aacaaaagtg
    29281 tgaacgttgg ctttatcgga atcgttacca aagacatccc aaaccttgtc ttacgtaaaa
    29341 attatgaaca atatgaattt ttagatgaag ctgaaacaat cgttaaatac gccaaagaat
    29401 tacaagctaa aaatgtcaag gctattgtag tccttgctca tgtacctgca acaagcaagg
    29461 atgatattgc tgaaggtgaa gcagcagaaa tgatgaaaaa agtcaatcaa ctcttccctg
    29521 aaaatagcgt agatattgtc tttgctggac acaatcatca atatacaaat ggtcttgttg
    29581 gtaaaactcg tattgtacaa gcgctctctc aaggaaaagc ctatgctgat gtacgtggtg
    29641 tcctagatac tgatacacaa gatttcattg aaaccccttc agctaaagta attgcagttg
    29701 ctcctggtaa aaaaacaggt agtgccgata ttcaagccat tgttgaccaa gctaatacta
    29761 tcgttaaaca agtaacagaa gctaaaattg gtactgccga ggtaagtggc atgattacgc
    29821 gttctgttga tcaagataat gttagtccgg taggcagcct catcacagag gctcaactag
    29881 caattgctcg aaaaagctgg ccagatatcg attttgccat gacaaataat ggtggcattc
    29941 gtgctgactt actcatcaaa ccagatggaa caatcacctg gggagctgca caagcagttc
    30001 aaccttttgg taatatctta caagtcgtcg aaattactgg tagagatctt tataaagcac
    30061 tcaacgaaca atacgaccaa aaacaaaatt tcttccttca aatagctggt ctgcgataca
    30121 cttacacaga taataaagag ggcggggaag aaacaccatt taaagttgta aaagcttata
    30181 aatcaaatgg tgaggaaatc aatcctgatg caaaatacaa attagttatc aatgactttt
    30241 tattcggtgg tggtgatggc tttgcaagct tcagaaatgc caaacttcta ggagccatta
    30301 atcccgatac agaggtattt atggcctata tcactgattt agaaaaagct ggtaaaaaag
    30361 tgagcgttcc aaataataaa cctaaaatct atgtcactat gaagatggtt aatgaaacta
    30421 ttacacaaaa tgatggtaca tatagcatta ttaagaaact ttatttagat cgacaaggaa
    30481 atattgtagc acaagagatt gtatcagaca ctttaaacca aacaaaatca aaatctacaa
    30541 aaatcaaccc tgtaactaca attcacaaaa aacaattaca ccaatttaca gctattaacc
    30601 ctatgagaaa ttatggcaaa ccatcaaact ccactactgt aaaatcaaaa caattaccaa
    30661 aaacaaactc tgaatatgga caatcattcc ttatgtctgt ctttggtgtt ggacttatag
    30721 gaattgcttt aaatacaaag aaaaaacata tgaaataata aaaataggtt tggataaaat
    30781 atccaaacct atttttatat caatatgcct tccaaatgat ctaactcgtg ttgacaaatt
    30841 tgagctggaa aatctgtcaa cttgatttgc tgttctttcc aattgatatc acgataagaa
    30901 atcgtaatgt gatgatatcg ttgtgtactt ctgcaaccta cgagagaaag gcaagattct
    30961 tctgtctcat atggatccga ttttgcaact aatacagggt taaacattac taaatttgta
    31021 atacccacat tgatgataat gacacgcttc agactaccaa tcatattggc tgccatacct
    31081 acacaataat tttgattagc atgcaaagta tcctgtaaat cttttgctaa gctcacatct
    31141 gctctactag ccatttgtga tttttgttgt aaaaagaaag tatctctaac aattggtttt
    31201 atcatttatt ttaacctaac tctattatga taatactagt ataccatagg ttagctaagg
    31261 actatgataa gagattaaac aataccttgt gcagacatag cttgagcaac ttttaaaaag
    31321 cctgcaatgt tagcacctag aaccaggtta ccactatcgc caaattcatc agccgctttg
    31381 gaagcattca caaaaatagt tttcataata cgttgcaatt cttgatcaac ttcttcaaaa
    31441 gtccatgccc tacgactaga attttgtgcc atttcaagcg ctgaaacagc tactcctcct
    31501 gcattggcag ctttagcagg accaaaagca acccctgcct tttggaaaac tttaatagca
    31561 cccaaggtag atggcatatt tgctccttct gttactgcca agacaccatt ttcaactaat
    31621 aacttagcgt cttcttcgtc taattcgttt tgagtagcac acgggaaagc taaatctgct
    31681 ttaatagacc agatactacc ttgatctgct ggtgtgaaat tagcttttgg atgtttctcc
    31741 gtatatttaa caatacgtgc tctttcaacc tcttttatct gtttcaatgt ttcaaggtca
    31801 ataccgtctg gatcataaac ataacctgaa gaatctgaca cagctacaac agttgcaccc
    31861 aactcttgta atttttcagt agcataaata gccacgttac cagatcccga aaccagtgca
    31921 actttgccag ttaaatcttg accacacgct gccagcattt ctttagcaaa atagactgcc
    31981 ccataaccag ttgcttctgt acgagccaaa gagccaccat aagtcagtcc ttttccggtt
    32041 aaaaccccat tttgataacc atttaaacgc ttatattgac cataaagata accaatttca
    32101 cgtccaccta ctccaatatc gccagctggc acatctaaat ctggtccaat gtatttttga
    32161 agttcggtca taaaactttg tgtgaaacgc atcacttcat tatctgattt cccttttgga
    32221 tcaaagttcg accctccttt gccaccacca attggtagac ctgtcagtga attcttaaaa
    32281 atttgttcaa atcctaaaaa tttgacaata gattgtgtta ctgtagggtg aaaacgtaaa
    32341 ccacctttat aaggaccaat agctgatgaa aattgtacac gatatcctcg gttaacttga
    32401 acttggcctt tatcatctac ccatggaact ctaaaagaaa tcacgcgctc aggctcaacc
    32461 aaacgttcta aaagattttc ttcaatgtac tgagggtatt tatcaaaaac tggtactagt
    32521 gactcaaaaa cctcttcaac cgcttgtaga aattcttcct catgctcatt ttgcttcttc
    32581 actttctcta aaacacttgc tacatatgtc tttccgcttg tcatttattt tcctccgtat
    32641 tgttttaata tagttagcat tataaattgt cagaattttc ttgtcaacta tttgctatgg
    32701 tatcattatt aggagcaatt ttaaaggtaa ttatttgact tttactgact ttcgttctat
    32761 aatgagacaa atatttgatt ggagtatcta tatgaaatgg ttcccacttg ttggaggtat
    32821 aattgcctta ttagctggct tctatctctt tataaatcct gtaacggttg ttgcaactct
    32881 gggttggttc attgctttat ttatctttat ttatggcatt tcaaatttag tacgctactt
    32941 gcaagctgat tcttatcacc gtagcccttg gtatctggtt cagagtatcc ttgctattat
    33001 ttttggtatc attttactat ccagctcagc aatgtcactt tcaaatactg tcatgactat
    33061 tttagcatac tggctactgg tatctggtat tttacgactt ttgggtggtt tccaaatgca
    33121 aaaaattaat ttcccaggtg ccaaatccta taaacgttcg gcttatattg cactcatttt
    33181 aggtattatt ttactggttc aacctacctt atcagcagcc atcattggaa gatttgtagc
    33241 ctttatatta atagcaattg gagcttcggc tatttacact tttactcgta ttaattatta
    33301 aaaggacatg tttcgtgtcc tttttttatc tattgatgac gtcatttact atttgaaaat
    33361 tggctattat ataattcgta ataaaatcct ttatctgcta ggagtttctg gtgattacct
    33421 tgttcgatga tttgaccatc ctttagaact aaaatgttgt ctgcctcttg aatcgttgat
    33481 agacggtgtg caatgacaaa actagttctt ccttccatca attttttcat agctttttga
    33541 ataagcaatt ccaaccttgt atcaacagat gaagtcgctt catctaaaat taaaatttta
    33601 ggatctgcta ataaagcacg tgcaatagtc aacagttgct tttgtcctaa ggagatattg
    33661 cttgattctt gattcatcac catattgtag ccaccaggta acgttcgaat aaagtggtca
    33721 acattcgcag ctttggcagc agcaactata tcctcatcac tggcttcaag attaccgaat
    33781 ctaagatttt cttttattgt accttcatat aaccaagcat cttgcaacac catgccaaat
    33841 tgttgccgga aatcttggcg tgataggtgt ctaatatcgt gaccatctac ggtgatagca
    33901 ccttcactaa catcgtagaa tctcatgagt aagtttataa gtgttgtctt acctgcacct
    33961 gttggcccta caattgcaat catttgccct ggttctacgt ctaagttaaa gtcgcggata
    34021 agtggcctat ccggagaata gtgaaaatcg acaccatgaa agctgacttg accggttaag
    34081 tcatggctca atttttcagt gacttgattg gcttcctctt cttcatctaa gacttcaaaa
    34141 atacgttcta aggaagattt tgcactttgc aaaacacctg ataattgtgt aatggtttga
    34201 acgggttgac taatttgcca aacatactgc acaaatgcct gcatgttacc aattgttaag
    34261 gtaccagcaa taacttgtaa accgccaaca aatgcaatga taaggtaaat aaaatcagag
    34321 ataccattca atactggcat cataattcct gaaatgaagc tagctttgaa acctacatga
    34381 cgtagattat ccgttatatc acgaaattct tgactagacg cctcttctct accatataat
    34441 tttatgatat tgaaaccagt taatttctct tgtacaaaac cattcagttc tcccagaatc
    34501 tttgcctgtt ctttaaagta aggttgggat ctttttagta tcgcttgtgc actaaaataa
    34561 gttacaggaa tacaagcaat gataatcatg gctaatggta catttagata aagtaccata
    34621 acgactacta aaataatact taaaaaagca ttgataatct gtaaaaagct ctgttgtaaa
    34681 gcatttgaga ccgtttcaac atcacttgta aaacgtccca acatatctcc aaattggtgc
    34741 ttatcaaaat acgatacagg aactttattg attttatgat tcaaatcatg acggatatca
    34801 cggatacttt tttgaacgac agtagtcata aaataatttg agccataaga acctaattca
    34861 tagaaaacac ctctgaaaaa ataaataatc aaaataccag caatataagg aacgttcaat
    34921 tctgccccag aaactccctt agccatatca acaaggttag cagtcaactc tgttatcgct
    34981 aaccctaaaa taaaaggctc cagaacactc ataaaactag ataaaacttt caaaaaaatc
    35041 gctaagaaaa gagtagcttt gtagcgagtt aggtaagacc ataatctcaa aaaaacagat
    35101 ttttttctca tacaatcact ctccttctga taagttttgg ttcttcagtt gtgactcagc
    35161 gatttcacga taaatagcat tattttctat taattgagcg tgggtaccac gaccgacaat
    35221 ttcgccttca tcaaggacaa taatctgatc agcatccata atcgtaccca ccctttgagc
    35281 aactatcaaa actgtagaat caccggttac ttcttttaga cgcgctctta aagtagcgtc
    35341 tgtcttataa tcgagagcag aaaatgaatc atcaaaaata tataaatctg gatctttaac
    35401 aacagcccta gcaatagata accgttgttt ttgaccccca gaaagattgc tcccaccttc
    35461 agctaaatgc gtttcaaagg cttcttggtg actctcaata aactctttag cttgagaaat
    35521 atcaaccgct tgtctaagat catcaatagt agcatcagct tttccatatt ttaaattctc
    35581 tcctattgtc cctgtaaata aaagagcttt ttgggggata aatccaatct tttggcgaag
    35641 tgatttaagg ttataatctc ttacatcaac tccatctact aagatttttc caagtgtcac
    35701 atcataaaaa cgtggaatca aattaacaag agaagatttt cctgaacctg ttgaaccaat
    35761 aaaagcaatt gtttctccag gcttagcttt aaaagaaata tcatgcaaaa cgggactctc
    35821 tgtttctcct ggataagcga atgttacatt atcaaattct aaatgccctt taagtttcgt
    35881 atccgtaaca ccttcggcat taggattgat agagattggc atatccatca cctctctaat
    35941 acgatgactt gataccacca tacgaggata catagtaaaa agattggcaa atagcaaaaa
    36001 tgaaaagaga gcatggaagc tatattcgat aaaagcaact aaatccccta ttttaatagc
    36061 acctctttgt aagggatcca aagcaaacca aacgatagcc acaatcattg caataataat
    36121 ttgaacgaaa agtggttctg ttagccctgt taatttaaaa agaccagttg aagtatctgt
    36181 gtaacgttgg ttagcgactt gaaatttttg tgattgaaaa ttctctcttg caaaggctct
    36241 aacaacgcgt aaccctgtta aattttcacg aacatattga ttgattttat caagcatagt
    36301 ctgttgtctt tcagataaag gttttgtttt tatagctaca tataaaacga ctcctaccaa
    36361 aagaggcatc gcaaccgcta caagccaagc caaagatgga ctcgtaatta gtatcataac
    36421 cacgctaaaa atcattacca taggagttac taggcctaaa cgtaaagaca tttcagcaaa
    36481 ttgcatcaaa acaaaagtat cgcttgtcat acgtgtcact agtgaagata cacctatctg
    36541 ttcatattca tgatgggagt attcttgaag cttagcatac atatcattac gcatatctct
    36601 aatcattgtt gtcgttaagc gactagatgc gtaagccatc gtaatacgcc caataatacc
    36661 tagtacaaca aatataaaca tgatgaacgt ccacagataa actccagtcc gatcaccttt
    36721 tgtaacgcca ttatcaatca tacccgctaa ggctgtgggc attcctagga taaccgtcac
    36781 aaaaagcata gctcctagta aatcaagcca taaccaattg gggtaccttt tcaaataaga
    36841 ccaaatatat gccatatatg tctccttttc tttcctctta tctcaaaata attaatccat
    36901 gttcactaat ccactttcaa cttataaatc tttaatcatc caaatatcca tagctgtatg
    36961 accttgatca tttgctattg gttgcgataa ggcacaatat cccatatgct tataaaccgc
    37021 agttgcccta ctcaaagtac tggctgtctc taaataaagt tgtctatacc caatttttcg
    37081 agcttctact tcaatcattt tcactaaatc agtagcaagc cccttaccac ggaaacgttc
    37141 tgcaatgtac accttctgca tctctgcaat tagatttttc agcggaccaa agccgccaca
    37201 gccaataatc tcatctctct cctcaatgac aaagaatcct gacttctcta ttttttcgta
    37261 gtatgaggtc aaatgatcta agtgagggtc tgaatatgct gtatcaggtt tatctaaatc
    37321 ataggcgcgt aaactttgtc gaattaattg tgcaactgct tggtcatcat ttttaataat
    37381 cggacggata atcatgtgtc taactcacta aggtactcat aacggtcata cttttctaga
    37441 agtgcttcat tttttgcatc taattccttt tgtaaatcag ataaccttgt aaaatcacta
    37501 ccacatgttt gcatatcatt ttctatacga gtgatagtat tttccaatat cataatatcg
    37561 tcttcaattg tcgcccattc ttgtttttca aagtaagaca ttcttttacg actttgcttt
    37621 tcacgacttg tcttggtact ctctttttta ctgataactt cattattttg ctcattaaat
    37681 gctttttcat ctaaataatc agtataatta ccaaaaaatt cacggatatc gttatcttca
    37741 aacgcaataa ttttattagc cactttatct aaaaagtaac gatcgtgact aactgtaatc
    37801 acaggaccac caaagccttg taaaaaattt tcaagaacag ttaatgtagc aatatctaag
    37861 tcatttgtcg gctcatcaag tagtaacaca ttaggctttt caatcaggat ttttaaaagg
    37921 taaagtcttt ttttctcacc acctgataat tttgcaattt gtgttccatg tgtcgaacgt
    37981 ggaaagagaa attgttccaa tagttctgtc acacttgttg taccgacact agttttaacc
    38041 tcatctgcaa cctcttgcaa ataattaata acacgttttg agccatccat attatgaagt
    38101 tgttgtgaaa agtaacctac acgtatcgtt tcaccaatag agacattacc cgaatccggc
    38161 tgtaaatctt gaacaattaa attaagtaag gttgactttc caacaccatt atctccaacg
    38221 atgccaatac ggtctttatt ttgaattaac aaattaaagt ctttcaagat agatttatct
    38281 gggtaagaaa aagagacatt ttcaaaatta ataacctttt tcccaattcg acttgtttca
    38341 aatgtcattt ccaaatcgct tgtatcgctt gtttggtgta aatcgttttt tagattttga
    38401 aaacgattaa tacgagcctg ttgtttcgtt gcacgagctt gtggctgagt acgcatccaa
    38461 gctagttcct gtttataaag ctgtttcttt ttatgtaaac tagcagcatc acgctcgtct
    38521 tgttctgcac gaagtcggac ataatcctga taattgcctt gatattctgt aatctgtgcc
    38581 ttatctaatt caaaaatacg tgttgcaaca ttgtctagaa aataacgatc atgagttata
    38641 aaaagcactg tctttttact atttttcaaa aaattcgtta accatgcaat agtgtcaata
    38701 tctaagtggt tagtaggttc gtctaagagc aataaatctg catcatttaa taatacttgc
    38761 gctaattgaa cacgtcttcg taatcctcct gataattcac caaccgacaa ctgcaaatca
    38821 gtaataccta atttggataa tactgttttg acttcgctct caatagacca agcatctaaa
    38881 gaatccattt ctgccattac tttctctaga cgtgattgct tactttcttc gtagtgatta
    38941 agcaataatt catattcttt aattaaagcc atctctctta agtcagaaga aagtacggtg
    39001 tccaaaattg tctgagaatc atcaaagtct ggttcttgtt ttaaataagc aatcttataa
    39061 tcattagctg atgaaaaagg ggaacgatca ccatcaaaac ctaattcacc cgaaataaca
    39121 tctaatagtg ttgtctttcc agttccattg acaccaataa tcccaatacg gtctaaacta
    39181 tggatgataa atgaaacatt actaaagacc gtcttatcac caaccgactt agtcaatcca
    39241 tctactaaaa aatcgctcat ataatcctct ctaatccatc aagtactgct taataaaatg
    39301 taaaatagcc tcttcttcat taatcagttg tcctaaaaca atggctaatt caatttgaga
    39361 gataatatct cccatctgtg gaccaggttt tattcctaat ttcttgatta aggtgccacc
    39421 attgacaact atctcatgcc tatcatggat tgctaactca ctatctagtt gttctatttt
    39481 ttcgaaatca accatcaagc ctcttgcttt gcgaatacct tccgcttgtt ttattatagt
    39541 ttttccataa cgataaagat gttctttagt caatcccatt tgttcacgaa tacgataaac
    39601 atccactata tgctcaacat ctttttgaaa gtgagttgat gtcttccatt tttttaaaaa
    39661 agtctttgtg tgttcaactt ccatactcaa gatgagtgat gcccaagctt gttctgatgt
    39721 ctcaaataaa aaatctttat ctaactggga caaaaaggcg cttaattgta attccctatt
    39781 ttttaagcat ggtagataat taaaagcatg actatctatt agtgccagca ttcctttacg
    39841 ccaataaggt gctaatagaa gtttatcaaa ttcaataaaa gaacgctcaa cagatatttt
    39901 ctccaataat gacgcgtgct ttttcattgc ctcaaaagtt gttgtttcga tatcaaaatt
    39961 tagactagca gaaaaacgca aacctcgcat aattcgaaga gcatcttcat taaaacgctc
    40021 actggcgagt cctactgctc taagaaggtg attatctaaa tcatccaatc catgaaagag
    40081 atcaataact tctccatctt catttaaagc aaaggcatta actgtgaaat ctcgacgttt
    40141 tagatcttct tctagcgacc taacaaaatt gacctgactc ggacgcctat aatcaacata
    40201 aacctcctca gtcctaaaag tggtaatctc atattcacca cctttttcca atactaagac
    40261 tgttccatgt tcaataccaa catcaactgt ccttttaaaa atctgctttg tttcttctgg
    40321 ataagatgac gttgctatat caacatcatg aattggacgg tcaagtaaaa catcccgaac
    40381 actaccacct acaaagtatg cttcataacc ggcttttttt atctttttta atattggtaa
    40441 agccttctga aactcagaag gcaaataatt taatctcatc atactattcc aaatctaaac
    40501 gaactaaagt cgcattatta tttcttataa atggattcag aaacaaaaag aactgcctaa
    40561 gggacactcc ctacctactc aaattatcaa aaaagaatct cctaacttct tctttgatat
    40621 ctgcaataag gatcaacctc caagcaactc tttttatcac acatcaattt ttgggggtaa
    40681 taccaaaggc aaccgcacct tcaccaagat gagttgcaat aacgccacca aatgatacaa
    40741 tttctaaatc gtctttaaga ccagctttag ctaacaaatt atataattgt tctgccttat
    40801 cttgtgctct agagtgaata atagcaatat catattcacc atctgctgtc atttctttga
    40861 caatctcagc caagcgtttt aaggcttttt tctctgtacg cactttctca tagacaacga
    40921 ttttaccttc ttcattaaaa tgtaagaccg gcttgatact taaaaggtta ccaatgatag
    40981 ctgaaccgtt tgacaatcta ccacctttta ctagatgatt caaatcatta acaacaataa
    41041 atccttcaat tttttcaatt tgtgattgta tcttgttaac tatgacatca aaatccatcc
    41101 cttcacgtga acacatcaaa gcatttctaa ctaaattgcc ctgtggtgct gaagttattt
    41161 tagtatccgg aaaagcaatt gtcaaatttg gatgttcttc aataagaaac tgaatattct
    41221 gccagaaacc tgatatcccc gctgcaatga ataaaccaag gacgtgtgta tacccttctt
    41281 tctccaattg acataagaga tcatctagtt cagctaagct aggctgactt gttttaggta
    41341 attcttttga tgccgctagc ttatcataat attggtctaa tgtcagattt tgtccctcaa
    41401 tataagtttt accatcaatg ataattggaa tatctaaaac atagacatct tcatgatttt
    41461 cgatagcctc aggcaagtaa gctgaggtat ccgtaataag tgctaatttc attaactatt
    41521 aaactccaat gttactcctg gtctatctaa agcaatttct gtaacttcat aagtcagacg
    41581 tttaacgatt tctaaaagag gagctgctag attctcaact tcatcttgac taaactcact
    41641 aggctcattg ataaggcgcc cttggatatg agccatttgt gaaatgacac cagaaataac
    41701 aaactcatca cgtacaataa caaattgtaa cactgctaca atgctcgtta cttttgctgc
    41761 ttcatctcta ttagctaatt ggaaagtaac ttcaaaattt gtttttgggg taccattttc
    41821 ctcttcccat tctaaattgc gcgcatcata gtggtattga ttgacaaatt cttgttcacg
    41881 aatgacttcc atgtattttc tccttgatag acaatatgta ttattatagc ataaatttgt
    41941 agattcttaa agcaggtggg aatctagaac ttcttcttag gatgtctatc aatcacttct
    42001 tgattgttac tcaaggctgc taaacctttt tctgtaagtg ccaaccctct gacaacaaac
    42061 aacttcccta attcctcttc tgtaaaagca atagctattg cctgatctaa atcagagcgt
    42121 ttcatctttg acaagccttt tacgtttttt tcttttaaga atagttttaa aagtggagca
    42181 gataagtgat ctaaagaatc aagcggtgac tccattctta caaatccatt ttttattaaa
    42241 aattctaggt gttcttttcc atttatacca taaatgtatt caaaatattt tgaaaaactt
    42301 gtttcagttg tgaaagttcc aaactgtata cgccataata agataatatc tccaggcaag
    42361 agtccttcac tagtttcttc catatttttc ctaggaacaa ctttactaaa attaatttca
    42421 gccaagtctc tttcaggtga gatatagggg atattaggat aatttttgta aatttcttca
    42481 atagttttca tagtcatatt gtagcaaaaa agcgcttata aaaacaagc
//
LOCUS       DATRMA010000009        39775 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.009, whole genome shotgun sequence.
ACCESSION   DATRMA010000009 DATRMA010000000
VERSION     DATRMA010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 39775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39775
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.009"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            409..588
                     /locus_tag="VBC28_000438"
     CDS             409..588
                     /locus_tag="VBC28_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000965654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Paratox"
                     /protein_id="HEO6814440.1"
                     /translation="MLYIDEFKEAIEKGYISSDTVMVVRKNGKIFDYVLPHEKVREEE
                     VVTVERVEDVMRELE"
     gene            994..1191
                     /locus_tag="VBC28_000439"
     CDS             994..1191
                     /locus_tag="VBC28_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6814441.1"
                     /translation="MSEKAILLIILIAFVIIAIIKIALLKLYMNTIKPKVDNEGNIIT
                     KPVKDDLTPEEREEREFDKDW"
     gene            complement(1235..2167)
                     /locus_tag="VBC28_000440"
     CDS             complement(1235..2167)
                     /locus_tag="VBC28_000440"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HEO6814442.1"
                     /translation="MVSLKTEIAGFSFDNCLMNAAGIYCMTKEELLAIENSEAGSFVT
                     KTGTLEAREGNPQPRYADTDWGSINSMGLPNKGIDYYLDFVTELQDQDNSKNHVLSLV
                     GLSPEETHIILKKVENSSYNGLIELNLSCPNVPGKPQIAYDFEMTDLILSEIFSYYQK
                     PLGIKLPPYFDIVHFDQAATIFNKYPLAFINCVNSIGNGLVIDDETVVIKPKNGFGGI
                     GGDFIKPTALANVHAFYKRLNPSIKIIGTGGVKNGRDAFEHILCGASMVQIGTALQKE
                     GPEIFQRVSRELKEIMADKGYQSLEDFRGQLNYL"
     gene            complement(2354..3589)
                     /locus_tag="VBC28_000441"
     CDS             complement(2354..3589)
                     /locus_tag="VBC28_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001185395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyltransferase"
                     /protein_id="HEO6814443.1"
                     /translation="MALKELTAKEFESYSGNYDLQSFMQTPEMAKLLKKRGYDITYMG
                     YQIDGKMEIISIVYTIPMTGGLHMEVNSGPAHSNSKYLKHFYKELQNYAKSQGALELL
                     IKPYDTYQEFTGEGKPKGAPNTYLIDDLTSIGYHHDGLHIGYPGGEPDWHYVKNLEGI
                     TPQNLLKSFSKKGRPLVKKAMSFGIKIRVLKREELHIFKDITSSTSDRRDYMDKSLDY
                     YQDFYDSFGDKAEFVIATLNFREYDHNLQLNAKKLEEQITVLDNRHQNNTDSAKYHRQ
                     RTELVNQLASLDKRRKEVEPFIQKFGNQDVVLAGSLFIYSPKETVYLFSGSYTEFNKF
                     YAPAVLQEYVMQEALKRQSTFYNFLGIQGNFDGSDGVLRFKQNFNGYIVRKMGTFRYY
                     PNPLKYKSIQLLKKILRRT"
     gene            complement(3608..4819)
                     /locus_tag="VBC28_000442"
     CDS             complement(3608..4819)
                     /locus_tag="VBC28_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyltransferase"
                     /protein_id="HEO6814444.1"
                     /translation="MYREITAVEHDRFVSESNQTNLLQSSNWPKVKDNWGSQLLGFFD
                     GETQIASASILIKSLPLGFSMLYIPRGPIMDYSNLDIVTKVLKDLKAFGKKQRALFIK
                     CDPLIYLKMVNAKDFENSPDEKEGLIAIDHLQRAGADWTGRTTDLAHTIQPRFQANLY
                     ANQFGLDKMSKKTRQAIRTSKNKGVDIQFGSHELLEDFAELMKKTEDRKGINLRGIDY
                     YQKLLDTYPNNSYITMASLDVAKRLEKIEKECQIAQSERIKSLELNREKKVKQHQGTI
                     DRLNKEIDFLKEAQKAYDRDIIPLAATLTLEFGNTSENIYAGMDDYFKSYSAPIYTWF
                     ETAQRAFERGNIWQNMGGIENDLSGGLYHFKSKFEPIIEEFIGEFNIPVNRLLYKASN
                     YVYALRKKRNS"
     gene            complement(4832..6067)
                     /locus_tag="VBC28_000443"
     CDS             complement(4832..6067)
                     /locus_tag="VBC28_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyltransferase"
                     /protein_id="HEO6814445.1"
                     /translation="MGNKLMYHVTVGISEKEYDAFAIASSQTNLLHSSKWAQVKSNWQ
                     NERLGFYKDDQLVAVASILIKSLPLGFTMLYIPRGPIMDYSNKELVNFVLKTLKNFGR
                     KKRAVFAKFDPALLLRQYHLKEENVAEEIDESRQAIDNLKSAGAQWIGPTKAISETIQ
                     PRFQANIYTKANIEENFPKHTKRLIKDAKHRGVQIYRANIDDLPKFATVVALTENRKG
                     VALRNENYFHQLMTIYGEDAYLYLAKVNLPKRLAQFKEQLLQIQKDLSETPSHQKSRL
                     TRLNQQEASVKQYILEFQEFSKKYPDEPVIAGILSIRFGNVLEMLYAGMDDSFRKFYP
                     QYLLNARVFEDAFKNDIVSANLGGVEGSLNDGLTKFKSNFNPMFEEYIGEFNLAINPL
                     LYKLANLAYTIRKKQRHSH"
     gene            complement(6052..6864)
                     /gene="yidA"
                     /locus_tag="VBC28_000444"
     CDS             complement(6052..6864)
                     /gene="yidA"
                     /locus_tag="VBC28_000444"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HEO6814446.1"
                     /translation="MTSIKLVAVDIDGTLLNSKREITPEVAKAVQEAKSKGVKIVIAT
                     GRPIIGVQDLLEELKLNEEGDYVITFNGGLVQDTATGDDIIKETLTYEDYLDFELLAR
                     KLGVHMHAITKEGIYTANRDIGKYTIHEVTLVNMPLFYRTPEEMGDKEIIKLMMIDQP
                     DILDAAIAKIPKKVLDNYTIVKSTPFYLEILPKNVNKGTALLHLAEKMGLTVDQTMAI
                     GDEENDRAMLEVVGNPVVMQNGNPELKKIAKYITKSNEESGVAYALREWVIN"
     gene            complement(6935..8251)
                     /locus_tag="VBC28_000445"
     CDS             complement(6935..8251)
                     /locus_tag="VBC28_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEO6814447.1"
                     /translation="MNEKVFRDPVHTYIHVNNQVIYDLINTKEFQRLRRIKQTSTTSF
                     TFHGAEHSRFSHCLGVYELARKVTEIFDEHYSDLWNKNESLLTMAAALLHDIGHGAYS
                     HTFERLFNTDHEAYTQEIITNPTTEINAILRKVAPDFPDKVASVINHSYPNKQVVQLI
                     SSQIDCDRMDYLLRDSYYTAASYGQFDLTRILRVIRPTDSGIAFARNGMHAVEDYIVS
                     RFQMYMQVYFHPASRAMELLLQNLLKRARFLFDTHRDFFEQTSPNLIPFFTDQYDLQD
                     YLALDDGVMNTYFQSWMQADDNILADLANRFINRKVFKSITFEESDKENLVKMKELVS
                     QVGFDPDYYTGVHANFDLPYDVYRPEHSNPRTEIQIIQKNGQLAELSSLSPIVKALTG
                     SNYGDQRFYFPKEMLTLDSLFSSTKEEFQSYITNEHLTLTKDNSHS"
     gene            8315..8713
                     /locus_tag="VBC28_000446"
     CDS             8315..8713
                     /locus_tag="VBC28_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001883692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HEO6814448.1"
                     /translation="MREVMKLEINNNIQIDNETEMIHEIHDCQFIEKGSYVYLNYINA
                     EGERVVIKANHEELLMTRFSNPKSVMRFHRETPALVNIPTPLGVQHLITETSHYQFDL
                     SQQRLHINYVLKQTETGDCFANYELRIQWY"
     gene            9057..11741
                     /locus_tag="VBC28_000447"
     CDS             9057..11741
                     /locus_tag="VBC28_000447"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HEO6814449.1"
                     /translation="MLKEQKKSLFYTQGQEEVLTSLESSREGLSTTEAKNRLEMYGRN
                     ELEEGKKRSLIAKFFDQFKDLMIIILLVAAALSVITEGMHGLTDALIILAVVILNAAF
                     GVYQEGQAEAAIEALKDMSSPIARVRRDGHTIEVDSKELVPGDLVMLEAGDVVPADLR
                     LLEAASLKIEEAALTGESVPVEKDISQVVAEDAGIGDRVNMAYQNSNVTYGRGYGVVT
                     NTGMYTEVGKIADMLANADESETPLKQSLVQLSKLLTYLIVIIAVITFLVGIFVRKEG
                     WIEGLMTSVALAVAAIPEGLPAIVTIVLSMGTKTLAKRNSIVRKLPAVETLGSTEIIA
                     SDKTGTLTMNQMTVEKVYTNGVLQSSSEEISVDNNTLRIMNFSNDTKIDPSGKLIGDP
                     TETALVQFGLDKNFDVREVLKNEPRVAELPFDSDRKLMSTIHKESDGRYFIAVKGAPD
                     QLLKRVTKIEDNGLVRDITAEDKEAILNTNKELAKQALRVLMMAYKYETQIPTLETDI
                     VESDLVFSGLVGMIDPERPEAAEAVRVAKEAGIRPIMITGDHQDTAEAIAKRLGIIDA
                     NDTEDHVFTGAELNELSDEEFQKVFKQYSVYARVSPEHKVRIVKAWQNDGKVVAMTGD
                     GVNDAPSLKTADIGIGMGITGTEVSKGASDMVLADDNFATIIVAVEEGRKVFSNIQKS
                     IQYLLSANMAEVFTIFFATLLGWDVLAPVHLLWINLVTDTLPAIALGVEPAEPGVMTH
                     KPRGRQSNFFDGGVMGAIIYQGILQTILVLGVYGWALMYPEHAGYRMIHADALTMAFA
                     TLGLIQLVHAFNVKSVYQSIFTVGAFKNRTFNWSIPVAFILLMVTIVVPGFNKLFHVT
                     HLSSTQWLTVVIGSLLMVVLTEIVKFIQRKLGQDEKAI"
     gene            complement(11786..12646)
                     /locus_tag="VBC28_000448"
     CDS             complement(11786..12646)
                     /locus_tag="VBC28_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEO6814450.1"
                     /translation="MTKLAIMSDLHIDLNHFSTFETKTLINLLKKEKIDHLHIAGDIS
                     NHFTKDTLPFINNLKKHIKLSYNLGNHDMLDLTETEIQRLDFQTYRFDKKMLLAFHGW
                     YDYSFSNNRDIKDVEKLKKTFWFDRRLKRPNNDVTIQASILKRLDEILAKVDSSNIII
                     AMHFVPHKQFTMTHPRFSPFNAFLGSQAYHDLFQKYHIKDVVFGHAHRSFGDVKIGET
                     TYHSRPLGYIREWNLTIDFVNQNPNHNPNLTWNLSKRYNAVKHLDSFENYRKKYFEDE
                     LRNSMTIFDC"
     gene            12798..14729
                     /locus_tag="VBC28_000449"
     CDS             12798..14729
                     /locus_tag="VBC28_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1,6-bisphosphatase"
                     /protein_id="HEO6814451.1"
                     /translation="MSNFYKLLKEKFPRKEDIVTEMINLEAICQLPKGTEYFISDLHG
                     EYDAVDYLLRTGAGSIRAKLLDCFDWQKIVAVDLDDFCILLYYPKEKLAFDKMNLSAS
                     AYKTKLWEMIPLQIQVLKYFSSKYTKSKVRKQLSGKFAYIIEELLAEIDRNPEKKSYF
                     DTIIEKLFELDQVEDLIIVLSQTIQVLIIDHLHVVGDIYDRGRYPDRILNRLMAFPNL
                     DIQWGNHDVTWMGAASGSYLCMVNVIRIAARYNNITLIEDRYGINLRRLVDYSRRYYE
                     PLPSFVPILDGEEMTHPDELDLLNMIQQATAILQFKLEAQLIDRRPEFQMHNRQLINQ
                     VNYKDLSISIKEVVHQLKDFNSRCIDSKNPSRLTSEEEELLQQLMIAFQTSESLKKHI
                     DFLFEKGSMYLTYNDNLLFHGCIPMHSNGDFKSFKIAGKTYGGRDLLDLFESQIRLAY
                     ARPEKHDDLATDIIWYLWCGENSSLFGKNAMTTFERYYVSDKVTHQERKNPYFKLRDK
                     DDICTALLQEFDLPKFGHIVNGHTPVKEKNGEQPIKANGKMLVIDGGFAKGYQKNTGL
                     AGYTLIYNSYGIQLISHLPFTSIEEVLSGTNYIIDTKRLVEEAKDRILVKDTTIGQKL
                     TKEIKDLDHLYRHFQEYDD"
     gene            14819..15943
                     /gene="queG"
                     /locus_tag="VBC28_000450"
     CDS             14819..15943
                     /gene="queG"
                     /locus_tag="VBC28_000450"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HEO6814452.1"
                     /translation="MDIKAEIQKLAKEIGISKIGFTTADNFDYLEKSLRASVEEGRNS
                     GFEHKVIEDRIYPERLLESAKTIISIGVAYPHKLPQQPQKTSYKRGKITPNSWGLDYH
                     YVVGEKLDRLSKGIEELCRDFPLQQKAMVDTGALVDTAVAQRAGIGFIGKNGLVISKE
                     YGSYMFLGELITNLEIEPDKPVDYDCGDCRRCLDACPTSCLIGDGSMNAKRCLSFQTQ
                     DKGMMDIEFRKKIKTVIYGCDICQICCPYNKGINNPLATEIDPELAQPELIPFLSLSN
                     GQFKEKFGMIAGSWRGKNILQRNAIIALANAHDKTAVVKLIEIIDKNNNPIHTATAIW
                     ALGEIVKKPNDEILEFMSNLTLKDEDSRKELELIRHKWQF"
     gene            16012..17110
                     /gene="prfB"
                     /locus_tag="VBC28_000451"
     CDS             join(16012..16083,16085..17110)
                     /gene="prfB"
                     /locus_tag="VBC28_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889043.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEO6814453.1"
                     /translation="MEVAEIRQKIVENKEKLTSFRRSLDLDRLEEEIALLENQMTEPD
                     FWNDNIAAQKTSQELNELKGKYDTFHNMQELSDETELLLEMLDEDDSLKEELEENLMQ
                     LDKIMGAYEMTLLLSEPYDHNNAILEIHPGSGGTEAQDWGDLLLRMYTRFGNANGFKV
                     EVLDYQAGDEAGIKSVTLSFEGPNAYGLLKSEMGVHRLVRISPFDSAKRRHTSFASVE
                     VMPELDDTIEVEVRDDDIKMDTFRSGGAGGQNVNKVSTGVRLTHIPTGIVVSSTVDRT
                     QYGNRDRAMKMLQAKLYQLEQEKKAQEVDALKGDKKEITWGSQIRSYVFTPYTMVKDH
                     RTNFELAQVDKVMDGEINGFIDAYLKWRIED"
     gene            17129..17821
                     /gene="ftsE"
                     /locus_tag="VBC28_000452"
     CDS             17129..17821
                     /gene="ftsE"
                     /locus_tag="VBC28_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEO6814454.1"
                     /translation="MALIEMSGVTKKYRRSTTALRNLNLSIQQGEFVYLVGPSGAGKS
                     SLIRLLYREEKLSSGRLKVGEFNLNKLKRRQIPILRRSIGVVFQDYKLLPTKTVYENV
                     AFAMQVIGAKRRHIKKRVPEVLELVGLKHKMRSFPTQLSGGEQQRVAIARAIVNNPKL
                     LIADEPTGNLDPEIAWEIMHLLERINLQGTTVLMATHNSQIVNTLRHRVIEIEAGSVI
                     RDEEKGEYGYHD"
     gene            17805..18734
                     /locus_tag="VBC28_000453"
     CDS             17805..18734
                     /locus_tag="VBC28_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6814455.1"
                     /translation="MVIMINFFRHFWESLKNLKRNFWMTFASVTSVTITLLLVGLFSS
                     VLLNVEKLTTDVSGNFTISAFLNVDSTDAQKQVKDKDGKLKDNPDYHKVYDKIKRISG
                     VEKVTYSSKAEQLKEVQKEYGSDVIDDTYKDALLDVYVVGTSSAKVSKSVSEAIGRIE
                     GVDYTKEPIDSTKLSNLTDNIRIWGFGGVALLIVLAIFLISNTIRMSIMSRRTDIEIM
                     RLVGAKNSYIRGPFFFEGAWVGILGAIVPSLIFYFGYQFVFNKFNPKFETSHVSLYPM
                     DIMVPAIIGGMVIIGIIIGSLGSVLSMRRYLKI"
     gene            complement(18787..19497)
                     /locus_tag="VBC28_000454"
     CDS             complement(18787..19497)
                     /locus_tag="VBC28_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001022568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEO6814456.1"
                     /translation="MNIKRHSIFLWLTTSILAILMAVGMTIYFNTYQASPLARRIAKT
                     ATNHKNYLLFRSKGKVKANIIFYQGALVEEEAYSQLARDLADKGDNTYILKTPLNLPV
                     LSPHKAKTIINQNHLTNVYLAGHSLGGVVASQNAKVAPVRGLILLASYPSRKSDLSHK
                     KLRVLSITASNDHILNWEKYEEAKKRLPNSSTFRTIVGGNHSRFGNYGHQKGDGKATL
                     SHKSSEKQLATFISNFIH"
     gene            complement(19494..20192)
                     /locus_tag="VBC28_000455"
     CDS             complement(19494..20192)
                     /locus_tag="VBC28_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001907086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEO6814457.1"
                     /translation="MPFIFRHSFFNKVLIFWYTIIMKIYKTINHIAGENTYYLVNDQA
                     VILIDPGSNGQEIIAKIKSFEKPLVAILLTHTHYDHIFSLDLVRDTFDNPPVYVSEKE
                     AAWLSSPDDNLSGLGRHDDIINVIARPAENFFKLKQPYQLNGFEFTVLPTPGHSWGGV
                     SFVFHSDELVVTGDALFRETIGRTDLPTSNFEDLITGIRQELFTLPSHYSVHPGHGMN
                     TTIGHEKNFNPFFQ"
     gene            20360..21124
                     /locus_tag="VBC28_000456"
     CDS             20360..21124
                     /locus_tag="VBC28_000456"
                     /EC_number="1.1.1.304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-acetoin forming diacetyl reductase"
                     /protein_id="HEO6814458.1"
                     /translation="MSKVAIVTGAGQGIGFAIAKRLHADGFKIGVLDYNEETAQAAVD
                     KLSPEDAVAVVADVSKRDQVFDAFQKVVDTFGDLNVVVNNAGVAPTTPLDTITEEQFE
                     KAFAINVGGTIWGSQAAQKHFRELGHGGKIINATSQAGCEGNPNLTVYGGTKFAVRGI
                     TQTLAKDLASEGITVNAYAPGIVKTPMMFDIAHEVGKNAGKDDEWGMEQFAKDITLKR
                     LSEPEDVANAVGFLAGDDSNYITGQTIVVDGGMVFH"
     gene            21232..23739
                     /locus_tag="VBC28_000457"
     CDS             21232..23739
                     /locus_tag="VBC28_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DnaQ family
                     exonuclease/ATP-dependent helicase"
                     /protein_id="HEO6814459.1"
                     /translation="MFCFIDIACYNRLTMTQKKLRKYAVVDLEATGAGPNASIIQVGI
                     VIIQGNKIIDSYETDVNPHESLDEHIVHLTGITDKQLAKAPDFGQVAHHIYQLIEDCI
                     FVAHNVKFDANLLAEQLFLEGCELRTPRIDTVELSQVFYPCLEKYSLGALAESLNIEL
                     TDAHTAIADARATAQLFIKLKAKISSLPKEVLETILTFADNLLFESYLLIEEAYQEAD
                     FVNPKEYYFWQGLVLKKEKAVGKPKKLSSDFQVNMALLGMDARPKQVVFADLVKAHFN
                     DQTTTFLEAQPGLGKTYGYLLPLLDQSQKQQIIVSVPTKILQDQIMAKEIKHIQELFH
                     IPCHSIKGPRNYLKLDAFYKSLQVQDRNRLINRFKMQLLVWLTETTTGDLDEIKQKQR
                     LESYFDQLKHDGEVTQSSLFYDLDFWKRSYDKVAQSQLVIINHAYFLERVQDDKDFAK
                     GKVLVFDEAQKLVLGLENFSRGQLDISHQLQVIQKIIDSSIPLLQKRLLESISYELSH
                     AVELFYRHNSFEFSETWLKRLKNSINALEVVGLDELQTFFTATYTNYWFETDKVNEKR
                     LTILRGAREDFLKFSKFLPPTKKTYMISATLQISPKVYLSDLLGGFSSISTEKIAHEK
                     NANQKVWIDTSMPNILDLSPEQYAYEIAKRLQDIMTLKQPTLVLLTSKQTMFMVSDYL
                     DKWEIKHLTQDKNGLAYNVKKRFDRGESNLLLGTGSFWEGVDFVHRDRLIEVITRLPF
                     DTPKDYFIQKLSQSLTKEGKNFFYDYSLPMTVLKLKQALGRTTRREEQKSAVIILDSR
                     LVIKSYGQTIMHSLGRDFEISKEKINKVLTEMAKFLI"
     gene            23825..25018
                     /locus_tag="VBC28_000458"
     CDS             23825..25018
                     /locus_tag="VBC28_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEO6814460.1"
                     /translation="MTYLSERVLNMEESVTLAAGAKARELRVQGRDILSLTLGEPDFA
                     TPKNIQQAAIEAITDGRASFYTPSSGLPELKSAINAYFERFYGYSLKPNQVVVGTGAK
                     FILYTFFMTVLNPGDEVIIPTPYWVSYADQIKMAEGKPVFVTAKEVNHFKVTVEQLEA
                     VRTDKTKVILLNSPSNPTGMIYKAEELEAIGNWAVEHDILILADDIYGRLVYNGNIFT
                     PISSLSESIRNQTIVINGVSKTYAMTGWRVGFAVGNHDIIAAMSKVVSQTTSNLTAVS
                     QYATIEALNGSQESFEKMRLAFEERLNIIYPLLCQVPGFEVVKPQGAFYLFPNVTKAM
                     EMKGYTDVTAFTDAILEEVGLALVTGAGFGAPENVRLSYATDLETLKEAVRRLHVFMG
                     SNEIN"
     gene            25039..26385
                     /gene="asnS"
                     /locus_tag="VBC28_000459"
     CDS             25039..26385
                     /gene="asnS"
                     /locus_tag="VBC28_000459"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HEO6814461.1"
                     /translation="MSKKLISIVDVKDYVGQEVTIGAWVANKSGKGKIAFVQLRDGSA
                     FFQGVAFKPNFIEKYGEESGLEKFDVIKRLNQETSVYVTGIVKEDERSKFGYELDITD
                     LEVIGESHEYPITPKEHGTDFLMDNRHLWLRSRKQMAVMQIRNAIIYSTYEFFDQNGF
                     IKFDSPILSENAAEDSTELFETDYFGKPAFLSQSGQLYLEAGAMALGRVFDFGPVFRA
                     EKSKTRRHLTEFWMMDAEYSFLSHEESLDLQEAYVKALIQGVLDRAPQALDILERDVE
                     ALKRYIAEPFKRVSYDDAITLLQEHEADEDTDYEHLEHGDDFGSPHETWISNYFGVPT
                     FVVNYPASFKAFYMKPVPGNPERVLCADLLAPEGYGEIIGGSMREDDYDALVAKMDEL
                     GMDKSEYDFYLDLRKYGSVPHGGFGIGIERMVTFVAGTKHIREAIPFPRMLHRIKP"
     gene            complement(26425..26982)
                     /locus_tag="VBC28_000460"
     CDS             complement(26425..26982)
                     /locus_tag="VBC28_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEO6814462.1"
                     /translation="MTKSPARLISFISIAIAINLVGANLALFLRLPIYLDTIGTLLIA
                     VILGPWYAASTAFLSALINWMTTDIFSLYYSPVAIVVAIITGILIKRNCKPSSLLWKS
                     LIISLPGTIIASVITVILFKGITSSGSSIIAQFLHGIGLDMTSSLILVQVGTDYMDRL
                     ISLILVFSTITLLKKHSPNLINQRF"
     gene            complement(26979..27962)
                     /locus_tag="VBC28_000461"
     CDS             complement(26979..27962)
                     /locus_tag="VBC28_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001031103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HEO6814463.1"
                     /translation="MNKEKIIIDCDPGIDDTLALMYAIQHPKLEVVAITITAGNSPVE
                     LGLKNTFVTLELLNRHDIPVYVGDNLPLQREFVSAQDTHGMDGLGENNFTLAQPIIFQ
                     EESADCFLANYFEHKNDTSIIALGPLTNIARALQTNPKLGKHCKRFISMGGSFKSHGN
                     CSPVAEYNYWCDPHAAQYVFENLDKKIEMVGLDITRHIVLTPNHLSYMERINPDVSSF
                     IQKITKFYFDFHWQYEHIIGCVINDPLAIAYFVNENIATGFDSYTDVACHGIAMGQTI
                     VDQYHFYKKDANSKILTSVNTNLFWNDFMTVLLHAQNSVIIEDLEMLGLNK"
     regulatory      complement(28106..28150)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            complement(28208..28621)
                     /locus_tag="VBC28_000462"
     CDS             complement(28208..28621)
                     /locus_tag="VBC28_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEO6814464.1"
                     /translation="MLYEVTSSNTQGVDGKVYLSNGKIVETNHPLNHLPGFNPEELIA
                     LAWSTCLNATIKAILEQKGFKDLKSRVDVTCQLMKEKQVGKGFYFQVNAVASIEKLSL
                     SDSKLIVNKAHSRCPISKLISNAKTINLDTVKWSV"
     gene            28775..29665
                     /gene="rapZ"
                     /locus_tag="VBC28_000463"
     CDS             28775..29665
                     /gene="rapZ"
                     /locus_tag="VBC28_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEO6814465.1"
                     /translation="MSDEQIKLVIVTGMSGAGKTVAIQSFEDLGYFTIDNMPPTLVPK
                     FLELAAQSGDTSKIAMVVDMRSRLFFREINSILDSLEINDNINFKILFLDATDTELVS
                     RYKETRRSHPLAADGRVLDGISLERELLAPLKSMSQNVVDTSELTPRQLRKVISKEFS
                     NQDSQSSFRIEVMSFGFKYGIPLDADLVFDVRFLPNPYYKPELRDKTGLDTEVYDYVM
                     SFDESDDFYDHLLALIKPILPGYQNEGKSVLTVAIGCTGGQHRSTAFAHRLSEDLKAD
                     WTVNESHRDKNKRKETVNRS"
     gene            29662..30636
                     /locus_tag="VBC28_000464"
     CDS             29662..30636
                     /locus_tag="VBC28_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001231103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HEO6814466.1"
                     /translation="MRKPKITVIGGGTGIPVILKSLRLEDVEITAVVTVADDGGSSGE
                     LRSVMQLTPPGDLRNVLVALSDMPKFYEQIFQYRFAEGDGDFAGHPLGNLIIAGVAEM
                     QGSTYNAMQSLTQFFHTTGKIYPSSEHPLTLHAVFKDGHEVVGESQIADYKGMIDHVY
                     VTNTYNEETPTASRKVVDAILESDMIVLGPGSLFTSILPNLVIPEIKQALLETRAEVA
                     YVCNIMTQRGETEHFTDADHVEVLKRHLGQDAIDTVLVNIEKVPESYMENNHFDEYLV
                     QVEHDFSGLRKHARRVISSNFLKLEKGGAFHHGDFVVEELMNLVGTHL"
     gene            30633..31544
                     /gene="whiA"
                     /locus_tag="VBC28_000465"
     CDS             30633..31544
                     /gene="whiA"
                     /locus_tag="VBC28_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000011317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HEO6814467.1"
                     /translation="MSFTVKVKEELLGHKSENKMELSAIIKMSGSLGLANHGLNLSIT
                     TENAKIARHIYSMLEEHYHLQPEIKYHQKTNLRKNRVYTVFIEEKVDVILADLKLADA
                     FFGIETGIEHSILDNDENGRAYLRGAFLSTGTVREPDSGKYQLEIFSVYLDHAQDLAN
                     LMKKFMLDAKVIEHKHGAVTYLQKAEDIMDFLIVIDAMEARDAFEEIKMIRETRNDIN
                     RANNVETANIARTITASMKTINNIIKIMDTIGFDALPSDLRQVAQVRVAHPDYSIQQI
                     ADSLETPLSKSGVNHRLRKINKIADEL"
     gene            31559..32956
                     /locus_tag="VBC28_000466"
     CDS             31559..32956
                     /locus_tag="VBC28_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HEO6814468.1"
                     /translation="MACTTILVGKKASYDGSTMIARTEDSVNGDFTPKKLKVMTSKDQ
                     PRHYKSVLSNFEVDLPDNPLPYTSVPDALGKDGIWGEAGINSKNVAMSATETITTNSR
                     VLGADPLVSDGIGEEDILTLVLPYIQSAREGVERLGAILEKYGTYESNGIAFSDTEEI
                     WWLETIGGHHWIARRVPDDVYVTNPNQLGIDHFEFNNCDDYMCSSDLKEFIEQYHLDL
                     TYSNEHFNPRYAFGSQRDKDRHYNTPRSWAMQRFLNPEIEQDPRSLFIPWCQKPYRKI
                     TVEDIKYVLSDHYQDSVYDPYGPEGDAVSRRAFRSVGINRTSQTSILQLRPNKSLETT
                     GVQWLSYGSMPFATMVPLFTQVETVPNYFSNTTKDASTDNFYWTNRLIAALADPHFYQ
                     HEADIESYIERTMAQGHAHINGVDREVAENKEIDFQQKNQEMSDYIQKESQELLNRIL
                     FDASNLMTNRFSMGD"
     gene            33098..34618
                     /locus_tag="VBC28_000467"
     CDS             33098..34618
                     /locus_tag="VBC28_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HEO6814469.1"
                     /translation="MRKKFLLLMSFVAMFAAWQLVQVKQVWADSKLKVVTTFYPVYEF
                     TKNVVGDKADVSMLIKAGTEPHDFEPSTKNIAAIQDSNAFVYMDDNMETWAPKVAKSV
                     KSKKVTTIKGTGDMLLTKGVEEEGEEHEGHGHEGHHHELDPHVWLSPERAISVVENIR
                     NKFVKAYPKDAASFNKNADAYIAKLKELDKEYKNGLSNAKQKSFVTQHAAFGYMALDY
                     GLNQVPIAGLTPDAEPSSKRLGELAKYIKKYNINYIYFEENASNKVAKTLADEVGVKT
                     AVLSPLEGLSKKEMAAGEDYFSVMRRNLKVLKKTTDVAGKEVAPEEDKTKTVETGYFK
                     TKDVKDRKLTDYSGNWQSVYPLLQDGTLDPVWDYKAKSKKDMTAAEYKKYYTAGYKTD
                     VESIKIDGKKHQMTFVRNGKSQTFTYKYAGYKILTYKKGNRGVRYLFEAKEKDAGQFK
                     YIQFSDHGIKPNKAEHFHIFWGSESQEKLFEEMENWPTYFPAKMSGREVAQNLMSH"
     gene            complement(34731..34991)
                     /locus_tag="VBC28_000468"
     CDS             complement(34731..34991)
                     /locus_tag="VBC28_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018370011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HEO6814470.1"
                     /translation="MKKDIHPDYRPVVFLDTTTGYKFLSGSTKSTKETVEFEGETYPL
                     IRVEISSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAQ"
     gene            complement(35100..36035)
                     /locus_tag="VBC28_000469"
     CDS             complement(35100..36035)
                     /locus_tag="VBC28_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000583237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HEO6814471.1"
                     /translation="MIIFQQILDKIKEYDTIIIHRHMRPDPDALGSQIGLRDIIRHNF
                     PKKKVLATGFDEPTLAWIAKMDQVTDQDYQGALVVVTDTANTPRIDDERYKKGDFLIK
                     IDHHPNDEVYGDLSYVDTNASSASEIVTDFALSCDLLLSTSAARVLYNGIVGDTGRFL
                     YPATTSKTLKIASKLREFDFDFSAMARQMDSFPFKIAKLQGFIFEQLKIDKNGAACVT
                     LTQEDLKRFDVTDAETAAIVGVPGKIDIVESWAIFVEQSDGHYRVRLRSKSHIINEIA
                     KRHNGGGHPLASGANSYSLEENQAIYQEIQEILSL"
     gene            36301..37323
                     /locus_tag="VBC28_000470"
     CDS             36301..37323
                     /locus_tag="VBC28_000470"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HEO6814472.1"
                     /translation="MTQAVLKELAKAELHCHLDGSLSLPAIRKLANMADIILPSSDKE
                     LRKYVIAPAQTESLVDYLKTFEFIRPLLQTKEALRFAAYDVARQAALENVIYIEIRFA
                     PELSMDKGLTASDTVLAVLEGLADAQKEFNIVARALVCGMRQSSHKTTKDIIKHIVDL
                     APKGLVGFDFAGDEFSYPTDSLVDLIQEVKRSGYPMTLHAGECGCAKHIADSLNLGIK
                     RMGHVTALTGQRDLIKRFVEEDAVAEMCLTSNLQTKAASSIQSFPYQELYDAGGKITI
                     NTDNRTVSDTNLTKEYSLFVTYFGTKIEDFLVFNQNAVKASFTSDSEKDTLLHKLQEN
                     YDSYLK"
     gene            37382..37825
                     /locus_tag="VBC28_000471"
     CDS             37382..37825
                     /locus_tag="VBC28_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HEO6814473.1"
                     /translation="MALAKIVYASMTGNTEEIADIVADKLRDLGLDVEVEECTMVDAA
                     DFEDADIAIVATYTYGDGDLPDEIVDFYEDLAEVDLSGKVYGVVGSGDTFYDYFCKSV
                     DEFEAQFALTGAQKGADCVKVDLAAEDEDIENLEAFAEEIASKLN"
     gene            37902..38177
                     /locus_tag="VBC28_000472"
     CDS             37902..38177
                     /locus_tag="VBC28_000472"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEO6814474.1"
                     /translation="MELEEIRQEIDEIDQQLVSLLETRMGLILEVIAFKKKHRLPVLD
                     NNRENEVLNNVLKKVQNHQFDDVIRATFKDIMTESRVYQKENIVDGD"
     gene            38170..39366
                     /locus_tag="VBC28_000473"
     CDS             38170..39366
                     /locus_tag="VBC28_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HEO6814475.1"
                     /translation="MIKRFDNYAQYVLVLGFSLLIGGVVGAITAVFGRVLLFLTAFRS
                     DYIAYLLPFLSIVGLFIVFVYQKFGGKSVKGMGLVFEVGHGNEETIPKRLVPLVILTT
                     WLTHLFGGSAGREGVAVQIGATVSHYFQKYCRLQNASQLFLVMGMAAGFAGLFQTPLA
                     ATFFAIEVLVVGRLMVSYVLPSLIAALTANFVSHSLGLEKFSHSIATSMALTPDIILK
                     LLVLGLCFGLCGNLFAYLLAKAKLIASSRLLNPYKRIFTLGLLATFLLFIFHFGRYSG
                     LGTNLIEASFTNKNLYDYDWLLKLCLTVITLAAGYQGGEVTPLFAIGASLGVIIAPIL
                     GLPVILVAALGYTSVFGSATNTLLGPILIGGEVFGFANTPYFVIVCLVAYSISHAHTI
                     YGAQSR"
BASE COUNT        12787 a         6285 c         7819 g        12884 t
ORIGIN      
        1 ttattaataa agtaaaatac aactaaacaa atcgcgaaaa taatcaaata tttcatattt
       61 gtcttagata tgatatacta tcagtagtgg caaggggctt gagccccaaa ctactactag
      121 aaccttattt gaatctccgt ggccgttttt tctttttagg ttcttttttt attgctgtga
      181 ttatgcttgc tataccaacc aacagagttc cgattgaagt aagcaaatca gcaatttctg
      241 atattctcat atcttcctcc tttctatata ttaattataa tacatgtact atataaactc
      301 aatacttttt ttaaaaatat ttatcttttt ttgtctatca caacagaaaa atttaaaatt
      361 gtctattttt aggatttttt tacgaataga tagagaggag gataaaatat gttatacata
      421 gatgagttta aagaagcgat tgaaaaagga tatatcagca gtgatacagt gatggttgtg
      481 cgtaaaaacg gaaagatatt tgattatgtg ttaccacacg aaaaagtgag agaagaagag
      541 gttgtgacag ttgagagagt ggaagatgtt atgagagaat tggagtaatg ttcgtcgtga
      601 cataagcagc tatcaatagc ctgccccaca aaaaagcata ttatagttgg ggcgaatttg
      661 gggcataagt ttaaaactaa tctatgttaa caatgatacg acttttacgt ttactactca
      721 taacgactta tatagatatg ttttagacct tataatgcca accgaaaaac ctaactctta
      781 aagacgctgt taaataattc gtctagaaaa accttgtcat atcaatgttt attgatagcg
      841 acaaggttct tttttatatc caatactcaa taaatgacta gagagattaa aggaatttag
      901 agtttcaaat gtgttagagc atacccttga attatcttaa ctgtgatgtt ataattccta
      961 aaagataagt taaaagagag gttgcgaaag agaatgtcag aaaaagctat actattaatt
     1021 atccttattg cttttgttat tatcgctatt ataaagatag ctctgttaaa gttatatatg
     1081 aatacgataa aacctaaagt agataatgaa ggaaacatca taacaaagcc tgtaaaggat
     1141 gatttaacac ccgaggaacg tgaagaacgt gaatttgata aagattggta aaatcaatat
     1201 aaaagagcaa acaaggtttg ctcttttatt atttttaaag gtaattaagt tgtcctcgaa
     1261 aatcctccaa tgattgatag cctttatcag ccattatttc ttttaattct ctgcttacac
     1321 gctgaaaaat ttctggtcct tctttttgta aagcggttcc gatttggacc atactagctc
     1381 cacatagaat gtgttcaaag gcatcacggc cgtttttaac accacctgtt ccgataattt
     1441 taattgaagg atttaaacgt ttgtagaaag catggacgtt agctaaggcg gtaggcttga
     1501 taaaatcccc accgatacct ccaaagccat ttttaggctt tataacgaca gtttcatcat
     1561 caataacaag tccatttccg atagaattaa cacagttaat aaaagctaaa ggatatttgt
     1621 taaaaattgt agctgcttga tcaaagtgaa cgatatcaaa gtaaggtggt aatttgatgc
     1681 ccaatggctt ttgataatag ctaaagattt ctgaaaggat gaggtcagtc atttcaaaat
     1741 cataagcaat ttgaggtttt ccaggaacat ttgggcacga taaatttaac tctattaggc
     1801 cattgtaact actgttttca acctttttaa gaataatatg tgtctcttct ggtgataaac
     1861 ctactaatga taaaacgtga tttttgctat tgtcttgatc ttgtaattca gtcacgaaat
     1921 caagataata gtcaatacct ttattaggga gtcccataga attaattgat ccccagtcag
     1981 tatctgcata acgtggttga gggtttccct ctctagcttc aagggtacct gtttttgtaa
     2041 caaaggaacc agcttcagaa ttttcaatag ctaataactc ttccttagtc atacaataaa
     2101 tacccgctgc attcatcaaa caattgtcaa aactaaaacc agcaatttcc gtttttagcg
     2161 ataccataat atcctcctga agataaattt ttactatttt atcacttgat taatagagct
     2221 gcaagaaaat aatgataatg gtaataaaat ttcgctaaat aacgaacatt attgtttgta
     2281 taaacaataa tagtaaagaa ataaatgatg ctttctataa tgcataaaaa ataagcttat
     2341 gtaagcttat tttttatgtt cggcgtaaga ttttctttag taattgaata ctcttatatt
     2401 ttaatggatt tggatagtag cggaaagtac ccattttacg aacaatgtaa ccattaaaat
     2461 tctgtttaaa gcgaagtacg ccatctgaac catcaaaatt tccttgaata cctaaaaaat
     2521 tatagaaagt tgattgacgc tttaaagcct cttgcataac atattcttga agaactgcag
     2581 gcgcataaaa tttattaaat tctgtgtatg agccagagaa aagatagaca gtttctttcg
     2641 ggctataaat aaagaggcta ccagctaaaa caacatcttg atttccaaat ttttgaataa
     2701 aaggttcgac ttctttgcga cgtttatcta aactagctag ttgattgaca agctcagtac
     2761 gttgacgatg gtatttggca gaatctgtat tattttgatg gcgattatca aggactgtaa
     2821 tctgttcttc caatttcttt gcatttaatt gtaggttatg atcatattcc ctaaagttta
     2881 gagttgcaat gacaaattcc gccttatctc cgaaactatc ataaaaatct tgataataat
     2941 ctagagattt atccatatag tcccggcgat cagaagtaga agaagtaata tccttaaaga
     3001 tatgtagttc ttcccgcttg aggacacgta tttttatacc aaaagacatg gcttttttaa
     3061 ccaaggggcg tcctttttta gagaaagatt taagaagatt ttgaggagtg ataccttcta
     3121 gatttttaac ataatgccaa tcaggttcac cgccaggata accaatatga agcccatcgt
     3181 ggtgataacc tatactagtc aggtcatcaa taagataggt gttcggtgca cctttaggct
     3241 taccttcacc agtaaactct tggtaggtat cataaggttt aattaataat tctaaagcgc
     3301 cctgagattt ggcatagttc tgtagttctt tataaaaatg tttcagatat ttagaattgg
     3361 aatgggctgg accagaattg acttccatat gaagtccacc agtcattggg atggtataaa
     3421 caatactgat gatttccatt ttcccatcga tttggtagcc catgtaggta atgtcatacc
     3481 cacgtttttt tagaagcttt gccatctcag gggtttgcat aaacgattgt aaatcgtaat
     3541 ttcctgaata actttcgaac tctttagctg ttaattcttt aagcgccata aaagtatcct
     3601 ttcctaacta gctattacgt tttttgcgta aggcatagac ataattagag gctttataaa
     3661 gcaatctgtt cactggaatg ttaaattctc caataaattc ctcaatgatt ggttcgaact
     3721 tagacttaaa atgatataaa ccaccagata agtcattctc aatccccccc atattttgcc
     3781 aaatatttcc acgttcaaaa gccctttgag ctgtttcaaa ccaagtgtaa ataggagcag
     3841 aataagattt aaagtaatcg tccataccag cataaatatt ttctgatgta ttaccaaatt
     3901 ctagagtgag ggttgcagcc aatggaataa tgtctcggtc ataggctttt tgagcttctt
     3961 tgagaaaatc aatttcttta tttaatcgat caatcgtacc ttggtgttgt ttaacttttt
     4021 tctcacgatt aagttcaagt gattttattc tttcagattg ggctatctga cattcttttt
     4081 ctattttttc taatcgtttt gcaacatcta gcgaagccat tgtgatgtat gagttattag
     4141 gatacgtatc aaggagtttt tggtagtagt caattcctct aagatttatc cctttacggt
     4201 cttccgtttt tttcatcaac tctgcaaaat cttcaagtag ttcatggcta ccaaattgta
     4261 tatctactcc cttatttttg gaagttcgaa tagcttgacg agttttttta gacattttat
     4321 caagtccaaa ttgattagca tataggtttg cctgaaaacg tggttggata gtatgagcta
     4381 aatctgttgt ccgaccagtc caatcagcac ctgcacgctg taaatgatca atggcgatta
     4441 acccttcttt ttcatcgggc gaattttcaa aatctttggc attgaccatt tttaaataga
     4501 tgagaggatc acacttgata aagagagctc tttgtttttt gccaaaagct ttaaggtcct
     4561 taaggacctt agttacaata tctagattgg agtaatccat gattggtcca cgcggaatat
     4621 atagcatgga gaagccaaga ggaagtgatt tgatgagaat actagcgctg gcaatttggg
     4681 tttcaccgtc aaaaaagcca agtaattgac taccccagtt gtcttttact ttgggccaat
     4741 tagaagattg aagtaggttt gtttggttgg attcgctcac aaagcgatcg tgttcgacag
     4801 cggtaatttc tctatacatg agtttccttt cttagtgaga gtgtctttgt ttttttcgaa
     4861 tagtataggc taggttggcc aatttatata ataatggatt gattgctaga ttaaactcgc
     4921 caatatattc ttcaaacatt ggattgaagt tacttttaaa ttttgttaaa ccatcgttaa
     4981 gacttccctc gacaccgcct agattagcag atacaatatc gtttttgaaa gcatcttcga
     5041 acaccctagc attcaagaga tactgtggat aaaatttacg gaagctatca tccataccag
     5101 catacaacat ctccaggaca ttgccaaaac gaatggacaa aattcctgca atgacaggct
     5161 catctggata tttcttgcta aactcctgaa attcaaggat atattgttta acagaagctt
     5221 cttgttgatt tagccttgtc aggcgacttt tttgatggga tggagtttct gataaatctt
     5281 tttggatttg aagaagttgt tctttaaatt gcgctaatct tttaggaaga ttgactttag
     5341 ctaaatagag ataggcatct tctccataaa ttgtcattag ttgatggaaa tagttttcat
     5401 tacgtaaagc tacaccttta cgattctcgg ttaatgctac aaccgtggcg aatttaggta
     5461 aatcatcaat gttggcacga taaatttgaa caccgcgatg ctttgcatct tttatcagac
     5521 gtttagtgtg ttttggaaag ttttcttcta tatttgcttt ggtatagata ttcgcttgaa
     5581 aacgaggttg gatggtttcg gaaatggctt tcgttggacc tatccattgg gcacctgctg
     5641 acttaagatt atcaattgcc tgtctacttt catctatctc ttcggcgaca ttctcttctt
     5701 taaggtgata ttgacgtaga agaagtgctg gatcgaattt tgcaaaaaca gcacgtttct
     5761 ttcgtccaaa gttttttagt gtttttaaca caaagttaac taattcttta ttgctgtagt
     5821 ccataatagg tccacgtgga atataaagca ttgtaaaacc aagtggtaat gatttaatta
     5881 agatgctagc tacagcaact aattggtcat ctttataaaa gcctaaacgt tcattttgcc
     5941 aatttgattt tacttgtgcc cactttgaag agtggaggag attggtttga gacgatgcaa
     6001 tagcaaaggc atcatattct ttttcggata ttccaacggt aacatgatac atcagtttat
     6061 tacccattca cgtagtgcgt aggctacgcc actttcctcg tttgatttgg tgatatattt
     6121 tgctattttt tttagttcag gattgccgtt ttgcataaca acaggatttc caacaacttc
     6181 aagcatagca cggtcatttt cttcatcacc aatagccatt gtttgatcta cagttaatcc
     6241 cattttctct gcaaggtgga gaagtgcagt gcctttatta acgtttttgg gtaaaatttc
     6301 taaataaaaa ggagttgatt ttacaatagt gtagttatcg agaacttttt ttggaatttt
     6361 agcaattgct gcatctaaga tgtcaggttg atcaatcatc attagtttaa tgatttcttt
     6421 gtctcccatt tcttcaggag tacgatagaa gagcggcata ttaactaaag ttacttcgtg
     6481 tattgtatat ttaccaatat cacgatttgc tgtgtaaatt ccttcttttg taatggcgtg
     6541 catgtggacg cctaatttac gcgctaacag ttcgaaatcc agatagtctt cataggttaa
     6601 ggtttctttg atgatgtcat ctcctgtggc tgtgtcttga actagaccgc cgttaaaggt
     6661 gataacataa tctccctctt cattgagttt caattcttct aaaagatctt ggactccgat
     6721 gattggacgt cctgtagcaa taacaatttt aacgcctttt gattttgctt cttgaacagc
     6781 tttagccact tcaggtgtaa tttcacgctt actatttaat agggtgccgt caatatctac
     6841 ggcaacaagt ttaattgaag tcattttatc tcccactctt tactcaatat cctaaaatac
     6901 tcaccttata tcttactaaa aaataaaggg tttgtcatga atggctatta tctttcgtga
     6961 gtgtcaaatg ctcatttgtg atataagatt gaaattcttc ttttgtactt gaaaataggc
     7021 tatctaatgt caacatttct tttggaaaat aaaaacgttg atcaccataa ttagatccag
     7081 ttaatgcttt tacaatcggt gataagcttg ataattcagc aagttgtcca ttcttttgga
     7141 taatttgaat ttctgttcgt ggatttgaat gttcaggccg ataaacatcg taaggtaagt
     7201 caaaattagc atggacacca gtgtaataat cgggatcaaa accaacctgt gagactagtt
     7261 ctttcatttt aactagattt tctttatcag actcttcaaa ggtaattgat ttaaagactt
     7321 ttcgattgat aaaacgatta gctaaatctg ccaaaatgtt atcatcagct tgcatccaag
     7381 attggaagta agtattcatt accccatcat ctagggctaa ataatcttgt aaatcatatt
     7441 gatccgtgaa gaaaggaata agatttggag aagtttgttc aaaaaaatcg cgatgggtat
     7501 caaataagaa gcgagcacgt tttagtaaat tttgtaagag taattccata gcacgactgg
     7561 ctggatggaa ataaacttgc atatacattt ggaagcgact aacaatataa tcttccacag
     7621 cgtgcatccc gttacgagca aaagcaattc cagaatcggt tggtctaata acgcgtaaaa
     7681 ttcgtgttaa atcaaattgt ccatagctag cagcagtgta gtaagaatct ctgagaagat
     7741 agtccattcg atcacaatca atttgactag aaattaattg aacaacttgt ttattggggt
     7801 agctatgatt aataacacta gctactttat caggaaaatc aggagccacc tttcgtaaaa
     7861 tagcattaat ctctgtggta ggattggtga tgatttcctg agtgtaagct tcatgatcag
     7921 tattgaagag acgctcaaat gtatgagaat atgcgccatg tccgatatca tgtagtaggg
     7981 cggcagccat cgttagcagg gactcatttt tattccaaag atcagaataa tgttcatcaa
     8041 agatttctgt aactttacga gccagttcat aaactccgag acaatgtgaa aaacgactat
     8101 gctctgcacc atggaaggta aaagaagtcg ttgaggtttg tttaatgcgt cgtaaacgtt
     8161 gaaattcttt agtgttaatc aagtcatata tgacttgatt atttacatgg atataagtgt
     8221 gaacggggtc acgaaatact ttttcattca tatatatatt atagcaaaaa ttgaaacttg
     8281 agtcagaaat tggtagaata gaaggataaa agaagtgaga gaagtcatga aactagaaat
     8341 taacaataac attcaaattg acaacgaaac cgaaatgatt catgagattc acgattgtca
     8401 atttattgaa aaaggaagtt acgtttattt gaattatatt aatgctgagg gcgagagggt
     8461 agttattaag gcaaatcatg aggaattgct tatgacgagg ttttcaaatc ctaagtctgt
     8521 aatgcgtttt catcgtgaaa cgcctgcttt ggttaatatc cccactccac ttggtgttca
     8581 acatttgatt acagagacaa gtcactatca gtttgatttg tcacagcaaa ggttgcatat
     8641 caattatgtc cttaagcaaa cggagacagg agactgtttt gcaaattatg aattaaggat
     8701 tcaatggtat taagataact accattaaca aaagagtagt cgttatgaat ttcgtttact
     8761 gaagacgctg ggatctcttt atgagagcca tttatttgat aaactgcagt gtggtttatt
     8821 tcgactagat gaacttcgtt ttttcccttg agattattag tgatagcaag gataattaat
     8881 tgaaaaatgg atttttttct gttacaatga ttaacatatc aagggagtag cagacggttt
     8941 accgtggcaa tgtcgtcatt acgaaagaaa tttccggcgt tgtcttaaac agcgagactt
     9001 gttttcataa aaacaggtct ctttttgttt gtggagagac caaaggagga aaggttttgt
     9061 taaaagaaca gaaaaaatcc ttgttttaca cacaaggtca agaagaggtt ctaacgtctt
     9121 tagaatcttc tcgtgaagga ttatcaacga cagaagcgaa aaatcgctta gagatgtatg
     9181 gtcgcaatga gcttgaagaa gggaaaaaac gtagtctaat agctaagttt tttgaccaat
     9241 tcaaagattt aatgattatt atcttactgg ttgctgcagc tctctctgtt attacggaag
     9301 gtatgcatgg cttaacggat gcattgatta tcttggcggt tgttatctta aatgcagctt
     9361 ttggtgttta tcaagagggg caagccgaag cagctattga agccctcaaa gatatgtcaa
     9421 gcccaattgc gcgtgttcgt cgtgatggtc atactattga ggtggattct aaagaactag
     9481 ttcctggtga cctcgttatg ttagaagcag gagatgttgt tcccgcagat ttacgacttt
     9541 tagaagccgc gtctctaaaa attgaagaag cagctctaac tggtgagtca gtacctgttg
     9601 agaaagacat atctcaagta gtagcagagg atgcaggtat tggtgaccgt gtgaatatgg
     9661 cgtatcaaaa ttcaaatgtc acgtatggtc gaggatatgg tgttgttact aacacaggga
     9721 tgtatactga agttggtaaa attgcggaca tgttggcaaa tgctgatgaa tctgaaacac
     9781 cattgaaaca aagtttagtt caactttcta aattattgac ttacttaatc gtgattattg
     9841 cggttattac tttcttagtg gggatatttg tccgtaaaga aggttggatt gaaggattga
     9901 tgacttcagt tgctttagca gttgcagcta ttccagaagg gcttcctgca attgttacga
     9961 ttgtcttatc aatgggaaca aaaactcttg ctaaacgcaa ttctatcgtt cgtaaactcc
    10021 ctgcagttga aactttaggt tctacagaaa tcattgcatc agataagact ggaactctga
    10081 caatgaatca aatgactgtc gaaaaagtgt atactaatgg tgttctccaa tcatcttcgg
    10141 aagaaatttc agttgataat aacactttac gtattatgaa tttctccaac gatactaaga
    10201 ttgatccttc aggaaaatta attggtgacc caacagagac tgcactggta caatttggtt
    10261 tagataagaa ttttgatgtc cgtgaagttt tgaaaaatga accacgtgta gctgagttac
    10321 catttgattc agatcgtaag ttaatgtcta cgattcataa agaatcagat ggtcgctact
    10381 ttatcgcagt caagggtgct cctgatcaat tattaaaacg agttactaaa attgaagata
    10441 atggcttggt tcgtgatatc acggctgaag ataaagaggc tatcctaaat accaataaag
    10501 aactcgcaaa acaagctctt cgtgtcttga tgatggctta taaatatgaa acacaaattc
    10561 caactttaga gactgatatt gttgagtcag atttagtatt ctcaggttta gtgggaatga
    10621 ttgaccctga gcgtccagaa gcggcagaag ctgttcgagt tgctaaagaa gcaggaattc
    10681 gtccgattat gattaccggg gaccatcaag atactgcaga agctattgca aagcgtctag
    10741 gtattattga tgcaaatgac acagaagatc atgtctttac gggtgctgaa cttaatgaat
    10801 taagtgatga agagtttcaa aaagtattta aacaatattc tgtctatgca cgtgtttctc
    10861 ctgaacataa agtgcgtatc gtgaaagctt ggcaaaacga tggcaaagtc gttgccatga
    10921 ctggtgatgg tgttaatgat gctccatcgc taaaaacagc agatattggt attggtatgg
    10981 gaataactgg tacggaagtt tccaaaggtg cttctgatat ggtccttgct gatgataatt
    11041 ttgcaactat tattgtggct gttgaggaag gacgtaaagt cttctcaaat atccaaaaat
    11101 ctattcagta tcttctttca gccaacatgg cagaggtatt tacaattttc tttgcaactt
    11161 tattaggatg ggatgtactt gcaccggttc accttctttg gattaacttg gtaacagata
    11221 ctcttccagc catcgctctt ggagttgagc cagctgaacc aggagttatg acacataaac
    11281 ctcgtggtcg ccagtcaaac ttctttgatg gtggtgttat gggagctatt atctatcaag
    11341 gtattttaca aactatctta gtacttggtg tctatggttg ggcattgatg tatccagaac
    11401 atgctggtta tcgcatgatt cacgcagatg cccttacgat ggcctttgca acgcttggtt
    11461 taatccaatt ggtacatgca tttaacgtta agtcagttta ccaatcaatc tttacagttg
    11521 gagcttttaa aaatagaacc tttaactggt ctattccagt agctttcatt cttttgatgg
    11581 tgactattgt tgttcctgga tttaataaac ttttccatgt aactcattta agttcaacac
    11641 aatggttaac tgtagttatc ggaagtttat tgatggtagt gttaacggaa attgttaaat
    11701 ttatccaacg taaattagga caagacgaaa aagctattta gtttagctga taaaaaaact
    11761 cacttagaat atctaagtga gttttttagc aatcaaatat tgtcatagag ttgcgtaatt
    11821 cgtcttcaaa atattttttt cgatagtttt caaaactatc caaatgctta acggcattat
    11881 accttttgct aagattccag gttaaattag ggttatgatt gggattctga ttgacaaagt
    11941 caatagttaa attccattct ctaatatatc caagtggtct agaatgataa gtagtctctc
    12001 caattttaac gtcgccaaat gagcggtgtg catgaccaaa aacaacatct ttgatatgat
    12061 atttttggaa taaatcatga taagcctgac tccccaaaaa agcattaaaa ggagagaatc
    12121 taggatgagt catcgtgaat tgtttatggg gaacaaagtg catagcaata atgatatttg
    12181 aagaatcgac ttttgctaat atttcgtcta agcgttttaa aattgatgct tggatagtta
    12241 catcattatt tggacgtttt aaacgtctat caaaccaaaa agtctttttt aatttttcaa
    12301 catccttgat gtctctatta tttgaaaagc tgtaatcata ccagccatga aaagctagta
    12361 gcattttttt gtcaaatcga taggtttgaa agtcaagacg ttggatttct gtttctgtta
    12421 aatcaagcat atcatggttt cctaaattat agctaagttt aatatgcttt tttaagttat
    12481 tgataaaggg aagtgtatct ttagtaaaat gattagatat atctccagcg atatggaggt
    12541 gatcaatttt ttctttctta agcaaattaa ttaacgtttt cgtctcaaag gtagaaaaat
    12601 gattgaggtc aatatgtaaa tcactcatga tagcaagttt ggtcataagg gtattgtacc
    12661 atattgtagt tcaaagagtt aaacagtaaa tttttgaaat agttttagag tcattctata
    12721 atagatatgt cattgtagaa tgcgctttca taagtgtgtt tagaagtttt atatttaagg
    12781 tgccttaagg aggtctcatg tctaattttt ataagctctt gaaagaaaaa tttcctcgaa
    12841 aagaagatat cgtaacagaa atgatcaatt tagaagctat ttgtcaatta cccaaaggga
    12901 cggagtattt tattagtgat ttacacgggg aatacgatgc tgttgattat ctcttacgga
    12961 caggtgcagg gtctattaga gctaaattat tagactgttt tgattggcag aaaatagttg
    13021 ctgttgattt agatgatttt tgtatactgc tttattaccc aaaggaaaaa ttagccttcg
    13081 ataaaatgaa cttgtcagca agtgcttata aaacgaagtt atgggaaatg attccgttac
    13141 aaatacaagt tttaaaatat ttttctagta aatatacaaa atctaaagta cgtaagcaat
    13201 tgtcaggtaa gtttgcttac attatcgaag aacttttagc tgagattgac cgtaacccag
    13261 agaagaaaag ctattttgat actattattg aaaaattatt tgaattagac caagtagaag
    13321 atttaattat agtattgtcc caaacaattc aagttttgat tattgaccat ttacatgttg
    13381 taggggatat ttacgatcgt ggtaggtatc ctgatcgtat cttgaatagg ttaatggctt
    13441 ttccaaattt ggatatccaa tgggggaacc atgatgtcac ttggatggga gcagcatctg
    13501 gttcttacct ttgtatggta aatgttatcc gtattgctgc acgttataat aatattactt
    13561 taattgaaga ccgatatggt atcaatttgc gtcgcttggt tgattactca cgccgttact
    13621 atgaacctct accttctttc gttcctatat tagatggaga ggaaatgact catccagatg
    13681 aactagactt gttaaatatg atacaacaag caacagcaat tttacagttt aaattggaag
    13741 cccaattgat tgacaggcgt ccagaatttc aaatgcataa tcggcaactt attaaccagg
    13801 taaattataa agatttaagt atatctatta aggaagttgt gcatcaactt aaagatttta
    13861 atagtagatg cattgattcg aaaaatccca gtcgattaac aagtgaggaa gaagagcttt
    13921 tacaacaatt gatgatagcc ttccagactt ctgaaagttt aaagaaacat attgatttcc
    13981 tatttgaaaa aggaagtatg tatttaacat ataatgataa tcttttattc catggttgca
    14041 taccaatgca tagtaatggt gattttaaaa gctttaaaat tgcaggaaaa acttatggtg
    14101 gtagggattt actggattta tttgaaagtc aaatacgtct agcatatgct agaccagaga
    14161 aacatgatga tttggcgaca gatatcattt ggtatctttg gtgtggtgaa aattcttctc
    14221 tttttggtaa gaatgccatg actacttttg agcgctatta tgtttccgac aaagtaactc
    14281 atcaagaaag aaaaaatccc tactttaaac tgagagataa agatgatatt tgcactgctc
    14341 ttttacaaga gtttgatttg cctaagtttg ggcatattgt caatggtcat acgccagtca
    14401 aagaaaaaaa tggtgagcaa ccgataaaag caaatggtaa gatgttagta attgatggtg
    14461 gctttgccaa aggttatcag aaaaatacag gtttagctgg ctacactttg atttataata
    14521 gctatgggat acaattaatt tcacaccttc cttttacatc cattgaggaa gttttaagtg
    14581 gaaccaacta tatcattgat acgaagcgat tagtagaaga agccaaggat aggattcttg
    14641 taaaagatac aactattgga caaaagttaa ccaaagagat taaagactta gatcaccttt
    14701 atcgtcattt tcaggaatat gatgactagt gtttaagtaa tttactaatt gatatgtagg
    14761 atttaatttt tcagtttctt accttgtctc ttttattaaa ttgacttaaa ataatattat
    14821 ggacataaag gcagaaattc aaaaattagc aaaagaaatt ggtatctcta aaattggttt
    14881 tacgacggca gataactttg attatttgga aaagtcattg cgtgcttctg tggaagaggg
    14941 gcgcaattca ggatttgaac ataaagtcat agaggatcgt atctaccctg aaaggctttt
    15001 agaatcggct aaaacgatta tttcaattgg cgttgcttat cctcacaaac ttcctcagca
    15061 acctcaaaaa acatcttata aacgaggaaa aattacacct aattcgtggg ggcttgatta
    15121 tcattatgtg gtcggcgaaa agttagacag actatcaaaa ggcattgaag agctctgtcg
    15181 agattttcct ttgcaacaaa aagcaatggt cgatactgga gctttagttg atactgcagt
    15241 tgcccaaaga gcaggtattg gttttatagg aaaaaatggt ctagttattt ctaaagaata
    15301 tggctcttat atgtttttgg gagaattaat taccaattta gagatcgagc cagacaagcc
    15361 tgtcgattat gattgtggtg actgtcgtcg ttgtctagat gcctgtccaa cttcttgttt
    15421 aattggtgat ggctcaatga atgccaagcg ttgtctatca tttcaaaccc aagataaggg
    15481 aatgatggac atagagtttc gaaaaaagat taaaacagtt atctatggtt gtgatatttg
    15541 ccaaatttgc tgtccttata acaaaggtat aaataatcca ttggcgacag agattgatcc
    15601 tgagttggct caaccagaat taataccatt tttgagttta tcaaatggac aatttaaaga
    15661 aaaatttggt atgattgctg gaagttggcg aggtaaaaat attttacagc gtaatgccat
    15721 tattgcgcta gcaaatgctc atgataaaac cgcagtggtc aaattaatag agattattga
    15781 taaaaataat aaccctattc atactgcaac agctatatgg gcattgggag aaattgtcaa
    15841 aaaaccaaac gatgagatac tggagtttat gagtaattta accttaaaag atgaagatag
    15901 ccgaaaagag ttggaattaa tccgtcataa atggcaattt taagcctttt atggtatttt
    15961 taagaacctt atgataaaat aaaacagatt aacaaagatt ggagaaatct catggaagtg
    16021 gctgaaattc gccaaaaaat agtagaaaat aaagagaagt tgactagctt caggaggtct
    16081 ctttgactta gatcgtttgg aagaggagat tgctcttctt gaaaatcaaa tgactgagcc
    16141 agacttttgg aatgacaata ttgcagcgca aaagacatct caagaactga atgagttaaa
    16201 aggtaagtat gatactttcc ataatatgca agaattatct gacgaaacag aactcttact
    16261 tgagatgctt gacgaagatg attctttgaa agaagaatta gaagaaaatc taatgcagct
    16321 agataagata atgggtgctt atgaaatgac gcttctctta tctgagcctt atgaccacaa
    16381 taatgctatc ttagaaatcc atcctggttc aggaggaact gaagcgcaag attggggaga
    16441 tttactctta cgaatgtata cccgttttgg caatgctaat ggtttcaaag tagaagtatt
    16501 agattatcaa gcaggggatg aagctggtat taaatcggtt accctctctt ttgaaggccc
    16561 caatgcttat ggtttgttga aatcagaaat gggagttcat cgcctcgttc gtatatcacc
    16621 ttttgattcg gctaaacgtc gtcatacctc atttgcttca gttgaagtga tgccagagtt
    16681 agatgatacg attgaagtag aagtacgtga tgatgacatt aaaatggata ctttccgttc
    16741 cggaggagct ggtggtcaga acgtcaataa agtatcaaca ggtgttcgtt tgacgcatat
    16801 tccaactgga attgtggtat cgtctaccgt tgaccgtact caatatggga accgcgatcg
    16861 cgctatgaag atgttgcagg ctaagctata tcagttagaa caagaaaaga aagcacaaga
    16921 agtagatgct cttaagggtg ataaaaaaga aatcacttgg ggaagtcaga ttcgttctta
    16981 tgttttcacc ccttatacga tggtgaagga tcatcgtact aactttgagc ttgctcaggt
    17041 agataaggtt atggatggag aaatcaatgg ctttattgat gcctacctta agtggcgcat
    17101 tgaagactaa aaataatgaa ggagaaacat ggcactaata gaaatgtctg gtgttactaa
    17161 aaagtatcgc cgttcaacaa ctgccttgag aaacttaaat ttatcaattc agcaaggtga
    17221 gtttgtgtat ttagttggtc cgtcaggtgc tggtaaatct agcttgattc gcctcctata
    17281 ccgtgaagaa aaattaagtt caggtagatt aaaggtaggg gaattcaatc tcaataaact
    17341 caaacgtcgt caaattccta ttttacgtcg tagtattgga gttgtcttcc aagattataa
    17401 attactacca acaaaaacag tttatgagaa tgttgcgttt gcgatgcaag ttattggagc
    17461 taagcgccgt catattaaaa aacgcgtgcc agaagttcta gaattagtag gtttaaaaca
    17521 caagatgcgt tcttttccaa cacagttatc tggtggagaa caacaacgtg tggcaattgc
    17581 acgtgcgatt gttaataatc caaaattatt aattgcagat gaacctactg gtaatttgga
    17641 tccagaaatt gcttgggaaa tcatgcatct attggaacgt attaaccttc aaggaacgac
    17701 tgttttgatg gcaacacaca atagtcaaat tgttaacaca cttcgtcatc gtgtcattga
    17761 gattgaagca ggtagcgtta tccgagatga agagaaggga gaatatggtt atcatgatta
    17821 atttctttag acatttctgg gaatcgctta aaaacttgaa acgtaacttt tggatgactt
    17881 ttgcttctgt aacatcagtt accataacct tacttcttgt aggcttgttt tcatcagttt
    17941 tactgaatgt tgaaaagtta acaactgatg tttcaggtaa ttttactatc agtgcctttt
    18001 taaatgttga ctcaacggat gctcaaaaac aagtcaaaga taaggacgga aagctaaaag
    18061 ataatcctga ttatcataaa gtttacgaca aaattaaacg tatttctggt gtagagaaag
    18121 tcacttattc aagtaaagct gaacaattaa aagaagttca aaaagagtat ggttcagatg
    18181 ttattgatga tacctataaa gatgctttgt tagatgttta cgtagttggg acaagttctg
    18241 caaaagtttc taagagtgtc agtgaagcaa ttggacgcat tgaaggtgtt gattatacta
    18301 aagaacctat tgattcaaca aaactatcta atctcacaga taacatccgt atatggggat
    18361 ttggtggtgt tgccttatta attgtccttg ctatcttttt gatttcaaat actattcgta
    18421 tgtcgattat gtcacgccgt acagatattg aaatcatgag attagttggc gctaaaaatt
    18481 cttatattcg aggaccgttc ttttttgaag gtgcttgggt agggatttta ggggctatcg
    18541 ttccatcgct gattttctat tttggttatc aatttgtgtt taacaagttt aatccaaaat
    18601 ttgagacaag ccatgtttca ttgtatccta tggatattat ggttccagca attattggtg
    18661 gtatggtgat tattggtatt attattggct cacttgggtc tgtcctatca atgagacgtt
    18721 accttaagat ttaataaaaa gagatgaaat ctatttcatc tctttttatt aattgtagat
    18781 atcatgttag tggataaaat tcgaaataaa cgtagccaat tgtttttcag atgatttgtg
    18841 agaaagggta gctttaccat cccctttttg atgtccgtag ttaccaaatc ggctatggtt
    18901 tcctccgaca atagttcgaa aagtacttga atttgggagg cgttttttag cttcctcata
    18961 tttttcccag tttaaaatat gatcattact tgctgtaatg gagagtactc ttaacttttt
    19021 atgagatagg tcggatttac gtgaagggta ggaagctaat aatatgagcc ctctaacagg
    19081 ggctactttg gcattttggc tagcaacaac tcctcctaaa gaatgtccag caaggtaaac
    19141 attagttaag tggttctgat taataattgt tttagcttta tgaggcgata aaactggcaa
    19201 atttaaaggt gtcttcaaaa tataggtatt atcccctttg tcagctaaat ctctagcaag
    19261 ttgagaataa gcttcttctt ccacaagagc cccttgatag aaaataatat ttgcttttac
    19321 cttacccttg cttctaaaaa gaaggtagtt cttatggttt gtagcggttt tagcaattct
    19381 cctagctaat ggggaggctt ggtaagtatt gaagtaaatg gtcatcccga cggccattaa
    19441 aatagcaagg atacttgttg ttaaccaaag aaaaatagag tgacgtttta tgttcattgg
    19501 aagaaaggat taaagttttt ttcgtggcca atagttgtat tcatgccatg gcctgggtga
    19561 acactataat gactaggtaa ggtgaatagt tcttgacgaa taccagtaat gaggtcctca
    19621 aagttactgg taggtaagtc agtacgaccg atagtttcac gaaatagagc atcgccagta
    19681 acgactaatt catctgagtg gaaaacaaac gagactcctc cccaagaatg accaggtgtt
    19741 ggtaaaactg tgaattcaaa accatttagt tgatagggtt gctttaattt aaaaaagttc
    19801 tctgccggtc ttgctataac attaatgata tcatcatgac ggcctaaccc agataaatta
    19861 tcatctgggg aagatagcca agcggcttct ttttctgaaa cataaacagg tggattatca
    19921 aaagtatctc tgaccaaatc taaactaaaa atatgatcat agtgagtatg agttaagagg
    19981 atagccacta aaggtttttc aaaggattta attttagcaa taatttcttg gccattgctc
    20041 cctggatcaa tgaggataac agcttgatca ttaacgaggt aataagtatt ttcaccagca
    20101 atgtggttta ttgttttata gattttcata atgatagtgt accaaaaaat caagactttg
    20161 ttaaaaaaag agtggcggaa aataaatggc aactagtttc agaaaatatg ggatctcaca
    20221 gcaatttctt gacaccgttt tcataaaggt gtatcataaa agtaatcaat aagccaagcc
    20281 taaagctgaa cttagtttaa gaacactgac tttttgagag caacctaatt ggttgactaa
    20341 agaatatgag gtggaagata tgtcaaaagt tgctattgtt actggtgctg gtcaaggaat
    20401 tggatttgct attgcgaaac gtcttcatgc agacggcttt aaaataggcg ttttagacta
    20461 taatgaagag actgctcagg ctgcggttga taaactatca cctgaggatg ctgttgcagt
    20521 tgtagcagat gtttcaaaac gtgatcaagt ttttgatgct ttccaaaaag ttgtagatac
    20581 ttttggtgat ttgaatgttg ttgttaacaa tgcaggtgtt gccccaacaa ctcctcttga
    20641 cactattact gaagagcagt ttgaaaaggc ttttgcaatc aatgtcggag gaactatttg
    20701 gggaagccaa gcagctcaga aacattttag agaattaggt catggcggaa aaattataaa
    20761 tgcaacttca caagcaggat gtgaagggaa tcctaatttg actgtttatg gtggtacaaa
    20821 atttgcagtt cgtggtatta cacagacttt ggcaaaagat ttagcatcag aaggaatcac
    20881 agtcaatgct tacgcaccag gtattgttaa aacacctatg atgtttgata ttgcccatga
    20941 agttggtaag aatgcaggca aagatgatga atggggtatg gagcagtttg ccaaagatat
    21001 cactttgaaa cgcttgtctg aaccagaaga tgttgctaat gcagtaggct tcttagcggg
    21061 agatgattca aattatatca ctggacaaac aatagttgtc gatgggggta tggtattcca
    21121 ttaaaaatga ttaatgctaa cactgtgagt ttcactcaca gtgtttttta tatttcttaa
    21181 aaaattatct tatcttggtt attggagtga tggcatcttt gaggtatagt catgttttgt
    21241 tttatagata tagcgtgcta taatagactt actatgaccc agaaaaaact tagaaaatat
    21301 gcagtagttg acttggaggc tactggagca ggtcccaatg cttctattat tcaggttggg
    21361 attgttatta tccaaggaaa taaaattatt gatagttatg aaacagatgt taatcctcat
    21421 gaatctttgg atgagcatat tgttcatttg acaggtatta cggataagca gttggctaaa
    21481 gctccggatt ttggtcaagt ggcgcatcac atttatcaat tgattgaaga ttgtattttt
    21541 gttgcccaca atgtaaagtt cgatgcaaat ttattggctg agcaactatt tttagaaggc
    21601 tgcgagttac gtacaccaag gatagataca gtagaattat cacaagtatt ttatccctgt
    21661 ttagaaaaat attctttagg ggcattagca gaaagcttaa atattgaatt aacagatgcg
    21721 cacactgcta ttgcagatgc aagagctaca gcacagttat tcattaagtt aaaagctaaa
    21781 attagtagtt tacctaaaga agtgttggag accattttaa catttgctga taatcttttg
    21841 ttcgaatcct atttattgat tgaagaagct tatcaagaag ctgatttcgt taatcctaaa
    21901 gaatattatt tctggcaagg attggtctta aaaaaagaaa aagctgttgg aaagcctaag
    21961 aaattatcta gtgattttca agttaacatg gctttgttag gtatggatgc tcgccctaag
    22021 caagttgttt ttgctgactt ggttaaagcg cattttaatg atcaaacaac aacgttttta
    22081 gaagctcaac caggacttgg taaaacatat ggttatctct tgcctttatt ggatcagtct
    22141 caaaaacaac aaataatagt atctgttcca acaaagattt tgcaagatca aataatggct
    22201 aaagaaataa agcacattca agaacttttt catattccct gtcacagtat caaaggaccg
    22261 agaaattact tgaagttaga tgcgttttat aagagcctcc aagtgcagga tcgaaatcga
    22321 ttgataaatc gttttaaaat gcaactgcta gtgtggttga cggaaacgac tacaggtgat
    22381 ttggatgaaa ttaaacaaaa acaacgatta gaatcttact ttgaccagct aaaacacgat
    22441 ggagaagtga ctcaatcatc acttttttat gatctggatt tttggaaacg cagttatgat
    22501 aaagttgcac aaagtcaact tgttattatc aatcatgctt attttcttga gcgtgttcag
    22561 gatgacaaag attttgcaaa ggggaaggtt ttagtttttg atgaagctca gaaacttgtt
    22621 ttgggactgg aaaatttttc tcgtgggcaa cttgatatta gtcaccaatt gcaggttatt
    22681 caaaaaataa ttgatagttc tattccatta ttacaaaagc gccttctgga aagtatctct
    22741 tatgagctaa gtcatgcagt agaacttttt tatcgtcata attcttttga attttcagag
    22801 acctggttga aacgtctaaa aaatagtata aacgctttag aagtagtagg attagatgag
    22861 ttacaaactt ttttcacagc tacttatact aactattggt ttgaaactga caaagtcaat
    22921 gagaagcgtt taaccatttt acgtggggcg agagaagatt ttcttaaatt ttcaaagttc
    22981 ctccccccca caaaaaagac ctatatgata tcagcgactt tacaaatcag tcccaaagtc
    23041 tacttgtcag atcttttagg aggattttca agtataagta cagaaaagat agcgcatgag
    23101 aaaaatgcca atcagaaagt atggattgat acctctatgc ccaatatact tgatttatca
    23161 ccggaacagt atgcttacga aatagccaag cgtttacaag atataatgac gttaaagcag
    23221 cctaccttag tcttattaac ttctaaacag acaatgttta tggtatctga ttatttggat
    23281 aagtgggaga taaaacatct tactcaagat aaaaatggct tagcttataa cgttaaaaaa
    23341 cgttttgatc gtggagaaag caatcttctt ttaggtacag gatctttttg ggaaggtgtt
    23401 gactttgtac atcgtgatcg tcttattgaa gttatcacgc gattgccttt tgatactccc
    23461 aaagactatt ttatccagaa actatcgcag agtttaacaa aagaggggaa aaacttcttt
    23521 tatgactatt ctttaccaat gactgtctta aaattaaaac aggccttggg acgtactaca
    23581 aggagagaag aacaaaagtc agcggtaatt attttggata gcagattggt tattaaatct
    23641 tatggacaaa ccatcatgca ttctcttggt agagattttg aaatttctaa ggaaaaaatt
    23701 aacaaggtct tgactgaaat ggctaaattt ttgatataat ggtaaagatc attgtctaaa
    23761 aataagattt tgacagtgcg actcaagatt aaataatagt agtacgttga taaaaggagg
    23821 agtaatgacc tatttgtcag aacgtgtact caatatggaa gagagtgtta ctttagctgc
    23881 gggagctaaa gcaagagaac tcagagtaca aggaagagat attcttagtt taactttagg
    23941 ggagcctgac tttgctactc ctaaaaacat tcagcaagcg gctattgagg ctataacaga
    24001 tggacgtgca agtttttaca caccatcaag tggtttgccg gagttgaagt cagcaattaa
    24061 tgcgtatttt gaacgttttt atggctactc ccttaaacct aatcaggtgg ttgtgggaac
    24121 tggtgctaag tttatccttt atactttctt tatgacagta ctgaatcccg gtgatgaagt
    24181 aattattccg actccttatt gggtatctta tgctgatcag attaagatgg ctgaaggtaa
    24241 gccagttttt gtcacagcaa aagaagttaa tcacttcaaa gtaactgtgg aacagttaga
    24301 agctgttagg actgataaga caaaagtcat acttcttaat agtccatcaa atcctacagg
    24361 tatgatttat aaagctgagg agttggaggc tattggaaat tgggcagtcg aacatgatat
    24421 cctaatttta gcagatgata tttacggtcg attggtttac aatggcaata tttttacccc
    24481 tatttcaagc ctttcagagt ctattcgaaa ccaaacgata gtcataaatg gagtttctaa
    24541 gacttatgcg atgacaggtt ggcgtgttgg ttttgcagtg ggtaatcatg atattatagc
    24601 ggcaatgtct aaagtagtca gtcaaacaac ttctaacctt acggctgtct cacagtatgc
    24661 tactattgag gctttaaatg gttctcaaga gtcatttgaa aaaatgaggc tagcatttga
    24721 agaacgcctt aatatcattt atccattgtt gtgtcaggtc cctgggtttg aggttgttaa
    24781 accacaaggt gctttttatc ttttcccaaa tgttacaaaa gcaatggaaa tgaaaggata
    24841 tacggatgtt acagcattca cggatgccat tcttgaagaa gttggacttg ctttagtaac
    24901 gggtgctggt tttggagcgc cggaaaatgt tcgtctgagt tatgctacag atcttgaaac
    24961 ccttaaagaa gctgttcgta gactgcatgt ctttatggga agtaatgaaa ttaattaaat
    25021 aaaaatagag agaaacttat gtcaaaaaaa cttatttcaa ttgttgatgt taaagattat
    25081 gttggtcaag aagttactat tggtgcttgg gttgctaaca aatcaggtaa aggtaagatt
    25141 gcgtttgtac agttgcgcga tggttctgcc tttttccaag gtgtcgcttt taaaccaaac
    25201 ttcattgaaa aatatggtga agaatcaggt cttgaaaaat ttgacgttat taaacgtctc
    25261 aaccaagaaa catctgttta tgtaacaggg attgttaaag aggatgaacg ttcaaaattt
    25321 ggctatgagc ttgatattac tgaccttgaa gtcattggag agtcacatga atatcctata
    25381 actcctaaag agcatgggac agacttcttg atggacaacc gtcatctttg gttgcgttct
    25441 cgtaaacaaa tggctgttat gcaaattcgt aacgctatta tttattcaac ttatgaattc
    25501 tttgaccaaa atggttttat caaatttgat agcccaattt tatcagaaaa tgcagcagaa
    25561 gattcaacag agttatttga aacagactat tttgggaaac cagcatttct tagccaatct
    25621 ggtcaattat accttgaagc tggagcaatg gctctaggcc gagttttcga ctttggtcca
    25681 gtctttcgtg ctgaaaaatc taaaacacgt cgccatttaa cagaattttg gatgatggat
    25741 gctgagtatt ctttcttgtc acatgaggaa tcacttgacc tccaagaagc ttatgttaaa
    25801 gcattgatcc aaggcgttct agatcgtgcg cctcaagcac ttgatatttt agaacgtgat
    25861 gttgaggctc tcaaacgtta tatcgcagag ccattcaagc gtgtttctta tgatgatgct
    25921 attacacttc ttcaagagca tgaagcagat gaagatacag attatgaaca tttagagcat
    25981 ggtgatgact ttggttcacc acatgaaact tggatttcta attactttgg agtgccgaca
    26041 tttgtagtga attacccagc aagctttaaa gcattttata tgaaaccagt tcctggaaat
    26101 ccagaacgcg ttctttgtgc tgatcttcta gctccagaag gttatggaga gatcattggc
    26161 ggctcaatgc gtgaagacga ctacgatgct cttgtggcta aaatggatga actaggtatg
    26221 gataaatcag aatacgactt ttaccttgac cttcgtaaat acggctctgt tcctcatggc
    26281 ggttttggta taggtattga acgtatggta acttttgttg ctggtaccaa acatattcgc
    26341 gaagctattc cattcccacg tatgttacat cgtatcaaac cataataaaa aagcgctagc
    26401 tagtgcgctt ttttattatg gtctttagaa tcgttggtta attaaattgg gactatgttt
    26461 ttttaacaat gtgattgtcg aaaagacaag gataagactg attagtcgat ccatataatc
    26521 tgttccaact tgaacaagta tcaaactact tgtcatgtct aatccaattc catgtaagaa
    26581 ttgtgctata atacttgagc cactagaggt aattccttta aaaaggataa ctgttataac
    26641 tgaagcaata atagttcctg gtaaagaaat aatcagtgat ttccacaata ggctagaggg
    26701 tttacaattc cttttaatca agatacccgt aatgatagca accacgatgg cgactggtga
    26761 ataataaagt gaaaaaatat cagtagtcat ccaattgatg agtgcactga gaaaagcagt
    26821 gctggcagcg taccatggtc ccaggataac tgctattaac aatgtaccta tagtatctag
    26881 atagataggt aggcgtaaaa atagagctaa attagcccca acaaggttga tggcgatagc
    26941 gattgagata aatgaaatta aacgagcagg tgattttgtc atttgttaag tcctaacatt
    27001 tctaagtctt caatgatgac tgaattttgt gcatgtaata ggacggtcat aaaatcattc
    27061 caaaataaat tagtattgac tgaggttaaa atcttactgt tagcatcttt tttataaaaa
    27121 tgatactgat ctacaattgt ttgtcccatg gcgattccgt gacaagcaac atcggtatag
    27181 ctatcaaaac cagtagcgat attttcatta acaaagtaag caatagctaa aggatcattt
    27241 atcacacatc caataatatg ctcatattgc cagtgaaaat cgaagtagaa ctttgtgatt
    27301 ttttggataa aacttgatac gtctggatta atacgctcca tataagataa atgattgggg
    27361 gttaatacaa tatgacgtgt gatgtctagt ccaaccatct ctattttttt gtcaaggttt
    27421 tcaaatacat attgtgcagc atgaggatca caccaatagt tgtattcagc aactggagaa
    27481 caattgccat gtgatttaaa acttcctccc atgctaataa atcgcttgca atgtttgcct
    27541 aatttgggat tagtttgtaa ggctcgagcg atattggtca gaggtccgag agctataata
    27601 gaagtatcat ttttgtgttc aaaatagttt gctaaaaaac aatctgctga ttcttcttga
    27661 aagataattg gttgcgccaa tgtaaagtta ttctctccaa ggccatccat tccatgagta
    27721 tcctgagcag atacaaattc acgttgtaaa ggcaagttat caccaacata aactggaatg
    27781 tcgtgacggt ttagtaattc aagcgttaca aaggtattct ttagtcctag ctcaacaggc
    27841 gaattgcctg ctgttatagt aatagcaaca acttctaact taggatgctg aatagcatac
    27901 atcagtgcta aggtatcatc aataccggga tcgcaatcga taataatttt ttctttattc
    27961 atttgtctcc tttcttttaa tattagtgaa tgttctatgc ttttatgaaa ctattaacaa
    28021 tagtcatggc aataaaaaag atcttggcag ttaccaagat cacacgaaaa aatgaattta
    28081 gttttctaaa ttcaatcata ttttcagtca cttagttttt tattctggga agttttcgaa
    28141 ccagtccgag aaatactcga ttatgcacgt gttttattat aatatgaaag cactaacttg
    28201 taaagattca aactgaccat ttaacagtgt ctagattgat tgttttagca tttgaaatta
    28261 attttgaaat cggacatctg ctgtgtgctt tgttaacgat tagcttacta tcagataatg
    28321 acagtttttc aatactagca acagcgttaa cttggaaata aaatccctta cctacttgct
    28381 tttccttcat gagctggcaa gtaacatcaa cgcgagattt taggtctttg aatccttttt
    28441 gttctaaaat agcttttata gttgcattta aacatgttga ccatgctaaa gcaattaatt
    28501 cctcaggatt aaatcctggt aaatgattta aagggtgatt ggtctccaca atttttccat
    28561 tggagagata aacttttcca tcaacgcctt gagtgtttga tgaggttact tcataaagca
    28621 taataaactc ctttgggtct agtttaccaa atttgggagc attccgacaa gattttacta
    28681 tttagaatga gtttcttttc ctctgaagta aaatcctaat tgctagccat ttgtggtata
    28741 atggcataaa acataagaga aagaggtaat cgtaatgtca gacgaacaaa tcaaacttgt
    28801 gattgtaaca ggtatgagtg gggcaggaaa gacagtggct attcagtcat ttgaagattt
    28861 aggatatttt acaatagata atatgccgcc tactctagtg cctaagtttt tagaattagc
    28921 agcacaatca ggagatacaa gcaagattgc tatggtagtt gacatgcgta gtcgtctttt
    28981 ctttagagaa attaactcta ttttggatag ccttgaaatt aatgataata ttaactttaa
    29041 gattctattt ttagatgcta ctgacaccga gctagtgtca cgatataaag agacacgtcg
    29101 tagccaccct cttgctgccg acggcagagt gttagatggt atttctctag agagagaact
    29161 attagcaccg cttaaaagta tgagtcagaa cgttgtggac actagtgaat taacaccaag
    29221 acaactacgt aaggtcattt ctaaagaatt ctcaaatcaa gatagtcaaa gttcttttcg
    29281 tatcgaagtt atgagttttg gttttaaata tggtatacct ttggatgcgg atttagtatt
    29341 cgatgtgcgt tttttaccaa atccatacta taaaccagaa ttaagagata agacaggttt
    29401 agatactgag gtttatgact atgtcatgag ttttgatgaa tcagatgatt tttatgacca
    29461 tcttcttgcc ttaataaagc caatcttacc aggctatcaa aatgaaggga aatctgtttt
    29521 gactgtggca atcggatgta caggtgggca acatcgtagc accgcttttg ctcatagatt
    29581 atcagaagat ttaaaagcag attggacagt taatgaaagc catcgtgata aaaataagcg
    29641 taaggagacg gtaaatcgtt catgagaaaa cctaaaataa ccgttatcgg cggaggaacg
    29701 ggaattccag ttattttgaa aagtcttcgc ttagaagatg ttgagattac agcggttgtc
    29761 acggttgcag atgatggtgg ttcgtcaggt gagttgcgct cagttatgca actaacacca
    29821 ccaggagatc taagaaatgt tttggttgcc ttgagtgata tgccgaagtt ttacgaacaa
    29881 attttccaat atcgttttgc agaaggtgat ggggattttg caggtcatcc tttaggaaat
    29941 cttattattg ctggtgttgc tgaaatgcaa ggttcaactt ataacgctat gcagagttta
    30001 acacagttct ttcatacaac aggaaagata tatccatcta gtgaacatcc gctcactctg
    30061 catgctgttt ttaaagatgg ccatgaagtg gttggagaga gtcaaatagc cgattataaa
    30121 ggcatgattg accatgtgta tgtcactaat acttataatg aagagactcc tacggccagc
    30181 cgcaaagttg ttgatgcaat tttagaaagt gatatgattg tgttggggcc agggtcactt
    30241 ttcacctcta ttttacctaa cctagttatt ccagaaatta aacaagctct tttagaaact
    30301 cgggcagagg tagcttatgt atgcaatatt atgacacaac gtggtgaaac tgagcatttt
    30361 acagacgcag accacgttga agtattgaaa cgtcatttag gacaagatgc tatcgataca
    30421 gttttggtta atattgaaaa agtgccagag tcttatatgg aaaacaatca ttttgatgag
    30481 tatttggtac aggttgaaca tgatttttca gggcttagaa aacatgccag acgtgtcatc
    30541 tcgtctaact ttttaaaatt agaaaaaggt ggagccttcc atcatggtga cttcgttgtt
    30601 gaggaactaa tgaatttagt aggaacacat ttatgagttt tacagtaaaa gtcaaagaag
    30661 agctgctagg tcataagagt gaaaataaga tggaactgtc agctattatc aaaatgtcag
    30721 gtagtttagg attagctaac cacggcctta acctttctat tactacagaa aatgctaaaa
    30781 ttgctcgtca tatttatagc atgctagaag aacattatca tttgcagcca gaaatcaaat
    30841 accatcaaaa aacgaactta cgaaagaatc gtgtgtatac tgtttttatt gaagaaaaag
    30901 ttgatgttat tcttgctgat ttgaagttgg ctgatgcttt ttttggtatt gaaactggta
    30961 ttgaacactc tatattggac aatgatgaaa atggaagagc ttatctcagg ggagcattct
    31021 tatcaacggg tactgttaga gaacctgatt ctgggaaata tcaactagaa attttttctg
    31081 tttatctaga ccatgcacaa gacttagcta atcttatgaa aaaatttatg ttagatgcaa
    31141 aggttattga acataaacat ggagcagtaa cttatttgca aaaagcagaa gatattatgg
    31201 atttcttgat agtcattgat gccatggaag ctagagatgc ttttgaagag attaaaatga
    31261 tacgtgagac acgtaatgat attaatcgtg ctaacaacgt tgaaacagct aatattgcta
    31321 gaacaatcac agctagtatg aagactatca ataatattat caagattatg gatactattg
    31381 gttttgatgc actgcctagt gatttaagac aagtagctca ggtgcgtgtg gctcatccag
    31441 attactctat tcaacaaata gcagacagtt tagaaacgcc tctgagtaag agtggcgtta
    31501 accatcgctt acggaaaata aataaaattg cagatgaatt atagaaggag atattcacat
    31561 ggcttgtaca acaatattgg ttggtaaaaa ggcttcttat gatggttcga ctatgatcgc
    31621 tagaacggaa gactctgtta atggcgattt cacacccaaa aaattaaagg taatgacatc
    31681 taaagatcaa ccgcgtcatt acaaatcagt tttatcaaat tttgaagtag atttaccaga
    31741 taacccactt ccttatactt cagtaccgga cgcattggga aaagatggta tatggggtga
    31801 agccggtatt aacagtaaaa atgtagcgat gagtgctaca gaaactatta caacgaattc
    31861 ccgcgttttg ggtgcagatc ctttggtttc agatggtata ggggaagagg atatactcac
    31921 tttagtgctt ccctatattc agtcagcgcg agaaggtgtg gagcgtttag gtgctatttt
    31981 ggaaaaatat ggaacctatg aatcaaatgg tattgctttt tcagataccg aagaaatatg
    32041 gtggttagaa acaattggtg ggcatcattg gattgctcgt cgcgtacctg atgatgttta
    32101 tgttactaat cctaaccaac taggaattga tcattttgaa tttaataact gtgatgacta
    32161 catgtgctct agtgatttga aagagtttat cgaacaatac catttagatt tgacctattc
    32221 taatgagcat ttcaatcctc gatatgcttt tggtagccaa cgtgataaag atcgtcatta
    32281 caacacacca agaagttggg caatgcagcg ttttttaaat cctgaaattg aacaggatcc
    32341 acgtagcttg tttattccct ggtgtcaaaa gccttaccga aaaattactg ttgaggatat
    32401 taaatatgtg ttgagtgatc attatcaaga cagtgtgtat gacccatatg gaccagaagg
    32461 ggatgcggta agtaggagag cttttcgttc agttggtatc aaccgaacta gtcaaacgtc
    32521 tattctacaa ttacgaccaa ataaatcact tgaaacgaca ggtgttcaat ggttatctta
    32581 tggctctatg ccatttgcaa ccatggtgcc gttgtttaca caagttgaga ctgtaccaaa
    32641 ctatttttcg aatacaacca aggatgcttc aacagataat ttttattgga ccaatcgttt
    32701 aattgcagct ctagcagatc cacactttta tcaacatgaa gctgatattg aaagctatat
    32761 cgagagaacg atggctcaag gacatgcaca tattaacggt gttgatagag aagttgctga
    32821 gaataaagag attgattttc aacagaaaaa tcaagaaatg agtgactata tccaaaaaga
    32881 aagccaagaa ttgttaaatc gtattctatt tgatgcaagt aatttaatga caaatcgctt
    32941 ttcaatggga gattaaaaaa ctcggaatat tttccgggtt ttttaagata aaaacgcttg
    33001 cataagtata taaaacttgc aaaaaaaaaa tatcgtgcta tactttacta gtaaaatatt
    33061 aaccggtaaa taacgggtta aataaaaagg agaaaaaatg agaaagaaat ttcttttatt
    33121 gatgagcttt gtagctatgt ttgcagcttg gcaacttgtt caagttaaac aagtttgggc
    33181 tgatagtaaa cttaaagtgg taacaacttt ttacccagtt tatgagttta caaaaaatgt
    33241 cgttggtgat aaagctgatg tatctatgtt aattaaagca ggtacagaac cgcatgattt
    33301 tgaaccatca actaaaaaca tcgctgccat ccaagattca aatgcttttg tttacatgga
    33361 tgataacatg gaaacttggg ctccaaaagt agctaagtca gttaaatcca aaaaagtaac
    33421 aactattaaa ggtactggcg atatgttact tactaaaggc gtcgaagaag aaggtgaaga
    33481 acatgaagga catggtcatg aagggcatca tcatgaactt gacccacacg tatggttgtc
    33541 tccagaacgt gcgatttctg ttgtagaaaa catccgtaat aaatttgtca aagcttatcc
    33601 aaaagatgca gcttcattta acaaaaatgc agatgcttac attgcaaaat taaaagagct
    33661 tgacaaagaa tacaaaaatg gtttgtcaaa tgctaaacaa aagagttttg tgactcaaca
    33721 cgcagcgttt ggttacatgg cgcttgatta cggtttaaat caagttccaa ttgctggtct
    33781 tactccagat gcagaacctt catcaaaacg tttaggcgaa ttagctaaat acatcaagaa
    33841 atataacatc aactacattt attttgaaga aaatgcttca aataaagttg ctaaaacttt
    33901 agcagatgaa gttggcgtga aaacagctgt gcttagtcca cttgaaggac tttctaaaaa
    33961 agaaatggca gctggcgaag attacttctc agttatgaga cgtaatttga aagttcttaa
    34021 aaagacaaca gatgttgcag gtaaagaagt agctcctgaa gaagataaaa ctaaaacagt
    34081 tgaaacaggt tactttaaaa ctaaagatgt taaagaccgt aaattgacag attactctgg
    34141 taattggcaa tcagtatatc ctcttcttca agatgggaca cttgatccag tttgggatta
    34201 caaagctaaa tctaaaaaag atatgactgc tgcagagtac aaaaaatatt atacagcagg
    34261 ttacaagact gacgtagaat caatcaagat tgatggtaaa aaacatcaaa tgacctttgt
    34321 acgtaatggt aaatcacaaa catttacata caaatatgca ggttacaaaa tcttaactta
    34381 taaaaaaggt aatcgtggag tacgttatct ctttgaagct aaagaaaaag atgctggtca
    34441 attcaaatat atccaattta gtgaccatgg tattaaaccg aataaagctg aacacttcca
    34501 tatcttctgg ggttcagaaa gccaagaaaa attatttgag gaaatggaaa actggccaac
    34561 atacttccca gctaaaatgt ctggacgtga agttgcccaa aaccttatgt ctcattaata
    34621 tttaaaagat gatatcggat acgatatcat ctttttttct ttgtacgtga taggctcaaa
    34681 catagaaaaa agcacttcta atagaagtgc ttttaaaatt aatgctatgc ttattgagca
    34741 gcgtttgcgt ctttgagacc atattttttg ttgaaacggt ccacacgtcc atctgcttga
    34801 gtgaattttt gacgtcctgt atagaatgga tgtgaatctg atgagatttc cacacgaata
    34861 agtgggtaag tttcaccttc gaattctact gtttctttag ttgatttagt tgaaccacta
    34921 aggaatttgt aacctgtagt tgtatctaag aaaacaactg gacgataatc tggatggatg
    34981 tcttttttca tctttaaaaa tttcctttct gccataactc gtctgagtca tagttattaa
    35041 ccactctatt ctatcaaata agaatagtct tgacaagtgt tttgataaaa aaagtaaaat
    35101 tataaagaaa gtatttcctg gatttcttgg taaatagctt gattctcttc caaactataa
    35161 gaattagctc cgctggctaa aggatgtccg ccaccattat gacgtttagc tatttcatta
    35221 atgatgtgtg acttactacg taggcgcaca cgataatgcc catcgctttg ttcaacgaaa
    35281 attgcccaac tttctacaat atcaattttt ccaggtacac caacgattgc agcagtctct
    35341 gcatctgtta catcgaagcg ttttaggtct tcttgtgtta aagtaacgca tgcagcgccg
    35401 ttcttgtcaa ttttaagttg ctcaaaaata aagccttgta attttgcaat tttgaaagga
    35461 aaggaatcca tttgacgtgc cattgcagaa aaatcaaagt caaattcacg aagtttgcta
    35521 gcaatcttca gtgttttact tgtggtagca ggatataaaa agcgtcctgt atctcctaca
    35581 ataccattat atagaactct tgctgcactt gttgataaca acaaatcaca agaaagagca
    35641 aagtctgtca caatttcact tgcactagag gcgttagtat ctacataaga gagatctcca
    35701 tagacttcat cgtttggatg atggtcaatt ttaattaaaa agtcaccttt tttataacgt
    35761 tcatcatcaa tgcgtggggt atttgctgta tctgtaacaa caacaagagc gccttgatag
    35821 tcttggtcgg tcacttgatc cattttagca atccaagcga gagtgggttc gtcaaaacca
    35881 gtagcaagta cttttttctt tggaaaatta tgcctgatga tatcccttaa gccgatttgg
    35941 ctaccaagag catcagggtc tggtctcata tgtcgatgga taatgatggt gtcgtactct
    36001 ttgattttat caagaatttg ttgaaaaata atcatttttt acctacctaa ctgttttgtc
    36061 ttttagtgaa gaacaaggct atattgttga ctatattata gcttaaatct tttattgagg
    36121 aaagaaatta agatagcggt cacaatgttt gtttattttt atatgatggt atgttatttt
    36181 tttataaaat tcggatactg ctaactaata atctttttta taataatatt ttgtaaatgt
    36241 attgcttttt agctaacttt tgttaatata gtaaaaatgc tttttaatag gagataaatt
    36301 atgacacaag cagtattaaa agaattggct aaggcggagt tgcattgtca tctagatggc
    36361 tcactttctt taccagctat tagaaaattg gctaatatgg ctgatataat actgccgagt
    36421 agtgataaag aattaagaaa atatgttata gctccagctc aaacagaaag cttggtagat
    36481 taccttaaaa cgtttgagtt tatcagaccg cttcttcaaa cgaaggaagc attacgtttt
    36541 gctgcttatg atgttgctag acaggcagct ttagaaaatg ttatttatat tgagattcgt
    36601 tttgcgccag aactctctat ggataaaggt ttaactgcaa gtgatacagt ccttgcagtc
    36661 ttagaaggtt tagcagacgc tcagaaagaa tttaatatcg ttgcacgcgc ccttgtttgc
    36721 ggtatgcgcc aatcgtctca taaaacgacc aaggacatta ttaaacacat tgttgatttg
    36781 gctcccaaag gcctggtagg ttttgatttt gcaggtgatg aattttccta tccaacagat
    36841 agtttagtag atcttattca ggaagtaaaa cgctcaggtt acccgatgac tttacatgca
    36901 ggtgagtgtg gttgtgctaa gcatattgcg gatagtttaa atttagggat taagcgtatg
    36961 ggacatgtga cagctttaac aggacagagg gatttaataa agcgatttgt cgaagaagat
    37021 gctgtagcag agatgtgttt aactagcaat ttacaaacaa aagcagcctc aagtattcaa
    37081 agttttcctt atcaagaact ttatgatgct ggaggtaaga ttacaattaa tacagataat
    37141 cgtactgttt ccgatacaaa tttgacgaaa gaatatagtc ttttcgtaac ttattttgga
    37201 acgaaaatag aagactttct tgttttcaat caaaacgctg tgaaggcctc ctttacaagt
    37261 gattcagaga aagatacttt actacataag ctacaagaaa actatgacag ctatctaaaa
    37321 taaccttcac gattctttaa aaatatgata gaatagtggt aatttacaag tggaggaaga
    37381 tatggcttta gcaaagattg tttatgctag catgaccgga aatactgaag aaattgctga
    37441 cattgttgct gataaattac gtgacttggg tctagatgtt gaagtagaag agtgtacgat
    37501 ggttgatgct gcagattttg aagatgctga cattgcaatt gttgccactt atacatatgg
    37561 tgatggagat ttaccagatg aaatcgtgga cttttatgaa gatttagcag aggttgattt
    37621 atctggtaaa gtttacggcg tagtgggttc aggtgatact ttctatgatt acttctgcaa
    37681 atctgttgat gagttcgagg ctcaattcgc attaacagga gcacaaaaag gtgctgattg
    37741 tgttaaagtt gatttagcgg cagaagacga agacattgaa aacttagaag cttttgctga
    37801 agaaattgct tctaaactta actaagctac caaaaagacg aacacgagtc gtctttttaa
    37861 cttttttacc atcttttgta actagaatat gagatataga tatggaatta gaagagattc
    37921 gtcaagaaat agatgaaata gatcagcaat tagtaagcct tttagaaact agaatgggtc
    37981 taattttaga ggtcatagca tttaaaaaga aacatcgact tccagtttta gataataatc
    38041 gtgaaaacga agtgcttaac aatgtattga agaaagtcca aaaccatcaa tttgacgatg
    38101 tgatacgagc aactttcaaa gacattatga cagaatctcg tgtttatcaa aaggagaata
    38161 ttgtagacgg tgattaaacg atttgataat tatgctcagt acgtacttgt tttaggtttt
    38221 agcttactaa ttggtggtgt ggttggggct attacagcag tctttggacg tgttctatta
    38281 ttcctgacag cctttagaag tgattatata gcttacctac tcccttttct tagcattgtt
    38341 ggtttattta tagtttttgt ctatcaaaaa tttgggggta agagtgttaa agggatggga
    38401 ttggtatttg aagtaggtca tggtaatgag gagactattc cgaaacgtct tgttcctctc
    38461 gtgatattaa caacttggtt aacccactta tttggtggta gtgcaggtcg tgagggggtt
    38521 gctgtacaga ttggggctac cgtttcacac tactttcaaa aatattgccg tttacaaaac
    38581 gcttctcaac ttttcctagt aatgggaatg gcagcaggtt ttgctggttt atttcagact
    38641 cctttagctg ctactttttt tgcgattgaa gttttggtag taggtcgttt aatggtttcg
    38701 tatgtattac caagtttaat tgctgccctg acagcaaatt tcgtctcaca ttctttaggt
    38761 ttagaaaaat tttctcatag tatagcaaca tcaatggcgt taacaccaga tattattcta
    38821 aaattattgg ttttaggact atgttttggt ctgtgtggta atttatttgc ttatttattg
    38881 gctaaagcta aattaattgc ttctagtagg ttgctaaacc catataaacg gattttcaca
    38941 cttggattgt tagctacttt tttattgttt atcttccatt ttggacgata ttcaggactg
    39001 ggaacaaatc taattgaggc tagcttcact aataagaacc tttatgatta tgattggttg
    39061 ttaaaattgt gtttgacagt tattactttg gctgcaggtt atcagggagg agaagtaact
    39121 cccttatttg caatcggagc aagtttagga gtgattattg cgcccatttt aggtttgcca
    39181 gttattttag ttgctgcttt aggctatact agcgtttttg ggagtgcaac gaatactctg
    39241 ttaggtccta tcttaatagg aggcgaagtc tttggctttg ccaacactcc ttattttgtt
    39301 attgtatgtc ttgttgctta ctccatatca catgctcata cgatttatgg tgctcaatct
    39361 agatagttag tgatgagttt taaaagagtt tatcagtttg tagaacggaa tattccgttc
    39421 tcttttttat tctcttaaag tttataaaaa tggagtttgt caattttatg aatgcaaaaa
    39481 ttatgtggct aatataattt cttcaaagaa attgatttta tgattgtcat taaaatactg
    39541 taaaatccaa ttgaatgtgt aggaacttag gttaggataa tgtctacaag catttaagtc
    39601 gcgatggtga aatgcattat aagataaaaa gttgatgtgg atttcaactt tatgggaaag
    39661 agttaagtga cacgaaagct aatatagaaa acgtagctta aaaaatcaaa tattaaatga
    39721 attaaactct caaaaatcag aaaaaagcta acctagaaac agtaaatcaa attta
//
LOCUS       DATRMA010000010        37660 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.010, whole genome shotgun sequence.
ACCESSION   DATRMA010000010 DATRMA010000000
VERSION     DATRMA010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 37660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37660
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.010"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            <1..128
                     /locus_tag="VBC28_000474"
     CDS             <1..128
                     /locus_tag="VBC28_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HEO6814476.1"
                     /translation="QKRTVIFIQALELLGYISLNQDTTYRLNAKLDVEALKIYLT"
     gene            complement(157..1158)
                     /locus_tag="VBC28_000475"
     CDS             complement(157..1158)
                     /locus_tag="VBC28_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEO6814477.1"
                     /translation="MIQKNKAPFVLISSVIILLLLILVSISLGYANTSVIDVLKLISG
                     KSDDAFLFIITNIRLPRIIVCIFGGASLGIAGLLLQTLTKNPLADSGILGINAGAGLV
                     IALTIGTFNVSNPTILYFLPLFAMFGGLVTIFLIYLMSYRRNHNISPTRLIVTGIGIS
                     TIISGVMILIISQSNNQKMDMIVEWLSGKITISSWTTIITFIPILILLWGLAYSRSRH
                     LNIMNLNEQTALALGLHLKKERIYTLMLTSSLAAISVVLIGNITFIGLLAGHLSRRLL
                     GNNHKIILPSCLLIGAIILLVSDTIGRLLLVGTGIPTGLVVSIIGAPYFLWLMTKVD"
     gene            complement(1155..2180)
                     /locus_tag="VBC28_000476"
     CDS             complement(1155..2180)
                     /locus_tag="VBC28_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000471515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEO6814478.1"
                     /translation="MFKASKKLVQKNKSNHFWLVFFITLILFLIGCYASLRFGAINFK
                     TSDLITVLKNPLKNSNAQDVIFDIRLPRIIAAILVGAAMSQAGAIMQGVTRNAIADPG
                     LLGINAGAGLALVVAYAFLGSMHYSTILIVCLLGSVISCLLVFTLSYTKQKGYHQLRL
                     ILAGAMISTLFTSVGQVVTLYFKLNRTVIGWQAGGLSQINWKMLIIIAPIIILGLLIS
                     QLLAHQLTILSLNESVAKALGQKTQLMTAFLLLIVLFLSASSVALIGTVSFIGLIIPH
                     FIKLFIPKDYRLLLPLIGFSGATFMIWVDLSSRIINPPSETSISSIISIVGLPCFLWL
                     IRKGKNL"
     gene            complement(2196..3128)
                     /locus_tag="VBC28_000477"
     CDS             complement(2196..3128)
                     /locus_tag="VBC28_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEO6814479.1"
                     /translation="MKKIGIIVLTLLTFFLVSCGQQTKQESTKTTISKMPKIEGFTYY
                     GKIPENPKKVINFTYSYTGYLLKLGVNVSSYSLDLEKDSPVFGKQLKEAKKLTADDTE
                     AIAAQKPDLIMVFDQDPNINTLKKIAPTLVIKYGAQNYLDMMPALGKVFGKEKEANQW
                     VSQWKTKTLAVKKDLHHILKPNTTFTIMDFYDKNIYLYGNNFGRGGELIYDSLGYAAP
                     EKVKKDVFKKGWFTVSQEAIGDYVGDYALVNINKTTKKAASSLKESDVWKNLPAVKKG
                     HIIESNYDVFYFSDPLSLEAQLKSFTKAIKENTN"
     gene            complement(3152..3946)
                     /locus_tag="VBC28_000478"
     CDS             complement(3152..3946)
                     /locus_tag="VBC28_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000018231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6814480.1"
                     /translation="MSHIKAENIIVSYDQKEIINNLSLSILNQKITTIIGANGCGKST
                     LLKALTRIHKIKDGTITIDGHDIAHLPTKEIAKKIALLPQVLEATEGITVYELISYGR
                     FPHQKYLGNLTNDDRSKIHWAMEMTNVAQFANRDVDDLSGGQRQKVWIAMALAQDTDT
                     IFLDEPTTYLDMNHQLEVLELLKKLNDETQKTIIMVLHDLNLSARYSDYLVAMKTGKI
                     IYEGSPSQIMTKDIIKDIFKIDAHIIQDPISKQPVLLSYQLSTDQN"
     gene            complement(4102..5556)
                     /locus_tag="VBC28_000479"
     CDS             complement(4102..5556)
                     /locus_tag="VBC28_000479"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HEO6814481.1"
                     /translation="MITIDKILEILKNDHNFREILFHEHYYYNWTQNVTFNALSYDSR
                     QISSDTLFFAKGATFKKEYLDSAITAGLSFYVSEIDYGADIPVILVNDIKKAMSLISM
                     SFYNNPQNKLKLLAFTGTKGKTTAAYFAYHMLKVNHRPAMLSTMNTTLDGKSFFKSHL
                     TTPESLDLFRMMATAVENQMTHLIMEVSSQAYLTKRVYGLTFDVGVFLNISPDHIGPI
                     EHPTFEDYFFHKRLLMENSNAVVVNSQMDHFNIVKEQVEYIPHDFYGDYSENVITESK
                     AFSFHVKGKLENTYDIKLIGKFNQENAIAAGLACLRLGVSIEDIKNGIAQTTVPGRME
                     VLTQTNGAKIFVDYAHNGDSLKKLLAVVEEHQKGDIILVLGAPGNKGQSRRKDFGDVI
                     NQHPNLQVILTADDPNFEDPLVISQEIASHINRPVTIIIDREEAIANASTLTNCKLDA
                     IIIAGKGADAYQIIKGNRDNYSGDLEVAKKYLKR"
     gene            5680..7314
                     /locus_tag="VBC28_000480"
     CDS             5680..7314
                     /locus_tag="VBC28_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEO6814482.1"
                     /translation="MSQKTTKVSQQEQMVKGTAWLTAGNFISRLLGAIYIIPWYAWMG
                     KHAAEANALFGMGYEIYALFLLISTVGIPVAVAKQVSKYNTLGKEEMSIYLVRKILQF
                     MLILGGIFALIMYIGSPLFASLSKGGQELVPILRSLTLAVLVFPSMSVLRGFFQGFNN
                     LKPYAISQVAEQIIRVIWMLLTAFYIMRLGSGDYIAAVTQSTFAAFVGMFASIAVLLY
                     FLWRYNMLSALIGKTPKHIKLDTKEILIETIKEAIPFIITGAAIQIFKLIDQFSFGNT
                     MALFTNYSSEELRVMFAYFSSNPGKVTMILIAVATAIAGVGIPLLTENFVKNDKKAAA
                     RLVVNNLQMLLMFLLPAVAGSVILAKPLYTVFYGLPQGQALGLFVISLIQTIILSIYT
                     VLAPMLQALFENRKAIIYFLYGLVAKVILQLPSIFLFHAYGPLFSTTVALCIPVILMY
                     LKIHEITGFKRQAIRRTSALVLILTLLMSFIISMIIWLMNLVIVPDSRLVSLVYIIVI
                     GAIGLGVYGFMALATHLLDKMIGSRAQDLRRKLHLN"
     gene            7449..8669
                     /gene="pepT"
                     /locus_tag="VBC28_000481"
     CDS             7449..8669
                     /gene="pepT"
                     /locus_tag="VBC28_000481"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HEO6814483.1"
                     /translation="MSYEKLLERFLTYVKINTRSNPNSTQTPTTQSQVDFALTVLKPE
                     MEAIGLKDVHYLPSNGYLVGTLPATSDRLRHKIGFISHMDTADFNAENITPQIVDYKG
                     GDIELGDSGYILSPKDFPNLNNYHGQTLITTDGKTLLGADDKSGIAEIMTAMEYLASH
                     PEIEHCEIRVGFGPDEEIGIGADKFDVKDFDVDFAYTVDGGPLGELQYETFSAAGLEL
                     TFEGRNVHPGTAKNQMINALQLAMDFHSQLPENERPEQTDGYQGFYHLYDLSGTVDQA
                     KSSYIIRDFEEVDFLKRKHLAQDIADNMNEALQSERVKVKLYDQYYNMKKVIEKDMTP
                     INIAKEVMEELDIKPIIEPIRGGTDGSKISFMGIPTPNLFAGGENMHGRFEFVSLQTM
                     EKAVDVILGIVAKD"
     gene            8698..9189
                     /locus_tag="VBC28_000482"
     CDS             8698..9189
                     /locus_tag="VBC28_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HEO6814484.1"
                     /translation="MIKLFGKIRYHWQPELSWAIIYWSIAIAPIFIGLSLLYERTEIP
                     SQVFVLFAIFIVLVGIGFHRYFVIEEDGYLRIVSFNFLRRTKFPIEDIAKIEVTKSSV
                     TIKFNNNHERIFYMRKWPKKYFLDALAIEPTFKGEVELLDNLIKMDYFECYRYDKKAL
                     TKS"
     gene            complement(9176..9373)
                     /locus_tag="VBC28_000483"
     CDS             complement(9176..9373)
                     /locus_tag="VBC28_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEO6814485.1"
                     /translation="MKVALIPEKCIACGLCQTYSNIFDYQDDGIVKFSDTDNLEKEIP
                     SSDQDTVLAVKSCPTKALTIL"
     gene            9419..9943
                     /locus_tag="VBC28_000484"
     CDS             9419..9943
                     /locus_tag="VBC28_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001085531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEO6814486.1"
                     /translation="MAKKPWEKKVVENNSHRKDKITRTSRGVVSSTPWITAFLSAFFV
                     IVVAILFIVFYTSNRGEDRAKETSGFYGASSQKVNSSKTKKASTSKKTTSSSKTKTSD
                     KSTTSSSTVASSSETSTSSSVNGGTIVVQPGEGAASIAARAGISVARLQELNPSHMTS
                     GYWYANPGDPVVVN"
     gene            9954..10637
                     /locus_tag="VBC28_000485"
     CDS             9954..10637
                     /locus_tag="VBC28_000485"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEO6814487.1"
                     /translation="MNSINIAIDGPASSGKSTVAKIIAKNLNYTYLDTGAMYRCATYL
                     ALQHGYEAQDVSKILGLLAERPISFGKAEDGSQTVFLGTEEVTLAIRQNDVTNNVSWV
                     SAIPEIREELVNQQRRIAKDGAIIMDGRDIGTVVLPDAELKIFLVASVDERAERRFKE
                     NQEKGIESDFETLKSEIAARDYKDSHREVSPLKAAEDAIEFDTTGVSIEGVVTFIQEK
                     AEKIIDMKN"
     misc_feature    10658..10783
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            10798..11328
                     /gene="infC"
                     /locus_tag="VBC28_000486"
     CDS             10798..11328
                     /gene="infC"
                     /locus_tag="VBC28_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011226065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEO6814488.1"
                     /translation="MKIIAKKDLFINDEIRVREVRLVGLEGEQLGIKPLSEAQAIADD
                     ANVDLVLIQPQATPPVAKIMDYGKFKFEYQKKQKEQRKKQSVVTVKEVRLSPVIDKGD
                     FETKLRNGRKFLEKGNKVKVSIRFKGRMITHKEIGAKVLAEFAEATQDIAIIEQRAKM
                     DGRQMFMQLAPIPDKK"
     gene            11368..11568
                     /gene="rpmI"
                     /locus_tag="VBC28_000487"
     CDS             11368..11568
                     /gene="rpmI"
                     /locus_tag="VBC28_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEO6814489.1"
                     /translation="MPKQKTHRASAKRFKRTGSGGLKRFRAFTSHRFHGKTKKQRRHL
                     RKATMVSSGDFKRIKAMLTRLK"
     gene            11626..11985
                     /gene="rplT"
                     /locus_tag="VBC28_000488"
     CDS             11626..11985
                     /gene="rplT"
                     /locus_tag="VBC28_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019786714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEO6814490.1"
                     /translation="MARVKGGVVSRKRRKRVLKLAKGYYGAKHILFRTAKEQVMNSYY
                     YAYRDRRQKKRDFRKLWITRINAAARMNGLSYSQLMHGLKLAEIEVNRKMLADLAVND
                     AAAFTALADAAKAKLGK"
     gene            complement(12227..14371)
                     /locus_tag="VBC28_000489"
     CDS             complement(12227..14371)
                     /locus_tag="VBC28_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEO6814491.1"
                     /translation="MKKIKDFASRAINTRLGFILLLVVIYWLKTIWAYHTDFNLGLEN
                     SYQLFLTIINPIPLGLLIIGLALYVKRTKAFYITAFITYAIVNILLIANAIYYREFSD
                     FITVSAVLASSKTSAGLGDSALNLLRIWDLVYVFDFIILIFLFATKKIHLDDRPFNKR
                     ASFSITALSGLLFSINLFLAEIDRPELLSRGFSNTYIVKALGLPSFSIYSGNQTYQAQ
                     KERNGATAQELATAKKYVAEHYAKPNPEYYGIGKGRNVIMIHLESFQQFLIDYKLNID
                     GKEHVVTPFINSLYHSKETVSFSNFFHQVKAGKTSDAETLMENSLFGLSSGSFMVNYG
                     GENTQFAAPHILAQNGGYSSAVFHGNVGTFWNRNNAYKQWGYDYFFDSSYFSKQTKDN
                     SFQYGLNDKYMFADSIKYLEHMQQPFYTKFITVSNHYPYTSLKGESDEEGFPLAKTND
                     ETINGYFATANYLDTALKSFFEYLKAAGVYDNSIIVMYGDHYGISNTRNPSLAELLGK
                     DPETWSEYDNAMLQRVPYMIHIPGYSKGFISNTYGGEVDNLPTLLHILGIDTSKYTQL
                     GQDLLSKDNKQMVAMRTTGQYITPKYTNYSGHLYYTDSGQEITNPDETTKAEIKAIRD
                     ATNKQLSTSDSIQTGDLLRFDENNGLKTVEVEKFNYTHSLKALKAKERKLKDRSTSIY
                     SKHNNKSTVDLFHAPSYLELQDPNKTHKTSKK"
     gene            14506..15663
                     /locus_tag="VBC28_000490"
     CDS             14506..15663
                     /locus_tag="VBC28_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEO6814492.1"
                     /translation="MNKLKVNSVVERKIKSGAQLLEKKDFDTSLVNQLVQLFSQSNQF
                     LGMAYLSPQNKGIGWLLSRQVFDFNHDYFVSLFEKSREKRQKFEKSSQTTAYRLFNQD
                     GDNFGGLTIDFYSDYALFSWYNEFVYTNRQMIVAAFKQVYPNIKGAYEKIRFKGLDFE
                     SAHLYGQEAPESFLILENNIKYSVFLNDGLMTGIFLDQHDVRKALATNLSEGKKVLNM
                     FSYTATFSVAAAVGGALETTSVDLAKRSRELSKAHFDANQIVTDNHRFIVMDVFEYYK
                     YAKRKHLSYDVIVIDPPSFARNKKQTFSVTKDYYKLIEQALDILTPGGTIIASTNAAN
                     LTVSQFKKQLEKGFGKASHNYISLQQLPEDFTINDKDQQSNYLKVFTIKVK"
     gene            15663..16340
                     /locus_tag="VBC28_000491"
     CDS             15663..16340
                     /locus_tag="VBC28_000491"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HEO6814493.1"
                     /translation="MKIVVPVMPRSLEEAQEIDLSKFDSVDIIEWRADALPKDDIINV
                     APAIFEKFAGHEIIFTLRTTREGGNIVLSDAEYVELIQKINSIYNPDYIDFEYFSHKE
                     VFQEMLEFPNLVLSYHNFQETPENIMEIFSELTALAPRVVKIAVMPKNEQDVLDVMNY
                     TRGFKTINPDQVYATVSMSKIGRISRFAGDVTGSSWTFAYLDSSIAPGQITISEMKRV
                     KALLDAD"
     gene            16434..17501
                     /locus_tag="VBC28_000492"
     CDS             16434..17501
                     /locus_tag="VBC28_000492"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001195728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEO6814494.1"
                     /translation="MQVEVDLPNHPYHIKIEEGCFSEAGDWVSHLWQKQMITIITDSN
                     VEILYGESLVNQLKKQGFTVHVFSFAAGEASKTLEVANRIYAFLAKHHMTRSDGIIAL
                     GGGVVGDLAAFVASTYMRGIHFLQIPTSLTAQVDSSIGGKTGVNTSFAKNMVGTFAQP
                     DGVLIDPVTLKTLGNRELVEGMGEVIKYGLIDDIKLWHILEEMDGTIDSILDNALAII
                     YHSCQVKRKHVLADQYDKGLRMHLNFGHTIGHAIEVHAGYGEIMHGEAVAIGMIQLSR
                     VAERKNLMPRGISQDIYNMCLKFGLPVHYAEWDKDVLFDILSHDKKASGQFIKIVILP
                     QLGSATVHQIPLEEMRDYLEK"
     gene            17502..18668
                     /gene="aroC"
                     /locus_tag="VBC28_000493"
     CDS             17502..18668
                     /gene="aroC"
                     /locus_tag="VBC28_000493"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEO6814495.1"
                     /translation="MRYLTAGESHGPSLTAIIEGIPAGLKLSAKDINEDLKRRQGGYG
                     RGNRMKIETDQVIISSGVRHGKTLGSPITLTVTNKDHSKWLDIMSVEDIEERLKQKRR
                     IKHPRPGHADLVGGIKYRFDDLRNALERSSARETTMRVAIGAIAKRILKEIGIEIANH
                     IVVFGGKEITVPDKLTVQQIKVLSSQSQVAIVNPSFEQEIKDYIDSVKKAGDTIGGVV
                     ETIVGGVPVGLGSYVHWDRKLDAKIAQAVVSINAFKGVEFGLGFKSGFLKGSQVMDSI
                     SWTKDQGYIRQSNNLGGFEGGMTNGEPIIVRGVMKPIPTLYKPLMSVDIDTHEPYRAT
                     VERSDPTALPAAGVVMEAVVATVLVTEVLEKFSSDNMYELKEAVKLYRNYVDHF"
     gene            18752..19087
                     /locus_tag="VBC28_000494"
     CDS             18752..19087
                     /locus_tag="VBC28_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF/YmcA family competence regulator"
                     /protein_id="HEO6814496.1"
                     /translation="MANVYDLANELERAVRALPEYQAVLTAKSAIESDADAQVLWQDF
                     LATQSKVQEMMQSGQMPSQEEQDEMSKLGEKIESNDLLKVYFDQQQRLSVYMSDIEKI
                     VFAPMQDLM"
     gene            19262..20611
                     /gene="gorA"
                     /locus_tag="VBC28_000495"
     CDS             19262..20611
                     /gene="gorA"
                     /locus_tag="VBC28_000495"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HEO6814497.1"
                     /translation="MSKQYDYIVIGGGSAGSGTANRAAMYGAKVLLIEGGQVGGTCVN
                     LGCVPKKIMWYGAQVSETLHKYSSGYGFEVNNLNFDFTTLKANRDAYVQRSRQSYAAN
                     FERNGVEKIDGFARFIDNHTIEVNGQQYKAPHITIATGGHPLYPDIIGSELGETSDDF
                     FGWETLPDSILIVGAGYIAAELAGVVNELGVETHLAFRKDHILRGFDDMVTSEVMAEM
                     EKSGISLHANHVPKSLKRDEGGKLIFEAENGKTLVVDRVIWAIGRGPNVDMGLENTDI
                     VLNDKGYIKADEFENTSVDGVYAIGDVNGKIALTPVAIAAGRRLSERLFNHKDNEKLD
                     YHNVPSVIFTHPVIGTVGLSEAAAIEQFGEDNIKVYTSTFTSMYTAVTTNRQAVKMKL
                     ITLGKEEKVIGLHGVGYGIDEMIQGFSVAIKMGATKADFDDTVAIHPTGSEEFVTMR"
     gene            complement(20654..21106)
                     /locus_tag="VBC28_000496"
     CDS             complement(20654..21106)
                     /locus_tag="VBC28_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6814498.1"
                     /translation="MSKGYSRQQKKSTKTNTDSTQSPAKHIKTGFSALQKTIALVGSV
                     LSIIVASITITNAINNHKDSSKTDSSTSKTVIIRESGQNSSNQVSTEKDETRASSSQT
                     LENSSASSTPSSSSNSAANNDQTSSSTNNDQTNNTTTDTSTIENTTTP"
     gene            21353..22498
                     /locus_tag="VBC28_000497"
     CDS             21353..22498
                     /locus_tag="VBC28_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEO6814499.1"
                     /translation="MIYFDNSATTIPYPEVLRTYQEVASKIYGNPSSLHELGTTSSRI
                     LEASRKQIASLLELKANEIFFTSGGTEADNWVIKGLAFEKQHFGNHIIVSDIEHPAVK
                     ESAKWLGEYGFEIDYAPVDDKGFVDVEALVKLIKPETILISIMAINNEIGSIQPIKAI
                     SDLLSDKPTISFHVDAVQAIGKIPTKDYLTERVDFASFSSHKFHGVRGVGFLYIKEGK
                     RISPLLTGGGQETDLRSTTENVAGIAATAKALRMVMDKEVVAIPKISKMKTIIHDELA
                     KYEDITLFSGKEDFSPNIITFGIKGVRGEVLVHAFEGHDIFISTTSACSSKAGKPAGT
                     LIAMGISTKLAQTAVRISLDDDNDMGQVEQFLTIFKQVYEKTKKVRG"
     gene            22500..23714
                     /gene="thiI"
                     /locus_tag="VBC28_000498"
     CDS             22500..23714
                     /gene="thiI"
                     /locus_tag="VBC28_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HEO6814500.1"
                     /translation="MQYSEIMIRYGELSTKKKNRMRFINKLKNNMEHVLSIYPDVSVK
                     TDRDRGHVYLNGTDYHEVAESLKEIFGIQAFSPSFKVEKNVDTLVKAVQEIMTSVYKD
                     GMTFKITAKRSDHSFELDSRALNHTLGDAVFSVLPNIKAQMKQPDINLKVEIRDEAAY
                     ISYEDIRGAGGLPVGTSGKGMLMLSGGIDSPVAGYLALKRGVDIEAVHFSSPPYTSPG
                     ALKKAHDLTRKLTKFGGNIQFIEVPFTEIQEEIKAKAPEAYLMTLTRRFMMRITDRIR
                     EDRNGLVIINGESLGQVASQTLESMQAINAVTATPIIRPVVTMDKLEIIDIAQKIDTF
                     DISIQPFEDCCTIFAPDRPKTNPKIKNTEQYEKRMDVEGLVERAVAGIMVTTIQPQAD
                     SDDVDDLIDDLL"
     gene            23816..24994
                     /locus_tag="VBC28_000499"
     CDS             23816..24994
                     /locus_tag="VBC28_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000101234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEO6814501.1"
                     /translation="MARIPYQKTLIFCVAVIIAIFILGLSKDLAQSKGQKVANNNTVK
                     TARVVANGDILLHDVLYASARQPDGTYNFTPYFKEVKSWIESADLAIGDYEGTISSEY
                     PLAGYPLFNAPNEIATTMKETGYDVVDLAHNHILDSQLAGAINTVKTFNRLGLDTIGV
                     YLKDRNKEDILIKHVNGIKIAILGYSYGYNGMEANVSKSDYEKHMSDLDTKKIKQDIK
                     KAEKEADITIVMPQMGIEYQKKPTTEQVMLYHSMIKWGADIIFGGHPHVVEPSEVIKK
                     DGQKKFIIYSMGNFISNQRLETVDDIWTERGLLMDVTIEKKGQKTVIKKVKAHPTLVE
                     AKPNGRYVEEGFPAYDYRTLVLEDYIEGGKKRSTIDQTMKDKVDKAYKEMNELVNLKW
                     "
     misc_feature    25070..25149
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            25184..25498
                     /gene="rplU"
                     /locus_tag="VBC28_000500"
     CDS             25184..25498
                     /gene="rplU"
                     /locus_tag="VBC28_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEO6814502.1"
                     /translation="MSTYAIIKTGGKQVKVEVGQAIYVEKLDVEAGAEVTFNEVVLVG
                     GETTKVGTPVVEGATVVGTVEKQGKQKKVVSYKYKPKKGSHRKQGHRQPYTKVVINAI
                     NA"
     gene            25505..25843
                     /locus_tag="VBC28_000501"
     CDS             25505..25843
                     /locus_tag="VBC28_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HEO6814503.1"
                     /translation="MIKATFTRNQSGYLYSAEISGHAGSGEYGFDVICAAVSTLSINF
                     INSLEALTTCQAQLIINDVEGGYMKIDLSSIPQHKEDKVQLLFESYLLGMTNLSKDSS
                     EFVSTVVMTH"
     gene            25865..26158
                     /gene="rpmA"
                     /locus_tag="VBC28_000502"
     CDS             25865..26158
                     /gene="rpmA"
                     /locus_tag="VBC28_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEO6814504.1"
                     /translation="MLKMNLANLQLFAHKKGGGSTSNGRDSQAKRLGAKAADGQTVSG
                     GSILYRQRGTHIYPGANVGRGGDDTLFAKVEGVVRFERKGRDKKQVSVYPIAK"
     gene            26374..27279
                     /locus_tag="VBC28_000503"
     CDS             26374..27279
                     /locus_tag="VBC28_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEO6814505.1"
                     /translation="MNIQQLRYVVAIANSGTFREAAAKLFVSQPSLSVAVRDLETELG
                     FQIFTRTTTGAVLTNQGMTFYENALEVVKSFDSFEKQFSQSEATEQEFSIASQHYDFL
                     PPLITAFSKCNDNFSYFRIFESTTIRILDEVAQGNSEIGIIYINSQNKKGLLQRLDKL
                     GLEFVELIPFKTHIYLGKDHPLASKTSLIMTDLEGLPTVRFTQDRDDYRYYSENFVEV
                     LDSSVTYNVTDRATLNGILERTQAYATGSGFLDSRSVNGITVIPLEDHLDNQMIYIKR
                     KDRNLSQMALKFVAVMEEYFKGFNS"
     gene            27288..27752
                     /locus_tag="VBC28_000504"
     CDS             27288..27752
                     /locus_tag="VBC28_000504"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEO6814506.1"
                     /translation="MRKIIIPIITILLIALDQLSKLWIVKHIELNQIKEFIPNIVSLT
                     YLRNYGAAFSILQNQQWLFTLITIFVVGVAIIYLMKHINGSYWLLISLTLIISGGLGN
                     FIDRLRLGYVVDMVHLDFINFAIFNVADSYLTIGIICLMIALWKEESNGNHN"
     gene            27736..28626
                     /locus_tag="VBC28_000505"
     CDS             27736..28626
                     /locus_tag="VBC28_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEO6814507.1"
                     /translation="MEITIKIAGVRLDKALADNSELSRSQANEEIKKGIVLVNGQVKK
                     AKYTVQEGDRITFDIPKEEVLDYQAENIPLDIIYQDDDVAVVNKPQGMVVHPSAGHSS
                     GTLVNALMYHIKDLSSINGVVRPGIVHRIDKDTSGLLMVAKNDRAHQVLAEELKDKKS
                     LRKYLAIVHGNLPNDRGVIEAPIGRSDKDRKKQTVTAKGKPAITRFHVLERFGDYTLV
                     ELSLETGRTHQIRVHMAYIGHPLAGDPVYGPRKTLGGKGQFLHAQTLGFTHPSNGENL
                     IFSVEVPEIFQTTLEKLRKN"
     gene            28802..29323
                     /gene="pyrR"
                     /locus_tag="VBC28_000506"
     CDS             28802..29323
                     /gene="pyrR"
                     /locus_tag="VBC28_000506"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEO6814508.1"
                     /translation="MKRKEIIDDVTMKRAITRITYEIIERNKNLDNIVLAGIKTRGVF
                     LAKRIQERLKQLENLDIPVGELDTKPFRDDMKVEVDTTTMPVDITDKDIILIDDVLYT
                     GRTIRAAIDNLVSLGRPSRVSLAVLIDRGHRELPIRADYVGKNIPTSQFEEILVEVME
                     HDGYDRVSIIDPS"
     gene            29372..30442
                     /locus_tag="VBC28_000507"
     CDS             29372..30442
                     /locus_tag="VBC28_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HEO6814509.1"
                     /translation="MAKKLLILEDGTVFEGLSFGSSLDVTGELVFCTGNTGYQEIITN
                     PSHNGKILVFTSPLIGNYGIHRSYSEAIIPTCLGVVVAEYSRCVSSDTSKMNLDEFLK
                     MKKVPAMSGVDTRYLMQVIKEKGFVKATLAEAGDVLSHLQDQLIATVLPTNNVEQVST
                     KTAYPSPASGRNIVVLDFGLKHSILRELSKRQCDVTVIPYNTSLEGIKNLYPEGIILS
                     NGPGNPEKLQEILNTIKELQKSVPMLGIGLGHQLIAMANGAEIMRLPVAKKGPNYPMR
                     DIATGRLETVSQFNHFTVNRLNLPHDLLVTHEGLNDQEIVALRHRSFPVMSVQFYPEA
                     APGPHDVTYFFDEFLEMIGSRA"
     gene            30498..32096
                     /locus_tag="VBC28_000508"
     CDS             30498..32096
                     /locus_tag="VBC28_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase large subunit"
                     /protein_id="HEO6814510.1"
                     /translation="MLNTKDKQPILLIIFGQKMKEYDDLIRSTVERLSKRDRPVSILI
                     SKELNHVIHHYDAQFENLETLVGYINTHEDSLIYPLCLDKNHELFQEFLAWQSHYSRQ
                     YISDNLSEKSVQAIDNNQDFVQVDCLVMRDSLNNCLYVSDLEYIESNKTTGKSLAIVP
                     SQTLSDAARQTIRDVAFDVCRKANIIGVCYFSFLIDLNSLDYHIISLSSGLSHQSILF
                     ETITTYPVLEIATKLTVGYTFSQLKHSYYPNTSAFLEPQLDYVATVSFSFEKVDYIFF
                     ARNIEQLFLNLLEASSHDHFPFLSDISEEDLMFALIQKKENRLAYLLEAFRRGFDLYD
                     LSSVTKINPFYLDKCLHIVELYENLNKSQYNVDIYKEAKRYGFSDDYIASSWQISLID
                     MLEYRKKHSVAPVLKQVEQSSGVLTGHQIQYFRSYDWHSDYISSGCQKALIMVDKGYS
                     LVKLNELIKQIKQTHLELLIVTNQPLLIEQLNDTSIIFDTIGIETILTIMGIEEVEEV
                     YLLGESQADMFSKMKNFSISCQSI"
     gene            32298..33542
                     /locus_tag="VBC28_000509"
     CDS             32298..33542
                     /locus_tag="VBC28_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEO6814511.1"
                     /translation="MSKRQNLGISKKGAIISGLSVALIVVIGGFLWVQSQPNKSAVKT
                     NYKVFNVREGSVSSSTLLTGKAKANQEQYVYFDANKGNRATVTVKVGDKITAGQQLVQ
                     YDTTTAQAAYDTANRQLNKVARQINNLKTTGSLPAMESSDQSSSSSQGQGTQSTSGAT
                     NRLQQNYQSQANASYNQQLQDLNDAYADAQAEVNKAQKALNDTVITSDVSGTVVEVNS
                     DIDPASKTSQVLVHVATEGKLQVQGTMSEYDLANVKKDQAVKIKSKVYPDKEWEGKIS
                     YISNYPEAEANNNDSNNGSSAVNYKYKVDITSPLDALKQGFTVSVEVVNGDKHLIVPT
                     SSVINKDNKHFVWVYNDSNRKISKVEVKIGKADAKTQEILSGLKAGQIVVTNPSKTFK
                     DGQKIDNIESIDLNSNKKSEVK"
     gene            33545..34255
                     /locus_tag="VBC28_000510"
     CDS             33545..34255
                     /locus_tag="VBC28_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6814512.1"
                     /translation="MTTNRKELIRLHQIVKSYQNGDQKLQVLKNIDLTVYEGEFLAIM
                     GPSGSGKSTLMNIIGLLDSPTSGDYSLNGKRVEELSQTKLAQVRNKEIGFVFQQFFLL
                     SKLTALQNVELPLIYAGVPPKKRKNLAKQFLDKVELRERMNHLPTELSGGQKQRVAIA
                     RALVNSPSIILADEPTGALDTKTGEQIMQFLTELNQEGKTIIMVTHEPEIADYATRKI
                     VIRDGEITADTTDSIRID"
     gene            34270..35517
                     /locus_tag="VBC28_000511"
     CDS             34270..35517
                     /locus_tag="VBC28_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000432854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6814513.1"
                     /translation="MENWKFALSSILGHKMRAFLTMLGIIIGVASVVLIMALGKGMKD
                     SVTNEITKSQKNLQIYYKTKEDQKNEDNFGAQGAFMQGSDTNRKEPIIQESWLKKIAK
                     EVDGVSGYYVTNQTNAPVAYLEKKAKTVNITGINRTYLGIKKFKIKSGRQFQEEDYNQ
                     FSRVILLEEKLAQRLFQTNEAALNKVVTVKNKSYLVVGVYSDPEAGSGLYGSNSDGNA
                     ILTNTQLASEFGAKEAENIYFHLNDVSQSNRIGKEIGKRLTDISHAKDGYYDNFDMTS
                     IVKSINTQVGIMTGVIGAIAAISLLVGGIGVMNIMLVSVTERTREIGLRKALGATRRK
                     ILAQFLIESMVLTILGGLIGLLLAYGGTMLIANAQDKITPSVSLNVAIGSLIFSAFIG
                     IIFGLLPANKASKLNPIDALRYE"
     gene            35646..35918
                     /gene="rpsP"
                     /locus_tag="VBC28_000512"
     CDS             35646..35918
                     /gene="rpsP"
                     /locus_tag="VBC28_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEO6814514.1"
                     /translation="MAVKIRLTRMGSKKKPFYRINVADSRAPRDGRFIETVGTYNPLV
                     AENQVTIKEERVLEWLSKGAQPSDTVRNLLSKAGVMTKFHDQKFSK"
     gene            35928..36170
                     /locus_tag="VBC28_000513"
     CDS             35928..36170
                     /locus_tag="VBC28_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEO6814515.1"
                     /translation="MDTIENLIIAIVKPLISQPDQLTIKIQDGPEFLEYHLDLDTQDI
                     GRVIGKKGRTITAIRSIVYSVPTQGKKVRLIIDEKE"
     gene            36302..>37660
                     /locus_tag="VBC28_000514"
     CDS             36302..>37660
                     /locus_tag="VBC28_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO6814516.1"
                     /translation="MLFDFLEEKIKLKAYLMTALLDDGEINLDHFKTEYALSEKILQG
                     LISELQILYSDFYLVVKSRTMVLFHYETLSKLETLHTIYRESNTLQFLAYFIAPQNVS
                     FSEFTAKKFISVASAYRIKKNCKLYLESVKLSIQKNKVTGPEYQIRLLIALLQYHFGF
                     IIYPFDATSEVMIRDFIEYSNFKGCDYDLKDLSEEYYYFYILVNLVWKRKEYKVEIPQ
                     TESFNQLKKSILYMELSKAAQKSIAIHLGIELNEGLLDYLYLVFCISDGPLFSNRDDI
                     NINEEFIFASYKISKSKAFKNFCGRLLGREFIDSQHFKDINAYFLRHFICYCYYFIPD
                     FYFLNTSRLSYSKDLYHLLDKGLADIFNLKGGNLTFSKHETVLLTMQLSNLIETFLAP
                     LSVYVISSSNIRLQTYQVMLNQYFTSKIAEFFFVNYQTTQIDEKLLKKADIIIAERRY
                     ISS"
BASE COUNT        12459 a         5800 c         7316 g        12085 t
ORIGIN      
        1 agcaaaaacg tacagttatt tttatccaag ctttagaatt acttggatat attagcttaa
       61 accaagatac tacctatcgt ttaaatgcta aacttgatgt tgaagcttta aagatttact
      121 taacataaaa aagactggaa gtttgtccag tcttttttag tcaacttttg tcatcaacca
      181 gagaaaatag ggggcaccaa ttattgatac aaccaatcca gtgggaatac ccgtaccaac
      241 taagaggaga cgacctatag tatctgatac caataggata atagcaccaa taagaagaca
      301 gctaggtagt ataattttat ggttatttcc taatagtcgt ctacttaagt gcccagcgag
      361 taagcctata aaagtaatat taccaattaa gacgacgcta atagcagcga ggctacttgt
      421 cagcatcaaa gtatatattc tttccttttt taaatgaagt cctagagcga gagcagtctg
      481 ctcgtttagg ttcataatat taaggtggcg actgcgacta tatgctaatc cccataacaa
      541 aatgagaatc gggataaaag tgataatggt agtccaggaa ctaattgtaa tttttccact
      601 aagccattca acgatcatat ccattttttg attattagac tgactaataa tcaaaatcat
      661 aacgcctgaa ataattgtag atattccgat accagtgacg attaatcgtg tagggctaat
      721 attgtgattt cttctgtatg acataagata gataagaaaa atagttacca accctccaaa
      781 catagcaaat aggggtaaaa agtataatat agttggattg ctaacattaa atgttcctat
      841 ggttagagcg attactaatc cagctccagc attgatacca agaataccgg aatcagctag
      901 tggatttttg gttaacgtct gtaatagcaa cccagcaatt cctaaagaag ctcccccaaa
      961 gatacagaca ataatacgag gtaaacgaat gttagtaatg ataaaaagga aggcatcatc
     1021 agattttcca cttattagtt ttaaaacgtc aataacggaa gtattagcat agccaagtga
     1081 aattgatact agtattaata atagtaggat aatgacagat gatattaaaa caaaaggagc
     1141 tttatttttt tgaatcatag atttttccct tttctaatta accataggaa acatggaagc
     1201 cctacgatac tgattataga actaattgat gtctctgaag ggggatttat aatacgagat
     1261 gataagtcta cccagatcat gaaagtagcc cctgaaaatc ctattaaagg taataacaga
     1321 cgataatctt tgggaataaa aagcttaata aaatgtggaa taatcaatcc aatgaaagaa
     1381 acggttccta tcaaagcgac tgatgaagct gataaaaaca aaacaataag taatagaaac
     1441 gcagtcatta attgagtttt ttgtcccaat gctttggcca cagattcatt aaggctcaag
     1501 atagttaatt gatgtgctag gagttgtgaa ataagtaaac ctaaaataat gataggtgct
     1561 attatgatta gcattttcca gttaatttgg gataaaccac ctgcttgcca accgataaca
     1621 gttcgattga gtttaaagta taatgtaacc acttgtccaa cagacgtaaa aagtgtggag
     1681 atcatcgctc cggctaaaat gagtcgtagt tggtggtaac ccttttgctt tgtataagag
     1741 agtgtgaaga ctaaaagaca agaaatgaca gaccctagta agcagactat gagaatcgta
     1801 ctatagtgca tgctccctaa aaaagcatat gctacaacaa gggctaaccc agctccggcg
     1861 ttaataccta gtagtccagg atcagcaatg gcgtttcgag tgactccctg cataatagca
     1921 cctgcttgtg acatagctgc acctactaag atagctgcaa ttatgcgtgg tagtctaatg
     1981 tcaaaaataa catcctgagc attagagttt tttaagggat tttttaaaac agttattaaa
     2041 tcactagtct tgaaattgat agcaccaaat cgtaagctgg cgtaacaacc aataagaaat
     2101 agtataagtg ttatgaaaaa aacaagccaa aaatgatttg acttattttt ttgaacgagt
     2161 ttttttgaag ctttaaacat aagaggagct ttcttttaat ttgtattttc tttgatagcc
     2221 tttgtaaatg attttaattg agcttctaaa gatagagggt cagagaaata aaacacgtcg
     2281 tagttacttt ctatgatgtg cccttttttg acagctggta aattcttcca gacatcactt
     2341 tctttaagtg atgaagctgc ttttttagtc gttttgttta tattaacaag ggcataatct
     2401 ccaacgtaat caccgattgc ttcttgcgaa acggtaaacc accctttttt aaagacatct
     2461 tttttgactt tttctggggc agcataacct agtgaatcat agattagttc tccaccgcgt
     2521 ccaaaattat taccatataa atagatattt ttatcataaa aatccataat agtaaaagta
     2581 gtgttaggct ttaagatatg gtgtaaatct tttttgacag cgagagtttt agttttccat
     2641 tggctaaccc actgattagc ttctttttct ttaccgaata ctttccccaa ggctggcatc
     2701 atatctaaat aattttgtgc accatattta ataactaaag ttggtgcaat ttttttcaga
     2761 gtattgatgt ttggatcttg atcgaaaacc atgattaaat caggtttttg tgcggcaata
     2821 gcttctgtat catcagcagt taatttttta gcttctttca gttgtttacc aaaaacgggg
     2881 ctatcttttt ctaagtctaa actgtaactt gaaacattaa cacctagttt taataaatac
     2941 ccagtgtaag aatatgtaaa attaattact tttttcggat tttcaggaat ttttccataa
     3001 taggtgaagc cttcaatttt aggcatttta gaaatagttg ttttagtgct ttcttgttta
     3061 gtttgttgtc cgcaagatac caaaaagaag gtcagtagtg tgaggacaat aattccaatt
     3121 tttttcataa gataacatct cctaaataat attaattttg atcagttgat aattgataag
     3181 ataacaaaac aggttgttta ctaatagggt cctgaatgat atgagcatca atcttaaaaa
     3241 tatctttgat aatatcttta gtcatgatct gactaggact accttcatag ataattttac
     3301 cggttttcat tgcaactaga taatcagaat agcgcgcaga caggtttagg tcgtgtagga
     3361 ccataataat tgttttttgc gtttcgtcat taagtttttt gagcaattct aagacttcta
     3421 attgatgatt catgtcaaga taagttgtag gttcgtctaa aaagatagta tctgtatctt
     3481 gtgctaaagc catagcaatc catacttttt gcctctgacc tcctgaaagg tcatcaacat
     3541 ctctattagc aaattgcgct acgttagtca tttccatcgc ccaatggatt ttactgcgat
     3601 cgtcatttgt caaattgcca aggtattttt ggtgtgggaa ccgtccataa gatattagtt
     3661 cataaactgt tatcccttca gttgcttcta acacctgtgg gagaagtgca atcttcttag
     3721 caatttcctt tgtgggtaaa tgtgcaatat catgaccatc aatagtgata gtaccatctt
     3781 ttattttatg gatgcgtgtg agagccttca aaagagtaga ctttccacat ccattggcac
     3841 caataatcgt tgttattttt tggttaagta tactgaggga aagattattg atgatttctt
     3901 tctgatcgta agatacgata atgttttctg cttttatgtg agacatagag aacctttcta
     3961 gcaaaaaatt aagttcatta taacatagaa aaaagactta caaaagcctt ttttagcatt
     4021 aattaagaaa aatgtgaaca aaatgataat tatttggaat gattcaaaat agtatcaata
     4081 atcattattg tttagctttt tttatcgttt aaggtatttt ttcgcaactt ctaaatctcc
     4141 agagtaattg tcacgatttc ctttgataat ttgataagca tctgctcctt taccagcgat
     4201 aataattgca tctagtttgc aattagtaag agtactggca ttagcaattg cttcttctcg
     4261 atcaatgatg atagtaaccg gacgattaat atggcttgct atttcttgtg agataactaa
     4321 gggatcttca aaattaggat catctgcagt gagtatcact tgtagattag gatgttgatt
     4381 aatgacatct ccaaagtctt tacgacggct ttgaccttta ttaccaggag ctccgagtac
     4441 taatataata tccccttttt ggtgttcctc aacaacagct aataatttct tcaaactgtc
     4501 tccgttgtgg gcatagtcaa cgaaaatctt tgcgccgttg gtttgtgtta ggacttccat
     4561 gcggccaggg acggtagttt gtgcaattcc gttttttata tcttcaattg aaacaccaag
     4621 tcggagacaa gcaagtccag ctgcgatagc attttcttga ttgaattttc caatcagctt
     4681 gatatcgtag gtgttctcca actttccttt gacatggaaa gaaaaggcct ttgattctgt
     4741 gataacattt tcagaataat ctccatagaa atcatgagga atatattcga cctgttcttt
     4801 gacaatatta aaatgatcca tctgactatt tacaacaaca gcattgctat tctccatcaa
     4861 gagccgtttg tggaaaaaat aatcttcgaa tgtagggtgc tcgataggac caatatgatc
     4921 gggactaata tttaagaaaa cgccaacgtc aaaggttaaa ccataaacac gtttggttag
     4981 gtatgcttgg cttgatactt ccataattaa gtgtgtcatt tgattctcaa cggctgtagc
     5041 catcatccga aataaatcaa gactctcggg ggttgtgaga tgtgatttaa agaaagattt
     5101 accatctaat gttgtgttca tagttgagag catagcagga cgatggttta cttttaacat
     5161 atgataagca aagtaagccg cagttgtttt tccttttgtt ccagtaaaag ctagaagttt
     5221 aagtttgttt tgtgggttat tatagaaaga catagatatc aaactcatgg cttttttgat
     5281 atcattgact aatatcactg gaatatcggc gccgtaatct atctcagaaa cgtaaaaaga
     5341 aaggcctgct gtgattgcag aatctagata ttctttttta aaagtagcac ccttagcgaa
     5401 aaataaagta tcagatgaaa tttgacgact atcatatgat aaggcattaa aagttacatt
     5461 ttgtgtccag ttataataat aatgctcatg aaacaaaatc tctctgaaat tatggtcatt
     5521 ttttaaaatt tctaaaattt tatctattgt aatcatactc cttattttag actttatgtt
     5581 ctcgttttac aagtctaagg taaaagttta taataaagtg atatttctga gatttttgtc
     5641 ttttaaatgc taaataatat tgaaaataga aagtttacca tgtcacaaaa aacaacaaaa
     5701 gttagtcagc aagaacaaat ggtcaaaggg acggcttggt taacagcagg gaatttcatt
     5761 agtcgcttgc tgggagccat ctatattatc ccttggtatg cttggatggg aaagcatgct
     5821 gccgaagcaa atgctttatt tgggatggga tatgaaatct atgctttatt tcttttgata
     5881 tcaactgttg gaattccagt agcagttgca aaacaggtat ctaagtataa cactttaggt
     5941 aaagaagaga tgagcattta tctggttcgg aaaatcttgc agtttatgct gattttaggt
     6001 ggaatttttg cattaattat gtatattggt tcacctctat ttgcttcact tagtaaagga
     6061 gggcaagaac tcgttccaat attgcgtagt ctaactctag ctgttttagt ttttccttca
     6121 atgagtgttt tacgtggttt ttttcaaggt tttaataatt taaaaccgta tgccattagt
     6181 caagtcgctg agcagattat acgtgtcata tggatgttat taactgcttt ctatattatg
     6241 cgtctaggat caggagatta tattgccgct gtgacacagt caacattcgc cgcctttgtc
     6301 ggtatgtttg ctagtatagc tgtgctgctt tatttcttat ggcgttataa tatgctatct
     6361 gccttgattg gtaaaactcc aaagcacatt aaattagata ctaaagaaat cctaattgag
     6421 actattaagg aagcaatccc ttttattatt actggtgcag caattcaaat tttcaaactg
     6481 attgatcagt tttcatttgg gaatacgatg gcgctcttta cgaattattc aagtgaagaa
     6541 ttacgggtta tgtttgctta cttttcaagc aaccctggta aagttacgat gattctaatt
     6601 gctgttgcta ccgcaattgc tggagttggt attccattgt taacagagaa ttttgttaag
     6661 aatgataaaa aagccgccgc tcgtttggtt gttaataatc tacaaatgtt actaatgttt
     6721 ttactgccag ctgttgcagg atccgttatt ttagcaaaac ctttgtatac agtcttttat
     6781 gggttaccgc agggacaagc tttaggactt tttgtcatat ctcttattca aacaattatt
     6841 ttatctattt atactgtttt agcaccaatg ctacaagcat tgtttgaaaa tcgtaaagct
     6901 attatttact tcctatatgg tctggtggca aaagttattt tacaacttcc ctccattttc
     6961 cttttccatg cctatggacc acttttctca acgacagtag ctctttgtat cccagtaatt
     7021 cttatgtatt tgaaaataca tgaaattaca ggatttaaac gccaagctat tcgtcgtaca
     7081 agtgctttag tccttattct aacgctattg atgagcttta ttatttctat gataatatgg
     7141 ttaatgaatc tggttattgt acccgatagt agactggtaa gtctagttta cattatcgtt
     7201 atcggtgcaa taggtttagg ggtatatggc tttatggcac tggccaccca tctacttgat
     7261 aaaatgattg gtagtcgtgc tcaagactta cgccgaaagt tgcaccttaa ctaatgaaaa
     7321 actaggttag acagaattgt ttaacctagt tttttgacga ccaaaattat ttgtgaaatt
     7381 ttaaggtagt cacaatatag tgagcttatt tttatgttat aattaaatgg agaaatatga
     7441 ggtgaaagat gtcttacgaa aagcttttag aacgattttt aacatacgtc aaaataaata
     7501 caagaagtaa tcctaatagt acacaaacgc caacaactca aagtcaagtt gactttgctt
     7561 taacagtttt aaaaccagaa atggaagcaa ttggtttaaa agatgttcat tatttacctt
     7621 ctaatgggta tttggttgga accttacctg ctacaagcga ccgcttacgc cataaaatag
     7681 gttttatatc ccatatggat acagctgatt tcaatgctga aaatattact ccacaaattg
     7741 ttgactataa aggtggagat attgaacttg gagactcagg ttacatttta agtccaaaag
     7801 attttccaaa tttaaataat taccatgggc aaacactgat tacaacagat ggtaaaacct
     7861 tactgggagc agacgataag tctggtatag cagaaatcat gacagctatg gaatatttgg
     7921 cttcgcatcc agaaattgag cattgtgaaa ttagagttgg ctttggacca gacgaagaaa
     7981 ttggtatagg tgcagataaa tttgatgtta aagattttga tgttgatttt gcctatacag
     8041 tggatggtgg accactagga gaattacagt atgaaacctt tagtgcagct ggtttggagc
     8101 ttacatttga aggacgaaac gttcaccctg gaactgcaaa aaatcaaatg attaatgctt
     8161 tacagcttgc tatggatttt catagtcaat taccagaaaa tgaacgtcct gaacaaacag
     8221 atggctatca aggattttat cacttatatg atttaagtgg aacagttgat caagctaaaa
     8281 gttcatatat cattcgagat tttgaggaag ttgatttctt aaagcgtaag cacttggctc
     8341 aagatatcgc tgataatatg aatgaagcat tacaatctga acgtgtaaag gttaaactat
     8401 acgatcaata ttacaacatg aagaaagtta ttgaaaaaga catgacacct atcaacattg
     8461 ctaaagaagt aatggaagag ttagacatca agccaatcat agaaccgatt cgtggtggta
     8521 cagatggctc taaaatttcc tttatgggaa tccctactcc taatcttttt gcaggtggtg
     8581 aaaacatgca tggacgcttt gaattcgtta gtctacaaac aatggaaaaa gcagttgatg
     8641 ttattttagg catcgttgct aaggattagt aaagaaatat cagattggag tggttaaatg
     8701 ataaaattat ttgggaagat acgttatcac tggcaacctg aactttcttg ggctattatt
     8761 tattggtcaa tagccattgc accaattttt attggtttat ctctgctata tgaaagaact
     8821 gagattccga gtcaggtatt tgttctattt gccatcttta ttgttttagt gggcattgga
     8881 tttcatcgct attttgttat tgaagaagac ggatatctta ggattgtatc ttttaacttt
     8941 ttaagacgaa ctaagtttcc aattgaagat attgctaaaa ttgaggtcac taaatcaagt
     9001 gttactatca aatttaataa taaccatgag cgtatttttt acatgcgaaa atggcctaaa
     9061 aaatattttt tagatgcact agctattgaa cctacattta agggagaagt ggagcttctc
     9121 gataatttga ttaaaatgga ttattttgaa tgttatcgct acgataaaaa agctcttaca
     9181 aaatcgtaag agctttggtg ggacatgatt taactgctaa aacagtatct tgatcagaac
     9241 ttggaatttc tttttctagg ttatctgtat cggaaaattt aacgatacca tcatcttgat
     9301 aatcaaaaat atttgaatat gtttggcaaa gcccgcaggc aatacatttt tctggaatta
     9361 gtgcaacttt catagtatta ttgtaatact tattgatacg attaacaagg gggaagtcat
     9421 ggcgaaaaaa ccatgggaaa aaaaagttgt ggaaaacaat agtcatagaa aagacaaaat
     9481 tacaagaacg agtagaggtg tggtgtcaag tactccttgg attaccgctt tcttgagtgc
     9541 attctttgtt attgtcgttg ctattttatt tatagtattt tatacttcaa atagaggaga
     9601 agacagagca aaagaaactt ctggttttta tggtgcttca agccaaaaag ttaatagttc
     9661 taaaacgaaa aaagcatcta caagtaagaa aacaacgtca tcatcaaaaa ctaaaactag
     9721 tgataaatca acaacatctt ctagtactgt agcaagttcg agtgagacat cgacatcatc
     9781 ttcagtaaac ggtggaacaa tcgtagttca accaggtgag ggagcagcct ccatagctgc
     9841 tcgtgcaggt atctcagttg ctcgtttaca agaacttaat cccagtcata tgacctcagg
     9901 gtattggtat gctaatccag gcgatcctgt ggttgtaaat taaggggagg attatgaact
     9961 caattaatat tgctattgat ggaccagcat caagtggaaa aagtactgtc gctaagatta
    10021 ttgctaaaaa tttgaattac acttaccttg atacaggtgc tatgtatcgt tgtgctactt
    10081 atctggcttt acagcatgga tatgaagcac aagatgtttc gaaaattcta ggactcctag
    10141 ctgaaagacc aatttctttt ggtaaagcag aagatggcag ccagacagtc tttcttggca
    10201 cagaagaagt tactttagct atccgtcaaa acgatgttac taacaatgtc tcgtgggttt
    10261 ctgctatccc agagattcga gaagagttag taaaccagca gcgtcgtata gccaaagatg
    10321 gtgctattat aatggatggt cgtgacatag gaacagttgt tttgccagat gcagaattga
    10381 agattttttt agtggcttct gttgatgaaa gagctgagcg tcgctttaaa gaaaatcaag
    10441 aaaaaggcat tgagagtgat tttgaaacac tgaaatcaga aattgctgct cgtgattata
    10501 aagatagcca tcgagaagtt tcaccattaa aggcagcaga agatgctatt gaatttgata
    10561 ctacaggagt atcaattgaa ggtgttgtaa cttttattca agaaaaagca gaaaaaataa
    10621 ttgacatgaa gaattagtca tgatatacta gttgcaatga gaaaagcaga agtgagaact
    10681 tctcgccttg cgacttaggt tgtctggccc aacatgtcaa gattccatct aggaagatta
    10741 tatgtgcggg cttgatttat caagtccgct ttcgtttttc tctaaaaaaa taaagaggtg
    10801 aagatcatag ctaaaaaaga tctattcatt aacgatgaaa ttcgcgttcg tgaagttcgt
    10861 ctggttggtc tcgagggtga acaattaggt attaaaccat tgtctgaagc tcaagcaatt
    10921 gcagatgatg ctaatgttga tttagtttta attcagccac aagctacacc acctgttgcg
    10981 aaaattatgg actatggtaa gttcaaattt gagtatcaaa agaaacaaaa agaacaacgt
    11041 aaaaaacaaa gcgttgttac tgttaaggaa gttcgtctta gcccagttat tgataaaggg
    11101 gattttgaaa caaaacttcg taacggtcgt aagttccttg aaaaaggtaa caaagttaaa
    11161 gtttctatcc gattcaaagg acgtatgatt actcataaag aaattggagc aaaggtattg
    11221 gcggaatttg ctgaagcgac acaagatatc gctattattg agcaaagagc taagatggat
    11281 ggtcgccaaa tgtttatgca gcttgcacca attccagaca agaaataatt gtcgacttta
    11341 tagtattatt taagaggaga atttaaaatg ccaaaacaaa aaacacaccg cgcatcagct
    11401 aaacgcttta aacgcacagg ttcaggtgga ttgaaacgct tccgcgcgtt tacttcacac
    11461 cgtttccacg gtaaaactaa aaaacaacgt cgtcatcttc gtaaagctac tatggtttca
    11521 tcaggagatt tcaaacgtat taaagcaatg cttactcgcc ttaaataata aggtctctgt
    11581 aaacatttac tacatattat aaaggattat ttggaggaaa tataaatggc tcgtgttaaa
    11641 ggtggagttg tttcacgtaa acgtcgtaaa cgcgtactta aattagctaa aggttactat
    11701 ggtgcaaaac atatcttgtt ccgtactgca aaagaacaag taatgaattc ttactactat
    11761 gcataccgtg accgtcgtca gaaaaaacgt gacttccgta aattatggat cactcgtatc
    11821 aatgcggctg ctcgtatgaa tggtttatca tattcacaat tgatgcacgg tttgaaatta
    11881 gctgaaatcg aagttaaccg taaaatgctt gctgatttag cagttaacga tgcagcagct
    11941 ttcacagctc ttgcagatgc agctaaagct aaacttggta aataagaatt aaaaaagttg
    12001 ggtttgccta acttttttta attactagaa agaaagtacc tatattataa ttttagtgaa
    12061 aagtttcgtt ctatatatgt agaatgaaac ttttttgttt tatttcgata tatttattat
    12121 tgttataatt ttcctttttg atttttattg ttttatagta ttattccgta taaaaagcac
    12181 ctcaatcgtt ggattgaggt gtggattaaa ctttaaatgg caaggattat tttttagatg
    12241 ttttatgtgt cttattagga tcttgcaatt ccaagtaact tggagcatgg aagagatcaa
    12301 ctgttgattt gttgttatgt ttgctgtata tgcttgttga acgatctttc aatttacgtt
    12361 ctttagcttt gagtgcttta agagaatgtg tgtaattgaa tttttcaact tctacagttt
    12421 tcaaaccatt gttttcatca aatcgtagta aatctccagt ttgaatcgaa tcacttgtag
    12481 ataattgttt attagtagca tctcgaatag cttttatttc agctttagtt gtttcatcag
    12541 gatttgtgat ttcttgtcct gaatcagtgt aatagagatg tccactatag ttagtgtatt
    12601 taggagtaat atactgacca gttgttctca tagcgaccat ttgtttatta tctttagata
    12661 ataagtcttg tcctaactga gtatatttag acgtatctat tcctagaata tgaagtaaag
    12721 ttggtaaatt atctacctca ccaccgtatg tattactgat aaaacctttt gaatagccag
    12781 gaatgtgaat catatatgga acacgttgta acatagcgtt gtcgtattca gaccatgttt
    12841 cagggtcttt acctaataat tcagctaagc taggattgcg tgtattagaa ataccgtagt
    12901 gatccccata cattacaata atagaattat catacacacc agcagctttt agatattcaa
    12961 aaaatgattt aagagctgta tcaaggtagt ttgctgtggc aaaataaccg ttaatcgttt
    13021 catcgttagt ctttgcaagt gggaatccct cttcgtcaga ctctcccttc aaacttgtgt
    13081 aagggtaatg gttacttact gtaataaatt tagtataaaa tggttgttgc atgtgctcta
    13141 gatatttgat agagtcagca aacatatatt tatcattcaa accgtattgg aatgaattat
    13201 ctttggtttg ttttgagaag tagctagagt caaagaagta gtcataaccc cactgtttgt
    13261 aagcattatt tctattccaa aatgttccga cgtttccgtg gaagaccgca ctactgtaac
    13321 ctccattttg agctaagata tgtggagcag caaattgtgt attttcacca ccataattaa
    13381 ccatgaatga accactactt agaccaaaaa gtgagttttc catgagtgtc tcagcatcgg
    13441 atgtcttacc agctttgact tgatggaaga agttagagaa ggagacagtc tctttcgaat
    13501 gataaaggct gttaataaat ggagtaacca catgttcttt accatcaata tttaatttat
    13561 agtcaatcaa gaattgttga aaactctcca aatgaatcat gataacgttt cgcccttttc
    13621 cgataccata atattctgga ttgggtttgg cataatgctc tgctacatat tttttggcag
    13681 tcgctaattc ttgtgcagtg gcaccatttc tttctttctg tgcttgataa gtttgattac
    13741 cactataaat cgaaaatgat ggcaagccaa gagctttaac aatataggtg tttgaaaaac
    13801 cacgacttag tagttctgga cgatcaattt cagctaagaa taagttaatt gaaaagagta
    13861 aaccagacaa agctgtgata gaaaaactag cacgtttatt aaatggacga tcatctaagt
    13921 ggattttctt ggttgcaaat aagaaaataa ggataatgaa atcgaaaacg tagacaagat
    13981 cccaaattct caatagatta agagcagagt ctccaaggcc agccgaagtt ttactactag
    14041 ccaagacggc gctaactgta atgaaatcgg aaaactctct atagtagatt gcgtttgcaa
    14101 ttagaaggat atttacgata gcataggtga taaaagcggt aatataaaaa gctttggttc
    14161 gcttaacata taaggccaaa cctatgatta agagtcctaa ggggatgggg tttataatgg
    14221 tcaaaaacaa ctgataggaa ttttctaaac ctaaattaaa atcagtatgg taagcccaaa
    14281 ttgtctttaa ccaatatata actactaaca ataaaataaa gccgagtcgt gtattgatcg
    14341 cacggctagc aaaatcttta atttttttca cagtaggaac ttcctttaat ttttttccta
    14401 aagataaaat ctcagattaa attataacat aaaattttgt aacatacggt aaagaaaggt
    14461 cgaattcagt atgaaaaaag ataaaaattt gttataatac tgtttatgaa taaacttaaa
    14521 gtgaattctg tcgttgaaag aaaaatcaaa tcaggtgctc agttactgga aaaaaaagat
    14581 tttgatacca gtttagttaa ccagttggtt caactttttt cacagtcaaa tcaattctta
    14641 gggatggcct atctttcacc acaaaataaa ggtattggtt ggttactatc aagacaagtt
    14701 tttgatttta accatgatta ctttgtatcg ctattcgaaa aatctagaga gaagcgtcaa
    14761 aaatttgaaa aatctagcca aacaacagcc taccgcttgt ttaatcagga tggagataac
    14821 tttggtggcc taactataga tttttatagt gactatgctc ttttttcgtg gtacaatgaa
    14881 tttgtttata ctaatcgaca aatgattgtt gcagccttta agcaggtcta tcctaatatt
    14941 aaaggggcat atgaaaaaat tcgtttcaaa ggtttagact ttgaaagtgc tcatttgtac
    15001 ggtcaagagg ctcctgaatc atttttgatt ttagaaaata atatcaaata tagtgtcttt
    15061 ttgaatgatg gattaatgac aggtattttc cttgaccaac atgatgtcag aaaagcctta
    15121 gcaactaatc tatcagaagg taaaaaagtt ctaaatatgt tttcatacac tgcgactttt
    15181 tctgtagctg cagctgttgg aggagcatta gagacaactt ctgttgattt agcaaaacgc
    15241 tcgcgtgaac tttcaaaagc acactttgat gctaatcaga ttgtcacaga taaccatcga
    15301 tttatcgtta tggatgtttt tgaatattat aagtatgcca aaagaaaaca tctatcatat
    15361 gatgtgattg ttattgatcc accaagtttt gctcgaaata aaaaacaaac tttttcggtt
    15421 actaaagatt actataaatt aattgaacaa gctttagata ttttaacccc tggtggaact
    15481 atcattgcat caactaacgc agctaatcta accgtatcac aatttaagaa acaattggaa
    15541 aaggggtttg gtaaagcttc acataattac attagcttac agcagttgcc tgaagatttc
    15601 actataaatg ataaggacca acaaagtaat tatttaaaag tatttacaat aaaggtaaaa
    15661 taatgaaaat agtagtacca gtaatgcctc gcagtcttga agaggctcaa gaaatagatt
    15721 tatcaaaatt tgatagtgtt gatattattg aatggcgagc tgatgcctta ccaaaggatg
    15781 acattattaa tgtagctcca gctatttttg agaaattcgc aggtcatgaa attattttta
    15841 ctcttcgtac aacgcgtgaa ggtggtaata ttgtcttatc tgatgctgag tatgttgagt
    15901 taatccagaa aattaattct atctacaatc cagattatat tgattttgag tatttttcac
    15961 ataaagaagt ttttcaagaa atgctagaat ttccaaattt agtcctgtct tatcacaatt
    16021 ttcaagagac accggagaat attatggaga tattttcaga attaacagcc ctagcaccac
    16081 gagttgtgaa aatcgcagta atgccaaaga atgaacaaga tgtcttagac gttatgaatt
    16141 acactcgcgg tttcaagact attaatcctg atcaagttta tgcgacggta tctatgagta
    16201 aaattggacg tatttctcgt tttgctggtg atgtaactgg atctagttgg acatttgcat
    16261 atttagattc atctatcgca cccggacaaa ttactatttc agagatgaag cgtgtcaaag
    16321 cattgcttga cgctgactga tatgagataa caaaatgtta aatatactga tgagatccct
    16381 agcaattgct tagggttttt gtcttattaa aacaaacatg agaggacatt agaatgcagg
    16441 ttgaagttga cttaccgaat cacccttatc atattaaaat tgaagaagga tgtttttctg
    16501 aggctggtga ctgggtatca catttatggc agaaacaaat gataactatt atcacagata
    16561 gtaacgtaga aatattatac ggggagtcgc ttgtcaacca gttaaaaaaa caaggtttta
    16621 cagtacatgt ttttagtttt gctgcgggtg aagctagtaa aactttggaa gttgctaatc
    16681 gtatttatgc ttttttagca aagcatcata tgaccagaag cgatgggatc attgcacttg
    16741 gtggaggcgt cgtcggagat ttagcagcgt ttgttgcttc tacttatatg cggggaatcc
    16801 attttcttca aattccaact agtttaacag cacaggttga ttcttccatt ggcggaaaaa
    16861 caggagtcaa cacttcattc gctaaaaata tggtaggtac ttttgcacaa cctgatggtg
    16921 tactgataga tcctgttact ctaaaaacat tgggcaatcg tgaattagtt gaaggtatgg
    16981 gagaagtcat caaatatggc ttgattgatg atataaaact ctggcatatt ttagaagaga
    17041 tggatgggac tattgatagt atccttgata atgcattagc tattatttat cactcttgtc
    17101 aagttaaacg aaagcatgtt ttagctgatc aatatgataa agggttaagg atgcatttga
    17161 attttggcca cactatcggt catgctatcg aagtacatgc tggctatgga gaaataatgc
    17221 atggagaagc tgttgcaatt ggaatgatcc agctttctcg tgtggctgag cgtaaaaatc
    17281 ttatgccaag aggtatcagt caagatattt ataatatgtg tttaaaattt ggccttccgg
    17341 tccactatgc ggaatgggat aaggacgtct tatttgacat attgagtcat gataaaaaag
    17401 caagtggaca atttattaaa attgttatac tgcctcaatt aggtagtgca acagtccacc
    17461 aaataccttt agaagaaatg agagattatt tggagaaata gatgagatat ttaactgctg
    17521 gtgagtcaca tggacctagt ttaacagcta ttatcgaagg gattcctgcg ggactgaaat
    17581 tatctgctaa agatattaat gaagatttaa aacgccgcca aggtggttat ggtcgtggga
    17641 atcgaatgaa aattgaaact gatcaagtga tcattagttc tggtgtgcgc catggaaaaa
    17701 ccttgggttc acctattaca ctgacagtaa ctaataagga tcatagcaaa tggctggaca
    17761 tcatgtcagt cgaagacatt gaagagcgcc taaagcaaaa acgtcgtatt aagcatccta
    17821 gacctgggca cgcagattta gtaggtggaa ttaaataccg tttcgatgat ttgagaaatg
    17881 ctttagagcg ttcttcggct cgtgagacca caatgcgagt agctattggt gcgattgcta
    17941 aacgtatctt gaaagaaatt ggtatcgaaa ttgccaacca tattgtagtt tttggtggca
    18001 aggagattac tgtccctgat aagctaacag ttcaacaaat caaagtgtta tctagtcaat
    18061 cgcaagtagc tatcgtcaat ccatccttcg aacaagaaat caaggattat attgacagtg
    18121 tgaaaaaggc tggagatact atcggaggag tggttgaaac tatcgttgga ggagttcccg
    18181 ttggtttagg ctcttatgta cactgggata gaaagttaga tgctaagata gcgcaagcag
    18241 ttgtatctat caatgctttt aaaggtgttg aatttggtct aggttttaag tcagggtttt
    18301 taaaaggaag tcaagtaatg gactccatat catggacaaa ggaccaaggt tatatacgtc
    18361 agtcaaataa tttaggcggt tttgaaggcg gtatgactaa tggagagcct attatcgtta
    18421 ggggagtaat gaaaccaatt cctacgcttt acaagccact aatgtcagtt gatattgata
    18481 ctcacgagcc ttatagagct acagtggaac gttctgatcc cacggcactt ccggctgcag
    18541 gtgtcgttat ggaagctgta gtagccaccg tcctggtgac agaagtgcta gaaaaatttt
    18601 cttcagacaa catgtatgaa ctaaaagaag ctgtaaaact atatcgcaat tatgttgatc
    18661 atttttaaat gaaaactagt agtattcaat tgcaaaaaaa ggtataaaag tctaaaatag
    18721 tggtaagttc aagtagaaaa aggagtaact tatggctaat gtatatgatt tagcaaatga
    18781 attagaacgt gctgttcgtg ctttaccaga ataccaagca gttttaactg caaaatcagc
    18841 tattgaaagt gatgcggatg cacaagtgct ttggcaagac tttttggcta cccaatcaaa
    18901 agttcaagaa atgatgcaat ctggccaaat gccaagtcaa gaagaacaag atgaaatgtc
    18961 taaacttggg gaaaaaattg aatccaatga ccttttaaaa gtttattttg accaacaaca
    19021 acggttgtct gtctatatgt ctgatatcga aaaaattgtc tttgcaccca tgcaggactt
    19081 gatgtaatat tataaaagag agagctcctt gagttttctc ttttataata tttcaattta
    19141 tgatagtcta ctgaactagt attgcccaca ttatattggt tttgcattct acgtagcaga
    19201 aaattgtgac tgtaaacact ttgtgataga gtataggtat caatgattca aggagagagt
    19261 aatgtcaaaa caatatgatt atatcgttat tggtggaggt agtgcaggca gtggtaccgc
    19321 taatagggca gccatgtatg gagcaaaagt cctgttaatt gaaggtggac aagtaggtgg
    19381 aacttgtgtt aacttaggtt gtgtaccgaa gaaaatcatg tggtatggtg cacaagtttc
    19441 tgagacactc cataagtata gttcaggtta tggttttgaa gtcaataatc ttaattttga
    19501 ttttactact ctaaaagcta atcgcgatgc ttacgtgcag cggtctagac agtcgtatgc
    19561 cgctaatttt gagcgtaatg gagtcgaaaa gattgatgga tttgctcgtt ttattgataa
    19621 ccatactatt gaagtgaatg gtcagcaata taaagctcct cacattacta ttgcaacagg
    19681 tggacaccct ctttaccctg atattattgg aagtgaactt ggtgagactt ctgatgattt
    19741 ttttggatgg gagaccttac cagattctat attgattgtt ggggcgggct atatcgcggc
    19801 ggaacttgct ggagtggtta atgaattagg cgttgaaacc catcttgcat ttagaaaaga
    19861 ccatattcta cgcggatttg atgacatggt aacaagtgag gttatggctg aaatggagaa
    19921 atcaggtatc tctttacatg ctaaccatgt acctaaatct cttaaacgcg atgaaggtgg
    19981 caagttgatt tttgaagctg aaaatgggaa aacgcttgtc gttgatcgtg taatatgggc
    20041 tatcggccgt ggaccaaatg tagacatggg acttgaaaat accgatattg ttttaaatga
    20101 taaaggttat atcaaagcag atgaatttga gaatacttct gtagatggcg tgtatgctat
    20161 tggagatgtt aatgggaaaa ttgccttgac accggtagca attgcagcag gtcgtcgctt
    20221 atcagaaaga ctttttaatc ataaagataa cgaaaaatta gattaccata atgtaccttc
    20281 agttattttt actcaccctg taattgggac ggtaggactt tcagaagcag cagctatcga
    20341 gcaatttgga gaagataata tcaaagtcta tacatcaact tttacctcta tgtatacggc
    20401 tgttaccact aatcgccaag cagttaagat gaagctcata accctaggaa aagaggaaaa
    20461 agttattggg cttcatggtg ttggttatgg tattgatgaa atgattcaag gtttttcagt
    20521 tgctatcaaa atgggggcta ctaaagcaga ctttgatgat actgttgcta ttcacccaac
    20581 tggatctgag gaatttgtta caatgcgcta acgctatcaa agaatacaaa aaaagctagg
    20641 gaacctagct tttttaaggt gttgttgtat tttcaatagt tgaggtatcg gtagtagtat
    20701 tatttgtttg atcattgtta gtactagatg atgtttgatc gttattggca gcagaatttg
    20761 aactgctact cggagtacta ctagcagagg aattttctag tgtttggctg gatgatgccc
    20821 ttgtctcatc tttttcagta gaaacttggt tactagaatt ttgtccactt tctcttataa
    20881 tgacggtttt tgaagtagaa gaatcagtct tactagagtc tttgtgatta ttaatcgcat
    20941 tagtaatagt aatagaggca acaataatac ttaaaacact accgactaat gcaattgttt
    21001 tttgaagtgc agaaaaacct gtttttatat gcttagcggg tgattgagtt gaatcagtat
    21061 tagtcttagt agactttttc tgttgtcttg aatatccttt tgacatgacg ccctccttaa
    21121 atgtacatca actattatat gattttatcc taaaaaaagc taaagaaaaa ataatttaaa
    21181 acatttaaat aagcaaccta ggttattaag ctatgttagt gtaataggta gtgagttttg
    21241 tgataaatta tgagaattat cataaatatt gttttgggat gttcacaaac taatataaat
    21301 agtaggaaaa ttatgctata ataacactga ttgactttag aaggacagat ttatgattta
    21361 ttttgataat tcggcaacta ctattcccta tccagaagtt ttaaggactt atcaagaagt
    21421 agctagtaaa atctatggaa acccctcaag tttacatgag ctaggaacaa catcttctcg
    21481 gattttagag gctagccgta aacaaattgc tagtttactt gagcttaaag caaatgaaat
    21541 tttctttacc tctggtggta cagaggccga taattgggtt attaagggtc ttgcgtttga
    21601 gaaacaacat tttggaaatc atattattgt ttctgatatc gaacacccag ctgttaaaga
    21661 gtctgctaaa tggcttggag agtatgggtt tgagattgat tatgcgccag tagatgataa
    21721 aggatttgta gacgtagaag cactggtcaa attaattaaa ccagaaacga ttcttatttc
    21781 aattatggca ataaacaatg aaattggttc tattcagcca ataaaagcta tttcagactt
    21841 gttgtcagat aaacctacta tatcgtttca tgttgatgct gttcaagcaa taggtaagat
    21901 accgactaaa gattatttga ctgagcgagt tgattttgca tctttttcta gtcataaatt
    21961 tcacggcgtt cgtggggttg gctttctata tattaaagaa ggaaaacgta tttcaccatt
    22021 actaacaggt ggtggacaag aaactgattt acgttcaact actgagaatg tcgcagggat
    22081 tgcagctact gctaaagctt tacgaatggt tatggacaag gaagtagtag ctattcctaa
    22141 aataagtaaa atgaaaacca ttattcatga tgaattagcc aaatatgagg atattacgtt
    22201 attttcgggt aaggaagatt tttcacctaa cattatcacg tttggtatca agggtgtccg
    22261 aggagaagtt cttgtccatg cctttgaggg acatgatatt tttatctcga caactagcgc
    22321 ttgctcttct aaagcaggta aaccagcagg aactcttata gcaatgggaa tttcaactaa
    22381 gttagcccaa actgctgtac gtatcagctt agatgatgat aatgatatgg gacaagtaga
    22441 gcaattttta actattttta aacaagttta tgaaaaaact aagaaagtta gaggataaga
    22501 tgcagtattc agaaattatg attcgttatg gagaactctc tactaagaag aaaaaccgta
    22561 tgcgcttcat caataagtta aaaaataata tggagcatgt actctccatt tatccagatg
    22621 tttcagtaaa aacagatcgt gatagaggac atgtatatct caatggtaca gattatcatg
    22681 aagttgcaga gtccttaaaa gagatttttg gtatccaagc tttttctcca tcttttaaag
    22741 tagaaaaaaa tgttgataca ttggtaaaag ctgtccagga aattatgact tccgtttata
    22801 aagatgggat gacttttaaa attaccgcaa aacgtagtga ccactcattt gaattggata
    22861 gccgtgctct aaatcatact ttaggagatg ctgttttttc agtcttgcca aatattaagg
    22921 ctcagatgaa gcaaccagat atcaatctta aagtcgagat acgagatgag gctgcttata
    22981 tttcatatga ggatattagg ggtgcaggag gattaccagt aggaacatct ggaaaaggga
    23041 tgctgatgtt gtctggtggg attgattctc cggtagcagg ttacctagcg ttaaaacgtg
    23101 gtgtagatat agaagcagtc catttttcaa gtcctcctta tactagccca ggtgcattga
    23161 aaaaagcaca tgatttaaca cgtaaattga caaaatttgg tggtaatatt caatttattg
    23221 aagttccatt cacagaaatt caagaggaaa ttaaggcaaa agctcccgaa gcctacttga
    23281 tgacgttaac acgtaggttt atgatgcgta ttacagatcg tattcgtgag gaccgaaatg
    23341 gtcttgttat tattaacggt gaaagtttag gacaggtggc aagccaaacg ttagaaagta
    23401 tgcaagctat taatgctgtc actgcaacac cgattattcg tcctgtggtc acgatggata
    23461 agctagaaat tattgatatt gctcaaaaaa tagatacttt tgatatttca attcaaccat
    23521 ttgaggattg ctgtacgatt tttgcaccag atcgcccaaa aactaaccct aaaattaaga
    23581 atacagaaca gtatgagaaa cgtatggatg tagaaggtct tgtagagagg gcagttgcag
    23641 ggattatggt aactactatt caacctcaag cagatagtga tgatgttgat gacttgattg
    23701 acgatttatt ataatgaatt tttaaactac agatttactc tgtagttttt tattaaaatg
    23761 attgcgctat cataaggtta taaaaaatgc tataatacga ataaaggagg caagtatggc
    23821 aagaatccct tatcagaaaa cacttatttt ttgtgtagcc gttattattg ctatttttat
    23881 tttggggtta tctaaggatt tagcacaatc taaagggcaa aaggttgcaa ataataatac
    23941 tgtaaaaaca gcacgtgtgg ttgcaaacgg agatattttg ctgcacgatg tactatatgc
    24001 cagtgcgcgc caacccgacg gcacttataa ttttactcct tattttaaag aggtaaaatc
    24061 ttggattgag agcgctgatt tagcgattgg tgattatgaa gggacgatta gttcagaata
    24121 tcctttagcg ggctatcctt tatttaatgc tcctaatgag attgcaacta ctatgaaaga
    24181 aactggatat gatgtcgttg acttagcaca taatcatatt ctggattcac aactggcagg
    24241 tgctatcaat actgtcaaga cttttaatcg tctgggattg gatactattg gggtttattt
    24301 aaaggataga aataaagaag atatcttaat taagcatgta aatggtatta aaattgctat
    24361 tttaggttat tcgtatggct acaatggtat ggaagctaat gtttcaaaga gtgactatga
    24421 aaaacatatg tctgatttag atactaaaaa gattaagcaa gatattaaaa aggctgaaaa
    24481 agaagcagat atcaccattg tcatgccaca gatggggata gaatatcaaa aaaaacctac
    24541 tacagaacaa gtaatgcttt atcatagtat gattaaatgg ggggcagata ttatattcgg
    24601 aggacaccct cacgttgttg aaccgtctga agtgataaaa aaagatggcc agaaaaaatt
    24661 tattatttac tctatgggaa attttatttc aaatcagcgt ttagaaacgg ttgatgatat
    24721 ctggacagaa agaggattat taatggatgt aaccatagag aaaaaaggtc aaaaaacagt
    24781 aattaaaaaa gttaaggcgc atccaacact agtagaagca aaaccaaatg gccgttatgt
    24841 tgaagaaggc tttccagctt atgattatag gacgctagtt ttagaggatt atattgaagg
    24901 tggtaagaaa cgttctacga ttgatcagac tatgaaagat aaagttgata aagcatataa
    24961 ggagatgaat gagttagtta atttgaagtg gtaaagttga taattttatt ttgttttatc
    25021 ttgcataaaa aaagagttta tgataaaatt tgaatgttga ctaaatgcac atcctgtgca
    25081 accgcgcgaa aatcgttaga ttaagtagag caaggctctc acgatttata ggcgagtctt
    25141 cacagaggga aacctcacaa gaaattttta taggaggtgc atcatgagca catacgcaat
    25201 catcaaaact ggtggtaaac aagttaaagt tgaagtagga caagctatct acgttgaaaa
    25261 acttgacgtt gaagcaggag cagaagttac atttaacgaa gttgttcttg ttggtggtga
    25321 aacaactaaa gttggtactc cagttgttga aggagctact gttgttggta ctgttgaaaa
    25381 acaaggcaaa caaaagaaag ttgtttctta caaatacaaa cctaaaaaag gtagccaccg
    25441 taaacaaggt caccgtcaac cttatacaaa agttgttatc aacgcaatca acgcttaatt
    25501 tttcatgatt aaagcaactt ttactcgtaa tcaatcaggc tatctttata gtgctgaaat
    25561 cagcggtcat gctggtagtg gcgagtacgg ctttgatgtg atttgtgcag ctgttagtac
    25621 tttatcaatt aattttatta attcattaga ggctttaaca acctgccaag cgcaattgat
    25681 tatcaatgac gttgaaggtg gttatatgaa gatagattta tcttctatac cacaacataa
    25741 agaggataag gttcaattat tatttgagtc ttaccttctt ggaatgacta atctgtctaa
    25801 agattcttct gaatttgtta gtacagtagt catgacacac taatttaaga gaggacacaa
    25861 cattatgtta aaaatgaatc ttgctaactt gcaacttttc gcccacaaaa aaggtggagg
    25921 ttctacatca aacggacgtg attcacaagc aaaacgtctt ggagctaaag cagctgacgg
    25981 acaaacagta tcaggtggat caattcttta ccgtcaacgc ggtacacaca tctatccagg
    26041 tgctaacgtt ggacgtggtg gggacgatac actttttgct aaagttgaag gtgttgttcg
    26101 tttcgaacgt aaaggacgcg ataaaaaaca agtgtctgtt tacccaatcg caaaataatg
    26161 cctagttaag actttatgac tattcataag gtcttttatt attgtcagag ctttcatttt
    26221 gtaacaaaag acaatatgtt aagagattta tcattaatac tcataaactt tgttgttctt
    26281 ttatagtgaa atagtcctaa ttcatgaaga attttccgca aggatagtta ttttttggta
    26341 taatgaaggg tactaaaatg aggaagtgat aaaatgaata ttcaacaact gcgttatgtt
    26401 gttgctatcg caaatagtgg tacttttcgt gaagcagctg caaaactatt tgttagtcag
    26461 ccaagtttat ctgttgccgt tcgtgatttg gaaacagaat tagggtttca gatttttacg
    26521 cgtacaacta ctggtgcagt gttgaccaat caaggcatga ctttttatga gaatgcttta
    26581 gaagttgtga aaagctttga ttcattcgaa aaacagtttt cgcaatctga agctacagag
    26641 caagaattct caatagcaag tcaacattat gattttctac ctcctttaat cacagcgttt
    26701 tcaaaatgta atgataattt tagctatttc cgtattttcg aatcaacaac aatacgtatc
    26761 ttagatgaag ttgctcaagg gaatagcgaa ataggtatca tttatatcaa tagtcaaaat
    26821 aaaaaaggtc tacttcagcg gttggacaaa ttggggttag agtttgtaga gcttattcct
    26881 tttaaaactc atatttatct tgggaaagac catcccttgg ctagtaaaac atctctgata
    26941 atgacagatt tagaaggatt accaaccgta cgttttacac aggatagaga tgattatcgg
    27001 tattactcgg agaattttgt ggaagtactt gatagttctg ttacctataa tgttactgat
    27061 agagcaacat taaatggtat tttagaaaga acccaagctt atgcgacggg ctcaggattc
    27121 ttggatagcc gcagtgtgaa tggtatcact gttattccat tagaagatca tctagataac
    27181 caaatgattt atatcaaacg aaaagatcgt aatttatctc agatggcttt aaaatttgtt
    27241 gctgttatgg aagagtattt taagggattt aattcatagg agaagtaatg agaaagatta
    27301 ttataccaat aataactatt ttgttgattg ctttagatca actatctaag ttatggattg
    27361 ttaaacatat tgaactaaac caaattaagg aattcattcc aaatatcgtt agtctaactt
    27421 atctgagaaa ttatggtgct gcattttcta ttttgcaaaa tcaacaatgg ctttttacct
    27481 taattacaat ctttgttgtt ggtgttgcta ttatttattt gatgaaacat attaatggaa
    27541 gttattggtt attaatttct ctaaccttaa tcatctctgg tggattagga aactttatag
    27601 atcgcttaag attaggttat gtcgttgata tggtgcattt agattttatt aattttgcta
    27661 tctttaatgt agcagactca tatctgacaa tcggtattat ttgcttaatg attgcacttt
    27721 ggaaagagga aagtaatgga aatcacaatt aaaatagcag gagttaggct agataaagct
    27781 ttagcggata attctgaact gtcgcgtagt caagctaatg aagaaattaa aaaagggatt
    27841 gtgttagtaa atggacaagt aaaaaaagcg aaatatactg ttcaagaagg agatcggatt
    27901 acttttgata ttcctaaaga ggaagtacta gattatcaag ctgaaaatat ccctttagat
    27961 attatctatc aagatgatga cgttgctgtg gttaataagc cgcaagggat ggttgttcac
    28021 ccttctgcag gccacagttc gggcacctta gttaacgcct tgatgtatca tattaaggat
    28081 ttatcatcaa ttaatggcgt tgttcgccca gggatcgttc atcgtattga taaagatact
    28141 tctggtttat tgatggttgc aaaaaatgat agagctcacc aagtgttggc tgaagagtta
    28201 aaagataaaa aatcgcttcg taaatattta gcaattgtgc atggtaattt accaaatgat
    28261 cgaggtgtga ttgaggctcc gattggtaga agcgataaag atcgtaagaa gcaaaccgtg
    28321 actgctaaag gtaagccagc aataactcgt tttcatgttt tagaacgttt tggtgattat
    28381 accttagttg aattgtcatt ggagacaggt cgaacacacc aaattcgggt tcatatggct
    28441 tatattggcc atcctctagc cggtgatcct gtctacggtc cccgtaagac tttagggggt
    28501 aaaggacagt ttttgcatgc acaaacacta ggttttacgc accctagtaa cggagagaat
    28561 ttgatttttt cagtagaggt tccggaaatt tttcagacaa ccttagaaaa gttgagaaaa
    28621 aattaagttt ttcttgaaaa atacttttac catttgctat aatataagca aatcattcct
    28681 ttaaccttat ccagtgagat agttaaggag aaacttcaga ctaaatcaat gcctgagcgg
    28741 ctttgtcatt ctagagagtc tccttttgaa ggggctcttt tatcttagag aggatactgc
    28801 aatgaaaaga aaagaaatta ttgatgatgt gaccatgaaa cgtgctatta cacgtattac
    28861 ctacgaaatt atagaacgta acaaaaattt agataatatt gtcttagcag gcattaaaac
    28921 aagaggtgtt tttttagcca aacgcattca agaacgcttg aagcaattag aaaatttaga
    28981 tattcccgtt ggagaattgg atactaaacc tttccgtgat gatatgaaag ttgaagttga
    29041 caccacaact atgccagttg acatcactga taaagatatt atattaattg atgatgttct
    29101 ctatacaggt cgcactattc gagctgctat tgataatctt gttagtttag ggcgtccgag
    29161 tcgtgtcagt ttagctgtat taattgaccg tgggcatcgt gaactaccta ttcgtgcaga
    29221 ttatgtgggg aaaaatattc caacaagtca atttgaagaa attctagttg aagtgatgga
    29281 acatgatggt tacgatcgtg tgagtattat tgatccgagt taagtagacg actattattt
    29341 acgatgattt tatagaaggg gaagaaccac aatggcaaaa aaattgttaa ttctagagga
    29401 tggtacggtt tttgaaggac tatcctttgg ttcatctctt gatgtaactg gcgaattagt
    29461 tttttgtacg gggaataccg gctatcagga aataataacg aatccttcac ataatggaaa
    29521 aattttagtt tttacctcac cgctaatagg aaattatggt attcatcgca gttactctga
    29581 agccattatt cccacttgtt taggagttgt tgttgcagag tattccagat gtgtaagtag
    29641 cgatactagc aagatgaatc tagatgaatt tttgaaaatg aaaaaggttc ctgcaatgtc
    29701 gggggtcgat acaaggtatt tgatgcaagt tattaaagaa aaaggatttg taaaagctac
    29761 tttagcagaa gcaggtgatg ttttatcaca tttacaggat caattaatag cgacagtatt
    29821 accgacaaat aatgtggaac aagtttcgac aaaaacagct tatccgtcgc ctgctagcgg
    29881 tcgaaatatt gttgtacttg attttggtct taaacattct attttgagag aattgtctaa
    29941 acgtcagtgt gacgtgactg tcattcccta caatacgtct ttagaaggta ttaaaaatct
    30001 atacccagaa ggtatcatat tatctaatgg tccggggaat ccagaaaagc ttcaggaaat
    30061 cttaaacact attaaagaat tacaaaaatc tgttcctatg ttaggtattg gtttggggca
    30121 ccaattaatt gccatggcaa atggtgcaga aattatgaga ctcccagttg ctaagaaggg
    30181 gccaaattat cccatgagag atattgctac gggtcgttta gaaacagtta gtcagtttaa
    30241 tcattttacc gttaaccggc taaatcttcc gcatgattta ctggtaacac acgaaggttt
    30301 gaatgatcaa gagattgttg ctctaaggca ccgctcattt ccagtgatgt ccgtgcaatt
    30361 ttacccagag gctgctccag gccctcatga tgtgacctat ttttttgatg aatttttaga
    30421 aatgattgga tcacgcgctt aaacataagg tattatttaa agtcagtcgg tgagctgatt
    30481 aataaggagg tgcgtatatg ctaaatacaa aggataaaca gcctattttg cttattattt
    30541 tcggacagaa aatgaaagaa tatgacgatt taattagatc aactgttgag aggttatcta
    30601 aaagggatag acctgttagt attcttatct cgaaggagct aaaccatgtg attcaccatt
    30661 atgatgcaca atttgaaaat cttgagacgc ttgtggggta tataaataca catgaagata
    30721 gtttgattta ccccctttgc ttagataaaa atcatgagct atttcaagag tttttagcgt
    30781 ggcagagtca ttattcaagg cagtatatca gcgataattt atcagaaaaa agtgttcagg
    30841 caatagacaa taatcaagac tttgtgcaag tagattgttt agtaatgaga gactctttga
    30901 ataattgttt atatgtctct gatttagaat atattgaatc taataaaaca actggtaagt
    30961 ctctagcgat tgtaccaagt caaactttat ccgatgctgc taggcaaaca attcgagatg
    31021 ttgcatttga tgtgtgtcga aaagcaaaca ttattggagt atgttacttt agtttcttaa
    31081 tagatttgaa cagtttggat tatcatataa ttagtttatc atctggtttg tctcatcaaa
    31141 gcattttatt tgagactatt acgacctatc ctgttttaga gatagcaact aaattgacag
    31201 tgggatatac ttttagtcaa ctgaagcatt cttattaccc aaatacctct gcctttttag
    31261 agccgcaatt agactatgta gctaccgtat cttttagttt tgaaaaagtt gactatatct
    31321 tttttgctcg taatatagag caattatttt taaatttact cgaggctagc agtcatgatc
    31381 attttccatt tctgagtgat attagcgagg aagatttgat gtttgctttg attcagaaaa
    31441 aagaaaatcg tctagcttat cttttggagg cttttagacg tggctttgat ttgtatgatt
    31501 tatcctcagt tactaaaatc aacccttttt acttagataa atgtttacat attgtagagt
    31561 tgtatgaaaa cctgaataaa agccaatata atgtagatat ttataaggaa gctaaaaggt
    31621 atggtttttc ggatgactat attgctagta gttggcaaat ctctttgata gacatgttag
    31681 aatatcgaaa aaaacatagt gtcgcacctg ttctgaagca agtcgaacaa agttctggtg
    31741 ttctaactgg ccatcaaatt cagtattttc gaagctatga ttggcattca gactacattt
    31801 ctagcggttg tcaaaaggct ttgataatgg tagacaaggg ttattcccta gtaaaattaa
    31861 atgagttaat taagcaaatc aaacaaactc atttagaatt attaattgtt accaatcaac
    31921 ctctcctcat agaacaatta aatgatacat ctataatttt tgacactata ggtattgaga
    31981 ccatattaac aattatgggt atagaagagg tggaagaagt gtatttattg ggagaaagtc
    32041 aagcagacat gttttcaaaa atgaaaaatt tttctatctc ttgtcagtct atttagcaat
    32101 tcttaataaa agctgagatc tatatctcag cttttatttg tggtaaaaag taaagtactt
    32161 tcaattttgt aagttctttc tatgagcaaa atagtatact taatgagtaa aaatattatt
    32221 tttaaagaat tttcttgaca atattattta aaaataatac acttgttcta tattaataat
    32281 acaaacagga gatggacatg agtaaacgac aaaatttagg aattagtaaa aaaggagcaa
    32341 ttatatcagg gctctcagtg gcactaattg tagtaatagg tggcttttta tgggtacaat
    32401 ctcaacctaa taagagtgca gtaaaaacta actacaaagt ttttaatgtt agagaaggaa
    32461 gtgtttcgtc ctcaactctt ttgacaggaa aagctaaggc taatcaagaa cagtatgtgt
    32521 attttgatgc taataaaggt aatcgagcaa ctgtcacagt taaagtgggt gataaaatca
    32581 cagctggtca gcagttagtt caatatgata caacaactgc acaagcagcc tacgacactg
    32641 ctaatcgtca attaaataaa gtagcgcgtc agattaataa tctaaagaca acaggaagtc
    32701 ttccagctat ggaatcaagt gatcaatctt cttcatcatc acaaggacaa gggactcaat
    32761 cgactagtgg tgcgacgaat cgtctacagc aaaattatca aagtcaagct aatgcttcat
    32821 acaaccaaca acttcaagat ttgaatgatg cttatgcaga tgcacaggca gaagtaaata
    32881 aagcacaaaa agcattgaat gatactgtta ttacaagtga cgtatcaggg acagttgttg
    32941 aagttaatag tgatattgat ccagcttcaa aaactagtca agtacttgtc catgtagcaa
    33001 ctgaaggtaa actccaagta caaggaacga tgagtgagta tgatttggct aatgttaaaa
    33061 aagaccaggc tgttaaaata aaatctaagg tctatcctga caaggaatgg gaaggtaaaa
    33121 tttcatatat ctcaaattat ccagaagcag aagcaaacaa caatgactct aataacggct
    33181 ctagtgctgt aaattataaa tataaagtag atattactag ccctctcgat gcattaaaac
    33241 aaggttttac cgtatcagtt gaagtagtta atggagataa gcaccttatt gtccctacaa
    33301 gttctgtgat aaacaaagat aataaacact ttgtttgggt atacaatgat tctaatcgta
    33361 aaatttccaa agttgaagtc aaaattggta aagctgatgc taagacacaa gaaattttat
    33421 caggtttgaa agcaggacaa atcgtggtta ctaatccaag taaaaccttc aaggatgggc
    33481 aaaaaattga taatattgaa tcaatcgatc ttaactctaa taagaaatca gaggtgaaat
    33541 aatcgtgaca actaatcgaa aagaactgat tagattgcat cagattgtta aaagttatca
    33601 aaatggggat caaaaacttc aagtcttaaa gaacattgat ttgacggttt atgaaggtga
    33661 atttcttgca atcatggggc catctggttc agggaaatcc acactaatga atattattgg
    33721 cttgttggat tctccaactt caggagatta tagtttaaat ggtaaacgtg tcgaagaact
    33781 aagtcaaaca aaattagctc aagttcgaaa taaggaaatt ggttttgtct ttcaacaatt
    33841 tttcttgtta tctaaactaa cggctttaca aaatgttgaa ttacccttaa tttatgcggg
    33901 tgtacctcct aaaaagcgta aaaatttagc aaaacaattt ttagataaag ttgaattaag
    33961 agagcgtatg aaccatctgc caacagaatt atctggggga cagaagcaac gtgtagcaat
    34021 cgcgcgtgct ttggtgaata gcccatctat tattctagca gatgagccga ctggtgcctt
    34081 ggatacaaag acgggtgagc aaattatgca gttcctaacg gaattaaatc aagaaggtaa
    34141 aaccattatc atggtaactc atgaaccaga aatcgccgat tatgcgactc gtaaaattgt
    34201 tattcgtgat ggcgaaatta cagcagatac tactgatagt attagaattg attaagggga
    34261 gattcgatta tggaaaattg gaagtttgcc ctatcatcga ttctagggca caagatgcgt
    34321 gcttttctga ccatgttagg gatcatcatt ggggttgcat cagttgtgtt aattatggcc
    34381 ttaggtaagg gcatgaaaga ttcggttaca aatgagatta caaagtctca gaagaacctt
    34441 cagatttatt ataaaacaaa agaagaccaa aaaaatgagg ataactttgg agctcaaggg
    34501 gctttcatgc aaggtagtga taccaaccgt aaggaaccaa ttattcaaga atcatggtta
    34561 aaaaagattg ctaaggaagt agatggtgtt agtggctact atgtaaccaa tcaaacaaat
    34621 gctccagtag cttaccttga aaaaaaggca aagacggtta atataacagg aattaaccgc
    34681 acttaccttg gtatcaaaaa gttcaaaatt aaaagtggtc gtcaattcca agaggaggat
    34741 tataaccaat tctcgagagt tattttacta gaagaaaaat tagctcagag attattccag
    34801 acaaatgaag cagcgttaaa taaggtggtg actgtaaaaa ataaatctta cttagtagtg
    34861 ggggtttatt cggatccaga agctggttca ggcttatatg gaagtaattc tgatgggaat
    34921 gctattttaa ccaatacaca gttagcttcg gagtttggtg ctaaagaagc agaaaacata
    34981 tattttcatc ttaatgatgt atcgcaatcc aatcgcattg gtaaagaaat aggcaaacgt
    35041 ttaacagaca ttagtcatgc taaggatggg tactatgata attttgacat gacatctata
    35101 gttaaatcta tcaataccca agttggtatt atgacaggtg ttattggagc gattgcagca
    35161 atttctctcc tagttggtgg tatcggagtg atgaatatta tgttagtctc tgtaactgag
    35221 cgcacgagag aaattggcct tcgtaaagcc cttggggcaa ctcgacgtaa aattttagct
    35281 caatttttaa ttgaatcaat ggtcttaacg attttgggtg gattaattgg gttattacta
    35341 gcctatggag gtacgatgtt aattgctaat gcgcaagata agattactcc ttctgtgtca
    35401 cttaacgtgg ctataggcag tttaattttc tcagcattta ttggaattat atttggttta
    35461 ttacctgcta ataaagccag caaattaaac ccaattgatg ctttacgata tgaataaaaa
    35521 aacatgtact ctgtcaagat tttttcttga cagagtttgt atttatgtta tacttagtaa
    35581 ggtaaagttg ttagttaagc tatcaacgac caaatataaa gaaaaagaaa agagactatt
    35641 taaaaatggc agtaaaaatc cgtttaactc gtatgggttc taagaagaaa cctttctacc
    35701 gtatcaatgt tgcagattca cgtgcaccac gtgacggacg ttttattgaa actgttggaa
    35761 catacaaccc attagttgca gaaaaccaag ttacaatcaa agaagaacgt gttcttgaat
    35821 ggttatcaaa aggtgcacaa ccttcagata cagttcgtaa ccttctttca aaagctggag
    35881 ttatgactaa attccacgat caaaaattct caaaataata aaagcttatg gataccattg
    35941 aaaatctcat tattgctatt gtgaaacctt tgatttcaca accagatcag ttaaccataa
    36001 aaattcagga tggcccagaa tttttagagt accatcttga tttagatacc caagacatcg
    36061 gtcgtgttat cggcaaaaag ggtcgtacaa ttacagcgat aaggtcgatt gtctattcgg
    36121 taccaacaca agggaaaaaa gtaagactca tcattgatga gaaagaataa tttattctaa
    36181 aagataggaa cttcctgtct tttttgttat atctacacaa aaattgtgag ttttttagtc
    36241 ttttagaata taatggatgt gaaagggaat tttctgattt tttattaagg aggataagat
    36301 attgctattt gattttctcg aagaaaaaat aaaactgaaa gcatacctga tgaccgcctt
    36361 acttgatgat ggagaaatta accttgatca ctttaaaaca gaatatgctt tgagtgaaaa
    36421 gattttacaa ggtcttattt cggaactaca aatactttat tctgatttct accttgttgt
    36481 caaaagtaga acaatggtat tgttccatta cgaaacactt tctaagctag agactttgca
    36541 taccatttat cgagagtcta atacgttaca gtttttggca tattttattg ctcctcaaaa
    36601 tgtcagtttt tctgaattca cggctaagaa attcatatcc gtcgcaagtg cctaccgaat
    36661 aaagaaaaac tgtaaacttt atttggagag tgttaagtta tcaatacaaa aaaataaagt
    36721 gacaggtcca gaatatcaga ttaggctgct gattgctttg ttacagtatc attttggttt
    36781 tattatttat ccttttgatg ctacaagtga ggtaatgata agggatttta ttgaatactc
    36841 gaattttaaa gggtgtgact acgaccttaa agacttatct gaagaatatt actattttta
    36901 tattttggtg aatttagttt ggaaaagaaa agaatataaa gtagaaattc ctcaaacaga
    36961 atcctttaat caactaaaaa aatctattct ctacatggag ttatccaaag cagctcaaaa
    37021 gagtattgct atacatcttg gtatagagtt gaatgaaggt ttacttgatt atctatattt
    37081 agtgttttgt attagtgatg gtcctctatt tagcaatcga gatgatatca atatcaacga
    37141 agaatttatt tttgcttctt ataagatatc aaagtctaaa gcctttaaga atttttgtgg
    37201 tcgattattg ggtagagaat ttattgactc gcagcatttt aaagatatta atgcctattt
    37261 tttgagacat tttatttgtt attgttatta ttttattcct gatttttatt ttttaaatac
    37321 atcaaggctg agctattcta aggatttata tcacctatta gataaaggtc tggctgatat
    37381 ttttaacctt aaaggaggga atttaacttt ttcaaaacat gagacagtgc tactaacaat
    37441 gcaattatct aacttaatag agactttctt agctcctctt tctgtatatg taatttcatc
    37501 tagtaatatt agattacaaa cctatcaagt aatgttaaac caatatttta catctaagat
    37561 agcggaattc ttttttgtaa actatcaaac gactcagata gatgaaaaat tactaaaaaa
    37621 agcagatatc attattgctg agagacgcta tatatcttct
//
LOCUS       DATRMA010000011        37285 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.011, whole genome shotgun sequence.
ACCESSION   DATRMA010000011 DATRMA010000000
VERSION     DATRMA010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 37285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37285
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.011"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            62..313
                     /locus_tag="VBC28_000515"
     CDS             62..313
                     /locus_tag="VBC28_000515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6814517.1"
                     /translation="MKKRKRDISSLKERINYFQDVYDYMLVNSETSFIEKDWSDNVRI
                     MSMEDIKEEILVSQNRLEELQNQTFKKNVLKLVHRLFRR"
     gene            complement(267..1055)
                     /locus_tag="VBC28_000516"
     CDS             complement(267..1055)
                     /locus_tag="VBC28_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000092751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6814518.1"
                     /translation="MSRYTDNKHASSILKNIEDRPEKYLIIHYSCEDFNALKGKSPRI
                     ASISIRQFNNAQTNNFSIHQYSEMLNIPITDNTYKDIEKELLTDFFAFIDKNLDKIWI
                     HWNMRDSVFGFNALEQRFKVLGGHPIAIDNDKKIDLGHLFKLLYGGDYIGNPHIVKLM
                     ERNNLTPKYFLNGKDEAKAFDDNEYVKLSMSTSVKANLFFNFLTQALNGTLKTDVPKW
                     KIRGSNIVGLWSTFESTLRGKTIIWIINLLIGGIIGALISKHFS"
     gene            1106..1321
                     /locus_tag="VBC28_000517"
     CDS             1106..1321
                     /locus_tag="VBC28_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015997031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEO6814519.1"
                     /translation="MSQQHRKWAELVEARLKEKGWNKADLTQAIGLRSQGTITDLLKL
                     GKGSADLKIRVSKILGIREPWEKFEEG"
     gene            1433..1744
                     /locus_tag="VBC28_000518"
     CDS             1433..1744
                     /locus_tag="VBC28_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001123482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HEO6814520.1"
                     /translation="MPKAELVYRPAKQSEKADYGDYVHLCQIWEGLTIGTAKVWAAEM
                     RNHPDFKQFINNPTHRIVFINYEGFKLFVTWKSRNRYRPKKETLAEMIENIKREKQLG
                     V"
     gene            1749..1898
                     /locus_tag="VBC28_000519"
     CDS             1749..1898
                     /locus_tag="VBC28_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000732607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6814521.1"
                     /translation="MKKLFKKLFVKKNQVIETRQKWTIETHGWEANARRYDNMIERNN
                     RGRTC"
     gene            1892..2119
                     /locus_tag="VBC28_000520"
     CDS             1892..2119
                     /locus_tag="VBC28_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000910901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6814522.1"
                     /translation="MLKEVLLENELLRDENRRLTNEMADYYFTNVAKANLLDIIVNEG
                     YILDSTLEKCIAKLDNVDRQTLEKNWSDDND"
     gene            2112..2342
                     /locus_tag="VBC28_000521"
     CDS             2112..2342
                     /locus_tag="VBC28_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6814523.1"
                     /translation="MIEVERQPKNTYSRIRCSNNIYDEIANIANECDLTLKEVTDALL
                     NYGLANAQIISSEKVVTESKLVIGDFDNDNNN"
     gene            2326..3645
                     /locus_tag="VBC28_000522"
     CDS             2326..3645
                     /locus_tag="VBC28_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000156423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEO6814524.1"
                     /translation="MTITINKLEIENVKRIKAVKVEPSSKGLTIIGGNNNQGKTSVLD
                     SIAWALGGNKYKPSQAVRDGSQVPPTLKITMSNGLIVERKGKNSSLKVIDPNGQKGGQ
                     QLLDSFVEELAINLPKFMDSTPKEKATILLQIIGVGEQLYQLEQQEKTKYDERTAIGR
                     IADQKKKFADELPSYPDAPKDLVSITDLIQEQQAVLAKNGENARKRQNLEQITTNFNN
                     AELKVAQLQEALAAAIDERDIAKQDLAIAQKDTMDLVDESTAEIEGNIARIDEINRKV
                     RVNLDKEKAEMDAKAHKEQYDLLTSEIEAIRKQITDLLDNADLPLPGLTVADGKLLYQ
                     GQEWDNMSGSQQLIVSTAIVRKLKPDCGFVLVDKLEQLDQPTLEFYGQWLEQEGLQAI
                     ATRVSTGEECAIIIEDGYSVENKANSFKTAEGGSFAETVAPTWQGGF"
     gene            3660..4754
                     /locus_tag="VBC28_000523"
     CDS             3660..4754
                     /locus_tag="VBC28_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001167564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEO6814525.1"
                     /translation="MKITRGKRARAQRVIIYGPEGIGKSSFAANFPEPLFIDTEGSTD
                     NMDVARMDKPTSYTMLKNQIAWIKANPTCCKTLVIDTIDWAESLIIDDVCAQHGKKGI
                     EDFGWGNGYTYTKEEVGRFLNMLQELIELGINVVLTAHAQMRKFEQPDEMGAYDRWEL
                     KLGKKTSSQTAPLVKEWADMVLFANYKTVVMTADNNKKKATGGARVLYTQHHPAWDAK
                     NRHGLQEEMPLDYAGIAHIFAQAPSQPVTETPPVQQTTPAQSVHDPAHEHRTTSQPQQ
                     VPENETGQQVVYPSSLPQALTDLMMAEQVTPDELVAVANIRGHFPPLTPIENFPPDYW
                     NMIVANWGATLEVIKTQVRATEQPFTVEGS"
     gene            4794..5216
                     /locus_tag="VBC28_000524"
     CDS             4794..5216
                     /locus_tag="VBC28_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6814526.1"
                     /translation="MDKTIKLDLSAIGEGGLQEKVDKELEKVFDNILDPNTDIKTKRK
                     LTITLTMTPDETREVVSTSMEVKSTLAPQTGVATTVLVGQKDGKVYANELKSKIPGQT
                     YFDEEATLRTDIGQPIDDLERGINEDIIDFNKQKKVGN"
     gene            5218..5952
                     /locus_tag="VBC28_000525"
     CDS             5218..5952
                     /locus_tag="VBC28_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6814527.1"
                     /translation="MSENIKEALEYAVNIANQMPETILGNDGKEYYDRNKYSLVELKT
                     KYYPKTLNLNTLDSLIDYLKSDMNNINSKRLMVIVEGPREVTVCEEDDDELNRNVLVT
                     VEAIIPNVQFGRYDSPSDFNISLQSLFVNADDRNTVIEFASALKIENGSEIVDDGIGQ
                     TATIKQGVASLAKAKAPNPVTLRPYRTFSEVEQPSSQFIFRINQFANMALFEADGGKW
                     RLDAINNIADYLKTALIDQTNITILA"
     gene            5973..6578
                     /locus_tag="VBC28_000526"
     CDS             5973..6578
                     /locus_tag="VBC28_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000141561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6814528.1"
                     /translation="MTEYNNNFEREFGWDDTIQEDAKEFITLQPGDYVFTVTDFERAR
                     HTPNPQKTGKLPACNKAVISLEIETDEGVASLKHNLFLHSSTEGMLSAFFGAIGQKKH
                     GEPLKMNWNTVVGATGVCSVKNRTYNDNFYNEVKSMIYADSVDWTKVLNANTQPQAPQ
                     PTYQQPAQQTTNFAPQQQPAPQSYQQGQMQAPQQQGGWGGF"
     gene            6578..8161
                     /locus_tag="VBC28_000527"
     CDS             6578..8161
                     /locus_tag="VBC28_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEO6814529.1"
                     /translation="MQLRPYQEEARSAVQQEWEQGKKKTLLVLPTGCGKTIVFSKIIE
                     DRVKLGERVLVLAHRSELLEQASDKLMTATGLGTALEKAENTSLGSWFRVVVGSVQTM
                     QREKRLSKFPTGYFDTIVVDEAHHAISDGYQRVLNHFEESNVLGVTATPDRGDKQNLG
                     KYFDSLAYEYSLVDAIKSGYLSKITAVTIPLTLDLSAVSQQAGDFKASEIGTALDPYL
                     EQIADEMVKQCANRKTVVFLPLVKTSQKFRDILNQKGFKAAEVNGESKDRAEVLEDFD
                     NDKYNVLCNSMLLTEGWDCPTVDCVVVLRPTKVRALYSQMVGRGTRLAPGKENLLLLD
                     FLWHTERHELCRPAHLIAKTPEVAQKMVENMTDETEVAFELLEAEEVASKDVVAEREE
                     ALAKQLAEMRKRKRALVDPLQFEMSINAEDLVDYVPEFGWEMAPASEKQLKTLEKFGI
                     FTDEIGNAGKAAKLLDRLDKRRQSGLTTPKQIRMLERYGFKNVGMWKFEVASNMINRI
                     AASGWRLPKGINAREYQPE"
     gene            8170..8367
                     /locus_tag="VBC28_000528"
     CDS             8170..8367
                     /locus_tag="VBC28_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6814530.1"
                     /translation="MENINEQIEKFISNFADEAIEKSETYSEAILYVDKHSSLTEFGQ
                     VVKKAIQEKIRDIALNSRIIK"
     gene            complement(8360..8611)
                     /locus_tag="VBC28_000529"
     CDS             complement(8360..8611)
                     /locus_tag="VBC28_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6814531.1"
                     /translation="MPEIKITFSDGSIVIFHEEQSFQSVVKSNDAMSLSKIYSLWNHV
                     HDGLIPSFLELIANSQFFFDLENPQIYYASNAVIKIEAI"
     gene            8682..10961
                     /locus_tag="VBC28_000530"
     CDS             8682..10961
                     /locus_tag="VBC28_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEO6814532.1"
                     /translation="MAENDFNLLPLLDYINPATVDYQTWVQVGMALKHEGYTAMDWDV
                     WSQSDSRYKKGECFAKWDSFQGNGFGTITGATITQLAKDNGWTSSEYRNSDDAHELSW
                     DDTIDRDYKIVDKNWIESKEIQEPRNWNPVQDLITYIDTLFESTDKVGYVTETYPITL
                     DTGEIVYKPTKGAYDRTAGQLIESLQKNPTDLGAVFGDFKEEAGAWIRFNPLDGNGVK
                     NDNVTDFRYALVESDTMELGKQYALFKELELPIATLVHSGKKSLHAIVKVDARDYQEY
                     RKRVDYIYQICKKNGLDIDTQNRNPSRLSRMPGIMRNGHKQFLIDTNIGKTNYEEWYQ
                     WIEDLNDDLPEPETLADEWDNLPELAPELIKGVLRQGHKMLIAGPSKAGKSFALIELS
                     IALAEGQKWLGWQCEQGKVLYVNLELDRPSALHRFKDVYDAMGLPPTNVANIDIWNLR
                     GKTVPMDKLAPKLIRRSLKKNYQAVIIDPIYKVLTGDENSADQMAHFTNQFDKVATEL
                     GCSVIYCHHHSKGSQGGKKSMDRASGSGVFARDPDALIDLVELELTDELIKQRVEKAS
                     AKVYQKALQEQNLSYYQHEVTLDDLESRYQMQQHFDKAIKDIMVKQPYLNEIKRITHA
                     IEISTGWRVEGTLREFAKFQPVNMWFSYPVHEVDTTGVLADISLEDNVPTWKKNFEKK
                     SPRESREKKSQKVETAINSLNDGIEPVTIDNLVEYFSTEDKPVSEKTIRRWIKENGKF
                     EVKNKQILPIEEPKTNKSL"
     gene            11355..11729
                     /locus_tag="VBC28_000531"
     CDS             11355..11729
                     /locus_tag="VBC28_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HEO6814533.1"
                     /translation="MKQIPTTTHQQKKVRVVNGKPQFYEPDSLKEVRAMFMSKFAPHA
                     PEKKMTGPIRLTVKWLFPRIKKSIDGQYKDTKPDTDNLNKLPKDCMTELGFWNDDAQV
                     ASEIIEKFWANTVGIYVKVEEL"
     gene            11726..11947
                     /locus_tag="VBC28_000532"
     CDS             11726..11947
                     /locus_tag="VBC28_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6814534.1"
                     /translation="MKIDYIDFFERVVPGWMRESNVKIQEVGFNTEAYWVWANQSIVA
                     ICEKYGNDSLINGQFHLIWEWLEDKANGG"
     gene            11950..12366
                     /locus_tag="VBC28_000533"
     CDS             11950..12366
                     /locus_tag="VBC28_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6814535.1"
                     /translation="MIPKFRAFNKKTKKMYSVDGFKSSERKIYRCSLADDEFRPGHME
                     TFHFVEDNLDDYVLMQSTGEFDENGVEIWEGDIVLTTRLIDYTYKNFKGVVKMLEGCW
                     LIDTGTDAVYLWSEIEENEVIGNIYENPELLESVEE"
     gene            12363..12500
                     /locus_tag="VBC28_000534"
     CDS             12363..12500
                     /locus_tag="VBC28_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6814536.1"
                     /translation="MKLSTFIELLQEDDMKPDWEVTCGIAIDPQKRRIWFPEPHESVD
                     E"
     gene            12497..12712
                     /locus_tag="VBC28_000535"
     CDS             12497..12712
                     /locus_tag="VBC28_000535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein Cse60"
                     /protein_id="HEO6814537.1"
                     /translation="MIKTKIFTTFGTGDNYTCADDQCNKFIANNPKIKPIDFKFQGNV
                     SGYADGGINGSDYYEAILMIYEESVDK"
     gene            12709..13029
                     /locus_tag="VBC28_000536"
     CDS             12709..13029
                     /locus_tag="VBC28_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6814538.1"
                     /translation="MKIDFEDKIKGVYGWTIKDGKVNPPKHNLPKSVKERADYFWEMT
                     EHGLTFLGALDYIFMDEKPEGYDTFATKEWLEKTKEFNEWFHNSPNMAQAEIAIYLLY
                     GNNK"
     gene            13026..13244
                     /locus_tag="VBC28_000537"
     CDS             13026..13244
                     /locus_tag="VBC28_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6814539.1"
                     /translation="MKQAYSFYTQEIICLAVFLLIFGGLLGGFIIQNYCDSYYQPQIE
                     GLQRQLDKSHEQLAKKTKRIAELTGNGG"
     gene            13241..13678
                     /locus_tag="VBC28_000538"
     CDS             13241..13678
                     /locus_tag="VBC28_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017827944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine methyltransferase"
                     /protein_id="HEO6814540.1"
                     /translation="MMVVQKSLLSSDKDYWETPQNFFEKLNEEFNFNLDVASSDNNAK
                     CKNHFTVDDDGLSQDWTGNVFCNPPYGREIGKWVEKAYKESLEPYNNVIVLLIPARTD
                     TKYWHDYIFGKATDIRFLKGRLKFTINGKENHPAPFPSAVIIY"
     gene            13691..14164
                     /locus_tag="VBC28_000539"
     CDS             13691..14164
                     /locus_tag="VBC28_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HEO6814541.1"
                     /translation="MKIEEAKELVDNSNFYGKTISVIKAEVHAIIDQLNPPKPEIPQF
                     VADWYEKHKDSLEYYIWEYINEWDNQEQDDFFNFMNYGTFKPIQTLVNMHQFGYEIEQ
                     KKLYTVDLPNGQPLVSGKNTLYFSQNLAAENVKLTESEIRKDFDWAWQFAEEVTE"
     gene            14161..14574
                     /locus_tag="VBC28_000540"
     CDS             14161..14574
                     /locus_tag="VBC28_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEO6814542.1"
                     /translation="MSRLSNAQLKAFDEWLFDYRDIERKIAIRKLELQTPVSTDINVG
                     GGKSNLVAKETEDIVIKWSKDGKIKSYENFRESVDRVKALLDDELNYIFDLRWGTGSN
                     NTWEEIAYKIHTSRAGTYRKRDRILTLFAQQIGKL"
     gene            14695..15126
                     /locus_tag="VBC28_000541"
     CDS             14695..15126
                     /locus_tag="VBC28_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HEO6814543.1"
                     /translation="MSKLTLKQKRFADEYIISGNTEKSALIAGYSKNYARAQAHKLLA
                     NVGIKSYIDERLEKLESAKIATQEEVLQYLTSIMRGDQQEKTLISIGELGQEIVDIDV
                     GAKDRIKAAELLGKRYRLFTDKVEMDVSSDVTINVGEWDDD"
     gene            15116..16393
                     /locus_tag="VBC28_000542"
     CDS             15116..16393
                     /locus_tag="VBC28_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HEO6814544.1"
                     /translation="MTIKQRPKINIIIKHPSKVFNKHIYDKLYNYSNFTEVHYGGASS
                     GKSHGVFQKIILKALNPKFKHPRKILVLRKVGATVRDSVFADIMSTLSYFGILDKCKI
                     NMSAFRITLPNGSELIFKGMDNPEKIKSIKGISDVVMEEASEFTLDDYTQLTLRLRDK
                     KHLEKQIYLMFNPVSKVNWVYKAFFVKTPKNTVVYQTTYKDNRFLDDVTRENIEELAD
                     RNEAYYKIYALGQFATLDKLIFPKYDKQILNKDKLSHLPSFFGLDYGFINDPSAFLHV
                     KIDDANKKLYILEEYVRKNLTNDKIANAIKDLGYAKEEIRGDSAEKKSNQELRNLGIP
                     RMIDVTKGPGTVMQGIQYLLQYDWIVDERCVKTIEELENYTWKKDKKTNEYINEPVDS
                     YNHCIDAIRYAVQDRIYQSADRSKRMKNAKYYF"
     gene            16409..17941
                     /locus_tag="VBC28_000543"
     CDS             16409..17941
                     /locus_tag="VBC28_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEO6814545.1"
                     /translation="MEEKQFLVGTRFNENANKQFIMLQEDFEAIDYESKLWIDQLKNY
                     ISRFKAEQLERLKELKRYYLGDNNIKYRPAKTDKYAADNRIASDFAKYITVFEQGYML
                     GVPVEYKNENKDLQAAIDLMSVRNNEDYHNVKIKTDLSIYGRAYELLTVEEIDDKKTE
                     VKLYQLPAEQTFVIYDDTYQRNSLMAVHFYDIDYGSGKRKQIIKVYTSDTIYTYEDYN
                     FETKGMRLKEHEEHFFKGVPVNEYANNEERTGAYESVLDNIDAYDLSQSELANFQQDS
                     VNALLVIAGNTYTGADENDYLDDGRLNPNGRLAISIGFKKAQVLILDDNPNPNGVKPQ
                     AYFLKKEYDTAGSEAYKNRLVADILRFTFTPDTQDMKFSGVQSGESMKYKLMASDNYR
                     EKQERLFKKGLMRRLRLAANIWAIKGNEATTYSLVNDTSIVFTPNLPQNDNEIVTAAQ
                     NLYGIVSDQTIFEILNTVTGVDAEAELERLKEEADKKQSLPEPRLVGDASGQEEPTAE
                     KP"
     gene            17901..19349
                     /locus_tag="VBC28_000544"
     CDS             17901..19349
                     /locus_tag="VBC28_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HEO6814546.1"
                     /translation="MLVVKKNQQQKSLNYWQKRQEDILSYLDRTDLDVFSELQKLYNE
                     QAFELQKELFDFYTKYSEENKMTYQDVVKKLRHEDLSDYVANANKYRKQAEKDPELLK
                     RLNEQYVSARATRMDALNLELVYRAGILKGVLDSVFENHLKKAASYAYKKAMGGRSGT
                     INGPVLEELVRTPFDGYNYSEQLWGNTDNLVKNLQKKLKQGFVRGEHPRAIARDLAKQ
                     FNVANHRAETLIRTDGTMVINNATARRYLNAGLKYYRDLVRLDDRTTEICRKIAKENK
                     RKLLSELKPGINAAPYHFNCRTTIIPDEDELSIEVDPIDDKSTKYFKDVTSDWIDGNE
                     HKPQLSLLNEYVKNGTPYKVDGHNVVLDHSNYEYRVANWLSKKTGLQVDMVPRVNSPE
                     HINTPDYLVDGVPFDLKEITGSGKNVIDGNLRKAKKQATNIIFDITKTPLSFEEIMGQ
                     LEHIYMLDRRGLDISIIKNKDEVLAVLEKEGR"
     gene            19377..19565
                     /locus_tag="VBC28_000545"
     CDS             19377..19565
                     /locus_tag="VBC28_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6814547.1"
                     /translation="MGVRPSSFFISIIHQPIKTIKEENMFIWQMVLVALGVLVLILIV
                     GIAAIAVKSIIAELKKVG"
     gene            19568..19834
                     /locus_tag="VBC28_000546"
     CDS             19568..19834
                     /locus_tag="VBC28_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6814548.1"
                     /translation="MNKRIKKKRKLETAVVMLVAENAMQAEALRNQNKQITELRAIVQ
                     QNAQATNEEFASVKAATLDNQSAIADIAIDVNYIKKNYKRKWGK"
     gene            19990..20565
                     /locus_tag="VBC28_000547"
     CDS             19990..20565
                     /locus_tag="VBC28_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HEO6814549.1"
                     /translation="MAEENVTTETTEQVDTQQETVEHPKQEHERTFTRADISKMMTAE
                     RAKWEAEQSEAIEKARTEGERLAKLSKDERAKEEEQKRLSAIAEREKAVAEREMRIET
                     QSLLAEKGLPLDFIDVVLATTADEVKTNIDNLQSIFDQAVENRVNERLTQKSPRTGNG
                     SVGMTKAEIMAIENDDERLKAIAANRNLFTR"
     gene            20584..21468
                     /locus_tag="VBC28_000548"
     CDS             20584..21468
                     /locus_tag="VBC28_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage capsid protein"
                     /protein_id="HEO6814550.1"
                     /translation="MAENNLNVMSDLGEIKSIDFVNKFSTNINELLKLLGVTRKQELT
                     NDLKIQTYKWSTTLDKTNPAEGETIPLSKVTRAKAKEYTVEWFKKRRATTAEAIARHG
                     VARAINEADTRIMREIQNGFKTNFFEFLKKSTTKVTGTTLQSALSQSWAKIATFNEFD
                     GSPIISFVSPLDVAKYLGDTNVGADASNVFGMTLLKNFLGMQNVIVLPSVPEGKVYTT
                     AVENLVFAYLNVNSGDLGGMFADFTDETGLIGAARTRQLSNLTYESVFFGANVLFAEI
                     PEGVVEATIEAPKVVPGG"
     gene            21472..21828
                     /locus_tag="VBC28_000549"
     CDS             21472..21828
                     /locus_tag="VBC28_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HEO6814551.1"
                     /translation="MDKELLLTEVKLFKGISAKDTSQDDLIKLSINESIDRILAKLND
                     YSDTEMTEIPKRLTFIVRDVAIKRYNRLNSEGTVEDSEEGKTFKWDSYLKEYESTLRS
                     AAIGKVYSGKGVARFI"
     gene            21839..22117
                     /locus_tag="VBC28_000550"
     CDS             21839..22117
                     /locus_tag="VBC28_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6814552.1"
                     /translation="MIYKDRVILVYVDEQDDFLDKRTVEKPSGKIPCMENTFTKSEQM
                     GLFGKYDLNAFKLHLQGRYDGFSKIIYKGKPRLIKGLAHHKNSTVIYV"
     gene            22114..22458
                     /locus_tag="VBC28_000551"
     CDS             22114..22458
                     /locus_tag="VBC28_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HEO6814553.1"
                     /translation="MSLIYRMRGLDRFLRSVERKQKSVRIAVDKELSKSAARIERQAK
                     ILAPVDTGWLRAQIYSEQQRLLHYRVVSPALYSIYLELGTRKMEAQPFLDPALRKEWP
                     VLMANLKKMFKR"
     gene            22462..22821
                     /locus_tag="VBC28_000552"
     CDS             22462..22821
                     /locus_tag="VBC28_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000640604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6814554.1"
                     /translation="MDYSPETLYLKKVKNRLGVLDIPIYFKLPKSDVLEPFIVVGTNI
                     SDLSKTAQTGAVIDDFSLNIDAFLPGDSRLDAEEIKSRMLRLLGRNNQIKAQILVDNS
                     IGREVYRVAINITEILI"
     gene            22833..23465
                     /locus_tag="VBC28_000553"
     CDS             22833..23465
                     /locus_tag="VBC28_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail protein, TP901-1 family"
                     /protein_id="HEO6814555.1"
                     /translation="METTVEITTAKPIKGKNIFYFIQSVDAELGSKAILPAYRTDGTT
                     KLGGDFIDEQTQQGRVLEKATDEHSIDLTSYYTPKDKAIEIVEIAKTTGKSVKVWRVI
                     ADESLKEKGSGTLEGKEVYPAKFGYGKIDEIEYSEGVEKLVEASYTLNIIDSLKDGKF
                     PLTAEDIALLTSVYAYQNPGETTGDYDNIQKKEVSGSLPGAVPGISSERH"
     gene            23516..23971
                     /locus_tag="VBC28_000554"
     CDS             23516..23971
                     /locus_tag="VBC28_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6814556.1"
                     /translation="MELTVAKRSIEIKFDFKTMFKINNRLGTVNKETGERNADGVGTL
                     FYKILERDDTAIVDLVKLSVGSGKKALSEDEVLDSIAELVEEEGSTEALFKEVENDMV
                     ESGFFKEKISKYIESMEKSVKYLEAREDADEAQIQIVKDMIGKMKDAIS"
     gene            24046..24276
                     /locus_tag="VBC28_000555"
     CDS             24046..24276
                     /locus_tag="VBC28_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6814557.1"
                     /translation="MEGLQYKQIAERENLSELALNLRYTLNAKNVNANKLKLDKQRNK
                     IKRTYQNKQDLTNISNDFAERVAKLNLHFQNL"
     gene            24305..28531
                     /locus_tag="VBC28_000556"
     CDS             24305..28531
                     /locus_tag="VBC28_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HEO6814558.1"
                     /translation="MAGTFDGAIFADIGANTKDYEAAMARIVSTTQNAFRKAQDAAVN
                     NSNKMIQMIGQIMAQLANNGQSLGQRLGNAYSTGLKLSLGQIQRIAASIGEKIPEPIK
                     NGFNKAFSIIQNSVQKLSSAIPQPIKSAFTSATSAVVSFSSKITSAVSAAFNSVSSKA
                     STVADKISSSFGSRITSAVTNLATKLSTGLSNGFSRMSSSATSALNGISQRFASASSA
                     GEKLRSTVGSIISAFSLMAIARKGIQAITGAMDGAISRVDTMNRFPKTMALFGYSAQQ
                     SKASIDKLSKGIEGLPTPLDSAVKSAQQLAITTGSLDKGTELALAFNNAMIGYGATTE
                     GAEQALRQFNQSLGSGKIYAEEFNSVSEAAPGLMSKMAESFGFGKNGVQDLKAALSDG
                     SITAQEFADKMIELNKAQGGFAEMAQSSAGGIRTAWKNVHTAVVKGVAGMISAFDEAA
                     KANGMKTIAESINSIKPAINSAFATVNSIMPNLVAAFARISKMVNIDFSPLTSGIKSA
                     FGLLNTALGEFAKTGEVSSATIDRIKSKIASIGPTAIAAWALLNPASAIATIMPLISI
                     IGKIGVALGTVGSALGSFGEMVASGLSTASGLVGAFGRAMSGLPGIFGVAASRGLAVL
                     DGMTTAISSAAILAMSAIGPAAILGLVVVGLGLVNNQFGTQIDKLINTAITKGPGIIQ
                     GLVKGITSKIPALIASGTQLIAKFANALTVLLPVIIQAGVQLITSLVQGIGQNAGSLI
                     SSALKVIGTFVSSIASALPQILAVGVQLILNLARGIVQNIPLIIQYAQQIISNFGSSL
                     QANMPSIISNGIAISTNLNAGIIQMLPAVIQIATQVITGFISGIAQYLPQVIQGGIQI
                     IIMLVQGIIQNLPQIIQAAMQIIQSLISGLSQALPQIISAGIQLVVQLAVAIIKGLPQ
                     IISAGIQLIAGLGKAMLEAIPNALSGVWEGIKGGFSSMWDQITGKSSTSAAKVTSDMS
                     TMTMNVGMQTTTMANQTNIDTTTMLNSISQNTGLANLNATTNATQMATNVNAQTASMA
                     NQGLLNAAGFNEALSSNMTLANTNATNQANQMTANVNNATSQMSLGSLPNFLELSQQA
                     TSNMQTAQANATTSANGMSSGVTGATAGMASGVANNMSGITSTVSTGMNQAANAAKSG
                     GANISNSVNSSFTQAKSTVSSSMSGMNSTVTSGMNSINNTVKSGGNNIKSTFTSSFNS
                     AKSTVSNAMSSIRSSISSGMNSAVSVTRSAMNSVVSAMNAAASGARSAGYNTGIGFAN
                     GLASTAGTIMAIANSIAANVAATIRSAMDIHSPSRVTRELGAYTGEGFALGMQDWMDD
                     INAIGKEYANAVTDQRWETDSQLATSANISSAGVSSSLDNLSDEVRNQQLTSPTFEVH
                     NELVGDEIYTTIKSKEARESNKDNYFNFAT"
     gene            28544..29386
                     /locus_tag="VBC28_000557"
     CDS             28544..29386
                     /locus_tag="VBC28_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000365120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail family protein"
                     /protein_id="HEO6814559.1"
                     /translation="MDLLVQKSDQSFRFSEIGLRILDVDDRSSSLEVDSRTVKGRSGK
                     IFASAKYGTKKVKVNGRLVVASIKDFMTKKDDVNGLLVDSEPFYITKMYPQKENLYDF
                     ELPGMKAGDLDLLNQSHTAWHYRWKVYVSSEPEYTFVGKSSEGLKYNFTIAFETAELP
                     FGETIAQNIVITNGTFNYSGTAPFSQLEYPWSVELTSTGGQSSFYLEIDGRRWAYQYS
                     APLNQGDVIVITGISTTLNKINVTARTNHEYFVLKQKVNKTILYSTNFQGTIKFLNFK
                     ELYK"
     gene            29399..33226
                     /locus_tag="VBC28_000558"
     CDS             29399..33226
                     /locus_tag="VBC28_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEO6814560.1"
                     /translation="MTTFLDHKDNEFEAQAVVKTTNAVNGERSLSGTIYTNEEVLNKV
                     DKGWKLEFDDEYYKIIYAKPTDTGNKIQVEFDAVHQFFYDFDKSSLHKQLNDGSHTFQ
                     AYLNFIFNGSGYTYSLEVVVKSFEKQSFGYKSRLDLFNDIIKSAGVEFFVRGKVVRIL
                     QKTGTDLSTIVRKNFNMNEIIIEKKINDFITYQKGFGAWTDQEDHSKGRLEVEYESPL
                     AKEYGRIEGEPVVDERYTNADNLKSVLKQNVDSSYNIAVKIDIEDLTKAGYQYTQPIA
                     GDYIMAINETLGFKEKIRIVSFESEYDVTGQLINHKVICNDIGTIKKQTVAVSQLSSK
                     INNNQEILDNAVFKANQALASADGKNTNYYGTEMPVDDPKGTLRKGDLLFLTVGDTTR
                     MYFWNGAEWIINSFSDDIDFVKKEITNKIIEVNAAMKLADEQLTEKYNAIVAKNVSQD
                     ELINQAKNLSDSAKKDATEALTNLISESKKLTDKMSALSKTEVEHYSTTNLQLTQLDG
                     TVKGLQTSYEALLKKDGDITQTLANYKQTIDQNSTSITANKKTVDGTLSSLQTQVTQT
                     ANEITTRLSQTNIEALITSKSAVIADNKVKETADSFSREITRVNNTIDNLKIGTVNLL
                     SGTKNWTKNWFNRNNWQSDDDNLTIGNFSLTVLKRKAMWSGISQLYAVKSGENYVFSA
                     YAKSSMDNDVVNLYLLPNGQSPQAKISISFKSITLNTDWQRIVIKFNVTSDGYILPRF
                     ERFNENGWLYIAGYQLEHGNVASDYSEAPADQEDKMTVEFNKINDTVNSHSQLIGKQN
                     ESLTATIQKVDNIQSTVSNVDGRLSRVTQTADGIVTTVSQLDNNILPGTKNFTGWQIE
                     GAVLEDVTQSSYPFVFKKWISGNKVSPLIEYDVKKNQEYTFTAYIAREQAGNLYFYLY
                     DLWNHHITSATPRETIIRDVNSSVIRFRITFIPNRDGKIRPRFAMLASDQGWFMVGGF
                     MLSKGTADLPWSESKQDIKDNVTAINTIVAQTANSWAIKNLTSNGTVLSQLNLTDGNV
                     KLEGKLIHLSGQSIIDNGIITNAMIKDLTADKITAGTIDANRINVINLDASNITSGRM
                     SADLIRSGVLISQNGAMLTDLNTGQIEFYTDNPAIKRITAGYPNQFVKFATGNVEGKG
                     AAGVTVIGSNRWGSESSNDGGFVGIRAWNGKDIDTLDLVGDSIHLASSAYTNADGWEI
                     ITLPNQLSIDARNINHRVTSKVKVGDIWLWKNTSTYVSMKDTINMIIDNLQLLHNNKT
                     TEKGYSYTIPGKI"
     gene            33235..33387
                     /locus_tag="VBC28_000559"
     CDS             33235..33387
                     /locus_tag="VBC28_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6814561.1"
                     /translation="MEEKIKSNLALQIAQLSLDKATLQAQNEQLQLQIAELNQQLDEI
                     TKPGAK"
     gene            33398..33814
                     /locus_tag="VBC28_000560"
     CDS             33398..33814
                     /locus_tag="VBC28_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001873936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1366 domain-containing protein"
                     /protein_id="HEO6814562.1"
                     /translation="MKQWNIVGKPFPIYQDGVVLKTDVRLQGDDGTYLPQELIGNHVD
                     KDNQSLIKLVLETFAKENVVDFAMLESVKDIEQLKADKVTLIKKIAEADEAISKMSQA
                     TKDMQLKVDKAVVELTDLVTSSVSSLSVPSTSMESS"
     gene            33877..34038
                     /locus_tag="VBC28_000561"
     CDS             33877..34038
                     /locus_tag="VBC28_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6814563.1"
                     /translation="MTYTFKKDHLIVDVWYRRVANGICDFEAVPKLFNLRDIVLELLS
                     QKVTDKESE"
     gene            34048..34338
                     /locus_tag="VBC28_000562"
     CDS             34048..34338
                     /locus_tag="VBC28_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6814564.1"
                     /translation="MIDFVQVGAFAGAALSILGVWGFIVNPFKKAMEANEIAMAQLKD
                     SLKELAYELKNIDRDREITKAVIDRHEERLGRVEDDIIINKEQIKTLFNKEK"
     gene            34340..34591
                     /locus_tag="VBC28_000563"
     CDS             34340..34591
                     /locus_tag="VBC28_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000611525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HEO6814565.1"
                     /translation="MINWKVRIKNKAFWSAIIPAIFLVVQAVANVFGYTLELSDLGNK
                     LLVVVNSVFSVLVIAGIVTDPTTQGVSDSSRALSYTEPK"
     gene            34711..35904
                     /locus_tag="VBC28_000564"
     CDS             34711..35904
                     /locus_tag="VBC28_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HEO6814566.1"
                     /translation="MTFLDNIKQGCLDGWTKYKILPSLTAAQAILESGWGKYAPHNAL
                     FGIKADSSWTGKSFNTKTQEEYQAGVMTDIVDRFRAYDSWTDSIVDHGKFLNDNPRYK
                     AVIGETDYKKACYAIKDAGYATASGYAELLIQLIEENNLQSWDAETIKNKEETMTTAN
                     EIVQYCVNLANSGMGVDKDGAYGTQCADLPCFIVKNWFGIDLWGNAIDLLNSAAAQGL
                     EVIYDAPGVNPKASDLFVMEVAGSPYGHTGAVIEDSDGYTIKTVEQNIDGNWDSLQVG
                     GPARFNTRDFTGVVGWIRLPVDHTNQTVDTSPQTSDTLVETPKSGTFMLDVAEINIRR
                     WPSLASEVVGSYKQGDNVTFDSEGYANGYYWISYVGSSGKRNYMAIGQTDKDGKRISL
                     WGKIK"
     gene            36270..36785
                     /locus_tag="VBC28_000565"
     CDS             36270..36785
                     /locus_tag="VBC28_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6814567.1"
                     /translation="MKSKQLVLGQIDLNMCRDFNLAQAMDYNSKTKRLYNKGRGFAIV
                     LVTIQNLTFAIPLRSNIPKKYQLKYKLRDSKKYGCVEGLDIGKALVVEDPKYILNRTF
                     KLREQVDYFKIVDNDILIVNKLIKAIIDYNQAVANSDQNKLTDPKRFKFSTFPNYTDR
                     LKSITAADYLY"
     gene            36913..37113
                     /locus_tag="VBC28_000566"
     CDS             36913..37113
                     /locus_tag="VBC28_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEO6814568.1"
                     /translation="MSQEKLKSKLDQAKGGAKEGFGKITGDKELEAKGFVEKTIAKGK
                     ELADDAKDAVEEAVDAVKEKLK"
BASE COUNT        13125 a         6069 c         7746 g        10345 t
ORIGIN      
        1 aatactttta ctcaaaaaat tcgcaaaaaa gttcgctttg caattttaat aaggagggta
       61 aatgaaaaaa cgtaaaagag atatttctag tcttaaggaa cgaattaatt actttcaaga
      121 tgtctatgac tatatgctgg tcaattctga aacttcattt attgaaaaag attggtctga
      181 taatgtccga atcatgagta tggaagatat caaagaagaa attcttgtgt cacaaaatcg
      241 tcttgaagaa cttcaaaatc aaacttttaa gaaaaatgtt ttgaaattag tgcaccgatt
      301 attccgccga tgagcagatt gataatccaa attatagttt tgccccgcaa agtagattcg
      361 aatgtagacc atagtccaac gatatttgaa cctcttattt tccatttcgg tacatctgtt
      421 ttcaatgttc cgtttaatgc ttgggtcaaa aaattgaaaa aaaggtttgc ttttacggac
      481 gtggacatac ttaatttgac atattcgtta tcatcaaacg ctttcgcttc atcttttccg
      541 tttaagaaat attttggtgt taaattattt cgttccatca gttttacaat atgagggttg
      601 ccaatatagt cacctccata aagtaatttg aataagtgtc cgaggtcgat tttcttatca
      661 ttatcaatag cgataggatg accgcctaaa actttaaaac gttgctcaag agcattaaaa
      721 ccaaatacgc tatctctcat gttccaatga atccagattt tatctaagtt tttatcaatg
      781 aatgcgaaaa aatctgttaa taattctttt tcaatatctt tataagtatt gtcagtgata
      841 ggaatgttta acatttcaga atattggtga atagagaaat tattggtttg agcgttatta
      901 aattgtcgga tggaaataga tgcaatacga ggactttttc cttttaaggc attaaagtcc
      961 tcgcaggaat aatgaataat caaatatttt tctggtctat cttcaatgtt ttttagtata
     1021 gaactagcat gtttgttatc ggtatatcgt gacatatcaa cctcttattt attattttat
     1081 ttcattatat cagaaaggag aatacatgag ccaacaacat cgaaaatggg ctgagctggt
     1141 agaggctcgg ttgaaagaaa aaggctggaa caaagctgac ttgacacagg cgattggctt
     1201 gcgaagccaa gggactatca cggacttact aaaattaggt aaaggaagtg cggatcttaa
     1261 aattcgcgtt tcaaaaatcc tcggcatccg tgagccatgg gaaaaatttg aggagggata
     1321 gagtggaaat atcagtagaa actccgttga gaacgcaaaa aaagtccgat tgcagtcgga
     1381 ctcaaaacaa aaaatattta ctttatttta acacagaagg gaatgaaaag ctatgccaaa
     1441 agcagaatta gtctacagac cagctaagca gtcggaaaaa gccgactatg gcgattatgt
     1501 gcatctttgc caaatctggg agggattaac catcggaacg gcaaaggttt gggcagcaga
     1561 aatgagaaat catccagatt ttaaacaatt tatcaataat ccaacgcata gaattgtatt
     1621 catcaattac gagggattta agttgtttgt tacttggaag tctagaaata gatatagacc
     1681 taaaaaggaa acattagccg aaatgataga aaatatcaaa cgtgaaaaac agttaggagt
     1741 ataacataat gaagaaatta tttaaaaaat tatttgtaaa gaaaaatcag gttatagaaa
     1801 cacgtcaaaa atggacaatc gaaacacatg gctgggaagc taatgcacga cgttacgaca
     1861 atatgattga acgtaataat agagggcgta catgttaaaa gaagtattac tagaaaatga
     1921 actgttgcgt gatgaaaata gacgactaac taatgaaatg gcagattatt attttacgaa
     1981 cgtagcaaaa gcaaatttat tggatattat cgtcaatgaa ggctacatct tagatagcac
     2041 actggaaaaa tgtattgcta agctagataa cgttgataga caaacactag aaaaaaattg
     2101 gagtgatgat aatgattgaa gttgaaagac aaccaaaaaa tacgtactca cgaattagat
     2161 gttcaaataa tatttatgat gaaatcgcca atattgcaaa tgaatgtgat ttaacactaa
     2221 aagaagtaac tgatgcacta ctaaactatg gtcttgctaa cgctcaaatt attagttctg
     2281 aaaaagtagt taccgaaagt aaattagtaa taggagattt tgacaatgac aataacaatt
     2341 aataagttag aaatcgaaaa cgtaaagcgc attaaagctg taaaagttga accatcatcc
     2401 aaaggattaa ccatcatcgg tggcaataac aatcagggta aaactagcgt gctagacagc
     2461 attgcttggg cgttaggtgg taataaatat aagcctagcc aagccgtgcg tgatggctcc
     2521 caagtaccac caacgcttaa aattaccatg tcaaatggtt tgattgttga gcgtaagggt
     2581 aagaattcta gtttgaaagt tattgatcct aacggtcaaa aaggtggtca acaattgctt
     2641 gatagctttg tagaagagtt agctattaac ttaccaaaat ttatggacag tacgccgaaa
     2701 gaaaaggcga ctattctcct acagatcatc ggtgttggcg aacaactcta ccaactagaa
     2761 caacaagaga aaaccaagta cgacgaacga actgcgattg gtcgaattgc tgaccagaag
     2821 aagaagtttg ctgacgaatt accaagctat ccagatgcac caaaagattt ggtctctatc
     2881 acagatctta ttcaagaaca acaagcggtc cttgccaaga atggagagaa cgctcgcaag
     2941 cgtcaaaatc ttgaacaaat cacgacgaac tttaacaatg ctgaattgaa agtggcacag
     3001 ttacaggaag cgctagctgc tgcaattgat gagcgcgata tagcaaaaca ggacttagct
     3061 attgcccaaa aggacacgat ggacctagta gatgaatcaa ctgctgaaat tgaagggaac
     3121 attgctcgta tcgatgaaat caatcgcaag gtacgtgtga atcttgataa ggaaaaagcg
     3181 gagatggatg ctaaagctca caaagaacag tatgacttgc tgacaagtga gattgaagct
     3241 atccgcaagc aaatcacaga cttgttagat aatgcagact taccactacc aggtttaacc
     3301 gtggcagatg ggaaattact ctatcaaggc caagaatggg ataacatgtc tggcagtcaa
     3361 caactgattg tatcgactgc gattgtccgt aagctcaaac cagactgtgg atttgtcctc
     3421 gtggataaac tggagcaact ggaccaaccg acgttggaat tctatggtca gtggttagag
     3481 caggaaggac tacaagccat tgccacacga gtttctactg gcgaagagtg tgccatcatt
     3541 atagaagatg gatactcagt ggaaaataaa gccaatagct ttaaaacagc agaaggtggt
     3601 agttttgctg aaacagtagc accgacttgg caaggtggat tttagaaaga gaggtaaata
     3661 tgaaaattac aagaggtaaa cgggcaaggg cacaacgtgt catcatttat ggaccagaag
     3721 gaatcggtaa atctagtttt gctgctaatt ttcctgaacc attgttcatt gatacggagg
     3781 gatcaacaga taatatggat gttgctcgca tggacaaacc gacaagctat actatgctaa
     3841 aaaatcaaat tgcatggatt aaagcgaatc caacatgttg caagacactc gttattgaca
     3901 ctattgactg ggcagaaagc ttaataatag atgatgtctg tgctcaacat ggaaaaaaag
     3961 gaattgaaga ttttggttgg ggcaatggtt acacttacac aaaagaagaa gttggacgtt
     4021 tcctaaatat gttgcaggaa ttgatagagt taggtatcaa tgtcgtcctt actgctcatg
     4081 cccaaatgcg taaatttgag caacctgatg aaatgggagc ttatgatcgc tgggaactca
     4141 agctaggtaa aaagacaagc tcacagactg caccattagt taaagaatgg gctgacatgg
     4201 ttctgttcgc taattataaa actgtagtga tgacagctga taacaacaag aagaaagcaa
     4261 ctggcggtgc ccgtgtctta tatacacaac atcatccagc atgggatgca aaaaaccgtc
     4321 atggattaca agaagaaatg ccacttgatt acgctggtat cgcacatata ttcgcacaag
     4381 caccaagtca accagtaaca gaaacaccac cagtacagca aactacgcct gctcaatccg
     4441 tacacgaccc cgcacatgag catcgaacaa cgtctcaacc gcaacaagtt ccagagaatg
     4501 aaacaggtca acaagttgtg tatccatcat cattgccaca ggcattaaca gacctgatga
     4561 tggccgaaca agttacacca gatgagttag tagcagtagc aaatattaga ggacacttcc
     4621 ctccattgac tcctattgaa aactttccac cagattactg gaacatgatt gtagcaaatt
     4681 ggggagccac attggaagtt attaaaacac aagtccgagc aacagaacag ccctttacag
     4741 tggaaggctc atagattttg ggaattagaa ataatatcga ggtataacaa aacatggata
     4801 aaacaatcaa attagattta tcagctattg gagaaggagg tttacaagaa aaagtggata
     4861 aggaacttga aaaagttttt gacaatatcc tagacccaaa tactgatatt aaaacaaaac
     4921 gaaaacttac catcacacta acaatgactc ctgatgaaac acgagaggtt gttagcacat
     4981 caatggaagt taagtcaacc ttggcgccac aaacaggtgt agcaaccact gtgcttgtcg
     5041 gtcaaaaaga tggcaaagtg tatgctaacg aacttaaaag taaaatccct ggtcaaacat
     5101 attttgacga agaagcaaca ttacgaacag atatcgggca gccgattgat gaccttgaaa
     5161 gaggcattaa tgaagatatt attgatttta acaaacaaaa gaaagtaggt aactaatatg
     5221 tcagaaaaca ttaaagaagc tttggaatat gcagtaaata tagctaacca aatgcctgag
     5281 actatattgg gtaatgatgg caaagagtat tatgatcgca acaaatattc tttggtcgaa
     5341 ctcaaaacta agtattatcc aaaaacctta aatttaaaca cgcttgacag tttaattgat
     5401 tatcttaaat cagatatgaa caatattaat tccaagcgtc ttatggtcat tgtagaaggc
     5461 cctcgtgaag ttactgtttg cgaagaagat gacgatgaac ttaatcgaaa tgtgttagta
     5521 actgtggaag caattattcc aaatgttcaa tttggccgct atgattcacc ttctgatttt
     5581 aatattagcc tgcaatcatt attcgttaat gctgacgatc gtaacacagt tattgaattt
     5641 gctagtgcat taaaaatcga aaacggctca gaaattgttg atgatggcat tggtcaaact
     5701 gcaacaatta aacaaggtgt ggcaagcctt gcaaaagcta aagcgccaaa cccagttaca
     5761 ttacgcccat accgcacatt ttctgaagta gaacagcctt caagccaatt tatttttcgc
     5821 attaatcaat ttgctaatat ggctttgttt gaagcagatg gtggaaagtg gcgcttagat
     5881 gctatcaata atattgctga ttatttaaaa acagcgctta ttgatcaaac taacattaca
     5941 attttagctt aataaaaaat aggagaaata aaatgactga atacaataac aactttgaac
     6001 gtgaatttgg atgggatgac acaatccaag aagatgcaaa agaatttatt acattacaac
     6061 ctggtgacta tgtcttcaca gtaacagact ttgaacgtgc acgccacaca ccaaatccac
     6121 aaaaaacagg gaaattgcca gcatgtaata aagcagtaat ttctcttgaa attgaaactg
     6181 atgaaggtgt tgctagtttg aagcataatc tatttttaca tagctctacc gaaggaatgt
     6241 tatcagcatt ctttggggca attggtcaga aaaaacacgg tgagccatta aaaatgaatt
     6301 ggaacacagt agttggtgcg acgggtgtat gtagtgttaa gaatagaaca tataacgaca
     6361 atttttataa tgaagtgaaa tcgatgattt atgctgatag cgttgactgg actaaagtat
     6421 tgaatgcaaa tactcaacca caagcgccac aaccaacata tcaacaacca gctcagcaaa
     6481 caactaattt tgcacctcaa caacaaccag cacctcagtc atatcaacaa ggtcaaatgc
     6541 aagcacctca acaacaaggt ggatggggag gattctaatg cagcttagac cttaccaaga
     6601 agaggcaagg tcagcagtgc aacaagagtg ggaacaaggc aagaagaaaa cccttcttgt
     6661 ccttccaact ggttgcggta aaactattgt cttttcgaaa attatcgaag accgtgtgaa
     6721 gctcggagag agagttttgg ttctggctca taggtctgaa cttttagaac aagcaagtga
     6781 taagctaatg acagctactg gattagggac agcattagaa aaagcagaga atacttcttt
     6841 agggtcatgg ttcagggttg tagttggatc agttcaaacg atgcagagag agaagcgttt
     6901 gagtaaattt ccaacaggtt acttcgacac aattgttgtt gatgaagcgc atcatgctat
     6961 ttcagacggt tatcaacgtg tgctgaatca ctttgaagaa agtaacgttt tgggtgtgac
     7021 agctacacca gatcgtggtg acaaacaaaa tctaggtaaa tatttcgaca gtttagctta
     7081 tgagtattct ttagtagatg ctataaagtc tggttattta tcaaaaatta cagccgtaac
     7141 aattcctttg accttggatt tatcagctgt cagtcaacaa gctggtgatt tcaaagcaag
     7201 cgaaatcgga acggcactag acccgtatct ggaacaaatt gcggatgaga tggtcaagca
     7261 gtgcgcgaat cgcaaaacag ttgtattttt accacttgtt aaaacttccc aaaaattccg
     7321 tgatattcta aaccaaaaag gattcaaagc agcagaggtc aatggagaat cgaaagaccg
     7381 tgctgaagtc ctagaagact ttgataatga taaatataat gtactatgca attcaatgct
     7441 tttaactgaa ggatgggatt gcccgacagt cgattgcgta gttgtactga gacccacaaa
     7501 agtaagagct ctttatagtc aaatggtcgg tcgtggcaca cgattagcgc cggggaaaga
     7561 aaatctactg ttattagatt ttttatggca taccgaacgc catgagcttt gtcgtcctgc
     7621 tcatctaatt gcaaaaacac cagaagttgc acaaaaaatg gtcgagaaca tgaccgatga
     7681 aactgaagta gcttttgaat tgttagaagc tgaagaagta gccagcaaag atgttgttgc
     7741 agagcgagaa gaagcactcg ctaagcagtt agcagaaatg cgtaaacgta aacgtgcatt
     7801 agttgatccg ttgcaatttg aaatgtctat caacgctgaa gacttagtgg actatgtacc
     7861 ggaatttggt tgggagatgg cgccagcttc tgaaaaacaa cttaaaacac ttgagaaatt
     7921 tggcatattt actgacgaaa ttgggaatgc tggtaaggct gccaaattat tagatagact
     7981 ggataaacgt cgtcaatcag gtctaacaac tccaaaacaa atacgaatgc ttgagcgata
     8041 cggtttcaaa aatgtaggta tgtggaaatt tgaagtagca tcaaacatga ttaatcgtat
     8101 tgctgctagc ggatggcgcc taccaaaagg catcaatgct cgagaatatc aaccagaata
     8161 ggagaaaaaa tggaaaatat taatgaacag attgaaaagt ttatttcaaa ttttgctgat
     8221 gaagcaatcg aaaaatccga aacatattcg gaggcaattt tatatgtcga taaacactca
     8281 agtctcaccg agtttgggca agtagtaaaa aaagctattc aagaaaaaat cagagatatt
     8341 gccttgaata gcagaattat taaatagctt caatttttat aacagcattt gaagcgtaat
     8401 aaatttgtgg gttttctaag tcaaagaaaa attgcgagtt agcaattaat tctaagaagc
     8461 taggaattaa tccatcatga acatggttcc ataatgaata tatttttgat aaggacatag
     8521 catcatttga ttttactaca ctttgaaaac tctgttcttc gtgaaatata acaatagaac
     8581 catctgaaaa agtaattttt atttcaggca tatagttgac ctcctttctt taggttaatt
     8641 atatcaaatg cagaaaggat aacatgtgca gaaaggataa catggcagag aatgatttta
     8701 atttgctacc gttgctggat tatatcaatc ctgccacggt agactatcaa acatgggttc
     8761 aagtaggaat ggccctaaag catgaaggtt atacagcaat ggattgggat gtttggtcac
     8821 aatctgatag tagatataaa aaaggtgagt gttttgcaaa atgggatagt ttccaaggca
     8881 atggctttgg gactatcaca ggggcaacaa tcacacagct agctaaagat aatggatgga
     8941 catcatcaga gtatcgtaac agcgatgatg ctcatgaact cagctgggac gatacaatcg
     9001 atcgtgatta taagattgtc gataaaaatt ggattgaatc gaaagagatt caagagccaa
     9061 gaaactggaa tccagttcaa gatttaatta cttacataga taccttattt gaatcaactg
     9121 acaaagttgg ttatgtaaca gagacctatc caattaccct tgatacggga gagattgttt
     9181 ataaaccaac aaaaggagcg tatgacagga ctgctggtca actgattgaa tcactccaaa
     9241 aaaatcctac tgacttagga gcagtatttg gagatttcaa agaagaggct ggtgcatgga
     9301 ttcgttttaa tccactagat ggaaatggtg tcaaaaacga caatgtaaca gactttagat
     9361 atgccttggt cgaatcagac acaatggaat taggtaaaca atatgctttg tttaaagagt
     9421 tagagttacc aatagcaacc ttggtccata gcggtaaaaa atccttacac gctattgtca
     9481 aagtagatgc tagagattat caggagtacc gcaaacgggt tgattacatc tatcaaatct
     9541 gtaagaaaaa cggtcttgat attgacacac aaaatcgtaa tccaagtcgt ctatcacgaa
     9601 tgccaggtat tatgcgaaac ggtcacaagc aatttttgat tgatacaaat attggtaaaa
     9661 ccaattatga agaatggtat caatggattg aagatttaaa tgatgattta ccagagcctg
     9721 aaacgctagc tgatgaatgg gataatttgc cagagctggc tcctgaatta attaaaggag
     9781 tgttacgcca aggacataaa atgctaattg caggaccatc aaaagccggt aaatcatttg
     9841 cattgattga gttatctatt gctttggctg aggggcagaa atggttaggt tggcaatgtg
     9901 aacaagggaa agtcctctat gtcaatttag aattagacag accatcagct ttgcaccgct
     9961 ttaaagatgt ttatgatgcg atgggactac ctcctactaa tgtggcaaac attgacattt
    10021 ggaacctccg tggaaaaaca gtaccaatgg ataaattagc ccctaaactc atcagacgct
    10081 cactgaagaa aaattaccaa gcggtcatca tagacccgat ttacaaggtg ctaactggag
    10141 atgaaaacag tgctgatcag atggcccatt ttacaaatca atttgataag gtggccactg
    10201 aactagggtg tagcgtgatt tactgccacc accattcaaa ggggagtcaa ggtggtaaaa
    10261 agtccatgga cagggcaagt ggttctggag tatttgctcg tgatcctgat gcattgattg
    10321 acttagtaga actggaactg actgatgagt taatcaagca acgtgttgaa aaggcatctg
    10381 ctaaggttta tcagaaagct ttgcaggaac agaacctttc ttactatcag catgaagtga
    10441 cattagacga tttagaaagt cgttatcaga tgcaacaaca ttttgacaaa gctatcaagg
    10501 atatcatggt taaacagcca tatctgaacg aaatcaagag aattactcat gctattgaaa
    10561 tcagtacagg ctggcgagtt gaagggactt tacgtgagtt tgctaaattc caaccagtta
    10621 acatgtggtt tagctatcca gttcatgaag tcgatacaac gggtgtcttg gctgatatat
    10681 cactagaaga taatgtgccg acttggaaga agaattttga gaagaagagt ccaagagagt
    10741 ccagggaaaa gaaatctcaa aaagtagaga ctgcaattaa ttcattgaat gatggaatag
    10801 agccagtcac aatcgataac ttagtcgaat atttttctac tgaagataag ccagtttctg
    10861 aaaaaactat tcgtagatgg ataaaagaaa acggtaaatt tgaagttaaa aataaacaaa
    10921 ttttaccaat agaagaacct aaaaccaata aaagtttgtg aatatttcac agagggacag
    10981 ggacagggac agacaaaatc gagggtcaaa ctcgaaagtg tccctcgaaa atgtccctga
    11041 aaaataggga caaactcgaa aatgtccgtg tccctattac atgattttca aggacaaaaa
    11101 tagggacaaa ttcgaaaatt tctcgaaaat gtccctgact tttgagggac aaaattgagg
    11161 gacagaatat tctctttctc cgaaagaaga atatttaggg aaatgtccca attggtcaat
    11221 aggtacatga acaggaacaa gggggctatg catccgcccc ttgtaaccct gtaaccatgt
    11281 cccttgacat tgactatgcg cgaaaataaa aaagatggga agtgacaaaa attaaaattg
    11341 aattttttat cccgatgaag caaattccaa caaccaccca ccagcaaaaa aaggtgagag
    11401 ttgttaatgg taaaccacag ttctatgaac cggattcgct gaaggaagtt agggccatgt
    11461 ttatgagtaa atttgcaccc catgctcctg agaaaaaaat gaccggtcca ataagactta
    11521 ctgtcaaatg gttattccca cgtattaaaa aatcaataga tggtcagtac aaagatacta
    11581 aacccgacac agataatttg aacaagctac ctaaagattg catgaccgaa ttgggatttt
    11641 ggaatgatga tgcgcaggtt gcttcggaaa ttattgagaa gttctgggct aatacagtag
    11701 gaatatacgt aaaggtggaa gagttatgaa aattgattat attgattttt ttgaacgagt
    11761 ggtccctggc tggatgagag aaagtaatgt caaaatacag gaagttggat ttaatactga
    11821 agcttattgg gtatgggcaa atcaatctat tgttgcaatc tgtgaaaagt acggaaatga
    11881 cagtctgatc aatgggcagt ttcatctgat ttgggaatgg ttggaggata aagcaaacgg
    11941 aggttaacga tgataccaaa atttagagca tttaataaaa agaccaaaaa gatgtatagc
    12001 gtcgatggct ttaaatcaag tgaacgcaaa atatacagat gcagcttagc agatgatgag
    12061 tttcgccccg gacatatgga gacattccat tttgtagagg ataatcttga cgattatgtc
    12121 cttatgcaat caacaggaga gtttgacgaa aatggcgttg agatttggga aggggatata
    12181 gttttaacaa cacgtctcat agattacaca tataaaaatt ttaaaggtgt agtaaaaatg
    12241 ttagaaggtt gctggttaat tgacacaggg actgatgctg tctatttatg gtcagaaatc
    12301 gaagaaaacg aagtcatcgg aaacatatac gaaaatccag agttactaga aagcgtggaa
    12361 gaatgaaact atcaacattt attgaattat tgcaagaaga cgatatgaaa cctgattggg
    12421 aagttacttg cggaattgcg attgatcccc aaaaaagacg aatttggttt cctgaacctc
    12481 acgaaagcgt ggacgaatga ttaaaacaaa aatttttact acttttggca caggcgataa
    12541 ttatacatgc gcagatgatc aatgtaataa atttatagcc aacaacccta aaatcaaacc
    12601 aattgacttt aagtttcaag gaaatgtcag tgggtatgct gatggaggca ttaatggaag
    12661 cgactattat gaagctatcc tcatgattta cgaagaaagc gtggacaaat gaaaattgat
    12721 tttgaagaca aaataaaagg tgtttatggc tggacaataa aagatggaaa agtaaaccct
    12781 ccaaaacaca atttaccgaa aagtgttaaa gaaagagctg attatttttg ggaaatgaca
    12841 gagcatggtt taacttttct tggcgcattg gactatattt ttatggatga aaagccggaa
    12901 ggttatgata cttttgcaac taaggagtgg ctagaaaaaa ccaaagaatt taacgaatgg
    12961 tttcacaata gccctaacat ggcacaagct gaaattgcaa tttacttgct ttatggtaac
    13021 aacaaatgaa acaagcatat agtttttata cacaggaaat catttgttta gctgtgttcc
    13081 ttctgatatt tggtggttta cttggtggat ttatcataca aaattattgt gatagctact
    13141 atcaaccgca aatcgaagga ctacagagac agttagacaa atcgcacgag cagttagcta
    13201 aaaagactaa gcggattgct gagttgactg gaaatggagg atgatggtgg ttcaaaaatc
    13261 gttattatca tcagacaaag attattggga aacaccgcaa aatttttttg aaaaattaaa
    13321 cgaagaattt aattttaatt tagatgtcgc atcatcagac aacaatgcaa aatgtaaaaa
    13381 tcattttact gttgacgatg acggattatc tcaagattgg acaggaaatg tattttgcaa
    13441 tccaccttac ggcagagaaa tagggaaatg ggttgaaaaa gcttacaaag aatcacttga
    13501 accgtataac aacgtcattg ttttgttaat cccagctagg actgatacaa aatattggca
    13561 tgattacatt tttggcaaag caacagacat tagattttta aaaggtaggc tgaaatttac
    13621 gatcaacgga aaagaaaacc atccagcacc cttcccgagt gcagtaataa tttattaatc
    13681 gaggtaacaa atgaagattg aagaagcgaa agaattagta gataattcaa atttttatgg
    13741 aaagactatc agtgttataa aagccgaggt tcacgccatt atagaccagt taaacccacc
    13801 aaaaccagaa atcccacagt ttgtggctga ttggtatgaa aagcataaag atagcttgga
    13861 atactacatt tgggaataca taaacgaatg ggacaatcaa gaacaagatg attttttcaa
    13921 ttttatgaat tatggcacat ttaaacctat ccagacgtta gtcaacatgc accagtttgg
    13981 gtatgaaatt gagcaaaaga agctttacac tgttgacttg ccaaacggac aacctttagt
    14041 aagcggcaaa aacactttgt attttagtca aaatctagca gccgaaaatg taaaactcac
    14101 cgaatccgaa attcgaaaag actttgattg ggcatggcag tttgctgaag aggtgacgga
    14161 atgagtagat tatccaatgc acagttaaaa gcttttgatg agtggttgtt tgattatcgc
    14221 gatattgagc gtaaaattgc aattagaaaa cttgagctgc agacgcctgt tagtacagat
    14281 atcaatgtag gcgggggcaa gtctaatcta gtagctaaag agacagaaga tattgtcata
    14341 aagtggtcaa aggacggtaa aatcaaaagt tatgaaaact tcagagaaag tgttgatagg
    14401 gttaaagcac tgcttgacga tgaattgaat tatatttttg atctaaggtg gggaactggt
    14461 tctaataata cctgggagga gatagcttat aagattcata cgtctagagc aggaacctac
    14521 cgaaaaaggg atagaatttt gactctattt gcacaacaaa ttggaaaatt gtaagtttga
    14581 gacaaaaaag ggtggttttc tcactttttt gatgataaat tggtagtatg agtttgaagg
    14641 caaggcaaat agtgtcttgt ctttttatta tgtcacgaag gaggtgatgg aaaattgagt
    14701 aaattaacac taaaacaaaa gcgttttgcg gatgagtaca tcatctcagg aaacacagaa
    14761 aagtcagctc ttattgctgg atatagtaaa aattacgcta gagcacaagc acataaattg
    14821 ttggcaaatg ttggcattaa atcctatata gatgaaaggc ttgaaaagct tgaatctgca
    14881 aagattgcca cgcaagaaga agttttacaa taccttacat caatcatgcg aggcgatcag
    14941 caagaaaaaa cactcatcag catcggggag ttaggacagg aaattgtaga tatcgacgtt
    15001 ggtgcaaaag atagaatcaa ggcagccgag ctactaggca aacggtacag gttatttact
    15061 gacaaggttg aaatggatgt cagctcagat gtaaccatta acgtaggtga gtgggatgac
    15121 gattaaacag cgccctaaaa ttaatatcat aatcaagcat cctagcaaag tttttaacaa
    15181 acatatctac gacaaacttt acaactatag caacttcacg gaagttcatt atggtggggc
    15241 gtctagcgga aagtctcacg gtgtttttca aaagataatt ttaaaagcac tcaatccaaa
    15301 gtttaaacat cccagaaaga tattagttct cagaaaagtc ggtgcaaccg ttagagactc
    15361 tgtatttgct gatatcatgt ctactttgtc gtatttcggc atcttggata aatgtaagat
    15421 aaatatgtcg gcttttagaa taacgctccc gaatggctcg gaactcattt ttaagggtat
    15481 ggataaccct gagaagatta agtcaattaa ggggatatct gatgtcgtca tggaagaagc
    15541 tagtgagttt acgcttgatg attatacgca gttgactttg cgtttaaggg ataagaaaca
    15601 tctagagaag caaatatact tgatgtttaa ccctgtgtca aaagtaaact gggtttataa
    15661 agccttcttc gttaagacac ctaagaatac agttgtctat caaacaactt ataaagacaa
    15721 ccgcttctta gatgatgtca cgagagaaaa tattgaggaa ctagccgata ggaatgaagc
    15781 ttattacaaa atatatgctc ttggccagtt tgctacactt gataaactaa tttttcccaa
    15841 gtatgacaag caaatattaa acaaagacaa gttatcacac ttgccttctt tttttggttt
    15901 ggactatggg tttatcaatg acccttcggc atttttgcat gttaaaatcg atgacgcaaa
    15961 caagaagtta tacatcttag aggaatatgt cagaaaaaat ttgaccaatg ataaaatagc
    16021 aaatgctata aaggaccttg gctatgccaa agaagaaatc agaggagatt cggctgaaaa
    16081 gaaatctaac caagagctga ggaatttagg tattcctaga atgattgatg ttaccaaagg
    16141 tcctggaacc gttatgcaag gaattcagta cctgcttcag tatgattgga ttgttgatga
    16201 aaggtgtgtc aagactattg aagaactcga aaattacact tggaagaaag acaagaagac
    16261 taatgagtat atcaatgaac cagttgacag ttacaaccac tgcattgacg ccataagata
    16321 tgccgtacaa gacagaatat atcagtcggc agatagaagt aagcgcatga agaacgcgaa
    16381 gtactatttt taggaatgag gtgagacttt ggaagaaaaa caatttttag taggaactcg
    16441 ttttaatgag aatgctaata agcaatttat tatgctgcaa gaagactttg aagcaattga
    16501 ctatgaatca aaactttgga tagatcaact taagaattac atttcaagat ttaaagcaga
    16561 acaattagag cgtttgaaag agttaaagcg atactatctt ggggataata acatcaagta
    16621 tagacctgct aaaacagata aatacgcagc ggataatcgt attgcaagtg attttgctaa
    16681 gtatattacg gtatttgagc aaggttatat gcttggcgtt cccgttgagt acaaaaatga
    16741 aaataaagac ctccaggcag ctattgacct tatgtcggtc agaaacaatg aggattacca
    16801 caacgtcaaa ataaaaacag atttatcaat ttatggaaga gcctacgagc tgttaactgt
    16861 tgaggaaata gatgacaaaa aaaccgaagt aaaactctac cagctcccag ccgagcaaac
    16921 ttttgttatc tatgatgata cataccagcg aaattcgttg atggccgttc atttttacga
    16981 catagattat ggctcaggta aacgcaaaca gattatcaaa gtatatactt ccgacacaat
    17041 ttatacctac gaagactata atttcgaaac aaaaggtatg cgcttaaaag agcatgaaga
    17101 acattttttt aaaggtgtac cagtcaatga atatgctaat aatgaggagc gtacaggggc
    17161 ttatgagtct gtacttgata atattgatgc ctatgattta tctcagtcag agcttgctaa
    17221 ttttcaacaa gattcagtca atgcgttgct tgtaatagct ggtaatactt acacaggggc
    17281 tgatgagaat gactacttag atgacggacg attaaatcct aatggtcgtt tagcgatctc
    17341 aattgggttt aaaaaagccc aagtgttaat tttagacgat aatcctaatc caaatggcgt
    17401 taaaccacaa gcgtactttc tcaaaaaaga gtatgatacc gccggtagcg aagcctacaa
    17461 aaacagacta gttgcagaca ttttgagatt tacttttacg ccagatacac aagatatgaa
    17521 attttcagga gttcaatctg gtgaatcaat gaagtataag cttatggctt ctgacaacta
    17581 tcgcgagaag caagagaggt tgtttaaaaa aggcttaatg cgacgcttac gcttagcagc
    17641 taatatctgg gccatcaaag ggaatgaagc aactacatat agccttgtaa atgatacgag
    17701 tatagttttc acacctaatc ttcctcaaaa cgataatgaa attgttacag ctgcacaaaa
    17761 tctctatggc attgttagcg atcagactat ctttgaaatc ttaaatactg tcacaggagt
    17821 agacgctgaa gcagagcttg aacgtttgaa agaagaagct gataaaaagc aatctttacc
    17881 ggagcctaga ttggtaggtg atgctagtgg tcaagaagaa ccaacagcag aaaagcctta
    17941 attactggca aaaacgtcaa gaagatattc taagttattt agacagaact gatttagacg
    18001 tcttttcaga actgcaaaag ttatataatg aacaggcttt tgagttacaa aaggaattgt
    18061 ttgattttta tactaagtat tctgaagaga acaaaatgac ttatcaggat gtcgttaaaa
    18121 agctacgtca tgaagattta tcagattacg tagctaatgc taacaagtat cgtaaacaag
    18181 ctgagaaaga ccctgagttg ctaaaacgac ttaatgagca gtatgtatca gcaagagcta
    18241 caaggatgga tgcattaaat cttgaacttg tttatcgcgc agggatacta aaaggtgtac
    18301 ttgattcagt atttgaaaat catttaaaaa aagctgcctc atatgcttat aaaaaagcaa
    18361 tgggtggacg gtcagggaca atcaatggtc cagttttaga agaactggtt agaaccccgt
    18421 ttgatggtta taactactca gaacagttgt ggggcaatac tgacaatctt gtcaagaatc
    18481 tccaaaagaa attaaaacaa ggttttgttc gtggcgagca tcctagagcg atagccagag
    18541 atttagctaa gcaatttaat gttgctaatc acagggctga aacactaatc agaactgatg
    18601 gaactatggt gattaataat gctaccgcta ggcgctactt gaatgcagga cttaagtatt
    18661 atcgtgattt agttaggctt gatgacagga caactgagat atgtcgtaaa atcgctaaag
    18721 aaaacaaaag aaagctatta tctgagctaa agcctggaat taatgcagca ccctatcatt
    18781 tcaactgcag aacaaccatt attcctgatg aagatgagct aagcattgaa gtagatccaa
    18841 ttgatgataa aagcactaag tattttaagg atgtcacctc agattggata gatggaaatg
    18901 agcataaacc acaactgtca ctactaaatg agtatgtaaa aaatggcact ccttacaaag
    18961 tagatggcca taatgttgtt cttgatcact cgaattatga atatagagtt gctaattggt
    19021 tatctaaaaa gacaggattg caagttgaca tggtgccaag agttaattcc cctgaacata
    19081 tcaatactcc tgattattta gttgatggag ttccttttga tctaaaagaa atcacaggtt
    19141 cgggtaaaaa cgtcattgat ggcaatctac gaaaagcaaa aaagcaggca actaatatca
    19201 tttttgatat aacaaaaacc cctttatcat ttgaagagat aatggggcag ttagaacaca
    19261 tttatatgtt agatcgtagg ggtcttgata tatcaattat caaaaataaa gatgaggtat
    19321 tagctgtatt agaaaaagaa ggacgatgac ccaccgcctc cacagtaaac tgcttcatgg
    19381 gcgttagacc atcgtccttc tttatctcaa ttatacatca acctattaaa actatcaagg
    19441 aggaaaatat gtttatttgg caaatggtat tagtggcact aggtgtattg gtgctcatat
    19501 tgattgttgg cattgcagca atagcggtta agtcaattat tgcagaattg aaaaaggtag
    19561 gataaacatg aataagcgca ttaagaaaaa acgtaaattg gaaacagcgg ttgtgatgct
    19621 tgttgcagag aatgccatgc aagcagaagc tcttagaaat caaaacaaac aaattacaga
    19681 gctgagagcg attgtacaac aaaacgccca agctaccaat gaagagtttg cgtctgttaa
    19741 agctgctact ttagataacc aatcagctat tgcagatatt gcaattgacg ttaactatat
    19801 taagaaaaat tacaagcgaa aatggggaaa ataaatttta aacgaattcg acccctttag
    19861 aaatcaaagt cgtagcgatg cggcctttta ttatgtccaa actttgttga tgacactaaa
    19921 agctacactg tttcgtcgca ggacgtaaaa ctagattatc ggttggtggc gtaaccacta
    19981 ggagaaatta tggcagaaga aaatgtaaca acagaaacta ctgaacaagt cgacactcag
    20041 caagaaactg ttgaacatcc taagcaggaa catgagcgaa cttttactcg cgcagatatc
    20101 tcaaaaatga tgactgctga acgtgcaaag tgggaagcag agcaatcaga agcaatcgaa
    20161 aaagctcgta ctgagggtga aagactagct aaattatcaa aagatgaacg tgctaaagaa
    20221 gaagaacaaa aacgtctcag tgctattgca gaacgtgaaa aagcagttgc agaacgtgaa
    20281 atgcgcatcg aaacacaatc attacttgct gaaaaaggat tgccactaga ttttattgat
    20341 gttgttttag ctactactgc agatgaagtg aaaacaaaca ttgataattt acagtctatc
    20401 tttgatcaag cagtggaaaa tcgtgttaac gaaagattaa cgcaaaagtc acctcgaact
    20461 ggtaatggct ctgtcggtat gactaaagct gaaattatgg ctattgagaa tgatgatgaa
    20521 cgtctcaaag ctattgcagc taatcgtaat ttatttacaa ggtaagaaag gacatattaa
    20581 tttatggcag aaaataattt aaatgtgatg tcagacttag gagaaatcaa atctattgat
    20641 tttgttaata agttttcaac gaatatcaat gaactactca agcttttggg agtaactcgc
    20701 aaacaggaat tgactaatga tctaaaaatc caaacttata agtggtctac tactttagat
    20761 aagacaaatc ccgccgaggg tgaaacaatt cctttatcta aagttactcg tgcaaaagct
    20821 aaggaatata ctgttgagtg gttcaaaaaa cgtcgtgcaa ctaccgctga agcaattgca
    20881 cgtcatggtg tagctcgtgc gattaacgaa gcagatacac gcattatgcg agaaattcaa
    20941 aatggtttta aaactaactt ctttgaattt ttaaagaagt caactacaaa agtgactgga
    21001 acaactcttc aaagtgcatt atcacaatca tgggcaaaaa ttgctacttt taatgaattc
    21061 gatggttcac ctatcatctc tttcgtttct ccacttgatg ttgctaagta tctaggagat
    21121 acaaatgtag gtgcagatgc ttctaacgtg tttggaatga cattgcttaa aaacttccta
    21181 ggtatgcaaa acgttatcgt tttaccatca gtgccagagg gaaaagtata cacaacagct
    21241 gttgaaaacc ttgtgttcgc ttatttaaac gtcaatagtg gcgatcttgg agggatgttt
    21301 gctgatttta ctgatgaaac tggattgatt ggtgcggcta gaactcgtca actttctaat
    21361 ttgacctatg aatcagtatt ttttggtgca aatgtgctat ttgcagaaat tccagaaggc
    21421 gttgtagaag caacaattga ggctccaaaa gtagttccag gaggctaata gatggataag
    21481 gagttattac tcacagaggt taagctattc aaagggatca gcgccaaaga cactagccaa
    21541 gatgatttga taaaattatc cattaatgaa agtattgacc gtatccttgc gaaactcaat
    21601 gattactcag acacagaaat gactgaaatt cctaagcgtc ttacttttat tgtcagagat
    21661 gtggctatta aaaggtataa cagacttaat tcagaaggaa ccgttgaaga tagcgaagaa
    21721 ggaaagactt ttaagtggga cagttacctt aaagagtatg aatcaacact cagaagcgct
    21781 gctattggga aggtatattc aggcaaaggg gtagcaagat ttatttagga ggtcataaat
    21841 gatttacaaa gatagagtta tcctagtgta tgtcgatgag caggacgatt ttctagataa
    21901 aagaactgtt gaaaaaccta gcggaaaaat cccctgcatg gaaaatactt tcacaaaatc
    21961 tgaacagatg gggttatttg gtaaatatga tttgaacgct tttaagttgc acttgcaagg
    22021 ccgatacgat ggctttagca agattatcta caaggggaaa ccgaggttga taaagggact
    22081 agcgcaccat aaaaatagca cggttattta tgtatgagtc ttatttatcg gatgagaggc
    22141 ctagataggt ttttacgcag cgttgagcgc aagcagaagt cagtacgaat cgctgtagat
    22201 aaagagctta gtaaatcagc tgctaggatt gagagacagg ctaaaatact agccccggtt
    22261 gacactggat ggctgagagc tcaaatctac agtgagcaac aacgactctt acactacaga
    22321 gtggtttctc ctgctttata ttctatttat cttgagttag gtactcgtaa aatggaagcc
    22381 caaccatttt tagaccctgc cctgagaaaa gaatggccag tgttaatggc taatctcaaa
    22441 aaaatgttta agaggtgatg catggattac tcaccagaaa cactatattt aaaaaaggta
    22501 aaaaatagat tgggagtttt agacatacca atctatttta aattgcctaa atcagacgtt
    22561 ttagagcctt ttattgttgt aggtacaaat atatcagact tgtcaaaaac agctcaaact
    22621 ggagcagtta ttgatgattt tagcctgaat atcgatgctt tcttacctgg cgatagtcgt
    22681 ttggatgcag aagagataaa atctcgcatg cttagactgc ttgggcggaa taaccaaata
    22741 aaggctcaga ttttggtaga taattcaata ggacgagaag tctatagagt tgctatcaac
    22801 attacagaaa tactaattta aaggagaatt aaatggaaac tacagttgaa attactacag
    22861 ctaagcctat taaaggtaaa aatatatttt attttattca atctgttgat gctgaattag
    22921 gtagtaaagc aatactgcca gcgtatcgta cagatggaac aacgaagctc ggtggagatt
    22981 ttatcgatga acaaactcaa caaggacgtg tgcttgaaaa agcaacagat gagcactcaa
    23041 ttgatttgac atcatactac acgccgaaag acaaagcaat tgaaattgta gaaatagcta
    23101 aaacaactgg taaatctgtt aaagtttggc gtgttatcgc agacgaatct ttgaaagaaa
    23161 aaggttcagg aactttagag ggtaaagaag tttatcccgc gaagtttgga tatggaaaaa
    23221 ttgacgagat tgaatattca gaaggtgttg aaaaacttgt agaagcatca tatacattga
    23281 atattatcga tagtttaaaa gatggcaaat ttccactgac ggcagaagat atcgctttgt
    23341 taacaagtgt ttatgcatat caaaacccag gcgaaactac tggcgattat gacaatatcc
    23401 agaaaaaaga agtttcagga agtttaccgg gagcagttcc agggatatca tcagaaagac
    23461 actaatttat aaggtagcat ttcgctacct tttattttta aggagaaata cataaatgga
    23521 attaacagta gcaaaacgca gtattgaaat taaatttgat tttaaaacaa tgtttaagat
    23581 aaataacagg cttgggactg tgaataaaga gacaggggaa cgtaacgctg acggcgtcgg
    23641 tacgctattt tataaaatct tagagcgtga tgatacagct atagtagatt tagtcaagct
    23701 gtctgtaggt agcggtaaaa aagcattgtc agaagatgaa gtgcttgatt ctatcgcaga
    23761 gctagtcgaa gaagaaggat ctacagaagc tttatttaaa gaagttgaga atgacatggt
    23821 tgaatcaggt tttttcaaag agaagatttc gaaatatatc gaaagcatgg agaaatcagt
    23881 gaaatattta gaagctcgag aagatgcgga cgaagctcaa atccaaatcg taaaagacat
    23941 gattgggaaa atgaaagacg caatctctta attaactgtg cccaacttgg cctgacggac
    24001 attgatatta tttacaagtg tacgagaaca gaattagatg cgattatgga aggattgcag
    24061 tacaagcaaa ttgcagaacg tgaaaacctc tctgaacttg ctttaaatct cagatacacg
    24121 ttaaatgcta aaaacgtcaa tgctaacaag ctcaaattag ataaacaacg taacaaaatt
    24181 aaacgaactt atcaaaataa acaagatcta actaatattt caaacgattt cgcagaacgt
    24241 gtagcgaagc tcaatttaca ttttcaaaat ttataacaag atataacaag aaaggaggga
    24301 tttaatggca ggtacatttg atggggctat attcgctgat attggtgcaa atactaaaga
    24361 ttatgaggca gctatggcta gaattgttag tactacacaa aatgctttta gaaaagcgca
    24421 ggatgctgca gttaacaaca gcaacaaaat gatacagatg attggtcaaa ttatggctca
    24481 attagcaaat aatggtcaat cattagggca aagactcggt aatgcatata gtacaggtct
    24541 caagttatct cttggtcaaa ttcaaaggat agcagcatct atcggtgaaa aaattcccga
    24601 acccataaag aatgggttca ataaagcttt ttcaattatt caaaatagcg tacagaagtt
    24661 atcgagcgcc atccctcaac ccataaaatc cgcattcaca agtgcaacaa gcgcagtagt
    24721 tagcttttcg tctaagatca caagtgccgt ctcagctgct ttcaactctg ttagttctaa
    24781 agcgagtact gttgcagata aaatcagtag tagttttggg agtagaatca cttcagcggt
    24841 tactaattta gcgactaagt taagcacagg tcttagcaat ggttttagca ggatgtccag
    24901 ttctgcaact agtgccctaa atggtattag tcagagattc gcaagcgcat catctgctgg
    24961 tgaaaaacta agaagcactg tgggaagtat catttctgct tttagtttga tggccattgc
    25021 tcggaaagga atccaagcaa tcacaggagc aatggacggt gcaataagtc gtgtagatac
    25081 tatgaaccga tttccgaaaa cgatggcgct atttgggtat tctgcacaac agtctaaagc
    25141 atcaatagat aaattatcta aaggaattga aggacttcct acaccactag acagtgctgt
    25201 taaaagtgcc caacaattag ctataactac aggtagctta gataaaggaa cagagctagc
    25261 attagcattt aataatgcaa tgattggtta tggagcgact actgaagggg ctgagcaagc
    25321 acttagacag tttaaccagt cgttggggtc tggtaagata tatgccgaag agtttaattc
    25381 tgtgtctgaa gctgcccctg gcttaatgtc taaaatggca gagtcattcg gatttggtaa
    25441 aaatggtgtt caggacttaa aagcagctct ttcagacggc agtattactg cccaagaatt
    25501 cgctgataaa atgattgaac taaacaaggc gcagggtggt tttgctgaga tggcccaatc
    25561 atcggctggt ggaatccgaa ctgcatggaa gaacgtacat accgccgtcg taaaaggcgt
    25621 agcaggtatg atttcagcct ttgatgaagc tgccaaagct aatggtatga agaccattgc
    25681 agaatccatt aatagtatta agccagctat aaattctgca tttgcaactg tgaactcaat
    25741 aatgccaaac cttgttgcag cattcgctag aatctccaag atggtgaata ttgactttag
    25801 tccactaact agtggaatta aatcagcttt tggattatta aatactgctt taggtgaatt
    25861 tgcaaaaact ggtgaagtat cgtcagcaac tattgataga atcaaatcaa agatagcatc
    25921 tataggtcct actgcaatag cagcttgggc cttattaaat cctgcaagtg ctattgcaac
    25981 tattatgcct ttaatatcaa ttattggtaa gattggtgtt gctcttggga ctgttggttc
    26041 agcgttggga tcattcggag aaatggtagc cagtggattg tcaactgcta gtgggctagt
    26101 tggagctttt ggtagagcta tgtcaggact tcctggaata tttggtgtag ctgcaagtag
    26161 aggtcttgct gtacttgatg gaatgacaac tgcgattagt agtgctgcaa tactggcaat
    26221 gtctgctata ggtcctgcag caattttagg attggtcgta gtaggtcttg gtttagtaaa
    26281 caatcaattt ggtactcaaa ttgataaatt gataaataca gcaattacta aaggacctgg
    26341 tattattcaa ggccttgtaa aaggcattac ttctaaaata ccagctttga tagctagtgg
    26401 tactcaattg attgctaagt ttgctaatgc tctaacagta ttattgccag ttattattca
    26461 ggcaggtgtt caattaatta ccagtctggt gcaaggtatt ggtcaaaacg caggtagttt
    26521 aattagttcg gctcttaaag ttattggaac ttttgtaagt tcaattgcta gtgctctccc
    26581 tcaaatacta gctgtaggtg ttcaattaat actgaactta gctcgaggta ttgtacagaa
    26641 tataccttta ataatccagt atgctcaaca aattatttct aattttggtt caagcttaca
    26701 agctaatatg ccaagcataa ttagcaacgg tatagcaatt agcaccaatt tgaatgctgg
    26761 tattatccaa atgttgccgg cagttataca aattgcaacc caagtcatca caggttttat
    26821 ttccggtata gctcagtatt tgccacaggt aatccaaggc ggcatccaga ttataatcat
    26881 gttagtgcaa ggtattattc aaaatttacc gcagataatc caagcagcga tgcagattat
    26941 tcagtcattg atatctggtt tatcacaagc attaccacaa attatatctg cagggataca
    27001 gttagttgtg cagttggctg ttgcaattat taaagggttg ccgcagatta tttcagctgg
    27061 tattcaattg attgcgggtc ttggtaaagc tatgcttgag gctattccga atgctctttc
    27121 aggggtatgg gaaggcatta aaggtggatt tagttctatg tgggaccaaa tcacaggaaa
    27181 gagctcaaca agtgccgcta aagttacatc agacatgtca acaatgacta tgaatgttgg
    27241 tatgcagacg accacaatgg ctaatcaaac taacatagac acaactacta tgctaaatag
    27301 cattagtcaa aatactggtc ttgctaattt aaatgcgaca acaaatgcta ctcagatggc
    27361 aacaaatgta aatgcacaaa cagcatctat ggcaaatcaa ggtttgctga atgcggcagg
    27421 atttaatgaa gcgttgagtt cgaacatgac tttagcaaat acaaatgcta ctaatcaagc
    27481 taatcaaatg actgctaatg ttaataacgc tactagccaa atgagtctgg ggtctctacc
    27541 gaatttcttg gaattatcgc aacaggcaac aagtaatatg caaactgcac aagcaaatgc
    27601 taccacttcc gcgaatggaa tgagttctgg agttactggt gctacagctg gtatggctag
    27661 tggtgtagct aataacatgt caggtataac tagcactgta tctacaggga tgaatcaagc
    27721 tgcaaatgct gcaaaatcag gcggtgcaaa tatatctaac agtgtgaata gcagttttac
    27781 acaagctaaa tctacagttt caagttcaat gtcaggaatg aacagcactg ttactagtgg
    27841 aatgaatagt attaataata ctgtaaaatc aggtggaaat aatattaaat ccacctttac
    27901 aagttccttt aattctgcta agtctacagt ttcaaatgct atgtctagta ttcgttcgtc
    27961 aatttcaagt ggaatgaaca gcgctgtgag tgtaacaaga agtgcaatga attcggttgt
    28021 atctgcaatg aatgcagcag cgagtggtgc tagatctgca ggttataata ctgggattgg
    28081 atttgcaaat ggcctagcaa gcactgcagg aactattatg gctattgcta atagtattgc
    28141 tgctaatgtc gctgcaacaa tcagaagcgc aatggatatt cattcgcctt cgcgagtaac
    28201 gcgagaactt ggtgcttata ctggtgaagg ttttgcactt ggtatgcagg attggatgga
    28261 tgatatcaat gcaataggta aagagtacgc taatgctgtg actgatcagc gatgggaaac
    28321 agattcacaa ttagcgacat ctgcaaacat atcaagtgca ggtgtatcat catcacttga
    28381 taatctaagc gacgaggtac gcaaccaaca attgactagt ccaacgttcg aggttcataa
    28441 tgaacttgtt ggtgatgaaa tttatacaac tattaaatct aaagaagcaa gagaaagtaa
    28501 taaagataat tacttcaact ttgcgacata agagaggaga aaaatggatt tattagtaca
    28561 aaagagtgat caatcctttc gattttcaga gattggtcta cgaatacttg atgtcgatga
    28621 ccgttcatca tctcttgagg ttgatagtcg aacagtgaaa ggtcgaagcg gtaaaatctt
    28681 tgctagtgct aagtatggca caaaaaaagt taaggttaat gggcgcttag ttgtagcttc
    28741 tattaaagat tttatgacaa aaaaagatga tgtgaatggc ttactagtgg atagtgagcc
    28801 tttttacatt actaaaatgt atcctcagaa agaaaattta tatgatttcg aattgcctgg
    28861 gatgaaagca ggtgatttag acttattaaa tcaatctcat acagcgtggc actatcgttg
    28921 gaaagtttat gtctcaagtg aacctgagta cacttttgtt ggtaagtcta gtgaaggtct
    28981 caaatacaac tttacaattg catttgagac tgcagaactt ccatttggtg aaactattgc
    29041 acaaaatata gtcattacta acggaacgtt taattattct ggtactgcac cttttagcca
    29101 gttagaatac ccatggtctg tagagttaac ttcaactggt ggacaaagta gtttttatct
    29161 tgaaatcgat gggaggcgct gggcttatca atatagtgca ccacttaatc aaggcgacgt
    29221 tattgtaatc actggtatat ccacaacatt gaataagata aatgtgactg ctagaaccaa
    29281 tcatgaatat tttgttttaa agcaaaaagt caacaagact attctctaca gcacaaattt
    29341 tcaaggcaca atcaagtttt taaactttaa agaactttac aagtaaggaa gtgatagatt
    29401 gacaactttt ttagatcata aagataatga gtttgaagct caagctgttg ttaaaacaac
    29461 taatgcagtt aacggagaaa ggtctttatc aggaacaatt tatactaacg aagaggtact
    29521 aaacaaggtc gataaaggtt ggaaacttga gtttgatgat gagtattaca agattattta
    29581 tgctaaacct actgatactg gtaacaaaat tcaagttgaa tttgatgcag ttcatcaatt
    29641 tttttatgat tttgataaat catcactaca taagcaatta aatgatggtt cacatacttt
    29701 ccaagcatat ttgaacttta tttttaatgg cagcgggtat acttacagtt tagaagtagt
    29761 tgtcaaatct ttcgaaaagc aatcgtttgg atataaatcg cgacttgatt tatttaatga
    29821 catcattaag agtgcaggag ttgaattttt tgttcgtggc aaggttgttc gaattctaca
    29881 aaaaacagga acagatttat caacaatcgt acgtaaaaat ttcaatatga atgaaatcat
    29941 aattgaaaag aaaatcaatg attttattac atatcagaag ggttttggag cgtggactga
    30001 ccaagaggat cattccaaag gaaggctcga agtagaatat gaaagtccac ttgcaaagga
    30061 atatggacga attgagggtg agcctgttgt tgatgagcgg tacactaacg ctgacaattt
    30121 aaaatcagta ctaaaacaaa atgtagattc gtcttacaat atcgctgtaa aaattgacat
    30181 tgaagatctt acaaaagcag gttatcagta cactcaacct attgccggtg attatattat
    30241 ggctatcaat gagacactag gatttaagga aaaaatccga atagtatcgt ttgaaagtga
    30301 gtatgatgta acaggtcagc tgattaacca taaagtcatt tgtaacgata ttggtacaat
    30361 caaaaaacaa actgtagctg ttagccaatt atcaagtaag attaataata accaagaaat
    30421 actagataat gcagtattta aagcaaatca agcactagca tcagctgatg gtaaaaacac
    30481 taactactat ggtacggaga tgccagtaga tgatccaaaa ggcacgctaa gaaagggtga
    30541 tttgcttttt ttaactgttg gcgatactac cagaatgtat ttctggaatg gtgctgaatg
    30601 gattatcaat tcttttagcg acgatattga ttttgtaaag aaagaaatta ctaataaaat
    30661 catagaggtt aatgcagcaa tgaaacttgc tgatgaacag ttaactgaaa aatataatgc
    30721 aattgttgct aaaaatgtta gccaagacga attaattaat caagcaaaga atttgtctga
    30781 ttctgccaaa aaagatgcta cagaagcatt gacaaacctt attagtgaat ctaaaaaact
    30841 gacggataaa atgtctgcgt tatctaaaac agaagttgag cattattcaa caactaatct
    30901 gcaactgaca caacttgatg gtacagtcaa aggattacaa actagctacg aggcattgtt
    30961 aaaaaaagat ggagatatca ctcaaacact cgctaattat aagcagacga ttgatcaaaa
    31021 tagtacaagt attactgcta acaaaaagac tgttgatggt acgcttagca gtctacagac
    31081 ccaagtcaca caaacggcta atgaaattac aacgagatta tctcaaacaa atattgaagc
    31141 gttgattaca agtaagtctg ctgttatcgc tgataataaa gtcaaggaaa ctgcagatag
    31201 ttttagcaga gaaataactc gtgttaataa tactattgat aatttaaaga taggtacagt
    31261 taatctacta tctggtacta aaaattggac taaaaattgg tttaatcgta ataactggca
    31321 atctgatgac gataacttga ctattggtaa ttttagctta acagtgctaa agcgcaaagc
    31381 aatgtggagt ggtatatcac aattgtatgc tgtaaaaagc ggagagaatt acgtttttag
    31441 tgcatacgct aaatcgagca tggataatga cgttgtgaat ttatatttat taccaaacgg
    31501 tcaatcgccg caagcaaaga taagtattag tttcaagtca atcactctaa acacggattg
    31561 gcaacgtatc gttattaaat ttaatgtaac ttctgatggc tatatcttac cgcgttttga
    31621 acgttttaac gaaaacggat ggttgtatat tgcaggctat cagctagaac atggaaacgt
    31681 tgcaagtgac tattcagagg caccagccga tcaagaagac aaaatgactg ttgaatttaa
    31741 taaaatcaac gatacagtta acagccatag tcaattgatt ggtaaacaga acgagagtct
    31801 gacggctaca atacaaaaag ttgataatat acaatcaaca gtatctaatg ttgatggacg
    31861 tttgtcacga gttacacaaa ctgccgacgg tatagtcaca acagttagtc agcttgataa
    31921 taatatttta cctggcacaa aaaattttac aggatggcaa atagaaggtg cagtactaga
    31981 agatgtaaca caatcatctt atccatttgt ctttaaaaaa tggataagcg gcaacaaggt
    32041 atcgccctta atcgaatatg atgttaaaaa aaatcaagag tacacattta ctgcatacat
    32101 tgctagagaa caagcaggta atctatattt ctacttgtac gatctttgga atcatcatat
    32161 cacaagtgca acccctcgcg aaaccattat aagagacgtt aattctagtg taatacgatt
    32221 tagaataaca tttattccaa atagagacgg taaaatcaga ccacggttcg caatgttggc
    32281 cagtgatcaa ggctggttta tggttggtgg ttttatgtta tctaaaggta ccgctgatct
    32341 accctggtct gaatccaagc aagatatcaa agataatgtt actgcgatta atacaatagt
    32401 cgcacaaaca gcgaacagtt gggctattaa aaatttgact tctaacggta ctgtattgag
    32461 tcagcttaat ttgactgatg gtaacgtaaa actggaaggt aagttaatac atttgtcagg
    32521 tcaatctatt attgataatg gcattattac taatgcgatg ataaaagatt taactgcaga
    32581 taagattaca gcaggaacaa ttgatgctaa tcgtataaat gtcattaatt tagatgctag
    32641 taatattacc agtggaagaa tgtcagcgga cctgatacgt agcggcgttt taatatcgca
    32701 aaacggtgca atgttaaccg atcttaatac aggacaaatt gaattttaca cagataatcc
    32761 agctatcaaa cgtattactg caggttatcc taaccaattc gttaaatttg caacaggtaa
    32821 tgttgaagga aagggcgcgg ctggcgtaac ggttataggt tctaatcgat ggggaagtga
    32881 atcgtcaaac gacggtggct tcgttggtat tcgcgcttgg aatggtaaag acatcgatac
    32941 tcttgattta gttggcgata gtattcactt agcaagctct gcgtacacta acgctgatgg
    33001 ttgggaaatc attactttgc caaatcagct atcaatagat gcacgtaaca taaatcatcg
    33061 tgtaacgtct aaggtaaaag taggagatat ttggctatgg aaaaatacat caacttatgt
    33121 aagcatgaaa gatacaatta atatgattat tgataacttg cagctactac acaataataa
    33181 aaccactgaa aaaggctaca gttatactat tccaggaaag atttaggaga aaaaatggaa
    33241 gaaaaaataa aatcaaattt agctttacaa atagcgcaat tatctttaga taaagcaact
    33301 ctacaagctc aaaatgagca attacaatta caaattgctg agttaaatca gcaattagat
    33361 gagataacta agccaggagc aaagtgaggt gagtaacatg aaacaatgga acattgttgg
    33421 aaaaccattt ccaatctatc aagatggagt ggttctaaaa acggatgtac gcttacaagg
    33481 cgacgatggt acatatttac cacaagagct aattggtaat catgttgata aggataatca
    33541 atcactgata aaacttgtac ttgaaacttt tgcaaaagaa aatgtcgttg atttcgcaat
    33601 gcttgagtct gtaaaagata ttgagcaact aaaagctgac aaagtaactt taatcaaaaa
    33661 aattgcagag gctgatgagg caatttctaa gatgtcgcaa gcaacaaaag atatgcagct
    33721 taaagttgat aaagcagttg tcgagttaac tgacttagtg acatcaagtg tatctagcct
    33781 ttccgtgccg tctacatcga tggaatcaag ctaatggaac taacgttgaa taccgttata
    33841 acactatatt atttaataaa aaaaggagaa tttaatatga catatacttt taaaaaagat
    33901 catcttattg ttgacgtttg gtatcgccgt gttgcgaatg gtatttgtga tttcgaagca
    33961 gtacctaaat tatttaattt acgagatatt gtgcttgagt tacttagtca aaaagttact
    34021 gataaagaat cagagtgagg taacttaatg atagattttg tgcaagttgg cgcttttgca
    34081 ggtgctgctt tatctatttt gggagtatgg ggatttattg ttaatccctt taaaaaagca
    34141 atggaagcta acgagattgc tatggctcag ctcaaggact cactcaaaga actagcatac
    34201 gagctcaaaa atatagatag agaccgagag attacaaaag ctgttattga tcgtcacgag
    34261 gagcgtttag gtcgtgtcga ggatgatatc atcatcaata aagagcaaat caaaacatta
    34321 tttaataagg agaaataaca tgattaattg gaaagtaaga attaaaaaca aagcattttg
    34381 gtcagcaatt attccagcaa tatttttagt tgtacaagca gttgcaaatg tttttggtta
    34441 tacacttgaa cttagtgatt taggtaataa attgttagta gttgttaata gtgtgttttc
    34501 agtacttgtt attgcaggta ttgttacaga ccctactaca caaggagtat cagatagcag
    34561 ccgtgcgcta tcctacacag agccaaaata ataggaggat aaaatgaaag caatcacgaa
    34621 aatagcatta gtactagtaa tagcaatatt atatattccg ttgtcagtga ttgctttttt
    34681 tatttatccg ttttttgata aggaggacag atgacctttt tagataacat taagcaaggc
    34741 tgcttagatg gctggactaa gtacaaaatc ttgccatcgc taaccgcagc acaggcaatc
    34801 ctagagagtg gttggggtaa atatgcacca cacaacgctc tatttggtat caaagcagat
    34861 agctcttgga ctggtaagtc gtttaacact aaaacccaag aggaatatca agctggtgtc
    34921 atgacggata ttgtcgacag gtttagagcc tatgacagct ggactgacag cattgtcgac
    34981 cacggtaaat ttttaaacga taatccacgc tacaaagctg tgattggaga gactgattat
    35041 aaaaaagctt gttatgctat taaagacgct ggttatgcta cagcaagcgg atatgcggag
    35101 ttgttgatac aactaattga ggaaaataac ttgcagtctt gggatgccga aactataaaa
    35161 aataaggagg aaacgatgac aaccgcaaac gaaattgtac aatactgtgt taaccttgcc
    35221 aactcgggca tgggagttga taaagatggt gcttatggga cgcagtgtgc tgacttacca
    35281 tgttttatcg tcaaaaactg gtttggcatt gatttatggg gcaatgcgat tgacctgtta
    35341 aatagtgcag cagcacaagg attagaggtt atctatgatg cccccggagt caatcccaaa
    35401 gccagtgact tatttgtcat ggaggtagct ggaagtccgt acggacatac aggagctgtc
    35461 atcgaggaca gtgacggcta tactattaaa accgttgaac aaaacattga cggcaactgg
    35521 gatagtcttc aagtaggtgg ccccgctcgc tttaatactc gtgactttac tggcgttgtc
    35581 ggctggatta ggttacctgt tgaccatact aaccagacag tagacacatc accacaaacc
    35641 tcagatactc tcgtagagac gccaaaatct ggtaccttta tgcttgatgt tgcggagatt
    35701 aatattagac gttggccaag tttagccagt gaagtagtag gcagctataa gcaaggtgac
    35761 aacgttacct ttgatagtga ggggtacgcc aatggttact attggattag ctatgttggt
    35821 agctccggta agcgtaatta catggctatt gggcaaacgg ataaagacgg caagcgcatc
    35881 agcttgtggg gtaaaataaa ataaagcatg tgattaacag agcgacataa atgtcggtca
    35941 gttaatggtg taacttacac cgcaactaaa aaaacaattt aggaggtaaa actcctttag
    36001 ataagacaaa tgccctcgct tttgcggggg ctgtttttgt gtcttaaaga tttcatgttt
    36061 gacgaatgta gtatattatg ggataatagt aatgaacatg aacggtgaaa aacctccttt
    36121 ctatgttccg acctgttcat gtcgtaaaac tccatgcttt ttggcaatga gggggcggag
    36181 ggacgcgctc gttaacataa gtatcccgtt ggcaatgcgt cctaccaata cccagttggt
    36241 aggatttttt aatttttaga aggcaaataa tgaagagtaa acaattagta ttagggcaga
    36301 ttgatttgaa tatgtgtcgg gatttcaatc tggcacaagc catggattat aattccaaga
    36361 ctaaaagact atataataaa gggcgaggtt ttgctattgt gttggtaact attcaaaatc
    36421 taacttttgc aatccctttg agaagcaata ttcctaaaaa atatcaacta aaatataaat
    36481 taagagactc taaaaaatac ggatgtgtag agggacttga tattggcaaa gctctagtag
    36541 tagaagatcc aaaatatatt ctaaacagaa cgttcaaatt acgagagcag gttgactatt
    36601 ttaagattgt tgataatgat atacttattg ttaacaagtt gataaaggct atcatagatt
    36661 ataatcaagc tgtggctaat agcgaccaaa ataaattaac agatcctaaa cgtttcaaat
    36721 tttctacttt tccaaattat actgatcgac ttaaaagtat tacagccgct gattacctct
    36781 attgacatac tcgcataacc atgagataat cacagtagca agaatcgcct gacactagcg
    36841 gttcttgctt ttttatttgc ctagaaataa tcaaaatgtt accatagaat aaaaataata
    36901 aggaggcaca ttatgtcaca agaaaaacta aaatcaaaat tagatcaagc aaaaggtggt
    36961 gctaaagaag gctttggcaa aataaccggt gataaagagt tagaggcaaa aggttttgtt
    37021 gaaaaaacaa ttgctaaagg caaagaacta gcagatgatg ctaaagatgc tgttgaagag
    37081 gcagtagatg ctgtcaaaga aaaactgaaa taaatattaa ccgctctcta ttgagggcgg
    37141 tttttttgtg tgtctagagt ttgctttcaa ttaattgttt taattctaat aagtcttctt
    37201 ttgtagcatt tttgttaata aaactacgag cagtagatcg ttttgataga taggttctat
    37261 gttctctatt gttttctgcc cactt
//
LOCUS       DATRMA010000012        36560 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.012, whole genome shotgun sequence.
ACCESSION   DATRMA010000012 DATRMA010000000
VERSION     DATRMA010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 36560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36560
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.012"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            complement(76..1101)
                     /locus_tag="VBC28_000567"
     CDS             complement(76..1101)
                     /locus_tag="VBC28_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEO6814569.1"
                     /translation="MLHFENDYNKGAHPELLNALIETNDQGLSGYGTDSYCQQAADKI
                     REVCSCPQAEVEFLVGGTQTNQVVISSMLASYEGVIAAETGHVSSHEAGAIEFSGHKV
                     LTLPSHNGKLLASEVATYIETFYADGNYQHMVFPGMVYISHPTEYGTLYSKAELEELS
                     KICKHYQIPLFIDGARLGYGLAAKDTDVDFPTIAALSDVFYIGGTKMGALAGEAVVFT
                     KKNRPKQFTTIVKQHGALLAKGRLLGLAFDRFFTDNLYLKIGKHAIDLAEELKIILEE
                     KGYSFYLKSPTNQQFIIVENTKLADLAKNVAYSFWEKYDDHHTVIRLATSWSTSREDV
                     TALRNVL"
     gene            complement(1221..2231)
                     /gene="tsaD"
                     /locus_tag="VBC28_000568"
     CDS             complement(1221..2231)
                     /gene="tsaD"
                     /locus_tag="VBC28_000568"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEO6814570.1"
                     /translation="MKDRYILAVESSCDETSVAILKNDKELLANIIASQVESHKRFGG
                     VVPEVASRHHVEVVTTCFEDALQEAGIVASDLDAVAVTYGPGLVGALLVGMAAAKAFA
                     WANKLPLIPINHMAGHLMAARDVKELQYPLLALLVSGGHTELVYVSEPGDYKIVGETR
                     DDAVGEAYDKVGRVMGLTYPAGREIDQLAHKGQDTYHFPRAMIKEDHLEFSFSGLKSA
                     FINLHHNAEQKGEALVLEDLCASFQAAVLDILLAKTQKALLKYPVKTLVVAGGVAANQ
                     GLRERLATDISPDIDVVIPPLRLCGDNAGMIALAAAIEFEKENFASLKLNAKPSLAFE
                     SL"
     gene            complement(2307..2714)
                     /gene="rimI"
                     /locus_tag="VBC28_000569"
     CDS             complement(2307..2714)
                     /gene="rimI"
                     /locus_tag="VBC28_000569"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEO6814571.1"
                     /translation="MESSEQALAIWSVLSDVYDKSPWSLSQISEDLKKDSTDYFFVYN
                     DGEVIGFLALQQLVGEVEITNIAVKKNYQGKGYAYQLMSMIADIEVPVFLEVRYSNIV
                     AQKLYERCGFVVLRKRKNYYHDPIEDAIVMRKG"
     gene            complement(2716..3408)
                     /gene="tsaB"
                     /locus_tag="VBC28_000570"
     CDS             complement(2716..3408)
                     /gene="tsaB"
                     /locus_tag="VBC28_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEO6814572.1"
                     /translation="MMKVLAFDTSSKALSVAVLNNMECLATVTINIKKNHSINLMPAI
                     DFLMQSIDLEPQDLDRIVVAEGPGSYTGLRVAVATAKMLAYTLKIDLVGVSSLYALTN
                     GFSENDLLVPLIDARRNNVYVGFYQNGDTVKPDCHTSLEEVLQEVGNKANVHFVGEVA
                     AFFDQIKKALPHAKITETLPCAVAIGRKGQKMKSVNVDAFVPRYLKRVEAEENWLKNH
                     CETNTEEYIKRV"
     gene            3587..3817
                     /locus_tag="VBC28_000571"
     CDS             3587..3817
                     /locus_tag="VBC28_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HEO6814573.1"
                     /translation="MIYKVFYQETKERNPRREQTKTLYVTIDAANELEGRIAARKLVE
                     ENTAYNIEFIELLSDKHLEYEKETGVFELTEF"
     gene            3871..5550
                     /locus_tag="VBC28_000572"
     CDS             3871..5550
                     /locus_tag="VBC28_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEO6814574.1"
                     /translation="MSNINLKPEEVGVYAIGGLGEIGKNTYGIEYQDEIIIVDAGIKF
                     PEDDLLGIDYVIPDYSYIVENIDRIKALVITHGHEDHIGGIPFLLKQANLPIYAGPLA
                     LALIKGKLEEHGLLRDATLYEIHANTELTFKNLSVTFFRTTHSIPEPLGIVIHTPQGK
                     VICTGDFKFDFTPVGEPADLHRMAALGEDGVLCLLSDSTNAEVPTFTNSEKIVGQSIM
                     KIIEGIEGRIIFASFASNIFRLQQAAEAAVKTGRKIAVFGRSMEKAIVNGIELGYIKV
                     PKGTFIEPSELKNLHASEVLIMCTGSQGESMAALARIANGTHRQVTLQPGDTVIFSSS
                     PIPGNTTSVNKLINTIQEAGVDVIHGKINNIHTSGHGGQQEQKLMLRLIKPKYFMPVH
                     GEYRMQKVHAGLAVDTGIPKENIFIMENGDVLALTSDSARIAGHFNAQDIYVDGNGIG
                     DIGAAVLRDRHDLSEDGVVLAVATVDFDSKMILAGPDILSRGFIYMRESGDLIRESQH
                     ILFNAIRIALKNKDASIQSVNGAIVNALRPFLYEKTEREPIIIPMVLTPDK"
     gene            complement(5708..6217)
                     /locus_tag="VBC28_000573"
     CDS             complement(5708..6217)
                     /locus_tag="VBC28_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEO6814575.1"
                     /translation="MEENMNIKQLKSKTMLGTVALVSAFSFASTNADANTYNYAVDVD
                     YLASAEEIAQAHPASNTFPLGQCTWGVKEMATWAGNWWGNGGDWAASAASAGYTVGTQ
                     PRVGSIVCWTDGSYGHVAYVTAVDPVTNKIQVLESNYAGHQWIDNYRGWFDPQNTVTP
                     GVVSYIYPN"
     gene            complement(6365..7711)
                     /gene="glnA"
                     /locus_tag="VBC28_000574"
     CDS             complement(6365..7711)
                     /gene="glnA"
                     /locus_tag="VBC28_000574"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009731741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEO6814576.1"
                     /translation="MTITAEDIRREVKEKNVTFLRLMFTDILGVMKNVEIPATDEQLD
                     KVLSNKAMFDGSSIEGFVRINESDMYLYPDLDTWIVFPWGDENGAVAGLICDIYTAEG
                     EPFAGDPRGNLKRNMKRMQEIGYKSFNLGPEPEFFLFKMDENGNPTLDVNDKGGYFDL
                     APTDLADNTRREIVNVLTQMGFEVEASHHEVAVGQHEIDFKYDDVLKACDNIQLFKLV
                     VKTIARKHGLYATFMAKPKFGINGSGMHCNMSLFDNEGNNAFFDPEDPRGMQLSEDAY
                     YFLGGLMKHAYNYTAIINPTVNSYKRLVPGYEAPVYVAWAGRNRSPLIRVPASRGMGT
                     RLELRSVDPTANPYLALSVLLGSGLEGIENKIEAPEPIETNIYAMTVEERRQAGIVDL
                     PSTLHNALKALEEDEVVKAALGTHIYTNFLDAKRIEWASYATYVSQWEIDNYLDLY"
     gene            complement(7745..8116)
                     /locus_tag="VBC28_000575"
     CDS             complement(7745..8116)
                     /locus_tag="VBC28_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEO6814577.1"
                     /translation="MKERELRRTMAVFPIGAVMKLTDLTARQIRYYEDQGLITPERTE
                     GNRRMFSLNDMDRLLEIKDFISDGLHISDIKNEYMQRQHKSKEKQKSLSDAEVRRLLQ
                     DELRNQGRFSSPSQHIGNMHL"
     gene            complement(8196..8735)
                     /locus_tag="VBC28_000576"
     CDS             complement(8196..8735)
                     /locus_tag="VBC28_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000854122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEO6814578.1"
                     /translation="MKTLKKLLSNYKFDIKKFKLGMRTFKTGLSVFLVLLVFHLFGWK
                     GLQIGALTAVFSLREDFDKSVHFGFSRIIGNSIGGLLSLVFFAFNEIFHQAFWVTLLI
                     VPICTMLCIMINVACNNKSGIIGGTAALLIITLSIPSGETILYVFARIFETFCGVFIA
                     MMVNTDIEILRKKLKNNKL"
     gene            complement(8998..10194)
                     /locus_tag="VBC28_000577"
     CDS             complement(8998..10194)
                     /locus_tag="VBC28_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEO6814579.1"
                     /translation="MAKLTVKDVDLKGKKVLVRVDFNVPLKDGVITNDNRITAALPTI
                     KYIIEQGGRAILFSHLGRVKEEADKEGKSLAPVAADLAAKLGQDVVFPGVTRGAKLEE
                     AINALEDGQVLLVENTRFEDVDGKKESKNDEELGKYWASLGDGIFVNDAFGTAHRAHA
                     SNVGISANVEKAVAGFLLENEIAYIQEAVETPERPFVAILGGSKVSDKIGVIENLLEK
                     ADKVLIGGGMTYTFYKAQGIEIGNSLVEEDKLDVAKDLLEKSNGKLILPVDSKEANAF
                     AGYTEVRDTEGEAVSEGFLGLDIGPKSIAKFDEALTGAKTVVWNGPMGVFENPDFQAG
                     TIGVMDAIVKQPGVKSIIGGGDSAAAAINLGRADKFSWISTGGGASMELLEGKVLPGL
                     AALTEK"
     gene            complement(10329..11198)
                     /locus_tag="VBC28_000578"
     CDS             complement(10329..11198)
                     /locus_tag="VBC28_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase, lipoprotein e(P4) family"
                     /protein_id="HEO6814580.1"
                     /translation="MKTTKVFSVLSLSVAVFMMTACQSNHSTKKADMSPDKVEQKTKF
                     KNISLSSNKLLAKENTMSVLWYQNSAEAKALYLQGYNVAKMKLDDWLQKPSEKPYSII
                     LDLDETVLDNSPYQAKNIKDGSSFTPESWDKWVQKKSAKAVAGAKEFLKYANEKGIKI
                     YYVSDRTDAQVDATKENLEKEGIPVQGKDHLLFLKKGMKSKESRRQAVQKDTNLIMLF
                     GDNLVDFADFSKSSSTDREQLLTKLQSEFGSKFIVFPNPMYGSWESAIYQGKHLDVQK
                     QLKERQKMLHSYD"
     gene            complement(11407..12417)
                     /gene="gap"
                     /locus_tag="VBC28_000579"
     CDS             complement(11407..12417)
                     /gene="gap"
                     /locus_tag="VBC28_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEO6814581.1"
                     /translation="MVVKVGINGFGRIGRLAFRRIQNVEGVEVTRINDLTDPNMLAHL
                     LKYDTTQGRFDGTVEVKEGGFEVNGQFVKVSAEREPANIDWATDGVEIVLEATGFFAS
                     KEKAEQHIHENGAKKVVITAPGGNDVKTVVFNTNHDILDGTETVISGASCTTNCLAPM
                     AKALQDNFGVKQGLMTTIHAYTGDQMILDGPHRGGDLRRARAGAANIVPNSTGAAKAI
                     GLVIPELNGKLDGAAQRVPVPTGSVTELVATLEKDVTVEEVNAAMKAAANDSYGYTED
                     PIVSSDIVGISYGSLFDATQTKVQTVDGNQLVKVVSWYDNEMSYTSQLVRTLEYFAKI
                     AK"
     gene            complement(12621..14699)
                     /gene="fusA"
                     /locus_tag="VBC28_000580"
     CDS             complement(12621..14699)
                     /gene="fusA"
                     /locus_tag="VBC28_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEO6814582.1"
                     /translation="MAREFSLEKTRNIGIMAHVDAGKTTTTERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWDGHRVNIIDTPGHVDFTIEVQRSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATEYGVPRIVFANKMDKIGADFLYSVQSLHDRLQANAH
                     PIQLPIGSEDDFRGIIDLIKMKAEIYTNDLGTDILEEDIPAEYVDQANEYREKLVEAV
                     ADTDEDLMMKYLEGEEITNEELMAAIRKATINVEFYPVLCGSAFKNKGVQLMLDAVID
                     YLPSPLDIPAIKGINPDTDEEETRPASDEEPFAALAFKIMTDPFVGRLTFFRVYSGVL
                     NSGSYVLNTSKGKRERIGRILQMHANSRQEIETVYAGDIAAAVGLKDTTTGDSLTDEK
                     SKVILESIEVPEPVIQLMVEPKSKADQDKMGIALQKLAEEDPTFRVETNVETGETVIS
                     GMGELHLDVLVDRMKREFKVEANVGAPQVSYRETFRASTQARGFFKRQSGGKGQFGDV
                     WIEFTPNEEGKGFEFENAIVGGVVPREFIPAVEKGLVESMANGVLAGYPMVDVKAKLY
                     DGSYHDVDSSETAFKIAASLALKEAAKSAQPAILEPMMLVTITAPEDNLGDVMGHVTA
                     RRGRVDGMEARGNTQVVRAFVPLAEMFGYATVLRSATQGRGTFMMVFDHYEDVPKSVQ
                     EEIIKKNSGE"
     gene            complement(14854..15324)
                     /gene="rpsG"
                     /locus_tag="VBC28_000581"
     CDS             complement(14854..15324)
                     /gene="rpsG"
                     /locus_tag="VBC28_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEO6814583.1"
                     /translation="MSRKNQAPKREVLPDPLYNSKIVTRLINRVMLDGKRGTAATIVY
                     DAFEAIKEATGTDALEVFETAMENIMPVLEVRARRVGGSNYQVPVEVRPERRTTLGLR
                     WLVNASRARGEHTMKDRLAKEIMDAANNTGASVKKREDTHKMAEANRAFAHFRW"
     gene            complement(15346..15759)
                     /gene="rpsL"
                     /locus_tag="VBC28_000582"
     CDS             complement(15346..15759)
                     /gene="rpsL"
                     /locus_tag="VBC28_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEO6814584.1"
                     /translation="MPTINQLVRKPRKSKVEKSDSPALNIGYNSHRKVHTKLSAPQKR
                     GVATRVGTMTPKKPNSALRKFARVRLSNLIEVTAYIPGIGHNLQEHSVVLIRGGRVKD
                     LPGVRYHIVRGALDTAGVADRKQGRSKYGAKRPKG"
     gene            complement(15978..16790)
                     /gene="purR"
                     /locus_tag="VBC28_000583"
     CDS             complement(15978..16790)
                     /gene="purR"
                     /locus_tag="VBC28_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HEO6814585.1"
                     /translation="MKLRRSERMVVISNYLINNPYTLTSLNTFASKYGAAKSSISEDI
                     AIIKKAFEQAQIGDIKTVTGASGGVIFTPTIAEAEAKEIVEELRQRLSENDRILPGGY
                     IYLSDLLSTPKMLQSIGRIIANAYRGQKIDAVMTVATKGVPLANAVANVLDVPFVIVR
                     RDLKITEGSTVSVNYASGSSGRIEKMFLSKRSLKPNSRVLIVDDFLKGGGTVSGMISL
                     LSEFDSTLVGVAVFAENAQEQREKMAYKSLLRVSEIDVKNNRVSVEAGNIFD"
     gene            complement(16887..17828)
                     /locus_tag="VBC28_000584"
     CDS             complement(16887..17828)
                     /locus_tag="VBC28_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease YhaM family protein"
                     /protein_id="HEO6814586.1"
                     /translation="MKINQMKKDELFEGFYLIKKAEVRKTRAGKDFIAFTFQDDTGEI
                     SGNMWDAQTYNVEEFVAGKIVHMKGRREVYNGTPQVNQITLRNIKDGEPNDPRDFKEK
                     PPINVDNVREYMEQMLFKIENATWQRVVRALYRKYNKEFFTYPAAKTNHHAFESGLAY
                     HTATMVRLADSIGDIYPELNKSLMFAGIMLHDLAKVIELSGPDNTEYTIRGNLIGHIS
                     LIDEELTKILAELNIDDTKEEVTVLRHVILSHHGQLEYGSPVRPRIMEAEIIHMIDNI
                     DANMMMMTTALNRVNEGEMTNRIFAMDNRSFYKPNIK"
     gene            complement(17818..19092)
                     /locus_tag="VBC28_000585"
     CDS             complement(17818..19092)
                     /locus_tag="VBC28_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000960179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEO6814587.1"
                     /translation="MLVIILIIVLASLTVTIISYQKMTELTKSVEKQLEDNADNLSDQ
                     LTYQIEVAQKDQILTLTNQLNRMQQEIYQLLTDMRTELNQHLTESRDRSDKRLELINS
                     NLSQSVQKMQDSNEKRLDQMRQTVEEKLEKTLQTRLQTSFETVSRQLESVNQGLGEMK
                     TVAQDVGTLNKVLSNTKTRGILGELQLGQIIEDIMTVSQYEREFPTVSGSSERVEYAI
                     KLPGNGQGDYIYLPIDSKFPLEDYYRLEDAYELGDKVQIELYRKSLLASIRKFAKDIN
                     NKYLNPPETTNFGIMFLPTEGLYSEVVRNATFFDSLRRDENIVVAGPSTLSALLNSLS
                     VGFKTLNIQKNANDISKILGNVKVEFGKFGGMLSKAQKQLNTASKSIDSLLTTRTNAI
                     IRVLNTVEEHQDQATTSLLNLPITEEEEINEN"
     gene            complement(19094..19726)
                     /locus_tag="VBC28_000586"
     CDS             complement(19094..19726)
                     /locus_tag="VBC28_000586"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HEO6814588.1"
                     /translation="MTKIALFAGGDLTYFEYDFDYFVGIDRGSLFLLKNGLSLDMAVG
                     DFDSITEDELLYIKHYCSNIVSASAEKNDTDTELALKTIFKEFPEAQVTVFGAFGGRI
                     DHMMSNIFLPSDRDLEPFMSQIRLKDEQNIVTYLPSGKNQVSRIEGMSYVSFMPESES
                     TLQISGAKYELNKSNYFKKKMYSSNEFMTSPIEVELKDGYLIIIYSKDRG"
     gene            complement(19719..20381)
                     /locus_tag="VBC28_000587"
     CDS             complement(19719..20381)
                     /locus_tag="VBC28_000587"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000106179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEO6814589.1"
                     /translation="MSTNKIAPSILAADYANFANELKRIEETTAEYVHIDIMDGQFVP
                     NISFGADVVSSMRKHSKLVFDCHLMVVDPERYIEAFAQAGADIMTIHVEATKHIHGAL
                     QKIKEAGMKAGVVINPGTPVESLIPILDLVDQILIMTVNPGFGGQAFIPEMMSKVKTV
                     AAWRKEYGHHYDIEVDGGIDNTTIKAAAEAGANVFVAGSYLFKASDLPAQVETLRVAL
                     DD"
     gene            complement(20388..21260)
                     /gene="rsgA"
                     /locus_tag="VBC28_000588"
     CDS             complement(20388..21260)
                     /gene="rsgA"
                     /locus_tag="VBC28_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEO6814590.1"
                     /translation="MQGRIVKSLAGFYYVESDGVVYQTRARGNFRKKGQIPYVGDWVE
                     FSSQDQSEGYILSIEERKNSLVRPPIVNIDQAVVIMSAKEPDFNANLLDRFLVLLEYK
                     MIQPIIYISKLDLLDDLVVIDDIREHYQNIGYVFCYSQEELLPLLANKVTVFMGQTGV
                     GKSTLLNKIAPELKLETGEISGSLGRGRHTTRAVSFYNVHKGKIADTPGFSSLDYEVD
                     NAEDLNESFPELRRLSHFCKFRSCTHTHEPKCAVKEALTQGQLWQVRYDNYLQFLSEI
                     ESRRETYKKVIKRK"
     gene            complement(21416..22267)
                     /locus_tag="VBC28_000589"
     CDS             complement(21416..22267)
                     /locus_tag="VBC28_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEO6814591.1"
                     /translation="MLVDKKWRFEDSASYFACPKCQNPLIKESNSLKCSDNHCFDLSK
                     FGYVNLLGGKKVDEHYDKKSFENRQLVLENGYYNHILEAISKVLENNSQFHSVLDIGC
                     GEGFYSRQLVNKHEKTFLAFDISKDSIQLAAKSDQSRLVKWFVSDLANLPIQDSSIDI
                     ILDIFSPANYKEFRRVLSDDGILVKVVPVAEHVQELREKASQYLKQKDYSNQKILDHF
                     RENFEIISEQKVVQSYNCSQQERQAFIDMTPLLFSVDKTTIDWASISEITVGALIVIG
                     KKRSVSK"
     gene            complement(22271..23143)
                     /gene="rsmA"
                     /locus_tag="VBC28_000590"
     CDS             complement(22271..23143)
                     /gene="rsmA"
                     /locus_tag="VBC28_000590"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018030634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEO6814592.1"
                     /translation="MRIADKTVTRAILERHGFTFKKSFGQNFLTDTNILQKIVDTAEI
                     DKGVNVIEIGPGIGALTEFLAENAAEVMAFEIDDRLIPILADTLARFDNVQVVNQDIL
                     KADLQTQIQAFKNPDLPIKVVANLPYYITTPILMHLIESKIPFAEFVVMIQKEVADRI
                     SAMPNTKAYGSLSIAVQYYMTAKVSFIVPRTVFVPAPNVDSAILKMVRRDQPVVSVQD
                     EDFFFRVSKVAFVHRRKTLWNNLTSHFGKSEDTKAKLEKALEIAKIKPSIRGEALSIP
                     DFASLADALKEVGI"
     gene            complement(23223..23714)
                     /locus_tag="VBC28_000591"
     CDS             complement(23223..23714)
                     /locus_tag="VBC28_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000456289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6814593.1"
                     /translation="MEYNVTEALTRAGVDLATLAVKGTATMVTSKIQSIKDVKDAATL
                     RATYDDLINELLAEREQAIRIAQSYKEEYEKINIDDEDIEYLSNTLEQGISLLYNFSG
                     LNEEQERTMKQLVTLLNKDTLKTMQLIGFNYKQAIGGPLTEACATAIKNKFITQNSKG
                     KRK"
     gene            complement(23733..24293)
                     /gene="rnmV"
                     /locus_tag="VBC28_000592"
     CDS             complement(23733..24293)
                     /gene="rnmV"
                     /locus_tag="VBC28_000592"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000717750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HEO6814594.1"
                     /translation="MKKIDIQEVIVVEGKDDTANLRRFYNVDTYETRGSAIDEDDLER
                     IERLHNLRGVIVFTDPDYNGERIRKIIMNAIPTVRHAFLNRDEAKPGSKTKGRSLGVE
                     HASFEDLQKALSKVTQHFDDEDHFDITQADLIRWGFITASDSRKRREYLGNQLRVGYS
                     NGKQLLKRLRLFGVTKAEVEECMEGY"
     gene            complement(24280..25062)
                     /locus_tag="VBC28_000593"
     CDS             complement(24280..25062)
                     /locus_tag="VBC28_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEO6814595.1"
                     /translation="MIKIFDTHTHLNVENFEGKIDEEINLASELGVTKMNVVGFDQDT
                     ISKSLELSSQYAQVYSTIGWHPTEAGSYDDNIESMIISHLENPKVIALGEIGLDYYWM
                     EDPKDIQIEVFKRQIELSKEYNLPFVVHTRDALEDTYEVIKESGVGPFGGIMHSFSGS
                     LEMAQKFIDLGMMISFSGVVTFKKALDVQEAARELPLDKILVETDAPYLAPVPKRGRE
                     NKTAYTRYVVEKIAELRGITVEEVAEATYQNAVRIFRLDEKN"
     gene            complement(25244..25525)
                     /locus_tag="VBC28_000594"
     CDS             complement(25244..25525)
                     /locus_tag="VBC28_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6814596.1"
                     /translation="MSLMWDVLTGKESVRTLKKQLDKELIHSVYAPRKRSKFHDIITS
                     HHKEIDEVAINTKTAIHKVANQLDEAIKGEFRSKVEEAIESNQQKYNHL"
     gene            complement(25535..25927)
                     /locus_tag="VBC28_000595"
     CDS             complement(25535..25927)
                     /locus_tag="VBC28_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6814597.1"
                     /translation="MALIDIIEKQLADTQRKISDLDDAYHHSCCQFEEKLDDLSVRKN
                     KIINMLQETYDAVEYDLRYSNDSSDMMTLNRILDSYHDDLEQAYHKEYYALSAQEEEY
                     RANYIRQRSEHELTFEELQREKKRELMK"
     gene            complement(25918..27210)
                     /locus_tag="VBC28_000596"
     CDS             complement(25918..27210)
                     /locus_tag="VBC28_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2974 domain-containing protein"
                     /protein_id="HEO6814598.1"
                     /translation="MEKVLTDLDRVNIAKQEYELGSQLDTLVKIMSQDKVLPIGKVAH
                     VQDGGKETGEQIYTITPNGTLDKPEDVKEVTVLFKGSTAPFGGDDWKTDWFKNDIPIA
                     SKLLLKKFGSQSVSHKQGTKQLEQSAHLLKEVMNKYPNAKISVYGHSLGSMNAQYAIA
                     ELDDNQIYRVQGAWIYNGPNLYSILTDKQKEQVDKIKGVVNNYVDSKDKIGIGYPKEG
                     SDNAVGIVKIVDSKAVSGGSWVANFTNQHIWGGYQYNPDGSLKLAEDKFAYKENYSVV
                     HTNVNQRMHAIKAKLSSFKSTGKEVSKNQAIFLDSQQAKVISSGLVTISKEAVEEVKK
                     VEQKAVAKAEELYQSLKVAPTGVTELSPQELEEAYAQSGVSYASIVGEAQTFFSNKTQ
                     AAESISHAYVELENQINKGVEAMLRQDSNLSGEFKQWH"
     gene            complement(27210..27602)
                     /locus_tag="VBC28_000597"
     CDS             complement(27210..27602)
                     /locus_tag="VBC28_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1310 domain-containing protein"
                     /protein_id="HEO6814599.1"
                     /translation="MKSIKLLSFIVIILLGLGFTVGGLKVTENNKQQELWEIANSKDA
                     KNAYQKWIYAEDEDAFKENAVIKSYDIDKESIKKNPMGGISVRLIINKDPNLYITCNL
                     DRDNQGHLVSQSSHQSPQLTHLLESRGH"
     gene            complement(27777..29039)
                     /gene="dltD"
                     /locus_tag="VBC28_000598"
     CDS             complement(27777..29039)
                     /gene="dltD"
                     /locus_tag="VBC28_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HEO6814600.1"
                     /translation="MLKRLGKVFGPLVCALLLLVGLYFVFPVSQPHHLGKEKNSAVAL
                     TKAGFKSRVQKVRAFSDPKANFVPFFGSSEWLRFDAMHPSVLAEAYNRSYIPYLLGQK
                     GAASLTQYYGIQQIKGQIKNKKAIYVISPQWFVRKGANKGAFQNYFSNDQTIRFLQNQ
                     TGTTYDRYAARRLLKLYPEASMSDLIEKVADGQKLSNKDKQRLKFNDWVFEKTDAIFS
                     YLPLGKTYNQAIMPHVGKLPKAFSYNHLSRIASQDAKVATRSNQFGIDDRFYQTRIKK
                     HLKKLKGSQRHFNYTKSPEFNDLQLVLNEFSKQNTDVLFVIPPVNKKWTDYTGLDQKM
                     YQKSVEKIKHQLQSQGFNHIADLSRDGGKPYFMQDTIHLGWNGWLELDKHINPFLTEE
                     NSKPNYHINNKFLKKSWAKYTGRPSDYK"
     gene            complement(29032..29271)
                     /gene="dltC"
                     /locus_tag="VBC28_000599"
     CDS             complement(29032..29271)
                     /gene="dltC"
                     /locus_tag="VBC28_000599"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005591431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HEO6814601.1"
                     /translation="MDIKSEVLAIIDDLFMEDVSSMMDEDLFDAGVLDSMGTVELIVE
                     LESHFNIDIPIAEFGRNDWNTANKIVAGVTELCNA"
     gene            complement(29286..30551)
                     /gene="dltB"
                     /locus_tag="VBC28_000600"
     CDS             complement(29286..30551)
                     /gene="dltB"
                     /locus_tag="VBC28_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HEO6814602.1"
                     /translation="MIQAFLEKLPHLDVYGNPQYFFYLILAVLPIYIGLFFKKRFALY
                     EIIFSLSFIVMMLTGSTFNQLKSLLAYVVGQSLLVFIYKAYRKRFNHTLVFYVTVCLS
                     IFPLFLVKLISAISEDGHQSLFGFLGISYLTFRAVAMIIEMRDGVLKEFTLWEFLRFL
                     LFFPTFSSGPIDRFKRFNEDYINIPDRNELLDMLGQAIHYLMLGFLYKFILAYIFGSL
                     IMPPLKELALEQGGVFNWPTLGVMYAFGFDLFFDFAGYTMFALAISNLMGIKSPINFD
                     KPFKSRDLKEFWNRWHMSLSFWFRDFVFMRLVKLLVKNKVFKNRNVTSSVAYIINMLL
                     MGFWHGLTWYYIAYGLFHGIGLVINDAWVRKKKNINKERRLAKKPLLPENKWTYALGV
                     FITFNVVMFSFLIFSGFLDLLWFPQPHNK"
     gene            complement(30548..32083)
                     /gene="dltA"
                     /locus_tag="VBC28_000601"
     CDS             complement(30548..32083)
                     /gene="dltA"
                     /locus_tag="VBC28_000601"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HEO6814603.1"
                     /translation="MIHDMIKTIEHFAETQADFPVYDILGEVHTYGQLKVDSDSLAAH
                     IDSLGLVEKSPVLVFGGQEYEMLATFVALTKSGHAYIPVDQHSALDRIQAIMTVAQPS
                     LIISIGEFPLEVDNVPILDVSQVSAIFEEKTPYEVTHSVKGDDNYYIIFTSGTTGLPK
                     GVQISHDNLLSFTNWMISDDEFSVPERPQMLAQPPYSFDLSVMYWAPTLAMGGTLFAL
                     PKTVVNDFKKLFATINELPIQVWTSTPSFADMALLSNDFNSETLPQLTHFYFDGEELT
                     VKTAQKLRQRFPKARIVNAYGPTEATVALSAVAITDEMLETCKRLPIGYTKDDSPTYV
                     IDEEGHKLPNGEQGEIIIAGPAVSKGYLNNPEKTAEAFFQFEGLPAYHTGDLGSMTDE
                     GLLLYGGRMDFQIKFNGYRIELEDVSQNLNKSQYVKSAVAVPRYNKDHKVQNLLAYIV
                     LKEGVRDDFERDLDLTKAIKEDLKDIMMDYMMPSKFIYREDLPLTPNGKIDIKGLMSE
                     VNK"
     gene            complement(32096..32218)
                     /locus_tag="VBC28_000602"
     CDS             complement(32096..32218)
                     /locus_tag="VBC28_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HEO6814604.1"
                     /translation="MKPYLSFIGRTLLYFGILLLLIYFFAYLSRGQGSFIYNEF"
     gene            complement(32229..33416)
                     /locus_tag="VBC28_000603"
     CDS             complement(32229..33416)
                     /locus_tag="VBC28_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000490535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEO6814605.1"
                     /translation="MFSDLRKKFVFLTMSILIVVVLFLFAVSNRYNQYWDEYDAYRIV
                     KLVAKNDYLGIPGDEPIALVTIDNQKMVKIQSNNTDLTNDVIEKSSLKLLEQGKKSRK
                     WKSFIYSIKEYKDKTYTIAIMDLASYEVPYARRFLILVFTIFGFCLLAAVSLYLSRFI
                     VGPVETEMTREKQFVSDASHELKTPIAAIRANVQVLEQQIPGNRYLDHVVSETKRMEF
                     LIEDLLNLSRLDEKRSKVNFKKLNLFVLCQEVLLTYESLAYEEEKCLNDTIEDDVWIV
                     GEESQIKQILIILLDNAIRHSLSKSAIQFSLKQARRKAILTISNPSAIYSKEVMDNLF
                     ERFYQAKDDHADSLSFGLGLSIAKAIVERHKGRIRAYQEKDQLRLEVQLPIDGFWTNT
                     MIN"
     gene            complement(33416..34090)
                     /locus_tag="VBC28_000604"
     CDS             complement(33416..34090)
                     /locus_tag="VBC28_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001238605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO6814606.1"
                     /translation="MRLLVVEDEKSIAEAIQALLADKGYSVDLAFDGDDGLEYILTGL
                     YDLVLLDIMLPKRSGLSVLKRVREAGLETPIIFLTAKSQTYDKVNGLDLGADDYITKP
                     FEADELLARIRLRTRQSSLIRANQLRLGNIRLNTDSHELESKESSVKLSNKEFLLMEV
                     FMRNAKQIIPKNQLISKVWGPSDNSEYNQLEVFISFLRKKLRFLKADIEIITTKGFGY
                     SLEERT"
     gene            complement(34446..34580)
                     /gene="rpmH"
                     /locus_tag="VBC28_000605"
     CDS             complement(34446..34580)
                     /gene="rpmH"
                     /locus_tag="VBC28_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEO6814607.1"
                     /translation="MKRTYQPSKIRRQRKHGFRHRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            complement(34764..36119)
                     /locus_tag="VBC28_000606"
     CDS             complement(34764..36119)
                     /locus_tag="VBC28_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiH family protein"
                     /protein_id="HEO6814608.1"
                     /translation="MKKESISIKDILWFIIPSLFGVLLLMTPFKYNGMTTVAVSVISK
                     TINQWINAVFPIHYIILLIIFISCVLALCYRLFRPSFIEKNDLLKEISDITIFWLIIR
                     LIGLALGLMTVLHIGPEMVWGKETGGLILFDLIGGLFTIFLAAGFILPFLTEFGLLEF
                     VGVFLTPIMRPFFQLPGRSAVNCVASFVGDGTIGIALTDKQYVEGYYTSREAATISTT
                     FSAVSITFCLVVLQNVGLTDYFGHFYLTVIISSVVCAFIIPYIPPLSRQSNSYYTKPK
                     KETSEVIPDGYNKIEWGLKLAIDKSKANGNMKTFVNNASKTVLSLWFGVMPTIMTVGT
                     IALIISVSTPIFKILGTPFLPFLELLGIPEADIASQTMIVGFSDMVVPSIMAAEIHSE
                     MTRFIVATVSIVQLIYMSETGAVILGSKIPINILELFIIFIERTIISLPIIVLMAHLF
                     F"
     gene            36351..>36560
                     /locus_tag="VBC28_000607"
     CDS             36351..>36560
                     /locus_tag="VBC28_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000160532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO6814609.1"
                     /translation="MTKHKHLTLLDRNDIQSGLDRGETFKAIGLNLLKHPTTIAKEVK
                     RNKQLRESTKDCLDCPLLRKAPYVCN"
BASE COUNT        11394 a         7362 c         5839 g        11965 t
ORIGIN      
        1 cttttagtta atcccaaaaa caagactatt attttgtaat agtcactact gaataatatt
       61 tcttttatta aaaactcaca agacatttct taaagcagtt acatcttcgc gactagttga
      121 ccaactagtt gctagacgta ttacagtatg atggtcatca tatttttccc aaaaactata
      181 agctacattc tttgctaaat ctgctagttt tgtattttct acaataataa attgttgatt
      241 agttggtgat tttaaataga atgagtaacc tttttcttct aaaatgattt ttaattcctc
      301 tgctaagtcg atcgcatgct ttccaatttt taagtaaagg ttatccgtaa agaaacgatc
      361 aaaagcaagc cccagcaatc ttcccttggc taataacgca ccatgctgct ttacaattgt
      421 agtaaactgt tttggacgat ttttctttgt gaaaacaact gcttccccag caagagcccc
      481 cattttggtt ccaccaatat aaaaaacatc tgataaggct gcaatcgttg ggaaatcaac
      541 atctgtatct ttcgcagcca aaccataccc cagacgagca ccgtcaataa ataaggggat
      601 ttgataatgt ttacaaatct tcgataactc ttctagttct gctttactat aaagtgttcc
      661 atattctgtt ggatgtgata tataaaccat tcctggaaaa accatgtgtt ggtaattacc
      721 atcagcataa aaagtctcaa tataagttgc gacttcgctt gcgagtaatt tcccattatg
      781 agaaggtaaa gtcaatacct tatgccctga aaattctatg gctcctgctt catgactact
      841 cacatgacct gtttctgcag caataactcc ttcataagaa gcaagcatac tactgataac
      901 tacttgattt gtttgagtac cacccactaa aaactcaact tcagcttgtg gacaggaaca
      961 gacttctcga attttatctg ccgcttgttg acagtaacta tctgtcccat atcccgatag
     1021 accttgatca ttagtttcaa taagggcatt taaaagctct ggatgagctc ctttattata
     1081 atcattttca aaatgtaaca ttccttaact cctttattag ttaaaaaaat agcgacaata
     1141 tcatttgcta atatttttga atattcaatc attataacaa ataaaaaata tcaaaacaag
     1201 cgttttgata ttatgaacac ctataaactc tcaaaagcta aactaggttt ggcattcaat
     1261 tttaaagaag caaaattctc tttttcaaac tctatcgctg ctgctaatgc aatcattcct
     1321 gcattatccc cacataatct aagaggagga ataaccacat caatatcagg agaaatatca
     1381 gtagccaagc gttcccgaag tccttgatta gctgcaactc caccagcaac gactaaagtt
     1441 ttcactggat actttagcaa agctttttga gttttggcca ataaaatatc caaaacagcc
     1501 gcctgaaagg aagcacataa atcttcaaga accaatgctt cacccttttg ttctgcatta
     1561 tgatgtaaat tgataaatgc agattttaat ccagaaaaag aaaattcaag atgatcttct
     1621 ttgatcatcg ctctaggaaa atggtaagta tcttgaccct tatgagctaa ctgatcaatc
     1681 tcgcgacctg ctggataagt taagcccata acacggccta ctttatcata agcttctcca
     1741 acagcatcat cccgagtttc tcctactatt ttgtaatctc ccggttcaga aacatatact
     1801 aattctgtat gtcccccact gacaagcaaa gctaacaatg ggtattgaag ttccttaacg
     1861 tcacgtgctg ccattaaatg acctgccatg tggtttatag gaattagagg taatttattt
     1921 gcccaagcga aagcttttgc tgcagccata cctaccaata aggctcctac taatcccgga
     1981 ccatatgtta cagcaacagc atccaaatcg ctagcaacaa tacctgcttc ttgaagagca
     2041 tcctcaaaac aggttgttac tacttcaacg tgatgacggc ttgccacttc aggaacaaca
     2101 ccaccaaaac gtttgtgact ttcaacttga cttgcaataa tattagctag taactcttta
     2161 tcatttttta aaatagcaac actagtttca tcacatgaac tttccactgc taaaatatat
     2221 ctatctttca taatgatatt aatccatcaa tatcgtcttt tccattagta ttgaggacta
     2281 ttcatatcct ttctatcatc aactatttat cccttgcgca tcactatagc atcttcaatt
     2341 ggatcatggt aataattttt gcgctttctt aaaactacaa aaccacacct ctcataaagt
     2401 ttttgtgcta cgatatttga ataacgaacc tcaagaaaaa ctggcacctc tatatcagcg
     2461 atcattgaca ttaactggta agcataacct ttaccttgat aatttttttt aacagcaata
     2521 ttagtaattt ctacttcacc taccaattgt tgaagagcca aaaaaccaat gacttcaccg
     2581 tcattataaa caaaaaaata atctgttgaa tcttttttta aatcttctga aatctgcgat
     2641 aaactccacg gagatttgtc atagacatca gatagtacgc tccatatagc cagtgcttgt
     2701 tctgatgatt ccatctcaaa ctctcttaat atattcttct gtattcgttt cacagtggtt
     2761 ttttaaccaa ttttcctcag cttcaacacg ttttaagtat cgtggaacaa acgcatctac
     2821 attaacgctt ttcatttttt gtcctttgcg cccaattgct actgcacaag gtaaagtttc
     2881 tgtaatttta gcatgtggta aggctttctt aatctgatca aaaaatgctg caacctctcc
     2941 gacaaaatga acattggctt tattccccac ctcttgtaag acttcttcaa gagaagtgtg
     3001 acagtctggt ttaacagtat caccattttg atagaaacca acataaacat tattacgtcg
     3061 tgcatctata agtggtacca ataaatcatt ttctgaaaat ccatttgtta aagcgtacag
     3121 gctagatact ccaactaagt caatcttaag cgtataagct agcatttttg ctgtagcaac
     3181 agctacacgt aagcccgtat aagatcctgg accctctgct actacgatac ggtccaaatc
     3241 ttgaggttct aaatcaattg attgcattaa aaaatcaatg gctggcatca aattaatgct
     3301 atgattcttt ttgatattga tagtgacagt cgctaaacat tccatattgt ttagtacagc
     3361 cactgatagt gctttgcttg aagtatcaaa ggctaaaact ttcatcattt aacttccctt
     3421 acttgattct acctcttatt ttatgatata atcatgataa ttgcaacgaa tcaaggttaa
     3481 ttctattact ctataaaaac ttaataatgg tttgtcattg tctcacttaa cggcattgac
     3541 ccattttttt atagttagaa aaaccgagag ataagaaagg attattatga tttataaagt
     3601 tttctatcaa gaaacaaaag aacgcaaccc acgtcgtgag caaaccaaaa cactttacgt
     3661 tactattgat gcagctaatg aattagaagg tcgcattgct gcacgtaaac tagttgaaga
     3721 aaatacagct tacaatatcg aattcatcga gttactttca gacaaacacc ttgaatatga
     3781 aaaagaaact ggtgttttcg agctcactga gttctagaaa ggagttaaac ataatagcta
     3841 gagtaatcta cccattatga taaacttatt atgtcaaata tcaacttaaa acccgaagaa
     3901 gttggggttt atgcgattgg tggtctcggt gaaatcggaa aaaatactta cggtatcgaa
     3961 tatcaagacg aaattattat tgtagacgct ggtattaaat tccctgaaga tgatcttttg
     4021 ggaatagatt atgttatccc agactattca tatatcgttg aaaacattga ccgtatcaag
     4081 gcccttgtta taactcatgg gcacgaggac cacatcggtg gtattccttt tctattaaaa
     4141 caagctaatt taccgattta tgcaggtcct ctcgctcttg ctctaatcaa aggtaagtta
     4201 gaagaacatg gtttacttcg tgacgctaca ttatacgaaa tccacgctaa tacagagctg
     4261 acttttaaaa acttaagcgt tacatttttc cgcactactc actccattcc tgaaccatta
     4321 ggtattgtta ttcatacccc tcaaggtaag gttatttgta caggtgactt caaatttgac
     4381 tttacacctg ttggggagcc cgctgactta catcgtatgg ctgctctagg tgaagatggt
     4441 gtcctctgcc ttttatcaga ctcgacaaat gctgaagttc caaccttcac taattccgaa
     4501 aaaattgttg ggcaatctat tatgaagatt atcgaaggta tcgagggacg tattattttc
     4561 gcctcttttg cttcaaacat cttccgactc caacaggctg ctgaagctgc tgttaaaact
     4621 ggtcgtaaaa ttgctgtttt tggacggtca atggaaaagg caattgttaa cggtattgaa
     4681 cttggctata ttaaagttcc aaaaggtacc ttcattgagc caagcgaact taaaaatctt
     4741 catgctagtg aagttttaat tatgtgtaca ggtagtcaag gtgagtctat ggcagcactt
     4801 gctcgtatcg ctaatggtac tcatcgtcaa gttactttgc agcctggtga tactgtgatt
     4861 ttctcatcaa gcccaatccc tggtaataca acaagtgtca ataaattaat caataccatt
     4921 caagaagctg gtgtcgatgt tattcatggt aaaattaaca atatccatac ctccggccat
     4981 ggtggtcaac aagaacaaaa attaatgtta cgcttgatta aaccaaagta tttcatgccc
     5041 gtacacggtg agtatcgcat gcaaaaagtc catgctggtt tagcagttga cacaggtatt
     5101 cctaaagaga atatcttcat tatggaaaac ggtgatgttt tagctttaac gagcgactct
     5161 gctcgtattg ctggacattt caatgctcaa gatatctacg ttgatgggaa tggtattggc
     5221 gatattggtg cggcagttct acgtgaccgc cacgacttat ctgaagatgg tgttgtcctt
     5281 gccgttgcta ccgttgattt cgattcaaaa atgattttag ctggaccaga catccttagc
     5341 cgaggcttta tctatatgag agaatctggg gaccttattc gcgaaagcca acatatctta
     5401 ttcaatgcta ttcgtattgc actgaaaaat aaagatgcaa gtatccaatc agtaaatgga
     5461 gctattgtta atgccttacg cccattctta tatgaaaaaa ctgagcgtga gcctatcatt
     5521 attcctatgg tccttacccc tgataaataa acaaaagaac ggaaaatact ttctccgttc
     5581 tttttaattg cctatattag caaaataagt taatatatca aattacttct gggataacta
     5641 tagaatataa aagaactcca aacagtatcg tacttttata ccgtctatgt gaggagttct
     5701 ttatagatta attcggataa atgtagctaa ctacccctgg tgttactgta ttttgtggat
     5761 caaaccaacc acgataatta tcaatccatt gatgacctgc ataattagat tctaaaacct
     5821 gaattttatt agtgacagga tctacagctg taacataagc cacatgtcca taactaccat
     5881 cagtccaaca tacgatagaa cccacgcgcg gttgtgtccc tactgtataa ccagctgatg
     5941 cggcgctcgc agcccaatca ccaccgtttc cccaccagtt acctgcccat gttgccatct
     6001 cctttacacc ccatgtgcat tgacctagtg gaaacgtatt actagctgga tgtgcttgag
     6061 caatttcctc cgcacttgct aggtagtcta catctactgc atagttatat gtattcgcat
     6121 ctgcatttgt tgaagcaaat gaaaaagcgg acaccaaagc cactgttcca agcattgttt
     6181 tagactttaa ttgttttatg ttcatgtttt cctccatctg atgactccta gtttatcaaa
     6241 tccatataac agacaaatga aaacctgtaa caagttttag gcaatttttt acaataattt
     6301 acaaaaaaac tagttttaga ctttacaaaa taaagcctaa aactagttat aaacattctt
     6361 atgattaata taaatctagg taattatcaa tttcccattg tgaaacataa gtcgcataac
     6421 ttgcccattc aatacgctta gcgtctaaga aattagtata gatatgagtt cctagggccg
     6481 cttttaccac ttcatcttct tcaagtgctt taagagcatt atgaagagta gatggtaaat
     6541 caacaatacc tgcttgacga cgttcttcta cagtcatagc atagatattt gtctcaattg
     6601 gttctggtgc ttcaatttta ttctcgattc cctcaagtcc tgatcccaaa agtactgata
     6661 aagcaagata aggatttgct gttgggtcaa ccgagcgcaa ttctaggcgt gttcccatac
     6721 ctcgagatgc aggtacacga ataagtggcg aacgattgcg acctgcccaa gcaacgtaaa
     6781 ctggtgcttc gtatcctggt accaaacgtt tataagaatt aacagttgga ttaatgatag
     6841 ctgtgtaatt gtaagcgtgt ttcattaacc cacctaaaaa gtagtaggca tcctctgata
     6901 attgcattcc acgtggatct tctggatcaa agaaagcatt gttcccttca ttatcaaaaa
     6961 gagacatatt acaatgcata cctgaaccat tgataccaaa cttaggtttt gccataaaag
     7021 tagcatataa accatgctta cgagcaattg ttttaacaac caatttaaat aattgaatgt
     7081 tgtcacaggc ttttaaaaca tcatcatatt taaagtcaat ttcatgctgt ccaactgcta
     7141 cttcatgatg actagcttca acttcaaagc ccatttgtgt taagacatta acaatttcac
     7201 gacgtgtatt atctgctaag tctgttggag ctaagtcaaa gtagcctcct ttgtcattaa
     7261 catcaagtgt tgggttacca ttttcatcca ttttaaatag gaaaaattca ggttctggtc
     7321 ctaagttaaa tgacttatag ccaatctctt gcatacgttt catattacgc ttaagatttc
     7381 cacgtggatc tccggcaaaa ggttctcctt ctgctgtata gatatcacag attagacctg
     7441 caacagctcc attttcatct ccccaaggaa agacaatcca agtatctaaa tcaggatata
     7501 aatacatatc agattcattg atacgaacaa agccctcaat agaagatcca tcaaacattg
     7561 ctttatttga taaaactttg tctaattgtt catctgtagc cggaatttcg acatttttca
     7621 ttactcctaa gatatctgtg aacatcaaac ggaggaaggt aacatttttt tccttaactt
     7681 cgcgacgaat atcttctgct gtgatagtca tatagtctcc ttaatatcta gcttgactgc
     7741 atgtttacaa atgcatattt ccaatgtgct gtgaaggact tgaaaaacgc ccttgattac
     7801 ggagctcatc ttgtaaaagt cgacgaacct cggcatctga taatgacttt tgtttctctt
     7861 tggacttatg ttgtctttgc atgtattcat ttttaatatc agaaatatgc aaaccatcgc
     7921 taatgaagtc ttttatttcc agtaagcggt ccatatcatt cagtgaaaac atgcggcggt
     7981 taccttcagt acgttcaggt gtgattaacc cttgatcctc ataatagcga atttgtctgg
     8041 cagtcaaatc tgtcaacttc ataacagctc caatcgggaa tacagccatt gtacgcctaa
     8101 gttctctttc tttcatgatg gcctcctttc attaaaccat tataaacttt aaaaagtttg
     8161 atgtcaactc tcatgtgaga ttttatgaca tcaaactata atttgttatt ttttagtttt
     8221 ttccgaagta tttcaatatc tgtattaacc atcattgcaa taaaaacacc acaaaaagtt
     8281 tcaaaaatac gtgcaaaaac atatagaatt gtctctccgc ttgggataga taaagttata
     8341 ataagtaatg cggctgttcc cccaataata cctgatttat tattacaagc aacattaatc
     8401 atgatacata acatggtaca aataggaaca attaataaag tgacccaaaa agcttgatga
     8461 aatatctcat tgaatgcaaa aaatactaag gagagtaagc cacctatact attaccaata
     8521 attctagaaa aaccaaagtg cacgctttta tcaaagtctt ctcttaaact aaatacagca
     8581 gttagagcac caatttgaag ccctttccaa ccaaataaat gaaaaaccaa taaaactaaa
     8641 aagaccgata agcctgtttt aaatgttctc attcccaatt taaacttttt aatatcaaac
     8701 ttgtaattag ataataattt ttttaacgtt ttcataccta tcccttcact atatattcta
     8761 acatatcaat ccataaaacc ctttttgtaa acgttttatt tattactagt atatctttta
     8821 taaaccaatt tatatcaaat cgtcataaac ttttgaaaat tgcagtgtca accaaatacc
     8881 actatatcta aaaactagta aatgataaat gaagtcaagg aaagttcaat aactctacat
     8941 tatcaactaa aaaaagacat gcatcagcat gtcctttaat ataaattata atgactatta
     9001 tttttcagtc aatgctgcca aacctggtaa tactttacct tcgagcaatt ccatgcttgc
     9061 tccaccacca gtagagatcc atgagaattt gtcagcacgg ccaaggttga tagcagctgc
     9121 tgctgaatca ccaccaccga tgattgattt aacgcctggt tgtttaacga tagcgtccat
     9181 tacaccgatt gtaccagctt ggaagtcagg gttttcaaag acacccatag gtccgttcca
     9241 tacaactgtt ttagcaccag taagtgcttc atcaaattta gcgattgatt taggaccgat
     9301 gtcaagacca aggaaccctt ctgaaactgc ttcaccttca gtgtcgcgaa cttcagtata
     9361 accagcaaat gcgtttgctt cttttgagtc aactggcaag atcaatttac cgtttgattt
     9421 ttcaaggagg tctttagcaa catccaattt gtcttcttct acaagtgagt taccgatttc
     9481 gataccttga gctttgtaga atgtgtaagt cataccacca ccgataagaa ctttatcagc
     9541 tttttcaaga aggttttcga taacaccaat cttatcagaa acttttgagc caccaagaat
     9601 agctacgaat gggcgttctg gagtttcaac tgcttcttgg atgtaagcaa tttcgttttc
     9661 aagaaggaaa ccagctacag ctttttcaac gtttgctgaa atacctacgt ttgatgcatg
     9721 agcacggtgt gctgtaccaa atgcatcgtt aacgaagatt ccatctccaa gtgaagccca
     9781 gtatttacca agttcttcgt cattcttaga ttctttctta ccgtcaacat cttcaaaacg
     9841 agtgttttca accaaaagaa cttgtccatc ttccaaagca ttgattgctt cttctaattt
     9901 tgcaccacga gtaacacctg ggaatacaac atcttgacca agtttagcag ctaaatcagc
     9961 agctaccggt gcaagtgatt ttccttcttt gtcagcttct tctttaacac gtccaaggtg
    10021 agagaagagg atagcacgac caccttgttc gatgatatac ttgattgttg gaagagccgc
    10081 agtgatacgg ttgtcgttag tgataacgcc gtctttcaaa ggcacattaa agtcaacacg
    10141 aacgaggact tttttacctt tcaaatcaac gtctttaaca gtcaatttag ccattagtaa
    10201 aactccttat ataatttgat attatttatt ataacatatt tttgtgaaca gttgtaatta
    10261 ttgattactt tttcacaaat agatttcata gattttccta gttgttttca agagaattcg
    10321 tatacttatt aatcatacga atgcaacatt ttttgtcgtt ctttcaattg tttttgaaca
    10381 tccagatgtt ttccttgata aatagcactt tcccaagaac cgtacatagg atttgggaaa
    10441 acaataaatt tactaccaaa ctcactttga agtttagtta gtagttgttc tctatctgta
    10501 ctagatgatt tagaaaaatc agcaaaatca actaaattat ctccaaaaag cataattaaa
    10561 ttggtatctt tttgaactgc ctgacggcga ctctctttag atttcattcc ttttttaagg
    10621 aaaagcaagt ggtctttccc ttgaacaggt ataccttcct tctctaaatt ttctttagtc
    10681 gcatcaactt gagcatctgt acgatctgag acataataaa tttttattcc cttttcatta
    10741 gcatacttca aaaattcttt ggcacccgca acagccttag ctgatttctt ttgcacccat
    10801 ttatcccaac tctctggcgt gaaactagag ccatctttaa tattctttgc ttgatatggg
    10861 ctattatcta aaactgtttc atctaaatct aagataattg aatatggttt ttcactgggc
    10921 ttttgtaacc aatcatctaa cttcatttta gcaacattat aaccttgtaa atataaagcc
    10981 ttggcttctg cagaattttg ataccaaagg acagacatag tgttttcctt agccaacagt
    11041 ttattagaac ttagagaaat atttttaaat ttcgtctttt gctccacctt atctggagac
    11101 atatcagctt ttttagtact gtggtttgac tgacatgctg tcatcataaa tacagcaaca
    11161 gataacgata aaactgaaaa aacttttgta gtcttcataa aacttctcct ctatattttt
    11221 tcaattaaat cataccactg cttactttca aaatcaaacc ttattttaac ttatagtcaa
    11281 tataattcat ttttcattta ttttctgata attttctata ataaaatgaa aaaacattcg
    11341 tattattttt tatttataaa aataaaagag ggataaatcc ctcttttaca tcatattaat
    11401 taaaaattat tttgcaattt ttgcaaagta ttcaagtgta cgaacaagtt gtgaagtgta
    11461 tgacatttcg ttatcgtacc atgaaacaac tttaaccaat tggttaccgt caacagtttg
    11521 aactttagtt tgagtagcat caaacaatga accgtatgaa ataccaacga tatcagatga
    11581 tacgattgga tcttcagtat aaccgtatga atcgttagct gctgctttca tagctgcatt
    11641 tacttcttcg acagttacgt ctttttcaag agttgcaacc aattcagtta ctgatccagt
    11701 tggaacagga acacgttgtg cagcaccatc aagtttaccg ttcaattctg ggataacaag
    11761 tccgatagct tttgcagcac cagttgagtt aggaacgatg tttgcagcac ctgcacgagc
    11821 acgacgaagg tcaccaccac ggtgtggtcc gtcaaggatc atttggtcac cagtgtatgc
    11881 gtggatagta gtcatcaaac cttgtttaac accaaagttg tcttgtaaag ctttagccat
    11941 tggagcaaga cagtttgtag tacatgaagc acctgagata actgtttcag ttccatcaag
    12001 gatatcgtgg ttagtgttga aaacaactgt tttaacgtcg tttccaccag gagctgtgat
    12061 aacaactttt ttagcaccat tttcatggat gtgttgttca gctttttctt ttgatgcaaa
    12121 gaaaccagtt gcttcaagaa cgatttctac gccatcagta gcccagtcaa tgtttgctgg
    12181 ttcgcgttca gcagaaactt taacaaattg accgttaact tcgaatccac cttctttaac
    12241 ttcaacagta ccgtcgaaac gaccttgagt tgtgtcatat ttcaacaagt gtgcaagcat
    12301 gtttggatct gtaaggtcgt tgatacgagt aacttcaaca ccttctacgt tttggatgcg
    12361 acggaatgca agacgaccga tacgaccgaa accgttaata ccaactttaa ctaccattag
    12421 tgatttcctc cttatgaaaa tctcaaatat tttattttag ggatccccct aaccaatgtg
    12481 aaaagattaa cttgtacata tgtgcaaaaa tctttcaaca caattattat atcttttttc
    12541 gagaaaaatt gcaatgaatt taccactttc aaaaagaaaa aaagatactc tttcgagtac
    12601 ctttcataag ttttcagaaa ttattcacca gagtttttct tgatgatttc ttcttgaact
    12661 gacttaggaa catcttcgta gtggtcaaat accatcatga atgtgccacg tccttgtgtt
    12721 gcagaacgaa gaactgttgc ataaccaaac atttcagcaa gtggaacaaa ggcacgaaca
    12781 acttgtgtat taccacgtgc ttccatacca tcaacacgac cgcgacgtgc agtcacgtga
    12841 cccataacgt caccaagatt gtcttctgga gctgtaattg taacaagcat cattggctca
    12901 aggatagctg gttgcgctga tttagcagct tctttaagtg caagtgatgc agcaatcttg
    12961 aaggctgtct cagatgagtc aacatcatgg tatgaaccat cgtaaagttt agcttttacg
    13021 tcaaccattg gatatccagc aagaacaccg tttgccatag attctacaag tcctttttca
    13081 acggctggaa taaattcacg aggaaccaca ccaccgacga tagcattttc gaattcgaat
    13141 cctttacctt cttcgtttgg tgtaaattca atccaaacat caccaaattg acctttacca
    13201 ccagattggc gtttgaagaa tccacgtgct tgtgtagaag cgcggaatgt ttcgcggtat
    13261 gatacttgag gtgcaccaac gtttgcttca actttgaatt cacgtttcat acggtcaaca
    13321 aggacatcaa ggtgaagttc acccattcct gagataactg tttcaccagt ttcaacgtta
    13381 gtttcaacgc ggaacgttgg atcttcttca gcaagttttt gaagggcgat acccatttta
    13441 tcttggtcag ctttagattt tggctcaacc atcaattgga taactggttc tggaacttca
    13501 attgattcaa gaataacttt tgatttttca tcagtcaatg aatcacctgt agttgtatct
    13561 ttcaaaccaa cagccgctgc gatatcccca gcataaacag tttcaatttc ttgacgacta
    13621 tttgcgtgca tttgtaagat acgtccgata cgttcacgtt tacctttaga tgtattcaac
    13681 acgtatgaac ctgaattaag aacacctgag tacacacgga agaatgttaa gcgacccacg
    13741 aaaggatctg tcatgatctt gaaggcaagc gctgcaaatg gctcttcatc tgaagcagga
    13801 cgtgtttctt cttcgtcagt atcagggtta atacctttga ttgcaggaat atcaagtggg
    13861 cttggaaggt aatcaataac agcatcaagc atcaattgaa cacctttatt cttgaaggct
    13921 gaaccacaca atacagggta gaattcaaca ttgatagttg ctttacgaat agcagccatc
    13981 aactcttcgt tagtgatttc ttcaccttca aggtatttca tcatcaaatc ttcatcagta
    14041 tcagcaactg cttctactaa tttttcacga tattcatttg cttgatcaac atactctgct
    14101 ggaatatctt cttcaaggat atctgtacca aggtcattag tatagatttc agctttcatt
    14161 ttgattaagt caataatacc acggaaatcg tcttctgaac caattggcaa ttgaattggg
    14221 tgtgcattcg cttgaagacg gtcatgaagt gattgtactg aatagaggaa gtcagcaccg
    14281 attttatcca ttttattagc aaaaacgata cgtggaactc cgtattctgt tgcttgacgc
    14341 caaactgttt cagtttgagg ttctacacct gattgagcat caagaactgt taccgcacca
    14401 tcaagtacgc gaagtgaacg ttgaacttca attgtgaagt ccacgtgtcc tggtgtatcg
    14461 atgatgttaa cacggtgacc atcccattgt gctgttgttg cagctgatgt aattgtgata
    14521 ccacgttctt gttcttgttc catccagtcc atttgtgaag caccttcgtg tgtttcaccg
    14581 attttgtgga ttttaccagt atagtaaaga atacgctcag ttgttgttgt tttaccagca
    14641 tcgacgtgag ccatgatacc gatattacga gttttttcaa gtgaaaattc gcgagccatt
    14701 tggtttctcc tatattttaa attatttact tctttattat aacactattt ataaaaaacg
    14761 gataggcagg acctacccgt tctaattgct aacattgcta tctaccaaac aatttgttta
    14821 gtagctatga taagtctcaa aaagagagta atcttaccaa cggaagtgtg caaaggcacg
    14881 gttagcttca gccattttgt gagtatcttc acgtttctta actgatgctc ctgtgttgtt
    14941 tgcagcatcc atgatttctt ttgcaagacg atctttcata gtgtgttcac cacgagcacg
    15001 tgatgcgtta accaaccaac gaagtcctaa agttgtacga cgttctggac gaacttcgac
    15061 aggaacttgg tagtttgatc caccaacacg gcgtgcacgt acctcaagga caggcatgat
    15121 gttttccata gctgtttcga acacttcaag agcatctgtt ccagtagctt ctttaattgc
    15181 ttcaaaagca tcatatacga tagttgcagc agtaccacgt ttaccatcaa gcattacacg
    15241 gttgataaga cgtgtaacga tttttgagtt atacaatgga tctggcaata cttcgcgttt
    15301 aggcgcttga tttttacgac tcattttctc ttatccccct tctaattacc ctttaggacg
    15361 tttagcaccg tatttagaac ggccttgttt acgatcagct acacctgcag tatcaagtgc
    15421 accacgaacg atatggtaac gtacccctgg aaggtctttt acacgtccac cacggataag
    15481 cacaacactg tgttcttgca agttgtgtcc gatacctgga atgtaagcag taacttcaat
    15541 aaggttgctc aaacgtacac gagcaaattt acgaagggca gagttaggtt ttttaggtgt
    15601 catagttcca acacgagttg caacaccacg tttttgtggt gcagataatt tagtgtgaac
    15661 ttttctgtga ctgttgtaac caatattcaa agcaggtgaa tctgattttt caactttaga
    15721 tttacgtggt ttacgtacca actggttaat tgtaggcatc tacatttctc ctgtaagttt
    15781 tttatttttg gttgatatag cacttggtga cagcctatat ctgcgtgtac ttttgcaaca
    15841 attgtcagca cgtctctgta cacttttgag agaccaaaag taaaagtacc gtttatcata
    15901 gtatcatttt acacataatg tgtcaagctt ttattacttt tacaagcaaa aagaatcaag
    15961 gatgtcacct tgactcttta atcaaatata ttccccgctt ctacagagac tctattattt
    16021 tttacatcaa tttcagatac tctcagaaga gatttatagg ccatcttctc acgttgttcc
    16081 tgagcatttt ctgcaaatac agcgacacct actaatgtgc tatcgaattc tgagagtaaa
    16141 ctaatcatcc ccgaaacagt acctccacct ttcaagaaat catcaactat taaaactcga
    16201 ctgttaggtt ttaagctacg cttagaaaga aacatttttt caatacggcc acttgaacca
    16261 ctagcgtagt ttactgacac agtagaacct tccgtaatct tcaaatcacg tctcacaata
    16321 acaaaaggaa catctaaaac atttgctact gcgtttgcta aaggaacacc ttttgttgca
    16381 accgtcatga cagcatcaat tttttgacct ctgtaagcgt tcgctataat gcgaccaata
    16441 ctttgtaaca tttttggtgt actgagtaga tctgaaagat aaatgtaacc tcccggaaga
    16501 atacgatcgt tttcagataa acgctgtctt aattcttcaa cgatttcttt cgcttctgct
    16561 tcagcaatag ttggtgtaaa gataacacct ccacttgcac cagtcacagt tttaatatct
    16621 ccgatttgag cttgttcaaa agcttttttg ataatagcaa tatcttctga aatcgaagat
    16681 ttcgcggcac cgtatttact agcaaatgta tttaaactag ttaatgtata gggattattt
    16741 attaggtaat ttgaaataac aaccatgcgt tcgctacgtc ttaatttcat attttctcct
    16801 tattcatagc attctttaca tatagtataa cataacaaat gctaaaaacg aacattttat
    16861 tttaggtctt aattattcta cctaaattat ttaatgttcg gtttataaaa agaacggtta
    16921 tccatggcaa aaatgcgatt tgtcatttca ccttcgttga cacggtttaa agctgttgtc
    16981 atcatcatca tatttgcatc aatgttatcg atcatatgga taatttctgc ctccatgatt
    17041 ctaggacgaa ccgggcttcc atattctaat tgtccatgat gactcagaat aacatggcgt
    17101 aacactgtaa cctcttcttt tgtatcatca atattaagct ctgctagtat ttttgttaat
    17161 tcctcatcaa taagtgaaat atgaccgata agattacctc gaatagtata ttctgtatta
    17221 tcaggacccg ataactcaat gaccttggct aaatcatgta gcataatacc agcaaacatc
    17281 aaacttttat taagttctgg atagatatct ccgatactat ctgccaaacg aaccattgtt
    17341 gccgtgtgat atgccaatcc tgattcaaaa gcgtggtggt ttgttttggc ggctggatag
    17401 gtaaaaaatt ccttattgta tttacgataa agtgctctta ccacacgctg ccaggttgca
    17461 ttttcaattt taaagagcat ttgttccatg tactctctca cgttgtcaac attgattgga
    17521 ggtttttctt taaaatctct aggatcatta ggttcaccat ctttgatgtt tcttaatgta
    17581 atttggttga cttggggagt tccattataa acttcacgac gccctttcat atgaacaatt
    17641 ttaccggcta caaactcttc tacattatag gtctgtgcat cccacatatt tcctgaaatt
    17701 tcgccagtat catcttgaaa ggtaaaggca ataaagtctt tacctgcacg cgttttacga
    17761 acttctgctt tctttataag gtaaaacccc tcaaaaagct cgtctttttt catttggtta
    17821 attttcatta atttcctctt cttctgttat aggaaggttt aacaagctag tggttgcttg
    17881 atcttgatgt tcttcaactg tatttaagac tctgataata gcgtttgtcc gcgttgtgag
    17941 taaactatca atacttttac ttgctgtatt taattgtttt tgagcttttg ataacatgcc
    18001 accaaatttt ccaaattcaa ccttgacatt ccctaaaatt ttactaatat cattagcatt
    18061 cttttgaata tttaaggttt taaagccaac agataatgaa tttagtaaag cagataaggt
    18121 tgacggtcca gctactacaa tattttcgtc acgtcttaga ctatcaaaga atgttgcatt
    18181 tcttaccact tcagaataga gcccttcagt tggtaagaac atgataccaa aatttgtcgt
    18241 ttcaggagga tttaagtact tattgtttat atcttttgca aatttacgaa tacttgccag
    18301 taaagattta cgatagagtt ctatttgaac cttgtcaccc aattcataag catcttccaa
    18361 tcggtaataa tcttctagag gaaacttaga gtcaataggc aaatagatat aatctccctg
    18421 accatttcca ggtaatttaa tagcatattc aacacgctca gaagagccag acaccgtagg
    18481 aaattctctc tcatattgac taactgtcat aatatcttct ataatttggc cgagttgtaa
    18541 ctcccctaat attccccttg tcttagtatt tgacaagact ttgttcagtg taccaacatc
    18601 ttgggcaaca gttttcatct ccccaagacc ttgattgacg ctctctagtt gacgcgatac
    18661 agtttcaaaa gaagtttgca aacgcgtttg tagcgttttt tctagctttt cctcaacagt
    18721 ttggcgcatt tgatccaagc gtttttcatt ggaatcttgc attttctgaa ctgactgaga
    18781 taaattggag ttaatcaatt ccaagcgttt gtctgatcga tctcgactct ccgttaaatg
    18841 ctgatttaat tctgtacgca tatcggtaag caactgataa atttcctgtt gcatacggtt
    18901 tagctgatta gtcaacgtta gaatttgatc tttttgcgcc acttctatct gatatgtcag
    18961 ttggtctgat agattatcag cattatcttc aagttgtttt tcaacggact ttgttaattc
    19021 cgtcattttt tggtaagaaa ttatcgtcac tgtcagacta gctagtacaa tgatcaaaat
    19081 gatgacaagc atattaacct ctatccttac tataaataat gattaagtaa ccatctttga
    19141 gctctacttc aattggcgaa gtcataaatt cattactgga atacattttt ttcttaaaat
    19201 aattactttt gttcaattca tatttagccc cagaaatctg caaagtagac tcactctcag
    19261 gcatgaacga gacataggac atcccttcaa tccttgagac ttgatttttc ccggatggta
    19321 gataagtcac tatgttttgt tcatctttga gtctgatttg tgacataaat ggttctagat
    19381 ctctgtcact tggtaaaaag atattagaca tcatatggtc aatcctgcca ccaaatgctc
    19441 caaatacggt cacctgagct tctggaaatt ctttgaaaat agtttttaaa gctaattctg
    19501 tatctgtatc atttttttct gcactagcag aaacgatatt gctacaatag tgtttgatat
    19561 aaagcagttc atcttctgtt atagaatcaa aatcacccac tgccatgtcc aaagaaagcc
    19621 cattttttaa taaaaatagg cttccacggt caattccgac aaaatagtca aaatcatatt
    19681 caaaataagt taagtcaccg ccagcaaata aagcaatttt agtcatcgag cgccaccctt
    19741 aaggtttcta cttgtgcagg taaatcactt gctttaaaga gataagaacc tgcaacaaag
    19801 acatttgcac cagcctctgc tgctgcctta attgtagtat tatctatacc gccatcaact
    19861 tcaatatcat aatgatgacc gtactctttg cgccatgctg caactgtctt tacttttgac
    19921 atcatttctg gaataaaggc ttgtccacca aagccaggat taacagtcat aattaaaatc
    19981 tggtcaacca aatctaagat aggaataaga gattctacgg gtgttcctgg gttaataacc
    20041 acacctgctt tcattcctgc ctcttttatc ttttgaagcg caccatgtat atgtttcgtt
    20101 gcttcaacat gaatggtcat gatatcagca ccagcttgag caaaagcctc aatataacgt
    20161 tctggatcaa caaccatcag atgacaatca aaaacaagtt tactgtgttt acgcatgctg
    20221 gatacaacat ccgcaccaaa actaatatta gggacaaatt gaccatccat gatatcaata
    20281 tgaacgtatt ctgctgttgt ctcttcaata cgtttcaatt cgttagcaaa attagcatag
    20341 tctgctgcta atatcgatgg ggcaatttta ttggttgaca tagaaaccta ctttctttta
    20401 ataactttct tatatgtttc acgacggctc tcaatttcac ttaaaaattg gaggtagtta
    20461 tcatagcgga cttgccataa ctgaccttga gttaaggctt cttttacagc acatttaggc
    20521 tcatgtgtat gcgtacacga gcggaattta caaaaatgac taagtctacg aagctctgga
    20581 aaggattcgt ttaagtcctc tgcattatcc acttcatagt ccaaagaaga aaaaccagga
    20641 gtgtctgcaa tttttccttt atgaacgtta tagaaactaa ctgctcttgt ggtgtgtcgg
    20701 ccacggccta aactacccga aatttcacca gtttcaagtt tcaactcagg agcaattttg
    20761 ttgagaagcg ttgattttcc gacacctgtc tggcccataa aaacagtcac tttatttgct
    20821 aacagaggca agagttcctc ttgagaataa cagaaaacgt agcctatatt ctgataatgt
    20881 tcgcgaatat catcaataac aaccaaatcg tctaacaaat ccaacttgga aatataaatg
    20941 ataggctgaa tcatcttata ttctagaagt actaagaacc ggtctaataa gttagcgtta
    21001 aaatcaggtt ctttagcaga catgataacg acagcttgat caatattaac aataggtggt
    21061 cgtacgaggc tatttttacg ctcttcaatt gatagaatat atccttctga ttgatcttgc
    21121 gaagaaaatt caacccaatc cccaacgtag ggaatttgac ctttttttct aaaatttcca
    21181 cgcgcacgtg tctgataaac aacaccgtca gattccacat aataaaaacc tgctagcgac
    21241 ttgacaattc taccttgcaa ttgttctcct taattgttta gaataccact attatatcat
    21301 aaaatgagaa ataagctcta ctctcagaga taagctaaat agaaactatt tgcaggtgtt
    21361 cgatttctct caaataacct caattttatg aattcccaac ataaaagcag tgatcttatt
    21421 tagatacact gcgctttttt ccaattacta ttaaggcacc tactgtaatt tctgatatac
    21481 tcgcccaatc aatagtcgtt ttatctacgc tgaataacaa cggtgtcata tcaataaaag
    21541 cttgacgttc ttgttgagaa cagttatagc tttgaacaac tttctgctca gaaataattt
    21601 caaaattttc tctaaagtga tctagaattt tttgattaga gtaatctttc tgtttcaagt
    21661 attgactagc tttttctcgc aattcttgaa catgttcagc tacaggaaca acctttacaa
    21721 ggataccatc atcactcagc acccgcctaa attctttata atttgcagga gaaaaaatgt
    21781 ctaaaatgat atcaattgat gagtcttgaa taggaaggtt agctaaatca gaaacaaacc
    21841 atttcacaag cctagactga tcacttttag cagctaattg aatcgaatct ttagaaatat
    21901 caaaagccaa gaaagtcttc tcatgcttgt taactaattg acgagaataa aagccctctc
    21961 cacaaccaat atctaaaacc gaatgaaact gagaattatt ttccaaaacc tttgaaatag
    22021 cttctaaaat atgattataa tacccgtttt ctaagacaag ctgccgattt tcaaatgatt
    22081 ttttatcata atgttcatct accttctttc ctccaagaag attaacatat ccaaatttgg
    22141 ataaatcaaa acaatgatta tcagagcatt tgaggctatt actttctttt atcaagggat
    22201 tttgacattt aggacaagcg aaataacttg cactatcttc aaaacgccac ttcttgtcaa
    22261 ccaacatagt ttatatccca acctctttca aggcatctgc taaacttgca aaatcaggga
    22321 tagataaggc ttctccgcga atagatggct taatttttgc tatttctaat gctttttcca
    22381 attttgcttt agtatcttct gattttccaa aatgactcgt tagattattc caaagtgttt
    22441 tacgacgatg tacgaaggct acttttgaca ctctgaaaaa gaaatcctca tcttgaacac
    22501 tcactactgg ttgatctcta cggaccattt tcaaaatagc tgaatctaca tttggagcag
    22561 gaacaaaaac agttctcgga acgataaatg atactttggc agtcatataa tactgcactg
    22621 cgattgacaa agaaccgtat gctttagtat ttggcatagc tgaaatacga tctgccactt
    22681 ctttttgtat catgaccaca aactccgcaa aaggaatctt gctctcaatc aagtgcataa
    22741 ggattggtgt tgtgatgtag taaggtaggt ttgcgaccac cttgataggc agatcaggat
    22801 tcttgaaagc ctgaatctgg gtctgcaaat cagccttgag gatatcctga ttgaccactt
    22861 ggacattgtc aaaacgcgca agcgtatcag caaggatagg aatgagacga tcatcaatct
    22921 caaaagccat aacctcagct gcattctcag ccaaaaactc tgtcaaagcc ccgataccag
    22981 gaccaatctc aatcacgttg acacccttgt caatctctgc cgtgtcaaca atcttctgta
    23041 aaatattagt atcggtcaaa aagttctgac cgaatgattt cttaaatgta aacccatgac
    23101 gctccaaaat agcgcgggta acggttttat ctgcgattct cataattcct ctatttctta
    23161 atattgatca tttttgaaat aacatttgag gctatttgtg atattacaac tggattatac
    23221 cactacttcc ttttgccttt actattctga gttataaact tatttttaat tgcagttgca
    23281 caagcttcgg ttagtggtcc tcctattgct tgcttataat taaatccaat caactgcatt
    23341 gtttttaaag tatctttatt caataatgta accaattgtt tcattgttcg ttcctgttcc
    23401 tcatttagac cagaaaaatt atataacaat gagattcctt gctcgagagt attggataga
    23461 tattcaatat cctcatcatc tatattaatt ttttcatact cttctttata tgactgtgca
    23521 atgcgaattg cttgctcccg ttcagctaat aattcgttaa ttaaatcatc ataagtagct
    23581 ctcaatgttg cagcatcttt tacatccttt attgattgaa ttttactggt taccatagtt
    23641 gcagtccctt tgactgcaag cgttgctaaa tctaccccag ctctagtcaa tgcttccgtt
    23701 acattatact ccactatttt tctccttttc tattaatacc cctccataca ctcttcaact
    23761 tctgccttgg tcactccgaa gagtctgagg cgtttgagga gttgtttacc atttgagtag
    23821 ccgacacgga gttgattccc aagatactca cggcgttttc ggctatcgct agcagtaatg
    23881 aatccccaac gaatgaggtc tgcttgggtg atatcgaaat gatcttcatc atcgaaatgc
    23941 tgagtcactt ttgagagtgc cttctgcaaa tcttcaaagc tggcatgctc gacacccaga
    24001 gatcgtccct tggtctttga gcctggcttt gcctcgtctc gattgaggaa ggcatggcgg
    24061 actgtcggaa tggcattcat gatgatttta cggatacgct cgccattata atctgggtct
    24121 gtaaagacga taactccccg caaattgtgc aaacgttcga tacgctctaa atcatcttca
    24181 tcaattgctg aacctctagt ttcataggta tcaacattgt aaaagcgtcg cagattagca
    24241 gtatcatctt tcccttcgac aacgatcact tcttgaatat caattttttt catcaagtct
    24301 aaatatcctt actgcatttt ggtatgtagc ctccgctacc tcttccacag ttatgcctcg
    24361 tagctctgct attttttcaa cgacatatct cgtataagcg gttttgtttt ctcgacctct
    24421 tttaggtaca ggagccaaat aaggagcatc tgtttccact aatatcttat ctaatggcaa
    24481 ctctcgtgca gcttcttgta catctaatgc cttcttaaag gtcacaacac cagaaaagga
    24541 aatcatcatc cctaaatcaa taaatttctg agccatttcc aaagaaccag aaaaagagtg
    24601 cattatacca ccaaaaggcc caactccaga ttccttaatg acttcataag tgtcttctaa
    24661 cgcatcacgt gtatgaacga caaaaggaag gttatactct tttgaaagtt caatttgacg
    24721 cttaaacacc tcgatctgaa tatctttggg atcctccatc caataataat ccagaccaat
    24781 ttcaccaagt gcaataacct tgggattttc taaatgactg ataatcattg actcaatatt
    24841 atcatcataa gaaccagctt cagttggatg ccatcctatt gtgctatata cctgtgcata
    24901 ttgggaagat aattccaaag attttgaaat ggtatcttgg tcaaagccga caacattcat
    24961 ttttgtaaca ccgagttcgc tagctaaatt aatttcctcg tctatctttc cttcaaaatt
    25021 ttctacgtta aggtgggtat gggtatcaaa tatttttatc atatcatcgt tctttctttg
    25081 cttatcgtag ctacaagcat taatctttat tattataaca taagactgaa gcattctgat
    25141 cagaaagagt aaatcaaaaa agtcaaaaca tttttgctct gacttttgaa tacttgctgt
    25201 aaaaatcctc tacaagtgtc tagctaatgg tagagaaact agactataaa tgattatatt
    25261 tttgctgatt gctttcaata gcctcctcaa cctttgaacg aaattctcct tttattgcct
    25321 cgtccaattg attagcaact ttatgaatag ctgtctttgt gttgatggct acttcgtcta
    25381 tttctttatg atgcgacgta ataatatcgt gaaacttaga tctcttgcgt ggcgcgtaca
    25441 cgctatgtat caattcttta tccaattgtt tttttaaagt ccttactgac tccttaccag
    25501 tcaggacatc ccacattaaa ctcaataatt tctcctactt cattaattct cttttctttt
    25561 ccctttgcaa ctcttcaaag gttagttcat gttctgaacg ttgccgaata tagttagctc
    25621 tatattcttc ttcttgagcg gacaaggcat agtactcttt atggtaagct tgctctaagt
    25681 cgtcatgata agaatctaaa atacgattga gcgtcatcat atctgagcta tcattactat
    25741 atctcaaatc atattcaaca gcgtcgtaag tttcttgaag catattaata atcttatttt
    25801 ttcttactga gagatcgtct aatttttctt caaactggca acaactatgg tgataggcat
    25861 catctaaatc tgatattttt ctctgagtat cagctaattg tttttcaatg atatcaatca
    25921 gtgccattgt ttaaactccc ctgacaagtt actatcttgt cgcaacatag cttcaactcc
    25981 tttattaatt tggttttcta attcaacgta agcgtgagaa atgctttctg ccgcttgagt
    26041 tttatttgag aaaaaggttt gagcttctcc gacaatgctc gcatagctaa cacctgactg
    26101 agcatacgct tcttccaatt cttgaggact tagttcagta actcctgtag gagctacttt
    26161 taaagattga taaagctctt ctgctttagc aactgctttt tgctcaactt ttttaacttc
    26221 ttcaactgct tcttttgata tcgttaccaa accactagag atcacttttg cttgttgact
    26281 atctaggaaa atagcttgat tcttactaac ttcttttccc gtacttttaa agctactaag
    26341 tttcgcttta atagcatgca ttctttgatt cacattcgta tgaactactg aatagttttc
    26401 tttgtaagca aatttatctt ctgctaattt taaagaacca tctggattgt actggtagcc
    26461 tccccaaata tgctgattgg taaaattagc aacccagcta cctcctgaaa cagctttact
    26521 gtcaactatt ttaacaattc cgacagcatt atcgcttcct tctttaggat aacctattcc
    26581 aattttatcc ttggaatcaa cgtaattatt gaccacccct tttattttat caacctgttc
    26641 tttttgttta tctgtcaaaa tactataaag gtttggaccg ttatatatcc aagctccttg
    26701 aactcggtaa atttgattat catccaattc tgcaatagca tactgtgcat tcattgaccc
    26761 taacgagtgt ccgtagaccg aaatttttgc gttaggatat ttattcataa cttcttttag
    26821 cagatgagct gattgttcta actgctttgt accttgttta tgagatacac tttgtgaacc
    26881 aaatttcttt aataatagtt tactagctat tggaatatca tttttaaacc agtctgtttt
    26941 ccaatcatcc cctccaaacg gtgctgttga ccccttaaat aaaaccgtaa cttccttaac
    27001 atcctctggc ttgtctaaag ttccatttgg agtgatggta taaatttgtt ccccggtttc
    27061 tttgcctcca tcttgaacgt gagcaacttt accgatgggg agtaccttat cttggctcat
    27121 tattttgact agtgtatcaa gttggctacc taattcatac tcttgcttcg ctatatttac
    27181 cctatctaaa tcagtcaaga ctttttccat taatgacctc tactttccaa taaatgagtt
    27241 aactggggag attgatggct actttgactg accaaatgcc cttgattatc cctatccaaa
    27301 ttacaagtaa tatataagtt aggatcttta ttaatgatta accttaccga aatccctccc
    27361 ataggatttt ttttgatact ttctttgtca atatcatacg acttaataac agcattctct
    27421 ttgaaagcat cttcatcttc tgcataaatc cacttttgat aagcattttt agcgtctttg
    27481 ctattagcta tttcccataa ttcttgttgt ttattgtttt cagtcacttt taaacctcct
    27541 acagtgaaac caagccctaa aaggattatt acaataaaac taagtaattt tattgacttc
    27601 atctctcctc cttttatcat ataacactca tttgatatct agtttatcat aaatttattc
    27661 tcattataaa tgaaaaacta taaaacatgg attagttatc ttaggattac caaatacaaa
    27721 gatattaaaa aagatgatta atagctctaa tgctataaat catctgactc tactatttac
    27781 ttgtaatcac ttggacgtcc tgtatatttt gcccaagatt tcttcaaaaa tttattatta
    27841 atgtgataat tcggcttgct gttttcctct gttaaaaatg gattgatatg cttatctagc
    27901 tctaaccaac cattccaacc taaatggatt gtatcttgca taaagtatgg cttacctcca
    27961 tctcgagaaa ggtcagcgat atgattgaaa ccttgacttt gaagttggtg ttttattttt
    28021 tctacagatt tttgatacat tttttgatca agccctgtgt agtctgtcca ctttttattt
    28081 actggtggta tgacaaaaag gacatctgta ttttgttttg agaattcatt aagaaccaac
    28141 tgtaaatcat taaattctgg tgacttagta taattgaaat gtcgttgtga ccctttcaat
    28201 ttttttaagt gctttttaat tcttgtttgg taaaagcgat catcaatacc aaattgattt
    28261 gatctcgttg ctactttagc atcttgtgat gcaatacgcg ataaatgatt atatgagaat
    28321 gctttcggta atttaccaac atgaggcatt attgcctgat tataagtttt tcctagtggt
    28381 agatagctaa aaatagcatc tgtcttctca aatacccaat cattaaactt tagtctttgt
    28441 ttgtctttat ttgatagttt ttggccatct gcaacttttt ctatcaaatc tgacatagaa
    28501 gcttcaggat ataattttaa caatcgacga gcagcatacc tatcgtaggt tgtccctgtt
    28561 tgattttgca aaaatcgaat ggtttgatcg ttgctgaaat agttttgaaa agcacctttg
    28621 ttggctccct tgcgaacaaa ccattgcgga gaaataacat agatagcttt tttatttttg
    28681 atttgtcctt taatctgttg aatgccatag tattgtgtca gagaagccgc ccctttttga
    28741 cctaaaagat atgggatata agacctattg taagcctctg ctaaaactga tggatgcatt
    28801 gcgtcaaatc ttagccactc acttgaacca aagaaaggga caaaattggc tttaggatca
    28861 ctgaaagctc taactttttg aactctgctt ttaaaacctg ccttcgtcaa cgctactgca
    28921 ctgttttttt ccttacctaa atgatgaggc tgagaaacag gaaaaacaaa atataatcct
    28981 accaacaata gtaaagcaca aactagaggt ccaaatactt tacctagacg tttaagcatt
    29041 gcagagctcc gttactccag caacaatttt attagctgtg ttccagtcat tacgaccaaa
    29101 ttccgcaatc ggaatatcaa tattaaagtg gctttccaac tcaacaatta gttcaacagt
    29161 acccatgctg tctagaactc ctgcgtcaaa taagtcttca tccatcatag aagaaacatc
    29221 ctccataaat aggtcatcaa taattgctaa tacttctgat ttaatatcca ttataatact
    29281 ccttattatt tgttatgcgg ttgtgggaac cacaaaagat ctaaaaatcc tgaaaaaatc
    29341 aagaaagaaa acataactac attaaaggtg atgaagacac ccaaagcata agtccatttg
    29401 ttttctggta aaagtggttt tttagccaat cttctttctt tattaatatt tttcttctta
    29461 cgtacccaag cgtcattaat aactaggcca atcccatgaa agagaccata ggctatatag
    29521 taccaagtta acccatgcca gaatcccata agaagcatat tgataatata agctacactt
    29581 gaagtaacat tacggttttt aaaaacttta tttttaacta aaagcttaac aagcctcatg
    29641 aaaacaaagt ctctaaacca gaaagaaagg ctcatatgcc atctattcca aaattctttt
    29701 aggtcgcgtg atttgaaagg tttgtcaaag ttaatcggag acttaatccc cattaggtta
    29761 gaaatagcca acgcaaacat tgtgtaacct gcaaaatcaa agaacaaatc aaaaccaaag
    29821 gcatacataa ccccaagtgt tggccaatta aacacaccac cctgttctag cgctaattct
    29881 tttagaggag gcataatcag acttccaaaa atataggcta aaataaactt atagagaaaa
    29941 cctaacatca aataatgaat cgcttgacct aacatatcta ggagttcgtt tcgatctggg
    30001 atattaatgt aatcctcatt gaatcgttta aaacgatcaa ttggtccact tgagaaagtt
    30061 ggaaagaaga gtaaaaatct taagaattcc cataaagtaa attctttcaa gacaccgtct
    30121 ctcatttcaa taatcatagc aacagctcta aaagtaaggt aagaaattcc tagaaaccca
    30181 aaaagtgact gatgcccatc ctcagatata gctgaaatta attttaccaa aaatagcgga
    30241 aaaatagata aacaaaccgt tacataaaag accaaagtat gattaaatcg tttccggtaa
    30301 gctttataga taaaaactag cagagactgt ccgacaacgt atgccaatag tgacttcaat
    30361 tgattaaaag tactaccagt caacatcatt acaataaaac ttagactaaa aataatctca
    30421 tataaggcaa aacgcttctt aaagaataag ccgatgtaaa ttggtaggac agctaagata
    30481 aggtagaaga aatattgtgg attcccataa acatccaagt gaggaagttt ttctaaaaat
    30541 gcctgtatca cttgtttacc tcgctcataa gacctttgat atcaattttc ccatttggtg
    30601 tcaaaggtaa atcctctcga tagataaatt tagatggcat catgtaatcc atcataatgt
    30661 cctttaagtc ttccttaatt gcttttgtca aatccaaatc acgttcaaaa tcatctctta
    30721 caccttcttt taagacaata taggctaata agttttgaac tttatgatcc ttgttataac
    30781 gtggcactgc tactgctgat tttacatact gcgatttgtt taagttttga gaaacatctt
    30841 ctaattcaat acgatagccg ttaaatttaa tttggaaatc catacgccca ccgtaaagca
    30901 gaagaccttc atcggtcata cttcctaagt caccggtatg gtaagcaggt agaccttcga
    30961 attggaaaaa tgcctcagct gtcttttctg gattgttaag atagcctttt gatactgctg
    31021 gtccagcaat aatgatttca ccttgctctc cgtttggtaa tttatgacct tcttcatcaa
    31081 tcacatacgt tggtgagtca tctttagtgt aaccaattgg aaggcgtttg catgtttcta
    31141 acatttcatc agtaattgct actgcggata gagcaactgt tgcttctgtt ggcccatatg
    31201 cattaacgat acgagctttt ggaaaacgtt gacgaagttt ttgtgcagtc ttgacagtta
    31261 actcttcccc atcaaaataa aaatgtgtta actgtggcaa ggtctctgaa ttgaaatcgt
    31321 tagatagtag cgccatatca gcaaatgatg gtgtcgaagt ccaaacctgt attggcaatt
    31381 cattaatggt agcgaatagt tttttgaaat cattaactac tgtctttggt agggcaaaca
    31441 gggtgcctcc catagctagt gttggtgccc aatacataac tgataagtca aatgaatatg
    31501 gcggttgagc caacatttgc ggtctttcag gaactgaaaa ctcatcatca gaaatcatcc
    31561 aatttgtaaa gctcaataaa ttgtcatgtg aaatttgcac accttttggt aaaccagtag
    31621 tccctgaagt gaaaataata tagtaattat catcaccttt aacagaatgt gttacctcat
    31681 aaggagtctt ttcttcaaaa atagctgaaa cttgagaaac gtctaggatt gggacattat
    31741 caacttcaag aggaaattca ccaattgaaa tgataaggct tggttgagca actgtcataa
    31801 tagcctgtat tctatccaaa gcagagtgtt ggtcaaccgg tatataagca tgccctgact
    31861 ttgttaaagc aacaaatgtc gccaacattt catattcttg accaccgaat actaagacag
    31921 gtgatttttc aacaaggcct aggctatcaa tatgagcagc tagagagtca gagtctactt
    31981 taagttgtcc ataagtatgg acttccccta aaatatcata cactggaaaa tcagcttgtg
    32041 tctcagcaaa atgctcaatt gttttaatca tatcatgtat catttgtacc atctcctaga
    32101 attcattata aataaaactg ccttgtccgc gactaaggta tgcaaaaaag taaattagta
    32161 acaataaaat accgaagtat aataacgttc taccaataaa agataaatat ggtttcatca
    32221 ttcttccttt aattaatcat ggtattggtc caaaatccat caattggtaa ctggacttca
    32281 agacgtaatt ggtctttttc ttgatatgct ctaattctcc ccttatggcg ttccacgatt
    32341 gctttagcaa tagataagcc tagaccaaaa ctgagagaat ctgcatgatc atctttagct
    32401 tgataaaaac gctcaaagag attatccata acctctttgg aatagatagc acttggattt
    32461 gatattgtca gaatagcttt tcttcgagct tgtttaagac taaactggat tgctgatttt
    32521 gacaaagaat gcctaatagc attatccaaa agaattatta aaatttgctt aatctgactc
    32581 tcttctccaa caatccagac atcatcttca atcgtatcat ttaaacactt ttcttcttca
    32641 tatgccaaac tttcatatgt tagaagtact tcttgacata agacaaataa gtttaacttt
    32701 ttgaaattta ccttggagcg tttttcatca agtctagaca aattaagcaa atcttcaatt
    32761 aaaaactcca ttcgcttagt ctccgaaaca acatgatcca gataacgatt ccctggaatt
    32821 tgctgttcca gaacctgaac gttggctcgt atagctgcaa taggggtctt cagctcatga
    32881 ctcgcatctg aaacgaactg tttttcgcga gtcatttctg tctctacagg tcctacaata
    32941 aatcgagaaa gataaagact tacagctgct aataaacaaa atccaaaaat agtaaaaaca
    33001 agaattagaa aacgccttgc ataagggact tcataactag ccaaatccat aatagctatg
    33061 gtgtaggttt tatctttata ttcttttatg gaatatataa aagacttcca ttttcgtgac
    33121 ttctttcctt gctcaaggag cttcaaacta gatttttcga taacatcatt tgtcaaatca
    33181 gtattattgc tttgaatctt aaccatcttt tggttatcta tagtcaccaa agcaatcggt
    33241 tcatcgcctg gtattcctaa ataatcattt ttggcaacta atttaacaat cctatatgca
    33301 tcatattcat cccaatattg attgtagcga ttactaactg caaatagaaa gagaacaacg
    33361 acaataagga ttgacatggt caaaaagaca aattttttgc gtaaatcact aaacattatg
    33421 tcctctcctc caaggaatat ccaaagccct tagtggttat tatttcaatg tctgctttta
    33481 aaaagcgcaa tttttttcgc aaaaaagaaa taaatacttc taactggtta tattctgaat
    33541 tgtctgaagg tccccaaacc ttagaaatca actgattttt aggaataatc tgctttgcat
    33601 ttcgcataaa aacttccatc aagagaaatt ctttattgga taattttaca ctactttctt
    33661 tagattctag ctcatgtgaa tctgtgttaa gccttatatt tccaagccgc aattgatttg
    33721 ctctgattag agaagattgt ctggttctca gcctaattct agctagcaat tcatctgctt
    33781 caaagggttt tgtgatataa tcatctgccc ctaaatctaa accattaact ttatcataag
    33841 tctgtgattt tgcagttaaa aagattattg gggtttctaa accagcttcc ctcactcttt
    33901 ttaaaacaga taatcctgag cgtttgggta acataatatc caacaggact aaatcataga
    33961 gacctgtcaa aatgtattct aacccatcat ctccatcaaa tgctaaatca acagaataac
    34021 ctttatctgc caataaagct tgaatagcct ctgctatact tttttcatcc tcaactacaa
    34081 gaagtctcat tttctagtca ccttaccata tatattatag tataaactag caaaattgaa
    34141 ataaccttga aaaaacgata ttaataaaag tatttcctaa gtcactaaac tacttcaata
    34201 atacaagcgt ctaaaaaata aaatatcact cgctataaag atatttctca ataagaaatc
    34261 tcctcatatt ttaacaaaaa taggggctat ttgtctacga atttccgcat tataaactca
    34321 cttttcatta actttatcta taatatactc aaactactct cacaaaaacc tttatcgtta
    34381 tgaaggtacc aaaaaagcca ccagactcga gtacctgttg gcttgatatt attttgctat
    34441 ctatattaag ctgataacac tttacgtcct ttacgacggc ggctagcaag cacgcgacgt
    34501 ccgtttttag ttgacatacg gtgacggaat ccgtgtttac gttggcgacg gattttactt
    34561 ggttgataag tacgtttcac gttgaatacc tcctgataga ttttcgtatt cgtttagccg
    34621 gctattttga tcagttacta aacataccat actattttat aacaatttta ggcctcttgt
    34681 caagaaatac tttcaagttc atttgtcttt tttcagtaaa tagttccatt tgttagcaag
    34741 aaatgcttct ctattttctc caactaaaag aaaagatgtg ccatcaaaac aataataggt
    34801 aatgaaataa ttgttctctc tataaaaatg ataaataatt ctagaatgtt gatagggatc
    34861 ttggaaccta atatcaccgc accagtctca gacatataaa ttaattgcac aatagaaact
    34921 gttgcgacaa taaatcttgt catctcactg tgaatttcag ccgccattat cgaaggaaca
    34981 accatatctg aaaaaccaac aatcatggtt tgactcgcaa tatctgcttc tggaatccct
    35041 aaaagttcaa ggaacggaag gaatggtgta cctaaaattt taaaaatagg tgttgatact
    35101 gaaataatca aagctattgt tccgacagtc ataattgttg gcataacgcc aaaccataaa
    35161 ctaagtactg tcttagaggc attattgaca aaagtcttca tgtttccatt tgcttttgat
    35221 ttatcgatgg caagcttaag tccccactct atcttgttat aaccatccgg aattacctca
    35281 gatgtttctt tctttggttt agtgtaataa ctatttgatt gacgagataa gggagggata
    35341 taagggataa tgaatgcaca gacaacactg ctaatgatga ctgttagata aaaatggcca
    35401 aaataatcag ttaagccaac attctgcaaa acaacaagac aaaaagtgat tgatactgct
    35461 gaaaaagtag tggaaatcgt tgcagcttct ctactggtat aataaccctc aacatactgc
    35521 ttatcggtca aagcaattcc aatagtacca tcacctacaa atgaggctac acaattaact
    35581 gctgaccgac caggtagctg aaagaatgga cgcataatgg gagttaggaa gacaccaaca
    35641 aactccagta gaccaaattc tgtcaaaaat ggaagaataa aacccgcagc aaggaaaatg
    35701 gtaaacaaac caccaatcaa atcaaaaagg ataaggccgc ctgtctcttt cccccatacc
    35761 atttcaggac caatatgtaa aaccgtcatc agaccaagtg ccaaaccgat caaacgaata
    35821 atcagccaga aaatggtaat gtcagaaatt tcttttagca agtcattttt ttcaataaag
    35881 ctaggtctaa acagacgata acaaagtgct aacacacatg aaataaaaat gattagtaaa
    35941 ataatataat gtataggaaa aacagcgtta atccattgat taattgtctt agagattaca
    36001 gagacagcta ctgttgtcat accattatat ttaaatggag tcattaataa taatactcca
    36061 aataacgacg gaataataaa ccacaagata tctttaatag atatactttc ttttttcata
    36121 caatcctcct ggataatatg gaattgtttc taaatatact cataacttcc attgtaaaat
    36181 tttataataa aaaaaacaac cttgaattaa agaaaatttt acttagtaat actttagtaa
    36241 tttctcaaag taagttccgt ctgccctcca aaaccggaag ttagtgtgaa aaggattttt
    36301 atggtggaat taagtctgag acgtttgggt tagaaatgag tttatccatc atgacaaaac
    36361 acaaacacct aactctctta gaccgtaatg acatccaatc tggtttggat agaggagaaa
    36421 cctttaaagc catcgggctt aatctactga aacaccctac tactatcgca aaagaagtca
    36481 aacgaaataa gcaactaaga gaatcaacca aagactgcct agactgtccg ctactaagaa
    36541 aagctcccta tgtttgtaat
//
LOCUS       DATRMA010000013        36450 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.013, whole genome shotgun sequence.
ACCESSION   DATRMA010000013 DATRMA010000000
VERSION     DATRMA010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 36450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36450
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.013"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            complement(559..1197)
                     /locus_tag="VBC28_000608"
     CDS             complement(559..1197)
                     /locus_tag="VBC28_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000597402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEO6814610.1"
                     /translation="MIKSKILLQLMQGKIVAVIRGNSQEEAFQAAQACIKGGISAIEI
                     AYTNSKASQVIEQLVTQYTNQEQVVVGAGTVLDSETARMAILAGAKFIVSPAFNLQTA
                     KLCNRYAIPYLPGCMTLSEVTTALEAGCEIIKIFPGGTLGTSFISSLKAPLPQVQIMV
                     TGGVNLTNAKDWFLSGVTAIGIGGEFNKLAALGEFDKITEMAKQYKIQLNLN"
     gene            complement(1209..2216)
                     /locus_tag="VBC28_000609"
     CDS             complement(1209..2216)
                     /locus_tag="VBC28_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEO6814611.1"
                     /translation="MSKILFFGEPLIRITPKENDYFADSISTKLFYGGSEVNTARALQ
                     GFGQDTKLLSALPNNPIGNSFLQFLKAQGIDTHSIQWVGERVGLYFLEDSFACRKGEV
                     VYDRDHSSLHDFRINQIDFDQLFEGVSLFHFSGITLSLDESIQEITLLLLKEAKKREI
                     TISLDLNFRSKLISPKNAKILFSQFATFADICFGIEPLMVDSQDTTFFNRDEATIEDV
                     KERMISLINHFDFQVIFHTKRLQDEWGRNHYQAYIANRKQEFVTSKEITTAVNQRIGS
                     GDAFVAGALYQLLQHSDSKTVIDFAVASASLKCALEGDNMFETVTAVNKVLNQSKDII
                     R"
     gene            complement(2242..2880)
                     /gene="dhuI"
                     /locus_tag="VBC28_000610"
     CDS             complement(2242..2880)
                     /gene="dhuI"
                     /locus_tag="VBC28_000610"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase DhuI"
                     /protein_id="HEO6814612.1"
                     /translation="MKIALINENSQASKNTIIYKELKAVSDEKGFEVFNYGMYGKEEE
                     SQLTYVQNGLLTAILLNSGAADFVITGCGTGIGAMLACNSFPGVVCGFAADPVDAYLF
                     SQVNGGNALSLPFAKGFGWGAELNLRYLFERLFEDEKGGGYPKERAVPEQRNARILSE
                     IKQITYRDLLSVLKEIDQDFLKETISGEHFQEYFFANCQNQNIADYLKSVLD"
     gene            complement(2897..3709)
                     /gene="dhuD"
                     /locus_tag="VBC28_000611"
     CDS             complement(2897..3709)
                     /gene="dhuD"
                     /locus_tag="VBC28_000611"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000139169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric 2-dehydro-3-deoxy-D-gluconate
                     5-dehydrogenase DhuD"
                     /protein_id="HEO6814613.1"
                     /translation="MTEQFLKDNFSLEGKVALITGASYGIGFSIATAFARAGATIVFN
                     DIKQELVDKGISAYKKLGIKAHGYVCDVTDEDGINEMVDKISQDVGVIDILVNNAGII
                     KRTPMLEMSAADFRQVIDIDLNAPFIVSKAVLPGMIQKGHGKIINICSMMSELGRETV
                     AAYAAAKGGLKMLTKNIASEYGSANIQCNGIGPGYIATPQTAPLRERQDDGSRHPFDQ
                     FIIAKTPAARWGEAEDLGAPAIFLASDASNFINGHILYVDGGILAYIGKQPQ"
     gene            3987..4421
                     /locus_tag="VBC28_000612"
     CDS             3987..4421
                     /locus_tag="VBC28_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEO6814614.1"
                     /translation="MIKIIIVAHGNFPDGILSSLELIAGHQEYVVGINFIAGMSSNDV
                     RVALQREVIDFKEILVLTDLLGGTPFNVSSALSVEYTDKKIKVLSGLNLSMLMEAVLS
                     RTMFEHVDDLVDKVITSSHEGIVDFSTCLATQTAEATFEGGI"
     gene            4424..5620
                     /locus_tag="VBC28_000613"
     CDS             4424..5620
                     /locus_tag="VBC28_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="unsaturated chondroitin disaccharide hydrolase"
                     /protein_id="HEO6814615.1"
                     /translation="MMKIKPVKVESIENPKRFLNSRLLTKIEVEEAIEKALKQLYINI
                     DYFGEEYPTPATFNNIYKVMDNTEWTNGFWTGCLWLAYEYNQDKKLKNIAHKNVLSFL
                     NRINNRIALDHHDLGFLYTPSCTAEYRINGDVKALEATIKAADKLMERYQEKGGFIQA
                     WGELGYKEHYRLIIDCLLNIQLLFFAYEQTGDEKYRQVAVNHFYASANNVVRDDSSAF
                     HTFYFDPETGEPLKGVTRQGYSDESSWARGQAWGIYGIPLSYRKMKDYQQIILFKGMT
                     NYFLNRLPEDKVSYWDLIFTDGSGQPRDTSATATAVCGIHEMLKYLPEVDPDKETYKY
                     AMHTMLRSLIEQYSNNELIAGRPLLLHGVYSWHSGKGVDEGNIWGDYYYLEALIRFYK
                     DWELYW"
     gene            5675..6169
                     /locus_tag="VBC28_000614"
     CDS             5675..6169
                     /locus_tag="VBC28_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HEO6814616.1"
                     /translation="MAAGPNIVMTRVDERLIHGQGQLWVKFLSCNTVIVANDDVSKDH
                     LQQTLMKTVVPESIALRFFDIQKVIDIIHKANPAQTIFIIVKDLKDVYRLVAGGVPIK
                     EINIGNIHNGEGKEQVSRSIFLGMKDKEIIRKLNQEYHIAFNTKTTPTGNDGAVEVNI
                     LDYI"
     gene            6205..7071
                     /locus_tag="VBC28_000615"
     CDS             6205..7071
                     /locus_tag="VBC28_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEO6814617.1"
                     /translation="MDISILQAVLIGLWTAFCFSGMLLGLYTNRCIVLSLGVGVILGD
                     IQTALAVGAISELAYMGFGVGAGGTVPPNPIGPGIFGTLMAITTAGTKGKITPEAALA
                     LSTPIAVGIQFLQTATYTAFAGAPETAKKALQAGNFRGFKIAANGTIWAFAGLGFGLG
                     VLGALSTQTLTDLFALIPPVLLNGLTLAGKMLPAIGFAMILSVMAKKELIPYILLGYV
                     LAVYFGLPVLTPTANGDGVLTSVATNSVLGVPTIGVAIIATIFALLDIFRKPAAPTKE
                     TKTEGDNQDDWI"
     gene            7058..7873
                     /locus_tag="VBC28_000616"
     CDS             7058..7873
                     /locus_tag="VBC28_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEO6814618.1"
                     /translation="MTGSKKLAKSDYTKTALRAFYLQNGFNYSNYQGLGYANVIYPAL
                     KKYYGDDKKALAGALEENVEFYNTNPHFLPFVTSLHLAMLDNERPEEEIRGIKMALMG
                     PLAGIGDSLSQFCLAPLFSTIAASLATDGLVMGPILFFVAMNTILTGIKLVTGMYGYR
                     LGTSFIDKLSEQMSVISRAANIVGVTVISSLAATQVKLTIPYTFAPEKVTSTTQKIVT
                     VQGMLDKIAPALLPALYTFLMFYLIKNKKWTTYKLVILTVIIGILGSWLGILA"
     gene            7953..9857
                     /locus_tag="VBC28_000617"
     CDS             7953..9857
                     /locus_tag="VBC28_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000654956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HEO6814619.1"
                     /translation="MKDRLYNKFKDFDREFCQKYIKTYQSNAYQEMKASVNLMMRNTF
                     VFNDNWDMEPCSKAYCLDPLEWDKPVTDDPEWLYMLNRQTYLFKFLVVYIVEGDKSYL
                     RQMKYFMYHWIDCQFTLKPEGAVSRTIDTGIRCMSWLKVLIFLDYFGLITETKKIKLL
                     TSLREQITYMRDYYREKDSLSNWGILQTTAILACLYYYEDELNLPEIQSFAEEELLLQ
                     IKLQILDDGSQYEQSIMYHVEVLKSLMELVILAPKYYLPLEETIEKMVTYLIAMTGPD
                     YCQLAIGDSDVTDTRDILTLATLVLKSSKTKSFSFDNVNLETLLLFGKPSIYLFEEIP
                     RATIGESAYLFPDSGHVCLRDDRRYIFFKNGPFGSAHTHSDNNSVCLYDKKKPIFIDA
                     GRYTYKEEQLRYDFKRSTSHSTCTLDGQPLEMIKDSWTYNSYPKCDYCQLTSKDRYHL
                     VEGQLHVQRASDIYYHKRWLLTLPQAITLVIDKVSCPGEHVLTNQYILDDQVIYENGF
                     VNDLKLVSPTTFNLEDCLISKRYNQLTESHKLVKKIKFVDEVMDYTLIVDRNCQVKYV
                     PLVQTNSHKELSNSIAFDIRSQDFHYLIGVLMDDIIFGDKLYLMQGIKCKGKVIVYDK
                     NNGKMSRLKN"
     gene            9937..10938
                     /locus_tag="VBC28_000618"
     CDS             9937..10938
                     /locus_tag="VBC28_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEO6814620.1"
                     /translation="MSKKMTINDIAQLSKTSKTTVSFFLNQKFEKMSDETRQRIQEVI
                     DETGYRPSTIARSLNSKKTKLLGVLIGDITNTFSNQIVKGIEHITKQKGYQIIVGNSN
                     YDAKSEEDYIENMLNLGVDGFIIQPTSNFRKYSRILKEKKKPMVFFDSQLYEHKTSWV
                     KANNYDAVYDMTQECLNRGYKKFIMITADTSLLSTRIERASGFMDALKDNGFGYDTLV
                     IEDDDHSKSDIEDFLKAVVPDKEETLVFAPNCWALPMVFTAMKNLNFDMPRVGLVGFD
                     NIEWTDFSSPKVSTIVQPAYEEGEQVAQILINRIEGDDSVDNQQIVDCQMFWKESTF"
     gene            complement(11009..11623)
                     /locus_tag="VBC28_000619"
     CDS             complement(11009..11623)
                     /locus_tag="VBC28_000619"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEO6814621.1"
                     /translation="MSAIDKLVKASHLIDMNDIIREGNPTLRKVAEEVTFPLSEKEEI
                     LGEKMMQFLKHSQDPIMAEKLGLRGGVGLAAPQLDISKRIIAVLVPNVEDAQGNPPKE
                     AYSLQEVMYNPKVVSHSVQDAALSDGEGCLSVDREVPGYVVRHARVTIEYFDKTGEKH
                     RLKLKGYNSIVVQHEIDHIDGIMFYDRINEKNPFAVKEGLLILE"
     gene            complement(11689..12333)
                     /locus_tag="VBC28_000620"
     CDS             complement(11689..12333)
                     /locus_tag="VBC28_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000350701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEO6814622.1"
                     /translation="MDIENYCQDYCLETIFPKHYFKDLQVITLTAGQSVCKQGEQLEY
                     LHYIVKGRFKIVRRLFNGKEHILDIKTKPTLIGDIELLTNRQIVSSVIALEDLTVIQL
                     SLKGRKEKLLTDATFLLKLSQELAQAFHDQNIKASTNLGYTVKELLASHILAIEEQGY
                     FQLELSSLADSFGVSYRHLLRVIHDMVKEGLIQKEKPKYFIKNRFALESLNIQP"
     gene            12445..13608
                     /locus_tag="VBC28_000621"
     CDS             12445..13608
                     /locus_tag="VBC28_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO6814623.1"
                     /translation="MKIFMEKLSLLSLSLILLSTFSTSPALPQMISYYRDKGLPSPQV
                     ELLFSIPSMAIIFILLITPWLSKKLSEKHMIIFGLLLTALGGGLPVVSQNYLLVFVSR
                     LLLGSGIGFINARAISVISEYYQGKERRKLLGLRGSFEVLGNAGLTALVGLLLTFGWS
                     KSFMIYFLALPILVLYLVFAPKKVVKDTNDKIKTKGQKIPKADLTYIVALAILAGFVI
                     TINTGINLRIPLLVVEFGLGTPAQASLVLSAMMLMGIIAGMSFGQLIAMFHKQLIPIC
                     LVLFSLTLLGVGLPSNLMVLTISAMASGFLYSLMVTAVFSLVADRVEYSLVGSATTLV
                     LVFCNIGGASAAILLSCFDHLLGQINAVFYVYAILSLAVGMIYFFKRLFFKKM"
     gene            complement(13654..14637)
                     /locus_tag="VBC28_000622"
     CDS             complement(13654..14637)
                     /locus_tag="VBC28_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEO6814624.1"
                     /translation="MIEKYYNWATLGTGVIANELAQALEARGQKLYSVANRTYDKGLE
                     FATKYGIQKVYDHIDQVFEDPEVDIIYISTPHNTHISFLRKALANGKHVLCEKSITLN
                     STELKEAIDLAETNHVVLAEAMTIFHMPIYRQLKTLVDSGKLGPLKMIQMNFGSYKEY
                     DMTNRFFSRDLAGGALLDIGVYALSCIRWFMSEAPHNITSQVTFAPTGVDEQVGILLT
                     NPANEMATVSLSLHAKQPKRATIAYDKGYIELFEYPRGQKAVITYTEDGHQDILEAGK
                     TENALQYEVADMEEAVSGKTNHMYLNYTKDVMDIMTQLRQEWGFTYPEEEK"
     gene            14785..16680
                     /locus_tag="VBC28_000623"
     CDS             14785..16680
                     /locus_tag="VBC28_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase"
                     /protein_id="HEO6814625.1"
                     /translation="MIKYQDDFYQAVNGEWAKTAVIPDDKPRTGGFSDLADDIEALML
                     STTDKWLADENKPSDTILNHFIAFHKMTADYQKREEVGVSPVLPLIEEYKGLQSFSEF
                     ASKVAEYELEGKPNEFPFGVAPDFMNAQLNVLWAEAPGIILPDTTYYSEDNEKGKELL
                     AFWRKSQEDLLPLFGLSEQEIKDILDKVLALDAKLAQYVLSREESSEYVKLYHPYNWE
                     DFTKLAPELPLDAIFQKILGQKPDKVIVPEERFWTEFASDYYSESNWELLKADLILSA
                     ANAYNAYLTDDIRIKSGVYSRALSGTPQAMDKKKAAYYLASGPYNQALGLWYAGEKFS
                     PEAKADVEHKIATMIDVYKSRLEKADWLAQSTREKAIMKLNVITPHIGYPEKLPETYT
                     KKIIDPKLSLVENATNLDKISIAYGWSKWNKPVDRSEWHMPAHMVNAYYDPQQNQIVF
                     PAAILQEPFYALEQSSSANYGGIGAVIAHEISHAFDTNGASFDEHGSLNNWWTDEDFE
                     AFKKLTDKVVEQFDGLESYGAKVNGKLTVSENVADLGGVACALEAAQRESDFSARDFF
                     INFATIWRMKARDEYMQMLASVDVHAPAQWRTNITVTNFEEFHKEFDVKDGDNMWRPV
                     EKRVIIW"
     gene            complement(16730..17683)
                     /locus_tag="VBC28_000624"
     CDS             complement(16730..17683)
                     /locus_tag="VBC28_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEO6814626.1"
                     /translation="MKKLTKGSHIRVLSPSSSIERLGGFEANLLAKEVLEKLGFQVSF
                     SKHYLENDILYSASITSRVEDLHEAFADPSVDAILATIGGFNSNELLPYLDYDLISKN
                     PKIICGYSDSTAFLNAIFAKAKIQTYMGPAYSSFKMKEGQPYQTQAWLTAMTENHYEL
                     WPSEEWSSDPWYDPSKPRQFFPTEWKIYNHGKASGTIIGGNLSTFGLLRGTPYAPKIE
                     RYVLLIEEAEESNFYEFDRNLAAILQAYPHPQAILMGRFPKECGMTPQVFEYILSKHA
                     IFKEIPVIYDMDFAHTQPLLTVTIGAELSVDTTTLSLSIKE"
     gene            17850..18086
                     /locus_tag="VBC28_000625"
     CDS             17850..18086
                     /locus_tag="VBC28_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6814627.1"
                     /translation="MSQLSLEEQLNRLKDKYLSSKNQEFSNVHLSKVKKKKKKLIILE
                     KERCRCRLEREDTKKIDDKIEKLKKELIDVMKEE"
     gene            18088..20157
                     /locus_tag="VBC28_000626"
     CDS             18088..20157
                     /locus_tag="VBC28_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HEO6814628.1"
                     /translation="MEETILIVSFLLFLILSNVINRIFPKLPLPFIQLVFGILSGLVF
                     HKSQVHIDPELFLAFVIAPLNFREGQESDIGSFIKYRAIILYLILPTVFLTAIVVGYV
                     AGHLLPVSLPLAACFALGAALGPTDAVAFISIAKRFQFPKRAENILKLEGLLNDASGL
                     VSFQFALTALVTGYFSLAKASLKLALAIMGGFLIGLLFAFLMRLCLTVLEKFDAADVT
                     GALLLELTLPFVAYFVADLLGFSAIIAVVVAGVMQANRLKKVTLFDAQVDRVTSVIWE
                     TLNFILNGLVFLIFGRELTRIIGPLLTSNAYSNFDLISIVVLVTCTLFLVRFLAVSCF
                     YAWRSFKYHKSFKKYWREIQLLTFSGVKGSVSIATILLLPKHSVIGELGYSLILFTVG
                     AVTLMSFLTGLLVLPKLAPPLQVKDDYLIRLSILTKVLSVLEEDGKSSENQASFYAVI
                     DNYNSRIRHLILEQESSDIKKDLAELQLMMLSIESDGLEAAYRYGNISIKEYRIYQRY
                     LKYLERQVNRSFVSNITYSLTILMRVIRGIVHEFLTFGQGIRNRKKHFKNRKRLLAEV
                     NRQHLTDLYLSNNDLILETLEDMEGYYNATLIDFLQNERIRDAELIKSGLFVERVIAR
                     ITPDILNETLRGYYLERRTISEFERDGLISSKESQILRDNVNELESYSLRDSQSSIPY
                     QLLDLKK"
     gene            complement(20229..20299)
                     /locus_tag="VBC28_000627"
     tRNA            complement(20229..20299)
                     /locus_tag="VBC28_000627"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20265..20267),aa:Cys,seq:gca)
     gene            complement(20434..21966)
                     /gene="ahpF"
                     /locus_tag="VBC28_000628"
     CDS             complement(20434..21966)
                     /gene="ahpF"
                     /locus_tag="VBC28_000628"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HEO6814629.1"
                     /translation="MVLDKEIKAQLAQYLDLLESDIVLQADLGDNDNSQKVKDFLDEI
                     VAMSDRISLESTHLKRQPSFGIAKKGHESRVIFSGLPMGHEFTSFILALLQVSGRAPK
                     VDEDIIKRIKGIEKTINLETYVSLTCHNCPDVVQAFNIMAVLNPNITHTMIEGGMYQD
                     EVKSKGIMSVPTVYKDQEEFTSGRATIEQLLEQLDGPLDAEAFADKGVYDVLVIGGGP
                     AGNSAAIYAARKGLKTGILAETFGGQVIETVGIENMIGTLYTEGPKLMAQIEEHTKSY
                     DIDIIKSQLATGIEKKELVEVTLANGAILQAKTAILALGAKWRNINVPGEEEFRNKGV
                     TYCPHCDGPLFEGKDVAVIGGGNSGMEAALDLAGVTKHVTVLEFLPELKADQVLQERA
                     AKTDNLTILKNVATKDIVGEDHVTGLNYTDRDTNEEKHIDLEGVFVQIGLVPSTSWLK
                     DSGIELNERQEIVVDKFGSTNIPGIFAAGDCTDAAYKQIIISMGSGATAAIGAFDYLI
                     RQ"
     gene            complement(21984..22544)
                     /gene="ahpC"
                     /locus_tag="VBC28_000629"
     CDS             complement(21984..22544)
                     /gene="ahpC"
                     /locus_tag="VBC28_000629"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002273221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEO6814630.1"
                     /translation="MSLVGKEIIEFSAQAYHDGKFITVTNEDVKGKWAVFCFYPADFS
                     FVCPTELGDLQEQYETLKSLDVEVYSVSTDTHFVHKAWHDDSDVVGTITYPMIGDPSH
                     LISQGFDVLGQDGLAQRGTFIIDPDGVIQMMEINADGIGRDASTLIDKVRAAQYIRQH
                     TGEVCPAKWKEGAETLTPSLDLVGKI"
     gene            22821..23591
                     /gene="rpsB"
                     /locus_tag="VBC28_000630"
     CDS             22821..23591
                     /gene="rpsB"
                     /locus_tag="VBC28_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEO6814631.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIHVIDLQ
                     QTVKLADQAYEFVRDAAANDAVILFVGTKKQAAEAVAEEAKRAGQYFINHRWLGGTLT
                     NWGTIQKRIARLKEIKRMEEEGTFELLPKKEVALLNKQRARLEKFLGGIEDMPRIPDV
                     MYVVDPHKEQIAVKEAKKLGIPVVAMVDTNADPDDIDVIIPANDDAIRAVKLITSKLA
                     DAVIEGRQGEDADVDFAQEAQADSIEEIVEVVEGSNND"
     gene            23685..24725
                     /locus_tag="VBC28_000631"
     CDS             23685..24725
                     /locus_tag="VBC28_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HEO6814632.1"
                     /translation="MAEITAKLVKELREKSGAGVMDAKKALVETDGDLDKAIELLREK
                     GMAKAAKKADRVAAEGLTGVYVDGNVAAVIEVNAETDFVAKNDQFVTLVNETAKVIAE
                     GRPSNNEEALALTMPSGETLEQAFVTATATIGEKISFRRFALVEKTDEQHFGAYQHNG
                     GRIGVITVVEGGDDALAKQVSMHVAAMKPTVLSYTELDAQFVHDELAQLNHKIEQDNE
                     SRAMVNKPALPFLKYGSKAQLTDEVIAQAEEDIKAELAAEGKPEKIWDKIVPGKMDRF
                     MLDNTKVDQEYTLLAQVYIMDDSKTVEAYLESVNAKAVAFVRFEVGEGIEKASNDFEA
                     EVAATMAAALEK"
     gene            complement(24884..25345)
                     /locus_tag="VBC28_000632"
     CDS             complement(24884..25345)
                     /locus_tag="VBC28_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small multi-drug export protein"
                     /protein_id="HEO6814633.1"
                     /translation="MNYIITFLISMIPLVELRGAVPFAIANGIPLWEALAIGVVGNML
                     PVPIIFFFARKVLEWGADKPYTGKFFTWCLKKGHSGGQKLEKVAGEKGLFIALLLFVG
                     IPLPGTGAWTGTLAASLLDWEFKHSVIAVMLGVILAGCIMGTLSIIGFNLF"
     gene            25518..25982
                     /locus_tag="VBC28_000633"
     CDS             25518..25982
                     /locus_tag="VBC28_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HEO6814634.1"
                     /translation="MAIKNTSDNIEEYIKSLLEQSGIAEIKRSNLADTFQVVPSQINY
                     VIKTRFTESRGYVVESKRGGGGYIRIAKVHFSDQHQLFGNMLSTIGERISEQVFDDLI
                     QLLFDEEIITEREGNLILATSGDDVLGEQASVIRARMLRKLLQRLDRKGFHL"
     gene            25979..28426
                     /locus_tag="VBC28_000634"
     CDS             25979..28426
                     /locus_tag="VBC28_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEO6814635.1"
                     /translation="MSHYSIKLQEVFRLAQFQAARYESHYLESWHLLLAMVLVHDSVA
                     GLTFAEYESEVAIEEYEAATILALGRAPKEEITNYQFLEQSPALKKILKLAENISIVV
                     GAEDVGTEHVLLAMLVNKDLLATRILELVGFRGQDDGESVRMVDLRKALERHAGFTKD
                     DIKAIYELRNPKKAKSGASFSDMMKPPSTAGDLADFTRDLSQMAVDGEIEPVIGRDKE
                     ISRMVQVLSRKTKNNPVLVGDAGVGKTALAYGLAQRIANGNIPYELRDMRVLELDMMS
                     VVAGTRFRGDFEERMNQIIADIEEDGHIILFIDELHTIMGSGSGIDSTLDAANILKPA
                     LARGTLRTVGATTQEEYQKHIEKDAALSRRFAKVLVEEPNLEDAYEILLGLKPAYEAF
                     HNVTISDEAVMTAVKVAHRYLTSKNLPDSAIDLLDEASATVQMMIKKNAPSLLTEVDQ
                     AILDDDMKSASKALKASYKGKKRKPIAVTEDHIMATLSRLSGIPVEKLTQADSKKYLN
                     LEKELHKRVIGQDDAVTAISRAIRRNQSGIRTGKRPIGSFMFLGPTGVGKTELAKALA
                     EVLFDDESALIRFDMSEYMEKFAASHLNGAPPGYVGYDEGGELTEKVRNKPYSVLLFD
                     EVEKAHPDIFNVLLQVLDDGVLTDSRGRKVDFSNTIIIMTSNLGATALRDDKTVGFGA
                     KDISHDYTAMQKRIMEELKKAYRPEFINRIDEKVVFHSLSQDNMREVVKIMVKPLILA
                     LKDKGMDLKFQPSALKHLAEDGYDIEMGARPLRRTIQTQVEDHLSELLLANQVKEGQV
                     IKIGVSKGKLKFDIAKS"
     gene            complement(28646..29137)
                     /locus_tag="VBC28_000635"
     CDS             complement(28646..29137)
                     /locus_tag="VBC28_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEO6814636.1"
                     /translation="MIIRPVLPSDAKELLAIYAPYVADTTITFEYDIPSISEFTNRIK
                     NISKSFPYLVAEENGKILGYAYASTYYARAAYDWTCELSIYLDKDARSKKIGSQLYDT
                     LEKELVNQGYVNLLACISLPNDISIAFHKKRGFNQVAHFPKIGFKFEQWHDIVWLQKR
                     LKS"
     gene            complement(29151..29792)
                     /locus_tag="VBC28_000636"
     CDS             complement(29151..29792)
                     /locus_tag="VBC28_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019775268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEO6814637.1"
                     /translation="MLIVLAGTIGAGKSSLAAALGQHLGTDVFYEAVDNNPVLDLYYQ
                     DPQKYAFLLQILFLNKRFQSIKEAYKANNNVLDRSIFEDELFLTLNYKNGNVTKTELD
                     IYKELLANMLEELEGMPKKRPDLLVYIDVSFDKMLERIDKRGRSFEQVDSNPELYDYY
                     KQVHSEYPEWYENYDVSPKIRIDGNKLDFVKNPEDLQHVLDTIDSELQKLDLL"
     gene            complement(29913..30890)
                     /gene="dusB"
                     /locus_tag="VBC28_000637"
     CDS             complement(29913..30890)
                     /gene="dusB"
                     /locus_tag="VBC28_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEO6814638.1"
                     /translation="MTQLNSSFMIGKVEIPHRTVLAPMAGITNSAFRTIAKEFGAGLV
                     VMEMISEKGLLYNNEKTLHMLHIDENEHPMSIQLFGGDAEGLKRAADFIQSNTKADIV
                     DINMGCPVNKVVKNEAGAKWLRDPEKIYHIVKEVTSVLDIPLTVKMRTGWSDSSNAIE
                     NALAAESAGVSALAMHGRTREQMYTGTCDHETLGKVAKAVTSIPFIANGDIRTVHDAK
                     FMIEEIGADAIMVGRGARSNPYIFTQINHFFETGEILPDLPFEKMLDVAEDHLTRLVN
                     LKGETIAVREFRGLAPHYLRGKSGAAKIRGAVSRAETLAEVQELFAGLR"
     gene            complement(30874..31749)
                     /gene="hslO"
                     /locus_tag="VBC28_000638"
     CDS             complement(30874..31749)
                     /gene="hslO"
                     /locus_tag="VBC28_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEO6814639.1"
                     /translation="MDKIIKSISTSGSFRAYVLDCTETVRTAQEKHQTLSSSTVALGR
                     TLIANQILAANQKGNSKVTVKVIGDSSFGHIISVADTKGNVKGYIQNTGVDIKKTATG
                     EVLVGPFMGNGHFVVITDYATGQPYTSTTPLITGEIGEDFAYYLTESEQTPSAVGLNV
                     LLDDEDKVKVAGGFMLQVLPGASDEEISRYEKRIQEMPSISSLLESENHIESLLSAIY
                     GEDDYKRLSEDSLAFYCDCSKERFEAALLTLGTKELQAMKDEDKGVEITCQFCNQTYY
                     FTEEDLEKIINDSIK"
     gene            complement(31889..33145)
                     /locus_tag="VBC28_000639"
     CDS             complement(31889..33145)
                     /locus_tag="VBC28_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HEO6814640.1"
                     /translation="MSDTFNFDIDQIADNAITKTDKTTEIISNQTTSQTGQIAFFEKL
                     TPAQKSAISEKTPALVDTFVGDQNALLDFGQSAVEGVNTTVNHILSEQKKIQIPQVDD
                     LLKNANRELNGFIAKYKDATPAELEKKPNLIQKLFKQSKTSLQEFYFDSQNIEQKMDM
                     MAANVVKQEDTLARNIVSAEMLIEDNTKSIENLVGVIAFIESSQAEAANRASHLQQEI
                     LALDSQTSEYQIKSNQLARMTEVINTLEQQHPEYVSRLYVAWATTPQMRNLVKVSSDM
                     RQKLGMLRRNTIPTMKLSIAQLGMMQQSVKSGVTADAIVNANNAALQMLAETSKEAIP
                     MLEKTAQSPTVSIKSVTALAESLVAQNNGIIAAIDKGRKERAQLESAVIKSAETINDS
                     VKIRDKKIVEALLNEGKSTQEKVDES"
     gene            complement(33142..33960)
                     /locus_tag="VBC28_000640"
     CDS             complement(33142..33960)
                     /locus_tag="VBC28_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6814641.1"
                     /translation="MVKKRYSKNSHNLITLLGIVVLASLISDFWSEVIATLLIIGGGY
                     CAYYVYDKKRLKRFTSNQRIEALKSDIKETDQDIRHLEILKKDNRSKEYIKLAHQILP
                     QLDLIRNEANQLQKAIEPNIYKRITKKANTFSNEINEQLIKLHASPELEPISDQEDEM
                     IRIAPELKPFYHNIQDDHFAILKKIEEADNKAELAAIHQANMKRFTDVLAGYIRIKQS
                     PKNFNNAKERLEQALQAIKKFNLDLDETLRQLNESDMKDFDVSLRMMQDERNSK"
     gene            complement(34084..36336)
                     /gene="gshAB"
                     /locus_tag="VBC28_000641"
     CDS             complement(34084..36336)
                     /gene="gshAB"
                     /locus_tag="VBC28_000641"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HEO6814642.1"
                     /translation="MIIDRLLQRSHSHLPILQATFGLERESLRIHQPTQRVAQTPHPK
                     TLGSRNYHPYIQTDYSEPQLELITPIAKDSQEAIRFLKAISDVAGRSINHDEYLWPLS
                     MPPKVREEDIQIAQLEDAFEYDYRKYLEKTYGKLIQSISGIHYNLGLGQELLTSLFEL
                     SQADNAIDFQNQLYMKLSQNFLRYRWLLTYLYGASPVAEEDFLDQKLNNPVRSLRNSH
                     LGYVNHKDIRISYTSLKDYVNDLENAVKSGQLIAEKEFYSPVRLRGSKACRNYLEKGI
                     TYLEFRTFDLNPFSPIGITQETVDTVHLFLLALLWIDSSSHIDQDIKEANRLNDLIAL
                     SHPLEKLPNQAPVSDLVDAMQSVIQHFNLSPYYQDLLESVKRQIQSPELTVAGQLLEM
                     IEGLSLETFGQRQGQIYHDYAWEAPYALKGYETMELSTQLLLFDVIQKGVNFEVLDEQ
                     DQFLKLWHNSHIEYVKNGNMTSKDNYIVPLAMANKVVTKKILDEKHFPTPFGDEFTDR
                     KEALNYFSQIQDKPIVVKPKSTNFGLGISIFKTSANLASYEKAIDIAFTEDSAILVEE
                     YIEGTEYRFFVLEGDCIAVLLRVAANVVGDGIHTISQLVKLKNQNPLRGYDHRSPLEV
                     IELGEVEQLMLEQQGYTVNSIPPEGTKIELRRNSNISTGGDSIDVTNTMDPTYKQLAA
                     EMAEAMGAWVCGVDLIIPNATQAYSKDKKNATCIELNFNPLMYMHTYCQEGPGQSITP
                     RILAKLFPEL"
BASE COUNT        11454 a         6151 c         7001 g        11844 t
ORIGIN      
        1 aaaaataaaa tcagtcaaat tagaaaggag gtttagcgtg tctaaaaaac gactattaat
       61 gatatcactc aactatcaaa acttcaaaat aacggtacac tcaaaagctg agacgcagaa
      121 aaaatacttc tgttgaaatc taataaacta taatcagaaa tgtttttaat tatgtttaaa
      181 aaatgtattt gataggagct aagttagtct gtgttgagat aggaaaagcg ggtaagcctg
      241 ataggtcatt gctaagtttt tggatataaa aatgatatca aatctattat ttgctgaaat
      301 gatatgagaa tgatgagctt cactacttat atcaatctgc gggcaagtag tatatctttg
      361 gtgaagacat ggatcagttg tcggaagtag aacggttatg tctacaggaa gattagctaa
      421 aaattatctc ggtctgataa gaaaatcgag ataatgagtc tgattaagat aaacggtact
      481 acagaaaaat ggctttataa aagttctttt cttctgtctc atgattgagg tgtgggggtg
      541 ccttgtcttt ttattgtgtt aattcagatt tagctgtatt ttgtattgtt tagccatttc
      601 tgtgatttta tcaaattccc ccaaagctgc tagtttattg aactctccac caataccaat
      661 ggctgttact ccagatagaa accagtcttt ggcatttgtg agatttaccc caccagtcac
      721 cataatttgt acttgaggta aaggtgcctt tagtgatgag atgaaagatg ttcctagagt
      781 acctcctggg aatattttaa ttatttcgca accggcttca agtgctgttg ttacctctga
      841 taaggtcatg caacctggta aataaggaat agcataacga ttacatagtt tagcagtctg
      901 tagattaaat gcgggagata caataaactt tgctcctgct aaaatagcca ttcgggcagt
      961 ttctgagtct agaacagttc ctgccccaac gacaacttgt tcttggttag tatattgtgt
     1021 gactagttgt tcaatgactt ggctagcttt gctattagta taggcgattt cgatagcaga
     1081 aataccacct tttatgcatg cttgagcagc ttgaaaagct tcttcttggc tatttccacg
     1141 aatgacagca actatttttc cttgcattaa ttgcaacaaa atctttgatt ttatcataat
     1201 tctcccttct aacgaataat gtctttcgat tgatttagta ctttgttaac tgcggtaacg
     1261 gtctcaaaca tattgtcccc ttccaaagca cattttaaag atgcactagc gacagcgaaa
     1321 tcaatgactg tcttgctgtc tgagtgctga agtagttgat atagcgctcc cgccacaaaa
     1381 gcatctccgc ttccaattct ctggtttaca gctgtggtga tttcttttga agtgacaaac
     1441 tcttgtttac gatttgctat gtaagcttgg tagtggtttc gtccccattc atcctgtaga
     1501 cgtttagtat ggaaaatgac ttgaaagtca aagtgattta taagcgaaat cattcgttct
     1561 ttgacgtcct ctattgtagc ttcatcgcga ttgaagaagg tagtgtcctg tgagtcaacc
     1621 ataagaggtt caataccaaa acaaatatcc gcaaaggtag cgaactgaga aaaaaggatt
     1681 ttggcatttt taggagaaat tagtttactg cgaaaattga gatctaagga gattgttatc
     1741 tctctttttt tagcttcttt taaaagaagc agtgttatct cttggataga ctcatccaat
     1801 gatagtgtaa taccactaaa atgaaagaga gaaactcctt caaataattg atcgaaatca
     1861 atttgattta tcctaaagtc atgtaaacta ctatgatcgc ggtcgtagac cacctcacct
     1921 tttctgcaag caaaggaatc ttctaaaaaa tataacccaa cacgttctcc aacccactga
     1981 atagaatgag tatcaatacc ctgtgctttt aaaaattgaa gaaaactatt acctatggga
     2041 ttattgggta aggctgagag gagtttagta tcttgcccaa agccttgtag agctcttgcg
     2101 gtattaactt cagagccacc atagaatagt ttagttgaga tactatctgc aaaatagtca
     2161 ttttcttttg gagtgatgcg aatgagaggt tcaccgaaaa aaagaatctt agacataaaa
     2221 gtcctttcta aaacagacgc tttagtctaa gacagatttc aagtagtctg ctatattttg
     2281 attttggcaa ttagcgaaga aatattcttg gaaatgctcc ccagaaatag tttcctttaa
     2341 gaaatcttga tcaatttctt ttaaaacaga gagtaaatct ctgtaagtaa tctgttttat
     2401 ttcgctaagg atacgtgcat tgcgttgctc aggtacagcc ctctctttag ggtatccccc
     2461 tcctttttca tcttcgaaaa gacgttcaaa taaatacctc agattcaatt cagctcccca
     2521 accaaatcct ttagcaaaag gaagggaaag agcatttcct ccgtttactt gagagaataa
     2581 gtaagcgtcg acaggatcag cagcgaatcc acagactacg ccaggaaaac tattacaagc
     2641 aagcatcgct ccaattcctg tcccacagcc tgtaataaca aaatctgcag caccgctatt
     2701 aagtaaaata gcagtcagta aaccattttg tacataagtt agctggcttt cttcttcttt
     2761 gccatacatt ccgtagttga aaacttcaaa tcctttttca tcagaaaccg ctttcaattc
     2821 tttgtagata atagtatttt tacttgcttg gctattttcg ttgattaatg caattttcat
     2881 ttatattact cctttattat tgaggttgtt taccgatgta agctaaaata ccgccatcta
     2941 catagagtat gtgaccattg ataaagtttg atgcatcact agcaaggaag atagctggtg
     3001 cacctaaatc ttctgcctca ccccagcgag cggcaggtgt tttagcgatg ataaattggt
     3061 cgaatggatg acgtgaacca tcatcttgac gttcacgaag gggagctgtt tgtggtgtgg
     3121 caatatagcc gggcccgata ccgttacatt ggatgttagc agaaccgtat tcagaagcga
     3181 tgttcttggt taacatttta agtccccctt tggcagcagc ataggcagca actgtttcgc
     3241 gtccaagctc actcatcatg gagcaaatat tgatgatttt accatgtcct ttttggatca
     3301 ttccaggtaa taccgctttt gatacgataa agggagcatt caaatcaata tcaataactt
     3361 gtcgaaaatc tgcagcgctc atctctagca taggggtgcg ttttataata cctgcgttgt
     3421 taactagaat atcaattacc ccaacatctt gagaaatttt gtccaccatt tcgttgattc
     3481 catcttcatc tgtaacatca caaacatagc catgggcctt aatgccaagt tttttatagg
     3541 cggagatgcc tttgtcaacg agttcttgct taatatcatt gaagacaata gttgctccag
     3601 ctctagcaaa agcagttgct attgaaaaac caatgccgta agaagcgcct gttattaagg
     3661 cgactttacc ttctagtgaa aagttgtctt ttaagaattg ttcagtcata atatgacctc
     3721 cgtaaaaaaa ttcaggaagt aaacctgttt acatgaatag taacaaagta aaccggtttt
     3781 gtcaactata gagttcttct tctataaaac ttggcaatat atataacaaa gatttatcta
     3841 ggttataatt cttaaagata aaaaagacat cattataaaa aatcaagtaa actgctcaaa
     3901 aaatataaaa aagatgttga cacctaaaaa tgaaagcgct ataatctgat tataaaagta
     3961 aaccggtaaa ctcaagagga gaaaatatga taaaaattat tattgtagca cacggcaact
     4021 ttccagatgg tattttaagc tctttagaac ttattgctgg gcatcaggaa tatgttgttg
     4081 gtattaactt tattgcaggc atgtcttcta atgatgttag agttgctttg caaagagagg
     4141 ttatcgattt caaagaaatt ttagtattga ctgatttgct aggtgggact cctttcaatg
     4201 tttcttccgc cttatcagta gaatatacag ataaaaagat aaaagtttta tcgggcttga
     4261 acctttcaat gttaatggaa gctgttttat ctagaacgat gtttgaacat gtagatgatc
     4321 ttgttgataa agttataaca tcatctcatg aaggaattgt tgatttttct acttgccttg
     4381 ctacacaaac tgcagaagca acttttgagg gaggcattta ataatgatga aaataaaacc
     4441 ggtcaaggtt gagtcaattg aaaatccaaa gcgtttttta aacagtagat tattaactaa
     4501 gattgaagtt gaggaagcga ttgaaaaagc cttgaagcaa ctttatatta atattgatta
     4561 ctttggtgaa gagtatccaa cgcctgcaac attcaataat atttataaag ttatggataa
     4621 cacagaatgg acaaatggtt tttggacagg gtgcttgtgg ttagcttatg agtataatca
     4681 ggataaaaag ttaaaaaaca tagcccacaa aaatgtattg tcatttctaa atcgtattaa
     4741 taatcgtata gcattagatc accacgactt aggatttctt tacacaccat cttgtacagc
     4801 agaatatcgt atcaatggtg atgttaaagc tttagaagcc actataaaag ctgcagataa
     4861 attgatggag cgctatcaag aaaaaggtgg atttattcag gcttggggag aactcgggta
     4921 taaggaacac tatcgcttaa ttatcgattg cttacttaat atccaactct tattttttgc
     4981 ttatgaacag acaggtgatg aaaagtatag acaagttgcg gtgaatcact tctacgcttc
     5041 agctaacaat gtggtgcgtg atgattcttc tgcttttcat actttttatt tcgacccaga
     5101 aactggagaa ccgttaaaag gtgtcacacg acagggttat agtgatgagt catcttgggc
     5161 aagagggcaa gcatggggca tctacggtat tccgcttagt taccggaaaa tgaaagatta
     5221 tcagcagatt atccttttta aaggtatgac aaactatttt ctaaatcgtt tacctgaaga
     5281 caaggtatcc tattgggacc ttatttttac ggatggctcg ggccagccta gagatacatc
     5341 cgcaacagca acggctgtgt gtggaattca tgagatgctt aaatatttac cagaagtaga
     5401 tcctgataaa gagacataca aatatgctat gcatacaatg cttcgtagtc tgattgaaca
     5461 gtatagtaat aatgaactta tagcaggacg tcctcttcta ttgcacggtg tgtattcgtg
     5521 gcattcaggt aaaggagtag atgaaggtaa tatttgggga gattattatt acttagaagc
     5581 cttaataaga ttctataaag actgggaact ttattggtaa gagtatttta ctaacaaaat
     5641 aaacaataaa taatagaaaa cgaggaggtc agaaatggca gcaggaccaa atattgtaat
     5701 gacacgtgtt gatgagcgtc ttattcatgg acaaggacaa ctttgggtga aatttttaag
     5761 ttgtaatact gttattgtgg caaatgatga tgtctccaaa gatcatcttc aacaaacgtt
     5821 aatgaagacg gtggtgccag aatcaatagc attgcgattt tttgatattc aaaaagttat
     5881 tgatattatt cacaaggcca acccagcaca gacgattttt ataattgtta aagatttgaa
     5941 agatgtctat agacttgtag cagggggcgt cccaattaaa gagattaata ttggaaatat
     6001 tcataatgga gaaggtaaag agcaggtaag tcgctctatc ttcttaggga tgaaggataa
     6061 agagataatt agaaaattaa accaagaata tcacattgca tttaatacta aaacaactcc
     6121 aacaggtaac gatggtgctg tggaagttaa tattttagat tatatttagt tggacaaact
     6181 tactatcata taaaggagaa aaatatggat attagtattc ttcaggctgt cttaattgga
     6241 ctgtggacgg ctttctgttt tagtggcatg ttgcttggcc tctatacaaa tcgttgtatc
     6301 gttctctctc taggcgttgg tgttatttta ggagatattc aaacagcttt ggcagttgga
     6361 gcaatatctg aactggctta catgggattt ggtgtgggag cgggagggac agttcctcca
     6421 aacccaatcg gtcctgggat ttttggaact ttgatggcta ttacaacagc tggaactaaa
     6481 ggaaaaatca caccggaagc tgctcttgca ttatcaaccc caattgcagt tggtattcaa
     6541 tttcttcaaa cagcaactta tactgctttt gcaggggcgc ctgaaacagc taaaaaagct
     6601 ttgcaagcag gtaatttccg aggatttaaa attgcagcaa atggtacaat ttgggcattc
     6661 gctggattgg gatttggttt aggagtactg ggtgctcttt caacgcaaac tttgacagat
     6721 ttatttgctc tcattccccc tgttttgctt aatggtctaa ccttagctgg gaaaatgctt
     6781 cctgcaattg gttttgcgat gattctctct gtaatggcta aaaaagaact cattccttat
     6841 atcctacttg gatatgttct agctgtttat tttggtttgc cagttctaac tccaacagca
     6901 aatggagacg gagttttaac atcagtggct actaacagtg tgcttggggt tccaactatc
     6961 ggggtagcta taattgcaac aatatttgct ttgcttgata ttttcagaaa accagctgca
     7021 cctactaaag aaactaagac agaaggagac aaccaagatg actggatcta aaaaattagc
     7081 aaaaagtgat tacactaaaa cagctttacg tgccttttat ttacaaaatg gctttaatta
     7141 cagtaactat caaggtcttg gttatgctaa tgtcatatat ccagctctca aaaaatatta
     7201 tggagatgac aaaaaagctt tagcgggagc tctagaagaa aatgtggagt tttacaatac
     7261 aaaccctcat ttcttacctt ttgtaactag cttacattta gcaatgcttg ataatgagcg
     7321 gccagaagaa gaaattcgcg gtattaagat ggcgcttatg gggcctcttg caggtatcgg
     7381 tgattcttta tcacaatttt gcttagcacc cttgttctca acgattgcag cctctttagc
     7441 gacagatggt ttggtgatgg gaccgattct gttctttgtg gctatgaata ctattttaac
     7501 gggaattaaa ttagttactg gcatgtatgg ttatcgtctt gggaccagtt ttattgataa
     7561 attgagtgaa caaatgtcag ttatctcacg ggcagctaat atcgttggtg taacggtcat
     7621 ctcaagttta gctgcaacac aagttaagct tacaatccct tacacttttg ctccagaaaa
     7681 agtaactagt acaacgcaaa agattgttac tgtccaaggt atgcttgata aaattgcacc
     7741 agctctactt ccagcactct acactttcct tatgttttac cttattaaaa ataaaaaatg
     7801 gacaacttat aagttagtta tccttacagt tattattggt atccttggta gctggttagg
     7861 tattttagct taataaaatt ttgataaata aggattatga catatcaatg acatagtcct
     7921 ttttatatcg gacgaggacg ttttgtaaaa aaatgaaaga tcgcttatat aataaattca
     7981 aggattttga tagggaattt tgtcaaaagt atattaaaac ataccaatct aatgcctatc
     8041 aagaaatgaa agcctctgtt aatttgatga tgaggaacac ttttgtattt aatgataatt
     8101 gggatatgga accttgttct aaagcatatt gtcttgatcc tttggagtgg gataagccag
     8161 taacagatga tccagaatgg ttgtatatgc taaatcgtca aacttatctt tttaaattcc
     8221 tggtggtcta cattgttgaa ggcgataagt cttaccttag acaaatgaaa tattttatgt
     8281 accattggat tgattgtcaa tttacactaa agccagaagg ggcggtaagt cgtactattg
     8341 acactgggat acgttgcatg tcatggctca aagttttgat ttttttagat tattttggat
     8401 taataacaga aactaaaaaa attaaactat taactagttt acgggagcag ataacttata
     8461 tgagggacta ttatcgtgaa aaagatagtc taagtaactg gggaatttta caaacaacag
     8521 caatattggc gtgtttatac tattatgaag atgaattaaa tctacctgaa attcagagtt
     8581 ttgctgaaga agaattatta cttcaaatca agcttcaaat tttagatgac gggagccagt
     8641 atgaacaatc aattatgtat catgtagaag tcttgaagtc cttaatggaa ctagttatcc
     8701 ttgcgcctaa atattatcta ccattagaag aaactattga aaaaatggtt acctatctaa
     8761 ttgctatgac tggtccggat tattgtcaac tagctatagg ggacagtgat gtgaccgata
     8821 ctcgtgatat tctaactttg gcaacacttg tgttgaaatc ttctaaaaca aaatcatttt
     8881 cttttgataa tgttaattta gaaactttac ttttgtttgg gaagccatca atttatcttt
     8941 ttgaagaaat accgcgtgcg acaataggag agtctgctta tctttttcca gattctggtc
     9001 atgtgtgttt acgtgatgat aggcgttata tattttttaa gaacggtcca tttggtagcg
     9061 ctcatactca tagtgataat aatagtgttt gtctctatga taaaaagaaa cctattttca
     9121 ttgatgcagg aagatatact tacaaagaag aacaactaag gtatgatttt aaacgttcga
     9181 ctagtcattc aacatgtacc cttgatgggc aacccttaga aatgatcaag gactcttgga
     9241 catacaattc ttatccaaaa tgtgactatt gtcagttgac ttcaaaggat aggtaccatt
     9301 tagtcgaagg acaactacat gtccaaagag cttctgatat ctattaccat aagcgatggt
     9361 tgttaacttt accgcaggcc attaccttag ttattgataa ggtgagttgt ccaggagagc
     9421 atgtcttaac aaatcaatat attttagatg atcaggtcat ttatgaaaat gggtttgtta
     9481 atgacttgaa attagtaagt cctacgacct ttaatctaga agattgcctt atttctaagc
     9541 ggtataatca attgacagaa agtcataaat tagttaagaa aataaaattt gttgatgagg
     9601 tgatggatta taccttgata gttgatcgga actgtcaggt gaaatatgtt cctttagtgc
     9661 aaacgaacag tcataaggaa ctaagtaata gcattgcatt tgatattagg tctcaagact
     9721 ttcattattt aattggagtg cttatggatg atattatctt tggggataaa ctctatttga
     9781 tgcaagggat aaaatgcaaa ggaaaagtca ttgtatatga taaaaataat ggcaaaatga
     9841 gtcgtttaaa aaattaagag ggctattatt atgctatact agtactatat taaccataaa
     9901 aaaataaaat tagtaaaatt agaaaggagg tttgttatgt ctaaaaaaat gaccattaat
     9961 gacatcgctc aattatcaaa aacttccaaa acaacggttt cttttttctt gaatcaaaaa
    10021 tttgaaaaaa tgtcggatga gacgaggcaa cgtattcaag aggtgattga tgaaacaggt
    10081 tatagaccaa gtacgatagc aagaagtcta aattctaaaa aaacaaaact actaggtgtc
    10141 ttaattggag atataacaaa cacattttca aatcaaattg taaaaggaat tgagcacatc
    10201 accaaacaaa aaggctatca aattattgtg ggtaatagta attacgatgc caaaagtgaa
    10261 gaagattata ttgaaaatat gttaaattta ggtgttgatg gtttcattat tcagcctaca
    10321 tcaaattttc gaaagtattc tcgtatttta aaagaaaaaa agaaaccaat ggtatttttt
    10381 gatagtcaac tttatgaaca taagacgagt tgggttaagg ctaataacta tgatgctgtt
    10441 tatgatatga cacaggagtg tctaaatcga gggtataaaa agtttattat gattactgct
    10501 gatacgagct tgcttagtac acgtattgag agagcttcgg gattcatgga tgctttaaag
    10561 gacaatggtt tcggttatga cactttggtg attgaagatg atgatcattc aaaaagtgat
    10621 atagaagact ttctaaaagc ggtagttcca gataaggagg agacattagt atttgcacca
    10681 aattgttggg ctttaccaat ggtttttact gccatgaaga atctcaattt tgatatgcca
    10741 cgagttggtt tagttgggtt tgacaatatt gaatggacag acttttcatc tcctaaagtg
    10801 tcaacaattg tccagccggc ttatgaagaa ggtgaacaag tagcacagat tttgattaat
    10861 cgtattgaag gagatgactc cgtcgataac caacagattg tagattgcca gatgttttgg
    10921 aaagagtcga ctttttaaaa aagatttctt attttatttg ctattaaaaa ataaaaccta
    10981 gagaaaatta tctctaggtt atttgtcctt attctaaaat aagtaagcct tctttgacag
    11041 cgaaaggatt tttctcattg atacggtcgt aaaacataat accatcaatg tggtcaattt
    11101 catgttgaac aacaatagag ttgtaacctt ttaatttgag tcgatgtttt tcaccagttt
    11161 tgtcgaaata ttcaatagtt acacgcgcgt ggcggacgac atagccaggt acttcacgat
    11221 ctactgatag gcatccctcc ccgtcagata aagcagcatc ttgaacagaa tgcgatacaa
    11281 ctttagggtt gtacattact tcttggaggc tataagcttc tttaggtgga tttccttgag
    11341 catcctcaac gtttggaaca aggacggcaa tgatgcgttt tgagatgtct aattgtggtg
    11401 cggctaaacc aacaccacca cgtaagccta gcttctcagc cataattgga tcttgagagt
    11461 gttttaaaaa ttgcatcatt ttttctccga gaatttcttc tttctcagaa agtgggaatg
    11521 tgacttcttc agccactttg cgaagggttg gattgccttc acggataatg tcattcatgt
    11581 caattaaatg actagctttt actaatttat caatagcaga catattttct cctaactgta
    11641 ttctctgatt tagtttagca taaaataatt aaatgataaa gctacgcttc atggctgaat
    11701 atttaatgat tctagtgcga aacgattttt gataaagtat tttggttttt ctttttggat
    11761 aaggccttct ttgaccatat cgtggataac tcttaataaa tgacgataac tgacgccaaa
    11821 cgaatcagct aaactactta actcaagttg aaagtaacct tgttcttcaa ttgctaaaat
    11881 atgacttgca agtaactctt tgacggtata ccctagatta gtagacgctt tgatgttttg
    11941 atcatgaaaa gcctgagcta gctcttgact aagttttagg aggaaagttg cgtctgttaa
    12001 cagtttctct ttccgtcctt taagtgataa ttgaatgact gtaagatcct ctaaggcaat
    12061 aacagatgat acaatttgtc tattagtcaa aagttcaata tcaccaatca gtgttggttt
    12121 tgttttaata tctaaaatat gttctttgcc gttaaagagt cgacgtacaa ttttaaaacg
    12181 tcccttgacg atgtagtgca agtattctaa ttgttctccc tgtttacaga cggattggcc
    12241 ggcagttagg gtgattactt gtaaatcctt gaaataatgt tttggaaaga tagtctccaa
    12301 acagtagtct tggcagtagt tttctatatc catgtattga ctccctattt gagatagttt
    12361 taggacatat gtcatatatt tctagtttaa tattgattat actttattta tctaaaaaaa
    12421 gacatcagaa agaggtattg tcacttgaaa atctttatgg agaagctaag tttactctct
    12481 ttatcactta tcttgctatc aactttttcg acatcaccag ccctaccaca aatgatatct
    12541 tattatagag ataagggctt accatcaccc caagttgaat tattgttttc aattccttcg
    12601 atggctatta tcttcatctt gcttatcaca ccttggttaa gtaaaaaact ttctgagaaa
    12661 catatgatta tctttgggct attgctaaca gcattagggg gaggattgcc ggttgtcagt
    12721 cagaactacc ttttagtatt tgtatcgcgt cttttattgg ggagtggtat tggttttatt
    12781 aatgctaggg ccattagtgt gattagtgaa tattatcaag gcaaagaaag aagaaaactc
    12841 ttaggattaa gggggtcctt tgaagtttta ggaaatgctg gtttgacagc tttagttggg
    12901 ctgttgttga cttttggttg gtccaaatca tttatgattt attttttagc tttaccaatc
    12961 ttagttctat atcttgtttt tgcgcctaag aaagtggtga aagatacaaa tgacaagatt
    13021 aaaactaaag gtcaaaaaat ccctaaagct gatttaactt atattgtcgc cttagctatt
    13081 ttagcaggat ttgtcataac tataaatact gggattaatc ttaggatacc tctgctagta
    13141 gtagaatttg gtttgggtac gccagctcaa gctagtcttg ttcttagtgc gatgatgctt
    13201 atgggaataa tagcaggtat gagttttggt cagttgatag ctatgtttca taaacaatta
    13261 attccaatct gcctcgtctt attttcttta acattgcttg gagttggatt gccttctaat
    13321 ctaatggtac taacaattag tgcgatggca tcaggtttcc tttacagcct gatggtcact
    13381 gctgtgttta gcctagtagc agaccgtgtt gaatattcgt tggttggttc tgcaacgaca
    13441 ttagttttag tattttgtaa tattggtgga gctagtgcgg ctattcttct gtcttgtttt
    13501 gatcatttac taggacaaat aaatgcagtt ttctatgttt atgctatttt aagtttagca
    13561 gttggtatga tttatttctt taaacgccta ttttttaaga aaatgtagtg tatattaact
    13621 aaaaagggct tgacaatcat tgtcaagctc tcttcatttt tcttcttctg ggtaggtaaa
    13681 tccccattct tgacgtagct gtgtcatgat atccataacg tctttggtat agtttaagta
    13741 catgtggtta gtttttcctg aaacggcttc ttccatatca gctacctcat attggagggc
    13801 attttcagtt ttgccagctt cgagaatatc ttgatgccca tcctcagtat aagtaataac
    13861 tgccttttgt cctcgcggat attcaaaaag ttcaatgtag cctttatcgt aagcgatagt
    13921 tgctcgttta ggttgttttg catgtaaact aaggctgaca gtcgccatct catttgcagg
    13981 attggttagt aggataccaa cttgttcatc aactcctgtt ggtgcaaatg taacttgaga
    14041 ggtaatgttg tgaggtgctt ctgacataaa ccagcgaata caagaaagtg cataaacacc
    14101 aatgtccagc aaagcaccgc ctgctaggtc acgactgaaa aaacggttag tcatatcata
    14161 ttctttataa cttccgaaat tcatttgaat catttttaac ggtcctaatt ttccactatc
    14221 aactaatgtt tttaattggc gataaattgg catatgaaaa atagtcatgg cttcagctaa
    14281 gacaacatgg ttagtttcgg ctaaatctat ggcttcttta agttcagtgc tgtttaaagt
    14341 aatagatttt tcgcaaagaa cgtgcttacc atttgctaaa gcctttcgta aaaatgagat
    14401 gtgagtattg tggggagtag agatataaat gatatccact tcagggtctt caaatacttg
    14461 atctatgtga tcataaactt tttggatacc atatttggta gcaaattcaa gtcctttgtc
    14521 gtaagttcta ttagctacag aatataattt ttgtccacgt gcttctagtg cttgggctaa
    14581 ttcgttggca ataacgccgg ttccaagcgt tgcccaatta taatattttt caatcataac
    14641 aacctccttg ttggtttata gtatagcaaa aataacaaaa tgatatttag agaaagcacc
    14701 aatatgagag tatttttaat agtcaaactc tgaattttct gttagactgt aagagattac
    14761 atatgagata ttaaggagaa aataatgata aaatatcaag atgattttta ccaagcggtt
    14821 aatggggagt gggctaagac agctgttatt cctgatgaca aaccaaggac aggtggtttt
    14881 tctgatcttg ctgatgacat tgaagcatta atgctgtcaa cgacagataa gtggctggct
    14941 gatgaaaata aaccaagtga tacgatttta aatcatttta ttgcttttca taaaatgaca
    15001 gctgattatc aaaaacgtga agaggtagga gtatcaccag tattaccatt aattgaagaa
    15061 tataaaggcc tacaatcttt ctctgagttt gcatcaaaag ttgcagaata tgaactagag
    15121 ggtaaaccaa atgaatttcc atttggtgtg gctcctgact ttatgaatgc tcaattgaat
    15181 gtactttggg ctgaagcgcc tgggattatt ttaccagata caacttatta tagtgaagat
    15241 aatgaaaaag gaaaggaact ccttgctttt tggcgtaaaa gtcaagaaga cctactgcct
    15301 ttgtttggcc tttcagaaca ggaaattaaa gatatcttgg ataaggtttt agcattggat
    15361 gcaaaattgg cacaatatgt cttatctcgc gaagagagtt cggaatatgt taaactttac
    15421 catccttata actgggaaga tttcacaaaa ttagcacctg aacttccatt agatgctatt
    15481 ttccaaaaga tattaggaca aaaaccagat aaagttattg ttcctgaaga acgtttctgg
    15541 acagaatttg catctgacta ttactcagaa tctaattggg aattattaaa ggcagatctt
    15601 attttatcag ctgcgaatgc ctataatgct tacttaactg atgatattcg tatcaaatcg
    15661 ggagtttaca gtcgagcttt atcaggcaca ccacaagcaa tggataagaa aaaagcagct
    15721 tactaccttg cttcaggacc ttacaatcaa gctctaggtt tgtggtatgc aggagaaaaa
    15781 ttctctccag aagctaaagc tgatgttgag cataagattg caacgatgat tgatgtctat
    15841 aagtcacgct tagaaaaggc ggattggctt gcacagtcaa cgcgagaaaa agctattatg
    15901 aaacttaatg ttatcacacc acatattgga taccctgaga aactaccaga gacttatact
    15961 aaaaaaatca ttgatcctaa actatcgctt gtcgaaaatg ctactaattt agacaaaatt
    16021 tcaatagcct atggttggag taaatggaat aaaccagttg atagaagtga atggcatatg
    16081 cctgcccaca tggtcaatgc gtattatgat ccacaacaaa accaaattgt cttcccagca
    16141 gccattttac aagaaccatt ttatgcttta gagcaaagtt catcagcaaa ttatggggga
    16201 attggtgcag tgattgcaca tgaaatatca cacgcttttg acactaatgg agcttcattt
    16261 gatgaacatg gaagtttgaa taattggtgg actgatgaag attttgaagc tttcaaaaaa
    16321 cttacagata aagtagttga gcagtttgat ggactggaat cttatggcgc aaaagtcaat
    16381 ggtaaactaa cagtatcaga aaatgttgca gatttaggtg gagttgcttg tgctttagaa
    16441 gctgcgcagc gtgagtcaga tttttcagcg agggatttct ttattaactt tgcgacaatt
    16501 tggcgtatga aggcacgtga tgagtatatg caaatgttag ctagtgtaga tgttcacgcc
    16561 ccagcacaat ggcgaactaa tattacagta acgaattttg aagaattcca taaagagttt
    16621 gatgttaaag atggtgataa catgtggcgt ccagttgaaa aacgtgttat tatttggtaa
    16681 ctctaaatga taaactcccg ttactcagat gagtagcagg agtatttttt tattctttta
    16741 tagatagaga taatgtagta gtgtcaacgc ttagctcagc tccaatagtc actgtcaaaa
    16801 gaggttgggt gtgcgcaaaa tccatatcat aaataacagg aatttccttg aaaatagcat
    16861 gttttgaaag gatatattca aagacttgtg gagtcatgcc gcattcttta ggaaagcgcc
    16921 ccataagaat agcttgtgga tgaggataag cttgtaaaat tgcagcaagg ttgcggtcaa
    16981 attcataaaa attactttcc tcagcctctt caatcaagag gacatagcgt tcaattttcg
    17041 gtgcataagg agtacctcga agtaggccaa acgtagagag attacctccg ataatcgtac
    17101 cggaagcttt tccgtgatta taaattttcc attctgttgg gaaaaattgg cgtggtttgc
    17161 tgggatcata ccagggatca ctagaccatt cttcactagg ccaaagttca tagtggtttt
    17221 ctgtcatggc agtaagccaa gcttgtgttt ggtaaggttg accttccttc attttgaaag
    17281 aagaataagc agggcccatg taagtttgga tttttgcttt ggcaaaaata gcattaagga
    17341 aggccgttga atctgagtaa ccacaaatta ttttgggatt cttagagatt aaatcataat
    17401 ctaaatatgg taaaagctcg ttggaattga atccgccaat ggttgctaat atggcatcaa
    17461 cagaagggtc ggcaaatgct tcatgtaaat cttctacacg gctagtaatc gaagcagaat
    17521 agaggatatc attttctaga tagtgcttag agaatgacac ttgaaatccc agcttttcta
    17581 gtacttcttt ggctaatagg ttagcctcaa aaccacccag acgctcaatg gaagaagagg
    17641 gactaagaac acgaatgtgg ctgccttttg ttaatttttt cattggttaa cctcctattt
    17701 aaattgttgt tagtctacca catatggaga taatgagcaa cgatattctt aaaatctatc
    17761 aatttaagaa atgcgtgtta tactagagat agccgatgaa agaaaaaata cttattatta
    17821 ataatttggt cattaaatag gaggtcatga tgtctcaatt atctttggaa gaacaattga
    17881 atcgactaaa agataaatac ctttcttcta aaaatcaaga attttcaaat gttcacttaa
    17941 gtaaagtaaa gaagaaaaag aagaaattaa taatacttga aaaagaaaga tgtcgctgtc
    18001 gattggagag agaagatact aaaaaaatcg atgataaaat tgagaaattg aagaaagaat
    18061 tgatagatgt tatgaaggag gaataatatg gaagaaacta ttctaatagt cagctttctc
    18121 ctgtttttga ttttatctaa tgttatcaat aggatatttc ctaaattacc attaccattt
    18181 atccagttgg tctttgggat tctctctggc ttagtttttc ataagtcaca agtccatatc
    18241 gatcctgagt tattccttgc ctttgttatt gctcctttga actttaggga aggtcaagaa
    18301 agtgatattg gtagttttat aaagtatcgg gctattattc tttacttaat cttaccaacg
    18361 gtatttttaa cagctattgt cgttggttat gtagcagggc accttttgcc agttagttta
    18421 ccacttgcgg catgctttgc gcttggagca gccttaggcc caacagatgc cgttgccttt
    18481 atatcgattg ctaagaggtt tcaatttcct aaacgggcag aaaatatcct caaattggaa
    18541 ggtcttttaa atgacgctag tggtctagtt tccttccagt ttgctttaac agcccttgta
    18601 acaggttatt tctccttagc aaaagctagt ctcaagttag cattggccat aatgggtggt
    18661 tttttaattg gcttgctttt tgcttttcta atgaggttat gtctcactgt cctagagaaa
    18721 tttgatgctg ctgatgttac aggggctcta cttttggaat taaccttacc ttttgttgct
    18781 tatttcgttg ctgatttgtt gggattttca gctattattg cggtggtagt ggcaggagta
    18841 atgcaagcaa atcgtttgaa aaaagtgact ctctttgatg ctcaagtaga tcgcgttacc
    18901 tctgttattt gggaaacctt aaattttatt ttaaatggtt tagttttctt aatatttgga
    18961 agagaactga cacgtatcat tggtcctctt ttaacaagta acgcttatag taattttgat
    19021 cttattagta ttgttgtttt agttacttgc acactttttc tagttcgctt tctagcagtt
    19081 tcttgctttt atgcttggag atctttcaaa taccataaat catttaaaaa gtattggaga
    19141 gagattcaac tgttgacttt ttcaggagtt aaaggaagtg tttccatagc aacaatactc
    19201 ttattaccca agcactcagt cataggagag ttggggtata gtttgattct tttcactgtt
    19261 ggtgcagtaa cgctaatgag ttttttaacg ggacttttag tattaccaaa gttagcgcct
    19321 cccttacagg tgaaagatga ttatttaata cgtctatcga ttttaacaaa agtattatct
    19381 gtcttagaag aagatggtaa gagttctgaa aatcaagctt cattttatgc agttatagat
    19441 aattataata gccgtattcg tcatctcatt ttagaacaag aatctagtga cattaagaag
    19501 gacttagctg aattgcaact gatgatgctt agtatagaaa gcgatggact tgaagctgct
    19561 tatcgttatg gcaatatttc tatcaaagaa tatcgtattt accaacgtta tttaaaatac
    19621 ttagaacgcc aagttaatcg tagctttgtg tcaaatatca cttatagttt aacgattctt
    19681 atgcgtgtta ttcgtggtat tgttcatgag tttttaacat ttggacaagg aatacgaaat
    19741 cggaaaaaac atttcaaaaa caggaaacgg ttgttagcag aagtcaatcg tcagcactta
    19801 acagatttgt acttatcaaa taatgacctc attttagaaa cgctagaaga tatggaaggt
    19861 tattataatg caactttgat agatttctta caaaatgaac gcattcgaga cgcagaatta
    19921 attaaatcag gtttatttgt agaacgtgtc attgcacgca taacgccaga tattttaaat
    19981 gaaaccctaa gaggatatta cttggaacgc agaacaatct ctgaatttga aagggacggt
    20041 ttaattagtt ctaaagagtc tcaaatttta agggataatg tcaatgaatt agaaagctat
    20101 tcattaagag atagtcaatc ttctatacct tatcaattat tggatttaaa aaaataattc
    20161 tgataacaaa aaaatcaacg aacaatcgtt gattttttgt tatctttgga taaaatcttt
    20221 tatatttaag gcggtagacg gatttgaacc gacgatcaag cttttgcaga gccgtgcctt
    20281 accacttggc tataccgcca taacagataa tattctatcg aaaaaaaaaa gattcgtcaa
    20341 gcctctttcg taaaatagcc aattttagcc cttaaacttc ctcatattac ccaaaaaaag
    20401 agattgatac ccattgtatc aatctctttt agattattgt ctgattaaat aatcaaatgc
    20461 gccaatggca gctgtggcac cagaccccat ggaaataatg atttgcttgt aggcagcatc
    20521 tgtacagtca cctgcagcaa aaataccagg gatatttgtt gagccaaatt tatcaacgac
    20581 aatttcttga cgttcattga gttcaatgcc gctatccttg agccaagatg tacttggtac
    20641 aagaccaatt tgtacaaaaa caccttctaa atcaatgtgt ttttcttcgt ttgtatcacg
    20701 atcggtatag tttaaaccag tgacatgatc ttcaccaacg atatctttag tagcaacatt
    20761 cttaagaata gttaaattgt cagttttagc tgctcgttct tgcagtactt ggtctgcttt
    20821 gagctcaggt aagaattcta ggactgtaac atgtttagtg acaccggcta gatctaaagc
    20881 agcttccata cctgaattac caccacctat aacagcaaca tcttttcctt cgaaaagagg
    20941 gccatcacaa tgtggacagt aagtaacacc tttattgcgg aattcttcct cgccaggaac
    21001 gttgatattt cgccacttag cacccagagc taggatagca gttttagctt gtaaaatagc
    21061 tccgttagcc agtgttacct caactaattc ttttttctca atgccagttg ctaattgact
    21121 cttgataata tcaatatcgt aagatttggt atgctcttca atttgagcca ttaatttagg
    21181 gccttctgtg tacagtgtac caatcatgtt ctcaatacca actgtttcga taacttgtcc
    21241 accaaaagtt tctgctaaaa tacctgtttt taacccttta cgggcggcat aaattgcagc
    21301 actgttacct gcaggaccac caccaataac tagaacatca tagacacctt tgtcagcaaa
    21361 tgcttcagca tcaagaggtc catctagttg ttctaaaagc tgttcaattg tagcacggcc
    21421 agaagtaaat tcttcttggt ccttataaac agtaggaaca gacatgatac cttttgattt
    21481 gacttcgtct tgatacatgc ctccttcaat catagtatga gtgatattcg gattcaaaac
    21541 agccattata ttaaaggctt gaacgacatc tggacaatta tggcaagtta atgaaacata
    21601 agtttctaga ttgatagttt tctcaatccc cttaattcgt ttgataatat cttcatcaac
    21661 ttttggtgcg cgaccagaga cttgcaacaa agctaaaatg aaagatgtga attcatgccc
    21721 cattggcaag cctgaaaaaa taacgcgact ttcatgccct ttttttgcaa ttccaaaact
    21781 aggttgacgt tttaaatgtg ttgattcaag gctgatgcga tcagacatag cgacaatttc
    21841 atctagaaaa tcctttactt tttgagaatt atcgttatct cctaaatcag cttgtaatac
    21901 aatatcagat tctagcaaat ctaaatattg cgctaattga gccttgattt ctttatctaa
    21961 gaccatggat tacctcctgg ttattaaatt ttacctacaa ggtctaaact aggagttaaa
    22021 gtttcagctc cttctttcca tttagctgga caaacttcac ctgtatgttg gcggatgtat
    22081 tgagcagcac ggactttatc aatgagagta cttgcatcac gtccgatgcc atcagcatta
    22141 atttccatca tttggatgac accgtctgga tcaatgataa atgtaccacg ttgtgctaga
    22201 ccatcttgtc caaggacatc aaaaccttgt gaaataaggt gtgaagggtc accaatcata
    22261 ggatatgtga tagtaccaac gacatctgag tcatcatgcc atgctttatg aacaaaatga
    22321 gtatcagtag agacagaata aacttctaca tcaagagatt taagtgtttc gtattgctct
    22381 tgaaggtcac caagttcagt tgggcaaaca aatgaaaagt ctgctgggta gaaacaaaaa
    22441 actgcccatt ttcctttaac gtcttcattt gtaacagtga tgaattttcc atcgtgataa
    22501 gcttgagctg aaaattcaat aatttctttt ccgactaatg acataataat gtcctccttt
    22561 attttttaat agcctaatta taaacgaaat acttttaaat ctccaataaa aacgtttaca
    22621 tttttctaag aaaagtatga tttttctaat agagtagaag attttatagc ttgtcaaatc
    22681 taactttttt tgataaacta ttatagttgc ttaaagtgac aaatactcat cttagatcaa
    22741 ttgtggtctt gttgccgaaa ggttaggctg caagtcgaag tctgagagag gaaaaaacat
    22801 ttttaaagga gaacttactc atggcagtaa tttcaatgaa acaacttctt gaggctggtg
    22861 ttcactttgg tcaccaaact cgtcgctgga accctaagat ggctaagtac atcttcacag
    22921 aacgtaatgg tatccacgtt atcgaccttc aacaaactgt aaaattagct gaccaagctt
    22981 acgaatttgt tcgtgatgct gcagcaaacg atgcagttat cttgtttgtt ggtactaaga
    23041 aacaagcagc tgaagctgtt gctgaagaag ctaaacgtgc tggtcaatac ttcattaacc
    23101 accgttggtt aggtggaact cttactaact ggggaactat ccaaaaacgt attgctcgtt
    23161 tgaaagaaat caaacgtatg gaagaagaag gaacttttga acttcttcct aagaaagaag
    23221 ttgcattgtt aaacaaacaa cgtgcacgtc ttgaaaaatt cttgggtggt atcgaagata
    23281 tgccacgcat cccagatgta atgtatgttg ttgacccaca taaagagcaa atcgctgtta
    23341 aagaagctaa aaaacttggt attccagttg tagctatggt tgacactaac gctgatccag
    23401 atgatatcga tgttatcatc ccagcaaacg atgacgctat tcgcgccgtt aaattaatta
    23461 cttctaaatt agctgatgca gttattgaag gtcgtcaagg tgaagatgct gacgttgact
    23521 tcgctcaaga agcacaagca gattcaattg aagaaatcgt tgaagtagtt gaaggatcaa
    23581 acaacgacta atatttaatt tattgtaaat cctaaaacgg ggcagatgca aaccgctctg
    23641 tctcgttttt ttaatcatta tttgaaatta agaggagaac gaaaatggca gaaatcacag
    23701 ctaaacttgt aaaagaattg cgtgaaaaat caggtgcagg cgttatggac gctaaaaaag
    23761 cattagtaga aactgatggt gaccttgata aagcgattga attacttcgc gaaaaaggta
    23821 tggctaaagc agctaaaaaa gcagaccgtg ttgctgctga aggtttaaca ggtgtttatg
    23881 ttgatggtaa cgttgcagca gttattgaag ttaacgctga gactgacttt gttgctaaaa
    23941 acgaccaatt tgtaacatta gttaatgaaa ctgctaaagt tattgcagaa ggacgtcctt
    24001 caaacaatga agaagcactt gctttaacaa tgccttcagg tgaaacttta gaacaagcat
    24061 ttgtaactgc aactgctaca attggtgaaa aaatttcatt ccgtcgcttc gctttagttg
    24121 aaaagactga tgagcaacat tttggtgcat accaacataa tggtggacgt atcggagtaa
    24181 ttactgttgt tgaaggtggc gacgatgcac ttgctaaaca agtatcaatg cacgttgcag
    24241 ctatgaagcc aactgttctt tcatatactg aacttgatgc acaatttgtt catgatgaat
    24301 tagcacagtt gaaccacaaa attgagcaag acaacgagag tcgtgctatg gttaacaaac
    24361 cagctcttcc attccttaaa tatggatcaa aagctcaatt gactgatgaa gttattgccc
    24421 aagcagaaga agacatcaaa gcagaattag ctgcagaagg taaaccagaa aaaatctggg
    24481 ataaaatcgt tccaggtaaa atggatcgtt tcatgcttga caacactaaa gttgaccaag
    24541 agtacacact ccttgctcaa gtttacatca tggatgatag caaaacagtt gaagcttatc
    24601 ttgagtcagt aaatgctaaa gcagttgctt tcgtacgttt tgaagttgga gaaggtattg
    24661 agaaagcttc taacgacttt gaagcagaag ttgcagctac aatggcagca gcacttgaaa
    24721 aataattaac actcgcttta tattataatt tgacagcaaa atgttgtcag taaatatatt
    24781 ttaaaataag acacttggct taagtcaagt gtcttatttt gctatcgaat tggtggattg
    24841 gcaaatatta aaaaggagag acagtcatct caccttaact tttctagaat aggttgaagc
    24901 cgataatgga taatgttccc atgatgcatc cagctagtat aacacctagc ataacagcaa
    24961 taacactatg tttaaattcc caatctagga ggctagctgc aagcgtgcca gtccaggctc
    25021 ctgttccggg taagggaatg cctacaaaca atagcaaggc aataaacaat cctttttctc
    25081 cagctacttt ttcaagtttt tgaccaccgc tatgtccttt tttaaggcac catgtgaaaa
    25141 acttaccagt ataaggttta tctgctcccc attctagaac tttgcgagca aagaagaaga
    25201 tgataggtac aggtagcatg tttccaacaa caccgatagc tagcgcttcc cacaaaggta
    25261 ttccattagc gattgcaaat ggcacagctc ctcgtagttc cactaaggga atcattgaaa
    25321 tcaaaaatgt aataatgtaa ttcattaaat aaaacctttc tatggtatta ttccatttta
    25381 tcttatcttt ctagtaatga caactaaaat ccgcggtcat tctagtttta ctgtccttag
    25441 atagatttca tttccgtatc ttttaaaaat ttgatataat aatagtcagt tatagtcaaa
    25501 gaggaaggag tgaatttgtg gccataaaaa atacttccga taatattgaa gaatatataa
    25561 aatcattatt agaacaatca ggaattgctg aaataaagcg ttccaattta gctgatactt
    25621 ttcaagttgt tcctagtcag attaattatg tcattaagac acggtttact gaaagccgtg
    25681 gttatgtggt tgagagtaaa cgaggtggtg gaggttacat ccgcattgct aaggttcatt
    25741 tttctgatca acaccaatta tttggaaata tgttatcaac gattggcgag cgtatcagtg
    25801 aacaagtttt tgatgatttg attcaattat tattcgatga agagataatt acagaacgtg
    25861 aaggaaattt gattctggcg acatcaggtg atgacgtctt aggagagcaa gcctctgtta
    25921 ttagagctcg tatgctccgt aaattattac aacgattaga tagaaaagga ttccatttat
    25981 gagtcattat tccataaaat tacaagaagt attcagattg gcgcaatttc aagcagcacg
    26041 ctatgaaagt cactatttag aaagctggca tcttctttta gcaatggttt tagttcatga
    26101 ttcagtagct ggtttgacat ttgctgaata tgagtctgaa gttgcaattg aagagtacga
    26161 agcagcaaca attttagctt taggtagagc accgaaagaa gagattacca attatcaatt
    26221 tttagagcaa tcaccagctt tgaaaaagat cttaaagtta gcagaaaata ttagtatcgt
    26281 agtaggagct gaagatgtgg gtactgaaca tgtcttgctt gctatgttgg ttaataaaga
    26341 tttattagcc acacgtatct tagagttagt tggctttaga ggtcaagacg atggtgaatc
    26401 agttcgtatg gttgatttac gtaaggctct agaacgtcat gccggtttta caaaagatga
    26461 tatcaaagca atttatgaat tacgtaatcc taaaaaagca aagtcgggtg cttctttttc
    26521 tgatatgatg aaaccaccga gtactgctgg tgatttagct gatttcacac gtgatttatc
    26581 acagatggcg gttgatgggg aaattgaacc tgttattggg cgtgataaag aaatttcacg
    26641 tatggtccaa gtcttaagtc gtaaaacaaa gaataatcct gtcttagttg gagatgcggg
    26701 tgtcggtaaa acagctttgg cgtacggtct agcacaacgt attgctaatg gaaatattcc
    26761 ttatgagctt agagatatgc gtgttctaga gttagatatg atgagcgttg ttgctggaac
    26821 tcgttttcgt ggtgattttg aggaacgtat gaatcaaatc attgcagata ttgaagaaga
    26881 cggtcatatt attcttttca ttgatgaact gcatactatt atgggttctg gctctggtat
    26941 tgatagcaca cttgatgctg ctaatatttt aaaaccagct ttggcccgag gcactttacg
    27001 cactgttgga gcaacaactc aagaggaata tcaaaagcat attgaaaaag atgctgcttt
    27061 atctcgtcgc tttgcaaaag ttttagtaga agaaccaaat cttgaagacg catatgaaat
    27121 tctacttgga ttgaagccag cgtatgaagc ctttcataat gttactattt cagatgaagc
    27181 agttatgact gctgtaaaag tggctcatcg ttatttgaca agtaagaatt taccagactc
    27241 agctatagac ttacttgatg aagcttctgc cactgtgcaa atgatgatta agaaaaatgc
    27301 tccttcgctt ttaacagaag ttgaccaagc tattttagat gatgatatga aatctgcttc
    27361 taaagctcta aaagcaagtt ataagggtaa aaagcgtaag cctattgccg taacagaaga
    27421 tcatattatg gcaactctca gtcgtctatc tggtattcct gttgaaaaat tgacacaagc
    27481 tgatagtaaa aaatacctca atttagaaaa agaattgcat aaacgtgtta tcggtcaaga
    27541 tgatgcagtt actgccatta gtcgtgctat ccgtcgcaat caatctggca ttcgtactgg
    27601 taagcgtcct attggttcct ttatgttctt gggaccaact ggtgttggta agacagagtt
    27661 agcaaaagcc ttggcagaag tgttgtttga tgatgaatca gccctaattc gttttgatat
    27721 gtcggaatat atggaaaaat tcgcagcttc tcacctgaat ggggcacctc caggttatgt
    27781 tggttatgat gaaggaggag agttgactga aaaagtacgc aataaaccat attcagtttt
    27841 actttttgat gaagttgaaa aagctcatcc agatattttt aatgtattat tacaagtgct
    27901 tgatgatggc gttttaacag atagtcgagg acgaaaagtt gatttttcaa atactataat
    27961 cattatgact tctaatttag gagcaactgc attacgtgac gataaaacag ttggttttgg
    28021 agctaaggat atcagccatg attatactgc tatgcaaaaa cgtattatgg aggagcttaa
    28081 aaaagcttat cgaccagagt ttatcaatcg tattgatgag aaagttgtat tccatagtct
    28141 cagtcaagat aatatgcgtg aagttgtgaa aattatggtt aaacctttaa ttttagcgct
    28201 taaagataaa ggtatggatc tgaaattcca gccttcagcc ttgaaacact tggcagagga
    28261 tggctatgat atagaaatgg gtgctcgtcc actgcgtaga actattcaaa cgcaagtaga
    28321 ggatcattta tcagaactac tattggcaaa tcaagtgaaa gaggggcaag taattaagat
    28381 tggcgtatcc aaaggcaaat tgaaatttga tattgcaaaa tcataaaaca ttccagtacc
    28441 gatgggtact ggaattttaa tataaaaaga aaacgtccaa aatattttgg acattttctt
    28501 atagatttat gaaaagtaaa aggatctata tctaacaatt gcacttgtag atataggtac
    28561 gactgaaagt cgttgaaaaa ttttaggata aatatattat aatatcctaa tcttttgcta
    28621 acttaaaata aatctaaaga ttaccttaag atttcaatcg cttttgtaac cacacaatgt
    28681 catgccattg ttcaaattta aatccgattt ttgggaaatg tgcgacttga ttaaatcctc
    28741 tttttttgtg gaatgcaatg cttatatcat ttggtaaact gatacaagcg agtagattaa
    28801 catacccttg gttaactaat tctttctcca atgtatcata tagttggcta ccaatttttt
    28861 tgctacgtgc atctttatca aggtaaatag aaagctcgca agtccagtca taggcagctc
    28921 tagcgtagta agttgaagca tatgcataac ctaatatttt cccattttct tcagcaacta
    28981 aatatgggaa agatttgctg atatttttga tacgattagt gaattccgat atactaggaa
    29041 tatcgtattc aaacgttatt gtagtgtcag caacgtaggg agcatagata gcaagtaatt
    29101 ctttagcatc tgagggtagg acaggtctta taatcataga tatcacctaa ttatagtaaa
    29161 tcaagttttt gcaattcgga atcaatagtg tcaaggacgt gttgaagatc ctctggattt
    29221 ttgacgaaat caagtttgtt accatcgatc cgaattttag gtgaaacgtc gtaattttca
    29281 taccattctg gatattcaga atggacttgt ttgtagtagt cgtataactc tgggttagaa
    29341 tcaacttgct caaagctgcg tcctcgtttg tcaatacgtt ctaacatttt atcaaatgaa
    29401 acatcaatgt agactagcaa atcaggtctc ttcttaggca taccttctaa ttcttccaac
    29461 atatttgcaa gaagttcttt atagatatct agctctgttt tagtcacatt tccattctta
    29521 taatttagag tgaggaatag ttcatcttca aaaatagaac gatccaaaac attgttgtta
    29581 gccttatagg cttcttttat agattggaat cgtttattta aaaataatat ttgtagtaaa
    29641 aaagcatatt tttggggatc ttgatagtaa agatctaaaa ctgggttatt atcaaccgcc
    29701 tcatagaaaa catctgtccc taggtgttgt cctagagcag ctgcaaggga gcttttacct
    29761 gctccaatgg ttccagctaa aacaatcaac ataattctcc ttaattctgt tatgctaaaa
    29821 taatttgtta tacttttgag ttttcattac acaatgattt atgctaaatt tgtacagaga
    29881 taggaaaaaa cagtcaaagg actgtttttg atttagcgta aacctgcaaa aagttcttga
    29941 acttctgcta gtgtttctgc acgtgaaaca gctcctctaa tttttgctgc gcctgatttt
    30001 ccacgcagat aatgaggtgc taaccctcga aattcacgaa cggcaatagt ttcacctttt
    30061 aaattaacta gtcgagtgag atgatcctcg gcaacatcta acatcttctc aaatggtagg
    30121 tcaggaagga tttcgcctgt ttcaaagaaa tgattaattt gtgtaaagat gtaaggattg
    30181 ctacgagcac cacgtccaac cattatagcg tcagctccaa tctcttcaat cataaatttg
    30241 gcatcgtgaa cagtacgaat gtcaccatta gcaataaaag gaatactagt aacagcttta
    30301 gcgaccttgc caagtgtttc atggtcacac gtgccagtat acatttgttc acgcgttcga
    30361 ccgtgcatag caagtgcaga tacaccagca gattcggcag cgagtgcatt ttctatagca
    30421 ttagatgagt cagaccatcc tgtacgcatt tttactgtta atggaatgtc aagtacagac
    30481 gttacctctt taacaatatg ataaattttt tcagggtcac gtaaccattt agctccagct
    30541 tcgtttttaa caactttgtt aactggacat cccatattga tgtctacgat atctgctttc
    30601 gtattacttt ggataaagtc agctgcacgt tttaagcctt ctgcatcgcc accaaatagt
    30661 tgaattgaca taggatgttc attttcatcg atatggagca tatgtaaggt cttttcgttg
    30721 ttataaagta accctttctc agaaatcatt tccataacaa cgagaccagc accaaattct
    30781 ttagcgatag tacgaaaagc agaattggta atacctgcca tcggtgctag tacggtacga
    30841 tgaggaattt caaccttccc aatcataaat gaactattta attgagtcat taattatctt
    30901 ctccaaatct tcttctgtga aatagtaagt ctggttgcaa aattgacaag taatttcaac
    30961 acctttatct tcatccttca tggcttgcaa ttctttagtt cctaaagtca atagagcggc
    31021 ttcaaaacgt tcttttgaac agtcacagta gaaggctaaa gagtcttccg ataagcgctt
    31081 gtagtcgtct tctccgtaaa tagctgaaag gagagattct atatgatttt cagactctag
    31141 cagacttgaa atagaaggca tctcctgaat gcgtttttca tagcgagaaa tttcttcgtc
    31201 agaagcacct gggagcacct gaagcatgaa tccacctgca actttaacct tatcctcgtc
    31261 atctaagagg acatttaaac cgacggcaga aggtgtttgc tcactttcgg ttaaataata
    31321 agcaaaatct tctccaattt ctcctgtaat tagtggagtt gttgaagtgt aaggttgccc
    31381 agttgcgtaa tcggtaataa cgacaaagtg tccattaccc atgaaaggtc caactaagac
    31441 ctctccagta gctgttttct tgatatcaac acccgtattt tggatgtaac ctttaacatt
    31501 tcctttagta tcagctacag aaataatatg accaaaagag ctatctccaa taaccttgac
    31561 agttacttta ctatttcctt tttgatttgc tgctaaaatt tgatttgcaa ttaatgttct
    31621 cccaagagct acagtagaac ttgaaagtgt ctgatgtttt tcttgagctg ttcttacagt
    31681 ttctgtacaa tcaaggacat aagcccggaa agaaccggaa gttgaaattg attttataat
    31741 tttatccata agtcatatta tagcataaaa ggcttatttt gcattggaga tagtcaggaa
    31801 aatatagatg aaaacggtat aaattaatga tataaaaacg atactgtgtt ttacagtatc
    31861 gtttttattt agtggatatg atagtaagtt aagactcatc aactttttct tgggtagatt
    31921 taccttcgtt gagtaaggct tcaactattt ttttatcacg aattttgaca gaatcattga
    31981 ttgtttcagc cgatttaata acagcagatt ccaattgggc acgttcctta cgtcctttgt
    32041 ctatggcagc gataatacca ttattttgag ccactaagct ttcagctaat gcagtgacag
    32101 atttaataga aacagtgggg ctttgtgcgg tcttctctaa catcggaatc gcttctttac
    32161 tagtttcagc cagcatctgc aatgctgcat tattagcgtt gacaatagca tcagcagtga
    32221 caccggattt gacagattgt tgcatcatgc ctaactgagc gattgagagt ttcattgttg
    32281 gaatggtatt tcgacgtaac atgccaagtt tctgacgcat atctgacgat actttgacca
    32341 agtttcgcat ctgtggtgtt gttgcccatg caacgtagag acggctgaca tattcaggat
    32401 gttgctgttc gagggtattg ataacttcag tcattcgagc taattggtta cttttaattt
    32461 gatactcgga cgtttggcta tctaatgcta gaatttcttg ttgtaagtgg cttgcacgat
    32521 tagcagcctc ggcttgactc gattcaataa aagcaataac tccaaccaaa ttttcaatag
    32581 atttagtatt atcttcaatg agcatttcag cagagacgat atttcttgcc aaagtatctt
    32641 cttgtttgac aacattcgct gccatcatat ccattttttg ctcgatgttt tgtgagtcaa
    32701 aataaaattc ctgtagcgag gtcttgcttt gtttgaataa tttttgaatc aagtttggtt
    32761 ttttctctaa ttctgccgga gtagcatctt tatatttggc aataaatcca tttagttcgc
    32821 gattagcatt ttttagtaaa tcatcaactt gaggaatttg aatttttttc tgctcagaca
    32881 agatatgatt aacagtggta ttaacgcctt ctactgcgga ttgtccaaaa tcaaggagcg
    32941 cattttgatc gccgacaaaa gtatctacca aagctggtgt tttttcagag atagcagact
    33001 tttgtgctgg tgttagtttt tcaaaaaagg caatttgccc agtttggctt gttgtctggt
    33061 tggaaataat ttctgttgtt ttatctgttt tagtgatagc attgtctgca atttggtcaa
    33121 tatcaaaatt aaaggtatcg ctcatttact gttcctttcg tcttgcatca ttcttaggct
    33181 aacgtcaaaa tctttcatat cactttcatt caattggcgc agcgtttcat ctaaatctaa
    33241 attaaatttt ttaattgctt gtaatgcttg ttctaggcgt tccttagcat tgttaaaatt
    33301 tttaggagat tgtttgattc tgatgtagcc agccagaaca tctgtaaaac gtttcatatt
    33361 ggcttgatga attgctgcta attcagcttt gttatctgcc tcttcaatct tttttaagat
    33421 agcaaagtgg tcatcttgaa tattgtgata gaacggtttt aattccggag caatgcgtat
    33481 catttcatct tcctgatcag aaataggttc tagttctgga gaggcatgta gctttattag
    33541 ttgttcgtta atctcattac taaatgtatt tgcttttttg gtgatacgtt tatagatatt
    33601 aggctcaatg gctttttgta gttggtttgc ttcattacga attaggtcca attgaggtaa
    33661 tatttgatgt gctaatttga tatattcctt agaacgattg tcttttttta aaatttccaa
    33721 gtgtctaatg tcttggtcag tctctttaat atcggatttt aaagcttcaa tacgttggtt
    33781 tgatgtaaaa cgtttaagac gctttttatc ataaacgtaa taggcacaat aaccaccacc
    33841 aataattaac agagtagcaa tcacctcact ccaaaagtct gaaatcaggg aagcaagaac
    33901 aacaatacca agtaatgtta ttagattatg agaatttttg gaatagcgtt ttttaaccat
    33961 aataatattt aacctaactt tctgtagttg ttaatatttt atcttaaatg aactagtgat
    34021 gacaagggat aactttattt taaccaaaaa ttccttaagc ttagcctaag gaactctttt
    34081 tagttataat tctgggaaca gtttagccaa aattcgaggt gtaatagatt gaccaggccc
    34141 ctcttgacag taggtgtgca tatacatcaa tgggttaaag tttagttcaa tgcaagtagc
    34201 atttttctta tcttttgagt atgcttgagt ggcattagga ataattaaat caactccaca
    34261 aacccaagcc cccatagcct ctgccatctc agctgctagt tgtttatatg taggatccat
    34321 tgtatttgtt acatctatag agtcaccgcc agtggaaata ttagaattac ggcgaagttc
    34381 tatttttgtt ccttctggag ggatactgtt aacagtgtat ccttgttgtt ccaacattaa
    34441 ctgttctact tctccgagct caataacctc tagaggagag cgatgatcat agcctctgag
    34501 agggttttgg tttttaagtt taactagctg actaatagta tgaataccat ctccaacgac
    34561 attagctgct accctaagca atacagcaat acagtccccc tcaaggacga agaaacggta
    34621 ttcggtaccc tcaatatatt cttccacaag aatagcacta tcttcggtaa aagcgatgtc
    34681 aattgctttt tcataagaag ctaagttagc tgatgtttta aaaatagaaa tgcctaaacc
    34741 aaagtttgta gattttggct taacgacgat tggtttatct tggatttgtg aaaaatagtt
    34801 aagtgcctct ttacggtcag taaattcatc tccaaaagga gttgggaaat gcttttcatc
    34861 taggattttt tttgtaacaa ctttgttagc catagcgagc ggtacaatat agttatcttt
    34921 tgaggtcata ttaccatttt taacgtactc aatatgacta ttgtgccata attttaggaa
    34981 ttgatcttgt tcatccagca cttcgaagtt gactcctttt tggataacgt caaatagtag
    35041 caattgggta gaaagttcca tcgtttcata tccctttaag gcgtagggag cttcccaagc
    35101 ataatcgtga taaatttgtc cttgtctttg tccaaatgtt tctaaggaaa gtccttcaat
    35161 catttctaaa agttgaccag ctaccgttaa ttcaggtgac tgaatttgcc tttttacaga
    35221 ttccaatagg tcttgatagt aaggtgataa gttaaaatgt tggataacag attgcatagc
    35281 atccactaag tcagaaactg gtgcttgatt aggtaatttt tctaatggat gactaagtgc
    35341 tataaggtca tttaatctat tggcttcctt gatatcttgg tcaatatgac tactagaatc
    35401 tatccacaaa agcgccaata agaaaaggtg aacagtatca acagtttcct gcgttatacc
    35461 gattgggcta aatgggttga gatcaaaagt gcgaaactct aaatatgtaa ttcctttttc
    35521 taaataatta cgacaggcct tactaccacg gagacgaaca ggtgaataaa attctttctc
    35581 agcaatcaat tgcccacttt ttacagcatt ttctagatcg ttgacataat ccttcaagct
    35641 agtataagaa atacgaatat ctttatgatt cacataacct aagtgactat ttctaagtga
    35701 ccggacaggg ttattcagtt tctgatctaa aaagtcttct tcagctactg ggctcgctcc
    35761 gtatagataa gttagtaacc aacggtaacg taaaaaattt tgagacaatt tcatgtaaag
    35821 ctggttctga aaatcaatag cattatctgc ttgactcaat tcaaagagag aagttaataa
    35881 ctcttgacct aaaccgagat tatagtgaat tccagagatg gattgtatta gttttccata
    35941 agtcttctcc aaatatttgc gataatcata ttcaaaagca tcctctaatt gggcaatctg
    36001 tatgtcttct tccctcactt tgggtggcat agaaagcggc cataagtatt catcgtggtt
    36061 gatagagcgt ccagcaacat cacttatagc tttcaaaaat cgaatggctt cttggctatc
    36121 ctttgcaata ggtgtaatga gttctaattg aggttcacta taatccgttt ggatataagg
    36181 atgatagtta cgactcccta atgttttggg atggggtgtt tgagcaaccc tttgagtggg
    36241 ttggtgaata cgaaggcttt ctctctctaa gccaaatgta gcttgtagaa tcggtagatg
    36301 agagtggctt ctttgtaaca gtcgatcgat aatcacgaca acattcctcc tagtaacaga
    36361 tgatactagt ttatcaaaaa aataataaaa actaagtttt acggcacaga aaactaaaat
    36421 aatcttatac cggtgtcata aaaaataaaa
//
LOCUS       DATRMA010000014        33531 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.014, whole genome shotgun sequence.
ACCESSION   DATRMA010000014 DATRMA010000000
VERSION     DATRMA010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 33531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33531
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.014"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            complement(123..605)
                     /locus_tag="VBC28_000642"
     CDS             complement(123..605)
                     /locus_tag="VBC28_000642"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002885585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HEO6814643.1"
                     /translation="MTKEVVVESFELDHTIVKAPYVRLISEEVGPVGDIITNFDIRLI
                     QPNENAIDTAGLHTIEHLLAKLIRQRINGLIDCSPFGCRTGFHMIMWGKQDATEIAKV
                     IKSSLEAIAGGVTWEDVPGTTIESCGNYKDHSLHSAQEWAKLILSQGISDNAFERHIV
                     "
     gene            794..2401
                     /locus_tag="VBC28_000643"
     CDS             794..2401
                     /locus_tag="VBC28_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEO6814644.1"
                     /translation="MFNIILAMVCALIGLIIGYVAISMKMKSSKEAAELTLLNAEQDA
                     VDLRGKAEIEAEHIRKAAERESKAHQKELLLEAKEEARKYREEIEKEFKSDRQELKQM
                     EARLTDRASSLDRKDENLSNKEKMLDSKEQSLTDKSRHINEREQEIATLETKKVEELS
                     RIAELSQEEAKDIILADTEKDLAHDIATRIKEAEREVKDRSNKIAKDLLAQAMQRLAG
                     EYVTEQTITTVHLPDDNMKGRIIGREGRNIRTLESLTGIDVIIDDTPEVVVLSGFDPI
                     RREIARMTLESLIQDGRIHPARIEELVEKNRLEMDQRIREYGEAAAYEIGAPNLHPDL
                     IKIMGRLQFRTSYGQNVLRHSVEVGKLAGILAGELGENVDLARRAGFLHDMGKAIDRE
                     VEGSHVEIGMEFARKYKEHPIVVNTIASHHGDVEPDSVIAVIVAAADALSSARPGARN
                     ESMENYIKRLRDLEEIANGFEGVQNAFALQAGREIRIMVQPGKVSDDQVVIMSHKVRE
                     KIEQNLDYPGNIKVTVIREMRAVDFAK"
     gene            2530..3426
                     /locus_tag="VBC28_000644"
     CDS             2530..3426
                     /locus_tag="VBC28_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6814645.1"
                     /translation="MAKDIIKVDHIFKSIGQKTILEDISFSIASNQCVALIGPNGAGK
                     TTLMSTLLGDISISSGSLTIFNLPAHHNRLKYKVAILPQENVLPSKFTVRELIDFQRC
                     LFPEVLPMSLILDYLQWSDTHLQQFTETLSGGQKRLLAFVLTLVGKPQLLFLDEPTSG
                     MDTSTRQRFWELIATLKKEGVTIVYSSHYIEEVEHTADRILVLHKGKLLRDTTPFAMK
                     QEKTEKLFTVPLSYQKLLPTYLITECEAKSDSITFVTGEAEAVWKILADNGCPIEAIE
                     MTNRTLLNRIFETTKEVKHENL"
     gene            3413..4153
                     /locus_tag="VBC28_000645"
     CDS             3413..4153
                     /locus_tag="VBC28_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6814646.1"
                     /translation="MRIFKALLKMEFLLTKRQLANLIMAIGMPVAFFLFFSGFMGEGL
                     TKAIEAIYVRNYMITMAGFSSLSFAFFTFPFSMKDDQLSNRMQLLRHSPVPMWQYYLA
                     KIIRILFYYCLAITVVFLTGHILRQVSMPIEQWMQSFLLLLGGATCFIPFGLLVSYFK
                     NTELMSMVANICYMSLAVLGGMWMPITMFPKWLQALSKLTPTYHLTQVILSPFANSFA
                     GFSLIILIGYGIIMLVIAYLLSQKRHSI"
     gene            4150..5235
                     /locus_tag="VBC28_000646"
     CDS             4150..5235
                     /locus_tag="VBC28_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000791351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEO6814647.1"
                     /translation="MKNKKNNYDLMYFIPLVFLIYPIGGILYYHYPFWTLFFTLAFVG
                     AYLYSVIIRGESKYHMIAWSTMLTYIFYMTIFINSGFIWYIYFLSNLLVYRFRDKLKS
                     FRFISFACTLATVVFLCFFKASDFGDRIMFLIVPIFCIGYMWIAIENRNSEEQREKIA
                     EQNQYINILSAENERNRIGRDLHDSLGHTFAMMTLKTELALKLLEKRNYDKVQKELSE
                     LNHISHQSMSEVRQIVSNLKYRTVVEEIDELYRLFQLSNIKLTVVNKLETSQLSPVTQ
                     STITMILKELSNNIVKHAEADSVELSLVRQGATINIEMIDNGCGFTNLDGDELHSIQE
                     RLTIVEGTLTILSRSKPTHIQVVLKEV"
     gene            5237..5827
                     /locus_tag="VBC28_000647"
     CDS             5237..5827
                     /locus_tag="VBC28_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO6814648.1"
                     /translation="MKLLVAEDQSMLRDAMCQLLLMEESVSTIDQAGNGGEAIAILSN
                     KAIDVAILDVEMPILSGLDVLEWVRKYQNVKVIIVTTFKRSGYFQRAIRSNVDAYVLK
                     DRSVADLMKTIQKVLSGGKEYSPELMENVISNPLSEQEIKILSLIAQGKTSKEIACTL
                     FLSNGTVRNYTSNIFDKLGVNNRLEALKIAREKDWL"
     gene            5877..6581
                     /locus_tag="VBC28_000648"
     CDS             5877..6581
                     /locus_tag="VBC28_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001168921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HEO6814649.1"
                     /translation="MQKFKERFDTLSDAILAIAMTILVLEIKTPATMGDIGDFTRNIG
                     LFIVSFVVVFNFWYERAQNSLDAQKTNDEIIALDIIEHLGICLIPLFTKFMISFENHN
                     FAVMAYGLLTLLVGLTSDIIRIRLASYDLVTIPSELKERVIKVMTTFAIRSVVVRFII
                     IILAYFLPEVGIFAYLVIPLFMFWRRSRAGKYYAKKGIEAPSYIKLFIQIGERAPEDF
                     TNSSSGIGGVKRLYKK"
     gene            6748..7377
                     /gene="gmk"
                     /locus_tag="VBC28_000649"
     CDS             6748..7377
                     /gene="gmk"
                     /locus_tag="VBC28_000649"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEO6814650.1"
                     /translation="MSERGLLIVFSGPSGVGKGTVRQEIFSTPDHKFDYSVSMTTRPQ
                     RPGEVDGVDYFFRTREEFEALIKEGQMLEYAEYVGNYYGTPLSYVNETLDKGIDVFLE
                     IEVQGALQVKSKVPDGVFIFLTPPDLEELEERLVGRGTDSPEVIAQRIERAKEEIALM
                     REYDYAVVNDQVSLAAERVKRVIEAEHYRVDRVIGRYTNMVKETDKKLS"
     gene            7400..7714
                     /locus_tag="VBC28_000650"
     CDS             7400..7714
                     /locus_tag="VBC28_000650"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEO6814651.1"
                     /translation="MMLKPSIDTLLDKVPSKYSLVILQAKRAHELEAGEKATQDFKSV
                     KSTLRALEEIESGNVVIHPDPSAKRASVRARIEAERLAKEEEERKIKEQIAKEKEDGE
                     KI"
     gene            7788..10178
                     /locus_tag="VBC28_000651"
     CDS             7788..10178
                     /locus_tag="VBC28_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000101529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEO6814652.1"
                     /translation="MARKLAQVIVDIPLMQTDKPFSYAIPKDLEDLVQVGVRVHVPFG
                     RGNRLLQGFVVGFRDDDELETKDIAEVLDFEPVLNQEQLDLADQMRHTVFSYKISILK
                     SMLPSLLNSQYDKLLLATDTLPSEDREDLFGHKTEIVFSSLSSQDAKKAGRLIQKGFI
                     EVQYLAKDKKTIKTEKIYKINRTLLEKSQIAARAKKRLELKEFLLENPQPGRLTALNK
                     QFSSPVVNFFREEGIIEVIEKEASRSDNYFKGILKTDFLDLNQEQAKVVKIVVDQIGK
                     EQNKPFLLEGITGSGKTEVYLHIIDNVLKLGKTAIVLVPEISLTPQMTNRFISRFGKQ
                     VAIMHSGLSEGEKFDEWRKIKSGQAKVVVGARSAIFAPLENIGAIIIDEEHESTYKQE
                     SNPRYHARDVALLRAEYYKAVLLMGSATPSIESRARASRDVYKFLELKHRANPKARIP
                     QVEIIDFRNFIGQQEVSNFTSYLLDKIRDRLDKKEQVVLMLNRRGYSSFIMCRDCGYV
                     DQCPNCDISLTLHMATKTMNCHYCGFEKPIPRTCPNCNSKSISYYGTGTQKAYEELLK
                     VIPDAKILRMDVDTTRQKGGHESILKRFGNHEADILLGTQMIAKGLDFPNVTLVGVLN
                     ADTSLNLPDFRSSERTFQLLTQVAGRAGRAEKEGEVVIQTYNPNHYAIQLAQKQDFEA
                     FYQYEMNIRRQLGYPPYYFTVGLTLSHKDEEWLIRKSYEVLSLLKQGFSDKVKLLGPT
                     PKPIARTHNLYHYQIIIKYRFEDNLELVLNRLLDMTQDKENRDLRLAIDHEPQNMM"
     gene            10225..11160
                     /locus_tag="VBC28_000652"
     CDS             10225..11160
                     /locus_tag="VBC28_000652"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEO6814653.1"
                     /translation="MTKLLFMGTPDFSATVLKGILADGKYDVLAVVTQPDRAVGRKKE
                     IKMTPVKEVALENNIPVYQPEKLSGSPELEQLMTLGADGIVTAAFGQFLPTKLLESVG
                     FAINVHASLLPKYRGGAPIHYAIINGEKEAGVTIMEMVAKMDAGDMVSKASVEITDED
                     NVGTMFDRLAVVGRDLLLDTLPGYLSGDIKPIPQNEEEVSFSPNISPDEERIDWNKSS
                     RDIFNHVRGMYPWPVAHTLLEGNRFKLYEVTMSEGKGSPGQVIAKTKNSLTVATGDGA
                     IELKSVQPAGKPRMDIKDFLNGVGRNLEIGDKFGE"
     gene            11150..12472
                     /gene="rsmB"
                     /locus_tag="VBC28_000653"
     CDS             11150..12472
                     /gene="rsmB"
                     /locus_tag="VBC28_000653"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEO6814654.1"
                     /translation="MANDWKKSARGLALMTLEEVFDKGAYSNIALNKSLKKSRLSDKD
                     RALVTEIVYGTVARKITLEWYLSHFIVDRDKLELWVYHLLLLSLYQLLYLDNIPDHAI
                     VNDAVTIAKNRGNKKGAEKLINAVLRRVSSETLPEIASIKRQNKRYSVAYSMPVWLVK
                     KLIDQYGETRALAIMESLFERNKASLRVTDLSQKQTIKETLNVRDSHIAETALVADSG
                     NFASTSFFQDGLITIQDESSQLVAPTLKVSGNDQVLDACSAPGGKTSHIASYLTTGAV
                     TALDLYDHKLELVMENAKRLGLSDKIKTKKLDASKAHEYFLEDTFDKILVDAPCSGIG
                     LIRRKPDIKYNKANQDFEALQEIQLSILSSVCQTLRKGGIITYSTCTIFEEENFQVIE
                     KFLENHPNFEQVELSHTQEDIVKRGCISISPEQYHTDGFFIGQVKRIL"
     gene            12510..13247
                     /locus_tag="VBC28_000654"
     CDS             12510..13247
                     /locus_tag="VBC28_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEO6814655.1"
                     /translation="MEISLLTDIGQRRSNNQDFINQFENKAGVPLIILADGMGGHRAG
                     NIASEMTVTDLGSDWAETDFSELSEIRDWMLVSIETENRKIYELGQSDDYKGMGTTIE
                     AVAIVGDNIIFAHVGDSRIGIVRQGEYHLLTSDHSLVNELVKAGQLTEEEAASHPQKN
                     IITQSIGQANPVEPDLGVHLLEEGDYLVVNSDGLTNMLSNADIATVLTQEKTLDDKNQ
                     DLITLANHRGGLDNITVALVYVESEAV"
     gene            13247..15202
                     /gene="pknB"
                     /locus_tag="VBC28_000655"
     CDS             13247..15202
                     /gene="pknB"
                     /locus_tag="VBC28_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000614563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HEO6814656.1"
                     /translation="MIQIGKLFAGRYRILKSIGRGGMADVYLARDLILDNEEVAIKVL
                     RTNYQTDQIAVARFQREARAMAELTHPNIVAIRDIGEEDGQQFLVMEYVDGFDLKKYI
                     QDNAPLSNNEVVRIMNEVLSAMSLAHQKGIVHRDLKPQNILLTKKGTVKVTDFGIAVA
                     FAETSLTQTNSMLGSVHYLSPEQARGSKATVQSDIYAMGIMLFEMLTGHIPYDGDSAV
                     TIALQHFQKPLPSILAENKSVPQALENIVIKATAKKLTDRYKTTYEMGRDLSTALSST
                     RHREPKLVFNDTESTKTLPKVTSTVSSLTTEQLLRNQKQAKTTEKITPDSASNDKTKS
                     KKKASHRLLGTIMKLFFALCVVGIIVFAYKILVSPTTIRVPDVSNKTVAQAKMTLENS
                     GLKVGAIRNIESDSVSEGLVVKTDPAAGRSRREGAKVNLYIATPNKSFTLGNYKEHNY
                     KDILKDLQGKGVKKSLIKVKRKINNDYTTGTILAQSLPEGTSFNPDGNKKLTLTVAVN
                     DPMIMPDVTGMTVGEVIETLTDLGLDADNLVFYQMQNGVYQTVVTPPSSSKIASQDPY
                     YGGEVGLRRGDKVKLYLLGSKTTNNSSSTPIDSSASSSTGTTTSDSVSSSTDASTSDS
                     SSTSTSSSTLPSDSTTNTGTANNPLTQ"
     gene            15363..16058
                     /locus_tag="VBC28_000656"
     CDS             15363..16058
                     /locus_tag="VBC28_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEO6814657.1"
                     /translation="MKKFQFFLLVEAVVLVMGLMKILSDDWTSFIFILALILLALRFY
                     NNDSRHNFLLTTSLLLLFLIFMLNPYIIAAVVFAVLYVLINHFSQVKKKNRYALIQFK
                     NHQLDVKTTRNQWLGTDQHESDFYAFEDINIIRISGTDTIDLTNVIVSGQDNVIIIQK
                     VFGDTKVLVPLDVAVKADISSVYGSVQYFDFEEYDLRNESIKLSQEEEYYLLKRVKLV
                     VNTIAGKVEVSRK"
     gene            16055..17074
                     /locus_tag="VBC28_000657"
     CDS             16055..17074
                     /locus_tag="VBC28_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEO6814658.1"
                     /translation="MKKHHYFLAFFYGSVIIFAICFVIIDSLGVNLVHLYQTSRLWLI
                     EQLIFSIFFLSLAVTILLLLTWFLLDDNSKRQINHNLRRILNNQSINVTDDGTEISTN
                     IQRLSKKMNLMTASLQSKENSRILKSQEIVKQERKRIARDLHDTVSQDLFAASMVLSG
                     IAQNVSQLDVDQVGSQLLAVEEMLQHAQNDLRILLLHLRPVELENKTLSEGFRMILKE
                     LTDKSDIEVVYHESILTLPKKIEDNIFRIGQEFISNTLKHSQASRLEVYLNQTENELQ
                     LKMIDNGIGFDMDSVYDLSYGLKNIEDRVEDLAGNLQLLSQPGKGVAMDIRLPLVNQS
                     EDKNG"
     gene            17067..17708
                     /locus_tag="VBC28_000658"
     CDS             17067..17708
                     /locus_tag="VBC28_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO6814659.1"
                     /translation="MDKIKIVLVDDHEMVRLGLKSFLNLQADVEVIGEASNGLEGIKK
                     ALELRPDVVVMDLVMPEMDGVEATLALLKDWPEAAILVLTSYLDNEKIYPVIEAGAKG
                     YMLKTSSAAEILNAIRKVSRGEQAIENEVDKKIKAHDKCPALHEGLTARERDILNLLA
                     KGYDNQRIADELFISLKTVKTHVSNILGKLNVADRTQAVVYAFQHHLVPQDDE"
     gene            17750..19150
                     /locus_tag="VBC28_000659"
     CDS             17750..19150
                     /locus_tag="VBC28_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional Cof-type HAD-IIB family
                     hydrolase/peptidylprolyl isomerase"
                     /protein_id="HEO6814660.1"
                     /translation="MDAKTKYKAKKIKAVFFDIDDTLRVKDTGYMPPSILKVFKALKD
                     KGIVVGIASGRARYGVPKEVQDLNADYCVKLNGAYVKDKDKNIIFHRPIPAEYVEQYK
                     KWADTVGIKYGLAGRHEAVLSDRDDLVNDAIDIVYSDLEVNPDFNKEHDIYQMWTFED
                     KGDSLHLPEPLAEHLRLIRWHDHSSDVVLKGTSKALGVSKVVEHLGLKPENILVFGDE
                     LNDLELFDYAGLAVAMGVSHPEAQKKADFITKKVEEDGILYALEELGLIEKELTFPQV
                     DIENTEGPVAVIKTNHGDMTVKLFPDHAPKTVANFIGLAKQGYYDGIIFHRIIPDFMI
                     QGGDPTGTGMGGESIYGESFEDEFSEELYNVRGALSMANAGPNTNGSQFFIVQNTKIP
                     YAKKELERGGWPTPIAELYAGQGGTPHLDRRHSVFGQLVDQSSFEVLDEIAAVETGSQ
                     DKPLEDVVILTIEVKE"
     gene            19152..19508
                     /locus_tag="VBC28_000660"
     CDS             19152..19508
                     /locus_tag="VBC28_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEO6814661.1"
                     /translation="MKIGDKLKGTVTGIRPYGAFVSLEDGRTGLIHISEIKTGYIDNI
                     YDVLSVGDEVYVQVIDVDEFTQKASLSLRTLEEERHHIQHRHRFSNNRLKIGFKPLEE
                     NLPSWVEEGLAYLNNK"
     gene            complement(19555..20331)
                     /locus_tag="VBC28_000661"
     CDS             complement(19555..20331)
                     /locus_tag="VBC28_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HEO6814662.1"
                     /translation="MTNEKGIVFNIQHFSIHDGPGIRTTVFLKGCPLRCPWCANPESQ
                     KMVPETMRDAITNESVIVGEEKSVDDIIEEVLKDIDFYEESGGGITLSGGEIFAQFEF
                     AKAILKRAKSLGIHTAIETTAYTRHEQFIDLIQYVDFIYTDLKHYNSLKHQEKTMVKN
                     ASIIKNIHYAFANGKTIVLRIPVIPNFNDSLEDAEEFACLFDRLDIRQVQLLPFHQFG
                     QNKYQLLNRQYEMEEIAALHPEDLLDYQAIFSKYNIHCYF"
     gene            20452..21207
                     /locus_tag="VBC28_000662"
     CDS             20452..21207
                     /locus_tag="VBC28_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEO6814663.1"
                     /translation="MNRLENIISLVSQYQKIDVNTLSELLQVSKVTIRKDLDKLEGKG
                     LLHREHGYAVLNSGDDLNVRLSFNHKTKKEIAALAANMVSDNDTILIESGSTCALLAE
                     NICQTKRNVTILTNSCFIANYLREYDSCQIVLLGGEYQSSSQVTVGPLLKKMISLFHV
                     SLAFVGTDGFDPKLGFMGRDLMRSEVAQEMANAADEVIILTDSSKFNQTALVEQLPLS
                     TVSQVITDKHPNSEIANLFQEAEITIQSLLQVP"
     gene            21326..22309
                     /locus_tag="VBC28_000663"
     CDS             21326..22309
                     /locus_tag="VBC28_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000764457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HEO6814664.1"
                     /translation="MKLERQKLLAKVAYLYYMEGKSQSEIANELGIYRTTISRMLAKA
                     REEGLVRIEISDFNPEIFQLESYFKSKYHLKDIEIVSSRKDSDTSEIEKDLAHVAAAM
                     IRKKIKENDKVGIAWGRTLSKVVEAMRPHPVSQVSFVPLAGGPSHINARYHVNTLVYE
                     MSRRFQGSCTFINATLVQENANLAKGILTSKYFEGLMDNWEKLDVAIVGVGGKPKSNE
                     QQWLDLLNQDDFQCLDEEAAVGEITCRFFNHSGDPVNQHLAKRTIGITLEQLQKVPNR
                     IAVAHGNYKAAALLAVLKKGYINHLVTDFSTALNILRLDKDTFVDTIYQKS"
     gene            22513..22836
                     /locus_tag="VBC28_000664"
     CDS             22513..22836
                     /locus_tag="VBC28_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEO6814665.1"
                     /translation="MEIIVADQIIMGLILNAGDAKQHIYQALKLAKEGNFAESKIEIE
                     LADSALLEAHNLQTQFLAQEAGGTRTDISALFIHSQDHLMTSITEINLIKEIIDLRQE
                     LHIQK"
     gene            22853..23173
                     /locus_tag="VBC28_000665"
     CDS             22853..23173
                     /locus_tag="VBC28_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEO6814666.1"
                     /translation="MIKIGLFCAAGFSTGMLVNNMKIAADKEGIEAHIEAYSQGKIAD
                     YAKDLDVALLGPQVSYTLDKSKSICDEYGVPIAVIPMADYGMLDGVKVLKLALSLLET
                     RKEA"
     gene            23175..24476
                     /locus_tag="VBC28_000666"
     CDS             23175..24476
                     /locus_tag="VBC28_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEO6814667.1"
                     /translation="MSKFDSQKIITPIMKFVNMRGIIALKDGMLAILPLTVVGSLFLI
                     LGQLPFKGLNQAIANVFGPEWTEPFMQVYSGTFAIMGLISCFAIAYAYAKNSSVEPLP
                     AGVLSLSSFFILMKSSYIPVKGEAIADAISKVWFGGQGIIGAIIIGLVVGAIYTWFIQ
                     HHIVIKMPEQVPQAIAKQFEAMIPAFVIFLLSMIVYLIAKVTTGGTFIEMIYDIIQVP
                     LQGLTGSLYGAIGIAFFISFLWWFGVHGQSVVNGIVTALLLSNLDANKSLLAANRLTL
                     DNGAHIVTQQFLDSFLILSGSGITFGLVIAMLFAAKSKQYKALGKVAAFPAIFNVNEP
                     IVFGFPIVMNPVMFLPFILVPVLAALIVYGAIAVGFMQPFSGVTLPWSTPAIISGFMV
                     GGWQGALVQIVILAISTAVYFPFFKIQDNITYKNECEMERG"
     gene            24657..27113
                     /locus_tag="VBC28_000667"
     CDS             24657..27113
                     /locus_tag="VBC28_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HEO6814668.1"
                     /translation="MTKQLLTQKYTNDVQQNSQKHFGYLTERMYSYRDKVLDKKPFID
                     AERAILVTEAYQKHQEKPNVLKRAYMLQNILEKMTIYIDDETMIVGNQASSDKDAPIF
                     PEYTLEFVVNELDLFEKRDGDVFYITEETKEQIRNIAPFWENNNLRARAGVMLPEEVQ
                     VYMETGFFGMEGKMNSGDAHLAVNYQKLLEEGLIGFEKKARKAKADLDLTKPESIDKY
                     HFYDSILITIEAVKTYAERFAILAKKQAKTANAKRRQELLDIASICERVPYYPAETFA
                     EAVQSVWFIQCILQIESNGHSLSYGRFDQYMYPYVKSDLEAGRETEDSIVERLTNLWI
                     KTITINKVRSQAHTFSSAGSPLYQNVTIGGQTRHKEDAVNPLSFLVLKSVAQTHLPQP
                     NLTVRYHANLDKSFMNEAIEVMKLGFGMPAFNNDEIIIPSFIKKGVSEEDAYDYSAIG
                     CVETAVPGKWGYRCTGMSYINFPKVLLITMNDGIDPASGKRFAPSYGHFTQMTSYKEL
                     KEAWDKTLRYLTRMSVIVENAIDISLEREVPDILCSALTDDCIGRGKHLKEGGAVYDY
                     ISGLQVGIANLSDSLAALKKLVFEEKRLTTLEVWQALQSDYAGPRGEEIRQMLINEAP
                     KYGNDDDYADSLVRECYDVYVEEIAKYPNTRYGRGPIGGIRYSGTSSISANVGQGRGT
                     LATPDGRHAGTPLAEGCSPSHNMDKKGPTSVLKSVSKLPTDEIVGGVLLNQKVNPQTL
                     AKEEDKQKLIALLRTFFNRLHGYHIQYNVVSRETLIDAQKHPEKHRDLIVRVAGYSAF
                     FNVLSKATQDDIIARTEHAL"
     gene            27123..27791
                     /locus_tag="VBC28_000668"
     CDS             27123..27791
                     /locus_tag="VBC28_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEO6814669.1"
                     /translation="MEFLLDTLNLEAIKKWHHILPLAGVTSNPTIAKKEGDIHFFQRI
                     RDVREIIGREASLHVQVVAKDYQGILDDAAKIRQETDDDIYIKVPVTPDGLAAIKTLK
                     AEGYNITATAIYTSMQGLLAISAGADYLAPYFNRMENLDIDATQVIKELAQAIERTGS
                     SSKILAASFKNASQVTKALSQGAQSITAGPDIFESVFAMPSIAKAVNDFADDWKASQH
                     SEHI"
     gene            27859..28947
                     /locus_tag="VBC28_000669"
     CDS             27859..28947
                     /locus_tag="VBC28_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydrogenase"
                     /protein_id="HEO6814670.1"
                     /translation="MKVFASPSRYIQGKDALFQSIEHIKSLGQTPLILCDDVVYNIVG
                     ERFLSYLQDDLLPHRVSFNGEASDNEINRVVAVAKEKNSDLIIGLGGGKTIDSAKAIA
                     DKVNLPVVIAPTVASTDAPTSALSVIYTDEGAFEKYIFYSKNPDLVLVDTQVIAQAPK
                     RLLASGIADGLATWVEARAVLQKNGIAMAGGRQTLAGVAIAQACERTLFNDSLQALAA
                     CDAKVVTKALENVIEANTLLSGLGFESAGLAAAHAIHNGFTALSGDIHHLTHGEKVAY
                     GTLTQLFLENRPKEEIDRYINLYQAIGMPTTLAELHLGDATYEELLKVGQQATIEGET
                     IHEMPFKISAEDVAAALLTVDRYVSNHQ"
     gene            complement(29099..30025)
                     /gene="cysK"
                     /locus_tag="VBC28_000670"
     CDS             complement(29099..30025)
                     /gene="cysK"
                     /locus_tag="VBC28_000670"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEO6814671.1"
                     /translation="MSKIYNSITDLIGNTPIIQLHHIVPEGAAEVYVKLESFNPGSSV
                     KDRIALAMIEDAEQKGILKAGDTIVEPTSGNTGIGLAWVGKAKGYNVIIVMPETMSIE
                     RRKIIQAYGAQLVLTPGSEGMKGAIAKAKEISAEQNAWLPLQFNNQANPEIHEKTTGR
                     EIIETFGEKGLDAFIAGVGTGGTITGVSRALKKVNPDVAIYAVEADESAILSGEQPGP
                     HKIQGISAGFIPETLATDSYDHIIRVTSDDAIETGRIIGGLEGFLAGISASAAIYAAI
                     EVAKQLGKGKKVLALLPDNGERYLSTSLYDFE"
     gene            complement(30116..30760)
                     /locus_tag="VBC28_000671"
     CDS             complement(30116..30760)
                     /locus_tag="VBC28_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001108146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HEO6814672.1"
                     /translation="MNYKTIKSDGIVEEEIKKSRFICHLKRVESEEEGRNYITQIKKA
                     HYKANHSCSAMVIGEKGDIKRSSDDGEPSGTAGIPMLTVLEKQGLTNVVAVVTRYFGG
                     IKLGAGGLIRAYSGSVANTIKEIGVVEVKEQIGIRIQLTYPQYQTFDNFLKEHHLQEF
                     ETEFLEAVTCKIYVDPKEFEHTITNLTEFYQGKALLTEEGSQIVEIPFKAETDK"
     gene            30816..32105
                     /locus_tag="VBC28_000672"
     CDS             30816..32105
                     /locus_tag="VBC28_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEO6814673.1"
                     /translation="MENYLGRLWTKAQLSEQLRKIAISLPSFIKKGSDYICTRCSSSV
                     AKNCQLPTGNYYCRECIVFGRVTSNENLYYFPQKTFSKTNSLKWKGELTPYQNEVSEE
                     LLKGISSKENLLVHAVTGAGKTEMIYHSVAKVIDTGGSVCIASPRIDVCLELYKRLSN
                     DFRCAITLMHGESPSYQRSPLTIATTHQLLKFYHAFDLLIVDEVDAFPYVDNPILYQG
                     VKQALKENGTSIFLTATSTTELERKVARKELKKLHLARRFHANPLVIPEMVWVSGIQK
                     SLQTQKLPPKLYQLINKQRQTRYPLLLFFPHISEGQVFTEILRQAFPMEKIGFVSSKS
                     TSRLKLVQDFRDNKLSILVSTTILERGVTFPSVDVFVIQANHHLFTKSSLVQISGRVG
                     RALERPEGLLYFLHDGKSKSMHQAIKEIKNMNHIGGF"
     gene            32105..32770
                     /locus_tag="VBC28_000673"
     CDS             32105..32770
                     /locus_tag="VBC28_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000128288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEO6814674.1"
                     /translation="MTCLLCHEIDLSQLTFVELMLLKPKQNVICQTCKGSFEALSREM
                     GCQTCCKQIPQKQCQDCIYWGKKGIEVNHFSLYRYNEAMKKYFSLFKFQGDYLLKDVF
                     TKEIKAALKKYKGYTIVPVPLSHEGYQNRQFNQVIAFLQSANIPYKNILSKKDGGKQS
                     ANNKEERLKQVQQFTLKNEAELGDNLLIVDDIYTTSATIAQIRKLLEEKGIKNIKSFS
                     LAR"
     gene            32847..33401
                     /gene="raiA"
                     /locus_tag="VBC28_000674"
     CDS             32847..33401
                     /gene="raiA"
                     /locus_tag="VBC28_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000599095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEO6814675.1"
                     /translation="MIKYSIRGENIEVTEAIREYVETKLSKVEKYFNEAQELDTRVNL
                     KVYREKTAKVEVTILIDSITLRAEDVSQDMYGSIDLVVDKIERQIRKNKTKIAKKYRE
                     KIPASQVFTTEFEAEPDEEAVSQRIVRTKNVNLKPMDVEEALLQMELLGHDFFIYTDA
                     EDNTTNVLYKREDGELGLIEAKGN"
BASE COUNT        11089 a         5363 c         6650 g        10429 t
ORIGIN      
        1 tatgaggata ttagggaaaa attaaaagga ttttgctctc tatgatacag aacaaaatcc
       61 tttgtttaaa ttaataagtc aacttttgga attccaaaag ttgactttta ttttttatta
      121 gatcagacaa tgtggcgctc aaaagcattg tcagaaatcc cttgggaaag aattaatttc
      181 gcccattctt gagcagagtg gagactgtgg tctttataat ttccacaaga ttcaatggtt
      241 gttccaggaa cgtcttccca agtgacccca ccagcaatag cctctagact agatttgatg
      301 actttagcaa tttcagtagc atcctgtttt ccccacataa tcatgtgaaa accagttcta
      361 caaccaaaag gagaacaatc aatcaaacca ttaattcgtt ggcggattaa cttcgccaga
      421 aggtgctcga tagtatgtaa accagcagta tcaatagcat tttcattagg ctgaataagg
      481 cggatatcaa agttagtaat gatgtctccg accggtccaa cttcttctga aattaaacga
      541 acgtagggtg ctttaacgat ggtgtgatct aattcaaaac tctctacgac aacttctttt
      601 gtcataagtt ttctcctttt gtattacatt ctagagacta aaggagatgt tgaaaatttc
      661 tcctaatccc tttaaataac attctaatca tatcataaat gtctataatt ttaaattgtg
      721 aaaacgcctt ttttatgata aacttaaaag tggactttct atatgaaatc tagaaaaaaa
      781 ttgaggtata aacatgttta atattatatt agcaatggtt tgtgcactca ttggtttgat
      841 tattggttat gtggctattt caatgaaaat gaaatcatca aaagaagctg cagaactaac
      901 tcttttaaat gcagaacaag atgccgttga tttacgtggt aaagcagaaa ttgaagcaga
      961 acacatccgt aaagcagcgg aacgtgaaag taaagctcac caaaaagaat tacttttaga
     1021 agcaaaagaa gaggctagaa aatatcgtga agagatcgaa aaggaattta aatctgatag
     1081 acaagagttg aaacagatgg aggcacgttt aacagatcgt gcatcatctc ttgatcgtaa
     1141 agatgaaaat ctatctaaca aagaaaaaat gttagatagc aaagaacaaa gtctaaccga
     1201 taaatctaga catattaatg agcgcgaaca ggaaattgct actttagaaa caaaaaaagt
     1261 agaagaattg tcacgcatcg cagagcttag tcaagaagaa gctaaagata tcattttagc
     1321 tgataccgaa aaagatttag ctcatgatat tgcgacacgt attaaagaag cagaacgtga
     1381 agtaaaagat cgatcaaaca agatagcaaa agacttgcta gcccaagcta tgcaacgttt
     1441 agctggtgaa tatgttactg aacaaacaat tacaactgtt catctccctg atgataatat
     1501 gaagggacgt atcattggtc gtgaaggacg taatattcgt actctggaaa gtttgacagg
     1561 tattgatgtt atcatagatg atacgccaga agtcgttgtc ttatcaggtt tcgaccccat
     1621 tcgtcgtgaa attgctcgta tgactttaga atctttgatt caagatggac gtatccatcc
     1681 agctcgtatt gaagagttgg ttgaaaaaaa tcgtttggaa atggatcaac gtatccgtga
     1741 gtatggtgag gcagcagctt atgagattgg tgcaccaaat ctgcatcctg atttgataaa
     1801 aattatggga cgtttgcagt tccgtacatc atatggtcaa aatgtacttc gtcattcagt
     1861 tgaagttggt aaacttgcag gtattttggc tggagaacta ggagaaaatg ttgatttagc
     1921 tcgtcgggct ggattcctcc atgatatggg gaaagctatt gatcgcgaag ttgaaggcag
     1981 tcacgttgaa attggtatgg aatttgctcg taagtacaaa gagcatccga ttgttgttaa
     2041 tactattgca agccaccatg gtgatgtaga acctgacagc gttattgcag taattgttgc
     2101 tgctgcagac gcccttagtt cagcacgacc aggtgcgcgt aatgagtcaa tggaaaatta
     2161 tatcaaacgt ctgcgtgatt tagaagaaat tgctaatggc tttgagggag tgcaaaatgc
     2221 ctttgcactt caagccggac gtgaaattcg tatcatggta caacctggta aagtttctga
     2281 tgatcaagta gttattatgt cgcataaggt acgtgaaaaa atcgaacaaa acttggatta
     2341 tccaggtaat attaaggtaa cagttattcg tgaaatgcga gctgttgatt ttgcaaaata
     2401 gtcaaaagga gagcttgtta gctctccttt tgttatagag ttgacatttg tcatattttg
     2461 aaatgacaaa aatatctaaa aagtttactt ctgatttttt atactatgat tatagtaaag
     2521 gagaaccaaa tggcaaaaga tataattaag gttgatcata tatttaaaag catcgggcaa
     2581 aaaacgattt tagaagatat atcatttagt atagcttcta accaatgtgt agcattgatt
     2641 ggacctaatg gtgcagggaa aacaactctg atgtcaactt tgttaggtga tattagtatt
     2701 tcttcaggat cattgacgat ttttaatctg cctgcacacc acaataggtt aaaatataag
     2761 gtggccatct taccacaaga aaatgtattg ccctcaaagt ttacagtgag agaactcatt
     2821 gatttccaaa ggtgtttatt tccagaagtg ctgccaatgt ctctgatttt agactatcta
     2881 cagtggagtg atacgcactt gcaacaattt acagagactt tatctggagg gcaaaaacga
     2941 cttttagctt ttgttttaac tttggttggt aagccccaat tactattttt agatgaacct
     3001 acttccggaa tggatacttc cacacgtcaa cgattttggg agctgattgc gacactaaaa
     3061 aaagaaggtg tcacaattgt ctattctagt cattatatcg aagaggtaga acatacagct
     3121 gataggattt tagtacttca taaaggaaag ttattacgcg atacaacccc ctttgccatg
     3181 aagcaagaaa aaaccgaaaa gttattcacc gttccgctta gttatcaaaa attattacct
     3241 acctatttga ttacagagtg tgaagccaag agtgatagta taacgtttgt tactggggag
     3301 gctgaagctg tatggaaaat actggcagat aatggttgtc ctattgaagc tattgagatg
     3361 accaatagaa ctttgttaaa tcgtattttt gagactacta aggaggtaaa acatgagaat
     3421 ctttaaagca ttattgaaaa tggaatttct tttgacaaaa cgtcaacttg ccaatctcat
     3481 tatggctata ggtatgccag tggctttttt tctatttttt tcaggattta tgggcgaagg
     3541 actcactaaa gccatcgaag ctatctacgt tcgtaattat atgataacga tggcaggatt
     3601 ctcaagtctt agctttgctt tttttacttt tccattttct atgaaagatg atcaactctc
     3661 aaaccgaatg caattattaa gacactcacc agtaccaatg tggcaatatt atctagcaaa
     3721 aattattcgt attttatttt actattgcct agcaataact gttgttttcc taactgggca
     3781 tattttaagg caagttagta tgccaataga acaatggatg cagagctttt tattgctatt
     3841 aggaggagca acatgtttta ttccttttgg attgctcgtc tcctacttta aaaatactga
     3901 actaatgtca atggtagcca atatttgtta tatgtcatta gctgttcttg gtggcatgtg
     3961 gatgccaatt acaatgttcc caaaatggtt gcaagcttta tcaaagttaa cccctactta
     4021 ccatttgact caagttatcc ttagtccgtt tgctaatagt tttgctggtt tttctcttat
     4081 tattctgata ggctatggta ttataatgtt agttatcgct tatctactat ctcagaagag
     4141 gcattcaatt tgaaaaataa aaaaaataac tatgatttaa tgtattttat tccgttagta
     4201 tttttgattt accccatagg agggattctc tattatcact accctttttg gactttattt
     4261 tttacgctag cttttgtagg agcatattta tactctgtta ttatcagggg cgaatcaaag
     4321 tatcacatga ttgcctggtc tacaatgttg acatatattt tctacatgac aatttttata
     4381 aatagtggtt tcatctggta tatttacttt ctgagtaatt tactcgtcta tcgctttaga
     4441 gataaattga agtcttttag attcattagt tttgcttgta ctctagccac agtggtattt
     4501 ttatgcttct ttaaggcatc agactttggt gatagaatca tgtttctaat tgttcctatt
     4561 ttttgtatcg gttacatgtg gattgctatt gaaaatagaa atagcgaaga acaaagagaa
     4621 aaaattgcag agcaaaatca gtatatcaat attttgtcag ctgaaaatga acgtaaccgt
     4681 atagggcgtg atttgcatga tagtttagga catacatttg ctatgatgac actaaagaca
     4741 gaactagcgt taaagttgtt agaaaaaagg aactatgata aggtacaaaa agaactatca
     4801 gaattaaatc atattagtca tcaatcgatg agtgaggtcc gtcaaattgt ttctaacctc
     4861 aagtatcgta cagtagtgga agaaattgat gagttgtacc gcttgtttca attatctaac
     4921 attaagttaa cagttgttaa taagttagag acgtcacaac taagtccagt aacacaatct
     4981 acaatcacaa tgatattaaa ggaattatcc aataatattg ttaagcatgc tgaagctgat
     5041 agcgtggagt tgagtttagt aaggcaaggt gctactataa atattgagat gattgataat
     5101 ggttgtggtt ttacaaacct tgacggagat gagttgcata gtattcaaga gagattaact
     5161 atcgttgaag ggacactaac gattttgtcc cgaagtaaac caactcatat tcaggtggta
     5221 ttgaaagagg tataagatga aattacttgt tgctgaggat cagtccatgc tacgagatgc
     5281 tatgtgccaa ttacttttga tggaagaaag tgtttctact attgatcaag ctggaaatgg
     5341 tggagaagct attgccattt tatccaataa agccatagat gttgcaatat tggatgttga
     5401 gatgccgata ttatcaggtt tagatgtctt agagtgggtt agaaaatacc agaatgttaa
     5461 ggtcataatt gtaacaacct ttaagcggtc aggttatttc caaagggcta ttcgttcaaa
     5521 tgtagatgct tatgttttga aagaccgctc agtagctgat ttgatgaaaa cgattcaaaa
     5581 agttttatct gggggtaaag aatattcgcc tgaattaatg gaaaacgtta tatctaatcc
     5641 tttgagtgag caagaaataa aaattctttc cctaattgct caagggaaga ccagcaaaga
     5701 aattgcatgc actttatttt tatcgaatgg tacagttcgt aattacacgt caaatatttt
     5761 tgataaatta ggagttaata atcgtttgga ggcacttaaa atagcacgag aaaaggattg
     5821 gctgtgatag tttttttcca gtcgagatgc cataatgtat taagagggat gtatgtatgc
     5881 aaaaatttaa agaacgtttt gatacgctta gtgatgcaat tttggccatt gctatgacga
     5941 ttttagtttt ggagataaaa actccggcta cgatgggtga tattggtgac tttacacgga
     6001 acataggttt atttattgtt agctttgttg ttgtttttaa tttttggtac gaaagggcac
     6061 aaaatagcct agatgctcaa aagactaatg atgaaattat agctcttgat atcattgaac
     6121 acctaggtat ttgtttgatt cctctcttta ctaaatttat gatttctttt gagaatcata
     6181 attttgctgt tatggcttat ggactcttaa cgttattagt tggtctcaca tcagacatta
     6241 ttcggattcg tttggcaagt tatgatttag tgaccattcc tagtgaattg aaagagcggg
     6301 ttataaaagt catgacaact tttgccattc gttcagttgt ggttcgcttt atcatcatta
     6361 tcctcgctta ttttctacct gaggtaggga tatttgcata tttagtcata ccgctcttta
     6421 tgttttggcg tcgctctaga gcaggaaaat actatgctaa aaagggcatt gaggcaccat
     6481 cttatatcaa gctttttatc caaattggag agagagctcc tgaggatttt actaattcaa
     6541 gtagtggaat aggtggggta aaaagactct ataaaaaata ataggagacc ttgaaaaaaa
     6601 ggtttctttt tgttaaacta atgagaagaa actttattta gggagcaatt tatgaattgc
     6661 tataaaagtt aatagttaag gtaagaacca gattgttctg gtctttttaa cacttaacac
     6721 ccgatctatt aatttgagga gtgtgtaatg tctgaacgtg gtttgctaat tgttttctca
     6781 ggaccatctg gtgttggtaa ggggacagtt aggcaagaaa ttttttcaac ccctgaccat
     6841 aaatttgatt attccgtttc aatgactact cgtccacaac gcccaggcga agttgatgga
     6901 gttgattatt tcttccgtac acgtgaagag tttgaagcgt tgattaaaga aggtcaaatg
     6961 cttgagtatg cggagtatgt tggtaactac tatggaactc ctttaagtta tgtcaacgag
     7021 actttagaca aagggattga tgtttttctt gaaatagaag ttcagggagc gcttcaagtt
     7081 aaatcgaagg ttccggacgg tgttttcatt ttcttaacac caccagattt agaggaatta
     7141 gaagagcgtt tagtaggacg tgggacagat agtccagaag tgattgccca acgtattgaa
     7201 cgtgccaaag aagaaattgc tttgatgagg gagtatgact atgctgttgt caatgatcaa
     7261 gtgtcattag cagcagaacg tgttaaacgt gtcattgaag cagaacatta tcgtgtggat
     7321 cgtgttattg ggcgttatac caacatggtc aaagaaactg ataaaaaatt aagttaaaaa
     7381 tagaaaagag aattgcctta tgatgttaaa accttctatc gacactttgt tggataaagt
     7441 tccttcaaaa tattctttag ttattttaca agctaagcgt gctcatgaat tggaagctgg
     7501 tgagaaagcc actcaagatt ttaaatctgt gaaatcaaca cttcgcgctt tagaagaaat
     7561 cgagtcaggt aatgttgtaa ttcacccaga tccatcagca aaacgtgcct cagttcgcgc
     7621 tcgtatcgaa gctgaacgcc ttgctaaaga agaagaagaa cgtaaaatca aagagcaaat
     7681 tgctaaagaa aaagaagacg gcgaaaaaat ttaagcaagt caaaagtgag aggttgaaac
     7741 attctgtatc agcctctttt catattttag agaattaggg agggtttatg gcaagaaaat
     7801 tagcacaggt tattgttgat attcctctga tgcagacaga taaaccattt tcgtatgcca
     7861 ttcctaaaga tttggaagat ttggtacaag taggagtacg cgttcatgtt ccttttggaa
     7921 gaggaaatcg tcttttgcaa ggctttgttg tcggtttcag agatgatgat gaattagaga
     7981 ctaaggatat tgcggaggtc ctagattttg aacctgtttt aaatcaggaa caactagact
     8041 tagctgatca gatgcgccat actgtctttt cttataaaat atctattttg aaatcaatgt
     8101 taccaagtct tttgaattcg caatatgata aactcttgtt agcaacagac acattaccat
     8161 cagaagatag agaagatcta tttggtcata aaacggaaat tgtcttttca agtttgtctt
     8221 ctcaagatgc caaaaaagca ggcagattaa ttcaaaaagg tttcatagaa gttcaatatt
     8281 tagccaaaga taagaaaaca ataaaaacag aaaagattta caaaatcaac agaactctgt
     8341 tagaaaagag ccaaatagca gcaagagcga aaaagagact ggagttgaaa gaattcctgt
     8401 tagaaaatcc tcaaccaggg cgcctcacag ctttaaacaa acaattttca tctcctgtgg
     8461 ttaatttttt cagagaagaa ggaatcattg aagtcattga aaaagaagct agtagatctg
     8521 ataattattt taaaggaatc ctaaaaacgg attttttgga tttaaatcag gaacaagcta
     8581 aagtagttaa aatagttgtt gaccaaattg gtaaggaaca gaataaaccc ttcttgttgg
     8641 aggggattac agggtctggt aagacagagg tatatcttca tatcattgat aacgttttaa
     8701 agttgggaaa aacagcgatt gttctcgttc cagaaatttc tttaactccc cagatgacga
     8761 atcgttttat ttctcgtttt ggaaagcaag ttgctattat gcactcgggc ttgtcagaag
     8821 gtgaaaaatt tgatgagtgg cgaaagataa aatcaggtca agctaaagtt gtcgttggag
     8881 ctcgttcagc aatttttgct cctttggaaa atatcggagc catcattata gatgaggaac
     8941 atgaatcaac ttataagcaa gaatctaacc caaggtatca tgcgcgcgat gtggccttgc
     9001 tacgtgcaga atattataaa gctgtgttac taatgggatc agcaactcca agtatcgaaa
     9061 gccgagctag agctagtcgg gatgtttata aatttttaga attaaaacat cgtgctaatc
     9121 ctaaagcacg tattcctcag gttgaaatta ttgattttag aaattttatt ggtcaacaag
     9181 aagttagtaa tttcacatca tatttactag ataaaatccg agatagatta gataagaaag
     9241 agcaagtggt tcttatgctc aatcgtagag gttattctag ttttattatg tgtcgagact
     9301 gcggctacgt tgatcaatgt cctaattgtg acatttctct gactttacat atggctacaa
     9361 agaccatgaa ttgtcattat tgtgggtttg aaaagcctat tccaagaaca tgtccaaatt
     9421 gcaatagtaa aagtatctct tactatggta ctggaaccca aaaagcttat gaagaacttt
     9481 tgaaagttat cccagacgca aagattttac ggatggatgt tgatacgaca aggcaaaaag
     9541 gcggtcatga gtctattttg aagcgtttcg gaaatcatga agctgatatt ttattgggaa
     9601 cacagatgat tgctaaggga ttggattttc caaatgttac tttagttgga gtactaaatg
     9661 cagatacttc tctgaattta cctgattttc gatcatcaga gcgaactttt cagctattaa
     9721 ctcaagtggc tggacgtgct gggcgtgctg aaaaagaagg agaggttgtc attcaaactt
     9781 ataatcctaa tcattatgct attcaattag cccaaaagca agattttgaa gctttttatc
     9841 aatatgaaat gaatatccgt cgacaacttg gttatcctcc ttattatttt acagttggtt
     9901 taacattatc gcataaagat gaagagtggt taatacgaaa atcttatgaa gtattaagtt
     9961 tattgaagca aggctttagt gataaagtaa agttgttagg tccaacgcca aaaccaattg
    10021 caagaacaca taacctttat cactaccaaa ttattatcaa gtatcgattt gaggacaacc
    10081 ttgaacttgt tttgaatcgc ttattagaca tgactcaaga caaggagaat agagatcttc
    10141 gcttagcaat agaccacgaa ccgcaaaata tgatgtagaa tagtgctatg atataattga
    10201 gaaaagaaag acgaggaaaa agcaatgaca aaattattat tcatggggac ccctgatttt
    10261 tcagcgacgg ttttaaaagg tattttagca gatggtaagt atgatgtttt agctgttgtg
    10321 acgcaaccag atcgtgctgt aggtcgtaaa aaagaaatta aaatgacccc tgtaaaagaa
    10381 gtggccttgg aaaacaacat tcctgtgtat cagccggaaa aactatctgg aagtccagaa
    10441 ttagaacaac taatgaccct aggtgcagat ggtattgtaa cagctgcttt tggtcaattc
    10501 ttaccaacaa agcttttgga atcggtaggt tttgcaatta atgtacatgc ttctctattg
    10561 ccaaaatatc gtggtggggc accaattcat tatgccatta ttaatggcga aaaagaagct
    10621 ggggttacca tcatggaaat ggttgcaaag atggatgcag gggatatggt atcaaaagct
    10681 tccgttgaga taactgatga agacaatgta gggacaatgt ttgatagact cgccgttgtt
    10741 ggtcgtgacc ttttattaga tacgcttcca ggttatctat caggtgatat taagccgata
    10801 ccacaaaatg aagaagaggt aagtttttct cctaatatta gtccagatga agagagaatt
    10861 gattggaata aatcatcgcg cgacattttt aaccatgttc gagggatgta tccatggcca
    10921 gttgcacata cccttttgga gggaaatcgt tttaaacttt atgaagtaac aatgtcagaa
    10981 ggaaagggct caccgggtca agtgatcgct aagacaaaga actcattgac agtagcaact
    11041 ggtgacggag ctattgaact caaatcagtt caaccagcag ggaaacctcg tatggatata
    11101 aaagactttt taaatggtgt tggtcgtaat ttagaaatag gagataaatt tggcgaatga
    11161 ttggaaaaag agtgcgcgtg gtttagcttt aatgacttta gaggaagttt ttgataaagg
    11221 agcttattct aatatcgcac ttaataagag tttaaaaaaa tctagactat cagataaaga
    11281 tcgtgcctta gtaacagaaa ttgtctatgg aactgtggct agaaaaataa cgctagaatg
    11341 gtatctatca cactttatag ttgatcgtga taagcttgaa ttatgggtat atcatctctt
    11401 gctactgagc ctttatcaac tactctatct ggacaacatc ccagatcacg ctattgttaa
    11461 tgatgctgtc actattgcta agaatcgagg caataaaaag ggtgctgaaa agttaattaa
    11521 tgccgtttta cgaagagtaa gttcggagac gctccctgaa attgcatcaa taaaacgcca
    11581 aaacaaacgt tactctgttg cttattccat gccagtgtgg ctcgtaaaaa aattaattga
    11641 ccaatatggt gagacaagag ctttagctat tatggagagt ttatttgagc gaaataaagc
    11701 cagtcttcgt gtgactgact tatcgcaaaa acaaactatc aaagaaactt taaatgttag
    11761 agattcacat attgcagaga cagctttagt tgcagactca ggaaattttg catcgacatc
    11821 atttttccaa gatggcctca ttactatcca agatgaatct agtcaattag ttgctccaac
    11881 actcaaagtc tcaggtaatg accaagtatt ggatgcatgt agtgcaccag gaggaaaaac
    11941 aagtcatatc gcctcatatt tgactacagg ggcagtaact gctttagatt tgtatgatca
    12001 taaattggaa ttggttatgg aaaatgcaaa acgcctaggt ctgtcagata agataaaaac
    12061 taaaaagtta gatgcaagta aagctcatga gtattttctt gaggatactt ttgacaaaat
    12121 tttagtagac gcaccttgtt caggtatcgg gctaattcgc cgtaaaccag atattaagta
    12181 caataaagct aatcaagact ttgaagcact ccaagagatt caattgtcta tcctatctag
    12241 cgtttgtcaa acgctacgta aaggtggtat aataacctat agcacttgta ccatttttga
    12301 ggaagaaaat tttcaagtta ttgaaaaatt cttagaaaac caccccaatt ttgagcaagt
    12361 agaactgagt catacacaag aggatattgt aaagagaggc tgtattagta taagccctga
    12421 acaatatcat acagatggct tttttattgg acaagtcaaa cgtatcttgt agtgtctagg
    12481 aggaaactga catcgtcaga aaaaaatata tggaaatatc tcttttaaca gatattgggc
    12541 aacgtcgttc caataatcaa gattttatta atcaatttga aaataaagcc ggcgtcccac
    12601 taatcatctt agcagatggt atgggaggac atcgtgcagg aaatattgct agcgagatga
    12661 ctgtaactga tctaggaagt gattgggcgg agacagattt ttctgagtta tcagaaattc
    12721 gtgattggat gcttgtctct attgaaacag agaatcgcaa aatatatgaa ttaggtcagt
    12781 cagatgatta caaaggtatg ggaactacca ttgaagcagt tgccattgta ggagataata
    12841 ttattttcgc acatgtagga gattcacgta tcggtatcgt tcgtcaagga gagtatcatt
    12901 tattgacgag tgatcattct cttgttaatg aattggtaaa agctggccaa ttaacagaag
    12961 aagaagctgc tagtcatcct caaaaaaata ttattacgca atcaattggt caagccaatc
    13021 cagtcgaacc tgatttagga gttcatctac ttgaagaagg tgactacctt gtagttaata
    13081 gtgatggact aactaatatg ttgagtaatg ccgatattgc tacagtacta acacaagaaa
    13141 aaaccttaga tgataagaat caagatttaa tcactctagc aaaccataga ggaggtcttg
    13201 ataatatcac tgtggcacta gtttacgttg aaagtgaggc ggtttaatga ttcagattgg
    13261 caaattattt gctggtcgtt atcggatcct taaatcaatc ggacgaggtg gaatggcgga
    13321 tgtttatcta gctcgagatc taattttaga taatgaagag gtcgcaataa aagtattgcg
    13381 aaccaattat caaacagatc agattgcggt tgctcgcttc cagagagaag cacgagcaat
    13441 ggctgagctg acacatccta atattgtcgc tattcgtgat ataggtgaag aagacggaca
    13501 gcagttctta gtaatggaat atgttgatgg attcgattta aaaaagtata ttcaagacaa
    13561 tgcaccccta tcaaataatg aagttgttcg aatcatgaat gaagtccttt cagccatgtc
    13621 actggcacat cagaaaggta tcgtacaccg tgatttaaaa cctcaaaata ttttattaac
    13681 taaaaaaggg actgtaaaag ttacagattt cggtattgcg gtagcgtttg ctgaaactag
    13741 tttaacgcaa acgaattcta tgttaggtag tgttcattat ttatcgccag agcaggcgcg
    13801 tggttcaaaa gcgactgttc agagtgatat ttatgccatg ggaatcatgc tttttgaaat
    13861 gttaactggc catattcctt atgatgggga tagtgcagtt actattgccc tacagcactt
    13921 ccaaaaacca ctaccatcaa tccttgcaga aaataaaagc gttccacaag ctttggaaaa
    13981 cattgtcatt aaagcaacag ctaaaaagct aacagatcgc tataagacaa cttatgagat
    14041 gggacgtgat ttatcaactg ctctaagctc gacacgtcac cgtgaaccaa aacttgtttt
    14101 taatgatact gagagtacta agacactacc caaagtaaca tctacagtat catctctaac
    14161 gacagaacaa ctgttgcgta atcaaaaaca ggccaaaaca acagaaaaaa taactcctga
    14221 ttctgcgagt aacgataaaa cgaagtctaa aaagaaggct tctcatcgac ttcttggtac
    14281 aatcatgaaa ttattctttg cactctgtgt tgtaggaata attgtatttg cttataaaat
    14341 tttagtaagt cctacgacta ttcgtgttcc agatgttagc aacaaaacag tagctcaagc
    14401 aaaaatgaca ttggaaaatt ctggattgaa agtaggagct attcgtaata ttgagagtga
    14461 tagtgtttca gaaggacttg tcgtaaaaac agaccctgct gcaggacgtt ctcgcagaga
    14521 aggggctaaa gttaatcttt atattgccac tccaaacaag agtttcactc taggtaatta
    14581 taaggaacat aattacaaag atatcctcaa agatcttcaa ggcaaaggtg ttaaaaaatc
    14641 tttaatcaag gttaagcgta aaattaataa tgattatact actggtacaa tcttagcaca
    14701 gtcgcttcca gaaggaacat cttttaatcc tgatggtaat aagaaattaa cacttacggt
    14761 tgctgttaat gatccaatga ttatgcctga tgtaacaggt atgactgttg gagaagtaat
    14821 agaaaccttg acagacctcg gattagatgc agataatctt gtcttctatc aaatgcaaaa
    14881 tggcgtttat caaacagtag taacacctcc atcttcaagt aagatagcaa gtcaggatcc
    14941 ttattacgga ggagaagttg gattaaggag aggagataaa gttaagctgt atcttcttgg
    15001 ttctaaaaca acgaataact catcatctac gccaatagat tcttcggctt caagctcaac
    15061 aggaacaact acctcggatt ctgtaagctc atcaacggat gctagcactt ctgatagtag
    15121 tagcacttct acaagtagtt caacactacc aagtgatagt acaactaata caggaacagc
    15181 taacaatcca ttaactcaat aaaattaaaa agaagatgtt aaagtcttct ttttaatttg
    15241 ctatggcaga gaccatgcct agaaaggttc tctgccaata tgccaaattt ttctctcaat
    15301 aatggtgcaa agtatagggc tttttgataa aatacgtcat atagagtaat ggaaggatta
    15361 ttatgaaaaa atttcagttt tttctacttg ttgaagccgt tgtcttagtt atgggattaa
    15421 tgaaaatttt atctgatgac tggacaagtt ttatttttat tctcgctttg attcttttag
    15481 ccttaagatt ttataacaac gatagtcgtc ataattttct gttaacgaca agtttgttat
    15541 tactattttt aatctttatg cttaaccctt acattattgc agccgttgtt tttgcagttt
    15601 tgtatgttct aatcaatcat ttctcgcaag ttaagaaaaa aaatcgttat gctttgattc
    15661 aatttaagaa tcatcaatta gatgttaaga caacacgaaa ccaatggtta ggtactgatc
    15721 aacacgaaag tgacttttat gcttttgaag atattaatat tataagaatc tctgggactg
    15781 atacaattga tttaacaaat gttattgtat ctgggcagga taatgttatt ataattcaaa
    15841 aagtttttgg tgatactaaa gtattggttc ctttagacgt tgccgtaaag gctgatatta
    15901 gttctgttta cggtagtgta cagtactttg attttgagga gtatgatcta agaaatgaat
    15961 ctataaaatt gtcacaagag gaagaatatt accttttgaa acgtgtcaaa ttagttgtaa
    16021 acactatcgc tgggaaagtt gaggttagtc gtaaatgaaa aagcaccact actttttagc
    16081 tttcttctat ggaagtgtca ttatatttgc catttgtttt gtcattattg atagtttggg
    16141 tgttaatttg gtgcacctct atcaaacgtc tcgtttgtgg ctgattgaac aattgatttt
    16201 ctctattttc tttctatctt tagcggtaac tatcctttta ttgctaactt ggtttttatt
    16261 agatgataat agtaaacgtc aaattaacca taatttacgt cgcattttaa ataaccaatc
    16321 aataaatgtt accgatgatg gtactgaaat atcgaccaat attcaacggc tctctaaaaa
    16381 aatgaattta atgacagcaa gtctacaatc taaggaaaat agtcgtattt taaaaagcca
    16441 agaaattgtg aaacaagaac gtaagcgtat tgcacgtgat ttacacgaca cagttagtca
    16501 ggatttgttc gctgcttcca tggttttgtc agggatagca cagaatgttt ctcagctaga
    16561 cgttgatcag gttggctcgc aattattagc agtagaagaa atgcttcagc atgcacaaaa
    16621 tgatttgaga attctgctac ttcatttgag gccagttgaa ttagaaaata agaccctatc
    16681 tgaaggattc agaatgatat tgaaagaatt gaccgataag agcgacattg aagttgttta
    16741 tcatgaatca attctaacct tacccaaaaa gattgaagac aatattttta gaattggtca
    16801 agaatttatc agtaatactt taaagcattc acaagccagt cgtctcgaag tttaccttaa
    16861 tcaaacagag aatgaattgc agttaaaaat gattgataat ggtattggat ttgatatgga
    16921 ttctgtctat gatttaagtt atgggttaaa aaatattgaa gatcgagtag aagatttagc
    16981 aggaaattta cagcttctca gtcagcctgg taaaggagtt gctatggata taagattacc
    17041 tttagtaaat cagagtgagg ataaaaatgg ataaaataaa aattgtactt gttgatgacc
    17101 atgagatggt tcgtctcggt ttaaaaagtt ttttaaactt acaagctgat gttgaagtaa
    17161 taggtgaggc ctcaaatggt ttagaaggca taaaaaaagc actggagtta cgtccagacg
    17221 ttgttgttat ggacttagta atgcctgaaa tggacggtgt agaagcaacc ttagcattat
    17281 tgaaggattg gccagaagct gccattttag tcttaacatc ttacttggat aatgaaaaaa
    17341 tttaccctgt tattgaagca ggtgccaaag gctatatgtt aaagacctca agcgcagcag
    17401 agattcttaa tgccattcgt aaagtttcaa ggggagaaca agctattgaa aatgaagtag
    17461 ataagaaaat taaggctcat gataaatgtc cagctttaca tgagggctta acagcaaggg
    17521 aacgtgatat ccttaattta ttagccaaag gttatgacaa tcaaaggatt gctgatgaat
    17581 tatttatctc gttaaagact gttaaaactc atgtttctaa tattttggga aaactaaatg
    17641 tagcagatcg aactcaagca gttgtctatg ctttccaaca ccatttggta ccacaagatg
    17701 atgaataagt gatataatag aggcactatg ttaatgaaag tggttagtca tggacgcaaa
    17761 aactaaatat aaagcaaaaa aaataaaggc agtgtttttt gatattgatg acacgcttcg
    17821 tgttaaagat acaggttata tgccaccatc aatcttaaaa gtttttaaag ctttgaaaga
    17881 taaaggaatc gttgtaggta ttgcttcagg aagagcacga tatggcgttc caaaagaagt
    17941 tcaagattta aatgccgatt attgtgtaaa attaaatgga gcttacgtta aggacaagga
    18001 taagaatatt atcttccatc gtcctattcc agctgaatat gtagagcaat ataagaaatg
    18061 ggcggacact gttggtatta agtacggttt agctggtcgt catgaggcag ttttatcaga
    18121 tcgtgatgat ttggtaaatg atgcgattga tattgtgtat tctgatctcg aagttaatcc
    18181 agatttcaat aaagaacatg atatttatca gatgtggaca tttgaagata aaggagatag
    18241 ccttcatcta cctgaaccgt tagctgaaca tcttcgcttg atacgttggc atgatcattc
    18301 ttcagatgtt gtgttaaaag gaacatcgaa ggctttaggt gtttctaaag ttgttgaaca
    18361 tttgggctta aagccagaga atatcttggt tttcggagat gagctcaatg atttagaact
    18421 ctttgattat gctggactag ctgtggcgat gggagtgtca cacccagaag ctcaaaaaaa
    18481 agcagatttt atcacaaaaa aagtagaaga agatggcatt ttgtatgctc tggaggaact
    18541 aggtttgata gaaaaagaat taacatttcc acaagtagat attgaaaata cagaaggtcc
    18601 tgtggctgtc attaaaacaa atcacgggga tatgactgta aaactcttcc cggatcatgc
    18661 tcctaaaacg gtagcaaact ttattggttt agctaaacaa ggttattatg atggtattat
    18721 cttccatcgt attattccag attttatgat tcagggtggt gatccaactg gtacaggtat
    18781 gggtggtgaa tctatctatg gggaaagttt tgaagacgaa ttttccgagg aattatataa
    18841 tgttcgcgga gctctttcaa tggctaatgc tggaccaaat acgaatggca gccaattttt
    18901 catcgtccaa aatacaaaaa ttccttatgc gaaaaaggaa ttagagcgag gtggttggcc
    18961 aacaccaatt gctgagttgt atgctggaca aggtggtaca ccgcatctag atcgtcgcca
    19021 cagtgtattt ggacaattag tagaccaaag ttcttttgag gtacttgatg agattgcggc
    19081 agtagagact ggttctcaag acaaaccatt ggaagatgtt gttattttaa cgattgaggt
    19141 taaagaataa aatgaagatt ggcgataagt taaagggaac agtcactggc attaggcctt
    19201 atggagcctt tgtttctctt gaggatggta ggactggact tatccatatt tctgaaatta
    19261 aaacaggtta cattgataat atttatgatg ttcttagtgt aggtgatgaa gtctatgttc
    19321 aggttataga tgtagatgaa tttacacaaa aagcaagttt atcgcttcgg acccttgaag
    19381 aagagagaca tcatattcag catcgtcatc gattttctaa caatcgtctt aagataggtt
    19441 tcaaaccact tgaggaaaat ttacctagtt gggttgagga aggtctagcc tacttgaata
    19501 ataaataaac aaaaaaccta gatttgatta atgacaagtc taggttttaa atatttaaaa
    19561 gtagcagtga atattatact tgctaaaaat agcttgataa tcgagtagat cctctgggtg
    19621 tagtgcagcg atctcttcca tttcatattg acgattaagt aattgatatt tgttttgccc
    19681 aaattggtgg aaaggtaaga gttgcacttg acggatatct aggcgatcaa ataaacaagc
    19741 aaattcttca gcatcttcta atgaatcgtt aaaattaggt atgacaggaa tacgtagcac
    19801 aatcgtttta ccgttggcaa aagcataatg aatgtttttg atgattgaag cattcttaac
    19861 catcgtcttt tcttggtgtt tgaggctatt gtaatgtttt aaatcggtgt aaataaaatc
    19921 aacgtattgg ataagatcta tgaattgttc atgtctagta taagcagttg tttcaatagc
    19981 cgtgtggatt cccagtgatt tagcacgttt taaaattgcc ttggcaaatt caaattgtgc
    20041 aaatatttca ccgccagata gcgttatgcc cccaccagat tcttcgtaaa aatcaatatc
    20101 ttttaaaact tcttcaataa tatcatcaac cgatttttct tcgcccacga tgacgctttc
    20161 attagtaatg gcatctctca ttgtttctgg aaccattttt tgcgactcgg gattagcaca
    20221 ccaaggacat ctaagaggac aaccctttaa gaaaacggtt gttcgaatac ctggaccatc
    20281 gtgaatacta aaatgttgaa tgttgaatac aatacctttt tcatttgtca tattattttc
    20341 ctcaatttca ttttaagtat accttttata cgtaacaaag tcaattacga atgaaagttt
    20401 attgaacttg tttaataaat aaaaaatgat aaaatagaaa ggaggtgttt catgaataga
    20461 ttagaaaata ttattagttt agtatcccag taccaaaaaa tagatgttaa tacactctcg
    20521 gagttgttgc aagtttctaa agtaacgata cgtaaagatt tagataaact agaaggcaaa
    20581 gggcttttgc atagagagca cggttatgca gttttaaaca gtggggatga ccttaatgtt
    20641 aggttatctt ttaaccataa aactaaaaaa gaaattgctg ccttagctgc aaatatggtg
    20701 tctgacaatg acactatctt aatagaatca ggctctactt gtgctctttt agcagaaaat
    20761 atctgtcaga caaagcgaaa tgtcactatt ttgaccaact cttgctttat tgctaactat
    20821 ttacgagagt atgattcatg ccaaattgtc ttacttggtg gtgagtatca atcaagttca
    20881 caggttacgg taggtcctct cttgaaaaag atgatttcac tttttcatgt ctcgttagct
    20941 tttgttggga ctgatggttt tgatccaaaa ctaggattta tgggtagaga tttaatgcgt
    21001 tctgaagttg cacaagagat ggcgaatgct gccgatgaag tcattatttt aacagattct
    21061 agtaagttta atcagactgc attggtggaa cagcttccgt tgtcaactgt atcacaagtt
    21121 attacagata aacatccaaa ttcggaaatt gctaatttgt tccaagaagc agagattacc
    21181 attcaatctt tattacaagt accataaaag aggctatttt tcttacattt tgcacatttg
    21241 tggcgataat agataaagtg tcatgtcaaa ttgcacagat gtaacctttt tgttaaactg
    21301 aagcaaatgc tgaaaggtta tgagtatgaa attagagcgt caaaaattgt tagctaaagt
    21361 agcttacctt tactatatgg aagggaaaag ccaatctgag attgccaatg aattaggaat
    21421 ttatcgtaca acaataagtc gtatgttagc caaagcgaga gaagaaggtc ttgttagaat
    21481 cgaaatttca gattttaatc cagaaatttt tcagttagaa tcctatttta aaagtaaata
    21541 tcaccttaaa gatattgaga ttgtttcttc gcgaaaagat agcgatactt cagagataga
    21601 gaaggattta gcacatgtag ctgcagctat gattcgaaaa aaaattaaag agaatgacaa
    21661 ggtggggatt gcttggggga ggacacttag taaagtagtt gaagcaatgc gtcctcatcc
    21721 ggtttctcaa gtttcttttg ttcctttggc cggaggtcca agtcatatta atgcacgcta
    21781 tcacgttaat actctggttt acgaaatgtc aaggcgtttc caaggaagtt gtactttcat
    21841 taatgcaact ctagttcaag aaaacgctaa tttagcgaaa ggaatattaa cttcaaaata
    21901 ttttgagggg ctaatggata attgggaaaa acttgatgtg gcaattgtgg gcgttggtgg
    21961 taaacctaaa agcaatgaac aacagtggct tgacctttta aatcaagatg attttcaatg
    22021 cttggatgaa gaagcagctg taggtgaaat tacttgtcgt ttttttaatc attcaggtga
    22081 tcctgtgaat caacatttag ctaaaaggac aataggaatt accttagaac aactacagaa
    22141 agttcctaat agaatagcgg ttgctcatgg caattataag gctgcggcac tcttagccgt
    22201 tttaaaaaag gggtatatta atcatttagt tactgatttt tccacagcct taaatatttt
    22261 aagattagat aaagacacct ttgtggatac tatatatcaa aagagctagg aataaatcct
    22321 agctcttttg ataatgtttc tattaccaag tgaattgtta tagaataagc acaaaggtgc
    22381 gctattgtca taaagggcac aaagtctatt gacgatgatt aagggacatg ttacatttgt
    22441 tttgcgaaag aaaactagaa ccttttgtaa gaaagtttaa acttataaaa aatcattaaa
    22501 gcggaggtgc ttatggaaat catagtcgca gaccaaatta ttatggggct cattttgaat
    22561 gctggggatg ctaaacaaca tatctatcaa gccttaaaac ttgcaaaaga aggaaatttt
    22621 gcagaaagta agattgaaat agaactagca gatagtgcac tcctagaagc tcataatctt
    22681 caaacacaat tccttgcaca agaagcagga gggacaagga cagatatctc agcactcttt
    22741 attcattctc aggatcattt gatgacaagt attactgaga ttaacttgat taaagaaatt
    22801 attgatttac gccaggaatt acatattcaa aaataagtaa aggggatcaa atatgataaa
    22861 aattggttta ttctgtgcag caggtttttc aacaggtatg ttagtaaaca atatgaaaat
    22921 cgcagcggat aaagaaggga tagaggcaca tattgaagct tattcacaag gaaaaattgc
    22981 agattatgct aaagatttag atgttgcatt acttggtcca caagttagct atactctaga
    23041 taaatcaaaa tcaatctgtg atgaatacgg tgtaccgata gcagttattc caatggcaga
    23101 ttatggtatg ttggatggtg taaaagtttt gaaacttgct ttgagtttat tggaaacaag
    23161 aaaagaggct tgatatgtca aagtttgata gtcagaaaat aattactccg attatgaagt
    23221 ttgtcaatat gcgagggatt attgcactca aagatggcat gctagcaatt ctaccactaa
    23281 cagttgttgg gagtctcttt ttaatattag ggcagcttcc atttaaaggt cttaatcaag
    23341 ccatagctaa tgtttttgga ccagaatgga cagaaccatt tatgcaagtt tattcaggaa
    23401 cttttgcgat tatgggcttg atttcctgtt ttgcaattgc ttatgcttat gctaaaaata
    23461 gtagtgtaga accgttacct gctggcgttt tatcactttc gtctttcttt attttaatga
    23521 aatcatctta tattcctgta aaaggagaag caatagcgga tgctatttct aaggtttggt
    23581 ttggcggtca ggggattatt ggagcaatta ttattggctt agtagttggt gctatctata
    23641 catggtttat ccaacatcat attgttataa aaatgccaga gcaagtacca caggcaatag
    23701 caaaacaatt tgaagctatg attccagctt ttgttatctt tcttttatcg atgattgttt
    23761 atttgattgc taaggtaaca actggaggta cctttattga gatgatttat gatatcattc
    23821 aagtaccgtt gcaaggctta acaggctcac tttatggagc aattgggatt gctttcttta
    23881 tttcattctt atggtggttt ggtgtccatg gccaatctgt ggtgaatggt attgtgactg
    23941 ctttgttact atccaattta gatgccaata agtctttatt agcagcaaat cgcttaacat
    24001 tagataatgg cgctcacatt gtaacacagc agtttttgga tagtttcttg attttatcgg
    24061 gctcaggaat aacttttgga ctagttattg cgatgctttt tgcagcaaaa tcaaaacaat
    24121 acaaggcact tgggaaagtg gccgcttttc ccgcaatttt caatgttaac gagccaatcg
    24181 tctttggctt tccaatcgta atgaatcctg tgatgtttct gccttttatt ttagtaccag
    24241 ttttggcagc tttaattgtt tatggagcta ttgcggttgg ttttatgcaa ccattctcag
    24301 gtgttacttt accatggagt actccagcaa ttatttctgg attcatggta ggtggttggc
    24361 aaggtgcact cgtccaaata gtcattttag ctatctcaac tgctgtttac ttcccattct
    24421 ttaaaatcca agataatatt acttacaaaa atgaatgtga aatggaaagg ggatagctag
    24481 cagataagag gttgaggtgt gtaatgcacc ttaacctttt tctaaaaatg cacatttgtg
    24541 caactttaga tgtagcattc tattctttat tttcttcttg ttattttagg atttaggtgc
    24601 tattatattt acgaagaaaa actttttagt ttcgaaagaa acaagcggag gctgttatga
    24661 ccaaacaatt actcactcaa aaatatacaa atgatgttca acaaaattct caaaagcatt
    24721 ttggctattt gacagagcgc atgtattctt atcgcgataa ggttcttgat aagaaaccat
    24781 tcatagatgc agaacgtgct atcttggtaa ctgaagccta tcaaaagcat caggaaaaac
    24841 ctaatgtttt aaagagagct tacatgttgc aaaatatttt ggaaaaaatg acaatctata
    24901 ttgacgatga gacaatgatt gtcggtaacc aagcttcatc tgataaagat gctcctattt
    24961 ttccagaata cactctagaa tttgttgtaa atgaactaga cctttttgaa aaacgagatg
    25021 gtgatgtctt ttacattaca gaagaaacca aagaacaaat tagaaatatt gcacctttct
    25081 gggaaaataa taatttacgg gctcgtgcgg gcgttatgct tccagaagaa gttcaagttt
    25141 atatggagac aggcttcttt gggatggaag gtaagatgaa ttctggggat gctcacttag
    25201 ctgtcaacta tcagaagtta ctagaagaag ggcttattgg ctttgaaaaa aaagcaagaa
    25261 aagctaaagc tgacttagat ttaacaaagc ctgagagtat tgacaaatat catttctatg
    25321 attctatttt aattacaata gaagctgtta aaacctatgc agagcgtttt gctatcctag
    25381 caaaaaaaca agctaaaaca gcaaatgcta agagacgtca ggaattgtta gatatcgcta
    25441 gtatttgtga acgagtgccc tattacccag ctgaaacgtt tgcagaagcc gttcaatcgg
    25501 tttggtttat tcagtgtatt cttcaaatag aatcaaacgg tcattctcta tcttatggac
    25561 gctttgatca atacatgtat ccatatgtta aatctgactt agaagctgga cgtgagactg
    25621 aggattcgat cgttgagcgc ctaactaacc tatggattaa aacaataact attaataagg
    25681 ttcgtagcca agctcatacg ttttcgtctg ctggtagtcc tctttaccaa aatgtaacga
    25741 ttggaggaca aacacgacat aaggaagatg ctgttaatcc tttatcattt ctagtactaa
    25801 aatcagtcgc tcagacacac ttaccacagc ctaatctaac ggttcgttat catgctaacc
    25861 tagacaagtc attcatgaat gaagctattg aagttatgaa attaggcttt ggtatgccag
    25921 cttttaataa tgatgaaatt attattccat catttattaa aaaaggagtt tccgaagaag
    25981 atgcctatga ttattcagcg attggttgtg tcgaaacagc agttcctggg aaatggggtt
    26041 atcgttgtac cggcatgagc tatatcaact tccctaaagt gttattaatt acaatgaatg
    26101 acggtattga cccggcttct gggaagagat ttgcgccttc ttatgggcat ttcacccaga
    26161 tgacctcata taaagaactt aaagaagctt gggacaaaac acttcgttac ttaactcgaa
    26221 tgagcgttat tgttgaaaat gctattgata ttagtctcga aagagaagta cctgatattc
    26281 tttgttcagc tttaactgat gattgtattg gacgaggtaa gcacttaaaa gaaggtggtg
    26341 cagtatatga ctacatatct ggtctacaag ttggaatcgc taatctttca gactcacttg
    26401 ctgcgcttaa aaaattagta tttgaagaaa aacgcctaac cacattagaa gtatggcaag
    26461 cattgcagag tgattacgca ggtccgcgtg gtgaagaaat tcgtcaaatg ttgatcaatg
    26521 aagcaccaaa atatggaaat gatgatgact acgcggatag tttagtacgt gagtgttatg
    26581 atgtttatgt tgaagaaatt gctaaatatc ccaacacacg ctatggacgt ggaccaatcg
    26641 gtggtattcg ctattcagga acgtcctcta tttcagcaaa tgtaggccaa ggacgaggta
    26701 ctttagcaac tccagatggt aggcatgcag gaactccatt agctgaaggt tgttcaccat
    26761 ctcataatat ggacaaaaag ggacctacct ctgttttgaa atcagtttcg aagttaccaa
    26821 ctgatgagat tgtcggcggt gtactcctta accaaaaagt caaccctcaa accttggcaa
    26881 aagaagaaga taaacaaaaa ttaattgcct tacttagaac attcttcaat cgccttcatg
    26941 gttaccatat ccagtataat gttgtctcac gtgagacttt gattgatgct cagaagcacc
    27001 ctgaaaaaca ccgcgatttg attgtgcgtg tagcaggtta ttctgcattc tttaatgtcc
    27061 tatctaaggc aactcaagat gatattattg cacgtacaga acatgctctt taggagatta
    27121 ttatggaatt tttacttgac actttaaatt tagaagctat aaaaaaatgg catcatatac
    27181 tgccactagc aggtgtaact tcaaatccca ccattgctaa gaaagaagga gatatacatt
    27241 ttttccaacg cattcgtgat gttcgagaaa ttattggacg tgaagcaagt ttgcatgttc
    27301 aagtagtggc caaagattat caaggaatat tggatgatgc tgctaaaata cgccaagaaa
    27361 cggacgatga catttatatc aaagtcccag ttacgccaga tggattagca gcaattaaaa
    27421 cgttaaaagc agaaggttat aatattacag ccacagcaat atacaccagt atgcaaggtc
    27481 ttttggctat atcagctgga gcagactacc tagcacctta ctttaaccga atggagaacc
    27541 ttgatattga tgcgacacaa gttattaagg aattagcgca agcaatagag cggacaggtt
    27601 cttctagcaa aattttagca gctagtttta aaaacgcttc acaagtaacg aaggccttat
    27661 cacagggtgc tcaatcaatc accgcgggac cagatatttt tgaatcagtc tttgcgatgc
    27721 catctattgc aaaagctgtt aacgactttg cagatgattg gaaagccagt caacactcag
    27781 aacatattta acagagaacg ccaacttttg aggctgtatc tttaacataa aattttttaa
    27841 agaagaaaga ggttgagaat gaaagttttc gcaagtccat cgcgctatat acaaggaaag
    27901 gatgctttat tccaaagtat tgaacatatc aaatctttag gtcagactcc acttatcctt
    27961 tgtgatgatg ttgtttataa tatcgttgga gaaaggtttt tatcttatct acaagacgat
    28021 ttgttaccac atcgtgtttc ctttaacgga gaagcatctg ataatgaaat caatcgtgtc
    28081 gttgctgttg cgaaagaaaa gaatagtgac cttattattg gacttggtgg tggaaaaact
    28141 attgactctg caaaggcaat tgcagataaa gttaatttac cagtggtcat tgcaccaaca
    28201 gtagcttcaa cagatgcccc aacatcagcg ttatcagtaa tctatacaga tgaaggagct
    28261 tttgaaaaat atatttttta ttcaaagaat ccagacttag ttttagttga tactcaagta
    28321 attgcccaag cgccaaaacg tttactagct tctgggatag cagatggctt agctacttgg
    28381 gttgaagcga gagcagtctt acaaaaaaat gggatagcga tggctggcgg tagacagact
    28441 ttagcgggcg tggcaattgc ccaagcatgt gagcgtactt tatttaatga tagtttacaa
    28501 gctttagcgg cttgtgatgc caaagtggtt acaaaagctt tagaaaatgt tattgaagct
    28561 aataccttac ttagtggttt aggttttgaa agtgcaggat tagctgcagc tcatgctatt
    28621 cataatggtt ttactgcctt gagtggagat attcatcact taacccatgg tgaaaaagtt
    28681 gcctatggaa cgttgacgca acttttcctc gaaaatagac caaaggaaga aattgatcgc
    28741 tacattaatc tttaccaagc gattggaatg ccaactacac ttgcagaact ccatttgggt
    28801 gatgcaacat atgaagaact tttaaaagtt ggtcagcaag caacaattga aggtgaaaca
    28861 atacatgaaa tgccattcaa aattagtgca gaggatgtcg ctgcagccct gttaacggtt
    28921 gaccgttatg tttcaaatca tcaataaaaa caatagaacc aagtgaaatt gcttggttct
    28981 atttgtcgtt ttaatatttt aactaaatag ggcttactag tatttaaaga gatataaact
    29041 aaaaaagatc agacaacata tctaatcttt tttagttatc gtttactgaa gattaaagtt
    29101 attcaaaatc atacaggctt gttgataagt aacgttcgcc attatcaggg agaagagcta
    29161 aaaccttttt cccttttcct agttgtttgg ccacttcaat agcagcataa atagcagctg
    29221 aagcagaaat tcctgcgagg aaaccttcta gtccgccgat gatacgtcct gtttcaatag
    29281 catcgtcaga tgtaacgcga atgatatgat cataagaatc tgtagccaat gtttctggaa
    29341 tgaaacctgc agaaatccct tgtattttat gaggcccagg ttgctcacca gaaaggattg
    29401 cagattcatc agcttctaca gcataaatag cgacatcagg attgactttt tttaatgcac
    29461 gagagacacc agtaattgtc ccaccagttc ccacacctgc gataaaagca tctaaacctt
    29521 tttcaccaaa agtctcgata atttccctac cagtcgtttt ttcgtggatt tctggattgg
    29581 cttgattatt aaattgcagg ggtagccaag cattttgctc tgcagaaatt tcttttgcct
    29641 tagcaatagc tcccttcatc ccttcactac caggtgttag tactaattga gcaccatagg
    29701 cttgaatgat tttacgacgt tcaattgaca tggtttctgg cataacaata ataacattat
    29761 accctttagc ttttcctacc caagccaaac cgatacctgt attaccagat gtaggttcta
    29821 cgattgtatc tccggcttta agaatccctt tttgctctgc atcttcaatc atagctagag
    29881 cgatacgatc tttgacagat gaacctgggt taaaggattc taacttaacg tacacctctg
    29941 cagctccttc tggaacaata tgatgtaatt gaataatggg tgtatttccg attaagtctg
    30001 taatagaatt ataaattttt gacataagct atacctcttc atgatattgg ttaagaatat
    30061 tataaaactc acattgatta aagtaaaata tcaattcttt atcaagtgat agctattatt
    30121 tatcggtttc agccttaaag ggaatttcaa caatttgact cccttcttca gttaacaaag
    30181 cttttccttg gtagaactct gtcaaattag ttattgtgtg ttcaaattcc ttagggtcaa
    30241 cgtatatttt acaggttaca gcttctaaaa attctgtttc aaactcctga aggtggtgct
    30301 cttttaggaa gttgtcaaaa gtttgatatt gaggataagt taattgaata cgaataccaa
    30361 tttgttcttt aacttctact accccgatct ctttgatggt attagctacg ctaccagagt
    30421 aggcacgaat aagcccccct gcgcctaatt tgatacctcc aaaataccgt gtaacgacgg
    30481 caacaacatt tgtcaggcct tgcttttcaa gtacggttag catagggata cctgctgtac
    30541 cactaggttc tccatcatca ctagatcgtt ttatatcccc tttttcgcca ataaccatag
    30601 ctgaacacga atgattggct ttgtagtgcg ctttcttgat ttgcgtaata taattacgcc
    30661 cctcctcttc ggactctact cgctttagat gacaaataaa tcgcgatttt ttaatttctt
    30721 cttcgacaat tccgtctgat tttatcgttt tatagttcat agagaaattc tacaaaaatt
    30781 cttcaaattt agcaaaaaaa tccgaatgaa ataatatgga aaattattta ggtaggcttt
    30841 ggacaaaagc acaattatct gagcaactta gaaaaattgc aattagcttg ccatctttta
    30901 taaagaaagg aagcgactat atttgtaccc gatgctctag ttctgttgct aaaaattgcc
    30961 aattaccaac tggaaattat tattgtagag aatgtatagt ttttggaaga gttactagta
    31021 atgaaaattt atattatttc ccacaaaaaa cattttctaa aacgaattct ttaaaatgga
    31081 aaggggaact gacaccttat caaaatgagg tttcagaaga gttgttgaaa ggtatttcaa
    31141 gtaaggaaaa tcttcttgtt catgctgtga caggggctgg taaaacagaa atgatatacc
    31201 actctgtcgc caaagtaatt gatactggag gaagcgtttg tattgccagt cctagaattg
    31261 atgtttgttt agaattgtat aagcgacttt caaacgattt tagatgtgcc ataacattaa
    31321 tgcatggaga atctccttct tatcagagat cccctttaac tattgccaca acacatcaat
    31381 tgttaaaatt ttatcatgct tttgatttgc taattgttga tgaagttgat gcttttccgt
    31441 atgtcgacaa cccgatacta taccagggtg taaaacaggc cttaaaggaa aacggaacaa
    31501 gtatttttct gactgcaact tcaacgactg agttagaaag aaaagttgca agaaaagaac
    31561 taaaaaaatt gcatctagct aggagatttc atgccaatcc gttagtgata cccgagatgg
    31621 tttgggtaag tggtatccag aaaagcttgc agacacaaaa actacctccc aaattatatc
    31681 agttgataaa taaacagcga caaacgagat atcctttgtt attatttttc ccccatattt
    31741 cagaaggtca ggtattcacc gaaattttaa gacaagcctt tcctatggaa aaaattggtt
    31801 ttgtttcaag taagagcaca tctaggttaa agttagtgca agattttcga gataacaaat
    31861 tgtctatcct agtgtcaact accattctag agagaggagt aactttccct agcgtcgacg
    31921 tttttgttat acaagcaaat catcatttgt ttacgaaaag ttccttggta caaatttcag
    31981 gaagagttgg aagagcatta gaaaggcccg aagggctcct ttacttcctt catgatggga
    32041 aaagtaaatc gatgcaccaa gctataaagg agattaaaaa catgaatcat ataggaggat
    32101 tttaatgact tgtttgttat gtcatgaaat agatttatcg caattaactt ttgtggaact
    32161 tatgcttcta aaaccaaagc aaaacgtaat ttgtcaaacg tgcaagggta gttttgaagc
    32221 acttagtagg gagatgggtt gccaaacatg ttgcaagcaa attccccaaa aacaatgtca
    32281 agattgtatt tattggggta aaaaaggtat agaggtaaat catttttccc tttatagata
    32341 caatgaagca atgaaaaaat atttttctct ttttaaattt caaggggatt atttgttgaa
    32401 agatgttttt acaaaagaaa taaaagctgc tctaaaaaag tataagggct acactatagt
    32461 gccagttccc ttgagtcatg aaggatacca aaataggcaa tttaatcaag tgattgcttt
    32521 tctacaatcg gcaaatatac cttacaaaaa tattctttct aaaaaagatg gaggtaagca
    32581 atcggctaat aataaagaag aaagactcaa acaagttcag caatttacat taaaaaatga
    32641 ggctgagtta ggggacaacc ttttaattgt tgatgatatt tatacaacaa gcgcaacaat
    32701 agcacaaatc aggaaactat tagaagaaaa aggtataaaa aatataaaaa gtttttcatt
    32761 agcacgctaa aatcttgcaa attttcatga aagcggttat aataagagta tagaaagcgt
    32821 taacaacgca agaaagaggt actcatatga ttaaatacag tattcgtggt gaaaacatcg
    32881 aagtaacaga agctattcgt gagtatgttg agaccaaact ctctaaagtt gaaaaatact
    32941 ttaatgaagc acaagaatta gatactagag tcaacctcaa agtatacaga gagaagaccg
    33001 caaaagttga agtaacaatc ttaatagatt ctattacttt acgagcagaa gatgtttcac
    33061 aagatatgta tgggtcaatt gacttagttg ttgataaaat tgagcgtcaa attcgtaaaa
    33121 ataaaactaa aattgcgaaa aaatatcgtg aaaagattcc agcaagtcaa gttttcacaa
    33181 cagaatttga ggctgagcca gatgaagaag cagtaagcca aagaattgta cgtactaaga
    33241 atgttaatct aaaaccaatg gatgttgagg aagcattgtt gcaaatggaa cttttaggtc
    33301 atgatttctt tatctataca gatgcagaag ataatacgac aaatgtcctt tacaagcgtg
    33361 aagatggcga actaggttta attgaagcta aaggaaatta actttgtaac ataatttaaa
    33421 aaatctcagg gttttctctg agattttttt aaaacctatt gacaacaaat aaaaaattat
    33481 agtatactaa atgagttctt aagcgaacct aatattgtat tatcatttta t
//
LOCUS       DATRMA010000015        30830 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.015, whole genome shotgun sequence.
ACCESSION   DATRMA010000015 DATRMA010000000
VERSION     DATRMA010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 30830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30830
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.015"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            <1..409
                     /locus_tag="VBC28_000675"
     CDS             <1..409
                     /locus_tag="VBC28_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO6814676.1"
                     /translation="QKVNHDAINDILDFLGLMDLKQVKCHSLSGGQQQKVAIAMAVLQ
                     KPKLILCDEISSALDTNSEKEIFNLLSDLREKYGISILMIAHHLSLLKQYCDRVMILD
                     HQTIVDTVVPVKATLNQLKSNYVDQVKEFLLHD"
     gene            402..1064
                     /locus_tag="VBC28_000676"
     CDS             402..1064
                     /locus_tag="VBC28_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6814677.1"
                     /translation="MIDIIEKHGEGILKSFWETNLMLGLTLILCFLIAFPTGILLFSL
                     RKSYLIKHSLAYQLLNLFLGTLRSVPFLIFIFILIPLNRLIFGTSFGTIAAILPLTLV
                     SVSLYARYVEQALLNIPQVVVDRALSLGANKRQIIYYFLIPSIKIDLVLSFTATAISI
                     LGYSTIMGVIGAGGLGEYAYRFGYQEYDYPVMYLIVVLFIIYVFILQSLGYFIANRYS
                     RK"
     gene            1080..1910
                     /locus_tag="VBC28_000677"
     CDS             1080..1910
                     /locus_tag="VBC28_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6814678.1"
                     /translation="MKKLLSLTCLIMMSLCLVACTKQAMSSKKIKDKNSKEKVITVAT
                     YSKPTSTFLDLVKDNVKEKGYTLKVVMVSDYIQANIALENKEHDANLLQHEFFMSIFN
                     KENDGHLVSITPIYHSLAGFYGQHLKNIAELKDGAKVAIPSDPANMTRALLLLQEKKL
                     ITLKNTSKKTKAIEDIITNPKKLRIEPVALLNLNQAYFEYDLVFNFPGYVTKINLVPK
                     RERLLYEKKPDIRFAGALVAREDNKNSDKIKVLKEVLTSKEIRHYITKEIPSEAAVAF
                     "
     gene            2145..3731
                     /locus_tag="VBC28_000678"
     CDS             2145..3731
                     /locus_tag="VBC28_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000673092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEO6814679.1"
                     /translation="MKFTELNLSQDILSAVEKAGFVEPSPIQEMTIPLALEGKDVIGQ
                     AQTGTGKTAAFGLPTLNKIHTEDNTIQALIIAPTRELAVQSQEELFRFGRDKGVKVRS
                     VYGGSSIEKQIKALRSGAHVVVGTPGRLLDLIKRKALKLNHIETLILDEADEMLNMGF
                     LEDIEAIISRVPETRQTLLFSATMPDPIKRIGVKFMKDPEHVKIKATELTNVNVDQYY
                     VRVKENEKFDTMTRLMDVDQPELSIVFGRTKRRVDELTRGLKLRGFRAEGIHGDLDQN
                     KRLRVIRDFKNDHIDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KSGQSITFVSPNEMGYLTIIENLTKKRMTGMKPATASEAFQAKKKVALKRIARDFEDQ
                     ELVSKFDKFKADALELATQYTPEELALYVLSLTVQDPESLPEVEITREKPLPFKPSGG
                     GFKGKGGRGNGRGGDRRRNDRGDRRGNRDRDDRGGRCDFKRRDDKFKKDNRRQENKKP
                     HKNTSSEKQTGFVIRNKGDK"
     gene            complement(3916..4182)
                     /locus_tag="VBC28_000679"
     CDS             complement(3916..4182)
                     /locus_tag="VBC28_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEO6814680.1"
                     /translation="MIKVPAYMYVLECSDGTLYTGYTTDVKRRLNTHNTGKGAKYTRA
                     RLPVKLLYSEAFNSKQEAMRAEALFKQKTRQAKLTYIKQHKNEQ"
     gene            complement(4175..4939)
                     /locus_tag="VBC28_000680"
     CDS             complement(4175..4939)
                     /locus_tag="VBC28_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HEO6814681.1"
                     /translation="MIDTILKENERIDQLFSTDVKIIQNKEVFSYSIDSVLLSRFPKL
                     PSRGLIVDLCSGNGAVGLFASTKTNATIIEIELQESLADMAKRSIKLNKLEKQVTMIN
                     DDLKNLLDHVQRSNVDLMLCNPPYFKASETSKKNLSPHYLLARHEITTNLREICQIAQ
                     HALKTKGRIAMVHRPDRFLEIIDTMRQFNLAPKRIQFVYPKLGKDANMLLIEAIKDGS
                     TEGMKILPPLVVHQDNGDYTETIFDIYFGENGKSHD"
     gene            5074..5814
                     /locus_tag="VBC28_000681"
     CDS             5074..5814
                     /locus_tag="VBC28_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEO6814682.1"
                     /translation="MFYTYLRTLVMFLIWVANGNAHYHNEDKMLKDDENYILVAPHRT
                     FWDPVYMAFAARPKQFIFMAKKELFTNRLFGWWIKMCGAFPIDREKPGQDAIRYPVKM
                     LKNSNRSLVMFPSGSRHSKDVKGGVAVIAKMAKVRIMPAAYRGPMVFKNLLKGHRVDM
                     NFGNPIDVSDIKRMDAEGIAEVSRRIQEEFDRLDRENETYDDGKKLNPLTYIYRLPLA
                     IIAIVLLVLTLIFSYLASFVWDPQKHLK"
     gene            5914..6567
                     /locus_tag="VBC28_000682"
     CDS             5914..6567
                     /locus_tag="VBC28_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEO6814683.1"
                     /translation="MFEIVLEKIKSHKWETTGIIVGLLLFGILGLNHFGTHHKEDNLN
                     INLEKKVSTITEKKVPMISHVKDKVSNQVTVDVKGAVNHPGVYSLPSQSRVTDAIKRA
                     GGLSNLADSKSVNLAQKLQDETVIYVAQKGEKITVVEEEKANNIATQGNSKGKINLNK
                     ADLSSLQTISGVGAKRAQDILDYRDSQGGFKTIDDLKNVSGIGEKTLEKLRQDVTID"
     gene            6551..8788
                     /locus_tag="VBC28_000683"
     CDS             6551..8788
                     /locus_tag="VBC28_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HEO6814684.1"
                     /translation="MLQLTKYFPLKPIYLALLVFQIYLLVFSWTMLGCAFLLFSFIFL
                     IYQYDRETIFKTIAIVIFFLFYFLWQNHNMNVQYQRVPNHISQIKVRIDTISINGDVL
                     SFQADASGNTYQAFYTLKNKSEKDYFQNLDNNIMIIADIKLEEAEERRHFNGFDYRQY
                     LKRHGIYRIAKVTKIKQIRLFQHRSFFALMSKWRRSAIVISQTFPNPMRHYMSGLLFG
                     YLDKTFDDMSDLYSSLGIIHLFALSGMQVGFFLGIFRYICLRIGLRLDHVWLLQIPFS
                     LIYAGLTGFSISVVRALIQSLLSHSGVKKDENFALCLLICLISLPHSLLTTGGVLSFA
                     YAFILTMTSFDHFSSIKKVAIESLTVSVGILPILTYYFSGFQPISIILTALLSFAFDI
                     IFLPLLTVIFVLSPIVKLSCINSLFEILEVLLKWTGQLFPRPLIFGKPSLFLLIVMII
                     ILGLLYDYYHSKCFRYCSLLIIFTLFFITKNPITNEVAILDVGQGDSILVRDWLGKTI
                     LIDTGGRVRFEQPEEWKQKVNQSNAKRTLIPYLKSRGISKIDDLVITHTDTDHMGDME
                     VISKHFKVARLITSSGSLTNSQYVKHLSKIGVAVKSIEAGDKLAVMGSYLQVLYPWHK
                     GDGKNNDSIVLYGHLLGKGFLFTGDLEEEGEKQLLEAYPNLSVDILKAGHHGSKGSSS
                     LSFLKKLSPSVVLVSAGKNNRYQHPHQETLQRFQKIKSKIFRTDQSGTIRLTGWWKWH
                     IQTVR"
     gene            8914..9723
                     /locus_tag="VBC28_000684"
     CDS             8914..9723
                     /locus_tag="VBC28_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000153214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEO6814685.1"
                     /translation="MTIKRIFCDMDGTLLNSEGQVSKSNATLIREAAIPVTLVSARAP
                     MEMKDAVDALQLGGVQVAFNGGLIYRIGDNNQVLPIHTQIIKKSTVKQLLRGIRFHFP
                     QVSLSYYDLNNWYCDKIDEGIRYEHSLTQQCPTFIHNEDQFLEGHTNTFKIMMITFDE
                     ANMLELEKYLQSLELPEITIQRSGKAYLEITHLLAKKSKGIAYILQKEQLAREETAAF
                     GDGHNDLPMLEMVGYPIVMDNAFDDIKAIAYQLTKSNDEDGVGYGIQKFLK"
     gene            9734..10678
                     /locus_tag="VBC28_000685"
     CDS             9734..10678
                     /locus_tag="VBC28_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEO6814686.1"
                     /translation="MTSISDIAKKAGVAKSTVSRVINHHPHVSDETRQKVMALITELD
                     YIPNQLARDLSRGKTQKIGVVIPHTRHPYFTQLINGLLDAAKTTDYQLVMMPSDYNQE
                     LELSYLKQLKMEAIDALIFTSRAISLDIIETYAKYGRIVVCEKLQEYNHLSSAYLDRY
                     SSFLEAFSDMKLRGLEHLVLLFSRNNESSATYQSALLAYQEVYGQLSSPYMVVGNVHD
                     FNDGLNLSYQLVKEVSIDGILATSDEVAAGLIKGYEESRKKCPYIIGQECLLVGQLLK
                     LPTIDHKSYYLGKLAFKQALAEKISQEVLFSEFLSQDN"
     gene            complement(10737..11729)
                     /locus_tag="VBC28_000686"
     CDS             complement(10737..11729)
                     /locus_tag="VBC28_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEO6814687.1"
                     /translation="MKNYRKLIVLLLLTFFVIFMGAYAYTHIVEKRSLTSNTIEKTLP
                     VVNQIKPQTIKEYQNYLTKVAKRNVLPVDIPQALNNEKVEITATDGMQTFTWNDKNNP
                     KQKVIFYVHGGSYIHQASELQYIFVNKLAKKLDAKVVFPIYPKAPTYNYSDAIPKIKK
                     LYQNTLASVTSPKQIILVGESAGGGLALGLADNLVTEHIKQPKEIILISPWLDIATNN
                     PKIEKVQKKDPLLKAWQLQQVAPYWANGKKNFKNPQVSPLYSSQFNKMAPISFFIGTH
                     DIFYPDNQLLHQKLAKENIKHHYIVGQKMNHVYPVLPIPEAETAFKQMLKIINH"
     gene            11905..12633
                     /locus_tag="VBC28_000687"
     CDS             11905..12633
                     /locus_tag="VBC28_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000468969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEO6814688.1"
                     /translation="MFHREDFSKALLKKALQPGMRVMDIGCGSGELTRLAADIVGKEG
                     DVVGIDINEQLLWQARHDNGYANVSYQNADLAALSSFETKSFDAIIGRRVLMYQKDPK
                     KVIESLTPLLKPNGFYVFQESDAMCLELAKPSFVNMNKIQSLIWQTVEKEGGHIHIGS
                     QLEQLMLEAGLIVDHFVSENVIQTSQTGTDLSWLLNIMKERIQKYSDLQGLEDVNLQK
                     ELEENNDHFIRDCQFGIISHRVDE"
     gene            12687..13724
                     /locus_tag="VBC28_000688"
     CDS             12687..13724
                     /locus_tag="VBC28_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEO6814689.1"
                     /translation="MIAIEEIGRITPDNLGLVTVLAGEDLGQYAQMKEKLFQVIGFNK
                     DDLAYSYFDLSEEDYQNAELDLESLPFLSDYKVVIFDQFQDITTDKKTYLDEQAMKRF
                     EAYLQNPVDTTRLVICAPGKLDGKRRLVKLLKRDARVLEANTLKESDLKTYFQKYAHQ
                     EGLVFEAGVFDELLIKSNYDFSDTLTNIAFLKSYKTDGHISSNDVREAIPKSLQDNIF
                     DLTQDVLLGRIDLARDLVRDLRLQGEDEIKLIAIMLGQFRMFLQVKILASKGKSESQI
                     VSELSHYIGRKINPYQVKFAVRDSRNLPLAFLKEAIRILIETDYAIKRGTYDKDYLFD
                     LALLKIAHKKP"
     gene            13804..14412
                     /locus_tag="VBC28_000689"
     CDS             13804..14412
                     /locus_tag="VBC28_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000974722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEO6814690.1"
                     /translation="MAIILPDLPYAYDALEPHIDAETMTLHHDKHHATYVANANAALE
                     KHPEIGEDLEALLADVSQIPEDIRQAVINNGGGHLNHALFWELMSPEETQISQELSED
                     INATFGSFEDFKAAFTAAATGRFGSGWAWLVVNAEGKLEVLSTANQDTPIMEGKKPIL
                     GLDVWEHAYYLNYRNVRPNYIKAFFEIINWNKVNELYQAAKA"
     gene            14750..15601
                     /locus_tag="VBC28_000690"
     CDS             14750..15601
                     /locus_tag="VBC28_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEO6814691.1"
                     /translation="MIIKRVLNHNAVISVTHQGLDVLLMGKGIAFKKRIGDRINSDAI
                     EKSFVLKNSDNMNRFTELFITVPEEVVACSERIINLGKIKLGKNLDEILYINLTDHIH
                     SAIERHEQGMVIQNPLRLEIQRYYPDEYSIGMKALELIKDELGICLTIDESAFIAMHF
                     VNAGLDNPFNEAHKITEIVSYIEQKVKIDFRTELDESSIDYYRFMTHTKLFAQRVLSG
                     MKYEDDDADLLLVVKKKYPREYKCVKEIGNNMAIQYQYQLNSSELLYLTVHVKRLVKN
                     LKEISDD"
     gene            15594..17462
                     /locus_tag="VBC28_000691"
     CDS             15594..17462
                     /locus_tag="VBC28_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HEO6814692.1"
                     /translation="MTKYQETAKAILAAVGGEKNIQHVTHCVTRLRLVLDNDEIVNDQ
                     VIKTIPNVIGVMRKNDQYQIILGNDVNNYYNAFLALGHFENTTREFSSQKKSSILEKL
                     IETIAGVITPLIPALLGGGMLKVIGILLPMLGIASSSSQTVAFINFFGDAAYYFMPIM
                     IAYSAASRFKVTPVLAATVGGILLHPAFVTMVAEGKPLSLFGAPVTLASYGSSVIPIL
                     IMVFLMQYIERWINKIVPSVMKSFLQPTLIILISGFLALVVVGPLGVIIGKGLSSAML
                     SIYHVAPWLALSILGAIMPLVVMTGMHWAFAPIFLAASVATPDVLILPAMLASNLAQG
                     AASLAVAVKAKQKQTRQVAFAAGLSALLAGITEPALYGVTLKFKKPLYAAMISGGLVG
                     AYIGLVNIASYTFVVPSIIGLPQYINPQGGNNFSNAVIAAIATIILTFIITWFLGIDE
                     GENEKSSINAQEHTHIRSGLSKKETLYSPMVGNVLPLSKVPDETFSSKLLGEGLAITP
                     SVGEVYAPFDGEIISLFPTKHAIALKDDKGVEVLIHIGIDTVELNGEGFEQLVKVGDF
                     VKRGQLLLRMDIDFISSKGYSLISPVVVTNSIDQLEIIVKDAETMVTNEDDLLVIL"
     gene            17479..18906
                     /locus_tag="VBC28_000692"
     CDS             17479..18906
                     /locus_tag="VBC28_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEO6814693.1"
                     /translation="MTVFPKHFLWGGAVAANQVEGAFRTDGKGLSVQDVLPNGGLGDF
                     TAKPTPDNLKLEAIDFYHNYKNDIKLFAEMGFKVFRTSIAWSRIFPNGDDSAPNEAGL
                     QFYDNLFDELLKYNIEPLVTLSHYETPLHLAKTYNGWADRRLIAFFEKFAQTVMERYK
                     DKVKYWLTFNEVNSILHMPFTSGAIMTDKSQLSPQELYQAIHHELVASARVTKLGRSI
                     NPNFKIGCMILAMPAYPMTSDPRDVLAARQFEQHNLLFSDIHVRGKYPTYIQSYFKNN
                     GIKIKFEEGDEEVLAQNTVDFLSFSYYMSVTQAYDFENYQSGQGNILGGLTNPHLTTS
                     EWGWQIDPIGLRLVLNQYYERYQIPLFIVENGLGAKDQLIETLDGDYTVEDDYRIDYM
                     NQHLVQVAKAIEDGVEIMGYTSWGCIDCVSMSTAQLSKRYGLIYVDRNDDGTGSLQRY
                     KKKSFGWYQKVIKTNGQSLFEHHNR"
     gene            complement(18975..20069)
                     /locus_tag="VBC28_000693"
     CDS             complement(18975..20069)
                     /locus_tag="VBC28_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO6814694.1"
                     /translation="MKLRKQLAQQIVTSIKDVCQQDINFINTKGIIFASTNPKRVGEF
                     HEIGLKVAQTGQMIEVTDQESYFGTQAGINIPFYYNCELLATIGISGNPNQVGKYALL
                     AQKMTRLILKEHELDYLDFGRKNEASIVLHHLVEGRELDYYYLNQFLNQYHLSEKTDY
                     RLLTFEINSQKQKLLLSQSEMSLLNFFDKLDTAIYTFNYPNQYWLLLSDHMFDYYYPN
                     ILSKFECEKGLYKVGIGQKSSLSLLKRSYETSILALKALKGQQKVNLVDDLDLELLLT
                     SIDSNIKQYVLNKALVNLSENDKILLNSYFKHNLSLKECSQELFIHKNTVQYRLNKIY
                     ESTQLNPRNFKDATLLYLALKSEDINLHKL"
     gene            20236..21378
                     /locus_tag="VBC28_000694"
     CDS             20236..21378
                     /locus_tag="VBC28_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000869902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEO6814695.1"
                     /translation="MKVVVAIDSLKGSLSSLEAGNAIKESINEVISGADVEVHPLADG
                     GEGTVEALTLGMGGTIETIPVKGPLGEKVHASYGIIPQRQLAIIEMAAAAGITLIATE
                     ERNPLHTTTYGVGEMIKDAISKGCRHFIIGIGGSATNDGGAGMLQALGYALLDKDNQE
                     ISLGAQGLADLKSISTDKVIEELKECDFKIACDVTNPLCGAQGCSSIFGPQKGADEDM
                     ITKMDTWLSNYATLATSVSEKADATIEGTGAAGGLGFAFLAFTNATLEPGIDIILSEI
                     NIEKAISEADLVVTGEGRLDGQTVMGKAPIGVAKLAKKYGKKVVAFSGSVTEDAILCN
                     QHGIDAFFPIVRRLISLDEAMSKEVAYKNMKETATQVFRLINLYNH"
     gene            21403..22659
                     /locus_tag="VBC28_000695"
     CDS             21403..22659
                     /locus_tag="VBC28_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HEO6814696.1"
                     /translation="MEATFTTTGALIGLALAILLIIKKVHPAYSLILGALVGGLIGGG
                     DLVTIVNTMVLGAQGMMSSILRILTSGILAGALIKTGSAEKIAESIIKKLGQQRAITA
                     LAIATMIICAVGVFIDIAVITVAPIALAIGKKANLSKSSILLAMIGGGKAGNIISPNP
                     NTIAASEAFKVDLTSLMVQNIIPAIAALVVTIILAKIVSKKNNDISYDSEEQVGSDLP
                     AFLPAISGPLVVICLLALRPLFGITIDPLIALPLGGLISILATGYLKETVPFVEYGLS
                     KVVGVSILLIGTGTLSGIIKASNLQFDMIHLLEFLNMPTFILAPLSGIFMGAATASTT
                     SGTTIASQTFAETLIKSGVPAVSGAAMIHAGATVLDSLPHGSFFHATGGAVNMAIKDR
                     MKLISYEALIGLTSTIVAVVYYCFFG"
     gene            22760..23824
                     /locus_tag="VBC28_000696"
     CDS             22760..23824
                     /locus_tag="VBC28_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEO6814697.1"
                     /translation="MKQTKKEFILGLTIIITLTLLIMLIFLVMFNKSSNSKLKQTKDR
                     RISSTSTTRTSSSSHDQKIVEKSLEKKKMVMEQDMELMNAYRLYYDYAHLSLKEVVLA
                     YMSEYGIEKNSVAFSYKNLKTGQRDAMNDTQAMTAGSTYKLPLNMLVVDAVVKGKLSM
                     EERFDITETDYEYIGEHNNYVAAFSGAMSIDDMQKYSLVYSENTPAYALAERIGGMDS
                     AYSKFGRYGQSKGDIKNIQKNGNKVTTDYYIQVLDYLWKHRKKYDSLITYLEEAFPTD
                     YYRALIPSDVVVAQKPGYVREALNVGAIVKEEVPYIVAIYTAGLGGSTQEDSEINGVG
                     LYQLEQLCFVINQWHRVNMN"
     gene            complement(23879..24214)
                     /locus_tag="VBC28_000697"
     CDS             complement(23879..24214)
                     /locus_tag="VBC28_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEO6814698.1"
                     /translation="MTYLQYVILLVLWEEDHRHMKELSYALSLNSNTLTPVLKRMENR
                     GWIVREKPRDDKRQLIIHLSNKGIESQDAVVNAVQSCGYFFSEISPESYQKTVSALSS
                     LNQVIKSHL"
     gene            complement(24403..25431)
                     /gene="queA"
                     /locus_tag="VBC28_000698"
     CDS             complement(24403..25431)
                     /gene="queA"
                     /locus_tag="VBC28_000698"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEO6814699.1"
                     /translation="MNTNDFDFYLPEELIAQTPLEKRDASKLLVIDHKNKTMTDSHFD
                     HILDELKPGDALVMNNTRVLPARLYGEKQDTHGHVELLLLKNTEGDQWEVLAKPAKRL
                     RVGTKVSFGDGRLIATVTKELEHGGRIVEFSYDGIFLEVLESLGEMPLPPYIHEKLED
                     RDRYQTVYAKENGSAAAPTAGLHFTKELLEKIETKGVKLVYLTLHVGLGTFRPVSVDN
                     LDEHEMHSEFYQLSKEAADTLNAVKESGGRIVAVGTTSIRTLETIGSKFNGELKADSG
                     WTNIFIKPGYQFKVVDAFSTNFHLPKSTLVMLVSAFAGRDFVLEAYNHAVEERYRFFS
                     FGDAMFVK"
     gene            25584..26264
                     /locus_tag="VBC28_000699"
     CDS             25584..26264
                     /locus_tag="VBC28_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT family protein"
                     /protein_id="HEO6814700.1"
                     /translation="MKEAKNFSQQLNILRAGVLGANDGIISVAGVVIGVASATHNLWI
                     IFLSAASAILAGAFSMAGGEYVSVSTQKDTEQAAVAREEKLLENNPELAKKSLVDIYL
                     AKGESHEHAQWLVDKAFSKNAIEHLVEEKYGIEFGEYTSPWHAAISSFIAFAIGSIFP
                     TITILLLPFSVRIVGTVIIVIVSLLSTGYVSAKLGQAPTVPAMRRNVMIGCLTMLATY
                     VIGQLFSI"
     gene            26415..27116
                     /locus_tag="VBC28_000700"
     CDS             26415..27116
                     /locus_tag="VBC28_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001263956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HEO6814701.1"
                     /translation="MRVITVKNDIEGGKIAFTLLEEKMKAGAQTLGLATGSSPITFYE
                     EIVKSNLDFSNMVSINLDEYVGIAASNDQSYSYFMHKHLFDAKPFKENNLPNGLAKDL
                     KEEIKRYDAVINANPIDFQILGIGRNGHIGFNEPGTPFDITTHVVDLAPSTIEANSRF
                     FNSIDDVPKQALSMGIGSIMKSKTIVLVAYGIEKAEAIASMIKGPITEDMPASILQKH
                     DDVVIIVDEAAASKL"
     gene            complement(27188..28144)
                     /locus_tag="VBC28_000701"
     CDS             complement(27188..28144)
                     /locus_tag="VBC28_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEO6814702.1"
                     /translation="MGLLVDGKWVDQWYDTASTGGKFVRTVTQFRHWVTKDGSAGPSG
                     DAGFKAESGRYHLYVSLACPWASRVLIMRKLKNLESHISISIVNPLMLENGWTFQEYK
                     GVIPDMINQSQYLYQIYQASQSDYTGRVTVPVLWDKKFHTIVNNESSEIMRMLNTAFN
                     HITGNTDDYYPDSLQGQIDEMNNFIYPKINNGVYKAGFATSQNVYQKEVETLFTALDQ
                     LEKHLSDNHYLVGEQFTEADIRLFTTLVRFDTVYYGHFKCNLKALHDYPHLWHYTKRI
                     YNLPGIAETVNFDHIKKHYYGSHKTINPTGIIPAGPNLDWTI"
     gene            28240..28959
                     /locus_tag="VBC28_000702"
     CDS             28240..28959
                     /locus_tag="VBC28_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEO6814703.1"
                     /translation="MRLDKLLGQAGFGSRNQVKKLICSRQVSVDGQIVTKDNVIVDSG
                     LQSIFVGKERVCLKESSYYLLYKPSGVVSAVRDSEHKTVIDLISEKDKVEGLYPIGRL
                     DRDTEGLLIVTNNGPLGYRMLHPKHHVAKTYYVEVNGFLERDAITFFEEGVVFDDGTK
                     CKPAELTIDTANNDKSTARITITEGKFHQVKKMFLAYGVKVIYLRRISFGDLRLDMNL
                     KPGQYRRLRDSEAAILKRYLD"
     gene            29032..30183
                     /locus_tag="VBC28_000703"
     CDS             29032..30183
                     /locus_tag="VBC28_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO6814704.1"
                     /translation="MNSKLSLKLTILSISIFLMSHLAIAPAIPKLYAFYHTQNPHIGL
                     ASVESLVTIPAMMITIFVILSNFVVTKLGKKNTVLLGLCLILMSGFISFFTSNFSLAM
                     ASRLLLGIGIGLYNSLSISIITDLYEADERASMIGLRTASLNIGKALTTFIVGLVLAI
                     GVNYIYLVYLLVIPVFFFFWKNVPEVENQTHTLKASTTFDTKAALLMLITFLVGIAYI
                     GATVKIPTLLVTKYHYATSFSSNMLTLLAFSGILVGSVFGKLVKVFQEKTLLIMILAM
                     GIGNVLFALANNQIIFIVASILIGASFVGTMSSVFFYISKNYAKEHNNFITSLALTAG
                     NIGVILTPLILTKLPSQLHLEPFMTPFLITSGLMVINVFVYLVLMSKNK"
     gene            30235..>30830
                     /locus_tag="VBC28_000704"
     CDS             30235..>30830
                     /locus_tag="VBC28_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000903651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HEO6814705.1"
                     /translation="MLIAKDKQGNLINLLESHPGKGQYFCPTCCSAVRLKAGRIMRRH
                     FAHISLKNCQFYHENESNEHLQLKAKLYMSLSRENETMLEHHLPEINQIADLFVNETL
                     ALEVQCSRLSEQRLRERTKAYLQADFQVRWLLGEKLWLKHRLTNLHKQFLQFSQSIGF
                     HIWELDLRLEVLRLKYLIYEDLRGHVYYLSKTCPLSGDV"
BASE COUNT        10018 a         4666 c         5908 g        10238 t
ORIGIN      
        1 tcaaaaagta aatcatgacg caattaatga tatcttagat tttttaggtt tgatggattt
       61 aaaacaggtg aaatgccata gtttgagtgg tgggcagcaa caaaaagttg caatcgctat
      121 ggctgttttg caaaaaccga aacttattct atgtgatgag atcagctctg ccttagatac
      181 taatagtgaa aaagagatat ttaacttgtt gtctgatctt cgtgaaaaat atggtatctc
      241 tatcttgatg attgctcatc acctatctct tttaaaacaa tattgtgatc gtgtaatgat
      301 tttggatcac cagactattg tagatactgt tgttccagtg aaagcaactc tgaatcaatt
      361 aaagtcaaac tacgtagatc aagtaaagga gtttttgtta catgattgac attattgaaa
      421 aacatggcga aggtatctta aaatcatttt gggaaaccaa tttaatgtta gggttaacct
      481 tgattctttg ttttctaatt gcttttccaa caggtatttt attgttttca ttgagaaagt
      541 cttacttgat taaacattct ttagcctatc aactattaaa tttattttta ggtacgttac
      601 ggagtgtccc tttccttatt tttattttta tcttaatccc tctcaatcga ttaatttttg
      661 ggacctcatt tggaacgata gcggcaatct tacctcttac tttggtttca gtcagtttgt
      721 atgcacgtta tgtggaacaa gctttattaa atatacctca agttgttgta gatcgagctc
      781 tcagtctagg ggctaacaag agacagataa tttattattt tttaatcccc tccatcaaga
      841 ttgaccttgt tttatcattt actgcaacgg ctataagtat tcttggctac tcgacaataa
      901 tgggagttat tggtgcagga ggtttgggtg aatatgccta tcgttttggc tatcaagagt
      961 atgactaccc agttatgtac ttgattgttg tcttatttat aatatatgtg tttatcttac
     1021 agagtttggg ctattttata gcaaaccgct actcaagaaa ataaaagaaa agaggaacta
     1081 tgaaaaaatt actttcccta acatgtctaa tcatgatgtc tttatgttta gtggcatgta
     1141 ctaagcaagc aatgtcgtct aagaaaatta aagataagaa tagtaaagaa aaggtgatta
     1201 ctgttgcaac ttacagcaaa cctacatcta cctttttaga tttggttaaa gataatgtaa
     1261 aagaaaaagg atatacttta aaggttgtca tggtctctga ctatattcag gctaacattg
     1321 ctttagaaaa caaagaacat gatgctaacc ttttacaaca tgaatttttc atgagtatct
     1381 ttaataagga aaatgatggt catctagtat caattacacc aatttatcat tcattggctg
     1441 gtttttatgg tcaacatttg aaaaatattg ccgagcttaa agacggtgct aaggtagcga
     1501 ttccgtctga tcctgccaat atgactagag ctctgctatt attgcaagaa aagaaactta
     1561 tcaccttaaa gaatacgtcc aaaaagacca aggctatcga agatattatt actaacccta
     1621 aaaaattacg aattgaacct gtagcattac ttaacctcaa tcaggcctat tttgaatatg
     1681 accttgtctt taatttccct ggatatgtga caaaaatcaa tctagttcct aaaagggaaa
     1741 gattattata tgagaaaaaa ccagatatcc gttttgcagg tgccttggta gctcgtgaag
     1801 ataataaaaa tagtgataaa ataaaagtac ttaaagaagt actaacaagt aaagagattc
     1861 gtcactatat cactaaggag attccaagtg aagcagccgt tgcgttctag atagataaac
     1921 tataaaccct ccttctttat agatggtggg tttttattgc cttttttcta ttttctgaag
     1981 caactaatag aactagcaat ttatgaaagc gtgttattta ttagattgac cttattttat
     2041 atctttttac ttttaattgt gttacactaa gtaggttgtc agaaaagata actaataaag
     2101 gttcataagg cagcttcgca ctgtctttta gaaaagagaa acatttgaaa tttacagaac
     2161 ttaacttatc gcaagatatt ctttcagcgg tagaaaaagc aggctttgta gagccatcac
     2221 caatccaaga aatgactatc ccacttgctc ttgaaggcaa agatgtcatt ggtcaagcgc
     2281 agacaggtac aggaaaaact gcagcttttg ggttaccaac tttaaataaa attcatactg
     2341 aagataatac tatccaagca cttattattg ctcctacacg tgaacttgca gtacaatcac
     2401 aagaagaatt gttccgtttt ggacgcgata aaggtgttaa ggtccgctct gtttatggtg
     2461 ggtcaagtat tgaaaaacaa atcaaggcat tacgttcagg agcacacgtt gttgttggaa
     2521 ctccaggtcg attacttgat ttaattaaac gtaaagcact taaacttaat cacattgaaa
     2581 ctcttattct tgatgaagca gacgaaatgc ttaacatggg atttttagag gacattgaag
     2641 caatcattag tcgtgtacca gaaacacgtc aaactctttt attctcagcg acaatgcctg
     2701 atccaatcaa acgtatcggc gttaaattca tgaaagatcc tgagcatgtt aaaattaaag
     2761 caactgaatt aactaatgtg aatgttgatc agtattatgt tcgtgttaag gaaaatgaaa
     2821 aattcgatac tatgacacgt ttgatggacg ttgatcaacc agaattgtca attgtctttg
     2881 gacgtacgaa acgtcgtgta gatgaattaa ctcgtggact aaaattacgt ggtttccgag
     2941 cagaaggaat tcatggagac ttagatcaaa ataaacgtct tcgtgttatc agagacttta
     3001 aaaatgacca tattgatatt cttgttgcca cagatgttgc tgcgcgtggt ttagatattt
     3061 caggcgttac acatgtttac aactatgata ttccccaaga tcccgaaagt tatgttcacc
     3121 gtattggacg tactggtcgt gctggtaaat ctggtcaatc tatcactttt gtttcaccaa
     3181 acgaaatggg atatttgaca attattgaaa acttaactaa aaaacgtatg actggaatga
     3241 agcctgctac tgcaagcgaa gcatttcagg ctaagaaaaa agttgcattg aaacgtattg
     3301 cacgtgactt tgaggatcag gaattagttt ctaaatttga taaatttaaa gccgatgctt
     3361 tagaattagc aactcaatac acaccagaag aacttgctct ttatgtctta agcttaacag
     3421 ttcaggatcc agaatcgtta ccagaagttg aaatcacacg tgaaaaacca ttaccattta
     3481 aaccatcagg tggtggcttt aaaggtaaag gtggacgagg caatggccgt ggtggcgatc
     3541 gtcgacgcaa tgatcgtggt gatcgtcgtg gaaatcgtga ccgtgatgat cgtggtggtc
     3601 gttgtgattt taaacgtcgt gatgataagt tcaagaaaga taatcgtcgt caagaaaata
     3661 aaaaacctca taaaaacact tcaagtgaaa aacaaactgg atttgttatt cgcaacaaag
     3721 gcgataaata agaggattat gaaaaaggag ttgctgagcg actccttttt tattttttgt
     3781 aagataaagc gtagatagtg atatccataa aattgcctta taactttaag aatgaagaca
     3841 aaagattaaa gtgaggttga aatttaaaag ttaaagaagc gaggataggc tcattacact
     3901 tagtatatta tcgtattatt gttcattttt atgttgttta atataagtca attttgcttg
     3961 ccgagtcttt tgtttaaata atgcttctgc cctcattgct tcttgtttgc tattaaaagc
     4021 ttcagaataa agaagtttta ctggcaatct cgcacgagta tatttagcac cttttccagt
     4081 attgtgtgta ttgagacggc gcttgacatc tgtagtataa cctgtataaa gtgttccatc
     4141 tgaacattct aaaacataca tataagcagg aactttaatc atgagatttt ccgttttctc
     4201 cgaaatatat atcaaaaata gtctcagtat agtctccatt atcttgatga acaacaagag
     4261 gaggaagtat tttcatacct tctgtcgagc catctttaat agcttcaatt aaaagcatat
     4321 tagcatcttt tcctaatttt ggataaacaa attggatacg tttaggtgct aaattgaact
     4381 gacgcatcgt atcaataatt tcaaggaaac ggtctggacg atgtaccata gcgatacgtc
     4441 cttttgtttt tagagcatgt tgagcaattt gacaaatctc tctaaggttt gttgttattt
     4501 cgtgtcttgc aaggagataa tgcgggctta gatttttttt agaggtttcg cttgctttga
     4561 aataaggtgg attgcaaagc attaaatcaa cgtttgaacg ttgaacatga tcaagaagat
     4621 tttttaaatc atcattaatc atagtaacct gtttttccaa tttattgagc ttaatcgaac
     4681 gcttagccat atccgcaagg ctctcttgta gttcgatctc tataattgtc gcatttgttt
     4741 tggtgctggc aaacaaaccg acagcaccat taccactaca taagtcaacg ataagaccac
     4801 gagaaggaag tttagggaac cgagacaata aaacactatc tatggaatag gaaaaaactt
     4861 ctttgttttg gatgattttg acatcagttg aaaaaagctg atcaatgcgt tcattttctt
     4921 ttaaaattgt atctatcata ggcttattat agcaaaatta cagcttccta gcactagttt
     4981 tgaaagtgtg gaggttgatt ttaagataat tctttctaaa aaaaaggaag catgataaaa
     5041 tagaagttac ttgatgaaag gtagaggaga aacatgttct atacttactt acgtacttta
     5101 gttatgttct tgatttgggt ggctaatggt aatgcgcact atcataatga agataaaatg
     5161 ttaaaagatg atgaaaatta tattttagta gctccacaca gaacattttg ggatcctgtg
     5221 tacatggctt ttgctgctag acccaaacag tttattttta tggccaaaaa agaattattc
     5281 acgaatcgtt tgtttggttg gtggattaaa atgtgtggtg ctttccctat tgatcgtgaa
     5341 aagccagggc aagatgctat tcgatatcct gtaaagatgc tgaaaaatag caaccgttct
     5401 ttggtgatgt ttccgagtgg aagccgccac tccaaagatg tcaaaggagg cgtagctgtt
     5461 attgcaaaaa tggcaaaagt cagaattatg ccagcagcat atagaggtcc tatggttttt
     5521 aaaaatctat taaaggggca tcgtgttgat atgaattttg gtaaccctat tgatgtttct
     5581 gatattaagc gtatggatgc cgaagggatt gcagaagtat ctcgtcgtat tcaggaagaa
     5641 tttgatcgtt tagatagaga aaatgaaact tatgatgatg gtaagaaatt aaatccattg
     5701 acttatattt atcgtctacc tttagcaatt atagctattg tcttgcttgt cctaactcta
     5761 atttttagtt atttagcaag ctttgtgtgg gatccacaaa aacatctaaa ataagggtta
     5821 catggaaact atttactttt tagtaaatag tttttttggt ttttaatagg aaaatctctt
     5881 tagttacgaa taagtaaatg gaggaatatt tttatgtttg aaatagtatt ggaaaaaatt
     5941 aaatcgcata aatgggagac aacaggaatt attgtaggac tcttattatt tggaattttg
     6001 ggtttaaatc attttggcac tcatcataaa gaagacaatt taaatatcaa tttagagaaa
     6061 aaagtatcaa ctataacaga aaaaaaggta ccaatgatta gtcacgtcaa agataaagta
     6121 tctaatcaag taacggttga cgtaaaaggg gcagtcaatc atccaggtgt ttatagtttg
     6181 ccttctcaaa gtcgtgtaac agatgcgatt aagcgtgcag gtggactaag caatctcgct
     6241 gatagtaaat ccgttaatct agcacagaaa ttacaagatg agacggttat ctatgttgct
     6301 caaaaaggtg aaaaaataac tgttgtggaa gaggagaaag caaataatat cgctactcag
     6361 ggaaatagta aggggaaaat taatcttaat aaagcagatt tatcaagtct tcagaccata
     6421 tcaggcgttg gggctaaaag agctcaagat attttagatt atcgtgatag tcaaggtggt
     6481 tttaaaacta ttgatgacct caaaaacgta tcaggtattg gtgagaaaac actagaaaaa
     6541 ttacgtcaag atgttacaat tgactaagta ttttcctcta aaacctattt atttagcatt
     6601 gttggtcttc caaatttact tactagtgtt ttcttggaca atgcttggtt gtgcctttct
     6661 tttattttct tttatttttc tgatttatca atatgatcgt gaaactattt ttaaaacaat
     6721 agcaatagta atttttttct tattttattt tttatggcaa aatcacaata tgaatgtcca
     6781 atatcaaaga gtaccgaatc atattagcca gattaaagtg cgtattgata ctatttctat
     6841 caatggtgat gttttatcat tccaggcaga tgcttcaggt aacacttatc aagcttttta
     6901 cacattaaaa aataaaagtg agaaagatta ttttcaaaat cttgataata atataatgat
     6961 aattgcagat atcaaacttg aagaagcaga ggagagaagg cattttaatg gctttgatta
     7021 tcgtcagtat ttaaaaagac atggaattta tcgtatcgcc aaagtgacaa agataaaaca
     7081 gatacgctta tttcaacata ggtctttctt tgctcttatg tctaagtggc gtagaagtgc
     7141 aattgttatt agtcaaactt ttccaaatcc tatgcgtcac tatatgtcag ggcttttgtt
     7201 tggatatcta gataagacct ttgatgacat gtccgattta tatagtagtc taggtattat
     7261 acatttattt gctttgtcag gtatgcaagt aggttttttt ctcggtattt ttcgttatat
     7321 ctgtctacgt attggcttac gtctagacca tgtttggtta cttcaaatac cattctcgct
     7381 aatttatgct ggtttaacag gctttagtat ctcagtcgtt agggcactta ttcaatcttt
     7441 attatcacat agcggtgtca agaaagatga gaactttgct ctctgcttgt taatttgtct
     7501 tatctccctc ccccactcac ttttgactac gggaggagtt cttagctttg cttatgcttt
     7561 tatacttacg atgacctcct ttgatcattt ttcgagtata aaaaaagtag ctatcgaatc
     7621 tttgacagtc tctgtaggaa ttcttcccat actaacctac tatttttcgg gttttcaacc
     7681 aatatcaatt atattaacag cacttttatc ttttgcattt gatattatat ttttgccttt
     7741 attaactgtt atatttgtct tatcgcctat cgttaaatta agttgtatta atagtttgtt
     7801 tgaaatccta gaagtgttat taaaatggac tgggcaactg tttccaaggc cacttatttt
     7861 tggaaagccc agcctttttc ttttaatagt catgattata attttgggat tactttatga
     7921 ttattatcat tctaaatgtt ttcgttattg ctcccttctt attatcttta ccttgttttt
     7981 tatcactaag aatccaatta ctaacgaggt tgcgatttta gatgttggac agggagatag
     8041 tattttagtg agggattggt taggaaaaac aattttaatt gatactgggg gaagggtgag
     8101 atttgaacag cctgaagaat ggaaacaaaa agtaaatcag tctaatgcta agagaacgct
     8161 cattccttac ttgaaaagca gaggtattag caagatagat gatttagtga taactcatac
     8221 cgatacagat catatggggg atatggaagt tatctcaaag cattttaaag ttgcacgttt
     8281 gattacaagt tcaggttctt taacgaattc gcagtacgtt aagcatttat caaagatagg
     8341 tgtagcggta aaatctatag aagccggtga taaacttgct gtcatgggaa gttatttaca
     8401 agtactttac ccatggcaca agggtgatgg aaaaaataat gattcaattg ttttatatgg
     8461 acatttatta ggaaaaggct tcttatttac cggtgatttg gaggaagagg gagaaaagca
     8521 gttattagaa gcttatccta atttatcagt agatatcctt aaagcaggac atcatggttc
     8581 taagggctca tcaagtctat cctttctgaa aaagttgtct cctagtgtgg ttctagtttc
     8641 agctggtaaa aataatcgtt accagcatcc tcatcaagag actttacaaa ggttccaaaa
     8701 gattaaaagc aagattttcc gaacggatca atcaggtaca attaggctaa caggatggtg
     8761 gaagtggcat attcagacag ttcgttgatt tataaaaatg attgcgtatc tgttaaatac
     8821 aatctatttt tcgcaagaat tcagtaattt taagcttgac atggaacggg ttccagcatt
     8881 tatactgatt tcagaagaag agaggtattt tttatgacta tcaaacgtat tttttgtgac
     8941 atggatggta cactattaaa tagtgaagga caagtgagta aaagtaacgc taccttaata
     9001 cgtgaggcag cgattccagt aactttagtg tctgctaggg cacctatgga aatgaaggat
     9061 gccgtagatg cacttcaatt aggaggagtc caagttgctt ttaatggagg attgatttat
     9121 agaataggag ataataatca agttctacct attcatacac aaatcattaa aaaaagcaca
     9181 gtaaaacaat tgctaagagg aatacggttt cattttcctc aagttagttt atcttattac
     9241 gatcttaata actggtattg tgataaaata gatgaaggaa tccgatacga acatagttta
     9301 actcaacaat gcccaacgtt tattcataat gaggatcagt ttttagaagg tcacacaaat
     9361 acttttaaaa ttatgatgat tactttcgat gaagcaaata tgctagaatt agaaaaatat
     9421 cttcaaagtt tggagttacc agaaatcact attcaacgtt caggtaaagc gtatcttgaa
     9481 attacacatc tcttagccaa aaaatctaaa ggaattgctt acatccttca aaaagagcag
     9541 ttagctagag aagaaacggc tgcatttgga gatggacata atgatttgcc tatgcttgaa
     9601 atggtaggtt accctattgt tatggataat gcttttgatg atatcaaagc gatagcatat
     9661 caactaacta agtctaatga tgaagacgga gtgggatatg ggattcaaaa atttttaaag
     9721 taggagcctt ataatgacaa gtattagtga tatagctaaa aaggcaggtg ttgctaaatc
     9781 aacagtttcc cgtgttatca atcatcaccc acatgtttct gatgagacgc gacaaaaagt
     9841 aatggctctt atcaccgaat tggattatat accaaatcaa ctagcgcggg atttaagtcg
     9901 aggcaaaaca caaaaaatag gtgtcgtcat tccccacacg cgccatcctt attttacaca
     9961 attaataaat ggcctattag atgctgctaa aactaccgac tatcaacttg ttatgatgcc
    10021 ttcggattac aaccaagaat tagaactttc ctacttgaaa cagctcaaga tggaagctat
    10081 tgatgcttta atattcactt caagagcaat tagcctcgat attattgaaa cttatgcaaa
    10141 atatggacga atagtagttt gtgaaaaatt gcaggaatat aatcatctat cctctgctta
    10201 ccttgatcgc tattcttcat ttctagaggc gttttctgat atgaagttac gaggtttgga
    10261 gcacttagtt ttattatttt caaggaataa tgaatcctct gctacctatc aatccgcatt
    10321 attagcttat caagaggtct atgggcaact atcctctcca tatatggtag ttgggaatgt
    10381 tcatgacttt aatgatggac taaatttatc ttatcaattg gtaaaagaag ttagtataga
    10441 tggtatatta gctacaagtg atgaagttgc ggcagggcta ataaaaggtt atgaagaaag
    10501 taggaaaaaa tgtccttata ttattggtca ggaatgttta ttagttgggc aacttttgaa
    10561 actcccaaca attgaccaca aatcctatta tttagggaaa ttagctttta aacaggcttt
    10621 agctgaaaag attagtcaag aagttttatt ctcagagttt ttatctcaag acaattagtc
    10681 attcaaaagt aatagaaaaa aggtttccaa ctaaacttga ttggaaacct ttttatttaa
    10741 tgattgataa ttttaagcat ttgtttgaaa gcagtctctg cttcaggaat cggtaagact
    10801 ggataaacgt ggttcatttt ttgaccaacg atatagtggt gtttaatgtt ttcttttgct
    10861 aatttttgat gtaataattg attatcgggg tagaaaatat cgtgcgttcc tataaaaaat
    10921 gatattggag ccatcttgtt aaattggcta ctgtaaagag gactaacttg aggattttta
    10981 aaattctttt ttccatttgc ccagtaagga gctacttgtt gtaattgcca agctttaaga
    11041 aggggatctt ttttttgaac tttttcaatc tttggattat tagtagcaat atctaaccat
    11101 ggtgatatta aaataatttc ttttggttgt ttgatatgct ccgtgacaag gttatcagca
    11161 agacctaaag caagacctcc gcctgcactt tcacctacta ggataatttg tttgggagat
    11221 gtgacgctag ctaatgtatt ttggtataat tttttaattt tggggatagc atcactataa
    11281 ttatatgtag gagctttagg gtaaatagga aagacaactt ttgcatctaa ttttttagct
    11341 agtttattga caaaaatata ttgtaattca gaagcttgat ggatatatga tcctccatga
    11401 acatagaaga taaccttttg cttaggatta tttttatcat tccaagtgaa tgtttgcatg
    11461 ccatcagtag cggtaatttc taccttttca ttatttaatg cctgaggaat gtctacagga
    11521 agaacattac gtttagctac cttagttaag taattttggt attctttgat ggtttgaggc
    11581 ttaatctgat ttaccacagg tagagttttt tcaatagtat tgctagttag ggatcttttt
    11641 tcaacaatat gcgtgtaagc atatgctccc ataaaaatga caaaaaaggt tagaagtagt
    11701 agtacaataa gttttcgata gtttttcatg attatccttt tgtaattgag ctcgtcatgc
    11761 taaaaatgat tagttgttat tctataataa agtagatacg aaaacaagaa aataatgcta
    11821 taaaatttca acctattata attttttgct gatagtacgt attaaccttt aatttgctaa
    11881 aatagaagag aggagagtgt tattatgttt cacagagaag atttttcaaa agcattatta
    11941 aaaaaagctt tgcagcccgg tatgagagtt atggatattg gttgtggtag tggggaattg
    12001 acgcgtttgg ctgctgatat cgttggtaaa gaaggtgatg ttgtaggaat tgatattaat
    12061 gagcaacttt tatggcaagc tagacatgat aatggctatg ctaatgttag ttatcaaaac
    12121 gcagatttag cagcactttc ctcctttgaa acaaaaagtt ttgatgcaat tattggacgt
    12181 cgtgtattaa tgtatcaaaa agatccaaag aaagtaatag aaagtttaac acctttgtta
    12241 aagccaaatg gtttttatgt ttttcaagaa agtgatgcaa tgtgtttaga actagctaag
    12301 cctagctttg taaatatgaa taaaatacaa tcactaatct ggcaaactgt tgagaaggaa
    12361 ggaggacaca ttcatattgg aagtcaactt gagcaattaa tgttagaagc aggcttaatc
    12421 gttgaccatt ttgtatcaga aaatgtcatt caaacgtcac aaactggtac agatttatct
    12481 tggctactaa atatcatgaa agaaagaata caaaaatata gtgaccttca gggattagaa
    12541 gatgtcaatt tacaaaaaga attagaagag aataatgacc attttatccg agattgtcaa
    12601 tttggaatta tttctcatag agttgatgaa tgattttaag tattatttga caccttgaaa
    12661 agtttcctta tggtataata aggaatatga ttgcgattga agaaattggg agaattaccc
    12721 cagataattt agggttagta actgttttag caggagaaga tttagggcaa tatgctcaga
    12781 tgaaagagaa gctatttcag gtaattggat tcaataaaga cgatttagcc tattcttatt
    12841 ttgatttaag tgaagaagac tatcaaaatg cggaattaga cttagaaagt ttaccttttt
    12901 tatcagacta caaggttgtt atttttgatc agtttcaaga tattacaact gataaaaaaa
    12961 cttatttaga tgaacaagca atgaagcgct ttgaagccta cttgcagaat cctgtagata
    13021 ctacgagact tgttatttgt gctccaggaa aacttgatgg aaaacgtcga cttgtaaagt
    13081 tattgaaacg tgatgctcgt gttttagagg ctaatacttt aaaagaatca gatttaaaga
    13141 cgtattttca aaaatatgct caccaagaag gattagtttt tgaagcaggt gtttttgatg
    13201 agttattgat taagtctaac tatgatttta gtgatacttt aacgaatatc gctttcttaa
    13261 agtcatataa aacagacggt catatttcgt ccaatgatgt tagagaggct atccctaaaa
    13321 gcctgcaaga taatatattt gatttgactc aagatgtctt acttggcagg atagatttag
    13381 cgcgtgattt agtaagagat ttacgtctac aaggcgaaga cgaaatcaaa ttgattgcaa
    13441 tcatgttagg acagtttcgg atgtttttgc aagttaaaat attggctagt aaagggaagt
    13501 cagagagtca aattgtttct gaactatccc attacatagg acgtaaaatc aacccttatc
    13561 aagtaaaatt tgctgtcaga gattctagaa accttcccct tgctttttta aaagaagcga
    13621 ttaggatttt aatcgagact gactatgcta ttaaaagagg gacttatgat aaggattatc
    13681 tctttgattt agccttatta aaaattgctc ataaaaagcc ctaattaaag aaacaaaaga
    13741 gttgaaagct gttccaaatt cctatagaat aagggtaata atagatgaaa agaggatttc
    13801 gacatggcaa ttattttacc agatttaccc tatgcatatg atgcgcttga gccacatatt
    13861 gatgctgaga caatgacact acatcatgat aagcaccatg caacttatgt tgctaatgca
    13921 aatgctgctc ttgagaaaca tcctgaaatt ggagaagact tagaggcgct cttagctgat
    13981 gtttctcaaa ttccagaaga tattcgtcag gcagtcatca ataacggtgg tggacatctt
    14041 aaccacgctc ttttctggga attgatgtca ccagaagaaa ctcaaatttc acaagagtta
    14101 tctgaagaca ttaatgcaac ttttggttca tttgaagact ttaaagctgc tttcacagca
    14161 gcagcaacag gacgttttgg ttcaggttgg gcttggcttg ttgttaatgc tgaaggcaaa
    14221 cttgaagtgc tttcaactgc caatcaagat actccaatta tggaaggcaa gaaacctatt
    14281 ttagggcttg atgtatggga gcatgcttat tacttaaact atcgtaatgt tcgtcctaac
    14341 tacatcaaag ctttctttga aattattaac tggaataaag taaatgaact ttaccaagct
    14401 gctaaagctt aataaaaaac gagtcgatag gctcgttttt ttattattct aaaagttgat
    14461 aatgtttttt aaaagtctag aagtccaaac tcagataaaa ctagaatagt cctattatct
    14521 tcaactaagc gaataagttt attgaaagcg cttgacaaga ttagtaagaa atgataaaat
    14581 taagacgttt tcaggtgtta ttatttaagg taagcaagct gataacagat aaaattgcat
    14641 aataaaggat gttatcacat tttgtgagca gggacctatt caacgacgac tttttgttgt
    14701 gtcttggatg ggtcattttt tattgtgcga gtgaaggact aggactttaa tgattataaa
    14761 acgcgtatta aatcataatg cagtcatatc agtaacacat caaggattag atgtgctact
    14821 gatggggaaa ggaattgcat ttaagaaaag aataggtgat aggattaata gcgatgctat
    14881 tgagaagtca tttgttttaa aaaatagtga taacatgaac cgttttacgg agttatttat
    14941 tactgttcca gaagaagtag ttgcatgttc agaacgaatt atcaacctag gtaaaatcaa
    15001 attgggtaag aacttagatg aaattcttta tatcaatttg acagatcata ttcattcggc
    15061 tattgaaaga catgaacaag gaatggttat ccaaaatcca ctaaggttgg aaatccaacg
    15121 ttactatcct gatgaatact ccattgggat gaaggctctg gaacttataa aggatgaatt
    15181 gggcatttgt ttaacaatcg atgagtcggc ttttattgct atgcactttg taaatgctgg
    15241 attggataat ccgtttaatg aagcgcataa aatcactgag attgtatctt acattgaaca
    15301 gaaagtaaaa atagattttc gaacagaact agatgaaagt tcaattgatt attatcgttt
    15361 catgacacat accaaattat ttgctcaacg cgttttatca ggaatgaaat acgaagatga
    15421 tgatgctgat ttacttttgg tagttaagaa aaaatatccg agagaatata aatgtgttaa
    15481 agagatcggc aacaatatgg ctatccaata ccaatatcaa cttaattcaa gtgaactcct
    15541 atatctgacg gtgcatgtta aacgattagt taaaaattta aaggagatat cagatgacta
    15601 aataccaaga gactgctaaa gctattttag cagcagtagg gggagaaaaa aatattcaac
    15661 acgtaaccca ctgtgtgaca cgattacgat tagttttaga caatgatgaa attgtcaacg
    15721 atcaggttat taaaactatt ccgaatgtta tcggtgttat gcgtaaaaat gatcaatacc
    15781 aaattatttt gggaaatgat gtcaataatt attataatgc ttttttggcc ttaggtcatt
    15841 ttgaaaatac taccagagaa ttttcatcac aaaaaaagag ttcaatactt gagaaattaa
    15901 ttgaaacgat tgcaggagtc attacgccgc ttatacctgc tcttctagga ggagggatgc
    15961 tcaaggttat aggtattttg ctccctatgc tcggtatagc aagctctagt tctcaaacag
    16021 tagcttttat caattttttt ggtgatgccg cttattattt tatgccaata atgattgctt
    16081 attcagctgc gtcacgattt aaagttacac ctgtattagc tgctacagta ggagggattc
    16141 tcttacaccc tgcttttgtg acaatggtag ctgaaggaaa accattatct ttatttggag
    16201 ctccggttac actggctagt tatggttctt ctgttattcc aatcttaatt atggtttttc
    16261 ttatgcaata tattgagaga tggattaata aaattgttcc tagtgtaatg aagagttttt
    16321 tacaaccgac tttgattatt ctcatttctg gctttttagc tttagtggtc gtggggccac
    16381 tgggggtaat tattggtaaa ggattgtcta gtgcaatgct ttcgatttac catgtggcgc
    16441 catggttagc tttatctatt cttggtgcta ttatgccatt ggttgttatg acaggaatgc
    16501 actgggcatt tgctccaata tttttagctg cttctgttgc gacaccagat gttttgattt
    16561 taccagcaat gttagcttct aatttagctc aaggtgcggc ttctcttgct gttgcagtta
    16621 aggctaaaca aaaacaaaca cgtcaggtgg cctttgcagc aggtttatca gcactactag
    16681 ctggtatcac agaacctgca ctttatggtg tcacactgaa attcaagaag cctttatacg
    16741 ctgcaatgat atcaggtggt ttagtaggag cttatattgg attagttaat attgcttcct
    16801 atacatttgt agtaccatct attattggtt tgccacaata tatcaatcca caggggggca
    16861 ataatttcag taatgctgtt attgcagcaa tagctactat tattttgact ttcattatta
    16921 catggttctt gggaattgat gaaggagaaa acgaaaaaag tagtattaat gctcaagagc
    16981 acacacatat tagaagtggt ttatcaaaaa aagagacatt gtattcacct atggtaggca
    17041 atgtattacc tttatctaaa gtacccgatg aaacattttc atctaaatta ctaggtgaag
    17101 gtttagcaat cactcctagt gtgggtgaag tttatgcacc atttgatggt gaaattataa
    17161 gtttatttcc tacaaaacat gccattgcct taaaggatga taagggagtc gaagtgttaa
    17221 tccatattgg aatagatact gtagaactaa atggagaagg ttttgaacaa ttggtcaaag
    17281 ttggcgattt tgttaaacga ggacagttac ttttgcgaat ggatattgat tttattagtt
    17341 caaaggggta ttctcttatt agtccggttg tagttacaaa ttcaattgac caacttgaaa
    17401 ttatcgttaa agacgcagag acaatggtta caaatgaaga tgatttactt gtaattcttt
    17461 aaaaggaagg atgataatat gacggttttt cctaaacatt ttctatgggg aggtgcagtt
    17521 gctgccaatc aggtagaggg tgcatttaga acagatggca aaggtttatc tgtacaagat
    17581 gtacttccaa atggtggttt aggcgatttc accgccaaac ctactccaga caatttgaag
    17641 ctagaagcaa ttgattttta tcataactat aaaaatgata taaaactgtt tgcagagatg
    17701 ggctttaaag tttttagaac ctctattgcc tggtctcgaa tttttccaaa tggtgatgat
    17761 agtgctccaa acgaagctgg cttacaattt tatgataatt tatttgatga gttgcttaaa
    17821 tataatatcg agcccttggt taccttatct cattatgaaa caccgcttca tcttgcaaaa
    17881 acttataatg gttgggctga tagacgtttg atagcatttt ttgagaaatt tgctcaaaca
    17941 gtcatggagc gttataaaga taaggtgaaa tattggttga cttttaatga agtgaattct
    18001 attttacata tgcctttcac cagtggtgct attatgaccg acaaatcaca actaagtcct
    18061 caggagcttt atcaagctat tcatcatgaa ttagtagcat cggctagagt gacaaaatta
    18121 gggcgttcta tcaatcctaa ttttaaaatt ggctgtatga ttctggctat gccagcttat
    18181 cctatgacat ctgatccaag agatgtcctg gctgcaagac aatttgaaca acataatctg
    18241 ctattttcag atatacatgt cagaggtaaa tatcccactt atattcagtc ctatttcaaa
    18301 aataatggta ttaaaattaa atttgaagaa ggagatgagg aagtattggc acaaaataca
    18361 gtagattttc tatcgtttag ttactatatg agcgtgacac aagcttatga ctttgaaaat
    18421 tatcaaagtg gtcagggaaa tatccttgga ggcctaacta atcctcattt aacaacttca
    18481 gaatggggat ggcaaatcga tcccatcggg ctacgcctag tcttaaatca gtattatgaa
    18541 cgttatcaga ttccgttgtt tattgtagaa aatggattgg gagcaaaaga tcaattgata
    18601 gaaacactag acggtgatta tactgtcgaa gatgattatc gtattgatta tatgaatcaa
    18661 catttagttc aagttgctaa agctattgaa gatggtgttg aaataatggg atatacatct
    18721 tgggggtgta ttgattgtgt atccatgtct acagctcaac taagtaaacg ttatggtcta
    18781 atatatgttg atcgaaatga tgatggaaca gggagtctac aacgttacaa gaagaagtca
    18841 tttggttggt accaaaaagt gattaagaca aatggccaat cattgtttga acatcataac
    18901 agataacatg ttaaacaact agtctctgtt tcaatttaat ataaaaagga gtatccatag
    18961 gttactcctt tttattataa tttatgaagg ttaatatctt ctgatttaag tgctagatac
    19021 aataaagttg catccttaaa gtttcttgga ttgagttgcg ttgattcata aatcttattt
    19081 aacctatatt gaacagtatt tttatggata aaaagttcct gactacattc ttttaatgat
    19141 agattatgct taaaatatga gttaagcaag atcttatcat tttcagataa gtttacaagc
    19201 gctttattta gcacatattg tttaatatta gaatcaatac ttgttaataa cagttcaaga
    19261 tcaaggtcgt cgacgaggtt gaccttttgt tgacctttta aagctttcag ggctaagata
    19321 gacgtttcat atgatctttt taaaagtgat aaagatgatt tttgtccgat accaacttta
    19381 tagaggccct tttcgcattc aaactttgat aggatatttg gataataata gtcaaacata
    19441 tggtcagaaa gtaaaagcca gtattgattg ggataattaa aggtataaat agcagtgtca
    19501 agtttatcaa aaaagttcaa gagtgacatt tcagattgac ttaagagtaa tttttgtttt
    19561 tgactattga tttcaaaagt gagtagacga tagtcagttt tttctgatag gtggtattgg
    19621 tttaaaaatt gattgaggta atagtagtct aactctctgc cttcaacaag atgatgtaag
    19681 acaatagaag cttcgttttt tcgcccgaaa tccagataat ctaattcatg ctcttttaag
    19741 atgagtcgag tcattttttg agcaagtaaa gcatattttc ctacttggtt ggggtttcca
    19801 ctaatcccaa tagtagctaa taattcacaa ttataataga aaggaatatt gatccctgct
    19861 tgtgtcccaa aataagattc ttgatctgtt acttcaatca tttgtcctgt ctgtgctact
    19921 tttaaaccaa tttcatggaa ttctccaaca cgttttggat tggtactagc gaaaatgatt
    19981 cctttggtat taataaaatt aatatcttgt tgacaaacat ctttgataga ggtaacgatt
    20041 tgttgtgcga gttgttttct taatttcatt cagcttacct tttgttatat ataacaaata
    20101 tcatatcata aaaacgctaa catttgttct gtagtctata ttttttgtta tttgattttg
    20161 ttactataat tgagtgatat aaaatagtga aaaaaataac aaagattcac aaaatatttt
    20221 caaggaggtt ttcaaatgaa ggtagtagta gctattgatt ctttgaaagg gagtctttct
    20281 tctttagaag ctggcaatgc tataaaggag tcaattaatg aagtaatctc aggggcggat
    20341 gtggaagtac atccattagc agacggggga gaaggaactg ttgaagcttt gacgctaggt
    20401 atggggggta cgatcgaaac aatccctgtt aaggggcctc ttggagaaaa agtacatgcg
    20461 agttatggta ttattccaca gcgtcagtta gctattattg aaatggctgc agctgctggt
    20521 attaccctaa ttgctactga agagagaaat cctctccaca caaccactta tggagttgga
    20581 gaaatgataa aggatgctat aagtaaaggc tgtaggcact ttattattgg tattggaggt
    20641 agtgctacta atgatggtgg ggcaggtatg ctacaagctt tgggatatgc attattagat
    20701 aaagataatc aagaaatcag tttgggagca caaggattgg cagatttaaa atctatctct
    20761 actgataagg ttattgagga attaaaagaa tgtgatttta aaattgcatg tgatgtaaca
    20821 aatcctttat gtggtgctca aggttgtagt tcgatttttg gaccgcaaaa aggtgctgat
    20881 gaagatatga ttacaaaaat ggatacttgg ttaagtaatt atgctacctt agctacttca
    20941 gttagtgaaa aagcagatgc aactattgaa ggaacaggtg cagcgggtgg gcttggattt
    21001 gcctttttag cctttacaaa tgctacctta gaacctggta ttgatattat tctatcagaa
    21061 attaatatag aaaaagcgat ttctgaagct gatttagtag ttactggtga aggtcgtttg
    21121 gatgggcaaa cggtgatggg aaaagctcca attggcgtag caaaattagc taaaaaatat
    21181 ggtaagaaag tagtggcatt ttctggatct gtcaccgaag atgctatact ttgtaatcaa
    21241 catggtattg atgctttttt tccaatcgta cgacgcctta tcagcttaga tgaggctatg
    21301 tctaaagagg tagcttataa aaatatgaaa gaaactgcta ctcaagtctt tagattaatc
    21361 aatttgtata atcattaata ttgaaaggaa ttttgataag ttatggaagc tacatttaca
    21421 acaacagggg ctttaatagg attagcgctt gctattttac tgattatcaa aaaagttcat
    21481 cctgcctata gtctcattct tggagcatta gttggtggtt tgattggtgg cggtgattta
    21541 gttacaattg tcaatacaat ggtattaggt gcgcaaggga tgatgtcatc catattacgc
    21601 attttaacat cagggatttt agcgggtgct ttaattaaaa ccggatcagc tgaaaaaatt
    21661 gcagaaagta tcattaaaaa attaggacaa cagcgagcca tcacagcttt agccatcgca
    21721 acaatgatta tttgtgcagt aggtgtcttt attgatattg cagttattac agttgctccg
    21781 attgcattag caataggtaa aaaagctaat ctttctaaat ctagcatact gttagcaatg
    21841 attggtggtg gtaaggctgg gaatattatc tcacccaatc ctaatacaat tgctgcttca
    21901 gaagcattta aggttgattt aacaagctta atggtacaaa atattattcc tgcaattgca
    21961 gctttagtag ttacgattat attagcaaag attgtttcca agaaaaataa cgatatttct
    22021 tatgatagtg aggagcaagt gggctctgat ctgccagcat tcttaccggc tatttctggt
    22081 ccacttgtgg ttatctgctt attagctctt cgtcctttat ttggaatcac tattgatccc
    22141 ttgattgcac tgccgttggg tggtctaatt agtattttag caacaggtta cctaaaagaa
    22201 acggtaccgt ttgttgaata cggtttatca aaagttgttg gcgtttcaat tcttttgatt
    22261 ggaacaggta cgctctcagg tattattaaa gcatctaatc tacaatttga tatgattcat
    22321 ctccttgaat ttttaaatat gccgaccttt atccttgccc cactctcagg tatttttatg
    22381 ggagcagcaa cagcatcaac gacttctgga acaactattg cttcacaaac gtttgctgaa
    22441 accttgatta aatcaggggt accggcggtt tcgggtgcag ctatgattca cgcaggtgcg
    22501 actgttttgg attcgctacc tcatggatcc ttcttccatg cgacaggcgg agctgtcaat
    22561 atggctataa aagatcgtat gaaactaatt agctatgaag ctttgattgg attaactagc
    22621 acaatagttg ctgtcgttta ctattgtttc tttgggtaaa atttggatga atacgtgatg
    22681 tttattatca ctgtttgaat agaattctca ttgatttttt ctaattttct tactataata
    22741 aattagtagg agaggtgttg tgaaacaaac aaagaaagaa tttattttag gattaaccat
    22801 tattattacc cttactttat taatcatgct gattttttta gtaatgttta ataagtccag
    22861 taactctaag ttgaagcaga caaaagatag aagaatatca tctacttcaa ccacaaggac
    22921 atccagtagc tcacatgatc aaaaaattgt agaaaagtct cttgaaaaaa agaaaatggt
    22981 catggaacaa gatatggaat tgatgaatgc ctataggctc tattatgatt atgcccattt
    23041 gtccttgaaa gaggttgttc tggcctatat gtctgaatat ggcattgaaa aaaatagtgt
    23101 tgctttttct tataaaaatt taaaaacagg acaaagggac gctatgaatg atactcaggc
    23161 tatgacagca gggagcacct ataagttacc tcttaacatg ttggtagtag atgcagtggt
    23221 caagggcaag ttgtccatgg aagaacgctt tgatattacc gagactgact atgaatatat
    23281 tggtgaacat aataattatg ttgctgcttt ctcgggggca atgtctattg atgatatgca
    23341 aaaatactct ttagtttatt cagaaaatac acccgcctat gctttagctg agcgtattgg
    23401 tggcatggat agtgcttatt caaagtttgg tcgttatggc caatctaaag gagacatcaa
    23461 aaatatacaa aaaaacggta ataaagtgac aacagattac tatattcaag tgctagacta
    23521 tctctggaaa catcgtaaaa agtatgatag tttaattact tatttagaag aagcttttcc
    23581 aacagattat tatcgtgctt tgataccatc tgatgttgtt gttgctcaaa agccagggta
    23641 tgtccgtgag gcgttgaatg ttggagcaat tgtcaaagaa gaagtgcctt atattgttgc
    23701 tatttatact gcaggtttag gtggctctac tcaggaagat tcagaaatta atggagtggg
    23761 actatatcag ctagaacaat tatgttttgt tattaatcaa tggcatcgtg ttaatatgaa
    23821 ttagaaataa atctatcaaa ataataaaaa cctcttagat taactatcta agaggttttt
    23881 ataaatgtga ttttataact tggttaagtg aagaaagggc agaaacagtt ttttgataag
    23941 attcaggact aatttctgag aaaaaatacc cgcacgattg caccgcattg acaacggcat
    24001 cttgtgactc aatacctttg ttacttagat gaataatgag ttgcctttta tcatctcttg
    24061 gtttttctcg tacaatccac ccacgatttt ccatacgctt taaaactgga gttagggtat
    24121 tcgaattaag agatagtgca taactaagtt ccttcatatg ccgatgatcc tcttcccaaa
    24181 ggactaacaa gataacgtat tggagataag tcaattgaaa ttcatccaaa gctttttggt
    24241 aaaatttatt gaaaagtcga tgtgtttcac taaccgtaaa acataatcgc tcgtctaaaa
    24301 tacttcgatt actcattatc atattgtagc attttggagg cgttatagta tgtcaatcgt
    24361 gttaaaaaat gataaaactt tttcctaatt gaaagcctat tcttacttaa caaacatagc
    24421 atcaccaaag ctgaagaaac gatatcgttc ttcaactgca tggttgtaag cttctagtac
    24481 aaaatcacgt ccagcaaatg ctgatactaa catgacaaga gtagactttg gtaagtgaaa
    24541 gttggttgaa aaagcgtcta caaccttgaa ctgataacca ggcttgataa aaatatttgt
    24601 ccaccctgag tcggctttta attcaccatt gaatttagaa ccgattgtct ctaatgtgcg
    24661 aatagaagtt gtaccaacag ctacaatacg tccaccagat tctttaactg cattgagagt
    24721 atcagcagct tctttagata attggtaaaa ctcagaatgc atttcgtgtt catcaagatt
    24781 atcaactgaa acaggacgga aagttccaag tccaacgtga agcgtcaaat agacaagctt
    24841 cacacctttt gtttcaattt tttccaatag ttcttttgta aaatggagac cggctgttgg
    24901 tgcagcagca gaaccatttt cttttgcata cacagtctga tagcggtcac gatcttctag
    24961 tttttcatga atatatggcg gaagtggcat ttcgcccaaa ctttcaagga cttctagaaa
    25021 aataccatca tatgagaatt ctacaatacg cccaccatgt tctaattcct ttgtgactgt
    25081 tgctattaac cgaccatcac caaatgaaac tttggtccct actcgtaagc gtttagcggg
    25141 tttagcaagc acttcccatt gatcgccttc tgtatttttg agaagtaata actctacatg
    25201 gccgtgtgta tcttgctttt caccataaag gcgagcgggc agaacacgtg tgttattcat
    25261 tactaaagca tcaccaggct tgagttcatc taagatatga tcgaaatggc tatctgtcat
    25321 cgtcttattc ttatgatcaa tgactaataa tttagaagcg tctctttttt caagtggtgt
    25381 ttgggcaatg agttcttctg gtaaataaaa atcaaaatcg tttgtattca tgatttctcc
    25441 tatctataag taagcttttc tattatatca cgttcagtaa aaatgaccca actaatcttt
    25501 ttatttagaa tgattacaaa aaataattgt aatcattcta aataagtgat ataataattg
    25561 tcagaaagtg agaagtagag aagatgaaag aagcaaaaaa tttttcccaa caattgaata
    25621 ttttaagagc tggtgttctt ggtgctaatg atggtatcat ctctgtagct ggtgttgtta
    25681 ttggtgttgc gagtgcaact cataaccttt ggattatttt tttatcagca gcttccgcga
    25741 ttttagctgg tgcattttct atggctggcg gggagtatgt tagtgtttct actcaaaaag
    25801 atactgaaca agcagcagtt gcgcgtgaag agaaactact agaaaataat ccagaacttg
    25861 ccaaaaaatc cttagttgat atctacttag caaaaggaga aagtcacgaa catgctcaat
    25921 ggctagttga caaggctttt tcaaagaatg ctattgagca tttagttgaa gaaaaatatg
    25981 gtattgagtt cggtgaatat acaagcccct ggcatgctgc tatatctagt tttatagctt
    26041 ttgccatcgg atcaattttt ccaactataa ctattctgtt attgccattt tcggtacgaa
    26101 ttgtaggaac ggtaataatt gttattgttt cactattatc aactggttat gtaagtgcta
    26161 agttaggtca agctcctaca gtacctgcca tgcgtcgaaa tgtgatgatt ggttgtttga
    26221 caatgttggc aacctacgtc attggacaat tattttcaat ataaaatatt aatcgtggag
    26281 ttattgtcta aactagaatg gacagtaatt ctttttagtt ttataacgaa attaagaagt
    26341 ttttattgac aaaaattggt atataccata taatataatc ataaaggtct ataccaaata
    26401 aaaggagagt aaacatgaga gttattacag ttaagaacga cattgagggg ggaaaaattg
    26461 catttactct tttagaagag aaaatgaaag ctggtgctca aactttagga ttggcaacag
    26521 gaagtagccc aatcaccttt tacgaagaga ttgtaaaaag taatcttgat ttttcaaata
    26581 tggtttctat taaccttgat gaatatgttg gaattgccgc aagtaatgat caaagttact
    26641 cttattttat gcataaacat ctttttgatg ctaaaccttt caaggaaaat aaccttccaa
    26701 acggcttggc taaagatttg aaagaagaaa taaaacgata cgatgctgtc ataaatgcta
    26761 atccaattga ttttcaaatc cttggaattg gtcgtaacgg tcatattggt tttaatgaac
    26821 caggtacacc ttttgatatt acaacacatg tggttgattt agctccaagt acaattgaag
    26881 ctaatagtcg tttctttaat agtattgatg atgtaccaaa gcaagcctta tcaatgggta
    26941 taggctctat tatgaaatca aaaacaatcg tattagtggc gtatggtatt gaaaaagcag
    27001 aagcgattgc aagtatgatt aaaggaccta tcacagaaga tatgccagca agtatcctcc
    27061 aaaaacatga tgatgttgtg attattgtgg atgaagcagc ggcatcaaag ttgtaaagta
    27121 aataataaaa aagtaaggat gaatctcaat gagaaaaaat tcattcttac ttttttatta
    27181 tgctttatta aattgtccag tctaagttag gtccagctgg aataatacca gtagggttaa
    27241 tggttttatg gctaccgtaa tagtgttttt tgatatgatc aaaattaact gtctcagcaa
    27301 ttccaggtag gttgtaaata cgttttgtat aatgccataa atgtgggtaa tcatgtagcg
    27361 cttttaaatt acatttaaaa tgaccataat aaacggtgtc aaagcgaact aaagttgtga
    27421 aaaggcgaat atcagcttcg gtaaattgtt ctccaacaag ataatggtta tcagataagt
    27481 gtttttctaa ctgatctaaa gctgtgaata aggtttcaac ttccttttga taaacgttct
    27541 gagaagtggc aaaaccagct ttatagacac cattatttat ttttggataa ataaaattat
    27601 tcatttcgtc aatttgtcct tgtaaggaat cgggatagta atcgtcagta tttccagtaa
    27661 tatgattaaa tgcagtattt aacatacgca taatttcaga tgattcattg ttaacgattg
    27721 tatggaattt tttatcccaa agaacaggga ctgtcacgcg tcctgtataa tcactttgac
    27781 ttgcttgata gatttggtaa aggtattggc tttggttaat catatcagga ataacgcctt
    27841 tatattcttg aaaagtccaa ccattctcca acattaaagg gttaacaatg gagatagaga
    27901 tatgagattc tagattttta agctttctca tgattaatac acgtgatgcc caagggcaag
    27961 ctagtgaaac gtagagatga tatcgtccag attctgcttt aaaacctgca tccccactag
    28021 gccctgctga accatcttta gtaacccaat gacggaattg tgtaacggtt cggacgaatt
    28081 taccaccagt tgatgcagta tcataccatt gatcaaccca tttgccatct actaaaagtc
    28141 ccatgataat ctcctttgtt tttctattac taattagtag tataaagatt ttaaaaagaa
    28201 ttagcaaatg tttaacttac agtatggtaa actaagggta tgcgtttaga taaattatta
    28261 ggtcaggcag gatttgggtc tagaaatcag gttaagaaat taatttgttc taggcaagtt
    28321 tctgtagatg gtcaaattgt tacaaaggat aatgttattg tagatagtgg tctacaatcg
    28381 atttttgtag gaaaagagag ggtctgccta aaagaaagtt cttattacct tctttataag
    28441 ccatcaggag ttgtttcagc agttagagat tcagaacata aaacagtcat agacttaatt
    28501 agcgaaaaag ataaggtgga gggattgtat ccgattggtc gtctagatcg tgatacggaa
    28561 gggcttttaa tagtcacgaa taatggtcca ctgggttacc gaatgttaca tccaaaacac
    28621 cacgttgcaa aaacatatta tgttgaagta aatggtttct tggaaagaga tgctattacc
    28681 ttttttgaag aaggagttgt atttgacgat ggaacaaagt gtaaaccagc tgaattaacg
    28741 attgatacgg ctaataatga caaaagtacc gcaagaataa ctatcacaga agggaaattt
    28801 caccaagtaa aaaagatgtt tcttgcatat ggcgttaaag ttatatactt gaggcgtatt
    28861 tcttttggtg acttgaggct tgatatgaat ttgaaaccag gtcagtatcg tagattgaga
    28921 gattcagagg cagctatctt aaaaaggtat ttagattagt gaaacgactc acttgacctt
    28981 gcttagtatt cttcatatac ttataagtga ttattttaaa ggagaaaacc attgaattca
    29041 aaactttcat tgaaattgac tattttatcg atttctatct ttttgatgtc tcatttagca
    29101 attgctcctg caattcctaa attgtatgcc ttttaccata ctcaaaaccc tcatattgga
    29161 ctagcttcag tagagagttt agttactatt ccagctatga tgattactat ttttgttatt
    29221 ttgagtaatt ttgtagtcac aaaacttggc aaaaaaaaca cggtcttact tggtttatgt
    29281 ttaatcttaa tgtctgggtt tatctcattt tttacaagta atttttcact tgcgatggct
    29341 agtcgactac ttttgggtat tggtattggt ctctataatt cgctatcaat tagtatcata
    29401 acggatttat atgaggctga tgagagagcc agtatgattg gtcttagaac agcatcactc
    29461 aatatcggta aagccttaac tacatttatt gttggattgg ttttagctat tggtgtgaat
    29521 tatatttatt tggtttacct tctggttatt cctgttttct ttttcttttg gaaaaatgtt
    29581 ccagaggttg aaaatcaaac acataccctt aaagctagta caacatttga tactaaagcg
    29641 gctttgttga tgctcatcac gttcttagta ggtattgctt atataggagc aactgtcaaa
    29701 attccaacct tactagtgac aaaatatcac tacgcgacga gcttctctag taatatgtta
    29761 actctccttg cttttagtgg tattttagtt ggatctgtat ttggaaaatt agttaaagta
    29821 ttccaagaga agacgctatt aatcatgatt ctagctatgg gaataggtaa tgtgctattt
    29881 gctttagcta ataatcagat catttttatc gtagctagta ttttaatagg agctagtttt
    29941 gttggcacca tgtcttctgt attcttttat atttcaaaaa actatgcaaa agaacataat
    30001 aactttatca caagccttgc cttaacagct ggtaatatag gagttatttt gacaccgctt
    30061 attttaacga aacttccaag ccagctacat ctggaacctt ttatgacacc atttttaata
    30121 acaagtgggc tgatggtgat taatgtcttt gtttacctag ttttaatgtc aaaaaataaa
    30181 taaaaagtta tccgaaagga taactttttt cgaatagtaa aagtaggaga aaaaatgtta
    30241 attgctaaag acaaacaagg aaatttaatt aatctactcg agtctcatcc gggaaaggga
    30301 cagtattttt gtccaacttg ttgttcagct gtaaggttaa aggcaggtcg aatcatgaga
    30361 agacactttg cacatattag tctaaaaaat tgtcagtttt atcatgaaaa tgagtctaat
    30421 gagcacttgc aattaaaagc aaagttatat atgagtttaa gtagagagaa tgaaactatg
    30481 ctcgagcatc atttgccaga aattaatcag attgcagatt tatttgtgaa tgagacattg
    30541 gctttagaag ttcagtgtag ccgactatca gagcagcgac tcagggagcg aacaaaagca
    30601 tatctacagg cagactttca agtgagatgg ttactagggg aaaaactctg gttaaagcat
    30661 cgtttaacaa atctacataa acaattttta caattttctc aaagtatcgg atttcatatt
    30721 tgggagttag atttgaggtt agaagtactg agactaaaat atttgattta cgaagattta
    30781 cgcggacatg tctattactt aagtaaaact tgccctctta gtggagacgt
//
LOCUS       DATRMA010000016        30459 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.016, whole genome shotgun sequence.
ACCESSION   DATRMA010000016 DATRMA010000000
VERSION     DATRMA010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 30459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30459
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.016"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            778..1260
                     /locus_tag="VBC28_000705"
     CDS             778..1260
                     /locus_tag="VBC28_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6814706.1"
                     /translation="MKNEYEEILKKDNLFEHEHAEFKESLNLNESFYLQLKKEEMKPS
                     DNLKKEEKPRENSVKERETPAENNDFVSVTEKNNLIDKYKKLLSKIPENTQNPGEKNI
                     RNYLEKEYEELLQKDKLFKHEYTEFTKSLNLNETFYSQLKEGEMKLSENPEKGETNTN
                     "
     gene            complement(1431..2045)
                     /locus_tag="VBC28_000706"
     CDS             complement(1431..2045)
                     /locus_tag="VBC28_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEO6814707.1"
                     /translation="MSFLEELKNRRSIYALGRNTEVSDEKIVEIIKEAVRQSPSAFNS
                     QTSRVVILLNDEVTKFWDELVANDLVETMKVQGAPETAIAGTKEKLASFGASKGTVLF
                     FEDQDVVKSLQEQFVLYADNFPVWSEQSTGIASVNTWTALSAELGLGGNLQHYNPVID
                     ASVQAVYGVPASWKLRGQLNFGSIEAETGEKEFMNDDDRFKVIG"
     gene            complement(2178..2603)
                     /locus_tag="VBC28_000707"
     CDS             complement(2178..2603)
                     /locus_tag="VBC28_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEO6814708.1"
                     /translation="MGNFKNSAVKSMVVMRKAFRTIDGKVSESFKEFELTPTQFAVLD
                     VLYAKGTMKIGELIENMLATSGNMTVVIKNMEKKGWVLRHSCPNDKRAFLVSLTTEGE
                     EVIKKALPEHIKRVEDAFSVLTETEQEDLINLLKKFKTL"
     gene            complement(2730..7136)
                     /locus_tag="VBC28_000708"
     CDS             complement(2730..7136)
                     /locus_tag="VBC28_000708"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HEO6814709.1"
                     /translation="MSELFKKLMDQIEMPLEIKNSSVFSSADIIEVKVHSLSRLWEFH
                     FSFPELLPIEVYRELQTRLVNSFEKADIKATFDIRAETIDFSDDLLQDYYQQAFCEPL
                     CNSASFKSSFSQLKVHYNGSQMIISAPQFVNNNHFRQNHLPRLEQQFSLFGFGKLAID
                     MVSDEQMTQDLKSSFETNREQLLEKANQEAMQALEAQKSLEDSAPPSEEVTPTQNYDF
                     KERIKQRQAGFEKAEITPMIEVTTEENRIVFEGMVFSVERKTTRTGRHIINFKMTDYT
                     SSFAMQKWAKDDEELKKYDMISKGSWLRVRGNIENNNFTKSLTMNVQDIKEIVHHERK
                     DLMPADQKRVEFHAHTNMSTMDALPTVESLIDTAAKWGHPAIAITDHANVQSFPHGYH
                     RAKKAGIKAIFGLEANIVEDKVPISYNEVDMNLHEATYVVFDVETTGLSAANNDLIQI
                     AASKMFKGNIIEQFDEFIDPGHPLSAFTTELTGITDNHVRGSKPILQVLQEFQNFCQG
                     TVLVAHNATFDVGFMNANYERHNLPLITQPVIDTLEFARNLYPEYKRHGLGPLTKRFQ
                     VALEHHHMANYDAEATGRLLFIFLKEARENRDVTNLMELNTKLVAEDSYKKARIKHAT
                     IYVQNQVGLKNIFKLVSLSNVKYFEGVARIPRSVLDAHREGLLLGTACSDGEVFDALL
                     SNGIDAAVTLAKYYDFIEVMPPAIYRPLVVRGLIKDEVGIQQIIRDLIEVGRRLDKPV
                     LATGNVHYIEPEDEIYREIIVRSLGQGAMINRTIGRGEDAQPAPLPKAHFRTTNEMLD
                     EFAFLGKDLAYEIVVTNTNTFADRFEDVEVVKGDLYTPFVDRAEERVAELTYAKAFEI
                     YGNPLPDIIDLRIEKELASILGNGFAVIYLASQMLVQRSNERGYLVGSRGSVGSSFVA
                     TMIGITEVNPMPPHYVCPNCQHSEFITDGSCGSGYDLPNKNCPKCGTLYKKDGQDIPF
                     ETFLGFDGDKVPDIDLNFSGDDQPSAHLDVRDIFGEEYAFRAGTVGTVAEKTAFGFVK
                     GYERDYNKFYNDAEVERLATGAAGVKRSTGQHPGGIVVIPNYMDVYDFTPVQYPADDM
                     TAAWQTTHFNFHDIDENVLKLDILGHDDPTMIRKLQDLSGIDPSNILPDDPDVMKLFS
                     GTEVLGVTEEQIGTPTGMLGIPEFGTNFVRGMVNETHPTTFAELLQLSGLSHGTDVWL
                     GNAQDLIKEGIATLSTVIGCRDDIMVYLMHAGLQPKMAFTIMERVRKGLWLKISEDER
                     NGYIQAMRDNNVPDWYIESCGKIKYMFPKAHAAAYVLMALRVAYFKVHYPIFYYCAYF
                     SIRAKAFELRTMSAGLDAVKARMKDITEKRQRNEATNVENDLFTTLELVNEMLERGFK
                     FGKLDLYRSHATDFIIEEDTLIPPFVAMEGLGENVAKQIVRAREDGEFLSKTELRKRG
                     GVSSTLVEKFDEMGILGNLPEDNQLSLFDDFF"
     gene            complement(7259..7843)
                     /locus_tag="VBC28_000709"
     CDS             complement(7259..7843)
                     /locus_tag="VBC28_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HEO6814710.1"
                     /translation="MKSRKKDKLVLRLTTTLLVFGLGGVWFYNYKNDNVEPTVTSASD
                     QTTTFIQTISPTAIEISKTYDLYASVLLAQAILESSSGQSDLSKAPNYNLFGIKGEYK
                     GKSVQMPTLEDDGKGNMTQIQAPFRAYPNYSASLYDYAELVSSQKYASVWKSNTSSYK
                     DATAALTGLYATDTAYASKLNQIIETYSLDAYDK"
     gene            complement(7968..9821)
                     /locus_tag="VBC28_000710"
     CDS             complement(7968..9821)
                     /locus_tag="VBC28_000710"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEO6814711.1"
                     /translation="MKQSKMLIPTLREMPSDAQVISHALMVRAGYVRQVSAGIYAYLP
                     LANRTIEKFKTIMRQEFEKIGAVEMLAPALLTADLWRESGRYETYGEDLYKLKNRDQS
                     DFILGPTHEETFTTLVRDAVKSYKQLPLNLYQIQSKYRDEKRPRNGLLRTREFIMKDG
                     YSFHKDYEDLDVTYEDYRKAYEAIFTRAGLDFKGIIGDGGAMGGKDSQEFMAVTPNRT
                     DLNRWLVLDKTIPSIDDIPEDVLEEIKVELSAWLVSGEDTIAYSTESSYAANLEMATN
                     EYKPSTKAATFEEVTKVETPNCKSIDEVAGFLSIDENQTIKTLLFIADEQPVVALLVG
                     NDQVNDVKLKNYLAADFLEPASEEQAKEIFGAGFGSLGPVNLPDSVKIIADRKVQDLA
                     NAVSGANQDGYHFTGVNPERDFTAEYVDIREVKEGEISPDGKGTLKFARGIEIGHIFK
                     LGTRYSDSMGANILDENGRSNPIVMGCYGIGVSRILSAVIEQHARLFVNKTPKGAYRF
                     AWGINFPEELAPFDVHLITVNVKDQESQDLTEKIEADLMLKGYEVLTDDRNERVGSKF
                     SDSDLIGLPIRVTVGKKASEGIVEVKIKASGDTIEVHADNLIETLEILTKK"
     gene            complement(9913..11172)
                     /gene="rseP"
                     /locus_tag="VBC28_000711"
     CDS             complement(9913..11172)
                     /gene="rseP"
                     /locus_tag="VBC28_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEO6814712.1"
                     /translation="MLGILTFIIIFGVIVVVHEFGHFYFAKKSGILVREFAIGMGPKI
                     FSHIDKEGTTYTIRILPLGGYVRMAGWGDDKTEIKTGTPASLTLNKEGIVTRINLSGK
                     QLDNTSLPINVTAYDLEDKLTITGLVLSETKTYSVDHDATIIEEDGTEIRIAPLDMQY
                     QNASVWGRLITNFAGPMNNFILGLVVFIALAFIQGGVQDLSTNQVRVSENGPAASAGL
                     KNNDRILQIGSHKVSNWEQLTAAVEKSTRHLEKKQKLALKIKSKEVVKTINVKPQKVD
                     KSYIIGIMPALKTSFKDKLLGGLKLAWESFFRILNELKKLIAHFSINKLGGPVALYQA
                     SSQAAKNGFVTVLNLMGLISINLGIMNLIPIPALDGGKIVMNILEAIRRKPLKQETET
                     YITLAGVAVMLVLMIAVTWNDIMRAFF"
     gene            complement(11203..11997)
                     /locus_tag="VBC28_000712"
     CDS             complement(11203..11997)
                     /locus_tag="VBC28_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002275264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEO6814713.1"
                     /translation="MKERVIWGAVALAIFIPFLVMGGLPFQFLVGLLAMIGVSELLRM
                     RRLEIFSFEGALAMIGAFVLTVPLDSYLSFLPVDASLSAYGIVIFMILAGTVLNSNSY
                     SFEDAAFPIASSFYVGIGFQNLVSARMAGIDKVLLALFIVWATDIGAYMIGRQFGQRK
                     LLPSVSPNKTIEGSLGGIASAIVVAFFFMLFDKTVYAPHSFLVMLVLVAIFSIFGQFG
                     DLVESSIKRHFGVKDSGKLIPGHGGILDRFDSMIFVFPIMHFFGLF"
     gene            complement(12012..12764)
                     /locus_tag="VBC28_000713"
     CDS             complement(12012..12764)
                     /locus_tag="VBC28_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEO6814714.1"
                     /translation="MFIFKRKKQVEMPLEKIPAHIGIIMDGNGRWAKKRLKPRVMGHK
                     AGMDALQEVTIAASGLGVKVLTVYAFSTENWSRPDDEVKFIMNLPVEFFDKYVPELDK
                     NNVRVQVIGDTHKLPKATYDAMQRACLRTKHNSGLVLNFALNYGGRSEITNAIKEIAQ
                     DVLEAKLNPDDITEDLVANHLMTNSLPYLYRDPDLIIRTSGELRLSNFLPWQSAYSEF
                     YFTPVLWPDFKKDELHKAIVDYNQRHRRFGSV"
     gene            complement(12931..13272)
                     /gene="yajC"
                     /locus_tag="VBC28_000714"
     CDS             complement(12931..13272)
                     /gene="yajC"
                     /locus_tag="VBC28_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000056605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEO6814715.1"
                     /translation="MSLPIIMLVVMVGMMFFMQRQQKKQAQERQKQLNAVQKGDEIVT
                     IGGLFGVVDEVNTEAQRMVLDVDGVYLTFELAAIKSVVSKAATPTEPVEDVKIIEVNE
                     DENKETAIETE"
     gene            complement(13349..13693)
                     /locus_tag="VBC28_000715"
     CDS             complement(13349..13693)
                     /locus_tag="VBC28_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductase thioredoxin"
                     /protein_id="HEO6814716.1"
                     /translation="MYSFEELLATMTLITAAEIEDKIDSNQDFVLFIGRISCPFCHLF
                     VPKIVEVADEDEFELFHLDSEDFDHWTANKEFRNKYDIPTVPGLMVVKNGTIKVKCDS
                     KMTKEEIREFIG"
     gene            complement(13884..15047)
                     /locus_tag="VBC28_000716"
     CDS             complement(13884..15047)
                     /locus_tag="VBC28_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HEO6814717.1"
                     /translation="MSENLGQLAINQARENGGKLEVISKVKVEDKRDLSIAYTPGVAS
                     VSSAIAEDVELAYELTTKKNTVAVVSDGSAVLGLGDIGPEAAMPVMEGKAALFKRFAN
                     VDAVPIVLKTNDTEEIISIVKAISPTFGGINLEDISAPRCFEIEQRLIEECDIPVFHD
                     DQHGTAIVVLAALFNSLKLVKKDIEDIRVVVNGGGSAGLSITRKLLSAGAKHVTVVDR
                     FGIINDKDRESLAPHHKAIAKLTNREFQSGSLEDALENADVFIGVSAPEALHAEWISK
                     MADKPIVFAMANPIPEIYPDQALKAGAYIVGTGRSDFPNQINNVLAFPGIFRGALDAR
                     AKTITVEMQIAAARGIASLIPEEELSTTHIIPNAFQNDVADVVAKSVSNAVQK"
     gene            complement(15072..16409)
                     /locus_tag="VBC28_000717"
     CDS             complement(15072..16409)
                     /locus_tag="VBC28_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000786046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HEO6814718.1"
                     /translation="MADVKVVNNEDSRGQKQDLKAKLFHIKIGSVPLPVYVCLALLIL
                     LAGFLQKLPVNMLGGFAVILTMGWFLGTIGASIPGFKNFGGPAILSLLVPSILVFFNL
                     INKNVLESTNMLMKQANFLYFYIACLVSGSILGMNRKMLIQGLLRMIFPMLLGMVCAM
                     MVGTFVGVILGLEWRHTLFYIVTPVLAGGIGEGILPLSLGYSSITGVASEQLVAQLIP
                     ATIIGNFFAILCTALLNRLGEKKPHLSGQGQLVRLNKGEDMSDIIADHSGPIDVKKMG
                     GGVLTACSLFIFGHLLQQLTGFPGPVLMIVAAAILKYINVIPRETQNGAKQLYKFISG
                     NFTFPLMAGLGLLYIPLKDVVATLSIQYFIVVISVVFTVISVGFFVSRFLNMNPVEAG
                     IISACQSGMGGTGDVAILSTADRMNLMPFAQVATRLGGAITVITMTAILRMLF"
     gene            16563..18089
                     /locus_tag="VBC28_000718"
     CDS             16563..18089
                     /locus_tag="VBC28_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEO6814719.1"
                     /translation="MKKKLSLWAFLSLILVTMTICIFSIFYYVTIHQSYRMVRVQEEK
                     ILKNTGYALSRNPQVIQTLKDNHYDQSLQKQMLFLSKKSNLDYIVLINLKGIRFTHPD
                     STKIGKPFQGGDEQAVFKGKAIMSTAEGSLGKSLRYLIPVYDHQKQVGAIAVGLKLTT
                     LGDLSQSSIKEFSKPLLISILISLVVTSIISYGLKKQLHNLHPSDIFQHLEERNATLD
                     QIQAAVFVIDQRHIIKRNNPAASLLFKKEGQRDLFSGKLLESLIPQLKQDHFSKKTEQ
                     VLHFQGQDYLLSISPITVKTQNRGYVVFLRNVTETLFTLDQLAHTTAYASALQAQTHQ
                     FMNQLHVIYGLTDIEYYDELKIYLKELLEPQNEFLARLSMLVREPRLASFIIGEREKF
                     AEKHINLSTEILVEIPTKSTVEDVNNYLLLHRYINTKILTLLNSTTLVSLRLNYQNNL
                     IETDYQWENEKWLLNDYHQYFNDAYFQQLLVDSRATYKISLKQNKVIIRTNIIYSEGP
                     "
     gene            18091..18780
                     /locus_tag="VBC28_000719"
     CDS             18091..18780
                     /locus_tag="VBC28_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEO6814720.1"
                     /translation="MDVLIIEDDPMVEFIHRNYLEKLNYFQNIYSTASQTQAIAYLND
                     IKIQLVLLDIHIKEGNGLELLKLLRNQHQNTEVIVISAANEAHTVKEAFHLGIVDYLI
                     KPFTFERFESSIEKFLNHYHTFEADKIYQDNIDHFQKIDSGWLEGEVKLDEKGLSEIT
                     YQHILDAIQELEQPFTIQELAKCSQFSHVSVRKYIAYMEEKGLLTSQQIYTKVGRPYK
                     VYKLIKKNLGL"
     gene            complement(18793..19788)
                     /gene="galE"
                     /locus_tag="VBC28_000720"
     CDS             complement(18793..19788)
                     /gene="galE"
                     /locus_tag="VBC28_000720"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000279623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEO6814721.1"
                     /translation="MAVLILGGAGYIGSHMVDQLITQGKEKVIVVDNLVTGHRQAVHS
                     DAIFYEGDLSDKTFMRQVFRENPDVDAVIHFAAFSLVAESMENPLKYFDNNTAGMIKL
                     LEVMNECDIKNIVFSSTAATYGIPEQVPILETAPQNPINPYGESKLMMETIMKWADQA
                     YGIKFVALRYFNVAGDKPDGSIGEDHKPETHLLPIILQVAQGVRDKIMIFGDDYNTPD
                     GTNVRDYVHPFDLADAHILAVDYLRQGNESNVFNLGSSTGFSNLQMLEAARRITGKEI
                     PAQKAARRPGDPDTLIASSEKARQILGWEPKFDNIDKIISSAWAWHSSHPNGYED"
     gene            complement(19876..21483)
                     /locus_tag="VBC28_000721"
     CDS             complement(19876..21483)
                     /locus_tag="VBC28_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEO6814722.1"
                     /translation="MKKHWWHKATIYQIYPRSFMDSDGDGVGDIKGITSKLDYLEKLG
                     ITAIWLSPVYQSPMDDNGYDISDYQAIADIFGDMNDMDQLLQEANQRGIKIIMDLVVN
                     HTSDEHAWFVEARENPNSPERDFYIWRDEPNDLTSIFSGSAWEYDKVSGQYYLHLFSK
                     RQPDLNWENEALRHKIYDMMNFWIDKGIGGFRMDVIDLIGKIPDKGITGNGPKLHDYL
                     KEMNRASFGKHDLLTVGETWGATPDIAKQYSNPDNEELSMVFQFEHVGLQHKPDAPKW
                     DYSDGLDVPALKDIFTKWQTQLELGQGWNSLFWNNHDLPRVLSIWGNDSDNRKQSAKA
                     LAILLHLMRGTPYIYQGEEIGMTNYPFECLADVDDIESLNYAKEAMDNGVSEATILDS
                     IRKVGRDNARTPMQWSQEHQAGFTKGTPWLAVNPNYQEINVEAALNDTESIFYTYQKL
                     VALRKEHDWLVDADFKLLETADKVFAYVRQTDKERYLIVANLSDQNQSFEFPEAVKET
                     IISNTEVQEVLSSNTLKPWDAFCIELF"
     gene            complement(21611..22744)
                     /gene="ugpC"
                     /locus_tag="VBC28_000722"
     CDS             complement(21611..22744)
                     /gene="ugpC"
                     /locus_tag="VBC28_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEO6814723.1"
                     /translation="MVELNLNHIYKKYPSASHYSVEDFDLDIKDKEFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELKIDGEVVNDKSPKDRDIAMVFQNYALYPHMTVYDNMAFGL
                     KLRKFSKQEIDKRVREAAANIGLTEFLERKPADLSGGQRQRVAMGRAIVRDAKVFLMD
                     EPLSNLDAKLRVSMRAEIAKIHQRIGSTTIYVTHDQTEAMTLADRIVIMSATKNPDGD
                     GTIGKIEQVGSPQELYNLPANKFVAGFIGSPSMNFFKVKVENGMIISEDGLRIAIPEG
                     QEKLLESRGYKGKELIFGIRPEDISSNLLVQDTYPNANVEAEVLVSELLGSETMLYVK
                     LGQTEFASRVEARDFHNPGEKVNLTFNVAKGHFFDADTEQAIR"
     gene            complement(22845..23696)
                     /locus_tag="VBC28_000723"
     CDS             complement(22845..23696)
                     /locus_tag="VBC28_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000347002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO6814724.1"
                     /translation="MDFKELFPEVITKQEVKQSEDYIIVEQDGHVLHFPKSSLTKREL
                     YLLQMTPSLEDASSVDSQNPWYRYLVEGRGRLPQSHSAVQFIFIEHQFTLSEELKDFL
                     SPLVINVETIMTINQTQSVMILNQDNFFNATELLTDILPTIENDFNTRLRCYFGNSWT
                     HLQAVDWKELYEEEYKLFTLFLSHKAEQHYCRFPKMALWALANQSPMPSIKAKCLQHI
                     LDTSDTSAIIKALWQEQGNLAKTAKALFIHRNSLQYKLDKFTQSSGLNLKILDDLAYA
                     YLISMDT"
     gene            complement(23791..24687)
                     /locus_tag="VBC28_000724"
     CDS             complement(23791..24687)
                     /locus_tag="VBC28_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HEO6814725.1"
                     /translation="MAITIQNHELQVTLKALGATMTSITDSQGVEYLWQGDATYWGGQ
                     APILFPICGSVRNDCVIYRPAQAPHFTGIIPRHGFVRHKTFDYDYISDSSVRFTIKSS
                     KEMLINYPYRFSLEITYTLRNKSIAITYIVKNLESEKNMPYAIGAHPGFNCPLFEKEV
                     FSDYYLEFEQFETCTIPESFPDTGLLDLQARHPFLENQKQLSLNHALFEKDAITLDQL
                     RSKTVYLKSRNHAKGIQLDFDDFENLILWTSNNGGPFLALEPWSSLSTSIEESDILED
                     KQNIVRLNPKQSKQHSIRITIL"
     gene            complement(24741..25718)
                     /gene="lacD"
                     /locus_tag="VBC28_000725"
     CDS             complement(24741..25718)
                     /gene="lacD"
                     /locus_tag="VBC28_000725"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000524687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HEO6814726.1"
                     /translation="MGLTEQKQKHMEQLSDKNGIISALAFDQRGALKRLMAKYQSEEP
                     TVSQIEALKVLVAEELTPYASSMLLDPEYGLPATKVLDDNAGLLLAYEKTGYDTSSTK
                     RLPDCLDIWSAKRIKEEGADAVKFLLYYDVDSSDEVNEEKEAYIERIGSECVAEDIPF
                     FLEILSYDEKITDSSGIEYAKIKPRKVIEAMKVFSNPRFNIDVLKVEVPVNMDYVEGF
                     AQGETAYNKATAAAYFREQDQATLLPYIFLSAGVPAQLFQETLVFAKEAGAKFNGVLC
                     GRATWAGSVKEYVEKGEAGARQWLRTIGFQNIDELNKILQKTATSWKER"
     gene            complement(25720..26652)
                     /locus_tag="VBC28_000726"
     CDS             complement(25720..26652)
                     /locus_tag="VBC28_000726"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HEO6814727.1"
                     /translation="MILTVTLNPSIDISYCLENFNMDTVNRVTDVSKTPGGKGLNVTR
                     VLSQLGDNVVATGLLGGDFGDFIRSGLDALEIRHQFLSIGGETRHCIAVLHEGQQTEI
                     LEKGPHITKDEADAFLNHLKLIFDAATIITVSGSLPKGLPSDYYARLISLANHFNKKV
                     VLDCSGEALRSVLKSSAKPTVIKPNLEELTQLIGKPISYSLDELKSTLQQDLFRGIDW
                     VIVSLGARGAFAKHGNHYYQVTIPKIEVINPVGSGDATVAGIASALEHQLDDTNLLKR
                     ANVLGMLNAQETLTGHINLTYYQELISQIQVKEV"
     gene            complement(26663..27178)
                     /gene="lacB"
                     /locus_tag="VBC28_000727"
     CDS             complement(26663..27178)
                     /gene="lacB"
                     /locus_tag="VBC28_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HEO6814728.1"
                     /translation="MKIAVGCDHIVTYDKIAVVDYLKTKGYEVIDCGTYDNIRTHYPI
                     YGKKVGEAVASGKADLGVCICGTGVGINNAVNKVPGIRSALVRDLTSAIYAKEELNAN
                     VIGFGGKITGGLLMTDIIEAFIRAKYKPTKENKVLIEKIAEVETHNAHQEENDFFTEF
                     LDKWNRGEYHD"
     gene            complement(27199..27624)
                     /gene="lacA"
                     /locus_tag="VBC28_000728"
     CDS             complement(27199..27624)
                     /gene="lacA"
                     /locus_tag="VBC28_000728"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HEO6814729.1"
                     /translation="MTIIIGADAHGVELKEVIRQHLTSLGKEIIDLTDTSKDFVDNTL
                     AIVAKVNQKEDNLGIMVDAYGVGPFMVATKVKGMIAAEVSDERSAYMTRAHNNARMIT
                     LGSEIVGPGVAKHIVEGFVDGTYDAGRHQIRVDMLNKMC"
     gene            complement(27872..30322)
                     /locus_tag="VBC28_000729"
     CDS             complement(27872..30322)
                     /locus_tag="VBC28_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HEO6814730.1"
                     /translation="MTLTFGKPSITIRRLGVITSTYMMLGFILLCGFLHPSTIYSEEI
                     KSDINSFISIDDEKTTVPIFDSTLDKLTNSIEERQLELDSDTSDSLIVDDQKDKDMVI
                     VLGDSDSQEKLSPPLKSEIATEENRKQANLAELNATQPNNRTTYIIPESSHSIAEQQR
                     FLIESKGYSVALLNSDEFRKTAREDRGFERDKLRSLDIIPKGDLSTSNVIGNTDIASQ
                     ISLGFKKNAIQEHHLTKTFSQKDGKLSSVIEGMLAIGKEKVEKEIKYSGNLWQKLKAK
                     AHCLVCCVDNLNFEDIKSYFQYYCHLNHQLKLPKGAILSAKTEVYRGGDFGRKNKDNV
                     FGYRIPSLLKTEKGTLLVGADERIEQACDWGNIGMVIRRSEDDGVTWGKRETIVNLRN
                     NPRVPLVTSGDYSGSPINMDMALVQDTSSKTKRIFSIYDMFPEGRGVISIANTPEKEY
                     TQIGGQSYLNLYNNGKKSKVFTIRDKGIVYNFKGKKTDYHVITETTKSDHSNLGDIYK
                     GKQLLGNIYFTKHKTSPFRLAKSSYVWMSYSDDDGRTWSSPRDITASLRQKGMKFLGI
                     GPGKGIVLKWGPHAGRIIIPAYSTNWKSHLRGSQSSRLIYSDDHGKTWHTGKAVNDNR
                     ILSNGEKIHSLTMDNKKEQNTESVPVQLKNGDIKLFMRNLTGNLEVATSKDGGETWQN
                     HVKRYKEVHDAYVQLSAIRFEHDKKEYILLVNANGPGKKRQDGYARLAQVNRNGSFKW
                     LYHHHIQDGSFAYNSVQQLNNDKFGVLYEHREKHQNSFTLNYKVFNWSFLSQNTEKQG
                     TLWEKMAANWHVLFKFYL"
BASE COUNT         9805 a         6124 c         4744 g         9786 t
ORIGIN      
        1 aagtttgcct ttgaaaataa ttcaaaagat tcttcatatt cttcaaacat taatattagt
       61 gaagataaaa taaaagaaaa tgaccaaaaa attctagatt taatagttaa atctggagct
      121 aataatcaaa acttaacaga tgaagaaaaa gtaattgctt tcactaaata tataggtgaa
      181 ataactaatt atgacaatga agcttacaga gctagaaatg tcgacacaga atattacaga
      241 gcttcagatt tattttctgt aacagaaaga aaacttgcta tgtgcgttgg ctatagtgta
      301 acagccgcta gggcatttaa tattatgggg atacctagtt atgtcgtttc tggtaaaagt
      361 cctcaaggaa tatctcatgc agctgtgaga gcgtattata atagaagttg gcatatcata
      421 gatatcacag ccagtacata ttggaaaaat ggtaattaca aaacaacgta ttccgatttt
      481 attaaagaat actgtataga tggttatgac gtttatgatc ccgcaaaaac aaataataga
      541 tttaaagtta agtatatgga atcaaatgaa gcgtttgaaa attggataca taataatgga
      601 agcaaatcta tgttatttat taatgaatct gcagctctaa aagataaaaa accaaaagat
      661 gattttgtac cagtaacaga aaaagaaaaa aacgagctga tagataagta taaaaaatta
      721 ttgtcacaga ttcctgaaaa cacacaaaat ccaggagaaa aaaacataag agattatttg
      781 aaaaatgaat atgaagaaat attaaagaaa gataatttat ttgagcatga acatgcagaa
      841 tttaaagaaa gtctaaattt aaatgaatca ttctacctac aattaaagaa agaagaaatg
      901 aaaccgagtg ataatctcaa aaaagaagaa aaaccaagag aaaattcagt aaaagagagg
      961 gagactccag ctgaaaacaa tgattttgtt tcagtaacag aaaaaaacaa cttgattgat
     1021 aaatataaaa aattattatc aaaaatccca gagaatacac aaaatcctgg ggaaaagaat
     1081 atacgaaatt atctagagaa agaatatgaa gaactactac aaaaagataa attattcaag
     1141 catgagtaca cagagtttac aaaaagtcta aatttgaatg aaacattcta ttcacaatta
     1201 aaagaaggag aaatgaaact aagtgaaaat cccgaaaaag gagaaacgaa cacgaattaa
     1261 cctttcaaat atctacatca aataacagaa gtctctgtat caagcgaatt gtagcttttt
     1321 tgaatatact aaaaacactc ctacctctat tttactagaa gtaggagtgt tttttcattt
     1381 tagttcatag atgcagtgtc attgctatcc ttgccttttt ttgtacagta ttatccgatg
     1441 actttaaagc ggtcatcatc attcatgaat tctttttctc ctgtttcagc ttcgattgaa
     1501 ccaaaattta gttgtccacg aagtttccaa ctagctggaa caccataaac agcttgtact
     1561 gatgcatcaa taactggatt gtaatgttgt aaattaccac caagtccaag ttctgcagaa
     1621 agggctgtcc aagtatttac tgaggcaata cctgttgatt gctcagacca aactggaaag
     1681 ttatccgcat aaagaacgaa ttgttcttga agagatttga caacatcttg atcttcaaag
     1741 aagagtactg ttccctttga agctccaaaa ctagctaatt tttccttagt tccagcaata
     1801 gctgtctctg gagcaccttg tactttcatt gtttctacca aatcattagc aaccaattca
     1861 tcccagaact tagtaacttc atcattcaat aaaatgacaa cacgggaagt ttgactatta
     1921 aaagcagatg gggattggcg aactgcctct ttaataatct ctactatttt ctcatcagaa
     1981 acttctgtat tgcgaccaag agcgtaaata gagcgacggt tttttaattc ttccaaaaat
     2041 gacatatttt ttctccttgt tcttcaatct cagatatctt actacatttg ttctagtgac
     2101 atatgaatta gattaaagtt atttattact atgtagtaat tatattacca ctatatcata
     2161 aagtcaaaga atgtgactca caaggtttta aattttttta aaaggttaat caagtcttct
     2221 tgctctgttt ctgttaagac tgaaaaagca tcttctacac gtttaatatg ctctggtaga
     2281 gcttttttta tcacttcttc tccttcagtt gttaaactca ccaaaaaggc tctcttatca
     2341 ttcggacaag aatggcgtaa tacccatccc tttttttcca tatttttaat aacaacagtc
     2401 atattacctg atgtagcaag catattttca atcaattctc caattttcat tgtgccttta
     2461 gcatagagaa catccaaaac tgcaaactga gttggtgtta attcaaattc tttaaatgat
     2521 tctgagactt ttccgtctat tgttctaaat gcttttcgca tgacaaccat agatttaacc
     2581 gctgaatttt taaagtttcc catttcatct cccaaacgta ttttttctta ttttatcact
     2641 aattagttat ttcaagcaag aatatcatct ttgccctatc aaaagcagct ggcaaataat
     2701 aaggttgagt agttactcaa ccttattact tagaagaaat catcaaaaag actcaactga
     2761 ttgtcttctg gtaaatttcc caaaataccc atctcatcaa atttttcaac taaggtactt
     2821 gagactcctc ctcgtttacg aagctctgtt tttgataaaa attcaccatc ttcacgtgcc
     2881 cgaactatct gttttgcaac gttttctccc aagccttcca tagcaacaaa tggagggatt
     2941 agcgtatcct cttcaataat aaagtcagtc gcatggctac gataaagatc taatttccca
     3001 aatttaaagc cacgctcaag catttcatta accaactcta atgtcgtaaa aagatcattt
     3061 tcgacattcg tcgcctcatt acgctgacgc ttctcagtga tatccttcat gcgcgcctta
     3121 acggcatcaa gtccagcact cattgtgcgt aattcaaaag ctttggcgcg aatagaaaaa
     3181 taagcacaat aataaaatat tggataatgt actttaaagt acgccacacg taatgccatt
     3241 aaaacatagg ctgctgcatg ggctttaggg aacatatact tgatttttcc acacgattca
     3301 atataccaat ccgggacatt attatcacgc attgcttgga tataaccatt acgctcatct
     3361 tctgaaattt tcaaccataa ccccttacgc acgcgctcca tgattgtaaa ggccatcttt
     3421 ggttgtaggc cagcgtgcat taaataaacc atgatgtcat cacgacaacc aataacagtt
     3481 gataaggtag caataccttc cttgattaag tcttgagcat tacctaacca aacatccgta
     3541 ccatgtgata gccctgacag ttgcaacaat tctgcaaatg tggttggatg ggtttcgttc
     3601 accataccac gaacaaagtt cgtaccaaac tctggaatac caagcattcc tgtaggagtt
     3661 ccaatttgct cttctgtcac tcctaaaact tccgtaccag aaaataactt cataacgtct
     3721 ggatcatctg gaaggatatt tgatggatca attcctgata aatcctgaag tttacgaatc
     3781 atagtaggat catcatgtcc tagaatatct aacttcaaga cattctcatc aatatcatgg
     3841 aaattgaagt gagttgtctg ccaagcagct gtcatatcat ctgctggata ttgaaccgga
     3901 gtaaaatcat aaacatccat atagttagga ataacaacaa taccaccggg gtgttgacct
     3961 gtagaacgtt taactcccgc agcaccagtc gctaaccgtt cgacctctgc atcgttataa
     4021 aatttattgt aatctcgttc gtaacctttg acaaaaccaa aggcagtttt ttcagctacg
     4081 gtacctaccg ttcctgcacg aaaagcatat tcttctccaa agatatcacg aacgtctaag
     4141 tgagcagaag gttgatcatc tccagagaaa ttcagatcaa tatcaggaac cttatctcca
     4201 tcaaatccta agaaagtttc aaacggaata tcctgtccat ctttcttgta caaagtacca
     4261 catttaggac aatttttatt tggtaaatca tagcctgaac cacaagaacc atctgtgata
     4321 aattcagaat gctgacaatt tgggcaaaca taatgaggtg gcattggatt aacttcagta
     4381 attcctatca tggtcgctac aaaactcgac ccaacagacc cacgtgatcc tactagataa
     4441 ccacgttcat tagaacgttg caccaacatt tgcgaagcaa gatagataac ggcaaatcca
     4501 ttaccaagta tagatgctaa ctctttttcg atacgtaaat caataatatc aggaagagga
     4561 ttaccatata tttcgaatgc tttagcataa gttaattcag caactcgttc ttcagcacga
     4621 tcaacaaacg gagtatagag atcccctttt acaacttcaa cgtcttcaaa tcgatctgca
     4681 aaagtattcg tattggttac aacgatctca taggctaaat ctttaccaag aaaggcaaat
     4741 tcatctaaca tctcatttgt tgtgcggaaa tgtgcttttg gcaaaggcgc tggctgagca
     4801 tcctccccac gacctatagt cctattaatc atggcacctt gtccaagact acgaacaata
     4861 atctcacggt aaatctcatc ttcaggctca atataatgaa catttcccgt agctaaaaca
     4921 ggcttatcta aacgccgacc gacctctatt aggtctctaa taatttgttg aatccccacc
     4981 tcatctttaa ttaaaccacg tactaccaag gggcgataaa tagcaggtgg catgacttca
     5041 ataaaatcat aatatttagc tagggtcact gctgcatcaa ttccatttga taacaaggca
     5101 tcaaaaactt ctccatcaga acaggcagta cctagcaaga gaccttctct gtgagcatct
     5161 aaaactgacc taggaattct tgccacccct tcaaaatatt taacatttga aaggctaact
     5221 aacttaaata tatttttaag tccaacctga ttttggacat aaatggttgc atgctttatg
     5281 cgtgcctttt tgtaagagtc ttcagctacc aatttagtat tgagctccat caaattagtc
     5341 acatcacgat tttcccgcgc ctcttttaag aaaataaaga gtaagcgacc cgttgcttca
     5401 gcatcgtagt tggccatatg atgatgttcc aaagcaactt ggaaacgttt ggttagaggc
     5461 cccaaaccat gacgcttata ttcaggataa agatttcttg caaattctaa ggtatcaatg
     5521 accggctgtg tgatcaaagg cagattatga cgttcataat tcgcattcat aaaaccaacg
     5581 tcaaaggttg cattatgcgc aactaaaacc gtaccttgac aaaaattctg aaattcctga
     5641 aggacttgta aaataggttt tgaacctctg acatgattat ccgtaatacc tgtcaattca
     5701 gttgtaaagg cagaaagagg atgacctggg tcaataaact catcaaattg ctcaataata
     5761 ttacccttga acatttttga tgctgcaatc tgaattaggt cattattagc tgcagaaaga
     5821 cctgtagttt ccacatcaaa tacaacataa gttgcttcgt gcaaattcat atcaacttcg
     5881 ttgtaggaaa taggcacttt atcttcaaca atatttgcct ctaatccaaa aattgctttt
     5941 atacctgctt tttttgctct gtggtagcca tgcggaaaac tttgaacatt ggcatggtca
     6001 gtaattgcaa tagctgggtg tccccactta gctgcagtat caatgaggct ctcgacagtt
     6061 ggtaaagcat ccatcgttga catattggta tgagcatgaa attccacacg tttttgatct
     6121 gctggcatta agtctttacg ttcatgatgc acaatctctt tgatatcttg aacattcatt
     6181 gtcaaagatt tagtaaagtt attgttctca atattccctc tgactcgcaa ccacgaacct
     6241 ttagaaatca tatcgtactt cttcaattct tcatcatctt tagcccactt ttgcatagca
     6301 aaacttgagg tataatcagt catcttaaaa ttaatgatat gacgtccagt gcgggttgtt
     6361 tttcgttcaa cactaaaaac cattccttca aaaacgatac gattttcttc tgttgtcacc
     6421 tcaatcatgg gtgtaatctc tgctttttca aagccagctt gacgttgttt gatacgctct
     6481 ttgaaatcat aattttgagt tggggtcact tcttctgatg gaggcgctga atcttctagt
     6541 gatttttgtg cttccaaggc ttgcatagct tcttggttag ctttttcaag caattgctcg
     6601 cgattagttt caaatgatga ttttaagtct tgcgtcattt gttcatcaga aaccatatca
     6661 atagcaagtt taccaaatcc aaacaatgaa aactgttgtt ccaaacgcgg caaatgattc
     6721 tgacgaaaat gattattatt gacaaattga ggtgctgaaa taatcatctg actgccattg
     6781 taatgaactt ttaattgtga aaaagaactc ttaaaacttg cactattaca caagggttca
     6841 caaaaagctt gttgataata atcctgtagc agatcatcag aaaaatcaat agtttctgct
     6901 cttatatcaa aagtagcctt tatatctgct ttttcaaaac tattcactaa acgagtttgt
     6961 aattcacgat acacctcaat tggcaataat tctggaaatg aaaaatgaaa ttcccataag
     7021 cgtgatagag aatgaacctt tacctcgata atatcagcag acgaaaaaac acttgaattc
     7081 ttaatttcaa gcggcatttc aatctgatcc ataagtttct tgaacaattc tgacatagtt
     7141 ttctcctgaa tatcttactt ccattttatc acatatctgt aaatatttcg agataacaaa
     7201 aggaggctgt taaaaaagga atttattagc cttcttaaca acactctctt aactgaaact
     7261 atttatcata agcatctaga ctgtaggttt caataatttg gtttaattta ctagcataag
     7321 cagtatctgt cgcataaaga cctgttagag ctgcagtagc atccttataa gaagaggtat
     7381 ttgatttcca aacagatgca tacttttgac tagatactaa ctcagcataa tcatatagtg
     7441 aagcagaata atttggatag gcgcgaaaag gagcttggat ttgagtcata ttgcctttcc
     7501 catcatcttc taaagtaggc atttggacag atttaccttt atattctcct ttgatgccaa
     7561 agaggttata attaggagcc ttagacaaat ctgattgtcc actggatgat tccaaaatag
     7621 cttgtgctaa taagactgac gcatacaaat cataggtctt agaaatttca atagctgttg
     7681 gagaaatcgt ttgaataaaa gtcgtcgttt gatccgatgc actagtgact gtcggttcga
     7741 cattatcatt tttataatta taaaaccaaa ccccacccaa accaaaaacc aatagtgttg
     7801 ttgttaacct caataccaat ttatcttttt ttcgactttt catgatggct cctttctaaa
     7861 atatcatttc attataacaa aaggcagttg ctttttacca ataagaacca tactaaattt
     7921 cactccatat aaaaaagtta gcacatcaga ctaacttttt tatcggctta tttttttgtt
     7981 agaatctcta aagtttcaat taagttatca gcgtgaacct caatcgtatc accacttgct
     8041 ttaattttca cttcaacgat accttcagaa gctttcttcc caacagttac tcgaattgga
     8101 agtccaatta agtcactgtc tgagaattta gaaccaacac gttcattacg atcgtctgtc
     8161 aggacttcat aacctttaag catcaaatca gcttcaattt tctcagtaag gtcttgtgac
     8221 tcttgatctt tgacattgac agtaatcaaa tgtacatcaa atggggctaa ttcttctggg
     8281 aaattaatcc cccaagcaaa gcgataagct ccttttggtg tcttattcac aaaaagtcgg
     8341 gcatgttgtt caatgacagc tgagagaata cgactaacac caataccata acaccccata
     8401 acaataggat ttgagcggcc attttcatca aggatgttcg ctcccatact atctgaataa
     8461 cgggtaccta gtttaaagat atgtccaatt tcaataccac gagcaaattt cagagtacct
     8521 ttaccgtctg gagaaatctc cccttctttt acttcacgaa tatcaacgta ttcagctgtg
     8581 aaatcacgtt ctggattaac tccagtaaaa tgataaccat cttggttagc accagacaca
     8641 gcattagcaa ggtcttgaac tttacgatca gcaataattt taacagagtc aggtaggtta
     8701 actggcccaa gagaaccaaa acctgcacca aaaatctctt ttgcttgctc ttcacttgca
     8761 ggctctaaga aatcagccgc taaataattt tttaacttca catcattgac ttgatcgtta
     8821 ccaactagta aagcaaccac gggctgttca tcagcaataa acaatagtgt cttgatagtt
     8881 tgattttcat caattgataa aaatccagct acctcatcaa ttgatttgca gttaggagtt
     8941 tctacttttg ttacttcttc aaaggtagca gccttagtag atggtttgta ctcattcgtt
     9001 gccatctcaa ggttagcagc atagcttgat tcagtagagt atgcaatagt gtcctcacca
     9061 gaaactagcc aagccgagag ttctacctta atttcttcta aaacatcctc tggaatatca
     9121 tcaatagaag gaatagtttt atcaaggact aaccaacgat ttaaatctgt acgattggga
     9181 gttactgcca taaattcttg gctatcttta cctcccatag cacctccgtc acctataatg
     9241 cctttaaagt caagtccagc acgtgtgaag atggcttcat atgctttacg gtaatcttcg
     9301 taagtgacat caaggtcttc ataatctttg tggaagctgt atccatcttt catgataaat
     9361 tcacgtgttc gaagaagtcc attacgagga cgcttctcat cacgatattt agactgaatt
     9421 tgataaaggt ttagtggtaa ttgcttataa gattttactg catcacgaac aagagttgtg
     9481 aaagtctctt catgagttgg tcctaagata aaatccgatt gatcacgatt ctttaattta
     9541 taaaggtctt caccatatgt ttcgtagcgc ccagattcac gccataaatc tgctgtaaga
     9601 agagctggag ctaacatttc aactgcacca atcttctcaa attcttgacg cataattgtt
     9661 ttaaattttt caatagtacg gttagctaga ggcaaataag catagattcc agccgaaact
     9721 tggcggacat atcctgcacg aaccataaga gcatggctga taacctgagc atcacttggc
     9781 atttcgcgaa gtgttggaat aagcatttta ctttgtttca ttacaataag atacgactaa
     9841 tcaaagttga ttctttttca attgagaatt gcaattgaaa aattcgtatt tcctttcacg
     9901 ttttattatc ttttagaaga aagctcgcat gatatcgttc caggtcacag caatcattaa
     9961 tactaacata actgctaccc cagcgagcgt aatataagtt tctgtttctt gtttaagtgg
    10021 tttgcgacga atggcttcta aaatattcat cactatctta cccccgtcaa gggctggtat
    10081 agggatgaga ttcataatac ctaagttgat agagattaat cccattaagt tgagaactgt
    10141 gacgaaacca tttttagcag cttggctact agcctgatag agagctactg gacctcccaa
    10201 tttattaata ctaaaatgag caataagttt ctttaattca tttaaaattc taaagaaact
    10261 ttcccatgct agttttaatc cacctaaaag tttgtcttta aagcttgtct ttaaagctgg
    10321 cataatgccg atgatgtagg atttatcaac tttctgtggc ttaacgttga tcgttttcac
    10381 cacctctttg ctcttaatct taagggcaag tttttgtttt ttttctaaat gcctagttga
    10441 cttttctact gctgctgtca actgttccca gttagaaact ttgtgcgacc ctatttgtaa
    10501 aatacgatca ttatttttta aacctgcact agcggcagga ccattttctg atacacggac
    10561 ctgattggtt gataagtctt gaacacctcc ttgaataaaa gcaagtgcaa taaagactac
    10621 aagacctaaa ataaaattat tcatcggacc tgcaaaattg gtaattaagc gcccccatac
    10681 agaagcattc tgatactgca tatctaaggg agcaatacga atttcagtac catcctcctc
    10741 aatgatagta gcatcgtgat caacactata ggtttttgtc tcacttaaga ccaatcctgt
    10801 gatagttagt ttatcttcta aatcataagc tgttacattg attggtaaag aagtattgtc
    10861 taattgtttt cctgataaat taatacgagt tacaatgcct tctttattta aagtaaggct
    10921 agccggcgtc cctgtcttaa tttcagtctt atcatcaccc caaccagcca tacggacata
    10981 accacctagc ggtaatatac gaatagtata ggttgttcct tctttatcaa tgtgagaaaa
    11041 gatttttgga cccataccaa tagcaaattc acgaacaaga attcctgatt tctttgcaaa
    11101 atagaaatgc ccaaattcgt gaacaactac aatgacacca aaaataataa taaaggttaa
    11161 aattcctagc attcaattcc tctctaaata caatatatta ttttagaata agccaaagaa
    11221 gtgcatgatt gggaaaacaa aaatcatact atcaaagcga tccaaaatcc ccccatgacc
    11281 cggaatcaat ttacccgaat ctttaacacc aaaatgacgt ttaatactac tttcaactaa
    11341 atctccaaat tgaccaaaaa tgctaaagat agccactaaa actaacatga ctaggaagct
    11401 atgaggcgca tagacggtct tatcaaaaag cataaaaaag aaagctacca caatggctga
    11461 agcaatccct cctagactcc cttcaattgt tttatttgga gacactgagg gtagtagttt
    11521 gcgttgtcca aattgacgac caatcatata agcaccgata tcagtcgccc agacaataaa
    11581 taaagctaac aacaccttat ctattccagc catacgagca ctgactagat tttgaaagcc
    11641 aatacccaca taaaaactag acgcaattgg aaaggcagca tcttcaaaac tgtaagaatt
    11701 actgttcaaa acagtacctg ccaaaatcat aaaaataaca ataccgtatg ctgataaact
    11761 agcgtcaact ggtaaaaagg acaaataact atctaaagga actgttaaaa caaaggctcc
    11821 tatcatagct aatgcacctt caaaggaaaa tatttctaaa cgacgcatac gaagtaattc
    11881 agaaacacca atcatagcta aaagtccaac taaaaattgg aaaggcaaac cacccattac
    11941 taaaaaaggg ataaatatgg caagcgcgac agctccccaa ataacacgtt ctttcatttt
    12001 ctctcttctt tctatacact accaaatcga cgatggcgtt gattataatc cacaatggcc
    12061 ttatgtagtt cgtctttttt aaaatctggc cataaaacgg gagtaaagta aaattcacta
    12121 taagctgatt gccaaggtaa aaagttactc aagcgtaact ctccactagt tcggataatc
    12181 aaatcagggt ctctataaag atagggtaag ctatttgtca ttaaatgatt tgctactaaa
    12241 tcctctgtaa tatcgtcagg attcaactta gcttccaaaa catcctgagc aatttcctta
    12301 atggcattag tgatttctga acgtccgcca taatttagcg caaaattcaa gacgagcccc
    12361 gaattatgtt ttgtcctcaa acaagcgcgt tgcattgcat cgtatgttgc cttaggtaat
    12421 ttatgagtat ctccgatgac ctgtacacgt acattatttt tatctaactc tggtacatat
    12481 ttatcaaaaa attcaaccgg aaggttcata ataaacttaa cttcatcatc tggacgtgac
    12541 caattttctg tagaaaaggc atagacagtt agtactttaa cacctaatcc tgaagctgct
    12601 attgtaactt cttgaagagc atccatcccc gccttatgtc ccataacgcg tggttttaaa
    12661 cgtttttttg cccaacgtcc gttaccatcc atgataatac caatatgtgc tggtattttc
    12721 tccaaaggca tttctacttg tttttttctt ttaaatataa acataatgtt ccttaattta
    12781 gcaatcgttt ttcttatcag cccttttaaa agagctggaa atatcccata ctataacatt
    12841 ttaccacatt tcaacttatt tttgagtatt aaaatgccat ttgtgacagc aaactaaaaa
    12901 gaccagaaca atcatctggt cctacatatc ttattcagtt tcgatggctg tttctttatt
    12961 ttcgtcttca ttgacttcaa taatcttaac atcttcaact ggttctgttg gtgttgctgc
    13021 cttagagaca acagatttga tagctgccaa ctcaaacgtc aaatagacac catcaacatc
    13081 taaaaccatt cgttgtgctt cagtattaac ttcatcaact actccaaaaa gaccaccaat
    13141 tgtgacaatt tcatcacctt tttgaacagc attcaattgt ttttgacgtt cttgtgcttg
    13201 tttcttttgc tgacgttgca tgaagaacat cataccaacc ataacaacaa gcataataat
    13261 aggtaagctc atttgattct cctttccgtg ttctacgtaa tcattactat agcagattta
    13321 tgctatatct gcaaggagat actgtctctt agccaataaa ttcacggatt tcttcttttg
    13381 tcatttttga atcgcacttc accttgatag ttccgttttt aacaaccatc aaacctggca
    13441 ctgtaggaat gtcatattta tttcgaaatt ctttatttgc agtccaatga tcaaaatctt
    13501 cactatctaa atggaataat tcaaattcat cttcatcagc tacttctaca attttaggaa
    13561 caaagaggtg acaaaatgga caagaaatac gaccaatgaa cagtacaaaa tcttgatttg
    13621 aatcaatttt atcttcaatt tcggcagccg ttatcaaagt catggttgcc aaaagttctt
    13681 caaaagaata catagttaac caactttcta aatcttatat ttaaagaata acctatgatg
    13741 gcaaaacttg caaggcaaga gtaacaacga tcctatcaaa atcattaact cacttctcgc
    13801 ttcaaactat cctcaaaaat gctcactcat atttaaatga ctcatcaaaa aaactgagca
    13861 tagctcagtt tttattttac accttatttt tgtacagcat tactgacgct tttagcaact
    13921 acatcggcca catcattttg aaaagcattt ggaatgatgt gagttgtgct taattcttcc
    13981 tccggtatta aactagctat gcctctcgct gccgcaattt gcatttcaac agttatcgtt
    14041 tttgctctag catctaatgc tcctctgaaa atacccggaa atgctaaaac attattaatt
    14101 tgatttggaa aatcactacg accagttcca actatgtaag ctccggcttt taaggcttga
    14161 tctggataaa tttctggaat aggattcgcc attgcaaata caataggttt atcagccatc
    14221 ttggaaatcc attctgcatg taatgcttcc ggagcagaaa cacctataaa aacatcggca
    14281 ttctccaaag catcttctaa ggatccagat tggaattctc tattagtcaa ttttgcaatc
    14341 gctttgtgat gaggggctaa tgactcccga tctttatcgt taataatgcc aaatcgatca
    14401 actaccgtta catgttttgc tcctgctgat agaagcttcc ttgttataga taagcccgct
    14461 gaacctccac cattaacaac tactctgatg tcttcaatgt cttttttaac tagcttcaaa
    14521 ctgttaaaca aagccgctaa tactacgatt gctgtaccat gttggtcatc atggaaaact
    14581 ggtatatcac attcttcaat taggcgttgt tcaatttcga aacaacgtgg tgctgaaata
    14641 tcctctaaat taatccctcc aaaagtcggc gaaatagctt ttactataga aataatttcc
    14701 tctgtatcat ttgttttcaa cacaattggc acagcatcta catttgcaaa acgtttaaag
    14761 agtgctgctt taccttccat gactggcatt gctgcctcag gaccaatatc tcctaatcca
    14821 agaacagctg aaccatcact cactacggct actgtatttt ttttagtcgt caactcatat
    14881 gcaagctcca catcctctgc aatagctgat gatactgacg ccacccctgg ggtataggca
    14941 atactaagat ccctcttatc ttccacctta acttttgaaa ttacttctaa ttttccacca
    15001 ttttctctag cttggttgat agctaattga cctaaatttt cactcatttt gataactcct
    15061 tttgataaat attagaataa catgcgtaaa atggctgtca ttgtgataac agtaatagct
    15121 cctcctaaac gcgtagcaac ttgagcaaat ggcatcaagt tcattcggtc tgctgtactt
    15181 aaaatggcaa catctcctgt tcctcccata ccactttgac aagctgaaat aatacctgct
    15241 tcaacaggat tcatattaag gaatcgcgat acaaagaatc caacagaaat aactgtaaat
    15301 acaacactaa taacaactat gaaatattgt atgctaagcg ttgccacaac atcttttaac
    15361 gggatataaa gcaatcctag acctgccatt agaggaaatg taaaattacc agaaataaat
    15421 ttataaagtt gcttagctcc attttgtgtt tctctaggaa taacattaat atatttcaaa
    15481 atagctgctg caacaatcat taatacggga ccaggaaatc cagttaattg ctgcaacaaa
    15541 tgtccaaaaa taaagagact acatgctgtt aaaacacctc cacccatttt cttaacgtca
    15601 attgggccag aatgatcagc aataatatct gacatgtcct ctcctttatt taaccttact
    15661 aattgccctt gaccagacaa gtgtggtttc ttttctccca aacgattcaa tagtgcagta
    15721 cataaaatgg caaagaaatt accaataata gtggctggga tgagttgagc aactagttgt
    15781 tcactagcta caccggtaat tgaactatag cctaacgata atggtaaaat accttcacca
    15841 ataccaccag ctaaaacggg tgttacgata taaaacaaag tgtgtcgcca ctctaagcct
    15901 aaaataacac cgacaaatgt ccctaccatc atcgcacaaa ccattcctaa taacatgggg
    15961 aaaatcattc ttagcaatcc ctgaatcaac attttccgat tcatccctaa aatactaccg
    16021 gacactaaac aagcaatata aaaataaaga aagttagctt gcttcatcaa catatttgtt
    16081 gattctaaaa catttttatt aatgaggttg aaaaacacca aaatagatgg tactaataaa
    16141 gataaaatag ctgggccacc gaagttttta aaaccaggaa tgctagctcc gatagtccct
    16201 aagaaccacc ccattgttaa gataactgca aatcctccta gcatattgac tggcaatttt
    16261 tgtaaaaagc ctgctagaag aatcaataat gctaaacaaa catatactgg taggggaact
    16321 gaccctatct taatatgaaa tagtttagcc tttaagtctt gcttttgacc tctagaatcc
    16381 tcattattca ctaccttcac gtctgccatg aaagcacctc cttttacttc ttatgtacag
    16441 tttactttgg gacttagtga taaggaagtt tatttaagta attgaattat ttcaacttat
    16501 ttaattaatt taagctggat tctagttttt aggctatgtt atactaaact cggagggtgc
    16561 tgatgaaaaa aaaactatct ctttgggcat ttctatcatt gattttagtc actatgacca
    16621 tttgtatatt ttctattttt tattatgtca ctatacatca gtcctatcgt atggtaaggg
    16681 tacaggaaga aaaaatctta aaaaatactg gttatgcttt atcgagaaat ccacaagtta
    16741 tacaaacact caaagataat cactatgatc aaagtctcca aaaacaaatg ttattcctta
    16801 gcaaaaaatc aaatttagac tatattgtcc ttataaattt aaaagggatc cgttttaccc
    16861 accctgactc cactaagatt ggtaaaccat ttcaaggggg tgatgaacaa gctgttttta
    16921 agggaaaagc tattatgtct actgcagaag gttctttagg aaaatcttta cgatacttaa
    16981 ttccagtata tgatcaccaa aaacaggttg gggcaattgc agttggctta aaattaacga
    17041 cacttggaga cttatctcaa agtagcataa aagagtttag taagcctctc ttgattagta
    17101 ttttgattag cttagtcgtc acaagtataa tttcttatgg cttaaaaaaa caacttcaca
    17161 acctccatcc aagtgatatt tttcaacatt tagaagagcg caatgcaaca ctggaccaaa
    17221 ttcaagccgc tgtatttgtt attgaccaga ggcatatcat caaaagaaat aatcctgcag
    17281 ctagtttatt attcaaaaaa gaggggcaac gagacctatt ttcaggtaaa ttattagaat
    17341 ctttaattcc tcaactcaag caggatcatt tttcaaaaaa aactgaacaa gtattacatt
    17401 ttcaaggaca agactattta ctatctatct cgccaattac ggttaaaaca caaaatagag
    17461 gctatgttgt cttcttaagg aatgttactg aaacactttt cactttggat caattagcac
    17521 acactacagc ttatgcttct gcgctacagg cccaaacaca tcaatttatg aatcaacttc
    17581 atgttattta tggacttaca gacattgaat actacgacga gttgaaaatt tatttaaaag
    17641 aattattgga gccacaaaat gaatttttag cacgcttatc aatgttagtt agagagcctc
    17701 gactggctag tttcattatt ggcgaaagag aaaaattcgc agaaaaacac atcaatctat
    17761 ccacagaaat tttagtagaa atccctacca agtcaactgt tgaggatgtt aataactatc
    17821 tcttgctcca cagatatatt aatacgaaga tactcacgct attaaattca acgactttag
    17881 tttctttacg tttaaattat caaaataacc tcattgaaac agattatcaa tgggagaatg
    17941 agaagtggtt actcaatgac taccatcagt atttcaacga cgcttatttt caacagttac
    18001 ttgtagattc aagagctact tataagatct ctttaaaaca aaataaggtt attataagaa
    18061 caaatattat ctactcagaa gggccttagt atggacgtat taattatcga agatgatcct
    18121 atggtggaat ttatccaccg caattacctt gaaaaactca attattttca aaacatctat
    18181 tcaactgcta gtcaaacaca agctattgct tatcttaatg acattaaaat ccaacttgtt
    18241 cttttagata ttcatattaa agaaggcaat ggtctagaat tattaaagtt attacgcaat
    18301 caacaccaaa ataccgaagt cattgttatt tcagcagcca acgaggcgca cactgtcaaa
    18361 gaagcatttc atttaggtat tgtagattac ctgataaaac catttacatt tgaacgcttt
    18421 gagtctagta ttgaaaaatt cctaaatcat tatcatacat ttgaagctga taaaatctat
    18481 caagacaaca tcgatcactt tcaaaaaata gattctggat ggctagaggg agaggttaaa
    18541 cttgatgaga aagggctctc tgaaattacc tatcagcata ttcttgatgc cattcaagag
    18601 ttagagcagc ctttcactat ccaagagtta gcaaaatgca gtcaattttc tcatgtctct
    18661 gttaggaaat atattgccta tatggaagaa aaaggccttc tgacaagtca gcaaatttat
    18721 actaaggtcg gtcgtcctta caaagtttat aagcttataa aaaaaaactt aggcctgtag
    18781 acctaagttt ttttaatctt cgtagccatt tggatgacta gaatgccatg cccatgccga
    18841 tgaaataatt ttatcaatat tatcaaattt aggctcccac ccaaggattt gacgagcttt
    18901 ctctgaggaa gcaataagcg tatctggatc tcctggacga cgagctgcct tttgagcagg
    18961 aatttctttc ccagtaatac gacgagctgc ctctaacatc tgaaggttag aaaaacctgt
    19021 cgaagatccg agattaaaca cgtttgattc attcccttgg cgaaggtaat caactgctaa
    19081 tatatgagca tctgccaaat caaatggatg cacataatct cgaacattag ttccatctgg
    19141 agtattgtaa tcatctccaa aaatcattat tttgtcacgt actccttgag caacttgaag
    19201 aatgattggt aacaaatgtg tttctggttt atgatcttcc ccaattgagc catccggttt
    19261 atctccagca acgttgaagt aacgtagagc gacaaactta ataccgtagg cttgatcagc
    19321 ccatttcata attgtttcca tcataagctt actttcgcca taaggattaa taggattttg
    19381 aggagctgtt tctaaaatag gaacttgttc tggaatacca tatgttgcag ctgttgatga
    19441 aaagacaata tttttgatat cacattcatt cattacttct aaaagtttta tcattccagc
    19501 agtgttatta tcaaaatatt ttaaaggatt ttccattgac tcagcaacta gtgaaaatgc
    19561 cgcaaaatgg ataacagcat ctacatcagg attttcccta aaaacttgac gcataaacgt
    19621 tttatcggat aaatctcctt cataaaaaat cgcatctgaa tgcaccgctt gacgatgacc
    19681 tgttaccaag ttatcaacaa caataacttt ctcttttcct tgtgtgatta actgatcaac
    19741 catatgcgag ccaatgtagc cggcgccacc taaaatcaga actgccatat ttttctccta
    19801 tttcaatact cttttaacat catttgttac ctcattatac tgagaataag ctttttttgc
    19861 aaatatctac cgaaattaga atagctctat gcagaaggca tcccacggct tgagagtgtt
    19921 acttgatagt acttcttgaa cctctgtatt tgaaataata gtttctttaa cagcttctgg
    19981 aaattcaaaa gattggtttt gatcggataa gttcgcaact attaaataac gttctttatc
    20041 tgtttgacga acataagcaa ataccttatc tgctgtttcc aaaagtttaa aatcagcatc
    20101 aactaaccag tcgtgttctt tacgtaaagc aactaatttt tgataagtat aaaaaatact
    20161 ttccgtatca tttaacgctg cctccacatt aatttcctga taattgggat tgactgctag
    20221 ccatggggta cctttagtaa aaccagcttg atgttcttga ctccactgca ttggagtacg
    20281 agcattatca cgaccgactt tacgaatact atctaaaatt gttgcttcag ataccccatt
    20341 atccatggct tctttagcat agttaaggga ttcaatatca tctacatctg ctaaacactc
    20401 aaatggataa ttagtcatac caatttcttc accttgatag atataaggcg ttccacgcat
    20461 caaatgtagt aggattgcta gagccttggc agattgtttt ctgttatcac tatcatttcc
    20521 ccagatcgag agcactcgag gaaggtcatg attattccag aataaagaat tccaaccttg
    20581 gccaagctcc aattgtgttt gccattttgt aaaaatatct ttcaatgctg gaacatctaa
    20641 tccgtcactg taatcccact taggagcgtc aggtttatgt tgaagaccta catgttcaaa
    20701 ttgaaagacc atagataatt cttcattatc gggatttgag tattgttttg caatgtctgg
    20761 cgttgctccc caagtttcac ccaccgtcaa taagtcatgc tttccaaaac tagctctgtt
    20821 catttcttta agataatcat gaagttttgg tccattacca gtaatgccct tatctggaat
    20881 tttcccaatt aaatcaatga catccatacg gaaaccacca atacccttat caatccagaa
    20941 attcatcata tcataaattt tatgacgtaa agcttcattc tcccagttga gatctggctg
    21001 acgtttgcta aaaaggtgca aataatattg acctgatact ttatcatatt cccaagctga
    21061 gccactaaaa atagaagtca aatcgttagg ttcatctcgc caaatataaa aatcacgttc
    21121 aggactatta gggttttctc gcgcttctac gaaccaagca tgttcatcag aggtatgatt
    21181 cactaccaaa tccatgatga ttttaatccc gcgttgattt gcctcttgta acagttggtc
    21241 catatcattc atatcaccaa aaatatctgc aatggcttga taatctgaaa tatcataacc
    21301 attgtcatcc attggacttt gataaacagg tgataaccag atagcagtaa tccctaattt
    21361 ttcaaggtag tctagtttgc tagtaatccc tttaatgtcc cccacaccat caccatctga
    21421 atccataaat gaacgtgggt aaatttgata aatcgtagct ttatgccacc agtgtttctt
    21481 cattattctt ctcctatcaa taattcattc atcttttatt ttaacgtttt tcctctttta
    21541 gtgcaagcgt ttgcgtaaaa acattataaa aaggctggaa cccaaccttt ttatgaatat
    21601 cgcttatacc ttaacgaata gcttgttcag tatctgcatc aaagaaatgc cccttagcaa
    21661 cattaaacgt taggttaact ttttctcctg gattatggaa gtcacgcgct tcaacacgag
    21721 aagcaaattc tgtttgacct aacttaacat aaagcatagt ttcgctaccg agtaattcag
    21781 aaactaaaac ttctgcttca acattggcat ttggataagt atcttggaca agaagattgc
    21841 ttgaaatgtc ttctggacga ataccaaaaa tcaattcttt tcctttatag cctcgtgatt
    21901 ctaaaagttt ttcttgacct tcagggatag cgattcgaag accatcttca ctaataatca
    21961 taccattttc aactttaact ttgaagaagt tcatagatgg gctaccaatg aatcccgcaa
    22021 caaatttatt ggcaggaaga ttatagagtt cttgtggtga accaacttgc tcaattttac
    22081 caattgtacc atcaccatca gggttcttag tagctgacat aatgacaata cggtctgcca
    22141 aagtcatcgc ttctgtttgg tcgtgggtaa cataaatagt tgttgaacca atacgttgat
    22201 gaatttttgc aatttcagca cgcattgaaa cacgtaattt agcatctaag ttagataaag
    22261 gctcatccat taagaacact ttggcatcac gcacaatagc acgtcccata gcaacacgtt
    22321 ggcgttgtcc acctgacaaa tccgcaggtt tgcgctctaa gaattcagta aggccaatat
    22381 tagctgctgc ttcacgaacg cgcttatcaa tttcttgttt tgagaattta cgcaatttga
    22441 gcccaaaagc catattatcg taaacagtca tatgtggata aagagcatag ttttggaaaa
    22501 ccatagcaat atcacgatct ttaggtgatt tgtcattgac aacttcgcca tcaatcttga
    22561 gctcaccttc actaatatct tcaagacctg cgatcatacg aagtgttgtt gatttcccac
    22621 aacctgatgg tccaacaaaa acaataaatt ctttgtcttt aatatccaag tcaaagtctt
    22681 caacagaata atgcgatgca cttggatatt ttttgtaaat atgatttaaa tttaattcta
    22741 ccattagatt gacctctttt attattgtga ttttattata gcaaagaaag ggttttcata
    22801 taatggtaac cttgcctaaa tttcttgtgc aaactaacta aattttaggt atccatcgaa
    22861 atcaaatagg cataggctaa atcatctaat atttttaaat taagtcccga agattgggta
    22921 aatttatcta atttatattg caatgaattc cgatgaatga aaagtgcctt agccgtttta
    22981 gcaagatttc cttgctcttg ccacagggct tttattatag ccgaagtatc actcgtatca
    23041 aggatatgtt gtaaacattt tgctttaata gaaggcatgg gcgattgatt tgccaaagcc
    23101 cataaagcca tctttggaaa tcgacagtaa tgttgttcgg ccttgtgact aagaaaaagc
    23161 gtaaaaagtt tatattcttc ttcatataat tctttccagt ccactgcttg tagatgcgtc
    23221 caactatttc caaaatagca ccttaaacgc gtgttaaagt cattttcaat cgttggtaaa
    23281 atatctgtta gtaattccgt agcattaaag aaattatcct gatttaaaat catgacactt
    23341 tgggtttgat taattgtcat aattgtttct acgtttatta ctaaaggcga caagaaatcc
    23401 ttaagttctt ctgataaggt aaattgatgc tcaataaaaa taaattggac tgccgaatga
    23461 gattggggca accttccacg accttcaact agatagcggt accaaggatt ctgagaatcc
    23521 actgaactcg catcttccag gcttggtgtc atttgtaata agtatagttc acgcttagtt
    23581 agcgaagatt ttggaaagtg gaggacatga ccatcttgct caacgatgat ataatcctca
    23641 ctctgtttaa cttcctgttt agtaataact tctggaaaca gttccttaaa atccatggca
    23701 tcctccacta cagttttgtt cattatatca tatttataac ctctttttga gcacagaaaa
    23761 agaggagaat gtctcctcag aaaatttctg ttataaaata gtgatccgaa tagaatgctg
    23821 tttcgactgc tttggattaa gccgtacgat attttgttta tcttctaaaa tatcagactc
    23881 ttcaatagaa gttgacaaag aactccaggg ttccaaagcc aaaaaaggac caccattgtt
    23941 acttgtccaa agaataagat tctcaaaatc atcaaagtca agttggattc cttttgcatg
    24001 atttctagat tttaagtaaa ccgtcttaga tcgtagttgg tctaaagtaa tagcatcttt
    24061 ttcaaataaa gcatgattaa gagataattg tttttgattt tctaaaaaag gatgtctagc
    24121 ttgtaaatct aacaagcctg tatccggaaa cgactcagga attgtgcaag tttcaaactg
    24181 ttcaaactct agatagtaat cactaaatac ttccttttca aacaaagggc aattaaagcc
    24241 tgggtgagct ccgatagcat atggcatatt tttttctgat tctaagtttt tgacaatata
    24301 tgtaatggca atgcttttat ttcttaaggt gtaagttatt tctaaactaa aacgataagg
    24361 ataatttatc aacatttctt tactactttt aattgtaaaa cgaacagaag aatccgatat
    24421 gtaatcatag tcgaatgttt tgtgacgtac aaagccatgc cttggtatga tacccgtaaa
    24481 atgtggagct tgagctggtc tgtaaatgac gcaatcattt cgaacacttc cgcatatagg
    24541 aaataaaata ggagcttgcc caccccaata agtggcgtcc ccttgccaaa ggtactcaac
    24601 gccttgacta tctgtaatag aagtcattgt tgcacctaac gcttttaatg tcacttgcaa
    24661 ctcatgattt tgtatcgtaa tagccattat ttttcctttc ataaagaaca ataattgctt
    24721 taccagcttt tctctagcaa ttatctttcc ttccaagaag ttgctgtttt ttgcaaaatt
    24781 ttatttagtt catcaatatt ttgaaaacct attgtacgta accattgacg tgcaccagct
    24841 tctccttttt caacatactc tttaacactt ccagcccaag ttgctctacc acaaaggaca
    24901 ccgttaaatt tagcaccagc ttctttagca aacacaagag tctcttgaaa aagctgagca
    24961 ggaactcctg cactcaaaaa aatgtagggg agtagtgtcg cctgatcttg ttctctaaaa
    25021 tatgccgcag ctgtcgcttt attataagca gtctctcctt gcgcaaaacc ttctacatag
    25081 tccatattga caggcacttc caccttcaag acatcaatat taaatcgagg atttgaaaaa
    25141 actttcatag cttcaataac cttacgtggc ttaattttgg catactcaat acctgagcta
    25201 tccgtaatct tttcatcata tgacaaaatt tcaaggaaaa aaggaatgtc ttctgcaaca
    25261 cattcagaac caatccgttc aatataagct tctttttctt cgtttacttc atccgaacta
    25321 tcaacatcat aataaagtaa gaacttaact gcgtctgctc cctcttcttt aatgcgtttc
    25381 gcagaccaaa tgtctaaaca gtctgggagt cgcttggtac tactggtatc ataacccgtt
    25441 ttttcgtacg caagtaaaag accggcattg tcatctaaaa ctttcgtagc tggcaaccca
    25501 tattctggat ctaacaacat tgatgaagcg taaggggtta attcttcagc taccaaaact
    25561 ttaagtgctt ctatttgaga aacagtgggc tcctcagatt gatattttgc cattaatcgc
    25621 ttcaatgctc cacgttggtc aaaagccaag gccgaaataa taccattttt atcactcaat
    25681 tgttccatat gtttttgttt ttgttctgtt aatcccatcc tatacctctt tcacttggat
    25741 ttgactgatt aattcttgat agtaggtcag attgatatgc cctgttaatg tttcttgtgc
    25801 attaagcata cccaagacat tagctctctt aagcaaattt gtatcatcta gttgatgttc
    25861 caaagcagac gcgattcctg caaccgttgc atcccctgaa ccaactggat taataacttc
    25921 tatttttgga atcgtcactt gatagtagtg attcccatgt ttagcaaaag ctcccctagc
    25981 acctaaagaa acaataaccc aatcaattcc tctaaaaaga tcttgctgta aggtagattt
    26041 taattcgtct aaactatagc taattggttt tccaattaat tgtgttaatt cttcaaggtt
    26101 aggtttaatg acggtcggct tagcactaga ttttaaaacc gaacgtaatg cttctcctga
    26161 acagtctaaa acaactttct tattaaagtg attcgctagg gagattaacc tagcataata
    26221 atctgagggg agtccctttg gtaaacttcc tgatactgtt atgatagttg cagcatcaaa
    26281 gataagtttt agatgattca aaaaggcgtc tgcttcatct ttagtaatat gtggaccttt
    26341 ttctagaatt tcagtctgct gaccctcatg taaaactgca atacaatgac gtgtttctcc
    26401 accaatactt aaaaattggt gtctgatttc caatgcatct aagccacttc ggatgaaatc
    26461 tccaaaatcc ccccctaata gaccagttgc cactacatta tcgccaagtt gagaaagaac
    26521 acgagtcaca tttaacccct tacccccagg tgttttggag acgtcggtaa ctcgatttac
    26581 agtatccatg ttaaagtttt ctaagcagta cgaaatatca atagaagggt tcaaagttac
    26641 tgttaaaatc atagatatct ccttagtcgt gatattcacc acgattccat ttatctaaga
    26701 actctgtgaa aaagtcattc tcttcttgat gggcattatg agtctcaacc tcggctatct
    26761 tttcaattaa tactttgttt tcttttgttg gtttatattt agctcttata aacgcctcga
    26821 tgatatctgt cattaacagc ccaccagtaa ttttcccacc aaatccgata acgttagcat
    26881 ttaattcttc tttggcatag atagctgatg tcaaatcacg taccagagct gagcgaattc
    26941 ctggaacttt attaacagca ttattaatac caactccagt tccacaaata catacaccta
    27001 agtcggcttt tccactagcg acagcttctc ctactttctt accataaata ggatagtgag
    27061 tacggatatt atcataagtt ccacaatcaa taacctcata cccttttgtt tttaaataat
    27121 ccacaactgc aattttgtca taagttacaa tatgatcaca accgaccgca attttcatct
    27181 gctttacctc tcttacgctt agcacatttt attcaacata tcgacccgaa tttgatgacg
    27241 acctgcatcg taagtcccat caacaaaacc ctctactata tgtttagcaa ctcctggacc
    27301 aacaatctct gaacctaaag taatcatcct agcgttatta tgagctctag tcatataagc
    27361 tgagcgttca tctgaaactt ctgctgcaat catcccctta accttagtgg ctaccataaa
    27421 aggaccaaca ccgtaagcat caaccattat tcctaagtta tcttcttttt gattcacttt
    27481 agcaacgatt gctaaggtat tatcaacgaa atctttgcta gtatccgtta aatcaataat
    27541 ttccttacct aaacttgtaa ggtgttgtct aatcacttcc ttaagttcaa caccatgcgc
    27601 atccgcccca ataataattg tcatttcttt ctccttattt cacttttaat acaattgtat
    27661 gtagttggaa gatattgttt gtttttgttt gtaatatcat catatatctt atttttgttt
    27721 ttgtcaatgt tttgttttgt ttttgtttga aatgaacaaa aaaaactccg tgataactat
    27781 gtcaactaaa attcactact agctagctat cacgaagttt gatacttctt attattttta
    27841 tttgtaggaa cgcttattcc gcaagccata atcataaata aaatttaaac aaaacatgcc
    27901 aatttgctgc cattttctcc cataaagtgc cttgcttctc tgtattttga ctaagaaaac
    27961 tccaattaaa aactttgtaa tttaaagtaa aactattttg atgtttttct ctatgttcat
    28021 aaaggacacc aaatttatca ttattaagtt gttgaacaga gttgtaagca aacgaaccat
    28081 cttgaatgtg atggtgatat aaccacttaa aactaccatt tcgattaact tgcgctagac
    28141 gtgcatatcc atcttgacgc ttcttacctg gcccattagc attcactaat aaaatatact
    28201 cttttttgtc atgctcaaag cgaatagctg ataattggac gtaagcatca tgaacttcct
    28261 tatatcgttt aacatggttt tgccaagtct ccccgccgtc tttacttgtg gctacttcta
    28321 ggttaccagt tagattcctc ataaataact taatgtcccc atttttcaat tgaacgggta
    28381 cggattctgt attttgttct tttttattat ccattgttaa ggagtgaatt ttttcaccat
    28441 tagaaagtat acggttatca ttgactgctt ttccagtatg ccacgttttt ccatggtcgt
    28501 ctgaataaat taggcgtgaa gattgtgaac ctcttagatg agatttccaa ttcgtagaat
    28561 aggcaggaat aataatacga ccagcgtgtg gcccccattt taaaactata ccttttccag
    28621 gtcctattcc taaaaatttc atgcctttct gacgaagact tgctgttata tctctaggtg
    28681 atgaccatgt cctaccatca tcatcgctat atgacatcca cacatagctt gattttgcta
    28741 aacgaaatgg tgacgtttta tgttttgtaa aatatatatt tccaagtagc tgttttccct
    28801 tataaatatc ccctagattt gaatggtcac ttttagtagt ttctgttata acatgataat
    28861 cagtcttttt ccctttaaaa ttatatacaa tacctttgtc acggatagta aaaaccttcg
    28921 atttctttcc attattatat aaattaagat aagactgtcc tccgatttgg gtatattctt
    28981 tttcaggtgt gttagcaata ctaataacgc ctcttccttc tggaaacata tcatatattg
    29041 aaaaaatacg tttcgtctta gagctagtat cttgaactaa tgccatatcc atattaatag
    29101 gtgagccact atagtcacca ctagtaacta gcggaactct agggttatta cggagattga
    29161 caatagtttc tctttttccc caagtgacac catcatcctc actacggcga ataaccattc
    29221 ctatgtttcc ccaatcacaa gcttgctcaa ttctttcatc agctcccaca agtaaagttc
    29281 ctttttccgt ttttaataat gaggggatac ggtaaccaaa cacattatct ttattttttc
    29341 tcccaaaatc tcctccccta tatacttctg ttttagcaga aagtatagca cctttaggta
    29401 atttgagctg atggtttaga tgacaataat attgaaaata agattttata tcttcaaaat
    29461 tcaaattatc aacacagcaa acaaggcagt gtgccttagc ttttaatttt tgccataaat
    29521 taccactata ttttatttcc ttctctactt tctctttgcc aatagcaagc atcccctcta
    29581 taacagacga taactttcca tccttttgag agaatgtttt agtaagatgg tgttcctgta
    29641 tcgcattctt tttaaagccc aacgatatct gactagcaat gtccgtatta cctatgacat
    29701 tacttgtcga taaatctccc ttaggaatga tatccaaaga cctcaactta tccctttcaa
    29761 aacctctatc ctctctcgct gtctttctaa attcatcgct attaagtaat gcaaccgaat
    29821 aaccctttga ttctatcagg aatctctgtt gttctgcaat ggaatgactg ctttcgggta
    29881 taatataggt agttctatta ttaggttgag ttgcatttag ttctgctaaa tttgcctgtt
    29941 tacgattttc ttctgtcgct atttccgatt ttagaggagg tgacaatttt tcttgactgt
    30001 cagaatcacc taagactatt accatatctt tatctttttg atcatctact attaaactgt
    30061 ccgatgtatc agaatctaat tctaactgcc tctcctctat tgaattagta agcttatcta
    30121 aagtagagtc aaatatggga acagtagttt tttcatcatc tattgatata aaagagttta
    30181 tatcagattt tatttcctca ctataaattg tactaggatg caaaaatcca cataataaga
    30241 taaaacctag catcatatat gtactagtta tcactcctag acggcgtatc gttatagaag
    30301 gtttcccaaa ggttaatgtc atagaaactc caagtcttaa aatattggtt gcatacatca
    30361 actctagaaa aatgggagtt cagtaatgtt tccaaaaagg attgtatacc aacttatcac
    30421 tattttcaat atatttttat aattttacta gttttttat
//
LOCUS       DATRMA010000017        28967 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.017, whole genome shotgun sequence.
ACCESSION   DATRMA010000017 DATRMA010000000
VERSION     DATRMA010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 28967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28967
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.017"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            117..2681
                     /locus_tag="VBC28_000730"
     CDS             117..2681
                     /locus_tag="VBC28_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GBS Bsp-like repeat-containing protein"
                     /protein_id="HEO6814731.1"
                     /translation="MRPIVRKKMYKKGKFLVIAGIVTILGGSAILGQDVKAEQAEAAT
                     STISEKTDSSQTISDTSKLTLPVNSSEAMKNSAEPLIKTGFATSVSSNPREIAATPVK
                     TFDASSKVVVKASTAEHSANQTNSNVNQVANDSEVITQQNSTKQLPKVTYSAHVQDIG
                     WQKSVDNATVSGTVGQEKQVEAIKLSIKAPEGITGKLSYKTYVKGQGWQPSVESGQVS
                     GTVGQSRPIEALSINLTDNLQKLYDVYYRVHVQDIGWMAWAKNGAYAGTLGMSKRLEA
                     YEVKFTLKGQSVLTPTILKEERPVLNYQVKVGQNGWQSNKLEGQMAGTLGESKALDGV
                     KFTLSTLKYGDILYRTHVQDKGWGEILGSQTSKDYQGKRLEAIQLDLSGNLKQNYDIY
                     YRAHVQDKGWMAWQKNNSVAGTVGESKRLEALEVKLVAKASDFSSQTNHSFLEMKHPG
                     LSYQTYLQKDGWKPTVLEGQLGGSIGLSKSIKAIKLNLGSTALGNIEYRTFLNGSGWQ
                     TVVNSGRESNVPNESQQVEAIQMRLTGLLSKTYSVMYRVHMQDYGWQDWTYNNDIAGS
                     TNQGKRIEAIEIKLVETAKIPITVQTTYKGTGNYNVRVTNVISPGNLKIAIWSDKNNQ
                     DDLKWYTVTPKNHEARLTFNVTNHRDNGKYFIHVYQENASNQKQLLVKTSLQVAHSNY
                     NTPYFSQRDGRWASRRYGMATLGETGCVPTSLAMILSSLKGETVLPTQIADYLYHKTV
                     EFNRGVQGTTSRGILKAAKEWGVTATALGTQANLVQALKDGYHVLAAVQNNVFVLHGS
                     HEIVLKGYNNGLTHVSDPYTPSLSGWYPISQLWKEQSYYSEDRIDIGEPFVKVTDA"
     gene            2804..3322
                     /locus_tag="VBC28_000731"
     CDS             2804..3322
                     /locus_tag="VBC28_000731"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEO6814732.1"
                     /translation="MDLNNYIASIENYPQEGITFRDISPLMADGKAYSYAVREIVQYA
                     ADKDIDMIVGPEARGFIVGCPVAYALGIGFAPVRKPGKLPREVISADYEKEYGLDTLT
                     MHADAIKPGQRVLIVDDLLATGGTVKATIEMIEKLGGVVAGCAFLVELDGLNGRKAIE
                     GYDTKVLMNFPG"
     gene            3440..4120
                     /locus_tag="VBC28_000732"
     CDS             3440..4120
                     /locus_tag="VBC28_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HEO6814733.1"
                     /translation="MTYLEQYQSGQLTLPSALFFHFKSIFKTADDFLVWQFFYLQNTT
                     NLSDLTPSRIATSLDKTVADINRSISNLTSQGLLDVKTIELNHEIEIIFDTSPVFAKL
                     DKLFEEDNQVIIDNKTADSNRLKDLVGDFERELGRLLSPFELEDLQKTLQEDQTDPDI
                     VRAALREAVFNGKTSWNYINAILRNWRREGLTTLRQIEERKQAREDNQMKDLAISDDF
                     KNAMNLWE"
     gene            4224..4907
                     /locus_tag="VBC28_000733"
     CDS             4224..4907
                     /locus_tag="VBC28_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000365681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HEO6814734.1"
                     /translation="MDLQLSKRLQKVANYVPKGARLLDVGSDHAYLPIFLLQMGYCDF
                     AIAGEVVNGPYQSALKNVSEHGLTSKIDVRLANGLSAFEEADNIDTIAICGMGGRLIA
                     DILNNDIDKLQHVKTLVLQPNNREDDLRKWLAANDFEIVAEDILTENDKCYEILVVKH
                     GHMNLTAKELRFGPFLLSNNTTVFKEKWQNELNKLTFALNSIPNSKMEERAILEDKIQ
                     DIKEVLDES"
     gene            4897..5685
                     /locus_tag="VBC28_000734"
     CDS             4897..5685
                     /locus_tag="VBC28_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEO6814735.1"
                     /translation="MKARELIDVYETYCPQELSMEGDISGLQIGSLDKEIKTVMVALD
                     VRETTVAEAIERQVDLLIVKHAPIFRPLKDLVATPQNKIYIDLLKSDIAVYVSHTNID
                     IVPNGLNDWFCELLDIQYPDILSETSNGYGIGRIGDVRPQSFEFFAWKIKDVFGLDSV
                     RLVSYDKSNPEIQRVAICGGSGQSFYKEAIAKGADVFVTGDIYYHTAQEMITNGLLAI
                     DPGHHIEVLFVSKIATMIEQWKLEKGWDISVLESKAPTNPFYHM"
     gene            5694..6797
                     /locus_tag="VBC28_000735"
     CDS             5694..6797
                     /locus_tag="VBC28_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEO6814736.1"
                     /translation="MKKIAIIGAGAVGATLAYYLSKEKDIQVTVFDYGVGQATKAAAG
                     IISPWFSKRRNKAWYRMARLGADFYSKLVTDLQKDGFETKFYQQTGVFLLKKDESQLE
                     SLFALADKRRLESPLIGDLQILNKSEANTHFPELDGYEQLLYASGGSRVEGADLTRIL
                     LEASGVNVIKDEVHFTITDNGFRVQGIDFDKLVLASGAWLAKILDEHNYQVDIRPQKG
                     QLRDYYFSNINTGKYPVVMPEGELDIIPFDNGKVSVGASHENDMAFDLNIDFKVLDKF
                     EEQAIGYFPQLKKADTTSERVGIRAYTSDFSPFFGPVPCMEGAYAASGLGSTGLTVGP
                     LIGYELCQLILNKENQLNLEDYDITKYVKKISR"
     gene            6856..7725
                     /gene="rfbA"
                     /locus_tag="VBC28_000736"
     CDS             6856..7725
                     /gene="rfbA"
                     /locus_tag="VBC28_000736"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002276675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEO6814737.1"
                     /translation="MKGIILAGGSGTRLYPLTRAASKQLMPIYDKPMIYYPLSVLMLA
                     GIKEILIISTPQDLPRFEDMLGDGSELGISLSYAEQPSPDGLAQAFIIGEEFIGDDHV
                     ALVLGDNIYHGPGLSAMLQRAASKESGATVFGYQVKDPERFGVVEFDTDMNAISIEEK
                     PAQPKSNYAVTGLYFYDNDVVEIAKNIKPSPRGELEITDVNKAYLDRGDLSVELMGRG
                     FAWLDTGTHESLLEAAQYIETVQRMQNVQVANLEEIAYRMGYITREQVLELAQPLKKN
                     EYGQYLLRLIGEA"
     gene            7725..8318
                     /locus_tag="VBC28_000737"
     CDS             7725..8318
                     /locus_tag="VBC28_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018379055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HEO6814738.1"
                     /translation="MTEQFFDKELTCRPIEAIPGLLEFDITVRGDNRGWFKENFQKEK
                     MIPLGFPESFFEADKLQNNISFNKKNTLRGLHAEPWDKYVSIADEGRVIGTWVDLREG
                     DSFGNVYQTIIDASKGIFVPRGVANGFQVLSDKAAYTYLVNDYWALELKPKYAFVNYA
                     DPNLGIQWENLEEAEVSEADKNHPLLKDVKPLNKEDL"
     gene            8525..9571
                     /gene="rfbB"
                     /locus_tag="VBC28_000738"
     CDS             8525..9571
                     /gene="rfbB"
                     /locus_tag="VBC28_000738"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEO6814739.1"
                     /translation="MTEFKNIIVTGGAGFIGSNFVHYVYNNHPYVHVTVLDKLTYAGN
                     RANIEAILGDRVELVVGDIADAELVDKLAAKADAIVHYAAESHNDNSLEDPSPFIHTN
                     FIGTYTLLEAARKYDIRFHHVSTDEVYGDLPLREDLPGNGEGPGEKFTAETKYNPSSP
                     YSSTKAASDLIVKAWVRSFGVKATISNCSNNYGPYQHIEKFIPRQITNILAGIKPKLY
                     GEGKNVRDWIHTNDHSTGVWAILTKGRIGETYLIGADGEKNNKEVLELILEKMGQPKD
                     AYDHVTDRAGHDLRYAIDSTKLREELGWEPQFTNFSEGLEETIKWYTENETWWKAEKD
                     AVEAKYAKTQEVIK"
     gene            complement(9623..12841)
                     /gene="hylB"
                     /locus_tag="VBC28_000739"
     CDS             complement(9623..12841)
                     /gene="hylB"
                     /locus_tag="VBC28_000739"
                     /EC_number="4.2.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_192884539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyaluronate lyase"
                     /protein_id="HEO6814740.1"
                     /translation="MEIKKKHRIMLYSALILGTILVNNSYQAKAEELTKTTSTSQIRD
                     TQTNNIEVLQTESTTVKETSTTTTQQDLSNPTASTATATATHSTMKQVVDNQTQNKEL
                     VKNGDFNQTNPVSGSWSHTSAREWSAWIDKENTADKSPIIQRTEQGQVSLSSDKGFRG
                     AVTQKVNIDPTKKYEVKFDIETSNKAGQAFLRIMEKKDNNTRLWLSEMTSGTTNKHTL
                     TKIYNPKLNVSEVTLELYYEKGTGSATFDNISMKAKGPKDSEHPQPVTTQIEESVNTA
                     LNKNYVFNKADYQYTLTNPSLGKIVGGILYPNATGSTTVKISDKSGKIIKEVPLSVTA
                     STEDKFTKLLDKWNDVTIGNHVYDTNDSNMQKINQKLDETNAKNIKTIKLDSNHTFLW
                     KDLDNLNNSAQLTATYRRLEDLAKQITNPHSTIYKNEKAIRTVKESLAWLHQNFYNVN
                     KDIEGSANWWDFEIGVPRSITATLALMNNYFTDAEIKTYTDPIEHFVPDAGYFRKTLD
                     NPFKALGGNLVDMGRVKIIEGLLRKDNTIIEKTSHSLKNLFTTATKAEGFYADGSYID
                     HTNVAYTGAYGNVLIDGLTQLLPIIQETDYKISNQELDMVYKWINQSFLPLIVKGELM
                     DMSRGRSISREAASSHAAAVEVLRGFLRLANMSNEERNLDLKSTIKTIITSNKFYNVF
                     NNLKSYSDIANMNKMLNDSTVATKPLKSNLSTFNSMDRLAYYNAEKDFGFALSLHSKR
                     TLNYEGMNDENTRGWYTGDGMFYLYNSDQSHYSNHFWPTVNPYKMAGTTEKDAKREDT
                     TKEFMSKHSKDAKEKTGQVTGTSDFVGSVKLNDHFALAAMDFTNWDRTLTAQKGWVIL
                     NDKIVFLGSNIKNTNGIGNVSTTIDQRKDDSKTPYTTYVNGKTIDLKQASSQQFTDTK
                     SVFLESKEPGRNIGYIFFKNSTIDIERKEQTGTWNSINRTSKNTSIVSNPFITISQKH
                     DNKGDSYGYMMVPNIDRTSFDKLANSKEVELLENSSKQQVIYDKNSQTWAVIKHDNQE
                     SLINNQFKMNKAGLYLVQKVGNDYQNVYYQPQTMTKTDQLAI"
     gene            13058..13540
                     /locus_tag="VBC28_000740"
     CDS             13058..13540
                     /locus_tag="VBC28_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEO6814741.1"
                     /translation="MTKLATICYIDNGKELLLLHRNKKENDVHEGKWISVGGKLEAGE
                     TPDECAKREILEETHLTVKKMDFKGVITFPEFTPGHDWYTYVFKVTDYEGELISDDES
                     REGTLEWVPYDQVLSKPTWQGDYEIFKWILEDVPFFSAKFVYDEHQNLIEKTVNFYEK
                     "
     gene            13530..13991
                     /locus_tag="VBC28_000741"
     CDS             13530..13991
                     /locus_tag="VBC28_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEO6814742.1"
                     /translation="MKNRDFRVRDQLETFGVRVSALIIENQKLLLIYAPHLDKYYLPG
                     GALQVGEDSNKAVAREVLEEIGLHSQVGDLAYIVENQFNIKRHHYHSVEFLYFVNLLG
                     QAPESIKEGTHKRHFVWLPIKELTKIDCNPNFLAQDLIEWPGHVVHKIVQN"
     gene            14083..15273
                     /locus_tag="VBC28_000742"
     CDS             14083..15273
                     /locus_tag="VBC28_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEO6814743.1"
                     /translation="MNRPSEKEFKNSLFFKWILNNQAVIALMITFLVFLTIFIFTKIS
                     FMFKPVFDFLAVLILPLVISGLLYYLLKPMVTFLEKRGIKRVTAILSVFTIIILLLIW
                     AMSSFIPMMSNQLRHFMEDLPSYVNKVQMETSSFIDHNPWLKSYKGEISSMLSNISSQ
                     AVSYAEKFSKNVLEWAGNLASTVARVTVATIMAPFILFYLLRDSRNMKNGFLMVLPTK
                     LRQPTDRILREMNSQMSGYVQGQIIVAITVGIIFSIMYSIIGLRYGVTLGIIAGVLNM
                     VPYLGSFVAQIPVFILALVAGPVMVVKVAIVFVIEQTLEGRFVSPLVLGNKLSIHPIT
                     IMFILLTSGAMFGVWGVFLSIPIYASIKVVVKELFDWYKAVSGLYTVDVVTEERSEEV
                     KNVE"
     gene            15263..16489
                     /locus_tag="VBC28_000743"
     CDS             15263..16489
                     /locus_tag="VBC28_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6814744.1"
                     /translation="MLNSEKMIVSIQNQDLEHANKYFEKALKNDPEEVLLELGAYLES
                     IGFLPQAKRLYDQIRPNYPEVAINLAQIVAEDGDIEQAFLYLDYISEDSQEYVSALLV
                     MADLYDMEGLTDVAREKLLLASKLSDDPLVTFGLAEMNLSLEHYQEAIEGYASLDNRE
                     ILETTGVSTYQRIGKSYAIMGKFDAAIEFLEKAVDIEYDDLTVFELATILYDQEEYQK
                     ANLYFKQLDTINPDFDGYEYIYGLSLREEHKSEEALRLVQQGLRKNSFDGQLLLLASQ
                     LSYELHDVSSSESYLKQAEKVSENQDEIVMRLSNLYLEEERFEEVLELDNDDLENILA
                     KWNIAKAHKALEMDDSVDYYQSLYNDLKDNPEFLQDYAYILREFGYLDKAQEVGKAYL
                     KLVPDDIEMSEWVNNI"
     gene            16599..18281
                     /gene="alsS"
                     /locus_tag="VBC28_000744"
     CDS             16599..18281
                     /gene="alsS"
                     /locus_tag="VBC28_000744"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019804322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HEO6814745.1"
                     /translation="MTESHNQYGADLIVDSLINHDVKYVFGIPGAKIDRVFDTLEDKG
                     PELIVARHEQNATFMAQAVGRITGEPGVVIATSGPGISNLATGLVTATDEGDAVLAIG
                     GQVKRGDLLKRAHQSMNNVAMLEPITKYSAEVHDPNTLSETVANAYRLAKSGKPGASF
                     ISIPQDVTDSPVSVKAIKPLSAPKLGSASVLDINYLAQAINNAVLPVLLLGNGASSEG
                     VTAAVRRLLDAVKLPVVETFQGAGIVSRELEDETFFGRVGLFRNQPGDMLLKRADLVI
                     AIGYDPIEYEARNWNAEISARIIVIDVEQAEIDTYFQPERELIGDMAHTLYLLLPAIK
                     GYELPEGSKEYLKGLRNNIENVSDVKFDRDSAHGLVHPLDLIDVLQENTTDDMTVTVD
                     VGSHYIWMARYFKSYEARHLLFSNGMQTLGVALPWAISAALLRPNTKVISVSGDGGFL
                     FSAQELETAVRLHLPIVHIIWNDGKYNMVEFQEEMKYGRSSGVDFGPVDFVKYAESFG
                     AKGYRVDSKDSFEETLKQALIDAENGPVLIDVPIDYKDNVTLGETILPDEFY"
     gene            18295..19014
                     /gene="budA"
                     /locus_tag="VBC28_000745"
     CDS             18295..19014
                     /gene="budA"
                     /locus_tag="VBC28_000745"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HEO6814746.1"
                     /translation="MSETVKLFQYSTLSSLMAGLYKGSLTIGELLTHGDLGIGTVHMI
                     DGELIVLDGKAYQAIGTDGKAEIIQLSDDVTVPYAAVLPHYIQKQFDINAETDNKDLE
                     EMILKNFEGQNLFKSLKIKGTFSRMHVRMIPKSLQHKRFADIASNQPEFTRENVSGTL
                     VGIWTPELFHGVGVKGFHVHFISDDLTFGGHVMDYSLTQGKVEIGKVDQLDQCFPTQD
                     QEFLEANFDLQKLREDIDLSE"
     gene            complement(19068..20723)
                     /locus_tag="VBC28_000746"
     CDS             complement(19068..20723)
                     /locus_tag="VBC28_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HEO6814747.1"
                     /translation="MSFDGFFLHHLTNELQEQIEKGRIQKVNQPFDHELVLTIRNNRR
                     NYKLLLSAHPVFGRIQTTEANFQNPQNPNTFTMIMRKYLQGAVIETIQQIENDRILEI
                     IVSNKNEIGDHIKATLVVEIMGKHSNIILIDKNEHKIIESIKHVGFSQNSYRTILPGS
                     TYIAPPKTKAINPFDISDQTLFELLQTNDLSPKNLQQLFQGLGRDTALELSHCLKDNK
                     LNDFRQFFSREYYPSLTEKSFSAVQFSSSHETFQSLGQLLDYYYQEKAEKDRIAQQAS
                     DLIHRVQSELEKNIKKLAKQQDELLATENAEEFRQKGELLTTYLSMVPNNQDVVVLDN
                     YYTNQTIEISLDRALTPNQNAQRYFKKYQKLKEAVKHLKGIISDTENTITYLESVETS
                     LNHASMEDINDIREELVETGFIKRRAHDKQHKRKKPEQYLASDGKTIIMVGRNNLQND
                     ELTFKMARKGELWFHAKDIPGSHVLIRDNLNPSDEVKTDAAELAAYYSKARLSNLVQV
                     DMIEAKKLNKPSGTKPGFVTYTGQKTLRVTPTQGKIDSLKLKK"
     gene            21081..22085
                     /locus_tag="VBC28_000747"
     CDS             21081..22085
                     /locus_tag="VBC28_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEO6814748.1"
                     /translation="MKNKGLIATLILLTILVVGELFYNKSEKHLNLSEKQVVKIGILQ
                     YVPHDALDAIEKGVEDGLAQEGYKGKKVKLTVLNAEADQSKIQAMSKQLVNHHNDILI
                     GIATPSAQGLAASTKDTPIIMGAVSDPLGAKLVTNMKKPTTNVTGLSNVVPTKQTVQL
                     IKDITPNVKRIGILYASSEDNSVSQVTEFTKYAQKAGLEVLKYSVPSTNEIKTSMSVM
                     TKKVDAVFVPQDNTIASAFRTVIVAANQANIPVYSSVDTMVEQGSIASVAQSQYGLGL
                     ETAKQAIKVLRGKPVKDVPVKVIDTGKPSLNLKAAKHLGIKIPKKIMKQAEITVKVDD
                     "
     gene            22098..22961
                     /locus_tag="VBC28_000748"
     CDS             22098..22961
                     /locus_tag="VBC28_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6814749.1"
                     /translation="MIISSVSQGLLWGILGLGIYLTFRILKFPDMTTEGSFPLGGAVC
                     VTLMNQGVNPILATILGMLSGMLAGFVTGLLYTKGKIPTILAGILVMTSCHSIMLMVM
                     KRANLGLNEIQTLKDFLPFSNDLNLLVLGLIAILLVISALIYFLYTRLGQAYIATGDN
                     PDMAKSFGIDTDKMEMLGLIVSNGLIALSGALVSQQDGYADVSKGIGVIVIGLASIII
                     GEVLYSTGLTLFERLIAIVVGSILYQFLITAVIALGFNTNYLKLFSAIVLGICLMVPV
                     LKTKILKGVRL"
     gene            22961..23722
                     /locus_tag="VBC28_000749"
     CDS             22961..23722
                     /locus_tag="VBC28_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO6814750.1"
                     /translation="MVMKIIELKEATVQVSNGLAEMKTILDHVNLSIYEHDFITILGG
                     NGAGKSTLFNVIAGTLMLSGGNIYIMGQDVTNLPAEKRAKYLSRVFQDPKMGTAPRMT
                     VAENLLVAKFRGEKRPLVPRKITNYTEEFQKLIARTGNGLDRHLETPTGLLSGGQRQA
                     LSLLMATLKKPNLLLLDEHTAALDPRTSVSLMGLTDEFIKQDSLTALMITHHMEDALK
                     YGNRILVMKDGKIVRDLNQAQKNKMAIADYYQLFD"
     gene            24019..25680
                     /locus_tag="VBC28_000750"
     CDS             24019..25680
                     /locus_tag="VBC28_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEO6814751.1"
                     /translation="MSDIKIMALGGVRENGKNLYVVEVNDSIFVLDAGLKYPENEQLG
                     VDVVIPNLDYLIENKKRVQGIFLTHGHADAIGALPYIIAEVKAPVFGSPLTIELAKLF
                     VKNSTAVKKFNNFHVIDSETEIEFQDAVISFFKTTHSIPESMGIVIGTKEGNIVYTGD
                     FKFDQAARKYYQTDLARLAEIGRDGVLALLSDSANATSNDQVASEYEVGDEIKSVIED
                     AEGRVIVAAVASNLIRIQQVFDAAAENGRRVVLTGFDIENIVRTAIRMKRIHIADENM
                     IIKPKDMTRYEDNELLILETGRMGEPINGLQKMAIGRHRYVQIKDGDLVFIVTTPSIA
                     KEAVVARVENLIYKAGGSVKLITQNLRVSGHANGRELQLLMNLLKPKYLFPIQGEYRD
                     LSAHAGLAQEVGMSADDIYIVKRGDIMVLEKDGFFHSGFVPAGDVMIDGNAIGDVGNI
                     VLRDRKVLSEDGIFIVVITVSKKEKKIISKARVNTRGFVYVKKSRDILRESAELVNTT
                     VEDYLSKDTFDWGELKGKVRDEVSKFLFDQTKRRPAILPVVMEVR"
     gene            25737..26525
                     /locus_tag="VBC28_000751"
     CDS             25737..26525
                     /locus_tag="VBC28_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HEO6814752.1"
                     /translation="MAFFNIEYHSKVLGTERQVNVIYPDAFEISDDKIDDCDIPVLYL
                     LHGMGGNENSWQKRTNIERLLRHTNLIVVMPSTDLAWYTNTKYGLDYFDAIAIELPKV
                     LKRFFPNMSDKREKNFIAGLSMGGYGAYKIALLTNRFSHAASLSGALSFDFDLLFNNG
                     NDNINYWSGIFGDLNNTDNIERHSLRRYVESFDMKTKFYAWCGYEDFLFEANEVAIDE
                     LRQLGLTIDYFNDHGKHEWYYWNQQLEKVLEWLPIDYVKEERLS"
     gene            26615..27325
                     /locus_tag="VBC28_000752"
     CDS             26615..27325
                     /locus_tag="VBC28_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001259490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HEO6814753.1"
                     /translation="MRTLFRMIFAIPKFIFRLIWNIIWGIFKTVLVIAIILFGLYYYA
                     NHSQSEFANQLSDIIQTGKTFLNFADTNQLKNSFTNLATDSVHHSENTRWDKNQATIY
                     IATRDSELVSAYKQAISNWNATGAFVLQTTDNPNADIIAKDYSDAKTQAAGVAETEKN
                     ALTNRISKVTVKLNTFYLKNKQFGYDHTRIVNTAEHELGHALGLGHNDQQHSIMQSKG
                     SHYGIQEVDIQTLKNLYA"
     gene            27563..28234
                     /gene="rpiA"
                     /locus_tag="VBC28_000753"
     CDS             27563..28234
                     /gene="rpiA"
                     /locus_tag="VBC28_000753"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEO6814754.1"
                     /translation="MDELKKLAGVTAAKYVKNGMIVGLGTGSTAYFFVEEIGRRVKEE
                     GLQVVGVTTSNRTTEQARGLGIPLKSADDIDVIDVTVDGADEVDPDFNGIKGGGGALL
                     MEKIVATPTKEYIWVVDESKLVETLGAFKLPVEVVRYGSERLFRVFKSKGYCPSFRET
                     EGDRFITDMGNYIIDLDLKKIEDPKQLANELDHTVGVVEHGLFNSMVNKVIVAGKNGL
                     DILEK"
     gene            28291..>28967
                     /locus_tag="VBC28_000754"
     CDS             28291..>28967
                     /locus_tag="VBC28_000754"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019770913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEO6814755.1"
                     /translation="MSQFDRIHLVVLDSVGIGAAPDANDFVNAGVPDGASDTLGHISK
                     TVGLAVPNMAKIGLGNIPRPQALKTVPAEENPSGYATKLQEVSLGKDTMTGHWEIMGL
                     NITEPFDTFWNGFPEDIITKIEDFSGRKVIREANKPYSGTAVIDDFGPRQMETGELII
                     YTSADPVLQIAAHEDIIPLEELYRICEYARSITMERPALLGRIIARPYVGEPGNFTRT
                     ANRHDYAV"
BASE COUNT         9321 a         4514 c         5905 g         9227 t
ORIGIN      
        1 tttggtatat aaataagtta tttcaataat aatattgcaa attttattaa aatataataa
       61 aattaagaaa ataatggaat ttttgataaa gaatattaaa ggtgaggata aaatctatga
      121 gacctatcgt tagaaaaaag atgtacaaaa aaggtaaatt tttggtcatt gcaggtatcg
      181 ttactatttt aggtggtagt gcaattcttg gtcaagatgt caaggcagaa caggcagaag
      241 cagcaacttc aacgatctca gagaaaactg atagcagtca gactatttcg gatacttcca
      301 agttaacgtt acctgttaat tcctcggagg ctatgaaaaa tagtgcagag cctttaataa
      361 aaacaggctt tgctacatct gtttcatcta atcctagaga gatagcagct accccagtca
      421 aaacctttga tgctagttca aaagttgttg taaaagctag tactgctgag cactcagcaa
      481 atcaaacaaa ttctaatgtt aatcaagtag ctaatgatag tgaagttatt actcaacaaa
      541 attcaacaaa gcaattacca aaagtgactt actctgctca tgttcaagat attggatggc
      601 aaaagtcggt tgataatgca acagtttcag gaacagtagg acaggaaaag caggttgagg
      661 caataaaatt gtctattaaa gcccctgaag gaataacagg aaaacttagc tataaaactt
      721 atgttaaagg acaaggttgg caaccttcag tagagagtgg tcaagttagt ggtactgttg
      781 gtcagagtag acctattgaa gcactaagca ttaaccttac tgataattta caaaaattat
      841 atgatgtcta ttatcgtgtt catgttcaag atatcgggtg gatggcttgg gctaaaaatg
      901 gagcttacgc tgggactttg ggaatgtcaa agcgccttga agcttacgaa gtaaagttta
      961 cccttaaagg acaatctgtt ttaacaccaa ctatacttaa ggaagagaga cctgtcttaa
     1021 attatcaggt taaggtaggc caaaatggtt ggcaatctaa taaacttgaa ggacaaatgg
     1081 cgggaacttt aggagaatcc aaagccctag atggagtaaa attcactctt agcaccttga
     1141 agtatgggga cattctctat cgtactcatg tacaagataa aggttgggga gaaattttag
     1201 gtagtcaaac aagtaaggat tatcaaggaa aaagactaga agctattcaa ttggacttaa
     1261 gtgggaattt aaaacaaaat tatgatattt attatcgcgc tcacgtccaa gataaaggtt
     1321 ggatggcttg gcaaaaaaat aatagtgtag caggtacagt cggagaatcg aaacgtttag
     1381 aggctttgga agtaaaacta gtagctaaag cttcagattt tagttcccaa acaaatcatt
     1441 cattcttaga aatgaagcat cctggattaa gttatcaaac ttatcttcaa aaagatggtt
     1501 ggaaaccaac ggttttggaa ggccaattag gaggtagtat tggactttca aaatctataa
     1561 aagccatcaa acttaactta ggtagtacag ctctaggaaa tatagaatat agaacttttc
     1621 ttaatggttc aggttggcaa actgttgtta attcagggag agaaagtaat gtaccaaatg
     1681 aatctcaaca agtagaagct attcagatgc gattgacagg tctgctatcg aaaacctata
     1741 gtgtgatgta tcgagttcac atgcaagatt atggttggca ggattggact tataacaatg
     1801 acatcgctgg tagtactaac caaggaaaac gaattgaagc aatcgaaatt aaattagttg
     1861 agactgctaa aatacctata actgtccaaa cgacctataa gggaactgga aactataatg
     1921 tacgagtgac aaatgttatt agtcctggga atttaaaaat tgctatttgg tcagataaaa
     1981 acaatcagga tgaccttaag tggtataccg tcacacccaa aaatcatgaa gctagattga
     2041 catttaatgt tacaaatcat agagacaatg gtaaatactt tatccatgtt tatcaggaaa
     2101 atgcttctaa tcaaaaacaa ttattggtaa aaacttctct acaagtcgca cattcaaatt
     2161 ataatactcc ttactttagc caacgtgatg gaaggtgggc aagtcgaaga tatggtatgg
     2221 caactttagg agaaactggt tgtgtaccaa cttctctagc tatgattttg tcttctttga
     2281 aaggagaaac tgttttacca acgcaaattg cagattacct ttaccataag acagttgaat
     2341 ttaatcgtgg tgttcaagga acaacttcta gaggaatctt aaaagcagcc aaagaatggg
     2401 gtgtaacagc aactgcatta ggaactcaag ctaaccttgt acaagcgttg aaggatggtt
     2461 atcatgtcct agcggcagta caaaataatg tttttgttct tcatggaagt catgaaatcg
     2521 ttctcaaagg atataataac ggactgacac atgtctctga cccttacaca ccgtccttgt
     2581 ctggttggta tccaatttca caactttgga aagaacagag ctattatagt gaggatagaa
     2641 ttgatattgg agaacctttt gtcaaggtaa cagatgccta atttttaaaa ccatatgcaa
     2701 aactagcata tggttttaaa gtttccagta ctattttatt taattttatg atataatgaa
     2761 gagtaaaaca ttttaggggt ttccccttag aaagacaagt gaaatggatt taaataatta
     2821 catcgcttca attgaaaact atcctcaaga aggaattact ttccgtgata tctccccatt
     2881 aatggctgat ggtaaagcat acagctatgc tgttcgtgaa attgtacaat atgctgctga
     2941 taaagatatt gatatgattg ttggaccaga agctcgtggc tttattgttg gttgcccagt
     3001 tgcttatgct cttggtattg gttttgctcc agtccgtaaa ccaggtaaat taccacgcga
     3061 agttatttca gctgactatg aaaaagaata tggtcttgat acattgacaa tgcacgcaga
     3121 tgctattaaa ccaggtcaac gtgttttaat tgttgatgac cttttagcta ctggtggaac
     3181 agtaaaagcg acaattgaaa tgattgaaaa acttggtggc gttgtcgcag gctgtgcttt
     3241 cctagttgaa cttgatggat taaatggtcg taaagctatt gaaggttatg atactaaggt
     3301 attgatgaac tttccaggat aaggcctatt caagatagat gaatgataag ggcgcctcta
     3361 agtgctatga taatagttga gaggtgcttt ttgttacaca tttcataatt ttaggacgat
     3421 agagaggatt tgaaaggtta tgacttattt ggaacaatat cagtcagggc aattgactct
     3481 accaagtgct ttattttttc actttaaatc tatttttaag acagcagacg attttttagt
     3541 ttggcaattt ttctatttac aaaatacaac taatttatcc gatctaacac cgagccgtat
     3601 tgcgacatca cttgataaaa cagtggcaga tatcaatcgt tctatatcga atttaacaag
     3661 tcaaggttta ctagatgtca aaacaattga gttgaatcac gaaatcgaga ttatctttga
     3721 tacaagccct gtgtttgcta aattagataa acttttcgaa gaggataatc aagtaataat
     3781 agacaataaa accgctgatt cgaatcgtct gaaagattta gtaggagact tcgaacgtga
     3841 attaggtcgc ttattaagtc cttttgaatt agaagattta caaaagacac tgcaagaaga
     3901 tcagacagat cctgatattg ttagggcagc tcttagagaa gcggttttca atgggaaaac
     3961 aagttggaat tatatcaatg ctatccttcg caactggcgg cgtgaaggat tgacgacact
     4021 tcgacaaatt gaagagagaa aacaagcacg tgaagataac caaatgaaag atttagcgat
     4081 ttcggatgat tttaaaaatg caatgaattt atgggaataa gtttactagg aggagacaga
     4141 atggttgata cattaggtct gttccttttc ttttgatgtt aatgatgtta tacttttaac
     4201 agagaaaaac tgaaggaaat acaatggatt tacaattatc aaaacgatta caaaaagttg
     4261 caaactatgt tccaaaagga gctcggttat tagatgtagg aagtgatcat gcttacttac
     4321 ctattttttt gttgcagatg ggatactgtg attttgccat tgctggtgaa gtggtgaatg
     4381 gaccgtatca gtctgcgttg aaaaacgttt cagaacatgg tctcacttca aagatagatg
     4441 ttcgcttagc aaatggtttg tcagcttttg aagaagcaga taatattgat acgatagcta
     4501 tctgtggaat gggtggtcgt ttgattgctg atattttgaa taacgatata gacaaacttc
     4561 agcacgttaa aacactagtc ttacaaccta ataatcggga agatgattta agaaaatggt
     4621 tagctgcaaa tgattttgaa atagtagcag aagatatctt gactgaaaat gacaaatgct
     4681 atgaaatttt agttgttaag catggtcata tgaatttaac tgctaaagaa ttgcgatttg
     4741 gtccattttt attatccaat aacacaacag tttttaaaga aaaatggcaa aatgagctta
     4801 ataaattaac ttttgcttta aactctattc ccaatagtaa aatggaagaa cgtgctattt
     4861 tggaagataa aatacaggat ataaaggagg ttcttgatga aagctagaga acttattgat
     4921 gtatatgaaa cttattgtcc acaggaatta tctatggaag gagatatttc aggtcttcaa
     4981 attggatctt tagataaaga aataaagaca gtcatggtag ctttagatgt tcgagagact
     5041 acagtagctg aagcaattga acgtcaagta gatcttctta ttgtaaagca tgctcctatt
     5101 tttcgtccat taaaggattt ggtagcaaca ccgcaaaata agatttatat agaccttctt
     5161 aaatcagaca ttgctgttta tgtgagccat actaatatag atatcgttcc taatggactt
     5221 aacgactggt tttgtgaatt attagatatt caatatcccg atatattatc tgagacaagt
     5281 aatggttatg gtattggtcg cattggtgat gttagacccc agagttttga attttttgca
     5341 tggaaaatta aagatgtgtt tggtttagat agcgttcgtt tagtttcata tgataaatct
     5401 aatcctgaaa ttcaacgagt tgctatttgt gggggtagtg ggcaaagttt ctacaaagaa
     5461 gctattgcaa aaggagcaga tgtttttgta acgggagata tttattatca tactgcacaa
     5521 gaaatgatca caaatggttt gcttgcgatt gacccaggcc atcatattga ggttttattt
     5581 gtttctaaaa tagctactat gattgagcag tggaaacttg aaaaaggctg ggatatttca
     5641 gttttagaga gtaaagctcc aacaaatccc ttttaccata tgtaggagct attatgaaaa
     5701 agatagcaat tattggtgca ggagcagtcg gagcgacact agcctattac ttatcaaaag
     5761 aaaaggatat tcaggttact gtttttgatt atggagttgg acaagcaact aaggctgctg
     5821 cggggattat tagcccatgg ttttcaaaac gtcgtaataa agcctggtat cgcatggcga
     5881 ggttgggggc tgatttttat tcaaagctag tcacggattt acaaaaagat ggttttgaaa
     5941 caaaatttta tcagcaaact ggtgttttct tattaaaaaa ggacgagagt cagttagagt
     6001 cattgtttgc actagcagat aaacgtcgac tagaatctcc tctaataggt gatttacaaa
     6061 tactaaataa aagtgaagca aatactcact ttcctgagtt agatggctat gaacagttac
     6121 tttatgcgag tggaggatcg cgagtagaag gggcggactt aacacgaata ttgttagaag
     6181 caagtggtgt taatgtcatc aaggatgaag ttcactttac gataactgat aatggctttc
     6241 gtgttcaagg aattgatttt gataaacttg ttttagcgag tggagcctgg ctagctaaga
     6301 tattagatga gcacaactat caggtagata ttcgccctca aaaagggcaa ttgcgagact
     6361 attattttag caatattaat acaggaaaat atcctgttgt aatgccagaa ggcgagctag
     6421 atattatccc atttgataat ggtaaagtct ctgtaggtgc aagtcacgaa aatgatatgg
     6481 cttttgattt aaacattgat tttaaagtct tagacaagtt tgaagagcaa gctattggct
     6541 attttccgca attaaaaaaa gcagatacga cttctgaacg tgttggaatt cgtgcctata
     6601 caagtgattt ttctccattt tttggccctg tcccttgtat ggagggtgca tatgctgcaa
     6661 gtggattagg atcaactggc ttaacagtgg gcccattaat tggttatgaa ttatgtcaat
     6721 taatcttaaa taaagaaaat cagctaaatt tagaggatta cgatattacg aaatacgtga
     6781 aaaagattag tagatgatag cgtttaaaaa aagtgttata atatgtagga ttaatataaa
     6841 taaggagacc ttactatgaa aggtattatt ttagcgggag gttcaggtac tcgcttgtac
     6901 ccattaactc gtgctgcatc aaaacaattg atgccaattt atgataaacc aatgatttat
     6961 tatccattgt cagttttgat gttggcagga ataaaagaaa tactgattat ttctactcca
     7021 caagatttgc ctcgctttga agatatgctt ggagatggtt cagaactagg aatctctctt
     7081 tcttatgctg aacaaccaag cccagatggg ttagcacagg ccttcattat cggtgaagaa
     7141 tttattggcg atgatcacgt tgccttagta ttaggtgata atatttacca tggaccaggt
     7201 ttatctgcaa tgctacaacg tgcagctagt aaagaaagtg gagcaaccgt ctttggctat
     7261 caagtaaaag atccagaacg ttttggtgtt gtcgagtttg atactgatat gaatgccatt
     7321 tctattgaag aaaaaccagc gcaacctaag tctaattatg ctgtcacagg tctttatttc
     7381 tatgataacg atgtggttga aatagctaaa aatattaagc caagtcctcg tggagaactt
     7441 gaaattactg atgttaacaa agcctacctt gatagaggtg atttatcagt tgagttaatg
     7501 ggacgtggat ttgcgtggtt agacactgga actcacgaaa gtcttcttga agcagcacaa
     7561 tatattgaaa cagtacaacg catgcaaaac gttcaagttg ctaaccttga ggaaattgct
     7621 taccgcatgg gctacatcac tcgtgaacaa gtccttgagt tggcacaacc acttaagaaa
     7681 aatgagtatg gtcaatacct acttcgtttg attggagaag cctaatgact gaacaatttt
     7741 ttgataaaga attaacttgt cgcccaattg aagccattcc tggattgttg gaattcgata
     7801 ttaccgttcg tggagacaac cgtggatggt tcaaagagaa ctttcaaaaa gaaaaaatga
     7861 taccgcttgg tttcccagaa agcttctttg aggcagacaa actacaaaat aatatttcgt
     7921 ttaacaaaaa aaatacattg cgaggtctcc atgcagagcc ttgggataaa tatgtttcga
     7981 tcgctgatga aggacgtgtg atcggtactt gggttgacct ccgtgaaggt gacagttttg
     8041 gtaacgttta ccaaacgatt atcgatgcct caaaaggtat ctttgttcca cgcggcgttg
     8101 ctaatggttt ccaagttctt tcagataaag cagcttatac ttatctcgtt aacgattatt
     8161 gggcacttga actcaaacca aaatatgctt tcgttaacta tgcagatcca aatctaggca
     8221 ttcagtggga aaaccttgaa gaagcagaag tctcagaagc agataagaat cacccacttc
     8281 tcaaagatgt aaaacctttg aataaggaag atttgtaatt ttcaacttct taaaaggcat
     8341 taaccttagc aagtaaaaac gaattagcaa tgttgtttga cgcaagggtg aaaggaaaca
     8401 tgcaaaaggc ctgttccctc agggatttga aatattgatt tcttaggcat gacattccat
     8461 agaactactt ctaacagaac agtatccttc aaacagcaaa aagaaaaaat ttttcggaga
     8521 aaaaatgact gaatttaaaa acattatcgt aactggtgga gctggtttca tcggatcaaa
     8581 ctttgtacac tatgtttaca ataaccaccc atatgttcat gtaactgtcc ttgataaatt
     8641 gacatatgca ggtaaccgtg ctaacattga agccattctt ggtgatcgtg ttgagttagt
     8701 tgttggtgac atcgctgacg ctgaattggt agacaaattg gctgccaaag cggatgctat
     8761 tgttcactat gcggctgaga gtcacaacga taactcattg gaagatccaa gtccatttat
     8821 ccatacaaac tttatcggaa cttacacttt gcttgaagca gctcgtaaat acgatatccg
     8881 tttccaccac gtgtcaactg atgaagttta tggagatctt ccacttcgtg aagaccttcc
     8941 aggaaatggt gaaggaccag gtgaaaaatt cactgctgaa acaaaataca atccatcatc
     9001 accttactca tcaactaagg cagcttctga ccttatcgtt aaggcatggg tacgttcctt
     9061 cggcgtaaaa gcgaccattt cgaactgttc aaataactat ggaccatacc aacacatcga
     9121 aaaattcatt ccacgtcaaa ttacaaatat cttggcagga atcaaaccaa aactttatgg
     9181 tgaaggtaaa aacgtccgtg actggatcca cacaaatgac cattcaacag gtgtctgggc
     9241 tattttgact aagggtcgta ttggtgaaac ataccttatt ggtgccgacg gtgagaaaaa
     9301 caacaaggag gttcttgaac ttatccttga gaaaatgggt caaccaaaag acgcttacga
     9361 tcacgtaact gaccgtgctg gtcatgatct tcgttacgct atcgattcta caaaattgcg
     9421 tgaagaactc ggctgggaac cacaatttac aaacttttca gaaggtttgg aagaaactat
     9481 taaatggtat acagaaaatg agacatggtg gaaagcagaa aaagatgctg tagaagccaa
     9541 gtatgctaaa actcaagaag tcattaagta attgaaccaa aaaacctatc attttgatga
     9601 taggtttttt atgtacggct agttagatag ctaattggtc tgtttttgtc atggtttgag
     9661 gttggtaata gacattttga tagtcattac caactttttg tactaggtaa agtcccgctt
     9721 tattcatttt gaattgattg ttaatgagac tctcttgatt atcgtgtttg ataacagccc
     9781 aagtttgact gtttttatca tagataactt gttgttttga actattttct agtaattcta
     9841 cttctttgct gttggctaat ttatcaaaac ttgtgcgatc aatgtttgga accatcatgt
     9901 aaccatagct atcacctttg ttgtcatgct tttggcttat agtgataaaa ggattgctaa
     9961 cgattgaggt atttttagaa gtacgattaa tgctgttcca agtacctgtt tgctctttgc
    10021 gttcaatatc aatagtgcta tttttaaaga agatataacc aatattgcga ccaggttctt
    10081 ttgattctaa aaagacactt tttgtatctg taaattgttg agaacttgct tgttttaaat
    10141 caatagtttt tccattgacg tatgtagtat aaggtgtttt agaatcgtct tttcgttgat
    10201 caattgttgt agaaacattt ccaatgccgt tagtattctt gatgttgcta cctaaaaaga
    10261 caatcttatc atttaagata acccaacctt tttgtgctgt taaggtgcga tcccagttag
    10321 taaaatccat agcggcaaga gcaaagtgat cattaagttt gacggaacca acaaagtcag
    10381 atgttcctgt aacttgaccg gttttttctt tagcgtcttt gctatgtttg ctcatgaatt
    10441 ccttagtggt atcttcacgc ttagcatctt tttcagttgt tccagccatt ttataaggat
    10501 tgacggttgg ccaaaaatga ttactataat gagattgatc actattataa agatagaaca
    10561 taccatctcc ggtataccaa ccacgtgtat tttcatcatt cattccttca tagttgaggg
    10621 tacgtttaga atgtaatgaa agcgcgaaac caaagtcttt ctcggcatta taataagcta
    10681 agcggtccat gctattaaag gttgataaat tactttttaa aggtttagta gcgactgtac
    10741 tgtcattaag catcttattc atgttggcaa tatcggaata cgatttgagg ttattgaaga
    10801 cattgtagaa tttatttgaa gtgataatcg ttttaatagt tgatttgagg tctaagtttc
    10861 gctcttcatt agacatgtta gccaatctga ggaaacctct gagaacttca actgcagccg
    10921 catgcgaaga agctgcctct ctactaattg agcgtccacg actcatatcc attaactcac
    10981 cttttacaat taaaggtaaa aatgattgat taatccattt ataaaccata tcaagttctt
    11041 gattagagat tttatagtca gtttcttgaa tgataggcag caattgtgtc aaaccatcta
    11101 tcagaacatt accataggcg ccagtataag caacatttgt atggtcgatg taagaaccgt
    11161 cagcatagaa accttcagct ttagtagcag tagtaaaaag attttttaga gaatgagaag
    11221 ttttttcgat aatagtattg tctttacgaa gtaaaccttc aatgatttta acgcgcccca
    11281 tatcgactag attaccacca agggctttaa atggattgtc aagcgtttta cggaaatatc
    11341 ctgcatcagg aacaaagtgt tcaattgggt cggtataagt ttttatttca gcgtcagtga
    11401 agtagttatt catgagagct agggtagctg taattgagcg agggacaccg atttcaaaat
    11461 cccaccaatt ggcagagcct tctatatctt tattaacatt gtagaagttt tgatgaagcc
    11521 aagccagact ctcttttaca gtacgaatag ctttttcatt tttgtaaata gtagagtggg
    11581 gattggtgat ttgtttagct aaatcttcca aacgacgata agtagcggtt aactgtgctg
    11641 aattattgag attatctaaa tctttccaaa ggaaagtgtg attagaatcc agtttgatag
    11701 ttttgatgtt tttggcgtta gtttcatcta atttctgatt aatcttttgc atgttcgaat
    11761 cattagtatc gtaaacatga ttaccaatag tcacgtcgtt ccatttgtcg aggagttttg
    11821 taaacttatc ttctgttgaa gctgtaactg ataacggtac ttctttaatt attttaccag
    11881 atttatcaga tattttaaca gttgttgaac cagtagcgtt tggatacaat attccaccaa
    11941 caattttccc gagagacgga ttggttagag tgtattggta gtcagcttta ttaaaaacgt
    12001 aatttttgtt taaagccgta ttaacgcttt cttcaatttg tgttgtgacg ggttgtggat
    12061 gctctgagtc tttagggcct tttgctttca ttgatatatt atcaaaagta gcagaaccag
    12121 ttcctttttc ataataaagt tcaagtgtca cctcggagac atttaacttt gggttatata
    12181 tctttgttaa ggtatgtttg ttagtagtac cgctggtcat ctcagaaagc caaagtcgcg
    12241 tattgttgtc ttttttctcc ataatacgaa ggaaagcttg tccagccttg ttacttgttt
    12301 caatatcaaa cttgacctca tattttttag tgggatcaat gttcactttt tgtgttacag
    12361 cacctctaaa gcctttgtcg ctggataggc ttacttggcc ttgttcggta cgttggataa
    12421 taggtgattt atcagcagta ttttctttat caatccaagc agaccattcc ctagcgcttg
    12481 tatgtgacca gcttccagat acagggttag tttgattaaa atctccgttt ttcaccagct
    12541 ccttattttg agtttgatta tctactactt gtttcattgt gctatgagtg gctgttgcag
    12601 ttgcggttga agctgtgggg ttagacagat cttgttgtgt ggttgtggtg ctagtctctt
    12661 tgacagtggt actttcagtc tggagaactt caatattatt agtttgagta tctcttattt
    12721 gggacgttga ggtagttttg gtaagctctt cagctttagc ttggtaacta ttgttaacca
    12781 atattgttcc taaaataagg gctgaataaa gcataatacg atgtttcttt ttgatttcca
    12841 tttttactcc ttagggttta aaattgtaaa ccggtttttc ctaattatag catgctttaa
    12901 aggtttatga taaagaacct ttaaaatgtc atcaaagtga aaagtaaata actggtaaac
    12961 aaaataattt gtttaatcat ttgtgtgcta ctttgatagt aaattgccta aataaaggct
    13021 attttttgtt ataatatgag aatgagagga gtttgaaatg actaaattag caacaatttg
    13081 ttacattgac aatggcaagg aattacttct attgcaccgc aacaaaaaag agaatgatgt
    13141 tcatgaggga aagtggatct cggtaggcgg taaactggag gcaggtgaaa ctccggatga
    13201 atgtgctaag cgtgaaattc tagaagaaac ccatttaaca gtaaaaaaaa tggattttaa
    13261 aggagtcatc acttttcctg aatttacgcc aggtcatgat tggtatacct atgtctttaa
    13321 agtaacagat tatgaaggag agttaatttc agatgatgaa tcacgagaag gaaccttaga
    13381 atgggtacca tatgatcaag ttctctctaa gccaacgtgg caaggagatt acgaaatttt
    13441 taaatggatt ttagaagatg ttcctttctt ttctgctaag tttgtttatg atgaacacca
    13501 aaatttaatt gaaaagacgg ttaattttta tgaaaaatag agattttagg gttagagacc
    13561 agctcgaaac attcggagtc agagtttctg ctttaatcat agaaaatcaa aaactgctat
    13621 taatatatgc tcctcatctc gataaatact atttgccagg tggggcgctt caagttggag
    13681 aagatagcaa taaggctgtt gcgagagaag tacttgagga aataggtctt cacagccaag
    13741 ttggtgattt agcctatatt gttgagaatc agtttaatat taagagacat cattatcact
    13801 cagttgaatt tttatatttt gttaatctct tgggtcaggc gccagaatct atcaaagaag
    13861 gaacgcataa gcgacatttt gtatggcttc caataaaaga attaacaaaa attgattgta
    13921 atcctaattt tttagcacag gatttgatag aatggccagg tcatgttgtg cataaaatcg
    13981 ttcaaaatta actttttaca ctcttgtttt gagtttaatt taatttaacg tgttataata
    14041 aatgagaatt aaaattagaa ttcatcttga aaggatatgt gaatgaatcg accatcagaa
    14101 aaagaattta aaaatagttt gttttttaaa tggatcttaa ataatcaagc agttattgct
    14161 ctcatgatta cctttttggt atttttaacg atttttattt ttaccaaaat ctcttttatg
    14221 tttaaacctg tgtttgattt tcttgctgtg ctgatattgc cgcttgtaat ttctggattg
    14281 ctttattacc tattaaaacc tatggttaca tttttagaga agcggggaat taagcgtgta
    14341 acagcgatat tatcagtttt tactattata atccttctgt taatttgggc aatgtctagt
    14401 tttattccca tgatgagtaa tcaattacgc cattttatgg aagatctccc ttcatatgtg
    14461 aataaagtgc aaatggaaac aagttcgttt atagatcaca acccttggtt aaaatcttat
    14521 aaaggggaaa tatcgagcat gttatctaat atcagtagcc aagcggtctc ttatgctgaa
    14581 aaattttcaa agaatgtttt agagtgggca ggaaatttag ctagtacagt tgcacgtgtg
    14641 acagtagcaa caatcatggc tccctttatt ttgttttatc ttttaagaga tagtcgcaac
    14701 atgaagaatg gtttcttaat ggttttacca accaaactac gccaaccaac tgatcgtatt
    14761 ttgcgagaaa tgaatagtca aatgtcagga tatgtgcaag gacaaatcat tgttgctatt
    14821 actgttggta ttattttttc aataatgtat agtattatag gccttagata tggcgtgaca
    14881 ttagggatta ttgccggtgt gttaaatatg gttccctatt tgggaagttt tgtcgcccaa
    14941 attccagtgt ttatcttagc gcttgtcgca ggacctgtta tggttgttaa agttgcgatt
    15001 gtttttgtta ttgagcaaac tctagaggga cgctttgtct cacccttggt tttaggtaat
    15061 aaacttagca ttcatccaat tacaattatg tttattttat taacctctgg agcgatgttt
    15121 ggtgtttggg gagtattcct cagtattccg atttatgcat ctatcaaagt tgttgttaaa
    15181 gaattgtttg attggtacaa agctgtcagt gggctatata cagtagatgt tgttactgaa
    15241 gaaagaagtg aagaagttaa aaatgttgaa tagtgaaaaa atgattgttt ctatccaaaa
    15301 tcaggattta gaacatgcta acaagtattt cgaaaaagcg ttgaaaaatg atcctgagga
    15361 agtcttattg gaactaggag cttatttgga gagtattggt tttttacccc aagcaaaacg
    15421 tctctatgat caaatccgac caaattatcc agaagttgct atcaatttag ctcaaattgt
    15481 tgccgaagat ggagatattg aacaagcatt cttatactta gattatatct cagaagacag
    15541 tcaagagtat gtttcagccc tccttgttat ggctgactta tatgatatgg aaggcttgac
    15601 tgatgttgcg cgtgaaaagc ttttgctagc cagcaaactt agcgatgacc cattggttac
    15661 atttggttta gctgaaatga accttagttt ggaacattat caagaagcga tagaaggata
    15721 tgcttcactt gacaatcgag aaatcttaga aacgactggg gtttcaactt atcagcgtat
    15781 tggtaaatcc tacgctatta tggggaaatt tgatgcagct atcgaatttt tagaaaaagc
    15841 cgttgacatt gagtatgatg atttaacagt ttttgagtta gcaacaatcc tttatgatca
    15901 agaagaatat caaaaagcta acctttattt taaacagttg gatactatta atccagactt
    15961 tgatggctat gaatatatct atggtttatc tcttcgtgag gagcataaat ctgaagaagc
    16021 tttacgtcta gttcagcaag gtcttcgtaa aaatagtttt gatggtcaac ttctcttact
    16081 agcctcacaa ttatcgtatg aattacatga tgtttcttcg tcggagtctt atttgaaaca
    16141 agctgaaaaa gtttctgaaa accaagatga aattgttatg agattgtcaa atttgtatct
    16201 tgaagaagag cggtttgagg aagtcttgga attagacaat gatgatttag aaaatatcct
    16261 tgcaaaatgg aatatcgcta aagcacacaa ggctttagaa atggatgata gtgtagacta
    16321 ttatcaatct ttgtataatg atttaaaaga taatccagaa tttttacaag attatgctta
    16381 cattttgaga gaatttggtt accttgataa ggcacaagaa gtaggaaagg cttatttaaa
    16441 attagttcca gatgatatag agatgtcaga atgggtgaat aacatttaaa taggaaaaca
    16501 cttacagaaa aaaataaaaa attcacaagt aaattttcac aatctgatgt aaaaactgtg
    16561 atataattga accattcttg tgaaaagagg gagataaaat gacagaatca cataatcaat
    16621 atggggctga cttaattgtc gatagcctca ttaatcatga tgtcaaatat gtttttggta
    16681 tcccaggagc taagattgac cgcgtatttg acactctaga agataaaggt ccagagttaa
    16741 ttgttgctcg tcatgagcag aacgcgactt ttatggctca ggctgtcggg cgtattacgg
    16801 gagaaccagg agttgttatt gcaactagtg gacctgggat ctcgaacttg gcaacagggt
    16861 tagttactgc gacagatgaa ggtgatgctg ttcttgcaat tggtggacag gttaagcgag
    16921 gagatttatt gaaacgtgct catcaatcaa tgaataacgt tgctatgcta gaacctatta
    16981 caaaatattc tgctgaggtt catgacccta atactttgtc agaaactgtt gcaaacgcct
    17041 atcgtcttgc taaatctggt aaaccaggag caagttttat ctctattcct caagacgtta
    17101 cagacagtcc agtctcagta aaagcaatca aacctcttag tgcacctaaa ttgggttcgg
    17161 catctgtttt agatattaat tatttagcgc aagctattaa caatgctgtt ttaccggtac
    17221 tgttattagg aaatggcgca tcttcagagg gagtaactgc tgccgtacgt cgacttcttg
    17281 atgctgttaa actaccagta gtagaaactt tccaaggtgc gggtattgtg tcacgtgagc
    17341 tcgaggatga aacattcttt ggtcgtgtag gcctcttccg taatcaacct ggagatatgc
    17401 ttttgaagcg tgcagaccta gttattgcta tcgggtatga ccctattgaa tatgaagcgc
    17461 gtaactggaa tgctgaaatt tctgctcgta ttattgttat tgacgttgaa caagcagaaa
    17521 ttgatacgta tttccaaccc gagcgagaac tgattggaga catggcacat acactttatt
    17581 tacttttacc agctattaaa ggatatgaat tgcctgaagg ttcaaaagag tatttgaaag
    17641 ggcttcgtaa taacattgaa aatgttagcg atgttaaatt tgatcgtgac tcagctcatg
    17701 gacttgttca tcctcttgat ctcattgatg tactccaaga aaataccacc gatgatatga
    17761 ctgttacagt tgatgtgggg agtcattata tttggatggc tcgctacttt aaatcatatg
    17821 aagcgcgtca cttgctcttc tcaaacggaa tgcaaacatt aggagttgct ctaccttggg
    17881 caatttctgc ggctcttttg cgtcccaaca caaaagttat ttcagtttct ggtgatggtg
    17941 gcttcttatt ctcagctcaa gagttagaaa ctgcagttcg tcttcatcta ccaattgttc
    18001 acattatttg gaacgatgga aaatataaca tggttgaatt ccaggaagaa atgaagtatg
    18061 ggcgttcttc aggtgttgac tttggaccag ttgattttgt aaaatacgct gaaagtttcg
    18121 gtgctaaagg ttaccgtgta gacagcaaag acagttttga agaaacactc aaacaagctc
    18181 taatcgatgc tgaaaatgga ccagtgttga ttgatgtgcc tatcgactat aaagataacg
    18241 tgacactagg tgaaacaatc ttacctgacg agttctacta agacgaggtc taatatgtca
    18301 gaaacagtta aattatttca atacagcacc ttgagttcac tgatggcagg attgtacaaa
    18361 ggcagtctaa ctatcggaga attattaact cacggtgact taggaatcgg tactgttcat
    18421 atgattgatg gtgaattgat tgtattggat gggaaagctt atcaagctat tggtacagat
    18481 ggtaaagcag agattatcca attatcagat gatgtgacag taccctatgc agcggtttta
    18541 ccacactata tccaaaaaca atttgatatc aatgctgaga cagataataa ggatttggaa
    18601 gagatgattc taaaaaactt tgaaggtcaa aatctcttta agtctttaaa aatcaaagga
    18661 acgttttcaa gaatgcacgt tcgtatgatt ccaaaatctc tacaacataa acgttttgca
    18721 gatattgcat ctaatcagcc agaatttacc agagagaatg tatctggtac gttggttggg
    18781 atttggacgc cggaattatt tcatggtgtg ggggtaaaag ggttccatgt ccactttatt
    18841 tcagacgatt taactttcgg tgggcatgtt atggattact cgctcacaca aggaaaagtt
    18901 gaaattggga aggttgacca acttgaccaa tgtttcccaa ctcaagatca agaatttttg
    18961 gaagctaact ttgacctaca aaagcttaga gaagatattg atttaagtga ataagaatac
    19021 ctataaataa ttaataacac taaaaataga cagggatatg tcccagtcta tttttttagt
    19081 ttaaggctat ctatttttcc ttgtgtggga gtgactcgta aggttttttg accagtgtaa
    19141 gttacaaaac ctggcttagt accacttggt ttattgagtt tttttgcttc aatcatgtct
    19201 acttgaacaa gattggagag acgagcttta gagtaataag cagctaactc ggcggcatct
    19261 gtcttaacct cgtcacttgg attgaggtta tcccgaatta acacatgact tccggggata
    19321 tccttggcat gaaaccacaa ctccccttta cgagccattt taaatgttaa ctcgtcattt
    19381 tggagattat tacgtcccac cataataata gtcttgccat cggatgctaa atattgttca
    19441 ggttttttgc gcttatgttg tttatcatgt gcgcgtcgct taataaatcc agtttcaaca
    19501 agttcttcac gaatatcatt gatatcttcc atagaagcat gatttagcga tgtttcaaca
    19561 gattcaaggt aggtgattgt attttccgta tctgaaataa ttccttttaa atgttttaca
    19621 gcttctttta atttttgata ttttttaaag tagcgttggg cattttggtt gggtgttaaa
    19681 gctcgatcaa gtgaaatctc aatggtttgg ttggtgtaat aattatcaag cacaacgaca
    19741 tcttgattat ttggtaccat agagagatag gtagttagaa gctctccttt ttggcgaaac
    19801 tcctctgcat tttcagtagc tagaagttcg tcttgttgct tagctagttt tttgatattt
    19861 ttctctaact cactttgaac acggtggatg aggtcactag cttgctgtgc tatgcgatct
    19921 ttttcagcct tctcttggta gtaataatct aacaattgtc caagagactg aaatgtttcg
    19981 tgactgcttg agaattggac agcggaaaag gatttttctg ttaggctggg ataatattct
    20041 ctcgaaaaga attgacgaaa atcgttaagt ttattgtctt tcaaacagtg agataattct
    20101 agcgcagtat cgcgtcctaa accttggaag agctgttgga ggtttttagg gcttagatca
    20161 ttggtttgaa gcagctcaaa tagagtttga tcagaaatat caaaaggatt gattgctttt
    20221 gttttgggtg gggcaatgta agtcgaacca ggaaggatag tacgatagga gttttgtgag
    20281 aaaccaacat gcttaattga ttcaataatt ttatgttcat ttttatcaat caagataatg
    20341 ttactgtgtt ttcccatgat ttcaacaaca agtgttgctt taatgtgatc accgatttcg
    20401 tttttattag acacgatgat ttctaatata cggtcatttt caatttgttg aatggtttca
    20461 atcactgcac cttgcaaata ctttctcatg atcattgtaa aagtatttgg attttgagga
    20521 ttttggaaat ttgcttcggt agtttggata cgtccaaata cagggtgagc agataggagt
    20581 agtttatagt tgcgacgatt attgcgtatt gttaatacca attcatggtc aaaaggttgg
    20641 ttaacttttt ggatacgtcc tttttcaatc tgttcttgta attcatttgt gaggtgatgt
    20701 aaaaaaaatc catcaaaaga catggcgtcc cccttattct aaaaattata gtttatttat
    20761 tataccatag gctgagctaa aaatattttt tataacgcaa aaggcttgca attttcagaa
    20821 tatagtatat aattatagaa aaattcatga gaaaggatga ctagtaaatg actaaaacat
    20881 tatttaccaa ttggcaaaac tggcgtagat aaagttgtgt ttatgggtgc atagatacga
    20941 ctttttggag tacttcaaaa aagtatctat gcgctggtct agtctaacaa tagagagcgc
    21001 aaggtaagtt atttatcagg cgctttttct tttgcgctaa actccagaaa gtcagagtat
    21061 tattaaagtg aggagaaaat atgaaaaata aaggactgat agctacgcta attctattga
    21121 ccatattagt ggttggggaa ttattttata acaaatcaga aaaacatctt aatttatcag
    21181 aaaagcaagt tgttaaaatt ggtattttac agtatgtacc ccatgatgct ttggatgcta
    21241 ttgaaaaggg agttgaagat ggtctagctc aagaaggata taaagggaaa aaagttaaat
    21301 taaccgtttt aaacgccgaa gctgatcaaa gtaaaattca agcgatgagt aaacaattag
    21361 ttaatcatca caatgatatt ttgattggaa ttgcgacacc atcagcacaa ggtttagcag
    21421 cttcgactaa ggatactcct ataatcatgg gagctgtttc tgatccttta ggggccaagt
    21481 tagtaacgaa tatgaagaaa ccaacaacta atgttacagg attatcaaac gttgttccga
    21541 ccaagcaaac tgtgcaatta atcaaggata taactcccaa tgttaaacgt ataggaattc
    21601 tttatgctag tagcgaagat aattctgtgt cacaagtaac tgagtttaca aaatatgctc
    21661 aaaaagcagg actagaagtt cttaaatatt ctgtaccatc tacaaatgaa attaagacaa
    21721 gtatgtcagt catgaccaaa aaagtagacg ctgtctttgt tccacaagat aatactattg
    21781 cgagtgcttt tagaacagtt atcgtagcag caaaccaagc taatatacct gtgtattcca
    21841 gtgttgatac tatggttgaa caaggaagta tagcatcagt agcgcagagt caatatggct
    21901 tagggttaga aactgcaaaa caggccatta aagtcctaag agggaagccg gttaaagatg
    21961 taccagtcaa agttattgat acagggaagc caagcctcaa tttgaaagca gccaaacact
    22021 taggaattaa aattcctaag aagattatga aacaggcaga aattaccgtt aaggttgacg
    22081 attagaaaga gggttttatg attatttcat cagtttcaca aggtttatta tggggaatat
    22141 taggattagg gatatactta acctttagaa tcttaaaatt tccagacatg acaacagagg
    22201 gctccttccc tctaggggga gcggtttgtg ttaccttaat gaatcagggg gttaatccta
    22261 ttttggcgac gattctaggc atgctatcag ggatgttagc gggatttgtt actggtttac
    22321 tttatactaa aggaaagata ccgacgattt tagcaggaat tttagtaatg acctcatgcc
    22381 actccattat gctaatggtt atgaaaagag caaatctagg tttaaatgag attcaaacat
    22441 tgaaagattt tcttccgttt tcaaatgatt taaatcttct cgtattaggt ttgatagcta
    22501 ttctcttggt gatttctgcc ttaatttatt ttctctatac acgcttgggg caagcttata
    22561 ttgcaacagg agacaaccct gatatggcaa agagcttcgg cattgatacc gacaaaatgg
    22621 aaatgcttgg cttaattgta tcaaatggct taattgcttt atcaggagct ttagtaagtc
    22681 aacaagatgg atatgctgat gtatcaaaag gtataggtgt tattgttatt gggttagcta
    22741 gtattattat tggagaggta ctttactcta ctggtttaac tctctttgaa cgtttaattg
    22801 ctatagtggt gggttctatt ctttatcaat ttttaataac ggcagtaatc gccttaggat
    22861 ttaatacgaa ttacctaaaa ttatttagtg caatagtgct tggaatttgt ttaatggttc
    22921 ctgtcctaaa aacgaagatt ttgaaaggag tacgcttata atggtgatga aaatcataga
    22981 gttaaaagaa gcaaccgtac aagtcagtaa tggtttagca gaaatgaaaa cgatattaga
    23041 ccatgttaat ttgagtattt atgaacatga cttcattaca atattgggtg gaaatggagc
    23101 tggaaaatca acgcttttta atgtaattgc aggtaccttg atgttaagcg gtggaaatat
    23161 ttacatcatg ggacaagatg ttactaattt accagcagaa aaacgagcta aatatttatc
    23221 acgggtattt caagatccga aaatgggaac agcacctagg atgacagttg ctgaaaattt
    23281 attagttgct aaatttcgag gtgaaaagag accgttagtt cctagaaaga ttactaacta
    23341 taccgaagaa tttcaaaaat tgattgctag aactggtaat ggacttgatc gccatttaga
    23401 gacacctaca ggtttattat caggtggaca aagacaagca cttagtttat taatggcaac
    23461 tttgaaaaaa ccaaatctgt tattattgga cgaacacaca gcagcgcttg atcctagaac
    23521 aagtgtttcg cttatgggct tgactgatga gtttatcaaa caagattcgt tgactgcctt
    23581 aatgattaca catcatatgg aagatgctct caaatatgga aatcgtatat tagtaatgaa
    23641 agatggcaag attgtcagag atttaaatca ggcacaaaaa aacaagatgg ctatagctga
    23701 ctattatcaa ttatttgatt aaaaagaagt gaaatgggag tgtttcactt ctttttctat
    23761 acactttgca agaaattatt ttcaaaatat gatatactaa taatgctgta aatcgacttg
    23821 tagagacaag gaccgtaagt acaaatggaa tgaaagtaag gacacactct ttaattcata
    23881 ttcctattat tgattaataa ggtttttctt caaacccaag aaaagttacc gataaatcaa
    23941 ttactaatga taataattgg acaatttctt gtctgcttta taaaaaagaa ttaacaaaat
    24001 ttaaggagaa aactcattat gagcgacata aaaatcatgg ctcttggagg tgttcgtgaa
    24061 aacggaaaaa acctatatgt agtagaagtc aatgattcta tctttgtttt ggatgctgga
    24121 ctcaaatatc ctgaaaatga acaactgggt gttgatgttg ttattccaaa tcttgattac
    24181 ttaattgaaa ataaaaaacg tgttcagggg attttcttaa cccatggaca tgcggatgct
    24241 attggtgcat tgccttatat tatcgccgaa gtcaaagcac ctgtgtttgg ttcacctctc
    24301 acaattgaat tagctaagtt attcgttaaa aatagtacag cagtcaagaa attcaataat
    24361 ttccatgtga ttgatagtga aacggaaatt gaatttcaag atgcggtgat ttcgttcttt
    24421 aaaacgactc attcaattcc agagtcaatg ggaatcgtta ttgggacaaa agagggaaat
    24481 atcgtttaca caggtgactt taaatttgat caagctgcaa ggaaatatta ccaaacagat
    24541 ctggcacgtt tagctgaaat tggtcgtgat ggtgttttag cacttttatc agattctgca
    24601 aatgctacaa gcaatgacca agttgctagc gaatatgagg tcggtgatga aattaagagc
    24661 gttattgaag acgcagaagg tagagtcatc gtagcagcag ttgcaagtaa tctcattcgt
    24721 attcaacaag tctttgatgc agcggcagaa aacggtcgtc gagtagtttt gactggtttt
    24781 gatattgaaa atattgttcg tacggccatt cgtatgaaac gcattcatat tgctgatgaa
    24841 aatatgatta tcaaacctaa agatatgaca cgttacgaag ataacgaatt attaatcctt
    24901 gaaactggac gtatgggaga gccgataaat ggtctccaaa aaatggcaat cggacgtcat
    24961 cgttatgttc aaattaaaga tggtgattta gtatttatcg taacaacacc aagtattgca
    25021 aaagaggcag ttgtagcgcg tgttgaaaat ctgatttata aagctggtgg ctcagttaaa
    25081 ttgataacac agaatttgcg cgtatcaggt catgcaaatg gtcgcgaatt acaattatta
    25141 atgaatctat tgaaacctaa ataccttttc ccaattcaag gagagtatag ggatttgtca
    25201 gcacatgcgg gcttagctca agaggtagga atgtcagcag atgatattta tattgtgaag
    25261 cgtggtgaca tcatggttct tgaaaaagat ggcttcttcc atagtggatt tgttcctgct
    25321 ggagatgtta tgattgatgg aaatgctatt ggtgacgttg gtaatattgt acttcgtgac
    25381 cgcaaggtat tatctgaaga tggaattttt attgttgtta taactgtcag caaaaaagaa
    25441 aagaaaatca tttctaaagc tcgtgttaat acgcgtggtt ttgtgtatgt gaaaaaaagc
    25501 agagatattt tgcgtgagag cgcagaatta gttaatacaa cggtagaaga ttacttaagc
    25561 aaagatacat ttgactgggg tgaacttaaa gggaaagtgc gtgatgaggt cagcaagttc
    25621 ttatttgacc aaacgaaacg tcgcccagca atccttcctg tggttatgga agttcgttga
    25681 tagtgttaag aggttaccaa attttggtag cctttcttgc tagaatggag gtttttatgg
    25741 cattttttaa tattgaatat cactctaagg tactaggaac ggaacgtcaa gtcaatgtta
    25801 tttacccaga tgcttttgag atatctgatg ataaaattga tgattgtgac attcctgttt
    25861 tgtatcttct ccatggaatg gggggaaatg agaattcttg gcaaaaacgc acaaatatag
    25921 agcgtctttt acgtcatact aatcttatcg tcgtgatgcc atcaacagac ttagcatggt
    25981 ataccaacac aaagtatggg ttggattatt ttgatgcaat agctatcgag ttaccaaaag
    26041 ttttgaaacg tttctttcct aatatgtctg ataagagaga gaaaaatttc attgctggat
    26101 tatctatggg gggatatgga gcatataaaa tagctctact gactaatcgt ttttctcatg
    26161 ctgcctcttt gtcaggtgca ctttcttttg actttgattt attattcaat aatggcaacg
    26221 ataacattaa ctattggtcg ggaatttttg gtgatttgaa taacactgat aacatagagc
    26281 gtcattcact aaggcgttac gttgagtctt ttgatatgaa aactaaattt tatgcatggt
    26341 gtggttatga agattttctt tttgaagcta atgaagtcgc aattgatgaa cttcgtcaat
    26401 taggtttaac tattgactat ttcaatgatc atggtaaaca tgaatggtat tactggaatc
    26461 agcaattaga aaaagtctta gaatggctcc ctattgatta tgtcaaagaa gagcgccttt
    26521 cgtagtatta gctaaatcgt tgaaatccaa gtaaagtctt gttatactaa atcattaagt
    26581 tttgggaaat tagacacaga aggtgagata aaaaatgaga acactattta gaatgatatt
    26641 tgctattcca aagtttatct ttagattgat ttggaatatc atttggggaa tattcaagac
    26701 agttcttgtt attgcgatta ttttatttgg cttgtattac tatgcgaatc acagtcaatc
    26761 agaatttgct aatcaactta gtgacattat tcagacagga aaaacatttt taaattttgc
    26821 tgacactaat cagctcaaaa attcctttac taatctagct acggatagtg ttcatcattc
    26881 agaaaatacc agatgggata aaaatcaggc aactatctat atagcaacta gagattctga
    26941 gttggtctct gcttacaagc aagcaatctc caattggaat gcaacaggtg cttttgtgtt
    27001 acaaacaaca gacaatccta atgctgatat tattgctaag gattactcag atgctaaaac
    27061 acaagctgcg ggtgtagctg aaaccgagaa aaatgcttta actaatcgta tttcaaaggt
    27121 aacagttaaa ttaaacacat tttaccttaa gaataaacag tttggttatg accatactag
    27181 gattgttaat acagccgaac atgaattagg acacgcttta ggtctaggac ataatgatca
    27241 acaacattcc attatgcagt caaaaggttc gcattatggc attcaagaag ttgatattca
    27301 aacgctaaaa aacttatatg cataaaaaga tgagctaaga aactcatctt ttttgtttga
    27361 acacttattt taggtgatat aagtgcgtta gcaatagatt attaaaaata aagaatttga
    27421 ctatcgctac taactaaaat gcataaagaa caaagaaaat gttttcaaaa atcacttaaa
    27481 aaatggtata atgtgtcttg agatagtgtt ttgaatagac ttatcaaaaa tattagataa
    27541 cattaactta aaaggggaaa atatggacga actaaaaaaa cttgctggag tgaccgctgc
    27601 aaaatacgtt aaaaacggta tgattgtagg tttagggaca ggttctacag catatttttt
    27661 tgttgaagaa attggacgcc gtgtcaaaga agagggattg caagtcgtag gcgttacaac
    27721 gtcaaaccgt acgactgagc aggcaagagg attaggtatt ccgttgaaat cagcagatga
    27781 tattgatgtc attgatgtca ctgtggatgg agcagatgaa gtagaccctg attttaatgg
    27841 tatcaagggt ggcggtggtg cgctgctcat ggaaaaaata gtagctactc caactaaaga
    27901 atatatctgg gttgtcgatg agagcaaact tgttgaaacg ttaggtgcat ttaaattgcc
    27961 tgttgaggtt gttagatatg gttctgagcg tctttttcgg gtttttaaat caaaaggtta
    28021 ttgtccttct ttccgtgaaa cagaaggtga ccgctttatc actgatatgg ggaactacat
    28081 tatcgaccta gatttgaaaa aaatagagga tccaaagcag ttagcgaatg aacttgacca
    28141 tacagttggt gttgttgaac atggtttatt taatagtatg gttaataaag ttatagtagc
    28201 aggcaaaaac ggtcttgaca tacttgaaaa ataattattt aaataatcgg ttttaggagc
    28261 taaccgtaac ctccttagaa agaagatata atgtcacaat ttgatcgcat tcacttagtt
    28321 gtcttagatt cagtgggtat tggagcggca ccagatgcca atgattttgt caatgcagga
    28381 gttcctgatg gtgcttcaga cacgcttggc cacatttcaa aaacagttgg acttgcggtg
    28441 ccaaacatgg ctaaaattgg acttggtaat attcctcgtc cccaagcgct taaaaccgtt
    28501 cctgcagaag aaaatccaag tggctacgcg acgaagttac aagaagtatc acttggtaaa
    28561 gatacaatga cagggcattg ggagattatg ggcttaaata ttacagagcc atttgatact
    28621 ttctggaatg gttttccaga agatattatc acaaagattg aggatttttc aggacgtaaa
    28681 gttattcgtg aagcgaataa accttattca ggaacagctg ttattgatga cttcggacca
    28741 cgccaaatgg aaactggtga gctcatcatc tatacctcag ctgatccagt cctacaaatt
    28801 gcagctcatg aggatatcat tcctctagaa gaactctacc gtatttgtga atatgctcgt
    28861 agtatcacaa tggagcgtcc agctctcctt ggtcgtatta tcgctcgtcc atatgtaggt
    28921 gaaccaggca actttacgcg tacagccaac cgtcacgact atgcagt
//
LOCUS       DATRMA010000018        28589 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.018, whole genome shotgun sequence.
ACCESSION   DATRMA010000018 DATRMA010000000
VERSION     DATRMA010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 28589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28589
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.018"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            complement(353..739)
                     /gene="rplQ"
                     /locus_tag="VBC28_000755"
     CDS             complement(353..739)
                     /gene="rplQ"
                     /locus_tag="VBC28_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEO6814756.1"
                     /translation="MAYRKLGRTSSQRKAMLRDLTTDLLINESIVTTEARAKEIRKTV
                     EKMITLGKRGDLHARRQAAAYVRNEIASENYDEASDKYTSTTALQKLFDDIAPRYAER
                     NGGYTRILKTEPRRGDAAPMAIIELV"
     gene            complement(754..1692)
                     /locus_tag="VBC28_000756"
     CDS             complement(754..1692)
                     /locus_tag="VBC28_000756"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEO6814757.1"
                     /translation="MIEFEKPIITKIDENKDYGRFVIEPLERGYGTTLGNSLRRVLLS
                     SLPGAAVTSIKIDGVLHEFDTIPGVREDVMQIILNVKGLAVKSYVEDEKIIELDVEGP
                     AEITAGDILTDSDIEIVNPDHYLFTIAEGHSLKATMTVAKNRGYVPAEGNKKDDAPVG
                     TLAVDSIYTPVKKVNYQVEPARVGSNDGFDKLTIEIMTNGTIIPEDALGLSARVLIEH
                     LNLFTDLTEVAKATEVMKETEKVNDEKVLDRTIEELDLSVRSYNCLKRAGINTVFDLT
                     EKTEPEMMKVRNLGRKSLEEVKIKLADLGLGLKNDK"
     gene            complement(1742..2125)
                     /gene="rpsK"
                     /locus_tag="VBC28_000757"
     CDS             complement(1742..2125)
                     /gene="rpsK"
                     /locus_tag="VBC28_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEO6814758.1"
                     /translation="MAKPTRKRRVKKNIESGVAHIHATFNNTIVMITDVHGNALAWSS
                     AGALGFKGSRKSTPFAAQMAAEAAAKSAQEHGLKTVEVTVKGPGSGRESAIRALAAAG
                     LEVTAIRDVTPVPHNGARPPKRRRV"
     gene            complement(2143..2508)
                     /gene="rpsM"
                     /locus_tag="VBC28_000758"
     CDS             complement(2143..2508)
                     /gene="rpsM"
                     /locus_tag="VBC28_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEO6814759.1"
                     /translation="MARIAGVDIPNDKRVVISLTYVYGIGLSTSKKILAAAGISEDIR
                     VKDLTPDQEDAIRREVDAIKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGKAVAIAGKKK"
     gene            complement(2526..2642)
                     /gene="rpmJ"
                     /locus_tag="VBC28_000759"
     CDS             complement(2526..2642)
                     /gene="rpmJ"
                     /locus_tag="VBC28_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEO6814760.1"
                     /translation="MKVRPSVKPICEYCKVIRRNGRVMVICPTNPKHKQRQG"
     gene            complement(2668..2886)
                     /gene="infA"
                     /locus_tag="VBC28_000760"
     CDS             complement(2668..2886)
                     /gene="infA"
                     /locus_tag="VBC28_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEO6814761.1"
                     /translation="MAKEDVIEIEGKVVETMPNAMFTVELENGHQILATVSGKIRKNY
                     IRILVGDRVTVEMSPYDLTRGRITYRFK"
     gene            complement(3002..3640)
                     /locus_tag="VBC28_000761"
     CDS             complement(3002..3640)
                     /locus_tag="VBC28_000761"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEO6814762.1"
                     /translation="MNLLIMGLPGAGKGTQAAKIVEEFGVAHISTGDMFRAAMANQTE
                     MGRLAKSYIDKGELVPDEVTNGIVKERLAEDDIAEKGFLLDGYPRTIEQAHALDATLE
                     ELGLRLDGVINIKVDPSCLIERLSGRIINRKTGETFHKVFNPPVDYKEEDYYQREDDK
                     PETVKRRLDVNIAQGEPILEHYRKLGLVTDIEGNQEITEVFADVEKALLELK"
     gene            complement(3735..5039)
                     /gene="secY"
                     /locus_tag="VBC28_000762"
     CDS             complement(3735..5039)
                     /gene="secY"
                     /locus_tag="VBC28_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEO6814763.1"
                     /translation="MFLKLLRDALKVKMVRNKILFTIFILLVFRIGTHITVPGINVKS
                     LEQMGELPFLNMLNLVSGNAMRNFSVFSMGVSPYITASIVVQLLQMDILPKFVEWGKQ
                     GEVGRRKLNQATRYISLFLAFVQSIGITAGFNTLSSVALVKTPNVQTYLLIGAILTTG
                     SMVVTWLGEQITDKGFGNGVSMIIFAGIISSIPSAITTIYEDFFVNVRSSAITNSYIF
                     VGILIVAVLAIVFFTTFIQQAEYKIPIQYTKLVQGAPTSSYLPLKVNPAGVIPVIFAS
                     SITTIPSTIIPFFQNGKEIPWLTKLQELLNYQTPVGMIIYAILIILFSFFYTFVQVNP
                     EKTAENLQKNSSYIPSIRPGRETEEYMSSLLKKLATIGSVFLAFISLLPIIAQQALHL
                     SSSIALGGTSLLILIATGIEGMKQLEGYLLKRRYVGFMNTTE"
     gene            complement(5060..5500)
                     /gene="rplO"
                     /locus_tag="VBC28_000763"
     CDS             complement(5060..5500)
                     /gene="rplO"
                     /locus_tag="VBC28_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004225266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEO6814764.1"
                     /translation="MKLHELKPAEGSRKVRNRVGRGTSSGNGKTSGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRMPKRGFSNINAKEYALVNLDQLNVFEDGTEVTPVVLKEAGIV
                     RAEKSGVKILGNGELTKKLSVKAAKFSKSAEAAITAKGGSIEVI"
     gene            complement(5625..5804)
                     /gene="rpmD"
                     /locus_tag="VBC28_000764"
     CDS             complement(5625..5804)
                     /gene="rpmD"
                     /locus_tag="VBC28_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEO6814765.1"
                     /translation="MAQIKITLTKSPIGRKPEQRKTVVALGLGKLNSSVVKEDNAAIR
                     GMVNAISHLVTVEEA"
     gene            complement(5819..6313)
                     /gene="rpsE"
                     /locus_tag="VBC28_000765"
     CDS             complement(5819..6313)
                     /gene="rpsE"
                     /locus_tag="VBC28_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEO6814766.1"
                     /translation="MAFKDNAVELEERVVAINRVTKVVKGGRRLRFAALVVVGDRNGR
                     VGFGTGKAQEVPEAIRKAVEAAKKNMVEVPMVGTTIPHEVRSEFGGAKVLLKPAVEGA
                     GVAAGGAVRAVIELAGVADITSKSLGSNTPINIVRATVEGLKQLKRAEEVAALRGISV
                     SDLA"
     gene            complement(6332..6688)
                     /gene="rplR"
                     /locus_tag="VBC28_000766"
     CDS             complement(6332..6688)
                     /gene="rplR"
                     /locus_tag="VBC28_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEO6814767.1"
                     /translation="MISKPDKNKIRQKRHRRVRGKLSGTADRPRLNIFRSNTGIYAQV
                     IDDVAGVTLASASTLDKEVSNGTKTEQAVVVGKLVAERAVAKGISEVVFDRGGYLYHG
                     RVKALADSARENGLKF"
     gene            complement(6789..7325)
                     /gene="rplF"
                     /locus_tag="VBC28_000767"
     CDS             complement(6789..7325)
                     /gene="rplF"
                     /locus_tag="VBC28_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEO6814768.1"
                     /translation="MSRIGNKVITLPAGVEIINKDNVVTVKGPKGQLTREFNKNIGIT
                     VEGTEVTVTRPNDSKEMKTIHGTTRANLNNMVVGVSEGFKKALEMRGVGYRAQLQGSK
                     LVLSVGKSHQDEVEAPEGVTFEVPTPTTINVIGINKESVGQTAAYVRSLRSPEPYKGK
                     GIRYVGEFVRRKEGKTGK"
     gene            complement(7435..7833)
                     /gene="rpsH"
                     /locus_tag="VBC28_000768"
     CDS             complement(7435..7833)
                     /gene="rpsH"
                     /locus_tag="VBC28_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEO6814769.1"
                     /translation="MVMTDPIADFLTRIRNANQAKHEVLEVPASNIKKGIADILKREG
                     FVKNVEVIEDDKQGIIRVFLKYGQNGERVITNLKRISKPGLRVYTKHEDMPKVLNGLG
                     IAIVSTSEGLLTDKEARQKNIGGEVLAYIW"
     gene            complement(7988..8173)
                     /locus_tag="VBC28_000769"
     CDS             complement(7988..8173)
                     /locus_tag="VBC28_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HEO6814770.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCEKCGRPHSVYRKFQLCRVCF
                     RDLAYKGQVPGVTKASW"
     gene            complement(8191..8733)
                     /gene="rplE"
                     /locus_tag="VBC28_000770"
     CDS             complement(8191..8733)
                     /gene="rplE"
                     /locus_tag="VBC28_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEO6814771.1"
                     /translation="MANRLKEKYTNEVVPALTEKFNYSSVMAVPKVEKIVLNMGVGDA
                     VSNAKNLEKAAAELALISGQKPLITKAKKSIAGFRLREGVAIGAKVTLRGERMYEFLD
                     KLVSVSLPRVRDFHGVPTKSFDGRGNYTLGVKEQLIFPEINFDDVDKVRGLDIVIVTT
                     ANTDEESRELLKGLGMPFAK"
     gene            complement(8757..9062)
                     /gene="rplX"
                     /locus_tag="VBC28_000771"
     CDS             complement(8757..9062)
                     /gene="rplX"
                     /locus_tag="VBC28_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEO6814772.1"
                     /translation="MFVKKGDKVRVIAGKDKGTEAVVLKALPKVNKVVVEGVALIKKH
                     QKPNNENPQGAIVEKEAPIHVSNVQVLDKNGVAGRVGYKVVDGKKVRYNKKSGEVLD"
     gene            complement(9142..9510)
                     /gene="rplN"
                     /locus_tag="VBC28_000772"
     CDS             complement(9142..9510)
                     /gene="rplN"
                     /locus_tag="VBC28_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEO6814773.1"
                     /translation="MIQQETRLKVADNSGAREILTIKVLGGSGRKFANIGDVIVASVK
                     QATPGGAVKKGDVVKAVIVRTKTGARRPDGSYIKFDDNAAVIIRDDKTPRGTRIFGPV
                     ARELREGGYMKIVSLAPEVL"
     gene            complement(9535..9795)
                     /gene="rpsQ"
                     /locus_tag="VBC28_000773"
     CDS             complement(9535..9795)
                     /gene="rpsQ"
                     /locus_tag="VBC28_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEO6814774.1"
                     /translation="MERNQRKTLYGRVVSDKMDKTITVVVETKRNHPVYGKRINYSKK
                     YKAHDENNVAKEGDIVRIMETRPLSATKRFRLVEVVEKAVII"
     gene            complement(9821..10027)
                     /locus_tag="VBC28_000774"
     CDS             complement(9821..10027)
                     /locus_tag="VBC28_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEO6814775.1"
                     /translation="MKLQEIKDFVKELRGLSQEELAKKENELKKELFDLRFQAAAGQL
                     EKTARLDEVKKQIARVKTVQSEMK"
     gene            complement(10037..10450)
                     /gene="rplP"
                     /locus_tag="VBC28_000775"
     CDS             complement(10037..10450)
                     /gene="rplP"
                     /locus_tag="VBC28_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003029477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEO6814776.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVSFGEYGLQATTSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKV
                     MFEIAGVSEEVAREALRLASHKLPVKCKFVKREAE"
     gene            complement(10454..11107)
                     /gene="rpsC"
                     /locus_tag="VBC28_000776"
     CDS             complement(10454..11107)
                     /gene="rpsC"
                     /locus_tag="VBC28_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEO6814777.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYADYLHEDLAIRKFINK
                     ELADASVSTIEIERAVNKVIVSLHTAKPGMVIGKGGANVDALRGQLNKLTGKQVHINI
                     IEIKQPDLDAHLVGENIARQLEQRVAFRRAQKQAIQRTMRAGAKGIKTQVSGRLNGAD
                     IARAEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEVLPARKNTKGGK"
     gene            complement(11120..11464)
                     /gene="rplV"
                     /locus_tag="VBC28_000777"
     CDS             complement(11120..11464)
                     /gene="rplV"
                     /locus_tag="VBC28_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEO6814778.1"
                     /translation="MAEITSAKAMARTVRVSPRKTRLVLDLIRGKNVADAIAILKFTP
                     NKAARVIEKTLNSAIANAENNFGLEKANLVVSETFANEGPTMKRFRPRAKGSASPINK
                     RTTHVTVVVSEK"
     gene            complement(11480..11758)
                     /gene="rpsS"
                     /locus_tag="VBC28_000778"
     CDS             complement(11480..11758)
                     /gene="rpsS"
                     /locus_tag="VBC28_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEO6814779.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQANDEKKKVIKTWSRRSTIFPSF
                     IGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYKGHAADDKKTRR"
     gene            complement(11857..12690)
                     /gene="rplB"
                     /locus_tag="VBC28_000779"
     CDS             complement(11857..12690)
                     /gene="rplB"
                     /locus_tag="VBC28_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019790357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEO6814780.1"
                     /translation="MGIKVYKPTTNGRRNMTSLDFAEITTNTPEKSLLVSLKNKAGRN
                     NNGRITVRHQGGGHKRHYRLIDFKRNKDGVEAVVKTIEYDPNRTANIALVHYTDGVKA
                     YILAPKGLEVGQRIISGPEADIKVGNALPLANIPVGTVIHNIELQPGKGAELIRAAGA
                     SAQVLGQEGKYVLVRLQSGEVRMILGTCRATIGTVGNEQQSLVNIGKAGRNRWKGVRP
                     TVRGSVMNPNDHPHGGGEGKAPVGRKAPSTPWGKPALGLKTRNKKAKSDKLIVRRRNQ
                     K"
     gene            complement(12708..13004)
                     /locus_tag="VBC28_000780"
     CDS             complement(12708..13004)
                     /locus_tag="VBC28_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEO6814781.1"
                     /translation="MNLYDVIKKPVITEKSMVALEAGKYTFEVDTRAHKLLIKQAVEA
                     VFDGVKVASVNTVTVKPKAKRVGRYTGFTSKTKKAIITLTADSKAIELFAAEAE"
     gene            complement(13004..13627)
                     /gene="rplD"
                     /locus_tag="VBC28_000781"
     CDS             complement(13004..13627)
                     /gene="rplD"
                     /locus_tag="VBC28_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEO6814782.1"
                     /translation="MANVKLFDQTGKEVSSVELNEAIFGIEPNESVVFDVVISQRASL
                     RQGTHAVKNRSAVSGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYGYKLP
                     QKVRRLALKSVYSAKVAEDKFVAVENLSFAAPKTAEFASVLSALSIDSKVLVILEEGN
                     EFAALSARNLPNVTVATATTASVLDIVNADKLLVTKEAISTIEGVLA"
     gene            complement(13651..14277)
                     /gene="rplC"
                     /locus_tag="VBC28_000782"
     CDS             complement(13651..14277)
                     /gene="rplC"
                     /locus_tag="VBC28_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEO6814783.1"
                     /translation="MTKGILGKKVGMTQIFTESGEFIPVTVIEATPNVVLQVKTVETD
                     GYEAVQVGFDDKREVLSNKPAKGHVAKANTAPKRFIREFKNIEGLEVGAELSVEQFEA
                     GDVVDVTGTSKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRLAG
                     RMGGNRVTVQNLEIVQVIPEKNVVLIKGNVPGAKKSLITIKSAVKAAK"
     gene            complement(14382..14690)
                     /gene="rpsJ"
                     /locus_tag="VBC28_000783"
     CDS             complement(14382..14690)
                     /gene="rpsJ"
                     /locus_tag="VBC28_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEO6814784.1"
                     /translation="MANKKIRIRLKAYEHRTLDTAAEKIVETATRTGATVAGPVPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLVDIINPTQKTVDALMKLDLPSGVNVEIKL
                     "
     gene            complement(14896..16176)
                     /locus_tag="VBC28_000784"
     CDS             complement(14896..16176)
                     /locus_tag="VBC28_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001270709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEO6814785.1"
                     /translation="MRYSKEIIQLAIPAMIENILQMLMGVVDNYLVAQLGVVAVSGVS
                     VANNIITIYQAIFIALGASIASLLAKSLAGSKKDDAISVCSQAIFLTLLIGAVLGIIS
                     IVFGQTFFKLLGTTKSVAQVGGLYLAIVGGGVVTLGMLTTLGSFLRVQGQPRLPMYVS
                     IFVNFLNAVLSGFAIFEWRYGLVGVAVSTLIARLIGICILAKYLPIKKIIKRMTWKIS
                     AQIWNLALPSAGERLMMRAGDVVIVAIVVQLGTNVVAGNAIGETLTQFNYMPGLGIAT
                     ATIILTAKYVGQKNRESIEETIQSSYYIGLVLMILISSFMLLAGKPLTQLFTNNPSAI
                     KGSLIVILLSFVGVPATIGTLVYTAAWQGLGNAKLPFYTTTIGMWLIRVVLGYLLGIV
                     FELGLLGVWMATIADNIFRWLFLKVHYHRYIQKM"
     gene            complement(16281..17771)
                     /locus_tag="VBC28_000785"
     CDS             complement(16281..17771)
                     /locus_tag="VBC28_000785"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEO6814786.1"
                     /translation="MAIVYQSTRDASNKVTASQAILKGLADDGGLFTPITFPKVDLDF
                     TKLKDASYQEVAKLVLSAFFDDFTEQELDYCISQAYDTKFDTTEIAPIVKIGDRYHLE
                     LFHGPTIAFKDMALSILPYLLTTAAKKQGVDNKIVILTATSGDTGKAAMAGFADVPGT
                     EIIVFYPKNGVSYIQELQMITQAGQNTHVVAIEGNFDDAQTSVKEMFNNSLLRLKLSQ
                     QHMQLSSANSMNIGRLVPQIVYYIYAYAQLVKSKEISIGQPINFSVPTGNFGNILAAY
                     YASQIGLPVTKLICASNDNNVLTDFFKTQTYDKNREFKVTSSPSMDILVSSNLERLIF
                     HLLGDDAETTKKLMEDLVTTGEYALEARQANILESFVAGFATEQFVELDIKHLFDQYQ
                     YIEDPHTAVASAVYQAYQTETKDQTPAVIVSTASPYKFPCVVTKAITNKEEIQDFAAI
                     SILNDLSGVSLPKAVTDLQKAEVIHRTVVPTSNMRETVEECLGLKQ"
     gene            complement(17891..18907)
                     /gene="adhP"
                     /locus_tag="VBC28_000786"
     CDS             complement(17891..18907)
                     /gene="adhP"
                     /locus_tag="VBC28_000786"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HEO6814787.1"
                     /translation="MKAVVVNQASTGVEVVEHDLPKVGHGEALVKVEYCGVCHTDLHV
                     AHGDFGQVPGRILGHEGIGIVEEIGEGVTSLRVGDRVSIAWFFEGCGHCEYCTTGRET
                     LCRSVKNAGYSVDGGMSEYAIVTADYAVKVPEGLDPAQASSITCAGVTTYKAIKEAGA
                     APGQWIAVYGAGGLGNLAVQYAKKVFNAHVVAVDINADKLQLAKEVGADLTVNGKEIK
                     DVAAYIQEKTGGCHGVVVTAVSKVAFNQAIDSVRAGGTVVAVGLPSEYMELSIVKTVL
                     DGIRVVGSLVGTRKDLEEAFAFGAEGLVVPVVEKVPVDTAPQVFDEMERGLIQGRKVL
                     DFTK"
     gene            complement(19086..21728)
                     /gene="adhE"
                     /locus_tag="VBC28_000787"
                     /gene_synonym="adhC"
     CDS             complement(19086..21728)
                     /gene="adhE"
                     /locus_tag="VBC28_000787"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002917892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HEO6814788.1"
                     /translation="MTEKTKAVETTDVALAIDTLVQNGLKALDEMRQLNQEQVDYIVA
                     KASVAALDAHGELALHAVEETGRGVFEDKATKNLFACEHVVNNMRHTKTVGVIEEDDV
                     TGLTLIAEPVGVVCGITPTTNPTSTAIFKSLISLKTRNPIIFAFHPSAQESSAHAARI
                     VRDAAIAAGAPENCVQWIEQPSIDATNALMNHDGIATILATGGNAMVKAAYSCGKPAL
                     GVGAGNVPAYVEKSANIRQAAHDIVMSKSFDNGMVCASEQAVIIDKEIYKEFVEEFKS
                     YHTYFVNKKEKALLEEFCFGAKANSKNCAGAKLNPNIVGKSAVWIAEQAGFTVPEGTN
                     ILAAECTEVSEKEPLTREKLSPVIAVLKAESTEDGVEKARQMVEFNGLGHSAAIHTKD
                     ADLAREFGTRIRAIRVIWNSPSTFGGIGDVYNAFLPSLTLGCGSYGRNSVGDNVSAIN
                     LLNIKKVGRRRNNMQWFKVPSKTYFERDSIQYLQKCRDVERVMIVTDHAMVELGFLDR
                     IIEQLDLRRNKVVYQIFAEVEPDPDITTVMKGTDLMRTFKPDTIIALGGGSPMDAAKV
                     MWLFYEQPEVDFHDLVQKFMDIRKRAFKFPELGKKTKFVAIPTTSGTGSEVTPFAVIS
                     DKANNRKYPIADYSLTPTVAIVDPALVMTVPGFIAADTGMDVLTHATEAYVSQMANDY
                     TDGLALQAIKIVFDYLERSVKDADFEAREKMHNASTMAGMAFANAFLGISHSMAHKIG
                     AQFHTVHGRTNAILLPYVIRYNGTRPAKTATWPKYNYYRADEKYQDIAKLLGLPAATP
                     EEAVESYAKAVYDLGTRLGIKMNFRDQGIDEKEWKEKSRELAFLAYEDQCSPANPRLP
                     MVDHMQEIIEDAYYGYEERPGRRK"
     gene            complement(21998..23776)
                     /locus_tag="VBC28_000788"
     CDS             complement(21998..23776)
                     /locus_tag="VBC28_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEO6814789.1"
                     /translation="MRIKWFSLVRITGLLLVLLYHFFKNSFPGGFVGVDIFFTFSGFL
                     ITALLIDEFSKTKKIDFVSFCRRRFYRIFPPLVLMVLVTIPFVFLVKSDFRASIGSQI
                     MTALGFTSNFYEILTGGNYESQFIPHLFVHTWSLSIEVHFYVLWGLTVWLLSKRSKDQ
                     KQLRGTLFLISMGIFGVSFLTMFVRAFFVDNFSTIYFSTLSHIFPFFLGAMVATISGI
                     REITGRFKKNIKNLTLKHNLIMMGSAFAGLMILTFALDFDNRLTYLFGFVLSSIFASV
                     MIYNARILHEHTPDISEPFVITYLADISYGMYLFHWPFYIIFSRLSPNWIAVILTVVL
                     SAVFSTLSFYIIEPFILGRKPKFLDYEFDLLPYKKWLFSIGGVLTLITVVTMLTAPSI
                     GSFETELLQNSLQQARTNTNRTHTLAAGDAGALSDVTVIGDSVALRSSAAFNKLLPEV
                     QLDAAVSRNFSKSFDIFENRIQNKALSKIVVLAVGVNSLDNYKTDLSQFIKSLPKGHR
                     LIIVTPYNAKNMSQVTTVRDYELSLMKKYNYITVADWYKVATEHPEIWGNTDGVHYSD
                     SDTTGADLYVSNVKKAIQKSAQRAAK"
     gene            complement(23773..24153)
                     /locus_tag="VBC28_000789"
     CDS             complement(23773..24153)
                     /locus_tag="VBC28_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6814790.1"
                     /translation="MKNFKITRTHLEILSLIIIVVFGLSVFTLTTSSQGVFSYDGGKI
                     KYVGSIVNHHMTGKGKLTYENGDYYKGDFVNGVFEGKGTFVSVHGWSYTGDFKKGQPD
                     GQGRLNAKNKKVYKGTFKQGIYQK"
     gene            complement(24160..24597)
                     /locus_tag="VBC28_000790"
     CDS             complement(24160..24597)
                     /locus_tag="VBC28_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HEO6814791.1"
                     /translation="MKKVCFVCLGNICRSPMAEFVMANLDKEDSLLVESRATSTWEHG
                     NPIHRGTQAILQRYQIPYINDKGSQPISKDDLAYFDYIIGMDEQNVKDLKALSQGTYD
                     DKISLFCDGGVPDPWYTGDFEETYRLVNDGCKQWLTLINNSRD"
     gene            complement(24749..25747)
                     /gene="ruvB"
                     /locus_tag="VBC28_000791"
     CDS             complement(24749..25747)
                     /gene="ruvB"
                     /locus_tag="VBC28_000791"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001902090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEO6814792.1"
                     /translation="MTRFLDSDAMGDEELVERTLRPQYLREYIGQDKVKDQLKIFIEA
                     AKLRDESLDHVLLFGPPGLGKTTMAFVIANELGVNLKQTSGPAIEKSGDLVAILNDLE
                     PGDVLFIDEIHRMPMAVEEVLYSAMEDFYIDIMIGAGETSRSVHLDLPPFTLIGATTR
                     AGMLSNPLRARFGITGHMEYYEENDLTEIIERTADIFEMKITYEAASELARRSRGTPR
                     IANRLLKRVRDYAQIMGDGLIDDNITDKALTMLDVDHEGLDYVDQKILRTMIEMYNGG
                     PVGLGTLSVNIAEERDTVEDMYEPYLIQKGFIMRTRTGRVATVKAYEHLGYQRFDK"
     gene            complement(26044..26955)
                     /locus_tag="VBC28_000792"
     CDS             complement(26044..26955)
                     /locus_tag="VBC28_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO6814793.1"
                     /translation="MLKHFGSKVRNLRVTRNITREDFCGDETELSVRQLARIESGQSI
                     PNLTKAHYIAKQLNVKLDILTGGESLELPKRYKELKYLILRIPTYADAERLKLRECQF
                     DHIFEEFYDNLPEDECLAIDSLQAKFEVYQTGDINFGVEVLCECFDKVKYKEKYTLND
                     LIIIDLFLTCAVVSKFNNRAFTKEVFQTICKTLISQNHKLTAEDLFWFNHVLLNCVFV
                     GLCLNSEECLAEMLEVSRQTMVSTHDFHKMPLYFMYQWKYFITIDNDIKSAENAYQQS
                     IMFSKMIDDKHLIKKLELEWQEDITGH"
     gene            complement(27107..28405)
                     /gene="purB"
                     /locus_tag="VBC28_000793"
     CDS             complement(27107..28405)
                     /gene="purB"
                     /locus_tag="VBC28_000793"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEO6814794.1"
                     /translation="MIERYSRPEMAAIWTEENKYRAWLEVEILADEAWAELGEIPKED
                     VAKIREKADFDIDRILEIEQDTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLYKQANDIIRRDLENFTNIVADKAKEHKFTIMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAAGVEAGKISGAVGNFANIPPFVEQYVCDKLGIRPQEISTQVLPRDL
                     HAEYFAVLASIATSIERMATEIRGLQKSEQREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MTGLARVIRGHMVTAYENVALWHERDISHSSAERIITPDTTILIDYMLNRFGNIVKNL
                     TVFPENMMRNMESTFGLIYSQRVMLKLIEKGMTREEAYDLVQPKTAYSWDNQVDFKPL
                     LEEDTKVTSCLTQEEIDELFNPIYYTKRVDDIFERLGLEK"
     gene            complement(28430..>28589)
                     /locus_tag="VBC28_000794"
     CDS             complement(28430..>28589)
                     /locus_tag="VBC28_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6814795.1"
                     /translation="FIPKLYEEYIYILISLGEVEKAKEILKEVSDYLTEESLNYFEKE
                     YDKAKGSN"
BASE COUNT         8613 a         5929 c         4825 g         9222 t
ORIGIN      
        1 attatactaa atatttctac ttcctgtcaa tactattttt gtattttata aatttagtat
       61 agacataact attcctctat attcaattaa gagaaattat ataaccacta ttgagaaatg
      121 tagtctagcg attaaattct ttgctcatcg aaaatatcca ataaatataa taatgcataa
      181 aacgcctgct tacgaaatat aaacaaaatt gtttgcattt cgtaaacaag cgttacctat
      241 ttaacaatat atgatgagtg ttcccgctga gaataattct cagcggtaga ccagagctag
      301 actaagaatc gattgattcc atcatcataa cactcaacaa aattgataaa aattatacta
      361 attcaataat tgccattggg gcagcatcgc cacggcgtgg ttctgtttta agaatacgag
      421 tgtatcctcc gttacgttca gcataacgag gtgcgatgtc gtcaaaaagt ttttgaagag
      481 ctgttgttga tgtatattta tcagaagctt catcatagtt ttctgatgca atttcattac
      541 gtacataagc agcagcttga cgacgagcat gtaaatcacc acgtttacct agagtaatca
      601 ttttttcaac tgttttacgg atttctttag cacgtgcttc agtagttaca attgattcgt
      661 tgataagaag atcggttgtc aaatcacgaa gcattgcctt acgttgtgag ctagtgcgtc
      721 ctagtttacg gtaagccatt atgtcctcct attttattta tcgtttttta atccaagacc
      781 tagatcggca agtttgattt taacttcttc aagactctta cgtcctaagt ttcggacttt
      841 catcatttca ggctcagttt tttctgttaa atcaaatact gtattaattc cagcacgttt
      901 taaacagtta tatgagcgca ctgacaaatc aagttcctca attgtccggt caagcacttt
      961 ctcatcgttc actttctctg tttccttcat tacttcagtt gctttagcaa cctctgttaa
     1021 atcagtaaac aagtttaagt gttcaattaa gacgcgagct gaaagaccaa gagcatcctc
     1081 aggaatgatt gtgccatttg tcatgatttc aatagttaat ttatcaaagc catcgttact
     1141 acctacacga gcaggttcaa cttgataatt aacttttttc actggtgtat agattgaatc
     1201 tacagccaat gttcccactg gtgcatcatc ttttttattt ccttctgctg gaacataacc
     1261 tcggttttta gcaactgtca ttgttgcttt caaactgtga ccttcagcaa ttgtgaaaag
     1321 ataatggtca gggtttacaa tttcgatatc actatctgtt aaaatatctc cggcagtaat
     1381 ttctgcagga ccttcaacgt ctagttcgat aatcttttcg tcttcgacgt aagatttcac
     1441 agcaagtcct tttacattaa ggatgatttg catcacatct tcacgtacac ctgggatagt
     1501 atcaaattcg tgtaatactc catcaatttt gattgatgtg actgctgcac ctggaagtga
     1561 agacagaagc acacgacgaa gagagtttcc tagagttgta ccgtagccac gttctagtgg
     1621 ttcgatgaca aatctgccgt aatctttatt ttcatcaatt tttgttatta ttggtttttc
     1681 aaactcaatc atttagttac tccctcttaa acgaaaagtt gtgtactata aaatgctatg
     1741 attatacacg acgacgtttt ggaggacgag caccattatg tggtacagga gtcacatcac
     1801 gaattgcagt tacttcaaga ccagctgcag caagggcacg gatagcagat tcacgacctg
     1861 aaccaggacc ttttacagta acttcaacag ttttaagacc gtgttcttgt gcagatttag
     1921 cagcagcttc agcagccatt tgagcagcaa atggagtaga tttacgagaa cctttaaatc
     1981 caagagcacc agctgatgac catgcaagag cgttaccatg cacatctgta atcataacaa
     2041 tagtgttatt gaatgtagcg tgaatatgag caacaccaga ttcgatgttc tttttcacac
     2101 gacgtttgcg tgttggttta gccaattgtt ttacctccta ttttattttt tcttacctgc
     2161 aatcgcaaca gctttacctt tacgagtgcg agcgttattt ttagtatttt gtccacggac
     2221 aggaagtcca cgacggtgac ggattccacg gtatgaaccg atttccatca aacgtttaat
     2281 attcaagttt acctcacgac gtaggtcacc ttcaactttg attgcatcaa cttcacgacg
     2341 gatagcgtcc tcttgatctg gtgtcaaatc tttaacgcgg atatcttcag aaatacctgc
     2401 agctgctaag atttttttag atgtagaaag accaattccg tatacataag taagtgaaat
     2461 tactacacgt ttgtcatttg gaatatcaac tccagcaata cgagccattt tatctccttt
     2521 ctatattatc cttgacgttg tttgtgtttt gggtttgtcg gacaaattac cataacacga
     2581 ccgttacgac gaataacttt acagtattcg caaattggtt taaccgatgg tcttaccttc
     2641 atgttttaat ccctccaatt atttcgacta tttaaagcgg tatgtgatgc gtccacgtgt
     2701 taaatcgtat gggctcattt caacagtaac acgatcacct accaaaatac ggatgtagtt
     2761 tttacggatt tttcctgata cagttgccaa aatttggtgt ccattttcca actcaacagt
     2821 aaacattgcg ttaggcatag tttcaacaac tttaccttca atttcaatca catcttcttt
     2881 tgccacgcaa aagtaccccc taaatttcga tttgatgtcc tctataaaga ggtaacaatt
     2941 ataagtcaga ctaacttagt atatcattac taatctactt atgcaagtag attagcataa
     3001 tttatttgag ttctagcaac gctttttcaa catctgcaaa aacttctgtt atttcttgat
     3061 taccttcaat atctgtaaca agaccaagct tacgatagtg ttcaagaata ggttctcctt
     3121 gagcaatatt aacgtccaag cgacgtttga cagtttcagg cttatcatct tcacgttgat
     3181 agtaatcttc ttctttataa tctactggtg ggttgaacac tttgtggaaa gtttcaccag
     3241 ttttacgatt gataatacga ccactcaaac gctctataag acatgatgga tccactttaa
     3301 tattaataac accatctaag cgtagtccta gttcttcaag cgtagcatct aaggcgtgtg
     3361 cttgttcaat agtacgtgga tatccatcaa gtaaaaaacc tttttctgcg atatcatcct
     3421 cagctaagcg ctcttttaca atcccgtttg ttacttcatc aggaaccaat tcacctttat
     3481 caatataact tttagctaaa cgtcccattt cggtttgatt agccattgcg gcgcggaaca
     3541 tatcccctgt tgagatgtga gcaacaccaa attcttcaac gatcttagct gcttgagtac
     3601 ctttaccagc accaggcaaa cccataatta aaagattcat tatagtctcc ttattttgtt
     3661 tttaaataaa tccaacacag catgtcggac ttattaaaaa acaaaataga aggttgacag
     3721 agtcaaccta tattctattc tgttgtattc atgaatccaa catagcgtct cttcaaaaga
     3781 tagccctcga gttgtttcat accttcaata cccgttgcaa tcaaaatgag taagcttgtc
     3841 ccacctaacg caatacttga tgacaaatgc aacgcttgtt gagcaataat tggtaacaag
     3901 gaaataaatg ctagaaatac tgaaccaatt gttgctaatt ttttcaatag cgatgacata
     3961 tattcttctg tttctcgtcc tggtcgaata cttggaatgt aggacgaatt tttttgaaga
     4021 ttctctgctg tcttttcagg gtttacttgt acaaaagtat agaagaaaga gaacagaata
     4081 atcaagatag catagataat cataccgact ggggtttgat aattaagtaa ttcttggagt
     4141 ttagtaagcc aaggaatttc tttgccattt tgaaagaaag gaataatcgt acttggaatt
     4201 gttgtaatcg agctagcaaa gataacggga ataacaccag ctggatttac ttttagagga
     4261 agatatgaac ttgtaggagc tccttgaaca agttttgtat attggattgg aattttgtat
     4321 tcagcttgtt gaataaatgt tgtaaaaaat acaattgcta atactgctac gataaggatc
     4381 ccgacaaaga tgtaagaatt tgtaattgct gaagagcgta catttacaaa gaaatcttcg
     4441 tagatagttg taattgcact tggaatggaa gaaatgatac ccgcaaagat aatcatagac
     4501 actccatttc cgaatccctt atcagtaatt tgttcaccta gccaagtaac caccatacta
     4561 cctgttgtaa ggattgcacc aataagcaaa tatgtttgta cattcggagt tttaactaaa
     4621 gcaacacttg atagagtgtt aaaaccagca gtaataccaa ttgattgtac gaatgcaagg
     4681 aacaaagaaa tataacgtgt agcttggtta agcttacgac gacctacctc tccttgtttt
     4741 ccccattcaa caaacttcgg aagaatatcc atttgaagta actgaacaac aatagacgca
     4801 gtaatgtaag ggctaactcc catagagaat accgaaaagt ttctcattgc attaccacta
     4861 actaagttaa gcatatttag aaatggaagt tcacccattt gttctaaact cttaacattg
     4921 atgcccggta ctgtgatatg cgttccgata cgaaatacaa gaaggataaa gatagtaaat
     4981 aaaattttat ttcttaccat ctttactttg agtgcatctc taagtaattt taaaaacata
     5041 atgagttacc tctcattaat tagatgactt cgattgaacc acctttagca gtgatagctg
     5101 cttcagcaga tttagaaaat ttagctgctt taacagacaa tttcttagtt aattcaccat
     5161 taccaagaat tttaacgcct gatttttcag cacgcacgat tccagcttct ttaagaacaa
     5221 ctggagttac ttcagtacca tcttcaaaga catttaattg gtcaaggttc acaagagcat
     5281 attcttttgc attgatgttt gagaacccac gttttggcat acgacggaac aatggagttt
     5341 gtccaccttc aaaaccaagg cgtactccac caccactacg cgctttttgt cctttttgac
     5401 cacgtccaga agttttacca ttacctgaag atgtaccgcg acccacacga ttgcggactt
     5461 tacgtgatcc ttctgcaggt tttaattcat gaagtttcat tattttttct ccttatttgt
     5521 aaaatgctaa cgcctttacg agggaaaagg tactccccgc aaagactcgc ctatacgatt
     5581 tgtttaaatc gcaccaataa caatcagtgc gatttagcat ttaattaagc ttcttcaaca
     5641 gttactaagt gagagatagc gttaaccata ccacggatgg cagcattatc ttccttaaca
     5701 actgaagagt ttaatttacc aagtccaagg gcaacaactg ttttacgttg ttctggtttg
     5761 cgaccgattg gagacttagt caaagtaatt ttaatttgag ccattataat cccctttctt
     5821 atgctaagtc agaaacagaa atgccacgta aagcagcaac ttcttctgca cgttttaatt
     5881 gtttcaagcc ctcaacagtt gcgcgaacaa tgttgattgg agtgtttgaa ccaagtgatt
     5941 tagatgtaat atctgcaaca cctgctaatt cgatgacggc acgaacagca cccccagcgg
     6001 caaccccagc accctcaaca gctggtttca acaatacttt cgctccacca aattctgaac
     6061 gaacttcgtg aggaattgtt gtaccgacca taggaacttc aaccatgttt ttcttagcag
     6121 cttcaacagc tttgcgaata gcttctggta cttcttgagc tttacctgta ccaaaaccaa
     6181 cacgaccatt acggtcacca acaactacaa gagctgcaaa acgtagacga cgtccacctt
     6241 taacaacttt tgtaacgcgg ttaatcgcaa caacgcgctc ttcaagttct actgcattat
     6301 ctttaaatgc cattattaag tgtcctccct attagaattt caatccgttt tcacgagctg
     6361 agtcagccaa agcttttaca cgtccgtgat agagatatcc accgcggtca aacaccactt
     6421 cagaaatacc tttagctact gcgcgttcag caacaagttt accgacaaca acggcttgtt
     6481 ctgtttttgt tccgttagaa acttctttat caagagttga tgcacttgcg agcgttacac
     6541 ccgctacgtc atcaattact tgagcgtaga tgcctgtatt agaacggaaa atgttcaaac
     6601 gtgggcgatc agcagttcca gagagtttac cgcgaacacg acggtggcgt ttttggcgga
     6661 ttttattttt atctggtttc gaaatcacaa ttttcacctc ttaaatatat tgttatgtca
     6721 aaatgacaat attaaaatga aaagatagag aatttgcaga aaagattcta caaaatctat
     6781 caatcctatt atttacctgt tttaccttct ttacggcgta cgaattcacc aacgtaacga
     6841 atacctttac ctttatatgg ttctggtgaa cgtaagctac gaacgtaagc agcagtttgt
     6901 ccaacgcttt ctttgttgat accgattacg ttgattgttg ttggagttgg cacttcaaaa
     6961 gtaacacctt ctggagcttc aacttcgtct tggtgagact taccaacaga aagaacaagt
     7021 tttgatcctt gaagttgtgc acggtaacca acaccgcgca tttcaagagc ttttttgaaa
     7081 ccttcagaaa caccaacaac catgttgttc aagttcgcac gagttgtacc atgaattgtt
     7141 ttcatttctt ttgagtcgtt aggacgagta actgttactt cagtcccttc aacagtgatt
     7201 ccaatatttt tattaaactc acgagtgagt tggcctttag gtccttttac agtaacaaca
     7261 ttatccttgt taatgatttc aacacctgca ggcaatgtaa ttactttatt accaatacgt
     7321 gacatgttta ttttctcctg ttaaattgtc aagctgcttg agcaactagt tttcacgggg
     7381 actcatgaga acacaggatt atactgtgac tcaatgagca ttttaggtaa aattttacca
     7441 aatatatgca agtacttctc caccaatatt tttttggcga gcttctttgt cagtcaaaag
     7501 accttcagaa gttgatacaa ttgcaatacc aagtccgtta agaactttag gcatatcttc
     7561 atgtttagta taaacacgaa gacctggttt tgaaatacgt ttcaagttag tgataacacg
     7621 ttcaccattt tgtccatatt taaggaacac acggatgatg ccttgtttgt catcttcaat
     7681 aacttcaacg ttttttacaa aaccttcacg tttaaggata tcagcaatcc cttttttaat
     7741 gtttgatgca ggtacttcta acacttcatg ttttgcttgg ttagcattac gaatacgagt
     7801 taaaaagtct gcaattgggt cagtcataac cattttttaa ttctcctctt attagcagtt
     7861 tgaattgaat cacttgctaa ttaatgagtc cttacggacg ttgtaagact aggtcttagt
     7921 atatttgcgt ttaaaaatca gtgccattat caacgacacc ttttaaacac aaagcatcat
     7981 taatttatta ccaagatgct ttagttacgc ctggtacttg acctttataa gccaagtcac
     8041 ggaagcaaac acggcaaagt tggaatttgc ggtaaactga gtgtggacgt ccgcattttt
     8101 cacaacgagt gtaagcttgc gtagagaact tcgctgggcg tttgttctta gcaatcatag
     8161 attttttagc caatttattt acctcctaga ttactttgca aaaggcattc caaggccttt
     8221 tagcaattca cgtgactctt catcagtgtt agcagtagta acgataacga tatcaagacc
     8281 acgtacttta tcaacgtcat caaagttgat ttctgggaag atcaattgtt ctttaactcc
     8341 aagagtgtag tttccacgtc catcaaatga tttcgttgga acaccatgga agtcacgtac
     8401 acgtggaagt gaaacagaaa ctaatttatc caagaattcg tacatacgtt caccgcgcag
     8461 ggtaactttt gcaccgatcg ctacaccttc acgaagacgg aagccagcga ttgatttctt
     8521 agctttagta ataagtggtt tttgacctga aataagtgct aactcagcag cagctttctc
     8581 aagattttta gcgtttgata cagcatcacc aacacccatg ttaagaacga ttttctcaac
     8641 ttttggcaca gccataacag atgaatagtt aaatttttct gtcaacgcag gaacaacttc
     8701 attagtatat ttttctttta agcgatttgc cattatgctt ctcctttcct tcgtgattaa
     8761 tcaagcactt cgcctgattt tttgttgtaa cgaacttttt tgccgtcaac aactttgtaa
     8821 ccaacacgtc ctgctacacc atttttatca agaacttgaa cgtttgacac atggattgga
     8881 gcttcttttt caacgatagc gccttgaggg ttttcgttat ttggtttttg atgtttcttg
     8941 atgagggcta cgccttcaac aacaactttg tttacttttg gaagagcttt aagaactaca
     9001 gcttcagtgc ctttgtcctt accagcaata acgcgaactt tgtcgccttt ttttacaaac
     9061 atttgagttt ttctcctatt tttcttacgc ctcgaaaggc accctaggaa atcctagggg
     9121 actacgtttg ttaatctgag attaaagtac ttctggtgcc aatgaaacga tcttcatgta
     9181 gccaccttca cgcaattcac gtgcaactgg gccaaagata cgagttccgc gaggagtttt
     9241 atcgtcacgg atgattacag cagcattgtc gtcaaatttg atgtatgaac cgtctgggcg
     9301 gcgtgcacca gtttttgtac gaacgataac agctttaacc acatcacctt ttttaactgc
     9361 tccaccagga gtagcttgtt ttacagaagc aacgattacg tcaccgatgt tagcgaattt
     9421 acgtcctgaa ccaccaagta ctttgatagt caagatctca cgagcaccgc tattatcagc
     9481 aactttcaag cgagtttctt gttgaatcat ttcaattttc tccttttagt ttgattagat
     9541 aataacagct ttttccacta cttccacaag acggaaacgt tttgtagctg aaagtgggcg
     9601 agtttccatg atacgaacga tatcgccttc tttagcaacg ttgttttcat catgtgcttt
     9661 atatttttta gaatagttga tacgtttacc atagactggg tggttacgtt tagtttcaac
     9721 tacaactgtg attgttttgt ccatcttgtc agacactacg cgtccgtaaa gagttttacg
     9781 ttgattacgt tccatttaga atttctcctt tcccaatcta ttatttcatt tctgattgca
     9841 cagttttaac acgtgcaatt tgtttcttaa cttcgtcaag gcgagcagtc ttttcaagtt
     9901 gacctgctgc agcttggaaa cgaagatcaa acaattcttt tttgagttcg ttttctttct
     9961 tagcaagttc ttcttgagac aatccacgaa gctctttaac aaaatcttta atttcttgaa
    10021 gtttcatgtc ttctccttat tctgcttcac gttttacgaa tttgcattta actggtaatt
    10081 tgtggctagc aagacgtaat gcttcacgtg caacttcttc agaaacacca gcaatttcaa
    10141 acatcacttt accacgttta actggtgcta cccaaccttc aggtgcccct ttaccagaac
    10201 ccatacgtac accgatagct tttgcagtgt atgatttgtg tgggaagatt ttaatccaaa
    10261 ctttaccacc acgtttcatg taacgagtca tcgcaatacg ggcagcttcg atttgacggt
    10321 ttgtaatcca gtggctagtt gtagcttgaa gtccgtattc accgaatgaa acttctttac
    10381 cacctttagc ctcaccgcgc atttttccac ggaattcgcg acggtgttta acacgtttag
    10441 gtactaacat ttgttatttg cctcctttag tgtttttacg agctggaaga acttctccac
    10501 ggtaaatcca aactttaacg ccaagtttac cataagttgt gtcagcttct tcccaagcat
    10561 agtcgatatc agcacgaagc gtgtgaagcg gaacagttcc ttctgaataa ccttcagcac
    10621 gagcgatatc agcaccgttc aaacgaccag aaacttgagt tttaatacct ttagctcctg
    10681 cacgcattgt acgttggatt gcttgttttt gagcacgacg gaaagcaaca cgttgctcta
    10741 attgacgagc aatattttca ccaacaaggt gagcatctaa atcaggttgt ttgatttcaa
    10801 tgatgttgat gtgtacttgt tttccagtta atttgttaag ttgaccacga agagcgtcaa
    10861 cgtttgctcc acctttaccg ataaccatac ctggtttagc agtgtgaagt gaaacaataa
    10921 ctttgtttac tgcacgttca atttcaatag ttgaaactga tgcgtcagct aattctttat
    10981 taatgaattt acggattgca agatcttcat gaaggtaatc cgcgtattct ttttcagcat
    11041 accatttcgc atcccagtca cggatgattc cgacacgcat accaattgga tgtacttttt
    11101 gacccacgat tttacctcct tatttttctg atacaactac agttacgtga gttgtacgtt
    11161 tgttgattgg tgaagctgaa cctttcgcac gtggacggaa acgtttcatt gttggtcctt
    11221 cgttagcgaa tgtttcagat actaccaagt ttgctttttc caaaccaaag ttgttttctg
    11281 catttgcaat tgctgagtta agagttttct caataacacg agctgctttg tttggagtga
    11341 attttaagat tgcgattgcg tcagcaacat tcttaccacg gataagatca agtactaaac
    11401 gtgttttacg aggtgaaaca cgcactgtac gagccattgc tttagctgaa gtaatttctg
    11461 ccattgtgtc ctcctcctat taacgacgtg ttttcttgtc gtcagctgcg tgacctttgt
    11521 aagtacgtgt tggtgcaaat tcaccaagtt tgtgacctac catgtcctct tggatataaa
    11581 caggaacatg tttacgtcca tcataaactg cgattgtgta tccgatgaaa cttgggaaaa
    11641 tcgttgaacg acgtgaccaa gttttgatta cttttttctt ttcgtcattt gcttgagctt
    11701 caactttttt cattaaatgc tcgtcgacga aaggtccttt tttaagacta cgtcccattt
    11761 tgtagttttc tcctttaaat aatgtaccac aacggcctgt acactatagt gtactaccga
    11821 gctggcggat ttgtataaga ttatgctatt aacctattat ttttggttac gacgacgaac
    11881 aataagtttg tctgatttag ctttcttatt acgagtttta agtccaagcg caggtttacc
    11941 ccatggagta gatggtgctt tacgaccaac tggtgcttta ccttcaccac caccgtgtgg
    12001 gtgatcgtta gggttcatta cagaaccacg aactgtaggg cgaacacctt tccaacgatt
    12061 acgtcctgct ttaccaatgt taacaagtga ttgctgttca ttaccaacag taccaattgt
    12121 tgcacggcaa gtaccaagga tcatacgtac ttcacctgat tgaaggcgaa caagaacata
    12181 tttaccttct tgaccaagta cttgagcaga agcaccagcg gcacgaatca attcagcacc
    12241 tttacctggt tgcaattcaa tattatggat aactgtaccg actggaatgt tagcaagcgg
    12301 aagtgcgtta ccaactttga tgtctgcttc tggaccagaa ataatgcgtt gacctacttc
    12361 aaggccttta ggcgcaagga tgtaagcttt aacaccatca gtatagtgta caagagcaat
    12421 atttgcagta cgatttggat cgtattcaat tgttttaaca actgcttcaa caccatcttt
    12481 attacgtttg aagtcaatca aacggtaatg acgtttgtgt ccaccacctt ggtgacgaac
    12541 agtaatacgt ccgttgttgt tacgtcctgc tttattcttt agtgaaacaa gcaatgattt
    12601 ctcaggagtg tttgtagtga tttctgcaaa atccaaagaa gtcatattac gacggccatt
    12661 tgtcgttggt ttataaactt taatacccac gttatttcct ccttagatta ttcagcttca
    12721 gcagcaaaca actcgattgc ttttgaatca gctgtaagag tgataatagc ttttttagtt
    12781 tttgaagtga aacctgtgta acgaccaaca cgttttgctt tgggtttaac tgtcacagtg
    12841 tttacacttg caacttttac tccgtcaaat acagcttcaa cagcttgttt gatcaaaagt
    12901 ttgtgtgcac gtgtgtcaac ttcaaatgta tatttccctg cttcaagagc aaccattgat
    12961 ttctcagtga taacaggttt tttgattacg tcatacaaat tcattatgca agaaccccct
    13021 cgattgtaga gattgcttct ttagtaacaa gaagtttgtc tgcgttaacg atatcaagaa
    13081 cacttgcagt agttgcagta gcaactgtaa cgtttggaag gttacgcgct gaaagtgcag
    13141 caaattcgtt accttcttca aggattacaa gtacttttga atcaatgcta agtgctgaaa
    13201 gaactgacgc aaattcagca gtttttggag ctgcaaatga aaggttttct acagctacaa
    13261 atttatcttc agcaactttt gctgaataaa ctgatttcaa cgcaaggcga cgaacttttt
    13321 gtggaagttt gtagccgtat gaacgtggag ttggtccaaa gacaacacca ccaccacgcc
    13381 attgtggtga gcggatagaa ccttgacgag cgcgtccagt tcctttttga cgccatggtt
    13441 tacgtccacc accagatact gctgaacggt ttttaaccgc gtgagttcct tggcgaaggc
    13501 tagcgcgttg gctgattaca acatcaaaaa caactgattc gtttggttca ataccgaaga
    13561 tagcttcgtt aagctcaact gagctaactt ctttaccagt ttggtcaaat agtttaacgt
    13621 ttgccatttt aactgttttc tcctttctta ttatttagca gctttaactg ctgatttgat
    13681 agtgataaga gatttcttag cacctggtac gttacctttg atgaggacaa cgtttttctc
    13741 tgggataact tgtacaattt caaggttttg aactgttaca cggttaccac ccatacgtcc
    13801 tgccaagcgt ttgtttttga aaacgcggtt aggcgcaaca ggtcccatag aacctggacg
    13861 acgatggtaa cgagaaccgt gagccatagg accacgtgat tgaccatggc gtttgataac
    13921 accttggaaa cctttacctt ttgatgtccc tgtgacgtca acaacatcac cagcttcaaa
    13981 ttgttctaca gataattctg caccaacttc taagccttca atgtttttga attcacgaat
    14041 gaagcgctta ggagctgtgt ttgcttttgc aacatggcct ttggcaggtt tgttactcaa
    14101 tacttcacgt ttgtcatcaa aaccaacctg aactgcttcg taaccgtctg tttcaacagt
    14161 tttaacttga agcacaacgt ttggagttgc ttcaatgaca gtaacaggga tgaattcgcc
    14221 tgattcagtg aagatttgag tcattcccac ttttttccct aagattcctt ttgtcatgag
    14281 aaaatatttc cttttcttat tttatttcaa aaagtttttt atgagcgttt tttgtgctca
    14341 aaaaagtttt ttacgagcgt tttttgtgct cagaaatcaa attaaagttt gatttctacg
    14401 ttgacaccac ttggaagatc aagtttcatc aaagcatcaa cagttttttg tgttgggttg
    14461 atgatgtcta ccaaacgttt gtgtgtacgc atttcaaact gttcgcgaga atctttatat
    14521 ttgtgagtcg cacgaataat tgtgtaaaga ctgcgttcag ttggaagcgg aactggtcca
    14581 gcaactgtag cacctgtacg tgttgcagtt tcaacgattt tttctgccgc tgtatcaagt
    14641 gtacggtgtt cgtacgcttt caaacggata cggatttttt tgtttgccat cttttctcct
    14701 ctcgtctatt tacgataata ggctagctcc tcaagaaaac caacacgagt cgcgtggcaa
    14761 tgcaacctag cgtgtcgcaa cctcttgcat catagctaaa gctgttcata cacagcacca
    14821 gtatatgata tcaaaaaaaa acagcacacg caagcattat tttattattt tcgaaaaaaa
    14881 tgcaaccatt tctggttaca ttttttgaat atatcgatga tagtgaactt tcaaaaacag
    14941 ccacctaaag atattgtctg ctatcgtagc catccaaacc ccaagcaaac ctaattcaaa
    15001 aactattcct aataaataac caagaactac tctgattaac cacatgccta tagtcgttgt
    15061 gtaaaatggg agtttagcat ttcctaatcc ttgccaagct gctgtataaa ccaaggtccc
    15121 aatagtagca ggtactccta cgaaagatag caaaataaca atcaaacttc ctttaatggc
    15181 agaaggatta tttgtaaata actgtgtcaa tggtttgcct gcaagaagca taaaacttga
    15241 tataaggatc attagaacta agccaatata atacgaactt tgaatagttt cttctataga
    15301 ttctctgttt ttttgtccta catattttgc agttaggata attgtagcag ttgcgatgcc
    15361 aagtcctggc atataattaa attgtgtcaa ggtttctcca atagcatttc ccgctacaac
    15421 atttgtacct aactgaacaa caatagcaac tataacaaca tctccagctc gcatcatcag
    15481 acgttctcct gctgatggta aggcaagatt ccaaatttgt gcactgattt tccaagtcat
    15541 tctttttatt atctttttaa taggaagata cttcgcaagg atacaaatac caattaatct
    15601 tgcgataaga gttgatactg ccactccgac caaaccatag cgccactcaa aaatagcaaa
    15661 acctgataag actgcattga ggaaattgac aaaaatactg acatacattg gtaaacgagg
    15721 ttgcccttgc acacgtaaaa atgagcctag ggtggttaac attcctaaag taacaacacc
    15781 tccacccaca attgctaaat ataaaccacc aacttgtgcg acactttttg ttgtccctaa
    15841 aagtttaaag aaagtttgtc caaaaacaat cgagataatt cctaatactg cccctatcag
    15901 taatgttaga aaaatggctt gagaacatac tgaaattgca tcatccttct tactacctgc
    15961 taacgacttg gccaatagac ttgctatact cgcccctaaa gctataaaaa tagcttgata
    16021 aatagtaatt atattattag caactgaaac acctgatact gctacaacac ctaactgagc
    16081 cactagataa ttatcaacta ctcccatgag catttgtaag atattttcaa tcatagctgg
    16141 tatagctaac tgaataatct ctttactata acgcacagta tctcctttct attacccagg
    16201 atcattaaaa agagagtgat gggaaaattt ctaaaatgtt tctttctcgc tctaaatatt
    16261 ttaaaacaat cgtattttta ttattgtttc aaacctagac actcttcaac cgtttctcgc
    16321 atatttgagg taggtactac cgttctatgg attacttcgg ccttttgtag atctgtaacc
    16381 gccttaggta aagaaacacc tgacaaatcg tttaagattg aaatagcagc gaaatcctgt
    16441 atctcttctt tatttgtaat agcctttgta acaacacacg ggaacttata aggacttgct
    16501 gttgacacaa ttactgctgg agtttgatct ttagtttctg tctgataagc ttgataaaca
    16561 gctgatgcta cagctgtatg cggatcctct atatactgat actggtcaaa taaatgtttg
    16621 atgtctaatt caacaaattg ttctgtcgca aatcccgcta caaagctctc taaaatattt
    16681 gcttgcctag cctctaaggc atactcccca gtagtcacta aatcttccat taatttttta
    16741 gtcgtttctg catcatcgcc taaaaggtga aagattaatc gttctagatt agaggagacc
    16801 aaaatatcca tagagggact tgatgtcact ttaaattctc gatttttatc gtaggtttgt
    16861 gtcttaaaaa aatctgttag aacgttatta tcatttgagg cacaaatgag tttggtaaca
    16921 ggtaatccaa tttgactagc ataatatgct gctaaaatat taccaaaatt tcctgtaggt
    16981 acactaaaat taattggttg accaattgag atttcctttg acttaactaa ttgagcatat
    17041 gcatatatat agtaaacaat ttgaggaaca aggcgaccga tgttcattga attggctgat
    17101 gataactgca tatgctgctg tgataatttt aatcgaagaa gggaattatt gaacatttct
    17161 ttaactgaag tctgtgcatc atcaaaattt ccttcaatcg caacaacatg agtattttgt
    17221 ccggcttgtg ttatcatctg caattcttgg atataactaa caccattttt aggataaaag
    17281 acaataatct cagttcctgg aacatctgca aaacctgcca tcgctgcctt gccagtgtca
    17341 cctgatgttg ctgttaaaat aacgatttta ttatcaaccc cttgtttttt ggctgctgtt
    17401 gttagcaaat aaggaagaat ggataaagcc atatctttaa atgcaattgt aggaccgtga
    17461 aataattcaa ggtgataccg gtctccaatc tttactatcg gggctatttc agttgtatca
    17521 aatttagtat cataagcttg actaatacag taatccaatt cttgttcagt aaaatcatca
    17581 aaaaaagctg acaaaacgag tttagccact tcttggtagg aagcatcctt aagtttagtg
    17641 aaatctaaat caacttttgg aaatgtgatg ggggtaaaga gtcctccatc gtcagctaat
    17701 ccctttagaa tcgcttgact agctgtcact ttattactag catcacgtgt tgattgatat
    17761 actattgcca taagaatccc tctttatata aatatctttt agttattgta acacaattta
    17821 ataacatgtc cattgttcgt tatataatta acccccttta ttaaaataaa gggggttcgt
    17881 aacatcatta tcactttgta aagtcaagaa ctttacgtcc ttgaatcaat cctctttcca
    17941 tttcatcgaa tacttgagga gctgtatcca caggcacttt ttcaaccacc ggtactacca
    18001 agccttctgc tccaaatgcg aaggcttctt ctaagtcttt acgagttcca actaaggaac
    18061 caaccacacg aatcccatca agtactgttt ttacaatact caattccata tattctgatg
    18121 gaagaccgac agctaccact gtacctccag cacgaacgct gtcaatcgcc tgattaaacg
    18181 caactttaga aacagcagtc acaaccactc catgacaacc gcctgtcttt tcttgaatat
    18241 atgccgcaac atcttttatt tctttgccat taactgtcaa atctgctcca acctctttag
    18301 ctaattgaag tttatctgcg ttaatatcaa cagctacaac atgagcattg aatacttttt
    18361 ttgcatattg gactgctaag tttccaagac cacctgcacc atacactgca atccactgac
    18421 caggagcagc tccagcttct ttgatagcct tgtatgttgt tactccagca caagtgattg
    18481 atgatgcttg agctgggtct aatccctcag gaaccttaac cgcatagtcc gcggtaacaa
    18541 tagcgtattc actcatacca ccatcaacac tgtatccagc atttttaaca ctacgacaaa
    18601 gtgtctcacg tcctgtagta cagtattcgc aatgaccgca cccttcaaag aaccatgcaa
    18661 tagagacacg atcaccaacc ctcaaagacg ttacgccttc tccaatttct tctactatac
    18721 caatgccttc gtgacccaaa atgcgtcctg gtacctggcc gaaatctcca tgtgcaacgt
    18781 gtaaatcagt atggcataca ccacagtatt ctacttttac aagtgcttca ccatgaccaa
    18841 cttttggaag atcatgctca acaacttcaa cacctgtact agcttgatta acaacaaccg
    18901 ctttcatgat aaaacctcct agaaataaat atatcttatt ttgtaatcgt ttacatttta
    18961 tattgtatta ttcttcacaa actatgtcaa gtgattttca gaaaattttt tgaaaatatt
    19021 cttaaccggt taatcttttt gaaactaaaa atcccactca atagagtgag attcttcgac
    19081 tgctgttact tacgacgtcc tggacgttcc tcataaccat aataagcatc ttcgatgatt
    19141 tcttgcatgt gatctaccat cggaaggcgt ggatttgctg gtgaacattg atcttcatat
    19201 gctaagaaag ctagttcacg agatttttct ttccattctt tttcatcaat gccttggtcg
    19261 cggaaattca tcttaatacc taaacgtgtt ccaaggtcat acacagcttt tgcatatgat
    19321 tcgacagctt cttctggtgt tgctgctggt aagccgagta atttagcgat atcttgatac
    19381 ttttcgtcag cacgatagta attgtactta ggccatgttg ctgttttagc tggacgagtt
    19441 ccattatagc ggataacata cggcaaaaga attgcatttg tacgtccgtg tacggtatgg
    19501 aattgtgcgc caattttatg cgccattgag tgagaaatac ctaagaatgc attggcgaaa
    19561 gccatacctg ccatagttga cgcattgtgc atcttttcac gtgcttcaaa atcagcatct
    19621 tttactgagc gttctaagta gtcaaacaca attttaattg cttgaagagc aagtccgtct
    19681 gtataatcat ttgccatctg tgaaacataa gcctctgttg catgtgtcaa tacatccata
    19741 cccgtatcag ccgcaataaa accaggaaca gtcataacaa gggccggatc aacgatagct
    19801 acagttggtg ttaatgaata atcagcgatt ggatatttac gattatttgc tttgtcagag
    19861 ataacggcaa atggtgtcac ttcagaacct gttccagaag ttgttggaat agcaacaaat
    19921 tttgttttct taccaagttc tgggaatttg aacgcacgtt tacggatatc catgaatttt
    19981 tgaacgaggt catggaaatc aacttctggt tgttcataga agagccacat aactttcgca
    20041 gcatccatag gagaaccacc acctaatgca ataatagtgt ctggtttgaa ggtacgcatc
    20101 aaatctgttc ctttcattac tgttgtaatg tctggatctg gttctacttc agcaaaaatc
    20161 tgataaacaa ctttattgcg acgaagatca agttgttcaa tgatacgatc caagaaacca
    20221 agctcaacca tggcgtgatc tgtaacaatc atgacacgtt caacatcgcg acatttttgc
    20281 aaatattgaa tagaatcacg ttcgaagtat gtttttgaag ggactttaaa ccattgcata
    20341 ttatttctac gtcttcctac ttttttgata ttcaagagat ttatagcact aacgttatca
    20401 ccaactgagt tacgtccata cgaaccacaa ccaagagtca atgatggcaa gaaagcattg
    20461 taaacatccc caataccacc aaaagtagaa ggagaattcc aaataacacg gatagcacga
    20521 attctagttc caaattcacg agctaagtct gcatcttttg tatgaatagc agctgagtgc
    20581 cctagaccat taaactcaac catttgacga gctttttcaa caccatcttc tgtagattca
    20641 gctttcaata ctgcaataac aggtgatagt ttttcacgtg tcaaaggttc tttttcacta
    20701 acttcagtac attctgcggc aagaatattt gttccttctg gaacagtaaa tccagcttgt
    20761 tcagcaatcc aaactgctga ttttccaaca atatttggat ttaattttgc accagcacag
    20821 tttttgctat ttgcttttgc accaaaacag aattcttcaa gaagagcttt ttctttctta
    20881 ttaacgaaat aggtatgata agatttaaat tcttcaacaa attctttata aatttcttta
    20941 tcaataataa cagcctgttc tgatgcacat accataccgt tatcaaatga tttagacata
    21001 acaatatcgt gagcggcttg tcggatatta gctgattttt caacataagc tggaacattc
    21061 ccagcaccta caccaagggc aggtttccca catgagtagg cagctttaac catcgcatta
    21121 ccacctgtcg caaggatagt ggcaatacca tcatggttca tcaaagcatt agttgcatca
    21181 attgatggtt gttcaatcca ttggacacaa ttttcaggag ctccagcagc aatagcagca
    21241 tcacgaacaa tacgtgctgc atgtgctgat gattcttgtg ctgatgggtg gaaggcaaaa
    21301 ataattggat tacgtgtttt aagagagatt aatgacttga aaatagcagt tgaagttggg
    21361 ttggttgttg gcgtgatacc acagacaact cccactggtt cagcaattaa tgtcaagcct
    21421 gtgacatcgt cttcttcaat aactcccact gtctttgtat gacgcatgtt gttaacgacg
    21481 tgttcacatg caaataaatt ttttgtcgct ttatcttcaa agacaccacg accagtttct
    21541 tctactgcat gaagagcaag ttctccatgg gcatccaagg ctgcaacaga agctttagct
    21601 acaatataat caacttgttc ttgattaagt tgacgcatct catcaagtgc ttttaaacca
    21661 ttttgaacca atgtatcaat tgcaagagca acatctgtag tttctactgc tttagttttt
    21721 tcagtcattt gagactcctt ttgatttgtt aaatgtttca caaatttgtt tacgagatta
    21781 ttatagcata tttaaaaact ttgtaaatag tttcacaact tttttgtgaa ctttttttca
    21841 atctttcgga aatatttctt gaaagcgctt taattatatt tgttttttca ttactaattt
    21901 tcagattaaa gcctatcgat ttcattttta tcaccataaa cggagatatc tattattcgt
    21961 tttcacaaaa aaagccgagt acatgactcg gacttgttta ttttgcagct cgttgagctg
    22021 atttttgaat agcttttttg acgttactaa catataaatc agcccctgta gtatcgctat
    22081 cactataatg cacaccatct gtatttcccc agatttctgg atgttctgtc gcaaccttat
    22141 accagtcagc aaccgttatg taattatatt ttttcattag cgagagttca taatcacgca
    22201 ctgtcgtaac ttgactcata tttttagcat tataaggtgt tactataatc aaacggtgtc
    22261 ccttaggcaa agatttaata aattggctca agtctgtctt gtagttatct aatgaattca
    22321 caccaactgc caaaacaaca attttggaca aggctttatt ttgaatacgg ttttcaaaaa
    22381 tatcaaatga tttactaaaa ttacgactca ccgcagcatc taattgtact tctggtaaca
    22441 atttattaaa agcagcactt gaccttaagg caactgaatc tccgattact gtcacatcac
    22501 taagagcacc tgcatcccct gcggctagtg tatgtgttcg gttagtattt gttctagctt
    22561 gttgtaacga attctgcaat aattctgtct caaaactacc tatacttgga gctgtcaaca
    22621 ttgtcactac agtaataaga gttagtaccc ctccgatact aaataaccac tttttgtaag
    22681 gcaacaagtc aaattcataa tcaaggaatt taggttttct acctaaaata aaaggttcaa
    22741 tgatataaaa tgataatgtt gagaagactg ctgataatac tactgtcaaa ataacagcta
    22801 tccagtttgg ggataaacga ctaaaaataa tataaaatgg ccaatgaaaa aggtacatac
    22861 cgtaactgat atcagcaaga taagtaatca caaaaggttc tgaaatatct ggtgtatgct
    22921 cgtgtaaaat acgtgcattg taaatcatta cactagcaaa aatgctagac aatacaaaac
    22981 caaaaagata tgttagccga ttatcaaaat caagtgcaaa agttaaaatc attagaccag
    23041 caaaagcact acccatcatg attaaattat gcttcaaagt cagatttttg atatttttct
    23101 tgaatcgtcc ggtaatctct cgaataccag atattgtagc caccatcgct cctaaaaaga
    23161 acggaaatat atgtgataat gtagagaaat atatcgttga aaaattatca acgaaaaagg
    23221 ctctaacaaa catggtaagg aagctgactc caaaaattcc catggaaata aggaacaaag
    23281 tccccctcaa ttgcttttga tcttttgatc ttttcgataa gagccatacc gttaagcccc
    23341 ataaaacata aaaatggact tcaatagaaa gactccaggt atgcacaaat aagtgaggga
    23401 taaattgact ttcgtagtta ccaccagtca agatttcgta aaagttgctg gtaaaaccta
    23461 aagcagtcat aatttgagag ccaatacttg ctctaaaatc gcttttaacc agaaatacaa
    23521 aaggtattgt caccaaaacc atcaatacta atggtgggaa aatacggtaa aaacgtctac
    23581 gacaaaaact aacaaaatca atttttttcg ttttagaaaa ttcatcaatc aataaggccg
    23641 taatcagaaa accagaaaat gtaaagaaga tgtcaacgcc aacaaatcct cctggaaaac
    23701 tattcttaaa gaaatggtaa agtaaaacaa gtagtagtcc tgttatacgt accaaagaaa
    23761 accattttat tctcattttt gataaatccc ctgtttaaat gtccccttat agactttctt
    23821 atttttagca ttgaggcgac cttgtccatc aggttgacct tttttaaagt cacctgtata
    23881 agaccagcca tgaactgaaa caaatgttcc tttcccttca aagactccat ttacaaaatc
    23941 gcccttatag taatccccat tctcataggt taatttacct ttaccagtca tatggtgatt
    24001 aacaatagag cccacatatt ttatcttgcc gccatcatag ctaaagactc cttgactaga
    24061 agtcgttaag gtaaatactg ataaaccaaa aacaacaata attatcaaag ataatatttc
    24121 tagatgagtt cgtgttattt taaaattctt catttctact taatctctac tattatttat
    24181 tagcgttaac cactgtttac agccatcatt gactagacga taggtttcct caaaatcacc
    24241 agtataccat ggatcaggaa caccaccatc acaaaaaaga ctaatcttat catcataggt
    24301 tccttgtgat agagctttta aatccttgac attttgctca tccataccga tgatataatc
    24361 aaaatatgct aaatcgtctt tagaaatagg ttgtgaccct ttatcgttaa tgtaaggaat
    24421 ttgataacgc tgcaaaatcg cttgcgttcc cctatgtata ggatttccat gctcccatgt
    24481 tgaagtcgca cgactctcaa ccaagaggct atcctcctta tctaaattcg ccataacaaa
    24541 ctcagccatc gggctacggc agatatttcc aagacaaaca aaacagactt tcttcatagc
    24601 aaactccttt tggtctatta taacagaaaa aaggtactta taatacgttt aactgctcaa
    24661 agaataacta caacatatct aatacttaac attgcatgaa aattaaaaaa ttaagactag
    24721 atactctagc cttaaaatga cttatgtttt atttatcaaa tcgctgataa cctaaatgtt
    24781 catatgcctt aaccgtagct acacgaccgg tacgggtacg cataataaaa cctttttgaa
    24841 ttaaataagg ttcgtacatg tcttcaacag tatctcgttc ttcagcaata ttaacggata
    24901 gagttcctaa accaacagga cctccattat acatttcaat catggttctt aagatttttt
    24961 gatcgacgta atcaagcccc tcgtgatcaa catctaacat cgttaatgct ttatctgtaa
    25021 tattgtcatc tatcaaacca tctcccatga tttgagcata atctcgaaca cgtttcaata
    25081 aacggttagc gatacgtggc gttccacgac tgcgacgcgc taattcagaa gcagcttcat
    25141 aagtaatttt catttcaaaa atgtctgctg tacgctcaat aatttctgtc aaatcatttt
    25201 cttcataata ctccatatgc cctgtaatac caaagcgagc acgtaaggga ttagataaca
    25261 tacctgcacg tgtcgttgca ccaattaagg taaagggcgg caaatctaga tgaacacttc
    25321 tactagtttc tcctgcaccg atcataatgt caatatagaa gtcttccatt gcactataaa
    25381 gcacctcttc aaccgccatc ggcatacgat gaatttcatc aataaaaaga acatcacctg
    25441 gttctaaatc atttaaaatg gctactaaat cccctgattt ttcaattgcg ggacctgatg
    25501 tttgtttgag attgacaccc aactcattag caattacaaa tgccatggtt gttttcccta
    25561 aaccaggagg gccaaataat aacacatgat ccaatgactc atcacgcaat ttagcagctt
    25621 caataaatat ttttagctga tctttaacct tatcttgtcc aatatactct cttaaatact
    25681 gcggacgaag tgtacgttct accaattctt cgtcacccat tgcatcacta tctaaaaatc
    25741 ttgtcatgat attattataa catttgtata ggaaaagtta agaactaagt tggtgtatta
    25801 gtatctccct tatttaaagg aagaataaac tgatcttata gttaatccaa cctcaagaaa
    25861 tcacagaaca gcttacttag cccatattcc accaacccat tcctaaaaac aaatcaaatg
    25921 aacctaaatt tgataataga acaagtaata ctattaaaaa acattttttt atcactataa
    25981 taaactccta aataatattt tgtacgttta ttataaattt ttagacctca gagtcacagg
    26041 acatcaatgt cctgttatat cttcttgcca ctccaattct aattttttaa ttagatgctt
    26101 gtcatcaatc atttttgaaa acataattga ttgttggtaa gcattttctg cagactttat
    26161 atcattgtca attgtaatga agtatttcca ttggtacata aaatataaag gcattttatg
    26221 aaaatcatgt gttgacacca tcgtttgtcg gctaacttct aacatttctg caagacactc
    26281 ttcactattt aaacaaagtc caacaaatac acaattcagg agtacatgat taaaccaaaa
    26341 caaatcttct gctgtaagtt tgtggttctg acttatcaaa gttttacata tagtttgaaa
    26401 tacttcttta gtaaatgctc tattgttaaa tttagataca actgcacaag ttaaaaacaa
    26461 atctatgata atcaaatcat taagagtata tttttcttta tattttactt tatcaaagca
    26521 ttcgcataaa acctccacac caaaattaat gtctcctgtt tgatagactt caaatttagc
    26581 ttgtaaactg tcaattgcta agcactcgtc ttctggaaga ttatcataga attcttcgaa
    26641 aatatgatca aactgacatt ctcttaattt taatcgttca gcatctgcat aagttggaat
    26701 tcttaaaatc aaatacttca actctttata ccgtttaggt aattctaagc tctctcctcc
    26761 tgttaaaata tccaatttta cattcaattg tttagctatg tagtgagctt tagtcaagtt
    26821 tggaattgac tgtccacttt caatcctagc taattgtctt acagataact cagtttcatc
    26881 accacaaaaa tcttcgcgag taatatttct agttactctc aaatttctta ctttactacc
    26941 aaaatgtttt aacattttcc tttaaaaact aactcctaaa tttttcttct tattatatat
    27001 tatcataata aactcatttt aagtatgttt tttaatatta attcctttta aatcaataaa
    27061 aattccatta ctggttaaaa aaacgttaac cagtaatgga attttcttat ttttctaatc
    27121 ctagtctttc aaaaatatca tcaacacgtt ttgtgtaata aatcggatta aatagttcat
    27181 caatttcttc ctgtgtaaga caagaggtaa ctttggtgtc ttcttctaaa agtggtttga
    27241 aatccacttg attgtcccag gaataagctg tcttaggttg aactaagtca taagcttctt
    27301 ctcgtgtcat tcctttttca atcaatttga gcataacacg ttgactataa atcaaaccaa
    27361 aagttgattc catattgcgc atcatatttt ccgggaagac agtcaagttc ttaacgatat
    27421 tgccaaaacg gttgagcatg tagtcaatca agatcgttgt gtcaggtgtg atgatacgct
    27481 cagcagatga gtgcgaaata tcacgctcgt gccaaagtgc cacgttctca taagccgtca
    27541 ccatgtgacc acgaatcacg cgcgctagcc cagtcatgtt ctctgaaccg attgggttgc
    27601 gtttgtgagg catagcagag ctacctttct gacctttggc aaagaattct tcaacttcac
    27661 gttgttctga tttttgcaga ccacgaatct ctgtcgccat acgttcgata gaagttgcaa
    27721 tgcttgcaag cactgcaaaa tattctgcgt ggaggtcacg tggaagaacc tgtgttgaaa
    27781 tctcttgcgg acggatgccc aatttgtcac aaacatattg ttccacaaat ggaggaatgt
    27841 tagcaaagtt accaacggca cctgaaatct taccagcttc cacacctgcg gcagcatgtt
    27901 caaaacgctc aatattacgt ttcatctcgc tgtaccaggt cgccaactta agaccgaaag
    27961 tcgttggctc agcgtgaaca ccgtgggtac gacccatcat gatggtgaac ttgtgctcct
    28021 tagccttatc agccacaata tttgtgaaat tctcaaggtc acgacggata atatcgttag
    28081 cctgcttgta gaggtaaccg taggcagtgt ccaccacgtc agtcgacgtc aaaccgtagt
    28141 gcacccactt gcgctcctca ccaagcgtct cagaaaccgc acgagtgaaa gccaccacat
    28201 cgtgacgcgt gtcctgctca atctcaagaa tgcggtcaat gtcaaaatcc gccttctcac
    28261 gaatcttagc cacatcctcc ttaggaatct cacccaactc agcccatgcc tcgtcagcca
    28321 aaatctcgac ctccaaccaa gcacggtatt tattttcctc tgtccaaatt gccgccatct
    28381 cagggcgtga ataacgttcg atcattttgt tttactcctt taaaaaattc taattactac
    28441 ccttagcttt atcatattct ttttcaaaat aattcaaaga ttcttcagtt aaataatccg
    28501 acacttcttt taatatttct ttagcttttt caacttctcc taaagaaatt aagatgtata
    28561 tgtattcctc ataaagttta ggtataaat
//
LOCUS       DATRMA010000019        28211 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.019, whole genome shotgun sequence.
ACCESSION   DATRMA010000019 DATRMA010000000
VERSION     DATRMA010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 28211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28211
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.019"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            complement(<1..154)
                     /locus_tag="VBC28_000795"
     CDS             complement(<1..154)
                     /locus_tag="VBC28_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HEO6814796.1"
                     /translation="MSTLTIIIATLTALEHFYIMYLETLATQSNMTGKIFSMSKEELS
                     YLPVIKL"
     gene            complement(221..2539)
                     /locus_tag="VBC28_000796"
     CDS             complement(221..2539)
                     /locus_tag="VBC28_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000883428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HEO6814797.1"
                     /translation="MLDELKAIIKSPKLWITMAGVALIPTLYNVIFLSSMWDPYGNTK
                     NLPVAVVNQDKSAKLNGKTISIGKDMEDNLSKNDSLDFHFTTAKRAEKELEKGHYYMV
                     ITFPKDLSRKATTLMTEKPERLNITYKTTKGRSFVASKMSETAANKLKDEVAESITGT
                     YTESVFKNMGSMKTGINKAADGSQELLNGSNKLQDGSQTLTSNLDVLASSSQTFSGGA
                     NKLNSGINLYTDGVGTLSNGLETLSDGVTAYTTGVHKLSEGSQKLDDKSQALVEGSEK
                     LTDGLQQLSQATQLKPEQERTLQNLSDGLKNLNQIITNLQSTATTDSDTNSKLFNFLS
                     TIESSTKALMNTAAADKQKQMTAVQSTSAFKSLTPEQQSQITSAVTGTPTSAETIAAN
                     ISSNIENMKTVLSEASSSAPSNNGSQNLQTLSGTANNLVLKAISDLDKIQKLPTATKQ
                     LYQGSQTLTKGITDYTNAVGQLRKGAVTLDSKSNQLISGTQKASQGAQTLDSKSDQLR
                     DGAGQLASGSDRIADGSNKLAGGGHQLTDGLTELSGGVSQLSSSLGKAGDQLSMVSVN
                     KDNANAVSSPVTIKHEDYDSVDTNGVGMAPYMISVALMVVALSANVIFAKALSGKEPA
                     NRFSWAKNKLLINGFIATLAATILFFAVQFIGLKPDYPGKTYFIILLTAWTLMALVTA
                     LVGWDNRYGSFLSLLILLFQLGSSAGTYPIELSPKFFQTIQPFLPMTYSVSGLRETIS
                     LTGDVNHQWRMLVIFLVSSMILALLIYRKQED"
     gene            2678..3217
                     /locus_tag="VBC28_000797"
     CDS             2678..3217
                     /locus_tag="VBC28_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HEO6814798.1"
                     /translation="MVNDTRREKTKRAIEAAMITLLKDQSFDEISTINLTKTAGISRS
                     SFYTHYKDKYEMIDQYQQSLFNKVEYIFDRNQFKKEDALLEIFQFLDRESLFAALLTQ
                     NGTKEIQTYILNKLQLMLSKELPVVNPDATKSDINRLYYSVYLSHAIFGVYQMWITRG
                     KKESPQQITQVLLSLLPQT"
     gene            complement(3303..3599)
                     /locus_tag="VBC28_000798"
     CDS             complement(3303..3599)
                     /locus_tag="VBC28_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4298 domain-containing protein"
                     /protein_id="HEO6814799.1"
                     /translation="MNLNDRLKIEEMEEKYDSFKPRINALVEAIDDFQKHYEDYVKLR
                     EFYGSEDWFRLSEQTENNLKCGVLSEDQLFDFIGEHNELVGQFLDMSSQMYRHL"
     gene            complement(3843..4454)
                     /gene="rpsD"
                     /locus_tag="VBC28_000799"
     CDS             complement(3843..4454)
                     /gene="rpsD"
                     /locus_tag="VBC28_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEO6814800.1"
                     /translation="MSRYTGPSWKQSRRLGLSLTGTGKELARRNYVPGQHGPNNRSKL
                     SEYGLQLAEKQKLRFSYGLGEKQFRNLFVQATKAKEGTLGFNFMVLLERRLDNVVYRL
                     GLATTRRQARQFVNHGHILVDGKRVDIPSYRVTPGQVISVREKSMKVPAILEAVEATL
                     GRPAFVSFDAEKLEGSLTRLPERDEINPEINEALVVEFYNKML"
     gene            complement(4784..5071)
                     /locus_tag="VBC28_000800"
     CDS             complement(4784..5071)
                     /locus_tag="VBC28_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HEO6814801.1"
                     /translation="MSDAFADVAKMKKIKEDIKSHEGQMVELTLENGRKREKNKIGRL
                     IEVYPSLFIVEYKDTAAVPGAIDNTYVESYTYSDILTEKTLIRYFDDESAE"
     gene            complement(5083..6438)
                     /gene="dnaB"
                     /locus_tag="VBC28_000801"
     CDS             complement(5083..6438)
                     /gene="dnaB"
                     /locus_tag="VBC28_000801"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEO6814802.1"
                     /translation="MVEVSELRVQPQDLLAEQAVLGSIFISPEKLIMVREFISPDDFY
                     KYSHKVIFRAMITLADRNDAIDAATVRNILDDQGDLQNIGGLGYIVELVNSVPTSANA
                     EFYAKIVSEKAMLRDIISKLTDTVNMAYEGNDSDEIIATAEKALVDINEHSNRSGFRK
                     ISDVLKVNYENLELRSQQTSDVTGLPTGFRDLDRITTGLHPDQLIILAARPAVGKTAF
                     VLNIAQNVGTKQNRPVAIFSLEMGAESLVDRMLAAEGMVDSHSLRTGQLTDQDWNNVT
                     IAQGALADAPIYIDDTPGIKITEIRARSRKLSQEVDDGLGLIVIDYLQLISGTRPENR
                     QQEVSEISRQLKILAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYRREGEEAEEIVEDNTVEVILEKNRAGARGTVKLMFQKEYNKFSSIAQFE
                     E"
     gene            complement(6481..6933)
                     /gene="rplI"
                     /locus_tag="VBC28_000802"
     CDS             complement(6481..6933)
                     /gene="rplI"
                     /locus_tag="VBC28_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEO6814803.1"
                     /translation="MKVIFLQDVKGKGKKGEVKEVPTGYAQNFLLKKNLAKEATTQAI
                     GELKGKQKSEEKAQAEILAQAKELKTQLESETTRVQFIEKVGPDGRTFGSITAKKIAE
                     ELQKQYGIKIDKRHIDLDHTIRAIGKVEVPVKLHKQVSSQIKLDIKEA"
     gene            complement(6939..8921)
                     /locus_tag="VBC28_000803"
     CDS             complement(6939..8921)
                     /locus_tag="VBC28_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEO6814804.1"
                     /translation="MKRFRFATVHLVLIGLILFGLLAICVRLFQSYTALLLAIFVALS
                     FVVALLYYQKITYELSEVEQIELLNDQTEVSLKSLLEQMPVGVIQFDLETNDIEWFNP
                     YAELIFTGDNGHFQSATVKDIITSRRNGTAGQSFEYGDNKYSAYLDTETGVFYFFDNF
                     MGNRRNYDSSMLRPVIGIISIDNYDDIMDTMLEADMSKINAFVTSFISDFTQSKNIFY
                     RRVNMDRYYIFTDYSVLNTLIKDKFDILNEFRKRAQENHLSLTLSMGISYGDGNHNQI
                     GQIALENLNTALVRGGDQIVVRENDSSKKALYFGGGAVSTIKRSRTRTRAMMTAISDR
                     LKVVDSVFIVGHRKLDMDALGASVGMQFFASNIVNASYVVYDPNDMNSDIERAIDYLQ
                     EDGETRLVSVERAFELITQNSLLVMVDHSKTALTLSKEFFNKFADVIVVDHHRRDEDF
                     PKNAVLSFIESGASSASELVTELIQFQQAKDKLSRSQASILMAGIMLDTRNFASNVTS
                     RTFDVASYLRGLGSNSMAIQKISATDFDEYRLINELILKGERIYDNIIVATGEEHKVY
                     SHVIASKAADTMLTMAGIEATFVITKNSSNIGISARSRNNINVQRIMEKLGGGGHFSF
                     AACQIQDKSVKQVRRMLLEIIDEDLRENSTVENRRD"
     gene            complement(8988..10889)
                     /gene="mnmG"
                     /locus_tag="VBC28_000804"
     CDS             complement(8988..10889)
                     /gene="mnmG"
                     /locus_tag="VBC28_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEO6814805.1"
                     /translation="MTHNFAENYDIIVVGAGHAGVEASLAASRMGCKTLLATINLEML
                     AFMPCNPSIGGSAKGIVVREIDALGGEMGKNIDKTYIQMKMLNTGKGPAVRALRAQAD
                     KALYAQTMKQTVEKQENLTLRQAMIDEILVEDGKVVGVRTATNQKFSAKSVVITTGTA
                     LRGEIILGDLKYSSGPNNSLASVTLADNLRDLGLEIGRFKTGTPPRVKASSINYEKTE
                     IQPGDEQPNHFSFMSRDEDYITDQVPCWLTYTNTLSHDIINQNLHRAPMFSGIVKGVG
                     PRYCPSIEDKIVRFADKERHQLFLEPEGRYTEEVYVQGLSTSLPEDVQVDLLRSIKGL
                     ENAEMMRTGYAIEYDIVLPHQLRATLETKVIAGLFTAGQTNGTSGYEEAAGQGLVAGI
                     NAALKVQGKPELILKRSDAYIGVMIDDLVTKGTLEPYRLLTSRAEYRLILRHDNADMR
                     LTEIGYEIGLVDEERYAIFKKRQMQFENELERLDSIKLKPVSETNKRIQELGFKPLTD
                     ALTAKEFMRRPQITYAVATDFVGCADEPLDSKVIELLETEIKYEGYIKKALDQVAKMK
                     RMEEKRIPPHIDWDDIDSIATEARQKFKKINPETLGQASRISGVNPADISILMVYLEG
                     RQKGRKNIN"
     gene            complement(11059..11670)
                     /locus_tag="VBC28_000805"
     CDS             complement(11059..11670)
                     /locus_tag="VBC28_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000623753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HEO6814806.1"
                     /translation="MISKFILAFMAFFAIMNPISNLPAFMALVADDDQKISRRIAAKG
                     VLLAFVIIVIFVLSGHLLFNLFGITLAALKISGGILVGIIGYKMINGIHSPTNKNLEE
                     HKDDPMNVAVSPLAMPLLAGPGTIATAMGLSSGGLSGKLITILAFAILCVIMYVILIS
                     ANEITKFLGKNAMTIITKMMGLILMTIGIEMLITGIKIGFHLT"
     gene            complement(11702..12823)
                     /gene="mnmA"
                     /locus_tag="VBC28_000806"
     CDS             complement(11702..12823)
                     /gene="mnmA"
                     /locus_tag="VBC28_000806"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEO6814807.1"
                     /translation="MTDNSNIRVVVGMSGGVDSSVTALLLKEQGYDVIGVFMKNWDDT
                     DEFGVCTATEDYKDVAAVADQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMTLGADYVATGHYAQVTRDENGIVHMLRGADNNKDQTYFLSQLS
                     QEQLQKTLFPLGHLQKPEVRRIAEEAGLATAKKKDSTGICFIGEKNFKDFLGQYLPAQ
                     PGRMMTVDGRDMGEHAGLMYYTIGQRGGLGIGGQHGGDNKPWFVVGKDLSKNILYVGQ
                     GFYHDSLMSTSLTASEIHFTRDMPNEFKLECTAKFRYRQPDSKVTVYVKGNQARVVFD
                     DLQRAITPGQAVVFYNEQECLGGGMIDQAYRDDKICQYI"
     gene            13069..13737
                     /gene="sdaAB"
                     /locus_tag="VBC28_000807"
     CDS             13069..13737
                     /gene="sdaAB"
                     /locus_tag="VBC28_000807"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HEO6814808.1"
                     /translation="MKHLKFQSVFDIIGPVMIGPSSSHTAGAVRIGKVVHSIFGEPSE
                     VTFHLYNSFAKTYQGHGTDKALVAGILGMDTDNPDIKNSLEIAHQKGIKIYWDILKDS
                     NSPHPNTAKITVKNGDRSMSITGVSIGGGNIQVTELNGFSVSLTMNTPTLIIVHQDIP
                     GMIAKVTDILSDFNINIAQMNVTRESAGEKAIMIIEVDSRDCQQAVKKIEAIPHLHNV
                     NFFD"
     gene            13752..14624
                     /gene="sdaAA"
                     /locus_tag="VBC28_000808"
     CDS             13752..14624
                     /gene="sdaAA"
                     /locus_tag="VBC28_000808"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HEO6814809.1"
                     /translation="MFYTIEELVEQANSQHKGNIAELMIQTEIEMTGRSREEIRYIMS
                     RNLEVMKASVIDGLTPSKSISGLTGGDAVKMDQYLQSGKTISDTTILAAVRNAMAVNE
                     LNAKMGLVCATPTAGSAGCLPAVISTAIEKLNLTEEEQLDFLFTAGAFGLVIGNNASI
                     SGAEGGCQAEVGSASAMAAAALVMAAGGTPFQASQAIAFVIKNMLGLICDPVAGLVEV
                     PCVKRNALGSSFALVAADMALAGIESQIPVDEVIDAMYQVGSSLPTAFRETAEGGLAA
                     TPTGRRYSKEIFGE"
     gene            complement(14759..15463)
                     /locus_tag="VBC28_000809"
     CDS             complement(14759..15463)
                     /locus_tag="VBC28_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEO6814810.1"
                     /translation="MNKRRKLSKLNVKKHHLAYGAITLVALFSCILAVMVIFKSSQVT
                     TESLSKADKVRVAKKSKMTKATSKSKVEDVKQAPKPSQASNEAPKSSSQSTEANSQQQ
                     VTASEEAAVEQAVVTENTPATSQAQQAYAVTETTYRPAQHQTSGQVLSNGNTAGAIGS
                     AAAAQMAAATGVPQSTWEHIIARESNGNPNVANASGASGLFQTMPGWGSTATVQDQVN
                     SAIKAYRAQGLSAWGY"
     gene            complement(15569..16108)
                     /locus_tag="VBC28_000810"
     CDS             complement(15569..16108)
                     /locus_tag="VBC28_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEO6814811.1"
                     /translation="MSITSVKKSKPFKLGVAGLLVGASLALPLSVSAASYTVKSGDTL
                     SAIAKNHKTTVQELVSLNSISNADVISIGDVLKLDNSTASQAEAKSQPTIENSMNSSS
                     NLSSSDSAAKEEIARRESNGSYTAQNGQYYGRYQLSQSYLNGDLSPENQEKVADNYVV
                     SRYGSWSAALSFWNSNGWY"
     gene            complement(16324..17118)
                     /locus_tag="VBC28_000811"
     CDS             complement(16324..17118)
                     /locus_tag="VBC28_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEO6814812.1"
                     /translation="MDKLILGRYIPGNSLIHKLDPRSKLLAMLLFIIIVFWANNVVTN
                     VIVFIFTLVIVGLSQIKFSYFFNGIKPMVGIILFTTLFQMLFAQGGQVIFSFWIFSIT
                     SLGLQQAALIFMRFVLIIFFSTLLTLTTTPLSLADAVESLLKPLEVLRVPAHEIGLML
                     SLSLRFVPTLMDDTTRIMNAQRARGVDFGEGNLIHKVKSIIPILIPLFASSFKRADAL
                     AIAMEARGYQGGANRSKYRLLKWTVRDTFSILLMLLLGLSLFLLKN"
     gene            complement(17111..17953)
                     /locus_tag="VBC28_000812"
     CDS             complement(17111..17953)
                     /locus_tag="VBC28_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000510607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HEO6814813.1"
                     /translation="MGIEFKNVSYTYQAGTPFEGRALFDVNLKIEDASYTAFIGHTGS
                     GKSTIMQLLNGLHIPTKGEVIVDDFSIKAGDKNKEIKFIRQKVGLVFQFPESQLFEET
                     VLKDVAFGPQNFGISQIEAERLAEEKLRLVGISEDLFDKNPFELSGGQMRRVAIAGIL
                     AMEPKVLVLDEPTAGLDPKGRKELMTLFKNLHKKGMTIVLVTHLMDDVADYADYVYVL
                     EAGKVTLSGQPKQIFQEVELLESKQLGVPKITKFAQRLSHKGLNLPSLPITINEFVEA
                     IKHG"
     gene            complement(17929..18768)
                     /locus_tag="VBC28_000813"
     CDS             complement(17929..18768)
                     /locus_tag="VBC28_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HEO6814814.1"
                     /translation="MTNIITVNNLFFKYDSNQTHYQLENVSFHVKQGEWLSIIGHNGS
                     GKSTTVRLIDGLLEAESGQIIIDGQELTEDNVWELRHKIGMVFQNPDNQFVGATVEDD
                     VAFGLENKGIPLKDMKERVDQALDLVGMSEFKMREPARLSGGQKQRVAIAGAVAMRPQ
                     VIILDEATSMLDPEGRLELIRTIRAIRQKYNLTVISITHDLDEVALSDRVIVMKNGKV
                     ESTSTPKALFGRGNRLISLGLDVPFTSRLMAELAANGLDIGTEYLTEKELEEQLWELN
                     LKM"
     gene            complement(18768..19310)
                     /gene="pgsA"
                     /locus_tag="VBC28_000814"
     CDS             complement(18768..19310)
                     /gene="pgsA"
                     /locus_tag="VBC28_000814"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003090075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEO6814815.1"
                     /translation="MMKKENIPNLLTVVRILMIPLFIVLTSVTTSTTWHIVAAIVFAI
                     ASLTDYLDGYLARKWQVVTNFGKFADPLADKMLVMSAFIMLVGLDLAPAWVSAIIICR
                     ELAVTGLRLLLVETGGTVLAAAMPGKIKTATQMFAVIFLLVHWMTLGNIMLYIALFFT
                     LYSGYDYFKGAGFLFKDTFK"
     gene            complement(19433..20716)
                     /locus_tag="VBC28_000815"
     CDS             complement(19433..20716)
                     /locus_tag="VBC28_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000217763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEO6814816.1"
                     /translation="MTVNKITYQNLQEEVYKLTLESGLNVYLIPKPSFKETVGVLTAN
                     FGSLHTKYTRNGCVEHYPAGIAHFLEHKLFELDKGQDAATQFTKYGAESNAFTTFDKT
                     SFYFSTISHITNCLDILLDFVLTTNFTEESITKEKDIIKQEIEMYQDDPEYRLYQGVL
                     SNLYPNSPLAFDIAGDYQSISQITLTDLQENHKDFYQLSNMNLVLVGQFSPQEIITYL
                     QKNSHFTSYSQNIDRDSISLEPVIKNNSCHMTVTKPKLAIGYRKSNHMIHGSYLKEKI
                     GLQLFFAMLLGWTSTINQDWYESGQIDDSFDIEIEVHPDFECVIISLDTTEPIAFSTQ
                     LRLLLKNALQSSDLTESHLKNVKRELYGDFLRSLDSIENLAMQFVTYLYDGKTMYLDL
                     PSIVEELDLEDVITIGKDFLDNADTSDFVIFPKSS"
     gene            complement(20718..21962)
                     /locus_tag="VBC28_000816"
     CDS             complement(20718..21962)
                     /locus_tag="VBC28_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEO6814817.1"
                     /translation="MKIVEGVSLHLIKNQQFKTNHLTFRFSGDFNNKTVARRSLVAQM
                     LVTANAKYPKVQEFREKLASLYGASLSTKISTKGLVHIVDIDIVFVKNTFTLEQENIV
                     EQIITFLEDMLFSPLISLEQYQTSIFDTEKKNLIQYLEADIEDNFYSSDLALKSLFYN
                     NKTLRLPKYGTASLVESENSFTAYQEFQKMLKEDQLDIFVVGDFDDYRMIQAFNRMAF
                     EPRHKVLAFDYTQTYENITRSQVEDKDVNQSIMQLAYHLPITYKDEDYFALIVFNGLF
                     GAFAHSLLFTEIREKQGLAYTIGSQFDSFTGLFTIYAGIDKENRERFLKLINKQFNNI
                     KMGRFSSTLLKQTKDILKMNYVLASDNPKVIVDHIYHEHYLDQFHTSALFIDKVDDVT
                     KSDIVSVATKLKLQAFYFLEGN"
     gene            22201..22554
                     /gene="yaaA"
                     /locus_tag="VBC28_000817"
     CDS             22201..22554
                     /gene="yaaA"
                     /locus_tag="VBC28_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000384871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HEO6814818.1"
                     /translation="MDYKLFDEYITLQSLLKEIGIIQSGGAIKKFLADNRVLFNGDLE
                     NRRGKKLRLGDIITIPDQNIEIIIRKPSDQEIEERNIEIAEKQRVSAIVKEMNKNTNK
                     GKSKTSKKPVRFPGT"
     gene            22557..23666
                     /gene="recF"
                     /locus_tag="VBC28_000818"
     CDS             22557..23666
                     /gene="recF"
                     /locus_tag="VBC28_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEO6814819.1"
                     /translation="MWIKNISLKHYRNYEEAQVDFSPNLNIFIGRNAQGKTNFLEAIY
                     FLALTRSHRTRSDKELVHFKHHDVQITGEVIRKSGHLNLDIQLSEKGRITKVNHLKQA
                     KLSDYIGAMTVVLFAPEDLQLVKGAPSLRRKFLDIDIGQIKPTYLAELSNYNHVLKQR
                     NTYLKTTNNVDKTFLTVLDEQLADYGSRVIEHRFDFIQALNDEADKHHYIISTELEHL
                     SIHYKSSIEFTDKSSIREHFLNQLSKSHSRDIFKKNTSIGPHRDDITFFINDINATFA
                     SQGQQRSLILSLKLAEIELIKTVTNDYPILLLDDVMSELDNHRQLKLLEGIKENVQTF
                     ITTTSLEHLSALPDQLKIFNVSDGTISINEKKATD"
     gene            complement(23678..24544)
                     /locus_tag="VBC28_000819"
     CDS             complement(23678..24544)
                     /locus_tag="VBC28_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HEO6814820.1"
                     /translation="MEGLLIALIPMFAWGSIGFVSNKIGGRPNQQTFGMTLGALLFAI
                     IVWLFKQPEMTASLWIFGILGGILWSVGQNGQFQAMKYMGVSVANPLSSGAQLVGGSL
                     VGALVFHEWTKPIQFILGLTALTLLVIGFYFSSKRDVSEQALATHQEFSKGFATIAYS
                     TVGYISYAVLFNNIMKFDAMAVILPMAVGMCLGAICFMKFRVNFEAVVVKNMITGLMW
                     GVGNVFMLLAAAKAGLAIAFSFSQLGVIISIIGGILFLGETKTKKEQKWVVMGILCFV
                     MGAILLGIVKSY"
     gene            complement(24600..25262)
                     /locus_tag="VBC28_000820"
     CDS             complement(24600..25262)
                     /locus_tag="VBC28_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001868421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO6814821.1"
                     /translation="MKNYGKILKELREDKGISLSSLAKSAQLSKSTLSRFENGETQIG
                     IDKFIKALQTLEVGVTINEVSILDSKVKAGTSNTDLEQLTLLESYRDNEDIMRIFSFQ
                     KQQSCDRIESNVLKILAKLFISNLGLNMRLPQDEINLVVTYLNGVTQYNDFYFKVICY
                     FQDILPEDVILNKISNMTKEQLPYSKSLVNLLIKQVIIALEKDSVDKAIVFADLANKV
                     SQ"
     gene            complement(25337..26818)
                     /gene="guaB"
                     /locus_tag="VBC28_000821"
     CDS             complement(25337..26818)
                     /gene="guaB"
                     /locus_tag="VBC28_000821"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012516513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEO6814822.1"
                     /translation="MSNWDTKFLKKGFTFDDVLLIPAESHVLPNEVDMKTKLADNLTL
                     NIPIITAAMDTVTDSKMAIAIARAGGLGIIHKNMSIVDQAEEVRKVKRSENGVIIDPF
                     FLTPDNTVSEAEELMQNYRISGVPIVETLENRKLVGIITNRDMRFISDYKQLISEHMT
                     SQNLVTAPIGTDLETAERILHEHRIEKLPLVDDEGRLSGLITIKDIEKVIEFPKAAKD
                     EFGRLLVAGAVGVTSDTFERAEALFEAGADAIVIDTAHGHSAGVLRKIAEIRAHFPNR
                     TLIAGNIATAEGARALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQITAIYDAAAVAR
                     EYGKTIIADGGIKYSGDIVKALAAGGNAVMLGSMFAGTDEAPGETEIFQGRKFKTYRG
                     MGSIAAMKKGSSDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIVFQMLGGIRSGMGY
                     VGAANIKELHDNAQFVEMSGAGLKESHPHDVQITNEAPNYSVH"
     gene            complement(26975..27460)
                     /locus_tag="VBC28_000822"
     CDS             complement(26975..27460)
                     /locus_tag="VBC28_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEO6814823.1"
                     /translation="MNKKETRHQLIRSLVSETKVRTQHELRELLEKNGVSVTQATLSR
                     DMKELNLIKVNESSDNATETYYEIHSISQKRWEERLRFYMEDALVMLFPVQNQIVLKT
                     LPGLAQSFGSILDSILLPEILATVCGDDTCLIICNNSEEAQKCFEKLSQYTPPFFFSK
                     K"
     gene            complement(27470..28150)
                     /locus_tag="VBC28_000823"
     CDS             complement(27470..28150)
                     /locus_tag="VBC28_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEO6814824.1"
                     /translation="MITKEQYFYFRQLDDFKHFTIEQFDHIVSHIKHRTALKNHTLFF
                     EGDYREKLFLIQSGHVKIEQSDASGSFIYTDYVRQGTVFPYGGLFLDDDYHFSAVAIT
                     DIEYFSLPMALYEEYSLQNINQMKHLCRKYSKLLRVHEIRLRNMVTSSASMRVIQSLA
                     TLLLQVPTERGHLPFPITTIEIANMSGTTRETVSHVLKELRQKDIVEMKGKKLLYNNK
                     NYFKKFIE"
BASE COUNT         9137 a         5432 c         4372 g         9270 t
ORIGIN      
        1 aaagtttaat aacgggtaaa tatgacaact cttctttaga catactaaaa atcttcccag
       61 tcatatttga ctgggtggct aacgtctcca aatacataat ataaaaatgt tccaaagcag
      121 ttaatgttgc aataattatt gttaaagtag acatatcttc tcctcctaaa tactttgaca
      181 taccatatca tactgttagt ctatcactag atactttcta ttaatcttct tgtttacgat
      241 aaataagaag agcaagtatc atcgatgata ctaaaaagat tactagcatt ctccattgat
      301 ggttaacgtc tcccgtcaac gagatggtct ctcttaatcc tgaaacagag taagtcatcg
      361 gtaaaaatgg ttgaattgtt tgaaagaact taggactcaa ttctattggg taagttcctg
      421 cgcttgaacc aagctggaat aataatatta ataacgacaa gaaggaacca tacctattat
      481 cccatcccac taaagcagtt actaatgcca tcaaagtcca tgctgtcaat aggataataa
      541 agtaggtttt tccagggtaa tctggtttaa gacctatgaa ttgaactgca aaaaataaga
      601 tagttgctgc tagagttgca atgaatccat tgattaataa tttattttta gcccaactaa
      661 aacgattagc tggctctttg ccagataaag ctttcgcaaa aataacattg gctgataaag
      721 ctaccaccat caacgcaact gaaatcatgt atggtgccat tccaacacca ttcgtatcaa
      781 cgctatcata atcttcatgc ttgatagtga ctgggctaga tactgcattg gcattatctt
      841 tatttacaga aaccatggac aactggtccc cagctttccc caaagaactt gataattgag
      901 aaacaccacc tgacaactct gtcaacccat ctgtcaactg atgtcctccc ccagcaagct
      961 tattagaacc atctgcaatt ctatcagaac cactagctaa ttgccccgct ccgtctctca
     1021 attgatccga tttactatct aaagtttgag ctccctgact tgctttttgt gttcctgaaa
     1081 tcaattgatt ggatttacta tctaaagtta ctgcaccttt gcgtaattgc cctacagcat
     1141 tcgtataatc cgtgattcct tttgtcaagg tttgactacc ttgatataac tgcttagtag
     1201 ccgttggcaa tttttgaatt ttatctaaat ctgagatagc ttttaatact aaattgttag
     1261 ctgttcctga caaagtttga agattttgtg atccattatt agaaggggct gaactagatg
     1321 cttcactaag cacagttttc atattttcta tatttgaaga aatatttgct gcaattgtct
     1381 cagctgaagt aggtgttcca gtaaccgctg aggtgatttg agattgttgt tcaggggtta
     1441 aacttttaaa agctgacgtt gattgtaccg cagtcatttg cttttgttta tcagcagcag
     1501 ccgtattcat caaagccttt gtacttgatt caatagttga taaaaaatta aataacttac
     1561 tattagtatc gctatcagta gttgctgtcg attgtaaatt tgtaataatc tgatttagat
     1621 tctttaatcc atctgaaagg ttctgcaaag tacgttcttg ctctggtttt agttgagttg
     1681 cttgtgacaa ttgttgtaaa ccatccgtca acttttctga tccttccacc aatgcttgag
     1741 acttatcatc taatttttga ctaccttcac tcaacttatg cacacctgtt gtgtaagcag
     1801 taacaccatc tgataatgtt tctaacccgt tagatagggt ccccacacca tctgtataaa
     1861 ggtttattcc actatttagt ttattagctc ctccagaaaa agtttgacta gagcttgcta
     1921 aaacatctaa attacttgtc aaggtttggc tcccatcctg tagcttatta gaaccattta
     1981 ataattcttg actcccatca gcagctttat tgatacctgt tttcatagaa cccatatttt
     2041 taaaaactga ttcagtataa gtccctgtaa tagattctgc tacctcatct tttagtttat
     2101 tagctgctgt ctcactcatt tttgaagcta caaaactacg ccccttagta gttttataag
     2161 taatatttaa cctttctggt ttttctgtca tgagagttgt cgcttttcta gaaagatctt
     2221 taggaaaggt aataaccata taatagtgac ccttttctaa ttctttttct gctcttttag
     2281 ctgttgtaaa atggaaatct aagctatcat ttttagacaa attgtcctcc atatctttgc
     2341 caattgaaat agtttttcca tttaatttag ccgatttatc ttggttaacg actgccacag
     2401 gtaaattttt agtattccca taaggatccc acattgagct tagaaagata acattatata
     2461 gcgttggaat cagtgcaacc ccagccatcg taatccataa ttttgggctt tttatgattg
     2521 cttttaattc gtctaacatt ttttctccta ttttggacat attgttcatt tttttgataa
     2581 aatatattat acagtgatga tagataattt caagataaaa atctcgtttt tggacactgt
     2641 gtttaaaaat gtacaaaaat attaaggggt aattaatatg gtaaacgata cgaggagaga
     2701 aaaaacaaaa agagctattg aagcagctat gattacgtta ttaaaagatc agagcttcga
     2761 tgaaatttca acgattaatc taactaaaac agctggaatc agtcgttcta gtttttatac
     2821 tcattataaa gataaatatg aaatgattga tcaataccaa cagtcactct tcaataaagt
     2881 cgaatatatt tttgatcgca atcaatttaa aaaagaagat gccttgctcg aaatttttca
     2941 attcttagat agagaatcat tatttgctgc tctcttaacc caaaatggaa caaaagaaat
     3001 tcagacctac atccttaata aattacaatt aatgctttct aaagaattac ctgtggtcaa
     3061 tccagatgca acaaagtctg atatcaaccg tttgtattat agtgtctatc tcagtcatgc
     3121 tatttttgga gtctatcaaa tgtggataac acgaggaaaa aaagaaagcc ctcaacaaat
     3181 tacacaagtg ctactcagtt tattacctca aacttaaaac cagccttcct tataggctgg
     3241 tcatttttag ttaggtttat tttatcctca ttataggata acaacactaa taaggactga
     3301 cattataaat gacgatacat ttgagaagac atatctaaaa attggcctac aagctcgtta
     3361 tgttctccta taaaatcaaa gagctgatcc tcactcagta caccgcattt gagattattt
     3421 tctgtttgtt ctgatagtct aaaccagtct tcgctaccat aaaattctct taatttcacg
     3481 taatcttcat agtgcttttg aaaatcatct atagcttcta ctaatgcatt gattctaggt
     3541 ttgaagctat catacttctc ttccatttct tctattttta atctatcatt tagattcata
     3601 ataaaatctc cttagttata taagattcga cgagataagt tatcattctc aagtatttgt
     3661 aacgttaact ctactatatc ctttttacaa agtaaaaaat tactcattac aaactgacag
     3721 tgttgatttt tattctaatg ttagtttaaa atctttttgg ttcattaaat ataatgactc
     3781 actaaaaagt atatagcaaa aaaagcctat aaaataggct tttaaagtat gtaatgtaat
     3841 aattaaagca ttttgttgta gaattcaacg acaagtgctt cattgatttc tgggttaatt
     3901 tcatcgcgtt ctggaaggcg agtcaatgaa ccttcaagtt tttcagcatc aaatgatacg
     3961 aaagctggac gtcctaaagt agcttcaaca gcttcaagga ttgcaggtac tttcattgat
     4021 ttttcacgaa ctgaaataac ttgtcctgga gtaacgcggt atgatgggat atcaacacgt
     4081 ttaccatcaa caaggatatg accatggtta acaaattgac gagcttggcg acgagttgtt
     4141 gcaagtccta aacgataaac aacgttatca agacgacgct ctaaaagaac cataaagttg
     4201 aaaccaagag ttccttcttt tgctttagta gcttgtacga acaagttacg gaattgtttt
     4261 tcaccaagtc cgtatgaaaa acgaagtttt tgtttctcag ctaactgcaa gccatattct
     4321 gaaagcttgc tacggttatt aggaccgtgc tgaccaggta cgtagttacg acgtgccaat
     4381 tctttacctg tgcctgtaag tgataaacca aggcgacgtg attgtttcca tgatggacca
     4441 gtataacgtg acatctaaat gtcctcctct aaaataattg aggaaataac gatttaaaaa
     4501 atcctgattc gtgcagggga ttttcgccta aacagcaaag ggtactagat tagttaacca
     4561 cctgttgacg agcttcatat ttttctgctg ttatttcaca caagctatat tatatcatga
     4621 aaataatatt gaatcaagtt tttatgtctc ttaaacctta aatacaaata gacatctaat
     4681 aagtatttcc aattataata agaaagattc ttaattctaa ccaccaatag agaattagta
     4741 aattttttta tagaaacaaa aaacctcagt cgaggttttc atactattct gctgactcgt
     4801 catcaaaata acgaattaaa gttttctcag ttaaaatatc tgaataagtg taagactcta
     4861 cataagtatt atcaattgct cctggtacag ctgcagtatc cttatactct acgataaaca
     4921 aagatgggta aacttcaatt aggcgcccaa ttttattttt ttccctctta cgaccatttt
     4981 cgagagtaag ttcgaccatc tgtccctcat gtgacttgat atcttctttt atttttttca
     5041 tttttgcaac atctgcaaat gcatcactca tattaacatc tcctattctt caaattgagc
     5101 tatactagaa aatttattat attccttttg aaacattaat tttactgtcc cacgcgcacc
     5161 agcacggttc ttttctaaaa taacctcaac agtattatct tctacaattt cctctgcttc
     5221 ttcgccctct ctacgataat aatcatctcg atataagaaa gcaacaatgt cagcatcttg
     5281 ttcaatcgat ccagattcac gaatatcaga taagaccggt cgtttatcct gcctttgctc
     5341 aaccccacga gagagctggc ttaatgcgat aacaggaacc tttaattctt tagctaaaat
     5401 cttcaactgc cttgaaattt cagaaacttc ttgctgacga ttttctggtc tagttcctga
     5461 aattaattgg aggtagtcaa tgacgattaa acctaaacca tcatcaactt cttgagataa
     5521 cttcctagag cgtgctctaa tttctgtaat tttaatcccg ggcgtatcat caatataaat
     5581 tggcgcatcc gccaaagctc cttgagcaat cgttacatta ttccaatctt gatccgtcaa
     5641 ctgaccagtt cgtaaactat gagaatcaac cattccctct gctgccaaca tacggtcaac
     5701 taaactctca gcccccattt caagtgaaaa aatagctaca ggtctatttt gtttagttcc
     5761 aacattttga gcaatattta aaacaaaggc tgttttacct actgcaggac gggccgctaa
     5821 gatgattaat tgatctggat gtaaacctgt agtaatcctg tctaaatctc taaatccagt
     5881 tggtaaacct gtgacatctg aggtttgctg tgaacgtaat tctaagtttt cgtaattaac
     5941 ttttaaaaca tcagatatct ttctaaaccc gctacgatta ctgtgttcgt taatatcaac
     6001 taaagccttc tcagcggtgg caataatttc atcagaatca tttccttcat aggccatgtt
     6061 gacagtatct gtcaacttag aaatgatatc tcgcaacatg gctttctcag aaactatttt
     6121 ggcataaaac tccgcattag cacttgttgg aacactatta actaattcaa caatatagcc
     6181 caacccacca atgttttgaa gatcaccttg gtcatctaaa atatttctaa cagtagcagc
     6241 atcaattgca tcatttctat cagctaaggt aatcatcgct ctgaagataa ctttgtgtga
     6301 atatttataa aaatcatctg gacttataaa ttctctcacc ataatcagtt tctctggtga
     6361 gataaagata gatcctagaa cggcctgttc tgccaacagg tcttgaggtt gtactcttag
     6421 ctcagatact tctaccaaac ttcattcctc ctgacttgtc aacctttatc atctctcagt
     6481 ttacgcttct ttgatatcaa gcttaatctg tgaagaaacc tgtttatgta attttactgg
     6541 tacttcaacc ttaccaatcg ctctaatagt atgatctaaa tcaatatgac gtttgtcaat
     6601 tttaatgccg tattgttttt gtaactcttc agcaatcttt ttggctgtga tagaaccaaa
     6661 cgtacgacca tctggaccta ccttttcaat aaattgcact cgggtagttt cggattctaa
     6721 ttgtgttttc agctcctttg cctgtgctaa aatttctgct tgtgcttttt cttcggactt
     6781 ttgttttcct tttaattcac cgatagcttg tgtcgttgct tctttagcta gattcttttt
     6841 taataagaag ttttgagcat aaccagtagg tacttcctta acctcacctt tttttccttt
     6901 ccccttaaca tcttgtaaaa aaataacttt catctcaatt aatcccttct attttcgact
     6961 gtactatttt ctcttaaatc ttcgtcaatt atttccagta acatacgtct aacttgcttt
     7021 acagacttgt cttgtatttg acaagcagca aaactaaaat gtccgccacc accaagtttt
     7081 tccataatac gctggacatt gatattattt ctactgcgcg ctgaaatacc aatatttgaa
     7141 ctgtttttag taataacaaa agtagcctca atacctgcca ttgtcaacat ggtgtcagct
     7201 gctttactag ctataacatg cgagtaaact ttatgttctt ctccggtagc aacaatgatg
     7261 ttatcataaa tacgctcacc ttttaaaatc aattcattaa ttaaacgata ttcatcaaaa
     7321 tctgtagctg atattttttg aatagccata ctattactac ctaaaccgcg taaatagctt
     7381 gccacatcaa aagttctact cgttacatta gatgcaaaat ttcttgtatc aagcatgata
     7441 ccagccatta aaatacttgc ttgactgcgg cttaatttat ctttagcttg ttgaaattga
     7501 atcaattctg taactaattc gctggcacta cttgcgccac tttctataaa tgaaagaaca
     7561 gcattttttg gaaaatcttc atctcttcta tgatgatcta ccacaataac atctgcaaac
     7621 ttgttaaaga attccttaga taaggttaaa gctgttttag aatggtctac catgactaat
     7681 aaagaatttt gagtaattaa ttcaaaagct ctctcaacac ttacgagacg tgtttcgccg
     7741 tcttcttgca agtaatcaat tgctctttca atatcagaat tcatgtcatt tgggtcataa
     7801 actacataac tggcattaac aatattacta gcaaaaaatt gcatccctac agatgcacct
     7861 agtgcatcca tgtccaattt cctgtgtccc acaataaaga cactatcaac tactttcaaa
     7921 cggtcagaaa tagcagtcat catagctctg gtacgtgttc gtgaacgttt aatagttgaa
     7981 acagcaccac caccaaagta aagtgctttt ttactactat cattttcacg tactactatt
     8041 tgatctcctc cgcgaacgag tgctgtatta agattttcta aagcaatttg cccaatttga
     8101 ttatggttac catcaccata agaaattccc atacttaatg ttaatgataa atggttttcc
     8161 tgagcgcgtt ttcgaaactc atttaaaata tcaaatttat cttttatcaa tgtatttaaa
     8221 acgctatagt cggtaaaaat atagtatcga tccatattga cacgacgata aaaaatattt
     8281 ttagactgtg taaaatcaga aataaaacta gtcacgaagg cgttaatctt tgacatatca
     8341 gcctctaaca tagtatccat aatatcatcg tagttatcaa tggaaataat accaataact
     8401 ggacgtagca ttgaactatc atagtttctt ctattcccca tgaaattatc gaagaaatag
     8461 aaaacacccg tctctgtatc caaataagct gaatatttgt tatcaccgta ttcaaatgat
     8521 tgcccagccg ttccattacg tctactagta atgatatctt taacagtagc agactgaaaa
     8581 tgaccattat ccccagtaaa aatcaattct gcataagggt taaaccactc aatatcattt
     8641 gtttcaagat caaattgtat aacaccaact ggcatttgtt caagaagact cttaagagaa
     8701 acttctgttt gatcattaag taattcaatt tgttcaactt cagatagttc ataagttatc
     8761 ttttggtaat ataataaggc aacaacaaaa gataaagcaa caaaaatagc caataataga
     8821 gccgtatagg attgaaataa cctaacacaa atagctaata gtccaaataa aatcaggcct
     8881 attaaaacta aatggacggt tgcaaaacga aatcttttca ttaaataacc tcttaagacc
     8941 taattataac acaaaacgcc cctgataaac aggggcgaac actattatca attgatattt
     9001 ttacgacctt tttgtcttcc ttcaaggtaa accattaaaa tactaatatc ggctggatta
     9061 actcctgaaa tacgactagc ctgacctagc gtttctgggt taattttttt gaatttttgt
     9121 ctagcttctg ttgcgataga atcaatgtcg tcccagtcaa tatgaggggg aatacgtttt
     9181 tcttccattc gtttcatttt tgcaacttga tctaaagcct ttttaatata gccttcgtat
     9241 ttaatttcag tttccaaaag ttcaataacc ttactgtcta atggctcatc agcacaacca
     9301 acaaaatccg ttgctactgc atatgtgatt tgtgggcggc gcataaattc tttggcagtt
     9361 aaagcatctg tcaaaggttt aaagcccaat tcttgaatcc gtttatttgt ctcagaaact
     9421 ggctttaact ttatagagtc taaacgttct aattcatttt caaattgcat ttgacgcttt
     9481 ttaaatatcg cataacgctc ttcatctact aggccaatct cataaccgat ttctgttaaa
     9541 cgcatatctg cattatcatg acgaagaatc agacgatatt ctgcgcgact tgtcaacaga
     9601 cgatatggtt ctagagtccc tttggtaact aaatcatcaa tcatgacacc aatgtaagca
     9661 tcacttcgtt ttaaaataag ttctggcttc ccttgaactt taagagcagc gttgatccca
     9721 gcaaccaacc cttgaccagc agcttcttca taacctgaag taccattagt ctgtcctgca
     9781 gtaaacaaac cagcaattac ttttgtttcg agtgttgctc ttaattgatg aggcaataca
     9841 atatcatact caatagcata gcctgtacgc atcatttcag cattttcaag cccttttata
     9901 gaacgtaata aatcaacttg aacatcctca ggaagactcg ttgatagccc ttgaacataa
     9961 acttcttcag tgtatctacc ttctggttct aagaagagtt ggtggcgctc tttatcagca
    10021 aaacgcacaa ttttatcttc aattgacgga caatatctag gaccaactcc cttaacgatt
    10081 ccagaaaaca taggagcacg gtgtagattt tgattgatga tgtcatgact caaagtattc
    10141 gtataggtca accagcacgg tacctggtca gtaatataat cttcatccct agacataaat
    10201 gaaaaatgat ttggttgctc atccccaggt tgtatctctg tcttttcata attaatagag
    10261 gaggctttta cacgaggagg tgttccagtt ttgaaacgac caatttctaa tcctaggtct
    10321 cttaaattat ctgccaatgt cacagaagct aaactattat tagggcccga ggagtactta
    10381 agatcaccta atataatctc tcctcgaaga gcagtacctg ttgttataac aacagatttt
    10441 gctgaaaatt tctgattagt tgctgtacga acccccacaa cctttccatc ttcaacaagt
    10501 atttcgtcaa tcatagcttg tcttaaggta agattttctt gcttttctac agtttgcttc
    10561 attgtttgtg catagagggc tttatcagct tgtgctctta aggcacggac agcaggcccc
    10621 ttacctgtat taagcatttt catttgtata taggttttat caatgttttt acccatttca
    10681 ccacctaaag cgtcaatctc tctgacaacg atacctttag cagagccacc tatagaagga
    10741 ttacaaggca taaatgctaa catttctaag ttgatagtgg ctagaagtgt cttacatccc
    10801 atacgactag cagctagact agcttccaca cctgcatgac ccgctccaac aacgataata
    10861 tcatagtttt ctgcaaaatt atgtgtcatt ttattccttt ccaatttcaa agtctaattc
    10921 attaaaacac aaaaatagcc aaaactctcg aaaattgtac gtcaaaaaga cttacaaatt
    10981 ccatgagctt tgaccatcat agttattgtt aaatttattt tagcatttat cttttaaaag
    11041 tcaaccagag gggataggct atgttaaatg aaaaccaatt tttatgcctg ttattagcat
    11101 ctctatcccg atagtcatca aaataagtcc catcattttg gtgattatag tcattgcatt
    11161 ttttccaaga aatttagtaa tttcgtttgc acttattaaa ataacataca ttatcacaca
    11221 taatatggca aaagcaagta tagtaatgag tttgccagat aaaccaccac ttgataaccc
    11281 catagcagtt gcaatagtac ccggacctgc caaaaggggc attgctaatg gtgaaactgc
    11341 cacattcatt ggatcatcct tatgctcctc taggttttta ttggttggtg aatgtatacc
    11401 attaatcatt ttataaccta taatgcctac caatattcca ccagatattt ttagggcggc
    11461 tagggtaatc ccaaataagt taaaaagtaa gtggccactt aatacaaaga ttacaataat
    11521 gacaaaagca agtaatactc cttttgctgc aatccgacgt gaaatcttct gatcatcatc
    11581 agctactaaa gccataaaag ctggcaaatt agaaatggga ttcataatgg cgaaaaaagc
    11641 catgaaagct aaaataaatt ttgaaatcat acgattacct cacttaataa ttaagtgtca
    11701 attaaatgta ttgacagatt ttatcatcac gataagcctg atcaatcatc ccaccgccta
    11761 aacattcttg ttcattataa aaaacaacag cttgacctgg tgtgattgct cgttgtaaat
    11821 catcaaacac aacccttgct tgattacctt ttacgtaaac tgtaacttta ctgtcaggtt
    11881 gacgatatct gaacttagct gtacattcta gcttaaattc attaggcata tcacgtgtaa
    11941 agtggatttc agaagctgtc aagctagttg acatcaatga gtcatgatag aaaccttgac
    12001 ctacataaag aatgttttta cttaaatcct ttccaactac aaaccaaggt ttattatctc
    12061 caccatgttg cccaccaata ccaaggcccc ctctttgacc aatagtataa tacattaaac
    12121 cagcatgttc acccatgtca cgaccatcaa cagtcatcat acgacctggt tgagccggta
    12181 aatattgacc caggaaatcc ttgaaatttt tttctccaat aaaacaaata ccagtagagt
    12241 cttttttctt agcggtcgca agtcctgctt cttcagctat acggcgaact tctggttttt
    12301 gtaaatgacc aagtggaaat aacgtttttt gcaattgctc ctgagataat tgacttagaa
    12361 aataagtttg atccttatta ttatctgctc ctctaagcat atgaactatt ccgttctcat
    12421 cacgtgttac ttgtgcataa tgacctgttg ctacatagtc agcacctaat gtcatcgcat
    12481 aatctagaaa tgctttaaat ttaatttcct tattacacat aacatctgga tttggcgtac
    12541 gacctgctcg atattctgct aaaaaatatt cgaaaaccct gtcccaatac tctttttcaa
    12601 agttgacaga gtagtatggt atgccgatct gatcagcaac agcagcaaca tctttataat
    12661 cttcagtagc cgtacaaaca ccaaattcat ctgtgtcatc ccaatttttc ataaaaacac
    12721 caataacatc ataaccttgc tcttttaaaa gcagggcagt tactgatgaa tcaacacctc
    12781 cactcatgcc tacaacgaca cgaatgtttg aattatcagt cataagaatt ctcccatcaa
    12841 aaagttttat ttttaactta ggcttgaagg gccaagttgt tcttagatca atcatattga
    12901 ctaagaaaca ctataaaagt gcttatatat tatagcacta tttgttgtca agttgtatgt
    12961 ttttgttgtg ataaatattt attacattgc agttttttta tctttttatg atatttttta
    13021 gaaataatct gttataatga gaatgtacaa aaatcacgag gaaaaactat gaaacattta
    13081 aaatttcaat cggtcttcga cattattggt cctgttatga ttggaccatc aagtagtcat
    13141 actgcaggag ctgtccgcat tggtaaagtt gtccattcta tttttggtga acctagtgaa
    13201 gtaacctttc atttatacaa ttcttttgct aaaacttacc aaggacacgg tactgataaa
    13261 gcattggttg cagggattct aggaatggat acagataatc cagatattaa aaactctctt
    13321 gaaattgctc atcaaaaagg aattaagatt tactgggata tcctaaaaga tagtaattca
    13381 ccacatccaa atactgcgaa aataactgtt aaaaatgggg ataggagcat gagtattaca
    13441 ggagtgtcaa ttggtggcgg aaacatccaa gttactgagt tgaatggatt ctcagtttca
    13501 ttaacaatga atactcctac tttaattatc gttcatcaag atatacctgg catgattgct
    13561 aaagtaactg atattttatc tgactttaac attaatattg ctcaaatgaa tgtaacacgc
    13621 gaaagtgcag gagaaaaagc tatcatgatt atagaagtag attcacgaga ttgtcaacaa
    13681 gctgtcaaaa agatagaagc tattcctcat ttacacaatg tcaacttctt tgattaagag
    13741 aaagggtaac tatgttttat acaattgaag agctggtaga gcaagctaat agccaacata
    13801 agggtaacat agcagagctc atgatccaaa cggaaattga aatgactggt agaagtcgtg
    13861 aagaaattcg ttatattatg tcccgaaatc ttgaagtcat gaaagcttct gttattgatg
    13921 gattaacccc tagtaaatca atcagtggtt taacaggcgg tgatgctgtc aagatggatc
    13981 aatatttaca atcaggaaaa actatttcag ataccacaat cctagctgcc gttaggaatg
    14041 ctatggctgt taatgagtta aatgctaaga tgggactggt ctgtgcaaca ccaactgcag
    14101 gtagtgcagg atgtttacca gctgtgattt ctacagccat tgaaaagctt aatttaacag
    14161 aagaagagca acttgatttt ctatttacag ccggcgcatt tggtctcgtc attggtaata
    14221 atgcctctat ctcaggtgca gaaggaggtt gccaagctga agttgggtca gctagtgcta
    14281 tggctgcggc tgctttagtt atggctgctg gaggtactcc tttccaagct agccaagcta
    14341 tagcatttgt tattaaaaat atgcttggac ttatctgtga ccctgttgca ggtttagttg
    14401 aagtcccttg tgtgaagcgg aatgctcttg gatcaagttt tgcacttgtt gctgctgata
    14461 tggccttggc tggtattgaa tcgcaaattc cagtagatga agttattgat gcaatgtatc
    14521 aagttggatc aagtttaccg actgcttttc gtgagactgc agaaggagga cttgctgcca
    14581 cgccgacagg aagacgttat agtaaagaaa tttttgggga ataaagttta tcaattttct
    14641 actctttccc tgaaaataaa taaaaacgct acagctttac tgtagcgttt ttatttattt
    14701 gttttagcaa tctgtaatta ttgttgacaa cagtgtaaag ttaatttata gctattcact
    14761 agtaacccca agctgataaa ccttgagcac gataagcttt aatagctgaa ttaacttgat
    14821 cctgaactgt agctgttgaa ccccaacctg gcatcgtttg gaaaagtcct gaagctcctg
    14881 aggcattagc aacattagga ttaccatttg attcacgggc aataatatgt tcccaagtag
    14941 actgagggac tcctgttgca gcagccattt gtgctgcagc tgctgagcca atagcccctg
    15001 cagtatttcc attactcaat acttggccac tcgtctggtg ttgagcaggt ctataagttg
    15061 tctcagtaac agcataagct tgttgtgcct gactggtagc aggggtgttt tctgttacaa
    15121 ctgcttgttc tacagctgcc tcttcactcg cagtaacttg ttgctgagaa ttagcttctg
    15181 tagattgaga acttgatttt ggggcttcat tagatgcctg agaaggtttt ggagcctgtt
    15241 ttacatcttc tacttttgat ttagatgtcg ccttagtcat ttttgatttt ttggctacgc
    15301 gaactttatc tgcttttgac aaagattcag tagtaacttg tgaactttta aagatgacca
    15361 ttacagccaa aatacatgaa aaaagggcta ctaaagtgat agctccataa gctaaatgat
    15421 gtttttttac attcaatttt gataattttc ttcttttatt catgttacct atattatact
    15481 ttatttgtta ccttgcaatt gaatagatgt taaaaatatt acaaagagat ttaattttaa
    15541 gtaaatctct ttgttttatc aacttttatt aataccagcc gttactattc caaaatgata
    15601 gcgctgccga ccaagatccg taacgagaaa ccacataatt gtccgctact ttttcttgat
    15661 tttcaggaga taagtcgcca tttaggtaag attgagacag ttgatatctt ccataatatt
    15721 gtccattctg tgcagtataa ctaccatttg attcacgacg agctatttct tcttttgcgg
    15781 ctgaatcact tgaactcaaa tttgatgaag aattcattga attttcaatt gttggttgag
    15841 attttgcttc tgcttgacta gctgtagaat tatccaattt taaaacatca cctatactga
    15901 tgacatcagc gttactgata ctattgagag acactaactc ttgtaccgta gttttatgat
    15961 ttttagcaat agctgataag gtatcacctg atttcacggt ataagatgct gcgcttactg
    16021 aaagtggtaa agctaatgaa gcacccacta aaagacctgc cactcctaat ttaaatggtt
    16081 ttgatttttt aaccgaggtt attgacataa tatatgctct ctttcattaa ttgatacgcg
    16141 tactatcata ccttgtaaat attactaatt gttttgcctt agatgaaatt tattacaaat
    16201 aaattgtaaa ttaataaaga aaaggcctcc aaaaaacaat ttttagtttt ctttaagtaa
    16261 agttgttgag cttttcacat tatataaatg aaaaggctaa gaccataaac tgatcttaac
    16321 cttttaattt tttaatagaa acaaactcaa acccaataat aacataagta atatactaaa
    16381 agtatcccga acagtccact tcaataaacg gtacttacta cgattggctc cgccttgata
    16441 acctcttgct tccatagcaa ttgccaacgc atctgctctt ttaaaactag aagcaaataa
    16501 tggaatcaaa atagggataa tagacttaac cttatgaatt aggtttccct ctccaaaatc
    16561 aactccacga gctctttgag cattcatgat acgtgttgta tcatccatta aagtaggaac
    16621 gaaacgtaag ctaagggata acatcaaacc aatctcgtgt gctggtactc tcagtacttc
    16681 caatggtttt aacaaagatt caacagcatc tgctaaactt aacggagtag tagttaaggt
    16741 aagtaatgtc gaaaagaaga taattaaaac aaatctcata aaaataagtg ctgcttgttg
    16801 taacccaagg ctagtgatgc taaaaatcca aaacgagaag ataacctgcc cgccttgtgc
    16861 aaacagcatc tggaagaggg ttgtaaataa aataatacca accatcggtt taataccatt
    16921 gaaaaaatag gaaaatttta tctgagataa acccacaata accaacgtaa aaatgaatac
    16981 gatcacattt gtcactacat tatttgccca aaaaacaata atgataaaaa gcaacatggc
    17041 tagtaactta cttcgaggat ctaatttatg aatgagagaa ttacctggta tgtaacgtcc
    17101 caaaatcaat ttatccatgc ttaatagcct ccacaaattc gttaatagta attggtaaac
    17161 taggtaaatt taatccctta tgagatagtc tttgagcaaa cttggtgatt ttgggaactc
    17221 ctaattgttt actttctaaa agttctactt cttgaaaaat ctgttttggt tgtcctgata
    17281 aggttacttt ccctgcttct aaaacataca catagtcagc ataatccgct acatcgtcca
    17341 ttaagtgagt cactaagacg atagtcattc cttttttatg aagattttta aaaagagtca
    17401 ttaattcttt tcttccctta ggatcaagtc cagctgttgg ctcatccagt actagtactt
    17461 tgggttccat cgctaaaata ccagctatag caacccgcct catctgccct ccagaaagtt
    17521 caaatggatt tttatcgaat aaatcctcac tgataccaac taaccttaat ttttcttcag
    17581 ccagcctttc agcttcaatc tgagaaatac caaaattttg tggtccaaaa gcaacatcct
    17641 ttaaaactgt ctcttcaaaa agctgacttt ctggaaattg aaaaactaaa ccaacttttt
    17701 gccttataaa tttgatttct ttgttcttgt cccctgcttt aatagaaaaa tcatcgacaa
    17761 ttacctcacc ttttgtagga atatgtaaac cattcaaaag ttgcataata gttgattttc
    17821 cagaacctgt gtgcccaatg aacgcggtat aggaagcatc ttcaattttc agattgacgt
    17881 caaaaagggc acgcccttca aaaggagtgc cggcttgata ggtataactt acatttttaa
    17941 attcaattcc cataattgtt cttctaattc cttctctgta agatactctg tccctatatc
    18001 aagaccatta gcagccagtt ctgccattaa cctacttgta aagggaacat ctaaacctaa
    18061 actaatcaaa cgatttccac gaccaaataa cgcttttgga gtggatgtcg actcaacttt
    18121 accatttttc ataacgatga cacgatcact caatgcaact tcatctaagt cgtgtgtaat
    18181 tgaaataaca gtaagattat atttttgacg aatagctcgt atcgttctta tcagttccaa
    18241 tcgtccctca ggatcaagca tactagtcgc ctcatccaag atgataactt gtggacgcat
    18301 agctacagct cctgcaatgg ctacacgctg cttttgtcct ccagataaac gtgcaggctc
    18361 cctcatttta aattcagaca ttcctaccaa atcaagtgct tggtccactc tttctttcat
    18421 gtctttcaaa ggaatacctt tattttccaa accaaaggcg acgtcatctt caacagttgc
    18481 ccctacaaat tggttatcag gattttgaaa taccatgcca atcttgtgac gtaactccca
    18541 gacattatct tcggttaact cttgtccatc aatgataatc tggccagact ccgcctctaa
    18601 aagtccatct attaaacgaa cagtagtgga tttcccagaa ccattatgac caataataga
    18661 tagccattct ccttgtttca catgaaacga tacattctcc aattgataat gtgtttgatt
    18721 actatcatat ttaaaaaaga gattattaac ggtaataata tttgtcatta tttaaaagta
    18781 tccttaaata agaagcctgc acctttaaaa tagtcgtagc ctgaatacaa ggtaaagaat
    18841 agagcaatgt ataacatgat gttaccaagt gtcatccaat gtactaacaa aaaaataact
    18901 gcaaacattt gagtagccgt ctttattttc cctggcatag ctgcagcaag tactgttcca
    18961 cctgtttcaa ctaaaagtaa acgcaaacct gtaactgcta actctctaca aatgataata
    19021 gctgaaaccc aagctggtgc taagtctagt ccaactaaca tgataaaagc actcataact
    19081 agcattttat cagctaatgg gtctgcaaat ttcccaaaat ttgttactac ttgccattta
    19141 cgtgctagat aaccatctag gtaatcggtc aaacttgcaa tagcaaatac aatagctgca
    19201 acaatatgcc aagttgtact tgttgttact gaagttaata ctataaataa cggaatcatt
    19261 aaaattctga ctacagttaa tagattcgga atattttctt tcttcatcat aaattctcct
    19321 tttggaaaca tagtattcct atattatgtt atttcaaaac tagtaaaaga cttttgacat
    19381 accaacttta tagatgtcta agcttcctta ttatatcaaa atttcagatt atttacgaag
    19441 attttggaaa aataacgaag tcagatgtgt cagcattatc taagaaatcc ttaccaatcg
    19501 taatgacatc ttccaaatct aattcttcaa cgatagatgg caaatccaaa tacatcgttt
    19561 ttccatcata taaataggtg acgaattgca ttgcgagatt ttcaatagaa tctaagcttc
    19621 ttaaaaaatc gccatagagc tctctcttaa cgttttttaa gtggctttcg gtaagatcag
    19681 atgattgcaa agcatttttt agcaataacc tcaactgagt tgaaaaagca attggctctg
    19741 tcgtatctaa agaaataata acgcactcaa aatctggatg aacttcaatt tctatatcaa
    19801 aagaatcatc aatttgacca gattcgtacc agtcttggtt aattgtagaa gtccatccta
    19861 aaagcattgc aaaaaatagc tgtaatccga ttttctcttt taggtaagaa ccatgtatca
    19921 tatgatttga ttttcgataa ccaatagcta atttaggttt tgtcactgtc atatggcaag
    19981 agttgttttt tataacaggc tcaaggctaa tagaatctcg gtcaatattc tgtgagtaac
    20041 ttgtgaaatg agaatttttt tgaaggtaag ttataatttc ttgtggacta aattgtccaa
    20101 caagaactaa attcatatta gacaactgat aaaagtcttt gtgattttct tgtaaatcgg
    20161 ttaaggttat ttgagagatt gattggtaat ctccagcgat atcaaatgct aatggagagt
    20221 tggggtaaag gttcgataac actccttgat aaagcctata ttctgggtca tcttgataca
    20281 tttcaatttc ttgtttgata atatcttttt ctttagtaat tgactcttct gtaaagtttg
    20341 ttgtcaatac aaagtcaagt agtatatcta agcagtttgt aatgtgactt atagttgaaa
    20401 aataaaaact agttttatca aaagttgtaa aggcgttact ctcggcgcca tatttagtaa
    20461 attgagttgc agcgtcttgt cctttatcca gttcaaataa tttatgttct aagaagtgag
    20521 caattcctgc cggataatgt tctacacaac cattcctcgt atattttgta tggagtgatc
    20581 caaaatttgc tgtcaatact ccaacagttt ccttaaatga tggctttgga ataaggtata
    20641 catttagtcc gctctctaat gttaacttat atacttcctc ctgaaggttc tgataagtta
    20701 ttttattaac tgtcatccta gtttccttcc aaaaaataaa aagcttgtaa ttttaatttt
    20761 gttgctacgc tgacaatatc tgattttgtt acatcgtcta ctttgtcaat gaatagtgct
    20821 gaagtatgaa actgatctaa ataatgttca tgataaatgt ggtcaactat gaccttagga
    20881 ttatcagaag ctaaaacata gttcattttc aatatatctt ttgtttgttt caataatgta
    20941 gaagaaaacc tgcccatctt aatattattg aattgcttat taataagctt caaaaaacgc
    21001 tcacgattct ctttatcaat acctgcatat atcgtaaaga gacctgtaaa gctgtcaaat
    21061 tgactaccta tagtataggc taaaccttgc ttttctcgaa tttctgtaaa caacaaggag
    21121 tgggcaaaag caccaaataa accgttaaat actattaaag caaaataatc ttcatcttta
    21181 taagttattg gaagatgata agccaattgc ataatagatt gattaacgtc cttatcttct
    21241 acttgacttc tcgttatatt ttcataagtc tgcgtataat caaaagctaa tactttgtga
    21301 cgtggctcaa atgccatacg attaaaagcc tgaatcattc gatagtcatc aaaatcccct
    21361 acgacaaaaa tatctaattg atcttcctta agcatcttct gaaattcttg ataagctgta
    21421 aatgaatttt ctgattccac taaagatgct gtaccatact taggtaaacg aagtgtctta
    21481 ttattgtaga ataaagattt tagtgctaag tcactgctat aaaaattatc ttcaatatca
    21541 gcctctaaat attgaataag gtttttcttc tcagtatcaa aaatagatgt ttgatactgt
    21601 tctaaagaaa ttaaaggaga aaacagcata tcctccaaga atgtaataat ttgttcaacg
    21661 atattttctt gttctaaagt aaaggtattt ttaacaaata caatatcaat atcaacaatg
    21721 tgaacaagac ccttagtaga aatcttagtt gataaactag cgccatataa ggaagctaac
    21781 ttttctcgaa attcttgtac cttaggatac ttagcatttg cagtaactaa catttgagca
    21841 acgagcgatc tacgtgctac tgttttatta ttaaaatcac ctgaaaatcg aaaagtaaga
    21901 tgattagtct taaattgttg gtttttaatt aaatgtagag aaacgccttc aactattttc
    21961 attcaatatc ctttgtgttc tgtattgtta gctaagatta tatcattttt aaaggcgtta
    22021 aaaaagatta aacatccaag atatgattaa ttctaaattt caatcttctt caaaattgac
    22081 aaagtcgtca accaatattt actcccattt tttatacctt gtctccatat cataagttca
    22141 actcaaaaaa taattttatg ctataatgat gtgatatcta aaattggaga ttaaaaagtc
    22201 atggattata aattatttga tgaatatatt actttacaat cacttctaaa agagattggt
    22261 atcattcaaa gtggcggagc cattaaaaaa tttttagcgg ataatcgagt attgtttaac
    22321 ggtgaccttg aaaataggcg tggaaagaaa ctccgcctgg gggatattat tactattcct
    22381 gatcaaaata tcgagatcat tataaggaag ccatcagatc aagaaattga agaacgtaat
    22441 atagaaatag ctgaaaaaca gcgtgtttca gcaatcgtta aagaaatgaa taaaaacacc
    22501 aacaaaggta aatcaaaaac tagcaaaaaa ccagttcgct ttccagggac ataaaaatgt
    22561 ggataaaaaa tatttcttta aaacattatc gtaattatga agaagcacaa gtagattttt
    22621 cacccaacct caatattttc attggccgca atgctcaagg aaagacaaat tttttagaag
    22681 caatctactt cttggctttg actcgaagtc accgtacccg ttctgataaa gaacttgtac
    22741 attttaaaca ccatgatgta caaattacag gtgaggtaat ccgcaaaagt ggccatctta
    22801 acttagatat tcaattatct gaaaaaggaa gaatcactaa agttaaccat cttaaacagg
    22861 caaaattatc tgattatatt ggggctatga ctgtcgtact ttttgcccct gaagatcttc
    22921 aattagttaa aggtgcacct agtctaagac gaaaattttt agatatagat attggacaaa
    22981 ttaagccaac gtacttagct gaactttcaa attacaatca tgtattaaaa caaagaaaca
    23041 cttatctaaa aactactaat aacgtcgaca aaactttttt aacagtcctt gatgaacaat
    23101 tagctgatta tggaagtcgt gtcattgaac accgttttga ttttatccaa gccctaaatg
    23161 atgaagcgga caaacatcac tacattatca gtactgagtt agaacattta tctattcact
    23221 acaaatcgtc aatagagttt acagataagt caagtattag ggaacatttt ttaaaccaat
    23281 tatctaaaag ccactcaaga gacatcttta agaaaaacac aagcatcgga cctcatcgtg
    23341 atgatattac tttctttatt aatgatatca atgctacttt tgctagccaa gggcaacaaa
    23401 gaagccttat tttatcattg aaattagcag aaattgagct aataaaaacc gttaccaacg
    23461 attatccgat tctcttactt gacgacgtca tgagtgaact tgacaaccat cgtcaactaa
    23521 aactcttaga gggaatcaaa gagaatgttc aaacatttat tactacaaca agtttagaac
    23581 atttaagtgc tttacctgat caactcaaaa tctttaacgt tagtgatggt actattagta
    23641 ttaatgaaaa aaaagcaaca gattaatctg ttgcttttta ataagattta acaataccaa
    23701 gtaatatagc acccataaca aaacaaagga tacccatgac aacccacttc tgctctttct
    23761 tcgtttttgt ctcacctaaa aataaaatac caccaataat agagataatt actccaagtt
    23821 gagaaaaact aaaagcaatt gctagccctg ctttagctgc tgccaataac atgaagacat
    23881 taccaacgcc ccacatgaga cctgtaatca tatttttaac aacaacagcc tcaaagttaa
    23941 cacgaaactt catgaaacaa attgcaccta gacacattcc aacagccatg ggtaaaataa
    24001 cggccatagc gtcgaacttc ataatgttat taaataaaac tgcgtatgag atgtaaccta
    24061 cagttgaata agcaattgta gcaaatcctt ttgaaaactc ttgatgtgtt gccaaagctt
    24121 gttctgagac atcacgttta cttgagaaat agaagccgat aactaataat gtcaacgctg
    24181 tcaatcctaa aataaattgg attggcttag tccattcatg aaagactaaa gcaccaacta
    24241 ggcttccacc tactaattgt gcaccacttg acagtggatt agcaacagag actcccatat
    24301 atttcattgc ttggaattga ccattttggc cgactgacca taggatacca cctaagatac
    24361 caaaaatcca caatgaggca gtcatctctg gctgtttaaa taaccatacg ataatcgcaa
    24421 atagcaatgc tcctaaagtc attccaaatg tttgttgatt tggacgtcct ccaattttat
    24481 tactaacaaa tccaatactt ccccacgcaa acatgggaat caatgcaata agtaaacctt
    24541 ccaattcttt ctccttacaa tggaatatta agtattacct attttcaatc ctagagaatc
    24601 tattgactaa ctttattagc aagatcagca aatactatag ctttatcaac actatctttt
    24661 tccaaagcta taatgacttg ttttatcaat aaattgacca gtgatttaga ataaggtaac
    24721 tgttctttag tcatattact aatcttattt aaaataacat cttcaggtaa aatatcttga
    24781 aaatagcaga tgactttgaa ataaaaatca ttgtactgtg tcacaccatt cagataagta
    24841 actaccaggt taatctcatc ttgaggcagc ctcatattta accctaaatt tgaaataaaa
    24901 agtttagcca atattttgag aacattgctt tctattctat cacaggattg ttgtttttgg
    24961 aaactgaaaa ttctcatgat atcttcatta tctcgataag attccagtaa agttaattgc
    25021 tctaaatcag tattagaagt tccagcctta acttttgaat ctagaatgga tacttcattg
    25081 atagttaccc caacctccaa cgtttgtaaa gctttaataa atttatcaat acctatttgt
    25141 gtctcaccat tttcaaaacg tgatagagtt gattttgata attgagcact tttggctaga
    25201 gaacttaggg atataccttt gtcctctctc aactctttta aaatttttcc ataatttttc
    25261 atacaaaaac tttactatat ttgtttcttt tatgcaacaa aaagcatcct cataggggat
    25321 gctttttgcc cttattttag tgtactgaat aatttggtgc ctcatttgta atttgaacat
    25381 catggggatg gctttctttc aagccagctc ctgacatttc aacaaattga gcattatcat
    25441 gaagttcttt tatgttcgct gcacctacat atcccatacc agagcgaatg ccaccaagca
    25501 tttggaaaac aatatcggct actgaacctt tgtatgctac acgaccttca ataccttctg
    25561 gaaccaattt gttagcctcg tttacagaac cttggaaata acgatcgctt gaaccttttt
    25621 tcattgctgc aattgagccc ataccacggt atgtcttgaa cttacgccct tggaaaattt
    25681 ctgtttctcc tggcgcttca tctgttcctg caaacattga acctagcata actgcatttc
    25741 cacctgctgc aagtgctttt acaatatcac ctgaatattt aataccacca tcagcaatga
    25801 tagtcttacc gtactcacgc gcaacagccg ctgcatcgta aatagctgta atctgaggaa
    25861 cacctacccc agcaacgaca cgagtcgtac aaattgaacc tggaccaata ccaactttaa
    25921 cgacatccac acctgcatca taaagtgcac gcgctccttc tgctgtagca atatttcccg
    25981 caatcaatgt gcgattaggg aagtgagcac gaatttcagc aattttacgg agcacaccag
    26041 ctgaatgtcc atgtgctgta tcaatgacaa tagcatctgc acctgcttca aacaaagctt
    26101 ctgcacgttc aaaagtatca gaggttacac ctactgcacc tgcaactaag agacgtccaa
    26161 attcatcttt tgctgcctta ggaaattcaa taactttttc gatatcctta atagtaataa
    26221 gtcctgaaag acgtccctca tcatctacta aaggtagctt ttcaatacga tgctcatgaa
    26281 gaatacgctc tgcagtttca agatctgtac caataggagc tgttacaaga ttttgacttg
    26341 tcatgtgctc tgagattagt tgtttataat cagaaatgaa acgcatatca cggttagtaa
    26401 taattccaac taatttacga ttctcgagtg tctctacaat aggaactcca ctaatgcgat
    26461 agttttgcat caattcctca gcttcagaga cagtattatc tggtgtcaag aagaatgggt
    26521 caataataac accattttct gagcgtttaa ctttacgaac ttcttctgct tgatcaacta
    26581 ttgacatatt tttatgaatg ataccaagcc caccagcacg tgcaatagca atagccatct
    26641 tactatctgt aactgtatcc attgcagctg taatgattgg aatgtttaat gtcaaattat
    26701 cagccaactt tgtcttcata tcaacttcat ttggtaaaac atggctttca gcaggaataa
    26761 gcagaacatc atcaaaagta aaaccttttt tcaaaaactt agtatcccaa tttgacatca
    26821 aaaaatcctc ttttcttaga tagtagatat aaaaaatccc attaaaatta tattgttact
    26881 atcatatcac gctaaaatca tttgtcaata taaaaagagc atagaattaa tagcttctag
    26941 ctctttaaag taatgaaaaa atcttcttaa ttttctattt ttttgagaag aagaaaggcg
    27001 gcgtatactg acttaatttc tcaaaacact tttgagcttc ttcactatta ttacaaataa
    27061 tcaaacaagt atcatcacca cagacagttg ctaagatctc aggtaataaa atcgaatcta
    27121 atatggagcc aaaagattga gctaagccag gtagtgtctt caaaacgatt tggttttgaa
    27181 ctggaaacag catgactagg gcatcttcca tgtaaaaacg taaccgttct tcccaacgct
    27241 tctgagatat actatgaatc tcataataag tttctgtagc attatcactg ctttcgttaa
    27301 ccttaattaa attaagttcc ttcatatctc gagataatgt tgcttgcgtc actgaaacgc
    27361 catttttttc tagtaattcc cttaactcat gttgtgtacg caccttagtc tcagagacta
    27421 aggaacgaat taattggtgt cttgtttctt ttttattcat ataaactcct tattcaatga
    27481 atttcttgaa ataattttta ttattataga gaagcttctt tcctttcatt tcaacgatat
    27541 ctttttggcg taattccttt aaaacatgac taactgtctc gcgagtagtt ccgctcatat
    27601 tggcaatttc aatcgttgta ataggaaaag gtaaatgtcc cctttctgta ggaacttgca
    27661 aaagtagagt tgcaagcgat tggataactc tcatactggc acttgaagtc accatattac
    27721 gaagtcgtat ttcatgaact cgtaatagct tagaatattt ccgacaaaga tgcttcattt
    27781 gattgatatt ttgaagagag tattcttcgt ataaagccat tggcagagaa aaatactcaa
    27841 tatccgtaat agccactgct gaaaaatggt aatcatcatc caaaaagaga cctccataag
    27901 gaaaaaccgt accttgtcta acataatctg tataaatgaa agaaccagaa gcgtctgatt
    27961 gttcaatttt tacatgtcct gactggatta aaaacagttt ttcacggtaa tcaccttcaa
    28021 aaaataaggt atgatttttt agagctgtac gatgtttaat gtggctcaca atatggtcaa
    28081 attgttcaat agtaaagtgt ttaaaatcat ctagttgtct gaaataaaaa tattgttctt
    28141 ttgtaatcac ctgattttct agacctttct aatcattaga tgcgaacatt ttttgtataa
    28201 tgaaaatttt t
//
LOCUS       DATRMA010000020        27964 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.020, whole genome shotgun sequence.
ACCESSION   DATRMA010000020 DATRMA010000000
VERSION     DATRMA010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 27964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27964
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.020"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            complement(<1..654)
                     /locus_tag="VBC28_000824"
     CDS             complement(<1..654)
                     /locus_tag="VBC28_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEO6814825.1"
                     /translation="MDKEKLDYWKTIITFLHNVLGDNYEIVLHVVDENDIYIGELVNS
                     HISGRTISSPLTTFALDLIKNKVYKEKDFVTNYKAIVSPLNKEVRGSTFFIKNAQNEL
                     EGMLCINLDISAYQNIALDILDLVNLNVNKILPKSPQKISLPQQEEPVEVLSGNIQDI
                     ISEIVDPSLLNQNIHLSQEVKVEIVSKLHEKGVFQLKGAVSKVAEVLNISEPSVYRYL
                     "
     gene            1016..2038
                     /locus_tag="VBC28_000825"
     CDS             1016..2038
                     /locus_tag="VBC28_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEO6814826.1"
                     /translation="MNIIIGTSLLILVLAIFTLFNYKAPYGTKAMGALASAACASFLV
                     EAFQDSFFGKVLGFQFLSEVGGANGSLSGVAAAILVAIAIGVTPGYAVLIGLSVSGTG
                     IIPGFLAGYLVGFLVKWMERNIPGGLDLISIIIIGAPLTRLVAKLLTPLINSTLLTIG
                     DILTSGAHSNPILMGIILGGTIVVVATAPLSSMALTAMLGLTGMPMAIGALSVFGSSF
                     MNGVLFHKLKLGSRKDNIAFAVEPLTQADVTSANPIPIYVTNFVGGAACGILIALMKL
                     VNDTPGTATPIAGFAVMFAYNPMIKVLITALGCIILSLLAGYFGGIVFKDYKLVTKEE
                     LQARDN"
     gene            2409..3701
                     /locus_tag="VBC28_000826"
     CDS             2409..3701
                     /locus_tag="VBC28_000826"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000205037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEO6814827.1"
                     /translation="MTSVVVVGTQWGDEGKGKITDFLSADAEVIARYQGGDNAGHTIV
                     IDNKKFKLHLIPSGIFFKEKISVIGNGVVVNPKSLVKELAYLHGEGVTTDNLRISDRA
                     HVILPYHIKLDQLQEDAKGDNKIGTTIKGIGPAYMDKAARVGIRIADLLDREVFAERL
                     KINLAEKNRLFEKMYDSTPLEFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDEVGDRIREIGKEYGTTTGRPRRVGWFDSVVMRHSRRVSGITNLSLNSIDVL
                     SGLDTVKICVAYDLDGKRIDYYPASLEQLKRCKPIYEELPGWSEDITACRSLDDLPEN
                     ARNYVRRVGELVGVRISTFSVGPGREQTNILESVWSNI"
     gene            complement(3724..4017)
                     /locus_tag="VBC28_000827"
     CDS             complement(3724..4017)
                     /locus_tag="VBC28_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000600818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6814828.1"
                     /translation="MILINLIALPICFLVELSAGLLLKLEMHKQHGYYPLERLLMTGN
                     ILTVVSLVLLHTLIHNINSLLFLSITVVLLISGKSLSLISIYLLKRKSKHQKV"
     gene            4356..4973
                     /locus_tag="VBC28_000828"
     CDS             4356..4973
                     /locus_tag="VBC28_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017772020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6814829.1"
                     /translation="MRKFFQDVIVEVAYYLEIALSVILATAIIFFSLALFHDLFSMLS
                     VDQNVDKLFQTFLARAMTIAVGIELIKMFSKPSPDTLIEVLLFALARQLVIEHGSVSD
                     YLMGIASVAILFAVRKYLFTHFDGSSNIVLRANNKVKKANILARVQLPCQPNETLGDL
                     LVRHLNDEEKDVAIGSVVYINNVALRVDSMHDGLVTRVEIIRSLH"
     gene            5141..6580
                     /locus_tag="VBC28_000829"
     CDS             5141..6580
                     /locus_tag="VBC28_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEO6814830.1"
                     /translation="MENFLAPLNWFSQNILQNPAFFVGLLVLIGYLLLKKPLHDVFAG
                     FIKATVGYLILNVGAGGLVNTFRPILVALAKKFNLEAAVIDPYFGLASANAKLETMGF
                     ISVATTALLIGFGINILLVALRKVTKVRTLFITGHIMVQQAATISVFVLLLIPQLRNG
                     FGAWAVGIICGLYWAVSSNMTVEATQRLTGGGGFAIGHQQQFAIWFVDKVAPFFGKKE
                     ENLDNLKLPTFLNIFHDTVVASATLMLVFFGGILAVLGPDIMSNVKLIGPGAFVPTKQ
                     AFFMYILQTSLTFSVYLFILMQGVRMFVTELTNAFQGISNKLLPGSFPAVDVAASYGF
                     GSSNAVLSGFAFGLIGQLITIALLVVFKNPILIITGFVPVFFDNAAIAVYADKRGGWK
                     AAVALSFISGIIQVALGAVAVGLLGLAGGYHGNIDFEFPWLAFGYIFKYLGIAGYVIV
                     CLFFLAIPQLQFMKSKDKEAYYRGDAPAE"
     gene            6608..6886
                     /locus_tag="VBC28_000830"
     CDS             6608..6886
                     /locus_tag="VBC28_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEO6814831.1"
                     /translation="MVKVLTACGNGMGSSMVIKMKVENALRQLGVSNFESASCSVGEA
                     KGLAANYDIVVASNHLIHELDGRTKGHLVGLDNLMDDNEIKTKLQEIL"
     gene            6953..7438
                     /locus_tag="VBC28_000831"
     CDS             6953..7438
                     /locus_tag="VBC28_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEO6814832.1"
                     /translation="MNLKQAFIENDSIRLKLSASDWKEAIKLSIDPLIESGAVDAEYY
                     DAIIESTEEFGPYYILMPGMAMPHARPEAGVKRDAFSLITLTEPVVFPDGKEVSVLLA
                     LAATSSAIHTSVAIPQIIALFELENSIQRLTECQEAKEVLAMVEESKNSPYLEGLDLE
                     S"
     gene            7551..8216
                     /locus_tag="VBC28_000832"
     CDS             7551..8216
                     /locus_tag="VBC28_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HEO6814833.1"
                     /translation="MTKRLPNLQVALDHSDLQGAIKAAVSVGHEVDVIEAGTVCLLQV
                     GSELVEVLRSLFPDKIIVADTKCADAGGTVAKNNAVRGADWMTCICCATIPTMEAALK
                     AIKEERGDRGEIQIELYGDWTYEQAQQWLDAGISQAIYHQSRDALLAGETWGEKDLNK
                     VKKLIDMGFRVSVTGGLSTDTLQLFEGVDVFTFIAGRGITEADDPAAAARAFKDEIKR
                     IWG"
     gene            8220..9083
                     /locus_tag="VBC28_000833"
     CDS             8220..9083
                     /locus_tag="VBC28_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HEO6814834.1"
                     /translation="MTRPIGIYEKATPKHFNWLERLQFAKELGFDFVELSIDESDERL
                     ARLEWSKEERLELVKAIFETGVRVPTITFSGHRRFPMGSNNPEKEARAMDMMKKCIVF
                     AQDIGIRNIQLAGYDVYYEEKSPETRARFIKNLRQACTWAEEAQVILSIEIMDDPFMN
                     SIEKYLAVEKEIDSPYLFVYPDTGNVSAWHNDLWSEFYNGHRSIAALHIKDTYAVTET
                     SKGQFRDVPFGQGCVDWEEMFAVIKKTNYNGPFLIEMWSENCETVEETRAAIKEAQDF
                     LYPLMEKTGVK"
     gene            9085..9801
                     /locus_tag="VBC28_000834"
     CDS             9085..9801
                     /locus_tag="VBC28_000834"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001934215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HEO6814835.1"
                     /translation="MAKSLQEMRERVCEANKSLPVHSLVKFTWGNVSEVDREAGLIVI
                     KPSGVDYDQLTPENMVVTDLEGNIVEGDLNPSSDLPTHVQLYKAWPEVGGIVHTHSTE
                     AVGWAQAGRDIPFYGTTHADYFYGPVPCARSLSEDEVNTAYEKETGSVIIEEFERRDL
                     DPMAVPGIVVRNHGPFTWGKDPAQAVYHSVVLEEVAKMNRFTEQINPRVEPAPKYIMD
                     KHYLRKHGPNAYYGQKGQEH"
     gene            9812..10459
                     /gene="fsa"
                     /locus_tag="VBC28_000835"
     CDS             9812..10459
                     /gene="fsa"
                     /locus_tag="VBC28_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEO6814836.1"
                     /translation="MKYFLDTADVSEIRRLNRLGIVDGVTTNPTIISREGRDFKEVIN
                     EICQIVDGPVSAEVTGLTCDEMVTEAREIAKWSPNVVVKIPMTEEGLAAVSQLSKEGI
                     KTNVTLIFTVAQGLSAMKAGATFISPFVGRLEDIGTDAYALIRDLRHIIDFYGFQSEI
                     IAASIRGLAHVEGVAKCGAHIATIPDKTFASLFTHPLTDKGIETFLKDWDSFKKK"
     gene            10623..11606
                     /locus_tag="VBC28_000836"
     CDS             10623..11606
                     /locus_tag="VBC28_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEO6814837.1"
                     /translation="MFEKVTISDIANLVGVSKATVSYYLNGNYKKMSLQTKEKIRLAI
                     KETGYQPSKIAQSLVTKNTRTIGVVIADITNPFISSVMKGIHDTCQQFGYSVNFTNSD
                     NDIDIELENLNRLNQQNVSGIILDSVDPNHSFIETLSNDRLVMVDRQAKDIKVDTVAS
                     DNKESTQIFLEKMQEAGYHDIYFVTYPIEGISTRELRYEGFKEVVSSNPDKLIIITED
                     GSTQRILDIIEHSEQKPGFLMMNGPTLLNFMKKLNQSTVSYPEDYGLGSYEDLEWMQV
                     LTPNVSCIKQDSYGIGCLAAQCLIEKISQGNEPTTARLLEVKNQIVIRQSF"
     gene            11841..12860
                     /locus_tag="VBC28_000837"
     CDS             11841..12860
                     /locus_tag="VBC28_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6814838.1"
                     /translation="MKINKLLSDRLEACYSVCRLQNQDYDFLLVASEVDQACFAYDLN
                     SDLAKIVVWEDVGGTMSIIPIPGTMDFLATQKFYPGFKAKDCQVVYASFQGDHWDICN
                     AFDFPYLHRFDLIKGKENQLYFIGCTIANSKAHTEDWSDKGRIFVARFNTQNHTLEGL
                     QQLPQTLLKNHGYYAIQDEGYSLITSVEGVFKLTYPEFSTTGDWQLERLFDEETSDVV
                     KVDINQDGKDEYVIIQGFHGDRLRIFTEDFGRELFHYPEKTPFGHAIWSGRLLNQTCF
                     VFGWRSEKAELRLFHFVDGHLVSELVDAKAASSNVLAFEKDGKAYLFSANNGRGEVAL
                     YQLVK"
     gene            12879..13835
                     /locus_tag="VBC28_000838"
     CDS             12879..13835
                     /locus_tag="VBC28_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017766789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEO6814839.1"
                     /translation="MDKKKILVTGIVPKEGLRKLMDRFDVTYSEDRPFSRDYVLEHLS
                     EYDGWLLMGQKGDKEMIDAGENLQIISLNAVGFDHVDTAYAKEKGIIVSNSPQAVRVP
                     TAEMTFALILAASKRLAFYDSIVRSGEWIDPSEQRYQGLTLQGSTLGIYGMGRIGLTV
                     ANFAKAFGMTVVYNDVYRLPEDKEKELGVTYLEFDQLIKTADVITIHAPALPSTIHKF
                     NKDVFAKMKNRSYLINAARGPIVSEEALIEALKEGEIAGAGLDVFENEPQVSEGLRSL
                     DNVIMSPHAGTGTIEGRRTLAEEAADNIIAFFDGKPQNIVNK"
     gene            13933..15384
                     /locus_tag="VBC28_000839"
     CDS             13933..15384
                     /locus_tag="VBC28_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000888340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HEO6814840.1"
                     /translation="MLEALHKFFEVFGAATVVPAMIFIVSLFLKVNPRRAFFSALRAG
                     VGLTGFGWIISAFAPMVTKYIQQMVKTTGLHLPIVDIGWQAGSLTAFSSEIGLSFFVF
                     GLLIELGLFLLGITRVFVPSNLWNNFGYMIWGTMAYAATGNFILSFAFMVFVLLYSLV
                     MSEVLADRWSEYYGVKNATINSIHNIETLIPALILDPLWNLLGVNKVKLNPESLKTKL
                     GIFGEPMTLGFILGVIIGVLGSLRNLASIDTWGGILGFAVALAAVMTIFPLITGVFAS
                     AFAPLAEAVERNKKKESQAEQGALDKKRWFIAVDDGVGFGEPATIIAGLILVPIMVVI
                     SLILPGNEALPVVDLIAIPFMIEAMIAVSKGNILKAILNGIIWFSLGLYAASALGPIY
                     TEAVKHFGTALPAGVTLIMSFNLLAHPLTALVFFAWISGNPLYIGVTVVIYLAFLFLL
                     RTHRESIYAYLKTMADKNSGYEGSCEKIFIAED"
     gene            15406..16395
                     /locus_tag="VBC28_000840"
     CDS             15406..16395
                     /locus_tag="VBC28_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEO6814841.1"
                     /translation="MKKIDGHAHIVDTIAGFNGKGRLNALGNGYAIWDDGDLIKLIPD
                     EYGDKAFTAEAFLKYMDANGVDKAVILQGHLNGYQNYYTHLAIKRYPERFTGAFSVDP
                     FADNAMQIVKRHVEVLGFRAIKFEISQGGGIHGYRGQKTPFRLDTDPHVSRILTYLLD
                     YPGFVVTVDYGNWDQISHQPDAIANLARLYTSLDFVVCHLSFPHVEHSNRLRAELNMW
                     KNFNNIYTDISAIQDIDAPDSFPFPKSEQNVRIAKEVLGAKRIIWGTDSPWSATFNTY
                     EELATWLENVDIFSQEELEDVMYNNAERVYFKEEHVKANRAGIDDTTKELNLY"
     gene            16461..17978
                     /locus_tag="VBC28_000841"
     CDS             16461..17978
                     /locus_tag="VBC28_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HEO6814842.1"
                     /translation="MTYYLSIDYGGTNTKALIFDKLGHQIAVSSFETLKNETQSGHRQ
                     VNLVKTWNAITSAIREVIQISKLSPEQISAVACIGHGKGLYLLDNKLEPLEQGILSTD
                     NRAKDLAQYFESKLDNIWELTRQHIFPSQSPVILRWLKDYQPETYKSIGAVLSAKDFI
                     RYKLTGKVQQEYGDASGNHWINFQTGTYDPAILDFFGIREIENSLPELIDSADLVPGG
                     ISSQAAKETGLVEGTPVVGGLFDIDACALGSGVLESDTFSVISGTWNINTYPSLKPAK
                     QDSGLMTSYFPDRRYLLEASSPTSAGNLNFMLKMLMHQEIDNAKSSGGSIYDNLEEFL
                     THTDATHHGLIFFPFLYGSNTSQDASACFFGLTTKSTKSQMIRAVYEGIAFAHKQHIT
                     DLIKSRGSVPKIIRFSGGATNSPAWMQMFSDILNFPIETVEGTELGGLGGAILARHAL
                     DKISLKEAVQDMVRVKAIYKPQLSEVKGYKKKYHAYQKLLETLDLIWSELGHLNK"
     gene            17998..18759
                     /locus_tag="VBC28_000842"
     CDS             17998..18759
                     /locus_tag="VBC28_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP endonuclease"
                     /protein_id="HEO6814843.1"
                     /translation="MKQEDIIINSIVFKKQLDKGFTQVQLIKLVKDLGFSRFEIRQEL
                     LQDPDRELPALKAEADFYDINLYYSANEDLIKGGKVNPYLNKGLKEASQLGAPFIKLN
                     VGQTRNLSKEELEPLKEILKSQTIGIKVENNQDPKAATVENCQYFMTLVKELQIPISF
                     VFDTANWAFINQDLYQAVNNLACDTTYLHCKNFIQVAGKPHLSKSLFEGEINLTDLLK
                     SFSNCEYLALEYPTELEILKRDVQRLISISNSQNF"
     gene            18825..20504
                     /locus_tag="VBC28_000843"
     CDS             18825..20504
                     /locus_tag="VBC28_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000584682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEO6814844.1"
                     /translation="MIILDKKSYDLLFYLLKLEEPETVMAIANALNQSRRKVYYHLEK
                     INDALPSDVPQIVSYPRVGILLTEKQKAACRLLLDEVTDYSYVMKSSERLQLSLVSIV
                     VAKDRVTIDRLMQLNDVSRNTILNDLNELRSELAEKEYNLQLQSTKCRGYFLDGHPLS
                     IIQYLYKLLDDIYHNGSSSFIDLFNHKLSQAFGASTYFSKEVLDYFHHYLFISQRSLG
                     KKINSQDGQFMIQILPFILMAYRKMRLSPEVQTSLNSDFSLVWQRKEYEIAKELADEL
                     EENFQLSLDEIEVGLVAMLMLSFRKDRDNHLESQDYDDMRATLTSFLKELEERYHLHF
                     VHKKDLLRQLLTHCKALLYRKRYGIFSVNPLTEHIKDKYEELFAITSSSVKLLEKAWQ
                     IKLTDDDVAYLTIHLGGELRNSQQSPNKLKLVIVSDEGIAIQKLLLKQCQRYLTNSDI
                     EAVFTTEQYQSVSDLMHVDMVVSTSDALESRFPMLVVHPVLTDDDIIRLIRFSKKGNC
                     ANSNQFTNELEKTIAQYVKEDSERYVLKSKIEKLIHQELLQDVLPLQSTVC"
     gene            20821..21912
                     /gene="ulaG"
                     /locus_tag="VBC28_000844"
     CDS             20821..21912
                     /gene="ulaG"
                     /locus_tag="VBC28_000844"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HEO6814845.1"
                     /translation="MPNVKDITRESWILSTFPEWGTWLNEEIEEEVVAEGNFAMWWLG
                     NCGVWIKTPGGANVVMDLWSNRGKSTKKVKDMVRGHQMANMAGVRKLQPNLRAQPMVI
                     DPFAINELDYYLVSHFHSDHIDINTAAAIINNPNLDHVKFVGPYECGEIWKKWGVPEE
                     RIIVIKPGESFEFKDIKVTAVESFDRTCLVTLPVDGAEEHDGELAGLAVTDEEMARKA
                     VNYIFETPGGTIYHGADSHFSNYFAKHGKDYKIDVAINNYGDNPVGIQDKMTSIDLLR
                     MAENLRAKVIIPVHYDIWSNFMASTDEILQLWKMRKERLQYDFHPFIWEVGGKYTYPQ
                     DKDRIEYHHPRGFDDCFEQESNIQFKALL"
     gene            22002..24380
                     /locus_tag="VBC28_000845"
     CDS             22002..24380
                     /locus_tag="VBC28_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HEO6814846.1"
                     /translation="MSEFDTKSYLEKLDAWWRAANYISAAQMYLKDNPLLRRELVEND
                     LKVHPIGHWGTVPGQNFIYAHLNRAINKYDLDMFYIEGPGHGGQVMVSNSYLDGSYTE
                     LNPNIEQTEDGFKQLCKIFSFPCGIASHAAPETPGSIHEGGELGYALSHATGAILDNP
                     DVIAATVIGDGEGETGPLMAGWLSNTFINPVNDGAVLPIFYLNGGKIHNPTIFERKTD
                     EELSQFFEGLGWKPIFADVVELSEDHAAAHALFAEKLDQAIQEIKTIQSEARQKPAEE
                     AIQAKFPVLVARIPKGWTGPKAWEGTPIEGGFRAHQVPIPVDAHHMEHVDSLLSWLQS
                     YRPEELFDENGKIVDEIAAISPKGDRRMSMNPITNAGIVKAMDTADWKKFALDINVPG
                     QIMAQDMIEFGKYAADLVDANPDNFRIFGPDETKSNRLQEVFTRTSRQWLGRRKPDYD
                     EALSPAGRVIDSQLSEHQAEGFLEGYVLTGRHGFFASYESFLRVVDSMVTQHFKWLRK
                     SKTHTTWRKNYPALNLIAASTVFQQDHNGYTHQDPGILTHLAEKTPEYIREYLPADTN
                     SLLAVMDKAFKAEDKINLIVTSKHPRPQFYSIAEAEELVAEGYKVIDWASNVSLNQEP
                     DVVFAAAGTEPNLEALAAISILHKAFPELKIRFVNVLDILKLRHPSQDARGLSDEEFN
                     KVFTTDKPVIFAFHGYEDMIRDIFFSRHNHNLHTHGYRENGDITTPFDMRVMSELDRF
                     HLAQDAALASLGNKAQAFSDEMNQMVAYHKDYIREHGDDIPEVQNWKWENIK"
     gene            24757..25980
                     /locus_tag="VBC28_000846"
     CDS             24757..25980
                     /locus_tag="VBC28_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HEO6814847.1"
                     /translation="MTNILEVKNLTKIFGKKQKAALEMVKQGKSKTEILEKTGATVGV
                     YDASFEIKEGEIFVIMGLSGSGKSTLVRMLNRLIDPSSGNIYLDGKDIAKMNVEDLRN
                     IRRHDINMVFQNFGLFPHRTILENTEFGLEMRGVSKEERTTLAEKALDNAGLLPFKDQ
                     YPSQLSGGMQQRVGLARALANSPKILLMDEAFSALDPLIRREMQDELLDLQDTNKQTI
                     IFISHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDFVREFVEDVDRSKVLTA
                     QNIMIKPLTTVLEIDGPQVALTRMHREEVSMLMATNRRRQLLGSLTADAAIEARKKDL
                     PLSEVIDKDVVTVSKDTVITDIMPLIYDSSAPIAVTDDNDRLLGVIIRGRVIEALANV
                     QDETVVESPKETVEA"
     gene            25999..27726
                     /locus_tag="VBC28_000847"
     CDS             25999..27726
                     /locus_tag="VBC28_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HEO6814848.1"
                     /translation="MENLLQHKLPVAPFVESTTNWITKTFSGLFDFIQTIGNALMDWM
                     TKTLLFINPLLFIVLITIAVFFLAKKKWQLPTFTFIGLLFIYNQGLWEQLINTFNLVL
                     VASLISIIIGVPLGIWMAKSDKVKQVVNPILDFMQTMPAFVYLIPAVAFFGIGMVPGV
                     FASVVFALPPTVRFTNLAIREIPLELIEASDSFGSTVKQKLFKVELPLAKNTIMAGIN
                     QTMMLALSMVVTGSMIGAPGLGREVLSALQHADIGTGFVSGLSLVILAIVLDRVSQFF
                     NSKPGEKQAKTSKVKKWVGLGALALFILAALGRIVVNMTSGNEAKGQKVKIAYVQWDS
                     EVASTNVIAEVLKSKGYDVELTPLDNAVMWQTVANGNADFTTSAWLPKTHGQYFNKYK
                     NSLDDLGPHVENVKIGLVVPKYMNVNSIEELSNQADKQITGIEPGAGIMKSAKQSLKD
                     YPNLSSWKLLSASTGAMTTTLGKAIKNKDQVVITGWSPHWMFAKYDLKYLKDPKKSFG
                     GEEHINTIARKNLKKDMPKVYKIIDKFKWTKEDMESIMLDMDKGMEPAKAAQKWIKNH
                     KKEVSEWTK"
BASE COUNT         8847 a         4566 c         5557 g         8994 t
ORIGIN      
        1 aagataacga tagacgcttg gttcggagat gttgagaact tcagcgacct ttgaaacggc
       61 accttttaat tggaatacac ctttttcgtg aagtttagaa acaatttcta ccttaacttc
      121 ttgacttaaa tgaatattct gatttaaaag actaggatca acaatctcac taataatatc
      181 ctgaatattg cctgatagaa cttctacagg ttcctcctgt tgagggagag aaattttttg
      241 aggggactta ggtagtattt tgttaacatt taaattaact aagtctagaa tatccaaagc
      301 aatattttgg tatgcagaaa tatcaaggtt gatacataac atcccttcaa gttcattttg
      361 agcattcttg atgaagaaag tagatccacg aacttcttta ttgagagggc tgacaatcgc
      421 cttatagtta gtaacaaaat ctttttcctt ataaacttta ttcttgatta aatcaagggc
      481 gaaagtcgtt agaggtgaac ttatggttcg tccactaatg tgactattaa ccaactcacc
      541 aatatagata tcattttcat ctactacatg caaaacaatt tcgtaattat cacctaaaac
      601 attatggaga aacgttatta tcgttttcca atagtctaat ttttctttat ccatatcgta
      661 gttcctctat gctaatctta ctattatagt aatatattta tggttaaaag acaatatcag
      721 actattttaa aagattaatg taataaaaaa ttatcgtaat aaaaaaaagt taagaaaata
      781 aaaaattatt attgactatg gttatgcttt taggtaaaat gtgattatat tagcaactaa
      841 ggagtaatcc atgagataag cctaataatt atgtcggact tctttgttgc accttatcca
      901 gttcgtagta caattaagtt tcagtattac ctaaaggcgc ctttattgaa attgaagcag
      961 tagtacacag acactgagaa taaaaaactt aactattttt agcgggagta tatttatgaa
     1021 tatcattatt gggaccagtc ttttaatact tgtattagcg attttcactt tatttaatta
     1081 taaagcaccc tacggaacaa aagcaatggg agcattggca tctgcggctt gtgcatcctt
     1141 ccttgtagaa gcttttcaag attctttctt tggtaaagtt ttgggatttc aatttttaag
     1201 tgaagtgggt ggtgctaatg gttcactttc tggtgtagct gcagctatat tggttgctat
     1261 tgctattggt gtaaccccag gatacgcagt tttaattggt ttatctgttt ctggaacagg
     1321 tattattcct ggtttccttg caggatattt agtgggattt cttgttaagt ggatggaaag
     1381 aaatattcct ggtggtttag atcttatttc aattattatt attggtgcac cattaacacg
     1441 tcttgtggcc aaactgttga cgccgcttat taatagcact ttgctcacca ttggagatat
     1501 cttaacatca ggtgctcata gcaaccctat tttaatgggg attatacttg gcgggacaat
     1561 tgtagtagtg gcgacagcac cactttcttc tatggcattg acagctatgc taggattaac
     1621 cggaatgcct atggctatag gagccttgtc tgtctttggt tcgtcattta tgaatggtgt
     1681 acttttccat aaattaaaac ttggaagtcg taaagataat atagcttttg ctgttgagcc
     1741 tctaactcaa gctgacgtga cttcagctaa ccctattcca atctatgtca ctaattttgt
     1801 tggtggtgca gcttgtggta ttttaattgc cttgatgaaa ttagttaatg atactcctgg
     1861 aacagcgaca ccaattgcag gatttgctgt catgtttgcc tataacccaa tgataaaagt
     1921 actaataacc gctctaggtt gtattatcct atctttacta gcaggctatt ttggaggcat
     1981 tgtttttaaa gattataaat tagttacaaa agaagagcta caggctagag ataactagtt
     2041 acttcatgaa aagctaatag taaatatcat taatatagat atgatatctg acttgtgata
     2101 actgttcttg ataatttaaa attcaacgac tctaataaaa agatcgttga atttttttat
     2161 tgttaagaat gctggtatca gtaaatttca agatttcttt atcaactata atgttagttt
     2221 tttaccgttt ttttacactt ctaataatta tttttttctt tttttccgat aatatttgag
     2281 gaatttggca aaaaaaaaca ttttttgcta taataaggtg tcagacaaac tcgcaggtga
     2341 aattcctgcc atgtttttcc agaaaggatt ggggagtccc aaaaaggctc actcagtgtt
     2401 aaataaatat gacttcagtt gttgtagtag gtacccaatg gggtgacgaa ggtaaaggta
     2461 aaattacaga ttttttatct gctgacgcag aagtaattgc acgttatcaa ggtggtgata
     2521 acgctgggca tactatcgtt attgataaca aaaaatttaa acttcattta attccttcag
     2581 gaattttctt caaagaaaaa atttctgtca ttggtaatgg tgttgtcgtt aatccgaaat
     2641 cattagttaa agagttagct tatcttcacg gagaaggtgt aacaacggat aaccttcgta
     2701 tttcagaccg tgcgcatgtt atcttgccat accacatcaa gcttgaccaa ctacaagaag
     2761 atgctaaagg tgataataaa attggaacaa ccatcaaagg aattgggcca gcttacatgg
     2821 acaaggctgc gcgtgtcggt atccgtattg ctgaccttct tgatagagaa gtttttgctg
     2881 agcgtttgaa aattaattta gctgagaaaa atcgtttatt tgaaaaaatg tatgacagta
     2941 cacctcttga atttgatgat attttcgaag aatattatga atatggtcaa caaattaagc
     3001 aatatgtgac tgatacatcc gttatcttaa atgatgctct tgatgccggt aaacgtgttc
     3061 tttttgaagg agcacaagga gttatgctcg acattgatca aggaacatac ccatttgtaa
     3121 catcttccaa tccagtagca ggtggtgtca caattggttc gggagttgga ccaagtaaaa
     3181 ttaataaagt agtaggtgta tgtaaagctt acactagccg tgtcggtgat ggaccattcc
     3241 caacagaact ttttgatgag gttggtgacc gtattcgtga gattggtaaa gagtatggta
     3301 caacgactgg tcgtcctcgt cgcgttggat ggtttgattc tgttgttatg cgtcacagcc
     3361 gtcgagtatc aggtattact aatctctctc tgaattcaat tgatgttctt tcagggcttg
     3421 atacggtgaa aatttgtgtg gcttatgacc ttgatgggaa acgtattgac tattatccag
     3481 caagccttga acagctaaaa cgttgtaaac caatctatga agaattaccg ggctggtctg
     3541 aagatattac agcttgccgt agcttagatg atcttccaga aaatgcacgt aattacgttc
     3601 gccgtgttgg cgaattggtt ggtgttcgta tttctacttt ctcagtaggg cctggtcgtg
     3661 aacaaacaaa cattcttgag tctgtttggt caaatattta atattaaaag tctgactgta
     3721 cagtcagact ttttgatgtt tactttttct ttttaataga tagatactaa taagacttaa
     3781 ggacttccca gaaatcaata aaactactgt aattgataaa aaaagaagtg aattgatatt
     3841 gtgaatcaaa gtatgtaata atactagcga tacaactgtt agaatattac cagtcatcaa
     3901 taagcgttcc agaggatagt agccatgttg cttatgcatt tctaatttta atagtagacc
     3961 agctgacaat tctactagga aacaaatagg tagagctatc aaattaataa gaatcataga
     4021 ccaaccaaat ccttgaccat aacttatata tgatagacta tttttggaaa ttgttgacgt
     4081 gtgaattata aaatcaatcg taaagataat gtctataaga agtagtagcg ctaacagata
     4141 atgtagtaag gagattgttc ttaaccgttt attaattaat atttcatttc ctcccactta
     4201 tttaccaata agcttattat atcataatgt gtaatcaaac attcgttagt actgattaaa
     4261 aggttaatga aggggtggtc actattgttt cattgaatta gtaactttaa cgacgaaatg
     4321 ttataatgtt attagtttat aaaagaggag attgtatgcg taaatttttc caagatgtta
     4381 ttgtagaggt ggcttattat ttagagattg ctttgtcagt tattctggca acagctatta
     4441 ttttttttag tttagcactc tttcatgatt tgtttagcat gttgtctgtt gatcaaaatg
     4501 ttgataagtt gttccagact ttccttgctc gcgccatgac aatagcagtc gggattgagt
     4561 tgatcaaaat gttttcaaaa ccttccccag atactttaat tgaagtgtta ttgtttgcct
     4621 tagctcgtca acttgtaatt gaacatggta gtgtttcaga ttaccttatg gggattgctt
     4681 cagttgctat tttgtttgct gttcgtaaat atcttttcac ccattttgat gggtcaagca
     4741 acattgtttt acgtgctaat aacaaagtca aaaaagctaa tattttagct agggttcaac
     4801 taccttgtca acccaatgaa actttaggag acttacttgt tcgtcattta aatgatgagg
     4861 aaaaggacgt tgctattggt tcagtagtct atattaataa cgttgcattg cgtgttgata
     4921 gtatgcacga tggtcttgtg acgcgtgtag aaatcattag gagtctacat taaaaaatat
     4981 aaaaaatcat gtatcctaag atacatgatt tttttgtaaa ttaggttcag cacaattttg
     5041 cacacttaag tgtgtacttc ttggtctttt ttaaaacgct tacaaaaaat ataataaaga
     5101 tacaaaaaaa cattacttaa ataaagagag gtctttagtt atggaaaact tcctagctcc
     5161 ccttaattgg ttctcgcaaa atatcttgca gaatccagcc ttcttcgtag gtctattggt
     5221 actcattggg tatcttttat tgaagaaacc acttcatgat gtctttgctg gttttatcaa
     5281 agcgaccgtt ggatatttga ttttgaacgt aggtgcagga ggattggtta atacattccg
     5341 tccaatctta gttgctcttg ctaaaaaatt taatttagaa gctgctgtta ttgaccctta
     5401 ctttggttta gcatcagcaa atgctaagtt agaaacaatg ggctttatca gtgttgcaac
     5461 aacagcactc ttgattggtt ttgggattaa cattctcttg gttgctcttc gtaaagtaac
     5521 taaagtccgt actcttttta ttactggtca tatcatggta caacaagctg caacaatttc
     5581 agtatttgta ctgctcttga ttccacaact tcgtaatggt tttggtgcat gggctgttgg
     5641 tatcatttgt ggtctatatt gggcggtaag ttctaatatg accgttgaag caactcaacg
     5701 ccttacaggt ggtggcggat ttgccatcgg acaccaacaa caatttgcaa tttggtttgt
     5761 tgataaagtt gcgccattct tcggtaaaaa agaagaaaac cttgataact taaaattacc
     5821 aacattctta aatattttcc atgatacagt tgttgcatca gcaacattga tgctagtttt
     5881 ctttggagga atcttagctg tccttggtcc agacattatg tctaatgtta aattaatcgg
     5941 acctggtgca tttgtaccaa ctaagcaagc tttcttcatg tacatccttc aaacatcatt
     6001 gacattctca gtttaccttt tcattttgat gcaaggtgta cgcatgttcg ttacagaatt
     6061 gacaaatgcc ttccaaggta tctcaaacaa acttcttcca ggatcattcc cagcagttga
     6121 cgttgcagct tcatatggtt ttggttcttc aaatgcagtt ctttcaggat ttgcttttgg
     6181 tttgattggt caattaatta caatcgctct acttgttgtc ttcaaaaatc caatcttgat
     6241 tatcacagga ttcgttccag tattctttga taatgcggca atcgctgttt atgcagataa
     6301 acgtggtggc tggaaagcag cagtagctct atcatttatc tcaggtatta ttcaggtggc
     6361 acttggtgct gttgctgtag gtcttttagg acttgcaggt ggttatcatg gaaatatcga
     6421 ctttgaattc ccatggttag catttggtta catcttcaag tatttaggta tcgctggtta
     6481 cgtgattgta tgtctcttct tcttagctat tccacaactt caatttatga aatctaaaga
     6541 taaagaagca tactatcgtg gtgatgcgcc tgcagaataa tagaaattta taacggagga
     6601 aataaaaatg gttaaagttc ttacagcatg cggaaacggc atgggatcat caatggttat
     6661 caaaatgaaa gtcgaaaatg cgcttcgtca attaggcgtt agcaactttg agtcagcatc
     6721 ttgctcagtc ggagaagcaa aaggtttagc tgcaaactac gatatcgtgg ttgcttcaaa
     6781 ccaccttatc catgaattag acggacgtac aaaaggtcat ttagtaggtc ttgataacct
     6841 tatggatgac aatgaaataa aaacaaaact acaagaaatt ttgtaagtta atagacaaag
     6901 ggagtgagac aaaagtttct ctcccatatc tttagacttt taaggagaaa tcatgaattt
     6961 aaaacaagcc tttatcgaaa atgactcaat tcgtcttaaa ttatctgcaa gcgattggaa
     7021 agaagcaatc aaattatcaa ttgatccatt gattgaaagt ggtgctgttg atgcagaata
     7081 ttacgatgcc attattgaat ctacagagga atttggtccg tactacattc ttatgccggg
     7141 tatggcgatg cctcatgctc gtccagaagc aggcgtaaaa cgtgatgcat tttcattaat
     7201 tacattaaca gagccagtag tttttccaga tggcaaagaa gtctcagtat tgttagcact
     7261 tgctgcaaca agttcagcta ttcatacttc tgttgctatc cctcaaatta ttgccctttt
     7321 tgaattggaa aattcaattc agcgcttgac ggaatgccaa gaagcaaaag aagtccttgc
     7381 aatggtagag gaatcaaaaa acagcccata tttagaaggt ttagatttag aaagctaatc
     7441 aataaactga tattaataga aaggatagga cctataaaaa atctctgtga taagaaactc
     7501 tttgccacaa ataaaatgag tatttttatc aggtccttag aataaacaac atgacaaaac
     7561 gtttaccaaa tttacaagtt gcacttgacc actcagattt acaaggagct atcaaagctg
     7621 ccgtatcagt aggacatgaa gttgatgtta ttgaagctgg aactgtttgc cttcttcaag
     7681 taggtagtga attagttgag gtccttcgca gtctcttccc tgacaaaata attgttgctg
     7741 atacaaaatg tgctgatgct ggtgggactg ttgctaaaaa taatgctgtt cgcggtgcag
     7801 actggatgac ttgtatctgt tgtgctacta ttccaacaat ggaagcagcc ttaaaagcta
     7861 tcaaagaaga acgcggcgat cgtggagaaa ttcaaatcga actatatggt gattggactt
     7921 atgagcaagc gcaacaatgg ttggatgcag gtatttcaca agccatttat caccaatctc
     7981 gtgatgcgct tcttgctggc gaaacatggg gagagaaaga ccttaataag gttaaaaaat
     8041 tgattgatat gggcttccga gtttctgtaa ctggtggttt atcaactgat actcttcaat
     8101 tatttgaagg tgttgatgtc tttactttta ttgcgggtcg tggtattaca gaggcagatg
     8161 atccagccgc agcagcacgt gcatttaagg atgaaattaa acgtatttgg gggtaatata
     8221 tgacacgtcc aataggaatt tacgagaagg caacaccaaa acattttaac tggttagaac
     8281 gcctacaatt tgctaaagaa ttgggatttg attttgtaga attatccatt gatgagtcag
     8341 atgaacgttt ggcacgtttg gagtggtcaa aagaagaacg acttgagttg gtcaaagcta
     8401 tttttgagac aggagttcgt gttccaacaa ttacatttag tggtcatcgt cgtttcccaa
     8461 tgggatctaa taatcctgag aaagaagcgc gtgcgatgga tatgatgaaa aaatgtatcg
     8521 tctttgctca agatattggt attcgtaata ttcaattagc aggatatgat gtgtactatg
     8581 aagaaaaatc tccagagaca cgtgcacgct ttattaagaa tcttcgtcaa gcttgtactt
     8641 gggccgaaga agcacaagtt attctatcta ttgagattat ggatgatcca tttatgaatt
     8701 ccattgagaa atatttggct gtggaaaaag agattgattc tccttatctc tttgtctacc
     8761 cagatactgg aaatgtatca gcatggcata atgatctttg gagtgaattt tataatggac
     8821 atcgctcaat tgcagcgctt catattaaag atacttatgc tgttaccgaa acgtctaaag
     8881 gtcaattccg tgatgttcca tttggtcaag ggtgtgttga ttgggaagaa atgtttgctg
     8941 tcattaagaa aacaaattat aatggtccat tcttgattga aatgtggtcg gagaactgtg
     9001 agactgtaga ggaaacacgt gcagccatta aagaggcgca agattttctt tacccattaa
     9061 tggagaaaac aggagtgaaa taatatggca aaatcattgc aagaaatgcg tgaacgtgtg
     9121 tgtgaagcta ataaatcact accagttcat agtttagtta aatttacttg gggtaacgtt
     9181 tctgaagttg accgcgaagc aggattgatt gttatcaaac cttcaggagt tgactatgac
     9241 caattgacac ctgaaaatat ggttgtcaca gacttagagg gaaatatagt ggagggggat
     9301 ttgaatccat catctgatct tccaactcat gttcaacttt ataaagcatg gccagaagtc
     9361 ggtggtattg ttcatactca ttcaacagaa gcagttggtt gggcacaagc gggtcgcgat
     9421 attccatttt atggaacaac acatgcagat tatttttatg ggccagtacc atgtgctagg
     9481 tcacttagtg aagatgaagt taacactgct tatgaaaaag aaacaggtag cgttattata
     9541 gaggaatttg aacgtcggga tttagatcca atggctgttc ctggtattgt tgtacgtaat
     9601 catggaccat ttacttgggg gaaagatcca gcacaagcag tttatcactc agtcgttctt
     9661 gaagaagttg ctaagatgaa tcgtttcact gaacaaatta atccacgtgt tgaaccagct
     9721 ccaaaatata ttatggataa acactattta cgtaagcatg gaccaaatgc ttattatggt
     9781 caaaaaggac aagaacatta aggagaataa tatgaaatac tttttagata ctgccgatgt
     9841 ttctgaaatt cgtcgtctta atcgtttagg aatcgttgat ggtgttacta ccaatccgac
     9901 tattatttca cgtgaggggc gtgactttaa agaagttatt aacgaaattt gccaaatcgt
     9961 tgatggccca gttagtgcag aagtgacagg tttaacttgt gatgaaatgg ttaccgaagc
    10021 tagagaaatc gctaagtggt caccaaatgt tgttgttaaa attccaatga ctgaagaagg
    10081 tttagcagca gtttcacaat tatcaaaaga aggtataaag acaaatgtga cattaatatt
    10141 tactgtggct caaggtcttt cggcaatgaa agctggtgca acatttatta gtccttttgt
    10201 gggtcgtctt gaagatattg gaacagatgc ttatgctttg attcgtgacc ttcgtcacat
    10261 tattgatttt tatggtttcc aatctgagat tattgctgct agcattcgtg gattggcgca
    10321 tgtggagggc gtcgctaaat gcggtgcgca cattgccact attccagata aaacatttgc
    10381 atcactattt actcacccgc ttactgataa agggattgaa actttcttaa aagattggga
    10441 tagtttcaag aagaagtagt gttacgataa agcaaactct taattatact agtgcaagac
    10501 aaactttgat aatgggtttg tcttttttta taatcttctc caataagaac acttattttc
    10561 atttttctta ttaaatgata taatacggat aaattaagtc taattagaat aaggaaatag
    10621 agatgtttga gaaggtaacc attagtgata ttgcaaattt agtaggtgtg tcaaaagcta
    10681 cagtttccta ttatctcaat gggaactata agaaaatgtc tctgcaaacg aaagaaaaaa
    10741 tacgtttagc tattaaggag actggttatc aaccaagtaa gattgctcaa agtttagtta
    10801 caaaaaatac ccgtactatt ggggtcgtta ttgctgatat tactaatccc tttatttcgt
    10861 ctgtgatgaa gggaatacat gatacttgtc aacaatttgg ttactcggtt aactttacta
    10921 actcggataa tgatattgac attgagttgg aaaatttaaa ccgtttaaat cagcaaaacg
    10981 tttcaggaat tattttagat tctgtagatc ctaatcattc atttatagaa actttgagta
    11041 atgatcgtct tgtcatggtt gatagacaag ctaaagatat taaagtagat actgtagcat
    11101 ccgacaataa agaatcgact caaatatttc ttgaaaaaat gcaagaagct gggtatcatg
    11161 acatctattt tgtcacttat cccattgaag ggatttcaac ccgtgagctt cgttacgaag
    11221 gttttaaaga agtggtcagt tcaaatcctg ataagttgat tattatcact gaagatggta
    11281 gtacacagag aattcttgat atcattgaac attcagagca aaaaccaggg ttcttaatga
    11341 tgaacggtcc aactcttttg aattttatga aaaaattaaa tcaatcaacg gtcagctacc
    11401 ctgaggatta tggtcttggg agttatgaag atttagaatg gatgcaggtg cttacaccaa
    11461 acgtttcctg tataaaacag gattcatatg gcattggttg cctggctgcc caatgtttaa
    11521 ttgaaaaaat tagtcaagga aacgaaccaa ctactgcaag gttactagaa gtaaaaaatc
    11581 aaatcgttat tcgtcaatct ttttaaaaat gtttgcgaga gcaagcattt ttttataaaa
    11641 aagcaaaaaa cactatgtaa acgcttgctt ttatagaaaa tgatagtata atagaattat
    11701 aaatgagtta accgtttaac ttaagaatag gtaagctttt aacgaaccat ctctaactct
    11761 cttaactcaa atgtttttat ttttttgcac cattagttaa acggttaact caagttttat
    11821 cttcaccaaa ggaggataga atgaagatta ataaattatt gtcagacaga cttgaagctt
    11881 gttattcagt ttgtcgtttg caaaatcaag actatgattt tttattagta gcttcagagg
    11941 ttgaccaagc ctgctttgct tatgatttga atagcgattt agcaaaaatt gttgtttggg
    12001 aagatgtggg aggtaccatg agtattattc caattcccgg aaccatggat tttttggcaa
    12061 cccagaaatt ttacccaggg tttaaggcca aagattgtca ggttgtttat gcaagttttc
    12121 aaggagatca ttgggacatt tgtaacgcat ttgattttcc gtatttacat cgctttgatc
    12181 tcattaaagg taaagaaaat caactttact ttataggttg tacaattgct aacagtaaag
    12241 cccacactga ggattggagt gataaaggcc gaatttttgt tgctcgtttt aatactcaaa
    12301 accatacatt ggaaggattg caacaattgc ctcaaacttt attaaaaaat catggatact
    12361 atgccattca ggatgaagga tattcattga ttacttcagt agaaggggta ttcaaactca
    12421 cttatccaga attttctact acaggcgact ggcaattaga acggcttttc gatgaggaga
    12481 caagcgatgt ggtgaaagtg gatattaatc aggatggcaa ggatgagtat gtgattatcc
    12541 aaggttttca tggagatcgt ttacgcatct tcactgaaga tttcggtcga gaattattcc
    12601 attatccaga aaaaacacca tttggtcatg ctatttggag tggtcgttta cttaatcaga
    12661 cttgttttgt attcgggtgg cgatcagaaa aagcagaatt aaggcttttt cactttgtag
    12721 atgggcactt ggtttcagaa ttagtagatg caaaagcagc ttctagtaat gtcttagctt
    12781 ttgaaaaaga tggaaaagct tatcttttct cagccaataa cggacgtggc gaagttgctc
    12841 tttatcaatt agtaaaataa atttaagaag gagacaatat ggacaagaaa aaaatcttag
    12901 taacgggtat tgtgcctaaa gaaggtctaa gaaagcttat ggaccgattt gatgttactt
    12961 attcagaaga tcgcccattt tcacgtgact atgtgttaga gcatttatct gaatatgacg
    13021 gatggttact catgggacaa aaaggcgata aagagatgat tgatgcaggt gaaaacttac
    13081 aaattatttc tttaaatgcg gttggttttg atcatgtaga tactgcgtat gctaaagaaa
    13141 aaggaattat agtctctaat tcccctcaag cagtacgagt tccgacagcg gaaatgactt
    13201 ttgctttaat tttagctgcc agtaagcgcc ttgcatttta tgatagtatt gtaagaagtg
    13261 gagagtggat tgatccgagc gaacaacgtt atcaaggttt gactttgcaa ggttcaacat
    13321 taggtattta cggaatggga cgcatcggtt tgacagtcgc aaattttgct aaagcatttg
    13381 ggatgactgt tgtttacaat gatgtttatc gacttcctga agataaggaa aaagagcttg
    13441 gtgtaaccta tctcgaattt gatcagctta ttaaaacagc tgatgtcata accatccacg
    13501 ccccagcatt accatcaaca atccacaaat tcaataaaga tgtttttgct aagatgaaaa
    13561 atcgaagcta cttaatcaac gctgctagag gcccaatcgt ttctgaagaa gcacttattg
    13621 aagcactgaa agaaggtgaa atcgcgggtg cgggattaga tgtttttgag aatgaaccac
    13681 aggtatctga aggattgaga agccttgata atgttatcat gagtccacat gctgggacag
    13741 gtactattga gggacgtaga actttggctg aagaagcagc tgacaacatc attgccttct
    13801 ttgatggcaa gccacaaaat attgtcaata aataattggc tagtgagcgc agagccatct
    13861 tttgaaaaaa tgggacctcc cattttttta cgggtaaatg taagcgctta ataatttaat
    13921 aaaggtgaac ttatgctaga agctttacat aaattctttg aagtctttgg tgcagcaact
    13981 gttgtacccg ctatgatttt tattgtcagc ctttttctaa aggtcaatcc aaggcgagct
    14041 ttctttagcg ccttacgagc tggtgttgga ctcacaggct ttggttggat tatatcagct
    14101 tttgctccta tggtgactaa gtatatccag cagatggtta aaactactgg tctccattta
    14161 ccaattgtag atattggttg gcaggctggt tctctcaccg ccttttcttc tgagattggc
    14221 ctctctttct ttgtttttgg cttgctaatt gaattggggc ttttcttgct tgggattacc
    14281 cgagtatttg tcccatctaa tctttggaat aactttggct atatgatttg gggtacaatg
    14341 gcctatgcag caacaggtaa ttttatattg tctttcgcat ttatggtttt tgtcttactc
    14401 tattcattag tgatgtcaga agtattggca gatcgatggt cggagtatta cggtgttaaa
    14461 aatgcaacga tcaactcgat tcataatatt gaaaccctta ttccagctct tattcttgat
    14521 cctctttgga acctacttgg tgtcaataag gtaaagttga acccagaatc attaaagact
    14581 aagttaggga tatttggtga gccaatgaca cttggtttta tacttggtgt gattattggt
    14641 gtattaggta gtttacgtaa ccttgcaagt attgatactt ggggaggtat acttggtttt
    14701 gcagttgctc ttgcagcggt tatgactatt tttcctttga ttacgggggt atttgctagt
    14761 gcttttgctc ctcttgctga agcagttgaa cgcaataaga aaaaagaaag tcaagctgag
    14821 caaggagcac tggataaaaa acgttggttt attgcagttg atgacggtgt tggttttggt
    14881 gaaccagcaa ctattatcgc aggtctcatt ttagtaccaa taatggttgt tatttccttg
    14941 attttacctg gaaatgaagc tttaccagtc gttgacctta ttgctatccc ttttatgatt
    15001 gaagctatga tagctgtgtc aaaaggaaat attcttaaag ctattttgaa tggaatcata
    15061 tggttttcac taggtcttta tgctgctagt gccctaggtc caatctacac agaagctgtt
    15121 aagcattttg gaactgcttt accagcaggg gtaactttga ttatgagctt taatctccta
    15181 gctcatcctt tgacagctct agtcttcttt gcttggatat ctgggaaccc actctatatt
    15241 ggtgtgacag ttgttatcta tcttgctttc ttgttcttgc ttcgtactca tcgtgaatct
    15301 atttacgcat accttaaaac tatggctgat aagaatagcg gatatgaagg tagttgtgaa
    15361 aaaatcttta ttgctgaaga ttaaaaatct gttggaggaa tactcatgaa aaaaattgat
    15421 ggacatgctc atattgttga tacaattgca ggatttaatg gtaaaggacg tcttaatgcc
    15481 cttggaaacg gttatgctat ttgggatgat ggtgatttaa ttaagctcat cccagacgaa
    15541 tacggtgaca aagcatttac agctgaagct tttttgaagt atatggatgc taatggtgta
    15601 gataaagcag tcattttgca aggtcatctt aatggttatc aaaattatta tacgcattta
    15661 gctattaaac gttatccaga acgttttaca ggcgcattct ctgttgatcc atttgcagat
    15721 aacgcaatgc aaattgtaaa acgtcatgtt gaagtccttg gttttcgagc tattaaattt
    15781 gaaattagtc aaggaggggg tatccatgga taccgtggac aaaaaacgcc tttccgtctt
    15841 gatacagacc cacatgtgag tcgtattttg acttatctgt tagactatcc aggatttgta
    15901 gttacagttg attatggcaa ttgggatcaa attagtcacc aacctgatgc tattgctaat
    15961 ttagctcgcc tttatacgag tctcgatttt gttgtttgtc acctctcttt tccacatgtt
    16021 gagcattcaa atcgattgcg tgctgaactt aatatgtgga aaaacttcaa caatatctat
    16081 actgatattt cagctattca agatattgat gccccagata gttttccttt tccaaaatca
    16141 gaacaaaatg tacgtattgc taaagaagtt cttggggcta agcgtatcat ttggggaact
    16201 gattctccat ggtcagcgac cttcaatact tacgaagaat tagctacttg gctagagaat
    16261 gttgatattt tcagtcaaga ggaactagaa gatgttatgt acaataatgc tgagcgtgtt
    16321 tactttaaag aagaacatgt aaaggctaac agagcaggta tagatgatac aaccaaagag
    16381 ctcaatctct attagaagag ttttcaaggt acaagagttt cttgtacctc ctttttaaat
    16441 caacaatgag gagacaaatt atgacttatt accttagtat tgactacggc ggtacaaata
    16501 ccaaggcgct tatttttgac aaattaggac accaaatcgc tgtttcgagt tttgaaactt
    16561 taaaaaatga gactcaatct ggtcatcgtc aagtaaacct tgttaaaacg tggaatgcta
    16621 taacttctgc tattagagag gttattcaaa tctcaaaact cagccctgag cagattagtg
    16681 cagtagcatg tattggacat gggaaaggtc tttatctgct agataataag ttggagccac
    16741 ttgaacaagg aattttgtct acagataatc gtgccaaaga tttggcgcaa tatttcgaat
    16801 ctaaacttga taatatttgg gagttgactc ggcaacatat tttcccttca caaagtccag
    16861 ttattttacg ttggcttaaa gattatcagc ccgaaaccta taaatcaata ggggcggtcc
    16921 tttctgcaaa ggactttatt cggtataagc ttacaggaaa agtacagcaa gaatatggtg
    16981 acgcttcagg taatcattgg ataaatttcc aaacaggaac ttatgatcca gctattttag
    17041 atttttttgg cattagagag atagaaaact cacttcctga acttatagat agtgcagatt
    17101 tagttcctgg gggaattagt tctcaagcag caaaagagac tggtcttgta gaagggaccc
    17161 ccgttgttgg agggctcttt gacatcgatg cttgtgctct tggatcaggt gttttagagt
    17221 cagatacttt tagtgttatt tcgggaactt ggaatattaa tacatatcca agtttaaaac
    17281 cagcaaagca agatagtggt cttatgactt cctattttcc agatcgtcgt tatctcttag
    17341 aggcaagtag ccctacttct gcagggaatc ttaattttat gttaaaaatg ctcatgcatc
    17401 aagaaattga taacgctaaa tctagtggag gttctatcta tgataattta gaagaatttc
    17461 tcactcatac tgatgctaca catcatggac ttattttctt tccgtttctt tacggtagta
    17521 acacatcaca agatgctagc gcttgctttt ttgggctaac aactaaatcg acgaaatctc
    17581 agatgatacg tgcggtatat gaaggtattg cgtttgcaca taagcagcat atcactgatt
    17641 taataaaaag taggggcagt gtgccaaaaa taattcgttt ctctggcgga gctaccaact
    17701 caccagcatg gatgcaaatg ttttctgata tcttaaactt tcctattgaa acagtagaag
    17761 gcacagaatt aggagggtta ggaggagcta ttttagcacg tcatgcttta gataagattt
    17821 cgttaaagga agcagtccaa gatatggttc gtgtaaaagc tatttataaa cctcaattat
    17881 ccgaagtaaa ggggtacaaa aaaaaatatc acgcttacca aaaattatta gaaacactgg
    17941 atcttatttg gtcggaactc ggtcatctga ataagtaggg atatcaaagg agttgttatg
    18001 aaacaagaag atattattat caatagtatt gtattcaaaa aacagttaga caaaggtttt
    18061 acacaagttc agctcattaa gttagtaaag gatttaggat tttcacgatt tgagatacgt
    18121 caagaattgc ttcaagatcc agatagagag ctacccgctt taaaagcaga ggctgacttt
    18181 tatgatatca atctttacta tagtgctaat gaagacctta tcaaaggagg gaaagttaat
    18241 ccttatctta ataaaggcct taaagaagca tcacagttag gagctccttt cattaagcta
    18301 aatgttggac aaaccagaaa cttaagtaaa gaagagttag aacctcttaa agagatattg
    18361 aaaagccaaa ctattggtat aaaggtggaa aataatcaag accctaaagc agctacagtg
    18421 gagaattgcc aatattttat gactctagtg aaagaattac aaataccaat aagttttgtt
    18481 tttgatactg ctaattgggc ctttatcaac caagacctat accaagcggt aaataactta
    18541 gcttgtgata ccacctacct tcactgtaaa aattttatac aggtagctgg aaagccccac
    18601 ctctcgaaga gcctcttcga aggagagatt aatctgactg atttgttaaa gagtttctct
    18661 aattgtgagt atctagctct agaatacccc acagagttgg agatattaaa aagggatgtt
    18721 caacgtttaa tctctatcag taatagtcaa aacttttaat gaaaaggctt taaaaagatg
    18781 ttataataag tctgtatgaa ttcgagtaga aaagaggtat attcttgatt attctcgata
    18841 aaaaaagtta tgacctcctc ttttacctat tgaaattaga ggaacctgaa acagttatgg
    18901 caattgccaa cgcacttaat cagtctagac gtaaagtgta ttatcactta gagaagataa
    18961 acgatgcact gcctagcgat gtgcctcaga ttgttagtta tccacgtgta ggaatcttgc
    19021 taacagaaaa acaaaaagca gcctgtcgtc ttttattaga tgaagtgact gattatagtt
    19081 acgtcatgaa aagtagtgag aggttgcagc tgtctttagt atctatcgta gtagctaagg
    19141 accgcgtaac gattgatagg ttgatgcaac taaacgatgt ttctcgaaat actatcttaa
    19201 acgatttaaa cgaattaaga agtgagttag cagagaaaga atataattta cagttacaat
    19261 caacaaaatg tcgtggttat tttttagatg gtcacccatt gtccattatc cagtacttat
    19321 ataagctctt agatgatatc taccataatg gaagtagtag ttttatagac ctttttaatc
    19381 ataaactgtc tcaagctttt ggtgccagca cttatttttc taaagaggtt cttgattatt
    19441 ttcatcatta tctcttcatt tctcaacgaa gtctaggtaa gaaaatcaac agtcaagatg
    19501 gtcagtttat gattcagatt ttgcctttta tactaatggc ttatcgtaag atgcgattaa
    19561 gtcctgaagt acagacctct cttaatagtg attttagctt ggtttggcaa cgtaaggaat
    19621 atgagattgc taaagagttg gctgatgagc tggaagaaaa ttttcagtta tcactggatg
    19681 agattgaagt gggactagta gccatgctta tgcttagttt ccgcaaggac cgtgacaatc
    19741 atttagagag ccaggattat gatgatatgc gagctactct aaccagtttt ttgaaagaat
    19801 tggaagaacg atatcacctt cactttgttc ataaaaagga cttactaaga caacttctta
    19861 ctcactgcaa ggcactctta tatcgtaaac gttatggtat tttttctgtt aatcctttaa
    19921 cagagcatat taaagacaaa tatgaagaac tttttgccat aacctcgtct tctgtaaagc
    19981 ttttagagaa agcttggcaa atcaaattga ccgatgatga tgtagcatat ctaacgattc
    20041 atttaggagg ggaacttcgt aatagtcaac aatctcctaa taaacttaag ttagttattg
    20101 tatctgatga aggaatagcg attcagaaac ttcttttaaa gcaatgtcaa cgctacttaa
    20161 caaatagtga tatagaagct gtttttacaa ccgaacagta tcaaagtgtg agtgatctta
    20221 tgcatgtaga tatggttgtc tctactagtg atgctttaga atctcgtttt ccgatgttag
    20281 tagttcaccc tgttttgaca gatgatgata ttattcgctt gattcgcttt tctaaaaaag
    20341 gtaactgtgc aaatagtaat caatttacca atgaacttga aaaaacaatt gctcaatatg
    20401 tcaaggaaga tagtgaacgc tacgtgctga aatctaagat tgagaaactt attcatcaag
    20461 aattgctcca agacgtcctt ccccttcaaa gtacagtttg ttaaaatata attttatggt
    20521 agcctctcag cttttctgaa gacagaaaaa gaagctgggg cttttttggt gcttttaaac
    20581 aatgtgctag atatttaatc gcaatgagtt cctctatgac atgataatta aagttgagaa
    20641 agctagtact tttaatggga gtacttttta tttgtgctta ttttaattaa aattatgagt
    20701 ctaatttgga acagacagcc cagttttgaa cacataaatg tgtgacactt ggtctttttt
    20761 tcaaaagtat ttgaacttat actagtagat gtaagaaaca attaatcagg aggaaataaa
    20821 atgcctaacg ttaaagatat tactagagaa tcatggattt tatcaacatt cccagaatgg
    20881 ggtacatggc taaacgaaga aattgaagaa gaagttgttg ctgagggcaa ctttgcaatg
    20941 tggtggttag gaaactgtgg tgtttggatt aaaacaccag gtggtgctaa tgttgttatg
    21001 gacctatggt caaaccgcgg taaatcaaca aaaaaagtaa aagatatggt tcgtggacac
    21061 caaatggcta atatggcagg tgttcgtaaa ctccaaccaa acttacgcgc acaaccaatg
    21121 gttattgatc catttgctat taatgaattg gattactatc ttgtttcaca tttccacagt
    21181 gaccacattg atatcaacac tgcggcagcc attattaata atcctaactt agaccatgtt
    21241 aaatttgttg ggccatatga atgtggtgaa atttggaaaa aatggggagt tccagaagaa
    21301 cgaatcatcg tgattaaacc aggtgaatca tttgagttta aagatatcaa agttactgct
    21361 gttgaatcat ttgaccgtac ttgcttggtg acacttccag tagatggtgc agaagaacat
    21421 gatggagaac ttgctggttt agccgtgact gatgaagaaa tggctcgtaa agcagttaac
    21481 tatatttttg aaacaccagg tggaacaatc tatcatggag ctgactcaca tttttcaaat
    21541 tattttgcta aacatggtaa agactacaag attgacgtag ctattaacaa ctatggtgat
    21601 aacccagttg gtattcagga taaaatgact tcaatcgatc ttcttcgtat ggcagaaaat
    21661 ctccgtgcca aagtcattat tcctgtacac tacgatatct ggtctaactt catggcttct
    21721 acagacgaaa tcttacaatt gtggaaaatg cgtaaagaac gccttcaata tgacttccat
    21781 ccatttattt gggaagttgg aggtaaatac acttatccac aagataaaga tagaattgaa
    21841 taccatcacc cacgtggttt tgatgattgc ttcgaacaag aatcaaatat tcaatttaaa
    21901 gcacttctat aatggctaat gagagactgt atgtcgcatg atatgcagtt tttcaatttt
    21961 aaaatatgtt actaaaatca caattaagat taggagaaat aatgtcagaa tttgatacaa
    22021 aatcatattt agaaaaactt gatgcatggt ggagagcagc taattatatt tctgcagcac
    22081 aaatgtatct aaaggataac cctctcttga gacgagagct ggttgaaaac gacttaaagg
    22141 ttcatccaat tggtcactgg ggcactgtac ctggacaaaa ttttatctat gctcacttaa
    22201 atcgcgctat taataaatac gatttagaca tgttctatat tgaaggtcca ggtcatggtg
    22261 gtcaagttat ggtatctaat tcatatttag atggttcata tactgaatta aacccaaata
    22321 ttgagcaaac agaggatggc tttaagcagt tatgtaaaat cttctctttc ccatgtggaa
    22381 ttgcatccca tgctgcacca gaaacaccag ggtcaattca tgaaggcggt gaacttggtt
    22441 acgcactttc tcatgcgaca ggtgccatcc tagacaatcc agatgttatt gctgcgactg
    22501 ttattggtga tggtgaaggt gaaacaggac cacttatggc tggttggcta tctaatacct
    22561 ttatcaatcc agtaaatgat ggtgctgttc taccaatctt ctatttaaat ggtggtaaaa
    22621 tccataaccc aactatcttt gaacgtaaga cagatgaaga attatctcag ttttttgaag
    22681 gattaggttg gaaacctatt tttgcagatg ttgttgaact ctctgaggat catgcggctg
    22741 ctcatgcttt gtttgcagaa aaattagatc aagctattca agagattaaa accattcaat
    22801 cagaagcacg acaaaaacca gcagaagaag ctatccaagc aaaattccct gtcttagttg
    22861 cacgtattcc taagggttgg actggtccaa aagcttggga aggaacacca attgaaggtg
    22921 gcttccgcgc tcaccaagta ccaattccag tagatgccca ccatatggaa catgttgatt
    22981 ctcttttgtc atggcttcaa tcataccgtc cagaagaatt atttgatgaa aatggtaaaa
    23041 tcgttgatga gattgctgct atttcaccaa aaggtgatcg tcgcatgtcc atgaatccaa
    23101 taaccaatgc tggtattgtt aaagcaatgg atacagcaga ttggaagaaa tttgctcttg
    23161 atattaatgt tccaggtcaa attatggcac aagatatgat tgaatttgga aaatatgcag
    23221 cagatttggt agatgctaat ccagataatt tccgtatttt tggtccagat gaaacgaaat
    23281 caaatcgtct tcaagaagtg tttacacgta ctagccgtca atggcttggt cgtcgtaaac
    23341 cagattatga cgaagcttta agtccagctg gacgtgttat tgattcccaa ttgtctgaac
    23401 accaagcaga aggtttctta gaaggttatg ttttaactgg tcgtcacggc ttctttgctt
    23461 catacgaatc attccttcgc gttgtagatt caatggtaac acaacatttc aaatggttac
    23521 gtaaatcaaa aacacacaca acatggcgta aaaattatcc agcgcttaac ctaattgcag
    23581 cttcaactgt tttccaacaa gatcataatg gttatactca ccaagatcca ggtattttaa
    23641 ctcatttagc tgaaaaaaca cctgaataca ttcgtgaata tttaccagca gatactaact
    23701 cacttctagc tgttatggat aaagcgttta aagctgaaga taaaattaat ttaattgtga
    23761 catctaagca ccctcgtcca caattttact ctattgctga agcagaagag ttagtggcag
    23821 aaggatataa ggtgattgac tgggcttcaa atgtgtcgct taatcaagag ccagatgttg
    23881 tctttgctgc ggcgggaaca gaacctaact tagaagcttt ggcagctatt agcattcttc
    23941 ataaggcttt cccagaactt aagattagat ttgttaatgt attagacatc ctaaaacttc
    24001 gtcatccttc acaagatgct cgcggtttat cagatgaaga gtttaacaaa gtctttacca
    24061 cagataaacc tgttattttt gctttccatg gttatgaaga tatgattcga gatattttct
    24121 ttagtcgtca caatcataat ttgcatacgc atggttaccg tgaaaatggt gacatcacaa
    24181 caccatttga tatgcgcgtg atgtcagaat tggaccgatt ccatctggca caggatgcag
    24241 ctcttgcttc tttaggaaat aaagcacaag ccttcagtga tgaaatgaat caaatggttg
    24301 cttatcacaa agactatatt cgtgaacacg gagacgatat tccagaagta caaaattgga
    24361 aatgggaaaa tatcaagtag tcactttttt caggcctata cttacaaaca aaaacacttt
    24421 tagatttttt ctaagggtgt tttgttattt tatagaagaa tgaccgcttt ttcaaaagta
    24481 ttaagtggtc acttttaaag ttgtaatatt aatactaata agtctttaaa taattcaata
    24541 atgccataac aataatggtg tgggctaatt aaaatatctc taagcaaagg tgttgacata
    24601 aagagaccat acatgatatt atagttatgt tctgtttcgg cagtttattt ttttgagtaa
    24661 aaaagtgact acccttttac gataacttaa cgtcacttta tagataagac acataacctt
    24721 aaaaataaga tacgaaattt ttagtaggag ataagaatga caaatatttt agaagtgaag
    24781 aatttaacca aaatttttgg gaaaaagcaa aaagctgctc ttgaaatggt taagcaagga
    24841 aaaagtaaga cagaaattct tgaaaaaaca ggagcgactg taggtgtcta tgatgcgagt
    24901 tttgaaataa aagaaggcga aattttcgtt atcatgggac tttctggttc aggaaaatca
    24961 actttagttc ggatgttaaa ccgtttgatt gacccatcat caggaaatat ctatttagat
    25021 ggtaaagata ttgcaaagat gaatgtagaa gatttacgta acattcgtcg tcatgatatc
    25081 aacatggttt tccaaaattt tggtcttttc ccacatcgta caattttaga aaatacagag
    25141 tttgggttag agatgcgtgg tgtttctaag gaagaacgta caacgttagc tgaaaaagct
    25201 ttggacaatg caggtctatt accgtttaaa gatcagtacc ccagtcaatt atcaggagga
    25261 atgcaacagc gtgttggttt agcacgtgcc cttgctaaca gccctaaaat ccttctgatg
    25321 gatgaggcct tctcagcttt agacccatta attcgtcgtg aaatgcaaga tgagcttctt
    25381 gatcttcaag acacgaataa acaaactatt atttttatca gccatgattt gaatgaagcg
    25441 ttacgtattg gtgatcgtat tgctctgatg aaagatggag aaatcatgca gattggtaca
    25501 ggagaagaga ttttgactaa tccggctaat gattttgttc gtgagtttgt tgaagacgtt
    25561 gatcgctcaa aagtgttaac tgctcaaaat attatgataa aacctttaac aactgtttta
    25621 gaaattgatg gcccacaagt tgctttaaca agaatgcatc gtgaggaagt ctcaatgtta
    25681 atggcaacga atcgtcgtcg ccaacttctg ggatccctaa ctgcagatgc tgcaattgaa
    25741 gctcgtaaga aagatttgcc gctttctgaa gtgattgata aagatgttgt aacggtctca
    25801 aaagatacag ttattacaga tatcatgcct ttaatttatg attcatctgc tccgatagcc
    25861 gttactgatg ataatgatag actcctaggg gttatcatta gaggacgtgt gatcgaagca
    25921 cttgctaacg ttcaagatga aacagtagta gaatcgccaa aagaaacggt agaagcttaa
    25981 tagagaggac aaatcatttt ggaaaattta ttacaacata agttaccggt cgcaccattt
    26041 gtagagtcaa cgaccaattg gattacgaaa actttttctg gtttatttga ctttatccag
    26101 acaatcggta atgcattgat ggattggatg acaaaaacat tactattcat taatccttta
    26161 ctttttattg tactaattac aattgctgta tttttcttag ctaagaaaaa atggcaatta
    26221 ccgacattta ctttcattgg tttgctattt atctataacc aagggctgtg ggaacagttg
    26281 attaatactt ttaacttggt tttagtagca agtttaattt ctattattat tggtgtacca
    26341 ctaggtattt ggatggcaaa aagtgataaa gttaaacaag ttgttaatcc aattcttgac
    26401 tttatgcaaa ctatgccggc attcgtttac cttattcctg cagtagcctt ctttggtatc
    26461 ggtatggtac caggtgtctt tgcctctgtt gtatttgctc tgcctccaac agtgcgcttt
    26521 actaacttgg ctattcgtga aatcccactt gaattaatag aagcatctga ttcatttggt
    26581 tcaacagtta agcaaaaatt attcaaagtt gaactaccac ttgctaagaa cactattatg
    26641 gcaggaatca atcaaactat gatgttggct ctttctatgg tggttacagg atctatgatc
    26701 ggtgcacctg gacttggtcg tgaggtgtta tcggcgcttc aacatgctga tattggtaca
    26761 ggatttgtta gtggtctatc gctagttatt ttagcaatcg tgttagaccg tgttagtcaa
    26821 ttctttaatt ccaaaccggg tgagaagcaa gctaaaacat caaaagttaa aaaatgggta
    26881 ggtttagggg ccctggctct cttcattcta gcagctcttg gtcgcattgt tgtcaatatg
    26941 acttcaggaa atgaagccaa aggtcaaaaa gtgaagatag cttatgtcca atgggattca
    27001 gaagttgctt caaccaacgt tattgctgaa gttttaaaat ctaaaggata tgatgttgaa
    27061 ttgacaccac ttgacaatgc ggtcatgtgg caaacagtcg ccaatgggaa tgctgatttt
    27121 acaacaagtg cttggttacc aaagacccac gggcaatatt ttaataaata caaaaatagt
    27181 ttagatgact taggtcctca cgttgagaac gtcaaaatag gtttagttgt tcctaaatac
    27241 atgaacgtaa atagtattga agaattatca aatcaagcag ataaacaaat tacaggtatc
    27301 gaaccaggtg caggtattat gaagtctgct aaacaatccc ttaaagatta tccaaacctt
    27361 tcaagttgga agttgttatc agcatcaaca ggagctatga caacaacctt agggaaggct
    27421 atcaaaaaca aagatcaagt agtcattaca ggttggtcac ctcactggat gtttgctaag
    27481 tatgatttaa aataccttaa agatcctaag aaatcatttg gtggtgaaga acatatcaat
    27541 acaatagcac gcaagaattt gaaaaaagat atgccaaaag tatataaaat tatcgataag
    27601 ttcaagtgga ctaaagaaga tatggaatct atcatgttag atatggataa aggtatggag
    27661 cctgctaaag cagctcaaaa atggattaaa aaccacaaaa aagaagtttc agagtggact
    27721 aaatagtata gaaaaaaaca cgtctctttt agagcgtgtt ttttaatgtg acttaagtcg
    27781 ttttagctat ttaatataag ttttatatta ttttaataca tcaaaagtga gagaatgaaa
    27841 gaaatggcag gttataaagt attactaagc taaattctca aagtaagttc cacctctagt
    27901 agaaatggaa tttagtctga taaaaaccaa taaaaaccaa cgaaaatccg tctcagacta
    27961 agtt
//
LOCUS       DATRMA010000021        27715 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.021, whole genome shotgun sequence.
ACCESSION   DATRMA010000021 DATRMA010000000
VERSION     DATRMA010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 27715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27715
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.021"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            complement(397..1992)
                     /gene="cls"
                     /locus_tag="VBC28_000848"
     CDS             complement(397..1992)
                     /gene="cls"
                     /locus_tag="VBC28_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEO6814849.1"
                     /translation="MIWRGEKLISNKVKIVRLLNKSKKSLLRGIFSRTTVIAILLILQ
                     LLFLLASYSWLEQYRVWLATVEHILTIGAVLYLVNSEMDALSRVTWLILVMIAPLLGA
                     MFLMYTKFDWGYRGLKQRLETLIDESQIYLEDDPETLNQLKSSTSTTYHLVQYFEKAH
                     GNFPVYRNTDVTFLPTGEAFFEKMKEELLKAKKYIFLEFFIIDEGIMWGEILSILEQK
                     VEEGVEVRILYDGMIEITKLSFDYTKRLEKIGIKAKAFSPISPFISTYYNYRDHRKIV
                     VIDGVVGMTGGVNLADEYINHIELFGHWKDSGIMLKGKAVDSFLLLFLQMWSITEKKM
                     LVAPYLGVHDDLVENEGYVIPYGDSPLDTDKVGENVYIDILNHAREYVYIMTPYLILD
                     SELEHAIQFAAERGVDVRIIMPGIPDKPIPYALAKTYYQALTKSGVKIYEYTLGFVHS
                     KIFLSDNTKAVVGTINLDYRSLYHHFECAVYLYKVDAIQDIYRDYMDTLNKSRLVSLK
                     DINNIPKFQKVIGIVTKTIAPLL"
     gene            complement(2111..3781)
                     /locus_tag="VBC28_000849"
     CDS             complement(2111..3781)
                     /locus_tag="VBC28_000849"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEO6814850.1"
                     /translation="MKTDIEIAQSVALKPIAEIVEQVGIGFDDIELYGKYKAKLSFDK
                     IEAVRSQKVGKLILVTAINPTPAGEGKSTMSIGLADALNKIGKKTMIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELDIDQRR
                     VIWKRVVDLNDRALRQVIVGLGSPVNGIPREDGFDITVASEIMAILCLATDLSDLKKR
                     LSNIVVAYSRDRKPIYVKDLKIEGALTLILKDAIKPNLVQTIYGTPALVHGGPFANIA
                     HGCNSVLATSTALRLADYVVTEAGFGADLGAEKFLDIKTPNLPTSPDAIVIVATLRAL
                     KMHGGVSKEDLSQENVKAVKRGFTNLERHVNNMRQYGVPVVVAINQFTADTESEIATL
                     KTLCSNIDVAVELASVWEDGADGGLELAQTVANVIETQSSNYKRLYNDEDTIEEKIKK
                     IVTKIYGGNKVHFGPKAQIQLKEFSDNGWDKMPICMAKTQYSFSDNPNLLGAPTDFDI
                     TVREFVPKTGAGFIVALTGDVLTMPGLPKKPAALNMDVLEDGTAIGLF"
     gene            complement(3870..4889)
                     /locus_tag="VBC28_000850"
     CDS             complement(3870..4889)
                     /locus_tag="VBC28_000850"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HEO6814851.1"
                     /translation="MYLIEPIRNGKRITDGAIALAMQVYILQNVFLDDDILFPYYCDP
                     KVEIGKFQNAVIETNQEYLKEHDIPVVRRDTGGGAVYVDSGAVNICYLMKDHGQFGDF
                     KRAYEPAIKALKTLGASSVEMRGRNDLVIDGKKVSGAAMTIVNGRIYGGYSLLLDVDF
                     DAMEKVLNPNRKKIESKGIKSVRSRVGDIRSHLSEDYRHITTDQFKDLMVCQLLHIDH
                     IDQAKRYHLTEKDWAAIDALADEKYKNWDWNYGNSPQYSYHRDARFPSGTYDFHLEIE
                     KGIITNCRIYGDFFSSKDISDIENLLIGCPMKEELVLEKLSTVSLEDYFGQTSPEEIK
                     AVLFP"
     gene            complement(4916..5794)
                     /locus_tag="VBC28_000851"
     CDS             complement(4916..5794)
                     /locus_tag="VBC28_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacetylase"
                     /protein_id="HEO6814852.1"
                     /translation="MTVWKTLEKTNHSQSEILSQLIEESDAIVVGIGAGMSAADGFTY
                     IGPRFEEAFPDFIAKYQLLDMLQASLYDFEDWEEYWAFQSRFVALNYLDQPVGQAYLD
                     LKDILAKKEYHIITTNADNAFAVADYNLEKVFHIQGEYGLWQCSQHCHQQTYRNDQAI
                     RQMIAQQKDMKIPSNLIPKCPKCDQPFEINKRNEEKGMVEDADFHAQRQRYENFLSQH
                     QNDKVLYLEIGVGHTTPQFIKHPFWRFVSLNENSLFVTLNHKHYRIPQKIRSRSVQLT
                     QHIAELIAEAKKEIIG"
     gene            complement(5763..6581)
                     /locus_tag="VBC28_000852"
     CDS             complement(5763..6581)
                     /locus_tag="VBC28_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-ADP-ribose hydrolase"
                     /protein_id="HEO6814853.1"
                     /translation="MPNQKQLLLAMIEYLRSEKLTDVDDLRTTDLQTVWRALVNQQDP
                     QNISQEYLSLEDRYLSHWWNTQKVKTIDVCHQTVYSNVFTYHGDICLLQVDAIVNAAN
                     GKLLGCFIPNHHCIDNHIHTFAGSRLRLACHQLMTQQGRMEAVGQAKLTESYHLPCKY
                     VIHTVGPYVKVDQKPSRIREDLLKSSYKSCLQLAVRANLKTIVFPCISTGEFGFPNQR
                     AAELAVQAILEWQRENQHKLYIIFNTFTPKDQEIYQKLLLKEEIDDSVEDSRKN"
     gene            complement(6574..6906)
                     /locus_tag="VBC28_000853"
     CDS             complement(6574..6906)
                     /locus_tag="VBC28_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000717324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HEO6814854.1"
                     /translation="MKKIANYLLIEKNEERYTISLTPELQDDLGTVGYVEFTDDTNLE
                     VDDVILNIEASKTVMAILSPLTGKVVKVNTAASQEPTLLNSEKADENWLVVLTDVDYA
                     AFEALENA"
     gene            complement(6935..7921)
                     /locus_tag="VBC28_000854"
     CDS             complement(6935..7921)
                     /locus_tag="VBC28_000854"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MsnO8 family LLM class oxidoreductase"
                     /protein_id="HEO6814855.1"
                     /translation="MKLSVLDYGLIDYGKTASDAIQETILLSQEAERLGYHQFWVAEH
                     HGVKAFSISNPELMIMHLANQTKSIKIGSGGIMPLHYSSFKLAETLKTLETCHPNRVS
                     IGLGNSLGTVKVSNALRSLHKAHDYEEVLEELKSWLIDESSSKEPLVQPTLSSFPDLY
                     VLGSGQKSAYLAAKLGLGFTFGVFPFMDKDPLTEAKKLSSLYYHQFEEYYPNKSPNLM
                     VAAFVVIADTSEEAENIAKTLDIWMLGNKDFNEFATFPTIEEANHYQLTPEQKAKIKS
                     NRHRMIVGDPKQVKDSLDALVNASQAEELLLIPLVPGLDQRIKSLKLLSQLY"
     gene            complement(7918..9117)
                     /locus_tag="VBC28_000855"
     CDS             complement(7918..9117)
                     /locus_tag="VBC28_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HEO6814856.1"
                     /translation="MNNVQGNLFRPLILPNGLSLENRFVLSPMVTNSSTSEGFVTDDD
                     IAYAVRRAKSAPLQITGAAYVTEYGQLFEYGFSVSKDEDIPGLTKLAKAMKSKGAKAV
                     LQLTHAGRFSSHTLARHGYVYGPSPMQLQSPYPHQVKELTHKDILMIIDEYVQATRRA
                     IQAGFDGVEISSAQRLLIQTFFSTFSNQRKDEYGPQTLTNRCRLGLEVFKAVQKVIRE
                     EAESDFILGFRATPEETRGSQIGYSIEEFMEFLEKILAIAQVDYLAIASWGHDVFRNT
                     IRSEGVYKGQLVNQVIFEHFGDRVPIMATGGINSASKVFEALQHAHMVGASTPLVVDP
                     EFLQKIKAKRSDQINLRIKVSDLEGLAIPKASFKDIVPLMDYGESLPKEAREVFRELR
                     SNYREER"
     gene            complement(9110..9946)
                     /locus_tag="VBC28_000856"
     CDS             complement(9110..9946)
                     /locus_tag="VBC28_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HEO6814857.1"
                     /translation="MEWQDLAQLPVSIFKDYVTDAQDAEKPFIWTEVFLREINRSNQE
                     IILHIWPMTKTVILGMLDRELPHLELAKKEIISRGYEPVVRNFGGLAVVADEGILNFS
                     LIIPDVFERKLSISDGYLIMVDFIRSIFSDFYQPIEHFEVETSYCPGKFDLSINGKKF
                     AGLAQRRIKNGIAVSIYLSVCGDQKERSRMISDFYKIGLGDTGSPIAYPNVDPEIMAN
                     LSDLLDCPMTVEDVIDRMLISLKQVGFNDRLLMIRPDLVAEFDRFQAKSMANKGTVSR
                     DE"
     gene            10085..10786
                     /gene="coaB"
                     /locus_tag="VBC28_000857"
     CDS             10085..10786
                     /gene="coaB"
                     /locus_tag="VBC28_000857"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HEO6814858.1"
                     /translation="MYNMAMKILITSGGTTEKIDTVRSITNHATGALGKIIAEKYLRE
                     GHQVTLVTTKNAIKPESATNLSIFEIEDVDSLIKTLKPLVKEHDILIHSMAVSDYTPV
                     YMADFEKVKSSDDLDTFLRKDNHEGKISSESEYQVLFLKKTPKVISLVKKWNPQIILV
                     GFKLLVNVTKENLFKVARHSLIKNKATFILANDLIDITSKHHIAYLLDHDNVYKATTK
                     EDIAQLIYEKVKKYD"
     gene            10779..11321
                     /gene="coaC"
                     /locus_tag="VBC28_000858"
     CDS             10779..11321
                     /gene="coaC"
                     /locus_tag="VBC28_000858"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HEO6814859.1"
                     /translation="MIKRITLAVTGSISAYKAADLTSQLTKIGYDVHIIMTQAATEFI
                     TPLTLQVLSKNPIHLDVMDEHNPKIINHIELAKRTDLFIVAPASANTIAHLAYGFADN
                     IVTSVALAMPDETPKLIAPAMNTKMYHNTITQRNIDILKKIGYQEIEPRISLLACGDT
                     GQGALADISTILKCIQEVAN"
     gene            11367..11939
                     /locus_tag="VBC28_000859"
     CDS             11367..11939
                     /locus_tag="VBC28_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEO6814860.1"
                     /translation="MKKKNKSSNIAIIAIFFAIMLVIHFLSSFIFSFWLVPIKPTLMH
                     IPVIIASIAYGPRIGATLGALMGGISVANSSIVLLPTSYLFSPFVENGNFYSLIIALV
                     PRILIGIIPYFVYKLLHNRFGLAISGAIGSLTNTVFVLSGIFIFFSSTYNGNIKLMLA
                     GIISSNSLAEMVIAVIIVYLTVPRILNIKH"
     gene            12049..13767
                     /locus_tag="VBC28_000860"
     CDS             12049..13767
                     /locus_tag="VBC28_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HEO6814861.1"
                     /translation="MTYTENFQKWLDFEQLPDYLRQELLSMDEKTKEDAFYTNLEFGT
                     AGMRGYIGAGTNRINIYVVRQATEGLAKLIETKGEEAKKRGVAIAYDSRHFSPEFAFE
                     SAQVLAQHGIKSYVFEALRPTPELSFAVRHLNAYAGIMVTASHNPAPFNGYKVYGQDG
                     GQLPPADADALTDFIRAIENPFAVELADLDESKSAGLIQVIGEDVDIEYLREVKDVNI
                     NQDLINNFGKDMKIVYTPLHGTGEMLTRRALAQAGFESVVVVESQAKADPDFSTVKSP
                     NPESQAAFALAEELGREVDADVLVATDPDADRLGVEIRQPDGSYKNLSGNQIGAIIAK
                     YILEAHKTAGTLPENAALAKSIVSTELVTKIAESYGATMFNVLTGFKFIAEKIQEFEE
                     KHNHTYMFGFEESFGYLIKPFVRDKDAIQAVLLVAEIAAYYRSRGLTLADGIDEIYKE
                     YGYFAEKTISVTLSGVDGAAEIKKIMDKFRENGPKQFNNTDIVLLEDFQKQTATKNDG
                     IISNLTTPPSNVLKYTLADDSWIAVRPSGTEPKIKFYIATVGNDLADAETKIANIEKE
                     ITTFVN"
     gene            complement(13791..14030)
                     /locus_tag="VBC28_000861"
     CDS             complement(13791..14030)
                     /locus_tag="VBC28_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6814862.1"
                     /translation="MKKRLQLLLLIFISAVSWFISNQLATKNTVLSVLIGLVAVIGIT
                     WLIVSLLYYLIVKLIAVIFHIIENNRTSLDRYKKS"
     gene            complement(14044..15777)
                     /locus_tag="VBC28_000862"
     CDS             complement(14044..15777)
                     /locus_tag="VBC28_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6814863.1"
                     /translation="MKASHPKKIQKKLVQDLLSKKSLVGMALLGTVVQVCLTVYLPVL
                     IGQAVDVVLSPHSMILLLPIMWKMIAVILANAIIQWINPLLYNRLIFHYVASLRKAVM
                     EKLNLLPIAYLDKRGIGDLISRVTTDTEQLSNGLLMVFNQFFVGLLTILVTIFSMAKI
                     DLLMLFLVLFLTPLSLFLARFIAKKSYHLYQNQTASRGRQTQFIEEMVSQESLIQAFS
                     AQEESSNHFRTINQEYANFSQSAIFYSSTVNPSTRFINSLIYGFLAGIGALRIMSGAF
                     SVGQLITFLNYVNQYTKPFNDISSVLSEMQSALACAERLYSILEESSPNITGTEKLDS
                     STVKGQIDFKNVVFGYNKSKPLLNGINLHIPAGAKVAIVGPTGAGKSTLINLLMRFYE
                     VDGGNILLDGKPITDYEPSQLRQEIGMVLQETWLKSATIHDNIAYANPKASRDEVIEA
                     AKAANADFFIKQLPNGYDTYLEDAGDSLSQGQCQLLTIARIFLKLPRILILDEATSSI
                     DTRTEVLVQEAFQMLMKGRTSFIIAHRLSTIQTADIILVMVSGEIVEVGNHSELIAQK
                     GIYYQMQNAQK"
     gene            complement(15778..17499)
                     /locus_tag="VBC28_000863"
     CDS             complement(15778..17499)
                     /locus_tag="VBC28_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000826931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6814864.1"
                     /translation="MKRLTYYFKGYIKETIFGPLFKLLEASFELLVPIVIAKMIDETI
                     PRGDRSSLLLQIGLIFFLAAVGVVVAITAQYYSSKAAVGYTRQLTEDLYQKVMSLGKK
                     DRDELGTASLITRLTADTFQIQTGLNQFLRLFLRAPIIVFGAIIMAFSISPSLTIWFL
                     VMVVTLFIIVFVMSRLLNPIYLKIRTSTDYLVKLTRQQLQGVRVIRAFNQVDRESEAF
                     NDINYHYTNLQLKAGRLSSLVTPLTFLVVNITLVVIIWRGNLNIANHLLSQGMLVALI
                     NYLLQILVELLKMTMLVTSLNQSYISAKRIIAVFERPSEIIDDKLEPKYSNKALEVQE
                     MAFSYPNSSEKALSDITFSMNVGETLGIIGGTGSGKSTLVNLLLHIYKVQEGDIDIYH
                     QGKSPDTISNWRTLVRVVPQNAQLFKGTIRSNLSLGLGKVSEEKLWTALEIAQASDFV
                     KEKDGQLDAPVESFGRNFSGGQRQRLTIARALVQDKIPFLILDDATSALDYLTEARLF
                     KAITKHFNQTNLIIVSQRINSIQNADRILLLDKGKQVGFDNHQSLLAHNKVYKSIYHS
                     QNFKEEE"
     gene            complement(17511..18113)
                     /locus_tag="VBC28_000864"
     CDS             complement(17511..18113)
                     /locus_tag="VBC28_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HEO6814865.1"
                     /translation="MFKFLKRLIALIIIIFIGYRLVIIHENVKKVLQYHDLVQNTLAE
                     NGSEANVHLVLSMIYTETKGDAIDVMQSSESISGTTNSITDSHTSIKHGVTLLSQNIS
                     QAKKAKVDVWTAVQAYNFGSSYIDYVADHGGENSIELAKNYSKNVVAPSLGNYNGDTY
                     FYYHPLALISGGKLYKNGGNIYYSREVQFNLYLIKIMELF"
     gene            complement(18115..19092)
                     /locus_tag="VBC28_000865"
     CDS             complement(18115..19092)
                     /locus_tag="VBC28_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HEO6814866.1"
                     /translation="MMDFYIKQIIIHQFSPNDTELVLSDTPLTLTPRIDDYFRKKLSK
                     VFSDEAKRGYFGEDNVFMSHLQDDLYVSSCQIAQLWKEEFVISEDQKTNDLVFIQFDK
                     DGMEHFAFLRISLKEQFAHVSENQEQPITITQNNLPSAAQTPDEALVVNKSSKQYYLI
                     EKRIKHNGSFANYFSENLLQVQPEQSVKKSIKMVEQTAQKIAENFNKDDFSFQSKMKS
                     AIYKNLEEEQELSPEKLADQLFDDNLTARLTFVDELKDAIPGPVQVSDIDHSRQIKKL
                     ENQKLSLSNGIELIVPNNVYQDAESVEFIQNPDGTYSILIKNIQDIQNK"
     gene            complement(19097..20353)
                     /locus_tag="VBC28_000866"
     CDS             complement(19097..20353)
                     /locus_tag="VBC28_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEO6814867.1"
                     /translation="MIFDKDNFKEFDQELWQAIHDEEIRQQNNIELIASENVVSKAVM
                     AAQGSVLTNKYAEGYPSHRYYGGTDCVDVVESLAIERAKTLFNAEFANVQPHSGSQAN
                     AAAYMALIEPGDTVLGMDLAAGGHLTHGASVSFSGKTYHFVSYSVDPKTEMLDYDNIL
                     KIAQETQPKLIVAGASAYSRIIDFEKFRQIADAVDAYLMVDMAHIAGLVASGHHPSPI
                     PYAHVTTTTTHKTLRGPRGGLILTNDEAIAKKINSAVFPGLQGGPLEHVIAAKAVALK
                     EALDPSFKIYGEDIIKNAQAMAKVFKEDDDFHLISDGTDNHLFLVDVTKVIENGKKAQ
                     NVLEEVNITLNKNSIPFERLSPFKTSGIRIGTPAITSRGMGVEESRRIAELMIKALKN
                     HENQDILTEVRQEIKSLTDAFPLYEN"
     gene            complement(20445..21041)
                     /locus_tag="VBC28_000867"
     CDS             complement(20445..21041)
                     /locus_tag="VBC28_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HEO6814868.1"
                     /translation="MNDLGQILEDHGAVIMPTETVYGIFAKALSEEAVNHVYELKKRP
                     RDKAMNLNICDFETILKYSKNQPTYLKQLYDAFLPGPLTIILEASQEVPHWINSGLLS
                     VGFRMPKHPVTLDMIANHGPLIGPSANISGCDSGRVFSEIQKQFNHQVLGIEDDKALT
                     GVDSTIIDLSGDRVKILRQGAITQEVLTATIPELIFEN"
     gene            complement(21034..21864)
                     /gene="prmC"
                     /locus_tag="VBC28_000868"
     CDS             complement(21034..21864)
                     /gene="prmC"
                     /locus_tag="VBC28_000868"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001104950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEO6814869.1"
                     /translation="MNYAQLIKHYGQLLEACGEEVENFIYVLKDLKQWSTTDYLLNQN
                     SSVSDTDQQLMENIFQQLKKHRSPQYITGKAYFRDLIFFVDERVLIPRPETEELVDLI
                     LSENKVEDCSVLDIGTGSGAIAISLKKERPSWDVLASDISVSALDLAKENANNCDAEV
                     TFIESDVFSNISGKFDIIVSNPPYISYNDKDEVGKNVLASEPHSALFADEEGLAIYRK
                     IIENSREYLQPRGKLYFEIGYKQGDDLRSLLKRYFPNNRCRVLKDIFGKDRMVVLDNE
                     "
     gene            complement(21864..22943)
                     /gene="prfA"
                     /locus_tag="VBC28_000869"
     CDS             complement(21864..22943)
                     /gene="prfA"
                     /locus_tag="VBC28_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEO6814870.1"
                     /translation="MNIYDQLQAVEDRYEELGELLSDPDVVSDTKRFMELSREEANTR
                     ETVTAYREYKQVIQNISDAEEMIKDASGDAELEEMAKEELKESKAAKEEYEERLKILL
                     LPKDPNDDKNIILEIRGAAGGDEAALFAGDLLTMYQKYAETQGWRFEVMESSVNGVGG
                     IKEVVAMVSGQSVYSKLKYESGAHRVQRVPVTESQGRVHTSTATVLVMPEVEEVEYEI
                     DQKDLRVDIYHASGAGGQNVNKVATAVRMVHIPTGIKVEMQEERTQQKNRDKAMKIIR
                     ARVADHFAQIAQDEQDAERKSTVGTGDRSERIRTYNFPQNRVTDHRIGLTLQKLDTIL
                     SGKMDEVIDALVMYDQTQKLEALNK"
     gene            complement(22978..23547)
                     /locus_tag="VBC28_000870"
     CDS             complement(22978..23547)
                     /locus_tag="VBC28_000870"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000068112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEO6814871.1"
                     /translation="MAQLYYKYGTMNSGKTIEILKVAHNYEEQGKPVVIMTSSLDTRD
                     EFGVVSSRIGMRREAVPISDDMDIFSYIQNLPQKPYCILIDECQFLSKKNVYDLARVV
                     DDLDVPVMAFGLKNDFQNNLFEGSKHLLLLADKIDEIKTICQYCSKKATMVLRTENGK
                     PVYEGDQIQIGGNETYIPVCRKHYFNPDI"
     gene            23685..23867
                     /locus_tag="VBC28_000871"
     CDS             23685..23867
                     /locus_tag="VBC28_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019320686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HEO6814872.1"
                     /translation="MPFVKIDLFEGRSQEQKNELAREVTEVVSRIAKAPKENIHVFIN
                     DMPEGTYYPQGELKKK"
     gene            24016..24954
                     /locus_tag="VBC28_000872"
     CDS             24016..24954
                     /locus_tag="VBC28_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001177180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEO6814873.1"
                     /translation="MQLSHSLRLMGTTIDIQINSKNAQKQIQEVIELLELYKNRFSAN
                     DFNSELMAINNNAGIKPIQVHPDLFELITIGKEHSLARPGNLNIAIGPLVQTWRIGFS
                     DAKLPSPSEISEAMILSDPTHILLDSKNQSVFLNQIGMKIDLGALAKGYIADKIIIYL
                     KNEMIDSAIINLGGNVLVHGDNPNRSEGYWVIGIQHPKKKRGQNIGAVKIKNQSVVTS
                     GTYERRLIIDDKEYHHIFDRQTGYPIQTEMASISIVSKQSVDCEIWTTRLFGLSIKEA
                     LDILNAVSYIEGIIITKDDRIYLSDGLKHHFQLFYH"
     gene            24972..25574
                     /locus_tag="VBC28_000873"
     CDS             24972..25574
                     /locus_tag="VBC28_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEO6814874.1"
                     /translation="MKLIGIVGTNSNKSTNRQLLQYMQQHFADKAEIELIEVKDLPLF
                     NKPADKNVPQVILDIAAKIEEADGVIIGTPEYDHSIPSALMSVLAWLSYGIYPLLNKP
                     VMITGASYGTLGSSRAQLQLRQILNAPELKASVLPDEFLLSHSLQAFDKDGNLHDIET
                     SQKLDAIFDDFRLFVKITGKLSNARDLLQKEAENFDWESL"
     gene            25598..26833
                     /locus_tag="VBC28_000874"
     CDS             25598..26833
                     /locus_tag="VBC28_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000673646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEO6814875.1"
                     /translation="MKFVGIVGSNAEQSYNRMLLEFIRKNFKTKFELEVLEIDDIPMF
                     NQVQNWEESFQLRLLNNKITRADGVIIATPEHNHTITAALKSVLEWLSFAVHPLENKP
                     VMIVGASYYDQGTSRAQIHLRKILDAPGVNAYTLPGNEFLLGKAKEAFDDNGNIINPG
                     TVQFLETCLDNFIKYVGVVSSLRKPKAIESEDLYCQGQIDTTITGIDPDDPDWLDKAA
                     EKVNAVEGDAYVKLDNGLLTVNQLNMFLNAMPFELTYADDNNQFLYYNNNHPDPDTML
                     GKRVREQVGNRMATVHGSLPPARMKNVEWVIGTLRNGNQEYVRTVVPGTPPEVINTHN
                     YQAMYYPDGSYAGINEIIFNFKPWLDWYLQTTGQRLVGGTTAAPGASAVDATSSASTS
                     TEDAPVDQATDATSGASEH"
     gene            26932..>27715
                     /locus_tag="VBC28_000875"
     CDS             26932..>27715
                     /locus_tag="VBC28_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HEO6814876.1"
                     /translation="MSLFQEKIAYNCAKKEALYKESLGRYALRSMLAGAYLTMSTAAG
                     IVAADTIGKISPALSGFVFAFIFSFGLIYVLIFNGELATSNMLYLTAGAYNKNISWKK
                     AITILIYCTFFNLVGACILAWLFNQSYSFQHLTNDSFLGHVVAKKLGKPSSGAFLEGI
                     IANMFVNLAILAYMLLKEESAKMTVILSAIFMFVFLSNEHLIANFASFMLAAFSHIEH
                     IKGFTLLNVIRQWTLVFFGNWIGGGVFIGLAYAWLNKTKTSHH"
BASE COUNT         8961 a         5293 c         4395 g         9066 t
ORIGIN      
        1 atttaaaaat tttacaaaga atgtgatggt agcatattca attaaagtta agtaaagaca
       61 taattttaag aaggaaattg tgacttattt atgcaaaaaa gaaagaacca aactggttct
      121 ttcttttttg cttttcagct acttaccaca atttgtaaga aattacaagt agtagtaagc
      181 taagggcacc ttttcaagtg tgacaagaag gttcacacaa cttatcaaga tccgctcctg
      241 cgtttataat ctccctgcgc aatagtggta aaaccacata tcgactcatc gcataacagt
      301 agtttaacat aatttagtta aaatgcaacc tttacttttt aaatataact agattcttca
      361 gtttactttt aatattaaat taaaaataaa ttatttctaa agcaggggag caatagtttt
      421 tgtcactatg ccaattactt tttggaattt tggaatatta tttatatctt tcaagctaac
      481 taaccttgat ttatttaatg tatccatata atctcgataa atgtcttgga ttgcatctac
      541 cttataaaga taaacagcac attcaaaatg gtgatacagg ctacggtaat caagatttat
      601 cgtacctaca acagcctttg tattatcact aaggaatatt tttgaatgaa caaatccaag
      661 ggtgtattca tatattttaa cacctgattt tgttaaagct tgatagtaag ttttagcaag
      721 agcataaggg attggcttat ctgggatacc cggcataata atacgtacat ctacaccacg
      781 ttccgcagca aactgaatgg catgttccaa ttcactgtct aatattaaat aaggtgtcat
      841 gatgtaaaca tattctctag catgattaag aatatctata taaacatttt cccctacttt
      901 atcagtgtct aaaggagaat ctccataagg aataacatag ccttcatttt caactaaatc
      961 atcgtgtaca ccgaggtaag gcgctactaa catttttttc tcagtaatgg accacatctg
     1021 taagaaaagt aggaggaaac tatccactgc tttccccttt aacataatac ctgaatcttt
     1081 ccaatgccca aataactcaa tgtgattaat atactcatcg gctaagttta caccaccagt
     1141 catgcctact actccatcaa taacaacaat ttttcgatgg tcacgatagt tataataagt
     1201 tgaaataaaa ggagaaatcg gagaaaatgc ttttgctttt attcctattt tctcaagtcg
     1261 tttcgtataa tcaaaggata attttgtaat ttctatcata ccatcatata agatacggac
     1321 ttcaacacct tcttccacct tttgttctaa aatactaagt atttctcccc acattattcc
     1381 ttcgtcaatg ataaagaatt ctaaaaagat atacttttta gcttttagta gttcttcttt
     1441 cattttttca aagaatgctt ctcccgttgg tagaaatgtc acatcagtat ttcgataaac
     1501 tgggaaattt ccatgtgctt tttcaaaata ttgaaccaaa tgatatgttg tggatgtact
     1561 tgacttaagc tgattcaaag tttcaggatc atcctctaga taaatctgac tctcatcaat
     1621 caatgtctcc aatctttgtt tcaaaccacg ataaccccag tcaaatttgg tgtacattag
     1681 gaacatcgcc cctagtaaag gggcaatcat taccaatatt aaccaagtta ctcgggacaa
     1741 agcatccatt tcactattaa ctaaatataa gaccgctcca attgttaaga tatgctcaac
     1801 ggttgctaac caaacacgat actgctctag ccatgagtaa gaggctagta aaaacaataa
     1861 ttgtaaaatc aataaaatag cgataacggt tgttcgacta aatatacccc ttagtaaact
     1921 tttcttactc ttattaagta aacgaacaat tttgacttta ttactgataa gtttttcccc
     1981 cctccaaatc aatcatttta ttatagcata ctgtcacttg aatagcattc tattattcta
     2041 aattatctct agttaaaatc taatccccaa taaaaagagg ctagcatcac aagccctacc
     2101 tctttattat ttaaaataat ccaatggctg taccgtcttc taaaacatcc atattgagag
     2161 ctgcaggttt tttaggtaaa ccaggcattg tcaatacgtc tccagtaaga gcaactataa
     2221 aaccagctcc tgtttttgga acaaattcac gaacagttat atcaaagtca gttggagcac
     2281 caagtaaatt tggattatca gaaaagctat attgtgtctt tgccatacaa ataggcatct
     2341 tgtcccagcc attgtcacta aactctttta attgtatttg tgccttaggt ccaaaatgaa
     2401 ctttattacc accatatatt ttagtaacaa tttttttaat tttttcttca atagtgtctt
     2461 catcattata taagcgtttg taattggatg attgtgtttc aatcacatta gcaacagtct
     2521 gtgcaagttc aaggccaccg tctgctccat cttcccacac acttgctaat tcaactgcca
     2581 catcaatatt actacataag gttttaagag ttgcaatttc actttctgta tctgcagtaa
     2641 actgattgat agctacaact accggaacac catattgacg catattgtta acgtgacgct
     2701 cgagatttgt aaaacctctt ttaactgctt tgacgttctc ttgtgaaaga tcttcttttg
     2761 aaacacctcc atgcattttc aatgcacgca atgtagcaac aataacaatc gcatcgggag
     2821 aagtcggaag gtttggcgtt tttatatcaa gaaacttttc agcgcccaag tctgctccaa
     2881 aaccagcttc agtaacaacg taatctgcta agcgtagagc tgttgaagtc gctaagacag
     2941 aattacatcc atgtgcaata ttagcaaatg gtcccccatg gacaagagcc ggtgtcccat
     3001 aaatggtttg aaccaaatta ggtttgatag catcttttag gataagtgtg agagctcctt
     3061 caatcttcaa atctttaaca tagattggtt tacggtccct tgagtaagcc acaacaatat
     3121 ttgacaggcg tttctttaaa tctgataggt cagtagctaa acatagaata gccattattt
     3181 cagaagcaac agtaatatca aagccatctt cacgtggtat cccattgaca gggcttccta
     3241 agccaacgat cacctgacga agagcacgat cgttaagatc aactacacgc ttccaaatca
     3301 ctcgacgttg gtcaatatct aattcattgc cttgatgaat atgattatca agtagagctg
     3361 ataaagcatt attagcagtt gtgattgcgt gcatatcacc agtaaaatgc aaattaatat
     3421 cttccattgg tagtacttgt gcgtatccac ctccagcagc tcctccttta atacccatta
     3481 caggacctaa agaaggttcg cgaagcgcaa tcatcgtttt tttaccaatt ttatttaaag
     3541 catcagctaa tccaatgctc atagtggatt ttccctcacc tgctggtgtc ggattaatag
     3601 cagttactaa aattaacttt cctacttttt gagatcttac agcttcaatc ttatcaaaag
     3661 ataattttgc tttgtattta ccatacaatt ctatatcatc aaaaccaatt cctacttgct
     3721 caacaatctc agcaataggc tttagggcga cgctttgcgc tatttcaata tctgttttca
     3781 tgatattctc cttctaaata ataaaaatag tatagcaata ttccctataa aaacatattt
     3841 ttttgcaaaa aaaatttaaa atgttcgtct tatggaaata aaactgcttt tatttcttct
     3901 gggctagtct gaccaaaata atcttctagt gatacagtac ttaatttttc caagactaat
     3961 tcttctttca tagggcaacc aatgagaagg ttttcaatat ctgaaatatc tttagaactg
     4021 aaaaagtcac cataaatacg gcaattcgta ataatacctt tctcaatttc taagtgaaaa
     4081 tcgtaagttc ctgacggaaa acgagcatct ctgtgatacg aatactgtgg tgagttacca
     4141 taattccaat cccaattctt atatttttca tcagctagag cgtcaatcgc agcccaatct
     4201 ttttcggtta gatgatagcg ttttgcctga tcaatatgat caatatgtaa taattgacaa
     4261 accattaaat ctttaaattg atcggttgtg atatgacgat aatcttctga caagtggcta
     4321 cgaatatctc ctactcgact tcggacagat tttatccctt tagattcaat ttttttccga
     4381 ttaggattta acactttttc catagcatca aaatcaacat ctaataaaag agagtaacca
     4441 ccgtaaatac gaccgtttac tatcgtcatt gctgcacctg atactttttt accatcaata
     4501 actaagtcat ttcgtcccct catttcaaca ctagaagcac ctaaagtttt tagggcctta
     4561 atagctggct cataagctcg tttgaaatca ccaaattgtc catgatcttt cattagatag
     4621 caaatattta cagcacctga gtcaacgtaa acagcccctc caccagtatc tcgacgaaca
     4681 acaggaatat catgttcttt caaatattct tgattagtct ctataactgc attctgaaat
     4741 tttccgattt caactttagg atcacaatag tatggaaata aaatatcatc atctaaaaaa
     4801 acattttgta aaatataaac ttgcatggca agtgcaatcg caccgtctgt gatacgctta
     4861 ccatttctaa ttggttcaat aagatacatt taggtctcct tttatagcaa tttagttatc
     4921 caataatttc ttttttcgct tcagcaatta attcagcgat atgctgtgtc agttgcacac
     4981 tccttgagcg aatcttttga ggaatgcgat aatgcttgtg attaagagta acaaataaag
     5041 agttttcatt taaagagacg aatcgccaaa acggatgctt aataaattgt ggcgtggtat
     5101 gtccaactcc aatctctaga taaagaactt tgtcattttg atgttgtgat aagaaatttt
     5161 cataacgttg tcgttgagca tgaaagtcag catcttccac catacctttt tcttcgttac
     5221 gtttattaat ttcaaacggt tgatcacact ttgggcattt ggggattaag ttactaggaa
     5281 tcttcatatc cttttgttgt gcaatcattt gacgaattgc ctgatcattt cgataagttt
     5341 gttgatgaca atgttgacta cactgccata agccatattc accttgaata tgaaaaactt
     5401 tttctaaatt ataatcagcc acagcgaagg cattatcagc attagttgta ataatatgat
     5461 attctttttt cgctaaaata tcctttaaat caagataagc ttgtcctacc ggttgatcta
     5521 aataattaag tgccacaaaa cgactttgga aggcccaata ttcttcccaa tcttcaaagt
     5581 cataaagact tgcttgcaac atatctaata gttgatattt agcaataaaa tccggaaaag
     5641 cttcttcaaa tctggggcca atataggtga atccatccgc tgcagacatg cctgcaccaa
     5701 taccaactac aatagcatct gattcttcta ttaactgact tagtatttct gattgagaat
     5761 gattagtttt ttctagagtc ttccacactg tcatctattt cctcttttaa taataatttt
     5821 tgataaatct cttggtcttt aggtgtgaat gtattgaaaa taatatagag tttgtgctga
     5881 ttttcgcgtt gccactctaa tatcgcttga acagccaatt cagcagctct ttgattagga
     5941 aaaccaaatt ctcccgttga tatacaaggg aagacaattg ttttaagatt agctcgaact
     6001 gccaactgta agcaggattt ataagaagac tttaataaat cctctcttat acgagatggc
     6061 ttttgatcaa cttttacgta aggtccgact gtatgaataa catacttaca aggaagatga
     6121 taggattcgg taagtttagc ttgtcctacg gcttccattc gtccttgttg tgtcatcaat
     6181 tgatgacatg ctaaacgtaa tcgtgaacct gcaaacgtat gaatatgatt gtcaatacaa
     6241 tggtgattag gaataaaaca acctaacaac ttaccatttg ctgcattaac aatagcatcc
     6301 acttgtaata agcaaatatc cccatggtag gtaaatacat ttgaatatac cgtttgatga
     6361 cagacgtcta tcgtttttac tttttgagta ttccaccaat gtgacaaata cctatcttct
     6421 aaagacaggt actcttgact aatgttttga ggatcttgtt gattaactag agcacgccaa
     6481 accgtttgca aatcggtggt cctcaagtca tcaacgtctg ttaatttttc tgaccgaaga
     6541 tactcaatca tggctagcaa tagttgtttt tgattaggca ttttctaaag cttcaaaagc
     6601 agcataatca acgtctgtta acacaaccaa ccaattttcg tcagcttttt cagaatttaa
     6661 caaagttggt tcttgacttg cagctgtatt aactttaaca actttacctg taagaggaga
     6721 taaaattgcc ataaccgtct ttgaagcttc aatatttaaa ataacatcat caacttctaa
     6781 atttgtatca tctgtgaatt ctacatatcc aacagttccc aagtcatctt gaagttcggg
     6841 agttaagctg atagtataac gttcttcatt tttttcaatt aataagtagt tcgcaatttt
     6901 tttcatgagt attttccttt ctgagaaaac gtgattaata taactgtgat aataatttta
     6961 aggatttgat tcgttgatca agtcctggaa caagaggaat caaaagtagt tcttctgctt
     7021 gactagcatt aactaaagcg tcaaggctgt ctttaacttg ttttggatca cctacaatca
     7081 tacggtggcg atttgattta atttttgctt tttgttcagg agtcaattgg taatgattag
     7141 cttcttcaat agtaggaaat gttgcaaatt cattgaagtc tttatttcct agcatccaga
     7201 tatctaaagt ctttgctatg ttttcagctt cttctgaagt atcagctata actacaaatg
     7261 cagctaccat caaattagga gacttattag gataatattc ttcaaactga tgataataaa
     7321 gagatgaaag ctttttagct tctgtcaatg ggtctttgtc cataaaagga aaaacaccga
     7381 aggtaaagcc taagccaagt ttagccgcta aataagctga tttttgacca ctccccaaca
     7441 catataagtc tgggaagcta gaaagagtcg gttgaactaa tggttcctta ctggatgatt
     7501 catcaataag ccatgacttc aattcctcca gtacctcttc gtaatcatgt gctttatgta
     7561 agctacgaag tgcatttgaa actttaactg tccctagtga atttcctaaa ccaatactta
     7621 ctcgattcgg atgacatgtc tctaatgtct tgagagtctc cgcgagttta aaactactat
     7681 agtgcagagg cattatacct ccagagccaa ttttgataga tttagtctgg ttagccaaat
     7741 gcattatcat taattctgga ttgctaatac tgaatgcctt aacaccgtga tgttcagcca
     7801 cccaaaattg atgatatcct agtcgctccg cctcttgtga taaaagaatc gtttcttgta
     7861 ttgcatcact tgcagttttt ccataatcaa taagcccata atcaaggaca cttaatttca
     7921 tctttcctcc ctataattgc ttcgaagttc tctaaaaact tctcgagctt cctttggtaa
     7981 agattctcca taatccatca aaggaacaat atctttaaag gaggcttttg gaatcgccaa
     8041 accttctaaa tcactcactt taatccttaa attaatctga tcagaacgct tagcttttat
     8101 tttttgtaaa aattccggat caactactaa gggcgtggaa gctcctacca tatgagcatg
     8161 ttgtaaggct tcaaacacct ttgaagcact attaatacct ccagttgcca taattggaac
     8221 tctatcacca aaatgttcaa atataacttg attaaccagc tgccctttgt aaactccctc
     8281 agaacgtata gtatttctaa agacatcatg cccccagctt gcaatagcaa gataatctac
     8341 ctgagcaatc gctaaaattt tttcaagaaa ttccataaac tcttcaatgc tatatccaat
     8401 ttgtgaaccg cgagtttctt caggcgtcgc tctaaaacca aggataaaat ctgactctgc
     8461 ttcttctctg ataacttttt gtaccgcttt aaaaacttct aagcctaaac gacagcgatt
     8521 agtcaaagtt tgaggaccat actcatcttt tctttgattt gagaaggttg agaaaaaagt
     8581 ttgaattagt aatcgttgtg cagaagatat ttcaacacca tcaaatccag cttgtatagc
     8641 tcgtcttgta gcctggacat attcatcgat aatcattagt atatctttgt gtgttagttc
     8701 tttaacctga tgtgggtatg gcgattgcaa ttgcataggg ctgggaccat atacatatcc
     8761 gtgtcttgcc aaagtgtgac ttgagaaacg tccagcatgc gtcagctgca aaacagcttt
     8821 cgctccctta gacttcattg ctttagccaa tttagtcaaa ccaggaatgt cctcatcctt
     8881 tgaaacgcta aaaccatatt caaataactg accatattct gtaacatatg ctgctccagt
     8941 aatttgtaat ggagcggatt tagcacgacg aacagcatat gctatgtcgt catcagttac
     9001 aaaaccttct gatgtagaag aattagttac cataggtgat agaacaaaac gattttctaa
     9061 gctcaatcca ttgggaagta ttaatggacg aaataaatta ccttggacat tattcatctc
     9121 tgctcaccgt ccccttatta gccatagact tagcctgaaa tctatcaaac tctgcaacta
     9181 aatcgggcct aatcatcagt aaacgatcat taaaacctac ttgtttaagg ctaatcaaca
     9241 tacgatcaat aacatcttct actgtcatag gacaatctaa tagatcagat agattagcca
     9301 taatttcagg atctacattt ggataagcaa ttggactacc cgtatcacct agaccaatct
     9361 tataaaaatc tgaaatcatt cgactccgct ctttttgatc gccacaaacg ctaaggtaaa
     9421 tcgataccgc aataccattc tttatacggc gctgagccaa gccagcaaat tttttgccat
     9481 ttatactaag atcaaactta ccaggacaat aggaggtctc tacttcaaag tgctcaatag
     9541 gttgataaaa atctgaaaat atacttctaa taaaatcgac cattataaga tacccatctg
     9601 agatagacaa ttttctctca aaaacatctg gaataatcaa tgaaaaattt aaaattcctt
     9661 catcagctac aactgcgaga ccaccaaaat tccgaacaac tggttcatag ccacgactga
     9721 tgatttcttt tttagctaat tctaaatgtg gtaattctcg atctaacatc cccagaatga
     9781 ctgtcttagt catcggccaa atatgcaaaa taatttcttg atttgagcga ttaatctccc
     9841 ttaaaaatac ttctgtccat ataaaaggtt tttccgcgtc ttgagcatct gtaacatagt
     9901 ctttaaaaat agatacaggt aactgcgcta gatcttgcca ttccaataaa tctgacctcc
     9961 tttctctaga aaactctgca agatttattt tatcgcaatc gcttgaaatt ttccagtaag
    10021 tttccctcaa attccattac tatcaagaag aaatatatct tttatttacg aaaagcattt
    10081 ttttatgtac aatatggcta tgaaaatttt gattacatca ggtggaacta ccgagaaaat
    10141 tgataccgtt aggagtatca cgaatcacgc aacaggagct ttaggaaaaa taattgcaga
    10201 aaaatattta cgagaaggac atcaggtgac actcgtgact actaaaaatg caatcaaacc
    10261 agaatctgct accaacttat ccatatttga gattgaggac gttgactcat taataaaaac
    10321 cttgaagcca ttagtgaaag agcatgatat tttaattcat agcatggcag tgtctgacta
    10381 tacccccgtc tacatggctg atttcgaaaa ggttaaatca agcgatgatt tagatacttt
    10441 tttgcgcaaa gataaccacg aagggaaaat atcatctgaa tcagaatatc aagtcctttt
    10501 tctcaaaaaa acaccaaaag ttatcagcct tgtaaaaaaa tggaatcctc aaattattct
    10561 agtaggtttt aaactacttg tgaacgttac taaagaaaat ctttttaaag ttgctcgtca
    10621 tagcttaatc aaaaataaag ctacgtttat tttagctaat gacctcattg atattacctc
    10681 aaaacatcac atagcctacc tacttgatca tgataatgtg tataaagcta ccaccaaaga
    10741 agacattgct caattaattt acgaaaaggt aaaaaaatat gattaaacgt attacactag
    10801 ccgtcaccgg ttcaatttcg gcctataaag cagcagacct tacaagtcaa ttaactaaaa
    10861 ttggctatga tgttcatatc ataatgacac aagctgccac agaatttatt acaccgttaa
    10921 ctctgcaagt attatcaaaa aatcctattc atttagatgt tatggatgag cataacccaa
    10981 aaatcatcaa ccatatcgaa ttagcaaaac gcacagattt atttatcgtt gcacctgcct
    11041 ctgctaatac tattgctcat ctagcatatg gcttcgctga taacatcgta acaagcgttg
    11101 ctcttgctat gcctgatgaa actccaaaac ttatcgcacc agcaatgaat acaaagatgt
    11161 atcataatac aatcacccaa cgtaacattg atattttgaa aaaaataggt tatcaggaaa
    11221 ttgaacctcg tattagtttg cttgcctgtg gtgataccgg tcaaggagcc ctagctgata
    11281 tctctactat acttaaatgt atacaagaag ttgcaaatta ggctgtaaat ttgtatacta
    11341 tttctagttt aatttaagga gttataatga aaaagaaaaa caaatcctct aacattgcta
    11401 taattgcaat cttttttgct attatgcttg tcattcattt tttgtcatca tttattttta
    11461 gtttttggtt agtccccatt aaacctactt tgatgcatat cccagttatt attgcatcta
    11521 tagcctatgg acctcgtatt ggtgcaactc taggcgcctt aatgggtggg atcagcgtag
    11581 ctaacagcag cattgttcta ttaccaacga gttacctctt ctcacctttt gttgaaaatg
    11641 gtaattttta ttcgctaatt attgcacttg taccacgtat tctaatcggg attattcctt
    11701 atttcgttta caaattacta cacaaccgct ttggtttggc tatctcaggt gctataggct
    11761 ctctaacaaa cacagtattt gttttatctg gaatttttat ctttttttca agtacttata
    11821 atgggaatat caagctaatg ctcgctggga ttatttcatc taattcatta gctgagatgg
    11881 tcattgcagt tatcattgta tatctaactg ttcctcgtat tctcaatatt aaacattaaa
    11941 aactggccag gaagaaatct tcctggcttt tcttacttat attttatagt catattgaaa
    12001 aaaacatgtt ataatgaaag cgtttataaa tattgaagga gtatttcaat gacttacaca
    12061 gaaaattttc aaaaatggtt agattttgag cagcttcctg actatcttcg tcaagaatta
    12121 ctatcaatgg atgaaaaaac aaaagaagat gctttttaca caaacttaga atttggtact
    12181 gctggtatgc gtggttatat tggtgctggt acaaatcgta ttaatattta tgttgtccgt
    12241 caagcaaccg aaggattggc aaaattaatc gaaactaagg gagaggaagc taaaaaacgt
    12301 ggtgtagcta tcgcttatga ttcgcgccac ttctctccag aatttgcttt tgagtctgca
    12361 caggtcttgg cacagcatgg aattaaatct tacgtatttg aagctcttcg tccaacacca
    12421 gaactctctt ttgctgttcg ccatttaaat gcctatgctg gtattatggt tactgcaagc
    12481 cacaatccag cacctttcaa tggttataaa gtctacggac aagacggagg tcaactacca
    12541 ccagctgatg ctgatgcact aacagacttt atccgtgcta ttgaaaatcc atttgcagtt
    12601 gaacttgctg atttagatga aagcaaatca gcgggtctta ttcaagttat cggtgaagat
    12661 gttgatattg aatatcttag agaggttaaa gatgttaata tcaatcaaga tttaatcaat
    12721 aactttggaa aagacatgaa aattgtctat actcctctcc acggaactgg tgagatgttg
    12781 acacgccgtg cccttgctca agccggtttt gaatctgttg ttgtagtaga atcacaagct
    12841 aaggcagatc cagacttttc tactgtcaaa tctcctaacc ctgaaagcca agctgccttt
    12901 gctcttgctg aagaactcgg acgagaagtt gatgcagatg tcttagtagc tactgatcct
    12961 gatgcagacc gcctaggcgt tgaaattcgc caaccagatg gttcttataa aaacctttct
    13021 ggaaatcaaa ttggagcgat tattgcgaaa tacatcctgg aagctcataa aacagcagga
    13081 acacttccag aaaatgctgc tctcgctaaa tcaatcgtat caacagaatt agtaacaaaa
    13141 attgccgaaa gttatggcgc tacaatgttt aacgttttaa ctggatttaa attcatcgct
    13201 gagaaaattc aagagtttga agaaaaacat aaccacacct atatgtttgg ttttgaagaa
    13261 agcttcggat atcttattaa accatttgtc cgtgataaag acgctattca agcagttctt
    13321 ttggttgctg agattgcagc ttattaccgt tcgcgtggtt taacacttgc agatggtatt
    13381 gatgaaattt acaaagaata tggttatttt gctgaaaaaa cgatttcagt aacactttca
    13441 ggagtggatg gagctgctga aattaagaag attatggaca aattccgtga aaatggtcca
    13501 aaacaattta acaatactga tattgtatta ttagaagatt tccaaaaaca aaccgctact
    13561 aaaaatgatg gaataatatc taacttaaca actcctccaa gtaatgtttt gaagtacacg
    13621 ttagctgatg attcatggat tgcagttcgc ccttcaggaa ctgaaccaaa aatcaagttc
    13681 tatattgcta cggtcggtaa tgatttagca gatgcagaaa caaaaatcgc caatattgaa
    13741 aaagagatta caacatttgt aaattaataa aaaaagctag gaacttactc ctagcttttt
    13801 ttatatctat ctaacgatgt tctattattt tcaattatat gaaatataac tgcaattaac
    13861 ttcacgatca aataatagag cagacttaca attaaccacg ttatcccaat aactgcaact
    13921 aaacctatta aaacagataa taccgtattc ttagttgcta gttggttact gataaaccat
    13981 gaaactgcag aaataaatat cagtaataat agttgaagcc tttttttcat ataattcctc
    14041 ttactacttt tgtgcatttt gcatttgata ataaatacct ttttgagcta ttaattcaga
    14101 gtggttaccc acttctacaa tttcaccaga taccataact aaaataatat ctgctgtttg
    14161 aatggtagat aagcgatgtg caataataaa gcttgttctc cccttcatca acatttggaa
    14221 tgcttcttga acaagaactt cagtacgagt atctatagac gatgttgcct catctaatat
    14281 caatattcta ggtaatttaa gaaaaatacg agcaattgtc aatagctgac attgcccctg
    14341 tgataacgag tctccagcat cttctaaata cgtatcatat ccattcggta attgtttgat
    14401 aaagaaatct gcattagctg cttttgcagc ctctatcact tcgtcacggc tagctttagg
    14461 attggcatat gcaatgttat cgtgaatcgt agcacttttt aaccacgttt cttgtaatac
    14521 cattccaatt tcttgcctta attgtgatgg ctcataatca gtaataggct taccatctaa
    14581 aaggatattt cccccgtcaa cttcatagaa acgcattaat aaattgatta aagtagactt
    14641 cccagcacct gttggtccta caatagcgac cttagctcct gctggaatgt gtaaattaat
    14701 accattcaat agtggttttg atttattata accaaaaaca acatttttaa aatcgatttg
    14761 acccttgacc gttgatgaat caagtttttc tgtccctgtt atatttggtg acgattcttc
    14821 taaaatagag taaaggcgtt ctgcacaagc cagagcactt tgcatctcag acaggacgga
    14881 agagatgtca ttaaatggct tagtatattg attgacataa tttaagaaag tgattaattg
    14941 accaacacta aaagcccctg acataatacg aagggcacca atcccagcta agaagccata
    15001 aatcaaacta ttgataaatc gggttgatgg attgacagtt gaagaataaa aaattgctga
    15061 ttgagaaaag ttagcatact cttgattaat agtcctaaaa tgattactac tttcttcttg
    15121 cgcactgaat gcttgaataa ggctttcttg actcaccatc tcttcaatga attgagtttg
    15181 tcgtcctcgt gatgccgttt gattttgata taagtgataa ctctttttag caataaagcg
    15241 ggctagaaag agtgataaag gcgtcagaaa taataccaaa aaaagcatta ataaatcaat
    15301 ctttgccata ctaaaaatag taactagaat agttaataaa ccaacaaaga actggttaaa
    15361 aaccattaat aaaccatttg ataattgctc cgtatcggta gtcacacgac taatcaagtc
    15421 acctatgcca cgtttatcaa ggtaagctat aggcaataga ttcaactttt ccattacagc
    15481 ctttcttaaa gaagcaacat aatgaaaaat taagcggtta tagagcagag gattaatcca
    15541 ttgaataata gcatttgcta aaatgactgc aatcatcttc cacattatag gtaacaataa
    15601 tatcatagaa tgaggcgata atacaacgtc taccgcttgt ccaattagaa caggcaaata
    15661 aacagtgagg caaacttgta caaccgtccc taataatgcc attcctacta agcttttttt
    15721 agaaagaaga tcttgaacaa gctttttttg tatcttttta ggatgacttg ctttcattta
    15781 ctcctcctcc ttaaaatttt gtgaatggta aatggactta taaactttgt tatgagctaa
    15841 taaagattga tgattatcaa aaccaacttg tttcccctta tcaaggagta agattctatc
    15901 tgcattttgt atactattaa ttctctgtga tacaataata agattagttt ggttgaaatg
    15961 tttagttatg gctttaaata aacgagcttc tgttaaataa tccaatgcag atgttgcatc
    16021 gtctaaaatc aaaaatggta tcttatcttg aactaaagct cttgcaatcg tcaacctttg
    16081 cctttgacca ccagagaaat ttctaccaaa actctctaca ggggcatcaa gttgaccatc
    16141 tttttctttt acaaaatcac tagcttgtgc tatttctaaa gcagtccaaa gtttttcctc
    16201 actaacttta ccaagtccca aagaaaggtt agaacgaata gttcctttaa acaactgagc
    16261 attttgagga acaactctta ccagggtacg ccaatttgaa attgtatctg gactttttcc
    16321 ctgatgatat atatcaatat ccccttcttg cactttataa atatgaagca gtaaattaac
    16381 caaggttgat ttcccagagc cagttccacc aattatccct aaagtttctc ctacattcat
    16441 agaaaaagta atatcagata aagctttttc agaagaattt ggataagaaa atgccatttc
    16501 ttgtacttct aaagccttat tcgaatattt tggctcaagt ttgtcatcaa ttatttcaga
    16561 cggtctttca aaaacggcta taattcgctt agcactgata taactttggt tcagtgatgt
    16621 caccaacatt gtcattttta acaattcaac aagaatttgc aataagtaat taattaaagc
    16681 aaccaacatt ccttgtgata aaagatgatt agctatattt aaattaccac gccaaataat
    16741 cacaaccaaa gtgatattaa caactagaaa tgttagaggc gttactaaac tagaaagcct
    16801 acctgctttt aattgtaaat tcgtataatg ataattgata tcattaaatg cttcactctc
    16861 tctatctact tgattaaagg cacggataac tcgtacacct tgaagttgtt gcctagtcaa
    16921 ctttaccaaa taatcggttg aagttctgat tttaaggtaa ataggattta atagtcgaga
    16981 cattacaaaa acaatgataa ataacgtgac aaccattacc aagaaccaaa tagtcaatga
    17041 ggggctaatg gaaaaagcca taattatggc accaaaaaca ataatcggag ccctcaaaaa
    17101 taaacgtaaa aattgattta aaccagtttg gatttgaaat gtatcagcag tcaaacgagt
    17161 aatcaaacta gcagttccca attcatctct gtcttttttc cccagtgaca tgactttttg
    17221 gtagagatcc tctgtcaatt gtctcgtata accaactgcg gcttttgaag aataatattg
    17281 agcagttatc gctactacaa cacccaccgc agccaaaaag aaaatcaatc caatttgcaa
    17341 cagtaaacta cttctatccc ctcgtggaat ggtctcgtca atcatttttg caataacgat
    17401 tggtaccaaa agttcaaaag aggcttctaa taatttgaaa aggggtccaa agatagtttc
    17461 tttgatatac cccttaaaat aataagttag tcttttcata cttacccttc ttaaaacaac
    17521 tccattattt ttatcaaata aaggttgaat tgaacttcac gtgagtagta aatattacca
    17581 ccatttttat agagcttccc tcctgaaatt aaggcgagag gatgatagta aaagtaagtg
    17641 tccccgttat aatttcctag gctgggagct acaacatttt tagagtaatt tttggccaac
    17701 tcaatggaat tttcaccccc atgatccgct acataatcaa tataagaaga accaaaattg
    17761 tatgcttgga cagcagtcca tacatcaacc ttagcttttt tagcttgact tatattttgt
    17821 gacaacagag tgacgccatg tttaatactg gtgtggctat ccgtaatact gttcgtagtt
    17881 ccactgatac tttcacttga ttgcatcaca tctatagcat ctcccttagt ttcagtataa
    17941 atcatagata atactaagtg cacattagcc tctgaaccat tctctgcaag agtattctgc
    18001 actaaatcat gatattgaag cactttttta acattttcat ggatgataac cagacggtac
    18061 ccaatgaaaa taataataat taatgctatc aaacgtttta aaaatttaaa catattattt
    18121 attttgaata tcctgtatgt ttttgattag tattgagtat gtaccatcag ggttttggat
    18181 aaattcaact gattcggcat cttggtagac attgttgggt acaatcaatt caataccatt
    18241 tgaaagagat agcttctgat tctctaactt cttaatttga cgggaatgat caatatcact
    18301 tacttgaaca ggtccaggta ttgcatcttt caattcatca acaaaagtca gcctggctgt
    18361 taaattatca tcaaataatt gatctgctaa tttttctgga gacaattctt gctcttcttc
    18421 taaattctta taaatagccg atttcatttt tgattggaaa gaaaaatcat ctttgttgaa
    18481 attttcagca attttttgag cagtctgttc aaccattttg atagattttt taaccgactg
    18541 ttctggttgt acttgtaata aattttcaga aaaataatta gcaaatgaac cattgtgttt
    18601 gatacgcttt tcaattaaat agtattgctt tgatgacttg ttaacaacca aagcttcatc
    18661 tggagtttga gcagcacttg gaaggttatt ctgtgttatt gtaattggct gctcttgatt
    18721 ttcagaaaca tgagcaaatt gttcctttaa tgagattcga agaaaagcaa aatgttccat
    18781 tccatccttg tcaaattgaa taaaaactaa gtcattcgtt ttttgatctt ccgatatgac
    18841 aaactcttct ttccaaagtt gcgcaatttg acaggaagaa acatataaat catcttgtaa
    18901 atgactcata aatacattat cctctccaaa atagccacgc ttagcttcat cagaaaaaac
    18961 tttacttaat tttttacgaa agtagtcatc aatccttgga gttagggtta acggagtatc
    19021 tgatagcact aactctgtgt cgttaggaga aaactgatgt ataataatct gtttgatata
    19081 aaaatccatc ataacattaa ttctcataca aggggaaagc gtccgttaat gatttaattt
    19141 cttgacgaac ttctgtgagg atatcttgat tttcatgatt tttgagtgcc ttaatcatta
    19201 attcagcgat tcttcgagat tcttcaactc ccattcctcg actagtaatt gctggagtac
    19261 caattcgaat acctgatgtt ttaaatggtg atagtcgctc aaacggtata gagttcttgt
    19321 taagagtgat attgacttct tcaagaacat tttgtgcctt tttgccattc tcaataactt
    19381 ttgtgacgtc tactaagaac aaatgattat cagtaccatc agaaatcaaa tgaaagtcat
    19441 catcttcctt aaaaacttta gccatggctt gagcattttt aataatatcc tcaccgtata
    19501 ttttaaagga aggatctagg gcttctttca aagctacagc ttttgcagca attacatgtt
    19561 ctaatggtcc accttggagt cctggaaaga ctgcagaatt aattttttta gcaattgctt
    19621 catcattggt taaaatcaaa ccacctctag gtccacgtag agtcttatgt gttgtcgttg
    19681 tcgttacatg agcataagga ataggactag ggtgatgacc agaagcaact aaacctgcaa
    19741 tgtgagccat atcaaccatt aagtaagcat ctactgcgtc tgctatttga cgaaattttt
    19801 caaaatcaat aatgcgagaa taagccgatg cccctgcaac aattaattta ggctgcgttt
    19861 cctgtgctat tttcaaaatg ttatcatagt ctagcatctc cgtcttagga tcaacagaat
    19921 aagaaacaaa atgataagtt tttcctgaga agctaactga agcgccatgt gttaagtgcc
    19981 cacctgctgc taagtccatc cctaagacag tatctcccgg ctcgattaaa gccatgtaag
    20041 ctgctgcatt agcctgacta ccagaatgtg gttgaacatt agcaaactca gcattaaata
    20101 acgtcttagc tcgttctata gctaaacttt caactacatc aacacaatct gttcctccat
    20161 aataacgatg actaggatac ccttcggcat atttatttgt caaaacagaa ccttgtgctg
    20221 ccataacagc ttttgaaaca acgttttctg atgcaattaa ttcaatatta ttttgttggc
    20281 gaatttcctc atcatgaata gcctgccaaa gttcttggtc aaattcttta aaattgtctt
    20341 tatcaaaaat cattttttac tccttaagct cgttataact tttaaataat ggtttcaatc
    20401 attatattta tcacattttt actattagtt gcaagatgat taaattaatt ttcaaaaatt
    20461 aattcaggta tcgtagccgt tagtacctct tgcgtgattg caccttgcct taaaatctta
    20521 actctatcac cagacaaatc aataatagta gaatccacac ctgtcaatgc tttatcatct
    20581 tcaataccta aaacttgatg attaaactgt ttttgaattt ctgagaacac acgaccacta
    20641 tcacatccag aaatattagc agaaggacca atcagtggac catggttagc aatcatatca
    20701 agggttacag ggtgcttagg catccgaaat cctacgctta ataacccaga attgatccaa
    20761 tgcggaactt cttgtgaagc ttccaaaata atggttagcg gtcctggtaa aaaagcatcg
    20821 tacaattgct ttaaataggt cggttgattt ttagaatatt ttaaaatagt ctcaaaatca
    20881 catatattta agttcatagc cttatcacga ggcctttttt taagctcata gacgtggttc
    20941 acagcttctt cactcaaagc tttggcaaaa attccgtaga ctgtttctgt tggcataata
    21001 actgcgccat gatcctctaa aatctgtccc aaatcattca ttatctaata ccaccattct
    21061 atctttacca aatatatctt tcaaaactct gcaacgattg tttggaaaat atcttttcaa
    21121 taaacttcgt aaatcatcac cttgtttgta acctatttca aaatataatt tacctctagg
    21181 ttgcaaatat tctcttgagt tttcaatgat tttacgataa atagctaatc cttcttcgtc
    21241 tgcaaaaagg gcactatgag gctctgaagc tagcacattc ttccccacct cgtctttgtc
    21301 attgtaagaa atatagggag gattagacac aataatatca aatttacctg aaatgttact
    21361 aaagacatct gattctataa aagtcacttc cgcatcacaa ttattagcat tttcctttgc
    21421 caagtctaat gcagatacag aaatgtcact agctagaaca tcccaagagg ggcgttcttt
    21481 ttttagagaa atggcaattg ctccactacc tgtaccaata tctaaaacag aacaatcttc
    21541 tactttattc tctgataaga ttagatcaac caactcctct gtttcaggcc tcggaattaa
    21601 aactcgttca tcaacaaaga aaataaggtc tctaaagtaa gctttacctg taatatactg
    21661 cggtgatcta tgttttttaa gctgttgaaa aatattttcc attaattgct gatcagtatc
    21721 actcacacta ctattttgat ttaaaaggta atcagttgtt gaccattgtt ttaggtcttt
    21781 taatacataa ataaaatttt cgacttcttc accacaagct tctaataact gtccgtaatg
    21841 cttgattaac tgcgcataat tcattattta tttaaggctt ctaacttttg agtctgatca
    21901 tacataacaa gtgcatcaat aacttcatcc atttttccag ataaaatagt atctaatttt
    21961 tgaagtgtca aaccaatacg atgatctgtt acacgatttt gtgggaaatt ataagtgcga
    22021 atacgttctg aacgatcacc agtaccaact gttgatttac gttcagcatc ttgttcatct
    22081 tgagcaattt gcgcaaaatg gtcagcaaca cgagcacgaa tgattttcat cgctttatca
    22141 cggttttttt gctgagtacg ttcttcttgc atttcaactt tgattcctgt aggaatgtgt
    22201 accatacgaa ctgcagtagc aactttattg acgttctgac caccagcacc agacgcgtgg
    22261 taaatatcga cgcgaaggtc tttttgatca atttcgtatt ctacttcttc aacttctggc
    22321 attaccaaaa cagttgcagt tgaagtatga acacgaccct gactctctgt caccgggaca
    22381 cgttgaacac gatgagctcc tgattcatat ttaagcttag aataaactga ctgaccagaa
    22441 accatagcaa ccacttcttt aataccacca acaccattaa cggatgattc cataacttca
    22501 aaacgccatc cttgagtttc agcatatttt tgatacattg ttaaaagatc accagcaaaa
    22561 agcgctgctt catcgccacc agcagcacca cgaatttcta aaataatgtt tttatcatcg
    22621 ttaggatctt taggtaatag aaggattttt aatctttctt catactcttc tttagctgct
    22681 tttgattctt taagttcttc tttagccatt tcttccaatt cagcatcacc agaagcatcc
    22741 ttgatcattt cttcagcatc tgaaatattt tgaatgactt gcttatattc tctataagct
    22801 gtgacagttt ctcgagtatt agcttcttca cgtgataatt ccataaatcg ctttgtatct
    22861 gaaacaacat ctggatcact aagtaactca cctaactctt cataacgatc ttcaactgcc
    22921 tgcaattgat cataaatatt catttttctt tgtatctcct ttaaaaccga ttatctttta
    22981 aatatctgga ttaaaataat gttttcgaca aactgggata tatgtttcat taccaccaat
    23041 ttgtatctga tcaccttcat agacaggttt tccgttttct gtccttaaaa ccatggttgc
    23101 tttcttacta cagtattggc aaattgtttt gatttcatca atcttatcgg ccaataatag
    23161 caaatgtttt gaaccttcaa aaagattgtt ttggaaatca tttttcaaac caaaagccat
    23221 gactggtaca tctaagtcat ctactactct agctaaatcg tagacgtttt tcttactcaa
    23281 aaattgacat tcatcaatta aaatacaata aggcttttgt ggtaaattct gaatatagga
    23341 gaaaatgtcc atatcatctg aaattggaac tgcttcacga cgcattccaa tacgacttga
    23401 aacgactcca aattcatctc gagtatccaa ggagcttgtc ataataacca caggttttcc
    23461 ttgttcttca tagttatgag caacttttaa aatttcaatg gttttaccag aattcattgt
    23521 cccatattta taatagagtt gagccaaagc ggaaaatcct actttctata gtattctacc
    23581 tattttacca caaaggctga agttttccca gccattgaaa acagtgtgta ctttttcaca
    23641 acattatgtt acaatgatgc ccaataacat aaaggagtaa cattatgcca tttgtaaaaa
    23701 ttgacttgtt tgagggacgc tcacaagaac aaaaaaatga attagctcgc gaggttactg
    23761 aagtagtatc acgcattgca aaagcaccta aagaaaatat ccatgttttt atcaacgata
    23821 tgcctgaagg aacctactat cctcaaggtg aacttaagaa aaaataagaa atagtaaaag
    23881 aggttaccca ggtaacctct tttgatagtc tttgcaagca tatttcttga caaattgaaa
    23941 atatttgaaa ataagtattg attttcaagt tagaaaacgg tatcattaga atatattgct
    24001 ttgggggtgt aaaatttgca actttctcat agtttaagac tcatgggaac gactatcgat
    24061 attcaaatta attctaaaaa tgctcaaaaa caaatccaag aggtcataga actcttggag
    24121 ctctacaaaa atcgctttag tgcaaatgac tttaattcag agttaatggc tataaataat
    24181 aacgctggta tcaagcctat tcaagtacat ccagacctct ttgaattaat cacaattggg
    24241 aaagagcaca gcttagctag gcctggtaac ctaaatattg ctattggtcc tttagtgcaa
    24301 acttggagaa ttggattttc agatgctaaa ctacctagcc ctagtgaaat atcagaagct
    24361 atgattttat ctgacccaac tcatatactt ctagattcaa aaaatcaatc tgttttttta
    24421 aatcagatag gaatgaaaat tgatttgggt gcacttgcaa aaggatatat cgctgacaaa
    24481 attataatct atctcaaaaa tgagatgatt gattctgcta tcattaactt aggcggaaac
    24541 gttttagttc acggagataa tcctaatcgt tcagaagggt attgggttat cggtattcaa
    24601 catccgaaaa agaaacgggg acaaaatatt ggagctgtca aaattaaaaa ccaatctgtt
    24661 gtaacctcag gtacttatga acgccgtcta attattgacg ataaggaata ccatcatatt
    24721 tttgatcgac agactggtta tccgatacaa acagagatgg ctagcatttc tattgtttct
    24781 aaacaatccg tcgactgtga aatctggacc acaaggttgt ttggcttatc cattaaagag
    24841 gctcttgata ttttaaacgc tgtttcctac attgaaggca ttatcatcac taaagatgat
    24901 cgtatctatt tatctgatgg cttaaaacat cattttcaac tattttatca ttaagaaaga
    24961 ggatttctaa tatgaaactt attggcatcg ttggaactaa ctcaaataaa tcaacaaatc
    25021 gtcaattatt acaatacatg caacaacact ttgctgacaa agcagaaatt gaactcatcg
    25081 aagttaaaga ccttccgtta tttaataaac ctgctgataa aaatgttccc caagtaattt
    25141 tagatattgc tgctaaaatt gaagaagctg atggtgttat tattggtacg ccagaatatg
    25201 atcattctat accttcggcc ttaatgagtg ttttagcttg gctatcatat ggtatttacc
    25261 cacttctcaa caaacccgtt atgattactg gtgcttcata tggaacactt ggatcatcac
    25321 gtgcacaatt acaattacgt caaatactta acgctcctga actaaaagca agtgttttac
    25381 ctgacgaatt cttgttatca cactctctcc aagcctttga taaagatggc aacctacacg
    25441 atatcgaaac aagtcagaag ctagatgcta tctttgatga ttttcgtctt tttgttaaga
    25501 ttactggtaa attatcaaat gctcgtgatt tacttcaaaa agaagcagaa aattttgact
    25561 gggaaagttt ataagagtat agaagggaat aactaagatg aaatttgtag gtattgttgg
    25621 ctcaaatgca gagcaatcat ataatcgtat gttgcttgaa tttattcgca agaattttaa
    25681 aaccaaattt gaattggaag tccttgaaat cgatgatatt ccaatgttta atcaagtcca
    25741 aaattgggaa gaaagtttcc aacttcgtct tcttaataat aagatcacac gagctgatgg
    25801 cgttattatt gccactcctg aacataatca tactattacg gccgctctta aaagtgtgct
    25861 tgagtggctc tcatttgctg tgcatccact cgaaaataaa cctgttatga ttgtcggtgc
    25921 ttcatattat gatcaaggca catcacgtgc tcaaatacac cttcgtaaaa tccttgatgc
    25981 tcctggcgtt aatgcttaca cacttccagg aaatgaattc ttattaggaa aagctaaaga
    26041 agcctttgat gataatggaa acattatcaa tcctgggact gttcaatttt tagaaacttg
    26101 tcttgataat tttatcaaat atgtaggagt tgtatcatca ttgagaaaac ctaaagctat
    26161 tgaatctgaa gacctctact gtcagggaca aatcgataca accattactg gtatcgatcc
    26221 agatgatcct gattggttag acaaggcagc cgagaaagtt aacgccgttg aaggggatgc
    26281 ttacgttaaa ctagacaatg gtttacttac tgttaaccaa cttaatatgt ttttaaatgc
    26341 tatgccattt gagctaacat atgcagatga taataatcaa tttctatatt acaataataa
    26401 tcatccagat ccagatacaa tgttaggaaa acgtgttcgt gaacaagtag gaaaccgtat
    26461 ggccacagtt catggtagtt taccaccggc acgaatgaaa aatgttgaat gggttatcgg
    26521 tactcttcgt aatggaaatc aagaatatgt acgtacagtc gttcctggta cacctccaga
    26581 agttatcaat actcacaact atcaagctat gtactaccca gacggatctt atgctggtat
    26641 taatgaaatt atctttaact ttaaaccgtg gttagattgg tacctccaaa ctactggaca
    26701 acgtttggtc ggtggaacta ctgctgctcc tggagcttca gcagttgacg ctacttctag
    26761 cgcttcaact agcacagagg atgctcccgt tgatcaagcc acagatgcca cttctggtgc
    26821 ctctgaacat taatctcctc gcttttgagg atctgatacc ttaaaaagat atcaagatcc
    26881 tcctttttaa gatttaatca agttaataaa atcacataaa aggaatatac tatgtcactt
    26941 tttcaagaaa aaattgctta caattgcgct aaaaaggaag cgctttataa agagagttta
    27001 ggacgctacg ccttgagatc aatgctagca ggggcttatt tgacaatgag tactgctgcc
    27061 ggtatcgtcg cagctgatac tattggtaaa atttctcctg ctctatcagg ttttgtattt
    27121 gctttcatct ttagttttgg acttatttat gttttaatat ttaatggtga attggcgaca
    27181 tctaatatgc tttatctcac tgcaggagcc tataataaaa atatctcttg gaaaaaagcc
    27241 ataacaattt taatttattg tacttttttc aacctcgttg gtgcttgtat attagcttgg
    27301 ttgtttaatc aatcgtactc attccagcat ttaacaaatg atagcttttt agggcatgtc
    27361 gttgctaaaa agttaggaaa acctagttca ggagctttcc tagaaggtat tattgctaat
    27421 atgtttgtga atttagctat ccttgcttac atgttattaa aagaagaatc ggcaaaaatg
    27481 acggttattt tatctgctat ttttatgttt gtttttctat caaacgaaca cttaattgct
    27541 aattttgcat cctttatgtt agctgctttt agccatatcg aacatattaa aggttttact
    27601 cttctaaacg ttatccgcca atggacactt gtattttttg gtaactggat aggagggggc
    27661 gttttcatcg gcctagccta tgcttggtta aataaaacaa aaacttctca ccatg
//
LOCUS       DATRMA010000022        27447 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.022, whole genome shotgun sequence.
ACCESSION   DATRMA010000022 DATRMA010000000
VERSION     DATRMA010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 27447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27447
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.022"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            21..218
                     /locus_tag="VBC28_000876"
     CDS             21..218
                     /locus_tag="VBC28_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease"
                     /protein_id="HEO6814877.1"
                     /translation="MGCVLGNKVSGGDSPVSLFRITENSSFALWNGGDFGFEGGVLCS
                     LILVGCLAYFSLESVRVSVRY"
     gene            296..1288
                     /gene="plsX"
                     /locus_tag="VBC28_000877"
     CDS             296..1288
                     /gene="plsX"
                     /locus_tag="VBC28_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000716823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEO6814878.1"
                     /translation="MKKIAIDAMGGDYAPKAIVEGVNQAISDFSDIEVQLYGDQKKIE
                     KYLTVTERVSIIHTEEKINSDDEPAKAVRRKKQSSMVLGAKAVKDGVAQAFISAGNTG
                     ALLAAGLFVVGRIKGVDRPGLMSTMPTLDGVGFDMLDLGANAENTASHLHQYAILGSF
                     YAKNVRGIEVPRVGLLNNGTEETKGDSLHKEAYELLAAEPSINFIGNIEARDLMSSVA
                     DVVVTDGFTGNAVLKTMEGTAMSIMGSLKSSIKSGGVKAKLGALLLKDSLYQLKDSMD
                     YSSAGGAVLFGLKAPIVKCHGSSDSKAVYSTLKQVRTMLETQVVDQLVDAFTDE"
     gene            1299..1538
                     /locus_tag="VBC28_000878"
     CDS             1299..1538
                     /locus_tag="VBC28_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085641.1"
                     /note="carries the fatty acid chain in fatty acid
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEO6814879.1"
                     /translation="MSRDEVFEKMLELLRQQLGDPQLDITPESSLHDDLAIDSIALTE
                     FIINLEDVFHLEIPDEAVEHMSSVQQLLDYIIEHK"
     gene            1662..2366
                     /locus_tag="VBC28_000879"
     CDS             1662..2366
                     /locus_tag="VBC28_000879"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEO6814880.1"
                     /translation="MTNQLIYTGKAKDIYSTKDENVIRTVYKDQATMLNGARKETIDG
                     KGALNNQISSLIFEKLNMAGVVTHYIEQISKNEQLNKKVDIIPLEVVLRNVTAGSFSK
                     RFGVEEGHVLETPIVEFYYKNDNLNDPFINDEHVKFLGIVNDEEIAYLKGETRHINEL
                     LKDWFAQIGLNLIDFKLEFGFDKDGKIILADEFSPDNCRLWDADGNHMDKDVFRRDLG
                     SLTDVYQVVLEKLIAL"
     gene            2489..6214
                     /locus_tag="VBC28_000880"
     CDS             2489..6214
                     /locus_tag="VBC28_000880"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HEO6814881.1"
                     /translation="MNKRIFVEKKADFGIKSASLVKELTHNLQLASLKDLRIVQVYDV
                     FNLAEDLLARAEKHIFSEQVTDRLLTEAEITAELDKVAFFAIEALPGQFDQRAASSQE
                     ALLLLGSDSQVKVNTAQLYLVNKDIAEAELEAVKNYLLNPVDSRFKDITLPLEVQAFS
                     VSDKTISNLDFFETYQADDFAAYKAEQGLAMEVDDLLFIQDYFKSIGHVPTETELKVL
                     DTYWSDHCRHTTFETELKNIDFSASKFQKQLQATYDKYIAMRDELGRSEKPQTLMDMA
                     TIFGRYERANGRLDDMEVSDEINACSVEIEVDVDGVKEPWLLMFKNETHNHPTEIEPF
                     GGAATCIGGAIRDPLSGRSYVYQAMRISGAGDITTPIAETRAGKLPQQVISKTAAHGY
                     SSYGNQIGLATTYVREYFHPGFVAKRMELGAVVGAAPKENVVREKPEAGDVVVLLGGK
                     TGRDGVGGATGSSKVQTVESVETAGAEVQKGNAIEERKIQRLFCDGNVTRLIKKSNDF
                     GAGGVCVAIGELADGLEIDLDKVPLKYQGLNGTEIAISESQERMSVVVGPSDVDAFIA
                     ACNKENIDAVVVATVTEKPNLVMTWNGETIVDLERCFLDTNGVRVVVDAKVVDKDLTV
                     PEARTTSAETLEADMLKVLSDLNHASQKGLQTIFDSSVGRSTVNHPIGGRYQITPTES
                     SVQKLPVQYGVTTTASVMAQGYNPYIAEWSPYHGAAYAVIEATARLVATGADWSRARF
                     SYQEYFERMDKQAERFGQPVSALLGSIEAQIQFGLPSIGGKDSMSGTFEELTVPPTLV
                     AFGVTTADSRKVLSPEFKAAGENIYYIPGQAISEDIDFDLIKANFSQFEAIQAQHKIT
                     AASAVKYGGVLESLALMTFGNRIGASVEIAELDSSLTAQLGGFVFTSVEEIADVVKIG
                     QTQADFTVTVNGNDLAGASLLSAFEGKLEEVYPTEFEQVDAIEEVPAVVSDVVIKAKE
                     IIEKPVVYIPVFPGTNSEYDSAKAFEQVGASVNLVPFVTLNEAAIAESVDTMVANIAK
                     ANIIFFAGGFSAADEPDGSAKFIVNILLNEKVRAAIDSFIEKGGLIIGICNGFQALVK
                     SGLLPYGNFEEAGETSPTLFYNDANQHVAKMVETRIANTNSPWLAGVEVGDIHVIPVS
                     HGEGKFVVSASEFAELRDNGQIWSQYVDFDGQPSMDSKYNPNGSVNAIEGITSKNGQI
                     IGKMGHSERWEDGLFQNIPGNKDQKLFESAVKYFTGK"
     gene            6626..8080
                     /locus_tag="VBC28_000881"
     CDS             6626..8080
                     /locus_tag="VBC28_000881"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEO6814882.1"
                     /translation="MTYEVKSLNEECGVFGIWGYPQAAQVTYFGLHSLQHRGQEGAGI
                     ISNDNGKLYGYRNVGLLSEVFKNQSELDNLTGNAAIGHVRYATAGSADIRNIQPFLYK
                     FHDGQFALCHNGNLTNAISSRKELEKQGAIFNASSDTEILMHLIRRSHNPSFMGKVKE
                     ALSTVKGGFAYLLMTEDKLIAALDPNAFRPLSIGQMQNGAWVISSETCAFEVVGAKWV
                     RDVEPGEVILIDDSGIQCGRYTDETQLAICSMEYVYFARPDSTIHGVNVHTARKNMGK
                     RLAQEFKQDADIVIGVPNSSLSAAMGFAEESGLPNEMGLVKNQYTQRTFIQPTQELRE
                     QGVRMKLSAVSGVVKGKRVVMIDDSIVRGTTSRRIVGLLREAGATEVHVAIASPELKY
                     PCFYGIDIQTRRELISANHAVDEVCDIIGADSLTYLSIDGLIKSIGLETKAPNGGLCV
                     AYFDGHYPTPLYDYEEEYLRSLEEKTSFYIQKVK"
     gene            8108..9130
                     /locus_tag="VBC28_000882"
     CDS             8108..9130
                     /locus_tag="VBC28_000882"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEO6814883.1"
                     /translation="MSEKNAYAQSGVDVEAGYEVVERIKKHVARTERAGVMGALGGFG
                     GMFDLSQTGVKEPVLISGTDGVGTKLMLAIKYDKHDTIGQDCVAMCVNDIIAAGAEPL
                     YFLDYVATGKNEPAKLEQVVAGVAEGCVQASAALIGGETAEMPGMYGEDDYDLAGFAV
                     GVAEKSQIIDGSKVKEGDILLGLASSGIHSNGYSLVRRVFADYTGDEVLPELEGKQLK
                     DVLLEPTRIYVKAALPLIKEELVNGIAHITGGGFIENVPRMFADDLAAEIDEDKVPVL
                     PIFKALEKYGDIKHEEMFEIFNMGVGLMLDVNPENVDRVKELLDEPVYEIGRIIKKAD
                     DSVVIK"
     gene            9298..9846
                     /gene="purN"
                     /locus_tag="VBC28_000883"
     CDS             9298..9846
                     /gene="purN"
                     /locus_tag="VBC28_000883"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEO6814884.1"
                     /translation="MKIAVFASGNGSNFQVIAEQFQVSFVFSDHRDAYVLERAQNLAI
                     PSFAFELKEFENKAAYEQAVVDLLDKHEIDLVCLAGYMKIVGETLLSAYEGRIINIHP
                     TYLPEFPGAHGIKDAWEAGVDQSGVTIHWVDSGVDTGQVIQQVHVPRLADDSLESFET
                     RIHETEYQLYPAVLDSLGIKRK"
     gene            9869..10621
                     /locus_tag="VBC28_000884"
     CDS             9869..10621
                     /locus_tag="VBC28_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO6814885.1"
                     /translation="MKLVKNLEIVESIFGDWDETIIWSCVQGIMGEVFVDSLDQPKSS
                     LAKLGRKSSFGFLAGQPTLFLLEVCSGEDIILVPQHKGWSDLIESTYGQNAHSFKRYA
                     TKKDTLFERSRLEKFVTQLPNGFELRAIDEKVYNSCLEKEWSQDLVANYATYQYYKKQ
                     GIGYVVYYQGNIIAGASSYSTYKNGIEIEVDTHPDFRRRGLATIVAAQLILTCLDKGI
                     YPSWDAHTRTSLNLSEKLGYEFSHEYIAYEID"
     gene            10641..12188
                     /gene="purH"
                     /locus_tag="VBC28_000885"
     CDS             10641..12188
                     /gene="purH"
                     /locus_tag="VBC28_000885"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006146083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEO6814886.1"
                     /translation="MTKRALISVSDKSGIIDFAKELKNLGWDIISTGGTKVALDDAGV
                     ETIAIDDVTGFPEMMDGRVKTLHPNIHGGLLARRDADSHLQAAKDNNIELIDLVVVNL
                     YPFKETILRPDVTYDLAVENIDIGGPSMLRSAAKNHASVTVVVDSADYATVLGELADA
                     SQTTFKTRQRLAAKAFRHTAAYDALIAEYFTAQVGEAKPEKLTITYDLKQAMRYGENP
                     QQDADFYQKALPTDYSIASAKQLNGKELSFNNIRDADAAIRIIRDFKDSPTVVALKHM
                     NPCGIGQADDIETAWDYAYEADPVSIFGGIVVLNREVDAATAEKMHPIFLEIIIAPSY
                     SEEALAILTNKKKNLRILELPFDAQAASEVEAEYTGVVGGLLVQNQDVVAENPSDWQV
                     VTDRQPTEQEATALEFAWKAIKYVKSNGIIITNDHMTLGLGAGQTNRVGSVKIAIEQA
                     KDHLDGAVLASDAFFPFADNIEEIAAAGIKAIIQPGGSVRDQESIDAANKHGLTMIFT
                     GVRHFRH"
     gene            12381..13280
                     /locus_tag="VBC28_000886"
     CDS             12381..13280
                     /locus_tag="VBC28_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001045916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEO6814887.1"
                     /translation="MNKWLVKASSLVVLGGMVLSAGSRVLADTYVRPIDNGRITTGFN
                     GYPGHCGVDYAVPTGTIIRAVADGTVKFAGAGANFSWMTDLAGNCVMIQHADGMHSGY
                     AHMSRVVARTGEKVKQGDIIGYVGATGMATGPHLHFEFLPANPNFQNGFHGRINPTSL
                     IANVATFSGKTQASAPSIKPLQSAPVQNQSSKLKVYRVDELQKVNGVWLVKNNTLTPT
                     GFDWNDNGIPASEIDEVDANGNLTADQVLQKGGYFIFNPKTLKTVEKPIQGTAGLTWA
                     KTRFANGSSVWLRVDNSQELLYK"
     gene            13427..14731
                     /locus_tag="VBC28_000887"
     CDS             13427..14731
                     /locus_tag="VBC28_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000783422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEO6814888.1"
                     /translation="MKMNKKVLLTSTMAASLLSVASVQAQETDTTWTARTVSEVKADL
                     VKQDNKSSYTVKYGDTLSVISEAMSIDMNVLAKINNIADINLIYPETTLTVTYDQKSH
                     TATSMKIETPATNAAGQTTATVDLKTNQVSVADQKVSLNTISEGMTPEAATTIVSPMK
                     TYSSAPALKSKEVLAQEQAVSQAAANEQVSPAPVKSITSEVPAAKEEVKPTQTSVSQS
                     TTVSPASVAAETPAPVAKVAPVRTVAAPRVASVKVVTPKVETGASPEHVSAPAVPVTT
                     TSPATDSKLQATEVKSVPVAQKAPTATPVAQPASTTNAVAAHPENAGLQPHVAAYKEK
                     VASTYGVNEFSTYRAGDPGDHGKGLAVDFIVGTNQALGNKVAQYSTQNMAANNISYVI
                     WQQKFYSNTNSIYGPANTWNAMPDRGGVTANHYDHVHVSFNK"
     gene            14978..15676
                     /locus_tag="VBC28_000888"
     CDS             14978..15676
                     /locus_tag="VBC28_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEO6814889.1"
                     /translation="MPHLSKEAFKKQIKNGIIVSCQALPGEPLYTESGGVMPLLALAA
                     QEAGAVGIRANSVRDIKEIQEVTNLPIIGIIKREYPPQEPFITATMTEVDQLASLDIA
                     VIALDCTLRERHDGLSVVEFIQKIKRKYPEQLLMADISTFEEGKNAFEAGVDFVGTTL
                     SGYTDYSRQEEGPDIELLNKLCQAGIDVIAEGKIHTPKQANEINHIGVAGIVVGGAIT
                     RPKEIAERFISGLS"
     gene            15723..17039
                     /locus_tag="VBC28_000889"
     CDS             15723..17039
                     /locus_tag="VBC28_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEO6814890.1"
                     /translation="MSIKKSVIGFCLGAAALSMFACVDSSQSVMAAEKDKVEITWWAF
                     PTFTQEKAKDGVGTYEKKVIKAFEKKNPNIKVKLETIDFTSGPEKITTAIEAGTAPDV
                     LFDAPGRIIQYGKNGKLADLNDLFTDQFIKDVNNKNIIQASKSGDKAYMYPISSAPFY
                     MAFNKKMLKDAGVLKLVKEGWTTSDFEKVLKALKNKGYTPGSFFANGQGGDQGPRAFF
                     ANLYSAPITDKEVTKYTTDTKNSVKSMKKIVEWIKKGYLMNGSQYDGSADIQNFANGQ
                     TAFTILWAPAQPKTQAKLLESSKVDYLEVPFPSEDGKPDLEYLVNGFAVFNNKDENKV
                     KASKKFITFIADDKKWGPKDVIRTGAFPVRTSFGDLYKGDKRMMKISKWTQYYSPYYN
                     TIDGFSEMRTLWFPMVQSVSNGDEKPADALKDFTQKANDTIKKAAK"
     gene            17127..18014
                     /locus_tag="VBC28_000890"
     CDS             17127..18014
                     /locus_tag="VBC28_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003041053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEO6814891.1"
                     /translation="MRTNKLKMRETMIAYAFLAPILLFFLIFVFAPMVMGFVTSFFNY
                     SMTQFTFIGLANYNRMFHDSIFMKSLINTVIIVIGSVPVVVFFSLFVAANTYEKNVFS
                     RSFYRCVFFLPVVTGSVAVTVVWKWIYDPMSGILNYILKSGHVIEQNISWLGDKHWAL
                     LAIIIILLTTSVGQPIILYIAAMGNIDNSLCEAARVDGANEMQVFWQIKWPSLLPTTL
                     YIAVITTINSFQCFALIQLLTSGGPNYSTSTLMYYLYEKAFKLSEYGYANTMGVFLAV
                     MIALISFAQFKILGNDVEY"
     gene            18024..18854
                     /locus_tag="VBC28_000891"
     CDS             18024..18854
                     /locus_tag="VBC28_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEO6814892.1"
                     /translation="MKKKTFSAYNFLTALILCLLTVLFIFPFYWIMTGAFKSQPDTII
                     IPPQWWPKAPTLENFKALTVQNPALRWLWNSVFISIMTMFLVCCTSSMAGYVLAKKRF
                     YGQKILFSLFIAAMALPKQVVLVPLVRIINFMGIHDTLWAVILPLVGWPFGVFLMKQF
                     SENIPTELLESAKIDGCGEIRTFINVAFPIVKPGFAALAIFTFINTWNDYFMQLVMLT
                     SRNNLTISLGVATMQAEMATNYGLIMAGAALAAVPIVTVFLVFQKSFTQGITMGAVKG
                     "
     gene            18867..19310
                     /locus_tag="VBC28_000892"
     CDS             18867..19310
                     /locus_tag="VBC28_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HEO6814893.1"
                     /translation="MIYDHLLNLTHYKDINPNLDLAIDYLLSHDLRNLDIGTYHISPE
                     VILMVQSNQLSESFDHIFEYHKKYLDIHYVIEGHEVIKLGKGDKVEVEEYLGDIGFIK
                     CSEETSFDLRDNYIAFFFPEEAHQPNGMGSLGNYVKKGVLKVLMT"
     gene            19330..19992
                     /locus_tag="VBC28_000893"
     CDS             19330..19992
                     /locus_tag="VBC28_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HEO6814894.1"
                     /translation="MKKANQLIAAIFDVNNPFMQGCNVVFDLALLNLLFMITCLPLVT
                     IGAAKISLYRTLWQKLEGDQTNLLILYIKHLKKEWFQGMLLGLVELSILVVIIFDLTI
                     LHYQIGFIVSFLKITCYAFLLLTVMTSIYLFPMAARYEMSLLDTVKKSFIMACLNLKW
                     TGVLMFLLIMTWFIMVQSSLLFMLTVSAIFIFAYTAFAYFKIIILQKQFAYFSKQQGD
                     YQ"
     gene            19989..20906
                     /locus_tag="VBC28_000894"
     CDS             19989..20906
                     /locus_tag="VBC28_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000654097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEO6814895.1"
                     /translation="MKDLQKYQGIIPAFYACYDDKGDICPERVKALTNYFIDKGVQGL
                     YVNGSSGECIYQSVADRKLVLENVMSVAKGKLTVIAHVACNNTKDSVELAMHAEAIGV
                     DAIAAIPPIYFRLPEYAIADYWNTISQAAPQTDFIIYNIPQLAGVALTSDLYRKMLQN
                     PQVIGVKNSSMPVQDIQNFVAIGGENHIVFNGPDEQFLGGRLMGAAAGIGGTYGVMPE
                     LYLTLNQLIVDKDLEKARELQFTINDIITKLCSGHGNMYAVIKAVLEINEQLTIGSVR
                     LPLASVTEEDKPIIKEAAEMIRHAKKQFC"
     gene            20923..21804
                     /locus_tag="VBC28_000895"
     CDS             20923..21804
                     /locus_tag="VBC28_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEO6814896.1"
                     /translation="MTRTVAIDIGGTMIKHGIVDNLGCIVEASELATEAYKGGPGILQ
                     KVCQIIDNYLAEGSIDGIAISSAGMVDPDEGCIFYSGPQIPNYAGTQFKKVLEDTYQV
                     RTEIENDVNCAGLAEAVSGSAKDSSIALCLTIGTGIGGCLIIDKTVFHGFSNSACEVG
                     YMHLSDGDFQDLASTTALIADVAKAHGDEISRWDGRRIFQEAKKGNEKCIASIDRMIN
                     YLGQGIANMVYVVNPEKVVLGGGIMAQKDYLQDKLSESLKRNLVTSLAEKTAIVFAQH
                     ENQAGMLGAYYHFKNRG"
     gene            21812..22789
                     /locus_tag="VBC28_000896"
     CDS             21812..22789
                     /locus_tag="VBC28_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEO6814897.1"
                     /translation="MIETMSLDDMREYLGQDQIPEDFDDFWKKQTMKYQGNIEYRLDK
                     KDFNITFAQAYDLHFKGSNNSIVYAKCLFPKTNKPYPVVFYFHGYQNQSPDWSDQLNY
                     VAAGYGVVSMDVRGQAGQSQDKGHFDGITVKGQIVRGMISGPNHLFYKDIYLDVFQLI
                     DIIATLESVDSNQLYSYGWSQGGALALIAAALNPKIVKTVAVYPFLSDFRRVLDLGGV
                     SEPYDELFRYFKYSDPFHKTENNVLKTLAYIDVKNFAHRISCPVVLLTALKDDICPPS
                     TQFAIFNRLTSTKKHLLLPDYGHDPMTVQVKDHIFDQLTGSQFTKQKIE"
     gene            complement(22812..23615)
                     /locus_tag="VBC28_000897"
     CDS             complement(22812..23615)
                     /locus_tag="VBC28_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000158185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEO6814898.1"
                     /translation="MTIYDQIESALDLMTDLEREIACYFMGQPISKDALASTIVTKQL
                     HISQAALTRFAKKCGFKGYREFVFEYLKSHETISQQLYGLQNDNTKKVFMNYQEMISK
                     SADIIDEEQLLEVSHMIEQADRVYFYGKGSSSLVAKEFKIRLMRLGVICEALDDTDSF
                     SWTNSIVNDRCLVIAFSLSGNTNSVIGALKIASCHGAKTVLFTKQPHTIDYAFDKIIQ
                     VASARHLDYGNRISPQIPMLIMVDIIYAQFLDINKIEKERIFRETIIQR"
     gene            23897..25162
                     /gene="purD"
                     /locus_tag="VBC28_000898"
     CDS             23897..25162
                     /gene="purD"
                     /locus_tag="VBC28_000898"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEO6814899.1"
                     /translation="MKLLVVGSGGREHAIAKKLLASKDVDQVFVAPGNDGMTLDGLDL
                     VNIGISEHSRLIDFVKENEIAWTLIGPDDALAAGIVDGFNSAGLRAFGPTKAAAELEW
                     SKDFAKEIMVKYNVPTAAYGTFSDFEKAKAYIEEQGAPIVVKADGLALGKGVVVAETV
                     EQAVEAAQEMLLDNKFGDSGARVVIEEFLDGEEFSLFAFANGDKFYIMPTAQDHKRAY
                     DGDKGLNTGGMGAYAPVPHLPQSVVDTAVETIVKPVLEGMIAEGRPYLGVLYAGLILT
                     ADGPKVIEFNSRFGDPETQIILPRLTSDFAQNIDDIMMGIEPYITWQKDGVTLGVVVA
                     SEGYPLDYEKGVPLPEKTDGDIITYYAGAKFAENSKALLSNGGRVYMLVTTEDSVKAG
                     QDKIYTQLAQQDTTGLFYRNDIGSKAIKE"
     gene            25443..25931
                     /gene="purE"
                     /locus_tag="VBC28_000899"
     CDS             25443..25931
                     /gene="purE"
                     /locus_tag="VBC28_000899"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEO6814900.1"
                     /translation="MQPIISIIMGSKSDWTTMQKTAEVLDNFGIAYEKKVVSAHRTPD
                     LMFKHAEEARGRGIKIIIAGAGGAAHLPGMVAAKTTLPVIGVPVKSRALSGLDSLYSI
                     VQMPGGVPVATMAIGEAGATNAALTALRILSIEDQNLADALAHFHEEQGKIAEESSNE
                     LI"
     gene            25918..27009
                     /gene="purK"
                     /locus_tag="VBC28_000900"
     CDS             25918..27009
                     /gene="purK"
                     /locus_tag="VBC28_000900"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001084009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEO6814901.1"
                     /translation="MNSFKTIGIIGGGQLGQMMAIAAIYMGHKVITLDPASDCPASRV
                     SEVIVAPYDDVEALGTLAARCDVLTYEFENVDADGLDAVVSAGQLPQGTDLLRISQNR
                     IFEKDFLANKAGVTVAPYKVVTSSLDLEGLDLTKTYVLKTATGGYDGHGQKVIRSAED
                     LPEAQQLANSAQCVLEEFVNFDLEISVIVSGNGQDVTVFPVQENIHRNNILSKTIVPA
                     RISDQLADKAKEMAVQIAKKLQLSGTLCVEMFATADDIIVNEIAPRPHNSGHYSIEAC
                     DFSQFDTHILGVLGAPLPPIKLHAPAVMFNVLGQHVQQAIDHVAQNPSAHLHMYGKLE
                     AKHNRKMGHVTVFSDVPDEVEEFEERMDF"
     gene            27063..>27447
                     /locus_tag="VBC28_000901"
     CDS             27063..>27447
                     /locus_tag="VBC28_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6814902.1"
                     /translation="MNRIINRDILPRISKISKNNKEKDLLSIAYITWLIFIIFALGVV
                     TVNDLKPMFNQLIVNLLNIYYYMEAFILGMDSYLQYNLPYSFDFWSIFVEAINLFVKV
                     FLIAFIPSVIRKVLKKESFFNEVVIL"
BASE COUNT         8326 a         4890 c         6005 g         8226 t
ORIGIN      
        1 atactacatg gaattttact atgggatgtg tattaggtaa taaggttagt gggggggatt
       61 ctcctgtttc tctttttaga attactgaaa attctagctt tgctctttgg aatggtgggg
      121 attttggttt tgaaggggga gtactatgtt ctttgatatt agtaggttgt ttagcatact
      181 tttctttgga aagcgtgaga gtatctgtta gatattagct attattgcct tcttgtgaaa
      241 aaaataaaga aacgtgctat aatatagcca ctaagttttt atgaggagtt cattcatgaa
      301 gaaaattgct atagatgcta tgggtggaga ttacgctccc aaagctattg tagagggtgt
      361 taatcaagct atttcagatt ttagtgatat tgaagtacaa ctctatggcg atcagaaaaa
      421 aattgagaaa tatttaacag taacggagcg agttagtatt attcatactg aagaaaaaat
      481 taattctgat gatgagcctg ctaaagctgt tcgtcgtaaa aaacagtctt cgatggtctt
      541 aggagctaag gcagtcaaag atggtgttgc acaggccttt atttctgcag ggaatacagg
      601 agctttatta gctgctggtt tgtttgtagt aggacgtata aaaggtgttg atcgaccagg
      661 cttaatgtca actatgccta cgttagatgg tgtgggtttt gatatgcttg atttaggagc
      721 taatgcagaa aacacagctt ctcatcttca tcaatatgct attttagggt cgttttatgc
      781 aaaaaatgtc cgtggtattg aagttcctcg tgtaggtctc ttaaataacg gaacggaaga
      841 aacaaaaggc gattctcttc ataaagaagc ttatgaactg ttagcagcag agccttctat
      901 taattttatt ggaaatattg aagcacgtga tttgatgtct agtgtagcag atgttgttgt
      961 cactgatggt tttactggaa atgctgttct aaagacaatg gaaggtacag ctatgagtat
     1021 tatgggaagc ctaaaatctt caattaaatc aggtggcgtg aaagctaaat taggagcact
     1081 tctattaaaa gatagcctat atcagctaaa agatagtatg gactattcaa gcgcgggtgg
     1141 agcggttctt tttggtttaa aagctccaat agtaaaatgc catggatcaa gtgattctaa
     1201 agctgtttat tctactttga agcaagttcg tacaatgttg gaaactcaag tagttgatca
     1261 attagttgat gcctttacag atgaatagga gtgaactcat gtcaagagat gaagtttttg
     1321 aaaagatgct ggaattatta aggcagcaac ttggtgaccc acagttagat atcacaccag
     1381 agtcatcgtt acatgatgat ttagcaattg actctatcgc tttaacagag tttattatca
     1441 atttggaaga tgtgtttcat ctagagattc cagatgaggc tgtcgaacac atgtcaagcg
     1501 tccaacaact attagattat atcattgaac ataagtaata ataaaaagga agaaaatacg
     1561 aacgtttctt cttttttttg attttaaata aagtttttgt tgtattagat aatgttgtgt
     1621 gttagaataa ctcttaaaat aagagaaagg cgaataatca agtgactaac cagcttattt
     1681 atacaggaaa agcgaaagat atttatagta caaaagatga aaatgtgatt agaacggtct
     1741 ataaagacca agctactatg ttgaatggag ctcgtaagga gactattgat ggcaagggag
     1801 cactaaataa tcagatttca tctcttattt ttgagaaatt aaatatggct ggtgtggtga
     1861 cacattatat tgagcaaatt tcaaaaaacg aacaattgaa taaaaaagtt gatattattc
     1921 cacttgaggt ggtgctacgg aatgtgacag caggctcatt ttcaaaacgt tttggagtgg
     1981 aagaaggaca cgttcttgaa acgccaatcg tggaatttta ctacaaaaat gataatctga
     2041 atgatccttt tatcaatgat gagcatgtga agtttttggg tattgttaat gatgaggaga
     2101 ttgcttatct gaaaggagag actcgccata ttaatgaact cttgaaagat tggtttgctc
     2161 agattgggct taacttgatt gacttcaagt tggagttcgg atttgacaag gatggcaaaa
     2221 tcatcttggc tgatgagttc tcacctgaca actgccgtct ctgggatgct gatggtaatc
     2281 atatggacaa ggatgttttt cgtagggatc ttggtagctt gaccgatgtc tatcaagttg
     2341 tacttgagaa gttgatagca ttgtaattag aagaggaagg aattgaatcc gttcgggaaa
     2401 ctcagaaaaa agattgggct tactgaaatc tcaatttcag acgtcctcct attttcgagt
     2461 cgctcccttg tcggacttgg tatcttatat gaataaacgt atttttgttg agaaaaaggc
     2521 tgactttggc attaaatcgg ctagtcttgt gaaagagttg acgcataatc tacaactggc
     2581 ttctttgaag gatttgcgta tcgtgcaggt ctatgatgtc ttcaatttgg ctgaggattt
     2641 gctggcgcgt gctgagaagc atattttctc tgagcaggtg acagaccgtc ttttgacaga
     2701 agctgaaatc actgcggagc ttgataaggt tgccttcttt gccattgagg cgcttcctgg
     2761 tcaatttgac caacgtgctg ctagttcgca agaagctttg ctattgcttg gaagtgacag
     2821 tcaggtcaag gtcaatacag ctcagcttta cttggtcaat aaggatattg cagaagcaga
     2881 gcttgaagcc gttaagaact atcttttgaa tcccgttgat tcacgtttca aggatattac
     2941 cttgccactt gaagtgcagg ctttctctgt gtctgataag acaatctcta atcttgattt
     3001 cttcgaaact tatcaggctg acgattttgc agcctataag gcagagcagg gattggctat
     3061 ggaggtcgat gaccttctct tcatccaaga ttatttcaaa tcaatcgggc atgtgccaac
     3121 tgagactgag ttgaaagttt tggatacgta ttggtcagac cactgccgtc acacaacctt
     3181 tgaaactgaa ttgaaaaaca ttgatttttc ggcctctaaa ttccaaaaac aattgcaggc
     3241 gacttatgac aaatacattg ccatgcgtga tgagcttggt cgttctgaaa agccgcagac
     3301 acttatggat atggcgacta tctttggtcg ttatgagcgt gccaatggtc gtctggacga
     3361 tatggaagtc tcagatgaaa tcaatgcctg ctcagttgaa attgaagtag atgttgatgg
     3421 tgtgaaagag ccttggctcc tcatgtttaa gaacgagact cacaaccacc caacagaaat
     3481 tgagccattt ggtggagcgg cgacttgtat cggtggtgcc attcgtgacc cattgtcagg
     3541 acgttcatac gtttatcagg ctatgcgtat ttcaggcgca ggagatatca cgactccgat
     3601 tgcggaaaca cgtgctggta aattgccaca acaggttatt tctaagactg cggcgcacgg
     3661 ctattcttca tatggtaacc aaattgggct tgcgacaact tatgtgcgtg agtacttcca
     3721 ccctggcttc gtagctaaac gcatggagct tggagctgtg gttggtgctg cacctaagga
     3781 aaatgtggtt cgtgaaaaac cagaggcagg cgatgtggtt gtcttgctcg gtggtaaaac
     3841 aggtcgtgac ggtgtcggcg gtgcgacagg ttcatctaag gttcaaacgg ttgaatctgt
     3901 ggaaacagct ggcgcagagg tacaaaaagg gaatgccatc gaagaacgta agattcaacg
     3961 ccttttctgt gatggtaatg tcactcgcct aatcaagaaa tcaaatgact tcggtgcagg
     4021 tggtgtctgt gttgccatcg gtgaattagc tgatggtctt gaaatcgatt tggacaaggt
     4081 gccacttaaa taccaaggtc ttaatggtac tgaaattgct atctcagaat ctcaagaaag
     4141 gatgtcagtt gttgttggtc caagtgatgt ggatgccttc atcgcagcct gtaacaagga
     4201 aaatatcgat gcagttgttg ttgcgacagt tactgaaaaa ccaaatcttg tcatgacttg
     4261 gaatggtgaa acaattgttg atttggaacg ctgtttcctt gataccaacg gtgttcgtgt
     4321 cgtagttgat gccaaagtcg ttgacaagga cttaacagtt ccagaagcgc gcacaacatc
     4381 agcagagaca cttgaagcag atatgcttaa ggtcttgtct gacctcaatc acgctagtca
     4441 aaaaggtctt caaactatct ttgactcatc tgttggtcgt tcaaccgtta accacccaat
     4501 cggtggtcgt taccaaatca caccgacaga aagttctgtt caaaaattgc cagttcaata
     4561 tggtgtgaca acaacagcat ctgttatggc tcaaggttac aatccttata ttgcagagtg
     4621 gtcaccttat cacggtgctg cctatgctgt tattgaagcg acagctcgct tggtagcaac
     4681 gggtgctgac tggtctcgtg cacgtttctc ttaccaagag tatttcgagc gtatggataa
     4741 acaggcagag cgttttggtc agccagtatc agctcttctt ggttctattg aggctcagat
     4801 tcaatttggt ttgccatcaa tcggcggtaa ggactctatg tctggtactt tcgaagaatt
     4861 gacagtacca ccaaccttgg tagctttcgg tgtgacaaca gcggacagcc gcaaggttct
     4921 ctctcctgag tttaaagcgg ctggtgagaa tatctactac attccaggtc aagctatttc
     4981 agaagatatt gattttgacc ttatcaaggc taactttagc cagtttgagg ctattcaagc
     5041 tcaacataag attaccgctg cctcagccgt taaatacggt ggtgtcctag aaagtcttgc
     5101 tctcatgact tttggtaacc gtatcggtgc ttctgttgaa attgcagagc ttgacagcag
     5161 cttgacagct caactcggag gctttgtctt tacatcagtt gaggaaattg ctgacgttgt
     5221 gaagatcggt caaactcagg cagactttac agtcactgtc aatggaaatg acctagctgg
     5281 tgcaagcctt ctatcagcct tcgaaggtaa attagaagaa gtttacccaa cagagtttga
     5341 acaggtagat gctattgagg aagttcctgc tgtggtatca gatgttgtta tcaaggctaa
     5401 ggaaattatt gaaaaaccag tagtttacat cccagtcttt cctggtacta actcagagta
     5461 cgattcagct aaggcctttg aacaggttgg agctagcgtc aacttggtgc catttgtaac
     5521 cttgaatgag gctgctattg ctgagtcagt tgatacgatg gttgctaata ttgctaaggc
     5581 aaatatcatc ttctttgctg gaggtttctc agcagcggat gaaccagatg gctctgctaa
     5641 gtttatcgtc aatatcttgc ttaacgagaa ggttcgcgca gctattgaca gcttcatcga
     5701 aaaaggtggc cttatcatcg gtatctgtaa tggtttccaa gcccttgtta aatcaggtct
     5761 tcttccatac ggaaacttcg aggaagctgg tgagacaagt ccaaccctct tctataacga
     5821 tgctaaccag cacgttgcaa agatggttga gactcgtatc gcaaatacca attcaccttg
     5881 gttggcagga gttgaggtcg gcgatattca tgtcattcca gtttcacacg gtgaaggtaa
     5941 atttgttgtc agcgcttctg aatttgcaga gctaagagac aatggtcaaa tctggagcca
     6001 atatgtggac tttgacggac aaccatctat ggattctaaa tacaatccaa acggctctgt
     6061 caatgccatc gaagggatta ccagcaagaa tggtcaaatc atcggtaaga tggggcactc
     6121 agaacgctgg gaagacggac tcttccaaaa tatccctgga aacaaagacc aaaaactctt
     6181 tgaaagtgct gtaaaatact ttacagggaa gtgagagtgg gacaaaaatc ggtaattcca
     6241 aaggaattcg attttgtcgt ccccctccgc acagttgagt ggggctgtaa gagttgattt
     6301 atcagcgaat tagagcccac tcaaccccta cgttttgcga actagattat aaaaaatatg
     6361 gaggcttagg acttttgtcc tagctttagc cctcgccttg aattagtgaa ggcgaggtta
     6421 gatagaaatt cgctatcttt tgaacgaagc gagcttaacc gtatctttcc gccgtattgg
     6481 tatttgggag tcattgagac taaggttcaa tggctagctt gatagatttt ctattttaat
     6541 gttggcattg atgacgacag atccaaaatt gagaaaatca tcaaagctac ttaagaagag
     6601 atacaaaaag aattaggaaa aaagaatgac atacgaagta aaatctctaa atgaagaatg
     6661 tggagtcttt ggtatctggg ggtatcctca ggcagctcaa gtcacttact ttgggcttca
     6721 tagccttcaa catcgcggtc aagaaggagc tggtattatt tcaaatgata atgggaaact
     6781 ctatggttat cgaaatgttg ggctcctttc tgaagttttt aagaatcaat ctgaattaga
     6841 taatttaact gggaatgcgg ctattggaca tgttcggtat gctactgcag gttctgcaga
     6901 tattcgcaat attcagcctt ttctttataa atttcatgat gggcaatttg ctttatgtca
     6961 taatggtaat ttgacaaatg ctatttcctc aaggaaagaa ttagaaaagc aaggtgcaat
     7021 tttcaatgcc tcctcagata ctgaaatttt aatgcatttg attcgtcgaa gccataaccc
     7081 aagttttatg gggaaggtaa aagaagcttt aagcactgta aagggaggtt tcgcctatct
     7141 actgatgaca gaagataaat taattgctgc tcttgaccct aatgcctttc gtcctttgtc
     7201 aattggacaa atgcaaaatg gtgcctgggt tatttccagt gagacctgtg cttttgaggt
     7261 ggtaggcgca aaatgggtta gagatgttga acctggtgaa gttattctta tagacgatag
     7321 tggtattcaa tgtggtcgtt atactgatga aacgcaactg gcaatttgtt cgatggaata
     7381 tgtctatttt gcaaggccgg attcaactat acatggtgtt aatgttcata cggctcgaaa
     7441 aaatatggga aagcgtcttg cacaagaatt taaacaggat gctgatattg taattggtgt
     7501 cccaaattcg tccttatcgg ctgctatggg ctttgctgaa gaatccggat taccaaatga
     7561 gatgggcctt gtaaaaaatc agtatacgca gcgaaccttt attcaaccga cacaagaatt
     7621 aagggaacaa ggtgttcgaa tgaaattatc agcggtatct ggtgttgtca aaggaaagcg
     7681 cgttgttatg attgatgact caattgtaag aggaacgact tctagaagga ttgtaggatt
     7741 attaagagaa gcaggagcta ctgaagtaca tgttgctata gctagtccag aattaaagta
     7801 tccttgtttt tatggaattg atattcaaac aagacgagaa ttaatttcag caaatcacgc
     7861 tgttgacgaa gtgtgtgata ttattggcgc agatagtcta acttatctat caatcgatgg
     7921 gttaattaaa tcaattggac tcgaaacaaa agcaccaaat ggtggtttat gcgtagctta
     7981 ctttgatgga cattatccaa caccgcttta tgattatgaa gaagaatacc tcagaagttt
     8041 agaagaaaaa acaagttttt atattcaaaa agtgaaataa gctctgctaa ttatataagg
     8101 agaaaacatg tctgaaaaaa atgcttatgc ccagtctggt gttgatgtag aagcgggcta
     8161 cgaagttgtc gaacgtatca agaaacacgt tgctcgcaca gaacgtgctg gtgtcatggg
     8221 agctctcggt ggctttggtg gtatgtttga cctgtcacaa acaggtgtta aagaacctgt
     8281 cttgatttca gggactgacg gtgtcggaac taaactcatg cttgctatca agtacgacaa
     8341 gcacgacaca atcggtcaag actgtgttgc catgtgtgtc aacgatatta ttgcagcagg
     8401 tgctgagccc ctttacttcc ttgactatgt cgcgactggt aaaaacgaac ctgccaaatt
     8461 ggaacaggtt gtcgctggtg ttgctgaagg ttgtgttcaa gctagcgcag ctcttatcgg
     8521 tggtgaaacg gctgagatgc ctggtatgta tggcgaagat gattatgacc ttgcaggctt
     8581 tgctgttggt gtggctgaaa aatctcaaat catcgacggt tcaaaggtaa aagaagggga
     8641 tattcttctt ggacttgctt caagtggtat ccattcaaat ggttattcat tggtacgtcg
     8701 tgtctttgct gactacactg gtgatgaggt gcttccagag cttgaaggca aacaactcaa
     8761 ggatgtcctt cttgagccaa ctcgtatcta tgttaaagca gctctgccat tgatcaagga
     8821 agaactagtt aacggtatcg cccacatcac gggtggtggt tttatcgaga atgttcctcg
     8881 tatgtttgcg gatgatttgg ctgcggaaat cgatgaggat aaggtgcctg tacttccgat
     8941 tttcaaggcg cttgaaaaat atggtgacat caagcacgaa gaaatgtttg aaatcttcaa
     9001 catgggtgtc ggtcttatgc tggatgttaa ccctgaaaat gttgaccgtg tcaaagaact
     9061 tttggacgaa ccagtctatg aaatcggtcg tatcatcaag aaagcagacg atagtgtggt
     9121 gattaaataa gtcactaaat tttataagaa aggagtgttt agttagtttg cctaactgaa
     9181 cacgggatta aatttctagg aaaaaagata aaacgactta gacgcaagcg tcgttgttcg
     9241 ttttcctatt tttctacgaa atttttcggc aagccgaatc atccctttgt atcgaatatg
     9301 aaaatcgctg tttttgcttc tggtaatggt tccaactttc aggtcatagc agagcagttt
     9361 caagttagtt ttgtcttttc agatcatcgt gatgcctatg ttttagagcg tgctcagaac
     9421 ttagccattc caagcttcgc ttttgaactc aaagagtttg agaataaggc ggcttacgag
     9481 caagccgtcg ttgatttgtt ggacaaacac gagattgact tggtctgtct ggcgggctac
     9541 atgaagattg tcggagaaac cttgctctca gcctatgaag ggcgtatcat caatattcac
     9601 ccaacctatc tgcctgaatt tccaggtgct cacggtatca aggatgcttg ggaggcaggt
     9661 gttgaccagt ctggtgtgac catccattgg gtggactctg gtgtggatac cggtcaggtc
     9721 atccaacaag tgcacgtgcc acgcctagca gatgatagtc tagaaagctt tgaaacccgc
     9781 atccacgaaa ccgaatacca actctaccca gctgtcttag atagcttggg cataaagaga
     9841 aaatagtaaa atttgttata ataagctaat gaaactagtg aaaaatttag agatagtaga
     9901 gtctattttt ggtgattggg acgagaccat aatctggtcg tgtgtccaag gtattatggg
     9961 agaagttttt gtagatagtt tagaccaacc aaaatcttct ttggcaaaat tgggaaggaa
    10021 atcatctttt ggttttctag caggtcagcc tactttattt ctactagaag tttgctctgg
    10081 ggaggatatt atccttgttc ctcagcataa aggatggtca gatttgattg aatcaactta
    10141 tggtcagaat gctcattctt ttaagcgtta tgcgacgaaa aaagatactt tgtttgagcg
    10201 aagtaggtta gagaagtttg ttactcagtt gccaaatggt tttgaattac gtgctattga
    10261 tgagaaagtt tacaactctt gcttagaaaa ggaatggtca caggatttag tagcaaatta
    10321 cgctacttat cagtactata aaaaacaagg tataggttat gttgtatatt atcaggggaa
    10381 tatcattgca ggagcttcat cttattcaac ctataaaaat gggattgaga tagaagtaga
    10441 tacgcatcca gattttcgtc ggcgtggttt agcaacaata gttgcagctc agttaatact
    10501 aacttgttta gataagggaa tttatcctag ctgggatgct catacaagga cttcattgaa
    10561 tttatctgaa aaactcggtt atgaattttc tcatgaatac atagcctatg aaatagattg
    10621 aagtaagaaa aggagaactc atgactaaac gtgctttaat ctcagtttct gacaaatcag
    10681 gaattattga ctttgcaaaa gaattgaaaa acttgggttg ggatattatc tcaactggtg
    10741 ggactaaggt tgcccttgat gatgctggtg ttgagaccat tgccatcgac gatgtgactg
    10801 gattcccaga aatgatggac ggtcgtgtta agaccctcca cccaaacatt cacggtgggc
    10861 ttctggctcg tcgcgacgct gacagccacc ttcaggctgc taaggacaac aatattgagt
    10921 tgattgacct cgtggttgtc aacctctatc ccttcaagga gaccatcctt cgcccagacg
    10981 tgacctacga tttggcggtg gaaaatatcg acatcggcgg tccatcaatg cttcgctcag
    11041 ctgctaaaaa ccacgctagc gtaaccgttg tggttgattc agctgactat gccactgttt
    11101 tgggagaatt ggctgacgct agtcagacga catttaaaac tcgtcaacgc ttggcagcta
    11161 aggccttccg tcatacggca gcctacgacg ctttgattgc tgagtacttc acagctcaag
    11221 tgggagaggc taagcctgaa aaattgacca tcacttatga ccttaaacag gctatgcgtt
    11281 acggagaaaa tccacagcaa gacgctgatt tctaccaaaa agccttacca acagactact
    11341 caatcgcttc agctaaacag ctcaatggaa aagaattgtc cttcaataat atccgtgatg
    11401 ctgatgcagc aatccgtatt atccgcgatt tcaaagacag tccaacggtt gttgccctca
    11461 aacacatgaa cccatgtggt atcggacagg ctgatgatat tgagacagct tgggattacg
    11521 cttatgaagc tgatccagtt tcaatctttg gtggaattgt tgtccttaac cgtgaagttg
    11581 acgcagcgac agctgagaag atgcacccta tcttcttgga aatcatcatc gcaccatcat
    11641 actcagaaga agcgctagct attctcacaa ataaaaagaa aaacttgcgt atccttgagt
    11701 tgccgtttga tgcccaagct gccagcgaag tggaagctga gtacactggc gtagttggtg
    11761 gacttttggt gcaaaaccaa gacgttgtgg ctgaaaatcc atctgactgg caagtggtga
    11821 cagaccgcca gccaacagaa caagaggcga ctgcccttga gtttgcctgg aaggctatca
    11881 agtatgttaa gtctaacggg attattatta ctaacgatca catgacgctt ggactcggtg
    11941 caggtcaaac caaccgtgtc ggctcagtca agattgctat cgagcaggct aaggaccacc
    12001 ttgacggtgc cgttctagca tcagatgcct tcttcccatt tgcggacaac attgaagaaa
    12061 tcgctgccgc agggatcaaa gcaatcatcc aaccaggtgg ttcagttcgt gaccaagaat
    12121 ctattgacgc cgcaaacaaa catggcttga ccatgatctt cacaggcgtg agacatttta
    12181 gacattaaaa aatagtagtt taggttttct taagttaact caggtatact acgtatatcc
    12241 taaaaacttt tctttttatt aaacctccta agtactcatc aaggtgagta ctttttttga
    12301 tattttgatt taaattttcg aataagttaa ttgcaagtgt ttgagcactt cattatatta
    12361 attctatttg gaggtttttc ttgaataaat ggttagttaa ggcaagttcc ttagttgttt
    12421 taggtggtat ggttttatct gcgggttccc gagttttagc ggatacttat gtccgtccaa
    12481 ttgataatgg tagaattaca acaggtttca atggttatcc tggacattgt ggggtggatt
    12541 atgctgttcc gactggaacg attattaggg cagtggcaga tggtactgtg aaatttgcag
    12601 gagctggagc caacttttct tggatgacag acttagcagg aaattgtgtc atgattcaac
    12661 atgcggatgg aatgcatagt ggttacgctc atatgtcacg tgtggtggct aggactgggg
    12721 aaaaagtcaa acaaggagat atcatcggtt acgtaggagc aactggtatg gcgacgggac
    12781 ctcaccttca ttttgaattt ttaccagcta accctaattt tcaaaatggt ttccatggac
    12841 gtatcaatcc aacgtcacta attgctaatg ttgcgacctt tagtggaaaa acgcaagcat
    12901 cagctccaag cattaagcca ttacaatcag ctcctgtaca gaatcaatct agtaaattaa
    12961 aagtgtatcg agtagatgaa ttacaaaagg ttaatggtgt ttggttagtc aaaaataaca
    13021 ccctaacgcc gactgggttt gattggaacg ataatggtat accagcatca gaaattgatg
    13081 aggttgatgc taatggtaat ttgacagctg accaggttct tcaaaaaggt ggttacttta
    13141 tctttaatcc taaaactctt aagactgtag aaaaacccat ccaaggaaca gctggtttaa
    13201 cttgggctaa gacacgcttt gctaatggta gttcagtttg gcttcgcgtt gacaacagtc
    13261 aagaactgct ttacaaatag tttgaggtat tgattcattg ttttaaatga cagttgtgtt
    13321 actaactaag tacaatttct ttaaaccgtc tgaaaataat tttatagtcc agtaaagtgt
    13381 gatattatag tctcggacta ataaaaagga aataggaatt gaagcaatga aaatgaataa
    13441 aaaggtacta ttgacatcga caatggcagc ttcgctatta tcagtcgcaa gtgttcaagc
    13501 acaagaaaca gatacgacgt ggacagcacg tactgtttca gaggtaaagg ctgatttggt
    13561 aaagcaagac aataaatcat catatactgt gaaatatggt gatacactaa gcgttatttc
    13621 agaagcaatg tcaattgata tgaatgtctt agcaaaaata aataacattg cagatatcaa
    13681 tcttatttat cctgagacaa cactgacagt aacttacgat cagaagagtc atactgccac
    13741 ttcaatgaaa atagaaacac cagcaacaaa tgctgctggt caaacaacag ctactgtgga
    13801 tttgaaaacc aatcaagttt ctgttgcaga ccaaaaagtt tctctcaata caatttcgga
    13861 aggtatgaca ccagaagcag caacaacgat tgtttcgcca atgaagacat attcttctgc
    13921 gccagctttg aaatcaaaag aagtattagc acaagagcaa gctgttagtc aagcagcagc
    13981 taatgaacag gtatcaccag ctcctgtgaa gtcgattact tcagaagttc cagcagctaa
    14041 agaggaagtt aaaccaactc agacgtcagt cagtcagtca acaacagtat caccagcttc
    14101 tgttgccgct gaaacaccag ctccagtagc taaagtagca ccggtaagaa ctgtagcagc
    14161 ccctagagtg gcaagtgtta aagtagtcac tcctaaagta gaaactggtg catcaccaga
    14221 gcatgtatca gctccagcag ttcctgtgac tacgacttca ccagctacag acagtaagtt
    14281 acaagcgact gaagttaaga gcgttccggt agcacaaaaa gctccaacag caacaccggt
    14341 agcacaacca gcttcaacaa caaatgcagt agctgcacat cctgaaaatg cagggctcca
    14401 acctcatgtt gcagcttata aagaaaaagt agcgtcaact tatggagtta atgaattcag
    14461 tacataccgt gcgggagatc caggtgatca tggtaaaggt ttagcagttg actttattgt
    14521 aggtactaat caagcacttg gtaataaagt tgcacagtac tctacacaaa atatggcagc
    14581 aaataacatt tcatatgtta tctggcaaca aaagttttac tcaaatacaa acagtattta
    14641 tggacctgct aatacttgga atgcaatgcc agatcgtggt ggcgttactg ccaaccacta
    14701 tgaccacgtt cacgtatcat ttaacaaata atataaaaaa ggaagctatt tggcttcttt
    14761 tttatatgcc ttgaatagac tttcaaggtt cttatataat ttttattaaa ttgaggagat
    14821 taagctataa gtctgaaact actttcacgt taaccgtgac taaatcaaaa cgttaaaact
    14881 aaaatctaag tccgtaaaga ttattgaaaa cgctttaaaa atagatataa taaggtttgt
    14941 agatatctaa aattaaaaaa gataaggaag tgagaatatg ccacatctaa gtaaagaagc
    15001 ttttaaaaag caaataaaaa atggcattat tgtgtcatgt caagctttgc ccggggagcc
    15061 tctttatact gaaagtggag gtgttatgcc tcttttagct ttggcagctc aagaagcagg
    15121 agcggttggt ataagagcca atagtgtccg cgacattaag gaaattcaag aggttactaa
    15181 tttacctatc atcggcatta ttaaacgtga atatcctcca caagaaccat ttatcactgc
    15241 tacgatgaca gaggtggatc aattagctag tttagatatt gcagtaatag ccttagattg
    15301 tacacttaga gagcgtcatg atggtttgag tgtagttgag tttattcaaa agataaaaag
    15361 gaaatatcct gaacagttgt taatggctga tataagtact tttgaagaag gcaaaaatgc
    15421 ttttgaagca ggagttgatt ttgtgggtac aactctatct ggatacacag attacagccg
    15481 ccaagaagaa ggaccggata tagaactcct taataagctc tgtcaagccg gtatagatgt
    15541 gattgcggaa ggtaaaattc atactcctaa gcaggctaat gaaattaatc atataggtgt
    15601 tgcaggaatt gtagttggtg gtgctatcac tagaccaaaa gaaatagcgg agcgtttcat
    15661 ctcaggactt agttaaaagt gttactcaaa aatcaaaatc aaaataaaaa aggggaatag
    15721 ttatgagtat caaaaaaagt gtgattggtt tttgcctcgg agctgcagca ttatcaatgt
    15781 ttgcttgtgt agacagtagt caatctgtta tggctgccga gaaggataaa gtcgaaatta
    15841 cgtggtgggc ttttccaacc tttactcaag aaaaggctaa ggatggagta ggtacttatg
    15901 agaaaaaagt catcaaggct tttgaaaaga aaaatcctaa tataaaagta aaactagaga
    15961 caattgattt cacatctgga cctgaaaaaa tcactacagc gattgaagca gggacagcac
    16021 ctgatgtgct ttttgatgca ccagggcgaa ttattcaata tggtaaaaat ggtaaattag
    16081 cagatttgaa tgatttattt acagaccaat ttattaagga tgtcaataat aagaatatca
    16141 ttcaagcttc taagtctggc gataaagcct acatgtatcc aataagttct gccccatttt
    16201 atatggcgtt caataaaaaa atgcttaaag atgcaggagt tttgaaactc gtaaaagaag
    16261 gttggactac tagtgatttt gaaaaagtac taaaagcact aaaaaataaa ggctatacac
    16321 caggttcatt ctttgcaaac gggcaaggag gagatcaagg accacgtgca ttttttgcta
    16381 atctttatag tgctccaata acagataaag aagtaacaaa atataccact gacactaaaa
    16441 attctgtaaa atcaatgaaa aaaatagttg aatggattaa gaaaggctac ttgatgaatg
    16501 ggtctcagta tgatggctca gctgacattc aaaactttgc caatggacaa actgctttca
    16561 ctatcctatg ggctccagct caacccaaaa ctcaagcaaa attattagag tcaagtaaag
    16621 tggattacct tgaagtgcca ttcccatcag aagatggaaa accagattta gaataccttg
    16681 ttaatggttt tgcggtcttt aataataaag atgaaaacaa agtaaaagcc tctaagaaat
    16741 ttatcacttt tattgctgac gataaaaaat ggggaccaaa agatgttata cgtacaggtg
    16801 ctttcccagt tagaacatca tttggagatc tttataaagg tgataaacgt atgatgaaga
    16861 tttcaaaatg gactcaatat tattcaccat attacaacac tatcgatgga ttttctgaaa
    16921 tgagaacctt atggttccca atggttcaat ctgtatccaa tggtgacgaa aaaccagcag
    16981 atgctttgaa agactttact caaaaagcaa atgataccat taaaaaagca gctaaataat
    17041 taaggagact ccttttctat ggtcaatcac atattgggat agaagaggag tttttagtca
    17101 agttaagatg taaaggagag taccgtatgc gtactaataa gttaaagatg agggaaacga
    17161 tgatagctta tgcttttcta gcacctattc tcttattttt cttaattttt gtttttgcac
    17221 cgatggttat gggctttgtc actagttttt ttaattattc tatgacacag ttcactttta
    17281 ttgggttagc taattacaat cgtatgtttc atgattctat tttcatgaaa tccttgataa
    17341 atacagtaat cattgtgatt ggctctgtgc cagtagtggt tttcttctca ctttttgttg
    17401 ctgccaatac ttatgaaaaa aatgtttttt cacgctcttt ctaccgttgt gtcttcttct
    17461 tgccagtagt aactggtagt gttgcagtta cagttgtttg gaaatggatt tatgacccta
    17521 tgtccggaat tttaaattac atcttgaaat caggtcatgt cattgaacag aatattagct
    17581 ggcttgggga taagcactgg gcattgcttg ctattattat tattttgcta acgacctcag
    17641 ttggtcaacc tattattcta tatattgcgg cgatgggaaa tattgataat tctctttgcg
    17701 aagctgcgcg tgttgatgga gccaatgaaa tgcaagtttt ttggcaaatt aaatggccga
    17761 gtctcttacc aactacactc tatattgcag ttataacaac aattaattca tttcaatgtt
    17821 ttgccttaat ccagctccta acatctggtg gaccaaatta ttcaacaagc actttgatgt
    17881 attatcttta tgaaaaagcc tttaaactat cagaatatgg ctatgctaat actatgggag
    17941 tattcctagc agtaatgatt gctttaataa gttttgctca atttaagatc ttaggaaatg
    18001 atgtggaata ttaggaggaa aacatgaaaa agaaaacatt cagtgcttat aactttttaa
    18061 cggctcttat cctttgtctt ttgacagtgc tttttatctt tccattttat tggattatga
    18121 caggagcttt taaatctcaa cctgatacta ttatcatccc cccacaatgg tggcccaaag
    18181 caccgacctt agaaaatttt aaagctttaa ctgtacaaaa cccagctttg agatggcttt
    18241 ggaatagtgt ctttatctca attatgacaa tgttcctagt ttgctgtaca tcgtcaatgg
    18301 caggctatgt tttagctaag aaacgatttt acggtcaaaa aatattgttc tcccttttta
    18361 ttgctgctat ggctttacct aaacaagttg ttttagtgcc attagttaga attatcaatt
    18421 ttatggggat acatgatacc ttatgggcgg ttattttacc tcttgttggt tggccttttg
    18481 gggtcttctt gatgaaacag ttttctgaaa atattccaac agaattactg gaatcagcta
    18541 aaattgatgg ttgtggagag attcgaacat ttattaatgt agcatttcca attgtcaaac
    18601 ctggatttgc tgccctagct attttcacat ttatcaatac ttggaatgac tattttatgc
    18661 aattagttat gctaacttct agaaataatc tgaccatttc tcttggggta gctactatgc
    18721 aggctgaaat ggcaacaaac tatggcttaa ttatggcagg tgcagcctta gctgctgttc
    18781 ctattgtgac agtattctta gttttccaaa aatcctttac tcaggggatt acaatgggag
    18841 ctgttaaagg ataggtagga tttattatga tttacgatca tttattgaat ttaacgcatt
    18901 ataaagatat taatcctaat ttagatttag cgattgatta tctactaagt catgatttaa
    18961 ggaatttaga tataggcacc tatcatatta gcccagaagt gatactcatg gtccaatcaa
    19021 atcaacttag tgagtcattt gatcatattt ttgaatacca taaaaaatac cttgatatac
    19081 attatgtcat tgaaggacat gaagttatca aattgggaaa aggtgataag gtagaggtgg
    19141 aggaatactt aggcgatata ggatttatca agtgctcaga agagacttct tttgatttga
    19201 gagataatta tatagccttc tttttcccag aagaagctca tcagccaaat ggtatgggaa
    19261 gtctaggtaa ttatgtcaaa aaaggggtat tgaaagtgtt aatgacgtaa gaaatacagg
    19321 gagtatattg tgaaaaaagc aaatcaatta atagcagcga tttttgatgt taacaacccc
    19381 tttatgcaag gttgcaatgt tgtttttgat ctggcattac taaaccttct ctttatgata
    19441 acttgcctac cattggtaac tattggtgct gctaaaatta gtttgtatcg aacgttgtgg
    19501 caaaaattag aaggtgatca gacaaatctt ttaatattgt atataaaaca cctaaaaaaa
    19561 gagtggttcc aaggtatgct tttaggtttg gttgaactaa gtattttggt tgtgatcata
    19621 tttgatttaa caattcttca ttatcaaata ggtttcattg tatctttctt gaagataact
    19681 tgttacgcct ttttgttatt gactgtgatg acgagcatat acctatttcc tatggctgcc
    19741 cgctacgaaa tgtctctttt agataccgtg aaaaagagct ttataatggc atgtttaaac
    19801 ttgaaatgga ctggtgtatt gatgttttta ttaatcatga cttggtttat tatggtgcag
    19861 tctagtctct tatttatgct tacagtgtca gctatattta tttttgctta taccgccttt
    19921 gcttacttta aaattattat tttacaaaaa caatttgcct atttcagcaa acaacaagga
    19981 gattatcaat gaaagattta caaaaatatc aaggaattat tcctgcattt tatgcttgct
    20041 atgatgataa gggtgatatt tgtcccgaac gagtaaaagc cctgaccaat tattttattg
    20101 ataaaggtgt tcaaggactt tatgttaatg gttcatccgg agaatgtatt taccaaagtg
    20161 tagcagatcg taaacttgta ctggaaaatg ttatgtccgt tgctaagggt aaactaacag
    20221 taatagcgca tgtggcatgt aataatacta aggatagcgt agagttggct atgcatgctg
    20281 aagctatagg tgttgatgct attgcagcaa ttccaccaat ttattttcga ttaccagagt
    20341 atgccatagc agattattgg aatactatta gtcaagcagc accacaaact gattttatta
    20401 tttacaatat tcctcaattg gcaggggttg ctttgacatc ggatttatat cgaaaaatgt
    20461 tacaaaatcc gcaagtaatt ggtgttaaaa attcttccat gccagttcaa gacattcaga
    20521 attttgtggc tattggagga gaaaatcata ttgtttttaa tggtccagat gaacaatttt
    20581 tgggtggtcg tctcatggga gccgctgcag gtattggtgg tacctatggc gtaatgccag
    20641 aattatactt gactttaaat cagttaattg ttgataaaga tttagaaaaa gcgcgtgaac
    20701 ttcaatttac aattaacgat attatcacaa agctttgttc gggtcatggt aatatgtatg
    20761 ctgtgatcaa agctgtattg gaaatcaatg agcaactaac tataggttcc gttcggttac
    20821 ctttagcatc tgtaacagaa gaagataaac ctattattaa agaggcagca gaaatgatta
    20881 gacatgctaa aaaacagttt tgttaaagtg aggtagataa ttatgaccag aacagtagca
    20941 attgatattg gcggtacaat gattaagcat ggcattgtgg ataatttagg ttgtattgtt
    21001 gaggcaagtg agcttgccac agaagcctac aaaggtggtc caggcatttt acaaaaagtt
    21061 tgtcaaatta tcgataacta tctagcagaa gggagcattg atggaattgc tatctcatct
    21121 gctggcatgg tagatccaga tgaaggatgt atattttact ctggtcctca aattccaaac
    21181 tatgctggga cacaatttaa gaaagtttta gaagacactt atcaagtacg cacagaaatt
    21241 gaaaatgatg ttaattgtgc aggattagct gaagctgtct caggtagtgc caaagatagt
    21301 tcaatagcgc tttgtcttac tattggaaca ggtattggtg gttgtctgat tattgataaa
    21361 acagtttttc atggctttag taattctgct tgtgaagttg gctatatgca cctttcagat
    21421 ggtgattttc aagatttagc ctctacaact gcactgattg ctgatgttgc caaagcgcat
    21481 ggagatgaaa ttagccgatg ggatggacgt cgtatttttc aggaggctaa aaaaggcaat
    21541 gaaaaatgta ttgcaagtat agaccgtatg ataaactacc ttggccaagg aatcgctaat
    21601 atggtttatg tggtaaatcc tgaaaaagtt gttttaggtg gtggaatcat ggctcaaaaa
    21661 gattatttac aagataaact tagtgaatct ttgaaacgaa atcttgtgac aagtcttgct
    21721 gaaaaaacag caatagtctt tgcacaacac gaaaaccaag ctggtatgct aggagcttac
    21781 taccatttta aaaacagggg gtgacgggta tatgattgaa acaatgtcgc tagatgacat
    21841 gagagaatac ctaggtcaag accaaattcc cgaggatttt gatgacttct ggaaaaaaca
    21901 aacaatgaaa tatcaaggga atattgagta tcgtctagac aaaaaagatt tcaatattac
    21961 ttttgcacaa gcttatgatc ttcattttaa aggcagcaat aactctattg tctatgccaa
    22021 atgtttattc cctaaaacaa ataaacccta tcctgtagtt ttttattttc atggttacca
    22081 aaaccaaagt cctgactgga gtgaccaact taattatgta gcagcaggtt acggcgttgt
    22141 ttcaatggat gttcgaggtc aagctggaca gtctcaagac aaggggcatt ttgatggcat
    22201 tacagttaag ggccagattg tgcgaggaat gatctcagga ccaaatcatc ttttttataa
    22261 agacatttat cttgatgttt tccaattgat tgatatcatt gctacattag agagtgttga
    22321 tagtaatcaa ctttatagtt atggctggtc tcaaggtgga gcacttgctt tgatagctgc
    22381 agctcttaat cccaaaatcg tcaagaccgt tgctgtttac ccatttttgt cagactttag
    22441 acgagtatta gatttagggg gtgtcagtga gccctatgat gaactcttcc gctattttaa
    22501 atatagtgat ccttttcata aaacagagaa taatgtgtta aaaactttgg cttatattga
    22561 tgttaaaaat tttgcacata ggatttcttg ccccgttgtt ttactaacag ccttaaaaga
    22621 tgatatttgt cctccatcaa ctcaatttgc catttttaat cgtctgacat caactaaaaa
    22681 gcatctttta ttaccagatt atggacatga tccaatgaca gtgcaagtaa aagaccatat
    22741 ttttgatcaa ctgacaggaa gtcaatttac aaagcaaaaa atcgagtaga ttatttctac
    22801 tcgattttca tttagcgttg aataatggtt tctctaaaaa ttcgctcttt ttctatttta
    22861 ttaatatcta aaaattgtgc atagataata tctaccatga ttaacattgg gatctgaggc
    22921 gaaattctat tgccgtagtc taaatgccta gcagaagcca cctgaataat cttgtcaaaa
    22981 gcatagtcaa ttgtgtgagg ttgttttgta aatagaacgg ttttagctcc gtgacaactt
    23041 gcgattttta aggcaccaat aacagagttg gtgttcccag aaagtgagaa ggcaatgacg
    23101 aggcatctat cattaacaat actattagtc catgagaaac tatctgtatc atccagagcc
    23161 tcacaaatca ctcccagcct cataaggcgt attttaaatt ctttagctac tagcgatgag
    23221 ctccctttgc cataaaaata aacacgatca gcttgctcaa tcatatgtga tacttctaga
    23281 agctgttctt catcaataat atcagcagat ttagaaatca tctcttgata attcataaat
    23341 actttcttag tgttgtcatt ttgaagaccg tataattgtt gactgatggt ctcatggctt
    23401 tttaaatatt caaaaacaaa ttcacgatag cctttaaacc cacatttttt agcaaaacgc
    23461 gttaaggctg cttgtgaaat gtgtagttgc ttagtgacaa tggtagaagc aagagcgtct
    23521 tttgaaatgg gctgccccat aaaataacaa gcaatttcac gttctaaatc agtcattaaa
    23581 tcaagagcag actcaatttg gtcataaata gtcataatac acggtcctct cctctttaag
    23641 aaagcgctta atatattcag tataacataa agttagtgag ggaccaaact aagttctaag
    23701 aaacagcaat gataagtttt aaatagcaat atgaaggatc aataacataa tatatgttaa
    23761 gtatatatta tgatataaat ttttcaaaaa agctgtaata aaaaaaataa aagcgaacaa
    23821 atgtgttata attttataaa tgatacgttt ttattgaaaa tttcttttta tattatttaa
    23881 taggagttat tctgatatga aattgcttgt tgttggttct ggtggtcgtg agcatgcgat
    23941 tgctaagaag ttgttagcgt ctaaggatgt ggatcaggtt tttgtggcac ctggtaatga
    24001 tggtatgacc ttggatggtc tagacttggt aaatatcgga atttccgaac attctagact
    24061 gattgacttt gttaaggaga atgagattgc ttggaccctt attggtcctg atgatgcgct
    24121 agcagctggt atcgttgatg gttttaatag tgctggactc agagcatttg gtccaaccaa
    24181 ggcagccgcg gagctagagt ggtcaaaaga ctttgccaag gaaatcatgg tcaaatacaa
    24241 tgttccaaca gcagcctatg gcacattttc agattttgaa aaagctaaag cctacatcga
    24301 agagcagggc gcaccaatcg tggtcaaggc tgacggattg gcgttaggca agggcgtggt
    24361 cgtggctgaa accgttgagc aggcggtaga ggcggcgcaa gagatgcttt tggacaacaa
    24421 gtttggcgac tcgggtgcgc gcgtggttat cgaggaattc ttggatggtg aagagttctc
    24481 ccttttcgcc ttcgctaatg gcgataagtt ctacatcatg ccgacagctc aggatcacaa
    24541 gcgtgcctat gatggtgaca aggggctaaa taccggtggt atgggtgcct atgcgccagt
    24601 tccccacctg cctcagagcg tggtggatac agcagttgag actatcgtta agcctgtcct
    24661 tgaaggcatg attgccgaag ggcgtcctta tctaggtgtc ctctatgctg ggcttatcct
    24721 gacggctgat ggccctaagg ttatcgagtt caactcacgt tttggtgacc ctgaaactca
    24781 gattatcctc cctcgcctga cttccgattt cgctcagaac atcgacgaca tcatgatggg
    24841 catcgagcct tacatcactt ggcagaagga cggcgtgact ctgggcgttg tcgttgcctc
    24901 agaaggctat ccgctcgatt acgagaaagg tgtgccactg cctgaaaaga ccgacggcga
    24961 catcatcacc tactatgcgg gagctaagtt tgcggaaaat agcaaagcac tgctctcaaa
    25021 cggaggacgt gtctacatgc ttgtcaccac agaagacagc gtcaaagcag ggcaggacaa
    25081 aatctatacc caactcgccc aacaagacac aacaggcctc ttctaccgaa acgacatcgg
    25141 aagcaaagct attaaggaat aagactactc attcaaatat tagcgtcagc aaagcgaccg
    25201 aaggtcgctc caaaaagaaa gaagaaagga actgaacacg ggactaattt tctagtgaaa
    25261 aagataaatc ttcctagatg ctaaagcatc ttcgtcagat ttctgcgtcg taggcgtctg
    25321 ctgctaaagc acctagtcgc cctgacgtgg gtacgtcttc gaaatcaaag atttcggact
    25381 taccgcatat ttttctacga aaattttcga caagtcgaaa cgtccctttg tatcttaata
    25441 ttatgcaacc aattatttct attatcatgg gttccaaatc cgactggaca accatgcaaa
    25501 aaacggctga agttctagat aactttggca ttgcttacga gaagaaagtc gtctctgccc
    25561 accgtacgcc agacctcatg ttcaagcatg ctgaggaggc gcgtggtcgt ggtatcaaga
    25621 ttatcatcgc tggagcaggt ggagcagccc acttgccggg tatggtagca gctaaaacaa
    25681 ccctgcctgt tatcggtgtg cctgtcaaat cacgtgccct ctcaggtttg gattcgctct
    25741 actctattgt gcagatgcct ggtggtgtgc ctgtggcgac catggctatc ggagaagcag
    25801 gagcgactaa cgctgccctg acagccctcc gtatcctctc tatcgaggat caaaatctgg
    25861 ctgatgcgct agctcatttc catgaggaac aaggaaaaat cgcagaggag tcaagcaatg
    25921 aactcattta agaccattgg gattattggt ggtggtcagc tggggcagat gatggcgatt
    25981 gcggctatct acatgggcca caaggtcatt acgctggatc cagctagcga ctgccctgcc
    26041 tcccgcgtta gcgaggtgat tgtggcacct tacgatgatg ttgaggcttt gggaacatta
    26101 gctgcgcgtt gcgatgtttt gacctatgag tttgagaatg tcgatgccga tggtctggat
    26161 gccgttgtgt cagctggtca gctaccgcag gggactgatc tgctccgcat ttctcaaaac
    26221 cgtatctttg aaaaagactt tctggcaaat aaggctggcg tgactgtcgc tccctataag
    26281 gtggtgacat ctagccttga cctagagggg cttgacttga ccaagaccta tgtcctcaag
    26341 acagcgacag gtggttatga cggtcatggg caaaaggtta tccgctcagc agaagacctg
    26401 ccagaggcgc agcaattagc caactcagct cagtgtgtct tggaagagtt tgtcaacttc
    26461 gaccttgaaa tatcagtcat cgtgtctgga aatggtcagg atgtgacggt ctttcccgtt
    26521 caggaaaata tccaccgcaa caatatcctg tcaaaaacca tcgtaccagc tcgcatctca
    26581 gaccaactag ctgacaaggc taaggaaatg gctgtgcaga ttgccaagaa actccagcta
    26641 tcaggaaccc tctgtgtgga aatgtttgcg accgcagatg acatcatcgt caatgaaatt
    26701 gccccacgtc cccacaactc agggcactac tctatcgaag cctgcgactt ttcacagttt
    26761 gacacccaca tcttgggcgt actgggcgca ccgcttccgc caatcaaact ccatgctcca
    26821 gccgttatgt tcaatgtcct aggacaacat gtccagcagg caattgacca tgttgcccaa
    26881 aaccctagcg cccacctcca catgtatggt aaactagaag caaaacataa ccgcaaaatg
    26941 ggacacgtga cggtgtttag cgatgtacct gatgaggtgg aagagtttga agaaaggatg
    27001 gatttctaag gaggtttatg gaattttagg ggggagaatg attttgaagg gagtgttgtc
    27061 agatgaatag aataataaac agggatattt tacctagaat ttcaaagatt tctaaaaata
    27121 ataaagagaa agatttacta tctatagctt atattacttg gctaatcttt ataatatttg
    27181 ctttgggagt agttacagtt aatgatttaa aaccgatgtt taatcaatta atagtaaacc
    27241 tcttaaatat ttattattat atggaagcct tcatattggg tatggacagc tatttacaat
    27301 ataaccttcc atacagtttt gatttttgga gtatttttgt agaggctata aatttatttg
    27361 taaaagtatt tttaattgct tttattcctt ctgttattag aaaagtattg aaaaaagaaa
    27421 gttttttcaa tgaagttgtt attttac
//
LOCUS       DATRMA010000023        24600 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.023, whole genome shotgun sequence.
ACCESSION   DATRMA010000023 DATRMA010000000
VERSION     DATRMA010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 24600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24600
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.023"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            complement(<1..1965)
                     /gene="recJ"
                     /locus_tag="VBC28_000902"
     CDS             complement(<1..1965)
                     /gene="recJ"
                     /locus_tag="VBC28_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEO6814903.1"
                     /translation="MISAKYSWVLNNQKPDAGFFEASKKEKISEAVASLIYSRGIKTS
                     AELHHFLQTNLENLHDPYLLNDMDKAVNRIRRAIENNETILVYGDYDADGMTSASIMK
                     EALDMMGAEVQVYLPNRFTDGYGPNQSVYKYFIEQQDVSLIITVDNGVAGHEAITYAQ
                     NQGVDVVVTDHHSMPADLPCAYAIIHPEHPDANYPFPYLAGCGVAFKVACALLETIPT
                     EMLDLVAIGTIADMVSLTDENRIMVKAGLEVMKDSERIGLQELISLSNIDLKTLNEET
                     IGFKIAPQLNALGRLDDPNPAIELLTGFDDEESQAIAQMIDQKNEERKEIVQTIFDQA
                     MQMLDQTKPVQVLAKENWHPGVLGIVAGRILERTGQPVIVLNIEDGIAKGSARSVEAL
                     DIFQAFDQHRELFIAFGGHSGAAGMTLEESKVGDLSQVLCDYISKKQLDMSQKKTLTI
                     DSELRFDELSLDTVRDFEKLAPFGMDNKKPVFLLKDFKVSQARVMGQNGAHLKLKLEQ
                     DGQALDLVAFNMGSQLQEFQQAQHLELAVTLSVNQWNGATTLQLMLEDARVDGIQLFD
                     IRSKASSLPHGVPILSQEEQSKEVILLTVPDHPQELKQMTQGKQFDAIYFKNEIPKNY
                     FISGYGTRDQFASLYKTIYQFPEFDVRYKLK"
     gene            complement(1962..2723)
                     /locus_tag="VBC28_000903"
     CDS             complement(1962..2723)
                     /locus_tag="VBC28_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEO6814904.1"
                     /translation="MARTILITGASGGLAQAIINQLPQDDHLIVTGRSREKLEKLYGK
                     RPNTLCLSLDITNDNAVTNMIEKIYGEFGQIDILINNAGFGSFKEFWDYSDEEVKDMF
                     AVNTFATMSIARQIGHKMSLVKSGHIVNIASMAGLIATSKASVYGASKFAVVGFSNAL
                     RLELAEKNVYVTSVNPGPIKTGFFAQADPSGDYLASIGRFALTPEKVSKKVVSILGKN
                     KRELNLPFILAFAHKYYSLFPKTADYFARKVFNYK"
     gene            complement(2725..3654)
                     /locus_tag="VBC28_000904"
     CDS             complement(2725..3654)
                     /locus_tag="VBC28_000904"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HEO6814905.1"
                     /translation="MEIQFLGTGAGQPAKARNVSSLVLKLLDEINEVWMFDCGEGTQR
                     QILETTIKPRKVKKIFITHMHGDHVFGLPGFLSSRAFQANEEQTDLDIYGPVGIKSFV
                     MTGLRTSGSRLPYRIHFHEFDESSLGKIMETDKFTVYAEKLDHTIFCMGYRVVQKDLE
                     GTLDAEALKLAGVPFGPLFGKVKNGENVTLEDGREIIAKDYISEPKKGKVITILGDTR
                     KTDASIRLALGADVLVHESTYGKGDERIAKSHGHSTNMQAADIAKQANAKRLLLNHVS
                     ARFMGRDCWQMEEDAKTIFSNTHLVRDLEEVGI"
     gene            complement(3696..4343)
                     /locus_tag="VBC28_000905"
     CDS             complement(3696..4343)
                     /locus_tag="VBC28_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HEO6814906.1"
                     /translation="MIDYIDLALTYGGFTSLDKVYLEKKLDGLSKQQRLDFITPPPSV
                     INAYFAEIYQKQGPEAATDYYFDLSKALGLFPKHLSFDEEKPFIRLNLSGKSFGFAYL
                     NDQEEASVFSEVKEVITPQLLLEIAQIFPQYKVYQDRSGIRMAKIDFDETESQNITPE
                     TSLLGNVLQLKKDIIKITSFNQEELLELVKTKSGKYYYSSQGRESVIYIDTKSTN"
     gene            complement(4336..5574)
                     /gene="hflX"
                     /locus_tag="VBC28_000906"
     CDS             complement(4336..5574)
                     /gene="hflX"
                     /locus_tag="VBC28_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEO6814907.1"
                     /translation="MIETKEEQERVILVGVELQDTENFEMSMEELASLAKTAGANVVN
                     HYYQKRDKYDSKSFIGSGKLEEIKAIVEADEIDTVVVNNRLTPRQNSNLEAELGVKVI
                     DRMQLILDIFAMRARSHEGKLQVHLAQLKYMLPRLVGQGIMLSRQAGGIGSRGPGESQ
                     LELNRRSIRHQISDIERQLKIVEKNRETVRERRVDSTTFKIGLIGYTNAGKSTIMNVL
                     TDDKQYEASELFATLDATTKQIYLQNQFQVTLTDTVGFIQDLPTELVAAFKSTLEESR
                     HVDLLLHVIDASDPNHEEHEKVVMEILKDLDMIDIPRLAIYNKMDVTEQLNATTFPNV
                     RIAAKKQGSRDLLRRLIVDEIRHIFDEFSIRVHQNQAYKLYDLNKIALLDTYTFEEEY
                     ENITGYISPKQKWKLEEFYD"
     gene            complement(5665..6555)
                     /gene="miaA"
                     /locus_tag="VBC28_000907"
     CDS             complement(5665..6555)
                     /gene="miaA"
                     /locus_tag="VBC28_000907"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEO6814908.1"
                     /translation="MRKIKLIAVVGPTAVGKTALGIELAKTFNGEIISGDSQQVYQKL
                     DIGTAKASKEEQEQAYHHLIDVREVNENYSVYDFVKEAKVAIDTIISKGKIPIIVGGT
                     GLYLQSLFEGYHLGGEVNQETLKAYREKLESLSDEDLFEKLTEQSIVIPQVNRRRAIR
                     ALELAKFGNDLQNSESPYDVLLIGLNDDRQVLYDRINRRVDLMIDNGLLDEAKWLYDN
                     YPSVQASRGIGYKELFPYFSKQIPLEEAVDKLKQNTRRFAKRQLTWFRNRMNVEFIMV
                     GEENYQQKIKRKVSDFLSSK"
     gene            6666..6842
                     /locus_tag="VBC28_000908"
     CDS             6666..6842
                     /locus_tag="VBC28_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HEO6814909.1"
                     /translation="MVKKFAFAKGIATGVVATAATLAGAAFAIKKTIIEPEEEKIAFI
                     EENRKKAARKRVSH"
     gene            complement(6884..7801)
                     /locus_tag="VBC28_000909"
     CDS             complement(6884..7801)
                     /locus_tag="VBC28_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HEO6814910.1"
                     /translation="MKNWEKPPLVMAGLVLGLLALGNLLEGYGTYFRYCLGLVALVFW
                     IFLIKGILKNKKESRKELSNPLIASVFTTFFMAGMILSTYILLFRSLGIWVAVLSKGV
                     WWLSFIALIIHMAIFSWKYLRHFSMANLFPSWSVLYVGIGVASLTAPISGQFTIGKIV
                     FWYGFIATLVLLPFLFIKAYKIGLPSAVKPNITTICAPMSLITARYVNSFVSPNRGLL
                     LLLIVMAQFLYFFILFQVPKLLIGDFTPGFSAFTFPLVISATSLKLSIQHLSLPVDIQ
                     GLVHFEIGTTTLIVMIVMVRYIFFLRRTI"
     gene            complement(7936..11694)
                     /locus_tag="VBC28_000910"
     CDS             complement(7936..11694)
                     /locus_tag="VBC28_000910"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pullulanase"
                     /protein_id="HEO6814911.1"
                     /translation="MKRKDLFGDKQTQYTIRKLSVGVASVATGVCIFLHSPQVFAEEV
                     SVSPATTAIAKSNINQVDNRQSTNLKDDINSNSETVVTPSDMPDTKQLVSDETDTQKG
                     VTEPDKATSLLEENKGPVSDKNTLDLKVAPSTLQNTPDKTSQAIGAPSPTLKVANQAP
                     QIENGYFRLHLKELPQGHPVESTGLWIWGDVDQPSSNWPNGAIPMTNAKKDDYGYYVD
                     FKLSEKQRKQISFLINNKAGTNLSGDHHIPLLRPEMNQVWIDEKYGIHTYQPLKEGYV
                     RINYLSSSGNYDHLSAWLFKDVATPSTTWPDGSNFVNQGLYGRYIDVPLKTNAKEIGF
                     LILDESKTGDAVKVQPNDYVFRDLANHNQIFVKDKDPKVYNNPYYIDQVQLKDAQQTD
                     LTSIQASFTTLDGVDKTEILKELKVTDKNQNAIQISDITLDTSKSLLIIKGDFNPKQG
                     HFNISYNGNNVTTRQSWEFKDQLYAYSGNLGAVLNQDGSKVEASLWSPSADSVTMIIY
                     DKDNQNRVVATTPLVKNNKGVWQTILDTKLGIKNYTGYYYLYEIKRGKDKVKILDPYA
                     KSLAEWDSNTVNDDIKTAKAAFVNPSQLGPQNLSFAKIANFKGRQDAVIYEAHVRDFT
                     SDQSLDGKLKNQFVTFAAFSEKLDYLQKLGVTHIQLLPVLSYFYVNEMDKSRSTAYTS
                     SDNNYNWGYDPQSYFALSGMYSEKPKDPSARIAELKQLIHDIHKRGMGVILDVVYNHT
                     AKTYLFEDIEPNYYHFMNEDGSPRESFGGGRLGTTHAMSRRVLVDSIKYLTSEFKVDG
                     FRFDMMGDHDAAAIELAYKEAKAINPNMIMIGEGWRTFQGDQGKPVKPADQDWMKSTD
                     TVGVFSDDIRNSLKSGFPNEGTPAFITGGPQSLQGIFKNIKAQPGNFEADSPGDVVQY
                     IAAHDNLTLHDVIAKSINKDPKVAEEEIHRRLRLGNVMILTSQGTAFIHSGQEYGRTK
                     RLLNPDYMTKVSDDKLPNKATLIEAVKEYPYFIHDSYDSSDAINHFDWAAATDNNKHP
                     ISTKTQAYTAGLITLRRSTDAFRKLSKAEIDREVSLITEVGQGDIKEKDLVIAYQTID
                     SKGDIYAVFVNADSKARNVLLGEKYKHLLKGQVIVDADQAGIKPISTPRGVHFEKDSL
                     LIDPLTAIVIKVGKVAPSPKEELQADYPKTQSFKGSKTVEKVNRIANKTSITPVVSNK
                     TDSYLTNEANLPKTGDKSSKILSVVGISILASLLALLGLSLKRNRHLK"
     gene            complement(11863..12597)
                     /locus_tag="VBC28_000911"
     CDS             complement(11863..12597)
                     /locus_tag="VBC28_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6814912.1"
                     /translation="MRDNHLHTHFSYDCQTAFEDYINGFTGEFITTEHFDLSNPYTGQ
                     DDVPDYSAYCQKIDYLNQKYGNRFKKGIEIGYFKDRESDILDYLKNKEFDLKLLSIHH
                     NGRYDYLQEEALKVPTKELLADYFNRMEFAIGRVEAHVLDHFDYGFRKLNLDVEDLKP
                     FETQLKRIFRKMLSKGLAFELNTKSLYLYGNEKLYRYALEILKQLGCKQYSIGSDGHI
                     PEHFCYEFDRLQCLLKDYQIDENHLI"
     gene            complement(12612..13196)
                     /locus_tag="VBC28_000912"
     CDS             complement(12612..13196)
                     /locus_tag="VBC28_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000130595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEO6814913.1"
                     /translation="MTDLEKIIKAIKSDSQNQNYTENGIDPLFAAPKTARINIVGQAP
                     GLKTQEARLYWKDKSGDRLRQWLGVDEETFYHSGKFAVLPLDFYYPGKGKSGDLPPRK
                     GFAEKWHPLILKEMPNVQLTLLVGQYAQKYYLGSSAHKNLTETVKSYKDYLPDYLPLV
                     HPSPRNQIWLKKNPWFEKDLIVDLQKIVADILKD"
     gene            complement(13293..14699)
                     /gene="pepV"
                     /locus_tag="VBC28_000913"
     CDS             complement(13293..14699)
                     /gene="pepV"
                     /locus_tag="VBC28_000913"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HEO6814914.1"
                     /translation="MTIDFRAEVDKRKDALMDDLINLLRINSERDDSQADAEHPFGPG
                     PVKALEFFLEMAERDGYETKNVDNYAGHFTFGQGEEELGIFGHLDVVPAGSGWDTDPY
                     EPVIKDNRLYARGSSDDKGPTMACYYALKIIKELGLPTSKKVRFVVGTDEESGWGDMD
                     YYFEHVGLPKPDFGFSPDAEFPIINGEKGNITEYLHFSGENKGAVRLHSFSGGLRENM
                     VPESATARFTSHLDQTTLGASLADFAGKHNLKTELSVEDGQYIATVYGKSAHGSIPQE
                     GVNGATYLALYLSQFDFEGPARAFLDVTANIIHEDFSGEKLGVAYEDDCMGSLSMNAG
                     VFQFDETNDDNTIALNFRYPQGTDAKTIQTKLEKLNGVEKVTLSDHEHTPHYVPMDDE
                     LVSTLLAVYEKQTGLKGHEQVIGGGTFGRLLERGVAYGAMFPGDENTMHQANEYMPLE
                     NIFRSAAIYAEAIYELIK"
     gene            complement(14746..15348)
                     /locus_tag="VBC28_000914"
     CDS             complement(14746..15348)
                     /locus_tag="VBC28_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEO6814915.1"
                     /translation="MKFLELNKKRHAVKHFNDKPVDFKDVRTAIEIATLAPSANNIQP
                     WKFVVVQEKKSALAEGLPESNCNQINQAQYVIALFTDTDLGQRSRKIARIGRRSLPDD
                     LIGYYMETLPPRYALYSEKQTGEYLSLNAGIVAMNLVLALTDQGISSNMILGFDKAIT
                     NDVLEIDKRFRPEILITVGYSDEKVEPSYRLPVDHIIEKR"
     gene            15518..17299
                     /locus_tag="VBC28_000915"
     CDS             15518..17299
                     /locus_tag="VBC28_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000942633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEO6814916.1"
                     /translation="MLRALKEDFFLTLTSLKNQFWVYIGTGILIQTLVAYLVKGILSF
                     IFRRILILSNTPAVTKDNWTLLFNHPLALLLFILYIVILIGFIYTEFTIYTIAILKTE
                     FSLSIKKWLTIFPKKIKSLLGPQLIWVSIYLLLTIPLANLGLRSSILEHLKIPDFISG
                     ELTKTVFGKIGYSTLLLLICYLNLRLIYFLPLTILTDYNAKEALLESWKLSRGKRQWR
                     LLSKIILTSLFISVIGTVALAVVAGTLSFIDHSGNNFPLQATFYNLLKSILFAVTLLI
                     KCLIISHLLADIKQNKSIVQQWRAWKGVHHKQKFKHIACVILALAIGTTALKNAIALA
                     LLQDNISIKKELIAHRGDTSHGVENSIEALKVAHKAKADYSEMDVIMTKDHKLVVIHD
                     DNLKRLSGMNKDVSKLTLDQVTKIPIHQGHFASYIPSFAEFMKTAQSLDQKIMIELKP
                     YNQNLDIYADEFIKEFKELRLSTKHKVMSLNLTLIEKVEKKLPQLDTGYLIPLHWGTL
                     QNHNVDFYGIEEFSYNDWIAYLAQEYNKQLYVWTINRDNLMIRYLQSPVNGIITDKLN
                     LFKVINKDIKNSPNYYQRALQLIDSEG"
     gene            complement(17341..19122)
                     /gene="uvrC"
                     /locus_tag="VBC28_000916"
     CDS             complement(17341..19122)
                     /gene="uvrC"
                     /locus_tag="VBC28_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEO6814917.1"
                     /translation="MNELIKHKLELLPDSPGCYLHKDKNGTIIYVGKAKNLKNRVKSY
                     FHGSHNTKTELLVSEIEDFEYIVTTSNTEALLLEINLIQENMPKYNIRLKDDKSYPYI
                     KITNERYPRLMITRQVKKSDGTYFGPYPDSGAATEIKRLLDRLFPFKKCTNPANKVCF
                     YYHLGQCNAHTVCQTNKAYWDSLREDVKQFLNGKDNKIVNGLTEKMKSAAMTMEFERA
                     AEYRDLIEAISLLRTKQRVIHQDMKDRDVFGYFVNKGWMCVQVFFVRNGKLIQRDVNM
                     FPYYNEPEEDFLTYIGQFYQDTKHFLPKEVFIPQDIDAKSVETIVGCKIVKPQRGEKK
                     QLVNLAIKNARVSLQQKFDLLEKDIRKTHGAIENLGNLLNIPKPVRIEAFDNSNIQGT
                     SPVAAMVVFVNGKPSKKDYRKFKIKTVIGPDDYASMREVIHRRYSRVLKDGLTPPDLI
                     VIDGGQGQVNIARDVIENQLGLAIPIAGLQKNDKHQTHELLFGDPLEVVELPRNSEEF
                     FLLHRIQDEVHRFAITFHRQLRSKNSFSSKLDGITGLGPKRKQLLMKHFKSLPNIQKA
                     DIEDIIMCGIPRTVAESLRDSLNDPPK"
     gene            19281..20048
                     /locus_tag="VBC28_000917"
     CDS             19281..20048
                     /locus_tag="VBC28_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HEO6814918.1"
                     /translation="MIDVENILEKMKPNQKINYDWVMQQMVKQWQASDIRPKILMHVC
                     CAPCSTYTLEYLSQWADVTIYFANSNIHPKDEYYRREYVTQKFVHDFNKNTGYSVQFL
                     SAPYEPNEFFKIVHGLEEEPEGGDRCKVCYDFRLDKTAEKAVELGFDYFGSALTISPH
                     KNSQTINTIGIDVQKIYDTQYLPSDLKKNKGYQRSVEMCKDYDIYRQCYCGCIFGAKD
                     QGINLLQIKKDAKAFVSDKDGKEEFPNIRFTFNGKSM"
     gene            complement(20108..21448)
                     /locus_tag="VBC28_000918"
     CDS             complement(20108..21448)
                     /locus_tag="VBC28_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEO6814919.1"
                     /translation="MYQTQTNKEKFVLFLKLFIPVLIYQFANFSATFIDSVMTGQYSQ
                     LHLAGVSTASNLWTPFFALLVGMISALVPVVGQHLGRGNKEQIRTEFHQFLYLGLILS
                     LILFLIMQFIAQPVLGSLGLEDEVLAVGRGYLNYMLIGIMPLVLFSICRSFFDALGLT
                     RLSMYLMLLILPFNSFFNYMLIYGKFGMPRLGGAGAGLGTSLTYWAIFIVIIIVMSLH
                     PQIKTYHIWTLERIKAPLIIEDIRLGLPIGLQIFAEVAIFAVVGLFMAKFSSIIIAAH
                     QAAMNFSSLMYAFPLSISTALAITISFEVGAERFQDANTYSRIGRLTAVGITSGTLLF
                     LFLFRENVAAMYNSDPHFVAITAQFLTYSLFFQFADAYAAPVQGILRGYKDTTKPFMI
                     GAGSYWLCALPLAVILEKNSQLGPFAYWIGLITGIFVCGLFLNQRLQKIKKLYY"
     gene            complement(21548..21964)
                     /locus_tag="VBC28_000919"
     CDS             complement(21548..21964)
                     /locus_tag="VBC28_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEO6814920.1"
                     /translation="MVMKAIEIYMNINGRGAEAITFYEEAFNTKAEHVTTFAQGWPDC
                     PPELEHLIMNAQFNIDGIRMQLSDNNPIFDFQIGQNMTAALIVESVEEAQELFNKLSR
                     DAMNIKMPLQAVPWSPAYGNVTDKYGMTWQINTELS"
     gene            complement(21968..22465)
                     /locus_tag="VBC28_000920"
     CDS             complement(21968..22465)
                     /locus_tag="VBC28_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEO6814921.1"
                     /translation="MIIDFKTVSTKGLETSPVAEALAGLRANEARYFWNKFKVAYETF
                     PVSDYPELMADINHLLKNERNLVFAAKPLEISQLFLENRILWTHVFYEDGLAINILYE
                     LDNPKKRAVGLKLSDGMDIPIELADKFKFARQKSKLAGIIRGSYFTLKGENETLMQRW
                     LDNMK"
     gene            22687..23283
                     /locus_tag="VBC28_000921"
     CDS             22687..23283
                     /locus_tag="VBC28_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ltp family lipoprotein"
                     /protein_id="HEO6814922.1"
                     /translation="MTKKIFLFICATLLVGIALYTPTTSIFNHNNVYAATKKAKIKFN
                     KTQKKIVKKAREYAKSGHMSKDSIIEKLKKDSKKYRQEDINFVINNLKVDYKKNALIS
                     AKIYSKTMNLSKQSIFEQLYSESPDKATHSDKFTKEESQYAIDHLKVDFKENALETAK
                     SYQSSSSLSKEEIYKQLTSTLGDKFTNDEAQYAVDHLK"
     gene            23472..24577
                     /locus_tag="VBC28_000922"
     CDS             join(23472..23748,23748..24577)
                     /locus_tag="VBC28_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612000.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISSag2 family transposase"
                     /protein_id="HEO6814923.1"
                     /translation="MSRKVRRHFTDDFKQQIVDLYNVGRKRSSLIKVYELTPSTFDKW
                     VRQAKTTGSFKSVDNLTDEQRELIELRKHNKELEMQLDILKQAAVIMAPKREIITANK
                     DKYSISKMCRWLNMPHSSYYYQAVESVSETEFEETIKRIFLDSESRYGSRKIKICLNN
                     EGITLSRRRIRRIMKRLNLVSVYQKATFKPHSRGKNEAPIPNHLDRQFKQERPLQALV
                     TDLTYVRVGNRWAYVCLIIDLYNREIIGLSLGWHKTAELVKQAIQSIPYALTKVKMFH
                     SDRGKEFDNQLIDEILEAFGITRSLSQTGCPYDNAVAESTYRAFKIEFVYQETFQLLE
                     ELALKTKDYVHWWNYHRIHGSLNYQTPMTKRLIA"
BASE COUNT         7950 a         4769 c         3771 g         8110 t
ORIGIN      
        1 ttttaactta taacgaacgt caaattctgg gaattgatat attgttttat ataaactagc
       61 aaattgatca cgtgtaccat aaccagatat aaaataattt tttggtatct catttttaaa
      121 atagatagca tcaaactgtt taccttgagt catttgtttt aattcttgtg gatgatcggg
      181 aacagtcaaa agaataactt ctttgctctg ctcttcttga gacaggattg gcactccatg
      241 aggtaagcta cttgctttgc ttctaatatc aaataattgt ataccatcta cacgcgcatc
      301 ctccaacatt aattgtaggg ttgtggcacc attccattga ttaacagata aagtgacagc
      361 taactccaaa tgttgtgctt gttgaaactc ttgtagttga ctccccatat taaatgctac
      421 caaatcaagt gcttgtccat cttgttctag ttttaatttc agatgagcac cgttttgccc
      481 catgactctg gcttgactca ctttaaaatc ttttaacaaa aagactggtt ttttattatc
      541 cattccaaat ggcgctaact tttcaaaatc tctaacagtg tctaaactaa gttcgtcaaa
      601 acgtagctca ctatcaattg tcagagtttt tttctgactc atgtccaatt gttttttaga
      661 gatatagtca cataaaactt gagaaagatc acctacttta ctttcctcta aagtcatgcc
      721 tgctgctccg ctgtgacctc caaaagcaat gaataattct cgatgctggt caaaagcttg
      781 aaaaatatca agtgcttcta cacttctggc acttccttta gcaataccat cttcaatatt
      841 caaaacaata acaggttgtc cagttctctc taaaatacga cccgccacaa tacctaaaac
      901 ccctggatgc caattttctt ttgctaaaac ttgtacaggc tttgtttgat caagcatttg
      961 catagcttgg tcaaagatag tttgaacgat ttctttgcgc tcttcatttt tttgatcaat
     1021 catttgggct attgcttgag attcttcatc atcaaaccct gttagtaatt caatagctgg
     1081 attagggtca tcaagtcttc ctaaagcgtt taattgtgga gcaattttaa atccaatagt
     1141 ttcttcattt agtgttttta aatcaatatt tgataaagat attagctcct gtaagcctat
     1201 tctttcacta tctttcataa cttcaagtcc agcttttacc atgatacgat tttcatcagt
     1261 taggctaacc atatctgcaa tcgtcccaat agcaaccaaa tccaacattt ctgtcgggat
     1321 tgtttctaag agagcacaag ctactttgaa agcaacacca caccctgcta aataaggaaa
     1381 tggataatta gcatcaggat gttctgggtg aataatagca taagcacaag gtaaatctgc
     1441 tggcatactg tgatgatcag taactaccac gtccacacct tgattttgag catatgtaat
     1501 tgcttcatgc cctgccacac cattatcaac cgtaatgatt aatgaaacat cttgttgttc
     1561 aataaaatat ttataaacac tttgatttgg accgtaacca tctgtaaagc gatttgggag
     1621 ataaacctgg acttcagctc ccatcatatc taatgcttct ttcatgattg atgctgatgt
     1681 cattccatca gcatcatagt ctccataaac taaaatagtt tcattatttt caatagctcg
     1741 tctaatacga ttaacagctt tatccatatc atttaacaaa taaggatcat ggagattttc
     1801 caaattagtt tgtaaaaaat gatgtaattc cgctgatgtc ttaatacctc gcgaataaat
     1861 taaggaagcc accgcttcac ttatcttttc tttttttgaa gcctcaaaaa agccagcgtc
     1921 tggcttttgg ttattgagga cccaactata cttagcagat atcatttata attgaatacc
     1981 tttctggcaa aataatcagc tgtttttgga aataaactat aatatttatg cgcaaaagct
     2041 aatataaaag gaagatttaa ttctctctta ttttttccaa gaatagaaac tactttttta
     2101 gatacttttt ctggtgttag agcaaaacga cctatacttg ctaaataatc accacttgga
     2161 tcagcctgag caaaaaatcc tgttttgatt ggtccagggt ttacacttgt cacataaaca
     2221 tttttttctg ctaattctaa acgaagagca ttcgaaaatc caacgacagc aaacttacta
     2281 gccccataaa cgctagcttt acttgttgct atgaggccag ccatgcttgc aatattaacg
     2341 atatgaccag attttacaag agacatctta tgtcctatct gacgagctat agacatcgtt
     2401 gcaaatgtat tcacagcaaa catgtctttg acttcttcat cactataatc ccaaaattct
     2461 ttaaaagaac caaatccagc attattaatt aaaatatcaa tttgaccaaa ttcaccataa
     2521 attttttcaa tcatattcgt tacagcattg tcattagtta tgtctagtga taaacataag
     2581 gtgtttggtc tctttccata aagcttttca agtttttctc ttgatctccc tgtgacaatt
     2641 aaatggtcgt cttggggtaa ttggttaata attgcctgtg ctaaaccacc agaagctcct
     2701 gtaatcagta tcgttcttgc cattttatat acctacttct tccaaatctc taacaagatg
     2761 ggtatttgaa aaaatcgtct ttgcatcttc ttccatttgc caacaatctc gccccataaa
     2821 acgagcacta acatgattta ataatagtcg tttagcattt gcttgtttag cgatatcagc
     2881 tgcttgcata ttggttgaat gcccatgcga tttagcaatt cgctcatcac ccttaccata
     2941 agtagattcg tgcactaaaa cgtcagcgcc caaagctaac ctaatacttg catctgtttt
     3001 tctagtatct cccaaaatag tgattacttt accctttttc ggttcagaaa tataatcttt
     3061 agcaataatt tctctaccat cttctaaggt aacattctca ccatttttga ctttcccaaa
     3121 aaggggacca aatggaacac ctgctagttt caatgcttca gcatctaaag tgccctcaag
     3181 gtctttttgc acaacacgat atcccataca aaagatagta tgatctagct tttctgcata
     3241 aacggtaaat ttatctgttt ccataatttt tccgagtgat gattcatcaa actcgtgaaa
     3301 atggattcga taaggtaaac gtgaacctga tgtccttaag ccagtcataa caaaactttt
     3361 tataccaacc ggtccataaa tatctaaatc tgtctgttct tcattggctt gaaaagcccg
     3421 actggataaa aaacctggaa gaccaaaaac atggtctccg tgcatgtgcg tgataaaaat
     3481 ttttttgact ttacgaggtt taatcgtggt ttctaaaatt tgacgctgtg taccctctcc
     3541 acaatcaaac atccaaacct cgttaatttc atctaataat ttcaagacta aacttgaaac
     3601 attacgagct ttcgctggtt gaccagctcc agttcctaaa aattgaattt ccatacttgt
     3661 tgactttttt ataatccttc cataacttaa taaatttaat tagtagattt agtatcaata
     3721 taaataactg actcacgtcc ttgactagaa taatagtatt tacctgactt tgttttaacc
     3781 aactctaata gctcttcttg attgaaacta gttattttaa taatatcttt tttaagttgt
     3841 aagacattcc ctagtaaact tgtttctggt gtaatatttt gagattctgt ttcgtcaaaa
     3901 tctatttttg ccattctgat accagaccga tcttgataga ctttatattg aggaaaaatc
     3961 tgtgcaattt ctaagagtaa ctgcggtgta atgacttctt taacttcaga aaaaacactc
     4021 gcttcttctt gatcatttag gtaagcaaaa ccaaatgatt ttcctgataa attcaaacga
     4081 ataaaaggtt tttcttcatc aaacgataaa tgttttggaa acaagcccaa ggctttggaa
     4141 agatcaaaat agtaatcagt tgccgcttct ggcccttgct tttgatatat ttcagcaaaa
     4201 taagcattaa tcacactagg aggtggagtg ataaaatcaa gtcgttgttg cttagagagg
     4261 ccatctagtt ttttctctag ataaacttta tctaagctcg taaaaccacc ataagtcaaa
     4321 gccaaatcaa tataatcaat cataaaattc ttccagcttc catttttgtt taggagaaat
     4381 atagcctgta atattctcgt attcttcttc aaaggtgtag gtgtcaagca atgctatttt
     4441 attgagatca tagagcttat atgcttgatt ttgatgcact ctaatcgaaa attcgtcaaa
     4501 gatatgtcgt atttcatcca caataagacg gcgtaataaa tccctactcc cttgtttttt
     4561 ggctgcaatt ctcacattag gaaaagttgt tgcattcagc tgctcagtga catccatctt
     4621 gttgtatatc gctaaacgag gaatatctat catatctaag tctttaagaa tctccataac
     4681 aactttttcg tgttcttcat gattagggtc actagcatca ataacatgaa gtaataaatc
     4741 gacatgtcga ctttcttcta aggtagactt gaaagccgca actaactctg taggtaaatc
     4801 ttgaataaaa ccaacagtat ctgttaaagt gacttgaaat tgattttgta aatagatctg
     4861 cttggttgta gcgtctaacg ttgcaaataa ttcactggct tcatattgct tatcatcggt
     4921 caaaacattc ataatagttg acttgccagc atttgtatat ccaattaatc caattttaaa
     4981 ggttgtcgaa tcaacacgtc tttctcgaac tgtctcgcga tttttttcta caatcttcaa
     5041 ttgtctttcg atatcagaaa tttgatgacg aatggaacga cgattcaact ctaactgaga
     5101 ttctcctgga cctcggcttc cgattcctcc tgcttgacga ctaagcataa taccttgtcc
     5161 aactaagcgt ggtaacatat atttcaattg cgctagatga acctgtaatt ttccttcatg
     5221 acttctagca cgcatagcaa agatatctaa aataagttgc atacggtcaa taactttaac
     5281 accaagttca gcttccaaat tagagttttg tcgcggagtc aaacgattat taaccacaac
     5341 ggtatcaatc tcatctgctt ctacaatagc ttttatctcc tctaatttac cagatccgat
     5401 aaatgattta ctatcatatt tatctctttt ttgataataa tgattaacaa catttgctcc
     5461 tgctgtctta gctaagctag caagttcttc catggacatt tcaaaattct cagtatcttg
     5521 aagttctacc ccaactaaaa taacacgttc ttgctcttct ttagtttcta tcacctataa
     5581 tttcctttca atattttttt cttgttctaa atacgtaatc tttattataa cataatgccc
     5641 tttaaatgcg agaaacggct ttatttactt tgatgataaa aaatcactga cttttcgctt
     5701 gattttttgt tgataatttt cctcaccaac cataataaat tcgacattca tacggtttct
     5761 aaaccatgtt aattggcgtt ttgcaaaacg tctggtattt tgttttagtt tatcaacagc
     5821 ttcttctaat ggtatttgct ttgaaaagta aggaaataat tctttatagc caattcctct
     5881 acttgcttga acactagggt agttatcgta tagccattta gcttcatcta agagaccatt
     5941 gtctatcatc aaatcaactc gtctattgat acgatcatag agcacctgac gatcatcatt
     6001 caatccaatt aataaaacat catacggact ttcactattt tgcaaatcat tcccaaactt
     6061 cgccaattct aatgcacgga tggcacgtcg acgattaact tgcggtataa caatggattg
     6121 ttctgtcaac ttttcaaata aatcttcatc acttagagac tctaattttt cacgataggc
     6181 cttaagagtt tcttgattga cttcgcctcc taaatgatag ccctcaaaca aactctgtaa
     6241 atataggcca gtacctccaa caataatagg gattttcccc ttagaaataa tcgtatcaat
     6301 agctactttt gcctctttga caaaatcata aaccgaataa ttctcattta cttcacgaac
     6361 atcaatcaag tgatgataag cttgttcttg ctcttcttta ctagctttag ccgttccgat
     6421 atctaacttc tgataaactt gctgactatc gccactaatg atttcgccat tgaaagtttt
     6481 agctaattca attcctaatg ctgtcttccc aaccgcagtt ggaccaacaa ctgctataag
     6541 cttgattttt ctcatgatac ttctcaattc tataaaggtt tttacttgaa atttaactag
     6601 tatttcgtta ccataattat atcatagata gaagcagagt cttcatcata gattggagaa
     6661 ttattatggt taaaaaattc gcatttgcta aaggaatagc aacaggagtc gttgcaacag
     6721 ctgctactct tgcaggtgct gcatttgcta tcaaaaaaac aattattgaa cctgaggaag
     6781 aaaaaatagc ttttatcgaa gaaaatcgta aaaaagctgc acgtaaacgt gtctctcatt
     6841 aaacaataac tcccttacta gctagtaagg gagttatttt atattaaatt gttcgcctta
     6901 aaaagaaaat ataacgtacc attactatca taacaataag agttgttgtc ccaatttcaa
     6961 agtggactag cccctgtata tcaactggta atgatagatg ctgaatactt aatttaagtg
     7021 aggtagctga gataacaagg gggaaagtaa aggctgagaa acctggtgta aaatcaccaa
     7081 tcagtaattt aggcacctga aaaagaatga aaaaataaag aaattgtgcc attacgatta
     7141 atagcaataa taaacctcta tttggtgaca caaaactatt gacatatctc gctgtgatta
     7201 gcgacattgg agcgcaaata gtagttatat taggcttaac tgccgaaggt agcccgattt
     7261 tatatgcttt aatgaataga aatggtaata atactaaagt tgcaataaaa ccataccaaa
     7321 aaactatttt cccaatagtg aattgtccac taattggtgc tgtcaggcta gctaccccta
     7381 taccaacata gaggacagac caagaaggaa aaagattagc catcgaaaag tgtctcaaat
     7441 atttccatga aaaaatagcc atatgaatga ttaacgcaat aaatgacaac caccatacac
     7501 cttttgacag aactgctacc catattccta aacttctaaa taataagata taggtcgata
     7561 aaatcatacc tgccataaaa aaagtagtaa agacagaagc aatcaaagga ttgcttaact
     7621 ccttcctgct ttctttttta ttttttaata ttcctttgat taaaaaaatc caaaaaacaa
     7681 gtgctactag ccctaaacaa tagcgaaaat aggtgccata tccctctaga aggttcccca
     7741 aagctaatag ccccaatact aatcccgcca tcactaatgg tggtttttcc caatttttca
     7801 tcatatacct cataaaaaaa cgtttacatc cattataaca caattagatt ttgctgataa
     7861 gtgattttat taacttaatg aaaagaagct agcccattga ctaacttctt ttttaagctc
     7921 ttctaaagtt tgcttttatt ttaagtgacg attcctcttt aaagagagac ctagtagagc
     7981 aagtagactt gctagaatgc ttattcctac tacacttagt atttttgatg acttatctcc
     8041 agtttttggc aaattagctt catttgtcag atatgaatcg gtcttattag aaactacagg
     8101 agttattgag gtcttattag ctattctatt tactttttct accgttttag atcccttgaa
     8161 agattgtgtt ttgggataat ctgcttgcaa ttcctcctta gggctaggag caactttgcc
     8221 aactttaatc acaattgctg ttaatggatc aatcagcaaa ctatcttttt caaaatgaac
     8281 acctctagga gttgagattg gtttaatccc cgcttgatca gcatcaacaa ttacttgccc
     8341 ttttaaaagg tgtttatatt tttcacctag taaaacgttt ctagctttac tatcagcatt
     8401 aacaaatact gcgtaaatat cgcctttaga atctattgtt tggtaagcaa taaccaaatc
     8461 tttttcttta atatcacctt gacctacctc tgtaatcaag ctaacctcac gatcaatttc
     8521 tgctttgctc aatttccgga aagcatctgt tgaacgcctt aatgtgatta aacctgctgt
     8581 ataggcctgt gttttcgttg aaattgggtg tttgttatta tctgtggctg ctgcccaatc
     8641 aaaatgatta atggcatctg aagaatcata tgaatcatga ataaaatatg gatattcttt
     8701 aacagcttca ataagtgttg ctttattagg caatttgtca tctgaaactt ttgtcatgta
     8761 atcagggtta agtaaacgct tcgtacgacc atactcttga ccagaatgaa tgaatgctgt
     8821 cccttgagat gttaaaatca ttacatttcc taaacgcaga cgtctatgaa tctcttcttc
     8881 agctacctta gggtctttat taattgattt tgcaatcaca tcatgcaagg taaggttatc
     8941 atgtgcagca atatactgca ccacatctcc tggcgaatct gcttcaaaat tcccaggttg
     9001 tgctttgata tttttaaaaa taccttgtaa agattgtggg ccacctgtga tgaaagctgg
     9061 agtaccttca tttggaaaac cagatttcaa gctattacga atatcatctg aaaagacgcc
     9121 aactgtatcg gttgacttca tccaatcttg gtcagctggt ttaaccggct taccttgatc
     9181 gccttggaat gttctccagc cctcaccaat cataatcata ttaggattaa tagctttagc
     9241 ttctttataa gctaattcaa tcgcagccgc atcatgatct cccatcatat caaaacggaa
     9301 accatcaact ttaaattcac ttgtaagata tttaatggaa tcaaccaaaa cacgacgact
     9361 cattgcatga gtggttccta aacgtccccc tccaaaactt tctcttggtg aaccatcttc
     9421 attcataaag tgataataat taggttctat atcctcaaag agataagttt ttgcagtgtg
     9481 attatagacg acatcaagta taacccccat gccacgttta tgaatatcat gtattaattg
     9541 ttttaattcg gcgatacgtg ctgatggatc ttttggtttc tctgaataca ttccagaaag
     9601 agcaaaatag ctctgtgggt catagcccca attgtaatta ttgtctgagg aagtgtaagc
     9661 tgttgagcgt gacttatcca tttcattaac ataaaaataa ctcaataccg gtaaaagctg
     9721 aatgtgtgta actcctaatt tctgtaaata atctagtttc tctgaaaagg ctgcaaaggt
     9781 aacaaattga ttttttaatt ttccgtccaa agattgatca gaagtgaagt ctcttacatg
     9841 tgcttcgtat ataacagcat cttgtcttcc tttaaaatta gcaattttag caaaacttaa
     9901 attttgaggt ccaagttgac ttggatttac aaaagctgct ttagccgttt ttatatcgtc
     9961 attaacagta ttactatccc actctgctaa tgactttgca taaggatcta aaatcttaac
    10021 cttatcctta cctcttttta tttcgtaaag ataatagtaa ccagtatagt ttttaatacc
    10081 taatttagta tcaagtatcg tctgccaaac acctttatta tttttcacaa ggggggtagt
    10141 cgctacaacc ctgttttgat tatctttgtc ataaataatc atagtgacac tatcagcact
    10201 cggtgaccag aggctggctt caacttttga accatcttga ttgagaactg cacctaaatt
    10261 tccactataa gcataaagtt ggtctttaaa ttcccaagat tgccttgtcg tgacattgtt
    10321 accattataa gatatattga aatgaccttg tttaggatta aagtcgcctt tgattattaa
    10381 aagagattta ctagtatcga gagtgatatc agaaatttgt atagcatttt gatttttatc
    10441 tgtcactttc aattctttta aaatttcagt tttatctacc ccatctagag ttgtaaaact
    10501 tgcttgaata cttgttaaat cagtttgttg agcatccttt agctgcactt gatcaatgta
    10561 ataaggatta ttataaacct ttggatcctt atcttttaca aaaatttggt tatggttagc
    10621 taaatctcta aaaacatagt cgttgggttg aactttcact gcatctcctg tcttactttc
    10681 atctaagatt agaaaaccaa tctctttggc attagttttc agtggtacat caatatacct
    10741 tccatatagt ccttgattca caaaattact accatctggc caagttgttg agggggttgc
    10801 aacatcttta aagagccatg ctgataagtg gtcatagtta ccagatgaac tcagatagtt
    10861 aatacggaca tacccttctt tgaggggctg ataagtatgt ataccgtact tttcatcaat
    10921 ccaaacttgg ttcatctcag gtcgtaataa tggaatatga tgatcgccgc ttaaatttgt
    10981 tcctgctttg ttattaatta aaaaagatat ttgttttcgt tgtttttcag ataatttaaa
    11041 atcaacataa taaccgtaat catctttctt agcattagtc atagggatag caccatttgg
    11101 ccaattacta gacggttgat caacatctcc ccatatccaa agcccagtgc tttctacagg
    11161 atgaccttga ggcaattctt taagatgtaa cctaaagtaa ccattttcaa tctgtggagc
    11221 ttgattagca actttcaagg tcggacttgg agcacctata gcttgagaag ttttgtcggg
    11281 agtattttgc aatgtagatg gtgccacttt taaatctaag gtatttttat ctgaaacagg
    11341 acctttattt tcttcaagca ggcttgtcgc cttatccggc tctgtcactc ctttttgagt
    11401 gtcagtttca tctgatacta attgcttggt atccggcata tctgagggtg tcacaaccgt
    11461 ctcagagttt gagtttatgt catcttttaa attagtagat tgtcggttgt caacctgatt
    11521 aatattcgac tttgcaatcg ctgtagttgc aggagaaaca cttacttctt cagcaaatac
    11581 ctgtggacta tgaagaaaaa tacatacccc tgttgcaact gaagctactc caacacttaa
    11641 ctttctaatc gtgtattgag tttgtttatc accaaataag tcttttcttt tcatatcatt
    11701 ctcctttttt aatgaattgt taccaataaa atcataacaa tttacgcaag cgcttgcaac
    11761 aagtaaatga tttctttatt tttttgaaat tttcactaaa actatcaatg acattacaaa
    11821 aaagctaagc aatattgcct agctttttta tcaaaaattt cctcatatca aatgattttc
    11881 atcaatttga tagtccttta gcagacattg aagtctatca aattcataac aaaaatgttc
    11941 aggaatatga ccgtcagagc ctatagagta ttgtttacaa ccaagctgtt ttagtatctc
    12001 taaagcatag cgataaagtt tttcattccc atatagataa agggatttgg tatttagttc
    12061 aaaagctaac cccttagata acatctttct gaaaatgcgc ttcaattgcg tttcaaacgg
    12121 ttttaaatct tctacatcta agtttaactt acgaaaacca taatcaaagt gatctaaaac
    12181 gtgcgcttcc acacggccta tggcaaattc catacgatta aagtaatctg ctaaaagctc
    12241 ctttgttggt actttcagag cttcttcttg cagataatca tacctaccat tatgatggat
    12301 tgacaatagt tttaaatcaa attctttatt ttttaaataa tctaaaatat ctgattccct
    12361 atctttaaaa taaccgattt caattccttt tttaaatcga tttccatatt tctgattaag
    12421 ataatctatt ttttgacaat aagcactata atcaggaaca tcgtcttgac cggtgtaagg
    12481 atttgataaa tcaaaatgtt ctgttgtgat aaattcacct gtaaaaccat taatatagtc
    12541 ctcaaatgcc gtttgacaat cataggaaaa atgcgtgtgt agatgattat ctctcatact
    12601 aactcctatc cttaatcttt taaaatatct gctactatct tttgtaaatc aacgattaga
    12661 tctttttcaa accatggatt cttctttagc caaatttgat ttcgcggtga tgggtgaacc
    12721 aggggtaaat aatcgggtag atagtctttg taagatttaa ctgtttctgt tagattttta
    12781 tgtgcagagc ttccaagata atatttctga gcatattgac caactagcaa ggtcaattga
    12841 acattaggca tttcttttaa aataagaggg tgccatttct ccgcaaaacc ttttctaggg
    12901 ggtaaatctc ctgattttcc tttgcctggg taataaaaat ctaaaggcaa aacagcaaat
    12961 tttccagaat ggtaaaatgt ctcttcatca actccaagcc actggcgtag acgatctcca
    13021 gatttatctt tccaatagag tcttgcttct tgagttttta aaccaggtgc ttggccaaca
    13081 atattgatcc tagctgtttt aggagcagca aacaaaggat caataccatt ttctgtataa
    13141 ttttgattct gtgaatcact ttttattgct ttaataattt tttccaagtc agtcataaag
    13201 tctccaaatc taaccttatg aaaagaggca aagacacttt catgtattca ccacccttta
    13261 tcattgatca catatttagt ttaaggatta ttttatttga ttaattcata gatagcttct
    13321 gcgtagatag cagccgaacg gaaaatattt tctaaaggca tgtactcatt agcttgatgc
    13381 atagtgtttt catctcctgg gaacatggca ccgtatgcaa caccccgttc aagtaagcga
    13441 ccaaatgtcc caccaccaat aacctgttca tgtcctttaa gaccagtttg cttttcatag
    13501 acagctagta aggttgatac taattcatcg tccataggta catagtgtgg tgtgtgttca
    13561 tggtcagaaa gagtcacttt ttcaactccg ttaagtttct caagcttagt ttggatagtt
    13621 ttagcatctg tcccttgtgg gtaacggaaa ttaagagcga tagtattatc atcattagtt
    13681 tcatcaaact ggaagacacc tgcattcatg ctcaacgatc ccatacagtc atcttcataa
    13741 gctactccaa gtttttcacc tgagaagtct tcgtgaataa tgttggctgt gacatctaag
    13801 aaagcacgag caggaccttc aaaatcaaat tgacttagat aaagagctaa ataagtcgcc
    13861 ccattaacac cttcttgtgg tattgaacca tgagcagatt taccataaac tgtagctata
    13921 tactgcccat cttctacaga taattctgtt ttcaaattat gtttacctgc aaaatcagct
    13981 agactagctc caagtgttgt ctgatctaaa tgacttgtga aacgtgctgt ggctgattct
    14041 ggaaccatgt tttcgcgaag tccgccagaa aagctatgca gtctaacagc tcccttattt
    14101 tctcctgaaa aatgtaaata ttcagtgata ttaccttttt caccatttat aattggaaat
    14161 tctgcatctg gagagaaacc aaaatcaggt tttggaagac caacatgttc gaagtagtaa
    14221 tccatatctc cccatccaga ttcttcatca gttcctacaa caaaacgaac tttcttagat
    14281 gttggtagcc caagctcttt aataattttt agagcataat aacatgccat tgttggtccc
    14341 ttatcgtctg atgaccctcg agcatataaa cggttatcct tgataactgg ttcataaggg
    14401 tctgtatccc aaccgctgcc tgcaggaaca acatctaaat gcccaaaaat acctagttct
    14461 tcttcgcctt gaccaaatgt aaagtgtccc gcataattat ctacgttttt tgtttcgtag
    14521 ccatctcgct cagccatctc aaggaaaaat tccaaggctt ttacaggtcc aggaccaaat
    14581 ggatgctctg catcagcttg tgaatcatca cgctccgagt taatacgaag aagatttatt
    14641 aaatcgtcca tcaaagcgtc tttgcgtttg tctacttctg ctctaaaatc aattgtcatt
    14701 acggcaactc cttctataaa atattatgta gtttgtgctt aaaaattaac gtttttcaat
    14761 aatatgatca actggcaaac gataactagg ctcaaccttt tcatcactat aaccaacagt
    14821 aattaatatt tctggtctaa aacgtttatc tatctctaag acatcattgg taatcgcttt
    14881 atcgaaccct aaaatcatat tagaactaat accttgatct gtcaaggcaa gtactaaatt
    14941 catagcaacg atacctgcat ttaaagagag atactctcca gtttgttttt cactatataa
    15001 agcatagcgt ggtggtagag tttccatata ataaccaatt aaatcatcag gaagagatct
    15061 acgaccaata cgagctattt tccgtgatct ctgtcctaaa tctgtatctg taaataaagc
    15121 aataacatac tgcgcttgat taatttggtt acaattactt tccggtaaac cttctgctag
    15181 agcagatttt ttttcttgga caacaacaaa cttccaaggc tgaatattat tagcagatgg
    15241 cgctaatgtc gcaatttcaa tagcagttct aacatcttta aagtcgactg gcttatcatt
    15301 aaaatgtttc acagcatggc gcttcttatt taattctaga aatttcataa aaaacctcct
    15361 attctatttg attttcccta ctattctatc actttttaaa atacgtttca gcttttgaag
    15421 gcaaaattga taaaaaagtg aaaccggttt gaaaactacc ataaaaacaa acaaacttgt
    15481 ggtacaatat ctaatgacta taaaaaagga gctattcatg ctacgagcat taaaagaaga
    15541 tttttttcta accttaacct ctttaaaaaa tcaattttgg gtttacattg gaactggtat
    15601 cttaatccaa actttagttg cttatttagt aaaaggaatt ctgagtttta tctttcgtcg
    15661 gatattgata ttatcaaata ctcccgcagt gactaaagac aattggactt tgctttttaa
    15721 tcatccctta gctttattac tctttatact ttatatcgta atcctaattg ggttcatcta
    15781 tactgagttc actatctata ctattgccat actgaaaact gaattttctc tcagtatcaa
    15841 aaaatggcta acaatctttc ctaaaaaaat aaaatcactt ttaggaccac agctaatttg
    15901 ggtatcaatc tacttacttt tgacaattcc tctagctaac cttggtctaa ggtcaagtat
    15961 actagagcat cttaaaattc ctgactttat ttctggcgag ttaacaaaga cagtctttgg
    16021 aaagataggt tactctaccc ttttactgct tatttgttac ttaaaccttc gattaattta
    16081 ttttctcccg ctgaccattt taactgacta caatgctaaa gaagcacttt tagagagttg
    16141 gaaacttagc cgcggaaagc gtcagtggcg tcttctaagt aaaattattt taaccagttt
    16201 atttatatct gtaatcggaa cagttgcact agctgttgtt gctggtacac tttcattcat
    16261 tgatcattct ggaaacaatt ttcctcttca agccactttc tataaccttt taaaatcaat
    16321 actttttgcc gtaacacttc ttattaaatg tttaatcata agtcacctat tagcagatat
    16381 taaacaaaat aaaagtattg ttcaacaatg gagagcttgg aaaggcgtcc accataagca
    16441 aaaatttaaa cacattgctt gtgttattct agctctagct attggtacca ctgccttaaa
    16501 aaatgctata gctctggctc tcttacaaga taacatttca attaaaaaag aattaatagc
    16561 ccatcgtggt gatacatcac atggcgttga aaattctatt gaagcattaa aagtagccca
    16621 taaagcaaaa gcagattatt cagagatgga tgttatcatg acaaaagatc ataaattagt
    16681 agtcattcat gatgataatc tcaaacgact ctctggtatg aacaaagatg tttctaaact
    16741 aactttagat caagttacaa aaatcccaat tcaccaaggg cattttgcta gttacattcc
    16801 atcatttgca gaatttatga agaccgctca gtcactagat caaaaaatca tgattgaatt
    16861 gaaaccgtat aatcaaaacc tcgatatcta tgcggatgaa tttatcaaag aatttaaaga
    16921 gcttcgtcta tcaaccaagc ataaagtaat gtctctcaac ttgacactca ttgaaaaggt
    16981 tgagaaaaaa ttgccacaac ttgatacagg ttacctaatc ccactacatt ggggaacgct
    17041 tcaaaatcat aacgttgact tttatgggat tgaggaattt tcatacaatg attggattgc
    17101 atatctcgcc caagaatata ataaacagct ctatgtttgg actattaacc gtgataacct
    17161 tatgatacgt tatttacaaa gcccagttaa tggaatcatc acagataaac tcaatctttt
    17221 caaggtcatt aataaagata tcaaaaatag ccctaattat tatcaaagag ctctccaact
    17281 tattgatagc gagggttaaa ttaaataaaa agcttagtag atactgttct actaagtttt
    17341 ttatttagga ggatcattaa gagaatctcg gagactttct gcaactgtcc taggaatacc
    17401 acacataata atgtcctcaa tatcagcttt ttggatatta ggtaagcttt taaaatgttt
    17461 cattagcaat tgtttacgtt ttggtcccaa cccagtgata ccatctaatt ttgaagaaaa
    17521 ggaatttttg gagcgtaatt gacgatgaaa agtaatagca aaacgatgta cctcatcttg
    17581 aatacggtga agtaagaaaa attcttctga attacgcggt aattctacta cttctaaagg
    17641 atctccaaaa agtaattcat gtgtttggtg tttatcattc ttttgcaaac ctgcaatagg
    17701 tatggctaaa cctaattggt tctcaataac atcacgcgct atatttactt gcccctgacc
    17761 accatcaatt acaatcaagt ccggaggtgt caagccatct tttaaaaccc tactataacg
    17821 ccgatgaatg acttctctca tactagcata atcatctggt ccgataacag tcttaatttt
    17881 aaactttcga tagtcttttt tacttggctt tccattcaca aataccacca tagcagctac
    17941 tggacttgtc ccttgaatat tggaattatc aaatgcttct atgcgtactg gtttagggat
    18001 attaagaaga ttacctaaat tttcaattgc cccatgagtt ttacgaatat ctttttccag
    18061 taagtcaaac ttctgttgta aactgacacg agcatttttt attgccagat ttactaactg
    18121 tttcttttca ccacgttgtg gtttaacaat cttacagcct acaattgttt caaccgactt
    18181 agcatcaata tcctgtggaa taaagacttc tttgggtaga aaatgttttg tatcttgata
    18241 aaattgccct atgtaagtta gaaaatcttc ctctggttca ttataataag gaaacatatt
    18301 cacgtcacgc tgaatcaatt ttccatttcg aacaaaaaag acctgtacac acatccaacc
    18361 cttattaacg aaataaccaa aaacatctct atccttcata tcttgatgaa taacacgctg
    18421 cttagtacgc aacaaactaa ttgcttcgat caaatctcga tattcagccg cacgctcaaa
    18481 ttccatagtc atagcagcgc tcttcatttt ttcagttaat ccgttaacaa ttttattatc
    18541 tttaccatta agaaactgtt ttacatcttc tctcagacta tcccaataag ctttattagt
    18601 ttgacaaact gtatgtgcat tgcattgccc taaatgataa taaaagcata ctttatttgc
    18661 tggattagta catttcttaa aaggaaagag cctatctagt aaccgcttaa tctcagttgc
    18721 tgccccagaa tccggataag gtccaaaata agtgccatct gattttttta cttgacgtgt
    18781 aatcataaga cgaggataac gttcatttgt tattttgata tatgggtaag acttatcatc
    18841 tttcaatcga atattatact tcggcatatt ttcttggatt aaattaattt ccaataatag
    18901 agcttcggta ttagatgttg tcacaatgta ttcaaagtct tcaatctcag agaccaaaag
    18961 ttctgtctta gtattatgac tcccatgaaa ataacttttc acacgattct taagattttt
    19021 agccttacct acataaataa ttgtcccatt cttatcttta tgaaggtaac aaccaggact
    19081 gtctggtaac aattctagct tatgtttaat cagttcattc atagaactat tataacaaaa
    19141 tgtactgtaa tctttacgaa ataaatacta ttccccaaca tctattgtat cttccaatac
    19201 ttgaataaaa ccccaatact cctagtctaa gcaattattt tatgttacac tttaaacaag
    19261 tgaattattg aggagttttt atgatagacg ttgagaatat tcttgaaaaa atgaaaccta
    19321 accaaaaaat caattacgat tgggtcatgc aacaaatggt taagcagtgg caagctagtg
    19381 atatccgtcc gaaaattctg atgcatgttt gctgtgctcc ttgtagtaca tatacccttg
    19441 aatacttatc acaatgggca gatgttacca tctattttgc taattcaaat atccatccta
    19501 aagatgaata ttatcgccgc gaatatgtta ctcaaaaatt tgttcatgat ttcaacaaaa
    19561 atactggata ttctgtacaa ttcttatcag caccatatga accaaatgaa tttttcaaaa
    19621 ttgttcatgg gttagaagaa gaacctgaag gtggggatcg ttgtaaagtt tgctatgatt
    19681 tccgtcttga taagacagcg gaaaaagccg ttgaactagg ttttgattac tttggcagtg
    19741 ccctaactat tagtcctcac aaaaactctc aaacaatcaa tactattggt attgatgtcc
    19801 aaaaaattta tgatactcag tatcttccta gcgaccttaa aaaaaataaa ggttaccaac
    19861 gttccgttga gatgtgtaaa gattatgata tctaccgaca atgttactgt gggtgtatct
    19921 tcggtgctaa agatcaaggg ataaatcttc tccaaatcaa aaaagatgcg aaagctttcg
    19981 tgagtgataa agacggcaaa gaagaatttc ctaatatacg cttcacattt aatggcaagt
    20041 caatgtaaat gataaaaaaa gacttagtaa agaactaagc cttttttggt ataaatcttg
    20101 tcaaacttta ataatacaac ttcttaatct tttgcagacg ttggtttaga aatagaccac
    20161 aaacaaaaat acctgtgatt aaaccaatcc aataggcaaa cggacctaac tggctatttt
    20221 tttctaagat aaccgctaat ggcaaagcac ataaccaata agagcccgca ccgatcataa
    20281 atggttttgt tgtatcctta tagcctcgta aaatcccctg tacaggagct gcataagcat
    20341 ctgcaaactg gaaaaagaga ctataagtta ggaattgagc tgtaatagcg acaaagtgag
    20401 ggtcactatt atacattgct gctacattct cacgaaatag aaataaaaat agtaaggttc
    20461 ctgatgtaat ccctaccgct gttaagcgtc ctatcctact ataagtgttt gcgtcctgaa
    20521 agcgctctgc ccctacttca aacgatattg taatagctag agcagtggaa atacttaaag
    20581 gaaaggcata cattaatgat gaaaaattca tagcagcctg atgtgctgca ataatgattg
    20641 aagaaaattt tgccatgaat aagcctacta ctgcaaaaat tgcaacttct gcaaaaattt
    20701 gtaaaccaat cggtaatccc aatcgaatat cttcaataat caaaggagct tttattcttt
    20761 ccagagtcca tatatgatat gttttaattt gaggatgaag tgacatcaca ataataataa
    20821 caataaaaat agcccaataa gttaaagaag ttccaagacc tgcccccgca cctcctagtc
    20881 taggcatacc aaatttaccg tagataagca tataattaaa aaatgaatta aagggtagaa
    20941 ttaaaagcat cagatacata gataaccttg ttaaccccaa tgcatcaaag aatgaacggc
    21001 aaatgctaaa caacaccagc ggcatgattc caatcaacat ataatttaaa taaccacgac
    21061 caactgctag aacttcatct tctaaaccca aactccccaa gacaggttga gcaataaatt
    21121 gcatgattaa aaataatatt aaggacagta tcaaacctaa atatagaaat tgatgaaatt
    21181 ctgtgcgaat ttgttcttta tttcctctac ccaaatgttg accaactact ggtactaatg
    21241 ctgaaatcat acctactaat aaagcgaaaa acggagtcca taaattacta gcagttgaca
    21301 cacctgccaa atgtagctga ctatactgtc cagtcataac cgaatcaata aaagtagctg
    21361 aaaaattagc aaattgataa atcaatactg ggataaataa tttcaaaaat aaaacaaatt
    21421 tttccttatt tgtctgagtt tgatacatct tttttctcct tttcacctta atcaatttat
    21481 tttagcttag ctaggatact ctgtcaatgc tatttaaaaa ggttgagaaa taatcctcaa
    21541 ccccatttta gctaagctca gtattgattt gccatgtcat accatatttg tcagtcacat
    21601 ttccataagc tggactccac ggaactgctt gtagaggcat tttaatgttc attgcatctc
    21661 gagaaagctt attgaataac tcttgcgctt cttctacaga ttccacaatt aaagctgctg
    21721 tcatattttg tccaatttga aaatcaaaaa taggattatt gtcagaaagt tgcattcgaa
    21781 taccatcaat attaaattga gcgttcataa ttaaatgttc aagttctggc ggacaatctg
    21841 gccaaccctg tgcaaaagtt gtgacgtgtt ctgcttttgt attaaaagcc tcttcataaa
    21901 atgtaatagc ttcggcccca cgaccattaa tattcatata aatttcaatt gctttcatta
    21961 ccatacctca cttcatatta tccaaccaac gctgcatcag agtttcattt tcccctttta
    22021 gggtaaaata agaaccacgt attatacctg cgagttttga cttttgacga gcaaacttaa
    22081 acttgtccgc taattcaata ggaatatcca ttccatctga taattttaaa cctacagctc
    22141 tctttttagg attatctaat tcatataaaa tattaatggc taaaccatcc tcatagaaaa
    22201 catgtgtcca taaaatacga ttctctaaaa aaagctgtga aatctctaaa ggctttgcag
    22261 caaaaacaag gttacgttca ttttttagaa ggtgattaat atctgccatc aattctggat
    22321 agtctgatac tggaaatgtt tcgtaggcta ctttgaattt attccagaaa tagcgagctt
    22381 catttgcacg caaaccagcc aaggcttctg ccactggaga agtctccaaa cctttagttg
    22441 aaacggtttt aaagtcgata atcatgttct ttcctttttg attaaaatct tcgataattt
    22501 agtatactct tttttgaaga aaaagtacat tctaatcact tataaaaagt agtacattta
    22561 gaacactacc acctcagtaa tttcttgacc tttctctact taatacttat aattaatcta
    22621 gacttaatgt caaagcatca atcaaggtgt aaacctgatt cacctaaaaa gctgatggag
    22681 ctaaacatga ccaaaaagat attcttattt atctgcgcta ctcttttagt aggaatagcc
    22741 ttatatacac caacaacttc tatttttaat cataacaacg tttatgctgc caccaagaaa
    22801 gcaaaaatta aatttaataa aactcaaaaa aaaattgtca aaaaagcaag agaatatgct
    22861 aagtctggcc atatgtctaa agatagtatc attgaaaaat taaaaaagga ttctaagaaa
    22921 tatcgtcaag aagacatcaa ctttgtgatt aataacctca aggttgatta caaaaaaaat
    22981 gccttgattt ctgccaaaat ttattcaaag accatgaatt tatctaaaca atctatcttt
    23041 gaacagctct attcggagtc tccagataag gcaacccact ctgataaatt tacaaaagaa
    23101 gaatcccaat atgctataga ccatctcaaa gtagatttca aagaaaatgc tcttgagact
    23161 gctaaaagtt atcaaagtag ttcttcttta tccaaagaag aaatctataa acaattgaca
    23221 tctacgctgg gcgataaatt cacaaatgat gaagcccaat atgctgtaga tcatttgaaa
    23281 tgatgacata ccaatagtct atccttatgt aatagaattg atagcttatg ctaatattcc
    23341 agctgtaaaa tagcaaaaac cctcctaagt tcattaagac ttaggagggt ttttatcaat
    23401 atgaataggt tacactaaac tagacagaaa ttataaagtg ttctacactt aagaaaaact
    23461 ggagaaaaac catgtctaga aaagtacgtc gtcacttcac cgatgatttt aagcaacaaa
    23521 ttgttgacct ttacaatgtt ggtagaaagc gtagcagtct catcaaggta tatgagctaa
    23581 ccccttccac cttcgataag tgggttagac aagccaaaac aactggttca ttcaagtctg
    23641 ttgataatct gacagatgaa cagcgggagc taattgaact cagaaaacac aataaagaac
    23701 tcgaaatgca attagatatc ctaaagcaag cagcggtgat tatggcacaa aaagggaaat
    23761 aatcactgct aataaggata aatacagcat ttcaaagatg tgtcgctggc tgaatatgcc
    23821 acactcaagt tattactatc aagccgtgga gtcagtatct gaaacggagt ttgaagaaac
    23881 tattaaaaga attttcctcg atagcgagtc cagatacgga tccagaaaaa tcaaaatatg
    23941 cttgaataac gaaggtatca cactttcacg tcgtcggatt cgacgcatta tgaagcgact
    24001 caatttggtt tctgtttatc agaaagccac cttcaaacca cattctagag gcaagaatga
    24061 agcccctatt cccaaccact tagacaggca atttaagcaa gaaagaccac tacaggcctt
    24121 agtcactgac ttaacctatg ttcgtgtagg caatcgttgg gcttatgttt gcctcatcat
    24181 tgacctatac aaccgtgaaa tcatcggcct gtctcttggt tggcacaaga ccgctgaact
    24241 cgttaagcaa gccatacaaa gcatccctta cgccctgacc aaagtcaaga tgttccattc
    24301 agatcgtggc aaagagtttg ataatcagtt aattgatgaa atattggaag cctttggaat
    24361 cacacgttcg cttagtcaga ctggttgtcc ttatgacaat gccgtagctg aaagtacgta
    24421 tcgtgctttc aaaattgaat ttgtttatca agaaaccttt caattgctgg aagaactagc
    24481 tcttaagact aaagactatg ttcattggtg gaattaccac cgcattcatg gtagtctcaa
    24541 ctaccaaaca ccaatgacta aaagattaat tgcctagaaa aacactttat aaatgttgta
//
LOCUS       DATRMA010000024        23622 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.024, whole genome shotgun sequence.
ACCESSION   DATRMA010000024 DATRMA010000000
VERSION     DATRMA010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 23622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23622
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.024"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            612..1403
                     /locus_tag="VBC28_000923"
                     /pseudo
     CDS             612..1403
                     /locus_tag="VBC28_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743858.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1470..2015
                     /locus_tag="VBC28_000924"
     CDS             1470..2015
                     /locus_tag="VBC28_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6814924.1"
                     /translation="MKKITTLILASSLLLVATTSVKADDNFEMPTRYVKMSEKSKAFY
                     QRLQEKQRKAHTTVKTFNNSEIRHQLPLKQEKARNDIYNLGILISQESKGFIQRIDNA
                     YSLENVSDIVNEAQALYKRNYDLFEKIKSTRDKVQVLLASHQDNTDLKNFYAELDDMY
                     EHVYLNESRVEAINRNIQKYN"
     regulatory      2166..2255
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            2411..2977
                     /gene="thiW"
                     /locus_tag="VBC28_000925"
     CDS             2411..2977
                     /gene="thiW"
                     /locus_tag="VBC28_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy coupling factor transporter S component
                     ThiW"
                     /protein_id="HEO6814925.1"
                     /translation="MTNHKTKKLTVLAIMIALDVFLTPIFRIEGMAFMSSVVNIIAGV
                     IMGPIYALVMALVTAFIRILTQAGVASVAPLAILGAGPGAFLAGWFYRFYAKDWMSWV
                     GEFIGTGIIGALLSAPIMVWFWTWTADGNHEVLAKASAAQSIFLFMPRFIGATIIGGV
                     IALLLLQGLKRVPVFMTIQALFKGEKVK"
     gene            2974..3528
                     /locus_tag="VBC28_000926"
     CDS             2974..3528
                     /locus_tag="VBC28_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEO6814926.1"
                     /translation="MKLSLKDVLLIALLAVVLGVVYFGAGYISNAFVPFVGPIAHEVI
                     YGIWFVAGPMALYILRKPGTAIVAELLAALIEVLIGSIYGPSVLVIGTLQGLGSELGF
                     TLFRYHNYKLPAFILSAILTSIFSFAWSFYANGLSAFSFSYNILMLIVRTVSSIIFFL
                     LTKNICDQLHRSGVLNAYGIHERK"
     gene            3532..4818
                     /locus_tag="VBC28_000927"
     CDS             3532..4818
                     /locus_tag="VBC28_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000953609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO6814927.1"
                     /translation="MLSVEKLACTHGDSHYLFDEVTFSLNPGERILISGYSGCGKSTL
                     ALLLSGLKESGKGQVLLNGSLIEPSDVGFLFQNPDLQFCMDTVAHELYFILENLQIEP
                     EQMQDRSEFVLAQVGLKGFQNRLIYTLSQGEKQRLALATIFLKSPKLIILDEAFANLD
                     QESASQLLQLVLNYQANNQSMLIVIDHLITYYQDIMDHYFWLEKRLTRVNFDYMLNRL
                     NVFELEKKSHNTGDKLLSIKDFQVKLSKNKFISYLDFDLASGERLCLDGPSGVGKSSL
                     FMGLLGLYRTKGKKQFTHRKQIPISFLFQNPLDQFIFSTVYDEIFQVCKDSNKARDIL
                     ETINLWDKKQFSPFQLSQGQQRRLAIGSILASDSKLLLLDEPTYGQDAYHANMITTLL
                     LSYCHKNHCGVIFTSHDPHLKECFATRFLEVSSCSI"
     gene            4806..5474
                     /locus_tag="VBC28_000928"
     CDS             4806..5474
                     /locus_tag="VBC28_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HEO6814928.1"
                     /translation="MFNLKNYHPAITFLCLLIPTFSFSFTLRLRTSLLFLIIVVTLQC
                     FVKVSLKTWAKVNLISFVMGLSLFLGTYFWGKLPHQFVLASLVACRPLIFMNVGLLFH
                     ASHSNYDFIESLYQTFKVPSHFAYGIFAVFNLLPLIKLQYQRNRLAFRLKNQVTWALS
                     PRLILSVLLKTIYWVEQLELAMLSKGFEVGKERTHASTYPVRFRDYSLLGMSILLSIG
                     MIFK"
     gene            5558..6238
                     /gene="tenA"
                     /locus_tag="VBC28_000929"
     CDS             5558..6238
                     /gene="tenA"
                     /locus_tag="VBC28_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiaminase II"
                     /protein_id="HEO6814929.1"
                     /translation="MLKDKMIAKGAKYIQSIYQDPFIQGIIKGRLDHDVICHYLQADN
                     IYLGKFADIYALCLAKSDNLRDKQFFLEQIDFTLNRELADGEGPHQALAAYTNRSYQD
                     IIEKGVWYPSADHYIKHMYFHFYENGIAGALAAMSPCPWIYHQLAKKIIEENQFLNGN
                     PFNNWITFYANDTVEELMENYFRMMDYYAQNLSKEKQADLVDAFVKSCQHERRFFQMA
                     INQEKWED"
     gene            6240..7037
                     /gene="thiD"
                     /locus_tag="VBC28_000930"
     CDS             6240..7037
                     /gene="thiD"
                     /locus_tag="VBC28_000930"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEO6814930.1"
                     /translation="MTYLAPVLTIAGTDPSGGAGIMADLKTFQARRTYGMAVVTSVVA
                     QNTCGVRGVQHIETAIIDQQLACVYDDIKPKAVKTGMLAERETISLVASYLKKYPQPY
                     VLDPVMVATSGHRLIDSDAVEALKEDLLPLATIITPNLPEAEVLVGYDLSDEVSIIKA
                     GYDIQKQYSVRNVLIKGGHLDGLAKDYLFLEKEGLITLSNQRINTIHTHGTGCTFAAV
                     VAAELAKGQSILNAVSTAKSFITSAIETAPELGLGNGPVNHTSYQGD"
     gene            7039..7809
                     /gene="thiM"
                     /locus_tag="VBC28_000931"
     CDS             7039..7809
                     /gene="thiM"
                     /locus_tag="VBC28_000931"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HEO6814931.1"
                     /translation="MYLEQLKEVNPLTICITNNVVKNFTANGLLALGASPAMSECIED
                     LEDLLKVADALLINIGTLTKESWQLYQEAIKIANKNQVPVVLDPVAAGASRFRLEVSL
                     DLLKNYSISLLRGNGSEIAALIGEKQASKGADGGKVADLESIAVKANQVFDVPVVVTG
                     ETDAIAVRGEVRLLQNGSPLMPLVTGTGCLLGAVLAAFIGSSDRSDDLACLTEAMTVY
                     NVAGEIAEKVAKGKGVGSFQVAFLDALSQMKSEMIMDK"
     gene            7823..8494
                     /locus_tag="VBC28_000932"
     CDS             7823..8494
                     /locus_tag="VBC28_000932"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001904425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEO6814932.1"
                     /translation="MKDTLKLYFVCGTVDCSRKNILTVVEEALQAGITLFQFREKGFT
                     ALQGKEKIAMAKQLQILCKQYQVPFIIDDDIDLVELIDADGLHIGQNDLPVDEARRRL
                     PDKIIGLSVSTMDEYQKSQLSVVDYIGIGPFNPTQSKADAKPAVGNRTTKAVREINQD
                     IPIVAIGGITSDFVHDIIESGADGIAVISAISKANHIVDATRQLRYEVEKALVNRQKH
                     SDVIK"
     gene            8621..9880
                     /locus_tag="VBC28_000933"
     CDS             8621..9880
                     /locus_tag="VBC28_000933"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEO6814933.1"
                     /translation="MRKIIINGGKQLTGEVAVSGAKNSVVALIPATILADDVVVLDGV
                     PAISDVDSLVDIMETMGAKIKRYGETLEIDPCGVKDIPMPYGKINSLRASYYFYGSLL
                     GRYGQATLGLPGGCDLGPRPIDLHLKAFEAMGASVSYEGDSMRLATNGKPLQGANIYM
                     DTVSVGATINTIIAAAKANGRTVIENAAREPEIIDVATLLNNMGAHIRGAGTDVITIE
                     GVKSLHGTRHQVIPDRIEAGTYIAMAAAIGRGIKVTNVLYEHLESFIAKLDEMGVRMT
                     VEEDSIFVEEQERLKAVSIKTSPYPGFATDLQQPLTPLLLTAEGNGSLLDTIYEKRVN
                     HVPELARMGANISTLGGKIVYSGPNQLSGAPVKATDLRAGAALVIAGLMAEGRTEITN
                     IEFILRGYSNIIEKLTSLGADIQLVEE"
     gene            9982..10536
                     /locus_tag="VBC28_000934"
     CDS             9982..10536
                     /locus_tag="VBC28_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO6814934.1"
                     /translation="MDIWTNLGRFAFIETEHVNLRPVAYTDREAFWRIASKRTNLQFI
                     FPVQTSKKESDFLLVHSFMKEPLGVWAIEDKVSHKMFGVIRFENIDLSKKTAEIGYFL
                     KESSWGQGIMTECLKTLSFFAFREFGMDKLIIVTHKENIASQKVALKAHFKQSRSFKG
                     SDRYTRRIRDYIEFQLTRGDYRYE"
     gene            10529..11812
                     /locus_tag="VBC28_000935"
     CDS             10529..11812
                     /locus_tag="VBC28_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEO6814935.1"
                     /translation="MSKHQEILEYLENLAVGKRVSVRSISNHLKVSDGTAYRAIKEAE
                     NRGIVETRPRSGTVRVAQKAKVNIEKLTYAEIARISDSQVVAGIEGLSKEFSKFSIGA
                     MTHRNIEKYLVQGGLLIVGDRDEIQHLALQHQNAILVTGGFNVSPSVCRLADKLQIPV
                     MVTHYDTFTVSTMINHTLSNAKIRTDLKTVEQVYQSQMDYGFLAQDDTVKEFNLLVKQ
                     TKNVRFPIVNQANVVVGVVSVQDILGKDKEVKLATVMSKNIIVAKPRMSLANISQKMI
                     FEDLNMMPVVSDDFELLGVITRRQAVENLSMSQGTDLYTYSDQILSNLQIEDGHFSFL
                     VEPAMIDHTGSLTQGVLTEFLKEICIRVLTRKHQRSIVVKQMTLYFLQPVQIDEIIMV
                     TPTIISEKRREATLDLELKLENKIIAKAMIAVKIN"
     gene            11835..12695
                     /locus_tag="VBC28_000936"
     CDS             11835..12695
                     /locus_tag="VBC28_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HEO6814936.1"
                     /translation="MITLKSAREIEAMDRAGDFLASIHIGLRDIIKPGVDMWEVEEYV
                     RRRCKEENVLPLQIGVDGAVMDYPYATCCGLNDEVAHAFPRHYTLKQGDLLKVDMVLS
                     EPLDKSIVDVSSLNFDNVAQMKKYTETYSGGLADSCWAYAVGEVSQEVKDLMSVTREA
                     MYIGIEKAVIGNRIGDIGAAIQDYAESRGYGVVRDLVGHGVGPTMHEEPMVPNYGTAG
                     RGLRLREGMVLTIEPMINTGTWEIDTDMKTGWAHKTLDGGLSCQYEHQFVITKDGPVI
                     LTSQGEERTY"
     gene            12697..13617
                     /locus_tag="VBC28_000937"
     CDS             12697..13617
                     /locus_tag="VBC28_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEO6814937.1"
                     /translation="MKLKKFFEDLLAKLEYRPIQVFMRHFQSAEMDLSAIAVAYYLLV
                     TAFPLLVIAANIFPYFHINVSDLLSLMQKNLPKNIYEPASRLAVDAFSKPSTGILGFA
                     SLTAFWTMSKSLTSLQKAINKAYGVDQHRDFVISRLVGVGTGLIILFLLTFVLIFSTF
                     SKPVLQIIVNMYDLGDTLTAWLLNLAQPVTFLTIFLGIGILYFILPNARIRKVRYVIP
                     GTLFSTFVIGFFSNLISQYVLNRVEKMVDIKTFGSVVIFILMLWFIFLAHIMILGAIL
                     NASVQEIATGKIESRRGDIMSLIQKSKEEK"
     gene            complement(13634..14089)
                     /locus_tag="VBC28_000938"
     CDS             complement(13634..14089)
                     /locus_tag="VBC28_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEO6814938.1"
                     /translation="MVKLKSLLKKSIQNEVSLYLLFGLLTSLLYLVIRQGIFNFSQDA
                     PFSAIVANIIAILFAFFTNDRFVFKQTKIGQLQRLQTFVIARLGTLGLDLILAVIFVD
                     QFPSIIGQFVQHNLNKINTIESLVSQILIILLNYILSKFVIFKDKKRQL"
     regulatory      14160..14273
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01054"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class II; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF01054"
     gene            14271..14780
                     /locus_tag="VBC28_000939"
     CDS             14271..14780
                     /locus_tag="VBC28_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HEO6814939.1"
                     /translation="MNTFTTRDYAHMAIVTAIYIVLTITPPFNAIAYGAYQFRVSEML
                     NFLAFYHRKYLFAVTLGCMISNLYSFGMIDVFVGGGSTLLFVYLGTILFKQYQKDYLF
                     NGLINKAFFFFSFFFAASMITVAVELKIVAGLPLLLTWLTTAVGELASLLVGAVLVDK
                     LSRHVDFTK"
     gene            14920..16878
                     /gene="ligA"
                     /locus_tag="VBC28_000940"
     CDS             14920..16878
                     /gene="ligA"
                     /locus_tag="VBC28_000940"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001873525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEO6814940.1"
                     /translation="MENRMNELVSLLNQYAKEYYTQDNPTVSDSQYDQLYRELVELEK
                     QHPENILPNSPTHRVGGLVLEGFEKYQHEYPLYSLQDAFSKEELIAFDKRVKAEFPTA
                     AYMAELKIDGLSVSLTYVNGVLQVGATRGDGNIGENITENLKRVHDIPLHLDQSLDIT
                     VRGECYLPKESFEAINIEKRANGEQEFANPRNAAAGTLRQLNTGIVAKRKLATFLYQE
                     ASPTQKETQDDVLKELESYGFSVNHHRLISSSMEKIWDFIQTIEKDRVSLPYDIDGIV
                     IKVNSLAMQEELGFTVKAPRWAIAYKFPAEEKEAEILSVDWTVGRTGVVTPTANLTPV
                     QLAGTTVSRATLHNVDYIAEKDIRIGDTVVVYKAGDIIPAVLNVVMSKRNQQEVMLIP
                     KLCPSCGSELVHFEGEVALRCINPLCPNQIKERLAHFASRDAMNITGFGPSLVEKLFD
                     AHLIADVADIYRLSIENLLTLDGIKEKSATKIYHAIQSSKENSAEKLLFGLGIRHVGS
                     KASRLLLEEFGNLRQLSQASQESIASIDGLGGVIAKSLHTFFEKEEVDKLLEELTSYN
                     VNFNYLGKRVSTDAQLSGLTVVLTGKLEKMTRNEAKEKLQNLGAKVTGSVSKKTDLIV
                     AGSDAGSKLTKAQDLGITIQDEDWLLNL"
     gene            16890..17909
                     /locus_tag="VBC28_000941"
     CDS             16890..17909
                     /locus_tag="VBC28_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEO6814941.1"
                     /translation="MKKQKKARLIYNPTSGQEIMKKNVAEVLDILEGFGYETSAFQTT
                     PTKNSARDEATRAAQAGFDLIVAAGGDGTINEVVNGIAPLKRRPKMAIIPTGTTNDFA
                     RALKIPRGNPIEATKLIGKNQIVKMDIGQAQEDNYFINIAAAGSLTELTYSVPSQLKT
                     TFGYLAYLAKGVELLPRVRKVPVKITHDKGEFIGDASMIFVAITNSVGGFEQIAPDAK
                     LDDGKFTLILVKTANLIEIMHLIRLVLAGGKHINDKRVEYIKTSYLTIEPLSDERMMI
                     NLDGEYGGDAPITLANLKNHIRFFANTDEISDDALVLDKDELAIEAIAQKFANEVDDL
                     EGLGD"
     gene            17913..20213
                     /gene="pulA"
                     /locus_tag="VBC28_000942"
     CDS             17913..20213
                     /gene="pulA"
                     /locus_tag="VBC28_000942"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pullulanase"
                     /protein_id="HEO6814942.1"
                     /translation="MDNTVIVHYHSRKKNYFNLSLWQWRDGSEGQDAHFSRFDSFGAV
                     AILKYPLPYFLSHYYVIVKDKNWHHKTIDYRIDCTQGGARKEVWIVDGDDNLYYSRQA
                     AVSSHHYSRRQPHAYDMAINSRAFDKKWGFDGWLGFEYSETKTAFRLWAPTAERVELI
                     LYHSTDETASVSKVLSMKRGTAVNYKNHKENTHGVWFTELEGNYNYQAYTYRVYYRRR
                     TFKITRDPYSIATTANGKRSIVIAPEALTPEGFKISHGKEAKWRLENPNQAVIYEMHV
                     RDFSISETSGVKTDYHGKFKGLHQKGTVNQHGDKTTFDYVQDLGVNYIQLQPIFDHHQ
                     TFDDDGHYAYNWGYDPENYNVPEASFSSNPHEPATRILELKSAIQAYHDAGIGVIMDV
                     VYNHTFSSTDSAFQLTVPDYYYRMNHNGTFQNGSGCGNETASEKEMCRKYILDSVLYW
                     VKEYNIDGFRFDLMGLHDVETMNIIRNELNKIDPRILVYGEGWDMGAGLTPQNKAKKD
                     NAYQMPGIGFFNDDVRDAVKGAEIYGEFKKGLVSGNSTEDIVAKGILGSDELVSYIDP
                     SQVLNYVEAHDNYNLNDLLWELHPNDNEKQHIYRVEVASAMNLLMQGMAFMQLGQEFL
                     RTKCYPTGDKGQLTQADKERAMNSYNAPDQVNQVNWDNVTFHKSTINFIRKIITLKTN
                     SPYFSYSSFEEIRKHVFVESAQYHSGFISFTVEEHKKIKIIFNIKGKRLQNSFIGDII
                     ISNHEEGKAIDSLDDELSVMILDITK"
     gene            20419..22287
                     /gene="glgB"
                     /locus_tag="VBC28_000943"
     CDS             20419..22287
                     /gene="glgB"
                     /locus_tag="VBC28_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HEO6814943.1"
                     /translation="MSNKDELYTFGIGENFHLQNYLGVHSENGSFCFRVWAPNAENVQ
                     VIGDFTDWRNRPLQMNKNQAGVWEANSLDAREGDLYKYLVTRKGGQVVEKIDPMAVYM
                     ERRPGTASVIKVLRNKKWEDGLWMGRRKRLGFQKRPINIYEVHAGSWKKDDFGHPMTF
                     SQLKDYLIPYLVEMNYTHVEFMPLMAHPLDMSWGYQLMGYFAFEHTYGTPEEFQDFVE
                     ACHKNNIGVLVDWVPGHFIQNDDALAYFDGTATYEYQNHDRAHNYRWGALNFDLGKNQ
                     VQSFLISSALFWIEHYHIDGIRVDAVSNMLYLDYDEGPWEANQFGDNRNLEGYHFLRK
                     LNKVIKERHPNVMMIAEESTASTPITKDLESGGLGFDFKWNMGWMNDILRFYEEDPLY
                     RQYDFNLVTFSFMYIFNENFVLAFSHDEVVHGKKSMMHKMWGDRYNQFAGLRNLYAYQ
                     MCHPGKKLLFMGSEFGQFLEWKYNDQLEWENLNDDMNQKMQRYTKQLNQFYKDHKCLW
                     RIDDSFDGLEIIDADNKSETVLSFIRKDDKGDLLLCVFNMTPVERPNFTIGVPQAGIY
                     EEVLNTEMEEFGGVWKNHNPVTKTQVATWKDYDHTLSFTLPALGASVWRIKRRLRK"
     gene            22329..23468
                     /locus_tag="VBC28_000944"
     CDS             22329..23468
                     /locus_tag="VBC28_000944"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019785022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HEO6814944.1"
                     /translation="MKNEMLALILAGGQGTRLGKLTQSIAKPAVQFGGRYRIIDFALS
                     NCANSGINNVGVITQYQPLELNTHIGNGSSWGLDGIDSGVTVLQPYSATEGNRWFQGT
                     SHAIYQNIDYIDRINPEYVLILSGDHIYKMNYDDMLQTHKDNLASLTVAVLDVPLKEA
                     SRFGIMNTDSNDRIVEFEEKPEHPKSTKASMGIYIFDWKRLRTVLIDGEKNGIDMSDF
                     GKNVIPAYLESGERVYTYNFDGYWKDVGTIESLWEANMEYIGEDNKLHSRDRSWKIYS
                     KNLIAPPNFMTEDANVKDSLVVDGCFVAGNVEHSISSTNVQVKPNAIIKDSFVMSGAT
                     IGEGAKINRAIIGEDAVIGDGVVIDGSKEVEVIGYKEVAGVPNED"
     gene            23458..>23622
                     /locus_tag="VBC28_000945"
     CDS             23458..>23622
                     /locus_tag="VBC28_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000687083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HEO6814945.1"
                     /translation="MKIDKYTAILGNTVSYPDMHSLTDNRPLASLPFDGKYRLIDFQL
                     SSLANAGVRSI"
BASE COUNT         7726 a         3540 c         4805 g         7551 t
ORIGIN      
        1 aaaaatatct taattcaact gaaccgtata gtattgaaac taacttacct tcaaattata
       61 aagaattgaa ggaaaagcaa ataaaaaaat tggaatatgg atacaaaaag tcttctaaaa
      121 tttatacgtc tgctcattat gcactatata gtgaagaaat tgatgctgca aaagaattgc
      181 ttcaaaaagt gaagattgca aaagataatt ataatgaaat taagtccatg aatctaagcc
      241 cttcaatttt taatcagtat ttacaacttc ttcaaatagt tataagttca gagataaatc
      301 taaaaaaggc actcgataac actgtggatt tacccataga aaataatttt aatacattgg
      361 acattcaata taataaatta gacactgcta ttaagtcact tcgaaaattt gtaactaagt
      421 ataaacaaga agtaagaaaa gctactaaga gttattctaa aaaagaatta gtaaatgcag
      481 agttaacaaa agtaatttct aatgacaata ttttattaga catgcaagca attagttcca
      541 attatgggtc tactaaaaag tttgtttatt cagtcaaaag attaccttac gtcccgcaag
      601 taattatgac aacaacgtct aatgtactta tgcctcaaaa gcaagttgaa aaagtgaagc
      661 tactcacccc atttacaatt tctaataaag aagtattgaa tcatgactca ctagtagaaa
      721 atgatgctca aaaacaaaaa gtagaacagg aaaaaactaa atcgctagct cctcaaaaag
      781 gggctgtaaa agaacaaact gagcaaaaag tatctggaaa tactcaagag atagaaaaga
      841 aatctgaaac tgtggcaact ccacaacaaa gttcagttgc gcaaacttct gtccaacagc
      901 cggctccggt tcaatcagtt gttcaagaat ccaaagcttc tcaagaggag attaatgcag
      961 cacacgatgc tatttcggcg tataaatcaa cagtcaatat tgctaataca gccggtgtaa
     1021 caactgcgga aatgaccacg ctcattaata ctcaaacttc taatctttct gatgttgaga
     1081 aagctttagg aaataataag gttaataatg gtgcagtcaa tgtattgaga gaagatacag
     1141 ctcgtcttga gaatatgatt tggaatcgtg cttaccaagc tattgaagaa ttcaacgtcg
     1201 ctcgtaatac ttataataac caaatcaaga cagaaacagt tccagttgat aatgatattg
     1261 aagctatttt agcaggttct caagctaaaa ttagccattt ggacaatcgt atcggagcgc
     1321 gccacatgga tcaagctttt gtagctagtt tattagaagt tactgagatg agtaaatcaa
     1381 tctcatcgcg tataaaagag tagacactgc tatcaaggcg atcttaaact tttgtattaa
     1441 actaacctaa aagatagaaa gagactaata tgaaaaaaat aacaacttta atcttagcta
     1501 gtagcttatt actagttgca acgacatcgg ttaaagctga tgataacttt gaaatgccaa
     1561 cgcgttatgt taaaatgagt gaaaaatcaa aagcatttta tcaaagacta caagaaaaac
     1621 aacgtaaggc acatactact gtgaagactt ttaataattc agaaataagg catcaactac
     1681 ctcttaaaca agaaaaggct agaaatgata tctacaattt aggcattctt atttctcagg
     1741 agtctaaagg gttcatccaa cgtattgata atgcctattc tttggaaaat gtctcagata
     1801 ttgttaatga agctcaggct ttgtataaac gtaactatga tttatttgaa aaaatcaaat
     1861 ctacacgtga taaggttcaa gtcttacttg catcgcatca agataataca gacttaaaaa
     1921 acttttatgc tgagttggac gatatgtatg aacatgttta tctcaatgaa agtagagtgg
     1981 aggcgataaa cagaaatatc caaaaatata attagtttct aaactaacaa acattcctaa
     2041 atataagaca taaaacccta ctcattgatt agtgagtagg gttttactgt tttaaatagc
     2101 tttctgatca gaatgtaagc cttgtcattt caaaggaact atgttattat tcttaagtaa
     2161 attaaatagg acatttgggg tgcgtaacag ctgagattat acccattgaa cttgatgcag
     2221 ttagtactgt cgaagggaaa tgtgtgccat tacaagtttt ttgtggtgga aaagggcgta
     2281 gtgtcttctt agtagacagc tagctctagt taatcagata ataaactccg tttgcttgca
     2341 gatggagttt tttgttgtca aaattttttg taggttgttg ccccttatct gtcatagaga
     2401 aggagttatg atgactaatc ataaaacaaa aaaattaacg gttttagcaa ttatgattgc
     2461 tttagatgtt tttttaacgc ccatttttag aatagaaggc atggccttta tgtctagtgt
     2521 ggttaatatt atcgctggag ttataatggg accaatctat gcattagtta tggcacttgt
     2581 aacggctttt attcgtattt taactcaagc aggagtagca tctgttgcac ctttagctat
     2641 tctaggagct ggtccaggag ctttcttagc gggttggttt tatcggtttt atgcgaaaga
     2701 ctggatgtct tgggtgggtg agtttatagg aacagggata attggagcac ttttatcagc
     2761 tccaattatg gtctggtttt ggacttggac agcggatgga aatcacgaag tgttagctaa
     2821 agcatctgca gctcaatcca tttttctttt tatgccaagg tttatagggg cgactataat
     2881 tggcggtgtt attgctttgt tgctacttca gggattaaag cgggttcctg tatttatgac
     2941 aatacaagca ttatttaaag gagaaaaagt aaaatgaaat tgagtttaaa agatgtatta
     3001 ttgattgctc tattagcggt tgttctgggc gttgtttact ttggagcagg ctatatttca
     3061 aatgcttttg taccttttgt aggccctata gcgcatgaag ttatttatgg tatttggttt
     3121 gtggcaggtc caatggctct ttatatattg aggaaaccag gaaccgctat tgtagcagaa
     3181 ttgttagctg ctttgataga ggttttaatt ggatcaattt atggaccaag tgtactcgtt
     3241 attggtaccc ttcaaggttt agggtcagaa ttaggcttta cactatttag gtatcataat
     3301 tacaagttgc cagcttttat attgtctgct attttgacat caatttttag ttttgcatgg
     3361 agtttttatg ccaatggact tagtgcgttt tcattttcat ataacattct gatgcttata
     3421 gtaagaactg tttcatctat tattttcttc ttattaacaa agaatatatg cgaccaattg
     3481 catagaagtg gtgtactgaa tgcttatggg attcatgaaa ggaagtagcc tatgctatct
     3541 gttgaaaaat tagcttgcac ccatggcgat agtcattatt tatttgatga agtgactttt
     3601 agtcttaacc caggagaacg aattttaatc agtggctatt cagggtgtgg taagagtaca
     3661 ttggctcttt tactatcagg gttaaaagaa agtgggaaag gtcaagtttt attaaacggc
     3721 tcattaatag aaccaagcga tgtggggttt ctttttcaaa atcctgattt gcaattttgt
     3781 atggatactg tggcgcatga gttgtatttt attttagaaa atttacaaat agaaccggag
     3841 caaatgcaag atagaagtga gtttgtattg gcacaagttg gtttaaaagg atttcagaat
     3901 cggttgattt atactttatc tcaaggtgaa aaacagcgat tagcattggc gaccattttt
     3961 ttaaaatctc ctaaacttat tattttagat gaagcttttg cgaatttgga tcaagagagt
     4021 gccagtcaac tattacaatt agttcttaat tatcaagcaa acaatcaatc aatgctgatt
     4081 gtaatcgatc atttgattac ttattatcaa gatataatgg atcactattt ttggttggaa
     4141 aaacggttga caagggtcaa ttttgattat atgctcaatc gtttgaatgt cttcgaatta
     4201 gaaaagaaaa gtcataatac aggtgacaaa ctgttatcaa ttaaagattt ccaagttaaa
     4261 ctttcaaaaa acaaattcat ctcttacctt gattttgatt tagcaagtgg ggagaggttg
     4321 tgcttagatg gaccaagtgg cgtggggaag tctagtttgt ttatgggatt gttagggctt
     4381 tataggacta aagggaaaaa acaatttact catagaaagc aaattcctat aagcttttta
     4441 tttcaaaatc ccttagacca atttattttt tcaacagttt atgacgaaat ttttcaggtt
     4501 tgcaaggata gtaacaaagc gagggatatt ttggaaacca taaatctttg ggataagaaa
     4561 cagttttcac catttcaact aagtcaaggc caacagaggc gattagccat tggcagtatt
     4621 ttagctagtg atagtaaact tctattatta gatgaaccaa cttatggaca agatgcctat
     4681 cacgctaata tgattacaac tcttcttttg agttattgtc acaaaaatca ttgtggggtt
     4741 atttttacta gccatgatcc tcacttaaaa gagtgttttg caacacgttt cttggaggtg
     4801 tcatcttgtt caatttgaaa aactatcatc cagctattac cttcctatgt ttattaatac
     4861 caaccttttc gtttagtttc acattgaggt taagaacgtc tttgttattt ctaattatag
     4921 tagtaacact gcaatgcttc gtaaaggttt ctttaaaaac ttgggcgaag gtcaatctga
     4981 tatcatttgt gatgggactc tctttatttc tagggacgta tttttgggga aaattacccc
     5041 atcaattcgt cttagctagt ctagtggcct gtcgtccgct tatttttatg aacgtgggtt
     5101 tgttatttca tgcatcacat tctaattatg attttataga atctctttat caaactttca
     5161 aagtgcctag tcattttgca tatggtattt ttgcggtctt taatcttcta ccattaatta
     5221 aattacaata ccaacgtaat cgactcgcat tccgactgaa gaatcaggta acttgggcat
     5281 tatcgcctag actaatattg tcagttttat taaagacgat atattgggta gaacagttag
     5341 agttagcaat gctttctaaa ggttttgaag tgggtaaaga gcgaacacat gcttcaactt
     5401 atcccgttcg ttttcgagat tatagtttac ttggtatgag tattttattg tctataggaa
     5461 tgatttttaa ataagtacaa ttagtttagt aatacaattg tgcatttata tagaatgagc
     5521 gtgttctgct catgagatca taacaaagga gattactatg ttaaaagata agatgattgc
     5581 taaaggagca aaatatatcc aatccatcta tcaagatcca tttattcaag gaattataaa
     5641 agggcgttta gaccatgacg taatctgcca ttatttacag gcagataata tctaccttgg
     5701 gaaatttgct gatatttatg ccctgtgctt ggcaaaatct gataatttac gagataaaca
     5761 atttttcctt gaacaaattg actttacatt aaatcgggag ttagcagatg gtgaaggccc
     5821 acatcaagcc ttggccgctt atactaatcg atcttatcag gatattatcg aaaagggtgt
     5881 atggtacccg tcagcagatc attacattaa acatatgtat tttcattttt acgaaaatgg
     5941 tattgctggt gctttagctg caatgagccc atgtccatgg atttaccatc aattggctaa
     6001 aaaaataatt gaggagaacc agtttttgaa tggtaaccct tttaataatt ggataacttt
     6061 ttatgctaac gatacagttg aagagttaat ggaaaactat tttcgtatga tggattatta
     6121 tgcgcagaat ttgtcaaaag agaagcaagc tgatttggta gatgcttttg tgaaatcttg
     6181 tcagcatgag agacgttttt ttcaaatggc aataaatcaa gaaaaatggg aggattaata
     6241 tgacttattt agcaccagtt ttaactattg caggaactga tcctagcggt ggagcaggga
     6301 ttatggcgga tttaaagaca tttcaagctc gtcggactta cgggatggct gttgtgacta
     6361 gtgttgttgc tcagaacact tgtggggtta gaggcgttca gcatatagaa acagctatca
     6421 ttgatcaaca actagcttgt gtctatgatg atattaagcc aaaggcggtt aaaacaggaa
     6481 tgttagcgga gagggaaaca attagtttag tcgcttcata tttaaaaaaa tatccgcaac
     6541 cttatgtatt agatcctgtt atggtggcaa ctagcggaca tcgtttaatt gattcagatg
     6601 ccgtagaagc attaaaagaa gacttattgc cattagcaac tattatcaca ccaaatttac
     6661 cagaagctga ggttttagtg ggatatgact tatcagatga agtaagtatt attaaagcag
     6721 gttacgatat tcaaaaacaa tattctgtta gaaatgttct tataaaggga ggacatctag
     6781 atggcttggc taaagattat ttatttttgg aaaaagaagg gttgataact cttagtaatc
     6841 aacgtatcaa tactattcat actcatggga ctgggtgtac atttgcagca gtcgttgcag
     6901 cggagttagc taaagggcaa tcaattctaa acgcggtaag taccgctaaa tcatttatta
     6961 cttcagctat cgaaacagcg cctgagttgg gccttggaaa cggtcctgtc aatcatacta
     7021 gctatcaagg agactaaaat gtatttagaa caattaaaag aggtaaatcc tttaaccatt
     7081 tgtatcacta ataatgtggt caaaaacttt acagccaatg gtttattagc tttaggagct
     7141 tctccggcta tgagcgagtg catagaggac ttagaagact tgttaaaagt tgcagatgca
     7201 ttattaatta atataggaac actaacaaaa gaaagttggc aactttacca agaagcaatc
     7261 aaaatagcaa ataaaaacca agttccagtt gtattagatc ctgtagcggc aggagcaagt
     7321 cgttttcgat tagaagtgtc acttgattta ttgaagaatt attctattag tttacttaga
     7381 ggaaatggta gtgaaatcgc tgctttgatt ggtgaaaaac aagcgtctaa aggggcagat
     7441 ggcggaaaag tggctgattt agaatcaatt gctgtaaaag ccaatcaagt ttttgatgtg
     7501 ccagtggttg tcacaggaga aactgatgct atcgctgtta gaggagaagt tcgcttatta
     7561 caaaatggta gtcctttaat gccacttgtt acaggaactg gatgtctatt aggggcagta
     7621 ttggctgctt ttattggtag ttctgacaga agcgatgatc ttgcttgtct gacagaggcc
     7681 atgactgttt ataatgttgc tggagaaatt gcagagaagg tggcaaaagg taagggggtg
     7741 ggaagtttcc aagttgcatt tttagatgcc ctttcccaga tgaaatcaga aatgattatg
     7801 gacaaatagg tggtgtcagt agatgaaaga tacattgaaa ttatattttg tttgtggaac
     7861 tgtggattgc tctaggaaga atatattgac tgttgttgaa gaagccttac aagcaggtat
     7921 aactctattt caatttagag aaaaaggttt tacagcatta caaggtaagg aaaaaattgc
     7981 tatggctaag caactccaaa tactttgtaa gcaatatcaa gttcctttta ttattgatga
     8041 tgacattgac ttagttgagt taattgatgc tgatggtctt catattggac aaaatgattt
     8101 acctgttgat gaggcacgga gacgtttacc agataaaatc atcggtttat ctgtgagtac
     8161 aatggatgaa tatcagaaat cacaattatc tgttgttgat tacatcggaa ttggtccttt
     8221 taatccgaca caatcaaaag cagatgctaa gcctgctgtt ggcaatagga caacaaaggc
     8281 agttagagaa attaatcaag atattccaat cgttgcaata ggcggaatca ctagtgattt
     8341 tgttcatgat attatagaat caggtgcaga tgggattgct gttatatcgg ctatttcaaa
     8401 agcaaatcat attgtagatg caaccagaca attgcgttat gaagttgaaa aagctctggt
     8461 taataggcaa aaacacagtg atgtaatcaa ataaggctac atcgttaatt ataagaattt
     8521 ttaagatatt atatcatcac acaatactgg tatttgaaaa agaatttaaa ctatgataat
     8581 atagtagagt tgaattctag ttaaagaaag atttgaaagt atgcgtaaaa ttattattaa
     8641 cggtggcaag caactgacgg gagaagttgc tgtatcgggt gccaaaaata gtgtagttgc
     8701 cttgattcct gccactattc ttgctgatga tgttgtggtc ttagatggag ttccggctat
     8761 ttctgatgtt gacagtttag ttgacattat ggaaactatg ggagctaaga ttaagcgtta
     8821 tggtgagacg cttgaaattg atccatgtgg cgttaaagat attccaatgc cttatggcaa
     8881 aatcaatagt cttcgtgcat cctattattt ctatggaagc ttattggggc gttatggtca
     8941 agcaacatta ggtttacctg gtggttgtga tttaggcccc cgtccgattg atttacattt
     9001 aaaagctttt gaagcgatgg gagctagtgt ttcttatgaa ggtgattcaa tgcgtctagc
     9061 aacaaacgga aagcctttgc aaggagcaaa tatttatatg gatactgtca gtgttggtgc
     9121 gacaattaac actataatcg cagcagctaa ggcaaatggc cgtacggtta ttgaaaatgc
     9181 tgcacgtgaa ccagaaatta ttgacgtggc tactctttta aataatatgg gagctcatat
     9241 tcgtggtgct gggactgatg ttattacaat tgaaggtgtg aaatcgctcc atggcacgcg
     9301 tcatcaagta attccagatc gtattgaagc aggaacttat attgctatgg cagcagctat
     9361 cggacgagga ataaaagtga ccaatgtgtt atatgaacac cttgagagtt tcattgctaa
     9421 gttagatgag atgggagttc gaatgacagt tgaagaggat tcaatttttg tcgaagaaca
     9481 agaaaggtta aaagctgtat ccataaaaac ctcaccttac ccaggatttg ctacagattt
     9541 acaacaacct ctaactcctt tattactaac agcagaagga aacgggagtt tattagatac
     9601 tatctatgag aaacgtgtta atcatgttcc cgaattagca cgtatgggag ctaatatttc
     9661 taccctaggg ggcaaaatag tttatagtgg tcctaatcaa ttatctggtg cacctgtaaa
     9721 agcgacagat ttgcgtgcag gcgctgcttt agttatcgct ggcttaatgg cagagggacg
     9781 aactgaaatt acgaatatcg agtttatttt acgtggatat tctaatatca tcgagaaact
     9841 aacatcattg ggtgcggata tccaattggt tgaggaatag tatatataac aaagagttga
     9901 gaatatttct tagctctttt cttcttgttt tagattaagt cacttatagg tatttgaaat
     9961 ctcttgtggt ataatattat catggatata tggacaaatt taggacggtt tgcattcatt
    10021 gaaacagaac atgttaacct acgtcctgtt gcctacactg atagggaagc tttttggcga
    10081 atagcttcaa aaaggacaaa tttacaattt atttttcctg ttcaaaccag taagaaggaa
    10141 agtgattttc ttttagttca ttcttttatg aaagagccat tgggagtttg ggcgattgaa
    10201 gataaagtta gtcataaaat gtttggagtt atccgttttg aaaacattga tttatctaaa
    10261 aagacagctg aaattggcta ttttttgaaa gaaagttctt ggggtcaagg cattatgact
    10321 gaatgtctaa agacactttc tttttttgct tttcgagagt ttgggatgga caaattgatc
    10381 atagtaacac ataaagagaa tattgctagt cagaaggttg ctttgaaggc gcatttcaaa
    10441 caatctagga gttttaaagg aagcgaccgt tacacacgcc gtataagaga ctatattgag
    10501 tttcaattaa ctaggggtga ttatcgttat gagtaaacat caagaaattt tggagtacct
    10561 agaaaattta gctgttggta agagggttag tgtacgcagt atttcaaatc atttaaaggt
    10621 gagtgatggc actgcatacc gcgctataaa agaggcagaa aataggggta ttgttgagac
    10681 tagaccgcgt agtgggacag tacgtgttgc tcaaaaagca aaggttaata ttgaaaaact
    10741 gacttatgct gaaattgcaa ggattagtga ctcacaagtt gttgcaggaa tagagggact
    10801 cagcaaggag tttagtaaat tttctatagg tgcaatgact cacagaaata ttgaaaaata
    10861 tttggtgcaa ggagggttat tgattgttgg cgaccgtgat gagatacaac atttagcctt
    10921 acaacatcaa aacgctatct tagttacagg tggctttaat gtttccccat ctgtttgtcg
    10981 tttagcagat aaacttcaaa ttcccgtaat ggtaacacac tatgatacat tcacagtatc
    11041 aacgatgatt aatcacactt tgtcaaatgc caaaattcgt actgatttaa agaccgttga
    11101 acaagtctat cagtcgcaaa tggattacgg tttcttagcg caggatgata ccgtaaaaga
    11161 atttaatctt cttgttaaac aaactaaaaa tgttcgtttt ccaatagtca accaagctaa
    11221 tgttgtcgtt ggtgttgtta gtgttcaaga tattcttgga aaagataaag aagtaaaatt
    11281 ggcaacagtc atgtcaaaaa atatcattgt tgctaaacct cgaatgagtc tagctaatat
    11341 cagtcaaaaa atgatttttg aagatttgaa tatgatgcca gtagtatcgg atgactttga
    11401 attattaggc gttattacaa gacgacaagc tgtagaaaat ttatcgatgt cgcaagggac
    11461 ggatttatac acctatagtg atcaaatctt gtctaattta caaattgaag atggtcactt
    11521 ttcgttttta gtagaacccg ctatgattga ccatacaggt agcttaacac aaggagtttt
    11581 gacggagttc ttaaaagaaa tttgtattcg tgtcttaact cgtaagcatc aacgcagtat
    11641 tgttgttaaa caaatgacac tttatttctt gcaacctgtt caaattgatg aaataataat
    11701 ggtaacgcca acaattattt cagaaaaacg gcgcgaagct acactcgatt tagaattaaa
    11761 attagaaaat aaaattattg ctaaagctat gatagcggta aaaattaatt agatataagg
    11821 agaataaata gaaaatgatt acattaaagt cagcacgcga aattgaagca atggatagag
    11881 caggtgattt tttagccagc atccacattg gacttcgtga tattatcaaa ccaggagttg
    11941 atatgtggga agttgaagaa tacgttcgta gacgttgtaa agaagaaaac gtattgccat
    12001 tgcaaatcgg ggttgatggt gcagttatgg attacccata tgctacttgt tgtgggctta
    12061 atgatgaagt ggctcatgcc tttcctcgtc attataccct aaaacaaggt gaccttttaa
    12121 aagtagatat ggttttaagc gaacctttag ataaatcaat tgttgacgtt tcatcgttaa
    12181 actttgacaa cgtggctcaa atgaaaaagt acacggaaac atatagtggt ggtttggcag
    12241 actcgtgttg ggcttatgct gtaggtgaag tttcacaaga ggtgaaagac ttaatgtctg
    12301 tgacaagaga agcaatgtat attgggattg aaaaagcagt tatcggaaac cgcattggtg
    12361 atattggagc tgctattcag gattatgcag agagtcgtgg ttatggagtt gttcgtgatt
    12421 tggttggtca cggtgttggt ccaacaatgc atgaagagcc tatggtgcct aactatggta
    12481 cagcaggtcg aggtctccgt cttcgtgagg ggatggtttt aaccatcgaa ccaatgatta
    12541 atactggtac ttgggaaatt gatacagaca tgaaaactgg ttgggcacat aaaaccttag
    12601 atggtggtct ttcttgtcaa tatgaacatc aatttgttat cactaaagat ggtcctgtta
    12661 ttttaacgag tcaaggagaa gaaagaactt actaaaatga aattaaaaaa attctttgag
    12721 gatttgctgg ctaaactaga atatagaccc attcaagttt ttatgcgtca ttttcaaagt
    12781 gcagagatgg acttatcagc catagcagtt gcttattatc tattagtgac agcttttcca
    12841 ttactagtta ttgctgctaa tatttttcct tattttcata ttaatgtgtc ggatttattg
    12901 tcacttatgc aaaaaaatct tccgaaaaat atttatgaac ctgcctcacg cttagcagta
    12961 gatgcttttt cgaaaccgtc taccggcatt ttgggattcg cgtcattaac agctttttgg
    13021 acaatgtcta aaagtttgac ctctcttcaa aaagccatta ataaagcata cggtgttgac
    13081 caacacagag actttgtgat tagtcgttta gtgggtgtgg ggactgggct gatcatccta
    13141 tttttactga cttttgtttt aattttttca accttttcaa aaccagtttt acagataatt
    13201 gtaaacatgt atgatttggg agatactcta acggcgtggc ttttaaatct tgcacaacca
    13261 gttacatttt tgactatatt tttaggtatt ggaattttat actttatttt acctaacgca
    13321 cgaattagaa aagtacgtta tgtcattcca gggacattat tttcaacgtt tgttattggt
    13381 ttttttagta atttaattag ccaatacgtc ttaaataggg ttgagaaaat ggtagatatt
    13441 aaaacgtttg gttcggtcgt tatctttatc ttaatgctat ggtttatttt cttagcccac
    13501 attatgattt taggtgcaat actgaatgcc agcgttcaag aaattgcgac aggaaaaatt
    13561 gaatcaaggc gtggagatat tatgtctctt atccaaaaat ccaaagaaga aaaataagaa
    13621 aagacagctc ttgttagagt tgtctttttt tgtctttaaa aataacaaat ttgctcaaga
    13681 tgtagttgag taaaataatt aatatttgag atacaaggct ttcaatggtg ttgattttat
    13741 ttaaattgtg ctgaacgaat tgtccgataa tagaaggaaa ttgatcaaca aaaataacag
    13801 ctaatatcaa atctaatcct aatgtgccta atctagcgat gacaaatgtt tgtaagcgtt
    13861 gaagctgtcc aattttagtt tgtttaaaaa caaatcgatc atttgtaaaa aaagcaaaaa
    13921 gtatagctat tatgttagca actattgcag agaatggagc gtcctgcgag aaattaaaaa
    13981 tcccttgtcg aataactaaa taaagaaggg atgttaatag tccgaacagt aaatataagc
    14041 tgacttcatt ttgaatactt tttttcagta acgattttaa tttaaccata tttttattat
    14101 atcacggatg cttcaaagtg atgaggaaat ctcccataag cttggcttta ctttttgttt
    14161 gtgatatact tagtaagttg ttttacacaa cccttgatgc ttagttccct ttcatcaagc
    14221 atattacaaa cgttagaagt gttaactatc taaacgttaa aggagaaatt atgaacacat
    14281 ttacaactcg cgattacgct cacatggcta ttgtaacagc tatctatatc gttttaacca
    14341 taacgccacc ttttaatgct attgcttatg gtgcttatca attccgtgtt tcagaaatgc
    14401 taaatttctt ggccttttac caccgaaaat acctttttgc ggtaacgtta ggatgtatga
    14461 tttctaatct ttatagtttt ggaatgattg atgtctttgt aggtggtggt tcaaccttac
    14521 tttttgttta tcttggaacc attttattca agcaatatca aaaagattat ctatttaatg
    14581 gccttattaa caaagcattc tttttctttt ctttcttctt tgctgcttca atgattacgg
    14641 tagcagttga attgaaaatt gtagcaggat tacctttatt attaacatgg ttaacgacag
    14701 ctgttggtga attggcttca cttttggttg gagcagtcct tgtggataag ttgagtagac
    14761 acgtcgattt tacaaaataa atatattata aagcgcgaga cattgatctc ggcttttttg
    14821 tttgggttta tagttagcat agctagaaaa tgctgcaact tttatgagaa atgctccaat
    14881 ttttactaat atccttagtc tttgtgataa aatagaaata tggaaaatcg tatgaatgaa
    14941 ttagtatctt tgttgaatca gtacgcaaaa gagtattata cacaagataa tcccacagtt
    15001 tctgacagtc aatatgacca gttatatcga gagttagtag aattagaaaa gcaacatcca
    15061 gaaaacattt tgccaaatag tcccacacat cgagtgggtg gtttagtttt agaaggcttt
    15121 gaaaaatacc aacatgaata tcctttgtat tcattgcagg atgctttctc aaaagaagaa
    15181 ttgatagcct ttgataaacg tgttaaagca gagtttccaa ctgctgctta tatggcagag
    15241 ctgaaaattg atggtctatc tgtctcgttg acttatgtaa atggtgtttt acaggttggg
    15301 gcaacacgtg gtgatggcaa tattggagaa aatattactg aaaatttaaa gcgtgttcat
    15361 gatattcctc tacatttaga ccaaagttta gatattactg ttcgcggaga gtgctacctt
    15421 cccaaagaat cttttgaagc tattaacata gaaaaacgag caaatggtga gcaggaattt
    15481 gctaatccta ggaatgcagc agcaggaacc ttgcgccaat taaacacagg aattgttgcg
    15541 aaacgtaaat tagcaacttt tctttatcaa gaagctagcc ctacgcaaaa agaaacacaa
    15601 gatgatgttt taaaagagct agaaagctat ggcttttcag tcaatcatca tcgtttaatt
    15661 agctcgtcta tggagaaaat ttgggatttt atccaaacta tagaaaaaga tagagtttcc
    15721 ctcccttatg atatcgatgg tattgttatt aaggttaata gcttagcaat gcaagaagag
    15781 ctgggcttca ctgtaaaggc acctcgttgg gcgattgcat ataaatttcc tgctgaagaa
    15841 aaggaagcag aaatattatc tgttgattgg acagttgggc gaacaggagt cgtaacacca
    15901 acagctaacc taactccagt tcaactagca gggacaaccg ttagtagggc cactctgcat
    15961 aatgtagatt atattgccga aaaagatatc cgtattggag atactgttgt tgtatacaaa
    16021 gcaggggata tcatccctgc agtcttaaat gtcgttatga gtaagcgaaa ccagcaagaa
    16081 gtcatgctta ttcctaaact atgtccttct tgtggtagtg aattagttca ttttgaaggt
    16141 gaggtggctt tacgttgtat taatccgctg tgccccaacc aaatcaaaga acgtttagct
    16201 cattttgcta gccgagatgc tatgaatatt acaggttttg gtccatcctt agtcgaaaaa
    16261 ttatttgatg ctcatttaat agcagatgtt gcagatattt atcgtttaag tatagagaat
    16321 ttattaactt tagatggtat taaagagaaa tctgcaacta agatttatca tgcaatccaa
    16381 tcttcaaaag aaaattctgc tgaaaaactt ctttttggcc taggtattcg tcatgtggga
    16441 agcaaggcga gccgcctttt attggaggaa tttggtaatt tgaggcaact aagtcaagca
    16501 agtcaagaaa gtattgcctc aattgatgga ctaggagggg ttattgccaa atcacttcat
    16561 accttctttg aaaaagaaga ggtagataaa ctactagagg aattaacttc ctataacgtt
    16621 aattttaatt atctaggaaa acgagttagt acagatgcac aactttcagg tttaacagtc
    16681 gttttaaccg gtaagcttga aaaaatgact cgtaatgaag caaaagagaa attacaaaac
    16741 cttggtgcta aggtgacagg tagcgtttct aaaaagactg atttaattgt tgcaggaagc
    16801 gatgcaggaa gtaaactaac caaggctcaa gatttaggga ttacaataca agatgaagac
    16861 tggttactca acctttaagt gaggaagaaa tgaagaaaca aaaaaaagcg cgtttaattt
    16921 ataacccaac ttctggtcaa gaaattatga aaaaaaatgt agcggaagtt cttgatatac
    16981 ttgaaggatt tgggtatgaa acatcagcct ttcaaacaac accaactaaa aattctgcca
    17041 gagatgaagc tactagagct gctcaagctg gttttgattt gattgttgca gcaggtggag
    17101 atggtactat taatgaagtt gttaatggta ttgcaccttt gaaacgtcgt cctaagatgg
    17161 caattattcc aactggaaca actaatgatt ttgctcgtgc cttaaaaata ccgcgaggta
    17221 atcctattga agcaactaaa ttaattggta aaaatcaaat cgttaagatg gacattggtc
    17281 aagctcaaga ggacaattat tttattaata ttgcagctgc aggtagtttg acagagttaa
    17341 cttatagtgt accaagtcaa ttgaaaacaa cgtttggcta tcttgcttat ctagcaaagg
    17401 gtgttgaatt attacctcga gttcgaaaag taccagtcaa aattactcac gataagggtg
    17461 aatttatcgg tgatgcttca atgatttttg ttgccatcac aaattctgta ggagggtttg
    17521 aacaaattgc acctgatgcc aaattggatg atgggaaatt tacccttatt ttagtaaaaa
    17581 ctgcaaatct tattgagatt atgcatctca ttcgtttggt gctagcaggt ggaaaacata
    17641 ttaatgataa acgagtagag tatattaaaa cgtcttatct aactatcgaa cccctatcag
    17701 acgagcgtat gatgattaac ttagatggag aatatggagg agatgctccc attactctag
    17761 caaaccttaa aaatcatatt aggttctttg ctaatactga tgaaatttca gatgatgctt
    17821 tagttttaga taaagatgaa ctagcgatag aagctattgc acagaagttt gctaatgagg
    17881 tagatgattt agaaggatta ggagattgat gaatggataa tactgtcatt gtacattacc
    17941 atagccgaaa gaaaaattac tttaatttaa gtttatggca gtggcgcgat ggctcagagg
    18001 gtcaagatgc tcatttttca cggtttgaca gtttcggtgc tgttgcgata ctaaaatatc
    18061 ccttgccata ttttttaagc cattattatg tgattgttaa ggataaaaat tggcatcata
    18121 aaacaataga ttatcgcatt gattgtactc aaggtggtgc acgtaaagaa gtttggattg
    18181 tagatgggga tgacaatctg tattattctc gtcaagcagc agttagtagc catcattata
    18241 gtagacgcca acctcatgcc tacgacatgg caatcaatag tagagcgttt gacaaaaaat
    18301 ggggatttga tggttggtta ggttttgaat attcagaaac taaaacggct tttagattat
    18361 gggcccctac tgcggaacgt gtagaactga ttttatatca ttcgacagat gaaacagcaa
    18421 gtgtatcaaa agttctttct atgaaaagag ggacagccgt caattataaa aatcataaag
    18481 aaaataccca tggagtctgg tttactgaac ttgaaggaaa ctataattac caagcatata
    18541 cttatcgagt ctactataga cgacgtacct ttaaaattac gagagaccct tattccattg
    18601 caacgactgc aaatggtaaa aggtctattg ttattgctcc agaagcatta actccggaag
    18661 ggtttaaaat aagtcatgga aaagaagcta agtggcggct cgaaaacccc aatcaagcag
    18721 tcatttacga aatgcatgtg cgtgattttt cgatatctga gacttcagga gttaagacag
    18781 attatcacgg aaaatttaag ggattgcatc aaaaaggtac ggttaatcag catggtgata
    18841 aaacgacctt tgattatgta caagacctag gagttaacta tatccaactt caaccaattt
    18901 tcgatcatca tcagacattt gatgatgatg gtcactatgc ttataattgg ggatatgatc
    18961 ctgaaaacta caatgttcca gaagcaagct tctctagtaa tccacatgaa cctgcaacgc
    19021 gtattttaga attaaaatct gctatccaag cgtaccatga tgcaggcatt ggtgttatta
    19081 tggatgttgt ctataatcat actttttcat caactgattc tgcatttcaa ctaacggttc
    19141 cagattatta ttatcgaatg aatcacaatg gaacatttca aaatgggtca ggctgtggta
    19201 atgaaacagc tagtgaaaaa gaaatgtgtc gtaagtatat tcttgattca gttttgtatt
    19261 gggtgaaaga atataatatt gatggttttc gatttgattt gatgggacta catgatgtag
    19321 aaacgatgaa cattataaga aatgagctaa ataaaatcga ccctcgtatt ttggtttatg
    19381 gagaaggatg ggacatggga gctggtctca cgcctcaaaa taaagctaaa aaagataatg
    19441 cttatcagat gcctggaatt ggctttttca atgatgatgt aagagacgct gtcaaaggtg
    19501 cggaaattta cggagaattt aaaaaaggct tagtttctgg aaacagtaca gaagatattg
    19561 ttgcaaaagg cattttagga agtgatgagt tagtatcata cattgatcct agtcaagttt
    19621 taaattatgt tgaagcacat gacaactaca acttaaatga tttgctatgg gaattacatc
    19681 ctaatgacaa cgagaaacaa catatttacc gagttgaagt agctagtgct atgaatctat
    19741 tgatgcaagg gatggctttc atgcaattag gtcaggagtt tcttcgaaca aagtgttatc
    19801 ctacaggtga taaaggtcaa cttactcaag cagataaaga aagggctatg aattcttaca
    19861 atgccccaga ccaagttaat caggtcaact gggataatgt tacctttcat aagagtacta
    19921 ttaattttat taggaaaata attactttga agacaaactc tccttacttt tcttattcta
    19981 gctttgaaga gattcgcaaa catgtttttg tagagtctgc tcaataccat tcaggtttca
    20041 taagttttac agtcgaagaa cataagaaaa taaaaataat tttcaatatt aaaggaaaac
    20101 gcttgcagaa ttctttcata ggtgatataa taatatcaaa tcatgaagaa ggaaaagcga
    20161 tagattcatt agatgatgag ctttctgtaa tgatccttga tatcacaaaa taacctgaaa
    20221 acattcaggt tatttttgtg tatattaagg aatttgattt gaatttgagt ttcttatatt
    20281 ttagttaatt tttcggaaat atagagggtg aaatattttc agatgcgtct gtatgaggaa
    20341 aatgtattcc tctttttttg taccattgag gaaatatttt tgtcttatat tgactagtag
    20401 gacctgtgag gtgagtagat gtccaataaa gatgagttat atacttttgg aataggtgaa
    20461 aattttcatc tccagaatta tttaggcgta catagcgaaa atggaagctt ttgtttcaga
    20521 gtttgggctc ctaatgctga aaatgttcaa gttattggag attttacaga ttggcgaaat
    20581 cgacctctcc aaatgaacaa aaatcaagca ggagtttggg aagctaacag tttggatgcg
    20641 cgcgaaggtg atttatataa atatttggtt actcgtaaag gtggtcaagt agttgagaaa
    20701 atcgatccta tggcagttta tatggaaaga cgtcctggaa ctgcaagtgt tattaaagtt
    20761 cttagaaata aaaagtggga agatggtctt tggatgggaa ggcgcaaacg tctgggtttt
    20821 cagaagcgtc caattaatat ttacgaagtt catgctggtt catggaaaaa agatgatttt
    20881 ggacatccta tgactttttc tcagttaaaa gattacttaa tcccctacct tgttgagatg
    20941 aattatactc atgttgaatt tatgccctta atggcacatc cgcttgatat gagttgggga
    21001 tatcaattaa tggggtattt tgcttttgaa catacctatg gtactccaga agaatttcaa
    21061 gactttgtag aagcatgtca taaaaataat ataggagttc ttgtagattg ggttccagga
    21121 cactttattc agaatgacga tgctttagcc tatttcgatg gaactgctac ttatgagtat
    21181 caaaatcatg atcgtgcgca taactatcga tggggagctc tgaactttga tttaggaaaa
    21241 aatcaagtac aatccttttt gatttcgagt gccttgttct ggattgaaca ttatcatatt
    21301 gatggtattc gtgttgatgc ggttagtaat atgttatatc tcgattatga tgaaggacca
    21361 tgggaagcta accaatttgg ggataatcgt aatttggaag gatatcactt tttaaggaaa
    21421 cttaacaaag taattaaaga gcgtcatcct aatgttatga tgattgctga agagtcaaca
    21481 gcttctaccc caataactaa agacttagag tctggaggat tgggttttga ctttaaatgg
    21541 aatatgggct ggatgaatga tatcctgcgt ttttatgaag aagatcctct ctataggcaa
    21601 tatgatttta atttagtgac ttttagtttt atgtatatat tcaatgagaa ctttgtacta
    21661 gctttttcac atgatgaagt agtacatggt aaaaagagta tgatgcataa aatgtggggt
    21721 gaccgttata atcaatttgc tggtttacgt aatttatatg catatcaaat gtgtcatcct
    21781 gggaaaaaac ttctctttat gggaagtgag tttggtcaat ttttagaatg gaaatataat
    21841 gatcaactag aatgggaaaa cttaaatgat gatatgaacc aaaagatgca acgctatact
    21901 aaacaattga atcaatttta taaagatcat aagtgcctat ggcgtattga tgattccttt
    21961 gacgggctag aaattattga tgcggataat aaatctgaaa ctgttttatc ttttatacga
    22021 aaagatgata aaggcgattt gttactttgt gtctttaata tgactcctgt agaacgccct
    22081 aactttacta ttggtgttcc gcaagcgggt atttatgaag aagttttaaa tactgaaatg
    22141 gaagagtttg gaggtgtttg gaaaaaccac aatcctgtaa cgaaaacaca agtggctaca
    22201 tggaaagatt atgatcatac tttaagtttt acattaccag cccttggggc aagtgtttgg
    22261 agaataaaac gtcggcttcg taaataagtc tgcaatttag taacctcatt ttgaaaggaa
    22321 agaataatat gaaaaatgaa atgttagcac tcattctcgc cggaggacaa ggcactcgac
    22381 tcggaaaatt gactcagagc attgcgaaac ctgctgttca atttggaggt cgttaccgta
    22441 ttattgactt tgcgctttca aattgtgcga attcaggtat taataatgtt ggagttatta
    22501 ctcagtatca accgctggaa ctcaatactc atattggaaa cggatctagc tggggactag
    22561 atggtatcga ttcgggagta actgtattac agccatattc tgcaacagaa ggtaatcgct
    22621 ggtttcaagg taccagccac gctatctatc aaaatattga ctacattgat cgtattaatc
    22681 cagaatatgt actcattctc tctggggacc acatctataa aatgaactat gatgacatgc
    22741 tacagacgca taaagataat ttagcaagcc taacagtcgc tgttttagat gttcctttaa
    22801 aagaagcaag ccgttttgga attatgaata ctgattcaaa cgaccgtatt gtcgaatttg
    22861 aagaaaaacc tgaacatcct aagtcaacaa aggcttcaat gggaatatac atttttgact
    22921 ggaagagatt acggacagtt ttaattgatg gtgaaaagaa cggcatagac atgtcagact
    22981 ttggtaaaaa tgttatacca gcataccttg aatcgggtga acgtgtttat acctataatt
    23041 ttgatgggta ttggaaagat gttggtacta ttgaatccct ttgggaagct aatatggaat
    23101 atattggaga agataacaaa ttacatagtc gtgatcgttc atggaaaatc tattctaaaa
    23161 atttgattgc accacctaat ttcatgacag aagatgcaaa tgttaaagat tctttagttg
    23221 tagatggatg ctttgttgca ggaaatgttg agcactccat ttcatcaact aatgtccagg
    23281 taaaaccaaa tgctattatt aaagactcct ttgttatgag tggcgcgact atcggtgaag
    23341 gtgcaaagat aaatcgagct attatcggtg aggatgctgt tattggtgat ggtgttgtga
    23401 ttgatggaag taaagaagta gaagttattg gttataaaga agtagcgggg gttccaaatg
    23461 aagattgata aatatactgc gattttagga aatactgtta gttacccaga tatgcacagt
    23521 ttgacggata atcgtccgct tgcttctcta ccttttgatg gtaagtatcg tttaattgat
    23581 tttcaattat cctctttagc taatgcaggc gttagaagta tt
//
LOCUS       DATRMA010000025        23380 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.025, whole genome shotgun sequence.
ACCESSION   DATRMA010000025 DATRMA010000000
VERSION     DATRMA010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 23380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23380
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.025"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            7..885
                     /locus_tag="VBC28_000946"
     CDS             7..885
                     /locus_tag="VBC28_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEO6814946.1"
                     /translation="MRESIFEETAKGKVSRHVLLTILLALILLFTADFLTYIIIELII
                     SLDFWKLEWDSDIVQLIILLATSISTVMLFMWVKFKEKRPISSLGLFKEGALTELSRG
                     WLVGSVLFGACLILTIISGSGVIETVRFTADKLIWFLIFAVGWQVQSASVEILCRGWL
                     LPVSATKYSKIVSVSISSIFFGLLHSANNHVSLISIFNLCLFGLFLSLYVILKGNIWG
                     ACGIHGAWNCVQGSVFGIEVSGEPMLSNSLVHVKTYGADWISGGKFGVEGSMITSIVL
                     IVACYWLYQKSNFPYD"
     gene            992..1582
                     /locus_tag="VBC28_000947"
     CDS             992..1582
                     /locus_tag="VBC28_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEO6814947.1"
                     /translation="MPQNPTSNPEKKTYLLSELVDCFKGKAVPSKAEAGDIRIINLSD
                     MSPLGIDYHNLRTFQDEQRSLLKYLLQEGDVLIASKGTVKKVAIFEEQDYPVVASANI
                     TILRPTQHIRGYYLKLFFDSEEGQQALENANKGKAVMNISTKELLNIAIPSIPLFRQD
                     YLIQRYKQGLNDYKRKIARAEQEWERIQNDIRQQLS"
     gene            1616..2887
                     /locus_tag="VBC28_000948"
     CDS             1616..2887
                     /locus_tag="VBC28_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HEO6814948.1"
                     /translation="MNEIKCPHCGTAFAINESEYHQLLEQIRGDAFDKEVSERLEKER
                     LILGEQAKNQLQEVVVEKDKEIAKLQYKVKQFLIEKDNLLKDNEYQLAEQLNQKDMML
                     RDLENQIDRLRLEHENSLQEALTKVERERDAIQNQLHIQEKEKDLALASVKSDYEVQL
                     KAANEQVEFYKNFKAQQSTKAVGESLEHYAETEFNKVRHLAFPNAYFEKDNTLSSRGS
                     KGDFIYREKDENDLEFLSIMFEMKNESDDTIKKHKNEDFFKELDKDRREKSCEYAVLV
                     TMLEADNDYYNTGIVDVSHKYPKMYVIRPQFFIQLIGILRNAALNTLKYKQELALMKE
                     QNIDITHFEEDLDIFKNAFAKNYNSASKNFQKAIDEIDKSIKRMEAVKAALTTSENQL
                     RLANNKLDDVSVKKLTRKNPTMKAKFDALKD"
     gene            2900..3331
                     /locus_tag="VBC28_000949"
     CDS             2900..3331
                     /locus_tag="VBC28_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO6814949.1"
                     /translation="MKIRTATLDDSEKLVPLYQELGYAISLSEIQSILKVILTHSDYG
                     FLIAEDNGKLLAFVGYHKLYFFEKSGTYYRILALVVNEKHRRKGIASQLINHVKQLAK
                     TDGSEVLALNSSLKEYRQEAYHFYENLGFKKVSTGFSYYLD"
     gene            3423..4307
                     /gene="truB"
                     /locus_tag="VBC28_000950"
     CDS             3423..4307
                     /gene="truB"
                     /locus_tag="VBC28_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001876923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEO6814950.1"
                     /translation="MITGIINLKKEAGMTSHDAVFKLRKILHTKKIGHGGTLDPDVVG
                     VLPIAVGKATRVIEYMTESGKIYEGEITLGYATSTEDSSGEVISRTPLTQSDLSEDVV
                     DHAMKSFTGPITQVPPMYSAVKVNGKKLYEYARSGEEVERPKRQITISEFRRTSPLYF
                     EKGICRFSFYVSCSKGTYVRTLAVDLGIKLGYASHMSFLKRTSSAGLSITQSLTLEEI
                     NEKYKQEDFSFLLPIEYGVLDLPKVNLTEEDKVEISYGRRILLENEADTLAAFYENRV
                     IAILEKRGNEFKPHKVLL"
     gene            4320..5252
                     /locus_tag="VBC28_000951"
     CDS             4320..5252
                     /locus_tag="VBC28_000951"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEO6814951.1"
                     /translation="MKIHYINDYKDIQAKEDCVLVLGYFDGLHLGHKALFDKAKKIAT
                     EKNLKIVVLTFNETPRLTFARFQPELLLHLTSPEKRSEKFQEYGVDELYLMNFTSHFS
                     KVSSDLFIKKYIYGLRAKAAVVGFDYKFGHNRTSGDYLARNFKGPVYIIDEISEGGEK
                     ISSTRIRQLITEGNVEKANQLLGYEFSTCGMVVHGDARGRTIGFPTANLAPINRTYLP
                     ADGVYISNVLINGKYYRAMTSIGKNITFGGTELRLEANIFDFDGDIYGETIEIFWLKR
                     IREMVKFNGIDDLVKQLKKDKEIALNWKKDSQTL"
     gene            5295..5708
                     /locus_tag="VBC28_000952"
     CDS             5295..5708
                     /locus_tag="VBC28_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000631264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HEO6814952.1"
                     /translation="MITLFLSPSCTSCRKARAWLSKHEVAFEEHNIITSPLNKEELLQ
                     ILSFTENGTEDIISTRSKVFQKLAIDVDELSTSSLMELISENPSLLRRPIILDKKRMQ
                     IGFNEDEIRAFLPRDYRKQELKQATIRAEIEGKHD"
     gene            5701..5982
                     /locus_tag="VBC28_000953"
     CDS             5701..5982
                     /locus_tag="VBC28_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HEO6814953.1"
                     /translation="MISSNYSYPLDPSWNTEDITKVLRFLNQVEHAYENSIKVDDLLD
                     SYKEFKKVVKSKAQEKQIDREFQRTSGYSTYQAVKAAQQQAKGFISLGR"
     gene            5972..6736
                     /locus_tag="VBC28_000954"
     CDS             5972..6736
                     /locus_tag="VBC28_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEO6814954.1"
                     /translation="MDAKFDFAKQLVYKAGQFIKSEMQNTFDVEEKSRFDDLVTSLDK
                     KTQKLLIQEIIQHYPDDNILAEEDDVRSPIAQGNVWVLDPIDGTVNFIVQKDNFAVML
                     AYYEEGVGQFGIIYDVMADILYSGGGHFDVYANDKKIVPFQECPLERCLLGVNSAMYA
                     ENDCGIAHLASETLGVRIYGGAGISMAKVMQGKLLAYFSYIQPWDYAAAKIMGETLGF
                     TLLTLDGEEPNYSTRQKVMFLPKSKLNLIQSYLTKK"
     gene            6804..8114
                     /locus_tag="VBC28_000955"
     CDS             6804..8114
                     /locus_tag="VBC28_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HEO6814955.1"
                     /translation="MKLPNEFIEKYQTILKDEAEAFFDSFEQKPISAYRTNPLKEKQL
                     DFPNAIPSTPWGHYGKISGKSIEHTTGLVYSQEPAAQIVAQIAEPQEGMKVLDLAAAP
                     GGKTTHLLSYLNNTGLLVSNEISNKRSKILVENVERFGARNVIVTNESSQRLAKCFNS
                     FFDLIVFDGPCSGEGMFRKDPQAIQYWHKDYPTECAQLQRDILKEAIKMLAHGGILVY
                     STCTWSPEENEEVVNWLLQEYDYLELVDIPKLNGMVEGINVPQVARMYPHHFQGEGQF
                     VAKLRDTRSKEAQKIKPKAQKINKMQLQLWQQFAQDHLKIDLNGVLDVFGDQLYLLPN
                     GLPDLSKLKIARNGLHLGTFKKNRFEPSFALGMALSEHDLVQSIEIDIEQFEVYVSGN
                     VVKLDKTVPNGWYQILVKGNGLGFAKVTNNTLKNYYPKGLRFQT"
     gene            8293..9159
                     /locus_tag="VBC28_000956"
     CDS             8293..9159
                     /locus_tag="VBC28_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HEO6814956.1"
                     /translation="MKMKKNIFLLTLLATVLLTLSGCANWIDKGQSITSVGSTALQPL
                     VEAAADEFGKTNLGKTINVQGGGSGTGLSQVQSGAVQIGNSDLFAEEKDGIDAKKLKD
                     HQVAVAGLAVIVNKKVNVKNLTTHQLRDIFAGKIKNWKEVGGQDLDISIINRAASSGS
                     RATFDNTIMGNVAPIQSQEQDSNGMVKSIVSQTPGAISYLAFAYVDKSVGTLKLNGFA
                     PTAKNVTTDNWKLWSYEHMYTKGNETGLTKEFLDYMKSDKVQSSIVQHMGYISINDMK
                     VVKDAEGKVTPK"
     gene            9205..10122
                     /gene="pstC"
                     /locus_tag="VBC28_000957"
     CDS             9205..10122
                     /gene="pstC"
                     /locus_tag="VBC28_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEO6814957.1"
                     /translation="MKNQELAKKLTSPSKNSRLEKFGKGITFLSLALIVFIVAMILIF
                     VAQKGLSTFFVDGVKLTDFLFNTKWEPSAKSFGAFPMIAGSFIVTILSAIIATPFAIG
                     AAVFMTEISPKYGSKILQPAVELLVGIPSVVYGFIGLQIIVPFVRSIFGGTGFGILSG
                     VCVLFVMILPTVTFMTVDSLRAVPRHYKEASLAMGATRWQTIWRVILNAARPGIFTAI
                     VFGMARAFGEALAIQMVVGNSAILPTSLTTPAATLTSVLTMGIGNTVMGTVQNNVLWS
                     LALVLLIMSLAFNTVIKLITREGKKNYER"
     gene            10112..10999
                     /gene="pstA"
                     /locus_tag="VBC28_000958"
     CDS             10112..10999
                     /gene="pstA"
                     /locus_tag="VBC28_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000992189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEO6814958.1"
                     /translation="MNAKKADKLATTILYSIAAIIVTILASLLIFILVRGLPHVSWSF
                     LTGKSSSYEAGGGIGIQLYNSFFLLIVTLIISIPLSLGAGIYLSEYAKKGRLTNFVRT
                     CIEILSSLPSVVVGLFGYLIFVVQFQYGFSIISGALALTVFNLPQMTRSVEDSLQNVH
                     HTQREAGLALGISRWETVIYVVVPEALPSIVTGVVLASGRIFGEAAALIYTAGQSAPA
                     LDWSNWNVLSVTSPISIFRQAETLAVHIWKVNSEGTIPDATQVSAGSAAVLLVVILIF
                     NLSARSIGKKLHSKLTSSN"
     gene            11011..11814
                     /gene="pstB"
                     /locus_tag="VBC28_000959"
     CDS             11011..11814
                     /gene="pstB"
                     /locus_tag="VBC28_000959"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEO6814959.1"
                     /translation="MAEYNWDERHIITFPEENSALTTKDLHVYYGEKEAIKGIDMQFE
                     KNKITALIGPSGCGKSTYLRSLNRMNDTIDIARVTGQIMYEGIDVNAQDINVYEMRKH
                     IGMVFQRPNPFAKSIYKNITFAYERAGVKDKKFLDEVVETSLKQAALWDQVKDDLHKS
                     AFTLSGGQQQRLCIARAIAVKPEILLMDEPASALDPIATMQLEETMFELKKNYTIIIV
                     THNMQQAARASDYTAFFYLGDLIEYDKTNNIFQNAKCQSTSDYVSGRFG"
     gene            11826..12584
                     /gene="pstB"
                     /locus_tag="VBC28_000960"
     CDS             11826..12584
                     /gene="pstB"
                     /locus_tag="VBC28_000960"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEO6814960.1"
                     /translation="MTQPILQVSDLSVYYNKKKALKEVSMDFYPNEITALIGPSGSGK
                     STLLRAINRMGDLNPEVTLTGAVMYNGHNVYSPRTDTVELRKEIGMVFQQPNPFPMSV
                     FENVVYGLRLKGIKDKATLDEAVETSLKGASIWDEVKDRLHDSALGLSGGQQQRVCIA
                     RTLATKPKIILLDEPTSALDPISAGKIEETLHGLKDQYTMLLVTRSMQQASRISDRTG
                     FFLDGNLIEYGNTKEMFMNPKHKETEDYITGKFG"
     gene            12618..13271
                     /gene="phoU"
                     /locus_tag="VBC28_000961"
     CDS             12618..13271
                     /gene="phoU"
                     /locus_tag="VBC28_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEO6814961.1"
                     /translation="MLRSKFDEELDKLHNQFYAMGIEAIGQIKKTVRAFVSHDRELAK
                     EVIEDDVTLNNFETKLEKKSLEIIALQQPVSQDLRTVITVLKATSDVERMGDHAAAVA
                     KATIRMKGEERIPAVELEINNMGKAVKNMLEEALTAYINGDDEKAYEVAAMDEIVDDY
                     FRDIQKMVVETIQKHPDVAFAAKEYFQVLMHLERIGDYGKNICEWIVYLKTGKIIEL"
     gene            13417..15966
                     /locus_tag="VBC28_000962"
     CDS             13417..15966
                     /locus_tag="VBC28_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEO6814962.1"
                     /translation="MKTVEHFVTKFVPENYNLFLDINRQTKTFSGNVAVSGEALDNNI
                     SFHQKGLTIKSVLLDNQPLDFQLDEDNEAMHIQLHETGSMVLVFEFSGHITDNMTGMY
                     PSYYTVNGIKKEVISTQFESHFAREVFPSIDEPEAKATFDLSLKFDQKEGEIALSNMP
                     EINAEQRQETGLWTFDTTPKMSSYLLAFALGELHGKTTHTKNGTLVGSYATKAHQLNE
                     LDFSLDIVVRVIEFYEDYFGVRYPIPQSLHVALPDFSAGAMENWGLVTYREVYLLVDE
                     NSSVSSRQQVALVVAHEIAHQWFGNLVTMKWWDDLWLNESFANMMEYVSIDYIEPKLN
                     IFEDFQTGGLPLALKRDATDGVQSVHVEVNHPDEINTLFDPAIVYAKGSRLMHMLRRW
                     LGDTDFAAGLKIYFEKHQYQNTIGRDLWNALSQTSGKDVAAFMDSWLEQPGYPVMAAK
                     IEEDELILTQKQFFIGEHEDKSRLWQIPLNSNWEGIPEILTEETVVIPNFSQLAEKNK
                     ENGALRFNTENTAHYITNYQGQLIEHIISDLPLMDNISKLQIVQERHLLAESGMISYS
                     SLIPLVSLLSQETSYLVNSAIKSVIDGLSLFVQEDSQDEFDFKEFVNKLSAFNFNRLG
                     FEKREGEGDDSEMVRHLSLSLALYSDNEHAIEEAHHIFKAHENNIAAIPAAIRLLVLT
                     NEMKHFESKELSHLLLETYSTTTDGNFKRQLASALSHTTDSKTLKKLLSDWKNKDIVK
                     PQDLAMSWYATFLKNSFTQESVWEWAQENWEWIKATLGGDMSFDKFVIYPSSSFKTEE
                     RLEQYKNFFEPQLSDMAISRNISMGIKEISARVLLITKQKEEVINTIKKYI"
     gene            16128..16808
                     /locus_tag="VBC28_000963"
     CDS             16128..16808
                     /locus_tag="VBC28_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO6814963.1"
                     /translation="MIKILLIEDDLSLSNSVFDFLDDFADVMQIFDGEEGLYEAESGV
                     YDLILLDLMLPEKNGFQVLKELREKGITTPVLIMTAKESIDDKGQGFDLGADDYLTKP
                     FYLEELKMRIQALLKRSGKFNDNSLIYGDIRVDMSTNSTFVNQTEVELLGKEFDLLVY
                     FLQNQNVILPKSQIFDRIWGFDSDTTISVVEVYVSKVRKKLKGTLFSENLQTLRSVGY
                     ILKHVETD"
     gene            16792..18105
                     /locus_tag="VBC28_000964"
     CDS             16792..18105
                     /locus_tag="VBC28_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000042569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEO6814964.1"
                     /translation="MSKPIKISKFKKNVSDSHFIHFFTVFSGIFLVMTVIILQVMRYG
                     VYSSVDSSLKYISTHPKNYINMVMSRTAAYLDNSNIASVKLKPGGQTVANTDIILFTS
                     EEEVINYFDAFSNYQFLKPNKKNLGGISELTLTNIFGQDETYHAVTVKVNNPAYPNVT
                     YMTAIVNIDQLVNAKERYEKIIIFVMTTFWIISIGASIYLAKWAQKPIIENYERQKAF
                     VENASHELRTPLAVLQNRLETLFRKPNATILENSENIASSLDEVRNMRILTTNLLNLA
                     RRDDGIKPELAVIKPTLFDSIFENYDLITQENGKNFTGHNMIQDSFKTDKTLLKQLMT
                     ILFDNAIKYTDNDGSIDFTISETDKYLFLEIADNGPGISEEDKVRIFDRFYRVDKART
                     RQQGGFGLGLSLAQQIVNSLRGNITVIDNKPRGSIFKIKLPKVKG"
     gene            18194..18526
                     /locus_tag="VBC28_000965"
     CDS             18194..18526
                     /locus_tag="VBC28_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HEO6814965.1"
                     /translation="MEIEKTNRMNALFEFYAALLTDKQMNYIELYYADDYSLAEIAEE
                     SGVSRQAVYDNIKRTEKILEAYEMKLHMYSDYIVRSQIFDDILEKYTDDAFLQEKISI
                     LSSIDNRD"
     gene            18544..20109
                     /gene="ffh"
                     /locus_tag="VBC28_000966"
     CDS             18544..20109
                     /gene="ffh"
                     /locus_tag="VBC28_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEO6814966.1"
                     /translation="MAFESLTERLQGVFKNIRGKKKLSEKDVQEVTKEIRLALLEADV
                     ALPVVKTFIKHVRERAVGHEIIDTLDPTQQIVKIVNEELTDLLGAETSEIEKSPKIPT
                     IIMMVGLQGAGKTTFAGKLANKLIKEDNARPMMIAADIYRPAAIDQLKTLGSQINVPV
                     FDMGTNHSAVEIVTKGLEQARENRNDYVLIDTAGRLQIDATLMQELHDVKAIAQPNEI
                     LLVVDSMIGQEAANVAEEFNRQLSISGVVLTKIDGDTRGGAALSVREITGKPIKFTGT
                     GEKITDIETFHPDRMASRILGMGDLLTLIERASQEYDEKRSMELAEKMRENTFDFNDF
                     IDQLDQVQNMGPMEDLLKMLPGMANNPAMKNFKVDENEIARKRAIVSSMTPEERENPD
                     LLNPSRRRRIAAGSGNTFVDVNKFIKDFNQAKQMMQGVMSGDMNKMMKKMGIDPNNLP
                     KDMPGMDGMDMSNLEGMMGQNGMPDLSSLGGDMDFSQMFGGGLKGKVGAFAAKQSMKR
                     MANKMKKAKKKRK"
     gene            20176..20862
                     /locus_tag="VBC28_000967"
     CDS             20176..20862
                     /locus_tag="VBC28_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SatD family protein"
                     /protein_id="HEO6814967.1"
                     /translation="MYLALIGDIINSKQILERETFQQSFQQLMTELSDVYGEELISPF
                     TITAGDEFQALLKPSKKVFQIIDHIQLALKPVNVRFGLGTGNIITSINSNESIGADGP
                     AYWHARSAINHIHDKNDYGTVQVAICLDDEDQNLELTLNSLISAGDFIKSKWTTNHFQ
                     MLEHLILQDNYQEQFQHQKLAQLENIEPSALTKRLKASGLKIYLRTRTQAADLLVKSC
                     TQTKGGSYDF"
     gene            20852..21625
                     /locus_tag="VBC28_000968"
     CDS             20852..21625
                     /locus_tag="VBC28_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3307 domain-containing protein"
                     /protein_id="HEO6814968.1"
                     /translation="MISDFLRDNPILTLLFCAHFLADFQWQSQSLADSKSHSWRGLWR
                     HLLIVFLPLAALMILIPETTLLNLSIWGSHIVIDSIKKLSYPWVEEGHFQKAAFIIDQ
                     LAHYTCIIVFYHALPTYLPPNHWLLPIKHFIVIALVFIIITKPINIVFKIFFNKFQAK
                     ELSSLLTQEKTKIMKEKSEDHEETIEGAGAMIGNLERLIMAILLISGQYAAIGLVFTA
                     KSIARYDKISKSQVFAEYYLIGSLFSIISVLITHWLLLV"
     gene            complement(21658..23319)
                     /locus_tag="VBC28_000969"
     CDS             complement(21658..23319)
                     /locus_tag="VBC28_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000093963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEO6814969.1"
                     /translation="MSSFNRKKLKFLGISLATLTATTVTLVACGNESKNSGDNKVINW
                     YIPTEISTLDISKNTDAYSNLAIGNSGSNLLRIDKEGKPKPDLAKKVSVSSDGLTYTA
                     TLRDNLKWSDGSKLSAEDFVYTWRRIVDPKTASEYAYLATESHLLNADKINSGDIKDL
                     NKLGVTAKGNQVTFKLTSPCPQFKYYLAFSNFMPQKQSYVEKVGKDYGTTSKNQIYSG
                     PYLVKDWNGSNGKFKLVKNKYYWDSKHVKTNSVIVQTIKKPDTAVQMYKQGQIDFAEI
                     SGTSAIYQANKNNKDVVDASDARTTYIIYNQTGSVKALTNQKIRQALNLATDRKGVVK
                     AAVDTGSTPAESLVPKKLAKLPNGEDLSKYTAPGYTYNTSKAQKLFKEGLAEVGQSSL
                     KLTITADSDSPAAKNAVDYVKSTWESALPGLTVEEKFVTFKQRLEDAKNENFDVVLFS
                     WGGDYPEGSTFYGLFTTNSAYNYGKFSSKEYDNAYQKAITTDALKPGDAANDYKTAEK
                     ALFDQSYYNPVYYLGKKGLQNSKLKGLVRNSTGLNVDFTYAYKTE"
BASE COUNT         7908 a         3944 c         4291 g         7237 t
ORIGIN      
        1 atatttatga gagagtctat ttttgaggaa actgcaaaag gtaaagtatc aaggcatgtc
       61 cttttaacta tattattggc tttgatatta ctatttacag cagatttcct tacctatatt
      121 attattgaat tgattatctc gcttgatttt tggaaactag agtgggatag cgatatcgtc
      181 cagttaatca ttttactagc aacttcaatt agtacggtaa tgttatttat gtgggtgaaa
      241 tttaaagaga agcgtccaat tagttctcta gggctattta aagaaggagc cctaacagaa
      301 ttatctagag gatggttagt cgggagcgtt ctctttggag cctgcttaat attaacaata
      361 atttcaggct ctggagttat cgaaactgtt cgttttacag ctgataaatt gatttggttt
      421 cttatttttg ctgttggatg gcaggtacaa agcgcatctg ttgaaatatt gtgtcgtgga
      481 tggttacttc cggtgagtgc cacaaagtat agtaagatag tttctgtcag tatcagttca
      541 atctttttcg gtcttctgca tagcgctaat aatcatgtaa gccttatctc gattttcaat
      601 ctgtgtctct ttggactatt tctatcgctc tatgttattt taaaaggtaa catctggggg
      661 gcgtgtggta ttcatggcgc ctggaactgt gtacaaggga gtgtctttgg aatcgaagtt
      721 agtggagagc caatgttgtc taactcccta gtccatgtta aaacctatgg tgctgactgg
      781 attagcggtg gaaaatttgg tgtggaagga agcatgatta ccagtattgt tcttatcgta
      841 gcatgttatt ggctttatca aaaatcgaat tttccttacg actaatagat ttaatctaaa
      901 taagcttctc ttactgaaag cttatttttt attttagtat taaaaaacta tgttttgtga
      961 tataatattt taaagacgaa aggagtctat tgtgccacaa aatccaacaa gtaacccaga
     1021 gaaaaaaact tatttattat ctgaattggt agactgcttt aaaggtaaag ctgtgccttc
     1081 aaaagcagaa gcaggtgata tccgtattat taatttgagt gatatgtctc ctttaggaat
     1141 tgactaccat aacttaagaa cattccaaga tgaacaacga tcgcttttaa aatatctact
     1201 tcaggaaggc gatgttttaa tcgcttcaaa aggaactgtc aagaaagttg ctatttttga
     1261 agagcaagat taccctgtcg ttgcctctgc taatattaca atactaaggc ctacacaaca
     1321 cattagaggg tactacttaa agcttttttt tgattcagaa gaaggtcaac aggcacttga
     1381 gaatgctaac aaaggaaaag ctgtgatgaa tattagcaca aaggaactgt taaatattgc
     1441 aataccaagt atcccgcttt ttagacagga ttatcttatc caacgttata aacaggggct
     1501 caacgattac aaacgtaaga tagcacgtgc agagcaggag tgggaacgta tccaaaatga
     1561 catcagacaa caacttagct agaaccaaat acaaaacatc ataggaagag ttatcatgaa
     1621 cgaaatcaaa tgccctcatt gtggaacagc ttttgccatc aacgagtctg aataccatca
     1681 attactagaa caaattcgcg gagatgcttt tgacaaagaa gtaagtgaac ggttggaaaa
     1741 agaacgtcta atattagggg agcaagcaaa aaatcaatta caggaagttg ttgtagaaaa
     1801 agacaaggag atagctaaac ttcagtacaa agtcaaacaa tttcttatag aaaaagacaa
     1861 tcttctcaaa gacaatgagt accaactcgc tgagcaatta aatcaaaaag acatgatgct
     1921 tcgcgacctt gaaaaccaaa tcgatagact acgtttagag catgaaaata gcttgcaaga
     1981 ggcgctaaca aaagtcgaac gagaaagaga tgcaatacaa aatcagttgc acattcaaga
     2041 aaaagaaaaa gatttagctt tagcttcagt aaaaagtgat tatgaagtac aactaaaggc
     2101 agccaatgaa caagtagaat tctataaaaa cttcaaagct caacagtcta ctaaagcagt
     2161 aggagaaagt ttagaacatt atgctgaaac agaatttaat aaagtgcgac atttggcctt
     2221 tcctaatgct tattttgaga aggacaatac attatcaagt cgtggctcaa agggggactt
     2281 tatctatcga gaaaaggatg aaaatgacct tgagttttta agtatcatgt ttgaaatgaa
     2341 aaatgagtct gatgatacta tcaagaagca taaaaatgaa gattttttca aagaattaga
     2401 taaagatcgt cgtgaaaaat cttgcgaata cgcagtttta gtaactatgc ttgaagcaga
     2461 caatgactat tataatactg gaattgttga tgttagtcac aaatacccta aaatgtacgt
     2521 tatacgtcca caatttttta tccaattaat tggtattcta agaaatgcag cactcaatac
     2581 cttaaaatat aaacaagagc ttgctttgat gaaagaacaa aatattgaca tcacacattt
     2641 tgaagaagat ttagatattt tcaaaaatgc atttgctaaa aattataatt ctgcaagcaa
     2701 aaatttccag aaagcaatcg atgaaataga taaatctatt aaacgtatgg aagctgttaa
     2761 ggctgcttta acaacgtctg aaaatcaact acgtcttgca aataataaat tagacgatgt
     2821 ttctgtcaag aaattaacaa gaaaaaatcc aacaatgaaa gcaaaattcg atgctctaaa
     2881 agactaagag gttatgatta tgaagattag aactgctaca ctagacgata gtgaaaaatt
     2941 agtccctttg tatcaagaat taggatacgc aatatcatta tcagagattc aatctatttt
     3001 aaaggttatt cttactcatt cagattatgg ctttttgata gctgaggata atggcaaact
     3061 cctcgccttc gttggatatc ataaacttta cttttttgaa aaaagtggga cttactatcg
     3121 tattttagca ctagtagtta atgaaaagca ccgtcgtaaa ggcatcgcta gtcaacttat
     3181 taatcacgtt aaacagctag ctaaaacaga tggaagtgaa gtactggctt taaatagcag
     3241 tttaaaagaa tatcgtcagg aagcttatca tttttatgaa aacctagggt tcaagaaggt
     3301 tagtacaggc ttttcatact atttagatta ggtataaatc cttatagcac tataccaaaa
     3361 gagcgtaagt ttttacgctc ctttctttgt gttaccgctt atttactgat ataatataaa
     3421 acatgattac aggaataatt aatttaaaga aagaagctgg aatgacatca cacgatgctg
     3481 tattcaagct tcgaaagata ttacatacaa aaaaaatagg acacggcgga actcttgatc
     3541 cagatgttgt tggggtttta ccaattgcgg ttggaaaagc cactcgtgtt attgaatata
     3601 tgacagaaag tggcaaaatt tatgaaggag aaatcacttt aggatatgcc acaagtacag
     3661 aagattctag tggagaggtc atttcaagaa ctccactaac tcaatccgat cttagcgagg
     3721 atgttgtgga tcatgcgatg aaaagtttta ccggtccaat tacacaagtt cctcctatgt
     3781 attcagcagt taaagtaaat ggtaaaaaac tctatgaata tgctcgttca ggagaagaag
     3841 tagagcgacc taaacgacaa ataacaattt ctgaatttag acgcacaagc ccattgtact
     3901 tcgaaaaagg catctgccgt tttagtttct atgtctcatg cagtaaaggt acctatgtta
     3961 ggacgttagc tgttgatcta ggaataaaac taggatatgc tagccatatg tctttcctaa
     4021 aaagaacctc atctgcagga ttatctatta cacaatcatt aacgcttgaa gaaattaatg
     4081 aaaaatataa gcaagaagat ttttcatttt tacttccaat tgaatatggt gttttagatc
     4141 tcccaaaagt taacctaact gaggaagata aagttgaaat ctcatacgga cgtcgtatct
     4201 tattagaaaa tgaagcagat accttagcag ctttttatga aaatagagtt atcgctatac
     4261 tagaaaaacg tggcaatgaa tttaaaccac acaaagtatt gttataaaga aagtcaatca
     4321 tgaaaatcca ttacattaat gattataaag atattcaagc taaagaagat tgcgtcttag
     4381 ttttgggcta ttttgatggc ttacatctgg gacataaagc cctctttgat aaagctaaaa
     4441 aaatcgcgac cgaaaaaaat ttgaaaattg tcgtcttaac gtttaatgag acacctcgtc
     4501 taaccttcgc tcgcttccaa cctgaattat tgctacatct tacctctcca gaaaaacgtt
     4561 ctgaaaaatt tcaagagtat ggcgtcgatg agttatattt aatgaatttt accagtcatt
     4621 tttcaaaagt atcttctgac ctttttatta agaaatatat ttatggtctc agagcaaaag
     4681 ctgctgttgt cggttttgat tataagttcg gtcataatcg taccagtggt gattatctag
     4741 cgcgtaattt caagggacca gtctatatta ttgatgaaat ttctgaaggt ggtgaaaaaa
     4801 tctcttctac acgtattcgt caattgatta cagagggtaa tgttgagaaa gctaaccaac
     4861 ttttgggtta tgagttttca acctgtggca tggtagtaca tggagatgct agaggacgaa
     4921 ctatagggtt cccaactgct aatctagctc ctattaatcg cacttactta cctgcagacg
     4981 gtgtttatat ttccaatgtt ctgattaatg gaaaatatta tagagcaatg acgagcattg
     5041 gaaaaaatat cacttttggt ggtacagaac ttcgtttaga agctaatatc tttgattttg
     5101 atggcgacat ttatggagaa actattgaaa ttttctggtt aaaaagaatc agagaaatgg
     5161 taaagtttaa tggtattgac gatcttgtaa aacaacttaa aaaagataaa gaaattgctt
     5221 taaattggaa aaaagatagt caaactcttt aattttttgt ataatagtct ctagtaaata
     5281 caagaggcgg ttatatgatt accttatttt tatcacccag ctgtactagt tgtcgaaaag
     5341 cgcgtgcttg gctaagtaag catgaggttg cttttgaaga gcataacatt attacaagtc
     5401 cactcaataa agaagaacta ttacaaattc tatcgtttac agaaaatggt acagaagaca
     5461 ttatttccac acgttcaaaa gtctttcaaa aacttgctat tgatgtagac gaattatcaa
     5521 cgtcttcatt aatggaatta atttcagaaa atccaagtct tttacgacgc ccaattattt
     5581 tagacaaaaa acgtatgcag attggtttta atgaggatga aattcgtgcc ttcttaccgc
     5641 gtgactatcg taaacaagaa ctgaaacagg ccactattcg tgctgaaatt gagggaaaac
     5701 atgattagta gtaattatag ttatcctctt gatccttcat ggaatacgga agatattact
     5761 aaggtacttc gctttttaaa tcaagttgaa cacgcctatg aaaatagtat taaagttgat
     5821 gatttgctag atagctataa agaatttaaa aaagttgtta aatccaaggc gcaggaaaaa
     5881 caaattgacc gtgaattcca gagaactagc ggttattcca cttatcaagc tgttaaagca
     5941 gcacaacaac aagcgaaagg atttatttca cttggacgct aaatttgatt tcgctaaaca
     6001 attagtgtat aaagctggtc aattcattaa atctgagatg caaaatactt ttgatgttga
     6061 ggaaaagtct cgatttgatg atttagtgac aagtcttgat aaaaaaacac aaaaattatt
     6121 aattcaagaa attattcaac attacccaga tgataatata cttgccgaag aagatgatgt
     6181 gcgctcaccc attgcacaag gtaatgtttg ggttttagac cctattgatg gaactgttaa
     6241 ttttattgtt caaaaagata actttgctgt tatgttagct tattacgaag aaggcgttgg
     6301 acaatttggt atcatctacg acgttatggc tgatattctt tacagcgggg gaggacattt
     6361 tgatgtctat gctaacgata aaaaaattgt tccttttcaa gagtgtcctt tagaacgttg
     6421 cttacttgga gtaaattctg ctatgtatgc agaaaatgat tgcggtattg cccatttagc
     6481 ttctgaaacc ttaggtgttc gtatctatgg tggtgctgga attagcatgg caaaagtcat
     6541 gcagggcaaa ttattagctt atttttcgta tattcaacct tgggattatg ctgcagcaaa
     6601 aatcatgggg gagacattag gatttacctt attaacgctt gacggggaag aacccaacta
     6661 ttcaacaaga caaaaagtaa tgttcctacc aaagtcgaaa ttaaatctta tccaatccta
     6721 ccttaccaaa aaataaactt aaaaccttct aattaaatag aaggtttttt tgcacgcccg
     6781 tgattatgta gtataataga aacatgaaat taccgaatga atttattgaa aaatatcaga
     6841 ctatacttaa agatgaagca gaagcttttt ttgacagttt tgaacaaaaa cccatatcgg
     6901 catatcgcac caatcctctc aaagaaaagc aacttgattt tccaaatgcc atcccaagta
     6961 ccccttgggg gcattatggg aaaatatcag gaaaatcaat agaacacaca actggccttg
     7021 tttattctca agaacctgct gctcagatag tggctcagat tgctgaacca caagaaggaa
     7081 tgaaagtgct agatttagca gctgcacctg gtggaaaaac aacacacctt ctatcatatt
     7141 taaacaatac tggtctatta gttagtaatg aaatttcaaa taaacgtagt aaaattttag
     7201 tcgaaaatgt cgaacgtttt ggtgctagaa atgttatcgt gactaatgaa agctcccaac
     7261 gactagccaa atgttttaat tctttttttg atcttattgt ttttgacggt ccgtgttctg
     7321 gtgagggaat gttccgcaaa gatcctcaag caatacaata ttggcacaaa gattacccca
     7381 ctgaatgtgc acagttacaa agagatattt taaaagaagc aatcaaaatg ctagctcatg
     7441 gtggtatact tgtatactct acttgtacat ggtcaccaga agagaacgaa gaagtggtta
     7501 attggctcct tcaagagtat gattacttag aacttgttga tattcctaaa ctaaatggga
     7561 tggttgaagg gattaatgta ccacaagttg caaggatgta ccctcatcat tttcaaggtg
     7621 aaggacaatt cgttgcaaaa ctgagagata ctagatctaa ggaagcacag aaaattaagc
     7681 caaaagcaca gaaaataaat aaaatgcagt tacaattgtg gcaacaattt gcacaagacc
     7741 atttaaagat agacttgaac ggtgttttag atgttttcgg cgaccaactc tatcttctac
     7801 ctaatggtct ccccgatttg tctaaattaa aaatagcacg caatggtctc catctaggca
     7861 ctttcaagaa gaatcgtttt gagccatcgt ttgctttagg aatggcactt agcgaacatg
     7921 acctagtaca gtctattgaa attgatatag aacagtttga ggtgtacgta tctggaaatg
     7981 tagtcaaact agacaagact gttccaaacg gttggtatca aattcttgta aaaggcaatg
     8041 gattaggttt tgcaaaagtg acaaataata ctcttaaaaa ttattaccct aaaggactac
     8101 gatttcagac ataactgtta ttgaatactt ttaataaaaa aggaaaaaga aatacttttc
     8161 aaaccctaga aacttatggt actatgggac agtgatgtgt gtttttacta tttgtaaatc
     8221 attaaacact caatgatttt agtaatatat atcaactact aaatatcaaa atttattcaa
     8281 aagaatagga aaatgaaaat gaaaaaaaac atttttttgt taaccttgct agccaccgtc
     8341 ttattgacgc tgtctggttg tgctaattgg attgataaag gtcaatcaat caccagcgtt
     8401 ggttcaaccg ccttacagcc acttgtagaa gctgcagcag atgagtttgg taagacaaac
     8461 ctcggtaaga ctatcaatgt ccaaggagga ggctcaggaa caggcttatc acaagtacag
     8521 tctggagctg ttcaaattgg taatagcgac ttatttgcag aagaaaaaga tggtattgat
     8581 gctaagaaat tgaaagacca tcaagtcgca gtagctggat tagctgttat tgttaataaa
     8641 aaagttaatg ttaaaaatct aaccacccac caacttcgtg atatttttgc aggaaaaatc
     8701 aagaattgga aagaagtggg aggacaagat ttagatattt ccatcatcaa tcgagcagca
     8761 agttctggat cccgtgcaac cttcgataat actattatgg gaaatgttgc tcctattcaa
     8821 agccaagaac aagattcaaa tggtatggtg aaatcaattg tttctcaaac acctggtgcg
     8881 atttcatacc tcgcttttgc atacgttgat aaatctgtcg gaacattaaa actaaatggt
     8941 tttgcgccaa cagctaaaaa tgttactacc gacaactgga aactttggtc atatgaacat
     9001 atgtatacaa aaggtaatga aacaggtcta acaaaagaat tcttagacta catgaaaagt
     9061 gataaagttc aaagtagtat cgtacaacat atgggatata tctctattaa cgacatgaaa
     9121 gttgttaaag atgctgaagg taaagtaacc ccaaaataaa acttaatcac atctagataa
     9181 agcatcttga tcctaggagg aattatgaaa aatcaagaat tagctaaaaa attaacatct
     9241 ccttcaaaaa actctcgatt agaaaaattt ggtaaaggaa taacttttct ttcgcttgcc
     9301 ctaatcgtct tcattgttgc gatgattttg atttttgttg ctcaaaaagg actatctaca
     9361 ttttttgttg atggcgttaa attaacagac ttcttattta atacaaaatg ggaaccaagt
     9421 gctaagtcat ttggtgcttt tccaatgata gctggatcat ttattgttac tattctatca
     9481 gctatcattg ctacaccatt tgctatcggt gctgccgtat ttatgactga aatctcacct
     9541 aaatatggtt ctaaaatttt acaacctgca gttgaattac tagttgggat tccttctgtt
     9601 gtttacggtt ttattggttt acagattatt gttcccttcg ttcgctctat ttttggggga
     9661 acaggttttg gcattttatc tggggtctgt gtcctcttcg tcatgattct accaactgtt
     9721 actttcatga cagtagatag ccttagagct gtgccacgtc actataaaga agcaagttta
     9781 gccatggggg caactcgttg gcaaacaatt tggcgtgtta tattaaacgc tgcacgtcca
     9841 ggaattttca ctgcaattgt ttttggtatg gcacgtgcat ttggtgaagc tctagcaatt
     9901 cagatggttg tcggaaactc tgcaatttta ccaacgtcat tgacaacacc agcagcaaca
     9961 ttaacgtctg ttctaacaat gggtattggt aatactgtaa tgggaactgt acaaaacaat
    10021 gtcctctggt cgctagcctt agtactttta attatgagtt tagcatttaa tacagttatt
    10081 aagttaatta caagggaagg aaagaaaaac tatgaacgct aaaaaggctg acaaattagc
    10141 aactacaata ctctatagta ttgcagctat catcgttaca atcctcgcct ctcttttaat
    10201 ttttattctt gttcgcggat tgccacacgt atcttggtca tttttaactg ggaaatcatc
    10261 atcatatgaa gcaggaggcg gtattggaat acaattatac aattcctttt tcctacttat
    10321 tgttactctg attatttcta ttcctctatc tttaggagct ggaatatacc tctcagaata
    10381 tgctaaaaaa ggacgtttaa caaactttgt tagaacatgt atcgaaattt tgtcatctct
    10441 tccttctgtt gtagttggtc tgtttggtta cctaatcttc gttgttcaat ttcaatatgg
    10501 cttctcaatt atctcaggtg ccttagcact tactgttttt aacttacccc aaatgacacg
    10561 tagtgttgaa gatagccttc aaaatgtaca ccacacacag cgtgaagcag gattggccct
    10621 aggtatttct cgttgggaaa cggtcattta cgtcgttgtc cctgaagctc tgccaagtat
    10681 tgtaactggt gttgtgctag cttcaggacg tattttcggt gaggcagcag cacttattta
    10741 tacagctggt caatctgctc cagcacttga ttggtcaaat tggaacgtcc ttagtgttac
    10801 aagtccaatc tcaattttcc gtcaagcaga aactttagct gttcatattt ggaaagttaa
    10861 tagcgaaggg acaatccctg atgcaacaca agtatctgcc ggtagcgccg cagtcttatt
    10921 ggttgttatt cttattttca acctttcagc tcgctcgatc ggtaagaaac ttcattcgaa
    10981 attaacctcg tctaattaaa aggagtagta atggcagaat ataattggga cgaacgccat
    11041 atcattacct tccctgaaga aaattcagct ctcaccacaa aagatttaca cgtttactac
    11101 ggtgagaaag aagctatcaa gggaattgat atgcaatttg aaaaaaataa aattacagcc
    11161 ttaatcggcc catctggatg tggaaaatca acatatcttc gtagcttaaa tcgtatgaat
    11221 gatacgattg atattgcacg cgtgactgga caaatcatgt atgaaggaat tgatgttaac
    11281 gctcaagata tcaatgttta cgagatgcga aaacatatcg gaatggtttt ccaacgtcca
    11341 aacccgtttg caaaatcaat ttataaaaac attacctttg catatgagcg tgctggtgta
    11401 aaagataaaa aattcttaga tgaagttgtc gaaacctcac taaaacaagc tgctctatgg
    11461 gatcaagtaa aagatgacct tcataaatca gcattcactt tatctggcgg tcaacagcag
    11521 cgattatgta ttgcacgtgc tatcgcagtt aaaccagaaa ttctcttaat ggatgaacct
    11581 gcttcagcac tcgatccgat tgcaactatg caactagaag agacaatgtt tgaactcaag
    11641 aaaaattaca ctataattat cgtaacacat aatatgcaac aagcagcacg tgctagtgat
    11701 tatactgcat ttttctatct tggagactta atcgaatacg ataaaacgaa taacattttc
    11761 caaaatgcta aatgtcaatc tactagcgat tatgtttctg gtcgatttgg atagtgaggt
    11821 aaaagatgac acaacctatt ttacaagtca gtgatttgtc tgtttactat aataagaaaa
    11881 aagctttaaa agaagtctca atggacttct atcctaatga aattacagcc ttaattggtc
    11941 cgtctggttc tggaaagtca acactcttac gagctattaa ccgtatggga gacttaaatc
    12001 ctgaagtcac tctaacagga gcagtgatgt ataacggaca taatgtttat agcccgcgca
    12061 ctgataccgt agaacttcgt aaagaaattg ggatggtttt tcagcagcct aatccatttc
    12121 ctatgtcagt atttgaaaat gttgtttatg gtcttagact caaaggaata aaagacaaag
    12181 ctacgttaga tgaagccgta gagacttcat tgaagggcgc gtctatctgg gacgaggtca
    12241 aagatagatt acacgactct gctcttggtt tatctggtgg tcaacaacaa cgtgtttgta
    12301 ttgccagaac ccttgctacc aagcctaaaa ttatccttct agatgagcca acctctgcac
    12361 ttgacccaat ttctgctggt aaaattgaag agactttaca tggtctaaaa gatcaatata
    12421 ccatgttact tgttacacgt tctatgcagc aagcctcacg tatttctgat cgtacaggtt
    12481 tcttcttaga cggtaactta attgaatatg gcaatacaaa agaaatgttt atgaatccta
    12541 aacataaaga aactgaagat tacattacag gtaaattcgg ttaataccat ataataagat
    12601 cagaaagaga atacaatatg ttaagatcaa aatttgatga agaattagat aaactgcata
    12661 accaattcta tgcaatggga attgaagcta tcggacaaat caaaaaaaca gttcgtgctt
    12721 ttgtcagtca tgatcgagag ttagctaaag aagtgataga agatgatgta acgcttaaca
    12781 actttgaaac gaagttagag aaaaaatcat tggaaattat tgcactccag cagcctgtat
    12841 cacaagactt acgcaccgtt attaccgttt taaaagctac tagtgatgtt gagcgtatgg
    12901 gagaccatgc agcagcagtt gcaaaagcaa ccatccgtat gaagggcgag gaaagaatcc
    12961 cagcagtaga attagaaatc aataatatgg gaaaagcagt taaaaacatg cttgaagaag
    13021 ccttaacagc ttatatcaat ggtgatgatg aaaaagctta cgaagtagca gcaatggatg
    13081 aaatagttga tgattacttc cgtgatatcc aaaaaatggt agttgaaaca attcaaaagc
    13141 atcctgacgt agcatttgcc gccaaagaat acttccaagt attgatgcac ttggagcgaa
    13201 ttggagatta tggcaaaaat atctgtgaat ggattgttta tttaaaaact ggaaaaatta
    13261 tcgagttatg atataattat tgcttgtaga gtggtaattg taagtatagt tcgtgcgata
    13321 gaaaagctgt aactatcttg ttacagcttt tctatctttt ctagcccata caggactagt
    13381 aatgtttcaa atgtttctga attagttagg agttatatga aaactgtaga acattttgtt
    13441 accaaatttg tccctgaaaa ctataacctt ttcttggata ttaatcgtca aactaaaacc
    13501 ttttcaggta acgttgctgt gtctggcgaa gccttagata acaatatctc ttttcaccaa
    13561 aaagggttaa ccattaaatc tgtcttatta gataatcaac cattagattt ccaactagat
    13621 gaagacaatg aagctatgca tattcaattg catgaaactg gtagcatggt attagtcttt
    13681 gaattcagcg gtcacattac cgataatatg acaggtatgt atccatctta ctatacagtt
    13741 aatggcatca aaaaagaggt tatctccaca caatttgaaa gtcattttgc tcgtgaagta
    13801 ttcccaagta ttgacgaacc agaagcaaaa gcaacctttg atttgagcct aaaattcgat
    13861 caaaaagaag gagagattgc tctttcaaat atgccggaaa tcaatgctga acaacgtcaa
    13921 gaaacaggtt tatggacatt tgacaccaca cctaaaatgt cctcatattt attagctttt
    13981 gccttggggg aattgcatgg taaaacaaca cataccaaaa acggcactct tgttggctca
    14041 tatgccacaa aagcccatca gttaaatgaa ttagattttt ctttagatat cgttgtgcgt
    14101 gttattgagt tttatgagga ctactttggt gtacgttatc ctattcctca gtcactccac
    14161 gttgctctac ctgatttttc tgctggagct atggaaaatt ggggattagt aacatatcgt
    14221 gaagtctatc ttttagtaga tgagaactct agtgtttcca gccgtcagca agtagcatta
    14281 gttgtagcac atgaaatagc tcaccagtgg tttggtaacc tcgttactat gaaatggtgg
    14341 gatgatttat ggttaaatga atcatttgct aatatgatgg aatatgtatc aattgattat
    14401 attgaaccaa aacttaatat atttgaagac tttcaaacag gtggtctacc attagctctt
    14461 aagcgtgatg ccacagatgg cgtccaatct gttcacgttg aggtcaatca tcctgatgaa
    14521 attaacacac tttttgatcc agctatcgtt tatgcaaaag gtagccgcct catgcacatg
    14581 ttacgtcgct ggttaggcga tacagatttt gccgcaggac taaaaattta ttttgaaaaa
    14641 catcaatatc aaaatactat tggtcgtgac ctttggaatg cactttctca aacctcagga
    14701 aaagatgttg cagctttcat ggattcttgg ttagagcaac caggataccc tgtgatggca
    14761 gccaaaatag aagaagatga gctcatttta actcaaaaac aattctttat tggtgaacac
    14821 gaagataagt ctcgcttgtg gcaaattcct cttaatagta attgggaagg aatcccagag
    14881 atattaaccg aggaaacggt agtaatcccc aattttagtc agttagctga aaagaacaaa
    14941 gaaaatggtg cactgcgttt caatactgaa aatactgcac attacattac taactatcaa
    15001 ggtcaactca tagagcatat catttctgat cttcctctta tggataacat ttctaaacta
    15061 caaattgtac aagaacgtca tttacttgct gaaagtggta tgatttcata tagtagttta
    15121 atacctttag tctctctttt gagccaagag acatcatatc ttgttaactc tgctattaag
    15181 agtgttattg atggtttatc cttattcgtc caggaagata gccaagatga attcgacttt
    15241 aaagaatttg ttaataaatt atcagcattt aattttaata gacttggctt tgaaaaaaga
    15301 gaaggagaag gagatgatag cgaaatggtc cgtcatctat ccctaagcct tgctctctac
    15361 tcagataatg aacatgctat tgaagaagct caccatattt ttaaagccca cgaaaataat
    15421 atcgctgcta taccagctgc aattcgcctg ttagttctta ccaatgaaat gaagcatttc
    15481 gaatccaaag aacttagcca cttgttatta gagacttata gtactactac cgatgggaat
    15541 ttcaaacgac aactagcgag tgctttatct catactacag atagcaagac actcaagaaa
    15601 ttattgtcag attggaaaaa taaagatatt gtgaaaccac aagacttagc tatgagttgg
    15661 tatgctactt tccttaaaaa ttcttttaca caagaaagtg tctgggagtg ggcccaagaa
    15721 aactgggaat ggatcaaagc aactctaggt ggtgatatga gttttgataa atttgtgatt
    15781 tatccctcaa gtagtttcaa aacagaagaa cgccttgaac aatataaaaa ctttttcgag
    15841 cctcaattaa gcgatatggc gattagccgt aatatttcaa tgggaattaa agaaatttct
    15901 gctcgtgtcc ttttaatcac gaagcaaaaa gaagaagtta tcaatactat caaaaaatat
    15961 atctaataaa ctcaaataga gattgatgga ttattcaatc tctatttctt tgtctgtgtc
    16021 atgaaattag ttgacataaa acatacttta tttaggattt attaagcaaa gtttggtatt
    16081 ctaatattaa tagttcagat aaaatatgta catcgaaagg atatactatg ataaaaattt
    16141 tattaattga agatgacttg agtctttcca attccgtttt tgatttcctt gatgactttg
    16201 ccgatgtcat gcaaattttt gatggagaag agggtcttta cgaagccgaa agtggagtct
    16261 atgatctgat tcttctagat ctcatgttac ctgaaaaaaa cggcttccaa gtgttgaaag
    16321 agctacgtga aaaaggtatt acaacccctg tattaataat gaccgctaaa gaaagcattg
    16381 atgataaggg acaaggtttc gacttaggtg ctgatgatta tctaacaaaa cctttttacc
    16441 tagaagaact aaaaatgcga attcaggctc ttttaaagcg ttctggaaaa tttaatgata
    16501 acagcctaat ttatggtgat attcgcgtag acatgtctac taactcaacc tttgttaatc
    16561 aaacagaagt tgagctctta ggtaaagagt ttgatctttt agtctatttc cttcaaaatc
    16621 aaaatgttat cttaccgaaa tctcaaatct ttgatcgtat ttggggattt gatagtgata
    16681 caacgatttc agttgtagaa gtctatgtct ctaaggttcg gaaaaaatta aaaggaacac
    16741 ttttttcaga aaaccttcaa actttgcgta gtgtaggata tattttaaag catgtcgaaa
    16801 ccgattaaga taagtaaatt caaaaaaaat gtctcagata gtcattttat ccattttttc
    16861 acagttttta gtggtatttt tctggttatg actgttatca tcctccaagt catgcgctat
    16921 ggtgtttatt cttctgttga tagtagttta aagtatatca gtacccatcc taaaaattac
    16981 atcaatatgg ttatgtcacg tactgctgcc tacctagata actccaatat cgcaagtgtc
    17041 aagttaaaac ccggcggtca gacagtagct aatactgata ttattctttt tacatcagaa
    17101 gaagaagtca ttaactattt tgacgcattt tcaaactatc agtttttaaa acctaataaa
    17161 aaaaatctag gtggcattag tgaattaact ttaaccaaca tctttggtca agatgagaca
    17221 tatcatgctg tcaccgtaaa agttaataac cctgcttatc ctaatgtgac ttacatgaca
    17281 gctattgtta atattgatca attagtaaat gctaaagaac gctatgaaaa aattattata
    17341 tttgtcatga ctactttttg gattatttca attggagcaa gtatctactt agctaaatgg
    17401 gcacaaaaac cgattataga aaactacgaa agacagaagg cttttgtcga aaatgctagc
    17461 cacgagctgc gtactcccct agctgtctta caaaatcgcc tagaaacgct attcagaaaa
    17521 ccgaatgcca ccattttaga aaatagtgaa aatatcgctt caagtctaga tgaagtacga
    17581 aatatgcgta ttttaaccac taatctttta aacttagcaa gacgtgatga tggaatcaaa
    17641 ccagaattag ctgttattaa gcctacactt tttgatagta tctttgagaa ttatgatcta
    17701 atcacccaag agaatggtaa aaattttact ggacataata tgatccaaga tagtttcaaa
    17761 acagataaga cactgctaaa acaactcatg actattttat ttgataatgc tataaaatat
    17821 actgacaatg atggtagcat tgactttacc atatctgaaa ctgataagta tctcttttta
    17881 gaaatcgctg ataatggacc aggaatttca gaggaggata aagtcagaat ttttgatcgt
    17941 ttttaccgtg ttgacaaagc aagaacacga caacaaggag ggtttggcct cggcctttct
    18001 ttagctcaac aaatcgttaa ctcactgcga ggtaatatta cagtaattga taataaacct
    18061 agaggcagta tttttaagat aaaactacca aaagtaaaag gttaggccat gttagtccta
    18121 accttttaaa ttttaaagtg gtcttgcata cttgatatag caagctgata ggaaatttgg
    18181 tataataaaa gctatggaaa ttgaaaaaac aaatcgtatg aatgcccttt ttgagtttta
    18241 tgcggcatta ttaacagata aacaaatgaa ttatatcgag ttatactacg ccgatgacta
    18301 cagccttgct gaaatagctg aagaatccgg tgttagccga caagctgtct atgataatat
    18361 caaacgaact gaaaaaattc ttgaggcgta cgaaatgaaa ctacatatgt attcagatta
    18421 tattgtccgt agtcagattt ttgatgacat tctagaaaaa tataccgatg atgccttttt
    18481 acaagaaaaa atttccattc taagtagcat tgataataga gattaaatga ggagaaataa
    18541 tttatggctt tcgaaagttt aacagagcgc ctgcaaggag tttttaaaaa tattcgtggt
    18601 aagaaaaagt tatctgaaaa agatgtgcaa gaagtcacca aggagattcg tctagctctt
    18661 ttagaagctg acgttgctct tccagttgtt aaaaccttta ttaaacatgt tcgtgaacgt
    18721 gctgttggac atgaaatcat tgatacacta gatcctacgc aacaaattgt taagattgtc
    18781 aatgaagagc taacagattt attaggggct gagacctctg aaattgaaaa atcccctaaa
    18841 attccaacca tcattatgat ggttggtcta caaggtgctg gtaaaacaac ttttgccggt
    18901 aagttagcaa acaaactaat caaagaagac aatgctcgtc ctatgatgat agcagctgat
    18961 atttatcgac cagctgctat tgatcagtta aaaacccttg gtagccagat taatgttcct
    19021 gtctttgata tgggaactaa ccattcagca gttgagattg tcactaaagg tttagaacag
    19081 gctcgtgaaa atcgtaatga ctatgttctt attgatacag ccggacgcct acaaattgat
    19141 gcaaccctta tgcaggaatt gcatgatgtc aaagccatag ctcaacctaa tgaaatactc
    19201 cttgtcgttg acagcatgat tggacaggaa gctgcaaatg tcgctgaaga atttaaccgt
    19261 cagctcagca ttagtggtgt tgttctgaca aagattgacg gtgatacacg cggtggtgct
    19321 gccctttcag tacgcgaaat tactggaaaa ccaattaaat ttacagggac aggggaaaaa
    19381 atcactgata ttgaaacttt ccatcccgat cgtatggcaa gccgtatatt ggggatggga
    19441 gacctgctaa cacttattga gcgcgctagt caagaatacg acgaaaaacg ttcaatggaa
    19501 ttagcggaga aaatgcgtga aaacaccttt gacttcaacg attttattga tcaattagat
    19561 caagttcaaa atatggggcc aatggaagat ctccttaaaa tgcttccagg aatggctaac
    19621 aatccagcaa tgaaaaactt caaagtggat gaaaacgaaa ttgctcgcaa aagagccatt
    19681 gtgtcatcaa tgactccaga agagagagaa aatccagatc tcctcaatcc aagtcgtaga
    19741 cgtcgtattg ctgctggatc aggtaatact ttcgttgatg ttaataaatt cattaaagat
    19801 tttaaccagg caaaacaaat gatgcaaggc gtgatgtccg gtgatatgaa taaaatgatg
    19861 aaaaaaatgg gaattgatcc taataacctc cctaaagata tgcctggaat ggacggaatg
    19921 gatatgtcta atttagaagg tatgatgggg caaaatggca tgcctgactt atctagttta
    19981 ggtggcgata tggacttcag ccaaatgttt ggtggtggct taaaaggaaa agtaggagcc
    20041 tttgcagcta aacaatcaat gaaacgtatg gctaataaaa tgaagaaagc taagaagaaa
    20101 cgcaaataaa aatgtctatt taaatagaca tttttatttt tgtctgctat aatagacaaa
    20161 aaggtggtga tatttatgta tttagcatta atcggtgata tcattaattc aaaacagata
    20221 cttgaacgtg aaactttcca acagtctttt cagcaactaa tgaccgaact atctgatgta
    20281 tatggtgaag agctgatttc tccattcact attacagctg gtgatgaatt tcaagcttta
    20341 ttgaaaccat caaaaaaggt atttcaaatt attgaccata ttcaactagc tctaaaacct
    20401 gttaatgtaa ggttcggcct cggtacagga aacattataa catccatcaa ttcaaatgaa
    20461 agtatcggtg ctgatggtcc tgcctactgg catgctcgct cagctattaa tcatatacat
    20521 gataaaaatg attatggaac agttcaagta gctatttgcc ttgatgatga agaccaaaac
    20581 cttgaattaa cactaaatag tctcatttca gctggtgatt ttatcaagtc aaaatggact
    20641 acaaaccatt ttcaaatgct tgagcactta atacttcaag ataattatca agaacaattt
    20701 caacatcaaa agttagccca actggaaaat attgaaccta gtgcgctgac taaacgcctt
    20761 aaagcaagcg gtctgaagat ttacttaaga acgagaacac aggcagccga tctattagtt
    20821 aaaagttgca ctcaaactaa agggggaagc tatgatttct gattttttaa gggataatcc
    20881 aatcctaact ttattatttt gtgcacattt tttagctgac tttcagtggc aaagtcaatc
    20941 cttagcagat agtaaatcac atagttggag gggactctgg agacatctct tgattgtttt
    21001 cctcccccta gcagctttaa tgatattgat accagaaact actttgttaa atttaagcat
    21061 atggggaagt catattgtta ttgatagtat aaagaaattg agttaccctt gggtagagga
    21121 agggcatttc caaaaagcag cttttattat agaccaacta gcccactata cttgcattat
    21181 cgttttttac catgcgcttc ctacatattt accgcctaat cattggcttt tgcctatcaa
    21241 gcactttatt gtcatagctc tggtttttat cattatcacc aaacccatta atattgtgtt
    21301 taaaattttc tttaataaat ttcaagctaa agaattatca tcattattaa cacaagaaaa
    21361 aacaaaaata atgaaagaaa aatctgaaga tcatgaagaa acaattgaag gagcaggagc
    21421 tatgattggt aatttagagc gtctaattat ggctatatta ctaatctcag gacagtatgc
    21481 tgctataggt cttgttttta ctgcaaaatc cattgcacgt tacgataaaa tttctaaaag
    21541 tcaggttttt gccgaatatt atttaattgg atcattgttc tcaattataa gtgttttgat
    21601 aactcattgg ttacttttgg tatagaaaag cgctagaaat atcctaacgc ttttctttta
    21661 ttctgtctta taagcataag taaaatcaac attcaaacca gttgaatttc ttaccaaccc
    21721 ctttaattta gaattttgga gtcctttttt ccctaggtaa taaactgggt tataatatga
    21781 ttgatcaaat aaagcttttt cagcagtttt gtaatcatta gcggcatctc ctggtttcaa
    21841 ggcgtcagta gtaattgcct tttgataggc attgtcatac tctttagaag aaaatttacc
    21901 ataattatac gcagaattag tcgtgaataa cccatagaaa gttgatcctt ctggataatc
    21961 tcctccccat gagaagagca cgacatcaaa attttcattt tttgcatctt caaggcgttg
    22021 cttgaaagta acaaattttt cttcaacagt tagaccaggc aaagcactct cccatgtaga
    22081 tttcacatag tcaacagcat ttttagctgc tggagagtcc gaatcagctg tgattgttaa
    22141 cttgagagat gactgaccaa cctcagcaag accttcttta aaaagttttt gagctttact
    22201 tgtattatac gtataacctg gggcagtata cttggaaaga tcctcaccat taggtaattt
    22261 ggctaatttt ttagggacca gagattcagc aggagttgaa cctgtatcaa ctgccgcttt
    22321 aacaacacct ttacgatcag tcgctaaatt caaagcttgc ctaattttct gatttgtcaa
    22381 tgctttaacc gaaccagttt ggttataaat aatatatgta gtacgtgcat cagacgcatc
    22441 tactacatct ttattatttt tattagcttg ataaatagca gaagttcctg atatttcagc
    22501 aaaatcaatc tgaccttgct tgtacatttg aactgcagta tctggttttt tgatagtttg
    22561 aactatgaca ctatttgttt taacatgttt agaatcccaa taatatttat ttttgactaa
    22621 tttaaattta ccatttgaac cattccaatc cttaacaagg taaggacctg agtaaatttg
    22681 attttttgat gttgtaccat aatctttacc aactttttca acatacgatt gtttttgagg
    22741 cataaaatta gagaaggcta aataatattt aaattgaggg catggacttg tcaatttaaa
    22801 ggtcacttga ttgcctttag ctgttacacc aagtttattt aaatctttaa tatctccaga
    22861 attaatctta tcagcattta aaaggtgcga ttctgtcgcc aagtaagcat attcagatgc
    22921 agttttagga tcaactattc tccgccatgt gtaaacaaaa tcttcagcgc ttagtttgct
    22981 tccatctgac cattttaaat tatcacgaag tgtagcagta taagtaagac catcagaaga
    23041 aacggatact tttttagcta aatcgggttt tggtttgccc tctttatcaa ttctaagtaa
    23101 attgctgcct gaattaccta tagctaagtt tgaataagca tctgtattct ttgaaatgtc
    23161 caatgtactg atttcagtag gaatatacca attaataact ttattatctc cagagttctt
    23221 tgactcatta ccacaggcaa ccagggtaac tgtggtggct gttagagtcg ccaaagatat
    23281 acccaaaaac ttaagttttt ttctattaaa actagacata ataccagtcc tcctaaacct
    23341 tctataaaga tataggacat attatatcat aaaatttcaa
//
LOCUS       DATRMA010000026        23192 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.026, whole genome shotgun sequence.
ACCESSION   DATRMA010000026 DATRMA010000000
VERSION     DATRMA010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 23192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23192
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.026"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            228..953
                     /locus_tag="VBC28_000970"
     CDS             228..953
                     /locus_tag="VBC28_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6814970.1"
                     /translation="MLKIENVTGGYVNIPVLKNISFEVNDGELVGLIGLNGAGKSTTI
                     NEIIGILRPYQGDITIDGISLEADQELYRKKIGFIPETPSLYEELTLREHLETVAMAY
                     DIATDEVMARAQKLLEMFRLTDKLDWFPMHFSKGMKQKVMIICAFVVSPSLFIVDEPF
                     LGLDPLAISDLINLLAEEKAKGKSILMSTHVLDSAEKMCDRFVILHKGEIRAVGTLEE
                     LRAIFGDSNANLNDIYIALTKEV"
     gene            955..1989
                     /locus_tag="VBC28_000971"
     CDS             955..1989
                     /locus_tag="VBC28_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000732713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6814971.1"
                     /translation="MKKLFNKRRSLFLTQNSKYLRYVFNDHFVLVLMFLSGFLLYQYS
                     QLLKDFPKTHWPIIVIVSIIILMLLAMGGIASYLEPADKQFLLIKEEAIKEIINSAKK
                     RTYIFWLVIQTLFLVLISPILIKLGLSVFMITLLIFGLGIIKWLVITYKVKVFYNNQN
                     LNWDAAINHEQERKQSILKFFSLFTNVKGISISVKRRSFLDGILKLISKTPSRLWTNL
                     FVRAFLRSSDYLGLTIRLVTLNILSVIFVNETYLALALAFVFNYLLLFQLLALGHHFD
                     YQYMNQLYPVRLNAKASQLKGFLRVLSYAVTVIDSILIRELKPVILLIVLMLIVTEYY
                     IPYKIKKMID"
     gene            2047..2847
                     /locus_tag="VBC28_000972"
     CDS             2047..2847
                     /locus_tag="VBC28_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000155919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEO6814972.1"
                     /translation="MTISNQELTLTPLRGKSGKAYIGTYPNGERVFVKYNTTPILPAL
                     AKEQIAPQLLWARRTSNGDMMSAQEWLDGRTLTKEDMGSKQIIHILLRLHKSRPLVNQ
                     LLQLGYKIENPYDLLMDWEKQTPIQIRENTYLQSIVTELKRSLPEFRTEVATIVHGDI
                     KHSNWVITTSGLIYLVDWDSVRLTDRMYDVAYILSHYIPQKHWKDWLSYYGYKDNEKV
                     WSKIIWYGQFSYLSQIIKCFDKRDMEHVNQEIYELRKFRELIKKHNAS"
     gene            2837..3472
                     /gene="trmB"
                     /locus_tag="VBC28_000973"
     CDS             2837..3472
                     /gene="trmB"
                     /locus_tag="VBC28_000973"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEO6814973.1"
                     /translation="MRVRKRKGAEEHLENNPHYVISNPEEAKGRWHEIFGNNNPIHIE
                     VGSGKGAFITGMAEQNPDINYIGIDIQLSVLSYALDKVLDSGAKNIKLLLVDGSSLSN
                     YFDTGEVDLMYLNFSDPWPKKKHEKRRLTYKTFLDTYKDILPEQGEIHFKTDNRGLFE
                     YSLASFSQYGMTLKQVWLDLHASDYQQNIMTEYERKFSNKGQVIYRVEARF"
     gene            3516..3603
                     /locus_tag="VBC28_000974"
     tRNA            3516..3603
                     /locus_tag="VBC28_000974"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3550..3552,aa:Ser,seq:gga)
     gene            3962..4438
                     /gene="rimP"
                     /locus_tag="VBC28_000975"
     CDS             3962..4438
                     /gene="rimP"
                     /locus_tag="VBC28_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018366632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEO6814974.1"
                     /translation="MANQTIVDIVTQVVTPAIQAPFELVDVEYEKMGGDYVLSILIDK
                     PGGITVEDTAQLTDVVSPLLDTIQPDPFPEQYMLEVSSPGLERPLKTAEALSNAVGSY
                     INVSLYKSIDKVKIFEGDLLSFDGETLTIDYMDKTRHKTVDIPYQTVAKARLAVKL"
     gene            4465..5625
                     /gene="nusA"
                     /locus_tag="VBC28_000976"
     CDS             4465..5625
                     /gene="nusA"
                     /locus_tag="VBC28_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HEO6814975.1"
                     /translation="MDTMSKEMLEAFRILEEEKHINKEDIIDAVTESLKSAYKRRYGQ
                     SESCVIEFNEKKADFTVYTVREVVDEVFDSRLEISLKDALAISSAYELGDKIRFEESV
                     TEFGRVAAQSAKQTIMEKMRRQMREVTFNEYKQHEGEIMTGTVERFDQRFIYVNLGSL
                     EAQLSHQDQIPGESFKSHDMIDVYVYKVENNPKGVNVFVSRSHPEFIKRIMEREIPEV
                     FDGTVEIMSVSREAGDRTKVAVRSHNSNVDAIGTIVGRGGSNIKKVISNFHPKRVDAK
                     TGLEIPVEENIDVIQWVEDPAEFIYNAIAPAEVDMVLFDDEDTKRATVVVPDSKLSLA
                     IGRRGQNVRLAAHLTGYRIDIKSASEYEKMEAQELQTEEVAQESEVISDKAE"
     gene            5647..5943
                     /locus_tag="VBC28_000977"
     CDS             5647..5943
                     /locus_tag="VBC28_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006531117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HEO6814976.1"
                     /translation="MAKTKKIPLRKSVVSGEVIDKRDLLRIVKNKEGQVFIDPTGKQN
                     GRGAYIKLDNDEAILAKKKRVFDRSFSMEVSDEFYDELLAYVDHKVKRRELGLE"
     gene            5936..6238
                     /locus_tag="VBC28_000978"
     CDS             5936..6238
                     /locus_tag="VBC28_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HEO6814977.1"
                     /translation="MNNSEKVLNLIGLAQRAGRLITGEELVIKAIQNQQVSLIFLAND
                     AGPNLTKKVTDKSNYYKTEVSTVFSTLELSDALGKPRKVVAVADAGFSKKMRTLMA"
     gene            6258..9041
                     /gene="infB"
                     /locus_tag="VBC28_000979"
     CDS             6258..9041
                     /gene="infB"
                     /locus_tag="VBC28_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017766792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEO6814978.1"
                     /translation="MSKKRLHEIAKEIGKTSKEVVEQAQSLGLPVKSHASSVEENDAT
                     RIVESFSSSKTKAPTNSVQTNQGVKTESKTVETKQGLSDDKPSTQPVAKPKPQSRNFK
                     AEREARAKAEAEKRQHNGDHRKNNRHNDTRSDDRRHQGQKRSNGNRNDNRQGQQNNRN
                     KNDGRYADHKQKPQTRPQQPAGNRIDFKARAAALKAEQNAEYSRHSEQRFREEQEAKR
                     QAAKEQELAKAAALKAQEEAQKAKEKLASKPVAKVKEIVNKVAATPSQTADSRRKKQT
                     RSDKSRQFSNENEDGQKQTKNKKNWNNQNQVRNQRNSNWNHNKKNKKGKTNGAPKPVT
                     ERKFHELPKEFEYTEGMTVAEIAKRIKREPAEIVKKLFMMGVMATQNQSLDGDTIELL
                     MVDYGIEAHAKVEVDEADIERFFADEDYLNPDNLTERPPVVTIMGHVDHGKTTLLDTL
                     RNSRVATGEAGGITQHIGAYQIEEAGKKITFLDTPGHAAFTSMRARGASVTDITILIV
                     AADDGVMPQTVEAINHSKAAGVPIIVAINKIDKPGANPERVISELAEHGVISTAWGGE
                     SEFVEISAKFGKNIQELLETVLLVAEMEELKADADVRAIGTVIEARLDKGKGAVATLL
                     VQQGTLNVQDPIVVGNTFGRVRAMTNDLGRRVKVAGPSTPVSITGLNEAPMAGDHFAV
                     YADEKAARAAGEERAKRALLKQRQNTQRVSLENLFDTLKAGEVKSVNVIIKADVQGSV
                     EALAASLLKIDVEGVKVNVVHSAVGAINESDVTLAEASNAVIIGFNVRPTPQARQQAD
                     ADDVEIRQHSIIYKVIEEVEEAMKGKLDPEYQEKILGEAIIRETFKVSKVGTIGGFMV
                     INGKVTRDSSVRVIRDGVVIFDGKLASLKHYKDDVKEVGNAQEGGLMIENYNDLKEDD
                     TIEAYIMEEIKRK"
     gene            9132..9500
                     /gene="rbfA"
                     /locus_tag="VBC28_000980"
     CDS             9132..9500
                     /gene="rbfA"
                     /locus_tag="VBC28_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEO6814979.1"
                     /translation="MKKGLIMANHRIDRVGMEIKREVNEILRLRVNDPRVQDVTITDV
                     QMLGDLSMAKVFYTIHSTLASDNQKAQIGLEKATGTIKRELGKNLTMYKIPDLQFVKD
                     ESIEYGNKIDEMLRNLDKKD"
     gene            complement(9584..10588)
                     /locus_tag="VBC28_000981"
     CDS             complement(9584..10588)
                     /locus_tag="VBC28_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEO6814980.1"
                     /translation="MKPSFKKLLLLFSIITILSIACTPHAKASGRSWKSWFIEQYFWL
                     KRDKSYYKVQDESSFQKYLNASREQSDKGYYLDPNSVNGGLVQERLFDMQVYSWNDNG
                     KANQKTIFYLAGGSYLNNPTPYHISMLKTLSTSLDAKIILPIYPKTPRYTYDYAIPRL
                     VNLYRHFHEKNANLTLMGDSAGGGLALGLAHALSHQSGQEAIPQPKNIILLSPWLDVT
                     MKHPEIPKYEDTDPILSAWGLARVGEIWANGSNNTNHTYVSPKNAPATKLAPITLFTG
                     TREIFFPDIRDYAAQLQAANHPVNYIAQEGMNHVYPIYPIEEAKTAQYQMIDIINKTP
                     "
     gene            10752..11168
                     /locus_tag="VBC28_000982"
     CDS             10752..11168
                     /locus_tag="VBC28_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HEO6814981.1"
                     /translation="MTISSAEWEIMRVVWAQQNTTSNEILAVLLEKYDWTPSTVKTLL
                     RRLLDKGYVSREKMGKGFSYSPLIDEDLAMMSEVDSVFQKVCQTKHVAIVRHLLESIP
                     MTEKDRLNLQSSLEAKKGKTLERVACNCIPGQCQCH"
     gene            11181..13415
                     /locus_tag="VBC28_000983"
     CDS             11181..13415
                     /locus_tag="VBC28_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HEO6814982.1"
                     /translation="MANRKETFLIDGMTCASCALTIEKAVNKLDHVDSAVVNLATEKM
                     TVTFDDTTLSPNVIEECVSESGYEASLFKEETSKSQSERHQLAIEKMWHRFWMSAVAT
                     IPLLYISMGPMINLWLPSFLMPDKGPLNYGMIQLLLTLPVMYFGRIFYQNGFKALFKR
                     HPNMDSLVAIATTAAFIYSLYGLYEILQGDIHYAHQLYFESVAVILTLITLGKYFEIL
                     SKGRTSASIEKLLTLSAKEARVIKDGEDYMVPLDKVKIGETILVKPGEKIPLDGHVVA
                     GESSIDESMLTGESIPVEKKVGSKVYGASINGQGSLTIFVEKEAGGSLLSQIINLVEA
                     AQTSKAPIANLADKVSGVFVPFVIVIAILSGLSWYLILGQSFAFSLKIMIAVLVIACP
                     CALGLATPTAIMVASGKAAENGILFKGGEVLEKAHHIDTIVFDKTGTLTKGKPEVVAI
                     KTYGGDKEEFLGQVASVEKLSNHPLSQTIVNKAKEKELPLREVMAFKNILGYGLSATI
                     NGKTMLVGNANLMTKNDVNLDLAKADIEIAQEEAQTVVYVSENGVLSGLITLTDQLKT
                     DSQETVKQLQRLGFNLVLLTGDNKASADAIAQKLGITTVVSEVLPDQKANVILELKEK
                     GGQIAMVGDGINDAPALASSDVGISMSSGTDIAIESADIVLMKPELTDLLKAMTISKQ
                     TIQIIKENLFWAFFYNVLAIPVAMGVLHLFGGPLLNPMLAGLAMAFSSVSVVLNALRL
                     KVLK"
     gene            13456..13662
                     /locus_tag="VBC28_000984"
     CDS             13456..13662
                     /locus_tag="VBC28_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000683378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HEO6814983.1"
                     /translation="MKHTYRVSGMKCDGCAKTVSDKLSSVIGVDEVNVDLTKNQVVVS
                     GKTFKWLLKRSLKDTKYSLEEEIN"
     gene            13772..14386
                     /locus_tag="VBC28_000985"
     CDS             13772..14386
                     /locus_tag="VBC28_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000020717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HEO6814984.1"
                     /translation="MSIDIWNILSLVGTVAFASSGAIVAIEEEFDILGLFILGFVTAF
                     GGGAIRNVLIGLPIETLWSQGIAFYAAAAAILFIMIFPNLLSGKGRDAEVVSDAIGLA
                     AFSVQGALYATQSHQPLSAVIVAAVLTGAGGGIVRDVLAGRKPGVLRSEIYAGWSILV
                     GIILYFKIAKTTTDYYLLVLVVTSLRMLGYKKQWHLPVVRPRKK"
     gene            14401..15213
                     /locus_tag="VBC28_000986"
     CDS             14401..15213
                     /locus_tag="VBC28_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEO6814985.1"
                     /translation="MIKLIAIDMDGTLLNDEKKIPKENIQAIKEATQAGIKIVLCTGR
                     PMSGILPYFNELGLTKEEYIIMNNGCSTYSTKDWQLIDSATLTHDELIFLEEVVKEFP
                     NVCLTLTAENTFYAVGEEVPEIVAYDADLVFTKAKSTSLDALRNQEEIVFQAMYMGLD
                     ADVTAFQEAVEEALISKFSGVRSQDYIYEIMPQGVTKARGLKSLIAKLGLDINQVMAI
                     GDAPNDIELLDLVPNSVAMGNASDEIKSRCKYITVDNNKAGVAKAIYDYALR"
     gene            15326..17968
                     /gene="polA"
                     /locus_tag="VBC28_000987"
     CDS             15326..17968
                     /gene="polA"
                     /locus_tag="VBC28_000987"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEO6814986.1"
                     /translation="MTNKNKLLLIDGSSVAFRAFFALYNQIDRFKNNSGLHTNAIYGF
                     HLMLNHILGRVQPSHILVAFDAGKTTFRTEMYADYKGGRAKTPDEFREQFPYIRQQLD
                     VLGIKHYELEHYEADDIIGTLAKQAEASNEHFDITVVSGDKDLIQLTDTNTVVEISKK
                     GVAEFEEFTPAYLMEKMGITPSQFIDLKALMGDKSDNIPGVTKIGEKTGLKLLSEYGS
                     LEGIYENIEAMKQSKMKENLINDKEQAFLSKTLATINIASPITIGLEDILYSGPQDIK
                     ALSQFYDEMDFKQFKAALGEETSQEDFEVDFTEVEQLKTEMFSDNDFYYFEMLGDNYH
                     VEDLIGIAWGNSDTIYATSNVSLLQEALFKKALSKPIKTYDFKRSKVLLNRFNIDLPE
                     PAFDTRLAKYLLSTTEDNLVSTIARLYTNLPLDTDDAVYGKGAKRAIPEKTRFLEHLA
                     KKVKVLVDSEANIMQQLKANEQEELLFEMEQPLANVLAKMEIRGIKVKKNTLNEMAIE
                     NQKVIETLTQEIYELAGQEFNINSPKQLGKLLFETLGLPVEMTKKTKTGYSTAVDVLE
                     RLAPISPLVTKILEYRQITKLQSTYIIGLQDYILEDGKIHTRYVQDLTQTGRLSSSDP
                     NLQNIPVRLEQGRLIRKAFVPSEDNAVLLSSDYSQIELRVLAHISKDEHLIAAFKEGA
                     DIHTSTAMRVFGIEKPENVTPNDRRNAKAVNFGIVYGISDFGLSHNLGIPRKLAKQYI
                     DTYFERYPGIKNYMETVVREAKDKGYVETLFHRRRSLPDINSRNFNIRQFAERTAINS
                     PIQGSAADILKIAMINLDRVLDKGGYKSKMLLQVHDEIVLEVPNEEIGAIRELVTKTM
                     ESAISLSVPLIADENAGETWYEAK"
     gene            17998..18438
                     /locus_tag="VBC28_000988"
     CDS             17998..18438
                     /locus_tag="VBC28_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEO6814987.1"
                     /translation="MVYHFQNPSDFMLKNYLTKAKTIAVVGLSDRQETAAYQVSKIMQ
                     EAGYQIIPVNPKNAGQKILGQMTYASLKDVTEHIDIVNIFRRSEYLPDIAREFLEVDA
                     DIFWAQLGLESQEAETILKQAGHKQIVMNKCLKVECQKLHSETN"
     gene            18520..18999
                     /gene="perR"
                     /locus_tag="VBC28_000989"
     CDS             18520..18999
                     /gene="perR"
                     /locus_tag="VBC28_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide-responsive transcriptional repressor
                     PerR"
                     /protein_id="HEO6814988.1"
                     /translation="MDSHSHGHRPLDAYENVLEHLREKRIRITETRKAIISYMVNSRE
                     HPSAEKIYNDLLPEYPNMSLATVYNNLKVLVDEGFVTELKLCNYSTTYYDFMGHQHLN
                     IACEDCGKIVDFVDVDLLDISREAHQQTGFEVTRVQLVAYGICPECQRKRKDKYKKD"
     gene            19152..20717
                     /locus_tag="VBC28_000990"
     CDS             19152..20717
                     /locus_tag="VBC28_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000703861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK signal domain/LPXTG anchor domain surface
                     protein"
                     /protein_id="HEO6814989.1"
                     /translation="MKISQYNKWSIRRLKVGAASVMIASGSIVALGQSHIVSADEMSQ
                     PKTTITAPTANTSTNVESSTDKALSKVTTMETSSEMPKMQNMAKVEKTSDKPMMVATS
                     VRKMMATPTPVAMTKTTSVDEVKKSTDTAFKQTVDVPAHYVNAAKGNGPFLAGVNQTI
                     PYEAFGGDGMLTRLILKSSEGAKWSDNGVDKNSPLLPLKGLTKGKYFYQVSLNGNTTG
                     KEGQALLDQIKANDKHSYQATIRVYGAKDGKVDLKNMISQKMVTINIPHITTDMEVKN
                     SLKMAFKEKVDVPAKYVSAAKAKGPFLAGVNETIPYEAFGGDGMLTRLILKASEGAKW
                     SDNGVDKNSPLLPLKDLTKGKYFYQVSLNGNTAGKKGQALLDQIKANGSHTYQATITI
                     YGTKDGKVDMNTILGQKTVMIHINVAKKDMNSTSMMMKKDKMTMPMKKEMTSSKINTG
                     MMMSNNKMSANMQMSSQAKSNDKAGKKMSMMSKNLPNTGETKQQNVGVLGMLSLAFAT
                     GLTALGLKKSKQR"
     gene            20830..21516
                     /locus_tag="VBC28_000991"
     CDS             20830..21516
                     /locus_tag="VBC28_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO6814990.1"
                     /translation="MTQKLLLVDDEFEIIDINRRYLEQAGYEVSVAADGIEALKEVDE
                     NRFDLIISDIMMPKMDGYDFISEVLVREPNQPFLFITAKVSEPDKIYSLSMGADDFIS
                     KPFSPRELVLRVKNILRRIYGNHQQSEVLTIGDLVIDQKQRLVMVDCNTISLTNKSFD
                     LLWILANHLNRVFSKTELYERVWGEEFLDDTNTLNVHIHALRNDLAKFSTDNTPTIKT
                     VWGLGYKLEE"
     gene            21518..22555
                     /locus_tag="VBC28_000992"
     CDS             21518..22555
                     /locus_tag="VBC28_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEO6814991.1"
                     /translation="MKLKYYIVIGYLISMLITVAGVFFGLNHMLIETRGVYYILSVTI
                     IACIVGGIVNLFLLSSVFTSLKKLKQKMKDISQRCFDTKAQICSPQEFKDLETAFNQM
                     SSELESTFKSLNESEREKTMMIAQLSHDIKTPITSIQSTVEGILDGIISEEEVNYYLN
                     TISRQTNRLNHLVEELSFITLETMSDTAEPHKEETIYLDKLLIDILSEFQLVFEKENR
                     QVMIDVAPDVSKLSSQYDKLSRILLNLISNAVKYSDPGSPLTIKAYSNRQDIVIDIID
                     QGYGIKDEDLASIFNRLYRVESSRNMKTGGHGLGLYIARQLAHQLNGDILVESQYQKG
                     SKFSLVLKLQK"
     gene            complement(22569..>23192)
                     /locus_tag="VBC28_000993"
     CDS             complement(22569..>23192)
                     /locus_tag="VBC28_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HEO6814992.1"
                     /translation="YYQYNNMTLLDFFVPLPVYFFYTLFIISVSFVMLDVVKNQKLNV
                     RFSDNTYVFSSHIFWKLLSVLVLKGLILSFFYLLSTFGLLIIISSFRLLLSGNLILAP
                     VLIVVSSLITTKAVIKLVQQYYSYSISTLVFYTQLESGNYEGPSKVLVASRELMNGNK
                     LRLFLLDLSFIGWQFLTIFSFGLVYIYLLPYQTTARLIFYRNITKNS"
BASE COUNT         7923 a         3509 c         4697 g         7063 t
ORIGIN      
        1 aggtgtcttt ttttgtccga tgagtgatgg aggtgctttt tgtaaacata atgggagcat
       61 ggggtagctt ggcatttcat tagatgtaaa agcggacaaa ataggtttaa agttattttc
      121 aaaactataa gggaattcta aagtacaatt atccataaga atagaccttc cctttcttat
      181 tttgttataa tagttaataa agtatatcaa agatagagag aatgacaatg ttaaaaattg
      241 aaaatgttac aggggggtat gttaatatcc cagttttaaa aaatatttct tttgaagtaa
      301 atgatggaga gttagttgga ttaattgggc ttaatggtgc aggaaaatca accactatta
      361 acgagattat tggtattttg agaccctatc aaggagatat tacaattgat ggtattagtc
      421 ttgaagccga ccaagaactt tatagaaaga aaataggctt tattcctgag acaccaagtc
      481 tctatgagga gctaactctc agggaacatt tagaaacggt agctatggcg tatgatattg
      541 ccaccgatga agttatggca cgtgcgcaaa aactattaga gatgtttcgt ctaacagata
      601 aattagactg gtttccaatg catttttcaa agggaatgaa acaaaaggtt atgattattt
      661 gtgcttttgt tgtatctcca agtttattta ttgttgatga accattttta ggtttggatc
      721 cactagctat ttcagatttg attaatcttc tagcagaaga aaaagctaaa gggaaatcta
      781 ttttgatgtc aacacacgta ttagattctg ccgaaaaaat gtgtgatcgt tttgttattt
      841 tacataaagg agaaatacgg gcagttggaa ccctagagga attacgggct atttttggag
      901 acagtaatgc taatttaaat gacatctaca tagctctgac aaaagaggtg tgatatgaaa
      961 aagttattta ataaacgtcg aagtctcttt ttaactcaaa atagtaaata tttgagatac
     1021 gtctttaacg atcattttgt attagtatta atgtttttgt caggtttttt gttatatcag
     1081 tatagccagt tactaaaaga ctttccaaaa acacattggc caattatcgt gattgtgtca
     1141 atcattatct taatgttact agcgatgggg ggaatagcca gctatttaga accggctgat
     1201 aaacaatttt tacttataaa agaagaagca ataaaggaaa ttataaattc tgctaaaaaa
     1261 cggacttata tcttttggtt ggttattcaa accttatttt tagtattaat atcaccaatt
     1321 ttgataaagt taggcttgtc agtttttatg attacattgt taatttttgg tctaggaata
     1381 atcaaatggt tggtaataac ttacaaggta aaggtctttt ataacaatca aaatttgaac
     1441 tgggatgcag ctataaatca tgagcaggaa agaaaacaga gtatattaaa atttttctct
     1501 ttgtttacaa atgttaaagg aatttcgata agtgtaaaac gtcgttcatt tttagatggt
     1561 attttaaaac ttatttctaa aacgccatcg cgtttatgga ctaacttatt tgtgagggca
     1621 tttctaagaa gctcagatta tcttggttta acaataaggt tggtaacttt aaatatctta
     1681 tcagtcatct ttgttaacga aacctacctc gcccttgctc tggcatttgt atttaactac
     1741 ttattattgt tccaactatt agctttaggt caccattttg attatcaata catgaatcag
     1801 ctctatccag ttagattaaa tgctaaagca agtcagctaa aaggttttct cagagtatta
     1861 tcatatgctg tgacagtaat tgatagcata cttataagag aattgaaacc tgttatatta
     1921 ttgatagttt tgatgttaat tgtgacggaa tattacatcc cttacaaaat aaagaagatg
     1981 attgactaaa gcctataaaa caagtaaaat aagaggaaac gactaaatga aaaggaggga
     2041 ctgctggtga caatatcaaa tcaagaattg acgcttactc ccttaagagg aaaaagtggc
     2101 aaagcctata ttggtactta tccaaatggt gaacgtgtct ttgtcaaata taatacgaca
     2161 cctattttgc cagccttagc taaggaacag attgcgccac aactactgtg ggcacgccgt
     2221 acaagtaatg gcgatatgat gagtgctcaa gaatggttag atggtcgaac attgactaaa
     2281 gaagatatgg gtagtaaaca gattattcat attttattgc gcctgcacaa gtctcgtccc
     2341 ttagtaaatc agctgttaca attagggtat aaaattgaaa acccttatga tttgttgatg
     2401 gattgggaaa aacaaactcc tattcaaatc cgggaaaaca cttatttgca atctattgtg
     2461 actgagttaa agcgtagttt acctgagttt cgaacagagg ttgcaacaat cgttcacgga
     2521 gatatcaaac atagcaactg ggttattaca acaagtggtt taatttattt ggttgattgg
     2581 gattctgttc gtttaacaga tcgcatgtat gacgttgcat atattttaag tcattatatt
     2641 ccacaaaaac actggaaaga ttggctgtct tactacggtt ataaagataa tgagaaagtc
     2701 tggagcaaaa ttatctggta tgggcaattt tcatatttat cacagataat caaatgtttt
     2761 gataaacgag atatggagca tgtcaatcaa gaaatttatg aattacgaaa gtttagagag
     2821 ttaattaaaa agcataatgc gagttagaaa acgtaagggt gcagaggaac acttagaaaa
     2881 taatcctcac tatgttattt caaatccaga agaagccaaa gggcgttggc atgagatttt
     2941 tggaaataat aaccccattc acatagaagt tggttccgga aaaggggctt ttatcactgg
     3001 tatggcagag caaaatccag atatcaatta tattggaatt gatattcaat tgtcagtgct
     3061 ttcctatgct ttggataaag ttctagatag cggtgctaag aatatcaaat tactattagt
     3121 tgatggttct agtctatcaa actactttga tactggcgaa gttgatttga tgtatcttaa
     3181 ctttagtgat ccatggccta agaaaaagca tgagaaacgt cgattgacct ataagacttt
     3241 cttggatacc tataaagaca tacttcctga gcagggggaa attcatttta aaacagataa
     3301 tagaggttta tttgaatata gccttgctag cttttctcaa tacggtatga ctttaaaaca
     3361 agtttggtta gatttacatg caagtgatta ccagcaaaat atcatgactg agtacgaacg
     3421 caaattttca aataaaggac aggttatcta tcgtgtagaa gcacgatttt agaataagta
     3481 aatagtaagt gggtagttat ttgctactcc tttatggaga ggtcgcatag tggctgagtg
     3541 cgcacgcttg gaaagcgtgt agtccctaat gggggctcgg gggttcgaat cccctcctct
     3601 cctttaatct tgttaatgta tcagtgttta ttaagtgttc acctttttca ctcgtcaaaa
     3661 gtccaagttt cgaaaaattt cttttatttt atcatcgttt ctctttgagc ttgaagtatt
     3721 agcataaagt tttctaagct cttacaaaga actgaggaca aaattttaat tttttgatat
     3781 aagattagtg tacggtgatg ctaatttaaa gtaaatgatt gcaataatcg tattttgtgc
     3841 tataatagta ttaaataata cagaaagggg cggagtttta cttcgcctct tatgtgtgtg
     3901 attcatagaa tttatagtca ttataagttc taaagcaagt tagaaagaag aggtagctgt
     3961 tatcgcaaat caaactattg ttgatattgt tactcaagtc gtaactcctg ctattcaagc
     4021 accgtttgaa ttagtagatg tagaatacga aaaaatgggt ggcgactatg tgctcagtat
     4081 tttaattgat aaacccggag gcattacggt tgaagataca gcacaattga cagacgttgt
     4141 aagtccctta cttgacacaa ttcaaccaga tccatttcca gaacagtata tgcttgaagt
     4201 ctcaagtcct gggttggaaa gaccacttaa aacagcagaa gcattatcaa atgctgttgg
     4261 gtcttatatc aatgtaagtc tgtataaatc cattgataaa gttaaaatat ttgaaggtga
     4321 cttattatca tttgatggtg aaaccttgac aattgattat atggacaaaa cacgtcataa
     4381 aactgtagat attccctatc agacggttgc aaaagcccgt cttgcggtta aactttaaga
     4441 aaggatgatt cttgcgaagc aagaatggac actatgagca aagaaatgct agaagccttc
     4501 cgtattttgg aagaagaaaa acacattaat aaagaagata ttattgatgc agttactgag
     4561 tcactaaaat ctgcctataa gcgtcgttat ggtcaatcag aatcatgcgt tattgaattt
     4621 aacgagaaga aagcagactt taccgtctat actgttcgtg aagttgttga tgaagttttt
     4681 gatagccgtt tggaaattag cctaaaagat gcccttgcta ttagctcagc gtatgaactt
     4741 ggtgataaga ttcgctttga ggaatctgtt acagaatttg gacgcgtggc agctcagtca
     4801 gcaaaacaaa ctattatgga aaaaatgcgt cgtcaaatgc gtgaagtaac cttcaatgaa
     4861 tacaaacaac acgaaggtga aattatgact ggtactgttg aacgatttga ccaacgtttt
     4921 atttatgtca atctcggttc acttgaggcg caattatcgc atcaagatca gattcctgga
     4981 gagagcttta aatctcatga tatgattgat gtttatgtct ataaagtgga gaataatcct
     5041 aaaggagtca acgtttttgt tagccgtagt catccagagt ttatcaaacg cattatggag
     5101 cgagaaattc cagaagtatt tgatggaact gttgagatta tgagcgtctc tcgtgaggct
     5161 ggagatcgta ctaaagttgc tgtacgcagt cataattcaa acgttgatgc gattggaaca
     5221 attgttggac gcggtggaag taacattaaa aaagttatca gtaatttcca tcctaaacgc
     5281 gttgatgcta aaacaggatt ggagatcccg gttgaagaaa acattgatgt tattcaatgg
     5341 gttgaagatc cagcggaatt catttacaat gctattgcac cagccgaagt tgatatggta
     5401 ctttttgatg atgaagatac aaaacgtgct actgtagttg ttccagatag caagttatcg
     5461 ctagctattg gtcgccgagg acaaaatgtg cgtttagcag ctcatttgac tggctatcgt
     5521 attgatatta aatctgcaag tgaatacgaa aaaatggaag cgcaagaact tcaaactgag
     5581 gaagtagctc aagaatctga agttatttca gataaggcag aataatataa atgagagagg
     5641 tgttttatgg ctaaaacaaa aaaaatacct ttaagaaaat cagttgtttc tggtgaagtg
     5701 atcgataagc gagatctttt acgaattgtt aaaaataaag aaggacaggt ttttatagat
     5761 cctaccggaa agcaaaatgg tcgaggtgcc tacataaaat tggacaatga tgaagctatc
     5821 ctagcgaaaa agaaacgagt gtttgatcgc agtttttcga tggaagtttc tgatgaattc
     5881 tatgatgaac tattggcata tgtcgaccat aaggtcaaaa gaagagagtt aggccttgaa
     5941 taattcagaa aaagtattaa atctcattgg gttagcacag cgtgcggggc gccttattac
     6001 aggtgaagaa ttggttatta aagcaattca aaatcaacaa gtatcgctaa tctttctagc
     6061 aaatgatgct ggcccaaatt tgacgaaaaa agtaactgat aaaagtaatt actacaaaac
     6121 agaagtctcc acagtgtttt caacactgga attaagtgat gcgttaggca aaccaagaaa
     6181 agtggttgct gtcgcagacg ctggattttc aaagaaaatg aggactctta tggcataaca
     6241 gaataggagg acatcaattg tcaaagaaaa gattacacga aatcgctaag gaaattggaa
     6301 aaacgagtaa ggaagtggtt gagcaagctc aatcgcttgg cttacctgta aaaagtcatg
     6361 cttctagcgt tgaagaaaat gatgctacac gtattgttga gagttttagc tcttctaaaa
     6421 ctaaagctcc aacaaattct gttcaaacta atcaaggtgt gaaaacagaa tctaaaactg
     6481 ttgaaaccaa acaaggcttg tccgatgata aaccttcaac tcaacctgta gctaaaccga
     6541 aaccacaaag tcgtaacttt aaggcggagc gcgaggctcg tgctaaagct gaagcggaga
     6601 agcgtcaaca taatggggat caccgtaaga ataatcgcca taatgataca cgctctgacg
     6661 acagacgcca tcaaggtcaa aagcgttcaa atggtaatcg taatgataac cgacaaggtc
     6721 agcaaaataa ccgtaataaa aatgatgggc gttatgctga tcataaacag aaaccccaaa
     6781 cgcgtcctca gcaacctgca ggcaatcgca ttgactttaa agctcgagca gcagctttga
     6841 aagctgagca aaatgctgaa tactcacgtc atagtgagca gcgtttccgt gaagagcaag
     6901 aagccaaacg tcaagcagct aaggagcaag aactagctaa agcagcagcg ttaaaagcgc
     6961 aagaagaagc tcaaaaagct aaggaaaaac ttgcttctaa acctgttgct aaggtaaaag
     7021 aaattgtcaa taaagtagcc gctacgccat cacagacagc agatagtcgt cgtaaaaaac
     7081 aaactcgttc tgataagtct cgtcaatttt caaatgaaaa tgaagatgga caaaagcaaa
     7141 caaaaaataa gaaaaactgg aataatcaaa accaagtgag aaatcaaaga aatagtaact
     7201 ggaatcataa taagaaaaat aaaaaaggta agactaatgg agctccaaaa ccagtaacag
     7261 agcgtaaatt ccatgaatta ccaaaagaat ttgaatatac tgagggcatg accgttgcgg
     7321 aaattgcaaa acgtattaaa cgcgaaccag cggaaattgt taaaaaactc tttatgatgg
     7381 gagttatggc aacgcagaat caatcattag atggagacac tattgagcta ttgatggttg
     7441 actacgggat tgaagcacat gctaaggttg aagttgatga agctgatatt gaacgcttct
     7501 ttgcagatga ggattacctt aatcctgata acttaacgga acgtccgcct gttgttacta
     7561 tcatgggaca cgttgatcat ggtaaaacaa ctcttcttga tactctacgt aattcacgtg
     7621 tcgctactgg cgaagctggt ggaattactc aacatattgg tgcttatcag attgaagaag
     7681 ctggcaagaa aattactttc ttggatacac cagggcatgc ggcctttact tctatgcgtg
     7741 cgcgtggtgc atcagtaact gatattacta tccttatcgt tgccgctgat gatggtgtta
     7801 tgccgcagac agttgaggca attaatcact ctaaagctgc gggtgtccca attattgttg
     7861 ctatcaacaa aattgataaa cctggtgcaa atccagaacg cgttatcagt gaattagctg
     7921 agcatggtgt catctctact gcatggggtg gtgagtctga atttgttgaa atttcagcta
     7981 agtttggtaa aaatattcaa gaattacttg agacagttct tttagttgct gaaatggaag
     8041 aattaaaagc agatgcagat gttcgtgcta ttggtactgt tattgaagcc cgtcttgata
     8101 aaggtaaggg agccgttgca acgcttcttg tccaacaagg tactttaaat gtacaagatc
     8161 caatcgttgt tggtaataca tttggacgtg ttcgtgcgat gactaatgat cttgggcgtc
     8221 gtgtcaaagt tgcgggtcca tctacaccag tttctattac tggtttgaac gaagctccaa
     8281 tggctggtga tcattttgct gtttatgcag atgagaaagc tgcgcgtgct gcaggtgaag
     8341 aacgtgcaaa acgtgctctc ttaaaacaac gccaaaacac gcaacgtgtt agcctcgaga
     8401 acttatttga tacccttaaa gctggtgaag ttaagtcagt taatgttatt atcaaagctg
     8461 atgtgcaagg ttctgtagaa gcacttgcag cttctctcct taagattgat gttgaaggag
     8521 taaaagttaa cgttgttcac tcagcagttg gtgctattaa tgagtctgat gtaacacttg
     8581 ctgaagcatc aaatgctgtt attataggat ttaacgtacg ccctactcca caagctcgtc
     8641 aacaagcaga tgctgatgat gtcgaaattc gtcaacatag cattatctat aaagttatcg
     8701 aagaagttga agaagcgatg aaaggtaaac ttgatcctga ataccaagaa aaaatccttg
     8761 gtgaagccat tattcgtgag acctttaaag tttcaaaagt gggaactatt ggtggattta
     8821 tggttatcaa tggtaaagtt actcgtgatt caagtgttcg tgttatccgt gatggtgttg
     8881 tcatttttga tggtaaatta gcaagtctta aacactataa agatgatgtt aaagaagtcg
     8941 gcaatgctca agaaggtggt ctaatgattg agaattataa cgatctcaaa gaagatgaca
     9001 ctattgaagc atacattatg gaagaaatta aacgtaaata acccctatga gaaactcaac
     9061 ttgtatcttt agttggtagg gaaatacaaa attatggcta ggctcgtcct agctttcaag
     9121 ttatgagcac aatgaagaaa ggattaatta tggctaatca tcgtattgat cgcgttggaa
     9181 tggaaattaa acgtgaagtc aatgagattt tgcgtctccg agttaatgat ccacgtgtac
     9241 aagatgtcac aattactgat gttcagatgt tgggtgacct ttcaatggct aaagtatttt
     9301 atactattca ttcgacactt gcttctgata atcaaaaagc acaaattggt ttagaaaaag
     9361 caactggtac tattaagcgt gaacttggca aaaaccttac tatgtataaa ataccagatt
     9421 tacaatttgt taaagatgaa tctattgaat acggaaataa gattgatgaa atgttgcgca
     9481 atctagataa aaaagattag aatcaagatt aagaataata aaaaataaac catgtcaaaa
     9541 tcatggttta ttttttatag ctttagagtt gagatagcta gtattaagga gttttattga
     9601 tgatatcaat catttgatac tgtgctgttt tagcttcttc aatagggtaa attggataaa
     9661 catgattcat tccctcttgt gcaatatagt taacggggtg attagctgct tggagttgtg
     9721 cagcataatc gcgaatatct gggaagaata tttctcgtgt tccagtaaat aacgtaatag
     9781 gagcgagttt agtagcaggg gcatttttag gactaacata tgtatggtta gtattattag
     9841 aaccatttgc ccaaatttca cctacacgtg cgagacccca agcagatagg ataggatcag
     9901 tatcttcgta tttgggaatt tctggatgtt tcattgtcac atctagccat ggagataaga
     9961 ggataatatt tttaggttga ggaatagcct cttgccctga ttggtgagac aatgcatgcg
    10021 ctaatcctag tgctaatcct ccgccagctg aatcacccat caaagtgaga ttagcgtttt
    10081 tttcatgaaa gtgtctataa aggttaacca atctagggat agcataatca taggtataac
    10141 gaggagtttt tggataaatt ggtaaaataa tttttgcatc aagactagtt gagagcgtct
    10201 taagcatgct gatatggtaa ggcgttggat tatttaagta agagccacca gctaaataga
    10261 aaatggtctt ttgattagct ttaccgttgt cattccaaga atagacttgc atatcaaaaa
    10321 gtctctcttg aacaagccct ccattaacag agtttgggtc taagtaataa cccttatcag
    10381 attgttcacg gctagcattt aagtactttt gaaatgagct ttcatcttga actttataat
    10441 aacttttatc gcgctttagc caaaagtatt gttcgataaa ccaacttttc catgaacgcc
    10501 cacttgcttt tgcatgtggt gtacaggcga tggataaaat ggtgataata ctaaatagta
    10561 gcaatagttt tttgaaggac ggtttcaagg tattctcctt aatataatgt tattttttgt
    10621 gtaatattat tataataaaa ccaacatctt tgtcaataga ataaagaaag atgagaaatg
    10681 tttttcggtt tacaaatgta gatttatttg ctaaaattgg tttacaaacg taaatcgaaa
    10741 gaggtgagaa aatgactatt tcaagcgcag aatgggaaat tatgagagtg gtttgggcac
    10801 agcagaatac aaccagtaat gagatactag cagttttact agagaagtat gattggactc
    10861 cttcaactgt caaaacctta ttaagaaggc tcttagataa aggctacgtg agcagagaaa
    10921 aaatgggaaa aggatttagc tattctcctt taattgatga agatttagct atgatgtcag
    10981 aggtagatag tgtatttcaa aaagtttgcc agaccaaaca tgttgctatt gttagacact
    11041 tattggaaag tataccaatg acagagaagg atcgattaaa tttgcaatca tcactagaag
    11101 ctaagaaagg caaaacactt gaacgtgtag cttgtaattg cattcctggt cagtgtcaat
    11161 gccattagaa aggagaaaat atggctaata gaaaagaaac atttttgatt gatgggatga
    11221 cttgtgcatc ttgtgcttta acgatagaaa aagctgtcaa caagttagac catgtggata
    11281 gtgcagtagt aaatttagca acagagaaaa tgacagttac gtttgatgat acaaccttat
    11341 cgccaaatgt gattgaagag tgtgtttctg agagtggata cgaagcatcg cttttcaaag
    11401 aggaaacttc taaaagccaa tctgaacggc accaattagc cattgaaaag atgtggcatc
    11461 gcttttggat gtcagcagtt gcaactatac ctcttctcta tatttccatg ggaccaatga
    11521 ttaacttatg gttaccatca tttttgatgc cagataaggg acctctaaac tatggtatga
    11581 tacaattatt gttaacacta ccggtgatgt attttgggcg tatcttttac caaaatggtt
    11641 ttaaagcact ttttaaaagg catcccaata tggattcatt agttgctata gccacaacag
    11701 cggctttcat ttatagctta tatggattgt acgaaatatt acagggtgac atacactacg
    11761 ctcaccagct ttatttcgaa tctgttgcag ttattttaac acttattact ctaggaaaat
    11821 attttgaaat attatccaaa gggcgtactt cggcctcgat tgaaaaatta ttaactttat
    11881 cagcaaaaga agcaagagtt atcaaagatg gtgaagacta tatggttcct ttggataaag
    11941 ttaaaatagg tgaaacaatt cttgtcaaac ctggagaaaa aattcctctt gatggccatg
    12001 ttgtagcagg agaatcaagt attgatgaat ccatgttaac aggtgaaagt attccagttg
    12061 aaaaaaaggt tggtagtaag gtttacggtg ccagcattaa tgggcaaggt agcttaacga
    12121 ttttcgttga aaaagaagct ggcggttccc ttctatcaca aattattaac ttagtagaag
    12181 ctgcacagac aagtaaggct ccaattgcta acttagcaga caaagtatca ggagtctttg
    12241 tcccttttgt tattgtcatt gcaattttat caggcttgag ttggtactta attctggggc
    12301 aaagttttgc cttctctctg aagattatga ttgcagtctt agtgattgct tgtccatgtg
    12361 ctttagggtt ggcaactcct acagctatta tggtggctag tggtaaagca gctgagaatg
    12421 gtattttatt taaaggtggt gaagtgttgg aaaaggctca ccacattgat acaatagttt
    12481 ttgataaaac aggaaccttg accaaaggga aaccagaagt agttgctatc aaaacatatg
    12541 ggggagataa agaggagttt ttaggtcagg tagcatcagt agaaaaactt tcaaatcatc
    12601 cacttagcca aacaattgtt aataaagcaa aagagaaaga acttccctta agggaagtca
    12661 tggcttttaa aaatattttg ggttacggtt tgagtgcaac tattaatggt aaaactatgc
    12721 ttgtaggtaa tgctaactta atgactaaga acgatgttaa tttagattta gcaaaggctg
    12781 atatagaaat agctcaagaa gaagcacaaa ctgtggttta cgtttccgaa aatggagtgt
    12841 tgagcggttt aataacttta acggaccagc taaagacaga tagccaagaa acagttaagc
    12901 agttacaaag attaggattt aatttagttt tacttactgg tgataataag gctagtgcag
    12961 atgccatagc acaaaaattg ggtatcacga cagttgtttc agaagtttta cctgatcaga
    13021 aagcaaacgt tattttagaa cttaaagaaa aaggcggaca gattgcgatg gttggtgatg
    13081 gcatcaatga tgctcctgcg ctagctagca gtgatgtagg tatctctatg agttctggaa
    13141 cagatattgc tattgaatca gcagatatcg tgttgatgaa acctgagtta acagatctct
    13201 taaaagcaat gactatcagt aaacaaacga ttcaaattat taaggaaaat ttattttggg
    13261 ctttctttta taacgtttta gcgattccag tggcaatggg tgttttacat ctttttggtg
    13321 gtcccttact caatcctatg ttggcaggct tagctatggc gtttagttca gtttcagtag
    13381 ttttaaacgc acttcgctta aaagtcttaa aataataaag ataataagac tgaattttta
    13441 tgaagggaga agaatatgaa acatacctat cgagttagtg gcatgaaatg tgatggttgt
    13501 gccaaaacag tttctgataa gttatcaagt gtgattggtg ttgacgaggt taatgttgat
    13561 ttgactaaaa atcaagttgt cgtaagtgga aaaacattca aatggctttt aaaacgttct
    13621 ttaaaagaca caaagtattc actagaagag gaaataaatt aataactaaa gaagtggtta
    13681 cagcttttaa tttcttaatt tgagcagttt ctcacttctt ttttattcta aaaatgataa
    13741 actagaatag aattagttgt gaggagtgca gatgtcaatt gatatttgga acattttaag
    13801 tttggttgga accgtagctt ttgcaagttc gggagctatt gtggcgattg aggaagaatt
    13861 tgatattcta gggcttttta tcttaggttt tgtaacggca tttggtggtg gagctattag
    13921 aaatgtacta attggcttgc ctattgaaac actttggagt caaggaattg ctttttatgc
    13981 agcagcagca gctattttat ttattatgat tttccctaat ttgttgagtg gtaagggacg
    14041 ggatgccgaa gttgtatcag atgctattgg gttagcagcg tttagtgttc aaggagcact
    14101 atatgcgact caatctcatc aacctcttag tgctgtcatt gtcgcagcag tactaactgg
    14161 tgcaggtggt ggtattgtca gagatgttct ggctggccga aaacctggag tactaagaag
    14221 tgaaatatat gcaggttggt caatattagt ggggatcatt ttatatttta aaattgctaa
    14281 aacaacaact gactattatt tgttagtgtt agttgtaact agcctaagga tgttgggtta
    14341 taagaaacaa tggcacttgc cagttgtcag accacgtaag aaatgataga ggagaaaaag
    14401 atgataaaat taattgctat tgatatggat ggaacgcttt taaatgatga aaagaaaatt
    14461 ccaaaggaaa atatccaagc tattaaagaa gcaactcaag caggtattaa aattgtactg
    14521 tgtactgggc gaccgatgtc aggtatactt ccttatttta atgaactagg tttaacaaaa
    14581 gaggagtata ttatcatgaa taatggttgt agcacttata gcacgaaaga ttggcaattg
    14641 attgattctg caactttaac acatgatgaa ctcatctttc ttgaggaggt tgttaaagaa
    14701 tttcccaacg tttgtttaac attaacagct gaaaatacat tttacgctgt tggagaggaa
    14761 gttcctgaaa ttgttgctta tgatgctgat ttagttttta caaaagcaaa gtcaacaagt
    14821 ttagatgctc ttcgcaatca agaagaaatt gtctttcaag caatgtacat gggattagat
    14881 gcagatgtaa ctgcttttca agaagctgtt gaagaagcct taatctcgaa atttagtgga
    14941 gttcgaagcc aagactatat ttatgagatt atgcctcaag gagtaacaaa agcaagaggc
    15001 ttaaaatcct tgatagcaaa attaggactg gatattaatc aagttatggc catcggtgat
    15061 gctcctaacg atattgaatt attagattta gttcccaata gcgttgctat ggggaatgca
    15121 tcagatgaga ttaaatctag atgtaaatat ataacggtgg ataataacaa agcaggggtt
    15181 gctaaagcta tttatgacta tgctttacgt taaaatagat atagaataat aaagggaact
    15241 ttaaacactg tttgtttagg gttcttttgt ttaataaact aacaaaattc agaagtttac
    15301 agatagctta tgctataata gacctatgac aaacaagaat aaattacttt taattgatgg
    15361 ctcatctgta gcctttcgtg ctttttttgc tctttataat caaattgacc gctttaaaaa
    15421 caattctgga cttcatacta atgccatata tggtttccat ttgatgttga atcatatttt
    15481 aggtcgtgtc cagcctagtc atattttagt ggcttttgat gccggaaaaa ctactttccg
    15541 tacggaaatg tatgcagatt ataaaggtgg gcgtgctaaa actccggatg aatttcgtga
    15601 acaatttcct tacattcgtc aacaattaga tgtcttaggc atcaaacatt atgaattgga
    15661 acattatgaa gccgatgata tcattggtac tcttgcaaaa caagccgaag ctagtaatga
    15721 acattttgat ataacggtgg tttctgggga taaagattta attcaattaa cagacactaa
    15781 cacggtagta gaaatctcta aaaagggagt tgctgagttt gaagaattca cgccagctta
    15841 ccttatggaa aaaatgggaa ttacgccgag tcaatttatt gatttaaaag ctttgatggg
    15901 agataaatca gataatattc ctggggttac taaaatcggt gaaaaaactg gcttgaagtt
    15961 gctatcagaa tatggttcct tagaagggat ctatgaaaat attgaggcta tgaaacagtc
    16021 taagatgaaa gaaaacctta tcaatgataa ggagcaagct ttcttatcaa agaccctagc
    16081 tacaatcaac atagcatcac ctataacaat cggcttagaa gatatccttt atagtggacc
    16141 gcaagatata aaagccttgt ctcaatttta tgatgagatg gattttaaac agtttaaagc
    16201 agctctgggt gaggagacaa gtcaggaaga ttttgaggtt gactttactg aagtggagca
    16261 attaaaaaca gaaatgttct ccgataatga tttttattac tttgagatgt tgggtgataa
    16321 ctaccatgta gaagatttaa ttgggatagc ttggggaaat agcgatacaa tctacgctac
    16381 tagtaatgta tcgttattgc aagaagcttt atttaaaaaa gctttatcaa aacctatcaa
    16441 aacttatgat tttaaaagaa gtaaggtcct tttaaatcgt tttaatattg atttaccaga
    16501 gccagccttt gatactagat tggccaagta ccttttgtct actactgagg ataatctagt
    16561 atcaacaatt gctaggttat atacaaatct gccactggat actgatgatg ctgtttatgg
    16621 gaaaggtgct aaacgcgcta taccagaaaa aacacgattt cttgagcatc tagctaaaaa
    16681 agtaaaagtt ttagtggatt ctgaggctaa tattatgcaa cagcttaaag ctaatgagca
    16741 agaagaattg ctgtttgaaa tggagcaacc tttagcgaat gttttagcta aaatggaaat
    16801 tcgaggtatt aaggttaaga aaaatacctt gaatgaaatg gccattgaaa atcaaaaagt
    16861 catcgaaaca ttaacacaag aaatctatga attggctggg caagagttta atattaattc
    16921 tccaaaacag ttaggaaagt tgttgtttga aacgttaggt cttcctgtag aaatgactaa
    16981 aaaaacaaaa acaggttatt caactgcagt agatgtatta gagcgcttag ctccgatttc
    17041 tcctcttgta actaaaattt tagagtaccg tcaaatcact aaattgcaat caacctatat
    17101 tattggttta caagattata ttttagaaga tggtaaaatt catacacgtt atgttcaaga
    17161 tttgactcag acaggtcgtc tttcaagttc tgacccaaac cttcaaaata taccagttag
    17221 actagagcaa ggacggctta tccgaaaagc ttttgttcca tcagaagaca atgctgtttt
    17281 attgagttca gattattctc aaatagaatt gcgcgtgtta gcacatattt caaaagacga
    17341 acatttgatc gctgccttta aagagggagc tgatattcat acttcaacag ctatgcgtgt
    17401 ttttggtatc gaaaaacctg agaatgtcac tcccaatgat cgtcgaaatg ctaaggctgt
    17461 taattttgga attgtttatg gtatttcaga ttttggtcta tcgcataatt taggaatacc
    17521 tcgtaaacta gcgaagcaat atattgacac ctattttgag cgttatcctg gaataaaaaa
    17581 ctatatggaa acagttgttc gtgaagcaaa agataagggt tacgttgaaa cacttttcca
    17641 tcgtcgtcgt tcattaccag atattaattc tcgtaatttt aacattcgcc aatttgcaga
    17701 gcgcacagca atcaattcac caatccaagg aagtgcagct gatattctta aaattgcaat
    17761 gataaattta gatagggtac ttgataaagg tggttataaa tcaaaaatgc tgttgcaagt
    17821 tcatgatgaa attgtacttg aagtccctaa cgaggagata ggagcaatca gggagttggt
    17881 tacaaaaaca atggagtcag caattagttt atcggtacct ttgattgcag atgagaacgc
    17941 aggtgagact tggtatgaag cgaagtagtg gacaacatac gtaaaggagt cattaatatg
    18001 gtttatcatt ttcaaaatcc aagtgatttt atgttaaaaa attatttaac aaaagcaaag
    18061 accatcgcag ttgtaggact gtcagaccgt caagaaactg cagcttatca agtatcaaaa
    18121 ataatgcaag aagcaggtta tcaaattatt ccagttaatc ctaaaaatgc aggacagaaa
    18181 atacttgggc aaatgacata tgcctcactt aaggatgtca ctgaacatat tgatattgtt
    18241 aatatttttc gacgtagcga gtatttacct gatattgcaa gagaattttt agaagtagac
    18301 gctgatattt tctgggctca gcttggactt gaaagtcaag aagcagagac aattctaaag
    18361 caagcaggac acaaacagat tgtcatgaat aaatgtctga aagttgaatg ccaaaaatta
    18421 cactcagaaa ctaactaatt accgtgttta ataggcaaat aatataaatt atgctataat
    18481 aaccatgcta aattaaagtt cgtgctggaa aggagtccta tggacagtca ttcacatggt
    18541 catagacctc tcgatgctta tgaaaacgtc ctagagcatc tccgtgaaaa acgtattcga
    18601 attactgaga ctcgaaaagc tattatttcc tatatggtta atagtcgtga gcatccgagt
    18661 gcagaaaaga tttacaatga tttattacca gaatatccta acatgagctt ggcaacagtt
    18721 tacaataacc ttaaggtcct tgtggatgag gggtttgtga cagagctaaa actttgcaat
    18781 tatagtacaa cttattatga ttttatgggg catcagcacc taaacattgc ctgtgaagat
    18841 tgtgggaaaa tcgttgattt tgtcgatgtt gacttattgg atatttctag agaagcacac
    18901 caacaaacag gttttgaagt gactagagtt cagttagtag cttatggtat ctgtccggag
    18961 tgtcagagga agcgtaagga taaatataaa aaagattgaa acgtaattgt tcaatctttt
    19021 tttgtttata gactaagaga aaaagtttaa gttttactaa gtaataatta agaaataatt
    19081 tataattgct taaaaaatga tttatttttt gttggtatac tctcattaac aaaaagaaag
    19141 aggaatccct tatgaaaata tcacaataca ataaatggtc aattcgccga ttgaaagttg
    19201 gagctgcatc tgtaatgatt gctagtggaa gcattgttgc tttagggcaa tctcatattg
    19261 tttcagcaga tgagatgtcc caacctaaaa caaccattac agctccaaca gctaacactt
    19321 ctacaaatgt agaaagtagt actgataagg cattatcaaa agttactacg atggagacaa
    19381 gttcagaaat gcctaaaatg caaaatatgg caaaagttga aaaaacgagt gacaaaccta
    19441 tgatggtggc tacctctgtt agaaaaatga tggcaacgcc gacacctgta gcgatgacca
    19501 aaacgactag tgtagatgag gtaaagaaat caactgatac tgcttttaaa cagacagtag
    19561 acgttccggc tcattatgtt aatgcagcaa aagggaatgg cccatttcta gcaggtgtaa
    19621 atcaaacgat tccatatgaa gcgtttggtg gagatggcat gttaacgcgt ttaatcctta
    19681 aatcttctga aggagcaaaa tggtcagata atggtgttga taaaaatagt ccattattac
    19741 cattgaaagg cttaactaaa ggtaaatatt tctatcaagt ttctttaaat ggtaatacga
    19801 caggtaaaga gggacaagct ctcttagatc aaattaaagc taatgataaa catagctacc
    19861 aagccacaat tagagtttat ggtgctaaag atggtaaagt tgatttaaaa aacatgatta
    19921 gccaaaaaat ggtaaccatt aacattcctc acattacaac tgatatggaa gtaaaaaatt
    19981 ctctaaagat ggcctttaaa gaaaaagtag acgtcccagc aaaatatgtt agtgctgcaa
    20041 aagctaaagg tccattttta gcaggtgtta acgaaacgat tccatatgaa gcgtttggtg
    20101 gggatggcat gttaacgcgt ttaatcctta aagcttcaga gggagcaaaa tggtcagaca
    20161 atggtgttga taaaaatagt ccattattac cgttaaaaga tttaactaag ggtaaatact
    20221 tctaccaagt ctctcttaat ggcaatacag ccggtaaaaa aggccaagct ctcttagatc
    20281 aaattaaggc aaatggaagc catacttatc aagcaactat cactatttat ggtacgaaag
    20341 atggcaaagt tgatatgaat actattttag gacaaaagac agtgatgatt catattaatg
    20401 tcgctaaaaa agatatgaat agcacatcaa tgatgatgaa aaaagataag atgacaatgc
    20461 ctatgaagaa agaaatgact tcatctaaaa taaataccgg tatgatgatg tctaacaaca
    20521 agatgtcagc taatatgcaa atgtcatcac aagccaaatc aaatgataaa gctggtaaaa
    20581 aaatgtctat gatgtctaaa aatttaccta atacaggtga aacaaagcaa caaaatgtag
    20641 gtgtgctagg tatgcttagt ctagcctttg cgacaggtct aactgcgcta ggtcttaaaa
    20701 aatctaaaca aagataggga tagtatttaa ttcagaacac caaatttgtt taaaattatt
    20761 gagggttatc gtggtaaaat agtgttatac tagaggtata gcactatttt gactcattag
    20821 gagagtagaa tgacacaaaa attattatta gttgacgatg aatttgaaat tattgatatt
    20881 aatagacgtt atctagagca agcaggatat gaagtgtcag tagctgcgga tggcatagaa
    20941 gcactcaaag aggtagatga gaatcgtttt gacctaatta ttagtgatat tatgatgcct
    21001 aaaatggatg gctatgattt tattagtgaa gttctagttc gagaacccaa ccaacctttt
    21061 ctctttataa ccgctaaagt atctgaacca gataaaattt actctttgag tatgggtgca
    21121 gatgacttta tttccaagcc tttcagtcct agggaattag ttttacgtgt taaaaatatt
    21181 ctaagacgta tctatgggaa tcatcaacaa tctgaagtgt tgacgattgg tgatttggtt
    21241 atcgaccaaa aacaacgttt ggtaatggtg gactgcaata caatttcctt aaccaataaa
    21301 tcatttgact tgctttggat attagcaaat catttgaatc gtgttttttc aaaaacagag
    21361 ctttatgaac gtgtctgggg cgaagaattt ttagatgaca ctaatacttt gaatgttcat
    21421 atccatgcct tacgcaatga ccttgctaaa tttagtacag acaatacccc aactatcaaa
    21481 acagtttggg gattagggta taaattagag gaataaaatg aaattaaaat attatattgt
    21541 tattggttac cttatttcta tgttaataac tgtagcaggt gttttctttg ggcttaatca
    21601 tatgctgatt gagactcgag gtgtttacta catcctttca gttacaatta ttgcctgtat
    21661 tgtaggtggt atagttaatt tgttcttatt atcaagtgta tttacatctt tgaaaaagtt
    21721 aaaacaaaaa atgaaggata tttctcaaag atgttttgat acaaaagcac aaatttgttc
    21781 accacaagaa ttcaaggatt tagagacagc ctttaaccaa atgtcttcgg agttagagtc
    21841 aacgtttaaa tctctaaacg agagtgaacg cgaaaaaaca atgatgattg cacaactttc
    21901 acatgatatc aagactccaa ttacttctat acaatcaaca gtagaaggta ttttagatgg
    21961 tattatatct gaagaagaag ttaactatta tctcaatacc atttctcgtc aaacaaaccg
    22021 tcttaaccat ctggttgaag aattaagttt tattacatta gagactatgt cagacacggc
    22081 tgaacctcat aaagaagaaa caatttatct ggataaacta ctgattgata ttttatctga
    22141 atttcaattg gtgtttgaaa aagaaaatcg ccaagtaatg attgatgttg cgccagatgt
    22201 ttcgaaatta tctagtcaat atgataaatt atcacgaatt ttattgaatt tgattagcaa
    22261 tgcagtcaaa tattctgatc ctggaagtcc cttgactatc aaggcctatt caaatcgtca
    22321 agatatcgtt atcgatatta ttgaccaagg ctatgggata aaagatgaag atcttgcttc
    22381 tatttttaat cgtctttatc gtgtagaatc ttcacgaaat atgaagacag gaggacatgg
    22441 tctaggtctt tatattgcgc gacaacttgc acatcaactg aatggggata ttttagtcga
    22501 aagtcagtat caaaaaggca gtaagttctc tttagtactt aaattacaaa aatagctagg
    22561 aattattcct agctattttt tgtaatgttt ctataaaaga ttaagcgcgc agtagtttgg
    22621 taaggaagga gatagatata gactaatccg aaactgaaga ttgtaaggaa ctgccaacca
    22681 ataaaactta agtctaaaag gaagagtcta agtttattac cattcattag ttctcgacta
    22741 gcaaccagta ctttagacgg tccctcatag tttccacttt ctaattgagt gtaaaatact
    22801 aaagttgaga tactatagga gtaatattgt tgaacgagtt ttataactgc cttagtagta
    22861 attaggctac taacaactat taaaacaggg gcaaggataa ggttaccact caaaagtagc
    22921 ctaaaacttg aaatgatgat tagtagacca aaagtactta gtagataaaa gaaagataaa
    22981 attagtcctt ttagaacaag tactgagagt aatttccaga aaatatggct tgaaaataca
    23041 taagtattgt ccgagaatct aacgtttagt ttctgatttt tgacgacatc taacattaca
    23101 aaactgactg aaataataaa aagtgtgtaa aagaaataaa ctggaagagg tacaaaaaaa
    23161 tcaagtagtg tcatattatt atattgataa ta
//
LOCUS       DATRMA010000027        22282 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.027, whole genome shotgun sequence.
ACCESSION   DATRMA010000027 DATRMA010000000
VERSION     DATRMA010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22282
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.027"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            127..627
                     /locus_tag="VBC28_000994"
     CDS             127..627
                     /locus_tag="VBC28_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000752178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO6814993.1"
                     /translation="MACIIRRARLGDEVNLAYIQTESWKAAFGKILPEDIIQKTTEIE
                     PAITMYQQLLHKEVGKGYILEVDSNPHCMAWWDKSREDGMLDYAELICIHSLKEGWGK
                     GYGSQMMNHVLSEIQQAGYNKVILWVFTENTRARKFYDRFGFSFKGKSKTYFEKEEMC
                     YEKILH"
     gene            825..1073
                     /locus_tag="VBC28_000995"
     CDS             825..1073
                     /locus_tag="VBC28_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6814994.1"
                     /translation="MEKITNDFEKVIAVLNQLSISYELVENGPVLLTEQADNFIVGIE
                     GVRTKIMFLTNKKKTKYYLLIMDDQKLFYPMKTIVAFR"
     gene            1163..1399
                     /locus_tag="VBC28_000996"
     CDS             1163..1399
                     /locus_tag="VBC28_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018364244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HEO6814995.1"
                     /translation="MTFEEILPGLKAKKKYVRTGWGGAENYVQLFDTLEVNGKVLQAT
                     PYFLINVTGEGEGFSMWAPTPCDVLAEDWIEVND"
     gene            1392..2090
                     /locus_tag="VBC28_000997"
     CDS             1392..2090
                     /locus_tag="VBC28_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HEO6814996.1"
                     /translation="MTKVVLVTGCASGIGYAQAQYFLKQGYQVYGVDKSDKPNLNGNF
                     NFIKLDLSSDLSPLFTMVPTVDILCNTAGILDAYKPLLEVSDEELEHLFDINFFVTVR
                     LTRHYLRRMVEKKSGIIINMCSIASFIAGGGGAAYTSSKHALAGFTRQLALDYAKDCI
                     QIFGIAPGAVQTAMTASDFEPGGLAEWVASETPIGRWTKPSEVAELTGFLASGKARSM
                     QGEIVKIDGGWSLK"
     gene            2087..2332
                     /locus_tag="VBC28_000998"
                     /pseudo
     CDS             2087..2332
                     /locus_tag="VBC28_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233822.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3977 family protein"
     gene            2329..2780
                     /locus_tag="VBC28_000999"
                     /pseudo
     CDS             2329..2780
                     /locus_tag="VBC28_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647214.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
     gene            2777..3733
                     /locus_tag="VBC28_001000"
     CDS             2777..3733
                     /locus_tag="VBC28_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEO6814997.1"
                     /translation="MKLAVLGTGMIVKEVLPVLQKIEGIDLVAILSTVRSLETAKDLA
                     KEYNMSLATSEYKAVLDNEEIDTVYIGLPNHLHFDYAKEALLAGKHVICEKPFTLEAS
                     QLEELVSIANTRQLILLEAITNQYLPNFDLVKEHLSNLGDIKIVECNYSQYSSRYDAF
                     KRGEIAPAFNPEMGGGALRDLNIYNLHLVIGLFGEPITAQYLPNIERGIDTSGVLVLD
                     YGHFKTVCIGAKDCSAEVKSTIQGDKGSIAILGPTNTMPKISLTMNGQESHVYQLNGD
                     RHRMHDEFVIFEGIISNLDFKRAAQALEHSRTVMKVLDMVTK"
     gene            3822..4198
                     /locus_tag="VBC28_001001"
                     /pseudo
     CDS             3822..4198
                     /locus_tag="VBC28_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625774.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            4211..4624
                     /locus_tag="VBC28_001002"
     CDS             4211..4624
                     /locus_tag="VBC28_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEO6814998.1"
                     /translation="MVKALETYIVTNGNGRQAVDFYKDVFQADLVNMMTWEEMDPNCL
                     EDRKDLIINAQLIFDGIRLQISDENPDFVYQAGKNVTAAIIVGSVEEAREIYEKLKKS
                     AQEVQLELQETFWSPAYANLVDQFGVMWQISTELN"
     gene            4868..6247
                     /gene="glmU"
                     /locus_tag="VBC28_001003"
     CDS             4868..6247
                     /gene="glmU"
                     /locus_tag="VBC28_001003"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEO6814999.1"
                     /translation="MSNYAIILAAGKGTRMKSDLPKVMHKVSGITMLEHVFRSVQAIE
                     PSKIVTVIGHKAELVRDVLGDKSEFVMQTEQLGTGHAVMMAEEELATSKGHTLVIAGD
                     TPLITGESLKNLIDFHVNHKNVATILTADAANPFGYGRIIRNSDDEVTKIVEQKDAND
                     FEQQVKEINTGTYVFDNQSLFEALKDINTNNAQGEYYLTDVIGIFKEAGKKVGAYKLR
                     DFDESLGVNDRVALATAEKVMRHRIARQHMVNGVTVVNPDSAYIDIDVEIGEESVIEP
                     NVTLKGQTKIGKGTLLTNGSYLVDAQVGNDVTITNSMVEESIISDGVTVGPYAHIRPG
                     TSLAKGVHIGNFVEVKGSQIGENTKAGHLTYIGNAEVGCDVNFGAGTITVNYDGQNKF
                     KTEIGSNVFIGSNSTLIAPLEIGDNALTAAGSTITDNVPIDSIAIGRGRQVNKEGYAN
                     KKPHHPSQK"
     gene            6268..6822
                     /locus_tag="VBC28_001004"
     CDS             6268..6822
                     /locus_tag="VBC28_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEO6815000.1"
                     /translation="MDFEEKTINRQTVFDGQIIKVAVDDVELPNGLGQSKRELVFHGG
                     AVATLAVTPEHKIVLVKQYRKAIEGISYEIPAGKLETGESGSKEEAALRELEEETGYT
                     GNLEILYSFYTAIGFCNEKIVLYLATDLQKVENPRPQDDDEVLELLELSYEDCMQMVE
                     KGMIQDAKTIIALQYYGLKMGGHS"
     gene            6822..7091
                     /locus_tag="VBC28_001005"
     CDS             6822..7091
                     /locus_tag="VBC28_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6815001.1"
                     /translation="MGKPLLTDDMIERSNRGEKVSGQTILDQETKIISTEDGMEQLTD
                     ENGKHIYKSRRIENAKRNEFQRKLNLVLFILLILLALLFYAIFKL"
     gene            7101..7790
                     /locus_tag="VBC28_001006"
     CDS             7101..7790
                     /locus_tag="VBC28_001006"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEO6815002.1"
                     /translation="MKIGIIAAMEEELKLLVENLEDKSQETVLSNVYYSGRYGEHELV
                     LVQSGVGKVMSAMSVAILVESFKVDAIINTGSAGAVATGLNVGDVVVADTLVYHDVDL
                     TAFGYDYGQMSMQPLYFHSDKTFVSTFEAVLSKEEMISKVGLIATGDSFIAGQEKIDV
                     IKGHFPQVLAVEMEGAAIAQAAQATGKPFVVVRAMSDTAAHDANITFDEFIIEAGKRS
                     AQVLMAFLKAL"
     gene            complement(7830..8477)
                     /locus_tag="VBC28_001007"
     CDS             complement(7830..8477)
                     /locus_tag="VBC28_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000188484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEO6815003.1"
                     /translation="MTPNKADYLKCIHELGEKNNKISNKKIAEMMQVSAPAVSEMIKK
                     MISEQLIVKDKDLGYYLTKQGLLVVSDLYRKHRLVEVFLVNHLHYTADDIHEEAEVLE
                     HTVSTTFVDQLEKLLDFPQFCPHGGTIPKKGEFLVEINQMTLDQISQLGTYVISRVHD
                     DFQLLKYLEQHRLHINDTIELTQIDPYAKTYHITYNDENLTIPERIASQIYVSAT"
     gene            8644..9570
                     /locus_tag="VBC28_001008"
     CDS             8644..9570
                     /locus_tag="VBC28_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HEO6815004.1"
                     /translation="MKKWLVIVSCFVAFLGLGACANKQDQAKNEKLKVVVTNSILADI
                     TKNIAGNKIDLHSIVPVGKDPHEYEPLPADVEKTTQADLVFYNGINLETGGNAWFTKL
                     IKNAKKKENKDYFAVSDGVDVIYLNGQSGKGKEDPHAWLNLENGIIYSKNIAKQLIAK
                     DPKNKATYEKNRDAYVAKLEKLDKEAKSKFNAIPANKKLIVTSEGCFKYFSKAYGVPS
                     AYIWEINTEEEGTPDQITSLVKKLKQVRPSALFVESSVDKRPMKSVSRESGIPIYAEI
                     FTDSIAKKGQKGDSYYAMMKWNLDKIAEGLAK"
     gene            9741..10457
                     /locus_tag="VBC28_001009"
     CDS             9741..10457
                     /locus_tag="VBC28_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEO6815005.1"
                     /translation="MIISKHLSVSYDNNLVLEDINLRLEGSGIIGILGPNGAGKSTLM
                     KALLGLVDSTGESGIGGDLLPLMGRVAYVEQKTNIDYQFPITVGECVSLGLYKERGLF
                     KRLSKTDWEKVSRVIDQVGLRGFENRPINALSGGQFQRMLMARCLVQEADYIFLDEPF
                     VGIDSISEQIIVNLLKKLSKAGKLILVVHHDLSKVDHYFDQVIILNRHLIACGPIDQA
                     FTRENLSAAYGDAILLGQGD"
     gene            10459..11292
                     /locus_tag="VBC28_001010"
     CDS             10459..11292
                     /locus_tag="VBC28_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEO6815006.1"
                     /translation="MFTKFFEGLLTYHFLQNAFITAIVIGIVAGAVGCFIILRSMSLM
                     GDAISHAVLPGVAISFILGINFFIGAIVFGLLSSIIITYIKENSVIKGDTAIGITFSS
                     FLALGIILIGLANSTTDLFHILFGNILAVQDSDKYMTIIVGLIVLTLITIFFKELLLT
                     SFDPVLAKSMGMRVSFYHYLLMILLTLVAVTAMQSVGTILIVALLITPAATAYLYVKS
                     LRTMLFLSSALGAVASVLGLYIGYTFNIAAGSSIVLTSTFMFLLAFLFSPKQSLFKKK
                     Q"
     gene            complement(11344..12147)
                     /locus_tag="VBC28_001011"
     CDS             complement(11344..12147)
                     /locus_tag="VBC28_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEO6815007.1"
                     /translation="MKKIIYLGLACVSILTLSGCESIERSLKGDRYVDQKLAENSSKE
                     ATEQLNKKTKQALKADKKAFPQLDKAVAKNEAQVLIKTSKGDINIKLFPKYAPLAVEN
                     FLTHAKEGYYNGLSFHRVIKDFMIQSGDPNGDGTGGKSIWNSKDKKKDSGNGFVNEIS
                     PYLYNIRGSLAMANAGADTNGSQFFINQSQQDHSKQLSDKKVPKVIIKAYSEGGNPSL
                     DGGYTVFGQVISGMETVDKIASVEVTKSDQPKEKITITSIKVIKDYKFK"
     gene            12313..14763
                     /locus_tag="VBC28_001012"
     CDS             12313..14763
                     /locus_tag="VBC28_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000231573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEO6815008.1"
                     /translation="MVFMANKKKTKGKKTRRPTKAEIERQRAIQRMITALVLTIILFF
                     GIIRLGIFGITVYNVIRFMVGSLAYLFIAATLIYLYFFKWLRKKDSLVAGFLIASLGL
                     LIEWHAYLFSMPILKDKEILRSTARLIVSDLMQFKITVFAGGGMLGALIYKPIAFLFS
                     NIGAYMIGVLFIILGLFLMSSLEVYDIVEFIRAFKNKVAEKHEQNKKERFAKREMKKA
                     IAEQERIERQKAEEEAYLASVNVDPETGEILEDQAEDNLDDALPPEVSETSTPVFEPE
                     ILAYETSPQNDPLPVEPTIYLEDYDSPIPNMRENDEEMVYDLDDDVDDSDIENVDFTP
                     KTTLVYKLPTIDLFAPDKPKNQSKEKDLVRKNIRVLEETFRSFGIDVKVERAEIGPSV
                     TKYEIKPAVGVRVNRISNLSDDLALALAAKDVRIEAPIPGKSLIGIEVPNSEIATVSF
                     RELWEQSDANPENLLEVPLGKAVNGNARSFNLARMPHLLVAGSTGSGKSVAVNGIISS
                     ILMKARPDQVKFMMIDPKMVELSVYNDIPHLLIPVVTNPRKASKALQKVVDEMENRYE
                     LFSKIGVRNIAGYNTKVEEFNASSEQKQIPLPLIVVIVDELADLMMVASKEVEDAIIR
                     LGQKARAAGIHMILATQRPSVDVISGLIKANVPSRIAFAVSSGTDSRTILDENGAEKL
                     LGRGDMLFKPIDENHPVRLQGSFISDDDVERIVGFIKDQAEADYDDAFDPGEVSETDN
                     GSGGGGGVPESDPLFEEAKGLVLETQKASASMIQRRLSVGFNRATRLMEELEAAGVIG
                     PAEGTKPRKVLMTPTPSE"
     gene            14845..16560
                     /gene="pabB"
                     /locus_tag="VBC28_001013"
     CDS             14845..16560
                     /gene="pabB"
                     /locus_tag="VBC28_001013"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HEO6815009.1"
                     /translation="MHIETVIDFKELGKRYRFKNPTKELIADTLEQVLEVIKEVDYYQ
                     SQNYYVVGYLSYEASAAFDSHFKVSQQKLAGEHLAYFTVHKDCENEAFPLSYENVRLA
                     DNWTANVSEQEYQEAIANIKGQIRQGNTYQVNYTLELSQQLCSDPFSVYERLMVEQGA
                     GYNAYIAYDDKRILSVSPELFFKKKDEVLTTRPMKGTSARKPTYQEDVAERDWLANDP
                     KNRSENMMIVDLLRNDMGRICDVGTVKVKKLCQVEQYATVWQMTSTIEGVLSPEVTLM
                     SIFQALYPCGSITGAPKISTMAIINELEKRPRGIYCGTIGLCMPDGQAIFNVPIRTVQ
                     MKGQQAYYGVGGGITWESQTDSEYEETRQKSAVLTRVNPKFQLITTGRVTENKLLFSQ
                     QHVERLVESASYFAYSFDKSKFERELKKYLHQLDEKDYRLKIMLDKTGKVTFEVKQLV
                     NLSKKFLTAEVVVQDYPIKLSPFTYFKTSYRPHIIEGQNEKIFVSPEGLLLETSIGNI
                     VLEKNGRFLTPDLSEGGLNGIYRRHLLKNQKVIEAPLTLKDLESADAIYACNAVRGLY
                     PLNLK"
     gene            complement(16609..17954)
                     /locus_tag="VBC28_001014"
     CDS             complement(join(16609..17513,17513..17954))
                     /locus_tag="VBC28_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611514.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS861 family transposase"
                     /protein_id="HEO6815010.1"
                     /translation="MKLSYEDKLEIYELRKIGMSWSQISQRYDVRISNLKYMIKLMDR
                     YGVEIVEKGRNEYYPPELKQEMIDKVLIHGCSQLSVSLDYALSNCSILTNWLSQFKKN
                     GYTIVEKTRGRPSKMGRKRKKTWEEMTELERLQEENERLRTENAFPKKVEGFALEGRS
                     LTERTAETIREMVTGGFRLDLLLEITKIARATYYYQLKKLNKPNKDKAIKSDIQSIYD
                     EHRGNYGYRRIYLELRNRGFVINHKRVQGLMKSMGLTARIRRKRKYASYKGEVGKKAD
                     NLIQRQFEGSKPYEKCYTDVTEFALPEGKLYLSPVLDGYNSEIIDFTLSRSPDLKQVQ
                     TMLERAFPAASYSETILHSDQGWQYQHKSYHQFLEDKGIRPSMSRKGNSPDNGMMESF
                     FGILKSEMFYGLEKSYKSLDDLEQAITDYIFYYNNKRIKAKLKGLSPVQYRTKSFT"
     gene            complement(18025..18378)
                     /locus_tag="VBC28_001015"
     CDS             complement(18025..18378)
                     /locus_tag="VBC28_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HEO6815011.1"
                     /translation="MIYKIIASLFLVLIPIFSQVLVKIFKLKKFNIMFPDVAFPIFVF
                     LIPLISSSLLKQNLLPYYLILISLLAIGITIKLLRTKTLFSYKRFLKLFWRSGFILTF
                     LFYLGLLVIIFIKVQ"
     gene            complement(18439..19344)
                     /locus_tag="VBC28_001016"
     CDS             complement(18439..19344)
                     /locus_tag="VBC28_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEO6815012.1"
                     /translation="MKNQQKLMYLESIELYSNITKAAAHLFISQPYLSKVIKQLENEL
                     EIKLIQSQGHQTFLTYAGQRYLFYLKEIDMIERQMAKELYLIRSDKKGEITLGINSGL
                     ASSILANVLPKFNLEHPEISVKLLENNQNISEQLVASGDIDLAVGMAPILYKDGIAST
                     TIYRDELFLMIPTTSQLYNAEKRGQIIPFEYPISVLDNEPLILTPLEYGIGKTIAQFY
                     ELHHMSLNQMITTSTVPTAASLSLSGMGATFVPQTLIHRYLDKECNVYHFHKNKLFSE
                     YIMIYKKDVELSGIALLLYKAFLTK"
     gene            19602..20816
                     /locus_tag="VBC28_001017"
     CDS             19602..20816
                     /locus_tag="VBC28_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEO6815013.1"
                     /translation="MANASLRHQLFEKLDQKCDQMVAIRRYLHENPELSFKETKTAAY
                     ISDFYKGKDCHVQTQFGGMNGVVVDIYGDKATDKPIKHIALRADFDALPIQEETGLSF
                     ASKTAGVMHACGHDAHTAYLLILAESLIELKSEFSGHIRILHQPAEEVPPGGAKAMIE
                     AGCLDGIDAVLGIHVMSTMEEGTVQYHAGPIQTGRATFKVILQGKGGHGSMPHRANDT
                     IVAASSFVMAAQTIVSRRVNPFDTAVVTIGSFDGKGSANVIKDSVTLEGDVRVMSEET
                     RGVVEEEFKRILDGIAQTYGVSYQLDYQNDYPVLVNNSEVTQKVANSLKSVAIKEILD
                     VIDCDPQTPSEDFAYYAQTIPACFFYVGAHEEGQPYYPHHHPKFQIAESSLMVSAKSM
                     ATAALAMLVEGE"
     gene            20824..22206
                     /locus_tag="VBC28_001018"
     CDS             20824..22206
                     /locus_tag="VBC28_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000958256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO6815014.1"
                     /translation="MLTTKENFKGNNKLLVGIVLAVLSFWLFAQSILNMGPDVQSSLG
                     ISSGAMDIGVSSTALFSGLFIVVTGGLADKLGRVKFTFIGLCLNIIGSLLIVLANGAV
                     LFIMGRIFQGLAAAFIMPSTMALVKTYYDGKDRQRAVSFWSIGSWGGSGLCSYFGGAV
                     ASTLGWRYVFIFSIIASVVSFLLILGTPESKNVGQKTHFDYLGLIIFIISMLSLNIGI
                     SMAQEHGLMNVIPLSLFTVMLIGFVLFYYVETRKSNSFIDFHLFENRFYLGATISNFL
                     LNAVAGTLIVINTYMQQGRQLTPKVAGEMSLGYLVCVLIAIRVGEKILQRFGARKPML
                     LGAMSTFVGIFLMTLVNIQGPLYLVLVFVGYALFGTGLGIYATPSTDTAISSIPNEKV
                     GSASGIYKMASSLGGAIGVATSIAIYHAFSGNADFHKAALCGLILNLVFCSLSILSIL
                     FVIPKKIEDK"
BASE COUNT         7235 a         3304 c         4516 g         7227 t
ORIGIN      
        1 aaaattttag aaatataatg ataaatgaga accagaactg ttcaaaataa aaattaatat
       61 cacgaaaatg gtataattgg agtagttatc aggcagttaa taataatttt tgaaaggatt
      121 ttagttatgg cttgcataat caggagagca agattaggag atgaagtcaa tttagcttat
      181 attcaaacgg agagctggaa agctgctttt ggtaagatac tacctgagga tatcattcaa
      241 aaaactacgg aaatagaacc tgccataaca atgtatcaac agctactaca caaggaggtt
      301 ggtaaggggt atatattgga agtagatagc aatcctcatt gtatggcttg gtgggataaa
      361 agtagagaag atggtatgct tgattatgca gaactcattt gtatacatag tcttaaagaa
      421 ggttggggaa aaggttatgg gtctcaaatg atgaatcatg tactatcaga gatacaacaa
      481 gcaggttata ataaagtgat actttgggta tttacagaaa atacacgcgc aaggaaattt
      541 tacgataggt ttggtttttc ttttaagggt aaatcaaaaa cttattttga aaaggaagaa
      601 atgtgttatg agaagatttt gcactagcga gataaagcat ccgataatac aatagtataa
      661 acatcctatt ttgaaaaaca atttccttta aggggaacaa atttgtaaaa gaaaattgtt
      721 ataggtataa tgaagattat gaaggtaaaa ttttttgagt gaaaaacagt gtatcaattt
      781 taggttctct agtctacaaa tagttatgct aactctatgg aagaatggag aaaattacga
      841 atgattttga aaaagttata gctgtgctaa atcaactaag tatttcttat gaattagtgg
      901 aaaatggacc tgttttgttg acggagcaag cagataactt tattgtggga attgaaggag
      961 taagaacaaa aataatgttc ttgaccaaca agaaaaagac gaaatattat ttactaataa
     1021 tggatgacca gaaactattt tatcctatga aaacaatagt agcctttaga tgaactaaaa
     1081 ggcgaagaaa ctttaaatta tgattttaaa taagcaataa accttgatat atcaatggtt
     1141 aaagacaata aaggagaaca atatgacatt tgaagaaatt ttaccaggtt taaaagcaaa
     1201 gaaaaaatat gtacgtacag gttggggggg cgctgaaaat tatgttcagt tatttgatac
     1261 ccttgaagtt aatgggaaag tactacaggc tacaccttat tttcttatta atgttactgg
     1321 tgaaggtgaa ggtttttcca tgtgggcacc aacaccatgt gatgttttag cagaggattg
     1381 gatcgaggtc aatgactaaa gttgttttag tgacgggctg tgctagtgga attggttatg
     1441 cacaggctca atattttttg aaacaagggt atcaagtata tggtgtggat aagtcggata
     1501 aaccgaactt aaatggtaat tttaacttta taaagttgga tttatcctct gatttatccc
     1561 cactattcac aatggtaccg actgttgata ttttgtgtaa tactgcaggt attctagatg
     1621 cctacaagcc acttctagaa gtaagtgatg aagaactgga acacctattt gatattaatt
     1681 ttttcgtgac tgttaggcta acacgtcatt atcttagacg tatggtggaa aagaagtcag
     1741 gtattattat caatatgtgc tcaatagcaa gttttatagc gggtggcggt ggagctgcat
     1801 atactagttc taaacatgcc cttgcgggat tcacacgtca attggctttg gattacgcaa
     1861 aagattgtat tcaaattttt ggtatagcgc caggtgctgt acaaactgct atgactgcta
     1921 gtgattttga acctggtgga ttagcagaat gggtagctag tgagacgcca attggtcgtt
     1981 ggactaaacc aagtgaagtt gctgaattaa ctggatttct tgcttcagga aaagcgagat
     2041 caatgcaagg tgaaattgtc aaaattgatg gtggctggag tttgaaatga gaaaatagat
     2101 tgaatttggt tgtggtaata cttggttatt acgtacggaa tttgaagcat ctgatggtac
     2161 tgagcgggaa gttaaaggaa tatcaggtga tattttaata aaatctgttt atttacgtgt
     2221 ttggattggt caaactgttt atattttaga tgttaaagaa ggatttaaaa gacaaagaaa
     2281 aatgagtaac gcagttaagt ttgttgtggg aattgcttcg gaggtaatat gaaggcacaa
     2341 gaattatgga ataagtttaa atctttgaat ccttcaatcg gtgatgagat agacgcatgg
     2401 caatttggag cagaaccaga tcttctggca aagttagttg tagatgggac aaaaactgca
     2461 acggcctctg cctatgattt atatgaatta gatgaggaag ggttacacaa gttggttcat
     2521 atgatattat tttggatagc aatgatgaag ctatctgtat tattgaaata actaaagttg
     2581 atattgtccc ttttaaagat gtctctgcag accatgcttt taaagaagga gaaggagata
     2641 aaactttaga atggtggaga aaagctcaca ttgatttctt taaaccttat tttgaggagt
     2701 ttggtttaat gtttagtgag gactctagga tagttttaga ggaattccaa gtggtttacc
     2761 caaaggagat taacgaatga aattagcggt tttggggaca ggtatgattg ttaaggaagt
     2821 tttgcctgtc ttgcagaaaa ttgaaggtat tgatctagtt gctatcttat caacagttag
     2881 aagtttggag acagctaaag atctagctaa ggaatataac atgtctctag ctacgagtga
     2941 atataaggct gtcttggaca atgaggagat tgacacagtc tatattggac tgccgaatca
     3001 cttgcatttt gactatgcta aggaggcact cttggcagga aaacatgtca tttgtgagaa
     3061 gccatttaca ttagaagcaa gtcaattaga agaacttgta tctattgcca atactaggca
     3121 actaatatta ctcgaagcaa ttacaaatca atatttacct aattttgacc ttgtaaaaga
     3181 acatttaagc aaccttggag atattaaaat tgtagaatgt aattattccc aatattcatc
     3241 gcgttatgat gcctttaaac gtggggaaat agcaccagct ttcaatcctg aaatgggtgg
     3301 aggtgcgttg agagacctta atatttataa tttacattta gtaattggac tttttggaga
     3361 acctataaca gcacagtatt taccaaatat agagcgtggt attgatactt caggagttct
     3421 agtcctagac tatgggcact ttaaaacagt ctgtattgga gcaaaagatt gtagtgcaga
     3481 agtaaaatct actattcaag gtgataaggg gtctatagcg attttagggc caactaatac
     3541 tatgcctaaa atttcattaa ctatgaatgg acaagaaagt catgtttatc aattaaatgg
     3601 tgataggcac cgcatgcacg atgaatttgt tatttttgaa ggtattatta gcaatttaga
     3661 ttttaagcgt gctgctcaag ctttagaaca tagtagaacg gtgatgaaag ttctagatat
     3721 ggtaacgaaa taagtaaatg actcttttga gtcatttttt acttatttta aatactgtat
     3781 gttatactaa ttagtaataa aaccgaaagt gagataataa aatgatttca tcaattggac
     3841 aggtaatgct ctacgttagc aatgttgaag catcagcaga tttttggaaa aacaaggtgg
     3901 gttttgagcg tgttgaaaaa cagacacaag gagattatgt aacctatata gttgcaccga
     3961 agttggatag tgaagttagc ttcgtcttac atgacaaagc aattatagct caaatgagtc
     4021 ctgagctaga tttagcaact ccctctattt tatttgaaac tactgatatt gacagtacat
     4081 atcaagaatt aacagcaaat gaggttatga caaatcctat tgttgatatg ggagtatgag
     4141 agtttttaat ttttccgata atgacaataa ttactttgca atccgtgaag taaaataaaa
     4201 aggagaactt atggttaaag cactagaaac atacatcgta acaaatggaa atgggcgaca
     4261 agcggttgat ttttataaag atgtctttca agcggattta gtgaatatga tgacatggga
     4321 agagatggac cccaactgtc ttgaagaccg caaggattta atcattaatg ctcagcttat
     4381 ttttgatggc attcgtcttc aaatctcgga tgaaaatcca gatttcgtgt atcaagctgg
     4441 taaaaatgtt acagccgcta ttattgtggg tagcgtagag gaggcgcgtg aaatttatga
     4501 aaaattaaag aagagtgcac aagaagttca gttagagtta caagaaactt tctggagtcc
     4561 tgcttacgca aacttagtag accaatttgg tgtgatgtgg caaatttcaa cagagttaaa
     4621 ctaatcgttt ttaccatctg agcattgcaa tgatagattt ataaaaatac tgattgcaaa
     4681 cagagaagaa aaaattaacg gatttcatgt attgaatagc taccaaaagg tagctatttt
     4741 attcctaatt gaagtaagct attttagcta aagtctattg taatagggat ttaaaggggt
     4801 attagtgata agttgaaggc attatgatat aataggttga attatattca gagatagagg
     4861 aattaatatg tcaaattatg ccattatttt agctgcgggt aagggaactc gcatgaaatc
     4921 tgatttacca aaagttatgc ataaagtatc tggtattacc atgcttgagc atgttttccg
     4981 tagtgtgcaa gctattgagc caagtaaaat tgttactgtt attggacata aggcggagct
     5041 tgtacgtgat gttttaggtg acaaatcaga gtttgtaatg caaacagaac agctaggaac
     5101 tggccatgct gtaatgatgg cggaagaaga attagcgaca tcaaaaggac atacactagt
     5161 tattgcaggt gatacacccc tgattactgg agaaagttta aaaaacttaa ttgattttca
     5221 tgtcaatcat aaaaacgttg caacaatctt aacagccgat gctgcaaatc catttggata
     5281 tggtcgtatt atccgtaatt cagatgatga agttactaaa attgtagaac aaaaggatgc
     5341 taatgacttt gagcagcagg ttaaagagat taatactgga acttatgttt ttgataatca
     5401 aagtctattt gaagcactta aagacattaa tacaaataac gctcaaggag agtattatct
     5461 aactgatgtt attggaattt tcaaagaagc gggtaaaaag gtaggtgcct ataaacttcg
     5521 tgactttgac gaaagtctag gagttaatga tcgtgtagca cttgcaactg ctgaaaaagt
     5581 gatgcgtcac cgaattgcac gtcaacatat ggttaatggg gtcacagtgg ttaatcctga
     5641 ttccgcttat attgatattg atgttgagat tggagaagaa tctgtaattg agcctaatgt
     5701 gactttaaaa ggacagacga agattggtaa aggtacttta ttaaccaatg gtagttattt
     5761 agtagatgct caagtcggaa atgatgttac catcactaat tcgatggttg aagaatccat
     5821 tatttctgat ggagtaaccg taggccctta tgcgcatatt agaccaggaa caagtcttgc
     5881 aaaaggtgtt catattggta atttcgttga agtaaagggt tctcaaatag gtgagaatac
     5941 gaaggcaggt catttaactt atatcggcaa tgcagaggta ggttgtgatg ttaactttgg
     6001 cgcagggacg attactgtca attatgatgg acaaaataaa tttaaaacag aaatcggaag
     6061 taatgtcttt atcggaagta attctactct gattgcacca cttgaaattg gtgataatgc
     6121 tttaacagca gctggatcta ccatcacaga taatgttcca attgatagca tagctattgg
     6181 aagaggacgt caagtcaata aagaaggata tgccaataaa aaaccacatc atccttcaca
     6241 aaagtaacac ttatttggga ggttcttatg gattttgaag aaaaaacaat taatcgtcaa
     6301 actgtttttg atggacaaat tatcaaagta gctgttgatg atgtagagtt gccaaatgga
     6361 ttgggacaat ccaaacgtga acttgttttt catggaggag ctgttgctac tttggcggta
     6421 actcctgagc ataaaattgt tttagtgaaa cagtatcgta aggctattga aggtatttct
     6481 tacgaaattc cagcagggaa acttgagacg ggtgagtcag gttctaagga agaagctgcc
     6541 cttcgggaat tagaagaaga gactggctac acagggaatc ttgaaattct ttatagtttc
     6601 tacactgcta ttggtttttg taatgagaaa atcgtcctct atctagctac tgatttacaa
     6661 aaagttgaaa atcctaggcc acaagatgat gatgaggtcc ttgagcttct agagttaagt
     6721 tatgaggatt gtatgcaaat ggtagaaaaa ggcatgattc aagatgcgaa aacgatcatt
     6781 gctttacaat attacggttt aaaaatggga ggtcattctt aatgggaaaa ccattgttaa
     6841 cagatgatat gattgaacgt tcaaatcgag gtgaaaaagt ttcaggtcaa acaatcttag
     6901 atcaagaaac taaaattatt tctacagaag atggcatgga gcaactaact gacgagaacg
     6961 gtaaacatat ctacaagagt cgccggattg agaatgcaaa acgaaatgaa tttcaacgta
     7021 agttgaattt agttttattc attttgctta ttttgttggc tctcttgttt tatgcaattt
     7081 ttaaattata aggaaatatc atgaaaattg gaattattgc agccatggag gaagagttaa
     7141 aactccttgt ggaaaatctt gaggataaat ctcaagagac agtattatca aatgtatatt
     7201 acagtggacg ttatggtgaa catgagcttg ttttagtaca gtctggtgtt gggaaagtca
     7261 tgtcagcgat gagtgttgct attttagtag aaagctttaa agttgatgct attattaata
     7321 caggatcagc tggtgcagta gcaacaggat tgaatgtagg agacgtcgtt gtcgcagata
     7381 cattagttta tcacgatgta gatttaaccg cctttggtta tgactatgga caaatgtcaa
     7441 tgcagccact ttattttcat tcggataaga cttttgtttc cacttttgaa gcagttttat
     7501 ctaaagaaga aatgattagt aaagttggtt taattgcaac tggggatagt tttattgctg
     7561 gccaggaaaa gattgatgtt attaaggggc attttccaca ggttttagct gtcgagatgg
     7621 aaggtgcagc aattgcccaa gctgctcaag ctacaggaaa gccctttgtg gtggtacgtg
     7681 ctatgagtga tactgcagct catgatgcca atatcacatt tgatgaattt atcattgaag
     7741 ctggtaaacg ctctgcccaa gtattaatgg catttttgaa agcattataa aaaagcccgt
     7801 gtatctaatg aatacacgga cttttttatt tatgtagcag atacatatat ttgacttgcg
     7861 atacgctcag ggattgttag attttcatcg ttataggtga tgtgataagt ttttgcataa
     7921 gggtcaattt gtgtcaattc aatggtatcg ttgatatgca aacggtgttg ttctaggtat
     7981 ttcagtagct gaaaatcatc atgaacacga ctgataacat atgtgccaag ctgactaatt
     8041 tggtccaatg tcatttggtt aatttctact aaaaactctc cctttttagg aatagtccca
     8101 ccgtgtggac aaaattgtgg aaagtctaac aacttttcta attgatcaac aaaagttgtt
     8161 gatacagtat gttctaaaac ttccgcttct tcatgaatat catcagcagt atagtgcaaa
     8221 tggttaacta aaaatacctc tactagacga tgtttacggt aaaggtcaga aacaactaat
     8281 aaaccttgtt tggtaagata atatcctaaa tctttatctt taacaatcag ttgttcactt
     8341 atcatttttt taatcatttc tgacacggca ggagccgaga cttgcatcat ttcagcgatt
     8401 ttcttattgg aaattttgtt gtttttttca cctaattcat gaatgcattt taggtaatct
     8461 gctttatttg gagtcatatt cagtctttct tatacttctt aaaatctatt tttctaagac
     8521 cattatatca aaaaatactg aaaaagtatt tgacaaatac tttataatag gttatgctaa
     8581 tatcaaaatt aagttaactt aatttaatac ataacttata ttaattttaa aaggagcatt
     8641 ttgatgaaaa agtggttagt tattgtatca tgttttgtag catttttagg cttaggtgcc
     8701 tgtgctaaca agcaggatca agccaaaaac gaaaaattaa aagtagttgt aaccaactct
     8761 attttggcag atataacaaa aaatatagcc ggtaacaaaa tcgacttaca tagtattgta
     8821 cctgttggta aagatcctca tgaatatgag ccattaccag cagacgttga aaaaacaact
     8881 caagcagacc ttgtttttta caacggaatt aatcttgaaa caggcggtaa tgcttggttt
     8941 acaaaattaa taaagaacgc taagaagaaa gaaaacaaag attactttgc ggttagcgat
     9001 ggagttgatg ttatttatct aaatggtcaa agcggaaaag gtaaagaaga cccacatgct
     9061 tggttgaacc ttgaaaatgg tattatttac tcaaaaaata ttgctaagca attaattgct
     9121 aaagatccaa aaaacaaagc tacttatgag aaaaacagag atgcttatgt agctaaatta
     9181 gaaaaattgg ataaagaagc aaaatctaaa tttaatgcca ttccagctaa taaaaaatta
     9241 attgttacta gtgaaggttg ctttaaatat ttctcaaaag cttatggagt tccatcagca
     9301 tacatctggg aaattaacac tgaagaagaa ggtacaccag atcaaattac tagcttagtt
     9361 aagaaattaa aacaagtaag accttcagct ttattcgttg aatcaagtgt tgacaagcgt
     9421 cctatgaaat ctgtttcaag agaatcaggt atcccaattt acgcagaaat cttcacagac
     9481 tcaattgcta aaaaaggtca aaagggtgac agctactacg ctatgatgaa atggaatctt
     9541 gataagattg cagaaggttt agcaaaataa gagtgagaat cgacattgaa atcataatga
     9601 aatcaatgat gtagtattct cgcttaaggc tgagttgtaa caatgtcgaa atcctaatct
     9661 aaaaactgac ttagctttaa taaatacaga gaagtggagt ctggttttat aaccagctcc
     9721 attctacttt tggaggaaaa atgattatta gtaaacactt aagcgtaagt tatgataata
     9781 atttagtgct agaagatatt aatttacgct tagaaggctc cggaatcatt ggtattttag
     9841 ggcctaatgg ggcggggaaa tctacgctta tgaaagctct tcttggtttg gttgattcta
     9901 ctggagaatc agggattggg ggagatttgc tacctttaat ggggagagta gcatatgttg
     9961 aacaaaaaac taatattgat taccaattcc ctataactgt tggagaatgc gtttctttag
    10021 gattatataa ggaacgaggc ctcttcaagc gactatcaaa aactgactgg gaaaaagtat
    10081 cccgagtgat tgatcaagtt ggtttgagag ggtttgaaaa tagaccaatt aatgccttat
    10141 caggtggaca atttcaacgt atgcttatgg caagatgtct cgttcaagaa gcggattaca
    10201 tttttttaga tgagccgttt gtagggattg attccattag tgaacaaatc atagttaatt
    10261 tactaaaaaa attaagtaag gccggaaaat taattttagt cgtccatcac gatttgagta
    10321 aagttgatca ttattttgat caagtaatta ttttgaaccg tcatctaatt gcttgtggac
    10381 caatagatca ggcatttacc cgtgagaatt taagtgctgc ttacggtgat gctattttgt
    10441 taggacaagg agactagaat gtttacgaaa ttttttgaag gcttattgac ttatcatttt
    10501 ttgcaaaatg cttttataac tgcaattgtg attggtattg tagcgggtgc agtaggctgt
    10561 tttattatct tgcgctcaat gtctttgatg ggtgatgcta tttctcacgc tgttctacca
    10621 ggtgttgcta tatcttttat tttagggatt aactttttta taggtgctat tgtctttggt
    10681 ttgttgtctt cgattatcat tacttatatt aaagaaaata gtgtcataaa aggagatact
    10741 gctattggaa ttactttttc gtccttttta gccttaggta tcattttaat tggacttgct
    10801 aatagtacaa ctgacctctt tcatattctt tttgggaata ttttggcagt acaagatagt
    10861 gataaatata tgactattat tgtgggatta attgtactga ctctgattac tatcttcttt
    10921 aaagaattat tattgacttc ttttgatcct gttttagcaa aatcaatggg aatgagagtt
    10981 tctttttatc attatttatt gatgattttg ttaacattag ttgcagtaac agctatgcag
    11041 agtgtgggaa caatattgat cgtagcactt ttaattacac cagctgcaac agcatatctt
    11101 tatgttaaaa gtctcagaac aatgttgttt ttatcatctg cattaggagc agtagcatct
    11161 gttctggggt tatatatcgg ttatacattt aatattgccg caggatcaag tattgtttta
    11221 acatctactt ttatgttttt acttgctttc ctattttctc ctaaacaaag tttgtttaaa
    11281 aagaaacaat agtgttgtta aaaaatgaga ctagaaaatg ttctagtctc atttttgata
    11341 gaattattta aatttgtagt ctttaataac tttgatactt gtaatagtaa ttttttcttt
    11401 tggttgatct gattttgtaa cttctaccga agcaatttta tccacagttt ccatgccaga
    11461 gattacttgt ccaaagacgg tataaccacc atctaaactt ggatttcctc cctctgaata
    11521 ggctttaata atcaccttag gaactttttt atctgacagt tgtttggaat gatcttgctg
    11581 gctttgattg ataaagaatt ggctgccatt agtatcagct ccagcatttg ccattgctaa
    11641 acttcccctg atattgtaga ggtaagggga tatttcatta acaaaaccat ttccagaatc
    11701 tttcttttta tctttgctat tccatataga ttttccaccg gtcccgtctc cattagggtc
    11761 accagattgt atcataaaat ctttgataac tctgtggaaa cttaacccat tatagtatcc
    11821 ttcttttgca tgtgttagaa aattttcaac agctaaagga gcatattttg gaaataactt
    11881 gatatttata tcacctttag aggtttttat aagcacttgt gcctcgtttt tggcaacagc
    11941 cttgtccaat tgaggaaaag cttttttatc agcttttaag gcttgcttag tttttttgtt
    12001 tagttgttca gtagcttcct ttgaagagtt ttcagctagt ttttggtcaa cataacggtc
    12061 tccttttaga gaacgctcaa ttgactcgca accacttaaa gttaatatac tgacacaggc
    12121 taaaccaaga taaataattt ttttcataca tactccttga ttaagttatt aagatgatta
    12181 tattttatca taaattttta aataatttat aaaagtaaat agtaaaccta ttgggggact
    12241 tgttgaaatt atgatagaat agtatagcat gagttgatag agaaagtgag gaacaagtat
    12301 ttcacttgaa atatggtatt tatggcaaat aagaaaaaaa caaaaggaaa gaaaaccaga
    12361 agacctacta aggcagaaat agagcgtcaa agagctattc aaaggatgat tactgctctt
    12421 gttttaacaa ttattctctt ctttggtatt atcagattag gtatttttgg tattacagtc
    12481 tataacgtca tccgttttat ggtaggtagc ttggcttact tatttattgc ggcaacttta
    12541 atctaccttt atttctttaa atggttgcga aagaaagata gcttagtagc aggttttttg
    12601 atagcttctt taggattatt gattgagtgg catgcttacc ttttctcaat gcctattttg
    12661 aaagataaag aaattttgcg ttcaactgct cgattaattg tgtctgattt aatgcaattt
    12721 aaaatcactg tttttgccgg tggaggtatg ttgggtgctt tgatttacaa gccaattgct
    12781 tttctctttt ctaatattgg tgcctatatg attggtgttc tcttcatcat tttgggtctc
    12841 tttttaatga gttctctgga agtttatgac atcgtcgaat ttattagagc ttttaaaaat
    12901 aaagtggcag agaagcacga gcaaaataaa aaggagcgtt ttgctaagcg agagatgaaa
    12961 aaagcaatcg ctgaacaaga gcgcatagag cgtcaaaaag ctgaagaaga agcttattta
    13021 gcttcggtta atgtagaccc tgaaacgggt gagattctag aggatcaagc tgaggacaat
    13081 ttggatgatg cgctaccacc tgaggtaagt gaaacatcaa ctccggtatt tgagccagag
    13141 atccttgctt atgagacatc gcctcaaaat gatcctttac cagtagagcc gacaatttat
    13201 ttagaagact atgattcgcc gattcctaat atgagagaaa atgatgagga aatggtttat
    13261 gatttagatg atgatgtaga tgatagtgat atagaaaatg tcgactttac acctaaaacg
    13321 acactggttt ataaattacc aacgatagat ttatttgcac cagataagcc taaaaatcaa
    13381 tccaaagaaa aggatttagt ccgaaagaat atcagagttt tagaagaaac atttagaagt
    13441 tttggtatcg atgtaaaagt agaacgtgct gaaattggac catcagttac taaatatgaa
    13501 attaaaccag cagttggagt tcgtgtgaat cgtatttcaa atctatctga cgacctagct
    13561 cttgctcttg cagcaaaaga tgtgcgtata gaagcaccaa ttcctggaaa atcattaata
    13621 ggtattgaag ttcctaactc agaaattgca acggtttctt tccgcgaact ttgggaacaa
    13681 tctgatgcca atcctgaaaa ccttttagaa gtaccactag gaaaagctgt taacggcaat
    13741 gctcgcagtt ttaacttagc tagaatgccg catcttttgg tagctggttc aactggttca
    13801 ggtaaatctg tggcagttaa tggaattatt tcaagtattt tgatgaaggc acgtccagat
    13861 caagttaagt ttatgatgat tgatcccaaa atggttgaat tatctgttta taatgatatt
    13921 ccacatttat taatccctgt tgtaaccaat ccgcgtaaag caagtaaggc actccaaaaa
    13981 gttgttgatg aaatggaaaa tcgatacgag ttatttagca aaattggtgt gcgtaatata
    14041 gcaggttata atacaaaggt tgaagagttt aatgcttcct ctgagcaaaa acaaatacct
    14101 ttgcctttaa tcgttgtcat tgtagatgaa ttggctgact tgatgatggt tgctagtaaa
    14161 gaagttgaag atgctattat tcgtttgggg caaaaagcac gtgctgcagg tatccatatg
    14221 attcttgcaa ctcaacgtcc atccgtagat gttatttctg gtttgattaa agcaaatgtt
    14281 ccgtcgcgta ttgcatttgc tgtttcaagt ggtactgata gccgtacgat ccttgatgaa
    14341 aatggtgctg aaaagctctt gggacggggt gacatgctct ttaagcctat tgatgagaat
    14401 catccagtac gactacaagg ttcctttatt tcagatgatg atgttgaaag gatcgttggt
    14461 tttatcaaag accaagccga ggctgactat gatgatgcct ttgatcctgg agaagtatct
    14521 gaaacagata acggctctgg tggtggcggc ggagtacctg aaagtgatcc tctttttgaa
    14581 gaagccaagg gactcgtttt agagacgcaa aaagcaagtg cctcaatgat tcaacgccga
    14641 ttgtctgttg gtttcaatag agcaacaaga ctaatggaag aattagaagc agcgggggtt
    14701 attggtccag cagaaggaac caagccacga aaagttttaa tgactccaac tccgagtgaa
    14761 taagaaaaaa ctaatctgac aagaaattgt cggattagtt ttttatgtta taatgaagca
    14821 aatagatgaa gaagaaggtt acgaatgcat attgagactg ttattgattt caaagaatta
    14881 ggaaaaagat atcgttttaa aaatcctaca aaagaattaa tagctgatac tttagaacaa
    14941 gtcttagaag tgataaaaga agttgattat tatcaatctc aaaattatta tgttgttggt
    15001 tatttatctt atgaagcatc tgctgctttt gattcacatt ttaaagtttc tcaacagaag
    15061 ttggctggag aacatctagc ttattttaca gtacataaag attgtgagaa cgaagctttt
    15121 cctttaagtt atgaaaatgt tagattagca gataattgga ctgctaatgt ttctgagcaa
    15181 gaatatcaag aggcaattgc taatattaaa ggacaaatta gacaaggaaa tacttatcaa
    15241 gtaaattata cactagagct tagccaacaa ttatgctcgg atccctttag tgtttatgaa
    15301 cgcctaatgg tagaacaagg agcaggatat aatgcttata ttgcctacga cgataaaagg
    15361 attttatctg taagcccgga attgttcttt aagaagaagg atgaagtctt aacaactaga
    15421 ccaatgaagg gaacaagcgc tagaaagcct acttatcaag aagatgtggc ggagcgagat
    15481 tggttggcaa atgatcctaa aaatcgttct gaaaatatga tgattgttga tttattaaga
    15541 aacgatatgg gccgtatctg tgatgttgga acagttaagg taaaaaaact atgtcaagtg
    15601 gagcagtatg caactgtgtg gcaaatgaca tcaactattg aaggagtttt atcgccagaa
    15661 gtgacactta tgtctatttt tcaagctcta tatccttgtg gatctatcac tggagctcct
    15721 aagatatcaa caatggctat tattaatgag ttggaaaaac ggccaagagg tatttattgt
    15781 gggacgatag gactttgcat gccagacgga caagctattt ttaacgttcc tattcgcaca
    15841 gtacaaatga aaggtcaaca agcctattac ggcgttggtg gaggtattac gtgggagagc
    15901 cagacagatt ctgaatatga agaaacccgt caaaaatcag ctgttttaac acgtgtcaat
    15961 ccaaaatttc aattaataac tacaggaaga gtgactgaaa ataaacttct tttctctcaa
    16021 caacatgtcg agagattagt tgaatcagcg tcttattttg cttattcttt tgataaaagt
    16081 aaattcgaaa gggaactgaa aaaatacctt catcagctag atgagaagga ttaccgcttg
    16141 aaaataatgc ttgataaaac tggtaaggta acgtttgagg taaaacaact agtgaattta
    16201 tcaaaaaagt ttttaacggc agaggtggtc gtacaagact accctattaa attatccccg
    16261 tttacttatt ttaaaacttc ttatcgccca catattattg aaggtcagaa tgaaaagata
    16321 tttgtatctc ctgaggggtt gctattggaa acaagtattg ggaatattgt tttagaaaaa
    16381 aatggaaggt ttttaacccc agatttatca gaaggagggt tgaatgggat ttatcgtcgt
    16441 catctcctta aaaaccaaaa agtaattgaa gcaccactaa ctttaaaaga tttagaatca
    16501 gccgatgcta tatacgcctg taatgctgtt agagggcttt atcctctaaa cctaaagtaa
    16561 aaagagttgg aaattttgta ctgaccccca aaagttggac aatttatttt aagtgaagga
    16621 tttagttctg tattgcacag gactaagtcc ttttagtttt gctttgattc gcttgttgtt
    16681 gtagtaaaaa atgtaatctg tgatagcttg ctcaaggtca tcaagtgatt tgtaagactt
    16741 ctccaagcca tagaacatct cagattttag aatcccaaag aaagattcca tcataccgtt
    16801 gtcaggactg ttccccttcc gtgacataga aggacgaatg cctttgtcct ccagaaattg
    16861 atggtaggac ttgtgctggt actgccagcc ttggtcgctg tggagaatgg tttcgctgta
    16921 tgaagccgca ggaaaagccc tctcaagcat ggtttgaacc tgtttcaagt caggagaccg
    16981 agacagggta aaatcaatta tctcactgtt atagccatca agaacaggcg atagataaag
    17041 tttcccttca ggtaaagcaa actccgtcac atcggtatag cacttctcat agggcttcga
    17101 cccctcaaac tggcgttgaa tcaggttatc agccttctta ccaacctcac ctttgtaaga
    17161 ggcatacttg cgcttacgac gaatacgagc agtcaagccc atacttttca tcaagccttg
    17221 cacccttttg tggttgatga caaagccacg atttcgcagt tctaaataaa tccgacgata
    17281 gccatagttt cctctatgtt catcataaat ggattgaatg tcagatttga ttgctttgtc
    17341 tttatttggt ttattcagtt tctttagttg ataatagtaa gttgcacgag ctatcttagt
    17401 tatctcaagt aagaggtcta gtcggaatcc tcctgtgacc atttctctaa ttgtttctgc
    17461 cgttcgctct gtaaggcttc gtccctcaag cgcaaatccc tcaacttttt tagaaaggca
    17521 ttctccgttc tcaagcgctc attttcttcc tgaagtcgct cgagctctgt catttcttcc
    17581 caagttttct tacgcttacg tcccatttta ctcggtctcc ctcttgtttt ctcaacaata
    17641 gtataaccat ttttcttaaa ttgggaaagc caatttgtga gtatagaaca attagagaga
    17701 gcataatcaa gagaaacaga gagttgagaa caaccatgaa tcaagacttt atctatcatt
    17761 tcctgcttta attcaggtgg ataatactca tttctacctt tttcgacgat ttctacacca
    17821 taccgatcca tgagttttat catgtattta agattcgaga tacgaacatc atatcgttga
    17881 ctaatctgag accaggacat cccgatcttt cttaactcgt atatttctag tttatcttca
    17941 taacttaatt tcatcagaaa agcaccccaa tcgttagatt ttttgtctaa cttttggggt
    18001 gcagttcaat ttatccaact ctttttattg tactttgata aagatgataa ccaataatcc
    18061 taggtaaaaa aggaaagtta gaataaagcc agatctccaa aataatttta aaaaacgttt
    18121 ataagaaaaa agagtctttg ttcgtagaag ctttattgta attccgattg ccagtaatga
    18181 aatgagaatc agatagtaag gtaaaagatt ttgttttaac agactagatg atattaatgg
    18241 aattaaaaaa acaaaaatag gaaaagcaac atctggaaac ataatgttaa attttttaag
    18301 tttaaatatt ttgacaagaa cctgtgaaaa tatagggatt aaaacaagaa ataaagaagc
    18361 aataattttg tatatcatag gtctatttta taccgatttt ggtaagaagt aaactgattt
    18421 tcaatagaat tagcgaaact acttggttaa aaaagcttta taaagtagga gagcaattcc
    18481 agataattca acatcttttt tataaatcat aatgtactct gaaaataact tattcttatg
    18541 aaaatggtag acattgcact ctttatccaa atagcgatga atgagagttt gtggaacaaa
    18601 tgttgctccc atccctgaca aagataggct agctgctgta ggaacggtac tagttgtaat
    18661 catctgattt aatgacatgt gatgaagctc atagaattgt gcaattgttt tgccaattcc
    18721 gtactctaat ggtgttagga taagaggttc attgtctaga actgaaatag gatattcaaa
    18781 aggaataatc tgtccacgtt tttcagcgtt gtagagttgg ctggtggtag gtatcattaa
    18841 aaacaattcg tcgcggtaaa tagtggttga tgcaattcca tctttataaa ggataggagc
    18901 catcccaaca gctaggtcaa tatcaccact cgctaccaat tgttcagaaa tattttgatt
    18961 gttttcgagt aatttgacag aaatttctgg gtgctccaag ttaaatttgg gaagtacatt
    19021 tgccagaata gaacttgcta agccagagtt aatcccaagt gtgatttctc cttttttatc
    19081 agaacggatg agatatagtt ctttggccat ttgacgctca atcatatcaa tttcttttag
    19141 gtagaataga tatctctgtc cagcataagt taaaaaggtt tggtgtcctt ggctctggat
    19201 gagttttatt tctaactcat tttctaattg cttgatcact ttgcttaggt aaggttgaga
    19261 aataaaaagg tgtgcagcgg ctttagtgat gtttgagtat agctcgatac tctctagata
    19321 cattaatttt tgttgatttt tcaagacttt ataaccagtt tgatatcctt agattaataa
    19381 taagttatga tacgttatgg ctataagttt actatcattt tgagctaaaa tcaatctatt
    19441 taatttagta gtataacatg atatattcat aactatttag ttatagaaat atctaaaaaa
    19501 atttatttgt aaaaaattat tttcaaaagt agactatata agagcagatt gttagaataa
    19561 tagccttatt ttaactgttc atattttaag gaggaaagca aatggccaat gcatctttgc
    19621 gacatcaatt atttgaaaaa cttgaccaaa aatgtgatca aatggttgct attcgtcgct
    19681 atttacatga aaatcccgaa ttatcattta aagagacaaa aacagcagct tatatttcag
    19741 atttttataa aggtaaggac tgtcatgttc agacacaatt tggtggtatg aatggcgtcg
    19801 tagttgatat ctatggtgat aaagcgacag ataagcccat caaacacatt gctttgagag
    19861 ccgactttga tgctcttcca attcaggaag agacagggct ttcttttgct tcaaaaacag
    19921 cgggcgtcat gcatgcttgt ggacatgatg cccatacagc ttatttattg attttggctg
    19981 agagcttgat tgaattaaaa tcagaatttt caggtcatat ccgtatctta catcaaccgg
    20041 ctgaagaggt gccaccaggc ggagcaaaag caatgattga agcaggatgt ttagatggca
    20101 ttgatgctgt ccttggtatc catgttatgt caacgatgga agagggaacg gtacaatatc
    20161 acgcaggtcc cattcaaaca gggcgtgcca cttttaaagt tattttgcaa ggtaagggtg
    20221 gacatggttc catgccacat cgtgcaaatg atacaattgt tgcagcttct agttttgtta
    20281 tggcagcgca aacgattgtc agtcgtcgag ttaatccatt tgacacagca gtagttacta
    20341 tcggatcttt tgatggtaaa ggttctgcta atgtgatcaa agatagtgta accttagaag
    20401 gtgatgtgcg cgtgatgtct gaagagacac gaggtgttgt tgaggaggaa tttaagcgta
    20461 ttctagatgg aatagcacaa acttatggtg ttagttatca gttagactat cagaatgatt
    20521 atcctgtttt agttaataac agtgaagtga cacagaaagt tgcaaatagt ttaaaatctg
    20581 ttgctataaa agaaatttta gatgtcattg attgcgaccc tcaaacgccg tctgaagatt
    20641 ttgcctatta tgctcaaaca atcccagctt gcttcttcta tgttggagca catgaagagg
    20701 ggcaaccata ttacccacat catcatccaa aattccaaat tgcagaatca tcattgatgg
    20761 tatctgctaa atcaatggca acagcagcgc ttgctatgct tgtagaagga gaatagataa
    20821 catatgttaa cgacaaagga aaatttcaaa ggaaataaca aattattggt gggaattgta
    20881 ctcgctgtac tatcattttg gttatttgca cagtctattt taaacatggg accagatgtt
    20941 cagtcatcat taggtatttc atctggtgca atggatattg gtgtctcaag tacggcattg
    21001 ttttcaggtt tatttattgt tgtaactggt ggtttagcag ataaacttgg tcgagttaaa
    21061 tttactttta ttggtctttg cttaaatatt attggttcac tactaattgt tttagctaat
    21121 ggagcagtgc tatttattat gggacgtatt tttcaaggat tggcagcagc ttttattatg
    21181 ccatcaacaa tggccttggt taaaacgtac tatgatggta aagatagaca acgtgcagtt
    21241 agtttctggt caatcggatc gtggggtggc tcaggtctat gttcttattt tggtggagcg
    21301 gtggcaagta ctctaggttg gcgttatgtc tttatctttt caatcatcgc atcagtagtt
    21361 agttttctat taattttagg aaccccagaa agtaaaaatg ttggacaaaa aactcacttt
    21421 gattaccttg gtttaattat ttttattatt tcaatgttat cgttaaatat tgggatatct
    21481 atggctcagg aacatggatt gatgaacgtt attccattga gtctttttac agtgatgctt
    21541 attggatttg ttctgtttta ttatgttgaa acacgtaaat ctaatagttt tattgacttt
    21601 catctgtttg aaaatcgatt ttacttaggt gcgacaatat caaatttctt actgaatgca
    21661 gttgcaggaa ctctgattgt tattaatact tacatgcaac aaggtcgcca gctaacacca
    21721 aaggttgcag gtgaaatgtc tttaggctat ttggtttgtg tactaattgc tatccgagtt
    21781 ggtgagaaaa ttttacaacg ttttggtgcc cgtaagccaa tgcttttggg agctatgagt
    21841 acatttgttg ggatattttt aatgactttg gtcaatattc aaggaccttt gtatcttgtg
    21901 ttagtctttg ttggttatgc tttatttgga acaggcttgg ggatctatgc gacaccatca
    21961 acagatacag ctatttctag tattcctaat gaaaaagtag gttctgcatc tggaatttac
    22021 aaaatggcaa gttcactagg tggtgcaatt ggtgtagcaa cttcaattgc gatttatcat
    22081 gctttttctg gtaatgcaga ttttcacaaa gcagctttgt gtggattaat tttaaaccta
    22141 gttttttgta gtctatcgat cttatcaatt ctttttgtta tccctaagaa aattgaggat
    22201 aaataaataa caatacttga gattcaaaat ctcaagtatt gttgtaataa tgtaaaagga
    22261 attgggaaag aaatttaaaa ta
//
LOCUS       DATRMA010000028        21820 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.028, whole genome shotgun sequence.
ACCESSION   DATRMA010000028 DATRMA010000000
VERSION     DATRMA010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21820
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.028"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            complement(387..839)
                     /locus_tag="VBC28_001019"
     CDS             complement(387..839)
                     /locus_tag="VBC28_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComE"
                     /protein_id="HEO6815015.1"
                     /translation="MNRLSWEDYFMANAELISKRSTCDRAFVGAVLVKNNRIIATGYN
                     GGVSETDNCNEVGHYMEDGHCIRTVHAEMNALIQCAKEGISTNNTEIYVTHFPCINCT
                     KALLQAGVKKITYKANYRPHPFAIELMEAKGVAYVQHDVPEVTLGMEE"
     gene            complement(851..1918)
                     /locus_tag="VBC28_001020"
                     /pseudo
     CDS             complement(851..1918)
                     /locus_tag="VBC28_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000505344.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
     gene            complement(2018..2734)
                     /locus_tag="VBC28_001021"
     CDS             complement(2018..2734)
                     /locus_tag="VBC28_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000584725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HEO6815016.1"
                     /translation="MIILIIALATILTFVTWMLIRPKALRYILGLLSLALLFASVWML
                     TDHFINHTGMTIETKTKTREIYSAGDSKAPFGLMIYKEIGTHSSNNVLVYRTDLNQKE
                     AKAHFVPNTKEVTSAVKKSASYQLADVKKATVTTKTKRYVWKDSLSKLLYGFAGENGE
                     LISQKSVAKIPRKTWLVLTKEQSEHLESAAAKLKEQMTSQAKVNPVKAMEIKTLAKTN
                     PEAYAEMQVKALKEALNIKE"
     gene            complement(2736..4235)
                     /locus_tag="VBC28_001022"
     CDS             complement(2736..4235)
                     /locus_tag="VBC28_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEO6815017.1"
                     /translation="MTTKAKHDIHGKPYNRTAMITLLLIATFAGVLNQTSLGTAIPTL
                     MNSFNISLSTAQQATTWFLLANGIMIPVSAYLATRFSTKWLYVTSYVVLLIGLLMTTL
                     APTSNWNLFLVGRIIQAISVGISMPLMQVVMVNVFPPEQRGAAMGLNGLVVGLAPAIG
                     PTLAGWILKQEFHFAGHDLTWRAIFLLPLLILTVTTILSPFVLKDVVDNKSVKLEVPS
                     LILSIIGFGSFLWGFTNVATYGWGDIGYVISPIMVGIIFIALFIHRQLKLETPFLDIR
                     VFKNKQFSVTTAAIALSMMAMMGVEMMLPLYLQNVHGLSALDSGLALLPGALMMGIVS
                     PISGAVYDKVGARRMAMIGFTILGVATLPFVFLTTTTPDHFITLLYAVRMFGIAMVMM
                     PLTASAMSALPPHEAAHGTAANNTARQIASAVVVALLSSVAQNIITNNKPSKDLLTMN
                     PLKYANQMLNASLDGFHVSFAIGFVFAVLGLLVSLFLRKGKIIETEKEV"
     gene            complement(4571..7399)
                     /gene="uvrA"
                     /locus_tag="VBC28_001023"
     CDS             complement(4571..7399)
                     /gene="uvrA"
                     /locus_tag="VBC28_001023"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001932063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEO6815018.1"
                     /translation="MQDKLMIRGARAHNLKNISVDIPRDKLVVVTGLSGSGKSSLAFD
                     TIYAEGQRRYVESLSAYARQFLGNMEKPDVDSIDGLSPAISIDQKTTSKNPRSTVGTV
                     TEINDYLRLLYARVGTPYCINGHGAITASSVEQIVDKVLALPERTKMQILAPIIRRKK
                     GQHKSTFEKIQKDGYVRVRIDGDIHDVTEVPELSKSKMHNIDIVVDRLINKEGIRSRL
                     FDSVEAALRLSDGYVVIDTMDGNELLFSEHYSCPECGFTVPELEPRLFSFNAPFGSCP
                     TCDGLGIKLEVDIDLVIPDRSKTLREGALVPWNPISSNYYPTMLEQAMTQFGVDMDTP
                     FEKLSKAEQDLALYGSGEREFHFHYINDFGGERNIDLPFEGVVNNINRRYHETNSDYT
                     RNVMREYMNELKCNTCHGYRLNDQALCVRVGGEEGLNIGQGSDLSIADHLELLETLRL
                     SSNEQLIARPIIKEIHDRLSFLNNVGLNYLNLSRSAGTLSGGESQRIRLATQIGSNLS
                     GVLYVLDEPSIGLHQRDNDRLIDSLKKMRDLGNTLIVVEHDEDTMMAADWLIDVGPGA
                     GAFGGEIVASGTPKQVAKNTKSITGQYLSGKKVIPVPSERRVGNGRFLEIKGAAENNL
                     QNLDVKFPLGKFIAVTGVSGSGKSTLINSILKKAVAQKLNRNSDKPGKYVSLEGIEYV
                     DRLIDIDQSPIGRTPRSNPATYTGVFDDIRDLFAQTNEAKIRGYKKGRFSFNVKGGRC
                     ESCSGDGIIKIEMHFLPDVYVPCEVCHGTRYNSETLEVHYKEKNIAQILDMTVNDAVT
                     FFAAIPKIARKLQTIKDVGLGYVTLGQPATTLSGGEAQRMKLASELHKRSTGKSLYIL
                     DEPTTGLHADDIARLLKVLDRFVDDGNTVLVIEHNLDVIKTADHIIDLGPEGGIGGGQ
                     IVAIGTPEEVAENPKSYTGYYLKEKLAR"
     gene            complement(7531..8202)
                     /locus_tag="VBC28_001024"
     CDS             complement(7531..8202)
                     /locus_tag="VBC28_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 family protein"
                     /protein_id="HEO6815019.1"
                     /translation="MTLQDLTKKNQEFVHIATNQLLADGKSDAEIKAILEEHLPEIID
                     NQKKGITARSLLGAPTTWAASFTERPEDKARVSVQKNTNPWLMWLDTSLLFLGLVTAL
                     NGLMLLFGQSNVNTGLISILTLGFGGGAAMYVTYYYIYRHMGKPKSERPGWLKSFAVL
                     ALVMLVWFALFAVVPLLPATINPKLPEVVLFIIALASFGLRFYLQRKYNIQSSMAPVA
                     PQQRR"
     gene            complement(8227..9171)
                     /locus_tag="VBC28_001025"
     CDS             complement(8227..9171)
                     /locus_tag="VBC28_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003085337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEO6815020.1"
                     /translation="MKQMFLSTAIEFKEIETFEPGAWINLVNPSQEESEQVADQFGID
                     IDDLRAPLDVEETSRISVEDDYTLVIVDVPTYEERNNKSYYMTIPMGIIVTDNAVITT
                     CLEHLTLFDHFYRRRVKNFYTFMKTRFVFQLLYRNAELYLQALRTIDRQSDKIEAQLE
                     SATRNEQLIDMMELEKSIVYLKASLKFNERIVKKLTSSTSSLKKYIEDEDLLEDTLIE
                     TQQAIEMANIYENVLNAMTETTASIIGNNQNTIMKTLALVTMTLDIPTVIFSAYGMNF
                     QNNWMPLNGLAHGFIYVVLLAFLMSSFVVFYFIRKKWF"
     gene            complement(9341..9580)
                     /locus_tag="VBC28_001026"
     CDS             complement(9341..9580)
                     /locus_tag="VBC28_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEO6815021.1"
                     /translation="MAQQRRGGFKRRKKVDYIAANKIEYVDYKDTELLSRFVSERGKI
                     LPRRVTGTSAKNQRKVTTAIKRARVMALMPYVNED"
     gene            complement(9625..10116)
                     /locus_tag="VBC28_001027"
     CDS             complement(9625..10116)
                     /locus_tag="VBC28_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEO6815022.1"
                     /translation="MINNVVLVGRMTRDAELRYTPSNQAVATFSLAVNRNFKNQSGER
                     EADFINCVIWRQQAENLANWAKKGALVGITGRIQTRNYENQQGQRVYVTEVVAESFQL
                     LESRATREGGSPNSYNNGGYNNAPSNNSYSASSQQTPNFSRDESPFGNSNPMDISDDD
                     LPF"
     gene            complement(10128..10415)
                     /locus_tag="VBC28_001028"
     CDS             complement(10128..10415)
                     /locus_tag="VBC28_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018030687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEO6815023.1"
                     /translation="MAKYEILYIIRPNIEEEAKNALVARFDSILSDNGATVVESKDWE
                     KRRLAYEIQDFTEGLYHIVNVEAEDAVALNEFDRLSKINGDILRHMIVKVD"
     gene            11509..12633
                     /gene="mutY"
                     /locus_tag="VBC28_001029"
     CDS             11509..12633
                     /gene="mutY"
                     /locus_tag="VBC28_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEO6815024.1"
                     /translation="MWPEDRIASFRRTLLGWYDQEKRDLPWRRTTNPYYIWVSEIMLQ
                     QTQVNTVIPYYKRFLEWFPQIKDLADAPEEQLLKAWEGLGYYSRVRNMQKAAQQVMVD
                     FGGIFPHTYDDIASLKGIGPYTAGAIASISFNLPEPAVDGNVMRVMARLFEVNYDIGD
                     PKNRKIFQAIMEILIDPDRPGDFNQALMDLGTDIESAKTPRPDESPIRFFNAAYLNGT
                     YSKYPIKNPKKKPKPMRIQAFVIRNQNGQYLLEKNTKGRLLGGFWSFPIIETSPLSQQ
                     LDLFDDNQSNPIIWQTQNETFQREYQLKPQWTDNHFPNIKHTFSHQKWTIELIEGVVK
                     ATDLPNAPHLKWVAIEDFSLYPFATPQKKMLETYLKQKNA"
     gene            12810..13403
                     /locus_tag="VBC28_001030"
     CDS             12810..13403
                     /locus_tag="VBC28_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO6815025.1"
                     /translation="MEIGQQIIRYRKQQALSQEELAEKVYVSRQSISNWENDKTYPDI
                     HSLLLLSQIFQVSLDQLIKGDIEKMKYTITQVDKKNFERDTKVMVTLMILLMISSYPL
                     VYFLEWLGLGIFVLLSIITMTYANRVERFKKKYDVQTYKEILAVSSGKLLDEIEKREE
                     RAKLPYQKPLIVTVFFLITVATFFASRFIFTWLFHLS"
     gene            complement(13449..13763)
                     /gene="trxA"
                     /locus_tag="VBC28_001031"
     CDS             complement(13449..13763)
                     /gene="trxA"
                     /locus_tag="VBC28_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001162959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEO6815026.1"
                     /translation="MALEVTDATFVEETKEGLVLIDFWATWCGPCRMQAPILEQLSQE
                     IDEDELKILKMDVDENPETARQFGIMSIPTLMFKKDGEVVKQVAGVHTKDQLKAIIAE
                     LS"
     gene            complement(13844..14344)
                     /locus_tag="VBC28_001032"
     CDS             complement(13844..14344)
                     /locus_tag="VBC28_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEO6815027.1"
                     /translation="MESYEQFYAKLSQPFRKSPQLIILLNFLLKIVTGMMYILYPSFL
                     IFTLWQGMTFQLWLRLLIIPAVGFIALSYIRKRLDFPRPYEKWNIKPLIYKDTEGRSM
                     PSRHVFSATMISMCLLRYYVYFGIVCLILSVLLAICRVIAGIHYPKDVIVGYLIGLIL
                     GLCLFI"
     gene            complement(14348..16687)
                     /locus_tag="VBC28_001033"
     CDS             complement(14348..16687)
                     /locus_tag="VBC28_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HEO6815028.1"
                     /translation="MNNKILEQLEFNKVKELILPYLKTEQSQEELSELEPMTEAPKIE
                     KSFNEISDMEQIFVEHHSFGIVSLSSISESLKRLELSADLNIQELLAIKKVLQSSSDM
                     IHFYSDLDNVSFQSLDRLFENLEQFPNLQGSFQAINDGGFLEHFASPELERIRRQLTN
                     SERRVRQILQDMLKEKAELLSENLIASRSGRSVLPVKNTYRNRISGVVHDISSSGSTV
                     YIEPRAVVTLNEEITQLRADERHEESRILHAFSDLLRPHVATIRNNAWILGHLDFVRA
                     KYLFMSDNKATIPEISNDSTLALINVRHPLLSNPVANDLHFDQDLTAIVITGPNTGGK
                     TIMLKTLGLAQLMGQSGLPVLADKGSKIAVFNNIFADIGDEQSIEQSLSTFSSHMTHI
                     VSILNEADHNSLVLFDELGAGTDPQEGASLAMAILEHLRLSNIKTMATTHYPELKAYG
                     IETNFVENASMEFDAETLSPTYRFMQGVPGRSNAFEIASRLGLAPFIVKQAKQMTDSD
                     SDVNRIIEQLEAQTLETRRRLDHIKEVEQENLKFNRAVKKLYNEFSHERDKELEKIYQ
                     EAQEIVDMALNESDTILKKLNDKSQLKPHEIIDAKAQIKKLAPQVDLSKNKVLNKAKK
                     IKAARAPRIGDDIIVTSYGQRGTLTSQLKDGRWEAQVGIIKMTLTQDEFTLVRVQEEQ
                     KVKSKQINVVKKADSSGPRARLDLRGKRYEEAMQELDNFIDQALLNNMGQVDIIHGIG
                     TGVIREGVTKYLRRNKHVKHFAYAPQNAGGSGATIVTLG"
     gene            complement(16772..17314)
                     /locus_tag="VBC28_001034"
     CDS             complement(16772..17314)
                     /locus_tag="VBC28_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEO6815029.1"
                     /translation="MLSLLLLIIVIWHFYIGYSRGIFLQVFYVLMSMVSLMIASQFYQ
                     ELASQITLWVPYSNPVQGVEVYFFKDISKFQLSHVYYAGVAFVFIYSLSYLVGRLLGV
                     LLHLAPVEHFDSLQNNIISGFLAVLVCLLFMSMCLTILATVPMSFVQEKLWNSLFVRF
                     LINDLPFFSQFLVRTWAVGL"
     gene            complement(17317..17628)
                     /locus_tag="VBC28_001035"
     CDS             complement(17317..17628)
                     /locus_tag="VBC28_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000448289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6815030.1"
                     /translation="METKINNSQKNRYKFVFGDKPLTLTTDKDNLFMEEIERVATEKY
                     EAIKEKLPNADNETIAILMAINALSVQLSREIDIEKMEDELNKLRSKTISDIKEKVSE
                     D"
     gene            17739..18632
                     /locus_tag="VBC28_001036"
     CDS             17739..18632
                     /locus_tag="VBC28_001036"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HEO6815031.1"
                     /translation="MNTIVMQADKKLQEKIRTDLAQHHISNNNPYVVFSAKISGATVL
                     LYTSGKLVFQGSNASHIAQKYGFIEQKESCSSESQDIPIIGTDEVGNGSYFGGLAVVA
                     SFVTPKDHAYLKKLGVGDSKTLTDQKIKQIAPLLEKAIPHKALLLSPQKYNQVVSPNN
                     KHNAVSVKVALHNQAIFLLLQDGFEPEKIVIDAFTSSKNYQNYLKNEKNQFKQTITLE
                     EKAENKYLAVAVSSIIARNLFLENLNKLSDDVGYKLPSGAGHQSDKVASQLLKAYGIS
                     SLEHCAKLHFANTKKAQALLK"
     gene            18648..19241
                     /gene="lepB"
                     /locus_tag="VBC28_001037"
     CDS             18648..19241
                     /gene="lepB"
                     /locus_tag="VBC28_001037"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEO6815032.1"
                     /translation="MKEFIKEWGVFILILSLFLLSRIFLWQFVKVDGHSMDPTLADKE
                     QLVVLKQTKINRFDIVVANEEEGGQKKKIVKRVIGMPGDVIKYKNDTLTINNKKTEEP
                     YLKEYTKLFKKDKLQEKYSYNPLFQDLAQSSTAFTTDSNGSSEFTTVVPKGHYYLVGD
                     DRIVSKDSRAVGPFKKSTIVGEVKFRFWPIRRFGTIN"
     gene            19370..21790
                     /locus_tag="VBC28_001038"
     CDS             19370..21790
                     /locus_tag="VBC28_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000451606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HEO6815033.1"
                     /translation="MEVFFTGTIERIIFENASNFFKILLLEIEDTDSDFDDVEVIITG
                     TMADVIEGEEYTFWGTLTQHPKYGEQLQSVRYERAKPTSGGLVKYFSSEQFKGIGKKT
                     AQRIVELYGDNTIDKILESPEQLSTISGLSKINREAFIAKLKLNYGTEQVLAKLAEYG
                     LSNRAAIQIFDHYKEESLEVINENPYQLVEDIQGIGFKIADQLAEQVGIESDSPKRFR
                     AAIIHTLVESSMEQGDTYIEARTLLEKTITLLEEARQIELDPSIVAKELTNLIAEDKV
                     QHIGTKIFSNTLFFAEEGIKKNLQRILNQPLDKQLNHKDIDREIRDIQKSLNIHYDNI
                     QEKAIREALLSKVFILTGGPGTGKTTVINGIIEAYSELHHIDLNKNDIPIVLAAPTGR
                     AARRMNELTGLPSATIHRHLGLNGDSDYQSLDDYLDCSLIIIDEFSMVDTWLANQLFD
                     ALDSHTQVIIVGDSDQLPSVGPGQVLADLLNINALPHVKLEKIFRQSEESTIVTLANQ
                     MRQGFLPEDFTAKKADRSYFEASANIIPNMISKIVQSALKSGIEAHEIQILAPMYRGQ
                     AGINNLNLIMQNLLNPLKDNNQFTFNDINFRIGDKVLHLVNDTELNVFNGDIGYITDL
                     IPAKYTESKQDEIYMTFDGQEVIYQRKEWLKITLAYAMSIHKSQGSEFQVVILPITRQ
                     SGRMLQRNLIYTAITRSKSKLILLGEIGAFDFAVKNEGAKRNTYLIERFENKQEIANS
                     QKIEDSSIDQKIDNTIINTSIPKTATPIEQTNLSKITYRLTEENYLTIDPMIGINQQD
                     ISAIFDSK"
BASE COUNT         7180 a         4226 c         3501 g         6913 t
ORIGIN      
        1 tgatatcatc tttatagtta tcatacaccc aatcaaatgc catattagca taaactggat
       61 ttacatctat tgaggtcagc aacaatatct tttctctttt ttgtaaatta tttttgcaat
      121 tcgagattcc cagcagtact aattttagat gttgataata gatggatcag ctattctatt
      181 atcaacatct aaaactttct gtgaaaaatc attaggaata atgactatta actggtgatc
      241 acctttttta ggccattttc agtaatccca tgactaacaa ttgaccaatt ttgagaatta
      301 tctcgttcaa tatatttaac ataactggcc cttagcttaa acgataaaac cttcaaggaa
      361 gataggcata tactctatct tcctctttac tcttccatcc ctagtgttac ctctggaaca
      421 tcatgttgaa cataagcaac tccttttgct tccataagtt caatagcaaa tgggtgtggg
      481 cgataattag ctttataagt aattttctta accccggctt gcaagagcgc cttggtgcaa
      541 ttgatacaag gaaaatgcgt cacataaatt tctgtattat tagtagaaat accttcttta
      601 gcacactgaa taagggcatt catctcagca tgaacagtcc taatacagtg accatcttcc
      661 atataatgtc ccacttcgtt acagttatct gtctcagaga ctcctccgtt ataacctgta
      721 gcaatgatgc gattattttt gactaaaaca gctccaacaa aagcacggtc acaggtagaa
      781 cgtttagaaa tcaactctgc atttgccata aaataatctt cccaagaaag tctattcatt
      841 ctattttcct ttaaagtaca attaattctt taggtgcaga tgtcaatact tcgcaacctg
      901 tttccgttat taataaatca tcttcaatac gaacaccgta tttattatcg agatatattc
      961 ctggctcgtc agtcactacc atcccagatg caatcactcc ctctgactta ccaaaataag
     1021 gaatttcatg aacatcaagc cccataccat gccctatacc gtgggtaaaa tgattaccgt
     1081 atccagaatt ttcaatgact tgtcgcgcca agtaatcata atcacaacgc ttcattcctg
     1141 attttacatt accaataata gcttgattac ttttcaaaac gatatcgtaa atctcgcgtt
     1201 cctgatcagt gacatgaccg atatgaatcg tacgtgtcat atcactaaca tagtgctgat
     1261 agtaacaacc aaaatctaaa gtcagagttt ctcccgattg aatagtcttc tgactcgcaa
     1321 ccccatgtgg catcgctgat cgatatccag atgctacaat aaaatcaaat gaaggaccag
     1381 ttgctcctaa ttctctcatg cgaaagtcta aaaaattagc aacttgcaat tcagttgttc
     1441 gatcaggctt tataaaatcc aacacatcta aaaaagcttg gtccgaaata cgacaggctt
     1501 gacggatgat cgcaagttat gttacatctt taatcatacg taacttttca acaacagatg
     1561 gagcttctat caagcgataa tcttcaaaaa cttccaccat atgtttgtac atatgataag
     1621 aaatatcagt ttcaaaacca atcgctatca atgcgtcatc gcgaataatt ttcaaaatag
     1681 ccgcaagtgg ttctctgcta atgataatat caaactctct tacagtattc tttgctatca
     1741 aggcatatcg tgaatctgta acaaaaatcc ggtgttttgg actaatgagt accgttgcat
     1801 tagttcctga aaaaccagtc aaataaaaaa tatttgttaa atttgttatt aagagtgcgt
     1861 cacatgataa ttgtgaaatt gctgtttgac atctagttag acgttgttca aaacccataa
     1921 gaaacctcgc tattctaacc ttaattaggt taattttatc atcttttaaa acatttagct
     1981 ataagaaaag caactcattt cagttgcttt tactttacta ttcttttata ttaagagctt
     2041 cttttaatgc tttaacttgc atctcagcat aagcttctgg atttgtctta gctaatgtct
     2101 ttatctccat agctttcact gggtttactt ttgcttgcga agtcatttgt tcttttagtt
     2161 tagccgcagc cgattctaag tgttcggact gctctttagt caaaactagc caagtttttc
     2221 gtggtatttt agcaactgat ttctgagaaa tcaattctcc attttcacct gcaaagccat
     2281 aaagtagttt cgaaagacta tctttccaca cataacgttt agtctttgtt gttactgtgg
     2341 cttttttcac atcagctaat tggtagctag cagatttttt cacagcactt gtcacttctt
     2401 ttgtatttgg aacaaagtga gcttttgctt ctttttgatt taaatctgtt cgataaacta
     2461 aaacattatt actcgaatga gtaccaatct ctttataaat catgagacca aatggagcct
     2521 tactatcacc cgcactataa atctcacgtg tctttgtctt agtttcaata gtcatacctg
     2581 tatgattaat aaagtgatcc gttaacatcc aaactgaggc aaacaacaaa gctaatgaga
     2641 gaagacctag aatataccgt agagcttttg gacgaatcaa catccatgtg acaaatgtca
     2701 aaatagttgc cagtgcaata atcaaaataa tcatgctata cctccttttc cgtctcaata
     2761 attttccctt ttctcaggaa aagagatacc agtaaaccaa gaaccgcaaa tacaaaacca
     2821 atggcaaaag aaacatggaa gccatcaaga ctagcattca acatctgatt tgcatatttt
     2881 aaaggattca ttgttagcaa atcttttgat ggtttattgt tggtgataat attttgagca
     2941 acacttgaaa gcaatgcaac tacaactgct gaagcaattt gacgagcagt attattagct
     3001 gctgttccat gtgcggcctc atgtggtgga agtgcgctca tcgcacttgc tgtcaatggc
     3061 atcataacca tagcaatacc aaacatacgt actgcatata agagggtaat aaaatgatct
     3121 ggtgttgtag tcgttaagaa aacaaaaggt aacgtcgcta cacctagtat ggtaaaacca
     3181 atcatagcca tacgtcttgc accaacttta tcatatacag ctccagaaat tggactaact
     3241 atccccatca tcaaggcacc tggtaataaa gctaaaccag aatcaagtgc agagagacca
     3301 tgaacatttt gcaagtaaag cggtagcatc atttcaacac ccatcatcgc catcattgaa
     3361 agtgcaatag ctgctgttgt tactgagaat tgtttatttt tgaaaacacg gatatctaag
     3421 aacggtgttt ctagttttaa ttgacgatga atgaataagg cgataaaaat aataccaacc
     3481 ataataggag aaataacata tccaatatct ccccatccat aagttgccac atttgtgaag
     3541 ccccacaaaa agctaccaaa gccaattatt gaaaggataa gggaaggcac ttccaattta
     3601 actgacttat tatcaaccac atcttttaga acaaaggggg ataaaattgt agtgaccgtt
     3661 aaaattagta aaggaagaag gaaaattgca cgccatgtta aatcatgccc tgcaaaatga
     3721 aattcttgtt ttaaaatcca ccctgctaat gtaggcccaa tagctggggc aagaccaacc
     3781 accaaaccat taagtcccat agcggcacca cgctgctctg gaggaaaaac attcaccata
     3841 acaacctgca tcaaaggcat agaaatccct acagaaatcg cttgaataat acgtcctact
     3901 aaaaaaaggt tccagttaga tgtaggtgct aacgtcgtca ttaacagtcc aataagtaaa
     3961 acaacgtagg atgtcacata caaccatttt gttgaaaaac gtgtcgctaa ataagctgaa
     4021 acaggaatca taatcccatt cgctaataaa aaccatgttg ttgcttgttg agccgttgac
     4081 aacgaaatgt taaagctatt catcaaagtt gggatagccg ttcccaaact tgtctgattt
     4141 aatacccctg caaatgtagc aatcaacaat aaggtgatca tggctgtacg attatatggt
     4201 ttgccatgaa tatcatgttt tgcttttgta gtcattttat ctccaatctc tatttctgat
     4261 tttaactaat atcttttaag aataacaaat aggtcttata aggttatttt ttgatttttt
     4321 aatcaagaaa taatattatt ttctgaaaaa atcattattt tagttatcgt gagaactatt
     4381 ttataatagc taaattttat tgtcaagaaa aagattctca tagaaactat ttttttcaaa
     4441 acatcattat ttcctttttt taaaatatct ccccactatt tttcctaata aagcgttctc
     4501 catttaatga cttctacgca atgttagctt acaaaaagga actgagtaat attactcagc
     4561 tcccaataaa ctatcttgct aacttctctt ttaaataata tcctgtatag gatttaggat
     4621 tttcagcaac ttcttctggt gttccgatag ctacaatttg tccgccacca atacctcctt
     4681 cagggccaag atcaataatg tggtcagcag ttttaataac atcaaggtta tgctcaataa
     4741 caagcactgt attaccatca tctacaaaac gatccaaaac cttaagtaaa cgagcaatat
     4801 catctgcatg aagccccgtt gttggttcgt ctaatatgta aagactctta cctgtcgaac
     4861 gcttatgtaa ttcacttgcc agcttcattc gctgagcttc tcctcctgat aaggttgtag
     4921 ctggttgccc caaagtgaca tatcccaaac caacatcctt aatagtctgt aacttacgag
     4981 caattttagg aatagctgca aaaaatgtaa ctgcatcatt cactgtcata tcaagaattt
     5041 gtgcaatatt tttttctttg taatgtactt ccagagtctc actattatag cgcgtgccgt
     5101 ggcatacttc acaaggaacg tagacatctg gtaaaaaatg catctcaatt ttgatgatac
     5161 catcgccaga acaagactca caacgtccac ctttgacgtt aaatgagaag cgtccttttt
     5221 tataacctcg aatctttgcc tcattagttt gagcaaaaag atcacgaata tcatcaaaaa
     5281 cacctgtata ggttgcggga ttagaacgtg gagtccgacc aattggactt tgatcaatat
     5341 caatcaatcg atcaacatat tcaatgcctt ctaaagaaac atatttacct ggtttatctg
     5401 agttacggtt aagcttctgc gcaacagctt ttttcaaaat actattaatt aaggttgatt
     5461 ttccagaacc tgagacacca gttacggcaa taaatttacc gagagggaac ttgacatcta
     5521 aattttgtag gttgttttct gccgcacctt tgatttctaa aaagcgacca ttacctactc
     5581 ttcgttcaga tggcacagga atcactttct tacctgacaa atattgtcct gtaatagatt
     5641 tagtattttt agctacttgc ttcggtgtac cagaggccac aatctcacca ccaaacgcac
     5701 cagcgccagg tcccacatcg attaaccaat cagctgccat cattgtatct tcatcatgtt
     5761 caacaacaat aagcgtatta cccaaatcac gcattttctt aagactatca atcaaacggt
     5821 cattgtctcg ctgatgaagt ccaatggacg gctcatcaag tacataaagg acacctgata
     5881 aattagaccc aatttgagta gccaaacgaa tacgttgact ctcgcccccg gaaagtgttc
     5941 ctgcagaacg tgacagattt aaataattaa gtccaacatt atttaagaaa cttagacggt
     6001 catggatttc tttgataatt ggtcgagcaa ttaattgttc atttgaagat aaacgcaatg
     6061 tttctaaaag ttctaaatgg tctgctatcg ataaatctga gccttgccca atattaagac
     6121 cttcttctcc cccaactctt acgcaaagtg cttgatcatt gagacgatag ccatgacaag
     6181 tgttacattt tagctcgttc atatattctc gcataacatt gcgtgtgtaa tcgctattag
     6241 tttcatggta acgacgatta atattattga caaccccttc aaaaggcaaa tcaatatttc
     6301 tctcgcctcc aaaatcgtta atataatgga aatggaactc tcgttcgcct gaaccatata
     6361 gggctaggtc ctgctccgcc ttgcttaatt tttcaaatgg cgtatccata tcaacaccaa
     6421 attgagtcat agcttgctct agcatcgttg gataataatt tgatgaaatt ggattccaag
     6481 gaactaaggc cccttctcta agggtcttac ttctatctgg aataaccaaa tctatatcta
     6541 cttctaactt aattcctaag ccatcacagg tcggacaaga accaaaggga gcattaaaag
     6601 aaaaaaggcg tggctctaat tcaggaacag taaaaccaca ttcaggacaa gaataatgtt
     6661 ctgaaaacaa taactcattg ccatccattg tatcaataac gacataacca tccgacaaac
     6721 gtagagccgc ttcaacggaa tcaaataagc gggaacgaat gccctcttta ttaattaatc
     6781 gatctactac aatatcaata ttgtgcatct tactttttga taattcaggc acctcagtaa
     6841 catcgtgaat atcaccatca atacgaacac gaacgtaacc atctttttgt attttctcaa
     6901 atgttgattt gtgttgtcct ttttttcgac gtataattgg ggctaaaatt tgcatctttg
     6961 ttcgctctgg aagcgctagt accttatcca caatttgctc tacagaagat gctgtgatgg
     7021 ctccgtgacc attgatacaa tatggggttc caacacgagc ataaagcaaa cgtaaataat
     7081 cgttaatttc tgttacagtc ccaacagtag agcgaggatt cttacttgtt gttttctgat
     7141 caatagaaat agctggacta aggccatcaa tagaatctac atcaggcttt tccatatttc
     7201 ccaaaaattg gcgcgcgtaa gcagaaagac tttcaacgta acgacgttgt ccttctgcat
     7261 agatagtgtc aaaggccaaa ctagacttac ctgatcctga tagtcccgta acgacaacta
     7321 acttgtctcg tggaatgtcg acacttatat tttttaaatt gtgagctctt gctcctctta
     7381 tcattaattt atcttgcata atgttgtgcc tcagcactcc tttttgaata gtgttgactt
     7441 attatatcaa attttaataa agtcagtatt tttttagttt taaatttcat agaaaaaaga
     7501 gatgaactaa tacgttcata ctctttgtta ttatcttctt tgttgtggtg ccactggtgc
     7561 cattgagctt tgaatattat atttacgttg taaataaaag cgcaaaccaa acgatgctaa
     7621 agcaataata aacaaaacaa cctcaggaag tttaggatta atagttgcag gtagaagagg
     7681 tactactgca aataaggcaa accaaactaa cataactagt gccagtactg cgaaagattt
     7741 aagccagcct ggtctttcag attttggttt acccatatgg cgataaatat agtaataagt
     7801 aacgtacatg gccgcaccac caccaaaacc aagagttaaa attgaaataa gccctgtatt
     7861 gacattagat tgaccaaata gcaacattaa accattcaaa gcagttacta agccaagaaa
     7921 tagtaaggaa gtatctaacc acatcaacca aggattggta tttttttgta cagatactct
     7981 agctttatcc tcaggtcgct cagtgaatga ggctgcccaa gtagttggtg ctcctaagag
     8041 tgagcgtgcg gtaattcctt ttttctgatt atctataatc tctgggagat gttcttctaa
     8101 aatagctttg atttctgcat ctgattttcc atcagctaaa agttgattag ttgcaatatg
     8161 aacaaactct tgattctttt tagttaagtc ttgtaaagtc atgtgaactc cttgattaat
     8221 atactattaa aaccattttt tacgaataaa ataaaataca acaaatgaac tcatcaaaaa
     8281 ggccaaaaga acaacataga taaagccatg tgcgagacca ttcaaaggca tccaattatt
     8341 ttgaaagttc atcccatacg ccgaaaaaat aaccgttgga atatccaatg tcatagttac
     8401 tagggctaag gttttcataa tggtattctg attattaccg atgattgaag cagtcgtttc
     8461 tgtcatggca tttaagacat tttcatagat atttgccatt tcaatagcct gctgagtttc
     8521 gatcaatgta tcttcaagca gatcttcatc ttcaatgtat ttttttaaac tactggtact
     8581 actggtcaat tttttgacga ttcgctcatt aaattttaaa gaggccttta gatagacaat
     8641 agatttttcc aattccatca tatcgatcaa ttgctcattt cgagttgcac tctctagttg
     8701 tgcttcaatt ttatcgctct gcctatcgat agtacgcaaa gcctgtaagt ataattcggc
     8761 attgcgataa agcaattgga aaacaaaacg tgtcttcata aaggtataaa aatttttgac
     8821 acgtcgtcta taaaagtgat caaacagcgt caaatgttct aggcaagtag taataacagc
     8881 gttgtcagta acgataatgc ccataggaat agtcatataa taacttttgt tattgcgttc
     8941 ttcataggta ggaacgtcaa caataactaa agtataatca tcttcaaccg agatacggga
     9001 agtttcttcc acatcgaggg gagcacgtaa atcatcaata tcaataccaa attgatcagc
     9061 tacttgttcg gattcttcct gggagggatt aaccagattt atccaagcac ctggctcgaa
     9121 tgtttcaatt tctttaaatt caattgccgt tgaaagaaac atttgcttca tggcgattcc
     9181 tcctttgtta taaaaaatcc ataccttaat attatacagg aaaaccattt gttttgcacg
     9241 gttaatacgg tggtatccta aatttttcta ttatttgact cgtaaatacc aataataatg
     9301 gtaaaaagaa taaaaaaata gccgctaggg ctattttata ttaatcttca tttacgtaag
     9361 gcataagagc cataacacga gcacgtttga ttgctgttgt tactttacgt tggtttttag
     9421 ctgaagttcc tgttacacga cgtggtaaaa tttttccacg ttctgaaacg aaacggctaa
     9481 gaagctctgt atctttgtaa tcaacatatt caatcttgtt agctgcgata taatcaactt
     9541 ttttacggcg tttgaatccg ccacgacgtt gttgagccat gtttatttct cctttatagt
     9601 tttaaattct gtccatctat tatattagaa tggtagatca tcatctgaaa tatccattgg
     9661 atttgagtta ccaaacggac tctcatcacg actaaaatta ggtgtttgtt gagaagaagc
     9721 tgaataacta ttatttgacg gagcattgtt atagccaccg ttattataag agttaggtga
     9781 accaccttct cgtgtagcac gactttccaa taattggaaa ctttccgcaa caacttctgt
     9841 tacatagaca cgttgacctt gttggttttc ataattacgc gtttggatac gacctgtaat
     9901 tccaaccaaa gcaccttttt ttgcccagtt agccaagttt tcagcttgtt ggcgccaaat
     9961 aacacagtta ataaaatcag cctcacgttc gccagattga tttttaaaat tacgattaac
    10021 tgcaagtgaa aaagtggcta ccgcttgatt acttggtgta taacgaagtt ctgcatcacg
    10081 ggtcatgcga cctacaagta ctacattatt aatcataaac taccttctta gtctacttta
    10141 acgatcatgt gacgaagaat gtcaccgttg atttttgaaa gacgatcaaa ctcattaaga
    10201 gctactgcat cttcagcttc aacgttaacg atatggtaaa gtccttcagt gaaatcttgg
    10261 atttcgtatg caagacgacg tttttcccaa tcttttgatt caacaacagt tgcaccgttg
    10321 tcagataaga tagaatcaaa acgtgctacc aaagcgtttt tagcttcttc ttcaatgttt
    10381 ggacgaataa tataaagaat ttcgtattta gccattgata tgttcctcct tttggtctaa
    10441 tgactcatga atttttcatg agtaagtgag gttatgctca caagatatta ttatagtaga
    10501 aataagttaa taatgcaagg tttttatgtt tttatttccc tgatatcatt taaagacatt
    10561 ttttattttt ctttattgaa atatgcctta tatcaaacct atgtagccat tttcttttac
    10621 tgacttatgt tcaatcccca ttctataatt caattaaaaa ataaaaaggc gaggtttaca
    10681 cctcaccttc aacatattat ttaaagaatt ctttattggt ttaagttttc gttggagaaa
    10741 aattcagtgt ctccgatttt atatttttaa atattaaaat actatttagg gagtccctca
    10801 ttatgtttta ttacctttgg taacaccaaa acatacacga acttttattc atatgtgtta
    10861 ccattttaca tctttcatat tgagatattt gattagcaat acctccttga ctaacaaaaa
    10921 ttactaacac atctaggggt ataccctagt ttatatttgt tcatgattac ctcaagcaag
    10981 catgcgctaa tggtaatgaa ctattaccac attgtctgtc ttccttaaat tatataagga
    11041 cttatgtgtc accgttactg tagcgaccta catctaaacc taacataagt gcccctccaa
    11101 ctatttctag gaaatgaaga aaacttcaaa tccttgtgac tgtttgcaac atcaatgcat
    11161 tgataacaat tttagtgtat cgccattctt atagcgaccc atattaaaaa ttaacataag
    11221 cgctacctcc attaactaaa gttagcttgc ctaccaatct ttcttttgga gactagtctc
    11281 cattcttata cctagagata ttgtaataga tcatggataa cctccttgat ttaaacttta
    11341 tctttataaa ttaattatag tgtaaacgct tccttaagtc aagaaaaaaa tattgatgca
    11401 acatttttct tcatcattaa gtacgatcta gtcaacatac ctctttgtca aatcctcttt
    11461 ctttgataca attaatctat gatagattta aaagactatg gaattgagat gtggcctgaa
    11521 gatagaatcg cttcttttcg tcgcacactt ttagggtggt atgaccaaga aaaacgtgat
    11581 ctcccttggc gaagaactac taatccatac tatatttggg tttctgaaat catgttgcaa
    11641 caaacacaag ttaatacagt aatcccttat tataaacgtt ttctagagtg gtttcctcaa
    11701 ataaaagatt tggcagacgc tcctgaagaa caattactaa aggcatggga gggactaggc
    11761 tattattctc gggtgcgtaa tatgcaaaag gctgcacagc aagtgatggt tgattttggt
    11821 ggcatatttc ctcacactta tgatgatatt gcttcattaa aagggattgg tccttatact
    11881 gcaggtgcta tcgccagcat ctcattcaac ttaccagagc ctgctgttga tggcaatgtg
    11941 atgcgtgtca tggctcgttt gtttgaagtt aattatgaca tcggtgaccc taaaaatcgt
    12001 aaaatttttc aagctattat ggaaatacta attgaccctg acagacccgg tgattttaac
    12061 caagcactta tggatctggg tacagacatc gaatccgcta agacacctag acctgatgaa
    12121 agccctattc gtttctttaa tgctgcttat ttgaatggta cttatagtaa atatcccata
    12181 aaaaatccta agaaaaagcc taaaccaatg cgaattcagg cttttgtcat tcgtaaccaa
    12241 aatggtcaat atctactgga gaaaaatact aaagggagac ttctaggggg tttttggtca
    12301 tttcctatta tcgaaacgtc acctctttct caacaattag atttatttga tgacaatcaa
    12361 tccaacccca taatctggca aacccaaaat gaaactttcc aaagagaata tcaattaaag
    12421 ccccaatgga ctgataatca ctttccaaat attaagcaca cttttagtca ccagaagtgg
    12481 acaatagaac tcatagaggg ggtggtcaaa gcaacagatt tacctaatgc tcctcaccta
    12541 aagtgggtag caattgagga cttttctctg tatccctttg caacaccaca aaagaaaatg
    12601 ctagagacct acttaaaaca gaaaaatgct taatcacaag tgaactatga cggttcactt
    12661 tttgtttaca attcctttac gccgctgtaa agttttcctc agattatctt ttaataccct
    12721 ataaatagtg gtctatccct aatgtcaaat acatttgata gacccgttag cttatcatcc
    12781 ttatactaat attatgaaag gagatattta tggaaattgg tcagcaaatt attcgctacc
    12841 gtaaacaaca agcgctctcg caagaagaat tagccgagaa agtctatgta agccgtcaga
    12901 gcatatctaa ctgggaaaat gacaaaactt acccagacat ccatagcctc ttactcctaa
    12961 gtcaaatttt tcaagtgagc ttagaccaac taattaaagg agacattgag aaaatgaaat
    13021 ataccatcac tcaggttgat aaaaagaatt ttgaacgaga taccaaagta atggtgacat
    13081 tgatgatact attaatgatt tcaagttatc cattagtcta tttcttagag tggctaggac
    13141 ttggcatttt tgttttatta tcgataatta ctatgactta tgctaatcgt gtggaacgtt
    13201 ttaagaaaaa atatgatgtg caaacttata aggaaatcct agctgtttca agtggaaaat
    13261 tattagacga aatcgaaaaa cgggaagaaa gagcaaaact tccttatcag aaacccctta
    13321 ttgtcactgt atttttcctc attactgttg ccaccttttt tgcaagtcgt tttattttca
    13381 catggttgtt ccatttaagt tagtagataa taaaataaag ctcagcgtga cactgggctt
    13441 tattttattt aactcaattc agcaataata gctttcagtt ggtctttagt atgaacacct
    13501 gcaacttgtt taaccacttc tccgtctttt ttaaacatca atgttggaat tgacataata
    13561 ccaaactgac gggcagtttc cggattctca tcaacatcca ttttcaaaat tttgagttca
    13621 tcttcatcga tttcttgaga taactgttct aagattggtg cttgcatacg acatggacca
    13681 caccaagttg cccaaaaatc aatgagtact aaaccttctt tagtttcttc tacaaatgta
    13741 gcatctgtta cttctaatgc catctttttt ctccatttca tcttaatgat aagcctattg
    13801 tacaagaatc tagttgtttt tgaaactatt tgctacgaac tcgctaaata aataaacaaa
    13861 gccctaaaat caagccaata aggtaaccta caataacatc cttaggatag tgaatacctg
    13921 ctatcacacg gcaaattgcc aatagaaccg ataatattaa acaaactatt ccaaagtaaa
    13981 cataatatcg taacaaacac atactaatca tcgtagctga aaaaacatgt ctgctaggca
    14041 ttgaccttcc ctcagtatcc ttataaatta aaggtttaat attccatttt tcatatggtc
    14101 gtggaaaatc aaggcgtttc ctaatgtaag acaaagctat aaagccaaca gcagggataa
    14161 ttaatagcct taaccataat tgaaatgtca tcccttgcca aagagtaaat atcagaaaag
    14221 atggatagag tatgtacatc attcctgtga ctatttttag taaaaaattt aaaagaatta
    14281 ttaactgtgg ggattttcta aaaggttgag atagtttggc ataaaattgt tcatatgatt
    14341 ccataattta ccctaacgtt acaattgtgg cgccagatcc ccctgcattt tgtggggcat
    14401 aagcaaaatg cttaacgtgc ttattacgac gaagatattt tgtcactccc tcacggataa
    14461 cgcctgtacc aataccatga atgatatcaa cttgtcccat attgttaagc aatgcttgat
    14521 caataaaatt atctaactct tgcatagctt cttcgtatct tttacctcta agatcaagtc
    14581 gagctcttgg tccagaacta tcagcctttt taaccacatt aatctgttta cttttgactt
    14641 tctgttcttc ttggactcta acaagggtaa attcatcttg tgttaatgtc attttgataa
    14701 ttcccacttg tgcttcccaa cgtccatctt ttaattgact agttaaggta cctcgctgtc
    14761 catagctagt cactataata tcatcaccaa ttctaggagc acgagctgct ttaatttttt
    14821 tagccttatt taagacttta ttttttgata aatcaacttg aggtgctaat ttttttattt
    14881 gtgccttagc atctataatt tcgtgaggtt ttaattggct cttatcattg agttttttta
    14941 agatagtatc actctcattc aaagccatat ctacaatttc ttgagcttct tgatagattt
    15001 tttctaactc tttatcgcgc tcatgtgaaa attcattata gagtttctta accgcacgat
    15061 tgaatttgag gttttcttgt tcaacttctt taatatgatc cagtcttcta cgtgtctcaa
    15121 gtgtctgtgc ctctaactgt tcaataatac ggttaacatc tgagtcagaa tctgtcatct
    15181 gcttagcttg tttaacaata aatggagcta aaccaaggcg agaagcaatt tcaaatgcat
    15241 ttgatcgtcc aggaactcct tgcataaagc gatacgtagg gctaagcgtt tcggcatcaa
    15301 attccatgct cgcattctct acaaaatttg tctcaatccc ataagctttt aattctggat
    15361 agtgcgtggt cgccatcgtt ttgatattac ttaacctaag gtgttctaaa atagccatag
    15421 ccaaactagc accttcttga ggatccgttc ctgctcccag ttcatcaaag aggactaaac
    15481 tattgtggtc agcctcgttt aaaatactga ctatgtgcgt catatgacta gaaaaagttg
    15541 atagactttg ttcaatagat tgctcatcgc caatatctgc aaagatattg ttaaatactg
    15601 caattttact acctttatcc gctaatactg gcaaaccaga ctgtcccatt aattgtgcta
    15661 aaccgagtgt ttttagcata atcgtcttac caccagtatt gggaccagtg atgacaattg
    15721 cagttaaatc ttgatcaaaa tgtaagtcat tagccacagg gttacttaac agaggatgac
    15781 gaacattgat taatgctaac gtgctgtcat tagaaatctc aggtatcgtc gccttattat
    15841 cagacataaa aagatattta gcccttacaa aatcaagatg cccaagaatc catgcattat
    15901 ttctaatagt ggcgacatgg ggtcttaaca agtctgaaaa tgcgtgtaaa atacgacttt
    15961 cttcatgacg ttcgtcagct ctaagctgcg ttatctcttc gtttagtgta actacagcac
    16021 gaggctcaat ataaacagta cttcctgaag aagagatgtc atgaaccaca ccagaaatac
    16081 gattccgata agtatttttt actggtagga cacttcgtcc actacgacta gcgattagat
    16141 tctctgataa aagctctgct ttttccttaa gcatatcctg taaaatctga cgaacccgtc
    16201 gttcactgtt tgttaattga cgacggatac gctctaattc tggactcgca aaatgttcta
    16261 aaaaaccacc atcattgata gcttgaaaag acccttgcag attagggaat tgttccaaat
    16321 tttcaaacaa acgatccaaa gattggaaag aaacattatc caaatcagaa taaaagtgaa
    16381 tcatatccga agaactctgt aaaacttttt tgatagccaa aagttcttga atattaagat
    16441 cagctgaaag ctctaagcgt tttaaactct cagagattga acttaggctg actatgccaa
    16501 atgagtgatg ttcaacaaaa atctgttcca tgtcagaaat ttcattaaaa cttttttcta
    16561 ttttaggagc ctccgtcatc ggctccagct ctgataattc ttcttgtgat tgttctgtct
    16621 tgagataagg taatatcaat tccttaactt tgttaaattc taactgttct aaaatcttgt
    16681 tattcatact cttcattata cctaaattta tcacaaaatg acaacgttaa aaaggttgga
    16741 actcttactc caaccttact aaacattata ttcaaagacc taccgcccaa gtccttacca
    16801 aaaactgtga gaagaatggt aagtcgttaa ttaaaaatct aacaaacaga ctattccata
    16861 acttttcttg aacaaatgac atagggactg ttgccagaat agtcaaacac atactcataa
    16921 ataagagaca aaccagaacg gctaaaaaac cactaataat attattttgc aagctatcaa
    16981 aatgttcaac tggtgctaaa tgtagtaaaa cacctaacaa gcgacctact aagtaagata
    17041 aactgtagat aaaaacaaag gcaacacctg cataataaac atgactcaat tgaaatttgc
    17101 taatatcttt aaaaaagtaa acttctactc cttgtacagg attagaatac ggtacccata
    17161 acgtaatctg actagctaat tcttggtaaa attgactagc aatcatcaaa gataccattg
    17221 acattaacac gtaaaagact tgtaaaaaaa tacctctact gtagccaata taaaaatgcc
    17281 aaataacaat aatcagcaaa agcaatgata gcatgattaa tcctcactca ctttttcttt
    17341 aatgtccgaa atcgttttgg agcgtaactt attgagctca tcttccattt tttcaatgtc
    17401 aatttctcga cttagttgca ctgataaagc attaattgcc atgagaatag caatcgtttc
    17461 attatcagca ttaggtaatt tttctttaat agcttcatac ttttctgtag ctacacgctc
    17521 aatctcttcc ataaaaaggt tgtctttatc tgtcgtcaat gtgagaggct tatctccaaa
    17581 gacaaattta taacgatttt tctgactatt atttattttt gtttccatac tatacctctg
    17641 ctagcattat accgtaaaat agggctttcg taaaattcta ccccttatca ttacttgtct
    17701 attcccttat tcctcttttt tatgatagaa tattgtctat gaacacaatc gtcatgcaag
    17761 cagataaaaa attgcaagaa aagataagaa ccgatttagc tcaacatcat ataagtaata
    17821 acaatcctta tgtggtattt tcagctaaaa tatcaggagc tactgttctt ttatatactt
    17881 ctggaaaact tgtcttccag ggttctaacg cttcacatat cgcccaaaag tatggtttta
    17941 ttgagcaaaa agaaagttgt tcctctgaga gtcaagatat tcccataatt ggtactgatg
    18001 aagtgggaaa tggctcttac tttggtggac tggcagtggt agctagtttt gtaactccta
    18061 aggaccatgc ttacctcaaa aaattaggtg ttggtgattc aaaaacactt actgatcaaa
    18121 aaattaagca aatcgcgcct cttttggaaa aagcaattcc acacaaagca ttattgttat
    18181 cgcctcaaaa atataatcaa gtggttagtc caaataacaa acataatgct gtctccgtaa
    18241 aagttgcttt acacaatcaa gctatcttcc ttcttcttca agatggtttt gaaccagaaa
    18301 aaatcgttat tgatgctttt actagctcca aaaactatca aaattatttg aaaaatgaaa
    18361 agaatcaatt taaacaaacg attactctgg aagaaaaagc tgaaaataaa tatctagctg
    18421 tagcggtcag ttctattatt gcacgtaatc tattcttaga aaaccttaac aaattaagcg
    18481 acgacgtagg atataaactt ccaagtggag ctggtcacca atctgataag gtcgctagcc
    18541 aattactcaa agcttatggt atctcttcgc ttgaacactg cgcaaaactt cattttgcca
    18601 acaccaaaaa agcacaagca ttattaaaat aaatagcagg gaaaattatg aaagaattta
    18661 ttaaagaatg gggtgtcttt atcctcatcc tctcactttt tttactatcg cgtatctttt
    18721 tatggcaatt cgttaaagtt gacggacact ccatggatcc aactttagct gacaaggaac
    18781 agctagtagt tctcaaacaa acaaaaatca atcgattcga tattgtagtg gctaacgaag
    18841 aagaaggcgg ccaaaagaaa aaaattgtta aacgtgtcat tggtatgcca ggtgatgtca
    18901 tcaaatataa aaatgacacc ttaactatta acaataaaaa aacagaagaa ccttacctca
    18961 aggaatatac taaattattt aaaaaggata aattacagga aaaatattcg tataacccac
    19021 ttttccaaga cctagcacaa agctctaccg ctttcaccac tgacagcaat ggcagcagcg
    19081 aatttactac tgtcgtgcct aaaggccact actatcttgt tggtgatgac cgaattgtct
    19141 ctaaagatag tcgtgccgtc ggtcccttca aaaaatcaac gattgtggga gaggttaaat
    19201 tccgcttctg gccaattcgt cgttttggaa ctatcaacta aaggaaatta aaaattcacc
    19261 tgacaaaagt tgaaatagca tattatattg aaaataggga gtgacaaatc catttaaaat
    19321 agggatttac aatcactccc tttaactgtt tattaaaaag gaaggtacaa tggaagtatt
    19381 ttttacagga accattgaac gcattatatt tgaaaatgcc agcaattttt ttaaaatatt
    19441 acttctggaa atcgaagata cggatagcga ttttgatgat gtagaagtaa ttattactgg
    19501 tactatggcc gacgttatag agggagaaga atatactttt tggggaacac taacccaaca
    19561 tccaaaatac ggcgagcaac tacaaagtgt acgctatgaa agagccaagc ctacttcagg
    19621 tggccttgtg aaatactttt ctagcgaaca atttaaagga attggtaaaa agactgccca
    19681 acgcatcgta gaactctatg gagataatac cattgataaa atccttgaat cccctgaaca
    19741 gttaagtaca atctcaggac tatcaaaaat taatagagaa gcattcatcg ctaaactgaa
    19801 actcaattat ggaacagagc aagttttagc taaactcgct gaatatgggt taagcaatcg
    19861 tgctgctatt caaatatttg atcactataa agaagaatca cttgaagtga taaacgaaaa
    19921 tccttatcaa cttgttgagg atatccaagg tatcggtttt aaaatagctg accaacttgc
    19981 tgaacaagtc ggtattgaaa gtgattctcc taaacgcttt cgtgctgcta ttattcatac
    20041 tttagttgag tcttctatgg agcaaggaga tacctatatt gaggctcgta ctctcttgga
    20101 aaaaaccatt actctccttg aagaagctcg tcaaatcgaa ctagatcctt caattgttgc
    20161 taaggaattg acaaatttaa ttgcagaaga taaagtgcaa catattggaa ctaaaatttt
    20221 tagtaatact ctattttttg ccgaagaagg catcaaaaaa aacttacaac gaatactaaa
    20281 tcagcctctt gataaacaac taaatcacaa agatattgac cgtgaaatca gagatattca
    20341 aaaatcttta aatatccact acgataatat ccaagaaaaa gctattagag aagctttact
    20401 aagtaaggta tttatcttga ctggtggccc aggaacaggt aaaacaacag ttataaatgg
    20461 tattattgaa gcctattccg agttacatca tattgattta aacaaaaatg atattcctat
    20521 tgtacttgct gctcctacag gtcgtgctgc tagacgcatg aatgaactta ccggtttacc
    20581 gagtgctact attcataggc atttgggatt aaacggagat agtgactatc aaagtctaga
    20641 tgactatcta gattgttcct taattatcat tgatgaattt tcaatggttg acacttggct
    20701 tgctaatcaa ttatttgacg ctttggactc gcacacacaa gttattattg tcggagatag
    20761 tgatcaatta ccgtctgtag gtccaggaca agttctagca gatcttttaa atataaatgc
    20821 acttccacac gttaaacttg aaaagatttt tagacaatca gaagaatcaa ctattgtgac
    20881 tttagctaat caaatgagac agggcttttt gccagaggac tttacagcta aaaaagccga
    20941 tcgctcatac tttgaagcat ctgctaatat catcccaaat atgatttcta aaattgttca
    21001 atctgcactt aagagcggta ttgaagctca tgaaattcaa attttagctc ctatgtatcg
    21061 cggccaagca ggtattaata atcttaacct tatcatgcaa aatctcctca atccattaaa
    21121 agataataac caatttactt ttaatgatat aaatttccga ataggagata aagtgttaca
    21181 tttagtcaac gatacagagc ttaatgtttt taatggggat attggttaca taacggacct
    21241 tataccagcc aaatatactg agagcaagca agatgagatt tatatgactt ttgacggtca
    21301 agaagttatc tatcaacgca aagaatggct taaaataaca ctagcctatg ctatgagtat
    21361 tcataaatca cagggttctg aatttcaagt tgttattctg ccaataacga gacagagtgg
    21421 tcgaatgtta cagcgaaacc ttatttatac agctattacg agatctaaaa gtaaactaat
    21481 acttttagga gaaattggtg catttgattt cgctgttaaa aacgaggggg caaagcgcaa
    21541 tacttatctg atagaaagat ttgaaaacaa gcaagaaata gctaattctc aaaaaattga
    21601 agatagctca atagatcaaa agattgacaa cacaataatt aatacgtcca ttccaaaaac
    21661 agcaacacca attgagcaga ctaatctttc aaaaataact tatcgcctaa ctgaagaaaa
    21721 ttatttaaca atcgatccaa tgattggtat taatcaacaa gatataagtg caatttttga
    21781 tagtaaataa attttttcat aaaatcttga aaactcttcc
//
LOCUS       DATRMA010000029        21322 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.029, whole genome shotgun sequence.
ACCESSION   DATRMA010000029 DATRMA010000000
VERSION     DATRMA010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21322
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.029"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            complement(<1..213)
                     /locus_tag="VBC28_001039"
     CDS             complement(<1..213)
                     /locus_tag="VBC28_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014713948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6815034.1"
                     /translation="MINRQAKKFFDYYLKMVATEEYQSDYQRHEQEGLEFHKKYIEEK
                     HPEWRTRTDYFDEFSIGEFIILDTLSN"
     gene            complement(292..1788)
                     /locus_tag="VBC28_001040"
     CDS             complement(292..1788)
                     /locus_tag="VBC28_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEO6815035.1"
                     /translation="MKVGKTTRVLTVYQWKPDPSKKGNVLVKFAMRYTHPLITKSRYK
                     YLTRGENKGWYTTKAIPTGKDSKGHEKLLISDIKNSQLITKVSKILNEMIDETVLDLT
                     GEKPKKLEPSKTLCLKEIARPYDENNELYGLAFKWWVNRVKPAKNTLKTRVSVYNQHI
                     SPYFNENMSLKQFCMEQEKMQAIINNTSEGTSKNIHIYLKMIFDWAVEQGELTALQHP
                     ILNKRVKRKTLTSAEEQAKLREDIAEKYLEVDEANQVFKIIENWTKRHNNELVADVLR
                     IIYLTGMRPSEALGLNEDVLDFKNKLIKIHWQRASHNKTDKEMAEEGLRDEKERYRAI
                     LKTKESVRTIPMSPKVEQILLKYIERNKFQARFNPTYHDMGYIFTRVYIKGKNQQGSP
                     LYHTEISMFLRGGTSQSAKYNKKSGKSYKDIDDLVDFGRPVHIVPHMFRHSFVSVMAD
                     KNVSLNVIREFVGHSEDSREIEKIYLHVMQKGKHKIEQAMIDLAKIIK"
     gene            complement(1804..2052)
                     /locus_tag="VBC28_001041"
     CDS             complement(1804..2052)
                     /locus_tag="VBC28_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018164767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6815036.1"
                     /translation="MIELKTKSDYDLTKNWYRKKEFLDELWKGMKLPTLDHYIRQMRN
                     SPYSFGICGTHGNVFIHAEVFKDWFDYKIFHENEAVIA"
     gene            complement(2086..3129)
                     /locus_tag="VBC28_001042"
     CDS             complement(2086..3129)
                     /locus_tag="VBC28_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEO6815037.1"
                     /translation="MNTVRIDYFAVTIKNVLPERVLTDILLIPLEDFTLNNWGINKYQ
                     RHYSCSEIKVYFNDDRISMGVFIELKGQGCRQYEEFLDGNENNWIALISRLYQYTVNF
                     TRLDIAHDIFDGSLDVQRIYDYCKKGLCISKAKHFEYHEKSILENGERVGETIAIGSR
                     GNQQWCIYNKRMEQLGKQEVVESLSWIRAELRCWQGKANIIAEQLFLKRPLSSIYFEA
                     VNGHYRFIRPNETDSNRWRRKKVKWWIDYLKTENQTVLSITRAKTTLRQSEAWTEKQV
                     AKTLAKVYIAKYQAYDVQKAEDYIQSLLKEGLSRLTDNDEKDIEQYIREQNSSQFWGQ
                     KRRLENKSPLKIN"
     gene            complement(3134..3553)
                     /locus_tag="VBC28_001043"
     CDS             complement(3134..3553)
                     /locus_tag="VBC28_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002922924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6815038.1"
                     /translation="MVYNMTDVNIKIGTLDLDKAQFDIPKKVVLLSAKINYKYNEING
                     EKVKTEEISKITCTVLDADKVKVLTEMGISTDDLKAITLELIGNLDKISKLAQNESLL
                     NVPVELIQPKVRLAWNIVRSNWAGVKIVCEDIKVLGE"
     gene            complement(3819..5192)
                     /locus_tag="VBC28_001044"
     CDS             complement(3819..5192)
                     /locus_tag="VBC28_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002922922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HEO6815039.1"
                     /translation="MNIFNRFSFWNKQRVRNLKPILYLPFRYGKKVSIYFYVIFGILT
                     ISIIANFQTNLANTFVATIFLGITIVVFRFWRKRQKKYFNGIEYALNYMVVSNGFFES
                     KVVNGKEEIMNHISLQYQENTEDIRVACMKYGSKYDSKANQLSSLLSSALSLELGEMI
                     EYTNSVMYIFPKFAEKRLTWEDALQTKKLTISEKVGWTFGSPPHILLAGSTKSGKTVM
                     IENLVAQYLHLGSEIRLLDPKKGELSWVVGKKLEARLGYKVVYNSPFQIAGALREAVE
                     EMNRRFQIMADNPDTYISKGKVLSWADVKGNYPLVIVLDEGIAFKTEAETTKEGKQAY
                     QEAMSNLGSLLVKSRQASIEVIVGLQRASSDFIPTYMRQNFGTALLLGSTTADSDSCR
                     MMFSSQDIDYKTCDIGTGYIQIDGVIPTPKYVEVPFKSDELDFEKYFDKACDFYWSMR
                     NNDGLSD"
     gene            complement(5206..5427)
                     /locus_tag="VBC28_001045"
     CDS             complement(5206..5427)
                     /locus_tag="VBC28_001045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6815040.1"
                     /translation="MNKQYYFWVSSTFILLAIISVFAGFTAKGIDFTAFTPWLSIKCA
                     LALSLMVLFIQLPAIIIMFIDKKRSKNLE"
     gene            complement(5479..5811)
                     /locus_tag="VBC28_001046"
     CDS             complement(5479..5811)
                     /locus_tag="VBC28_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6815041.1"
                     /translation="MSKASYTSYPSHVRSSVESMNQVFNTPAKRKFVANKARLNLSDK
                     TKSDVYSEAYAYLLNSLGVITNIQDEFTQKLVAFYFEGQKRYCNGTSMLDDFIKYGKL
                     PHHFSSYK"
     gene            complement(5967..6326)
                     /locus_tag="VBC28_001047"
     CDS             complement(5967..6326)
                     /locus_tag="VBC28_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6815042.1"
                     /translation="MRLATESKLILDELQRQIFEEIGLEASYSSIVSQAIRKTVPKMH
                     EIDWQSLKKRNLALSSPKESNDWDYQTSFMLEKDVLDLISELQNYFLEVFQAKRIHRA
                     FCVRLCLKFHYLLLTNK"
     gene            6830..7501
                     /locus_tag="VBC28_001048"
     CDS             6830..7501
                     /locus_tag="VBC28_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000186297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEO6815043.1"
                     /translation="MTNYKNNFKKFMSNKLYVGLASITLLGIGGAIGVSGAALAHNHH
                     SEHMSFEHKHEGHDKHHDHREKYSLKTLKNISTKIDYKTALETAKKEARSGFISQIEL
                     NTKGDTPFYEIKVIDNKIAKKYKIDANSGKILSTTSDNQLNSKDAQLKQPSKDIQTAL
                     QAIQAKYKNAKIKEISLANNKSNKLVYKVKLLDGNTPKIAQFDSDKASFIKANKLNTT
                     HKNHD"
     gene            7586..8257
                     /locus_tag="VBC28_001049"
     CDS             7586..8257
                     /locus_tag="VBC28_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO6815044.1"
                     /translation="MKILVVEDEFDLNRSIVKLLKKQHYSVDSASNGEEALQFVSVAE
                     YDVIILDVMMPKMDGFTFLKLLRNKGSQVSILMLTARDAVEDRIAGLDFGADDYLVKP
                     FEFGELMARIRAMLRRANRQVSSDDIQIQDITINLSTKQVWRNDNLIDLTAKEYEVLE
                     YLARHRDQVLSRHQIREHVWDYDYYGESNIIDVLIKNLRRKLDNNRDGSLIKTKRGLG
                     YVIPK"
     gene            8241..9605
                     /locus_tag="VBC28_001050"
     CDS             8241..9605
                     /locus_tag="VBC28_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000898851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEO6815045.1"
                     /translation="MLFQSKINYPKKSSIITQITLWYSSFIFILVIGVLIGSFFISKS
                     IAENKSKKNLEAKAVQMSQALAKGHRYEAFEDGIFYSVYDQNGKVIYSGFPKGFKRDL
                     DHQHKHKKKLSLFSMENRTFQYVDIPISGKNQWLRAIRTVDRLDKQLTELLFSLGIVL
                     PLMLIIITVGGYLILKRTFRPIQEITETAQFITQNEDYTKRIITKNNENELTELAAVI
                     NTMLASIESSFVREKQFNNDVSHELRTPVTVILSESEYGKNYAENLSEAKESFEVIHR
                     QSLSMKKLVEQLLELTKAENPLSIQLEPLNFSIMMKQLVSDSSRLLDNTPIHLDSQIE
                     DDLWIIGQQTLLKRLFDNLFSNAIKFTNNHISISLRQSDNQIVFSIKDNGLGISVDDQ
                     SKIWNRFYQVDSARTKDSQSGIGLGLSLVKQIATIHRAKIWVDSKPDDGSQFTLTFPK
                     LKKD"
     gene            complement(9715..11268)
                     /locus_tag="VBC28_001051"
     CDS             complement(9715..11268)
                     /locus_tag="VBC28_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HEO6815046.1"
                     /translation="MPEKMTNSENKRSKGFRMPGAFTILFILTIFSVLATWWIPAGSY
                     SKLQFDTASSKLVVTDPNGKTVHVPATQTQLDKMNVKIKIKEFTSGAISKPVSVPNTY
                     KRLKQNPAGIGSVTTSMVNGTIEAVDIMVFIMVLGGMIGVVRKSGAFESGLLALTKKT
                     KGREFLLIFLVSLLMVLGGTLCGIEEEAVAFYPILVPIFLAMGYDSIICVGAIFLASS
                     VGTSFSTINPFSSVIASNAAGISFTEGLSWRTAGCIAGAIFVVVYLHWYAKKIKANPE
                     FSYSYEDRVEFNAKWGMTTNHTPSLFTIRQKIILSLFVISFPLMVWGVMSQGWWFPTM
                     ASSFLAITIIIMFLTATGANGIGERDVVDEFVNGASSLVGVSLIIGLARGINIILSQG
                     YISDTMLYTASKLASHVSGSVFIIVMMFIYFVLGFVVPSSSGLAVLSMPILAPLADTV
                     GIPRSVVVMAYQFGQYAMLFLAPTGLVMATLQMLDMKYSHWLKFVWPVVLFLLIFGGG
                     LLVLQVLIL"
     gene            complement(11362..12288)
                     /gene="arcC"
                     /locus_tag="VBC28_001052"
     CDS             complement(11362..12288)
                     /gene="arcC"
                     /locus_tag="VBC28_001052"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEO6815047.1"
                     /translation="MAKIVVALGGNALGNSPEEQLRLVKHTAKSLVALIKKGHEIVVS
                     HGNGPQVGAINLGMNFAAESGQGTNFPFPECGAMSQGYIGYHLQQSLLNELRQEGINK
                     EVATIITQIEVDESDQAFSAPTKPIGTFYDKETSEKIAIEKGYTFVEDAGRGYRRVVA
                     SPEPKKIIEINSIKTLIENDTLVIAGGGGGIPVINKGGYEGIAAVIDKDKSSALLAGE
                     LAADQLIILTAVDYVYTQFGKENQKALTEVNENQMIDYVNQGEFAKGSMLPKVIACMS
                     FLDHNPKGTALITSLNGLEDALDGKLGTRITK"
     gene            complement(12300..13298)
                     /gene="argF"
                     /locus_tag="VBC28_001053"
     CDS             complement(12300..13298)
                     /gene="argF"
                     /locus_tag="VBC28_001053"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003045350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEO6815048.1"
                     /translation="MKNLRNRSFLTLLDFSTAEVEFLLKLSEDLKRAKYAGIEQQKLV
                     GKNIALIFEKDSTRTRCAFEVAAHDQGAHVTYLGPTGSQMGKKETSKDTARVLGGMYD
                     GIEYRGFSQETVETLAEFSGVPVWNGLTDADHPTQVLADFLTAKECLHKPYKDIRFTY
                     VGDGRNNVANALMIGASIVGMTYHLVCPKELEPDPELLSKCQEIAKTTGASIEITADI
                     AEGVRDSDVLYTDVWVSMGEPDEVWKERIALLEPYRITQEMLNMTENPNVIFEHCLPS
                     FHNIDTKVGYDIYEKYGLKEMEVSDEVFEGPHSVVFQEAENRMHTIKAVMVATLGD"
     gene            13401..14696
                     /locus_tag="VBC28_001054"
     CDS             13401..14696
                     /locus_tag="VBC28_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HEO6815049.1"
                     /translation="MLDILSEKHLERRQRIIISAIAIALAAQINISILADGFIMTLSL
                     FILPVFLYFNDDINPILLCLGITFASPIFRGIILSIAGEAEIHQIIEFVLTDMAFYIC
                     YGITFYTIYWHRSYRNKGTFFFSIIICDYFANLVEISFLIKFNNYTITIFATLFAIAL
                     LRAFISCAVAYTYSYLSLLLQKDDHERRYYYFMWSTSAVKSEVYFMQKNIIEIENIMK
                     NAYLLDKELSKYHLPKEYQHLSLDISRDVHEVKKDYQNIIKGLGTYFSVKNESTMALK
                     DIFQIVLSYTRSIIQFRHQDIIILENNKCNLIISNYYYLLTIISNIVLNAVEAIDKQK
                     KGTISVHTEELEDFIKIEISDNGPGIPDKMKHMIFKPGFSTKFDANGDIYRGIGLSHV
                     RILMEEQYQGTITVCPNQPNGTTFTLLFRKNRFKLGDNT"
     gene            14693..15526
                     /locus_tag="VBC28_001055"
     CDS             14693..15526
                     /locus_tag="VBC28_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000675243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding domain-containing protein"
                     /protein_id="HEO6815050.1"
                     /translation="MKFYIIDDDPTITMILQDIIEEDFNNTVVRVNNVSSKAYNELLI
                     ADVDIVLIDLLMPILDGVTLVQKIYKQRSDLKFIMISQVKDNDLRQEAYKAGIEFFIN
                     KPINIIEVKSVVKRVTDTIEMQKKLNTIQNLLENTPSYQKPITTSNLTKIRSILSYLG
                     ITSETAYTDILNICELLLKQELNFAQFDFQKELSIDEHQQKIILQRIRRAVKKAMINM
                     AHLYIDDFENELTLQYANALFGFQNIHNEAQLIQGKSMYGGKISLKHFFDELILQSKT
                     F"
     gene            15748..16471
                     /locus_tag="VBC28_001056"
                     /pseudo
     CDS             15748..16471
                     /locus_tag="VBC28_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767600.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            16474..17988
                     /locus_tag="VBC28_001057"
     CDS             16474..17988
                     /locus_tag="VBC28_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019319700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HEO6815051.1"
                     /translation="MTTLITTFQERFGDWTQSLIEHLQLSLLTLILATLIAIPLGIII
                     SHYKKISHVVLQITGIFQTIPSLALLGLFIPFMGIGTVPAVVALIIYALFPILQNTVT
                     VLMQIDANLIEAATAFGMTRWERLKKFELALSMPVIISGIRTASVMIIGTATLASLIG
                     AGGLGSFILLGIDRNNPSLILIGAISSAVLAIIFSGLIGLLEKARLRTIAVSGILLLA
                     GLGLSYAPKWMPGTNTATITVAGKLGTEPDILINMYKELIEDQTDIKVKLKPNFGKTT
                     FLYQALKSGDIDLYPEFTGTITSSLLKNPPKVSNNPKQVYNLAKNGILKQDKLSLLSP
                     MAYQNTYAVAVKKDYAEANQLKNISDLKKLDKLKAGFTLEFKDREDGSIGLQKHYGLN
                     LDISTLEPALRYQAINSKDVNIIDAYSTDSELIQYQLQILKDDKHLFPPYQGAPLLRQ
                     DTIKKYPQVKKALNKLAGHITEKEMQEMNYQVAVKHKSAATVAKQYLKAHHIIK"
     gene            complement(18067..20607)
                     /gene="mprF"
                     /locus_tag="VBC28_001058"
     CDS             complement(18067..20607)
                     /gene="mprF"
                     /locus_tag="VBC28_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HEO6815052.1"
                     /translation="MKKLIEKVKSLTSVIKIVFFISVLVLIIVEMIYLKRTISVEQLK
                     SVFGQLSPMNLFLIILVGVIAVLPTTGYDFVLNGLLRTDKSKRYILQTSWCINTFNNL
                     SGFGGLIDIGLRMAFYGKKGQEKSDLREVTRFLPYLISGLSFISVIALIMSHIFHAKA
                     SVDYYYLVLIGASMYFPVIYWISGHKGSHYFGDMPSSTRIKLGVVSFFEWGCAAAAFI
                     IIGYLMGIHLPVYKILPLFCIGCAVGIVSLIPGGLGSFELVLFTGFAAEGLPKETVVA
                     WLLLYRLAYYIIPFFAGIYFFIHYLGSQINQRYENVPKELVSTVLQTMVSHLMRILGA
                     FLIFSTAFFENITYIMWLQKLGLDPLQEQMLWQFPGLLLGVCFILLARTIDQKVKNAF
                     PIAIIWITLTLFYLNLGHISWRLSFWFILLLLGLLVIKPTLYKKQFIYSWEERIKDGI
                     IIVSLMGVLFYIAGLLFPIRAHITGGSIERLHYIIAWEPIALATLILTLVYLCLVKIL
                     QGKSCQIGDVFNVDRYKKLLQAYGGSSDSGLAFLNDKRLYWYQKNGEDCVAFQFVIVN
                     NKCLIMGEPAGDDTYIREAIESFIDDADKLDYDLVFYSIGQKLTLLLHEYGFDFMKVG
                     EDALVNLETFTLKGNKYKPFRNALNRVEKDGFYFEVVQSPHSQELLNSLEEISNTWLE
                     GRPEKGFSLGYFNKDYFQQAPIALVKNAEHEVVAFANIMPNYEKSIISIDLMRHDKQK
                     IPNGVMDFLFLSLFSYYQEKGYHYFDLGMAPLSGVGRVETSFAKERMAYLVYHFGSHF
                     YSFNGLHKYKKKFTPLWSERYISCSRSSWLICAICALLMEDSKIKIVK"
     gene            complement(20591..>21322)
                     /locus_tag="VBC28_001059"
     CDS             complement(20591..>21322)
                     /locus_tag="VBC28_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017770938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEO6815053.1"
                     /translation="WLHTKDGSDIYYRVVGQGQPIVFLHGNSLSSRYFDKQIAYFSKY
                     YQVIVMDSRGHGKSHAKLNTISFRQIAVDLKDILVHLEIDKVILVGYSDGANLALVFQ
                     TMFPGMVRGLLLNSGNLTIHGQRWWDILLVRIAYKFLHYLGKLFPYMRQKAQVISLML
                     EDLKISPADLQHVSTPVMVLVGNKDIIKLNHSKKLASYFPRGEFYSLVGFGHHIIKQD
                     SHVFNIIAKKFINDTLKGEIVEKAN"
BASE COUNT         7152 a         4083 c         3188 g         6899 t
ORIGIN      
        1 atttgaaagt gtatctaaaa taataaactc gccaattgag aattcatcaa aatagtctgt
       61 ccgtgtcctc cattcgggat gtttttcttc aatgtacttt ttatgaaatt ctaatccttc
      121 ctgctcatgt ctttgataat cagattgata ttcttctgtt gcaaccattt ttaaataata
      181 atcaaaaaac ttcttagctt gtctatttat catacctaca cctaattgat actttatatc
      241 actatcatat cattttttga atgaaactga atcaataacc acaactatta ctcattttat
      301 gattttagct aaatctatca tggcttgttc aatcttatgt tttccttttt gcatgacatg
      361 aagatagatt ttttctatct ctctactatc ttctgaatgt cctacaaact ctcgaataac
      421 gtttaggctt acattcttat cagccatgac tgaaacaaaa ctatgtcgga acatatgtgg
      481 aactatgtga acaggtctac caaaatcgac taggtcgtca atatccttat aggatttccc
      541 acttttctta ttatactttg cagactgact agttcctcct cttaaaaaca ttgaaatttc
      601 tgtatgatac aatggactcc cctgctgatt cttccccttt atgtatactc tagtaaagat
      661 atatcccata tcatgataag tcggattaaa tctagcttga aacttatttc tttcaatata
      721 tttcaataaa atttgttcta cttttggtga cattggaata gttctaacgc tttctttagt
      781 ttttagaatt gctctatatc gttccttctc atcacgcaag ccttcttctg ccatttcttt
      841 gtcagtctta ttatggcttg ccctctgcca atgaatctta atgagtttat tcttgaaatc
      901 taaaacatct tcattcaaac ctaatgcttc actcggtctc attcctgtca agtagataat
      961 tctgagtaca tcagcaacta attcattatt atgtcgctta gtccagtttt caataatctt
     1021 aaacacttgg ttagcctcgt caacttcaag atatttttca gctatatctt ccctaagttt
     1081 cgcctgctcc tctgcacttg tcagcgtctt acgtttaact ctcttattca gaatagggtg
     1141 ttgcaacgct gtcaattctc cctgttctac cgcccaatca aatatcatct taagatagat
     1201 atgaatattc tttgaagttc cctcacttgt attattaatg atagcttgca ttttttcctg
     1261 ttccatacag aactgtttaa ggctcatatt ttcgttgaaa tatggggaaa tatgctggtt
     1321 ataaacacta actctagtct ttagagtatt cttggcaggc ttaacacgat tgacccacca
     1381 cttaaaagct aaaccgtata attcgttgtt ttcatcatac ggtctagcta tctctttgag
     1441 gcataaagtt ttactaggct ctaatttctt tggtttttct ccagtcaaat ctaatacagt
     1501 ttcatcaatc atttcattta gtatctttga aacttttgta atcagctgac tattctttat
     1561 atcagaaata agtaacttct cgtgtccctt actgtccttt cctgttggta ttgccttagt
     1621 cgtataccac cctttatttt ctccccttgt aaggtactta taacgacttt ttgttatcaa
     1681 aggatgagta tatctcattg caaacttaac aagcacgttt ccttttttag aagggtctgg
     1741 tttccattga taaacagtca gaactcgagt agtcttacct accttcattt ttaccccctt
     1801 atactaagct ataactgcct cattttcatg gaaaatctta taatcaaacc aatctttaaa
     1861 cacctccgca tgaataaaga cattcccatg agttccacaa attccaaaag aatatggact
     1921 atttctcatt tgacgaatat agtgatccaa tgtaggtaat ttcataccct tccaaagttc
     1981 gtctaaaaat tctttcttcc gataccaatt tttagttaaa tcatagtctg actttgtctt
     2041 aagctctatc attggaatac tttcctttct tatgctataa tctagttagt taatttttag
     2101 aggcgactta ttctcaagtc gtcttttttg tccccaaaat tgagaactat tctgttctct
     2161 tatatattgc tctatatcct tttcatcatt atcagttagc cttgaaagtc cctctttgag
     2221 taagctttga atataatctt ccgccttttg aacatcatag gcctgatact ttgcaatata
     2281 gacttttgct aatgttttcg caacttgttt ctctgtccaa gcctcagact ggcgtagtgt
     2341 cgttttagcc cttgtaatac taagaactgt ctgattttcg gtctttagat aatctatcca
     2401 ccacttgacc ttttttcttc tccaacggtt gctatctgtc tcattaggac ggataaaacg
     2461 ataatgaccg ttgacagcct caaaatagat agatgataaa ggacgcttta gaaacaactg
     2521 ctccgcaata atattcgcct ttccctgcca acatctaagt tctgcacgaa tccaagacag
     2581 agattctaca acctcttgct tgccaagctg ctccatgcgt ttattataga tacaccattg
     2641 ttgattgcct cgtgaaccta tggcaatagt ttctccaact cgctcgccat tttctaaaat
     2701 agatttttca tgatattcaa agtgtttcgc tttagaaatg cacaatcctt ttttacagta
     2761 atcatatatc ctttgaacat ctaagctacc gtcaaatata tcgtgagcaa tatctaatcg
     2821 agtgaaattc acagtgtact gatataagcg acttattaaa gcaatccaat tattctcatt
     2881 accgtctaaa aattcttcat attggcgaca accttgcccc tttaattcaa taaatactcc
     2941 cattgaaatc ctatcatcat taaaatatac tttaatttct gaacaagaat agtgtcgctg
     3001 atatttattg attccccaat tattcaatgt gaaatcttcc aagggaatca gaagaatatc
     3061 agtcaaaact ctttctggga gaacattctt aatagttact gcaaaataat caattctgac
     3121 tgtgttcata ggattattct cctagaactt taatatcctc acacacaatc tttacacctg
     3181 cccagttact tcttactata ttccaagcca atctgacttt aggttgaatc aattcaacag
     3241 gaacgttcaa caaagactca ttttgagcaa gtttagaaat cttatctaaa tttccaataa
     3301 gttctaaggt aattgccttt aaatcatcag tagaaattcc catttcagtc aacactttta
     3361 ctttatccgc atcaagtact gtacaggtaa ttttagatat ttcttcggtc ttaactttct
     3421 caccattgat ttcattgtat ttataattta tctttgctga taaaagaaca acctttttag
     3481 gaatatcaaa ctgagcttta tctaaatcaa gcgtaccaat tttaatattt acatctgtca
     3541 tattgtaaac catatttttc tcctttaaaa ccgtatccta catgaaaata tatcttatcc
     3601 ttactctctc aatggtttgc gaccacttag tacaactaac aagccgtaac cccttacgta
     3661 ccaagggttt agagtacatt tcatgtatgt gtgcaccccc tattagaaat gggggtattt
     3721 tctgacctcc tgcaaagcag tcggtaggct ctttctacct agcgaaaaga ggaacgctcc
     3781 gctcgattct gcccccctgg gcaatcgtcc ctatttcgct agtccgaaag accgtcattg
     3841 tttctcatac tccaataaaa atcgcaagcc ttatcaaaat atttctcaaa atctagttca
     3901 tcagacttga atggaacttc aacatattta ggagtaggaa taactccgtc aatttgaata
     3961 tagcctgttc caatatcaca agtcttgtaa tcaatatctt gacttgaaaa catcatgcga
     4021 cacgaatctg aatcagcagt agtcgaacct aataagagtg ctgtgccaaa gttttgtctc
     4081 atatacgtcg gaatgaagtc acttgaggct ctctgcagac ctactataac ttcgatagag
     4141 gcttggcgag attttactaa tagacttcca aggttcgaca ttgcctcttg ataggcttgt
     4201 ttcccttctt tagtcgtttc tgcctctgtt ttgaaagcaa tcccttcatc aagcacgata
     4261 accagaggat agttcccctt gacatcagcc caagataaaa ctttcccttt agaaatatag
     4321 gtatctgggt tatctgccat aatctgaaaa cgtctattca tttcctcaac agcctctctt
     4381 aaagcaccag caatttgaaa aggagaatta taaactactt tatagcctaa cctagcctct
     4441 aactttttac caacaaccca agagagttct ccctttttgg ggtctagtaa cctaatttca
     4501 gaccctaaat gcaagtattg agccacaagg ttttcaatca tgactgtttt tcctgatttg
     4561 gttgagccag ccagtaatat atgaggagga gaaccgaatg tccaaccaac tttttctgag
     4621 atagttaact tcttagtttg aagagcatct tcccaagtca atctcttttc agcaaattta
     4681 ggaaaaatat acattacaga gttcgtgtat tcaatcattt ctcccaactc taaagataat
     4741 gcactagaaa gaagtgagct aagttgatta gctttagaat catacttact cccgtatttc
     4801 atacaagcaa ctcgaatatc ttctgtattc tcttgatact gaaggctgat atggttcata
     4861 atttcttctt tgccgttgac tactttactt tcaaagaagc cattagaaac gaccatataa
     4921 tttaaagcgt attcgatacc attaaaatac ttcttttgtc tcttcctcca aaatctaaag
     4981 acaactatag taattcctaa aaagatagta gcaacaaatg tattagctaa atttgtttga
     5041 aaattagcta taatactaat cgtaagtatt ccaaaaataa catagaaata aatagaaacc
     5101 tttttaccat acctaaaggg aagatataat atcggtttaa gatttcttac tctctgttta
     5161 ttccaaaaag aaaagcgatt aaagatattc atacaaacac ctcgcttact caagattttt
     5221 tgaacgcttt ttgtcaatga acattatgat gattgctgga agttggataa ataataccat
     5281 taaggaaagt gcaagggcgc atttgattga aagccaggga gtgaaggcag taaagtcaat
     5341 gccttttgcc gtaaatccag caaatactga tatgatagct aataatataa acgtgctact
     5401 cacccagaag taatattgct tattcattat atgtctcctt ttttattaat aaaggggagg
     5461 gaagtcctcc ccatgaaatt atttatagct actaaaatga tgaggtaact ttccatactt
     5521 tatgaaatca tctaacatag aagtaccgtt acagtatctc ttttggccct caaaatagaa
     5581 ggcgactaat ttttgtgtaa attcatcttg aatatttgtg attacaccta atgagtttag
     5641 caaataggcg taggcttcac tataaacatc agatttagtc ttatctgata agttaaggcg
     5701 tgctttattt gcaacaaatt tgcgttttgc tggggtatta aagacttgat tcatgctctc
     5761 gacagaactc cgaacatgac taggatagct tgtataactt gcttttgaca tagtattctc
     5821 cttttaatta aatttagatt ggttttcaat tatatcttac atggtgatgc tcttgcccgc
     5881 attctcccct ccttttaagt ggttttttta tcccgtaatt ggttaaattt aaacagagtt
     5941 ctacactccg tttaataatc attattctac ttattcgtaa gtaataagta atgaaatttt
     6001 aagcataatc taacacaaaa agctcgatga attcgttttg cttgaaagac ctccaaaaag
     6061 taattttgta attcagaaat caaatcaagg acatcttttt ctaacataaa ggaggtttga
     6121 taatcccagt cattactctc tttaggagat gacagagcca aattcctctt ttttagagac
     6181 tgccagtcaa tttcatgcat ttttggaaca gttttcctaa ttgcctgact aacgatactg
     6241 gagtagctag cttcaagacc aatttcttca aaaatttgtc gttgtaattc atctaatatt
     6301 aattttgatt cagttgctaa acgcatacgg atattttctt tttccataat ttcacctctc
     6361 aattaaagtg gtttttttat cccgtaattt aatgataaca aaatttatag gagctgtcaa
     6421 tacttttata aaaataactg atttttaact gattaaactc ccacaaagtc acattcttct
     6481 atttatcatt tattttctcc aatataaaaa gtctatcaca ttaaggtttc taagtattat
     6541 tatatttcct tataactaca tcttctttac agaagttaaa taattgatta taaaaaagcc
     6601 tatgaaatca atatttctgg gctttttatt tttaaaaata aaatctgaca aaaaagtata
     6661 aatttacctc ttaccttcag gtagttagtc attcttttaa aactcacttt tttataactg
     6721 aaaatattgt tcttaatttt aaaaattcat tttagattca ttttacgact atatactcag
     6781 aagtaacaaa cctaatccaa ggtttgaaaa aagaaagaag gaagtcagta tgacaaacta
     6841 taaaaacaac tttaaaaaat tcatgagcaa taaattatac gtagggttag cttccattac
     6901 gctacttggt attgggggag ctataggagt aagtggagct gcattagcgc ataaccatca
     6961 ctcagaacac atgtcttttg aacataagca tgaaggacac gataaacatc acgatcatag
     7021 agaaaaatac tcactaaaaa cattaaagaa tatctcgaca aaaatcgatt ataagactgc
     7081 cttagaaact gccaaaaaag aagctcgctc aggctttatt tcacaaattg aattaaatac
     7141 caaaggtgat acaccttttt atgaaatcaa agttattgat aataaaatag ctaaaaaata
     7201 taaaattgat gctaattctg ggaagatcct ttcaacaaca tcagataatc aactcaattc
     7261 aaaagatgcc caattaaaac aaccaagtaa agatattcag actgcattac aagctattca
     7321 agcaaaatat aaaaatgcaa aaataaaaga aatttctctt gcgaataaca aatcaaataa
     7381 gctagtttat aaagtaaaac tacttgatgg aaatactcct aagattgccc aatttgatag
     7441 tgataaagca tcatttataa aggcaaataa gcttaataca actcataaaa atcacgacta
     7501 atactagtgt attaaaggga ataggacctc agtactccat tcccttttgt gatataatgg
     7561 aaaaaagcca tacggaggcc aaaaaatgaa aatccttgtt gttgaagatg agtttgactt
     7621 gaaccgtagt attgtaaaac ttttaaaaaa gcaacactac agtgttgaca gtgcttcaaa
     7681 tggtgaagaa gccttacaat ttgtttcagt ggcagaatat gatgtgatta tcttagatgt
     7741 aatgatgcca aaaatggacg gcttcacctt tttaaaactg cttcgcaaca aaggaagtca
     7801 agtatctatt cttatgttga ctgctcgaga tgccgttgaa gatcgtatcg caggtctaga
     7861 ttttggtgcg gatgattacc ttgtaaaacc atttgaattt ggagaactca tggcccgaat
     7921 tcgagctatg ttacgacgtg caaatagaca agtatcttct gatgatattc aaattcaaga
     7981 tataacaatc aatttatcta caaagcaagt ttggagaaac gacaatttga ttgatttaac
     8041 agctaaggaa tacgaagtcc ttgagtattt ggcacgtcac agagaccaag tcctctctcg
     8101 tcatcaaatt cgtgaacacg tttgggatta tgattattat ggagagtcca atattattga
     8161 tgtccttatc aaaaaccttc gtcgaaaact agataacaat cgagatggat cactaataaa
     8221 aactaaacga ggtttaggat atgttattcc aaagtaaaat aaattaccct aaaaagtcta
     8281 gcatcatcac tcaaattacc ctctggtaca gctcttttat ttttatcctt gttatcggtg
     8341 tattaattgg ttcatttttt atttcaaaaa gcattgctga aaacaaatct aaaaaaaatt
     8401 tagaagctaa agctgtgcaa atgagtcaag cgttagctaa aggtcatcgg tatgaggctt
     8461 ttgaagatgg aatattttat tcagtctatg accaaaatgg aaaagttatc tattctggtt
     8521 ttccaaaagg atttaaaagg gatttagacc atcaacataa acataaaaag aaattatctc
     8581 tattttccat ggagaatcgt acctttcagt atgttgatat tccaatttct gggaaaaatc
     8641 agtggttacg tgctatccgt actgttgaca gactggataa gcaattaact gaattgttat
     8701 ttagtttggg tatcgttctt cctcttatgt tgattataat aactgttggt ggctatttaa
     8761 ttttaaagcg cacttttaga ccgattcaag aaattactga aaccgctcaa tttattaccc
     8821 aaaatgaaga ctatactaag cgaattatca ctaaaaataa cgaaaatgaa ttaactgagc
     8881 tagcagctgt tatcaatact atgttggctt ctattgaatc atcatttgtt cgggaaaaac
     8941 aatttaacaa tgatgtttct catgaactac ggactcctgt aacagttata ctttctgaaa
     9001 gtgaatacgg aaaaaactac gcagaaaatt taagtgaagc aaaagaatcc tttgaggtta
     9061 tacaccgtca gtcactatca atgaaaaaat tagttgagca acttctagaa ttgactaagg
     9121 cagaaaatcc attatcaatt cagttagaac ctcttaattt ctcaattatg atgaagcaac
     9181 tagtatccga ttcgagtaga ttattagata atacccctat acaccttgac tctcaaatcg
     9241 aagatgacct ttggattata ggtcaacaaa cactactcaa acggcttttt gataatctat
     9301 tttcaaatgc tattaaattt acaaacaatc atatttctat tagtctaaga cagtccgata
     9361 atcaaattgt cttctctatt aaagataatg gtctaggtat ctcagttgat gaccaaagta
     9421 aaatttggaa ccgtttctat caagtggact ctgctcgaac caaggatagt cagtctggaa
     9481 ttggtttagg gctatcttta gtaaaacaaa tcgctacaat acaccgtgct aaaatatggg
     9541 ttgattctaa acctgatgat ggcagtcagt ttacgctcac ctttccaaaa ttgaagaaag
     9601 actagagaaa ggaaataaaa aaaacgatag attacaccta tcgttttttt tatttaactt
     9661 aaactcaatt tattatatct cttcagatat ttttgagact aatagctgaa atctttaaag
     9721 tattaaaact tgtagaacta acaaaccgcc accaaatatt aggaggaata aaacaaccgg
     9781 ccacacaaat tttagccaat gggagtactt catatctaac atctgcaagg ttgccataac
     9841 tagtccagta ggtgctaaaa acaacattgc atactgccca aattgataag ccattactac
     9901 tactgaacga ggaatcccaa cagtatctgc aagaggtgct agaattggca ttgaaagtac
     9961 tgctaaaccc gacgaggatg gaacaacaaa tcctaacaca aagtagataa acatcataac
    10021 aatgataaag acagagccac taacatgtga agctaatttt gaagctgtgt aaagcatcgt
    10081 atctgatata taaccttggc tcaaaataat attgatacca cgcgctaaac caatgattaa
    10141 tgaaacccca acaaggcttg atgcaccatt aacaaactca tcaacaacat ccctttctcc
    10201 aataccattt gccccagttg ctgttaaaaa cataataata attgtaatag ctaaaaagga
    10261 agaagccatg gttggaaacc accatccctg tgacattact ccccaaacca tcaaaggaaa
    10321 tgaaataaca aataaactta aaataatttt ttgtctaata gtaaagagtg agggtgtatg
    10381 gttagttgtc atgccccatt tagcattaaa ttctactcta tcttcatagg aatatgaaaa
    10441 ctctggattc gcttttattt ttttagcata ccaatgtaga tacaccacaa caaaaatagc
    10501 acctgcaata caccctgctg tacgccatga taagccttct gtgaaactaa taccagctgc
    10561 atttgaagca ataacagaag aaaacggatt aattgttgaa aatgatgtcc caacagaact
    10621 tgctaagaaa atagcaccta cacaaatgat agaatcataa cccatggcta aaaagattgg
    10681 aactaaaata ggataaaaag caactgcttc ttcttcaata ccacatagag taccgcctaa
    10741 aaccatcaat aaggaaacta aaaatatcaa taaaaactct ctacctttgg ttttctttgt
    10801 taaagctaga agaccagact caaaagcgcc actctttcta actactccta tcatgcctcc
    10861 aagtaccatg ataaatacca taatatcaac agcttcaatg gttccattta ccatactagt
    10921 cgtcacggag ccaatgcctg ctggattttg cttcaaacgt ttgtaagtat ttggaactga
    10981 aactggctta ctaatagcac cagaagtaaa ttctttaatt ttaattttga cattcatctt
    11041 atccaactgt gtttgcgttg ctggaacatg aactgtctta ccatttggat ctgttactac
    11101 tagtttggat gatgctgtat caaattgtaa ttttgaatag gacccagcag gtatccacca
    11161 tgtagccaaa accgaaaaga ttgttaaaat gaataatatt gtaaaggcac ctggcattcg
    11221 aaaaccttta ctccttttgt tttcactgtt tgtcattttt tctggcatga cacgcctcct
    11281 ctataataaa aatagattga cttcatcaga aaaagtgcaa agatgaaggt taaataatca
    11341 tttctttgca cttatctgag attacttagt tatacgtgtt cctaatttcc catctaatgc
    11401 atcttctaaa ccatttaatg aggtgattaa ggctgttcct ttcggattgt gatctaagaa
    11461 actcatacag gctattactt taggtaacat acttcctttt gcaaactcac cttggttaac
    11521 gtaatcaatc atttgattct cattaacctc tgttaaagct ttttgatttt ctttaccaaa
    11581 ttgagtataa acataatcaa ctgcagtgag aataatcaat tgatccgcag ctagttctcc
    11641 tgctaacagt gcactagatt tgtctttatc aataacagct gcaatacctt cataacctcc
    11701 cttattaatg acagggatgc ctccacctcc acctgcgata actagggtat cattttcaat
    11761 aagtgtttta atgctattaa tttcgatgat ttttttaggt tcaggtgaag caacaacacg
    11821 acgataccct cgtccagcgt cttctacgaa agtataccct ttctctatag caatcttttc
    11881 tgatgtctcc ttatcataaa aggtgcctat aggcttggta ggtgcgctaa atgcttggtc
    11941 tgattcatcc acctcaattt gggtaataat tgtagcaact tccttattta ttccctcttg
    12001 acgtaattca tttaaaaggc tctgttggag atgatagcct atgtatcctt ggctcattgc
    12061 tccacattct ggaaaaggaa aattagttcc ttgaccactc tcagccgcaa aattcattcc
    12121 taaattaatg gcaccaactt gtggtccatt accatgactt acaacaattt cgtgtccctt
    12181 ttttatcaag gccaccaatg attttgcagt atgttttact aatcttaatt gttcttctgg
    12241 agaatttcct aaggcatttc ctcctagtgc aacaacaatt tttgccatga aaattcctcc
    12301 taatcaccta aagttgctac cataactgct ttaatagtat gcatacgatt ttcagcttct
    12361 tggaaaacaa ctgagtgagg gccttcaaaa acttcatctg atacttccat ttccttcaaa
    12421 ccatattttt cataaatgtc gtatccaact ttagtatcta tattatgaaa tgagggcaag
    12481 caatgctcga aaataacgtt tggattttct gtcatattaa gcatttcttg ggtaatacga
    12541 tagggttcta aaagagctat tctttctttc caaacttcat ccggttctcc cattgaaacc
    12601 caaacatctg tatataggac gtctgaatct ctgacgcctt ctgcaatatc agcagtgatt
    12661 tcaatagaag caccagtagt tttcgcaatt tcttgacatt tagagagtag ttcaggatct
    12721 ggctctaatt ctttaggaca aaccaaatga taagtcatgc ctacaataga agcaccgatc
    12781 atcaaggcat tagcgacatt gttacgtcca tcacccacat acgtaaagcg aatatcttta
    12841 tagggcttat gtaagcattc cttagcggtc agaaaatcgg ctagtacttg cgttggatga
    12901 tcagcatcgg ttaaaccatt ccacacagga actcctgaaa attcggctaa agtctcaact
    12961 gtttcttgag aaaatccacg atactcaata ccatcataca taccacctag cacacgcgca
    13021 gtgtcttttg aggtctcttt tttacccatc tgactgcctg ttggacctaa ataagtaaca
    13081 tgagctcctt gatcgtgagc agcaacttca aaagcgcaac gtgtccgtgt agaatctttt
    13141 tcaaaaatca aagcgatatt tttaccaaca agtttttgtt gttcaattcc tgcgtattta
    13201 gcacgtttta aatcttctga aagttttaaa agaaattcaa cttctgctgt actaaaatcc
    13261 aataaagtaa gaaaactacg gttacgtaaa tttttcattg cttattgcct cctttttttt
    13321 tatactctta ttgtagatat cgctttcaaa aataatcaaa attcatcaaa aatcttcaaa
    13381 aaaactttaa ggacatttat atgctagata tattatcaga aaaacatcta gaaagaaggc
    13441 agcgtattat tatctctgca atagctatcg ccttagctgc tcaaatcaat atctcaatac
    13501 ttgctgatgg gtttattatg accttatctt tgttcatttt gcctgtcttt ttatatttta
    13561 atgacgacat taatccaatt cttctctgtc taggaattac ttttgcttct cctatcttca
    13621 gaggaatcat attaagtatc gctggcgagg ctgagattca tcaaataatt gaattcgttt
    13681 taactgatat ggcgttttat atttgttatg gcatcacctt ttatactatt tattggcatc
    13741 gttcctaccg taataaaggt acttttttct tttctatcat tatttgtgat tattttgcca
    13801 accttgtaga aattagtttt ctcataaagt ttaacaatta tactataact atattcgcta
    13861 ctttatttgc catagcactt ttgcgagctt ttattagttg cgctgtggct tatacttata
    13921 gttatctatc acttctctta caaaaagacg atcacgaacg tcgatactat tattttatgt
    13981 ggtcaacttc agccgttaaa agcgaggttt attttatgca aaaaaatatc attgaaattg
    14041 aaaacatcat gaaaaatgct tatttattag ataaagagtt atccaaatat catctaccta
    14101 aagaatacca acacctctct ctagatattt cgcgggacgt tcacgaagtt aagaaagatt
    14161 accaaaatat tattaaaggc ttaggaacct attttagtgt aaaaaatgaa tcaactatgg
    14221 cattgaaaga tatctttcaa attgttttat cttatactag gagtattatt caatttcggc
    14281 atcaagatat cattattctc gaaaataata aatgtaattt gataatatca aactattact
    14341 atttactaac cattatttcc aatatcgttt taaatgcagt agaagctatt gataaacaaa
    14401 aaaagggtac aatcagtgtt cacacagaag aattagaaga ttttattaaa attgagatta
    14461 gtgataacgg accaggaata cctgataaaa tgaaacacat gattttcaaa cctggatttt
    14521 cgactaaatt tgatgctaat ggtgatatct atcgaggaat cgggctatcc cacgtgcgta
    14581 ttttaatgga agagcaatat caaggaacga taactgtttg tcctaatcag ccaaatggaa
    14641 caacatttac actgttattc cgtaaaaatc gatttaagtt aggagataat acgtgaaatt
    14701 ctatattatt gatgatgacc ccactatcac tatgatttta caagatatta ttgaagaaga
    14761 tttcaataat acagtagtca gagtgaataa tgtctccagt aaagcataca acgaattgct
    14821 gattgcagat gttgatatcg tattgattga tttattaatg ccaatattag acggtgtaac
    14881 attagtccaa aaaatctata aacagcgttc cgatttaaaa tttattatga tttcccaagt
    14941 caaagataat gatttacgac aagaagcata taaagcagga atcgaatttt ttattaataa
    15001 acccattaat attattgaag taaaaagcgt cgtgaaacgt gtcactgata ctatcgaaat
    15061 gcaaaaaaaa ttaaatacaa tccaaaatct cctagaaaat acgccatcat atcaaaagcc
    15121 tataacgact agtaacctca ctaaaatccg ttcaatactc agttatttag gtattacttc
    15181 agagacagcc tacacagata ttcttaacat ctgtgaactt ttattaaaac aagaattaaa
    15241 ttttgcccaa tttgattttc aaaaagaact ttctatcgat gaacaccaac aaaaaatcat
    15301 tttacaacgt attcgtcgtg ccgttaaaaa agctatgata aatatggctc atctgtacat
    15361 tgatgatttt gaaaatgaat taacactgca atatgctaat gcattgtttg gatttcagaa
    15421 tatccataat gaggcacaac ttattcaagg aaaaagcatg tatggtggta aaatttcact
    15481 taaacatttt ttcgatgaac ttattttaca gagtaaaacg ttttaagact tgtttaagca
    15541 tggccttata tactataacc atcctaaaac ttttcttttc ataaatctcc aacaaacacc
    15601 ttgtctaccg caaggtgttt gtttttgctt ctagtttatc gtttctgaca atttaaatgt
    15661 cagatagttg tttgaatgtc tactttatgc taatataacc ctaatatcat acgacctaca
    15721 ctcgttacgc actagaaagt aaattacatg atttcatttg aaaatgttag taaatcatat
    15781 ggtgatcata ccatcattga taatattagt tgccatattc aaagaggaga attctttgtc
    15841 cttgtggggg ccagtggcag tggtaaaact accatcctca agatgattaa tcgtctcatc
    15901 gaaccaagcc aaggcgctat tactttagat ggggaaaaca ttacatcact tgatttgcga
    15961 caattacgct tagaaactgg ttatgtcttg caacaaattg cacttttccc taatttaaca
    16021 gttggagaaa atatagaact tatccctgaa atgaagggct ggtcaaaagg tgaccaaaaa
    16081 aaaagccgct agcgaccttc ttgataaggt gggtttacct gctaaggact actttaatcg
    16141 ctatcctcat gagctctctg gtggggaaca acaacgcata ggtatcctac gagcaatcgt
    16201 tgctaaacct aaggtactac ttatggatga gccattttct gctttagacc ctatctcaag
    16261 acgtcaatta caagatatca ccaaacaatt acagtctgaa ttgggtatta cattagtgtt
    16321 tgtcacccat gacatgaagg aagctatgcg tcttgcagat agaatttgtg ttattaagga
    16381 aggtaagata gtgcaattgg atagaccaga aataattcaa aataatccct ctgaccaatt
    16441 tgttagaacc ctatttgaag aggagaactg agtatgacaa cactaatcac tacctttcaa
    16501 gagcgttttg gagattggac tcaaagtctt attgaacacc tccagctatc actactaaca
    16561 cttattttag caactttaat tgctattcca ctcggtatca tcattagtca ttataaaaaa
    16621 atcagtcatg ttgtccttca gataactggg atatttcaaa caattccttc tttagccttg
    16681 cttggtttat ttataccttt tatgggaatt ggaactgtac cagctgtcgt tgcactaatt
    16741 atttacgctc tatttccaat tttacaaaat actgtaacag tactgatgca aattgatgct
    16801 aatctcattg aggcagcaac cgcttttgga atgacacgtt gggaaaggct taaaaagttt
    16861 gagttagctt taagtatgcc tgttatcatc tcaggcatta gaactgcctc tgttatgatt
    16921 attggtacag ctacattagc tagtcttatc ggagctggtg gccttggatc atttattttg
    16981 ttagggattg atcgcaacaa tccttctcta attctcatag gggcaatctc ttctgctgtc
    17041 ttagctatta tatttagtgg tttaattgga ttactagaaa aggctagatt acgaaccata
    17101 gccgtttctg gtatactttt actagcaggc ttaggactat cctatgctcc aaaatggatg
    17161 cctggaacaa atactgcaac gataactgtt gcagggaagt taggaactga accagatatt
    17221 ttaattaata tgtacaaaga acttattgag gatcagacag atatcaaagt taagcttaaa
    17281 cctaatttcg gaaaaacaac tttcttatat caagccctaa aatctggtga tattgatctt
    17341 tacccagagt ttacaggtac tattacatca agtttactta aaaatcctcc taaagtaagt
    17401 aataacccta aacaggttta caacttagcc aaaaacggta ttttaaaaca agataagttg
    17461 tcacttttgt cacctatggc ttatcaaaac acctacgctg tagctgttaa aaaagattac
    17521 gctgaagcta atcaactcaa aaatatcagc gacctaaaaa aattagataa actaaaagct
    17581 ggtttcactt tagaatttaa ggatagagag gatgggagta ttggtttaca gaaacattat
    17641 ggtttaaacc ttgatatctc aaccttagaa cctgctttac gataccaagc tatcaatagt
    17701 aaagatgtca atattattga cgcttactct accgatagtg aattaatcca gtatcaactt
    17761 caaattctca aagacgataa gcatctattt ccgccatatc aaggagcacc gctactaagg
    17821 caagatacga ttaaaaaata ccctcaagta aaaaaagctc tcaataaact tgctggtcat
    17881 atcactgaaa aagaaatgca agaaatgaac taccaagtag ctgtaaagca taaatcagca
    17941 gctacagtag ctaaacaata tttaaaagca catcatatta taaagtagat tcaagcaatc
    18001 aatggtatta aatgatttaa aaagagcatg tcgcatgaca tgctcttttt aattgccaaa
    18061 taaagcttat ttaacaatct taattttact atcttccatt aatagggcac aaatagcaca
    18121 aattaaccag gacgaacgag aacaagaaat ataacgttcc gaccacaatg gtgtaaactt
    18181 cttcttatac ttgtgtaaac cattaaatga gtagaaatga ctaccgaaat gatagacaag
    18241 atacgccatt ctctctttag caaaacttgt ttcaacgcga ccaactcctg ataaaggtgc
    18301 catccccaaa tcaaaatagt ggtatccctt ctcttgataa taagagaata atgataaaaa
    18361 gaggaaatcc ataacgccat tcggaatttt ctgtttatcg tgacgcatta aatcaataga
    18421 gataatactc ttttcatagt ttggcataat attagcaaaa gcaacaactt cgtgttcagc
    18481 attttttacc aaagctattg gggcttgttg gaaataatct ttattaaaat atcctagtga
    18541 gaaacctttt tcaggacgtc cttctaacca agtattagaa atctcttcca aactatttag
    18601 tagctcttga ctatgtggcg attgtacaac ttcgaaatag aaaccatcct tttcaactct
    18661 atttagggca tttctgaaag gtttgtactt attcccttta agagtaaacg tttctaaatt
    18721 aactaaagca tcctcaccaa ctttcataaa gtcaaaacca tactcatgta aaagtagtgt
    18781 caacttctgt ccaatactgt aaaaaacaag gtcatagtct agcttatcag catcatcaat
    18841 aaacgattca atagcttcac gaatataagt gtcatcaccg gctggttccc ccataataag
    18901 acatttatta ttgacaatta caaattggaa cgcaacgcaa tcttctccat ttttttggta
    18961 ccagtagagc cttttatcat ttaaaaaggc taaaccgcta tccgaagaac caccgtaagc
    19021 ttgaagtagt tttttataac gatccacatt gaacacatca ccaatctgac aagattttcc
    19081 ttgtaaaatc ttaaccaaac ataaataaac gagagtaaga atcaacgtag ccaatgctat
    19141 cggctcccat gctatgatat aatgcaggcg ttcaatacta ccacctgtaa tatgagccct
    19201 gatagggaat agtagtcctg caatataaaa tagaactccc attaaactaa cgataatgat
    19261 tccatcctta atacgctctt cccagctata aataaattgt tttttataga gagttggctt
    19321 aatgactaat aagcctaaca atagtaaaat aaaccagaaa gatagtcgcc aactaatatg
    19381 acctaaatta agataaaaca atgtcaaagt aatccagata atagcaattg gaaaagcatt
    19441 tttcactttt tgatcaatag ttctagctaa gagaataaaa caaaccccca gcaataaacc
    19501 tggaaactgc cataacattt gttcttgtaa tgggtccaag cctagcttct gcaaccacat
    19561 aatataagta atattttcaa aaaatgctgt tgaaaatatt aagaatgcac ctaaaatacg
    19621 catcaaatgg ctcaccatgg tttgtagaac agttgatact aactctttcg ggacattttc
    19681 ataacgttga tttatttgac tacctaaata atggataaag aaatagatac ctgcaaagaa
    19741 tggaataata tagtaggcta aacgataaag taataaccat gcaaccacag tttctttagg
    19801 tagtccctcg gcagcaaacc ctgtaaatag aactaattca aaacttccta atccaccggg
    19861 aataagggat acaatcccga cggcacaacc aatacaaaat agtggtaaaa ttttataaac
    19921 tggtagatga atgcccatta aataaccgat aattataaat gctgcggccg cacatcccca
    19981 ttcaaaaaaa gaaacaacac ctaattttat acgagtacta gatggcatat ctccgaaata
    20041 atggcttcct ttatgaccag aaatccaata aataacagga aaatacatac tagcaccaat
    20101 taataccaaa taatagtaat caacactagc tttggcatga aaaatatggc tcatgattaa
    20161 ggcaatcaca ctaataaatg acagaccaga aataagatag ggtaaaaaac gagtcacttc
    20221 tcttaggtca ctcttctctt gacctttttt accataaaaa gccatgcgca acccaatatc
    20281 gattaagcca ccgaatcctg acaagttatt aaaagtgttg atacaccaac tagtctgtaa
    20341 aatatacctt ttgcttttat ctgtacgtaa aagtccattc agtacaaagt catatccggt
    20401 tgtcggtaag acagcgataa cccccacaag gataattaag aaaagattca ttggagataa
    20461 ttgcccaaaa acactcttta gttgctcaac agaaatagtt cgtttcaaat aaatcatttc
    20521 aacaataata agcactaaaa cagatataaa aaatacaatt ttaatcacag aagtcagtga
    20581 tttgactttt tcaattagct ttttcaacaa tttctccttt caacgtatcg ttgataaact
    20641 tttttgcaat aatattaaaa acatgggaat cttgcttaat aatgtgatgc ccaaagccaa
    20701 ctaaagaata aaactccccc cttggaaaat aagaagcaag tttcttagaa tgatttaact
    20761 taattatgtc cttatttcca accaaaacca ttacaggagt tgacacatgc tgtaaatcag
    20821 ctggactaat cttcaaatcc tccaacataa gcgaaataac ttgagctttt tgcctcatat
    20881 acggaaagag tttccctaaa tagtgaagga atttataggc aatccttact aaaagaatat
    20941 cccaccatcg ctgaccatga atagtcaggt tccctgaatt aagcaaaagc cctctaacca
    21001 tacctggaaa catcgtttga aaaactaaag ctaaattggc accatcgcta tagcctacca
    21061 atataacttt atcaatctct aaatgaacta agatatcctt taagtcaact gctatttgcc
    21121 tgaaactaat ggtatttagc tttgcatgac ttttgccatg ccctctacta tccataacaa
    21181 taacttggta atacttagaa aaatatgcta tttgcttatc aaaatagcga ctacttaagc
    21241 tattgccatg taaaaaaaca atcggttgac cttgaccaac gacacgataa taaatatctg
    21301 aaccatcttt agtatgtaac ca
//
LOCUS       DATRMA010000030        21235 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.030, whole genome shotgun sequence.
ACCESSION   DATRMA010000030 DATRMA010000000
VERSION     DATRMA010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21235
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.030"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            16..1137
                     /locus_tag="VBC28_001060"
     CDS             16..1137
                     /locus_tag="VBC28_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018366757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEO6815054.1"
                     /translation="MATYRQRGKKKLWDYRIFNEKSELVASGSGFKTKREAMNEAMRI
                     EQQKLLVNAISSDITLYDLWFEWYSLIIKPSNLAETTKNKYFTRGSVIRKLFGNQKVN
                     KIKHSAYQRKLNTYAEKYTKNHVRRLNSDIKKAIQFAKRDGILLSDFTDGVVIAGRQF
                     VKDADDKYLHSISDYKKVISYLENNLNYSNSIVYYLLLVLFKTGLRVGEALALTWDDV
                     NFEDLEIKTYRRFSGDKGTFSPPKTKTSIRTIPISQSLALILRDLKDDQQVMLKNLKI
                     VNINNQIFYDYRYGVSSNSAINKSLRNVLHLLNIDSKMTATGARHTYGSYLLAKGVDI
                     WVVARLMGHKDITQLLETYGHVLTEVINKEYETVRSLVS"
     gene            complement(1238..1310)
                     /locus_tag="VBC28_001061"
     tRNA            complement(1238..1310)
                     /locus_tag="VBC28_001061"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1275..1277),aa:Lys,seq:ctt)
     gene            complement(1592..2860)
                     /locus_tag="VBC28_001062"
     CDS             complement(1592..2860)
                     /locus_tag="VBC28_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEO6815055.1"
                     /translation="MADNLALRMRPRNINEVIGQQHLVGNGKIIDRMVAANMLSSMIL
                     YGPPGIGKTSIASAIAGTTKYAFRTFNATVDSKKRLQEIAEEAKFSGGLVLLLDEIHR
                     LDKTKQDFLLPLLENGNIIMIGATTENPFFSVTPAIRSRVQIFELEPLSNEDIKKAIQ
                     LAISDKERGFPFLVTIDDEALDFIVTATNGDLRSAYNSLDLAVMSTSPNEDGSRHISL
                     ETMENSLQCSYITMDKNGDGHYDILSALQKSIRGSDVNASLHYAARLVEAGDLPSLAR
                     RLTIIAYEDIGLANPEAQIHTVTALEAAQRIGFPEARILIANIVVDLALSPKSNSAYL
                     AMDAALADLRRSGNLPIPRHLRDGHYSGSKTLGNARDYKYPHAYPEKWVKQQYLPDKL
                     VGHNYFEANETGKYERALGSNKERIDKLSD"
     gene            complement(2853..3344)
                     /locus_tag="VBC28_001063"
     CDS             complement(2853..3344)
                     /locus_tag="VBC28_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001879696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO6815056.1"
                     /translation="MIIRPMQDCDREAVLTLQNTGWTALTSPVYDRKWTESDLEKNLA
                     NGMSFFVAEVDDKIAGVLDFGPYYPFPAGKHVATFGILIAEPYQGQGLGKALLKALLT
                     EAKAQGYIKIAMHVMGNNSRAISLYQKYGFTEEARITKAFFIENHYVDALIFAKDLEN
                     NHG"
     gene            3671..4141
                     /locus_tag="VBC28_001064"
     CDS             3671..4141
                     /locus_tag="VBC28_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HEO6815057.1"
                     /translation="MAKFGFLSVLEEELDKHLQYDFAMDWDKKNHTVEVTFILEAQNS
                     SAIETVDDQGETSSEDIVFEDYVLFYNPVKSRFDAEDYLVTIPYEPKKGLSREFLAYF
                     AETLNEVATEGLSDLMDFLTDDSIEEFGLSWDTDAFENGRAELKETEFYPYPRY"
     gene            4277..4735
                     /locus_tag="VBC28_001065"
     CDS             4277..4735
                     /locus_tag="VBC28_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000350740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEO6815058.1"
                     /translation="MDIFDELFDFSGAKIALFCEGKILTSLRDDFPDLPYAGFWDLPG
                     GGREDNETPLECLFREVDEELSLTLTRNHIDWVKTYRGMLKPDKLSVFMVGHISQKEY
                     DSIVLGDEGQDYKLMSIDEFLSHKKVIPQLQERLRDYLEVEDDYFTKGGS"
     gene            4707..5177
                     /locus_tag="VBC28_001066"
     CDS             4707..5177
                     /locus_tag="VBC28_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO6815059.1"
                     /translation="MITLQKAEASDLEKIIAIQRASFKAVYEKYHDQYDPYVEEVEQI
                     RWKLVERPDCFYHFVLVDETIVGFLRLVIKDEEKRAWLGTAAILPQYQGQGYGSAAMA
                     LLEKTYPKLTKWDLCTIAQEKLMVSFYEKCGYHSTHTEPEQEGMDMVYMTKKIE"
     gene            complement(5216..5932)
                     /locus_tag="VBC28_001067"
     CDS             complement(5216..5932)
                     /locus_tag="VBC28_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEO6815060.1"
                     /translation="MKTVKEVSILSGVSVRTLHHYDKIGLFPPTALSEAGYRLYDDEA
                     LIRLQEILLFRELEFPLKDIKYLLEQAKEERQDLLAQQIKLLEWKRSHLEQVITHAKR
                     LQEKGDDYMNFDVYNKTELEQLQAEAKEKWGQTAAYKEFAQKHASDDFAQISQEMAKI
                     MVQFGQLKTQNVSDESVQMCVKRLQDYISQHFYTCTNEILAGLGQMYQSDDRFSQSID
                     KAGGAGTSEFVSQAIAYYCQ"
     gene            6056..6514
                     /locus_tag="VBC28_001068"
     CDS             6056..6514
                     /locus_tag="VBC28_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011074755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MepB family protein"
                     /protein_id="HEO6815061.1"
                     /translation="MEIVELLREWYSSFEVKEEKWNEKYEGILIRIEGDGNYKRCRLA
                     HKTASKMGYFTVFWQKDGNGKNIPFYEQDEVEDLIIVIKDGRWKGLFIIPKEVAVSKG
                     ILSSANSQGKMAMRFYPPWCSDLNRTALVTQRWQLNYFIDLSRNNEGVTT"
     gene            6511..7464
                     /locus_tag="VBC28_001069"
     CDS             6511..7464
                     /locus_tag="VBC28_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEO6815062.1"
                     /translation="MNTWNELTVHVNREAEEAVSNLLIETGSQGVAISDSADYLGQED
                     RFGELYPEVEQSDMIAITAYYPDTLDIEAVKADLADRLANFEGFGLATGSVNLDSQEL
                     VEEDWADNWKKYYEPARITHDLTIVPSWTDYEAKAGEKIIKMDPGMAFGTGTHPTTKM
                     SLFALEQVLRGGETVIDVGTGSGVLSIASSLLGAKDIYAYDLDDVAVRVAQENIDMNP
                     GTENIHVAAGDLLKGVQQEVDVIVANILADILIHLTDDAYRLVKDEGYLIMSGIISEK
                     WDMVRESAEKAGFFLETHMVQGEWNACVFKKTDDISGVIGG"
     gene            7464..8204
                     /locus_tag="VBC28_001070"
     CDS             7464..8204
                     /locus_tag="VBC28_001070"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001188249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEO6815063.1"
                     /translation="MQQYFVNGEAGAYVTIEDKDTIKHMFNVMRLTEDDQVVLVFDDA
                     IKRLAKVVDSSAHRFQILEELDNNVEMPVQVTIASGFPKGDKLDFVTQKATELGAAAI
                     WGFPADWSVVKWDGKKLAKKEDKLAKIALGAAEQSKRNRLPQVRLFEKKADFQAELAG
                     FDKIFIAYEESAKEGELSALAQNLQTVKAGDKLLFIFGPEGGISPKEIAAFEEVGAIK
                     VGLGPRIMRTETAPLYALSVISYSAELL"
     gene            8201..8788
                     /locus_tag="VBC28_001071"
     CDS             8201..8788
                     /locus_tag="VBC28_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEO6815064.1"
                     /translation="MIIAIQGGMAVGKTTALKIIENVYPKIAISFEDISPVVDKINKH
                     QLDNNKLDDYCEIQQLFIQHERDRYELVRDNEIVLMDYGAEEIAFYTQYFPKVAGYSW
                     TLPSNLLLEVNRLKECFADYIIFLDADETVLYQRQKNDFNRQRHFFKWYTHHLLPAKK
                     HYLDTFSNVIHINTNHLNAEEVAEKIIDIIRKLTL"
     gene            8989..9870
                     /locus_tag="VBC28_001072"
     CDS             8989..9870
                     /locus_tag="VBC28_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000716337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-binding protein"
                     /protein_id="HEO6815065.1"
                     /translation="MKKHKMLSLLAVSGLMGIGILAGCSNDSSSSSKGTINIVSREEG
                     SGTRGAFIELFGIESKNKKGEKVDHTSDAATVTNSTSVMLTTVSKDPSAIGYSSLGSL
                     NSSVKVLKIDGKNATVKDIKSGSYKISRPFNIVTKEGKEKEATKDFIDYILSKDGQAV
                     VEKNGYIPLDNAKAYQAKVSSGKVVIAGSSSVTPVMEKIKEAYHKVNAKVDVEIQQSD
                     SSTGITSAIDGSADIGMASRELDKTESSKGVKATVIATDGIAVVVNKKNKVNDLSTKQ
                     VKDIFTGKTTSWSDLSK"
     gene            9885..10730
                     /gene="pstC"
                     /locus_tag="VBC28_001073"
     CDS             9885..10730
                     /gene="pstC"
                     /locus_tag="VBC28_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEO6815066.1"
                     /translation="MKQTKEGLMQAVFFITACVSVISAILICLFLFSSGLPAITKIGW
                     GNFIFGKVWHPSNNIFGIFPMIVGSLYVTAGALLLGGPIGILTAVFMAYFCPENIYKP
                     LKSAINLMAGIPSVVYGFFGLVVIVPMIRQYIGGFGMGVLAASILLGIMILPTIVSIS
                     ESSLRAVPESYYEGGIALGASHERSVFFAVLPAAKRGILASVVLGIGRAIGETMAVIM
                     VAGNQAVLPQSLTSGVRTLTTNIVMEMGYSSGLHRQALIGTAVVLFIFILMINISFSA
                     LQKEE"
     gene            10732..11610
                     /gene="pstA"
                     /locus_tag="VBC28_001074"
     CDS             10732..11610
                     /gene="pstA"
                     /locus_tag="VBC28_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEO6815067.1"
                     /translation="MENINQRTSKQYLASLKKDPLSFFLFAIVYLGAILSFATIAFVV
                     IYILVKGLPHVNTGLFAWTYNTQNVSLLPAFINTIFIIALTLLFAVPLGIGGSIYLTE
                     YARRDNPYLKIIRVATETLAGIPSIIYGLFGALFFVKYTHLGLSLISGSLTLSIMILP
                     LIMRTTEEALLSVPDSYREGAFALGAGKLRTIFKIVLPSAMSGIFAGIILAVGRIIGE
                     SAALIFTAGTVAKVAHSVFSSSRTLAVHMYAISGEGLYVDQTYATAVILLLLVIIVNF
                     VSGLVAKRLGSESNSE"
     gene            11603..12352
                     /gene="pstB"
                     /locus_tag="VBC28_001075"
     CDS             11603..12352
                     /gene="pstB"
                     /locus_tag="VBC28_001075"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEO6815068.1"
                     /translation="MSKLVINNLDLYYGEFHALKDVNLDIEEKEITAFIGPSGCGKST
                     LLKSINRMNDLVKNCKITGDITLEGEDVYRQLDINQLRKKVGMVFQKPNPFPMSIYDN
                     VAFGPRTHGIHSKAELDDIVERSLKQAALWDEVKDRLHKSALGMSGGQQQRLCIARAL
                     AIEPDVLLMDEPTSALDPISTAKIEELVIQLKKNYTIVIVTHNMQQAVRISDKTAFFL
                     MGEVVEYNKTSQLFSLPQDERTENYITGRFG"
     gene            12349..13005
                     /gene="phoU"
                     /locus_tag="VBC28_001076"
     CDS             12349..13005
                     /gene="phoU"
                     /locus_tag="VBC28_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000621378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEO6815069.1"
                     /translation="MIRSRFASQLNDLNKEIIFMGALCEDIIGKSLGALTNSNDVYLD
                     DISETYHKIEQMERDIEERCLKLLLRQQPVAKDLRRISSALKMVYDMKRIGAQAYEIA
                     EIVSLGHIIQGAGSERDQLNSMSNNVISMLTKSIDAFIYDNEEQAHQVIEQDRTVNQE
                     FDTIKKQLVLYFSVQDVDGEYPIDVLMIAKYLERIGDHTVNIAKWVLFSITGNLDGEA
                     "
     gene            13005..13682
                     /locus_tag="VBC28_001077"
     CDS             13005..13682
                     /locus_tag="VBC28_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001931721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO6815070.1"
                     /translation="MIYCVEDDADIREMMLYTLQMAGFKAQGFSSSELFWEAIQEKVP
                     DLILLDIMLPGDDGLTILERLRRKHQTEMIPVIMTTAKGSEYDKVKGLDLGADDYLVK
                     PFGMMEMISRIKAVLRRSRQVDSKAHIIIGNLEIDPTNYWVKRGTEKIHLTLKEFELL
                     VLFFRNPNRVFTRQELLDKVWGEQFLGETRTVDVHIGTLRTKLGEDGYLIATVRGVGY
                     RLEERHD"
     gene            13675..15330
                     /locus_tag="VBC28_001078"
     CDS             13675..15330
                     /locus_tag="VBC28_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEO6815071.1"
                     /translation="MTKKIFRTTLSASLGIVLVTILMIMGFLYNYFNHIQREQLRTQT
                     ALASQGISFEGKDYFENLKTSNVRITWVDNKGQVLYDTQSDAKHMKNHANRQEIKEAI
                     KSGYGESTRWSATLTEKSIYAAQRLNNGTIVRLSVAQQTIFYLLLGMISPLAIIILLA
                     IILSVLIARYIAKKVSEPLNNIDLDHPLSNDSYEEITPLLRRLDSHQAKIQHQKLLLQ
                     KRQKEFDTIISKIKEGMILLDDQARIVSINAEALKLFQINDDWHGRFMMEVSRDLTLK
                     DLIDQGLKGKKKEANIGIENNHYRVLVRPTTDNNRVTGLVVLLFDVTDQLQMEQLQRE
                     FTANVSHELKTPLHVISGYSELLANQMVPNEEVPQFAAKIHKESERLVKLVEDIINLS
                     HLDEQEKLPQETVNLYDLTQKVLEGLQAKADKKHIQINFNGEEAILRGNPVLLNSLVY
                     NLCDNAITYNHEKGQVNVTLKNSPDTITLEVSDTGLGIAEKDKKRIFERFYRVDKSRS
                     KIVGGTGLGLSIVKSALDFHNGSIKVDSHLGQGTTMTVLLHKQ"
     gene            15488..17671
                     /locus_tag="VBC28_001079"
     CDS             15488..17671
                     /locus_tag="VBC28_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000795069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEO6815072.1"
                     /translation="MKNNVKQLFSFEFWQKFGKALMVVIAVMPAAGLMVSIGNSISLL
                     DPSNVLLGRIANVIAQIGWGVIGNLHILFALAIGGSWAKERAGGAFAAGLSFILINLI
                     TGNFFGVKTDMLADSKATVQTVFGATIRVSDYFVNVLGQPALNMGVFVGIISGFVGAT
                     AFNKYYNYRKLPDALTFFNGKRFVPFVVIYRSVIVALILSVFWPVVQSGINGFGKWIA
                     SSQDSAPILAPFVYGTLERLLLPFGLHHMLTIPMNYTQLGGTYTVLTGATKGAQVLGQ
                     DPLWLAWVGDLINLKGSNSSQYHHLLTSVTPARFKVGQMIGASGILMGLSYAMYRNVD
                     KDKKLKYKSMFISAAAATFLTGVTEPIEYMFMFAAMPLYLVYAVVQGCAFAMADIVNL
                     RVHSFGNIEFLTRVPMGIKAGLGGDIFNFVWVTLLFAVLMYFIANFMIKKFNLATAGR
                     NGNYDNEEVDNAPSTASGSADANSQVVQVINLLGGRDNIEDVDACMTRLRVTVKDGNS
                     VGSEAAWKKAGAMGLVLKGNGVQAIYGPKADVLKSDIQDLLDSGTVIPIVDLETGQPV
                     AAAPVTTYKGITEEIVSVANGQVEALDVVKDPVFSQKMMGDGFAVEPTDGNIYVPVSG
                     TVTSVFPTKHAFGLLTESGLEVLVHIGLDTVALDGQPFEVKISSGQKVVAGDLAVVAD
                     LEAIKAAGKETSVIIVFTNVSDIKTVKLEKSGPQIAKTVVAKVEL"
     gene            17710..18540
                     /locus_tag="VBC28_001080"
     CDS             17710..18540
                     /locus_tag="VBC28_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEO6815073.1"
                     /translation="MKGYSMAKCLTLNTHSWMEVNALKKLFDLAEHIFREKYDIICLQ
                     EVNQSISSPLAKSSPNYHPIEGTPALHQDNFALQLVHYLNLQGLHYHWTWAYNHIGYS
                     KYHEGVAILSLKPLKPEDILVSAVDDETDYHTRRALVAETTLNDKVVTVVSLHFSWFE
                     KGFAEEWKRLETTLLEVETPLLLMGDFNNPTGNQGYELVLNSPLALKDSHQIANHVFG
                     DHTIMADIDGWEGNKKALKVDHIFTSEDLSISSSQVVFEGGEAPVVSDHYGLEITMDF
                     "
     gene            18817..19569
                     /locus_tag="VBC28_001081"
     CDS             18817..19569
                     /locus_tag="VBC28_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO6815074.1"
                     /translation="MNIFILEDDFVQQAHFEKIIKEIRVQYNLHFKTVETFAKPVQLL
                     ESIYEIGLHNLFFLDIEIKNDEQMGLEVAKQIRQVDPYAQIVFVTTHSELMPLTFRYQ
                     VSALDYIDKGLSQEEFSQRIEEVLLYVDGICNKPLVENSFYFKSRYSQVQLPFNDLLY
                     IETSSRSHRVVLYTEKDRMEFTATLGDILKQEPRLFQCHRSFLVNPLNIFKVDRIDRL
                     VYFQNGTTCLVSRNKVRDIVSIVDKYQKDRKR"
     gene            19638..19937
                     /locus_tag="VBC28_001082"
     CDS             19638..19937
                     /locus_tag="VBC28_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HEO6815075.1"
                     /translation="MANMIDYSHHIEVSLETDEPKIAMSYFTLNHKQIFIIENSIVEE
                     RIDASRLFGYGASSKGEDRGLGLYNVMKVIDRYPNASISTNSEQHTFRQGLEIIF"
     gene            20060..20908
                     /locus_tag="VBC28_001083"
     CDS             20060..20908
                     /locus_tag="VBC28_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000926579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6815076.1"
                     /translation="MLLQLDNITKSYGKKIVLNQISYQFTPGLYGLLGANGTGKTTLL
                     NLMSHFTLADSGNIYWNGQEQSEEFYRHIGFLPQHFRYYDQFTGIAFLNYIATLKGVD
                     KKKAKQEIPRLLELVGLGDVGKKKISSYSGGMKQRLGIAQALINDPEILILDEPTVGL
                     DPKERVKFRHILSQLSTNKIIILSTHIVSDVEAVAKEIIVLKNGKFIEHGNTAQLLKT
                     IEGKVWEITTEPGLSQIPNIAIVNEKVFSDSRVFRVVSDICPSDSAQLVVPTLEDFYI
                     YHFRGE"
     gene            20914..>21235
                     /locus_tag="VBC28_001084"
     CDS             20914..>21235
                     /locus_tag="VBC28_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6815077.1"
                     /translation="MISIFPFELKKLLQRKAAFGAFIIFLLAIFGLFYQHFFVGQISG
                     YSADKIHGREAVAINRRIAEKYSGDLSEERVTNIIKNYVNPPAGRPPIFDVFSYYVAD
                     TFDRD"
BASE COUNT         6681 a         3419 c         4452 g         6683 t
ORIGIN      
        1 taagaaagga gtaaaatggc aacatataga caaagaggta aaaagaaatt atgggattat
       61 agaatattca atgaaaaaag tgagttagtt gcttctggtt caggctttaa gacaaagcgt
      121 gaagctatga atgaagctat gaggatagag caacaaaagt tattagtaaa tgctatatct
      181 tctgatataa cattgtatga tttgtggttt gaatggtaca gtttaattat aaagcctagc
      241 aatctagcag agactacaaa gaacaaatat tttacaagag gttctgttat tcgtaaactt
      301 tttggaaatc agaaagtaaa taaaataaag catagtgcat atcagaggaa attaaatacg
      361 tacgctgaaa aatacactaa aaatcatgtc aggagattaa attctgatat aaaaaaagcc
      421 attcagtttg caaaaagaga tggcatttta ttatctgact ttacagatgg agttgtcata
      481 gctggtaggc aatttgttaa ggatgcagat gataaatatc tacatagtat ttctgattac
      541 aagaaagtga tatcttattt agaaaacaat ttgaattatt caaatagtat cgtttattat
      601 ttattactgg tgttatttaa aacaggttta cgagtgggtg aagctttagc attaacttgg
      661 gatgatgtta attttgaaga tctagaaatt aagacgtatc gaagatttag tggcgataag
      721 ggaactttta gtccacctaa aacaaaaact tctatccgca ctattcctat tagtcaatct
      781 ttagcattaa tcttaagaga tttgaaggat gatcagcaag ttatgttgaa aaacttaaaa
      841 attgtaaata ttaataatca aatattttat gactataggt acggtgtgtc gtcaaatagt
      901 gccattaata aaagtttgag aaatgtatta catttattaa atattgattc aaaaatgact
      961 gcaacaggtg cgagacatac ctatggaagt tatttattgg ctaagggtgt tgatatttgg
     1021 gtagttgcaa gattaatggg tcataaggat attacacagt tacttgaaac ttatggtcac
     1081 gtcttgacag aagtaataaa taaggaatat gaaacggtca gaagtttagt atcttaaagc
     1141 taatttttaa agaatatctt ctagaacaaa tttagaacaa atttgctgaa acaaagaaaa
     1201 aaacctttaa aatcaaggtt tttgacacta ttattaatgc cccctacagg gctcgaacct
     1261 gtgacccata gattaagagt ctactgctct accaactgag ctaaggaggc taggaagctg
     1321 tattggcacc gatgatccac tttttgtatt gatcccgcac aaaataagca ggtgggtaac
     1381 tcgcacctct ttaagtggct tccgcgtgtt aacggcttgc gcactagaga tgacttcgtt
     1441 tcccgaaata caaagaaata gtcggtcaac acttagatgt ggattcgtat gccacagcaa
     1501 tgtttctatg tctttatgat accacttttt ataaaatttt caagcaaaat tatcgatttt
     1561 tgaaatcgat tgcgattttt aaaactagca atcagtctga gagcttatca atgcgctctt
     1621 tatttgaacc aagtgcgcgt tcgtatttac cagtttcatt ggcttcaaag taattatgtc
     1681 ctactagttt atctggtaag tattgttgtt taacccattt ttcaggatag gcgtgagggt
     1741 acttgtaatc ccttgcattg cccaaggttt tactaccgct gtaatgtcca tctcttaggt
     1801 gacgtggaat aggtaaattt cctgaacgac gtaaatcagc taaagctgca tccatagcca
     1861 aataggctga atttgattta ggtgatagag ctaaatccac aacaatgtta gcaataagaa
     1921 tacgagcttc tggaaaacca atgcgttggg cagcttccaa tgcagtcaca gtatgtattt
     1981 gtgcttcggg gttagctaag ccgatatctt cataagcaat aatggtcaaa cgtcttgcta
     2041 aactaggtaa atctccggct tctactaaac gagcggcata gtgcaggctg gcattgacat
     2101 cagaacctct aattgatttt tgtaaggctg ataggatatc ataatgccca tctccgtttt
     2161 tatccattgt aatataacta cactgcagac tattttccat tgtctctaga ctaatatgac
     2221 ggctaccatc ttcattagga gaagtagaca tgactgctag atccaaggaa ttataagcag
     2281 atcgtaggtc tccatttgtg gcggtaacaa taaaatctag cgcctcatca tcaattgtca
     2341 ctaaaaaagg aaatccccgt tctttatcgg aaattgctag ctgaatggct tttttaatat
     2401 cctcgttaga aagaggttct aactcaaaaa tttgtaccct acttctaatg gcaggagtta
     2461 ctgaaaagaa aggattttca gttgttgctc caatcataat aatattgcca ttctcaagta
     2521 gaggtaaaag aaaatcttgt tttgtcttgt caagtctatg aatctcgtca agtaataaaa
     2581 ctaagccacc agaaaattta gcttcttctg caatttcttg aagacgtttt ttactatcaa
     2641 cagtggcatt aaaagtacga aaggcatatt tagtagtacc agcaatagca gaagcaatag
     2701 aagtcttccc aatacctggt gggccatata ggatcatgga agataacata ttagctgcaa
     2761 ccattcgatc aataattttg ccattaccaa cgagatgttg ctgcccgata acttcattaa
     2821 tattgcgagg acgcatacgt agagcaagat tgtcagccat gattattctc caaatctttt
     2881 gcgaaaatta aagcatcaac ataatgattt tcaatgaaaa aggctttagt gattcgagcc
     2941 tcttcggtaa acccgtattt ttgatagagg gaaatggctc tgctgttatt gcccatgaca
     3001 tgcatggcaa tcttgatgta accttgagct ttagcctcag ttagcagagc tttcaagaga
     3061 gctttaccca gtccctgacc ttggtagggt tcagcgatga gaatgccgaa agtggcaaca
     3121 tgcttgccag ctgggaaagg atagtaggga ccaaagtcca agacgccagc gatcttatca
     3181 tcgacctcag cgacaaagaa ggacatgcca tttgccagat tcttctccaa atcactctca
     3241 gtccacttgc ggtcatagac aggagaggta agagcagtcc agccagtatt ttgtaaggtc
     3301 agaactgcct cacggtcgca gtcctgcata ggtctgataa tcataattga tgatacaaag
     3361 ggacggttcg gtttaccgaa aattttcgta aagaaatagg gaacgacgaa gtgtcgtcag
     3421 acattaggaa gttcatcttt tcttctagaa aattagtccc gtattcaaat cctttctatt
     3481 ttctatatag agtttttctc ttggctgacg cttttaacat agtatgaagt cagtcaaaaa
     3541 cggctagtct ttgagactat tgtaacataa aaataagtag gaactacctt ttgattaacc
     3601 tgagtctgta acgtttttct tctatttcat gttataataa gaaagaataa atagaaaata
     3661 aggacataag atggctaaat ttggattttt atcggtacta gaagaagaat tggataagca
     3721 tttacagtat gactttgcca tggattggga taagaaaaat catactgtag aagtcacttt
     3781 tatattagaa gcacaaaaca gctctgctat tgaaaccgtg gacgaccagg gggagacgag
     3841 tagcgaggac atcgtttttg aggactatgt actcttttac aaccctgtca agtctcgctt
     3901 tgatgctgag gactatctgg tgactatccc ctatgagcct aagaagggct tgtcgcgtga
     3961 gtttctggct tattttgcgg agactctcaa cgaggttgcc actgaagggt tgagcgacct
     4021 catggacttc ctcaccgatg acagtatcga agagttcggt ctttcttggg atacggatgc
     4081 ttttgaaaat ggcagagcag aactgaaaga gacagagttt tacccttatc cgagatatta
     4141 gacttgatgg atttgctaaa atgtggatat acattggatg atatagaaac agccagacca
     4201 gaggatatgg aacgttatta tgcccctgaa caaatcagaa aatatggagc tttaggaata
     4261 gaattgaggt tattgcatgg atatttttga tgagttattt gatttctcag gtgcaaagat
     4321 cgctcttttc tgcgagggaa agattttgac cagcctaaga gatgattttc cagatttgcc
     4381 ctatgcaggc ttttgggact taccaggcgg tggtcgtgag gacaatgaaa cacctttgga
     4441 atgtcttttt cgtgaagttg atgaagaatt aagtctgacc ttaactcgaa accatattga
     4501 ttgggtcaag acttataggg gtatgttgaa gcctgataaa ttatcagttt ttatggttgg
     4561 acatatcagt cagaaagagt acgatagcat tgttctaggc gatgaaggac aagactacaa
     4621 actcatgtcc attgacgaat ttttaagcca taaaaaggtc attcctcagt tgcaggagag
     4681 gttgagagat tatttggagg tggaagatga ttactttaca aaaggcggaa gctagtgatt
     4741 tagagaagat tattgccatt cagagagcta gctttaaggc agtctatgag aaataccacg
     4801 atcaatacga tccctatgtg gaagaagttg agcagattcg atggaaacta gtggagcgac
     4861 cagattgttt ctatcatttt gtccttgttg atgagacaat tgttggtttt cttagattgg
     4921 ttatcaaaga cgaggagaag cgtgcttggc ttggtacggc ggctattctt ccccaatatc
     4981 aagggcaggg ctacggctca gcagcgatgg ctttattaga gaaaacctat cctaagctta
     5041 caaagtggga tttatgtacg attgcgcaag aaaaactaat ggtcagtttt tatgaaaaat
     5101 gtggttatca ttcaacccat accgaacctg agcaagaagg aatggatatg gtctatatga
     5161 caaagaagat agaataaaaa aagaaaacta gatgattgtc tagttttctg atagtttatt
     5221 ggcaatagta agcaatagct tggctgacaa attcacttgt tcctgctcca ccagctttgt
     5281 caattgattg actaaaccgg tcatctgatt gatacatttg tcctagtcca gcaaggattt
     5341 cattggtaca ggtgtaaaaa tgctgagaga tatagtcttg tagccgtttc acacacattt
     5401 gaacagattc atcagataca ttttgtgtct ttaattgccc aaattgtacc attatttttg
     5461 ccatttcttg gctaatttgt gcaaagtcat ctgaagcatg tttttgagca aattctttgt
     5521 aggctgctgt ttgtccccac ttttcttttg cttcagcttg taattgttct aattctgttt
     5581 tattataaac atcgaaattc atataatcat ctcctttttc ttgtaatcgt ttcgcatggg
     5641 tgataacctg ctcaaggtgc gatcttttcc attcaagcaa tttgatttgt tgggctaata
     5701 agtcttgcct ttcctctttt gcttgctcaa gcaggtattt gatatctttg agagggaatt
     5761 ccaactcacg aaatagcaaa atttcttgca ggcgaatcaa tgcttcatcg tcataaagtc
     5821 gatagcctgc ttcggatagg gcggtaggtg ggaataaacc gattttatca tagtgatgca
     5881 aggtgcgcac acttacaccg cttaagatac tgacttcttt aactgttttc atggtaataa
     5941 cctcctctca cttacatagt ataaaggata acctaaggtc agggtcaagc aaaaaataaa
     6001 ttattttgaa aagtatggtt aatcatattg atttgaagat aaaggaggga tagttataga
     6061 aatagttgag ctacttaggg aatggtattc ttcctttgaa gttaaagaag aaaagtggaa
     6121 tgaaaaatat gaaggtatcc tcataagaat tgaaggcgat ggaaactata aaaggtgtcg
     6181 tttagctcat aagacagcta gcaagatggg gtattttaca gtattttggc agaaagatgg
     6241 aaatggtaaa aatattcctt tttatgaaca agatgaagtg gaagatttga ttattgtcat
     6301 taaagatggt aggtggaaag gtttgttcat cattcccaaa gaggttgcag tgagtaaggg
     6361 tattctttca tctgctaact ctcaaggaaa gatggctatg cgattttacc ctccttggtg
     6421 tagcgattta aatagaacag ctttggtgac acagcgatgg cagttgaact attttattga
     6481 tttaagtaga aataatgaag gagttactac atgaacacat ggaatgaact aacggttcac
     6541 gtgaatcgtg aggcagagga agctgtctcg aatttattga ttgaaactgg cagtcagggg
     6601 gttgccatta gcgattcggc tgactacctt ggacaggagg atcgtttcgg tgagctttat
     6661 cctgaggtgg agcagtctga tatgattgct attactgctt attatcctga tacgcttgat
     6721 attgaggcgg ttaaggctga tttggctgat cgtttagcta attttgaagg ctttggtctt
     6781 gcgacgggta gcgtcaacct tgacagtcag gaattggtcg aagaggactg ggctgataac
     6841 tggaagaagt actatgagcc agctcgcatt acccatgatt tgaccatcgt gccgtcatgg
     6901 acggactacg aggctaaggc tggtgagaag attatcaaga tggatcctgg tatggctttt
     6961 gggactggga ctcacccaac gactaagatg agtctttttg ccttggagca agtgcttcgt
     7021 ggtggtgaga cggttatcga tgtggggact ggttctggcg ttttgtctat tgctagctct
     7081 ctcttgggtg ccaaggacat ctatgcctat gatttggatg atgttgcagt gcgcgtggct
     7141 caggaaaata tcgatatgaa ccctggcact gaaaatattc atgtggctgc tggtgatctg
     7201 ctcaaaggtg tccagcaaga ggtagatgtt attgttgcca atattttggc ggatattttg
     7261 atccatctga cagacgacgc ctaccgtttg gtcaaggatg agggctatct catcatgtct
     7321 ggtatcattt ctgagaagtg ggacatggtg cgtgagagtg ctgaaaaagc tggtttcttc
     7381 cttgagactc acatggttca aggtgagtgg aatgcctgcg ttttcaaaaa gacagatgat
     7441 atttcaggag tgattggcgg ctaatgcaac agtattttgt aaatggcgaa gcaggtgcct
     7501 atgtgaccat cgaggacaag gataccatca agcacatgtt caatgtcatg cgattgacag
     7561 aagatgacca agtggtcttg gtttttgatg atgccatcaa gcgtctggca aaggtggtgg
     7621 acagctctgc tcatcgcttc caaattctag aagagctaga caacaatgtt gaaatgccag
     7681 ttcaagtgac cattgcctca ggttttccca agggagacaa gttggatttt gtgacgcaaa
     7741 aggcaacaga attgggtgct gcggctatct ggggattccc agctgactgg tcagtcgtca
     7801 agtgggacgg caagaaacta gctaaaaaag aagataagct ggcaaaaatt gctctgggcg
     7861 cagcggagca gagcaagcgt aatcgtcttc ctcaggttcg tttatttgag aagaaggctg
     7921 attttcaagc ggagcttgca ggctttgata agatttttat cgcctatgaa gagtctgcca
     7981 aggagggtga attgtcagcg ctagcccaga acttgcagac tgtaaaagca ggtgacaagc
     8041 tcctcttcat ctttggacca gaagggggca tttctccaaa agaaatcgca gcttttgaag
     8101 aggttggagc tatcaaggtg ggcttggggc cacgcatcat gcgcacagaa acagcgccgc
     8161 tctatgctct aagtgttatt agttactcag cggagttatt atgattattg ctattcaagg
     8221 tggtatggct gtagggaaaa ccacagcttt gaaaatcatt gagaatgttt accccaaaat
     8281 cgctattagt tttgaggata tttctccagt agttgataag attaataaac atcagcttga
     8341 taacaataaa ttagacgatt attgtgaaat tcaacaacta tttattcagc atgagcgaga
     8401 tagatacgaa cttgttagag ataatgaaat agtgttaatg gattatggag ctgaggaaat
     8461 tgctttttat acacaatact ttccaaaggt ggcgggttat agttggactt taccttctaa
     8521 tttgttacta gaggtaaata ggctaaagga atgttttgct gattatatta tttttctaga
     8581 tgcagatgag acggtcctat accaacgtca aaaaaatgat tttaatagac agcgtcattt
     8641 tttcaaatgg tatactcatc acctgctgcc tgctaaaaaa cattatttag acacattttc
     8701 caatgttatt catattaata caaatcattt aaatgctgag gaagtggctg aaaaaatcat
     8761 tgacatcatt cgtaagttaa ctctataatg ggatagttat actccaatta tgtgatgtaa
     8821 caactgtttc aatccgactt actggctgaa ttaccataat agaaaaaagg agtttggata
     8881 attttccaac tctttttaat tttacattga ttttacaaaa atcctcaaag agatttgata
     8941 tttcttaact ataataaaat caaatcaaaa aatatttagg agttcatcat gaaaaaacac
     9001 aaaatgctat ctcttttggc ggtatctggt cttatgggaa ttggcatcct agcaggatgc
     9061 tcaaatgatt catcatcttc atctaaaggg acaattaaca ttgtttctag agaggaaggg
     9121 tctggaactc gtggagcttt catagagtta tttgggatcg agtcaaaaaa taaaaagggc
     9181 gaaaaagtag accatacttc tgatgcagca actgttacca attcaacttc agttatgcta
     9241 accactgtct ctaaagaccc gtcagctatt ggttattctt cactaggttc attaaatagt
     9301 agcgttaaag tgcttaaaat tgatggcaaa aatgcgactg taaaagatat taaatcagga
     9361 agctataaga tttctcgtcc tttcaatatt gtaacaaaag aaggtaagga aaaagaagca
     9421 acaaaagatt ttattgatta tatcttaagc aaagatgggc aagcagtcgt tgagaaaaat
     9481 ggctatattc cacttgacaa tgctaaagca tatcaagcaa aagtgtcatc tggtaaagtt
     9541 gttatagcag gttcaagttc agtaacgccg gttatggaaa agattaaaga agcctatcat
     9601 aaagtaaatg caaaagtaga tgttgaaatt caacaaagtg attcttcaac tgggattaca
     9661 agcgcaattg atggatcagc agatataggt atggcatcac gtgaattgga taagacagag
     9721 tcatccaaag gggtcaaagc tactgttatt gcaacagatg gtattgcagt agttgttaat
     9781 aagaaaaata aagttaatga tctatcaaca aaacaagtaa aagacatttt tactggaaag
     9841 acaacgtctt ggtctgactt atcaaagtaa ggtaagagta aacaatgaaa caaacaaaag
     9901 aaggtcttat gcaagctgtg tttttcatca cggcttgcgt cagtgttata tcagctattt
     9961 taatttgcct ctttctcttc tctagtggtc ttccagctat tacgaaaatc ggctggggaa
    10021 attttatatt tggtaaagtc tggcatccta gtaataatat ttttggcatc tttccaatga
    10081 ttgtaggttc cttatatgtt acagcgggag ctttactttt aggaggaccg ataggtatcc
    10141 ttactgctgt ttttatggct tatttctgcc cagaaaatat ttataagcct ttaaagtcag
    10201 ctatcaattt aatggcggga atcccttcag ttgtttatgg tttctttgga ctcgttgtta
    10261 ttgtccctat gataaggcaa tatattggtg gctttggtat gggagtctta gcagcttcaa
    10321 ttttgctagg aattatgatt ttaccaacaa ttgttagcat ttcagaatct tctttaagag
    10381 ctgttccaga gagctattat gaaggcggta ttgcccttgg tgcctctcat gaacgaagtg
    10441 tcttctttgc tgttttacca gctgctaaac gtggcatttt agcatcggtt gttttgggaa
    10501 ttggacgtgc tattggtgaa acaatggctg tcatcatggt agcgggtaat caagctgtct
    10561 tgcctcaatc gctgacatca ggcgttagaa ctctaaccac taacattgtt atggaaatgg
    10621 gctattcttc tggtttgcat cgtcaagcct taattggtac agctgtggta ctctttatct
    10681 ttatattaat gattaatatc agtttttcag cattacagaa agaggaataa gatggaaaat
    10741 attaaccaaa gaacgtcaaa acaatattta gcatctttaa aaaaagatcc cctttccttc
    10801 tttctttttg ctattgtcta tttgggagca attctaagtt ttgctacaat tgcatttgtt
    10861 gttatttata ttttagttaa ggggcttcct catgtgaata ctggcttgtt tgcatggact
    10921 tataatactc agaacgtttc actattacca gctttcatta acactatctt tatcatcgcc
    10981 ttaactttat tatttgcagt tccacttggt attggtggat caatttatct aacggaatat
    11041 gctagacgtg ataatcctta tctcaaaatt atccgtgttg caaccgagac attggcaggg
    11101 ataccttcaa ttatttatgg tttgtttggt gcattatttt ttgtaaaata cacacactta
    11161 ggcttatctt tgatatcggg ttctctgact ttgagcatca tgattttacc tttgattatg
    11221 aggacaactg aagaagcttt actatctgtt ccggattctt atcgagaagg agcctttgcg
    11281 cttggtgctg gtaaattgag aaccatattt aaaatcgtat tacctagtgc tatgtctggt
    11341 atttttgctg gtattatttt agcggtagga cgtatcattg gtgagtcagc agcacttatt
    11401 ttcacagctg gaacagttgc aaaagtagca cattcggtat ttagttcatc tcgtacttta
    11461 gcagtacata tgtatgctat ttcgggtgag ggcttatatg ttgaccaaac ttatgcgact
    11521 gcggtcatct tattgctttt agttattatt gtcaattttg tatctggact agtagccaaa
    11581 cgattaggaa gtgagtcgaa cagtgagtaa attagtaatt aataatttag atttgtatta
    11641 tggagaattt cacgctctga aggatgttaa tttagacatt gaagaaaaag aaatcacagc
    11701 ctttattgga ccttctgggt gcggaaagtc tactctcttg aaaagtatta accgtatgaa
    11761 tgatttggtc aaaaattgca aaatcacagg ggatattact ttagaaggcg aggatgtcta
    11821 tagacaattg gatattaacc aattgcgtaa aaaggttgga atggtgtttc aaaaacctaa
    11881 tccgtttcca atgagcattt atgataatgt tgcttttggt ccaagaacgc acggtattca
    11941 ttccaaagca gagttagatg atatcgttga acgttccctt aaacaggcgg ctctatggga
    12001 tgaggttaaa gatcgtcttc acaaatctgc tctgggtatg tctggcggac agcagcaacg
    12061 tttgtgtatt gcgcgtgctc ttgctattga gcctgatgtt ttacttatgg atgaacccac
    12121 aagtgccttg gatcctattt caacagcaaa aattgaagaa ttggttatcc aactgaagaa
    12181 gaactacact attgttatag tgacacataa tatgcaacaa gctgttcgta tttctgataa
    12241 aacagcattc tttctgatgg gagaagtggt tgaatataac aaaacgagcc aactcttttc
    12301 attgccacaa gatgagcgta cagaaaacta cattacaggg agattcggat gattcgttca
    12361 cgatttgcaa gtcaacttaa tgaccttaat aaagaaatta tttttatggg ggcattgtgt
    12421 gaggacatca ttgggaagtc cttaggtgct ctaacaaata gtaatgacgt ttaccttgac
    12481 gatatttctg aaacttatca caagattgaa caaatggaac gggatattga ggagagatgt
    12541 ttaaaacttt tactccgtca gcaaccagtt gccaaggatt tacgccgtat ttcttctgca
    12601 ttaaaaatgg tttatgatat gaaacgtatt ggtgctcaag cttatgaaat tgcggaaatt
    12661 gttagtttag gtcatattat ccaaggtgca ggttctgaaa gggatcaact taacagcatg
    12721 tctaataatg tcattagtat gctgactaag agtatagatg cttttattta tgacaatgaa
    12781 gaacaagcgc atcaagtaat tgaacaagat aggacagtta accaggaatt tgatacaata
    12841 aagaaacagt tagtgttata tttttcggta caggatgttg atggtgaata tcccatagat
    12901 gttttaatga tcgccaagta cctagagcgt attggtgatc atacagtaaa tattgctaag
    12961 tgggtgcttt tctcaataac aggtaaccta gatggagaag cgtaatgata tattgtgttg
    13021 aagatgatgc tgatattcgc gaaatgatgc tctatacttt gcaaatggca ggttttaaag
    13081 ctcaaggatt ttcgagttca gagctttttt gggaagctat tcaggagaaa gttccagact
    13141 tgattttgct tgatattatg ttgccgggag atgatggttt gactatttta gagcgtttaa
    13201 ggagaaaaca tcaaacggaa atgataccag taatcatgac aactgctaaa ggtagtgagt
    13261 atgataaagt taaagggtta gatcttggag cagatgatta tctcgtcaaa ccttttggga
    13321 tgatggaaat gatttcacga ataaaagcag tcttgagacg tagccgccaa gtagattcaa
    13381 aagctcatat tatcattgga aatttagaga ttgatccgac taattattgg gtcaaaaggg
    13441 gaacggaaaa aatccactta accctaaagg aatttgagtt attagtatta ttcttccgta
    13501 atcccaatag agtctttaca aggcaagaac ttctggataa ggtttgggga gaacaatttt
    13561 taggagaaac tagaactgtg gatgttcaca ttgggacact gcgaacaaaa cttggtgagg
    13621 atggctattt gattgctaca gtaaggggag ttggataccg tttggaggaa agacatgact
    13681 aaaaaaattt tccgtacaac tttatctgct agtttgggaa ttgttttagt tacaattttg
    13741 atgattatgg gatttctcta taattacttc aatcacattc agcgagaaca gttgagaaca
    13801 caaacggcac tggcgtcaca aggaatttcc tttgaaggta aagattattt tgaaaatctt
    13861 aaaacttcaa atgtgcgtat tacttgggta gataataagg gacaagtgct ttatgatacc
    13921 caatcagatg caaaacatat gaaaaatcac gccaatcgtc aagaaattaa agaagctatc
    13981 aagagtggtt atggtgagag cacgaggtgg tcagcgacac ttactgagaa gtctatctat
    14041 gctgcgcaac gtttaaataa tggaacgata gttcgtttat cagttgcgca acaaactatt
    14101 ttttatcttc tccttggtat gataagtccc ttagcaatta tcatcttact tgctattatt
    14161 ttatctgtat taattgcacg atatatagca aaaaaagtat cagagccttt aaacaatatt
    14221 gacttagatc atcctttatc aaatgatagt tacgaggaga taacaccatt attaaggcgt
    14281 cttgatagcc atcaggctaa gattcaacat caaaaactgt tattacaaaa gcgtcaaaaa
    14341 gaatttgata ccattatttc taaaattaag gaaggaatga ttcttttaga tgatcaagca
    14401 agaatcgtta gtatcaacgc agaagcttta aagttattcc aaattaatga tgattggcat
    14461 gggcgcttta tgatggaagt tagccgagat ttaactctca aagacttaat cgatcaagga
    14521 ctaaaaggga aaaagaaaga ggctaatatt ggcattgaaa ataatcatta ccgcgtcttg
    14581 gtaagaccaa ctacagataa caaccgagta acaggattgg ttgttctact gtttgatgtg
    14641 actgatcaac tccaaatgga acaacttcaa cgtgaattta ctgccaatgt ctctcatgaa
    14701 ttgaaaaccc cgcttcatgt tatttctggt tactcagaac tgttagctaa tcaaatggtt
    14761 ccaaacgaag aagtcccaca atttgcagct aaaattcata aagaatctga acgattagtt
    14821 aaattggttg aggatattat caacctttct caccttgatg aacaagaaaa attaccgcaa
    14881 gagacagtaa atctgtatga cttaacacaa aaagttttag aaggtttaca agctaaagca
    14941 gataagaagc acatacaaat caattttaat ggagaagaag ctattttgcg agggaatcca
    15001 gttttattga attccttggt gtacaacttg tgtgacaatg ctatcaccta taaccatgag
    15061 aaaggtcaag taaatgtgac tttgaaaaat tcacctgata caattacctt agaagttagt
    15121 gatacgggac taggcattgc agagaaggac aaaaaacgta ttttcgaacg tttctatcgc
    15181 gtcgataaaa gtcgctcaaa aatagttggg gggactggtt taggtttgtc tattgtaaag
    15241 agtgctttag attttcacaa tggtagtata aaagtggaca gccatttggg gcagggaaca
    15301 acaatgaccg ttttacttca caaacaataa aaactcacaa ataaaagtct agatgatatt
    15361 atctagactt ttttagtaat tcagaaaaaa tatataagta agggtttaca aaaaacaaat
    15421 aagtgctata ataaaaccat aataacgcaa acgattgcac tattaagtgc ataaaaggag
    15481 attgaagatg aaaaataatg tcaaacagct ctttagtttt gaattttggc agaagtttgg
    15541 gaaggctttg atggttgtta ttgctgttat gccagcagca ggattgatgg ttagtattgg
    15601 taactccatt agtctattgg atccatcaaa tgttttatta gggcgcatag ctaatgttat
    15661 tgctcaaatt ggttggggag ttattggtaa cttacacatc ttatttgcat tggctattgg
    15721 tggtagctgg gctaaagaac gtgccggtgg tgcttttgcg gctggcttat cctttattct
    15781 tattaacctt attacaggga acttttttgg tgttaaaaca gatatgttag cagacagtaa
    15841 agcaactgtt caaacggtat ttggtgcaac catcagagtt tcagattact ttgttaacgt
    15901 tcttgggcaa cctgctctta atatgggtgt atttgtcggt attatttcag gtttcgttgg
    15961 tgcaactgcc tttaataaat attataacta tcgtaaatta ccagatgctt taactttttt
    16021 caatggaaaa cgttttgttc catttgttgt tatttatcgt tcagtcattg ttgcattaat
    16081 tttatcagta ttttggccgg ttgttcaatc aggtatcaat ggctttggta aatggattgc
    16141 aagttcacaa gacagtgctc caatattagc accatttgtc tacggtacgt tagagcgttt
    16201 attgctccca tttggtttac accatatgtt aactattcca atgaactaca cgcaactagg
    16261 tggcacatac actgtcctta caggtgcaac taaaggagca caggtgttgg gacaagatcc
    16321 actgtggtta gcatgggttg gtgatttaat taacttaaaa ggctcaaatt caagtcaata
    16381 ccatcactta ttgacaagtg tcactccagc acgttttaaa gtggggcaaa tgattggtgc
    16441 ttcaggtatt ttgatgggat tatcttatgc tatgtatcgt aacgttgata aagataaaaa
    16501 attgaaatac aagtcaatgt ttatttcagc agcagcggct actttcttga caggtgttac
    16561 tgagccaatt gagtacatgt tcatgtttgc agcaatgcca ctctaccttg tatatgctgt
    16621 cgttcaagga tgtgcgtttg ctatggcaga tattgttaac ctccgcgttc attcatttgg
    16681 taatatagaa tttttaacac gtgttcctat gggcataaaa gcgggccttg gtggtgatat
    16741 tttcaacttt gtttgggtga cacttctttt tgcagtattg atgtacttta ttgcaaactt
    16801 tatgattaaa aaattcaatt tagcaacagc aggacgtaat ggtaattatg ataacgaaga
    16861 ggttgataat gctccttcaa cagcttctgg ttctgctgat gcaaattctc aagttgtaca
    16921 agttattaat ttacttggtg gtcgtgataa cattgaagat gtagatgcat gtatgactcg
    16981 tcttcgtgtc acagttaaag atggaaatag cgtgggttct gaagcagctt ggaaaaaagc
    17041 tggtgcaatg ggattggtgt taaaaggtaa tggcgttcaa gctatttacg gacctaaagc
    17101 agatgtcctt aaatcagaca ttcaagattt gctagattca ggtacagtta ttccaatagt
    17161 tgatttagaa acaggtcaac ctgtagcagc agccccagtt acaacttata aaggaataac
    17221 agaagaaatt gtttcggtgg ctaatggtca agtagaagca ttggatgttg ttaaagaccc
    17281 tgtcttttct caaaaaatga tgggtgatgg ctttgctgtg gaaccaactg atggcaatat
    17341 ttatgttcca gtttcaggta cagttactag tgtctttcca acaaaacatg cttttggttt
    17401 attaacagaa agtggcctag aagttttagt acatatcggt ctagatacag tagctcttga
    17461 tggtcaacca tttgaggtta aaatttctag tggtcaaaaa gtggtagcag gcgatcttgc
    17521 tgtagtagca gatttggaag ctatcaaagc agctggtaaa gagacaagtg tgattatcgt
    17581 ctttacaaat gtttcagata taaaaactgt taaacttgaa aaatcaggtc cacaaatagc
    17641 taaaacagtt gtggcaaaag tcgaactgta acaaacgata ggagctgggc aagtctcagc
    17701 ttctttctga tgaaaggata ctctatggca aaatgcttaa cgttaaatac tcattcatgg
    17761 atggaagtaa atgctctaaa gaaattattt gatttagcag agcatatctt ccgtgaaaaa
    17821 tatgatatca tctgcctaca agaggtgaac cagagcatat cttcccctct tgctaagagt
    17881 agtccaaatt atcatccgat tgaaggtaca ccagctcttc atcaagataa ctttgccttg
    17941 caattagtgc attacctaaa tcttcaaggt ctacactatc attggacatg ggcttataat
    18001 catattggtt atagtaagta ccatgaaggg gtagcgatac tttctctgaa accattaaag
    18061 ccagaggata ttttagtttc agctgtagac gatgagactg attatcatac tcgtcgtgct
    18121 ctggtagctg agaccactct gaatgacaaa gtcgttacag tagttagcct ccatttctca
    18181 tggtttgaga aaggatttgc ggaggagtgg aaacgactag aaactaccct acttgaggta
    18241 gaaactccac ttttactcat gggtgacttt aataatccta ctggaaatca gggttatgaa
    18301 cttgttctta atagtccatt agcattaaag gatagccacc aaatagccaa tcatgtattt
    18361 ggggaccata ctattatggc tgatattgat ggttgggaag ggaataaaaa ggctcttaaa
    18421 gttgaccata tttttactag cgaagacctt tcaatcagtt caagtcaagt agtttttgaa
    18481 ggtggtgaag cacctgtcgt tagtgatcat tacggcttgg aaattaccat ggatttctaa
    18541 tatcttataa taaaagatac ccttaggttc agataaagtc tgtatctaag ggtattttaa
    18601 tagaaataat ttatttttaa tacttaacta cttaattagg tgttcaaata gttatatata
    18661 tgttaaaatt agcatatatt tattatgctt ttaaaattag cctcatggac gtcacatatt
    18721 aagacgggat aactcataaa cgatacctat gagaagaata tcgtagtggc tctattgtga
    18781 ctattaatat tcaagtaata agatagagga tataaggtga atatttttat acttgaggat
    18841 gattttgttc aacaagccca ttttgaaaaa atcattaaag agattagagt acagtacaat
    18901 ctgcatttca aaacagtaga aacgtttgct aaaccagttc aattattgga atcaatttat
    18961 gaaataggtt tacataattt atttttctta gatattgaaa taaaaaatga tgagcagatg
    19021 ggcttagagg tagcaaaaca aattagacaa gttgatccat atgctcagat tgtttttgtt
    19081 acaactcatt cagaattaat gcctttaacc tttcgttatc aggtatcagc tttagactat
    19141 atcgataaag gactttcaca ggaagagttt agccaaagga ttgaggaagt attgttgtac
    19201 gtagatggca tttgcaataa gcccttagtg gaaaattcat tctactttaa gtcgcgttac
    19261 agccaggtac aattaccttt taacgatctt ctttatatag aaacatcctc aagatcacat
    19321 agagttgtcc tttatactga aaaagataga atggaattca cagccacttt gggggatatt
    19381 ttaaaacaag aacctaggct ctttcaatgt catcgctctt ttttagtaaa tcccttaaat
    19441 atttttaaag tggataggat agatagatta gtttacttcc aaaatggaac gacttgtctg
    19501 gtttcacgca ataaagtgag agatatagta tctatagtgg ataaatatca aaaggataga
    19561 aaaagatgag ttctatttta gaccgttact tcaaaacttc tttacaggag aggttggttg
    19621 ttcaaagaaa actctaattg gctaatatga tagattatag ccaccatatt gaagtttctt
    19681 tagagacaga tgaacctaag atagccatgt cttattttac attaaaccat aagcaaattt
    19741 ttatcataga aaattcgata gtcgaggaaa gaatagatgc ttcacgtttg tttggttatg
    19801 gagcaagtag caagggagaa gatagagggt taggccttta taatgtcatg aaagttattg
    19861 accgttatcc caatgcatct attagtacaa atagtgaaca acatactttc cgtcagggct
    19921 tagaaataat cttttagcta tattacttta caatcattta gagagtaagt gagaaagctc
    19981 atttttaacc tttagctcag tcgctatatt ttagtaattg atttttcaac tgtcttaatt
    20041 attgaaagga gacaatacta tgttattaca gctagataat atcactaaaa gctacggaaa
    20101 aaaaatagta cttaaccaga tttcctacca gtttactcct ggtttgtatg gactcttagg
    20161 agcaaatggt actggcaaaa caaccttatt aaatttgatg agccacttta cattagcaga
    20221 tagtgggaat atttattgga atggacagga gcagtcggaa gaattttatc gtcacattgg
    20281 ttttttaccg caacattttc gctattatga tcaatttact ggcattgctt tcttaaatta
    20341 tatcgctacc ttaaaaggtg ttgataaaaa gaaagctaaa caagaaattc ctagattact
    20401 agagcttgtt ggtttaggcg atgtaggtaa aaagaagatt tcatcatact ccggtggtat
    20461 gaagcaacgt ttaggcattg ctcaagcact tatcaatgat cctgaaatac tcattctgga
    20521 tgaaccaact gttggacttg atcccaagga acgggttaaa tttcgccata ttttgagtca
    20581 gttatccacc aataaaatta tcattttatc aacgcatatt gtgtcggatg tggaagctgt
    20641 tgccaaagaa attatagtat taaaaaatgg taaatttatc gaacatggca acaccgcaca
    20701 gctgttgaaa actattgagg gaaaagtgtg ggaaataacg actgaaccag gtttatctca
    20761 gatacccaat attgctattg ttaatgaaaa agtcttttca gatagtcggg tatttcgtgt
    20821 agtgagtgat atttgtccga gtgattcggc acagttggtt gttccgactc tagaagattt
    20881 ctacatctat cattttagag gggagtaagt aagatgatat caatttttcc ttttgaatta
    20941 aaaaaacttc tacaacgtaa ggcagcattt ggggctttta ttatcttcct tcttgctatt
    21001 tttggtctat tttaccagca tttttttgtt gggcaaatta gtggctattc tgcagataaa
    21061 atacatggtc gagaagcggt tgccattaat cgtagaatag ctgaaaaata ttcaggggat
    21121 ttatctgaag aacgagtaac aaatattatt aaaaactatg ttaatccgcc agcaggacgc
    21181 ccccctatat ttgatgtttt tagttattat gtggcagaca cttttgatcg tgatt
//
LOCUS       DATRMA010000031        20485 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.031, whole genome shotgun sequence.
ACCESSION   DATRMA010000031 DATRMA010000000
VERSION     DATRMA010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20485
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.031"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            complement(<1..743)
                     /locus_tag="VBC28_001085"
     CDS             complement(<1..743)
                     /locus_tag="VBC28_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEO6815078.1"
                     /translation="MAKIISLGEVLLRLSPPQYHTLMQANHLKCQFGGSELNVLASLA
                     QLGYHVGLVSALPDNDLGKMASQFILSQQISPAAIIKKEGRLGIYYYEQGFSVRTNKV
                     IYDRNYSSFWESTLSDYDFTSIFKGVDWFHVSGITPALTKDLYEVTRFLMTKAKEGGV
                     KVSIDLNFRESLWSSFQEAREQLSPLLGLLDVCFGLEPIYLAGESEDLKDELGLSRPY
                     LDIELLEKITQKIVQEYGLDYIAFTQREM"
     gene            complement(810..2360)
                     /locus_tag="VBC28_001086"
     CDS             complement(810..2360)
                     /locus_tag="VBC28_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HEO6815079.1"
                     /translation="MEKTMDMEQREEVVSYNRAKVWQLVMFAMNNASTNIYLFTFMFV
                     TYFSTGVLGSAAIFVSQIMGYIRIFDGFIDPAIGIMIDKTDTKFGKYRPILIIGNVIT
                     ALSLIFLLALRGVDENIRFPLFILVLIIHKIGYSMQQTITKAGQTALTNDPKQRPIFN
                     IVDAVMTTSLMTGGQFVVSVFLVPKFGNFTPQFFNVLIFGTILISAILAIVAIIGIWA
                     KDRKEFFGLGENTQKTALKDYWKVLKGNKPLQILSIAAALVKFAIQFFGDSVVMVLLF
                     GILFGNYALSGQFSLLFIVPGVIINILFSTIARKKGLRFSYVRAIQIGMIGLLAFGAV
                     LYVGKPGDLSLTSLNLYTILFIVTNIIARYASQAPASLVLTMGADISDYETSESGRYV
                     SGMIGTIFSLTDSIASSFAPMVVGFVLAGIGFSKSFPTIETPLPPDLKMAAISILVAI
                     PFIALSIALLLMKFYKLDKEEMVRIQEKIQVMKAATTKERAKDIARNVPLTDMDYVDV
                     TKYPIDKD"
     gene            complement(2567..3559)
                     /locus_tag="VBC28_001087"
     CDS             complement(2567..3559)
                     /locus_tag="VBC28_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HEO6815080.1"
                     /translation="MKLKVFNVREEEATLAQDWANRNHVELSMSEGPLTLETVNEVEG
                     FDGIANAQIEPLDDAIYPLLKEMGIKQIAQRSAGVDMYNLELAKQHGIIISNVPSYSP
                     ESIAEFTVTIALNLIRKVELIRANVREQNFSWTLPIRGRVLGNMTVAIIGTGRIGLAT
                     AKIFKGFGCRVIGYDIYHNPMADGILEYVNSVEEAVEEADLVSLHMPPTAENTHLFNL
                     DMFKQFKKGAILMNMARGALVETKDLLEALDQGLLEGAGIDTYEFEGPYIPKNCQGQD
                     ISDKDFLRLINHPKVIYTPHAAYYTDEAVKNLVEGALNACVEVIETGTTTTKVN"
     gene            complement(3573..4631)
                     /locus_tag="VBC28_001088"
     CDS             complement(3573..4631)
                     /locus_tag="VBC28_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HEO6815081.1"
                     /translation="MSDVVEKQTTKSFIMNVLNGLALGTVIVLIPGAILGELMKALLP
                     MWSGFATLIAATAVATSMMGLVIGIMVGLNFKFNPIQSASLGLAVMFAGGAATFLKGA
                     IMLKGTGDIINMGITAALGVLLIQFLSDKTKSFTLIVIPTVTLLLVGGVGHVLLPYVK
                     MITTMIGQGIASLLGLQPVLMSILIAMIFCFLIVSPITTVGIALAISLSGIGSGAANL
                     GICAASFGLCMAGWSVNSKGTALAHVLGSPKISMANVLAKPKIMLPMISSAAILGILG
                     ALFNIQGTPASAGFGISGLIGPINALNLAKGGWSVMNMLLIIIIFVAAPIILNFIFNY
                     LFIKVLKIIDPMDYKLDI"
     gene            4824..5708
                     /locus_tag="VBC28_001089"
     CDS             4824..5708
                     /locus_tag="VBC28_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEO6815082.1"
                     /translation="MDIRQLTYFIAVAEAKNYSRAAKSLFVTQPTLSQSIKKLEAELN
                     TILFLQNGRQLALTEAGEILYEKGQLLMTNVNQMVTEIQQLNQEKKEGIRVGLTSLFA
                     IQFMKQISTFMATHSNVEVSLIQDGSRKLQELLAKGKIDIGLLSFPSTRNDITIEPLQ
                     TSTKGYKVSIVMPKSHPLATLPEIELNDLRDYKVASLNEHYMLGEMLPRKCRALGFDP
                     HIVFKHNDWEVLIHSLQDLNAVTILPSEFESISQVQDLCWVPLKDKNNFYPIGIAYRN
                     DTSFSPMIEEFLSLLKTN"
     gene            complement(5745..6563)
                     /locus_tag="VBC28_001090"
     CDS             complement(5745..6563)
                     /locus_tag="VBC28_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATPase"
                     /protein_id="HEO6815083.1"
                     /translation="MILKICRAAYSLQWGGVYQLALLDYPRIKAFELERIGAFIAYEK
                     QYKRKTEIQCDDKHLLAKIVHFLKYNSFTFPYIPKYREAAATFNEDGISLTSDFLSHT
                     CTIETAKLIFKEGKILSAVKAFNKPAEVLVKDKRNAAGDPKDYFDYVMLNWSNTNSGY
                     RLVMERLLGKAPSEQELTVGFKPGVSFHFTYQDIINHPDSIFDGYHPAKIKNQLSLAE
                     HLVACVIPKHYQEDYQSLVPNDLKHRVYYLDYCNETLYEWNQKVYDFLCHLENK"
     gene            complement(6727..7500)
                     /gene="fetB"
                     /locus_tag="VBC28_001091"
     CDS             complement(6727..7500)
                     /gene="fetB"
                     /locus_tag="VBC28_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HEO6815084.1"
                     /translation="MSGANSISLMSLLMASSLVLITLFFSYWQKLNLEKEVIISAIRA
                     VIQLLAVGFLLDYIFGYQNPIFTALLMLFMIINASYNAAKRGKGINKGFVISFIAIGS
                     GTIITLSVLIFSGILKFVPNQMIPVGGMIISNSMVAIGLCYKQLLSEFRSKQEEVETK
                     LALGADILPASIDIIRDVIKTGMVPTIDSAKTLGIVSLPGMMTGLILAGTSPIQAVKY
                     QMMVTFMLLATTSIASFVATYLAYKIFFNNRKQLVVTKD"
     gene            complement(7497..8153)
                     /locus_tag="VBC28_001092"
     CDS             complement(7497..8153)
                     /locus_tag="VBC28_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO6815085.1"
                     /translation="MSYLTFKHVDFKTDDKLVLNDINFAIDEGDFVSIVGPSGSGKST
                     VLKLASGLMSPTAGHIFFDGKDLNQLEPIESRKMISYCFQTPHLFGNTVEDNISFPYH
                     IRHEKVDYRRVDDLFQRFEMDQSYLKQDVKKLSGGEKQRIALIRQLLFEPKVLLLDEV
                     TSALDNHNKAIVEKVIKSLHDKGITILWITHDEEQSRRFANKVLKVVNGSIEAMEVIK
                     "
     gene            8370..9008
                     /locus_tag="VBC28_001093"
     CDS             8370..9008
                     /locus_tag="VBC28_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEO6815086.1"
                     /translation="MFLIDFILHIDTHIYAMANTVGNWTYLLLFLVIFVETGAVIFPF
                     LPGDSLLFAAGALAANPKMSFNIVTFLIIFFIAAFIGDSCNFLIGRTFGYRFIKHPFF
                     RRFIKEKNIRDAELYFEKKGTAAIILGRYIPIIRTFVPFVAGISQLPPKVFIKRAFIA
                     ALSWSVIATGSGFLFGNIPFVKQHFSLIILGIVFVTLIPVLISGVKSYRNTK"
     gene            complement(9086..9871)
                     /locus_tag="VBC28_001094"
     CDS             complement(9086..9871)
                     /locus_tag="VBC28_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000825435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HEO6815087.1"
                     /translation="MKRLHKLFITVIATLGMLGVMTFGLPTQPQNVTPIVHADVNSSV
                     DTSQEFQNNLKNAIGNLPFQYVNGIYELNNNQTNLNADVNVKAYVQNTIDNQQRLSTA
                     NAMLDRTIRQYQNRRDTTLPDANWKPLGWHQVATNDHYGHAVDKGHLIAYALAGNFKG
                     WDASVSNPQNVVTQTAHSNQSNQKINRGQNYYESLVRKAVDQNKRVRYRVTPLYRNDT
                     DLVPFAMHLEAKSQDGTLEFNVAIPNTQASYTMDYATGEITLN"
     gene            10132..11577
                     /locus_tag="VBC28_001095"
     CDS             10132..11577
                     /locus_tag="VBC28_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HEO6815088.1"
                     /translation="MKKTIGIIGMGVSGLAVLLALSRKKEKELKQLAIFCFDDTVHFG
                     RGIPFQEDVDSALINSPINDISFDYRHMSDFMDWLEENYYDTNLTYVSRTLYGQYLSE
                     RTKLLLDKLPVTVIKTRVDKLNYLSDEQQWQVYIQDKLFPVTFDELHLACGALPVIEP
                     YHLEGKTDYIKDPYPIKYLAQENWKGKTVAIIGTGLAAIDVIKWLLLNTQSSVKAFSI
                     TNYFPSVRIVNDLPLTWHFFTNDNLKAFLSHPSKTFTIADFNDLMSGELEHLGFKNWK
                     NTTETFLSPGIDGLKLAFKYPKQLYLLQQLASRITVWLTDLWPLMSLSDKKEYKALYG
                     KAIINLRNPMPDESAKVILKAAQKGTFSLINNVKTITKNDEGFSIEKEKQNHISVDRV
                     INATGYQLNTKNWTKANPLLKSLINQEFCQIDDQGGITILTENAQVLSPKYGPMPGLY
                     AHGALVNGVVYQNNSTIKIQQMAERAFSDNK"
     gene            complement(11634..12305)
                     /locus_tag="VBC28_001096"
     CDS             complement(11634..12305)
                     /locus_tag="VBC28_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6815089.1"
                     /translation="MLELKNIAYRYKGNDNKTLENINYSFQSGVFYTILGNSGSGKTT
                     LLSLMAGLDSPTEGQVLFNKKDIKEAGYAQHRKKNIALVFQNYNLLDYLTPLENVQLV
                     KPTADKQLLLDLGLKEDMLTRNILRLSGGQQQRVAIARALVVGTPAILLDEPTGNLDF
                     DIARDITMRLKDFAHKEKRCVIMVTHSREIAHMADTALQLIGDNLKELSKESIAQSIL
                     QQRNL"
     gene            complement(12402..12722)
                     /locus_tag="VBC28_001097"
     CDS             complement(12402..12722)
                     /locus_tag="VBC28_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEO6815090.1"
                     /translation="MIAIPAFALSVLISQFIGQKLADSLVYQASRTVRADFMKGFGNM
                     QLGADSDTDMFMQTIKTLNVQVKPEEVWLVILVGLAIVLFAIITTSLIIARYKPRGIL
                     SKLS"
     gene            complement(12731..12859)
                     /locus_tag="VBC28_001098"
     CDS             complement(12731..12859)
                     /locus_tag="VBC28_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000962057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEO6815091.1"
                     /translation="MLVVSIILVMMALLFWIGGRTHETGILLAIGKSKAIIIVSTQ"
     gene            complement(12960..13226)
                     /locus_tag="VBC28_001099"
     CDS             complement(12960..13226)
                     /locus_tag="VBC28_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6815092.1"
                     /translation="MFSKDPVRNSKDKTLVEATIVGIYKGKTVQKSNYPYEMLENTAF
                     SDTQLIKDLYGYSKGKAFYRQTTVYPKKNADIETLKSFIKKQSY"
     gene            complement(13311..13754)
                     /locus_tag="VBC28_001100"
                     /pseudo
     CDS             complement(13311..13754)
                     /locus_tag="VBC28_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048166.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(13764..14993)
                     /locus_tag="VBC28_001101"
     CDS             complement(13764..14993)
                     /locus_tag="VBC28_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001055324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6815093.1"
                     /translation="MNLWTLSLAYLKRQKMKTVTLFLVFLTIGTCLISLMSIQHSLEK
                     NILTKQGKSIYLTSKEKAYWPEQAYEALKKAKMVESVEASLSIDVGSRLKSVSPYNSS
                     KEENQVTLAGYQSTEDLRAFQTKALVLKKGSHLAADNTKQVLVPLKLAQKNHLSVGNK
                     LRLGKENVTIAGIYDANSAKSKNTFNPNIDNTLIAQATLVRKISKQKGYQTVAVRLSD
                     KRLVDTVIQNIKQWPLDFGKLDVQTAKEFYGDSYRNIETLHRLVGRIILIVSLVAMAI
                     LVVMLTFWINNRIKETGILLAIGKTKFEIIGHYLIEVLLVAGAAFTLSIIGGVFLGKT
                     FAAGLLSQVNGGVSSQIVQNSSLIIDRIDNLAVSVGVMDVFRLYAQGALICLFAVVLS
                     SYSILKLQPKQILSRMS"
     gene            complement(15117..16178)
                     /locus_tag="VBC28_001102"
     CDS             complement(15117..16178)
                     /locus_tag="VBC28_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEO6815094.1"
                     /translation="MNKITTLSTIALTLMLCVGCSANKDNQKTKTEDPKQENVTANPK
                     EDKATAAKRMKLTKELEKYNKGTESFISNKQYQKYIKTPIKEYGQKVEVKGKKMNVYT
                     VGEGKVPIVFIPGQGTVTAKHQYHNLISNLSKTHKVVVVEPFGSGLSDVIDQPRNLAN
                     ITSDIHEALQKVGITGKYVIASHSIGGVYALKYISTYPKEVLGLIGLDTSTPGMEGGK
                     QVDFAAPVLKELPKIPKVSDDINAQFFAIGHKILNNSNMKEEAKNSSNMINESANYKI
                     PKGIPAMYLLARESDLGLQLRSLDIPIKGSWQEQHKELSEDPKEVSIHTLDGTHQIYD
                     TNLKEVIDYTNQFMKTFEK"
     gene            complement(16213..17232)
                     /locus_tag="VBC28_001103"
     CDS             complement(16213..17232)
                     /locus_tag="VBC28_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEO6815095.1"
                     /translation="MNKITKLSTVALALMLCVGCSANKDNQKTKTEQPKQEKSIAKQK
                     QEREAEKAMIAKGAIDLTKYKAKPIEDYGQKIDVGDGKKMNIYTMGQGKIPLVVFPGQ
                     AEISPRYAYKNLLDRLGKKYKIYTVEPLGYGLSDMTDKPRTIENITKEIHTALEKVGV
                     KNFYLMAHSLGGIYGLNYAKTYSNDVKGFIGMDTSTPNMEGDGTTGESSASTLKWAES
                     IPEVDKKTNEQYLSIGAKKSGNKTERDKDRRVSDNLHKMEKVKFPKGMPAKYFLANES
                     AEDIKMRKKIFPKLKSWEQQHIDLSEDPKDVSVETLDASHLIYHTQYKHMAKAIDEWI
                     QSHEK"
     gene            complement(17267..18298)
                     /locus_tag="VBC28_001104"
     CDS             complement(17267..18298)
                     /locus_tag="VBC28_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEO6815096.1"
                     /translation="MKNITKLSTVALSLLLCTACAASNTSTSKTQSHHPKQTKLTDKQ
                     KEEPKNKEAADQEMHPQGAVDLTKYKAKPVKDYGKKIDVGDGKKMNIYETGQGKIPIV
                     FIPGQAEISPRYAYKNLIERLSKKYKIYTVEPLGYGLSDIPTKPRTLENITKEIHTGL
                     NKIGVKNFYLAAHSLGGMYSLNYAKNYPEEVRGFIGMDTSTPWMEGEQKTKYDPESAK
                     WAMKLPDVDDKTNEQYLSIAKKINGNKSQRDEDERLGDNLGKLKNVKFPKGMPTKYFL
                     ADQSVEGIKLRQADFKGLKNWDQQHIDLSEDPKDASVEILKGSHNIFQTQYEHMAKAI
                     DGFIQSHQK"
     gene            complement(18437..19255)
                     /locus_tag="VBC28_001105"
     CDS             complement(18437..19255)
                     /locus_tag="VBC28_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HEO6815097.1"
                     /translation="MGDYYGKKYFGEAAKKDVEHMAKKIINVYKTRLKNNTWLSENTK
                     AMAIKKLDNMRLMIGYPEDYPDLYRQYQFDSKASFFENNDNYRKLSNKKTFEEFNQSN
                     QREHWQMSANAVNAYNDPNTNSIVFPAAIFQSPLYDKTKTVSQNYGAIGAIIGHEISH
                     SFDINGMKYDEKGNLHDWWTKEDLKHYKKKTQAMIDQWDGLKADGGKVDGKLTLAENI
                     ADNGGVMASLEALKTEKIQTIKNFLNHGQVFGVKKQPKNKVSPQFSQMFMHHMN"
     gene            complement(19276..20361)
                     /locus_tag="VBC28_001106"
     CDS             complement(19276..20361)
                     /locus_tag="VBC28_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001873818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M13"
                     /protein_id="HEO6815098.1"
                     /translation="MQRKSYLKSMSVLTLTACLISGYVVKDIAMLHAVSASEKKANNV
                     SPRENLYRAVNDNWLANTKLKQGQTSVNSFSEIEDKLKQLLVSDMAKMASGKIETTND
                     EQKKMVAYYKQGMDFKTRDKNGLKPLKPVLQKLEAVSSMKDFQSLAHDFVMSGFVLPF
                     GLTVETNARDNSQKQLVLRQAPALLESPDQYKKGNKEGEAKLSAYRTSAMALLKQAGK
                     SNIEDRKLVKQAIAFDRLLSEKTQVDQSKITAESETAAGRYNPESMETVHNYAKEFDF
                     KELIEKLVGPTNKAVNVEDKTYFKQVNDVINSKQLANMKAWMMISMLVDQSDFLGEQN
                     RQAASAFKNVASGLTQIESKEKMLTPN"
BASE COUNT         6423 a         3867 c         3159 g         7036 t
ORIGIN      
        1 tccatttccc tttgagtaaa ggcaatatag tctaacccat attcttggac aatcttttgg
       61 gttatctttt caagtaattc aatatcaagg taaggtctgg ataagcccaa ctcatctttt
      121 aaatcttcac tttcgccagc taaataaatc ggctctaagc caaagcaaac atctaataat
      181 cctaatagtg gcgataattg ctcacgtgcc tcttgaaaag atgaccataa actctcacga
      241 aaattgagat caatcgatac ttttacacct ccttctttag cttttgtcat aaggaaccga
      301 gtcacctcat ataagtcctt ggttagtgct ggagtaattc cagaaacatg aaaccaatcc
      361 actcccttaa aaatagaagt aaaatcataa tcactcaagg tactttccca aaaagatgaa
      421 tagttacgat cgtagataac cttatttgtt cttaccgaaa atccttgttc atagtaataa
      481 attcctagtc gtccttcttt tttaattata gcagctggac taatttgctg tgacaatata
      541 aattgacttg ccatcttgcc caaatcatta tcaggcaaag ctgagaccaa tcctacatgg
      601 taacctagtt gagcaaggct tgctaagaca ttaagttctg aaccaccgaa ttggcatttt
      661 aggtgattag cctgcatcag tgtgtgatat tgaggtggag ataatcgcaa aagaacctca
      721 cctaatgaaa taatttttgc cataagaatc ctccaatggt agctcttatt taacaaaata
      781 gctgctctat tgaagagcag ctaaactttt taatctttat caataggata ttttgttaca
      841 tcaacataat ccatatctgt caaaggaaca ttacgagcaa tatctttagc acgttcctta
      901 gtagttgctg ctttcataac ttgaatcttc tcttgaatac gaaccatttc ttctttatct
      961 agtttgtaaa atttcattaa aagaagagct atagacaaag ctataaatgg aatggccaca
     1021 aggatactaa tagctgccat ttttaaatca gggggcagtg gtgtttcgat agttggaaat
     1081 gattttgaaa atccaattcc tgctaacaca aaacctacta ccataggagc aaatgaagaa
     1141 gcaatcgaat ctgttaagga gaagatagtt ccaatcattc ctgatacata acgaccggat
     1201 tcagaagttt catagtcaga aatatctgct cccatagtca atactaaact agcaggtgct
     1261 tgtgaagcgt atctcgcaat aatattagta acgataaata aaatagtata aagatttagg
     1321 cttgttaaac taaggtcacc aggcttacca acgtaaagaa ctgcaccaaa agcaagtaaa
     1381 ccaatcatac cgatctgaat tgctctgaca tatgaaaaac gaagaccttt tttacgagca
     1441 attgttgaga acaaaatatt aataataaca ccaggaacaa tgaaaagaag tgagaattgt
     1501 cctgataaag cataattacc aaataaaata ccaaacaata gcaccataac aacggaatcg
     1561 ccaaagaatt ggatggcaaa ttttaccaaa gctgcagcaa tggataatat ctgaagcggt
     1621 ttatttcctt tcaacacctt ccagtaatct tttagagcag tcttttgggt attctctcct
     1681 aaaccaaaga attcttttct gtcttttgcc caaatcccaa taattgcaac gattgctaaa
     1741 atagctgaaa taagaattgt tccaaaaata aggacattga agaattgagg tgtaaaatta
     1801 ccaaattttg gtactaagaa aactgaaacg acaaactgac cgcctgtcat taatgatgtt
     1861 gtcataactg catcaacgat attaaaaata ggacgttgct tagggtcatt tgtcagtgct
     1921 gtttgccctg ctttagttat tgtctgttgc attgagtatc caattttatg gataattaga
     1981 acaaggataa ataatgggaa acgaatattt tcatcgacac ctctcaatgc aagaagaaaa
     2041 atcaatgaca aagccgtaat aacgttccca ataatcaaaa taggacgata cttaccaaat
     2101 tttgtatcag ttttatcaat cataatacca atagctggat caataaaacc atcaaaaata
     2161 cggatataac ccataatttg actgacaaaa atggctgccg agccaagaac gccagttgag
     2221 aaataggtaa caaacataaa cgtaaataag tatatatttg tagaagcatt gttcatggca
     2281 aacataacta actgccatac tttcgctcgg ttataagata caacttcttc acgttgttcc
     2341 atatccattg ttttctccat gagagaaccc tccttgtatt ttgtaaacgc ttttatgatt
     2401 aaataatact attcatctga tgttttttta atatttttat aaaaaatatc tttttttatt
     2461 ataaaaaaga ctttccagct ataaatacat aagatgtttt tttgctttaa aggtcatgat
     2521 catcatatgt tttgttaccc cacaaaaaaa gatgcctaag catcttttag ttgactttcg
     2581 tagtagttgt tcccgtttca atcacttcta cacaagcatt taaggcaccc tctactagat
     2641 ttttgaccgc ttcatctgta taataagccg catgaggagt gtatattact ttcggatggt
     2701 taatcagtct taggaaatcc ttgtctgaaa tgtcttgacc ttgacaattt ttaggtatat
     2761 atggcccctc aaattcataa gtatcaatac cagctccctc caaaagccct tgatctaaag
     2821 cctcaagaag atctttggtt tccaccaatg ctccacgtgc catattcatt aaaatagcac
     2881 cttttttaaa ctgtttaaac atatctaaat taaatagatg cgtattttca gcagttggtg
     2941 gcatatgtaa ggacacaaga tcagcttctt caacagcttc ttctaccgaa ttgacatact
     3001 caaggatacc atctgccatt ggattatggt aaatatcata accaataaca cgacatccga
     3061 accctttaaa aattttagca gtcgctagac caatgcgacc agttcctatt atagccacag
     3121 tcatgttacc taggactcgt cctcgaattg gcaaggtcca tgaaaaattc tgttcacgaa
     3181 cattagctct aataagctca acttttcgaa taaggttaag agcgattgtc actgtaaatt
     3241 ctgcaataga ctccggtgag taacttggca cattgctaat gataatacca tgttgcttag
     3301 ctaactcaag attatacata tcaacaccag cactgcgctg agcgatttgc ttaattccca
     3361 tttctttgag aagaggataa atagcatcat ctaaaggctc aatctgtgca ttagcaatcc
     3421 catcaaaacc ttctacctca ttaacagttt ccaatgtcaa aggtccttca ctcatagaaa
     3481 gttccacatg atttcggttt gcccaatctt gtgctaatgt tgcttcttct tctctaacat
     3541 taaatacttt gagtttcatc actaatatct ccttaaatat ctaatttata gtccattgga
     3601 tcaataattt taagtacttt tataaagagg taattaaaaa taaaattcaa tataattggt
     3661 gcagcaacaa atattataat tatcaataac atattcataa ctgaccatcc accttttgct
     3721 aaattaagag cattaatcgg tccaatcaaa ccactgatac caaaacctgc actagctggt
     3781 gttccttgaa tattaaagag agctccgaga attcctagta ttgctgctga agatatcata
     3841 ggtagcataa tcttcggttt tgctaataca ttagccatcg aaatcttagg agaccctaaa
     3901 acatgcgcta aggctgtccc cttagaattg actgaccacc cagccataca aagcccaaaa
     3961 ctagcagcac agatacctaa attagcagca ccagaaccaa ttccggacaa actaattgcc
     4021 aaagcaatcc caactgttgt gattggagat acgatcaaga aacagaaaat catcgcaatt
     4081 aaaattgaca tcaaaacagg ttgtaaacca agtaatgaag caatcccttg tccaatcatg
     4141 gtggtaatca ttttgacata aggtaataac acatgaccta ctcctccaac tagtaagagt
     4201 gttactgttg gaatgacaat caaagtgaat gatttcgtct tgtcagataa aaattggatc
     4261 aaaaggacac ctaaggcagc agtaattccc atgttaatga tatcgcctgt tccttttagc
     4321 ataatagcgc ctttaaggaa ggttgcagct ccacctgcaa acataaccgc taatcctagt
     4381 gatgctgatt gaatgggatt aaatttaaaa ttgagaccaa ccataatacc aataacaagc
     4441 cccatcatac ttgttgcaac tgctgttgca gcgattaaag ttgcaaagcc gctccacatt
     4501 ggcagtaaag ctttcattaa ttctccaagg attgctcctg gtatcaagac aataacagtc
     4561 cctaaagcta aaccatttaa tacgttcatt ataaacgatt tagttgtttg tttttcgaca
     4621 acatccgaca taagatgcct cctactaata atgattgttt gtgatttcac taactatatt
     4681 aactatatca tagctatttg caaaaggaaa attggaaaaa attatataag tatagctttt
     4741 actaatatta ttgtggaaat aatcataaag gtcagattga tagacttgtg ctatactatt
     4801 actaagaaaa aaggacagat attatggata ttagacaact tacttacttt attgctgttg
     4861 cagaagcaaa aaattattca cgcgctgcaa aaagtctttt tgtgacccaa cctacacttt
     4921 cacaatccat taaaaaatta gaagcagaat taaatactat tttattctta caaaatggtc
     4981 gtcaactcgc tttaactgaa gcaggagaaa ttctctatga gaaaggacaa ttgttgatga
     5041 ctaatgttaa tcaaatggtt acggagatcc agcaattaaa tcaagaaaaa aaggaaggta
     5101 tccgtgtagg gcttacttct ctttttgcta ttcaatttat gaaacagatt tcaactttta
     5161 tggctacaca ttcaaatgtt gaagtatctt tgattcaaga tggttcccgt aaattacaag
     5221 aattgttagc aaaaggaaag atcgatattg gcttattgtc ctttccaagt acccgcaatg
     5281 atatcactat cgaaccactg caaacctcaa ctaaaggata taaagtaagc attgtcatgc
     5341 caaaatcgca tcctcttgca acacttcctg aaattgaact taatgattta agagattata
     5401 aagttgcctc actcaacgaa cattatatgc taggagagat gttgcctcgt aaatgtcgtg
     5461 ctttaggatt tgacccccat atcgtcttta aacacaatga ctgggaagtt cttattcata
     5521 gtttacaaga cctcaatgca gtcactattt taccaagtga atttgaaagc attagccaag
     5581 ttcaagattt atgctgggtc ccacttaagg ataaaaataa tttttatccg attggtattg
     5641 cttaccgaaa tgatacttct tttagtccta tgattgaaga atttttaagt cttttaaaga
     5701 ccaattaata gcagttaaac caaaaagttt taaacttttt ggttttattt attttccaaa
     5761 tgacaaagaa aatcataaac tttttgattc cactcataaa gtgtttcgtt acagtaatct
     5821 aaataataaa ccctgtgttt caagtcattg ggcacaaggc tttgataatc ttcttgataa
     5881 tgttttggga taacacatgc aactaaatgt tctgctaaag aaagctgatt tttaatttta
     5941 gcaggatgat aaccatcaaa aatagaatca ggatgattga tgatatcttg ataagtaaaa
     6001 tgaaaactga cccctggctt aaaacctact gttaactcct gttcagatgg tgctttgcct
     6061 aacaatcttt ccattactaa acgataacca gaattggtat ttgaccagtt caacatcaca
     6121 tagtcaaagt aatctttagg gtctccagca gcattcctct tatcttttac cagtacttca
     6181 gcaggcttat taaaggcttt aactgctgat aagattttac cttctttaaa aattagtttt
     6241 gcagtttcaa tcgtacatgt atggcttaaa aaatcagaag ttaaactaat accatcctca
     6301 ttaaaagtag ctgccgcttc tctatatttg ggaatatagg gaaaagtaaa actattgtat
     6361 tttaaaaaat gaacaatttt tgcgaggaga tgtttatcgt cacattgtat ctcagttttt
     6421 cttttatatt gtttctcgta agctatgaaa gctcctatcc tttccaattc aaacgcctta
     6481 attcgaggat aatccagcaa agctaattgg taaacacctc cccattgtaa actatatgct
     6541 gcacgacaaa tttttaaaat catttcttcc tccttttgat aatccctaca acaatttcta
     6601 ttaaatagta gcatttaaac gtaatcgact caataacttg gtcaattttc gatcaaaaat
     6661 aagattaaga taccaaaaaa gcctggacta tacttaccag actaacatac tgtttattaa
     6721 aatgaatcaa tcttttgtta ctactaattg cttacgatta ttaaagaaaa ttttataagc
     6781 caaataagta gctacaaaag aagctatcga agtcgttgct aatagcataa acgtcaccat
     6841 catttggtat ttaaccgctt ggataggtga agttccagca agaattaacc ctgtcatcat
     6901 ccctggcaaa gatacaatcc caagtgtttt agctgaatca attgtaggta ccataccagt
     6961 tttgataaca tcacgaataa tatcaatcga agctggcaaa atatctgctc ctaaagctaa
     7021 ttttgtctcc acttcctctt gtttacttct aaattcggac aataattgtt tatagcacaa
     7081 accgattgct accatagaat tactaataat cataccacct acaggaatca tttgattcgg
     7141 aacaaatttt aaaattcctg aaaaaatcaa gacagataaa gtgattatag tccctgaccc
     7201 aatagctata aatgaaataa caaacccttt attaatccct ttaccacgct tagctgcgtt
     7261 ataagaagca ttaataatca tgaatagcat taaaagagct gtgaaaatag gattttgata
     7321 accaaatata tagtctagca aaaaacccac tgctaataat tgaatcactg ctcggatagc
     7381 gctaataatc acttcttttt ctaggtttaa cttttgccag taagaaaaaa ataaagtaat
     7441 caaaactaat gacgatgcca ttagtaatga cattaaagaa atagaatttg caccgctcat
     7501 tttataacct ccattgcttc aatactacca ttgacaactt ttaagacttt attggcaaat
     7561 cgacgactct gttcctcatc atgcgtaatc cacaaaatcg ttattccttt gtcatgtaat
     7621 gatttaataa ccttttcaac aatagcttta ttatgattgt ctaaagccga agtaacttca
     7681 tctaagagta aaactttagg ttcaaacaat aattgcctaa tcagagcaat acgttgcttt
     7741 tctcctcctg atagtttttt gacatcctgt ttcaggtaag actggtccat ttcaaaacgt
     7801 tgaaataaat catcaacgcg tctataatct actttttcgt gtcgaatgtg gtaaggaaaa
     7861 ctaatattgt cttccactgt attaccaaat aaatgtggcg tttgaaaaca gtaagaaatc
     7921 atttttcgac tttcaatcgg ttctaattgg ttcaaatctt ttccatcaaa gaaaatatga
     7981 cctgcagtag gactcattaa accactggct aattttaata cagtactctt accacttcca
     8041 gatggcccta caatagaaac aaaatcacct tcatcaattg caaagttgat atcatttaaa
     8101 accaacttat catccgtttt aaaatcaacg tgtttaaagg ttaaatagga cattgttaag
     8161 ctcctttatt tcttatttat aatcattata attaagtcta acataaaaat caaaaaaaag
     8221 ctctttagaa taattctaaa ttaataagta aacttatttt acctcttatt aaaaattttc
     8281 tgataaaata ggctttgtaa gaggccttat aaacaagata gttatcatcc aagtctctgc
     8341 actttagttg atcaataaag gagactccta tgtttttaat cgattttatt ttacatattg
     8401 atactcatat ctatgctatg gctaacactg ttggtaattg gacctatcta ttgctcttcc
     8461 tcgttatttt cgttgaaact ggggctgtca tctttccatt tttaccaggt gatagtttat
     8521 tatttgctgc tggagccctt gctgcaaatc caaaaatgtc cttcaatatt gtaacttttc
     8581 ttattatttt ctttattgca gcatttattg gtgatagttg caacttcttg attggtcgta
     8641 catttggtta ccgttttatc aaacatcctt tctttcgtcg tttcattaaa gaaaaaaata
     8701 tccgagatgc tgagctttat tttgagaaaa aaggtactgc tgccatcatt ttagggcgct
     8761 atatccctat tatcagaacc tttgttcctt ttgtagcagg tattagtcag ctccctccaa
     8821 aagtatttat caaacgcgcc ttcattgcag cattatcttg gtcagttatt gcaacaggtt
     8881 ctggttttct ctttggtaat attccttttg tgaagcagca cttctcgctt attatcttgg
     8941 gaattgtatt tgttacctta attcctgtcc ttatttctgg tgtcaaatca tatcggaaca
     9001 ctaagtaatg tatccaaaaa tttctattta acactattaa tcactaagaa tgacaacaaa
     9061 aaaagagcaa ttagttgctc ttttattaat ttagtgttat ttctcctgtt gcataatcca
     9121 tagtgtatga tgcttgtgtg tttggaatag caacattaaa ttctaatgtg ccatcttgtg
     9181 atttagcttc taggtgcatt gcaaatggaa ctaaatcagt atcattacgg tacaatggag
     9241 ttacacggta acgaacacgt ttgttttggt caaccgcctt acgaactaag ctttcataat
     9301 aattttgtcc acgattgatt ttttgatttg attggttgga atgagctgtt tgtgtgacaa
     9361 cattttgagg atttgacacg gaagcatccc aacctttgaa atttccagct aaagcatagg
     9421 caattaaatg ccccttgtcg actgcatgtc cataatggtc attagtagct acttgatgcc
     9481 aacctaatgg tttccaattt gcatcgggaa gagtggtatc tctgcgattt tgatattgac
     9541 gaatggttct atcaagcatt gcattagcag ttgatagtct ttgttgattg tcaattgtat
     9601 tttgaacata cgctttaaca ttgacatcag catttaaatt tgtctgatta ttatttaatt
     9661 cataaatacc attaacatat tgaaatggta ggttaccaat agcatttttt aaattatttt
     9721 gaaattcctg gctcgtatca acagatgaat tgacatcagc atgtactatc ggcgttacgt
     9781 tttgcggctg cgttggaaga ccaaaggtca ttacccccaa catacctaat gtagcaatta
     9841 cggttataaa cagtttatgt aatcttttca taagctttac ctctttctaa ttacattaaa
     9901 caagcgtcta ccatattgat atccgtttta gctaattgct ctccttttgt cacaaccata
     9961 tcatatgcct attatttaag catgtcaatc atttatttaa tcttatatta atttaatatt
    10021 actttaaatt taatttactc attaaattag aaaatctgca aataatagct ttgttcatcc
    10081 cctaacttaa atcaaaattt atgctatgat agtggtaagg aggcttgttc tgtgaaaaaa
    10141 acaattggta ttataggtat gggagttagt ggtttagctg ttctactagc actttctcgt
    10201 aaaaaagaga aagagttaaa acagttagct attttttgtt ttgatgatac tgtccacttt
    10261 ggacgaggaa ttccttttca agaagacgtt gactcagctt taatcaattc cccaatcaac
    10321 gatatttcat ttgattatcg ccatatgtct gacttcatgg attggttaga agaaaattac
    10381 tatgacacaa atctaaccta tgtatcaagg acactttacg gacaatacct ttcagaaaga
    10441 actaagttac ttttagataa actccctgtc acggttatta aaacaagagt ggataaacta
    10501 aactatttat cagatgagca acaatggcaa gtctatattc aagataagct ttttcctgtc
    10561 acatttgatg aattacactt agcttgtggt gctctgcctg ttatagaacc ttatcatttg
    10621 gaagggaaga ccgattatat caaagaccct tatcctatca aatacttggc tcaggaaaat
    10681 tggaagggaa aaacagttgc tatcatcggg actggtctag ctgcaattga tgttataaaa
    10741 tggttgctac tcaatacaca atcttctgtt aaagcatttt caataaccaa ctacttccct
    10801 agcgtccgta ttgttaatga tttaccactt acttggcact tttttaccaa tgataacctt
    10861 aaagcatttc tatcacatcc ttcaaaaacc ttcactattg ccgattttaa tgatttgatg
    10921 tctggcgaat tggagcatct tggctttaaa aactggaaaa atacaacaga aactttttta
    10981 tccccaggaa ttgatggtct caagttagcc tttaaatacc ctaagcaact ctatctatta
    11041 caacaattag cttcacgaat cactgtctgg ttaactgacc tttggccact catgtcatta
    11101 tcagataaga aagagtataa agcactctat ggaaaagcga ttattaactt gcgaaatcct
    11161 atgcctgatg aatctgctaa agtaattttg aaagctgcac aaaaagggac tttttctctg
    11221 atcaataacg tcaagacaat tacaaaaaat gacgaaggat tttcaataga aaaagaaaaa
    11281 caaaatcata tatctgtcga tcgcgtcatt aacgctactg gttaccagtt aaacactaaa
    11341 aactggacaa aagccaatcc acttctaaaa tcgctcatca atcaagaatt ttgtcaaatt
    11401 gacgatcaag gagggattac tattttaact gaaaatgcac aagttttgtc tccaaaatat
    11461 ggtccaatgc caggtttata tgcgcatggt gcactggtta atggtgtcgt ttatcagaat
    11521 aactctacaa ttaaaattca acaaatggca gagcgagcct tttctgataa taaataacca
    11581 tttctaaaaa atacctcctc ttttattatt taagaaaggg aggtattttt ttattataaa
    11641 ttcctttgtt gtaatataga ctgtgctata ctttcttttg ataattcttt taagttatct
    11701 cctatcaatt gtaaagcagt atcagccata tgagcgatct ctctactatg tgtcaccata
    11761 ataacacatc gtttttcctt atgcgcaaaa tctttaagac gcatcgtaat atcacgcgca
    11821 atgtcaaaat ctaaattccc cgtaggttca tcaagtaaaa tagctggtgt tcctacaacc
    11881 aaagctctcg caatagctac acgttgttgt tgccctcctg ataatctcaa gatatttcgc
    11941 gttaacatat cttcttttaa acctaaatcc aacaataatt gtttatctgc agtaggtttt
    12001 accaactgaa cattttccaa tggggttaga tagtctaaaa ggttataatt ttgaaagacc
    12061 aaagctatat tctttttgcg atgctgagca taacctgctt ctttaatatc ttttttgtta
    12121 aataatactt gtccctcagt aggcgaatca agacctgcca tcaaggatag aagggttgtt
    12181 ttccctgaac cagaattccc taatatagta taaaataccc ctgactgaaa actataattg
    12241 atattttcta atgttttatt gtcgttcccc ttataacgat aagcaatatt ttttaattcg
    12301 agcatagatt tctctttctt gagctatgta ttcaatagca gagcatattc attttaaccg
    12361 ttaatataga tgtcactatt ggtcggtata cttatggcac tttaggacag ttttgataag
    12421 attccccttg gtttgtagcg agcaataatt aaactagtag taataatagc aaataagacg
    12481 attgctaaac caactagaat tactaaccag acttcctcag gcttcacttg tacatttaac
    12541 gtcttaatcg tttgcataaa catatctgta tcactatcag ccccaagctg catattccca
    12601 aaccctttca taaagtctgc tctgacagtt ctagaggcct gatacactaa ggagtctgct
    12661 aatttttgac caataaattg actgataagt actgataaag caaaggctgg aatagcaatc
    12721 attagtactt ctactgcgta ctgacaatga taattgcttt agacttacca atagctaata
    12781 aaatacctgt ctcatgggtt ctaccaccaa tccagaatag cagagccatc atcactagga
    12841 taatagatac tactaacatg ccgccttgta gagaagtaat taattgattc aagacatcta
    12901 tactttttgc ataacttgtt agcaaggttg ctgtaattgt cagttggtat ttattccaat
    12961 caataggatt gttttttgat aaaactttta agagtttcta tatcagcatt ctttttagga
    13021 tatacagttg tttggcgata aaaggcctta cctttagaat agccgtataa atccttaatc
    13081 aattgcgtat ctgaaaaggc tgtattttct agcatctcat aagggtaatt tgatttttgt
    13141 accgttttac ctttatatat gccaacaata gttgcttcaa ctaatgtttt atccttactg
    13201 tttctaacgg ggtcttttga aaacattaac ttatcgccaa ctttaagatg atttgtcttc
    13261 gcaaaagcct gatgaacaat tataactcgc tgatctttcg gcgtgatatg tcaccctccc
    13321 ttaagagaaa tcatccccgc tgtgtattta ggatctgtat cggagtaact attaccatga
    13381 acttgtaaaa cttgttcttt atattgttct ttatcatttg gtcatgtaac cattttttcc
    13441 gaggttagtc cagcataagc catcaaatta ttattaacgc gcttaacatt tggattactt
    13501 gctatgttaa ctattgcttt ttcagggatt tctccagatc cgtaaccatc accaacaagg
    13561 tcagtactat ccgatgaaat atgcaaaatt gatccagttt gccttgaaaa tgtcttctta
    13621 gcagtaagac cagcattttt taccgcaata ccgctaatta atgtagaagc aactagccat
    13681 aaacatacga ataaagtgat actcttacgc tttttacggc taacatataa aaaggaacgt
    13741 ttaaataaat tcatattaac ctcctatgac attcgtgaca atatttgctt tggttgtagt
    13801 ttaagaatag aataactaga taatacaact gcaaataagc aaattaaagc accctgagca
    13861 tagagtctaa agacatccat tactccaaca gaaacagcga ggttatcaat tctatcaata
    13921 attagactac tattttgtac aatttgtgaa ctcacaccac cattaacttg tgataaaagg
    13981 ccagctgcaa atgtttttcc aagaaagaca ccaccaataa tagatagtgt gaaggcagca
    14041 cctgctacta acagtacttc tatcagataa tgacctatga tttcaaactt agtttttccg
    14101 atagcgagta aaattcccgt ctctttaatg cggttattaa tccaaaatgt tagcatgaca
    14161 actaatattg ccatcgccac gagactaaca ataagaatga ttcgtcccac tagacgatgc
    14221 aatgtctcga tattacgata agaatctcca taaaactctt tagctgtttg aacatctaat
    14281 ttcccgaaat caagcggcca ttgtttgatg ttttggataa ctgtatccac taaacgttta
    14341 tcacttaagc gcactgcaac cgtctgataa cctttttgtt tactaatctt cctaaccaat
    14401 gttgcctgag caatcaatgt attatcaata ttgggattaa aagtattttt agacttagca
    14461 ctattcgcat cataaattcc tgcaatagta acattttctt tgcctaatct caatttgtta
    14521 cccacactta aatgattctt ttgtgctaac ttcaatggaa ctagtacttg ttttgtatta
    14581 tcggccgcta aatggcttcc tttctttaag accaatgctt ttgtctgaaa agctcgtaaa
    14641 tcttctgtac tttgataacc tgctaatgta acttgattct cttctttaga agagttatat
    14701 ggtgatacag attttaaacg gctaccaaca tcaatgctta aacttgcctc tactgactca
    14761 accatttttg cttttttcaa agcttcgtat gcttgttcag gccaataagc cttttctttg
    14821 gaggttaaat aaatgctttt cccttgtttt gttaggatat ttttttccaa agaatgctga
    14881 atactcatca gactgattaa acatgtgcct atagttagaa aaactaagaa caaggttact
    14941 gttttcattt tttgacgttt caaataagct aaacttaatg tccataggtt cactattttc
    15001 cctccttttt ctatcttaat aatctctaga ataacgatag aagtcgttag atattctcta
    15061 tcgacttcta cgttatctat caattagtga ttttatagca ctgtttctaa aaacaattat
    15121 ttctcaaatg ttttcataaa ttggttagta taatcaatta cttctttaag gttagtatca
    15181 taaatttggt gagttccgtc tagtgtatga atagagactt cttttggatc ttctgataac
    15241 tccttatgtt gctcttgcca actgccttta attggtatat ctaaactacg taattgaagc
    15301 cctaagtcac tttcacgtgc taataaatac atagcaggaa ttccctttgg aatcttatag
    15361 ttggcacttt cgttaatcat attagaagaa ttcttcgctt cttctttcat attagaatta
    15421 ttaagtatct tatgtccaat agcaaaaaat tgtgcattaa tatcgtcgga tactttagga
    15481 attttaggta attcttttaa cactggagca gcgaaatcaa cttgcttacc accttccata
    15541 cccggcgttg atgtatctaa acctattaat cctagcactt cctttggata agtactgatg
    15601 tatttaaggg cataaacacc accaattgaa tgagaggcaa ttacatattt tccggtaata
    15661 ccaacttttt gtaaagcttc gtggatatct gaggtaatat tagctagatt tcgtggttgg
    15721 tcaatgacat cacttaaacc tgaaccaaat ggctcaacta ccacaacctt atgagtttta
    15781 cttaaattac taatcaaatt atggtactga tgtttggcag tcaccgtccc ttgaccagga
    15841 ataaagacaa ttggtacttt accttctcca acagtataga cattcatctt cttgcctttg
    15901 acttcaacct tttgaccata ttctttgata ggtgttttga tatatttttg atattgtttg
    15961 ttactaataa agctctctgt gcctttatta tatttttcaa gttctttcgt taacttcatt
    16021 cgcttggctg ctgttgcctt gtcttccttc ggatttgcag ttacgttttc ttgcttagga
    16081 tcctcagttt tagttttttg attatcttta ttggcagaac atccaacgca aagcattaaa
    16141 gtcagggcga tggttgataa tgtcgttatt ttattcatct tttctcctta gtaaaataat
    16201 tggggggaat aattattttt catgcgattg aatccactcg tcaatagcct tcgccatatg
    16261 tttatattgc gtatgataaa tcaagtggct agcatctagt gtctcaacag aaacgtcttt
    16321 tggatcttca gataagtcaa tatgttgttg ttcccagctt ttaagcttag ggaatatttt
    16381 tttacgcatt tttatatctt ctgctgactc attagctaag aaatacttag ctggcatacc
    16441 tttagggaac ttaacttttt ccattttatg aagattgtca ctaacacgcc tatctttatc
    16501 gcgctctgtt ttattacctg attttttagc accgattgat aagtattgtt cattggtttt
    16561 cttatcaact tctggaatgg attctgccca ttttaaggtt gaagctgaag attctccagt
    16621 tgtgccatca ccttccatat ttggtgtgct tgtatccatg ccaatgaaac ctttgacatc
    16681 gttagaatat gtcttagcat aatttagacc gtatatgcca cctaacgagt gagccataag
    16741 atagaaattt ttgacaccga ctttttcaag agctgtatga atctctttgg taatattttc
    16801 aatcgtacgt ggcttatctg tcatatcact aagcccataa ccgagcggtt caaccgtata
    16861 aattttatat tttttcccta atctatcaag caagtttttg taagcataac gtggtgaaat
    16921 ttcagcttga ccagggaaga ctactagcgg aattttccct tgacccattg tatatatgtt
    16981 cattttttta ccgtcaccga catcaatttt ttgtccgtaa tcttcaattg gtttcgcttt
    17041 atatttagtt aggtcaatag cacctttagc aatcatagct ttttctgctt ctctctcttg
    17101 tttttgctta gcgattgact tttcttgctt aggctgctca gttttagttt tttgattatc
    17161 tttattggca gaacatccaa cgcaaagcat caaagcgagg gcaacggttg ataatttcgt
    17221 tattttattc attttttctc cttgacttag gtatttcatt caacgattac ttttgatggg
    17281 attggataaa tccatcaata gctttagcca tatgttcata ttgtgtttgg aaaatattgt
    17341 gactaccttt aagaatttca acggaagcat cttttggatc ttcagataaa tcaatatgct
    17401 gctgatccca atttttcaaa cctttaaaat ctgcttgacg tagcttgatt ccctccacag
    17461 attgatcagc aagaaaatac ttagttggca tccctttagg gaattttaca ttcttcaatt
    17521 taccaagatt atcacctaag cgctcatctt catctcgttg agacttattg ccgttaattt
    17581 ttttcgcaat tgacagatac tgttcgttag tcttatcatc aacatctggt aatttcattg
    17641 cccatttagc actttcaggg tcatactttg ttttttgttc cccttccatc caaggtgtac
    17701 tggtatccat accaataaat cctctgactt cttctggata atttttagca taatttaaag
    17761 aatacatacc gccaagtgag tgagctgcaa ggtagaaatt tttaactcca attttattta
    17821 ggcctgtgtg aatttctttt gtaatgtttt ctagcgtacg tggtttagta ggaatatcac
    17881 tgagaccata gcctaatggt tctactgtat aaattttata ttttttactt aaccgctcaa
    17941 ttaaattctt ataagcatag cgtggcgaaa tctcagcttg accaggaata aaaacaattg
    18001 gaatttttcc ctgaccagtt tcataaatgt tcattttctt gccatcacca acatcgattt
    18061 ttttaccata atctttgacc ggttttgcct tatattttgt caaatcaaca gcgccttggg
    18121 gatgcatctc ttgatcagca gcttctttgt ttttgggttc ttctttttgc ttatctgtga
    18181 gtttagtttg tttaggatga tgagactgtg ttttagatgt agacgtgttt gatgcagcac
    18241 acgccgtaca aagtagtagg cttaaagcaa cagttgatag ctttgttata ttcttcatgc
    18301 attctcctta ttgtctggaa aacatagctt taccaaagtg tcaaacgttt ttctggtttt
    18361 agatacattg aatcgccttt tttaacacca aaggcatcat aaaattcttg gaaattacgt
    18421 actgggatgt tagctctcaa ttcatatggt gcatgaacat ctgactgaat tgaggactta
    18481 ctttgttctt tggttgcttt ttgacgccaa atacttgccc atgattcaaa aaattcttta
    18541 tagtttggat tttttcagtc ttaagagctt ctagagatgc cataacacca ccattatctg
    18601 caatattttc tgctaaagtt aatttaccat caactttacc gccatctgct ttaaggccat
    18661 cccattggtc aatcatagct tgtgtttttt tcttataatg ttttaaatct tctttagtcc
    18721 accaatcgtg aagattccct ttctcgtcat atttcatacc attaatatca aatgagtgtg
    18781 aaatttcatg accaataatt gctccgatag ctccataatt ttgactaact gttttagttt
    18841 tatcgtagag tggtgattga aaaatcgctg ctggaaagac tatggaattg gtattaggat
    18901 cattataagc atttacagca ttggcactca tttgccaatg ttcacgttga ttagactggt
    18961 taaattcttc aaatgttttc ttgttcgata attttctgta gttatcattg ttttcaaaga
    19021 agcttgcttt actatcaaat tggtactgac gataaagatc aggataatct tctggatagc
    19081 caatcattaa tctcatgtta tcaagtttct taatggccat tgcttttgta ttttctgata
    19141 accaagtgtt gttttttaac cgtgttttat agacattaat gattttctta gccatatgtt
    19201 cgacgtcttt tttagctgcc tcaccaaaat atttcttacc ataataatca cccaaaatat
    19261 ggccaaaagt atattctaat tgggtgtaag cattttttct ttcgattcaa tctgagtcaa
    19321 accagacgca acattcttaa aagcactcgc tgcttgacga ttttgttctc ctagaaaatc
    19381 tgattgatca actagcatag aaatcatcat ccatgctttc atattggcta attgtttact
    19441 atttataaca tcattaacct gtttaaaata agttttatct tctacattga ctgccttatt
    19501 cgttggccca actagttttt caatcaattc tttaaagtca aattccttgg cgtaattgtg
    19561 aaccgtttcc atactttcag ggttatatcg ccccgcagct gtctcacttt cagctgtgat
    19621 tttactttga tcaacttgcg ttttttctga taagagtcta tcaaatgcta tagcttgttt
    19681 aactagtttt ctatcttcaa tgttactttt tccagcttgt ttaagcaaag ccattgctga
    19741 agtacggtaa gctgataatt tagcctcacc ttctttattg cccttcttat attggtcagg
    19801 tgattcaagt aatgcgggtg cttgacgaag caccaattgc ttttggctat tatctcgagc
    19861 attggtttcc acagtcaaac caaatggtaa aacaaaacca ctcatcacaa aatcatgggc
    19921 caaactttga aagtctttca ttgaagagac tgcttcaagt ttttgtaaaa ctggttttag
    19981 aggtttgaga ccatttttat ctcttgtttt aaagtccata ccttgtttat agtatgcaac
    20041 catttttttc tgttcatcat tggttgtttc aatctttcct gaggccattt tagccatatc
    20101 agacactaac agttgcttta atttatcctc aatttctgaa aaactattaa cactagtctg
    20161 cccttgtttg agttttgtat tggctagcca attatcattg acagccctgt agagattttc
    20221 tctcggactg acattatttg ctttcttctc actggcagat actgcatgta acatagcaat
    20281 atctttaacc acatatcctg atataagaca agctgttaaa gtaagaacac tcatggattt
    20341 taaataggat tttctttgca taaatgacac ctctctttct tattcacttt tagtatagca
    20401 agtagggtgt taaattcttg ttaagaattc acttttttac acttatacaa aaaagtagta
    20461 gctaatgagc tactactttt gattt
//
LOCUS       DATRMA010000032        20112 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.032, whole genome shotgun sequence.
ACCESSION   DATRMA010000032 DATRMA010000000
VERSION     DATRMA010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20112
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.032"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            15..2300
                     /locus_tag="VBC28_001107"
     CDS             15..2300
                     /locus_tag="VBC28_001107"
                     /EC_number="3.4.14.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001270186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidyl-peptidase"
                     /protein_id="HEO6815099.1"
                     /translation="MRYNQFSYIPTKPNEAFEELKGLGFPLNKKNSDKANLEAFLRHS
                     FLNQTDTDYALSLLIVDAKTDALTFFKSNSDLTLENLQWIYLQLLGFIPFVDFKDPKA
                     FLQDINFPVSYDNIFQSLHHLLACRGKSGNTLIDQLVADGLLHADNHYHFFNGKSLAT
                     FNTNQLIREVVYVETSLDTMSSGEHDLVKVNIIRPTTEHTIPTMMTASPYHQGINDPA
                     ADQKTYQMEGALAVKQPKHIQVDTKPFKEEVKHPSKLPISPATESFTHIDSYSLNDYF
                     LSRGFANIYVSGVGTAGSTGFMTSGDYQQIQSFKAVIDWLNGKVTAFTSHKRDKQVKA
                     NWSNGLVATTGKSYLGTMSTGLATTGVEGLKVIIAEAAISTWYDYYRENGLVCSPGGY
                     PGEDLDVLTELTYSRNLLAGDYIKNNDCYQALLNEQSKAIDRQSGDYNQYWHDRNYLT
                     HVNNVKSRVVYTHGLQDWNVKPRHVYKVFNALPQTIKKHLFLHQGQHVYMHNWQSIDF
                     RESMNALLSQELLGIDNHFQLEEVIWQDNTTEQTWQVLDAFGGNHQEQIGLGDSKKLI
                     DNHYDKEAFDTYCKDFNVFKNDLFKGNNKTNQITINLPLKKNYLLNGQCKLHLRVKTS
                     DKKAILSAQILDYGPKKRFKDTPTIKFLNSLDNGKNFAREALRELPFTKDHYRVISKG
                     VLNLQNRTDLLTIEAIEPEQWFDIEFSLQPSIYQLSKGDNLRIILYTTDFEHTIRDNA
                     SYSITVDLSQSYLTIPTNQGN"
     gene            2304..2663
                     /locus_tag="VBC28_001108"
     CDS             2304..2663
                     /locus_tag="VBC28_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6815100.1"
                     /translation="MKLLTKERFDDSQHFWYQINLLQESNFGAVFDHDNKNIPQVVAT
                     IVDDLQGSGSSNYFWYFGNTTDTSILMIAHLNRKFYIQVNLKDFDFALNLIAINNWKS
                     LLQTQLEALNDTLAIFQ"
     gene            2709..3689
                     /locus_tag="VBC28_001109"
     CDS             2709..3689
                     /locus_tag="VBC28_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEO6815101.1"
                     /translation="MSSYWNNYPELKKNIDETNQLIQERIQVRNKDIEAALSQLTAAG
                     GKQLRPAFFYLFSQLGNKENQDTQQLKKIAASLEILHVATLIHDDVIDDSPLRRGNMT
                     IQSKFGKDIAVYTGDLLFTVFFDLILESMADTPFMRINAKSMRKILMGELDQMHLRYN
                     QQQGIHHYLRAISGKTAELFKLASKEGAYFGGAEKEVVRLAGHIGFNIGMTFQILDDI
                     LDYTADKKTFNKPVLEDLAQGIYSLPLLLAIEENPDIFKPILDKKTDMATEDMEKIAY
                     LVVSHRGVDKARHLARKFTEKAISDINKLPQSSAKKQLLQLTNYLLKRKI"
     gene            complement(3774..5522)
                     /gene="cydC"
                     /locus_tag="VBC28_001110"
     CDS             complement(3774..5522)
                     /gene="cydC"
                     /locus_tag="VBC28_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000472828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductant ABC exporter subunit CydC"
                     /protein_id="HEO6815102.1"
                     /translation="MFKIPLFKELKTDQWIKPFFKQYKVSLVIALFLGFMTFFSASAL
                     MFNSGYLISKSASLPSNILLVYVPIVLTRAFGIGRPVFRYIERLTSHNWVLRMTSQLR
                     LKLYHSLESNAIFMKRDFRLGDVMGLLAEDINYLQNLYLRTIFPTIIAWILYSFIIIA
                     TGFFSLWFALMMLLYLAIMIFLFPLWSILANGARQTREKELKNHLYTDLTDNVLGISD
                     WIFSQRGQEYVALHERSESELMAVQKKIRSFDNRRALIVELVFGFLAILVIIWASNQF
                     IGHRGGEANWIAAFVLTVFPLSEAFAGLSAAAQETNKYSDSIHRLNELSETYFETTQN
                     QLPNKPYDFSVKNLSFQYKPQEKWVLHHLDLDIKEGEKIAILGRSGSGKSTLASLLRG
                     DLKASQGEITLGDADVSIVGDCISNYIGVIQQAPYLFNTTLLNNIRIGNQDASEEDVW
                     KVLERVGLKEMVTDLSDGLYTMVDEAGLRFSGGERHRIALARILLKDVPIVILDEPTV
                     GLDPITEQALLRVFMKELEGKTLVWITHHLKGIEHADRILFIENGQLELEGSPQELSQ
                     SSQRYRQLKASDDGDL"
     gene            complement(5515..7233)
                     /gene="cydD"
                     /locus_tag="VBC28_001111"
     CDS             complement(5515..7233)
                     /gene="cydD"
                     /locus_tag="VBC28_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductant ABC exporter subunit CydD"
                     /protein_id="HEO6815103.1"
                     /translation="MLDKAVMRLSGIHKLLGLLAGLDVLQAIFIIGQAYYLSLSITGL
                     WEGQKLSSQTVYILLFMVSYLGRHVIDYIKNRKLDDFSTAQSSLLRRQLLDKLFDLGP
                     KVVQEQGTGNVVTMALDGVSLVENYLRLVLNKMINMSIIPWIILAYIFYLDIESGAIL
                     LIVFPLIIIFMIILGYAAQAKADKQYESYQVLSNHFLDSLRGIDTLKYFGLSKRYGKS
                     IYQTSESFRKATMSTLKIGILSTFALDFFTTLSIAIVAVFLGLRLLNEQIYLLPALTI
                     LILSPEYFLPVRDFSSDYHATLDGKNAFQAIQKVLNKTGIKGEQLVIDDWSKESRLDL
                     ENIAIAYDQKRVVEDVTLRFRGHQKVALVGVSGSGKSSLINLLSGFLGPDNGSLKVDG
                     REVTNLDQEDWHKQMIYIPQTPYVFEMSLRDNITFYTPNASDEEVVRAIHMVGLDSLL
                     SELPDGLETRIGNGARPLSGGQAQRIALARAFLDQNRRIMVFDEPTAHLDIETELELK
                     EKMLPLMSDRLVIFATHRLHWLNQMDVIVVMEKGRVAEVGSYQELLAKKGYLYQLKHA
                     MGGNDV"
     gene            complement(7233..8252)
                     /gene="cydB"
                     /locus_tag="VBC28_001112"
     CDS             complement(7233..8252)
                     /gene="cydB"
                     /locus_tag="VBC28_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001275291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HEO6815104.1"
                     /translation="MSALQFFWFFLIGLLFSGFFFLEGFDFGVGMAVQTLTHNEHEKD
                     QVVETIGPVWDGNEVWLLTGGGAMFASFPYWYASLFSGYYLILLTILFGLIIRGVSFE
                     FRHKVPAEKKQFWNWTLTIGSAIVPFFFGIMFISLIQGMPLDASGNLSAQFSDYFNIF
                     SLVGGVAMVLLAYLHGLNYIALKTEGPIRERARNYAQLLYWVLYLGLALFAVLLYFKT
                     DFFSNHPIVTTIMVLVIVVLAVLAHASTFKGAEMTAFLASGLSLVSVVVLLFQGLFPR
                     VMISSISPKYDLLIQNASSTPYTLKVMSIVAITLVPFVLAYTAWAYYIFRKRITLPVI
                     VTGEK"
     gene            complement(8253..9680)
                     /locus_tag="VBC28_001113"
     CDS             complement(8253..9680)
                     /locus_tag="VBC28_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HEO6815105.1"
                     /translation="MTIETLARFQFAMTTVFHFFFVPFTIGTCLVVAIMETMYVITKN
                     EEYKKLTKFWGNIMLLSFAVGVVTGIIQEFQFGMNWSDYSRFVGDIFGAPLAIEALLA
                     FFMESTFLGLWMFTWDNKKISKKLHVTFIWLVVFGSLMSAMWILTANSFMQHPVGYEV
                     VNGRAQMTDFLALVKNPQFFYEFTHVIFGAITMGGTVVAGMSAFRLLKSEQLKDTTVE
                     LYKKSVRIGLVVALLGSISVMGVGDLQMKALIHDQPMKFAAMEGDYEDSGDPAAWSVV
                     AWANEAEHKQVFGIKIPYMLSILSYGKPSGSVKGMDTANKELVAKYGKDNYYPMVNLL
                     FYGFRTMAAMGTAIMGVSVLGLFLTRKKKPILYKHKWMLWIVALTTFAPFLANTFGWI
                     VTEQGRYPWTVYGLFKIKDSVSPNVSVASLFVSNTVYFLLFGGLAVMMISLTIRELKK
                     GPEYEDEHGHHGAYTSIDPFEEGAY"
     gene            complement(9783..10991)
                     /locus_tag="VBC28_001114"
     CDS             complement(9783..10991)
                     /locus_tag="VBC28_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEO6815106.1"
                     /translation="MKEILVLGAGYAGLKAVRNLQKQSGDFHITLVDMNDYHYEATEL
                     HEVAAGSQPKEKITFPIKDVINTNKVNFMQDEVLRVDAENKTVTVKNNGELHYDYVVV
                     ALGFVSETFGIKGAMENALQMTNISQAENIHNHIVNTMKLYRETKDENLLKLLVCGAG
                     FTGIELAGAMVDERPKYAALAGVKPEQIEIICVEAATRILPMFDDELAQYGVNLIKDL
                     GINLMLGSMIKEIKPGEVVYGTSKEDEELKSITAGTIIWTTGVSGSPVMGESGFDQRR
                     GRVMVNSDLRDPKYDNVYVIGDVSAFMDTESGRPFPTTAQIATRMGAHVAKNLLHQIK
                     GEATEDFSYSPQGTVASVGNTHGLGVVGKTKIKKYPASVMKKIIMNKSLVDMGGLKEL
                     LAKGRFDLYH"
     gene            complement(11004..11903)
                     /locus_tag="VBC28_001115"
     CDS             complement(11004..11903)
                     /locus_tag="VBC28_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HEO6815107.1"
                     /translation="MTLPIFLELVEMKAKTASVLPFLIGLCFSAYYYNSVHPVYVGLF
                     FVAMFLFNMFVDIWNNYNDYRNAVDLDYKNDTNIIGRENLSLRQIEVIMASLVITSSM
                     IGLVLVSQVGLPLLWMGLFCFGIGVLYSFGPRPLSSLPLGEVFSGLTMGFMISLICVY
                     LNTYQNFSWDILNLSKIFLISLPNTLWIANLMLANNLCDKEEDEKNHRYTLVHYTGIR
                     GGLLLFAISNSIALLAIVFEFLFGLAPVTVLLSLLLIPFIYKQTKLLWQKQVKRETFV
                     CAVRILALGSATQVLTYLIGIIF"
     gene            complement(12312..12758)
                     /locus_tag="VBC28_001116"
     CDS             complement(12312..12758)
                     /locus_tag="VBC28_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6815108.1"
                     /translation="MNKLITEKKVNNVSTANYLKLGLVSAMFAGGAFVALGYTQGVSA
                     STFTAPQATPPKAERQLTDSELYERAQKQVLPRYIQGSLSGILNQHSTLYKQQNAAVT
                     PQVSSPKSERQLTDSEIYERAQKQVLPNYIQGSLSGILNQHSTLNA"
     gene            13641..13940
                     /locus_tag="VBC28_001117"
     CDS             13641..13940
                     /locus_tag="VBC28_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEO6815109.1"
                     /translation="MMNMQNMMRQAQKLQKQMEQKQADLAASQFTGKSAQELVTVTFT
                     GDKKLISIDYKEAVVDPEDIETLQDMTTQAINDALSQVDDATKKIMGAFAGKMPF"
     gene            14100..15290
                     /locus_tag="VBC28_001118"
     CDS             14100..15290
                     /locus_tag="VBC28_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEO6815110.1"
                     /translation="MVMDSLIIKQLIKSTFDIPLQVTYPNGNIETYNGSNPHVKLKLN
                     KNFSVSELSKDPSIVLGEAVMDGDIEIYGSIQELILSAYRCGDSFLRNSKFSKLIPKQ
                     FHDKKHSKSDIQKHYDIGNDFYKLWLDDTMTYSCAYFKHENDSLEQAQLNKVHHILNK
                     LNAQPGGKLLDIGCGWGTLIITAAKEYGLNATGITLSEEQASFITKRIKEEGLENKVT
                     VLIKDYRDIRETYDYITSVGMFEHVGKENLSQYFQTISKRLNINGLALIHGITGQVGG
                     NHGSGTNSWINKYIFPGGYIPRLTENLNHIASAGLQIADLEPLRRHYQKTLELWTKNF
                     HNALPEVQKTHDKRFINMWDLYLQSCAASFESGNIDIFQYLLSKGVSKDTMPMTRDYM
                     YSAN"
     gene            complement(15360..16085)
                     /locus_tag="VBC28_001119"
     CDS             complement(15360..16085)
                     /locus_tag="VBC28_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001292015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEO6815111.1"
                     /translation="MSEKYSTGDLAKEAGVTVRTVQYYDKRGILSPSELSEGGRRVYS
                     IADLEKLRQIIYLRDLDFSIDNIKNLFTEDNASQILELFLQVQIRELRLAIDSKKDKL
                     DKAVNLLKTVEKQDSKTLGYLSDIVLMEENKRKWGILQKSIVLQVVMIIMVYITLIIA
                     AVSLNQRWLMWIAVTVFLIAMNGMVWYFKQQVVYLCPNCHQTFTPTYQAFNLAKHTPK
                     TRKVFCPHCHQKSHCIELAREER"
     gene            complement(16212..16799)
                     /locus_tag="VBC28_001120"
     CDS             complement(16212..16799)
                     /locus_tag="VBC28_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEO6815112.1"
                     /translation="MKQLQEYIAFDLEFNTVGEHSHIIQVSAVKYSNHQEIALFDTYV
                     HTKVPLQSFINGLTGITARDIIGAPKIEIVLTDFQSFVGDTPLIGYNGYKSDLPLLVE
                     NGLDLTSQYQVDLYDEAFVRRSTDLNGIVNLKLTTVADFLGIKGKAHNSLEDARMTAR
                     VYEKFLDLDENKIYLKQQKEVAVDSPFATLGNLFD"
     gene            complement(16843..17379)
                     /locus_tag="VBC28_001121"
     CDS             complement(16843..17379)
                     /locus_tag="VBC28_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017766787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HEO6815113.1"
                     /translation="MTVEKTVSELAEILGVSRQAMNNRVKTLPEECVEKNSKGVTVVN
                     RDGLIKLEEIYKKTILEEEPIDEEASRRELLEILVDEKNTEITRLYEQLKAKDIQIAS
                     KDEQLHVKDIQIAEKDKQLDQQQQLTLTAMEDTQRLQLELNEAKAEVEEIQEAKEEKI
                     QELEAVKKSFFGRFFNKK"
     gene            17514..18686
                     /locus_tag="VBC28_001122"
     CDS             17514..18686
                     /locus_tag="VBC28_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEO6815114.1"
                     /translation="MKHYDTIIIGGGPAGMMAAISSNFYGNKTLLIEKNKRLGKKLAG
                     TGGGRCNVTNNGNLDELLAGIPGNGRFLYSVFSQFDNHDIINFFQDNGVTLKEEDHGR
                     MFPTTDKSRTIINALENKIKELGGQIMTDTEVVSVKKIGDSFYIKTKDTQFASDKLIV
                     TTGGKSYPSTGSTGFGHDIARHFKLEVTDMEAAESPLLTDFPHKKLQGISLDDVTLSF
                     EKHIITHDLLFTHFGLSGPAALRISSFVKGGETIYLDVLPNISVKELEIHFQNEREKS
                     LKNALKILLPERLAEFYAEDLPEKVKQVSVKDLEMLIQKLKKLPILVTGKMSLAKSFV
                     TKGGVDLKEINPKTLESKKVAGLHFAGEVLDINAHTGGFNITSALCTGWVAGSLHY"
     gene            complement(18730..19512)
                     /locus_tag="VBC28_001123"
     CDS             complement(18730..19512)
                     /locus_tag="VBC28_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017766788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4931 domain-containing protein"
                     /protein_id="HEO6815115.1"
                     /translation="MCYNNSMNERYIRFNPNIAKQKPSNKDKCPFCDKSQLGKILDVK
                     DDMIWVENKYPTLEETYQTLVIESNDHNGDISVYSESKMRQLLDYLLSKWQLMEESGH
                     YRSVVLYRNFGPLSGGSLRHPHSQIVGLKNVDAYETLNLDWFDGIEVCSETSQQAEIS
                     IANRPMLDFTEFTIKVSKLENAILIADSLPILCHYILNEFLGGRCKSYNLFFYKHDGG
                     LICKIVPRFIVSPYQIGYAIHQVNLPDELEKIKEALRAKYYL"
     gene            19671..19940
                     /gene="rpsN"
                     /locus_tag="VBC28_001124"
     CDS             19671..19940
                     /gene="rpsN"
                     /locus_tag="VBC28_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001085658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEO6815116.1"
                     /translation="MAKKSKIAKFQKQQKLVEQYAELRRELKEKGDYEALRKLPKDSN
                     PNRLKNRDLIDGRPHAYMRKFGMSRINFRNLAYKGQIPGIKKASW"
BASE COUNT         6838 a         3889 c         3156 g         6229 t
ORIGIN      
        1 agaaaggata tattatgcgt tataatcaat tcagctacat tccaactaaa cctaacgaag
       61 cttttgaaga gctcaaagga ctaggttttc cattaaacaa aaagaattct gataaagcta
      121 atttggaagc ttttctcaga cattcttttt taaatcaaac tgatactgat tacgctctta
      181 gtctccttat cgttgatgca aaaaccgatg ctctaacctt ttttaaatca aatagtgact
      241 taacactaga aaatttacaa tggatttatt tacagttatt aggctttatc ccttttgtag
      301 actttaaaga ccctaaagca ttcttacaag atattaactt cccagtctca tatgacaata
      361 tttttcaaag tctacatcac ttactcgcct gtcgtggaaa atctggcaat acattaatcg
      421 accaattagt tgctgatggt ttacttcatg cagataatca ctaccatttt ttcaatggga
      481 agtctctggc cactttcaat actaaccaat tgattcgcga agttgtctat gttgaaacat
      541 ccttagatac tatgtctagt ggtgaacatg atttagtaaa agttaacatt atcagaccca
      601 ctaccgagca tactatccct acgatgatga cagctagccc ctatcatcaa ggtatcaatg
      661 atcctgccgc agaccaaaaa acataccaaa tggagggtgc gctagcagtt aaacaaccta
      721 aacacataca agttgacaca aaaccattta aagaagaagt aaaacatcct tcaaaattac
      781 ccatcagccc tgcaactgaa agcttcacac acattgacag ttatagtctc aatgactatt
      841 ttctttctcg gggttttgct aatatatacg tttcaggtgt gggtactgct ggctctacgg
      901 gtttcatgac cagtggggat taccaacaaa tacaaagctt taaagcagtc attgattggt
      961 taaatggtaa ggttactgca ttcacaagtc ataaacgaga taaacaagtc aaggctaatt
     1021 ggtcaaatgg ccttgtagca accacaggta aatcttatct tggtaccatg tcaactggtt
     1081 tagcaacaac tggcgttgag gggctgaaag tcattatcgc tgaagccgca atctccacat
     1141 ggtatgatta ttatcgagaa aatgggcttg tgtgtagtcc aggcggctac cccggtgaag
     1201 atttagacgt tttaacagaa ttaacatact cacgaaacct cttagctggt gattacatca
     1261 aaaacaacga ttgctatcaa gcattgttaa atgaacaatc aaaagcaatt gaccgtcaaa
     1321 gtggggatta caaccaatac tggcatgacc gtaattacct aactcacgtc aataatgtca
     1381 aaagtcgagt agtttacact catggactac aggattggaa tgttaagcca agacatgtct
     1441 ataaagtttt caatgcattg cctcaaacca tcaaaaaaca ccttttttta catcaaggtc
     1501 aacatgtgta tatgcataat tggcagtcga ttgattttcg tgaaagcatg aatgccttac
     1561 taagccaaga actacttggc attgacaatc atttccaatt agaagaggtc atttggcaag
     1621 ataatactac tgagcaaact tggcaagttt tagatgcttt cggaggaaac catcaagagc
     1681 aaattggttt aggtgatagt aaaaaactta ttgataacca ttatgacaaa gaagcctttg
     1741 atacttattg taaagacttc aatgtgttca aaaatgatct tttcaaggga aataataaaa
     1801 ccaatcaaat cactattaat cttcctctaa agaaaaatta tctcctgaat ggacagtgca
     1861 aactccatct acgtgttaaa actagtgaca aaaaggccat tttatcagcc caaatcttag
     1921 actatggtcc taaaaaacga ttcaaagata caccaaccat caaattctta aacagccttg
     1981 ataatggtaa aaattttgcc agagaagctt tacgtgaact cccgtttact aaagatcatt
     2041 atcgtgtcat cagtaaaggt gtcttgaacc ttcaaaatcg tacagactta cttacaattg
     2101 aggctatcga gccagaacaa tggtttgata tcgagtttag cctccaacca agtatatatc
     2161 aattgagtaa aggtgataat ctaaggatta tcctttatac aactgatttt gaacatacca
     2221 ttcgagataa tgctagttac tctataacag tagatttaag tcaatcttat ttaactatcc
     2281 caactaatca aggaaattaa cttatgaaac ttctcactaa agaacggttt gatgattctc
     2341 aacacttttg gtaccagatc aatttattac aagagagtaa cttcggagca gtttttgacc
     2401 atgataataa aaacattcca caggttgttg caactattgt tgatgattta caaggttccg
     2461 gaagttcgaa ttatttctgg tattttggca atactactga tacttccatc cttatgattg
     2521 ctcatttaaa tcgaaaattc tatattcagg ttaatttaaa ggactttgac tttgcactca
     2581 atttaatagc tataaataat tggaagagtc tcctccaaac tcaactcgaa gctctaaacg
     2641 ataccctagc aatatttcaa taaataaggt agaatggagt gacaaagcaa cgcgagggag
     2701 actgattaat gtcatcttat tggaataact atcctgaact taaaaaaaat attgatgaaa
     2761 ccaatcaact aattcaagaa agaatacagg tcagaaataa agatattgaa gcggcgctaa
     2821 gccaactcac agctgcggga ggaaaacagc tcagaccagc attcttttac cttttttctc
     2881 aacttggtaa taaggagaat caagatactc agcaactaaa gaaaatcgct gcttctttag
     2941 aaatccttca cgttgctaca ttaatccatg atgatgtcat tgatgactca ccactaagac
     3001 gtggaaatat gaccattcaa agcaagtttg gcaaagacat cgcagtttac actggggatt
     3061 tacttttcac agtctttttc gatcttattt tagaatctat ggctgataca ccatttatga
     3121 ggattaatgc aaaatctatg cgtaaaattc tcatgggaga attggaccag atgcaccttc
     3181 gttacaatca acaacaaggt atccatcact atttacgtgc gatttcaggt aagacagccg
     3241 aactctttaa attagctagc aaagaaggag cttactttgg tggtgcagag aaggaggttg
     3301 ttcgtctagc aggccatatc ggctttaaca ttggtatgac attccaaatt ttggatgata
     3361 tcctggatta tactgcagat aaaaaaacat ttaacaagcc tgtcttagag gatttagcac
     3421 aaggcattta cagccttcct ctacttcttg ccattgaaga aaatcctgat attttcaaac
     3481 ctattttaga taaaaaaaca gatatggcta ctgaagacat ggaaaaaatt gcttatctcg
     3541 tcgtttccca tagaggtgtt gacaaagctc gccatctagc tcgtaaattt actgaaaaag
     3601 ctattagtga cataaataag ctaccccaga gctctgcaaa aaaacagttg ctacaattaa
     3661 ctaattacct tttaaaacgc aaaatttaaa taataaaaaa acattccaca atgctagaaa
     3721 agcagttagg gaatgttttt ttattatcat ttatttatcg cacctatcaa tcatcataga
     3781 tcaccatcat cagaggcttt cagctgacgg taacgttggc tactttgaga caattcttga
     3841 ggagaacctt ccaactctaa ttgcccattt tctataaata agatacgatc agcatgttca
     3901 atacctttta agtgatgtgt aatccaaact aaggtcttac cttccaattc tttcataaat
     3961 acccttagta aggcttgttc agtaatagga tcaagtccaa cagttggctc atctaagata
     4021 acaattggga catcttttag taagattcta gccaaagcaa ttctatgcct ttcgccacct
     4081 gaaaacctaa gtccagcttc atcaaccatt gtatagagac catctgataa atcagtgacc
     4141 atctctttca atccaactcg ttcaagaact ttccatacat cttcttcact agcatcttgg
     4201 tttccaatgc gaatgttatt tagcagggtt gtattaaaaa ggtagggcgc ttgttgtatc
     4261 actccaatat agttagaaat gcaatcacca actattgaaa catcagcatc gcctagggta
     4321 atctcccctt gacttgcttt caagtcgcca cgaagtagac tggctaaggt actcttgcca
     4381 gaaccactac gccctaaaat agcaattttt tctccttctt taatatccaa atctaaatga
     4441 tgcaaaaccc atttctcttg tggcttatac tggaaactta aattcttgac ggaaaaatcg
     4501 tatggcttat taggcaactg attttgagtt gtttcaaaat acgtttctga taattcatta
     4561 agacgatgaa ttgaatcaga atatttattt gtttcttgag cagcagcaga caaaccagca
     4621 aatgcctctg acaagggaaa tactgttaga acaaatgctg caatccagtt agcttcgcca
     4681 cctctgtgac caataaactg attactagcc caaataataa ccaaaatagc tagaaagcca
     4741 aaaactaatt ccacaataag agctctacga ttatcaaagg aacgaatttt tttctgaaca
     4801 gccatgagtt cactttccga acgctcatgc aaggcgacgt actcttgacc acgttgacta
     4861 aaaatccagt ctgaaatacc taagacatta tccgtcaaat cagtataaag atgatttttt
     4921 aattcttttt cacgagtctg acgagcacca ttagctaaaa tcgaccaaag tggaaaaaga
     4981 aaaatcataa tagctaaata aagcaacatc attaaagcaa accatagaga aaagaagcct
     5041 gttgcaataa taataaagct atataatatc cacgctataa tcgttggaaa tattgtccta
     5101 agatacaaat tttgcaaata attaatatcc tccgctaaaa gccccataac atctcctaaa
     5161 cgaaaatcac gcttcataaa gatagcattg ctttctaagg aatggtaaag cttcaagcgc
     5221 agctgtgaag tcattcgtaa tacccaattg tgactcgtta ggcgttctat ataacgaaaa
     5281 acaggacgtc caatgccaaa agcacgtgtt agtacaattg gaacatatac tagaagaata
     5341 ttactcggta gtgaagctga cttactaatc agatagcccg aattaaacat aagagcactg
     5401 gcagaaaaga aagtcataaa accaaggaaa agagcaatta ctaaggaaac tttatattgt
     5461 ttgaaaaatg gttttatcca ttgatctgtc tttaattctt taaacaaagg gattttaaac
     5521 atcatttcct cccatagcat gcttcagttg atagagatat ccttttttag ctaataattc
     5581 ctgataagag cctacttctg caactctccc cttctccata acgactataa catccatttg
     5641 gttcaaccag tgaagacgat gagttgcaaa gataactaaa cgatctgaca tcagtggcaa
     5701 cattttctct tttaattcta actctgtttc aatatctaaa tgagcagttg gttcatcaaa
     5761 aaccataata cgacgatttt gatcaagaaa agcacgagcc agagctatac gctgggcttg
     5821 tcctccacta agcggacgcg ctccattacc aatacgcgtt tctaaaccgt caggtaactc
     5881 gcttagcaag ctatctaaac ctaccatatg aatcgctctt accacctctt cgtcactagc
     5941 attgggagta tagaaggtaa tattatcacg taatgacatt tcaaaaacat aaggagtttg
     6001 aggaatatag atcatctgtt tatgccaatc ttcttgatca aggttagtca cttctctacc
     6061 atccactttt aaagaaccat tatcaggccc taaaaaacca ctcagaaggt taattaagct
     6121 agacttacct gaaccactaa ctcctactaa agcaaccttt tgatgtcctc taaatcgtaa
     6181 ggttacatcc tcaacaacac gtttttggtc gtacgcaata gcaatgtttt ctaaatccaa
     6241 acgactctct ttagaccaat catctatgac taattgctca cctttaatac cagttttgtt
     6301 tagaactttt tgaatcgctt gaaatgcatt tttcccgtct aacgttgcat gataatcact
     6361 agaaaaatct cttacgggta aaaagtattc aggggataaa atcaaaattg ttaaagccgg
     6421 taaaaggtaa atctgctcat ttaataatcg taaacctaga aaaacagcaa cgatagcaat
     6481 ggataaagtt gtaaaaaagt ctaaagcaaa cgttgataaa ataccaattt tcaaagtact
     6541 cattgttgct ttacgaaaag attcactagt ttgataaatg cttttaccat aacgtttact
     6601 aagaccaaaa tatttgagag tatcaatgcc tcgtaatgaa tctaaaaaat gattagaaag
     6661 tacttgatag gattcatatt gtttatctgc tttagcttga gccgcatagc ctaaaataat
     6721 cataaagatg ataattaaag gaaaaacgat aagtaaaatt gcacctgatt caatatctaa
     6781 atagaaaata taagctagaa taatccatgg gataatagac atgttaatca tcttattcaa
     6841 gactaaacgt aaataatttt caactaaaga aacgccatct aatgccattg taacaacatt
     6901 accagttcct tgttcttgaa cgactttagg acctagatca aataacttat ctaataattg
     6961 acgtctcaaa agactagatt gtgctgttga aaagtcatca agttttctat ttttgatgta
     7021 atcaataaca tgacggccta aatatgagac cataaataac aaaatataaa ccgtttgaga
     7081 agaaagtttt tgcccttccc acaaaccagt tatagaaaga ctcaaataat aagcttgtcc
     7141 gataataaag attgcttgaa gaacatcaag tcccgcaagc aatcctaaca acttatgaat
     7201 acctgacaag cgcataaccg ctttatctag cattattttt ctcctgtcac aatcactggc
     7261 aaggtaatac gttttctaaa gatataatat gcccatgctg tataagctag aacaaaggga
     7321 actagagtaa ttgcgacaat cgacattact tttaaagtat atggcgttga ggatgcattt
     7381 tgaatcagca aatcatattt agggctaatt gatgaaatca taactcgtgg gaatagacct
     7441 tggaacaaca gaacaaccac tgaaacaaga ctgagaccac tcgctaaaaa tgctgtcatc
     7501 tctgctcctt taaaagttga agcatgcgct aatacagcta atacaacaat aaccaaaacc
     7561 ataattgtcg taactatcgg atgattgcta aagaaatctg tcttgaaata caagagaact
     7621 gcaaacaagg ctaaaccaag atagagtacc caataaagca actgagcata attacgggca
     7681 cgctctctaa ttggcccttc tgtcttcaat gcaatgtagt ttaaaccatg aagataggct
     7741 agcaatacca ttgcaactcc acccaccaac gagaatatat taaagtaatc actgaattga
     7801 gctgataagt taccactggc atcaagtggc ataccttgaa tcaaacttat aaacataatc
     7861 ccaaagaaga atggaacaat agcggaacca attgttaagg tccagttcca aaactgcttt
     7921 ttctctgcag gaactttatg tctaaattcg aaggaaacac cacgaataat taatccaaag
     7981 agaatcgtaa ggagaatcaa atagtaacca ctaaatagtg aagcatacca gtatggaaaa
     8041 gaagcaaaca ttgctccacc accggtaaga agccaaactt cattaccatc ccaaactggg
     8101 ccaatggttt ctactacttg atctttttca tgctcattat gtgttaaagt ttgaacagcc
     8161 atcccaaccc caaagtcgaa tccttcaagg aagaagaatc ctgaaaatag gaggccgatt
     8221 aggaaaaacc aaaagaattg taaagcactc atttagtatg ctccttcctc aaagggatca
     8281 attgaagtat atgcaccatg atgtccgtgt tcatcttcat actctggccc tttcttcaat
     8341 tctcttattg ttaggcttat catcatcacc gctaaaccac caaacaacaa gaaatacaca
     8401 gtattggaaa caaataatga tgcaactgaa acgtttggag agacagagtc cttaatcttg
     8461 aaaagaccat agacagtcca agggtatcgt ccttgttctg tcacaatcca accaaatgta
     8521 ttggctaaga atggtgcaaa ggttgttaaa gcaacaatcc aaagcatcca tttgtgttta
     8581 taaagaattg gttttttctt acgagttaaa aagagtccta aaacagaaac acccataata
     8641 gctgttccca tcgccgccat tgtacggaaa ccatagaaaa gtaggttaac cattggatag
     8701 tagttatctt taccatactt agcaaccaat tctttattag ctgtatccat acctttcaca
     8761 gaaccagatg gtttcccgta agataaaata ctcaacatat aaggaatctt aatcccaaaa
     8821 acttgtttgt gttctgcttc atttgcccaa gcaactacac tccaagctgc aggatcaccc
     8881 gaatcttcat aatcaccttc catagcagca aatttcattg gttgatcgtg aatcaaggct
     8941 ttcatttgta aatccccaac tcccataact gaaattgaac caagaagtgc tactactaaa
     9001 ccaatacgaa ccgatttctt ataaagttca accgttgtat ctttcagctg ttctgatttt
     9061 aagaggcgga aggctgacat accagcaacc actgtaccac ccatcgtaat ggcaccaaaa
     9121 ataacatggg taaattcata gaagaactgt ggatttttta caagtgcaag aaaatcagtc
     9181 atttgcgctc taccgtttac aacttcataa ccaacaggat gttgcatgaa gctattagca
     9241 gtaagaatcc acatcgctga cattaatgag ccaaaaacca ctagccaaat gaatgtcaca
     9301 tgtaattttt tactaatttt tttgttatcc catgtaaaca tccataaacc aaggaaagta
     9361 gattccataa agaaagctaa taaagcttca atagccaatg gagctccaaa aatatcacct
     9421 acgaaacgtg aatagtctga ccagttcatt ccaaattgga actcttgaat gatacctgtc
     9481 acaacaccaa ccgcaaaact aaggagcatg atattacccc aaaattttgt caatttttta
     9541 tactcttcat tttttgtgat aacatacatt gtctccataa tagcaaccac taggcatgta
     9601 ccaattgtaa atggtacaaa gaaaaagtgg aaaactgtag tcatcgcaaa ttgaaaacga
     9661 gctaaagttt caattgtcat acaaaacctc ttattttgtt ttaatcattt cttttataga
     9721 aatatctgca agagctagtt tcctagcttc gtaacaattg gtaaataact tactttaata
     9781 tcttaatgat ataaatcaaa acgtccctta gctagaagtt ctttaagtcc acccatatca
     9841 acaagtgatt tgttcatgat aatcttcttc ataaccgacg cagggtattt tttaatttta
     9901 gtttttccaa caacaccaag tccatgcgta tttcctactg aagcaactgt gccttgcgga
     9961 ctatacgaaa agtcttctgt agcttcacct ttgatttgat gtaaaaggtt tttagcaaca
    10021 tgagctccca tacgagttgc aatttgagct gttgttggga atgggcgacc agactcagtg
    10081 tccataaatg cagacacatc accgataaca taaacattat cgtattttgg atcacgcaaa
    10141 tcactgttga ccattacacg tccacgacgt tgatcaaatc ctgattctcc cataaccgga
    10201 cttccactaa cacctgttgt ccaaatgata gtacctgcgg taatagattt gagttcttca
    10261 tcctctttac tagtaccata aacaacttca cccggtttga tttccttaat catacttcca
    10321 agcatcaaat taatacctaa atccttaatt aagttgacac catattgcgc taattcatca
    10381 tcaaacattg gtaggatgcg agttgctgcc tctacacaaa taatttcaat ttgttcaggt
    10441 tttacacccg ctaaagcagc atattttggt cgctcatcaa ccatagctcc ggctaactca
    10501 atacctgtaa aaccagcacc acatactaag agtttgagaa gattttcatc ttttgtctca
    10561 cggtacaact tcattgtatt aacaatgtga ttatggatgt tttctgcttg cgaaatattg
    10621 gtcatctgca aagcattctc catagcaccc ttgatgccaa aagtttcaga tacaaagcct
    10681 aaggcaacta cgacataatc ataatggagt tcaccgttat ttttaactgt tactgtttta
    10741 ttttcagcat cgactctaag cacctcatct tgcatgaagt taaccttatt agtgttgata
    10801 acatccttaa ttgggaatgt aattttttct tttggctgag agcctgcagc tacttcatgt
    10861 aattctgtag cttcgtagtg ataatcattc atgtcaacaa gggtaatatg aaaatcacct
    10921 gattgttttt gtaaattacg aactgctttc aaaccggcat aaccagcacc taaaactagg
    10981 atttctttca tgataaaact cctctaaaaa atgattccaa ttagatacgt taagacttgg
    11041 gttgcagatc ccagtgctag aatacgtacc gcacaaacaa atgtttctct cttaacctgt
    11101 ttttgccaca atagttttgt ctgcttataa ataaatggta ttaataacaa actcaagagt
    11161 actgtaacag gcgcaagtcc aaaaagaaat tcaaaaacaa ttgccaataa agctatacta
    11221 ttagaaatag caaacagcaa gagtccacct cttatccctg tataatgtac aagggtatag
    11281 cgatgatttt tttcatcttc ttctttgtcg cacagattat tcgccaacat tagattggca
    11341 atccataagg tgttcggcaa ggaaatcaag aaaattttgc ttaagttgag gatatcccac
    11401 gaaaagtttt gataagtgtt tagatataca caaattaaac taatcataaa tcccattgtc
    11461 aagccagaaa atacttctcc taagggcaga ctagataaag gtcttggacc aaaggaatat
    11521 aaaacaccaa ttccaaaaca aaaaagtccc atccataaaa gaggcagacc gacttgggaa
    11581 actaaaacta aaccaatcat actagaagta atgactaaac ttgccataat aacttctatt
    11641 tggcgtaggg ataggttctc acgaccaata atgttagtgt cattcttata atccaaatcc
    11701 acagcattcc gataatcatt gtaattattc caaatgtcaa cgaacatatt gaataaaaac
    11761 atagcaacga agaataaacc gacatataca gggtgtactg aattgtagta ataagcacta
    11821 aaacacaaac caatcaaaaa tggtaaaaca ctagctgttt tggctttcat ttctaccaac
    11881 tctagaaaga ttggtaatgt cattattaaa gtcctctcta tacataatct tcaacaatac
    11941 tattataatg caatttctac catttttaaa acaatagggc ttgatttttg attaaaaata
    12001 caaaaaattt ttcttaaaat gagttaatca cacttagaac actaaatact attttgagga
    12061 gtcctattca tcacactatg ccatgtaata ttcggaaaaa tagtattgca accctcaact
    12121 atcccttatg ttggcaccag cctttgaaag tcctctgatt gcactgctta gttagtattt
    12181 tcatcagctg tatgatctct aagagccatc agcatactaa aaaaactagt attacacttt
    12241 gaaaagtgta acactagctc caatttgttt taaatagctt aactatttaa tcattaatct
    12301 actttatata tttaagcatt taaagttgaa tgttggttga ggatacctga gagagaacct
    12361 tgaatataat ttggcaatac ttgtttttga gcacgttcat agatttctga atctgttaac
    12421 tgacgttctg atttggggct tgatacttgc ggtgtcactg ctgcattttg ttgtttgtaa
    12481 agtgttgaat gctggttaag gatacctgag agagaacctt ggatatatct tggcaatact
    12541 tgtttttggg cacgttcata aagttctgaa tccgttaact gacgttctgc ttttggaggt
    12601 gttgcttgcg gtgctgtgaa agttgaagct gaaacacctt gtgtataacc aagagctacg
    12661 aatgctcctc cagcaaacat tgctgagaca agaccaagtt tcaaatagtt agcagttgag
    12721 acgttgttga cttttttttc tgtaataagt ttgttcataa gaattacctc tttcttttgg
    12781 gttatttatt ttatgaacta agtataacaa caagaaataa agtaaggtta aactaaatat
    12841 aaaataattc ttaagtcatt cttaggaaac cataaaatca tacactaaca aaaaatttgg
    12901 tatctagctc aaaagtaaaa gtagaataaa ggcttttact tctgagctag taatgaataa
    12961 attatatttg catctagttt ggaattagcc ctagtgtatt ataatgatcc ctacaaatat
    13021 gtgccaaaca ccttcttcat atatacaaga ttgatttaac attcctatac ccttaatggt
    13081 gggcacattc tcttcatgtt ccatatagaa atcaacagat aattccgtat tgtccctatt
    13141 attaaatttt ccagttaatg ataaattata tcatccacct tgaggtgtcc tattaccaat
    13201 ctgttcaacc tttacacttg acgaatcgaa ttgaacagat tctgtcttat cattctgtga
    13261 tttagaacat caacaatttc ttgttcatgt tctttgaggt aagctagttg ttcttcttta
    13321 acgtgctttt atctttcgta cttactgata cttgagagca tttttttgac cctacttctt
    13381 actaatcagc tgacgtccct ataagatgaa tcataaacac atagctgcca ctaaccattt
    13441 taacctttta tgcatacata attcttcttg atatcccata ccattatctt tagtataaac
    13501 acttaataaa acaagcattt aacaactaag gttaataaaa ttcaataaat ctaatccttc
    13561 tacttacttt agtttatcac ttttttctct catcctatgc tataatagta aggtatcaaa
    13621 atttaggaga atatagtctt atgatgaata tgcaaaatat gatgcgtcaa gcacaaaagc
    13681 ttcaaaaaca aatggaacaa aagcaagctg atttagcagc tagccagttt actggaaaat
    13741 ctgctcaaga gctcgttacc gtgacattta caggcgataa aaagctaatt tcaattgatt
    13801 acaaagaagc tgtggtagac cctgaagata ttgaaacact acaagacatg acaactcaag
    13861 ctatcaatga cgctttaagc caagttgacg atgctacaaa aaaaattatg ggagcatttg
    13921 cgggtaaaat gccattctaa aaaataaatc acatttactg aatcacctgt aaatgtgatt
    13981 tttatttcaa acttctttca ttttttcacg aaaacaagtt ttattttcaa aaaatatttg
    14041 caaaaaatca gcagagaggt ataataacat tgaaaatatt agagagaagg tgactaacca
    14101 tggttatgga tagtctaatc ataaaacagt tgattaagag tacatttgat atacccttac
    14161 aagtcactta tccaaatggg aatattgaaa cttataatgg tagcaatccc cacgtcaaac
    14221 ttaaactaaa caaaaatttc tccgtctcgg agctatcaaa agacccttcc atagtattgg
    14281 gtgaagctgt tatggatgga gacattgaaa tttatggttc tatacaagaa ttaattttat
    14341 cagcttaccg ctgtggagac agctttctac gtaatagtaa atttagcaaa ttgattccta
    14401 aacaatttca tgataaaaaa cacagtaaat ccgacattca aaagcattat gacatcggaa
    14461 atgatttcta taaactgtgg cttgatgata ccatgactta ttcatgcgcc tattttaaac
    14521 atgaaaatga tagtctagag caagctcaac taaacaaagt acaccatatt cttaataaat
    14581 taaatgctca gccgggaggg aaattactag acatcggttg tggctggggg acacttatca
    14641 ttactgctgc taaagaatat ggcttaaatg cgacaggtat caccttaagt gaagagcagg
    14701 cttctttcat tactaagcgt atcaaggaag aagggttgga aaataaagta acagtcctca
    14761 ttaaagatta tcgtgacatt cgtgaaacat acgattatat cactagtgtt ggtatgtttg
    14821 aacatgtagg aaaagaaaat ttatctcaat acttccaaac catttctaaa agactcaaca
    14881 tcaatggtct agctcttatt catggtatta ctggacaagt gggtggtaat catggtagtg
    14941 gaactaattc ttggattaat aagtatatct tcccaggagg atatatccct cgtttaactg
    15001 aaaatttaaa tcatatagct agtgctggac ttcaaattgc tgacttagaa ccgcttcgtc
    15061 gtcactatca aaagactctc gaattatgga caaaaaattt ccacaatgcc cttcctgaag
    15121 ttcagaaaac tcatgataag cgttttatca atatgtggga cctctatctt caatcttgtg
    15181 cggcctcttt tgaatctggc aatattgata tttttcaata cttgctttca aaaggtgttt
    15241 caaaagacac tatgcctatg acacgcgatt atatgtattc tgctaattga tatagttaat
    15301 attatagtat tccaacttat tctaaataaa aaggactctc ttgaattcaa gggagttctt
    15361 taacgttctt ctctggctaa ctctatacaa tgtgactttt ggtgacaatg tggacagaat
    15421 acttttcgcg tcttaggggt atgtttcgct agattaaatg cttgatatgt cggtgtaaag
    15481 gtttgatgac aatttggaca aagataaaca acctgttgtt taaaatacca aaccatacca
    15541 ttcatagcta ttaagaatac tgttactgct atccacataa gccatctttg atttagagaa
    15601 actgctgcta ttattaacgt aatatagacc ataataatca taacgacttg tagcactatg
    15661 cttttttgta gtatacccca ttttcttttg ttttcttcca taagcacaat gtcagaaaga
    15721 tagcctaatg ttttactatc ttgtttttct acagttttta gtaggttgac agctttgtca
    15781 agtttgtctt ttttactatc aatagctagc cttaattctc ttatctgcac ttgtaaaaaa
    15841 agctctaaaa tctggctagc attgtcttca gtaaatagat ttttgatatt atcaattgaa
    15901 aaatctaaat ctcgtaaata aataatttgt ctaagctttt ctaaatcagc aatactataa
    15961 accctcctac ctccttcaga tagttctgat ggggataaaa ttcctctttt gtcatagtac
    16021 tgaacagtac gaacagtaac tcctgcttct ttagctaagt caccagtgga atatttttct
    16081 gacatgtttc acacctcctt taatatagta aacacttctt tatcagaaac acctttacta
    16141 tatcaaatga cctaaggtaa taagcaagta ataacgctaa gtatttttaa ctttaaataa
    16201 aactacaaca attaatcaaa caaatttcct aaagtcgcaa atgggctatc aactgctacc
    16261 tctttttgct gttttaaata gattttattt tcatccaagt ccagaaattt ttcataaaca
    16321 cgcgcagtca tacgggcatc ctctaagcta ttatgtgctt taccctttat tcctaaaaag
    16381 tcagcaacag tagtcaattt aaggttgaca ataccattta aatctgtact gcgtctcaca
    16441 aaagcttcat catataagtc cacctgatat tgactagtta gatccaaacc attttcaact
    16501 aaaaggggta aatccgattt atacccatta taaccaatca gtggtgtatc tccaacaaaa
    16561 ctctgaaagt ctgtcaaaac tatctcaatt ttaggagccc ctataatatc tctagctgta
    16621 atacctgtta aaccattaat aaagctttgt aatggaactt ttgtgtgaac ataagtatca
    16681 aataaagcaa tctcttgatg attagaatat ttaacggccg acacttgaat aatatggcta
    16741 tgctcaccaa ccgtgttaaa ctctaaatca aaagctatat attcttgtaa ttgtttcatt
    16801 atttctccta aaaagcgcaa ggaaactcct tacgctttta ttctattttt tattgaagaa
    16861 acgtccaaag aaactttttt taacagcttc taattcttga attttttctt ctttggcttc
    16921 ttgaatctcc tcaacctctg ctttagcttc atttaactca agttgcagac gttgcgtatc
    16981 ttccatagca gttaaagtca attgctgttg ttgatctaat tgcttatctt tctctgctat
    17041 ttgaatatct tttacatgta actgctcgtc ttttgaagca atctgaatat cttttgcttt
    17101 taactgttca tataaacgtg tgatttcagt attcttctca tcaacgagaa tttctaaaag
    17161 ttctcgtctt gatgcttctt cgtcaattgg ttcctcttcc aaaatagttt ttttatagat
    17221 ttcttccaat ttaatcaagc catcacgatt aacaactgta acgcctttac tattcttttc
    17281 cacacattct tcagggagtg ttttaactct gttattcatc gcttgacgac taacccctaa
    17341 aatttcagct aattcactaa ccgtcttttc aactgtcata acttcctctt ttgtactgat
    17401 tttcttataa aagaatatca caatcctaat ctagtgtcaa tttactgtca acttaaagtg
    17461 tcaaacaaga cattttgacg tctatttctc tatttatatg ctagaataag attatgaagc
    17521 attatgacac aataattata ggtggtggac ctgctggaat gatggcagct atttcctcaa
    17581 atttttacgg caacaaaaca cttcttattg agaaaaataa acgtttaggc aaaaaactag
    17641 ctggaacagg tggtggccgc tgtaatgtta ccaataatgg aaaccttgat gagctcttag
    17701 ctggtatacc tggaaatggc cgtttcctat atagcgtctt ctcacaattt gataaccatg
    17761 atattattaa cttctttcaa gacaacggcg tcacactaaa agaagaagat catgggcgta
    17821 tgttccctac tactgataaa tctcgtacta tcattaacgc ccttgaaaat aaaataaaag
    17881 aacttggtgg tcaaattatg actgacacag aagttgtctc tgtcaaaaag ataggagatt
    17941 cattctatat caaaactaaa gatacacagt tcgcatccga taaacttatc gtaacaactg
    18001 gtggtaaatc ctatccgtct acaggttcta ctggtttcgg tcatgacatt gctcgtcact
    18061 ttaaattaga agtcacagat atggaagccg ccgaaagccc tcttttaact gattttcccc
    18121 ataaaaaact acaaggtatc tccttagatg atgtcacttt gtcatttgaa aagcatatta
    18181 ttacacacga tcttctcttt actcattttg gattatcagg accagcagcc ttgcgaattt
    18241 ctagctttgt caaaggcgga gaaactatat acctagatgt tttgcccaat atctctgtta
    18301 aagaattgga aatacacttc caaaatgaaa gagaaaaatc tttgaaaaac gctcttaaaa
    18361 tacttcttcc tgagcgttta gctgaatttt acgctgaaga cttacccgaa aaagtaaaac
    18421 aagtatctgt aaaagactta gaaatgttaa ttcaaaaact taaaaaactt cctattttag
    18481 taactggcaa gatgtctcta gcaaaatcct ttgtaacaaa agggggagtt gatcttaaag
    18541 aaataaaccc caaaacactt gaaagtaaaa aggtagctgg tttacatttt gctggtgaag
    18601 tcctagacat caatgctcat acgggagggt ttaacattac ttctgcctta tgtacaggct
    18661 gggtagctgg tagcttacac tattaataca tgaaagctga acaattccgt cagctttttt
    18721 atatagaatt taaagataat atttggctct taaggcttct ttgatttttt ccaattcatc
    18781 tggtaaattt acctgatgaa tagcataccc aatctgataa ggggatacta taaaacgagg
    18841 aacaatttta caaataagac ccccatcatg cttatagaaa aagagattat aagatttaca
    18901 gcgccctccc aagaattcat ttaaaatata atgacacaaa attggtaaac tatctgcaat
    18961 caaaatagcg ttttccaatt tggatacttt aattgtgaac tctgtgaaat caagcatagg
    19021 acgattggca atactaatct ctgcttgttg actcgtttcc gaacaaacct cgataccatc
    19081 aaaccaatct aagttcaacg tttcataagc atctacgttt tttaagccaa caatttggct
    19141 atgaggatgt cgtaaacttc cgccagataa gggcccaaaa tttcgataaa gaacgaccga
    19201 acggtaatga ccactttctt ccattaattg ccattttgac aaaagataat ctaacaattg
    19261 gcgcatcttt gactcactat aaactgaaat atccccattg tgatcatttg attcaatcac
    19321 caatgtttga taggtttctt ctaaagttgg gtatttattt tctacccaga tcatatcgtc
    19381 tttcacgtct aggatttttc ctaattggct tttatcacaa aatggacatt tgtctttatt
    19441 gctgggtttt tgtttcgcga tattagggtt aaatcggata tatctttcat tcatactgtt
    19501 attgtaacac attcatcacc attacaatat aatacttatc cagttaacat tttccttgca
    19561 ttcttagctg aaaagagtat aatagaatca tgtcttgttt actggttaat taccagtaaa
    19621 ctaggtaccc tgttttggga tagaaaaatc tttttgggaa aggagaaatc atggctaaga
    19681 aatcaaaaat tgctaaattt caaaaacagc aaaaactcgt agaacaatat gctgaactac
    19741 gtcgtgagtt aaaagaaaag ggtgattacg aagcacttcg taaacttcct aaagattcta
    19801 accctaaccg acttaaaaat cgtgatttaa tcgatggccg tcctcacgct tacatgcgta
    19861 aattcggtat gagtcgtatt aacttccgta acctagctta caaaggccaa attcctggca
    19921 ttaaaaaagc aagctggtaa ttcgtgaaga caaatagtta ctttttatga ctattctatt
    19981 gagtgtggac gaatagttaa tacattgtaa ttatttaacc tgttgagtat ttactcagct
    20041 attacaaact tcttcaaaat tttattatca taggaatgta ggatctcatc aatccttaaa
    20101 tgactaataa ta
//
LOCUS       DATRMA010000033        19848 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.033, whole genome shotgun sequence.
ACCESSION   DATRMA010000033 DATRMA010000000
VERSION     DATRMA010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19848
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.033"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            5..1197
                     /locus_tag="VBC28_001125"
                     /pseudo
     CDS             5..1197
                     /locus_tag="VBC28_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567306.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
     gene            complement(1446..1916)
                     /locus_tag="VBC28_001126"
     CDS             complement(1446..1916)
                     /locus_tag="VBC28_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEO6815117.1"
                     /translation="MVKDRQIQKTKVAIYNAFISLLQENDYSKITVQDVIGLANVGRS
                     TFYSHYESKEVLLKELCEDLFHHLFKQGRDVTFEEYLVHILKHFEQNQDSIATLLLSD
                     DPYFLLRFRSELEHDVYPRLREEYITKVDIPEDFLKQFLLSSFIETLKWWLHQR"
     gene            2048..2911
                     /locus_tag="VBC28_001127"
     CDS             2048..2911
                     /locus_tag="VBC28_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEO6815118.1"
                     /translation="MTSNKKVAIAFILNISFSVLEFIFGSLFFSGAILADAVHDFGDA
                     IAIGISATLEKKSKKDEDTIFSLGYKRFSLLGALITSLILISGSILVMIENIPKLWHP
                     TPVNYHGMFILAVIAIIINGLASFILHSGQSKHEEILSLHFLEDILGWLAIIVISLIL
                     NWKPLYILDPLLSVAISTFILSKALPKLLSTLKLFLDGVPDSIDYAALHDELKGLSQV
                     RSINQLNIWSMDGIDNRAIIHCCLNQLISEKDCKRAIRTICQHYKINDVTVEIDYSLR
                     EHQNHCKPLKN"
     gene            2996..3850
                     /locus_tag="VBC28_001128"
     CDS             2996..3850
                     /locus_tag="VBC28_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEO6815119.1"
                     /translation="MTDTMILNDNHKIPTLGLGTWMIDNDRVAQVVKDAIELGYRHID
                     TAQAYNNEKGVGVGIKESGITRESLFVTTKIAAEHKDYESATKSIDQSLEALGLDYID
                     LMIIHSPQPWKEWRETDKHFDQGNLEAWRALEDAQKSGKMKSIGVSNFLEKDLENILK
                     NGHVKPAVNQILAHIGNTPFDLIDYCQSKGIQVEAYSPIAHGQALKSDGIQKMAEKYG
                     VSVAQLCIQYLLQLNLIVLPKASSKEHLQSNLDFDFVISDEDMSILKSLMFDDYGEFS
                     NFPVFSGK"
     gene            3960..4997
                     /locus_tag="VBC28_001129"
     CDS             3960..4997
                     /locus_tag="VBC28_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HEO6815120.1"
                     /translation="MKVATFIEPGKMVITDTPKPVIEQETDAVIKIVRACVCGSDLWW
                     YRGISKRESGSFAGHEAIGIVEEVGTKVTDVSKGDFVIVPFTHGCGQCPSCKAGFDGN
                     CTNHQAAKNVGYQGQYLRYTNANWALVKIPGQPSDYDNETLNSLLTLSDVMATGYHAA
                     ATAEVKEGDTVVVMGDGAVGLCGVIAAKMLGANRIIAMSRHKDRQELALTFGATDIVE
                     ERGDEAVKRVLDLTNQAGADAVLECVGTEQSVDTATQIARPGAVIGRVGIPQNPDMNT
                     NNLFWKNIGLRGGIASVTTFDKSVLLDAVLTHKINPGLVFTKSFVLDDIQKAYEAMDK
                     RDAIKSLVIVD"
     gene            5013..5402
                     /locus_tag="VBC28_001130"
     CDS             5013..5402
                     /locus_tag="VBC28_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEO6815121.1"
                     /translation="MHIKEVSKKTGITSDTIRYYERINLIPPIHRSESGIREFTQNDI
                     DILEFVRYFRGAGVSVESLIDYIHLVEKGDSTIPARLEILKDERDNLEDRLQGLQEAL
                     NRLNHKIDNYQNKVVPREQELFDPTTK"
     gene            5412..5807
                     /locus_tag="VBC28_001131"
     CDS             5412..5807
                     /locus_tag="VBC28_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001158469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEO6815122.1"
                     /translation="MQETTKSIFPKGEKNPYGEFFIGQSYLAALAKSPDGNVSVGNVT
                     FEAGCRNNWHVHLDGYQILLVTEGSGWYQEEGKEAVSLKPGDVIVTDKGVRHWHGAKK
                     DSEFAHIAITAGKSEFYEAVSDEEYSRLG"
     gene            5831..6148
                     /locus_tag="VBC28_001132"
     CDS             5831..6148
                     /locus_tag="VBC28_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEO6815123.1"
                     /translation="MKEKQTAGRRQLEEFAPEFARYNDDILFGEVWAKEDHLTDKTRS
                     IITISALISGGNLEQLEHHLQFAKQNGVTKEEIADIITHLAFYVGWPKAWSAFNKAKE
                     IWI"
     gene            6235..6519
                     /locus_tag="VBC28_001133"
     CDS             6235..6519
                     /locus_tag="VBC28_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000955821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2316 family protein"
                     /protein_id="HEO6815124.1"
                     /translation="MLTIQEMKDTTFELQENFRRLNYPIKQVAKDLQLNISEVESLLS
                     LDVTYPGDVCMLRDYLEDMLKKEGKEVYPFSRLASHSANRWYPYETPWRY"
     gene            6758..7189
                     /locus_tag="VBC28_001134"
     CDS             6758..7189
                     /locus_tag="VBC28_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEO6815125.1"
                     /translation="MLSFQIVEKPAMILAGVTLENVKSNQEGIQQAIGICKTQPDFRF
                     DYSATYQVETSVQAPKGLEIIRIPSATYAVISVKGPMPSSLQETWRKIIQGFFQENNL
                     KPANSPNLEIYSSQHPQDTDYQMEIWLAIADITNNSKTNLI"
     gene            complement(7233..7622)
                     /locus_tag="VBC28_001135"
     CDS             complement(7233..7622)
                     /locus_tag="VBC28_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000632624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEO6815126.1"
                     /translation="MITQEMKEIINSQLAMVATVDAKGQPNIGPKRSMRLWDDKTFIY
                     NENTDGQTRINIEDNGKIEIAFVDRERLLGYRFVGTAEIQTEGTYYEAAKKWAEGRMG
                     VPKAVGIIHVERIFNLQSGANAGKEIT"
     gene            complement(7635..10802)
                     /locus_tag="VBC28_001136"
     CDS             complement(7635..10802)
                     /locus_tag="VBC28_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HEO6815127.1"
                     /translation="MTKKHLKTLALALTTVSVVTYSQEVYGLEREESVKQEQTQSASE
                     DDWFEEDNERKTNVSKENSTVDETVSDLFSDGNSNNSSSKTESVVSDPKQVPKAKPEV
                     TQEASNSSNDASKVEVPKQDTASKKETLETSTWEAKDFVTRGDTLVGFSKSGINKLSQ
                     TSHLVLPSHAADGTQLTQVASFAFTPDKKTAIAEYTSRLGENGKPSRLDIDQKEIIDE
                     GEIFNAYQLTKLTIPNGYKSIGQDAFVDNKNIAEVNLPESLETISDYAFAHMSLKQVK
                     LPDNLKVIGELAFFDNQIGGKLYLPRHLIKLAERAFKSNRIQTVEFLGSKLKVIGEAS
                     FQDNNLRNVMLPDGLEKIESEAFTGNPGDEHYNNQVVLRTRTGQNPHQLATENTYVNP
                     DKSLWRATPDMDYTKWLEEDFTYQKNSVTGFSNKGLQKVRRNKNLEIPKQHNGITITE
                     IGDNAFRNVDFQSKTLRKYDLEEIKLPSTIRKIGAFAFQSNNLKSFEASEDLEEIKEG
                     AFMNNRIGTLDLKDKLIKIGDAAFHINHIYAIVLPESVQEIGRSAFRQNGALHLMFIG
                     NKVKTIGEMAFLSNKLESVNLSEQKQLKTIEVQAFSDNALSEVVLPPNLQTIREEAFK
                     RNHLKEVKGSSTLSQITFNAFDQNDGDKRFGKKVVVRTHNNSHMLADGERFIIDPDKL
                     SSTMVDLEKVLKIIEGLDYSTLRQTTQTQFREMTTAGKALLSKSNLRQGEKQKFLQEA
                     QFFLGRVDLDKAIAKAEKALVTKKATKNGHLLERSINKAVLAYNNSAIKKANVKRLEK
                     ELDLLTDLVEGKGPLAQATMVQGVYLLKTPLPLPEYYIGLNVYFDKSGKLIYALDMSD
                     TIGEGQKDAYGNPILNVDEDNEGYHTLAVATLADYEGLYIKDILNSSLDKIKAIRQIP
                     LAKYHRLGIFQAIRNAAAEADRLLPKTPKGYLNEVPNYRKKQVEKNLKPVDYKTPIFN
                     KALPNEKVDGDRAAKGHNINAETNNSVAVTPIRSEQQLHKSQSDVNLPQTSSKNNFIY
                     EILGYVSLCLLFLVTAGKKGKRARK"
     gene            complement(10865..13030)
                     /gene="nrdE"
                     /locus_tag="VBC28_001137"
     CDS             complement(10865..13030)
                     /gene="nrdE"
                     /locus_tag="VBC28_001137"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HEO6815128.1"
                     /translation="MTQSDAYLSLNAKTRFRDRTGNYHFTSDKEAVEQYMIEHVEPNT
                     MVFTSLIEKLDYLVSNNYYESDLLKQYNLEFICQIFEHAYAKKFAFLNFMGALKFYNA
                     YALKTEDNRYYLEHYEDRVVMNALFLAAGDEKAAYDLVDDMLANRFQPATPTFLNAGK
                     KRRGEYISCYLLRIEDNMESISRAISTSLQLSKRGGGVALCLTNLREFGAPIKGIKNQ
                     ATGIVPVMKLLEDSFSYANQLGQRQGAGAVYLHAHHPEVLTFLDTKRENADEKIRIKS
                     LSLGLVIPDITFELAKANKDMALFSPYDIERVYGKPMSDISITEEYETLLANADIRKT
                     FISARKLFQTIAELHFESGYPYILFEDTVNAKNPHKKEGRIVMSNLCSEIAQVNTASQ
                     FSEDLTFTKVGHDVCCNLGSINIARAMDQAADFEKLIANSIRALDRVSRTSDLDSAPS
                     IKKGNAANHAVGLGAMNLHGFLATNHIYYDSQEAIDFTDCFFYAMAYYAFKASNHLAK
                     EKGTFEGFSESSYADGSYFYQYTEQNFEPKTQRVKNLLAEYGLTLPSQEDWRKLVQSI
                     KEIGLANAHLLAVAPTGSISYLSSCTPSLQPVVSPVEVRKEGALGRVYVPAYKIDADN
                     YVYYKKGAYEVGSEAIINIAAAAQKHIDQAISLTLFMTDQATTRDLNKAYIQAFKQKC
                     ASIYYVRVRQDILEGSESYDDMLDDFTSSDLEDCQSCMI"
     gene            complement(13032..13445)
                     /gene="nrdI"
                     /locus_tag="VBC28_001138"
     CDS             complement(13032..13445)
                     /gene="nrdI"
                     /locus_tag="VBC28_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HEO6815129.1"
                     /translation="MDSTVKVVYFSSKSNNTHRFVQKLACSNQRIPSDGSSILVTEDY
                     ILIVPTYAGGGDDTKGAVPKQVVQFLNVRQNREHCQGVISSGNTNFGDTYAIAGPIIA
                     RKLNVPLLHQFELLGTQEDVTRVKELLCQFTRKDK"
     gene            complement(13446..14456)
                     /gene="nrdF"
                     /locus_tag="VBC28_001139"
     CDS             complement(13446..14456)
                     /gene="nrdF"
                     /locus_tag="VBC28_001139"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001192438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HEO6815130.1"
                     /translation="MQSYYDRSQSPLDYALSEKAFPMRSVNWNKLNDDKDLEVWNRVT
                     QNFWLPEKIPVSNDLNSWRTLDADWQQLITRTFTGLTLLDSVQATVGDIAQIKHSQTD
                     HEQVIYANFAFMVAIHARSYGTIFSTLCTSQQIEEAHEWVVDTESLQARSRILIPFYT
                     GDDPLKSKVAAAMMPGFLLYGGFYLPFYLSARGKLPNTSDIIRLILRDKVIHNYYSGY
                     KYQQKVAKLSVEKQAEMKTFVFDLLYQLIDLEKAYLYELYDGFDLAEDAIRFSIYNAG
                     KFLQNLGYDSPFTEEETRISPEVFAQLSARADENHDFFSGNGSSYIMGITEETLDEDW
                     EF"
     gene            complement(15008..15916)
                     /locus_tag="VBC28_001140"
     CDS             complement(15008..15916)
                     /locus_tag="VBC28_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEO6815131.1"
                     /translation="MKTIKDWQINICLATYNGQKYLRQQLDSIIQQGYTDWICLIRDD
                     GSTDDTVAIIKEYVNRDSRFIFINSNDDRKLGSHRSFYELVNYKKADFYVFSDQDDVW
                     KENRLERYLEEAEKFNQELPLLVYSNWTSVDEKLTVLKEHNPATVIQEQIAFNQINGM
                     VIMMNHELAKLWEYRQIGAHDSYVGTLAYAVGNVAYISDSTVLWRRQVGAESLNNYGR
                     QYGVATFWQMINTSFDRASLIFAQVSDKMSLERKLFFSRFIELKNANLMRRIYLLSKL
                     KLRRKSLKETVAMTILLLTGYGKPKA"
     gene            complement(16126..19827)
                     /locus_tag="VBC28_001141"
     CDS             complement(16126..19827)
                     /locus_tag="VBC28_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEO6815132.1"
                     /translation="MDKHHSKKAILKLTLITTSILLMHSNQVNAEEQELKNQEQSPVI
                     ANVAQQPSPSVTTNTVEKTSVTAASASNTAKEMGDTSVKNDKTEDELLEELSKNLDTS
                     NLGADLEEEYPSKPETTNNKESNVVTNASTAIAQKVPSAYEEVKPESKSSLAVLDTSK
                     ITKLQAITQRGKGNVVAIIDTGFDINHDIFRLDSPKDDKHSFKTKTEFEELKAKHNIT
                     YGKWVNDKIVFAHNYANNTETVADIAAAMKDGYGSEAKNISHGTHVAGIFVGNSKRPA
                     INGLLLEGAAPNAQVLLMRIPDKIDSDKFGEAYAKAITDAVNLGAKTINMSIGKTADS
                     LIALNDKVKLALKLASEKGVAVVVAAGNEGAFGMDYSKPLSTNPDYGTVNSPAISEDT
                     LSVASYESLKTISEVVETTIEGKLVKLPIVTSKPFDKGKAYDVVYANYGAKKDFEGKD
                     FKGKIALIERGGGLDFMTKITHATNAGVVGIVIFNDQEKRGNFLIPYRELPVGIISKV
                     DGERIKNTSSQLTFNQSFEVVDSQGGNRMLEQSSWGVTAEGAIKPDVTASGFEIYSST
                     YNNQYQTMSGTSMASPHVAGLMTMLQSHLAEKYKGMNLDSKKLLELSKNILMSSATAL
                     YSEEDKAFYSPRQQGAGVVDAEKAIQAQYYITGNDGKAKINLKRMGDKFDITVTIHKL
                     VEGVKELYYQANVATEQVNKGKFALKPQALLDTNWQKVILRDKETQVRFTIDASQFSQ
                     KLKEQMANGYFLEGFVRFKEAKDSNQELMSIPFVGFNGDFANLQALETPIYKTLSKGS
                     FYYKPNDTTHKDQLEYNESAPFESNNYTALLTQSASWGYVDYVKNGGELELAPESPKR
                     IILGTFENKVEDKTIHLLERDAANNPYFAISPNKDGNRDEITPQATFLRNVKDISAQV
                     LDQNGNVIWQSKVLPSYRKNFHNNPKQSDGHYRMDALQWSGLDKDGKVVADGFYTYRL
                     RYTPVAEGANSQESDFKVQVSTKSPNLPSRAQFDETNRTLSLAMPKESSYVPTYRLQL
                     VLSHVVKDEEYGDETSYHYFHIDQEGKVTLPKTVKIGESEVAVDPKALTLVVEDKAGN
                     FATVKLSDLLNKAVVSEKENAIVISNSFKYFDNLKKEPMFISKKEKVVNKNLEEIILV
                     KPQTTVTTQSLSKEITKSGNEKVLTSTNNNSSRVAKIISPKHNGDSVNHTLPSTSDRA
                     TNGLFVGTLALLSSLLLYLKPKKTKNNSK"
BASE COUNT         6330 a         3653 c         3202 g         6663 t
ORIGIN      
        1 tcatatgatt gattttctac tgttgaaatc attacacagt cttctaggtc taactattac
       61 tgtttgtgat caaaattttt ctgttatcag agagtataaa tccgagaaaa ctatttcact
      121 tttttacaat cattacctta ttttaagtaa ctttagtaaa actcaacatg attttttatt
      181 tcattatggc tctttaggag aactttttct tgtccaccat attcaacaat attatattat
      241 catcggtccc tggcgtagca atgttattga ccctttactt cttaagaaaa agctgactga
      301 aacacaaatc aatgctagtg aacaagacta ctttattgat agactatccc aattaccttt
      361 cttctcactt agtcaaatcc gagaattact aatagtaacc aattattgtc taactggggt
      421 tgtgaaagat aaattatcag agcccttaca ttactacacg aaagggtgga gcaactcctt
      481 tgatctagat aaaattaagc aattttctaa acagaatatg agttcttaca aatatcagta
      541 tcattttgaa aataatattc taaaagctgt taaatcaggt agtgaatttc ttttgaaaga
      601 aacagtggaa caatttagca attctatcgt acctataatc agtggagatg aattgcgatc
      661 tgaaaaaaac tactccatca tgatttatga tcgtctctca caggctacca tccaagcagg
      721 acttgatatc gaaacagctt atcgagcacg agatcgtttt ataaaagaaa acgagtcaac
      781 tataagtcta aatgaagttt taaaattacg tgatactgct atcttattct atactcaaca
      841 agttcattct ttaaaaaaac atctcgaaac ccctcattct caaactattg tcgcagtgat
      901 tcgatatctt gaaataatct aaatcgcttt attaaaacag aagaaatagc taaagaatgt
      961 cacatgagtg aatcgaagtt acgaaaacta tttaagcaag aaaaacacat cactattcaa
     1021 caatattttc taaacttaaa aatcgaagct gctaagcaat tactagatga aaataagaaa
     1081 gtagaagaag tttccaattt acttggattt tccacttctt ctaatttttc aaggacattt
     1141 aaaaaaatag tgggaattag cccactagaa tataagcaaa agcctaagac aatatagtaa
     1201 tcgaaaaata ttcattttgc ttatggtgac atcaacaatc ccaaagaaac tctttcatca
     1261 aggggacttt tttatctccc gaattatact catttaatct aataatttca actcgctata
     1321 ttagacaatc aatccattca tcacctctga taacgctaat gaaataagag aatctcccaa
     1381 ctctctataa tttacctctc taccatagtt aaataatatt ttagtaaatc ttcaacagtc
     1441 attttctatc tttgatgaag ccaccacttt aaggtctcga taaaactgga taaaagaaat
     1501 tgttttaaga aatcttcagg aatgtccacc tttgtaatat actcctcacg caatctaggg
     1561 taaacatcgt gttctaattc acttctaaag cgtaataaaa aatagggatc gtcagataat
     1621 aataacgtag cgatactatc ttgattttgt tcaaaatgtt ttaagatatg aactaaatac
     1681 tcttcaaacg taacatctct accctgctta aataagtggt gaaaaaggtc ttcacagagc
     1741 tccttgagta acacttcttt gctttcatag tgagagtaaa aagttgatct tcctacattt
     1801 gcaagtccga ttacatcttg aaccgttatt ttagaataat cattttcctg tagcaagctt
     1861 ataaaagcat tataaatcgc tacttttgtt ttctgaattt gtctatcttt taccatatag
     1921 gacactttct aagttttgtt cagtattgaa caagtgttct tttttgtttc tagcaattct
     1981 cttttatctc ctagataata gaagaaagaa tcacactaca ataccagtat aacaaaagga
     2041 gaaaaatatg acatcaaaca aaaaagtggc tatcgctttt atattaaata tcagcttttc
     2101 cgtccttgaa ttcatctttg gaagtctatt cttctcagga gctatcttag ccgatgctgt
     2161 tcatgatttt ggtgatgcaa tagctattgg catctcagct actttagaaa aaaaatctaa
     2221 gaaagatgaa gatacgatat tttcattagg ttataaacgt tttagccttt taggagctct
     2281 tatcacaagt ctaattctta taagtggatc tatcttagtc atgatagaaa atatcccaaa
     2341 attgtggcat cctactcctg ttaattatca cggcatgttt attctagctg tcatcgctat
     2401 tatcattaat ggtctagcta gttttattct ccattctggt caaagtaaac atgaggaaat
     2461 attaagcctt cattttttgg aagatattct agggtggtta gctattattg ttatatctct
     2521 tatcttaaat tggaaaccgt tgtatatcct tgatccatta ttatcagttg ccatatctac
     2581 tttcatctta tctaaagcac ttcctaaatt actatcaacc ctgaaacttt ttttagatgg
     2641 tgttccagat tccatagatt acgctgcatt acatgatgaa cttaagggac tttcgcaagt
     2701 aagatctatc aatcaactca atatctggtc aatggatggt attgataatc gagcaatcat
     2761 tcactgttgc ttaaatcaat tgatatctga aaaagattgt aaaagagcta ttcgaacgat
     2821 ttgccaacat tataagataa acgatgttac tgttgaaatc gattattctt taagagaaca
     2881 tcaaaatcat tgcaaaccat taaaaaacta acctgagtgt tagttttttc cttgacttgg
     2941 agttaactct aagtgttaag attaggtcaa tactaatgaa agaaggaaaa taaagatgac
     3001 agatacaatg atattaaatg acaatcataa aataccaact ttaggtttag gtacgtggat
     3061 gatagataat gatagggtag ctcaagttgt taaagatgct atcgaattag gctaccgcca
     3121 tattgatact gcacaagcat acaacaatga aaaaggtgtc ggtgtaggga ttaaagagtc
     3181 tggtattaca cgtgaatctt tatttgttac gactaaaatt gccgctgaac ataaagatta
     3241 tgaaagtgct acaaaatcaa ttgatcaatc actagaagct ttagggcttg attatattga
     3301 tttaatgatt attcatagtc cacagccttg gaaggaatgg cgtgaaacag ataaacattt
     3361 tgaccaagga aatttagaag cttggagagc tttagaggat gctcaaaaat ctggtaagat
     3421 gaaatccatc ggtgtctcaa actttttaga aaaagactta gaaaatatct taaaaaatgg
     3481 tcatgttaaa cctgctgtca atcaaatctt agctcacatc ggaaatacac ctttcgactt
     3541 gattgattac tgtcaaagta aaggtattca agttgaagcc tattcaccaa ttgcacatgg
     3601 acaagcattg aaatctgatg gtattcaaaa aatggctgaa aaatatgggg ttagtgttgc
     3661 tcaattatgt attcaatatt tactacaatt aaacctgatc gttttaccaa aggcttctag
     3721 taaagaacat ttacagtcta atctagactt tgactttgta attagtgacg aagatatgtc
     3781 gatattaaaa tcacttatgt ttgacgatta tggggaattt tctaacttcc ctgtttttag
     3841 tggaaaatga aaccatatgc ggatttttac agatgtttaa ttttcaaaat ttttaagttt
     3901 ttcttgactt gaagttaact ctaagagtta taatgaagtt atagaaaaga ggtaaggtta
     3961 tgaaagtagc tacatttatt gaacctggca aaatggttat aactgataca ccaaaaccag
     4021 tcattgaaca agagacagat gctgttatca aaattgttag agcctgtgtt tgtggttcag
     4081 acttgtggtg gtatcgtggt atttcaaaac gtgaaagtgg ttcttttgca ggtcatgagg
     4141 ctattggtat cgttgaggaa gttggtacta aagtaactga cgtgtcaaaa ggtgattttg
     4201 ttattgttcc ctttacacat ggctgtggcc aatgtccgtc ttgtaaggct ggatttgatg
     4261 gaaattgtac aaatcatcaa gctgcaaaaa atgtaggata ccaaggtcaa tatctacgtt
     4321 atactaatgc aaactgggca ttggttaaga tcccaggaca accttctgat tatgataatg
     4381 agactcttaa ctctctgctc accttatcag atgtaatggc aactggttat catgctgcag
     4441 caactgcaga agttaaagaa ggtgacacgg tagttgtcat gggagatggt gctgttggac
     4501 tctgtggtgt tatcgctgct aaaatgttag gtgctaaccg tatcattgca atgagtcgtc
     4561 acaaagatag gcaggaacta gcattgactt ttggtgcaac ggatattgtc gaagaacggg
     4621 gtgacgaagc cgttaaacgt gttttagatt taaccaatca agcaggtgcc gatgctgttt
     4681 tagaatgtgt tggtacagag cagtctgttg atacagctac ccaaattgct aggcctggtg
     4741 ctgtcatagg acgtgttggt atcccacaaa atccagacat gaataccaat aatttattct
     4801 ggaaaaatat cggccttaga ggtggcattg cttctgtaac aacatttgat aagtctgtcc
     4861 ttttagatgc tgttctaact cataaaatta acccaggctt agtttttaca aaatcctttg
     4921 tattagatga tattcaaaag gcttatgaag caatggataa acgtgacgct atcaaatcct
     4981 tagtgattgt tgactgaata tcgaggttaa aaatgcatat caaagaagtt agtaaaaaga
     5041 ctggcattac gtcagatacc atcaggtatt atgagagaat taatcttatt ccccctattc
     5101 atcggagtga atcaggaatt cgtgagttca cacaaaacga tattgatatt ttagaatttg
     5161 tccgctattt tagaggcgca ggtgtttccg ttgaaagtct cattgattat attcatttgg
     5221 ttgaaaaagg cgattccact atcccagcta ggctagaaat tttaaaagat gaacgcgaca
     5281 atcttgaaga taggcttcaa ggcctccaag aagcacttaa tcgtctaaat cacaaaatag
     5341 acaattatca aaataaagtt gttcctagag aacaagaact ttttgatcca acaacgaaat
     5401 gaggaaatct tatgcaagaa acaactaagt ctatctttcc taagggtgaa aaaaatccat
     5461 atggtgaatt ctttattggg caatcctatc tcgcagcatt agcaaaatct ccagatggga
     5521 atgtttcagt tggaaatgta acctttgaag caggctgtcg taataattgg catgttcatt
     5581 tggacggtta tcaaatcctt ctcgttacag aaggttcagg ttggtaccaa gaagaaggaa
     5641 aagaagcagt gtcattaaaa ccaggcgacg ttatcgttac agataaaggt gttagacatt
     5701 ggcatggtgc taaaaaagat tctgaatttg cacatattgc gattacagct ggtaagagtg
     5761 agttttatga agctgttagt gatgaagaat attcaagact aggttaaatt caagtaagga
     5821 ggaatccatt atgaaagaaa aacaaacagc cggacgtcgt caattagaag aatttgcacc
     5881 tgaatttgcc agatataatg atgatatttt atttggtgag gtttgggcaa aagaggatca
     5941 tttaacagat aaaacacgtt ctattattac tatatctgcc ttaattagcg gaggaaatct
     6001 tgaacagtta gagcatcatc ttcaatttgc caaacaaaac ggagttacta aagaagaaat
     6061 tgctgacatt atcactcacc tcgcttttta tgttggatgg ccaaaagctt ggtcagcctt
     6121 taataaagct aaggaaattt ggatataaaa taacagacat agcaagagag atactttccc
     6181 aaaagccaaa aagaaagatc aaccaattat taaaccaact attaggagaa tgatatgctt
     6241 actattcaag aaatgaaaga tacaaccttt gaattacaag aaaattttag aaggttaaat
     6301 tatcctatta aacaagttgc taaagacttg caacttaata tcagtgaagt tgaatctctt
     6361 ttaagtcttg atgttactta tcctggagat gtttgtatgc tgagagatta tctagaagat
     6421 atgttgaaaa aagagggtaa agaagtttat ccattttcta gattagcatc tcattcagca
     6481 aatcgttggt acccttatga gacaccatgg cgttattaaa gaataaagat taatccatca
     6541 tctttagaga aataaacaaa aatagtcaat agattttttt tagaaaaaag ctataaaata
     6601 agtgcctctt agagaatact ctatatcata agatttcttc tttgaaccat atcattccaa
     6661 aagaaaatta tgattgctaa caagacaatt attctaaaaa gaaactattt taacgactct
     6721 ataattactt aaggaatcat aaagaataaa gatttttatg ctatcatttc aaattgttga
     6781 aaagccagca atgatacttg ctggagtaac tttagaaaat gtaaagtcaa atcaagaagg
     6841 tatccagcaa gccattggca tttgtaaaac acaacctgat tttcgtttcg attacagtgc
     6901 cacttatcaa gtagagacaa gtgtccaagc cccaaaaggg ttagaaatta ttaggatccc
     6961 aagcgctacc tatgcagtca tttctgttaa aggtccaatg ccatcgagtc ttcaagaaac
     7021 atggcgaaaa ataatacaag gattttttca agagaataat ctaaaaccag ctaactcgcc
     7081 aaacttagaa atttattcat ctcaacatcc acaagatact gattatcaaa tggaaatttg
     7141 gcttgctatc gcagatatta ctaataactc gaaaacaaac ttaatctagt attgcttaat
     7201 ataaaaaagc cccttcttga ggctttttta tattaagtga tttctttacc tgcattagca
     7261 ccagattgta aattaaaaat acgctcgaca tgaataatac caactgcttt cggaaccccc
     7321 atgcgtcctt ctgcccattt tttcgctgct tcgtaataag taccttctgt ttgaatttca
     7381 gcagttccca caaacctgta acctaaaaga cgttcacgat caacaaaggc aatttcaatt
     7441 ttcccattat cttcaatgtt aatacgagtt tgaccatctg tattttcatt ataaataaac
     7501 gttttatcat cccagagacg catagaacgt tttgggccaa tattgggttg acctttagca
     7561 tcaaccgtcg cgaccattgc taactgacta ttaataatct ctttcatttc ttgtgtaatc
     7621 atattaattt ctccttattt tcttgctcgt tttccttttt tcccagcagt tactaggaaa
     7681 agcaaacata aactaacgta tcctagaatc tcgtatataa aattattttt agaacttgtt
     7741 tgaggtaaat ttacatcaga ctgtgactta tgtaattgct gctcggacct tattggtgtt
     7801 acagctacag aattattagt ctccgcattt atattatgac ctttagccgc tctatcaccg
     7861 tctacctttt cattaggtaa agccttatta aaaatcggcg ttttataatc aactggtttt
     7921 aaatttttct ccacttgttt tttacgataa tttgggactt catttaggta ccccttaggt
     7981 gtcttaggaa gcaatcggtc tgcttctgcc gctgcatttc gaatagcttg gaaaattcct
     8041 aatctatgat attttgccaa aggaatctgg cgtattgctt taatcttatc aagggaacta
     8101 tttaaaatat ctttaatata aagaccttca taatcagcta aagtggcaac tgccaaggta
     8161 tgataacctt cattatcctc gtcaacattt aatataggat taccatatgc atctttttgt
     8221 ccctcgccaa tagtatcact catatcaagt gcataaatca attttccaga cttgtcaaaa
     8281 taaacgttca atccgatata atattctggc aatggtaaag gcgtctttaa taaataaact
     8341 ccttgtacca ttgtagcttg cgctaatggt ccttttccct cgactaaatc tgtcagcaag
     8401 tctaactctt tttccaagcg cttaacatta gcttttttaa tagcactatt attataagct
     8461 aataccgctt tgttaatact cctctcaagc aaatgaccat tctttgttgc cttcttggtc
     8521 actaaagcct tctcagcttt agctatggct ttatccaaat caacgcgacc aaggaaaaat
     8581 tgtgcttctt gaaggaattt ttgtttttct ccttgtcgga ggttagattt tgataacaac
     8641 gctttacctg cagtagtcat ttctctaaac tgagtttgag tagtctgacg taatgtagag
     8701 taatctaaac cttcgattat ttttaaaacc ttttcaaggt ctaccattgt agaagatagc
     8761 ttatctggat caatgataaa acgctcacca tctgctaaca tatgagaatt attatgtgtc
     8821 ctaacaacca ctttcttacc aaagcgtttg tccccatcat tttgatcaaa agcattaaaa
     8881 gtaatctgag ataatgtaga tgaacccttc acttctttca aatgattcct tttgaaagcc
     8941 tcttcacgaa tagtctgtaa atttggcggt aagactactt cactaagggc attatccgaa
     9001 aaagcttgga cctcaattgt ctttaattgt ttttgctcag agagatttac actttccagt
     9061 ttattggata aaaaagccat ttcaccaatt gttttaacct tatttccgat aaacataagg
     9121 tgaagcgcac cattttgtcg aaaagctgaa cgtcctattt cttgtacaga ttctggaaga
     9181 acaatggcat aaatatgatt aatatggaaa gcagcatcac ctattttgat aagtttgtct
     9241 ttcaagtcta gagttccaat acgattattc ataaaggctc cctctttaat ctcttctaaa
     9301 tcttcacttg cttcaaagga tttcaagtta ttagattgaa aagcaaaagc acctattttc
     9361 cgaatagttg aggggagctt tatttcttcc aaatcatatt tacgtaaagt tttactttga
     9421 aaatcaacat tgcgaaaagc gttatcacca atttcagtaa tagtaatacc attgtgttgt
     9481 tttggaattt ctaagttttt attacgtctt accttttgta agcctttatt tgaaaaacct
     9541 gtaacactat ttttttgata ggtaaaatct tcctctaacc atttggtata atccatatca
     9601 ggtgttgcac gccacaatga tttgtccgga ttgacgtaag tattctcagt cgcaagttga
     9661 tgtggatttt ggcctgtcct tgtgcgcaat acaacctgat tgttgtaatg ttcatctcct
     9721 ggatttcctg taaaagcttc tgattctatt ttttcaagtc catccggaag cataacattc
     9781 ctcagattat tatcttgaaa acttgcttct cctataacct taagcttact tcccaaaaat
     9841 tcaactgttt gaatacgatt agatttgaaa gcgcgttctg ctaattttat caagtgacgt
     9901 ggcaagtaaa gcttaccacc aatctgatta tcaaaaaaag ctaattctcc aatgaccttt
     9961 aggttatctg gtaactttac ttgttttaaa gacatgtgag caaaagcata gtctgaaata
    10021 gtctcgagac tctcaggaag gttaacctca gcaatattct tattgtccac aaaagcatct
    10081 tgaccaatag acttataacc atttggaata gtaagcttag tcaactggta agcattaaat
    10141 atttctccct catcaataat ttccttctga tcaatatcta aacgactcgg tttcccattt
    10201 tctcctagcc tacttgtata ttctgcaatg gccgtctttt tatctggagt aaaagcaaag
    10261 ctagctactt gtgtcaattg agttccatct gctgcatgac ttggtaaaac caagtgtgat
    10321 gtttgagata acttattaat tccagatttt gaaaaaccta ctaaagtatc ccctctagtt
    10381 acgaaatctt ttgcctccca agttgatgtt tctagagttt ccttttttga agctgtatcc
    10441 tgttttggta cttctacttt gctagcatca ttactagaat tgcttgcttc ttgtgtaacc
    10501 tctggttttg ctttggggac ttgtttaggg tcacttacca ctgactcggt tttagaacta
    10561 gagttattac tatttccatc agaaaataaa tcactaactg tttcatcaac agtagaattc
    10621 tctttagaaa catttgtttt cctctcatta tcttcttcga accaatcatc ttctgaagct
    10681 gactgggttt gttcttgttt gaccgattcc tctctttcta atccatatac ctcctggctg
    10741 tatgtcacta ctgatactgt agtaagtgcc aaggcaagcg ttttaagatg tttttttgtc
    10801 attacaataa agcctttcta aaagccaata tatttctctt aatcttctga ttgagtatat
    10861 ttagttaaat catgcaggat tgacagtctt ctaagtccga tgaagtgaaa tcatccagca
    10921 tatcatcata actctcgcta ccttctagga tgtcctgtct cactcgtaca taataaatag
    10981 aggcacattt ttgtttaaat gcttgaatat aggctttatt taaatctcgc gtagttgctt
    11041 gatctgtcat gaaaagcgtt aacgaaatag cttgatcaat gtgtttttga gcggcagctg
    11101 caatattgat aatcgcctca gatcccactt cataagctcc ttttttgtag tagacataat
    11161 tatctgcatc aatcttataa gcaggtacat aaaccctccc cagtgctcct tccttgcgta
    11221 cttcgacagg tgatacaacc ggttgaaggc tcggagtaca agaagaaaga taactgattg
    11281 accctgtcgg agcaacagct aataaatgcg cattagctaa tcctatctct ttaatacttt
    11341 gcacaagctt tctccaatcc tcttggcttg gtagagtcag accatactca gctagtagat
    11401 ttttaacacg ctgtgttttt ggctcaaagt tttgttcggt atattgatag aaataactgc
    11461 catcagcata acttgattcc gagaagccct caaaggttcc tttttctttt gctaaatggt
    11521 tagaagcttt aaaggcataa taagccatag cgtaaaagaa gcaatctgta aaatcaatag
    11581 cctcttgaga atcgtaataa atatggttag tcgccaaaaa tccatgcaaa ttcatagcac
    11641 ctaaaccaac agcatgatta gctgcattac cttttttaat agatggagca ctatctaaat
    11701 cagaagttcg agatacacga tcaagggctc taatgctatt agcaattaat ttttcaaaat
    11761 ccgccgcttg atccatagca cgcgcaatat taatagaacc taaattacaa caaacatcat
    11821 gtccaacctt agtgaaagtc aggtcttctg aaaattgact agctgtatta acttgtgcaa
    11881 tctcagaaca taaattagac ataacaattc gtccctcttt tttgtgaggg tttttagcat
    11941 ttacagtatc ttcaaataag atataaggat aaccagattc aaaatgaagc tctgctatcg
    12001 tttgaaatag ttttcgtgca ctaataaagg ttttacgaat atccgcattg gctaataaag
    12061 tttcatattc ttctgtgata gagatatcag acattggttt accatatact cgctcaatgt
    12121 catacggtga aaacaaggcc atatctttat ttgccttagc caattcaaaa gtaatatcag
    12181 gaataacaag tcctagggaa agagatttaa ttcgaatttt ctcatcggca ttctcacgtt
    12241 tagtatctaa aaacgttaat acttcaggat ggtgagcgtg taagtaaact gcaccagctc
    12301 cttgtctttg tcctaattga ttggcgtaag aaaacgaatc ttctagtaat ttcatgacag
    12361 gtacaatacc agtagcttga tttttaatac cttttattgg agcaccaaat tcccgtaagt
    12421 tggttaagca aagtgctacc ccaccacctc gttttgataa ctgaaggctt gtactaatag
    12481 cacgagaaat agattccata ttgtcttcaa tgcgcaacaa atagcaagag atatattcac
    12541 cacgacgttt tttccctgca tttaaaaagg taggtgttgc tggttgaaag cgatttgcta
    12601 gcatgtcatc tactaggtca taggcagctt tttcatcacc agcagctaaa aacagagcat
    12661 tcattacaac acgatcttca tagtgttcca aataataacg attgtcttct gttttaagag
    12721 cataagcatt ataaaatttt aaagccccca taaaatttag aaaagcaaat ttcttagcgt
    12781 atgcatgctc aaaaatttgg caaataaact caaggttata ttgttttaga aggtccgatt
    12841 catagtagtt attagaaacc aaataatcta gcttttcaat tagtgatgtg aacaccatcg
    12901 tattaggttc aacatgttct atcatatatt gttcaacagc ctctttatcc gaagtaaaat
    12961 gataattacc tgtgcgatct ctaaagcgtg tcttcgcgtt caacgagaga tatgcatctg
    13021 attgtgtcat cttatttatc ctttctagtg aattggcaaa gtaactcctt tacccttgta
    13081 acatcttctt gtgttcccag taattcaaat tgatgaagca aaggaacatt taattttcga
    13141 gcaataatag gtccagcaat ggcataagtg tcaccaaaat tggtatttcc agaagaaata
    13201 accccttggc aatgttcccg attttgtcga acatttaaaa actgtacaac ttgttttgga
    13261 actgcgcctt tggtatcatc tcctcctcca gcgtaagtag gcacaatcaa aatataatcc
    13321 tcagtaacca atatactact accatctgat ggaatacgct ggttagaaca agccaacttt
    13381 tgaacaaaac gatgggtatt gttagatttc gatgaaaaat agaccacctt cactgtacta
    13441 tccatttaaa actcccaatc ttcatccaat gtttcttctg taatccccat aatatatgaa
    13501 gagccgtttc ctgagaagaa atcatgattt tcatctgcac gcgccgaaag ttgtgcaaaa
    13561 acctcaggag atatacgagt ctcttcttct gtaaatggag aatcatatcc taaattttgt
    13621 aaaaatttac cagcattgta aatgctaaat ctgattgcat cttcagccaa atcaaaacca
    13681 tcatatagtt cgtaaagata ggctttttct aaatcaatca actgatagag gaggtcaaag
    13741 acaaatgtct tcatttcggc ttgcttttct acagataatt tagcaacttt ctgttgatac
    13801 ttatagccac tgtaatagtt atgaattacc ttatctcgta aaattaaacg aataatatca
    13861 gacgtatttg gtaattttcc tctggctgaa aggtaaaaag gaaggtaaaa gccaccatat
    13921 aacaaaaatc ctggcatcat ggctgcagca accttagatt tcaaaggatc gtcaccagta
    13981 taaaaaggaa taagtatacg tgatcgagct tgtagacttt cagtatccac tacccattca
    14041 tgtgcttcct caatttgttg gctagtgcat aaggttgaaa aaatcgttcc ataagatcgc
    14101 gcatgaatag ctaccataaa ggcaaagtta gcataaataa cttgttcatg atctgtctga
    14161 gaatgcttaa tctgagcaat atcaccaaca gtagcctgca cagaatctaa taaggttaga
    14221 ccagtaaaag tccgagtaat taattgctgc caatcagcat ccaatgttct ccaagaatta
    14281 aggtcattag aaactgggat cttttcaggt aaccagaaat tttgagttac tctgttccat
    14341 acctctaagt ctttgtcatc gtttagctta ttccaattaa cagaacgcat aggaaaagct
    14401 ttttctgaca aggcatagtc aagtggcgat tgagaacgat catagtatga ttgcattaaa
    14461 ctagtctcct aaatattaaa attaagcgag ttttaagcac caaaaaagcc tcccaagagg
    14521 actggatttt tgttaaaatc acacaactat tggaatgttc ttcttgatgt ttttttattc
    14581 tggtaagaag ctaactcttg cttcaaaacc actgttttgt taagttacgt taaacttttt
    14641 tgtcggttac ctttattaag gatctgctta aacagtatac taacacaaag ttttgtgtct
    14701 ttcaacatat atcaccacta tatcttgtgt tttcatcata atgtaaagaa tataaaaatt
    14761 aaaaaattaa gcaagcctaa tttttataaa aaaaggcttt accttcatac ttttcctgtg
    14821 taaaacaaaa aaggagtagc gattgctact ccttttccaa aacccgaaat taatgcgagc
    14881 caaggttcta ccttgttctt taatattatt atatactaat ttcgttaagt gtcaacaact
    14941 ttttgaaaaa taaatatttt ctatattttt aactacaact caacttgatt tccttcaacc
    15001 aaaaaaatta tgcttttggt ttaccgtaac ctgttaaaag taggatagtc atagcaactg
    15061 tttctttcaa tgatttccga cggagtttta attttgataa aagataaatc cttctcatta
    15121 gattggcatt ttttaattct ataaaacgac taaagaagag tttacgctcc agggacattt
    15181 tatcggatac ttgagcaaaa ataagcgaag cacgatcaaa agaagtattt atcatctgcc
    15241 aaaacgtagc caccccatat tgtctcccgt aattgtttag cgactctgcc cctacttgtc
    15301 tacgccaaag gactgtactg tcagaaatat aagccacatt tccaacggcg taagctagag
    15361 ttccaacata agaatcatgg gcgccaattt gacgatattc ccaaagcttt gctaactcat
    15421 gattcatcat gataaccata ccatttattt ggttaaaggc aatttgttct tgaataactg
    15481 ttgctggatt atgttctttc aatactgtta atttttcatc tacagaagtc cagttcgaat
    15541 aaacaagcaa tggtagttct tggttaaatt tctctgcttc ttcaaggtaa cgttctaaac
    15601 gattttcttt ccaaacatca tcctggtctg agaaaacata aaaatctgct tttttatagt
    15661 taaccaactc gtaaaaactg cggtgacttc ctaactttct gtcatcgtta gaattaataa
    15721 atataaaacg tgaatctcta ttaacatatt ctttaataat agctactgta tcatcagtag
    15781 atccatcgtc ccttattaaa caaatccagt cagtataacc ttgttgtatt atgctatcta
    15841 attgctggcg aaggtatttt tgaccattat aggtcgctaa gcaaatattt atttgccaat
    15901 cttttatcgt tttcattgct atcaaaactc cttaaggcta gttcatctta tgaaagctta
    15961 caaagtgaac ggtatcacat actaatcgta attataatat tataatatta tatgtcatta
    16021 atcaatataa aattaatcaa aaataagagt caatacccta aaagtccagc ttaacataat
    16081 gttaaactga acttttcatc atattgccga gtagtttact tagttttatt tactattatt
    16141 tttagtcttt ttgggtttca aataaagaag taaactagat aacaatgcca aagtaccaac
    16201 aaatagacca ttcgttgctc tatctgatgt actaggtaag gtatggttaa cagaatcccc
    16261 gttatgttta ggtgatatga tcttagctac tctgctacta ttattgtttg tagaagtgag
    16321 gactttctca tttcctgatt tagttatttc tttagacaat gattgagtag taactgtagt
    16381 ttgcggctta actaatatta tttcttctag attcttgttt actacttttt cttttttaga
    16441 aataaacata ggttcttttt tcaagttatc aaaatatttg aaactgttag aaattactat
    16501 agcgttttct ttctctgata ctactgcctt attcaagaga tcagacaatt ttaccgttgc
    16561 gaaattacca gctttatctt ccacaacaag tgtcaaggcc ttagggtcta ccgcaacctc
    16621 actctctcct atcttaaccg ttttaggaag tgtcacttta ccttcttgat ctatatggaa
    16681 ataatggtaa gaagtctcat ccccatattc ttcatctttt acaacatgag ataaaactaa
    16741 ttgtaaacga tatgtaggaa cataactact ttccttaggc atggctaagc ttaatgttcg
    16801 attagtttca tcaaactgag ctcgtgaagg aagatttggt gacttagtac ttacttgtac
    16861 tttaaagtct gactcctgac tatttgctcc ttctgctact ggtgtgtaac gtaagcgata
    16921 agtataaaaa ccatctgcta caactttgcc atccttatct aaaccactcc actgaagagc
    16981 atccatacga taatgaccat cactttgctt tggattatta tggaaatttt tacgataaga
    17041 tggtaaaacc ttactttgcc aaataacatt tccattttga tctagaactt gagcagaaat
    17101 atccttaaca tttcttaaga aagttgcctg gggagtgatt tcgtccctat ttccatcttt
    17161 atttggagaa atggcaaaat atggattatt cgctgcatct ctttccaaaa gatgaattgt
    17221 tttatcctca accttattct caaaagttcc taaaataatt ctttttggac tctccggtgc
    17281 taattctaac tccccaccat ttttgacata atcaacatag ccccaagacg ctgattgtgt
    17341 taacaaggca gtatagttgt tgctttcaaa aggagctgat tcattgtact ccaattggtc
    17401 tttatgagtt gtatcatttg gtttatagta gaaactacct ttagaaagcg tcttataaat
    17461 cggtgtttca agtgcttgta agttcgcaaa atcaccatta aatcctacaa aaggaatact
    17521 cattaactcc tgattactat ccttggcttc tttaaaacgt acaaaacctt ctaagaaata
    17581 accatttgcc atctgttctt ttaatttctg actaaattga ctagcatcaa tagtaaatcg
    17641 aacttgtgtt tctttatcac gaagaattac tttctgccaa ttagtatcta gcaaggcttg
    17701 tggtttaagg gcaaatttac ctttatttac ttgttctgtt gctacattag cttgataata
    17761 caattctttg acaccttcta caagtttatg aattgtaact gtgatatcaa atttatctcc
    17821 cattcgtttg agattaattt tagctttgcc atcgtttcca gtaatataat attgagcttg
    17881 gatagctttt tcagcatcaa ctacacctgc accttgctga cgtggtgaat aaaacgcctt
    17941 atcctcttca ctatataatg ctgttgctga gctcatgagg atgtttttag acaattctag
    18001 caatttttta gaatctaaat tcatcccttt atatttctca gccaaatgac tttgaagcat
    18061 tgtcattaat cctgcaacat gtggtgaagc catacttgta ccagacattg tttggtattg
    18121 attattatag gttgaagaat aaatttcaaa gccagaagct gttacatcag gcttgattgc
    18181 tccttcagct gtcacgcccc aacttgattg ttccagcata cgattaccac cttggctatc
    18241 aactacttca aaactctggt taaatgttaa ctgacttgaa gtatttttta tacgctcgcc
    18301 atctacttta ctaataatcc ccacaggtaa ttcacggtaa ggaattagaa aatttccacg
    18361 tttttcttga tcgttaaaaa taacgatacc aacaacacct gcatttgtag catgagtgat
    18421 tttagtcata aaatcaagtc caccaccacg ctcaattaat gcaatcttac ctttaaagtc
    18481 cttaccttca aagtcttttt ttgcaccata attggcataa accacatcgt aggccttacc
    18541 tttgtcaaaa ggtttagaag tcacaatcgg caacttaact aacttacctt caatagttgt
    18601 ttcaacgacc tcactgatag ttttaagtga ttcatagcta gcaacactca aagtatcttc
    18661 agaaatagct ggactattaa ccgtaccgta gtcaggatta gttgataatg gtttgctata
    18721 atccatacca aatgcgcctt catttccggc agccacaaca actgcaacgc ccttctcaga
    18781 agctaattta agtgctaatt taactttatc attgagagca attaaagaat cagctgtttt
    18841 tccaatactc atattaatcg tttttgctcc tagattaaca gcgtctgtga ttgctttagc
    18901 atatgcttca ccaaatttgt ccgaatcaat tttatctgga atacgcatta ataagacttg
    18961 agcatttggc gctgcacctt ctaaaagaag accattgatt gctggacgtt tactattacc
    19021 tacaaaaata ccagcaacgt gtgtaccatg cgaaatattc tttgcttctg aaccataacc
    19081 atctttcata gctgctgcaa tatcagccac cgtttctgta ttgttggcgt agttatgtgc
    19141 aaaaacaatc ttatcgttaa cccatttccc ataagtgata ttatgttttg cttttaattc
    19201 ctcaaattct gtcttagttt taaagctgtg cttatcatct tttgggctat ctaaacgaaa
    19261 aatatcatgg ttaatatcaa agccagtatc aataatagct actacatttc cctttcctct
    19321 ttgggttatg gcttgtaatt ttgttatttt agatgtatca agaacagcaa gcgatgactt
    19381 gctttctggc ttcacctctt catatgctga gggaactttc tgtgctattg cagttgaagc
    19441 atttgttact acattgcttt ctttattgtt ggttgtctct ggtttagagg gatattcttc
    19501 ttcaagatca gcccccaaat tagacgtatc aaggttttta gataactctt ctaataattc
    19561 atcttctgtt ttgtcatttt ttacagatgt atcacccatt tctttcgctg tattactagc
    19621 agaagcagct gttacagatg ttttttcaac agtattagta gttaccgatg gcgatggctg
    19681 ttgagcaaca ttagcaatta caggtgattg ctcttggttt tttaattctt gctcctctgc
    19741 attcacttga ttgctatgca ttaataaaat actagttgtt ataagtgtta actttaaaat
    19801 agcctttttt gagtgatgtt tatccacttt tcctatttcc tccaaaaa
//
LOCUS       DATRMA010000034        18435 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.034, whole genome shotgun sequence.
ACCESSION   DATRMA010000034 DATRMA010000000
VERSION     DATRMA010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18435
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.034"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            20..1249
                     /locus_tag="VBC28_001142"
     CDS             20..1249
                     /locus_tag="VBC28_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEO6815133.1"
                     /translation="MKKKLVSSLLKCSLIIIVSFAGGAFASFVMNHNDNIPNGGVTKT
                     SKVNYNNITPTTKAVKKVQNSVVSVINYKQQESRSDLSDFYSHFFGNQGGNTDKGLQV
                     YGEGSGVIYKKDGKNAYVVTNNHVIDGAKQIEIQLADGSKAVGKLVGSDTYSDLAVVK
                     IPSDKVSNIAEFADSSKLNIGETAIAIGSPLGTEYANSVTQGIVSSLKRTVTMTNEEG
                     QTVSTNAIQTDAAINPGNSGGALINIEGQVIGINSSKISSTSNQTSGQSSGNSVEGMG
                     FAIPSNDVVKIINQLESNGQVERPALGISMAGLSNLPSDVISKLKIPSNVTNGIVVAS
                     IQSGMPAQGKLKKYDVITKVDDKEVVSPSDLQSLLYGHQVGDSITVTFYRGENKQTVT
                     IKLTKTSKDLAKQRANN"
     gene            1347..2120
                     /locus_tag="VBC28_001143"
     CDS             1347..2120
                     /locus_tag="VBC28_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000456088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEO6815134.1"
                     /translation="MEYLETININHIAPNPYQPRLEFNTKELEELANSIKINGLIQPI
                     IVRPSAVFGYELVAGERRLRAAKLAKLESIPAIIKSYNNDDSMQLAIVENLQRSNLSP
                     IEEAKAYSQLLQKKSMTHEELAKYMGKSRPYISNTIRLLNLPPLITSAIEEGKLSSGH
                     ARALLSLPDASQQKDWYQRILTEDISVRRLEKLLKQEKKTNHKSLQNKDVFLKHQENE
                     LAQFLGSKVKLTINKDGAGNIKIAFANQEELNRIINTLK"
     gene            2319..3680
                     /gene="dnaA"
                     /locus_tag="VBC28_001144"
     CDS             2319..3680
                     /gene="dnaA"
                     /locus_tag="VBC28_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012657571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEO6815135.1"
                     /translation="MTENEQLFWNRVLELSRSQIAPAAYEFFVLEARLLKIEHQTAVI
                     TLDNIEMKKLFWEQNLGPVILTAGFEIFNAEITANYVSNDLHLQETSFSNYQQSSNEV
                     NTLPIRKIDSNLKEKYTFANFVQGDENRWAVSASIAVADSPGTTYNPLFIWGGPGLGK
                     THLLNAIGNQVLRDNPNARVLYITAENFINEFVSHIRLDSMEELKEKFRNLDLLLIDD
                     IQSLAKKTLGGTQEEFFNTFNALHTNDKQIVLTSDRNPNQLNDLEERLVTRFSWGLPV
                     NITPPDFETRVAILTNKIQEYPYDFPQDTIEYLAGEFDSNVRELEGALKNISLVADFK
                     HAKTITVDIAAEAIRARKNDGPIVTVIPIEEIQIQVGKFYGVTVKEIKATKRTQDIVL
                     ARQVAMYLAREMTDNSLPKIGKEFGGRDHSTVLHAYNKIKNMVAQDDNLRIEIETIKN
                     KIR"
     gene            3835..4971
                     /gene="dnaN"
                     /locus_tag="VBC28_001145"
     CDS             3835..4971
                     /gene="dnaN"
                     /locus_tag="VBC28_001145"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEO6815136.1"
                     /translation="MIHFSINKNFFLHALTVTKRAISHKNAIPILSTVKIEVTRDAII
                     LTGSNGQISIENTIPASNENAGLLVTNPGSILLEAGFFINIISSLPDVTLEFTEIEQH
                     QIVLTSGKSEITLKGKDVDQYPRLQEMTTDTPLTLETKLLKSIINETAFAASQQESRP
                     ILTGVHLVISQNKYFKAVATDSHRMSQRTFQLEKSANNFDLVVPSKSLREFSAVFTDD
                     IETVEVFFSDSQMLFRSENISFYTRLLEGNYPDTDRLLTNQFETEIIFNTNALRHAME
                     RAYLISNATQNGTVRLEIQNETVSAHVNSPEVGKVNEELDTVSLKGDSLNISFNPTYL
                     IESLKAVKSETVTIRFISPVRPFTLTPGEDTEDFIQLITPVRTN"
     gene            5029..5922
                     /locus_tag="VBC28_001146"
     CDS             5029..5922
                     /locus_tag="VBC28_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000987440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEO6815137.1"
                     /translation="MMSNMTLYIIANPHAGNKNASTIVGKIQELYHTEDISVFYTEQK
                     DDEKKQVINILRSFKESDHLMIIGGDGTLSKVMTYLPQHIPCTYYPVGSGNDFARALK
                     IPNLKETLTAIQTERLKEINCFIYDKGLILNSLDLGFAAYVVWKASNSKIKNILNRYR
                     LGKITYIVIAIKSLLHSSKVQVLVEGETGQQIKLNDLYFFALANNTYFGGGITIWPKA
                     SALTAELDMVYAKGHTFLKRLSILLSLVFKRHTTSKSIKHQTFKAMTVYFPKNSLIEI
                     DGEIVELDQISLKCQKRYLYM"
     gene            5932..6129
                     /locus_tag="VBC28_001147"
     CDS             5932..6129
                     /locus_tag="VBC28_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003048966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 family protein"
                     /protein_id="HEO6815138.1"
                     /translation="MYQVGSLVEMKKPHACVIKETGKKANQWKVLRVGADIKIQCTNC
                     QHVIMMSRYDFERKLKKVLQP"
     gene            6295..6534
                     /locus_tag="VBC28_001148"
     CDS             6295..6534
                     /locus_tag="VBC28_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000369332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO6815139.1"
                     /translation="MSKWENGDTTPDLDNLIRLAEIFDVSLDEHVLAKTNEVKVERIY
                     ENKPLDLRKYNKIYWFIFRNIIRSLLIILAILTFL"
     gene            6694..7809
                     /gene="ychF"
                     /locus_tag="VBC28_001149"
     CDS             6694..7809
                     /gene="ychF"
                     /locus_tag="VBC28_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEO6815140.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDERLQKLTELITPKKTVPTTFEFTDIAGIVKGASKGEGLGNKFLANIREVDAIVH
                     VVRAFDDENVMREQGREDAFVDPIADIDTINLELILADLESINKRYARVEKMARTQKD
                     KESVAEFNVLQKIKPVLEDGKSARTIEFTEEEAKVVKGLFLLTTKPVLYVANVDEDKV
                     ADPDDIDYVNQIRAFAETENAEVVVISARAEEEISELDDEDKLEFLEAIGLTESGVDK
                     LTRAAYHLLGLGTYFTAGEKEVRAWTFKRGIKAPQAASIIHSDFERGFIRAVTMSYDD
                     LIQYGSEKAVKEAGRLREEGKEYIVQDGDIMEFRFNV"
     gene            7893..8468
                     /locus_tag="VBC28_001150"
     CDS             7893..8468
                     /locus_tag="VBC28_001150"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEO6815141.1"
                     /translation="MVKMIVGLGNPGSKYNDTKHNIGFMAVDRIVKDLDVNFTEDKNF
                     KAEIGSDFINGEKIYFIKPTTFMNNSGIAVKALLTYYNISIKDMIIIYDDLDMEVGKI
                     RFRQKGSAGGHNGIKSIIAHLGTQEFDRIKVGIGRPNGRMTVINHVLGKFDKNDEIMI
                     LNTLDKVDNAVNYYLQTNDFQKTMQKYNGLK"
     gene            8465..11962
                     /gene="mfd"
                     /locus_tag="VBC28_001151"
     CDS             8465..11962
                     /gene="mfd"
                     /locus_tag="VBC28_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEO6815142.1"
                     /translation="MNIIELFSQNKVVRTWHSGLVTNSRQLVMGFSGASKAIAIASAY
                     EKLSKKIMVVTATQTDSDKLSSDISSLIGEDNVYQFFADDVPAAEFIFSSLDKSISRL
                     SALRFLKDPEKNGVLITSISGLRLLLPNPEVFSKSQYKFEIGQECYLDKLCKNLVNLG
                     YQKVSQVFSPGEFSQRGDILDIFEMTQEYPYRLEFFGDEIDGIRQFDIDTQKSLKQLE
                     SVQISPADDIILQDADFERAKKKLEGYLVTASEVQRTYLSEVLSTTENHFKHSDIRRF
                     LSIFYEKEWGILDYIPEGTPLFVDDFQKIVDRNAKLDLEIASLLTEDLQQGKSHSSLN
                     YFSDPYKQLRQYQPATFFSNFHKGLGNLKFDKLHHFTQYGMQEFFNQFPLLVDEINRY
                     KKSGATVLLQVDSQKGLNLLQENLKEYGLDLIISDKNDIVQKESQLIVGHLSNGFYFA
                     DEKIVLITEREIYHRRVKRKIRRSNISNAERLKDYNELSVGDYVVHNVHGVGKFLGIE
                     TIEIQGIHRDYLTIQYQNADRISIPVEQIELLTKYVSADGKEPKINTLNDGRFKKAKQ
                     RVAKQVEDIADDLLKLYAERSQLQGFAFSPDDNMQNDFDNDFAYVETEDQLRSIKEIK
                     QDMEGNRPMDRLLVGDVGFGKTEVAMRAAFKAVNDHKQVVVLVPTTVLAQQHFENFKE
                     RFSNYPVTVDVLSRFRSKKEQTDTLKRLSKGQVDIIIGTHRLLSQDVVFSDLGLIVID
                     EEQRFGVKHKEKLKELKTKVDVLTLTATPIPRTLHMSMLGIRDLSVIETPPTNRYPVQ
                     TYVLETNPGLVREAIIREIDRGGQVFYVYNKVDTIDQKVSELQELVPEASIGFVHGQM
                     SEIQLENTLIDFINGDYDVLVATTIIETGVDISNVNTLFVENADHMGLSTLYQLRGRV
                     GRSNRIAYAYLMYRPDKVLTEISEKRLDAIKGFTELGSGFKIAMRDLSIRGAGNILGA
                     SQSGFIDSVGFEMYSQLLEQAIATKQGKSLIRQKGNAELALQIDAYLPAEYISDERQK
                     IEIYKRIRELETRADYEALQDELIDRFGEYPDQVAYLLEIGLLKAYLDLAFTELVERK
                     GNEISILFEKASLKYFLTQDYFEALSKTQLKARISETNGKMEVVFNIKHKKNYEIIEE
                     LLKFAECFIEIKSRKPVEE"
     gene            12253..12525
                     /locus_tag="VBC28_001152"
     CDS             12253..12525
                     /locus_tag="VBC28_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEO6815143.1"
                     /translation="MRLDKYLKVSRIIKRRPVAKEVADKGRVKVNGVLAKSSTDLKLN
                     DQVEIRFGNKLLTVKVLEMKDSTKKEDAIKMYEIINETRIETDEQA"
     gene            12512..12883
                     /locus_tag="VBC28_001153"
     CDS             12512..12883
                     /locus_tag="VBC28_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000042845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEO6815144.1"
                     /translation="MSKPNVVQLNNQYINDENLKKRYEAEELRRKNRLMGWVLIFVML
                     LFILPTYNLVKSYRTLQERRQEVVKLTKDYQTLTNRTENQKLLAKQLKNPDYVQKYAR
                     AKYYFSKTGEMIYPLPDLLPK"
     gene            12886..13020
                     /locus_tag="VBC28_001154"
     CDS             12886..13020
                     /locus_tag="VBC28_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HEO6815145.1"
                     /translation="MNNLIETIEHFLAYSDDKLEELADKNYQLRQTIVEKEKIESREK
                     "
     gene            13020..14306
                     /locus_tag="VBC28_001155"
     CDS             13020..14306
                     /locus_tag="VBC28_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEO6815146.1"
                     /translation="MKKVLTFLLCSLYFVSIPAISTEEPLTLSQNRRYALTQTVVDKE
                     MYFDAIPERPTTKIEISSFQDEALTITGETLVPNTLLSIVSLTINSNGIPVFTLSNGQ
                     FIKASREAIFNDLVSKQQSVSLDYWLKPSFVTYEAPYTNGVSEVKNNLKPYSRVHLVE
                     QAETEHGIYYKTDSGFWISVEDLSVADNRMAKVQEVLLEKYNKDKYGIYIKQLNTQTV
                     AGINIDRSMYSASIAKLATLYASQEQVKLGKLSLDSKFEYKDNVNQFPNSYDPSGSGK
                     LEKKADHKLYTVKELLEATAKESDNVATNMLGYYVNNQYDSMFQTQVDTISGMHWDMK
                     KRQISPQAAGKMMEAIYYQNGDIVNYLSKTDFDNTRIPKNIPVKVAHKIGDAYDYKHD
                     AAIVYAEQPFIMIIFTDKSSYDDITKIADDVYQVLK"
     gene            14308..15582
                     /gene="tilS"
                     /locus_tag="VBC28_001156"
     CDS             14308..15582
                     /gene="tilS"
                     /locus_tag="VBC28_001156"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEO6815147.1"
                     /translation="MYNTILKDTLSKGLFTAHQKVLIAVSGGIDSINLLQFLYQYQKE
                     LSISIGIAHINHGQRKESEKEEEYIRQWGQIHDVPVFISYFQGIFSEDRARNHRYNFF
                     SKVMREEGYTALVTAHHADDQAETVFMRILRGSRLRYLSGIKQVSAFANGQLIRPFLP
                     YKKELLPNIFHFEDASNASSDYLRNRIRNVYFPALERENNQLKDSLITLSEETECLFT
                     ALTDLTRSIEVTNCYDFLRQTHSVQEFLLQDYISKFPDLQVSKEQFRVILKLIRTKAN
                     IDYTIKSGYFLHKDYESFHITKIHPKTDSFKVEKRLELHNIQIFSQYLFSYGKFISQA
                     DITIPIYDTSPIILRRRKEGDRIFLGNHTKKIRRLFIDEKITLKEREEAVIGEQNKEL
                     IFVIVAGRTYLRKPSEHDIMKGKLYIENLEKR"
     gene            15587..16129
                     /gene="hpt"
                     /locus_tag="VBC28_001157"
     CDS             15587..16129
                     /gene="hpt"
                     /locus_tag="VBC28_001157"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012961310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEO6815148.1"
                     /translation="MLENDIKKVLYSEEDIILKTKELGAKLTADYAGKNPLLVGVLKG
                     SVPFMAELLKHIDTHVEIDFMVVSSYHGGTTSSGEVKILKDVDTNIEGRDVIFIEDII
                     DTGRTLKYLRDMFKYRQANSVKVATLFDKPEGRLVDIDADYVCYDIPNEFIVGFGLDY
                     AENYRNLPYVGVLKEEIYSK"
     gene            16152..18128
                     /gene="ftsH"
                     /locus_tag="VBC28_001158"
     CDS             16152..18128
                     /gene="ftsH"
                     /locus_tag="VBC28_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEO6815149.1"
                     /translation="MKNNKNNGFLKNSFIYILLIIAVITTFQYYLKGTSSQNQQISYT
                     KLVKQLKAGEIKSISYQPSGGVVEVSGTYKKAKTIKSANSFTFLGGSVATKVTGFNSV
                     ILPNDSSIKSLVSAAEENNTNIQVKHESSSGTWISYIASFLPLVIMIGFFMMMMNQGG
                     GGGARGAMSFGKNKARSSSKDEVKVRFSDVAGAEEEKQELIEVVDFLKDPKRYKSLGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFFSISGSDFVEMFVGVGASRVRSLFEDA
                     KKAERAIIFIDEIDAVGRRRGAGMGGGNDEREQTLNQLLIEMDGFEGNESIIVIAATN
                     RSDVLDPALLRPGRFDRKVLVGQPDVKGREAILRVHAKNKPLADNVDLKVVAQQTPGF
                     VGADLENVLNEAALVAARRNKKVIDASDIDEAEDRVIAGPSKKDRTISERERAMVAYH
                     EAGHTIVGLILSNARVVHKVTIVPRGRAGGYMIALPKEDQMLLSKDDMKEQLAGLMGG
                     RVAEEIIFNAQTTGASNDFEQATAMARAMVTEYGMSEKLGPVQYEGNHAMMAGQMSPE
                     KSYSAQTAQLIDDEVRHLLNEARNKAADIINENRDTHKLIAEALLKYETLDAAQIKSI
                     FETGKMPETENDEDKARALSYDEIKEKMQEEDKK"
BASE COUNT         6558 a         2732 c         3416 g         5729 t
ORIGIN      
        1 tttacggagg tagctatttg tgaaaaaaaa attagtctca tcacttctaa agtgttctct
       61 aatcattatt gttagctttg ctggtggagc atttgctagt tttgtcatga atcataatga
      121 caatattcca aatggtggtg tcactaaaac tagtaaagta aattataata acataacgcc
      181 tacaacaaaa gctgttaaaa aggtacaaaa tagtgttgtt tctgttatca attataaaca
      241 acaagagagt cgttctgacc tatcagactt ctatagtcat ttttttggta atcagggggg
      301 caacactgat aagggcttac aagtttacgg tgaaggctct ggagtcatct ataaaaaaga
      361 tggtaaaaat gcctatgttg tcactaataa ccacgtcatt gatggggcta aacaaattga
      421 aattcaacta gctgatggct caaaagcagt tgggaaactt gttgggtcag atacctactc
      481 tgatttagcc gtcgtcaaaa ttccatcaga taaggtttca aatattgcag aatttgctga
      541 ttcatcaaaa ctcaacattg gtgaaactgc tatagcgatc ggaagccctc ttggaactga
      601 gtatgcaaat tctgtaactc aaggtattgt atctagttta aaaagaactg taacaatgac
      661 taatgaagaa ggacaaacag tttctacaaa tgctatccag actgatgctg ctatcaatcc
      721 tggtaattca ggtggagcac ttatcaatat tgaaggacag gttattggaa ttaattctag
      781 taaaatttct tctacatcaa atcaaacctc aggacaatcg tcaggaaata gcgttgaagg
      841 tatgggattt gccattcctt caaatgatgt tgttaagatt atcaatcaac ttgagagtaa
      901 cggacaagta gagagacctg ctctaggtat ttctatggct ggattaagta atttaccatc
      961 cgatgttatt agtaaactga aaatcccaag taatgttact aatggtattg tagtagcatc
     1021 tatccaatct ggcatgccag ctcaaggcaa actaaagaaa tacgatgtca ttactaaagt
     1081 tgacgataaa gaagtagtat ctccaagtga tttacaaagt ttactctatg gccaccaggt
     1141 aggggattcc ataacagtaa ccttttatcg tggtgaaaat aaacaaacag tcactataaa
     1201 acttactaaa actagtaaag atttagctaa acaacgagca aataactaaa tcaattctag
     1261 ttattttaac taagatatag taaaattaaa ggggttggtc agatagacca atccctttag
     1321 taagtctata gaatctgagg tctaaaatgg aatatttaga aacgatcaac atcaatcata
     1381 ttgctccaaa tccgtatcaa cctcgcctag aatttaatac aaaagaacta gaagaattag
     1441 ccaactctat taaaattaat ggccttatcc aacccattat tgtaagaccg tcagcagtct
     1501 ttggttacga actggtagcc ggagaacgac ggctaagagc cgcaaagtta gctaaattgg
     1561 aaagtatacc tgcaattata aaatcctata ataatgatga tagtatgcaa ttagctattg
     1621 tcgaaaattt acaacgatct aatttgtcac ctattgaaga ggctaaagcc tactctcaac
     1681 ttctacaaaa gaaaagtatg acacatgaag agttagcaaa atatatggga aaatctcgtc
     1741 cctatatatc aaatacgata cgactactaa atttaccgcc actaattact tcagctattg
     1801 aagaaggaaa actttcctct ggtcatgcgc gtgcactact cagtctacca gatgctagtc
     1861 aacagaaaga ttggtatcag agaatcctca ctgaagatat tagtgtaaga cgactagaga
     1921 agttattaaa acaagaaaag aaaacaaatc ataagtcact tcaaaataaa gatgtctttc
     1981 taaaacatca agaaaatgag ttagctcaat ttctaggtag caaagtgaaa cttactatta
     2041 ataaagatgg tgctggcaat ataaagattg ctttcgctaa ccaagaagaa ttaaacagaa
     2101 ttatcaacac tctaaaataa gggtgttgat tattttttta tttaatcaaa cttatccaca
     2161 aggtattttg ctatttttct gttgattctc taatttccac agtctgtgga aaactttaat
     2221 taacattgtt gattttattc ttcaacatct gtggaaaact ttattttttt atggtacaat
     2281 ataacaataa ttatccacaa gacaataagg aagaagctat gacggaaaac gaacaactat
     2341 tttggaatag agtactagag ctatctcgtt ctcaaatagc accagcagct tatgaatttt
     2401 ttgttctaga ggctagactc ctcaaaattg aacatcaaac tgcagttatt actttagata
     2461 acattgaaat gaaaaagcta ttctgggaac aaaatttggg gcctgttatc ctaacagctg
     2521 gttttgaaat tttcaatgct gaaattacag ctaactatgt ctcaaacgat ttacatttac
     2581 aagaaactag tttttctaac taccagcaat ctagcaatga agtaaatact ttaccaatta
     2641 gaaaaatcga ctctaacctt aaagagaaat atacttttgc taattttgtt caaggagatg
     2701 aaaatagatg ggctgtttca gcatcaattg ctgtagctga tagtcctggc acgacttata
     2761 atcctctatt tatctggggg ggacctggtc taggaaagac gcatctacta aatgctattg
     2821 gaaatcaagt cttaagagat aatccaaacg cgagggtttt atacatcact gctgagaatt
     2881 ttattaatga atttgtcagt catattcgtt tagattcgat ggaagaatta aaagaaaagt
     2941 ttcgcaactt agacttactc ctgattgatg atattcagtc gcttgctaag aaaaccttag
     3001 gggggaccca agaggagttc ttcaatactt tcaatgcttt acatacaaac gataaacaaa
     3061 tcgtattgac cagtgaccga aatccaaatc aattaaatga tctagaagaa cgtctagtca
     3121 cgcgctttag ttggggactc ccagtaaata tcacaccacc tgattttgaa acacgagttg
     3181 ctattttaac caataaaatt caagaatatc cttatgattt tcctcaagat accattgaat
     3241 acttagcagg agaatttgat tccaacgtac gtgaattaga aggagccttg aaaaatatta
     3301 gtctagttgc tgactttaag catgctaaaa ctattacagt agatatagct gcagaagcta
     3361 tcagagcacg taaaaatgat ggtcctattg ttactgtcat tcctatagaa gaaattcaaa
     3421 tacaagttgg taaattctat ggcgtaactg taaaagagat aaaagcaact aaaagaacac
     3481 aagatattgt ccttgcaaga caggtagcca tgtacttagc tcgtgagatg acagataaca
     3541 gtctcccaaa aataggtaaa gaatttgggg gacgagatca ctcaactgtt ctccacgctt
     3601 ataataaaat aaaaaatatg gttgctcaag atgacaactt acgaattgag atagaaacta
     3661 tcaaaaataa aatcagatag gctttgtgga taagtatcag aaaaatacga gacttttcca
     3721 caggttgtga acaagttatt ttccttatga atattaaagt tataatactt attcacaact
     3781 ttaacaggac ctactattac tactaaattt atactaatat ataaaggata tactatgatt
     3841 catttttcaa taaataaaaa tttcttcttg catgctctaa cggtaaccaa acgagctatt
     3901 agtcataaaa atgcgattcc aatcctttca actgttaaaa tagaagtgac tagggatgct
     3961 atcattttaa cggggtcaaa tggacaaatt tcaattgaaa atactattcc tgcttcaaat
     4021 gaaaatgcag gtttactagt aacgaatcca ggctctattt tgttagaagc tggtttcttt
     4081 attaatatta tttcaagttt accagatgta actttagaat ttacagagat tgaacaacat
     4141 caaattgttc ttactagtgg aaaatcagag attactttga aaggtaagga tgtcgatcaa
     4201 taccctcgtc tgcaggaaat gacaacagat actccattaa cattagaaac taaactgtta
     4261 aaatcaatta ttaatgaaac tgcttttgct gctagccaac aagaaagccg tccaatctta
     4321 acaggtgttc atttggttat cagtcaaaat aaatacttta aggctgttgc gacagattca
     4381 caccgtatga gtcaacgcac tttccaatta gagaaatcgg ctaataattt tgatttggtt
     4441 gttccaagta aatcccttcg agaattttcg gctgttttta cagatgatat tgaaactgta
     4501 gaggttttct tctcagatag tcaaatgtta tttagaagtg aaaatatcag cttctataca
     4561 cgtttgcttg aaggaaacta ccctgatact gatcgcctcc taactaatca gtttgaaacc
     4621 gaaattatct ttaatacaaa tgctttacgc catgctatgg aacgtgctta tttaatttcg
     4681 aatgcaactc agaacggtac tgttcgttta gaaattcaaa atgagacagt ctcagctcat
     4741 gtaaactctc cagaagttgg taaagttaat gaggaattgg atactgttag tcttaaaggt
     4801 gatagtttaa atattagctt taatccaact tacctaattg aatctttaaa agcagtaaaa
     4861 agcgaaacag ttacgattcg atttatttct ccagtacgtc catttacttt gacacctggt
     4921 gaagatactg aagatttcat ccaattaata actcctgttc gtactaacta aataagaaaa
     4981 ctacccaagt tattcttggg tagttttctt attctaagat atgctataat gatgagtaat
     5041 atgactctat atataatagc taacccccat gctggtaata aaaatgcctc cactattgtt
     5101 ggtaaaattc aggagcttta tcatactgaa gatatttctg tgttctatac agaacagaaa
     5161 gatgatgaaa aaaaacaagt cattaatata ctaaggtctt ttaaagaaag tgatcatcta
     5221 atgattatag gaggagatgg taccttatca aaagtaatga cttatctccc ccaacatatt
     5281 ccgtgcactt attatcctgt tggttcggga aatgattttg ccagagcttt gaaaattcct
     5341 aacctcaaag aaacccttac cgctattcag acagaaagat taaaggaaat taattgcttt
     5401 atttatgata aagggctgat tttgaatagt ttagatttag gttttgcagc ctatgtcgtt
     5461 tggaaagcat ctaactctaa aataaaaaat attttgaata gatatcgtct aggaaaaata
     5521 acttacattg ttatagctat caaatcatta ttacattctt ctaaagtaca agtattagtg
     5581 gaaggagaga caggacagca gataaaacta aatgaccttt attttttcgc attagcaaat
     5641 aacacttatt ttggaggtgg cattactatt tggcctaaag caagtgcttt aactgcagag
     5701 ttagatatgg tttacgctaa agggcatact tttctaaaac gattaagtat tttattatct
     5761 ttggttttta aaaggcatac tacctctaaa agtataaaac accagacttt caaagctatg
     5821 accgtatatt ttcctaaaaa tagtttgatt gaaatagatg gcgaaatagt tgagttagat
     5881 cagattagtt taaaatgtca aaaaaggtat ttatatatgt aaggagaaaa gatgtatcaa
     5941 gttggatcac ttgttgaaat gaaaaaacct catgcttgtg taattaaaga aactggtaaa
     6001 aaggctaatc aatggaaggt actgagagta ggagctgata ttaaaattca gtgtactaac
     6061 tgtcagcatg tgattatgat gagtcgttat gattttgagc gaaaattaaa aaaagtactg
     6121 caaccatgaa agtcctttta aacaaataat tatagctata tttgcaacca atactttcct
     6181 aaataatgct agtatgccgt tggaaaatta gctattctaa tgttatcgaa agaagaaagg
     6241 tggtcattga caatgacgaa agttgcagaa cagttattta tttttcggta agtcatatca
     6301 aaatgggaaa atggggatac cacaccagat ttggataatt taatcaggtt agctgagatt
     6361 tttgacgtga gcttagatga acatgtttta gctaaaacaa atgaagttaa agttgaacgt
     6421 atttatgaaa ataaacccct tgatctacga aaatacaata aaatttattg gtttattttt
     6481 cgaaatatta ttcgatctct actaattatt ttagctatat taactttctt ataagctcta
     6541 ggtattccct ttgtttcgga gtggataatt taaatgaagg tgaaagagtg gcataatgat
     6601 aagcaaatct gaaattttct tcttatcata ccgctttttc ttctattttt tgatataata
     6661 gtcttgattg aaatttgaat ggagactcac taaatggctt taacagcagg tattgtaggc
     6721 ttaccaaatg ttggtaaatc aactttattt aacgcaatta caaaagcagg agcagaagct
     6781 gctaattatc cttttgcgac tattgatccc aatgttggta tggtagaagt accagatgag
     6841 cgtctacaaa aattgacaga attgattacg cctaaaaaaa cagttccgac aacctttgaa
     6901 tttactgata ttgcaggtat tgttaaaggt gcttcgaagg gggaaggtct aggtaataaa
     6961 tttttagcta atattcgtga agtggatgct attgttcatg tggtacgtgc gtttgatgat
     7021 gaaaatgtta tgcgtgaaca aggtcgtgag gatgctttcg ttgatcctat agcagatatt
     7081 gatactatta atcttgaatt aatcttagct gatttagagt caatcaataa acgttatgcg
     7141 cgtgttgaaa aaatggcacg aactcaaaaa gataaagaat cagtagcaga gttcaatgtt
     7201 ttacaaaaga ttaaacctgt tttggaagat gggaaatcag ctcgtaccat tgagtttaca
     7261 gaagaggaag caaaagttgt taaaggtctt tttttattaa caactaaacc tgttttatat
     7321 gttgctaatg ttgatgagga caaagtagct gatcctgatg atatcgacta tgttaatcaa
     7381 attcgtgcat ttgcagaaac agagaatgca gaagtagtcg tgatttctgc tcgagctgaa
     7441 gaagaaatat ctgaacttga tgatgaagat aagttagaat ttttagaagc tattggatta
     7501 acagaatctg gtgttgataa attaacacga gctgcttatc atcttttagg ccttggaact
     7561 tactttactg ctggtgaaaa agaggttcgc gcttggacgt ttaagcgtgg tataaaagct
     7621 ccacaagctg ctagtattat ccattcagat tttgaaagag gttttattcg tgcagtaacg
     7681 atgtcatatg atgaccttat tcaatatgga tctgaaaaag cagtaaaaga agctggccgg
     7741 ttacgtgaag aaggaaaaga gtatattgtt caagatggcg atatcatgga attccgtttc
     7801 aatgtctagt aataaataat cgatttgtta acttttaggt taaggatatt gttaatatcc
     7861 ttaaccattt ttgcgtttta atggagaaga taatggtaaa aatgattgtt ggattaggaa
     7921 atccaggaag taaatataat gatacaaaac ataatattgg atttatggct gttgacagaa
     7981 ttgtaaaaga ccttgacgtc aactttacag aagataaaaa ttttaaagca gagataggta
     8041 gtgattttat caacggtgaa aaaatttatt ttatcaaacc gactactttt atgaataaca
     8101 gtggtattgc tgtaaaagcc ctgcttactt actacaacat ttctattaag gatatgatta
     8161 tcatatatga tgatttagac atggaagtcg gtaaaatacg ttttcgtcaa aaaggatctg
     8221 ctgggggaca taatggtatc aagtctatta ttgctcattt aggtactcaa gaatttgatc
     8281 gtattaaagt aggtatagga cgtccgaatg gacgtatgac agttattaat catgttctag
     8341 ggaaatttga taaaaatgat gaaattatga tattaaatac ccttgataag gttgacaatg
     8401 ctgtcaatta ttatttacag acgaatgatt ttcaaaaaac aatgcagaaa tacaacggtt
     8461 taaaatgaat attatagaat tatttagcca aaataaagtg gtgcggacat ggcactctgg
     8521 attagtaact aatagtcgcc aattagttat ggggttttca ggagcaagta aagctattgc
     8581 catagcatca gcctacgaga agttatctaa aaaaattatg gtagtcactg ccactcaaac
     8641 cgattctgat aaattatcaa gtgatatatc aagtttaatt ggagaagata acgtttatca
     8701 attttttgct gatgacgttc ctgcagcaga gtttattttc tcttcattag ataaatcaat
     8761 ttcacgactc agtgccctga ggtttctaaa agatccagaa aaaaacggtg ttttgattac
     8821 tagtatcagt ggtttaaggt tactattacc aaatccggaa gttttctcga aaagtcaata
     8881 taagtttgaa atcggtcaag aatgttattt agataaattg tgtaagaacc tagtcaattt
     8941 aggttatcag aaagtttctc aagtatttag tcctggtgaa tttagccaaa gaggagatat
     9001 tttagatatt tttgagatga cacaagaata tccttatcgc ttggaattct tcggagatga
     9061 aatagatggc atcaggcagt ttgatattga cactcaaaag tctcttaaac aattagagtc
     9121 agtacagatt tcgccagcag atgatattat tttacaagat gcagatttcg agcgtgcaaa
     9181 gaaaaaactt gagggctatc tagtaacagc tagtgaggta caacgcacct atttgagcga
     9241 ggttttatct acaactgaga atcattttaa gcactcagat attcgtcgtt ttctaagtat
     9301 cttttatgaa aaagaatggg gaatattaga ctatattcct gagggaactc cactttttgt
     9361 tgatgatttt caaaaaattg tagatcgaaa tgctaagtta gatttagaaa tagctagcct
     9421 tctgacggaa gatttacaac aaggcaagtc tcattctagt ttaaactatt tttcagatcc
     9481 ttataagcaa ttaagacaat accaaccagc aaccttcttt tcaaatttcc acaaaggatt
     9541 aggaaatctg aaatttgata aacttcatca ttttactcaa tatggaatgc aagaattttt
     9601 taatcaattt ccattgttgg tcgatgaaat caatcgttat aagaaatctg gagcgacagt
     9661 actgttgcaa gtagattctc aaaaaggttt aaatctcctt caagaaaatt taaaagaata
     9721 tgggttagat ttaatcattt cagataaaaa cgatatcgtt caaaaggagt cacaacttat
     9781 tgttgggcat ctttcaaatg gtttttattt tgcagatgag aaaattgttc tgattactga
     9841 acgtgagatt tatcataggc gtgttaaaag gaaaattaga cgcagtaata ttagtaatgc
     9901 agaacgtcta aaagattata atgaactttc agttggcgat tatgttgttc ataatgttca
     9961 tggcgtagga aaatttttag gtattgaaac aattgagata caaggtattc atcgtgatta
    10021 tctgactatt caatatcaaa atgctgatcg tatctctatc cccgtagaac aaattgaact
    10081 tctaacaaaa tatgtctctg cagatggcaa agagcctaaa attaatacgt taaatgatgg
    10141 tcgcttcaaa aaagcaaaac agagagttgc aaagcaagta gaagatattg ctgatgattt
    10201 gttgaaactt tatgcagagc gtagtcaatt acaaggattt gcattttctc ctgacgataa
    10261 tatgcaaaat gactttgata atgattttgc atatgtagag acagaggatc aattgcggtc
    10321 tataaaagaa attaaacagg atatggaagg taatcgtccg atggatcgat tattggttgg
    10381 agacgttggt tttggtaaga cagaggttgc tatgcgtgcg gctttcaaag ctgtcaatga
    10441 ccataaacaa gttgttgttt tagtcccaac aacagttcta gcccagcagc attttgaaaa
    10501 ttttaaagaa cgttttagca attatcctgt tacagttgat gtacttagtc gttttcgtag
    10561 caaaaaagaa caaacagata cactaaaaag attatctaag ggccaagttg atattattat
    10621 tggaacacat cgacttctct ctcaagatgt tgtattttcg gatttagggt taatagttat
    10681 tgatgaggaa cagcgatttg gtgtaaagca taaagaaaag ttaaaagaat taaaaacaaa
    10741 agttgatgta ttaactttga cagcgacccc aatacctaga acattacata tgtcaatgct
    10801 tggcattaga gacttatcag tcattgaaac acctccaact aatcgttatc ctgtgcagac
    10861 ttacgttttg gagacaaatc ctggtttagt tagagaagct attattcgtg agatagacag
    10921 aggaggacag gttttttatg tttacaataa agttgacact attgatcaga aagtttcaga
    10981 acttcaggag ttagtaccag aagctagtat tggttttgtt catggtcaaa tgtctgaaat
    11041 tcaattagaa aatacattaa tagactttat caatggagat tacgatgttt tagttgcaac
    11101 gacaataatt gagacaggag tcgatatttc aaatgttaat accctctttg ttgaaaatgc
    11161 agatcatatg ggcttgtcaa ccctttatca attaagaggc cgtgtgggta gaagtaatcg
    11221 cattgcttat gcttacctta tgtacagacc tgataaagtt ttaacagaaa tttctgagaa
    11281 aagactagat gcaatcaaag ggtttacaga attgggatct ggttttaaaa tagcgatgcg
    11341 tgatttatct atacgtggtg caggaaatat tttaggagct tcacaaagtg gttttattga
    11401 ttcggttgga ttcgagatgt attcacagct tctagagcaa gcgattgcca ccaaacaagg
    11461 gaaatcatta attcgtcaaa aaggtaatgc tgaattagcc ttacaaattg acgcctattt
    11521 accagcagaa tatatatcag atgagcgcca aaaaatagaa atatataagc gtattcgtga
    11581 attagaaact agggctgatt atgaagcctt acaagatgag ttaatagatc gttttggaga
    11641 atatcctgat caagttgcat accttttaga aataggactt ttaaaagctt atctggacct
    11701 tgcctttact gaattagtag aaagaaaagg taatgaaata agcatcctat ttgaaaaagc
    11761 atctttgaag tattttttaa ctcaagatta ttttgaagca ctatcaaaaa ctcaacttaa
    11821 ggctagaatt agcgaaacaa atggtaagat ggaagttgtt tttaatatca aacataagaa
    11881 aaattatgaa attattgaag aacttctaaa atttgccgaa tgttttatcg agattaagtc
    11941 acgtaaacca gtagaagaat aactaagtga aaagttgcaa ggaaatttgg atactctttt
    12001 agaaaatgtt tggttcaaga agaatagtac gagcaacatt atgtttcacg tcaaacatct
    12061 caacttttga aagacataaa tatttttaga ggttattaaa atagaagttt tttacttgat
    12121 atttgagaga attaagagag ctattagctc tctttttgtt acattatatt gacaaatatt
    12181 atcagttcag atatttttca aaaaaaatgc taaaatagta gagttaattt agatagaagg
    12241 atgtgatcat cagtgagatt agacaagtat ctaaaagtgt cgcgtatcat taagcgtcgt
    12301 cccgttgcta aagaagttgc agacaaagga cgtgtcaagg tgaatggcgt attagcaaag
    12361 tcatcaactg acctaaagct taatgaccaa gttgaaattc gttttggtaa taaattgttg
    12421 accgttaaag tattagaaat gaaagatagc actaagaaag aagatgctat caaaatgtat
    12481 gaaattatta atgaaacaag gatagaaaca gatgagcaag cctaatgttg ttcagttaaa
    12541 taatcaatat attaacgatg agaatctaaa aaaacgttac gaagctgagg agttacgccg
    12601 aaaaaatcgt ttaatgggtt gggttcttat ttttgtcatg cttttattta ttttacccac
    12661 ttataattta gttaagagtt acagaacttt acaagaacgt cgtcaagaag ttgtaaaatt
    12721 aacgaaagac tatcagacat taactaatag aactgagaac cagaagttgc tagcaaaaca
    12781 actaaaaaat ccagattacg ttcaaaaata tgctcgagct aagtattatt tctctaagac
    12841 cggcgaaatg atttacccat taccagacct tttaccaaaa taattatgaa taatttaatt
    12901 gaaacaatag aacattttct agcctactcg gatgacaaac tagaagaatt agctgataag
    12961 aactatcaac ttaggcaaac aattgtagaa aaagaaaaga ttgagagcag agaaaagtaa
    13021 tgaagaaagt tttaaccttc ttattatgta gcttatattt tgtatcaatc ccagctataa
    13081 gtaccgagga gccattaacg ctttctcaaa atagacgata cgccttaact cagacagttg
    13141 tggataagga aatgtatttt gatgctattc cagagagacc aactacaaaa attgaaataa
    13201 gcagttttca agatgaagct ttaaccataa caggagagac tttagttcca aatactttat
    13261 tgtcgattgt ttctcttaca attaattcaa atggaattcc agtatttact ttgtccaatg
    13321 gacagttcat aaaggctagt cgtgaagcta tttttaatga tttagtttct aagcagcaat
    13381 ctgtctcatt ggattattgg ttaaaacctt cttttgtgac ttatgaagca ccttacacaa
    13441 atggtgttag tgaagttaaa aataatttaa aaccttattc cagagtgcat ttagtagaac
    13501 aagcagaaac agaacatggt atttattata aaacggacag tggtttttgg atatcagtag
    13561 aagatctatc ggttgctgat aatcgtatgg ctaaggttca agaagtatta ttggaaaaat
    13621 ataataaaga taaatatggt atatacatca aacaattaaa tacacagaca gtagctggta
    13681 ttaatattga taggtcaatg tactctgcaa gtattgcaaa attagcaaca ctttatgcta
    13741 gccaagagca agtaaaatta ggaaaactgt cattggacag caaatttgaa tacaaagata
    13801 atgttaatca atttcctaat agctatgatc caagtggtag tgggaaatta gagaaaaaag
    13861 ctgatcataa attatatacc gttaaggagt tacttgaagc aactgccaag gaatcagata
    13921 atgttgcaac aaatatgcta ggttattatg tcaataatca atatgattca atgtttcaaa
    13981 cacaagtaga cacaatttct ggtatgcact gggatatgaa aaaacgccag atttctccac
    14041 aagctgcggg taaaatgatg gaagcaattt attatcaaaa tggagatatt gttaattacc
    14101 tatcaaagac tgattttgat aatacaagaa tccctaaaaa tataccagtt aaagtagcac
    14161 ataaaattgg agatgcttat gactacaaac atgatgcggc tattgtttat gcagaacaac
    14221 cttttattat gattatcttt acggataaat catcttacga tgatatcact aagattgctg
    14281 acgacgttta ccaggtttta aaataatatg tataacacta ttttgaaaga taccttatcc
    14341 aaagggttat ttaccgctca tcagaaagtt ctaattgctg tttctggtgg cattgattca
    14401 ataaatttgt tgcaattttt ataccagtat caaaaagagt tatcgattag tattggtatt
    14461 gcacacataa atcatggaca gcgaaaagaa tcagaaaaag aagaagaata tatcagacag
    14521 tggggacaga tacatgatgt ccctgtcttt atctcttatt ttcaaggaat attttcagaa
    14581 gatagagcac gtaaccatcg ctacaatttt ttttcaaaag ttatgcgtga agaaggttac
    14641 acggctttag taacagctca ccatgctgat gatcaagctg aaactgtatt tatgagaatt
    14701 ttaagaggaa gcaggctacg ctatttatca gggattaagc aagtgagtgc ttttgcaaac
    14761 ggtcaattga ttagaccatt tcttccatat aaaaaggagt tattaccaaa tatttttcat
    14821 tttgaagatg ctagcaacgc ttcgtctgat tatttaagaa atcgaataag aaacgtctac
    14881 tttcctgctt tggaaagaga aaataatcaa ttgaaagatt ctctcataac tctctctgag
    14941 gaaacagagt gtttattcac ggctttaaca gacctaacaa ggagcatcga agtaacaaac
    15001 tgctatgatt ttttaaggca aactcattct gtccaagaat ttttattgca agattacatt
    15061 agcaaatttc cagatttaca agtgagtaaa gaacagttca gagtaatatt aaaattgatt
    15121 agaactaaag ccaatattga ctatactatt aaatcgggct attttcttca taaggattac
    15181 gagagttttc atattacaaa aatccatcct aagacggata gttttaaggt tgaaaaaaga
    15241 ttagaattgc ataatataca aatattctca caatatttat tttcctatgg aaaattcatt
    15301 tctcaagcag acattactat tcctatttat gatacttctc cgattatttt aagaagaaga
    15361 aaagaagggg ataggatatt tttaggaaat cacactaaaa aaattcgccg tctatttatt
    15421 gatgagaaaa taactctaaa agagagagaa gaagctgtta ttggggagca aaataaagag
    15481 ttgatatttg tgattgttgc aggtagaaca tatttgagaa aaccttctga acatgatata
    15541 atgaagggca aactttatat tgaaaattta gaaaaaaggt gacatcatgc ttgaaaatga
    15601 tataaaaaaa gttttatatt cagaagaaga tattattctg aaaacgaaag aattaggagc
    15661 taaattaact gcagattatg caggtaagaa tccattatta gtaggtgtat taaaggggtc
    15721 agtgcctttt atggctgagc tattaaagca tattgataca catgttgaaa ttgattttat
    15781 ggttgtttca agctatcatg gtggtacaac tagtagtgga gaagttaaaa ttttaaaaga
    15841 tgttgatact aacattgaag gaagagatgt aatctttatt gaagacatca ttgatactgg
    15901 acgtacatta aaataccttc gagatatgtt taagtatcgc caagctaact ctgtcaaagt
    15961 tgcaaccctt tttgataagc cagaagggcg attggtagat attgatgctg attacgtttg
    16021 ttacgatatc ccaaatgaat ttattgtggg attcggttta gactatgctg aaaactatcg
    16081 taacttacct tatgttggtg tgttaaaaga agaaatttac tcaaaataga aaaaaggtac
    16141 tctcttaaac tatgaaaaat aataaaaata atggttttct gaaaaattcc tttatttaca
    16201 tattattgat tattgcggtt attacaacct ttcaatacta tttaaaagga actagctctc
    16261 aaaaccagca aatttcttat acaaaacttg ttaaacaatt aaaagctgga gaaattaagt
    16321 ctatctcata ccagccaagt ggtggtgttg ttgaagttag tgggacatat aagaaagcca
    16381 aaacaattaa atcagctaat agttttactt ttttaggtgg tagtgttgct actaaagtta
    16441 caggatttaa ttctgttata ctaccaaatg actcttcaat aaaatctctt gttagcgctg
    16501 ctgaagaaaa taacactaat attcaggtca aacatgaaag ttcaagtggg acatggattt
    16561 cttatattgc cagtttttta cctttagtta tcatgattgg tttcttcatg atgatgatga
    16621 accaaggcgg tggcggtggc gctcgtggtg ccatgagctt tggtaaaaat aaagcaagat
    16681 catctagtaa agatgaggtt aaagttcgat tttcggatgt tgctggagca gaggaagaaa
    16741 agcaagaatt aatagaagtc gttgacttcc ttaaagatcc taaacgatat aaatctctag
    16801 gtgcccgaat tccagcaggg gttctattag aaggacctcc agggacaggt aaaaccctat
    16861 tagcaaaagc tgttgccggt gaagcagggg ttccattctt tagtatttcc ggttcagatt
    16921 ttgttgaaat gtttgtcggt gttggagcta gtcgagtacg ctctttgttt gaagatgcta
    16981 aaaaagctga acgtgctatt attttcattg atgaaattga tgctgttgga cgtcgacgtg
    17041 gagctggtat gggtggcgga aacgatgaac gtgaacaaac gctcaaccaa cttttaattg
    17101 aaatggatgg ttttgaagga aacgaaagta ttatcgttat cgctgctacc aaccgtagtg
    17161 atgtgttaga tcccgcatta ctgcgtccag gacgttttga tagaaaagtt ctagttggac
    17221 aacctgatgt aaaaggtcgt gaagcaattc tgagagtaca tgctaaaaat aaaccattag
    17281 cagataatgt tgacttaaaa gtagtagctc aacaaacccc aggatttgtt ggtgctgatt
    17341 tggagaatgt tctcaatgaa gctgctttag ttgctgcgcg acgaaataaa aaagttattg
    17401 atgcttcaga tattgatgaa gcagaagacc gtgtaattgc tggtccttct aaaaaagatc
    17461 gtactatttc tgaaagagaa agagctatgg ttgcttacca tgaagctggg cacacaattg
    17521 tcggtttgat tctttcaaat gcgcgagttg ttcataaagt tactatcgta ccacgtggtc
    17581 gtgcaggtgg atatatgatt gctttaccta aagaagatca aatgcttctt tcaaaagatg
    17641 atatgaaaga gcaattagca ggtcttatgg gaggacgtgt agcggaagaa attattttca
    17701 acgctcagac aacaggtgcc tcaaatgact tcgaacaagc cactgctatg gcccgcgcta
    17761 tggtaactga atatggtatg agcgagaaac ttggaccagt tcaatacgaa ggcaaccacg
    17821 caatgatggc tggtcaaatg tcacctgaaa aatcatattc tgctcaaact gcacaactta
    17881 ttgatgatga agttcgtcac cttcttaatg aagcacgtaa taaggcagct gatattatca
    17941 acgaaaaccg tgatactcat aaattaattg cagaagccct cttaaaatat gagactcttg
    18001 atgctgctca gattaaatcg atttttgaga caggtaaaat gcctgagaca gaaaatgatg
    18061 aagataaagc aagagcgtta tcatacgatg aaatcaaaga aaaaatgcaa gaagaagata
    18121 aaaaataagt catcatatat ttttaaagaa ctttataaat ttcaaatacc cttcaaattt
    18181 agataaaatt tgaagggtat tttttatgaa gatgaagttt aaatttgtga attaagaaga
    18241 tgaaatatga acatgacaac ggtaaaaatg ctatccattc aacaagtata gaaggttagt
    18301 tactataaat aatattaact ttttaaaaat aaaagaaaaa atgcaaaaat agtattgaca
    18361 ggaagtagaa atatttagta taatgaataa gttgtctcga gagagcagct tctagtaacg
    18421 cgtagttaac aactt
//
LOCUS       DATRMA010000035        18211 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.035, whole genome shotgun sequence.
ACCESSION   DATRMA010000035 DATRMA010000000
VERSION     DATRMA010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18211
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.035"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            602..916
                     /locus_tag="VBC28_001159"
     CDS             602..916
                     /locus_tag="VBC28_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEO6815150.1"
                     /translation="MELKFVIPNMEKTFGNLEFAGEDKVVQRRINGRLTVLSRSYNLY
                     SDVQRADDIVVVLPAEAGEKHFGFEERVKLVNPRITAEGYKIGTRGFTNYLLHADDMI
                     KE"
     gene            932..1318
                     /locus_tag="VBC28_001160"
     CDS             932..1318
                     /locus_tag="VBC28_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEO6815151.1"
                     /translation="MMRLANGIVLDKDTTFGELKFSALRREVRIQNEDGSVSDEIKER
                     TYDLKSKGQGRMIQVSIPASVPLKEFDYNARVELINPIADTVATATYQGADVDWYIKA
                     DDIVLTKDSSSFKAQPQAKKEPTQDK"
     gene            1347..2591
                     /locus_tag="VBC28_001161"
     CDS             1347..2591
                     /locus_tag="VBC28_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEO6815152.1"
                     /translation="MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQIN
                     WQDFNLSQADKIDIPYLIISFSVAILICLLVAFVFKRVRYDTVKQLYHRQKLAKMILE
                     NKWYESEQVKTEGSFKDSAGRTKEKITYFPKMYYRLKNGLIQIRVEITLGKYQDQLLH
                     LEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRLMKNVWWEY
                     DKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGLPAHFLIFDE
                     YVAFMEMLGTKENTAVMNKLKQIVMLGRQAGFFLILACQRPDAKYLGDGIRDQFNFRV
                     ALGRMSEMGYGMMFGSDVQKDFFLKRIKGRGYVDVGTSVISEFYTPLVPKGYDFLEEI
                     KKLSNSRQSTQATCEAEVAGVD"
     gene            2594..2746
                     /locus_tag="VBC28_001162"
     CDS             2594..2746
                     /locus_tag="VBC28_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEO6815153.1"
                     /translation="MLAGVAIATPALNPPYLTGGYKSTGNSQKNIRKSFGYKGFTKFQ
                     RMSNGL"
     gene            2769..3974
                     /locus_tag="VBC28_001163"
     CDS             2769..3974
                     /locus_tag="VBC28_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEO6815154.1"
                     /translation="MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIE
                     TGKVKPSEDLQQSLWEALERFNPDAPLEMLFDYVRIRFPTTDVQQVVENILQLKLSYF
                     LHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEFF
                     MDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRSGELVRKEE
                     KECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAV
                     RDLLVYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKP
                     EPYSFQRTLNWLSHQVAPTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVK
                     EQDVITTKK"
     gene            4017..4238
                     /locus_tag="VBC28_001164"
     CDS             4017..4238
                     /locus_tag="VBC28_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6815155.1"
                     /translation="MNFGQNLYNWFLSNAQSLVLLAIVVIGLYLGFKREFSKLIGFLI
                     IAIIAVGLVFNAAGVKDILLELFNRIIGA"
     gene            4355..4852
                     /locus_tag="VBC28_001165"
     CDS             4355..4852
                     /locus_tag="VBC28_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HEO6815156.1"
                     /translation="MDDMQVYIANLGKYNEGELVGAWFTFPIDFEEVKEKIGLNDEYE
                     EYAIHDYELPFTVDEYTSIGELNRLWEMVSELPEELQSELSALLTHFSSIEELSEHQE
                     DIIIHSDCDDMYDVARYYIEETGALGEVPASLQNYIDYQAYGRDLDLSGTFISTNHGI
                     FEIVY"
     gene            4827..5333
                     /locus_tag="VBC28_001166"
     CDS             4827..5333
                     /locus_tag="VBC28_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016897981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEO6815157.1"
                     /translation="MGFLKSSIKSVGTLLLADFLFYGVAQSATPIFYERIDYMKKIRS
                     YTSIWSVEKVLYSINDFRLPFPITFTQMTWFVVSLFAVMILGNLPPLSMIEGAFLKYF
                     GIPVAFTWFMSTKTFDGKKPYGFLKSVIAYALRPKLTYAGKKVTLGRNQPQEAITAVR
                     SEFYGISN"
     gene            5317..7764
                     /locus_tag="VBC28_001167"
     CDS             5317..7764
                     /locus_tag="VBC28_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEO6815158.1"
                     /translation="MAYPIKYIENNLVWNKDGECYAYYELVPYNYSFLSPEQKIQVHD
                     SFRQLIAQNRDGKIHALQISTESSIRSAQERSKNEVTGKLKAVAYDKIDQQTDALISM
                     IGENQVNYRFFIGFKLLLNDQEFSMKSLTVEAKNALSDFVYDVNHKLMGDFVSMSNDE
                     ILRFQKMEKLLENKISRRFKIRRLDKDDFGYLIEHLYGQTGTAYEEYEYHLSKKKLDN
                     ETLIKYYDLIKPTRCLVEEKQRYLKIQQEDETVYVAYFTINSIVGELDFPSSEIFYYQ
                     QQQFTFPIDTSMNVEIVANRKALSTVRNKKKELKDLDNHAWQSDNETSSNVAEALESV
                     NELETNLDQSKESMYKLSYVVRVSANDLDELKRRCNEVKDFYDDLSVKLVRPFGDMLG
                     LHEEFLPASKRYMNDYIQYVTSDFLAGLGFGATQMLGENEGIYVGYSLDTGRNVYLKP
                     ALASQGVKGSVTNALASAFVGSLGGGKSFANNLIVYYAVLYGAQAVIVDPKAERGRWK
                     ETLPEISHEINIVTLTSDEKNKGLLDPYVIMKNPKDSESLAIDILTFLTGISSRDGER
                     FPILRKAIRAVTNSEVRGLMKVIEELRVENTPLSTSIADHIESFTDYDFAHLLFSNGY
                     VEQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVAMLIVISTFALDFIHTDRS
                     IFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFVTQNTDDLLDEKLKNNLGL
                     KFAFRSTDLNEIKKTLAFFGVDPEDENNQKRLRDLENGQCLISDLYGRVGVIQFHPVF
                     EELLHAFDTRPPVRKEV"
     gene            7767..9944
                     /locus_tag="VBC28_001168"
     CDS             7767..9944
                     /locus_tag="VBC28_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEO6815159.1"
                     /translation="MKPSIVNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVSANGFSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHAIMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNLTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRV
                     MRKPRMLMHAHMHRLQRKLGRSMTTLGAGSAIVTGKKGQSGSGSSARTQADHSRPDGK
                     EKSTLGKRIGQTIGTVADTKDRMVDTASGLKEQVKDLPTNARYAVYQGKSKVKENVRD
                     LTSSISQTKADRASGRKEQQEQRRKTIAKRRSEMEQVKQKKQPASSVHERPTTRQEQY
                     HDEQTSKQSNIQTSYKESQQAKQERPAVKSDFSSPKVERQGNTVQEKTVQKPATSTTT
                     ADRTSQRPITKERPSTVQRVPLQNTRSRPPIKTATIKKVGKKP"
     gene            9941..10942
                     /locus_tag="VBC28_001169"
     CDS             9941..10942
                     /locus_tag="VBC28_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HEO6815160.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDSGISNIHYGGV
                     NVSAEVLAHKPMVEKYAKEYGVEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLSVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGGEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKYEGWRYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTQHIPLSEAQA
                     GDLVFFHSTYNAGSYITHVGIYLGNNRMFHAGDPIGYADLTSPYWQQHLVGAGRIKQ"
     gene            10939..11871
                     /locus_tag="VBC28_001170"
     CDS             10939..11871
                     /locus_tag="VBC28_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEO6815161.1"
                     /translation="MRKEDLMMKFRKNQNKEKQIPKEKKPRVYKVNPHKKVVIALWVL
                     LGLSFSFAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWEQSDKSI
                     DNRMESLKGYLTDELQALNVDTVRKDIPVSSSVRGFQIWTVEPTGDNEFNVTYSVDQL
                     ITEGENTKTVHSAYIVSVYVDGSGNMVLVKNPTITNIPKKSSYKPKAIESEGTVDSIT
                     TNEINEFLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIHNRKDNQVTV
                     SLTVEYIDQQTKATQVSQFDLVLEKNGSNWKIVK"
     gene            12116..12232
                     /locus_tag="VBC28_001171"
     CDS             12116..12232
                     /locus_tag="VBC28_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HEO6815162.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
     gene            12248..14167
                     /gene="tet(M)"
                     /locus_tag="VBC28_001172"
     CDS             12248..14167
                     /gene="tet(M)"
                     /locus_tag="VBC28_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HEO6815163.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFHNCSLFPVYH
                     GSAKNNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            14286..14453
                     /locus_tag="VBC28_001173"
     CDS             14286..14453
                     /locus_tag="VBC28_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HEO6815164.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(14513..14866)
                     /locus_tag="VBC28_001174"
     CDS             complement(14513..14866)
                     /locus_tag="VBC28_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO6815165.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLM
                     ESLASGINEARNIED"
     gene            15371..15793
                     /locus_tag="VBC28_001175"
     CDS             15371..15793
                     /locus_tag="VBC28_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEO6815166.1"
                     /translation="MKPSSFQTTIENQFDYICKRAMEDERKNYMLYLSRIAKREVSFS
                     DVGDYLVSQFATTDNYSTDFQIFTLNGLSVGVENDLLSEALRELPDKKREILLLFYFM
                     DMSDSEIADLLKLNRSTVYRHRTSGLALIKKFMEEFEE"
     gene            15790..16020
                     /locus_tag="VBC28_001176"
     CDS             15790..16020
                     /locus_tag="VBC28_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO6815167.1"
                     /translation="MKTQYPMIPFPLIVKATDGDTEAINQILHHYRGYITKRSLRLMK
                     DEYGNQSMVVDEVLRGRMETRLITKILSFEIK"
     gene            16481..16684
                     /locus_tag="VBC28_001177"
     CDS             16481..16684
                     /locus_tag="VBC28_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006730246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HEO6815168.1"
                     /translation="MKQTDIPIWERYTLTIEEASKYFRIGENKLRRLAEENKNANWLI
                     MNGNRIQIKRKQFEKIIDTLDAI"
     gene            16766..17983
                     /locus_tag="VBC28_001178"
     CDS             16766..17983
                     /locus_tag="VBC28_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020220015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEO6815169.1"
                     /translation="MSEKRRDNKGRILKTGESQRKDGRYLYKYIDSFGEPQFVYSWKL
                     VATDRVPAGKRDCISLREKIAELQKDIHDGIDVVGKKMTLCQLYAKQNAQRPKVRKNT
                     ETGRKYLMDILKKDKLGVRSIDSIKPSDAKEWAIRMSENGYAYQTINNYKRSLKASFY
                     IAIQDDCVRKNPFDFQLKAVLDDDTVPKTVLTEEQEEKLLAFAKADKTYSKNYDEILI
                     LLKTGLRISEFGGLTLPDLDFENRLVNIDHQLLRDTEIGYYIETPKTKSGERQVPMVE
                     EAYQAFKRVLANRKNDKRVEIDGYSDFLFLNRKNYPKVASDYNGMMKGLVKKYNKYNE
                     DKLPHITPHSLRHTFCTNYANAGMNPKALQYIMGHANIAMTLNYYAHATFDSAMAEMK
                     RLNKEKQQERLVA"
BASE COUNT         6004 a         3254 c         3755 g         5198 t
ORIGIN      
        1 aatttcaact aatattattg tgtaaaggat aataggctgt gattgtctaa actcaattac
       61 cattcataaa gattttcaag aaaaataata aataattttg gggacaaatg atgggacaaa
      121 tcctcttctg tcaagcaaaa aagcctttaa ataaaggctt tttcttatgt agtttaatgc
      181 ccctacaaag taactatatg tatttaaagt taatgtataa aaaaagaaaa caagtaatat
      241 caaggaattt atcacttttt tatataaaaa tagcataaaa atctagttat ccgcataaaa
      301 actggactta tcacacttta tcaaggtcaa aaccactcaa tttactacta atttactact
      361 tatgaatgag ctttgatacg acgatttatc cttgaaaagt gaagatataa agatacttcc
      421 aataaaattt gaatatttaa taggtagaca cttcaaaaaa tgaggtgtct atttttttac
      481 ccgattttga aaggaagtga acttatgaaa acaaaaaatc aagaatcaaa aggtcgttcc
      541 ccactcttta agaccatcaa acattcattc agccaataaa aaagaaagga taggtaaaaa
      601 tatggaactt aaatttgtga ttcccaacat ggaaaaaaca ttcggcaatt tagaatttgc
      661 tggcgaggat aaagtcgttc agcgaagaat caacggacgg ctaactgtct tatcaagaag
      721 ctataatctc tattctgatg ttcaaagagc agatgatatt gtggtggtgc ttcctgctga
      781 agctggcgaa aaacatttcg gctttgagga acgtgtgaag ttagtcaatc cacgtattac
      841 cgcagagggc tacaaaatcg gcactcgtgg ttttacaaat taccttttac atgctgacga
      901 catgataaaa gaataaagaa agagaggaaa aatgatgaga ttagcaaatg gcattgtatt
      961 agataaagac acgacttttg gagaattgaa attctctgct ctacgtcgtg aagtgagaat
     1021 ccaaaatgaa gacgggtcgg tttcagatga aatcaaggaa cgtacctatg acttaaaatc
     1081 caaaggacaa ggacgcatga ttcaagtaag tattcctgcc agcgtgcctt tgaaagagtt
     1141 tgattataac gcacgggtgg aacttatcaa tcccattgcg gacaccgttg ctactgccac
     1201 ctatcaagga gcagatgttg actggtatat caaggcagac gatattgtgc tgacaaagga
     1261 ttctagttca ttcaaagctc aaccacaagc aaagaaagaa ccgacacaag acaaatagtc
     1321 gctaggtaga aaggagactt tttcgcatga aacagcgtgg taaaaggatt cgcccatctg
     1381 gtaaagattt agtctttcat tttacgatag cgtcactcct gcctgttttc ctgctggttg
     1441 tcggactgtt tcatgtgaag acaatccagc agatcaactg gcaggatttt aacctatcac
     1501 aagcagataa gattgacatt ccctatttaa ttatcagttt cagtgtcgca attcttatct
     1561 gcttgctggt agcgtttgta ttcaaacggg ttcgctatga tacggttaaa caactttacc
     1621 accgtcaaaa actggcaaag atgatacttg aaaacaagtg gtatgaatct gaacaggtca
     1681 aaacagaggg ttcctttaaa gatagtgctg gtcgtacaaa ggaaaagata acctacttcc
     1741 ctaaaatgta ttatcgactt aaaaatggct tgatacagat acgggtggaa atcacgctgg
     1801 gaaaatatca agaccaactc ttacacttgg aaaagaaatt agagagtggc ttgtactgtg
     1861 agctgacgga taaagagtta aaggattcct atgtggaata tactttgctc tatgacacca
     1921 tagccagtcg tatttctatt gatgaagtag aagctaaaga tggtaaactt cgcttaatga
     1981 aaaacgtatg gtgggaatat gataagctcc ctcatatgtt gattgctggt ggtacaggtg
     2041 gcggtaaaac ttactttata ctgacactga ttgaagcctt gcttcataca gattcaaaac
     2101 tgtatattct tgacccgaaa aatgctgatc ttgcggactt aggtctcccg gcacacttct
     2161 taatctttga tgaatacgtc gctttcatgg aaatgctggg aacaaaagaa aacaccgcag
     2221 ttatgaataa gctgaaacag attgtcatgt taggtcgtca agctggcttc tttctaatac
     2281 tggcttgtca acgtccagac gcaaaatatt taggcgacgg aatccgtgat cagtttaatt
     2341 tcagagtggc tttaggtcgt atgtctgaaa tgggctatgg catgatgttt ggcagtgacg
     2401 tacaaaagga tttcttctta aagcgaatca aaggtcgtgg ctatgttgat gtaggaacaa
     2461 gtgtcatatc agagttttat actccccttg taccaaaagg atatgatttc ttggaggaaa
     2521 ttaaaaagtt atccaacagc agacagtcca cgcaggcgac gtgcgaagcg gaagtcgcag
     2581 gtgtggactg atcttgctgg ctggtgtggc aatagccacg ccagcactta accccccgta
     2641 tctaacaggg gggtacaaat cgacaggaaa cagtcaaaaa aacattagaa aatcctttgg
     2701 ttacaaggga tttacaaaat ttcagcgtat gtcaaatggg ctttaaaagt tgacatacgc
     2761 ctttttgatt ggagggattt ttactgaatg aacaaacttg gttacagcat ttaaaagaaa
     2821 aacgcttggc ttatggacta tctcaaaacc gtttagctgt tgcgactggt attacaaggc
     2881 agtatctaag cgatattgaa acaggaaaag tcaagccatc agaggattta cagcagtccc
     2941 tttgggaagc tctggaacgc ttcaatcccg acgctcccct tgaaatgctg tttgattatg
     3001 taagaattcg ctttccgaca acagacgtac agcaggtggt cgaaaacatc ttacaactga
     3061 aactgtccta ttttcttcat gaggactatg gtttctattc ttattcagag cattatgctt
     3121 taggcgacat attcgtcctt tgctcccatg aactggacaa aggagttctg gtggaattga
     3181 aaggtcgtgg gtgcagacaa tttgaaagct atcttctggc acaacaaaga agctggtatg
     3241 agttctttat ggacgttttg gtggctggcg gtgtgatgaa acgccttgac cttgccatta
     3301 acgataagac agggatttta aatatccctg tactcactga aaagtgccaa caggaagaat
     3361 gtatctccgt cttccgcagt tttaaaagct atcgcagtgg cgaactggta cgcaaagagg
     3421 aaaaggaatg tatgggaaac accctctata tcggttcatt acaaagtgaa gtttatttct
     3481 gtatctatga aaaggactac gagcagtaca agaaaaatga tattcccatt gaagacgcag
     3541 aagtaaaaaa ccgttttgag attcgattga aaaatgagcg tgcctattat gcagtccgtg
     3601 atttactcgt ctatgacaat ccagagcata ccgcctttaa aattatcaat cggtatatcc
     3661 gttttgtaga taaagacgat tccaaacctc gttctgattg gaaactgaat gaagaatggg
     3721 cttggtttat tgggaacaat cgtgaacgat taaaactaac cacaaaacca gagccttact
     3781 ccttccaaag gacgctgaac tggctatctc atcaagttgc cccgacctta aaggttgcga
     3841 ttaaacttga tgaaatcaac cagacgcagg ttgtaaaaga cattctcgac catgcgaaac
     3901 tgacagaccg acacaagcag attttgaagc aacagtcagt aaaagaacag gacgtgataa
     3961 caacaaaaaa ataactcaaa tacaaattca ttgaatatag agaggagaac atttttatga
     4021 attttggaca aaacctttat aactggtttc tatcaaacgc tcaatcactg gtgcttttag
     4081 caatcgttgt gattggcttg tatcttggct tcaagcgtga gtttagcaaa ctgattggct
     4141 ttttaattat tgcgattatt gcggttggct tagtcttcaa cgctgctgga gtaaaagaca
     4201 ttttactaga gctattcaat cgcattattg gtgcttaaat aaaaccgttc ttttgtggaa
     4261 tataagtggt tttcttatgt tccgcaaagg aatggtacac caaacgaagt gcggtaggga
     4321 tttttgaatc tctacaaaga aaggacgtga atatatggac gatatgcaag tctatattgc
     4381 gaatttaggc aaatacaatg agggcgaatt ggtcggtgcg tggtttacct ttcccattga
     4441 ctttgaggaa gtcaaagaga aaatcggctt gaatgatgaa tatgaggaat acgccattca
     4501 tgactacgag ttacccttta cggttgacga atacacttcc attggcgaac tcaatcgact
     4561 atgggaaatg gtatcggaat tacccgaaga attacaatcg gagctatctg ctctgctcac
     4621 tcatttttca agcattgaag aactaagcga acatcaagag gatattatca ttcattccga
     4681 ttgtgatgat atgtatgacg tggcacgcta ctacattgaa gaaacgggtg ctttaggcga
     4741 agtaccagct agtcttcaaa actatattga ttatcaagcc tatggtcggg atttagacct
     4801 ttcaggaacg tttatctcaa ccaatcatgg gatttttgaa atcgtctatt aaatctgtcg
     4861 gtacattact actggcagat tttctatttt acggggtggc tcaatcagct acccctattt
     4921 tttatgaaag gattgattac atgaagaaaa tacgaagcta taccagtatc tggtctgtgg
     4981 aaaaggtact gtattctatc aatgatttta gacttccgtt tcccataacc tttacgcaaa
     5041 tgacatggtt tgtcgtgtca ctctttgcag tgatgatact tggcaacttg ccccctcttt
     5101 ccatgataga gggagcattt ctcaaatact ttgggattcc tgtggctttc acatggttta
     5161 tgtctacaaa aacttttgat ggtaaaaagc cttatggatt tttgaagtct gtcattgctt
     5221 atgcactgcg accaaagctg acctatgcag gaaaaaaagt aacgcttggc agaaaccagc
     5281 cacaagaagc cattacagca gttaggagtg aattttatgg catatccaat taaatacatt
     5341 gaaaacaatc tcgtctggaa taaagacggg gaatgttatg cttactatga gcttgttcct
     5401 tacaattact catttctaag tccagaacag aaaatacaag tgcatgattc tttcagacag
     5461 cttatcgcac aaaatcgtga tggcaaaatt catgctttac aaatcagtac agaatccagc
     5521 atacgttctg cacaagagcg ttccaaaaat gaagtcactg gcaagctcaa agcggttgcc
     5581 tatgacaaaa tcgaccaaca gacagacgct ttaatatcca tgattggcga aaatcaagtg
     5641 aactaccgtt tctttatcgg ctttaagttg cttctcaacg atcaggagtt ttctatgaaa
     5701 agtcttaccg ttgaagcaaa aaatgctttg tctgattttg tctatgatgt gaaccataag
     5761 ctgatgggcg attttgttag tatgagtaat gatgaaatcc tgcgttttca gaagatggaa
     5821 aagctcttag aaaataaaat ctctcgtcgt ttcaaaatcc gcaggttaga taaggacgac
     5881 ttcggctatc tgattgaaca cctttacgga cagacaggca ctgcctatga agagtatgag
     5941 taccatctat caaagaaaaa gctggataat gaaacgctga ttaaatacta tgacttgatt
     6001 aagcctactc gctgtttggt ggaagaaaaa cagcgatatt tgaaaatcca gcaggaagat
     6061 gaaaccgtct atgtagctta ctttaccatt aacagcattg tcggagaact ggacttcccg
     6121 tcctctgaaa tcttctacta ccagcaacag caatttacat tcccgattga tacgtcaatg
     6181 aatgtggaaa ttgtagcgaa tcgtaaagcc ctatctactg tccgcaataa aaagaaagaa
     6241 ctgaaagact tggataacca cgcttggcaa agtgataatg aaaccagctc caatgtggcg
     6301 gaagctctgg aaagtgtgaa tgagctggaa accaatttag accaaagcaa ggaatctatg
     6361 tacaagctgt cttatgtggt aagggtatca gcaaatgatc ttgacgaact caaacgtcgt
     6421 tgtaatgaag tgaaagattt ttatgacgat ttaagcgtaa aactggtacg accatttggg
     6481 gatatgctcg gcttacatga agaattttta cctgccagca agcgttatat gaatgattat
     6541 attcaatacg tgacctctga tttcctcgct ggtttaggtt ttggtgctac tcaaatgctg
     6601 ggggaaaatg aggggattta tgttggctac agcttagata ctggacgcaa tgtctatctg
     6661 aaacctgctc ttgccagtca aggggttaag ggttcagtaa ccaatgcgtt agcgtcggct
     6721 tttgttggtt cgctgggtgg tggtaaatcc tttgcgaata accttatcgt ctattatgcg
     6781 gtgctttatg gggcacaagc agtgattgta gacccaaaag cagaacgtgg cagatggaaa
     6841 gaaaccttgc cagagatttc ccatgaaatc aatatcgtca ctctgacttc tgatgagaaa
     6901 aacaaaggct tacttgaccc ttatgtgatt atgaaaaatc ccaaagattc tgaatcactg
     6961 gctattgata ttctgacatt ccttacgggg atttcctctc gtgatgggga acgcttccca
     7021 atccttagaa aagccattcg tgcagtaacc aatagtgaag tacgagggtt gatgaaagtg
     7081 attgaggaat tacgggttga gaatacgcca ctaagtacca gtatagccga ccatatcgaa
     7141 agttttacag actatgactt tgcacattta ttattcagta atggttatgt ggagcagtct
     7201 atcagcttag aaaaacaact gaacattata caggttgcgg acttggtact tcccgacaag
     7261 gaaacttcct ttgaggaata taccactatg gagcttttat ccgttgctat gctgattgtc
     7321 attagtacct ttgctttaga ctttatccat acagaccgaa gcattttcaa gattgtagat
     7381 ttagacgaag catggagctt tttacaggta gcacaaggaa aaacactatc tatgaagctg
     7441 gttcgggctg gtcgtgctat gaacgctggg gtatatttcg tgacccaaaa tacagacgac
     7501 ctcttagatg aaaaactgaa aaataacctc ggcttaaaat ttgcatttcg ttccactgac
     7561 cttaacgaga ttaaaaagac cttagccttt tttggtgtag acccagagga cgaaaacaat
     7621 cagaagcgat tgcgtgattt ggaaaacggg caatgcctta tcagtgattt atatggtcgt
     7681 gtcggtgtga tacagttcca ccctgtattt gaagaactgc tccatgcctt tgataccaga
     7741 ccacctgtgc gaaaagaggt gtaaatgtga aaccatcaat agtaaacaga ataaaatcaa
     7801 actggacgct gaaacgtcta ggtaaagtgg caatgacagt ggctttcaca cttgtgattg
     7861 ccatttttct tttagccatg ctgggaacgg tggttcaagc tgcgggcttg gtagatgata
     7921 cggtcaatgt ggcaaatgaa tacagccgat acccacttga aaactatcaa ctggattttt
     7981 atgtggataa tagctggggc tggcttccgt ggaactggtc ggacgggatt ggaaaacagg
     8041 tcatgtatgg actatatgcc attaccaatt ttatttggac aatcagtttg tatgtttcca
     8101 atgcgacagg ttacttagta caggaagcct attccttaga cttcatttcc gctacagcag
     8161 attccattgg taagaatatg cagaccttag ctggtgtgag tgcaaacgga ttttcaacag
     8221 agggtttcta tgttggattc ctcttactct tgattttggt tcttggggtt tatgttgcct
     8281 atacgggact gataaagaga gaaaccacaa aggcaattca tgccattatg aattttgtgc
     8341 tggtgtttat cctatcggct tcctttattg cctacgctcc cgactacatt aaaaaaatca
     8401 atgacttttc atcagacatc agtaatgcca gtttatcact tggcacgaag attgtcatgc
     8461 cccattccga tagtcaaggc aaggacagcg tggacttaat cagagatagc ctgttttcca
     8521 tacaggttca gcaaccgtgg ctactgcttc aatacaacag ttcagacatt gaaagtatcg
     8581 gtattgaccg tgtggaaagc ctgctctcca ccagcccaga ttccaacaat ggcgaagaca
     8641 gagaaaaaat tgttgcggaa gaaattgaag acagaagcaa taccaatcta accattacaa
     8701 agaccattaa ccgtttaggt acagtcttct tcctatttgt cttcaatatt gggatttcca
     8761 tatttgtatt cctattaaca ggaatcatga ttttctcgca ggtacttttt atcatctatg
     8821 ctatgtttct gcctgtgagc tttattttaa gcatgattcc atcatttgat ggtatgtcaa
     8881 aacgagccat aacaaagctc tttaatacca ttttgacacg agctggaatc acattgatta
     8941 ttacgacagc atttagtatt tcaaccatgc tctatacctt atcggctggt tatccgttct
     9001 ttttgattgc ttttctacag attgtgacct ttgcaggaat ctacttcaag ctgggcgatt
     9061 taatgagtat gttttctcta cagagtaacg attctcaaag tgtgggaagt cgtgtgatga
     9121 gaaaacctcg tatgcttatg cacgctcaca tgcaccgtct acagcggaaa cttggacgtt
     9181 ccatgactac tctaggggct gggtctgcca ttgttacagg taaaaaagga cagtcgggtt
     9241 cggggagttc tgcaaggaca caagcagatc actcccgacc agacggaaag gaaaaatcaa
     9301 cacttggaaa acgtatcggt caaaccatcg gtacagtagc tgataccaaa gacagaatgg
     9361 tagacactgc tagtggtttg aaagaacagg ttaaagattt gccgaccaat gcaagatatg
     9421 cagtatatca aggaaaatcc aaagtaaaag agaatgtccg tgatttaacc agtagtattt
     9481 ctcaaaccaa agcggacaga gccagtggac gcaaggaaca gcaggaacaa aggcgaaaaa
     9541 ccattgcgaa gcgtcgctct gaaatggaac aggtcaaaca gaaaaaacag cctgcttctt
     9601 ctgttcatga aagaccgact acaagacaag aacaatatca tgatgaacag acctcaaaac
     9661 agtctaatat tcagacttca tataaggaat ctcaacaagc caaacaagag cgtccagcag
     9721 ttaagtccga tttttcaagt ccaaaagtgg aacgccaagg caataccgtt caagaaaaaa
     9781 ccgttcaaaa gccagcaact tcaaccacta cagcagatag aacttcacaa cgtccaatca
     9841 caaaagaacg tccgtctact gttcaaagag taccactaca aaatacaaga agtagaccac
     9901 caatcaaaac cgccaccatt aagaaagtcg gtaagaaacc atgaagttga aaactttagt
     9961 gattggtggt tctggattat tcttgatggt cttctcactg cttctgtttg ttgccatttt
    10021 attttcagat gaacaggaca gcggaatttc caatattcat tatggaggtg tgaatgtttc
    10081 cgcagaagtg ctggctcata agcctatggt agaaaaatat gccaaagaat atggcgttga
    10141 agaatatgtc aacatacttc ttgcgattat acaggtggaa tcgggcggta ctgcggaaga
    10201 tgttatgcag tcctcggaat ccctcggtct tccacctaat tcattgagta cagaagaatc
    10261 cattaagcaa ggtgtgaagt atttcagtga attattagcc agtagcgaaa ggctcagtgt
    10321 agatttagaa tcggttatcc agtcctacaa ttatggtggt ggtttcttag ggtatgtggc
    10381 taatcgtgga aataaatata cctttgaact ggctcaaagt ttctcaaaag agtattcagg
    10441 tggcgaaaaa gtgtcttacc ccaatcccat agccatacct atcaatgggg gctggcgata
    10501 caactatggc aatatgtttt atgtgcaact ggtaacgcag tatcttgtca caacagagtt
    10561 tgatgatgat acggtacaag ccatcatgga cgaagcactg aaatatgagg gctggcgata
    10621 cgtttacggt ggagcttccc cgactacttc ttttgattgt agcggactga cacaatggac
    10681 gtatggaaaa gctggaatta acttaccacg aaccgcacaa cagcaatatg atgtgaccca
    10741 gcatatccca ctatcggaag cacaagctgg cgatttggtt ttctttcatt ctacctataa
    10801 cgctggctct tatattactc atgttgggat ataccttggc aataaccgta tgtttcatgc
    10861 aggcgaccca atcggttatg ccgacttaac aagcccctac tggcaacagc atttagtggg
    10921 agcaggacga atcaaacaat gagaaaggaa gatttaatga tgaaatttag aaaaaatcag
    10981 aataaagaaa aacagatacc aaaggaaaag aaacctcgtg tctataaggt caatcctcat
    11041 aaaaaggttg tgattgcctt gtgggtactt ttagggctta gtttcagctt tgcgatattc
    11101 aagcacttta cagctataga tactcatact attcacgaaa caactatcat agaaaaggaa
    11161 tacgttgata ctcatcatgt agaaaatttt gtagagaact ttgcgaaagt ctactattca
    11221 tgggagcaat ccgataagtc cattgataat cgaatggaaa gtctaaaagg ctatctgaca
    11281 gatgaacttc aagctctcaa tgttgataca gtacgcaaag atattcctgt atcgtcttct
    11341 gtaagaggat ttcagatatg gacggtagag ccaactggcg acaatgagtt taatgtaacc
    11401 tacagtgtag accagctcat tacagaggga gaaaatacaa agaccgtcca ctctgcttat
    11461 atagtgagtg tctatgtaga tggttctgga aatatggtac tggttaagaa tccgaccatt
    11521 accaacatac ctaagaaatc aagttataaa ccaaaagcca ttgaaagtga ggggacggtt
    11581 gattccatta caaccaatga aatcaatgag tttttaacga cgttcttcaa gctctatcct
    11641 acagcgacag ccagtgaact ttcctactat gtgaatgacg ggatattaaa accaatcgga
    11701 aaagagtaca tctttcaaga actggtaaat cctattcaca atcgtaagga taatcaagtc
    11761 acggtatcgc tgacagtgga gtatatcgac cagcagacca aagcaacgca ggtatctcaa
    11821 tttgatttgg tacttgaaaa gaacgggagt aattggaaga ttgtaaaata acaaatattg
    11881 gtacatgatt acagatactt tgtaatcatg tactcttttt gataaaaaat tggagattcc
    11941 tttacaaata tgctcttacg tgctattatt taagtgacta tttaaaagga gttaataaat
    12001 atgcggcaag gtattcttaa ataaactgtc aatttgatag cgggaacaaa taattagatg
    12061 tcctttttta ggagggctta gttttttgta cccagtttaa gaataccttt atcatgtgat
    12121 tctaaagtat ccagagaata tctgtatgct ttgtatacct atggttatgc ataaaaatcc
    12181 cagtgataaa agtatttatc actgggattt ttatgccctt ttgggttttt gaatggagga
    12241 aaatcacatg aaaattatta atattggagt tttagctcat gttgatgcag gaaaaactac
    12301 cttaacagaa agcttattat ataacagtgg agcgattaca gaattaggaa gcgtggacaa
    12361 aggtacaacg aggacggata atacgctttt agaacgtcag agaggaatta caattcagac
    12421 aggaataacc tcttttcagt gggaaaatac gaaggtgaac atcatagaca cgccaggaca
    12481 tatggatttc ttagcagaag tatatcgttc attatcagtt ttagatgggg caattctact
    12541 gatttctgca aaagatggcg tacaagcaca aactcgtata ttatttcatg cacttaggaa
    12601 aatggggatt cccacaatct tttttatcaa taagattgac caaaatggaa ttgatttatc
    12661 aacggtttat caggatatta aagagaaact ttctgccgaa attgtaatca aacagaaggt
    12721 agaactgtat cctaatatgt gtgtgacgaa ctttaccgaa tctgaacaat gggatacggt
    12781 aatagaggga aacgatgacc ttttagagaa atatatgtcc ggtaaatcat tagaagcatt
    12841 ggaactcgaa caagaggaaa gcataagatt tcataattgt tccctgttcc ctgtttatca
    12901 cggaagtgca aaaaacaata tagggattga taaccttata gaagtgatta cgaataaatt
    12961 ttattcatca acacatcgag gtccgtctga actttgcgga aatgttttca aaattgaata
    13021 tacaaaaaaa agacaacgtc ttgcatatat acgcctttat agtggagtac tacatttacg
    13081 agattcggtt agagtatcag aaaaagaaaa aataaaagtt acagaaatgt atacttcaat
    13141 aaatggtgaa ttatgtaaga ttgatagagc ttattctgga gaaattgtta ttttgcaaaa
    13201 tgagtttttg aagttaaata gtgttcttgg agatacaaaa ctattgccac agagaaaaaa
    13261 gattgaaaat ccgcaccctc tactacaaac aactgttgaa ccgagtaaac ctgaacagag
    13321 agaaatgttg cttgatgccc ttttggaaat ctcagatagt gatccgcttc tacgatatta
    13381 cgtggattct acgacacatg aaattatact ttctttctta gggaaagtac aaatggaagt
    13441 gattagtgca ctgttgcaag aaaagtatca tgtggagata gaactaaaag agcctacagt
    13501 catttatatg gagagaccgt taaaaaatgc agaatatacc attcacatcg aagtgccgcc
    13561 aaatcctttc tgggcttcca ttggtttatc tgtatcaccg cttccgttgg gaagtggaat
    13621 gcagtatgag agctcggttt ctcttggata cttaaatcaa tcatttcaaa atgcagttat
    13681 ggaagggata cgctatggtt gcgaacaagg attatatggt tggaatgtga cggattgtaa
    13741 aatctgtttt aagtatggct tatactatag ccctgttagt accccagcag attttcggat
    13801 gcttgctcct attgtattgg aacaagtctt aaaaaaagct ggaacagaat tgttagagcc
    13861 atatcttagt tttaaaattt atgcgccaca ggaatatctt tcacgagcat acaacgatgc
    13921 tcctaaatat tgtgcgaaca tcgtagacac tcaattgaaa aataatgagg tcattcttag
    13981 tggagaaatc cctgctcggt gtattcaaga atatcgtagt gatttaactt tctttacaaa
    14041 tggacgtagt gtttgtttaa cagagttaaa agggtaccat gttactaccg gtgaacctgt
    14101 ttgccagccc cgtcgtccaa atagtcggat agataaagta cgatatatgt tcaataaaat
    14161 aacttagtgt attttatgtt gttatataaa tatggtttct tgttaaataa gatgaaatat
    14221 tttttaataa agatttgaat taaagtgtaa aggaggagat agttattata aactacaagt
    14281 ggatattgtg tcctgtatgt ggaaataaaa cacgattaaa gataagggaa gatactgaat
    14341 taaaaaaatt ccccctctat tgtccgaaat gcagacaaga aaatttaatt gaaataaagc
    14401 agttcaaagt aactgtgatt acagagccag acgcaaagac gcagagccga taaaatgaga
    14461 ttaatacaat ctcattttat cggctctttc cgttatgtat ggattctttt aattagtctt
    14521 cgatgtttct tgcttcgttg ataccgctgg ctaaagattc cattaaggat agttctttgt
    14581 ctgtaaagct atccatgtat ttctctatct gtaatcgtcg ggtgcttttt accaagttat
    14641 tagcaggtaa gaaaaattca tcaacggaaa catgaagtaa cgatacaagg tcataaagaa
    14701 cttgtatgct ggggtgttgc cctttatttt caatattagt taagtaccgt gggtcaattt
    14761 caatcaatgc tcccacttgt tcacgagtta aacctcgttt caatcgagct tctttaatgg
    14821 ctaaaccaaa ggctctaaaa tcatatttat cttctttttt acgcatagta gaccacctct
    14881 atacatttta ttgttcctac tgaattaaaa acaggtatag aaaaacgtgt tatatggttt
    14941 ataggtttat atttaataaa aagcactact aaacgccaat aaaaaaaacc gttatatggt
    15001 agtgctattt acgctgttaa aatattgtat attacttcca aatggcggtt tgttggaggt
    15061 caacgtcgcc atgaagtaca tcatatacaa taaatttcct tacattgggt tcttgtcaaa
    15121 aaaagtcgtc tatctgcaat agataagtac gtccaccaat gtggttttat aaatcatata
    15181 gatagaataa cagaagcatg taaacagaga aataaatctg tttatatgct tttttggcta
    15241 ttcagaactt ttttacaaag tttatttatc agtaatgcaa caaatccccc tttcacattg
    15301 ggactaagag tgaaaggaga taaacgagca aggctcactt cctttcctag acagaaaggg
    15361 ggtgagaaac atgaaaccat cttcttttca gaccacaata gaaaatcagt ttgactatat
    15421 ctgtaaacgt gctatggaag acgagcgaaa gaattatatg ctttatcttt caaggattgc
    15481 aaagcgtgag gtgtcctttt cggatgttgg cgattatctt gttagccagt ttgcgacaac
    15541 agataactat tcaactgact ttcagatttt tacactcaat gggttatcag taggcgttga
    15601 aaatgatttg ttgagtgaag cattacgtga gttgccagac aagaaacgtg aaattctact
    15661 gctgttttac tttatggaca tgagcgattc agaaattgca gacctgttga aattgaaccg
    15721 ttctactgtc tatcggcata gaaccagtgg actagcctta attaaaaagt ttatggagga
    15781 atttgaagaa tgaaaacaca atatcctatg attccctttc ctctcattgt aaaggcaaca
    15841 gatggcgata ccgaagcgat taaccagatt ctacatcatt acagagggta cataacgaag
    15901 cgttccctac gacttatgaa agatgaatat ggcaatcaaa gtatggtcgt tgatgaagtc
    15961 ttacgtggaa gaatggaaac cagactgatt acaaagattt tgtcatttga aattaagtaa
    16021 tatcctctct cctttcgtgg aagcgtgcta aaccattcca cgcttcccga acagggaggt
    16081 ttgttattcc accaaagcat attgagcttt caatgtgttt tgataggcta acgagccatt
    16141 gttctttgaa aactgaataa aagtaatcga atacgtttcg ataagaaaag agccaacgga
    16201 actaaccgcc atgacctatc ttataaagat agcgagcgat tcatgttagt gatccgagaa
    16261 gcaatcttta gcaggattgc ctgcaacgac attcttatcg tgataatgat actcccatac
    16321 agtcaatagt ccgagcgtga taaaaccgtc gcaggcaatg agtatggcta catgagaacc
    16381 atgcaggggt ggaactcccg tgagctttgc taaagctgtt cgattgctgg taaaacaact
    16441 tttatgaaat ccaaataagt gatttggaaa ggaggatttt atgaagcaga ctgacattcc
    16501 tatttgggaa cgttataccc taaccattga agaagcgtca aaatattttc gtattggcga
    16561 aaacaagcta cgacgcttgg cagaggaaaa taaaaatgca aattggctga ttatgaatgg
    16621 caatcgtatt cagattaaac gaaaacaatt tgaaaaaatt atagatacat tggacgcaat
    16681 ctagcgtcgc caaagggtct tgtatatgat aaaatagtat taagtcgtat caaggctctt
    16741 tccataaagg aaaggagcaa atgccatgtc agaaaaaaga cgtgacaata aaggtcgaat
    16801 cttaaagact ggagagagcc aacgaaaaga cggaagatac ttatacaaat atatagattc
    16861 atttggagaa ccgcaatttg tttactcgtg gaaacttgtg gctacagacc gagtaccagc
    16921 aggaaagcgt gattgtatct cacttagaga gaaaatcgca gagttacaga aagacattca
    16981 tgatggtatt gatgttgtag gaaagaaaat gacactctgc cagctttacg caaaacagaa
    17041 cgctcaaaga ccaaaggtta gaaaaaacac tgaaactgga cgcaaatatc ttatggatat
    17101 tttgaagaaa gacaagttag gtgtaagaag tattgacagt attaagccat cagacgctaa
    17161 agaatgggct attagaatga gtgaaaatgg ttatgcttat caaaccatca ataactacaa
    17221 acgttcttta aaggcttcat tctatattgc tatacaagat gattgtgttc ggaagaatcc
    17281 atttgacttt caactgaaag cagttcttga tgatgatact gtccctaaga ccgtactaac
    17341 agaagaacag gaagaaaaac tgttagcctt tgcaaaagct gataaaacct acagcaaaaa
    17401 ttatgatgaa attctgatac tcttaaaaac aggtcttcgt atttcagagt ttggtggttt
    17461 gacacttcca gatttagatt ttgagaatcg tcttgtcaat atagaccatc agctattgag
    17521 agatactgaa attgggtact acattgaaac accaaagacc aaaagtggcg aacgtcaagt
    17581 tcctatggtt gaagaagcct atcaagcatt taagcgagtg ttagcgaatc gaaagaatga
    17641 taagcgtgtt gagattgatg gatatagtga tttcctcttt cttaatagaa agaactatcc
    17701 aaaagtggca agtgattaca acggcatgat gaaaggtctt gttaagaaat acaataagta
    17761 taacgaggat aaattgccac acatcactcc acatagtttg cgacatacat tctgtaccaa
    17821 ctatgcaaat gcaggaatga atccaaaggc attacagtac attatgggac atgctaatat
    17881 agccatgacg ctgaactatt acgcacatgc aacattcgat tctgcaatgg cagaaatgaa
    17941 acgcttgaat aaagagaagc aacaggagcg tcttgttgct tagtagtaca aatgaattta
    18001 ctacttattt accacttctg acagctaaga catgaggaaa tatgcaaaga aacgtgaagt
    18061 atcttcctac agtaaaaata ctcgaaagca catagaataa ggctttacga gcatttaaga
    18121 aaatataaaa agataattag aaatttatac tttgtttgtt tataaaaatt aataaagaaa
    18181 gataaatttt ctagaacaaa tctagaacaa a
//
LOCUS       DATRMA010000036        18136 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.036, whole genome shotgun sequence.
ACCESSION   DATRMA010000036 DATRMA010000000
VERSION     DATRMA010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18136
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.036"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            59..403
                     /gene="fbsA"
                     /locus_tag="VBC28_001179"
                     /pseudo
     CDS             59..403
                     /gene="fbsA"
                     /locus_tag="VBC28_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000482192.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding adhesin FbsA"
     gene            612..1688
                     /locus_tag="VBC28_001180"
     CDS             612..1688
                     /locus_tag="VBC28_001180"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEO6815170.1"
                     /translation="MGYTVAIVGATGAVGTQMIRQLEQSNLPIEQVKLLSSSRSAGKI
                     LHFKDEAIRVEETTKESFYDVDIALFSAGGSISAKFAPYAVKSGAVVVDNTSYFRQNP
                     DVPLVVPEVNAHAMIGHNGIIACPNCSTIQMMIALEPIRQKWGIERVIVSTYQAVSGS
                     GARAVEETKEQLRQVLNDNLSPDQLIATVLPCSSDQKHYPIAFNALPQIDIFTDNDYT
                     YEEMKMTLETKKIMEDATIKVSATCVRIPVLSGHSESIYIETKELASISEIKKAIANF
                     PGAVLQDLPSQQIYPQAINAVGHRETFVGRIRKDLDQENGVHMWVVSDNLLKGAAWNS
                     VQIAETLHKNGLVKPAKELKFELI"
     gene            1858..2196
                     /locus_tag="VBC28_001181"
     CDS             1858..2196
                     /locus_tag="VBC28_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001165748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6815171.1"
                     /translation="MQIIKRDGQIVEFDPEKIYQAILKAAQTVYVLDESLRQDLAQVT
                     KKVILDLEDAQVERPTINMIQSLVENRLLDAGYIHTAEHYISYRLQRDLERNGYDDQI
                     TVHLHFEKIK"
     gene            2327..3868
                     /locus_tag="VBC28_001182"
     CDS             2327..3868
                     /locus_tag="VBC28_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000025412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEO6815172.1"
                     /translation="MSILEVKNLSHGFGDRAIFENVSFRLLKGEHIGLIGANGEGKST
                     FMSIVTGHLQPDEGKIEWSKYVTAGYLDQHTVLEEGQTVRDVLRTAFDELFKTEARIN
                     DIYISMADDGADVDSLMEEVGELQDRLESRDFYTLDAKIDEVARALGVMDFGMDSEVT
                     ELSGGQRTKVLLAKLLLEKPDILLLDEPTNYLDAEHIDWLKRYLQNYENAFVLISHDI
                     PFLNDVINIVYHVENQDLVRYSGDYTNFESVYAMKKAQLEAAYERQQKEIADLQDFVN
                     RNKARVATRNMAMSRQKKLDKMDIIELQAEKPKPSFEFKESRTPGRFIFQAKDLQIGY
                     DRALTKPLNLTFERNQKIAIVGANGIGKTTLLKSLLGIIPPISGNVERGDFIDLGYFE
                     QEVPGGNRQTPLEAVWDAFPALNQAEVRAALARCGLTSKHIESQIQVLSGGEQSKVRF
                     CLLMNRENNVLVLDEPTNHLDVDAKDELKRALKAYKGSILMVCHEPDFYEGWMDDVWD
                     FNQLS"
     gene            3908..5140
                     /locus_tag="VBC28_001183"
     CDS             3908..5140
                     /locus_tag="VBC28_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001178658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEO6815173.1"
                     /translation="MQNDMSVVLRAGKLLIESGAEVYRVEDTMKHFAKALQIENFEAY
                     VVSSSIIASGINRYGKQEAKVCNTDGVTANLGRLEAVNNLSRQIAKQDLVSPEEIVKQ
                     LDLIEHQKDYSLLVTLISYFCGAGSFSLALGSSLLDSFSAAVTGLILGYFLNLMESRI
                     HTGFLLTILGSSVVALSANLLYFSGLGEHRSIIILGALMVMVPGAAFVNSVREFSQNN
                     FSTGLALIMSALLICISISAGVAITIEIIPFAEQMTGSFSGVPNTILEIIIRTLMAGL
                     GTIAFSILYHVPKRYFLDLGILGAISWMLYLILWQQFHMDAIAVFFPGLFITYFSRLL
                     AAKRKCPATVFLATSMFPLIPGLSFYRAVYFLLTGADAVAMEYFRSCFVTAFTIAIAI
                     SIVQQIPLSFFIRRKMIK"
     gene            5353..6054
                     /gene="pyrF"
                     /locus_tag="VBC28_001184"
     CDS             5353..6054
                     /gene="pyrF"
                     /locus_tag="VBC28_001184"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEO6815174.1"
                     /translation="MLEKCPIIALDFSDLASVTTFLEHFPKEELLFVKIGMELYYSEG
                     PSIIRYIKSLGHRIFLDLKLHDIPNTVRSSMSVLAKLGIDMTNVHAAGGVEMMKAARE
                     GLGEGPILLAVTQLTSTSQEQMQVDQHINLSVVDSVCHYAQKAQEAGLDGVVASAQEV
                     KQIKKQTNEHFICLTPGIRPPQTNQLDDQKRTMTPEQARIVGADYIVVGRPITKAENP
                     YQAYLEIKEEWNRIK"
     gene            6067..6696
                     /locus_tag="VBC28_001185"
     CDS             6067..6696
                     /locus_tag="VBC28_001185"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEO6815175.1"
                     /translation="MDLARQIAMELLDIQAVYLRPQQPFTWASGVKSPIYTDNRVTLS
                     YPETRTLIENGFVKQIQKHFPNVDIIAGTATAGIPHGAIIADKMNLPFAYIRSKAKDH
                     GVGNQIEGRVYSGQKMVIIEDLISTGGSVLEAVTAAQSQGIEVLGVVAIFTYQLAKAE
                     QAFREADIPLVTLTDYNQLIKVAKVNGYITADQLVLLKKFKEDQMNWQS"
     gene            6708..8000
                     /locus_tag="VBC28_001186"
     CDS             6708..8000
                     /locus_tag="VBC28_001186"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEO6815176.1"
                     /translation="MYIIKNGLIIDPQSGFNQVSDMLIDQGKIKQISKEIDIKGIPII
                     DASNKIVAPGLVDIHVHFREPGQTHKENIHTGALSAAVGGFTTVLMMANTNPTISSPE
                     IVKQVKESAAKEAIKIETVATITKSLNGKDLVNFEELLEAGVAGFSDDGIPLTDTKVL
                     QEAMNLARKHDVVLSLHEEDPSLNGVLGINEHIAQKIYHVCGASGLAEYSMIARDAMI
                     AYQTQAKVHIQHLSSSESVEVVDFAQKLGANLTAEVTPQHFSKTENLLLTKGANAKLN
                     PPLRLEKDRQALIDGLKSGVISIIASDHAPHHIMEKAADNISQAPSGMTGLETSLALG
                     ITYLVSTKELSMIDFLAKMTCNPAQLYGFDAGYLREGGPADIVIFDQAEERIIKAEFA
                     SKSSNSPFIGDKLKGVIHYTICNGEIVYQKDSHAATLV"
     gene            8165..9088
                     /locus_tag="VBC28_001187"
     CDS             8165..9088
                     /locus_tag="VBC28_001187"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001016473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEO6815177.1"
                     /translation="MNHQTQTLSLEHFVSLEELSNQEVMSLIKRSIEVKENPSNIGFD
                     KDYYVSNLFFENSTRTHKSFEMAELKLGLKTIEFNADTSSVNKGETLYDTILTMSALG
                     LDVCVIRHPDIDYYKELIASPNIHSAIVNGGDGSGQHPSQSLLDLVTIYEEFGYFKGL
                     KIAIVGDLTHSRVAKSNMQVLKRLGAEIFFSGPKEWYSSQFDEYGQYLPIDQLVDQID
                     VLMLLRVQHERHDGKGVFSKESYHQQFGLTKERYKHLRDTAIIMHPAPVNRDVEIASD
                     LVEADKARIVKQMSNGVYARIAILEAVLNSR"
     gene            9102..10178
                     /gene="carA"
                     /locus_tag="VBC28_001188"
     CDS             9102..10178
                     /gene="carA"
                     /locus_tag="VBC28_001188"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000826106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEO6815178.1"
                     /translation="MKRLLLLEDGSVFEGEAFGADVETSGEIVFSTGMTGYQESITDQ
                     SYNGQIITFTYPLIGNYGINRDDYESIRPTCKGVVIYEWAEYPSNWRQQMTLDEFLKL
                     KGIPGISGIDTRALTKIIRKHGTMKACLINEGNSIHEALENLQKSVLLNDQIEQVSTK
                     LAYASPGVGKNIVLVDFGLKHSILRELSQRQCHITVVPHTTTAQEILNLNPDGVLLSN
                     GPGNPEQLPNALQMIQEIQGKIPIFGICMGHQLFAKANGAKTYKMTFGHRGFNHAVRH
                     LQTGQVDFTSQNHGYAVSREDFPEALFITHEEINDKTVEGVRHKYYPAFSVQFHPDAA
                     PGPHDTSYLFDEFINMIDDFQQKS"
     gene            10209..13391
                     /gene="carB"
                     /locus_tag="VBC28_001189"
     CDS             10209..13391
                     /gene="carB"
                     /locus_tag="VBC28_001189"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEO6815179.1"
                     /translation="MPKRTDIRKIMVIGSGPIVIGQAAEFDYSGTQACLSLKEEGYQV
                     VLVNSNPATIMTDKDIADKVYIEPITLEFVTRILRKERPDALLPTLGGQTGLNMAMAL
                     SKNGILEELNVELLGTKLLAIDKAEDRDLFKQLMEELNQPIPESEIVNSVEEAIQFAE
                     QIGYPLIVRPAFTLGGTGGGMCDNQEQLVDITTKGLKLSPVTQCLIERSIAGFKEIEY
                     EVMRDAADNALVVCNMENFDPVGIHTGDSIVFAPAQTLSDVENQLLRDASLDIIRALK
                     IEGGCNVQLALDPNSFKYYVIEVNPRVSRSSALASKATGYPIAKLAAKIAVGLTLDEV
                     INPITKTTYAMFEPALDYVVAKMPRFPFDKFESGDRKLGTQMKATGEVMAIGRNIEES
                     LLKACRSLEIGVDHIKIADLDNVSDDVLLEKIRKAEDDRLFYLAEALRRHYSIEKLAS
                     LTSIDSFFLDKLRVIVELEDLLSKNRLDINILKKVKNKGFSDKAIASLWQINEDQVRN
                     MRKEAGILPVYKMVDTCASEFDSATPYFYSTYAVENESLISDKASILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIRESGFEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIDL
                     EKPEGVILQFGGQTAINLAKDLNKAGVKILGTQLEDLDRAENRKQFEATLQALNIPQP
                     PGFTATTEEEAVNAAQKIGYPVLVRPSYVLGGRAMKIVENEEDLRHYMTTAVKASPDH
                     PVLIDAYLIGKECEVDAISDGQNILIPGIMEHIERSGVHSGDSMAVYPPQTLSETIIE
                     TIVDYTKRLAIGLNCIGMMNIQFVIKDQKVYVIEVNPRASRTLPFLSKVTHIPMAQVA
                     TKVILGDKLCNFTYGYDLYPASDMVHIKAPVFSFTKLAKVDSLLGPEMKSTGEVMGSD
                     INLQKALYKAFEAAYLHMPDYGNIVFTVDDTDKEEALELAKVYQSIGYRIYATQGTAI
                     YFDANGLETVLVGKLGENDRNHIPDLIKNGKIQAVINTVGQNNIDNHDALIIRRSAIE
                     QGVPLFTSLDTAHAMFKVLESRAFTLKVLD"
     gene            13518..13841
                     /locus_tag="VBC28_001190"
     CDS             13518..13841
                     /locus_tag="VBC28_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000921242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6815180.1"
                     /translation="MLKTRTHINELETKIALDLVHRYLGIQQLVFSKWRHSLETGSWS
                     SIITINGVNIINYIVDDISDSNSISGIICHPKSFHLRPREKKEPIQSIDELDITWSLN
                     REENE"
     gene            13838..14620
                     /locus_tag="VBC28_001191"
     CDS             13838..14620
                     /locus_tag="VBC28_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001923787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxcM-like domain-containing protein"
                     /protein_id="HEO6815181.1"
                     /translation="MIPDSSFKVLSDMAYNIEKSKAESNGHLQIIEGSQIIDFHQKRS
                     FMVLEVNDDINNGMQAMACQSLDESNHIVIAYSGTNAFNKKDILTDIDSVVLGKNNKL
                     LTCPSSITLREDGISLPCKNPSQITTSKLFAQKIKMNYPDATLWLTGHSLGGYLAMIV
                     ASEMRLSARVFNSPSPINNMSEAATSYVISHPEKYHSYRINKDYIGNFGINSSDDKLG
                     ISKRVNAKKQHGFYKKIFFHELTRFRLNQQGQIVDIDENKQD"
     gene            14737..15027
                     /locus_tag="VBC28_001192"
     CDS             14737..15027
                     /locus_tag="VBC28_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WXG100 family type VII secretion target"
                     /protein_id="HEO6815182.1"
                     /translation="MAQIKLTPEELRSSAQKYTAGSQQVTEVLNLLTQEQAVIDENWD
                     GSTFDSFEAQFNELSPKITEFAQLLEDINQQLLKVADIIEQTDADIASQISG"
     gene            15109..18129
                     /gene="esaA"
                     /locus_tag="VBC28_001193"
     CDS             15109..18129
                     /gene="esaA"
                     /locus_tag="VBC28_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EsaA"
                     /protein_id="HEO6815183.1"
                     /translation="MKRNNFRILWYIIAVALFLVAIAGLNLKLQGDHAKENKTTQSAT
                     NTKLNIALVNEDQNVSNGKESYNLGASYIKSIERDNSQNWSVVSRGTAQNGLDKGDYQ
                     LMVIIPNNFSQKLLDVNKANAEQTTISYKVNAKGNLALEKKATEKEKDIVSELNSHLV
                     NMYMASILSNLYTAQENVQAMVNVQSGNISNYQKNLLDSATNFQNIFPALVNQSSSSI
                     TANESLKKSLEASDNMFNDLVTTQTNTGKDLSSLIEQRHQDSISYEAFSTSLLEMNNE
                     LLEKQLSDIITQAQKDQETLSSQLNSIMGDDNNHNHKENSSAYLNVARQKIQELSEAL
                     KSQDNIAKDQSEQLDKIVREGLASYFAKNNKDNITLLELLKSHSTNEKTLKDFKAKVA
                     DFTNSLISSIPSLNLSELHLTQEEEKAIQFTSSDSEIIKKVSNERTLYLNTNLLNRLF
                     EARKNRDEAKNKVNQLKLSSSSTRTGEQIVSVESNNPDYRVDTWTVNGKQTRTLDPSQ
                     NNNIIINSSYQRESSNSSITKPSYTITIGNQKQIVQNDDGKIQRSYFEAEATYQRTLQ
                     EVNDAYNTTHGLVAKYYIISDGEEPQNLFDQFLNQSVNDTMVDLVKNGITKYLMDENT
                     ADAQQKVKDVMEEVENSQDGLADQMAKVTETNVRLTDAIKKQLETLQSINMKVQNITQ
                     DQSKVNDSQKTTDQQLSDLKNQLDGLMTSAAGVKDMSKSNSQEADQVNQIFTSFNKDV
                     QDAKNSGNKLSTDATDLMANFQKELANNGDFVASFSKVFNTAYKNGVPNDILLNFLSR
                     PVAESASAVRATENTYRPFTWILLLEVVSLFTAYIFATQNLIKKLTDRYNVNRWLQTD
                     FLNVIVISGLSLVIGLALGVISSRSLHVMPEYVPSWFLVMTLFSFLLIHSQYFFIKNF
                     KAVGMGLALFMIISFVYLSNAVGTVATVSGLPKLLKAINPLSILENQLSSYFDNVTTG
                     FIFLILVLLVDVAFIIMNIFITLNFEAKVKEWGC"
BASE COUNT         6197 a         2883 c         3460 g         5596 t
ORIGIN      
        1 atttaaaaaa agtaaggaga aaattaattg ttcaataaaa taggttttag aacttggaaa
       61 tcaaagtcag ggcaatgttt tagagcgtcg tcaacgcgat gttgagaaca agagccaagt
      121 aggtcaactt atagggaaaa atccacttct ttcaaagtca attatatcta gagaaaataa
      181 tcactctagt caaggtgact ctaacaaaca gtcattctct aaaaaagtat ctcaggttac
      241 taatgtagct aatagaccga tgttaactaa taattctaga acaatttcag tgataaataa
      301 attacctaaa acaggtgatg atcaaaatgt catttttaaa cttgtaggtt ttggtttaat
      361 tttgttaaca agtcgctgcg gtttgagacg caatgaaaat taagtataat caatcattta
      421 gtaactatat ataatgatat atgcaatcaa taaaaaggaa tcggatacga gattcctttt
      481 tataattagg ttggttaggg tgactttttt catttggcta ttcttgaaag tttataaaaa
      541 tgtagtataa tagtcacatt aaaatgtttt gaaaatattg atgaacaaca tcaacaaata
      601 gaggtcatta tatgggatat accgttgcta tcgtaggtgc tacaggtgcc gtaggaacac
      661 aaatgattcg tcaattagaa caatcgaatt taccaataga acaagtgaaa cttttatcat
      721 caagtcgctc agcaggtaaa attttacatt ttaaagatga ggctatacgt gttgaagaga
      781 caacaaaaga atcattttac gatgttgata ttgccttgtt ttcagctggt ggatctattt
      841 cagcaaagtt cgctccttat gcagtaaagt ctggagcagt tgtagtagat aacacgtcat
      901 attttcgtca gaatcctgat gttccactag ttgttcctga agtaaatgct catgccatga
      961 ttggtcataa tggtatcata gcttgcccca attgttctac tattcaaatg atgattgctt
     1021 tagagcccat tcgtcaaaaa tgggggatag agcgtgttat agtttccacc tatcaagctg
     1081 tttcgggttc aggtgcacgt gctgttgaag aaactaagga acagttgaga caagttttaa
     1141 atgataacct ttcacctgat caattaattg caacagtctt gccatgttct agcgatcaaa
     1201 aacattatcc tatcgcattc aatgcattac ctcaaattga tatttttact gataatgatt
     1261 atacgtacga agaaatgaaa atgactttgg agacaaagaa aattatggaa gatgctacta
     1321 ttaaggtttc agctacctgc gtccgtattc ccgttttatc ggggcattct gagtccattt
     1381 atattgagac gaaagaatta gcctcaatct ctgaaattaa aaaggctatt gcaaattttc
     1441 ctggtgccgt cctacaagac cttccatctc agcaaattta ccctcaagcg attaatgctg
     1501 taggccatag ggagaccttt gttggtcgta ttcgaaaaga ccttgatcaa gaaaatggtg
     1561 ttcatatgtg ggtagtttca gataatctac tgaagggggc agcttggaat tctgttcaaa
     1621 ttgcagaaac attgcataag aatgggctgg ttaaaccagc aaaagagtta aaatttgaat
     1681 taatatagaa gaatatgaca ttgcttaata cttatgctga ctgctagtga tattgatttt
     1741 tcgtcaatat caggtaaaac acttgacaaa ttatgaaaac atttgcataa tgaaattaac
     1801 aaatgaaaat gattgcaaat cgttttaaca ttgtttccac attaaggagg ggctcttatg
     1861 caaatcatca aacgtgatgg acagattgtt gaatttgatc ctgaaaaaat ctaccaagct
     1921 atcttgaaag ctgctcaaac agtttatgtg cttgatgagt cacttcgtca agaccttgct
     1981 caagtgacta aaaaagtaat cttagattta gaggatgctc aggttgaacg accaacgatc
     2041 aatatgattc agtctttggt tgaaaatcgt ttattggatg caggatacat tcatactgct
     2101 gaacactata tttcataccg tttgcaacgt gatttagagc gaaatggcta tgatgatcaa
     2161 atcactgttc atttacactt tgaaaaaatt aagtaaggtt tgacttttaa gtttaaactc
     2221 tctatacttg tatatggaac ttactatgta atgtactaat ttaaaaactt gtagagtgtc
     2281 tctatgagtt ttttaataac ctctattatc aatcaaagga ttttaaatga gtattttaga
     2341 agtaaaaaac ttaagtcacg gttttggtga tcgtgctatt tttgagaatg tttcttttcg
     2401 ccttttaaaa ggtgaacata taggacttat tggtgctaat ggtgaaggaa aatcaacatt
     2461 catgtcaatt gtcacaggtc atcttcaacc tgatgaagga aaaattgaat ggtcaaaata
     2521 tgtaactgct ggttaccttg atcaacatac tgtattagaa gaaggtcaaa cagttcgtga
     2581 tgttttgcga acagcatttg atgaactttt taaaacagag gcacgtatca atgatatata
     2641 tatatccatg gcagatgatg gcgcagatgt agatagcctt atggaagaag ttggagaact
     2701 acaagatcgt ttagaatcac gtgattttta tacacttgat gctaaaattg atgaagttgc
     2761 acgggcatta ggagttatgg attttggtat ggattctgaa gttactgaat tatctggtgg
     2821 tcaaaggaca aaggttcttt tagcaaaact gttattagaa aagccagaca tcctcctgct
     2881 tgatgagcct acaaactacc ttgatgctga acacattgat tggttaaaac gctatttaca
     2941 aaattatgaa aatgcttttg ttttaatctc acacgatatt ccatttttga acgatgttat
     3001 caatattgtc tatcatgttg aaaatcaaga tttagtaaga tattccggtg attatactaa
     3061 ttttgaatca gtctatgcaa tgaaaaaggc tcaattagaa gcagcctatg aacgtcaaca
     3121 aaaggaaata gctgatttac aagattttgt taatcgtaat aaagcacgcg tcgcaacacg
     3181 taatatggca atgtctcgtc aaaagaaact agacaaaatg gatattattg agttacaagc
     3241 agagaaacca aagccaagtt tcgaatttaa agaatctcgc acaccaggac gttttatttt
     3301 ccaagctaaa gatcttcaaa ttggatatga tcgtgcttta acaaaacctc taaacctaac
     3361 atttgaacgt aatcaaaaaa tagctattgt tggagcaaat gggataggaa aaacaacatt
     3421 acttaagagt ttactaggta ttatcccccc tatttctggt aatgttgaac gaggtgattt
     3481 tattgattta ggttattttg aacaagaagt acccggagga aatcgccaaa ctccactcga
     3541 agcagtatgg gatgcttttc ctgctttgaa ccaagcagag gttagggcag ctcttgctcg
     3601 ttgtggttta acttcaaagc atattgaaag tcagattcag gtgttatctg gtggggagca
     3661 atcaaaggtc agattttgct tattaatgaa tcgcgaaaat aatgtccttg tacttgacga
     3721 accaaccaat catttagatg tagatgctaa agatgagtta aaacgtgctt taaaagctta
     3781 taagggatca atcttgatgg tatgtcatga accggacttt tatgaaggat ggatggatga
     3841 tgtttgggac tttaaccaac ttagttaagt ggctaaggta ataaagtaga aggcagggcc
     3901 aaataagatg caaaatgata tgagcgttgt tcttagagca ggtaaattac tgattgaaag
     3961 cggagcagaa gtctatcgtg tcgaagacac aatgaagcat tttgctaagg ctcttcagat
     4021 agaaaatttt gaagcttatg tggttagtag cagtattatc gcttccggga ttaaccgcta
     4081 tggtaaacaa gaagctaaag tatgcaatac agatggtgta acagcaaatt taggtagact
     4141 tgaagctgtt aataaccttt ctaggcaaat tgctaaacaa gacttagtat ctccagaaga
     4201 aattgtaaag cagttagact taattgaaca tcaaaaagat tatagtttac ttgttacttt
     4261 aatttcttat ttttgtggag cgggtagttt ttctttagcc ttaggtagtt ccttattaga
     4321 ttcattttcc gctgctgtaa caggcttaat tttaggatat tttctgaatt tgatggaaag
     4381 tcgtatacat acaggatttc ttttgaccat tctaggtagt tctgtagtgg ctttaagtgc
     4441 taatttatta tatttttcag gattaggtga gcatcgtagc attattatat taggggcact
     4501 aatggttatg gtgccagggg ctgcctttgt taattcggtg agagaatttt cacagaataa
     4561 tttttcaaca ggtttagctt tgattatgtc tgctttactc atttgtattt ctatatcagc
     4621 aggtgtggct attacaattg aaattattcc gtttgcagaa caaatgacag gttctttttc
     4681 tggtgttcct aataccatcc ttgagattat catcagaaca ctaatggccg gtttagggac
     4741 gatagccttc tctattctct accatgttcc aaaacgttat ttccttgact taggtatact
     4801 aggagcaata tcttggatgc tctacctgat cttatggcaa caatttcaca tggatgctat
     4861 agccgtattt tttccagggc ttttcatcac ttatttttca cggctattag ctgctaaacg
     4921 taagtgtcct gccactgtct ttttagcgac aagtatgttt cctcttattc caggacttag
     4981 cttctataga gctgtttact ttttattgac gggagcagat gccgtagcaa tggaatattt
     5041 tagatcctgt tttgtaacag cttttacaat tgctatagca atcagtattg tacagcagat
     5101 tcctctatcg ttctttatta gacgtaaaat gataaaataa tttagtattt atttgacaaa
     5161 atgattgtaa acttgtagaa tacctacaaa tgtatcttta agtgagtcca gagaggcgaa
     5221 caaggtcacc atcataagtc aaatagcgag tgctttttcg ctaacttttt atgaaacctt
     5281 gccctcaggc aaggtttttt gtgtcatact ggtacatttc caacaattat tatttctata
     5341 gagaggaatt atatgttaga aaaatgccct atcattgcct tagatttttc tgatttggct
     5401 tctgtaacaa cttttttaga acattttcca aaagaagaat tgctttttgt caaaatagga
     5461 atggagcttt attattccga aggtccctca attattagat atattaaatc cttaggacat
     5521 cgcatttttt tggatttaaa attacatgat atccctaata cagttcgatc atctatgtct
     5581 gtcttagcca aattaggcat tgatatgact aatgtgcatg ctgcaggagg agtcgaaatg
     5641 atgaaagcag ctagagaagg tttgggggag ggtccaatac tattagcagt gacacaatta
     5701 acctcgactt cacaagaaca aatgcaggtg gatcaacata ttaacttaag tgtagttgat
     5761 tcggtttgcc attatgctca aaaagctcaa gaagcaggct tagatggagt agtcgcatcg
     5821 gctcaagagg taaagcaaat caagaaacag actaacgaac atttcatttg cttaacgcct
     5881 ggtatcagac ctccacaaac caatcagtta gatgatcaaa aaagaacgat gactcctgag
     5941 caagcacgta tagttggggc tgattatatc gttgtgggaa gacctataac gaaagcagaa
     6001 aatccatatc aagcatatct agagataaaa gaagaatgga atcgtattaa ataaaggagt
     6061 actgagatgg atttagcaag acagattgcc atggaactat tagatattca agctgtttac
     6121 ttaagacccc aacaaccctt tacttgggct tcgggggtca aatcgccaat ttacactgat
     6181 aatcgtgtaa ccttatcgta tcctgagaca aggacactga ttgaaaatgg ttttgttaaa
     6241 caaatacaga aacatttccc aaacgttgat atcatagcag ggacagcgac tgcaggcata
     6301 ccacatggcg ctattatagc tgataagatg aacttgccct ttgcctatat acgatcgaag
     6361 gcaaaggatc atggtgtggg gaatcaaatc gagggacgtg tttactcagg ccaaaaaatg
     6421 gttatcattg aagatttgat ttcaacagga ggatctgttt tagaggctgt tacggctgcg
     6481 cagagtcaag gtattgaggt tttaggggta gtagcgatat ttacatatca attagctaag
     6541 gctgaacaag catttcgtga agctgatatt cctttggtga ctttgacgga ttacaatcaa
     6601 ttaattaaag ttgctaaagt taatggatat attacagctg accaactggt attactgaaa
     6661 aaatttaaag aagatcaaat gaattggcaa agttagagag gacagatatg tatatcatta
     6721 aaaacggatt gattatcgac cctcaaagtg gttttaatca agtatcagac atgctgattg
     6781 atcaaggaaa aattaaacaa atatcgaaag aaattgatat taaaggcatt ccaatcattg
     6841 atgcaagcaa taaaattgta gctccaggat tagtcgatat tcatgtacat tttcgtgagc
     6901 ctggacagac acataaagaa aatatccata caggggccct atcagctgca gtaggtggtt
     6961 ttacgacagt tcttatgatg gccaatacta acccaacgat ttctagccca gaaattgtaa
     7021 aacaagttaa ggagagtgca gcaaaagaag caattaagat tgaaacagtt gctacaatta
     7081 ctaaatctct gaatggtaaa gatttagtta atttcgaaga actattagag gctggagttg
     7141 ctggattctc agacgatggt attcctttga cagatactaa ggttttgcaa gaagctatga
     7201 acttagctag aaaacatgat gttgtcctat cgttacatga agaagatcca tcgttaaatg
     7261 gtgtccttgg tatcaacgaa catattgctc agaaaattta tcatgtctgt ggagctagtg
     7321 gtcttgctga atattcaatg attgcgcgag atgctatgat tgcttaccaa acacaggcga
     7381 aagtccatat ccagcatttg tcaagttcag aatcagtaga agtagttgat tttgcgcaga
     7441 agcttggtgc taacttaaca gcagaagtaa ccccccaaca tttttcaaag acagagaact
     7501 tacttttaac taagggtgct aatgcaaaat taaacccacc actgcgactt gaaaaggata
     7561 gacaagccct cattgatgga cttaaaagtg gtgttatttc aattattgca agtgaccacg
     7621 ctccccatca tattatggag aaagcagcag ataatattag tcaagcgccc tctggtatga
     7681 ctggcttaga gacatcatta gccttaggga ttacttattt agtatctact aaagaactct
     7741 caatgattga ctttttggca aaaatgacat gtaatcctgc acagttatat ggatttgatg
     7801 caggttatct cagagaaggt ggacctgcag atatcgttat ttttgatcaa gctgaagaga
     7861 gaataataaa ggcagaattt gcctccaaat ccagcaactc tccttttatc ggtgataaat
     7921 tgaagggagt tattcactac acaatttgca atggtgaaat agtctatcaa aaagatagcc
     7981 acgcagcgac attggtatag gtaaaacatt tcttattaat atcctttaag gtattgtcca
     8041 gagaggcaaa caaggaattg cgttaacaaa tagtaagtta gttccctatc tagcttacta
     8101 ttttgcagag cctccttgtt ttaggaggct tttttctagc taaaatttat agaaagaaat
     8161 tatcatgaat catcaaacac aaacactttc attagaacat tttgtcagtt tagaagaatt
     8221 atcaaatcaa gaagttatgt ctttgataaa gagaagtatc gaagttaaag aaaatcctag
     8281 caacattggt tttgataaag attactatgt gtcaaatctc ttttttgaga attcaacaag
     8341 aacacataag tcatttgaaa tggcagaact caaattagga ttaaaaacaa ttgaatttaa
     8401 tgctgataca agttctgtta ataaaggaga aaccctttat gacactatct taacaatgag
     8461 tgctctagga ttggacgttt gtgtcattag gcatcctgat attgactatt ataaagaatt
     8521 gattgctagt ccgaacattc attcagctat tgtcaatggg ggtgatggct caggccaaca
     8581 tccaagccaa tcacttttgg atttggtaac tatttacgaa gaatttggat acttcaaagg
     8641 gctaaaaatt gcgattgtag gagatttaac tcactcacgg gtagctaaat ctaacatgca
     8701 agtgctaaaa cgattaggag ctgaaatttt cttttctgga cccaaagaat ggtatagtag
     8761 tcaattcgat gaatatgggc aatatttacc aatagatcag ttagttgatc aaattgatgt
     8821 ccttatgctt ttacgtgttc aacacgaacg tcatgatggt aaaggggtgt tttcaaaaga
     8881 aagctatcat caacaatttg gtcttacaaa agaacgctat aaacatttaa gggatacagc
     8941 aattattatg cacccagcac ccgtcaatag ggatgtagag attgctagcg atttggtaga
     9001 agcagataag gctcgaatag tgaaacagat gagtaatggc gtctatgcaa ggatagctat
     9061 cttagaagca gtgctcaata gcagataata aaggagataa aatgaagcgt ctattattac
     9121 tggaagatgg tagtgtgttt gagggtgagg ctttcggagc ggatgtagaa acaagtggtg
     9181 aaatcgtttt tagtacagga atgacagggt atcaagaatc tataacagat caatcttaca
     9241 acggtcaaat tattactttt acttatcctc ttattggaaa ttatgggata aatcgagacg
     9301 attacgaatc tattagacca acatgtaagg gagtagttat ttatgaatgg gcagagtatc
     9361 caagtaactg gcgtcaacag atgacacttg atgaattttt aaaattaaaa ggaatacctg
     9421 gaatttcagg tatagatact cgagcattaa caaaaattat tcgaaaacat ggaacaatga
     9481 aagcttgttt aataaacgag ggaaattcta ttcatgaagc actagaaaac cttcaaaaaa
     9541 gtgttctttt aaatgatcaa attgaacaag tatcaaccaa attagcttat gcttcgcctg
     9601 gagttggcaa aaatattgta ttagttgact ttggtttaaa acattctatt ctaagagaac
     9661 tgtcacaacg tcaatgtcat attacagtcg ttcctcatac tacgacagcg caagaaatct
     9721 taaatctcaa tccagatgga gtactcttat ctaacggccc tgggaaccca gaacaattac
     9781 cgaatgcttt acaaatgatc caagaaattc aaggtaaaat tccaattttt ggtatttgta
     9841 tgggacatca actatttgct aaagctaatg gtgcaaagac ttataaaatg acttttggtc
     9901 atcgaggttt taatcatgct gttcgtcatt tgcaaacagg acaggttgat tttacaagtc
     9961 aaaatcatgg ttatgctgtc tcgagagaag atttccctga ggctctcttc attacacatg
    10021 aagagattaa tgataaaact gtcgaaggtg ttcgacataa atactatcct gccttttcag
    10081 tgcagtttca cccagatgca gcaccagggc cgcatgatac atcttatctt tttgatgaat
    10141 ttattaatat gattgatgat ttccagcaaa aaagctagct ttaaaatata gaattagagg
    10201 agtttgatat gccaaagcgc acagacatac gtaaaattat ggttatcggt tctggcccga
    10261 ttgtgattgg acaagcagca gagtttgact attctggtac acaagcctgt ctctctttaa
    10321 aagaggaggg ataccaagtt gttttggtca attcgaatcc agctactatc atgacagata
    10381 aagatattgc tgataaagtt tatattgaac ctattacctt ggagtttgtc acaagaattc
    10441 tcaggaagga aagacctgat gctctccttc caactcttgg aggtcaaact ggattgaata
    10501 tggctatggc tctatcaaaa aatggtatcc tagaagaatt gaatgttgaa ctattaggga
    10561 ccaaattatt agccattgat aaagctgaag atcgtgattt atttaaacag ttgatggaag
    10621 aactcaatca acctattcca gaatctgaga ttgttaattc ggtagaagaa gctattcaat
    10681 ttgcagagca aatcgggtat ccattaattg ttcgtcctgc ctttacttta ggaggtacag
    10741 gtggaggcat gtgcgataat caagaacaat tggttgacat cacgacgaag gggttaaagt
    10801 tatcgcctgt gacacagtgc cttattgaac gttcaattgc tggctttaaa gagattgaat
    10861 acgaagttat gcgtgatgca gcagataacg ctcttgttgt ttgtaatatg gagaattttg
    10921 atcctgttgg tatccataca ggggactcta tagtttttgc acccgcacaa acgttatctg
    10981 atgtggaaaa tcagttatta cgagatgcga gtttagatat tataagagct ttgaaaattg
    11041 aaggaggatg caatgttcaa ctagcccttg atccgaatag ttttaaatac tatgtcattg
    11101 aagttaatcc aagagtatca agatcttctg ctttagcttc aaaagctact ggatatccaa
    11161 ttgctaaatt agcagctaag atagctgttg gtttgacact agatgaggta attaatccta
    11221 ttacaaaaac gacttacgct atgtttgaac ctgctttaga ttacgtagtc gcaaaaatgc
    11281 cgagatttcc ttttgataaa tttgaaagtg gcgatagaaa attgggaaca cagatgaaag
    11341 ctacaggcga agtcatggca ataggacgga atatagaaga atctttgttg aaagcttgtc
    11401 gttcactaga aataggtgtc gatcacatta aaatagcaga tttagataat gtatcagatg
    11461 atgttttatt agaaaaaata agaaaggcag aagatgatcg cttattttat ctagcagaag
    11521 ctttacgtag acattatagt attgaaaaat tagcaagttt gactagcatt gattctttct
    11581 tccttgataa gttaagagtg attgtagagt tagaagatct attatctaaa aacagactgg
    11641 atatcaatat tctcaaaaag gttaaaaata aaggtttttc agataaagct atagcgagtt
    11701 tatggcaaat aaacgaagat caagttcgta acatgcgaaa agaagcagga attcttccag
    11761 tttataagat ggttgataca tgtgcgtctg agtttgattc agcaacgccc tacttttatt
    11821 cgacttatgc cgtagaaaac gagtcattga tatcagataa agcctctatt ttggttttag
    11881 gatcgggccc aatccgaatt ggacagggag ttgaatttga ttatgcaacg gttcattctg
    11941 ttaaagccat cagagagtct ggttttgaag cgattatcat gaactctaat ccagagacgg
    12001 tctcaacgga tttctctatt tcagataagc tttactttga acctttgact tttgaagatg
    12061 ttatgaatgt tattgactta gaaaaacctg aaggggtcat cttacaattc ggtggtcaga
    12121 ctgctattaa tctagcaaaa gatttaaaca aggcaggggt taaaatacta ggcacacaat
    12181 tagaagattt ggatcgtgct gaaaatagaa aacaatttga agccacttta caagctctaa
    12241 atatccctca accaccaggt tttaccgcca ctacggagga agaggcagtt aatgcagcgc
    12301 aaaagattgg ttatcccgtg ttggttagac catcatatgt tcttggtgga cgagctatga
    12361 agatagttga aaacgaagaa gatttacgtc attatatgac aaccgcggta aaggcaagcc
    12421 cagatcatcc agtgcttata gatgcatacc ttataggtaa agaatgtgag gttgatgcta
    12481 tatccgatgg tcagaatata ctgattcctg gaataatgga acatatagag cgatctggtg
    12541 ttcattcagg agattccatg gctgtatatc ctcctcaaac attgtctgag accattatcg
    12601 aaactattgt agattataca aaacgcttag caattggatt aaactgtatc ggtatgatga
    12661 atattcagtt tgtaatcaag gatcagaaag tatatgttat tgaagttaat cctcgtgcta
    12721 gtagaacact tccattctta tcaaaagtaa ctcacattcc tatggcccaa gtagcaacaa
    12781 aagttatctt aggagataaa ctctgcaatt ttacatatgg ttatgatctc tatccagctt
    12841 cagatatggt ccacattaaa gcccctgtct ttagttttac caaacttgct aaagtagata
    12901 gtttattagg tcctgaaatg aaatcaacag gcgaagtgat gggatcagat attaatcttc
    12961 agaaagcctt gtataaagcc tttgaagcag catatctcca tatgccagac tatggtaaca
    13021 ttgtttttac agtagatgat acagataaag aagaagcttt agaacttgca aaagtctatc
    13081 aaagtattgg ttatcgtatt tatgctaccc aagggactgc aatttatttt gatgctaatg
    13141 gtttagagac agttttagta ggtaagttag gagagaacga tcgaaatcat attcctgatt
    13201 taattaaaaa tggtaagatt caagcagtta tcaacacagt tggacaaaat aacattgata
    13261 accatgatgc tctcatcatc agacgttctg caattgaaca aggagttcct ctatttacat
    13321 ctttagatac tgcacatgca atgttcaaag ttcttgaaag cagagcattt acattaaaag
    13381 tactagatta atcaaacgat caaggtaaaa ttaacttcca gcaaaacaga atcggactag
    13441 attaaatata gcttgttctg tttttataaa aaaagcctaa ttgtgataaa atagggaaca
    13501 aacttaagga gttaattatg ttaaaaacac gaacacatat taatgaatta gaaacaaaaa
    13561 tcgcccttga tttagtacac agatatctag gtattcaaca acttgttttt tcaaagtgga
    13621 ggcattcttt agaaactggt tcatggtctt ctattataac aataaatggc gttaatatta
    13681 tcaattacat tgttgatgat attagtgaca gtaatagtat ttcaggtatt atttgtcatc
    13741 ctaaaagttt tcatctcaga cctagagaaa aaaaggaacc tattcagtct attgatgagt
    13801 tagatatcac ctggtctttg aatagagagg aaaatgaatg attccagata gtagttttaa
    13861 agtgctatca gatatggcat ataatattga aaaatccaaa gcagaaagta atggacacct
    13921 tcaaattata gaaggtagtc aaattattga tttccatcaa aaacgctctt ttatggtatt
    13981 agaggttaat gatgatatca ataatggtat gcaggctatg gcatgtcagt ctttggatga
    14041 aagtaatcat attgttatag catattcagg aacgaacgct tttaataaga aagatatctt
    14101 aacagatatc gattcagttg tattggggaa gaacaataaa ttgctaactt gtccttcaag
    14161 tattaccctg agagaggatg gcatttctct gccttgtaaa aatccaagtc aaattacaac
    14221 atctaaacta tttgcacaaa aaattaagat gaactatcct gatgcaaccc tctggttaac
    14281 aggccattct ctaggtggtt atttggccat gattgtggct tcagaaatga gattatcagc
    14341 gcgtgttttt aattccccta gtcccattaa caatatgtct gaagcagcca catcctatgt
    14401 aatttcacat ccagagaagt atcatagtta ccgtattaat aaagattaca ttggtaattt
    14461 tggtattaat agttcagatg ataaattagg tatttctaaa agagtcaatg ctaagaagca
    14521 acatggtttt tataaaaaga ttttctttca cgaattgaca cgatttcgat taaatcaaca
    14581 aggacagatt gttgatatag atgaaaataa acaggattaa acaataacat taaaaataat
    14641 tttaaataat gcagtaaatt tgaatatatt ttaaaaaata tgttgtaata attatgcagt
    14701 tgttgcaaaa gtgtttatag aaaaaggaga aaatttatgg cacaaattaa attaacacca
    14761 gaagaacttc gctcatcagc acaaaagtac acagctggtt ctcaacaagt aacagaagtt
    14821 cttaatttgc ttactcaaga gcaagcagtt attgacgaaa attgggatgg atctactttt
    14881 gattcttttg aagcacaatt caatgaatta tctccaaaaa ttacagaatt tgctcaatta
    14941 ctcgaagata ttaaccagca attattaaaa gttgcagata tcattgagca gacggatgca
    15001 gatattgctt ctcaaattag tggatagtat cgtatcatag tctataagtg aataggaagg
    15061 caaagccttc ctattttgtt gaaaataaaa tatattttag gaagaattat gaaaagaaat
    15121 aactttagaa tactttggta tattatagct gttgctctct ttttagtagc tattgctggc
    15181 cttaatctta agcttcaagg cgatcatgca aaagagaata aaacaacaca gtcagctact
    15241 aacactaaac taaatatagc attagtcaac gaagatcaaa atgtatctaa tggtaaagaa
    15301 agttataatt taggagctag ttatattaag tctatcgaac gtgataatag tcaaaattgg
    15361 tcagttgtta gtcgaggtac tgctcaaaat ggattagaca aaggtgatta tcaattaatg
    15421 gttatcatcc ctaataactt ttctcaaaaa cttcttgatg tcaataaagc aaatgcagag
    15481 caaacaacta tctcttataa ggttaatgcc aaggggaatt tagcattaga gaaaaaggca
    15541 actgaaaaag agaaagatat tgtttcagag ttaaatagcc acttagtaaa tatgtacatg
    15601 gcaagtattt taagtaactt atatacagct caagaaaatg tacaggccat ggtaaatgtt
    15661 cagtcaggta atatctcaaa ttaccaaaaa aatcttttag attctgcaac taatttccaa
    15721 aatatctttc cagccctcgt taaccagtct agtagttcca ttactgctaa cgaatcactg
    15781 aaaaaatctt tagaagcttc tgataacatg tttaatgatt tggtgacaac ccagacaaat
    15841 actggaaaag atttatcaag cttaatagaa cagcgccatc aagatagcat ttcgtatgaa
    15901 gcgttttcga cctcattact agaaatgaat aacgagttat tagagaagca attatctgat
    15961 attatcacac aagcacaaaa agaccaagaa acattatcat cacaactcaa tagtattatg
    16021 ggtgatgata acaatcataa tcataaagaa aactcatcag cttatctaaa tgttgcaagg
    16081 caaaaaatcc aagaactatc tgaagcactc aagtcacaag ataacattgc taaagatcaa
    16141 agtgaacaac tagataaaat tgttagagag ggactagcaa gttactttgc taagaataat
    16201 aaagataata ttactttatt agaattattg aagagtcatt ctactaatga gaagactttg
    16261 aaagatttta aagctaaggt agcagatttc acaaattctc tcatttcaag cattccttca
    16321 ctaaaccttt cggagttgca cttaacccaa gaagaagaaa aagccattca gtttacatct
    16381 tctgattcgg aaatcattaa aaaagtaagc aacgaaagaa ctctgtattt aaatactaat
    16441 ttattgaatc gtctttttga agctaggaaa aatagagatg aagctaaaaa taaagttaat
    16501 caattaaaat tgtcatctag ttctactcga actggtgagc agatagtttc tgttgaatca
    16561 aataatccag attacagggt agatacctgg acggttaatg gtaagcaaac aagaaccttg
    16621 gaccccagcc aaaacaataa tattattatc aatagcagtt atcaaaggga atcatctaac
    16681 tctagtatta cgaaaccaag ctatacaatc actatcggga atcagaaaca aattgtacaa
    16741 aatgatgacg gtaaaatcca acggtcttac tttgaggcag aggcaacgta tcaacgaact
    16801 ctacaagaag taaatgatgc atataacacc actcacggat tagtagctaa atactacatt
    16861 atttctgatg gcgaagaacc gcaaaattta tttgatcaat ttttaaatca aagtgttaac
    16921 gatacgatgg ttgatctggt caaaaatggt ataactaagt atttgatgga tgaaaacact
    16981 gctgatgcgc aacagaaagt taaagatgtc atggaggaag ttgaaaatag tcaagatgga
    17041 ttggcggatc aaatggcaaa agtaactgag acaaatgtac gactaacaga tgcaatcaaa
    17101 aaacagcttg aaacccttca atcaattaac atgaaggtcc aaaacatcac acaggatcaa
    17161 tctaaagtaa acgactcaca gaaaacaact gatcaacaat tatctgattt gaagaaccag
    17221 ctagatgggc taatgacctc tgcagcgggc gtaaaagata tgtccaaatc taatagtcaa
    17281 gaggcggacc aagttaatca aatattcaca tcattcaata aagatgttca agatgctaaa
    17341 aattcgggca acaaactttc gacggatgca accgatttaa tggctaactt ccaaaaagaa
    17401 ttggccaata atggtgattt cgtagcttct ttctctaaag tttttaatac tgcttataaa
    17461 aatggagtgc caaatgatat tctccttaat ttcttatcac gacctgtcgc tgaatcagcg
    17521 tctgcagtta gggcaactga aaatacttat cgcccattca cttggatact attattagaa
    17581 gttgttagcc tatttacagc atatatcttt gcgactcaaa accttattaa gaaattgaca
    17641 gataggtata atgtcaaccg ctggcttcaa acagactttt taaatgtcat tgtcatttca
    17701 ggcctctctc ttgttattgg tttagcactg ggagttatct caagcagaag tcttcatgtc
    17761 atgcctgaat atgtaccatc ttggttccta gttatgactc tgtttagttt tttattaatt
    17821 catagtcagt atttctttat taaaaatttt aaagcagttg gtatgggttt agctttgttt
    17881 atgattatta gttttgtgta tctatcaaat gccgtaggca cagtagcgac tgttagtgga
    17941 cttccaaaat tactaaaagc tattaatcca ctatctatcc ttgaaaatca attatcatct
    18001 tattttgata atgtgacaac tggatttatt ttccttatct tagtacttct tgtggatgtt
    18061 gcttttatta tcatgaatat ttttatcacc ttaaattttg aagctaaagt aaaagagtgg
    18121 ggttgttgat gaagtt
//
LOCUS       DATRMA010000037        18067 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.037, whole genome shotgun sequence.
ACCESSION   DATRMA010000037 DATRMA010000000
VERSION     DATRMA010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18067
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.037"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            <1..1158
                     /locus_tag="VBC28_001194"
     CDS             <1..1158
                     /locus_tag="VBC28_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEO6815184.1"
                     /translation="IKPIIVNKKVSGYAFLLMNGKDFLLPTKAINSDLIIADQLNNSF
                     TFTNRDFISSSLDKVDSQFLTRYFSFHDHRAFVVRKVALQDNILLYMYRPLIPVTLVV
                     LFSLVSSVIIFVILRQKSRVLADRIAVKNSSAINQMVLDMDAISRQEKSSIELDSQDE
                     FQYLSVQINQMVSRLKDLHEKTLDLETQKLLFEKRMLEAQFNPHFLYNTLETILITSH
                     YDSQLTERIVIQLTKLLRYSLSGSTEAAVLKDDLAIIESYLLINQVRFEELTYTISVS
                     PELEHMRVPKLFLLPLIENAIKYGLKERHDVAINIDIWQDSDGIWFTVSNNGSGISLA
                     RQQAIRTMLRSTHSHHGLINSYRRLQYQFSTVLLEFTKTDDAFRVSYIVKE"
     gene            1162..1935
                     /locus_tag="VBC28_001195"
     CDS             1162..1935
                     /locus_tag="VBC28_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEO6815185.1"
                     /translation="MYRLLIVEDEHLIRKWLRYAIDYQSLNILVVGEAKDGKEGAQLI
                     QEEQPDIVLSDINMPIMTAFDMFEATKGQSYAKIILSGYADFPNAQSAIHYGVLEFLT
                     KPLEKQALIDCLKTIMARIEEHKEKHLQEHTELYLPLPQANDQVPEVIKDMLAWIHSH
                     FHGKIVISQLAHDLGYSESYLYTVTKKHLHITLSDYINQYRINQAIQLMFREPDLMVY
                     QIAEAVGIYDYRYFDRVFKKYLGQTVKAFKEEHIFKQMD"
     gene            1947..2984
                     /locus_tag="VBC28_001196"
     CDS             1947..2984
                     /locus_tag="VBC28_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEO6815186.1"
                     /translation="MKEKQSKRLIYILLVVSIIFISVFTYSISQPSKLLPPKELVILS
                     PNSQAILTGTIPAFEEKYGIKVKLIQGGTGQLIDRLSKEGKQLKADIFFGGNYTQFES
                     HKALFESYVSKNVHTVIPDYIHPSDTATPYTINGSVLIVNNELAKGLTIKSYEDLLQP
                     SLKGKIAFADPNTSSSAFSQLTNILLAKGGYTNPKAWNYVKKLQHNINAIKSSSSSEV
                     YQSVAEGKMIVGLTYEDPSVNLQKSGANVSIVYPTEGTVFVPSSVAIIKNAPSMKEAK
                     LFINFMLSLDVQNAFGQSTSNRPIRKDAQTSNGMKALKDIATLKEDYRYVTKHKGQIL
                     KTYNRIRRNAD"
     gene            complement(3206..3703)
                     /locus_tag="VBC28_001197"
     CDS             complement(3206..3703)
                     /locus_tag="VBC28_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HEO6815187.1"
                     /translation="MVATLIIIRGNSASGKSTIAKQLQAELGENTLLLSQDYLRREML
                     GTKDGENTTTIPLLINLLNYGYHNCSYIILEGILRSDWYTPVWKHILKHNPNNTYAYY
                     YDLSFQETVKRHSTRLKSLEFGEDSLARWWLEKDFLKEIPEKILTKAMSLEDVKKLIM
                     TDVLP"
     gene            complement(3703..4740)
                     /locus_tag="VBC28_001198"
     CDS             complement(3703..4740)
                     /locus_tag="VBC28_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HEO6815188.1"
                     /translation="METTLNYIKTLTSIPSPTGFTQTIMTYIIKELEAFGYSPIRTNK
                     GGVMVSLKGKNDTKHRMITAHLDTLGAMVRAIKPDGRLKIDLIGGYTYNAIEGENCTI
                     HLSKNGKEISGTALIHQTSVHVYKDAGTAERNQTNMEIRLDEKVTTADETRALGIQVG
                     DFISFDPRTIITDSGFIKSRYLDDKVSAGILMELLSVYKKEDIQLPYTTHFYFSAFEE
                     LGHGANSSIPNETVEYLAVDMGAMGDDQETDEYTVSICVKDASGPYHYELRQHLVSLA
                     ENNNIPYKLDIYPYYGSDASAAMRAGAEVKHALLGAGIESSHSYERTHIDSIQATELL
                     VDAYLKSNMVD"
     gene            complement(4751..5206)
                     /gene="nrdI"
                     /locus_tag="VBC28_001199"
     CDS             complement(4751..5206)
                     /gene="nrdI"
                     /locus_tag="VBC28_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HEO6815189.1"
                     /translation="MPKLTLVYISLSGNTQSFVKRLSEQLSTWHNCQVSTINIKEQNH
                     QTFPVDQPFVALLPTYLEGGNGIDNGDQEILTIPLGDFIAAHNNVKNCIGIVGSGNRN
                     FNNQYCLTAKQYSERFGFPMLGDFELRGTSSDVERIANIIVETLSNFKA"
     gene            complement(5363..7765)
                     /locus_tag="VBC28_001200"
     CDS             complement(5363..7765)
                     /locus_tag="VBC28_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase/3'-nucleotidase"
                     /protein_id="HEO6815190.1"
                     /translation="MSKHYFSKSVFALTVLTATATSGLAVQAEDIVTTPSSTSTKVES
                     TTPTSTIAEEKSNVTSTPTAITDASTATSTNTTTNNQNPQPVATEATTSDLKPIEGEK
                     VDLRIMSTTDLHTNLVNYDYYQDKESQKIGLAKTAVLIEEAKKENPNTVLVDNGDVIQ
                     GTPLGTYKAIVKPVAENEEHPMYQAMNALGYDASTLGNHEFNYGLDYLKKIIATANLP
                     ILNANVLDFKTHQPVFKTYDIITKTFKDSTGRAVALNIGITGIVPPQILNWDKANLEG
                     KVIVKDSVKAIEEIVPTMRAKGADVILVLSHSGIGDDRYEEGEENVGYQIASIKGVDA
                     VVTGHSHAEFPSGNGTGFYEKYTGVDGINGKINGTPVTMAGKYGDHLGIIDLGLSYTN
                     GKWQVSESSAKIRKIDMNSTTADERIIALAKEAHDGTINYVRQQVGTTTAPITSYFAL
                     VKDDPSVQIVNNAQRWYVANELKGTPEANLPLLSAAAPFKAGTRGDATAYTDIPAGPV
                     AIKNVADLYLYDNVTALLKVTGADLREWLEMSAGQFNQIDPNNKAPQNIINTEYRTYN
                     FDVIDGLTYKFDITQPNKYNKDGKVVNSQASRVRDLMYNGKPVADKQEFMIVTNNYRA
                     SGTFPGAKNATMNRLLNLENRQTIINYIISEKTINPTADNNWGFTESIKDLDLRFQTA
                     DKAKNLVTNSKDIQYIASSTKDEGFGDYRFVYTEQEKVDLTEPSHQEKLSQIEKQKIH
                     HMVATAYTKAKASPNITGTVYTQQKEKTLPKTGQEKQSYLAFFGVAILGLVGSLLKKK
                     QI"
     gene            8025..10241
                     /locus_tag="VBC28_001201"
     CDS             8025..10241
                     /locus_tag="VBC28_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019316080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEO6815191.1"
                     /translation="MVKEINLTGEEVVAITSQYMSETDVAFVKFALNYATAAHYYQAR
                     KSGEPYIIHPIQVAGILADLHLDAVTVACGFLHDVVEDTEITLDEIETDFGKDVRDII
                     DGVTKLGKVEYKSHEEQLAENHRKMLMAMSKDIRVILVKLADRLHNMRTLKHLRKDKQ
                     ERISRETMEIYAPLAHRLGISRIKWELEDLSFRYLNETEFYKISHMMSEKRREREELV
                     DIIVDKIRSYTEEQGLYGDIYGRPKHIYSIYRKMRDKKKRFDQIYDLIAIRCIMETAS
                     DVYAMVGYIHELWRPMPGRFKDYIAAPKANGYQSIHTTVYGPKGPIEIQIRTKEMHQV
                     AEFGVAAHWAYKKGITSKVNQAEQSVGMGWIQELVELQDESKDAKDFVDSVKEDIFTE
                     RIYVFTPNGAVQELPRESGPIDFAYAIHTQVGEKATGAKVNGRMVPLTAKLKTGDVVE
                     IITNPNSFGPSRDWIKIVKTNKARNKIRQFFKNQDKETSINKGRELLVDYFQEQGYVP
                     NKYLDKKHIEEILPRVSVKSEEALYAAVGFGDLSPISIFNKLTEKERREEERAKAKAE
                     ADELINGGEIKTDKRDVLKVKSENGVIIQGASGLLMRIAKCCNPVPGDLIEGYITKGR
                     GVAIHRSDCQNLKSQENYEQRLIDVEWDDDGSKKEYMAEIDIYGLNRSGLLNDVLQTL
                     SNATKLVSTVNAQPTKDMKFANIHVSFGISNLAQLTTVVDKIKIIPDVYSVKRTNG"
     gene            10251..10694
                     /locus_tag="VBC28_001202"
     CDS             10251..10694
                     /locus_tag="VBC28_001202"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HEO6815192.1"
                     /translation="MKIIIQRVNQASVSIEDDVVGSIEKGLVLLVGIAPEDTTEDIAY
                     AVRKITSMRIFSDDEGKMNLSIQDIKGSVLSISQFTLFADTKKGNRPAFTGAADPVKA
                     NQFYDIFNQELANHVSVETGQFGADMQVSLINDGPVTIVLDTKNK"
     gene            10949..11872
                     /locus_tag="VBC28_001203"
     CDS             10949..11872
                     /locus_tag="VBC28_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000822457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HEO6815193.1"
                     /translation="MKRIRKSLIFVLGVVTLICLCACTKQSQQKNGLSVVTSFYPVYS
                     ITKAVSGDLNDIKMIRSQSGIHGFEPSSSDVAAIYDADLFLYHSHTLEAWARRLEPSL
                     HHSKVSVIEASKGMTLDKVHGLEDVEAEKGVDESTLYDPHTWNDPVKVSEEAQLIATQ
                     LAKKDPKNAKVYQKNADQFSDKAMAIAEKYKPKFKAAKSKYFVTSHTAFSYLAKRYGL
                     TQLGIAGVSTEQEPSAKKLAEIQEFVKTYKVKTIFVEEGVSPKLAQAVASATRVKIAS
                     LSPLEAVPKNNKDYLENLETNLKVLVKSLNQ"
     gene            11899..14480
                     /locus_tag="VBC28_001204"
                     /pseudo
     CDS             11899..14480
                     /locus_tag="VBC28_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080375.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
     gene            complement(14762..15538)
                     /locus_tag="VBC28_001205"
     CDS             complement(14762..15538)
                     /locus_tag="VBC28_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000919193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEO6815194.1"
                     /translation="MLKRERLQKIIEKVNINGIVTVNEIMEELDVSDMTVRRDLDELD
                     KAGLLIRIHGGAQKVNASPTPQNYEKSNTEKYDIQTNEKLEIAQFAKQFINDGETIFI
                     GPGTTLEKLATQLLDFKIRVVTNSLPVFNILNQSSTLDLILVGGEYREITGAFVGSVT
                     INSIKSLNFSKAFVSSNGVFEKSIATYDEGEGEIQRIALNNSFEKFLLVDSQKFGKYD
                     FYTFYQLDDIDFVLTDHNIDNVVKEQYSSFTKILTNNNYM"
     gene            15748..16221
                     /locus_tag="VBC28_001206"
     CDS             15748..16221
                     /locus_tag="VBC28_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEO6815195.1"
                     /translation="MFAVTQDILFIDAHSQEELFDLVSKALIKQHYVSPNYRQAVKER
                     EREFPTGLKIDLKDGTPIQYVAIPHTETQYCLVDRIFYVKNSQPITFKHMINPEEECR
                     VQDFFFIINSRNSNQSDILSNLITFFITKGNLDRLHELGDNKEKINHYLIEKGVF"
     gene            16223..16528
                     /locus_tag="VBC28_001207"
     CDS             16223..16528
                     /locus_tag="VBC28_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEO6815196.1"
                     /translation="MIKILAACGAGVNSSHQIKDAIETQLGDRGYNVHCDAVMVKDIT
                     EEMVNKYDIFTPIAKTDLGFNVPIPVVEAGPILYRIPVMSEPVFTALEQVIKEHNLS"
     gene            16568..18016
                     /locus_tag="VBC28_001208"
     CDS             16568..18016
                     /locus_tag="VBC28_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001195826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HEO6815197.1"
                     /translation="MQVFLNIVNKFFDPIIHMGSGVVMLIVMTGLAMIFGVKFSKALE
                     GGIKLAIALTGIGAIIGILTGAFSESLQAFVKNTGINLSIIDVGWAPLATITWGSPYT
                     LYFLLIMLIVNIVMIVMKKTDTLDVDIFDIWHLSITGLLIMWYAKKNNLPTLLSVIIA
                     TVAIIFVGVLKIINSDLMKPTFDDLLGTGPTSPMTSTHMNYMMNPIIMVLDKLFDKVF
                     PGLDKYDFDAAKLNKAIGFWGSKFFIGMILGLVIGIMGNPVFSFAALGGWFSLGFTAG
                     ACLELFSLIGSWFIAAVEPLSQGITNFANGKMHGRRFNIGLDWPFIAGRAEIWACANI
                     LAPIMLVEAILLSKVGNGILPLAGIIAMGVTPALLVVTRGRLIRMITFGTLLLPLFLL
                     SGTMIAPFATELAKKVGAFPAGARAGSLITHSTLEGPMEKIFGYVIGKATTGQLSAII
                     TLIIFATAYLGLFMWYAKQMKRRNAEYAANQK"
BASE COUNT         5880 a         2953 c         3437 g         5797 t
ORIGIN      
        1 attaagccaa ttatagttaa taaaaaagtt tcaggttatg cctttttatt gatgaatggg
       61 aaagactttt tacttccaac gaaggctatt aactcagact tgattattgc cgatcagcta
      121 aataatagct ttacttttac taatcgtgat tttatctcct ctagtttaga taaggtagat
      181 agtcaattct taactagata ttttagtttt cacgatcatc gagcttttgt tgttaggaaa
      241 gtagctctgc aagacaatat cctactttac atgtatcgtc ccctgatacc agtaactctg
      301 gtcgtccttt tttctctagt atcatcagtt attatttttg tgattttacg acaaaaatct
      361 agagttctag ccgatcgcat cgcagtaaaa aattctagcg ccataaatca aatggtgcta
      421 gatatggatg ccatttcccg ccaagaaaaa tctagtattg agcttgatag tcaagatgaa
      481 tttcaatatc tatctgtgca aatcaatcag atggtttcac ggttaaaaga tttacatgag
      541 aaaactcttg atttggaaac acaaaagttg ttgtttgaaa agaggatgtt agaagctcag
      601 tttaatcctc acttccttta taataccctt gagacaattt taataaccag tcactatgat
      661 tcacaattaa cagaaagaat cgttatacaa ctaacaaaat tattgcgcta tagccttagt
      721 ggtagcacag aagcagctgt gcttaaggat gatttagcaa tcattgaatc ctaccttttg
      781 attaatcaag tgagatttga agaattaact tacactattt cagtatctcc tgaacttgaa
      841 cacatgcgtg taccaaaact tttcttgtta ccgctaattg agaatgctat taaatatggc
      901 ttaaaagaac gtcatgatgt agcaataaac atagatattt ggcaagatag tgatggaatt
      961 tggtttactg tttcaaataa tggatcaggt attagtttgg ctaggcaaca agccatacgt
     1021 acaatgttaa gatcaactca ctcgcatcat gggcttatta attcttatag acgattacaa
     1081 tatcaatttt caacagtatt gttagagttt acgaagacag atgatgcttt tcgagttagc
     1141 tatatagtaa aggagtgagt tatgtataga ttacttatcg ttgaggatga gcaccttatt
     1201 cgtaagtggt tacgctatgc tattgattat caatcattga atattttagt ggttggtgaa
     1261 gcaaaagatg gtaaagaagg agctcaatta attcaggaag agcagccaga cattgtttta
     1321 agtgatatta atatgccaat tatgacagca ttcgatatgt ttgaggcaac caaaggacaa
     1381 tcttatgcca aaattatttt atctggctat gctgattttc caaatgcgca atcagctatt
     1441 cactatggcg ttcttgaatt tttaacaaaa ccactagaaa agcaagcttt gattgattgt
     1501 ttaaaaacaa ttatggctag aattgaggaa cataaggaga agcacctaca agagcataca
     1561 gaattgtatt tacctctacc acaagctaat gaccaagttc ctgaagttat taaagatatg
     1621 ctagcatgga ttcatagcca ttttcatggg aaaattgtga ttagccagtt agctcacgat
     1681 ttaggttaca gtgaaagtta tctttacaca gtcactaaaa agcaccttca tataactttg
     1741 agtgattata taaatcaata tcgtattaat caagccattc agttgatgtt tagagaacca
     1801 gacctaatgg tttaccaaat tgcagaagct gttggtattt atgattatcg ttattttgat
     1861 agggttttta aaaagtactt gggtcagaca gtaaaagcgt ttaaggaaga acatattttt
     1921 aaacaaatgg attaggtagg tgaaagatga aagaaaaaca gtcgaaaagg cttatttata
     1981 tactactggt tgtttccatt atttttataa gtgtttttac atacagtatt agccagcctt
     2041 ctaaactact tccaccaaaa gaattagtta ttctaagtcc aaatagtcaa gccattttaa
     2101 caggaacgat tccagctttt gaggaaaaat acggtataaa agttaagctt attcaaggtg
     2161 ggacagggca actaatagat agattaagta aggagggtaa gcagttgaag gcggatattt
     2221 tctttggagg aaattatacg caatttgaaa gtcataaggc attgtttgag tcttacgtat
     2281 caaagaatgt tcatactgtt attccagact atatccatcc aagtgatacg gcgacacctt
     2341 atactataaa tgggagtgtc ttgattgtaa ataacgaatt agctaaggga cttaccatca
     2401 agagttatga agatttatta cagccttcct taaaaggtaa aattgccttt gcagatccga
     2461 atacttcctc tagtgctttc tcacaactca ctaatatact cttggccaag ggtggttaca
     2521 ccaatccaaa agcgtggaac tatgttaaaa agctacaaca taatattaat gctatcaaat
     2581 cttctagctc ttcagaagtt tatcaatcag ttgcagaagg aaaaatgatt gtggggctga
     2641 cttacgaaga ccctagtgtc aatttgcaaa aaagtggtgc caatgtttct attgtatatc
     2701 cgacagaagg gacagttttt gtcccatctt cggttgcaat tataaagaat gctccttcta
     2761 tgaaagaagc aaagttattt attaatttta tgctttcttt agatgttcaa aatgcctttg
     2821 ggcagtcaac gagtaaccga cctattcgta aagatgccca aacgagtaat ggcatgaaag
     2881 ctttaaagga tattgctact cttaaagaag attatcgcta tgtcactaag cataagggcc
     2941 aaatccttaa aacctataat cgtattcgta gaaatgctga ttaaatgaga atatagaaaa
     3001 actccttgga atcattgatt ccgaggagtt taatcgtcgt cttcgtcgct ttcgaaatct
     3061 tttttccaat aacgataaag actaatacat gccagaaatc cggctataat gggaacgata
     3121 ataaataaga ggtcaatcac tgtatccatg ggcaatatac tccaatgata agaagtcatt
     3181 attgtttata gtgcgtacaa taatgttaag gtaaaacgtc ggtcattata agttttttga
     3241 catcctctaa actcatagct tttgttaata ttttttcagg tatctctttt aagaaatctt
     3301 tctcaagcca ccatcttgca agagaatctt ctccaaattc taaagattta agtcgagttg
     3361 aatgtctttt aacagtttct tgaaagctta aatcatagta ataagcataa gtattgttgg
     3421 ggttatgttt taaaatatgt ttccaaacag gcgtatacca atcagaacga agtatgcctt
     3481 caaggatgat gtaggaacag ttatggtaac cataatttag taaattgatc aaaaggggaa
     3541 tggtagtagt attttcacca tccttagttc ctaacatctc tctacgaagg taatcctgag
     3601 ataagagaag ggtattttcc cctaattcgg cttgaagttg cttagctatt gtagactttc
     3661 cgctagctga atttcccctg ataataatga gcgtggctac cattagtcca ccatattgct
     3721 tttgagatag gcatccacta agagttcagt tgcttgaata gaatcgatat gggtacgttc
     3781 ataagaatga ctagattcaa tacctgcacc aagtagcgcg tgtttaactt ccgcaccagc
     3841 acgcatggca gcggaggcgt cactaccata ataaggataa atatcaagtt tataaggaat
     3901 attattgttt tcagctagag aaacaagatg ttgacgtaat tcataatgat aaggaccaga
     3961 agcatcttta acacagatag agacagtata ttcgtcagtt tcttgatcgt ctcccatagc
     4021 tcccatatca actgctagat attctacagt ttcatttggg atgcttgaat ttgctccatg
     4081 tcctagctct tcaaaggcac taaagtagaa atgagtagta taaggaagtt gaatgtcttc
     4141 tttcttgtaa acagaaagaa gttccattag gataccagcg gataccttgt catctaggta
     4201 acgtgattta ataaagccgc tgtctgttat gattgtacgc ggatcaaatg aaatgaaatc
     4261 accgacctgg atgcctaaag cacgtgtttc gtcagcagtt gttactttct catctaaacg
     4321 aatttccata tttgtttgat tacgttcagc agttccagcg tctttgtaaa catggacact
     4381 agtttgatga ataagagcag ttccagaaat ttctttacca ttttttgaga ggtgtatagt
     4441 acagttttct ccttcaatgg cattgtatgt atatccacca ataaggtcaa tttttaaccg
     4501 accatctggt ttgatggccc taaccatagc accaagtgta tcaaggtgag cagttatcat
     4561 gcgatgttta gtatcatttt ttccttttag agaaaccatg acgcctccct tgtttgtgcg
     4621 aattggtgag tagccaaacg cttccaattc tttgatgata taggtcatga ttgtttgggt
     4681 aaaccctgtt ggtgaaggga ttgatgtcag ggttttaatg tagtttaatg ttgtttccat
     4741 actatttcct ttaggcttta aagttcgata atgtttcaac gataatattt gcgatacgtt
     4801 caacgtcgct agaagttcct cgcaattcaa agtctcctaa cataggaaat ccaaaccgtt
     4861 cagagtattg ttttgcagtt aaacagtact gattgttaaa atttctattt ccagagccaa
     4921 caattccgat acaatttttg acattattgt gtgcagctat aaaatcacca agtggaatgg
     4981 taagaatttc ttgatcgcca ttgtcaatgc catttcctcc ctcaaggtaa gtgggtaata
     5041 gtgctacgaa aggttggtca acaggaaagg tttgatgatt ttgttccttg atattaatag
     5101 tcgaaacctg acagttatgc caagttgaca gttgttcact aagacgtttg acaaagcttt
     5161 gagtattgcc gctaagactg atatagacta gggttaattt aggcatttag tactccttaa
     5221 atctaaattt cttgttatca gtttagcaaa atcatgtcag ataaacaagc tagagtggta
     5281 tctcaatatc tgactattta gagagaaaat ctattgttga gactaaaaac tgaggttgat
     5341 tggcctcagt ttttagtgat gactatattt gtttcttttt aagtaaactt cccacgagtc
     5401 caagtattgc gacaccaaag aaagccagat aagattgttt ttcttgtcca gttttaggta
     5461 atgttttttc tttctgttgt gtatagacag tgcctgtaat atttggagaa gcttttgctt
     5521 ttgtataagc agttgcaacc atgtggtgaa ttttttgttt ttctatttgg cttaactttt
     5581 cttgatgtga tggctcagta aggtctactt tttcttgttc agtatagaca aatcgataat
     5641 caccaaatcc ttcatctttg gtagaactag cgatatactg aatatccttg ctattagtaa
     5701 caagattttt agctttatcg gctgtttgga atcgtaaatc aaggtcttta atagactctg
     5761 tgaagcccca gttattatca gctgtaggat tgatggtttt ctctgaaata atatagttga
     5821 taatagtttg gcgattttct aagttcagca aacggttcat tgtggcattt ttagccccag
     5881 ggaaggtacc actagcacgg tagttattgg tcacaatcat aaactcttgt ttgtctgcaa
     5941 caggcttacc attgtacatt aaatcgcgta ctcgactagc ttgactatta acaacttttc
     6001 catctttatt gtatttatta ggttgtgtaa tatcaaattt ataagtcaag ccgtcaataa
     6061 cgtcaaaatt ataggtacga tattcggtat tgataatatt ttgtggtgct ttattgttag
     6121 ggtcaatttg gttaaattga ccagcggaca tttctaacca ttcacgtaag tctgctccgg
     6181 tcacttttaa aagtgcagta acattatcat aaaggtaaag atcagctacg tttttaattg
     6241 caacaggtcc agctggaata tcagtatatg cggtagcatc gccgcgtgtt ccagctttaa
     6301 atggtgctgc agcagataga agtggtaaat ttgcctcagg tgttcctttc aattcatttg
     6361 caacgtacca acgttgagcg ttgttaacaa tttgaactga agggtcatct ttaactagtg
     6421 caaagtaact tgtaattggc gcagttgttg tacctacttg ttggcgaaca tagttgatag
     6481 tgccatcgtg tgcttcctta gccaatgcaa tgatacgctc gtcagcagtt gttgagttca
     6541 tatcaatttt acggatttta gcactacttt cggagacttg ccattttcca ttagtataac
     6601 taagtcctaa atcaataata ccaagatgat ccccgtactt gcctgccatt gtaacaggtg
     6661 ttccatttat ttttccattg ataccatcaa ctccagtgta tttttcatag aagccagtac
     6721 cgttacctga tggaaattca gcgtgtgagt gtcccgtaac aacggcatcc actcccttga
     6781 tgctggcaat ttgatagcca acgttttctt caccttcttc atatcgatca tctccaatgc
     6841 cagagtgtga taaaacgaga atgacatctg ctcctttagc acgcatagta ggaacgattt
     6901 cttcgattgc tttaactgaa tctttgacaa taacctttcc ctctaggtta gccttgtccc
     6961 agtttaagat ttgaggaggt acaattcctg taatcccaat atttagtgca acagctcttc
     7021 ctgttgaatc tttgaaggtt ttagtaatga tgtcataagt tttaaaaact ggttgatgtg
     7081 ttttgaaatc caaaacattt gcgttgagga ttggaaggtt ggctgtagca atgatttttt
     7141 tgaggtaatc taaaccgtaa ttaaactcat gattacctag tgttgatgca tcataaccaa
     7201 gagcattcat ggcttggtac ataggatgtt cttcattttc tgcgacaggt ttaacgattg
     7261 ctttataagt tcctaaaggt gtcccttgaa tgacatctcc gttatcaact aagacggtat
     7321 taggattttc tttttttgct tcttcaataa ggacagcagt ttttgctaac cctatttttt
     7381 gagattcttt atcttgataa taatcatagt taactaaatt ggtgtgtagg tctgtggttg
     7441 acatgatgcg aaggtcaact ttttcacctt caataggttt gagatcacta gttgtggctt
     7501 ctgtggcaac aggctgtgga ttttggttat tggtagtggt atttgtgctt gtggctgttg
     7561 aagcatcagt tattgcagtt ggagtagaag taacgtttga cttttcctct gcaattgtgc
     7621 tagtgggggt agttgattca acctttgtag aggttgaact tggtgttgta acaatgtctt
     7681 ctgcttgaac agctaaacca cttgttgctg tagctgttaa gacagttaaa gcgaatacgc
     7741 tttttgaaaa ataatgtttt gacataaatc tcctccatta aaacattaca tgtatattat
     7801 cctaatctaa aaactattat agcttattga aaacgtttaa tcaatctcat ttacgaatta
     7861 tttttagtga ttacaccaaa aacaatcgtt tatttataaa ggataactta gttagacaca
     7921 tttacttggt atttaattct ttttgagcct ctaacttctt ttatttttag gttaaaaagt
     7981 gataaaatag gtacaatcta tcactatgat taaagaggat aactatggtc aaagaaatta
     8041 atttaacagg agaagaggtt gttgcaatca catcccagta catgagtgag acggatgttg
     8101 cttttgtaaa atttgcctta aattacgcaa ccgcagctca ttattatcaa gcaagaaagt
     8161 cgggcgagcc ctacattatt catccaattc aagtagcagg tattcttgca gatttacacc
     8221 ttgatgccgt tacggtagct tgtggttttt tacatgatgt tgtagaagat acggaaatta
     8281 cccttgatga gattgaaact gattttggca aggatgttcg cgatattatt gacggcgtaa
     8341 caaaattggg taaagtggag tacaaatctc atgaagaaca gttagcagaa aatcaccgta
     8401 aaatgttaat ggctatgtct aaggatatcc gtgttatttt ggttaagctc gctgaccgct
     8461 tacataatat gagaaccctt aaacacctca gaaaagataa acaagagcgt atttcacgtg
     8521 agactatgga aatatacgct cctttagcgc atcgtttggg gattagtcgt atcaagtggg
     8581 aattagaaga tttatctttt cgttacttga atgagacgga attttataag atttctcata
     8641 tgatgagtga aaaacgtcgt gaacgcgaag agttggttga tataattgtc gataaaatca
     8701 gatcctatac tgaggaacag ggtttatatg gtgatattta tggtagacca aaacacattt
     8761 attctattta cagaaaaatg cgtgataaaa agaaacgctt tgatcagatt tatgatttaa
     8821 ttgcgatacg ctgtatcatg gaaactgcta gtgatgttta tgccatggtg gggtatatcc
     8881 atgagttatg gcgtcctatg ccaggaaggt ttaaagatta tattgcggca ccaaaggcca
     8941 atggttatca atctattcac acgacggttt atggacctaa agggccaatt gaaattcaga
     9001 ttcgaactaa agaaatgcat caagttgccg agtttggtgt tgcagcccat tgggcctata
     9061 aaaaaggaat caccagtaag gttaatcaag cagagcaatc ggttggtatg ggatggattc
     9121 aagaattagt tgagcttcaa gatgaatcaa aagacgccaa ggattttgtt gactctgtta
     9181 aggaagatat ctttacagaa cgcatctatg tttttacccc aaatggtgct gttcaggaat
     9241 tgccaaggga atctggacca attgattttg cttatgctat tcacacgcaa gttggagaaa
     9301 aagctactgg cgctaaagta aatggacgta tggttccatt gactgctaag ttaaaaacag
     9361 gagatgttgt tgagattatc acaaacccta attcatttgg accaagtcgt gactggatta
     9421 agattgttaa gaccaataaa gctcgcaata aaattcgtca gttcttcaaa aaccaagata
     9481 aagaaacttc tattaataaa ggtagagaat tactggttga ttatttccaa gaacaaggtt
     9541 atgtgcctaa caaatattta gataagaaac atattgaaga aatccttcca cgtgtcagtg
     9601 ttaaaagtga ggaagctttg tatgcagccg taggttttgg tgatttgagt ccgattagta
     9661 tttttaataa actgacggaa aaagaacgcc gtgaggagga acgtgctaag gctaaagcgg
     9721 aagcagacga acttattaac ggtggtgaaa tcaaaaccga taagcgtgat gttctcaaag
     9781 ttaagagtga aaatggtgtt atcattcaag gagcttcagg tttattaatg cgtattgcaa
     9841 aatgttgtaa tcccgttcct ggagatctca ttgaaggcta tataactaaa ggtagaggcg
     9901 tggctatcca tcgttcagat tgtcaaaatt taaagagtca ggagaactac gaacaacgtt
     9961 tgattgatgt tgagtgggat gatgatggat ctaaaaaaga gtacatggct gaaatcgata
    10021 tttatggttt gaatcgtagt ggcctcctta atgatgttct tcaaacctta tcaaatgcaa
    10081 caaagctagt atcgacagta aatgcacaac caacaaaaga tatgaaattt gctaatattc
    10141 atgttagctt tggaatttca aatttagcac aattgacaac tgtggttgat aaaatcaaaa
    10201 ttatcccaga tgtttattct gttaaacgta cgaatggtta ggagagcaaa atgaaaatca
    10261 ttattcaacg tgttaatcaa gcaagtgttt ctattgaaga tgatgtagtc ggatctattg
    10321 agaagggttt agtacttcta gttgggattg ctccagaaga tacaacagaa gatattgctt
    10381 atgcagttcg taaaattact agcatgcgta ttttttctga tgatgagggg aaaatgaacc
    10441 tttcaattca agacattaaa ggaagcgtct tatctatctc acaatttaca ctctttgcag
    10501 ataccaaaaa aggaaatcgt ccagctttta ctggggcagc tgatcctgtt aaagcgaatc
    10561 agttttatga tatctttaat caagaattag caaatcatgt ttcggtagaa acaggtcaat
    10621 ttggagctga tatgcaagtc agtctgatta atgatggacc agtaactatc gttttagata
    10681 ctaaaaataa ataaaaaacc aaagagttgg gcagaatgct tggctctttt tattgtggtt
    10741 ctaattatac tccttaaccc attcatgttt atgaaaagat ttactgtggc ttggaaaggc
    10801 atgaataaag gttgttgata tgaaattaag tgcacttaac aatattaaaa atagaaaaat
    10861 attagattta tggtttttcg ttgacactaa aataagaata ctctaaaatg ttaactggtt
    10921 aagtattttt tattttaaag gaggtttaat gaaaaggata cggaaaagcc ttatttttgt
    10981 tctcggagta gttaccctaa tttgcttatg tgcttgtact aaacaaagcc agcaaaaaaa
    11041 tggcttgtca gtagtgacta gcttttatcc agtatattcc attacaaaag cagtttctgg
    11101 tgatttgaat gatattaaaa tgattcgatc acagtcaggt attcatggtt ttgaaccctc
    11161 atcaagtgat gttgctgcca tttatgatgc tgatctattt ctttatcatt cgcacacact
    11221 agaagcttgg gcgagacgtt tggaacctag tttgcatcac tctaaagtat ctgtaattga
    11281 agcttcaaaa ggtatgactt tggataaagt tcatggctta gaagatgtag aggcagaaaa
    11341 aggagtagat gagtcaacct tgtatgaccc tcacacttgg aatgaccctg taaaagtatc
    11401 tgaggaagca caactcatcg ctacacaatt agctaaaaag gatcctaaaa acgctaaggt
    11461 ttatcaaaaa aatgctgatc aatttagtga caaggcaatg gctattgcag agaagtataa
    11521 gccaaaattt aaagctgcaa agtctaaata ctttgtgact tcacatacag cattctcata
    11581 cttagctaag cgatacggat tgactcagtt aggtattgca ggtgtctcaa ccgagcaaga
    11641 acctagtgct aaaaaattag ccgaaattca ggagtttgtg aaaacatata aggttaagac
    11701 tatttttgtt gaagaaggag tctcacctaa attagctcaa gcagtagctt cagctactcg
    11761 agttaaaatt gcaagtttaa gtcctttaga agcagttccc aaaaacaata aagattactt
    11821 agaaaatttg gaaactaatc ttaaggtact tgtcaaatcg ttaaatcaat aggtagttat
    11881 ataaagaaag gacgcaggat gaatcgtaaa aaaacagtta ttattagtgc tttatcagta
    11941 gctctatttg gtactggtgt aggtgcttac caattaggta gctataatgc ccaaaaatca
    12001 gacaatagtg tttcttatgt gaaaacagat aaatctgata gcaaagcaca agcaacagct
    12061 gtaaataaaa cgcccgacca aatcagtaaa gaagaaggca tatctgcaga acaaatcgtt
    12121 gttaaaatta ctgacgacgg ttatgtgact tctcatggag accattacca ttattacaat
    12181 gggaaagtgc catatgatgc catcattagt gaagaattga ttatgaaaga ccctagctat
    12241 gtctttaata aagctgatgt cattaatgaa gtcaaagatg gttatattat caaagttaac
    12301 ggtaagtatt acctctatct taaagaaggt agcaaacgaa caaatgttcg tacaaaggaa
    12361 caaatccaaa agcaacgcga agaatggtct aagggtggtt ctaagggaga atcaggaaag
    12421 catagtagtg caaaaactca agctttatca gccagcgtac gtgaagcgaa agctagcggt
    12481 cgctatacta cagatgatgg ttatgttttt agtccaacag atgtcattga tgacatggga
    12541 gatgcctttt tagtaccaca tggcgatcat ttccattata tcccgaaagc tgatttatct
    12601 ccatcagagt tatcagccgc acaggcatat tggaaccgca agactggccg atcaggtaat
    12661 agctcaaaac catcaaattc aagttcatac attcatgcaa gtgcaccaag cggtaatgta
    12721 tcaacaggaa gacatgctaa tgcccctatt tctattccaa gagtcactca cgcaaatcac
    12781 tggtcaaaac cagctggtaa tcatgctacg gcaccaaaac accatgcacc tacaaccaaa
    12841 ccaatcaaca aagattctgc tttagacaaa atgttgaaac gtttatatgc tcaaccgtta
    12901 tatgctcgtc acgtagaaag tgatggtttg gtttatgacc cagcacaagt taatgctttc
    12961 acagcaatcg gtgtttctat tccgcacggt aatcatttcc actttattca ctataaggat
    13021 atgtctccat tagagttaga agcaacaagg atggtggcag agcatagagg acatcatatt
    13081 gatgcattag ggaaaaaaga ttctacagag aaaccaaagc atatttctca tgaacctaat
    13141 aaggaacctc acacagagga agaacaccat gcagtaacac cgaaagacca acgtaaaggc
    13201 aaaccaaata gccagattgt ctacagtgct caagaaattg aagaggcaaa aaagctggta
    13261 aatacacaac atctgatggt tacatttttg atgccaaaga tattaaaaaa gatacaggta
    13321 caggttatgt cattccacat atgacacatg agcattgggt accaaagaaa gatttatcag
    13381 agtcggaatt aaaagcagct caagaatttc tttcaggaaa atctgaagca aatcaagaca
    13441 aaccaaaaac aggtaaaaca gctcaagaaa tctatgaggc aattgaacca aaagcaattg
    13501 ttaaacctga agatttatta tttggaattg cacaagcgac agactataag aatggtacat
    13561 ttgtaattcc tcataaagat cattaccatt atgtggaatt aaaatggttt gatgaagaaa
    13621 aagatctttt agctgattca gataagacat attctttaga agactattta gctacggcta
    13681 aatattacat gatgcaccca gaaaaacgtc ctaaagttga aggatggggt aaagatgctg
    13741 aaatttataa ggaaaaggac tctaataaag cagataaacc aagtcctgca ccaactgata
    13801 ataaatcaac atcaaattct agtgacaaaa acttaagtgc tgcagaagta ttcaaacaag
    13861 caaaaccaga aaaaattgta ccgcttgata aaattgctgc tcacatggca tatgcagttg
    13921 gatttgaaga tgatcaattg attgttcctc atcatgatca ttatcataat gttcctatgg
    13981 catggtttga caagggtggt ttatggaaag caccagaagg ctatacatta caacaactct
    14041 tctcaacaat taaatactac atggaacatc ctaatgaatt accaaaagaa aaaggttggg
    14101 gacacgacag tgatcataac aaaggctcaa ataaagacaa taaagccaaa aattatgctc
    14161 cagatgaaga acctgaagat tcagggaaag taactcacaa ctatggtttt tatgatgtta
    14221 ataaaggttc agacgaagaa gaaccagaaa aacaagaaga tgaatcagag ctagatgaat
    14281 atgaactagg aatggcacaa aacgctaaga aatatggtat ggatagacaa tcttttgaaa
    14341 agcaactcat ccaattatca aataaatata gtgtaagttt tgaaagcttt aattatatta
    14401 atggtagtca agttcaagtt actaaaaaag atggctctaa agtgcttgtt gatattaaaa
    14461 cactaacaga agtgaaataa taaaagagtt gagtaaactt agctgacaac taaaaacaaa
    14521 caagtcaccg tacttgtttg ttttttagtt atgatttcta gcgtgcgaag cttttaatga
    14581 tatatctaat tcttcttttg aacaaccatt gttttacgct taaaaagaga gttaatatat
    14641 ttttgtttga ggaagttttc ttgttttgac ttttataaaa cgactaaagt tataatcact
    14701 aaataaagag ccactactaa aagctgtata acaggtttct agtagtggct cacttgtttt
    14761 attacatgta attgttatta gttaaaattt tggtgaagct actgtattgc tccttaacaa
    14821 cattatcaat attatgatct gttaatacaa aatcaatatc gtctaattga taaaaagtat
    14881 aaaaatcata tttaccaaat ttttggctat caactaaaag gaatttttca aaggaattat
    14941 taagagcaat gcgctgaatt tcaccttctc cctcgtcgta agtagcgata gacttttcaa
    15001 aaacaccatt actactaacg aaagcttttg aaaaattaag tgatttaata gagttaatag
    15061 taacagaacc gacaaaagca ccagtaattt ctctgtactc tccccctaca aggataaggt
    15121 caagtgttga actttgattc agaatattga aaacaggaag gctattggtt actaccctaa
    15181 ttttaaaatc aagtaattgc gtggctaatt tctctaaggt tgttccaggc ccaataaaga
    15241 ttgtttcacc atcattgatg aattgttttg caaattgggc aatttctaat ttttcatttg
    15301 tttgaatatc gtatttttca gtgtttgatt tttcataatt ttgtggagtt ggtgatgcat
    15361 ttactttttg tgcaccacca tggatgcgta ttaacagtcc tgctttatct aattcatcca
    15421 agtcacgcct aacagtcatg tctgatacat caagttcttc catgatttca ttaacagtga
    15481 caatgccatt tatattgact ttttctatta ttttttgtaa tctttctcgc tttaacataa
    15541 tgacctctcc tacctcttcg ttatatatat cataccataa atagagctaa aataataata
    15601 attttgaaaa aataaacaat aataaacaaa ataaaataaa aaatgtcttg taatgtttta
    15661 ttttgttttg tataatattg atgtagataa gttgataagg cttgtttgcc ttacaacaaa
    15721 aatgtaaccg cttaactaag aagaaaggtg tttgctgtga ctcaagatat tttatttatt
    15781 gatgcccatt ctcaggaaga attatttgac ttagtatcaa aagcattgat aaaacaacac
    15841 tatgttagtc ctaactaccg acaggcagta aaagaaaggg agagagagtt tccaacgggg
    15901 ttaaaaattg atttaaaaga tgggactcct attcaatatg ttgctattcc acacactgaa
    15961 acgcagtatt gtctagtaga tagaatcttt tatgtcaaaa atagtcagcc aattactttc
    16021 aaacatatga ttaatccaga agaggagtgt agggttcagg attttttctt catcattaat
    16081 agtaggaata gtaaccaaag tgatatttta tctaatttga ttaccttctt tattacaaaa
    16141 ggaaacctag atcggctaca tgagcttggc gacaataaag aaaaaattaa tcattattta
    16201 atagaaaaag gagtgtttta acatgatcaa aatattagca gcttgtggtg caggcgtaaa
    16261 ctcaagtcat caaattaaag atgctatcga gacacaatta ggagatagag gttacaatgt
    16321 acattgtgat gctgttatgg ttaaggatat cactgaggaa atggtcaaca aatatgatat
    16381 ctttactcct attgctaaaa ctgatttagg ttttaatgta ccaattccag ttgtagaagc
    16441 aggacctatt ctttatcgca taccagttat gagcgaaccg gtctttactg ctttagaaca
    16501 agttatcaaa gaacacaatc tatcataaca tatttaaaat tggaattagt cttaaaggag
    16561 aaggaacatg caggtatttt taaatattgt caataaattc tttgatccaa ttatccatat
    16621 gggttcggga gttgtgatgc taattgtcat gacaggttta gccatgatat ttggagtgaa
    16681 gttttctaaa gcacttgaag gtggtattaa gttagctatt gctcttacgg gtattggtgc
    16741 tattattggt attttaactg gtgctttttc tgaatcactt caagcttttg ttaaaaatac
    16801 aggaatcaat ctaagcatta ttgacgttgg ttgggctcca ttagcaacta ttacatgggg
    16861 atcaccatat acgctttact tcttattaat catgcttatt gtcaatattg ttatgattgt
    16921 tatgaaaaaa acggatacct tagatgttga tatttttgat atttggcatt tatctatcac
    16981 aggtctttta attatgtggt atgctaaaaa aaataatcta ccaacacttt tatctgtcat
    17041 tattgcaact gtagctatta tcttcgttgg tgttctaaaa attatcaatt ctgatttgat
    17101 gaaacctacc tttgatgatt tattggggac aggtccaact tctcccatga cttcaacgca
    17161 tatgaattat atgatgaacc ccattattat ggttttggat aagctttttg ataaagtttt
    17221 tccagggcta gataaatatg actttgacgc ggctaaatta aataaagcca ttggattctg
    17281 gggctctaag ttctttattg gtatgatttt aggacttgtc atagggatta tgggcaaccc
    17341 cgtgttctca tttgcagcat taggtggctg gttttctctc ggttttacag caggtgcttg
    17401 tttagagttg ttctctttga ttggttcttg gttcattgct gcagtagaac cactttctca
    17461 aggaattact aattttgcta atggtaaaat gcatggtcgt cgtttcaata ttggtttgga
    17521 ctggccattt attgccggac gtgcagagat ttgggcatgt gctaacattc ttgcaccgat
    17581 tatgctagta gaagctattt tactctctaa agttggtaat ggtattctac cgcttgctgg
    17641 tattatcgca atgggtgtaa cccctgcttt actggttgtt acacgtggcc gtttaattcg
    17701 tatgattacc tttggaacat tgctattacc gctatttctt ttatcaggaa caatgattgc
    17761 cccatttgca actgaattag ctaaaaaggt aggcgcattc ccagctgggg cgcgagcagg
    17821 ttcacttatc actcactcta cactagaagg ccctatggag aaaatctttg gttacgttat
    17881 tggtaaagcg acaacaggtc aattatcagc gattataaca ttaattattt ttgcgacagc
    17941 ttatcttggt ttgtttatgt ggtatgctaa acaaatgaaa cgccgtaatg cagaatatgc
    18001 agctaatcaa aaataaatag aataaaaaaa cctaggagaa tgttatattt tctaggtttt
    18061 tgatata
//
LOCUS       DATRMA010000038        17770 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.038, whole genome shotgun sequence.
ACCESSION   DATRMA010000038 DATRMA010000000
VERSION     DATRMA010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17770
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.038"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            complement(<1..145)
                     /locus_tag="VBC28_001209"
     CDS             complement(<1..145)
                     /locus_tag="VBC28_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001876114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEO6815198.1"
                     /translation="MVSSDNTNLKFLKAFSELLKMRSFEQIKVSDLAKKARLSRRSFY
                     NHYN"
     gene            328..657
                     /locus_tag="VBC28_001210"
     CDS             328..657
                     /locus_tag="VBC28_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HEO6815199.1"
                     /translation="MLRKYKQRNYNVAHTKQRFSIKKFKFGVASVLIGLTFLGMSSHS
                     VLADESSIMSTEVPQTAGVKPEVTVTQPTSDVTSLATDTSTSAVLSTDASHSNPTESA
                     EHAVGLE"
     gene            complement(777..1921)
                     /locus_tag="VBC28_001211"
     CDS             complement(join(777..1687,1687..1921))
                     /locus_tag="VBC28_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612023.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISSag5 family transposase"
                     /protein_id="HEO6815200.1"
                     /translation="MSTFKRYDEEFKQSLVNLYQTGKTQSELCKDYRVSTSALAKWIK
                     QYSQFKLEDNSVLTAKQIQELQKRNAQLEEENLIPKKSKCHIHAKLKVRLLAVYRLRF
                     EHATTTLCRVLRVNRSTYYKFLKHKPSKRELDNQIYKKQILEIYTKANKRLGVKSIKV
                     ILQRDYDTKISEGRIYHLMKNMALPKMATVKPKTALKKTQKTYPQNLLNQKFYPDKPN
                     QVWSTDFTYISIGYKKYVYLCAILDLYSRKCIAWKLSHRMDAKLACNTLELALNKRKI
                     EGTLLFHSDQGSQFKAREFRKIIDDNNIMHSFSKPGYPYDNAVTEAFFKYLKHRQINR
                     KHYQNIKQVQLDCFEYIENFYNNYIPHTANLGLTPNQKEENYFNAIK"
     gene            2054..2191
                     /locus_tag="VBC28_001212"
     CDS             2054..2191
                     /locus_tag="VBC28_001212"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6815201.1"
                     /translation="MIIARENHQVTHEHVSILLTCVQELIVEVNQTQPLSREFREKYM
                     G"
     gene            2201..2456
                     /gene="mobC"
                     /locus_tag="VBC28_001213"
                     /pseudo
     CDS             2201..2456
                     /gene="mobC"
                     /locus_tag="VBC28_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263037.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
     gene            complement(2431..2925)
                     /locus_tag="VBC28_001214"
                     /pseudo
     CDS             complement(2431..2925)
                     /locus_tag="VBC28_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994071.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
     gene            complement(3119..4327)
                     /locus_tag="VBC28_001215"
     CDS             complement(3119..4327)
                     /locus_tag="VBC28_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000255843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HEO6815202.1"
                     /translation="MVTFDFKHVQTLHTISQLPISVMSRDKELVQLYGNEDYLLPYYQ
                     FLKHLAIPYNQDITVYEGLFEESFLIFPVCQYLIAIGPFYPYSPDINRQEQLSSRFLE
                     QFSHRSKKEILSYIKLVPCFPTTSIRSLLVSIDAFFQTQFEASCQQVINHLLEESEQI
                     VADPDIVLHLKHTKKNSFQLPTVLNHLNHIIDLVKLGNTQLLKQEINRLPSSSVTSSS
                     IPALRAEKNLTVVYLTKLLELSFEENTDVAKSYALVKHYMALNEEAPDLIDVSRIRCA
                     ALIDFSESLTNKSISDKQQMYNSILHYVDNHLYSKLKVSDIANYLYISDSHLRSVFKK
                     YSDISLQSYILKAKIKEGQLLLQRGVPIEEVAKLLHFYDTTHFLKTFKKYVGISSNEY
                     LTKYRETSCQ"
     gene            4708..6372
                     /locus_tag="VBC28_001216"
                     /pseudo
     CDS             4708..6372
                     /locus_tag="VBC28_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650896.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
     gene            6461..7384
                     /locus_tag="VBC28_001217"
     CDS             6461..7384
                     /locus_tag="VBC28_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEO6815203.1"
                     /translation="MKQTLKLMFSFLLMLGTMFGISQTVLAQETHQLTIVHLEARDID
                     RPNPQLEIAPKEGTPIEGVLYQLYQLKSTEDGDLLAHWNSLTITELKKQAQQVFEATT
                     NQQGKATFNQLPDGIYYGLAVKAGEKNRNVSAFLVDLSEDKVIYPKIIWSTGELDLLK
                     VGVDGDTKKPLAGVVFELYEKNGRTPIRVKNGVHSQDIDAAKHLETDSSGHIRISGLI
                     HGDYVLKEIETQSGYQIGQAETAVTIEKSKTVTVTIENKKVPTPKVPSRGGLIPKTGE
                     QQAMALVIIGGILIALALRLLSKHRKHQNKD"
     gene            7386..8303
                     /gene="srtC1"
                     /locus_tag="VBC28_001218"
     CDS             7386..8303
                     /gene="srtC1"
                     /locus_tag="VBC28_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase SrtC1"
                     /protein_id="HEO6815204.1"
                     /translation="MGQKSKISLATNIRIWIFRLIFLAGFLVLAFPIVSQVMYFQASH
                     ANINAFKEAVTKIDRVEINRRLELAYAYNASIAGAKTNGEYPALKDPYSAEQKQAGVV
                     EYARMLEVKEQIGHVIIPRINQDIPIYAGSAEENLQRGVGHLEGTSLPVGGESTHAVL
                     TAHRGLPTAKLFTNLDKVTVGDRFYIEHIGGKIAYQVDQIKVIAPDQLEDLYVIQGED
                     HVTLLTCTPYMINSHRLLVRGKRIPYVEKTVQKDSKTFRQQQYLTYAMWVVVGLILLS
                     LLIWFKKTKQKKRRKNEKAASQNSHNNSK"
     gene            8260..9111
                     /gene="srtC2"
                     /locus_tag="VBC28_001219"
     CDS             8260..9111
                     /gene="srtC2"
                     /locus_tag="VBC28_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase SrtC2"
                     /protein_id="HEO6815205.1"
                     /translation="MKKRLVKIVTIIRNNKIRTLIFVMGSLILLFPIVSQVSYYLASH
                     QNINQFKREVAKIDTNTVERRIALANAYNETLSRNPLLIDPFTSKQKEGLREYARMLE
                     VHEQIGHVAIPSIGVDIPIYAGTSETVLQKGSGHLEGTSLPVGGLSTHSVLTAHRGLP
                     TARLFTDLNKVKKGQIFYVTNIKETLAYKVVSIKVVDPTALSEVKIVNGKDYITLLTC
                     TPYMINSHRLLVKGERIPYDSTEAEKHKEQTVQDYRLSLVLKILLVLLIGLFIVIMMR
                     RWMQHRQ"
     gene            9193..11865
                     /locus_tag="VBC28_001220"
     CDS             9193..11865
                     /locus_tag="VBC28_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001868236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEO6815206.1"
                     /translation="MKKRQKIWRGLSVTLLILSQIPFGILVQGETQDTNQALGKVIVK
                     KTGDNATPLGKATFVLKNDNDKSETSHETVEGSGEATFENIKPGDYTLREETAPIGYK
                     KTDKTWKVKVADNGATIIEGMDADKAEKRKEVLNAQYPKSAIYEDTKENYPLVNVEGS
                     KVGEQYKALNPINGKDGRREIAEGWLSKKITGVNDLDKNKYKIELTVEGKTTVETKEL
                     NQPLDVVVLLDNSNSMNNERANNSQRALKAGEAVEKLIDKITSNKDNRVALVTYASTI
                     FDGTEATVSKGVADQNGKALNDSVSWDYHKTTFTATTHNYSYLNLTNDANEVNILKSR
                     IPKEAEHINGDRTLYQFGATFTQKALMKANEILETQSSNARKKLIFHVTDGVPTMSYA
                     INFNPYISTSYQNQFNSFLNKIPDRSGILQEDFIINGDDYQIVKGDGESFKLFSDRKV
                     PVTGGTTQAAYRVPQNQLSVMSNEGYAINSGYIYLYWRDYNWVYPFDPKTKKVSATKQ
                     IKTHGEPTTLYFNGNIRPKGYDIFTVGIGVNGDPGATPLEAEKFMQSISSKTENYTNV
                     DDTNKIYDELNKYFKTIVEEKHSIVDGNVTDPMGEMIEFQLKNGQSFTHDDYVLVGND
                     GSQLKNGVALGGPNSDGGILKDVTVTYDKTSQTIKINHLNLGSGQKVVLTYDVRLKDN
                     YISNKFYNTNNRTTLSPKSEKEPNTIRDFPIPKIRDVREFPVLTISNQKKMGEVEFIK
                     VNKDKHSESLLGAKFQLQIEKDFSGYKQFVPEGSDVTTKNDGKIYFKALQDGNYKLYE
                     ISSPDGYIEVKTKPVVTFTIQNGEVTNLKADPNANKNQIGYLEGNGKHLITNTPKRPP
                     GVFPKTGGIGTIVYILVGSTFMILTICSFRRKQL"
     gene            11919..12709
                     /locus_tag="VBC28_001221"
                     /pseudo
     CDS             11919..12709
                     /locus_tag="VBC28_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529916.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
     gene            12958..13563
                     /locus_tag="VBC28_001222"
     CDS             12958..13563
                     /locus_tag="VBC28_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prealbumin-like fold domain-containing protein"
                     /protein_id="HEO6815207.1"
                     /translation="MARDPYSDRSRYLDPKVLSSSFGAFFPADNIKVAWSNNSSTLFT
                     PPINANYTTQIQAIGTTIKSQIPESILTVTDKKSRKFSINKIDEAKEGLVGATFTLSK
                     RTTVAADHQVQGDFIPVSKETTVGRTTLTFDNLKPGFYDLKETKAPNAYVLDPKTYVV
                     VVQNSGKTTIVDEANFKEADYPMADNTSHVECVALLQRSKG"
     gene            14661..14837
                     /locus_tag="VBC28_001223"
     CDS             14661..14837
                     /locus_tag="VBC28_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000557948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6815208.1"
                     /translation="MHVGKSYFDYQENALIPQLGFLYSKLTKKIELDNRLSPTEQKLL
                     ITLLLHTKGLIIDM"
     gene            16182..16451
                     /locus_tag="VBC28_001224"
     CDS             16182..16451
                     /locus_tag="VBC28_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6815209.1"
                     /translation="MVSLLLLKVKQEELIEFHNVFYREAISSYLETENFEKAKVTEEN
                     YQVFLGIRTYQKSYNYQDHSENLKGNIEVAMIGVENNISDEHIFC"
     gene            16512..17663
                     /locus_tag="VBC28_001225"
     CDS             16512..17663
                     /locus_tag="VBC28_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEO6815210.1"
                     /translation="MVRKFLKSLLSLFLIAVIATGISVACFFFIPENKGNITPILLHR
                     FMRKNNVNGMMIVSDNTGKPITISHGINRGEVETDIENNKLFPMASLQKLMTGIIIQR
                     LIDQDVLSEDDRLSQFFPQVKGSNSITIHQLLTHTSGLREKGVKVSPYLKNEREQLQF
                     CLKHYNFVNKKSWYYSNINFSFLTGIATQVTGRTYAELVDDVIKNPLRLDDTQSYQSV
                     VNHDLVSPMRKNGKLNKINIFNQVSTAYGAGDFFTTPLNFWVLMRSFSKGYFFPTDEY
                     TKHQNDAISHYYGGLYMHGRIVNSNGTFFQYSSFGYADLKTHDTMVLFMNNKVYSDAS
                     HVAPKAFEYYQKFMFLNKIFHLVFYFVFSFFLMLLIRHSFRKRRYKKRL"
BASE COUNT         6021 a         2809 c         3500 g         5440 t
ORIGIN      
        1 aattgtaatg attatagaaa gatcttctac taagtcgagc tttttttgct aaatcgctaa
       61 ctttaatttg ctcaaagctc ctcatcttta acaattctga gaaagctttt aaaaatttta
      121 aattagtgtt atctgaactt accaatatac tatcaccttt atataaaatc ttaaagttat
      181 aaatcttatc gaatgtgatt atacaataca aattcaaata atagttcaca tttatcgaga
      241 taatgtaaaa cagtaaaact ttaaaaaaag taaaaataga taactttatt ggttaatcta
      301 tttttacttt tgaaaaggag agaagttatg cttaggaagt ataagcaaag aaattataat
      361 gtcgcacata cgaaacagcg tttttcgatt aagaaattca aatttggtgt agcatcggta
      421 ttaattggat tgacatttct aggaatgagt agtcattctg ttttggcgga tgagtcaagc
      481 attatgagta ctgaggtacc acaaacagct ggtgtaaaac cggaagttac tgttacacag
      541 cctacttcag atgtaacaag tcttgcgaca gatacgtcaa cttcagcagt gttatcaaca
      601 gatgcatctc attctaatcc tacagaatca gcggagcatg ctgtaggatt agaatgagat
      661 gcagcggttc cagtgacaca cgaaaaagaa gcaaataaaa cattgcgagt tacttcttct
      721 tccacatctg aattcacaga gtggactaat gtcaaataag tagacagaaa accgtgttat
      781 tttattgcgt taaaataatt ttcttctttc tgattagggg ttagtcctag attagccgta
      841 tgtgggatgt aattgttata aaaattctca atgtattcaa agcagtctaa ttgaacctgt
      901 ttgatatttt gataatgttt tcggttgatt tgtctatgct ttaaatactt gaaaaatgct
      961 tcagttacgg cattatcata aggatatcca ggtttagaaa aagaatgcat gatattgttg
     1021 tcatcaatta tttttctaaa ttccctggcc ttaaattgtg acccttggtc ggaatgaaag
     1081 agaagtgttc cttcaatctt tcttttatta agagctaatt ctagagtgtt acatgctaac
     1141 tttgcatcca tacggtgact caatttccaa gcaatacatt ttctagaata gagatcaagt
     1201 attgcgcaga gatagacata tttcttatat ccaatagaaa tataggtgaa gtcagtagac
     1261 catacttgat taggtttgtc aggataaaat ttctggttga gtaagttttg aggatacgtt
     1321 ttttgagtct ttttaagagc tgtttttggc ttaacggtag ccattttagg gagcgccata
     1381 ttcttcatca gatggtaaat tcttccttca gagatttttg tgtcgtagtc tctttgaaga
     1441 atgactttga tagacttcac accaagtctt ttgtttgctt tggtataaat ctcaagtatt
     1501 tgttttttat aaatttgatt atccaattct ctttttgagg gcttatgttt tagaaattta
     1561 tagtaagtgg agcgattgac acgtaaaaca cgacacaagg ttgttgtggc gtgttcaaag
     1621 cgtaaccgat agacagcgag gagtcttact ttaagttttg catgaatatg gcacttgctt
     1681 tttttaggat aaggttctcc tcttcaagct gcgcattacg tttttgtaac tcttgtattt
     1741 gcttggcagt aagtacagaa ttatcttcga gtttgaattg agagtactgt ttgatccatt
     1801 ttgcaagcgc agaagtggat accctatagt ctttacagag ttcagactga gtttttccag
     1861 tttgataaag gttaacaaga gattgtttga attcttcatc atagcgttta aatgttgaca
     1921 taggttgtcc tttcgttttt gtgtctttta aaacagattg taacacacaa agttttgtct
     1981 acttttatag tatacatcca ccctttggca ataccaaaag tttgagcaga ttagttgaga
     2041 tgtgcaggaa gttttgatta ttgcaagaga gaatcatcaa gtgactcatg aacacgtgtc
     2101 aatcttattg acctgcgttc aagaattgat tgtggaagtc aatcaaacac agccactcag
     2161 tcgtgagttt cgtgaaaagt atatgggtta ggaggctgca attgtttatc gttatcgtac
     2221 caatctcaaa aaagtctttc taacagattc agaattgaat caattaaatg agcggattgc
     2281 taagagcaat tgtcaaaatt tctcagtcta tgctagaaga gttcttctta atcccaatat
     2341 gtcatttgtc acgattaaca cggattggaa ataacatcaa tcaaattgcg cgtgctatca
     2401 accaaagcca tctaatttct caggctcagt atcactttca tcgagaagca cgttaagccc
     2461 aacagcaggg gggttgctct gattcggtta agtaataagc aaaatcctca ccaattttcc
     2521 cagtaataag aggcgttgtt gaggtatagg gattacctgt gccatagtcg atgatggtca
     2581 caaaatgacc attccccata aaagggccaa ccaaaacttc acctgtgacc gttttttgat
     2641 gtcaacacca gtattttgga tgtacccctt aacatgccct ttggtatctg caacagagat
     2701 aatatgacca aaggaagaat caccgatgac tttaaccgta atcttgctat cgcctttttg
     2761 attagccgct aatatttgat tagcaatcag ggtgcgacca agagcaactg ttgaagacga
     2821 caaggtatga tgtttttcct gagccaattt aacagtctcg gtgctatcga ggacataaga
     2881 cctgaaggca ccagaggcag aaattgattt aataatttta tccataacct atgttatagc
     2941 acaaagagag agtttgtatg gactggatta gctgaaaatt ttagactaaa aagtagtgcc
     3001 agtggatttt gaaaggtaac aaaagagtct tccgtttaaa cgagaaggct ctttttagtc
     3061 gtttcagatg aaggcaaaag gacgatgaaa taattccgta ccttcatgga ttgctatgtt
     3121 actggcatga ggtctcacga tatttagtaa gatattcgtt tgaagatatt cccacgtatt
     3181 ttttaaaggt tttaagaaaa tgtgtggtgt cgtaaaaatg taataatttc gctacttcct
     3241 caatcggtac ccctctttgc agtagtaatt gtccctcctt aatttttgcc tttagaatat
     3301 aactttgcaa ggaaatgtca gagtattttt taaaaactga gcgtaagtgg gaatctgaga
     3361 tatataggta gttggcaata tcagatactt tgagtttgga gtagaggtgg ttgtccacat
     3421 aatggagaat actattgtac atttgctgct tgtcagagat gctcttattg gttaaggatt
     3481 ctgaaaaatc aataagagct gcacagcgaa ttcttgaaac atcaataaga tcaggagcct
     3541 cttcgtttaa agccatatag tgctttacca gcgcataact tttagccaca tcagtatttt
     3601 cctcgaaact taattctagt aattttgtta agtaaacaac agttaagttc ttttcagctc
     3661 ttagggcagg gattgaagat gaggtaacac tggatgatgg gaggcgatta atttcttgct
     3721 ttaacagttg agtgttaccc agcttaacga gatcaataat gtgattgaga tggtttaaaa
     3781 cagtgggtaa ctgaaaagag tttttcttag tatgttttag gtgaagaaca atatcaggat
     3841 ccgcaacaat ctgttctgac tcttctaata aatgattgat gacttgttgg caactagcct
     3901 caaactgtgt ttggaaaaag gcatcgatag acacaagaag actacgtata ctggtagtag
     3961 gaaaacaagg gacaagcttt atataggata agatttcttt tttactacga tgagaaaatt
     4021 gttctagaaa gcgactggat aactgttctt gcctattgat atcagggcta tagggataaa
     4081 atggtccaat agcaataaga tattgacaga caggaaaaat taagaatgat tcttcaaaaa
     4141 gaccctcata aacagtgata tcttggttat aagggatagc taaatgtttt aaaaactgat
     4201 agtaaggcaa cagatagtct tcgttaccat ataactgaac gagttccttg tctcgtgaca
     4261 tgactgaaat aggtagttga gatatggtat gcaatgtttg aacatgttta aaatcgaatg
     4321 taaccatttg atagaccgcc ttcattatca tttctagaat ttttctttag gtttgtaaag
     4381 actacaaaat aaaatgatga aaacaactat cttgtggata cactaaaaag acacgctaat
     4441 tagcaaactc tctcttcatc atctctcacc attattatac tactatttat atgacaaata
     4501 aaggtgattt tgttaaaata taactttgaa aatccacata tatttttaat cttccgtctg
     4561 aaaaaataaa taaaaatagt aaaaataaac acgaatttaa aataagcaaa ttttttaaga
     4621 aaatctgtgc taaactttaa tagttttgtg cttaataata atcagcactt acaaagaaca
     4681 aagggaaaag cgaggagaga acttttaatg aaattatcga agaagttatt gttttcggct
     4741 gctgttttaa caatggtggc ggggtcaact gttgaaccag tagctcagtt tgcgactgga
     4801 atgagtattg taagagctgc agaagtgtca caagaacgcc cagcgaaaac aacagtaaat
     4861 atctataaat tacaagctga tagttataaa tcggaaatta cttctaatgg tggtatcgag
     4921 aataaagacg gcgaagtaat atctaactat gctaaacttg gtgacaatgt aaaaggtttg
     4981 caaggtgtac agtttaaacg ttataaagtc aagacggata tttctgttga tgaattgaaa
     5041 aaattgacaa cagttgaagc agcagatgca aaagttggaa cgattcttga agaaggtgtc
     5101 agtctacctc aaaaaactaa tgctcaaggt ttggtcgtcg atgctctgga ttcaaaaagt
     5161 aatgtgagat acttgtatgt agaagattta aagaattcac cttcaaacat taccaaagct
     5221 tatgctgtac cgtttgtgtt ggaattacca gttgctaact ctacaggtac aggtttcctt
     5281 tctgaaatta atatttaccc taaaaacgtt gtaactgatg aaccaaaaac agataaagat
     5341 gttaaaaaat taggtcagga cgatgcaggt tatacgattg gtgaagaatt caaatggttc
     5401 ttgaaatcta caatccctgc caatttaggt gactatgaaa aatttgaaat tactgataaa
     5461 tttgcagatg gcttgactta taaatctgtt ggaaaaatca agattggttc gaaaacactg
     5521 aatagagatg agcactacac tattgatgaa ccaacagttg ataaccaaaa tacattaaaa
     5581 attacgttta aaccagagaa atttaaagaa attgctgagc tacttaaagg aatgaccctt
     5641 gttaaaaatc aagatgctct tgataaagct actgcaaata cagatgatgc ggcatttttg
     5701 gaaattccag ttgcatcaac tattaatgaa aaagcagttt taggaaaagc aattgaaaat
     5761 acttttgaac ttcaatatga ccatacctcc tgataaagct gacaatccaa aaccatctaa
     5821 tcctccaaga aaaccagaag ttcatactgg tgggaaacga tttgtaaaga aagactcaac
     5881 agaaacacaa acactaggtg gtgctgagtt tgatttgttg gcttctgatg ggacagcagt
     5941 aaaatgacag atgctcttat taaagcgaat actaataaaa actatattgc tggagaagct
     6001 gttactgggc aaccaatcaa attgaaatca catacagacg gtacgtttga gattaaaggt
     6061 ttggcttatg cagttgatgc gaatgcagag ggtacagcag taacttacaa attaaaagaa
     6121 acaaaagcac cagaaggtta tgtaatccct gataaagaaa tcgagtttac agtatcacaa
     6181 acatcttata atacaaaacc aactgacatc acggttgata gtgctgatgc aacacctgat
     6241 acaattaaaa acaacaaacg tccttcaatc cctaatactg gtggtattgg tacggctatc
     6301 tttgtcgcta tcggtgctgc ggtgatggct tttgctgtta aggggatgaa gcgtcgtaca
     6361 aaagataact aaataaaagg ctacttctta agtaaccatg tttaagaaaa gagaaatagc
     6421 cttatttctc tttttgtcgt ttttaaaata aaggaacatc atgaaacaaa cattaaaact
     6481 tatgttttct tttctgttga tgttagggac tatgtttgga attagccaaa ctgttttagc
     6541 gcaagaaact catcagttga cgattgttca tcttgaagca agggatattg atcgtccaaa
     6601 tccacagttg gagattgccc ctaaagaagg gactccaatt gaaggagtac tctatcagtt
     6661 gtaccaatta aaatcaactg aagatggcga tttgttggca cattggaatt ccctaactat
     6721 cacagaattg aaaaaacagg cgcagcaggt ttttgaagcc actactaatc aacaaggaaa
     6781 ggctacattt aaccaactac cagatggaat ttattatggt ctggcggtta aagccggtga
     6841 aaaaaatcgt aatgtctcag ctttcttggt tgacttgtct gaggataaag tgatttatcc
     6901 taaaatcatc tggtccacag gtgagttgga cttgcttaaa gttggtgtgg atggtgatac
     6961 caaaaaacca ctagcaggcg ttgtctttga actttatgaa aagaatggta ggactcctat
     7021 tcgtgtgaaa aatggggtgc attctcaaga tattgacgct gcaaaacatt tagaaacaga
     7081 ttcatcaggg catatcagaa tttccgggct catccatggg gactatgtct taaaagaaat
     7141 cgagacacag tcaggatatc agatcggaca ggcagagact gctgtgacta ttgaaaaatc
     7201 aaaaacagta acagtaacga ttgaaaataa aaaagttccg acacctaaag tgccatctcg
     7261 aggaggtctt attcccaaaa caggtgagca acaggcaatg gcacttgtaa ttattggtgg
     7321 tattttaatt gctttagcct tacgattact atcaaaacat cggaaacatc aaaataagga
     7381 ttagcatggg acaaaaatca aaaatatctc tagctacgaa tattcgtata tggatttttc
     7441 gtttaatttt cttagcgggt ttccttgttt tggcatttcc catcgttagt caggtcatgt
     7501 actttcaagc ctctcacgcc aatattaatg cttttaaaga agctgttacc aagattgacc
     7561 gggtggagat taatcggcgt ttagaacttg cttatgctta taacgccagt atagcaggtg
     7621 ccaaaactaa tggcgaatat ccagcgctta aagaccccta ctctgctgaa caaaagcagg
     7681 caggggtcgt tgagtacgcc cgcatgcttg aagtcaaaga acaaataggt catgtgatta
     7741 ttccaagaat taatcaggat atccctattt acgctggctc tgctgaagaa aatcttcaga
     7801 ggggcgttgg acatttagag gggaccagtc ttccagtcgg tggtgagtca actcatgccg
     7861 ttctaactgc ccatcgaggg ctaccaacgg ccaagctatt taccaattta gacaaggtaa
     7921 cagtaggtga ccgtttttac attgaacaca tcggcggaaa gattgcttat caggtagacc
     7981 aaatcaaagt tatcgcccct gatcagttag aggatttgta cgtgattcaa ggagaagatc
     8041 acgtcaccct attaacttgc acaccttata tgataaatag tcatcgcctc ctcgttcgag
     8101 gcaagcgaat tccttatgtg gaaaaaacag tgcagaaaga ttcaaagacc ttcaggcaac
     8161 aacaatacct aacctatgct atgtgggtag tcgttggact tatcttgctg tcgcttctca
     8221 tttggtttaa aaagacgaaa cagaaaaagc ggagaaagaa tgaaaaagcg gctagtcaaa
     8281 atagtcacaa taattcgaaa taataaaatc agaaccctca tttttgtgat gggaagtctg
     8341 attctcttat ttccgattgt gagccaggta agttactacc ttgcttcgca tcaaaatatt
     8401 aatcaattta agcgggaagt cgctaagatt gatactaata cggttgaacg acgcatcgct
     8461 ttagctaatg cttacaatga gacgttatca aggaatccct tgcttataga cccttttacc
     8521 agtaagcaaa aagaaggttt gagagagtat gctcgtatgc ttgaagttca tgagcaaata
     8581 ggtcatgtgg caatcccaag tattggggtt gatattccaa tttatgctgg aacatccgaa
     8641 actgtgcttc agaaaggtag tgggcatttg gagggaacca gtcttccagt gggaggtttg
     8701 tcaacccatt cagtactaac tgcccaccgt ggcttgccaa cagctaggct atttaccgac
     8761 ttaaataaag ttaaaaaagg ccagattttc tatgtgacga acatcaagga aacacttgcc
     8821 tacaaagtcg tgtctatcaa agttgtggat ccaacagctt taagtgaggt taagattgtc
     8881 aatggtaagg attatataac cttgctgact tgcacacctt acatgatcaa tagtcatcgt
     8941 ctcttggtaa aaggagagcg tattccttat gattctaccg aggcggaaaa gcacaaagaa
     9001 caaaccgtac aagattatcg tttgtcacta gtgttgaaga tactactagt attattaatt
     9061 ggactcttca tcgtgataat gatgagaaga tggatgcaac atcgtcaata acgatgttgt
     9121 gaatggctta cttacttatc aaataggtga ctaatgatga ttgtgaataa tggttatcta
     9181 gaagggagaa aaatgaaaaa gagacaaaaa atatggagag ggttatcagt tactttacta
     9241 atcctgtccc aaattccatt tggtatattg gtacaaggtg aaacccaaga taccaatcaa
     9301 gcacttggaa aagtaattgt taaaaaaacg ggagacaatg ctacaccatt aggcaaagcg
     9361 acttttgtgt taaaaaatga caatgataag tcagaaacaa gtcacgaaac ggtagagggt
     9421 tctggagaag caacctttga aaacataaaa cctggagact acacattaag agaagaaaca
     9481 gcaccaattg gttataaaaa aactgataaa acctggaaag ttaaagttgc agataacgga
     9541 gcaacaataa tcgagggtat ggatgcagat aaagcagaga aacgaaaaga agttttgaat
     9601 gcccaatatc caaaatcagc tatttatgag gatacaaaag aaaattaccc attagttaat
     9661 gtagagggtt ccaaagttgg tgaacaatac aaagcattga atccaataaa tggaaaagat
     9721 ggtcgaagag agattgctga aggttggtta tcaaaaaaaa ttacaggggt caatgatctc
     9781 gataagaata aatataaaat tgaattaact gttgagggta aaaccactgt tgaaacgaaa
     9841 gaacttaatc aaccactaga tgtcgttgtg ctattagata attcaaatag tatgaataat
     9901 gaaagagcca ataattctca aagagcatta aaagctgggg aagcagttga aaagctgatt
     9961 gataaaatta catcaaataa agacaataga gtagctcttg tgacatatgc ctcaaccatt
    10021 tttgatggta ctgaagcgac cgtatcaaag ggagttgccg atcaaaatgg taaagcgctg
    10081 aatgatagtg tatcatggga ttatcataaa actactttta cagcaactac acataattac
    10141 agttatttaa atttaacaaa tgatgctaac gaagttaata ttctaaagtc aagaattcca
    10201 aaggaagcgg agcatataaa tggggatcgc acgctctatc aatttggtgc gacatttact
    10261 caaaaagctc taatgaaagc aaatgaaatt ttagagacac aaagttctaa tgctagaaaa
    10321 aaacttattt ttcacgtaac tgatggtgtc cctacgatgt cttatgccat aaattttaat
    10381 ccttatatat caacatctta ccaaaaccag tttaattctt ttttaaataa aataccagat
    10441 agaagtggta ttctccaaga ggattttata atcaatggtg atgattatca aatagtaaaa
    10501 ggagatggag agagttttaa actgttttcg gatagaaaag ttcctgttac tggaggaacg
    10561 acacaagcag cttatcgagt accgcaaaat caactctctg taatgagtaa tgagggatat
    10621 gcaattaata gtggatatat ttatctctat tggagagatt acaactgggt ctatccattt
    10681 gatcctaaga caaagaaagt ttctgcaacg aaacaaatca aaactcatgg tgagccaaca
    10741 acattatact ttaatggaaa tataagacct aaaggttatg acatttttac tgttgggatt
    10801 ggtgtaaacg gagatcctgg tgcaactcct cttgaagctg agaaatttat gcaatcaata
    10861 tcaagtaaaa cagaaaatta tactaatgtt gatgatacaa ataaaattta tgatgagcta
    10921 aataaatact ttaaaacaat tgttgaggaa aaacattcta ttgttgatgg aaatgtgact
    10981 gatcctatgg gagagatgat tgaattccaa ttaaaaaatg gtcaaagttt tacacatgat
    11041 gattacgttt tggttggaaa tgatggcagt caattaaaaa atggtgtggc tcttggtgga
    11101 ccaaacagtg atgggggaat tttaaaagat gttacagtga cttatgataa gacatctcaa
    11161 accatcaaaa tcaatcattt gaacttagga agtggacaaa aagtagttct tacctatgat
    11221 gtacgtttaa aagataacta tataagtaac aaattttaca atacaaataa tcgtacaacg
    11281 ctaagtccga agagtgaaaa agaaccaaat actattcgtg atttcccaat tcccaaaatt
    11341 cgtgatgttc gtgagtttcc ggtactaacc atcagtaatc agaagaaaat gggtgaggtt
    11401 gaatttatta aagttaataa agacaaacat tcagaatcgc ttttgggagc taagtttcaa
    11461 cttcagatag aaaaagattt ttctgggtat aagcaatttg ttccagaggg aagtgatgtt
    11521 acaacaaaga atgatggtaa aatttatttt aaagcacttc aagatggtaa ctataaatta
    11581 tatgaaattt caagtccaga tggctatata gaggttaaaa cgaaacctgt tgtgacattt
    11641 acaattcaaa atggagaagt tacgaacctg aaagcagatc caaatgctaa taaaaatcaa
    11701 atcgggtatc ttgaaggaaa tggtaaacat cttattacca acactcccaa acgcccacca
    11761 ggtgtttttc ctaaaacagg gggaattggt acaattgtct atatattagt tggttctact
    11821 tttatgatac ttaccatttg ttctttccgt cgtaaacaat tgtaaggtgt cgttgaaatt
    11881 attaaataat agaaaatgac tagttttggc ctttccctat tgtcagtcag attagttatt
    11941 atcaagcttc tcatgccaat attaatgcct ttaaaagaac agttacaacc attgaccgta
    12001 cggagattca acgacggtta ggtctggcta aagcctacaa tgctagtatt tctggaacaa
    12061 gtagtcagtc gactcaatct gtgctgagag attcttattc tgaggagcaa aaaaggcagg
    12121 gtggactgaa tacgctagga tgttagaagt cagagagcag gttgaccatg tgatgattcc
    12181 aaaaatcaat caggatttac caatctacgc tggttcagaa gaggacaatc tgcaacgggg
    12241 agttggtcat ctagaaggga taagtttgcc gattggaggg gcttctacac atgcggtctt
    12301 gagcggtcaa agaggtatgc cagctgctcg gttgtttgcg gatttggata agatgaaaaa
    12361 aggtgattat ttttatgtta ccaatctgaa agaaaccttg gcttatcaag tggatcgtat
    12421 catggtgatt gaacctagcc aattggatgc cgtgagcatt gaagaggata aagattatgt
    12481 tacccttctg acctgtacac cttatatggg ctctttgtca actgtaatgg gtgacttatc
    12541 attaacaacg agagagaatc agcttggttc tctctctttt tggatgttca aagcgatgag
    12601 aattttgctt ttaaaatttt taaagttgcg aaagccaaag gcttgtcgct tgatgtcttt
    12661 gataagcttg ttggtcgctt ctaatttggc attggaaagg gatagctgag ggcgtttgtg
    12721 atgtaagttt tgtacttcat gaaggtccta aagaccattt taaaagcaga attgccagtt
    12781 gttacgttat catctatgaa gctaaagaat tcagttgttc gcttttctta gaaatgaaag
    12841 agtatgagct gataaaggtc gttataatag cggagctcat ctgagaaaac ttttatacct
    12901 caaagtcagt ctagctttga tatcatgaag ccattaggag ttattcctta tcttttagtg
    12961 gcgcgcgatc catatagtga tagatcgaga tatttagatc caaaagttct atcatcctct
    13021 tttggcgcct tttttccagc agataatatt aaggtagctt ggtctaacaa ctccagcact
    13081 ttatttacac cacctattaa tgcaaactac accactcaga ttcaagctat tgggacaacg
    13141 attaagtcac aaattccgga atcgattttg acggttacgg ataaaaagag caggaagttc
    13201 agcattaaca agattgacga agctaaagaa ggcttagtag gtgcgacctt caccttgtct
    13261 aaacgcacaa cagtagcggc agatcatcaa gtacaaggag atttcattcc tgtcagcaaa
    13321 gagacgacag tcggtcggac aacccttacc tttgacaacc ttaaacctgg attttatgac
    13381 cttaaagaaa cgaaagcgcc gaatgcttac gtacttgatc ctaagactta tgttgtggtc
    13441 gttcaaaatt caggaaaaac gacaattgtg gatgaagcta acttcaaaga ggctgattac
    13501 ccaatggctg ataataccag ccatgtggag tgcgtagcgt tgctacaacg aagcaaaggg
    13561 taaaaatcct ttattttaag cactttttca agcattttgt ctttattgaa aagagtgatt
    13621 ttaacataaa aaaggtatta aaaaacatat tgacgtgacc gtttgttttg aagtggcttg
    13681 cgtagacaaa aaaatagata cgtcagataa atttctggca ttacgagaac atttttagag
    13741 tgttctcttt ttttagttta cggaggaaaa atatatatgg aaaaacagga ttcacgagtt
    13801 ctcatccatt gggaggggaa ttctggggac aagctcattg aacaccaaac cagcgcaacg
    13861 gggtggtact accaagtcga tcgtagcttt agtcaaccaa aaggggaacc gcccagaatg
    13921 atccaaaggc actagaaagt gtccgtaatg attcgatttc gggcggtgat gatgtcatgg
    13981 gttatgctta tagcaaatgt acttggggag ttgcggcacg aattaatcag tgggactgaa
    14041 actcaaaggt tgaaatggtg agaagattac cattaccagt tcaatgggaa atggtcagga
    14101 ttgggttgga acagccgaaa gactagatgg tgaaactgat acagttccaa aagaaggtac
    14161 tattctctct ttttaggaaa gtagttatgg ttcgtatata ggctacggaa ctatatcttt
    14221 cgtcacatta catctacaga tagtaccatg aattttgctt atatgaccaa gtaaagtgag
    14281 gatatactaa caaatgaaat atttattatc gtatttgtcc attttatcga aaagtttgca
    14341 tattatcatt atgtttgata agatgcaaat ataatgatag taggagctaa atatggatat
    14401 ttaaaaaatc aagagtatcc taagtgcttt ccattttgaa attcaaatat agctaatagt
    14461 tctagaactt ctaattgttt ttcgtcgacg atatgaattt tcaatcttaa ctgttaggat
    14521 tccacctccc tttggttaaa gaaaaaaggt caggtcgttt agataacttt gtcaaacaag
    14581 ctcaagctat ctaaaaatag tttgaaatgg gcattactct agtttttaat aagctatctg
    14641 atgagcagaa ggagaagtta atgcatgttg ggaagtctta ttttgactat caagaaaatg
    14701 ctcttatccc acaattaggt tttctatatt ctaaattaac taaaaaaatt gaacttgata
    14761 atcggttgtc tccgactgaa caaaagttat tgattacctt attattacat actaaaggtt
    14821 taatcattga tatgtaagaa gtaagtcagc taaccgatct ttctattcta aaacttatat
    14881 tgttgcttta gaaattttaa agagcgtgga tggcttcata ataaacagaa atcttaccaa
    14941 tttgcgaagc caaaaaatat gatatttgaa gagtccaaag atctaataga tagtccagtt
    15001 agagaagcgt tgattataag tgataaggat tttcaaaaat taaaacaaga gctattattt
    15061 taaccgactt attttaaaga cttatcatat ctaggcttgc ttgatgattc ggaaaaatac
    15121 ggagactata ctatttcaag gaaaagatac aaaagtttcg aatcaagtct tcaactatac
    15181 atccttcaaa gtcatcggct agagatttgg aattatgaac caatcccttt gattactaga
    15241 aaaataaata gcttggagag gctaacgtga cactggttga tccaatctcg ctttatttaa
    15301 cactaagaat gatgaagacc ctcgtattga agaagaagtt gagcagctag aagataagat
    15361 tttgagaata taatcgtttt ttcgtgctac actaacttgt cagggttgca acagacaaaa
    15421 aaataattta ggagggattc gttatgcgta caaagtatat aggatatgaa attgttttaa
    15481 ggggtggtta ttaatatgga taagaaaaag gttgtttcaa ctatcgtatc tacagggttt
    15541 cttatagttt caatatcaaa tttttttaga gagctatcac ctatactttt tctcttgaat
    15601 attattggat ttttctgctt cctattatta ttttctgcca acataaataa attgttctta
    15661 aaacgatagc cgttatatag gatatggtat gtatgcattt ggtgaaatta taggtaaaac
    15721 gaatggaata gtacttactc ctcaaagcta tcgtgagagg tataaaagga tatcgaggag
    15781 tcactagata gttggatctc attgaaaaaa tttgtttaaa gtatctcata taagcataaa
    15841 acgaggaatc aatcaaaatt tcttgattga ttccttgttt tttggctctt tgtcaactgt
    15901 agtgggttga tgaaaaatta tattctggag aaaaccaaat tggttttctc ctttttgata
    15961 ttcaaagtga tatgaattct ctttttaaaa tattcaaagt ttctaaagct aaatgtttgt
    16021 gcttgatgat tttgatgaga ttattggttg ctcaagtttg gtgtttgaat aaggcgattc
    16081 aaggacattg ataaccttat ctctgtcttt aaaaaatgtc ctaaaaacag tttaaaagat
    16141 tggagtgaca ctagagaaag tgttttaaac agggtaaaga aatggtgagc ttgcttctct
    16201 tgaaagtaaa gcaggaggag ttgatagagt ttcataatgt tttttataga gaagcgatat
    16261 cttcttatct tgaaacagaa aattttgaga aggctaaagt gactgaagag aattaccaag
    16321 tttttttagg aatccgtact tatcagaaga gttataacta tcaagatcat agtgagaatc
    16381 taaaaggaaa tattgaggta gcaatgatag gagttgaaaa taatattagc gacgaacata
    16441 ttttttgcta gaatatataa aatgtctttg aaataattta gccctgtgtt atgatattaa
    16501 ggaggttatt tatggtacgt aaatttttaa aatcgctcct atctctcttt cttatagcag
    16561 taatcgccac cggtatttca gtggcatgtt tcttttttat tccagaaaat aaagggaata
    16621 ttacaccgat tttattgcat cgctttatgc gaaaaaataa tgttaatgga atgatgattg
    16681 ttagcgataa tactggtaaa ccgattacca tctcgcatgg gattaaccgt ggggaagttg
    16741 aaacggatat tgaaaataat aaattatttc caatggcttc tctccaaaaa ctcatgacag
    16801 gaattattat ccaaagatta attgatcaag atgtcttgag tgaagacgac cgattgtctc
    16861 agttctttcc ccaagtaaaa ggtagtaata gtattaccat ccaccaattg ttgacccata
    16921 cgagtgggtt aagagaaaaa ggtgttaagg tgtctcctta tctaaaaaat gagagagaac
    16981 aattacaatt ttgcttaaaa cactataact ttgttaataa gaaatcgtgg tattattcta
    17041 atatcaactt tagtttccta acaggtatag ccactcaagt tacgggaagg acctacgctg
    17101 aacttgttga tgatgtgata aaaaatcctt tgcgattaga tgatacccaa agttaccaat
    17161 cagttgttaa tcatgactta gttagcccaa tgcgaaagaa cggcaagtta aataaaatta
    17221 atatctttaa tcaagtatcc acagcatatg gagctggaga ttttttcaca actccgctga
    17281 acttttgggt gttgatgcgg tctttttcta aagggtactt tttcccaacg gatgaatata
    17341 ccaaacatca aaatgatgcg atatcacatt attatggtgg gctttacatg catggtcgta
    17401 tagttaattc taatgggacc tttttccagt attcaagttt tggttatgca gatctaaaga
    17461 cgcatgatac aatggtgctt tttatgaata acaaagtcta tagtgatgct agtcacgtgg
    17521 caccaaaagc atttgaatat tatcaaaaat ttatgttcct taataagatt ttccacttag
    17581 tcttttactt tgtattttct ttctttttaa tgcttttaat acgtcattcg tttaggaaaa
    17641 gaaggtataa gaaacgtctc taaatcacat gaataaaaaa gaaggctaag ttggccttct
    17701 tttttattca atattaagca gtaacaccat ctaagaaaac gtttgcattg aatttttaat
    17761 tgtgctataa
//
LOCUS       DATRMA010000039        16552 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.039, whole genome shotgun sequence.
ACCESSION   DATRMA010000039 DATRMA010000000
VERSION     DATRMA010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16552
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.039"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            complement(105..764)
                     /locus_tag="VBC28_001226"
     CDS             complement(105..764)
                     /locus_tag="VBC28_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001934124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEO6815211.1"
                     /translation="MPVKDFMTKKLVYVSPDTTVAEAADLLREHHLRRLPVVENDQLV
                     GLVTEGTMAEAQPSKATSLSIYEMNYLLNKTKIRDIMIKDIVTVSQYASLEDAIYLMM
                     SRKIGVLPVVDNGQLYGIVTDRDVFKAFLEIAGYGQESYRLVILADEGIGVLSKVLNR
                     LSSANLSVKRLVIIERKAGKKAVEIQLEGYADKDVLKQELVFDDVIVETLEKTMKKPL
                     S"
     gene            complement(783..1493)
                     /locus_tag="VBC28_001227"
     CDS             complement(783..1493)
                     /locus_tag="VBC28_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6815212.1"
                     /translation="MTMLKVENLSIHYGVIQAVNDVSFEVNQGEVVTLIGANGAGKTS
                     ILRTISGLVRPSQGSISFMGKPIHKLAARKIVGNGLAQVPEGRHVFSSLSVMENLEMG
                     AFLQKDREQNQKMLKKVFDRFPRLEERKNQDAATLSGGEQQMLAMGRALMSRPKLLLL
                     DEPSMGLAPIFIQEIFNIIEDIKKQGTTVLLVEQNANKALTIADKAYVLETGKVVLSG
                     TGKELLVSDQVRKAYLGG"
     gene            complement(1493..2257)
                     /locus_tag="VBC28_001228"
     CDS             complement(1493..2257)
                     /locus_tag="VBC28_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6815213.1"
                     /translation="MALLEVKNLSKHFGGLTAVGDVSMKLHKGELIGLIGPNGAGKTT
                     LFNLLTGVYLPSKGTISIDGKILNGRKPAKIASLGLGRTFQNIRLFKNMTVLDNVLVG
                     LSNHHLSHPIASFLRLPKYYHSEKALRKKALELLEIFGLKAYQDALAKNLPYGKQRRL
                     EIVRALATEPKILFLDEPAAGMNPQETAELTQLISQIKSDFDITIMLIEHDMNLVMQV
                     TERIYVLEYGRLIAHGTPEEIKNNKRVIEAYLGGEL"
     gene            complement(2258..3211)
                     /locus_tag="VBC28_001229"
     CDS             complement(2258..3211)
                     /locus_tag="VBC28_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000662325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEO6815214.1"
                     /translation="MKENMKSILSWLAIVVLDYLLISVLISMGIFNLYHIQIIETIGI
                     NVILAVGLNLIVGCSGQFSLGHAGFMAIGAYAVAIIGVKMPTYVGFLIAILVGTLVAG
                     GIALGVGIPTLRLKGDYLAIATLGVAEIIRILLVNGGDITNGAAGIMGIPPFTTWSLV
                     YGVAVVSLILAMNFLRSPLGRNTIAIREDEIAAESMGVDTTKVKVIVFVFGAILASIA
                     GSLQASYVGTVMPKDFSFMMSVNVLIIVVLGGLGSMTGTVLAAILLGLLNMLLQDYAS
                     VRMIIYALALILIMIFRPSGLLGTKELTLSHLFRKTSKGGN"
     gene            complement(3214..4083)
                     /locus_tag="VBC28_001230"
     CDS             complement(3214..4083)
                     /locus_tag="VBC28_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000941401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEO6815215.1"
                     /translation="MLQQLVNGLILGSIYALLALGYTMVYGIIKLINFAHGDIYMMGA
                     FMGYYLINHLHLNFFLALLIAMLGSAFLGVVIEYLAYRPLRKSTRIAALITAIGVSFL
                     LEYGMVYLVGADTRAFPQAIHTVKYNLGPITITNVQLIILGIALLLMLTLQFIVQKTK
                     MGKAMRALSVDSDAAQLMGINVNRTISFTFALGSALAGAGGVLIGLYYNSVQPLMGVT
                     PGLKAFVAAVLGGIGIIPGAAIGGFVIGILETLATALGVSDFRDGIVYAILILIFLIR
                     PAGILGKNIKEKV"
     gene            complement(4189..5355)
                     /locus_tag="VBC28_001231"
     CDS             complement(4189..5355)
                     /locus_tag="VBC28_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEO6815216.1"
                     /translation="MEKRLSLGALVLASTVLLAACGNVGGGASSTGTKIGKDIKVGYN
                     WELSGNVSSYGNSMKNGADLAVKEINAAGGVGGKKLKVLSQDNKSENAEAATVATNLV
                     TKGANVIIGPATSGAAASSTPKVNAAAVPMIAPAATQDNLVYGSDGKTLNQYFFRATF
                     VDNYQGKLLSQYATDNLKAKKVVLFYDNSSDYSKGVAKSFKESYSGKIVDSMTFSAGD
                     TDFQASLTKLKGKEYDAIVMPGYYTETGLIVKQARDLGISKPVLGPDGFDSPKFVQSA
                     TPVGASNVYYLTGFTTQGSTKAKAFHDHYVKAYGEEPSMFSALSYDAVYMAAKSAKGA
                     KTSIDIKKALAKLKDFKGVTGKMSIDKNHNVVKSAYVVKLEDGKTSSVNIISAK"
     gene            complement(5510..5755)
                     /locus_tag="VBC28_001232"
     CDS             complement(5510..5755)
                     /locus_tag="VBC28_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2129 domain-containing protein"
                     /protein_id="HEO6815217.1"
                     /translation="MNKQERLGMSVYLYYNRDARKLYKYGDVHYHSRRLRYLVIYVNK
                     EDIVSVSKEIKHLKFVKDVRLSAIDDIDQDFVGNLYR"
     gene            complement(5855..6445)
                     /locus_tag="VBC28_001233"
     CDS             complement(5855..6445)
                     /locus_tag="VBC28_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HEO6815218.1"
                     /translation="MIPVVIEQTSRGERSYDIYSRLLKDRIIMLTGQVEDNMANSIIA
                     QLLFLDAQDNTKDIYLYVNTPGGSVSAGLAIVDTMNFIKSDVQTIVMGMAASMGTIIA
                     SSGAKGKRFMLPNAEYMIHQPMGGTGGGTQQSDMAIAAEHLLKTRHTLEKILADNSGQ
                     SIEKVHDDAERDRWMSAQETLDYGFIDAIMENNNLQ"
     gene            complement(6570..7199)
                     /gene="upp"
                     /locus_tag="VBC28_001234"
     CDS             complement(6570..7199)
                     /gene="upp"
                     /locus_tag="VBC28_001234"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002883819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEO6815219.1"
                     /translation="MGKFQVISHPLIQHKLSILRRTTTSTKDFRELVDEIAMLMGYEV
                     SRDLPLEDVEIQTPVATTVQKQLAGKKLAIVPILRAGIGMVDGFLSLVPAAKVGHIGM
                     YRDEETFQPVEYLVKLPEDIDQRQIFVVDPMLATGGSAILAVDSLKKRGAASIKFVCL
                     VAAPEGVAALQEAHPDVDIYTAALDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(7309..8478)
                     /locus_tag="VBC28_001235"
     CDS             complement(7309..8478)
                     /locus_tag="VBC28_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEO6815220.1"
                     /translation="MITYDFTSLPERFSSNTIKWKAVQKDQEILPLWIADMDFPIFPE
                     MSEAIEDFSHQMVFGYDSPKDSLYQAISNWEVQEHGYQFDKKSLLLIDGVVPAISVAI
                     QAFTKEGDAVLINTPVYPPFARTIKYNNRHLVSNSLLNNNQYFEIDFKQLEKDIIENN
                     VKLYIFCSPHNPGGRVWTKGEIQKIGDICKRYNVILVSDEIHQDLVLFDNVHHSFNTV
                     DSSFKELSVILSSATKTFNIAGTKNSFAIIENEKLRSDFKKRQIANNQQEISSLGLLA
                     TEVAFTKEKQWLKALKMELEGSIEYLYEQLTQKTHIKVMKPEGTYLVWLDFSAYNLTH
                     LEIQEKLRYDAKLILNDGLTFGKEGKKHARINVAAPRSVIEEAVLRLVTNFRKND"
     gene            complement(8614..9162)
                     /locus_tag="VBC28_001236"
     CDS             complement(8614..9162)
                     /locus_tag="VBC28_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001019793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HEO6815221.1"
                     /translation="MNIETLTQKNHRSDSGRNHIVLFEPQIPANTGNIARTCAATNAP
                     LHIIRPMGFPIDDKKMKRAGLDYWDKLDVSFYDGLEEFMLSCRGKVHLISKFADKVYS
                     DENYNDDQDHYFMFGREDKGLPETFMREHAEKALRIPMNDEHVRSLNVSNTVCMIVYE
                     ALRQQSFPNLELSHTYENDKLK"
     gene            complement(9335..10687)
                     /locus_tag="VBC28_001237"
     CDS             complement(9335..10687)
                     /locus_tag="VBC28_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEO6815222.1"
                     /translation="MENHNSIKQTYGLMTTIAMIVGVVIGSGIYFKVDDILKFTGGDV
                     FLGMVILVLGSFSIVFGSLSISELAIRTSESGGIFSYYEKYVSPALAATLGLFASFLY
                     LPTLTAIVSWVAAFYTLGESSSLESQIILAAVYILALSLMNIFAKRIAGGFQSLTTFV
                     KMIPLVLIALIGAFWSDKAPQLPQHLTAIQPSNVGWSWVSGLVPLYFAYDGWTIFVNI
                     APEVKNPKKNLPLAFVIGPALILLSYLAFFYGLTQILGASFIMTTGNDAINYAANIIF
                     GPSVGRLLSFIVILSVLGVANGLLLGTMRLPQAFAERGWIKSERMANINLKYQMSLPA
                     SLTVTAVAIFWLFVHFMVTKFNLLPGSDISEIAVVFNNTSLIILYVLVLSLYLKKDIK
                     NKFTGLVSPILAILGGLILLIGSLLSNFFTVLIFQCFCLLFCLICHYIYQKNNPKTHE
                     "
     gene            10893..12242
                     /gene="trkA"
                     /locus_tag="VBC28_001238"
     CDS             10893..12242
                     /gene="trkA"
                     /locus_tag="VBC28_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HEO6815223.1"
                     /translation="MRIIVVGGGKVGTALCRSLVAEKHDVVLIEKKENVLKRVTKQHD
                     IMGIVGNGANYKILEQAEVKNCDIFIAITDRDEVNMISAVLAKKMGAKETVVRMRNPE
                     YSNPYFKDKNFLGFSSVVNPELLAAQYIANTIEFPNATSVEHFANGRVMLMEFKILEG
                     NKLCHTSMSQIRKKFGNIVICAIERDGKLIIPDGDATIQVKDKIFVTGNRIEMILFHN
                     YVKNKVVKNLMVIGAGRIAYYLLNILKNTNTHVKLVELNQEQAEYFSQEFPNVPVVHG
                     DGTAKNILLEESVTSFDAVATLTGVDEENIITSMFLESIGIPKNITKVNRTSLLEIID
                     DKQLSSIITPKRIAVDHVMHFVRGRVNAQDSNLEAMHHIANDRIETLQFEIKETSKLA
                     NRSLASLKLKQNILIAAIIRNNKTIFPTGEDVLTVGDRIVVITLLKNITRTSDMLAR"
     gene            12247..13686
                     /locus_tag="VBC28_001239"
     CDS             12247..13686
                     /locus_tag="VBC28_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEO6815224.1"
                     /translation="MNKSMIRFLLSKLLLIEAALLAIPLTVGLIYREPQSVMMSIVIT
                     MIILIILGLLGSLFKPKNYHIYTKEGMLIVALCWILWSFFGALPFVISGQIPNIIDAF
                     FEVSSGFTTTGATILDDVSVLSPALLFWRSFTHLIGGMGVLVFALAIMENSKNSHLEV
                     MRAEVPGPVFGKVVSKLKKTAQILYLLYLLMFAVFAVILYFAGMPFFDSIIIAMGTAG
                     TGGFAVYNDSIAHYNSPLITNLVSIGMLIFGVNFNLYYLLLLRKIKAFFGDEELKTYL
                     RIVAIATFMIALNVIGMYDNFRQGLEHIFFEVSAIITTTGFGVTDITRWPLFSQVILL
                     FLMFIGGSAGSTAGGFKVMRSLILAKIARNQVLSTLYPNRVMSLHINKSVLDKNTQHG
                     VLKYLTIYLAIFMALVLVLTLDTNDFLVVISAAASCFNNIGPLLGSNETFSFFSPFSK
                     LLLSFAMIAGRLEIYPVLLMFIPKTWSKL"
     gene            complement(13710..13961)
                     /gene="yidD"
                     /locus_tag="VBC28_001240"
     CDS             complement(13710..13961)
                     /gene="yidD"
                     /locus_tag="VBC28_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEO6815225.1"
                     /translation="MLKSFLIFLVRFYQKNISPAFPASCRYRPTCSTYMIEAIQKHGL
                     KGVLMGIARILRCHPLAHGGNDPVPDHFSLRRNKTDISD"
     gene            complement(13961..14683)
                     /locus_tag="VBC28_001241"
     CDS             complement(13961..14683)
                     /locus_tag="VBC28_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001934129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEO6815226.1"
                     /translation="MRINKYIAHAGIASRRKAEELIKQGMVTINGQVVNELATQVKAG
                     DLVEIEGSPIYNEEKVYYLLNKPRGVISSVSDDKGRKTVIDLLPQVKERIYPVGRLDW
                     DTTGLLILTNDGDFTDKMIHPRNEIDKVYLARVKGIATKENLRPLTRGVVIDGKKTKP
                     ARYTIIKVDHEKNRSVVELTIHEGRNHQVKKMFEQVGLLVDKLSRTQFGTLDLTGLRP
                     GEARRLNKKEISQLHNAAINKS"
     gene            complement(14673..15257)
                     /locus_tag="VBC28_001242"
     CDS             complement(14673..15257)
                     /locus_tag="VBC28_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein B"
                     /protein_id="HEO6815227.1"
                     /translation="MTYLGSIEALLFVAGEDGLSLRQMAELLSLTPSALIQQLEKLAK
                     RYEEDDDSSLLLLETAQTYKLVTKDSYMTLLRDYAKAPINQSLSRASLEVLSIIAYKQ
                     PITRIEIDDIRGVNSSGAITRLIAFGLIKEAGKKEVLGRPNLYETTNYFLDYMGINQL
                     DDLIDASSIELVDEEVSLFSMDSINTEDKENNEN"
     gene            complement(15254..15961)
                     /locus_tag="VBC28_001243"
     CDS             complement(15254..15961)
                     /locus_tag="VBC28_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEO6815228.1"
                     /translation="MDIKLKDFEGPLDLLLHLVSKYEVDIYDVPIVEVIEQYLAYIAT
                     LQAMRLEVAGEYMLMASQLMLIKSRNLLPKVVESNPIEDDPEMELLSQLEEYRRFKVL
                     SEELANQHQERAKYFSKPKQEVIFEDAILLHDKSVMDLFLTFSQMMSQKQKELSNSQT
                     VIEKEDYRIEDMMIVIERHFNLKKKTTLQEVFADCQTKSEMITLFLAMLELIKLHQIT
                     VEQDSNFSQVILRKEEK"
     gene            complement(15961..>16552)
                     /gene="xerD"
                     /locus_tag="VBC28_001244"
     CDS             complement(15961..>16552)
                     /gene="xerD"
                     /locus_tag="VBC28_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017770859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEO6815229.1"
                     /translation="EFKLTARKRKLSAVNQFLFFLYNRGTLKEFYRLQETEKITLAQT
                     KSQIMDLSNFYQDTDYPSGRLIALLILSLGLTPAEIANLKKADFDTTFNILSIEKSQM
                     KRILKLPEDLLPFLLESLEEDGDLVFEHNGKPYSRQWFFNQLTDFLNEKNEQQLTAQL
                     LREQFILKQKENGKTMTELSRLLGLKTPITLERYYR"
BASE COUNT         5344 a         3299 c         2525 g         5384 t
ORIGIN      
        1 aacttagtct gagacggatt ttcgttggtt tttattggtt tttatcagac taaattccat
       61 ttctactaga ggtggaactt actttgagaa tttagctgat atttttaaga aagtggtttt
      121 ttcatggtct tttctaaagt ttccacaata acatcatcaa atactagttc ttgttttaaa
      181 acatccttgt cagcgtatcc ttctagttgt atctcgaccg ctttcttacc tgcttttcgt
      241 tcaataatga ctaaacgttt aacacttaaa tttgcactag aaagacgatt taggactttt
      301 gatagtaccc ctatcccctc atcagctaat ataaccaagc gataactttc ttgaccatac
      361 cccgcaattt caaggaaggc tttaaaaaca tccctatccg tgacaatccc ataaagttgt
      421 ccattatcaa ctactggtaa aacaccgatc tttcgagaca tcatcaaata aatagcgtcc
      481 tcaaggctag catactgtga aacagtcaca atatctttaa tcataatatc tcgaattttt
      541 gtcttattaa gaagatagtt catctcatat atagaaagac tggttgcttt agaaggttgt
      601 gcttctgcca ttgtcccttc tgtcactaac cccactagct gatcattttc aacaactggc
      661 aaacgcctta agtggtgttc tctcaataaa tcagcagctt ctgcaactgt agtatctgga
      721 gagacataaa ctaatttttt tgtcataaaa tctttcactg gcatacctga ctcctctaaa
      781 tattatccac ctaagtaagc tttacgaact tgatctgaaa ctagtaattc cttacctgtc
      841 cctgataaaa caaccttccc agtctctaga acatatgctt tatcagcaat cgtcagagct
      901 ttatttgcat tttgttcaac taaaagaact gtagtccctt gttttttgat gtcttcaatg
      961 atattaaaaa tttcttgaat aaaaattgga gctaggccca ttgacggttc atctaaaagt
     1021 aataatttag gacgactcat caatgcacgc cccatagcga gcatttgctg ctcaccacct
     1081 gataaggttg ctgcatcttg gtttttacgt tcttctaagc gaggaaaacg atcaaacact
     1141 ttttttaaca ttttttgatt ttgttcacga tccttttgaa gaaaggctcc catttctaaa
     1201 ttttccataa cagacaaact tgagaaaacg tggcgccctt ctggaacctg agctaaacca
     1261 ttgccaacaa tttttctagc tgctaactta tgtataggtt tccccataaa agaaatagaa
     1321 ccctgacttg gtcttactaa accagaaatt gtcctcaaaa ttgatgtctt tcctgcacca
     1381 ttggctccaa ttaaggttac aacctcacct tggttaactt caaacgagac atcattcacc
     1441 gcttgaataa caccatagtg aatagataga ttttctactt tcaacatggt cattatagct
     1501 caccccccaa gtatgcttct ataacacgtt tattattttt aatttcttca ggtgtcccgt
     1561 gagcaatcag acgaccatat tccaatacat aaattctctc tgttacttgc ataaccaagt
     1621 tcatatcatg ttcaatcagc ataatagtaa tatcaaaatc actctttatt tgactaatta
     1681 actgcgtcaa ttctgcagtt tcttgaggat tcattcctgc cgcgggctca tccaaaaaca
     1741 atatttttgg ttctgttgct aaagcacgaa caatttctaa acgccgttgt tttccataag
     1801 ggaggttctt agcaagagca tcttgatatg ctttcaaacc aaaaatctct aatagctcca
     1861 gtgccttttt ccttaaggct ttttcactat gatagtattt gggtaatcgt aaaaaactag
     1921 caataggatg tgataagtga tgattactga gtcctactag tacattgtct aaaacagtca
     1981 tatttttaaa aagtcggata ttttgaaaag ttctacctaa acctaaagaa gctattttag
     2041 ctggtttcct cccatttaat atcttaccat caattgatat tgtcccttta ctaggaaggt
     2101 atactcctgt taagagatta aaaagcgttg tcttacccgc accgttgggg ccgattaagc
     2161 caattaattc acctttatgt aatttcatcg aaacatcacc tacagcagtt aggccaccga
     2221 aatgtttact taaatttttt acttcaagaa gtgccatcta attgcctccc ttactcgtct
     2281 tacgaaatag atgagataga gttaactctt tcgttcctaa taaaccgcta ggtctaaaaa
     2341 tcataattaa aatcagggct aaagcataga taatcattcg aacagaagcg tagtcttgta
     2401 gtaacatatt caataatcct aataaaattg ctgctaaaac tgtccctgtc attgaaccta
     2461 aaccacctaa aacaacaata attaaaacat tgacagacat cataaatgaa aagtcttttg
     2521 gcataacagt tccaacgtag ctagcctgta aactaccagc gatagaagct aaaatagctc
     2581 caaaaacaaa aacaattact ttcacttttg ttgtatccac acccatagac tctgctgcaa
     2641 tttcatcttc tcgtatagca attgtattac gccctaaggg actacgcaaa aaattcattg
     2701 caaggataag gctcacaact gccaccccat aaactaaaga ccaggttgta aaaggcggaa
     2761 tccccatgat acccgcagca ccattcgtaa tatcacctcc attaaccaac aaaatacgga
     2821 taatctcagc aacacctaaa gtagctatag ctaaataatc accttttagt ctcaaagtag
     2881 gaattcctac acctaaggca atacctcctg caacaagagt tccaacaagt atagctatca
     2941 aaaaacctac ataagttggc attttaacgc cgataatagc aactgcataa gcgccaatag
     3001 ccatgaaacc tgcatgtcct agtgaaaact gtcccgaaca acctacaatt aggttcaaac
     3061 caactgctaa aataacatta atcccaattg tttcaataat ttgaatatga tagaggttaa
     3121 atatacccat agatattagc acgctaatca agagataatc taacactact atagctaacc
     3181 aagataaaat tgatttcata ttttctttca tgcctagacc ttctccttaa tattttttcc
     3241 aagaattcct gctggacgga tcaagaaaat gagaattaaa atagcataaa cgataccatc
     3301 tcgaaaatct gacacaccaa gtgcagtcgc taaagtttct aaaatgccaa taacaaaacc
     3361 accgatagcc gctcccggaa tgataccaat accacctaag acagctgcaa caaatgcttt
     3421 caaccctgga gttaccccca taagaggttg aacagaatta taataaagcc caatcagtac
     3481 tccacctgct ccagctaaag cagaccccag cgcaaatgta aaactaatgg tacggttaac
     3541 attaattccc atcaattgtg cggcatcgct atccactgat aaagctctca tagctttccc
     3601 cattttagtt ttttgaacga tgaactgtaa agtaagcatt agtaatagcg caatacctaa
     3661 aatgatgagc tgaacattgg ttatagttat cggtcctaaa ttatatttaa cagtatgaat
     3721 ggcttgcgga aatgccctag tatccgctcc aacaaggtat accatcccgt actccaaaag
     3781 aaaagaaaca ccaatagctg taattaacgc ggcaattcta gttgattttc tcagtggacg
     3841 ataagctaaa tattcaatta caacacctag gaaagcagac cccagcattg caattaataa
     3901 agctaggaaa aaattaagat gaagatgatt aatcaaataa tagcccatga aagcgcccat
     3961 catatagatg tccccatgag caaaattaat cagcttaata atgccatata ccatagtata
     4021 tcctaaggca agtaaagcat atatggatcc taagataagt ccattaacga gctgttgaag
     4081 catattataa ccatactttc tattatatat tattgtcgaa ttattctgaa aaattacttt
     4141 ttaaaaaagg ggagctgaaa attaaactcc catttttcta gagaatactt attttgctga
     4201 aataatgtta acgctacttg tttttccatc ctctaatttg acaacgtaag ctgatttaac
     4261 aacattgtga tttttatcaa tagacatttt ccctgtcaca cctttaaaat ctttcagctt
     4321 agctaaagct tttttaatat caattgaagt tttagctcct ttagcagatt tagctgccat
     4381 atacacagcg tcgtatgata aagccgagaa catggatggt tcttcaccat atgccttaac
     4441 gtaatgatca tggaaagctt tagctttggt tgatccttgt gtagtgaaac ctgtcaaata
     4501 ataaacgttt gaagctccta caggtgttgc cgattgcaca aatttcggac tatcaaaacc
     4561 atcaggccca agaaccggtt tagagatacc taaatcacgc gcttgcttaa ctattaatcc
     4621 tgtctcggta tagtaacctg gcatcacaat agcatcatat tctttccctt tcaacttagt
     4681 caatgacgct tggaaatcag tatcaccagc ggagaatgtc atactatcaa caatttttcc
     4741 actataactt tccttaaatg attttgctac cccctttgag taatctgatg aattatcata
     4801 aaatagaaca acttttttag ctttaaggtt gtctgtagca tactgagaca atagctttcc
     4861 ttgataatta tcgacaaaag tagctcggaa gaaatactga tttaaggttt ttccatcaga
     4921 accatagact aaattgtctt gtgtcgcagc aggtgcaatc attggaactg ctgctgcatt
     4981 tacttttgga gttgaagatg cagctgcacc cgatgttgct ggtccgataa tgacattagc
     5041 tcctttggta accaagtttg tggcaaccgt tgctgcctct gcattttctg atttattatc
     5101 ctgtgatagg acttttaact tcttgccgcc aacccctcca gcagcattaa tttcttttac
     5161 tgctaagtca gctccattct tcattgaatt tccatacgaa gaaacgtttc ctgataattc
     5221 ccagttatac cctaccttaa tatctttccc tatttttgtt cctgtcgaag atgctcctcc
     5281 tccgacattt ccacatgctg ctaaaagaac tgtactagct aaaacaagcg ctcctaaact
     5341 aagtctcttt tccatatttt tctccttttt tgaaaatggt aactaaaata atacagaatt
     5401 atcagaatat tgtcaatagt ttttaaattg tttttttgaa aattatagaa actaaataaa
     5461 aaagaaggac atctgaactc cttctttcta ataactacta gtaaatatct taacgatata
     5521 agtttccaac aaaatcctga tcaatatcat caattgctga taaccgaaca tccttaacaa
     5581 attttaaatg ttttatctct ttactaacgc taacaatatc ctctttatta acataaatga
     5641 ctagatatct taatctacga gaatgataat gaacatctcc gtatttatat aatttacgag
     5701 cgtcacgatt atagtaaaga taaacgctca ttcctaaacg ctcttgttta ttcaataatt
     5761 ttttcctttc tgcgtcatat ctgtaattac taagattata acataattcc aacaaataaa
     5821 aaaagagttg gtaaactcaa ctcttttaaa tctattattg taaattatta ttttccataa
     5881 tagcatcaat aaagccataa tcaagtgttt cttgagcact catccaacga tcacgctctg
     5941 catcatcatg gactttttca atagattgac cagaattatc agctaagatt ttttctaaag
     6001 tatgacgcgt ttttaaaaga tgctcagcag cgatagccat atcagattgc tgtgtacctc
     6061 cgcctgttcc gcccattggt tggtggatca tatattctgc attcggtaac ataaaacgtt
     6121 ttcctttagc accacttgaa gcaataatgg ttcccatcga agcagccatc cccataacaa
     6181 tcgtctgtac gtccgattta atgaagttca tggtgtccac aatagcaagt ccagccgata
     6241 ctgaaccacc tggtgtattg acataaaggt aaatatcctt tgtattatct tgtgcatcga
     6301 gaaacaataa ctgtgcaatg atactattgg ccatattatc ctcaacttgg cctgtcaaca
     6361 taataatacg atcttttaaa agacgtgagt aaatatcata agaacgttca ccacgacttg
     6421 tttgttcaat aactactgga atcatatctt tctccatttc tttttatcac tgtcactatt
     6481 ataatcattt ggtcaacaaa ggtcaaataa taactttttt aagaaaaggc tttctgagtt
     6541 cttcagaaaa gccttattca tctcaaatct tattttgtac caaataagcg atccccagca
     6601 tctccgagtc caggaacaat gtagccatgt tcgttgagtt tttcatccaa agcagcggta
     6661 taaatatcta catctggatg tgcttcttga agtgctgcca ctccttctgg agctgctact
     6721 aaacaaacga atttgatact agctgcgcca cgttttttta gagaatctac tgctaaaata
     6781 gcagacccac ctgtagcaag cattggatca actacaaaaa tctgacgttg atcaatatct
     6841 tcaggtaact taactaaata ttcgactggt tggaaagtct cttcatcacg atacataccg
     6901 atatgaccaa ctttagcagc tggcacaagg cttaagaaac catcaaccat accaatacct
     6961 gcacgtaaaa ttggaacaat tgctaacttt ttcccagcta attgtttttg tacagttgtc
     7021 gctactggtg tttggatttc aacatcttct aaaggcaaat cacgagacac ttcatagccc
     7081 attaacatcg caatctcatc aactaactca cgaaaatctt tagtagatgt tgtagtgcga
     7141 cgtaagattg acagtttatg ttgaataagt ggatgtgaaa tgacttgaaa ttttcccatt
     7201 ataatgaccg ctttctattt aattttgagc attaaaaaca ttctaatgct tgtttacatt
     7261 caaagtatta taacataaaa accctttcca tgcgactttt tttatagtct agtcattttt
     7321 tctaaagttt gtcactaatc gcaaaactgc ctcctcaata acagacctag gagctgcgac
     7381 attgattcta gcatgttttt tcccttcttt tccaaaagtt aaaccatcat ttaatattaa
     7441 tttggcatca tatcgaagtt tttcttgaat ttctaaatgc gttaaattat aagcagaaaa
     7501 atctaaccaa acaagataag ttccctctgg tttcatcact ttgatatgag ttttttgcgt
     7561 taattgctca taaaggtatt ctattgatcc ttctaactcc atttttaggg cctttaacca
     7621 ttgtttctct tttgtaaagg caacttctgt agctaataac cccaatgaag aaatttcttg
     7681 ttgattgtta gctatttgcc tcttcttaaa atccgagcgt aacttttcat tttcaattat
     7741 tgcaaaacta tttttagtcc ctgcaatatt aaacgtttta gttgcactag ataaaataac
     7801 actcaattct ttaaaactag aatcaacagt attaaagcta tggtgtacat tatcaaataa
     7861 taccaaatct tgatgtattt cgtcagaaac gaggataaca ttatatcgtt tacagatatc
     7921 tcctattttt tgaatttctc cttttgtcca gacacgccca ccgggattgt gtggactaca
     7981 aaaaatataa agctttacat tattttcaat gatatctttt tctaattgtt taaagtcaat
     8041 ctcaaaatac tgattattat taagtaaact atttgaaact aaatgacgat tattgtattt
     8101 tatagttcta gcaaagggcg gatagactgg ggtattaatt agaacagcat ctccctcctt
     8161 tgtgaaggct tgtatagcaa ctgaaatcgc tggtacaacc ccatcaatta ataataatga
     8221 ttttttatca aattgataac catgttcttg cacttcccaa ttagagatag cttggtataa
     8281 actgtcttta ggactatcat agccaaaaac catttggtgt gaaaaatctt caatagcctc
     8341 cgacatttct ggaaagatag gaaaatccat atccgcaatc caaaggggaa ggatttcttg
     8401 atctttttga accgccttcc attttatggt attacttgaa aaacgttctg gtaaacttgt
     8461 gaaatcatat gtaatcataa ttactcctga ctattctaca gctaaaaata tcccttgtcg
     8521 ccttggtgac tattttacca tttgtttttg atactatata ctaattaaca ttctaaagat
     8581 aaaagtgacc attttaaacg atcactttct ttattatttt aatttatcat tttcataagt
     8641 atggcttaat tctaaattag gaaagctttg ctgcctaagg gcttcataaa caatcataca
     8701 aacagtgttt gaaacattca agctacgcac atgctcatcg ttcatgggaa ttcgtaaggc
     8761 cttttcggca tgctcgcgca taaaagtttc tggcaatcct ttatcttcac gaccaaacat
     8821 aaaataatgg tcttgatcat cattgtaatt ttcatctgag taaaccttgt cagcaaattt
     8881 gctaattagg tgaactttcc ctcgacatga caacataaat tcttccaaac catcatagaa
     8941 actaacatct agcttatccc agtaatccag tcccgctcgt ttcatttttt tatcatcaat
     9001 aggaaatccc atcggtcgta tgatgtgcaa aggagcattt gttgccgcac aagttctagc
     9061 aatattacca gtatttgctg gaatctgtgg ttcaaataaa acaatatgat ttcgtccgct
     9121 atcggaacga tgatttttct gcgttagtgt ttcaatattc atctcatcat cactttctag
     9181 aactcattct gactcgcaaa aaaaccacac tgctcggagt caatctcagc aaacaatgtg
     9241 gtttatggtg gtttgttaac ttaaatcata acaggtttga tttaaactaa caaatcgttg
     9301 acactattat agcacttcag tctacaaagt caacctattc atgagttttt gggttatttt
     9361 tttgatagat ataatgacaa atcaaacaga ataataagca aaaacattgg aaaattaaaa
     9421 ccgtaaagaa attacttaac aaactcccga taaggaggat caaaccgcct aaaattgcta
     9481 gaatcggtga aactaagccg gtaaatttat ttttaatatc ttttttgaga taaaggctga
     9541 gtactaaaac ataaagaatg attaaactgg tattattaaa caccacagca atttcactaa
     9601 tatcactacc cggcaataag ttaaattttg taaccataaa atgaacaaaa agccaaaaaa
     9661 tagctacagc cgtcaccgtc aaactagctg gcaatgacat ttgatacttc aaatttatgt
     9721 tagccatcct ctcacttttg atccatccgc gttccgcaaa agcttgaggt agacgcatcg
     9781 tcccaagtaa aagtccattg gcaacaccta atacagatag aataacaatg aaagataata
     9841 aacgcccaac actaggaccg aaaataatat tagctgcata attaatagca tcattaccgg
     9901 ttgtcataat aaagctagca cctaaaattt gtgtcaaacc atagaaaaat gccaaatatg
     9961 ataataagat aagcgctgga ccaatgacaa aggcaagtgg taaattcttc ttgggattct
    10021 tgacttctgg tgcaatatta acaaaaattg tccaaccatc atatgcaaaa tacaatggta
    10081 ccagacctga cacccaactc catccaacat ttgacggttg aatagctgtt aaatgttgtg
    10141 gaagttgtgg agctttatca gaccaaaaag caccaattaa agcaattaga actaaaggaa
    10201 tcatttttac aaaggtcgtt aaagattgga atccccctgc aatacgctta gcaaagatat
    10261 tcatgagact aagcgctaaa atataaacag cagctaaaat aatctgtgat tccaaacttg
    10321 atgattcacc taaagtataa aatgcagcta cccatgaaac gattgctgtt aaagttggaa
    10381 gatacaaaaa agacgcaaac aagcctaaag ttgctgctaa agctgggcta acatattttt
    10441 cgtagtaaga aaaaatacca ccactttcac tcgttctaat agctagctca gaaattgaca
    10501 aacttccaaa aacaatcgaa aaagaaccta gtactaaaat taccatgcct aaaaagacat
    10561 ctccaccagt aaatttaagg atgtcatcaa ctttaaaata aatgcctgaa ccaatcacta
    10621 cacctacaat catagcaatt gtagtcatta atccataggt ctgtttaata gaattatgat
    10681 tctccataat atctccattt ctactgtacg cttaaaggtt aattataacc taatttaggg
    10741 cttgtcacaa gtttatctgt gttatttttc accagattct gataagagaa tcatattact
    10801 cttatcagaa agatgaaaac gttttttaac cataattctc gtgcattttt actaaaaaaa
    10861 aggtatcata gagtttatta ttggaggtaa aaatgagaat aattgttgtc ggcggaggta
    10921 aagtcggaac cgcactttgt cgctcacttg ttgctgaaaa gcacgatgtt gttttaattg
    10981 agaaaaaaga aaacgtcctt aaacgtgtca ctaaacagca tgatattatg ggaattgttg
    11041 ggaatggagc aaactataaa attttagaac aagccgaagt caaaaattgt gatattttta
    11101 tcgctatcac tgatcgagat gaagttaata tgatttcagc agttcttgcc aaaaaaatgg
    11161 gggcaaaaga gactgttgtt cgcatgcgta atcctgagta ttctaatcct tacttcaaag
    11221 ataaaaactt tcttggcttc tcttcagttg tcaaccctga attgttagca gcacaatata
    11281 tcgctaatac catagaattt ccaaatgcaa cttcagttga acacttcgcc aacgggcgtg
    11341 tgatgttaat ggaatttaaa attttagaag gaaataaact ctgtcatact tcaatgagtc
    11401 aaattcgtaa aaaatttggt aatattgtta tttgcgcaat tgaacgcgat ggcaaattga
    11461 ttattcctga tggcgatgca actatccaag tcaaagataa aatctttgta actggaaatc
    11521 gtattgaaat gatacttttc cataattacg ttaaaaataa agttgtcaaa aacttgatgg
    11581 ttatcggtgc aggacgcatc gcctactacc tactcaatat ccttaaaaat accaatacac
    11641 acgtcaaact agttgaactc aaccaagagc aagctgaata ttttagtcaa gaatttccca
    11701 atgttcctgt cgttcatggg gatggtactg ctaaaaatat tttgttagaa gaaagcgtta
    11761 caagttttga cgcagttgct actttgactg gtgttgatga agaaaatatc attacctcaa
    11821 tgttcttaga aagtatcggt atccctaaaa acattaccaa agttaatcga actagcctac
    11881 tagaaatcat tgacgataaa caactatcaa gcattatcac acccaagcgt attgccgtag
    11941 accatgtgat gcactttgta cgcgggcgtg taaatgctca ggattccaac ctagaagcta
    12001 tgcatcatat tgctaacgat cgcattgaaa ccttacaatt tgaaataaaa gaaactagca
    12061 aattagcaaa tcgtagcctt gcatctttaa aattaaagca aaatatttta attgcagcga
    12121 ttattcgcaa taataaaacg attttcccaa ctggtgagga tgttttaaca gtgggtgatc
    12181 gtatcgttgt tatcacctta ttaaaaaata ttactagaac tagtgatatg ttagcgaggt
    12241 aggaatatga ataaaagtat gattcgtttc cttctctcaa aacttttgct aattgaagct
    12301 gctcttctag cgattcctct aactgttgga cttatttacc gtgaaccgca aagtgtcatg
    12361 atgagtatag tcattaccat gattatttta atcatccttg gattactggg ttctctcttt
    12421 aaacctaaaa attatcatat ctacactaaa gaaggcatgt taattgttgc actctgctgg
    12481 atactttggt ccttctttgg cgcccttcca tttgttattt cagggcaaat ccctaatatt
    12541 atcgatgctt tctttgaagt atcttctggt tttacgacaa caggagcaac tattcttgat
    12601 gacgtcagtg tcttatcacc cgcgctctta ttttggcgta gctttaccca tcttatcggt
    12661 ggaatggggg ttttagtatt tgcccttgcc atcatggaaa attctaaaaa tagccattta
    12721 gaggttatga gagctgaagt tcctggacct gtatttggta aagttgtctc gaaacttaaa
    12781 aaaactgcac aaattttgta tttgttatat cttcttatgt ttgcagtatt tgcagtaata
    12841 ttatattttg caggtatgcc tttctttgat agtattatca tagcgatggg aactgcaggt
    12901 acaggtggtt ttgcagttta caatgatagc attgctcatt ataatagtcc tctgataact
    12961 aacttagttt ctattggtat gcttatcttc ggggttaact ttaatcttta ctatcttctt
    13021 ttactccgaa aaattaaagc attctttggt gatgaggaac taaaaaccta tctccgcatc
    13081 gtggcaatag caactttcat gattgctcta aacgtcatcg gtatgtatga taattttcga
    13141 caaggcctcg aacatatttt ctttgaagta tcagctatta ttacgacaac tggttttggc
    13201 gtgactgata tcactcgttg gcctttgttt tcacaggtta ttttgctatt cttgatgttt
    13261 atcggtggtt ctgctggctc tacagctggg ggctttaaag tcatgcgtag cttaatccta
    13321 gcaaagattg cacgtaacca agtattgtct acactctatc ctaatagagt catgtcacta
    13381 cacattaata aaagcgttct agataaaaat acacaacatg gtgttttaaa atatctaact
    13441 atctatttag ctatcttcat ggcactcgtt cttgttttaa cgcttgatac taatgatttt
    13501 ttggttgtta tcagtgctgc agcctcatgt tttaacaaca taggcccact cttaggtagc
    13561 aatgaaactt tctcattctt tagtccattc tcaaaactcc ttctttcatt tgctatgata
    13621 gcaggacgtt tagaaatata ccctgtttta ttgatgttta ttcctaaaac atggtcaaaa
    13681 ctataattac aaataataaa acggatatat caatctgata tatccgtttt attacgtctt
    13741 aagctaaaat gatcagggac aggatcattt cctccgtggg ctaagggatg acatcgcaaa
    13801 atacgtgcaa tccccatcaa cacacctttt agaccatgtt tttgaatagc ttctatcata
    13861 tacgtagagc aagttggacg ataacgacag ctagctggga aagctggaga aatatttttt
    13921 tggtaaaagc gaactaagaa aatcaaaaaa gatttaagca ttaagattta ttaatagccg
    13981 cattatgcaa ctgactaatt tcttttttat ttaatcgtcg tgcttcccca ggacggagac
    14041 ccgttaaatc taaggtaccg aattgtgtac gagatagctt gtccactaat aacccaactt
    14101 gttcaaacat tttcttaact tgatgattac gaccttcatg aatagttaat tcaactactg
    14161 aacgattttt ttcatgatcc acttttatga tagtatagcg agcaggtttt gttttcttac
    14221 catcgataac aacgccacgc gttaatggtc gaaggttttc tttagtagct attcctttaa
    14281 cacgtgctaa ataaacttta tcaatttcat tacgaggatg aatcatttta tcagtaaaat
    14341 caccatcatt agtcaaaatt aaaagtccag ttgtatccca atccaagcga ccaacaggat
    14401 atatccgttc tttgacttgt ggcaacaagt caataactgt cttacgtcct ttatcatctg
    14461 agacacttga aataacacca cgtggtttat ttaagagata atataccttc tcttcgttgt
    14521 aaattggact tccttcaatt tcaaccagat caccagcctt tacttgggtc gccaattcat
    14581 taaccacttg accattaatc gtgaccattc cttgcttgat taattcctca gcttttctac
    14641 gacttgcaat ccctgcatgc gcaatatatt tattaattct cattgttttc cttatcttct
    14701 gtattaattg aatccattga aaataacgat acttcttcgt caactaattc aattgaagag
    14761 gcgtcaatta agtcatctaa ctgattaatt cccatataat ctaagaaata attagttgtc
    14821 tcataaaggt taggacgtcc caacacttct ttctttccag cctctttaat taaaccaaaa
    14881 gctatcagtc tcgttatagc tccactggaa ttcacgccac gaatatcatc aatttcaatc
    14941 ctagtaatag gttgtttata agcaataata gatagaactt ccaaacttgc ccgagataga
    15001 ctctgattaa tcggagcctt cgcataatct ctcaacaaag tcatataact atcctttgtt
    15061 accaatttat aagtttgtgc tgtctcaagc aataatagac tagaatcgtc atcttcttca
    15121 tatcgtttag ccaacttttc tagttgctgt attaatgctg atggtgtcaa agacaataat
    15181 tctgccattt gacgcaaact cagtccatct tcacctgcaa caaaaagaag agcttctatt
    15241 gagcctaaat aagtcatttc tcttcctttc ttaagataac ttgactaaaa ttactgtctt
    15301 gttctactgt gatttgatgt aattttatca actctaacat tgccaaaaat aaagttatca
    15361 tttcagattt tgtttggcaa tccgcaaaca cttcttgtaa tgtcgttttc tttttaagat
    15421 taaaatgtct ttcaataacg atcatcatat cttcaatacg gtagtcttct ttctcaatta
    15481 ccgtttgact atttgacaat tctttttgtt tttgggacat catttgagaa aaagtcaaaa
    15541 aaagatccat caccgattta tcgtgtagaa gaattgcatc ttcaaagata acttcttgtt
    15601 ttggttttga aaaatatttg gcgcgttcct gatgctgatt agctaactct tcacttagca
    15661 ctttaaaacg tctatattcc tctaattggc tcaacaattc catttctgga tcatcttcaa
    15721 taggatttga ctccactact ttaggtaata agttacggct ttttatcaac atcagctgac
    15781 ttgccataag catatactca ccagctactt ctagacgcat cgcctgtaat gttgctatat
    15841 aagcaaggta ctgctcaata acttccacaa tcggtacatc gtatatatca acctcatatt
    15901 tagatacgag gtgcaagagt aagtctaggg gcccctcgaa atcttttaac ttaatatcca
    15961 ttatctataa tatctctcta aagtgatagg agtttttaag cctagaaggc gactgagctc
    16021 cgtcatcgtt ttgccatttt ctttttgctt tagaataaat tgttctcgca gaagttgtgc
    16081 agttagttgt tgctcattct tctcatttaa gaaatctgtt aattgattaa aaaaccattg
    16141 tctagaataa ggcttaccgt tatgctcaaa gactaagtca ccatcttcct ctaaagattc
    16201 caaaagaaaa ggaagcaagt cttctggaag ttttaaaata cgcttcatct gcgatttttc
    16261 aatacttaag atattaaaag ttgtatcaaa atcagccttc ttcaaattag caatttcagc
    16321 cggagttaaa cctaaagata aaatcaacaa agcaatcaaa cgaccactcg gataatcagt
    16381 atcttgatag aaatttgata aatccattat ttgagacttt gtctgagcca aagtgatttt
    16441 ttcagtctct tgtagtctgt aaaattcctt cagcgttcct ctattatata aaaagaaaag
    16501 aaattgattg actgcagata actttcgctt acgagctgtt aacttaaatt ca
//
LOCUS       DATRMA010000040        16411 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.040, whole genome shotgun sequence.
ACCESSION   DATRMA010000040 DATRMA010000000
VERSION     DATRMA010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16411
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.040"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            complement(39..512)
                     /locus_tag="VBC28_001245"
     CDS             complement(39..512)
                     /locus_tag="VBC28_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEO6815230.1"
                     /translation="MIAKEFESFLLNHLDHYLIPAEDVAIFVDTHNADHVMLLLASNG
                     FSRVPVITKEKKYVGTISISDIMGYQSKGQLTDWEMAQTDIVEMVNTKIEPINEAATL
                     TAIMHKIVDYPFLPVISDQNDFRGIITRKSILKAINSLLHDFTDEYTITPKNNDK"
     gene            complement(509..1030)
                     /locus_tag="VBC28_001246"
     CDS             complement(509..1030)
                     /locus_tag="VBC28_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEO6815231.1"
                     /translation="MAIRKLVVMSDSHGDRDIVKDIKNHYLGKVDAIFHNGDSELPSS
                     DPIWEGIHVVTGNCDYDSGYPEVLVTKIDNAVIVQTHGHLHQINFTWDKLDLLAQQED
                     ADICLYGHLHRADAWKNGKTIFINPGSVLQPRGPINEKLYAVVTITDSKVLVEYYTRQ
                     HQPYPNLTKELSR"
     gene            complement(1012..1986)
                     /locus_tag="VBC28_001247"
     CDS             complement(1012..1986)
                     /locus_tag="VBC28_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphate diphosphatase"
                     /protein_id="HEO6815232.1"
                     /translation="MTKTIFESKTEGNWFLGSFQAFNYFTCFGNDESYEAIQDVFHRL
                     LSTLKVEGLQLHVVQMTSDFQLLAFLVDMINQEYSRHIKVTQHKGAILVSEDDQLFLV
                     HLPKEGTSLEKFFDLKNDNNFGDTILIATHNEGKTKEFRELFGKLGLKVENLNDYPDL
                     PEVEETGMTFEENARLKAETISKLTGKMVISDDSGLKVDALGGLPGVWSARFSGPDAT
                     DARNNAKLLHELAMVFDKERRSAQFHTTLVVSAPNKESLVVEAEWPGYIGTEPKGENG
                     FGYDPLFIVGEGSRTAAELSAQEKNNLSHRGQAVRKLMEVFPKWQLEN"
     gene            complement(1983..2777)
                     /gene="racE"
                     /locus_tag="VBC28_001248"
     CDS             complement(1983..2777)
                     /gene="racE"
                     /locus_tag="VBC28_001248"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000378350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEO6815233.1"
                     /translation="MDSRPIGFLDSGVGGLTVVKEMFRQLPEEEVIFIGDQARAPYGP
                     RPAQQIREFTWQMVNFLLTKNVKMIVIACNTATAVAWQEIKEKLDIPVLGVILPGASA
                     AIKSTNLGKVGIIGTPMTVKSDAYRQKIQALSPNTAVVSLACPKFVPIVESNQMSSSL
                     AKKVVYETLSPLVGKLDTLILGCTHYPLLRPIIQNVMGAEVKLIDSGAETVRDISVLL
                     NYFEINHNWQNKHGGHHFYTTASPKGFKEIAEQWLSQEINVERIVL"
     gene            complement(2952..3191)
                     /locus_tag="VBC28_001249"
     CDS             complement(2952..3191)
                     /locus_tag="VBC28_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HEO6815234.1"
                     /translation="MSTTIWILLIIVALFGGLVGGIFIARKQIEKEIGEHPRLTPDAI
                     REMMSQMGQKPSEAKVQQTYRNIVKHAKTAIKTKK"
     gene            complement(3349..3891)
                     /locus_tag="VBC28_001250"
     CDS             complement(3349..3891)
                     /locus_tag="VBC28_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6815235.1"
                     /translation="MSKKTTQMVSYTSILVAFAIMIPIIMPAKIIIGPASFTLASHVP
                     LFLSIFISVPVAILVALGTGLGFLLAGFPIVIVLRALSHIGFALIAAFLIKSKPSLLM
                     SKWQTLLFAVAINIIHGLLEFITVYIITMTSNSSSTYLWSLFSLIGLGSLLHGLVDFY
                     IALFIWKWMTQKLGKTLRIR"
     gene            complement(3953..4474)
                     /locus_tag="VBC28_001251"
     CDS             complement(3953..4474)
                     /locus_tag="VBC28_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HEO6815236.1"
                     /translation="MKAQERRENILTTLKGTKEAISASTLAKIFSVSRQVIVGDIALL
                     RAQQCDIISTPKGYLMSSALSTHQFTARLVCQHGIEQTEEELEIILRYQGIIMNVEVE
                     HPIYGMLTAPLNIQSQKDIDNFTAKLKVSNAELLSSLTDGLHTHMISCQDQSVFDQIC
                     EALKKAGILYIDK"
     gene            complement(4542..5231)
                     /locus_tag="VBC28_001252"
     CDS             complement(4542..5231)
                     /locus_tag="VBC28_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEO6815237.1"
                     /translation="MNDNVIYTQSDSGLNQFFAKIYGLVGIGVGLSAAVSAIMLYMFP
                     QNMIAIMQKMPGLYFGAIILELVLVFVASGAARRNTPAALPLFLIYSALNGFTLSFII
                     ARYTQTTVLQAFITSAAVFFAMALIGAKTKKDLSGMRKALMAALIGILIASLVNLFIG
                     SGGMSYIISIVCVIIFSGLIAYDNQMIKYVYNSQGGQVADGWAVSMALSLYLDFINLF
                     LNILRLFARND"
     gene            complement(5254..5757)
                     /locus_tag="VBC28_001253"
     CDS             complement(5254..5757)
                     /locus_tag="VBC28_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003087049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEO6815238.1"
                     /translation="MKKYQDDKDFMDLVGHLIDHPRFQKLEAIVQHHHSTRLEHSINV
                     SYTSYKIAKKFGWDASSTARGGLLHDFFYYDWRVTKFNKSHAWVHPRIAVRNARKLTD
                     LNAREEDIILKHMWGATIAPPRYKESYIVTMVDKYWAVREASRPLKRIFKKPIRFSRK
                     FLGSHNH"
     gene            complement(5795..6538)
                     /locus_tag="VBC28_001254"
     CDS             complement(5795..6538)
                     /locus_tag="VBC28_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000207503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEO6815239.1"
                     /translation="MTTIITSKSNNLIKKTKKLLQKKYRKSSYLIEGWHLFEEAEKYG
                     AQFLNIFVTETAIDRLRKPERAIVVTDDVLKELTDSQTPQGIVAEIAFQETRWTDIKK
                     GRFLVLEDVQDPGNLGTMVRTADAANFDAVFLSQKSADLYNQKTLRSMQGSHFHLPVF
                     RVEIEQFVNFCKAEGITMIATTLSEQSVNYKNLPKYDYFALIMGNEGQGISKTMTEEA
                     DVLAHIEMPGQAESLNVAVAAGVVIFSLI"
     gene            6570..6848
                     /locus_tag="VBC28_001255"
     CDS             6570..6848
                     /locus_tag="VBC28_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEO6815240.1"
                     /translation="MKKVHLIVSGRVQGVGFRYATYSLALEIGDIYGRVWNNDDGTVE
                     ILAQSTDSNKMTQFIQKIRKGPSKWSKVTYVDIKLDNFDDFNDFKMSN"
     gene            6933..7865
                     /gene="yidC"
                     /locus_tag="VBC28_001256"
     CDS             6933..7865
                     /gene="yidC"
                     /locus_tag="VBC28_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEO6815241.1"
                     /translation="MKKTLKRILFSSLSLSMLLLLTGCVSVDKAGKPYGVIWNTLGVP
                     MANLITYFAQHQGLGFGVAIIIVTVIVRVVILPLGLYQSWKASYQAEKMAYFKPLFEP
                     INERLRNAKTQEEKLAAQTELMTAQRENGLSMFGGIGCLPLLIQMPFFSAIFFAARYT
                     PGVSSATFLGLNLGQKSLTLTVIIAILYFVQSWLSMQGVPDEQRQQMKTMMYLMPIMM
                     VFMSISLPASVALYWFIGGIFSIIQQLVTTYVLKPKLRRKVEEEYTKNPPKAYKANNA
                     RKDVTNSTKATESNQAIITSKKTNRNAGKQKRRG"
     gene            complement(8065..8730)
                     /locus_tag="VBC28_001257"
     CDS             complement(8065..8730)
                     /locus_tag="VBC28_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000611496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEO6815242.1"
                     /translation="MINWDAIFNLELAVKAFPSVIQGLPYTIGLSLVGFILGAIVGFF
                     VALMKMSHFRLLRYLANIHISLMRGIPLMVLLFLIYFGLPFIGIQLDAVTASIVGFTM
                     MSSAYISEIIRAALLAVDHGQWEAARALGLKTPTIYRGIIIPQATRIALPSLSNVLLD
                     MVKSSSLTAMITVPDIFNNAKIVGGTYSDYMTAYILVALIYWVICTLYAIIQDWWEKR
                     LLY"
     gene            complement(8754..9611)
                     /locus_tag="VBC28_001258"
     CDS             complement(8754..9611)
                     /locus_tag="VBC28_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEO6815243.1"
                     /translation="MKKTILLGLVGLSAMTLAACSNGQSSKETTWDNIKKDGVLKVAT
                     PATLYPTSYYDDHKKLTGYEIDMMKAIAKKLKIKVKFVEVGVAESFTSVDSGKVDVAV
                     NNFDTTPERLKKYNFSQPYKYSVGGMIVRADGSSKITAKDLSDWKGKKAGGGAGTQYM
                     KIAKQQGAEPVIYDNVTNDVYLRDVSTGRTDFIPNDYYTQVIAVKYVTKQYPDIKVKM
                     GDVKYNPTEQGIVMSKKDKSLKTKIDAAIKDMKKDGSLKKISEKYYAGQDLTKEPAGT
                     DKIPVIKVK"
     gene            complement(9772..11232)
                     /locus_tag="VBC28_001259"
     CDS             complement(9772..11232)
                     /locus_tag="VBC28_001259"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEO6815244.1"
                     /translation="MVFKDATAMVQAIKQHKISSQELVEQAIYKIEEQNVSVNAVVSK
                     QYNEARQAAKYANESNAPFAGVPILLKDLGQNQKGQLSTSGSQLFKHYHAKQTDYLVQ
                     SFEKLGFIILGRTNTPEFGFKNISDGQLHGNVNLPFDHSRNAGGSSGGAAAAVSSGMV
                     PIAGASDGGGSIRIPASFNGLIGLKPSRGRIPVGPSSYRGWQGASSHFALTKSVRDTK
                     RLLYYLQSYQVESPFPLKKLSKESLFEFSVSKPLKIAVLMDSPLKTKVSSEAKAAIKE
                     AADFLSQKGNHLELVEQPLDGIHSMKTYCMMNSVETAAMFDDIEKSLGRSMEFSDMEL
                     MTWAMYQSGQRVLAKDYSKLLDSWDQFAATMARFHENYDLILTAATNQPAPFHGQFDL
                     DETLQKQLRHMGEFSVSEQQDLIWKMFEDSMAWTPFTHQPNLTGQPSLAIPTHLTKEG
                     LPLGVQLTAAKGREDLLLAVAELFEKEKQFKGPVYH"
     gene            complement(11335..11817)
                     /gene="greA"
                     /locus_tag="VBC28_001260"
     CDS             complement(11335..11817)
                     /gene="greA"
                     /locus_tag="VBC28_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEO6815245.1"
                     /translation="MAEKTYPMTQVEKDQLEKELEELKLVRRPEVVERIKIARSYGDL
                     SENSEYDAAKDEQAFVEGQIQILETKIRYAEIIDSDAVAKDEVAIGKTVLVQEVGTND
                     KDTYHIVGAAGADIFSGKISNESPIAHALIGKKTGDLATIESPAGSYQVEIISVEKTN
                     "
     gene            complement(11890..13692)
                     /gene="mltG"
                     /locus_tag="VBC28_001261"
     CDS             complement(11890..13692)
                     /gene="mltG"
                     /locus_tag="VBC28_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEO6815246.1"
                     /translation="MTEFNDDQHSNHDQKSFKEQILAELEEANRLRKLREEELYQKEQ
                     EAKEAARRTAQLMADYEAQRLKDEQEARAKALETKQRLEEQEKARIEAKLLAEAAREE
                     ERRQAEQALASQEEQVINQGMEPSRELDSGSKSSEFRTTENVPDIDLKADKTDVATAV
                     PNQETEEIFLVRATDIPTEGENVKLGEISELEPVAKEPIRVEDLSKEEEGIALSAKNK
                     HNKRERRQKADNVAKRIARILISIIILVLLLTAFVGYRFVDSAIKPVDSNSNKFVQVE
                     IPIGSGNKLIGQILEKAGVIKSATVFNYYSKFKNYSNFQSGYYNLKKSMTLDQIAAEL
                     EKGGTAEPTKPALGKILITEGYTIKQIAKAIESNKIDTKTTSTPYKADDFLKLVQDET
                     FIKKMVAKYPNLLGSLPDKSKAIYQLEGYLFPATYNYYKDTTLEGLVEDMISTMNTKM
                     APYYNTIKAKNMSVNDVLTLSSLVEKEGSTDEDRRKIASVFYNRLSAGQALQSNIAIL
                     YAMGKLGDKTSLAEDAQINTSIKSPYNIYTNTGLMPGPVDSPSISAIEATIKPASTDY
                     LYFVADVKTGNVYYAKDFETHKANVEKYINSQIN"
     gene            complement(13779..14282)
                     /locus_tag="VBC28_001262"
     CDS             complement(13779..14282)
                     /locus_tag="VBC28_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000586872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO6815247.1"
                     /translation="MIIRNGCLEDLQQVISIEQINFSEAEAASKKAMQERLTIMTDTF
                     LVAEINGRLAGYIEGPVIKGRYLTDDLFHKVSEFPVRVGGFIGITSLSIHPDFKGQGI
                     GTALLAAMKDLVVSQERDGISLTCHDDLISFYEMNGFKDEGESDSKHGGSLWYNMIWN
                     NPTRSIC"
     gene            complement(14368..15699)
                     /locus_tag="VBC28_001263"
     CDS             complement(14368..15699)
                     /locus_tag="VBC28_001263"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEO6815248.1"
                     /translation="MSKTYHFIGIKGSGMSALALMLHQMGHNVQGSDVDKYYFTQRGL
                     EQAGVTILPFSPNNISEDLEIIAGNAFRPDNNEELAYVIEKGYHFKRYHEFLGDFMRQ
                     FTSLGVAGAHGKTSTTGLLAHVLKNITDTSFLIGDGTGRGSANANYFVFEADEYERHF
                     MPYHPEYSIITNIDFDHPDYFTGLEDVFNAFNDYAKQVQKGLFIYGEDPKLHEITSEA
                     PIYYYGFEDSNDFIAKDITRTVNGSDFKVFYNQEEIGQFHVPAYGKHNILNATAVIAN
                     LYIMGIDMALVAEHLKTFSGVKRRFTEKIIDDTVIIDDFAHHPTEIIATLDAARQKYP
                     SKEIVAIFQPHTFTRTIALLDEFAHALSQADSVYLAQIYGSAREVDNGEVKVEDLAAK
                     IVKHSDLVTVENVSPLLNHDNAVYVFMGAGDIQLYERSFEELLANLTKNTQ"
     gene            complement(15709..16326)
                     /locus_tag="VBC28_001264"
     CDS             complement(15709..16326)
                     /locus_tag="VBC28_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6815249.1"
                     /translation="MNRGSRRFPLIADDEPVMSPLVKMNLYDNEDLINNIRDFYQEKT
                     YQSMVKSNYEHEEISHPKVIENDPVPPQSFVKKATELSKSRQEAKRSVREKRQAYYAK
                     QEFKAPSKEAFQQQLKATVPKKQTQRKVTELSHLSDRLQQESYILAEIPIIFQEPDNT
                     PNPKTKKNNFDFLKRSQVYNKQDNQFHKERAKAQELNLTRFKDIN"
BASE COUNT         5110 a         3236 c         2575 g         5490 t
ORIGIN      
        1 tgagagataa tttcttactc tcaataaaat tattaatatc atttatcatt gtttttagga
       61 gtgatagtat attcgtctgt aaaatcatgt aggaggctat taatagcttt taaaatcgat
      121 ttacgggtta taatgcctct aaaatcattt tgatcactga taacaggtaa aaatggataa
      181 tctacaatct tatgcataat tgctgtcaga gtagctgcct cattaatcgg ctcgattttt
      241 gtattaacca tctcaacaat atcagtctgt gccatctccc aatctgttaa ttgaccttta
      301 gattggtagc ccataatgtc tgagatacta atagtaccta catatttttt ctctttagtg
      361 ataactggta cacgtgaaaa accattactt gctaatagta acataacgtg gtctgcattg
      421 tgtgtatcca caaaaatagc aacgtcttca gctggaatta gatagtggtc caaatgattt
      481 agcaaaaacg actcaaattc tttagcaatc atcttgataa ttcctttgtc aagttaggat
      541 agggttggtg ttggcgagta taatactcta ccaaaacctt actatctgtt atagttacaa
      601 cagcgtaaag cttctcattt attggtcctc ttggctgcaa aacactacct ggattgataa
      661 aaatagtttt accatttttc caagcatctg cacgatgaag atgaccatat aaacaaatat
      721 ccgcgtcttc ttgctgagct aataaatcca atttatccca ggtaaaatta atctgatgga
      781 gatgaccatg ggtttgaaca ataacagcat tatctatttt tgtaaccaaa acttccggat
      841 aacctgaatc ataatcacaa tttcctgtga ccacatgaat gccttcccat ataggatctg
      901 agctaggaag ttcggaatcc ccattgtgaa aaatagcatc cacttttccc aaataatgat
      961 tctttatatc ttttacaatg tcacgatctc cgtgagaatc actcattaca actaattttc
     1021 taattgccat tttggaaata cctccatcaa tttacggaca gcttgcccac gatgtgatag
     1081 attatttttt tcttgtgcgc tcaattcagc tgctgtccta ctaccttctc cgacaataaa
     1141 gagcggatca taaccaaagc cattttcccc cttaggttcc gtaccaatat aacctggcca
     1201 ctctgcttca actaccaaac tttctttatt tggagctgac acgactaagg ttgtatgaaa
     1261 ctgtgctgaa cgtctttctt tgtcaaatac catggctaat tcatgcaata atttggcatt
     1321 attacgagca tctgtcgcgt caggacctga aaaacgagca gaccataccc ctggcaaacc
     1381 acctaaagca tctactttta atccggaatc atctgaaatt accatttttc cagttaattt
     1441 tgaaatagtc tctgctttaa gtcgagcatt ttcttcaaag gtcataccag tctcttcaac
     1501 ttctggtaaa tctgggtaat catttaaatt ttcaactttg agtcctaatt tcccaaataa
     1561 ttctctaaat tccttcgttt ttccttcatt gtgagtcgca attaatatgg tatccccaaa
     1621 attattatca ttctttaaat caaagaattt ttctaaagaa gtgccctctt taggtaagtg
     1681 aactaagaac aactgatcat cttcactaac taagatggcc cctttatgtt gagtcacttt
     1741 gatatgcctt gaatactctt gattaatcat atcaacgagg aatgctaata attgaaaatc
     1801 cgaagtcatc tgcacaacgt gaagctgtag accttcaact ttcaaagttg ataatagacg
     1861 atgaaaaaca tcttggatag cttcgtaaga ttcatcatta ccaaaacaag taaaataatt
     1921 aaaagcttgg aaactaccta aaaaccaatt gccttcagtt ttactttcaa aaattgtttt
     1981 tgtcataata cgatacgctc cacatttatt tcttgactaa gccattgttc tgcaatttct
     2041 ttaaaacctt ttgggctggc ggttgtgtaa aagtgatgac caccgtgttt attttgccaa
     2101 ttatggttta tctcaaaata gttcaataaa acagaaatat cacgaacggt ttctgcgcca
     2161 ctatcaatta atttaacctc agcccccata acattttgaa tgatgggacg taatagggga
     2221 taatgcgtgc aacctaaaat taaagtatct aatttaccaa ctaatgggga caacgtttca
     2281 taaaccacct ttttggctaa actagaagac atctgatttg attccacaat tggaacaaat
     2341 ttcggacaag caagggatac cacagcagta tttggagaca aagcttgaat tttttgacga
     2401 taagcatctg atttaacagt catgggagta cctataatac caactttccc taaattagtt
     2461 gatttgatag ctgcgctagc tcctggtaaa ataacgccta aaacagggat gtctagtttt
     2521 tctttaattt cttgccaggc aactgcagtt gctgtattac aagctataac aatcatctta
     2581 acatttttag tcaataagaa gttaaccatc tgccaggtaa actctctaat ctgttgagca
     2641 ggtctaggac catacggagc tctagcctga tctccaatga agattacttc ctcttctgga
     2701 agttgacgga acatttcctt aacaaccgtt aaaccaccta caccagaatc tagaaagcca
     2761 ataggcctac tatccatttt tattcctcca atattagtga ctaaaagagg ctggacaaca
     2821 gtcctagcct ctctatacaa taaccaatca gtcaaatcta cacttgacca agtttgccca
     2881 aatcttgtct agaaatctga tggactatca ctcactattt catcactaaa cgcttcaatg
     2941 ctaaaacact cttatttttt agttttgata gctgtttttg cgtgttttac aatattacgg
     3001 tatgtttgtt gaactttagc ttcactaggt ttttgtccca tttgactcat catttcacga
     3061 atagcatccg gtgttaaacg agggtgctct ccaatctctt tttcaatttg tttacgagca
     3121 ataaaaatgc ctcccacgag accaccaaac aaagcgacga taattaataa aatccaaata
     3181 gttgttgaca tctattcatt ctcctcattt atttacatga ctcattatag caaaaaaaga
     3241 aagagtttac aaatatatgt aaacaaaatc aaagcctcta tcgtccttgt cacgtcaata
     3301 cactattaaa ttttcactag agcatggtta agcacatagt cgagtccttt atcttatcct
     3361 aagtgttttc cccaactttt gtgtcatcca tttccaaatg aagagagcta tgtaaaaatc
     3421 taccaatcca tgtagcaaag agcctagtcc tatcaaagaa aatagtgacc ataaataagt
     3481 tgaactactg ttagaagtca ttgtgattat ataaacagta ataaattcga gcagaccatg
     3541 aattatgtta atagctacag caaataacaa tgtttgccat ttactcatca ataaagaggg
     3601 ctttgactta atcaagaaag ccgcaattaa tgcaaatccg atatgtgata aagctctaag
     3661 aacgattaca atcggaaaac cagctagtag aaatccaagt cctgtcccta aagctaccaa
     3721 tattgcaact ggaacagata taaaaatact taaaaataaa ggtacatggc ttgctaaggt
     3781 gaaagaagca ggcccaataa ttattttagc aggcatgatt ataggaatca tgattgcaaa
     3841 agctactaaa atagaagtat aagaaaccat ttgtgttgtt ttttttgaca tttttatctc
     3901 ctttgaagct attatacaaa ataaccttac aggtgtaaag acacctgtaa ggttatttgt
     3961 ctatgtataa aattcctgct ttttttagag cctcacatat ttggtcaaaa actgactgat
     4021 cttgacaaga aatcatatgt gtatgtagac catctgtcaa agaggatagc aattctgcat
     4081 tggaaacttt taatttagca gtaaaattat caatgtcttt ttggctttga atgtttaagg
     4141 gagcagtcag catcccataa atagggtgct ctacctccac attcataatg attccttgat
     4201 accgcaaaat aatttccagt tcttcttctg tctgttctat gccatgctga caaaccaatc
     4261 tagctgtgaa ttgatgagta cttagagcac tggacataag gtatcctttt ggggtagaaa
     4321 taatatcaca ttgttgtgca cgcaataagg caatatctcc aacaatcacc tgcctactaa
     4381 cactaaaaat ctttgctaat gtgcttgccg atatagcttc ttttgttcct tttaaagtcg
     4441 ttaaaatatt ttctcgacgc tcctgtgcct tcatataaca aattccttct ttggtgttat
     4501 ttcacaatta aaaactggct agaatatcta accagttagc ctcaatcatt tcgtgcaaaa
     4561 agacgaagaa tatttaagaa tagattaata aaatcaagat aaaggcttag tgccatcgaa
     4621 actgcccaac catctgcgac ttggccgcct tggctattgt agacatattt aatcatttgg
     4681 ttatcgtaag caatcaatcc agaaaagatg atgacacata cgatactaat gatataactc
     4741 atacccccac taccgataaa taaattaaca agacttgcaa ttaaaatccc aattaaagca
     4801 gccattaacg ctttacgcat acctgataaa tcttttttag tcttagctcc aatcaaagcc
     4861 attgcaaaga aaacagccgc agatgttata aaagcttgaa gaactgtagt ttgcgtgtac
     4921 cttgcgataa tgaaacttaa agtaaaccca ttcaaagctg aataaatcaa aaataatggt
     4981 aatgcagctg gtgtgtttcg acgcgccgct ccactagcaa caaagactaa aaccaattct
     5041 aagatgattg ctccaaaata caaccctggc attttttgca tgatggcaat catattttgt
     5101 ggaaacatat acagcataat cgcagaaaca gcagctgaca aacctactcc tatccctaca
     5161 agaccgtaaa tcttagcaaa aaattgattc agccctgaat cagattgtgt gtaaatgacg
     5221 ttatcattca tatgtatttt cctattctaa attttaatga ttatgactac ctaagaattt
     5281 gcgactaaaa cggataggtt tcttaaaaat acgtttgagt ggacgagaag cctctcgtac
     5341 tgcccaatac ttatcaacca tagtcacaat ataactttct ttataacgtg gcggtgcaat
     5401 agtcgctccc cacatgtgct tcaaaataat atcttcttct ctagcgttta aatcagttaa
     5461 ttttcgcgca ttacgaaccg ctatccttgg atgaacccag gcatggctct tgttaaattt
     5521 cgttacacgc caatcataat aaaagaaatc atgtagtaaa ccacctcgag cagtactact
     5581 cgcatcccaa ccaaacttct tagcaatttt ataacttgta taactaacat ttatagaatg
     5641 ctctaagcga gttgagtgat gatgttggac aatagcttcg agtttttgga aacgtggatg
     5701 gtcaataagg tgaccgacca aatccatgaa gtccttatcg tcttgatact ttttcattat
     5761 aatcacccct ctggggatat tataacatat gaacttaaat taagctgaaa atgactactc
     5821 ccgcagctac tgctacatta agactttctg cttgtccagg catttcaata tgtgctaaaa
     5881 catctgcttc ttctgtcata gttttggaaa ttccctgtcc ctcatttccc atgattaaag
     5941 caaaataatc atatttaggg agattcttat aatttaccga ttgttcagat aaagttgtcg
     6001 caatcatagt tatcccttct gctttacaaa agttaacaaa ctgctctatt tctaccctaa
     6061 atactggtaa atgaaagtga ctgccttgca tagaacgaag tgttttttgg ttatacaaat
     6121 cagcagattt ctgagataaa aaaacagcat caaaattcgc tgcatctgca gttcgaacca
     6181 ttgttcctag gtttcctgga tcctgcacat cttctaatac aagaaaacgt ccttttttaa
     6241 tatctgtcca tcttgtctct tgaaaggcaa tctcagctac aatcccttga ggagtctgtg
     6301 agtcagttaa ttctttcaaa acatcatctg taacaacaat agctctctcg ggttttctca
     6361 aacgatctat tgccgtttct gtcacaaaaa tatttaaaaa ttgtgcacca tatttttcag
     6421 cttcctcaaa taaatgccag ccttcaataa gatatgaaga tttccgatat tttttttgta
     6481 acaacttttt cgttttttta atgagattat ttgattttga agttataata gtagtcataa
     6541 acctattata tcagaaaaga ggtcaagtta tgaaaaaagt tcatttaatt gtctcagggc
     6601 gcgttcaagg agtcggattt cgctatgcaa cttattcctt agctttagaa ataggtgata
     6661 tttacggtcg cgtttggaat aacgatgatg gaactgttga aattttagca caatctacag
     6721 attcaaataa aatgacacaa tttattcaga aaattcgaaa aggaccttca aaatggtcta
     6781 aagtaactta tgttgatatt aaactagata acttcgatga ttttaatgac tttaaaatga
     6841 gtaattaaaa atcattctgc ggaagtattg tttaattctt aaaaaaaaag taaaatagat
     6901 aggtatatta aaaattagag gaactaaaaa cattgaaaaa aacattgaaa cgtatcttat
     6961 tctctagctt atctttgtct atgttattat tattgacagg atgtgttagc gttgataagg
     7021 ctggaaaacc ctatggagtt atctggaata ctcttggagt gcctatggcc aacctcatta
     7081 cttatttcgc tcaacaccaa gggcttggtt ttggtgtcgc tatcattatt gtaaccgtca
     7141 tcgtacgtgt cgttatctta ccacttggac tttatcagtc ttggaaagct agctatcaag
     7201 ctgaaaaaat ggcatacttt aagcctttat ttgaacctat taatgaacga ctacgcaatg
     7261 ctaaaacaca agaagaaaaa ttagcagctc agacagaatt aatgacagct caacgtgaga
     7321 atggccttag catgtttggc ggtattggct gcttgccatt actcattcag atgccatttt
     7381 tctcagctat tttctttgct gcacgctata ctcctggagt ctctagtgca accttcttgg
     7441 gacttaactt aggtcaaaag agtctgacac ttactgtcat tatcgccatt ctttattttg
     7501 tacaatcttg gttgtcaatg caaggtgttc cagatgaaca acgtcagcaa atgaaaacaa
     7561 tgatgtatct gatgccaata atgatggtat tcatgtctat tagtttacct gctagtgttg
     7621 cgctttattg gtttattggt ggtattttca gtattatcca gcagctagtt acgacttatg
     7681 tacttaagcc taaactacgc cgaaaagtag aagaagaata tactaaaaat cctcctaaag
     7741 cctacaaagc taataacgca cgaaaagatg ttacaaattc aactaaagcc actgaatcta
     7801 atcaagcaat tattacatct aaaaaaacaa atcgtaacgc cggaaaacaa aaaagacggg
     7861 gataaaacaa gcatactaaa taatggtgtg ttgtcctaaa cagttaaaga ttattatgta
     7921 attcaagtaa aaaagactta gttctatgtc ctacatagct aagtcttttt agttttgcct
     7981 aacttttcat ataaaaagca ccctaaaagt cagataaaaa tctagctttt aaagtgcaag
     8041 taatctagct aacagactac cttattaata aagtaatcgt ttttcccacc agtcttgaat
     8101 aattgcataa agcgtacaaa ttacccaata gataagggct actaagatat aggcggtcat
     8161 atagtcggag taggttcccc caactatttt agcattatta aagatatcag gtaccgtaat
     8221 catcgcagtc aatgaggaac tcttgaccat atctagcaaa acattactta aagaagggag
     8281 tgcaattctt gttgcttgcg gaataataat gcctctataa atcgtcggtg tttttaaccc
     8341 taaagctcta gcagcttccc actgaccatg atcaacagct aaaagagctg ctctgataat
     8401 ttctgatata tatgcactag acatcatggt aaaacctacg atagaagcag tcacagcatc
     8461 taactgtatc cctataaaag gcaaaccaaa gtagataaga aagagtagaa ccattaaagg
     8521 aatcccacgc attaaggaaa tatgaatatt agctagatac ctcagtaagc ggaaatgaga
     8581 catcttcatt agagctacaa agaatcctac gatagctcct aaaataaagc caaccaagga
     8641 taatccaatg gtatatggta acccctgaat aacacttgga aaagccttaa cagctagttc
     8701 caaattaaaa atggcatccc agtttatcac ttatctactc ctctcgaaat tttttattta
     8761 actttaatta ctggaatttt atccgttcca gcaggttctt ttgtgagatc ttgaccagca
     8821 taatattttt cagagatttt tttcaaagaa ccgtctttct tcatatcctt gatagctgca
     8881 tcaatttttg ttttgaggct cttatctttt ttactcatca caataccttg ctcagtcgga
     8941 ttatacttca catcacccat ttttactttg atatctggat attgttttgt tacatatttt
     9001 acagcaatga cttgtgtgta gtaatcattc ggaatgaaat ctgtacgtcc agttgataca
     9061 tctcgaaggt aaacgtcatt agttacattg tcataaataa caggttcagc accttgctgt
     9121 tttgcaattt tcatatattg cgttccagcg ccaccaccag cttttttacc tttccagtca
     9181 gataaatctt ttgctgtaat ctttgaagaa ccatctgcac gtacaatcat acctccaaca
     9241 gaatatttat aaggttgaga gaaattgtat tttttcaaac gctcgggagt tgtgtcaaag
     9301 ttattgacag caacatccac cttaccacta tctacagaag taaatgattc agcaactcca
     9361 acttcaacaa acttaacttt tatcttaagt tttttagcaa tcgctttcat catatcaatc
     9421 tcataaccag tcaatttttt atggtcatca taataacttg ttggataaag cgttgcaggg
     9481 gtagcaacct taaggacacc atctttttta atattgtccc atgttgtttc tttgctagat
     9541 tgaccatttg agcaagctgc taatgtcatt gcagataaac cgactaaacc aagtaaaatt
     9601 gtctttttca taagtactcc tgtagattaa attatattaa aatggtttgg cgtactctat
     9661 cgaaaattaa atttaatgaa aattaataca taaatttacg ataataatac aaccattgtt
     9721 aagagtacca tattgtatcc gttttcgcaa aaatattgtt tacttttcaa attaatggta
     9781 aacaggccct ttgaattgct tctctttttc aaacaactct gcaacagcaa gcaataaatc
     9841 ttctctccct ttagccgctg tcaattgtac acctaaaggt agcccctctt tagttagatg
     9901 agttggtatg gctaaagaag gttgccctgt caagtttggc tgatgtgtaa aaggtgtcca
     9961 ggccattgag tcttcaaaca ttttccaaat taaatcctgt tgttcactta ctgagaattc
    10021 tcccatatgc ctcaattgtt tctggagtgt ctcgtctaaa tcaaattgtc catgaaaagg
    10081 agctggttga ttagttgctg ctgttaaaat taaatcataa ttttcatgaa acctcgccat
    10141 ggtagctgca aattgatccc atgaatctaa gagtttactg taatctttag ccaataccct
    10201 ttgtccactc tgatacatag cccaggtcat taattccata tcgctaaatt ccattgaacg
    10261 ccctaaactc ttttcgatgt catcaaacat tgctgctgtt tcaacgctat tcatcataca
    10321 ataagttttc atagaatgaa taccatctaa aggttgttct actaattcta aatgattccc
    10381 tttttgagac agaaaatctg cggcttcttt aatagctgct ttagcctcac ttgatacctt
    10441 tgtttttaag ggagaatcca tcaatactgc tatttttagg ggtttactaa cgctaaactc
    10501 gaaaagactc tctttgctga gttttttcag gggaaatggg ctttctactt gatatgattg
    10561 taaataatat aatagtcgtt ttgtatctct aactgacttt gttaaagcaa aatgactaga
    10621 agccccttgc caacctctat aggatgaagg tccaacaggt atacgtcctc gactcggttt
    10681 taaaccaatc aaaccattaa aacttgctgg aattctgatt gaccctcctc catcactagc
    10741 tcctgcaatt ggaaccattc ctgaactaac agctgcggct gcaccaccac tagagcctcc
    10801 agcatttctt gaatggtcaa atggaagatt aacattcccg tgaagttgac catcactaat
    10861 attcttaaaa ccaaattcag gggtatttgt tcttcctaaa ataataaacc ctaatttttc
    10921 aaaggactgc actaaatagt ccgtttgttt agcatgatag tgtttaaata attgtgaacc
    10981 tgaggttgag agttgtcctt tttgattctg tcccaaatct ttcagaagaa taggaactcc
    11041 tgcaaaagga gcatttgatt catttgcgta tttggctgct tgacgcgcct cattatattg
    11101 cttgctgaca acagcattaa cgcttacatt ctgttcctcg atcttgtaga ttgcttgctc
    11161 tactaattct tgcgacgaaa tcttatgctg ttttatcgct tgtaccatag cggtagcatc
    11221 tttaaaaacc atataaacac ctcataaaat gtatttttat tataagataa caaaaaatac
    11281 ttaaaaaaat agaataacgt ctagactcta gatattattc tatgatttat actattaatt
    11341 tgttttttca acactaatga tttcaacttg atagctacca gcaggtgatt caattgtagc
    11401 taagtctcct gtttttttac caattaaagc atgagcaatt gggctttcat tcgaaatttt
    11461 tcctgaaaaa atgtcagctc cagcagcacc aacgatatgg taagtatctt tatcatttgt
    11521 accaacttct tggacaagga cagttttacc aattgcaacc tcatctttag caactgcatc
    11581 actatcaata atttcagcat aacgaatttt tgtttctaaa atttgaattt gaccctcaac
    11641 aaatgcttgt tcgtcttttg ctgcatcata ttcgctattt tctgataagt caccataaga
    11701 acgagcaatc ttgatacgct ctacaacttc tggtctacga actaatttta actcttcaag
    11761 ttctttttca agttgatctt tttccacttg tgtcattggg taagtttttt ctgccatttt
    11821 atttctcttt tcttttcatt ttttcaagtc aaagatataa acatgatgac tcgcaccatg
    11881 tttataagct tagttaattt gactattgat atatttttca acgttagcct tgtgtgtttc
    11941 aaaatcttta gcgtagtata catttccggt cttcacatca gcgacaaaat aaaggtaatc
    12001 tgttgaagca ggttttatag tcgcttcaat tgctgagata cttggactat caactggtcc
    12061 aggcatcaaa ccagtattag tatagatgtt gtatggtgat ttaattgaag tattaatttg
    12121 agcatcttca gctaaacttg ttttatcacc taatttcccc atagcataca aaattgcaat
    12181 gttactttga agagcttgac cggctgataa tcggttataa aagacacttg ctattttacg
    12241 gcgatcttcg tctgttgatc cctctttttc aacaagagat gataatgtca aaacatcatt
    12301 tacagacata ttcttcgctt taatagtgtt atagtaaggt gccatcttag tattcattgt
    12361 tgaaatcata tcttcaacaa gtccttctaa ggttgtatct ttataatagt tgtaggtagc
    12421 tgggaataaa tatccttcca attgataaat agcttttgat ttatcaggta aacttcctag
    12481 taaatttgga tatttagcta ccattttttt aataaacgtt tcatcttgaa caagtttcaa
    12541 gaaatcatca gctttataag gtgtacttgt agtctttgta tcaattttat tagactcaat
    12601 agctttagca atctgtttaa tagtgtaacc ctcggtaatt aatatcttac ctaaagctgg
    12661 ttttgtcggt tctgcagttc cgcctttttc caattcagca gcaatctgat ctaaagtcat
    12721 acttttttta agattgtagt atccactttg aaaattacta tagtttttga acttggaata
    12781 gtaattaaag actgtggcgc tcttaatgac tcctgctttt tctaaaattt gaccaattaa
    12841 tttattccca gatcctatcg gaatttcaac ttgtacaaat ttatttgaat tggaatcaac
    12901 aggtttaata gcactatcta caaaacgata acctacaaat gctgtcaaga gcaatacaag
    12961 tatgataatt gatatcagaa tacgtgcaat tcgttttgcc acattatctg ctttctgacg
    13021 tctttcacgc ttgttatgtt tgttcttggc tgacaaagct ataccctctt cttcttttga
    13081 caaatcctct actcggatag gttcctttgc tacgggctct aactctgata tctcaccaag
    13141 ttttacattt tcaccttctg taggaatatc tgttgctctc acaagaaaga tttcttccgt
    13201 ttcttgatta ggcacagcag ttgcaacatc agtcttatct gcttttaaat ctatgtcagg
    13261 tacattttca gttgtcctaa attcactaga tttggagccg ctatcaagct ctcttgaagg
    13321 ttccatccct tgattgataa cttgttcttc ttgggaagct aatgcttgtt ccgcctgacg
    13381 tctttcttct tctcgagcag cttctgccag aagtttcgcc tcaatccgag ccttctcttg
    13441 ctcttctaag cgttgcttag tctctagcgc ctttgctcta gcttcttgtt cgtctttcaa
    13501 acgttgcgct tcataatctg ccatcaattg tgctgttctg cgagcagctt ctttggcttc
    13561 ttgctctttt tgatagagtt cttcttcacg caactttctc aaacggttag cttcttctaa
    13621 ctctgccaga atttgttctt taaaactttt ctgatcatgg ttagaatgct gatcgtcatt
    13681 aaattcggtc aaagagattt cctcctaata ataatcacag gcaattatat cttaaatagt
    13741 ctataaaatc aacaaaactt tgacaatgtt atctactttt aacaaattga tcgtgttgga
    13801 ttattccaaa tcatattata ccaaagcgaa ccaccatgtt tggagtcaga ttctccctca
    13861 tctttaaatc cattcatttc atagaaggaa attaaatcat catggcaagt taaacttata
    13921 ccatctcgtt cttgagaaac aaccaggtct ttcatcgctg ctaataatgc tgtaccaatc
    13981 ccctgacctt taaaatcagg atgtattgat agactagtaa ttcctatgaa accacctacc
    14041 cttacaggga attcagaaac tttatggaat aaatcgtccg ttaagtaccg acctttaata
    14101 accggtcctt caatgtaacc tgctaaacga ccattaatct ctgcaactaa aaaagtatca
    14161 gtcattatcg ttaaacgttc ttgcattgct tttttactcg cagcctctgc ttctgaaaaa
    14221 tttatttgtt caatactaat gacttgctgt aaatcctcta ggcaaccatt tctaataatc
    14281 atactcatct ccttaatttc tttatgaaat tctcttgata aagtcagata gaggactgag
    14341 aaacttctct cagcccttac catcaactta ttgtgtattt ttagttaggt tagctaataa
    14401 ttcttcaaaa gagcgctcat acaattgaat gtctccagca cccataaaga catagacagc
    14461 attatcatga ttgagtaaag gcgagacatt ttcgactgtc actaaatctg agtgtttgac
    14521 aatcttagca gctaaatctt ctaccttcac ctcaccatta tctacttctc tagcagaacc
    14581 atatatttga gcgagataaa cgctatccgc ttgactcaag gcatgggcaa attcgtctaa
    14641 aagagctatc gtacgagtga acgtatgcgg ttggaaaata gctacaattt cttttgacgg
    14701 gtatttttgt cgagcagcat ctaatgtcgc aataatctca gtaggatggt gagcaaagtc
    14761 atcaataatg acagtatcgt caataatctt ctcagtaaaa cgacgcttta cccctgaaaa
    14821 tgtcttcaaa tgctcagcta ctaatgccat atcaattccc attatgtaaa ggttagcaat
    14881 aacagcagtt gcatttaaga tattatgttt accgtatgct ggtacatgaa actgaccaat
    14941 ttcttcttgg ttatagaaaa ccttaaagtc agaaccatta acagttcgag tgatgtcttt
    15001 tgctataaaa tcatttgaat cttcaaaacc ataataatat attggtgcct cagaagtgat
    15061 ttcatgaagt tttggatctt ctccataaat gaataaacct ttttgaactt gcttagcata
    15121 gtcattaaag gcattgaata cgtcctctaa gcctgtaaaa taatcaggat ggtcaaaatc
    15181 aatattggta ataattgagt attctggatg gtacggcata aaatgacgtt cgtattcatc
    15241 agcttcaaac acaaagtaat tagcattagc agaaccacgt cctgtaccat ctccaattag
    15301 gaaagaagtg tctgtaatat tttttaaaac atgagctaat aaacctgtcg ttgaggtttt
    15361 tccatgtgcc ccagctacac ctagactagt gaactgacgc ataaaatctc cgagaaattc
    15421 atgatatcgt ttaaaatgat agcccttttc aataacataa gccaactctt cattgttatc
    15481 tggacgaaaa gcatttcctg caataatctc taaatcctca ctgatattat tcggtgagaa
    15541 aggtaatata gttacacctg cttgctctaa accacgttgg gtaaaataat atttgtcaac
    15601 gtcacttcct tggacgttat gtcccatttg atgaagcatc agtgctaggg cactcattcc
    15661 ggatccttta ataccaataa aatgataagt ttttgacatg gtctctcctt aattaatgtc
    15721 tttaaaacgt gtcagattta attcttgagc cttggctctt tctttatgaa actgattgtc
    15781 ttgtttattg taaacttgac tgcgtttcaa aaaatcaaaa ttatttttct ttgtttttgg
    15841 gttcggtgta ttatcaggtt cttgaaatat gattggaatt tctgctaata tatagctttc
    15901 ctgctgtaaa cgatcagaaa gatgacttag ttcagtcacc tttcgctgag tttgtttttt
    15961 cggtactgtt gccttcaatt gttgctgaaa agcttccttt gatggcgcct taaattcctg
    16021 tttcgcatag taagcttgtc gtttctcccg aacagacctc tttgcctctt gtcgagattt
    16081 gctcaactct gtcgcttttt ttacaaaact ctgcggtggt acaggatcat tttcaattac
    16141 cttaggatgt gatatttctt catgttcata attgcttttg accatgcttt gatatgtttt
    16201 ttcttgataa aagtctcgaa tgttgttaat taaatcttca ttatcataaa gattcatctt
    16261 tacaagcgga gacattactg gttcatcatc tgctatcaac ggaaatcgtc tagagcctct
    16321 attcacatta tcacttcctt tctacaacag acctattata aactaatttt tctgtttttt
    16381 caaagttttc aactaagtaa agcttatcag a
//
LOCUS       DATRMA010000041        16335 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.041, whole genome shotgun sequence.
ACCESSION   DATRMA010000041 DATRMA010000000
VERSION     DATRMA010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16335
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.041"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            complement(<1..909)
                     /gene="brnQ"
                     /locus_tag="VBC28_001265"
     CDS             complement(<1..909)
                     /gene="brnQ"
                     /locus_tag="VBC28_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HEO6815250.1"
                     /translation="MVKKGFLTGLLLFGIFFGAGNLIFPPALGVASGQDFWPAILGFC
                     LSGVGLAIITLLLGTLTNGGYKTEMSEKFSPWFSLTFLVILYLTIGPLFAIPRTATVS
                     FEIGVAPIVGHSPIALLCFTACFFAAAYYLAIRPNGILDSVGKILTPVFAFLILSLVV
                     VGAIAYGNLESAKASADYAGKAFGSGVLAGYNTLDALAAVAFCLVATETLKKFGFKTK
                     KEYLSTIWIVGIVTSLAFSILYIGLGFLGNKFPVPADILADPNVNKGAYVLSQASYKL
                     FGNFGRYFLSIIVTLTCFTTTVGLIVS"
     gene            complement(1105..3102)
                     /gene="metG"
                     /locus_tag="VBC28_001266"
     CDS             complement(1105..3102)
                     /gene="metG"
                     /locus_tag="VBC28_001266"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEO6815251.1"
                     /translation="MTEKKSFYITTPIYYPSGKLHIGSAYTTIACDVLARYKRMMGFD
                     VQYLTGLDEHGQKIQQKAEEAGITPQEYVDGMAESVKTLWELLDISYDKFIRTTDTYH
                     EEAVAKIFEQLLAQGDIYLGEYTGWYSVSDEEFFTESQLAEVYRDENGNMIGGVAPSG
                     HEVEKVSEESYFFRMSKYADRLKAYYAEHPEFIQPDGRMNEMLKNFIEPGLEDLAVSR
                     TTYTWGVQVPSNPKHVIYVWIDALMNYISALGYGWSDDLSQYHKFWPADIHMIGKDIL
                     RFHSIYWPIMLMALDLPLPKRLVAHGWFVMQDGKMSKSKGNVVYPEMLVERFGLDPLR
                     YYLMRSLPVGSDGTFTPEDYVGRINYELANDLGNLLNRTIAMVNKYFDGEVPRFAVAT
                     DFDADLASVATDSIENYHKQMEAVDFPRALEAVWNLISRTNKYIDETAPWVLAKDETD
                     RDKLAAVMSHLVASLRVVAHLIQPFMMETSDAIMEQLGLGATFDLEKLTFADLPEGVR
                     VVAKGSPIFPRLDMEDEITYIKEQMNAGKAPVEKEWVPEEVELTSSKGQIKFEDFDAV
                     EIRVAEVIEVEKVEGSDKLLRFRLDAGDEGHRQILSGIAKFYPNEQELVGKKLQIVAN
                     LKPRKMMKKYVSQGMILSAEHDGKLTVLTVDSAVANGSIIG"
     gene            3245..4120
                     /gene="tehB"
                     /locus_tag="VBC28_001267"
     CDS             3245..4120
                     /gene="tehB"
                     /locus_tag="VBC28_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase TehB"
                     /protein_id="HEO6815252.1"
                     /translation="MVNTKTLLPYKTMPVWTAQSIPKAFLEKHNTKEGTWAKLTILSG
                     SLVFYQLSPDGEEISRHIFDASSDIPFVDPQVWHKVSPNSPDLSCYLTFYCQKEDYFH
                     KKYGLTRTHSEVIASAPLLSEKSNILDLGCGQGRNSLYLSLLGHQVTSVDSNGQSLVA
                     LENMALEEELPYNIKRYDINTAAIEGHYDFILSTVVFMFLNPDCISDIILQMQSHTQI
                     GGYNLIVSAMDTAENPCPLPFPFTFKEGQLKSYYNDWEIIKYNENLGELHRVDENGNR
                     LKLQFATLLARKLRS"
     gene            complement(4278..4973)
                     /locus_tag="VBC28_001268"
     CDS             complement(4278..4973)
                     /locus_tag="VBC28_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HEO6815253.1"
                     /translation="MSKKSHRQYQIYEGLRCAVALCFISGYINAFTYVTQGKRFAGVQ
                     TGNLLSFAIHLSNKHYSQALAFLLPIMVFMLGQSFTYFMNRWANKHQLHWYLLSSFAL
                     TQVAIVTIILTPFLPSSFTVAGLAFFASIQVDTFKSLRGAPYANMMMTGNIKNAAYLL
                     TKGLYEKNSDIFLIARNTIIIIGGFIFGVVCSTYFSSKLGEWSLSLILIPLLYVNLLL
                     GHEFYNLQVERHS"
     gene            5309..6526
                     /locus_tag="VBC28_001269"
     CDS             5309..6526
                     /locus_tag="VBC28_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEO6815254.1"
                     /translation="MHSTIYKCHHCRYLIYKSFKDIFPFALIFSWIEILQKLVISDDS
                     FLAQTTKSSIYKNEWLYTVSNTLYDLSHLLIYFMASYFVASLVWRDLSNQTKESFSAS
                     LLGFATVWLLLTSVVPGTDYHYPSQPIWLMLALISYLFIFLTRLLQNRHHIFQYSMLA
                     ILLLISSILSHILYRYPNIDLEYLIQNHFSQWLGDGPHQLIPIMLWSILAIFLTLSGL
                     FVPDILFRPYSYFSVVSENLNAALSQHTDKIPYLYTFYTVKNSFAMFGGIGILLSLFL
                     AVLYESRKLQSKNYYKLTLLTLTPLIFDQNLPFLVGLPVILQPILFIPMVLTTIFAEA
                     FGALMLYLKFVDPAVYTVPSGTPSLLFGFLASNGDWRYLPVTAIILVVGFFIYRPFVK
                     IAFAKEEQYEKIT"
     gene            6510..7352
                     /locus_tag="VBC28_001270"
     CDS             6510..7352
                     /locus_tag="VBC28_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEO6815255.1"
                     /translation="MKKLLRWLPPVLFIIILIGMTILGKSYINKVTAHKIKLYNSRMT
                     PTILIPGSSATQERFNSMLAQLNQMGEKHSVLKLTVKKDNSIIYNGQISGNDHKPYIV
                     IGFENNEDGYSNIKKQTKWLQIAMNDLQKKYKFKRFNAIGHSNGGLSWTIFLEDYYDS
                     DEFDMKSLLTMGTPFNFEESNTSNHTQMLKDLISNKGNIPSSLMVYNLAGTNSYDGDK
                     IVPFASVETGKYIFQETAKHYTQLTVTGNNATHSDLPDNPEVIQYVAEKILKNEKGKL
                     PKPH"
     gene            7429..8256
                     /locus_tag="VBC28_001271"
     CDS             7429..8256
                     /locus_tag="VBC28_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEO6815256.1"
                     /translation="MKLISWNIDSLNAALTSESTRALMSRQVIDTLVAEDADIIAIQE
                     TKLSAKGPTKKHLEVLETYFPEYDLVWRSSVEPARKGYAGTMFLYRKGLNPIVSFPEI
                     DAPTTMDNEGRIITLELENCYITQVYTPNAGDGLKRLADRQIWDIKYAEYLATLDSQK
                     PVLATGDYNVAHKEIDLANPSSNRRSAGFTAEERQGFTNLLAKGFTDTFRYLHGDVPN
                     VYSWWAQRSRTSKINNTGWRIDYWLTSNRVADKITKSEMIHSGDRQDHTPIILEIEL"
     gene            complement(8296..8652)
                     /locus_tag="VBC28_001272"
     CDS             complement(8296..8652)
                     /locus_tag="VBC28_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000287944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HEO6815257.1"
                     /translation="MYTFYEYPKCTTCRSAKKELTELGLTFEAIDIKSNPPKVSLLKE
                     LLENSPYDLKKFFNTSGNSYRELGLKDKFDDLTLDQALDLLASDGMLIKRPLLVKDNK
                     ILQIGYRTKYKDLNLV"
     gene            complement(8654..9130)
                     /locus_tag="VBC28_001273"
     CDS             complement(8654..9130)
                     /locus_tag="VBC28_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEO6815258.1"
                     /translation="MLYQEFYQSPLGEIRLLADNLGLSGLYFVGQKYDMLAVNQEEIV
                     NMSNSYTLLGKKWLDAYFSQQNLPSIPLSLRGTAFQTRVWQELQKIPFGDTKTYGELA
                     KELNCQSAQAVGGAIGKNPISLIIPCHRVLGRYGQLTGYAGGLERKSWLLEYEKEK"
     gene            complement(9185..10365)
                     /locus_tag="VBC28_001274"
                     /pseudo
     CDS             complement(9185..10365)
                     /locus_tag="VBC28_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019321778.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
     gene            complement(10428..10976)
                     /locus_tag="VBC28_001275"
     CDS             complement(10428..10976)
                     /locus_tag="VBC28_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001167084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEO6815259.1"
                     /translation="MQIRLAFPNEIDQIMLLIEEARAEIAKTGSDQWQKEDGYPNRND
                     IIDDILNGYAWVGIEDGMLATYAAVIDGHEEVYDAIYEGKWLHDNHRYLTFHRIAISN
                     QFRGRGLAQTFLQGLIEGHKGPDFRCDTHEKNVTMQHILNKLGYQYCGKVPLDGVRLA
                     YQKIKEKGETSIYREIDERNPM"
     gene            complement(11044..12136)
                     /gene="serC"
                     /locus_tag="VBC28_001276"
                     /pseudo
     CDS             complement(11044..12136)
                     /gene="serC"
                     /locus_tag="VBC28_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587955.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
     gene            12269..12904
                     /locus_tag="VBC28_001277"
     CDS             12269..12904
                     /locus_tag="VBC28_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HEO6815260.1"
                     /translation="MILREFCAENLTDLTRLDKAIISRVELCDNLAVGGTTPSYGVIK
                     EANQYLHEKGISVAVMIRPRGGNFVYNDLELRIMEEDILRAVELESDALVLGILTSNN
                     HIDTEAIEQLLPATQGLPLVFHMAFDVIPKSDQKKSIDQLVALGFTRILLHGSSNGEP
                     IIENIKHIKALVEYANNRIEIMVGGGVTAENYQYICQETGVKQAHGTRITQ"
     gene            complement(13174..14043)
                     /gene="rsmI"
                     /locus_tag="VBC28_001278"
     CDS             complement(13174..14043)
                     /gene="rsmI"
                     /locus_tag="VBC28_001278"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000425856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEO6815261.1"
                     /translation="MEMQVQKSFKSNIHYGTLYLVPTPIGNLDDMTFRAIRILREVDF
                     ICAEDTRNTGLLLKHFDITTKQISFHEHNAYDKISGLIDLLKEGKSLAQVSDAGMPSI
                     SDPGHDLVKAAIEGDIPVVSIPGASAGITALIASGLAPQPHIFYGFLPRKKGQQITFF
                     ETKQDYPETQIFYESPFRVSDTLKHMKEIYGDRQVVLVRELTKLYEEYQRGTISQLLE
                     HIEKVPLKGECLIIVDGKRDTERVKDSSQQDPLVLVKEYIANGDKTNQAIKKVAKEFN
                     LNRQELYASFHDL"
     gene            complement(14043..14369)
                     /gene="yabA"
                     /locus_tag="VBC28_001279"
     CDS             complement(14043..14369)
                     /gene="yabA"
                     /locus_tag="VBC28_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HEO6815262.1"
                     /translation="MDKKDLFDAFDDFSQNLLVGLSEIETMKKQIQKLLEENTVLRIE
                     NGKLRERLSVIEAETETAVKNSKQGRELLEGIYNDGFHICNTFYGQRRENDEECAFCI
                     ELLYRD"
     gene            complement(14399..15262)
                     /locus_tag="VBC28_001280"
     CDS             complement(14399..15262)
                     /locus_tag="VBC28_001280"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEO6815263.1"
                     /translation="MDLKRTQPKLLEKFNTILQSDRMSHAYLFSGNFASLDMALYLAQ
                     SQFCEKRQSGLPCQECRACRLIANGEFSDVKIIEPQGQLIKTETIKELTKDFSRSGFE
                     GKSQVFIIKDCEKMHVNAANSLLKFIEEPQSSSYVILLTNDENNVLPTIKSRTQIFRF
                     PKQLDMLVHQAEQAGLLKSQASLLAQVADDPKHLEILLTNKKLLDYLNLSQQFVTTLA
                     KDRQTAYLEVSRLTSQVVDKNDQAFVFQWLTIMLAKEGQLYDLENTYRAQQMWKSNVS
                     FQNSLEYMVLS"
     gene            complement(15282..15917)
                     /locus_tag="VBC28_001281"
     CDS             complement(15282..15917)
                     /locus_tag="VBC28_001281"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000715594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEO6815264.1"
                     /translation="MKKGLMISFEGPDGAGKTTVLEAVLPLLREKLSQDILTTREPGG
                     VTISEEIRHIILDVKHTQMDKKTELLLYMAARRQHLVEKVLPALEEGKIVLMDRFIDS
                     SVAYQGSGRGLDKSHIKWLNDYATDSHKPDLTLYFDVPSEVGLERIQKSVQREVNRLD
                     LEQLDMHQRVRQGYLELADSEPNRIVTIDASQQLDEVIAETFSIILDRINQ"
     gene            16126..>16335
                     /locus_tag="VBC28_001282"
     CDS             16126..>16335
                     /locus_tag="VBC28_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000160532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO6815265.1"
                     /translation="MTKHKHLTLLDRNDIQSGLDRGETFKAIGLNLLKHPTTIAKEVK
                     RNKQLRESTKDCLDCPLLRKAPYVCN"
BASE COUNT         5255 a         3134 c         2581 g         5365 t
ORIGIN      
        1 tgacacaata agcccaactg ttgttgtaaa gcatgtcaat gttactataa tactcaagaa
       61 ataacgacca aaatttccaa ataatttata agatgcttgt gataatacat aagctccttt
      121 attaacattt gggtcagcta aaatatcagc tgggacagga aacttattcc ctaaaaaacc
      181 taagccaata tataaaatac taaatgctaa actagtaaca attccaacaa tccaaatggt
      241 tgataaatat tcttttttag ttttaaaacc aaacttttta agcgtttctg ttgctactag
      301 acaaaaagca actgctgcaa gtgcatcaag tgtattatag cctgctaaga caccacttcc
      361 aaacgctttt cctgcatagt cagcactcgc cttagcactc tccaagtttc cataagcaat
      421 agctccaaca acaaccaaag agagaattaa aaaggcaaaa acaggcgtta agatttttcc
      481 aacactatct aatataccat ttggtctaat agcgagataa taagctgcag caaaaaaaca
      541 ggctgtaaaa cacaaaagag caatcggact atgccctaca attggagcaa ccccaatttc
      601 aaaagaaacc gttgctgttc taggaatagc aaaaagtgga ccaatagtta ggtagagtat
      661 aactaaaaat gttaaagaaa accacggact aaatttttcc gacatctctg tcttataacc
      721 accattagtt agtgtgccta gtaggagggt aataatagct aatccaactc ctgacaaaca
      781 aaatcctaaa atcgctggcc agaaatcttg tccagatgct acacctaatg ctggtggaaa
      841 aatgaggttc cctgcaccaa agaagatacc aaataagagt aatcccgtta aaaagccttt
      901 ttttaccata agcaaccatt agtgtaaata atctatttac accctttcca taaattctcc
      961 ttatcaaatt ctatctaaat gcttagaatc gatgtctttt attatatcaa aaataaaaat
     1021 aaattctagg tcattttcat aattttccaa ctattcagtc acttttttct tgaaaacaaa
     1081 aaaagagcat aatgctcttt tcgattaacc aataattgat ccatttgcca ctgctgaatc
     1141 gaccgtcaac actgtcaatt taccatcatg ttctgctgag agaatcatgc cttgactgac
     1201 atattttttc atcattttac gtggtttgag gttagcaaca atttggagtt ttttccctac
     1261 taactcttgt tcatttgggt aaaattttgc aatccctgat aagatttgac gatggccctc
     1321 atcaccagca tctagacgga aacgaagaag tttatcagaa ccttccactt tctcaacttc
     1381 aattacttct gcaacgcgga tttcaacagc gtcaaaatcc tcaaacttga tttgaccttt
     1441 gcttgatgtc aattcaactt cttcaggaac ccactccttt tccactggtg cttttccagc
     1501 attcatttgt tctttgatat aagtaatttc gtcttccata tccaaccttg ggaaaattgg
     1561 tgaacctttt gcaacaactc gaacaccttc tggtagatca gcaaaagtca atttttcaag
     1621 atcgaatgtt gcccctaaac caagttgttc cataatggca tcggaagttt ccatcatgaa
     1681 cggttgaatg aggtgagcaa caacacgtag actagcaact aaatgactca ttacagctgc
     1741 taacttatca cgatctgttt cgtcttttgc aagtacccac ggtgctgttt catcaatata
     1801 tttgttagtt cgtgaaataa gattccatac agcttcaagg gcacgtggga aatccaccgc
     1861 ttccatttgt ttatgatagt tttcaatact atctgtcgca acagaagcta aatcggcatc
     1921 aaagtcagtt gcaacagcaa accttggtac ttctccatcg aaatacttgt taaccatagc
     1981 aatagtgcgg ttgagaaggt ttcctaaatc attagcaagt tcatagttaa tacgtccaac
     2041 ataatcttct ggcgtaaaag ttccgtctga accaacaggc aatgaacgca tgagataata
     2101 acggagtgga tcaagaccaa aacgctctac taacatttct ggataaacga cattcccttt
     2161 tgatttagac attttaccat cttgcatcac aaaccaacca tgagcaacta gacgttttgg
     2221 taatggcaaa tccagcgcca tcaacatgat aggccaataa atagagtgga aacgaagaat
     2281 atctttacca atcatatgaa tgtctgctgg ccaaaattta tgatattgac tgagatcgtc
     2341 agaccaacca taaccaagag cagaaatata gttcatcaaa gcatcaatcc agacataaat
     2401 aacatgtttt ggatttgatg gtacctgaac tccccatgtg taagttgtac gagacactgc
     2461 taaatcttct aaacctggtt ctataaagtt tttaagcatt tcattcatac gaccatctgg
     2521 ctgaataaat tctggatgct cagcgtagta cgcttttaaa cgatctgcat atttgctcat
     2581 gcggaagaaa taagattctt cagaaacttt ttcaacttca tgaccagatg gcgcaacacc
     2641 accaatcata ttaccgtttt cgtcacgata aacttctgct aattggcttt cagtaaaaaa
     2701 ttcttcatct gaaacagaat accatccggt atactcacca agatagatgt ccccttgtgc
     2761 caacaattgt tcaaaaattt tagcaaccgc ttcttcatgg taagtatctg ttgtacggat
     2821 gaatttgtca taggaaatat ctaaaagttc ccataatgtt ttgacacttt cagccatgcc
     2881 atcaacatat tcctgtggtg taatgcctgc ttcttcagcc ttttgttgaa ttttctgacc
     2941 atgctcatca agaccagtca aatactgaac atcaaagccc atcatgcgtt tgtaacgtgc
     3001 caaaacatca catgctatcg ttgtataggc agaaccgatg tgcaatttac ctgatgggta
     3061 ataaattggg gtagtaatgt aaaatgattt tttttctgtc atgatttctc ctataagcca
     3121 ccctaatgag aggtgttttt tatcatgata cattataaca taaaatcttt ttcaactgga
     3181 tatatctctg aataatatat ctacttttgc tataataaag gtattaaaaa tagagagtta
     3241 atatatggtt aatacaaaaa cacttctccc ctacaaaact atgccggttt ggactgcaca
     3301 gtctattcca aaggcatttt tagaaaagca taatactaag gaaggcacct gggcaaaact
     3361 aaccattcta agtggttctt tagtatttta ccagttatct cctgatggag aggaaatctc
     3421 gcggcatatt tttgatgcta gtagtgatat tccttttgtt gatccacaag tctggcataa
     3481 agtttcgccg aatagtccag acttaagttg ctatctaact ttttactgcc aaaaagaaga
     3541 ttacttccat aaaaaatatg gtctcacgcg cacacattct gaggttatcg ccagtgcacc
     3601 tctcttatct gagaagagta atatattaga ccttgggtgt ggtcaagggc gaaactcact
     3661 ttatttatcg ctgctgggac atcaagtgac ttctgtcgat tcaaacggac agagccttgt
     3721 agctttagaa aatatggcat tagaagaaga gcttccttac aatataaaaa ggtatgatat
     3781 taatactgct gctattgaag ggcactatga ttttatttta tcaactgtgg tatttatgtt
     3841 tttaaatccc gactgcattt ctgatattat tttacaaatg caatcacata ctcagattgg
     3901 aggatacaat cttatcgttt ctgctatgga taccgctgaa aatccttgcc cgcttccatt
     3961 cccttttacc ttcaaagaag gacaattgaa atcttattac aatgactggg aaatcatcaa
     4021 atataatgaa aatttaggtg aattgcacag ggtggatgaa aacggtaatc gcttaaaatt
     4081 acagtttgcg actttattag ctcgaaaatt aagatcataa aagtgagtag aaatctactc
     4141 acttttatct ttggttaggt aataagtata ttctaaaccc atacataatt atcctataaa
     4201 atatatagag atgatatttc aaatcattat tgtaacaatc agctttagta tagctcttca
     4261 acataaaacg ccatacatta ggagtgcctt tctacttgta ggttataaaa ttcatggcct
     4321 aacagcaagt tgacatataa aagaggaatt aatattaatg ataagctcca ttcgcccagt
     4381 ttacttgaaa aataagtcga gcaaacaaca ccaaagataa atcctccaat aataataatc
     4441 gtatttctag caattaagaa aatatcagaa tttttttcgt aaagtccctt tgttaataaa
     4501 taagcagcat tcttgatatt accagtcatc atcatattag cataaggtgc tcctctgagc
     4561 gatttaaagg tatctacttg tattgaagca aagaaggcta gtcctgccac agtaaacgac
     4621 gatggtaaga aaggagtcaa tataattgtt actatagcaa cctgtgtcaa cgcaaaacta
     4681 gataataggt accagtgcag ttgatgttta ttcgcccaac gattcatgaa ataagtaaac
     4741 gattgaccta acatgaaaac cattataggt agcaaaaaag caagcgcttg actataatgc
     4801 ttattactaa gatgaatagc aaaagataac aaattccctg tttgtacacc tgcaaaacgt
     4861 tttccttgtg ttacataagt aaaagcattg atataacctg aaataaaaca gagtgcaacc
     4921 gcacacctga gcccttcata tatttgatat tgacggtgag actttttaga catattttcc
     4981 tctaacttta tggcaaacaa aaataagact gggcaatctt gctcagcact ttttttctaa
     5041 tagattggtt taaaatagta ttaatattag cactagctag cctaacagtt ctaaataaat
     5101 tattaagcaa tgataaatgt agttttagta tatcataacc tcagcattga agcaatctca
     5161 ctttatgaaa ggataaagaa aatctcataa gtcatttacc cttaatttga agtttaatta
     5221 gttactttta attttactac caaaaaactt gcaccattct taacatttgt tacaataaat
     5281 acaacctatt aggaggtcag tacagattat gcacagcact atttataaat gtcatcattg
     5341 ccgttatcta atttataaaa gttttaaaga tatctttcct tttgctctca ttttttcttg
     5401 gattgaaatc cttcaaaaac tcgttatatc agatgattct tttttagctc aaacaacaaa
     5461 gtccagtatt tataaaaacg agtggttata tacggtttcc aatactcttt atgatttatc
     5521 tcatttacta atctacttta tggctagtta ttttgtggct agtttagtat ggagagactt
     5581 atctaaccaa actaaagaaa gtttttctgc aagtttactt ggctttgcca ctgtttggtt
     5641 gcttctaact tcagttgttc caggtacaga ttatcactat cctagccaac ccatttggct
     5701 tatgctggct ttgatttcgt atctttttat ctttttaact cgactgcttc aaaatcgaca
     5761 tcatattttc cagtatagta tgttagctat actactatta atctcttcta ttttgtcaca
     5821 tatcttatat cgttatccta atattgattt agaatacctt atacaaaatc acttttctca
     5881 atggctgggt gatgggccac atcaattaat tcctattatg ctttggtcta tcttagctat
     5941 ttttctaacc ttatcaggtc tttttgtacc agatatttta tttcgtcctt actcctattt
     6001 ttcagtagtt agcgaaaatt taaatgctgc cttgagccaa catacagata aaatccctta
     6061 tctgtatacc ttttatactg ttaaaaatag ctttgctatg tttggcggta ttggaatcct
     6121 attatcctta tttttagcag ttttatatga aagtagaaaa ctacaatcga agaattacta
     6181 taaattgaca ttactgacct taactcctct tatttttgat caaaatttac catttttagt
     6241 aggtctacct gtcattttac aaccaatatt attcattccg atggtattaa caactatctt
     6301 tgcagaggct tttggagctc ttatgctcta cctaaaattt gtcgaccctg cagtttatac
     6361 tgtcccaagt ggtacaccta gtctactatt tggctttctt gcttcaaatg gggattggcg
     6421 ttaccttcca gtcactgcaa ttattctagt tgttggcttc tttatttatc gtccgtttgt
     6481 caaaatcgct ttcgctaagg aggagcaata tgaaaaaatt acttagatgg cttcctcctg
     6541 tacttttcat tattatcctt ataggaatga ctatcttagg taagtcctat atcaataaag
     6601 taacagctca caaaataaaa ctctataact ctcgaatgac tcctactatt ttaattccag
     6661 gatccagtgc tactcaagaa cgatttaaca gcatgttagc acagctcaac caaatgggag
     6721 aaaaacatag cgttttaaag ttaactgtca aaaaagacaa tagcattatc tacaatggac
     6781 aaattagcgg caatgaccac aaaccctaca ttgtcattgg atttgaaaat aatgaagatg
     6841 gttatagtaa catcaaaaaa caaacaaaat ggctacagat tgctatgaat gatcttcaga
     6901 agaaatataa atttaaacgt tttaacgcta tcggtcattc aaatggtggc ttatcatgga
     6961 ctattttcct agaagattat tacgactctg atgaatttga tatgaaatca ttgttaacaa
     7021 tgggaacacc ttttaacttt gaagaaagta acacctcaaa tcatactcaa atgcttaaag
     7081 atttaatcag taataaagga aatattccat caagtctcat ggtatacaat ttggcaggaa
     7141 ctaattcata tgatggtgat aaaattgttc catttgctag tgtggagact ggtaaatata
     7201 ttttccaaga aaccgctaaa cactataccc aactaacagt aactggtaat aatgctacac
     7261 attctgactt gcctgataat cctgaagtta tccaatatgt cgcagaaaaa attcttaaaa
     7321 atgagaaagg taaattacca aaacctcact aaagctgtaa aatagcaacg cctctggtat
     7381 tttgatataa tatattgcga attaaactaa ataaaaaagg atattattat gaaactcatt
     7441 tcatggaata ttgattccct caatgcggca ttaacaagtg aatcaacacg tgctttaatg
     7501 tcacgccaag ttattgatac tttagttgct gaagatgccg acatcattgc gattcaagaa
     7561 actaaactgt ctgccaaagg acctactaaa aaacatttgg aagtacttga aacttacttc
     7621 ccagaatatg atttggtttg gcgatcatca gtcgaacctg ctcgtaaggg ctatgctggt
     7681 acaatgttct tatatcgtaa agggttaaat cctattgtta gcttccctga aatcgatgct
     7741 cctactacaa tggataatga aggtcgcatt attactctcg aattggaaaa ctgctatatc
     7801 acacaggtct atactccaaa tgcaggtgat ggcctaaagc gcttagctga ccgtcaaata
     7861 tgggatatta aatatgctga gtatttagca acattggata gtcaaaaacc tgttctagca
     7921 acaggtgact ataacgtagc ccacaaggaa atagacctag ctaatcctag cagtaatcgt
     7981 cgttctgctg gattcacagc tgaagagcgc caaggcttta caaatctact agccaaaggt
     8041 tttacagata cttttcgtta ccttcatggc gatgttccaa atgtttatag ctggtgggca
     8101 caacgaagtc gtacatccaa aatcaacaat acagggtgga gaattgatta ttggctaaca
     8161 agtaaccgtg tcgcagataa aattaccaag tcagaaatga ttcattctgg tgatcgtcaa
     8221 gatcatacac caattatttt ggagattgaa ctataaacca aaaaaagatg attgagttat
     8281 aactcaatca tctttttata ctaagttgag gtctttatac ttagtacggt agccaatctg
     8341 aagaatttta ttatctttaa ctagcaatgg acgcttaata agcattccat ccgatgccaa
     8401 taagtccaaa gcttggtcaa gagttaaatc atcaaattta tcttttaagc ctaattctct
     8461 gtaagaattt cctgaagtat tgaaaaactt tttaaggtca taaggcgaat tctctaacaa
     8521 ttcttttaat aacgaaactt tgggaggatt gctttttata tcaatcgctt caaaagtcaa
     8581 gccgagttca gttaactctt tttttgctga acgacaagtg gtacatttgg ggtattcata
     8641 aaacgtatac atattatttc tccttttcgt attctaataa ccaagatttt ctttctaagc
     8701 caccagcata accagttaat tgaccatatc ttcctaaaac acggtgacaa ggtataatta
     8761 gagaaatagg atttttacct attgctcctc caacagcctg tgcagattga caatttagct
     8821 ctttcgctaa ttctccataa gttttggtat ctccaaaagg gattttctgt aattcctgcc
     8881 atactctcgt ctgaaatgct gtccctctca atgataatgg aatactaggt aaattttgct
     8941 gtgaaaaata agcatctaac cacttcttac caagtaaagt ataggaattt gacatattaa
     9001 caatttcctc ttggttgact gctagcatat catatttttg cccaacaaaa taaagccctg
     9061 ataaacctag attgtctgct agaaggcgaa tttctccaag aggggactga tagaattctt
     9121 gatataacat atgataagca taacttaaaa agccatgaaa agcaattcat gactttttag
     9181 ttctttattg ttgttttgca attaaacgta cacggacgat attctcatca ccttcaaatt
     9241 cttcaattaa agtagaaatc ttgttattat ccgtttcatc taaatctatt agtgtgtagg
     9301 cgtagtcacc ctttgaacgg ttaataatgt tatcaatatt tatacctaac tctgatacag
     9361 cagttgaaat tttagccaca atgttaggaa cattcttgtt aattaaagta atacgaaatg
     9421 gtgcggtttg aatttgatgt acattgggga aattaactga attagtgatt tctcctgtct
     9481 ccataaaaca acgaatggtt tgacttgcca taatagcaca atttagctct gcctcatctg
     9541 ttgatccgcc tacatgagga aagactgtaa ttcctttttg gtttaataat tctttgtctc
     9601 caaaatcagt aatatagcgc ttgacaacac cagtttctat cgcttcaaat agctcttggt
     9661 tattgactaa ttctgcacga gcaaagttga taatcgtagt tcttttttca tgattgaaaa
     9721 agctttcgca tcaaaagtat gcttagtttc atttgttaag ggaacatgaa ttgtgatata
     9781 gtcacaagtt tcaaaaatat ccttaatctc tttaaccctt tgaacatggc ttgaaatatt
     9841 ccaagctgtt tcaattgaaa catagggatc ataaccaaga actgtcattc ctaagcgtct
     9901 agcatcattc gcaattctag ctccaatggc accaaggccg ataactccca atttttttcc
     9961 tgcaatttca ttaccagcaa aagctttctt tcctgcttca atttgtttgg gaatatctgt
    10021 tccagttaga gtattaaccc atcggttagc tcctaaataa tcacgagctg aaagtaataa
    10081 ggcagcaatg accgcttctt ttacagcatt agcatttgca cctggggtat taaacacgac
    10141 tattccctgt gcacttgcct cttcaatagg aatattattt gttcctgcac ccgccctagc
    10201 aatagctttg aggttacttg ggaaatcctg attatgcaaa ttttgactac gaataatata
    10261 ggcatcagga ttttctgaca tatccccatc aatctggaaa cgattcccta actcttgtaa
    10321 accaatttga ttaatattat taaatgtttt gacactaaaa accattcttt ttcactaagg
    10381 ttatctcttt agtcatcatt gaccaaaata acccctttct ttttttctta catgggattt
    10441 ctttcatcaa tctcacgata gatgctcgtt tctccctttt cttttatctt ctgataagcc
    10501 aatcgtaccc catctagagg tactttacca caatattgat agcctaattt atttagaata
    10561 tgctgcatag tgacattttt ttcatgagta tcgcaacgaa aatcaggtcc tttatgacct
    10621 tcaattaacc cttgaaggaa tgtctgtgct agtcctctac cacgaaattg atttgagatc
    10681 gcaatacgat gaaatgtaag atagcgatgg ttatcatgga gccattttcc ctcataaata
    10741 gcatcataaa cctcttcatg accatcaatg acagccgcat aagtcgctag catcccatct
    10801 tcaataccaa cccaagcata accgttcaag atatcatcaa taatatcgtt acgattaggg
    10861 tatccatctt ctttttgcca ttgatcactt cctgttttag caatttcggc acgcgcttcc
    10921 tcaataagaa gcataatttg gtcaatttca tttggaaaag ctaatctgat ttgcattata
    10981 ttacccactt ttatatatta tagactataa taacggttta attttctttc gtgataacat
    11041 ttactatgta ttttctgctt caaatgtttt cataaaatca attaattcga tgacaccttg
    11101 tttaggaaag gcattataaa ggctagctct cataccacct accgaacgat gtcctttaat
    11161 atttttaaat ccacgttctg tcgcttcctt tacaaatttt taatctaaat ctttattaat
    11221 agttatgaaa ggaatgttac atagagaacg actcttttta tctctgacag gattactata
    11281 aaattctgag gaatcaatat aatcatagag aagtcctgat ttttcacgat tagctttttc
    11341 catagcatca acaccaccta gacttttcac ccattcaaag actaatttag caatgtaaat
    11401 actgtaagca ggcggcgtat tataaagaga accagcatct gactgaatct tataatccaa
    11461 cattgatgat aaagttggtt cctcattaat catatcttca cggataataa ctaccgttac
    11521 accagcaggt ccaatatttt tttgagctcc tgcataaatc attgcaaaat cttctacttt
    11581 atattttaca gctaatatat tagaagacat atccgcaata acaggcactt cattagtttt
    11641 aggaatatca taaagcgatg ttccttcaat ggtattattt gttgtcacat gaacataggc
    11701 agctttagga tcaatctctt tttcatcaaa ctctggaata taatcataaa ctgattcttc
    11761 tgatgaagct aatagaattg gttcaaaagg aattgttttt gatagtttta cagcctctgt
    11821 ataagctttt ttccccccac gaaccagcaa catgatagta agctttacgc cctctggcaa
    11881 tattcaaggg aatcatacta aactgtaagg atgcaccacc ttgtaaaaag attactttat
    11941 aattatccgg aattcccatc aaatctctta agtaacgctc agcacctttg ataatatcat
    12001 caaactcttt ggagcggtgc gatacctcca aaacactcat actagaccct tgataattaa
    12061 ggagttcaga ttgggctttt acaagcactg gttttggtaa gactgcagga cctgcagaaa
    12121 aattataaat tgtcatataa cacctccata taattgtgta agattatagc acagtatttt
    12181 gaatattgta aagatttttt tgattaattt tgctttttat tcgtgatata atggtaaagg
    12241 cgaacaatca agtttaaagg agtaatttat gattttgagg gaattttgtg cagagaattt
    12301 aacagactta accagattag ataaagctat tatttcacgt gtcgagcttt gtgataacct
    12361 tgctgtcgga ggcactactc ctagctacgg tgttatcaaa gaagctaatc aatatttaca
    12421 tgaaaaaggt attagtgtcg ctgttatgat tcgtccccgt ggaggaaatt ttgtatacaa
    12481 tgatttagaa ttacgtatta tggaagagga tatcctacgt gctgttgagt tagaatcaga
    12541 tgctcttgtt ttgggaatat taacttcaaa taatcatatt gatactgaag ccattgaaca
    12601 acttctacct gctacccaag gtttgccact tgtttttcat atggcatttg atgttatccc
    12661 gaaatctgac caaaaaaaat caatagatca attagttgct ttaggcttta caaggattct
    12721 cctacatggt tcatctaatg gcgaaccaat tatagaaaat atcaaacata tcaaagcatt
    12781 agttgaatat gctaataatc gtattgaaat tatggttggc ggcggtgtga ctgctgaaaa
    12841 ctatcaatat atttgccaag aaactggggt taaacaagcc catggtacta gaataactca
    12901 ataaaaaatt cgaaacaggg ctataagttt ctttagtata tttactacta agttctctca
    12961 tgttttctaa aacctgaaag aaccggaata agagcatacc atcttttata acctactctc
    13021 agaaataaaa tccaatttgc aatggtattc aaaagagctg aaattaaaaa ttttaaggaa
    13081 attggaagta tcttacttct ttattccaat agctaaatcg gagattataa aaggttgcca
    13141 aatttttctt tggcaacctt tttatattat tacttataaa tcatggaaac tagcatagag
    13201 ttcttgtcta ttgagattaa attcttttgc tacttttttt atcgcttgat tagttttatc
    13261 accattagcg atatattctt ttactaatac tagtggatct tgttggctac tgtctttcac
    13321 tcgctcggta tctctcttac catcaacaat aattaagcat tcacctttga gagggacctt
    13381 ttcaatatgc tctaaaagtt gactaatggt tcctctttga tactcttcat agagtttcgt
    13441 caattcgcgt actaaaacaa cttggcgatc tccgtaaatc tctttcatgt gttttagcgt
    13501 atcagagact cgaaacggtg actcataaaa gatttgtgtt tcagggtaat cttgctttgt
    13561 ttcaaagaaa gttatttgtt gacctttctt acgtggtaag aagccataaa aaatatgagg
    13621 ttgtggagct aaacctgaag cgatgagagc agtaatacca gcgctagctc ctggtataga
    13681 tacaactggg atatcccctt caatagcagc cttgacaagg tcatgtcctg ggtcagaaat
    13741 agagggcatt cctgcatcag atacttgggc taaagatttc ccttctttta acaaatcaat
    13801 taacccagag attttatcgt aagcattgtg ttcgtgaaaa ctaatttgtt tagtagtaat
    13861 atcaaagtgc ttgagtaaaa gtcccgtatt tcgtgtatcc tctgcacaaa taaaatcaac
    13921 ttctcttaaa atcctaatgg cacgaaaagt catatcatct agattaccaa ttggagttgg
    13981 gactagatag agtgttccgt aatgtatatt tgatttaaaa cttttttgaa cttgcatttc
    14041 catcaatctc tatataataa ttcaatacaa aaagcacatt cttcgtcatt ttcacgacgt
    14101 tgaccataga aagtattaca aatgtgaaag ccatcattgt aaatgccttc gagtaattct
    14161 cttccttgtt tagaattttt aactgcagtt tctgtttctg cttcaataac gctcagacgc
    14221 tcacgaagtt taccattttc aatgcgtaga acagtattct cctccaataa tttttggatt
    14281 tgttttttca tagtctcaat ttcagacaac ccaactaata aattttgtga aaaatcatca
    14341 aaggcatcaa acaagtcttt tttatccatt cactcttcct ctctgcgcgt attattttct
    14401 aagaaagcac catatactct aaactatttt gaaaactaac attgctcttc cacatttgct
    14461 gagctctata agtattttct aaatcataaa gttggccttc tttagccagc attattgtca
    14521 gccattgaaa aacaaatgcc tgatcatttt tatctacaac ttgcgatgtc aagcgagata
    14581 cttctaaata agcagtttgc ctatcttttg ccaaagtcgt cacaaactgt tgacttaaat
    14641 tcaagtaatc taataacttt ttgttagtca acaatatttc taaatgttta ggatcgtctg
    14701 ctacttgagc taataagcta gcttgtgatt ttagcaatcc tgcttgttca gcttgatgaa
    14761 ccaacatgtc caattgtttt ggaaaacgaa aaatttgcgt tctactctta attgttggca
    14821 aaacattgtt ttcatcatta gtcaataaga tgacataaga tgaactttga ggttcttcaa
    14881 taaatttcaa aagactgttg gctgcattga catgcatttt ttcgcaatct ttgatgataa
    14941 agacctgtga tttcccttcg aaacctgatc ttgaaaaatc tttagtcagc tcttttatag
    15001 tctctgtctt aataagttgc ccctgtggtt ctatgatttt aacatctgaa aattccccat
    15061 tagcgattaa acgacaggct ctacactctt gacatggtaa accactctgg cgcttttcac
    15121 agaactgact ttgtgctaaa taaagtgcca tatctagact tgcaaaattt cccgaaaaaa
    15181 gataggcatg actcatccta tctgactgta aaatagtatt aaacttttcc agcaatttgg
    15241 gttgtgttcg ctttaaatcc atgacctttg tacttcctga ttcattggtt gatacgatct
    15301 aaaataatag agaatgtctc agctattact tcatctagct gttgggaggc atcaatcgtc
    15361 acaatgcggt ttggctccga atccgccaat tcaaggtaac cttgacggac gcgttgatgc
    15421 atatctaatt gctcgagatc tagacgattt acctcccttt gaacactttt ttgaatacgc
    15481 tctaaaccta cttcagaagg aacatcaaaa tataaagtta aatcaggttt atgactatca
    15541 gtcgcatagt cattaagcca ttttatatgt gacttatcta atcctcttcc tgatccctga
    15601 taggcaacag aactatcaat aaaacgatcc attaggacta ttttcccttc ttctagagct
    15661 gggagtacct tttccactag atgttggcgt cgagcagcca tgtaaagcaa tagctctgtt
    15721 tttttatcca tttgcgtgtg tttaacatct aaaataatat gacgaatttc ttctgaaata
    15781 gttacgccac caggctctct ggtggtgaga atatcttgtg ataatttttc tctgagcaag
    15841 ggtaaaacag cttctaaaac ggttgtttta cccgcaccat ctgggccctc aaaagaaatc
    15901 attagtcctt ttttcatggt acatccttta tttataatta ctactattct atcaaaattt
    15961 atcaaaaaaa gacacccaca acttgattgt gcatgtctga tatttttaag agtaatttct
    16021 caaagtaagt tccgtctgcc ctccaaaacc ggaagttagt gtgaaaagga tttttatggt
    16081 ggaattaagt ctgagacgtt tgggttagaa atgagtttat ccatcatgac aaaacacaaa
    16141 cacctaactc tcttagaccg taatgacatc caatctggtt tggatagagg agaaaccttt
    16201 aaagccatcg ggcttaatct actgaaacac cctactacta tcgcaaaaga agtcaaacga
    16261 aataagcaac taagagaatc aaccaaagac tgcctagact gtccgctact aagaaaagct
    16321 ccctatgttt gtaat
//
LOCUS       DATRMA010000042        14539 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.042, whole genome shotgun sequence.
ACCESSION   DATRMA010000042 DATRMA010000000
VERSION     DATRMA010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14539
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.042"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            47..1039
                     /locus_tag="VBC28_001283"
     CDS             47..1039
                     /locus_tag="VBC28_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO6815266.1"
                     /translation="MSMIEVSHLQKNFIKTVKAPGLKGAFQSFLRPEKHTFEAVKDLT
                     FDVPKGQILGFIGANGAGKSTTIKMLTGILKPTSGFCRIDGKLPQENRQNYVKDIGVV
                     FGQRTQLWWDLALQETYTVLKEIYDVPDKEFRKRMAFLNEVLELNDFIKDPVRTLSLG
                     QRMRADIAASLLHNPKVLFLDEPTIGLDVSVKDNIRRAITQINQEEETTILLTTHDLS
                     DIEQLCHRIFMIDRGQEIFDGTVSQLKETFGKMKTLSFDLRPGQEHISSSLIGKSEIN
                     IKRNDLVLDIQYDSSRYQTADIIQQTLADFSVRDLKMTDADIEDIIRRFYRNEL"
     gene            1042..1860
                     /locus_tag="VBC28_001284"
     CDS             1042..1860
                     /locus_tag="VBC28_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HEO6815267.1"
                     /translation="MNRIWRRYKPFISTGIQGLITYRVDFILYRIGDVIGAFVAFYLW
                     KAVFDSSSQSLIQGFQLSDMILYIIMSFVTNLLTRTDSSFMIGDEVKDGSIIMRLLRP
                     VHFAASYLFMEIGSRWLIFLSIGVPFLLVITGVRLFLGTDLIQAIVLVVFYIISIILA
                     FLINFFFNICFGFSAFVFKNLWGSNLLKNSLVAFMSGSLIPLTFFPKIVADILGFLPF
                     SSLIYTPVMIIIGKYDGSQIVQALLLQIFWLIVMVALSQLIWKKVQLHITIQGG"
     gene            1862..2647
                     /locus_tag="VBC28_001285"
     CDS             1862..2647
                     /locus_tag="VBC28_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6815268.1"
                     /translation="MTKYQRMHFIFIKQYMKQIMEYKIDFFVGVLGVFLTQGLNLLFL
                     NVLFQHIPSLEGWTFQQIAFIYGFSLLPKGIDHLFFDNLWALGQRLIRKGEFDEYLTR
                     PISPLFHVLVETFQVDALGELLVGFILLSTTVSSISWTVPKVLLFIFIIPFATLIYTS
                     LKIATSSIAFWTKQSGAVIYIFYMFNDFAKYPVAIYNNLLRWIISFVIPFAFTAYYPA
                     AYFLQDRNVYFNIGGVILISLISFMVSLILWHKGVEVYESAGS"
     gene            3272..3577
                     /locus_tag="VBC28_001286"
     CDS             3272..3577
                     /locus_tag="VBC28_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cylX protein"
                     /protein_id="HEO6815269.1"
                     /translation="MGRSNILSRCEQCSDNQCGLYKTRLKDKLLLVVQLVGNIRYSEF
                     LTEGGVYHKGETLLEVITLGISYEYKSYGDIKVIKKYCADGSVVDYNMPILLMERIE"
     gene            3577..4425
                     /locus_tag="VBC28_001287"
     CDS             3577..4425
                     /locus_tag="VBC28_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CylD"
                     /protein_id="HEO6815270.1"
                     /translation="MKTVIIYGGQGNVQSKHLCQLVKTPKGEILFSKLQATDLESYQL
                     MMDVINHKVSLDNVHASMLANFLYNDWLTNDEKIVSGIGFSAHSAGIFNVLYASGSTS
                     FENIIHFIKRRAQLIENCHVSEELWLLMVENIDMFSKDVLKEQSEKVKLAIITDDRAG
                     VIAMDQVNRSFFDDVMTNKGYFYKLKQLGVKAPYHTDFLNESKEQYKQLIESLDISQN
                     DDYDYIFHCDQLVDEILYQWTNLFNWRAIKETIIEKDAEIIDISPNRFISSQLQKMKK
                     RGRKQL"
     gene            4422..5144
                     /gene="fabG"
                     /locus_tag="VBC28_001288"
     CDS             4422..5144
                     /gene="fabG"
                     /locus_tag="VBC28_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HEO6815271.1"
                     /translation="MKVAIVTGGTRGIGRAITKELYKEGYKVIAIYNSNDAKARALQE
                     ELPKLDVYKCNISDAKAVQKLVTKIFREYGGIDCLVNNAGIVRDGFFLMMSKEKWMDV
                     ININIMGLVNMSKAVLKIMKAKRIQGKVINISSTSGIAGQIGQANYSATKGAIISITK
                     TLAKEFASDGITINCVSPGFIETDMTNELQNKEELKEHLIPLKRFGQPEEVAWLVSFL
                     ASEKANYITGKNIVIDGGMIND"
     gene            5137..5442
                     /locus_tag="VBC28_001289"
     CDS             5137..5442
                     /locus_tag="VBC28_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEO6815272.1"
                     /translation="MINWAEERKIIKTRQEVVDEIKGILIDSLMLDIDKDLIMNDQPL
                     FGRGLELDSIDALELSIGISTTFGVELNDDDISILSSVNRLADFVIENSEDIDDQAK"
     gene            5426..5902
                     /gene="fabZ"
                     /locus_tag="VBC28_001290"
     CDS             5426..5902
                     /gene="fabZ"
                     /locus_tag="VBC28_001290"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEO6815273.1"
                     /translation="MTKLSSEEIIKMLPHRYPFLMVDRVQSYKKGIIECRKNITINEP
                     YFQGHFPQRPIVPGVLMIEMAAQSAALLYILDALDGSLPSLDELTAEKIAEKVGYLAS
                     VKNFKFKKIVTPGDQLNITCKSQSKLGHLLEIQVVIRDENKKEVASGRMLVSENGN"
     gene            5892..6821
                     /locus_tag="VBC28_001291"
     CDS             5892..6821
                     /locus_tag="VBC28_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6815274.1"
                     /translation="MEIKLKNIGKKYGSFEALRDINLIFEEGKFYGLLGPNGAGKTTL
                     FNLLIQNFKQTSGDIKWEVDGKPLSIKDFYRHIGIVFQSNRLDDNLTVEENLISRGAL
                     YGLSKSQVRNRLKDLQTYLDITAIKKQKYGSLSGGQKRKVDIARALLPQPSLLLLDEP
                     TTGLDPQSRRDLWDAIAQLNQQSQMTVVLITHYLEEMSACDVLNVLIEGNIYYSGDIK
                     SFIEQHSTTNLNVVLKPEKSLDQLSIADFVNKCQVLSEREIVFKDISVEEMMQVISDN
                     QDKSIIETFNVEYSNLEAAYLNLLREKERKNNV"
     gene            6814..7692
                     /locus_tag="VBC28_001292"
     CDS             6814..7692
                     /locus_tag="VBC28_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6815275.1"
                     /translation="MFELISRNRKVYTRDRLAFFMSFLSVIILILVYQIFLGKIQLDA
                     IKAALRSNTVSSDTIHMVNYWLIAGLTTIISMTSTLGAFGVMVSDKEKKLNEDFKVSP
                     ISNIKIELSYAIFAVLFGIVMTMFSCIFAIGIFNGFNSLLEYSAMDYLTILGIVSLGT
                     VLSAAMILPILAFIHTSSAFTTLSTIVGTFIGFISGVYLSIGSVGKALQQVMTWFPLT
                     QINSLLKQVLMKGSIAKVFDKANEATVSNYKESYGVVLRNADGERLSNHFMLIYIIAL
                     ILILLAIHFIIKKVKK"
     gene            7689..9692
                     /locus_tag="VBC28_001293"
     CDS             7689..9692
                     /locus_tag="VBC28_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cylE protein"
                     /protein_id="HEO6815276.1"
                     /translation="MKDDNKLKISEASLEDYSEVVHLFNRNHVYQFPDGRPLTVDDLD
                     LTLKVKEVTHLFLLKNHGVLIGTSAFFKFITYGCLDWNSSFSGFLLIDSKSRSGQAIT
                     YLYKTILKKITKLKFSNIYTEISNYNKPSLALSKLNGFKEYDKTYEDILHCRSLRSHL
                     PKILNTFRISNYYGKTYDISTFQIMEEIENPLEEETEIRTKVSDEEILFKAEDSASLP
                     YYLKMSLFQMEIAKLDNRYVLQVDFLSEQVKRVRVKTGKYHLANLTRAHPSLTLSRFA
                     NYYYIQATVETLYGNIDVQLERRKKHYRDATICLKRTFQGYDLLISPNGSLIFEKQKR
                     KILEDSFLIFSQPLDKKLVVKEEENHITIKCFYQGALIEKIMTFTSDEEITCVYKCNQ
                     KAKEMFPKLLKQTFKLHCQEQLIRDSEGYLVNVPGSYPIEHDDFLRADKFEDRQFHYY
                     LPNEDKMISYSPPGKASNQMQFRPLCLIDTDSLSFPLTYHFRISQASSEEALINLKKQ
                     PIWDSNYQASATDLLKHISNLTLEEEKDYGIKRMIANRKHYPSHKLVLAYNQIVLPKN
                     EIPRDSELYSISFDYRIRGKFVQIRQGEHVKYDNKSYVLENSQQLVLYVASDDKYILI
                     SAKNGIFYSYKENNHLKIRCMFKRNSPYATNVSITEYRKCEEK"
     gene            9689..10642
                     /locus_tag="VBC28_001294"
     CDS             9689..10642
                     /locus_tag="VBC28_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyltransferase family protein"
                     /protein_id="HEO6815277.1"
                     /translation="MNTIYNTLRTDKGYKVYEGYLYEITGEECEEALDLVIPKNIVFA
                     DTDTCGYTFLLNEDGTVYDDVTFYKFDDKYWLASHKALDSYLDNINFDYTVTDISDEY
                     KMLQIEGRYSGEIAQSFYEYDISTLNFRTLIEMTYKGEKGYLARFGFSGEFGYQFFLP
                     SSIFATFVSDVCEGIAEYGDELDRYLRFEVGQPITDIYQQEEYSLYEIGYSWNLDFTK
                     EEFRGRDSLLEHIRSATVKSVGFSTKEKLASGTPVLFDDQIVGKIFWIADEKDSSENY
                     LGLMIVNQTYAHSGVTFVTEDGQILKTQSSPYCIPESWNKE"
     gene            10639..12834
                     /locus_tag="VBC28_001295"
     CDS             10639..12834
                     /locus_tag="VBC28_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000118219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HEO6815278.1"
                     /translation="MSVYVSGIGIISSLGKNYSEHKQHLFDLKEGISKHLYKNHDSIL
                     ESYTGSITSDPEVPEQYKDETRNFKFAFTAFEEALASSGVNLKAYHNIAVCLGTSLGG
                     KSAGQNALYQFEEGERQVDASLLEKASVYHIADELMAYHDIVGASYVISTACSASNNA
                     VILGTQLLQDGDCDLAICGGCDELSDISLAGFTSLGAINTEMACQPYSSGKGINLGEG
                     AGFVVLVKDQSLAKYGKIIGGLITSDGYHITAPKPTGEGAAQIAKQLVTQAGIDYSEI
                     DYINGHGTGTQANDKMEKNMYGKFFPTTTLISSTKGQTGHTLGAAGIIELINCLAAIE
                     EQTVPATKNEIGIEGFPENFVYHQKREYPIRNALNFSFAFGGNNSGVLLSSLDSPLET
                     LPARENLKMAILSSVASISKNESLSITYEKVASNFNDFEALRFKGARPPKTVNPAQFR
                     KMDDFSKMVAVTTAQALIESNINLKKQDTSKVGIVFTTLSGPVEVVEGIEKQITTEGY
                     AHVSASRFPFTVMNAAAGMLSIIFKITGPLSVISTNSGALDGIQYAKEMMRNDNLDYV
                     ILVSANQWTDMSFMWWQQLNYDSQMFVGSDYCSAQVLSRQALDNSPIILGSKQLKYSH
                     KTFTDVMTIFDAALQNLLSDLGLTIKDIKGFVWNERKKAVSSDYDFLANLSEYYNMPN
                     LASGQFGFSSNGAGEELDYTVNESIEKGYYLVLSYSIFGGISFAIIEKR"
     gene            12839..14050
                     /locus_tag="VBC28_001296"
     CDS             12839..14050
                     /locus_tag="VBC28_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cylJ protein"
                     /protein_id="HEO6815279.1"
                     /translation="MSKFLFAPFMMNLGESQRLSKLANCLYEQGHDIHILGDNYYPFL
                     FNNDAYRYHHCVEDKSVYNSKRYDAFFSLSTDFNFLSEEEIERICSIERDLLRQEKFD
                     AVLTGYRLSIVTSCRLESIPLIWIISGATHISEIVENSEGILPNGKISKASKPQTKDF
                     IKRVIITYSTNVKTWNNYIKKYGGKPFNNALELFTGDLNLVTDYSLFYEFDKDSSYKT
                     IGPILIDNVGFSKCSQINQDNKTVLLSFGTSFKRDWVESFLKTLPRHYHYLLTSCGEA
                     INIPGSYIEIVDFIDFKTIDKEIYFAIIHGGQGTVYAMAAQGIPFIGIPFFNEQFWNI
                     KKFSKEKCAYLVKKPDVREIRKAISCLERDYDIYKSYMIRLSQGIKEEAEASLQKAEA
                     EIESFIRKRQS"
     gene            14019..>14539
                     /locus_tag="VBC28_001297"
     CDS             14019..>14539
                     /locus_tag="VBC28_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CylK protein"
                     /protein_id="HEO6815280.1"
                     /translation="MNPLSEKDKVNYIMIYKNRQSTLENNLSGDRLMRYQQANRQVSK
                     DKLLTGSYFVYQCLKKLGFTNKICQESFSSVSSYLIGLPKGKISYSNSGDYHILTYAP
                     SGSTGVDIEKYKDRSEQTYQNYLGESVSSDMDSKLLFYKAWLQKEISYKCGKSIDITY
                     QTMIDGYIYGYAF"
BASE COUNT         4851 a         2073 c         2783 g         4832 t
ORIGIN      
        1 gctgatttaa gctataaaaa tactgttgag taaaagaggt attaatatgt caatgattga
       61 agtttctcac cttcagaaaa attttataaa aaccgttaaa gcaccagggt taaaaggagc
      121 atttcaatca tttcttcgtc ctgaaaaaca cacctttgaa gctgttaaag atttaacatt
      181 tgatgtgcct aaaggacaaa ttttgggatt tattggagcg aacggtgcag gaaaatctac
      241 aacaattaaa atgttaactg ggattttaaa accaacttcg ggcttttgcc gtattgatgg
      301 caaattgcca caagaaaacc gacaaaatta tgttaaggat attggagtcg tttttgggca
      361 acgtacgcag ttatggtggg atttagctct acaagaaacc tatacagtgt taaaagaaat
      421 ctatgacgtt cctgataaag aatttcgcaa acgcatggct tttttaaatg aggttttgga
      481 actaaatgac tttataaagg atcctgttag aactttgtct ctcggacagc gtatgagagc
      541 ggatatcgca gcttctttat tacataaccc aaaggttctt ttcttagatg aacccaccat
      601 tggcttggac gtttcagtga aagataatat ccgacgtgca atcacacaaa ttaatcaaga
      661 agaagagaca acgattttac taacgacaca tgacctcagt gatattgaac aactttgtca
      721 tcgtattttt atgattgata gaggacaaga aatatttgat ggcacagttt ctcagctaaa
      781 agagacattt ggaaagatga aaactctttc ttttgatttg cgcccaggac aagagcatat
      841 cagctctagt ttaattggaa agtcggagat taatattaaa cgtaatgact tagttttaga
      901 tattcagtat gatagttctc gctaccaaac tgctgacata attcagcaga cattagcaga
      961 tttttctgta cgtgacctta aaatgacaga tgcggatatt gaagatatca ttcgtcgctt
     1021 ctatagaaat gagctataag gatgaacaga atttggagac gttataaacc ttttattagt
     1081 acaggaatcc aaggattaat cacatatcgt gttgatttca tcctttatag gataggtgat
     1141 gtgataggag catttgttgc attttatcta tggaaagcag ttttcgattc ctcaagtcaa
     1201 tccttaatcc aaggttttca gctgtcagat atgattctct atattattat gagttttgtg
     1261 actaatttat taacaagaac tgatagttca tttatgatag gtgacgaagt aaaagacggt
     1321 tcaattatta tgcgactctt acgtccagtt cactttgctg cttcctacct tttcatggag
     1381 attggttcta gatggctcat ctttttaagt ataggggtac cgtttttgct tgttattacg
     1441 ggggtacgcc tcttcttggg gactgatttg attcaagcta tagtgttagt ggtattttat
     1501 attattagca ttattttagc ttttttgatt aacttctttt ttaatatttg ttttggcttc
     1561 tcggcatttg tttttaaaaa tttatggggg tcaaacctgt taaaaaattc gttagtcgca
     1621 tttatgtcgg ggagcttgat tcctcttact ttttttccga aaatcgttgc tgatatttta
     1681 ggatttttac cattttcatc tttaatttac acaccagtaa tgattattat tggtaaatat
     1741 gatggcagtc aaattgtgca agccttactt ttacaaatat tttggctaat agtaatggta
     1801 gctttatctc aattaatatg gaaaaaagta cagttgcata tcactatcca aggaggttga
     1861 gatgacaaaa tatcagcgaa tgcactttat ttttattaaa caatacatga agcaaatcat
     1921 ggagtataaa atagactttt ttgtaggcgt tttaggagta tttttaacac aagggttaaa
     1981 tcttttattt ctgaatgttc tttttcaaca tatcccttct ttagaaggat ggacttttca
     2041 gcaaatagct tttatttatg gtttttctct cctcccaaaa ggaattgatc accttttctt
     2101 tgataacttg tgggctcttg gtcaaaggct tattcgaaaa ggagaatttg atgagtattt
     2161 aaccaggccg atcagtcctt tatttcatgt cctagttgaa acctttcaag tagatgcatt
     2221 gggggagtta ttggttggtt ttattctgtt atctaccaca gtatctagta taagctggac
     2281 cgtgccaaaa gtattattat ttatatttat tatccccttt gcaactttga tttatacctc
     2341 cttgaagatt gctacgtcaa gtatcgcttt ttggaccaaa caatctggtg ctgttatcta
     2401 tattttttat atgtttaatg actttgctaa gtatcctgtc gcaatatata ataatctatt
     2461 gcgttggatc attagttttg tcattccatt tgcttttaca gcttattatc ccgctgctta
     2521 cttcttacaa gatagaaatg tctattttaa tattggtggg gttatcttga tttccttgat
     2581 ttcttttatg gtatcactaa tactttggca taaaggagta gaagtatatg agagtgcggg
     2641 ttcttgatta gtaaggcaag ccattaattt ttttgagtgt ttagaagttt taggcttact
     2701 aacttagctt tgttagtgat gatatactcc ccttatagtg tctagtgaat ttttgttttt
     2761 ttactgtcca ctataagggg agtatatcag gtgaagaggg actattagtg ttgagaataa
     2821 tgtccctcat agaacaataa ttaccatatc tttaccaaaa taaagttaaa aacgctctta
     2881 gatgtgcttt ctaagagcgt tttttggtct ataaaaggta agcaaaaagc agatgtcatt
     2941 tatgaaagtg ttgacaaaga tgaaatactt tgttactatt ttaacataat aatgatattt
     3001 taattagagt gtgaatttat aattaatttt tataatgttt attttaaatt taaactaatg
     3061 tttatagcag tgttttactc atgattttgt gaattcaaca cttaagtcat gacctcattt
     3121 taaagagttt cagacgacac agaaaggaag aaaacataag aaaggcaatg cacggcttgt
     3181 ccatagtagt gtttgaagta gatgtttaga aatattttat tttccaacag tactgatatc
     3241 agagcgaaga tgattagttg aaggtgataa tatgggacgt tctaatattt tgagtaggtg
     3301 tgaacaatgt tctgataatc aatgtggctt gtataaaacg cggcttaaag acaagttact
     3361 attagtagtc caactggttg gaaatattag gtattctgag tttcttacgg aaggtggtgt
     3421 atatcataag ggagaaacct tgttggaagt tataacctta ggaatttctt atgaatataa
     3481 aagttatggg gatatcaaag taatcaagaa gtactgtgct gatggcagtg tcgttgatta
     3541 taacatgcca atactattaa tggaaaggat tgaataatga aaacagtaat aatatatgga
     3601 ggacagggta atgttcagtc aaaacacctt tgtcaactag ttaaaactcc aaaaggtgaa
     3661 attctttttt caaagttaca agcaactgat ttagaatcat atcagttaat gatggatgtc
     3721 attaaccaca aggtaagttt agacaatgtt catgcgtcta tgttagcaaa ttttttatat
     3781 aatgattggc taacaaacga tgaaaaaata gtatctggga taggattttc agctcatagc
     3841 gccggtattt tcaatgtgtt atatgctagt ggtagtacta gttttgaaaa cattattcac
     3901 tttattaaac gtcgtgccca acttatagaa aattgtcatg tgtctgaaga gctttggctt
     3961 ttgatggttg aaaacattga tatgtttagt aaggatgttt taaaagaaca gtctgagaaa
     4021 gtaaaattag ctattattac tgatgataga gctggtgtaa ttgctatgga tcaagtcaac
     4081 cgttctttct ttgatgacgt gatgactaac aaaggttatt tttataaatt aaagcagtta
     4141 ggcgtaaaag caccatatca tactgatttt ttaaatgaat ctaaagagca gtataaacaa
     4201 ttgattgaaa gcctagatat ttcgcaaaat gatgactatg actatatttt tcattgtgat
     4261 caattggtag atgaaatttt atatcagtgg accaatctct ttaactggcg tgcgattaag
     4321 gaaactatca tagaaaagga tgcggagatt attgatattt ctcctaatag gtttatttct
     4381 agtcagttgc aaaaaatgaa aaagaggggg aggaaacagt tatgaaagta gcaatagtta
     4441 ctggtggtac cagaggaatt ggaagggcta ttacaaaaga gttatacaaa gaaggttaca
     4501 aagtcattgc tatttataat agcaatgatg ctaaggcaag agctttacaa gaggagttac
     4561 caaaattaga cgtttataaa tgcaatattt cagatgcaaa agcagtacaa aaattggtga
     4621 ctaaaatttt tagagaatat ggcggtattg attgtttagt taataatgct ggtattgtta
     4681 gagatggctt tttcttgatg atgtctaagg aaaaatggat ggatgtcatt aacataaata
     4741 ttatggggct agttaacatg agtaaagccg tgttaaaaat catgaaggct aagcgtatac
     4801 agggaaaggt tatcaatatt tcttcaacta gtggtatagc agggcagata gggcaagcga
     4861 actattcagc aacgaaaggc gctatcattt ccattactaa aactctagcg aaagaatttg
     4921 catcagatgg tattacgatt aattgtgtaa gtccaggatt cattgaaaca gatatgacaa
     4981 atgaattgca aaataaagaa gagctaaaag agcatcttat tcctctaaaa aggtttggac
     5041 aacctgaaga ggtggcttgg ctcgtaagct ttttagcaag tgagaaggca aactatatta
     5101 caggaaaaaa tattgtgatt gatggaggaa tgattaatga ttaactgggc tgaagaacgt
     5161 aaaataataa aaacacgtca agaagtagtt gatgaaatta aaggtatttt gattgactct
     5221 ttaatgttgg atattgataa agatctgatt atgaatgatc aaccattatt tggtcgtgga
     5281 ttagagctag actctattga tgccctagaa ctgtcaatag ggatttcaac aacgtttggt
     5341 gttgaactaa atgatgatga tatttcaata ttatcatcag taaatagact agcagatttt
     5401 gtgattgaaa atagtgagga tatagatgac caagctaagt agtgaagaaa ttataaaaat
     5461 gttacctcat cgctatccgt ttctaatggt ggatagagtg cagagttata aaaaaggtat
     5521 cattgaatgt cgtaagaata ttactatcaa cgaaccttac tttcaaggac attttccaca
     5581 acgtcctata gttccgggtg ttttgatgat tgagatggca gctcaatctg cagctttact
     5641 ttatatttta gatgcacttg atggtagcct cccctctctt gatgaattaa cagcagagaa
     5701 aatagcagaa aaagtaggtt atttagccag tgtgaagaat ttcaaattca agaaaattgt
     5761 tactccagga gaccagctaa acattacatg taaatctcaa tcaaaattgg ggcatctttt
     5821 agaaattcaa gtcgttatta gagatgagaa taaaaaagag gttgcctcag gaaggatgtt
     5881 ggtatcagaa aatggaaatt aaactcaaaa atattggaaa aaaatatggt tcttttgaag
     5941 cattgagaga cattaatctc atctttgaag agggaaaatt ttatggcctt cttggaccta
     6001 atggagctgg taaaacaact ttatttaacc ttttaattca aaattttaag caaacgtcag
     6061 gcgatattaa gtgggaggtg gatggaaaac ctctatctat taaagatttt tatcgtcaca
     6121 ttggtattgt cttccaaagt aatcgcttag atgataattt aacggttgag gaaaatctaa
     6181 tttctagagg tgctttgtac ggtttatcta aatcacaagt acgtaatcgc cttaaagatc
     6241 ttcaaacgta tctagatatc acagcaataa aaaaacaaaa atatggcagt ttatcaggag
     6301 gtcaaaaacg aaaagttgat attgcacgcg ccttgctgcc acaaccttct ctattattat
     6361 tagatgaacc aacgactggt ttagaccctc aatctcgtcg cgatttatgg gatgctatcg
     6421 cacaactaaa tcaacaatcg cagatgacag ttgtactaat tactcattat cttgaagaaa
     6481 tgtctgcttg tgatgtttta aatgttctta ttgaaggaaa catttattat tcgggagata
     6541 ttaaaagctt tattgagcaa cattccacaa caaacttgaa tgtggtattg aaaccagaaa
     6601 agtcattaga ccaactttcg atagctgatt ttgttaataa atgtcaagtc ctttcagaaa
     6661 gggagatagt atttaaggat atttctgttg aggaaatgat gcaggttatc tcagacaatc
     6721 aagataaatc tattattgag acatttaatg ttgaatattc gaacttagag gcggcgtatc
     6781 tcaacttgct tagagaaaaa gaaaggaaaa acaatgtttg aactaatttc tagaaatcgt
     6841 aaagtttaca cgcgagatcg gttagcgttt ttcatgtctt ttttatcagt tattatctta
     6901 attttggtct atcagatttt cttgggaaaa attcaattag atgctataaa agcagcattg
     6961 aggagtaata cagtatccag tgatactatt cacatggtta attattggct cattgcaggt
     7021 ttgacgacta ttatttcaat gactagtaca ttaggtgcct ttggagttat ggtgtccgac
     7081 aaagaaaaaa aattaaatga agattttaag gtcagtccta tatcaaatat caaaattgaa
     7141 ttgtcctatg ctatatttgc cgttttattt ggtattgtca tgacgatgtt ctcttgcatc
     7201 tttgctatcg gtatttttaa cggctttaat tccttgctag agtattcagc tatggattat
     7261 ttaactattt tagggatcgt ttccttaggg actgttttat ctgcggcgat gattttacct
     7321 attttagcct ttattcatac tagttcagct tttacgactc ttagcacaat tgtagggact
     7381 tttattggtt ttatttcagg agtatatctc tcaattggct cggtaggaaa agcattacaa
     7441 caggttatga catggtttcc attgactcaa atcaattctc ttttgaaaca ggtcttaatg
     7501 aagggttcta ttgcgaaggt atttgacaaa gccaacgaag ccactgtctc taactataaa
     7561 gaatcatatg gtgttgtttt gcgtaatgct gatggagaaa ggctgagtaa tcactttatg
     7621 ttgatttata tcattgccct cattcttatt ttattggcaa ttcattttat tattaaaaag
     7681 gttaaaaaat gaaagatgat aataaattaa agatttctga agcttcctta gaagattatt
     7741 ctgaagtggt tcatttattt aataggaatc atgtttatca atttccagac ggtaggcctt
     7801 taactgttga tgaccttgat ttaacgttaa aagttaaaga agtgacacat ttatttttac
     7861 tgaaaaatca tggtgttttg attggaacat cagctttctt taaatttatt acctatggtt
     7921 gtttagattg gaatagtagc tttagtggtt ttcttttaat tgattcgaaa agtcgtagtg
     7981 gacaggcaat cacttatcta tataaaacta tcttaaagaa gataacaaaa ctgaagtttt
     8041 ctaatatcta taccgaaatc agtaactaca ataaaccctc cttagcttta tcaaaattga
     8101 atggcttcaa agagtatgat aagacatatg aagacatact gcattgtcga tcattgcgta
     8161 gtcacctccc taagatattg aatacgtttc gtatttcgaa ttattatggt aaaacatacg
     8221 acatatcaac ttttcaaatt atggaggaaa ttgaaaatcc cttggaagag gagacagaaa
     8281 ttaggaccaa agtctctgat gaagaaatat tatttaaagc agaagatagt gcttcacttc
     8341 cttattattt aaaaatgagc ttgtttcaaa tggaaattgc taagttagat aaccgttatg
     8401 ttttacaagt tgacttttta tcggaacaag taaagagggt tcgggttaaa actggaaaat
     8461 atcacttggc aaacttaacg agggctcacc ctagcctaac tttaagtcgt tttgccaatt
     8521 attattacat tcaagcaact gtggaaaccc tttatggtaa tatcgatgtt caactggaac
     8581 gtcgaaaaaa gcattataga gatgcaacta tttgtcttaa gagaactttt caaggttatg
     8641 atttacttat ctctccaaat ggcagcctta tttttgaaaa gcagaaaaga aagatacttg
     8701 aagatagttt tttgattttt agccaacctt tggacaagaa attagttgta aaagaagaag
     8761 aaaatcacat tacaatcaaa tgtttctatc aaggtgcttt aattgagaaa atcatgacat
     8821 ttacaagtga cgaagaaatc acttgtgttt ataaatgtaa tcaaaaggct aaagagatgt
     8881 tcccaaaatt attgaaacag actttcaagt tacactgcca agagcagttg attagagata
     8941 gtgaaggtta tcttgtgaat gttccaggaa gttacccgat tgagcatgat gattttcttc
     9001 gagcagataa gtttgaagat agacaattcc attattacct cccgaatgaa gataaaatga
     9061 tttcctattc tcctcctggc aaagccagca atcaaatgca atttagaccc ttatgtctta
     9121 ttgatacaga tagcctttct tttccactga cttatcattt taggatttca caagcatcat
     9181 cggaggaggc gttaatcaat ctaaaaaaac agccaatatg ggacagcaac taccaagcgt
     9241 cagcaactga ccttttaaaa catatctcta acctaacatt agaagaagag aaggattatg
     9301 gcatcaagag aatgatagct aacagaaagc attatcctag tcacaaactg gttttagcct
     9361 ataatcagat tgttttgcct aagaatgaaa ttccaagaga cagtgaatta tattctattt
     9421 cttttgatta tcgaattaga ggaaagtttg tccaaatccg ccaaggggaa catgtcaaat
     9481 atgataataa atcctatgtt ttggaaaata gtcaacaatt agtattgtat gtagctagcg
     9541 atgataaata tatactaatt tctgctaaaa atggaatatt ttattcttac aaagagaaca
     9601 atcatttaaa aataaggtgc atgtttaaaa ggaactcacc ttatgctaca aatgtgagca
     9661 ttacagaata tagaaagtgt gaagaaaaat gaatactatt tataatacat tgagaacaga
     9721 taaaggttat aaagtttatg aggggtattt atatgaaatt actggtgaag aatgtgaaga
     9781 agccttagac cttgtgattc ctaagaatat tgtatttgca gatacagata cttgtggcta
     9841 cactttttta ctcaatgaag atggaacagt ttatgatgat gtgactttct acaaatttga
     9901 tgataaatat tggttggcta gtcataaagc tttggattct tatttagaca acatcaattt
     9961 tgactatacc gtaacagata tttctgacga gtataaaatg ctgcaaattg aaggaagata
    10021 ttcgggagaa attgctcagt cattttatga atatgatatt tcaacactta attttcgtac
    10081 tttgatagag atgacttata aaggtgagaa aggttatctt gctagatttg gtttttctgg
    10141 agaatttggc tatcaatttt tcctaccatc ttctattttt gctacttttg tttcggatgt
    10201 ctgtgaaggt atagcagagt atggggatga acttgataga tatttaaggt ttgaagtggg
    10261 acaacccatt actgatattt atcaacaaga agaatattct ttatatgaaa taggttattc
    10321 ttggaatcta gatttcacaa aggaagaatt tagaggtcgc gatagcttgt tagagcacat
    10381 cagatcagca acagttaaaa gtgttggatt ctcaacgaag gaaaaactcg cttcaggaac
    10441 accagtgcta tttgatgacc aaattgttgg aaagattttt tggatagcag acgagaaaga
    10501 ctcttcggaa aattacctag gtttgatgat tgttaaccaa acatatgctc attcaggagt
    10561 tacttttgta acagaagatg gccaaatttt gaaaacacaa tcaagccctt attgtatccc
    10621 agaaagttgg aacaaagaat gagcgtatat gttagtggaa taggaattat ttcttctttg
    10681 ggaaagaatt atagcgagca taaacagcat ctcttcgact taaaagaagg aatttctaaa
    10741 catttatata aaaatcacga ctctatttta gaatcttata caggaagcat aactagtgac
    10801 ccagaggttc ctgagcaata caaagatgag acacgtaatt ttaaatttgc ttttaccgct
    10861 tttgaagagg ctcttgcttc ttcaggtgtt aatttaaaag cttatcataa tattgctgtg
    10921 tgtttaggga cctcacttgg gggaaagagt gctggtcaaa atgccttgta tcaatttgaa
    10981 gaaggagagc gtcaagtaga tgctagttta ttagaaaaag catctgttta ccatattgct
    11041 gatgaattga tggcttatca tgatattgtg ggagcttcgt atgttatttc aaccgcctgt
    11101 tctgcaagta ataatgccgt aatattagga acacaattac ttcaagatgg cgattgtgat
    11161 ttagctattt gtggtggctg tgatgagtta agtgatattt ctttagcagg cttcacatca
    11221 ctaggagcta ttaatacaga aatggcatgt cagccctatt cttctggaaa aggaatcaat
    11281 ttgggtgagg gcgctggttt tgttgttctt gtcaaagatc agtccttagc taaatatgga
    11341 aaaattatag gtggtcttat tacttcagat ggttatcata taacagcacc taagccaaca
    11401 ggtgaagggg cggcacagat tgcaaagcag ctagtgactc aagcaggtat tgactacagt
    11461 gagattgact atattaacgg tcacggtaca ggtactcaag ctaatgataa aatggaaaaa
    11521 aatatgtatg gtaagttttt cccgacaacg acattgatca gcagtaccaa ggggcaaacg
    11581 ggtcatactc taggggctgc aggtattatc gaattgatta attgtttagc ggcaatagag
    11641 gaacagactg taccagcaac taaaaatgag attgggatag aaggttttcc agaaaatttt
    11701 gtctatcatc aaaagagaga atacccaata agaaatgctt taaatttttc gtttgctttt
    11761 ggtggaaata atagtggtgt cttattgtca tctttagatt cacctctaga aacattacct
    11821 gctagagaaa atcttaaaat ggctatctta tcatctgttg cttccatttc taagaatgaa
    11881 tcactttcta taacctatga aaaagttgct agtaatttca acgactttga agcattacgc
    11941 tttaaagggg ctagaccacc caaaactgtc aacccagcac aatttaggaa aatggatgat
    12001 ttttccaaaa tggttgccgt aacaacagct caagcactaa tagaaagcaa tattaatcta
    12061 aaaaaacaag atacttcaaa agtaggaatt gtatttacaa cactttctgg accagttgag
    12121 gttgttgaag gtattgaaaa gcaaatcaca acagaaggat atgcacatgt ttctgcttca
    12181 cgattcccgt ttacagtaat gaatgcagca gctggtatgc tttctatcat ttttaaaata
    12241 acaggtcctt tatctgtcat ttcgacaaat agtggagcgc ttgatggtat acaatatgcc
    12301 aaggaaatga tgcgtaacga taatctagac tatgtgattc ttgtttctgc taatcagtgg
    12361 acagacatga gttttatgtg gtggcaacaa ttaaactatg atagtcaaat gtttgtcggt
    12421 tctgattatt gttcagcaca agtcctctct cgtcaagcat tggataattc tcctataata
    12481 ttaggtagta aacaattaaa atatagccat aaaacattca cagatgtgat gactattttt
    12541 gatgctgcgc ttcaaaattt attatcagac ttaggactaa ccataaaaga tatcaaaggt
    12601 ttcgtttgga atgagcggaa gaaggcagtt agttcagatt atgatttctt agcgaacttg
    12661 tctgagtatt ataatatgcc aaaccttgct tctggtcagt ttggattttc atctaatggt
    12721 gctggtgaag aactggacta tactgttaat gaaagtatag aaaagggcta ttatttagtc
    12781 ctatcttatt cgatcttcgg tggtatctct tttgctatta ttgaaaaaag gtgataaaat
    12841 gagtaaattt ttatttgctc ctttcatgat gaatttagga gagagccaaa gattatcaaa
    12901 actggctaat tgtttatatg aacaaggaca tgatattcat atccttggag acaattatta
    12961 tccattttta tttaataatg atgcttatcg ctatcaccac tgtgtagaag ataaatctgt
    13021 ttataatagt aagcgatatg atgctttctt ttctctttca acagacttta attttttatc
    13081 agaagaagaa attgaacgca tctgttctat tgaaagagac ttgttacgcc aagaaaaatt
    13141 tgatgctgta ttgacaggtt accgtctatc tattgttaca agttgtcgtt tagaaagcat
    13201 accattaatt tggattattt caggggcaac gcatatcagt gaaattgttg aaaactcaga
    13261 aggaatattg cctaatggga agataagtaa agctagtaag cctcaaacaa aggattttat
    13321 caaaagagta atcattactt attcaacaaa tgttaaaacg tggaataact atattaaaaa
    13381 atatggtggc aaacctttta ataatgcttt agaattattt actggtgatc taaacttggt
    13441 aacagattac tctttgtttt atgaatttga taaagattcg tcctataaaa cgataggacc
    13501 tattttgata gataatgtag gtttttcaaa atgcagccaa attaatcaag acaataagac
    13561 tgtactgctc agctttggta cttcatttaa acgagattgg gtggaatctt ttttaaagac
    13621 attaccaagg cattatcatt atttactgac aagctgtggt gaggctataa acattccagg
    13681 aagttatatt gagatagttg attttattga ttttaaaacc atagataaag agatttattt
    13741 cgctattata cacggtgggc aaggaacagt ttatgcgatg gctgcacaag gtattccctt
    13801 tataggtatc cctttcttta acgaacaatt ttggaatatt aagaagttca gtaaagaaaa
    13861 gtgcgcttat ttggtaaaga aacctgatgt aagagagata cgtaaagcaa ttagctgtct
    13921 tgaaagagac tatgatatct ataaaagtta tatgataaga ctaagccaag gaattaaaga
    13981 agaagcagag gcatctttac aaaaagcgga ggcagaaatt gaatccttta tcagaaaaag
    14041 acaaagttaa ctatattatg atttataaaa ataggcagtc tacattggag aataatttat
    14101 ctggcgatcg gttgatgaga tatcaacaag caaatagaca ggtaagcaaa gataaactgt
    14161 taacagggag ttattttgtg taccaatgct taaaaaagtt aggttttacc aataagattt
    14221 gtcaggaatc tttttctagt gttagtagtt atttaattgg tttgccaaag gggaaaatta
    14281 gttactctaa ttctggtgac tatcatattc taacctatgc tcccagtggt tcaactgggg
    14341 ttgatattga gaaatacaaa gatagatcag aacaaaccta ccaaaactac ctaggagaat
    14401 cagttagtag tgatatggat tctaaattat tattttataa ggcatggtta caaaaagaaa
    14461 tttcctataa atgtgggaaa tctatagata ttacctatca aacaatgata gatggctata
    14521 tttatggtta tgcttttga
//
LOCUS       DATRMA010000043        14342 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.043, whole genome shotgun sequence.
ACCESSION   DATRMA010000043 DATRMA010000000
VERSION     DATRMA010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14342
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.043"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            349..981
                     /locus_tag="VBC28_001298"
     CDS             349..981
                     /locus_tag="VBC28_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000284161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P"
                     /protein_id="HEO6815281.1"
                     /translation="MYQELNDYLEFQSMKYIMPEKAGPLKDDMQTFKAKGQLARNSFT
                     ELSRALEQRMDGFKMQRVSNWANQAQVGRPHFWVYYRKDTDQLDDVAVALRVYGVKDS
                     FGVSLEVSFVERQKSDKTLEKQARVLSIPIASPLYFMVQRQGETHREEGNEENRQRLM
                     QEIKSGKVRKVLVKYDVLLTENQSLENILQRLLEGFEKVLPYYQVCQTIS"
     gene            complement(1027..2304)
                     /gene="serS"
                     /locus_tag="VBC28_001299"
     CDS             complement(1027..2304)
                     /gene="serS"
                     /locus_tag="VBC28_001299"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEO6815282.1"
                     /translation="MLDLKRIRTDFDVVAKKLATRGVDQETLTTLKELDIKRRELLIK
                     AEEAKAQRNVASAAIAQAKRNKENADEQIAAMQTLSADIKAIDAELADVDANLQSMVT
                     VLPNTPADDVPLGADEDENVEVRRWGTPREFDFETKAHWDLGESLGILDWERGAKVTG
                     SRFLFYKGLGARLERAIYSFMLDEHAKEGYTEVIPPYMVNHDSMFGTGQYPKFKEDTF
                     ELADSPFVLIPTAEVPLTNYYRDEIIDGKELPIYFTAMSPSFRSEAGSAGRDTRGLIR
                     LHQFHKVEMVKFAKPEESYQELEKMTANAENILQKLNLPYRVITLCTGDMGFSAAKTY
                     DLEVWIPAQNTYREISSCSNTEDFQARRAQIRYRDEVDGKVRLLHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTIPEVLRPYMGNIDIIKPN"
     gene            2601..3662
                     /locus_tag="VBC28_001300"
     CDS             2601..3662
                     /locus_tag="VBC28_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEO6815283.1"
                     /translation="MEKYTSIKQSGRHYGLDLLRIISMFMIVITHVLGKGGLRSSVEG
                     HADSYFIVTWIIQVLVYGAVNCYALISGYVGINSRYRYSKLLSIWAQVFFYTFTITAL
                     FAITGHEVTLLNWRDAFFPIVSGQYWYITAYFGLLVFMPVINNGLNALTDKQLKQLVL
                     LMFIIFSILPAVLNNRVPEFSLSKGFEMTWLLILYIIGAYLKRIDLNIFKTSYLLIIY
                     LLSLVATYAMKFSVGDIWYWYVSPTLTLGAVSLFILFARASIKPSGFLKKIIVVLAPS
                     TLGVYLCHLHPLIVKYFVRDFAETFVYESIYLYPFLILGAGILIYLLAFIVERIRLRI
                     FKAIHFSNFLRWFDDKFPY"
     gene            complement(3700..4062)
                     /locus_tag="VBC28_001301"
     CDS             complement(3700..4062)
                     /locus_tag="VBC28_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HEO6815284.1"
                     /translation="MAQSLNKTVEFQTTGVSYLGMGNKVGKFLVGDQALEFYNDKNVN
                     DYIQIPWTSINQIGANVSRKKISRHFEVFTDQGKFLFASKDSGTILKHARRHIGDDKV
                     VKLPTLIQTILKIFKFKK"
     gene            complement(4181..5092)
                     /locus_tag="VBC28_001302"
     CDS             complement(4181..5092)
                     /locus_tag="VBC28_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000139230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEO6815285.1"
                     /translation="MTEQIKLSKSDRQKVWWRSQFLQGSWNYERMQNMGWAYALIPAL
                     KKLYTTKEDRAAALERHMEFFNTHPYVAAPIIGVTLALEEEKASGTPVEDKAIQGVKI
                     GMMGPLAGIGDPVFWFTVRPILGALGASLASAGNILGPIIFFVGWNLIRMSFLWYTQE
                     LGYKSGKEITKDMSGGILQDITKGASILGMFILAVLVKRWVAINFTVDLPKKTLSEGA
                     YINFPKDHVSGQQLHDILGQVQSGLSLDKMQPQTLQGQLDSLIPGLAGLLLTFFCMWL
                     LKKKVSPITIIIGLFIVGILARLAGVM"
     gene            complement(5107..5919)
                     /locus_tag="VBC28_001303"
     CDS             complement(5107..5919)
                     /locus_tag="VBC28_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HEO6815286.1"
                     /translation="MADISIISMILVVIVAFFAGLEGILDQFQIHQPLVACTLIGLVT
                     GHLEAGIILGGSLQMIALAWANIGAAVAPDAALASVAAAIILVKSGDFTQKGINFAFS
                     TAVPLAIAGLFLTMIVRTISTALVHAGDKAASEGNFAAIERFHFIALLLQGLRIAFPA
                     ALLLAIPSSSVQSILEAMPDWLNGGMQVGGAMVVAVGYAMVINMMATREVWPFFALGF
                     ALAALNQLTLIAMGTIGVAIALIYISLSKMGGSKGTSNAGSNDPIGDILEDY"
     gene            complement(5952..6962)
                     /gene="manX"
                     /locus_tag="VBC28_001304"
     CDS             complement(5952..6962)
                     /gene="manX"
                     /locus_tag="VBC28_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000511108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HEO6815287.1"
                     /translation="MGIGIIIASHGKFAEGIHQSGSMIFGEQEKVQVVTFMPNEGPDD
                     LYGHFNNAIAQFDADDEVLVLADLWSGSPFNQASRVMGENPERKMAIITGLNLPMLIQ
                     AYTERMMDANAGVEQVAANIIKESKEGIKALPEELNPVVEATPVAGVPADVPAEVKQS
                     GSIPEGTVIGDGKLKINLARIDTRLLHGQVATAWTPASKANRIIVASDEVSKDELRKQ
                     LIKQAAPGGVKANVVPISKLIEVAKDPRFGNTRALILFETVQDALRAIEGGVEIPELN
                     VGSMAHSTGKTMVNNVLSMDKDDVAAFEKLRDLGVSFDVRKVPNDAKKNLFDLINKAN
                     VK"
     gene            complement(7265..8077)
                     /locus_tag="VBC28_001305"
     CDS             complement(7265..8077)
                     /locus_tag="VBC28_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEO6815288.1"
                     /translation="MTKKIIAVDLDGTLLHNNNTISDYTADTLRKVQAQGHKVIITTG
                     RPYRMALAHYLRLDLKTPMINFNGALTHIPEKKWAFERSATIDKKLLLETLNLSDAIQ
                     ADFIASEYRKNFYITMDNRDKINPQLFGVNEITDKMALDVTKITRNPNALLMQTRHKD
                     KYELAKELRQHFNHELEVDSWGGPLNILEFSPKGVNKAYALKHLLKSLNLSQENLIAF
                     GDEHNDTEMLAFAHTGYAMKNANPTLLPYADQQIQWTNEEDGVAKTLEKLLL"
     gene            8166..8750
                     /locus_tag="VBC28_001306"
     CDS             8166..8750
                     /locus_tag="VBC28_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000734041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6815289.1"
                     /translation="MKKLIWSHLFAIVSILGIWYYHVKIDHLMVILLASVLSLEMGFI
                     AYYSRNIFIRLLASLGLLFFYPQSLGIFLSLVSLEWSTKDIWQSNHFMQYMAYLLAIL
                     FIISSALLSLKLLVNAYDISKRYQLATVIPVMLFSSFAFCLEKSGSLGLEGIILNPSA
                     FFNGIQTNMANLDFKALSCLFLVQLLLWGILMED"
     gene            8840..9451
                     /locus_tag="VBC28_001307"
     CDS             8840..9451
                     /locus_tag="VBC28_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1361 domain-containing protein"
                     /protein_id="HEO6815290.1"
                     /translation="MKVKLFAIHLFFIVISFGIKYYHLQGPNLIWNMTLALIALDFAY
                     LTSLFKKKILIGLFALAWFFFYPNTFYMLTDIIHMHFVGDVLYNKTNLILYILYVSSI
                     LFGFLSGIESFSVIMRKFRISNIFLRWGIIGIVSFVSSFGIHIGRYARLNSWDILTKP
                     QVVINELLAVPSRDSFHFILGFTFLQVLCLIFMDYSAGKKLEK"
     gene            9554..10975
                     /locus_tag="VBC28_001308"
     CDS             9554..10975
                     /locus_tag="VBC28_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEO6815291.1"
                     /translation="MEKFFKLKEHGTTIRTEITAGLTTFFAMSYILFVNPAILSQTGM
                     PAQGVFLATIIGAVVATSVMAFYANLPYAQAPGMGLNAFFTYTVVFALGYTWQEALAM
                     VFICGLISLIITLTKVRKMIIESIPTTLKSAITAGIGTFLAYVGIKNAGFLKFSIDPG
                     TYDVVGKGAAKGLATITANSSATPGLVSFDNPAILLSLIGLSITIFFIVKGIRGGIIL
                     SILTTTLLGILMGVVKLDAINWEATNLSASFRDLKQVFGVALGEKGLISLFSNPSRLP
                     SVLMAILAFSLTDIFDTIGTLIGTGEKVGILATTGDNHESKSLDKALYSDLIGTTFGA
                     ICGTSNVTTYVESAAGIGAGGRTGLTALVVAGLFAISSFFSPLVSIVPSQATAPILVI
                     VGIMMLSNLKDIKWDDMSEAIPAFFTSLFMGFTYSITYGIAAGFLTYTLAKVIKGQAK
                     DIHVVLWILDILFILNFISLAIL"
     gene            11058..11567
                     /gene="tsaE"
                     /locus_tag="VBC28_001309"
     CDS             11058..11567
                     /gene="tsaE"
                     /locus_tag="VBC28_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000964341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEO6815292.1"
                     /translation="MLWGCQNQQTVGRQRYFWYNISMFYTQNEEELIALGQKLGTVLK
                     SGDIVLLTGNLGAGKTTLTKGIAKGLDIKQMIKSPTYTIVREYEGRVPLYHLDVYRIG
                     DDPDSIDLDDFLFGQGVTVIEWGELLSDNLINNYLEIVITRSNQGRQVQLEAYGHRAR
                     EIIEAIQDV"
     gene            11521..12081
                     /locus_tag="VBC28_001310"
     CDS             11521..12081
                     /locus_tag="VBC28_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001890427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO6815293.1"
                     /translation="MGIAQEKLLRRFKMSKQFAVDFIEASREEASAILEFLNTVTEET
                     DFILHTVSNQLSLSEMETFIENTLMTKNCICLIAKLKNKVIGLITIISQSDIEIEHVG
                     DLFIAVQKDYWGYGIGHILMEEAIEWASDNDITRRLELSVQGRNERAIHLYQKFGFEI
                     DGLQIRGIKRENGEFLDIYRMSKLID"
     gene            12090..13397
                     /locus_tag="VBC28_001311"
     CDS             12090..13397
                     /locus_tag="VBC28_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000708155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEO6815294.1"
                     /translation="MKIWKKITLMFSAIILTTVIALGVYVASAYNFSTNELSKTFKDF
                     KLAKSKSHAIEETKPFSILLMGVDTGSEHRKSKWSGNSDSMILVTINPKTNKTTMTSL
                     ERDVLIKLSGPKNNGQTGVEAKLNAAYASGGAEMALMTVQDLLDINVDYFMQINMQGL
                     VDLVNAVGGITVTNKFDFPISIAANEPEYKAVVEPGTHKINGEQALVYSRMRYDDPEG
                     DYGRQKRQREVIQKVLKKILALNSISSYKKILSAVSNNMQTNIEISSKTIPNLLAYKD
                     SLEHIKSYQLKGEDATLSDGGSYQILTKKHLLAVQNRIKKELDKKRSKTLKTSAILYE
                     DYYGTTASNDSSTYSSTQENNYNTTPYSEAPPSYSGNTTYSSETNQTTHQNYYNSSTP
                     ASNYSSNTNTGQADSSGSVNNHNGAATPNPNTGTQPVPGQTNP"
     gene            complement(13461..13757)
                     /locus_tag="VBC28_001312"
     CDS             complement(13461..13757)
                     /locus_tag="VBC28_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6815295.1"
                     /translation="MKIGRLIALGLVSLGALELYKNRKTIKDSYQNTKNETDSAKLKL
                     ERIKNDLAIISQEKEKIRLISQELNHKFQVFNKDIQPRLEEINQRMAKYQEKDE"
     gene            complement(13754..14173)
                     /locus_tag="VBC28_001313"
     CDS             complement(13754..14173)
                     /locus_tag="VBC28_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEO6815296.1"
                     /translation="MDNCIFCKIISGEIPSSKVYEDDEVLAFLDITQTTTGHTLLIPK
                     KHVRNVLEMDEKTAQITFERLPKVARAVQAATKAKGMNIINNNEEIAGQTVFHAHVHL
                     VPRFDESDGIKIHYTTHEPDFEALAKLAKEIRKEIQL"
BASE COUNT         4465 a         2353 c         2718 g         4806 t
ORIGIN      
        1 ccaattttta gattgtagtc taatccaaaa ttttcagaaa gagtcatttc gaacacttta
       61 ttctaagtac cattatttag gctctcctta acttgagact ctatctgtta ttgaagagaa
      121 attttctaga aaaaatagct attattagat tttagaaggc tatgaaaaga ttggttttta
      181 aagagttcca ttaagtgatg aagaaagtaa agcttggtta gttataattg cgattagacc
      241 ttgtttctag caacgaggct tcgctagaaa tactttaaaa gaacttgaaa gtatgtatag
      301 atatatataa tcgtgaaaac ttaactattg tttacatgga gaaaaagaat gtatcaagaa
      361 ttaaatgatt atttagaatt ccaatcaatg aagtatatta tgcctgagaa ggcaggccct
      421 ttaaaggacg atatgcaaac ttttaaggca aagggccaac ttgctaggaa tagctttaca
      481 gagttatcaa gagccttaga acaacgtatg gatggcttta aaatgcaaag agtgagtaat
      541 tgggctaatc aagctcaagt agggcgtcca catttttggg tttattatcg aaaggataca
      601 gaccaattag atgatgtggc tgttgcgcta agggtttatg gtgtaaaaga tagttttggg
      661 gttagccttg aggtaagctt tgtagagagg caaaaatctg ataagacact cgagaagcag
      721 gctagagtgt tatcgattcc gattgcctct ccattgtatt ttatggtcca aaggcaaggt
      781 gaaactcatc gagaagaagg caatgaggaa aatcgtcaac gtttaatgca agaaattaag
      841 tcagggaagg taaggaaagt attggtaaaa tatgacgttc ttttgactga aaaccagagc
      901 ttagaaaata ttctgcaacg attgttagaa ggctttgaga aagtgttacc ttactatcaa
      961 gtttgtcaaa ctattagcta agttcataga aaaaatgagt tggtatacca actcatttta
     1021 gagtgcctaa tttggtttaa taatatcgat attacccata tatggacgaa gaacttctgg
     1081 gatagtcaca gaaccatctt cattttgata attttcgagg attgctgcta cagtacgtcc
     1141 aactgcaagt cctgaaccat ttaaagtatg aagcaaccta actttgccat caacttcgtc
     1201 acggtaacga atttgagcac gacgtgcttg gaaatcttca gtattagaac atgaagaaat
     1261 ttctcgataa gtattttgag cgggaatcca tacctctaag tcatacgttt tagctgctga
     1321 gaatcccata tctcctgtac acaaggtaat gacacggtat gggagattaa gtttttgaag
     1381 aatattttca gcatttgctg tcattttttc taattcttgg tatgattcct caggcttggc
     1441 aaatttcacc atttctactt tatggaattg atgaaggcga attaatccac gtgtgtcacg
     1501 tcctgcagag cctgcttctg aacggaatga tggactcatg gcagtaaagt atataggtaa
     1561 ttctttccca tcgataattt catcacgata atagtttgtt aaaggaactt cagctgttgg
     1621 gataagtacg aatgggctat ctgctaattc gaaagtatct tccttgaact ttggatattg
     1681 tcccgtaccg aacattgaat cgtggttaac catataagga gggatcactt cagtgtaccc
     1741 ctccttagcg tgttcatcca gcatgaagct gtagatagca cgctctaaac gagcacctag
     1801 acctttgtag aagaggaagc gagagccagt aacttttgca ccacgttccc aatcgagaat
     1861 tccaaggctt tcacctaaat cccagtgagc tttggtctca aaatcaaatt cacgaggagt
     1921 tccccagcga cgaacttcaa cattttcatc ctcgtctgct ccaagcggca catcatcagc
     1981 tggtgtatta ggaagtacgg tcaccattga ttgtaaatta gcatccacat ctgccaattc
     2041 cgcatcaatt gctttgatat cagcggaaag tgtttgcata gctgcgattt gctcgtctgc
     2101 attctcttta tttcgttttg cttgagcaat ggcagcagaa gcaacattac gttgcgcttt
     2161 cgcctcttca gctttgataa gtagttcacg gcgttttata tcaagttctt taagggttgt
     2221 taatgtttct tggtcaacac cacgcgtggc caatttttta gcgacaacat caaaatctgt
     2281 acgaatacgt tttaagtcta acatatagga atctccttta tactaaaaaa caccctgtca
     2341 tgctggagcg caaaggcgcg gttccatcca gcttcacaga agtgcacttg attgattgat
     2401 taaattattt cccataaatg gaacacggta gaatttcata aaacaatctt atctgctcgc
     2461 aacgaccgca gactctctga aaagaactat tatactactt atccgttaag gttattatat
     2521 ccaaaaataa gtaaaatgta aaccttaact ttataagcct gctctttgtt ttagtgaaat
     2581 agctatgcta aaatatgatt atggaaaagt atacaagtat taaacaatca ggtcgtcatt
     2641 acggcttaga ccttttaagg attatttcca tgttcatgat tgttattacg catgttcttg
     2701 gaaaaggtgg tctgagatct tcggttgagg gacacgcaga ctcctatttt atagtaacgt
     2761 ggattatcca agtcttagtt tatggggccg ttaactgtta cgcactaata agtggttatg
     2821 tgggaattaa ttcgcgttat cgttactcaa aattactgtc tatctgggca caggtttttt
     2881 tctacacctt tacaataaca gcactttttg caatcacagg tcatgaagtg accttgctta
     2941 attggagaga tgcttttttc cctattgtca gtgggcagta ttggtacatt actgcttact
     3001 ttggtctatt ggtttttatg cctgttatta ataatggatt aaatgccttg actgataaac
     3061 aattaaaaca attggttttg ttaatgttta ttattttttc aattttacca gctgttttaa
     3121 ataatcgtgt tccagaattt tcattgagca agggttttga aatgacttgg ctactgattt
     3181 tatatattat tggtgcctat ttgaaacgta ttgatttaaa tatatttaaa acgtcatatt
     3241 tacttataat ttatctatta tcattggttg ctacttatgc catgaagttt agcgttggtg
     3301 atatttggta ttggtatgtt tcgcctactt taactttagg ggcggtaagt ttgtttatat
     3361 tatttgctag agcaagcatt aagccctcgg gattcttaaa gaaaataatt gttgtattag
     3421 ccccttcaac cttgggagtt tatctctgtc atctccaccc actaatagta aaatattttg
     3481 taagagattt tgcagagacg tttgtttatg agtccattta tctttaccca tttttaattt
     3541 taggagcagg gattttaatt tatttgttgg cttttattgt agaacgtatt agattacgaa
     3601 tatttaaagc aatccatttt tctaactttt tgagatggtt tgatgacaag ttcccatatt
     3661 aatattagag ttaaaaaata agatagacta tagtctatct tattttttaa acttgaatat
     3721 ttttaaaatg gtctggatga gggtaggaag cttaacaact ttatcgtctc caatatgtcg
     3781 tctcgcatgc tttaatatcg ttccagagtc tttagaggcg aataaaaatt ttccttggtc
     3841 tgtaaagacc tcaaagtgac gactaatttt ttttcgacta acattagctc caatttgatt
     3901 gattgatgtc catggaattt gaatatagtc gttgacattt ttatcgttat agaattctaa
     3961 tgcctggtca ccaactaaaa attttccaac tttattcccc atacctaaat acgaaactcc
     4021 tgttgtttga aattcaacag ttttgtttaa tgattgtgcc atgactaatc cctttttaat
     4081 ttatagtagt ttgattatat cataatagtg ggactctaag tggattttat gtaataaaat
     4141 ataagagatg gaatactatt gtaaccatct cttagtttta ttacatcaca cctgcaaggc
     4201 gagctagaat accaacgata aacaaaccga tgataattgt aattggtgat actttcttct
     4261 taagtaacca catacagaag aatgtaagaa gtaatccagc taaaccagga ataagtgagt
     4321 caagttgtcc ttgaagtgtt tgtggttgca ttttatcaag gcttaagcca ctctgaactt
     4381 gtcctaggat atcatgcaat tgttgtccac taacatggtc tttagggaaa ttgatgtatg
     4441 ccccttctga caatgtcttt ttaggaaggt ctacagtaaa gttaattgct acccaacgct
     4501 taacaaggac agcaaggatg aacataccaa ggattgaagc acctttagta atatcttgga
     4561 ggataccacc agacatatct ttagtaattt ctttacctga tttgtaacct aactcttgtg
     4621 tgtaccataa gaatgacata cgaatgaggt tccaaccaac aaagaagatg attggtccga
     4681 ggatattacc agcagaggca agtgaagcac caagagcacc taagatagga cgtacagtaa
     4741 accagaatac tggatcacca ataccagcaa gaggtcccat cataccgatt ttaacccctt
     4801 gaattgcttt gtcttcgaca ggtgtaccgc ttgctttttc ttcctcaaga gcaagggtta
     4861 caccgataat tggagcagca acgtatggat gtgtgttaaa gaattccata tggcgctcaa
     4921 gcgcagcagc acgatcttct ttagtagtgt aaagtttttt caatgctggg ataagagcat
     4981 aggcccaccc catattttgc atacgctcat agttccaaga accttgaagg aattgtgaac
     5041 gccaccaaac tttttgacga tctgattttg ataatttaat ttgttcagtc attgtttagc
     5101 tcctttctag tagtcctcta agatatcgcc gataggatcg ttagaacctg cgttagaagt
     5161 acctttgctt ccacccattt ttgataaact gatgtagatg agggcgatag caacaccaat
     5221 agtacccatt gcaataagag ttaactggtt caaagctgct agagcaaatc caagagcaaa
     5281 gaaaggccaa acttcgcgag tagccatcat attgataacc atagcgtaac caacggcaac
     5341 aaccatggca ccaccaactt gcataccacc atttaaccaa tcaggcattg cttcaagaat
     5401 actttgtact gaagaagatg gaatagctaa aagaagcgca gctgggaatg caatacgtaa
     5461 tccttggaga aggagggcga taaagtgaaa acgttcaatt gctgcgaagt ttccttcaga
     5521 agcagctttg tcaccagcgt gaacaagtgc agttgagatt gtacgaacaa tcattgtaag
     5581 gaaaagtcca gcaatagcta gaggtacagc agtagagaag gcaaagttga tacctttttg
     5641 agtgaagtca ccacttttaa ccaaaataat agcagcagca acagaggcaa gtgcagcgtc
     5701 tggtgctacg gcagctccga tatttgccca agcaagagca atcatttgga gactaccacc
     5761 aagaataata cccgcttcga ggtgaccagt tacaagaccg attaaagtac aagcaacaag
     5821 aggttgatgg atttggaatt ggtcgaggat accttcaaga ccagcaaaga aggcaacgat
     5881 aacgaccaaa atcattgaga taattgaaat atccgccatt ttgatatcct ttcgttttca
     5941 gagataataa gttattttac gtttgctttg ttgattaaat caaacaaatt ctttttagcg
     6001 tcatttggta ctttacgtac atcaaatgat acacctaagt cacgtaattt ttcaaaggca
     6061 gcaacatcgt ctttatccat tgaaagaacg ttgtttacca ttgttttacc agttgagtga
     6121 gccattgaac caacgttcaa ttcagggatt tccacaccac cttcaattgc acggagtgca
     6181 tcttgtacag tttcaaataa gatcaaggca cgtgtgtttc cgaaacgtgg gtctttagct
     6241 acttcaatta atttactaat tgggacaacg tttgctttga ctcctccagg agcagcttgc
     6301 ttaatcaatt gtttacgaag ttcatcttta gaaacttcat cagaagcaac gataatacgg
     6361 tttgcttttg atgctggagt ccatgcagtt gcaacttgac catgtaaaag acgtgtgtca
     6421 atacgagcaa gattaatttt aagcttgcca tctccaataa cagttccttc agggatagat
     6481 ccactttgtt taacttccgc aggaacatct gcaggaacac ctgctacagg agtagcttct
     6541 acaacaggat taagttcttc tggaagggct ttgatacctt ccttagactc tttaataata
     6601 tttgcggcaa cttgttcaac acctgcatta gcgtccatca tacgctcagt ataggcttgg
     6661 ataagcattg gcaaattgag accagtgatg atagccattt tacgttcagg attttctccc
     6721 ataacacggc ttgcttggtt aaatggtgac ccactccaaa ggtcagctaa cacaagcacc
     6781 tcatcatcag catcaaattg tgcgatagca ttgttgaagt gtccatacaa atcatctggt
     6841 ccttcatttg gcatgaaagt tacaacttga actttttctt gttcaccaaa gatcattgaa
     6901 cctgattgat gaataccttc agcaaattta ccatggctgg caataataat accgataccc
     6961 attcttgttc ttcctcctta aaaaattttt tcgttgacat aaagaagaat aatctccttc
     7021 caatcgtaaa attggccatt ctttttcatt tcaacgaaat tatagaacag atatatttta
     7081 aaacgttttc agaaaaaaat caatactatt tctaataaaa attaaaaaaa aagatatttt
     7141 tttcacaaat accttttata ataggggaag agagtggtaa ctttttttgt taataatata
     7201 gtagaaaacc taaaaaacga tttctattag aattctgttt caatagagat catttttgaa
     7261 aatactacaa taataatttt tctagagttt tagcaacacc atcttcttcg tttgtccatt
     7321 ggatttgttg gtcggcatat ggaagtagag ttggattagc atttttcata gcatatcctg
     7381 tatgtgcaaa ggccagcatc tcggtgtcat tgtgttcatc tccaaaggca atcaaatttt
     7441 cttgtgaaag attgagtgat tttaataaat gtttgagagc ataagctttg ttaacacctt
     7501 taggtgaaaa ttcgagtata ttcaatggac caccccaaga atctacttct agttcgtgat
     7561 taaaatgttg tcgtaactct ttagctaact catacttatc tttatgacgt gtttgcatca
     7621 atagagcatt tggattgcgt gtgattttag taacatctaa tgccatttta tcagtaattt
     7681 cattgacacc gaataattgt gggttaattt tatcacgatt atccatagta atgtaaaaat
     7741 ttttacggta ttcgctagca ataaaatcgg cttggatagc atctgataaa tttaaggttt
     7801 caaggagtaa ttttttatca atcgttgcac tacgctcaaa cgcccacttt ttttcgggga
     7861 tatgagttaa agcaccatta aaatttatca ttggtgtttt taaatctagg cgtaaataat
     7921 gagcgagtgc catacggtat gggcgtccgg ttgtaataat tactttgtgt ccttgagctt
     7981 gaacttttcg aagtgtatca gcggtgtagt cagaaatagt gttattatta tgtaatagag
     8041 taccgtctaa atctacggcg ataattttct ttgtcatgct gacattatat caaaaataga
     8101 tttcaataaa aagagacagt agaaatatga tataataact gtggaaatat ggaagttggt
     8161 ggattatgaa aaagttaatt tggagtcatc tttttgccat agtcagtatt ttagggatat
     8221 ggtattatca tgtaaaaatt gatcatttga tggtaattct attggcatca gtcttgtcgt
     8281 tagagatggg attcattgct tattatagtc gtaatatttt tattcgtctt ttagcaagtt
     8341 taggtctttt atttttttac ccacagagtt tagggatttt tcttagtttg gtgagcttag
     8401 agtggtcaac aaaagacatt tggcagtcta atcactttat gcaatatatg gcataccttc
     8461 ttgcaattct ttttattatc tcttcagctt tactaagctt gaagctattg gtaaatgcat
     8521 atgatattag taaaaggtat caattagcaa ctgttatacc tgtaatgctt tttagtagtt
     8581 ttgccttttg cttagaaaaa tcaggatcat taggtttaga aggaatcatt ttaaatccgt
     8641 cagcattttt taatggaata cagactaata tggctaacct tgattttaaa gccttgtctt
     8701 gccttttttt agtacaatta ttactttggg gaatattaat ggaagactaa ccacaacagg
     8761 gttagtcttt taggtttgaa gtttatttaa ccttaattta ataatatgtt gtataatgag
     8821 atattaattg gaggttagaa tgaaagtaaa actatttgcg atacatctat tttttattgt
     8881 tatttctttt gggattaaat attatcattt gcagggacca aatttgattt ggaatatgac
     8941 tctagcccta atagcgttag attttgcata ccttactagt ttatttaaga aaaaaattct
     9001 tatcggtctt tttgcacttg cttggttttt tttctatcct aatacctttt atatgttgac
     9061 ggatatcatt catatgcatt ttgtcggaga cgttctttat aataagacaa atttaatact
     9121 ttatatcttg tatgtatcta gtatcttgtt tggtttttta tcaggtatcg aaagtttttc
     9181 tgtgattatg cgtaaattta gaattagcaa tatattttta agatggggaa ttattggaat
     9241 tgtatcattt gtttcaagtt ttggtattca tattggtcgt tatgcccgtc taaattcatg
     9301 ggatatctta acgaagccac aagtggtaat taacgaactt ttagcggttc cgagtaggga
     9361 ttcattccac tttattttag gatttacatt tttacaggta ttgtgtctta tttttatgga
     9421 ttattctgca gggaaaaagc ttgaaaagtg aattattttt tgttaatata tattagtcgt
     9481 tcgtaaatat aagaacgatt aattttttta aaagtgtaaa atagcaaaaa ataaaggaga
     9541 atccgatttt aaaatggaaa agttttttaa gctaaaggaa cacgggacga caattcggac
     9601 tgaaatcact gctggtctaa cgactttctt tgcaatgtcg tacattttgt ttgtaaatcc
     9661 agctatttta tctcagactg ggatgccagc acaaggagtt ttcttagcga cgattattgg
     9721 tgccgttgta gctacatctg ttatggcttt ctatgctaac cttccctatg cacaagctcc
     9781 tgggatggga ttaaatgctt tctttacata tacagttgtc tttgcattgg gatatacatg
     9841 gcaagaagcc ttagctatgg tatttatttg tggtttgatt tctctgatta ttactttaac
     9901 gaaagttaga aagatgatta ttgagtctat cccaacaact ttaaaatcag ccattacagc
     9961 aggaatcgga acttttttag catacgttgg gattaaaaat gctggttttt tgaaattttc
    10021 aattgatcct gggacgtatg atgttgttgg aaagggtgct gcgaaaggat tggctactat
    10081 tacagcaaat tcttcagcaa cgccagggct agtgtcgttt gataatccag ccattttatt
    10141 atctttgatt ggtttaagca ttaccatctt ctttattgta aagggtatcc gtggagggat
    10201 tattctatcg atattaacaa cgaccctctt aggtatttta atgggagtgg ttaagttaga
    10261 tgctattaat tgggaagcaa ctaacctctc ggcctctttt agagatttga agcaagtatt
    10321 tggagttgca ttaggagaga aaggattaat ttctttattt tctaatcctt cacgtctacc
    10381 aagtgtatta atggccattc ttgctttttc actgactgat atttttgata ccattggaac
    10441 gctcattggt accggagaaa aagttggtat cttagcaacg actggtgata atcatgaatc
    10501 taagagttta gacaaagcac tctattcaga cttaattggg acaacttttg gtgctatttg
    10561 tggaacatct aacgttacaa cctatgttga gtcagcagca ggaatcggag ctggtggtcg
    10621 tacaggattg acagccttag tagttgctgg tttgtttgct atatctagtt tttttagtcc
    10681 actagtatct atcgttccga gtcaagctac tgcaccaata ttggttattg ttggtattat
    10741 gatgctttca aatttaaaag atatcaaatg ggatgatatg tcagaagcta tcccagcttt
    10801 ctttacttct ctttttatgg ggtttactta tagcattact tatggtattg cagctggttt
    10861 cttgacctat acactagcta aagtcatcaa aggacaagca aaagatattc atgtggtact
    10921 gtggatttta gatatattat ttattctaaa ctttatcagc ttggctattt tgtaatatta
    10981 tagtttgata aataagataa aaggaaatca gatttgtagc ctggtttcct tttttcttaa
    11041 gaaaagtgaa gaaatatatg ctatggggct gtcagaatca gcaaactgtt ggaagacaga
    11101 gatatttctg gtataatatc tctatgtttt atactcaaaa tgaagaagag cttattgccc
    11161 tagggcaaaa actgggtact gttttaaagt caggagatat cgtattattg actggtaatt
    11221 taggtgcagg caaaacaact cttacaaaag gtattgcaaa gggattagac ataaaacaaa
    11281 tgattaagag cccaacttac actattgtta gagaatatga agggcgtgta ccactttacc
    11341 atttggatgt ttatcgtatt ggagatgatc ctgattctat tgacttagat gacttcttgt
    11401 ttggtcaagg agtaacagtt attgaatggg gagagttgct tagtgataac cttatcaata
    11461 actacttaga aattgttatt acgaggagca atcaaggacg tcaggttcag ttagaagctt
    11521 atgggcatcg cgcaagagaa attattgagg cgattcaaga tgtctaaaca atttgctgtt
    11581 gattttatag aagcttcgag agaggaagca tctgcgatcc ttgaattttt aaatactgtt
    11641 actgaggaaa cagattttat tttgcatacg gtgagcaatc aactatcttt gtcagaaatg
    11701 gaaacgttta ttgaaaatac cttaatgacg aaaaattgca tttgtttgat tgctaaatta
    11761 aaaaataaag tgatcggctt gataacaatc attagtcagt ctgatattga aattgaacac
    11821 gttggggatc tttttattgc cgttcagaag gattattggg gatatggtat tggtcatatt
    11881 ctcatggaag aagcaataga atgggcgtca gataacgata ttacacgccg cttagaacta
    11941 tcagtccaag gacgtaatga acgtgctatt cacctctacc aaaaattcgg ttttgaaata
    12001 gatgggcttc aaataagagg tattaaaaga gaaaatggag aatttctaga catttacaga
    12061 atgagtaagt tgatagatta ggaatataca tgaaaatttg gaaaaaaata accttaatgt
    12121 tttctgcaat tattttaaca acagtaattg cattgggagt ctatgttgcc tcagcttata
    12181 atttttcgac taatgaattg tctaagactt ttaaagattt taagctagct aaatcaaaaa
    12241 gtcatgctat tgaagaaaca aagccgtttt caatactatt aatgggggtg gacacaggtt
    12301 cagagcatcg aaaatctaag tggtcaggaa atagcgattc tatgatctta gtcactataa
    12361 atcctaaaac taataaaaca acgatgacaa gcttagaacg tgacgtattg attaaattga
    12421 gtggtcccaa aaataatgga cagactggag tagaagcaaa gctaaatgca gcctatgctt
    12481 ctggtggtgc ggaaatggca ttgatgactg ttcaagactt attagatatt aatgttgatt
    12541 actttatgca aattaatatg caaggattag ttgatttagt caatgctgtt ggtggtataa
    12601 cagtaactaa taaatttgac tttccaatat caattgctgc caatgaacca gagtacaagg
    12661 ctgttgttga accagggaca cataaaataa atggagaaca agcacttgtt tattctcgta
    12721 tgcgctatga tgatccagag ggagattatg ggcgtcaaaa aagacaacgt gaagtaattc
    12781 aaaaagtcct taaaaaaata ttggcgttaa atagtattag ttcatacaaa aaaattcttt
    12841 ccgcagtaag taataacatg caaactaata ttgagatatc atcaaaaacg attcctaatt
    12901 tgttagctta taaagattca ttggaacata ttaaatctta tcagttgaag ggtgaagacg
    12961 ctactttatc agatggtggc tcttatcaaa ttttaactaa gaaacatcta cttgcagttc
    13021 aaaatagaat taagaaagaa ctggataaaa agcgtagtaa aactctgaag acaagcgcga
    13081 ttctatatga agattactat ggtactactg ctagtaatga ttcttctact tattcatcaa
    13141 cacaagagaa taattataat acaacacctt attcagaagc accaccaagt tacagtggta
    13201 atactactta tagttctgag actaatcaaa caactcatca aaattactat aatagtagca
    13261 ctcctgctag taactatagc agtaacacta acacaggtca ggctgattca agtggaagtg
    13321 tcaataatca taacggggct gcaacgccta atccaaacac aggaacgcaa ccagtaccag
    13381 gtcaaactaa tccataaaat ataaaaaaga gcattatttg ctctttttat atttagagta
    13441 gatattttta taccgtcggc ttattcgtct ttctcttgat atttagccat gcgttgatta
    13501 atttcttcta aacgaggttg aatatcttta ttaaacactt ggaatttatg attgagttct
    13561 tgagaaatta atctgatttt ttctttttcc tgactaataa tagcaagatc atttttgata
    13621 cgttctaatt ttaatttagc tgaatctgtc tcattttttg tattttgata ggaatccttg
    13681 atagtttttc tatttttgta aagttcaagc gctcctaaac taactaaacc tagtgcaatt
    13741 aaacgaccta ttttcataat tgaatctctt ttctaatttc tttggctaat tttgcaagtg
    13801 cctcaaagtc gggttcatga gtagtgtagt gaattttgat accgtctgat tcatcaaagc
    13861 ggggaacaag gtggacgtgt gcatgaaaaa cagtttgtcc agcaatttcc tcattgttgt
    13921 taataatatt cattcctttg gcttttgtag cagcttggac agcacgcgcc actttaggca
    13981 agcgttcaaa ggttatctgt gcagtttttt catccatctc taacacattt cgaacatgtt
    14041 ttttaggaat aagaagggtg tgcccggtcg tagtttgtgt aatgtcgaga aaagctaata
    14101 cttcatcatc ttcataaact tttgatgaag ggatttctcc actgatgatt ttacaaaaaa
    14161 tacaattatc cataactaat tcctttcatg ttgaaaaatt tattgggtag ctattaagga
    14221 atggtaaatt tttatcgttt gataggtcat aagtcgtaca gggaaatgaa agtagcccgt
    14281 attgattgtg atatctatat ttaccatcat acttatctta gcatgaccta aagggtattg
    14341 ca
//
LOCUS       DATRMA010000044        13987 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.044, whole genome shotgun sequence.
ACCESSION   DATRMA010000044 DATRMA010000000
VERSION     DATRMA010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13987
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.044"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            complement(<1..87)
                     /locus_tag="VBC28_001314"
     CDS             complement(<1..87)
                     /locus_tag="VBC28_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HEO6815297.1"
                     /translation="MNNEFINFDRISRENWKDLHQQSQALLTE"
     gene            193..783
                     /locus_tag="VBC28_001315"
     CDS             193..783
                     /locus_tag="VBC28_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEO6815298.1"
                     /translation="MANMYYTENPNVEHDIHELNVKLLGESFSFLTDAGVFSKRMIDY
                     GSQVLLNSLHFEKNKSLLDLGCGYGPLGISLAKVQGVKATMVDINTRALELAKKNATR
                     NGVVVEVFQSNIYENISKTFDYIISNPPIRAGKQVVHSIIEESICYLNTGGSLTIVIQ
                     KKQGAPSAKAKMLDTFGNCDILKKDKGYYILRSEKV"
     gene            1109..1498
                     /locus_tag="VBC28_001316"
     CDS             1109..1498
                     /locus_tag="VBC28_001316"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEO6815299.1"
                     /translation="MGNIELKKLAVKASENAYVPYSKFPVGAALRTAEGKIFTGCNVE
                     NISYGLANCAERTAIFKAVSEGYKDFSEIAIYGNTEEPISPCGACRQVMVEFFNKNAK
                     VTLIAKNGKTVETTVGELLPYSFVDLK"
     gene            1563..2612
                     /locus_tag="VBC28_001317"
     CDS             1563..2612
                     /locus_tag="VBC28_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019315854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HEO6815300.1"
                     /translation="MNKKIAGIGLASIAVLSLAACGHRGASKSGGKSDSLKVAMVTDT
                     GGVDDKSFNQSGWEGMQAWGKKNGLKKGAGFDYFQSASESDYATNLDTAVSSGYKLIF
                     GIGFSLHDAIDKAADNNKDVNYVIVDDVIKGKDNVASVVFADNESAYLAGIAAAKTTK
                     TKTVGFVGGMESEVITRFEKGFEAGVKSVDKSIKIKVDYAGSFGDAAKGKTIAAAQYA
                     SGADIVYQVAGGTGAGVFSEAKSRNESLKEADKVWVLGVDRDQAAEGKYTSKDGKASN
                     FVLASSIKEVGKSVELIATKTSKGKFPGGNVTTYGLKDGGVDIATTNLSDDAVKAIKE
                     AKAKIISGDIKVPSK"
     gene            2757..4292
                     /locus_tag="VBC28_001318"
     CDS             2757..4292
                     /locus_tag="VBC28_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6815301.1"
                     /translation="MTQNVIEMKEITKKFGDFVANDHINLTVEKGEIHALLGENGAGK
                     STLMNMLAGLLEPTDGQIFINGQPVTIDSPSKSSQLGIGMVHQHFMLVEAFTVAENIV
                     LGNETTQNGVLDIKTAAKEIKELSEKYGLSVNPNAKISDISVGAQQRVEILKTLYRGA
                     DILIFDEPTAVLTPSEIKELMTIMKSLVKEGKSIILITHKLDEIRAVADKVTVIRRGK
                     SIETVPVAGASSQQLAEMMVGRSVSFRTEKKEANPTDIILSVKDLVVEENRGGVLAVK
                     NLSLDVRAGEIVGIAGIDGNGQSELIQAITGLRKVTSGQIVIKGKDVTKFSSRQITEL
                     SVGHVPEDRHRDGLVLDMTMAENLALQTYYKEPLSHKGILNFAKIKEYARQLMTEFDV
                     RGAGEHVLARGFSGGNQQKAIIAREVDRDPDLLIVSQPTRGLDVGAIEYIHKRLIEER
                     DKGKAVLVVSFELDEILNLSDRIAVIHDGKIQGIVKPDQTNKQELGILMAGGKIEKEE
                     RDV"
     gene            4285..5346
                     /locus_tag="VBC28_001319"
     CDS             4285..5346
                     /locus_tag="VBC28_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6815302.1"
                     /translation="MSKKAQKIAVPLISVVLGIILGAIIMLIFGYDPLWGYEGLFQTA
                     FGSIKNIGEIFRAMGPLILIALGFSVASRAGFFNIGLPGQALSGWIAAGWFALSHPDM
                     PRPAMILCTIIIGIVAGGITGAIPGILRAYLGTSEVIVTIMMNYIVLYSGNAIVQRVF
                     PKSIMRTSDSSVYVSANASYQTDWLSSLTNNSRINIGIFIAIIAVVLVWFLLNKTTLG
                     FEIRSVGLNPNASEYAGMSAKRTIILSMIISGAFAGLGGVVEGLGTFENVFVQPSSLA
                     IGFDGMAVSLLAANSPIGILFAAFLFGVLSVGAPGMNIAGIPPELIKVVTASIIFFVG
                     VHYIIEYVIKPKKQMKGGK"
     gene            5348..6304
                     /locus_tag="VBC28_001320"
     CDS             5348..6304
                     /locus_tag="VBC28_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6815303.1"
                     /translation="MSLTTIFALLFSSMLIYATPLIFTSIGGTFSERGGIVNVGLEGI
                     MVIGAFSGVVFNLEFASVFGDATPWISVLVGGLVGLIFSVIHAVATVNFRADHIISGT
                     VLNLMAPSLAVFLIKVLYNKGQTDNIQESFGKFNFPILSDIPFVGDIFFKGTSLVGYI
                     AILFSFLAWFILYKTRFGLRLRSVGEHPQAADTLGINVYLMRYSGVLISGFLGGIGGA
                     VYAQSISVNFAATTILGPGFISLAAMIFGKWNPIGAMLASLFFGLSQSLAVIGSHLPL
                     LSNIPTVYLQIAPYVLTIIVLAAFFGQAVAPKADGINYIKTK"
     gene            6509..7879
                     /locus_tag="VBC28_001321"
     CDS             6509..7879
                     /locus_tag="VBC28_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEO6815304.1"
                     /translation="MSKIVVVGTNHAGTAAIKTMLSNYGEANEIVTFDQNSNISFLGC
                     GMALWIGEQIDGPEGLFYSDKEQLESMGAKVYMNSPVLNIDYDKKEVTALVDGKEHVE
                     SYEKLILATGSQPIIPPIKGVEIQEGSREFKATLENLQFVKLYQNSEEVIEKLAKPGI
                     NRVAVVGAGYIGVELAEAFQRIGKEVTLVDVADTCMGGYYDRDFTDMMSKNLEDHGIR
                     LAFGQAVQAVEGDGKVERLVTDKETFDVDMVILAVGFRPNTELGAGKLDTFRNGAWVV
                     DKKQETSVKDVYAIGDCATIWDNSRDDINYIALASNAVRTGIVAAHNACGTELEGAGV
                     QGSNGISIYGLNMVSTGLTLEKAKQAGYNAVETGFNDLQKPEFIKHNNHEVAIKIVYD
                     KDSRVILGCQMVSHEDVSMGIHMFSLAIQEKVTIEKLALTDIFFLPHFNKPYNYITMA
                     ALGAKD"
     gene            complement(8000..8989)
                     /locus_tag="VBC28_001322"
     CDS             complement(8000..8989)
                     /locus_tag="VBC28_001322"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019768616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEO6815305.1"
                     /translation="MTATKQHKKVILVGDGAVGSSYAFALVNQGIAQELGIIEIPALF
                     DKAVGDAEDLSHALAFTSPKKIYAATYADCADADLVVITAGAPQKPGETRLDLVGKNL
                     AINKSIVTQVVESGFNGIFLVAANPVDVLTYSTWKFSGFPKERVIGSGTSLDSARFRQ
                     ALADKIGVDARSVHAYIMGEHGDSEFAVWSHANVAGVQLEQWLQENRDIDEQGLVDLF
                     ISVRDAAYSIINKKGATYYGIAVALARITKAILDDENAVLPLSVYQEGQYGDVKDVFI
                     GQPAIVGAHGIVRPVNIPLNDAELQKMQASAEQLKDIIDEAWKNPEFQEASKN"
     gene            9228..11687
                     /gene="gyrA"
                     /locus_tag="VBC28_001323"
     CDS             9228..11687
                     /gene="gyrA"
                     /locus_tag="VBC28_001323"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEO6815306.1"
                     /translation="MQDKNLVDVNLTSEMKTSFIDYAMSVIVARALPDVRDGLKPVHR
                     RILYGMNELGVTPDKPHKKSARITGDVMGKYHPHGDSSIYEAMVRMAQWWSYRHMLVD
                     GHGNFGSMDGDGAAAQRYTEARMSKIALEMLRDINKNTVDFQDNYDGSEREPLVLPAR
                     FPNLLVNGATGIAVGMATNIPPHNLGESIDAVKLVMDNPDVTTRELMEVIPGPDFPTG
                     ALVMGRSGIHRAYETGKGSIVLRSRTEIETTSNGKERIVVTEFPYGVNKTKVHEHIVR
                     LAQEKRIEGITAVRDESSREGVRFVIEVRRAASANVILNNLFKLTSLQTNFSFNMLAI
                     EKGVPKILSLRQIIDNYIEHQKEVIVRRTQFDKAKAGARAHILEGLLVALDHLDEVIT
                     IIRNSETDTIAQAELMSRFELSERQSQAILDMRLRRLTGLERDKIQSEYNDLLALIAD
                     LADILAKPERVVTIIKEEMDEVKRKYADARRTELMIGEVLSLEDEDLIEEEDVLITLS
                     NKGYIKRLAQDEFRAQKRGGRGIQGTGVNNDDFVRELVSTSTHDTVLFFTNLGRVYRL
                     KAYEIPEYGRTAKGLPIVNLLKLDEGETIQTIINARKEDVANKYFFFTTQQGIVKRTS
                     VSEFSNIRQNGLRAINLKENDELINVLLIDENEDVIIGTRTGYSVRFKVNAVRNMGRT
                     ATGVRGVNLREGDKVVGASRIVNGQEVLIITEKGYGKRTEASEYPTKGRGGKGIKTAN
                     ITAKNGPLARLVTINGNEDIMVITDTGVIIRTNVANISQTGRSTMGVKVMRLDQEAKI
                     VTVALVEQEIEDKSNIEDTKE"
     gene            11694..12437
                     /locus_tag="VBC28_001324"
     CDS             11694..12437
                     /locus_tag="VBC28_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HEO6815307.1"
                     /translation="MRNKKKSHGFFNFVRWLLVVLLIIVGLALVFNKPIRNAFIAHQS
                     NHYQISRVSKKTIEKNKKSKTSYDFSSVKSISTESILSAQTKSHNLPVIGGIAIPDVE
                     INLPIFKGLGNTELSYGAGTMKENQIMGGPNNYALASHHVFGLTGSSKMLFSPLEHAK
                     KGMKVYLTDKSKVYTYTITEISKVTPEHVEVIDDTPGKSQLTLVTCTDPEATERIIVH
                     AELEKTGEFSTADESILKAFSKKYNQINL"
     gene            12453..12866
                     /locus_tag="VBC28_001325"
     CDS             12453..12866
                     /locus_tag="VBC28_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEO6815308.1"
                     /translation="MKLKAVHHIAIIVSDYEKSKDFYVNKLGFEIIRENHRPERHDYK
                     LDLRCGDIELEIFGNRLDDPEYETPPQRIGRPNWPREACGLRHLAFYVPDVEAYKVEL
                     ENLGIFVEPIRYDDYTGKKMTFFFDPDGLPLELHE"
     gene            13019..13981
                     /locus_tag="VBC28_001326"
     CDS             13019..13981
                     /locus_tag="VBC28_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017766755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HEO6815309.1"
                     /translation="MSIKKLLFAGLAFILFTLASPAYAASDVQKVIDETYVQPDYVLG
                     YSLNQEQRAQTLQLLNYDESRDTKVKTLNTSSYAKIMNIADDASIQLYSSVKIKKLGS
                     NDTLAVNIVTPENITKVTEDMYRNAAVTLGIEHATISVAAPIKVTGESALAGIYYSLE
                     KNGASVSSENKQLAQEELSTLSGINAENKGKEGYDADKLNVALTDIKSAVAKGGSDLS
                     KDDIRKIVEETLKNYHLDNAVTENQINLIVNFAVNLSQSNVIKNSDFTNTLNNLKDNI
                     VSKAGSKFKNINVNFNANKAVESGKGFLANIWQQIVNFFQQFGS"
BASE COUNT         4565 a         2130 c         2866 g         4426 t
ORIGIN      
        1 ttcagttagt aaagcttggg attgctgatg taagtccttc caattttctc tggaaatacg
       61 gtcgaaatta ataaattcat tattcatgtc aaactcctat atgaaagcta ttataacatt
      121 ttattgagtg attgaaaacc gtttcaaaat atggtaaaat aatatggcga aattggaggg
      181 ttagaattat ttatggctaa tatgtattat acagagaatc ctaatgttga acatgatatt
      241 catgagctaa atgtcaaatt attaggagaa tcattcagtt ttttaactga tgcaggagtt
      301 ttctctaaaa gaatgataga ttatggtagt caagtccttc taaatagttt acattttgaa
      361 aaaaataaat ccttgttaga ccttgggtgt ggatacggtc cgctgggaat ttctctagcc
      421 aaagtgcaag gagttaaagc aactatggtt gacattaata cgagagcatt ggaattagct
      481 aagaaaaatg caacacgcaa tggagtagta gttgaagtct ttcagtcaaa catttatgaa
      541 aatatttcta aaactttcga ctatattata agcaacccac ctattcgtgc tggaaagcaa
      601 gttgttcatt ctattatcga agaaagtatc tgttacctta atacaggcgg tagccttacc
      661 attgtcattc aaaagaaaca aggcgctcca agtgcaaaag ctaaaatgct agatactttt
      721 ggtaattgcg atattttaaa aaaagataag ggatattata tattgagaag tgaaaaagtc
      781 taaatgatat ccctaaagcg taaaaataat aaatagttcg gataataact atcgtattat
      841 tttttggaaa ggttttctct aaaaatgttt tctttaaatt ccgtataaga taaaaagaat
      901 tctgcttttt attctgaaaa ttataaaaaa ctacttacat tatctaaaaa aaatggtaaa
      961 atagattcag ctaaaatacc aagactaatg aaactacaag acttggttac tcaataataa
     1021 atacttctgt gtttgataag aagataataa acgtgttcta aaaattttgc cagtaaacat
     1081 tatgaagcga aaggtgcagc tactaaaaat gggaaatata gagttgaaaa aattggctgt
     1141 aaaagcgagt gaaaatgcat acgttcctta ctcgaaattt ccagtaggcg cagcattgcg
     1201 tacagcagaa ggaaaaatat ttacaggatg taacgttgaa aatatcagtt atggattagc
     1261 aaactgtgct gagcgtactg caatttttaa agctgtttca gaaggttata aagatttttc
     1321 ggaaattgct atttatggaa atactgaaga accaatttct ccttgcggtg catgccgtca
     1381 agtgatggtg gaatttttta ataagaatgc taaagttaca ttgattgcta aaaatggtaa
     1441 gacggtcgag acgacggtcg gtgagttgtt accatactcg tttgttgatt taaaataatt
     1501 taaatcagtt gctcttttaa gcatttagct taaatattaa gttttttagg agggttcatt
     1561 tcatgaacaa aaaaattgcc gggatcggct tggcttcgat tgcagtactt agtttagctg
     1621 catgtggaca tcgtggtgct tctaaatctg gtggtaaatc agatagcttg aaggttgcaa
     1681 tggtaacaga taccggtggt gttgatgata aatcatttaa ccaatctggt tgggaaggta
     1741 tgcaagcttg gggcaagaag aatggcctta aaaaaggagc tggttttgac tatttccaat
     1801 cggcaagtga atctgattat gcaactaact tagatacagc tgtgtctagt ggttataaat
     1861 tgattttcgg tattggattt tctcttcatg atgctattga taaagcagca gacaataaca
     1921 aagatgttaa ttacgtcatc gttgatgatg ttattaaagg gaaagataat gttgcaagtg
     1981 ttgtctttgc ggataatgaa tcagcttact tagcaggtat tgcagccgct aaaactacca
     2041 aaacaaaaac agttggcttt gtaggtggta tggaatctga ggttattacc cgttttgaaa
     2101 aaggttttga agcaggtgtc aaatcagttg ataaatcaat taaaattaaa gttgactatg
     2161 ctggttcatt cggtgatgct gctaagggta agacaattgc agccgcacaa tatgcttctg
     2221 gcgcagatat tgtttatcaa gtagctggtg gaactggtgc aggtgtattc agtgaagcaa
     2281 aatcacgcaa tgaatcttta aaagaagctg acaaagtttg ggtattaggt gtagaccgtg
     2341 accaagcagc agaaggtaag tatacttcta aagatggtaa ggcttctaac tttgttttgg
     2401 cttcatcaat taaagaagtt ggaaaatcag ttgaattgat tgctactaaa acaagtaaag
     2461 gtaaattccc aggtggtaat gtaacaactt atggtttgaa ggatggtgga gttgatatag
     2521 caactacaaa cctttcagat gacgccgtta aagcgattaa agaggctaaa gctaaaatta
     2581 tttcaggaga tatcaaagtt cctagcaaat aatatcttta agaactttca aaaaagcgat
     2641 tagaaatcgc ttttttgaag ctgagataaa cttatatctc agtaaaatcc atcagtcgtt
     2701 aacgattggt ggattttact gaactataaa tgaatttttc tgaaagggag aaccacatga
     2761 cacagaatgt cattgaaatg aaagaaatca ctaagaaatt cggtgatttt gtggcaaatg
     2821 atcatatcaa tttgacggtt gaaaaaggtg agatacatgc tctgcttggc gaaaacggtg
     2881 cagggaaatc aactttaatg aacatgttag caggtttact agaaccaaca gacggtcaaa
     2941 tttttattaa tggtcagcct gtaactattg attcaccttc caaatcgtca caattgggga
     3001 ttggtatggt acatcagcat ttcatgcttg ttgaagcatt tactgtagct gaaaatattg
     3061 ttctaggaaa tgaaaccacc caaaatggtg ttttagacat taagacagca gctaaagaaa
     3121 taaaggaact atcagagaaa tatggtttgt cagtaaatcc aaatgctaaa atatctgata
     3181 tttctgtagg agcacagcag cgtgttgaaa ttttgaagac actttatcgc ggtgcagata
     3241 ttttaatttt tgatgagccg acggcggtgc ttacaccatc agaaataaaa gaattgatga
     3301 caattatgaa gagccttgtt aaagaaggca aatccattat actaattacc cataaattgg
     3361 atgaaattcg ggcagttgct gataaagtta ctgtgattcg ccgtggtaaa agtattgaaa
     3421 cagtccctgt tgcaggcgca agttctcaac aactggcgga aatgatggtt ggacgatctg
     3481 tttccttcag aactgaaaaa aaggaagcta atccgactga cataatcctt tctgtaaagg
     3541 atttagttgt agaagaaaat agaggcggcg ttctagctgt taaaaatctg tcgttagatg
     3601 ttcgagcagg tgaaattgta ggtatagctg gtattgatgg taatggacaa agtgaattaa
     3661 ttcaagctat tacaggttta agaaaggtaa cttctggtca aattgtaatt aaaggtaaag
     3721 atgtcacaaa attttcaagt cgacaaatta cagaattaag tgtaggtcat gttccagaag
     3781 atcgtcatag agatggtttg gttctagata tgacgatggc agaaaattta gctttacaaa
     3841 cttattataa agagccattg agtcataaag gtatccttaa ttttgctaaa attaaagagt
     3901 atgctcgtca attaatgaca gaatttgatg tgcgtggtgc tggtgaacat gttttagcaa
     3961 gaggattttc tggtggtaac caacaaaaag caattattgc tcgtgaagtg gatcgcgatc
     4021 cagacctact cattgttagc caaccgactc gtggtctaga tgttggtgct atcgagtata
     4081 ttcataaacg tttgattgaa gagcgcgata aagggaaagc ggtcttagta gttagctttg
     4141 agttagatga aatcttaaac ttatcagatc gtattgcagt tattcatgat ggtaaaattc
     4201 aaggaattgt taaacccgac caaaccaaca agcaagaatt gggtattttg atggctggtg
     4261 gaaaaattga aaaggaggag agagatgtct aagaaggcgc aaaaaattgc agttcctctg
     4321 atttcagttg tactaggaat cattctagga gctattatta tgctgatatt tggttatgat
     4381 ccactttggg gttatgaggg attgttccaa acagcattcg gtagtataaa aaatattggt
     4441 gaaattttcc gtgcaatggg accattaatt ttaattgctc taggtttctc agttgccagt
     4501 cgtgcaggtt tctttaatat tggacttcca gggcaagcct tatcaggatg gattgccgca
     4561 ggttggttcg ctctttcaca cccagatatg ccacgtccag caatgattct ttgtactatt
     4621 attattggta tcgttgctgg gggaatcact ggagctattc caggtattct cagagcatac
     4681 cttggaacaa gtgaagttat tgtaactatt atgatgaact atattgttct ttattcagga
     4741 aatgctattg tacaacgtgt tttcccaaaa agcattatgc gtacaagtga ctcgagtgta
     4801 tatgtttctg ccaatgcatc ataccagaca gattggttat ctagtttaac gaataattca
     4861 cgtatcaata ttggtatctt cattgctatt attgcagttg tgttagtttg gttcctatta
     4921 aataaaacaa cgcttggatt tgaaattcgt tcagttggtt taaatccaaa tgctagtgag
     4981 tacgctggta tgtctgcaaa gcgtacaatt attctatcaa tgattatttc tggagctttt
     5041 gcaggacttg gtggtgttgt tgaaggacta ggtacatttg agaatgtatt tgtacagcca
     5101 tcctcgttag ctattggctt tgacggtatg gcagtaagtc tactcgcagc gaatagtcca
     5161 ataggtattt tatttgcagc atttctattt ggtgttctaa gtgttggtgc gcctggtatg
     5221 aacattgcag gtattccacc tgagttaatt aaagttgtaa cagcttcaat catcttcttc
     5281 gtaggtgtcc attacattat tgagtatgtt attaaaccta aaaaacaaat gaaaggtggt
     5341 aagtaaattg agtttaacaa cgatttttgc attgctattt tcatcaatgc taatttacgc
     5401 aacacctctt atctttacaa gtattggggg aaccttctct gaacgtggtg gtatcgtcaa
     5461 cgttggttta gaaggaatta tggtaattgg agctttctca ggcgttgtat ttaatttaga
     5521 gtttgcttct gtttttggtg atgcgactcc atggatttct gtactagttg gtggtttagt
     5581 tggtttaatt ttttctgtta tccatgcagt tgcgacagtt aattttcgtg cagatcatat
     5641 tatcagcgga accgttctta atttaatggc accttcatta gctgttttct tgattaaggt
     5701 tctttataat aaaggtcaaa ctgataatat tcaagaatca tttggaaaat ttaatttccc
     5761 tatactatca gatattcctt ttgttggtga tatcttcttt aaaggaacaa gcttagtcgg
     5821 ttatattgct attcttttct cattccttgc ttggtttatt ctatataaaa ctcgctttgg
     5881 attaagatta cgctcagttg gagaacatcc tcaagcagca gacaccttag gtattaatgt
     5941 ttatcttatg cgttactctg gtgtcttgat ttcaggtttc cttggtggaa taggaggagc
     6001 tgtatatgct caatctattt ctgttaactt tgcagcgaca actatattag gaccgggctt
     6061 tatctcctta gcggcgatga tttttggtaa gtggaatcca attggtgcaa tgcttgctag
     6121 tctattcttt ggtttatcgc aaagtttagc agttatcggt agccatttac cacttttgag
     6181 caatattcca acagtttatc tacaaattgc tccatatgtc ctcactatca ttgtattagc
     6241 agctttcttt ggacaagcag tagctcctaa ggcggatggt attaattata ttaaaacgaa
     6301 ataaataaaa agaaactagt ataagctagt ttctttttat tgtttgtgaa tttgtgaaaa
     6361 aatactttgt gaaaatttca cataaattat aatacaataa caataaaata taaaaaaata
     6421 gaaaattgag tcattattct agtaagagtt tgtgaataat gttataatgt gttttgttat
     6481 taattattta taaaggagaa ctcttaatat gagtaaaatc gttgtcgtag ggacaaacca
     6541 cgctggtaca gcagcaatta aaacaatgct tagtaattat ggtgaagcca atgaaattgt
     6601 aacatttgat caaaattcca atatctcttt cttaggatgt ggtatggctc tttggattgg
     6661 tgaacagatt gatggtccag aaggattgtt ctactctgat aaagagcagt tggaaagtat
     6721 gggagctaag gtttacatga actctccagt acttaatatt gactacgata aaaaagaagt
     6781 tacagcttta gtagatggaa aagaacatgt tgaaagttat gaaaaactta ttttagcaac
     6841 tggttcacaa cctatcattc caccaattaa aggtgttgaa attcaagaag gatctcgtga
     6901 atttaaagct actcttgaaa accttcaatt tgtgaaactt tatcaaaatt cagaagaagt
     6961 tattgaaaag ttagctaaac ctggtatcaa tcgtgttgct gttgttgggg ctgggtatat
     7021 tggtgtagaa ttagcagaag ctttccaacg tattggaaaa gaagtaacac ttgttgatgt
     7081 tgctgatact tgtatgggtg gttactatga tcgtgacttt actgatatga tgagtaaaaa
     7141 ccttgaagac catggtattc gtcttgcttt cggacaagct gttcaagcag ttgaaggtga
     7201 tggtaaagta gaacgtcttg ttactgataa agaaacattt gatgttgata tggtgatctt
     7261 agctgttgga ttccgtccaa atacagaact cggtgcaggt aaattagata cattccgtaa
     7321 tggcgcttgg gtagttgata agaagcaaga aacaagcgtg aaagatgttt atgctatagg
     7381 tgattgtgcg acaatttggg ataattctcg tgatgatatc aattatattg ctcttgcttc
     7441 aaacgccgtt cgtactggta ttgtagcagc tcacaatgca tgtggtacag agcttgaagg
     7501 tgctggtgtc caagggtcaa atggtatctc aatctatggc cttaacatgg tgtcaactgg
     7561 tttgacttta gaaaaagcaa aacaagctgg gtacaatgca gttgaaacag gctttaatga
     7621 ccttcaaaaa ccagaattta tcaaacacaa taatcatgaa gtagctatta agattgtcta
     7681 tgataaagat agccgagtta tccttggttg ccaaatggtc tcacacgaag atgtatctat
     7741 gggaattcat atgttctcac ttgctattca agaaaaagtt actattgaaa agcttgcttt
     7801 gaccgatatc ttcttcttac cacattttaa taagccatat aactatatta caatggctgc
     7861 attaggcgct aaagattaat ttaaaattaa aaaggttaag cgaaatgctt agccttttat
     7921 ttcatccttt aacaaggtca aaaatgtaac ggcaaagaaa aagctaagat attcctatcc
     7981 tagctttttt tcaactatgt tagttttttg atgcttcttg gaattctggg tttttccaag
     8041 cttcgtcaat aatatctttt aattgttcag ctgaggcttg cattttttga agttcagcat
     8101 catttaatgg gatattaact ggacgaacga taccatgtgc acctacgatt gcaggctgac
     8161 cgataaagac atctttaaca tcaccgtatt ggccttcttg atatacagat aatggaagaa
     8221 ctgcattttc atcatcaagg atagctttag taatacgagc aagtgcgaca gcaataccgt
     8281 aatatgtagc accttttttg ttgatgattg agtatgcagc gtcacgaact gaaataaaca
     8341 aatcaacaag tccttgttca tcaatatcgc gattttcttg taaccattgt tcaagttgga
     8401 caccagcaac gttagcatgt gaccaaacgg caaattctga gtcaccgtgt tcacccatga
     8461 tgtatgcgtg aactgaacga gcatcaacac caattttatc tgctaaagct tgacggaaac
     8521 gtgcagagtc aagtgaagta cctgaaccaa taacgcgttc cttagggaaa cctgagaatt
     8581 tccaagttga atatgtaagt acatctacag ggttagccgc tactaagaag ataccgttga
     8641 aacctgattc aacaacttga gttacgattg atttgttaat tgctaagttt ttaccaacaa
     8701 ggtcaagtcg agtttcacct ggtttttgag gtgcgccagc agtaatgaca acaaggtcag
     8761 catctgcgca gtctgcataa gtagctgcgt agattttttt aggtgatgta aatgcaaggg
     8821 catgtgaaag atcttcagca tcaccaacag ctttatcaaa taaagctggg atttcaatga
     8881 tacctaactc ttgcgcaata ccttggttaa caagtgcaaa agcgtaagaa gaacctacgg
     8941 caccatcacc aacgagaata acttttttat gttgtttagt tgcagtcatt tctaaacatc
     9001 tccttaaata tttttgggaa gtttcccata cacctatatt ctatcatttt caatgaaaga
     9061 agtcactaat aaaatgtgaa aaaatttgca aacaaattga aatatcatag aaaactattg
     9121 aaaacgctga gtgtcttgat atttttgata ccgatttatc atcgcaattt tacctaaatt
     9181 atggtacaat gtaagagact tacagtgaag aaaaggggca ttttttaatg caagataaaa
     9241 atttagtaga tgttaatcta actagtgaaa tgaaaacgag ttttatcgat tacgccatga
     9301 gtgtcattgt tgctcgtgca cttccagatg ttagagatgg tttaaaacct gttcatcgtc
     9361 gtattttata tggtatgaat gaactaggtg tgacacctga taaacctcat aagaaatcag
     9421 cacgtattac tggtgatgtt atgggtaaat accatccaca tggtgattca tctatttacg
     9481 aagcaatggt gcgtatggca caatggtggt catatcgtca tatgctcgtt gatggtcatg
     9541 gaaactttgg ttcaatggat ggggatggtg ctgccgcaca gcgttataca gaagcacgta
     9601 tgagtaaaat tgctcttgag atgcttcgtg atatcaataa aaatacagtt gattttcaag
     9661 ataactatga tggcagtgaa cgtgaacctc ttgttttacc agcgcgtttc cctaatttat
     9721 tagtcaatgg tgcaactggt attgctgtag gtatggcaac aaatattcca ccacataatc
     9781 taggtgagtc tatagatgct gtcaaattgg ttatggataa ccctgatgtt actacacggg
     9841 agttaatgga agttattcct ggtccagatt ttcctactgg tgccttggtg atgggacgtt
     9901 caggtattca ccgtgcctat gaaactggga agggatcaat tgtcttacgt tcacggactg
     9961 aaattgaaac aacttcaaat gggaaagagc gtattgttgt cacagagttt ccatatggcg
    10021 ttaataaaac aaaagtacac gagcatattg tacgtttagc acaagaaaag cgcattgaag
    10081 gtattactgc tgttcgtgat gaatcaagtc gtgaaggcgt tcgttttgtt attgaagttc
    10141 gtcgtgctgc ttcagcaaat gtgattttaa ataatctttt caaattaact agcttgcaga
    10201 caaattttag cttcaatatg cttgctattg aaaaaggtgt tccaaaaatt ttgtcattac
    10261 gacaaatcat tgataactat attgaacacc aaaaggaagt tattgttcgt cgtactcagt
    10321 ttgacaaagc taaagcagga gcccgtgctc atatcttaga aggtttactt gtggcgcttg
    10381 atcacttgga tgaagttatc acaattattc gtaatagtga gacagacacc attgctcaag
    10441 cagagttgat gtcacgtttt gaattatcgg agcgtcagag tcaagctatt ttagatatgc
    10501 gtcttcgtcg tttaactgga ttagaacgcg ataaaatcca atcagaatac aatgacttac
    10561 tagctttaat tgctgattta gcagatatct tagctaagcc tgaacgtgtt gttaccatta
    10621 taaaagaaga gatggatgag gttaagcgta agtatgctga tgcccgtcgt acagaattaa
    10681 tgattggtga agttttatca cttgaagatg aagaccttat tgaggaagaa gatgttctta
    10741 ttactctctc aaacaaggga tatatcaagc gtctcgctca agatgagttt agagcacaaa
    10801 aacgtggagg acgcggtatt caagggactg gagttaataa cgatgatttt gttcgtgaat
    10861 tggtttcaac aagtactcac gatactgtgc ttttctttac aaatttaggg cgtgtgtatc
    10921 gtttgaaagc atatgaaatt ccagaatatg gacgtacagc taaaggcctc cctatcgtta
    10981 accttttaaa acttgacgaa ggtgagacaa ttcaaacaat tattaacgct agaaaagaag
    11041 atgtcgctaa taaatatttc tttttcacaa ctcagcaagg tatagtgaaa cgtacaagtg
    11101 tttctgaatt tagtaacatt cgtcaaaatg gacttcgtgc tattaatttg aaagagaatg
    11161 acgagttgat taacgtcctc ttgattgacg aaaatgaaga tgttattatc gggacacgta
    11221 caggctactc tgttcgcttc aaagtgaacg ctgtacgtaa tatgggacgc acagcgactg
    11281 gggtacgagg cgttaatctt cgtgaaggtg ataaagttgt gggagcttct cgtatcgtta
    11341 atggacaaga agtactaatc atcactgaaa aaggttatgg caagagaaca gaagcttcgg
    11401 aatatcctac aaaaggccgt ggtggtaaag gaataaaaac cgctaatatt actgctaaaa
    11461 atgggccatt agcacgctta gtgacgatta atggaaatga ggacatcatg gtcattacag
    11521 atacaggtgt tattattcga accaatgttg ctaatatttc tcaaactggt cgttctacca
    11581 tgggtgttaa agtgatgaga cttgatcaag aagctaaaat cgtcactgtt gcgctagtgg
    11641 aacaagaaat tgaagataag tctaatatag aggatacaaa agaataggaa gttatgcgta
    11701 ataaaaaaaa atcacatggt ttctttaact ttgttagatg gttattggtt gtattgctaa
    11761 ttattgttgg gttggctttg gtgttcaata aaccaattcg caatgctttc atagctcatc
    11821 aatcaaatca ttatcaaatt tcacgagtta gtaaaaaaac aattgaaaaa aataagaaat
    11881 ctaaaacaag ttatgacttt tcatcagtca aatctattag tacagaatcg attttatctg
    11941 ctcaaacgaa atcacataat ttgcctgtaa taggagggat tgctatacca gatgtcgaaa
    12001 tcaatctccc aattttcaaa ggactgggca atacagagtt gagttatggt gcaggaacga
    12061 tgaaagaaaa ccagattatg ggaggcccaa ataactatgc cttagctagt caccatgtct
    12121 ttggtttgac aggttcttct aagatgctct tttcaccttt agaacatgct aagaaaggta
    12181 tgaaagttta cctcactgat aaaagcaaag tatacactta cactattact gaaatttcaa
    12241 aggtaacacc agaacatgtt gaggttattg atgatacacc tggcaagagc caactcactt
    12301 tagtgacctg tacggatcca gaagccacag aacgtattat tgtgcatgct gaattagaaa
    12361 aaacaggtga attttcaaca gcagatgagt cgatattaaa agcattttcg aaaaaatata
    12421 atcaaattaa tctctagaag gtagttagaa ttatgaaatt aaaggctgtt catcatattg
    12481 cgataattgt ttctgactat gaaaagtcaa aagacttcta tgttaataag ctaggatttg
    12541 aaattattcg tgaaaaccat cgtccggagc gtcatgatta taaattagat ttacggtgcg
    12601 gtgatattga gcttgaaata tttggaaatc gtcttgatga tccagaatat gaaactcctc
    12661 ctcagagaat aggtaggccg aattggccac gtgaagcatg tggtttacga caccttgcct
    12721 tttacgttcc agatgtggaa gcgtacaagg tagaactgga gaatttagga atttttgtag
    12781 agcctattcg ttacgatgac tatactggca aaaagatgac ctttttcttt gatccagatg
    12841 gtttaccttt agaattacat gaataataaa caaagttgcg tggatacgca actttgttta
    12901 atttactgaa taattttata ttcaagaagt tttttaaaga aataagtaag ttaatcatgg
    12961 tataatgtaa ggtatgaaaa gaattgaaaa ggaattattt tgaaatgagg atactaaaat
    13021 gagtattaaa aagctattgt ttgcaggcct tgcttttatt ttatttaccc tagcgagtcc
    13081 agcatatgct gcatcagatg ttcagaaagt tattgatgaa acttatgttc aacctgatta
    13141 tgtcttaggt tattcgctaa atcaagaaca gcgtgcacag acattacaac ttctgaatta
    13201 tgatgaatct agagacacga aagttaaaac gctaaatact agctcatatg ctaaaataat
    13261 gaatatagct gatgatgcaa gcatccaatt atattcatct gttaaaataa aaaaattagg
    13321 gtctaacgat accttagcag taaacattgt tacacctgag aatattacta aagttacaga
    13381 agatatgtat cgcaatgctg cagttacatt aggtattgag catgcaacga tatcagtggc
    13441 tgctccaata aaagtaactg gggagagtgc tcttgcaggt atctattatt cattagagaa
    13501 aaatggagca agtgtatcat cagaaaataa acaactagca caagaagagt taagtacttt
    13561 atctggtatt aatgctgaga ataagggtaa ggaaggttat gacgccgaca agcttaatgt
    13621 agctttaacg gatattaagt cagccgtagc taaaggagga tctgatcttt caaaagatga
    13681 tattcgaaaa attgttgagg aaacattgaa aaattatcat cttgataacg ctgtaacgga
    13741 aaatcaaata aatttaattg ttaattttgc agttaatctt tcacaaagta atgtcatcaa
    13801 aaatagtgat ttcacgaaca ccttgaataa tttgaaagac aacattgttt cgaaagcagg
    13861 atctaaattt aaaaatatca atgtcaattt taatgctaat aaagctgttg aatcaggtaa
    13921 gggattttta gctaatattt ggcaacaaat tgttaatttt ttccagcagt ttggtagtta
    13981 ataaaaa
//
LOCUS       DATRMA010000045        13965 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.045, whole genome shotgun sequence.
ACCESSION   DATRMA010000045 DATRMA010000000
VERSION     DATRMA010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13965
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.045"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            complement(<1..849)
                     /locus_tag="VBC28_001327"
     CDS             complement(<1..849)
                     /locus_tag="VBC28_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEO6815310.1"
                     /translation="MATSTENLQLAKRGPIISIIAYITLAVAKLAAGYWFDATSLVAD
                     GFNNLSDILGNVALLIGLHLASQPADSNHRFGHWKIEDLANLITSFIMFVVGIQVFIQ
                     TVTKIINNTDTNIDPLGAIVGAISALVMLGVYFYNKQLSQRVKSSALVAASKDNLSDA
                     VTSIGTSIAIIAASLNFPIIDRLAAIIITYFILKTAYDIFIESAFSLSDGFDDYQLKQ
                     YEKAILTIPKISAVKSQRGRTYGSNIYLDIVLEMNPDLSVFESHAITERVEKLLSDKF
                     SVYDIDI"
     gene            complement(913..2259)
                     /locus_tag="VBC28_001328"
     CDS             complement(913..2259)
                     /locus_tag="VBC28_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEO6815311.1"
                     /translation="MLTLFTHINSFVWGPPLLALLVGTGIYLSFRLGFIQLRQLSRAF
                     KLIFREDNGQGDISSYAALATALAATVGTGNIVGVATAIKSGGPGALFWMWVAAFFGM
                     ATKYAEGLLAIKYRTKDTNGEISGGPMYYIVNGMGQKWKPLAVFFSAAGILVALLGIG
                     TFTQVNAIASSLEHTFKISTRFTSLILAVIVLFIIFGGIKSISKVSEKIVPFMAISYI
                     LATLIIIAVNYNKIPHTFQLIFSGAFSGTAAIGGFSGAIVKEAIQKGIARGVFSNESG
                     LGSAPIAAAAAKTKEPVEQGLISMTGTFIDTIVICTLTGIAILVTGKWLEFDLQGAPL
                     TQASFNTVFGSLGSFALTFCLVLFAFTTILGWSYYGERCFEYLFGTKFINAYRIIFVI
                     MVGLGGFLQLDLIWVIADIVNGLMALPNLIALLVLSPIIVKETQKYFSETKNITKP"
     regulatory      complement(2416..2548)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            2856..4181
                     /locus_tag="VBC28_001329"
     CDS             2856..4181
                     /locus_tag="VBC28_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020480284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HEO6815312.1"
                     /translation="MTLNAKGESNQILVDKEVHSTNLLLDIDEKPELFQGLLLSFQHV
                     FAMFGATILVPLILGMPVSVALFASGCGTLIYQVATKFKVPVYLGSSFAYITAMALAM
                     KQMHGDISAAQTGILFVGLIYVVVATVIKFVGNSWVDKILPPIIIGPMIIVIGLGLAN
                     SAVTNAGFVAKGDWRKMLVAVVTFLIAAFINTKGKGFIKIIPFLFAIIGGYILSIILG
                     LVDLSPVEKAAWFELPKFYLPFKTGLFHSYKLYFGPEMLAILPISIVTIAENIGDHTV
                     LGQICGRNFLKKPGLNRLLIGDGLATAFSALIGGPAETTYGENTGVIGMTRIASVTVI
                     RNAAFIAIAFSFFGKFTALISTIPSAVLGGMAILLYGVIASNGLKVLIENRVNFAEVR
                     NLIIASSMLVLGLGGAVLDLGALTLSGTALSAIVGIILNLILPKESMKS"
     gene            4314..4700
                     /locus_tag="VBC28_001330"
     CDS             4314..4700
                     /locus_tag="VBC28_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEO6815313.1"
                     /translation="MKFNLEQVKVFENYEIENWEEGQVTLTTKVVDSSLNYYGNAHGG
                     YLFTLCDQVSGLVAISTGYEAVTLQSNINYLRAGRLDDLLTVIGTCVHNGRTTKVVDV
                     HIKNQKGEMVTTAIFTMFVTGERKEK"
     gene            4806..7085
                     /gene="pcrA"
                     /locus_tag="VBC28_001331"
     CDS             4806..7085
                     /gene="pcrA"
                     /locus_tag="VBC28_001331"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HEO6815314.1"
                     /translation="MNPLIIGMNDKQAEAVQTTDGPLLIMAGAGSGKTRVLTHRIAYL
                     IDEKYVNPWNILAITFTNKAAREMRERAIALNPATQDTLIATFHSMCVRILRREADYI
                     GYNRNFTIVDPGEQRTLMKRIIKQLNLDTKKWNERSILGTISNAKNDLLDEIAYEKQA
                     GDMYTQVIAKCYKAYQEELRRSEAMDFDDLIMMTLRLFDQNKDVLAYYQQRYQYIHVD
                     EYQDTNHAQYQLVKLLASRFKNICVVGDADQSIYGWRGADMQNILDFEKDYPQAKVVL
                     LEENYRSTKKILQAANNVINHNKNRRPKKLWTQNDEGEQIVYHRANNEQEEAVFVAST
                     IDNIVREQGKNFKDFAVLYRTNAQSRTIEEALLKSNIPYTMVGGTKFYSRKEIRDVIA
                     YLNILANTSDNISFERIVNEPKRGVGPGTLEKIRSFAYEQNMSLLDASSNVMMSPLKG
                     KAAQAVWDLANLILTLRSKLDSLTVTEITENLLDKTGYLEALQVQNTLESQARIENIE
                     EFLSVTKNFDDNPEITVEGETGLDRLSRFLNDLALIADTDDSATETAEVTLMTLHAAK
                     GLEFPVVFLIGMEEGVFPLSRAIEDADELEEERRLAYVGITRAEQILFLTNANTRTLF
                     GKTSYNRPTRFIREIDDELIQHQGLARPVNSSFGVKYSKEQPTQFGQGMSLQQALQAR
                     KSNSQPQVTAQLQALNANNSHETSWEIGDVATHKKWGDGTVLEVSGSGKTQELKINFP
                     GIGLKKLLASVAPISKKEN"
     gene            7497..7742
                     /locus_tag="VBC28_001332"
     CDS             7497..7742
                     /locus_tag="VBC28_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEO6815315.1"
                     /translation="MGLIWSLIVGAIIGAIAGAITNKGGSMGWIANILAGLVGSFVGQ
                     SLLGTWGPKLADMALIPSIVGAIIVIIVTSFVLGKKS"
     gene            7790..8038
                     /locus_tag="VBC28_001333"
     CDS             7790..8038
                     /locus_tag="VBC28_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEO6815316.1"
                     /translation="MSLIWSLIVGAIIGAIAGAVTNKGGSMGWIANILAGLVGSFVGQ
                     SLLGTWGPKLAGMALIPSIVGAIIVVIVTSFVLGKMNN"
     gene            8053..8634
                     /gene="amaP"
                     /locus_tag="VBC28_001334"
     CDS             8053..8634
                     /gene="amaP"
                     /locus_tag="VBC28_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HEO6815317.1"
                     /translation="MSKGLKSLYTLLGLISLTLLGFVAVISKQHIYLPSFNWLDWDFN
                     LPSPIDVGMYHYFFWGALVLFVIVLLAILVVLFYPRRYTEYKLADKTGKLMLKKSAIE
                     GFVKTEVLKTGLMKSLSVTAHLYKKKVKVDVKGLLTSRTNVPEQLEHIQSDVELGLKE
                     FFGLEKKMNTRVFVKQVEEENVGNAKTNKSRVE"
     gene            8644..8838
                     /locus_tag="VBC28_001335"
     CDS             8644..8838
                     /locus_tag="VBC28_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HEO6815318.1"
                     /translation="MSEFVRKYRYPLGGAVIGLVLAAMIVTIGFFKTILALVIIVLGA
                     YAGLYVQRTGMLDQFFNKRK"
     gene            8894..9436
                     /locus_tag="VBC28_001336"
     CDS             8894..9436
                     /locus_tag="VBC28_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEO6815319.1"
                     /translation="MTETYIKNTTNNSGTTAVRGELTFEDKVIEKIVGIAIEHVDGLL
                     AVNGGFFSNLKNSVVNSDSVTDGVNVEVGKKQVAVDLDIVAEYQKHVPTIFADIKKVV
                     EAEVKRMTDLEVVEVNVNVVDIKTRAQHEEDSVTLQDRVTSAAQATGEFASNQVSNVK
                     SAVGNGVDKVEDMKSEPRVQ"
     gene            9495..9692
                     /locus_tag="VBC28_001337"
     CDS             9495..9692
                     /locus_tag="VBC28_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEO6815320.1"
                     /translation="MSEEKFDAKVDKVSGSVKESVGKLTGDKEVESEGKVDKLKGHAK
                     EKLADIKDTIKGASESFKKKD"
     gene            9714..10265
                     /locus_tag="VBC28_001338"
     CDS             9714..10265
                     /locus_tag="VBC28_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEO6815321.1"
                     /translation="MENQKQDIKTSVTYEEKVIAKIVGHALESVDGLLAVSGGFFSNL
                     KNSVVNSDSVTDGVNVEVGTKEVAVDLDIVVEYGKDIPAIVESIKTIVSQNVEVMTHL
                     KVVELNANVVDVKTKAEHEADSVTVQDRVSDAAQATGNFASEQAGKAKAAISSGAEKT
                     KEAVSNGTEAAKEKISEARTSES"
     gene            10432..11073
                     /locus_tag="VBC28_001339"
     CDS             10432..11073
                     /locus_tag="VBC28_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000253259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEO6815322.1"
                     /translation="MVSEQSEIVTSKYQKIAVAVAQRIANGDYEVGEKLKSRTTIAST
                     FNVSPETARKGLNILADLQILTLKHGSGAIILSKEKAIAFLNQYETSHSVAILKGKIR
                     DNIKAQQQEMEELATLVDDFLLQTRAVSKQYPLAPYEIIVSEDSEHLGKSIGELNVWH
                     QTGATIVAIEHEGKFIVSPGPFSVIEQGDHIFFVGDEDVYARMKTYFNLRMGL"
     gene            11078..11842
                     /locus_tag="VBC28_001340"
     CDS             11078..11842
                     /locus_tag="VBC28_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000348145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HEO6815323.1"
                     /translation="MDFVFDIDGTLCFDGMSLSKEIQGILERAQSDYGHRVTFATARS
                     YRDTIGILGDKLSLSKIIGLNGATLHENGHLVDSYYLQSDFFSTIISYCHRHQIPYFV
                     DEVFNYATYQASKIPFIAYVDPQKRGELLEVSKIEKPIKMVLYFGDQLERADQMLAEL
                     NRFGLSSHFFHEFEKCLYINPIAVDKGKATKKLFGNRFIAFGNDKNDISMFDAAHYSV
                     QVGDFDELTPYANLRVSRESVHEGITMLFEKYKGIT"
     gene            11842..12501
                     /locus_tag="VBC28_001341"
     CDS             11842..12501
                     /locus_tag="VBC28_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000362921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HEO6815324.1"
                     /translation="MDLFLSLERKFKAASDKEVSKQQEAYLRHHFKCYGIKSPERRML
                     YKELIKAAKRQAKIDWQLLDKCWQSDYREYHHFVLDYLLAMSQFLTYNDCSRLEFYAR
                     HQQWWDSIDVLTKIFGNLSLKDDKVMNLLSEWSLDQDFWMRRLAIEHQLGFKEKTNTD
                     ILSLFILRNTGSQEFFINKAIGWALRDYSKYNKVWVKDFISNHYDELSTLSIREGSKY
                     L"
     gene            12574..13068
                     /gene="tpx"
                     /locus_tag="VBC28_001342"
     CDS             12574..13068
                     /gene="tpx"
                     /locus_tag="VBC28_001342"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HEO6815325.1"
                     /translation="MTTFLGNSVTFTGKQLQVGDIAKDFLLIATDLSQKSLKDFEGKK
                     KVISVVPSIDTGICSKQTRTFNEELSELDNTVVITVSMDLPFAQKRWCSAEGLDNVIL
                     LSDFYDHSFGQDYALLMNEWHLLTRAVLILDEHNKVTYTEYVDNVNSDVDYEAAINAA
                     KILP"
     gene            complement(13144..13677)
                     /locus_tag="VBC28_001343"
                     /pseudo
     CDS             complement(13144..13677)
                     /locus_tag="VBC28_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768380.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3278 domain-containing protein"
     gene            complement(13777..13950)
                     /locus_tag="VBC28_001344"
     CDS             complement(13777..13950)
                     /locus_tag="VBC28_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO6815326.1"
                     /translation="MKASISQLALAKSVGVARQTINLIENNKYNPSLDLCIRLAECLQ
                     TDLNTLFWDSRHL"
BASE COUNT         4628 a         2088 c         2745 g         4504 t
ORIGIN      
        1 aatatctatg tcatatacag aaaatttatc actgagtagt ttttccactc tttcggtaat
       61 ggcatgactt tcaaagactg agagatcagg gttcatttcg agaacaatat caaggtaaat
      121 attactaccg taagtacgtc cacgttgaga ttttacagcg gatattttag gaattgttaa
      181 tatagctttt tcgtattgtt ttaattgata atcatcgaaa ccatccgata ggctgaaagc
      241 agattctata aaaatatcat aagctgtttt aagaataaaa taagtaataa tgatagcagc
      301 taaacgatca ataataggaa aatttagaga agccgcaatg atagcaatag atgtgccgat
      361 agatgtcaca gcatcagaaa ggttatcttt agaagctgca accagagcgc tagactttac
      421 tctttgagat aattgtttat tatagaaata aacgccaagc ataacaagag cagatatagc
      481 acctactata gcacctagag gatcaatgtt agtatcagta ttattaataa tcttagtgac
      541 agtttgaata aaaacttgaa tacctacgac aaacataata aaagaggtga tcaggtttgc
      601 taaatcttca attttccagt gaccaaaacg atggtttgag tcggctggtt gactagccaa
      661 gtgtaaacca attaagagtg ctacatttcc taagatatca gataagttat taaatccatc
      721 tgctacaagt gaagtagcat caaaccaata acctgcagct aatttagcga ctgccagtgt
      781 tatataggca atgatactaa taataggccc tctttttgca agttgtaagt tttcagtaga
      841 agttgccatt ttcctaattt cctttcattt acacattata tcacttagga gcagactata
      901 aaactagtaa gttcaaggct tagtgatatt tttagtctct gaaaaatatt tctgtgtttc
      961 tttaacaata attggtgaca aaactaggag agctattaga tttggtaaag ccattaaacc
     1021 attaacgata tcagcgataa cccaaataag atcgagctgt aagaaaccac ctaagccaac
     1081 cataatgaca aaaataatac gataagcgtt aataaattta gtgccaaata gatattcaaa
     1141 gcaacgctct ccgtaatagc tccatcctaa aatagtagta aaagcaaata agacaaggca
     1201 gaaggttaga gcaaaactac ctagtgagcc aaatacggta ttaaaagatg cttgtgttaa
     1261 tggagcaccc tgtaaatcaa attctagcca ttttcctgta actaaaattg caatccctgt
     1321 caaagtacaa ataacaatag tgtcaatgaa ggttcctgtc attgaaataa gcccttgttc
     1381 aacaggttct tttgttttag ctgctgctgc agctattgga gcagaaccca atccagattc
     1441 atttgagaaa actcctctag caattccctt ttgaattgct tctttaacga tagctccaga
     1501 aaaaccacca atggctgctg tcccagaaaa agcaccgcta aagatgagtt ggaaagtatg
     1561 tggaatctta ttgtaattaa ctgcaataat aatcagagtt gctaaaatat agcttatagc
     1621 cataaaaggc actatttttt ctgaaacttt tgaaatagat tttatacctc caaatataat
     1681 aaataatact ataacagcaa gaatgaggct ggtaaatcta gttgatattt tgaaagtatg
     1741 ctcaagagaa gaagcaattg cattgacttg agtgaatgtc cctattccta aaagagctac
     1801 taagatacct gctgcagaaa aaaagactgc taatggtttc catttttgtc ccattccatt
     1861 gacaatgtag tacattggtc ctccagaaat ttctccatta gtatcttttg tgcgatattt
     1921 gatagctaat aatccttctg cgtattttgt tgccattcca aaaaaggcag ctacccacat
     1981 ccaaaacaaa gctcctggtc ctccagattt aatagccgta gccacaccaa cgatattacc
     2041 tgtccctacc gttgcagcaa gagcagttgc aagggcagca taacttgaaa tatctccttg
     2101 cccgttatcc tctcggaaaa tcaatttgaa agctctagaa agttgtctca attgaataaa
     2161 acctaagcga aatgataggt aaatacctgt tccgactaat aaagcaagta aaggtggacc
     2221 ccaaacgaag ctattgatat gagtaaaaag tgttagcata tgttctcctt tgatacatga
     2281 catacctatg gattctgtaa tgaaacgaaa aaagttatct ctaaaagaga taacttctga
     2341 aagatgctag ctaaaaagcg cacaaataaa ggtagtagta aacttaataa gcttactact
     2401 cattattaat attggctttt agcttctgtc cttttgcctg agagattgga ggagttgttt
     2461 tactcttctt gccccttcgg cgcctagagc acatcttggt gtcaatacaa agatgttgct
     2521 acttataggt ctctccagaa gtccgtctgt caatcagtcc tgtcttaaga cacctgagag
     2581 tgtaattcct tcggcgaaag tctaactttc ttctcctgaa tgacgtcatt cggtatattt
     2641 tattatgtta actatcataa tgaaaaagca tagttttgtc aatatagttt caaaaaagtc
     2701 ttgttttcta aaatgtctta tgctataatt ctttcagcgt aggtttttaa actcatcccg
     2761 tgaggtgaga aaaacatcag gaaaaaaata aagcctatga ctatattgct atagaaagtg
     2821 ggcttttttg tatgcccaag aggactagtt tagatatgac tttaaatgct aaaggtgaaa
     2881 gtaatcaaat tctagtagat aaggaagtac attctactaa cttattacta gatatcgatg
     2941 aaaaaccaga attatttcaa ggtttgttgt taagttttca acatgttttt gccatgtttg
     3001 gagctacgat attagttccg cttattttag ggatgcctgt atcagtggct ttatttgctt
     3061 ctggttgcgg aacgctcatt tatcaagttg cgacaaaatt taaagtccct gtttacctag
     3121 gctcatcatt tgcttatatt acagctatgg cattagccat gaaacaaatg catggcgata
     3181 tttcggctgc tcaaacaggt attctttttg taggtctaat ctatgtcgtt gtggcgacgg
     3241 ttattaagtt cgtaggaaat tcatgggttg ataagatttt gccaccaata atcattggac
     3301 ctatgattat tgttattggt ttaggtttgg caaactctgc tgtaactaat gcaggttttg
     3361 tcgctaaagg tgattggcgt aaaatgctag ttgctgttgt tacattcttg attgcagctt
     3421 ttatcaatac taaagggaaa ggttttatca agattattcc atttttattt gcaattattg
     3481 gaggttatat tttatcaatc attcttggct tggtagattt atctccagtt gaaaaagctg
     3541 cctggtttga attgccaaaa ttctatttac cgtttaagac aggtttattt cacagttata
     3601 aactttactt tggaccagaa atgcttgcaa ttcttccaat ctcaattgta acgattgctg
     3661 aaaatatcgg tgatcataca gttttaggtc agatttgtgg gcgtaacttt ctaaaaaaac
     3721 caggtttaaa tcgtctttta attggagatg gtttagcaac cgcattttct gctcttatag
     3781 gagggccagc tgaaactact tatggtgaaa atacaggtgt tattggcatg acgcgtatag
     3841 cttctgtaac ggttattcgt aatgcagctt ttattgcaat cgctttttca ttctttggta
     3901 aatttacagc tttgatctct acaattccta gtgcagtact tggtggaatg gctatattac
     3961 tttatggtgt tattgcaagt aatggattaa aagtgttgat tgaaaatcgt gtcaattttg
     4021 ctgaggttcg taatctgatt attgctagtt ccatgttagt tttaggcctc ggtggagcag
     4081 tccttgatct aggtgctcta actttatcag gaacagcatt aagtgctata gtgggtatta
     4141 tattaaattt aatattgcca aaagaaagca tgaaatcata acaaaagagc tcagagtatc
     4201 tgagctcttt tgttatgata gatcagttaa aaagtttaaa ataaaaacgt ttacagttaa
     4261 ggtttcctta ttttctgcta taatggtcat agaaaaagtg aggttatagt agaatgaaat
     4321 tcaatttaga acaagtaaaa gtatttgaga attacgaaat tgagaactgg gaagaaggtc
     4381 aagttacctt aacaacgaag gtcgttgact cttcgctcaa ctattatggt aatgctcatg
     4441 gtggttattt gtttacactg tgtgatcaag tcagtggttt agttgctatt tcaacaggat
     4501 atgaagcggt aactctacaa tcaaatatca actatttgag ggcaggccga ttagatgatc
     4561 tgcttactgt catcggtact tgtgttcata atggtcgtac tacaaaagtc gttgatgttc
     4621 acattaaaaa tcagaaaggc gaaatggtta caactgctat ttttacaatg tttgtaactg
     4681 gagagagaaa agagaagtaa aaaaggtagg agttagacag cgtctgttta actccttttt
     4741 ctattgggaa aaagatgaaa aaatgatata ctagagaggc aattttgaca aaattaggag
     4801 tagaaatgaa tcctttaata attggaatga atgataaaca agcagaagcg gtacaaacga
     4861 cagacggacc gcttttgatt atggcaggag ctggctctgg aaaaacacgt gttctgactc
     4921 atcgtattgc ttatttaata gatgaaaaat atgttaatcc ttggaatatt ttagcgatta
     4981 cttttactaa taaagcagcg cgtgaaatgc gtgaacgtgc tatcgccttg aatccagcta
     5041 cccaagatac tttgattgca acttttcata gtatgtgtgt tcgtatttta cgtcgtgaag
     5101 ctgattatat tggatataat cgtaacttta caatcgttga tccaggtgag cagcgtactt
     5161 taatgaagcg aatcattaag caattaaatt tggacacaaa aaagtggaat gaacgttcaa
     5221 ttttaggtac aatctctaat gctaaaaatg accttcttga tgaaattgca tatgagaagc
     5281 aagccggaga tatgtataca caggtaattg ctaaatgcta taaagcttat caagaggaat
     5341 tacgtagaag tgaggccatg gatttcgatg acttgattat gatgacactt cgattatttg
     5401 atcagaataa agatgttttg gcctattacc aacagaggta tcaatatatc catgtagacg
     5461 agtatcaaga tactaaccac gctcaatacc aattagttaa gttattagct tcgcgtttca
     5521 aaaatatttg tgttgttggt gatgccgatc aatccattta cggatggcgt ggagctgata
     5581 tgcaaaatat tcttgatttt gaaaaggact atccgcaagc caaagttgta ttattagaag
     5641 aaaattatcg atcgactaag aaaatacttc aagctgctaa taatgtgatt aatcataata
     5701 aaaatcgccg tcccaaaaaa ttatggactc aaaatgatga aggtgagcaa attgtatatc
     5761 atagagctaa caatgagcaa gaagaagccg tttttgtagc atcaactatt gataacatcg
     5821 ttcgagaaca aggaaaaaat ttcaaagatt ttgccgttct ttatcgtacg aatgcgcaat
     5881 ctcgtactat tgaggaagca cttttaaaat ccaatattcc atatacaatg gttggtggaa
     5941 caaaattcta tagtcgtaaa gaaattcgag atgttattgc ttatctcaat atccttgcaa
     6001 atacttctga taatatttct tttgagcgga ttgtaaatga acctaaaaga ggggttgggc
     6061 caggcacttt agaaaaaata cggtcatttg cctatgaaca gaacatgtct cttcttgatg
     6121 cctcttcaaa tgtcatgatg tcgcccttaa aagggaaggc tgctcaagct gtttgggatt
     6181 tagctaatct gattttgact ctacgtagta agctagatag tttaactgta acggagatta
     6241 cagaaaacct actggataag acaggatatc tagaagcact tcaagttcaa aatacattag
     6301 aaagtcaagc gcgtattgaa aatattgaag aattcttgtc agtgactaaa aattttgacg
     6361 ataaccctga gattacagta gagggtgaaa ctggtttaga tcgtctatcg cgttttttaa
     6421 atgatttagc tttaatagca gatactgatg atagcgccac tgaaacggct gaggtcactt
     6481 taatgacatt acatgctgcc aagggtctag aatttccagt tgttttctta atcggtatgg
     6541 aagaaggagt atttccttta tcgcgtgcaa tagaggatgc tgatgaatta gaagaagagc
     6601 gtcgtttagc ttatgtcggg attacgagag cagaacaaat acttttcctt accaatgcta
     6661 atacccgtac tttatttggt aaaactagtt ataatagacc aacccgtttt atacgtgaga
     6721 ttgatgatga gctgatacaa catcaagggt tggctcgacc agttaattct tcatttggtg
     6781 tgaaatattc aaaagaacag cctacacaat ttggtcaagg aatgagtctt caacaagcgc
     6841 ttcaggcacg taaaagtaat tcacaacctc aagtaactgc ccaactccag gctcttaacg
     6901 cgaacaatag ccatgagaca tcatgggaga ttggtgatgt tgcaactcat aaaaaatggg
     6961 gggacggtac ggtgcttgaa gtttcaggta gcggaaagac tcaagagtta aaaataaatt
     7021 ttccaggtat tgggttaaag aaattattag cgagtgtcgc tcctattagt aaaaaggaaa
     7081 actaactttc ttctattata tgcttaaata atcagagact aagaattgta ttcttagtct
     7141 ttttgcatca tttgaataga caaaataagt tatcatttgg tttttactta ctaaaaaggt
     7201 tgatattaaa gcgtttttga aaagtgacca tactatttta aagaaagaag cgtttctctt
     7261 gcattatttt ctaaaaagag tataataaca atatcaatta aatatcaagt ggtcacttat
     7321 aaataaaagc atttaccaag ctttttactt ggcagtcact ttataaagtt gtataatatt
     7381 agtatagtta acaacttaat taattgccga gtggtcactt aagaaactct tgttatttag
     7441 ttaagaacaa aataagtttg tacactgaga tgtacttagg aagaaaggta tttatcatgg
     7501 ggttaatttg gtcattaatc gttggtgcta ttattggtgc tattgcagga gcaatcacta
     7561 acaaaggtgg ttcaatgggt tggatagcta acatcttagc tggtttagtt ggttcatttg
     7621 ttggtcaaag tttactagga acatgggggc ctaagttagc agatatggct ttgattcctt
     7681 caatcgtagg tgcgattatc gttatcattg ttacttcatt tgtattaggc aaaaaatcat
     7741 aactacataa ctagtagaat gattagtcaa gaaagagaag gtatttatca tgagtttaat
     7801 ttggtcatta atcgtaggtg ctattatcgg tgctattgca ggggctgtta ctaacaaagg
     7861 tggttcaatg ggttggatag ctaacatctt agctggttta gttggttcat ttgttggtca
     7921 aagtttacta ggaacatggg ggcctaaatt agcaggtatg gctttgattc cttcaatcgt
     7981 aggtgcgatt attgttgtca ttgttacttc atttgtacta ggtaaaatga ataactagaa
     8041 aaggagttac ctatgtcaaa aggactaaaa agtttatata ctttgctagg tctaatctcc
     8101 cttaccctac taggatttgt agctgttatc agtaaacaac acatttatct accatctttt
     8161 aattggttag attgggattt taatttgcca tcaccgattg acgttggtat gtatcattat
     8221 ttcttttggg gagcattagt gctgtttgta atcgttttac tggctatatt ggtagttctg
     8281 ttttatccaa gacgatacac agagtataaa ctggctgata aaactggtaa attgatgtta
     8341 aaaaaatctg caatagaggg atttgttaaa acagaagtat taaaaactgg tttgatgaaa
     8401 tctcttagtg tgacagctca tttatataag aaaaaagtga aagttgatgt taaaggtctt
     8461 ttaacttctc gtacaaatgt accggaacaa cttgaacata ttcaaagtga tgttgaactt
     8521 ggcttaaaag aattctttgg gttggaaaag aaaatgaaca ctagagtatt tgttaagcaa
     8581 gttgaagaag aaaatgttgg aaatgctaag acgaataaaa gtcgtgtaga ataggagtct
     8641 aatatgtcag aattcgtgag gaaatacaga tatccactag gaggtgcagt tattgggctc
     8701 gttttagctg caatgattgt aaccattgga ttctttaaaa caattcttgc tcttgttatc
     8761 attgtgctag gtgcttatgc tgggctttat gtgcaaagaa caggtatgct tgaccaattc
     8821 tttaataaga gaaaataaaa tttttcgttt atatttattc tacattattt aaataaaaga
     8881 aagaggaact attatgactg aaacttacat caaaaacaca actaacaatt ctggcacgac
     8941 agctgttcgt ggagaattaa ctttcgaaga taaagtgatt gaaaaaattg ttggaattgc
     9001 gattgagcat gtagatggtc ttttggctgt aaatggtggt tttttctcaa atttaaaaaa
     9061 tagcgttgta aattctgatt cagttacaga tggcgtaaat gttgaagtgg gtaaaaagca
     9121 agttgctgtt gatttggata ttgttgctga ataccaaaaa catgttccaa ctatctttgc
     9181 cgatatcaaa aaagttgttg aagctgaagt gaaacgtatg acagaccttg aagtggttga
     9241 agttaatgtt aatgttgttg atattaaaac tcgtgctcaa catgaagaag attcagtaac
     9301 tcttcaagat cgtgtaacaa gtgcagcaca agcaacaggt gaatttgctt cgaatcaagt
     9361 ttcaaacgtt aaaagtgccg taggtaacgg tgttgataaa gtggaagaca tgaagtctga
     9421 accacgtgta caataattga tttaagtata gtttaaagta ttgtttaaag tattgttatt
     9481 agtaggaggc tattatgtct gaagaaaaat ttgatgcaaa agttgataag gtttctggtt
     9541 cagtaaaaga atctgttggg aaacttactg gcgataaaga agttgaatct gaaggtaaag
     9601 tcgataaatt gaaaggtcat gcaaaagaaa aacttgctga cattaaagat accattaagg
     9661 gtgcttcaga gagctttaag aaaaaggact agaaggtttg aggtcattta tttatggaaa
     9721 atcaaaaaca agatattaaa acttcagtaa cttatgaaga aaaggtaatt gctaaaattg
     9781 ttggacatgc tttagaatca gtagacggac tcttggctgt gagtggaggt ttcttctcta
     9841 atctaaaaaa tagcgttgta aattctgact cagtaactga tggtgtaaat gttgaagttg
     9901 gtactaaaga agttgcagtt gatttagaca tcgttgttga atacggcaag gatatcccag
     9961 ctatcgttga aagcattaaa acgattgtca gtcaaaacgt tgaagtgatg acccacctta
    10021 aagttgttga attgaacgca aatgtcgtag atgttaagac taaagctgaa catgaagctg
    10081 actcagtgac agtacaagat cgtgtatctg atgcagcaca agcaacagga aactttgctt
    10141 ctgaacaagc tggcaaagct aaagcagcaa tttcctctgg agcagagaaa acaaaagaag
    10201 cagtatcaaa tggtactgag gctgcaaaag aaaaaatctc agaagctcgt actagtgaat
    10261 cttaattcag taaactactt tttatgataa gaagacactc ggagctggga gaccagctcc
    10321 ttttatatga tatttactaa agacattaga tattaacaaa aaagatatat ttgcaatatt
    10381 ttgctaattg tggtttaata tgtatgtttt tattaggata aggagggttg gatggtttct
    10441 gaacaatctg aaattgtaac atctaaatat caaaaaatag cagtagcggt tgcacaaaga
    10501 atcgcaaatg gagattatga agttggagag aagttgaaat ctagaacaac tattgcttca
    10561 acattcaacg tatcaccaga aacagcacgt aagggtctta atattttagc agatttacag
    10621 attttaactt taaagcatgg cagtggagcc attattcttt ctaaagaaaa agcaattgca
    10681 ttcttaaacc aatatgaaac ctctcattca gttgctattt tgaaaggtaa aatccgtgat
    10741 aatattaagg ctcagcaaca agaaatggaa gagttagcta ctttagttga tgattttttg
    10801 cttcaaacac gtgcggttag taaacaatac ccactagcac catatgaaat tattgtcagt
    10861 gaagattctg aacaccttgg taagtcaata ggagaattaa atgtatggca tcaaactggt
    10921 gcgactattg tggctattga acacgaaggg aaatttattg tttctccggg gcctttctca
    10981 gttatagagc aaggagatca tatctttttt gttggtgatg aagatgttta tgcacgtatg
    11041 aaaacctatt ttaacttaag gatggggctt tgagagaatg gattttgtct ttgatataga
    11101 tggaactctt tgttttgatg gtatgagtct atctaaagaa atacaaggaa tactagagag
    11161 agcccagagt gattatggtc atagagtcac ttttgctacg gcgcgatctt atagagacac
    11221 tataggtatc cttggcgaca agctatcttt atcaaaaatt attggtttaa atggtgcgac
    11281 tttacatgaa aatggtcact tagtggatag ttactattta caaagtgact tttttagtac
    11341 tattatttca tattgtcatc gccatcagat tccatatttt gttgatgagg tctttaatta
    11401 cgctacttat caggcttcta aaattccctt tattgcttat gttgatcctc aaaaaagggg
    11461 agagcttcta gaagtttcta aaattgagaa accgataaaa atggtgttgt attttggtga
    11521 tcagctggaa agggcagacc aaatgttagc ggaattaaat cgttttggac ttagtagtca
    11581 ctttttccac gaatttgaaa aatgtttgta cataaatccc attgcagttg acaagggaaa
    11641 agccactaag aaattatttg ggaatcgttt tatagctttt ggcaatgata aaaatgatat
    11701 ttctatgttt gatgctgcgc attatagtgt tcaagtaggg gattttgatg aactgactcc
    11761 ttacgctaat ttacgtgtat caagagaatc tgtacatgaa ggaattacaa tgctttttga
    11821 aaaatacaag gggattacgt aatggattta ttcctaagtt tagagaggaa atttaaagct
    11881 gcaagtgaca aagaagtttc taagcaacag gaggcttatt tacgccatca ttttaagtgc
    11941 tatggtatta aatcacctga gcgacgtatg ctctacaaag agcttataaa ggccgctaaa
    12001 agacaagcaa aaattgattg gcaattgctt gataaatgtt ggcaaagtga ctatcgtgaa
    12061 tatcatcatt ttgtacttga ttatctatta gctatgagtc aatttctcac ctataatgat
    12121 tgctctagac tagagtttta cgctcgtcat caacagtggt gggatagtat tgatgttctg
    12181 actaagattt ttgggaactt atccttaaaa gatgataagg ttatgaattt actgtcagaa
    12241 tggtcacttg atcaagattt ttggatgagg cgactagcta ttgagcatca actaggattt
    12301 aaagaaaaaa caaatactga tattttgtca ctttttattt taagaaatac aggaagtcaa
    12361 gaatttttta tcaataaggc gattggctgg gcgcttaggg attattcaaa gtataataaa
    12421 gtatgggtaa aggactttat cagtaatcat tatgatgagt tgtcgacttt gagcatacgc
    12481 gaaggaagta agtaccttta attatcagtt caattattcg aaaaagaaga agcttcatag
    12541 taaaataaag gtaattgata aggaggtaaa cttatgacga catttcttgg aaattctgtt
    12601 acttttacag gaaaacagct acaagttggg gatattgcaa aagatttttt actaatagct
    12661 acagacttat ctcaaaaatc tttaaaagat tttgaaggga aaaagaaagt tattagcgtt
    12721 gttccttcta ttgatacagg tatttgttca aaacaaacac gtacttttaa tgaggaatta
    12781 tcagagctag ataacacggt ggttattact gtttctatgg atcttccatt tgctcagaag
    12841 cgttggtgca gcgctgaagg ccttgataac gtgattttat tatcagattt ttatgatcat
    12901 tcgtttggtc aagattacgc tcttttgatg aatgagtggc atttgttaac gcgtgcagtt
    12961 cttattttgg atgagcataa taaggtgact tacaccgaat acgttgacaa tgttaattca
    13021 gatgttgact acgaagcggc gattaatgct gcaaagatat taccataaat aataaggctg
    13081 gaaaattttc cagccttatt atttatatag acagtgagaa caattagaaa tgaaatgttg
    13141 tcattattca tctttttcaa tatcaacatt gtttttaaat attagataca taactccatt
    13201 gtaagctaca ccggacataa tactagacaa aataactttt aaagaaaata gtgatacaat
    13261 cagcggagtg ttattgttag ttgttaaacc caagataagc cactggatga cagtataaaa
    13321 tatcccgatt ttgataaatt tttgtttgtt tttctttatt gctactggat agtcattttt
    13381 tgagacttct agtggcgtta ttaaatcatg actgttgaga taagagggaa taacaattag
    13441 aaaaatacaa aaatatagaa agaatgttaa agctaggaga gtatatgcag tctctgtggg
    13501 atatttactt accgtgaact aggcgatgat agaggataaa atgtgataga gactagtggt
    13561 tgcaaataat agatttccaa ggtaattgat tttttgttct ctgtattcat cgaatgggcc
    13621 attaatacca tatagggatt ttattaattt tatccccaaa tttttctttt ctttcataat
    13681 attctcctat ccttgaacta tttaataaag tttgagattg aaataataac acgacctaac
    13741 ctcccgagtt ttcatagatt tagagagtct aatctgttat agatgtctac tatcccaaaa
    13801 aagagtattg aggtctgttt gtaggcattc cgctagtctt atacaaagat ctagcgaagg
    13861 attgtattta ttattttcaa taaggtttat tgtttgtctg gcgacaccaa ctgattttgc
    13921 taaagctaat tgagatatgc tagctttaat tctgtattct ctaag
//
LOCUS       DATRMA010000046        13479 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.046, whole genome shotgun sequence.
ACCESSION   DATRMA010000046 DATRMA010000000
VERSION     DATRMA010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13479
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.046"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            <1..561
                     /locus_tag="VBC28_001345"
     CDS             <1..561
                     /locus_tag="VBC28_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001106850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HEO6815327.1"
                     /translation="FKNICGKANEYDISKDKTYLFGYEESIGFCYGTFVRDKDAVSAS
                     MMVVEMTAYYKERGQTLLDVLQTIYDKFGYYNERQFSLELEGAEGQERISRIMEDFRQ
                     DPILQVGEMTLENSIDFKDGYKDFPKQNCLKYYFNEGSWYALRPSGTEPKIKCYLYTI
                     GCTEADSLSKLNAIESACRAKMNSTK"
     gene            700..1554
                     /locus_tag="VBC28_001346"
     CDS             700..1554
                     /locus_tag="VBC28_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HEO6815328.1"
                     /translation="MTELIDGKALSQKMQAELGRKVERLKEQHGIIPGLAVILVGDNP
                     ASQVYVRNKERSALEAGFKSETLRLSESISQEELIDIIHQYNEDKSIHGILVQLPLPQ
                     HINDKKIILAIDPKKDVDGFHPMNTGHLWSGRPMMVPCTPAGIMEMFREYHVDLEGKH
                     AVIIGRSNIVGKPMAQLLLDKNATVTLTHSRTRNLSEVTKEADILIVAIGQGHFVTKD
                     FVKEGAVVIDVGMNRDENGKLIGDVVFEQVAEVASMITPVPGGVGPMTITMLLEQTYQ
                     AALRSVSL"
     gene            1551..2387
                     /locus_tag="VBC28_001347"
     CDS             1551..2387
                     /locus_tag="VBC28_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000634980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEO6815329.1"
                     /translation="MIVGEQEARALIKPRPKSSHKGDYGSVLLIGGFYPYGGAIIMAA
                     LACVKTGAGLVTVATQSCNIPSLHSQLPEVMAFDSDDYKWLEKSIVQSDVIVIGPGLG
                     VSESSRKILNQTMEKIQSHQSVILDGSALTLLSEGAFPQTKAKNLVLTPHQKEWERLS
                     GIAVSQQTKENTQTALKSFPKGTILVAKSSHTRIFQDLDEKEIIVGGPYQATGGMGDT
                     LCGMIAGMLAQFKEASPLDKVSVGVYLHSAIAQGLSKEAYVVLPTTISDEIPKEMARL
                     SK"
     gene            2513..3853
                     /locus_tag="VBC28_001348"
     CDS             2513..3853
                     /locus_tag="VBC28_001348"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001286947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEO6815330.1"
                     /translation="MSDYLSVSTLTKYLKLKFDKDPYLERVYLTGQVSNFRRRPNHQY
                     FSLKDDKSVIQATMWSGHFKKLGFELEEGMKVNVVGRVQLYEPSGSYSIIVEKAEPDG
                     IGALAIQFEQLKKKLSQAGYFDDRHKQLIPQFVRKIGVVTSPSGAVIRDIITTVSRRF
                     PGVEILLFPTKVQGEGAAQEIAQTIALANEKKDLDLLIVGRGGGSIEDLWAFNEECVV
                     EAIFESRLPVISSVGHETDTTLADFVADRRAATPTAAAELATPVTKIDILSWITEREN
                     RMYQSSLRLIRTKEERLQKSKQSVIFRQPERLYDGFLQKLDNLNQQLTYSMRDKLQTV
                     RQKQGLLHQKLQGIDLKQRIHIYQERVVQSRRLLSSTMTSQYDSKLARFEKAQDALIS
                     LDSSRIVARGYAIIEKNHTLVSTTNGINEGDHLQVKMQDGLLEVEVKDVRQENI"
     gene            3831..4046
                     /locus_tag="VBC28_001349"
     CDS             3831..4046
                     /locus_tag="VBC28_001349"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEO6815331.1"
                     /translation="MSDKKTFEENLQELETIVSRLETGDVALEDAIAEFQKGMLISKE
                     LQRTLKEAEETLVKVMQADGTEVEMDT"
     gene            4046..4918
                     /locus_tag="VBC28_001350"
     CDS             4046..4918
                     /locus_tag="VBC28_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEO6815332.1"
                     /translation="MVTIEKIDEAIHRYYKQTHSVVSPDLIKAILYSVDGGGKRIRPR
                     ILLEILEGFGVELIDGHYDVAAALEMIHTGSLIHDDLPAMDNDDFRRGRLTNHKKFDE
                     ATAVLAGDSLFLDPFDLVVKAGFKADVTVRLIELLSMSAGSFGMVGGQMLDMKGENKV
                     LSIDDLSLIHINKTGRLLAYPFVAAGILAEKSEEVKGKLHQAGLLIGHAFQVRDDILD
                     VTASFEELGKTPNKDIVAEKTTYPNLLGLDKSQEILDDTLKKAQAIFQNLEKKANFNA
                     RKIIDIIEGLRLNG"
     gene            4911..5738
                     /locus_tag="VBC28_001351"
     CDS             4911..5738
                     /locus_tag="VBC28_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family rRNA
                     (cytidine-2'-O)-methyltransferase"
                     /protein_id="HEO6815333.1"
                     /translation="MAKERVDVLAYKQGLFDTREQAKRGVMAGMVINVINGERYDKPG
                     EKVADDTELKLKGEKLKYVSRGGLKLEKALQVFEISVADKLTIDIGASTGGFTDVMLQ
                     SGARLVYAVDVGTNQLVWKLRQDHRVRSMEQYNFRYAQKEDFKEGLPEFASIDVSFIS
                     LNLILPALKEILVDGGQVVALIKPQFEAGREQIGKNGIVKDKLVHEKVLTTVTNFTKD
                     YGYTVKHLDFSPIQGGHGNIEFLMHLQKCQDPQNLVLDQIQDVIEKAHKEFKKNEEE"
     gene            5725..6198
                     /locus_tag="VBC28_001352"
     CDS             5725..6198
                     /locus_tag="VBC28_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HEO6815334.1"
                     /translation="MKKSERLNLIKQIVLNHAVETQHELLRRLEAYGVTLTQATISRD
                     MNEIGIIKVPSAKGRYIYGLSNENDPIFTTAVAKPIKTSILSISDKLLGLEQFININV
                     IPGNSQLIKTFIMSHCQEHIFSLTADDNSLLLIAKSEADADHIRQSMIAMLEKKD"
     gene            6210..7868
                     /gene="recN"
                     /locus_tag="VBC28_001353"
     CDS             6210..7868
                     /gene="recN"
                     /locus_tag="VBC28_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000923612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEO6815335.1"
                     /translation="MLLEISIKNFAIIEEISLNFETGMTVLTGETGAGKSIIIDAMNM
                     MLGSRASVEVIRHGANKAEIEGFFSVEKNQSLVQLLEENGIELADELIIRREIFQNGR
                     SVSRINGQMVNLSTLKAVGHYLVDIHGQHDQEELMKPNMHILMLDEFGNTEFNVIKER
                     YQSLFDAYRQLRKRVLDKQKNEQENKSRIEMLEFQIAEIESVALKSDEDQTLLKQRDK
                     LMNHKNIADTLTNAYLMLDNEEFSSLSNVRSAMNDLMALEEFDREYKDLSTNLSEAYY
                     VIEEVTKRLGDVIDDLDFDAGLLQEIENRLDVINTITRKYGGDVNDVLDYFDNITKEY
                     SLLTGSEESSDALEKELKILEHDLIESANQLSLERHKLAKQLENEIKQELTELYMEKA
                     DFQVQFTKGKFNKEGNEIVEFYISTNPGEGFKPLVKVASGGELSRLMLAIKSAFSRKE
                     DKTSIVFDEVDTGVSGRVAQAIAQKIHKIGSHGQVLAISHLAQVIAIADYQYFIEKIS
                     SDSSTVSTVRLLSYEERVEEIAKMLAGNNVTDTARTQAKELLGS"
     gene            7981..8817
                     /locus_tag="VBC28_001354"
     CDS             7981..8817
                     /locus_tag="VBC28_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEO6815336.1"
                     /translation="MSKIKIVTDSSITIEPELIKELDITVVPLSVMIDGTLYSDNDLK
                     AQGEFLNLMRGSKELPKTSQPPVGVFAEIYEKLMNEGVEHIIAIHLTHTLSGTIEASR
                     QGANIAGADVTVIDSTFTDQCQKFQVVEAAKLAKEGADLDTILARVEEVRQKSELFIG
                     VSTLENLVKGGRIGRVTGLLSSLLNIKVIMELTNHELVPIVKGRGLKTFSKWLDNFVE
                     SAQTRKIAEIGISYCGKADMANNFREKLAVLGAPISVLETGSIIQTHTGEDAFAVMVR
                     YE"
     gene            8810..9649
                     /locus_tag="VBC28_001355"
     CDS             8810..9649
                     /locus_tag="VBC28_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEO6815337.1"
                     /translation="MNKKKILTGLSFFLVSLLLSFGIFSLIIPKSNPKLTKKDFLTKK
                     VIPLNYVALGDSLTEGVGDTTSQGGFVPLLSESLHNRYSYQVTSVNYGVSGNTSQQIL
                     KRMTTDPQIEKDLEKADLLTLTVGGNDVLAVIRKELSHLSLNSFEKPAEAYKERLKEI
                     LAKARQDNPKLPIYVLGIYNPFYLNFPQLTKMQTVIDNWNKATKEVVDASENVYFVPI
                     NDRLYKGINGKEGITESSNSQASITNDALFTGDHFHPNNIGYQIMSNAVMEKINETRK
                     NWP"
     gene            9624..10226
                     /locus_tag="VBC28_001356"
     CDS             9624..10226
                     /locus_tag="VBC28_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HEO6815338.1"
                     /translation="MKQEKTGRNLNFWKWAFLLLLAINLSFTAVIASRLIQVREPNTG
                     KISTGVQDKVKVGTFTTNKSQLNKTIALYLKQYQTKKMNYKIYAASSSILFEGSYQLL
                     GYEVPLYIYFEPYRLTNGAVQLKVTSFSVGTLPLPEKDVLQYIKSSYKLPNFVDIKPK
                     KSVININLQDLKNKEGIYLKATAIDLVNDNFSFDIFKKKP"
     gene            10329..10604
                     /locus_tag="VBC28_001357"
     CDS             10329..10604
                     /locus_tag="VBC28_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEO6815339.1"
                     /translation="MANKQDLIAKVAEATELTKKDSAAAVDAVFAAVADYLAEGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGAEIEIAASKVPAFKAGKALKDAVK"
     gene            complement(10692..11834)
                     /locus_tag="VBC28_001358"
     CDS             complement(10692..11834)
                     /locus_tag="VBC28_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEO6815340.1"
                     /translation="MWSEKHKSGKVNFVERYKDPYTNKWKRTSVLMEKDTPRIRKEAQ
                     RILDAKIADIVRKLQTSDMLFTNLIDEWWIFYQQEIKRSSIVTLKGNIREIRAEFGIN
                     IPVVNIDPRYVQNYLDNLDCSRNKKERNKSMLNLIFDYAVSLDIIKDNPARRAKLPKI
                     KKTLNDWKKIEEKYLEEEEIKRLLKELFRRPSTHRLGLLSEFMSLNGCRIGEAISIEP
                     DNIDFENKTLQLHGTYDRTNGYINGEKTSPKTLASYRETIMSKREMEILQEMEFINEL
                     EKNTNKRYRDMGYIFTTRNGVPIQINSFNLALKKANERLEQPINKNITSHIFRHTLVS
                     RLAENNVPLKAIMDRVGHADAKTTVQIYTHITKKMKSNIADIMENY"
     gene            complement(11952..12503)
                     /locus_tag="VBC28_001359"
     CDS             complement(11952..12503)
                     /locus_tag="VBC28_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015986700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6815341.1"
                     /translation="MDINKIKEFAVSTSEKAKDGIIKANEMRKRASQESKIVLPPTIN
                     LGFNTPTAIRKTVEGDYYIGFYSEKPELYEFVNLQFDGSRIIEKTVTKGKTTQKGRSG
                     SVLGGAAIGTVLALGVGTIIGGMAGGARKKKGTINTTATTTSEEIPGKAMITLRNIQT
                     GEVKTLKTKLTQAQYVNVNNFFK"
     gene            complement(12521..12907)
                     /locus_tag="VBC28_001360"
     CDS             complement(12521..12907)
                     /locus_tag="VBC28_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEO6815342.1"
                     /translation="MTIEEIVDCHKVNLAYFDNELWPRPGIYIDDIKVIFVNKSLSLD
                     AQKRVILHELGHRERNIGDYKTLREKHELEADRYMISHLIAEALSQLEDKREFNYLQF
                     MQYHQLTTTADECMVIDEYYNLLNVV"
     gene            complement(12911..13282)
                     /locus_tag="VBC28_001361"
     CDS             complement(12911..13282)
                     /locus_tag="VBC28_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO6815343.1"
                     /translation="MTEQTLNLIAFERIKNLADKQGVSLQTVSIAVGLSDNYIYNLRT
                     RKSSSSEAITKIADYFRVSTDYIYGRTDNPRIASDEVTGYSTEDLRKMAENAKTFDGK
                     PLTEDDINAIQNIIEIYLKGR"
BASE COUNT         4406 a         2108 c         2652 g         4313 t
ORIGIN      
        1 tttaaaaata tttgtggaaa agctaatgaa tatgatatct caaaggataa aacttatctc
       61 tttggctatg aagaaagtat tggtttttgc tatggcactt ttgtacgtga taaagatgct
      121 gtgagtgctt caatgatggt agtagaaatg actgcctatt ataaagaacg agggcaaaca
      181 cttttagacg ttttgcaaac catttacgat aaatttggct attacaacga gcgccaattt
      241 tctcttgagt tagagggtgc tgaggggcaa gaacgtatta gtcgtattat ggaggatttt
      301 agacaggacc caatattaca agtaggtgag atgacattgg agaattctat tgatttcaag
      361 gatggttata aggattttcc aaagcaaaat tgtttaaaat attattttaa tgagggttca
      421 tggtatgctt taaggccgtc agggacggaa cctaagataa aatgttacct ttatacgatt
      481 ggttgtacag aagcagatag tttatcgaaa cttaatgcaa ttgagtcggc ttgtcgtgct
      541 aaaatgaata gtactaaata agtcatatgt aaaaaggcga tgagggattc attgcctttt
      601 ctattgatta aaattgatac aaaatgaata gcgatataaa atagcataat cgctattttt
      661 tgatataata tagaaaaaac ggatgaagaa ggagtatcaa tgactgaatt aatagatggt
      721 aaagccttat cacaaaaaat gcaggctgag ttagggcgaa aagttgaaag gttaaaggaa
      781 cagcatggta tcatacctgg tttagctgta atccttgttg gggataatcc agctagccaa
      841 gtgtacgttc gaaataaaga acgctctgca ttagaagctg gttttaaaag tgagactcta
      901 cgattatctg aatccatttc tcaagaagaa ctgattgata ttatccatca atacaatgaa
      961 gataaaagta tccatggtat tttagttcaa cttccgttac cacaacatat taacgataaa
     1021 aaaattattt tagcaataga tcctaagaaa gatgttgatg gtttccaccc aatgaatacg
     1081 ggtcatcttt ggtcaggacg accgatgatg gtaccttgta cgccagctgg tattatggag
     1141 atgtttagag agtaccatgt ggatttagag ggaaaacatg ctgttatcat cggaaggtcc
     1201 aatattgttg gtaagccaat ggcacaactt ctccttgata aaaacgcaac agttactttg
     1261 acacattcaa gaactcgaaa cctttctgag gtaacaaagg aggctgatat ccttattgtt
     1321 gcgattggtc aggggcactt tgttacaaaa gacttcgtta aagaaggtgc tgtggtgatt
     1381 gatgttggta tgaatcgcga tgaaaatggt aaattgattg gagacgttgt atttgaacaa
     1441 gtggcagaag ttgctagtat gataacacct gttcctgggg gcgtaggacc tatgacgatt
     1501 acaatgcttt tggaacaaac ttatcaagca gctcttagaa gtgtgagtct atgattgttg
     1561 gtgaacaaga agcgagggcg ttaattaagc cacgccctaa atcaagccat aaaggtgatt
     1621 atggtagtgt tcttctgata ggaggttttt atccctatgg aggtgctatt ataatggcag
     1681 ctttggcctg tgtcaaaact ggtgcaggat tagttactgt agcaacccaa agttgcaata
     1741 tcccctcttt gcatagtcaa ctaccagagg taatggcgtt tgatagtgat gattacaaat
     1801 ggttggaaaa atcaattgtt caaagtgatg ttattgtaat tggtcctgga ttaggagtat
     1861 cagaatcatc tcgaaaaatt ttgaaccaga ccatggagaa gattcaatca catcaaagtg
     1921 tcatccttga cggatcagcc ttgactctgt tatcagaagg tgcgtttccg caaacaaagg
     1981 ctaaaaattt agtgttgaca cctcatcaaa aagaatggga gcgattgtca ggtatcgctg
     2041 tatcgcaaca gacaaaagaa aatacccaaa ccgctcttaa atcttttccc aaagggacga
     2101 ttttagtcgc taagagttcg catacgcgta tttttcaaga tttagacgaa aaagaaatta
     2161 tagtaggagg tccttaccag gcgactggag ggatggggga tactttgtgt ggtatgattg
     2221 caggtatgtt agcgcaattt aaagaagcct ctcctctaga taaggtatca gtgggagttt
     2281 atctacattc ggcaattgct caaggattat ctaaagaagc ctatgttgtt ctaccgacaa
     2341 cgattagtga tgagattcca aaagaaatgg ctagactatc taaataaaaa ataagtagag
     2401 aggtgttagc atgacacttc ttttgcttta tcgagctatt tgtagagcaa ataggtattt
     2461 tttaagtttt tttggtataa tgaagttaat aaaactctca tgaggaggtg ctatgtcaga
     2521 ctatttatcc gtgtcgacac tgactaagta tttaaaatta aaatttgaca aagaccctta
     2581 tttagagagg gtttatttaa ctgggcaagt gtctaatttt cgtcgtcgcc caaaccatca
     2641 atatttctct ttaaaagatg ataaatcagt cattcaggcg acaatgtggt caggtcactt
     2701 caaaaaattg ggttttgaat tagaggaagg gatgaaggtt aatgtagttg gccgtgttca
     2761 actttatgaa ccaagcggct cctactctat tattgttgaa aaagcagaac cggatggtat
     2821 aggagctctt gctatccaat ttgaacaact aaagaagaaa ctctctcaag caggttattt
     2881 tgatgatcgt cataagcaat taattcctca atttgttaga aaaataggag ttgtgactag
     2941 tccaagtgga gccgttattc gggatattat tacaacagta tctcgtcgat ttccaggagt
     3001 ggaaatatta cttttcccca caaaagttca aggagagggc gcagctcagg aaatagcaca
     3061 aactattgct ttagctaacg agaaaaagga tttggatcta ttaattgttg gccgtggggg
     3121 aggttctatt gaggacttat gggcatttaa tgaagaatgt gttgtagaag ctatttttga
     3181 atctcgtctt cccgttattt ctagtgtcgg acatgaaact gatacaactt tagcagattt
     3241 tgtagccgat cgcagagcag caactccaac tgcagcagct gagcttgcta caccggttac
     3301 taaaattgat atcttgtctt ggattactga acgtgaaaat agaatgtatc agtctagcct
     3361 tcgtcttatc aggacaaaag aggagaggtt gcaaaaatca aagcaatcag ttatttttag
     3421 gcaaccagag cgtctatatg atggtttcct tcaaaaatta gataacctaa atcagcagtt
     3481 aacatattct atgcgtgata aactacaaac agtaagacaa aagcaaggtt tgcttcatca
     3541 aaaactgcaa ggcatagatt taaaacagcg tattcatatt taccaagagc gtgttgtgca
     3601 aagtagacga ctattatcaa gtacaatgac tagtcagtat gacagcaagc tagcacgttt
     3661 tgaaaaggct caagatgcgc ttatatcatt ggatagttct agaattgtag cgcgtggtta
     3721 cgctattatc gaaaaaaacc atactttagt atctactact aatggaataa atgaaggaga
     3781 tcatttgcaa gttaagatgc aagatggtct tttagaagtt gaggtgaagg atgtcagaca
     3841 agaaaacatt tgaagaaaat ttacaggaat tagagacaat tgtctcacgt ttagaaacag
     3901 gtgatgttgc cttagaagat gccattgctg aatttcaaaa aggaatgctt atttcaaaag
     3961 agttacaaag aactttaaaa gaagctgaag agacgcttgt aaaagtgatg caagctgacg
     4021 gaacggaagt agaaatggat acttgatggt tactattgaa aaaattgatg aagctattca
     4081 tcgttattac aaacagacac atagtgtcgt ttctcctgat ttgattaagg ctatcctcta
     4141 ctcagttgat ggaggtggga aacgtattcg tccacgcatc cttttagaaa ttttagaggg
     4201 atttggagtt gaactaatag atggtcatta tgatgtggct gctgctttgg agatgattca
     4261 caccggctct ttaatccatg atgatttacc ggcgatggat aatgatgatt ttcgtcgtgg
     4321 ccgcttaaca aaccataaaa agtttgatga agctacagcg gttttagctg gagattccct
     4381 ttttttagac ccatttgatt tagttgtaaa agctggtttt aaagctgatg ttactgttag
     4441 gttaatagag ttattgtcta tgtctgcggg tagttttggc atggttggtg gacaaatgtt
     4501 agatatgaag ggagaaaaca aagttttatc tattgacgat ttaagcttga ttcatattaa
     4561 taaaacggga cgcctgttag cttatccctt tgttgcagca ggtattttag ctgagaagtc
     4621 ggaagaagta aaaggaaaac tgcatcaagc tggcctttta atcggtcatg cttttcaagt
     4681 acgtgatgat attttagatg tgactgctag ttttgaagaa ttggggaaga caccaaataa
     4741 agacattgta gcagaaaaga caacttatcc aaatttattg ggtttggata agtcacagga
     4801 aatacttgat gatactttga aaaaagctca ggcaattttt caaaatctag agaaaaaagc
     4861 taactttaat gctagaaaaa taatagatat aatagaggga ttacggttga atggctaaag
     4921 agagggtaga tgttcttgcc tataaacagg gactttttga tacacgagag caagcgaaac
     4981 gtggtgttat ggcaggaatg gtgattaacg ttatcaatgg agaacgttat gataaaccag
     5041 gtgaaaaggt tgcagacgat actgaattaa aactaaaagg tgaaaaacta aaatatgtta
     5101 gtagaggtgg attgaaatta gaaaaagctt tacaagtttt tgaaatttca gttgcagata
     5161 agctaactat agatattggc gcctctacgg gtggttttac tgatgttatg ctacaatcag
     5221 gagcgcgttt agtttacgca gtagatgtag gaacaaatca attagtttgg aagttacgtc
     5281 aggatcatcg tgttcgttct atggaacaat ataattttag gtatgcccaa aaagaagatt
     5341 tcaaggaggg actgcctgaa tttgcatcga tagatgtctc atttatctct cttaatttga
     5401 ttttaccagc tctaaaagaa attttagtgg atggtggaca agtagtggca ttaattaaac
     5461 cacaatttga agcaggtcgt gagcaaattg gtaaaaatgg tattgtcaaa gacaagttgg
     5521 ttcatgaaaa ggttttgaca acagtgacca atttcacgaa agattatgga tatacggtta
     5581 aacatcttga tttttcgccc attcaaggtg gacatggaaa tattgagttt ttaatgcatt
     5641 tgcaaaagtg tcaagatcca caaaatcttg tgcttgacca aatacaagat gttatagaaa
     5701 aagcacataa ggaatttaag aaaaatgaag aagagtgaac gtttaaattt aattaagcaa
     5761 attgttctta accatgctgt tgaaacacaa catgagttgt tacgcagatt ggaagcttat
     5821 ggggtaactc taactcaagc aactatttca cgtgatatga atgaaattgg cattataaaa
     5881 gtgccatcag caaaaggtcg ctatatttac ggtttgtcaa atgaaaacga ccctatcttt
     5941 acaactgctg tggcaaagcc tattaaaaca agtattttat caatatcaga taagctacta
     6001 ggtttagagc aatttatcaa tattaatgtc ataccaggta acagtcaatt aattaaaacc
     6061 ttcataatgt cacattgtca agaacatatt tttagtttga cagctgacga taatagtctc
     6121 cttttgattg caaaatcaga agcagatgct gatcacattc gtcaatcaat gattgcaatg
     6181 ttggagaaaa aagattagta gaggacctta tgcttttaga aatttctatt aagaatttcg
     6241 ctattatcga agaaatttca cttaattttg aaacaggaat gactgtttta actggtgaga
     6301 ctggtgcagg gaaatctatc attattgatg ctatgaatat gatgttagga tcccgtgcta
     6361 gcgttgaagt gattcgccat ggtgctaaca aagcagaaat tgaaggattt ttctctgttg
     6421 aaaaaaatca atcattagtc caattattgg aagaaaatgg cattgaatta gcagatgaat
     6481 taattattcg ccgagaaatc tttcaaaatg ggcgcagcgt tagtcgaatt aatgggcaaa
     6541 tggtgaattt gtcaacacta aaagctgtgg ggcattattt ggtagacatt catggtcagc
     6601 atgaccaaga ggaactaatg aaacctaaca tgcacattct gatgttagat gaatttggta
     6661 atacagaatt taatgtcata aaagaacgtt atcaaagtct ttttgatgct tatcgtcagc
     6721 ttcgtaaacg cgtattggat aagcaaaaaa atgaacaaga gaataaatca cgtattgaaa
     6781 tgctagaatt tcaaatagca gaaattgagt ctgtagccct taaatcagat gaagaccaaa
     6841 cgctactcaa gcaacgtgat aaattaatga atcataagaa tattgcagat actttgacaa
     6901 atgcatatct tatgttagat aacgaagagt tttcaagttt atcgaatgtt cgttctgcaa
     6961 tgaatgacct tatggcttta gaagaatttg atcgagaata taaagatctt tccaccaatc
     7021 tttcagaagc ttactacgtt attgaagaag ttactaaacg tttaggtgac gttatcgatg
     7081 atttagattt tgacgctggt ttactacaag aaattgaaaa tagactagat gttattaaca
     7141 caataacgcg aaaatatggc ggtgatgtga atgatgtttt agactatttt gataacatta
     7201 ctaaagaata tagtctattg acaggtagtg aagagtcctc agatgcttta gaaaaagaat
     7261 taaagatttt agaacacgat ttgattgaat cagcaaatca attaagttta gagcgccata
     7321 agctagctaa acaattggaa aatgaaatca aacaagaatt aacagagctt tatatggaga
     7381 aagctgattt ccaagttcaa tttacaaaag gaaaatttaa taaagaaggg aacgaaatcg
     7441 ttgaatttta tatttcaacg aatcccggag agggctttaa gccattggta aaagtggctt
     7501 caggaggaga gttatctcga ttgatgttag ctattaaatc tgctttttca cgtaaggaag
     7561 ataaaacttc cattgtattt gatgaggtgg atacaggagt ttctggtcgc gtggcccagg
     7621 ctatcgctca aaaaattcac aagattggta gtcatggaca agttttagca atttctcatt
     7681 tagctcaggt tattgcaata gcggattacc aatattttat tgaaaaaatt tccagtgact
     7741 cttcaacagt ttcaacggta aggcttttaa gctatgaaga acgtgtagaa gaaatcgcta
     7801 aaatgttggc aggaaacaat gtaacagaca ctgcacgtac tcaagctaaa gaattacttg
     7861 gtagttaact ttggtaacgt ctttaaaaag ctaacattgt taggagaaat taatgttgat
     7921 acacttaaat tttgttataa taagggtatt gaaatattaa agtatagaat aggataggat
     7981 atgagtaaaa tcaaaattgt aacggattct tccattacca ttgaaccaga acttattaaa
     8041 gagcttgaca ttacagttgt tcccttgtca gttatgatag atggtacctt gtattcagac
     8101 aatgacttaa aagcacaagg ggaattcttg aacctaatgc gtggaagtaa ggaattgcca
     8161 aaaacaagtc aaccacctgt aggtgttttt gcagaaattt atgaaaaatt aatgaatgaa
     8221 ggtgtggaac atatcattgc aattcattta acgcatacac tatcaggaac tattgaagca
     8281 tcacgccagg gagctaatat tgctggtgca gatgttacag ttattgattc tacttttaca
     8341 gaccagtgtc aaaaattcca ggttgtagaa gctgcgaaat tagctaaaga gggagctgat
     8401 ttagatacca tcttggctcg tgtggaagaa gtacgccaga agtcagaatt atttattggc
     8461 gtatcgaccc ttgaaaattt agtaaaaggt ggtcgtattg gacgtgtaac gggtcttcta
     8521 agttcattgc ttaatataaa agtaattatg gagctaacaa accatgaatt agtaccgatt
     8581 gttaaaggtc gcggtctaaa gaccttcagc aaatggttag ataattttgt tgaaagtgcc
     8641 caaacacgaa aaattgctga aattggcatc tcctactgtg gtaaagctga tatggctaat
     8701 aactttagag aaaaactagc tgttctaggt gctcctatat ccgttttaga aactggctca
     8761 atcatccaaa ctcatactgg tgaggatgca tttgctgtta tggttcgtta tgaataagaa
     8821 aaaaatactt accggacttt ctttttttct ggtaagtctt cttttatctt ttgggatttt
     8881 ttccttgatc attccaaaat caaatcctaa attaacaaaa aaagacttcc taacaaagaa
     8941 agttatccca cttaactatg ttgctcttgg agattctctg accgaaggtg tgggcgatac
     9001 aacctctcaa ggtggttttg ttccactgct atcagaatca ctccataatc gatactctta
     9061 ccaagtgact tctgttaatt atggtgtgtc tgggaatact agtcaacaaa ttttaaaacg
     9121 tatgacgaca gatcctcaaa tcgaaaaaga tttagagaaa gctgatttat tgacgctaac
     9181 tgttggtggt aatgatgtct tggctgttat tcgtaaagag ctcagtcatt tatcactaaa
     9241 ttcctttgag aaaccagcag aagcatataa ggaacgtttg aaagaaatcc ttgcaaaagc
     9301 aagacaagat aatcctaaat tgcctattta tgttttaggc atttataatc ctttttacct
     9361 aaactttcca caattaacta aaatgcaaac cgttattgat aattggaata aagctacaaa
     9421 agaagtagtt gatgcttcag aaaatgttta ttttgtccca attaatgacc gcctttataa
     9481 gggaataaat ggtaaagagg gtattacaga gtcatcaaat agtcaggcaa gtatcactaa
     9541 tgatgctctc tttactggag accattttca tcccaataat attggctatc aaatcatgtc
     9601 taacgccgtt atggagaaaa taaatgaaac aagaaaaaac tggccgtaat ttaaatttct
     9661 ggaaatgggc atttctctta ttgttggcaa ttaacctttc cttcacagca gtgattgcaa
     9721 gtcgcttaat tcaagtacgt gagcctaata caggaaaaat ttcgactggg gtacaagata
     9781 aagtaaaagt aggtactttt acgaccaata agtcgcaact gaataagaca attgcacttt
     9841 atttaaaaca atatcaaact aagaagatga attataagat ttatgctgct tcatcttcta
     9901 tactatttga aggctcttat caattattag gttatgaagt gcctttatac atctattttg
     9961 aaccttatcg tttaacaaat ggcgctgttc aacttaaagt gacctcattt tcagtaggaa
    10021 ctcttccgct tccagaaaaa gatgtgttac aatatataaa atcatcttat aaattgccaa
    10081 actttgtaga tataaaacct aaaaaatcgg ttattaatat taatttgcaa gatttaaaaa
    10141 ataaagaagg tatttatttg aaagcgactg caattgattt agtaaacgat aattttagtt
    10201 tcgatatttt taagaaaaag ccctaaaatg acgttaaata cttgacctta agcctttttt
    10261 tagatatcat ataatctgtt aaaacttatg ttgtataagt ttagaattta tttggaggat
    10321 ttgttaacat ggctaacaaa caagatttaa ttgcaaaagt agcagaagct acagaattaa
    10381 ctaaaaaaga ttcagcagca gcagtagatg cagtttttgc agctgtagca gattaccttg
    10441 ctgaaggtga aaaagtacaa ttaatcggtt ttggtaactt tgaagttcgt gaacgtgcag
    10501 ctcgtaaagg tcgtaaccca caaactggtg cagaaatcga aattgcagca tcaaaagttc
    10561 cagcatttaa agctggtaaa gctcttaaag acgctgttaa ataattcgtc tagaaaaacc
    10621 ttgtcatatc aatgtttatt gatagcgaca aggttctttt tttatatttg gggcatattt
    10681 ggggcataaa gttagtagtt ttccataata tcagctatgt tggatttcat tttctttgta
    10741 atgtgggtat aaatttgtac agtagttttt gcatcagcat gtccaacacg atccatgatt
    10801 gcttttagtg gtacgttatt ttcagcaaga cgactgacta aagtatgacg gaagatgtgg
    10861 ctagtgatat ttttattgat tggttgttca agccgctcgt ttgctttttt caatgctaaa
    10921 ttaaaggaat ttatttggat aggaacaccg ttcctagtcg tgaatatata ccccatgtct
    10981 ctgtatcgtt tatttgtatt tttttcgagt tcgttaataa actccatctc ttgtaaaatt
    11041 tccatttcac gtttgctcat aatggtttca cgataggacg caagagtttt tggagaggtc
    11101 ttttctccat ttatgtaacc attagtgcga tcataagttc cgtgcaactg tagagttttg
    11161 ttttcaaaat caatattatc cggctctatg ctaatagcct caccaatacg gcatccgtta
    11221 agactcataa attctgatag taatcccagg cggtgagtgc taggtcttct aaataattct
    11281 tttaacaacc gcttgatttc ttcttcttca agatattttt cttcaatctt tttccaatca
    11341 ttaagtgttt tcttgatttt tggcagttta gcgcgtcttg caggattatc cttgatgata
    11401 tctagactaa cagcataatc aaagataaga ttaagcatag acttgtttcg ctcttttttg
    11461 tttctagagc aatcgagatt atccagataa ttttggacat atctagggtc gatattaact
    11521 acaggtatat tgatcccaaa ttctgctcta atctctcgaa tattgccttt taaagtgaca
    11581 atagacgatc ttttaatttc ctgttggtaa aatatccacc actcatcaat tagatttgta
    11641 aatagcatgt cagaggtttg caactttctt acgatatcag ctatcttagc atctaatatt
    11701 ctttgagctt cttttctaat tcgaggagta tctttttcca tcagtactga tgtacgtttc
    11761 catttattag tatatggatc tttatatctt tctacaaaat ttacttttcc acttttgtgt
    11821 ttttctgacc acattgtttt ttacctcatt ttttgttaaa atgggtacaa taaaaagggc
    11881 tttttaatgc ctgatttatt gtacagtttt gcctcacgct ctcctcgcca aatttgagcg
    11941 tggggctttt tttatttaaa gaaattatta acatttacgt actgagcctg cgtaagctta
    12001 gtttttaagg ttttaacttc tccggtttgt atatttctta aggttatcat tgcttttcca
    12061 ggtatttctt cagaagtagt tgtagcagta gtgttaatgg ttcctttttt ctttctagcc
    12121 ccaccagcca ttccaccgat aatggttcca acgcctagcg ccaaaactgt gccgattgct
    12181 gctcctccta gaacactacc agaacgccct ttttgagtag ttttcccttt tgttactgtt
    12241 ttttcaatga ttcgtgagcc atcaaattga aggttaacaa actcataaag ctcaggtttt
    12301 tcagaataaa aaccaatata gtagtcaccc tccactgttt tacgaatagc agtaggtgta
    12361 ttaaaaccta aattaattgt gggcggtaag actattttgc tttcttgtga tgcccttttt
    12421 ctcatttcgt ttgctttaat aatgccgtct ttagcctttt ctgatgttga cacagcaaat
    12481 tcttttattt tatttatatc catataatct cctcactata ttacacgaca ttaagtaaat
    12541 tatagtattc atcaattacc atgcattcat ctgcagtagt tgttagttga tgatattgca
    12601 taaattgtag gtagttaaac tctcgtttat cttctaattg agaaagtgct tcagcgatta
    12661 aatgactaat catgtatctg tcagcttcta gctcgtgttt ctcacgtagt gtcttatagt
    12721 caccaatatt tctttcacga tgtccgagtt catgcaggat aacccgtttt tgagcatcta
    12781 acgatagtga tttattgaca aaaataactt taatatcgtc tatataaatc ccaggtcgtg
    12841 gccataattc gttatcaaag tatgcgagat taactttgtg acagtctaca atctcttcaa
    12901 tagtcataga ctatcttccc ttcaagtaaa tttcaatgat gttttgaatg gcattgatgt
    12961 catcttctgt caatggttta ccatcgaacg tttttgcatt ctcagccatt ttgcgcaagt
    13021 cttcagtaga atatccagtt acttcatcag acgctattct agggttatct gtgcgtccat
    13081 agatgtagtc agtagatacc cggaagtagt cagctatttt tgtgattgct tcactagatg
    13141 aagatttccg tgtccttaaa ttataaatat aattatcact caaaccaaca gcaatcgaaa
    13201 cagtttgcaa actcactcct tgcttgtctg ctaaattttt aatcctctca aaagcgatta
    13261 gattcaatgt ttgttcagtc atttaaagcc tttctaagcc atgacaaaaa aattttacac
    13321 ctatgagtaa aaaactattg acataattaa ctcaaacgag taaaatataa aatgtaaagc
    13381 gaataaataa gcgaaacaaa aagcgaaata aaaaaactaa aaaataagtt tggcgacttt
    13441 ggaaattagt attattgagc tattttgtta gtgtttttc
//
LOCUS       DATRMA010000047        13190 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.047, whole genome shotgun sequence.
ACCESSION   DATRMA010000047 DATRMA010000000
VERSION     DATRMA010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13190
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.047"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            complement(<1..319)
                     /locus_tag="VBC28_001362"
     CDS             complement(<1..319)
                     /locus_tag="VBC28_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001127191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEO6815344.1"
                     /translation="MPKVLLGFMGVGKTSVANCLENEVIDMDSLIEKHIGMSISRFFT
                     EEGEASFRALESQFLNELLKKKNEGLVIASGGGIVLLEENRRLLTLNRHNNILLTGSF
                     EVLY"
     gene            complement(312..1595)
                     /gene="aroA"
                     /locus_tag="VBC28_001363"
     CDS             complement(312..1595)
                     /gene="aroA"
                     /locus_tag="VBC28_001363"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEO6815345.1"
                     /translation="MKLLTNANTLKGTIRVPGDKSISHRAIIFGSISQGVTRIVDVLR
                     GEDVLSTIEAFKQMGVLIEDDGEIITIYGKGFAGLTQPNNLLDMGNSGTSMRLIAGVL
                     AGQEFEVTMVGDNSLSKRPMDRIALPLSKMGARISGVTNRDLPPLKLQGTKKLKPIFY
                     HLPVASAQVKSALIFAALQTKGESLIVEKEQTRNHTEDMIRQFGGHLDIKDKEIRLNG
                     GQSLVGQDIRVPGDISSAAFWIVAGLIIPNSHIILENVGINETRTGILDVVSKMGGKI
                     KLSSVDNQVKSATLTVDYSHLQATHISGAMIPRLIDELPIIALLATQAQGTTVIADAQ
                     ELKVKETDRIQVVVESLKQMGADITATADGMIIRGNTPLHAASLDCHGDHRIGMMIAI
                     AALLVKEGEVDLSGEEAINTSYPNFLEHLEGLVNA"
     gene            1824..2888
                     /locus_tag="VBC28_001364"
     CDS             1824..2888
                     /locus_tag="VBC28_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HEO6815346.1"
                     /translation="MKRKQFIKLGIATLLTVISLYTPINLATNHTTENIVTAQEYKTK
                     ENGTLPFKHKRQLVLGELDDKGRATFAHIQLKVKDEPKKKRVKRLKTTPVGWHNFKFY
                     YNDGTQKAWLVSRGRLICHQFSGLNNERKNLVPMTNWLNTGNYNSTNSSNPESMLFYE
                     KQLKTWLSTHKNYYLDYKVTPIYQNNELIPRKIELKYVGIDKTGKLLPIFIGNKSTQD
                     QFGISTVTLENTSPNATIDYLSGKAQNTVLSAKEQRKLIAKHEEEKRLAEKKVEEEKA
                     AAETQKKLEEEQARLAAEAQRKQKEEQARLAAETQKKQETLVQEQTSQGYKRDYRGRW
                     HRPNGQYASKAEIAAAGLQW"
     gene            complement(2996..4303)
                     /gene="eno"
                     /locus_tag="VBC28_001365"
     CDS             complement(2996..4303)
                     /gene="eno"
                     /locus_tag="VBC28_001365"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEO6815347.1"
                     /translation="MSIITDVYAREVLDSRGNPTLEVEVYTESGAFGRGMVPSGASTG
                     EHEAVELRDGDKSRYGGLGTQKAVDNVNNVIAEAIIGYDVRDQQAIDRAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYSYLGGFNTKVLPTPMMNIINGGSHSDAPI
                     AFQEFMIMPVGAPTFKEALRWGAEVFHALKKILKERGLETAVGDEGGFAPKFEGTEDG
                     VETILKAIEAAGYEAGENGIMIGFDCASSEFYDAERKVYDYSKFEGEGGAVRTAAEQI
                     DYLEELVNKYPIITIEDGMDENDWDGWKALTERLGGRVQLVGDDFFVTNTDYLARGIK
                     EEAANSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTIADIAVATNAG
                     QIKTGSLSRTDRIAKYNQLLRIEDQLGEVAQYKGIKSFYNLKK"
     gene            4501..4956
                     /locus_tag="VBC28_001366"
     CDS             4501..4956
                     /locus_tag="VBC28_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HEO6815348.1"
                     /translation="MTELDNKILQKASGENRLDPDQQRYYLGTYKERVVLHLSLKEAQ
                     EEKFQLAITSALNNLILEYPNLSLKLSPNLPTDVQMFYMKLANRLSLAATIVNEANAN
                     SPFGIIIHSDKAENVEWNDFETQFPDLFNSPKKEESPKKSLWQHFFSQK"
     gene            4969..5454
                     /locus_tag="VBC28_001367"
     CDS             4969..5454
                     /locus_tag="VBC28_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000409123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEO6815349.1"
                     /translation="MEIWDAYDQYGFITGLTLNRDQNIPQGLFHLVVDVILFHEDGDV
                     LMMKRHPKKKAFPAYFEATAGGSALKGENAKQAILRELKEETGIVPQCLTFLNREWFS
                     ERSYFVDHFIAKYNGAKDIITLQEGETVDYIWLKPEYIDLFLSKNKLIPSQIKLLKSL
                     I"
     gene            complement(5475..6116)
                     /gene="serB"
                     /locus_tag="VBC28_001368"
     CDS             complement(5475..6116)
                     /gene="serB"
                     /locus_tag="VBC28_001368"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HEO6815350.1"
                     /translation="MIKGLLVMDVDSTLIMEEAIDLLAIEAGVGKQVAALTDAAMRGE
                     LDFEEALKKRVALLKGLPVTILTDILSSIHFTPGAYELIKECHKRQMKVGLVSGGFHE
                     TIDILAKQLQVDYVKANRLGVKGGFLTGEVEGEIVTKEVKKIKLKEWASENHLDLSQT
                     IAMGDGANDLPMIKSAGVGIAFCAKPIVREEAAYQINEKNLLKVFDIIDANEN"
     gene            complement(6209..7933)
                     /gene="ezrA"
                     /locus_tag="VBC28_001369"
     CDS             complement(6209..7933)
                     /gene="ezrA"
                     /locus_tag="VBC28_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HEO6815351.1"
                     /translation="MSSGIILLLVAIVLLVIIAYVVGVVIRKRNDTLIANLETRKQEL
                     VDLPVQEEIEQVKLLHLIGQSQSTFREWNQKWTDLSTNSFKDIDFHLVEAENLNDSFN
                     FVRAKHEIDNVDSQLTIIEEDIVSIREALEVLKEQEEKNSARVTHALDLYETLQKSIS
                     EKEDNYGTTMPEIEKQLKNIEAEFSHFVTLNSTGDPIEASEVLNKAEEHTIALGQITE
                     QIPAIVAKLEDDFPDQLDDLETGYRRLLEENYHFPEKDIEQRFQEVREAIRSNSDGLV
                     SLDLDRARDENEHIQEKIDKLYDIFEREIAAYKVAHKDSKIIPQFLAHAKSNNEQLGH
                     EIKRLSAKYILNENESLSLRSFTNDLEEIETKVLPSVENFGQEASPYTHLQILFERTL
                     KTLTTVEENQMEVFEAVKTIESVETRARQNMDKYVNKLHMIKRFMEKRNLPGIPQDFL
                     STFFTTSSQIEALINELSRGRIDIEAVSRLNDVTTNAIANLEQATYLVVQDATLTEQL
                     LQYSNRYRSFEQNVQKSFEQALYLFEVEHNYKASFDEISYALETVEPGVTDRFVTSYE
                     KTQERIRF"
     gene            complement(8027..9979)
                     /gene="gyrB"
                     /locus_tag="VBC28_001370"
     CDS             complement(8027..9979)
                     /gene="gyrB"
                     /locus_tag="VBC28_001370"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002283665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEO6815352.1"
                     /translation="MTEETKNMEQRAQEYDASQIQVLEGLEAVRMRPGMYIGSTSKEG
                     LHHLVWEIVDNSIDEALAGFAGHIKVYIEPDNSITVVDDGRGIPVDIQEKTGRPAVET
                     VFTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKNGKVHYQEYQRGVVV
                     NDLEIIGDTDLSGTTVHFTPDPEIFTETTVFDFDKLAKRIQELAFLNRGLRISISDKR
                     EGQEVEKEYHYEGGIGSYVEFINENKEVIFENPIYTDGELDGISVEVAMQYTTGYQET
                     VMSFANNIHTHEGGTHEQGFRTALTRVINDYAKKNKILKENEDNLTGEDVREGLTAVI
                     SVKHPNPQFEGQTKTKLGNSEVVKITNRLFSEAFNRFLLENPQVAKKIVEKGILASKA
                     RIAAKRAREVTRKKSGLEISNLPGKLADCSSNNAEMNELFIVEGDSAGGSAKSGRNRE
                     FQAILPIRGKILNVEKATMDKILANEEIRSLFTAMGTGFGADFDVSKVRYQKLVIMTD
                     ADVDGAHIRTLLLTLIYRFMRPVLEAGYVYIAQPPIYGVKVGSEIKAYIQPGVNQEEE
                     LRQALDTYSSGRSKPTVQRYKGLGEMDDHQLWETTMDPENRLMARVSVDDAAEADKIF
                     DMLMGDRVEPRREFIEANAVYSNLDI"
     gene            complement(9980..10540)
                     /locus_tag="VBC28_001371"
     CDS             complement(9980..10540)
                     /locus_tag="VBC28_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001106189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEO6815353.1"
                     /translation="MNYHDYIWDLGGTLLDNYESSTRAFVETLKEFGYQADHDSVYQK
                     LKESTADAIAYFIPEEADFLKEYKANEAKVLETPILFQGAKELLAKIQRQGSRNFLVS
                     HRDNQVIVILEKTEIIDYFTEVVTADNGFSRKPSPESMLYLKEKYQIDNCLVIGDRDI
                     DKQAGESAGFDTLLVDGSKSLMEIIE"
     gene            complement(10659..11864)
                     /locus_tag="VBC28_001372"
     CDS             complement(10659..11864)
                     /locus_tag="VBC28_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HEO6815354.1"
                     /translation="MSQKSNYFKGQIDYAVVIPVFFLLMIGLASIYVATMNDYPSNIY
                     IAMFQQVSWIIMGCIIAFVVMLFSTEFLWKATPYLYALGLTLMVLPLIFYSPQLFAAT
                     GAKNWVTIGSVTLFQPSEFMKISYILMLSRITVSFHQKNRKTFQDDWKLLGLFGLVTL
                     PVMILLMLQKDLGTALVFLAILSGLILLSGISWWIILPILSTIVLFIASFLMIFISPN
                     GKEWFYNLGMDTYQINRLSAWIDPFSFADSIAYQQTQGMVSIGSGGVTGKGFNILELS
                     VPVRESDMIFTVIAENFGFIGSAIVLGLYLIIIYRMLRITIESNNQFYTFISTGFIMM
                     IVFHVFENIGAAVGILPLTGIPLPFISQGGSSLLSNLIGIGLVLSMSYQNTVRQAKAN
                     GSHLKYRSI"
     gene            complement(12289..12477)
                     /locus_tag="VBC28_001373"
     CDS             complement(12289..12477)
                     /locus_tag="VBC28_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Paratox"
                     /protein_id="HEO6815355.1"
                     /translation="MSIRTDIDEFKEAIDKGYISGNTVAIVRKNGKIFDYVLLHEEVR
                     EEEVVTVERVLDVLRKLS"
     gene            complement(12519..12719)
                     /locus_tag="VBC28_001374"
     CDS             complement(12519..12719)
                     /locus_tag="VBC28_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEO6815356.1"
                     /translation="MSQEKLKSKLDQAKGGAKEGFGKITGDKELEAKGFIEKTIAKGK
                     ELADDAKDAVEGAVDAVKEKLK"
     gene            12897..13190
                     /locus_tag="VBC28_001375"
                     /pseudo
     CDS             12897..13190
                     /locus_tag="VBC28_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612023.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
BASE COUNT         4258 a         2634 c         2040 g         4258 t
ORIGIN      
        1 gatataagac ttcaaaactt cctgttaaaa gaatattatt atgacgattt aaagttaaaa
       61 gacgacgatt ttcttcaagg agtacaatac ctcctccact agctatcaca agtccttcat
      121 tttttttctt taaaagttcg tttaaaaatt gagattctaa agcacgaaag gaggcttctc
      181 cttcttctgt aaaaaatcgg gaaattgaca taccaatatg tttttcaatc agactatcca
      241 tatcaattac ttcattttct aagcagttgg caacactagt cttgccaact cccatgaatc
      301 ccaataatac cttaggcatt aactaatccc tccaaatgtt ctaaaaagtt aggatagctt
      361 gtattaatag cttcttcacc agataaatct acttcgcctt ctttgaccaa gagagcagca
      421 atagctatca tcattcctat tctatgatct ccatgacaat caagtgaagc agcgtgaaga
      481 ggggtattac cacgtataat cataccatct gctgtagcag taatatcagc acccatctgt
      541 tttagcgatt cgacaacgac ctgaatccga tctgtctctt tcacctttaa ttcctgagca
      601 tctgctatga ctgtggtgcc ttgagcttgt gttgctaaaa gagcaataat aggtaattca
      661 tcaatcagac gtggaatcat agccccagaa atatgagtag cttgtagatg agagtaatca
      721 actgtaaggg ttgctgattt tacttgatta tcaaccgaag ataacttaat ttttccaccc
      781 attttactga cgacatcaag gattccagtt cgtgtctcgt ttatacctac gttttctaaa
      841 ataatatgac tgttaggaat aataagaccc gctactatcc aaaaggcagc actcgaaata
      901 tcaccaggaa ctcttatatc ttgtcctact aagctctgac cgccattgag tcgaatttct
      961 ttatctttaa tatccaaatg tccaccaaat tgccgaatca tgtcctcagt atgattcctt
     1021 gtttgctctt tttctacaat gagagattct cccttagttt gtaaagccgc aaaaattaaa
     1081 gctgatttta cttgtgcaga agctacgggt aaatggtaaa aaataggttt taattttttt
     1141 gtaccttgaa gttttaatgg tggtaaatca cggttagtta ctcccgaaat tctagctccc
     1201 attttgctta aaggcaatgc tatacgatcc ataggtctct ttgacaaact attgtctcca
     1261 accatcgtca cttcaaactc ttgaccagct aacacacctg ctatcagacg cattgaagta
     1321 ccagagtttc ccatgtctaa gagattattc ggttgtgtta aacctgcaaa tcctttgcca
     1381 tagatagtga ttatctcgcc atcgtcttca attagaaccc ccatttgttt aaaagcctct
     1441 atagttgata aaacatcctc gcctcttaag acatcaacaa ttcgggtaac tccctgagat
     1501 atactcccaa aaataatagc cctgtgactt attgatttat cgccaggaac acggatggtt
     1561 cccttgagag tatttgcatt agtcagtaat ttcatgataa ctcccaaaat ctattttgct
     1621 attattgtat cataatatat gctatatttt gaatttttta tttatactat agctcctcta
     1681 tatagctgta acatctttta taaattaact tcacatgtca tacatcatta tgttataata
     1741 ttgttacaaa aatcacattt taattttcag aatgcattta aaaataaaca gctactattt
     1801 tatgttattt tagaggttaa cagatgaaaa gaaaacagtt tataaaatta ggaattgcaa
     1861 ccttactaac ggttatttcg ctttacacac caataaacct agctacaaat cataccacag
     1921 aaaatattgt tactgctcaa gagtataaaa caaaagagaa tggtacttta ccttttaaac
     1981 acaaacgaca attggtttta ggagagttgg atgacaaagg tcgtgccaca ttcgcacata
     2041 ttcagctaaa agttaaagat gaacccaaga aaaagagagt aaaacgttta aaaacaaccc
     2101 ctgttggatg gcacaatttt aaattctatt ataatgatgg tactcaaaaa gcttggctag
     2161 tgagccgagg ccgtcttatt tgccaccaat ttagtggact aaacaatgaa agaaagaatt
     2221 tagttccaat gacgaattgg ctaaatactg gaaactacaa tagcacgaat tcatctaacc
     2281 ctgagagcat gctattctat gaaaaacaac taaagacttg gctttcgact cataaaaact
     2341 actacttaga ttataaagta acaccaattt atcaaaataa tgaactaatt ccaagaaaaa
     2401 ttgaattaaa atatgttggt attgataaga ctgggaaatt attacctatt tttatcggaa
     2461 acaaatccac tcaagatcaa ttcggcatca gcactgttac cttagagaat acctcaccaa
     2521 atgctaccat tgactacctc agcggaaagg ctcaaaatac tgtcttatca gctaaagaac
     2581 aaagaaaact tatcgctaag cacgaagaag aaaaacgact tgcagaaaag aaagttgaag
     2641 aggaaaaagc tgctgctgaa actcagaaaa aactggaaga agaacaagca aggcttgctg
     2701 ctgaagctca aaggaagcaa aaagaggagc aagcaaggct cgcagctgaa actcagaaaa
     2761 aacaagaaac tctagtacaa gaacaaacta gtcaaggcta caaacgtgac tatagaggaa
     2821 ggtggcatcg tccaaatgga caatacgctt caaaagcaga aatcgctgct gctggattgc
     2881 aatggtagaa ctgtttatta tcaaatcaat tctattatta taatgtgtac ataaaaacca
     2941 tcttgcatat gcaagatggt ttttattata ttttcaactg tttaataatc tgagactatt
     3001 tttttaagtt gtagaatgat ttgatacctt tgtattgagc aacttcacca agttggtctt
     3061 cgatacgaag taattggttg tatttagcaa tacggtcagt acgtgataat gaacctgttt
     3121 taatttgacc agcgttagtt gcaactgcga tatcagcgat tgttgaatct tcagtttcac
     3181 ctgaacggtg tgatacaact gcagtgtaac cagcttcttt agccatttca atagcttcga
     3241 aagtttctgt caaagtaccg atttggttaa ctttgataag gattgagtta gcagcttctt
     3301 ctttgatacc acgtgctaag taatcagtat ttgtaacgaa gaaatcgtca ccaactaatt
     3361 gtacacgtcc accaaggcgt tctgtaagag ctttccaacc atcccagtcg ttttcatcca
     3421 taccatcttc aatagtgatg attgggtatt tgttaaccaa ctcttcaagg taatcgattt
     3481 gttctgcagc agtacgaact gctccacctt caccttcgaa tttactgtag tcgtatactt
     3541 tacgttctgc atcgtagaac tctgatgaag cacagtcaaa accaatcatg attccgtttt
     3601 cgccagcttc gtaaccagct gcttcaatag ctttaagaat agtttctacg ccatcttcag
     3661 taccttcaaa tttaggtgca aatccacctt catcaccaac agctgtttca agaccacgtt
     3721 ctttaaggat tttcttaaga gcatggaata cttcagcacc ccaacgaagt gcttctttga
     3781 aagttggtgc accaactggc ataatcatga attcttggaa tgcgattgga gcgtctgagt
     3841 gagaaccacc gttgatgatg ttcatcattg gagttggaag aactttagtg ttgaatccac
     3901 caaggtagct gtaaagtggt acttcaaggt aatcagctgc agcacgtgct acggcaatag
     3961 aaacaccaag aatagcattt gcacctaatt tacctttatt aggagttccg tcaagagcga
     4021 tcatagcacg gtcgatagct tgttggtcgc gtacatcata accaatgatt gcttctgcaa
     4081 taacgttatt tacattgtca acagcttttt gtgttccaag accaccgtaa cgagacttgt
     4141 cgccgtcgcg aagttcaact gcttcgtgtt caccagtaga agctcctgaa ggaaccatac
     4201 cacgtccgaa agcacctgat tctgtataaa cttctacttc aagtgttggg ttaccgcgtg
     4261 agtcaaggac ttcgcgtgcg taaacatcag taataattga cattacaata ctctccttat
     4321 gagttttaat tttttacata ttcataatat cataatcttt agttaataac aaggaaaaac
     4381 ccagtgaaac cgctttatta cgtggttttt gataaaaaaa tcactaattc ttttcaagtt
     4441 tacctgtgct aatgcaaccc atttaggtta taataaaatt agcaaattca aggagaaatt
     4501 atgactgaac ttgacaataa aatcctacaa aaagcaagcg gtgaaaatcg tcttgatcct
     4561 gatcaacaac gttactacct tggtacttat aaagaaagag ttgtgctcca cttaagttta
     4621 aaagaggcac aagaagaaaa gtttcaactt gctataacat ctgctcttaa taatctgata
     4681 cttgaatatc ctaatcttag tttaaaatta tcaccaaatc tccctactga tgttcaaatg
     4741 ttctacatga agttagctaa ccgtctatca ctagctgcta cgattgttaa tgaagcaaat
     4801 gccaattctc catttggtat tattatacat agtgataagg ctgaaaacgt tgaatggaac
     4861 gattttgaga cacaattccc tgatttattc aattcaccaa aaaaagagga gtctcctaaa
     4921 aaaagtctat ggcaacattt ctttagtcaa aaataacaag gaggtctaat ggaaatttgg
     4981 gatgcttatg accaatatgg gttcattaca ggtcttacac ttaataggga tcaaaatatc
     5041 ccacaaggct tattccactt agttgttgat gttatccttt ttcatgagga tggtgacgta
     5101 ttgatgatga aacggcaccc taaaaagaaa gcttttcctg cttatttcga agccaccgct
     5161 ggcggatctg cccttaaagg agaaaatgct aagcaagcta ttctaaggga actcaaagaa
     5221 gaaactggta tcgttcctca atgtctaacc tttcttaata gagagtggtt ctctgagcgt
     5281 tcttactttg tagaccattt tatcgcaaaa tacaatggtg caaaagatat aattacattg
     5341 caagaaggag agaccgttga ctatatctgg ttaaaaccag aatatattga tttattttta
     5401 tctaaaaaca aattaatacc ctctcaaata aaactcttaa aatccttaat ctaaaaacta
     5461 ggcttaataa aggcctagtt ttcatttgca tcaataatat caaatacctt aagcaaattt
     5521 ttttcattaa tctgatacgc agcttcttct ctgacaattg gttttgcaca aaaagcaatt
     5581 cctaccccag cggatttgat cattggtaaa tcattggcac catctcccat tgcgatggtt
     5641 tgtgaaaggt ctaagtgatt ttcagaagcc cactctttta actttatttt tttcacttct
     5701 ttagtgacaa tctctccctc aacttcgcca gtgagaaaac cacctttgac accaagacga
     5761 ttcgctttga cataatcaac ttgtaattgc ttagctaaga tatcaatcgt ctcgtgaaaa
     5821 cctcctgata caagaccaac tttcatctga cgcttgtgac actctttaat caattcataa
     5881 gcccctggag taaaatgtat tgaggataat atatctgtca aaatagtaac aggcaaacct
     5941 ttaagtagcg ctactctttt cttcaaagct tcttcaaaat ctaactcgcc acgcattgca
     6001 gcgtcagtca gtgcagcaac ctgtttcccc acccctgctt caatagctaa taaatcaata
     6061 gcttcttcca ttattaaggt tgaatcaacg tccatgacta acaaaccttt aatcatttaa
     6121 aacctccaaa acttttttac tattgtacct taaaattagc accttcatag atagcaatta
     6181 aaaaaagaag agtactaact cttctttttt agaaacgaat acgttcttgt gtcttttcat
     6241 aagacgtcac aaaacggtca gttacacctg gttccacagt ctcaagtgcg tatgaaattt
     6301 catcgaaaga tgctttatag ttatgttcaa cttcaaataa gtacaaagct tgttcaaagc
     6361 tcttttgcac attttgttca aaactacgat aacggtttga atactgtagt aactgctctg
     6421 tgagagtcgc atcttgaaca actagatatg tagcttgctc taaattagcg atagcatttg
     6481 tagtcacatc atttaaacgt gaaacagcct caatatcaat acgaccgcga ctgagttcat
     6541 taataagtgc ctcaatttga gaacttgttg taaagaaagt gcttaagaag tcttgcggga
     6601 ttcctggaag attgcgtttt tccatgaaac gtttaatcat atggagcttg ttaacatact
     6661 tatccatatt ttggcgtgct cgtgtctcaa cgctttcaat agttttaacg gcttcaaaaa
     6721 cttccatttg attttcttca acagtagtca atgttttcaa cgtacgctca aataaaattt
     6781 gtagatgagt ataaggtgaa gcttcttgac caaaattttc tacagaaggt aaaacctttg
     6841 tttcaatttc ttccaaatca tttgtgaagg aacgtaaact taagctttca ttttcattta
     6901 agatatattt agcagataat cgtttgattt catgccccag ttgttcattg ttagattttg
     6961 catgagcaag gaattgagga ataattttac tatccttatg agctacttta taagcagcaa
     7021 tctcacgttc aaaaatatca taaagtttat caattttttc ttgaatatgt tcattttcat
     7081 cgcgagcacg atctagatcc aaactaacta aaccatctga attcgaacga atagcctcac
     7141 gaacttcttg aaaacgttgt tcaatatcct tttcagggaa atggtagttt tcctctaaaa
     7201 gacgacgata tcctgtctcc aaatcatcta actgatctgg aaaatcgtct tctaatttag
     7261 caacaatagc tggaatttgt tctgtaattt gccctaaagc aatcgtatgc tcctctgctt
     7321 tattcaaaac ttcagaagct tcaatcgggt caccagttga gtttaaggtc acaaaatgtg
     7381 aaaattcagc ctcgatattt ttcaactgct tttcaatttc tggcatggtt gtcccgtaat
     7441 tatcttcttt ttcagaaatt gatttttgaa gtgtttcgta taggtccaaa gcatgtgtta
     7501 cacgggcact atttttctct tcttgctctt ttaaaacttc taaagcttca cgaatcgaaa
     7561 caatatcttc ttctataata gttagctgac tatcaacatt atcaatctcg tgcttagcac
     7621 gcacaaaatt aaatgaatca ttaagatttt cagcttcaac taaatgaaaa tcaatatctt
     7681 taaatgaatt agttgataaa tcagtccatt tttggttcca ttctctaaaa gttgattgac
     7741 tttggccaat caaatgaagc aatttgactt gctcaatctc ctcttgcaca ggaaggtcaa
     7801 ctaactcttg ttttctagtt tctaagtttg caattaatgt atcattacgt tttcttatta
     7861 cgacgccgac tacgtaggca ataatcacaa gcaggactat agccacaagc agcaatatga
     7921 ttccgctcga cattcaactc tccttataat ggtctaattt tctaaaaatc tactataacg
     7981 ttaagattat agcatacttg aactgttttt caaagctatc tttggactag atatctaagt
     8041 tactataaac agcattagct tcaatgaatt cacgtcgtgg ttctactcga tctcccatta
     8101 gcatatcgaa aatcttatct gcttctgctg catcatcaac tgagacacgt gccatcaagc
     8161 ggttttcagg atccattgtt gtttcccaaa gttgatggtc atccatctca cctagacctt
     8221 tgtaacgttg tacagtaggt tttgaacgtc ctgaactata ggtatctaga gcttgacgta
     8281 attcctcttc ttgattgacc cctggctgaa tataagcttt aatctcactt cctactttga
     8341 caccatagat tggtggttga gcgatataaa catatcctgc ttccaaaaca ggtcgcataa
     8401 aacgataaat cagagttaaa agtaaagttc tgatgtgagc tccatcaaca tcggcatcag
     8461 tcataatgac taatttttga tatctgactt ttgatacatc aaaatctgca ccaaatccag
     8521 ttcccatagc tgtgaagaga cttcggattt cttcgtttgc taaaatttta tccattgtag
     8581 ctttttcaac gtttagaatc ttacccctaa tcggtaaaat tgcttgaaac tcacgattac
     8641 gtcctgattt tgcagaccct cccgcagaat ctccctccac tataaaaagt tcattcatct
     8701 cagcattatt agatgagcaa tcagccagtt tcccaggtaa gtttgaaatc tctaagcctg
     8761 actttttacg tgttacttca cgcgcccgtt ttgctgcaat acgggcttta gatgccaaaa
     8821 ttcctttttc aacaatcttc ttagctactt gtggattttc taataggaat cgattgaaag
     8881 cttcgctaaa gagacgattt gtaattttaa caacttcaga atttccaagt tttgttttag
     8941 tttgtccttc aaattgtgga tttggatgtt taacagatat aactgctgtt aaaccttcac
     9001 gaacatcttc tccagttaaa ttatcttcat tttctttgag aatcttattc tttttagcgt
     9061 aatcattaat cacacgtgtc aaggcagtac gaaatccttg ttcgtgcgtt cctccttcat
     9121 gagtgtggat gttatttgca aaactcataa cagtctcttg atatccagta gtatactgca
     9181 ttgctacttc aactgaaata ccatctaatt caccgtcagt ataaatagga ttttcaaaaa
     9241 tcacttcttt gttctcattg ataaactcaa cgtatgaacc tataccacct tcatagtgat
     9301 attctttttc aacttcttga ccttctcgct tgtctgaaat ggagattctt agtccacgat
     9361 ttaaaaaagc taattcctga atacgtttgg ctaatttatc aaaatcaaat acagtggttt
     9421 cagtgaaaat ttctgggtca ggcgtaaagt gaactgttgt tcctgataag tcagtatcac
     9481 cgataatttc aagatcatta acaacaacac cgcgttggta ttcttgataa tgaaccttgc
     9541 catttttata aaccttgaca tctaattgtg ttgacaatgc attaacaaca gatgacccta
     9601 caccatgtaa gccaccagaa accttgtatc cgccaccacc aaatttacca ccggcatgga
     9661 gaactgtaaa aactgtctct acagctggtc ttccagtttt ttcttggata tctaccggaa
     9721 taccgcgacc atcatcaaca actgtaatag agttatctgg ttcaatgtaa actttaatat
     9781 gtcctgcaaa tccagctaag gcttcatcaa ttgagttatc aacaatttcc catacaaggt
     9841 gatgaagccc ttctttcgaa gtcgaaccga tatacatgcc tggacgcatg cgaacagctt
     9901 ctaaaccttc taatacttga atctgacttg cgtcatattc ttgagcacgt tgttccatat
     9961 tctttgtttc ttctgtcatt tactctatta tctccatcaa tgattttgac ccatcgacta
    10021 ataaggtatc aaaaccagcc gattctcctg cttgcttatc tatatcacga tcgcctatta
    10081 ctaaacaatt atcaatttgg tatttttctt tcaaataaag catactctcg ggactaggtt
    10141 tccttgaaaa gccgttatca gcagtcacta cttctgtaaa atagtcaata atttctgttt
    10201 tttccagaat aactataact tgattatcac gatgagacac caaaaaatta cgactacctt
    10261 gtctttgaat tttcgctaaa agttcttttg ccccttggaa tagaattggt gtttccaaaa
    10321 ccttagcttc attagcctta tactctttta aaaaatcagc ttcttcaggg ataaaatatg
    10381 caattgcatc tgctgttgac tcttttaatt tttgatacac actatcgtgg tctgcttgat
    10441 atccaaattc tttcaaagtc tctacaaaag cacgtgtgga actttcgtaa ttatcaagta
    10501 gtgtaccacc caaatcccaa atataatcat gataattcat accctttatt atatcatatt
    10561 attacgattt tttatagcta ccaccttact taattaactc actcctctaa aatatttcga
    10621 ttattaaaag gctggattat ctttccaacc tttttttgtt atatactacg atacttcaaa
    10681 tgtgatccat tagccttggc ctgacgaact gtattttgat aagacatgga taagaccaat
    10741 ccaattccaa taagattact tagtaatgaa cttccacctt gcgaaataaa cggaagtggg
    10801 attcctgtta gaggtaatat accaactgct gcaccaatat tttcaaaaac atggaaaaca
    10861 atcatcatga taaaaccagt tgatataaaa gtataaaatt gattattaga ttcaatggtg
    10921 atccttaaca tacggtaaat gataatgaga tataacccta aaacaatcgc cgaaccaatg
    10981 aagccaaaat tctcagcaat gacagtaaaa atcatgtcac tttcacgaac aggaaccgag
    11041 agctctaaaa tattaaaacc tttacctgtt accccgccac ttccaattga taccataccc
    11101 tgtgtttgtt gataagcaat tgagtctgca aaactaaatg gatcaatcca agcagataaa
    11161 cggttgattt gatatgtatc catccccaaa ttataaaacc attccttgcc gtttggtgag
    11221 ataaaaatca tcaaaaagct agctataaaa agaacgatag tagataaaat tggcaagatt
    11281 atccaccaag atatgcccga taaaaggatt aaacctgaca aaatggcaag gaaaactaat
    11341 gctgttccta aatctttttg taacattaga agaatcatca ctggcaatgt aaccaaacca
    11401 aacaatccta gcaacttcca atcatcttga aaagttttgc gatttttctg atgaaaacta
    11461 acagttattc gagataacat taaaatatag gaaatcttca taaactctga cggttgaaac
    11521 aaagttaccg agccaatggt cacccaattt ttagcacctg ttgccgcaaa aagctgtgga
    11581 ctataaaaaa ttaagggaag aaccattagg gttagtccta aagcatacag atagggcgtg
    11641 gctttccata aaaattcagt actgaaaagc ataacgacaa atgcaataat acaccccata
    11701 ataatccaag acacctgctg aaacatcgcg atatagatat tagaaggata atcattcatc
    11761 gttgcaacat aaattgaggc taaaccaatc attaataaaa aaaatactgg tatcacaaca
    11821 gcataatcaa tttgcccttt aaaatagttt gatttttgtg acataggact tcctttttta
    11881 ttcatggtaa ttattataac attttttcag aatctgactt atattttttg caataaaaaa
    11941 tgcaactcaa atcattatga gttgcaaata ttaggagatt gttcttcatc agtaaaacaa
    12001 agaacatgaa aagaaaagtt tatttaggat atttaaagta tacaaagttc accttaaaca
    12061 aaacttaaat aagattattt aagttttgtt attttctcaa cactagtcta aagaataaaa
    12121 tccttcctaa catctcaaaa aaggttttaa tatgtcccct gccaacttct ctaccattaa
    12181 aataggatat attaaaggtt aaaatacacg atacatccat aataaaaagc atattttttt
    12241 attttattct attttaaaat actttttggg gcgaattttg gtccgttatt atgataactt
    12301 cctcagtaca tcaagcactc tctcaactgt aacaacctct tcttctctca cttcttcgtg
    12361 tagtaacaca taatcaaata tctttccgtt tttacgcact atcgctactg tgttccctga
    12421 aatatagcct ttatcaatcg cttctttaaa ctcatctata tctgttctga tagacaaaaa
    12481 gataacaaat aaccgctcgt taaatgagcg gttatttgtt acttcagttt ttctttgaca
    12541 gcatctactg ccccttcaac agcatcttta gcatcatctg ctagttcttt gcctttagca
    12601 attgtttttt cgataaatcc ttttgcttct aactctttat caccggttat tttgccaaag
    12661 ccttctttag caccaccttt tgcttgatct aattttgatt ttagtttttc ttgtgacata
    12721 ctgcacctcc ttattatttt tattaatcat aacatctata tctttcttta gcaaataaaa
    12781 acaagagtcg ctagtgtcag gcgactcttg ttttagtgat tatctcatgg ttatgcgagt
    12841 atggatgtat actataaaaa tagacaacac tttgtgtgtt ataacctgtt ttaaaagaca
    12901 caaaaatctc tgaaggaaga atttaccgtc tgatgaagaa tatggcgctc cctaaaatgg
    12961 ctaccgttaa gacaaaaaca gctcttaaaa agactcaaaa aacgtatcct caaaacttac
    13021 tcaaccagaa atttaatcct gataaaccta atcaagtatg gtctactgac ttcacctata
    13081 tttctattgg atataagaaa tatgtctatc tctgcgcaat aattgatctc tattctagaa
    13141 aatatattgc ttggaaattg agtcatcgta tggatgcaaa gttagcatgt
//
LOCUS       DATRMA010000048        13144 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.048, whole genome shotgun sequence.
ACCESSION   DATRMA010000048 DATRMA010000000
VERSION     DATRMA010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13144
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.048"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            7..480
                     /locus_tag="VBC28_001376"
     CDS             7..480
                     /locus_tag="VBC28_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HEO6815357.1"
                     /translation="MSKKNKIKKTLVDQILDKAKIEHDSLQLDALQGDFPNGIQKQDI
                     FKTLALIGDKTGPIIGILPLTEHLSEKKLAKISGNKKVQMIPQKDLQKITGYIHGANN
                     PIGIRQKHNYPIFIDTIALEKQELIVSAGEIGRSIRINSEVLADFVNAKFADIKE"
     gene            502..1143
                     /locus_tag="VBC28_001377"
     CDS             502..1143
                     /locus_tag="VBC28_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000675283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEO6815358.1"
                     /translation="MKFYLVRHGKTQWNLEGRFQGANGDSPLLEEAIEELEELGQYLS
                     SIHFDAVYSSDLGRARDTVNILNDANSCPKEIHYTPQLREWALGTLEGCKIATMQAIY
                     PRQMTAFYQNPLQFKHDMFGAESLYQTTHRVESFLRSLASKNYDKVLIVGHGANLTAS
                     IRSLLGYQYGSLHYKDKLDNASLTIIETHDFKDFNCLTWNDKSYLRQEVKMTH"
     gene            complement(1179..2081)
                     /locus_tag="VBC28_001378"
     CDS             complement(1179..2081)
                     /locus_tag="VBC28_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEO6815359.1"
                     /translation="MITSIVFDVDDTIYDQQAPYRIAVEKCFPDFDMSAINQAYIRFR
                     HYSDIGFPRVMAGEWTTEYFRFWRCKETLLEFGYREIDEATGIYFQEIYEHELENITM
                     LDEMRMTLDFLKSKNVPMGIITNGPTEHQLKKVKKLGLYDYVDPKRVIVSQATGFQKP
                     EKEIFNLAAEQFDMNPSTTLYVGDSYDNDIMGAFNGGWHSMWFNHRGRSLKPGIKPVY
                     DVAIDNFEQLFGAVKVLFDLPDNKFIFDINDKSNPVLEMGLNNGLMMAAERLLESNMS
                     LDKVVILLRLTAKQEKVLRMKYAR"
     gene            2256..3746
                     /gene="lysS"
                     /locus_tag="VBC28_001379"
     CDS             2256..3746
                     /gene="lysS"
                     /locus_tag="VBC28_001379"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003055423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEO6815360.1"
                     /translation="MSNQHIEELNDQQIVRREKMAALTEQGIDPFGKRFERTATSGQL
                     NEKYADKSKEDLHDIEETATIAGRLMTKRGKGKVGFAHIQDREGQIQIYVRKDSVGEE
                     NYEIFKKADLGDFLGVEGQVMRTDMGELSIKATHITHLSKALRPLPEKFHGLTDIETI
                     YRKRHLDLISNRDSFDRFVTRSKIISEIRRFMDSNGFLEVETPVLHNEAGGASARPFI
                     THHNAQDIDMVLRIATELHLKRLIVGGMERVYEIGRIFRNEGMDATHNPEFTSIEAYQ
                     AYADYQNIMDLTEGIIQHVTKTVKGDGPINYQGTEIKINEPFKRVHMVDAVKEITGID
                     FWKEMTLEEAQALAQEKNVPLEKHFTTVGHIINAFFEEFVEDTLIQPTFVFGHPVEVS
                     PLAKKNDTDPRFTDRFELFIMTKEYANAFTELNDPIDQLSRFEAQASAKELGDDEATG
                     VDYDYVEALEYGMPPTGGLGIGIDRLCMLLTDTTTIRDVLLFPTMK"
     gene            complement(3821..4291)
                     /locus_tag="VBC28_001380"
     CDS             complement(3821..4291)
                     /locus_tag="VBC28_001380"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEO6815361.1"
                     /translation="MTIIEGQLVANEMKIGIVVSRFNELITSKLLSGAVDGLLRHGVS
                     EEDIDIVWVPGAFEIPYMARKMALYKDYDAIICLGVVIKGSTDHYDYVCNEVTKGIGH
                     LNSQSDIPHIFGVLTTDNIEQAIERAGTKAGNKGYDCALSAIEMVNLDKKLRER"
     gene            complement(4306..5499)
                     /locus_tag="VBC28_001381"
     CDS             complement(4306..5499)
                     /locus_tag="VBC28_001381"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HEO6815362.1"
                     /translation="MAFSPIKKLLQDIKSGKMVVLMDDENRENEGDLICAAEMVTKES
                     INFMAKFGKGLICLPLSNYYAEKLELAQMASHNTDNHETAFTISIDHLSTSTGISAED
                     RALTAKMVANDSSKAKDFRRPGHLFPLLAKEGGVLARNGHTEATVDLCRLAGLKECGL
                     CCEIMAEDGSMMRKDELLAFAQKHDLAIATIKQLQDYRRQEEGGVVREIEIQLPTQFG
                     HFTAYGYSEVVANKEHVALVKGDISSGEDVLCRLHSECLTGDVFHSLRCDCGEQLANA
                     LQQIEAEGRGVLLYMRQEGRGIGLINKLKAYHLQEEGLDTLEANLALGFEGDERDYGV
                     SAQLLKDLGINSINLLTNNPDKIQQLEAEGICVKNRVPLQVAVTAYDLNYLKTKKEKM
                     GHLLD"
     gene            complement(5517..6167)
                     /locus_tag="VBC28_001382"
     CDS             complement(5517..6167)
                     /locus_tag="VBC28_001382"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEO6815363.1"
                     /translation="MFTGIIEEMGQVSRIRNGIKSQQLSIDAPKLVPLLRKGDSVAVN
                     GVCLTVLDKSETAFIADVMPESMMRTSLAALRLHSKVNLELALRSDSRLGGHFVLGHV
                     DGVGKIEKIQKDDIAVRFSIDAPPSIMSYIIEKGSVALDGISLTVVSFTEHSFEVSVI
                     PHTMAQTNLSLKKVGDLLNIEVDVLGKYAEKFLAPTNRTNHTSSVMDWSFLSENGY"
     gene            complement(6148..7257)
                     /gene="ribD"
                     /locus_tag="VBC28_001383"
     CDS             complement(6148..7257)
                     /gene="ribD"
                     /locus_tag="VBC28_001383"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEO6815364.1"
                     /translation="MMKKEDYMALALKEAEKGMGFVAPNPLVGAVIVKDDRIISKGYH
                     KRFGDLHAERQAIKNADEDISGSTLYVTLEPCCHVGKQPPCTEALIKSGIKKVVVGSL
                     DPNPLVSGKGIALLRKEGLNVEVGILREECDALNERFIFHMTYKQPFVYLKYAMTLDG
                     KIATKTGDSKWISNEHSRQSVQKLRQKCSAIMVGINTVLADNPRLTCRIPKGEALVRI
                     VCDSQLKIPLDSYLVKSAKTIPTWIATCSDNLAQQQTLKEMGCRLIKVPRKDGKLDLK
                     VLMTILGQEGIDSLLIEGGSSLHFSALKAGIVNRLIVFIAPKIIGGLKAKTAISGEGL
                     DWLNQAFRVKDIELSRMDSDVVIEGKVEHYVYRNY"
     regulatory      complement(7451..7565)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            7986..9326
                     /locus_tag="VBC28_001384"
     CDS             7986..9326
                     /locus_tag="VBC28_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001073016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HEO6815365.1"
                     /translation="MNQNHSVKRSLSEVNQSVEVPHNSSFWNTLRAFLGPGALVAVGY
                     MDPGNWITSVIGGATYRYLLLFVVLVSSLMAMQLQQMAGKLGIVTRQDLAQATASRLP
                     KPLRYLLFIIIELALIATDLAEVIGSAIALHLLFGWPLLLSIMITILDVFLLLLLMKL
                     GVQKIEAFVSVLILTILIIFTYLVVLSQPDLDAMFKGFLPHHELFNISHEGKNSPLTL
                     ALGIIGATVMPHNLYLHSSLSQTRRVDYHNKSSIKKAVRFMTLDSNIQLSLAFVVNSL
                     LLVLGASLFYGHANDISAFSQMYLALSDKTITGAVASSFLSTLFAVALLASGQNSTIT
                     GTLTGQIVMEGFLHFKLPQWLIRLCTRLLTLLPIFVIALLVGGEENTLDQLIVYSQVF
                     LSLALPFSIFPLIYFTSQKSIMGEHANAKWNTYLAYLVAIILTLLNLKLIMDLL"
     gene            complement(9428..10225)
                     /locus_tag="VBC28_001385"
     CDS             complement(9428..10225)
                     /locus_tag="VBC28_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000618018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6815366.1"
                     /translation="MIRHSFKSKVLLLVFILTIIVNYLSATGFLTGNSQKSLSDRYQT
                     LLTPAPLAFSIWSVIYLLTFLVILRAIFSKSQSYQDNFASIFPYFLGLLLVNNIWTVF
                     FTSNLIGLSTIIIFAYCILLVIIIKILSKNKSKLLLRITFGIHAGWLLVASLVNLAVY
                     LVKIDFNYPLPKVYIAIIALIFITVLSLYLARVLQNAYLILSVFWAWLMVFKAHLEGN
                     FQHLYFSIQILSLIAAIILGVSFFIIGYYNVKERYKQKNTSPYSRVL"
     gene            complement(10349..10561)
                     /locus_tag="VBC28_001386"
     CDS             complement(10349..10561)
                     /locus_tag="VBC28_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HEO6815367.1"
                     /translation="MSEFTFEIVEKLLVLSENEKGWTKELNRVSFNGAPAKFDLRTWS
                     PDHTKMGKGITLSNEEFKVILDAFRK"
     gene            complement(10688..11974)
                     /locus_tag="VBC28_001387"
     CDS             complement(10688..11974)
                     /locus_tag="VBC28_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEO6815368.1"
                     /translation="MSNVKKRPEVLSPAGTLEKLKVAIDYGADAVFVGGQAYGLRSRA
                     GNFSMEELQEGINYAHARDAKVYVAANMVTHEGNELGAGPWFRELRDMGLDAVIVSDP
                     ALIVICATEAPGLEIHLSTQASSTNYETFEFWKEMGLTRVVLAREVTMAELAEIRKRT
                     DVEIEAFVHGAMCISYSGRCVLSNHMSHRDANRGGCSQSCRWKYDLYDMPFGQERQSL
                     KGEIPEPFSMSAVDMCMIEHIPDMIENGVDSLKIEGRMKSIHYVSTVTNCYKAAVDAY
                     MESPEAFEAIKEDLIDELWKVAQRELATGFYYHTPTENEQLFGARRKIPQYKFVGEVV
                     SFDNAKMEATIRQRNVIMEGDRVEFYGPGFRHFECFIDGLRDAEGNKIDRAPNPMELL
                     TITLPNPVKKGDMIRACKEGLVNLYQNDGTSKTVRA"
     gene            complement(12104..13030)
                     /locus_tag="VBC28_001388"
     CDS             complement(12104..13030)
                     /locus_tag="VBC28_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012997850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEO6815369.1"
                     /translation="MEKIILTATAESIEQVKQLLAIGIDRIYVGEENYGLRLPHSFSD
                     DELREIAKLVHDAGKELTVACNALMHQEMMDNIKPFLELMKEINVDYLVVGDAGVFYI
                     NKRDGYNFKLIYDTSVFVTSSRQVNFWGQHGAVETVLAREIPSEELFKMSENLEFPAE
                     ILVYGASVIHHSKRPLLQNYYNFTHITDEKTRERGLFLAEPGDPESHYSIYEDKHGTH
                     IFINNDINMMTKVTELVEHHFTHWKLDGIYCPGDNFVAIAEIFVETARLIENGTFTQD
                     QAFLFDERIRKLHPKGRGLDTGFYDFDPSTVK"
BASE COUNT         4214 a         2633 c         1996 g         4301 t
ORIGIN      
        1 gtcattatga gtaaaaagaa taaaatcaaa aaaactttag ttgatcaaat tttagataaa
       61 gcaaaaatcg aacatgatag tctacaattg gatgctttac agggagattt ccccaacggt
      121 attcaaaaac aagatatctt taagacatta gcgctaatcg gtgataaaac cggtcccata
      181 ataggtatct tacctctcac cgagcattta tccgaaaaaa agttagctaa aatttcagga
      241 aacaaaaagg tacaaatgat acctcaaaaa gatctccaga aaataaccgg ttatattcac
      301 ggagcaaata atccaattgg aattcgccaa aaacataatt accctatttt tatcgatact
      361 atcgctttag aaaaacaaga actcattgtc tctgcaggag aaattggacg ttctattcgt
      421 attaatagtg aggttcttgc agatttcgta aatgctaaat ttgccgatat aaaggaataa
      481 taattaggat tggagaaaaa tatgaaattt tatcttgtta gacacggaaa aacacaatgg
      541 aatttagagg ggcgtttcca aggtgctaac ggagactcac ctctccttga agaagctatt
      601 gaagaattag aagaacttgg acaataccta tcatctattc attttgatgc tgtttattct
      661 agcgacttag gtcgtgctag agatactgtt aatatcctca atgatgctaa ttcatgccca
      721 aaagaaatcc actatactcc tcaacttcgt gaatgggcat tgggaacttt agaaggttgt
      781 aaaattgcaa ctatgcaagc tatctatcct cgtcaaatga ccgcatttta tcaaaatccc
      841 ttacaattta aacatgacat gttcggtgca gaatctcttt atcagacaac gcatcgtgta
      901 gagagtttct tacgttctct agcttcaaaa aattacgaca aggtccttat tgtaggacat
      961 ggagcaaacc taactgcgtc tattcgttcc ttactgggtt atcaatatgg tagtttacac
     1021 tacaaagata aacttgataa tgccagcctc acaatcatag aaactcatga ttttaaagac
     1081 ttcaactgtc tgacttggaa cgataaatct tatttaaggc aagaggtcaa aatgacacac
     1141 taaaataatc cactagattc atctagtgga ttatttaatt atctagcata cttcatgcgt
     1201 aatacttttt cttgttttgc agttaggcgc agtaaaataa caactttgtc aaggctcata
     1261 ttactctcaa gcagacgctc tgctgccatc attaaaccat tattaagtcc catttcaagg
     1321 actggattac ttttatcgtt gatatcaaaa ataaatttat tatcaggtaa gtcaaacaac
     1381 actttaacag caccgaataa ttgctcaaag ttatcaatag caacatcata aactggttta
     1441 attcccggtt ttaaagaacg tcctctatgg ttgaaccaca tagaatgcca accaccatta
     1501 aatgcaccca taatatcatt atcatatgaa tcacccacat aaagtgtagt tgaagggttc
     1561 atatcaaatt gctctgctgc taaattaaaa atctcctttt caggcttttg aaaaccagtt
     1621 gcttgactaa ctataacacg ttttggatca acatagtcat aaagtcccaa ttttttaact
     1681 tttttcaatt gatgttccgt aggaccattt gtaataattc ccattggtac attttttgat
     1741 ttcaaaaagt caagtgtcat acgcatctca tcaagcattg taatattttc aagttcatgt
     1801 tcgtatattt cttgaaaata aataccagta gcttcgtcaa tttcacgata accaaactct
     1861 aaaagcgttt ctttgcatcg ccagaaacga aaatattctg ttgtccattc accagccatt
     1921 actcgtggaa agccaatatc tgaataatga cgaaagcgaa tataagcttg gttaatagcg
     1981 ctcatatcaa aatcaggaaa gcatttctcc acagcaatac ggtaaggtgc ttgttgatcg
     2041 taaatcgtat catcaacatc aaaaacaatt gaagtaatca tgattatcct tttctttctg
     2101 aattttcaat tgttaattat acctttttct acccttattt tcaaatggtg aaaagtctat
     2161 taaaaatgag aataacacca gaaactcata aaataaatta tgaattatca gtgtttttgt
     2221 ggtacaatag gatgaatact aacttggagg aaattatgtc taaccaacat attgaagaat
     2281 taaatgacca acagattgtt cgtcgtgaaa aaatggctgc tttgactgaa caaggcattg
     2341 atccttttgg taaacgattt gaacgtacag caacatctgg tcaattaaat gaaaaatacg
     2401 ctgacaaatc aaaagaagac ttgcacgaca ttgaagaaac tgctacaatc gctggtcgtt
     2461 taatgactaa gcgcggtaag gggaaagttg gctttgcgca tatccaagat cgcgaaggtc
     2521 aaattcaaat ttatgtccgt aaagattctg ttggcgaaga aaactacgaa atctttaaaa
     2581 aagctgattt aggtgacttc cttggtgttg aagggcaagt aatgcgtaca gatatgggag
     2641 aactttctat caaggctact catattactc acctttcaaa agcactccgt ccacttcctg
     2701 aaaaattcca cggcctaact gatattgaaa caatctatcg taaacgtcat cttgatttaa
     2761 tctcaaatcg tgacagcttt gaccgttttg taacacgctc aaaaatcatt tcagaaattc
     2821 gtcgctttat ggatagcaat ggtttcttag aggttgaaac acctgttctt cataatgaag
     2881 ctggtggtgc ttctgctcgc ccatttatca cacatcacaa tgcacaagac attgacatgg
     2941 ttcttcgtat cgcaactgaa ttgcatctaa aacgtcttat tgtaggtggt atggagcgtg
     3001 tttatgaaat cggacgtatt ttccgtaatg aagggatgga tgcaacccac aatccagagt
     3061 tcacatctat tgaagcctac caagcctatg ctgactacca gaatattatg gacttgacag
     3121 aaggtattat ccagcatgtt acaaaaactg taaaaggtga tggaccaatt aattatcaag
     3181 gcacagaaat taaaattaat gaaccattca aacgtgttca tatggttgat gcggttaaag
     3241 aaatcactgg tattgacttc tggaaagaaa tgactcttga agaagcacaa gctttggcac
     3301 aagaaaaaaa tgttccgctt gaaaaacatt tcacaacagt tggtcacatc attaatgcct
     3361 tcttcgaaga atttgttgaa gacactttaa ttcaaccaac ttttgtcttt ggtcatccag
     3421 ttgaagtgtc tccacttgct aagaaaaatg atacagaccc tcgttttaca gaccgtttcg
     3481 aactctttat catgactaaa gaatacgcta atgcttttac agaattaaat gacccaattg
     3541 atcagctatc tcgctttgaa gcacaagcat ctgctaaaga acttggcgat gatgaagcaa
     3601 ctggagttga ttacgattat gttgaagcac ttgaatatgg tatgccacct actggtggac
     3661 taggtattgg tatcgaccgt ctctgtatgc tgttaactga tacaacaact atccgagacg
     3721 tcctactttt ccctacaatg aaataagaat atattaaaac ccatccttat ttaaggtggg
     3781 ttttaatatt acctctactg aactttgtta ggtttctgat ttatcgctca cgcaattttt
     3841 tatcaaggtt caccatttca atagccgaaa gagcacaatc ataaccttta tttcctgctt
     3901 ttgttcctgc cctctcaata gcttgttcga tgttatccgt tgttagaaca ccaaagatat
     3961 gagggatatc tgactggcta tttaaatgac caataccttt tgtcacctca ttacaaacat
     4021 aatcataatg atcagtgctg cctttaatga ctaccccaag acagataata gcatcataat
     4081 ctttatataa tgccattttt ctagccatat aggggatttc aaaagcacca ggtacccata
     4141 caatatctat atcttcttct gaaacaccat gtctcagcag tccatctact gctcctgata
     4201 ataatttaga tgtaatcaat tcattgaaac gtgagactac gattcctatt ttcatttcgt
     4261 ttgccactaa ttgtccttca ataattgtca ttttttcttc ctcctttaat ctaaaagatg
     4321 tcccattttt tcttttttag tttttaaata attaaggtca taagctgtta ctgccacctg
     4381 taatggcact ctgtttttga cgcaaattcc ttctgcttca agttgttgga ttttatctgg
     4441 attattggtt agtaagttta tagaatttat acctaaatct tttaaaagtt gagcactaac
     4501 tccatagtct ctctcatctc cttcaaaacc taaagccaaa ttagcttcta aagtatctaa
     4561 gccttcttct tgcaaatgat aagcttttaa cttattaatc aaaccaattc ctcgaccttc
     4621 ctgtctcata taaagaagga ctccacgacc ttcagcctct atttgttgga gggcattagc
     4681 cagttgttcc ccacaatcac atctcaagga atgaaagaca tcacctgtca aacattctga
     4741 gtgtaaacga cataaaacat cttcaccact actaatatcc cctttaacaa gggcaacatg
     4801 ttccttattt gccacaactt cgctatatcc ataagccgta aaatgaccga attgcgttgg
     4861 tagctgaatc tcaatttctc taaccacccc tccctcttct tgacgtctat agtcttgcaa
     4921 ttgttttatg gtagcgatag ctaaatcgtg cttttgagca aatgctaata actcatcctt
     4981 tctcatcata ctaccatctt cagccattat ttcacaacaa agtccacatt cctttaatcc
     5041 agctaaacga catagatcaa cggttgcttc agtatgtcca tttctagcta aaacacctcc
     5101 ttccttggct aacaagggaa ataagtgccc aggacgtcta aagtctttag ctttgctact
     5161 gtcgttagca accatcttag ctgtcaaagc tctatcttcc gcagaaatcc cagtactagt
     5221 tgatagatga tcaatagata tggtaaaagc tgtttcatga ttatcagtat tatggctagc
     5281 catttgagct aattctaact tttcagcata gtaattagac agaggtaagc aaattaatcc
     5341 tttcccaaat ttagccataa aattaatcga ctcttttgtc accatttcag ccgcacaaat
     5401 aaggtctcct tcattctctc tattttcgtc atccatcaac actaccattt tccctgattt
     5461 gatgtcttgt aaaagttttt ttattggact aaaggccatt agctttctcc tctctcttag
     5521 tagccatttt ctgataaaaa agaccaatcc attaccgatg aagtgtgatt agttctatta
     5581 gtgggtgcta aaaatttctc agcatatttg cctaaaacat ccacttcgat atttaataaa
     5641 tccccaacct tttttaagga tagattagtt tgagccatcg tgtggggaat gaccgaaact
     5701 tcaaaagaat gctctgtaaa tgataccact gttaagctaa tcccatctag agccaccgaa
     5761 cctttctcta taatataaga cataatacta ggtggagcat caattgaaaa tctgacagca
     5821 atatcatctt tttggatttt ttctattttt ccaaccccat ccacatgtcc aagaacaaaa
     5881 tgccccccta accttgagtc acttctgagt gctaattcta agttaacttt agaatgaagt
     5941 cgtaaagcag ctaagcttgt tcgcatcata ctttctggca tgacgtctgc tataaaagct
     6001 gtttcacttt tgtcaagaac cgtcaaacaa acaccattaa cagctacgct atcccctttt
     6061 cttaataaag ggactagttt gggagcatca atacttaatt gttgggactt tatgccattc
     6121 cgaatacgac tgacttgacc catttcctca ataattcctg taaacataat gttctacctt
     6181 tccttcaata acaacgtcag aatccatacg actaagttca atatctttta ctctgaaagc
     6241 ttggttaagc caatccaaac cttctccaga aatagctgtt tttgctttca gacctcctat
     6301 tattttgggt gcaataaaaa caattagtct gttaacaata ccagctttta aagctgaaaa
     6361 atgtaaacta cttcctcctt caataagaag gctatcaatc ccttcttgtc ctaaaatagt
     6421 cattaatact ttcaaatcca gctttccatc ttttcttggg accttaatca aacggcaacc
     6481 catttctttt aatgtttgtt gctgagcaag attatctgaa catgttgcaa tccatgttgg
     6541 tattgtttta gcgctcttaa ctaggtaaga gtctaaaggt atcttcaact gactatcaca
     6601 tacgatcctc acaagcgctt cacctttagg aatacggcaa gttaaacgtg gattatcagc
     6661 taatacagta ttgataccga ccataatagc actacatttt tgcctcaatt tctgaacaga
     6721 ttgtcttgag tgctcatttg atatccactt agaatcgccc gtctttgtag ctatcttccc
     6781 atctaaagtc atagcatatt tcaaataaac aaaaggctgc ttataggtca tgtgaaaaat
     6841 aaatctttca ttcagagcat cgcattcttc tcttaagata ccaacttcaa catttaaacc
     6901 ttcttttctc aacaaggcaa tcccttttcc tgaaactaaa ggatttggat ctaaacttcc
     6961 aactactact tttttgatac cagatttaat aagagcttca gtacaaggtg gttgcttacc
     7021 aacatgacag catggttcta aagtcacgta cagagtactt ccagagatat cctcatcagc
     7081 atttttaata gcctgtcttt ccgcatgtaa atctccaaat cgtttatgat aacctttgga
     7141 aattatacga tcatctttaa caataacagc acctactaat gggttgggag caacgaaccc
     7201 catacctttc tcagcctctt tcaaggctaa tgccatgtag tcttcttttt tcatcatttc
     7261 ctttttcctt ctaaaataaa aacccttagc ttctaaaata gaaaactaag gggagggatg
     7321 agaagtcgtt gattatacta attagcatta tattttaggt agcacaaaaa aactaaagac
     7381 tccctataga aaactatagg gagtctctca ttaatccagc acaaataaac tgcaagtagc
     7441 agacaatcac tgaattcttc tctcatccag actttactgt cggtactgga atcacaccag
     7501 ttcatgctcg aaagctcgcg gactataccg ccggttagga ttttcacctg accccgaaga
     7561 aattctattt gacagttatt ttactaaact catttttatt tgtcaataaa aataatgtgt
     7621 tttttattat aaaataataa ttaagtagct catctattct tatctctaca agacagtata
     7681 tctataccat aaagcaatct ttcgaactaa tcaaacaaga atcgctagta ttattggatt
     7741 atatttctta aaaaattcac tacagatata gtttactcca cttaaataac ttaggtaaat
     7801 ctacggaact aaaaataatc gatagaaatc tgttattttg ttatttcaaa tgtaactgtt
     7861 aagaaaataa ttctctaaat actaaacagc acctcaattg attattaagt agcgttaatt
     7921 tttaattgtt tttctatttt ctatttgtta taatggcttg agattactag aaagtatagt
     7981 attacatgaa tcaaaatcat tccgtcaaaa gatccttatc cgaagttaat cagagcgttg
     8041 aggttcctca caatagctct ttttggaaca ctttaagagc ttttcttgga cctggagctt
     8101 tggttgcagt tggttatatg gatccaggta actggattac tagcgttatt ggtggagcta
     8161 cctatcgtta cttactattg tttgttgttt tagtatcttc tttgatggcg atgcaattac
     8221 aacaaatggc tggcaagctt ggcattgtta cacgacagga cttagcacaa gcaacagctt
     8281 ctcgactccc aaaaccgctt cgttacctgc tatttattat cattgagtta gcccttatcg
     8341 ctactgattt agcagaagtc attggctctg ccattgctct tcacttatta tttggctggc
     8401 ccctgttact gtctatcatg attactattt tggatgtatt cttactttta ctcttaatga
     8461 agttaggcgt ccaaaaaatt gaagcctttg tttctgttct catattgaca attttgataa
     8521 tctttacata tttagttgtt ttatcacaac ctgatttaga tgctatgttc aaaggatttt
     8581 taccacacca tgagcttttt aacatatcac atgaagggaa aaatagtcct ttaaccctcg
     8641 ctcttggcat tatcggcgca acagtcatgc cacataatct ttatttacat tcttctttat
     8701 ctcagactag acgcgtggat tatcataata aatcttcaat aaaaaaagct gtacgtttca
     8761 tgaccttaga ttctaacatt cagctatcac ttgcatttgt cgtgaattcg ctactattag
     8821 tcttaggagc tagtctattt tatgggcatg ctaatgatat cagtgccttt tctcaaatgt
     8881 atcttgccct atctgataaa actattactg gtgcagtagc cagttctttc ctttctaccc
     8941 tttttgcagt tgccctacta gcaagtggtc aaaattcaac gattacaggt actttaacag
     9001 gacaaatcgt tatggaaggt ttccttcact tcaaattacc tcaatggtta ataaggcttt
     9061 gtactagatt attaactctt cttccaattt ttgttatcgc cctactagtt ggaggcgaag
     9121 aaaatacgtt agatcaacta atcgtctatt cacaagtttt tctttcctta gctttaccat
     9181 tttcaatctt tccattgatt tatttcacgt cgcaaaaatc aattatggga gagcatgcta
     9241 atgctaaatg gaatacctat ttagcatacc ttgtcgccat cattttaaca ttactcaatt
     9301 tgaaattaat catggattta ctttaataca atacaaaaaa gccccactaa tgaactgaac
     9361 ctctatcatg agacatacat ctaatgatag aggaaagtac ataatgatgg agctttttgt
     9421 atttctttca aagaacccgt gagtaagggc tagtattttt ttgcttgtac ctctccttga
     9481 cattataata accgatgatg aaaaacgata cgccaaggat aattgctgca atcaaagata
     9541 aaatttggat actaaaatat aggtgttgaa aatttccttc taaatgtgct ttaaaaacca
     9601 tcaaccaagc ccaaaaaacg gataagatta agtaagcatt ttgtaatact ctagctaggt
     9661 aaagacttaa aacagttata aaaatcaagg caatgatagc tatatatacc tttggtaatg
     9721 ggtaattaaa atcaatttta actaagtaga ccgctaaatt gaccaatgac gcaactagca
     9781 accatcctgc atgaattcca aatgttattc taagcagtaa ttttgattta tttttactta
     9841 gtatcttgat aatgataacc agaagaatac agtaagcaaa aattattatt gttgataggc
     9901 caatcaaatt actcgtaaaa aatacagtcc atatattatt aactaataga agacccaaaa
     9961 agtatggaaa aatgctagca aagttatctt ggtaagattg actcttactg aatatagctc
    10021 ttaatattac taaaaaggtc aataaataga tgactgacca aattgaaaag gctaatggtg
    10081 ctggtgtcaa gagcgtttga taacgatcag acagactctt ctgactgttc cctgttaaaa
    10141 aacctgttgc ggataaataa ttcactatta ttgttaagat aaagactaac aataacacct
    10201 tacttttaaa tgaatgtcta atcatagagg cctcctttta cctccattat aattctttta
    10261 gtttaaaaat tctatttttt taatcaatat gtttactgaa tactcgtttg tctcgacaaa
    10321 aaaagtggtt tttgaaacca ctttttattt atttacgaaa agcatctaaa atgactttaa
    10381 attcttcatt agataaagtg atacctttac ccatttttgt atgatctgga ctccatgtac
    10441 gcaaatcaaa tttagctggc gctccgttga agctcacacg attcaactct tttgtccaac
    10501 ctttttcatt ttcagatagg accaataact tctcaacaat ttcaaatgta aattctgaca
    10561 ttttatacct ccacttatac tattcgttat ttttatagct ttttgttcta tttaaatgaa
    10621 cttcttctat tttaacaatt tttagtaata ttgaccatgt aaaatgacaa aatagttata
    10681 aaagtatcta agctcttaca gtcttgctag taccatcatt ttgataaagg ttcactaatc
    10741 cttctttaca agcacgaatc atatcccctt tttttactgg atttggtaat gttatggtta
    10801 ataattccat cggatttgga gcacggtcta ttttatttcc ttcagcatca cgcagaccat
    10861 caataaaaca ttcaaagtga cggaagccag gaccatagaa ttctacgcga tctccttcca
    10921 taataacatt acgctgacga attgtagcct ccattttagc attgtcaaat gaaaccactt
    10981 ccccaacaaa tttgtattga ggaattttac gacgagctcc aaagagttgt tcattttcag
    11041 ttggtgtatg gtagtagaaa cctgttgcta attcgcgttg tgcaaccttc caaagttcat
    11101 caatcaagtc ttctttaata gcttcaaaag cttctggact ttccatatag gcatctacag
    11161 cagctttata gcaattagtt actgtagaaa cataatgaat ggatttcata cgtccttcta
    11221 tttttaaact atctacacca ttttcaatca tatctggaat atgctcaatc atacacatat
    11281 ccacagctga cattgagaaa ggttctggaa tctcgccttt taacgattga cgttcttgtc
    11341 caaatggcat atcgtagagg tcatatttcc aacgacatga ctgagagcaa ccgccacgat
    11401 tagcatcacg atggctcata tggtttgata gaacacatcg tcctgagtat gaaatacaca
    11461 tcgcgccatg aacaaatgct tctatctcaa catctgtcct cttcctgatt tcagccaact
    11521 ctgccatagt aacctcacgt gctaatacga cacgagtaag ccccatctct ttccaaaatt
    11581 caaaagtttc atagttcgtg gaagaggctt gagttgacaa atgaatttct aagcctggtg
    11641 cttctgtagc acaaataaca ataagagcag gatctgaaac aatgactgca tctagtccca
    11701 tatcacgtaa ttcacgaaac cacggacctg ccccaagctc attaccttca tgagtaacca
    11761 tattagccgc tacataaact ttagcatctc ttgcatgagc atagtttatt ccctcttgta
    11821 actcttccat agaaaagtta ccagctctac ttcgaagacc atacgcttga cctccaacaa
    11881 atacagcatc tgctccataa tcaatagcaa cttttaattt ttctagtgtt cctgcaggtg
    11941 ataaaacctc agggcgtttt tttacattag acatcttata tttccttcta ttttcaagat
    12001 aaaagaatta ttttctatct taattaacat accaatcgat atgcagttct tagtgtaatt
    12061 ggctacgctt tgaaatagaa aactttcacg tagcaaataa atcttatttt actgttgatg
    12121 ggtcaaaatc ataaaatcca gtatctaaac cacgtccttt agggtgcagt tttctaattc
    12181 gttcatcaaa caagaaagcc tgatcctgag tgaatgttcc attttcaatc aaacgagctg
    12241 tttcgacaaa aatttcagca atagcaacaa aattatctcc tggacaataa ataccatcta
    12301 atttccagtg agtaaagtga tgttcaacta gttcagtcac tttagtcatc atattgatat
    12361 cattgttaat aaaaatatga gtcccatgct tatcttcgta aatagaatag tgactttctg
    12421 gatcacctgg ttctgctaaa aaaagaccac gctcacgtgt tttttcatca gtgatatgag
    12481 taaaattata atagttttgc aataacggac gcttagagtg atgaattaca gaagccccat
    12541 aaactaaaat ctcagcagga aattcaagat tctcagacat cttaaaaagt tcttcagaag
    12601 gtatttcacg tgctaaaacc gtttctactg ctccatgttg tccccaaaaa ttaacttgac
    12661 gacttgatgt aacgaagaca gaagtgtcgt agataagttt gaagttataa ccatcacgct
    12721 tgttgatata aaatactcct gcatcaccaa ctaccaaata atccacatta atttctttca
    12781 tcaactccaa aaatggtttg atattatcca tcatctcttg atgcatgagt gcattacaag
    12841 ctactgtcaa ttctttacca gcatcatgta ctaatttagc gatttcacgc aattcatcgt
    12901 ctgaaaatga atgtggtaaa cgaagaccgt aattttcttc ccctacatag atgcggtcaa
    12961 ttcctattgc tagcaattgc tttacttgtt caatagattc agctgtcgct gtcaaaatta
    13021 ttttttccat aattagtatt ataacaaact ttcagcattt ttaagcatat ttcttgacaa
    13081 gaataaataa aaatattctt ttcgaaaatt atatcacttt agaggttaag cactatcaga
    13141 taag
//
LOCUS       DATRMA010000049        13003 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.049, whole genome shotgun sequence.
ACCESSION   DATRMA010000049 DATRMA010000000
VERSION     DATRMA010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13003
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.049"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            24..1094
                     /gene="xerS"
                     /locus_tag="VBC28_001389"
     CDS             24..1094
                     /gene="xerS"
                     /locus_tag="VBC28_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HEO6815370.1"
                     /translation="MKRELLLEKIDELKEIMPWYVLEYYQSKLSVPYSFTTLYEYLKE
                     YRRFLEWLLDSGVANCHHIAEIELSVLENLTKKDMEAFILYLRERPLLNANTRQNGVS
                     QTTINRTLSALSSLFKYLTEEVENADGEPYFYRNVMKKVSTKKKKETLASRAENIKQK
                     LFLGNETIEFLEYIDCEYQNKLSKRALAFFNKNKERDLAIIGLLLASGVRLSEAVNLD
                     LKDINLNVMVIDVTRKGGKRDSVNVASFAKPYLANYLDIRKNRYKAENQDIALFLSEY
                     RGVPNRIDASSVEKMVAKYSQDFKVRVTPHKLRHTLATRLYDATKSQVLVSHQLGHAS
                     TQVTDLYTHIVNDEQKNALDKL"
     gene            complement(1137..2075)
                     /locus_tag="VBC28_001390"
     CDS             complement(1137..2075)
                     /locus_tag="VBC28_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEO6815371.1"
                     /translation="MIRQFLREHLIWYILYIMMFVLFFISFYLYHLPMPYLFNSLGLN
                     VIVLLGISIWQYSRYRKKMLHLKYFNSSQDPSFELQPSDYAYFNIITQLEAREAQKVS
                     ETIEQTNHVALMIKMWSHQMKVPLAAISLMAQTNHLDPKEVEQQLLKLQHYLETLLAF
                     LKFRQYRDDFRFEAVSLREVVVEIIKSYKVICLSKSLSIIIEGDNIWKTDKKWLTFAL
                     SQVLDNAIKYSNPESKIIISIGEESIRIQDYGIGILEEDIPRLFEDGFTGYNGHEHQK
                     ATGMGLYMTKEVLSSLNLSISVDSKINYGTAVSIHK"
     gene            complement(2072..2740)
                     /locus_tag="VBC28_001391"
     CDS             complement(2072..2740)
                     /locus_tag="VBC28_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO6815372.1"
                     /translation="MSQEQGKIYIVEDDMTIVSLLKDHLSASYHVSSVSNFRDVKQEI
                     IAFQPDLILMDITLPYFNGFYWTAELRKFLTIPIIFISSSNDEMDMVMALNMGGDDFI
                     SKPFSLAVLDAKLTAILRRSQQFIQQELTFGGFTLTREGLLSSQDKEVILSPTENKIL
                     SILLMHPKQVVSKESLLEKLWENDSFIDQNTLNVNMTRLRKKIVPIGFDYIHTVRGVG
                     YLLQ"
     gene            complement(2850..4805)
                     /locus_tag="VBC28_001392"
     CDS             complement(2850..4805)
                     /locus_tag="VBC28_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6815373.1"
                     /translation="MFYLKIAWHNLKHSIDQYIPFLLASLLLYSLTCSTLLILMSAVG
                     RDMGTAATVLFLGVIVLSIFAVVMEHYSYNILMKQRSSEFGLYNILGMNKRQVARVAS
                     LELFIIYIFLISIGSLFSAFFAKFIYLIFVNIINYHALNLSLSLWPFIICIVIFTGIF
                     LTLEVPVIRHVHLSSPLSLFRKKQQGEKEPKGNLILAILALVAIAIAYTMALTSGKAP
                     ALAVIYRFFFAVLLVIAGTYLFYISFMTWYLKRLRQNKHYYYKSEHFVSTSQMIFRMK
                     QNAVGLASITLLAVMALVTIATTVSLYSNTQNVVTGLFPKSVSLSIDNSKGDAKNIFE
                     EKILKKLGKSSKEAITYNQTMISMPVSQSSELNITSKNVKHVDITKTGFMYLITQNDF
                     RRLGHQLPKLKDNQVAYFVQKGDSRLKKINLLGNKFDVVKNLKEAYVPETTNTYNPGL
                     IIFANNKQIDNIRKAYLPYTKNINTFPKTFKAYLDLNSQEINSISKNDIIEVDGKYVG
                     NISTKQSFLKEGYQMFGGLLFTGFLLGISFLLGIALIVYYKQYSEGHEDKRSYRILQE
                     VGMSKKLVKRTINSQIMIFFFQPLVVAVIHFGVAIPMLKQMLLVFGVLNSTIVYVVSG
                     LTVLAISIIYFIIYRITSRTYYHIIER"
     gene            complement(4807..5559)
                     /locus_tag="VBC28_001393"
     CDS             complement(4807..5559)
                     /locus_tag="VBC28_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6815374.1"
                     /translation="MLLEINHLEKVFRTRFSKEETRALQDVDFKVEQGEFIAIMGESG
                     SGKTTLLNILATLEKPTNGQVILNGEDITKIKEAKLASFRLKNLGFVFQDFNLLDTLS
                     VRDNIYLPLVLDRKRYKEMDHRLSELSSHLRIDDLLDKRPFELSGGQKQRVAIARSLI
                     TNPQILLADEPTAALDYRNSEDLLNLFETINLDGQTILMVTHSANAASHAKRVLFIKD
                     GRIFHQLYRGNKNNSEFNKDISLTMSAILGGE"
     gene            complement(5585..6547)
                     /locus_tag="VBC28_001394"
     CDS             complement(5585..6547)
                     /locus_tag="VBC28_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001253131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEO6815375.1"
                     /translation="MRRKIVLLFVVPMLIVLGILGVVVHYYGSALNIYLLPPSSERYG
                     RVILDRVEQRGLYSQGRQWQIIRQRSEKKLKTSKSYQESRNIVQEAVRYGGGKHSQIL
                     SKETVRRDTLDSRYPEYRRLNEDILLITIPSISKLDKRSISHYSGKLQNILMEKSYKG
                     LILDLSNNTGGNMIPMIGGVASILPNDTLFHYTDKYGNKKTITMKNIPLEALKISRKT
                     INTKHVPIAIITNHKTASSAEMTFLSFKGLPNVKSFGQATAGYTTVNETFMLYDGARL
                     ALTTGIVSDRQGYKYENTPILPDQVTSLPLQESQSWLKSRINQN"
     gene            6713..7202
                     /locus_tag="VBC28_001395"
                     /pseudo
     CDS             6713..7202
                     /locus_tag="VBC28_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140790.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
     gene            complement(7234..8082)
                     /locus_tag="VBC28_001396"
     CDS             complement(7234..8082)
                     /locus_tag="VBC28_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEO6815376.1"
                     /translation="MKIKKLLGLTTTVVISALILGACGQSKNEDAKVVRVGTMVKSKT
                     EKARWDKIEELVKKKGVKLKFTEFTDYTQPNKALESDEIDINAFQHYNYLNNWNKANK
                     TNLVSVAETYFTSFRLYSGTKNGKGKYQTVSEIPNKATITIPNDAVNESRSLYLLQSA
                     GLLKLKVSGDALATMSDVVSNPKSLDLKEVDAAQTARSLDSTDAAVINNDFVTEAGIN
                     PKSAIFIEPKSKNAKQWYNLLVAQKGWQDKSKAKAIKEVVKAYHTDAVKKVIEKTSQG
                     LDQPVW"
     gene            complement(8215..8958)
                     /locus_tag="VBC28_001397"
     CDS             complement(8215..8958)
                     /locus_tag="VBC28_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000559006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO6815377.1"
                     /translation="MHYKELLESSEKAYLETNRYKHFKNIIISERYFSNYISYKIMPT
                     VSEFLQDMIYLADEQKDYQSDFAFVFFEENAELSSELQRTLSQQGFESEKHIIFTNSL
                     NHLKLFEKEFDDITFTKLNENYLEKYLKFKYKQSIVYGEIYAKQMYNDNRVNLLNNNA
                     KVYLAIKDNQIIGDVTAWDYGEYVEIDDFYVQESYRGQGIGTRLQFDASRGFKKVILI
                     SEEENRKMYEHQGYEEVAYYWTALRSPRK"
     gene            complement(9036..10370)
                     /gene="trmFO"
                     /locus_tag="VBC28_001398"
     CDS             complement(9036..10370)
                     /gene="trmFO"
                     /locus_tag="VBC28_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HEO6815378.1"
                     /translation="MSQSYINVIGAGLAGSEAAYQIAKRGIPVKLYEMRGVKSTPQHK
                     TDNFAELVCSNSFRGDSLTNAVGLLKEEMRRLDSIIMRNGEAHRVPAGGAMAVDREGY
                     SEAVTEEIHKHPLIEVIRDEITDIPGDAITVIATGPLTSDSLAAKIHELNGGDGFYFY
                     DAAAPIVDKNTIDINKVYLKSRYDKGEAAYLNCPMTKEEFMAFHEALTTAEEAPLNSF
                     EKEKYFEGCMPIEVMAKRGIKTMLYGPMKPVGLEYPEDYKGPRDGEFKTPYAVVQLRQ
                     DNAAGSLYNIVGFQTHLKWGEQKRVFQMIPGLENAEFVRYGVMHRNSYMDSPNLLNQT
                     FATRKNPNLFFAGQMTGVEGYVESAASGLVAGINAVRRFNGESEVVFPQTTAIGALPH
                     YITHTDSKHFQPMNVNFGIIKELEGPRIRDKKERYEAIATRALKDLEKFLNY"
     gene            complement(10484..11182)
                     /locus_tag="VBC28_001399"
     CDS             complement(10484..11182)
                     /locus_tag="VBC28_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000936162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEO6815379.1"
                     /translation="MLPAYIKIHDAIKKEIDKGTWKIGQRLPSERDLADDYSVSRMTL
                     RQSITLLVEEGILERRVGSGTYVASHRVQEKMRGTTSFTEIVNSQGRKPSSKLISFQR
                     KLANETEIQKLNLSQSDYVVRMERVRYADKVPLVYEVASIPENLIKGFEQSEVTEHFF
                     KTLTENGYEIGKSQQTIYARNASERVASHLEVNAGHAILALTQVSYFTDGKPFEYVHG
                     QYVGDRFEFYLENN"
     gene            11395..12957
                     /gene="guaA"
                     /locus_tag="VBC28_001400"
     CDS             11395..12957
                     /gene="guaA"
                     /locus_tag="VBC28_001400"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEO6815380.1"
                     /translation="MTDISILNDIQKIIVLDYGSQYNQLIARRIREFGVFSELKSHKI
                     TADEIRDINPIGIVLSGGPNSVYADGAFGIDEEIFELGIPILGICYGMQLITHKLGGK
                     VLPAGEAGHREYGQSALRLRSESALFAGTPQEQLVLMSHGDAVTEIPEGFHLVGDSVD
                     CPFAAMENTEKQFYGIQFHPEVRHSVYGNDILKNFAVNICGARGDWSMDNFIDMEIAK
                     IRETVGDRKVLLGLSGGVDSSVVGVLLQRAIGDQLTCIFVDHGLLRKNEGDQVMDMLG
                     GKFGLNIIRVDASKRFLDLLSGVEDPERKRKIIGNEFVYVFDDEASKLKGVDFLAQGT
                     LYTDIIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRALGTALGMPDEV
                     VWRQPFPGPGLAIRVMGEITEEKLETVRESDAILREEIAKAGLDRDVWQYFTVNTGVR
                     SVGVMGDGRTYDYTIAIRAITSIDGMTADFAQLPWDVLKKISTRIVNEVDHVNRIVYD
                     ITSKPPATVEWE"
BASE COUNT         4188 a         2339 c         2077 g         4399 t
ORIGIN      
        1 atagatataa tggagatttt attatgaaac gtgaattact actcgaaaaa atcgatgaac
       61 taaaagaaat tatgccttgg tatgttctag aatattatca atcaaagctt tctgttcctt
      121 atagtttcac aacactctac gaatatttaa aagaatatag acgttttctt gaatggttat
      181 tggattctgg tgttgctaat tgtcatcaca ttgcagaaat tgagctttca gttttagaaa
      241 accttactaa aaaagatatg gaagctttta tactatattt acgagaacgc cctttattaa
      301 atgctaacac aaggcaaaat ggggtgtctc aaaccaccat taacagaact ctatctgcct
      361 tatctagtct tttcaaatac cttactgaag aggttgagaa tgctgacggg gagccttact
      421 tttatcgcaa tgtcatgaaa aaagtctcaa ccaaaaagaa aaaggaaact ttagcttctc
      481 gggcagaaaa tattaaacaa aagttatttt tgggcaatga aacgatagaa ttcttagagt
      541 atatagattg tgaatatcaa aacaaacttt ccaaacgtgc tttagctttt ttcaacaaaa
      601 ataaagagag agatttagct attattggtt tgctactagc ttcaggagtg cgtctatctg
      661 aggcagtaaa cttagattta aaggatatta accttaatgt aatggttatt gatgtcacac
      721 gaaaaggggg gaaaagggac tctgtcaatg tcgctagctt tgcgaagccc taccttgcta
      781 attatttaga catccgtaaa aatagataca aagcagaaaa tcaggacatt gctctatttt
      841 tatcagaata ccgtggggtg ccaaatcgta ttgatgcttc aagtgtagag aaaatggttg
      901 ctaagtattc ccaggatttt aaggttcgtg taacacctca taaactaagg catactcttg
      961 ctactaggct atatgatgct acaaaatctc aagtattagt tagccaccaa ctaggtcatg
     1021 cttccactca agttactgac ctatataccc atattgttaa cgatgaacaa aagaacgctt
     1081 tagataaact atagtaacat aaataaagtt atgtaaatat tttattttac ataactttat
     1141 ttatgtatag aaacagcagt cccataatta attttgctat ccaccgaaat ggacaaattc
     1201 agactagata agacttcttt tgtcatatat aaccccatgc ctgttgcctt ttggtgctca
     1261 tgaccgttgt aacccgtaaa gccatcttca aaaagtctag ggatatcttc ttcgagtatg
     1321 ccgataccgt agtcttgtat tctaatactc tcttctccta tgcttattat tatctttgac
     1381 tcaggattag aatattttat ggcattatct agcacctgtg aaagagcaaa agttaaccac
     1441 tttttgtctg ttttccagat attatcgcct tcaattatga tagataagct tttggataga
     1501 caaataacct tatacgattt tataatttct actactactt ctctaaggct aacagcttca
     1561 aaacgaaaat cgtcacgata ttgtctaaat ttcaaaaatg ctaacaacgt ttcaagataa
     1621 tgttgcaatt tcaataattg ttgttcaact tccttaggat cgagatgatt tgtctgggcc
     1681 attaatgaaa tagctgccaa tggaactttc atttggtgcg accacatctt tatcataagt
     1741 gcaacatgat tggtttgttc aattgtttca gaaacttttt gcgcttctct agcttctaat
     1801 tgtgtaataa tattaaaata agcgtaatca ctcggttgaa gttcgaaaga ggggtcctga
     1861 ctactattaa aatatttgag atgtaacatt tttttcctgt aacgactgta ttgccaaata
     1921 ctaattccta gtaaaacaat aacatttaaa cctaaggaat taaacaaata gggcattggt
     1981 aaatgatata gatagaaact aataaaaaat aggacaaaca tcatgatata taaaatatac
     2041 caaatcaagt gttctcttaa aaactggcgt atcattgtag taaataccca actcctctca
     2101 ctgtatgaat gtaatcaaaa cctattggga caattttttt acgtaagcgt gtcatattaa
     2161 cattaagtgt attttgatca ataaaactat cattttccca aagtttctct aatagagact
     2221 cttttgagac tacttgttta ggatgcatga gcaagataga taggatttta ttttctgttg
     2281 gcgataaaat aacctcttta tcttggctag acaataaccc ttctcttgtc aacgtaaatc
     2341 ccccaaaagt taattcctgt tggataaatt gttgacttct ccttaaaata gcagttagct
     2401 tagcatctaa tacagctaga gagaatggtt ttgaaataaa gtcatcaccc cccatattta
     2461 atgccataac catatccatt tcatcattac tagatgaaat gaaaataata ggaattgtta
     2521 aaaacttacg caattctgca gtccagtaaa aaccattaaa atagggtaac gtaatatcca
     2581 ttagtatcaa atcgggttga aatgcgataa tttcttgttt cacatcacga aaattgctga
     2641 cactagagac atgatagcta gctgataaat gatcttttaa aagtgacaca atcgtcatat
     2701 catcttctac aatataaatt tttccttgct cttgtgacat atcccctcct aaaatagtac
     2761 gaaatctctt tctttcattt agtataacaa attttttagt ctatcggttt ctattaatgc
     2821 aaaaggattg gtaaaataac caatcctttt tagcgttcaa taatatgata ataagttcga
     2881 ctagttatac ggtaaatgat aaagtaaatt atagaaatcg caagtactgt caaaccactt
     2941 actacatata cgattgttga gttaagcacc ccaaatacca aaagcatctg ttttagcatt
     3001 ggaatagcaa ctccaaagtg aataactgct accactaaag gttggaagaa gaaaatcata
     3061 atttgagaat taatagtccg tttaactaat tttttgctca tacctacttc ttgtaagata
     3121 cgataagatc tcttatcttc atgaccttct gagtattgct tatagtaaac aattagagct
     3181 atacctagca aaaagcttat acctaacaga aatccagtaa ataataaccc accaaacatt
     3241 tgataacctt cttttagaaa actctgttta gtgctaatat ttccaacata cttaccatct
     3301 acctctatta tgtcgttttt agaaattgaa tttatctctt gactattcaa atccaaatat
     3361 gccttaaagg tttttggaaa agtattgatg ttcttagtat aaggtaaata tgcttttcta
     3421 atattgtcaa tttgtttgtt gtttgcaaat ataatcaaac ccgggttgta agtatttgta
     3481 gtctctggga cataagcctc ttttaaattt tttacaacat caaatttatt tcccaataga
     3541 tttatttttt tcaaacggct atcacctttt tgaacaaagt aagccacttg gttatctttt
     3601 aattttggta attggtgacc taaacgacgg aaatcattct gagtaattag gtacataaaa
     3661 cccgtttttg tgatatcaac atgtttgacg tttttagatg tgatattaag ttcacttgat
     3721 tgactaactg gcatcgaaat cattgtctga ttataagtga tagcttcctt agatgactta
     3781 cctagtttct tcaaaatctt ttcttcaaat atatttttcg cgtcaccttt tgaattatct
     3841 attgataaac ttactgattt tggaaatagt ccggtaacaa cattttgtgt atttgaataa
     3901 agtgagactg ttgtagcaat agtaactaga gccataacag ctaataaagt gatacttgct
     3961 aaccctactg cattttgctt cattcgaaaa atcatttgcg aagttgatac aaaatgctca
     4021 gatttataat aataatgctt gttttgacgc aaccttttta agtaccatgt cataaaacta
     4081 atataaaaaa gataagtacc agcaataact aaaagtactg caaagaagaa acgatagata
     4141 acagctaatg caggtgcttt acctgaagta agagccattg tataagcgat ggcgatagct
     4201 actaacgcta aaattgcaag tataagatta ccttttggtt ctttttctcc ctgttgtttc
     4261 tttctaaaaa gacttaatgg ggatgataaa tgaacatgtc gaataactgg aacttctaaa
     4321 gtcagaaaaa tacctgtaaa tataacgata caaataataa atggccataa acttaaacta
     4381 agatttagtg catgatagtt aataatgttg acaaaaatta aataaataaa tttagcaaaa
     4441 aaagcactaa acagacttcc tatagaaata agaaatatat aaataataaa cagctctaga
     4501 ctagctacac gcgcaacttg acgtttattc atccccaaaa tgttatacag tccaaattca
     4561 ctactacgct gtttcatcaa gatattgtag ctataatgtt ccatgactac cgcaaagatt
     4621 gacaaaacaa tcactccaag aaaaagaacc gttgccgctg tccccatatc tcttccaaca
     4681 gcactcatta agattagtag cgtagaacaa gtcaatgaat aaagtaataa actggctaag
     4741 aggaatggta tgtactggtc tatagaatgt tttaaattat gccaggctat tttgagataa
     4801 aacatattat tcacccccta atatggcaga cattgttaaa gaaatatctt tattgaattc
     4861 gctattattc ttattaccac gataaagttg gtgaaaaata cgtccatctt tgataaataa
     4921 aacgcgtttg gcatgactag cagcatttgc tgaatgggtt accatcaaaa tagtttgtcc
     4981 atccaagtta atagtttcaa ataaattcag caagtcttct gaattacggt aatctaacgc
     5041 tgctgttggt tcatctgcta ataaaatttg agggttggtt atcaaactac gagcaatcgc
     5101 tacacgttgc ttttgcccac cagagagctc gaaaggtctc ttatctaata agtcatcaat
     5161 tctcagatga gaagataatt ctgacaaacg atgatccatt tctttgtaac gtttacgatc
     5221 aagaactaaa ggaagataga tattatctct tacagagaga gtatccaaaa ggttgaagtc
     5281 ctgaaagacg aaaccaagat ttttcaaacg aaaactcgct aattttgcct ctttaatttt
     5341 cgtaatatct tccccattta aaatcacttg tccatttgtc ggtttttcta aagtagctaa
     5401 aatatttagc agagttgttt ttcccgaacc agactctccc ataatagcaa tgaattcccc
     5461 ttgttcaact ttaaaatcga cgtcttgtag agcccttgtc tcttcttttg aaaaacgtgt
     5521 acgaaaaact ttttctaagt gattgatttc taataacata taattctcct ttatttatta
     5581 taccctaatt ttgattgatt ctactcttta accaagactg tgactcttgt aaaggaaggc
     5641 tggttacttg atctggtaag ataggggtgt tctcgtattt atatccttgt cgatcactaa
     5701 ctattcctgt agttaaagct aatctagcac cgtcgtaaag catgaaagtt tcattaacag
     5761 tcgtatatcc tgctgtagct tgaccaaagc ttttaacatt aggtaatcct ttaaatgata
     5821 aaaaagtcat ttctgccgaa ctagcagttt tatgattagt tataatagcg attggaacat
     5881 gcttagtatt gattgttttc ctagatattt ttaatgcttc tagcggtata tttttcatcg
     5941 tgattgtttt cttatttcca tacttatcag tataatgaaa tagggtatca tttggtaaaa
     6001 tagaggctac ccccccaatc atagggatca tattgccgcc agtattattc gataagtcta
     6061 aaatgagtcc cttataactt ttttccatca atatattttg taatttacca ctataatggc
     6121 taatagatcg tttatctaac ttacttatag aaggaatagt aattaagaga atgtcttcgt
     6181 ttaatcttcg atattctgga tatcgactat ctagggtatc tcttcgtaca gtctctttgg
     6241 ataatatttg actatgttta ccgcctccat accgtactgc ttcctgtaca atgttgcgac
     6301 tctcttggta agattttgac gttttcaact ttttttctga tcgttgtctt ataatttgcc
     6361 attgacgacc ttgcgaatac aaaccacgct gttcaacacg atctaaaata acacgtccat
     6421 aacgttcgct actaggaggt aatagataaa tatttaatgc tgaaccataa taatgaacta
     6481 ccactcctaa aattcctaaa actataagca ttggaactac aaaaagtaaa actatttttc
     6541 gccgcatctc ttgctccccc tttctcttta agtctattat atggcaaaat ggtttactat
     6601 aaccttacat cttaagacct atttcttaca aatttgaaag ttttaaaagc tttataaact
     6661 ataattttga tataatatct tacataacaa atggaaagta cggtgaaaac atatggccaa
     6721 gacaagtaaa aaattgacaa aatctttagg gcctattggt aaattaattt caataatccc
     6781 tgacacaact gaacttattg gaaaagcaat tgataattct cgtccaatta ttgaaaaaga
     6841 acttgaccgt cgtcatgaaa aaaaaacaga cttatactca gcttgataat gtagtacacc
     6901 ttgatatcaa tgatgcgcaa gtttacttag agcaacgcca ttttaatgta tcaaggattg
     6961 ttgctgaacc aaattcaaag tgggcaaccc tacatcctaa tcaagttgtg aatatgtcgc
     7021 ctaaagctgg tagggtcaga atcggttcat tagtgaagtt gtactatatt acagaggata
     7081 tccttgaaga aagtaagaca ctacttttat ctaaatccga aaaaagtaaa gcacgacaac
     7141 aacggttaac agaagtattg agtaccccta gtcaattact caaaaaaaat cgaaataaat
     7201 aagcatgatc gaattgacca tgcttattta tttttaccaa acaggttgat ctaaaccttg
     7261 agatgttttt tcgataactt ttttaaccgc atctgtatgg taagctttca caacttcttt
     7321 aatggcttta gcttttgatt tgtcttgcca acctttttga gcaactaaaa ggttgtacca
     7381 ttgttttgca tttttacttt ttggttcaat aaaaatagct gatttaggat ttattcctgc
     7441 ttctgtcaca aaatcattat taataactgc tgcatctgtg gaatccaaag aacgtgcggt
     7501 ctgtgctgca tctacttctt ttaaatctaa agatttagga ttggaaacaa catctgacat
     7561 tgttgctaat gcatcacctg atactttcaa ttttagcaag cctgctgatt gtaacaagta
     7621 gagagagcga ctttcgttaa ctgcatcgtt tgggatagta atagttgctt tatttggaat
     7681 ttcagaaact gtttggtatt tacctttacc gttcttagta ccagagtata atctaaagga
     7741 agtaaagtat gtctcagcaa cggaaacaag attggtctta tttgctttat tccaattgtt
     7801 taagtaatta taatgctgga aagcattaat atcaatctca tcactttcta aagctttatt
     7861 aggttgtgta taatctgtga attccgtaaa ttttagctta acgccttttt tcttaactaa
     7921 ttcttcgatt ttatcccaac gagctttttc agttttactc tttaccattg ttccaactct
     7981 aacgactttg gcgtcttcat ttttagactg accacaggct cctaaaataa gtgctgaaat
     8041 tactactgtt gtcgttaacc ctaatagctt tttgattttc ataatttatt tctcctttga
     8101 tatggttgta tgattaatca actgatacta gtgtaacaaa aagctaatgg attaaaaaat
     8161 acttattatc aatcaagaga tatagctttt aactatatca tttcttttat aattttactt
     8221 tcgagggctc cgtaaagcgg tccaataata ggcgacttcc tcataacctt ggtgctcata
     8281 catttttctg ttttcttctt ctgaaattaa gataactttc ttaaaccccc tacttgcatc
     8341 aaattgtagg cgcgtaccaa ttccttgtcc tctgtaggac tcttggacat aaaaatcatc
     8401 tatttccacg tattcaccat agtcccaagc tgttacatct ccaataattt gattatcttt
     8461 aatagctagg taaactttag cattattatt taaaagatta acacgattat cattatacat
     8521 ttgcttagcg taaatttctc cgtaaactat actttgttta tatttgaact ttaggtattt
     8581 ttctaggtaa ttttcattaa gtttagtaaa tgtgatgtca tcaaattctt tttcaaaaag
     8641 tttaagatga tttaatgaat ttgtaaatat gatatgtttc tctgattcaa agccttgttg
     8701 tgataaagtt ctctgtaatt cactgctcaa ttcagcattt tcctcaaaaa atacaaaagc
     8761 aaaatcagac tgataatctt tttgttcatc tgctaaatag atcatatcct gtaaaaactc
     8821 acttactgta ggcattattt tgtagctaat atagtttgaa aaataacgct ctgaaatgat
     8881 tatattctta aaatgtttat accgattagt ttccaaataa gctttttcgg atgactcaag
     8941 taattcttta tagtgcatat ttactccatt catacatttt taaaaatcaa tatagaaaat
     9001 ttgaccttgc ttgttggcaa agtcaaatga atatatcaat agttgagaaa tttttctaaa
     9061 tctttcaaag ctcgtgtagc aatagcttca taacgctctt tcttatcacg aattcgtgga
     9121 ccttctaatt ctttgatgat accaaagtta acattcattg gttggaaatg tttgctgtct
     9181 gtatgtgtaa tataatgtgg cagtgcacca atagcagtag tttgtgggaa aactacttca
     9241 ctctccccat tgaagcgacg gacagcattg attcccgcta caagaccact tgctgccgat
     9301 tctacataac cttcaacacc ggtcatttgt ccagcaaaga aaagatttgg gttcttacgt
     9361 gtagcaaagg tttggtttaa aagatttggt gaatccatat aagaattgcg atgcataaca
     9421 ccataccgga caaattcagc attttcaaga ccaggaatca tttgaaatac acgtttttgt
     9481 tctccccact taagatgagt ttggaaacca actatgttat aaagagagcc tgcagcatta
     9541 tcttgacgta attgaacaac agcatatggt gttttaaatt caccatctcg cggtcctttg
     9601 tagtcctcag gatactccaa accaactggt ttcattggac cataaagcat cgttttaata
     9661 ccacgcttag ccatcacttc gataggcata caaccttcga agtatttttc tttctcaaaa
     9721 gaatttagtg gtgcttcctc agctgtagtt aaagcttcat gaaaagccat aaattcttct
     9781 ttggtcattg gacaatttag ataagctgct tcacctttgt catacctaga cttaaggtaa
     9841 actttattta tgtcaatcgt atttttatcg acaattggag cggcggcatc gtaaaaataa
     9901 aaaccatcac caccattaag ttcatggatt ttagctgcca gactatcact tgttaatggt
     9961 cctgtagcga taactgtaat agcatctcca gggatatctg tgatttcatc acgaattact
    10021 tcgattaatg gatgtttatg gatttcctca gtaacagctt cagaataacc ttctctatca
    10081 acggccatgg ctccacctgc tggaactcga tgtgcctccc catttctcat aatgatagaa
    10141 tccaaacgac gcatttcttc ttttaaaaga ccaactgcat ttgttagaga atcaccgcgg
    10201 aatgaattgg aacatactaa ttctgcaaaa ttatctgttt tatgttgagg ggttgattta
    10261 accccacgca tttcataaag cttaactggt attccacgtt tagcaatttg ataagctgct
    10321 tcagaaccag ccaaaccagc gccgataaca ttaatgtaag attgagacat gaactaatac
    10381 ctcttgaaag tagtcaacta cttcctcaaa tctttctata taatttattt ttccattata
    10441 acatacatag ctacttcttc aaaaaacgtt attcattaaa ctactagtta ttctctaaat
    10501 aaaactcaaa acgatctcca acatattgcc catgaacata ttcaaaaggc ttaccatcag
    10561 taaagtatga aacttgtgtc aatgccaaaa tagcatggcc tgcgttaact tctaaatgcg
    10621 aagcaacacg ttcactagca tttctagcat atatagtttg ttggcttttc ccaatttcgt
    10681 agccattctc tgttaaggtt ttaaagaaat gttctgtaac ctctgattgt tcaaaacctt
    10741 taattaaatt ctctggtata gaggctacct cataaaccaa cggtacctta tcagcataac
    10801 gtaccctttc catacgaacc acataatctg actgtgataa atttaatttt tgaatttcgg
    10861 tttcatttgc tagttttctt tgaaatgaga ttagtttact ggatggctta cgcccttgag
    10921 aattgacaat ttctgtaaaa ctagttgttc cccgcatttt ttcttgaaca cgatgactag
    10981 caacataggt gccactacca actcgtctct ccaaaatacc ttcttcaact aacaatgtaa
    11041 ttgattggcg aagtgtcata cgacttacac tataatcatc cgctaaatct ctttcgctag
    11101 gtaaccgctg cccaatcttc caagtccctt tatcgatttc ctttttaata gcgtcgtgaa
    11161 ttttaatata tgctggtaac aatattctct cctcctagaa tctacacttc attttaccat
    11221 acttctatat atttctaact ctaaaatcgt gaaataattc agaaaaatca taagcaatat
    11281 ttcttgataa gaaagcgttt aatcaattat tttaatgatg ttaatttata taaaatatgc
    11341 taaaatggta taggtacagc tgtttttaaa attagctaca aagaaaggga aacaatgact
    11401 gatatttcta tattgaatga tatccaaaaa attatcgttc tagattacgg tagtcaatat
    11461 aaccagctta tcgcacgtcg tatccgtgaa tttggtgtat tttctgagtt aaaatcacat
    11521 aaaattacgg ctgatgaaat tcgcgatatt aatcctattg gtatcgtact ttcaggtggt
    11581 ccaaattccg tatacgctga tggcgctttt ggaatcgatg aagaaatttt tgaattagga
    11641 attccaatct taggaatttg ttatggcatg caactgatta cccataaact cggtggtaag
    11701 gttcttcctg cgggagaagc tggtcatcgt gagtatggtc aatcagcact tcgcctacgc
    11761 tcagaatcag cactctttgc tggcactcct caagaacaac ttgttttgat gagccatggt
    11821 gatgctgtca ctgaaattcc tgagggtttc catctcgttg gtgactctgt tgactgtcca
    11881 tttgctgcaa tggaaaatac tgaaaaacaa ttttatggta tccaattcca ccccgaagtt
    11941 cgtcactcag tatatggcaa tgatatcctt aaaaattttg ctgttaatat ctgtggcgca
    12001 cgtggtgatt ggtcaatgga taacttcatt gatatggaaa ttgcaaaaat acgtgaaact
    12061 gtaggcgatc gtaaagtact tcttggacta tctggcggtg ttgattcatc tgttgtcgga
    12121 gtactcctac aacgtgctat cggtgatcaa ttaacatgta tttttgtaga tcatggttta
    12181 ttacgtaaaa atgaaggtga ccaagttatg gatatgcttg gtggtaaatt tggacttaat
    12241 attatccgtg ttgatgcttc aaaacgcttc ctagatcttc tttctggtgt tgaagatccc
    12301 gagagaaaac gaaaaatcat cggaaatgag ttcgtgtacg tttttgatga tgaggcaagt
    12361 aaacttaaag gcgttgattt ccttgctcaa ggtaccctct acacagatat tattgaatct
    12421 gggacagaaa cagcccaaac aatcaagtca caccataatg ttggtggatt gccagaagat
    12481 atgcaatttg aattgattga gccacttaac acattattca aagatgaggt tcgcgctctt
    12541 ggtactgcac ttggaatgcc tgatgaagtt gtatggcgtc aaccattccc tgggcctggt
    12601 cttgctattc gcgttatggg tgaaattacc gaagaaaaac ttgaaacagt ccgcgaatca
    12661 gatgctattc ttcgtgaaga aatcgctaaa gctggtctag atcgtgacgt atggcagtat
    12721 ttcacagtta atactggcgt acgttctgtc ggtgttatgg gagatggtcg tacttatgac
    12781 tatacaattg ctatccgtgc aattacttca attgatggca tgacagctga ctttgcgcaa
    12841 cttccttggg atgtacttaa gaaaatttcg acacgtattg ttaacgaagt tgatcacgtt
    12901 aaccgtattg tttatgatat tacaagtaaa ccacctgcaa ctgttgagtg ggaataaaaa
    12961 cttagctact ttaagtagct aagttttttt actcttattt ctt
//
LOCUS       DATRMA010000050        12997 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.050, whole genome shotgun sequence.
ACCESSION   DATRMA010000050 DATRMA010000000
VERSION     DATRMA010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12997
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.050"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            complement(124..1476)
                     /locus_tag="VBC28_001401"
     CDS             complement(124..1476)
                     /locus_tag="VBC28_001401"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEO6815381.1"
                     /translation="MKVVKFGGSSLASSQQLYKVLNIIKSDYTRRFVVVSAPGKRYEE
                     DLKMTDALIQYYQNYINGKDIVKDQTWIINRYQEIISDLSLGSTIAEEITRSIEQLAS
                     LPIENNQFLYDCFLAAGEDNNAKLVATFFNQNDIPARYVHPNEAGIIVTKEPCNARII
                     PGSYDKIENLCLYNEVLVIPGFFGVTEDNQICTFSRGGSDITGSLIAAGIKADLYENF
                     TDVDGIFAAHPGVVKNPHAIPELTYKEMRELAYAGFSVLHDEALLPAYRGRIPLVIKN
                     TNNPQQPGTKIVLKHTRSNIAVTGIASDSRFASINVSKYLMNREVGFGRKVLQILEDL
                     NISFEHMPTGIDDLSIVLREKELTPIKEQEILNYLTRKLEVDYVDIQHNLSTIVIVGE
                     NMKSQIGVTATATQALSREKINITMISQGSSEVSIMFVINSKDEKRAIKALYETFFQK
                     "
     gene            1570..2220
                     /locus_tag="VBC28_001402"
     CDS             1570..2220
                     /locus_tag="VBC28_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001900118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEO6815382.1"
                     /translation="MCKYKAIIFDMDGVLFDTELFYYKRRERFLKQHGITIDHLPMNF
                     FIGGNMKQVWKSVLGDQYDTWDIDKLQQDYSRYKEDNPLPYKDLIFQDCKRVIEKLHH
                     KGYLLGLASSSTRHDIMLALESFNLDTYFKVILSGEEFSESKPNPAIYNRAAELLDIP
                     KQQILIVEDSEKGITAGIAAGIDVWAIEDKYFGMNQSQANVLVSDLSQFFVKLDNY"
     gene            2357..3148
                     /locus_tag="VBC28_001403"
     CDS             2357..3148
                     /locus_tag="VBC28_001403"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000667362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEO6815383.1"
                     /translation="MKFENIIYGIDGNVATIMLNRPDISNGFNIPMCQEIIDAIRLVS
                     ENKDVMFLVIEAQGPIFSIGGDLKVMKAAVESDDISSLTKIAELVNQISYDLLQLEKP
                     VVMCVDGAVAGAAANIALAADFVIASKKSKFIQAFVGVGLAPDAGGLLLLSKSIGITR
                     AVQLALTGESLSAEKAEALGIVYKLCESDKIGKIKDQLLKRLSRHSINSYQAIKSLAW
                     EAAFKDWEQYKKLELQLQESLAFKQDFKEGVRAHADRRRPNFLGK"
     gene            3244..3678
                     /locus_tag="VBC28_001404"
     CDS             3244..3678
                     /locus_tag="VBC28_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEO6815384.1"
                     /translation="MEYDQINSYLVDIFNRIMIIEEMSLKTSQFSDVSLKEMHTIEII
                     GKHSEVTPSDVARELMLTLGTVTTSLNKLEKKGYIERKRSSIDRRVVHLSLTKRGRLL
                     DRLHSKFHKSMVSHIIEDLGEEDIKMLTSALGNLHKFLEDLV"
     gene            3678..4649
                     /locus_tag="VBC28_001405"
     CDS             3678..4649
                     /locus_tag="VBC28_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HEO6815385.1"
                     /translation="MVFAKISQLAHYAPSQIIKNEDLSLIMDTSDDWISSRTGIKQRH
                     ISKNETTADLANKVAEQLIEKSGYSASQIDFIIVATMTPDSMMPSTAARVQAHIGASN
                     AFAFDLSAACSGFVFALSTAEKLISSGSYQKGLVIGAETVSKVLDWTDRGTAVLFGDG
                     AGGVLLEASKEKHFLAESLNTDGSRQGLQSSQVGLNSPFSDEVLDDKFLKMDGRAIFD
                     FAIKEVSKSINHLIETSYLEKEDIDYLFLHQANRRILDKMSRKIDIARDKFPENMMDY
                     GNTSAASIPILLSESYENGLLKLDGNQTILLSGFGGGLTWGSLIVKI"
     gene            4707..4931
                     /locus_tag="VBC28_001406"
     CDS             4707..4931
                     /locus_tag="VBC28_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEO6815386.1"
                     /translation="MAVFEKVQEIIVEELGKDAEEVTLNTTFDDLDADSLDVFQVISE
                     IEDAFDIQIETEEGLNTVGDLVAYVEEKVK"
     gene            5086..6045
                     /gene="fabK"
                     /locus_tag="VBC28_001407"
     CDS             5086..6045
                     /gene="fabK"
                     /locus_tag="VBC28_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HEO6815387.1"
                     /translation="MKTRITELLNIKYPIFQGGMAWVADGDLAGAVSKAGGLGIIGGG
                     NAPKEVVKANIDKIKSMTDKPFGVNIMLLSPFVDDIVDLVIEEGVKVVTTGAGNPGKY
                     MERFHEAGITVIPVVPSVALAKRMEKLGADAIITEGMEAGGHIGKLTTMTLVRQVVDA
                     VTIPVIAAGGIADGRGAAAGFMLGADAVQVGTRFVVAKESNAHPNYKAKILKAKDIDT
                     AVSAQVVGHPVRALKNKLVTTYSQAEKDYLAGRISINEIEELGAGALRNAVVDGDVIN
                     GSVMAGQIAGLIKSEETCQEILEDIYSGARQVILSEASRWSDL"
     gene            6065..6991
                     /gene="fabD"
                     /locus_tag="VBC28_001408"
     CDS             6065..6991
                     /gene="fabD"
                     /locus_tag="VBC28_001408"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEO6815388.1"
                     /translation="MNKVSFLFAGQGAQKLGMARDLYETFPIVKETFDKASHVLGYDL
                     RELIDKDLDKLNQTKYTQPAILTTSTAIYRLILKEIELRPDMVAGLSLGEYSALVASG
                     AIRFEDAVVLVARRGQLMEAAAPAGSGKMVAVLNADRQIIEDACKKASQFGIVSPANY
                     NTPKQIVIGGESIAVNAAVEELKQQGVKRLIPLNVSGPFHTALLKPASQKLSDVLDKV
                     HFSVSEIPVIGNTEAQIMKKDDIKSLLARQVMEPVRFDESIETMKKMGMTQVVEIGPG
                     KVLSGFLKKIDSSLSVHSVEDKIGFNNLKELN"
     gene            7000..7734
                     /gene="fabG"
                     /locus_tag="VBC28_001409"
     CDS             7000..7734
                     /gene="fabG"
                     /locus_tag="VBC28_001409"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEO6815389.1"
                     /translation="MQLKDKNIFITGSSRGIGLAIAHQFAQLGANIVLNGRSEISEDL
                     IAEFADYGVKVIAISGDVSSFEDANRMIKEAIASLGSVDVLVNNAGITNDKLMLKMTV
                     EDFESVLKINLTGAFNMTQSVLKPMTKARQGAIINISSVVGLTGNVGQANYAASKAGL
                     IGFTKSVAREVAARGIRVNAIAPGFIESDMTDVIPEKMQEAILAQIPMKRIGKGKEVA
                     QVASFLAEQEYLTGQVIAIDGGMTMQ"
     gene            7750..8982
                     /gene="fabF"
                     /locus_tag="VBC28_001410"
     CDS             7750..8982
                     /gene="fabF"
                     /locus_tag="VBC28_001410"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012679081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEO6815390.1"
                     /translation="MTLQRVVVTGYGVTSPIGNTPEEFWNSLKEGNVGIGPITKFDSS
                     DFMVKNAAEIHDFPFDKYFVKKDLNRFDMYSLYALYASSEAIQHANLNLDEIDADRFG
                     VIVASGIGGIQEIEEQVIRLHEKGPKRVKPMTLPKALPNMAAGNVAMRLGAHGVCKSI
                     NTACASSNDAIGDAFRNIKFGIQDIMVVGGAEAAITKFAIAGFQSLTALSTTEDPSRA
                     SIPFDKDRNGFIMGEGSGMLVLESLEHAEKRGATILAEVVGYGNTCDAYHMTSPHPEG
                     LGATKAIQLALVEANIKPEEVNYVNAHGTSTPANEKGESQAIVAALGTDVPVSSTKSF
                     TGHLLGAAGAVEAIATIEAIRHSYVPMTAGTTELSEDITANVIFGQGQDADIRYAISN
                     TFGFGGHNAVLAFKRWED"
     gene            8948..9484
                     /locus_tag="VBC28_001411"
     CDS             8948..9484
                     /locus_tag="VBC28_001411"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001878034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEO6815391.1"
                     /translation="MQFWPSNAGRIKLDIQEIKDLMTQFDESSLREFSFKTSDGELSF
                     SKNEGKAPLVPTMSPMSHQPEATPTIATPVSNEAGEQTKQATEVVSEIPESTVTVAEG
                     DVVESPLVGVAYLASGPDKPNFVSVGDSVKKGQTLMIIEAMKVMNEVPAPHDGVVTEI
                     LVANEEVIEFGKGLVRIK"
     gene            9481..9903
                     /gene="fabZ"
                     /locus_tag="VBC28_001412"
     CDS             9481..9903
                     /gene="fabZ"
                     /locus_tag="VBC28_001412"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEO6815392.1"
                     /translation="MIDIKEIREALPHRYPMLLVDRVLEVSEDEIVAIKNVSINEPFF
                     NGHFPEYPVMPGVLIMEALAQTAGVLELSKEENKGKLVFYAGMDKVKFKKQVVPGDQL
                     VMTAKFVKRRGTIAVVEAIAEVDGKLAASGTLTFAIGN"
     gene            9941..11311
                     /gene="accC"
                     /locus_tag="VBC28_001413"
     CDS             9941..11311
                     /gene="accC"
                     /locus_tag="VBC28_001413"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEO6815393.1"
                     /translation="MFKKILIANRGEIAVRIIRAAREMGISTVAIYSEADKESLHTIL
                     ADEAICVGPAKSAESYLNVNAILSAAIVTGAEAVHPGFGFLSENSKFATMCEEMNLKF
                     IGPSGEVMDKMGDKINARTEMIKADVPVIPGSDGQVTSVEEAVSIAEEIGYPLMLKAS
                     AGGGGKGIRKVKSADELKPAFESASQEALAAFGNGAMYIEKVIYPARHIEVQILGDSF
                     GKIVHLGERDCSLQRNNQKVLEESPSVAIGNTLRQQIGEAAVRAAEAVSYENAGTIEF
                     LLDENSGQFYFMEMNTRVQVEHPVTEFVTGVDIVKEQIRIAAGIPLSVSQNDIKLTGH
                     AIECRINAENPQFNFAPCPGTINGLHLPAGGMGLRVDSAVYTGYTIPPYYDSMIAKVI
                     VHGENRFDALMKMQRALYELEIDGIVTNTEFQMDLISDKKVLAGDYDTSFLMEDFLPR
                     YYGNNQ"
     gene            11320..12195
                     /locus_tag="VBC28_001414"
     CDS             11320..12195
                     /locus_tag="VBC28_001414"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HEO6815394.1"
                     /translation="MALFSKKDKYIRISPNKALGSSDKRSLPEVPDELFAKCPSCKHM
                     IYQKDLGLAKICPACSYNFRISAQERLLLTVDEDSFEELFTGIETKDPLNFPNYREKL
                     AATRQKTNLDEAVVTGLAKIKGQTTALAIMDSHFIMASMGTVVGEKLTRLFELATEKK
                     LPIVIFTASGGARMQEGIMSLMQMAKVSAAVKRHSNQGLFYLTILTDPTTGGVTASFA
                     MEGDIILAEPQALVGFAGRRVIETTVREDLPEGFQKAEFLLEHGFVDAIINRTELRDC
                     IAQLIAFHGNEVGHE"
     gene            12188..12961
                     /locus_tag="VBC28_001415"
     CDS             12188..12961
                     /locus_tag="VBC28_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001058273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HEO6815395.1"
                     /translation="MNNDVTRILKDARDQGRLTALDYAELIFDNFMELHGDRQFADDK
                     SIIGGLGYLAGRPVTIVGIQKGKNLQDNLDRHFGQPHPEGYRKALRLMKQAEKFGRPV
                     ITFINTAGAYPGVGAEERGQGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALAVAD
                     KVWMLEHTVYSILSPEGFASILWKDGTRTTEAAQLMKMTAGELYHMEVVDKVIPEHGY
                     FSSEIVDMIKTSLISELEVLSQLSLEDLLEQRYQRFRKY"
BASE COUNT         4182 a         1939 c         2776 g         4100 t
ORIGIN      
        1 tgataccaag tggttacgat taaaagtgga aaaactatcc tatgttctaa ataaaatcgt
       61 cctaagtaag cttacttagg acgatttgtt atctatctaa tagtgtaagt agtgtaatag
      121 gtactatttt tggaagaatg tttcatatag tgctttaata gctctttttt catccttact
      181 gtttataacg aacataatgg agacttcgct tgaaccttgt gatatcatgg tgatattgat
      241 tttttctctt gataaggctt gtgtcgctgt tgcagtgact ccaatctgac ttttcatatt
      301 ttcacctaca attacgattg tagatagatt gtgttggatg tcaacgtaat ctacttctag
      361 tttacgagtt aggtaattta agatttcttg ttctttgatt ggtgtcaatt ctttttcacg
      421 taagacaatg gatagatcat ctatgccagt tggcatatgt tcaaaactaa tatttaaatc
      481 ctctaaaatt tgtagtactt ttcggccgaa acctacttct ctattcatta agtatttaga
      541 tacgtttatg ctagcaaaac ggctatcaga agcgatccca gttactgcta tgttactacg
      601 agtatgcttt aaaactattt ttgtaccagg ctgttgggga ttatttgtat ttttaataac
      661 aagagggatt ctgccacgat aggcaggaag taaagcttca tcatgtaaaa ccgaaaaacc
      721 cgcataggct aattcacgca tttctttata agtaagctca gggatagcgt gagggttctt
      781 aactacacct ggatgtgctg caaatatacc atcaacatct gtgaagttct cataaaggtc
      841 tgcttttatg cctgctgcaa ttagggatcc agtaatgtca gatccccctc ttgaaaaggt
      901 acaaatttgg ttatcttctg tgactccaaa aaatccaggg ataacaagaa cctcattgta
      961 tagacataag ttctcaatct tatcataact tcctggaata attcgtgcat tacatggttc
     1021 tttagttaca ataattcctg cttcgtttgg atgaacatat cttgcaggaa tgtcattttg
     1081 attaaaaaac gttgcaacta actttgcgtt gttatcttca ccggctgcta aaaaacaatc
     1141 atataaaaac tgattatttt ctatgggaag tgaagctagc tgctctatag agcgagtaat
     1201 ttcttctgca atagtgcttc ctaaagacaa atcactaata atttcttgat aacgattgat
     1261 aatccaagtt tggtctttaa caatatcttt accgttaata taattttgat aatattgtat
     1321 caaagcatct gtcattttta agtcttcttc gtagcgtttc cctggagctg aaacgacaac
     1381 aaaccgtcta gtgtagtctg atttgataat atttagtact ttgtataatt gttgggaaga
     1441 tgctaaagaa cttcctccaa atttgactac tttcataggt tacctcaaga atgaatttta
     1501 gataaattat aacaaaagtc agaaaatttg tcattacctc taaatttaaa agaatgatat
     1561 aatagttaca tgtgtaaata taaagcaata atatttgata tggatggtgt tctatttgac
     1621 actgaattat tttattataa aaggcgagag cgttttttaa aacaacatgg aattacaatt
     1681 gaccatttac caatgaattt ttttattgga ggaaatatga agcaggtttg gaaatctgtt
     1741 ttaggtgatc aatatgatac ctgggatatt gataagctcc aacaagatta tagtcgctat
     1801 aaagaggata atcccttgcc atacaaagat ttaatttttc aggattgtaa aagagtcatt
     1861 gaaaagcttc atcacaaagg ctatcttttg ggtctagctt caagttctac tagacatgat
     1921 atcatgttag cattagaaag ttttaatctg gatacatatt ttaaggtaat tctgtctggt
     1981 gaggaatttt cagaaagtaa acctaatcca gctatttata atagagcagc agaactttta
     2041 gatattccta aacagcaaat tcttattgta gaagacagtg agaaggggat tacagctggt
     2101 attgcagccg gtattgatgt gtgggctatt gaagataaat actttggaat gaatcagtcg
     2161 caggctaatg tattagtgag cgatctgagt caattttttg tgaaattaga taactattaa
     2221 aaacgggaag tttaattccg tttttttatt ttttatcttg ccatgaaagc taggtttatg
     2281 ataaaataca aagcatgata atttgataat caaattattt tttatttttc tttcactctt
     2341 aaagagagga gacattttga agttcgaaaa tataatatat ggtattgatg gcaacgtggc
     2401 cactattatg cttaatcgac cagatatttc aaatggtttt aacatcccaa tgtgtcaaga
     2461 aattattgat gcaataaggc ttgtgtctga aaataaagat gtcatgtttt tagtgattga
     2521 agcacaagga ccaatctttt caatcggagg agatttaaaa gtgatgaaag ctgctgttga
     2581 gagcgatgat attagctcat taactaaaat tgctgagtta gttaatcaga tatcgtatga
     2641 tttactacag ctagagaaac ctgttgttat gtgtgtagat ggggctgtag ctggtgccgc
     2701 agctaatatt gcattagcag ctgattttgt tatcgcaagc aagaagagta aatttatcca
     2761 ggcttttgtg ggtgtcggtt tagctccaga tgcaggaggt ttattgttac ttagtaaatc
     2821 aatcggtata actcgtgcag tgcaactagc tttaactgga gaaagcttat ctgctgaaaa
     2881 agctgaagct ttgggaattg tttataaact ttgtgaatca gataaaatag gtaaaattaa
     2941 ggatcaacta ttaaagcgtt taagtagaca ttctataaac tcatatcaag ccataaaatc
     3001 attagcttgg gaagctgctt ttaaagattg ggaacaatat aaaaagcttg aattacaact
     3061 gcaagaatca ttagccttta agcaagattt taaagaaggt gttcgggcac acgctgatag
     3121 aaggcgtcct aattttttag gcaaatagtt tgaaaatcaa attatttaag attaaagtct
     3181 tgcaaataag tttgaacttt gatataattt gattatcaaa gtttaatgtg aggtggtgat
     3241 atcttggaat acgatcagat taatagttat ttagttgaca tttttaatag aatcatgatt
     3301 attgaggaaa tgagtttaaa gactagccaa tttagcgatg tatctttaaa agaaatgcat
     3361 actattgaaa ttattgggaa acattctgag gtaactccta gcgatgtcgc tcgagaatta
     3421 atgttaactt tgggaactgt aacaaccagc cttaataagt tagaaaaaaa aggctacatt
     3481 gagcgtaaac ggtcaagtat tgatcgtcga gttgttcact tgagtcttac taagagaggt
     3541 aggttattag atcgattgca tagtaaattt cataaatcta tggtatctca tattattgaa
     3601 gacctaggtg aagaagacat aaaaatgttg acgagcgctt tagggaatct tcataaattt
     3661 ttggaggact tagtctagtg gtttttgcta aaattagtca acttgcacat tatgcacctt
     3721 ctcaaattat taaaaatgag gatttatcat taatcatgga taccagtgat gactggattt
     3781 cgtcacgtac tggtataaaa caacgtcata ttagtaaaaa tgaaacaaca gctgatttgg
     3841 caaataaggt cgcagaacaa cttattgaaa aatccggtta ttccgctagt caaattgact
     3901 ttattattgt tgcaacgatg acaccagact ctatgatgcc ttctactgct gcaagagtgc
     3961 aagctcatat tggagcgagt aatgcttttg cctttgactt gagtgcagca tgtagtggtt
     4021 ttgtttttgc cttgtctacc gccgaaaaat tgatatcatc aggtagctat caaaaaggtt
     4081 tagtaatagg agcagaaact gtttctaaag tattagactg gactgataga gggacagcag
     4141 ttttatttgg agatggagct ggaggtgtct tgctagaagc ttctaaggaa aaacattttc
     4201 ttgcagagtc tctcaataca gatggtagtc gtcagggact tcaatccagt caagtcgggt
     4261 taaattcacc tttctcagat gaagtacttg atgataaatt tttgaaaatg gatggtagag
     4321 caatctttga ttttgctatc aaagaagttt caaaaagtat caatcatctt atagaaacat
     4381 cttatttgga aaaagaagat attgattatc ttttcttaca ccaagcgaat cgaaggattt
     4441 tagataagat gtctcgaaaa attgatatag cacgtgataa atttccagag aatatgatgg
     4501 attatggtaa tactagtgca gctagtatcc ctattttact ttcagaatct tatgagaacg
     4561 gtttgttgaa actagatggt aaccaaacaa tcctcttatc gggtttcggt ggaggtttga
     4621 catggggaag tctaattgtt aaaatttagt tatatatagt gcatagcaca tttataaaag
     4681 attatttaaa atttaaggag ctaaaaatgg cagtatttga aaaagtacaa gaaattattg
     4741 ttgaagaact tggtaaagat gcagaagaag taacgttgaa cactactttt gacgatcttg
     4801 atgctgattc gcttgatgtt ttccaagtga tttcagaaat tgaagatgca tttgatatcc
     4861 aaattgagac agaagaagga ctaaatactg ttggtgattt agttgcttat gttgaagaaa
     4921 aagtaaaata attaagtcgt aagaaaaaaa gaagcaagtg ataataaaag atattcttac
     4981 caaatcactt attagagtga gtattcaatc aatcgttgat cggtactcac tcttcttctt
     5041 atcaatatag tttgattgtc aaattattaa ttttaggaaa gaaaaatgaa aacacgtata
     5101 acagaattac taaatataaa atatccaatt tttcaagggg gtatggcgtg ggtcgctgat
     5161 ggagatcttg caggagcagt ttctaaggca ggtggtctag gtattattgg tggaggaaat
     5221 gcccctaaag aggttgtcaa agcaaatatt gataaaataa aatctatgac tgataaaccc
     5281 tttggtgtga atattatgct tttatcacct tttgtagatg atattgttga tttggttatt
     5341 gaagaaggtg taaaagttgt aacgacgggt gcaggtaatc ctggtaagta catggagcgc
     5401 tttcatgaag caggtataac agtaatccct gttgttccta gtgttgcatt agcaaaacgt
     5461 atggagaaat taggggctga tgctatcatt acagaaggaa tggaagcggg tggtcacatc
     5521 ggaaaactga ccacgatgac attagtacga caggttgttg atgctgttac aattccagtc
     5581 attgccgcag gtggtatagc agatgggcgt ggggctgctg cgggtttcat gttaggagct
     5641 gatgctgttc aggtaggaac tcgttttgtt gttgctaaag aatctaatgc tcaccctaat
     5701 tacaaagcaa aaatcttaaa agcaaaagat atcgacactg ctgtttctgc tcaggtagta
     5761 ggtcatccag ttcgtgcctt aaaaaataaa ctagtaacaa cctatagcca agcagaaaaa
     5821 gattatttag ctggccgtat ttcaatcaat gagattgaag aattaggagc gggagctctt
     5881 cgcaatgcag ttgttgatgg ggatgttatt aatggttcag ttatggcagg acaaattgca
     5941 ggattaatta aatcagaaga gacttgtcaa gagattcttg aagatattta ttcaggagcg
     6001 cgtcaagtta ttctttctga ggcaagccgc tggtcagatt tataaataca aataaggaga
     6061 cactatgaat aaagtttcat ttctatttgc tggtcaaggt gctcaaaagt taggaatggc
     6121 tagagattta tatgaaactt tcccgattgt taaagaaact tttgataagg caagtcatgt
     6181 tttaggatat gatttgagag aattgattga taaagactta gacaaattaa atcagacgaa
     6241 atatacgcaa ccagctattc taacgacttc gactgctatt tatcgtttaa ttcttaaaga
     6301 aattgaactt agaccagata tggttgcagg actttctttg ggagaataca gtgctttagt
     6361 tgcttcaggt gctataagat ttgaagatgc tgtagtactt gttgctagac gaggccaact
     6421 aatggaagca gcagcacctg ctggtagtgg caaaatggta gcagttttaa atgctgatcg
     6481 tcaaataatt gaagatgctt gtaagaaggc ttctcagttt ggtattgtta gtcctgcaaa
     6541 ttataatact ccaaagcaaa ttgttatagg gggagagtca atcgctgtaa atgcagctgt
     6601 agaagaatta aaacaacaag gagtaaaacg tttaattcct ctgaatgttt caggtccttt
     6661 tcacactgct ttattgaaac ctgcaagtca gaaattgagc gatgttttag ataaagttca
     6721 tttttcagtt tctgaaatcc ctgtaattgg aaatacagag gcgcagataa tgaaaaaaga
     6781 cgatatcaaa tctttattgg cacgccaagt tatggaacct gtacgctttg atgagtctat
     6841 tgaaaccatg aaaaaaatgg gaatgacaca ggttgttgaa attggtccag ggaaggtact
     6901 ctcaggtttc ttgaaaaaaa tagatagctc attatcagta cattctgttg aagataaaat
     6961 tggatttaat aatcttaagg aattaaacta ggggaaaata tgcaattaaa agataagaac
     7021 atttttatta cgggttcaag tagaggaatc ggccttgcaa ttgcccatca atttgcacaa
     7081 ttaggtgcta atattgtttt aaatggacgt tctgaaatat cagaagactt aatagctgaa
     7141 tttgctgatt atggtgtaaa agttattgct atttcaggag atgtttcttc ttttgaagat
     7201 gctaatcgca tgattaaaga agctattgca agtctaggta gcgtggatgt gctcgttaac
     7261 aacgctggta ttactaatga taaattgatg cttaaaatga ctgttgaaga ttttgagagc
     7321 gttcttaaaa ttaatttaac aggtgcattt aatatgacac aatcagtctt gaaaccgatg
     7381 acaaaagcgc gtcaaggtgc tattattaat atctctagcg tcgttggatt aactggaaat
     7441 gtagggcaag ctaattacgc agcctctaaa gcaggtttaa ttggctttac aaaatcagtt
     7501 gccagagaag tagcagcacg aggcattcgt gtcaatgcca ttgctccagg ctttatcgaa
     7561 tctgatatga cagatgttat cccagaaaaa atgcaagaag ctattttagc tcaaattcca
     7621 atgaaacgta ttggaaaagg taaagaagta gcgcaggtag cgtctttctt agcagaacaa
     7681 gaatacctta caggacaagt tattgccatt gatggcggta tgacgatgca atgataaaga
     7741 ggaaataata tgacattaca gcgagtagta gtaacaggtt acggtgtaac ctcacctatt
     7801 ggaaacacac cagaagaatt ttggaatagt cttaaagaag gaaatgttgg tattggacct
     7861 atcactaaat ttgattcaag tgatttcatg gtcaaaaatg ctgctgaaat tcatgatttt
     7921 ccatttgata aatattttgt caaaaaagat ctgaacagat ttgatatgta ttccctttat
     7981 gccttatatg cttcaagtga agctattcaa catgctaatt taaatttaga tgaaattgat
     8041 gcagatcgat ttggtgtcat cgttgcatca ggtattggag ggattcaaga gattgaagaa
     8101 caagtgattc gtcttcatga aaaagggcca aaacgcgtaa aaccaatgac gcttccaaaa
     8161 gctctaccaa atatggcagc tggaaatgtt gcgatgaggt taggcgctca tggtgtatgt
     8221 aaatcaatta atacagcttg tgcttcttct aatgatgcta ttggagatgc tttccgtaat
     8281 ataaaatttg gtattcaaga tattatggta gtcggtggtg cagaagctgc tattacaaaa
     8341 tttgccattg ctggattcca aagtcttaca gcattatcaa caactgaaga tccatcaaga
     8401 gcatctattc catttgataa agatcgcaat ggatttatca tgggagaagg ttctggaatg
     8461 ttggtcttag aaagtttaga acacgctgaa aaacgaggag ctactattct tgcagaagtt
     8521 gttggttatg gtaacacttg tgacgcatat catatgactt caccacatcc agaaggttta
     8581 ggagccacta aagctattca acttgcactg gttgaagcta atattaaacc tgaagaagtt
     8641 aactatgtaa atgctcatgg aacttcaaca cctgctaacg aaaaaggaga gagtcaagct
     8701 attgttgctg cacttgggac agacgttcca gtttcttcga ctaaatcttt tactggtcat
     8761 cttttgggtg ccgcgggagc tgttgaggca attgcaacta ttgaagcgat acgtcatagt
     8821 tatgttccaa tgactgctgg aacgacagaa ttatcagaag atattacagc gaatgttatt
     8881 tttggtcaag ggcaagacgc tgatattcgt tatgccattt caaatacatt tggttttgga
     8941 ggacacaatg cagttctggc cttcaaacgc tgggaggatt aaattggata ttcaagagat
     9001 taaggattta atgactcaat ttgatgagtc aagtctgaga gaattttcat tcaaaacaag
     9061 tgacggtgag ctgtctttta gtaaaaatga gggaaaagct ccacttgtcc caactatgtc
     9121 tcctatgagt catcaacctg aagcgactcc aactatagca actcctgtct ctaatgaagc
     9181 aggtgagcaa acgaaacaag ctactgaagt agtttcagaa atcccagagt cgactgtgac
     9241 tgttgcagaa ggtgacgttg tcgaaagtcc tttagttggg gttgcttatt tagcttctgg
     9301 accagataaa cctaactttg tttcagtagg tgatagcgtg aagaagggac aaactttgat
     9361 gattattgag gcaatgaagg tgatgaatga agtgcctgcc ccccatgatg gtgtagttac
     9421 tgaaattctt gtagccaatg aagaagttat tgaatttggt aaaggattgg tacgaatcaa
     9481 atgattgata taaaagaaat tcgtgaggct ttacctcacc gctatccaat gttattagtg
     9541 gatcgtgttt tagaggtatc agaagatgag atagtcgcta tcaaaaatgt tagtatcaat
     9601 gagccttttt tcaatggtca ttttccagaa tatcctgtaa tgccaggcgt tttaattatg
     9661 gaagctttgg ctcaaactgc aggtgtttta gaattatcta aagaagaaaa caaaggaaag
     9721 cttgtttttt atgcaggtat ggataaagtg aaatttaaaa agcaagttgt tccaggagat
     9781 caattagtta tgactgctaa gtttgtcaaa cgtagaggaa ctatagccgt tgttgaagca
     9841 atagcagaag ttgatgggaa attagctgcc agtggaacgt taacatttgc aataggcaat
     9901 tgaagatagt attgaattta gagggagaga agcatcaaaa atgtttaaga aaatattgat
     9961 tgctaatcgc ggagaaattg cagtacgtat cattcgtgca gcccgtgaaa tgggaatttc
    10021 gacagttgct atctattcgg aggctgacaa ggaatcattg cacacaattt tagcagatga
    10081 agctatttgt gttggaccag ccaaatcagc agaatcttat ttaaacgtta atgctatatt
    10141 atctgctgct attgtgactg gagcggaagc tgttcatcca ggttttgggt ttttgagtga
    10201 aaattctaaa tttgctacta tgtgtgaaga gatgaactta aaattcattg gtccatctgg
    10261 agaagttatg gataaaatgg gtgataaaat caatgctcgt acagagatga taaaagctga
    10321 tgttccggtt atcccaggat cagatggtca agttacaagt gtagaagaag ctgtatcaat
    10381 tgctgaggaa atcggctatc cattgatgtt aaaagcctca gccggtggcg gtggaaaagg
    10441 aattaggaag gtcaaatcag cagatgaatt gaagcctgct tttgagtctg cttcacagga
    10501 ggctttggct gcgttcggta atggtgctat gtatattgaa aaagttatat acccagctag
    10561 acatattgaa gtacaaatac ttggagatag ctttggtaag atagttcacc taggagaacg
    10621 tgattgttct ctgcagcgta acaatcagaa ggtattggaa gaaagtccgt ctgtggcaat
    10681 aggtaacact ctgcgtcaac aaattggtga agcagctgtt cgtgcagctg aggctgtatc
    10741 ttatgaaaat gcaggaacaa ttgagttttt gttagatgag aatagcggac agttctattt
    10801 catggaaatg aatactcgtg ttcaagtaga acaccctgtt acagaatttg taacgggtgt
    10861 tgacatcgtt aaagagcaga ttcgtatcgc tgctggaatt cctttatcag tttctcaaaa
    10921 tgatattaag ttaactggtc atgcgattga atgtcgtatt aacgcagaga atccacaatt
    10981 taattttgcg ccatgtccag gtaccattaa tgggttacat ctacctgctg gcggtatggg
    11041 cttacgtgtc gattcagcgg tttacacagg ctacactatt cctccttatt acgatagtat
    11101 gattgcgaaa gtaattgttc acggagaaaa tcgttttgat gcacttatga aaatgcaacg
    11161 tgccttatat gagcttgaaa ttgatggtat tgtaacaaat acagaatttc aaatggatct
    11221 catttctgat aaaaaagtct tggctgggga ttatgatact tcattcctga tggaagactt
    11281 tttaccaagg tattatggta acaatcaata ggagggaaga tggctttatt tagtaaaaag
    11341 gataaatata tccgtattag tccaaacaaa gcattagggt cttctgataa acgtagtctt
    11401 cctgaggttc ctgatgaact ctttgctaag tgtccttctt gtaaacatat gatttatcag
    11461 aaagatttag gattagcaaa gatttgtcca gcttgttctt acaactttag aatttcagcg
    11521 caagaacgtc tgttgctaac agttgacgaa gatagttttg aggaattatt tacaggaatt
    11581 gaaactaagg atcctttgaa ttttccaaac taccgtgaaa aactggctgc tacacgtcaa
    11641 aagactaact tagatgaagc ggtggtgaca ggtttagcta aaataaaagg acaaactacg
    11701 gccttagcta ttatggattc tcattttata atggcgtcta tgggaacggt cgtaggcgaa
    11761 aagttgacaa gattatttga gctagcaact gaaaagaaat tacctattgt tatttttaca
    11821 gcgtcagggg gcgccagaat gcaagaaggt attatgagtt tgatgcaaat ggcaaaagta
    11881 tcggcagcag ttaaacgcca ttctaaccag ggattgtttt atttaacaat tttaacagac
    11941 ccaacaaccg gcggcgttac tgcgagtttt gctatggaag gtgatattat tctggctgag
    12001 ccacaagcgt tggttggttt tgctggtcgt cgtgttatcg aaacaacagt gagagaagat
    12061 ttacctgaag gctttcaaaa ggcagagttt ttgttagaac atggttttgt cgatgctatt
    12121 ataaatcgaa cagaattacg cgactgcatt gctcaactaa ttgctttcca tggaaatgag
    12181 gttggtcatg aataatgatg tgacaagaat tttaaaagat gcgcgtgatc aagggaggct
    12241 aactgctctt gactacgctg aattgatttt tgataatttt atggaacttc atggtgatcg
    12301 ccagttcgca gatgacaaat caattatagg aggtttgggg tacttggcag ggcgtcctgt
    12361 gacgattgtc ggtattcaaa aaggtaagaa ccttcaagat aatttagacc gtcattttgg
    12421 tcaaccacat cctgaaggtt atcgtaaggc tttacgttta atgaaacaag ctgagaagtt
    12481 tggaagacct gttataacct tcatcaatac tgctggagca tatcctgggg taggtgctga
    12541 ggagcgaggt cagggtgaag caattgctag aaacctctta gaaatgagtg atttaaaagt
    12601 ccctattata gctattatta ttggtgaagg gggttctgga ggagcattag ctttagctgt
    12661 tgccgataag gtttggatgt tggaacatac tgtttattct attttaagtc ctgagggctt
    12721 tgcctctatt ttatggaaag atggtacaag aacaacagaa gcagcacaat taatgaagat
    12781 gactgctgga gaactttacc atatggaagt tgtagacaaa gtaatccctg aacatggtta
    12841 tttttcaagt gagattgtcg atatgattaa gacaagttta atttcagagt tagaagtgct
    12901 ctcacaatta tctcttgagg atttattgga acaacgttac caacgtttta gaaaatatta
    12961 atagaaaaag gctgagttat ttactcagcc tatattt
//
LOCUS       DATRMA010000051        12982 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.051, whole genome shotgun sequence.
ACCESSION   DATRMA010000051 DATRMA010000000
VERSION     DATRMA010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12982
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.051"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            80..931
                     /gene="ylqF"
                     /locus_tag="VBC28_001416"
     CDS             80..931
                     /gene="ylqF"
                     /locus_tag="VBC28_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HEO6815396.1"
                     /translation="MATIQWFPGHMSKARRQVQENIKHVDFVTILVDARLPLSSQNPM
                     LTKIVGDKPKLMILNKADLADPIRTKEWRDFYESQGLKTLAINSKEQSTVKKVTDIAK
                     ILMSDKIAKLRGRGIQKETLRTMIIGIPNAGKSTLMNRLAGKKIAVVGNKPGVTKGQQ
                     WLKSNKELEILDTPGILWPKFEDELVGLKLALTGAIKDQLLPMDEVTIFGLNYFKTYY
                     PDRLKERFKSINLEDEAPEIIMALTQKLGYRDDYDRFYNLFVKEVRDGKLGRYTLDIV
                     GEHDGNN"
     gene            918..1679
                     /locus_tag="VBC28_001417"
     CDS             918..1679
                     /locus_tag="VBC28_001417"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEO6815397.1"
                     /translation="MATIKEIKAILETIVDLKDKRWQEYQTDSRAGVQKAILQRKKNI
                     QSDLDEEARLEQMLVYEKKLYIEHINLIAGIDEVGRGPLAGPVVAAAVILPPNCKIKH
                     LNDSKKIPKKKHQEIYQNILDQALAVGIGIQDSQCIDDINIYEATKHAMIDAVSHLSV
                     APEHLLIDAMVLDLSIPQTKIIKGDANSLSIAAASIVAKVTRDKIMSDYDSTYPGYAF
                     SKNAGYGTKEHLEGLQKYGITPIHRKSFEPIKSML"
     gene            1700..2305
                     /locus_tag="VBC28_001418"
     CDS             1700..2305
                     /locus_tag="VBC28_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HEO6815398.1"
                     /translation="MTEKEKMLAGQYYRPSAPELRKDREVALKNMQAFNNEDNSSKRN
                     VILQKWFGATGKSIHMEQRFVCDYGCNIYVGENFYANFNQTFLDVCEIRIGDNCMFGP
                     NCQLLTPLHPLDPIERNSGLEYGAPIQIGNNVWLGGGVTILPGVVLGDNVVVGAGSVV
                     TKSFENNVVIAGNPAKIIKKISLKLEKPLIITKVFSNFKNR"
     gene            2487..3449
                     /locus_tag="VBC28_001419"
     CDS             2487..3449
                     /locus_tag="VBC28_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEO6815399.1"
                     /translation="MKKLLTTPVMGCTLVILSLTSLFVGVKSIPLEQITHLDQSQVDI
                     FLTSRLPRTISILISGASLSVCGLLMQQLTQNKFVSPTTSGTMDWAKLGVVVTLIFFK
                     NTSIFIQLCIASGFAILGSLLFVTILKMITFKDNIFIPLIGLMLGQIVAAATVFLGTH
                     FQVLQSVNSWLQGNFSIMTSHRYEILYLALPCLFLVYFFAHQFTIVGLGESFAKNLGV
                     AYEKMIYFGLVLVSIMTSLVIIIVGALPFLGLIVPNLISITKGDHMSSTILETSLLGA
                     CIVMICDLFGRLVIFPYEVSIGVTLGVLGSAFFLISIIRNEGKS"
     gene            3446..4420
                     /locus_tag="VBC28_001420"
     CDS             3446..4420
                     /locus_tag="VBC28_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEO6815400.1"
                     /translation="MIKDLEIPKLLILLILLIAAIILFLIYGIPTDANEFLIIYILKT
                     RYQKLIALILVGICIGSSSLIFQTLTNNRLLTPSIIGLDSLYILIQTGLMYLIGAQRV
                     IKFSSFSSFLLSLLLMVGFAYLLFTILFRNKKQSLYFVLLAGLIFNTLFSSISSFIQA
                     IMDPNDFMILQNQLFASFNAINTKILWISFIIIVVSFVINWPFIKELDVLLLGKENAI
                     SLGISYQKLTTRFFLWLALMVAIATALVGPITFLGLLVAHITYHSFHTFRHQILVPIA
                     IVICIFTLVLGQHLVQNLLHLTVQLSVLLNLIGGSYFIFTLIKGRKNL"
     gene            4417..5178
                     /locus_tag="VBC28_001421"
     CDS             4417..5178
                     /locus_tag="VBC28_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000614752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO6815401.1"
                     /translation="MIQINNLHKFYGQKEILKDINISIPKGKVTAILGPNGSGKSTLL
                     SCISRLEPYDNGEIFLDKVPLAHYSSNDLAKTLAILRQSNHLTLKIKVRDLIGFGRFP
                     YSKGRLSQKDKAVIESVISYMDLNDIADEFINNLSGGQIQRAFIAMTMAQDTQYICLD
                     EPLNNLDMKYAVQMMDLIKRYAYEFNKTIVIIIHDINFATHYADNVVALKEGQVVTCG
                     TVEDVMQEKILSHLFDMPIRIETVDGKPIILFCTT"
     gene            5240..6268
                     /locus_tag="VBC28_001422"
     CDS             5240..6268
                     /locus_tag="VBC28_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HEO6815402.1"
                     /translation="MTKKLIIAILALCTILTTSQAVLAKEKSQTVTIKNNYSVYIKKE
                     KRDKPDNKKQISETLKVPLKPKKVVVFDMGALDTITALGAEKSVIGIPKAKNALSLLP
                     NNVKSVYKAKRYQDVGSLFEPNFEAIARMQPDVVFLGARMASVDNIEKLKEAAPKAAL
                     VYAGVDSKKVFDKGVAERVTMLGKIFDQNKKAKTFNKDIAQAVLKLQKTIEKKGKPTA
                     LFVMANSGELLTQSPSGRFGWIFSVGGFKAVNENEKLSSHGTPVSYEYIAEKNPNYLF
                     VLDRGATIGQGASSKELFNNDVIKATDAVKNKRVHEVDGKDWYINSGGSRVTLRMIKD
                     VQNFVDNR"
     gene            6406..7248
                     /gene="dprA"
                     /locus_tag="VBC28_001423"
     CDS             6406..7248
                     /gene="dprA"
                     /locus_tag="VBC28_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001015242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HEO6815403.1"
                     /translation="MNHFELFKLKKAGLTNLNINNIINYLKKNSLTSLSVRNMAVVSK
                     CKNPTFFIENYKQLDLKKLRQEFKKFPVLSILDSNYPLELKEIYNPPVLLFYQGNIEL
                     LSKPKLAVVGARQASQIGCQSVKKIIKETNNQFVIVSGLARGIDTAAHVSALKNGGSS
                     IAVIGSGLDVYYPTENKKLQEYMSYNHLVLSEYFTGEQPLKFHFPERNRIIAGLCQGI
                     VVAEAKMRSGSLITCERALEEGREVFAIPGNIIDGKSDGCHHLIQEGAKCIISGKDIL
                     SEYQ"
     gene            7343..9463
                     /gene="topA"
                     /locus_tag="VBC28_001424"
     CDS             7343..9463
                     /gene="topA"
                     /locus_tag="VBC28_001424"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEO6815404.1"
                     /translation="MATTTKTSTKKTSKKKSATAKKNLVIVESPAKAKTIEKYLGRNY
                     KVVASVGHIRDLKKSSMSIDFENNYEPQYINIRGKGPLINDLKKEAKKAKKVYLASDP
                     DREGEAISWHLAHILDLDKEDRNRVVFNEITKDAVKNAFVEPRQINIDLVDAQQARRV
                     LDRIVGYSISPILWKKVKKGLSAGRVQSVALKLIIDRENEIKAFQPEEYWTIDGSFKK
                     GTRKFNATFYGLDGKKFKLSNNEDVKTVLKRIKTDEFLVEKVEKKERRRNAPLPYTTS
                     SLQQDAANKINFRTRKTMMIAQQLYEGLSLGTAGHQGLITYMRTDSTRISPLAQNEAT
                     EFITNRFGANYSKHGNKVKNASGAQDAHEAIRPSSVNHTPESIAKYLDKDQLKLYTLI
                     WNRFIASQMTAAVFDTMKVNLTQNGVTFIANGSQVKFDGYMAVYNDTDKNKMLPDMEE
                     GESVKKVNTNPEQHFTQPPARFSEASLIKTLEENGVGRPSTYAPTLETIQKRYYVKLA
                     AKRFEPTELGEIVNSLIVEFFPDIVDVTFTAEMEGKLDEVEIGKEQWQKIIDEFYKPF
                     EKELAKAETEMEKIQIKDEPAGFDCELCGSPMVIKLGRYGKFYACSNFPECHNTKAIT
                     KEIGVICPICQKGQVIERKTKRNRIFYGCDRYPECEFTSWDKPIGRTCPKSNDFLVEK
                     KVRGGGKQVVCSNEKCDYQEEKIK"
     gene            9600..10301
                     /locus_tag="VBC28_001425"
     CDS             9600..10301
                     /locus_tag="VBC28_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6815405.1"
                     /translation="MSYIEMKHSYKRYQTGETEIVANNDISFSIERGELVVILGASGA
                     GKSTVLNILGGMDSNSEGEVLIDGKNIANYTIRELTRYRRYDVGFVFQFYNLVPNLTA
                     LENVELASEIVPKALDAQQALENVGLGHRINHFPAQLSGGEQQRVAIARAIAKKPKLL
                     LCDEPTGALDYQTGKQVLAILQKMAQSKETTVIIVTHNTALAPIANRVIHMHDSKISD
                     IVINENPSDIQNIEY"
     gene            10313..12943
                     /locus_tag="VBC28_001426"
     CDS             10313..12943
                     /locus_tag="VBC28_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000520608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6815406.1"
                     /translation="MGKTFWKDIYRSITTSKGRFSSILLLMMLGSFAFIGLKVSAPNM
                     QRTAQNYLAHHHVMDITVFNSWGLDKHDQTVLESLKGSQVEFSYFVDTTPQQNSKSYR
                     LYSNTKTISTFDLVKGRLPLNKSEIALSFQERKKYAIGDKINFKQDKNKLFSNTGPLT
                     IVGFVNSTEIWSKTNLGSSQTGDGDLDSYGVLDKTAFHSPVYTMARVTFKDLRLINPF
                     SISYKEKVAKYQEKVSRKLNIHNKIRYTKTKKESLRKIDEEEKSLLKAQKQINRLDND
                     SLAMPLSQRQAIQMKIKQDRLSLLKRTKELLKLRHNTQIMESPQIIVYNRTTFPGGQG
                     YNTFDSSTNSTSKISNLFPIILYLVAALVTLTTMTRFVEEERTNAGILKALGYSDRQV
                     IFKFIIYGFIAGTLGTTLGIIGGHYLLPRIISDIISKDLTIPNTQYHLFLNYSLLAFV
                     FSLLSIVLPVFVITRRELKEKAAFLLLPKPPAKGSKIALEYINWIWKKLSFTQKVTAR
                     NIFRYKQRMIMTIFGVAGSVALLFSGLGIQSSLKQTVNEHFGRIMPYDILLTYNTNAS
                     PPKILELLSKDSKIDKYQPIHLENLDESIPGQINKQSISLFITDKKQLLPFIYLQEAT
                     TNKSLHLNNKGIIISKKLAQFYHVNTGDFIHLSHSQTLPSRKLKITGVVNANVGHYIF
                     MTKQYYRTIFKKEAKDNAFLVKLTKHKIANNLAEKLLEINGVESLTQNALQLASVEAV
                     VRSLDGSMTILVVVSLLLAIVILYNLTNINLAERKRELSTIKVLGFYNEEVTLYIYRE
                     TIILSTIGVILGTISGTYLHRQMMLLIGSDQILFGEKVSPTTFIIPISVVVIILISLG
                     FIVNHQLKKLNMLDALKSVD"
BASE COUNT         4497 a         2150 c         2248 g         4087 t
ORIGIN      
        1 gaataaaact aaaaaattag acctctaaca aatagatgta gacttttaag gtagtctata
       61 cataaggaga atttatttca tggctactat tcaatggttc ccaggacaca tgtccaaagc
      121 tcgccgtcaa gttcaagaaa atattaagca tgttgatttt gttacaattt tagtggatgc
      181 aaggttacct ctttctagtc aaaatccaat gctaaccaaa atcgtcggag acaaacccaa
      241 actaatgatc ctcaataaag ctgatctagc agatcctatt cgaactaaag aatggagaga
      301 tttttatgaa tctcaaggtt taaagacatt agctattaat tctaaagaac aatcaacagt
      361 taaaaaagta accgacatcg ctaaaatatt aatgtccgat aaaattgcta agctacgggg
      421 acgtggtatt caaaaagaga cacttcgtac gatgattatt ggaattccga atgcaggtaa
      481 atcgactcta atgaatcgcc tagcaggtaa aaaaattgca gtggttggta ataaaccagg
      541 tgtcacaaaa ggtcagcaat ggctcaaatc taataaggaa ctggaaattt tggacactcc
      601 tggtatcttg tggcccaaat ttgaagatga attagttgga ttaaagctag cattaacagg
      661 ggcaatcaaa gatcaattat tgcctatgga tgaggtaaca atatttggat tgaattactt
      721 taaaacctat tatcctgatc gtctcaaaga gcgttttaaa agcattaatt tagaggatga
      781 agctcctgaa atcatcatgg ccttaacaca aaaattaggt tatcgtgacg attacgatcg
      841 cttttataat ttatttgtca aagaagttcg cgatggtaaa ttaggacgct atacgttaga
      901 tatcgtcgga gaacatgatg gcaacaatta aagaaatcaa agcaatttta gagactattg
      961 ttgatttaaa agacaaacgt tggcaagaat atcaaacaga ttcacgcgca ggtgttcaaa
     1021 aagcaatcct tcaacgaaaa aaaaatattc agtcagattt agacgaagaa gcgcgcttag
     1081 aacaaatgtt ggtctacgaa aaaaaacttt acattgaaca tataaacctt attgcaggaa
     1141 tcgatgaagt aggtagaggt cctttagcgg gaccagtagt agctgctgct gttattcttc
     1201 caccaaactg taaaataaaa catctcaatg actccaaaaa aattccaaag aaaaagcatc
     1261 aagaaatcta tcaaaatatt ttagatcaag cgttagcagt tggtattggt attcaagaca
     1321 gccaatgtat tgatgatatc aacatttatg aagctacaaa acatgctatg attgatgcag
     1381 tttctcattt atctgtggct cctgaacatc ttttaattga tgctatggtt ttagatctat
     1441 caattccaca aactaaaatt atcaaaggtg atgctaattc actctctatt gcagcagctt
     1501 caatcgtagc taaagtgacc agagataaaa taatgtctga ttatgatagc acatatcctg
     1561 gatatgcatt ttctaaaaat gctggctatg gtactaaaga gcatttagaa ggtttgcaaa
     1621 aatacggtat tactccgata catcgtaaaa gttttgaacc tataaaatca atgttgtaaa
     1681 taatacggaa gaggaaaaga tgaccgaaaa agaaaaaatg ttagcaggac aatattatcg
     1741 tccatcagca cctgaactac gcaaggatag agaagttgca ctaaaaaata tgcaagcctt
     1801 taacaatgaa gataatagta gcaaacgtaa cgtcatttta caaaaatggt ttggtgctac
     1861 tgggaagagt atccatatgg aacaacgttt tgtctgcgat tatggttgta atatttatgt
     1921 tggtgaaaac ttctacgcca attttaacca aactttctta gatgtttgtg aaattcgtat
     1981 tggcgataac tgtatgtttg gcccaaactg tcaactcctc accccactcc atcctctaga
     2041 tccaatagaa agaaattcag gattagaata cggtgcccca atccaaatcg ggaataatgt
     2101 ctggctaggt ggaggagtga ctattttacc aggtgttgta ttaggagata atgtcgtcgt
     2161 tggcgctgga agtgtcgtta cgaaatcatt tgaaaataac gttgttatag caggaaaccc
     2221 tgctaagata atcaaaaaaa ttagcttaaa attagaaaaa cctttaatta taacgaaggt
     2281 tttttctaat tttaaaaata ggtaaagtaa atattctgct aatttaatct atcttattta
     2341 taatgatttt aaatagaaaa accttgatat aataatgttt tcttaagtat aaaattcttt
     2401 aaaaagtaga tatttttcca aaaaattatg ttattatagt tttgtgctca tttttgaaca
     2461 ctttatcttt cattttaagg agttttatga aaaagctatt aaccactcct gtaatgggtt
     2521 gtactttggt tatactttct ctaacgtccc tatttgttgg tgtgaaatct atcccactgg
     2581 agcaaataac acaccttgat caatcacagg tagatatttt tttaacgagt cgcttaccaa
     2641 gaaccattag tatcctaata tctggtgcaa gtttgtcagt ctgtggcctg ctgatgcaac
     2701 aactaaccca aaacaagttc gtttcaccga caacttcagg gactatggat tgggcgaaat
     2761 taggagtagt tgttacgctg attttcttta aaaatacctc tatttttatt caactatgta
     2821 tagcttcagg ttttgccatt ttaggatcgc ttctttttgt aaccatttta aaaatgatta
     2881 cttttaagga taatattttt atacctctta tcggactgat gcttggacaa attgtagcag
     2941 cagcaacagt atttttagga acacattttc aagtactaca atctgtcaac agttggcttc
     3001 aaggtaattt ttccatcatg actagccatc gctacgaaat tctttactta gcattgccat
     3061 gtttgttttt agtatatttt tttgctcatc agtttaccat tgttgggctt ggagaaagct
     3121 tcgctaagaa tttaggggtt gcttatgaaa agatgattta ctttggttta gttttggttt
     3181 caattatgac atccctagtc attattatcg taggagcttt acctttctta ggtttaatcg
     3241 tacctaatct aattagcata actaaaggag atcatatgtc ctcaactatc ttagagacat
     3301 cgcttttagg ggcttgtatc gtcatgatat gtgatctttt tggtcgctta gttatttttc
     3361 cttatgaagt ctcaatagga gttacgctag gagtattagg tagtgccttt ttcctgattt
     3421 caattataag aaatgaggga aaatcatgat taaggattta gagataccaa aattacttat
     3481 tttactgata ctactaattg ctgctattat tttgttttta atttatggta ttccaactga
     3541 cgcaaatgaa tttttgatta tctatatcct aaaaacacgt tatcaaaaac tgattgcatt
     3601 aattttggtt ggtatctgca ttggtagttc tagtcttatt tttcaaactc taaccaataa
     3661 ccgactttta actccgtcca ttattggttt agatagctta tatatcctta tccaaacagg
     3721 attaatgtat ttaatcggag ctcagcgtgt cataaaattt tcatctttta gtagcttcct
     3781 cctttcatta ttattaatgg ttggatttgc ttacttatta tttacgatac tttttcgaaa
     3841 taaaaaacag tctttgtatt ttgttctatt agctggactc atatttaata cattattttc
     3901 ttctatctca tcctttattc aagccattat ggaccccaac gattttatga ttttacaaaa
     3961 tcagctcttt gctagtttca atgctattaa caccaagatc ctttggattt ctttcattat
     4021 cattgtcgtt agctttgtca ttaattggcc ttttataaag gaattggatg tcttactatt
     4081 gggtaaagaa aatgcgatat cactcggcat ctcatatcaa aagcttacta ctagattttt
     4141 tttatggtta gcattgatgg tagctatagc aacagcatta gttgggccga ttactttttt
     4201 aggcttactt gtagcacata ttacctatca cagttttcat acctttagac atcaaatatt
     4261 agttcctata gccattgtta tctgtatctt tacacttgtc ttagggcagc atctcgttca
     4321 aaatctatta cacctcactg ttcaactaag tgttctcctc aaccttatcg ggggaagtta
     4381 ttttatattc accttaatca aaggaaggaa aaacttatga ttcaaataaa caatttacat
     4441 aagttctatg gccaaaagga aatattaaaa gacattaata tttcgattcc taaaggaaaa
     4501 gtaacagcta ttttaggtcc aaatggatct ggaaagagta cattgctgag ttgtatcagt
     4561 cgacttgaac cttatgataa tggtgaaatt ttccttgata aagtaccatt agcccactac
     4621 agttcaaatg acttagccaa aacattagcc atattaagac aatctaatca cttaacactc
     4681 aagataaagg tgagagactt gataggattc ggtagatttc cttatagtaa aggacggtta
     4741 agtcaaaaag ataaagctgt catagaatca gttatatctt atatggattt aaacgatata
     4801 gctgacgagt tcattaataa cctcagcgga ggtcaaattc aaagagcttt tatcgcaatg
     4861 acaatggcac aagatactca atatatttgc ttagatgagc cactcaacaa tttagatatg
     4921 aaatatgctg ttcaaatgat ggatcttatc aaacgatatg cgtatgagtt taataaaact
     4981 atagtgatta ttattcacga catcaatttt gcaactcact acgctgacaa tgttgtcgct
     5041 ttaaaagaag gacaagtggt gacttgtggg actgtcgaag atgttatgca agaaaaaatc
     5101 ctttctcatt tatttgatat gcctatccgt atcgaaactg ttgatggtaa accaattatc
     5161 cttttttgta caacgtaaat aaagagattt atgtaggata acatttttag tttttatcta
     5221 ttttaggaga ttttatttaa tgacaaaaaa acttattatt gctatattag cactatgcac
     5281 tatcttaacc acttctcaag ctgttttagc taaagaaaaa tcacaaactg ttaccataaa
     5341 aaacaactat tcggtctata ttaaaaaaga aaaaagagac aagccggata ataaaaagca
     5401 aatcagcgag acacttaaag ttcctttaaa acccaaaaaa gtagttgttt ttgatatggg
     5461 agctttggat actatcacag ctttaggagc tgaaaaatct gttattggta tcccgaaggc
     5521 taaaaatgct ctaagtttat tgcccaataa cgtcaaatct gtttataaag ctaagagata
     5581 ccaagacgta ggaagtctct tcgaaccaaa ctttgaagct attgctcgta tgcaacctga
     5641 tgtggttttc ctaggagcac gtatggcttc tgttgataat attgaaaaat taaaggaggc
     5701 tgcacctaaa gcagcattag tatatgctgg agtcgactca aaaaaagtat ttgacaaagg
     5761 agttgctgag cgtgtcacaa tgttagggaa aatcttcgac caaaataaaa aggcaaaaac
     5821 ctttaataaa gatatcgcac aagctgttct taaattgcag aaaactattg agaaaaaagg
     5881 taaacctaca gctctatttg taatggcaaa cagcggtgaa cttttaactc aatcaccttc
     5941 tggtcgtttt ggttggattt tctctgtagg tggatttaaa gcagtcaatg aaaatgaaaa
     6001 actaagttca catggtactc ccgtatctta tgaatacatc gctgaaaaaa atcctaacta
     6061 tctctttgtt ttagatcgtg gagcgactat tggacaagga gcttcatcaa aagaactttt
     6121 taataacgat gttattaaag caactgatgc tgtcaaaaac aaacgtgttc atgaggtaga
     6181 tggaaaagat tggtatatca attcaggcgg aagccgagta acactccgta tgattaaaga
     6241 tgtacagaac tttgttgata atcgttaatt ttttgtcaaa ggctaggaaa aatcctagtc
     6301 ttattttttt gaaaaaatag ctattatcaa tgcttcagag tatagattga tcttatcact
     6361 ttattttgtc tattattttc gaatatagtc aatggaggta accttatgaa tcattttgaa
     6421 ctatttaagc ttaaaaaagc tggattaaca aatcttaata tcaacaatat tatcaactat
     6481 ctcaaaaaga atagtttaac ttctctatct gttcgcaata tggccgtagt atcaaaatgt
     6541 aaaaatccta ctttctttat agaaaattat aaacagctag accttaaaaa acttcgacaa
     6601 gaattcaaaa aatttccagt tctatcgatt ttggattcta actatccttt agagttaaaa
     6661 gaaatatata atccaccagt tctacttttt tatcagggta atattgaact tctatctaaa
     6721 cctaaattag ctgtagtggg ggcaagacag gcatctcaga taggttgtca gtctgttaaa
     6781 aagattatca aggagactaa caatcaattt gttatcgtaa gtggtttagc gcgtggcatt
     6841 gatacagcag cacatgttag tgctttaaaa aatggcggca gtagtatagc tgttatcggg
     6901 agtggtttag atgtttatta tccaacggag aataagaaac ttcaagaata tatgtcatat
     6961 aatcatctcg tattatcaga atattttacc ggagaacaac ccttgaaatt tcatttcccc
     7021 gaacgtaacc gtattattgc agggctgtgt caaggtattg tggttgccga agctaagatg
     7081 agatctggaa gtttaattac ctgtgaaaga gcattagaag agggacgaga agtttttgcc
     7141 attcccggaa atattatcga tggcaaatca gatggatgcc accatcttat ccaagaagga
     7201 gctaaatgca ttatttcagg aaaagatatc ctttctgaat atcagtagtg ttttgctttc
     7261 tactatagga gaagttttct attgacagtt taagaaaagt gagttattat gctataagtt
     7321 tacgatatag aaagtgatac ctatggcaac tacgacaaaa acgtcgacca aaaaaacaag
     7381 taaaaaaaaa tcagctactg ctaaaaaaaa tctagttatt gtggagtctc ctgcaaaagc
     7441 aaagactatc gaaaaatact taggacgtaa ctataaagtt gtagcctcag taggtcatat
     7501 ccgtgattta aaaaagtcaa gtatgtccat tgattttgag aataattacg aaccacaata
     7561 tattaatatt cgtggtaaag gacctctcat taatgattta aaaaaagaag ctaaaaaggc
     7621 taaaaaagtt tacctcgcga gtgacccgga ccgtgaagga gaagctattt cctggcattt
     7681 agcgcatatt ttagatttag acaaagaaga cagaaaccgt gttgttttca atgaaatcac
     7741 aaaagatgct gttaaaaatg cttttgtgga acctcgtcag attaacatag atctcgttga
     7801 tgctcagcaa gcacgacgtg ttttagatcg cattgttggt tattctatct cacctatttt
     7861 atggaaaaaa gtaaaaaaag ggctatcagc tggacgtgta caatcagttg ctcttaaact
     7921 aatcattgat cgtgaaaatg agattaaggc tttccaaccg gaggagtatt ggactattga
     7981 cggttccttt aaaaaaggaa cacgtaaatt caatgccact ttctacggtt tagacggaaa
     8041 gaaattcaaa ttatcgaata atgaagacgt caaaacagtt ctaaaacgta ttaaaactga
     8101 tgaattctta gttgaaaaag ttgaaaaaaa agagcgtcgt cgtaatgcac cattaccgta
     8161 tacaacttct tcattgcaac aagatgcagc taataaaatc aattttcgaa ctcgtaaaac
     8221 catgatgatt gcgcaacagc tgtacgaagg acttagctta ggcacagcag gtcatcaagg
     8281 tctgattacc tatatgcgta ctgattctac acgtattagt ccgttagcac aaaatgaagc
     8341 aacagaattt attactaacc gttttggtgc aaattattct aaacatggga ataaagttaa
     8401 aaatgcttct ggagcacaag acgctcacga agctattcgc ccatctagtg ttaatcatac
     8461 acccgaaagt attgctaagt atttagacaa agatcaacta aaactttaca ctcttatctg
     8521 gaaccgtttt attgcaagcc aaatgacagc agcagtcttt gacacaatga aagttaattt
     8581 aacgcaaaat ggtgttactt ttattgctaa tggtagccaa gtcaagtttg atggttacat
     8641 ggctgtttat aacgatactg acaaaaataa aatgttacca gatatggagg aaggagaaag
     8701 tgttaaaaag gttaatacga atcctgaaca acacttcact caacctcctg caaggttttc
     8761 agaagcaagt ctcattaaaa cacttgaaga aaacggtgta ggtcgtcctt caacttatgc
     8821 cccaacgctt gagactattc aaaaacgtta ttatgttaag cttgcagcta aacgcttcga
     8881 accaactgaa cttggtgaaa ttgttaatag ccttattgta gaatttttcc cagatattgt
     8941 tgatgttacc ttcactgccg aaatggaagg gaaactagac gaagttgaga ttggtaaaga
     9001 gcagtggcaa aagattatcg atgaatttta taaaccattt gaaaaagaac ttgcaaaagc
     9061 agaaactgaa atggagaaaa ttcaaataaa ggatgaacct gctggatttg attgcgagct
     9121 atgtggatca ccaatggtaa taaaactagg acgttatgga aagttttatg cgtgtagcaa
     9181 tttccctgaa tgtcataaca caaaagctat cactaaggaa ataggtgtta tttgtcctat
     9241 ctgtcaaaaa ggacaagtta ttgagcgaaa aacaaaacgc aatcgtatct tttatggctg
     9301 tgaccgctac ccagaatgtg agtttacatc ttgggacaaa cctattggac gaacttgccc
     9361 caaatcaaat gatttcttag tggagaaaaa agtacgcggt ggtggtaaac aagttgtttg
     9421 ctcaaatgaa aaatgcgact accaagaaga gaaaattaaa taaagtggaa tctgagtgtt
     9481 tagctcagat ttttaaaaaa attattattt tacataactt aatttatgta tttattttta
     9541 tgggagaatt aataaaaaag tgctataata taggtgtatt catagtgtga ggtgccgtta
     9601 tgtcctatat tgaaatgaag cattcctata agcgttatca aacaggtgaa accgaaattg
     9661 ttgctaacaa tgatattagt ttcagtattg aaagaggtga gttggtggtt attcttggtg
     9721 catccggcgc tggcaaatct actgttctca atatactagg agggatggat agtaatagtg
     9781 aaggtgaagt tttaattgat ggtaagaata ttgctaatta taccatcagg gaattgactc
     9841 gttaccgtag atatgatgtc gggtttgttt ttcaattcta taacttagtc cctaatctaa
     9901 cagcactaga gaatgtagaa ttggcttcag aaattgttcc aaaggcatta gatgcccaac
     9961 aggcattaga aaacgtaggt ttgggacacc gtatcaatca ttttccagca cagctttccg
    10021 gtggcgaaca gcaacgcgtt gcaatagcac gagctattgc caaaaaacct aaactgttac
    10081 tttgtgatga acctactggt gctttagatt accaaactgg aaaacaagta ctagccatcc
    10141 tgcaaaagat ggcgcaatca aaagaaacaa ctgtgatcat tgttactcat aatactgcct
    10201 tagcgccaat tgcaaatcgc gtgatccata tgcatgattc aaaaatcagt gatattgtaa
    10261 taaacgagaa tccaagtgat attcagaaca tagaatatta gaggttatac agatgggaaa
    10321 gacattttgg aaagatatct atcgatcaat tacgacttca aaaggtagat tttcatcaat
    10381 cctcttactt atgatgttag gctcatttgc ttttatagga ctgaaggtct cagcacctaa
    10441 tatgcaaaga acagcacaaa attaccttgc tcatcatcat gttatggata ttaccgtttt
    10501 caattcttgg ggacttgaca aacatgatca aactgtttta gaaagcttaa aaggatcaca
    10561 agttgaattt tcttactttg tagatacaac ccctcaacag aatagtaaat cttatcgatt
    10621 gtattccaat accaaaacga tatcaacctt tgatcttgtt aagggacgtc tccctttaaa
    10681 taaatcagaa attgctctct catttcagga acgaaaaaaa tatgcaatag gtgataaaat
    10741 taattttaaa caagataaaa acaaactgtt ttcaaataca ggtcctctaa ctattgtagg
    10801 ttttgttaat tctaccgaga tttggtctaa gacaaacctt ggtagttctc aaacagggga
    10861 cggcgactta gatagttatg gcgtattaga taagacagca tttcactctc cagtatatac
    10921 aatggcaagg gtaactttta aagatcttag gttgattaac cctttctcaa taagttacaa
    10981 agaaaaagta gcgaaatacc aagaaaaggt atcgcgaaaa ttaaatattc acaataaaat
    11041 aagatatacc aaaacaaaaa aagagagcct acgtaaaatc gatgaggagg aaaaaagcct
    11101 actaaaagct caaaaacaaa tcaatcggtt agataatgat agcctagcga tgccactatc
    11161 tcaaagacaa gctattcaaa tgaaaattaa acaagaccga ctatctcttt tgaaacgaac
    11221 aaaagagctt ctaaaattga gacataacac ccaaataatg gaatcgcctc aaattattgt
    11281 ctacaaccgt actacctttc ctggaggaca aggatataac acttttgact ccagtacaaa
    11341 tagcacttct aaaatcagta atcttttccc cattatttta tatttagtcg cagcattagt
    11401 aaccttaaca acaatgacta ggttcgtcga ggaagaaaga accaacgcag gcatattaaa
    11461 agcactaggc tatagtgacc gccaagttat ctttaaattt attatttatg gttttatagc
    11521 aggaacatta gggactactt tgggtatcat tggagggcat tatcttttac ctcgtattat
    11581 ttctgatatc atttccaaag acctgactat tccaaacaca cagtatcatc ttttcttaaa
    11641 ttacagttta ctagcctttg ttttttcatt attaagcatt gttcttccag tttttgttat
    11701 cactcgacgt gagttgaaag aaaaagcagc atttttatta ttaccaaaac caccagctaa
    11761 aggatctaaa atagctttag agtatatcaa ttggatttgg aaaaaactgt ccttcacaca
    11821 aaaagtaact gctagaaata tttttcgtta caaacaaagg atgattatga ccatatttgg
    11881 agttgctgga tcagtagcgc tcttattttc aggtctagga atacaatcct ccttaaagca
    11941 aaccgtcaat gaacatttcg gtcgtattat gccctatgat atattgctaa catataacac
    12001 aaatgcttct cccccaaaaa tacttgaact actttcaaaa gattcaaaaa tagacaaata
    12061 ccaaccaata caccttgaaa atcttgacga atccattcct ggacaaatta ataaacagtc
    12121 aatttcccta ttcataacag acaaaaaaca attactacct tttatttatt tacaagaagc
    12181 aacaactaat aagtcattgc acttgaataa taaagggatt attatttcta aaaaattagc
    12241 tcaattttat catgttaata ccggtgattt tatccattta tctcattcac aaacacttcc
    12301 ttctagaaaa ttaaaaataa caggagttgt caatgcgaat gttggtcact atatttttat
    12361 gacaaaacaa tactatcgaa ctatttttaa gaaagaagct aaagataacg cttttttggt
    12421 taagttaacc aaacataaaa tcgcaaataa cttagcagaa aaacttttag aaattaatgg
    12481 agttgagtct cttacgcaaa atgctcttca gctggctagt gtagaagccg ttgtgcgttc
    12541 tttagatggc tctatgacta ttttagttgt cgtatcttta ttgttagcca ttgtaattct
    12601 ctataatctc actaatatta atttagcaga acgtaagcgg gagctatcaa ctataaaggt
    12661 attaggtttt tataatgaag aagtaacttt atacatttac cgcgaaacca ttattttatc
    12721 aaccatcggt gtgattctag gcaccattag cggtacttat ttacaccgtc aaatgatgtt
    12781 gctaattggt tcagatcaaa tactttttgg tgaaaaagta tcaccaacta catttataat
    12841 accaataagc gtagtagtca tcattctaat aagtctaggt tttatagtta accatcaatt
    12901 aaaaaaactc aatatgttag atgccttgaa atcagtagat tagtttataa taaagattat
    12961 cttttctaat cttttcgata ac
//
LOCUS       DATRMA010000052        12871 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.052, whole genome shotgun sequence.
ACCESSION   DATRMA010000052 DATRMA010000000
VERSION     DATRMA010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12871
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.052"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            3..1619
                     /gene="nikA"
                     /locus_tag="VBC28_001427"
     CDS             3..1619
                     /gene="nikA"
                     /locus_tag="VBC28_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001253836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
                     /protein_id="HEO6815407.1"
                     /translation="MRRNILLSITCLLMVTLTACHSQDSKSHKLNSDKLTLAWGEDFG
                     DVNPHRYNPDQFVIQDMVYEGLVRYGDNGKIEPALAKSWSISQDGKTYTFKLRNAKYS
                     DGSNFNAANVKRNFDSIFSKSNRGNHNWFNLTNQLENYRALNQSTFEIKLKQAYSATL
                     YDLSMIRPIRFLSDSAFPKGDDTTKKNVKKPIGTGQWVVKSKKQNEYITFKRNENYWG
                     KKPKLKEVTVKVIPDAQTRALAFESGDVDLIYGNGIIGLDTFAQYTKDKKYVTAISQP
                     MSTRLLLLNAKESIFQDKKVRQAMNHAIDKVSIAKNTFRGTEKPADTIFSKSTSHSDA
                     KLNPYSYNVDKANQLLDQAGWKMGKDKVREKDGKTLTLRLPYIATKATDKDLVTYFQG
                     EWRKIGINVSLIAMEEDDYWANAKKGNFDMMLTYSWGAPWDPHAWMSALTAKADHGHP
                     ENIALENLATKTEMDRLIKSALVDPKEENVDRDYKKVLELLHDEAVYIPLTYQSVISV
                     YRKGDFKTMRFAPEENSFPLRYIEKNNVSK"
     gene            1606..2550
                     /locus_tag="VBC28_001428"
     CDS             1606..2550
                     /locus_tag="VBC28_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6815408.1"
                     /translation="MYLSSSIIKKILSAFLALFFISLLTFILIKLSTVNPAENYLRLS
                     KISVSPEALKEAEHYLGLDKPLWKQYWLWFQKALTGDFGYSYVLRLPVLDLVLQRFLA
                     TLFLGTSAFLLIVTISTPLGVWAGLHESARSDHLIRFLSFSSVSMPNFWVAYLLMLLF
                     SAKLNLLPVSGGNDLQSLILPSITLSFSTVGQYIALIRKAISQENRSLNVENARLRGV
                     KERYIVTHHLLRNALPAIMTALSLTWVYLLTGSIIVEEIFSWNGIGRLFVTSLRTSDL
                     PVIQACMLIFGTLFLANNFMTQCFMNWVDPRLRKSREK"
     gene            2550..3356
                     /locus_tag="VBC28_001429"
     CDS             2550..3356
                     /locus_tag="VBC28_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6815409.1"
                     /translation="MSKRIYFSSSLLVLLVISAIFAPILSSFDPQYVDLSQKLLAPNN
                     VHLLGTDQLGRDVLSRLLYGARYSLFLAIIISLLELTIGMFVGLIVGWYQGKLENLFL
                     WIANIILAFPSFLLSLATVGILGHGLGNLIFAIVFVEWVYYAKLMTNLVKSAKKEPYV
                     INAQIMGLSVWHILRKHIFPFVYQPILVMVLMNIGNIILMISGFSFLGIGVQPNVTEW
                     GMMLHDARGYFRTATWMMLSPGIAIFLTVFSFNTLGDAIDKKDWKRQWNS"
     gene            3344..4132
                     /locus_tag="VBC28_001430"
     CDS             3344..4132
                     /locus_tag="VBC28_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6815410.1"
                     /translation="MEQLEIRKLSLQIGEVPVLRDFSCKIDMGESLTIIGESGSGKTL
                     LAKLLVGHIPQGMTVRGNIFFKGVDLGKLTVKQWQKLRGRDIAYLVQNPMSMFNPFQK
                     IEAHILETILSHEKCSKRVALSKALEWMKRLNLDDAISLLKKYPFELSGGMLQRIMLA
                     TILSLDPQVIILDEPTSAVDCHNCSTISAILQELQNNGKTLITVTHDYQLARDLGGQL
                     LVISEGEVVEQGQTQAILSNPQHNYTKALTVQMEYEGDILNVGL"
     gene            4119..4799
                     /locus_tag="VBC28_001431"
     CDS             4119..4799
                     /locus_tag="VBC28_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6815411.1"
                     /translation="MLVCNHVGKTFGRQEVLKDCHFHLKRGEIIGIMGKSGSGKSSLA
                     RLIIGLDSPTCGSIYFQGKIYTPKDGKAQIILVFQDALSSVNPYFSIEEILNEAFYGK
                     KTTFELCQILEAVGLDGTYLKYKARQLSGGQLQRVCIARALLLKPKIIIFDESLSGLD
                     PVTQIKMLRLLQKIKRRYELSFIMISHDPKICQAICNRVFLIKNGYLVEDNEFLKRAC
                     STNCLTNL"
     gene            4920..5648
                     /locus_tag="VBC28_001432"
     CDS             4920..5648
                     /locus_tag="VBC28_001432"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEO6815412.1"
                     /translation="MEPKYQRILIKLSGEALAGDKGVGIDIPTVQSIAKEIAEVHNSG
                     VQIALVIGGGNLWRGEPAAEAGMDRVQADYTGMLGTVMNALVMADSLQQYGVDTRVQT
                     AIPMQTVAEPYVRGRALRHLEKNRIVVFGAGIGSPYFSTDTTAALRAAEIEAEAILMA
                     KNGVDGVYNADPKKDANAVKFDELTHVEVIKRGLKIMDATASTISMDNDIDLVVFNMN
                     ETGNIKRVVLGEQIGTTVSNKASE"
     gene            5664..6221
                     /gene="frr"
                     /locus_tag="VBC28_001433"
     CDS             5664..6221
                     /gene="frr"
                     /locus_tag="VBC28_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEO6815413.1"
                     /translation="MTKEIVTKAQERFEQSHQSLSREFAGIRAGRANASLLDRIQVEY
                     YGAPTPLNQLASITVPEARVLLISPFDKSSIKDIERAINESDLGINPANDGSVIRLVI
                     PALTEETRRDLAKEVKKVGENAKIAIRNIRRDAMDEAKKQEKNKEITEDDLKSLEKDI
                     QKATDDAVKHIDEMTANKEKELLEV"
     gene            6339..7193
                     /gene="cvfB"
                     /locus_tag="VBC28_001434"
     CDS             6339..7193
                     /gene="cvfB"
                     /locus_tag="VBC28_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding virulence regulatory protein CvfB"
                     /protein_id="HEO6815414.1"
                     /translation="MNTLLATVITGLVTDENKDFYFIQKDGFTFALSKSEGEHHIGEM
                     VKGFAYTDMQQKARLTTKETFATRDHYGWGTVTEVRKDLGVFLDTGLPDKQVVVSLDV
                     LPELKELWPKKGDRLYVCLDVDKKDRLWALPADPEVFQRMATPAYNNMQNQNWPAIVY
                     RLKLSGTFVYLPENNMLGFIHPSERYSEPRLGQVLDARVIGFREVDRTLNLSLKPRSF
                     EMLENDAQMILTYLESNGGFMTLNDKSSPEEIKATFGISKGQFKKALGGLMKAKKIKQ
                     DQLGTELL"
     gene            7331..7840
                     /gene="msrA"
                     /locus_tag="VBC28_001435"
     CDS             7331..7840
                     /gene="msrA"
                     /locus_tag="VBC28_001435"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEO6815415.1"
                     /translation="MERAIFAGGCFWCMVQPFEELDGIESVLSGYTGGHVENPTYKEV
                     CSKTTGHTEAVEIIFNPEKISYADLVELYWAQTDPTDAFGQFEDRGDNYRPVIFYENE
                     EQRQIAQKSKDKLQASGRFDRPIVTSIEPADTFYPAEDYHQAFYRTNPARYALSSARR
                     HAFLEENWH"
     gene            7837..8052
                     /locus_tag="VBC28_001436"
     CDS             7837..8052
                     /locus_tag="VBC28_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HEO6815416.1"
                     /translation="MRKSFYSWLMTQRNPKSNEPVAILADYAFDETTFPKHSSDFETV
                     SRYLEDEASFSFNLTDFDDIWEDYLNH"
     gene            8209..9981
                     /locus_tag="VBC28_001437"
     CDS             8209..9981
                     /locus_tag="VBC28_001437"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HEO6815417.1"
                     /translation="MRYTNGNFEAFARPRKPEGVDKKSAYIVGSGLAGLAAAVFLIRD
                     GQMDGQRIHIFEELPLSGGSLDGVKRPDIGFVTRGGREMENHFECMWDMYRSIPSLEV
                     PDASYLDEFYWLDKDDPNSSNCRLIHKQGNRLESDGDFTLGTHSKELVKLVMETEESL
                     GAKTIEEVFSKEFFESNFWTYWATMFAFEKWHSAIEMRRYAMRFIHHIGGLPDFTSLK
                     FNKYNQYDSMVKPIISYLESHNVDVQFDSKVTNISVDFKNGQKLAKAIHLTVGGEAKT
                     IDLTPNDFVFVTNGSITESTNYGSHDTVAKPNTDLGGSWNLWENLAAQSDEFGHPKVF
                     YKDIPKESWFVSATATIKDPAIEPYIERLTHRDLHDGKVNTGGIVTVTDSNWMMSFAI
                     HRQPHFKEQKENETIVWIYGLYSNVEGNYIKKPIEECTGREITEEWLYHLGVPEMKIH
                     DLSDKQYVSTVPVYMPYITSYFMPRVKGDRPDVIPQGSVNLAFIGNFAESPSRDTVFT
                     TEYSIRTAMEAVYTFLNIERGVPEVFNSAFDIRVLLQSLYYLNDKKSVEDMDLPIPAL
                     MRKVGMKKIRGTYLEELLREAHLL"
     gene            10105..11091
                     /locus_tag="VBC28_001438"
     CDS             10105..11091
                     /locus_tag="VBC28_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001158936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEO6815418.1"
                     /translation="MQEYSIEITLQHPDDMMSLFGSNERHLKLIEENLDVIIHARTER
                     VQVLGDSEEAVETARLTIEALLVLVNRGMTVNTSDVVTALSMAQNGSIDKFVALYEEE
                     IIKDSYGKPIRVKTLGQKIYVDSVKNHDVVFGIGPAGTGKTFLAVTLAVTALKRGQVK
                     RIILTRPAVEAGESLGFLPGDLKEKVDPYLRPVYDALYQILGKEQTSRLMEREIIEIA
                     PLAYMRGRTLDDAFVILDEAQNTTIMQMKMFLTRLGFNSKMIVNGDVSQIDLPKNVKS
                     GLIDAVEKLRNIKKIDFIHLSAKDVVRHPVVAEIINAYSDSESSHKLQSDKD"
     gene            complement(11182..11985)
                     /locus_tag="VBC28_001439"
     CDS             complement(11182..11985)
                     /locus_tag="VBC28_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000349560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4037 domain-containing protein"
                     /protein_id="HEO6815419.1"
                     /translation="MDHFTKLWQDFSKLPNVVAIALGGSRSGDSFDQSSDYDLYVYCA
                     ATPDITSRKRILNKHCHYIELNNHYWELEDNGTLNDGTDIDILYRNIDNFLSDLEDVV
                     EHHNSRIGYTTCFWHNLINCQILYDPENQLQSLKERFEVSYPSQLQKQIIIQNRNLLT
                     GKLPSYDKQIIKALKRQDFVSTHHRTTAFLDSYFDIIFALNKLTHSGEKRMISYAKKN
                     ATLLPKHFEENIIKLCHHNSNEHTVKETLNDIIMHLDVMLKENFQHFIG"
     gene            12177..12653
                     /locus_tag="VBC28_001440"
     CDS             12177..12653
                     /locus_tag="VBC28_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEO6815420.1"
                     /translation="MKQDYISYIRSKVGHETIFLTYSGGILTDGKGRVLLQLRADKNS
                     WGIIGGCMELGESSVDTLKREFFEETGLRVEPIRLLNVYTNFQDSYPNGDKAQTVGFI
                     YEVSCPKPVNIEGFHNEETLQLDYFSKEDVKNITIVNEQHQLILDEYFSQTFQMGR"
BASE COUNT         4221 a         1923 c         2587 g         4140 t
ORIGIN      
        1 aaatgagacg taacatatta ttgagcatta cttgtttatt gatggtgact ctgactgcat
       61 gccattcaca ggatagcaaa agtcataaac taaacagtga taaactcacg cttgcttggg
      121 gagaagactt tggtgatgtt aatccgcatc gctataaccc tgatcagttt gttatccagg
      181 atatggttta tgaggggttg gtacgatatg gtgacaatgg gaaaattgaa ccagctttag
      241 cgaaaagctg gagcattagt caagatggaa aaacctacac ttttaaattg agaaatgcta
      301 aatattctga tggcagtaac tttaatgctg cgaatgtcaa acgtaatttt gatagtattt
      361 tttcgaagtc taatagaggt aatcataatt ggtttaattt aacgaatcaa ttggaaaatt
      421 atcgtgcctt gaatcaaagt acttttgaaa ttaaactgaa acaagcttat agtgctactt
      481 tatatgatct ctctatgatt cgaccaattc gctttttatc tgatagcgct ttccctaaag
      541 gtgatgatac aactaagaag aatgtaaaaa aaccaattgg aacgggtcaa tgggttgtga
      601 aaagtaaaaa gcaaaatgaa tatattacct tcaaacgtaa tgagaattac tggggaaaga
      661 aaccaaaatt aaaagaagta accgtaaaag ttatacccga tgctcagacg cgtgctttag
      721 cattcgaatc aggtgacgtt gatttgatct atgggaatgg cattattggt ttagacactt
      781 ttgcccaata tacgaaggac aagaaatatg tcacagctat ttctcaacct atgtcaacaa
      841 gacttttgtt gctaaatgct aaggaaagta ttttccaaga taaaaaagtt cgtcaggcaa
      901 tgaatcatgc tattgacaag gtttctattg ctaagaacac ctttagagga acagaaaagc
      961 cagcagacac tattttttca aaatcaacat ctcactcaga tgctaagtta aatccttata
     1021 gctataatgt tgataaagca aatcagctat tggatcaagc tggttggaag atgggaaaag
     1081 ataaggttcg tgaaaaagat ggtaaaactc taaccttacg tcttccttat atcgccacta
     1141 aggcaactga taaagacctg gtaacttatt tccaaggaga atggcgtaaa atcggtatta
     1201 atgtttctct tattgctatg gaagaagatg attattgggc taatgctaaa aagggtaatt
     1261 ttgatatgat gttaacctat tcttggggag caccatggga ccctcatgct tggatgtcgg
     1321 cattaactgc caaggcagat catggacatc cagaaaatat agctttagaa aatctagcaa
     1381 ctaaaactga aatggataga cttattaagt cagctctagt agatcctaag gaagaaaatg
     1441 ttgatagaga ctataagaag gttcttgaat tattgcatga tgaggctgta tatatcccat
     1501 taacttatca atcggtgatt tctgtttata ggaaaggtga ttttaaaacc atgcgttttg
     1561 cgccagaaga aaattcattc ccattacgct atatagaaaa aaacaatgta tctaagtagt
     1621 tcgatcatca aaaaaatatt gtcagctttt ttggcattgt ttttcatatc acttctaaca
     1681 tttattctta tcaaactatc aacagttaat ccagcagaaa attaccttcg tttatcaaaa
     1741 ataagtgtta gtccagaagc gctaaaagaa gcagagcact atttaggttt agataagcca
     1801 ttatggaagc agtactggct gtggttccaa aaagcattaa caggagattt tggatattct
     1861 tatgtgctca ggttgccagt attagatttg gttttacaac ggtttttggc tactttattt
     1921 ttaggcacga gtgccttctt gctaattgtt accatttcta ccccgttagg tgtgtgggct
     1981 ggcttacatg aatctgctcg gagtgatcac ttgattcgat ttttgagttt ttctagtgtg
     2041 tctatgccaa atttttgggt tgcttacttg ttgatgctgc tattttcggc aaaattaaat
     2101 cttctgcctg tttcaggagg taacgattta caaagtttaa ttttgccaag tattacctta
     2161 agtttttcta ccgtaggtca atacatagcc cttattcgaa aagctattag tcaagaaaat
     2221 agaagtttaa atgtggaaaa tgcccgatta agaggagtaa aagaacgtta tattgtcaca
     2281 catcacctcc ttagaaatgc cttacctgcg ataatgacag cacttagctt gacttgggtt
     2341 tacttattaa caggatcaat tattgttgaa gaaatttttt catggaatgg aataggacgt
     2401 ttatttgtga ccagtctaag aacttcagat cttccagtaa tacaagcttg tatgctaatt
     2461 tttggaacct tatttttagc taataatttt atgacacagt gttttatgaa ttgggttgat
     2521 cctcggttac gaaagtcaag ggagaaataa tatcaaaaag aatttatttt tcgagtagct
     2581 tactggtatt gttagtgatt agtgccattt ttgcccctat tctatctagc tttgatccac
     2641 aatacgtaga tttatcacaa aaattattgg ctcccaataa tgttcatttg ttagggactg
     2701 accaattggg tagagatgta ttatctcgct tgctttatgg tgctagatat tcactgtttt
     2761 tagctattat tattagctta ttggaattaa cgattggtat gtttgtgggt cttattgttg
     2821 gttggtatca aggaaagtta gaaaacctct ttttatggat agctaatatt attttagctt
     2881 ttccgagctt tttgctctct cttgcaaccg tggggatctt aggtcatggt ctagggaatt
     2941 taatttttgc aattgtcttt gtggaatggg tttactatgc taaattaatg accaatttag
     3001 ttaagagtgc aaaaaaagag ccttatgtga taaatgcgca aattatggga ctttcagtct
     3061 ggcatatttt aagaaaacat atttttcctt ttgtgtatca gccaatcctc gttatggtcc
     3121 ttatgaatat aggaaatatt attttaatga tttctggctt ttctttctta gggattggtg
     3181 tgcaacctaa tgtcacagaa tggggaatga tgttgcacga tgctagagga tattttagga
     3241 cagctacttg gatgatgtta tctcctggaa ttgcgatttt tttaacagta ttttccttta
     3301 atactttagg cgatgccata gataaaaaag attggaaacg acaatggaac agttagaaat
     3361 aagaaaattg tcattacaga taggagaggt ccctgtacta agagatttta gttgtaaaat
     3421 agatatggga gaatcgctaa ctattattgg tgagagtggt tcagggaaaa ccctcttggc
     3481 aaaattattg gttggtcaca ttccacaagg tatgacagtt agaggaaaca tattttttaa
     3541 gggagttgat ttaggtaaac taactgtaaa gcagtggcag aaattaaggg gacgagatat
     3601 tgcctattta gtgcaaaatc ccatgtctat gtttaatcct tttcaaaaaa ttgaagcgca
     3661 tattttggaa acaatcctta gtcatgagaa atgttcaaag agagtagcct tatctaaagc
     3721 gttagaatgg atgaaacgtt taaatttaga tgatgcgata tccctgttaa aaaaataccc
     3781 ttttgagctt agtggaggaa tgctacaaag aattatgtta gccactatat tatctcttga
     3841 tcctcaggta atcatattag atgagccgac ctcagcggtt gattgtcata attgttctac
     3901 tatatcagct attttgcagg aattgcaaaa taatggaaaa accttgatta ctgtaacgca
     3961 tgattatcag ttagctagag acctgggggg acaattatta gttataagtg agggagaagt
     4021 tgttgaacag ggacaaaccc aagctatttt aagcaatcct caacataact atacgaaagc
     4081 actgacagtg caaatggagt atgaaggaga tattcttaat gttggtttgt aatcatgtcg
     4141 gaaaaacatt tggacgacaa gaggttttaa aggactgtca ttttcatctt aaaagaggag
     4201 aaataattgg tataatgggt aaaagtggca gtggtaaaag tagccttgcc cgactcatta
     4261 taggactaga tagtcctact tgtggttcga tatattttca aggaaaaatt tacacaccta
     4321 aagatggtaa ggcgcagatt atccttgttt ttcaagatgc gcttagttcg gtcaatccat
     4381 attttagtat tgaggaaatt ttgaatgaag ctttttatgg aaaaaaaaca acttttgaat
     4441 tatgtcaaat attagaagcg gtgggtttag atggaactta tctaaaatat aaagctagac
     4501 aacttagtgg tggtcagcta cagagagttt gtatcgctag agctttactc ttaaagccta
     4561 aaattattat ttttgatgaa tccttaagtg gattagaccc agtaactcaa attaaaatgc
     4621 tacgtttatt acaaaaaata aaacgccgtt atgaattaag ttttataatg atttctcatg
     4681 accctaaaat ttgtcaagct atctgtaacc gggtctttct gataaaaaat ggttatttag
     4741 tagaagataa tgaattcttg aagagagcgt gttctactaa ctgtttgacc aatctctgaa
     4801 ttattatgtt tttcttattt taggagttga taaagaaatc aacttactag tgtcattgct
     4861 ctattaaaat tttgcctttt atggtaaaat aataaagaca aaataaggag aacatagtag
     4921 tggaacctaa atatcaacgt atattaatta agttatctgg tgaagcattg gcgggagata
     4981 agggtgtcgg tattgacatt cctaccgttc aatctattgc aaaagaaatt gccgaagtac
     5041 acaattcagg tgttcaaata gcgcttgtca ttggtggagg taatctttgg cgtggagaac
     5101 ctgcagcaga agcagggatg gatcgtgttc aagctgatta tacagggatg ttagggacag
     5161 taatgaatgc tcttgtaatg gctgatagcc ttcaacaata tggggtagat actagagttc
     5221 aaacagctat tccaatgcaa actgttgctg agccttacgt acgtggtcgt gctttacgtc
     5281 atctagagaa gaatcgtatt gttgtctttg gagcgggtat tggttcgcct tatttctcaa
     5341 ctgatacaac agcagcgctt cgtgctgctg agattgaggc agaagcaatt ttaatggcta
     5401 aaaatggagt agatggtgtt tataatgctg atccaaagaa agatgccaat gctgtcaaat
     5461 ttgatgaatt aactcacgtt gaagtaataa aacgagggtt aaaaatcatg gatgcaaccg
     5521 catcaactat ttcaatggat aatgatattg accttgttgt tttcaatatg aatgagactg
     5581 gtaatattaa gcgtgttgtt cttggagaac aaatcggaac aactgtttca aacaaagcat
     5641 ctgaatagga gtgtataaaa aatatgacta aggaaatcgt tacaaaagct caagagcgtt
     5701 ttgagcaatc acaccaaagt ttatcaagag aatttgcagg gattcgtgca ggacgtgcta
     5761 atgctagctt gcttgatcgt attcaagttg agtattatgg agcgccaacc ccccttaacc
     5821 aattagcttc tattactgta cctgaagctc gtgtcctttt aatttcacca tttgataaat
     5881 catcaatcaa agatattgag cgtgctatta acgaatcaga tttaggtatt aatcctgcaa
     5941 atgatggttc agttattcgt cttgttattc cagctttgac agaagaaaca cgtcgtgatt
     6001 tagctaaaga agtgaaaaaa gtcggagaaa atgctaagat tgctatccgt aatattcgcc
     6061 gtgatgctat ggatgaagct aagaagcaag aaaagaataa agaaattaca gaagatgact
     6121 tgaaatctct tgaaaaagat attcaaaagg ctactgatga tgctgtaaaa catattgatg
     6181 agatgactgc taataaagaa aaagaattgt tggaagttta atagtaacgt ttgctatctt
     6241 tattatgatt ggcaagtaat aaaatgatta tggaggtaac tgatagaatc aaaaccagat
     6301 ggatttggtt tgtcggttac ctttttaaaa ggagaaaaat gaatacatta ttagcgacag
     6361 tcataacagg cttagtaact gatgaaaaca aagattttta ctttatccaa aaagatggct
     6421 ttacctttgc attatcaaag tcagaaggag agcatcacat tggtgaaatg gttaaaggat
     6481 tcgcctatac agatatgcaa caaaaagcac gtcttacaac taaagaaaca tttgcgactc
     6541 gagatcatta tggttggggg acagttactg aggtacgtaa agacttggga gtatttcttg
     6601 atactggtct tccagataag caagtggttg tttctttgga tgttcttcct gaattgaaag
     6661 aactttggcc taaaaaaggt gatcgcctct acgtttgctt agatgttgat aaaaaagatc
     6721 gtctctgggc tctccctgca gatcctgaag tgtttcaacg aatggcaacc cctgcatata
     6781 acaatatgca aaatcaaaat tggccagcaa tcgtttatcg cttaaaattg tcaggaactt
     6841 ttgtttatct accagaaaat aatatgctcg gctttattca ccctagtgaa cgttacagtg
     6901 aacctcgttt agggcaagtg ctggatgcac gagttattgg cttcagagaa gttgatagaa
     6961 ccttaaattt atcattaaag cctcgttctt ttgaaatgtt agaaaatgac gcacaaatga
     7021 ttttaacgta tttagaaagt aacggaggat tcatgacttt aaatgataaa tcttctccag
     7081 aggaaattaa agctactttt ggaatttcca aaggtcagtt taaaaaagca cttggaggac
     7141 tcatgaaagc taaaaagatc aaacaagatc agctaggtac agaattatta taaacttttg
     7201 aacttttaaa gttttctcac gaggtcgttt tagttatagc tagaattaat ggctaattat
     7261 agaaaaaagc tgagaggaaa ttttggcttt ttttgttata atgtatctat taaaaagaat
     7321 ggagaacgat atggaacgtg cgatttttgc tggaggttgt ttttggtgta tggtacaacc
     7381 atttgaagaa ttagatggta ttgaatctgt tttatctgga tacactggag gacatgttga
     7441 gaatccaact tataaagaag tttgtagtaa aactactgga catacagaag ctgttgaaat
     7501 tatttttaat cctgaaaaaa tctcttatgc agatttagtt gagttatatt gggcacaaac
     7561 agatccaaca gacgcttttg gtcagtttga agatcgtggg gataattatc gtcctgtcat
     7621 cttttatgag aatgaagagc aacgtcagat tgctcaaaaa tcaaaagata agctacaagc
     7681 ttcgggtaga tttgatagac caattgtaac cagcattgag cctgctgata ctttttaccc
     7741 agcagaagac tatcatcaag cattctatcg gactaaccct gctcgttacg ccttgagtag
     7801 tgccagaaga catgcatttc tagaggagaa ctggcattga gaaaatcatt ttacagttgg
     7861 ttgatgacac aaagaaatcc aaaatcaaat gaaccagtag caatcctagc agattacgct
     7921 tttgatgaaa ccacttttcc aaaacatagt tcggattttg aaacagttag tcgctattta
     7981 gaagatgagg ccagtttttc ctttaattta acagattttg atgatatttg ggaagattat
     8041 ctcaatcatt aacaataata tagagattgt attttcaatc tcttttttag gttatagaaa
     8101 ttggacacat tgtctaagac tgtctgaaaa gcaggttttt taatggcaat cgttttcaca
     8161 aaggagtaga atatagtcat ctaaaatata gccctaggga ggttgtttat gagatataca
     8221 aatggaaatt ttgaagcctt tgcaagacct cgaaaacctg aaggtgtgga taaaaaatcc
     8281 gcttatattg ttggttctgg tttagcagga ttagctgccg ctgtcttttt aatacgtgac
     8341 ggtcaaatgg atggtcaacg tattcatatt tttgaagaac tacctctttc tggaggatca
     8401 cttgacggtg tcaaacgacc tgatatcggt tttgtaacgc gtggtggtcg tgaaatggaa
     8461 aatcacttcg aatgtatgtg ggatatgtac cgttccatcc cctctctcga agttccagat
     8521 gcttcttatc tagatgaatt ttattggctt gacaaggatg atcccaattc atctaactgt
     8581 cgcctcattc ataaacaggg gaatcgctta gaatctgatg gtgattttac actcggaaca
     8641 cattccaaag agttagttaa gctagtcatg gagactgaag agtctttagg tgctaagacg
     8701 attgaagaag ttttttcaaa agaatttttt gaaagtaatt tttggactta ttgggctact
     8761 atgtttgcct ttgagaaatg gcattcagcg attgaaatgc gtcgatatgc tatgcgcttt
     8821 atccatcata ttggtggtct gcctgatttc acttcattaa aatttaataa atataatcaa
     8881 tatgattcta tggtgaaacc aatcatcagt tatttagagt ctcataatgt agatgttcaa
     8941 tttgatagca aggtaactaa tatctccgta gactttaaga acggtcagaa gcttgcaaaa
     9001 gctattcatc tcacagttgg tggtgaagca aaaacaattg atcttactcc aaatgatttt
     9061 gtttttgtaa cgaatggttc tatcactgaa agtacgaact atggtagtca tgatactgta
     9121 gctaaaccta ataccgattt gggaggttct tggaatcttt gggaaaactt agctgctcaa
     9181 tctgatgaat ttggacatcc taaagtcttc tacaaagata ttccaaaaga gtcatggttt
     9241 gtttctgcta ctgcaacaat aaaagatcca gctattgaac cttatataga gcgtttaaca
     9301 catcgtgacc tccatgatgg taaagtaaat accggaggca ttgttacagt tacagattct
     9361 aattggatga tgagttttgc tatccatcgc caaccgcatt tcaaagagca aaaggaaaat
     9421 gaaacaattg tttggattta tggactctat tcaaatgtgg aaggtaatta tattaaaaaa
     9481 ccaattgaag aatgtactgg tcgagaaatc acagaagagt ggttatatca cttaggtgtt
     9541 cctgaaatga agattcatga tttatctgat aaacaatatg taagtacagt tcctgtttat
     9601 atgccttaca ttactagtta tttcatgcct cgagttaaag gtgaccgacc agatgttatc
     9661 ccacaaggat ctgttaacct agccttcatt ggtaattttg cagaatctcc atccagagat
     9721 acagtattta caacggaata ttctattaga actgctatgg aagctgttta tactttctta
     9781 aatattgaac gtggtgttcc agaagtcttt aattcagcat ttgatattcg tgtcttgttg
     9841 caatctttat actatctcaa tgataagaaa tctgttgaag atatggattt accaatacct
     9901 gccttaatgc gtaaagtagg catgaaaaaa attagaggaa cttatttaga agaattactt
     9961 cgagaagctc atcttttata atgtaaaata aaatcgagaa aacttgtttt tctcgatttt
    10021 ttagttttac aacgactaaa atcaccaaag aaaatgaaat tttgttataa tgtagtcact
    10081 tactgtacga aaaggaagaa taatttgcaa gaatactcta tcgaaattac tttacaacat
    10141 ccagatgata tgatgtcact ctttggcagc aatgagcgcc atttaaaatt aatcgaagaa
    10201 aatttagatg ttatcattca tgctagaact gaacgtgttc aggtattagg tgactctgaa
    10261 gaagcagtag aaaccgctcg gttaaccatt gaagcattat tagtgcttgt taaccgcggg
    10321 atgactgtga atacttcaga tgttgtaaca gccttatcca tggcacaaaa tggttcaatt
    10381 gataaattcg ttgcccttta tgaggaagaa attattaagg atagctatgg taagcctatc
    10441 cgtgttaaga ctttagggca aaaaatatac gttgatagcg ttaaaaatca tgatgttgtt
    10501 tttggtattg ggcctgctgg aacgggaaaa acttttttag cagtcactct agcagtaacg
    10561 gcactaaagc gtggtcaagt taaacgaata atcttaacga gaccagcggt tgaagctggt
    10621 gaaagccttg gtttcttgcc tggagatctc aaagagaaag ttgatcctta tttgagacca
    10681 gtttatgatg ccctatatca aattttaggt aaagagcaaa caagtcgctt aatggagcgt
    10741 gaaatcatcg aaattgctcc attggcttac atgcgtggtc gtactttaga tgatgcattt
    10801 gtcatattag atgaagcgca aaacaccact ataatgcaaa tgaaaatgtt cctaacacgt
    10861 cttggcttca attctaaaat gattgtaaat ggcgatgtta gccaaattga cttaccaaag
    10921 aatgttaaat ctggtttgat tgatgctgtt gagaaattac gaaatatcaa aaagattgac
    10981 tttattcatt tatcagcaaa ggatgttgta agacaccctg tcgttgctga aataataaat
    11041 gcttattcag attcagaaag tagtcacaag ctacaatctg ataaggactg atgtttgcca
    11101 ctaggagacg ttgttagaga gtgtgtcaat atgtaaaaaa cgcctaatgc tttaggcgtt
    11161 tttatgatgg tgtacttcaa cttaacctat aaagtgttga aaattttctt taagcatgac
    11221 gtcgagatgc attattatat cgtttaatgt ttctttaaca gtgtgttcgt tggagttgtg
    11281 atgacatagt ttaatgatat tttcttcgaa atgtttagga agcaatgtag cattcttctt
    11341 agcataggaa atcattcttt tttcgccaga atgtgtcaac ttattaagtg caaaaataat
    11401 atcaaagtag gaatctaaga aagcagtagt tctatggtga gtactaacaa agtcttggcg
    11461 tttaagggct tttataattt gtttatcgta agagggaagc ttgccagtta ataagttacg
    11521 attttgaatg ataatttgtt tttgtaactg actgggataa gaaacttcga atctctcttt
    11581 gagtgattgt aattgatttt caggatcata gagtatttgg caattgatga ggttatgcca
    11641 aaaacaagta gtgtatccaa ttcgagaatt gtggtgttca acgacatctt ctaagtctga
    11701 taaaaagtta tctatgttac gatagagaat atcaatatca gttccgtcgt ttaaagtacc
    11761 attatcttca agctcccagt aatgattgtt aagttcaatg taatgacagt gcttgttaag
    11821 gatacgttta cgactagtga tatcaggagt tgctgcacaa taaacataca aatcataatc
    11881 ggaagattgg tcaaaactgt caccagagcg tgagcctccg agtgctatag caacaacatt
    11941 tggaagttta gagaaatctt gccagagttt tgtgaaatgg tccataaatg ctctcctaaa
    12001 aatgtaaaat attgatgtgt ctccattcta cagggctatc tctttttagt caataattaa
    12061 tagtaagtta ataaaagagg taacggttct aatattcaag aagtgtatat gagtgatttg
    12121 aaaaggagat attttatgct acaatacgta catattagtt taaacaaggt gatagaatga
    12181 aacaagatta tatttcctac attaggtcaa aagttggaca tgagactatc tttttaacat
    12241 attctggtgg tattttgaca gatggtaagg gacgtgtctt actccaatta agagctgata
    12301 agaattcttg gggaattatt ggtggatgta tggagttagg tgagtcatca gtagatacat
    12361 tgaaaagaga atttttcgaa gaaactggat tgagagtgga accaatcaga ctgttaaatg
    12421 tttatactaa ctttcaagac tcttatccca atggcgataa agcacaaacc gtcggtttta
    12481 tttatgaagt aagctgtcca aaaccagtta atatagaagg ttttcataat gaggaaacat
    12541 tgcaattgga ctatttttct aaagaagacg tcaaaaatat tacgatcgta aatgaacaac
    12601 accaattaat tttagatgaa tatttttcac aaacattcca aatggggcgt taaaaggatg
    12661 ggaaatacta ggtgtgactt taaagaactt ctaactttta ttgatatgtt gttaggcatt
    12721 cttgtagaaa caaatgttaa gaaaataagg agttctcatt tgtatatttt ataattatta
    12781 aaaagaaaaa cagtagcttg caataattta gtgactgttt ttttagaaag cataagaagt
    12841 acagaaaaag aaaattatgg aatatttgat g
//
LOCUS       DATRMA010000053        12585 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.053, whole genome shotgun sequence.
ACCESSION   DATRMA010000053 DATRMA010000000
VERSION     DATRMA010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12585
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.053"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            156..548
                     /gene="merR"
                     /locus_tag="VBC28_001441"
     CDS             156..548
                     /gene="merR"
                     /locus_tag="VBC28_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020220203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hg(II)-responsive transcriptional regulator"
                     /protein_id="HEO6815421.1"
                     /translation="MIYRISEFADKCGVNKETIRYYERKNLLQEPHRTEAGYRIYSYD
                     DVKRVGFIKRIQELGFSLSEIYKLLGVVDKDEVRCQDMFEFVSKKQKEVQKQIEDLKR
                     IETMLDDLKQRCPDEKKLHSCPIIETLT"
     gene            577..2217
                     /gene="merA"
                     /locus_tag="VBC28_001442"
     CDS             577..2217
                     /gene="merA"
                     /locus_tag="VBC28_001442"
                     /EC_number="1.16.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001031939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mercury(II) reductase"
                     /protein_id="HEO6815422.1"
                     /translation="MNKFKVNISGMTCTGCEKHVESALEKIGAKNIESSYRRGEAVFE
                     LPDDIEVESAIKAIDEANYQAGEIEEVSSLENVALINEDNYDLLIIGSGAAAFSSAIK
                     AIEYGAKVGMIERGTVGGTCVNIGCVPSKTLLRAGEINHLSKDNPFIGLQTSAGEVDL
                     ASLITQKDKLVSELRNQKYMDLIDEYNFDLIKGEAKFVDASTVEVNGTKLSAKRFLIA
                     TGASPSLPQISGLEKMDYLTSTTLLELKKIPKRLTVIGSGYIGMELGQLFHHLGSEIT
                     LMQRSERLLKEYDPEISESVEKALIEQGINLVKGATFERVEQSGEIKRVYVTVNGSRE
                     VIESDQLLVATGRKPNTDSLNLSAAGVETGKNNEILINDFGQTSNEKIYAAGDVTLGP
                     QFVYVAAYEGGIITDNAIGGLNKKIDLSVVPAVTFTNPTVATVGLTEEQAKEKGYDVK
                     TSVLPLGAVPRAIVNRETTGVFKLVADAETLKVLGVHIVSENAGDVIYAASLAVKFGL
                     TIEDLTETLAPYLTMAEGLKLVALTFDKDISKLSCCAG"
     gene            2377..3630
                     /locus_tag="VBC28_001443"
     CDS             2377..3630
                     /locus_tag="VBC28_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEO6815423.1"
                     /translation="MSDLLSLPDIKTIEPPQENETDMMFKVEAVGPPERCPECGFDKL
                     YKHSSRNQLIMDLPIRLKRVGLHLNRRRYKCRECGSTISVDEKRSMTKRLLKSIQEQS
                     MSKTFVEVAESVGVDEKTIRNVFKDYVALKEREYQFETPKWLGIDEIHIIRRPRLVLT
                     NIERRTIYDIKPNRNKETVIQRLSEISDRTYIEYVTMDMWKPYKDAVNTILPQAKVVV
                     DKFHVVRMANQALDNVRKSLKAHMSQKERRTLMRERFILLKRKHDLNERESFLLDTWL
                     GNLPALKEAYELKEEFYWIWDTPDPDEGHLRYSQWRHRCMSSNSKDAYKDLVRAVDNW
                     HVEIFNYFDKRLTNAYTESINSIIRQVERMGRGYSFDALRAKILFNEKLHKKRKPRFN
                     SSAFNKAMLYDTFNWYEVNDHDITD"
     gene            3617..6115
                     /locus_tag="VBC28_001444"
                     /pseudo
     CDS             3617..6115
                     /locus_tag="VBC28_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771388.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            6175..6471
                     /locus_tag="VBC28_001445"
     CDS             6175..6471
                     /locus_tag="VBC28_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6815424.1"
                     /translation="MKQETLVLLPNFIGDAIYEGFEAGPAQLNGKKGSYTRHILFNEK
                     HGKFYVYTPASSRVIGNGKMVTVEKPLLFIDNTINGRNIEPAKNVWAEKLIEVK"
     gene            6493..6954
                     /locus_tag="VBC28_001446"
     CDS             6493..6954
                     /locus_tag="VBC28_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001062569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6815425.1"
                     /translation="MAKIGLNFGEKLQIADKEYRTFNDGLDVNVLLGDLTWRRYEEPV
                     QFTEDDTTQPPDRFGRYPQKPTGEVKWNDIAVFSTAQNETIFVSITDMPSYAIEDLGL
                     KLGDPIELSDVVVTWSNVSGGMFKIFASSLKKKDVANNGNASKNEASKVNK"
     gene            6974..8692
                     /locus_tag="VBC28_001447"
     CDS             6974..8692
                     /locus_tag="VBC28_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HEO6815426.1"
                     /translation="MARLIPQYRGIKVKPYMRYMSYYLFSFLFILFLTPVGVYSYYYL
                     DLLKMMDKMSMISVGTGLFLAFFVSWYLTWFLQEANPLFNKLDRLKRMSKFLYENGYV
                     YEKRKKSNKKTKTKYRFPKVYVKQGKYDLSVSFEMAGGKFQKKFKDIGGELEDTFFMD
                     FMEKTDDPRFKIYKLAYSAFLSRITVKDVIWDKDKGIKLMDGYYWDFINDPHLLVAGG
                     TGGGKTVLLRSILRCLAEIGVCDICDPKRADFVTMSDLSAFEGRIAFEKADIIEKFEN
                     AVTIMFARYDFVRNEMKRLGHKDMKKFYDYGLEPYFFVCDEYNALMSSLSYQEREIVD
                     NAFTQYILLGRQVGCNAIIAMQKPSADDLPTKIRSNMMHHISVGRLDDGGYVMMFGDE
                     NRNKEFRFIKYLAGRRVYGRGYSAVFGEVAREFYSPLLPKNFSFYDAFEKINRHENPF
                     DPTENQEVSKAILKDESLREFVEKTSENELLKGSVGFDFDDEMEEMENMSIGDLAKKT
                     GFSFGKIKNLIDMIEKDNYMTFSREDEKVVLNDSDAVVLESLLTQKDEFEGSWKELLL
                     NYFKGE"
     gene            8980..10212
                     /locus_tag="VBC28_001448"
     CDS             8980..10212
                     /locus_tag="VBC28_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEO6815427.1"
                     /translation="MDAIYLKKFRKKTGLKQKEFALSSGLTLKSLRNYEQGKRKLTLE
                     KYQEIKSHFGYLVENDSSRLQVMIDYVRITLKDVRDLEFFCRNFLHCAFKEFQPFESK
                     LMNYNHLWKRGDIWIFDFADKHETGNFQITVQLSGRGCRQLELLMETEKFTWHDWLSY
                     LRNSYRDDMNVTRFDIAIDELYLGKDRENEQFHLSDMISKYYRHELDFESLRTWNYIG
                     GGSLNFSDMEEIEQNRQGISLYFGSRQSEMYFNFYEKRYEIAKQEGITVEEALEIFEL
                     WNRYEIRLSQSKANAAVDEFISGVSIGEISRGLIVSKIDVYDGKNEYGSFQADRKWQL
                     MFGGVEPLKFVTKPEAYSIERTLRWLSDSVSPSLAMIREYDMIVDGDYLQTILNSGEV
                     NERGEKILDSIKASLGIL"
     gene            10228..10605
                     /locus_tag="VBC28_001449"
     CDS             10228..10605
                     /locus_tag="VBC28_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000287184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6815428.1"
                     /translation="MYSNREFAYFVNRRNNLDKMIDLLVFMIPDREFYYPEINQGELS
                     DYQKDIFDLIQFGYCGVYEVKKEYEDKLQQLATLKRKLLKFGLLMQPLEKQKEIVIRL
                     AGKYRLEKRILMRREMFRDEEVD"
     gene            10589..10804
                     /locus_tag="VBC28_001450"
     CDS             10589..10804
                     /locus_tag="VBC28_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001897988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO6815429.1"
                     /translation="MRKLTNFGENLRLTRKTKSLTQTELASKLNISQKSYSNWETGKS
                     EPNLENVVKLAKLFKTTTDFLLGVTDD"
     gene            10797..11150
                     /locus_tag="VBC28_001451"
     CDS             10797..11150
                     /locus_tag="VBC28_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO6815430.1"
                     /translation="MIENFALNLIRLRKQKGLSQKELAKKIGMLPQTISNIENQKGYP
                     TFSNLDKIARYFNVSATELFGTPQQIELERVMFQTDEYSRKAEKILSAVSVFENFVKK
                     YDYQKIIEFGLSLKE"
     gene            11282..11554
                     /locus_tag="VBC28_001452"
     CDS             11282..11554
                     /locus_tag="VBC28_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6815431.1"
                     /translation="MSNTRSEADKKLLVVTQELSELLTSHQYEQSWEKAGELNSLLKR
                     REELTLPGYMVDMIQQHLKSYYYQNNMINKAHKSMSAIGHKLQEFH"
     gene            11606..12088
                     /locus_tag="VBC28_001453"
     CDS             11606..12088
                     /locus_tag="VBC28_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000383283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HEO6815432.1"
                     /translation="MDVSAIVRDIKTGRATLVCFPVEMNELKLALGLREEDDLEYIIA
                     DSDCQLLKEHDSIEMINQFVELVENVDSELVKAVHQVIGYTASDFVDYDFNFGDCCLL
                     SDVTTRRELGEYYFDELGVQGVGKEALEMYFDHEAYGRDIDLESQGGFSDYGYVEISG
                     "
     gene            12096..12569
                     /locus_tag="VBC28_001454"
     CDS             12096..12569
                     /locus_tag="VBC28_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6815433.1"
                     /translation="MIAIIRTREKGRSQFLCELDIMQFTENQVRNRMIEKGIKDDAFF
                     ICGFSDWNVDRIMSLTEVYLLKRCIEGLYDGDDYIVQYLLKKGLSVHSIVTKYYIFLS
                     NNENKVMKYILRKVEFDSLIDFWQRSVTWVNALNAYIEEGIVLNTSKGFYVLKTE"
BASE COUNT         4449 a         1703 c         2588 g         3845 t
ORIGIN      
        1 atcggatttt ctggtgggat gttaggataa tcaatagtat tatataaaag tatcaatcac
       61 atacgagtga tactttggga aaaaaataaa atagtccaat ttacttgacc gtgtactatg
      121 gtacatggtt tataatattc agggggtgaa ataagatgat ttatcgcatt agtgagtttg
      181 cagataaatg tggagttaat aaagaaacga tcagatatta cgagcgaaaa aatttattac
      241 aagaacctca ccgaacggaa gctggttatc ggatatattc atatgatgac gttaagcgtg
      301 ttgggtttat taaacgaata caggaacttg gtttctcttt aagcgagatt tataaattac
      361 ttggtgttgt agataaagat gaagttcgtt gtcaagatat gttcgaattt gtttctaaaa
      421 aacaaaagga agtgcaaaaa caaatagagg atttaaaacg aattgaaact atgttagacg
      481 acttaaaaca acgatgtcca gatgaaaaga aattacattc gtgtccaata atagaaacat
      541 taacatgaga gattaattaa cgaaaggagc ttttttatga ataaatttaa ggtaaacatt
      601 tcaggaatga cttgtacggg ttgtgaaaaa cacgtagaat cagcacttga aaagataggt
      661 gctaaaaata ttgagtctag ttatcgtcgt ggtgaagcag tatttgaact gcccgatgat
      721 attgaggttg aaagtgcaat aaaggcgatt gatgaagcaa attaccaagc cggagaaatt
      781 gaagaagtat catcactaga aaacgtggcg ttaattaatg aagacaatta tgaccttctt
      841 attattggtt ctggtgctgc tgccttttct tcggcaatta aagctataga atacggtgca
      901 aaagttggaa tgattgagcg tggaacggtt gggggaacct gtgtgaatat tggctgtgtt
      961 ccgtcaaaaa ctcttcttag ggcaggggaa atcaatcatt tatcaaaaga caatccgttt
     1021 ataggtttac aaacatccgc tggagaagtg gatttagcta gtttaatcac gcaaaaggat
     1081 aaattggtga gcgaacttcg gaatcaaaaa tatatggatt taattgatga atataatttt
     1141 gatttaatta aaggtgaagc aaaattcgtt gatgctagta cggttgaggt caatgggaca
     1201 aagttatctg caaaacgctt tttaattgca acaggtgcat cgccttcgtt gccccaaatt
     1261 tcaggacttg aaaaaatgga ctatttaact agtacaacac ttcttgagtt aaagaaaata
     1321 ccaaaacgat taactgtaat tggttcagga tacattggaa tggagcttgg acaactattt
     1381 catcatttag gttcagaaat aacgcttatg caaagaagtg agcgactttt aaaggagtat
     1441 gatcctgaga tttcagagtc agttgaaaaa gcgttaattg aacagggtat aaaccttgtc
     1501 aaaggggcaa cttttgagcg tgttgaacaa agtggagaga taaaaagggt ttacgtaaca
     1561 gtaaatggca gtagagaagt cattgaatca gatcagttac ttgttgccac tggaagaaaa
     1621 ccaaatacgg attctttaaa tttaagtgca gcaggtgttg aaactggaaa aaataatgaa
     1681 atcctgatca atgattttgg tcaaacaagt aatgaaaaga tttatgcagc aggagatgtg
     1741 actttaggac cacaatttgt atatgtagca gcctatgaag gtggaattat tactgataat
     1801 gctattggtg gattaaacaa aaaaatagat ttatcggtag ttcctgctgt tacgtttacg
     1861 aatccgacgg ttgcaacggt tggtttaaca gaagaacaag caaaagagaa agggtatgat
     1921 gtgaagacat ctgtattacc tttaggtgct gttccaagag caattgtaaa ccgtgaaaca
     1981 accggtgtat ttaaactagt agcagatgca gaaacactaa aagtattagg ggttcatatt
     2041 gtatctgaaa atgcaggaga tgtcatctat gcagcatcat tagctgttaa atttggctta
     2101 acgatagaag atttaacaga aaccctagca ccatatttaa caatggctga agggctaaaa
     2161 ttagttgcac ttacgttcga taaagatatt tcgaaattat cttgttgtgc aggctaaggg
     2221 aactttttta caactcccta ttcaagtgaa ccagctaatg ctttaaggga ttttctaagt
     2281 gcatttgtgg tcacaaaaat aatttaacac tgatgatttc gacatcaaat ctataattga
     2341 taccttaaca ccgcagagta ataaaggatg aataatatgt cagacttatt atccctacca
     2401 gacattaaaa caatagaacc gccacaagaa aatgaaaccg atatgatgtt taaagttgaa
     2461 gcagtcggac cacctgaacg ttgtcctgaa tgtggttttg acaagttgta caaacacagt
     2521 tcaagaaatc aactaattat ggatttgccc attcgtttaa agcgagtggg cttacatttg
     2581 aaccgtagac gatacaagtg tcgtgaatgc ggatctacca tatctgtaga tgaaaagcgt
     2641 agtatgacca aaaggctttt aaagtccatt caagagcaat ccatgtctaa gacctttgta
     2701 gaagtcgcag aaagcgttgg tgttgacgag aaaaccatta ggaacgtttt taaggactat
     2761 gtggcactca aagaacgtga ataccagttt gaaactccta agtggcttgg gatagacgag
     2821 atacatatta tccgtagacc tcggcttgta ttgactaata ttgaacgcag gactatttat
     2881 gacatcaagc ctaaccgtaa caaggaaaca gtcatccaac gtctttcaga aatcagtgac
     2941 aggacttaca ttgagtacgt cacaatggat atgtggaagc cctacaaaga cgcagtgaac
     3001 actatccttc cacaagctaa agttgtcgta gataagtttc atgtagttag aatggctaat
     3061 caagccttag ataacgtcag aaagtctttg aaagcccata tgagccaaaa agaaagacgt
     3121 acccttatgc gtgaaaggtt tatccttcta aagcgtaaac acgatctaaa tgaacgtgaa
     3181 tcattcctct tagatacttg gttaggtaat cttcctgctt taaaagaagc ctatgaactc
     3241 aaagaagagt tttactggat atgggatact cctgatccag atgaaggtca tcttcgttat
     3301 agtcaatgga gacaccgttg tatgtccagc aactctaaag acgcatataa agacctcgtg
     3361 agagccgtag acaactggca tgttgaaata ttcaactact ttgataaaag gctcactaat
     3421 gcttatacgg agtcaattaa cagcattatt aggcaggtag agcgaatggg tagaggttac
     3481 tcgtttgatg ccttacgagc caaaatcctt ttcaatgaga agctccataa aaagcgtaag
     3541 ccacgattta attcaagtgc tttcaataaa gctatgttat acgatacttt caattggtat
     3601 gaagtgaatg atcacgacat tacagactag tcaagtgtct gctgatcaag ttacaactca
     3661 agcaacaact caaacagtaa cgcaaaatca agcagaaaca gtaacatcaa ctcaacttga
     3721 taaagcagta gctacagcta aaaaagcagc tgtagctgtt acaaccacag ctgcagttaa
     3781 tcatgcgaca actactgatg cacaagctga tttagctaat caaacacaaa cagttaaaga
     3841 tgttactgca aaagcacaag ctaatacaca agctattaaa gatgctactg ctgaaaatgc
     3901 aaaaattgac gcagaaaaca aagcagagtc tcaacgtgta agtcagttaa atgcacaaac
     3961 taaagcaaaa attgatgctg aaaacaaaga tgcacaagct aaagcagatg cgactaatgc
     4021 acaattacaa aaagattatc aagctaaatt agcgaaaatc aaatcagttg aagcttataa
     4081 tgcaggtgta cgtcaacgta ataaagatgc acaagctaaa gcagatgcga ctaacgcaca
     4141 gttacaaaaa gactatcaag ctaaattagc actttataat caagctctaa aagctaaagc
     4201 agaagcagat aaacagtcta ttaataatgt tgcttttgat atcaaagctc aagctaaagg
     4261 tgttgataac gctgaatatg gaaactcaat catgactgca aaaactaaac ctgacggaag
     4321 tttcgagttt aaccatgata tgatcgatgg tgtgaagaca atcggctatg ggaaattgac
     4381 aggtaaagtt aatcatcatt atgttgctaa caaagatggc tctgtgacag catttgttga
     4441 ctctatcact ctttacaagt acgagtatcg taatgttgct caaaatgctg ctgttaatca
     4501 aaatattgct ttcagagtct taactaaaga cggacaaatc atttttgaga agaaacataa
     4561 tggtaacaaa acttttgcag aaactttaaa caaaacttta caactcaatc ttaaatatga
     4621 gcttaaacca catggttcta gtggaagcgt tgaagtcttt aagattcatg atgactgggt
     4681 acgtgacaca catgggtctg ctttagtgtc ttatgttaat aataatgatg ttgttcctaa
     4741 cgttgttata ccaaaacagc caacaccacc gtcaacagaa aaagtgactc ctgaagcaga
     4801 aaaaccagta cctgaaaaac cagttgagcc taaatttgta acacctacat taaaaactta
     4861 cactccagct caaccaaaag tgaaaccaca tgtgtctatt cctgaaaaga ttaactactc
     4921 agttagtgtt catcctgttt tagttccagc tgctaatcct tcaaaagctg tcattgatga
     4981 agcaggtcaa tctgttaatg gtaaaaccgt attaccaaat gctgaattaa attacgttgc
     5041 taaacaagac tttagccaat ataaaggaat gactgcatca caaggtaaaa tagctaaaaa
     5101 ctttgtcttt attgatgatt ataaagatga cgcactagat ggtaagtcta tgaaagtgaa
     5161 ctcaatcaaa gcatctgatg gcacagatgt gtcacaactc ttagaaatgc gacatgtgct
     5221 ttctacggat actttagatg agaaacttca aacgcttatt aaagaagcag gaatcagtcc
     5281 tgttggtgag ttttacatgt ggactgcaaa agatccacaa gctttttata aagcttatgt
     5341 tcaaaaaggt ctagatgtta cttataactt atcatttaaa gttaaaaaag agtttactaa
     5401 aggtcaaatt caaaatggtg ttgcacaaat cgactttggc aatggatata caggtaatat
     5461 tgtagtcaat gatttgacaa ctccagaaat acataaagat gtgttagaca aagaagatgg
     5521 caagtctatc aacaatggta ctgtcaaatt aggtgatgaa gttacttaca aattagaggg
     5581 ttgggtagtt ccaacaggtc gttcttatga tctatttgaa tacaaatttg tggatcaatt
     5641 acaacgcaca catgatcttt acttacgtga taaagtggtc gctaaagttg atgtgacatt
     5701 aaaagatggt acagtcatta aaaaagggac taatttagga gagtacacag aaacagttta
     5761 caacaaaaag acaggtctct atgaattagt cttcaagaaa gacttccttg aaaaagttgc
     5821 acgttctagt gaatttgggg cagatgattt tgtggtggtt aaacgtatca aagcaggtga
     5881 tgtttacaat accgcagact tttttatcaa tgggaataag gttaaaactg aaactgtggt
     5941 aacacatact cctgagaaac caaaaccagt tgaaccacaa aaagcaactc caaaagctcc
     6001 tgctaaagga ttacctcaaa ctggtgaagc aagcgttgcc cctcttacag cacttggagc
     6061 aatcatcttg tcagctatcg gtttagcagg atttaaaaaa cgcaaagaaa attaaaaagt
     6121 caatattcag aagcacttga aaaagtgctt cttattttta gaaaagagga aattatgaaa
     6181 caagaaacat tagtgttact tccaaatttt attggagatg caatttatga aggatttgaa
     6241 gctggacctg ctcaattaaa tggtaaaaaa gggtcttata caagacacat tttgtttaat
     6301 gaaaaacatg gtaagtttta tgtttataca ccagcatctt cacgtgtgat tggaaatgga
     6361 aaaatggtaa ctgttgaaaa accgttattg tttattgaca atacaattaa tggtagaaat
     6421 attgaacctg caaaaaatgt atgggctgaa aagcttattg aagtgaaata aggaaagtag
     6481 aggattagaa atatggcaaa aattggttta aactttggag aaaaattaca aattgcagat
     6541 aaggaatatc gtacctttaa tgatggattg gatgtaaatg ttttgttagg agatttaact
     6601 tggagacgat atgaagaacc agttcaattt acggaagatg atacgacaca accaccagat
     6661 cgatttggtc gttatccaca aaaaccaaca ggtgaagtga agtggaatga tattgcagta
     6721 tttagtactg ctcaaaatga aacaattttt gtttcaatta ctgatatgcc ttcttatgca
     6781 atcgaggatt taggtcttaa attgggtgac cctattgaat taagtgatgt tgttgtgaca
     6841 tggtctaacg tttcaggtgg tatgtttaaa atttttgcat catcacttaa gaaaaaggat
     6901 gttgcaaata atggaaacgc atcaaaaaat gaagcatcta aagttaataa ataaacggta
     6961 aaggaggggt aatttggctc gtttaatacc acaatataga gggattaagg taaaaccata
     7021 tatgaggtat atgagttatt accttttttc ttttctattt attttgtttt tgacacctgt
     7081 aggcgtgtat agttattact atttagattt gttaaaaatg atggataaaa tgtctatgat
     7141 cagtgtaggt acaggtttgt ttttagcatt ttttgtttct tggtatttga cttggttttt
     7201 gcaagaagct aatccattat ttaataaact agatcgatta aaaagaatgt ctaagttttt
     7261 atatgagaat ggctatgttt atgaaaaacg taaaaaatca aataagaaaa cgaagacaaa
     7321 atatcgtttt ccaaaagttt atgtgaaaca aggtaagtat gatttaagtg tttcatttga
     7381 aatggctgga ggaaaatttc aaaagaaatt taaggatatt ggtggagaat tagaagatac
     7441 ttttttcatg gattttatgg aaaaaactga tgatccacgc tttaaaattt acaaattagc
     7501 ttattcggct ttcttatcac gtattacggt taaagatgtg atatgggata aagataaggg
     7561 aattaagtta atggatggct attattggga tttcatcaat gatcctcatt tattagttgc
     7621 tggtggtact ggtggaggta aaacagtatt actaaggtca atccttcgtt gtcttgcgga
     7681 aattggagtt tgtgatatat gtgaccctaa aagagccgat tttgttacta tgtcagattt
     7741 atcagctttt gaaggtagaa ttgcctttga aaaggctgat attattgaga agtttgaaaa
     7801 tgctgttacg ataatgtttg cacgttatga ttttgttcga aatgaaatga agcgtttagg
     7861 tcataaagac atgaaaaagt tttatgatta tggattggaa ccttattttt ttgtatgtga
     7921 tgaatataat gctttaatga gttctttgtc ttatcaggaa cgtgagattg tagataatgc
     7981 atttactcaa tatatattgt taggacgtca ggttggatgt aatgcgatta tagcaatgca
     8041 aaagccgtct gcagatgatc ttcccactaa aattagatct aatatgatgc accatatctc
     8101 ggttggtcgt ctcgatgatg gtggttatgt gatgatgttt ggtgatgaaa atagaaataa
     8161 ggaatttagg tttattaaat atcttgcagg tcgtcgtgtt tatggtcgag gttattcagc
     8221 tgtttttggt gaagtagcaa gagaatttta ttctccttta ttacctaaaa atttctcttt
     8281 ttatgatgct tttgaaaaaa taaataggca tgagaatcct tttgatccta cagaaaatca
     8341 ggaagtttct aaggcgattc ttaaagacga atctttgaga gagtttgtgg aaaaaacgtc
     8401 tgaaaatgag ttacttaagg gttcagtagg gtttgatttt gatgatgaaa tggaggaaat
     8461 ggaaaatatg agtattggtg atttagctaa gaaaacaggt ttttcatttg gtaagattaa
     8521 gaatttaata gatatgattg aaaaagataa ttatatgact tttagtcgtg aagatgaaaa
     8581 ggttgtatta aatgattctg atgcagtcgt cttagaaagt ttactgacac aaaaagatga
     8641 atttgagggt agctggaaag agttactatt gaattatttt aagggtgaat agtagatgaa
     8701 gggtattttt taaatatcct tctgtaaagc cctagtgtag ctgtaggcgt agtgtagcgt
     8761 agcgaaaacg gacacgccgg acacagcgca cacactaggg ctttacagag ggatttatca
     8821 attttaaaac ccccgttttc taataggggg gtaagaaatg aaaaatttcg aattttaacc
     8881 cccaaaaacc tatatgtatc aagggttttc agatttttaa aggtgggtca ttttagtgaa
     8941 aaaagtgggt cactaatcct aagaaggagt ttgtttattg tggatgctat ctatttaaaa
     9001 aaatttcgaa agaaaacagg tctaaaacaa aaggaatttg ctttaagttc tggtttgaca
     9061 ttgaaatcac ttagaaacta tgagcaagga aagcggaagt tgacacttga aaagtatcaa
     9121 gagataaaat cacattttgg atatctagta gaaaatgata gttcacgatt acaagtgatg
     9181 attgactatg tcagaattac gttaaaggat gttagggatc ttgaattttt ctgtcgtaac
     9241 tttttgcact gtgcatttaa agaatttcag ccttttgaat cgaagttgat gaattacaat
     9301 catctttgga aacgtggaga tatttggata tttgattttg cggataaaca tgagacaggt
     9361 aattttcaaa taactgttca attgtctggt cgtggatgcc gacaattgga attgttgatg
     9421 gaaactgaaa aatttacatg gcatgattgg ttaagttatc taagaaattc atatcgtgat
     9481 gatatgaatg tgacacgttt tgatattgct attgatgaat tgtatctggg taaggataga
     9541 gaaaatgaac agtttcattt gtcagatatg atttcaaaat attatcgtca tgagttagat
     9601 tttgaaagtt tgagaacttg gaattatata ggtggcggtt ctttaaattt ttcggatatg
     9661 gaagagattg agcagaatag acaaggaata tcgctttatt ttggtagtcg tcaatcagaa
     9721 atgtatttta acttttatga aaaacgttat gaaattgcaa agcaggaagg aattactgtt
     9781 gaagaagctt tagaaatttt tgaattgtgg aatcgttatg agatacggtt atctcaaagt
     9841 aaagctaatg cggcagttga tgaatttatt tctggagtgt cgattggtga aatatccaga
     9901 gggttaattg ttagtaagat tgacgtgtat gatgggaaaa atgaatatgg gtcgtttcaa
     9961 gcagatagaa agtggcaatt aatgtttgga ggtgttgagc ctttaaaatt tgttacaaag
    10021 ccagaggctt atagtattga acgaacctta cgttggttaa gtgattctgt ttctccgtct
    10081 ttagctatga taagagagta tgacatgatt gtggatggtg actatttaca aaccattctt
    10141 aattctgggg aagtcaatga acgtggggag aagatacttg acagtatcaa agctagctta
    10201 ggtattttgt aggaggtaag ttttgttttg tatagtaacc gagaatttgc ttattttgtt
    10261 aatagacgaa ataatttaga taaaatgatt gatttactag tttttatgat tcctgatcgt
    10321 gagttttatt atcctgaaat taatcaaggt gaattaagtg actatcaaaa agatattttt
    10381 gatctcattc aatttggcta ttgtggtgtc tatgaagtta agaaagaata tgaagataaa
    10441 cttcaacagt tagcaacatt gaaacgaaag ttacttaagt ttggtttgct tatgcaacct
    10501 ttagaaaagc aaaaagagat agttattcgt ttagctggta aatatcgttt ggaaaaaaga
    10561 attctgatga gaagggaaat gtttagagat gaggaagttg actaattttg gagaaaactt
    10621 aaggctaacg agaaaaacaa aaagtctaac tcaaactgag ctagcgtcta aattaaatat
    10681 aagtcaaaag tcatatagta attgggaaac agggaaaagt gaaccgaatt tagaaaatgt
    10741 cgtcaagcta gcaaaattat ttaaaacaac tacagatttt ttattggggg taacagatga
    10801 ttgaaaattt tgcacttaat ttaattcgtt tacgcaaaca aaaaggtcta tctcaaaaag
    10861 aacttgccaa aaaaatagga atgttaccgc aaaccatttc aaatattgaa aatcaaaaag
    10921 gctatcctac attttcaaac ttggataaaa ttgctcgata tttcaatgtc tcggcaacgg
    10981 aactttttgg aacaccacaa caaattgaat tagaaagagt aatgtttcaa actgatgaat
    11041 atagccgtaa agcagaaaag attttgtctg ctgtttcggt ttttgaaaat tttgtaaaga
    11101 aatatgatta tcaaaagatt atagaatttg gtttgagttt aaaagaataa aaaatacaaa
    11161 agtaagtagg ctacccctac tctttttatt tttggaggaa attatgttta ttagaagtcc
    11221 aactaaagtt agaaacaatt tgtaaattat taatattcaa tagaaaaaga ggaaaagaaa
    11281 aatgtcaaat acacgttcag aagcagataa aaagttgtta gtggttactc aggagctatc
    11341 agaactgttg actagtcatc agtatgaaca gtcatgggaa aaagctggag agttaaatag
    11401 cttacttaag agacgtgagg aactcacttt accaggttat atggtcgata tgattcaaca
    11461 acatttgaag tcatactact accaaaataa tatgattaat aaggctcata agtctatgtc
    11521 agcgattggt cataaactac aagaatttca ttaagattga gagcgagtca atgacttgct
    11581 cctttttaat taaggagtgt caaaaatgga tgttagtgca attgttagag atataaaaac
    11641 tggtagagct acgttagttt gttttcctgt ggagatgaat gagcttaagt tagctttggg
    11701 tcttcgtgaa gaagatgatt tagagtatat tattgcagat tcagattgtc agttactaaa
    11761 agaacatgat tctattgaga tgattaatca atttgttgaa ctggtagaga atgttgatag
    11821 tgaacttgtt aaagcagtgc atcaggtaat aggttatact gcctcagact ttgtggatta
    11881 tgattttaat tttggtgatt gttgtttact gtctgatgtc acaactagac gagaactagg
    11941 tgaatattat ttcgatgaat taggcgtaca aggagttggt aaagaggctc tggagatgta
    12001 ttttgatcat gaagcttatg gtcgagatat tgacttagaa agccaaggtg gtttttcaga
    12061 ctacggatat gtcgaaatat cagggtaagt agataatgat tgcaattata aggactcgtg
    12121 aaaaaggtag aagtcaattt ttatgtgagt tagacatcat gcagtttacg gaaaatcaag
    12181 taagaaaccg tatgatagaa aaaggcatta aggatgatgc cttttttatt tgtggttttt
    12241 ctgattggaa tgttgatagg attatgtcac ttacagaagt ctatctttta aaaagatgta
    12301 ttgaaggact ttatgatggt gatgactata ttgttcaata tcttttgaaa aaaggtttat
    12361 cagttcattc aatagtgaca aaatattata tatttttgtc aaataatgaa aataaagtaa
    12421 tgaaatatat cttaaggaaa gttgaatttg attctttaat tgatttttgg caacggtcag
    12481 taacttgggt aaatgcttta aatgcttata ttgaagaagg aatagtatta aacacctcaa
    12541 aaggttttta cgtgttaaaa acagaatgag gaggtaattg gaagc
//
LOCUS       DATRMA010000054        12565 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.054, whole genome shotgun sequence.
ACCESSION   DATRMA010000054 DATRMA010000000
VERSION     DATRMA010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12565
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.054"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            complement(47..1738)
                     /locus_tag="VBC28_001455"
     CDS             complement(47..1738)
                     /locus_tag="VBC28_001455"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEO6815434.1"
                     /translation="MDTKHLIASEIQKVVPDMEQSTILSLLETPKNSSMGDLAFPAFS
                     LAKTLRKAPQIIASDIAEQIKSDQFEKVEAVGPYVNFFLDKAAISSQVLKQVLSDGSA
                     YATQNIGEGRNVAIDMSSPNIAKPFSIGHLRSTVIGDSLANIFDKIGYHPVKINHLGD
                     WGKQFGMLIVAYKKWGNEEAVRAHPIDELLKLYVRINAEAETDPSVDEEAREWFRKLE
                     ANDPEATELWQWFRDESLLEFNRLYDQMNVTFDSYNGEAFYNDKMDEVLELLESKNLL
                     VESKGAQVVNLEKYGIEHPALIKKSDGATLYITRDLAAALYRKRTYDFAKSIYVVGNE
                     QSAHFKQLKAVLKEMDYDWSDDMTHVPFGLVTKGGAKLSTRKGNVILLEPTVAEAINR
                     AASQIEAKNPNLADKDKVAQAVGVGAIKFYDLKTDRTNGYDFDLEAMVSFEGETGPYV
                     QYAHARIQSILRKANFSPSNSDNYSLNDVESWEIIKLIQDFPRIIVRAADNFEPSIIA
                     KFAINLAQCFNKYYAHTRILDEDAEISSRLALCYATATVLKESLRLLGVDAPNEM"
     gene            1960..2397
                     /gene="argR"
                     /locus_tag="VBC28_001456"
     CDS             1960..2397
                     /gene="argR"
                     /locus_tag="VBC28_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEO6815435.1"
                     /translation="MNKIERQKRIKRLIQSGQIGTQEEIKLHLKNEGIDVTQATLSRD
                     LREIGLLKLRSPEGKLYYSLSTATSNRFSPALRSYILKVSRASFMLVLNTNLGEASVL
                     ANFIDEKGLPEILGTMAGADTLLVICQNEDIAKVFEKELSVGL"
     gene            2454..5030
                     /gene="mutS"
                     /locus_tag="VBC28_001457"
     CDS             2454..5030
                     /gene="mutS"
                     /locus_tag="VBC28_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEO6815436.1"
                     /translation="MAKPTISPGMQQYLDIKENYPDAFLLFRMGDFYELFYDDAVKAA
                     QILEISLTSRNKNAEKPIPMAGVPYHSAQQYIDVLVELGYKVAIAEQMEDPKKAVGVV
                     KREVVQVVTPGTVVESTKPDSANNFLVAIDSQDQQTFGLAYMDVSTGEFQATLLTDFE
                     SVRSEILNLKAREIVVGYQLTDEKNHLLTKQMNLLLSYEDERLNDIHLIDEQLTDLEI
                     SAAEKLLQYVHRTQKRELSHLQKVVHYEIKDYLQMSYATKNSLDLLENARTSKKHGSL
                     YWLLDETKTAMGTRMLRTWIDRPLVSMNRIKERQDIIQVFLDYFFERNDLTESLKGVY
                     DIERLASRVSFGKANPKDLLQLGQTLSQIPRIKMILQSFNQPELDIIVNKIDTMPELE
                     SLINTAIAPEAQATITEGNIIKSGFDKQLDNYRTVMREGTGWIADIEAKERAASGIGT
                     LKIDYNKKDGYYFHVTNSNLSLVPEHFFRKATLKNSERYGTAELAKIEGEMLEAREQS
                     SNLEYDIFMRVRAQVESYIKRLQELAKTIATVDVLQSLAVVAENYHYVRPKFNDQHQI
                     KIKNGRHATVEKVMGVQEYIPNSIYFDSQTDIQLITGPNMSGKSTYMRQLALTVIMAQ
                     MGGFVSADEVDLPVFDAIFTRIGAADDLISGQSTFMVEMMEANQAVKRASDKSLILFD
                     ELGRGTATYDGMALAQSIIEYIHDRVRAKTMFATHYHELTDLSEQLTRLVNVHVATLE
                     RDGEVTFLHKIESGPADKSYGIHVAKIAGLPIDLLDRATDILSQLEADAVQLIVSPSQ
                     EAVTADLNEELDSEKQQGQLSLFEEPSNAGRVIEELEAIDIMNLTPMQAMNAIFDLKK
                     LL"
     gene            complement(5087..5290)
                     /locus_tag="VBC28_001458"
     CDS             complement(5087..5290)
                     /locus_tag="VBC28_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000191849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEO6815437.1"
                     /translation="MTQGTVKWFNSEKGFGFISSETGTDVFAHFSEIKVDGFKTLEEG
                     QKVTFDIQDGQRGPQATNINLVK"
     gene            5510..7489
                     /gene="mutL"
                     /locus_tag="VBC28_001459"
     CDS             5510..7489
                     /gene="mutL"
                     /locus_tag="VBC28_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001054073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEO6815438.1"
                     /translation="MNLSKIIELPDILANQIAAGEVVERPSSVVKELVENAIDAGSSQ
                     ITIEVEESGLKKIQITDNGEGMTSEDAVLSLRRHATSKIKSQSDLFRIRTLGFRGEAL
                     PSIASISLMTIKTATEQGKQGTLLVAKGGNIEKQEVVSSPRGTKILVENLFFNTPARL
                     KYMKSLQSELAHIIDIVNRLSLAHPEVAFTLINDGKEMTKTSGTGDLRQAIAGIYGLN
                     TAKKMIEISNADLDFEISGYVSLPELTRANRNYITLLINGRYIKNFLLNRSILDGYGS
                     KLMVGRFPIAVIDIQIDPYLADVNVHPTKQEVRISKERELMSLISTAISESLKQYDLI
                     PDALENLAKTSTRSVDKPIQTSFSLKQPGLYYDRAKNDFFIGADTVSEPIANFTNLDK
                     SDGSVDNDVKNSVNQGATQSPNIKYASRDQADSENFIHSQDYLSSKQSLNKLVEKLDS
                     EESSTFPELEFFGQMHGTYLFAQGNGGLYIIDQHAAQERVKYEYYREKIGEVDNSLQQ
                     LLVPFLFEFSSSDFLQLQEKMSLLQDVGIFLEPYGNNTFILREHPIWMKEEEVESGIY
                     EMCDMLLLTNEVSVKKYRAELAIMMSCKRSIKANHTLDDYSARHLLDQLAQCKNPYNC
                     PHGRPVLVNFTKADMEKMFKRIQENHTSLRDLGKY"
     gene            7521..8753
                     /locus_tag="VBC28_001460"
     CDS             7521..8753
                     /locus_tag="VBC28_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO6815439.1"
                     /translation="MKEFFALPKQLQLRELLRFISITVGSAIFPFMAMYYVQYFGNLV
                     TGILIIITQLSGFVATLYGGHLSDAMGRKKVVIIGSLLATIGWAITIAANVPNHITPH
                     LTFVGILIIEIAHQFYFPAYEAMTIDLTNEQNRRFVYTIGYWLVNIAVMLGSGIAGIF
                     YDHHFFELLIVLLIISAICCFVVYFKFDETKPQEGTFKHDKGVLGTFKNYSQVLVDKA
                     FVVYTLGAIGSSVVWLQVDNYFSVNLKQNFEVVSILGHTITGAKMLSLAVFTNTLLIV
                     LLMTTINKFIENWPLKRQLILGSLICGFGMLFNISLNTFGAILIAMTFFTFGEMIYVP
                     ASQVLRAEMMVEGKIGSYSGFLAIAQPVASVLAGAMVSLSYFTGKIGVQITLTIFMLA
                     GLVLILYATKMKNIEIGK"
     gene            8755..9345
                     /gene="ruvA"
                     /locus_tag="VBC28_001461"
     CDS             8755..9345
                     /gene="ruvA"
                     /locus_tag="VBC28_001461"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEO6815440.1"
                     /translation="MYDYIKGKLSKITAKFIVVETAGLGYMIYVANPYSFSGYVNQEV
                     TIYLHQVIRDDAHLLFGFHTENEKEIFLNLISVSGIGPTTALAIIAVDDNEGLVSAID
                     NSDIKYLTKFPKIGKKTAQQMILDLSGKFVEASGESATSRKVSSEQNSNLEEAMEALL
                     ALGYKATELKKVKAFFEGTNETVEQYIKSSLKMLMK"
     gene            9368..9919
                     /locus_tag="VBC28_001462"
     CDS             9368..9919
                     /locus_tag="VBC28_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEO6815441.1"
                     /translation="MKRCSWVNLDNPLYVAYHDKEWGRAVHDDHVLFELLCLETYQSG
                     LSWETVLNKRQEFRQVFHHYNIEKVAAMSDADLEIILQNPRVIRHRLKLFSTRQNARS
                     IILIQKEFGSFDRYIWSFVDNKVQVNSVNNYNDVPASTTLSERLSKDLKKRGFKFVGP
                     TCLYSFIQAAGMVNDHENICDFK"
     gene            10008..11268
                     /locus_tag="VBC28_001463"
                     /pseudo
     CDS             10008..11268
                     /locus_tag="VBC28_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503342.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
     gene            11342..12481
                     /gene="recA"
                     /locus_tag="VBC28_001464"
     CDS             11342..12481
                     /gene="recA"
                     /locus_tag="VBC28_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001085741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEO6815442.1"
                     /translation="MAKKTKKAEEITKKFGDERRKALDDALKNIEKDFGKGAVMRLGE
                     RAEQKVQVMSSGSLALDIALGAGGYPKGRIVEIYGPESSGKTTVALHAVAQAQKEGGI
                     AAFIDAEHALDPAYAAALGVNIDELLLSQPDSGEQGLEIAGKLIDSGAVDLVVVDSVA
                     ALVPRAEIDGDIGDSHVGLQARMMSQAMRKLSASINKTKTIAIFINQLREKVGVMFGN
                     PETTPGGRALKFYSSVRLDVRGNTQIKGTGEHKDHNVGKETKIKVVKNKVAPPFREAF
                     VEIMYGEGISRTGELIKIASDLDIIQKAGAWYSYNGEKIGQGSENAKKYLADNPAIFD
                     EIDHKVRVHFGMTEDDSPVQSELVEEKNEADDLVLDLDNAIEIEE"
BASE COUNT         4082 a         1978 c         2589 g         3916 t
ORIGIN      
        1 aaaattaaga ctgtcataaa tttgacagtc ttagtagtgt catttcttac atttcattcg
       61 gagcatcaac tcctaggagg cgaagagatt cttttaatac tgtagcagtt gcatagcaaa
      121 gtgctagacg actactgatt tcagcgtctt catctaaaat acgtgtgtgg gcgtaatatt
      181 tattgaagca ctgagctaaa ttaatagcga attttgcaat aatagagggc tcaaaattat
      241 ctgccgcacg tacaataata cgaggaaaat cttggatgag tttgatgatt tcccagcttt
      301 caacatcgtt gagactgtaa ttatcgctat tagatgggct aaagttagct ttacgtaaga
      361 ttgattgaat acgagcatgt gcatattgta catatggccc agtttcacct tcaaatgata
      421 ccattgcttc taagtcaaag tcataaccat ttgtacgatc agttttcaaa tcgtaaaatt
      481 taatagcacc tacaccaact gcttgagcaa ctttatcttt atctgctagg ttagggtttt
      541 tagcttcaat ctgagatgcc gcacgattaa tagcttctgc aacagtaggt tcaagtagaa
      601 ttacatttcc tttacgagtt gataattttg ctcctccttt tgttacaagg ccaaaaggaa
      661 catgagtcat atcatcggac caatcgtagt ccatttcttt aagcacagct tttagttgct
      721 taaagtgtgc agactgttcg tttcctacaa cgtagattga ttttgcaaaa tcgtaggtac
      781 gtttacggta aagtgcagca gctaagtcac gtgtaatgta gagagttgct ccatcggatt
      841 ttttaatgag ggcaggatgt tcaataccgt atttttctag gttaacaact tgtgcacctt
      901 tagattcgac taggaggttt ttagattcta gcagttcaag cacttcatcc attttgtcgt
      961 tgtagaatgc ctcgccgtta tagctatcaa atgtaacatt catttgatcg taaagacggt
     1021 tgaattctaa taatgactcg tcacggaacc attgccataa ttcagttgct tcaggatcgt
     1081 tagcttcaag tttacgaaac cactcacgtg cctcttcatc aacgctaggg tctgtctcgg
     1141 cttcagcatt aatacggaca taaagtttta aaagttcatc gatgggatga gcacggacag
     1201 cctcttcatt gccccatttt ttataggcaa cgattaacat tccaaattgt ttaccccagt
     1261 cgccaaggtg attaatttta acaggatgat agccaatttt atcaaaaata ttagctaaac
     1321 tatcaccaat aactgttgaa cgaaggtgtc caattgagaa tggcttagca atatttggac
     1381 tagacatgtc aatggcaaca tttcgtcctt caccaatatt ttgagtagca taggcagaac
     1441 catcagataa gacttgtttt aaaacttgac ttgagattgc ggctttatca aggaaaaagt
     1501 taacgtaagg tccaacagct tccacctttt caaattggtc gcttttaatt tgttcagcaa
     1561 tgtcactagc aattatttga ggtgctttgc gtagagtttt agccaaagag aatgctggga
     1621 aagctaaatc tcccatgctc gaattttttg gggtttctaa taaagaaaga atggtcgatt
     1681 gttccatatc tggaacaact ttttgaattt cactcgcaat tagatgtttt gtatccatgg
     1741 tatctcctat tttaattagt aatatagtca ccctatttta acataattat gaaaaataga
     1801 cgagaacttg ttgattaaat tcataatgca ttctgtaaag aactagccta ctttatagct
     1861 tttttacaga atgattacga tatcactact aagtgaaaaa gacaattttt tttgatataa
     1921 tttggtaagc ataattctaa ctaaaaagga ttgaataaca tgaataaaat agaacgccaa
     1981 aaacgtatta aacgtttgat ccaatcaggg cagattggca cacaagaaga aattaaatta
     2041 catttaaaaa atgaaggaat cgatgtcacg caagcgactc tatcaagaga cttgcgtgag
     2101 attggtcttt taaaattacg tagcccagaa ggtaagctat attatagttt atcaacagct
     2161 acttcaaatc gatttagccc agccttacgc tcatatattt tgaaagtatc gcgagcaagt
     2221 tttatgttag ttttaaatac taatttaggg gaggctagcg ttttagcaaa ctttattgat
     2281 gagaaagggc tgccagagat tttaggaaca atggcaggag cagacacctt gttggtgatt
     2341 tgtcaaaatg aagatattgc caaagttttt gaaaaagaac tatcagtagg tttataaata
     2401 taatgattga ggagctgagc atggcagcca gccccgttag ttaggagtga gaaatggcca
     2461 agccaacgat atcaccggga atgcaacagt atctggatat aaaagagaat tatccagatg
     2521 cttttttgct ttttagaatg ggtgattttt atgaattatt ttatgatgat gcggtaaaag
     2581 cagcacaaat cctggaaatt agcttgacta gtcgaaataa gaacgcagaa aagccaatcc
     2641 caatggcagg agttccctat cactcagctc aacagtatat tgacgtttta gttgaattag
     2701 gttacaaagt agccattgct gagcagatgg aagatcctaa aaaagctgtg ggagtggtca
     2761 agcgtgaggt agtgcaagtt gttaccccag gaacggttgt ggagtcaacg aagccggata
     2821 gtgctaataa tttcttagta gcgattgatt cgcaagatca acaaacattt ggtctagcat
     2881 atatggatgt ctcaactgga gagtttcagg caaccctttt aacagatttt gagtccgtcc
     2941 gtagtgaaat actaaattta aaagctcgtg agattgtagt aggatatcaa ttgacggacg
     3001 aaaaaaatca cctactgacg aagcagatga acttgctttt atcatacgaa gacgaacgac
     3061 ttaatgatat tcatttgatt gatgagcagt taactgattt ggaaatatct gctgcggaaa
     3121 aacttttaca atatgtgcat agaacacaaa agcgtgaact tagtcattta cagaaagtag
     3181 ttcattatga aataaaggac tatttacaaa tgtcatatgc aacgaaaaat agtctagatt
     3241 tactggagaa tgctagaaca agcaagaagc atggaagtct ttactggttg ttagatgaga
     3301 ctaaaacagc gatgggaact cgaatgctga gaacttggat tgacaggcct ttggtaagta
     3361 tgaatcgaat caaggaacgt caagatatta ttcaagtgtt tcttgattat ttttttgaga
     3421 gaaacgatct cacagaaagt ttaaagggtg tatatgatat tgaacgccta gcaagtcgag
     3481 tatctttcgg aaaagccaac cctaaagatc tattgcaact cggacagacc ttatcacaaa
     3541 ttcctcggat taaaatgatt ttacagtcct tcaatcaacc tgagcttgac atcattgtca
     3601 acaaaattga cactatgcct gaattagaaa gtttaattaa tacggcgata gccccagaag
     3661 cacaggctac tatcactgag ggaaacatta tcaagtctgg atttgataag caattggata
     3721 attatcgaac agtgatgcgt gaaggtacag gttggattgc tgatattgaa gctaaggaaa
     3781 gagcagcaag tggtatcggt actcttaaaa ttgattataa taaaaaagac ggttattact
     3841 tccatgttac caattccaat ttatcactag taccggagca ttttttccgt aaagcgacat
     3901 taaaaaattc tgaacgctat ggaacagcag aactagccaa aattgaaggt gaaatgctcg
     3961 aagctcgcga gcaatcttca aatttagaat atgatatttt tatgcgtgtt cgtgcccaag
     4021 tagaatctta tattaaacgt cttcaagagt tagcaaagac gattgcaacc gttgatgttc
     4081 tacagagttt ggcagtagtt gcagaaaatt atcactatgt tcgtcccaaa tttaatgatc
     4141 aacatcagat taagattaag aatgggcgtc atgcaactgt tgaaaaagtg atgggagtgc
     4201 aagaatatat tcccaatagc atctattttg atagtcagac agatatccag ttgattacag
     4261 gaccaaatat gagtggtaag tcgacctata tgcgccagtt agctttgaca gttattatgg
     4321 cacaaatggg aggttttgta tcggcagacg aagttgattt gcctgtattt gatgcaatat
     4381 ttactaggat tggtgctgct gacgacttaa tttctgggca atcaaccttt atggtagaaa
     4441 tgatggaagc gaatcaagct gtaaaacgag ccagtgataa atctttgatt ctttttgatg
     4501 aattaggtcg agggacagcc acttatgatg gtatggcatt agctcaatcg attatagaat
     4561 atattcatga ccgtgttagg gcaaaaacaa tgtttgcgac tcattaccat gagttgacag
     4621 atttatctga acagttgaca aggcttgtca atgtacacgt ggctacttta gagagagatg
     4681 gagaagttac cttcttacat aaaattgaat ctggacctgc ggataagtct tatgggatac
     4741 acgtcgcaaa aatagctggt ttaccaattg acttattgga tagggcaact gatattttat
     4801 cacagttgga agctgatgca gtacagttga tcgtatcgcc ctcccaagaa gctgttactg
     4861 ctgacttaaa tgaggaacta gattctgaga agcaacaagg acaattatcg ctttttgaag
     4921 aaccttcaaa tgcaggtagg gttattgagg agttagaagc gatagatata atgaatctaa
     4981 ctccaatgca agctatgaat gctatatttg acttaaagaa attattataa aatagccttt
     5041 aggcaacaaa aaagagacta aataattcat ttaggctctt ttttaattat ttaacaagat
     5101 tgatatttgt tgcttgtggt ccacgttgac catcttgaat atcaaatgtt actttttgtc
     5161 cttcttcaag agttttgaag ccatcaactt taatctctga aaaatgagcg aagacgtctg
     5221 ttccagtttc gctagaaata aagccaaaac ctttttctga gttaaaccat ttcactgtac
     5281 cttgtgtcat ttgtttatta cctttctaaa aatgttactg taaaaaataa aaaaggtaaa
     5341 aatgttaaac aaaaatacaa cttcaaaact tattacgctc cctaagtcta acaggtaata
     5401 ggagtgatgt caactaaaaa ttgtgttatt gtcaaattaa tattttgaat atggtataat
     5461 ggctaagata tcacaaaaaa agttacaaaa aatcataaaa aaaggaggga tgaacttgtc
     5521 taaaattatt gaattaccgg atatattagc taaccagatt gctgctggtg aggttgttga
     5581 aaggcctagt agcgtcgtta aagaattagt tgaaaatgcc atagatgcag gaagtagcca
     5641 aattaccata gaggttgaag aatctggtct taaaaagatt caaataacgg ataacggtga
     5701 agggatgact agtgaggatg ccgttctcag tttacgacgt cacgctacat ccaaaatcaa
     5761 aagccagagt gacctttttc gtattcggac cttaggtttt cgtggtgaag ctctaccatc
     5821 aatcgcttcg attagtttaa tgacgataaa aacagctaca gagcaaggga aacaagggac
     5881 gttacttgtt gctaaagggg gaaacattga aaaacaggaa gttgtttcaa gtcctcgtgg
     5941 aacaaaaatt ttagttgaaa atttattctt taacacgcct gccagattga aatacatgaa
     6001 gagtttacag tctgaactag ctcatattat tgatattgtt aatagactca gtctagcgca
     6061 tccggaagtt gcttttacac tgataaatga tggtaaagaa atgactaaaa ctagtgggac
     6121 tggcgatttg agacaggcga ttgcaggtat ttatggttta aatactgcaa aaaaaatgat
     6181 tgagatttca aatgcagatt tagattttga aatttcgggt tatgtaagtt tgcctgaatt
     6241 gacacgagct aaccgaaact atattaccct attaattaac ggtcgttaca ttaaaaattt
     6301 tcttttgaac cgctcaattc tagatggtta tggttcaaag ttgatggttg gaagatttcc
     6361 aattgctgtc attgatattc agatagatcc ttatctagca gatgtcaacg ttcatccgac
     6421 taagcaagag gtgagaattt ctaaggaacg agaattaatg agtttaataa gcacagcaat
     6481 ttctgagagt cttaaacaat atgacttgat tccggatgct ttagagaatt tagctaaaac
     6541 tagtacccga agtgtagata agcctataca aacaagcttc tcactaaaac agcctggttt
     6601 gtattacgat agggctaaaa atgacttttt tataggtgca gatactgtat ctgaacctat
     6661 tgctaacttt acaaaccttg acaaaagtga cggttcagtt gacaatgatg taaaaaactc
     6721 tgttaatcaa ggagcaacgc agtctcctaa tataaagtat gctagtagag atcaagctga
     6781 tagtgagaac ttcattcatt ctcaggatta ccttagtagt aaacagtcac ttaacaaact
     6841 tgttgaaaaa ttagattcgg aagaaagttc gacttttcca gagctcgaat tttttggtca
     6901 aatgcatggg acctacttat ttgctcaagg gaatggtggg ttgtatatta ttgatcaaca
     6961 tgcagctcaa gagcgtgtaa aatacgagta ttaccgtgaa aagataggtg aggttgataa
     7021 tagtcttcaa caattgttag taccatttct atttgaattt tcaagttcag attttcttca
     7081 attacaagaa aaaatgtcat tattacaaga tgttggtatt ttcttggaac cgtatggtaa
     7141 taatactttc attttaaggg aacatccaat ttggatgaaa gaagaagagg tagagtctgg
     7201 catttatgaa atgtgtgata tgttgttgct cactaatgaa gtatcagtga aaaaatatcg
     7261 cgcagagttg gctattatga tgtcttgcaa gcgatctatt aaagcaaacc atacgctaga
     7321 cgattactca gcacgtcatt tattagatca gttagcgcaa tgtaaaaatc cttataattg
     7381 ccctcatgga agacctgttt tagttaattt tacaaaagct gatatggaga aaatgtttaa
     7441 acgaatccaa gaaaatcata caagcctcag ggatttagga aaatattgat aattacttgt
     7501 tagggaagaa gggagaaaaa gtgaaagagt tttttgcttt gccaaaacag ctacagttaa
     7561 gagaattatt gcgttttatt tccattacgg ttggttcagc tatttttcct tttatggcaa
     7621 tgtattatgt tcaatatttt ggaaatctag ttacaggaat cttgatcatc ataactcaac
     7681 tatcagggtt tgtagcgacc ttatatggag gccatttgtc agatgctatg gggaggaaaa
     7741 aagttgtgat tataggttca ctgctggcaa ccattggttg ggctataact atagctgcaa
     7801 atgttccaaa tcatattacg ccacacttga cctttgtagg tatccttatc attgaaatag
     7861 ctcaccaatt ttatttccct gcctatgaag ctatgaccat tgatttgacg aacgaacaga
     7921 atcgtcgctt tgtttacact ataggttatt ggttggttaa catcgctgta atgctaggtt
     7981 ctggaattgc tgggattttc tatgatcacc atttttttga attattaata gttcttctta
     8041 ttatttcagc gatttgttgt ttcgtggtct acttcaagtt tgatgagaca aaaccacagg
     8101 agggaacctt caagcatgat aaaggagttt taggaacttt taaaaattat agtcaggttt
     8161 tagtggataa agcgtttgtt gtttatactt taggagcaat tggttccagt gttgtctggc
     8221 tccaggttga taattatttc tcggtcaatc ttaagcaaaa ttttgaagta gtgtccattt
     8281 taggacatac aatcacaggt gctaaaatgc ttagtttagc agtctttaca aacactctat
     8341 taattgtctt actaatgaca actataaata aattcataga gaattggcct ttgaaacgtc
     8401 aactaatatt agggagcttg atttgtggtt ttggaatgct ttttaatatt tctctaaaca
     8461 cttttggagc tattttgatt gccatgacgt ttttcacctt tggcgaaatg atttatgttc
     8521 cagctagtca ggttctaagg gcggagatga tggtggaggg aaaaattgga tcatattctg
     8581 gatttttagc tatcgcacaa cctgttgcaa gtgtattagc aggtgccatg gtttcgctca
     8641 gctattttac aggaaaaata ggtgttcaaa taacattgac catttttatg ttagcaggtc
     8701 tggttttaat cttatatgct accaaaatga aaaatattga gataggaaaa tagaatgtac
     8761 gattatatta aaggaaaatt gagtaaaata acggctaaat ttattgttgt ggaaacagct
     8821 ggattgggat acatgatata tgtggcaaat ccctactctt tttcaggtta cgttaatcaa
     8881 gaagttacca tctacttaca tcaagtcatt cgcgatgatg cacatctttt atttggcttt
     8941 catacggaaa atgaaaaaga aatttttctt aatttaattt cagtatcagg aattggtcca
     9001 acgaccgctt tagcaattat tgcagtcgat gacaatgaag gtctcgtaag tgccattgat
     9061 aatagtgata ttaagtattt gactaaattt ccaaagatag gaaaaaaaac agcacaacag
     9121 atgatccttg atttgtcagg gaagtttgta gaagcttctg gtgagagtgc gactagtcgt
     9181 aaggttagct ctgagcagaa tagcaatctt gaagaagcta tggaagcttt gttagcgttg
     9241 gggtataagg cgactgaatt gaaaaaagta aaagcctttt ttgaaggtac gaacgagact
     9301 gtggagcaat acatcaaatc tagtttgaag atgttgatga aataatttag aagtaatgag
     9361 gtttcacatg aaacgatgtt cttgggtgaa tttagataat ccattatatg tagcttacca
     9421 tgataaagag tgggggagag ctgttcatga cgatcatgta ctctttgagt tactatgttt
     9481 agaaacttat caatcgggtt tatcttggga aacagtatta aataaacgtc aggagttcag
     9541 acaagttttt catcactata atattgaaaa ggtagctgca atgtcagatg cggatttaga
     9601 gataatctta caaaatccta gggttattcg tcatcgtcta aaacttttct caacacgaca
     9661 aaatgctagg tcaattatat taattcagaa agaattcgga agttttgatc gctacatttg
     9721 gtcttttgta gataataaag tacaggttaa ctcagttaac aactacaacg atgtgcctgc
     9781 aagtactacc ttatccgaac gcttatctaa ggatttaaaa aaacgcggtt ttaaatttgt
     9841 tggtccaact tgcctctatt cttttattca ggccgcgggt atggttaatg accatgaaaa
     9901 tatatgtgat ttcaagtaat atcaatgtga tatgttttaa gtagcacttt tgctactttt
     9961 tttgctaaaa tgtagtcatt atttcgaata gataagaggg tgaagttgtg agggctgaaa
    10021 ttatcgctgt aggaacagaa attttaacag gacagatcgt taatacaaac gcacaatttt
    10081 tgtcggagaa aatggcagaa ttgggaattg atatttattt ccagacagct gtaggagaca
    10141 atgaggaacg tttattatcg cttttagata tagctagtca gcgcagtcaa ttggttatcc
    10201 tatgtggtgg acttggccct acagaggatg acttaacgaa gcaaactttg gctaaatttt
    10261 taggtaaatc attaacagta gatttattag ctagtcgaaa attggatcgt tttttttgca
    10321 agtcgtcctc agtttgctag aacgccaaat aatgaacgac aagctcaatt ggttgaaggc
    10381 tcaatacctc tacaaaacct tactggtcta gctgttggtg gtatcgttac atctaaggga
    10441 gtccaatata tggttttacc aggtccgcct agtgaattaa agccaatggt tatggagcag
    10501 gtagtaccta ttttgtcaaa taatggcaca aaattatatt cacgtgtatt gagattcttt
    10561 ggtatcggtg agagtcagtt agtcacaatc ttagaagaca taattaagaa tcagacagac
    10621 ccaactatag caccatatgc aaaggttggt gaggtaactt taaggttatc aacgaaagct
    10681 gaaaatcagg atgaggccga ctttaaatta gatagcttag aaaaagaaat acttgctctt
    10741 aagacattag ataaccgtaa attaaaagat ttattatatg gttatggtga taataatagt
    10801 atggcacgta ctgttttaga attattgaaa gtacaaaata aaacgattac tgccgcagag
    10861 agcttaacag cagggttatt tcaatctcag ttagcagaat tttctggtgc ttcgcaagtt
    10921 tttaatggtg gttttacgac ctatagtatg gaagcaaaat cacaacttct cggcatccct
    10981 aagaaaaaat tgcaagaata tggagttgtc agtcatttta cagctgaggc aatggctcag
    11041 caagctcgcc aacttttaaa agcagatttt ggtatcggtt taacgggagt tgctggtcct
    11101 gatgagttgg aaggataccc tgcaggtaca gtttttattg ggattgcaac ccctgaaggc
    11161 gtttcttcta ttaaagtatc gattggaggg aagagtcgct cagatgtccg tcatatcagt
    11221 actttacatg cttttgactt ggtgcgtagg gctttattaa agatataata tttgttataa
    11281 ttagtagaac gatttctaaa tttgaatcaa cctagattca tagaaatata aggagaagac
    11341 attggctaaa aaaacgaaaa aagcagaaga aattactaag aaattcggtg atgaacgtcg
    11401 aaaagcctta gatgatgctt tgaaaaatat tgaaaaagat tttggtaagg gcgcagtgat
    11461 gcgcttaggt gagcgagctg aacaaaaggt tcaagtaatg agttcaggtt ctcttgcttt
    11521 agatatcgct ttaggagcag gtggatatcc taaaggacgt atcgtcgaaa tttatggacc
    11581 agagtcatct ggtaaaacaa cggttgccct tcatgccgta gctcaagccc aaaaggaagg
    11641 tgggattgct gcctttattg atgctgagca cgctcttgac ccagcctatg ctgcggctct
    11701 tggagttaat attgatgagt tacttttgtc tcaacctgac tcaggtgagc aaggtcttga
    11761 aattgcaggt aaattgattg attcaggcgc agttgattta gttgttgtcg attcagttgc
    11821 agcccttgtt cctcgtgctg agattgatgg tgatattggt gacagccacg ttggtttgca
    11881 agctcgtatg atgagtcaag caatgcgtaa attgtcagca tccattaaca agacaaagac
    11941 aatcgcaatt tttatcaacc agttaaggga aaaagtaggc gttatgtttg gtaaccctga
    12001 aacaacacct ggtggtcgtg ctttgaaatt ttattcatcg gttcgtctag atgttcgtgg
    12061 taatactcaa atcaagggaa ctggtgagca taaagatcat aatgttggta aagagacaaa
    12121 aatcaaagtt gttaaaaata aagttgctcc accattccgt gaagcttttg tcgaaattat
    12181 gtatggtgaa ggtatttctc gcacgggtga gttaattaaa attgctagtg atttggatat
    12241 tatccaaaaa gcaggtgcat ggtactcata taatggtgag aaaattggac aaggttctga
    12301 aaatgccaaa aaatatttag cagataatcc agcaattttt gatgaaattg accataaagt
    12361 gcgcgtgcac tttggaatga ctgaagatga ttcgccagtt cagtctgagc tcgttgaaga
    12421 aaaaaatgaa gctgatgatc ttgttttaga tttggacaat gctattgaaa ttgaggaata
    12481 aaatagcaac tatacatctt gaacttgaat aaatgtaact aaaataagtt ctgacagtgg
    12541 ttggaactta tttttatact aaaaa
//
LOCUS       DATRMA010000055        12438 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.055, whole genome shotgun sequence.
ACCESSION   DATRMA010000055 DATRMA010000000
VERSION     DATRMA010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12438
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.055"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            217..288
                     /locus_tag="VBC28_001465"
     tRNA            217..288
                     /locus_tag="VBC28_001465"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:249..251,aa:Gln,seq:ttg)
     gene            297..368
                     /locus_tag="VBC28_001466"
     tRNA            297..368
                     /locus_tag="VBC28_001466"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:330..332,aa:Glu,seq:ttc)
     gene            431..949
                     /gene="rimM"
                     /locus_tag="VBC28_001467"
     CDS             431..949
                     /gene="rimM"
                     /locus_tag="VBC28_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEO6815443.1"
                     /translation="MEYFNVGKIVNTQGLQGEMRVLSVTDFVEERFKKGQVLALFDEK
                     NQFVMDIEIASHRKQKNFDIIKFKGMYHINDIEKYKGFTLKVAEDQLSDLKDGEFYYH
                     EIIGLDVYEGEELIGKIKEILQPGANDVWVVERHGKRDLLLPYIPPVVLEVDLSNQRV
                     QVELMEGLDDED"
     gene            936..1691
                     /gene="trmD"
                     /locus_tag="VBC28_001468"
     CDS             936..1691
                     /gene="trmD"
                     /locus_tag="VBC28_001468"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEO6815444.1"
                     /translation="MMKIDILTLFPEMFAPLEHSIVGKAKERGLLEINYHNFRENAEK
                     SRHVDDEPYGGGQGMLLRAQPIFDTIDKIDAQKARVILLDPAGRTFDQDFAEELSKED
                     ELIFICGHYEGYDERIKSLVTDEVSLGDFVLTGGELAAMTMVDATVRLIPEVIGKETS
                     HQDDSFSSGLLEYPQYTRPYDYLGMTVPDVLMSGHHENIRKWRLEQSLRKTLERRPDL
                     LENYAMTDEERLILEKIKTEIERTDTVNEQNNL"
     gene            1672..2664
                     /locus_tag="VBC28_001469"
     CDS             1672..2664
                     /locus_tag="VBC28_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEO6815445.1"
                     /translation="MNKTIYDITIVGGGPVGLFAAFYAGLRGVSVKIIESLSELGGQP
                     AILYPEKKIYDIPGYPVITGRELIDKHIEQLERFKDSIEICLKEEVLSFEKVDDVFTI
                     QTDKDQHLSRAIVFACGNGAFAPRLLGLENEENYADNNLFYNVTKLEQFAGKHVVICG
                     GGDSAVDWANELDKIAASVAIVHRRDAFRAHEHSVDILKASGVRILTPYVPIGLNGDS
                     QRVSSLVVQKVKGDEVIELPLDNLIVSFGFSTSNKNLRYWNLDYKRSSINVSSLFETT
                     QEGVYAIGDAANYPGKVELIATGYGEAPVAINQAINYIYPDRDNRVVHSTSLIK"
     gene            2801..3871
                     /locus_tag="VBC28_001470"
     CDS             2801..3871
                     /locus_tag="VBC28_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HEO6815446.1"
                     /translation="MTNTVTPKETAGSFINKVLGGTATAIVVALIPNAILATFLKPFL
                     SYGLAAEFLHIVQVFQFFTPIMAGFLIGQQFKFTPMQQLAVGGAAYIGSGAWAYTEVI
                     QKGVATGSFQLRGIGDLINMMLTAALAVLAVKWFGNKFGSLTIILLPIIIGTGVGYLG
                     WKLLPYVSYVTTLIGQGINSFTTLQPIAMSILIAMAFSMLIVSPISTVAIGLAIGLNG
                     MSASAASMGVASTTAVLVWATMKANKSGVPIAIALGAMKMMMPNFLKHPVMAIPMLMT
                     ATVSSLTVPLFKLVGTPASSGFGLVGAVGPIASFEAGASMLIVILSWLVIPFAVGFVS
                     HKICKDILKLYKDDIFVFEGQN"
     gene            3884..4807
                     /locus_tag="VBC28_001471"
     CDS             3884..4807
                     /locus_tag="VBC28_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000964166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEO6815447.1"
                     /translation="MLVYIAGSGAMGCRFGYQISKTNHDVILLDNWADHIMAIKENGL
                     KVTGDTEDLVKLPIMKPTDATEEADLIILFTKAMQLPNMLQDIKKIIGKKTKVLCLLN
                     GLGHEDVIRQYIPEHNILMGVTVWTAGLKGPGHAHLEGVGSVNLQSIDPNNQEAGHRV
                     TELLNEAKLQATYDENVLPNIWRKACVNGTMNSTCALLDCTIGQLFASEDGVNMVHEI
                     IHEFVTVGKAEGVELDEEEITKYVMDTSVKAAHHYPSMHQDLVQNQRLTEIDFLNGAV
                     NKKGENLGIDTPYCRLITQLIHTKENVLSIK"
     gene            5019..6653
                     /locus_tag="VBC28_001472"
     CDS             5019..6653
                     /locus_tag="VBC28_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEO6815448.1"
                     /translation="MMKKGQVNDTKQSYSLRKYKFGLASVILGSFIMVTSPVFADQTT
                     SVQVNNQTGTSVDANNSSNETSASSVITSNNDSVQASDKVVNSQNTATKDITTPLVET
                     KPMVEKTLPEQGNYVYSKETEVKNTPSKSAPVAFYAKKGDKVFYDQVFNKDNVKWISY
                     KSFCGVRRYAAIESLDPSGGSETKAPTPVTNSGSNNQEKIATQGNYTFSHKVEVKNEA
                     KVASPTQFTLDKGDRIFYDQILTIEGNQWLSYKSFNGVRRFVLLGKASSVEKTEDKEK
                     VSPQPQARITKTGRLTISNETTTGFDILITNIKDDNGIAAVKVPVWTEQGGQDDIKWY
                     TAVTTGDGNYKVAVSFADHKNEKGLYNIHLYYQEASGTLVGVTGTKVTVAGTNSSQEP
                     IENGLAKTGVYNIIGSTEVKNEAKISSQTQFTLEKGDKINYDQVLTADGYQWISYKSY
                     SGVRRYIPVKKLTTSSEKAKDEATKPTSYPNLPKTGTYTFTKTVDVKSQPKVSSPVEF
                     NFQKGEKIHYDQVLVVDGHQWISYKSYSGIRRYIEI"
     gene            6797..8032
                     /locus_tag="VBC28_001473"
     CDS             6797..8032
                     /locus_tag="VBC28_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyltransferase"
                     /protein_id="HEO6815449.1"
                     /translation="MTLRELTIEEFKEHSGNYDSQSFLQTPEMAKLLEKRGYDVRYLG
                     YQVENKLEIISLSYIMPVTGGFQMKIDSGPVHSNSKYLKQFYKALQGYAKSNGVLELI
                     VEPYDDYQLFTSSGVPSNQGNDNLIEDFTSSGYHHDGLTTGFTGKYLSWHYVKNLEGV
                     TSETLLSSFSKTGRALVKKAMSFGIKVRVLKRDELHLFKEITTSTSNRRDYMDKSLDY
                     YQDFYDSFEGKAEFVIATLNFREYDHNLQIKAEALENKLKLLDERFRENADSPKYHRQ
                     RSEIINQLASFETRRQEVQSFIQKYDNQDVVLAGSLFVYSLKETVYFFSGSYTEFNKF
                     YAPAVLQEYVMQEALKRGSTFYNLLGIQGTFDGSDSILRFKQNFNGCIIRKMGTFNYY
                     PSPFKYKGIQLLKKVLKRN"
     gene            8097..8906
                     /locus_tag="VBC28_001474"
     CDS             8097..8906
                     /locus_tag="VBC28_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEO6815450.1"
                     /translation="MLDIERKRDIIKLQTISIQGGTMLKSKRKEIILSRLEQNKSVTL
                     DELTSILETSESTVRRDLDELESEGFLKRVHGGAELPYSLGQELSNQEKAIKNVQKKL
                     DIARQTAKLIAKQDVIFIDAGTTTELLIDFLPHEQLTVVTNSIHHAAKLVDRGIKTII
                     IGGAVKHSTDASIGQVAINQIRQITVDKAFLGMNGIDEVYLTTPDLEEAAIKEAIINN
                     SQQTFILMDSSKIGQVTFAKVKEINDINLVTNKTDSELMTIIKEKMKVIQV"
     gene            8903..9814
                     /gene="pfkB"
                     /locus_tag="VBC28_001475"
     CDS             8903..9814
                     /gene="pfkB"
                     /locus_tag="VBC28_001475"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017284863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HEO6815451.1"
                     /translation="MIYTVTLNPSIDFIVRLDTLLLGSVNRMTSDDKYVGGKGINVSR
                     ILKRLKIDNTATGFIGGFTGHFVEDGLVSEGIKTDFVSVNEDTRINVKVKAKIETEIN
                     GGGPRITNEQLHRLEKLLSRLTPEDTVVFAGSAPASLGNKVYNTLIPIAKKTGAEVVC
                     DFEGQTLLDALAYQPLLVKPNNHELADIFGVELEGLPDIEKYAHKILDKGAKNVIVSM
                     AGDGALLVTPEASYFAKPIKGEVKNSVGAGDSMVAGFTGEFVKSKNPVEALKWGVACG
                     TATTFSDDLATAEFIQDIYNKVEVEKL"
     gene            9811..11775
                     /locus_tag="VBC28_001476"
     CDS             9811..11775
                     /locus_tag="VBC28_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEO6815452.1"
                     /translation="MKIQDLLKKEVMIMDLKATSKEAAIDEMITKLVDTGVVTNFAIF
                     KDGIMKREAQTSTGLGDGIAMPHSKNAAVKEATVLFAKSASGVDYEALDGQPTDLFFM
                     IAAPDGANDTHLAALAELSKYLLKEGFADQLRQAKTPDDIIATFDSNSISQETVAPQT
                     VQSTSKGSDYIVAVTACTTGIAHTYMAEEALKKKAAEMGVGIKVETNGASGVGNKLTS
                     SDIARAKGVIIAADKAVEMDRFDGKPLVSRPVADGIKKSEDLINIILDNKAQTYHAKN
                     QNDKQSGESDGKSGLGSAFYKHLMGGVSQMLPFVIGGGIMIAIAFLFDNILGVPKDQL
                     SNLGSYHEIAALFKNIGGAAFAFMLPVLAGYIAYSIAEKPGLVAGFVAGSIASSGLAF
                     GKVPFAEGGKATLALAGVPSGFLGALVGGFLAGGVILLLRKLLSGLPKSLEGIKSILL
                     YPLLGVLITGFLMLLVNIPMAAINTALNTFLQGLSGSSAVLMGLLVGGMMAVDMGGPV
                     NKAAYVFGTGTLAATVANGGSVVMAAVMAGGMVPPLAVFVATLLFKDKFNNEERQSGL
                     TNIVMGLSFITEGAIPFGAADPARAIPSFIVGSALTGALVGLAGIKLMAPHGGIFVIA
                     LTSNPLLYILFILIGAVVSGVLFGLFRKIK"
     gene            11964..>12438
                     /locus_tag="VBC28_001477"
     CDS             11964..>12438
                     /locus_tag="VBC28_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2127 domain-containing protein"
                     /protein_id="HEO6815453.1"
                     /translation="MIKWNEKETKLVDTSFSIMLYLKTLFAVGQVVAGVLLYYFKPTQ
                     IKHLLFLLTEGEVHGGIHGFISAHFLKISSYINDSASHLAAIYLLIHGGIKLVALILL
                     HKEKLFAYPLSILVFVGFIIFQLREYFSSHSILMIFLCVFDVIMIILTWLEYQKMK"
BASE COUNT         4147 a         1835 c         2521 g         3935 t
ORIGIN      
        1 tgggatgaat aataagcagt accatgaact acttttacaa gctattatcg aaaaaaggga
       61 ccaaaaagtt tttgaatata tttcaaaaat gaaacataaa atactttgat agactctata
      121 tgagctttat taaaagggat aaaatcgaaa tttaaaattt aattcgaaaa gatattgaca
      181 gtaatctaga atttcgatat actagatttt gtcttttggg gtatagccaa gcggtaaggc
      241 aagggacttt gactccctca tgcgttggtt cgaatccagc taccccagtt tcacatggtc
      301 cgttggtcaa ggggttaaga caccgccttt tcacggcggt aacacgggtt cgaatcccgt
      361 acggactatc gtggccagtt gcattttagc ggctggtttt tcttttattt tcaaaaatat
      421 cggagttact atggaatact ttaacgttgg aaaaattgtc aatacacaag gattacaagg
      481 cgaaatgcgt gtattaagcg tgactgactt tgtagaagaa cgctttaaaa aaggtcaagt
      541 gttagcttta ttcgatgaaa agaatcagtt tgttatggat attgaaattg ctagtcatcg
      601 taagcagaag aattttgaca ttataaaatt caaaggaatg tatcatatta atgatattga
      661 aaaatataaa ggtttcacac tgaaggtggc agaggatcaa ctgagcgacc ttaaagatgg
      721 tgaattttat tatcatgaaa ttattggctt agatgtctat gaaggggaag agctgattgg
      781 aaaaatcaaa gaaattttac aaccaggagc aaatgacgtt tgggttgtgg aacgtcatgg
      841 aaaacgtgat cttttattac cctatatccc gccagtagta ttagaagtgg acctttctaa
      901 tcagcgtgtt caagttgaat tgatggaagg tttagatgat gaagattgat attttaactt
      961 tatttcctga aatgtttgcc cctctggaac attctatagt tggtaaggca aaagaaagag
     1021 gacttcttga aattaattat cacaatttca gagaaaatgc tgagaagtct cgacatgttg
     1081 atgatgagcc ttacggtggg gggcaaggga tgcttttacg tgctcaacct atttttgata
     1141 caattgataa aatagatgct caaaaagcac gtgttatttt gctagaccca gcgggcagaa
     1201 catttgatca ggattttgca gaagaactat ccaaagaaga tgaattaatt tttatttgtg
     1261 gtcactatga aggttatgat gaacgtataa aatcattagt aacagacgaa gtatctttgg
     1321 gagattttgt tcttacggga ggagaattgg cggctatgac aatggtcgat gctaccgttc
     1381 gtctgattcc agaagttata ggtaaagaaa caagtcatca ggatgattca ttttcatcgg
     1441 gcctattaga atatcctcag tatacgcgcc catatgatta tttaggcatg acagttcctg
     1501 atgttctcat gagtggtcat catgaaaata tccgaaaatg gcgtttagaa caaagtttgc
     1561 gaaaaacact agaacgtcga ccagacttgt tagaaaatta tgcaatgacg gatgaagaga
     1621 gacttatctt agagaaaatt aaaacagaaa tagaaagaac agatacagta aatgaacaaa
     1681 acaatttatg atatcactat tgtaggaggc ggaccagtag gtctctttgc tgctttttat
     1741 gctggactac gcggagtatc cgttaaaatt attgaaagcc tttcggaatt aggaggccag
     1801 ccagctatcc tttatcctga gaaaaaaatc tatgatattc ctggttatcc ggtgattact
     1861 ggacgtgaat taattgataa acatattgaa caattagaga gatttaaaga ttctattgaa
     1921 atctgtttaa aagaagaagt tttatcattt gaaaaagtag atgatgtttt cacaattcaa
     1981 acagataaag atcaacattt gtcacgtgct attgtttttg cttgtggcaa cggtgctttt
     2041 gctccgcgtt tactaggttt ggaaaatgaa gaaaattatg cagataataa tttattctat
     2101 aacgttacaa agttagaaca gtttgcaggt aaacatgttg ttatttgtgg tggaggcgac
     2161 tcagctgttg attgggccaa tgaattagat aagattgcag ctagtgtagc gattgttcat
     2221 cgtagagatg cttttagagc gcatgagcat agtgtggata tactaaaggc atcaggtgtt
     2281 agaattttaa ccccttatgt cccaattggt ttaaatggag atagtcagag agttagcagc
     2341 ttggtcgttc aaaaagttaa aggtgatgaa gtaattgaat tacctttaga caacttgatt
     2401 gttagttttg gcttttctac ttctaataaa aatttacgtt attggaattt ggattataaa
     2461 cggtctagta tcaatgtttc ttcacttttt gagacgactc aagaaggcgt gtatgctatt
     2521 ggagatgctg ctaattatcc aggaaaggta gaattgattg caacaggata tggggaagca
     2581 ccagtcgcta taaaccaagc tatcaattat atttatcctg atcgagataa ccgtgtagtc
     2641 cattcaacat cattgataaa ataactataa ataaaactga taagtttata aaaaaggatt
     2701 atatatattg ttaaaaaaat aacaaagctt cctatttatt ttgtgatata attaactttg
     2761 tcgttatgaa tgatattaaa taaatgaagc gaggtatttt atgacaaata cggtaacacc
     2821 aaaagagacg gctggttctt ttatcaataa agtgttaggt gggacagcaa ctgcaatcgt
     2881 tgtagcactc atcccaaatg ctatcctagc cacttttttg aaaccatttt tatcttacgg
     2941 acttgctgca gagttcttac acatcgtgca ggtattccaa tttttcacac caattatggc
     3001 aggatttttg attggtcaac agtttaaatt caccccaatg caacagcttg ctgttggagg
     3061 tgctgcatat attggttctg gtgcgtgggc ttacacagaa gttattcaaa aaggagttgc
     3121 tactggtagt ttccaattgc gtgggattgg tgatctcatc aatatgatgc taacagcagc
     3181 tttagcagtt ttagcagtta agtggtttgg taataaattt ggttccctaa ccattatctt
     3241 gttacctatt atcataggta caggcgtagg ctatttagga tggaaattac tcccttacgt
     3301 ttcatatgtg acaacactta ttggtcaagg aattaattct tttactactt tacaaccaat
     3361 tgcgatgtca attctaattg cgatggcttt ttcaatgctt attgtaagtc ctatttcaac
     3421 agtagcgatt ggtctagcga ttggtcttaa tggtatgtca gcaagtgcag cttcaatggg
     3481 agttgcttca acaacagcag tgctagtttg ggcaacgatg aaggctaata agtctggagt
     3541 tccaattgct attgctttag gtgcaatgaa gatgatgatg ccaaatttcc taaagcatcc
     3601 agtaatggcg attccaatgc ttatgactgc tacagtaagt tctctaacag tcccattgtt
     3661 taaattggtg gggacaccag cttcgtctgg ttttgggctt gttggtgcag taggtccgat
     3721 tgcttcattt gaagcaggag caagtatgtt gattgttatc ttatcttggc tcgttattcc
     3781 ttttgcagta ggttttgtat cacacaaaat ttgtaaagac attctgaaac tttataaaga
     3841 cgatattttc gtttttgaag gtcaaaatta atttggaggt tgtatgttag tttatattgc
     3901 tggttcaggc gctatgggat gtcgttttgg atatcaaatt tctaaaacaa atcatgatgt
     3961 tattttactt gataattggg cagaccatat tatggctatt aaagaaaatg gcttgaaggt
     4021 aacaggtgat acagaggacc ttgttaaact tcctatcatg aagccaactg atgcgacaga
     4081 agaagcagat ttaattattt tgtttactaa agcaatgcag ttgccaaata tgcttcaaga
     4141 tattaaaaaa attattggta agaaaacaaa ggtcttatgt cttttaaatg gattaggaca
     4201 tgaagatgtc attcgtcaat atatccctga acacaatatt ttgatgggag taacagtttg
     4261 gactgcaggg ttgaaaggac cagggcatgc tcatctggaa ggagttggtt ctgtaaatct
     4321 tcaaagtatc gatcctaaca atcaagaagc aggtcatcgc gttactgaat tgcttaatga
     4381 agcaaaatta caggctactt atgacgaaaa tgttttgcca aatatttgga gaaaagcttg
     4441 tgttaacgga acgatgaatt caacttgcgc cttgctagat tgtacaattg gtcaactctt
     4501 tgctagtgaa gacggcgtaa atatggtaca tgaaattatc catgaatttg ttactgttgg
     4561 aaaagcagaa ggtgtagagc ttgatgaaga agaaataaca aagtacgtta tggatacatc
     4621 tgttaaagca gcccatcatt acccttcaat gcatcaagac ttagttcaaa atcagcgctt
     4681 aactgaaatt gatttcttaa atggcgcagt gaataaaaaa ggtgaaaacc taggcattga
     4741 tacaccttac tgtcgattaa tcacacaatt aattcacact aaagaaaatg tacttagcat
     4801 aaaataataa tcaaccggga gaatagtgcc cggttttttg atatgcttaa tgataatgat
     4861 tttgttatac taagaataat aagagttggg tatttttttc gagaagtatt tacagcataa
     4921 aatattacat ttgtattaca gatatttcta aaatatgttg acatgttaca aaaaggatat
     4981 tataatagta gtgtaaataa ataaggaagt aggtgtttat gatgaaaaaa ggacaagtaa
     5041 atgatactaa gcaatcttac tctctacgta aatataaatt tggtttagca tcagtaattt
     5101 tagggtcatt cataatggtc acaagtcctg tttttgcgga tcaaactaca tcggttcaag
     5161 ttaataatca gacaggcact agtgtggatg ctaataattc ttccaatgag acaagtgcgt
     5221 caagtgtgat tacttccaat aatgatagtg ttcaagcgtc tgataaagtt gtaaatagtc
     5281 aaaatacggc aacaaaggac attactactc ctttagtaga gacaaagcca atggtggaaa
     5341 aaacattacc tgaacaaggg aattatgttt atagcaaaga aaccgaggtg aaaaatacac
     5401 cttcaaaatc agccccagta gctttctatg caaagaaagg tgataaagtt ttctatgacc
     5461 aagtatttaa taaagataat gtgaaatgga tttcatataa gtctttttgt ggcgtacgtc
     5521 gatacgcagc tattgagtca ctagatccat caggaggttc agagactaaa gcacctactc
     5581 ctgtaacaaa ttcaggaagc aataatcaag agaaaatagc aacgcaagga aattatacat
     5641 tttcacataa agtagaagta aaaaatgaag ctaaggtagc gagtccaact caatttacat
     5701 tggacaaagg agacagaatt ttttacgacc aaatactaac tattgaagga aatcagtggt
     5761 tatcttataa atcattcaat ggtgttcgtc gttttgtttt gctaggtaaa gcatcttcag
     5821 tagaaaaaac tgaagataaa gaaaaagtgt ctcctcaacc acaagcccgt attactaaaa
     5881 ctggtagact gactatttct aacgaaacaa ctacaggttt tgatatttta attacgaata
     5941 ttaaagatga taacggtatc gctgctgtta aggtaccggt ttggactgaa caaggagggc
     6001 aagatgatat taaatggtat acagctgtaa ctactgggga tggcaactac aaagtagctg
     6061 tatcatttgc tgaccataag aatgagaagg gtctttataa tattcattta tactaccaag
     6121 aagctagtgg gacacttgta ggtgtaacag gaactaaagt gacagtagct ggaactaatt
     6181 cttctcaaga acctattgaa aatggtttag caaagactgg tgtttataat attatcggaa
     6241 gtactgaagt aaaaaatgaa gctaaaatat caagtcagac ccaatttact ttagaaaaag
     6301 gtgacaaaat aaattatgat caagtattga cagcagatgg ttaccagtgg atttcttaca
     6361 aatcttatag tggtgttcgt cgctatattc ctgtgaaaaa gctaactaca agtagtgaaa
     6421 aagcgaaaga tgaggcgact aaaccgacta gttatcccaa cttacctaaa acaggtacct
     6481 atacatttac taaaactgta gatgtgaaaa gtcaacctaa agtatcaagt ccagtggaat
     6541 ttaattttca aaagggtgaa aaaatacatt atgatcaagt gttagtagta gatggtcatc
     6601 agtggatttc atacaagagt tattccggta ttcgtcgcta tattgaaatt taaagctaaa
     6661 tctttttaga tttagcttta ctaatattat gtgtgttgga aaaaacagtg gagatttacg
     6721 tcaaaattat attatttgga tagtatcgaa tagatagaat gtggagaaaa gtaggaaata
     6781 gttaagaaag gtatttatga cgcttcgaga attaacaata gaagaattta aagaacattc
     6841 aggaaattat gattcacaat catttttaca aacacctgag atggctaaac ttttagaaaa
     6901 acgcggctat gatgttaggt atttgggata tcaagtagaa aataaactag agataatcag
     6961 tttatcttat attatgccag tcactggtgg ttttcaaatg aaaattgatt caggaccagt
     7021 tcattcaaat tctaagtatc taaaacaatt ttataaagca ttgcaaggct atgccaaatc
     7081 caacggtgtt ctagaattaa tagttgagcc ttatgatgat taccaattat tcactagttc
     7141 gggagttcct agtaatcagg gaaatgataa tctgattgaa gattttacca gttcaggtta
     7201 tcaccatgat ggtttaacaa ctggttttac tggtaaatat ttatcttggc actatgttaa
     7261 aaatttagaa ggtgtcactt ctgaaacgtt actatcttca ttctctaaga caggacgagc
     7321 tttggttaag aaagcaatgt cttttggaat caaggttcgc gttcttaaac gtgatgagct
     7381 acatttattt aaagagataa caacttctac gtcaaataga cgtgattata tggataagtc
     7441 cttagattat tatcaagatt tttacgatag ctttgaaggc aaggctgaat ttgtgattgc
     7501 cactttaaat tttagagaat acgaccataa cttgcaaata aaagctgaag cattggaaaa
     7561 taagcttaaa ttgcttgatg aacgttttag ggaaaatgca gactctccta agtatcatag
     7621 gcaacgctct gaaataatta atcaactggc tagttttgaa acacgacgac aagaagttca
     7681 gtcatttatt caaaagtatg ataaccaaga cgttgtttta gctggtagtc tatttgttta
     7741 tagtcttaag gagacagttt atttcttttc aggatcctac acagaattta ataaatttta
     7801 tgcaccagca gttcttcagg aatatgttat gcaagaggct ctcaaaagag gttctacttt
     7861 ctataacctc ctaggtattc agggaacttt tgatggttca gatagtattc ttcgctttaa
     7921 acagaatttt aatggttgta tcattcgtaa aatgggaact tttaactatt atccaagtcc
     7981 ttttaaatac aaaggaattc agcttcttaa aaaggttttg aagagaaatt aaagtatttg
     8041 taaaagctaa tctttagatt aggctttttt agtttattga tagtttttgg aagaaaatgc
     8101 ttgacattga aagaaaaaga gatataatta aattgcaaac gatttcaatt caaggaggga
     8161 ctatgctaaa gtcaaagaga aaagaaatta tcttatcacg acttgagcaa aataagagtg
     8221 ttactttaga tgaattaacg tctatcttag agacttctga atctacagtt cgcagggatt
     8281 tagatgaact tgaatctgaa ggttttttaa aaagggtgca tggtggtgct gaattaccat
     8341 actcacttgg ccaggaatta agtaatcaag aaaaagctat caaaaacgtt caaaaaaaat
     8401 tggatattgc tagacaaact gcaaagctga tagcaaagca agatgttata tttattgatg
     8461 ccggaactac gacagaatta ttaattgatt ttctccctca cgagcaatta acggtggtga
     8521 cgaattctat tcatcatgct gcaaagttag ttgatagagg gataaaaaca atcattatcg
     8581 gaggagcggt taaacattca acagatgcta gtattggcca ggtagccatt aatcagatta
     8641 ggcagataac ggttgacaaa gcttttctgg ggatgaatgg tatagatgaa gtttatttaa
     8701 ctacaccaga tttagaagaa gctgctatta aagaagctat tattaataat agtcaacaaa
     8761 cctttattct aatggattca tctaaaattg gacaagttac atttgctaaa gtgaaagaga
     8821 taaatgacat taatttagta actaataaga ctgattcaga acttatgaca ataataaaag
     8881 agaaaatgaa ggtgattcaa gtatgattta tacagtaaca ttaaatccat cgattgattt
     8941 tattgttcgt ctagatacac tattgttggg tagtgttaac cgtatgacta gcgatgataa
     9001 atatgtaggt ggtaaaggaa ttaatgttag tcgcatatta aaacgcttaa aaattgacaa
     9061 tactgctaca ggttttatcg gtggatttac aggacatttt gttgaagatg gacttgtatc
     9121 agaaggtatc aaaactgact ttgtttctgt taatgaagat acacgtatca acgttaaagt
     9181 aaaagcaaaa attgaaactg aaatcaatgg cgggggtcct aggataacaa atgaacaact
     9241 tcatcgtcta gagaaacttc tttcgagact aacacctgag gacacagtag ttttcgcagg
     9301 ttcagcacca gctagccttg gaaataaggt ctataataca ttgattccaa ttgctaaaaa
     9361 gacaggagcg gaagttgttt gcgattttga aggtcagaca cttttagacg ctcttgctta
     9421 tcagcctctt ttagtaaaac cgaataacca tgaattagca gatatttttg gcgtagagct
     9481 agaaggtctg cctgatatcg aaaaatatgc acataagatt ctagataaag gagctaagaa
     9541 tgttatcgtt tctatggcag gggatggtgc attgttagta actccagaag cttcatattt
     9601 tgcaaaaccg attaagggtg aagtgaaaaa ttccgttggg gctggtgatt caatggtagc
     9661 tggctttaca ggagaatttg ttaagtcaaa aaatccagta gaagcactaa aatggggcgt
     9721 agcttgtggt acagcaacca ctttctcaga tgatttagca acagctgagt ttatccaaga
     9781 tatttataat aaagttgagg tagaaaaatt atgaaaatcc aagacctact aaaaaaagaa
     9841 gttatgatta tggatttaaa agcaaccagt aaagaggctg caattgatga aatgattaca
     9901 aaattagttg acacaggtgt tgtgacaaac tttgctattt ttaaagatgg tatcatgaag
     9961 cgtgaggcac aaacatcaac tggattaggt gatggtatcg ctatgccaca tagtaaaaat
    10021 gctgccgtta aagaagctac tgtccttttt gcaaaatctg catcaggtgt tgattacgaa
    10081 gcacttgatg gacagccaac tgatttattc tttatgattg ctgcgccaga tggtgctaat
    10141 gatacgcatt tagctgcctt ggcagaacta tctaaatatc ttttaaaaga agggttcgct
    10201 gatcaattac gtcaagcaaa aacgccagat gacattattg ctacttttga tagtaatagc
    10261 atttctcaag aaacagtagc tccacaaaca gtacaatcaa caagtaaagg ttcagattat
    10321 attgttgcag taacagcttg cactacggga attgcccaca cgtatatggc agaagaagct
    10381 ttaaagaaaa aagcagctga aatgggtgta ggtattaaag ttgaaacaaa tggcgcttct
    10441 ggtgtaggaa ataagttgac ttcaagtgat attgcaaggg ctaagggtgt tattattgca
    10501 gcagataagg ctgtagagat ggatcgtttt gatggcaaac ctttggtatc acgtccggta
    10561 gctgatggta ttaaaaaaag tgaagactta attaacatta ttctcgataa taaggcacaa
    10621 acctatcatg caaaaaatca aaatgataaa caatcaggtg aatctgatgg aaaatcaggc
    10681 ttaggctctg ctttttataa acaccttatg ggtggtgtat cacaaatgtt accattcgtt
    10741 attggtggtg gtattatgat tgcgattgct ttcctttttg ataacatact tggtgtacca
    10801 aaagaccaat taagtaattt aggttcatac catgaaattg cagccttatt caaaaatatc
    10861 ggtggcgcag catttgcatt tatgttacca gtattggcag gttatatcgc ttattcaata
    10921 gctgaaaaac caggactagt agctggcttc gtagctggtt caattgcatc aagtggactg
    10981 gcattcggta aagtaccttt tgcagaaggt gggaaagcaa ccttagcatt agcaggtgtg
    11041 ccatcaggtt tcttaggggc tttagtaggt ggtttcttag ctggtggagt tatcctacta
    11101 cttagaaaat tactttcagg tctcccaaaa tcgcttgaag gtattaaatc aattcttcta
    11161 tacccattgt taggtgtttt gattacaggt ttcttgatgt tgctcgtaaa cattccaatg
    11221 gcagctatca acacagctct taacactttc ttacaaggtt tatctggtag ttcagcagtc
    11281 ttgatgggct tactagttgg tggtatgatg gctgtagata tgggtgggcc tgttaataaa
    11341 gcagcttatg tttttggtac aggtactctt gcagcaactg ttgcaaatgg cggctctgtt
    11401 gttatggcag ctgtaatggc tggaggaatg gttccaccgt tagcagtatt tgtggcaact
    11461 ttattattta aagataaatt taataatgaa gaacgtcaat ctggtctaac aaatatcgtt
    11521 atggggttat cttttatcac tgaaggtgct attccgtttg gtgctgctga cccagctcgt
    11581 gcaatcccta gctttattgt aggttcagcc ttaactggag cacttgttgg tttagctggt
    11641 atcaagttga tggctcctca tggagggatt tttgtcatcg cattaacaag taatccttta
    11701 ctttatattc tatttattct aattggagcg gtagtctcag gtgtactctt tggcttgttc
    11761 cgtaaaataa aatagttaat ctcaacttat ccaaatttac attgaataaa aggtgaataa
    11821 actatcgtta ttgggtctat tcgccttttt atgttgtaaa agaatggtag attaatcatt
    11881 aataattgct aatctttttt caataggata tgatttattt aagcaaaaaa atggataata
    11941 tgattgagga aaggaggtaa gcaatgatta aatggaatga aaaagaaaca aaactagttg
    12001 atacaagttt ttcaattatg ttgtatctaa aaacgctatt tgcagtcgga caagtagtag
    12061 ctggcgtttt attatattat tttaaaccta cacaaataaa acatctccta tttcttttaa
    12121 ctgaaggtga agttcacggt gggattcatg gttttatttc tgctcatttc ttgaaaataa
    12181 gcagttatat taatgattcg gctagtcatt tagccgctat ctatctatta attcatggcg
    12241 gtatcaaatt agtagcactt attttattac ataaagagaa attatttgct taccctttat
    12301 caattttagt tttcgttggg tttatcattt tccaactaag ggaatatttt tctagtcata
    12361 gcattttaat gatttttcta tgtgtatttg atgttatcat gataatttta acatggttag
    12421 aatatcagaa aatgaaat
//
LOCUS       DATRMA010000056        12361 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.056, whole genome shotgun sequence.
ACCESSION   DATRMA010000056 DATRMA010000000
VERSION     DATRMA010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12361
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.056"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            56..2293
                     /gene="metE"
                     /locus_tag="VBC28_001478"
     CDS             56..2293
                     /gene="metE"
                     /locus_tag="VBC28_001478"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019806054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEO6815454.1"
                     /translation="MVKVSNLGYPRLGEQREWKQAIEAFWAGNLEQKDLEKQLKQLRI
                     NHLKKQKEAGIDLIPVGDFSCYDHVLDLSFQFNVIPKRFDEYERNLDLYFAIARGDKD
                     NVASSMKKWFNTNYHYIVPEWEVETKPHLQNNYLLDLYLEAREVVGDKAKPVITGPIT
                     YVSLSSGIVDFEATVQRLLPLYKQVFQDLIDAGATYIQIDEPIFVTDEGELLVDIAKS
                     VYDFFAREVPQAHFIFQTYFESAVCLDKLSKLPVTGFGLDFIHGRAENLAAVKQGLFR
                     EKELFAGIVNGRNIWAVNLEETLALLEEIGPFVKRLTLQPSSSLLHVPVTTKYETHLD
                     PVLKNGLSFADEKLKELELLASAFDGNKTKGYHEALSRFSALQAADFRHVALESLAEV
                     KLERSPYKLRQALQAEKLQLPILPTTTIGSFPQSPEIRKKRLAWKRGNLSDSDYKDFI
                     KTEIRRWIAIQEDLDLDVLVHGEFERVDMVEFFGQKLAGFTTTKLGWVQSYGSRAVKP
                     PIIYGDVKHIQPLSLEETVYAQSLTKKPVKGMLTGPITITNWSFERDDISRSDLFNQI
                     ALAIKDEIQLLEQSGIAIIQVDEAALREGLPLRQQKQQAYLDDAVAAFKIATSSVKDE
                     TQIHTHMCYSKFDEIIDSIRALDADVISIETSRSHGDIIESFETAVYPLGIGLGVYDI
                     HSPRIPTKEEIIVNIQRSLKCLSKEQFWVNPDCGLKTRREAETIAALEVLVSATKEVR
                     QQLDN"
     gene            2338..4182
                     /locus_tag="VBC28_001479"
     CDS             2338..4182
                     /locus_tag="VBC28_001479"
                     /EC_number="1.5.1.20"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional homocysteine
                     S-methyltransferase/methylenetetrahydrofolate reductase"
                     /protein_id="HEO6815455.1"
                     /translation="MSKFLEKLKTDILVADGAMGTLLYTYGLDTCHESYNVTHPEKVL
                     AIHQAYIEAGADVIQTNTYGAQRHRLKNYGLEDQVVSINQAAVNIAHQATLGKETFIL
                     GTVGGFRSQRQCDLTLDNIVEETLEQVEALLATGQLDGLLFETYYDIEEITTVLKIVR
                     EMTDLPIITNISLHEAGVTSNGKPIVEALSQLVMLGADVIGLNCHLGPYHMIQSLKQV
                     PLFAQSYLSVYPNASQLSLDGENSQYQFSQNSEYFGKSAELLVAEGVRLIGGCCGTTP
                     DHIRAVKRSIRGLKPIERKVVTPIIPVKDFVRRIRRTDTLVDKVKKEVTIIAELDPPK
                     HLDIVQFQKAIRAIDQKGIAAITLADNSLSNTRICNLSIASLLKDEISTPFLLHIACR
                     DHNLIGLQSRLLGMELLGFNHILAITGDPTKLGDFPGATSVYDVTSFKLLSLIKQLNQ
                     GLSYSGASLRRPTDFTVAAAFNPNVKNLTRTVKLIEKKVASGADYFMTQPIFDHSVLK
                     ELADLTKTVEQPFFIGIMPITSYNNAVFLHNEVPGIKLSESFLSALEKVKDDKEACLT
                     LALNESKSLIDEALNYFNGIYLITPFLRYDLTLELIDYIQKKQVRKSS"
     gene            4529..5236
                     /locus_tag="VBC28_001480"
     CDS             4529..5236
                     /locus_tag="VBC28_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HEO6815456.1"
                     /translation="MVCVDFIDQKVYSEAEGLFVSDLDLGIKGLIRARYPFALDSDFI
                     SYGNLSHYCMNYLDEIVQRANQKNEKIKYNVTTLMKEEEKPFNVEERLNKQATIGQRI
                     ADDVARFGGSWTFIIVFVSIMAIWMLVNIMKPFGIQFDPYPFILLNLALSTIAAIQAP
                     LIMMSQNRAADYDRLQARNDFNVNKTSELEIRLLHEKIDHMVQQDQFELLEIQKLQTE
                     MLVSLGNQLAQLKQLQK"
     gene            complement(5401..6486)
                     /locus_tag="VBC28_001481"
     CDS             complement(5401..6486)
                     /locus_tag="VBC28_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HEO6815457.1"
                     /translation="MTTNVMANYSYGEDCFKEIPSVLATYRIQSIAFVGGKRALDSSE
                     AEVRSILEEANIKVTGSFVYGTDSTQSNIDKLVANPQVQAADAILGFGGGKALDTAKM
                     VAKELGKNSFTIPTICSNCSAGTAIAVVYNDDHSFLRYGYPESPLHIFINTRIIAQAP
                     SKYFWAGIGDGISKAPEVERATLEAKTNKLPHTAVLGQAVALSSKEAFYQFGEQGLKD
                     VEANLASRAVEEIALDILISTGYASNLVNQPDFYYNSCHAHAFYYGTTAIQRQGEFLH
                     GVVVAFGVLVLHAYFNELEELEKVARFNKSLGLPTTLADVSLSEKDIPKIVEIAMTTN
                     EYKNTPFDPKMFAQAILAADAFGQTLA"
     gene            complement(6687..7226)
                     /locus_tag="VBC28_001482"
     CDS             complement(6687..7226)
                     /locus_tag="VBC28_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topology modulation protein"
                     /protein_id="HEO6815458.1"
                     /translation="MKIAIIGYSGSGKSTLARKLGNYYNCNVLHLDSIHFAPNWEERK
                     YDDMIDDVSNMLEKRTWIIEGNYKKLLYQERLADADEIIFFDFNRFNCLWRAFKRYCK
                     FRGKTRPDMANGCPEKLDFEFISWILKDGRSDKQKSNYKQVVEDYPQKIKILKHQRDL
                     DQYLKELPVASNTDPLKSL"
     gene            7995..8762
                     /gene="cfb"
                     /locus_tag="VBC28_001483"
     CDS             7995..8762
                     /gene="cfb"
                     /locus_tag="VBC28_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAMP factor pore-forming toxin Cfb"
                     /protein_id="HEO6815459.1"
                     /translation="MNVKHMMYLSGTLVAGALLFSPAVLEVHADQVTTPQVVNHVNSN
                     NQAQQMAQKLDQDSIQLRNIKDNVQGTDYEKTVNEAITSVEKLKTSLRANPETVYDLN
                     SIGSRVEALTDVIEAITFSTQHLANKVSQANIDMGFGITKLVIRILDPFASVDSIKAQ
                     VNDVKALEQKVLTYPDLKPTDRATIYTKSKLDKEIWNTRFTRDKKVLNVKEFKVYNTL
                     NKAITHAVGVQLNPNVTVQQVDQEIVTLQAALQTALK"
     gene            8935..9237
                     /locus_tag="VBC28_001484"
     CDS             8935..9237
                     /locus_tag="VBC28_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEO6815460.1"
                     /translation="MTKKITVVALETLIAQHNNIHLIDVREEHEYRGGHISGAINLPL
                     SQLSHKFEQLDKNKEYYLVCQRGGRSIRACQFLELKGYKVINVDGGTEAWRGNLEV"
     gene            complement(9394..10257)
                     /locus_tag="VBC28_001485"
     CDS             complement(9394..10257)
                     /locus_tag="VBC28_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEO6815461.1"
                     /translation="MNQEKKRLNLIGLILLAQAIVLSLATTLFAEILQNDVWIGIAST
                     LIALLIPCFFMGFKNLKKHLTATHHTITFTTILFLLSIIYTTNNIVSTIWDVVLNHLN
                     IMESESQNYDATFNILMISYSVVVGPFFEEVLYRGIVLNLLSKYGKWFAIITSGILFG
                     LMHQDISQLLTTSIAGIIMGFIAYHYSFKVALLLHICNNFIVEIFTQLSTVNELYGTY
                     FENILLILAILFILYYLFTHRNTARRRISLHSNVREADSINSKQHTKLLLTSWPFILL
                     VIYDIVLTTIN"
     gene            complement(10562..11122)
                     /locus_tag="VBC28_001486"
     CDS             complement(10562..11122)
                     /locus_tag="VBC28_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001223122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEO6815462.1"
                     /translation="MRITIFCGASTGENPVYSEKTVALAQWMAQNKHSLVYGGGKVGL
                     MGVMADTVIANGGYTTGVIPTFLRDREIAHENLSELIIVNNMPERKAKMMLLGDAFIA
                     LPGGPGTLEEISEVISWSRIGQNDNPCILYNVNGYFNDLKNMFDHMVGEGFLSLEDRE
                     NVLFSDDITEIEDFITNYKVPSTRQY"
     gene            complement(11139..11435)
                     /locus_tag="VBC28_001487"
     CDS             complement(11139..11435)
                     /locus_tag="VBC28_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HEO6815463.1"
                     /translation="MDITDYQKWVSEFYKKRNWYQYNSFIRSNFLSEEVGELAQAIRK
                     YEIGRDRPDETEQTDLENLNDIKEELGDVLDNIFILADQYNISLEEIISAHRTN"
     gene            11649..12263
                     /locus_tag="VBC28_001488"
     CDS             11649..12263
                     /locus_tag="VBC28_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEO6815464.1"
                     /translation="MNKKSFFQCVILLVSFLVLTLAVKSQSDMISYLDNITSAFFQSI
                     RNPDLTNLMTIISTVVSPLTTSLIALVILGYQYFLNQRIAVWLFMLFFGTNALALLLK
                     DIIARHRPMNQLVFDSGYSFPSGHTISTFLLMILVLVVARQRLRRVLSQVVFVIFALV
                     ILASVIFSRLYLENHFLTDILGSLLLGASSYYGLSAIVSLKELQ"
BASE COUNT         4082 a         1932 c         2281 g         4066 t
ORIGIN      
        1 ataattcata aaactaaaag ggttagttat tagctataac ttgatggagg aaattatggt
       61 aaaagtttca aatttagggt atccacgtct tggtgaacag cgcgaatgga agcaagcgat
      121 cgaagctttc tgggcaggga atcttgaaca aaaagattta gaaaaacaac taaaacaatt
      181 acgtatcaat catttaaaga aacaaaaaga ggcaggtatt gaccttattc cagtggggga
      241 tttttcttgt tatgatcatg ttttggattt gtcatttcaa ttcaatgtaa tcccaaagcg
      301 tttcgatgag tatgagagga atttagacct ttattttgct attgcaagag gtgataaaga
      361 taatgtcgca tcatctatga aaaagtggtt taataccaac taccactaca tagtcccaga
      421 atgggaggtt gagactaaac ctcacttgca gaataattac ttacttgatc tttatctaga
      481 agctagggaa gtagttggtg ataaagcaaa gccggttatc actggtccaa taacctatgt
      541 ttccttatca tcaggaattg tcgactttga agcgactgtt cagcggttat taccacttta
      601 taagcaggtc tttcaagatc tgatagatgc aggcgccacc tatattcaga ttgatgagcc
      661 gatatttgta actgatgaag gtgaactttt agtagatata gctaagtctg tttatgattt
      721 ttttgcaaga gaagtaccac aagcccactt catctttcaa acctactttg aatcagcagt
      781 ctgtttagat aaactctcta agctgccagt aacgggattt ggccttgatt ttatacatgg
      841 tagggcggaa aatttagctg ctgttaagca aggtctattc cgcgaaaaag aattatttgc
      901 aggaattgtt aatggtcgaa atatctgggc agtaaattta gaagaaacgt tggctttatt
      961 ggaagagata ggtccctttg ttaaacgatt gactcttcaa ccttcttcaa gtcttttaca
     1021 tgtaccggtg acgactaaat acgaaacaca tttagaccct gtgttaaaga atggcttatc
     1081 atttgctgat gaaaaactaa aggaattaga actattagct agtgcttttg atggtaataa
     1141 aacaaaggga tatcacgaag ctttatctcg tttttcagct cttcaagctg ctgattttcg
     1201 tcatgtagca ttggaatcat tagcagaagt aaagcttgaa cgaagtccgt ataaattacg
     1261 ccaagctttg caagctgaaa aattacagtt accgatttta ccaacaacaa ctattggatc
     1321 ctttcctcaa tcacctgaaa ttaggaagaa acgccttgct tggaaaagag gaaatctatc
     1381 tgactcagat tataaagatt tcataaaaac tgaaattaga agatggattg ctattcaaga
     1441 agatcttgat cttgatgtgt tagtacatgg cgaatttgag cgtgttgata tggttgaatt
     1501 ttttggtcaa aagttagctg gttttacgac aaccaaatta ggctgggtac agtcttatgg
     1561 ttcaagggcg gtcaaaccac ctatcattta tggtgatgtc aaacatattc aacccttaag
     1621 ccttgaagaa acggtttatg cccaaagttt gactaagaaa cctgttaaag gcatgttgac
     1681 aggtcctatt actataacga actggtcatt tgagcgagat gatattagcc gatctgatct
     1741 ttttaatcaa attgctttgg ctataaaaga tgagattcaa cttttggaac aatcaggtat
     1801 tgctattata caagtggatg aagcagccct tcgagaaggt ttacccttac gccagcaaaa
     1861 gcaacaggct tacttagatg atgctgttgc ggcctttaaa attgcaactt catctgtgaa
     1921 agatgagaca caaattcata cacatatgtg ttattcaaaa tttgacgaaa ttattgattc
     1981 tatccgtgca ctagatgcag atgttatttc tattgaaacg agtagaagtc atggggacat
     2041 cattgaaagt tttgaaacag cagtttatcc tctaggaatt ggcctgggtg tttatgatat
     2101 tcattcccct cgcataccta ctaaggaaga aattattgtc aatattcaac gatcactaaa
     2161 atgtctatca aaagagcaat tttgggtaaa ccctgattgt ggcttaaaaa cacgccgtga
     2221 agcagaaaca attgctgcct tggaggttct tgtttcagct accaaagagg ttcgtcagca
     2281 attagataat taataggtta atcagtaaag gattgccatg agggaggagt aaagaagatg
     2341 tccaagtttt tagagaaatt aaaaacagat attctagtag ctgacggtgc aatgggaact
     2401 ctcctttata cttatgggct agatacttgt catgaatcct acaatgtaac acatccagaa
     2461 aaggttctag ccattcatca agcttatatt gaagcgggtg cagatgttat tcagacgaat
     2521 acctatgggg ctcaacgtca tcgattaaag aactacggtt tggaagatca ggttgtctct
     2581 atcaatcaag cagcagttaa tatagctcat caagcgactt tgggtaaaga aacatttatt
     2641 ttaggtactg ttggaggttt tcgttctcaa agacaatgcg atttaacatt ggataatatt
     2701 gtagaagaaa ctttggaaca agttgaggct ttattggcga caggacagct agacggatta
     2761 ttgtttgaaa cttattatga tattgaggag ataacgacag ttttaaaaat tgtgagggag
     2821 atgactgatc taccaattat tactaatatt tctcttcatg aagcaggggt aacatcaaat
     2881 ggaaagccta ttgtagaggc gctcagccaa ttagttatgc taggtgcaga tgtgattggc
     2941 ttaaattgtc accttggacc ctatcatatg atccaatcat tgaagcaagt tcctttattt
     3001 gcccagtctt atttgtcagt ttatcctaat gcaagtcagt tatctttaga tggtgagaat
     3061 agtcaatacc agtttagtca gaattcagag tattttggaa aatctgcaga acttctcgta
     3121 gcagaaggag ttaggcttat tggtggttgt tgtgggacaa ctccagatca tattagagct
     3181 gtgaaacgat ctattagagg attgaaacct attgaacgaa aggttgttac tcctataata
     3241 cctgtaaaag attttgttag gcgtatcaga cgaacagata ctctagtgga taaggtaaaa
     3301 aaagaagtaa caattatcgc agaattagat cctccaaagc atttagatat cgttcagttt
     3361 caaaaggcta tacgagcgat tgaccaaaaa ggtattgcag caattacttt agcagataat
     3421 tcactatcta atacacgtat ttgtaattta agtatagcta gcttgcttaa agatgaaatt
     3481 agcacaccct ttttactaca tatagcttgt cgtgaccata atttaattgg tttacagtct
     3541 agactgttgg gcatggaatt attaggtttt aaccatattt tagccataac aggagatccg
     3601 actaaacttg gtgatttccc aggagcgact agtgtttatg atgtgacttc ctttaaactc
     3661 ctttctctga ttaaacaact taatcaaggt ttatcttata gtggtgctag cttgagacgt
     3721 ccaacagatt ttacagttgc tgctgccttt aatcctaatg tgaaaaacct tactcgaacc
     3781 gtaaaattga ttgagaaaaa agtagcttct ggtgctgatt attttatgac acaaccaata
     3841 tttgatcact ctgtgttaaa ggaattagct gatcttacta aaacagttga acaacccttt
     3901 tttatcggaa ttatgccaat aacaagttat aataacgctg tttttctaca caatgaagtt
     3961 cctggtatta aactttctga gtcattttta tctgctcttg aaaaggtaaa ggacgacaaa
     4021 gaagcttgtt taactttggc cttaaacgag agtaagtcct tgattgatga agccttgaac
     4081 tattttaatg gtatctattt gattactccc tttttacgct atgatctgac tcttgagtta
     4141 attgactata tacaaaaaaa gcaagtaaga aagtcctctt aactttctta ctttttagtt
     4201 ggtcgtttat tattataata aaagttacgt accaaaacag aggttaaact agatttttaa
     4261 gccaccttct atactaagat aaattagtta atttaagttt tctttaagtt tatagactta
     4321 gaataaagcg ttattttgat gggtagcata aaattaaggc ataatatgga tgtcagcgat
     4381 ttttttagac taacgaaata acgctgtaaa gcttaatagg ggtgtatcaa atactcactt
     4441 atatagtacc aaaagcctat agcctgttgg ctagcttgac cgtattcaaa tattgaataa
     4501 atcttggttg agaaagggag gtataaaaat ggtatgtgtc gattttattg atcaaaaagt
     4561 ttatagtgaa gcagaaggcc tatttgtttc cgatttagac ttaggcatta aaggtttaat
     4621 aagagcacgt tatccttttg ctctggatag tgactttatt tcgtatggaa atttaagtca
     4681 ttattgtatg aattatcttg acgaaattgt tcagcgagcg aatcagaaaa atgagaagat
     4741 aaaatataat gtgactacct tgatgaaaga agaagagaag cccttcaacg ttgaagagcg
     4801 tttgaacaag caagcaacta tcggtcaacg aattgctgat gatgttgcac gttttggcgg
     4861 atcttggact tttattatcg tttttgtatc cattatggct atttggatgt tggttaatat
     4921 catgaagcct tttggtattc aatttgatcc ttacccattt atcctgttaa acttagcttt
     4981 atcaacgatt gctgcaatcc aagcaccact tattatgatg agtcaaaatc gtgctgctga
     5041 ttatgatcgt ttgcaagctc gaaatgactt taatgtcaat aaaacctctg agttagaaat
     5101 tagattattg cacgaaaaga ttgatcatat ggtacaacaa gatcagtttg aattgttaga
     5161 gattcaaaag ttacagacag aaatgctagt atcattggga aatcagttag cacaattgaa
     5221 acaactacaa aaataatctt ttagcagtta tttaaaaata ctgatacaat actaagaatt
     5281 taaattaata ctgcaacttc ctataatgga gagcagtatt tttactttat taataacaaa
     5341 aaccccttag atgatgtgtc taagggattt ttgtatcgga ataatcgagt tggtgaatta
     5401 ttaggctagt gtctgtccga aagcgtctgc tgctaaaata gcttgagcaa acattttggg
     5461 atcgaaaggg gtatttttgt attcattagt agtcattgca atttcaacaa tttttggaat
     5521 atctttttct gataaagaga cgtcagctag agttgttggc aaacctagtg acttattaaa
     5581 acgagctact ttttcaagtt cttctagttc gttgaaataa gcatgtaaaa ctaacacacc
     5641 aaacgcaact acaacgccat gtaagaattc accttgtctt tgaatagcgg ttgtaccata
     5701 gtaaaatgca tgcgcgtgac aagaattgta gtagaaatca ggttgattaa ctaaattaga
     5761 agcatagcca gttgagatta agatatcaag cgcaatttct tcaactgcac gcgaagctaa
     5821 attagcttca acatctttta gaccttgttc accaaattga taaaaagctt cctttgaaga
     5881 cagagcgact gcttgtccta acactgcagt atgtggtagt ttattggtct tagcctctaa
     5941 ggtagcacgt tctacttcag gggcttttga aataccgtcc ccaataccag cccaaaaata
     6001 tttgcttggt gcctgtgcaa taatccgtgt attgataaaa atatgaagtg gagactcggg
     6061 atagccgtat cttaagaaag aatggtcatc attatataca actgcaatgg cagtacctgc
     6121 ggagcaatta gaacaaatag ttgggatagt aaagctattt ttacctaact cttttgctac
     6181 cattttagct gtgtctaaag cttttccacc gccaaaacct aagatagcat cagcggcttg
     6241 aacttgtggg ttggctacta acttgtcaat gttagattgt gttgaatccg ttccatagac
     6301 aaagcttcct gtgactttaa tatttgcttc ctctaaaata gagcgaacct cggcttctga
     6361 gctgtctagg gcacgtttgc caccgacgaa agcaatgctt tgaatacggt aagttgccaa
     6421 aacgctagga atttctttga agcaatcttc tccgtagcta taatttgcca ttacatttgt
     6481 tgtcattcag tgtcctcctc tattccaaag gtcgattaga gacagctatt ttctaaaagt
     6541 gaatagtttt ttgtctcttg accaagtgat tatcatatta tagcagaata ttcagaaaat
     6601 tggaacatga aaaacaattt aattttcaag aataagaata gaaaaggtaa agcgagatat
     6661 acgagtaaat actgtcagta taataattac aagctcttga gagggtctgt attggatgca
     6721 acagggagtt cttttaggta ttgatcaaga tcgcgttggt gttttagaat ctttattttt
     6781 tgagggtagt cctcaacaac ttgtttatag tttgattttt gtttgtcaga gcgaccgtct
     6841 ttaagaatcc aactgataaa ttcaaaatct aatttttccg ggcatccatt ggccatatca
     6901 ggtctagtct taccacgaaa cttacagtaa cgtttgaatg ctctccacaa gcagttgaag
     6961 cgattaaagt caaaaaaaat aatctcatct gcatctgcta gacgttcttg atatagcagt
     7021 tttttgtagt taccttctat aatccatgtt cttttttcta acatgttgct gacatcgtca
     7081 atcatgtcat catattttct ctcttcccaa tttggggcga agtgaatact atcaagatgt
     7141 aatacattgc aattatagta atttcccaat tttcttgcta aagtggactt acccgaccca
     7201 ctatatccga ttattgctat tttcatatac tacctccgtt aaattgttta cttgaagtta
     7261 atgctgtact taggatttac ttattttata agggttattc acttttttag attaatatat
     7321 ttttgatata agttatccat acacatggta acatagttac cagtaaatgt aatctagata
     7381 tcaatactat ggctgacgat attgttaact tttggttcaa aaaattgggg taaaaatcat
     7441 cggagggaac tgccaaacaa actcacagtt taaaatacta agtaataaaa agtcttgaac
     7501 ttattatgtt taaggctttt taagtgtcta aaatacttgg tctagtatga atttctaaac
     7561 agaaagtaaa aataatgagt tttagtatgg tttttgggca taatggaacg attttatcta
     7621 aaatagtacg cttcttatta aatttatttt ttaagaaaaa cattaggcta atctatttga
     7681 taaaaatagt gataagtaga aattttctaa aatatttaga tttatataag gtggtaaatt
     7741 atcgttatgg ttttttacat gaaaatataa attaaattat cagaaattaa caaaaggata
     7801 ggcgcaagta tattatagca aaatgataat attagtttta tgaaaaaaat gcagtttatt
     7861 tgtaataatg taaaatatag gggaaaagaa agcgcttgac gaccttttgg acaagtagta
     7921 agatacaaac atgggccctg taaattaaaa atactgcagt aaaagtgatt ttagtttaaa
     7981 ggaggaaatt cattatgaac gttaaacata tgatgtatct atctggaact ctagtggctg
     8041 gtgcattgtt attttcacca gctgtattag aagtacatgc tgatcaagtg acaactccac
     8101 aagtggtaaa tcatgtaaac agtaataatc aagcccagca aatggctcaa aagcttgatc
     8161 aagatagcat tcagttgaga aatatcaaag ataatgttca gggaacagat tatgaaaaaa
     8221 cggttaatga ggctattact agtgttgaaa aattaaagac ttcattgcgt gccaaccctg
     8281 agacagttta tgatttgaat tctattggta gtcgtgtaga agccttaaca gatgtgattg
     8341 aagcaatcac tttttcaact caacatttag caaataaggt tagtcaagca aatattgata
     8401 tgggatttgg gataactaag ctggttattc gcattttaga tccatttgct tcagttgatt
     8461 caattaaagc tcaagttaac gatgtaaagg cattagaaca aaaggtttta acttatcctg
     8521 atttaaaacc aactgataga gctaccatct atacaaaatc aaaacttgat aaggaaatct
     8581 ggaatacacg ctttactaga gataaaaaag tacttaacgt caaagaattt aaagtttaca
     8641 atactttaaa taaagcaatc acacatgctg ttggagttca gttgaatcca aatgttacgg
     8701 tacaacaagt tgatcaagag attgtaacat tacaagcagc acttcaaaca gcattaaaat
     8761 aatatttgta tttttcgtgt gatgctgtcg acttcgtgat tttgtactac catggttttt
     8821 atgattaaaa ggtttacgat aatagtcata atagtagaac gatgtcacca ttttaaataa
     8881 taaagtgatt agtcatttga ctaaatttgc caagtatcaa aggaaataaa gattatgact
     8941 aaaaagataa ctgttgtagc attagaaaca ttgattgccc agcataataa tatccatttg
     9001 atagacgttc gtgaagagca tgagtatcgt ggggggcata tttcaggtgc gataaatctt
     9061 cctttgagtc aactcagtca taagtttgaa cagttagata aaaataagga atattatctt
     9121 gtttgtcaac gagggggaag atctattaga gcatgccaat ttcttgagtt gaaaggctac
     9181 aaagtaatca atgtagatgg cggcacagaa gcgtggagag gaaatctaga agtgtaaata
     9241 tgtcctgtgt agtctaccat atttgataat aaaaaaattg acattatctc actattaatg
     9301 accttttagt aaacataaag aaaagtatgt gttttgaaaa ggaggatgat tatccttttc
     9361 aaaaccatac tttttgtgtc ttgtggcttg gtcttaatta atagttgtta ggacaatgtc
     9421 gtaaatgaca aggagaataa aaggccaact agtaagtaat aattttgtat gttgtttaga
     9481 gtttattgag tcagcctctc gaacgttaga gtgcagactt atacgacggc gagcagtatt
     9541 cctatgagta aaaaggtaat ataatataaa taagattgct aaaattagca atatattttc
     9601 aaaataagtc ccgtataact cgtttactgt tgatagctgg gtgaaaattt ctacgataaa
     9661 gttattgcag atatgaagga gtagggctac cttaaaggaa taatggtaag cgataaaacc
     9721 cattatgata cccgcaatag atgtagtcaa tagctgagaa atgtcttggt gcattaaacc
     9781 aaataggatg ccagaagtga taatggcaaa ccatttacca tacttggata ataaattcaa
     9841 tactattcct cggtataaaa cttcttcaaa aaaaggacct acaacaacag aataactaat
     9901 cataagtata ttgaatgtgg catcataatt ttgactttcc gattccataa tattgagatg
     9961 atttagcaca acatcccata ttgtggagac tatattatta gtagtatata taatgcttaa
    10021 caagaataaa atagtggtga aagtgattgt atgatgggtt gccgttagat gtttttttaa
    10081 atttttaaaa cccataaaaa aacaaggaat gagtaaggcg attaaagtac ttgctatacc
    10141 tatccagaca tcattttgta agatttcagc aaatagtgta gtcgccaaac ttaaaacaat
    10201 agcctgagct aaaagtatca gcccgattag gttaagtctc tttttttctt gattcaattg
    10261 attacctcct tattaataac ggtaacgaca aagaaatatt ataatgtata atgtataggt
    10321 ttctaagtca ttagtcaata gggacgagca cttggatact tctaataaga taaaatacat
    10381 agacaagact atagttttgg cttctctaaa gattacctaa aaatttataa gttctcgagc
    10441 agaaaggatg agtttggata ttgagaggat gggcttgacc agctgaaaga aaaatgaacc
    10501 aagggtaatc gcttcgaaaa aagaaggaaa tttcaattag aaacttcctt tttttatcaa
    10561 tctagtattg gcgcgtgcta gggactttat aattcgttat aaaatcttcg atttcagtaa
    10621 tatcatctga gaataggaca ttttctctat cttccagact aagaaaaccc tcaccaacca
    10681 tatgatcaaa catatttttt aaatcattaa agtagccatt aacattatat aaaatgcaag
    10741 gattatcatt ttgaccgatg cgagaccaag agataacttc ggaaatttct tcaagggtac
    10801 caggtccacc aggtagagcg ataaaggcat ctccaagtag catcattttt gccttacgtt
    10861 caggcatatt atttactatg ataagctctg ataaattttc atgtgcaatt tcacgatctc
    10921 ttaaaaatgt cggaataaca ccagtagtgt agccaccatt tgcaatcaca gtatcagcca
    10981 tgactcccat taagccaact tttcctccac catacaccag agaatgtttg ttttgtgcca
    11041 tccattgtgc taaggcaact gttttttcac tataaacagg attttcccca gtacttgctc
    11101 cacaaaaaat agttattctc atgtttcaaa cctcttttct aattagtgcg atgtgcgctg
    11161 ataatctctt ctagtgagat attatactga tcagcaagta tgaagatatt atctaagaca
    11221 tctcctagtt cttcttttat atcatttaag ttttccaaat cagtttgttc ggtttcgtca
    11281 ggtctgtcac gtccaatttc atatttgcga atggcttgag ccaattcgcc gacttcttct
    11341 gaaagaaagt tacttctaat aaaagaattg tattgatacc agtttctttt tttatagaat
    11401 tcactcaccc atttttgata atctgtaata tccattattc cctccgtttc aagtatattg
    11461 tagcaatatt gctttattaa tgaaaatttg aacgctaatt aataggaaaa gttcgtattt
    11521 ggtatttctt ctagtttgtg aatagtcata tttaaaccta aaaggttagc gcgtacttta
    11581 agcatttttt aaatatttta aaccataatg tatgaagatt tatctcttta ttaaagtgag
    11641 gttatgtagt gaacaagaaa tcctttttcc agtgtgttat actacttgtt tcttttttag
    11701 tgttaacatt agcagttaaa tctcaaagtg atatgataag ttatttggat aatatcactt
    11761 cagctttttt tcaaagcata cgaaatcccg atttaactaa cttaatgacg ataatatcaa
    11821 cagttgtcag ccctttgaca acgtcattaa tagcacttgt tatccttggg tatcaatatt
    11881 ttttaaacca aagaatagct gtgtggctat ttatgttatt ttttggtacc aatgctttgg
    11941 cattgctttt aaaagatatc atcgcacgtc atagacctat gaatcaactt gtttttgatt
    12001 cgggttatag ttttcccagt ggacatacga tatcaacgtt tttattaatg attcttgttt
    12061 tagtggttgc gagacagaga ttgagaagag tattgtcaca agtagttttc gtaatatttg
    12121 cattagtgat ccttgcatca gttatttttt caagactcta tttagaaaat cattttttaa
    12181 cggatatttt agggtcgttg ttactaggag catcgtcata ttatggtttg tctgctattg
    12241 tatctttgaa agagttacaa taaaaatagt agttaccaat gaattataaa agagcctttc
    12301 ttaagggctc ttttataatt cattattttt caatgacaaa tattagtgaa gttaattttt
    12361 a
//
LOCUS       DATRMA010000057        12043 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.057, whole genome shotgun sequence.
ACCESSION   DATRMA010000057 DATRMA010000000
VERSION     DATRMA010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12043
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.057"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            complement(9..1349)
                     /locus_tag="VBC28_001489"
     CDS             complement(9..1349)
                     /locus_tag="VBC28_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO6815465.1"
                     /translation="MTKKGWRIMPAIITTAILGFSGILIETSMNVTFPLLMKEFGVNP
                     AVIQWVTTGNLLAVAVTVPLSAFMIKNLSERQIFTLANVLFLSGVLIDSFAPNLAILL
                     VGRVLQGVGTGLALPLLFHIILTQIPMERRGLMMGVAAMVTLLAPAVGPTYGGVISGM
                     LGWKMIFMLLAPILIISTFIGLASIPKRQVRINDKLNFPAFVSLGIGLATLLLAIEKM
                     SIFYLLVAIVSFVIFYYLNKQLEFLNLNVFKDKDFSILLYGVLAFQMIPLALSFLLPN
                     LLQLVLHQTSTKAGLFMFPGAIAVVFLSPFAGYLLDKIGAFKPIMIGISLSLIGLIGT
                     AIFIPAKFVVVLLAFDILTKIGMGIGASNMVTTALTKLKPAQSADGNSILNTLQQFAG
                     AFATAVASQIFTIGQVAIPKNGAIIGSQFAVLFVIIVVILAIVGLTYLRKRKAI"
     gene            1641..2312
                     /locus_tag="VBC28_001490"
     CDS             1641..2312
                     /locus_tag="VBC28_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6815466.1"
                     /translation="MAFASNESENNFFITLKTYFNYLFSIQIITDISTLNHADFDGSF
                     AFHDIETSIPHLVIDSNYLAISQTNSKTEANDIKTFSELSKTMTEFHYMLNFDLFNHL
                     PYRFRLHNKDGQTIYSNHKPEDPFDIYPEEEYPIDKWVQNSLIEKKAKELHLLLPGAS
                     QDYILVQSYKRLENDSGQLVGYIEHVHNIKPLLEGYLKESGQAIVGWSDVTSGASISN
                     DDFEL"
     gene            complement(2383..4383)
                     /locus_tag="VBC28_001491"
     CDS             complement(2383..4383)
                     /locus_tag="VBC28_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001164946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HEO6815467.1"
                     /translation="MQHVNHSSFDKASKAGFIIALGIVYGDIGTSPLYTMQSLVENQG
                     GISSVTESFILGSISLIIWTLTLITTIKYVLVALKADNHHEGGIFSLYTLVRKMTPWL
                     IVPAVIGGATLLSDGALTPAVTVTSAVEGLKVVPSLQHIFQNQSNVIFATLFILLLLF
                     AIQRFGTGVIGKLFGPIMFIWFAFLGISGLLNSFAHPEVFKAINPYYGLKLLFSPENH
                     KGIFILGSIFLATTGAEALYSDLGHVGRGNIHVSWPFVKVAIILSYCGQGAWILANKN
                     AGNELNPFFASIPSQFTMHVVILATLAAIIASQALISGSFTLVSEAMRLKIFPQFRST
                     YPGDNIGQTYIPVINWFLFAITTSIVLLFKTSAHMEAAYGLAITITMLMTTILLSFFL
                     IQKGVKRGLVLLMMIFFGILEGIFFLASAVKFMHGGYVVVIIAVAIIFIMTIWYKGSK
                     IVSRYVKLLDLKDYIGQLDKLRHDHRYPIYHTNVVYLTNRMEGDMIDKSIMYSILDKR
                     PKKAQVYWFVNIKVTDEPYTADYKVDMMGTDFIVKVELYLGFKMRQTVSRYLRTIVEE
                     LLESGRLPKQGKTYSVRPDSKVGDFRFIVLDERFSSSQNLKPGERFVMLMKSSVKHWT
                     ATPIRWFGLQFSEVTTEVVPLIFTANRGLPIKEKIELTTTGD"
     gene            complement(4518..5279)
                     /locus_tag="VBC28_001492"
     CDS             complement(4518..5279)
                     /locus_tag="VBC28_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEO6815468.1"
                     /translation="MISRKVALVTGASAGFGAAIVTKLVSDGYSVIGCARRMDKLKCL
                     GEKFSEGYFYPLQMDITSRESVDKALESLPKNLQSIDLLVNNAGLALGLDKSYEADFE
                     DWMTMINTNVVGLIYLTRCILPKMVEVNRGLIINLGSTAGTIPYPGANVYGASKAFVK
                     QFSLNLRADLAGTKIRVTNLEPGLCEGTEFSTVRFKGDHKRVEKLYEGAHAIQAEDIA
                     NTVSWVASQPEHININRIEIMPVSQTYGPQPVYRY"
     gene            complement(5341..6333)
                     /gene="pta"
                     /locus_tag="VBC28_001493"
     CDS             complement(5341..6333)
                     /gene="pta"
                     /locus_tag="VBC28_001493"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEO6815469.1"
                     /translation="MRSLFGGLREKVASDYVKIVFPEGSDERVLRAAARLKFEGLVDP
                     VILGKADEVHDSLARLGFVDQDYSVIDPEQYEKFEEMKEAFVEIRKGKATMEDADRLL
                     KDVNYFGVMLVKLGLADGMVSGAIHSTADTVRPALQIIKTKPGISRTSGVFLMNREST
                     QERYIFADCAINIDPNAQELAEIAVNTADTAKIFDINPKIAMLSFSTKGSAKAPQAEK
                     VQEAAKIAKDLSPELAVDGELQFDAAFVPETAEIKAPNSDVAGKANVFIFPDLQSGNI
                     GYKIAQRLGMFEAIGPILQGLNAPVNDLSRGSSAEDIYKLAIITAAQAIDEKSK"
     gene            complement(6359..7243)
                     /locus_tag="VBC28_001494"
     CDS             complement(6359..7243)
                     /locus_tag="VBC28_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000667470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEO6815470.1"
                     /translation="MKFEYIAEERCKVKTLLKSHDVSRGLLAKIKYRGGKIFVNGEEQ
                     NAIFLLEIGDVVTIDIPDEPSHETLEPVPHDLDIVYEDDHFLILNKPFGFASIPSSIH
                     SNTIANFIKHYYVSNNYANQQVHIVTRLDRDTSGLMLFAKHGYAHARLDKQLQAKAIE
                     KRYYALVSGSGDLADSGDIIAPIARDVDSIITRRVHESGKYAHTSYQVVARYGDVRLV
                     DIKLHTGRTHQIRVHFAHIGFPLLGDDLYGGRMDLGINRQALHCHSLSFYDPFMGKIN
                     KQTLDLTDDFDSVIMELH"
     gene            complement(7240..8076)
                     /locus_tag="VBC28_001495"
     CDS             complement(7240..8076)
                     /locus_tag="VBC28_001495"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HEO6815471.1"
                     /translation="MTQMNFTDRATRVAIIANGKYQSKRVASKLFAAFKHDPDFYLSK
                     KDPDIVISIGGDGMLLSAFHMYEKQLDKVRFVGVHTGHLGFYTDYRDFEVDTLINNLK
                     NDEGEQISYPILKVTITLEDGRVIRARALNESTIKRIEKTMVADVVINQVVFERFRGD
                     GILVSTPTGSTAYNKSLGGAVLHPTIEALQLTEISSLNNRVYRTLGSSVIIPKKDAIE
                     IVPKRVGVYTISIDNKTVHYKNVTKIEYSIDEKSINFVSTPSHTSFWERVNDAFIGEP
                     EH"
     gene            complement(8051..8722)
                     /locus_tag="VBC28_001496"
     CDS             complement(8051..8722)
                     /locus_tag="VBC28_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HEO6815472.1"
                     /translation="MSMDWETFLDPYIQTVGELKIKLRGIRKQFRKQNRHSPIEFVTG
                     RVKSVESIQEKMVLRGISEENLAQDLQDIAGLRIMVQFVDDVDEVLALLRKRHDMTVV
                     QERDYITHMKSSGYRSYHVVVEYPVDTIDGQKKVLAEIQIRTLAMNFWATIEHSLNYK
                     YQGDFPEEIKQRLEKTAKIALELDEEMRKIREDIREAQLLFDPLNRKLSDGVGNSDDT
                     DEFYR"
     gene            8832..9404
                     /locus_tag="VBC28_001497"
     CDS             8832..9404
                     /locus_tag="VBC28_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HEO6815473.1"
                     /translation="MTHLEIEYKTLLNKDEFNRLTSLFSHVQPITQTNYYFDTETFEM
                     KAHRMSLRIRTLPNRAELTLKIPREVGNLEHNHDLTLEEAKYIVKNGQFPEDTEIASL
                     ILEKGVDPTKLAVFGQLTTTRREMETSIGLMALDSNIYADIKDYELELEVKQPKQGKR
                     DFDQFLKENNINFKYAKSKVARFSATLKNN"
     gene            9581..10555
                     /locus_tag="VBC28_001498"
     CDS             9581..10555
                     /locus_tag="VBC28_001498"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEO6815474.1"
                     /translation="MAEQYADKQIKLFSLTANREIAEKISQASGIPLGKMSSRQFSDG
                     EIMINIEETVRGDDIYIIQSTSFPVNDNLWELLIMIDACKRASANTVNIVVPYFGYSR
                     QDRIAASREPITAKLVANMLVKAGVDRVLTLDLHAVQVQGFFDIPVDNLFTVPLFAEH
                     YNQLGLSGEDVVVVSPKNSGIKRARSLAEYLDSPIAIIDYAQDDSEREEGYIIGEVEG
                     KKAIIIDDILNTGKTFAEAAKILERGGATEIYAVASHGLFAGGAADILESAPIREIIV
                     TDSVLSKERIPSNIKYLTASHLIADAIIRIHERKPLSPLFSYRSDKKD"
     gene            10559..11674
                     /locus_tag="VBC28_001499"
     CDS             10559..11674
                     /locus_tag="VBC28_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEO6815475.1"
                     /translation="MIYLDNAATTALTPSVIEKMTNVMTSNYGNPSSIHTFGRQANQL
                     LRECRQIIAEYLNVNSREIIFTSGGTESNNTAIKGYALANQLKGKHIITSEIEHHSVL
                     HTMTYLSERFGFDITYLKPNHGQITAKDVQEALRDDTIMVSLMFANNETGDFLPIQEI
                     GQLLRNHQAVFHVDAVQVFSKMELDPHSLGIDFLAASAHKFHGPKGVGILYCAPHHFD
                     SLLHGGDQEEKRRASTENIIGIAGMSQALTDATTNTLKNWTHISQLRTTFLDAISDLD
                     FYLNNGQDCLPHVLNIGFPRQNNGLLLTQLDLAGFAVSTGSACTAGTVEPSHVLTSLY
                     GANSPRLNESIRISFSELNTQEEILELAKTLRKIIGD"
     gene            11676..12023
                     /locus_tag="VBC28_001500"
     CDS             11676..12023
                     /locus_tag="VBC28_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HEO6815476.1"
                     /translation="MAFEKEINLPDCNYIYTISPNIKKYTLRDTTFTQNRLGNYELER
                     LLETVPNSGDGFPLKITINKELSGFKLLITDKSGLRIVNIFKSTDHKILQDKFYFLMD
                     SLVERNIFTKTAK"
BASE COUNT         4040 a         2367 c         1897 g         3739 t
ORIGIN      
        1 tacatttatt atattgcttt tctttttcga agataagtta atcctacaat agctaagata
       61 acaacaatga taacgaatag aactgcaaat tgactaccga ttatagctcc atttttcgga
      121 atagctactt gtccgatggt aaaaatttgt gaggctactg cggttgcaaa agctcccgca
      181 aattgttgta gtgtatttaa gatactatta ccatccgctg actgtgctgg ctttagtttt
      241 gttaaagctg tcgtaaccat attacttgct ccaatcccca taccaatttt agtaagaata
      301 tcaaaggcta aaagtactac aacaaacttc gcaggaatga atatagctgt accaattaaa
      361 cctatcaaag aaagggagat acctatcata attggcttaa atgcaccaat tttatccagg
      421 agataacctg caaaagggga taaaaaaact actgctattg cacctggaaa cataaacaaa
      481 ccagctttgg ttgaagtttg atgtaaaaca agttgcaaga ggttaggtaa taagaacgaa
      541 agtgctagag gaatcatttg aaaagccagg acgccataca ataagattga gaaatcttta
      601 tctttaaaaa cattcaaatt caaaaattct agctgtttat ttaaatagta aaaaataaca
      661 aaactaacaa tagctactaa taagtaaaaa atagacatct tttcaatagc taaaagaagg
      721 gttgccaagc caatacctaa actgacaaag gcaggaaaat tgagtttatc attaattctt
      781 acttgacgtt tgggaataga agccaaacct ataaaggtag atatgataag tattggtgcc
      841 agaagcataa aaatcatctt ccatcctaac attcctgaaa tgacaccacc ataagtaggt
      901 ccaactgccg gtgctaaaag tgtgaccata gcagctactc ccatcattag tcctctacgc
      961 tccatcggga tttgtgtaag aatgatatga aacaataaag ggagggctaa tcctgtccca
     1021 acaccttgca agactctccc tactaacaat atagctaaat taggggcaaa gctatcaatt
     1081 aaaacaccag ataaaaaaag cacatttgcc aatgtaaaaa tttgtctctc tgataaattt
     1141 ttaatcataa atgctgacaa aggcacagtc acagcaacag ctaacaaatt tcctgttgtc
     1201 acccattgaa taacagcggg gttaacccca aattctttca tcaaaagcgg aaaagtgaca
     1261 ttcatagatg tttcaataag aatccctgaa aaacctaata tagccgttgt aattatggca
     1321 ggcataatac gccatccctt tttagtcatt aaaatctcct tactttgatt attatcataa
     1381 aatagagcac cccgctaaat caaggtgctc catttaattc attagaatta aaattaaatt
     1441 gtttatatta gttgtcaaag gacaatcaac agttcttatt atatcaaata aagctaagaa
     1501 aaacaaagag ttttttgaat attaatgcct tcactttctt atctattctc tacccatcaa
     1561 tcaccacttc agagaacata acaattgcaa tttactctta ataccgttat catgttcata
     1621 tgctaatacc caagaaaata gtggcttttg cctcaaatga atctgaaaat aatttcttta
     1681 ttacgttaaa gacttacttt aattatttgt tttctattca aatcattaca gatatttcaa
     1741 ctctcaatca tgcagatttt gacggtagct ttgcattcca tgatatagaa actagcatac
     1801 ctcatctggt aattgactcc aactacttag cgattagtca gactaactca aaaacagagg
     1861 ctaacgatat taaaacgttc tcggaactct ccaaaacaat gaccgagttt cactatatgc
     1921 taaattttga cttatttaac catctcccat atcgttttag attgcataat aaggacggtc
     1981 aaacaattta ttcgaaccat aaacctgaag atccttttga tatttatcct gaagaagaat
     2041 atcctataga caaatgggtt caaaacagtc taatagagaa aaaagcgaaa gagctacatt
     2101 tactcttacc tggcgcatca caagattata ttctagtaca atcttataaa cgcttagaaa
     2161 atgatagtgg gcaactagtt ggttatattg aacatgtcca taatattaaa ccactattag
     2221 aggggtattt aaaagaatcc ggtcaagcta tcgttggatg gtctgatgtc acgtctggtg
     2281 cttctatctc taacgatgat tttgaactat aatccactcg tcactctaaa aagaaaacta
     2341 ataactaaaa ggcactggtt taattccagc gccttttatt aatcaatctc cagttgttgt
     2401 aagttcaatt ttttctttaa ttggtagacc tcgattagct gtaaaaatca aaggaactac
     2461 ttctgttgta acttcggaaa attgtagacc aaaccatcgt attggtgttg ctgtccaatg
     2521 ctttacagat gatttcatta gcataacaaa tcgttcacca ggttttaggt tttgactgct
     2581 tgaaaaacgt tcatcaagga caataaacct aaaatctcct actttactat caggtctaac
     2641 gctgtatgtc tttccttgtt taggcagacg tccactttca agtaattctt ctacaatcgt
     2701 acgaagatag cgtgacacag tctgacgcat cttaaatcca aggtaaagtt ctaccttcac
     2761 aatgaagtcg gttcccatca tgtcaacttt atagtcagct gtataaggtt catcagtgac
     2821 cttaatgttt acaaaccaat aaacttgtgc ttttttaggg cgcttatcaa ggatagaata
     2881 catgatagac ttatcaatca tgtctccctc catacgattt gtcaaataaa caacgttagt
     2941 atgataaatc ggataacgat gatcatgtct aagtttatct aattgtccga tgtaatcttt
     3001 taaatccaac aacttaacat aacgcgaaac aattttacta cctttatacc agatagtcat
     3061 gatgaatata attgctactg ctataataac aacaacatag cctccatgca taaatttaac
     3121 agctgaagca aggaagaaga ttccctctaa aataccaaag aaaatcatca tcaataagac
     3181 taaacctctt tttactcctt tttgaattaa aaagaaagac agtaaaatag ttgtcattag
     3241 catcgtaatt gttatcgcta atccatatgc tgcttccatg tgcgctgaag tcttaaaaag
     3301 caaaacaata gaggttgtaa tggcaaataa gaaccaatta ataacaggta tgtaggtttg
     3361 accaatattg tcaccaggat aagttgaacg aaattgtggg aatattttta gtcgcatagc
     3421 ttcagaaact aaggtaaatg atccagaaat cagtgcctgt gaagcgatga tagctgccaa
     3481 agtagctaaa ataacgacat gcattgtaaa ttgcgaagga atactagcaa aaaagggatt
     3541 caattcattt cctgcgttct tattagctaa aatccatgcc ccttgcccac aataagaaag
     3601 tataatggca accttaacga acggccatga aacatgtata tttccacgcc caacatgacc
     3661 taagtcagag tatagtgctt ctgcccccgt tgtcgccagg aaaatagatc ctaaaataaa
     3721 aataccttta tgattctctg gactaaataa caatttcaaa ccgtagtatg gattaattgc
     3781 tttgaaaact tctggatggg caaaactatt aaggagaccg ctaataccta aaaaggcaaa
     3841 ccatataaac ataattggcc caaataattt cccaataaca cccgttccaa acctttggat
     3901 ggcaaaaagt aacagtaaaa taaacaaggt cgcaaaaata acattactct gattctggaa
     3961 aatatgctgc aaactaggaa ctacttttaa tccttcaacg gctgaagtta cggttacagc
     4021 tggcgtcaaa gctccatctg acaacaaggt tgcacctcca ataacagccg gaacaattaa
     4081 ccaaggtgtc atttttctaa caagggtata taaagaaaaa ataccacctt cgtggtgatt
     4141 atccgccttt aaagctacaa gcacatactt gatagttgta ataagtgtca aggtccatat
     4201 gattaaagat atagaaccta agataaacga ttctgtgaca ctagaaatac caccttggtt
     4261 ttcaaccaat gattgcatcg tatagagtgg gcttgtacca atatctccat aaacaatgcc
     4321 taaagcaata ataaatcctg cttttgatgc tttatcaaaa gaagaatgat tgacatgttg
     4381 catttctgcc aaccctccga ttaaataata ttagtcaaaa aaatggctga actcattata
     4441 gcagattaag ttaaaaaaac aacacttatc tataaaaaga ccagaaatta ttaaagcgtt
     4501 tcctggtctt tactatttta ataacgataa acaggttgag gtccataagt ttgactgact
     4561 ggcattattt ctatgcgatt aatattaatg tgttctggtt ggctagcaac ccatgacacg
     4621 gtattagcaa tatcctctgc ttgaattgca tgcgcacctt cataaagttt ttcaactctt
     4681 ttatgatctc ctttgaaacg tacagttgaa aattctgtcc cttcacataa accgggttct
     4741 aggttagtaa ctctgatttt agttccagct aaatcggcac gcaagttgag agaaaattgt
     4801 tttacaaaag cttttgatgc gccatagaca ttagctcctg gataaggaat tgttcctgcg
     4861 gttgatccca aattgataat taaccctcta ttaacttcta ccattttcgg taaaatgcat
     4921 cgtgtcaagt aaattaatcc tacaacgtta gtattaatca tagtcatcca atcttcaaaa
     4981 tcagcttcat atgatttatc caatccaagt gctaaacctg cattattaac caacaaatca
     5041 attgattgca aatttttagg cagactttct aaagctttgt caaccgattc tctacttgta
     5101 atatccattt gaagtggata aaagtaaccc tctgaaaact tttctccaag gcattttaat
     5161 ttatccattc gacgagcaca accaataacg ctataaccat ctgaaactaa tttagtaaca
     5221 atagctgcac caaaaccagc agatgccccc gtcacaagcg caactttacg tgatatcata
     5281 aatactcctc ctctagtata tagaaaaacg agctaggaaa aatccttctc gttttataac
     5341 ttattttgat ttctcatcaa tagcttgagc agctgtaatg atagcaagtt tgtaaatatc
     5401 ttcagcactt gatccacgtg ataaatcatt tactggagca ttcaatcctt gtaagatcgg
     5461 tccgattgct tcaaacatac ccaaacgttg tgcaatttta tagccaatat ttcctgattg
     5521 aaggtctggg aaaataaaaa catttgcttt tccagcaaca tcactattag gagctttaat
     5581 ttctgcagtt tccggaacaa aagctgcatc aaattgtaat tctccatcaa cagctaattc
     5641 gggagataaa tctttggcaa ttttagctgc ttcttgaact ttttcagctt gaggcgcttt
     5701 tgcacttcct tttgttgaaa aactaagcat agcaatcttg ggattaatat caaatatttt
     5761 ggctgtatcc gcagtattta ctgcaatttc tgctagctct tgtgcattag gatcaatgtt
     5821 aatagcacag tcagcaaaaa tataacgttc ttgtgtactt tcacggttca tcaagaatac
     5881 acctgaagtg cgtgaaattc ctggttttgt tttaataatt tgaagagcag gacgtacagt
     5941 atcagctgtt gaatgaatgg cccctgatac cataccatct gccaaaccaa gttttactaa
     6001 cataacccca aaatagttaa catccttcaa aagacgatct gcatcttcca tagtagcttt
     6061 gcctttacga atctcaacaa aagcttcttt catctcttca aatttttcgt attgttcagg
     6121 gtcaataacg ctataatctt gatcaacaaa accaagtctt gccaaagaat cgtgaacttc
     6181 gtcagcctta cctaaaataa caggatccac aagaccttca aatttcaaac gagctgctgc
     6241 acgtaataca cgttcatcgc ttccctcagg gaaaacaatc ttaacataat cacttgcaac
     6301 tttttctctt aaaccaccaa ataaggaacg catatatata aaaccttcta tttctaaatt
     6361 aatgtaattc catgataacg ctgtcaaagt catctgtcaa gtctagcgtc tgtttattta
     6421 tttttcccat aaaagggtcg taaaaagaca aagaatgaca atgcaatgct tgcctgttta
     6481 ttcctagatc catacgtcct ccgtacaaat catctcccaa aagaggaaag ccgatatgag
     6541 caaagtgaac tctaatttga tgagtacgcc ctgtatgtaa tttgatatcc actaatctaa
     6601 catcaccata acgagcaact acttgataac tagtatgtgc atatttgcct gattcatgga
     6661 ctcgcctcgt aataatacta tcaacatctc gtgcaatagg agctataata tcaccactat
     6721 ctgctaaatc accactccct gatactaatg cataatacct tttttcaatt gctttagcct
     6781 gaagttgttt atctagacga gcatgggcat aaccatgctt ggcgaataac attaatccac
     6841 ttgtatctcg atccaaccgg gtcacaatat ggacctgctg attggcatag ttattagaca
     6901 cataatagtg ttttataaaa ttagcaattg tatttgaatg aatagagcta ggaatactag
     6961 cgaaaccaaa aggtttattt aaaatcaaaa aatgatcatc ctcataaaca atatctaaat
     7021 cgtgaggtac tggttccaaa gtttcgtgag aaggctcgtc aggaatatct atcgtaacaa
     7081 catcgccaat ctctaataag aaaatagcgt tctgctcttc accattgaca aaaatcttac
     7141 cacctctgta cttaatcttt gctaataacc cccttgaaac atcatgactt ttcaaaagtg
     7201 ttttaacttt gcacctttct tctgctatgt attcaaattt caatgttcag gttctccgat
     7261 aaaagcatcg ttaacacgtt cccaaaagct agtgtgactt ggagttgaaa caaagttaat
     7321 acttttttcg tcaatactat attctatttt ggtaacattt ttgtaatgga ccgttttgtt
     7381 atctatagaa attgtataga caccaactcg tttaggaact atctctatag catctttttt
     7441 ggggatgata accgatgacc ccaaggtacg atagacgcga ttattaagac tagaaatttc
     7501 tgttagttgc aaggcttcta tcgttgggtg gagtactgcc ccccctagtg atttattgta
     7561 agcagtacta ccggttggtg tagaaactaa aatgccatct cccctaaacc tttcaaatac
     7621 cacttgatta ataacaacat ccgctaccat tgttttttca atacgcttaa tagtagactc
     7681 atttaaagca cgcgcacgaa taactcgacc atcttcaagg gtaatagtta ctttcaaaat
     7741 gggataggag atttgttcac cttcatcatt tttcaaatta ttaattaatg tgtcaacttc
     7801 aaaatctcga tagtctgtat aaaaccctaa atgtcctgta tgaacaccga caaaccttac
     7861 tttatccagt tgtttttcgt acatatggaa ggctgacaaa agcataccat ctccaccaat
     7921 agaaatgaca atatcaggat ccttttttga taaataaaaa tcaggatcat gtttaaaagc
     7981 agcaaagagc tttgaggcta cacgcttgct ttgatatttt ccgttagcaa ttatagcaac
     8041 acgtgttgct ctatctgtaa aattcatctg tgtcatcact atttcctaca ccatcgctca
     8101 attttcgatt taaaggatca aataaaagct gtgcttcacg aatatcttcg cgaatcttac
     8161 gcatttcttc atccaattct aaagcgattt ttgctgtttt ctccagtctt tgtttaattt
     8221 cttcagggaa gtccccctga tacttgtaat ttaatgaatg ttcgatggtt gcccaaaaat
     8281 tcatagccag agttctaatt tgtatttctg ctaaaacttt cttttgtcca tctattgtgt
     8341 ctacaggata ctctacaacg acatgatatg aacgatatcc actagatttc atatgggtaa
     8401 tatagtctcg ctcctgaacc actgtcatgt catgacgttt tctcaataag gcaaggactt
     8461 catcaacgtc atctacaaat tgtaccatga tacgaagacc tgcaatatct tgcaagtcct
     8521 gagcaagatt ttcttctgaa ataccacgta aaaccatctt ttcttgaata ctttcaaccg
     8581 actttacgcg tccagtaaca aattctattg gagagtgacg attttgcttt ctaaattgtt
     8641 tacgaatacc acgaagcttt atttttaatt caccaaccgt ttgaatataa gggtctagaa
     8701 atgtttccca atccattgac attctaatcc tccaaaatat ttaatgtact ctcttgtaaa
     8761 ataaactttt tttctataaa attaacacaa caattaatat tataccatat ttaggaaaag
     8821 gaagtgataa gatgacacac ctagaaattg aatacaaaac tctattgaat aaagacgaat
     8881 ttaatcgtct aacaagctta ttttctcatg ttcaacctat tactcaaacc aattactatt
     8941 ttgacacaga aacatttgaa atgaaagcac accgaatgtc acttcgcatt agaacgttac
     9001 ctaaccgtgc cgaactaaca ctaaaaatcc ctagagaagt aggaaattta gagcacaatc
     9061 atgaccttac tttagaagaa gctaaatata tcgttaaaaa tggccagttc cctgaagata
     9121 ctgagattgc ttctttaatc cttgaaaagg gagttgatcc aaccaaatta gctgtctttg
     9181 gacaacttac aacaacaaga cgagagatgg aaacttcaat cggcttaatg gctctagaca
     9241 gcaatatata tgctgatatt aaagattatg aacttgagtt agaagtcaaa caacctaaac
     9301 aagggaaaag agatttcgac cagtttttaa aagaaaataa tattaatttt aaatacgcca
     9361 aaagtaaggt ggctagattc tctgctactt taaaaaataa ctaacgttga gagattggat
     9421 tttccaatct cttttataat tctcatttaa gcaagaactt aaaatctcat actatctctc
     9481 ctcaacattg ttattgcaag cattttcatg tttcttatcg tgaatttttt ctattttttt
     9541 ggtaaactat tagtatcaat ctaaaggagt catctatctc atggcagaac aatacgccga
     9601 taaacaaatc aaacttttct cattaaccgc taatagagaa attgctgaaa aaatttccca
     9661 agcttcagga attcctttgg gtaagatgtc atcacgtcaa ttctctgatg gcgaaattat
     9721 gattaatatt gaggaaacgg tgcgcggtga cgatatctat atcatccaat ctacaagctt
     9781 cccagtcaat gataatctct gggaattact cattatgatt gacgcttgca aacgtgcaag
     9841 tgctaatact gttaacatcg tagtccctta ttttggatac tctcgtcagg accgcatcgc
     9901 tgcatctcgt gaacctatta ctgcaaaatt ggtcgctaat atgttagtta aagctggagt
     9961 tgatcgagtt cttaccctcg acttacatgc agtgcaagta caagggttct tcgatatccc
    10021 agttgacaat ctttttactg tcccactatt tgctgaacac tataatcaac tcggtctctc
    10081 tggtgaagat gtcgtcgttg ttagtcctaa aaattctgga atcaagcgtg ctcgtagttt
    10141 ggcagaatac cttgactctc caattgctat cattgattat gcacaagatg attctgaacg
    10201 tgaagaaggc tatattattg gtgaggtaga aggtaagaaa gctattatta ttgatgatat
    10261 tttaaataca ggaaaaacct ttgccgaagc tgcaaaaatt ttagaacgtg gtggagcaac
    10321 tgagatttat gctgttgcta gtcatgggct ttttgctggc ggcgcagctg atattttaga
    10381 gtctgcacca attagagaaa ttatcgtaac agactctgtt ttatctaaag agcgtattcc
    10441 tagcaatatt aaatatctga ccgctagcca tctcattgca gacgctatta tccgtatcca
    10501 tgaaagaaaa cctttaagtc cattattttc atatcgttca gataagaagg attaattgat
    10561 gatttattta gacaatgctg ctactaccgc tctaacccca tctgttattg agaaaatgac
    10621 caatgtcatg acaagtaact atggtaatcc atctagtata catacctttg ggcgtcaagc
    10681 aaatcaactt ttacgtgaat gtcgacaaat tattgctgaa tatctaaatg ttaattcacg
    10741 tgaaattatt ttcacttctg ggggaactga gagcaacaat acagctatca aagggtatgc
    10801 tcttgcaaat cagctaaaag gtaaacatat tattacctct gaaattgaac atcattcagt
    10861 cctacatact atgacttact tatcagagcg atttggtttt gatattactt acttaaaacc
    10921 aaaccatgga caaattactg caaaagacgt tcaagaagct ttacgagatg atactattat
    10981 ggtatctctc atgtttgcta ataatgaaac cggggacttt ttaccaattc aagagattgg
    11041 tcagcttctc aggaaccacc aagctgtttt tcacgttgat gccgttcaag tctttagcaa
    11101 aatggaactt gatcctcatt ctttaggaat tgacttttta gctgcttctg cccataaatt
    11161 tcacggtcca aaaggtgttg ggatacttta ctgtgctccc catcactttg atagtctact
    11221 tcatggtgga gaccaagagg aaaaaaggcg tgcttcaact gaaaatataa ttggtattgc
    11281 tggaatgtct caagctctta ctgatgctac gactaacacc cttaaaaatt ggactcacat
    11341 tagtcagctg agaacgacct ttttagatgc tatttcagac cttgacttct atcttaataa
    11401 cggtcaagac tgcttacctc atgtacttaa tataggtttt cctagacaga ataatggctt
    11461 gttattgaca cagttagatt tagctggatt cgcagtttca acaggttctg catgtactgc
    11521 aggaacagtc gaacctagtc atgtcttaac aagcttgtat ggagccaact caccacgtct
    11581 aaatgaatca atacgtatta gtttttcaga actaaatacc caagaagaaa ttcttgaatt
    11641 agctaaaacc ttaagaaaaa ttataggaga ttaatatggc atttgaaaaa gaaataaatc
    11701 ttccagactg caattacatt tacactatca gcccgaacat taaaaaatat accttacgcg
    11761 atactacatt cactcaaaat cgcttaggta attatgaatt agagcgtctc ctagaaacag
    11821 tacctaattc tggcgatggc tttcctctaa agattacaat caataaagaa ctttctggtt
    11881 ttaagctctt aattactgat aaatctggcc ttagaatagt aaatatcttt aaatctaccg
    11941 atcataaaat tcttcaagac aaattttatt tcttaatgga cagcctcgtt gagcgtaata
    12001 tctttacgaa aactgcaaaa taatttaccg accactattt tta
//
LOCUS       DATRMA010000058        11993 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.058, whole genome shotgun sequence.
ACCESSION   DATRMA010000058 DATRMA010000000
VERSION     DATRMA010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11993
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.058"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            1..604
                     /locus_tag="VBC28_001501"
                     /pseudo
     CDS             1..604
                     /locus_tag="VBC28_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224533.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="UPF0236 family protein"
     gene            1259..4711
                     /gene="scpA/B"
                     /locus_tag="VBC28_001502"
     CDS             1259..4711
                     /gene="scpA/B"
                     /locus_tag="VBC28_001502"
                     /EC_number="3.4.21.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021340616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C5a peptidase ScpA/B"
                     /protein_id="HEO6815477.1"
                     /translation="MRKKQKLPFDKLAIALISTSILLNAQSDIKANTVTEDTPATEQA
                     VEPPQPIAVSEESRSSKETKTSQTPSDVGETVADDANDLAPQAPAKTADTPATSKATI
                     RDLNDPSHVKTLQEKAGKGAGTVVAVIDAGFDKNHEAWRLTDKTKARYQSKENLEKAK
                     KEHGITYGEWVNDKVAYYHDYSKDGKNAVDQEHGTHVSGILSGNAPSEMKEPYRLEGA
                     MPEAQLLLMRVEIVNGLADYARNYAQAIRDAVNLGAKVINMSFGNAALAYANLPDETK
                     KAFDYAKSKGVSIVTSAGNDSSFGGKPRLPLADHPDYGVVGTPAAADSTLTVASYSPD
                     KQLTETATVKTDDHQDKEMPVISTNRFEPNKAYDYAYANRGTKEDDFKDVEGKIALIE
                     RGDIDFKDKIANAKKAGAVGVLIYDNQDKGFPIELPNVDQMPAAFISRRDGLLLKDNP
                     PKTITFNATPKVLPTASGTKLSRFSSWGLTADGNIKPDIAAPGQDILSSVANNKYAKL
                     SGTSMSAPLVAGIMGLLQKQYETQYPDMTPSERLDLAKKVLMSSATALYDEDEKAYFS
                     PRQQGAGAVDAKKASAATMYVTDKDNTSSKVHLNNVSDKFEVTVTVHNKSDKPQELYY
                     QVTVQTDKVDGKHFALAPKALYETSWQKITIPANSSKQVTVPIDASRFSKDLLAQMKN
                     GYFLEGFVRFKQDPTKEELMSIPYIGFRGDFGNLSALEKPIYDSKDGSSYYHEANSDA
                     KDQLDGDGLQFYALKNNFTALTTESNPWTIIKAVKEGVENIEDIESSEITETIFAGTF
                     AKQDDDSHYYIHRHANGKPYAAISPNGDGNRDYVQFQGTFLRNAKNLVAEVLDKEGNV
                     VWTSEVTEQVVKNYNNDLASTLGSTRFEKTRWDGKDKDGKVVANGTYTYRVRYTPISS
                     GAKEQHTDFDVIVDNTTPEVATSATFSTEDSRLTLASKPKTSQPVYRERIAYTYMDED
                     LPTTEYISPNEDGTFTLPEEAETMEGATVPLKMSDFTYVVEDMTGNITYTPVTKLLEG
                     HSNKPEQDGSDQAPDKKPEAKPEQDGSGQTPDKKKETKPEKDSSGQTPGKTPQKGQSS
                     RTLEKRSSKRALATKASTRDQLPTTNDKDTNRLHLLKLVMTTFFLGLAAHIFKTKRQK
                     ETKK"
     gene            5293..6570
                     /gene="ltrA"
                     /locus_tag="VBC28_001503"
     CDS             5293..6570
                     /gene="ltrA"
                     /locus_tag="VBC28_001503"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001901474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HEO6815478.1"
                     /translation="MSELLDKILSRNNMLEAYKQVKSNKGSAGIDGVTIEQMDDYLHQ
                     NWRETKQLIKERSYKPQPVLRVEIPKPNGGVRNLGIPTAMDRMIQQAIVQVLSPLCEK
                     HFSEYSYGFRPNRSCETAIVQLLEYLNDGYEWIVDIDLEKFFDTVPQDRLMSLVHNII
                     QDGDTESLIRKYFHSGVVINGQRHKTLVGTPQGGNLSPLLSNIMLNELDKELEKRGLR
                     FVRYADDCVITVGSEAAAKRVMHSVSSYIEKRLGLKVNMTKTKIVRPNKLKYLGFGFW
                     KSPKGWKCRPHQDSVQSFKRKLKQLTMRKWSIDLITRIERLNWVIRGWINYFSLGNMK
                     SIMTQIDERLRTRIRVIIWKQWKKKAKRLWGLLKLGVARWIADKVSGWGDHYQLVAQK
                     SVLKRAISKPALAKRGLVSCLDYYLERHALKVS"
     gene            6804..7724
                     /gene="lmb"
                     /locus_tag="VBC28_001504"
     CDS             6804..7724
                     /gene="lmb"
                     /locus_tag="VBC28_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein/laminin-binding adhesin Lmb"
                     /protein_id="HEO6815479.1"
                     /translation="MKKVFFLMAMVVSLVMIAGCDKSANPKQPTQGMSVVTSFYPMYA
                     MTKEVSGDLNDVRMIQSGAGIHSFEPSVNDVAAIYDADLFVYHSHTLEAWARDLDPNL
                     KKSKVNVFEASKPLTLDRVKGLEDMEVTQGIDPATLYDPHTWTDPVLAGEEAVNIAKE
                     LGHLDPKHKDSYTKKAKAFKEEAEQLTEEYTQKFKKVRSKTFVTQHTAFSYLAKRFGL
                     KQLGISGISPEQEPSPRQLKEIQDFVKEYNVKTIFAEDNVNPKIAHAIAKSTGAKVKT
                     LSPLEAAPSGNKTYLENLRANLEVLYQQLK"
     gene            7737..10205
                     /locus_tag="VBC28_001505"
     CDS             7737..10205
                     /locus_tag="VBC28_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HEO6815480.1"
                     /translation="MKKTYGYIGSVAAILLATHIGSYQLGKHHMGLATKDNQIAYIDD
                     SKGKVKAPKTNKTMDQISAEEGISAEQIVVKITDQGYVTSHGDHYHFYNGKVPYDAII
                     SEELLMTDPNYHFKQSDVINEILDGYVIKVNGNYYVYLKPGSKRKNIRTKQQIAEQVA
                     KGTKEAKEKGLAQVAHLSKEEVAAVNEAKRQGRYTTDDGYIFSPTDIIDDLGDAYLVP
                     HGNHYHYIPKKDLSPSELAAAQAYWSQKQGRGARPSDYRPTPAPGRRKAPIPDVTPNP
                     GQGHQPDNGGYHPAPPRPNDASQNKHQRDEFKGKTFKELLDQLHRLDLKYRHVEEDGL
                     IFEPTQVIKSNAFGYVVPHGDHYHIIPRSQLSPLEMELADRYLAGQTDDNDSGSDHSK
                     PSDKEVTHTFLGHRIKAYGKGLDGKPYDTSDAYVFSKESIHSVDKSGVTAKHGDHFHY
                     IGFGELEQYELDEVANWVKAKGQADELVAALDQEQGKEKPLFDTKKVSRKVTKDGKVG
                     YIMPKDGKDYFYARYQLDLTQIAFAEQELMLKDKKHYRYDIVDTGIEPRFAVDVSSLP
                     MHAGNATYDTGSSFVIPHIDHIHVVPYSWLTRNQIATIKYVMQHPEVRPDVWSKPGHE
                     ESGSVIPNVTPLDKRAGMPNWQIIHSAEEVQKALAEGRFAAPDGYIFDPRDVLAKETF
                     VWKDGSFSIPRADGSSLRTINKSDLSQAEWQQAQELLAKKNAGDATDTDKPEEKQQAD
                     KSNENQQPSEASKEEKESDDFIDSLPDYGLDRATLEDHINQLAQKANIDPKYLIFQPE
                     GVQFYNKNGELVTYDIKTLQQINP"
     gene            complement(10404..10643)
                     /locus_tag="VBC28_001506"
                     /pseudo
     CDS             complement(10404..10643)
                     /locus_tag="VBC28_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612023.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(10683..11815)
                     /locus_tag="VBC28_001507"
                     /pseudo
     CDS             complement(10683..11815)
                     /locus_tag="VBC28_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558285.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         4013 a         2261 c         2459 g         3260 t
ORIGIN      
        1 gctatactag tagagttttt aaggaattag gaaaagcact taaggtaaag aaacatgagc
       61 atttttggga tatctatcat gttaaagaaa agttaagttc ataccttaga aaatatccaa
      121 ttgaattaac cgattttgct ttagatgcgg taaaaaaata taattctgat aagcttgaat
      181 tagtttttga tactgttgaa tcactgattt gtgatgaact tgaagatcaa gaatttcaga
      241 agtttaagaa aaaagtatta aataatttca aatatataaa accagctcat cttagaaatc
      301 tttcaaatcg tggtattggt atcatggaat cacaacacag aaagataacg tatagaatga
      361 agcgacgtgg catgtattgg tcaaagtggg gaatctccac aatggcaaat atgattatac
      421 ttgaaagagc taacggttta cgagaattat ttttcggttc ttggagaaag gtatacagtg
      481 agtataaaga aggttcatta gtgcagggcg actttttaaa aagacagatg aattagataa
      541 attttctaag ccccttctaa aaaatggcag aaaatggagt ataacaggaa tcaaaacaaa
      601 atagaaaaag tctaaagata cctttatttc tagcgttttt ttgcatgttt taacttgcat
      661 taaatcttct aaagtagtat aattatatta aagaaaagca aaaaaggtga aggagcgcga
      721 actcctacac caaaccgatt aagacaaata ccacttgctt aatcagtcga accttattgt
      781 agcataacta aaacaatacc gtcaagactg aacaagcaaa aaatacttgt tttggtcttt
      841 ttaagaataa ttcaagtttt cttaaattta tgaggtatcg actaatctca aaatttcaga
      901 cacatacctg agtgaacatc aagagagaac cgatcggttc tctcttttat gtctaaaaga
      961 atgaggttaa ggaggtcaca aactaaacaa ctcttaaaaa gatgaccttt actaataatc
     1021 gtctttgttt tataataaag atgttagtaa tataattgat aaatgagata catttaatca
     1081 ttatggcaaa agcaagaaaa atagctgtgt catatgcaaa taacccctgt ttgctcttta
     1141 aaaaagacgt tatccttatt tctctacaca cagacggata gctaggagag aatcgtttga
     1201 ttctctcttt tcttaatggt cataaagaca aagtctctca tcatgaaagg acgacacatt
     1261 gcgtaaaaaa caaaaactac catttgataa acttgccatt gcgcttatat ctacgagcat
     1321 cttgctcaat gcacaatcag acattaaagc aaatactgtg acagaagaca ctcctgctac
     1381 cgaacaagcc gtagaacccc cacaaccaat agcagtttct gaggaatcac gatcatcaaa
     1441 ggaaactaaa acctcacaaa ctcctagtga tgtaggagaa acagtagcag atgacgctaa
     1501 tgatctagcc cctcaagctc ctgctaaaac tgctgataca ccagcaacct caaaagcgac
     1561 tattagggat ttgaacgacc cttctcatgt caaaaccctg caggaaaaag caggcaaggg
     1621 agctgggacc gttgttgcag tgattgatgc tggttttgat aaaaatcatg aagcgtggcg
     1681 cttaacagac aaaactaaag cacgttacca atcaaaagaa aatcttgaaa aagctaaaaa
     1741 agagcacggt attacctatg gcgagtgggt caatgataag gttgcttatt accacgacta
     1801 tagtaaagat ggtaaaaacg ctgttgatca agaacacggc acacacgtgt cagggatctt
     1861 gtcaggaaat gctccatctg aaatgaaaga accttaccgc ctagaaggtg cgatgcctga
     1921 ggctcaattg cttttgatgc gtgtcgaaat tgtaaatgga ctagcagact atgctcgtaa
     1981 ctacgctcaa gctatcagag atgctgtcaa cttgggagct aaggtgatta atatgagctt
     2041 tggtaatgct gcactagctt acgccaacct tccagacgaa accaaaaaag cctttgacta
     2101 tgccaaatca aaaggtgtta gcattgtgac ctcagctggt aatgatagta gctttggggg
     2161 caagccccgt ctacctctag cagatcatcc tgattatggg gtggttggga cacctgcagc
     2221 ggcagattca acattgacag ttgcttctta cagcccagat aaacagctca ctgaaactgc
     2281 tacggtcaaa acagacgatc atcaagataa agaaatgcct gttatttcaa caaaccgttt
     2341 tgagccaaac aaggcttacg actatgctta tgctaatcgt ggtacgaaag aggatgattt
     2401 taaggatgtc gaaggtaaga ttgcccttat tgaacgtggc gatattgatt tcaaagataa
     2461 gattgcaaac gctaaaaaag ctggtgctgt aggggtcttg atctatgaca atcaagacaa
     2521 gggcttcccg attgaattgc caaatgttga ccagatgcct gcggccttta tcagtcgaag
     2581 agatggtctc ttattaaaag acaatccccc aaaaaccatt accttcaatg cgacacctaa
     2641 ggtattgcca acagcaagtg gcaccaaact aagccgcttc tcaagctggg gtctgacagc
     2701 tgacggcaat attaagccgg atattgcagc acccggccaa gatattttgt catcagtggc
     2761 taacaacaag tatgccaaac tttctggaac tagtatgtct gcaccattgg tagcgggtat
     2821 catgggactg ttgcaaaagc aatatgagac acagtatcct gatatgacac catcagagcg
     2881 tcttgattta gctaagaaag tattgatgag ctcagcaact gccctatatg atgaagatga
     2941 aaaagcttat ttttctcctc gccaacaggg agcaggagca gtcgatgcta aaaaagcttc
     3001 agcagcaacg atgtatgtaa cagataagga caatacctca agcaaggttc acctgaacaa
     3061 tgtttctgat aaatttgaag taacagtaac agttcacaac aaatctgata aacctcaaga
     3121 gttgtattac caagtaactg ttcaaacaga taaagtagat ggaaaacact ttgccttggc
     3181 tcctaaagca ttgtatgaga catcatggca aaaaatcaca attccagcca atagcagcaa
     3241 acaagtcacc gttccaatcg atgctagtcg atttagcaag gacttgcttg cccaaatgaa
     3301 aaatggctat ttcttagaag gttttgttcg tttcaaacaa gatcctacaa aagaagagct
     3361 tatgagcatt ccatatattg gtttccgagg tgattttggc aatctgtcag ctttagaaaa
     3421 accaatctat gatagcaaag acggtagcag ctactatcat gaagcaaata gtgatgccaa
     3481 agaccaatta gatggtgatg gattacagtt ttacgctctg aaaaataact ttacagcact
     3541 taccacagag tctaacccat ggacgattat taaagctgtc aaagaagggg ttgaaaacat
     3601 agaggatatc gaatcttcag agatcacaga aaccattttt gcaggtactt ttgcaaaaca
     3661 agacgatgat agccactact atatccaccg tcacgctaat ggcaaaccat atgctgcgat
     3721 ctctccaaat ggggacggta acagagatta tgtccaattc caaggtactt tcttgcgtaa
     3781 tgctaaaaac cttgtggctg aagtcttgga caaagaagga aatgttgttt ggacaagtga
     3841 ggtaaccgag caagttgtta aaaactacaa caatgacttg gcaagcacac ttggttcaac
     3901 ccgttttgaa aaaacgcgtt gggacggtaa agataaagac ggcaaagttg ttgctaacgg
     3961 aacctacacc tatcgtgttc gctacacgcc gattagctca ggtgcaaaag aacaacacac
     4021 tgattttgat gtgattgtag acaatacgac acctgaagtc gcaacatcgg caacattctc
     4081 aacagaagat agtcgtttga cacttgcatc taaaccaaaa accagccaac cggtttaccg
     4141 tgagcgtatt gcttacactt atatggatga ggatctgcca acaacagagt atatttctcc
     4201 aaatgaagat ggtaccttta ctcttcctga agaggctgaa acaatggaag gcgctactgt
     4261 tccattgaaa atgtcagact ttacttatgt tgttgaagat atgactggta acatcactta
     4321 tacaccagtg actaagctat tggagggcca ctctaataag ccagaacaag acggttcaga
     4381 tcaagcacca gacaaaaaac cagaagctaa accagaacaa gacggttcag gtcaaacacc
     4441 agataaaaaa aaagaaacta aaccagaaaa agatagttca ggtcaaacac caggtaaaac
     4501 tcctcaaaaa ggtcaatctt ctcgtactct agagaaacga tcttctaagc gtgctttagc
     4561 tacaaaagca tcaacaagag atcagttacc aacgactaat gacaaggata caaatcgttt
     4621 acatctcctt aagttagtta tgaccacttt cttcttggga ttagcagctc atatatttaa
     4681 aacaaaacgc caaaaagaaa ctaaaaaata gtattactta aactgataag agcttattta
     4741 atagagagaa ccaatttggt tctctctatt tttttatagt acgacgggca tgtcgtacat
     4801 ctgaggtgca agtcctctgt gggcacccgc taccggtgaa cccaatagcg actcccaagc
     4861 ttgactatcg tgaggtagcg gggagaggaa gggatagcga aatcgtggct ctacgaacag
     4921 aaacgtgata tgaaggcgta tatagcggat gagggggcta taaactctaa agtccaaata
     4981 ggtagtcgta acctatatac gtaaatcacg agagtaaacc aggaaagatg tccgacttat
     5041 cccgtgaggt cctgtcgagg gtaaaaccta gtaacaacga acgacaagaa gtcagccgaa
     5101 cccatagtag tgatgaagtt tctgtaatgg aaatggaacg aaggggtgaa caactaatcg
     5161 aagtaatcct acttcacttg tgtctgtaaa acaaacggtc tgatagaact agatgcggtt
     5221 acgtattgac tagaggaagg ttcaccaatg taagatatca tgcagacaac gaaaaaagaa
     5281 aggaatacgc acatgtcaga attgctagat aagatattat ctcggaacaa tatgcttgaa
     5341 gcttacaagc aagtgaaatc aaacaaaggt tctgctggta tcgatggggt cactatcgag
     5401 cagatggatg actatcttca ccaaaattgg cgagaaacca agcaactcat caaagagagg
     5461 agctataaac ctcaaccggt tctcagggtt gaaatcccaa aaccaaacgg aggagttcgt
     5521 aacctaggta tcccgacggc tatggataga atgattcagc aggccatcgt tcaagttttg
     5581 agtccactct gcgaaaaaca tttttcagag tatagctatg ggttcagacc caatcgctcc
     5641 tgcgaaacag ccattgttca gctacttgag tatttaaacg atggctacga gtggattgtg
     5701 gacattgact tggaaaagtt cttcgatact gttccgcaag acagattgat gtccctggtt
     5761 cataatatca ttcaagatgg cgatacggag tcactgattc gtaagtactt ccattcggga
     5821 gttgttatta acggacagcg acataagact ttagtcggga cacctcaagg cgggaatcta
     5881 tcacccctcc tatctaatat tatgcttaat gagttagaca aagagttgga aaagcgaggt
     5941 cttcgctttg tccgttacgc cgatgactgt gtcatcactg tcggaagcga agcagctgct
     6001 aagcgggtca tgcattcggt cagtagctat attgagaagc gattagggtt gaaagtcaac
     6061 atgactaaga ccaagattgt cagaccgaac aaactcaaat acctcggatt tggtttttgg
     6121 aaatctccaa aaggttggaa gtgtcgtcct caccaagaca gcgttcagag ctttaagcga
     6181 aaactgaagc aactgacgat gaggaaatgg agcattgacc tgataactcg cattgaacga
     6241 ttgaactggg tcattcgagg atggataaac tatttctcgc ttggcaatat gaagagtatc
     6301 atgacacaaa tagatgagcg tctgcgaacc cgtattcgag tgattatctg gaagcaatgg
     6361 aagaagaaag caaagcgcct atggggactc ttaaaactag gagttgctag atggatagcc
     6421 gataaagttt ctggatgggg tgaccactat cagttggtag ctcagaagtc ggtactcaaa
     6481 cgtgctatat caaaaccagc cctcgcaaag cgaggactgg tcagttgctt agattactat
     6541 cttgaacgac atgcgttaaa agttagttga accgccgtat gccgaacggc acgtacggtg
     6601 gtgtgagagg ggctagagat tagcctctac tcgattgtgc tacctcatca aaaagaagtt
     6661 aggacaaata gctgtcaaca attgcctttt gcttatccct tcagttgatg tgataaaaga
     6721 tggtagttgt cattgacaaa gcattgataa aggagtaaaa ttaactggtt aataactggt
     6781 taaattataa ttgaggaggt actatgaaaa aagttttttt tctcatggct atggttgtga
     6841 gtttagtaat gatagcaggg tgtgataagt cagcaaaccc caaacagcct acgcaaggca
     6901 tgtcagttgt aaccagcttt tacccaatgt atgcgatgac aaaagaagta tctggagacc
     6961 taaatgatgt gaggatgatc caatcaggtg caggcattca ttcctttgaa ccgtctgtaa
     7021 atgatgtggc agctatttat gacgcggatt tgtttgttta ccattcacat accttagaag
     7081 cttgggcaag ggatctagac cctaatttaa aaaaatcaaa ggttaatgtg tttgaagcgt
     7141 caaaacctct gacactagat agagtcaaag ggctagaaga tatggaagtc acacaaggca
     7201 ttgaccctgc gacactttat gacccacata cctggacgga tcccgtttta gctggtgagg
     7261 aagctgttaa tatcgctaaa gagctaggac atttggatcc taaacacaaa gacagttaca
     7321 ctaaaaaggc taaggctttc aaagaagaag cagagcaact aactgaagaa tacactcaaa
     7381 aatttaaaaa ggtgcgctca aaaacatttg tgacgcaaca cacggcattt tcttatctgg
     7441 ctaaacgatt cggcttgaaa caacttggta tctcgggtat ttctccagag caagagccct
     7501 ctcctcgcca attgaaagaa attcaagact ttgttaaaga atacaacgtc aagactattt
     7561 ttgcagaaga caacgtcaac cccaaaattg ctcatgctat tgcgaaatca acaggagcta
     7621 aagtaaagac attaagtcca cttgaagctg ctccaagcgg aaacaagaca tatctagaaa
     7681 atcttagagc aaatttggaa gtgctctatc aacagttgaa gtaaaggaga ttattagtga
     7741 agaaaacata tggttatatc ggctcagttg ctgctatttt actagctact catattggaa
     7801 gttaccagct tggtaagcat catatgggtc tagcaacaaa ggacaatcag attgcctata
     7861 ttgatgatag caaaggtaag gtaaaagccc ctaaaacaaa caaaacgatg gatcaaatca
     7921 gtgctgaaga aggcatctct gctgaacaga tcgtagtcaa aattactgac caaggttatg
     7981 ttacctcaca cggtgaccat tatcattttt acaatgggaa agttccttat gatgcgatta
     8041 ttagtgaaga gttgttgatg acggatccta attaccattt taaacaatca gacgttatca
     8101 atgaaatctt agacggttac gttattaaag tcaatggcaa ctattatgtt tacctcaagc
     8161 caggtagtaa gcgcaaaaac attcgaacca aacaacaaat tgctgagcaa gtagccaaag
     8221 gaactaaaga agctaaagaa aaaggtttag ctcaagtggc ccatctcagt aaagaagaag
     8281 ttgcggcagt caatgaagca aaaagacaag gacgctatac tacagacgat ggctatattt
     8341 ttagtccgac agatatcatt gatgatttag gagatgctta tttagtacct catggtaatc
     8401 actatcatta tattcctaaa aaagatttgt ctccaagtga gctagctgct gcacaagcct
     8461 actggagtca aaaacaaggt cgaggtgcta gaccgtctga ttaccgcccg acaccagccc
     8521 caggtcgtag gaaagcccca attcctgatg tgacgcctaa ccctggacaa ggtcatcagc
     8581 cagataacgg tggttatcat ccagcgcctc ctaggccaaa tgatgcgtca caaaacaaac
     8641 accaaagaga tgagtttaaa ggaaaaacct ttaaggaact tttagatcaa ctacaccgtc
     8701 ttgatttgaa ataccgtcat gtggaagaag atgggttgat ttttgaaccg actcaagtga
     8761 tcaaatcaaa cgcttttggg tatgtggtgc ctcatggaga tcattatcat attatcccaa
     8821 gaagtcagtt atcacctctt gaaatggaat tagcagatcg atacttagcc ggccaaactg
     8881 atgacaacga ctcaggttca gatcactcaa aaccatcaga taaagaagtg acacatacct
     8941 ttcttggtca tcgcatcaaa gcttacggaa aaggcttaga tggtaaacca tatgatacga
     9001 gtgatgctta tgtttttagt aaagaatcca ttcattcagt ggataaatca ggagttacag
     9061 ctaaacacgg agatcatttc cactatatag gatttggaga acttgaacaa tatgagttgg
     9121 atgaggtcgc taactgggtg aaagcaaaag gtcaagctga tgagcttgtt gctgctttgg
     9181 atcaggaaca aggcaaagaa aaaccactct ttgacactaa aaaagtgagt cgcaaagtaa
     9241 caaaagatgg taaagtgggc tatattatgc caaaagatgg caaggactat ttctatgctc
     9301 gttatcaact tgatttgact cagattgcct ttgccgaaca agaactaatg cttaaagata
     9361 agaagcatta ccgttatgac attgttgata caggcattga gccacgattt gctgtagatg
     9421 tgtcaagtct gccgatgcat gctggtaatg ctacttacga tactggaagt tcgtttgtta
     9481 tcccacatat tgatcatatc catgtcgttc cgtattcatg gttgacgcgc aatcagattg
     9541 caacaatcaa gtatgtgatg caacaccccg aagttcgtcc ggatgtatgg tctaagccag
     9601 ggcatgaaga gtcaggttcg gtcattccaa atgttacgcc tcttgataaa cgtgctggta
     9661 tgccaaactg gcaaattatc cattctgctg aagaagttca aaaagcccta gcagaaggtc
     9721 gttttgcagc accagacggc tatattttcg atccacgaga tgttttggca aaagaaactt
     9781 ttgtatggaa agatggctcc tttagcatcc caagagcaga tggcagttca ttgagaacca
     9841 ttaataaatc tgatctatcc caagctgagt ggcaacaagc tcaagagtta ttggcaaaga
     9901 aaaatgctgg tgatgctact gatacggata aacctgaaga aaagcaacag gcagataaga
     9961 gcaatgaaaa ccaacagcca agtgaagcca gtaaagaaga aaaagaatca gatgacttta
    10021 tagacagttt accagactat ggtctagata gagcaaccct agaagatcat atcaatcaat
    10081 tagcacaaaa agctaatatc gatcctaagt atctcatttt ccaaccagaa ggtgtccaat
    10141 tttataataa aaatggtgaa ttggtaactt atgatatcaa gacacttcaa caaataaacc
    10201 cttaaccaaa agaagatctc attgttaaag cactgctttg tcaaagcaag ttacggtgat
    10261 tttgaagtca ttctatgtaa cgagtagtga taaaagttgg ataatagcgg ttttcttttg
    10321 caaagaaatg gtatccatgt tagaatagta aaaaaagagg aggattcttg gactaatgtc
    10381 aaataagtag acagaaaact gtgttatttt attgcgttaa aataattttc ttctttctga
    10441 ttaggggtta gtcctagatt agccgtatgt gggatgtaat tgttataaaa attctcaatg
    10501 tattcaaagc agtctaattg aacctgtttg atattttgat aatgttttcg gttgatttgt
    10561 ctatgcttta aatacttgaa aaatgcttca gttacggcat tatcataagg atatccagga
    10621 ttagaaaaag aatgcatgat attggcactg caccctaata gtgagacgca agaaaaacac
    10681 ttttaggcaa tcagttttct gtactgtaca ggcgactggt cgtttaatct ctgttgaatt
    10741 ctagtttcat tataaaatgt aatgtaattt ttaacaatat ttgttatact atctttgttg
    10801 tattttctcc tattatggaa ataaaaggtt tcagtcttta ggacggtgtg aaaccattca
    10861 atacaggcat tatctgcagg tgttcctttt cgagacattg agcggataat gtctttttcc
    10921 gtgcaagcct ggtagtaagc catagaagta tacactgagc cttggtcact gtgtaagatt
    10981 gctcctttat ttaggcaatt ttaactgatt aagggtgtct agtacaaaat ccgtgtcctg
    11041 acaatctgag atagtgtaag ctataatttc tcggttatag agatccataa ttgatgagag
    11101 atacaattta cagttaccga aatataggta ggtaatatct gttacgagct tttccttagg
    11161 cttatcggca tggaaatccc gactcaattt attatctgtt aaataataag ctttacccaa
    11221 attgggaact ttcttggtac gtgtccgaca aagccagcca ttatttttca tgatacgata
    11281 gactttcttt gtattaacag tcaatccgtg gatttttttg agcaatcgtg taatggtacg
    11341 atagccataa ataaagtgat tctccataca gagctgttca attaattcaa taaggtcatc
    11401 tttttttgcg gcttctcata ctcctttttc caacggtaat aggtcgaccg cttgacctta
    11461 aaacagtcta gaatgaaaac tatcgggtag ttgtttttat agtcttccac aagcttgata
    11521 agacttactt tatcgatttc cttatcaagc ctcgatactt ttttaagagg tcaacctgta
    11581 attgtaattg ttccacttca gacagatgtt ccaagccttt accgtaggta tattgcttgc
    11641 caacaccttg atgaaaacga taaagctcct cgttttcgta ccatttcatc caagtataga
    11701 tttgactatt atttttgatg cctaaagtct ccataataac tctgttagac ttgcctgctt
    11761 tcttcatatc gatgcaagcc agcttagttt cccatgaata tgctttttta accataataa
    11821 aacactcctg tttctagttt actaaatttc aacaggagtg tttttctttt gtctcatttt
    11881 agggattcag tgcctattgt tgtcatcaat tatttttcta aattccccgg acttaaattg
    11941 tgacccttgg tcggaatgaa agagaagtgt tccttcaatc tttcttttat taa
//
LOCUS       DATRMA010000059        11468 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.059, whole genome shotgun sequence.
ACCESSION   DATRMA010000059 DATRMA010000000
VERSION     DATRMA010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11468
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.059"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            246..1052
                     /locus_tag="VBC28_001508"
     CDS             246..1052
                     /locus_tag="VBC28_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEO6815481.1"
                     /translation="MSKKIILGILSLLSVVTLVACGSSDKQLQDKVEKKGKLVLAVSP
                     DYAPFEFKALVNGKDKVVGADIELAQAIADELHVKLEVSPMSFDNVLSSLQTGKADMA
                     ISGLSYTKERAKVYDFSKPYYTTENAVLVRKSDLGKYTSIESLKGKKIAVQKGSIEEG
                     VSKKSFKNSHVVSLTAMGEAISELKSGKVEAVDLEKPVAEGYLAQNPDLVLAKFALKT
                     GEGDAKAVALPKDSGKMLKTVNKVIKRLEKEDKYKVYISDAAKLTGNQVE"
     gene            1135..1638
                     /locus_tag="VBC28_001509"
     CDS             1135..1638
                     /locus_tag="VBC28_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HEO6815482.1"
                     /translation="MVQGSINIEKYKDLALQKQGEHRKFLASLKKKAPKNLDKIVQQI
                     HQEVFQEIDCTACANCCKTLGPLFTEADISRISKHFRMKLSAFEDLFLEVDEDGDKVF
                     KAMPCPFLGDDNLCSIYDVRPKACREFPHTDRKKIYQINNLTIKNTLTCPAAYLFVEK
                     LKSRIGK"
     gene            1742..1918
                     /locus_tag="VBC28_001510"
     CDS             1742..1918
                     /locus_tag="VBC28_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEO6815483.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVTKAGTLQESRKREFYEKPSVK
                     RKRKSEAARKRKKF"
     gene            complement(2015..2392)
                     /gene="mscL"
                     /locus_tag="VBC28_001511"
     CDS             complement(2015..2392)
                     /gene="mscL"
                     /locus_tag="VBC28_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEO6815484.1"
                     /translation="MIKELKEFLFKGNVLDLAVAVILGAAFNAIITSLVKDVITPLIL
                     NPVLKAAGVSNIAQLSWNGVAYGNFLSAVINFLIVGTTLFFIVKAANKVMAKKPAEEE
                     IIEVVEPTQEQLLAEIRDLLANK"
     gene            2516..4390
                     /gene="dnaG"
                     /locus_tag="VBC28_001512"
     CDS             2516..4390
                     /gene="dnaG"
                     /locus_tag="VBC28_001512"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEO6815485.1"
                     /translation="MKKLLKKFTQKRKNDIIKCKTIMGYFCGGHDLAIDKEKISEIKN
                     SVNIVDVIGEVVGLTKTGRNHLGLCPFHKEKTPSFNVIEDRQFFHCFGCGRSGDVFKF
                     VEDYQHISFLDSVQVLAERSGIPLDTNFKGQVPKKPKANQSLLDIHRVASGFYHAYLM
                     TTNDGERARQYLAERGVTEDLIKHFQIGLSPGGQDFLYRRLAKEFDEKTLMSSGLFNY
                     SENSNQFYDSFNNRIMFPLTNDIGEVIAFSGRVWTQEDIDRKQAKYKNSRATPIFNKS
                     YELYHLDKARAVINKAHEVYLMEGFMDVIAAYRAGIENVVASMGTALTNEHVRHLKRF
                     TKKVVLTYDGDRAGQNAIDKSLELLSDMTVDIVRIPNKMDPDEFLQANSAEDFKQLLE
                     NGRISNTEFYIHYLKPENTDNLQSEIAYVEKIAKLIAKSPSITAQNSYITKVAELLPD
                     FDYFQVEQSVNNERLHHRSQQQASSSVQTSATVQLPQTGKLSAITKTEMQLFHRLLNH
                     PYLLNEFRNRDNFYFDTTEIQVLYELLKESGEITSYDLSQESDKVNRTYYIILEEQLP
                     VEVSIGEIEAVEKARDRLLKERDLRKQSQLIRQSSNQGDEEGALAALENLIAQKRNME
                     "
     gene            4398..5507
                     /gene="rpoD"
                     /locus_tag="VBC28_001513"
     CDS             4398..5507
                     /gene="rpoD"
                     /locus_tag="VBC28_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019787874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEO6815486.1"
                     /translation="MAEKKGNTTFNVQVADFIRNHKKQGTAIDDEVTEKLVIPFVLDA
                     DQIDDLLERLTDGGISITDKEGNPSTKYVVEGPKPEELTDEELIGSNSAKVNDPVRMY
                     LKEIGVVPLLTNEEEKELAVAVAEGDLMAKQRLAEANLRLVVSIAKRYVGRGMQFLDL
                     IQEGNMGLMKAVDKFDYSKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINK
                     LVREQRNLLQELGQDPTPEQIAERMDMTPDKVREILKIAQEPVSLETPIGEEDDSHLG
                     DFIEDEVIENPVDYTTRVVLREQLDEVLDTLTDREENVLRLRFGLDDGKMRTLEDVGK
                     VFNVTRERIRQIEAKALRKLRHPSRSKQLKDFMED"
     gene            5616..5957
                     /locus_tag="VBC28_001514"
     CDS             5616..5957
                     /locus_tag="VBC28_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017766829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEO6815487.1"
                     /translation="MSEVKQYSEEEVGKIKDRILEALEMVIDPELGIDIVNLGLIYEI
                     RFEDNGRTEIDMTLTTMGCPLADLLTDQIHDVMKTVPEVTETEVKLVWYPAWSVDKMS
                     RYARIALGIRS"
     gene            6047..6901
                     /gene="rfbD"
                     /locus_tag="VBC28_001515"
     CDS             6047..6901
                     /gene="rfbD"
                     /locus_tag="VBC28_001515"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEO6815488.1"
                     /translation="MILITGANGQLGSELRHLLDERTQEYVAVDVAEMDITNAEMVDK
                     VFEEVKPSLVYHCAAYTAVDAAEDEGKELDFAINVTGTENVAKAAAKHDATLVYISTD
                     YVFDGEKPVGQEWEVDDLPDPKTEYGRTKRMGEELVEKYTSKFYTIRTAWVFGNYGKN
                     FVFTMQNLAKTHKTLTVVNDQHGRPTWTRTLAEFMTYLAENQKDFGYYHLSNDAKEDT
                     TWYDFAVEILKDTDVEVKPVDSSQFPAKAKRPLNSTMSLEKAKATGFVIPTWQDALKE
                     FYKQEVKK"
     gene            7018..8172
                     /locus_tag="VBC28_001516"
     CDS             7018..8172
                     /locus_tag="VBC28_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001154391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1972 domain-containing protein"
                     /protein_id="HEO6815489.1"
                     /translation="MQDVFIIGSRGLPARYGGFETFVSELINHQKSFDIKYHVACLSD
                     KEHHTHFNFADADCFTINPPQLGPARVIAYDIMAINYALDLVKTHDLKEPIFYILGNT
                     IGAFIWHFANKIHKVGGLLYVNPDGLEWKRSKWSRPTQRYLKYAEKCMTKNADLIISD
                     NIGIENYIQSTYSNVKTRFIAYGTEINSRKLSSDDPRVKQLFKKWNIKSKGYYLIVGR
                     FVPENNYETAIREFMASDTKRDLVIICNHQNNPYFEKLSLKTNLQQDKRVKFVGTLYE
                     KDLLDYVRQQAFAYIHGHEVGGTNPGLLEALANTDLNLVLDVDFNKSVAGLSSFYWAK
                     KEGDLAKLINDSDQQQDLSTYGDRAKAIIQENYTWKKIVEEYEDLFLNES"
     gene            8162..9103
                     /locus_tag="VBC28_001517"
     CDS             8162..9103
                     /locus_tag="VBC28_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEO6815490.1"
                     /translation="MKVNILMATYNGEKFLAQQIESIQKQTFKEWNLLIRDDGSSDKT
                     CDIIRNFTAKDSRIRFINENEHHNLGVIKSFFTLVNYEVADFYFFSDQDDVWLPEKLS
                     VSLEAAKHKASDVPLLVYTDLKVVNQELNILQDSMIRAQSHHANTTLLPELTENTVTG
                     GTMMINHALAEKWFTPNDILMHDWFLALLAASLGEIIYLDLPTQLYRQHDNNVLGART
                     MDKRFKILREGPKSIFTRYWKLIHDSQKQASLIVDKYGDIMTANDLELIKCFIKIDKQ
                     PFMTRLRWLWKYGYSKNQFKHQVVFKWLIATNYYNKR"
     gene            9105..9836
                     /locus_tag="VBC28_001518"
     CDS             9105..9836
                     /locus_tag="VBC28_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEO6815491.1"
                     /translation="MMKKILLVIPAYNEEGSIAKTVQTIVDFKASRSLPFELDYIVIN
                     DGSTDGTPELLDRLGLNHIDLVQNLGIGGCVQTGYLYANRNHYDVAVQFDGDGQHDIR
                     SIEDVVMPILNDEADFVIGSRFVDKKHQNFQSTAMRRLGINLISAAIKLTTGHKVYDT
                     TSGYRAANAALIAYLSCHYPVQYPEPESTARILKKGYRLKEVTANMFEREAGTSSISS
                     LKSIFYMTDVLTSIIIAGFIKEDDK"
     gene            9833..10186
                     /locus_tag="VBC28_001519"
     CDS             9833..10186
                     /locus_tag="VBC28_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000050393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2304 domain-containing protein"
                     /protein_id="HEO6815492.1"
                     /translation="MSLALQIEMFILAIVLLYMIIRMVNKNTFSVKRSLPWLLVGLIL
                     VFISVFPGVVSFIAHHLGFALTMNFLLFGAILFLFCLELFDTSSNTKRDEQIKRLIQE
                     VSLLKKEIEDSTLDE"
     gene            10179..>11468
                     /locus_tag="VBC28_001520"
     CDS             10179..>11468
                     /locus_tag="VBC28_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface protein"
                     /protein_id="HEO6815493.1"
                     /translation="MSKKHIFICLFFMLFLVGCQNNKKVKTDKKVDITAYYYHNNTPQ
                     YSVQINAIHLQTALDKVRTGEFESKDFTHYTFDSIKRKYQVSGKKVLMDNNYRFGSGI
                     KNTRQDAIAIVRLLLEKNKDYLIYMNGLEEPSVLYNPENKRYKFTNNKGKAIGNIPVG
                     LTAFENSAETQEYVLKNIQKNETIYLGNTRVDNPRVTVNNKKRDTIGVEYGKRITYRI
                     PIYSKQLTVRVSPNFVVDSTNYNYRLSQAPIIAEEGKEGRLYPIDSSISVDGDKIVLN
                     SSGDETEQKELLQNSLYKLHSIDKLDFDLTNADIKELEITGHVASKRDYNLSVAQEKK
                     GVIETKRSLISVDNQKQDQGIFVEDSKGYIKTPSLSSYGINFAMYDDNQNRLLQGSEY
                     ILGRLNADKKVSLYQPNGFWINTQKTIKSLNKEYLNQN"
BASE COUNT         3976 a         1657 c         2257 g         3578 t
ORIGIN      
        1 tcttactaga cagcaactgt acaaggttct gaaagttagt tgattattaa tgatagttag
       61 cattttttaa gaattggaat acttttaaaa ggtgattgtt ttaattagtt atagttgtta
      121 ttaaaaatcg tctaacagca agcatagtta tcggtgtaat ttactgaatt tttattcaaa
      181 aaaacttgac ttatgtataa agatgattta tacttgttcc tatcataata aaaggaagat
      241 agtttatgtc aaaaaaaata atattaggaa ttttatctct tttatctgtc gttactttgg
      301 tggcgtgtgg ttcatcagac aaacagctac aagataaagt tgagaaaaaa gggaagttag
      361 ttttagcggt gagtccagat tatgctccct ttgagtttaa agcgcttgtt aatgggaaag
      421 ataaagttgt cggcgcagat attgagttag ctcaagctat tgctgatgag cttcatgtca
      481 aactagaggt atctcctatg agttttgaca atgttttatc aagtttacaa acaggtaagg
      541 ctgatatggc tatttctgga ttatcatata ctaaagagcg tgctaaagtc tatgattttt
      601 caaaacctta ttacactaca gaaaatgcgg ttcttgttag gaagtctgat ttaggaaaat
      661 atacttcaat cgaaagcctt aaaggtaaaa aaatagcagt acaaaaagga agtatcgaag
      721 agggagtttc taagaaatca ttcaaaaatt ctcacgtcgt gtcactgaca gcgatgggag
      781 aagcgattag tgagttaaaa tcaggcaagg tagaagctgt tgatttagaa aaacccgtag
      841 ctgaaggtta cttggcccag aatccagacc ttgttcttgc taagtttgcc cttaaaacag
      901 gagaagggga tgctaaggct gtagctttac ctaaagacag tggaaaaatg ttgaagactg
      961 ttaacaaagt gataaagaga cttgaaaaag aagataaata taaagtttat atttcagatg
     1021 cggctaaatt aactggcaat caagttgaat aatattaaaa agcctagtct taggcttttt
     1081 tggatgttat aatatggctt gactaagaat aatagcataa aggcagagga atatatggtt
     1141 cagggttcaa ttaatattga aaaatataaa gatttggcgc tccaaaaaca aggcgagcat
     1201 cgtaaatttt tagcaagttt aaaaaagaaa gctcctaaaa acttagataa aatagtccaa
     1261 cagatacatc aagaggtttt tcaagaaatt gattgtacag catgtgctaa ttgttgtaaa
     1321 actttgggtc ctctatttac agaagctgat atttctagga tttctaaaca tttccggatg
     1381 aagttatcgg cttttgaaga tttattttta gaagtagatg aagatggaga taaagtattt
     1441 aaagcgatgc cttgtccgtt tttaggcgat gataacttat gtagtatata cgatgtcaga
     1501 cctaaagctt gtcgtgaatt tccacatact gatcgtaaga aaatctatca aattaataac
     1561 ttaacgataa aaaatactct tacttgtcca gcggcatacc tttttgtgga aaaattgaaa
     1621 tcacgtatag ggaaataata acccagagaa agtatcataa aaatattgac aatctgcttt
     1681 tatttgggta tcctagtaag tgaacgtgaa aatgttacaa tatcttgagg aggtgaaaca
     1741 tatgtcaaaa acagtagtac gtaaaaacga atcacttgac gatgcacttc gtcgtttcaa
     1801 acgttctgtg actaaagctg gtactcttca agaatcacgt aaacgtgaat tctacgaaaa
     1861 accttctgta aaacgtaaac gtaaatcaga agcagctcgc aaacgtaaaa aattctaatt
     1921 agatagaata tgaaaaaaca gttcagaatt gagctgtttt ttcatatatt ataactataa
     1981 gttaataaaa aaactcggta atccgagtct tttattattt gttagcgagt aagtcgcgga
     2041 tttcagcaag taattgctct tgtgttggtt caacaacttc aataatttct tcttcagcag
     2101 gtttcttagc cataaccttg ttagcagctt taacgatgaa gaataaagta gtaccaacga
     2161 ttaagaaatt aataactgca cttaagaaat taccgtaagc aacaccattc catgaaagct
     2221 gtgcgatatt tgaaacgcca gcagctttta atacaggatt aaggataagc ggtgtaataa
     2281 cgtctttaac aagtgaagtg atgatagcat taaaagctgc acctaaaata acagcaacag
     2341 caaggtctaa aacattaccc ttaaataaaa attcttttag ttctttaatc atgtgaacaa
     2401 gctccttaat attatttaat tcacgttcat tatagcagat ttcttttgag aaatccaagt
     2461 aataggtttc aaattatctg taaattttta tattaaatca gtgaagaaga aatccttgaa
     2521 aaagcttttg aaaaaattta cacagaaaag aaaaaatgat ataatcaaat gtaaaactat
     2581 aatgggttat ttctgtggag gtcatgactt ggcaattgat aaagaaaaaa tttctgaaat
     2641 caagaacagt gttaacattg ttgatgtcat cggtgaagtt gtcggtttga caaaaacagg
     2701 acgcaatcat cttggtttat gcccatttca taaagaaaaa acgccatctt ttaatgttat
     2761 cgaagatagg cagttttttc attgttttgg ttgtggtcgc tctggtgatg tctttaaatt
     2821 tgtggaagat taccaacata ttagtttttt agatagtgta caagttctag ctgaacgttc
     2881 aggaatacct ctagatacta attttaaggg gcaagttcct aaaaaaccta aagctaatca
     2941 gtcgctttta gatattcata gagttgcttc gggattttac catgcctatt taatgacaac
     3001 taatgatggt gaacgcgcta gacaatacct tgccgagcgc ggagtgacag aggacttaat
     3061 taaacatttt caaataggat tatcgccggg agggcaggat tttctatatc gtcgtctggc
     3121 aaaagagttt gatgaaaaaa ctttgatgtc ttctggtttg ttcaattatt cagaaaatag
     3181 caatcagttt tacgattcat ttaataatag aattatgttt ccgttaacta atgatattgg
     3241 tgaagtcatt gcgttttcag gacgagtttg gactcaagag gacattgata gaaaacaagc
     3301 taaatataaa aattctcgtg ccacccctat ttttaataag tcttacgagc tttaccattt
     3361 agataaagcg cgtgctgtaa ttaataaagc acacgaagtt tacctaatgg aaggctttat
     3421 ggatgttatt gcagcttatc gtgcaggaat tgagaatgtt gttgcttcta tggggaccgc
     3481 tttaaccaat gaacatgtta gacaccttaa aagatttact aagaaagttg ttctgactta
     3541 tgatggtgat agagctggtc aaaacgcaat tgacaagtct ttagaactgc tatcagatat
     3601 gactgttgat atcgttagga tacctaataa aatggaccct gatgaatttc tacaagcaaa
     3661 ttcagcagaa gattttaaac aactcttaga aaatgggcgc atcagtaata cagagtttta
     3721 tatccactac ttaaagcctg aaaacaccga taatttacaa tctgaaattg cttatgttga
     3781 aaaaatagcg aaattaattg ctaaatctcc atcgataaca gcacaaaatt catatattac
     3841 aaaagtggct gaactcttgc cagattttga ttattttcaa gtagaacaat cggttaataa
     3901 cgaacgattg catcatagaa gtcaacagca agcttcatct tcagtacaaa caagtgctac
     3961 agtgcaatta ccacagacag gtaagttatc agctatcact aaaacagaaa tgcaattgtt
     4021 tcatcgatta ttgaatcatc cctacctgtt aaatgaattc cgtaataggg ataattttta
     4081 ttttgataca acagaaatac aagttttata tgagttgcta aaagaaagtg gtgagatcac
     4141 gtcttatgat ttatcacaag aaagtgacaa agttaatcgt acttattata taattttaga
     4201 ggaacaatta cctgtagaag tatctattgg agagattgaa gccgtcgaaa aggcacgtga
     4261 ccgtttattg aaagaacgtg atttaagaaa acaaagccag ttaattagac aatctagtaa
     4321 tcaaggtgat gaagaaggag ccttggctgc attagaaaat ttaattgctc aaaaaagaaa
     4381 tatggaatga ggaacctatg gcagagaaaa aaggaaatac aacttttaat gttcaagttg
     4441 cggattttat tcgcaatcat aaaaaacaag gtactgcaat tgatgatgag gtaacagaaa
     4501 aacttgttat tccttttgtt ttagatgcag atcaaattga tgatttgtta gaacgattga
     4561 cagacggtgg catttcaatc acagataaag aaggcaatcc ttcaacaaag tatgtggtag
     4621 aagggccaaa acctgaagaa ttgactgatg aagagctcat aggaagtaat tctgctaaag
     4681 tcaatgaccc agtacgtatg tacttgaagg aaattggtgt tgttcctctt ttaacgaatg
     4741 aagaagaaaa agaattggct gttgcggttg ctgagggaga tttaatggct aaacaacgtt
     4801 tagctgaagc aaaccttcgt cttgttgtct caattgcaaa acgttatgtt ggacgtggaa
     4861 tgcaatttct tgatttaatt caagagggta acatggggct catgaaagct gttgataaat
     4921 ttgattattc aaagggattc aaattttcaa cttatgcaac atggtggatt cgtcaagcta
     4981 taacacgtgc aattgcagat caagcacgta cgattcgtat tcctgttcat atggttgaaa
     5041 cgattaataa attggtccgt gaacaacgta atcttcttca agaacttggt caagatccaa
     5101 ctcctgagca aatcgcagaa cgcatggata tgacaccaga taaagtacgt gaaatcctaa
     5161 aaattgctca agagccggta tcgctagaaa cgccaatcgg tgaggaagat gatagccatt
     5221 tgggtgattt cattgaagac gaagttattg aaaatccagt agattatact acacgtgttg
     5281 ttcttcgtga gcaattagat gaggttttag atactcttac tgaccgtgaa gagaatgttc
     5341 ttcgtttacg ctttggtctt gacgatggta aaatgcgtac gttagaggac gtaggtaaag
     5401 tatttaatgt tactcgtgag cgcattcgtc agattgaagc taaagccctt agaaaacttc
     5461 gccatcctag tcgtagcaag caacttaaag atttcatgga agattgattg cttctcgata
     5521 atcgttttta gaataggaag tttagtttct aataaaaatg atttgcaaag gagttatctt
     5581 aaagacggta ttaatatttt cagagaggta ttataatgtc agaagtaaag caatatagcg
     5641 aagaagaagt tggaaaaata aaagatcgta tcctagaagc tttggagatg gttatcgatc
     5701 ctgaattggg aatagatatt gttaaccttg ggttaattta tgagattcgc tttgaagata
     5761 acggtcgaac tgaaattgat atgactttaa cgactatggg atgcccgtta gcagatttat
     5821 tgacagatca aattcatgat gttatgaaaa cagtcccaga ggtaactgaa accgaggtga
     5881 aattggtatg gtacccagcc tggtctgttg ataagatgag tcgttatgct cgtatcgctt
     5941 tgggaattcg ttcataagat aaagagtaaa gggatgaaat attccctttt ttagtgtaca
     6001 ataaacgaag aattaaaagt taaacttatt tttaaaggag caaggaatga ttttaattac
     6061 aggggcaaac gggcaattag gtagtgaatt acgtcacctt ttagacgaac gaactcaaga
     6121 gtatgttgct gttgatgtag ctgaaatgga tattacaaat gccgagatgg tggacaaagt
     6181 ctttgaagag gtgaagccat ctttagttta ccattgtgca gcttacacag ctgttgatgc
     6241 ggcagaagat gaaggaaaag agctagactt tgcaatcaat gtcactggta cagagaatgt
     6301 cgctaaggct gctgctaaac atgatgcaac acttgtttac atttcgacgg actacgtgtt
     6361 tgatggtgaa aaaccagtgg ggcaagaatg ggaagtagat gatctgcctg atcccaaaac
     6421 agagtacggt cgtacaaaac gtatgggtga agagttagtt gaaaaatata cttcaaaatt
     6481 ctataccatt cgcacagcgt gggtatttgg taactatggt aaaaactttg tctttacgat
     6541 gcaaaacctt gcaaaaacac acaaaactct aactgttgtt aacgaccaac atggacgacc
     6601 aacatggaca cgtactttgg ctgagtttat gacttattta gctgaaaatc aaaaagattt
     6661 tggttactat catttatcaa atgatgctaa agaagataca acatggtatg attttgcggt
     6721 tgaaatctta aaagacacag atgtggaggt taagcctgtt gattctagtc aattcccagc
     6781 aaaagcaaaa cgtcctttaa attcaactat gagtttggaa aaagcgaaag caacaggatt
     6841 tgtgattcct acttggcaag atgctttaaa agagttctac aagcaggaag ttaaaaaata
     6901 agatcaaaac aaagagtagt aaggctagag aatcctttta gcttactgct ctttttagct
     6961 aacttttcta atttatggta taattgtatg gattgtttag ctagaatgga gaagatgatg
     7021 caagatgttt tcattatagg aagtagaggg ttgccagctc gttacggtgg ttttgaaact
     7081 tttgtttcag aattgattaa tcatcaaaaa agtttcgaca taaaatacca tgttgcatgc
     7141 cttagtgata aagaacatca tactcatttt aactttgctg acgctgattg ttttactata
     7201 aatcctcccc aattagggcc agcacgtgtg attgcttatg atattatggc cattaattat
     7261 gcccttgact tggttaagac acatgattta aaagagccta ttttttatat tttaggaaat
     7321 acaattggtg cctttatttg gcattttgcc aataaaatac ataaagtcgg tggcttattg
     7381 tatgttaatc cggatggttt agagtggaag cgatcaaagt ggtctcgtcc cacacagcgt
     7441 tatttaaaat acgccgaaaa atgtatgact aaaaatgcag acctaattat ttctgataat
     7501 attggtattg aaaattacat tcaatctacc tactctaatg tgaagacaag gttcattgct
     7561 tacggtacag agattaattc taggaaatta tcgtcagatg atccacgtgt caaacagttg
     7621 tttaaaaaat ggaatattaa gtctaagggt tactatctaa tcgttggtcg atttgtccct
     7681 gaaaacaatt atgaaacggc tattagggag ttcatggctt cagatactaa gcgtgattta
     7741 gttattatct gtaaccatca aaataacccc tactttgaaa agttgtcctt aaagacaaac
     7801 cttcaacaag ataaaagagt taagtttgta ggtacgctct atgaaaaaga tctgctggat
     7861 tatgttcgtc aacaagcctt tgcttatatt catgggcatg aagttggcgg tactaatcca
     7921 ggactgcttg aggctttagc taatactgat ttgaatcttg ttctagatgt tgatttcaac
     7981 aaatcagtag caggtctctc aagtttttac tgggctaaaa aagaggggga tttagctaag
     8041 cttattaacg atagtgatca acaacaggat ttatctactt acggtgatcg agcaaaagcc
     8101 attattcaag aaaactatac ttggaaaaaa attgttgaag aatatgagga tttgttttta
     8161 aatgaaagtt aatattttaa tggccaccta caatggagag aagtttctgg cgcagcaaat
     8221 cgaaagtatc caaaagcaaa cctttaaaga gtggaacctc ctaatcagag atgatggttc
     8281 aagtgacaag acatgtgata ttattagaaa ctttactgct aaagattctc gaattcgttt
     8341 cattaatgaa aatgagcacc ataatttagg agttattaaa tcatttttta cgctagttaa
     8401 ttacgaagtt gcagatttct atttttttag tgatcaagat gatgtatggt tgccagaaaa
     8461 gttatctgta agtttagagg cagcaaagca taaagcatct gatgtaccat tacttgttta
     8521 tactgatttg aaagttgtta atcaagaatt aaatatttta caagacagca tgattcgggc
     8581 tcaatcgcac catgccaata cgacattatt accagaacta actgagaata ctgttactgg
     8641 tgggactatg atgataaatc atgcattagc tgagaagtgg tttactccta atgatatctt
     8701 aatgcatgat tggtttttag cactcttagc agcaagttta ggagaaatca tttatttgga
     8761 tttaccaact caactatacc gtcaacatga caataatgtt ttgggggcga ggacaatgga
     8821 taagagattt aagatacttc gtgaaggtcc taaatctata tttacaaggt attggaaact
     8881 tatccatgac tcacaaaagc aagcttcctt aattgttgat aagtatggtg atattatgac
     8941 agctaatgat cttgagctca ttaaatgttt tataaaaatt gacaaacagc cttttatgac
     9001 aagattaagg tggttatgga aatatggtta tagcaaaaat caatttaagc atcaagttgt
     9061 gtttaaatgg ttgattgcaa caaattatta caataagaga taagatgatg aaaaaaattt
     9121 tactagtgat acctgcttat aatgaagaag gtagtattgc aaagacagtc cagactattg
     9181 tagattttaa ggcgtctcgc tcactacctt ttgagctgga ttatattgtt attaacgatg
     9241 ggtcaaccga tggtactcca gagttattag atagattggg tttaaatcat attgaccttg
     9301 ttcaaaatct aggtattggt ggctgtgtgc agacaggata cttatacgct aatcgtaatc
     9361 attacgatgt tgccgttcaa tttgatggtg atggtcaaca tgatattaga agcattgaag
     9421 atgttgttat gccaatctta aatgacgaag cagattttgt tattggttct cgctttgttg
     9481 ataaaaaaca tcaaaatttt caatcgacag caatgaggcg tcttggcata aatttaatat
     9541 ctgctgctat aaaattaacg acgggacaca aagtctatga tacaacaagt ggttataggg
     9601 cagccaacgc ggcgctaata gcttatttat cgtgtcatta tccagtacaa tacccagagc
     9661 ctgaatcaac tgctcggatt ttgaaaaaag gctatcgttt gaaggaagtc acagctaata
     9721 tgtttgaacg tgaggcaggt acttcaagta taagttccct aaaatcgatt ttttatatga
     9781 cggatgtttt aacatcaatt attattgcag ggtttattaa ggaggatgat aaatgagttt
     9841 agcattgcaa attgaaatgt ttattttagc cattgtccta ctttatatga ttataagaat
     9901 ggttaataaa aacacatttt ctgttaaacg ttctttacct tggctattag ttggtttaat
     9961 tctagtcttt attagtgtat ttcctggggt ggtctcattt atcgctcatc accttggatt
    10021 cgctttaacg atgaatttct tgttatttgg tgctatttta tttctgtttt gtttagaatt
    10081 atttgatact agtagcaata ccaaaagaga cgagcaaatt aaacgcctga ttcaagaggt
    10141 ttctttgttg aaaaaagaga tagaggatag cactttagat gagtaaaaaa catattttta
    10201 tttgtctgtt tttcatgctt tttttagtag gttgccaaaa taataaaaaa gtaaaaacag
    10261 ataaaaaagt agatattaca gcttattatt atcataataa taccccgcaa tacagtgttc
    10321 agattaatgc tattcattta caaacagcat tggataaagt gaggacgggg gagtttgaga
    10381 gcaaagactt tacacactat acttttgata gtatcaagcg gaagtaccaa gttagtggta
    10441 aaaaagtcct tatggataat aactatagat ttggttctgg tataaaaaat actagacagg
    10501 atgctattgc tattgtaagg ttactgcttg aaaagaataa agattattta atttacatga
    10561 atggcttaga agagccaagt gttttatata atccagaaaa taaacgttat aagttcacaa
    10621 ataacaaagg aaaagcaatt ggtaatattc cagttggttt aactgctttt gaaaacagcg
    10681 cagaaactca agaatatgtg ttgaaaaata ttcagaaaaa cgaaacgatt tatcttggta
    10741 atacccgagt agataatcct cgagtaactg ttaataacaa aaaaagagat actataggag
    10801 ttgaatacgg taaacgtatt acttatagga ttccaattta ttcaaagcag ctgactgtca
    10861 gagttagccc taattttgta gtagatagta ctaattataa ttaccgactt tctcaagcgc
    10921 ctattattgc ggaagaaggt aaagagggac gtttgtatcc aatagatagc agtattagtg
    10981 tcgatggtga taaaattgtt ttaaatagta gtggtgatga aacagagcaa aaagaattat
    11041 tacaaaatag cctgtataaa cttcactcta ttgataagtt agactttgat ttgacaaatg
    11101 ctgatattaa agagttagag attacaggac atgttgcttc aaaaagagat tataatttgt
    11161 ctgttgctca ggaaaagaaa ggcgtcatag agactaaacg gagtctaatt tctgttgata
    11221 atcaaaaaca agatcaaggg atttttgtcg aggattctaa aggctatata aagacaccta
    11281 gtttgagcag ttatggtata aattttgcca tgtatgacga caatcaaaat agattattac
    11341 aaggttcaga gtatatttta ggaagattga atgcagataa aaaagtatct ctatatcaac
    11401 ctaatggctt ttggataaat actcaaaaaa cgattaagag tttaaataaa gaatatctta
    11461 accaaaat
//
LOCUS       DATRMA010000060        11177 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.060, whole genome shotgun sequence.
ACCESSION   DATRMA010000060 DATRMA010000000
VERSION     DATRMA010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11177
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.060"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            139..592
                     /locus_tag="VBC28_001521"
                     /pseudo
     CDS             139..592
                     /locus_tag="VBC28_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649420.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
     gene            complement(637..2109)
                     /locus_tag="VBC28_001522"
     CDS             complement(637..2109)
                     /locus_tag="VBC28_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO6815494.1"
                     /translation="MEKTKSFFGHPRGLSTLFFTEMWERFSYYGMRAILLYYMYYSVS
                     QGGLGMDKTVAASIMAVYGSLVYLSSVIGGFVSDRILGSRKTVLYGGILIMLGHIALA
                     TPFGQTALFISIALIILGTGLLKPNVSEMVGNLYGENDSRRDAGFSIFVFGINLGAFI
                     SPIVVGYLGQEVNFHLGFSLAAIGMFFGLLQYTLDGKKYLTEESLRPNDPLSPEEKSS
                     LYKKVGLILIGIVIVLILLHLMHMLTIEVIIDIFSIIAIAIPIIYFIKILSSKKISSV
                     ERSRVWAYIPLFIASILFWSIEEQGSVVLALFADEQTKLYLNFFGHHINFPSSYFQSM
                     NPLFIMLYVPFFAWLWAKWGSKQPSSPKKFAYGLFFAGASFLWMMLPGLLFGVNAKVS
                     PLWLTMSWAIVIVGEMLISPVGLSATSKLAPKAFQAQMMSIWFLSNAAAQAINAQIVK
                     LYTPDTQTLYYGVVGGITVVFGFILLFYVPRIEKLMSGVK"
     gene            complement(2340..3176)
                     /locus_tag="VBC28_001523"
     CDS             complement(2340..3176)
                     /locus_tag="VBC28_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEO6815495.1"
                     /translation="MNKKKRLNLTFVYILLIVLSIMWLFPIVWVVLTSFRGEGSAFVN
                     YFIPKTWTLDNYAKLFTQNTFPFGQWFLNTLFVATCTCILSTLITVAMAYSLSRIKFK
                     HRNGFLKLALVLNMFPGFMSMIAVYYILKALNLDQTLTALIFVYSAGAALTFYIAKGF
                     FDTIPYSLDESAMIDGATRLDIFLKITLPLSKPIIVYTALIAFMGPWMDFIFAKVILG
                     DATSKYTVAIGLFSMLQQDTINQWFMSFTAGSVIIAIPITILFMFMQKYYVEGITGGS
                     VK"
     gene            complement(3176..4546)
                     /locus_tag="VBC28_001524"
     CDS             complement(3176..4546)
                     /locus_tag="VBC28_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEO6815496.1"
                     /translation="MNKKLISLDGMSLKEVFQKGDLATKLSFLIMGLANLKNKQIVKG
                     LLFLISEILFLITFVYQVIPAVKGLISLGTQEQGMTTKTVDGIKIQVATQGDNSMLML
                     IFGLASLIFCCVFAYIYWSNIKSAAHLLTLKEEGREIPSFKKDIKSLTDGRFHMTLMS
                     IPLIGVLLFTILPLVYMICLAFTNYDHNHLPPKSLFDWVGFANFGNIFSGRMASTFFP
                     VLSWTLIWAVFATVTNFFFGIILALLINTKGLKFKKMWRTIFVITMAVPQFISLLIMR
                     NLLSDAGPVNALLIKWGLISSAHPLPFLSDPVWAKFSIIFVNMWVGIPVTMLVATGII
                     MNLPAEQIEAAEIDGANKFQVFQSITFPQILLIMTPTLIQQFIGNINNFNVIYLLTQG
                     GPTNSTYYQAGSTDLLVTWLYNLTVTAADYNLASVVGILIFILSAVFSLLAYTRTNSY
                     KEGAAK"
     gene            complement(4644..5891)
                     /locus_tag="VBC28_001525"
     CDS             complement(4644..5891)
                     /locus_tag="VBC28_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEO6815497.1"
                     /translation="MEKNTWKKLLVSTAALSVVAGGAIAATHSNSVDAASKTTIKLWV
                     PTDSKASYKAIVKKFEKENKGVTVKMIESNDSKAQENVKKDPSKAADVFSLPHDQLGQ
                     LVESGVIQEIPEQYSKEIAKNDTKQSLTGAQYKGKTYAFPFGIESQVLYYNKTKLTAD
                     DVKSYETITSKGKFGQQLKAANSYVTGPLFLSVGDTLFGKSGEDAKGTNWGNEAGVSV
                     LKWIADQKKNDGFVNLTAENTMSKFGDGSVHAFESGPWDYDAAKKAVGEDKIGVAVYP
                     TMKIGDKEVQQKAFLGVKLYAVNQAPAGSNTKRISASYKLAAYLTNAESQKIQFEKRH
                     IVPANSSIQSSDSVQKDELAKAVIEMGSSDKYTTVMPKLSQMSTFWTESAAILSDTYS
                     GKIKSSDYLKRLKQFDKDIAKTK"
     gene            6147..7175
                     /locus_tag="VBC28_001526"
     CDS             6147..7175
                     /locus_tag="VBC28_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEO6815498.1"
                     /translation="MVTIKDVAAKAGVNPSTVSRVLKDNASISSKTKERVKKAMEELG
                     YVPNVAAQMLASGLTQNIGVIFPPLVLPDRLSEPFFMEILSTSTNEAKEHGFTVSIAT
                     SMSSEELLEQVKLMHLQKRVDGFIVLYSDQKDPIREYLLKYKLPFVVVGAANGYENEI
                     TYIDNDNQLMGKSAIDYLYKKGHKSIQFVTDDLNSEVSEERYLGYFKGARKLGLNQKP
                     ALLFDRGNPQVLEEFINRVKEEETTALIVIGDTVSVRVMQFLSFYKLKVPDDISIMTF
                     NNSLFSHLIHPYLSTFDINVNNLGRTSVRRLIDIIKSPDKVFSETIIVPFTLEERESV
                     RDISKNNN"
     gene            7296..8792
                     /gene="malQ"
                     /locus_tag="VBC28_001527"
     CDS             7296..8792
                     /gene="malQ"
                     /locus_tag="VBC28_001527"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEO6815499.1"
                     /translation="MKKRASGVLMHITSLPGDLGIGTFGREAYAFVDFLVETDQKFWQ
                     ILPLTTTSFGDSPYQSFSAVAGNTHLIDFDLLTLEGFISKDDYQNISFGQDPEVVDYA
                     GLFEKRRPVLEKAVKNFLQEERATRMLSDFLQEEKWVTDFAEFMAIKEHFGNKALQEW
                     DDKAIIRREEEALAGYRQKLSEVIKYHEVTQYFFYKQWFELKEYANDKGIQIIGDMPI
                     YVSADSVEVWTMPELFKLDRDKQPLAIAGVPADDFSDDGQLWGNPIYNWDYHKESDFD
                     WWIYRIQSGVKMYDYLRIDHFKGFSDYWEIRGDYQTANDGSWQPAPGPELFATIKEKL
                     GDLPIIAENLGYIDERAERLLAGTGFPGMKIMEFGFYDTTGNSIDIPHNYTENTIAYA
                     GTHDNEVINGWFENLTVEQKAYAENYMRRLPNEPITETVLRTLYATVSQTTITCMQDL
                     LDKPADSRMNMPNTVGGNWQWRMRKEDLTENRKAFLKEITTIYNRGNK"
     gene            8804..11068
                     /gene="glgP"
                     /locus_tag="VBC28_001528"
     CDS             8804..11068
                     /gene="glgP"
                     /locus_tag="VBC28_001528"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000197732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan family
                     phosphorylase"
                     /protein_id="HEO6815500.1"
                     /translation="MTRNFTTYVGQQGKVLSELTNEEIYVELLNFVKEEAAAKSKNSS
                     QRKVYYISAEFLIGKLLSNNLINLGIYKDVKKELELVGKSIAEIEDVEPEPSLGNGGL
                     GRLASCFIDSISSLGINGEGVGLNYHCGLFKQVFRNNQQEAEANYWIENNSWLVPTDI
                     SYDVPFRDFTLKSRLDRIDVLGYKKDTKNYLNLFDIDGLDYNLIEKGITFDKTEIKKN
                     LTLFLYPDDSDKNGELLRIYQQYFMVSNAAQLLIDEAIERGSNLHDLAEYAYVQINDT
                     HPSMVIPELIRLLTEKHGFEFDEAVSVVRNMVGYTNHTILAEALEKWPLEYLNEVVPH
                     LVTIIKKLDQMIREEQTNPEVQIIDEAGRVHMAHMDIHFSTSVNGVAALHTEILKNSE
                     LKVFYDIYPDKFNNKTNGITFRRWLEFANQDLADYLKELIGDSYLTDATQLEKLLTYA
                     DSNEVHDKLAAIKFKNKLALKRYLKENKGIELDEYSIIDTQIKRFHEYKRQQMNALYV
                     IHKYLEIKRGHFPSRKLTVIFGGKAAPAYTIAQDIIHLILCLSELINNDPEVNKYLNV
                     HLVENYNVTVAEKLIPATDISEQISLASKEASGTGNMKFMLNGALTLGTMDGANVEIA
                     ELAGKENIYTFGKDSDTIINLYETSGYRSKDYYDKDKVIREAVDFIISDDIVSLGNAE
                     RLKRLHDELVGKDWFMTLIDLKEYIAVKEQVLADYEDYESWNKKVIHNIAKAGFFSSD
                     RTIEQYNQDIWHSN"
BASE COUNT         3819 a         1779 c         2097 g         3482 t
ORIGIN      
        1 gttaatcatg tctattatcg gaattattcg atttttctta acctacagta gttaagttac
       61 tatcctttat tttgtagaaa aattgataaa taaatggtat tgtaactcta tcactaaatt
      121 tatatactag aggaaaaaat gtcacgcagt caaaaagtta ttttaactaa tatgtgtctt
      181 attgaagata ataaaggacg tattgtgatg caaatccgtg atcctaaacg ttattcttgg
      241 tcaggagctg ctttaccagg cggccatatt gaagaaggag aatgctctcg ttcgagaagt
      301 tcacgaggag acaggtttaa caataagtaa tcccaaatta gttggtatga agcattggta
      361 cacagatgaa ggcatccgct atttggtatt cctttataaa acatatgatt tttcaggtga
      421 attacaatcg tcagatgaag gtgatgttgt atggattgaa cgttcacaac ttgataagat
      481 tgaactagca tatgatatgc ttaacctttt gagagtattt gacgaagatg acatctcaga
      541 gatgttttat agtaagcgac ttgaagatgg ttttataaga aaattttatt aaacaaaaaa
      601 gaactaggaa tgtaacctag ttctttttag atgtctttat ttaactccag acattagttt
      661 ttcaatgcgt ggaacataaa ataagaggat aaatccaaat acaactgtta taccaccaac
      721 aacaccataa taaagagttt gagtatcagg tgtgtacaat ttaacgattt gagcgttaat
      781 agcttgtgct gcagcattac ttaagaacca gatgctcatc atttgagctt ggaatgcttt
      841 aggtgcgagc ttactagttg ctgataatcc aactggtgag attagcattt ccccaacgat
      901 gacaatagcc caactcattg ttaaccaaag agggcttact ttagcgttaa ctccaaagag
      961 taaacctggt agcatcatcc ataagaatga agctccagca aagaaaagtc catacgcaaa
     1021 ttttttaggt gatgaaggtt gcttacttcc ccatttagcc cataaccaag caaagaatgg
     1081 tacataaagc ataatgaaga gagggttcat actttggaaa taacttgatg ggaaattaat
     1141 atgatgccca aagaagttaa ggtaaagttt tgtttgttca tctgcaaata aggctaagac
     1201 aactgaacct tgttcttcaa ttgaccaaaa tagaattgag gcgataaaga gagggatata
     1261 tgcccacact cgagaacgct caacagaaga aatcttttta ctacttaaaa tcttgataaa
     1321 ataaataatt gggatggcga ttgcaataat actaaaaata tcgataatta cttcaattgt
     1381 tagcatatgc atcaagtgaa gtagaataag tacaataaca ataccaataa ggataagccc
     1441 aactttttta tatagagagg acttttcttc aggacttaaa ggatcgtttg gtctgagact
     1501 ctcttcagtc aaatattttt ttccatctaa ggtatattgg aggagaccaa agaacatacc
     1561 aatagcagca agtgagaaac caagatggaa atttacttct tgtccgaggt accccactac
     1621 aatgggtgaa ataaaagcac caaggttaat accaaaaaca aagatactaa aaccagcatc
     1681 acgacgagaa tcattttctc catataaatt accaaccatc tctgatacat ttggttttaa
     1741 tagtccagta ccaaggataa ttagagcaat cgaaatgaat agagctgttt gaccaaatgg
     1801 tgtagctaga gcaatatgac ctagcatgat tagaatacca ccatacagaa cagttttacg
     1861 actacctaga atacggtcgc tgacaaaacc accaattact gatgagagat aaaccagtga
     1921 accatagaca gccatgattg atgcagcgac agtcttgtcc ataccaaggc caccttgaga
     1981 aacactatag tacatatagt acagcaaaat agcacgcata ccatagtatg agaatctttc
     2041 ccacatttca gtaaagaaaa gagtggacaa accacgagga tgtccaaaga atgattttgt
     2101 tttttccatg aaaaacgctc cttttgtaga taacactatt aataatatag taaatagaag
     2161 caactgtcaa attacttagc actatcaagt tatttttttg tagaggttaa cacccttggt
     2221 aaaccaagtt ttttaactat ctagcttaga aaaaggcttg aattcaatag aacccaagcc
     2281 ttttttagaa aagaaaccac agtgaaataa aaatgtcgtg tctttaaatt atctgaagat
     2341 tattttacag atccacctgt aataccttca acatagtatt tttgcatgaa catgaaaagt
     2401 attgtgattg gaattgcaat aataacagat cctgctgtaa atgacatgaa ccactggttg
     2461 atggtatctt gttgcaacat tgaaaagaga ccaatagcga ctgtgtactt gctcgtagca
     2521 tcacctaaaa taacttttgc aaagataaag tccatccaag ggcccataaa agcgattaat
     2581 gcagtataaa cgataatggg ttttgacaat ggtagggtga ttttaaggaa aatatccaaa
     2641 cgggtcgccc catcaatcat agcagactca tccaaagagt atggaatagt atcaaagaaa
     2701 ccttttgcga tgtagaaggt taatgctgct cctgcagaat agacaaatat taaagcggtt
     2761 aatgtttggt ctagatttag agcttttagg atgtagtaaa ctgcaatcat tgacatgaac
     2821 ccaggaaaca tattcaagac taaagctagc tttaggaaac cattacggtg tttgaatttg
     2881 attctgctga gagaataagc cattgctaca gtgattaaag ttgatagtat acaagtacat
     2941 gtagcgacaa ataatgtatt taagaaccat tgaccaaatg ggaatgtgtt ttgggtgaag
     3001 agtttagcgt agttgtctaa tgtccaagtt ttaggaataa agtagttgac aaaggcactt
     3061 ccttcaccac ggaagcttgt gagaacgacc caaacaatgg gaaagagcca cataattgat
     3121 agtactatca ataaaatata aacaaaagta aggttaagac gttttttctt attcattatt
     3181 tagctgcccc ttccttgtaa gaatttgttc ttgtgtaagc tagtaaacta aatactgctg
     3241 aaagaataaa gattaagata ccaacaacag atgctaagtt ataatctgca gcagttactg
     3301 tcaaattgta tagccaagtg acaagtaaat cggtgctacc tgcttggtag tatgttgagt
     3361 ttgttggtcc accttgagtg agtaggtaga taacgttgaa gttgttaata tttccgataa
     3421 attgttgaat taaagtagga gtcataatca acaaaatttg tgggaaagta attgattgaa
     3481 agacttggaa cttattcgcc ccatcaattt cagcagcttc aatttgttct gcaggtaaat
     3541 tcatgatgat accagtagca acaagcattg ttacaggaat accaacccac atattaacaa
     3601 agatgattga gaatttcgcc cagaccggat cagaaaggaa tggaagggga tgtgctgaag
     3661 aaataagtcc ccattttatt aacaaggcgt taaccggtcc agcgtcactt aagaggtttc
     3721 tcatgattaa gagagagata aattgaggga cagccatggt aataacaaaa atagtacgcc
     3781 acatcttttt gaatttcaaa ccttttgtat taattagtaa ggcaagaata ataccaaaga
     3841 agaaattcgt tacagtagcg aaaacagccc aaattaatgt ccaagataga acagggaaga
     3901 aagtactggc catacgcccg ctaaagatat taccgaagtt agcaaaacca acccagtcaa
     3961 ataaactttt tggcggtaag tggttatgat catagttagt aaatgccaaa caaatcatat
     4021 aaactaacgg aaggattgta aatagaagca cccctattaa aggaatagac attaaagtca
     4081 tatggaagcg tccatctgtc aagcttttaa tatctttttt gaaggatgga atttctcttc
     4141 cttcttcttt caaggtcaat aaatgtgctg cgcttttaat gtttgaccag taaatatagg
     4201 caaatacgca acagaagata agcgaagcta atccaaaaat tagcattaac atagagttat
     4261 caccttgtgt cgcaacttgg attttaatac catccacagt tttagttgtc ataccttgtt
     4321 cttgggtccc taaggaaata agacctttta cagctgggat aacttggtaa acaaaggtaa
     4381 ttaaaaacag aatttcactg attaaaaata aaagtccctt gacaatttgt ttatttttga
     4441 ggttagcaag tcccataatt aagaatgata gtttagttgc taaatcacct ttttgaaaga
     4501 cctcttttag ggacatacca tcaagtgaga tcagtttttt attcataatg gtctccttta
     4561 attgttattt tacaaaataa gagatgggaa gcactctcaa ttgaaaggaa gagtactcac
     4621 ctcatctcat tactctagac gtactatttt gttttagcga tgtctttatc gaattgtttt
     4681 agacgtttaa ggtaatcgct agatttgatt ttaccactgt aagtatcgct aagaatagca
     4741 gcactttctg tccagaaggt tgacatttga ctcaacttag gcataaccgt tgtatattta
     4801 tctgagctac ccatttcgat aactgctttt gcaagttcat ctttttggac gctatcagaa
     4861 gattgaattg atgagttagc aggaacgata tgacgttttt cgaattgaat tttttgactt
     4921 tcagcattag ttagatatgc agcgagtttg tagctagctg agattcgttt agtgtttgaa
     4981 ccagcaggtg cttggttaac ggcataaagt ttaacgccca agaatgcttt ttgttgaact
     5041 tctttgtcac cgattttcat tgttgggtaa acagcaacac cgattttatc ttcaccgaca
     5101 gcttttttag cagcgtcgta atcccatggt ccactttcaa aagcatgaac agaaccatcg
     5161 ccaaatttag acattgtatt ttcagctgtc aagttgacaa aaccatcatt tttcttttga
     5221 tctgcaatcc atttaaggac agaaacacct gcttcattac cccagttagt gcctttagca
     5281 tcttcaccag atttaccaaa taaagtgtcg cctacagaaa ggaaaagagg acctgttaca
     5341 tatgagttag ctgctttaag ctgttgaccg aatttccctt tgcttgtaat tgtttcgtat
     5401 gatttaacgt cgtcagcagt taactttgtt ttattataat aaagaacttg agattcaata
     5461 ccaaatggga atgcataagt tttcccttta tattgtgcac cagtaagtga ttgtttagtg
     5521 tcgtttttag caatttcttt tgagtattgc tctggaattt cttggataac accagattct
     5581 actaattgac caagttggtc atgtggaagt gagaatacat cggctgcctt gcttgggtct
     5641 ttttttacgt tttcttgagc tttggagtca ttagactcaa tcatttttac agtaacgcct
     5701 ttgttttcct tctcgaattt tttaacaatt gctttataag acgcttttga atctgttggg
     5761 acccaaagtt tgatagttgt ttttgaagca gcatcaactg agttagagtg agtagcagca
     5821 attgctcctc ctgcaactac tgaaagagca gcagtactaa caagtaattt tttccaagta
     5881 tttttttcca ttttggttct cctcctagag aatattttaa ttacacctat attatgcaac
     5941 cgtttgcaaa aaaagtcaag tcttttttta aaaaaaataa gaaaaataaa aaaataatat
     6001 caattaatac taaatgagaa gattatttta gtgagagtat tacatttgca gagaaggcaa
     6061 acgtttgttt ttttcttaga ttagagtata atagacaaag gactatacca aaaggtataa
     6121 aatgattaga atgaagaggt cgtaggatgg taactataaa agatgttgct gccaaagctg
     6181 gtgttaatcc atcgacagta agtcgtgtac taaaagataa tgcatctatt tcaagtaaga
     6241 cgaaagagcg cgtgaaaaaa gctatggaag agctgggcta tgttcctaat gttgcagcac
     6301 aaatgttggc tagtggttta acgcaaaata ttggagttat ttttcctcca ctggttttac
     6361 cagaccgctt aagtgaaccc tttttcatgg agattctttc tacatcaact aatgaagcaa
     6421 aagagcatgg gtttacagtt tctattgcaa caagtatgtc ttctgaggag ttgttagaac
     6481 aagtaaaatt aatgcatcta caaaaacgag tggatggttt tattgttctc tattcggatc
     6541 aaaaagatcc tatcagagag tatttattaa aatataaact gccctttgtt gttgttggtg
     6601 ctgctaatgg ctatgaaaat gagattactt atattgataa tgataatcag ctgatgggga
     6661 aatctgccat tgattatctc tataaaaaag gacataaaag catccagttt gttacagatg
     6721 atttaaattc agaagtatca gaagagcgct atttagggta ttttaaaggt gctagaaagt
     6781 taggtttaaa ccaaaagcca gctcttttat ttgacagagg caatccgcaa gttttagagg
     6841 aatttataaa tagagtcaaa gaagaagaaa caactgcttt aattgttatt ggtgatactg
     6901 tctctgttag agtaatgcaa tttctttctt tctataaatt aaaagttcca gatgacattt
     6961 ctatcatgac ctttaataat tctctcttct cacatctgat tcatccttac ctaagcacat
     7021 ttgatattaa tgttaataat ctaggaagga caagcgttcg tcgtctgatt gacatcatta
     7081 aatctccaga caaagtcttt agtgagacta ttattgtgcc ttttacttta gaagagcgtg
     7141 aaagtgtccg agatatttct aaaaataata attaagctat gatataaaga cttctatcaa
     7201 ttttcgatag aagttttttt aaattatgct tgacttatgc aatcgcttgc gttaagatgg
     7261 aggagtaaag taagtaatcg aaaaggagaa tcaatatgaa aaaacgtgca agtggtgtct
     7321 taatgcacat cacttctcta ccaggtgatt taggtattgg aacatttgga cgagaagcct
     7381 acgcttttgt tgattttttg gtcgaaactg atcaaaaatt ttggcaaatt cttcccttaa
     7441 caacaacaag cttcggggac tctccttacc agtctttttc tgcagtagct ggtaatacac
     7501 atttaattga ttttgatttg ctaacgcttg aaggtttcat ttcgaaagat gactaccaaa
     7561 atattagttt tggacaggat ccagaagttg ttgattatgc tggtctgttt gaaaaacgcc
     7621 gtccagtttt agaaaaagca gttaaaaatt tcttgcaaga agagagagct acgagaatgc
     7681 tatctgattt cttgcaagaa gaaaaatggg taactgattt tgctgaattt atggcgatca
     7741 aagaacattt tggtaataag gcgcttcaag aatgggatga caaggctatt atacgccgcg
     7801 aagaagaagc cttagcagga tatcgtcaaa agcttagtga agtgataaaa tatcatgaag
     7861 taacgcaata tttcttttac aaacaatggt ttgagttaaa agaatatgct aatgataaag
     7921 ggattcaaat tatcggtgat atgccaatct acgtttctgc cgatagtgta gaagtttgga
     7981 caatgcctga actgtttaaa ttagatcgcg acaaacaacc acttgctatt gctggtgtac
     8041 cagcggatga tttttctgac gatggtcagc tttggggcaa tcctatttac aattgggatt
     8101 accacaaaga gtcagatttt gactggtgga tttatcgtat tcaatctggt gttaagatgt
     8161 atgactattt gcgtattgat cattttaaag gtttttcaga ttattgggaa attcgaggag
     8221 attatcaaac agctaatgat ggctcatggc aaccagctcc cggtccagaa ttatttgcaa
     8281 ctatcaaaga aaaattaggt gatttaccta ttattgctga gaatttaggc tatattgatg
     8341 agagagcaga gagattactg gctggaacag ggttcccagg tatgaaaatc atggaattcg
     8401 gtttttatga tacaacagga aatagtattg atattcctca taactacaca gaaaatacaa
     8461 ttgcatatgc tggaactcat gataacgaag ttattaatgg ctggtttgaa aatcttacag
     8521 tcgaacagaa agcttatgca gaaaattaca tgcgtcgttt gccaaatgag cccattactg
     8581 agactgtact gcgtaccttg tatgcaaccg ttagtcaaac aacgattaca tgtatgcaag
     8641 atctactaga taaacctgca gatagccgta tgaacatgcc aaataccgtt ggaggtaact
     8701 ggcaatggcg tatgagaaaa gaagacttaa cagaaaatcg caaagcattc ttaaaagaga
     8761 ttacaactat ttataataga gggaataaat aaggaatatt atcatgacaa gaaattttac
     8821 aacttacgtt ggacaacaag gaaaagtatt atcagaatta acaaatgaag agatttacgt
     8881 tgaattgtta aattttgtaa aagaagaagc tgccgctaaa tcaaaaaata gctctcaacg
     8941 taaggtatac tatatttcag ctgagtttct aattggtaaa ttactttcaa ataatttgat
     9001 taacttaggg atttataaag atgtcaaaaa agagttagaa cttgtaggta aatcaattgc
     9061 tgaaatagaa gatgtagagc cagaaccatc acttggtaat ggtggtttag ggcgtttagc
     9121 atcatgtttt attgactcga tttcttcttt aggtattaat ggtgaaggtg ttggtttaaa
     9181 ctatcactgt gggcttttca aacaagtatt tcgcaacaat caacaagaag cagaagctaa
     9241 ctactggatt gagaataatt cttggttagt accaacagat atttcttatg atgttccttt
     9301 ccgtgacttt actttaaaat cacgcctaga tagaattgat gtattaggtt ataagaaaga
     9361 tactaaaaat tatttgaacc tttttgatat tgatggactt gattataacc ttattgaaaa
     9421 aggtatcact tttgataaga cagaaatcaa gaagaattta accttgttct tgtatccaga
     9481 tgattcagat aaaaatggag aattattacg tatctatcag caatatttca tggtttcaaa
     9541 tgctgctcaa ttattaattg atgaagcaat cgagcgtggt tcaaaccttc atgatttagc
     9601 tgaatatgct tatgttcaaa ttaatgacac tcacccatct atggtcatcc ctgagttgat
     9661 tcgtttattg acagaaaaac atggttttga atttgacgaa gctgtgtcag ttgttcgcaa
     9721 tatggttggc tatacaaacc ataccatcct tgcagaagct cttgaaaaat ggcctttaga
     9781 gtatcttaat gaggtagtac cccacctagt aacaatcatt aaaaaattag atcaaatgat
     9841 tcgtgaagag caaacaaatc cagaggttca aattattgat gaagctggac gtgttcacat
     9901 ggcgcatatg gatatccact tctcaacaag cgttaatggt gtagcagcat tacatacaga
     9961 aatccttaaa aatagtgaat taaaggtttt ctatgatatt tacccagata agtttaacaa
    10021 taagacaaac ggtattacat tccgtcgctg gttggagttt gctaaccaag atttagcaga
    10081 ttacttgaaa gaactaattg gggatagcta tcttactgat gcgactcagc tcgaaaaatt
    10141 gttaacttat gctgatagca atgaagttca tgataaatta gctgctatta aatttaaaaa
    10201 caaactggct cttaaacgtt accttaagga aaataaaggt attgaattgg atgaatactc
    10261 aattattgat acacaaatca aacgattcca cgaatataag cgccaacaaa tgaatgcttt
    10321 atatgttatt cataaatact tagaaattaa acgtgggcat ttcccatcac gtaaattaac
    10381 agttattttt ggtggtaaag ctgctccagc ctatactatt gcacaggata tcattcactt
    10441 aattctctgc ttatcagaat taattaataa tgatccagaa gttaataaat accttaatgt
    10501 ccatttagta gaaaattaca atgtgacagt agcggaaaag ttaatccctg caacagatat
    10561 ttcggaacaa atttcattgg cttccaaaga agcatctggt acaggtaaca tgaaatttat
    10621 gttgaatggt gctcttactc ttggtactat ggatggggca aatgttgaaa tagctgaatt
    10681 agctggcaaa gagaacatct acacatttgg taaagactct gatactatca ttaatcttta
    10741 tgaaacatct ggttatcgtt caaaagatta ctatgataaa gacaaagtaa ttcgtgaagc
    10801 tgttgatttt atcattagtg atgatatagt gtcactcggt aatgcagaac gactaaagag
    10861 attgcatgat gagttagtag gtaaagattg gttcatgaca ttgattgatt tgaaagaata
    10921 cattgctgtt aaagaacaag tgcttgctga ttatgaagat tatgaatcat ggaataagaa
    10981 agttatacac aatattgcta aagcagggtt cttctcatca gatcgtacaa tcgaacaata
    11041 taatcaagat atttggcatt caaattaaaa agtctctaaa agtaaggttt tcaaccttac
    11101 ttttttcttg tctaataaaa ctataagtat ttatttttgt ccgtagactt taaaaataga
    11161 actgagcaga acttatt
//
LOCUS       DATRMA010000061        11134 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.061, whole genome shotgun sequence.
ACCESSION   DATRMA010000061 DATRMA010000000
VERSION     DATRMA010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11134
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.061"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            complement(36..833)
                     /locus_tag="VBC28_001529"
     CDS             complement(36..833)
                     /locus_tag="VBC28_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEO6815501.1"
                     /translation="MVRLIFSDIDGTLINSNFKVTPKTRQGIKQIVAQGATFVPISAR
                     MPEAITPIMEQIGIDSYIISYNGALIQDMQQKTIASHTMDGQVALQVCSYVSKHYSKI
                     AWNVYRYHEWYSCDKENEWVQKEEEIVGLQSKEMSLMELEKQDRIHKLLLMGEPSLMG
                     ELENTLKAQYPHLSIAQSAPYFIEIMAPGIEKGKSAKTLADYLDISLADSIAFGDNYN
                     DLNLLEIVGKGFVMGNAPKDLQERIGNVTQDNDNDGIYYALVEYMEN"
     gene            1019..1879
                     /locus_tag="VBC28_001530"
     CDS             1019..1879
                     /locus_tag="VBC28_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEO6815502.1"
                     /translation="MSYPYKANHSIESITLKVNDLENLVNFYSDIIGLTVIDKSSTRA
                     LLGVNQKIPLIILEKTELEKHSTYGLYHTAILVPDEYHLSLALNHLLSQHIPLEGGAD
                     HGYSNAIYLSDPEGNGIEIYNDKDISMWDIRESGQIIGITERLDIDNLLDSLVNVPNN
                     YKLSEKTSIGHIHLSVKDAKISSKLYQNVFGLDEKFAIPTASWIASGNYHHHLAFNNW
                     AGPNLSKNQEDRPGISLLTIAYNDDNLFRDSLKKAQLYQLTFLEKQDHYYIIEDFDGI
                     RIKVVLKPEN"
     gene            1922..2653
                     /gene="yaaA"
                     /locus_tag="VBC28_001531"
     CDS             1922..2653
                     /gene="yaaA"
                     /locus_tag="VBC28_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HEO6815503.1"
                     /translation="MIKILIPTAKEMKVCQNIAWPKLSAQTKIIIDYFSTLTVSDLED
                     IYRINTSAARCEAQRWQDFKAKQLTLNPAIKLFNGLMYRNIKRHNLSTSEAQFMENSV
                     FITSALYGIIPAMTLISPHRLDFNTKIKINNNSLKVFWRENYDTFMQSDDIMVSLLSN
                     EFETVFSPKERQKLIHLNFIEDRDGQLKTHSTISKKARGKCLTAMMENNCQTLEHLKQ
                     LRFDGFCYDNELSDSKQLTFVKKQT"
     gene            complement(3010..3627)
                     /gene="nrdG"
                     /locus_tag="VBC28_001532"
     CDS             complement(3010..3627)
                     /gene="nrdG"
                     /locus_tag="VBC28_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HEO6815504.1"
                     /translation="MSEEASWNTPKPGEWKSEELSHGHIIDYKAFNFVDGEGVRNSLY
                     VAGCMFHCKGCYNTATWSFRAGIPYTKELEDQIMTDLEQPYVQGLTLLGGEPFLNTGI
                     LLPLLQRIRRELPEKDIWSWTGYTWEEMMLETQDKLEMLSLIDILVDGRFDQSKRNLM
                     LQFRGSSNQRIIDVQKSLKEGEVVIWEGLNDGDNSYEQVKRDDLL"
     gene            complement(3700..4191)
                     /locus_tag="VBC28_001533"
     CDS             complement(3700..4191)
                     /locus_tag="VBC28_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO6815505.1"
                     /translation="MKLRRPVLEDKEEILAMYKEFQKESSSVDGGFYEPTMHFEDWLD
                     HNLNMELGVGVPDNFVPYIQFVSFDNDNNAIGFLNLRLRLNDTLLEKGGHIGYSIRPR
                     QRGKGYAKEQLKLGIEQAHLKNINEILVTCHVDNDASKSVILANGGVLEDCLHQTERY
                     WIT"
     gene            complement(4200..5132)
                     /locus_tag="VBC28_001534"
     CDS             complement(4200..5132)
                     /locus_tag="VBC28_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEO6815506.1"
                     /translation="MLQLGIVGLGGISQKAYLPYMRQVTGVHWHLFTRQKQILEEVNM
                     LFGSSTAYDSLDSLAEHPLDGVFIHVATSAHFDIAKLFLKKGIPVFMDKPLTEDYTST
                     KALYDLAKDHKTFLMAGFNRRFAPRIMEMKKVEDKNHIRTFKNAVNAPADFQYKLFDM
                     FIHPLDTALFLTNNVVKRGYFVTKRDGNKILQVSVTLETDSEIIEASMNLQSGSRREI
                     IEIESPEVTYSLDDLSNLSVIDGFDRRAIGFGSWASTLEKRGFEPMIDAFIQAITTGV
                     NPISPKSSLLSHFICDQINKANAPFGMLNLKIME"
     gene            complement(5145..5288)
                     /locus_tag="VBC28_001535"
     CDS             complement(5145..5288)
                     /locus_tag="VBC28_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6815507.1"
                     /translation="MGKYQLDYKGQAQVQKFHEKHSTGENANQKSRLKDLRKQFLEKA
                     KKK"
     gene            complement(5363..7561)
                     /gene="nrdD"
                     /locus_tag="VBC28_001536"
     CDS             complement(5363..7561)
                     /gene="nrdD"
                     /locus_tag="VBC28_001536"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HEO6815508.1"
                     /translation="MMVLERERFMTESDIKVIKRDGRLVSFDKYKIYTALLKASNKVI
                     KMSPLVEAKLEMIADHVIAEIYNRFKDNIKIYEIQNIVEHKLLEANEYAIAQEYINYR
                     TQRDFERSQATDINFSIGKLINKDQTVVNENANKDSDVFNTQRDLTAGIVGKSIGLKM
                     LPSHVANAHQKGDIHYHDLDYSPYTPMTNCCLIDFKGMLANGFKIGNAEVESPKSIQT
                     ATAQISQIIANVASSQYGGCTADRIDEFLAPYAQLNYQKHLKDAKEWVIEDKQEDYAR
                     AKTQKDIYDAMQSLEYEINTLFTSNGQTPFTSLGFGLGTNWFEREIQKAILKIRIQGL
                     GSEHRTAIFPKLIFTLKKGLNLEEDSPNYDIKQLALECATKRMYPDVLSYDKIIDLTG
                     SFKAPMGCRSFLQGWRDANGQDVTSGRMNLGVVTVNLPRVAMESNGDMDKFWEIFNER
                     MSIARDALVYRVERVKEAIPANAPILYQYGAFGERLGKYDNVDRLFNHRRATVSLGYI
                     GLYEVASVFYGGDWEDNHQAKAFTVDIVRKMKQLCADWSDEYDYHFSVYSTPSESLTD
                     RFCRLDTEKFGIVTDITDKEYYTNSFHYDVRKNPTPFEKLDFEKIYPETGASGGFIHY
                     CEYPVLQQNPKALEAVWDYAYDRVGYLGTNTPIDKCYQCQFEGDFTPTDRGFTCPNCG
                     NSDPKTVDVVKRTCGYLGNPQARPMVNGRHKEISARVKHMNGSSIKNQGN"
     gene            complement(7660..9201)
                     /locus_tag="VBC28_001537"
     CDS             complement(7660..9201)
                     /locus_tag="VBC28_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6815509.1"
                     /translation="MVENLEKPIGVSYKNSPSMSKRTAIRMKKSSRFSILLYSVLSTL
                     LAIANPLLTYFANGLQTQNLYTGLMMTKGQIPYSDVFATGGFLYYVTIALSYLLGSSI
                     WLLIVQFIAYYVSGIYFYKLVYYVAQSEIVSIGMTLIFYIMNIVLGFGGMYPIQWALP
                     FMLISLWFLIKFCVDNIVDEAFIFYGILAAFSLFIDPQTLIFWLCSFVLLTATNIKQK
                     QSLRGFYQFLCVVFGMILIAYTVGYFMFNLQIISSYIDKAIFYPFTYFARTNHSFLLS
                     LAIQIVVLLGSGCLFGLWDFIQNRKKASYQIGLNFIACIFIIYAIMAIFSRDFNLYHF
                     LPALPFGLLLTSNKITILYQKVIDRRSHRRQYFSGKSLIVDLFVKKTYYLPLLLVSLS
                     IGLLVYNTYQNVTLSKERRDISHYLTTKIDRDGKIYVWDKVASIYSQTRLKSASQFVL
                     PHINTAQKNNEKILKDELLQHGAKYFILNKNEKLPNELKSDIKKHYQEVPLSNITHFV
                     LYRFK"
     gene            complement(9451..9768)
                     /locus_tag="VBC28_001538"
     CDS             complement(9451..9768)
                     /locus_tag="VBC28_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HEO6815510.1"
                     /translation="MAHNHNHDHNHEHQHEVITLVDENGNETLFEILLTIDGREEFGK
                     NYVLLVPAGAEEDEQGEIEIQAYSFTENADGTEGDLQPIPEDSDAEWDMIEEVFNSFL
                     DEE"
     gene            complement(9794..10213)
                     /gene="ruvX"
                     /locus_tag="VBC28_001539"
     CDS             complement(9794..10213)
                     /gene="ruvX"
                     /locus_tag="VBC28_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003090938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEO6815511.1"
                     /translation="MRIMGLDVGSKTVGVAISDPLGFTAQGLEIIKIDEESGNFGFDR
                     LAELVKEYKVDKFVVGLPKNMNNTSGPRVEASQAYGDKITELFNLPVEYQDERLTTVQ
                     AERMLVEQADISRGKRKKVIDKLAAQLILQNYLDRMF"
     gene            complement(10213..10479)
                     /locus_tag="VBC28_001540"
     CDS             complement(10213..10479)
                     /locus_tag="VBC28_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HEO6815512.1"
                     /translation="MGFTDETVRFRLDDSNKVEISETLTAVYRSLEEKGYNPINQIVG
                     YVLSGDPAYVPRYNDARNQIRKYERDEIVEELVRYYLQGNGIDL"
     gene            complement(10681..11079)
                     /gene="spx"
                     /locus_tag="VBC28_001541"
     CDS             complement(10681..11079)
                     /gene="spx"
                     /locus_tag="VBC28_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HEO6815513.1"
                     /translation="MIKIYTISSCTSCKKAKTWLNAHQLPYKEQNLGKESLTRDEILE
                     ILTKTESGIESIVSSKNRYAKALNCNIEELSVNEVIDLIQENPRILKSPILIDDKRLQ
                     VGYKEDDIRAFLPRSIRNVENAEARLRAAL"
BASE COUNT         3643 a         2159 c         1646 g         3686 t
ORIGIN      
        1 taacacgctt ttaaggaaat ttagatttgc tatccttaat tttccatata ctctactaaa
       61 gcataataaa taccatcgtt atcattatcc tgagttacat ttccaattct ctcttgtaaa
      121 tctttaggag cattccccat gacaaaacct ttacccacaa tttcaagcag atttaaatca
      181 ttatagttat caccaaacgc aatagagtct gcaagagaaa tatctaaata gtctgctaaa
      241 gttttagcag attttccttt ttcaatacct ggagccatta tttcaataaa gtaaggagca
      301 gattgtgcga ttgataaatg tggatattga gctttcaaag tgttctccaa ttctcccatc
      361 agagatggtt ctcccatcaa gagaagttta tgaatcctat cttgcttctc taactccatc
      421 aaactcatct ctttagattg taaaccaaca atctcctcct ctttttggac ccactcattt
      481 tctttatcac aggaatacca ttcatgataa cgatagacat tccatgcaat tttcgaataa
      541 tgcttgctta cataagaaca aacttgtaag gcaacttgac catccattgt gtgggaagca
      601 attgtttttt gttgcatatc ttgtattaaa gccccattat aggaaataat ataagaatct
      661 attccaattt gttccataat cggagtaatt gcctcaggca tcctagctga tatgggaacg
      721 aaagtagccc cttgagctac tatttgttta attccctgcc gtgtcttagg tgttacttta
      781 aaattactgt taattaaagt accatcaata tcactaaata ttagccttac catattatcc
      841 tccaaaatat aattgtacac gcttacttta gtctaacact taaatttttg tttcgcaaat
      901 aaacttcgtc tggtctaatg agactactaa tataagctat atttcaaaac aaattagttt
      961 atttctctca atgaacggtg taatatgtta ctatatagta ataataggag gattaactat
     1021 gtcgtatcca tacaaagcca atcattccat tgaatcaatc accttaaaag taaatgacct
     1081 cgaaaacctc gtaaacttct atagcgatat cattgggtta actgttattg acaaatcaag
     1141 cactagagcc ttactagggg taaatcaaaa aatccctctt attatccttg aaaaaactga
     1201 acttgaaaag catagtacct atggccttta tcacactgct attttagtac cagatgaata
     1261 ccatctcagt ctagctctaa atcatttatt atctcagcat atccctctag agggtggagc
     1321 tgaccatggc tatagcaatg ctatctatct aagtgatcct gaaggtaatg gaattgaaat
     1381 ttataatgat aaggatatct ctatgtggga tatcagagag agtggacaaa ttattggtat
     1441 tactgaaaga ctagatattg acaatttact tgacagcctt gtcaacgttc ctaataatta
     1501 taagctttct gaaaaaacta gtattggtca tattcatttg agtgttaaag atgctaagat
     1561 tagctcaaaa ctctatcaaa atgtttttgg ccttgatgag aaatttgcaa tcccgacagc
     1621 tagttggatt gcatccggca actaccatca tcatctagcc tttaataatt gggctggtcc
     1681 aaatctttct aaaaaccaag aagatagacc cggaatttcc ttattgacaa ttgcttacaa
     1741 tgatgacaac ctttttagag atagcttaaa aaaagcgcaa ctttaccaac tgactttcct
     1801 tgaaaaacaa gatcattatt atattattga agattttgat ggcatccgaa ttaaagttgt
     1861 tctaaaacca gagaattaac catcacttga cttttgcctc ttcattttac ataatgtcaa
     1921 tatgataaaa attttgatcc caactgcaaa agaaatgaaa gtgtgtcaaa acattgcatg
     1981 gcctaaactg agtgctcaaa cgaaaattat aattgattat ttttctactt taactgtttc
     2041 agacttagag gatatctaca gaattaatac aagtgcagct cgttgtgagg ctcaaagatg
     2101 gcaagatttt aaagctaaac aacttactct gaatcctgct attaagttat tcaacggtct
     2161 tatgtaccgc aatatcaaaa ggcacaactt atccacaagt gaagcacaat ttatggaaaa
     2221 ttctgttttt attactagtg ctctttatgg catcattcct gctatgacac taatatcccc
     2281 tcatcgttta gattttaaca ctaaaatcaa aatcaacaat aactctctta aagttttttg
     2341 gcgtgaaaat tacgatactt ttatgcagtc agatgacatt atggtttctt tgctttctaa
     2401 tgaatttgaa actgtctttt ctcctaaaga gcgtcaaaaa ctaatacatc ttaactttat
     2461 tgaagatcga gatggccaat taaaaaccca ctctaccata tcaaaaaaag caagaggaaa
     2521 gtgtttaact gctatgatgg aaaataattg ccaaactcta gaacacttaa agcaattacg
     2581 ctttgatggc ttctgttatg ataatgaact ctcagactct aagcaattaa cttttgttaa
     2641 aaaacagact taagtaacta tttatccaca atttattcac acaaaaagca agagataatc
     2701 gtatctcttg ctttttgaca aacttgtaag gtctaaaaac ctctctatat caaggtttca
     2761 aacaatacta acaaaaaata attgttatta acaacttgtg gataactatg gggatagaca
     2821 gaggataatc tgtttatata aagttgatta cttaacacta atgtcattgt atataccttt
     2881 agatttcaaa aatacttgtt aaattaacaa taataaacgt caacatcttg tcacagactg
     2941 tcaaatactt ataaacaagt tgtggataaa cataactttt aatttttatt taaaagttaa
     3001 tttttgacac tagagtaaat catctcgttt gacttgttca taactattgt caccatcgtt
     3061 taatccttcc caaatcacga cttcaccttc ttttaaagat ttttgtacat caatgattct
     3121 ttgatttgaa gatcctctaa attgtagcat taaatttcgc ttactttgat caaacctacc
     3181 atccactaaa atatcaatca aagataacat ctccaattta tcttgagtct ccagcatcat
     3241 ttcttcccaa gtataccccg tccatgacca aatatctttt tctggcaatt cacgtcggat
     3301 acgctgaaga agtggcaaca aaatacctgt atttaagaac ggctctccac ctaataacgt
     3361 taaaccttgg acgtaaggtt gttctaaatc tgtcataatt tgatcttcaa gctcttttgt
     3421 ataagggatt ccggctcgaa aggaccatgt agcagtatta taacaccctt tacagtggaa
     3481 catacatcca gctacgtaga gtgagtttct aacaccttca ccatctacaa aattaaaagc
     3541 tttataatca atgatatggc catgacttaa ttcttcgctt ttccactccc caggctttgg
     3601 agtattccaa cttgcttctt ctgacatttc taacctctct attgatatga aaagaaccag
     3661 aactaactgg tctttttatt attatcttaa agtactattt taggttatcc aataacgctc
     3721 tgtctgatga agacaatctt ctaaaacacc accgttagct aatattactg acttgctggc
     3781 atcattgtct acatggcatg tcacaagtat ttcattaata ttttttagat gtgcttgctc
     3841 aattcctagc ttcaactgtt ctttagcata acctttgcca cgctggcgag gtcgtatgga
     3901 ataaccaata tgtccacctt tttctaaaag agtatcattt agtcttaaac gtaaatttaa
     3961 gaaacctata gcattattgt cattgtcaaa agacacaaac tgaatatatg gaacaaaatt
     4021 atcaggaacg cctaccccta actccatatt taggttatga tctagccagt cttcaaaatg
     4081 catagttggt tcataaaaac caccgtccac tgaactactt tctttttgaa actctttata
     4141 catggctaaa atttcttctt tatcttctaa cactgggcgc cgtaatttca tattgtttcc
     4201 tattccatta tcttcaagtt aagcattcca aaaggagcat tcgctttatt aatctggtca
     4261 caaataaaat gactcaataa actagacttt ggtgatatag gattgacacc tgtagtgata
     4321 gcttgaataa aagcatcaat cattggttca aaaccacgtt tctctagagt gcttgcccaa
     4381 gaaccaaaac cgatagcacg acggtcaaag ccatcaataa cagaaagatt tgacaaatca
     4441 tctaatgaat aggttacttc tggcgactct atttcaatta tttctcgacg agaccccgac
     4501 tgcaggttca tgcttgcttc tattatctca ctgtctgttt ctagcgttac tgatacttgt
     4561 aatatcttat tcccatcacg tttagtcaca aagtatcctc ttttaacaac attatttgtc
     4621 aaaaatagcg ccgtatctaa aggatggata aacatatcaa aaagcttata ttgaaaatcc
     4681 gctggtgcat tgacagcatt tttaaaggtg cgaatatggt ttttatcttc tacttttttc
     4741 atttccataa tacgtggtgc aaagcgacga ttaaaacctg ccattaaaaa tgttttatga
     4801 tccttcgcca aatcatacaa tgctttagtt gaagtataat cttctgtcaa aggcttatcc
     4861 ataaaaactg gaataccctt ttttaaaaaa agctttgcta tatcaaagtg agcactagtc
     4921 gcgacatgaa tgaagactcc atccaaagga tgctcggcta gactatctaa actgtcataa
     4981 gctgtagaag aaccaaaaag catattaact tcttctaaaa tctgtttttg gcgtgtaaaa
     5041 agatgccagt gcacacctgt cacttgacgc atataaggta gataagcttt ctgtgaaata
     5101 cctcctaaac cgacaattcc aagttgcaac atagcatcct ccttctattt tttctttgcc
     5161 ttttccaaaa attgtttcct taaatctttt agacgtgatt tttgattagc attctctcca
     5221 gtagagtgct tctcatggaa cttctgtact tgtgcttgcc ctttgtaatc taattggtat
     5281 ttccccatat cttaataact ccttctattt tatcttctta ttttaacata taatagacca
     5341 gaggttttaa tagaggtttt aattaattac cttgattttt aatacttgat ccgttcatat
     5401 gttttacacg agcagagatt tccttatgac gaccattaac catcggacga gcctgtggat
     5461 tacctaaata accacatgta cgtttgacaa catctactgt cttagggtcg ctgttaccac
     5521 aatttggaca agtaaaacct cgatctgtcg gtgtaaaatc cccttcaaat tgacattggt
     5581 aacacttatc aatcggtgta ttagtaccta gataacctac ccgatcataa gcatagtccc
     5641 aaactgcttc taaagctttt ggattctgtt gcaaaacagg gtattcacag tagtgaataa
     5701 atcctcctga tgcacccgtt tcaggataga ttttctcaaa atctagtttt tcaaaaggtg
     5761 ttggattctt acgcacatca taatggaatg aattagtgta gtattcctta tcagtaatat
     5821 cagttactat accaaatttt tcagtatcta aacgacaaaa ccggtcagtc aagctctccg
     5881 atggtgtcga ataaactgaa aaatgataat catactcatc agaccaatct gcacataact
     5941 gcttcatctt acgaacgatg tctacagtaa atgctttagc ttgatggtta tcttcccagt
     6001 caccaccata aaaaacactc gccacctcat ataaaccaat ataaccaaga gacaccgttg
     6061 cacggcggtg attaaagagt cgatcaacat tgtcatattt tcctaaacgt tctccaaatg
     6121 ctccatactg gtaaagaatg ggcgcatttg ctgggatagc ctctttgaca cgttccacac
     6181 gataaaccaa ggcatctcta gcaatactca tacgttcatt aaatatctcc cagaatttat
     6241 ccatatcacc gttagactcc atggcaacac gcggtagatt aactgttaca actccaagat
     6301 tcatacgacc agatgttaca tcctgaccat ttgcatctcg ccacccttgt aggaaagaac
     6361 gacagcccat aggggcttta aaagagcccg ttaagtcaat aattttatca taagaaagga
     6421 catctggata catccgtttt gtcgcacact ctaatgctaa ttgtttaata tcataattgg
     6481 ggctatcttc ttctaaatta agaccttttt taagcgtaaa aattaatttt ggaaaaatag
     6541 cagtacgatg ctcgcttcct aaaccttgaa tccgaatttt caaaatcgct ttttgaattt
     6601 ctcgttcaaa ccaattagtt cctaatccaa aaccaagcga agtaaatggc gtttgcccat
     6661 ttgaagtaaa taaagtatta atttcatact ccaaagattg catggcatca tatatatctt
     6721 tttgtgtttt agcgcgcgca tagtcttctt gtttatcttc aataacccat tcttttgcat
     6781 cttttaaatg tttttgataa tttaactgtg cataaggagc taaaaactca tcaatgcgat
     6841 ctgctgtaca gccaccatat tgacttgaag caacgttcgc aataatctgt gaaatctgag
     6901 cagtcgctgt ctgaatagat tttggactct caacttctgc attaccaatc ttaaaaccat
     6961 ttgcaagcat tcccttaaaa tcgattaaac agcaatttgt catgggagtg tatgggctgt
     7021 aatctaaatc atgataatgg atatcaccct tttgatgtgc atttgctaca tgagaaggta
     7081 acattttcag accaattgat ttccctacaa tacctgctgt taaatcacgt tgggtattaa
     7141 acacatcaga gtctttatta gcattctcat taacaacagt ttgatcctta ttaattaatt
     7201 ttccaataga aaaattgata tctgtagcct gtgaacgctc aaaatcgcgt tgggtacgat
     7261 aattgatata ttcttgtgca atagcgtact cattagcttc caataattta tgttcaacga
     7321 tattttgtat ttcatatatc ttgatgttat ctttaaaacg attgtagatc tcagcgatga
     7381 catgatctgc aatcatctca agtttagcct caactaaagg agacattttg ataactttat
     7441 tactagcttt taatagagcc gtgtagattt tatacttatc aaagctgacc aaacgcccgt
     7501 cacgtttaat cactttgata tcagattctg tcataaatcg ttctctttcc aataccatca
     7561 tgtgaaatat cctccagtga caattatagc atgcaaagaa aaaaaatcaa tatattgtgt
     7621 caagtttttt gctcagacac aatatattga ttttttaatc tatttgaaac gatatagaac
     7681 aaaatgagtg atatttgata gaggaacttc ttggtagtgc ttcttaatat cagatttcag
     7741 ttcatttggt aacttctcat ttttatttaa gataaagtat ttagccccat gctgaagcag
     7801 ctcgtccttc aaaattttct cattattttt ttgcgctgta ttaatatgtg gcaaaacaaa
     7861 ttgagaagca gattttaacc gagtctggct ataaatgcta gcaactttgt cccaaacata
     7921 aatcttacca tctctatcaa tttttgtagt caaataatgt gaaatgtcac gcctttcttt
     7981 actaagagta acattttgat aagtattata aactagtaac cctatcgata aggaaactag
     8041 taagaggggc aaataatatg ttttctttac aaaaaggtct acgataagag atttaccaga
     8101 gaaatactga cgtctatgtg aacgtctgtc aatcactttt tggtaaagaa tagtaatttt
     8161 attactcgtt agcaaaagac caaatggtaa agcaggtaaa aagtggtaaa ggttaaaatc
     8221 tcttgaaaaa atggccatta tagcgtaaat aataaaaata caggcaataa agttcaagcc
     8281 gatttgataa gatgcttttt tacgattttg aataaaatcc catagtccaa agagacaacc
     8341 tgaccctaga aggacaacaa tttgaattgc taaactaaga aggaatgagt ggtttgtcct
     8401 agcgaaatac gtaaaggggt aaaaaatagc tttgtcaata taagaagaga taatctgtag
     8461 attaaacata aagtaaccaa cagtataagc aattaaaatc ataccaaaaa caacgcataa
     8521 aaactgataa aatcctcgta gtgactgctt ttgcttaata ttggttgctg ttaagagaac
     8581 aaaagagcaa agccaaaaaa tcagtgtttg gggatctata aatagtgaga atgctgctaa
     8641 aatgccataa aatataaatg cttcatcaac gatattatcg acacaaaatt taattaaaaa
     8701 ccatagcgaa atgagcataa aaggtaatgc ccactgtatt gggtacatac caccgaatcc
     8761 taagacaata ttcattatat agaaaatcaa cgtcatgcct atcgagacaa tttcactttg
     8821 tgccacataa taaactaatt tataaaaata aattccagat acatagtaag caataaactg
     8881 tacaataagt aaccagatac tagaccctaa aaggtaactt agagcaatcg ttacatagta
     8941 taaaaatcct cctgtagcaa aaacatcact ataagggatc tgtcctttgg tcatcatcaa
     9001 acctgtatag agattctgag tctgcaaacc atttgcaaaa taagtcaata aaggatttgc
     9061 aattgctaag agagttgaca aaacactata tagtaaaatt gaaaatcgac tagatttctt
     9121 cattctgata gccgttcgtt tactcattga tgggctattt ttatagctca ctccaattgg
     9181 tttttctagg ttttctacca ctatttttct cctcatattt acatactacc agtatatcag
     9241 aaaaccttat ttagtcaatt tagccaacac ttgaccgtaa cgaataatat ccctaaagta
     9301 gtggtatttt ctagacatat attgtttgag aaattcttgt tcttctagtg ttagctgagt
     9361 atctgacatt aacatcacct ccttttacta catcatcact attattattc gaaaaaaaaa
     9421 cgactgaaca attgctcaat cgcttcgata ttattcttca tctagaaagc tgttgaaaac
     9481 ttcctcaatc atgtcccatt ctgcatctga atcttctggt ataggctgaa ggtctccttc
     9541 agttccatcg gcattttctg taaatgagta agcttgaatc tcaatttcac cttgttcgtc
     9601 ttcttccgca cctgcaggaa cgaggagaac atagtttttg ccaaactctt cacgcccatc
     9661 aatagttaat agaatttcaa aaagtgtttc gttcccattc tcatctacaa gagtgattac
     9721 ttcatgttga tgttcatggt tatgatcgtg gttatgatta tgtgccattg tttctccttt
     9781 ttatgccgaa tgcttaaaac atgcggtcta aataattttg taagattagt tgagctgcta
     9841 atttatcgat aactttctta cgcttaccac gactaatatc agcttgctca actagcatac
     9901 gctctgcttg aacagtcgtt aaacgttcat cttgatattc aactggaaga ttgaataatt
     9961 cagtgatttt atcgccatat gcctgactag cctctacacg aggaccactt gtattattca
    10021 tatttttagg tagaccaact acaaatttat caactttgta ctcttttact aactctgcta
    10081 agcggtcaaa accaaaattt ccagattctt catcgatctt aatgatttca agaccttgtg
    10141 cagtgaaacc gagtggatca ctaattgcaa cgccgacagt tttcgagccg acgtcaagtc
    10201 ccattattct cattacaaat ctattccatt tccttgtaaa taataacgaa cgagctcttc
    10261 tacaatctca tcacgttcat atttacgaat ttgattgcga gcatcattgt aacgaggtac
    10321 ataagcaggg tccccactca atacgtaacc aacaatttga ttgatgggat tgtaaccttt
    10381 ttcttctaat gaacgataaa cagctgttaa tgtttcacta atttctactt tgttactatc
    10441 atctaatcta aaacgaacag tttcatctgt aaatcccata cctacacctt ctttccttta
    10501 attctttcac ttattataac ttaaataaag tatttgtaca agaaattgat aaacactata
    10561 ttagaaaatc aaaaagggag cacaagaaac caagaagtga ttctttgatt tctttactcc
    10621 ctaccttata ggtgattttt acaggttatc gtgtgactct cacttatgct tatctaaagc
    10681 tcaaagtgct gcacgaagac gagcttctgc attttcaacg ttacgaatag agcgaggtag
    10741 aaaagcacga atatcatctt ccttataacc tacttgaaga cgtttgtcat caattagaat
    10801 tggactcttt aaaatacgtg gattttcttg aataagatca ataacttcat ttacactcaa
    10861 ctcctcaata ttacaattga gcgccttagc ataacgattt ttagaagaaa cgatactttc
    10921 aataccactt tcagtttttg ttaatatttc taaaatttca tcacgtgtta gtgattcttt
    10981 acctaggttt tgttctttat atggaagttg atgagcattt agccaagttt ttgctttttt
    11041 acaacttgtg caacttgaaa tagtataaat cttaatcatg tgccttctca ctttctatcc
    11101 catgatagtt atagtttaac atagtcacaa aaat
//
LOCUS       DATRMA010000062        11074 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.062, whole genome shotgun sequence.
ACCESSION   DATRMA010000062 DATRMA010000000
VERSION     DATRMA010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11074
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.062"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            complement(<1..323)
                     /locus_tag="VBC28_001542"
     CDS             complement(<1..323)
                     /locus_tag="VBC28_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000977218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3169 family protein"
                     /protein_id="HEO6815514.1"
                     /translation="MMKNFIKQSDTTFVRIIKSLLIGGFIGAILGSVGALFIIFGQDK
                     YLSEINIVQYFLWVSRIVVIITALFSLIYLYQIQKYQKVFFNVDESQSEEIYRQINLR
                     HSYGMT"
     gene            complement(323..538)
                     /locus_tag="VBC28_001543"
     CDS             complement(323..538)
                     /locus_tag="VBC28_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO6815515.1"
                     /translation="MGLILKNRLKELRARDGINQTELAKLTGVSRQTISLIERGEYTP
                     SVIIAMKISQVFKESLENVFSLVSMEE"
     gene            complement(583..876)
                     /locus_tag="VBC28_001544"
     CDS             complement(583..876)
                     /locus_tag="VBC28_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6815516.1"
                     /translation="MVMTYIGVPKEQKALSRRGKQLEKINQGGKTLMSQMIQDLVGST
                     CMIYLEDGSQTHPYEILKVDEDWVQIRRNLKRSKEEVRIVRIHDIKELRPVNK"
     gene            complement(909..1097)
                     /locus_tag="VBC28_001545"
     CDS             complement(909..1097)
                     /locus_tag="VBC28_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6815517.1"
                     /translation="MKKEFIITGRMVARVMETLVCLCMVSNLVLDMLDIIEINEIVFC
                     VLVASIAGLEFCLQKERE"
     gene            complement(1298..2116)
                     /locus_tag="VBC28_001546"
     CDS             complement(1298..2116)
                     /locus_tag="VBC28_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HEO6815518.1"
                     /translation="MNLLFSIDDMYVDHFKVMLYSLVRQTKNRKLEIYVLQKTLLKRH
                     TELIQYTQNLEVGYHPIIVGTEVFAQAPTTDRYPDTIYYRLLAHKFLPETLDRILYLD
                     ADMLCLNDFSSLYDMELGDQLYAAASHNTDGKFLDYVNKLRLKNVELESSYFNTGVLL
                     MNLPAIRKVVHQQTILDYIMQNRGRLILPDQDILNGLYANLVKPIPDEIYNYDARYSL
                     IYQLKSRNEWDLEWVINHTVFLHFAGRDKPWKKDYRGRYSGLYKFMAKEALTKN"
     gene            complement(2204..2920)
                     /locus_tag="VBC28_001547"
     CDS             complement(2204..2920)
                     /locus_tag="VBC28_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000232920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HEO6815519.1"
                     /translation="MVGLGTVINVILIIVGGFVGLFLKNFLKESLQKSLMQAMGVAVL
                     FISISGVLEKMMLVEKSHLISNHTNMMIITLALGTVLGELLSLDSYIDKFGNYLKQKT
                     GSGNDIKFVEAFVTSTCTVCIGAMAVVGSIQDGIAADHSILFAKGMLDMIIIAIMTVS
                     LGKGALFSALPVALLQGSLTIVAFFMGSLLNPSSLDYLNLVGNMLIFCVGVNLLFNLN
                     IKVINMLPAIILAILWGSFI"
     gene            complement(3002..5620)
                     /gene="alaS"
                     /locus_tag="VBC28_001548"
     CDS             complement(3002..5620)
                     /gene="alaS"
                     /locus_tag="VBC28_001548"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEO6815520.1"
                     /translation="MKELSSAQIRQMWLDFWKSKGHSVEPSANLVPVNDPTLLWINSG
                     VATLKKYFDGSVIPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFRD
                     EAIEWGFELLTSPEWFDFPKDKLYMTYYPDDKDSYNRWIACGVEPSHLVPIEDNFWEI
                     GAGPSGPDTEIFFDRGEDFDPENIGLRLLAEDIENDRYIEIWNIVLSQFNADPAVPRS
                     EYKELPNKNIDTGAGLERLAAVMQGAKTNFETDLFMPIIREVEKLSGKTYDPDGDNMS
                     FKVIADHIRALSFAIGDGALPGNEGRGYVLRRLLRRAVMHGRRLGINETFLYKLVPTV
                     GQIMESYYPEVLEKRDFIEKIVKREEETFARTIDAGSGHLDSLLAQLKAEGKDTLEGK
                     DIFKLYDTYGFPVELTEELAEDAGYKIDHEGFKSAMKEQQDRARAAVVKGGSMGMQNE
                     TLAGIVEESRFEYDTYSLESSLSVIIADNERTEAVSEGQALLVFAQTPFYAEMGGQVA
                     DHGVIKNDKGDTVAEVVDVQKAPNGQPLHTVNVLASLSVGTNYTLEINKERRLAVEKN
                     HTATHLLHAALHNVIGEHATQAGSLNEEEFLRFDFTHFEAVSNEELRHIEQEVNEQIW
                     NDLTITTTETDVETAKEMGAMALFGEKYGKVVRVVQIGNYSVELCGGTHLNNSSEIGL
                     FKIVKEEGIGSGTRRIIAVTGRQAFEAYRNQEDALKEIAATVKAPQLKDAAAKVQALS
                     DSLRDLQKENVELKEKAAAAAAGDVFKDIQEAKGVRFIASQVDVADAGALRTFADNWK
                     QKDYSDVLVLVAAIGEKVNVLVASKTKDVHAGNMIKGLAPIVAGRGGGKPDMAMAGGS
                     DASKIAELLAAVAENL"
     gene            complement(5636..6121)
                     /locus_tag="VBC28_001549"
     CDS             complement(5636..6121)
                     /locus_tag="VBC28_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related family protein"
                     /protein_id="HEO6815521.1"
                     /translation="MATFQIKEKMFSLGGKFTITDLTGLPCYHVEGSLFPLPKTFKVF
                     DEEEHLISQIEKKVLSFLPKFNVTLANGNHFTIKKDFSFLKPHYTIEDLDMEVKGNFW
                     DMDFQLLKDNQVIANISQQWFRMTSTYQVEVYSETYNDLTISLVIAIDYVKELEKNAS
                     N"
     gene            complement(6363..7292)
                     /gene="prsA"
                     /locus_tag="VBC28_001550"
     CDS             complement(6363..7292)
                     /gene="prsA"
                     /locus_tag="VBC28_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PrsA"
                     /protein_id="HEO6815522.1"
                     /translation="MKTRSKLAAGFLTLMSVATLAACSGKTSNGTNVVTMKGDTITVS
                     DFYDQVKTSKAAQQSMLTLILSRVFDTQYGDKVSDKKVSEAYNKTAKGYGNSFSSALS
                     QAGLTPEGYKQQIRTTMLVEYAVKEAAKKELTEANYKEAYKNYTPETSVQVIKLDAED
                     KAKSVLKDVKADGADFAKIAKEKTTATDKKVEYKFDSAGTTLPKEVMSAAFKLDKNGV
                     SDVVSTVDSTTYKTSYYIIKVTDKTEKKSDWKSYKNRLKEVILKDKTSDRAFQNKVIS
                     KALEKANVKIKDKAFAGILSQYATTSGSSSLKK"
     gene            complement(7353..8060)
                     /locus_tag="VBC28_001551"
     CDS             complement(7353..8060)
                     /locus_tag="VBC28_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEO6815523.1"
                     /translation="MVQSYSKNANHNMRRPVVKEEIVQYMRQHQKQNNGCLAELEAFA
                     KQENIPIIPHETATYFRFLMQTLQPKHILEIGTAIGFSALLMAENAPEAKITTIDRNE
                     EMIALAKENFAKYDNHNQITLLEGDAVDVLQTLDKSYDFVFMDSAKSKYIVFLPQVLK
                     HLDVGGVVVLDDIFQGGDIAKPIDEVRRGQRTIYRGLQRLFDSTLQHPDLTATLVPLG
                     DGLLMIRKNADHIVLED"
     gene            complement(8134..8760)
                     /locus_tag="VBC28_001552"
     CDS             complement(8134..8760)
                     /locus_tag="VBC28_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HEO6815524.1"
                     /translation="MKKLTFIWDLDGTLIDSYVPIMEALEETYRHFGLIFDKELIHEY
                     ILQESVGKLLVNLSEEEQIPHEKLKAYFTKEQESRDSKIHLMPYAKEILEWTKEQDIP
                     NFMYTHKGASTHSVLETLQISHYFDEILTGVSGFERKPHPQGINYLVKRYSLDKSMTY
                     YIGDRPLDLEVAQNAGIKSINLRLENSKENYNISSLKDIISLDFTRLD"
     gene            complement(8955..10760)
                     /gene="pepF"
                     /locus_tag="VBC28_001553"
     CDS             complement(8955..10760)
                     /gene="pepF"
                     /locus_tag="VBC28_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEO6815525.1"
                     /translation="MSDNRSHIEEKYQWDLTTVFATDELWETEVVELTQAIDNAKGFS
                     GHLLDSSQSLLEITEVELDLSRRLEKVYVYASMKNDQDTTVAKYQEFQAKATALYAKF
                     SETFSFYEPELLQLSESDYQSFLLEMPDLQKYDHFFEKIFANKPHVLSQNEEELLAGA
                     SEIFGAAGETFEILDNADMVFPVVKNAKGEEVELTHGNFISLMESSDRTVRKEAYQAM
                     YSTYEQFQHTYAKTLQTNVKSQNFKARVHHYQSARQSALSANFIPEEVYETLIKTVNH
                     HLPLLHRYMKLRQKVLGLDDLKMYDVYTPLSQMDMSFTYDEALKKSEEVLAIFGEAYS
                     ERVHRAFTERWIDVHVNKGKRSGAYSGGSYDTNAFMLLNWQDTLDNLYTLVHETGHSL
                     HSTFTRENQPYVYGDYSIFLAEIASTTNENILTETLLKEVKDDKNRFAILNHYLDGFK
                     GTIFRQTQFAEFEHAIHVADQEGQVLTSEYLNNLYAELNEKYYGLTKEDNHFIQYEWA
                     RIPHFYYNYYVFQYATGFAAANYLAERIVNGNPEDKEAYLNYLKAGNSDYPLNVIAKA
                     GVDMTSADYLDAAFRVFEERLVELENLVAKGVHND"
     gene            complement(10776..>11074)
                     /locus_tag="VBC28_001554"
     CDS             complement(10776..>11074)
                     /locus_tag="VBC28_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000903651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HEO6815526.1"
                     /translation="KQDRNIRDYVRQQLRYGNQFWLRKQEKAYLSGQNLLTQELMMFF
                     PQIQPPRVDTDFCQITNSLTSFYQNFTNYYQKNKNNLDQTLYPPVFYDKIVTKN"
BASE COUNT         3402 a         2174 c         1764 g         3734 t
ORIGIN      
        1 gtcatcccat aagaatgtct taaattaatc tggcgataaa tttcttcaga ttgactttca
       61 tcaacattga aaaaaacttt ctgatatttt tgaatctgat agaggtagat gagactaaag
      121 agagctgtta taattacaac aatacgacta acccatagga aatattgaac aatattaatt
      181 tcagataagt acttatcttg accaaatatg ataaaaagag ctcctacgga accaagtatg
      241 gcccctataa aaccaccaat taaaagactt ttgataattc gaacaaatgt agtatcactt
      301 tgttttataa aatttttcat cattattcct ccattgacac taaactaaaa acattctcta
      361 gggattcttt aaatacttga gaaatcttca tagcaataat aacagatggc gtatattctc
      421 cgcgttcaat taggctaatt gtttgtcttg agactcctgt taatttagcg agttctgttt
      481 gattgatacc atctcgtgca cgcaattctt tgagtctatt ttttaaaatt aatcccatca
      541 tttttctcct ttataaaatg atatatagat ttattcctcg ctctatttat tgactggacg
      601 caattccttg atatcatgaa tacggacaat acgcacctcc tctttacttc gtttcaagtt
      661 gcgacgaatc tgtacccaat cctcatctac ctttaaaatc tcataaggat gagtttgaga
      721 accatcctct aaataaatca tacatgtgct tcctactaaa tcttgaatca tttgtgacat
      781 aagtgttttt cctccctgat ttattttttc taattgttta cccctacgac tgagtgcctt
      841 ttgctcttta ggtacaccaa tatacgtcat gaccataaaa agccattaag ccaaacaatt
      901 ccatctatct actccctttc tttttgcaaa caaaactcaa gccctgcaat tgatgctacc
      961 aacacacaaa atacaatttc atttatttct attatatcaa gcatatcaag aacaagatta
     1021 cttaccatac ataagcaaac tagggtttcc ataactcttg caaccatccg acctgtaata
     1081 ataaattcct ttttcatttg agtaactcct aaaaccatta tgcttttgaa acaatgatta
     1141 atttgattgt tcaatgatta agtgatttta acacgctcct cctttgatat tatttttctt
     1201 ttttataaat aattattagc aaagattatt gatatcatta aaatatcacc ccaaaaaaag
     1261 ttactaaata gaaataattt agtaaccttt taaatcttca atttttagtt aaagcctctt
     1321 tagccataaa cttataaagg cccgagtaac gtcctctata atcttttttc catggtttat
     1381 ctcgtcctgc aaaatgtaaa aaaacagtat ggttaataac ccactctaaa tcccattcat
     1441 tgcgactttt taattgataa ataagactat acctagcatc ataattataa atttcatcag
     1501 gtattggttt aaccaaatta gcgtataagc catttaagat atcctgatct ggtaagatta
     1561 gacgaccacg gttttgcatt atgtaatcta agatagtttg ttgatgaaca acttttcgta
     1621 tagcaggcaa gttcatcaag agtacccctg tattgaaata agaggattct agctctacat
     1681 tttttaaccg taatttattg acataatcta gaaacttacc atcagtatta tgacttgccg
     1741 ctgcatatag ttgatcacct aactccatat catataaact tgagaaatca tttaagcaaa
     1801 gcatatctgc atctaaataa agtatcctat ctaaggtctc aggtaaaaat ttatgagcca
     1861 aaagtcgata atatattgta tctggatagc gatcagttgt aggtgcctga gcaaatactt
     1921 cggtacctac aattattgga tgatagccaa cttctaaatt ttgagtatat tgaataagtt
     1981 ctgtatgacg tttgagtaat gttttttgta agacatatat ctctaactta cgattcttgg
     2041 tttgtcggac aagcgaataa agcatcactt taaaatgatc tacgtacata tcatcaatag
     2101 aaaataatag attcatgagt tggatcctct ccgtagtata tcttctttca tcctaacata
     2161 tgtctaaaga aaaaccaagt ttgagttaaa acttggttat cttttatata aaacttcccc
     2221 ataatatagc taaaataatt gctggcaaca tattgataac tttaatattt aggttaaaga
     2281 gtaaattaac accgacacag aatataagca tatttcctac taagttaagg taatctaaag
     2341 atgatggatt aagtagactc cccatgaaaa aagcaacaat cgtaaggcta ccttgtagta
     2401 aagcgacagg aagtgcagaa aatagagctc ctttccctaa agacactgtc ataattgcaa
     2461 taataatcat gtctaacatt cccttagcaa ataaaatcga atgatctgct gctataccat
     2521 cttgaatgct accaacaacg gccattgctc caatacaaac ggtacaagtt gaggtgacga
     2581 atgcctccac aaatttaata tcgttaccac ttcctgtttt ttgcttaaga tagttcccaa
     2641 atttatcaat atacgaatct agtgatagca actctccaag taccgtacct agagcaagag
     2701 taataatcat catatttgta tgattagaga tgagatgtga cttttcgact aacatcattt
     2761 tctccaaaac acctgatatc gaaataaata aaactgcaac tcccatggct tgcataaggc
     2821 tcttttgaag tgattctttc aaaaaatttt tcaaaaataa acctacaaag cctccaacta
     2881 taataagaat aacattgata actgttccta aaccaaccat atttctccta tatacacaaa
     2941 ggaacagaac gatgttctgc cctttgtctg ttttatttac taactaaatg agaggtgttt
     3001 cttataaatt ttctgcaact gctgctagca actctgcaat tttacttgca tcgctaccac
     3061 ctgccatagc catgtctggt ttaccaccac cacgacctgc tacaattggt gccaatcctt
     3121 tgatcatgtt accagcgtgg acatctttag ttttgcttgc aacaaggaca ttaaccttct
     3181 caccaatagc tgctacgaga acaagcacat cagagtagtc tttttgtttc cagttatcag
     3241 caaatgtacg aagtgcacct gcgtctgcaa catcaacttg actagcaatg aagcgcacgc
     3301 ccttagcttc ttgaatatct ttaaagacat caccagctgc tgcagctgct gctttttctt
     3361 taagctctac attttctttt tgaagatcac gaagtgagtc gctaagagct tgtactttag
     3421 ctgctgcatc tttcaattgc ggagctttta cagtagcagc gatctctttt agtgcatcct
     3481 cttggttacg ataagcttca aaagcttgtc taccagtaac tgcaataata cgacgagtac
     3541 ctgaaccaat accttcttct ttgacaatct tgaagagacc gatttctgaa gaattattta
     3601 agtgagttcc accgcaaagt tcaacagaat aattaccaat ttgaaccaca cgaaccactt
     3661 taccatattt ctcaccaaaa agtgccattg ctcccatctc ttttgcggtt tcaacgtcag
     3721 tttcagtcgt tgtgatcgta agatcgttcc aaatttgctc attaacttct tgttcaatgt
     3781 gacgaagttc ctcattgctt actgcttcaa agtgagtaaa atcaaagcgc aagaattctt
     3841 cttcgttcaa tgaaccagcc tgagttgcgt gttcaccgat aacattgtga agagctgcat
     3901 ggagcaagtg agttgctgtg tggtttttct caacagccaa acgacgctct ttgttgattt
     3961 caagtgtgta gtttgttcca actgaaagtg atgccaaaac gtttacagtg tgtagaggtt
     4021 gaccatttgg tgctttttga acatcaacaa cctcagcaac tgtgtcaccc ttatcatttt
     4081 tgatgacacc gtggtcagca acctgtccac ccatttcagc atagaatggt gtttgagcaa
     4141 agacaagaag agcttgacct tctgaaactg cttcagtacg ttcattgtca gcgatgatga
     4201 ctgaaagact tgattcaaga ctatatgtgt cgtattcgaa tcgtgattct tcaacaatac
     4261 cagctagggt ttcattttgc atccccatcg aaccaccctt aacaacagct gcacgcgcac
     4321 ggtcttgttg ttctttcatg gctgacttaa agccttcgtg gtcaatctta tagcctgcat
     4381 cttctgccaa ttcctctgtc aattcaactg ggaatccata agtatcataa agtttgaaga
     4441 tatctttacc ttcaagtgtg tctttacctt cagccttaag ctgcgcaagc aatgaatcta
     4501 agtgaccgct accagcatcg atagtacgag caaatgtttc ttcctcacgt ttaacgattt
     4561 tctcaataaa atcacgtttt tcaagcactt ctggatagta gctttccatg atttgtccaa
     4621 cagtcggaac caatttgtaa aggaaagttt cgttgatgcc aagacggcga ccgtgcataa
     4681 ccgcacgacg gagaagacga cgaagaacat aaccacgacc ttcatttcca ggaagcgcac
     4741 catcaccgat ggcaaatgaa agcgcacgga tgtgatcagc gataaccttg aagctcatgt
     4801 tgtcgccatc tggatcgtaa gttttacctg acaatttctc tacttcacgg atgataggca
     4861 tgaaaaggtc agtttcaaag tttgtttttg ccccttgcat aacagctgca agacgttcaa
     4921 gacccgcacc cgtatcaatg tttttgtttg gcaattcttt gtattctgaa cgtggtacgg
     4981 ctgggtcagc attgaattgt gagagaacaa tgttccagat ttcgatgtaa cgatcgtttt
     5041 cgatatcttc agccaaaaga cgaagtccaa tattttctgg atcaaaatct tcaccacggt
     5101 cgaagaaaat ctcagtatct ggacctgaag gaccagcacc gatttcccag aagttatcct
     5161 cgattggcac caagtgactt ggttcaacgc cacaagcaat ccaacggtta tatgaatcct
     5221 tgtcatctgg gtaataagtc atgtagagct tgtctttagg gaagtcaaac cattcaggac
     5281 ttgtcaagag ttcaaatccc cactcaatag cttcatcacg gaaatagtct ccaattgaga
     5341 agttaccaag catttcaaac atagtatggt gacgtgctgt tttaccaaca ttttcaatat
     5401 cattagtacg aattgatttt tgtgcattgg taatacgtgg attttctgga atcactgaac
     5461 catcaaaata ttttttcaag gttgcaacac ctgagttgat ccaaagaagc gttgggtcat
     5521 taacaggaac caagttagct gaaggctcaa cggaatgtcc tttggatttc cagaaatcca
     5581 accacatttg gcggatttgt gcagacgata attctttcat tattctctcc taaaattagt
     5641 ttgatgcatt tttttctaac tctttgacat agtcgatcgc tatgacaagc gaaattgtca
     5701 aatcgttgta agtttcactg taaacttcta cctgataagt tgaagtcata cgaaaccact
     5761 gttgagatat gtttgctata acttgattgt cttttaagag ttggaaatcc atatcccaaa
     5821 aatttccctt aacttccata tcaaggtctt caatggtgta atgaggtttc aaaaatgaga
     5881 aatctttctt aatcgtgaaa tgattcccat tggctaatgt cacattaaac tttggtaaaa
     5941 aacttaaaac cttcttctca atctgactaa tgagatgttc ctcttcgtca aagactttaa
     6001 aagtcttagg tagagggaac aaacttcctt caacgtgata acagggaagc ccagtcaggt
     6061 ctgtaatagt aaatttaccg cctagagaaa acattttttc tttaatttga aaagtagcca
     6121 tatctcttcc tcctcgaaag ttgacaaata gcaaaagaca agtccattac gaagggcgaa
     6181 aaccgcggta ccaccttcat tcaatgaact tgtcattctc atttattttt agttttaccc
     6241 cattgagtag catctttttc tgattatcca tgactcgcag caaccgtcac ttttctgtat
     6301 ctaatcgttg aaatttcatt ctcaatagta ttaattatac agtcttttta agacttggtc
     6361 aactattttt taagtgatga tgaaccactt gttgtagcat attgtgataa gatgcctgca
     6421 aatgctttat ctttaatctt aacattagct ttttctaagg cttttgaaat cactttattt
     6481 tggaaggctc tatcgcttgt tttatcctta agaataactt cttttaagcg atttttgtaa
     6541 gatttccaat cagatttttt ctctgtctta tcagttactt taatgatgta gtaacttgtt
     6601 ttataagttg ttgaatcaac cgttgaaacc acatctgaaa caccattttt atctagctta
     6661 aaggctgctg acataacttc tttagggagg gttgtccctg cagaatcaaa tttatactca
     6721 actttcttat cagtagctgt tgttttttct tttgcaatct ttgcaaaatc agctccatca
     6781 gcctttacat ctttaaggac agatttagct ttatcttctg catccaattt gattacttgt
     6841 acagaagttt caggagtata gtttttatat gcttctttat agtttgcttc tgttaattct
     6901 ttcttagctg cttcttttac agcatattcc acaagcatag ttgtgcgaat ttgttgtttg
     6961 taaccttccg gagtcaaacc tgcttgtgaa agtgcgcttg aaaatgaatt accatagcct
     7021 ttagctgtct tattataagc ttctgatact tttttatctg aaactttatc accatactgt
     7081 gtatcaaaaa cacgtgagag gatcaatgta agcattgatt gttgtgcagc ttttgatgtt
     7141 tttacttgat cataaaaatc agagactgta atagtgtcgc ccttcattgt aacaacattt
     7201 gttccatttg atgttttacc tgagcaggca gctaatgtgg caactgacat aagtgtcaaa
     7261 aagcctgctg caagttttga tcttgttttc atgatttata actccttttt gtcattaact
     7321 ccattattat atcataattt cttaaaaatg ccttaatctt ctagtacaat atgatctgca
     7381 tttttacgaa tcatcaataa accatctccc aaaggtacca aagtagcagt taaatcagga
     7441 tgttgcaaag tcgagtcaaa taaacgttgc agacctcggt agatagttcg ctgacctctt
     7501 ctcacttcat cgataggttt tgcaatatct ccaccttgga aaatatcatc taaaacaact
     7561 actcctccaa catctaaatg ttttaatact tgaggtaaaa aaacgatata tttagactta
     7621 gcagaatcca tgaatacaaa atcgtaagat ttatccaatg tttgtaacac atccacagca
     7681 tcgccttcca aaagggttat ctgattgtga ttatcatatt ttgcaaagtt ttccttagcc
     7741 aaggcaatca tctcttcatt acgatcgatc gttgttatct ttgcttcagg agcgttttct
     7801 gccatcaaaa gggccgaaaa accaatagct gttccaattt ctaaaatgtg cttaggttgt
     7861 aatgtttgca tcaaaaaacg aaaataagtc gctgtttcat gaggtataat agggatattt
     7921 tcctgtttag caaatgcttc taactctgct aaacagccat tattttgttt ttggtgctga
     7981 cgcatatatt gaacaatttc ttctttaact actggacgac gcatattatg attagcattt
     8041 ttactgtaag attgaaccac ttttggctcc tcttttctat tttaaataat aactttttta
     8101 ttatagcaaa aaatgtctaa ctctgagaat gatttaatcc aaacgagtga aatcaagtga
     8161 tattatatct ttgagacttg aaatattata gttttctttg gaattctcta accttaagtt
     8221 tatggatttt ataccagcat tttgagcaac ctccaaatct agtggacgat ctcctatgta
     8281 ataagtcatt gatttatcta aagaatatcg tttaactaaa taattaatcc cttgtggatg
     8341 tggttttcgc tcgaatcccg aaacaccagt taaaatttca tcaaaataat gagagatctg
     8401 caaggtttcc aacactgaat gcgtacttgc tcctttatgt gtatacataa aattggggat
     8461 atcttgttct ttggtccatt ctaaaatctc ttttgcatat ggcattaaat gtattttaga
     8521 atctcgactt tcttgttctt ttgtaaaata tgctttcagt ttttcatgag gtatttgctc
     8581 ttcctctgaa aggtttacca ataatttccc cactgattcc tgtaaaatat attcatggat
     8641 taattcttta tcaaatatta aaccaaaatg acgataggtt tcttcaagag cttccataat
     8701 tggtacatac gaatctatta atgtcccatc taaatcccaa ataaaagtaa gcttcttcat
     8761 cctatttccc ttcttttttg acaatagcag ttttggtctc ataaaaatca taacttgggt
     8821 aggtatttcc acctattgag ttttccacta actaacttta ccaaaaccac tctcaaaaca
     8881 aactataaaa taactgatca ctatcaaaaa ccagtctagt tgactggttt tacttcaata
     8941 gctatatttt caacttaatc attatggacg ccttttgcta caagattttc cagttccact
     9001 aaacgctctt caaatactct aaaagctgca tccagatagt ctgcactagt catatcaaca
     9061 ccagctttag caatgacgtt tagagggtaa tctgaattcc cagctttaag gtaatttagg
     9121 taggcctctt tatcctccgg attaccgttt acaatacgct ctgctaaata atttgctgct
     9181 gcgaagccgg ttgcatattg gaaaacataa taattgtagt aaaagtgtgg aattcttgcc
     9241 cattcatatt gaataaaatg attatcttct ttagtcaaac cataatattt ttcattaagc
     9301 tcagcgtaaa gattgtttaa gtattcgcta gttagtactt gcccctcctg atcagctaca
     9361 tgaatggcat gttcgaattc agcgaactgt gtttgcctaa aaattgttcc tttgaaaccg
     9421 tccaaataat gattgagaat tgcaaagcga tttttatcat ctttaacttc tttcaaaagg
     9481 gtttctgtta atatattttc atttgttgtt gatgcaattt cagctaggaa aatagaataa
     9541 tcaccataaa catagggttg attttcacga gtaaacgtcg agtgtagact atgtccagtt
     9601 tcatgcacca gcgtataaag attatctaaa gtatcttgcc agttaagaag catgaaagca
     9661 ttagtgtcat aagatccacc tgaataagcg cctgacctct ttcctttgtt aacatggaca
     9721 tcaatccaac gttcagtaaa agcacgatga acacgttcag aatatgcttc gccaaaaata
     9781 gctagaacct cctctgattt ttttagagct tcatcataag taaaactcat atccatttga
     9841 gaaaggggtg tatatacgtc atacatcttc aaatcatcta gcccaagaac tttttgacgc
     9901 aacttcatat aacgatgtaa gagcggtaaa tgatgattaa ctgtcttaat tagagtttcg
     9961 tagacctctt ctggaataaa attggcagat agagctgatt ggcgtgctga ttgataatga
    10021 tgcacacgag ccttaaaatt ttgagatttc acatttgttt gtaatgtttt agcgtaagta
    10081 tgctggaatt gttcatatgt gctatacata gcttgatatg cttcttttcg aaccgtccta
    10141 tcgctagatt ccataaggga aatgaaatta ccatgtgtaa gctcaacttc ctcacctttt
    10201 gcatttttta caactgggaa gaccatatct gcattatcaa ggatttcaaa tgtctcacca
    10261 gctgctccaa aaatctcact agcaccagct aacaattctt cttcgttttg agataagaca
    10321 tgaggtttat ttgcaaatat tttctcaaag aaatgatcat atttttgcaa gtcaggcatt
    10381 tctaaaagga atgactggta atcagactct gaaagttgca atagttctgg ctcgtaaaac
    10441 gaaaatgttt cactaaattt tgcatagagc gctgtcgctt tcgcttgaaa ttcttgatat
    10501 ttcgcaacag tggtgtcttg gtcatttttc attgaagcat agacataaac tttttctaaa
    10561 cggcgagata aatctaattc aacctccgtg atctccaata aagattggct tgagtccaac
    10621 agatgacctg aaaaaccctt tgcattatcg atggcttgag taagctctac cacttcagtt
    10681 tcccataact catcagtcgc aaagacagta gttaaatccc attggtattt ttcttcaata
    10741 tgtgagcgat tgtctgacat tttgtatcct ccagtttaat tcttagtgac tattttatca
    10801 taaaatacag ggggataaag tgtttggtct aagttattct tatttttttg ataataattt
    10861 gtaaaattct gataaaacga tgttagagaa tttgttattt gacaaaaatc agtatctaca
    10921 cgtggtggct gtatttgagg aaaaaacatc attagctctt gggtcaatag attctgacca
    10981 gaaaggtagg ccttttcttg ttttcgtaac caaaattgat tgccatagcg taactgctga
    11041 cgcacataat ctctgatatt tctgtcttgt ttaa
//
LOCUS       DATRMA010000063        10908 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.063, whole genome shotgun sequence.
ACCESSION   DATRMA010000063 DATRMA010000000
VERSION     DATRMA010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10908
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.063"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            complement(<1..598)
                     /locus_tag="VBC28_001555"
     CDS             complement(<1..598)
                     /locus_tag="VBC28_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEO6815527.1"
                     /translation="MNHQILQNYISSHNLPIIEKDYHKYLTFESLEEDYTYILKDGIV
                     KQSVLSKYGTEFNLRYVTGLEITSILNTDYSQHMGEPYNVRIESETAHFYKVRRSTFL
                     KDINNDIELQGYVKDFYHNRLEKSMKKMQCMLTNGRIGAISTQLYDLSKMFGEERDNG
                     DIYINFVITNEELGKFCGISTGSSVSRILKQLKDDHIIR"
     gene            755..1957
                     /locus_tag="VBC28_001556"
     CDS             755..1957
                     /locus_tag="VBC28_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO6815528.1"
                     /translation="MEDKLFNKHFIGITILNFIVYMVYYLFTVIIAFIATKELGVSTS
                     QAGLATGIYIVGTLIARLIFGKQLEVLGRKLVLRGGAIFYLLTTLAYFYMPSIGVMYL
                     VRFLNGFGYGVVSTATNTIVTAYIPADKRGEGINFYGLSTSLAAAIGPFVGTFMLDNL
                     HINFKMVIVLCSILIAIVVLGAFVFPVKNITLNPEQLAKSKSWTIDSFIEKKAIFITI
                     IAFLMGISYASVLGFQKLYTTEINLMTVGAYFFIVYALVITLTRPSMGRLMDAKGDKW
                     VLYPSYLFLTLGLALLGSAMGSVTYLLSGALIGFGYGTFMSCGQAASIKGVEEHRFNT
                     AMSTYMIGLDLGLGAGPYILGLVKDGFLGAGVQSFRELFWIAAIIPVVCGILYFLKSS
                     RQVETKTI"
     gene            1975..2427
                     /locus_tag="VBC28_001557"
     CDS             1975..2427
                     /locus_tag="VBC28_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEO6815529.1"
                     /translation="MTQKYERILIAIDGSYESELAVEKGINVALRNDAELLLTHVIDA
                     HAYQSEGVFSDYVFDRQEQESADVLAYFEKLAHSKGLTKIKKITEIGNPKTLLAKDIP
                     IREKADLIMVGATGLNTFERLLIGSTSEYILRHSKVDMLVVRDSKKTL"
     gene            2595..3443
                     /locus_tag="VBC28_001558"
     CDS             2595..3443
                     /locus_tag="VBC28_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000570237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEO6815530.1"
                     /translation="MIEITWTVKYITEFIATAFLIILGNGAVANVDLKGTKGNNSGWI
                     IIAIGYGLGVMMPALMFGNVSGNHINPAFTLGLAFSGLFPWAHVGQYILAQILGAMFG
                     QLVVVMVYQPYFVKTENPNHVLGSFSTISALDDGQKSSRKAAYINGFLNEFVGSFVLF
                     FGALALTKNYFGVELVGKLVQAGYDQTTAATRISPYVTGSLAVAHLGIGFLVMTLVAS
                     LGGPTGPALNPARDLGPRIVHRLLPKQILGQAKEDSKWWYAWVPVLAPIVASILAVAL
                     FKLLYL"
     gene            complement(3522..4418)
                     /locus_tag="VBC28_001559"
     CDS             complement(3522..4418)
                     /locus_tag="VBC28_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEO6815531.1"
                     /translation="MNETLLLHGIQLILIIAMIITFYQIVRHIRSQKINPFKRFFTGL
                     WIGFVTDALDTLGIGSFATTTTFFKLTKLVEDDRKIPATMTAAHVLPVLLQSLCFIFV
                     VKVEALTLITMAGAAFIGAFVGAKMTKNWHAPTVQRILGTLLITAAIIMLYRMITNPG
                     AGISDSVHGLHGIWLFVGIGFNFIIGVLMTMGLGNYAPELIFFSLMGLSPAVAMPVMM
                     LDAAMIMTASSTQFIKSGRVNWNGFAGLVTGGILGVIVAVLFLTNLDLNSLKTLVVGI
                     VLFTGAMLIRSSFKKTMPKSHH"
     gene            4543..5298
                     /locus_tag="VBC28_001560"
     CDS             4543..5298
                     /locus_tag="VBC28_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CppA family protein"
                     /protein_id="HEO6815532.1"
                     /translation="MTLFHSLTFKHPVLRVNNRDLNIAFYQESLGFKLISEENAIAVF
                     SAWQNKEASFIIEESPTYRTRAVNGTKKLAKIIVKSQDAKDIEKLLANGAQAIQVYQG
                     QNGYAYETVSPEGDLFLLHAEDDLSQLVAIERPELEKKDDTTGLSNFAFQSISLNVPD
                     AVKAEAFYDKVFAGKFPINLSFKEAQGQDLQIAPNETWDIEILECCVNEDTNLNDLKS
                     TFESLGLDVYLDSKEKILVISDTSNIEIWISKE"
     gene            5295..6224
                     /locus_tag="VBC28_001561"
     CDS             5295..6224
                     /locus_tag="VBC28_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HEO6815533.1"
                     /translation="MIKTNTVLELIKEQIKDNLYHGASLAIYENGEWHEHYLGTIDGN
                     EKVKAGLVYDLASVSKVVGVGTLLAKLVYQGTIDIDKPLRYYYPTFHHQTLTVRQLAT
                     HSSGIDPFIPNRDQLNATQLKDAINHIKVLEDKSFKYTDINFLLLGFMLEEVLGDSLD
                     KLFKRYIFTPFQMKETSFGPRVEAVPTVAGINDGIVHDPKAKVLGKHTGSAGLFSTID
                     DLQRFSIHYLKDDFAKPLWNNYSLSKSRSLAWDIDKDWINHTGYTGPFIALNYQKQAA
                     AIFLTNRTFSYDDRPLWIKKRRHVQEAITSYFE"
     gene            complement(6259..6633)
                     /locus_tag="VBC28_001562"
     CDS             complement(6259..6633)
                     /locus_tag="VBC28_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000931877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEO6815534.1"
                     /translation="MLNHKFLQVLKYEGVVSITSWIELAPHVTNTWNSYLTITDDQRI
                     LAPAAGMTHLENDLNNNSKIIMTLGSREVEGRDGYQGTGFRIEGTAKLLEAGSDFEIV
                     KEKYPFLRKVLEVTPINVIQLL"
     gene            complement(6734..9046)
                     /gene="pflB"
                     /locus_tag="VBC28_001563"
     CDS             complement(6734..9046)
                     /gene="pflB"
                     /locus_tag="VBC28_001563"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HEO6815535.1"
                     /translation="MATVKTNTDIFEQAWEGFKGVDWKEKASIARFVQANYAPYDGDE
                     SFLAGATERSLHIKKVIEETKAHYEETRFPMDTRVASISELPAGFIDKDNELIFGIQN
                     DELFKLNFMPKGGIRMAETTLKENGYEPDPAVHEIFTKYATTVNDGIFRAYTSNIRRA
                     RHAHTVTGLPDAYSRGRIIGVYARLAVYGADYLMQEKVNDWNALNDIDEESIRLREEI
                     NLQYQALGEVVKLGDLYGVDVRKPAMNTKEAIQWVNIAFMAVCRVINGAATSLGRVPI
                     VLDIFAERDLARGTFTESEIQEFVDDFVLKLRTVKFARTKAYDALYSGDPTFITTSMA
                     GMGADGRHRVTKMDYRFLNTLDNIGNSPEPNLTVLWSDQLPYAFRRYCMSMSHKHSSI
                     QYEGVSTMAKEGYGEMSCISCCVSPLDPENEDKRHNLQYFGARVNVMKALLTGLNGGY
                     DDVHKDYKVFDIDPIRDEVLNFDTVKANFEKSLDWLTDTYVDAMNIIHYMTDKYNYEA
                     VQMAFLPSHVRANMGFGICGFANTVDSLSAIKYATVKPIRDEDGYIYDYETVGDFPRY
                     GEDDDRVDSIAEWLLEAFHGRLAKHKLYKDAEATVSLLTITSNVAYSKQTGNSPVHKG
                     VYLNEDGSVNLSKVEFFSPGANPSNKAKGGWLQNLNSLSKLDFAHANDGISLTTQVSP
                     RALGKTFDEQVDNLVTVLDGYFENGGQHVNLNVMDLKDVYDKIMNGEDVIVRISGYCV
                     NTKYLTPEQKTELTQRVFHEVLSMDDALTN"
     gene            9216..10325
                     /gene="dinB"
                     /locus_tag="VBC28_001564"
     CDS             9216..10325
                     /gene="dinB"
                     /locus_tag="VBC28_001564"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000504687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEO6815536.1"
                     /translation="MGERTMLIFPLINDTSRKIIHIDMDAFFASVEERDNPSLKGKPV
                     IIGSDPRKTGGRGVVSTCNYEARKFGVHSAMSSKEAYERCPQAIFISGNYQKYRQVGM
                     EVRDIFKKYTDLVEPMSIDEAYLDVTENKMGIKSAVKLAKMIQYDIWNDVHLTCSAGI
                     SYNKFLAKLASDFEKPKGLTLILPDQAQDFLKPLPIEKFHGVGKRSVEKLHALGVYTG
                     EDLLSLSEISLIDMFGRFGYDLYRKARGINASPVKPDRVRKSIGSEKTYGKLLYNEAD
                     IKAEISKNVQRVVASLEKNKKVGKTIVLKVRYADFETLTKRMTLEEYTQDFQIIDQVA
                     KAIFDTLEESVFGIRLLGVTVTTLENEHEAIYLDF"
     gene            complement(10396..10878)
                     /locus_tag="VBC28_001565"
     CDS             complement(10396..10878)
                     /locus_tag="VBC28_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000255479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6815537.1"
                     /translation="MVSYEKVRRSLRTATITIIVLNSLSLVFRLFTGISVQLAKTEIN
                     KGNTGNLPKEHIEAVLSATTPFMLFVTALIVLVNIAIVIFCIKNLRAIKRNQMVNYLP
                     YYLGFAITVGLVILGFLTTKAPWAIAINIVFQAIFGLLYFHAYQKAQKLNERDLEETN
                     "
BASE COUNT         3443 a         1801 c         2117 g         3547 t
ORIGIN      
        1 tacgaataat atgatcgtct tttagttgtt tcaaaatgcg gctcacgcta cttcctgtgg
       61 atattccgca aaatttacca agttcttcgt tcgtgataac aaaattaata tagatatcac
      121 cgttatctcg ttcttcacca aacatcttac tgaggtcata taattgtgtg gagatagcac
      181 caattcgacc attagtgagc atacattgca tttttttcat agatttttca aggcggttat
      241 gatagaaatc tttcacatat ccttgcagtt caatatcatt attgatatct tttaggaagg
      301 tagagcgacg tactttgtaa aagtgggcag tttcggattc gattcgaacg ttgtaaggtt
      361 ctcccatgtg ttgggagtag tctgtattta ggatagaagt gatttctaag cctgtcacat
      421 aacgcagatt gaactcagta ccatatttag agagaacact ttgtttaaca ataccatctt
      481 ttaggatata agtatagtct tcttctaaac tttcaaatgt aaggtattta tggtagtctt
      541 tttcgataat tggaagatta tgactactaa tgtaattttg taaaatttga tgattcatga
      601 cttcctccaa atgttttagg tttgatagac caccttaaca tttgttaatg cagtctgata
      661 ctagcactta tattatacca ttgaaatatc gtatcttcta atatttttgt aaaatgttta
      721 tgaaaatgaa attgaaaaaa aggagccatt tgcattggaa gacaaattat tcaacaaaca
      781 ttttataggc attactattt taaactttat tgtttatatg gtctattatt tgtttaccgt
      841 tatcatagct tttattgcga ctaaagagtt aggtgttagc actagccaag caggattagc
      901 aacggggatt tatattgtag ggactttgat tgctcgtctt atatttggca agcaattaga
      961 agttctagga cgtaagttag ttttacgtgg aggggctatt ttttacttac taacaacttt
     1021 agcttatttt tatatgccaa gtatcggagt aatgtattta gttcgtttcc taaatggttt
     1081 tggttatggc gtcgtgtcaa cagcaactaa tactattgta acagcctata taccagctga
     1141 taaaagaggt gaggggatta acttttacgg tctatcaaca agtttagccg cagctattgg
     1201 tccttttgta ggaacattta tgctagacaa ccttcatatt aactttaaaa tggttattgt
     1261 attatgtagt attttaattg cgattgtagt gttgggagca tttgttttcc cagtcaaaaa
     1321 tattacttta aatccagaac agttagctaa atcaaaatca tggactattg atagtttcat
     1381 tgagaaaaaa gcaattttta tcacaattat tgcatttttg atgggtatct cctatgcttc
     1441 cgtgttaggt ttccaaaaat tatatacaac agaaattaat ttgatgacag taggagctta
     1501 tttctttatt gtttatgcac ttgtcatcac tttaaccaga ccatctatgg gaagattaat
     1561 ggacgctaag ggagataagt gggtgcttta tccaagttat ctgttcttaa ctttgggact
     1621 tgctttatta gggagtgcta tgggaagtgt tacctacctt ctatcaggtg ctttgattgg
     1681 ttttggttat ggcaccttta tgtcttgtgg ccaagcagca tcaatcaaag gtgttgagga
     1741 acatcgtttc aatacagcca tgtcaactta catgataggt cttgatttag ggttaggtgc
     1801 tggaccttac attttgggac ttgttaaaga tggttttctt ggagctggtg tgcaatcctt
     1861 tagagaatta ttctggatag cagcgattat tcctgttgtt tgtggtattc tatatttctt
     1921 aaaatcatct agacaagttg aaactaaaac tatataaaag ggaggaatta agctatgaca
     1981 caaaaatatg agcgtattct tattgctatt gatgggtctt acgagtcaga attggcggta
     2041 gaaaaaggga taaatgttgc tcttcgtaat gatgctgaat tactgctaac gcatgttata
     2101 gatgctcacg cttaccaaag tgagggagtc tttagtgact atgtttttga taggcaagaa
     2161 caagagtcag cagatgtgct agcctatttt gagaaattag cacattcaaa aggccttaca
     2221 aagattaaaa aaattactga aatcggaaat cctaagactc ttttggcaaa ggatatcccc
     2281 ataagggaaa aagcagattt aattatggta ggcgcaacag gattaaacac ttttgaaaga
     2341 ctattgatcg gctcaacttc agaatacatt ttacgtcatt cgaaagttga tatgctagtc
     2401 gttcgtgata gtaagaaaac cttataaact aaataaaata gagtactagg tagtccgaac
     2461 ctagtactct attttttatc aaatatattt ggttaaaaat tttattaatt ttattttgtg
     2521 ataagcttta caatattaag cttacttggt agaatggtta atgttgtaaa tatttttttg
     2581 ggaggaaaca aggattgatc gaaatcactt ggactgtgaa gtatatcaca gaatttatcg
     2641 caaccgcttt tcttatcatc ttaggaaatg gggctgtagc taacgttgac ttaaaaggca
     2701 caaaaggaaa taattcaggt tggattatta ttgcaattgg ttatggttta ggtgttatga
     2761 tgccagcgct tatgtttggt aatgtctctg gaaaccatat taacccagct ttcacacttg
     2821 gattggcatt ttcaggtctt ttcccttggg cacacgttgg acaatacatt cttgcacaaa
     2881 ttttaggagc aatgtttgga cagttagttg tcgttatggt ttatcaacct tactttgtta
     2941 agacagaaaa tccaaatcat gttcttggat cattttcaac tatttctgca ttagatgatg
     3001 gacagaaatc aagtcgtaaa gcggcttaca ttaatggttt cttaaatgaa ttcgttggtt
     3061 cttttgttct tttctttgga gctttggctt taacgaagaa ctattttggt gtagaacttg
     3121 ttggtaaact tgttcaagct ggttatgacc aaacgacagc agcgacaagg atttcaccat
     3181 acgtaactgg ttcacttgct gtagctcact taggtattgg tttcttggta atgactctag
     3241 ttgcttcttt aggtggaccg actggacctg cattgaaccc tgcgcgtgac cttggtccac
     3301 gtattgttca ccgcttatta ccaaaacaaa ttcttggaca agctaaagaa gattcaaaat
     3361 ggtggtatgc atgggtgcca gttttagcac caatcgtagc aagtatactc gctgtagcct
     3421 tgttcaaact attatatctt taatagttaa agaaagatcg gtttaccggt ctttttttgt
     3481 tttaaatata aaaatcagcc agtcttgcta gctgatttcg tttaatgatg acttttgggc
     3541 atagtttttt taaaggaaga acgtattaac attgctccag taaataaaac aatacctaca
     3601 accagtgttt tcaagctgtt aagatcaagg ttagttaaga agagaacagc gacaataacg
     3661 cctaatattc ccccagtaac cagaccggca aagccgttcc aattaacacg accactcttg
     3721 ataaattgtg tactagaagc tgtcattatc atagctgcat ctagcatcat gacaggcata
     3781 gcaactgcag gactcagtcc cattagggag aagaagataa gttcgggagc ataattacct
     3841 agtcccatag tcattaatac accaataata aaattgaagc cgataccaac aaataaccaa
     3901 atgccgtgta gaccatgcac tgaatctgaa ataccagcgc cagggttagt gatcatacga
     3961 tagagcataa taatagcagc tgtgatgagc agtgttccta gaatacgttg aacagtagga
     4021 gcgtgccaat ttttagtcat tttagctcca acaaaagccc caataaaagc tgcaccagcc
     4081 attgttatta gtgttaaagc ctctactttt acaacaaaaa taaaacagag tgactgcaac
     4141 aaaactggaa gtacgtgtgc agctgtcatt gttgcgggta tcttacgatc gtcttcaaca
     4201 agcttagtta gtttaaaaaa tgtggtcgtt gttgcaaaag agccgatacc aagagtatcg
     4261 agagcatcag ttacaaaacc gatccacaaa ccagtgaaaa atcgtttgaa aggatttatt
     4321 ttttgagacc taatatgtct aacaatctga taaaaagtga taatcatggc gataatcaga
     4381 ataagctgaa tcccatgtaa tagtagtgtt tcattcataa tatccagtct acaaaaaaat
     4441 agtgaaactg tcaatttgac tttatggtat tgagagcaca aagattcttg ctaaattatc
     4501 taaaattatc taaaatagac tagactgaac aaggaggaat ttatgacgtt atttcattca
     4561 ttgacattta aacacccagt attacgtgtt aataatagag acttgaatat tgcattttat
     4621 caggagtctc ttggatttaa attgataagt gaagaaaatg caatcgcagt tttctcagca
     4681 tggcagaaca aagaggctag ctttattatt gaggaatccc caacttatcg tactcgtgcg
     4741 gtgaatggta ctaagaagtt agcaaagatt attgttaagt ctcaagatgc aaaagatatt
     4801 gaaaagcttt tggcaaatgg tgcgcaagcc attcaagtat atcaaggtca aaatggttat
     4861 gcctatgaaa cagtttcgcc cgagggagac cttttcttac ttcatgctga agatgatttg
     4921 tcacaattag tggcaattga gcgcccagag ttagaaaaaa aagatgacac tacaggtcta
     4981 tcaaactttg ctttccaatc tattagtttg aatgtacctg atgccgtgaa agctgaagct
     5041 ttttacgata aagtatttgc tgggaagttc ccaattaatt tatctttcaa agaagcacag
     5101 ggacaagatt tgcaaattgc tccaaatgaa acttgggata ttgagatact agaatgctgt
     5161 gttaatgaag atactaattt aaatgacttg aaatcaactt ttgaatcatt gggacttgat
     5221 gtttaccttg attctaaaga aaagatttta gtcatctctg acactagtaa tattgagatt
     5281 tggatttcaa aagaatgatt aaaactaata ctgttttaga actcattaag gagcaaatca
     5341 aagataatct ttatcatggt gcaagcttag ctatttatga aaatggagag tggcacgaac
     5401 attacttggg gacaattgat ggcaatgaga aagttaaagc gggattggtc tatgatctag
     5461 ccagtgtatc aaaagttgtc ggcgtgggaa ctttactagc aaagttagtt tatcaaggga
     5521 ctattgatat tgataagcca ttaaggtatt attaccctac gtttcatcat cagacgttaa
     5581 ctgtaaggca attagctaca catagcagtg gaattgaccc ttttattcca aatcgtgatc
     5641 agttgaatgc tactcagctg aaagatgcca ttaatcatat caaggttttg gaagataaat
     5701 cgtttaaata cactgatatt aattttcttt tacttggttt tatgttagaa gaggtattag
     5761 gggatagttt ggataaactt ttcaaacgtt atattttcac tccttttcag atgaaagaaa
     5821 cctcttttgg tcctagagta gaagctgttc caactgtagc cggtattaac gatggtattg
     5881 tacacgatcc taaagctaag gtattaggaa agcatactgg ttctgcaggt ttattttcaa
     5941 ctattgatga tttacaaaga ttttctattc attatttaaa agatgatttt gcgaagcctt
     6001 tgtggaataa ttatagtttg agtaaatcaa gatcattagc ttgggatata gataaggatt
     6061 ggataaacca tactggttat actggtcctt ttattgcact aaattatcaa aagcaggcag
     6121 cagctatttt tttgacaaac agaacctttt cctatgatga ccgtccactt tggattaaga
     6181 aacgacgaca tgtacaagag gcgataacat cgtactttga ataaaaaact tctcctagaa
     6241 ttttctagga gaagtttttt ataaaagttg gataacattt atgggagtta cttccaatac
     6301 tttacgcaga aaagggtatt tttctttaac aatttcaaaa tcgcttcctg cttctaatag
     6361 cttagcagtt ccttcaattc taaagcctgt accttgataa ccatcacgac cctcgacttc
     6421 acggctgccc agagtcataa taattttaga attattattg agatcatttt caagatgagt
     6481 cattcctgct gcaggagcta atatacgctg gtcatctgta atagtcaggt agctattcca
     6541 agtgtttgta acgtgaggag ctaattcgat ccagcttgtt atcgaaacga caccctcata
     6601 ttttagaact tgaagaaatt tgtgatttaa cattaggccc ctccttgagg ttatataaag
     6661 aagtaattag tactattgat aatatagata aaagtaggtt tgatataatc aaacctactt
     6721 catcgaatac aaactagtta gttaatgcat cgtccattga aagtacttca tggaagacac
     6781 gttgtgtcaa ctcagttttt tgctcaggag ttagatattt agtattgaca cagtaacctg
     6841 agatacgaac gataacatct tcaccattca taatcttgtc atagacatct ttaaggtcca
     6901 tgacattcaa gttaacgtgt tgaccaccgt tttcaaagta accatcaagg actgttacca
     6961 agttatcaac ttgttcatca aatgttttac caagagcgcg tggagaaact tgagttgtca
     7021 atgaaatacc atcatttgca tgagcgaaat caagtttaga aagtgaattc aagttttgta
     7081 accagccacc tttagctttg ttagaagggt tagcacctgg tgagaagaat tctactttag
     7141 aaaggtttac actaccatct tcattgagat atactccttt atgtaccggt gagttaccag
     7201 tttgtttaga gtaagcaacg tttgatgtga ttgtaagaag agaaacagtt gcttcagcat
     7261 ctttgtaaag tttgtgttta gcaaggcgtc catggaaggc ttcaagtaac cattcagcga
     7321 ttgagtcaac acgatcatca tcttcaccat aacgtgggaa gtcaccaact gtttcgtagt
     7381 cgtagatgta accatcttcg tcacggattg gtttaacagt agcgtattta atcgcagata
     7441 atgaatcgac agtatttgca aaaccacaga taccaaatcc catattagca cgtacatgtg
     7501 aaggtaagaa tgccatttgt acagcttcat agttgtattt atcagtcatg tagtggatga
     7561 tattcatcgc atcaacataa gtatcagtca accagtcaag agatttttca aagttagctt
     7621 ttacagtgtc aaagttgagt acttcgtcac gaattgggtc gatgtcaaat actttgtaat
     7681 ctttatgaac atcatcataa ccaccgttaa gaccagtaag aagtgctttc ataacgttaa
     7741 cacgagcacc aaaatattga aggttgtgac gtttgtcttc gttttctgga tcaagtggag
     7801 atacacaaca tgaaatacat gacatttcac cgtagccttc tttagccatt gttgatacac
     7861 cttcatattg gattgaagag tgtttatgac tcattgacat acagtagcga cggaaagcat
     7921 atggtaattg atctgaccaa agaacagtta agtttggttc tggtgagtta ccgatattgt
     7981 caagtgtatt taagaaacga tagtccattt tggtaacacg atgacgtcca tcagctccca
     8041 tacctgccat tgaagttgtg ataaaggttg ggtcacctga gtaaagggca tcataagctt
     8101 tagtacgagc aaattttaca gtacggagtt taaggacaaa atcatcaaca aattcttgga
     8161 tttctgattc tgtgaatgta ccgcgagcga ggtcacgttc tgcaaagata tcaaggacaa
     8221 taggaacacg tccaagtgat gttgcagcac cattgataac acgacataca gccatgaaag
     8281 caatgttcac ccattgaatg gcttctttag tgttcatggc tggtttacga acatctacgc
     8341 cgtaaaggtc accaagtttt acaacttcac caagagcttg gtattgaagg ttgatttctt
     8401 cacgaagacg aattgactct tcatcaatat cattcaatgc attccagtca ttaacttttt
     8461 cttgcatcaa gtagtcagca ccgtaaacag ctagacgagc atatacacca atgatacgtc
     8521 cgcgtgaata agcgtctgga agaccagtaa cagtgtgtgc atggcgagca cgacgaatgt
     8581 tagaagtata agcgcggaag ataccatcat ttactgttgt agcgtactta gtgaagattt
     8641 cgtgaacagc tggatcaggt tcataaccat tttctttaag tgtagtttcc gccatacgga
     8701 taccaccttt aggcatgaag ttcaatttga aaagctcgtc gttttgaata ccaaagataa
     8761 gctcgttatc tttgtcgata aaaccagctg gtaattcaga aattgaagca acacgagtat
     8821 ccattgggaa gcgagtttct tcatagtgag cttttgtttc ttcaatcact tttttgatgt
     8881 gaagtgaacg ttcagttgct ccagctaaga agctttcatc tccatcgtaa ggtgcatagt
     8941 ttgcttgaac gaagcgagca atacttgctt tttctttcca atcaacacct ttaaagcctt
     9001 cccaagcttg ttcaaagata tctgtgtttg ttttaactgt tgccatagtt ttctccttaa
     9061 acattgtgac agatttaatt ttgtttacat atccatttta ggaaaaagta aacgttttcg
     9121 caagcatatt tcttgacaaa attatgattc ttttataata cagttttttt aatctgtatc
     9181 attaagttgt aatctgttat aatggagtag agagaatggg ggagagaaca atgttaattt
     9241 ttcctttaat aaatgatacg tcgcgtaaaa ttatccacat tgatatggat gctttttttg
     9301 catctgtgga agagcgtgac aatccctctc ttaaaggaaa gcctgttatt attggttctg
     9361 atcccagaaa aaccggcggc agaggggttg tttctacttg taattatgag gctcgaaagt
     9421 ttggagttca ttcagctatg tcttcaaaag aagcgtatga acgatgccct caagctatct
     9481 ttatttcggg aaattaccaa aaatatcgcc aagtaggaat ggaagtacga gatatattta
     9541 aaaagtatac ggatttggtt gaaccgatga gtatagacga agcttattta gatgtaactg
     9601 aaaataaaat gggaataaaa tcagctgtta aacttgctaa aatgatacaa tacgatattt
     9661 ggaatgatgt ccatttgacc tgttcagcgg gtatttccta caataaattt ttggcaaaat
     9721 tggcttctga ctttgagaaa ccaaaaggac ttaccctcat cctaccagat caagctcaag
     9781 atttcttgaa gcccttacca atagagaaat ttcatggtgt tggtaaacgc tccgtcgaaa
     9841 aattacatgc tttaggggtt tatactggtg aggacctcct ttccttatct gaaatttctt
     9901 taattgatat gtttggtcgt ttcgggtatg acctttatcg taaggcaaga gggattaatg
     9961 cttccccggt aaaacctgat agagttcgta aatcaatagg aagtgagaaa acctatggga
    10021 aattactata taacgaagca gatatcaaag ctgagatttc aaagaacgtt cagagagttg
    10081 ttgcttctct ggagaaaaat aagaaagttg gtaagacaat cgttttaaaa gttagatatg
    10141 cggattttga aacattgacg aaacgtatga cattagaaga gtatactcaa gattttcaaa
    10201 tcattgatca agttgctaaa gcgatatttg ataccttaga agagtcagtt tttggcatac
    10261 gactcttagg tgttacagta acgaccctgg aaaatgaaca tgaagctatc tatttagatt
    10321 tttaaaagtt gggacgatga ctatatttaa ctctctttga aaagaaaaaa gcactagatt
    10381 ctagtgcttt tacttttagt tagtctcttc aagatcacgt tcgttgagtt tttgtgcttt
    10441 ttgataagca tggaaataaa gaagaccgaa aatagcttgg aagacgatat ttatagctat
    10501 tgcccatggt gctttagtag ttagaaaacc caaaataaca agtcctacag taatggcgaa
    10561 gccaaggtaa taaggaaggt aattaaccat ttgatttctt ttaattgctc ttaaattttt
    10621 aatacagaaa ataacaatgg caatgttgac taatactatt aaagcagtga caaatagcat
    10681 aaatggtgtt gtggcgctca agactgcttc gatatgttct ttaggaagat tacctgtgtt
    10741 ccctttatta atttcagttt tggctagctg cactgaaatt cctgtaaaca atctgaatac
    10801 taaagacaaa ctatttaata caatgatagt gattgtagct gtgcgcagag agcggcggac
    10861 tttttcatat gaaaccataa tatctccttt ttctaaataa accttaat
//
LOCUS       DATRMA010000064        10680 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.064, whole genome shotgun sequence.
ACCESSION   DATRMA010000064 DATRMA010000000
VERSION     DATRMA010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10680
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.064"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            <1..388
                     /locus_tag="VBC28_001566"
     CDS             <1..388
                     /locus_tag="VBC28_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001086432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO6815538.1"
                     /translation="DDTYRKNERIRDLIDYINNNYQQNLTIEFLADYMGYSKTHFMTV
                     FKQHTGTSCTEFIIQVRLNKASEHLINSTTAIIDIANSVGFNNLSNFNRQFKRYYHTT
                     PRQYRKQFTNKKDGSTLVYRNINTKK"
     gene            complement(445..1977)
                     /locus_tag="VBC28_001567"
     CDS             complement(445..1977)
                     /locus_tag="VBC28_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HEO6815539.1"
                     /translation="MENHKNEFQFSLESILGFVWRGIVVGLISGFVVSIFRLAIEKIF
                     LVVMELYKSAHYQPIILLSITVTSIIAAVIIGFFIKSDPDIKGSGIPHVEGELKGMLS
                     PDWFSIVWKKFIAGILAISSGLMLGREGPSIQLGAMTGKGIAQYLNASRMEKRVLIAS
                     GAAAGLSAAFNAPIAGLLFVVEEIYHHFSRLVWITALVASLVANFVSLNIFGLTPVLA
                     LPSELPSLNLNFYWIFLLMGLFLGILGFIYEWVILRFHVIYDYLGKLFHLPSHLYGIL
                     AVIFILPIGYYFPQLLGGGNGLIVSLPRSNLSLMMLGLFFLIRFLWSMLSYSSGLPGG
                     IFLPILALGSLAGAFFAVGMQYFGIISHQQISLFVVLGMAGYFGAISKAPLTAMILVT
                     EMVGDLKQLMAIGIVTMVSYIVMDLLKGEPIYEAMLAKMTFNPKDKVMTPTLIELTVS
                     DKISGKYVRDLELPENVLITTQIHHKTSAVVSGNTILNAGDTIFLVVNESEIKEVREQ
                     LM"
     gene            complement(2086..3159)
                     /locus_tag="VBC28_001568"
     CDS             complement(2086..3159)
                     /locus_tag="VBC28_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEO6815540.1"
                     /translation="MRRVYSFLGGIILVILILFGLTTYLEKKSSSTPNSDKLVIYNWG
                     DYIDPALLKKFTKETGIEVQYETFDSNEAMHTKIKQGGTTYDIAVPSDYMIDKMIKEN
                     LLVKLDHSKIANWDAIGARFKNLSFDPKNKYSIPYFWGTVGIVYNDQLVKTPPKHWDD
                     LWRPEFRNKIMLVDSAREVIGVGLNSLGYGLNTKNISELKAASKKLDALTPNVKAIVA
                     DEMKGYMIQGDAAIGVTFSGEAREMLDGNKHLHYVVPSEGSNLWFDNIVIPKTVKHRK
                     EAYAFINFMMEPKNAAQNAEYIGYATPNLKAKALLPADIKNDKAFYPPDKTIDHLEVY
                     NNLGQKWLGIYNDLYLQFKMYRK"
     gene            complement(3152..3928)
                     /locus_tag="VBC28_001569"
     CDS             complement(3152..3928)
                     /locus_tag="VBC28_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6815541.1"
                     /translation="MKKFANIYLALVFIILYIPIIYLIFYSFNKGGDMNSFTGFTFSH
                     YGELFQDSRLMLILVQTFFLAFLSALLATIIGTFGAIWIYQVRRRHQTSILSLNNILL
                     VAPDVMIGASFLLVFTVIGLQLGFTSVLLSHVAFSIPIVVLMVLPRLKEMNDDMINAS
                     YDLGASTWQMLKEVMLPYLSSGIISGFFMAFTYSLDDFAVTFFVTGNGFSTLSVEIYS
                     RARRGISLEINALSTIVFLFSILLVIGYYYISKEKGEKNA"
     gene            complement(3925..4719)
                     /locus_tag="VBC28_001570"
     CDS             complement(3925..4719)
                     /locus_tag="VBC28_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6815542.1"
                     /translation="MKKTSSLFSIPYMAWLFLFVLAPVALIAWNSFFDINGHFTLANY
                     QTFFSSGTYLKMSFNSVLYAGIVSFITLLISYPAAYLLTKLKHKQLWLMLVILPTWIN
                     LLLKAYAFMGIFGQQGGINAFLTFIGIGPKQILFTDFSFLFVAAYIELPFMLLPIFNA
                     LDDIDQNLIYASDDLGANAWQTFQKVIFPLSLNGVRAGVQSVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLITQNKGMGSTIGVILILVMVAIMWLTKERKA"
     gene            complement(4703..5857)
                     /locus_tag="VBC28_001571"
     CDS             complement(4703..5857)
                     /locus_tag="VBC28_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6815543.1"
                     /translation="MTNPIIAFKNVSKVFEDSNTVVLKDINFELEEGKFYTLLGASGS
                     GKSTILNIIAGLLEASTGDIYLDGKRINDVPTNKRDVHTVFQNYALFPHMTVFENVAF
                     PLKLKKMDKKEIQKRVQETLKMVRLEGFEKRAIQKLSGGQRQRVAIARAIINQPKVVL
                     LDEPLSALDLKLRTEMQYELRELQQRLGITFVFVTHDQEEALAMSDWIFVMNEGEIVQ
                     SGTPVDIYDEPINHFVATFIGESNILSGKMIEDYLVEFNGKRFEAVDGGMRPNESVQV
                     VIRPEDLQITLPDEGKLQVKVDTQLFRGVHYEIIAYDDLGNEWMIHSTRKAIEGEVIG
                     LDFTPEDIHIMRLNETEEEFDARIEEYVDTDDHEDGLINAIEEERNEENL"
     gene            complement(5906..6808)
                     /gene="murB"
                     /locus_tag="VBC28_001572"
     CDS             complement(5906..6808)
                     /gene="murB"
                     /locus_tag="VBC28_001572"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEO6815544.1"
                     /translation="MIKTIQKELEGLDIRFDEPLKKYTYTKVGGPADYLAFPRNRLEL
                     SRIVKFANSQNIPWMVLGNASNIIVRDGGIRGFVIMFDKLSTVTVNGYVIEAEAGANL
                     IETTRIARYHSLTGFEFACGIPGSVGGAVFMNAGAYGGEIAHILLSAQVLTPQGELKT
                     IEARNMQFGYRHSVIQESGDIVISAKFALKPGDHLMITQEMDRLTYLRELKQPLEYPS
                     CGSVFKRPPGHFAGQLISEAHLKGQRIGGVEVSQKHAGFMVNIAEGSAQDYENLIEHV
                     INTVESTSGVHLEPEVRIIGESLL"
     gene            complement(6952..7440)
                     /gene="folK"
                     /locus_tag="VBC28_001573"
     CDS             complement(6952..7440)
                     /gene="folK"
                     /locus_tag="VBC28_001573"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEO6815545.1"
                     /translation="MTTVYLSLGSNIGDRETFLKQALFSIDHLQKTKVAQISAIYETA
                     AWGNTNQEDFFNICCQVETDLAPFELLDYCQEIEKRLKRVRHEHWGPRTIDIDILLFG
                     NQVINQEDLVVPHPYMTKRAFVLVPLLEIAPQLSLPNGSKLEDYLEKLNLGEVHYLKP
                     VE"
     gene            complement(7437..7799)
                     /gene="folB"
                     /locus_tag="VBC28_001574"
     CDS             complement(7437..7799)
                     /gene="folB"
                     /locus_tag="VBC28_001574"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEO6815546.1"
                     /translation="MDKIYLNKCRFYGYHGAFSEEQTLGQVFQVDAVLSLDLAKASQT
                     DDLIDTVHYGEVFDCIKNHVENEQYQLIEKLAGVIVEDIFLQFHPVQAITLKITKDNP
                     PINGHYESVGIELERKRV"
     gene            complement(7801..8604)
                     /gene="folP"
                     /locus_tag="VBC28_001575"
     CDS             complement(7801..8604)
                     /gene="folP"
                     /locus_tag="VBC28_001575"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEO6815547.1"
                     /translation="MKIGQYDITGKACIMGILNVTPDSFSDGGSYTTIDSALNQVGEM
                     LEQGVAIVDIGGESTRPGAVFVTAEEEIKRVVPMIKAIREVYPDLLLSIDTYKTEVAQ
                     AALDAGVHILNDVWSGLYDGKMLSLAAERNVPIILMHNQEEAVYQDIKKEVCEFLLER
                     AERALEAGVSKDNIWIDPGFGFAKTEEQNLELLKGLEQVCDLGYPVLFGISRKRTVNY
                     LLGGNREVTERDMGTAALSAWAIAKGCQIVRVHNVEVNKDIVTVISQLV"
     gene            complement(8608..9171)
                     /gene="folE"
                     /locus_tag="VBC28_001576"
     CDS             complement(8608..9171)
                     /gene="folE"
                     /locus_tag="VBC28_001576"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001133588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEO6815548.1"
                     /translation="MTNQEKMEKAIYQFLEALGENPDREGLKDTPKRVAKMYIEMFSG
                     LNQDPKEQFTAVFSENHEEVVIVKDIPFYSMCEHHLVPFYGKAHIAYLPNDGRVTGLS
                     KLARAFEVASKRPQLQERLTAQVAQALEDALAPKGIFVMIEAEHMCMTMRGIKKPGSK
                     TITTVARGLYKDDRYERQEILSLIQKG"
     gene            complement(9190..10452)
                     /locus_tag="VBC28_001577"
     CDS             complement(9190..10452)
                     /locus_tag="VBC28_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEO6815549.1"
                     /translation="MTYQEALEWIHSKLAFGIKPGLERMRWMLEQLGNPQNNLSAIHV
                     VGTNGKGSTTSYLQHIFTNSGYQVGTFTSPYIVDFRERISIDGQMIPESDFIKLVETV
                     RPVVERLHLETNLEPATEFEVITVLMFYYFGNSCPVDIVIIEAGMGGYYDSTNMFKAL
                     AVTCPSIGLDHQEVLGRTYVDIAEQKVGVLKKGVPFVYANDRQDVEEVFQIKAKETHS
                     QTYRLHNDFYIKEEENYFNYIGPQANIDHIQLQMPGHHQVSNASIAITTSLLLRDKYP
                     KLTLQTIKDGLEMTKWVGRTELIFPNVMIDGAHNNESVDALVQVIKKYQQKNVHILFA
                     AINTKPIESMLESLSSIAPVSVTSFDYPKSINLDKYPKAYTRVSDWKKWLHDINLTSD
                     KDFYVITGSLYFISQVRQELLLIKTTTT"
     gene            complement(10454..>10680)
                     /locus_tag="VBC28_001578"
     CDS             complement(10454..>10680)
                     /locus_tag="VBC28_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HEO6815550.1"
                     /translation="TAVPLLLFAEAIKRAPLNIIGFIQYINPTIQLLLALFIFKETIV
                     SGEVIGFIFIWLAILVFSIGQVHTMLKKGK"
BASE COUNT         3584 a         2128 c         1596 g         3372 t
ORIGIN      
        1 tgatgatacc tacagaaaaa acgagagaat tcgtgatctg attgattata ttaataataa
       61 ttaccagcaa aatcttacca ttgaattttt agctgattat atggggtata gtaagacaca
      121 ttttatgacc gtatttaaac agcatactgg aacatcctgt acagaattta tcatacaagt
      181 tcgtcttaat aaagctagtg agcatcttat taactccact acggccatta ttgatattgc
      241 caacagcgtt ggttttaata atctatcaaa ttttaatcgc caatttaaac gctattatca
      301 tacaactcct cgccagtacc gcaaacaatt taccaacaaa aaagacggta gtacgcttgt
      361 ctatagaaat ataaatacaa aaaaatagaa actagttaaa tccatgacta gtttctattt
      421 tttattattt aagcgttaat tagtttacat cagctgctca cgtacttctt taatttccga
      481 ttcattcaca actaaaaaaa tggtgtcacc agcattcaag atagtatttc ctgatactac
      541 agccgatgtc ttatgatgaa tttgagtcgt tattagcaca ttttctggta attctaaatc
      601 tcggacatac tttcctgata ttttatctga aacagttaat tcaatcaatg taggtgtcat
      661 tactttatct tttggattga aggtcatttt agccaacatc gcttcataaa taggttctcc
      721 ctttaataaa tccatgacaa tataagatac cattgttaca attccaatag ccattaattg
      781 ttttaaatcg ccaaccattt ctgtaaccaa aatcatagct gttagtggtg cttttgagat
      841 tgcaccaaaa tagcccgcca ttcctagaac aacaaataaa gatatctgct gatgagatat
      901 tataccaaag tactgcatac ctactgcaaa aaaagcacct gctaaagatc ctaaagctag
      961 aattggtaaa aaaattcctc ctggcaagcc actactgtaa ctcaacatac tccataaaaa
     1021 acgaatgagg aaaaataatc ctaacatcat cagactaaga ttactcctag gcagtgatac
     1081 aattaagcca tttccacccc ccaataattg tgggaaatag taaccaatag gaagaataaa
     1141 aattaccgct aatataccat ataggtgaga tggtagatga aatagtttac ctaaataatc
     1201 atagatcaca tgaaatctca aaattaccca ctcatagata aagcctaaga tacccaaaaa
     1261 gagacccatg agtaaaaaaa tccaataaaa gttgagattt aaagatggta attctgatgg
     1321 taaagctaaa actggggtta aaccaaagat attaagtgag acaaagtttg ccactaaact
     1381 tgctaccaaa gctgttatcc aaactaagcg agaaaaatga tggtaaattt cttcaactac
     1441 aaataaaagg cctgcaatag gagcattgaa ggctgctgaa agacctgctg ctgcaccact
     1501 agcaattaaa acacgttttt ccattcgact agcattgaga tactgtgcaa ttcccttacc
     1561 tgtcatagct cctaactgaa tactgggacc ttcgcgacca agcataagtc ccgaagaaat
     1621 agctaaaata cccgcaatga atttcttcca tacaatacta aaccagtctg gcgatagcat
     1681 tcctttaagt tcaccttcaa catgcgggat acctgaacct ttaatatcag gatcagattt
     1741 gataaaaaaa cctattatca ctgcagcgat aatactagta accgtaatgc tcaataaaat
     1801 aattggttga taatgggcac ttttataaag ttccataaca actaaaaaaa ttttttcaat
     1861 cgctaatcga aatatgctga caacaaatcc tgatattaag cctactacta ttcctctcca
     1921 aacaaatccc agaattgatt caagtgaaaa ctgaaattca tttttatgat tttccatatt
     1981 ttcctccttt tcttacaatt attttaacaa aaatatcaca aactttctag gaactcttgg
     2041 taactaaaaa tacctctcat cagagaagta cttttagtta atagattact tacgatacat
     2101 tttaaattgt aagtacaaat cattataaat accaagccat ttttgaccca agttattata
     2161 aacttctaaa tgatcaattg ttttatcagg aggataaaat gctttatcat ttttgatatc
     2221 tgctggtaac agcgcttttg ctttgagatt aggggtcgca taaccaatat attctgcatt
     2281 ttgtgccgca tttttaggtt ccatcatgaa attaataaag gcataagctt ctttgcgatg
     2341 ctttactgtt ttggggatta caatattatc aaaccaaagg ttcgatcctt cactaggaac
     2401 aacgtaatgc aaatgcttgt tcccatcaag catttctcgt gcttctccag agaatgtaac
     2461 accaatcgct gcatctccct gaatcatata gcctttcatc tcatcagcaa caatagcctt
     2521 aacgtttggt gttagagcat ctaacttttt agaagcggct tttaattctg atatgttctt
     2581 tgtattcaat ccatatccca aggaatttaa accaactcca ataacctcac gtgcactatc
     2641 aactagcatg attttatttc gaaactctgg acgccataaa tcatcccaat gtttaggagg
     2701 agttttaact aattgatcat tataaacaat cccaacagtt ccccaaaaat aaggaatcga
     2761 atatttattt ttaggatcga aactcaaatt tttaaaccta gctccaatag catcccaatt
     2821 agctatcttt gaatgatcaa gtttaaccag aaggttttct tttatcattt tgtcaatcat
     2881 ataatcacta ggtacagcaa tatcatatgt cgtcccaccc tgcttaattt tggtgtgcat
     2941 agcctcatta gagtcaaacg tttcgtattg tacttcaatt ccagtttctt ttgtaaattt
     3001 ttttaataag gctggatcaa tatagtctcc ccagttataa ataactaact tatctgagtt
     3061 aggtgttgaa ctactcttct tttctaagta agtggttaaa ccaaagagga tcaggataac
     3121 aagaataata ccgcctaaaa aggaataaac tctacgcatt cttttctccc ttctctttcg
     3181 aaatatagta ataaccaatt accaataaaa tactaaataa aaagacaata gttgacaagg
     3241 cattaatttc caatgatata ccgcgacgtg cgcgtgaata gatttctact gaaagcgttg
     3301 aaaagccatt acctgtaaca aagaaagtaa ccgcaaaatc atctagcgag taagtgaaag
     3361 ccataaagaa acctgaaata atacccgacg acaaataggg taacataact tctttaagca
     3421 tttgccaagt tgaagctcct aaatcatagg aagcattaat catatcatca ttcatttctt
     3481 ttaaacgagg taagaccatt aaaacaacaa taggaataga gaatgcaaca tggctcaata
     3541 atacagaagt gaaacctaac tgtaaaccaa taacagtaaa tactaagaga aaacttgcac
     3601 caatcataac atctggtgct actaaaagga tattattaag cgataaaata gaggtttgat
     3661 gtctacgtct tacctgataa atccaaatag ctccaaaggt accaataatt gtagctaata
     3721 aggcgctaag aaaagccaaa aagaatgttt gcactaaaat tagcattaag cggctatcct
     3781 gaaacagttc tccatagtga ctaaaggtga atccagtaaa agaattcata tccccacctt
     3841 tattgaaaga ataaaaaatc aaataaataa taggaatata aaggataata aaaacaagtg
     3901 ctaaatagat atttgcgaat tttttcatgc tttcctctcc tttgttaacc acataatagc
     3961 aaccatcact aaaattaaaa taacccctat ggttgagccc atacctttat tttgtgtgat
     4021 taaaaaatgt tgttctatgg ctgtccctaa agtaataact cgattaccac caataagacg
     4081 cgtaagcata aagagactta aactaggaat aaagacagat tgtacacctg ctcttacacc
     4141 attcagagat agaggaaata taaccttctg aaaagtttgc catgcatttg cacccaagtc
     4201 atcacttgca taaatgagat tttgatcaat atcatccaag gcattaaaaa tgggtaataa
     4261 cataaacggt aattcaatgt aagctgctac aaaaagaaat gaaaaatctg taaatagaat
     4321 ctgttttggg ccgataccta taaaggttaa gaaggcgtta attccccctt gttgaccaaa
     4381 tatccccata aaagcatagg cttttaacaa aagattaatc catgttggca aaataactag
     4441 cattagccat aattgcttgt gttttaattt tgtaagcaaa taagcagctg gatagctaat
     4501 caaaagcgtt atgaaactaa cgatgcctgc atataggacc gagttaaagc tcattttaag
     4561 ataggttcct gagctaaaaa acgtctggta attagctaaa gtaaagtgcc cattaatatc
     4621 aaagaacgag ttccaagcaa tcaaagcaac aggtgctaat acaaataaga ataaccaagc
     4681 catataagga attgaaaata gactagaggt tttcttcatt tctctcctcc tcgatagcat
     4741 tgattaaacc atcttcatga tcgtcagtgt ctacatattc ttcaatgcgc gcatcaaatt
     4801 cttcttctgt ttcattaaga cgcataatat gtatatcttc tggagtgaaa tctaacccaa
     4861 taacttcacc ctcaatagct ttacgagttg aatgaatcat ccattcatta cctaaatcat
     4921 cataagcaat aatttcatag tggacacctc gaaaaagttg tgtgtccact ttaacttgaa
     4981 gttttccttc atcgggtagg gtaatttgaa gatcttctgg acgaataact acttggactg
     5041 attcattagg gcgcataccg ccatcaacag cctcaaagcg cttaccgtta aattcaacca
     5101 aataatcctc aatcatcttg ccagataaga tatttgattc acctataaaa gttgctacaa
     5161 aatgattgat aggttcatcg tagatatcta ctggtgttcc tgattgaaca atttcacctt
     5221 cattcatgac gaaaatccaa tcactcattg ccaacgcttc ttcttgatca tgtgtgacaa
     5281 aaacaaaggt aattcctaaa cgttgttgca gttcgcgcaa ttcatattgc atctctgtac
     5341 gcaatttcaa atccaatgct gataagggtt catctagtaa gactacttta ggttgattga
     5401 tgatagcacg cgctatcgca acacgctggc gttgtcctcc tgataatttt tgaatagctc
     5461 gcttttcaaa cccttctaaa cgaaccattt ttagagtttc ttgaacacgt ttttgaattt
     5521 ctttcttatc catttttttc aactttaatg gaaaagcaac gttttcaaaa accgtcatat
     5581 gtggaaacaa agcatagttt tggaaaacgg tatgaacatc ccgtttatta gtgggaacat
     5641 cattgatgcg tttcccatct aagtaaatat cgcctgtact cgcctctagc aaacctgcaa
     5701 taatatttaa gatagttgat ttaccacttc ctgaggcccc taataaggtg taaaatttac
     5761 cttcttcaag ttcaaaatta atatctttta agacaaccgt gttgctgtct tcgaatacct
     5821 ttgatacatt tttaaatgca atgattgggt tagtcaattc tcataaatcc tccatttttc
     5881 tgactaaagt aagtactaaa ctactttata atagcgactc accgataata cgaacttctg
     5941 gttccaaatg gacacctgaa gttgattcaa cggtgttaat aacatgttca ataagatttt
     6001 cataatcttg agctgaacct tctgcaatgt taaccataaa accagcatgt ttttgactaa
     6061 cctctactcc accaatacgt tgacctttaa gatgtgcttc gctaatgagt tgccctgcaa
     6121 aatgtcctgg aggacgtttg aacacagaac cacaagaagg gtattctaaa ggttgtttca
     6181 gctcacggag ataggttaaa cgatccattt cctgagtaat catgagatga tctcctggtt
     6241 ttaaggcaaa cttagctgat ataacaatat ctccactctc ttgaatgaca gaatgacgat
     6301 aaccaaattg catattccta gcttcaatcg ttttgagttc accctgtggt gtcaaaactt
     6361 gtgcagacaa taaaatatga gcaatttcac caccgtaagc gccagcattc ataaagactg
     6421 ctccacctac acttccagga attccgcaag caaactcaaa gccagttaaa ctatgataac
     6481 gtgcaatacg agttgtttca atgagattag ctcctgcttc tgcttcaata acataaccgt
     6541 tgactgtaac agttgacagt ttatcaaaca tgataacaaa accacggata ccaccatctc
     6601 taacgataat attactcgca tttcctaaaa ccatccaagg aatattttga ctgttagcaa
     6661 acttaacaat acgcgatagt tcaaggcgat tccttgggaa tgcaagataa tcagcaggtc
     6721 ctcctacttt agtataagta tatttcttta aaggctcatc aaagcgaata tcgagacctt
     6781 ctaattcttt ttgtattgtt ttaatcataa cctttatctc aattctgttt tattgtgttt
     6841 tatagacaca aaaaatcggc ataaaaccga tttctattat accaaaaata taggttgttg
     6901 acgatacatt atcacaattt ctgtgtattt taattttaaa atcctaactg actattcgac
     6961 tggttttaaa taatgaactt ctcctaaatt caatttttcg agatagtctt ctagcttaga
     7021 accattaggt aaacttagtt gaggtgctat ttcgagtaac ggaactagaa caaatgctcg
     7081 cttcgtcata tacggatggg gtactactaa atcttcttgg ttaatcactt gattaccaaa
     7141 aagtaagata tcaatatcta ttgtccttgg accccagtgc tcatgtctca cgcgctttaa
     7201 gcgtttttca atttcttgac aataatccaa taattcaaaa ggtgctaagt ctgtctctac
     7261 ctgacaacat atattaaaaa aatcttcttg gttagtattc ccccaagcag ctgtttcata
     7321 aatagcggaa atttgcgcga cttttgtctt ttgcaaatga tcaattgaaa agagagcctg
     7381 cttcagaaaa gtttctctat caccaatatt actacctaaa ctcaagtaaa cggttgtcat
     7441 actcgcttgc gctccaattc aattccaact gattcatagt gcccattaat tggtggattg
     7501 tcttttgtaa tctttaatgt aatagcttgt actggatgaa actgtaagaa aatatcttca
     7561 acaataacac ctgctaattt ctctattaat tgatactgtt cgttttcaac atgattttta
     7621 atacagtcaa aaacttcacc ataatgaacc gtatcaatta aatcatcagt ttgtgatgct
     7681 tttgctaaat caagtgatag aacagcatca acttgaaaaa cttgccccaa tgtttgttct
     7741 tccgaaaaag caccatgata accataaaac cgacatttat ttaaataaat cttatccata
     7801 ttacaccaac tgactaatta ctgtcacaat gtccttattt acttcaacat tgtgaacacg
     7861 tacaatctga cacccctttg ctattgccca tgccgataaa gcagcagtcc ccatatcacg
     7921 ttcagttact tccctattac cacctagaag ataattaaca gtacgttttc gagagatccc
     7981 aaataacaca ggataaccta aatcacacac ttgttccaaa ccttttaata gctctaaatt
     8041 ttgctcctct gttttagcaa aaccaaagcc aggatctatc caaatattat cctttgaaac
     8101 acctgcttct aaagcacgct ctgctctttc aagtaagaat tcacaaactt cttttttgat
     8161 atcttgataa acagcttctt cctggttatg catcaaaata attggaacat ttcgttctgc
     8221 tgctaaagat agcattttac catcatataa acctgaccaa acgtcattta aaatatgtac
     8281 acctgcatca agggctgctt gagctacctc tgttttataa gtatctattg ataacaaaag
     8341 atcagggtag acctctctaa tagctttaat cattggaact acacgtttaa tttcttcttc
     8401 agcagtcaca aatactgcac ctggccgcgt tgattctccc ccaatatcta caattgccac
     8461 cccttgttca agcatctcac caacttgatt taaagctgaa tcaattgtgg tataagaacc
     8521 accatctgag aaggaatcag gcgttacatt taaaatcccc atgatacaag ctttacctgt
     8581 aatatcatat tgtcctattt tcatctttta tcctttttgt attagcgata gaatttcttg
     8641 tctttcatat cgatcgtcct tgtataaacc acgagctacc gttgtaatcg tcttacttcc
     8701 tggtttttta atgccacgca ttgtcatgca catatgctct gcttctatca taacaaaaat
     8761 acctttaggc gctaaagcat cctctaaagc ttgtgcaact tgtgctgtta aacgttcctg
     8821 aagttgagga cgcttactcg ccacttcaaa tgcacgtgca agcttactaa gtccagtaac
     8881 acgtccatcg tttggtaaat aagcaatatg agctttacca taaaaaggaa ccaaatgatg
     8941 ttcacacatg gaatagaatg gaatatcctt gacaatcaca acctcttcat gattttcaga
     9001 aaagactgca gtgaattgct cctttggatc ttgattaagt cctgagaaca tttctatata
     9061 catttttgcc acacgcttcg gtgtatcttt caatccttct cgatcaggat tttctcctaa
     9121 agcctctaaa aattgataaa tagctttttc cattttttct tgattggtca ttaatttatc
     9181 ctatcctttt taagttgttg tagttttgat taatagtagt tcctgacgaa cttgagaaat
     9241 aaaataaagg gagccagtaa tcacatagaa atctttatct gatgtcagat ttatatcatg
     9301 tagccatttc ttccaatcac taactctagt gtacgcttta ggatatttgt ctaagtttat
     9361 tgatttaggg taatcaaagc tagtaaccga aaccggagcg atactagaca atgattctaa
     9421 catactctct atcggtttag tattaattgc agcaaataga atatgcacgt ttttctgctg
     9481 atattttttt ataacttgaa cgagagcatc gacactttca ttattatgag cgccatcaat
     9541 cattacattt ggaaagatta attctgtacg gcctacccat tttgtcattt caagcccatc
     9601 tttaatggtc tgtagggtta actttggata tttgtctctt aataataaac tggtagttat
     9661 agctatgcta gcattggaaa cttggtgatg tcccggcatt tgcaactgaa tatgatcaat
     9721 atttgcttga ggacctatat agttaaaata attttcctct tcttttatat aaaagtcatt
     9781 gtgtaaacga taagtctggc tgtgggtttc cttggctttt atttgaaaga cttcttcaac
     9841 atcctgacga tcattagcat atacgaatgg aactcctttt tttaaaacac ctactttttg
     9901 ttcagctata tctacataag ttcgtcctaa aacctcctga tgatctaatc caattgacgg
     9961 acaagtaacc gctaaagcct tgaacatgtt ggtagaatca taataaccgc ccattccagc
    10021 ctcaataatg acgatatcaa ctggacagct attgccaaaa taatagaaca tcaaaactgt
    10081 tataacttca aattctgtag ctggttctag gttggtctca aggtgcaacc gttcaacaac
    10141 tggacgaaca gtttcaacaa gtttgataaa atcagactct ggaatcattt gaccatcaat
    10201 gctaatacgc tctctaaagt caacaatata tggtgatgta aatgttccaa cttgataacc
    10261 agaatttgta aaaatatgtt gtaaataact agttgttgat cctttaccat tcgttccaac
    10321 aacatgaatg gcagataggt tgttttgagg gtttcctaac tgctctagca tccatctcat
    10381 acgttccaaa cctggtttaa taccaaaagc taatttgcta tgaatccatt ctagcgcttc
    10441 ttgataagtc atcttattta cctttcttta acatcgtatg tacttgtcca atactaaaaa
    10501 ctaaaatagc aagccagata aaaataaaac ctattacctc cccactaacg atagtttctt
    10561 taaaaataaa caatgctagc aataattgga tggttggatt gatgtattgg ataaaaccga
    10621 taatatttag aggagccctc ttaatggctt ctgcaaataa taaaagagga actgctgtaa
//
LOCUS       DATRMA010000065        10426 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.065, whole genome shotgun sequence.
ACCESSION   DATRMA010000065 DATRMA010000000
VERSION     DATRMA010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10426
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.065"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            124..609
                     /locus_tag="VBC28_001579"
     CDS             124..609
                     /locus_tag="VBC28_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6815551.1"
                     /translation="MRETETHDHQALIQKLLVSIHYLTLFRDEIILVEKTPSLLGKHF
                     SIAIVQNELGEILSKIEALSKQKKLIRSIYWYDESSFKVMNKALAIVEEWIKGLDNLL
                     EFCQSQTVFQAILGDERAHVFGILIDVYTSLNIINTSLKEENSRPVSLSDLALGLKDE
                     D"
     gene            708..1361
                     /locus_tag="VBC28_001580"
     CDS             708..1361
                     /locus_tag="VBC28_001580"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEO6815552.1"
                     /translation="MKHSSWHDLIKRELPNQYYNKINTFMDAVYESGIVYPPRGKVFN
                     AIQITPLENVKVVIIGQDPYHGPQQAQGLSFSVPDNLPAPPSLQNILKELAEDIGSRS
                     HHDLTSWAQQGVLLLNACLTVPEHQANGHAGLIWEPFTDAVIKVVNQKETPVVFILWG
                     GYARKKKSLIDNPIHHIIESPHPSPLSAYRGFFGSRPFSRTNHFLEEEGINEIDWLN"
     gene            complement(1427..2065)
                     /gene="plsY"
                     /locus_tag="VBC28_001581"
     CDS             complement(1427..2065)
                     /gene="plsY"
                     /locus_tag="VBC28_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEO6815553.1"
                     /translation="MSIIIMIIIAYLLGSIQTGLWIGKYFYQVNLRQHGSGNTGTTNT
                     FRILGVKAGIVTLTIDILKGTLATLIPIMLGVTTVSPFFIGFFAIIGHTFPIFAQFKG
                     GKAVATSAGVLLGFAPSFFLYLLVIFLLTLYLFSMISLSSITVAVVGILSVLIFPLVG
                     FILTDYDWIFTTVVILMALTIIIRHQDNIKRIRKRQENLVPFGLNLSKQRNK"
     gene            2179..4128
                     /gene="parE"
                     /locus_tag="VBC28_001582"
     CDS             2179..4128
                     /gene="parE"
                     /locus_tag="VBC28_001582"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEO6815554.1"
                     /translation="MAKQDITVTNYGDDAIQVLEGLDAVRKRPGMYIGSTDGTGLHHL
                     VWEIVDNAVDEALSGFGNRIDVIINKDGSITVTDHGRGMPTGMHAMGKPTVEVIFTVL
                     HAGGKFGQGGYKTSGGLHGVGSSVVNALSSWLEVEIIRDGAIYRQRFENGGKPVTTLK
                     KIGTAPKSKSGTSVSFMPDQSVFSTIDFKFNTIAERLKESAFLLKNVTLTLTDNRSEE
                     AEHLEFHYENGVQDFVEYLNEDKETLTPIMFFEGEEQEFHIEVALQYNDGFSDNILSF
                     VNNVRTKDGGTHETGLKSAITKSMNDYARKTGLLKEKDKNLEGSDYREGLSAILSILV
                     PEEHLQFEGQTKDKLGSPLARPIVDGIVSEKLTYFLMENGDLASNLIRKAIKARDARE
                     AARKARDESRNGKKSKKDKGLLSGKLTPAQSKNAKKNELYLVEGDSAGGSAKQGRDRK
                     FQAILPLRGKVLNTAKAKMADIIKNEEINTMIHTIGAGVGPDFNIDDINYDKVIIMTD
                     ADTDGAHIQTLLLTFFYRYMRPLVEEGHVYIALPPLYKMSKGKGKKEIVEYAWTDIEL
                     EELRQKFGKGSLLQRYKGLGEMNADQLWETTMNPETRTLIRVTIEDLARAERRVNVLM
                     GDKVPPRRQWIEDNVKFTLEENTVF"
     gene            4262..6721
                     /gene="parC"
                     /locus_tag="VBC28_001583"
     CDS             4262..6721
                     /gene="parC"
                     /locus_tag="VBC28_001583"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEO6815555.1"
                     /translation="MSNIQNMSLEDIMGERFGRYSKYIIQERALPDIRDGLKPVQRRI
                     LYSMNKDGNTFEKGFRKSAKSVGNVMGNFHPHGDFSIYDAMVRMSQDWKNRETLIEMH
                     GNNGSMDGDPAAAMRYTEARLSEIAGYLLQDIDKNTVPFAWNFDDTEKEPTVLPAAFP
                     NLLVNGATGISAGYATDIPPHNLAEVIDAVVYMIDHPKAKLDKLMEFLPGPDFPTGAI
                     IQGKDEIRKAYETGKGRVAVRSRTAIETLKGGKKQIIVTEIPYEVNKSVLVKRIDDVR
                     VNNKVPGIAEVRDESDRDGLRIAIELKKEADETIVLNYLFKYTDLQVNYNFNMVAIDD
                     YTPKQVGLSRILTSYIAHRREIIIARSKFDKEKAEKRLHIVEGLIRVLSILDEVIALI
                     RASENKADAKENLKVSYEFSEAQAEAIVTLQLYRLTNTDIVTLREEEEELRQQITMLK
                     AIISDERTMYNVMKRELREVKKKFANTRRSELQELAETIEIDTASLIIEEDTYVSVTR
                     GGYVKRTSPRSFNASTVDELGKREDDELIFVSNAKTTQHLLMFTNLGNLAYRPVHELA
                     DIRWKDVGEHLSQNLVNFASNEEIIYAELVDDFTKETYFAVTRLGQIKRFERQEISPW
                     RTYKSKTAKYAKLKNVDDYVVTVAPIQLEDVILVTYNGYALRFSINDVPVVGSKAAGV
                     KAMNLKDSDHIVSAFIANTTSLYLLTHRGSLKRMAIDVIPTTSRANRGLQVLRELKSK
                     PHRVFKAGPVYLEDSSFEFDLFSSVSNHEGDTFVLEIMSKTGKVYDVDLSQWSFSERT
                     SNGSFVSDKISDEEVFSVKIK"
     gene            6834..7856
                     /locus_tag="VBC28_001584"
     CDS             6834..7856
                     /locus_tag="VBC28_001584"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012961903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HEO6815556.1"
                     /translation="MTVNLDWDNLGFAYRKLPFRYISHFKDGKWDDGKLTDDATLHIS
                     ESSPALHYGQQAFEGLKAYRTKDGSIQLFRPDQNAERLQRTADRLLMPHVPTDKFIAA
                     VKSVVRANEEFVPPYGTGATLYIRPLLIGVGDIIGVKPAEEYIFTVFAMPVGSYFKGG
                     LTPTNFIVSKEYDRAAPNGTGAAKVGGNYAASLLPGKYAHEKQFSDVIYLDPATHTKI
                     EEVGAANFFGITKDNQFITPLSPSILPSITKYSLLYLAKERFGMEAIEGDVFVDELDK
                     FTEAGACGTAAVISPIGGIQNGDDFHVFYSETEVGPATRKLYDELVGIQFGDVEAPEG
                     WIYKVD"
     gene            7939..8169
                     /locus_tag="VBC28_001585"
     CDS             7939..8169
                     /locus_tag="VBC28_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001870505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HEO6815557.1"
                     /translation="MSKKDKKIEIQISDAQVTVNGTKVDGYQLVIGKKIVGQIAELDS
                     KFAVIKGEQVSGFYKNLDQAMGNIIEEYNLNR"
     gene            8230..8310
                     /locus_tag="VBC28_001586"
     tRNA            8230..8310
                     /locus_tag="VBC28_001586"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8264..8266,aa:Tyr,seq:gta)
     gene            8316..8387
                     /locus_tag="VBC28_001587"
     tRNA            8316..8387
                     /locus_tag="VBC28_001587"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8348..8350,aa:Gln,seq:ttg)
     gene            8513..9715
                     /gene="rpsA"
                     /locus_tag="VBC28_001588"
     CDS             8513..9715
                     /gene="rpsA"
                     /locus_tag="VBC28_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002997242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEO6815558.1"
                     /translation="MNEFEDLLNSVSEVNPGDVVTAEVLTVDNGQANVVIDGTGVEGV
                     LTLRELTNDRDADINDFVKAGDTVEVLVLRQVVGKDTDTVTFLVSKKRLEARKAWDKL
                     VGREGEVVTVKGTRAVKGGLSVEFEGLRGFIPASMIDTRFVRNTEKFVGQEFDAKIKE
                     VDAAENRFILSRREVVEESAAAARKEVFSNIEVGSVVTGKVARLTSFGAFIDLGGVDG
                     LVHVTELSHERNVSPKSVVTVGEEVEVKVLSIDEEAGRVSLSLKATTPGPWDGVEQKL
                     AAGDVIEGKVKRLTDFGAFVEVLPGIDGLVHISQISHKRVENPKDVLSAGQEVTVKVL
                     EVNSDAERVSLSMKALEERPAQAEGEKEEKRQSRPRRPRRQEKRDYELPETQTGFSMA
                     DLFGDIEL"
     gene            9788..9859
                     /locus_tag="VBC28_001589"
     tRNA            9788..9859
                     /locus_tag="VBC28_001589"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9821..9823,aa:Arg,seq:ccg)
     gene            complement(9895..>10426)
                     /locus_tag="VBC28_001590"
     CDS             complement(9895..>10426)
                     /locus_tag="VBC28_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEO6815559.1"
                     /translation="EVKYKSHFCIYLIATTGLRFSEAMGLTWSDIDRDELLLHVNKTY
                     KVFGSDKGFQPTKNKHSIRYVPINNTTIKLLDQYRLLFKTETRIFEGISNTAVNKTLK
                     KLVGRNIHIHSLRHTYVSHLISEGIDLFAISKIVGHKDLNITLSTYAHLLEDTKNKNN
                     EIIRELFGANLGHKLI"
BASE COUNT         3443 a         1652 c         2096 g         3235 t
ORIGIN      
        1 aaccttataa tatttatttt ataaaattat tcatcttatt tttgcgattt caatagtgat
       61 atggtatatt taaagtaagc tacgtatagt tttaccagtc agaaatagta aggatggttg
      121 agtatgagag aaactgaaac acacgatcac caagcgctaa ttcaaaaatt gttagtcagc
      181 attcattatt taaccttgtt tcgtgatgaa attatattag tagaaaaaac cccatcttta
      241 ctggggaaac atttttctat tgctattgta cagaatgaac taggtgagat actttctaaa
      301 atagaagctt tgagtaagca aaaaaaactg attcgttcta tttattggta tgatgaatca
      361 tcatttaaag tcatgaataa ggctctggct attgtagaag aatggattaa gggcttagat
      421 aatcttttag aattctgtca atcacagacg gtttttcaag caattttagg tgatgaacgt
      481 gcacatgtat ttggaatcct tattgatgtt tatacttctt taaacattat aaatacttct
      541 ttgaaagaag aaaattctag accagtgtcc ttatctgatt tggctctagg tcttaaggac
      601 gaagattaat aaaaaaacgt tactgctttg aatgaataaa gcagtaacgt tttttataaa
      661 atattgctat actagtaata cagatatcta aaagtaagga gttataaatg aaacattcta
      721 gttggcatga tttaataaaa agagaactac ccaatcaata ctacaataaa attaatacat
      781 ttatggatgc agtttacgaa agcggaatcg tttatcctcc aagaggtaag gtgttcaatg
      841 ccatacagat aacaccacta gaaaacgtaa aagtagttat tattggccaa gatccttatc
      901 atggccctca acaagctcaa ggactctcat tttctgttcc agacaattta ccagctcctc
      961 cttcacttca aaatattcta aaagaattag cagaagatat cgggtcacgt tcacatcatg
     1021 acttaacatc atgggcgcaa caaggagtgc tgctattgaa tgcttgttta acagttccag
     1081 aacaccaagc taatggtcat gctgggctta tatgggaacc ttttactgat gctgtcatca
     1141 aagtggttaa ccaaaaggaa acaccagtag tctttattct gtggggtggc tatgctcgaa
     1201 agaaaaagtc gctaattgat aatcctattc atcatattat tgagagtcct catccgagtc
     1261 ccctatctgc ttatcgtggc ttctttggta gcagaccttt ttctagaaca aatcatttct
     1321 tagaagaaga aggaataaat gagattgatt ggttaaatta gaagggaaat ctactctcga
     1381 aacattgtta gtcttataat aaaaaaccgc tactagcggt tttttattat ttatttcttt
     1441 gtttacttag gtttaagcca aacggaacca aattttcttg cctttttcga atacgtttga
     1501 tattatcctg atgtcggata ataatcgtga gggccattaa gataaccaca gtggtaaata
     1561 tccagtcgta gtccgttaat ataaaaccta ctaatggaaa aattaagaca ctcaggatac
     1621 ctacgacagc tactgtaata ctggatagcg aaatcatgct aaaaagataa agcgttaata
     1681 aaaagataac taataagtaa aggaaaaaag aaggtgcaaa tcctagcaaa accccagcgc
     1741 ttgtagcaac ggctttccca cctttgaatt gtgcaaaaat agggaaagtg tgacctataa
     1801 tagcaaaaaa accgataaaa aatggagata ctgtcgttac acctaacatg ataggaataa
     1861 gtgttgctag agtccctttt aaaatgtcaa tagtcaaagt gacaatgcca gcttttacac
     1921 ctaaaatacg gaaggtattc gtggtgccag tattaccact accgtgttga cgaagattga
     1981 cttggtaaaa atatttacca atccaaagtc cagtttggat agagcctaac agataggcaa
     2041 tgataatcat aattataata ctcataccct tattataaca taagcataaa agtctttttg
     2101 ctatttttaa aaaaataatt ttgcaaaaac agcaaaaaac ttgtaatata aaaaagatga
     2161 aaaaatttgg aggtcacttt ggctaaacaa gatataacag tgacgaatta tggcgacgat
     2221 gctattcaag tcttagaagg attagatgct gttcgtaagc gtccagggat gtatataggt
     2281 tccacagatg gaactggttt acatcattta gtgtgggaaa ttgtagataa tgcagttgat
     2341 gaagctctat caggttttgg aaatcgcatt gatgttatta ttaacaaaga tgggagtata
     2401 actgttacag accatggacg cggtatgcca actggaatgc atgctatggg aaaacctact
     2461 gttgaagtca tcttcacggt gcttcatgca ggtggaaaat ttggacaagg tggttataaa
     2521 acatcaggtg gtctccatgg tgttggatca tctgtagtta atgctttgtc ttcttggtta
     2581 gaagttgaaa tcattcgcga cggagctatt tacagacaac gttttgaaaa tggtggtaag
     2641 cccgttacaa ccctgaaaaa aatcggcacg gcaccaaaat caaaatctgg gacatctgtc
     2701 agctttatgc cagatcagtc agtattttca acgatagatt ttaagtttaa tacaattgca
     2761 gagcgcttaa aggaatctgc ctttctctta aaaaatgtta ctttaacctt aacagataat
     2821 cgttcagaag aagcagagca tctagaattt cattatgaaa acggtgttca agattttgta
     2881 gagtatttaa atgaagataa agaaacgttg acaccaatca tgtttttcga aggtgaagaa
     2941 caagaatttc atatcgaagt tgctttgcag tataatgatg gtttttcaga taatattctt
     3001 tcttttgtca ataatgttcg tactaaagat ggtggaacac atgaaactgg cttgaaatca
     3061 gctattacaa agtctatgaa tgactatgca cgtaaaacag gacttcttaa agaaaaagat
     3121 aaaaaccttg aaggttctga ttatcgtgag gggctatcag ccattttatc tattcttgta
     3181 cctgaggagc acttgcaatt tgaaggccaa actaaagata aactaggaag tcccttagca
     3241 cgaccaattg ttgatggtat tgtttctgaa aagttaactt actttcttat ggaaaatgga
     3301 gatttagcat ctaatttaat ccgtaaggca ataaaagcac gtgatgcgcg tgaagctgca
     3361 cgtaaagctc gtgatgaaag tagaaatggg aaaaagtcga aaaaagataa aggtctactt
     3421 tctggaaaat taacccctgc acaatcaaaa aatgctaaga aaaatgaact ctatttagtc
     3481 gaaggagact ctgcaggtgg ctctgctaag caaggtcgtg atagaaagtt tcaagctatt
     3541 ttaccgttgc gaggaaaagt attgaataca gctaaggcaa agatggctga tattataaaa
     3601 aacgaagaaa ttaatacaat gatccatacc attggtgcag gtgttggtcc tgattttaat
     3661 attgacgata ttaattatga taaagttatc attatgaccg atgctgatac tgacggagct
     3721 catattcaga cactattatt aactttcttt tatcgctata tgagacctct tgttgaagag
     3781 ggacatgtct atattgcttt gccacctttg tataaaatgt caaaaggaaa aggtaagaaa
     3841 gaaattgttg agtatgcttg gacggatata gagttagaag aattacgaca aaaatttggt
     3901 aaaggctcac tattacaacg atataaaggg cttggggaaa tgaatgccga tcaattgtgg
     3961 gaaaccacta tgaaccctga gacaaggaca ttaattcgtg taacaattga agatttagca
     4021 agagcagaac gtcgagttaa tgtcttgatg ggtgataaag ttcctccaag acgccaatgg
     4081 attgaagata atgtcaagtt tactttagaa gagaatacag tattttaata aagagtcttg
     4141 gttattcatg ttttatagtg tacaagtaat attgtatgcg ttagacaaga gagccaatca
     4201 ttcatttcaa ataagtggaa aaataatact aagccactcc aactaggaga aaattaaaaa
     4261 tatgtctaat attcaaaata tgtcccttga ggacattatg ggagagcgtt ttgggcgcta
     4321 ttctaagtat atcattcaag aacgggcgtt gccggatatt cgtgatggct taaaacctgt
     4381 tcaacgtcga atcctttatt caatgaataa agatggtaat acttttgaaa agggatttcg
     4441 caaatctgca aaatcagtcg gtaatgttat ggggaatttc catcctcatg gggatttctc
     4501 tatctatgac gcgatggttc gtatgtctca agattggaaa aatcgtgaga cattgattga
     4561 aatgcacgga aataatggtt ctatggatgg agatcctgcg gcggctatgc gttatacgga
     4621 agcccgtcta tcagaaattg ctggttatct tcttcaagat attgataaga ataccgtacc
     4681 ctttgcctgg aatttcgatg atacagagaa agaaccaact gttttaccag ctgcctttcc
     4741 caatctttta gtcaacggag caacaggaat atctgcaggc tatgcaacag atattccacc
     4801 acataattta gcagaagtca ttgatgctgt cgtgtacatg attgatcacc ctaaagctaa
     4861 attagataaa ttaatggaat ttctacctgg tccagatttt ccaactggcg ctatcattca
     4921 aggaaaagat gaaattcgta aggcatatga gactggtaag gggagagtag cggttcgctc
     4981 gcgaactgct attgaaacct taaaaggtgg taagaaacaa attattgtta ctgaaattcc
     5041 ttatgaagtt aataagtcag ttttagtaaa acgaatcgat gatgttcgtg ttaataataa
     5101 agttccagga atagcagagg tgcgtgatga gtctgataga gatggtcttc gtatcgctat
     5161 tgagctcaaa aaagaagccg atgaaacaat tgtcttaaat tatcttttta aatatacaga
     5221 cctacaagtg aattataatt tcaatatggt tgctattgat gactacaccc ctaagcaggt
     5281 gggtctatca agaatattaa ctagctatat cgctcaccga agagaaatta ttattgctcg
     5341 ttcaaaattt gataaagaaa aagccgaaaa acgtctccat attgttgagg gactgattag
     5401 ggttctttct attttggatg aagtgattgc attaattcgt gcttctgaga ataaagctga
     5461 tgcgaaagag aatttgaagg tcagctatga gttttcagaa gcccaagcag aggctatcgt
     5521 cacattgcaa ttatatcgtc tcacaaatac tgatattgtg acgttgagag aagaagaaga
     5581 agaacttcgt caacaaatca caatgcttaa ggcaattatt agtgatgaac gtacgatgta
     5641 taacgtaatg aaacgtgagt tacgtgaggt caaaaagaaa tttgccaata ctagacgttc
     5701 tgaacttcaa gaattagctg aaactattga aatcgatact gcaagtttaa ttatcgaaga
     5761 ggacacatac gtcagtgtta ctagaggcgg atatgtaaaa cgcaccagtc ctcgctcttt
     5821 caatgcatca actgttgatg aacttggtaa gcgagaagat gacgaattga tatttgtgag
     5881 caatgcaaag actacccaac atttattgat gtttactaac cttggtaatc ttgcttatcg
     5941 tccagttcat gaattagctg atattcgatg gaaagacgtt ggtgaacatt tatcacaaaa
     6001 tcttgttaac tttgcttcaa atgaagaaat tatttatgct gaattagtag atgattttac
     6061 taaagaaact tattttgctg tcactagatt agggcaaata aagcgttttg aacgtcaaga
     6121 aatcagtcca tggcggacat ataagtcaaa aacagctaaa tatgctaagt taaaaaatgt
     6181 agatgattac gttgtgactg ttgctcctat ccaattagag gatgttatac ttgtcactta
     6241 taatggttat gcgttgagat ttagtataaa tgatgtacca gtcgttggta gtaaagccgc
     6301 aggtgtaaaa gcgatgaatt taaaagatag tgatcacatc gtatctgcat ttatagctaa
     6361 caccacaagt ttatatttat taactcatag aggaagtctt aaacgaatgg ccattgatgt
     6421 tatccccaca actagtcgtg ctaatcgagg acttcaagtt cttcgtgaat tgaaatccaa
     6481 accgcatcgc gtttttaaag caggacctgt atatttagag gattcatctt ttgaatttga
     6541 tttatttagt tcagtatcga atcatgaagg agatactttc gtacttgaga ttatgtctaa
     6601 aacaggaaaa gtttacgatg ttgatttaag tcaatggagt ttttctgaac gtacaagtaa
     6661 tggctctttt gtatctgata agatttctga cgaagaagta ttctcagtta aaattaagta
     6721 aaacacattt gtgtttttac tagtttggta atttttctga attttacaaa taatattgaa
     6781 aaaataatga taatagcgta caatagttag aaacccaaag aaagaggtac cttatgacag
     6841 taaatttaga ttgggataat ttaggttttg cttacagaaa attaccattc cgatacattt
     6901 cgcattttaa agatgggaaa tgggatgatg gcaagttgac agatgatgca acgctacata
     6961 tttcagaaag ttctcctgcc ctccactatg ggcaacaagc ttttgaagga ttgaaagctt
     7021 atagaactaa agatggctct attcagttat ttcgtccaga tcaaaatgca gaacgacttc
     7081 aaagaacagc agatcgtctt ttaatgccac atgttccgac agacaaattt attgcagcgg
     7141 ttaaatctgt tgtacgcgct aatgaggagt ttgttccacc gtatggtaca ggggcaacat
     7201 tatacattcg tcccttatta attggtgtgg gagatattat tggagttaaa cctgctgaag
     7261 aatatatttt tacggtattt gcaatgccag taggtagtta ttttaaaggt ggtttaacac
     7321 caactaactt tattgtttcc aaagaatatg atagagcagc accgaatggt acaggagctg
     7381 caaaagttgg tggtaactat gctgctagtc ttcttcctgg gaaatacgct catgagaaac
     7441 aattttcaga tgttatctat cttgatccag cgacacatac taaaattgaa gaggttggag
     7501 cagctaattt ctttggaatt actaaagata atcagtttat cacccctttg agtccttcaa
     7561 ttttaccttc aattactaaa tattcattgc tttaccttgc taaagaaagg tttggtatgg
     7621 aagcgattga aggagatgtc tttgtcgacg aattagataa attcacagaa gctggcgcat
     7681 gtggtacagc cgcagttatt tcaccaattg gaggaattca aaacggagat gacttccatg
     7741 tcttctacag tgagacagaa gttggtccag cgacacgtaa actatatgat gagttagtag
     7801 ggatccagtt tggtgatgtt gaagcaccag aagggtggat ttataaagta gattaagtct
     7861 atactaaaag cttgctcttg caagctttta gtattttgtg tacaataaaa aacaataaaa
     7921 gagatattgg agttaatagt gagtaaaaaa gataagaaaa ttgaaattca aatttcagac
     7981 gctcaagtaa cagttaatgg aactaaggtt gatggatacc aactcgttat tggaaaaaaa
     8041 attgtaggtc aaattgcaga gctagattct aaatttgctg tgattaaagg tgagcaagtg
     8101 agtggattct ataaaaattt agatcaagct atgggaaata ttattgaaga atataatctt
     8161 aatcgctaat atttatttaa aaagcttgcg tgctaacaac atctgtggta gaatagtcag
     8221 tgttgttacg gaaagatagc gaagaggcta aacgcggcgg actgtaaatc cgctccttcg
     8281 ggttcggggg ttcgaatccc tctctttcca ttatttgggg tatagccaag cggtaaggca
     8341 agggactttg actccctcat gcgttggttc gaatccagct accccagtca aaggtatcaa
     8401 acttttgttt gacaccgtca aagagatatt tgttgtcctt gcgggttacc ttgagaaaat
     8461 atattgttat caaaagctag ctcggctagc ttttgttgga ggatttttta gaatgaatga
     8521 atttgaagat ttgctaaaca gtgttagcga agtgaaccct ggtgacgttg tcactgcgga
     8581 agttttaaca gttgataacg gtcaagcaaa cgttgttatc gatggtactg gtgttgaagg
     8641 tgtcttgaca cttcgtgaat tgactaacga tcgtgatgct gatatcaatg attttgttaa
     8701 agctggcgac acagttgaag tacttgttct tcgtcaagtt gtaggtaaag atactgatac
     8761 agtaactttc cttgtatcta aaaaacgctt ggaagctcgt aaagcatggg acaaacttgt
     8821 tggtcgtgaa ggtgaagttg ttactgttaa aggtacacgt gctgttaaag gcggactttc
     8881 agttgaattt gaaggacttc gtggtttcat cccagcttca atgattgata ctcgttttgt
     8941 acgtaacact gaaaaatttg ttggacaaga atttgatgct aaaatcaaag aagttgatgc
     9001 agcagaaaat cgcttcatcc tttcacgtcg tgaagttgtt gaagaatctg cagctgcagc
     9061 acgtaaagaa gttttctcaa acattgaagt aggttcagtt gtaactggta aagttgctcg
     9121 tttaacaagc tttggtgctt tcattgacct tggtggtgtt gatggacttg tacacgtaac
     9181 tgaattatca catgagcgca atgtttcacc taaatcagtt gtaactgttg gtgaagaagt
     9241 tgaagttaaa gtactttcaa ttgatgaaga agctggtcgt gtatcacttt cacttaaagc
     9301 aactacacct ggcccatggg atggcgttga acaaaaactt gctgctggtg atgttattga
     9361 aggtaaagta aaacgcttga ctgactttgg tgcttttgtt gaagtgttac ctggcattga
     9421 tggactcgta cacatttcac aaatttcaca caaacgtgtt gaaaatccta aagacgtatt
     9481 atctgcagga caagaagtta ctgttaaagt tcttgaagtt aattcagatg cagaacgtgt
     9541 atcactttca atgaaagctc ttgaagagcg tccagcgcaa gcagaaggtg aaaaagaaga
     9601 aaaacgtcaa tcacgtccac gtcgtccacg tcgtcaagaa aaacgcgact acgagcttcc
     9661 agaaacacaa actggattct ctatggctga cttattcggt gatattgaat tataataatt
     9721 aaaagacttg ctttagcaag tctttttgtt tttgttgata aagtatgcta taatgtaaca
     9781 tgtactacca tctttagtgt aatggatatc acacaagatt ccggttcttg ggataggggt
     9841 tcgattccct taagatggat aatagatgct ccgtaaagct ttatgttata gggtttatat
     9901 tagtttatgc cccaaattcg ccccaaataa ttccctaata atttcattgt tcttgttttt
     9961 tgtatcttcc agcaaatgag catacgtgct tagtgtgata tttaaatctt tgtgtccaac
    10021 tatcttggaa atagcaaaca agtcgattcc ttctgatatt aaatgagaca cataagtatg
    10081 tcttagggaa tgaatgtgta tatttctgcc aactaatttt tttagtgtct tgttgacagc
    10141 tgtatttgat attccttcaa atatccttgt ttctgttttg aataacaacc tatattgatc
    10201 tagcaatttg atagttgtgt tatttattgg tacatatcta attgagtgtt tatttttagt
    10261 tggttgaaat cctttatcac ttccaaacac tttgtaagtc ttgttgacat gtagtagcaa
    10321 ctcatctcta tctatatcgc tccaagtcaa tcccatagcc tctgaaaatc ttaatccagt
    10381 tgttgctatt aaataaatac aaaaatgcga cttatacttc acttca
//
LOCUS       DATRMA010000066        10361 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.066, whole genome shotgun sequence.
ACCESSION   DATRMA010000066 DATRMA010000000
VERSION     DATRMA010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10361
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.066"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            <1..224
                     /locus_tag="VBC28_001591"
     CDS             <1..224
                     /locus_tag="VBC28_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="surface protein"
                     /protein_id="HEO6815560.1"
                     /translation="NKDSVSKAQFGNYNINGYIMDMQYNKQEYNALANTPKGQKTKKT
                     FKPMTLVILFAVALFIFYITAIRFVFKRM"
     gene            249..1073
                     /locus_tag="VBC28_001592"
     CDS             249..1073
                     /locus_tag="VBC28_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LicD family protein"
                     /protein_id="HEO6815561.1"
                     /translation="MKEMTVSEIREVQLEMLAYIDKVARDNKIEYSLGGGSLLGAMRH
                     KGFIPWDDDIDLMLERSQYERLMKALADANNSDFKLLHHSVEKNLWPFAKLYHTKSMY
                     LSKTDRIHPWTGIFIDIFPLDRLPESAEERQRFFKKVHSAAANLMCTTYPNFASGSRK
                     LYANARLILGLPRFIAYHGQAKKRAEIVDQVMETYNNQEVPYMGYTDSRYRLKEYFPR
                     EIFSEYEDVMFENIKTRKIKNEHAYLNQLYGGSYMELPPESKRESHSYYTWYWKEG"
     gene            1073..1795
                     /locus_tag="VBC28_001593"
     CDS             1073..1795
                     /locus_tag="VBC28_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEO6815562.1"
                     /translation="MMNIGVIFAGGVGRRMNTKGKPKQFLEVHGKPIIVHTIDIFQNT
                     EAIDAVVVVCVSDWLDYMNNLVERFNLTKVKAVVAGGETGQMSIFKGLEAAEQLATDD
                     AVVLIHDGVRPLINEEVINANIQSVKETGSAVTSVRAKETVVLVNDSSKISEVVDRTR
                     SFIAKAPQSFYLSDILSVERDAISKGITDAIDSSTLMGMYNRELTIVEGPYENIKITT
                     PDDFYMFKALYDARENEQIYGM"
     gene            1795..2853
                     /locus_tag="VBC28_001594"
     CDS             1795..2853
                     /locus_tag="VBC28_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEO6815563.1"
                     /translation="MMIKFSNKIVQEDLALISNQNYINWERIANKGVLISGSNSMLAS
                     YMVFLLAYLNETRNYQTQIIATARNIEKARDKFSDLVGKDYFTLIPYDVEERLEYDGK
                     VDYIIHAASNASPTAILSNPVSIIKANTIGTLNLLDFAKEKTIENFLFLSTREVYGTS
                     IKEVIDEEAYGGFDILATRACYPESKRMAETLLQSYYDQYKVPFTIARIAHSFGPGME
                     LGNDGRIMNDLLSNVIDGKDIVLKSSGTAERAFCYLADAVSGLFTILLNGEVGQAYNV
                     ANEDQPIMIKNLAQKLVDLFSDKNISVVFDIPKTMSAGYSKMGRTRLTMAKLEALGWK
                     REVSLESGILKTVQAFEE"
     gene            2855..3790
                     /locus_tag="VBC28_001595"
     CDS             2855..3790
                     /locus_tag="VBC28_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEO6815564.1"
                     /translation="MVKTAVLMATYNGEKFISEQLDSIRQQTLKPDYVLLRDDCSTDE
                     TVNVVNNYIAKHELEGWKIVKNDKNLGWRLNFRQLLIDVLAYEVDYVFFSDQDDIWYL
                     DKNERQFAIMSDKPQIEVLSADVDIKTMSTEASVPHFLTFSSSDRISQYPKVYDYQTF
                     RPGWTIAMKRDFAQAIADNWTFGANISHDNLIETTASIIGNGYNLNESVGVHIRYGGN
                     ASGNSKFVLNWKSPKTQHITGLKTFVDFYETMQKVVNKYQNDVALNQRLAFEKERLEA
                     SKLSLIHNSAFVIKNWRLYNSNSGRMRDLLFAFKK"
     gene            3799..4746
                     /locus_tag="VBC28_001596"
     CDS             3799..4746
                     /locus_tag="VBC28_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEO6815565.1"
                     /translation="MVKTAVLMATYNGQGFIHDQLDSIRNQTLRPDYVLMRDDGSTDD
                     TVKVVEDYIKEHRLDGWSITSNDKNLGWRLNFRQLLIDVLAYEVDYVFFSDQDDTWYH
                     HKNKMQVDIMEERQDINLLSADIDIKKLSEEATIPNNFKFNGEETISKYPLDFSYHNY
                     RQGWTFCLRKSFVDQVMTFYSDNLILSHDNLFAGVSGVIGSGYNLNRSVGEHKRHAEN
                     ASGNILNMSSSYERHLQELYFVLSYYIILVPFLEKSSSSQLREAKAYLDFNQQRYKNA
                     QNKDFVAILKQMMTKKRFYDSFSNRIRDLVFLLKDIKRS"
     gene            4748..6112
                     /locus_tag="VBC28_001597"
     CDS             4748..6112
                     /locus_tag="VBC28_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2142 domain-containing protein"
                     /protein_id="HEO6815566.1"
                     /translation="MNLKKVRLFEKFFLDKNLHKTYFILGLTITIVLSLLMPFFNEPD
                     GQYHLAVSGRISNNIIDTSYYGEYNIDSGMRRQSTSYRDGTRFEKYYLNKANFVDGNH
                     LPREINYSKHNFVFWGHLVPAVGLFLGKFVYPSMGVMITVARLFSSFVSITLVALIIR
                     YVKKGKLIYFAIFLSPVVLNSFASLSYDSTGYIVFGAFLSLLINLLVDKSLTKNLSIA
                     LIGLILLVIVACKQNYWLFLLLVIPVLLQVNNPKLMGYNQKLSHLFQGLKSKKLFWLC
                     LTFLSLAIVYVLTARHGGVLAVLRRYVMTFGYNYAGINILSNDITSWLASPYPSQNFI
                     PTWVSSVWYLMVFLVWFSEEKFVKSKNMGFLFLASFFVGILGVFFIMLEYRGAPTSYI
                     EGVQGRYFTVTLLLVQVFASSIPARLNEAGRKVVPYALTGLVIVSNALLLFDTVISLI
                     MRRN"
     gene            6112..7536
                     /locus_tag="VBC28_001598"
     CDS             6112..7536
                     /locus_tag="VBC28_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEO6815567.1"
                     /translation="MKLLKNMFYNTSYQLLTLLLPLVTVPYVSRVLSPQGIGINAYTS
                     SIVMYFTLFGALGISLYGNREIAFVQSNKYKRSKIFWELVVLKLASVSIATLLFFGFV
                     LLTNEWQLFYLIQGINLLATATDISWYFIGVEDFKIIVIRNTIVKLITVVLTFLVVKT
                     PDDLALYMFLIAFASLLGNLTVWHHLKHEIIKIPFSRLDILIHLRPTLMLFLPQITMQ
                     IYLSLNKSMLGAMDSVVSAGYFDQSDKIIRILFTIVSAIGGVFLPRLSSLFSSGKEKQ
                     AKALLLKLVDLSNAISMLMIAGVVGVSSTFAVFFFGKGYEAVGPLMAVESLMIICISY
                     GNALGTQYLLASRRTKAYTMSAVIGLVANVVLNILLIPILGAMGAIISTVITEFIVSL
                     YQAISLRDVFTFKELTRGMLRYLIAATLSGAVLYYINTQMSVSLVNYVIQSLVAVTIY
                     VGIVFITKAPVIQLFRDFRKEHRT"
     gene            7533..8381
                     /locus_tag="VBC28_001599"
     CDS             7533..8381
                     /locus_tag="VBC28_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000875978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEO6815568.1"
                     /translation="MKYLAGIVTFNPNIERLDQNIRAIYPQVSHIYIVDNGSKNKEEI
                     SQLVADYNEEGHLTVDYLTENKGIAYALNCIGQFAVAQEFDWFLTLDQDSVVLGDLID
                     NYENYLHLPKVGMLSCLYQDMNRENLVMQEFDYKEIEECITSAALMKTSVFEETSGFA
                     EEMFIDFVDSEMNYRLSEMGYKTYQVNFIGLLHEIGHSSRVKKFGHVFHVLNHSPFRK
                     YYMIRNAIYIIKKYGKKKRYKYLVFMRNEFVRVLVAEEQKSKKIVAMIKGLKDGLLMK
                     VDKYGK"
     gene            8371..9510
                     /locus_tag="VBC28_001600"
     CDS             8371..9510
                     /locus_tag="VBC28_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEO6815569.1"
                     /translation="MENKVKTVAVFSGYYLPFLGGIERYTDKMTADLVKRGYRVVIVT
                     TNHGDLPIIDEDKGRKIYRLPTKNIVKQRYPIINKNREYNTLMKYVSDENIDFVICNT
                     RFQLTTLEGLSFAKNHHLPSIVLDHGSSHFSVNNRFLDFFGAIYEHLLTARVKHYRPD
                     FYAVSKRSVEWLKHFNIEAKGVIYNSVSESLGSDFAGTAYLEKSADDIFITYAGRIIK
                     EKGIELLLEAFSMSQYSENVYLQIAGDGPELAHLKEKYQSKQINFLGKLNFEQTMSLM
                     AQTDIFVYPSMYPEGLPTSILEAGLLSSAIIATDRGGTVEVIDSPELGIIMEENTQSL
                     HESLDLLVKDKALREKLQQNIAKRIKEHFTWEKTVEKLDYIIQKN"
     gene            complement(9562..>10361)
                     /locus_tag="VBC28_001601"
     CDS             complement(9562..>10361)
                     /locus_tag="VBC28_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEO6815570.1"
                     /translation="DSFKINLKKDDIDYIFLAYLTSHNSFSNPNWTEKRIDNVIAIFE
                     NYPKFQKLLQPLKDALPLSGSYHDELVKVAIFFFRTFILGLNQLIPETISFPSWNYHR
                     HDKLTTILRPIITNWLSKIGEYTFKEQHFLLLCTHLERIIKNHIPPIQIAVLTTDFIN
                     NQILTECLLQRFSSKKIHFHPYYLLTDDLSNITNLNPDIIITNKKLSPFIKHEISSES
                     LITYIDLINTSDQINQIQKIISSIQEEKYCKLFAKTNEITNRSSYNS"
BASE COUNT         3394 a         1481 c         1979 g         3507 t
ORIGIN      
        1 taaataaaga tagtgtttct aaagcacaat ttggtaacta taatataaat ggttatataa
       61 tggacatgca gtacaacaaa caagaataca atgcacttgc aaacactcca aaaggtcaaa
      121 aaacaaagaa aactttcaaa cccatgaccc tagtgattct ttttgcagta gctttattta
      181 ttttctatat tacagctata agatttgtat ttaaaagaat gtgagttaac tgttagacag
      241 aggtaattat gaaagaaatg acggtctcag aaataagaga agttcaactt gaaatgttag
      301 cttatattga taaagttgcg cgtgataata aaattgaata ttctttaggc ggaggcagcc
      361 tactgggagc aatgagacat aaaggattca ttccatggga tgacgatatt gatttgatgc
      421 tagaaagaag ccagtatgag cgtttgatga aagcattagc tgatgctaat aatagcgatt
      481 ttaaattgct acatcattct gttgaaaaaa atctatggcc atttgccaag ctgtatcaca
      541 caaagtcaat gtacttaagt aaaacagata gaattcatcc ttggacaggg atttttattg
      601 atatcttccc acttgaccga ttaccagaga gtgcagaaga acgtcaaaga ttctttaaaa
      661 aagttcactc tgctgctgca aatttgatgt gtacgactta ccccaatttt gctagcggtt
      721 cccgaaagct ttatgcaaat gcaaggctaa ttttggggct accccgattc atcgcttatc
      781 acggccaagc taaaaaacgt gctgaaattg ttgatcaagt gatggaaaca tataataatc
      841 aagaggttcc ttacatgggt tataccgatt caagatatcg cttgaaagaa tattttccaa
      901 gagaaatttt ttctgagtat gaagatgtta tgtttgaaaa cattaaaacc cgaaaaatta
      961 aaaatgagca tgcttatctg aatcaattat atgggggtag ttatatggaa ttacctccag
     1021 aaagtaagag agaaagtcat agttattaca cttggtactg gaaagaaggt taatgatgaa
     1081 tattggagtt atttttgcag gtggtgttgg tcgtcgcatg aataccaaag gtaaaccaaa
     1141 gcaattccta gaagtgcatg gtaaacccat tattgtacac acaattgata ttttccaaaa
     1201 tacagaagct attgatgctg tagtagttgt ctgtgtatca gactggctag attatatgaa
     1261 taatctggta gagcgtttca accttacgaa agtaaaagca gttgttgcag gtggagaaac
     1321 tggccaaatg tcaattttta aggggcttga agccgctgaa cagctggcta cagatgatgc
     1381 cgttgtttta attcatgacg gagtacgtcc attgattaat gaagaagtta ttaatgctaa
     1441 tatacagtca gtaaaagaaa caggaagtgc ggtaacttca gttcgtgcta aagagacagt
     1501 tgttctagtc aatgattctt ctaaaatatc tgaagttgtt gatcgtactc gttccttcat
     1561 tgctaaagcg ccacaaagtt tttatctatc agatatcctt tctgttgaac gtgatgccat
     1621 tagcaaaggg attactgatg caattgattc tagtactttg atgggtatgt acaatcgtga
     1681 actcactatt gttgagggac catatgaaaa tatcaagata acaacaccag atgattttta
     1741 tatgtttaaa gctttgtatg atgctagaga aaatgaacaa atttatggga tgtaatgatg
     1801 attaaattta gtaataaaat tgtacaagaa gatttagcac ttattagtaa ccaaaattat
     1861 attaattggg aaagaattgc taataaaggc gttttaataa gtggttctaa tagtatgtta
     1921 gcgagttata tggtattttt actagcctat ctcaatgaga caagaaatta ccaaacgcaa
     1981 attattgcga cagctagaaa cattgaaaaa gcccgtgata aattttcaga tttagttggg
     2041 aaagattatt tcaccttaat tccttatgat gtagaagaac gcctagagta tgatggaaaa
     2101 gtggattaca ttatccatgc agcaagcaat gctagtccaa ctgctatttt gagtaatcct
     2161 gttagcatca taaaagctaa tacaattggt acattgaatt tattagactt tgcaaaggaa
     2221 aaaacaattg aaaacttctt gttcttatca acgcgtgaag tatatggaac atctattaaa
     2281 gaagtgattg atgaagaagc ttatggtgga tttgatattt tagctactag agcttgttac
     2341 ccagaaagca aacgtatggc tgaaactcta ctgcaaagtt actatgatca atataaggta
     2401 ccctttacta ttgctcgtat tgcacattca tttggtcctg gtatggagct tggtaacgat
     2461 ggtcgtatta tgaatgatct tttatcgaat gtcattgatg gcaaagatat cgttttaaaa
     2521 agtagtggta cagcagagcg tgctttctgt tatttagcag atgctgtatc agggttattt
     2581 actattttat taaatggtga agttggtcaa gcttacaatg tagcaaatga agatcaaccc
     2641 attatgatta agaatttggc acaaaagtta gttgatctct tttctgataa aaatatttca
     2701 gtagtattcg atattcctaa aactatgagt gcaggttata gcaaaatggg gagaacacgt
     2761 ctcactatgg ccaaattaga agcgttggga tggaagcgag aagtttctct tgaatctgga
     2821 atattaaaaa cagtacaagc atttgaggag taatatggta aagacagcag ttttaatggc
     2881 gacatacaat ggcgaaaaat ttatatctga acaacttgat tcaattcgcc aacagacatt
     2941 aaaaccagat tatgtattat tgagggatga ttgttcaacg gatgaaacag tcaatgtcgt
     3001 caataactat atcgcaaaac atgagttaga aggctggaaa attgttaaaa acgacaaaaa
     3061 cttaggctgg cgtttaaatt ttcgtcaatt acttattgat gtgttagcct atgaggttga
     3121 ctatgtcttt tttagtgatc aagatgatat ttggtatctt gataaaaacg aacgacagtt
     3181 tgccattatg tcagataaac ctcaaattga ggttttgagt gcagacgttg atatcaaaac
     3241 gatgtctaca gaagccagtg ttccacattt tctaactttt tcttctagtg atagaatcag
     3301 tcagtatcct aaagtatatg attatcaaac attccgtccc ggatggacca ttgctatgaa
     3361 gagagatttt gcgcaagcta tcgctgataa ttggactttt ggagctaata tttcgcacga
     3421 taatcttatc gagacaactg ccagtattat tggaaatggt tataacctta acgaaagcgt
     3481 aggagttcat attcgatatg gaggaaacgc aagtggtaat agcaaatttg ttttgaattg
     3541 gaagtcgcct aaaacacaac atatcacagg attaaaaaca tttgttgatt tttacgaaac
     3601 catgcagaag gttgtcaaca aatatcaaaa tgatgtcgct ttaaatcaac gcttagcctt
     3661 tgaaaaagaa agattggaag cttctaaatt gtcattaatt cataatagtg cttttgtgat
     3721 taaaaattgg cgactctata actctaatag tggacgtatg agagatttat tatttgcgtt
     3781 taaaaagtaa ggaataatat ggtaaaaaca gcagttttga tggcaacata taatggtcaa
     3841 ggtttcatcc acgatcagct agattcaatt agaaatcaaa cgttgagacc agactatgtc
     3901 ttaatgaggg acgatggctc tactgatgat actgtaaagg tggttgaaga ctatataaaa
     3961 gagcaccgtt tagatggttg gtcaataact agtaatgata aaaatttagg ttggcgttta
     4021 aattttcgtc aattacttat tgatgtgtta gcctatgagg ttgactatgt cttttttagt
     4081 gatcaagatg acacatggta tcatcataaa aataagatgc aagttgatat tatggaagaa
     4141 cgccaagata taaaccttct tagcgccgat attgatatca aaaagttatc agaagaggct
     4201 actattccca ataattttaa gttcaatggt gaggaaacaa tttctaagta tccattagac
     4261 ttttcatatc ataactatcg tcaaggttgg actttttgtt tacgcaagtc gtttgttgat
     4321 caagtaatga ctttttacag tgataattta attttgtcac atgataatct ttttgcaggg
     4381 gtatctggtg tcattggttc aggttacaat ttaaacagaa gtgttggtga acataagcga
     4441 catgctgaga atgcaagtgg gaatatcttg aatatgagct caagttacga aaggcattta
     4501 caagaactgt attttgtttt gagttactat atcatccttg taccattttt agaaaaaagt
     4561 agctctagtc aattaaggga agctaaagct tatctggatt ttaatcagca acgttataaa
     4621 aatgctcaaa ataaagattt tgtagcaata cttaaacaaa tgatgactaa aaaacggttc
     4681 tatgatagct tttcaaatcg cattcgtgac ttggtgtttt tattaaaaga tataaaaagg
     4741 agttaatatg aacctcaaaa aagttcgtct atttgaaaag ttttttctag acaagaactt
     4801 gcataaaaca tattttatat taggcttaac gattactatt gtattgagcc tattaatgcc
     4861 atttttcaat gaacctgatg gacaatacca tttagctgtg tctggtcgta tttcaaataa
     4921 cattattgat acttcttact atggagaata taatattgat tcaggtatga gacgacaatc
     4981 gacctcctat agagatggta ctagatttga aaaatactat ttaaataaag ccaattttgt
     5041 tgatggtaat catttgcctc gtgagataaa ctattctaaa cataactttg ttttttgggg
     5101 tcatcttgtg ccagcagttg gcttattttt aggcaaattt gtgtatcctt ctatgggggt
     5161 aatgattaca gtagcaaggt tatttagttc ctttgtctca atcaccttgg ttgccctcat
     5221 tatccggtat gtgaaaaaag gaaagttaat ctactttgct attttcctca gtcccgtagt
     5281 tctaaattca tttgctagtc tatcttatga ctcgactgga tatattgtat ttggagcatt
     5341 tttatcgctt ttgataaatc ttttagttga taaatcgttg actaaaaatc ttagtattgc
     5401 tcttatagga ttgattcttt tagtcattgt tgcatgtaag cagaattact ggctgttttt
     5461 attgctagta ataccggtat tattgcaggt taacaatcct aaattaatgg gctataatca
     5521 gaagctatca catttatttc aaggtcttaa atctaaaaag ctattttggc tatgtctaac
     5581 ctttttgtca cttgctatag tttatgttct gaccgcaaga catggtggtg tccttgctgt
     5641 tttaagacgt tatgtaatga catttggcta taattatgct ggtatcaata ttttaagtaa
     5701 tgacattact tcctggcttg cctctccata tccaagtcag aattttattc ccacttgggt
     5761 atcatcagta tggtatctca tggtctttct agtatggttt tctgaagaaa aatttgttaa
     5821 gtcaaaaaat atgggatttc ttttcttagc tagtttcttt gtgggaattc ttggtgtctt
     5881 ttttatcatg ttagaatatc gtggagcgcc aacaagctac attgaaggag ttcaaggacg
     5941 ttactttact gttacattgc ttttggtaca agtttttgca tcaagtattc cagcgcgtct
     6001 aaatgaggca ggaaggaaag tggtgccgta tgcgttaact ggcttggtaa ttgtatcaaa
     6061 tgccttattg ttatttgata ctgttattag tttaattatg aggagaaact aatgaaactt
     6121 ttgaaaaata tgttttataa tacctcatac cagttattga ctttgttgtt acctttagta
     6181 acagttcctt atgtttcccg tgtactatct cctcaaggca tcggtattaa tgcctatacg
     6241 agctccattg tcatgtactt tactttattt ggtgctttag ggatatcttt gtacggtaat
     6301 cgtgagatag cttttgtgca gtcaaataag tataagagaa gtaagatatt ttgggaatta
     6361 gttgtattaa aacttgcctc tgtatctata gcaacgttat tattctttgg atttgtctta
     6421 cttacaaacg aatggcaatt attctattta attcaaggta ttaatttatt agcgactgca
     6481 actgatattt cgtggtattt tattggagtt gaagacttta agataattgt cattcgtaat
     6541 accattgtta agcttattac agttgttttg accttcttag ttgtgaagac gccagatgac
     6601 ctagcacttt atatgttttt aattgcattt gcatcattac tcggaaattt aacagtctgg
     6661 catcatttaa agcatgaaat catcaagata ccatttagta gattagatat cttaatacat
     6721 ctaagaccaa ctttaatgtt atttttaccc caaatcacca tgcagattta tctctctctt
     6781 aacaagagta tgctgggagc aatggatagt gtggtcagtg ctggatattt tgatcaatct
     6841 gataaaatta ttcgtatttt atttaccatt gtttcagcaa tcggaggagt tttcttacca
     6901 aggctctcca gtctattttc ttcaggaaaa gaaaagcaag ctaaggcttt acttttgaaa
     6961 cttgttgatt taagcaatgc tatctcaatg ttaatgattg cgggagtggt aggagtttcg
     7021 tcgacatttg ccgtcttctt ttttgggaaa gggtatgaag ctgtcggtcc gctaatggct
     7081 gttgagtctt tgatgatcat ttgcatttct tatggtaacg ctttagggac acagtatcta
     7141 ctggcttcaa gacgtacaaa agcttacaca atgtctgcag ttattggact tgtggcaaat
     7201 gttgtgctta atatactttt aataccaatt ttaggtgcta tgggagctat tatctctaca
     7261 gttattactg aatttattgt ctccctatat caagctatct ctttaagaga tgtatttact
     7321 tttaaagaat taacaagagg aatgctgcgc tatttaattg ctgctactct tagtggagct
     7381 gtactctatt atatcaatac tcaaatgtca gtatcattgg taaattatgt cattcaaagt
     7441 ttagtagcag taactatcta tgtcggtatc gtttttataa ccaaagcacc agtcattcag
     7501 ttatttagag attttagaaa agagcataga acatgaaata tttagcgggt attgtcacct
     7561 ttaaccccaa tattgaacgc ttagatcaga acattcgagc aatttatcct caagtaagcc
     7621 acatctatat cgtagataat ggctcgaaaa ataaggaaga aatttctcaa ttagtagccg
     7681 attataatga agaggggcac ctaactgttg attatttaac agaaaataaa gggattgcat
     7741 atgctttgaa ctgtatcggt caatttgcag tagcacaaga atttgattgg ttcttgactt
     7801 tagatcaaga ttcggttgtc ctaggtgatt tgatagataa ttatgaaaat taccttcatt
     7861 tacctaaggt cggtatgtta tcttgtttgt atcaagatat gaaccgtgaa aatttagtga
     7921 tgcaagaatt tgactataaa gaaattgaag agtgtattac ttcggctgct ttaatgaaaa
     7981 cgagcgtttt cgaagaaaca tctggctttg cagaagagat gtttattgat tttgttgact
     8041 ctgaaatgaa ttatcgtctg tcagagatgg gatataagac ttatcaggtt aattttattg
     8101 gtctgttaca cgaaattgga cactcttcac gagttaaaaa atttggccat gtttttcatg
     8161 tattgaatca ttcaccattt cgaaaatatt acatgatacg caatgcgatc tatattataa
     8221 aaaaatatgg taagaagaag cgatacaaat atttagtatt tatgcgaaat gaatttgttc
     8281 gagtgttggt agcagaagag caaaagagca aaaaaatagt tgctatgatt aaaggcttaa
     8341 aagatggctt attgatgaaa gtggataaat atggaaaata aggtaaaaac agttgctgtt
     8401 ttctcaggat attatctacc cttcctcgga ggtattgaac gttatacaga taaaatgaca
     8461 gcagatttgg ttaagcgtgg ctaccgtgtt gttattgtaa caactaatca tggtgattta
     8521 ccgattatag atgaagacaa gggtagaaaa atttaccgtt taccgaccaa aaatattgtc
     8581 aagcagcgct atcctattat taacaaaaat cgagaataca acacgctgat gaaatatgtt
     8641 tcagatgaaa atattgattt tgtgatttgt aatacacgtt ttcagttaac aacgctagaa
     8701 gggctatctt ttgcaaagaa tcaccattta cctagtattg ttttagacca tgggtcaagt
     8761 catttttctg tcaataatcg ttttttagat ttctttggag ctatctatga acatcttctc
     8821 acagcgcgtg ttaagcatta tcgtccagat ttttatgcgg tatccaagag aagcgttgaa
     8881 tggttaaaac attttaatat agaagctaaa ggagttattt ataattcggt atctgaaagt
     8941 ctaggctctg attttgctgg tacggcttac cttgaaaaat ctgctgatga tattttcatc
     9001 acttatgctg gtagaattat taaagaaaaa ggcattgaat tgctcttaga agcattttcg
     9061 atgtcacagt actctgaaaa tgtttattta cagattgcag gagacggacc tgaattagca
     9121 catttgaaag agaaatatca gtccaaacag attaactttt tggggaaatt aaactttgaa
     9181 cagactatgt ctttaatggc acagactgat atctttgttt acccttcaat gtatccagag
     9241 ggattaccaa cctctatttt agaagctggt ctactttcaa gtgctattat tgcaacagat
     9301 cgcggtggaa cagttgaagt tattgatagc cctgagttag gaatcatcat ggaagaaaat
     9361 actcagtcac tccatgaatc tcttgattta ttagtaaaag ataaggcatt aagagagaaa
     9421 ctacaacaga atattgctaa aagaattaaa gaacatttca catgggaaaa aacagttgaa
     9481 aagttggatt atattattca aaaaaattaa cattaaaaag ctagagcatt acacaatgct
     9541 ctagcttttt aatgttaatt tttaagagtt ataggagcta cgattagtta tttcattagt
     9601 ttttgcaaaa agtttgcaat atttttcttc ctgtattgat gaaataattt tttggatttg
     9661 attgatttgg tctgaagtat taatcagatc aatatatgta attaaactct ctgaagaaat
     9721 ctcatgtttg ataaagggag aaagcttttt attggtaata ataatgtctg gattaagatt
     9781 agtaatattg gaaagatcat cagttaataa atagtaaggg tggaaatgaa tctttttaga
     9841 agaaaatctc tgtaataagc attctgttaa aatttggtta ttaataaagt ctgtagttag
     9901 tacggctatc tgtatcggag gaatatgatt tttgatgatt ctttctagat gagtacaaag
     9961 gagaagaaaa tgttgttctt taaacgtgta ttctccaatt ttacttaacc aatttgtaat
    10021 tatcggtcgg agaatagtag ttaatttatc atgtctatga tagttccatg aaggaaatga
    10081 aattgtttca gggattaatt gatttaatcc taaaataaat gttcggaaaa aaaagatagc
    10141 aacttttacc aactcatcat gataggagcc agataaggga agagcatctt tgagtggctg
    10201 taataatttt tggaatttgg gataattttc gaaaatagct attacattat cgatacgctt
    10261 ctcagtccaa tttggattag aaaaagagtt atgggaagta aggtaagcaa gaaaaatata
    10321 gtctatatcg tcttttttta aattaatttt aaaggaatct a
//
LOCUS       DATRMA010000067        10165 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.067, whole genome shotgun sequence.
ACCESSION   DATRMA010000067 DATRMA010000000
VERSION     DATRMA010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10165
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.067"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            complement(99..1319)
                     /locus_tag="VBC28_001602"
     CDS             complement(99..1319)
                     /locus_tag="VBC28_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HEO6815571.1"
                     /translation="MKNLNRYVVAVSGVVLHLMLGSTYAWSVFRNPIISETGWDISSV
                     SFAFSLAIFCLGMSAAFMGHLVERFGPRIMGMISAILYGAGNVLTGLAIETQQLWLLY
                     VAYGILGGIGLGSGYITPVSTIIKWFPDRRGLATGFAIMGFGFASLVTSPLAQSLMIR
                     IGVGKTFYILGLVYFFVMMIASQFIKQPPQEKITILTHDGKKNAMNSQIITGLKTNAA
                     IKSKTFYIIWLTLFINISCGLGLISAASPMAQDLAGYSAESAALLVGVLGIFNGFGRL
                     LWASLSDYIGRPLTFIILFIVNFIMTSSLFLSFNAIVFAIAMSILMTCYGAGFSLLPA
                     YLSDIFGTKELATLHGYSLTAWAIAGLFGPLLLSKTYSWGNSYQLTLMVFGFLFLFGL
                     LLSLYLRKLTTKVV"
     gene            complement(1506..2873)
                     /locus_tag="VBC28_001603"
     CDS             complement(1506..2873)
                     /locus_tag="VBC28_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEO6815572.1"
                     /translation="MKLSLHEVAKVVGAKNQVSEFEDVPLGNIEFDSRNISEGDLFLP
                     LKGARDGHEFIEMAFDNGAIATISEKEIEGHPYLLVSDALKAFQVLAQYYIEKMNVDV
                     IAVTGSNGKTTTKDMIAAILSTTYKTYKTQGNYNNEIGLPYTVLHMPEDTEKIILEMG
                     QDHLGDIHVLSEIAKPRIAVVTLIGEAHLEFFGSREKIAEGKMQITDGMSSDGILIAP
                     GDPIIDPYLPANQMTIRFGHDQELQVTELKEEKHSLTFKTNALEHQLRIPVPGKYNAT
                     NAMVAAYVGKLLAVAEEDIVDALENLQLTRNRTEWKKSANGADILSDVYNANPTAMRL
                     ILETFSAIPNNDGGKKIALLADMKELGEQSVDLHNQMIMSIRPDSIDTLICYGQDIEG
                     LAQLASQMFPIGKVYFFKKNQEVDQFDQLLAKVKDTLKEKDQILLKGSNSMNLSKIVD
                     ILEVG"
     gene            complement(3020..4066)
                     /locus_tag="VBC28_001604"
     CDS             complement(3020..4066)
                     /locus_tag="VBC28_001604"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEO6815573.1"
                     /translation="MSKETLILLYGGRSAEREVSVLSAESVMRAINYDKFFVKTYFIT
                     QVGQFIKTQEFDEMPSSDEKLMTNQTVDLDKMVRPSDIYDDNAIVFPVLHGPMGEDGS
                     IQGFLEVLRMPYVGTNILSSSVAMDKITTKQVLATVGVPQVAYQTYFEGDDLEHAIKL
                     SLETLSFPIFVKPANMGSSVGISKATDESSLRSAIDLALKYDSRILIEQGVTAREIEV
                     GILGNNDVKTTFPGEVVKDVDFYDYDAKYIDNKITMDIPAKVDEATMEAMRQYASKAF
                     KAIGACGLSRCDFFLTKDGQIFLNELNTMPGFTQWSMYPLLWENMGLTYSDLIEKLVM
                     LAKEMFEKRESHLI"
     gene            complement(4207..4803)
                     /gene="recR"
                     /locus_tag="VBC28_001605"
     CDS             complement(4207..4803)
                     /gene="recR"
                     /locus_tag="VBC28_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HEO6815574.1"
                     /translation="MLYPTPIAKLIDSFSKLPGIGTKTATRLAFYTIGMSDEDVNEFA
                     KNLLAAKRELTYCSVCGNLTDDDPCLICTDKTRDQSVILVVEDSKDVSAMEKIQEYNG
                     LYHVLHGLISPMNGISPDDINLKSLITRLMDGQVTEVIVATNATADGEATSMYISRVL
                     KPAGIKVTRLARGLAVGSDIEYADEVTLLRAIENRTEL"
     gene            complement(4818..6863)
                     /locus_tag="VBC28_001606"
     CDS             complement(4818..6863)
                     /locus_tag="VBC28_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000934659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEO6815575.1"
                     /translation="MLNRKKRYRLTVKKQNASIPRRLNLLFFIIVLLFTVLILRLEQM
                     QIGQQSFYMKKLTALTSYTVKESKARGQIFDAKGVVLVENDERPTVAFSRGNNISSQS
                     IKELANKLSHYITLTEVASSDRAKRDYYLADKANYKKVVESLPDSKRYDKFGNHLAES
                     TVYANAVAAVPVSAINYSEDELKVVALFNQMNATPTFGSVKLSTGELSDDQIKKLDAD
                     KKELLGISVTSNWHRRKKGTSLSDILGTISTEKAGLPREEVKKYLKKGYSLNDRVGTS
                     YLEKQYEDDLQGIRQIRKVVVNKKGKVVSDNITQEGKSGRNLKLTIDLNYQNKVESIL
                     KQYYGSELSSGRASFSEGMYAVAIEPSTGKVLAMAGLKNDHGNLVDDSLGTIAKNFTP
                     GSVVKGATLSSGWENKVLRGNEVLYDQEIANIRSWFTRGLTPISAAQALEYSSNTYMV
                     QVALRLMGQDYNTGDALTDRGYQEAMAKLRKTYGEYGLGVSTGLDLPESEGYVPGKYS
                     LGTTLMESFGQYDAYTPMQLGQYISTIANNGNRLAPHVVSDIYEGNDSNKFAQLVRSI
                     TPKTLNKIAISDQELAIIQEGFYNVVNSGSGYATGTSMRGNVTTISGKTGTAETFAKN
                     VNGQTVSTYNLNAIAYDTNRKIAVAVMYPHVTTDTTKSHQLVARDMIDQYISQFTGQ"
     gene            complement(6992..7684)
                     /locus_tag="VBC28_001607"
     CDS             complement(6992..7684)
                     /locus_tag="VBC28_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018029419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase"
                     /protein_id="HEO6815576.1"
                     /translation="MVKLVFARHGESEWNKANLFTGWADVDLSEKGTQQAIDAGKLIQ
                     AAGIEFDLAFTSVLKRAIKTTNLALEAADQLWVPVEKSWRLNERHYGGLTGKNKAEAA
                     EQFGDEQVHIWRRSYDVLPPDMAKDDEHSAHTDRRYASLDDSVIPDAENLKVTLERAL
                     PFWEDKIAPALKDGKNVFVGAHGNSIRALVKHIKQLSDDEIMDVEIPNFPPLVFEFDE
                     KLNLVSEYYLGK"
     gene            complement(7861..8619)
                     /locus_tag="VBC28_001608"
     CDS             complement(7861..8619)
                     /locus_tag="VBC28_001608"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000087908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEO6815577.1"
                     /translation="MSRKPFIAGNWKMNKNPEEAKAFIEAVASKLPSSELVEAGIAAP
                     ALTLSTVLEAAKGSELKIAAQNSYFENSGAFTGENSPKVLAEMGTDYVVIGHSERRDY
                     FHETDQDINKKAKAIFANGLTPIICCGESLETYEAGKAVEFVGAQVSAALAGLSEEQV
                     SSLVIAYEPIWAIGTGKSATQDDAQNMCKAVRDVVAADFGQAVADKVRVQYGGSVKPE
                     NVAEYMACPDVDGALVGGASLEAESFLALLDFVK"
     gene            complement(8800..9996)
                     /gene="tuf"
                     /locus_tag="VBC28_001609"
     CDS             complement(8800..9996)
                     /gene="tuf"
                     /locus_tag="VBC28_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEO6815578.1"
                     /translation="MAKEKYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLARRLPTSV
                     NQPKDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQM
                     DGAILVVASTDGPMPQTREHILLSRQVGVKHLIVFMNKVDLVDDEELLELVEMEIRDL
                     LSEYDFPGDDLPVIQGSALKALEGDEKYEDIIMELMSTVDEYIPEPERDTDKPLLLPV
                     EDVFSITGRGTVASGRIDRGTVRVNDEVEIVGIKEDIQKAVVTGVEMFRKQLDEGLAG
                     DNVGVLLRGVQRDEIERGQVLAKPGSINPHTKFKGEVYILSKEEGGRHTPFFNNYRPQ
                     FYFRTTDVTGSIELPAGTEMVMPGDNVTIEVELIHPIAVEQGTTFSIREGGRTVGSGI
                     VSEIEA"
BASE COUNT         3183 a         2034 c         1629 g         3319 t
ORIGIN      
        1 tagatactta gtatatcaaa ttatttgcta taagacaaaa ctatttttca aagaaaaaac
       61 tctttggtaa aaccaaagaa ttttaaatta gatatatact acacaacttt agttgttaat
      121 tttcttaaat atagagataa caataatccg aataagaata aaaaaccaaa aaccattaat
      181 gtcaattgat aggaatttcc ccatgaatat gtctttgata acaatagggg cccaaacaga
      241 cctgctattg cccatgctgt taaactataa ccatgtaaag tagctaattc ctttgttcca
      301 aaaatatcac ttagataagc aggtaataag gaaaagcctg caccataaca agtcattaaa
      361 atagacattg ctattgcaaa tacaatagca ttgaatgaca aaaataaact agaagtcata
      421 ataaagttca caataaataa tattataaag gtcaacgggc gtccaatgta gtcagagaga
      481 cttgcccata acagacgtcc aaaaccgtta aatatcccta gtacccctac taataaggct
      541 gcagattctg cggaatagcc tgctaaatct tgtgccattg gtgaagctgc tgatattaaa
      601 cctaagccac acgaaatatt aataaacaaa gtcaaccaaa tgatgtaaaa ggtttttgat
      661 tttatagcag catttgtttt taatccagta ataatttgtg aattcatagc attcttttta
      721 ccgtcgtgag tcaaaatagt tattttttcc tgaggtggtt gtttaataaa ttgtgaggca
      781 atcatcatga caaaaaaata tactaatccc aaaatataaa atgttttacc tacaccaatc
      841 ctaatcatta aggattgtgc aagcggactt gttactaaag aagcaaagcc aaatcccata
      901 atagcgaatc ctgttgctag tcccctccta tcaggaaacc atttaataat agtcgatact
      961 ggagtaatat aacctgaacc aagtccgatt cctcctaaaa taccgtatgc aacatacagt
     1021 aaccataact gctgagtttc aatggctaaa cctgttaaca cattccctgc tccatataaa
     1081 atagcagaaa tcattcccat tatcctagga ccaaaacgct ctactaagtg tcccataaaa
     1141 gctgcagaca ttcctagaca aaaaatagcc aaactaaaag cgaatgaaac tgatgaaata
     1201 tcccaaccag tctctgagat gattgggtta cgaaacacac tccaagcata agttgatcct
     1261 agcattaaat gtaaaacgac tccagaaacc gcaactacat aacgatttaa atttttcaaa
     1321 caatcctcct aaaaacgaac ttaagtttta tttttaaaaa tatagttcgt aataaacaga
     1381 ttatcaaaaa actgaatata tcaccagttt tctgcttagc atatctattt tcctcaataa
     1441 aactgaatat tattcgtgat taaaaaacaa aaaagttagg gtaacttgtt accctaacac
     1501 ctctattatc ctacttcaag aatatcaact attttagaaa gattcattga gttagaacct
     1561 tttaataata tttgatcttt ctccttaaga gtatctttta cttttgcaag caattgatca
     1621 aactgatcta cttcttgatt ctttttaaag aaatacactt taccaattgg gaacatctgg
     1681 cttgctaatt gagccaaccc ctcaatatcc tggccataac aaatcaaagt atcaatagag
     1741 tcaggtctga tactcataat catttgatta tgtaaatcca cagattgttc tcccagttct
     1801 ttcatatcag ctaaaagagc aatcttcttt ccaccatcat tattgggaat agctgaaaag
     1861 gtttctagta tcaatcgcat agccgttggg ttagcattgt aaacatctga taaaatatct
     1921 gctccattgg ctgatttctt ccattcggtg cgattacgag ttagttgaag attttctaga
     1981 gcatccacta tatcttcttc tgctactgct aataattttc caacataggc agcgaccatc
     2041 gcattagttg cattgtactt accaggtact ggtatacgta attggtgctc caaagcatta
     2101 gttttaaaag ttaaggaatg tttttcttct ttgagttctg tcacttgcaa ttcttggtca
     2161 tgaccaaaac gaatagtcat ctggtttgct ggaagatatg gatcaataat tggatctccc
     2221 ggtgcaatta atatgccatc tgaactcata ccatctgtga tttgcatttt cccttctgca
     2281 atcttttctc gactaccaaa aaattctaag tgtgcttcac caatcaaagt aacaacagca
     2341 atacgtggtt ttgcaatttc agataacaca tggatatctc caagatgatc ctgtcccatc
     2401 tccaaaataa ttttttcagt atcttctggc atgtgtagca cagtataggg taagccaatc
     2461 tcgttattgt agtttccttg tgttttataa gtcttatatg tggttgataa gattgcagct
     2521 atcatatctt tagttgttgt cttaccattt gagccagtta ctgcaataac atcaacgttc
     2581 atcttttcaa tataatactg tgccaagact tgaaatgctt taagtgcatc agacactaaa
     2641 agataaggat gaccctctat ttccttctct gaaatggttg ctattgctcc attatcaaaa
     2701 gccatctcta taaactcatg tccatctctt gctcctttta agggaagaaa taagtcacct
     2761 tcactgatat tacgactatc aaattcaata tttcccaggg gaacgtcttc aaactctgat
     2821 acttgatttt tagcgccaac tactttagca acttcatgca aactaagctt catatcattt
     2881 ccttttctat tatttagcaa caatgttgta gatagtatgc tatcccttat aaacgaatat
     2941 actcctaaat tataacacac tttcataaca tatcatagat ttgaaaaagc tcaactaaga
     3001 aagacttagt tgaacttttt taaattaaat gactctcacg tttttcaaac atttctttag
     3061 ctaacataac taacttttca atcaaatcac tataagttag ccccatattt tcccaaagca
     3121 ggggatacat tgaccactga gtaaaaccgg gcattgtatt cagttcgttt aagaagattt
     3181 gtccatcttt cgtcaaaaag aaatcacagc gtgataaacc acaagccccg attgctttaa
     3241 aagctttact tgcatattga cgcattgctt ccatagttgc ttcatcaact ttagctggaa
     3301 tatccatagt aattttatta tcaatatatt tggcgtcata gtcatagaaa tcgacgtctt
     3361 taacgacttc gccaggaaaa gttgtcttaa catcattatt gcctaaaata cctacttcaa
     3421 tttcacgagc tgtcacgcct tgttcaatca aaatacggct atcatacttg agagctaagt
     3481 caattgcaga gcgaagtgag gattcatctg tcgcttttga aatacctact gatgacccca
     3541 tattagccgg ttttacaaaa attgggaaac ttaaagtttc taaagagagt ttaatcgcat
     3601 gttccaaatc atcaccctca aaataagttt gatatgcaac ctgaggtaca cctactgttg
     3661 caaggacttg ttttgttgta attttatcca tagccacgct tgaagataga atattagtcc
     3721 caacataagg catccttaaa acttctaaaa atccttggat agaaccatct tcccccattg
     3781 gtccatgtaa aacggggaaa acaattgcat tatcatcata gatatcactt ggacgaacca
     3841 ttttgtctaa atcaacagtt tggtttgtca ttaacttttc atctgaagat ggcatttcat
     3901 caaattcttg tgttttaata aattgaccta cttgcgtgat aaaataagtt ttaacaaaaa
     3961 atttatcata attaatagca cgcatgacgc tttctgcaga cagtacagat acttcacgtt
     4021 ccgcagagcg accaccgtaa agcaagatca atgtttcttt tgacatattt cttctctttt
     4081 ctatctaatc taagattgac cttttaataa taccatattt taacctaact ttcaaaaatc
     4141 aatccactta aagttagtat tgtaaaagaa aaagttagga gattcccaac cttttcttta
     4201 taacaattat aactctgtac gattttcgat tgctcgaagc aaggttactt catctgcata
     4261 ttcgatatct gaaccaacag ctaaacctct cgctaatcgt gtcactttga taccagctgg
     4321 ttttaagact cgtgaaatat acattgatgt agcttcacca tcagcagtcg cattggtagc
     4381 aacaataact tctgtcacct gaccatccat caatcgagta attaaacttt tcaaattaat
     4441 gtcatcaggg ctaataccat tcataggtga aattaagcca tgtaagacat gatacaaacc
     4501 attatactct tgaatttttt ccatagctga cacatcttta ctatcttcaa caaccaagat
     4561 aacagactgg tcacgcgtct tatctgtaca aattaagcaa ggatcatcat cagtcaaatt
     4621 accacaaaca gaacaatagg ttaattcccg cttagcagct aataaattct tagcaaattc
     4681 attaacatct tcatcactca taccaatggt gtaaaaagct aaacgtgttg ctgttttagt
     4741 acctataccc ggtaattttg aaaagctgtc aatcaattta gcaattggtg ttgggtaaag
     4801 cattcaaatg tcctctctta ttgtcctgtg aactgtgaaa tatattgatc aatcatatct
     4861 cgtgcaacta attgatggga ttttgttgta tcagttgtaa catgcggata cattaccgct
     4921 actgctattt tacgattagt atcgtaggca atagcgttta agttgtaggt agaaactgtt
     4981 tgtccattta catttttagc aaatgtttca gcggtaccag ttttaccact aatggttgtc
     5041 acattccccc tcattgacgt accagttgca tagccacttc cactattgac aacgttataa
     5101 aaaccttctt gaataatggc taactcttga tctgagatag ctatcttatt tagtgtttta
     5161 ggagtgattg aacgaaccaa ttgagcgaac ttattagaat cattcccttc atagatatct
     5221 gaaaccacgt gaggtgctaa acgattccca ttattcgcaa tagttgagat atactgacca
     5281 agttgcattg gtgtataggc atcatactga ccgaacgatt ccattagagt tgttcctaag
     5341 ctgtattttc caggtacata accttctgat tcaggtaaat ctaatcctgt agaaacccct
     5401 aaaccatatt cgccgtaagt tttccttagt ttagccattg cttcttgata gcctcgatca
     5461 gttaaagcat ctcctgtatt atagtcttgt cccattaaac gaagtgctac ttgaaccata
     5521 taagtattag atgaatactc tagcgcttgt gccgcagata ttggagtcaa acctcgtgta
     5581 aaccatgacc gtatattagc aatttcttgg tcataaagca cttcatttcc tctaagaact
     5641 ttattttccc atcctgatga tagcgtcgca cctttcacaa cagatcctgg cgtgaaattc
     5701 ttagcaatag tgcctaggct gtcatcaact aaattcccat gatcattttt cagaccagcc
     5761 attgctaaca ctttaccagt tgaaggttct atagcaactg catacatccc ttcggaaaaa
     5821 ctagcgcgtc cactagataa ttcactgcca tagtattgtt tcaagatact ttctactttg
     5881 ttttggtaat taagatcaat cgttaattta agatttcttc ctgacttacc ttcttgcgta
     5941 atattatctg aaacaacttt acctttttta ttaacaacta ctttgcgaat ttgtctgatt
     6001 ccttgtaagt catcttcata ttgtttttca aggtaagaag ttcccactcg gtcattcagt
     6061 gaatagcctt ttttcaaata ttttttaact tcttctcttg gtaaacctgc cttttctgta
     6121 gaaatagtac ctaatatatc cgataaagaa gttccttttt tacgacgatg ccaattactt
     6181 gttacagaaa ttcctaacag ttccttctta tcagcatcca gtttcttgat ttgatcatca
     6241 cttaattcgc ctgtacttaa tttcactgaa ccaaacgttg gtgtcgcatt catttggtta
     6301 aataaggcaa ccacttttaa ctcatcttca gaatagttaa tagcgctcac tggaactgca
     6361 gcaactgcat ttgcatatac ggttgattcg gctagatggt taccaaattt atcgtaacgt
     6421 ttagaatctg gtaaactttc aacaactttt ttgtagttgg ctttatctgc taaataataa
     6481 tctctctttg ctctatcgct tgaagcaact tcggttaagg taatgtaatg tgataattta
     6541 ttcgctaact ctttgataga ctgagatgaa atattgtttc ctctagaaaa cgccacagta
     6601 ggacgttcat cattttcaac taagacaact cccttagcat caaaaatctg acctctcgct
     6661 tttgattctt tcactgtata actcgtaaga gctgtcagtt ttttcatgta aaatgattgt
     6721 tgcccaatct gcatttgctc taaacgaaga attagaacag taaataataa aacaataata
     6781 aaaaatagaa gatttaatcg tctaggtatg ctagcatttt gcttttttac agtgagacga
     6841 tacctttttt tacgattcaa cattaactcc ttgctataaa aactctgact actattgtat
     6901 cataaaaatg gttttttaca gacactcaaa atgttataaa taataaaaaa ttcaagccaa
     6961 cttggcctga attttttata taaagattag attatttacc taagtaatat tctgaaacaa
     7021 ggtttaattt ttcatcaaat tcgaaaacaa gtggtgggaa gttaggaatt tcaacgtcca
     7081 tgatttcatc atctgacaat tgtttgatat gttttacaag agcacggatt gagttaccgt
     7141 gtgcaccaac aaacacattt ttaccatctt taagagcagg agcaatttta tcttcccaga
     7201 aaggaagagc acgctctaaa gtaactttta ggttttctgc atctggaata acagaatcat
     7261 ctagtgaagc atagcgacga tcagtatgtg ctgaatgttc atcatcttta gccatatctg
     7321 gaggcaatac atcatatgaa cgacgccaaa tatgaacttg ctcatcacca aattgttcag
     7381 ctgcttctgc tttatttttt cctgtcaatc caccgtaatg acgttcgttc aagcgccatg
     7441 atttttcaac tggtacccaa agttgatcag ctgcttcaag ggcaaggtta gttgttttga
     7501 tggcacgttt aagaactgat gtaaaagcaa ggtcgaactc aatacctgct gcttgaatta
     7561 atttcccagc atcaatagct tgttgtgtac ctttttctga aagatctacg tcagcccatc
     7621 cagtgaaaag gttagcttta ttccactcag attcaccgtg gcgtgcgaat actaatttta
     7681 ccattagaaa atttctcctt taattttggc tataccaata tttactttaa ctattctaca
     7741 aaaaatagcc ataaaaatct agtctgatta ctagttcaat aatcaaaaat agcttattat
     7801 taactatcta taagaaaagg catatcacct agagtgctat gcctttagat atcatgtgat
     7861 ttatttaaca aaatctaata aggcaaggaa gctttctgct tcaagtgaag ctccacctac
     7921 aagggcaccg tcaacgtctg ggcaagccat atattctgca acgttttcag gtttaactga
     7981 accaccgtat tgaacgcgaa ctttgtcagc aactgcttga ccgaagtctg ctgcaacaac
     8041 gtcacgaact gctttacaca tgttttgtgc gtcgtcttga gttgctgatt taccagtacc
     8101 aatagcccag attggttcgt aagcgataac aagtgatgat acttgctctt ctgacaaacc
     8161 tgcaagagct gctgaaactt gagcacctac gaattcaacc gctttaccag cttcgtaagt
     8221 ttcaagagac tcaccgcaac aaatgattgg agtcaaacca ttagcaaaga tagcttttgc
     8281 ttttttgttg atatcttggt cagtttcatg gaaataatca cgacgttctg agtgaccgat
     8341 aacaacgtag tctgtaccca tttcagccaa tactttaggg ctgttttcac cagtgaatgc
     8401 acctgaattc tcaaaatagc tattttgagc agcgattttc aattctgaac ctttagctgc
     8461 ttcaagaact gttgataaag taagcgctgg agcagcaatc cctgcttcaa caagttctga
     8521 tgatggtaat ttagaagcta ctgcttcgat gaatgctttt gcttcttctg gatttttatt
     8581 cattttccag ttaccagcga taaatggttt acgtgacatt acacatacct cttctttttt
     8641 tatttactaa tctatcatac cataaattat agttaattaa aagtttcacg agtctagtaa
     8701 aacgctttaa ttatagatac taaaaaagag tccctgagga ctcttcacaa tttgagctgt
     8761 ctgactctaa ctgccgtttg ggaacttaat taagttaaat taagcttcga tttctgaaac
     8821 gatacctgaa ccaacagtac gtccaccttc acggattgag aaagtagtac cttgttctac
     8881 ggcgattggg tgaatcaatt caacttcgat agtaacgtta tcaccaggca taaccatttc
     8941 tgttcctgct ggaagttcga ttgaacctgt tacgtcagtt gtacggaagt agaattgtgg
     9001 acggtagttg ttgaagaatg gagtatgacg tccaccttct tctttagaaa ggatgtaaac
     9061 ttcaccttta aatttagtgt gtgggttgat tgaacctggt ttagcaagaa cttgaccacg
     9121 ttcgatttca tcacgttgaa caccacgaag aagaacacca acgttgtccc ctgcaagacc
     9181 ttcgtcaagt tgtttacgga acatttcaac accagtaaca actgcttttt ggatatcttc
     9241 tttaatacca acgatttcaa cttcgtcgtt gacacgaaca gtaccacggt cgatacgtcc
     9301 tgaagcaact gtaccacgtc cagtgattga gaatacatct tcaactggaa gaagtaaagg
     9361 tttgtcagta tcacgttctg gttctggaat gtactcatca acagtgctca tcaattccat
     9421 gatgatgtct tcgtattttt cgtcgccttc aagtgcttta agagctgaac cttggataac
     9481 tggaaggtca tcacctggga agtcgtattc tgaaagaagg tcacgaattt ccatttcaac
     9541 caattcaagc aattcttcat catcaacaag gtcaactttg ttcatgaata cgataaggtg
     9601 tttaacacca acttgacgtg aaagaaggat gtgctcacga gtttgtggca ttggtccatc
     9661 agttgaagca actacaagga tagctccgtc catttgagca gcaccagtga tcatgttttt
     9721 aacgtagtcc gcgtgtcctg gagcgtcgat gtgggcatag tgacgttttt cagtttcgta
     9781 ctcaacgtgt gcagtgttga ttgtgattcc gcgttcgcgc tcttctggag cagcatcgat
     9841 agaagcgtaa tcttttggtt ggttaactga agtaggcaag cgacgtgcca atacagttgt
     9901 gatagctgct gttaaagtag ttttaccgtg gtcaacgtgt ccgattgtac caatgttaac
     9961 gtgtggttta ctacgatcgt atttttcttt tgccatttta ggaaaagcct ccaataaaat
    10021 atattttata gatagacggt aggcaataca gtctaacttt acttatctat tttaacaaaa
    10081 tcctctaaaa aagcaagtat tttatgcttt tttgcttatt tctttatttc cttaaaattc
    10141 gggtaagatt gattgaggca tttgt
//
LOCUS       DATRMA010000068        10058 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.068, whole genome shotgun sequence.
ACCESSION   DATRMA010000068 DATRMA010000000
VERSION     DATRMA010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10058
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.068"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            complement(<1..115)
                     /locus_tag="VBC28_001610"
     CDS             complement(<1..115)
                     /locus_tag="VBC28_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001931829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEO6815579.1"
                     /translation="MVKRDFIRNIILALIAVLILILLRYFVFATFKVHKDAT"
     gene            complement(286..1788)
                     /gene="pyk"
                     /locus_tag="VBC28_001611"
     CDS             complement(286..1788)
                     /gene="pyk"
                     /locus_tag="VBC28_001611"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018366040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEO6815580.1"
                     /translation="MNKRVKIVATLGPAVEFRGGKKFGESGYWGESLDVEASAEKIAQ
                     LIKEGANVFRFNFSHGDHAEQGARMATVRKAEEIAGQKVGFLLDTKGPEIRTELFEDG
                     ADFHSYTTGTKLRVATKQGIKSTPEVIALNVAGGLDIFDDVEVGKQILVDDGKLGLTV
                     FAKDKDTREFEVVVENDGLIGKQKGVNIPYTKIPFPALAERDNADIRFGLEQGLNFIA
                     ISFVRTAKDVNEVRAICEETGNGHVKLFAKIENQQGIDNIDEIIEAADGIMIARGDMG
                     IEVPFEMVPVYQKMIITKVNAAGKAVITATNMLETMTDKPRATRSEVSDVFNAVIDGT
                     DATMLSGESANGKYPVESVRTMATIDKNAQTLLNEYGRLDSSAFPRNNKTDVIASAVK
                     DATHSMDIKLVVTITETGNTARAISKFRPDADILAVTFDEKVQRSLMINWGVIPVLAD
                     KPASTDDMFEVAERVALEAGFVESGDNIVIVAGVPVGTGGTNTMRVRTVK"
     gene            complement(1837..2859)
                     /gene="pfkA"
                     /locus_tag="VBC28_001612"
     CDS             complement(1837..2859)
                     /gene="pfkA"
                     /locus_tag="VBC28_001612"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEO6815581.1"
                     /translation="MKRIAVLTSGGDAPGMNAAIRAVVRKAISEGMEVYGINQGYYGM
                     VTGDIFPLDANSVGDTINRGGTFLRSARYPEFAELEGQLKGIEQLKKHGIEGVVVIGG
                     DGSYHGAMRLTEHGFPAVGLPGTIDNDIVGTDYTIGFDTAVATAVENLDRLRDTSASH
                     NRTFVVEVMGRNAGDIALWSGIAAGADQIIVPEEEFNIDEVVSNVRAGYAAGKHHQII
                     VLAEGVMSGDEFAKTMKAAGDDSDLRVTNLGHLLRGGSPTARDRVLASRMGAYAVQLL
                     KEGRGGLAVGVHNEEMVESPILGLAEEGALFSLTDEGKIVVNNPHKADLRLAALNRDL
                     ANQSSK"
     gene            complement(2940..6044)
                     /locus_tag="VBC28_001613"
     CDS             complement(2940..6044)
                     /locus_tag="VBC28_001613"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEO6815582.1"
                     /translation="MFAQLDTRTVYSFMDSLVDLKTYVSKSKSLGYQTIGILDHSNLY
                     AAYHFIQEAQKANLRPIVGFSFDIVVENRSIEVYCIAINTVGYKNLLKLSTAQMSEKM
                     SLNLLTEHLEGVQLILPYQDVLDQLNLPFDYVIGVNLTSPQIPYTKPIIAIDTVRYFQ
                     KNDIETLQMLHAIRDNVLLKDAQYASKNQELKPCQEMTLAFKERFPEALANLESLVEN
                     VAYHFDSDFKLPIFNREIPAGEELRTLTQNNLKSKGLWSDAYQERLEKELTIIHKMGF
                     DDYFLIVWDLLRFGRSKGYYMGMGRGSAAGSLVSYALNITGIDPVKHNLIFERFLNEE
                     RYSMPDIDIDLPDIYRGEFLRYVRNRYGSMHSAQIVTFSTFGAKQAIRDVFKRFGASE
                     YELTNITKKIHFRDNLTSVYNRNLAFRQIIDSKIEYQKAYDIAKRIEGNPRQTSIHAA
                     GVVMSDDLLTDHIPLKNGEDMMITQYDASSVEDNGLLKMDFLGLRNLTFVQKMKEKVD
                     KDYGISIQLETIDLEDKETLKLFAAGQTKGIFQFEQSGAINLLRRIRPECFEDVVATT
                     SLNRPGASDYTENFINRRFGKEKIDLVDPVIAPILQPTYGIMLYQEQVMQIAQTYAGF
                     TLGKSDLLRRAMSKKNSKEMQKMSQSFLEGAVSKGHRQEDARLIFERMAKFAGYGFNR
                     SHAFAYSALAFQLAYFKAHYSDVFYDIMINYSNSDYLIDAIDFGFVIEKPSINTISYR
                     DRIYKKKIYLGLKNIKGVPNDLAYWISKNQPFQSIEDFLMRLPQQFQKSGFISPLIAI
                     GAFDEFDNNRRKITSNLDSLFTFVNELGSLFADTSYHWLEVEDFSNSEKYEMEQDILG
                     VGISPHPLVGISQKASRPFIPISQVQENSEATILVQLKQVKVIRTKSSGQQMAFLTVM
                     DINSKMDITVFPETFNIVRDDLQEGKYYYLHGKIQKRDERLQMVLNGVQEATEERFWI
                     LLKNHDNDKKISEILSKYKGHIPVYLHYETTKETIQSKVHLVRKDSGLALDLSEFVVK
                     TVYQ"
     gene            6189..6557
                     /locus_tag="VBC28_001614"
     CDS             6189..6557
                     /locus_tag="VBC28_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEO6815583.1"
                     /translation="MAWEFNEKSPIYSQIAEHIKMQIVSQEIKSGDQLPTVRELAQEA
                     GVNPNTMQRAFTELEREGMVFSQRTSGRFVTEDNLLIGKIRQQVAKAELATFVNNMKK
                     IGYKLDEITVALDHFIKEIN"
     gene            6558..7256
                     /locus_tag="VBC28_001615"
     CDS             6558..7256
                     /locus_tag="VBC28_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6815584.1"
                     /translation="MTQLLQLHHVTKKYHKHTAVNDVTVSIPTGKIIGLLGPNGSGKT
                     TIIKMINGLLQPDKGDIVIDGYRPSVDTKKIISYLPDTSYLQENMKIKDVVTLFEDFY
                     NDFDSKVAYQLFEDLNLNPRERLKNLSKGNKEKVQLILVMSRKARLYILDEPIGGVDP
                     AARDYILKTIISNYSNDASVLISTHLISDIEPILDEVIFLKEGEIDLQGNADDLREEH
                     NCSIDALFRERFKK"
     gene            7268..8050
                     /locus_tag="VBC28_001616"
     CDS             7268..8050
                     /locus_tag="VBC28_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter (permease)"
                     /protein_id="HEO6815585.1"
                     /translation="MFGKLLKYELKSVGKWYLTLNAAVLLVSIILGLVLKALGGNFST
                     DTNSTSAQIFTIILVLLLAMVISGSLLSTLAIIIKRFYSNIFGRQGYLTLTLPVTTNQ
                     IICSKLLASLLWSIFNIFIVIIGIILVILPLVGIGQFVVAFPEIYKIISSSNAPLFIA
                     YFFLSYVAGTLLIYLSIAVGQLFTNKRVLMGIVSYFGISLLITFLTLIIDSIFHIDLF
                     NSHANATFSQPVLLYNILVSIVEIAIFYMLTHSIIKYKLNIQ"
     gene            complement(8087..8698)
                     /locus_tag="VBC28_001617"
     CDS             complement(8087..8698)
                     /locus_tag="VBC28_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001056395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEO6815586.1"
                     /translation="MNMKLSKRYRFWQKVIKALGVLALIATLVLVVYLYKLGILNDSN
                     ELKDLVHKYEFWGPMIFIVAQIVQIVFPVIPGGVTTVAGFLIFGPTLGFIYNYIGIII
                     GSVILFWLVKFYGRKFVLLFMDQKTFDKYESKLETSGYEKFFIFCMASPISPADIMVM
                     ITGLSNMSIKRFVTIIMITKPISIIGYSYLWIYGGDILKNFLN"
     gene            8791..9140
                     /gene="ssrA"
                     /locus_tag="VBC28_001618"
     tmRNA           8791..9140
                     /gene="ssrA"
                     /locus_tag="VBC28_001618"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            9409..10047
                     /locus_tag="VBC28_001619"
     CDS             9409..10047
                     /locus_tag="VBC28_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HEO6815587.1"
                     /translation="MASILKKHLEKRLELKNDPMRTDMSTGASYPKYGFSVGKYTYGY
                     QQFFYEGVNLKEIGAFCSIAQNVTITGLNHPTDHITTNPFIYYKSRGFINEDRADLID
                     EKKNGKVIIGNDVWIGTNVTILPSVTIGNGAIIGAGSVITKDIPDYAVVAGTPAKIIK
                     YRFSEEEITLLNASQWWNWSDEAIKEHISEFSDKKEFFNTLKSISENKNHKL"
BASE COUNT         3307 a         1924 c         1590 g         3237 t
ORIGIN      
        1 ttgtagcatc cttatgtact ttaaaggttg caaaaacaaa atatctcaag agaatcaaaa
       61 tcaatacagc aatcaatgct aaaataatat ttctaataaa atcgcgttta accataacta
      121 ctcctaatct tttttccatt atatcatgtt ttcatagata aaaaaaggag ggtaattatt
      181 aatatcatat cctattctag tcacaataat cgaataataa aaagcaaaac atataaaaaa
      241 cccattattt aaggtattct taaataatag gtttgaattc aaaaattatt taacagtacg
      301 aacacgcatt gtgttagttc cacctgtacc tacaggaaca cctgcaacga taacgatatt
      361 atcgcctgat tcaacaaatc ctgcttcaag tgctacacgt tctgcaacct caaacatatc
      421 atctgtagat gctggtttgt ctgcaaggac agggataaca ccccagttaa tcatcaatga
      481 acgttgtact ttttcatcaa atgtaacagc caaaatgtct gcatctggac ggaatttaga
      541 aatggcacga gctgtattac ctgtttcagt aattgttaca acaagtttga tatccattga
      601 gtgtgttgca tctttaaccg cagatgcaat aacatcagtt ttgttattac gtgggaatgc
      661 agatgagtct aagcgaccat actcattgag taatgtttga gcatttttat caatagtagc
      721 cattgtacga actgactcaa ctgggtattt accattagct gactcacctg aaagcattgt
      781 agcatcagta ccatcaataa cagcattgaa gacatcagat acttctgaac gagtcgcacg
      841 tggtttatca gtcattgttt caagcatatt tgttgctgta ataactgctt taccagctgc
      901 attaacttta gtaatgatca ttttttggta aactggaacc atttcaaatg gaacttcgat
      961 acccatatca ccacgagcaa tcataatacc atctgctgct tcgataatct catcaatatt
     1021 atcgatacct tgttgatttt caattttagc aaacaactta acgtgtccat tgccagtttc
     1081 ttcacaaata gcacgaactt cattaacatc tttagcagta cgtacaaatg agatagcaat
     1141 aaagttaagt ccttgctcaa gtccaaaacg gatatcagca ttatcgcgtt ctgcaagtgc
     1201 tgggaaagga attttagtat aagggatgtt tacacctttt tgtttaccaa taaggccatc
     1261 attctcaaca actacttcaa attcacgagt gtctttatct tttgcaaaca cagtaagacc
     1321 tagtttacca tcatcaacaa ggatttgctt accaacttca acgtcatcaa agatgtcaag
     1381 tccaccagca acattcaatg caatcacttc tggagttgat ttgatacctt gcttagtagc
     1441 aacacgtaat tttgtacctg ttgtatatga atggaaatct gcaccatctt caaaaagttc
     1501 tgtacgaatt tcaggtcctt tagtatcaag gaggaagcca actttttgtc ctgcaatctc
     1561 ttctgcttta cgaacagtag ccatacgagc tccttgctca gcatgatctc catgtgagaa
     1621 gttgaaacgg aaaacgttag caccttcttt aatcaattga gcaatttttt ctgctgaagc
     1681 ttctacgtca aggctttcac cccagtatcc agactcacca aacttcttac caccacggaa
     1741 ttcaaccgca ggaccaagtg ttgcaacgat ttttacgcgt ttattcataa taatttatga
     1801 gctccttttt atttaaagtg tcaaattgac aaattcttat ttactacttt ggttggcaag
     1861 gtcacgatta agtgctgcca agcgaaggtc cgctttatgc ggattattaa caacgatttt
     1921 tccttcatca gtcaagctga acaaagcacc ttcttctgct aaacctaaaa ttggactttc
     1981 aaccatttct tcgttgtgga caccaacggc taaaccacca cgaccttctt tcaacaattg
     2041 aacagcgtac gctcccatac gagatgctaa gacacgatca cgagccgtcg gactaccacc
     2101 acggagcaga tgtcctaaat tcgtcacacg aagatcgcta tcgtctcctg ctgctttcat
     2161 tgtttttgca aactcatcac cactcataac accttctgca aggacgatga tttggtgatg
     2221 tttaccagct gcatagccag ctctaacatt tgagacaact tcatcaatat tgaactcttc
     2281 ttcaggaaca ataatttgat ctgcacctgc agcgatacct gaccaaagag cgatatctcc
     2341 tgcatttctt cccataacct caacaacaaa agtacggtta tgacttgctg atgtatcacg
     2401 aagacggtca agattctcaa ctgctgtcgc aactgctgtg tcaaaaccaa tagtatagtc
     2461 agtgccaacg atatcgttat caattgtacc cggcaaacca acagctggga aaccgtgctc
     2521 agttagacgc atagcaccat gataagaacc atcaccaccg ataactacta caccttcaat
     2581 accgtgtttt ttaagctgtt caatcccttt aagctgacct tcaagttcag caaattcagg
     2641 ataacgtgct gaacgtaaaa acgttcctcc acggttgata gtatccccaa cagaattagc
     2701 atccaaaggg aaaatatccc ctgtcaccat accatagtaa ccttggttga tgccgtaaac
     2761 ttccatacct tcagaaattg ctttacgaac aactgcacgg atagcagcgt tcataccagg
     2821 ggcgtcacca ccactagtta aaacagcaat acgtttcatt taattggtac tccttttatt
     2881 aatttaacga tttaattata gcatattaat ttgtaaattt ctgcacttta tatgcaattt
     2941 tattgataaa ccgttttcac aacaaattca gataaatcaa gggctaagcc actatctttt
     3001 ctaaccaaat gtactttgct ttggatagtt tctttagttg tttcataatg caaatagaca
     3061 ggaatatgcc ctttgtattt ggacaatatt tccgatattt ttttgtcgtt atcatgattt
     3121 tttagaagta tccaaaaacg ttcctccgtt gcttcttgaa caccgtttaa aaccatctgt
     3181 aatcgctcat ctcgcttctg aatttttcca tgcaagtaat aatatttccc ttcttgtaaa
     3241 tcatctctta caatattgaa agtttcagga aaaacagtaa tatccatttt tgaattaata
     3301 tccataacag tcaagaaagc catttgttgc ccagaacttt ttgtcctaat aactttgacc
     3361 tgctttagct gaactaaaat agtagcttcg ctattttctt gaacttgact aattggtata
     3421 aacggacggc tagctttttg tgaaatcccc actaaagggt gcgggctgat tcctactcct
     3481 aagatatctt gttccatttc atatttctca gaattagaga aatcttctac ttctagccaa
     3541 tgatatgagg tatctgcaaa taaactacct agttcattta caaatgtaaa taaagaatca
     3601 agattagagg tgattttccg tctattatta tcaaattcgt caaatgctcc aatagcaatt
     3661 agcggagaaa taaaaccact tttttgaaac tgctgtggca gcctcattag aaaatcttca
     3721 atactctgaa acggttgatt tttagaaatc caataagcca aatcgttagg aacaccctta
     3781 atatttttta aacctaaata aattttcttt ttatagatgc ggtctctgta tgaaatagta
     3841 ttaattgaag gtttttcaat aacaaaacca aaatcaatag catcgattag ataatcacta
     3901 tttgaataat ttatcatgat gtcataaaag acatcggaat aatgggcttt gaaataggct
     3961 aattgaaaag ctaaagccga ataagcaaac gcatggctgc gattaaaccc ataacctgca
     4021 aatttagcca tgcgttcaaa tataagtcga gcatcttctt gacgatgtcc cttagatact
     4081 gcaccctcta agaaggattg agacatcttt tgcatctcct tagaattttt cttagacata
     4141 gcacgtctga gcaaatcaga cttgcctagc gtaaaacctg cgtaggtctg ggcaatctgc
     4201 ataacctgtt cttgatagag cattatgcca taggtaggtt gtaaaattgg agctatgaca
     4261 ggatctacta aatcgatttt ttctttacca aaacgacggt ttataaaatt ctcagtataa
     4321 tcacttgcac caggcctatt caagctggtg gtagctacca catcttcaaa acactctggc
     4381 ctaatacgtc gcaacaaatt aatagctcca ctttgctcaa attgaaaaat cccctttgtt
     4441 tgaccagctg caaatagttt tagagtctct ttatcttcta aatcaatagt ttctaattgt
     4501 atagagatac cgtagtcctt atcgactttc tccttcattt tctgaacaaa tgtcaaattt
     4561 cgaagaccta gaaagtccat ttttagaaga ccattatcct caacagaact agcatcatat
     4621 tgcgttatca tcatatcctc accgtttttt aaaggaatat gatctgttaa caaatcgtca
     4681 ctcattacta ccccagcggc atgaatagaa gtctgtctag gatttccttc aatacgctta
     4741 gcaatatcat aggctttttg gtattcaatt ttactatcta ttatttgtct gaaagctaag
     4801 ttgcggttat aaacactggt caaattatct ctgaaatgaa tttttttagt tatgtttgtt
     4861 aactcatact ctgatgcacc aaagcgttta aaaacatctc gaatagcctg tttcgcacca
     4921 aaagttgaga aggttacaat ctgtgcagaa tgcattgaac catatcgatt acgtacatag
     4981 cgcaagaatt ctccacgata aatatccgga agatcaatat caatatctgg catcgaataa
     5041 cgctcttcat ttaaaaaacg ttcaaaaatt aaattatgtt tgacaggatc gataccggta
     5101 atatttaaag catatgagac taaacttccc gctgctgaac cacgtcccat tcccatatag
     5161 tagcccttac tacgaccaaa tcttaataaa tcccacacaa taaggaaata gtcatcaaag
     5221 cccattttgt gaatgattgt taattctttt tcgagacgct cttgataagc atcgctccat
     5281 aaacctttac tttttaagtt attttgagtt aaggttctta attcttctcc agcaggaatt
     5341 tctctattaa aaataggtaa cttaaagtca gaatcaaagt gataagcaac attctctacc
     5401 aaagattcta ggttagctaa agcttcagga aaacgttcct tgaatgctag agtcatttct
     5461 tgacaaggtt ttaattcttg atttttgctc gcatattgtg cgtcttttaa caggacatta
     5521 tctcttatag catgaagcat ctgtaatgtc tcaatatcat ttttttgaaa atatcgaaca
     5581 gtatctatgg ctataatggg tttggtatat ggaatctgcg gagaggtcaa attaacacca
     5641 ataacataat caaagggtaa attgagctga tcaagaacat cttgataggg aagaatcaat
     5701 tgcacccctt caagatgttc tgtcaataaa tttagagaca tcttctcaga catttgagcc
     5761 gttgacaact tcaagagatt cttataacca actgtattta tcgcaataca gtaaacttct
     5821 attgatctat tttcaaccac tatatcaaag ctaaatccaa ctattggtct taggtttgcc
     5881 ttttgcgcct cttgaataaa atgatatgct gcataaagat tactgtggtc caagatacca
     5941 atagtttgat atcctaatga tttcgattta gagacatatg tctttaaatc aaccaagcta
     6001 tccataaaag aataaacggt tcttgtatca agttgtgcaa acatagtagc ccctttttac
     6061 gattttttac tattatacta tgaaacaata aacgattaaa gtctcaatga taattccttg
     6121 acatttcaaa tatttttttg tactattttt atagtacaaa aagtacaaga ttatgaaagg
     6181 agttatctat ggcttgggaa ttcaatgaaa aatcacctat ttattcacaa attgctgaac
     6241 atataaagat gcaaattgtt agtcaagaaa taaaaagtgg tgaccaattg cctaccgtga
     6301 gagagttagc tcaagaagct ggtgttaacc caaataccat gcaacgtgcc ttcactgaat
     6361 tggaacgtga aggaatggta ttctcacaac gtacttccgg acgctttgtt acagaagaca
     6421 acttactaat tggtaaaatt cgtcaacaag ttgcaaaagc agagttagca acttttgtca
     6481 acaacatgaa aaaaatagga tataaattag atgaaattac ggttgctctc gatcatttta
     6541 taaaggagat taattaaatg acacagttat tacaacttca tcatgttaca aagaaatatc
     6601 acaaacatac tgctgttaat gatgtgactg taagtattcc aacaggaaag attataggtt
     6661 tattaggtcc aaatggtagc ggaaaaacaa caatcatcaa aatgataaat ggattacttc
     6721 agccagataa gggagatatc gttattgatg gttacagacc gtctgttgac accaaaaaaa
     6781 ttatttcata tctccctgat acttcatatt tacaagaaaa tatgaaaatc aaagacgtcg
     6841 ttacattatt tgaagatttc tataacgatt ttgattcaaa ggtagcatat caactatttg
     6901 aggatcttaa cctaaatcct agggaacgtc taaaaaacct atcaaaaggg aacaaggaaa
     6961 aagttcaatt aatacttgtt atgagtcgta aagctaggct ctacatcctt gatgaaccta
     7021 ttggtggtgt agaccctgca gcgcgtgact acattttaaa aacaatcatt agcaactact
     7081 ctaatgatgc ttctgtttta atttcgactc acttaatttc tgacattgaa cctatccttg
     7141 atgaagttat cttccttaaa gaaggagaaa ttgacctaca aggcaatgct gatgacttaa
     7201 gagaagagca caactgttcg attgatgctc tctttcgtga aagattcaag aaataaagga
     7261 gattgatatg tttggaaaat tattaaaata tgaattaaaa tcagttggta agtggtatct
     7321 aacactcaac gctgcggttt tattagtatc tattatttta ggactggtat taaaggcact
     7381 tggcggcaat ttttctactg atactaatag tactagcgcc caaatattca ctataatact
     7441 cgttttattg ttagcgatgg ttatttcagg atctctgtta tccacccttg ctattattat
     7501 caaacgtttt tatagcaata tcttcggccg tcaaggatat ctaaccttaa cgctcccagt
     7561 aactacgaat cagattattt gttcaaagct actagcttct cttctttgga gtatttttaa
     7621 tatttttatt gtcattattg gaatcatatt ggtcatatta cctttagtag gtattggtca
     7681 gttcgttgtc gcttttccag aaatctataa aattatttca tcttcgaacg ctccactttt
     7741 tatcgcctat ttctttctat cgtacgttgc agggacatta ctgatttatc tctctatcgc
     7801 tgtagggcaa ttatttacaa acaaacgtgt attgatggga attgtttctt attttggaat
     7861 atcactctta attactttct taacattaat aatagatagt atttttcata ttgatttatt
     7921 caactctcat gcaaatgcaa cattctctca acctgtattg ctctacaata tacttgttag
     7981 cattgttgaa attgctatat tctatatgtt gacacactct attattaagt acaaacttaa
     8041 tatacaataa aaagattgga gatatatctc caaccttttt ttatttttaa ttcaaaaaat
     8101 ttttcaaaat atcaccgcca taaatccaca aatagctata accgatgata gaaataggtt
     8161 tcgttatcat aatgattgtc acaaagcgct taatagacat attagataga ccggtaatca
     8221 tcaccatgat gtccgctggt gaaatgggag atgccataca aaagataaaa aacttttcat
     8281 atccggatgt ttctagctta gactcatatt tatcaaatgt cttctgatcc ataaatagca
     8341 aaacgaattt tcgtccataa aatttgacta accaaaaaag gatgacactt ccgatgataa
     8401 tcccaatata gttataaata aagcctaagg taggaccaaa aattaagaaa cctgctactg
     8461 ttgtaacacc tccaggaatt actggaaaaa caatctgtac aatttgagcc acaataaaaa
     8521 tcattggacc ccaaaattca tacttgtgta ctaggtcttt taattcattg ctatcattca
     8581 aaataccaag tttgtacaaa taaacaacta agacaagagt cgctattaac gctaagacac
     8641 ccaaagcctt aataaccttc tgccaaaagc ggtaacgttt tgataatttc atattcatgc
     8701 tttaatcata acacaactgt taaatttttg ctattttaaa tagtatttgt tatactatat
     8761 tggtaagttt ttatgcttta ttctactttt ggggtcgtta cggattcgac aggcattatg
     8821 aggtatattt cgcgactcat cggcagatgt aaaatgccag ttaaatataa ctgcaaaaaa
     8881 tacaaattct tacgcattag ctgcctaaaa aacagcctgc gtgatcttca caagattgtt
     8941 tgcgttttgc tagaaggtct tatttatcag caaactacgt ttggctactg tctagttagt
     9001 taaaaagaga tttatagact cgctatgtga gggcttgagt tatgtgtcat cacctagtta
     9061 aatcaataca taacctatag ttgtagacaa atatattagc agatgtttgg acgtgggttc
     9121 gactcccacc ggctccatat cattttacca taacttacta tacatactta aaaccttgtt
     9181 attacaaggt tttaagtatg tttctttgtt catacattgc ctctatctta ggaaaaaaat
     9241 actgggaaca gactctaaca ttgaattagg ccacctcatt agctcactct ttgtcaatat
     9301 ccgagaataa gttctttatc attctaattt atagaattta ttaaaaaagg taaggttttt
     9361 agaggaaaaa atgttataat ctatttagtc tacgattgga ggctagttat ggctagtatt
     9421 ttaaaaaaac atttagagaa acgtttagag cttaaaaatg atcctatgag gacagatatg
     9481 agtactggtg ccagttaccc taaatatgga ttctcggtag gaaagtacac atatggatat
     9541 caacaattct tttacgaagg cgttaactta aaagaaattg gagcattttg ctcaatagcc
     9601 cagaacgtta ctattactgg tctgaatcat ccaaccgatc acattaccac taaccctttt
     9661 atttactata agtcaagggg atttataaat gaggataggg cggacttaat agatgaaaag
     9721 aaaaatggaa aagttattat tggcaacgat gtctggatag gaactaatgt cactatactt
     9781 ccatctgtta caataggtaa tggagcaatt attggagcag gttcagttat taccaaagac
     9841 attcctgact atgctgttgt cgctggaaca cctgctaaaa ttatcaaata tcgcttttca
     9901 gaggaagaaa taacattatt aaatgcatct cagtggtgga attggtcaga tgaagctatt
     9961 aaggaacata ttagcgaatt ttcagataag aaggaattct tcaatactct aaaaagtatt
    10021 tccgaaaaca aaaatcataa actctagaat aacttcat
//
LOCUS       DATRMA010000069        10046 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.069, whole genome shotgun sequence.
ACCESSION   DATRMA010000069 DATRMA010000000
VERSION     DATRMA010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10046
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.069"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            complement(128..907)
                     /locus_tag="VBC28_001620"
     CDS             complement(128..907)
                     /locus_tag="VBC28_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEO6815588.1"
                     /translation="MSKVKVLSLITVSGLFLMAGNLSASADVVTSGGDTIMLSGVDAG
                     VSDSITPPPSSINPVTDTTEPSAPTPSTDPITDTTEPSAPTPSTVPITDTTEPSAPTP
                     STDQTTDTTDSSTSAPSTDQTTDATDSSTSAPSTEAEKPAPSKEPDAPKPITKPIVDV
                     PISTVTGDTVIGTENGKVIVQSASGTILKDAKEVGGEVQKDGTVAIKKSDGKIEVLPN
                     TGEGKTIFTIVGLLLIAGAVWIGFKDNIKKYLTFFKKKSEK"
     gene            complement(924..1472)
                     /locus_tag="VBC28_001621"
     CDS             complement(924..1472)
                     /locus_tag="VBC28_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6815589.1"
                     /translation="MKRRKIVLFLMVLVMAFIVYKVIQKDNPVIKTSGNHTKVERVEP
                     KHKVKSYDSTKEAKSTLEASDRQVSDKEKEKVKKSITVPIDYLVKIPTQSDIKGDYQN
                     KLSITNPTVAQTVKTMLMAGYHFDLESLQVYESDASNVYQFTINLVTDKRDKLSLAGN
                     YVLGTDQFEIASVHGTPVNVVF"
     gene            complement(1472..2845)
                     /locus_tag="VBC28_001622"
     CDS             complement(1472..2845)
                     /locus_tag="VBC28_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006740735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEO6815590.1"
                     /translation="MGILIVFPFLLFLLLIASSSSQQDCQITPDVSSQVETSSENASN
                     SDWTKKGSVANKTAEKVFNAWVSKGLSGASASGIVGWTNSEGGFAMIGRAEGHYGNDL
                     KTNSIAFNVRPTGLSYYTTEAGGGIYQITPFTKYAPLGDPKWEDADAMNAFVMKSIKS
                     GDWNKAMDLSGGNHSFEEMAQMTDPKQATLVWNAYERGNTAYINKAQKSADGQKAYDM
                     FNGSKYKFDAERFSKSFSGGSKTSSSGKSDIISDSTVLSLCDSASNAVQGLFGKDKSG
                     KVKYTSYNAWKPDQLPSDLKKYAIDPKSVGLSYRTSSGWNAIASTGGQCTDLSASLMY
                     GLWLKGGMHPTQRMGNGNMVVSNWVSAFGGRSENTPSSGAVFSSSGTSSAGHTGVVSH
                     VFENGDMLIVEQNYSTYSGDNGGFGPYSWNYRYVSTLELKKEHYSFYNLSKAGYKLSD
                     KVKTVGS"
     gene            3009..3152
                     /locus_tag="VBC28_001623"
     CDS             3009..3152
                     /locus_tag="VBC28_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004239191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HEO6815591.1"
                     /translation="MGNSSKSNRKEELDLSTFEVLTLMFIAGNFVIALVKLVLELVKS
                     TKK"
     gene            complement(3343..5361)
                     /locus_tag="VBC28_001624"
     CDS             complement(3343..5361)
                     /locus_tag="VBC28_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6815592.1"
                     /translation="MDKIDDGLIASTFAKVKDVDIFALKAYMEITHGAETGAQSILLD
                     VFVNFPFFLLNLIVGLFSVILRFFENFSLYDTYKQTVYHSSQKLWENLSGNGSYTSSL
                     LYLLVAISAFSIFISYLFSKGDFSKRLIHLFVVIILGMGYFGTIQSTSGGIYILDTVH
                     QLAGSFSDAVTNLSLDNPSGGKTKITQKSSVADNYVMKTSYTAYLFVNTGQLNGKFHN
                     NQTGKEEKFDNEQVLGKYDKSGKFITPKQKDILNYTDNLGDKATEGEEKNRWLSAVND
                     YLWIKSGYVILKIFEAVILAVPLILIQLIAFMADVLVIILMFIFPLALLVSFLPRMQD
                     IIFNVLKVMFGAVSFPALAGFLTLIVFYTQTLIATFVKKKFTDGSLLSGSNFKGQAIL
                     FMLLITVFVQGCVFWGIWKYKETFLRLIIGSRASQVINQSADRINEKANDLGITPKSI
                     YEKAHDMSNLAMMGAGYGVGTIMNAQDNWNAFKERRQGKSENHPSQGEDPNHYDEVNA
                     DESRVSKDDSLHQAPDYQLELPNENGQSFHPKTTDRTYDVPFEPDKMRTDDVLNDVET
                     DNHMFNEANSNMSMREHESKTVCSEDLPNSRTRITVGVSPLNQDKIEKMKGDLNQYNS
                     DLNVTKNHGRTPFEKGFNASKTKEVRKRHNLERQSKVLEELEKLRGAK"
     gene            complement(5377..7878)
                     /locus_tag="VBC28_001625"
     CDS             complement(5377..7878)
                     /locus_tag="VBC28_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEO6815593.1"
                     /translation="MVKKLEYPIDRIHQNLALRKDKVVVAYYRIPNTPITITDSDKKD
                     SHKAKVAQVIKKLAKYKNFDISLIPKDYLLGEKMSDFEADLADDVKELGLETLDFTVD
                     TLTHEMEIPYQFDWLIGVDLGKGQYNANIKEFIYNQFESIASNFASLAGYEVEVDEDW
                     YKEHSEEELLVYSLLSTLKAKRLTDVDLFYYQRMQFLRYVPHTKSEVIANRNMLNVTD
                     TLIKSLEGGFLKLESAYGSSFVSVLPVGRFSTIFNGFHLGELVQRMSFPVELRFQAEF
                     IDKTKLGGTMGRSNTRYDQIMKEAYNTNTVQQDDILMGAYSLKDLMKKVGNKEEIIEY
                     GCYLVVAGSSLNQLKQRRYAILSYFDDMKVNVYEASHDTPYLFQALLYGQDLQKTTRK
                     WNHLVTARGFSELMLFTNTQSGNRIGWYIGRVDNRLTAWDSIDEAIMGSKNLVLFNAT
                     VANKEDVAGKVTKNPHVIITGATGQGKSYLAQMIFLHTAQQNVRVLYVDPKRELRQHY
                     LKVVSDAEYARKFPLRKKQIGEMNFVTLDSSVKENHGVLDPIVILDKEGASSTAKNML
                     LYLLKNATEIKLDQTTALTEAISQVIAKREAGEVVGFNQVIEALIDSESDEVQSVGRY
                     FKAIIQNSILELAFSDGDVAGLSYEERVTVLEVADLSLPKDGSDHISDHESNSIALMF
                     ALGAFCKHFGERSDDETVEIFDEAWVLMQSSEGKAVIKSMRRVGRSKYNVLMLVSQSV
                     HDAENDDDTTGFGTIFSFYEKSEREDILRHVGLEVTPKNLEWIDNMISGQCLYYDVYG
                     NLNMISIHNIHPDIDPLLKPMKKTVSSHLENKYAS"
     gene            complement(7899..8309)
                     /locus_tag="VBC28_001626"
     CDS             complement(7899..8309)
                     /locus_tag="VBC28_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEO6815594.1"
                     /translation="MEEEKYFDYSRGLNAPYWIQEVKTAKGKVIWYFSTPMQLSFFIV
                     FFIILMLMLTVCSPLMQVLNALTHSVSMLLYWFVPSKLAKFYVEYEPQGKKMHIFLWD
                     YLVYLKDFGLNKKGIYQGERVDIVEEIVFEKTKI"
     gene            complement(8313..8579)
                     /locus_tag="VBC28_001627"
     CDS             complement(8313..8579)
                     /locus_tag="VBC28_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001055037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6815595.1"
                     /translation="MNLVKVNSVILAGGPNVSGLKQFFQGDGIYLISVIAVFIIIKNW
                     KEANWLKVFSTLAIYAIIVSMLKGQQILSFLGGLLRWFGVETGL"
     gene            complement(8599..9552)
                     /locus_tag="VBC28_001628"
     CDS             complement(8599..9552)
                     /locus_tag="VBC28_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEO6815596.1"
                     /translation="MIDIQKIKKFLLRYTKKEEGGKQPKTKEVKQRTANFIVYGILGL
                     LFIVGFFGSLRAIGLSNQVQHLKETVIAVEKKSKHKKTDDSLDISRIQYYMNNFVYYY
                     INYSQDTADQRKTELENYYSFSTASMTDDVRKSRTLRTQRLISVEKEKDYYIALMRIG
                     YEVDKKSYQMNLAVPFQMQRGLLAIVSQPYTVAEDLYLGKSKAFEKKTLDQVKELSKE
                     QVSSIQKFLPVFFNKYVLSNKTDLKLLMKTPELMGKGFKVSELDLNNAIYYQEKKHQV
                     VQLSVTFSDLVTGGTRSENFTLYLSKADNGWYVEEMYHYFK"
     gene            complement(9605..9859)
                     /locus_tag="VBC28_001629"
     CDS             complement(9605..9859)
                     /locus_tag="VBC28_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002279547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HEO6815597.1"
                     /translation="MFNFTEEAWKDYVSWQQEDKKILKRINRLIEDIKRDPFEGIGKP
                     EPLKYHYSGAWSRRITEEHRLIYMIEDGEIYFLSFRDHYK"
     gene            complement(9852..>10046)
                     /locus_tag="VBC28_001630"
     CDS             complement(9852..>10046)
                     /locus_tag="VBC28_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HEO6815598.1"
                     /translation="EPLIVVNKNPDENIVVLSQDSWESLQETIRLMENDYLSHKVING
                     ISQVKEKQVTKHGLIEVEDV"
BASE COUNT         3228 a         2008 c         1365 g         3445 t
ORIGIN      
        1 atgtgtgtcc tcctttttta cagttattat atcatatata aagaaaacac aacatatttt
       61 tcgaaaaact taaagtaaag aatttaacat ataaaattaa agccatgaaa tgaatcatgg
      121 cattatctta tttttcactt ttctttttga aaaaggttaa atactttttt atattatctt
      181 taaatccaat ccaaactgct ccagcaatta acaagagacc tactattgta aagatagtct
      241 taccctcacc agtgtttggc aatacttcta tttttccatc tgatttttta attgctacag
      301 taccgtcttt ttgtacctca ccaccaacct ctttggcatc ctttagtatt gttccagatg
      361 cactttgaac aatcactttt ccattttcag tgccaattac tgtatctcct gtaactgttg
      421 aaattggaac atcaacaata ggttttgtaa taggcttagg cgcatctggc tctttactag
      481 gtgctggttt ttctgcttct gtacttggtg ctgacgtact tgaatctgtc gcatctgttg
      541 tttgatctgt acttggtgct gacgtacttg aatctgtcgt atctgttgtt tgatctgtac
      601 ttggtgttgg tgcacttggt tctgtggtat ctgttattgg aactgtactt ggtgttggcg
      661 cacttggttc tgtggtatct gttattggat ctgtacttgg tgttggcgca cttggttctg
      721 tggtatctgt tactggattt atacttgatg gtggtggcgt tatagaatca gatacacctg
      781 catctacccc tgatagcatt atagtatcac ctccactagt tacaacatct gcacttgctg
      841 ataaattacc agccattaaa aatagtcctg aaacagtaat taaacttaaa acttttactt
      901 ttgacataaa ttttccttct ttcttaaaat actacattta caggtgtacc atgtacactc
      961 gcaatctcaa attggtctgt acctaataca taattaccag ctaaacttaa tttgtctctt
     1021 ttatctgtta ctaaattaat agtaaactga taaacattac tcgcatctga ctcatatact
     1081 tgtaaacttt ccaaatcaaa atgataacca gccatcaaca tggtttttac agtttgggca
     1141 actgtaggat ttgtaatgga taatttattt tgataatcac ccttaatatc tgattgtgtt
     1201 gggattttta caaggtaatc aataggtaca gttatagatt ttttaacctt ctccttttcc
     1261 ttatcagata cttgacgatc actagcttcc aaagtagatt ttgcttcttt agttgaatca
     1321 taagacttaa ccttatgttt cggttcaact cgttcaactt ttgtatgatt accacttgtt
     1381 ttaatcactg gattatcttt ttgaataact ttataaacta taaaagccat cactaacacc
     1441 attaaaaata aaactatttt tctacgtttc attaactacc taccgttttc actttatcag
     1501 acaatttata accagcttta gagagattat aaaaactata atgctctttt tttaattcta
     1561 aagtagaaac ataacgataa ttccatgaat atggtccaaa tcctccatta tctccagaat
     1621 aagttgaata attttgctct actattaaca tatcgccatt ttcaaataca tgactaacca
     1681 ctccagtatg cccagcacta gaagtacctg aacttgaaaa aacagctcca gaagatggag
     1741 tattttcaga gcgtccaccg aatgcagata cccaattaga aacaaccata ttaccattgc
     1801 ccattctttg cgttggatgc attccgcctt taagccataa accatacatc aaacttgctg
     1861 aaagatctgt acattgacca ccagtagaag ctatcgcatt ccatccacta gacgttcgat
     1921 aagataaacc aactgactta ggatctattg catacttttt caaatcactt ggtaattgat
     1981 ctggtttcca tgcattataa gaagtgtatt taactttccc tgacttatct ttaccaaata
     2041 agccctgtac cgcattacta gctgaatcac aaagagataa aactgtagaa tcagaaatta
     2101 tatcgctctt accagacgag gacgtttttg aaccaccact aaaactttta gaaaatcttt
     2161 ctgcatcaaa tttatatttt gaaccattaa acatgtcata ggctttttgc ccatccgcag
     2221 acttttgagc tttattaata taggcagtat ttcctcgttc gtaagcattc caaactaaag
     2281 tagcttgttt cggatctgtc atctgtgcca tctcttcaaa agaatgattt ccacctgata
     2341 aatccattgc tttattccag tctcctgatt taatggattt cataacaaag gcattcatgg
     2401 catctgcatc ttcccatttg ggatcaccta gcggtgcata ttttgtaaaa ggcgtaattt
     2461 gataaatacc acctccagct tcagtcgtat agtaagataa cccagtaggt ctcacattaa
     2521 aggcaattga gtttgttttt aaatcattac cataatgtcc ttctgctcga cctatcatcg
     2581 caaagccacc ttctgaattt gtccaaccaa ctattcctga tgcacttgct ccagataaac
     2641 ctttacttac ccacgcatta aataccttct ccgcagtctt attagcaaca gagccttttt
     2701 tagtccaatc actattggaa gcattctcac ttgaggtttc aacttgtgaa gatacatctg
     2761 gtgtaatttg acaatcttgt tgagaacttg acgaagcaat taacaataaa aacaataaaa
     2821 aaggaaatac gatcagtatt cccaaacagc ctaatttttt cttatgtttt actttagttc
     2881 cctcctttca aacaaaataa aaaagcacca acaaaaattg atactttaaa ccactatgaa
     2941 atatgatata ataatgaagc agaaaagtta agacggtggc tcttatttct gaaaggtagg
     3001 tggtgcccat gggcaattca tcaaaatcca acagaaagga ggagctagat ttgtctacct
     3061 ttgaggtctt aacactcatg tttatagcag gtaacttcgt tatcgctctc gtgaagttag
     3121 tccttgaact ggtaaaatcg acaaaaaaat aaccgtctca actttggcga gttaacggtt
     3181 attttttaac ggttattttc tgccaccgtc ttaaacggtt ctgctaagca gttgttagaa
     3241 gcaactgctt ttttctatac tagtattata tattatatta gaaatagtgt caattgggat
     3301 acttagttta ctaaacctct caatgtcata aaactcctta tgctacttag ctcctctcaa
     3361 cttttctagt tcttccaaaa cttttgactg tctttcaaga ttatgtcgct ttctaacttc
     3421 tttcgtttta ctggcattaa atcccttttc aaaaggagtt ctaccatgat ttttagtcac
     3481 atttagatca ctattatact ggtttaagtc acctttcatc ttttctattt tatcctgatt
     3541 caaaggagat acaccaacag taattcttgt tcttgaatta ggcagatctt ctgaacaaac
     3601 agtcttactt tcatgttctc tcatagacat attactatta gcttcattaa acatatgatt
     3661 atccgtctct acatcattca agacatcatc tgttcgcatc ttatccggtt caaaaggaac
     3721 atcataagtt ctatcagttg tctttggatg aaatgattgg ccgttttcat ttggcaactc
     3781 taattgataa tctggtgcct gatgtaatga atcatcttta gataccctag attcatccgc
     3841 atttacttca tcataatggt taggatcttc accttgagaa ggatgatttt ctgattttcc
     3901 ttgtcgtctt tctttaaaag cattccaatt atcctgtgca ttcataatag tgccaacacc
     3961 ataaccagcc cccatcatag caaggtttga catatcatga gccttctcat agattgattt
     4021 tggagtaata cccaaatcat ttgctttttc atttattcta tctgcagatt gattaataac
     4081 ttgagaagct ctagacccta taatcagtct taagaaagtt tctttatatt tccaaattcc
     4141 ccaaaacaca caaccttgga cgaaaactgt tattagcaac ataaacagta ttgcctgacc
     4201 tttaaaatta ctaccactaa gcagggaacc atctgtaaat tttttcttaa caaaggtggc
     4261 tattaaagtt tgagtataga agactatcaa ggttaaaaaa ccagctaagg caggaaagga
     4321 aacagcccca aacatgactt tcaaaacatt gaaaatgata tcttgcattc taggcaaaaa
     4381 tgaaactaat aaagctaatg gaaaaataaa cattaatata atcactaaaa catctgccat
     4441 aaaagcaatt aactgaatga gtattaatgg aactgcgagt ataaccgctt caaaaatctt
     4501 taagataaca tagcctgatt ttatccaaag ataatcattt actgcagaaa gccatctatt
     4561 tttttcctcc ccttcggttg ccttatctcc caaattatca gtataattca atatatcctt
     4621 ttgttttgga gtgataaatt tacctgattt atcgtactta cctaaaactt gttcgttatc
     4681 aaacttttct tctttacctg tttgattatt gtgaaactta ccatttaact gaccagtatt
     4741 gacaaacaaa taagccgtat aagaagtttt catcacataa ttatcagcta ctgatgattt
     4801 ttgtgtgatc tttgttttac cacctgatgg attatccagt gataaattag ttaccgcatc
     4861 tgaaaacgaa ccagctagtt gatgaacggt atctaaaata taaataccac ctgatgttga
     4921 ttgaatcgta ccaaagtaac ccatacctaa aatgataacc acgaacaagt ggatcaaacg
     4981 tttagaaaaa tctccttttg aaaaaagata tgaaataaat attgaaaaag ctgaaatcgc
     5041 aaccaataaa taaagcaagg agcttgtata agacccatta cctgatagat tttcccataa
     5101 tttttgagac gaatgataaa ctgtttgttt ataggtatca tacaaactga agttttcaaa
     5161 aaagcgtaga ataacagaaa ataaaccaac aatcaagtta agtaggaaaa agggaaaatt
     5221 aacaaaaaca tctaacaaaa tgctttgagc tccagtctca gctccgtgag ttatctccat
     5281 ataagccttt aacgcaaata tatccacatc ttttaccttc gcaaatgtcg aagcaatcaa
     5341 accatcatct attttgtcca tctgattacc tcccttctac gaagcatatt tattttcaag
     5401 gtgacttgat actgttttct tcataggttt taacaatgga tcaatatctg gatgaatgtt
     5461 atgtatcgaa atcatattca agttaccgta aacatcataa taaagacatt gacctgaaat
     5521 catattatca atccattcta gattcttagg tgttacttcc aaaccaacat gccttaaaat
     5581 atcttcacgt tccgattttt cataaaatga aaaaattgtc ccaaaaccag tcgtatcgtc
     5641 atcattttcg gcatcatgaa cagattgaga cactagcatc aacacattat acttagagcg
     5701 accaacacgg cgcatagatt taataacagc ttttccttct gatgactgca tgagaaccca
     5761 agcctcatca aaaatttcaa ctgtttcatc atcactacgc tcaccaaaat gcttacaaaa
     5821 cgcacctaaa gcaaacatta aagcaatcga attactttca tgatcagata tatgatctga
     5881 accgtcctta ggcagtgata aatccgcaac ttctaaaact gtcacacgtt cttcgtaaga
     5941 aagaccagca acatcaccat ctgaaaatgc caactctaaa atagaattct gaataatagc
     6001 tttaaaataa cgaccaactg actgaacttc atcactttca gaatcaataa gtgcctctat
     6061 cacctgatta aaaccaacga cttcaccagc ttcacgtttt gcaatgactt gactaattgc
     6121 ttctgttaaa gctgttgttt gatctaattt aatctctgta gcgtttttta acaaataaag
     6181 caacatattt ttagccgttg aggaagcacc ttccttatca agaataacaa taggatctaa
     6241 tacaccatga ttttctttga cagaactatc aagagtgaca aaattcattt ctccaatttg
     6301 cttcttacgt agaggaaact tacgtgcata ctctgcatca gaaacaactt ttaaatagtg
     6361 ttgccgtagt tctcgtttag ggtcaacata aaggacacga acattttgtt gagcagtatg
     6421 taaaaagatc atctgtgcta agtatgattt cccttgacct gtcgccccag ttataatcac
     6481 gtgaggattt ttagtaacct taccagcaac atcttcttta ttcgcgactg tcgcattaaa
     6541 taagacaaga tttttagatc ccataatagc ttcgtcaata gaatcccaag cagttaaacg
     6601 attatctact cgtccaatat accaaccaat cctgttacct gattgggtat ttgtaaaaag
     6661 catcaactcc gaaaaacctc gagcagttac taaatgattc cacttacgag ttgttttttg
     6721 taaatcttga ccataaagta acgcttgaaa taaatatggt gtatcatgac tggcttcata
     6781 aacattgact ttcatatcat caaagtaact taaaatggca tatctacgtt gttttagttg
     6841 atttaaacta gaaccagcaa ctaccaaata acaaccatac tctatgattt cttccttatt
     6901 tccaactttt ttcataagat ccttaagcga atatgctccc atcaagatat catcttgttg
     6961 aaccgtattt gtgttataag cttctttcat gatttgatca tagcgtgtat ttgagcgacc
     7021 catagtgcca cccaattttg tcttatctat aaattccgct tgaaatctta attcaaccgg
     7081 aaaactcatt ctttgaacta gttcaccaag atgaaagcca ttaaatatgg tagagaaccg
     7141 acctactgga agaacggata caaatgaact cccataagca ctctccaatt ttaaaaaacc
     7201 accttctagt gatttaatca acgtatctgt cacatttaac atattccgat tagctataac
     7261 ctcactttta gtatggggaa catatcttaa aaattgcatc cgttgataat aaaataaatc
     7321 aacatccgtt aatcttttgg ctttcagggt agacaataaa ctatagacaa gtaattcttc
     7381 ttctgaatgt tctttatacc aatcttcatc tacttctacc tcataaccag ctaaagaagc
     7441 gaagtttgat gcgatagatt caaactgatt ataaataaat tcttttatat tagcattgta
     7501 ttgcccttta ccaagatcaa ctccaattaa ccaatcaaac tgatacggta tctccatttc
     7561 atgtgttaac gtatcaactg taaaatcgag tgtttctaac cctaattctt taacatcatc
     7621 tgctagatcc gcttcaaaat ccgacatttt ttcaccaaga agataatctt ttggtattaa
     7681 tgaaatatca aaatttttat atttagctaa ttttttgata acctgtgcta ccttcgcttt
     7741 atgactatct tttttatcac tatctgtaat agtgattggt gtatttggaa tgcgatagta
     7801 tgcgacaaca actttatctt ttcgtaaagc taaattttga tgtattctat caatcggata
     7861 ttccaatttt tttaccatct gatctccttt cttagtcttt atattttcgt cttctcaaaa
     7921 actatttcct ctacaatgtc aacacgttct ccttgataaa tccctttttt atttaaccca
     7981 aaatctttga gataaaccag ataatcccac aaaaagatat gcatcttttt tccttgcggt
     8041 tcatattcta cataaaattt cgctaacttg cttggaacaa accaatacaa gagcattgaa
     8101 acggaatgcg ttaaagcatt taacacctgc attaatggag aacataccgt cagcatcaac
     8161 attaagatga taaagaaaac aataaaaaat gataactgca taggtgtaga aaaataccaa
     8221 atcactttgc cttttgccgt tttaacttcc tgaatccagt aaggagcatt caagcccctg
     8281 ctgtaatcaa aatatttttc ttcttccata acttataacc ccgtctcaac accaaaccag
     8341 cgaagcaatc cacctaagaa agatagaatc tgttgacctt taagcatcga tacaataatg
     8401 gcataaatag ctaaagtaga gaaaactttt aaccaattag cttctttcca attcttaata
     8461 attataaaga cagcaattac actaatgagg taaatcccat caccttgaaa aaattgcttt
     8521 agtcctgaga cgttgggacc acctgctaaa ataacactat tcacttttac taaattcatt
     8581 ttttcctctc ttacacctct atttaaaata atgatacatc tcttcaacat accaaccatt
     8641 gtctgcttta gataaatata atgtaaagtt ttctgatcgt gtgccacctg ttacaagatc
     8701 agaaaaagtt actgataatt gcacgacttg atgctttttt tcttgataat aaatcgcatt
     8761 attaagatct agctcactaa ctttaaatcc tttccccatc agttctggag ttttcattaa
     8821 cagtttcaag tctgtcttgt tacttaagac atatttatta aagaaaactg gtaaaaactt
     8881 ttgaatagaa cttacttgtt ctttcgatag ttccttgact tgatctaatg ttttcttctc
     8941 aaatgcttta gatttaccta aatacaaatc ttctgccaca gtataaggtt gactaacgat
     9001 tgcaagcaat cctctttgca tctgaaaagg aacagccaag ttcatctgat atgatttttt
     9061 atcaacttca taaccaatac gcatcaaagc tatatagtaa tctttttctt tttcaacact
     9121 tattaaacgt tgtgtccgca aagtcctaga ctttctaaca tcatctgtca tactggcagt
     9181 tgagaaagaa taataatttt ctaattctgt ttttctttga tctgcagtat cttgtgaata
     9241 gttgatatag tagtagacaa aattattcat gtaatactga attctagata tatctaatga
     9301 atcgtcagtt ttcttatgtt ttgacttttt ttctacagca ataactgttt cttttaaatg
     9361 ttgtacctga ttagataagc caattgccct caatgatcca aaaaatccta caataaataa
     9421 caaacctaag ataccataga caataaaatt ggctgtcctt tgctttactt cttttgtctt
     9481 aggctgttta cctccctctt ctttctttgt atatctcaat agaaactttt ttattttttg
     9541 tatatcaatc atttaacccc tttctataaa ataaaaaagc agtcactttt tttagcgact
     9601 acttctactt ataatgatct ctaaaagata aaaaatagat ttctccatct tctatcatat
     9661 aaatcaacct atgttcttct gtaattcttc gagaccaagc ccctgaataa tggtatttta
     9721 aaggttctgg ttttccaatg ccttcaaatg ggtctctttt aatatcttct atcaaacgat
     9781 taattcgttt caagattttt ttatcttcct gttgccaaga aacataatct ttccatgctt
     9841 cttcagtaaa attaaacatc ttctacctca attaaaccat gtttagtgac ttgcttctct
     9901 ttaacttgtg atataccatt aataacctta tgtgatagat aatcattttc catcaaacga
     9961 atcgtttctt gtaaactttc ccagctatct tgagataaaa ccactatatt ttcatcagga
    10021 tttttattaa ctacaatcaa aggctc
//
LOCUS       DATRMA010000070         9987 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.070, whole genome shotgun sequence.
ACCESSION   DATRMA010000070 DATRMA010000000
VERSION     DATRMA010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9987
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.070"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            378..1340
                     /locus_tag="VBC28_001631"
     CDS             378..1340
                     /locus_tag="VBC28_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEO6815599.1"
                     /translation="MKKITKMVISSVLATVLMTACSQSSQNGQSDVKHIGILQYVEHP
                     SLTATRKGFIKELAKEGYKDGKNIKIEYKNAQGDQSNIQSISEKLIKDNKLVLGIATP
                     AAQSLTTVSTETPILFTAVTDPVSAELVKSMKKPEGLATGTSDMSPIKKQVSLLRKVM
                     PKVKRVGIMYTTSERNSEVQVKQAKKIFQEAGIKTSVKGISSTNDVQDTAKSLMSKTE
                     VIFVPTDNIIASSVTLLGNLSKELKVPVVGGSADMVPSGLLFSYGADYEALGRQTARQ
                     AVKILKGKDVAKVPSEYPQNLKVVVNEDMAKELGIDVSSIKNNK"
     gene            1356..2252
                     /locus_tag="VBC28_001632"
     CDS             1356..2252
                     /locus_tag="VBC28_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6815600.1"
                     /translation="MLDIILSGISQGLLWSIMAIGVFITFRILDIADLSAEGAFPMGA
                     AVCALCIVNDINPIVATIAGMLGGMLAGLVSGFLHTKMKIPALLTGIITLTGLYSINL
                     LVLGRSNVSFALKNTLVTMVTRLGLNKLSAVLLIGIVCVGLVILILYLFLNTQLGLAL
                     RATGDNEAMGQANSIKVDRMKMLGYMIGNGLIALSGALLAQNNGYADLNMGVGTIVIG
                     LASIILAEVMIKYLPLGKRLWSIVLGSVLYRMIIVFILTTDIDAQMIKLVSAILLALI
                     LYVPELRAKLGVKTSKSLTKEG"
     gene            2257..3060
                     /locus_tag="VBC28_001633"
     CDS             2257..3060
                     /locus_tag="VBC28_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000054433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO6815601.1"
                     /translation="MSLLELVNLHKTFEKGTVNENHVLRGLDLTIEDGDFISVIGGNG
                     AGKSTLLNCIAGLIPIDQGAITLDNQSITKDSVEKRSKDISRVFQDPRMGTATNLTIE
                     ENMAIAHKRGNKRHIFRQSVTDDDRQLFKKSLSQLGLGLENRMKTDAAFLSGGQRQAL
                     TLAMATLVRPKLLLLDEHTAALDPKTSDMVMELTQKVIEEQRLTALMITHNMEHAIAY
                     GNRLVMLYHGKIVVDVKGEAKRNLTVAELMELFHKNSGQQLIDDALVLG"
     gene            3235..3519
                     /gene="groES"
                     /locus_tag="VBC28_001634"
     CDS             3235..3519
                     /gene="groES"
                     /locus_tag="VBC28_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEO6815602.1"
                     /translation="MLKPLGDRVIISFVETEEKSVGGFVLAGASHDATKTAKVLAVGD
                     GIRTLTGELVAPSVAEGDTVLVENGAGLEVKDGNEKVTVVRESDIVAVVK"
     gene            3615..5237
                     /gene="groL"
                     /locus_tag="VBC28_001635"
     CDS             3615..5237
                     /gene="groL"
                     /locus_tag="VBC28_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEO6815603.1"
                     /translation="MAKDIKFSADARSAMVRGVDILADTVKVTLGPKGRNVVLEKAFG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPIGIRRGIETAVSAAVEELKEIAQPVSGKEAIAQVAAVSSRSEKVGEYI
                     SEAMERVGNDGVITIEESRGMETELEVVEGMQFDRGYLSQYMVTDNEKMVSELENPYI
                     LITDKKISNIQEILPLLEEVLKTNRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVVTEDLGLDLKDATMQVLGQSAKVTVDKDSTVIVEGA
                     GDSSAIANRVAIIKSQMEATTSDFDREKLQERLAKLAGGVAVIKVGAATETELKEMKL
                     RIEDALNATRAAVEEGIVSGGGTALVNVIEKVAALKLNGDEETGRNIVLRALEEPVRQ
                     IAYNAGYEGSVIIERLKQSEIGTGFNAANGEWVDMVTTGIIDPVKVTRSALQNAASVA
                     SLILTTEAVVANKPEPEAPTAPAMDPSMMGGF"
     gene            complement(5218..5633)
                     /locus_tag="VBC28_001636"
                     /pseudo
     CDS             complement(5218..5633)
                     /locus_tag="VBC28_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181221.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5646..6383)
                     /locus_tag="VBC28_001637"
     CDS             complement(5646..6383)
                     /locus_tag="VBC28_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEO6815604.1"
                     /translation="MPKNELNNKLNKLKHVQVYNKIFNMIQDGTYSPGMQLPSEPELA
                     GQLNVSRATLRKSLALLQEDHLVKNIRGKGNFIRENSSNLSENGYENRQHPIKTCLTS
                     KITEVELEFRVEVPAEAITASLKQETPVVVIADRWYHTDDGPLAYTLSFIPIELISDA
                     EISLHDTKQLLNFIEEGIYQEGISSHSQSHLGYATSGNFSATKYTLSDHGQFILIQET
                     IFKQEKILMCNKHYVPIEHFELSITSH"
     gene            6541..7320
                     /gene="udp"
                     /locus_tag="VBC28_001638"
     CDS             6541..7320
                     /gene="udp"
                     /locus_tag="VBC28_001638"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HEO6815605.1"
                     /translation="MQNYSGEVGLQYHLQIRPGDVGRYVIMPGDPKRCAKIAEHFDNA
                     VLVADSREYVTYTGTLNGEKVSVTSTGIGGPSASIAMEELKLCGADTFIRVGTCGGID
                     LDVKGGDIVIATGAIRMEGTSKEYAPIEFPAVADLEVTNALVNAAKKLGYTSHAGVVQ
                     CKDAFYGQHEPERMPVSYELLNKWEAWKRLGTKASEMESAALFVAASHLGVRCGSDFL
                     VVGNQERNALGMDNPMAHDTEAAIQVAVEALRTLIENDKSQ"
     gene            7341..8543
                     /locus_tag="VBC28_001639"
     CDS             7341..8543
                     /locus_tag="VBC28_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HEO6815606.1"
                     /translation="MQFIYSIIGILLVLGIVYAISFNRKSVSLSLIGKALIVQFIIAL
                     ILVRIPLGQQVVSVVSTGVTKVINCGQAGLNFVFGSLADSGAKTGFIFAIQTLGNIVF
                     LSALVSLLYYVGILGFVVKWIGKGVGKIMKSSEVESFVAVANMFLGQTDSPILVSKYL
                     GRMTDSEIMVVLVSGMGSMSVSILGGYIALGIPMEYLLIASTMVPIGSILIAKILLPQ
                     TEPVQKIDDIKMDNKGNNANVIDAIAEGASTGAQMAFSIGASLIAFVGLVSLINMMLS
                     GLGIRLEQIFSYVFAPFGFLMGFDHKNILLEGNLLGSKLILNEFVSFQQLGDLIKSLD
                     YRTALVATISLCGFANLSSLGICVSGIAVLCPEKRGTLARLVFRAMIGGIAVSMLSAF
                     IVGIVTLF"
     gene            8573..9244
                     /gene="deoC"
                     /locus_tag="VBC28_001640"
     CDS             8573..9244
                     /gene="deoC"
                     /locus_tag="VBC28_001640"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEO6815607.1"
                     /translation="MEVKDILKTVDHTLLATTATWPEIQTILDDAMAYETASACIPAS
                     YVKKAAEYVSGKLAICTVIGFPNGYSTTAAKVFECQDAIKNGADEIDMVINLTDVKNG
                     DFDTVEEEIRQIKAACQDHILKVIVETCQLTKEELIELCGVVTRSGADFIKTSTGFST
                     AGATFEDVEVMAKYVGEGVKIKAAGGISSLEDAEKFIALGASRLGTSRIIKIVKNQKV
                     EEGTY"
     gene            9311..>9987
                     /locus_tag="VBC28_001641"
     CDS             9311..>9987
                     /locus_tag="VBC28_001641"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019770913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEO6815608.1"
                     /translation="MSQFDRIHLVVLDSVGIGAAPDANDFVNAGVPDGASDTLGHISK
                     TVGLAVPNMAKIGLGNIPRPQALKTVPAEENPSGYATKLQEVSLGKDTMTGHWEIMGL
                     NITEPFDTFWNGFPEDIITKIEDFSGRKVIREANKPYSGTAVIDDFGPRQMETGELII
                     YTSADPVLQIAAHEDIIPLEELYRICEYARSITMERPALLGRIIARPYVGEPGNFTRT
                     ANRHDYAV"
BASE COUNT         3151 a         1586 c         2148 g         3102 t
ORIGIN      
        1 aaatcaaatt taactattga caattatttt taaatgacgt aaacttatag gcaaccataa
       61 acaaggagag aataatatgt taatcaaatc gtctattatt aaacaaacaa atacttatta
      121 ttactttagt ttctttagat agacgtttgt agcctcaagg atacaaacgt cacgcaaagt
      181 ttgtatctat gaagctaaag cattttatga tgaaagcaga gccgattaga tcatttcaat
      241 aatctagttg gttaaggcaa tattttttcg ccctcccgtg cacagaggaa ttgagagaaa
      301 taattaacca actagatgta gggtctagtt ggttttttgt tttatggaaa atttgatggt
      361 attacaagga gatagtaatg aaaaaaataa caaagatggt aattagcagc gttttagcaa
      421 ctgttctgat gacagcttgt tcacaatctt ctcaaaatgg tcaatctgat gtaaaacata
      481 ttggtatttt acaatatgta gaacatccct ctctaactgc cactaggaaa ggatttataa
      541 aagaattagc taaagaaggc tataaagatg gcaaaaatat caaaattgag tataagaatg
      601 ctcaagggga tcagtcaaat atccaatcaa tttctgaaaa actcattaaa gataataaat
      661 tagtattggg aattgcgact ccggcggctc aatcgttgac aacggtgtca acagaaacac
      721 ctattttatt tacagcagtg acagatccgg tatcagccga attagtaaaa tcaatgaaaa
      781 aacctgaagg tttagcgact ggtacaagtg acatgtctcc aattaaaaag caagtatccc
      841 ttttgagaaa agtaatgcct aaggttaaac gtgttggtat tatgtatacc actagcgagc
      901 gtaactcaga agttcaggta aaacaagcaa aaaaaatctt ccaagaggcg gggattaaaa
      961 caagtgttaa aggtatttct tccactaatg atgtacaaga tactgctaag agtttaatgt
     1021 ctaaaacaga ggttattttt gtaccaacag ataatattat agccagctca gtaacgctgt
     1081 tagggaatct atcgaaagaa ttaaaagtgc ctgttgtggg aggttcagca gatatggtac
     1141 caagtggttt gctcttttct tatggggctg attatgaggc tttaggtcgt caaacggcaa
     1201 gacaagctgt taaaatcctc aaaggtaaag acgtcgctaa agttccttca gaatatcctc
     1261 aaaatctaaa agtagtagtt aatgaagata tggcaaaaga gcttggaatt gatgtttcaa
     1321 gtattaaaaa taataaataa tcagaaaggt attgtatgtt agatattatc ttatccggaa
     1381 tttcgcaagg attactttgg tcaattatgg caattggcgt gtttatcact tttcgtatct
     1441 tagacatagc cgatctctct gcagagggtg ctttccctat gggggctgca gtttgcgctt
     1501 tatgtatcgt taatgatatt aatccaattg tggcaaccat agcgggcatg ttaggtggta
     1561 tgttggcagg actagtatcg ggttttcttc atactaaaat gaaaatacca gccctcctca
     1621 caggtattat tacgttgaca ggactttact ctattaattt acttgtttta ggaagatcaa
     1681 acgtctcttt tgctctcaaa aatactttgg ttacaatggt aacaagatta ggtttaaata
     1741 aactttcagc agttttatta attggaattg tttgtgtagg cttggttatc ttaattttgt
     1801 atctcttttt aaatacccaa ctaggattag ctttaagagc gacaggtgat aatgaggcta
     1861 tgggacaagc caatagtatt aaagtagatc ggatgaaaat gctaggatat atgattggga
     1921 atggcctgat tgctttatct ggtgcacttt tagcgcaaaa taatggctat gcagatttga
     1981 atatgggagt gggtacaatc gttattggat tagcttcaat tatcttggca gaagtaatga
     2041 taaaatactt acctcttggc aaaaggttat ggtcaattgt attagggtct gttttatacc
     2101 gtatgattat tgttttcata ttaacaaccg atattgatgc acagatgata aagttggttt
     2161 cagcaatttt gttggctctc attttgtatg tcccagaatt acgagctaaa ctaggagtaa
     2221 aaacctctaa atcacttaca aaggaaggat aagaacatgt cattacttga attagtcaat
     2281 ctgcacaaaa cttttgaaaa aggaactgtc aatgagaacc atgttttacg tggtttagat
     2341 ttaacgattg aagacggtga ttttatttca gtaattggag gtaacggtgc gggaaaatca
     2401 accctcctta actgtatcgc tggtctgatc cctattgatc agggggcaat cacattagac
     2461 aaccaatcaa ttactaaaga ctctgtagaa aaacgatcta aggatattag tcgagtcttc
     2521 caggatccta gaatgggaac tgcaacaaat ttgacgattg aagaaaatat ggcaattgca
     2581 cataaaagag gcaataagcg tcacattttt agacagtcag tgactgacga tgaccgtcaa
     2641 ctatttaaaa agtctctatc acaattaggt ctaggcttag aaaatcgaat gaagactgat
     2701 gctgcatttt tatctggcgg tcagcgtcaa gccctcacgt tagctatggc aactcttgtt
     2761 cgtcccaaat tattattact ggatgaacat acagcggctc tagatcccaa aactagcgat
     2821 atggttatgg aattaacaca gaaagtgata gaagaacaac gattgacagc cttaatgatt
     2881 acgcacaata tggagcatgc cattgcttat ggtaatcgtc tcgtgatgct ctaccacggg
     2941 aaaattgttg ttgatgttaa aggagaagca aagagaaact taacagttgc agaattaatg
     3001 gaacttttcc ataaaaactc aggtcaacag ttgatagatg atgcccttgt cttaggttaa
     3061 aatataaatt tctctagtga atggacagga taactaggga aatttttctt gtttgaaacg
     3121 tgccttttct cttgactaat tctgaccaag tgatagaata gtttttgtag ttagcactct
     3181 ctgtatagga gtgctaaaaa atcaaaatat atctcgtatg gaggttcgtt tattatgtta
     3241 aaaccattag gtgatcgtgt gatcatttcg tttgttgaaa ctgaagagaa atctgtcggt
     3301 ggttttgttc ttgcaggagc aagccatgat gcgacaaaga cagccaaagt tcttgctgtg
     3361 ggtgacggta ttcgtacctt aacaggggaa ttagttgcac cgagcgttgc agagggtgat
     3421 actgttttag tcgaaaatgg tgctggtctg gaagttaaag acggtaacga aaaagtgaca
     3481 gttgttcgcg aaagtgacat tgttgctgtc gttaaatagg ttttattaag caagaaagga
     3541 tataggccta ttagagaaaa gttgctttgt aaagaaagta gactttgtta aattttaggc
     3601 ctgggtatta aattatggca aaagatatta aattttcagc agatgcccgc tcagcaatgg
     3661 tgcgtggtgt tgatatttta gctgatacag tcaaagtaac attaggtcct aaaggccgta
     3721 atgttgttct tgaaaaagca tttggttctc ctttaattac aaatgatggt gtgacaattg
     3781 ctaaagaaat tgagctagaa gatcactttg aaaatatggg agctaaactt gtgtcagaag
     3841 tggcttcaaa aactaatgat attgcagggg atggcactac aactgctact gttttgaccc
     3901 aagctattgt acgggaaggt cttaaaaatg taactgcagg ggcaaatccg attggcattc
     3961 gtcgtggtat tgaaacagct gtttcagcag cagttgaaga gctaaaagag attgcacaac
     4021 cagtttcagg caaagaagct attgctcaag ttgcggctgt gtcttcacgt tctgaaaaag
     4081 ttggggaata catttctgaa gctatggagc gcgtgggtaa tgatggtgtt atcactattg
     4141 aagaatcgcg aggtatggaa acagagcttg aagttgtgga aggaatgcag tttgaccgtg
     4201 ggtacttgtc acagtatatg gtaactgata acgagaaaat ggtctctgaa cttgagaatc
     4261 cgtatatcct tattacagat aagaaaattt caaatatcca agaaatttta ccattattag
     4321 aagaggttct taaaacaaat cgtccgttgc taatcatcgc tgatgatgtt gatggagaag
     4381 ctctcccaac gcttgttctt aacaaaattc gtggaacttt caatgtcgta gctgttaaag
     4441 cgcctggatt tggtgatcgt cgtaaagcca tgctggaaga tattgctatc ctaacaggag
     4501 gaactgtcgt tactgaagac cttggtttag acttaaaaga tgctactatg caagttttag
     4561 gacagtctgc taaagtaaca gtagataaag attctactgt tattgtcgaa ggtgccggtg
     4621 actcatcagc aattgctaat cgcgtagcta tcattaagtc acagatggag gctacaactt
     4681 ctgattttga tcgtgaaaaa ttacaagaac gacttgctaa gttagccggt ggtgtagcag
     4741 taattaaagt tggtgcagcg actgaaacag aattaaaaga gatgaaactt cgcatcgaag
     4801 atgcgttaaa tgcaacgcgt gctgcagttg aagaaggtat tgtttcaggt ggaggtacgg
     4861 ctcttgtgaa cgttattgaa aaagtagcgg cactgaaact taatggtgat gaggagactg
     4921 gacgtaatat tgttcttcgt gctctcgaag agcctgttcg tcaaattgct tacaatgctg
     4981 gatatgaagg ttcagttatt attgaacgtt taaaacagtc tgaaattggt acaggattta
     5041 atgcggccaa tggagaatgg gtagatatgg ttaccacagg tatcattgac cctgtcaaag
     5101 taacacgttc tgcacttcaa aatgcggcat ctgtagcaag tcttatcttg actacagaag
     5161 cagtagtagc aaataaacct gaaccagaag ctcctacagc tcctgcaatg gatccatcta
     5221 tgatgggtgg cttctaatca ctaacttatc acaacataaa actccccttt gacaaagggg
     5281 agttttatgt tgtgataata gagatatgat agtcactacc acttgcggta agaagatcat
     5341 aaggataggc catctaatat ttgccagtag tgggaggaaa aaagtagcaa aacaatgatt
     5401 gctattatct tagattgaga ttgataagct aagaattgaa ttatagctaa tagaattaag
     5461 taactaataa gtataaataa cagagaaaag agcattccgt acccagcacc cattgacggt
     5521 tcctttggag gggctagaaa ataacttaac ttaaaataag cgccaactat taggcccata
     5581 attaattcgg taaaagttat gaaaaaagca acttttaata gtctattttg cataggcttg
     5641 cctccttaat gagaggtaat ggacaattca aagtgctcaa tgggaacgta atgcttatta
     5701 cacattagta ttttctcttg cttaaaaatt gtttcttgta ttaatatgaa ttgtccatga
     5761 tcggataggg tatatttagt tgctgaaaaa ttgccagatg tcgcatatcc caaatggctt
     5821 tgtgagtggc ttgatatgcc ttcttgatag ataccttctt caatgaagtt taacaactgt
     5881 ttagtatcat gtaaagaaat ctcagcatct gaaataagtt cgatagggat aaaagaaagt
     5941 gtgtaagcta atggtccatc atcggtgtgg taccaacgat cagcaataac aactacaggg
     6001 gtttcttgtt taagtgaagc tgtgatagct tctgcaggaa cctctacacg aaattctaat
     6061 tctacttctg taattttgga agttagacaa gttttaatag ggtgttgtct attttcgtaa
     6121 ccgttttcag acaagttgct agaattttct ctaatgaaat taccttttcc tctgatattt
     6181 ttaaccaaat ggtcttcttg taatagtgct aatgactttc gtaaagtagc tcgactaaca
     6241 ttaagttgcc ctgctaattc gggttcagaa ggtaattgca ttccaggaga ataagtaccg
     6301 tcttgaatca tgttaaaaat cttattgtag acttgtacat gctttagttt atttagttta
     6361 ttatttagtt catttttagg cattgtaacc tccgtataca agttagttta attgaaaaaa
     6421 ttagaaaaat caagttaaaa aataaaacgt tttctttcga taaaagcgtt tacaaaggtt
     6481 gtcttgtatg ttattataca agtgtaaaaa aatagacaag acattaagga ggtctttatc
     6541 atgcagaatt attcaggtga agtcggattg caatatcacc tacagattcg tccaggtgat
     6601 gtaggtcgtt atgttatcat gccaggtgat ccaaaacgtt gtgcaaaaat tgcagaacat
     6661 tttgataatg cagttcttgt agcagacagc cgtgaatatg ttacttatac aggtacatta
     6721 aacggtgaaa aagtcagtgt tacttcaact ggtattggtg gtccatcagc gtctattgcg
     6781 atggaagaat tgaaactctg tggcgctgat acctttatcc gtgttggaac atgtggtggt
     6841 attgaccttg atgttaaagg cggagacatc gttattgcaa ctggagctat ccgtatggaa
     6901 ggtacaagta aagaatatgc tccgattgaa tttcctgctg tagctgactt ggaggtaacg
     6961 aatgctctag ttaatgcagc taaaaagctt ggttacacaa gtcacgcagg agtagttcaa
     7021 tgtaaagatg cattttatgg acaacatgag ccagaacgta tgccagttag ttatgaatta
     7081 ttaaataaat gggaagcatg gaaacgtctg ggaacaaaag cgtctgaaat ggaatctgca
     7141 gctctttttg tagcagcaag tcatctagga gttcgttgtg gatcagattt ccttgttgtt
     7201 ggtaatcaag aacgcaacgc tttgggtatg gataacccaa tggctcatga cacagaagca
     7261 gctattcaag ttgctgttga agcactccgc acacttattg aaaatgataa atcacaataa
     7321 gcgaggtttt ggaagtcatc atgcaattta tttatagtat tattggtatt ttattggtat
     7381 taggaattgt gtatgcaatt tctttcaatc gtaagagtgt ttctctaagt ttaattggaa
     7441 aagctcttat cgttcaattc attattgcgc taatcttagt acgtatccca ctaggtcaac
     7501 aagttgttag tgttgtttca actggagtta ctaaagtaat caactgtggt caagctggat
     7561 taaattttgt gtttggttca ttagcagata gtggagcaaa aactggtttt attttcgcca
     7621 ttcaaacgct tggcaatatt gttttcttat ctgccctagt tagtctactt tattatgtag
     7681 gaatccttgg atttgtagta aaatggatag gtaagggcgt tggtaaaatt atgaaatcct
     7741 cagaggttga gagttttgtt gccgtagcta atatgtttct tggtcaaaca gacagtccaa
     7801 ttttggttag caaataccta ggtcgtatga ctgatagtga gataatggtt gtgttggtat
     7861 caggtatggg aagtatgtca gtttctattc ttggtggcta cattgcatta ggcattccaa
     7921 tggaatatct cttgattgct tcaacaatgg ttcctattgg cagtattctc attgctaaaa
     7981 tcttattgcc tcaaacagaa cctgttcaaa aaattgatga cattaagatg gataataaag
     8041 gtaataacgc caatgtgatt gatgcaatcg ctgagggtgc aagcacaggt gcacaaatgg
     8101 ctttctcaat tggtgctagt ttgattgcct ttgttggttt agtttctttg attaatatga
     8161 tgttaagtgg attgggaatc cgcttagaac aaatcttctc atatgttttt gctccatttg
     8221 gttttcttat gggatttgac cacaaaaaca ttcttctaga aggaaacctt cttggaagta
     8281 agttgatttt aaatgagttt gtttcgttcc aacaattggg tgacctaatc aaatctttag
     8341 attatcgtac agcattggta gcaactattt cactttgtgg ttttgctaat ttatcaagtt
     8401 taggtatttg tgtttcaggt attgctgttc tttgtccaga gaaacgtggc accctagctc
     8461 gacttgtttt ccgtgcaatg attggtggta ttgctgtaag tatgcttagc gcctttatcg
     8521 tcggtattgt aactctattc taaaagttga caaaagaaag aaggatagta acgtggaagt
     8581 aaaagatatt ttaaaaacgg tagaccatac tttgctagca acaacagcaa catggccaga
     8641 aatccaaaca attttagatg atgcaatggc ttatgaaaca gcttcagcat gtattccagc
     8701 ctcttacgtc aaaaaagcag cagagtacgt ttcaggtaaa ttagctattt gtactgttat
     8761 tgggttccca aatggctata gtacaactgc agcgaaggtt tttgaatgtc aagatgctat
     8821 taaaaatggt gctgatgaaa ttgacatggt cattaatttg acagatgtta aaaatgggga
     8881 ttttgatact gttgaagaag aaattcgtca aatcaaggct gcatgtcaag accatatctt
     8941 aaaagttatc gttgagacat gtcaattaac taaagaagaa cttatcgaac tttgtggggt
     9001 tgtcacacgt tcaggtgcag actttattaa aacctctact ggtttttcga cagcaggtgc
     9061 tacatttgaa gatgttgaag tgatggcaaa atatgtcggc gaaggtgtta aaattaaggc
     9121 agcaggtgga atctcatcat tggaagatgc tgaaaaattt attgctttag gagcttcacg
     9181 cttgggtact agccgtatca tcaagattgt taagaaccaa aaagttgaag aaggcactta
     9241 ttaaataata taaaggaaat aggaaggatg ctggtctgac ttcctatttt caattgataa
     9301 aggagaagtt atgtcacaat ttgatcgcat tcacttagtt gtcttagatt cagtgggtat
     9361 tggagcggca ccagatgcca atgattttgt caatgcagga gttcctgatg gtgcttcaga
     9421 cacgcttggc cacatttcaa aaacagttgg acttgcggtg ccaaacatgg ctaaaattgg
     9481 acttggtaat attcctcgtc cccaagcgct taagaccgtc cctgcagaag aaaatccaag
     9541 tggctacgcg acgaagttac aagaagtatc acttggtaaa gatacaatga cagggcattg
     9601 ggagatcatg ggcttaaata ttacagagcc atttgatact ttctggaatg gttttccaga
     9661 agatattatc acaaagattg aggatttttc aggacgtaaa gttattcgtg aagcgaataa
     9721 accttattca gggacagctg ttattgatga ctttggacca cgccaaatgg aaactggtga
     9781 gctcattatc tatacctcag ctgacccagt cctacaaatt gcagctcatg aggatatcat
     9841 ccctctagaa gaactctacc ggatttgtga atatgctcgt agtatcacaa tggagcgtcc
     9901 agctctcctt ggtcgtatta tcgctcgtcc atatgtaggt gaaccaggta actttacgcg
     9961 tacagccaac cgtcacgact atgcagt
//
LOCUS       DATRMA010000071         9884 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.071, whole genome shotgun sequence.
ACCESSION   DATRMA010000071 DATRMA010000000
VERSION     DATRMA010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9884
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.071"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            <1..912
                     /locus_tag="VBC28_001642"
     CDS             <1..912
                     /locus_tag="VBC28_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000409543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 8 glycosyl transferase"
                     /protein_id="HEO6815609.1"
                     /translation="DSDIIVNTNLDYLFELDFKGYYLAAVKDPHKNEEGGFNAGMLLA
                     NLELWREDGLTKTLLKTAEELHRVVKTGDQSILNIVCHNRWLSLNKTWNFQTYDVVSR
                     YNHRSYLYLNIENRTPNIIHFLTSDKPWNENSVARFRELWWYYFQLDFCQLTGKQRKV
                     ISYEKSMELLSVSDIHLFTLTSSDNLEHIESLICRCPTVQFHIGAYTTVSNKLSKLEQ
                     YPNVLVYPELIEARIEKLITLATAYLDINHGPEVGNILQRVHLKQKPIYSFNNTSHQE
                     NITKHIVNHNSIDNMVVLINELNLNQL"
     gene            complement(959..1498)
                     /gene="nusG"
                     /locus_tag="VBC28_001643"
     CDS             complement(959..1498)
                     /gene="nusG"
                     /locus_tag="VBC28_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEO6815610.1"
                     /translation="MLDSFDKGWFVLQTYSGYENKVKENLLQRAQTYNMLDNILRVEI
                     PTQTVNVEKNGKTKEIEENRFPGYVLVEMVMTDEAWFVVRNTPNVTGFVGSHGNRSKP
                     TPLLEEEIRSILISMGQTVDVFDTNIKEGDVVQIIDGAFIGQEGRVVEIENNKVKLMI
                     NMFGSETQAELELYQVAEL"
     gene            complement(1704..3911)
                     /locus_tag="VBC28_001644"
                     /pseudo
     CDS             complement(1704..3911)
                     /locus_tag="VBC28_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063250.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif.; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
     gene            complement(4223..4396)
                     /gene="secE"
                     /locus_tag="VBC28_001645"
     CDS             complement(4223..4396)
                     /gene="secE"
                     /locus_tag="VBC28_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEO6815611.1"
                     /translation="MFVKGIFQVLRDTTWPNRKQRWKDFISILEYTVFFTIVIYIFDK
                     LLAAGVMDLINRF"
     gene            complement(4432..4584)
                     /gene="rpmG"
                     /locus_tag="VBC28_001646"
     CDS             complement(4432..4584)
                     /gene="rpmG"
                     /locus_tag="VBC28_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEO6815612.1"
                     /translation="MAQKKASLACTECGSRNYSIGVSSTPKPTRLEVNKFCKYCKKYT
                     IHKETR"
     gene            complement(4633..6954)
                     /locus_tag="VBC28_001647"
     CDS             complement(4633..6954)
                     /locus_tag="VBC28_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000764805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEO6815613.1"
                     /translation="MKLFDKFIDLFRVDEDNDEMTRKNEQETREETSNLDGEEVYDID
                     DITRPSKSQYQRGIRHQKENAKSRPEWLQKVDRYLPSPKNPIRRFWRRYRIGKLLFIA
                     LMAFILIFGSYLFYLSKTATVSDLQSALKTTTTIYDKNKEYAGKLSGQKGTYVELNAI
                     SDHLKNAVIATEDRTFYENNGVNFKRFFLAVATLGKFGGGSTITQQLAKNAYLSQDQT
                     IKRKAREFFLALELTKKYSKAEILTMYFNNSYFGNGVWGVEDASRKYFGTSAANLTVD
                     EAATLAGMLKGPEVYNPYYSVENATNRRDTVLAAMVDAGKLTKSQAKEAASIGMKNRL
                     ADTYAGKINDYRYPSYFDAVVNEAIDTYGISEKDIVNNGYKIYTALDQNYQSGMQKTF
                     DDTSLFPVSDYDGQSAQGASVALDPKTGGVRGLVGRVQSTKDAQFRSFNYATQSKRSP
                     ASTIKPLVVYSPAIASGWSIDKELPNKVQDFHGYKPSNYGGIETESIPMYQALANSYN
                     IPAVYTLDKLGINKAFTYGRKFGLNMSSANKELGVALGGSVTTNPLEMAQAYSTFAND
                     GIMHRAHLITRIETANGKLVKQFTDKPKRVISRSVASKMTSMMLGTFSNGTAINANVY
                     GYTMAGKTGTTETDFNPNLSGDQWVVGYTPDVVISQWVGFKNTDKHHYLTDSSAGTAS
                     NIFSTQASYILPYTKGSSFTHIENAYFQNGIGSVYNAQDASNTTNQESRSIINDLKDS
                     ASKAAQDISRAVEDSNFQEKVKDAWNSLKDYFR"
     gene            6998..7882
                     /locus_tag="VBC28_001648"
     CDS             6998..7882
                     /locus_tag="VBC28_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEO6815614.1"
                     /translation="MSFSLKIRNPYGEHTVKELLEDYFLIPRKIRHFLRVKKHVLINN
                     EFINWQTVVQENDTITLIFDDEDYPTKKIPLGRAELIDCLYEDEHLIIVNKPEGMKTH
                     GNQPNEIALLNHVSAYSGQTCYVVHRLDMETSGAVLFAKNPFILPLINQRLERKEIWR
                     EYWALVEGKFSPKHQVLRDKIGRNRHDRRKRIIDSKNGQHAMTIIDVLKYIQNSSLIK
                     CRLETGRTHQIRVHLSHHGHPLIGDPLYNPSSNNERLMLHAHRLTLSHPLTCETISVE
                     APSSTFEKILNNYKKGVG"
     gene            complement(7907..9547)
                     /locus_tag="VBC28_001649"
     CDS             complement(7907..9547)
                     /locus_tag="VBC28_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000459235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6815615.1"
                     /translation="MFDFFKKKVVKVCLVIFGIVLVSLLSLGFFYFSKGQVLSRFVAA
                     RSRTSGQAFDNIKEYMVWSDTGESITNDEANYANFEPLSKSEARKLGQEIKEGNKNDS
                     MYLKRVGSRLGIFPDYRIANKPMSLTLKTNVPKLDVLLNQKKVATSNSDHFSVTVERL
                     PRTHYTASLEGTSDGKEIKLKKDYDGKNQTIDLSVAFKSFTVTSNLMDGNLYFGDNRI
                     AKLKDGSHSVENYPVTDGSKAYIKKVFNDGEITSHKQKLISIADNQTIKLDVDGLLNE
                     KEAGQKLITAFNQLILYVSTGQDPQTLGTVFEKGAENDFYKGLKESIKAKFVTDNRKA
                     SHFTIPNIVLNKMTQVGKESYQVNFAADYDFNYDKSTDPDKKTYGHIIQNLTGNFIMK
                     KSGNSYLISNDGKKDITVAKETNKVKADPVSIFPENLVGSWKGEVEDGTVTMTFDKDG
                     KVTQKKVYKDSKSKESNHSAKVTKLEDKGNGLYLYQYESGTDTTTFVTGGIGGLKVKY
                     AYGIKIEGNKIIPVIWQTSSDGEFDYHKPLLSKPLTKQ"
     gene            complement(9717..>9884)
                     /locus_tag="VBC28_001650"
     CDS             complement(9717..>9884)
                     /locus_tag="VBC28_001650"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019318858.1"
                     /note="catalyzes the transfer of phosphate between the C1
                     and C5 carbons of pentose; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEO6815616.1"
                     /translation="TDHTREYIPLLAYSPSFTGNGLIPVGHFADISATVADNFGVDTA
                     MIGESFLQDLV"
BASE COUNT         2936 a         1871 c         1552 g         3525 t
ORIGIN      
        1 gattcagata ttatcgttaa cactaatctt gattacttat ttgaactaga ttttaaaggt
       61 tattacttag cagccgtcaa agatccccat aaaaatgaag aaggagggtt taatgctggc
      121 atgcttttag ctaatctaga actatggcgg gaagatgggc tcactaaaac attactaaaa
      181 acagctgaag aactccaccg agttgtcaaa acaggggatc aaagtatctt gaacattgtt
      241 tgccataatc gttggttatc tctgaacaaa acatggaact ttcaaactta tgatgtcgtt
      301 agccgctata atcatcgatc ttatttatat ctaaacatag aaaatagaac tcctaatatt
      361 atacattttt taactagtga caaaccttgg aatgaaaata gcgttgcaag gtttagagaa
      421 ctatggtggt attacttcca acttgatttt tgccaattaa ccggcaagca aagaaaagtg
      481 atttcttacg aaaagtccat ggaattgctt tctgtttcag atattcatct tttcactctt
      541 acatcttccg ataatttaga acacattgaa tcgctaattt gtagatgtcc tactgttcaa
      601 ttccatattg gtgcctacac aacagtgtca aataaactta gcaaactaga acaatatcca
      661 aatgtcctag tttaccctga attaattgag gcaagaattg aaaaattaat aacattagcc
      721 actgcatacc ttgatattaa tcatggtcca gaagtaggta atattttaca aagagttcat
      781 ttgaaacaaa agccaattta ttctttcaac aacacgtctc atcaagagaa tataaccaaa
      841 catatcgtta atcataattc tattgataat atggttgttt taattaacga attaaaccta
      901 aatcaattat aaattaacaa aaatccatct ttattttaag atggattttt cagaagtttt
      961 ataattctgc aacttggtac aactcaagtt ctgcttgtgt ttctgagccg aacatgttga
     1021 tcattaattt aactttgtta ttctcaatct caactacacg tccttcttgt ccgatgaaag
     1081 caccatcaat gatttgaaca acatcacctt ccttgatatt agtatcaaag acatcaacgg
     1141 tttgtcccat tgagatgagt attgaacgaa tctcttcttc taacaacggt gttggtttag
     1201 agcgattgcc gtgtgatcca acaaatccag taacgtttgg agtattacga acaacaaacc
     1261 aggcttcatc agtcattacc atttccacaa gtacataacc cgggaagcga ttttcttcaa
     1321 tttcttttgt tttcccattt ttctcaacat ttacagtttg agtagggatt tccacacgca
     1381 aaatgttgtc taacatatta taagtttgcg cacgttgcaa gagattttcc tttactttat
     1441 tttcataacc tgagtaggtt tggagtacaa accaaccctt atcaaaagaa tctaacataa
     1501 gttttccttt ccttaataaa aaagcctttt acggcttatt ggactgctat tttcaatata
     1561 tattataaag aaaataaaat aagctgtcaa ttaaagtacc gactaaatca ttctaaaatt
     1621 caaataaaaa aggcaaagtt ctgatgaggt ttgccttaag attttgaaag gatagacctt
     1681 tcggtttatg tttaaaaata aatttaattt tttttatgct ttaaaactat cgtaataaga
     1741 cctgcactta acataacaat tagtgataca attgctaaga gtggacttgc ggcttcaccc
     1801 gttgatggta attttttact atactttttg ttttcaatag cttttttagc cgccaagttt
     1861 ccgctagtat taaccgattt tttggttgct ggtttggcct ctggtttagc ttctagctta
     1921 gcctctggtt tagcttctga cttagcctct ggtttagctt ctggcttagc ctctggttta
     1981 acgtctggtt tagcttctgg cttaacgtct ggcttagctt ctggtttggc ctctggctta
     2041 gcttctggct tagcttctgg cttagcttct ggtttggcct ctggcttaac gtctggttta
     2101 gcttctggct taacgtctgg tttaacgtct ggcttatcct ctggtttggc ctctggctta
     2161 acgtctggtt taacgtctgg tttaacgtct ggcttagctt ctggcttaac gtctggtttg
     2221 gcctttggtt taacgtctgg cttagcttct ggtttaacgt ctggcttagc ttctggttta
     2281 acgtctggct tagcttctgg tttaacgtct ggcttagctt ctggcttaac gtctggtttg
     2341 gcctctggct taacgtctgg tttggcctct ggtttggctt ctggtttggc tactttttta
     2401 tcttttaatt ctaaactttg taaagcatca cgtagcttgt tagctaattg atttgcttga
     2461 tcagtggtaa taaattttcc ctcttccaca tgatcataag cttggtcaac agctttttga
     2521 actgcaagta tatctacgta tttgtccttt acttgatcat ctttaagcag atctttagct
     2581 tgattatacc atgtagtcaa tcttgtcaag gcttctggtg acaaagctgg ttttgcagct
     2641 tctgtaggtg cttgtttagg tacttgaata tttggtttgg cctctggttt ggcttctggc
     2701 ttaacgtctg gttttttatc ttctttagta cctctggtaa ggtcttgaag ttcttttaaa
     2761 agctttttga cttcatcaag catttttttt gcttcatcaa gtccactttg accagtccaa
     2821 acactcctat ttccgaaagt ttgtttatct ctaaacctat tcaaataagt tcccacagag
     2881 tctgcataat atccaggtgt aggcaaaata cttttatgct tttcataaat ttctttagcc
     2941 tttttagata acgctaatcc ttttgaactt attctttcaa tttttttagc atactgcttt
     3001 tgtaagtttt tctgggcaac ttcctgtgct gcttgcttaa tactatttaa agcattcatt
     3061 gcagcagtaa tattgagctt accttcatta tactgtttca ctacattatc aattgcagta
     3121 ttatatctca acatttcatc gttagataaa ttttctaact tagcaatttc ttgcttttta
     3181 ccattggcct gatttaccaa cgtttcttta ttggaattaa caactttttt agcttctgct
     3241 acaaactcat caacttgact cttagtgcta gctctttgaa ttttttcttt atattcttct
     3301 aagacttcgg ggcttatatt ttttattaag gccacgtcat tttgagccat ttgcttagct
     3361 tttgttgatt cagcattatt cagttgcgtg gcttgagtta caaatttaag aataagttgc
     3421 gaatcttttg aagagctaat agatttaact aaaatatctt tttggtctta tgaaaggaaa
     3481 cctaatgact taatgatatc ttgagaacgt cttgactgct tacctagttc ttgatcacta
     3541 actttagcat gtgtagttac acttggttta ttttgcttac caatttgtcc ttgatataaa
     3601 atatttctaa tttctgcttc agtactatta attaattgtg gaagtgtatc ctgtgatgct
     3661 ctattaaaat catttactct acttaaaagt atagcaatag ctttgtcatt accacgatat
     3721 ttagaaatca aattcttcag agtaaccgcc cctaaattaa cttgtttctt atgaggaatt
     3781 gaagccgata ttcctgaact agtatccgcg tgtacaatac cactacatac agcaaacgct
     3841 gctgacattg aggccaaacc ataagctgtt tttcttaaaa agtattttac ttttttttcg
     3901 ttattattca tattcgctcc tttatatagt tagttgaaaa tcctaattta ttaggatagg
     3961 accacgttta ttttaattta atttgagttc cttaactaat tatataactt ggagtttttt
     4021 atttcaacac ttttctttaa tttatttaca ttttattaaa ataaacatct tttttttaat
     4081 gtttttttgc tcaacaatct agtaaaaata atcaaaagat tattttttta taatttaatc
     4141 ggatatatat gttattttcc acacaaaaaa cccttagtaa aatctaaggg tttgggttgt
     4201 ttctatctaa aataatctat tattaaaatc gattaatcaa gtccatgact cctgcggcta
     4261 acagtttgtc aaagatgtag ataacgattg tgaaaaatac agtgtattcc aagatggaaa
     4321 taaaatcttt ccaacgttgt tttcgatttg gccaagtagt gtcacgcaat acttgaaaaa
     4381 tccctttgac aaacattatc agttctcctt tttgcagttt gttacgctat atcaacgcgt
     4441 ttctttgtgt attgtatact tcttacaata tttacaaaat ttatttactt ctagtcgtgt
     4501 tggcttgggg gtgctactta ctccgattga gtaatttcgg cttccgcatt ctgtacacgc
     4561 taggcttgct ttcttttgtg ccataacgcc tccataagtg ctattttaac atgatttgta
     4621 taatcctgca agctatctaa agtagtcctt tagactattc caagcatctt ttaccttctc
     4681 ttggaaattt gaatcttcca ccgctctaga aatatcttgt gcagccttac ttgccgaatc
     4741 ttttaaatca ttaataattg agcgtgattc ttggttcgtt gtattactag catcttgtgc
     4801 attataaact gaaccaatac cgttttggaa ataagcattc tcaatatgag taaaactaga
     4861 ccccttagta tacggtagta tatatgaagc ttgcgtgcta aatatatttg aagctgtacc
     4921 tgcactagaa tctgtcaaat aatggtgttt atctgtgttc ttaaagccaa cccattgact
     4981 aataacgaca tcaggcgtat agccgactac ccactggtct ccagataaat tggggttaaa
     5041 atctgtttcg gttgttccag ttttacctgc cattgtataa ccataaacat tggcattaat
     5101 agctgtacca tttgagaatg ttcctaacat catactagtc attttactag ccactgagcg
     5161 actaataact cgcttaggct tatctgtaaa ttgctttact aattttccat tagctgtctc
     5221 aattcgagta atcaaatgag cgcgatgcat tataccgtca tttgcaaaag ttgagtaagc
     5281 ttgtgccatt tctaaaggat tagtggttac acttccacct agcgccactc ctagctcttt
     5341 gtttgctgat gacatattta gaccaaactt cctgccataa gtaaaggctt tattgattcc
     5401 tagtttgtct aaagtataaa cagcaggaat attataagaa ttagctagtg cttgatacat
     5461 cgggatactt tcagtctcaa tcccaccata gttacttggt ttataaccat gaaaatcttg
     5521 aactttatta ggaagttctt tatctattga ccatcctgaa gcaatagctg gtgaataaac
     5581 aactaaaggt tttatggttg aggctggact tctctttgat tgagtcgcgt agttaaaact
     5641 tctaaactgt gcatctttag tactttgaac acgtccaact aaacctctta caccaccagt
     5701 ttttggatct aaagctacgc tagcaccttg ggcagattga ccatcatagt cagatacagg
     5761 gaagaggcta gtatcatcaa atgttttttg cattcctgat tgataatttt gatctaatgc
     5821 agtgtaaatc ttatagccat tattgacaat atctttttct gaaataccat aagtatcaat
     5881 ggcttcattg acaacagcat caaaatagga tgggtacctg taatcattaa ttttaccagc
     5941 ataggtatct gctaatctat ttttcatacc aattgaagca gcttctttag cttgagactt
     6001 ggttagttta cctgcatcaa ccatcgctgc caaaacagta tctcttcggt tagttgcatt
     6061 ttcaacagaa taataaggat tataaacttc tggtccttta agcattccag caagtgtagc
     6121 agcctcatct actgtcaaat tagcagcact ggttccaaaa tatttcctac tagcatcttc
     6181 cacaccccaa acaccattac caaaataaga gttattaaaa tacatagtca gaatctcagc
     6241 cttgctatat ttcttagtca attctagcgc taaaaagaat tcacgcgcct tgcgtttaat
     6301 cgtttggtcc tgtgacaagt aagcattttt tgctaattgc tgggtaatag ttgagcctcc
     6361 gccaaatttc ccaagtgtag ctactgccaa aaaaaatcgc ttaaagttta caccattatt
     6421 ttcataaaaa gttctatctt cagttgctat aacagcattt ttgagatgat cagaaatagc
     6481 atttaactct acataagttc ccttttgtcc cgaaagtttt ccagcatatt ctttattctt
     6541 atcataaatg gtggtggttg tttttagagc actctgtaaa tcactaactg tagcagtttt
     6601 agaaagataa aaaagatagc taccaaatat aagaataaaa gccattaagg cgataaaaag
     6661 caacttccca atacgatagc gccgccaaaa acgacggata ggatttttag gagaagggag
     6721 gtaacgatca actttttgta gccattcagg tcgagattta gcattttcct tttggtgacg
     6781 aatgcctcgc tgatactggg atttactagg tcgggtaata tcatcaatat catagacttc
     6841 ttcgccatcc aaattgctag tttcttctct agtttcttgt tcattttttc ttgtcatttc
     6901 gtcgttgtct tcatcaacac gaaataagtc tataaactta tcaaataatt tcatagactt
     6961 attatatcaa ttttcaataa aatgctataa taaaaccatg tcattttcat taaaaattag
     7021 aaatccatac ggtgaacata ccgttaaaga actccttgaa gattattttt tgattccacg
     7081 taagattaga cattttttgc gtgttaaaaa acatgtactt ataaacaatg aatttattaa
     7141 ttggcaaact gtcgtccaag aaaacgatac tattacctta atctttgatg atgaggatta
     7201 ccctactaaa aaaattcctc tgggcagagc agagcttatt gattgtcttt atgaggatga
     7261 acatcttatt atcgttaata aacctgaagg tatgaaaact cacggtaacc aaccaaatga
     7321 aatagcactg ttaaatcatg tatctgccta ttctggacaa acatgctatg ttgttcatcg
     7381 cctagatatg gagaccagtg gagctgtttt atttgctaaa aatccattta tacttcccct
     7441 tatcaatcaa cgcttagaac gaaaagaaat ttggcgtgaa tattgggctt tagttgaagg
     7501 aaaattttca cctaagcatc aagttttgag agacaaaatt ggacggaacc gtcatgacag
     7561 acgtaaacga atcattgatt ctaaaaacgg tcaacatgct atgacaatca ttgacgtttt
     7621 gaagtatatc caaaatagta gtctcataaa atgccgactg gaaaccggaa gaacccatca
     7681 aattcgcgtt cacttatctc atcatggaca tcccttaata ggagaccccc tctacaaccc
     7741 ttcttctaat aatgaaaggt taatgctaca cgctcaccga ttgactctat ctcatccatt
     7801 aacttgcgaa actattagcg tagaggcccc ttcatctact ttcgagaaga ttttaaacaa
     7861 ttataaaaaa ggagttggat aataattatc caactccttt taatcattat tgcttagtta
     7921 atggcttact gagtagtggt ttatggtaat caaattctcc atcactagat gtctgccaga
     7981 tcactgggat gatcttattc ccttcaattt ttattccata ggcgtattta actttaagac
     8041 caccgatacc tcctgttaca aaagttgtag tatcagtacc tgattcatat tgataaaggt
     8101 agagcccatt tcccttatcc tccaacttcg taactttagc agaatggtta ctttccttag
     8161 atttactatc cttgtaaact tttttctgag taactttacc atccttatca aaagtcatcg
     8221 ttactgtacc gtcctcaact tcacctttcc aacttcctac aaggttctct ggaaaaatgg
     8281 aaacgggatc agccttaact ttattagttt ccttagctac tgtgatatct tttttaccat
     8341 cattagaaat aaggtaacta tttcccgatt ttttcataat aaaattacca gttaaattct
     8401 gaataatgtg gccatatgtt ttcttatcag gatctgttga tttatcataa ttaaaatcat
     8461 aatcggcagc aaaattaact tgataacttt ctttcccaac ctgtgtcatt ttattaagga
     8521 caatattagg aatagtgaaa tggctcgctt tacgattatc agttacaaat tttgctttaa
     8581 tactttcttt caatcctttg taaaaatcat tttcggcacc tttttcaaaa actgttccta
     8641 aagtctgagg gtcttgccca gttgatacat aaagtatcaa ttgattgaaa gcagttatca
     8701 atttctgtcc agcttctttt tcatttaata aaccatcaac atctaatttg attgtctgat
     8761 tatcagcgat agagattaat ttttgtttat gagaggttat ctcaccatca ttaaaaactt
     8821 ttttgatata agcttttgaa ccatcagtca ctggataatt ctctacggaa tggctaccat
     8881 cttttaattt agcaatacga ttatcaccaa aataaagatt accatccata agattacttg
     8941 ttactgtaaa agatttaaaa gcgaccgata aatcaatagt ttggttttta ccatcataat
     9001 cttttttgag tttaatctct tttccgtcac tagtcccttc aaggctagca gtataatggg
     9061 ttctaggtaa acgttccacc gttactgaaa aatgatctga attgcttgtc gcgacctttt
     9121 tttgattcaa caaaacatcc aatttaggaa cattggtttt caaggtcaaa gacataggct
     9181 tgttagcaat acgataatca gggaatatac ctaagcgact acctacacgc tttagataca
     9241 ttgaatcatt tttgtttcct tcttttattt cctgccctaa tttacgcgcc tcacttttag
     9301 acaagggctc aaaattagcg taattggcct catcatttgt gatagattct cccgtatctg
     9361 accaaaccat gtattcttta atattatcaa acgcttgacc tgacgtacga gaacgtgctg
     9421 ctacaaaacg actaagtact tgtccctttg aaaaataaaa gaatcctaga ctcagaagtg
     9481 atactaaaac tataccaaaa ataactaagc acactttaac aacttttttc ttaaaaaaat
     9541 caaacatacc ttatcctctc attcacttta tattaaagta aattgcttaa agaaaagtta
     9601 caacactgat aaaacagctg tatttgtact tgtaaccgct atttttctat tataacaaaa
     9661 cgaaaaaagg attgaaacca taatccgatt tcaacctcta aaatttttgt ctatgattat
     9721 actaagtcct gcaagaagct ttctccaatc atcgcagtat caacaccaaa gttatccgca
     9781 actgttgctg aaatatctgc gaaatgccct actgggataa gtccgtttcc tgtgaaggat
     9841 gggctatagg ctaagagagg aatgtattct cttgtatggt ctgt
//
LOCUS       DATRMA010000072         9882 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.072, whole genome shotgun sequence.
ACCESSION   DATRMA010000072 DATRMA010000000
VERSION     DATRMA010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9882
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.072"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            313..1482
                     /locus_tag="VBC28_001651"
     CDS             313..1482
                     /locus_tag="VBC28_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEO6815617.1"
                     /translation="MAWLGNFFTGASFSLVMPFMVLYVEQLGAPSNKVEWYAGLSVSL
                     SALSSALVAPLWGRLADKYGRKPMMVRAGLMMTFTMGGLAFIHSVTGLLILRILNGIF
                     AGYVPNSTALIASQAPQEESGYALGTLATGVTGGMLIGPLLGGLLAEWFGIREVFLLV
                     GTILLISTLMTIFMVKEDFKPISNEETMPTTEVFKSVKSLQILIGLFVTSMIIQISAQ
                     SIAPILTLYIRHLGQTENLMFVSGLIVSGMGFSSILSSPKLGRIGDRIGNHRLLLLAL
                     LYSFLMYVLCSLAQTSLQLGVIRFLYGFGTGALMPSINSILTKIAPRQGLSRIFSYNQ
                     MFSNLGQVLGPFVGSAVSIHLGFRWVFFVTSFIVLANFVWCFINFRKYIRVKEIV"
     gene            1479..1625
                     /gene="rpmG"
                     /locus_tag="VBC28_001652"
     CDS             1479..1625
                     /gene="rpmG"
                     /locus_tag="VBC28_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEO6815618.1"
                     /translation="MRVKINLKCSSCGSKNYLTSKNKSNHPDKIEVPKYCPKERKVTL
                     HIES"
     gene            1672..1908
                     /gene="secG"
                     /locus_tag="VBC28_001653"
     CDS             1672..1908
                     /gene="secG"
                     /locus_tag="VBC28_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEO6815619.1"
                     /translation="MYNLLLTILLVLSVLLIISIFMQPQKNPSSNVFDSSGSEALFER
                     SKARGFEAFMQRFTGVLVFFWLLIGLVLSILSSH"
     gene            2021..4426
                     /gene="rnr"
                     /locus_tag="VBC28_001654"
     CDS             2021..4426
                     /gene="rnr"
                     /locus_tag="VBC28_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEO6815620.1"
                     /translation="MKEKIIKYLEEHGKTSVNDLAAALDMAGAKAFPKLIKTISNLES
                     HRQLRFDDNGSLSLQKKEAKKKEITVRGLFRANKAGFGFLSIDQDEDDMFIGKNDIAY
                     AIDGDTVEAVVKKPADRLNGTAAEVRVVNIVERSLKTLVGKFVLDDERPKYAGYIKSK
                     NQKINQKIYIRKEPVVLDGTEIIKVDIDKYPTRGHDYFVASVRDIVGHQGDVGIDVLE
                     VLESMDIVSEFPEDVIAEANAIPDAPTEKDLIGRVDLRQEVTFTIDGADAKDLDDAVH
                     IKLLDNGHFELGVHIADVSYYVTEGSALNREALSRGTSVYVTDRVVPMLPERLSNGIC
                     SLNPNLDRLTQSCIMEIDQNGRVVNHQITQSVINTTYRMTYTAVNDIIAGDEEICSEY
                     ESIVSSVQHMVTLHHTLEAMRTRRGALNFDTSEAKIMVNDKGMPVDIVIRNRGIAERM
                     IESFMLAANETVAEHYARLKLPFIYRIHEEPKAEKLQKFIDYASVFGVQIQGTATKIT
                     QSALQDFMKKVQGQPGSEVLSMMLLRSMQQARYSEHNHGHYGLAAEYYTHFTSPIRRY
                     PDLLVHRMIRDYDDKAMDKADHFANLIPEIATQTSSLERRAIDAERIVEAMKKAEYME
                     EYVGEEFEGVVASVVKFGMFVELPNTIEGLIHVTTLPEYYHFNERTLTLQGEKSGKVF
                     RVGQQIKVKLIRSDKETGDIDFDYLPSDFDIVEKVSKSSREGRPNRSSKREHQHRISD
                     RDNKNKNTSKKKASRKPKRNSDSKSHHHKDDRTTGSTKKKTKKPFYKGVAKKGQKRKS
                     KKG"
     gene            4429..4896
                     /gene="smpB"
                     /locus_tag="VBC28_001655"
     CDS             4429..4896
                     /gene="smpB"
                     /locus_tag="VBC28_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEO6815621.1"
                     /translation="MVKGQGNVVAQNKKAHHDYTIVETIEAGIVLTGTEIKSVRAARI
                     TLKDGYAQIKNGEAWLINVHITPYDQGNIWNQDPDRTRKLLLKKREIEKISNELKGTG
                     MTLVPLKVYLKDGFAKVLLGLAKGKHDYDKRESIKRREQNRDIARQLKNYNSR"
     gene            complement(4980..6368)
                     /locus_tag="VBC28_001656"
     CDS             complement(4980..6368)
                     /locus_tag="VBC28_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000042127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEO6815622.1"
                     /translation="MSKNNNDHTQKSENGMVRGLENRHVQLIAIAGTIGTGLFLGAGR
                     SISLTGPSIVLVYAITGAFMFFMMRAIGEMLYQDPNQHTFINFITKYLGPGWGFFSTL
                     SYWISLIFIGMAEITAVGEYVQFWFPEWPSWIIQIVFLAILSSINLIAVKAFGETEFW
                     FAMIKVIAILGLIATGIFMVLTNFDTGHGYHASISNITNHFEWFPKGKLNFFMAFQMV
                     FFAYLAIEFVGVTTSETANPRKVLPKAIQEIPMRIILFYAGSLLAIMAIFPWQQLPVN
                     ESPFVTVFKLAGIKWAAALINFVVLTSAASALNSTLYSTGRHLFQLANESPNALTKAL
                     KLDQLSRQSVPSRAIIASAVIVGASALISVLPGISDAFSLITASSSGVYISIYVLIMI
                     AHWKYRKSPDFMEDGYKMPAYKILSPITLLFFLFVFVSLFLQDSTYIGAIGATIWIIG
                     FGLYSHFKHKSH"
     gene            complement(6497..7423)
                     /locus_tag="VBC28_001657"
     CDS             complement(6497..7423)
                     /locus_tag="VBC28_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEO6815623.1"
                     /translation="MALLSVIVPCYNEQETVSTFLTEIKKVESEMARYTHFEYIFVND
                     GSTDRTLELLKKAAKQFDNVHYLSFSRHFGKDAALLAGLEHTTGDFITVMDVDLQDPP
                     TLLPEMYLKLQEGYDIVATRRKDRKGEPLIRSLFAKLFYKLINQVSDTKMVDGARDFR
                     LMTKQVVDSILELNEVNRFSKGIFSWIGYDVAYISYENRERIAGKTSWSFFNLLKYSL
                     DGFINFSEIPLAIATWIGTLSSVLSLLAIIFIIIRKLLFGDPVSGWASTVTIVLFMGG
                     IQLLSLGIIGKYISKIFLETKKRPVYIVKEEG"
     gene            complement(7552..7944)
                     /gene="gloA"
                     /locus_tag="VBC28_001658"
     CDS             complement(7552..7944)
                     /gene="gloA"
                     /locus_tag="VBC28_001658"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HEO6815624.1"
                     /translation="MPFLHTCIRVKDLDASIAFYQEALGFKEVRRNDFPENQFTLVYM
                     ALEDDPSYELELTYNYDHEAYDLGNGYGHIAVGVDDLETTYDAHQKAGYSVTKISGLP
                     GKPNMFYFIQDPDGYKIEVIRLSQFKAD"
     gene            complement(8032..8706)
                     /locus_tag="VBC28_001659"
     CDS             complement(8032..8706)
                     /locus_tag="VBC28_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000159250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEO6815625.1"
                     /translation="MTKEDIKKQVRRAFDFRMAIRVYNNNDIPKEDMEYILDTAWLSP
                     SSVGLEGWRFLVLDRQTIAKFRDKLKEVAWGAQYQLDTASHFVLLLAEKGAYYNADSM
                     INSLIRRGLGDPAALESRIPLYKSFQENDMKIDSERSLWDWTAKQTYIALGNMMTAAA
                     MIGVDSCPIEGFDYEKVNNILSKEGLIDDKKEAISCMVSFGYRLREPKHSRARKERQE
                     VITWVE"
     gene            8973..9815
                     /locus_tag="VBC28_001660"
     CDS             8973..9815
                     /locus_tag="VBC28_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEO6815626.1"
                     /translation="METYTLSNTLNIPKIGFGTWQLTEGEEAYKAVTHALKVGYTHID
                     TAQIYGNEHSVGRAIRDSGLARESIFLTTKIWNDKHDYHLAKASIDESLQKLGVDYID
                     LLLIHWPNPKALRENDAWKAGNAGTWKAMEEAYKEGKVKAIGVSNFMKHHLEALFETA
                     EIKPMVNQIILAPGCAQEDLVRFCKGNDILLEAYSPFGTGAIFENESIKAIAEKYGKS
                     VAQVALRWSLDNGFLPLPKSATPKNIEANLDIFDFQLNEDDIATLIQLDSGIKPKDPD
                     NVSF"
BASE COUNT         3328 a         1598 c         1910 g         3046 t
ORIGIN      
        1 aaatctataa cgatgccaat attattagta agcctaggaa ttatagttgt tattttaatt
       61 attggctatt tagcagttgt agtatttgct ccaaaaactt tccgtttaaa aagttaattt
      121 taattgtggt ataataacag aggcatgtga taacataggt ttagttacat aaaggaaatt
      181 ataatggaaa taacatctca aatagatgtt aaatctcaat aaaactgcta agatagttag
      241 tggtagaaaa gtttagaaag aagatgatgt tttatctcag ttgaaacggt taactggaag
      301 caaaatttaa aagtggcttg gctcggtaat ttttttactg gggcaagctt ttcacttgtt
      361 atgcctttta tggtattata tgtagaacaa ttaggagcac ctagcaacaa agtagagtgg
      421 tatgcaggcc tatcagtatc cttatcagcc ttgtcttcag ctttagttgc cccattatgg
      481 gggcgcttag ctgataaata tggtagaaaa ccgatgatgg taagagcagg gttgatgatg
      541 acttttacta tgggaggtct tgctttcatt catagtgtta caggacttct gattttacgt
      601 atattaaacg gcatttttgc aggctatgta cctaactcta cagctttgat agcaagtcaa
      661 gctcctcaag aggaatcagg atatgcctta ggaaccttag cgacaggagt gactggaggt
      721 atgttaattg gtccactttt agggggactt ttagcagagt ggtttggtat tagggaagtt
      781 tttttattag tgggaaccat attgttaata tctactttaa tgaccatttt tatggtaaag
      841 gaagatttca aacccatttc taacgaagaa accatgccaa ctacagaagt gttcaaaagc
      901 gttaaaagct tacaaatttt aatagggctg tttgtaacaa gcatgattat tcaaatttct
      961 gcacaatcaa ttgcacctat tttaacactt tacattcgtc atttaggaca gactgaaaac
     1021 ttgatgtttg tttccggatt aattgtttct ggtatgggat tctctagtat tctgagtagt
     1081 ccaaagctag gaagaatcgg ggatcgcata ggcaatcatc gtttgctatt attagctcta
     1141 ctgtatagtt tcctcatgta tgtcttatgt tcattggcac agacttcatt acagctaggg
     1201 gttattagat tcctgtatgg ttttggaaca ggtgccttga tgccgagtat taattcaatc
     1261 ttgactaaaa ttgctccaag acaaggttta tcaaggattt tcagttataa ccaaatgttt
     1321 agcaatttag gacaagtatt aggtcctttt gtcggttcag cagtttctat tcatttagga
     1381 ttccgatggg ttttctttgt gactagcttc atcgttttag ctaattttgt ttggtgtttt
     1441 attaatttta gaaaatatat tagagttaag gaaattgtgt gagagttaaa attaatttaa
     1501 agtgtagctc ttgtggtagt aagaattatt taaccagtaa gaataaaagt aatcatcccg
     1561 ataaaatcga ggttccaaag tattgcccaa aggagagaaa agtaacacta catattgaat
     1621 cttaataagt tttatgttag aatgtacttt gaccatagtg gaggaattaa catgtataac
     1681 ctattactca ccattctact tgttttatca gtattattga ttatctcaat tttcatgcaa
     1741 ccacaaaaga atccaagtag taatgttttt gatagtagcg gttctgaagc cctttttgaa
     1801 cgtagtaaag ctcgaggatt tgaagctttt atgcaacgtt ttacaggtgt tttagtcttt
     1861 ttctggctac taatcggctt agtattatct attttatcaa gtcattaatg atagctaaat
     1921 acagataagt gaggatgggg gatagcccaa cctcactttt tgttagtaga atggttgtag
     1981 agtaataata aagtgactaa agttactaga aaaggaaata atgaaagaaa aaattataaa
     2041 atatttggaa gaacacggta aaaccagcgt taatgattta gctgctgccc tagatatggc
     2101 aggcgcaaaa gcatttccaa aattaatcaa aacaatctca aatttggaga gccacaggca
     2161 attaagattt gacgataatg gaagtctttc tcttcaaaag aaagaagcta agaaaaagga
     2221 aatcacagta aggggtcttt ttagagcaaa taaagcagga tttggttttc ttagtattga
     2281 ccaagacgaa gatgatatgt ttatcggaaa aaacgatatt gcttatgcta ttgatggtga
     2341 cacggtagaa gctgttgtaa agaaaccagc ggaccgtctt aacgggactg cagcagaagt
     2401 gcgtgttgtt aatattgttg aacgtagttt aaagacgctt gttggtaagt ttgtcttaga
     2461 cgatgagcgc cctaaatacg caggatatat caaatcgaaa aatcaaaaaa tcaatcaaaa
     2521 gatttatata cgcaaagaac cggtcgtttt agacggaaca gaaattatca aagtagatat
     2581 tgataaatat ccgacacgag gacatgatta cttcgtagca agtgttcgtg atatcgttgg
     2641 acatcaagga gatgttggta ttgatgtttt agaagtcttg gaaagtatgg atatcgtctc
     2701 agaatttcct gaggacgtta ttgcagaagc caatgctatc ccagatgctc caacggagaa
     2761 agatttaatt gggcgtgttg atttacgtca agaggtcact ttcactattg atggagcgga
     2821 cgccaaagat ttagacgatg ctgtgcatat taaattactg gataatggtc attttgaact
     2881 tggagttcat atcgcagatg tatcatacta cgttactgaa ggctctgcct taaatcgtga
     2941 agcacttagt cgtggtacgt ccgtatatgt cacagatcgc gttgtaccaa tgttaccaga
     3001 gcgtctatct aacggtatat gttcattaaa tccaaatctt gatcgtttaa cgcagtcttg
     3061 tattatggaa attgaccaaa atgggagagt tgttaatcat caaattacac agtcagtgat
     3121 taataccact tatcgtatga cttataccgc tgtaaatgat attattgctg gcgatgagga
     3181 aatctgctca gaatatgagt ctattgtgtc ctcagtacag catatggtca ctttacacca
     3241 tactttggaa gctatgcgta cgcgtcgtgg agcgctgaat tttgacacat ctgaagcaaa
     3301 aattatggtt aatgataagg gaatgccagt tgatattgtt attcgcaaca gaggcattgc
     3361 tgaacgcatg attgagtcat ttatgttggc ggcaaatgaa acggttgctg aacattatgc
     3421 tcgtctaaaa ctgccattta tttatcgtat acacgaagag cctaaagctg aaaaattaca
     3481 aaaattcatc gattatgctt ctgttttcgg tgttcagatt caaggaacag caactaaaat
     3541 cacccaatct gctttacagg actttatgaa aaaagtacaa gggcagccag gaagtgaagt
     3601 gttatctatg atgttattgc gctcgatgca acaagctaga tattctgaac acaaccatgg
     3661 acattatgga cttgctgcgg aatattacac tcactttact agccccattc gacgttatcc
     3721 agacttgtta gttcatcgta tgattcgtga ttatgatgat aaagcgatgg ataaagctga
     3781 tcattttgct aaccttatcc cagaaattgc aactcaaacg tctagtcttg agagacgtgc
     3841 cattgatgct gaacgtattg tagaggcaat gaagaaagcc gaatatatgg aagaatatgt
     3901 tggtgaggaa tttgaaggtg tggttgcaag cgttgttaaa tttggtatgt ttgtagaatt
     3961 accaaatact attgaaggat tgattcacgt aacgaccttg cctgaatatt atcactttaa
     4021 tgaacgtacg ctgacgcttc aaggtgagaa atctgggaaa gttttccgtg ttggtcaaca
     4081 aattaaggtt aagttgattc gttctgataa ggaaactggt gatatcgatt ttgattattt
     4141 accatcagat tttgatattg ttgaaaaggt aagtaaaagt tcgcgtgagg gtcgtcctaa
     4201 tagaagttct aaacgagaac atcagcatag aatttctgat cgtgataata agaataaaaa
     4261 tacatctaag aaaaaagcat ctcgaaaacc caaaaggaac tcggatagca aatcacatca
     4321 tcataaagac gatagaacaa caggttcaac caagaaaaaa acgaaaaaac ctttctataa
     4381 aggcgttgct aagaaaggtc agaaacggaa atctaagaaa ggttaagtat ggttaaaggt
     4441 caaggaaacg ttgtagcaca aaataaaaaa gcgcatcacg actatactat cgttgaaaca
     4501 atagaagcag gaattgtttt gacaggaaca gaaatcaaaa gtgtccgcgc agcgcgtatt
     4561 acattgaaag atggatatgc acaaataaaa aatggtgaag cgtggttgat taatgttcat
     4621 attacaccat atgatcaagg taacatatgg aatcaagatc ctgatcgtac acgaaagtta
     4681 ttactaaaaa aacgtgagat tgaaaaaata tcaaatgaac tgaaagggac tggtatgact
     4741 ctggtacctc taaaagttta cttaaaagat ggttttgcta aagttctatt gggtttggca
     4801 aaaggaaaac atgattatga taagcgagag tctatcaaac gtcgtgagca aaaccgtgat
     4861 attgcccgtc agttaaaaaa ctataattca cgatgattat caaaaggtta ggcaattgcc
     4921 taaccttttt tggacgaaaa aagtgaagtt gattgaccaa cttcactaat aatatagact
     4981 taatgtgatt tatgtttgaa gtgactatat aaaccaaatc caataatcca gatggttgct
     5041 ccaatagccc cgatgtatgt tgaatcttgc aagaagagag aaacgaatac aaagaggaag
     5101 aagagaagtg tgattggtga aaggattttg taggcaggca ttttataacc atcttccata
     5161 aaatcaggtg atttacgata tttccaatgt gcaatcataa taagaacata gatagaaatg
     5221 taaacaccag aagatgatgc agtaattaag gagaaggcat ctgaaatacc aggtaaaaca
     5281 ctgataagtg ctgaagctcc aacgattaca gctgatgcaa taatagctcg acttggaacg
     5341 ctttgacgag ataattggtc taattttaac gcttttgtaa gagcatttgg tgattcatta
     5401 gccaattgga ataaatgacg ccctgttgag tacaaagtag aattaagagc agatgctgca
     5461 gacgttagga caacaaagtt aatcaaagcg gcagcccact taatcccagc tagtttgaat
     5521 actgtaacga aaggtgactc attaactggt aactgttgcc atgggaagat ggccataatt
     5581 gctaataatg aacctgcgta gaataaaata atacgcatcg gaatttcttg gattgctttt
     5641 ggtaaaactt tacgtggatt agctgtttca gaagttgtta ctccgacaaa ttcaatagct
     5701 aaataagcaa agaataccat ttggaaagcc atgaagaaat tcaatttacc tttagggaac
     5761 cattcaaaat gattggtgat atttgagatt gaagcatggt atccgtgacc ggtgtcaaag
     5821 tttgtcaata ccataaagat accggttgct attaaaccaa gaattgcaat aacttttatc
     5881 atagcaaacc aaaactcggt ttctccgaat gcttttacag caattaagtt gatagaactt
     5941 aggatagcta aaaaaacaat ttggataatc caggatggcc attcagggaa ccaaaattga
     6001 acatactctc ctacggctgt aatctctgcc ataccgataa atattaggga aatccaataa
     6061 gaaagtgttg agaaaaatcc ccaaccgggt cctaaatatt tggtaatgaa attaataaaa
     6121 gtatgctgat ttggatcttg ataaagcatc tctccaatag cacgcatcat gaagaacatg
     6181 aaagcgccag tgatagcata gacaagaact attgaaggac ccgtcaggct aattgaacga
     6241 cctgcaccaa gaaaaagccc agttccgatt gtccctgcaa tcgcaattaa ctgtacgtga
     6301 cgattttcta ggccgcgtac cataccattc tcagattttt gagtgtggtc attattattt
     6361 tttgacatta taaatactcc atctattttt gaaaaactcc tttattatat aatttttttg
     6421 caaaattcgc aataattact atacaatatt tttctatgat aaaatctcct agctaagtga
     6481 gctaagagat ttcaattcat ccctcttcct tgacaatata gactgggcgt tttttagttt
     6541 ctaggaaaat cttagagatg tatttgccta taattcctag cgacaataac tgaataccac
     6601 ccataaaaag tacaatggta actgtgcttg cccaacctga aacgggatcc ccaaatagta
     6661 attttcgaat tataataaaa atgatagcta aaagtgatag tactgaacta agggttccaa
     6721 tccaggtagc tatagctaat ggaatttctg agaagttgat gaaaccgtct agagagtatt
     6781 ttaagagatt aaagaaactc catgaggtct tacccgcaat tcgttctcga ttctcataac
     6841 taatataagc gacatcatat ccaatccagg aaaaaatccc ttttgagaag cgattcactt
     6901 catttagctc gagaatactg tcaacaactt gttttgtcat gagacgaaaa tcacgcgcac
     6961 catcaaccat tttagtatct gacacttgat tgattaattt ataaaaaagc ttggcaaaaa
     7021 gagagcgtat caacggctcc cctttacgat cttttcgcct tgtagcgaca atatcataac
     7081 cttcttgaag ttttaggtac atttctggta ataaagtggg tggatcttgc aagtcaacat
     7141 ccataacagt gataaaatca ccagtagtat gttctaaacc agccaataaa gcagcatctt
     7201 taccaaaatg gcgtgaaaat gaaaggtaat gaacattgtc aaattgttta gctgctttct
     7261 taagaagttc gagtgtacga tcagtagaac catcgtttac gaaaatgtat tcaaaatggg
     7321 tgtaacgagc catttcggat tcaacttttt tgatttctgt taaaaaagtt gagacagtct
     7381 cttgttcatt ataacaaggg acaataactg ataataaagc catttgtaat tctccgtatc
     7441 attcttgctt aaattttaac ataatatggg aaaaaataga aagattataa ggtaatcata
     7501 aaaaggacta ggtttttgcc tcagtcctta acttaatatg atgagatgtt attagtcagc
     7561 tttaaattga ctgagtcgaa taacttcaat tttatagcca tctggatctt gaataaagta
     7621 gaacatatta ggtttacctg gtaatccaga aatttttgtt acagagtaac ctgctttctg
     7681 atgggcatca taagtagttt ccaaatcgtc tacaccaaca gcaatatgac catacccatt
     7741 tcctaaatca taagcttcgt gatcatagtt ataggttaac tctaattcgt aactagggtc
     7801 gtcctctagg gccatataga ctaaagtaaa ttgattttca ggaaagtcat tacgacgaac
     7861 ttccttaaaa ccaagtgctt cttgataaaa agcaattgag gcgtccaaat ctttaacgcg
     7921 aatacaagta tgtaaaaatg gcatagtctc tctcctattt ctttattaga cgtattatgt
     7981 caaaataaaa ttgtcttttc aaaaatattg attataaatt aggctgtcta tttactcaac
     8041 ccaagtaatg acttcttggc gttctttcct agcacgggaa tgtttaggtt cacgcaaacg
     8101 ataaccaaat gaaaccatac aagaaatagc ttctttctta tcgtctatta atccttcttt
     8161 actaaggata ttattgacct tttcatagtc aaatccttca attgggcatg aatctacgcc
     8221 gatcatagca gcagccgtca tcatattacc gagtgcgata taagtttgtt ttgctgtcca
     8281 atcccaaaga gaacgttcag aatcaatttt catatcgttt tcttggaagg atttgtagag
     8341 aggtatccta ctctcaaggg cagctggatc acctagacca cgtcgaatga gactgttaat
     8401 catgctatca gcgttataat aagcaccttt ttcagctagt agtaaaacaa agtgactagc
     8461 agtatctagt tgatattgtg ctccccaagc aacttcctta agtttatctc gaaattttgc
     8521 aattgtttgt ctatctagta ccaaaaatcg ccagccttct aaaccgactg aagaaggact
     8581 caaccaagca gtatctaaaa tatactccat atcttcttta ggaatatcat tattattata
     8641 aacacgaatc gccatacgaa aatcaaaagc tcttcgaact tgttttttga tatcttcttt
     8701 tgtcatttta gatcctcctg ttattaatgc tatcatatca caatttagaa aaaatctcag
     8761 caggcgatat cgtagcaaag tagagctaca aaattctaat gactgtcaga aaaataaagg
     8821 gaatgaacaa aatgataggg ttttgatgaa gaaagttata taattttaca taaggagggg
     8881 ataataccat atatcaaact atcatttaga tgtttaaaat agatgttttt cactatgaaa
     8941 ttcatttttg tcacatagaa aaggagattg ttatggaaac atatacttta tcgaatactc
     9001 ttaacattcc taaaattggt tttgggacat ggcaactaac tgaaggtgaa gaagcttata
     9061 aggcagtgac tcatgcttta aaagttggtt atactcatat tgatactgct cagatttatg
     9121 gtaatgaaca tagtgttgga cgtgctataa gagacagtgg tttggcaaga gaaagtatct
     9181 ttttgacaac taagatttgg aatgacaaac atgactatca tttagccaaa gcttctattg
     9241 atgaatctct gcagaaatta ggtgttgatt atattgatct tcttttgatt cattggccaa
     9301 atccaaaagc tcttcgtgaa aatgatgcgt ggaaagctgg gaatgcaggc acttggaaag
     9361 cgatggaaga agcttataaa gaaggaaagg taaaagctat tggtgtttcg aatttcatga
     9421 aacatcactt agaggcttta tttgaaactg ccgaaataaa acctatggtt aatcaaatta
     9481 tcctagcacc aggttgtgct caagaggatt tggttcgctt ctgtaaagga aatgatatct
     9541 tattagaagc ttacagtcca tttggaacag gtgctatttt tgaaaatgaa tctataaaag
     9601 ctattgctga aaaatatggt aaatcagttg cgcaagtagc tcttcgctgg tcacttgata
     9661 atggcttttt accattacct aagtcagcga ctcccaaaaa tattgaagca aaccttgata
     9721 tttttgattt tcaattaaac gaagatgata ttgctacctt aatacaacta gattcaggta
     9781 ttaaacccaa agatccagat aatgtctcat tttaataaga aaagatggaa atgtatttcc
     9841 atctttttta tgtgggatag ttccttaaag ttaagaaaac ca
//
LOCUS       DATRMA010000073         9800 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.073, whole genome shotgun sequence.
ACCESSION   DATRMA010000073 DATRMA010000000
VERSION     DATRMA010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9800
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.073"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            complement(79..1404)
                     /gene="gtfB"
                     /locus_tag="VBC28_001661"
     CDS             complement(79..1404)
                     /gene="gtfB"
                     /locus_tag="VBC28_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system glycosylation chaperone
                     GtfB"
                     /protein_id="HEO6815627.1"
                     /translation="MINLFDSYTQSSWDLHFSLIKSGYINPTIALNDDGFLPDDVTSP
                     YLYYTGFAKTGAGRPLYYNELRVPDTWEIIGFSSGADIVDLGVKKGRIIYANPNHKRL
                     IKEVDWFDEQGRVILKDRFNKFGFCFAQTFYNADGQAIQTSYYNKDRQEVISENHMTG
                     DYILNDNNQFKVFKSKVEFVINYLQEAKFNLDRIFYNSLSTPFLVSFYLNRLESKDVL
                     FWQEPLVDDIPGNMRLLLNNPSPNTKIVIQSYEAYANAMRLLTDEEQKQVSFLGFMYP
                     LKETEKLHNQALILTNSDQIEALESLVTSLPNLTFNIGALTEMSSDLMNFGKYDNVVL
                     YPNITTNQIQYLSNICAFYLDINHHNEILSAVRSAFEHQQLIFAFEETSHQIRFVSPK
                     NIFPKKDIFTFISHLQPLIGNKCNIEKALKQQLEDCHVSSSTQYQSVIN"
     gene            complement(1397..2905)
                     /gene="gtfA"
                     /locus_tag="VBC28_001662"
     CDS             complement(1397..2905)
                     /gene="gtfA"
                     /locus_tag="VBC28_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system glycosyltransferase GtfA"
                     /protein_id="HEO6815628.1"
                     /translation="MTVYNINHGIGWASSGVEYAQAYRGSVLRKLGIDAKFIFTDFFS
                     ADNIISLTRNIGFQDKEIIWLYNFFSDIELAPTTFSIEDLQKQYLGKLVRKEDKGRVI
                     KFFYEDENIYLTVYLDNFNKDKVHRVEIVSNNNLIRKDYYSYTRTFSEYYYPKDGVAH
                     LYQRRFYNEDESTAFLEMVENSSSRFIINGRLLPSKVAFFDYFLESMTFTSKDIILLD
                     RGTDTAQSLLRHGKPAKLGTVVHAEHFSENAVTADTILWNNYYDYQFTNANRFDFFIT
                     STDKQTELLEQQFKQFTNHNPRIITIPVGSIDNLKMPMDNRRPYSILTASRLASEKHV
                     DWLVRAVIRIREILPEVTFDIYGSGGEEEKIRNIINAANATEYIRLMGHKNLSNVYQN
                     YELYLTASKSEGFGLTLLEAIGAGLPLIGFDVRYGNQTFIKDGENGYLIPRFDMDDEE
                     AIVEAFKEKVLQLFQQDQKALREASYAIAEGFLTNEVEGKWYNLVKELVQDD"
     gene            complement(2919..5306)
                     /gene="secA2"
                     /locus_tag="VBC28_001663"
     CDS             complement(2919..5306)
                     /gene="secA2"
                     /locus_tag="VBC28_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system translocase SecA2"
                     /protein_id="HEO6815629.1"
                     /translation="MIAFNSLFSLDKKRLKKLQRTLNTINSLKGQMATLSNEELQAKT
                     TEFRKRLVNGETLDDICAEAFAVVREADERVLGLFPYDVQVIGGLVLHQGNTAEMKTG
                     EGKTLTATMPLYLNALEGKGAMLLTNNSYLAIRDAEEMGKVYRFLGLSVGVGVSDNEE
                     EDRDAATKRAVYSSDIVYSTSSALGFDYLIDNLASSKSQKYMPKLHYAIVDEADAVLL
                     DMAQTPLVISGSPRVQSNLYKIADELILSFEEQVDYYFDKERQEVWIKNQGVREAERY
                     FRIPHFYKQSNRELVRHLNLSLKAHKLFERGKDYVVDDGEIKLLDATNGRVLEGTKLQ
                     GGVHQAIEQKEHLNVTPESRAMASITYQNLFRMFTKLAGMTGTGKTAEKEFIEVYDME
                     VVRIPTNSPVRRIDYPDKIYTTLPEKIHATIEFVKQVHDTGQPILLVAGSVRMSELFS
                     ELLLLSGIPHSLLNAQSAVKEAQMIAEAGQKGAVTVATNMAGRGTDIKLGKGVSELGG
                     LAVIGTERMKSQRMDLQLRGRSGRQGDIGFSQFFVSFEDDLMIESGPKWAQDYFRKNR
                     DKVNPEKPKALGQRRFQKLFQQTQEASDGKGESARSQTIEFDSSVQLQREYVYRERNA
                     LINGESGHFSPRQIIDTVISSFIAYLDGEVEKEELIFEVNRFIFDNMSYNLQGLSKEM
                     SLEEIKNYLFKIADEILREKHNLLGDSFGDFERTAALKAIDEAWIEEVDYLQQLRTVA
                     TARQTAQRNPVFEYHKEAYKSYNIMKKEIREQTFRNLLLSEVSFNENGDLQIYFI"
     gene            complement(5293..6285)
                     /gene="asp3"
                     /locus_tag="VBC28_001664"
     CDS             complement(5293..6285)
                     /gene="asp3"
                     /locus_tag="VBC28_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp3"
                     /protein_id="HEO6815630.1"
                     /translation="MSTISYIYWDDFSRFSYNFGTKLQFLGKSVCFENPLAPSSTNLY
                     TWSSQTNYQSKRISPNLPLLRKGTRYSLSLNAELDLVSSLFVRIEFYNRFNESVGFEL
                     LKKDSIIFIYPKEAYTYTISLINAGCSDFTFHYLKLEEVTDSVIQEKNLSTEFTIEEH
                     QDVLNLLLVEKKDSVYINKIESISQLQQKVELVSNPSLNSDSLILPELEKGLEDALKV
                     FPNIKINVIAYGTQGNFAALYYAKKFPRITAYINDCFAPFGILLKSLPHLTAKQQIFL
                     REVWDTRETSPNVKHYGLVSENSSLNLVSMILSGNEHLPYLTLLKKQDDNYDSL"
     gene            complement(6282..7841)
                     /gene="asp2"
                     /locus_tag="VBC28_001665"
     CDS             complement(6282..7841)
                     /gene="asp2"
                     /locus_tag="VBC28_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp2"
                     /protein_id="HEO6815631.1"
                     /translation="MSKIKLTILQVGEENWATKENIPNNMEWLFIKPDQISDFVTTEN
                     NYLTSSKLLQKLPRKISALLLTEQTYGPELSSLSSFFEVYEVFYPKDKHATGITEEFL
                     RSKMAQRYDSSSPDQLIRQFYKGLFIGQYGEKLQVSQIQIRNDFEGVVNYQGNNYLEL
                     EGQFGENYSFLLNFAYNIPFSSDFYNELFLEHIIEGDIDIRLVISLIVDGSVDDIAKE
                     WYFEKEDLNQLISLESDISGSLAVKLFAKGKGIVKLGPLHRRNGRGGLGTFLLGGERH
                     IDAIGHEFMTYFDPVDFKPPLTVYFSGFRSAEGFEGFWMMKSMKTPFMLICDPRLQGG
                     AFYIGSKEYEQKIVDAIQEKLAFLNFSSDQLILSGLSMGTYGATYHGAKLNPHAIIIG
                     KPIFNLGTVAQRERLERPDGFATSLDIQLLNQGDLTSSSSEKLNNYFWKSIEEGDFSN
                     TTFALAYMKNDDYDATAFSDLLQYFRGKKHKILGRGWDGRHGDCSAEVGAWFTSQYRR
                     MLSNDFGRKEE"
     gene            complement(7848..9392)
                     /gene="asp1"
                     /locus_tag="VBC28_001666"
     CDS             complement(7848..9392)
                     /gene="asp1"
                     /locus_tag="VBC28_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp1"
                     /protein_id="HEO6815632.1"
                     /translation="MFHFIPSWYNENRTWYDNNYLWYFKPTNVGFDDTINQMKMFDYA
                     GKESRLVVLNYMPNLRYYLHRYDLLESGYYSVFDDIQEIGNVRQQMIDFRQLNWPEGV
                     DFTYTPFIVLVKKSGDLIAKVQFGEEGNLTHIDYFANEQIAKKYLFDDRGFLSSILYY
                     DNGGEAYQDYLAPSGERIMREYLREGDHHVEINPKKAIHFLKLSYSDIEELIREKYLT
                     YLHKEVSKSDTIIVSFNQVHNAFIVGNTSKGNLILSVFSERNNAHNVLEDYSSLSRAD
                     AIICDRLDIAAQLKEKIDKPVVHVSPFDTRLALGKSNQVRDLEIYFVVDRLSHKELQK
                     SLTSLYKVMLKNNDIKVTFVSYEREFESRQLTYDYLKEATKVFDQKFFSLSDDDANQV
                     LMQSDEVEKEKTRLSFTHPLSETDIINRLEYVRLIIDISKIPDLYTQIAGISSGIPQI
                     NTILTEFVEHRKNGYVIEEIQELEKAIPYYCEQLTNWNRSLIYSIDKINDYTGGQLVE
                     RIINSY"
     gene            complement(9392..>9800)
                     /locus_tag="VBC28_001667"
     CDS             complement(9392..>9800)
                     /locus_tag="VBC28_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="accessory Sec system protein translocase subunit
                     SecY2"
                     /protein_id="HEO6815633.1"
                     /translation="YNDYFSLSSIQGVVIYMILMLVLSVAFTFVNIDPTKISEAMRES
                     GDFIPNYRPGKETQSYLSKICYLFGTFSGFFMAFLGGVPLLFALGNDDLRTVSSMTGI
                     FMMITGMSFMILDEFQVIRIRKQYTSVFENEEN"
BASE COUNT         3226 a         1877 c         1423 g         3274 t
ORIGIN      
        1 ggaatttgat atcgctcttc agatagacgt ttattaatac tctcctgaag taaattcttt
       61 tttttcaaaa ttttcccctt aattaattac agattgatac tgagtagagc ttgaaacatg
      121 acaatcttca agctgctgtt ttaatgcttt ttctatatta catttattgc caattagtgg
      181 ctgcaagtgc gaaataaatg taaaaatatc tttttttgga aaaatatttt taggtgatac
      241 aaaacgaatt tgatgactag tctcttcgaa agcaaaaatt aactgttgat gttcaaaagc
      301 agagcgaact gctgataata tctcattatg atgattaata tcaagataaa aagcacaaat
      361 atttgataaa tattgtattt gatttgttgt gatatttggg tagagtacaa cattatcata
      421 tttgccaaaa ttcatgagat cactagacat ctcggtcaaa gcacctatgt taaaagtcaa
      481 attaggaaga gaagtcacta aactttctaa tgcttcgatt tgatcagaat tcgtcaaaat
      541 aagagcttgg ttatgtaatt tttctgtctc tttaagagga tacataaatc ctaaaaagct
      601 aacttgtttt tgttcttcgt cagttaataa cctcatagca ttagcataag cttcataact
      661 ttgaattaca attttagtgt ttggggaagg attgttcaat aataagcgca tatttcctgg
      721 aatatcatct acgagaggct cctgccaaaa gagaacatct ttactctcta gacgattaag
      781 gtaaaagctt actaaaaatg gtgttgacag agaattatag aagattctat ctaagttaaa
      841 ttttgcttct tgaagataat ttatgacaaa ttcaacttta gatttaaaaa ccttaaactg
      901 attattatca tttaaaatat aatcccctgt catatggttc tctgatataa cctcttgcct
      961 atccttatta taatatgacg tctgaatcgc ttgaccatcc gcgttataaa aggtttgtgc
     1021 aaaacagaac ccaaacttat taaagcgatc ttttaaaatg actctacctt gctcatcaaa
     1081 ccagtctacc tcttttatta gacgcttatg atttgggttt gcataaataa ttctgccctt
     1141 tttgacacct aagtcaacaa tatcagcacc actactaaaa ccaataattt cccatgtatc
     1201 aggaactcta agttcattgt agtaaagtgg gcggccagca cctgtctttg caaaaccagt
     1261 gtaatataaa tagggtgatg ttacgtcgtc tggtaaaaaa ccatcatcat tcaaagctat
     1321 agtagggtta atataacctg attttatcaa ggaaaaatgt aaatcccaac ttgattgagt
     1381 gtaactatca aataagttaa tcatcttgta ccaactcctt aactaagtta taccattttc
     1441 cttctacttc atttgttaag aatccttcgg caatggcgta agaagcttct cgtaaagcct
     1501 tttgatcctg ttggaataat tgtaacactt tctctttaaa agcttctaca atagcttcct
     1561 catcatccat atcaaatcga ggaattagat aaccattttc tccgtctttg ataaaagttt
     1621 gattaccata acgaacatca aacccaatca aaggaagtcc tgcgccaata gcttcaagta
     1681 aagttaagcc aaacccctca cttttagaag ctgtcaaata taactcataa ttttgataaa
     1741 cattcgagag atttttatgt cccatcaatc gaatgtattc cgttgcattg gctgcattta
     1801 taatatttct aattttttct tcttctccac ctgatccata gatatcaaag gtcacttcag
     1861 gaagaatttc tcttatccta ataactgcac gtactaacca atctacatgt ttttcactag
     1921 ctaggcgtga agctgtcaaa atagagtacg gacggcgatt gtccattggc attttaagat
     1981 tgtcaattga gcctaccggg atagttataa ttctaggatt atgatttgta aattgtttaa
     2041 attgttgttc caaaagttct gtttgtttat cagtggaggt aataaaaaaa tcaaatctat
     2101 tagcgttagt aaactgataa tcgtaatagt tattccataa aatagtgtca gcagtcacag
     2161 cattctcact aaaatgttcc gcatgaacaa cagtccctag cttagcgggt ttaccatggc
     2221 gcaaaagact ctgagcggta tcagttcctc tatccaaaag aataatatct tttgaagtga
     2281 aagtcattga ttccaaaaaa taatcaaaaa aagctacttt ggatggtaaa agtctgccat
     2341 tgataataaa acgacttgag ctattttcta ccatctccaa aaaggctgta ctttcatctt
     2401 cattgtagaa tctacgttga taaaggtgag ctacaccatc tttaggatag taatactctg
     2461 agaaagtacg tgtataacta taataatctt ttctgataag gttgttattt gaaacgattt
     2521 ctacacgatg cactttatcc ttgttaaaat tatcaaggta aactgttaaa tagatatttt
     2581 catcttcgta gaaaaacttg attacccgac ctttatcttc ttttcgaaca agttttccaa
     2641 gatattgttt ttgcaaatct tctatactaa aagttgtcgg ggcaagctca atatctgaaa
     2701 aaaaattata taaccaaata atttctttat cctgaaaacc tatatttcgc gtgaggctaa
     2761 taatattatc agctgaaaaa aaatctgtaa aaataaactt agcatcaatt ccaagttttc
     2821 tcaaaacaga acctcggtat gcttgagcgt actcaacacc gctgcttgcc caaccaattc
     2881 catggtttat attataaact gtcattatct ttcatctctt atataaaata aatttgaaga
     2941 tctccattct cattgaagga tacttcactc aaaagtagat ttctaaaagt ttgttcacga
     3001 atttcttttt tcatgatatt gtaggattta tatgcttctt tatgatattc gaaaaccggg
     3061 ttacgctgtg ccgtttgacg agctgttgcc acagtacgta attgttgtag ataatctact
     3121 tcttcaatcc aagcttcatc aatcgctttt aaagcagcag tacgttcaaa gtcaccaaaa
     3181 ctatcaccaa gcaaattatg cttttctcga agaatctcat cggctatttt gaataaatag
     3241 tttttaattt cttcaagtga catctcttta gaaagccctt gaagattgta agacatatta
     3301 tcaaatataa aacgatttac ttcaaaaatg agttcttctt tttctacttc accatctaaa
     3361 taagcaatga aggaagaaat cactgtatca ataatctgtc ttggactaaa atgtccactt
     3421 tctccattga tcaaagcatt tctctcacga taaacatact ctcgctgaag ctgtacactc
     3481 gaatcaaact ctatcgtttg actacgtgca gactctccct taccgtcact agcttcctga
     3541 gtttgctgga ataacttctg aaatcttctc tgacctaagg ctttaggttt ttctggatta
     3601 actttgtcac gatttttcct aaagtaatct tgcgcccatt taggacctga ttctatcatt
     3661 agatcatctt caaatgatac aaaaaattga ctaaaaccaa tatcgccttg tcgacccgaa
     3721 cgtcccctta attgtaaatc cattctttga ctcttcattc tctccgttcc aataactgca
     3781 agaccaccta attctgatac tcccttacct aacttaatat ctgtaccacg accagccata
     3841 ttcgttgcaa cagtgaccgc acctttttgc cctgcttctg caatcatctg tgcttcctta
     3901 accgcacttt gagcatttaa caggctatga gggataccac ttagtaataa taattctgag
     3961 aatagctctg acattctcac agaaccggca actagtagga ttggttgtcc tgtatcatga
     4021 acctgtttaa caaattcaat cgttgcatgg attttctctg gtaaggttgt ataaatctta
     4081 tcaggatagt caattcgtct aacaggagaa ttagttggaa ttctaactac ttccatatca
     4141 tagacctcaa taaactcttt ttcagcagtt tttccagttc ctgtcattcc agccaattta
     4201 gtaaacattc taaaaagatt ttgataggta atggaagcca ttgctcgaga ctctggtgtg
     4261 acattaagat gctctttttg ctcaatagct tgatgaacac ctccttgaag tttagtacct
     4321 tctaaaacac ggccattagt cgcatctagc aacttgattt caccatcgtc aaccacataa
     4381 tcttttcctc gttcaaataa tttatgtgct tttaaagata agttcaaatg tcgcacaagt
     4441 tctcgattag actgtttata aaaatgaggg attctaaaat atctctcagc ttcacgaaca
     4501 ccctggtttt ttatccacac ttcctgacgc tctttgtcaa aataataatc aacttgttct
     4561 tcaaaactaa gaattagttc atctgctatt ttatagagat tagattgtac tcgaggtgaa
     4621 cctgaaataa ctaaaggagt ctgggccata tctagaagaa ctgcatcagc ttcatctaca
     4681 atagcataat gtaacttggg catatatttt tgagatttag atgaagctag gttatcaatt
     4741 aaataatcaa agcctagtgc actacttgta ctatagacaa tatccgaact ataaacagct
     4801 cgcttagtcg cagcatctct atcctcttcc tcattatcag aaactcccac accgacactc
     4861 aatcctaaaa aacgatatac ttttcccatt tcttccgcat ctcgaatagc aaggtaggaa
     4921 ttattagtca gcaacattgc ccccttccct tctaaagcat tgagatacaa aggcatagtg
     4981 gcagttaatg tttttccttc accagttttc atctcagcag tatttccttg gtgcagaaca
     5041 agaccaccga ttacctgtac atcatacgga aatagaccta atacgcgctc atcagcttcc
     5101 ctaacaacag caaaagcttc agcacaaata tcatctagtg tttccccatt aactaaacgt
     5161 tttctaaatt cagttgtctt agcttgcaac tcctcatttg atagtgttgc catttggccc
     5221 tttaaactat tgatagtatt taaagtacgc tgaagttttt ttaaacgttt cttatctaac
     5281 gaaaataaac tattaaaggc tatcataatt gtcgtcctgc ttttttaaaa gtgtgaggta
     5341 aggtagatgt tcattaccag ataagatcat tgatactaaa tttaaagagg aattttctga
     5401 aaccaatcca tagtgcttta catttggaga cgtctctctc gtatcccaga cttctctaag
     5461 aaatatttgt tgtttggcag tcaagtgtgg taaggattta agtaaaattc cgaaaggagc
     5521 aaaacaatca ttaatatagg cagttattct gggaaatttt ttagcataat ataaagcagc
     5581 aaaattacct tgtgttccat acgcaatgac attaatctta atgttaggaa aaacttttaa
     5641 tgcatcttct aacccctttt ccaactcagg taatatcaaa ctatcagaat ttaatgaggg
     5701 attgctgacc aactcgactt tctgttgcaa ttgagaaatg ctttctattt tattaatata
     5761 tacactatct tttttctcga ccaaaagaag atttaaaacg tcttgatgtt cttcaatagt
     5821 aaactctgtc gatagatttt tctcctgaat aacgctatct gtaacttcct caagtttcaa
     5881 ataatgaaaa gtaaaatcgc tacaacctgc attaatcaaa ctaatggtat aggtataagc
     5941 ctctttagga tagataaaaa taatactatc tttttttaac agttcaaaac caacagactc
     6001 attaaagcga ttgtaaaatt caatgcgaac aaaaagagag ctaactaaat cgagttctgc
     6061 atttaatgac aagctataac gagttccctt tcttagtaaa ggtaagttag gagaaattct
     6121 ttttgactga taattagtct gggagctcca tgtatacaag tttgtgctag aaggagctaa
     6181 tggattttca aagcatactg attttcctaa aaattgtaat tttgttccaa aattataaga
     6241 aaaccttgaa aaatcatccc aataaatgta gcttatcgta ctcattcttc tttccttcca
     6301 aaatcattag atagcattcg cctatactga cttgtaaacc atgcaccaac ttctgcagaa
     6361 caatcaccat gtcggccatc ccaacctctg cctaatatct tatgcttctt tcctctaaaa
     6421 tattgcaata aatcagaaaa ggcagtcgca tcatagtcat cgtttttcat ataggctaag
     6481 gcaaaagtcg tatttgaaaa atctccttct tctatagatt tccaaaaata gttatttaac
     6541 ttctcagaag aggaactggt caagtcacct tgattgagta gttgaatatc taatgaagta
     6601 gcaaatccat ctggtctctc taatctttca cgttgagcaa ctgttcctaa attaaatatt
     6661 ggtttaccaa taataatagc atgaggattt agcttagcac catggtaagt agcaccataa
     6721 gtccccattg aaagtccgga taaaattaac tgatcagaac taaaatttaa aaaggccagt
     6781 ttttcttgaa tagcatcaac tattttttgt tcatattctt tgcttcctat ataaaaggca
     6841 ccaccttgta atcgtggatc acaaattaac atgaatggag tcttcatact cttcatcatc
     6901 cagaatccct caaagccttc agctgaacga aatcccgaaa aataaacagt tagaggtggt
     6961 ttgaaatcta ctggatcaaa ataagtcata aattcatgac ctatagcatc tatgtgacgt
     7021 tctcctccta gtagaaatgt gcccaatcct cctctaccat tacgacggtg caatggacct
     7081 aacttaacga taccctttcc cttagcaaaa agtttaactg caaggctacc actaatatcg
     7141 gactctaaag aaataagttg atttaggtct tccttctcaa agtaccactc tttagcaata
     7201 tcatcaacag aaccatcaac aatcaacgag ataaccaatc gaatatctat atcaccctct
     7261 ataatgtgtt ctaaaaataa ctcattataa aaatccgatg aaaatggaat attgtaagca
     7321 aaattaagca aaaaactata gttctcacca aattgccctt ctaattcaag atagttattt
     7381 ccctgatagt ttactacacc ttcaaaatca tttctaattt gtatttgtga aacttgaagt
     7441 ttctctccgt attgtcctat aaaaagccct ttataaaatt gacgaattag ctgatctgga
     7501 gagctactat catatcgttg ggccattttc gagcgtaaaa actcttctgt tatccccgta
     7561 gcatgtttat ctttaggata aaatacttca taaacctcaa agaatgacga taaactagat
     7621 aactctggtc cataagtttg ctctgtcaac aaaagagcag atatttttcg aggtagtttt
     7681 tgcaataact ttgacgatgt aagataatta ttttctgtag ttacaaagtc agaaatttga
     7741 tctggtttaa taaagagcca ttccatatta ttagggatat tctctttagt tgcccagttc
     7801 tcttcaccaa cttgaagaat tgtaagtttt atttttgaca tatttattta ataactattt
     7861 ataattcttt ctaccaattg accacctgta tagtcattga ttttatcaat agaataaatt
     7921 aaagaacggt tccaattagt taattgttca caataataag gaattgcctt ttccaattct
     7981 tggatctctt caataacgta accgtttttc ctgtgttcaa caaattctgt taatattgta
     8041 tttatctgag ggatacctga acttatccct gcaatttgcg tgtataagtc tggtattttc
     8101 gagatatcaa taatcaaacg gacatactct aaccggttta tgatatctgt ttctgacaaa
     8161 ggatgggtaa aagaaaggcg tgttttttcc ttttctactt catcagattg cataagaact
     8221 tgattggcat catcgtctga taaactaaag aatttctgat caaaaacttt tgttgcttct
     8281 ttaaggtagt catatgtcaa ttgccgactt tcaaattctc gttcgtagct gacaaatgtt
     8341 acttttatat cattattttt gagcataacc ttatataagg atgttaaaga cttttgcaat
     8401 tctttatggg aaagtctatc tacgacaaaa tatatttcta aatctctaac ttgattactt
     8461 tttcccaaag ctaaacgcgt atcaaaggga gaaacatgaa caactggttt atctattttt
     8521 tctttcaatt gtgcagcaat atctagtcta tcacaaataa tagcgtctgc tcgagataag
     8581 gaagagtaat cctccaatac attgtgagca ttattcctct ccgagaaaac agataatatt
     8641 aaatttcctt tactagtgtt cccaacaata aatgcgttat gaacttgatt aaagctaaca
     8701 atgatagtat ctgatttact aacttcttta tggagatagg tcagatattt ctctctaatc
     8761 aactcttcaa tatcggagta agataacttt aaaaaatgaa tagctttctt aggattaatt
     8821 tcaacatggt ggtcaccttc tcttaggtat tctctcatga tcctttcccc tgatggtgct
     8881 aaataatctt gataggcttc accaccatta tcataataga gaatactaga tagaaatcct
     8941 ctatcatcaa agaggtattt cttagctatt tgttcatttg caaaataatc aatatgcgtt
     9001 aaatttcctt cttcaccaaa ttgaacctta gcaattaaat ctcctgactt ttttaccaaa
     9061 actataaacg gagtataggt aaagtccacc ccttcaggcc aattgagttg tcggaagtca
     9121 atcatttgct gtcttacatt cccaatttct tgaatatcat caaaaactga ataataacct
     9181 gattctaaca agtcatagcg atgtaaataa taacgtaaat tgggcatata gtttaagaca
     9241 actaatctac tctcttttcc agcataatca aacatcttca tttggttgat agtatcatca
     9301 aaaccgacat tagtcggctt gaaataccac aagtaattat tatcatacca agtacgattt
     9361 tcattatacc aagatggaat aaaatgaaac attaattctc ctcattttca aatacagaag
     9421 tgtattgttt acgaatacga ataacttgga attcatccaa tatcataaat gacatccctg
     9481 tgatcatcat aaaaatccct gtcatagatg aaactgtcct aagatcatca tttccaagag
     9541 caaataataa tggtacccct cctaagaaag ccatgaaaaa accactaaaa gttccaaata
     9601 aataacagat tttcgataaa taactttgtg tttctttacc aggtctataa ttaggaataa
     9661 aatcaccact ttctcgcatt gcctcagaaa ttttagtcgg atcgatattc acaaaagtaa
     9721 atgcaactga taaaacaagc attaaaatca tataaataac cactccctga atagaagata
     9781 aactaaaata gtcattgtat
//
LOCUS       DATRMA010000074         8805 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.074, whole genome shotgun sequence.
ACCESSION   DATRMA010000074 DATRMA010000000
VERSION     DATRMA010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8805
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.074"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            complement(<1..583)
                     /gene="rarD"
                     /locus_tag="VBC28_001668"
     CDS             complement(<1..583)
                     /gene="rarD"
                     /locus_tag="VBC28_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HEO6815634.1"
                     /translation="MRKDNLGIILGLSAYVLWGLLSLYWKLLSGIEAYSTFAYRIIFT
                     VLTMLIYMLVSGRKTVYLKDLKGLVNNKKSFWTMFVASILISINWLVYIFAVTHGHAT
                     EASLGYYMMPIISILLSVLVLREHLARVVSLAILIAIMGVGILVYQTGHFPLISLTLA
                     LSFGFYGLLKKSISLSSDFSMLVESSFIAPFALI"
     gene            complement(570..1436)
                     /locus_tag="VBC28_001669"
     CDS             complement(570..1436)
                     /locus_tag="VBC28_001669"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEO6815635.1"
                     /translation="MRIIVPATSANIGPGFDSIGVALSKYLSIEVLEESTEWLVEHNL
                     VNIPKDHTNLLIQTALHVKSDLAPHRLKMFSDIPLARGLGSSSSVIVAGIELANQLGN
                     LALSQKEKLEIATRLEGHPDNVAPAIFGDLVISSIVKNDIKSLEVMFPDSSFIAFIPN
                     YELKTSDSRNVLPQKLSYEDAVASSSVANVMVASLLKGDLVTAGWAIERDLFHERYRQ
                     PLVKEFGVIKQISTQNGAYATYLSGAGPTVMVLCSKEKEQPIVTELSKLCLDGQIQVL
                     NIERKGVRVEKR"
     gene            complement(1438..2721)
                     /locus_tag="VBC28_001670"
     CDS             complement(1438..2721)
                     /locus_tag="VBC28_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEO6815636.1"
                     /translation="MTIKIALLGFGTVAKGIPYLLKENQHKLLSLEGEDIVIDKVLVR
                     DNESRQRFINQGFTYNFVTEINTILQDPQIDIVVELMGGIEPAKTYLSQALGFGKHIV
                     TANKDLIALHGKELMDLADARGLALFYEGAVAGGIPILRTLSHSFASDKMTRLLGILN
                     GTSNFMLTKMFEEGWSYEQALKKAQELGYAESDPTNDVEGIDTAYKATILSQFGFGMP
                     IDFDDVNYKGISSIRSEDVEVAQEMGFAIKLVADLRETPTGISVDVSPTLISQEHPLA
                     AVNHVMNAVFIESIGIGQSLFYGPGAGQNPTATSVLADIIDISRSIRSQIKIKPMNTY
                     HCPCRLSMQSDIFNEYYLAISLRNAEDSDTLGRYFEQENIGLKNVIEKALDDKQQEIY
                     VLTDEVSQEKITQFIEEFPESGVIQLINVFKVIGG"
     gene            2898..3785
                     /locus_tag="VBC28_001671"
     CDS             2898..3785
                     /locus_tag="VBC28_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEO6815637.1"
                     /translation="MAHTPTSHRKPRKRSPWLAIASVFFLLIALIGIFLFFNNRSKQE
                     IKTKTNASSHRKIVTSIKKKKWVKQKTPVKIPILMYHAVHVMDPSEAASANLIVAPDI
                     FESHIKRLKKEGYYFLAPNEAYRALNENALPEKKVIWITFDDGNADFYTKAYPILKKY
                     KVKATNNIITGFVQEGRESNLNVQQMLEMKQNGMSFQGHTVTHPNLSLLTPELQTQEM
                     TLSKLFLDQKLSQDTLAIAYPSGRYNPTTLDIASQYYKLGLTTNEGVATKDNGLLSLN
                     RVRILPTTSDDDLIKTINQ"
     gene            complement(3808..5355)
                     /locus_tag="VBC28_001672"
                     /pseudo
     CDS             complement(3808..5355)
                     /locus_tag="VBC28_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037479.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
     gene            complement(5498..6874)
                     /locus_tag="VBC28_001673"
     CDS             complement(5498..6874)
                     /locus_tag="VBC28_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEO6815638.1"
                     /translation="MAYKTIYPYTNEVLHEFDNISDSDLEQSLDIAHALYKTWRKEDN
                     VEERQNQLHKVADLLRKDRDKYAEVMTKDMGKLFTEAQGEVDLCADIADYYADNGQKF
                     LKPVPLESPNGEAYYLKQAVGVLLAVEPWNFPFYQIMRVFAPNFIVGNTMLLKHASIC
                     PASAQAFEDLVREAGAPEGAFKNIFASHDQVSNLISDPRVAGVCLTGSERGGASIAAE
                     AGKNLKKSSMELGGNDAFLILDDADFDLLSKTIFFARLYNAGQVCTSSKRFIVMADKY
                     DEFVNMVVETFKSAKWGDPMDSETTLAPLSSAGAKDDVLKQIKLAVDHGAEVVFGNDT
                     IDHPGNFVMPTVLTNITKANPIYNQEIFGPVASIYKVDTEEEAIALANDSSYGLGSTV
                     FSSDPEHAKKVAAQIETGMTFINSGWTSLPELPFGGIKNSGYGRELSQLGFDAFVNEH
                     LVFTPNSD"
     gene            complement(6948..7955)
                     /locus_tag="VBC28_001674"
     CDS             complement(6948..7955)
                     /locus_tag="VBC28_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family putative sulfate export transporter"
                     /protein_id="HEO6815639.1"
                     /translation="MLFKEKIPGLILCFIIAIPSWLLGLYLPLIGAPVFAILIGIIVG
                     SFYQNRQLFNKGIAFTSKYILQTAVVLLGFGLNLMQVMKVGISSLPIIIMTISISLII
                     AYVLQKLFKLDKTIATLIGVGSSICGGSAIAATAPVINAKDDEVAQAISVIFLFNILA
                     ALIFPTLGNFIGLSDHGFALFAGTAVNDTSSVTATATAWDAINHSNTLGGATIVKLTR
                     TLAIIPITIVLSIYHMKQTQKEQSVSVTKIFPKFVLYFILASLLTTIVASLGFSLRIF
                     EPLKVLSKFFIVMAMGAIGINTNVSKLIKTGGKSILLGAACWLGIIIVSLTMQAILGT
                     W"
     gene            complement(8063..8749)
                     /locus_tag="VBC28_001675"
     CDS             complement(8063..8749)
                     /locus_tag="VBC28_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6815640.1"
                     /translation="MKKFLKVFLTVLVLILIVGAGGLYFWNNRQSLEGKWRTVSLEKQ
                     VEKEIEQQLGSQVADMGISAADLVKGANMHMNVENDEAKITVTAQIDEVKFHQAIKTF
                     IDKALEKQLKDQGLTYNDLSEAGKKIFDETKITDKQIDQQIDRSFQSAAQAVGGKYNT
                     NTGEMTLPVMDGKVHRLTSVIKVSHINKKANAFYGNIVKNGEKTAYKKEGSKLILGNE
                     KSYPFMKVTK"
BASE COUNT         3015 a         1812 c         1324 g         2654 t
ORIGIN      
        1 aaatgagtgc aaacggagca ataaaactac tttctaccag catcgaaaaa tcgctagata
       61 aagaaattga tttttttaat aacccataaa aaccaaaaga aagcgctaga gttagtgaaa
      121 ttaaaggaaa atgaccagtt tgataaacta aaattccaac tcccatgatt gcaatcaaaa
      181 ttgcaaggct aactactctt gccaggtgtt cacgtaaaac aaggaccgac aacaaaattg
      241 aaataatcgg catcatataa taaccaagac ttgcctcagt agcgtgacca tgcgtgactg
      301 caaaaatata gaccaaccaa ttaattgaaa ttaaaatact ggctacaaac attgtccaaa
      361 atgacttttt attattaact aagcctttta agtcttttaa atagactgtt tttctacccg
      421 aaacgagcat ataaataagc atagttaaaa cagtgaatat aatcctataa gcaaacgtac
      481 tatatgcttc aattccagat aataatttcc aataaagaga taaaagcccc cataaaacat
      541 aggctgacaa gccaaggata attcctaaat tatcttttct caactctcac tccttttcgc
      601 tcaatattaa gtacttgaat ctgtccatct aaacataatt tacttaactc tgtgacaatt
      661 ggttgttctt tttctttcga acacagaacc attaccgtcg gtcctgctcc agacaaataa
      721 gtagcatacg caccgttttg agtgctgatt tgttttataa ctccaaattc cttcaccaaa
      781 ggttgacgat atctttcatg aaagaggtcc ctttctatag cccatccagc agttaccaaa
      841 tcacctttca acaacgacgc aaccatcaca ttagcgacgc tgcttgaagc aactgcatcc
      901 tcataactca atttttgagg tagtacattt cgactatcac ttgtttttaa ttcataattt
      961 ggaataaaag cgataaagga actatctgga aacatcactt ccaaactttt aatatcattt
     1021 ttcactattg atgaaataac taaatcacca aaaatggccg gtgctacatt atcaggatgt
     1081 ccttctaatc gggtagctat ctctaatttt tctttttgag aaagagccaa atttcctaat
     1141 tgattggcta attctattcc tgcaacaatt acagaggaag aagaacctaa acctctggcc
     1201 aaaggaatat ctgaaaacat ttttaagcgg tgtggtgcca aatccgactt aacatgcaaa
     1261 gctgtttgaa taaggaggtt agtatgatct tttggaatat ttactaaatt gtgttcaact
     1321 aaccactctg tactttcttc taatacttca atgcttaaat actttgataa cgcaactcct
     1381 attgaatcaa agcctggacc aatattcgca ctagttgcag gtacaattat cctcatttca
     1441 ccctcctatt actttgaaaa cattgattaa ctgaatgaca ccactctcag gaaactcctc
     1501 aataaattga gttattttct cttggctaac ttcatctgtt aatacataga tttcttgttg
     1561 tttatcatcc aatgcttttt caataacatt tttcaaacct atattttctt gctcaaagta
     1621 ccttccaagt gtatcactat cttcagcatt tctcaaagaa atagctagat agtactcatt
     1681 gaagatatca gactgcattg acaacctaca cggacaatga taagtattca taggcttaat
     1741 ttttatctgt gatcgaatac tacgactaat atcgatgata tcggctaaaa cagaggttgc
     1801 tgttggattt tgtcccgcac ctggtccata aaaaagagac tgaccaatcc ctattgattc
     1861 aatgaatact gcattcatca catgattaac tgcagctaag ggatgctctt gagaaattag
     1921 tgtcggagaa acgtctacac ttataccagt tggagtttca cgaagatcag ctaccaactt
     1981 aatggcaaag cccatctcct gagctacttc aacgtcctct gagcgaatac tagaaatccc
     2041 cttataatta acatcatcaa aatcaatagg cataccaaat ccaaattgac ttaagatagt
     2101 ggctttgtag gcagtatcaa taccttcaac atcatttgta ggatcacttt cggcataacc
     2161 taactcttgt gcctttttta gagcttgttc ataagaccat ccctcttcaa acatttttgt
     2221 taacatgaag ttggaggtac cgttgagaat tcctaataaa cgtgtcattt tatctgaggc
     2281 aaacgaatgc gatagggtcc ttaaaatagg aatgcctcca gcaactgctc cctcatagaa
     2341 taaagctaga cctctagcgt ctgctaaatc catcaactct tttccgtgta aagcaatgag
     2401 atctttattg gctgtcacaa tatgtttacc aaatcctaat gcttgactca aataagtttt
     2461 agctggctca attcccccca ttaattccac tacaatatca atttgtggat cttgaagaat
     2521 agtatttatc tctgtcacaa agttataagt aaatccctga ttgatgaaac gctggcggct
     2581 ttcattatct cttactaata ctttatcaat cacaatatct tcgccttcta aagaaagtag
     2641 cttatgttga ttttctttta gcaaatatgg aataccctta gcaaccgttc caaaacctaa
     2701 taaagctatt ttaatagtca tgaatttctc cttagaaatt gtctctaact tcaagacaaa
     2761 cattggctat attataacaa attaccaata ttttttatag ggtaaacgcg tcttgtccta
     2821 tgaaagttct taaaaagaga atattatttc agaaaagatg atacaataaa agtactttaa
     2881 aagttaagga gaaataaatg gcacacacac ctactagtca tcgtaaacca cgaaaacgtt
     2941 ccccatggtt agctattgct tctgtctttt ttttgttaat agctttaatc ggcatatttc
     3001 tattcttcaa caatcgttca aagcaagaaa taaaaacaaa aacgaacgcc tctagtcatc
     3061 gaaaaattgt cacatctatc aaaaaaaaga aatgggttaa gcaaaagact cctgttaaaa
     3121 tacccatttt aatgtaccat gctgttcatg ttatggaccc ttctgaagcc gccagtgcta
     3181 acttaattgt ggcacctgac atttttgaaa gtcatataaa acgtctcaaa aaggaaggat
     3241 attacttctt agcccctaac gaggcatatc gtgcactaaa cgaaaatgct ttacctgaaa
     3301 aaaaagtcat ttggattact tttgatgatg gaaatgccga tttctacact aaggcatacc
     3361 ctatactcaa aaagtacaag gttaaagcca ctaataatat catcactggc tttgttcaag
     3421 aaggacgaga atctaacctc aacgttcaac aaatgctaga aatgaagcaa aacggcatgt
     3481 cattccaagg acatacggtt actcacccca atctttctct cttaactcca gaactacaaa
     3541 cccaagaaat gacactatca aaactgtttt tagatcaaaa actctctcaa gatactcttg
     3601 cgattgctta tccatcagga cgctacaatc ctacaacttt ggatattgct agtcaatatt
     3661 ataaactagg gcttacaaca aacgaaggag ttgcaacaaa agataacggt ttgctttctc
     3721 ttaatcgagt tcgtatctta ccaactacta gcgatgatga tttgatcaaa acgattaacc
     3781 aataaaaaga agccttctgg cttcttttta ttcttcataa gatttaaatg gttcattttt
     3841 acgtggataa cgatctggag aaatcgataa gcccatttct tttttctcat ggaaaagctc
     3901 cactaatagc gctagcatca tcaaaatcat tacgaatgaa aatggcagag cgacaataat
     3961 taaaacgttc tgcagcgcta acagaccacc agacaagagt aagacaatag caatcactgc
     4021 caacactagc ccccaaatta ctttagttcg attttttgga tttaaattac catcttctgt
     4081 caacatagcc aaaacgtacg ttgcagaatc tgctgatgta ataaaaaatg aaaaaattaa
     4141 aatgatagca atagtggata aaagccaacc taaagtgtag tgattaaaag tagcaaacaa
     4201 tacttcctct gttgcaaact ttgtcaaatt aatacccagt tgttgtacct gagttgataa
     4261 tgtgccaaat gctgcaaacc atacaaaact caataatgtc ggtattaaaa gaactaccgt
     4321 taaaaactcc cgaatgctac gccctttaga aatacgagcg atgaaaactc caacaaaagg
     4381 agaccaggaa atccaccacg cccaataaaa aatcgtccaa ttatcaatcc aagaacgttt
     4441 agtattgtca aaagcagctg cacgaaagct cattccaaag aaattttgta aataagagcc
     4501 taaagactct gttagggtat caaaaatacg aaccgttggt cccaatacaa taactaaagc
     4561 taagagggct accgctaaaa tcaaatttaa gttcgataaa attttaacac cttttcctaa
     4621 acctgataaa gctgacataa caaataaagc tgttgtaata aggataataa caatttgaac
     4681 aaatgcatta ttgggaacac ccaataaaaa acttaaccct ccgttgattt gagcagctcc
     4741 aaatccaagt gtcgtagcaa ctccaataac tgtagcaaca actgtggtga tatcaacaat
     4801 ttttcctagt caaccatctg tcttatcacc gaaaagaggc tttaaagtga cagacaagag
     4861 gtatttctct tgctttcgaa atccaaaata agctagagcc aaagcaacca atgcatatac
     4921 tgcccaagca tgtatccccc agtgaaaaaa tgtaaaacga aatgcatcag ctaatgctgt
     4981 ttgcgattcc ttaggtgcgc caggtgtcga aatagcaaaa tgcgatgagg gttcagctgc
     5041 tccgtagaag accaaaccaa tccccatacc tgctgaaaac atcattgcta tccatgatac
     5101 agttgaatat tcaggacgtg aatgaggtcg ccctaaacgt atttgtccaa gaggactcat
     5161 aataaaaaag acacacaaag caagaattga agtaactaac aacaagtaca accaaccgaa
     5221 attggtagat acctgttcac gcaagatttg tgtccaagat tgaaaaccac gaggaacaat
     5281 aattcctaat aagactaaaa taagcgatac aattagtgaa cctgtaaaca caggcgtaat
     5341 atgtttttta ctcaaaattt cctcctatac taaaatagta tagcatactt taataaatac
     5401 tcctctcatt ttaatcacct gtatcaagac ctctccttgt ctcctcaaca aaaaagaacc
     5461 agataacatt atctggttct tgtgtttgac ttagtgatta atcactattt ggtgtaaata
     5521 ccaaatgttc gttgacaaag gcatcaaatc caagttggct caattcacga ccatatcctg
     5581 aatttttaat acctccaaac ggtaattccg gtaatgatgt ccaccctgaa ttaataaatg
     5641 tcatccctgt ttcaatctga gccgccactt ttttagcatg ttctggatca gaagagaaaa
     5701 cagtacttcc taaaccataa ctagaatcat tagctaaagc aatagcttct tcttcagtat
     5761 ccactttata gatagaggct acggggccga aaatttcttg attatagatt ggatttgctt
     5821 tagtgatatt cgttaaaaca gttggcatga cgaagtttcc tggatgatct atagtgtcat
     5881 ttccaaaaac tacctcagca ccgtggtcta ccgccaattt aatttgtttt aaaacatcat
     5941 cctttgcacc tgcagatgac agtggagcca aggttgtttc cgaatccatt gggtcacccc
     6001 atttagcaga cttaaaggtc tcaactacca tatttacaaa ttcgtcatac ttatcagcca
     6061 taacaatgaa acgttttgaa gatgtacata cttgacctgc attatataaa cgcgcaaaga
     6121 atattgtttt actaagtaaa tcaaaatcag catcatctaa aataagaaaa gcatcgttcc
     6181 cacctaattc catagacgat tttttcaaat ttttaccagc ctcagctgct attgatgccc
     6241 caccacgctc agaaccagtt aaacacacac ctgcaacacg aggatctgat ataaggtttg
     6301 aaacttgatc atgcgaagca aaaatatttt taaacgctcc ttctggagct cctgcttctc
     6361 ggactaaatc ctcaaatgct tgagctgatg caggacaaat agaagcatgt tttaaaagca
     6421 tagtattacc cactataaag ttaggggcaa atacacgcat aatctgataa aatgggaaat
     6481 tccatggctc gacagcaagt aaaacaccga cagcttgttt caaatagtaa gcctctccat
     6541 ttggactttc taacggaaca ggttttaaaa acttttgccc attatcagca taatagtcag
     6601 ctatgtctgc acacaagtcc acttctcctt gtgcttcagt gaaaagtttt cccatatctt
     6661 tagtcataac ttcagcgtac ttatcacggt ccttacgtaa taaatctgct actttatgca
     6721 attgattttg gcgttcttca acattatctt ctttgcgcca tgtcttgtag agggcatgcg
     6781 ctatgtctaa tgactgttcg agatcactgt cactgatatt atcgaattca tgcaatactt
     6841 cgttagtgta cggataaatt gttttatatg ccataatgtt ccctcctttt gatatcctca
     6901 ttataacatt tatgaaatcg attacaaata tttagcttta gacaagatca ccaagttcca
     6961 aggatagctt gcattgttaa actaactata ataatgccta accaacacgc tgcacctaaa
     7021 agaattgatt ttcctccagt tttaattagt ttgctgacat tagtatttat accaattgcc
     7081 cccattgcca taacaataaa aaatttagat aaaaccttaa gtggctcaaa gatacgtaac
     7141 gaaaaaccta atgaagcaac tatcgttgtt agcaaagaag ctaagataaa ataaagcaca
     7201 aacttcggaa atatcttagt aacacttaca gattgttctt tctgcgtttg tttcatatga
     7261 taaatagata atacaatcgt aattggaatg atggctaatg ttcgtgtcaa tttaacaata
     7321 gtagctccgc ccaaagtatt gctgtgattg atagcatccc atgcagtagc agttgctgta
     7381 actgacgagg tatcattgac agcagtccct gcaaataatg caaaaccgtg atcagagaga
     7441 ccaataaaat tccccagcgt tggaaaaatt aaagccgcta agatattgaa taaaaaaata
     7501 accgaaatag cttgtgctac ttcatcatct ttagcattaa taactggtgc agtcgctgca
     7561 attgcggatc caccacaaat agatgaacca acacctatta aagtagcaat cgttttatct
     7621 aacttaaata acttttgcaa aacataagca ataatcaatg aaatggaaat cgtcataata
     7681 atgatgggta acgatgatat accaactttc atcacttgca tgaggtttag tccaaaaccc
     7741 aataagacaa ctgctgtttg taaaatatat ttagacgtaa aagcaatacc cttattaaac
     7801 aattgtcgat tttgataaaa ggatccaaca attattccaa tcaagatagc aaagactggt
     7861 gctcctatta aagggagata aagcccaagc aaccaagatg gtatagcaat aataaagcat
     7921 aatattagtc caggaatttt ttccttaaat aacatgatag tcctcctcac attgactatt
     7981 atatcacaag ctttaaaacg tggcaggaaa accgaaagag gaccagaata tgctctgatc
     8041 ctctttcatt actaattcac aattatttag ttactttcat aaatggatat gatttttcgt
     8101 tacctaaaat caattttgag ccctcttttt tataggctgt tttttcacca tttttcacaa
     8161 tattaccata gaacgcattt gcctttttat tgatatgcga tactttaata acacttgtta
     8221 agcgatgtac tttaccatcc ataactggca atgtcatttc accagtatta gtattatatt
     8281 ttcctccgac agcttgtgca gcactttgga aacttctatc aatttgttgg tcaatttgtt
     8341 tgtcagtgat ttttgtttca tcaaaaatct ttttacctgc ttcacttaaa tcattgtaag
     8401 tcaaaccttg atcttttaat tgtttttcaa gagctttatc aataaaagtt tttatagctt
     8461 gatggaattt tacctcatca atttgagcag tgactgttat ctttgcctca tcgttttcga
     8521 cgttcatatg catattagcg cccttaacca aatcagctgc actaatcccc atatccgcta
     8581 cctgacttcc aagttgttgt tctatttctt tctcaacttg cttctctaaa gaaactgtac
     8641 gccatttccc ctctagtgac tgacgattat tccagaaata gagaccgcct gcacctacaa
     8701 ttagaataag tactagtaca gttaaaaaaa cttttaaaaa ctttttcatg ataaaaactc
     8761 cttatgataa actacattaa tcataacata atatcacaac tcctt
//
LOCUS       DATRMA010000075         8469 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.075, whole genome shotgun sequence.
ACCESSION   DATRMA010000075 DATRMA010000000
VERSION     DATRMA010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8469
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.075"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            127..2760
                     /locus_tag="VBC28_001676"
     CDS             127..2760
                     /locus_tag="VBC28_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HEO6815641.1"
                     /translation="MKGQKIIALAGLVLSCHFALTACHTQEHKNSHHIKTKQVAKKKA
                     NKKKVSVKESHKKRKGVAGVDFPTDDGFLLTKDSKILSHTDSGIVVAHGNHSHFIFYS
                     DLKGSKFSYLIPNANTKTNKNQAVRNFKAGAVAVNTLNDGYVFNPADIVSEDANGYVV
                     RHGDHFHYIPKASLSQQKQVQASRAVSRLGNQSNSHYRVNSSKIAGLHYPTSNGFLFN
                     GRGIKGTTPTGILVEHHNHLHFISFADLRKGGWGSIADRYQPQKKADSKKQSPSSKKP
                     RTENTLPKDIKDKLAYLARELHLDISRIRVLKTLNGEIGFEYPHDDHTHVIMAKDIDL
                     SKPIPNPHHDDEDHHKGHHHDESDHKHEEHEHTKSNKLSDEDQKKLIYLAEKLGLNPN
                     QIEVLTSEDGSIIFKYPHDDHSHTIASKDIEIGKPIPDGHHDHSHAKDKVGMATLKQI
                     GFDDEIIQDILHADAPTPFPSNETNPEKMRQWLATVTKINIGQRTNPFQRFGLSLMPN
                     IEVLGIGFTPINDMTPVLQFKKLKQLWMTNTGITDYSFLDKMPLLEGLDISQNGIKDL
                     SFLTKYKQLSLIAAANNGITSLKPLAELPNLQFLVLSHNNISDLTPLSNLTKLQELHL
                     DHNNVKNLSALSGKKDLKVLDLSNNKSADLSTLKTTSLETLLLNETNTSNLSFLKQNP
                     KVSNLTINNAKLASLDCIEESDEIVKVEAEGNQIKSLVLKNKQGSLKFLNVTNNQLTS
                     LEGVNNYTSLETLSVSKNKLESLDIKTPNKTVTNLDFSHNNVPTSQLKLNEKNIPEAV
                     AKNFPAVVEGSMVGNGSLAEKAAMASKEDKQVSDNTNHQKNTEKSAQANADSKKENPK
                     THDEHHDHEETDHAHVGHHHH"
     gene            2860..3519
                     /locus_tag="VBC28_001677"
     CDS             2860..3519
                     /locus_tag="VBC28_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001884604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEO6815642.1"
                     /translation="MTMVKREDVILNATENMVHHKLKNDPSGHDWFHIVRVRNLAVEL
                     AHKEGANTFICQMAALLHDIIDDKICQDSKQASYELTQWLYSQDLAIAEVEHILDILE
                     NISFKAGTGLTMKTLEGQIVQDADRLDAMGAIGIARTMAYSGSKGRLIHDPNLKPREN
                     LTLEEYRNGQDTAIMHFYEKLLKLKDLMNTKQGKMLAQKRHDFLELYLAEFYAEWNGK
                     R"
     gene            3528..3992
                     /locus_tag="VBC28_001678"
     CDS             3528..3992
                     /locus_tag="VBC28_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000621138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO6815643.1"
                     /translation="MIRRAKEKDLPDIAELLKQILMLHHEVRPDIFHTRGSKFSKEQL
                     KEMLIDESKPIFVYESDEGKVVAHLFLQLQEKRDLPRKSFKTLYIDDLCIDEEVRGQQ
                     IGQKLMDFARQYAKKHGCYNITLNVWNDNQRAVSFYEKLGFKPQQTQMEEIL"
     gene            3992..4426
                     /gene="msrB"
                     /locus_tag="VBC28_001679"
     CDS             3992..4426
                     /gene="msrB"
                     /locus_tag="VBC28_001679"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEO6815644.1"
                     /translation="MKETQEELRQRIGHTAYQVTQNSATEHAFTGKYDDFFEEGIYVD
                     IVSGEVLFSSLDKFQSGCGWPAFSKPIENRMVTNHQDHSHGMHRIEVRSRQADSHLGH
                     VFNDGPVDAGGLRYCINSAALDFIPYDQMAKRGYGDYLSLFD"
     gene            4578..7370
                     /locus_tag="VBC28_001680"
     CDS             4578..7370
                     /locus_tag="VBC28_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HEO6815645.1"
                     /translation="MASTNNVSDTNTRFAKEELEEVFEELGTTQGGLSDEEVAVRQKK
                     YGLNLLSEVKQESIILLFLKNFTSLMAILLWVGGFVAIVSNSLELGLAIWMVNVINGI
                     FSFIQEYRASQATQALEKMLPSYSRVLRKGSEEKILSEQLVPGDIVLIEEGDRISADG
                     RLIKTTDLQVNQSALTGESNPIYKDSNVENDQSKTLIECDNMVFAGTTVSSGSATMVV
                     TAIGMQTQFGQIADLTQGMKSEKSPLQRELDRLTKQISIISITVGIIFFLAATFFVKE
                     PVSKSFIFALGMIVAFIPEGLLPTVTLSLAMAVQRMAKEHALVKKLSSVETLGATSVI
                     CSDKTGTLTQNEMTVNHLWQNGKSYQVTGLGYAPEGQILFEGDNICFGNSDRGDLEKL
                     IRFAHLCSNAQVLPPNDDRSTYTVLGDPTEACLNVLLEKSGINIQENRKFAPRLKELP
                     FDSVRKRMTTIHSLGGDEKDKKISITKGAPKEILDLSDYVLSDGKVIPLNKEERNKIQ
                     LANDTFAKDGLRVLAVSYCDIEGFSKEQWTQENLEQHMVFIGLIAMSDPPREGVREAI
                     DKCHAASIRIIMVTGDYGLTALSIAKNIGIIRNDDAKVISGLELSEMTDSQLKKELSG
                     EVVFARVAPEQKYRVVTILQEMGEVVAVTGDGVNDAPALKKSDIGVAMGVTGTDVAKE
                     SADMILTDDHFASIVHAVEEGRAVYQNIKKFLTYIFNSNTPEAVPSAFFLFSKGFIPL
                     PLTVMQILAVDLGTDMLPALGLGVEPPETDVMNRPPRRLTDRLLDKGLLIKSFLWYGT
                     IESVLAMGGFFWAHYLRYGNFTFFAANGIPYREATTMTLGAIIFSQIGMVMNSRTSYQ
                     SIKALSIFGNKLINFGIIMEILAFLVLVYVPLFHNLFNTASLGLSHWLYLISCPFIMI
                     GLDEVRKLFSSRKNKR"
     gene            7370..>8469
                     /locus_tag="VBC28_001681"
     CDS             7370..>8469
                     /locus_tag="VBC28_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2974 domain-containing protein"
                     /protein_id="HEO6815646.1"
                     /translation="MSNIITYLKNNSNLTFDELALNDVDILCLNEFGYISFEKLINTT
                     EMKSVLVCELYHEYLQTMAKSYSFMFTSQRHDLCQLMMTSKRFKNLTLSYYRAEISLE
                     FEKQFAAMVFTIPNINYHQVVFRGTDANLIGWKEDFKLTYMREISAHRSAIKYLNTIL
                     PYFDKVVLSGHSKGGNLALYAAMFTKPDLKAKIDLIWLIDSPGLQKTLLPTTEYKTTK
                     QKCIRLLPEESIVGMMLYSDIEPLIISSNARGILQHDVTTWEIQEPAILKTGTGLSLK
                     SICFEKTFQQWMAELKSQERKLFFDLLFDSFLSSGVSSLDDFNLASRAKMMKAFHSFR
                     ELDDDKKRLFNKSLKLLVTIFWGAYHDNSRET"
BASE COUNT         2947 a         1328 c         1603 g         2591 t
ORIGIN      
        1 tgagctataa atgagttttt gttagtaact aaataataaa aagatggcaa atagtattgc
       61 aaaaagaaaa acatctgata taattaacta gttaactaaa acgaaaaaaa catttatgga
      121 ggactcatga aaggtcaaaa aattattgct ctagctggtc ttgtcctgtc atgccatttt
      181 gctcttacgg catgtcatac tcaagaacac aaaaattctc atcatattaa aacaaagcag
      241 gttgctaaga aaaaagctaa taaaaagaaa gtctctgtta aggaaagtca taaaaaacgc
      301 aagggtgttg caggggttga ctttccaact gatgatggct ttttactgac taaagattcg
      361 aagattttat ctcatacaga ttctggaatc gttgtagcac atggaaatca ttctcatttt
      421 attttttact ctgatttaaa aggttcaaaa tttagctatt taatccctaa tgctaataca
      481 aaaactaata aaaatcaagc ggtaagaaac tttaaagcag gggcggtagc tgttaataca
      541 ttaaatgatg gctatgtttt taatcccgca gatattgttt cagaagatgc taatggctac
      601 gtggtcagac atggtgatca tttccactat attccaaaag caagtttatc tcagcaaaag
      661 caagtacaag caagtagagc tgtttcacgt ttaggaaatc aaagtaatag ccattacagg
      721 gttaatagtt ctaaaattgc agggcttcac tacccaacaa gtaatggttt cttatttaat
      781 ggacgaggaa taaaaggcac taccccaaca ggtattttag tagaacatca taatcatctg
      841 cattttatta gttttgctga tttaagaaaa ggggggtggg gatcaattgc tgaccgttac
      901 caaccacaaa agaaagctga ttctaaaaaa cagtcaccat caagtaaaaa gccaagaact
      961 gaaaatactt tacctaagga catcaaagat aagctagctt accttgctcg tgaattacat
     1021 ttagatattt cacgtatcag agtccttaaa acactaaatg gtgaaattgg atttgaatac
     1081 ccacatgatg atcatacaca tgttattatg gccaaagata ttgatctaag taagccaatt
     1141 ccaaatccac atcatgatga tgaagatcat cacaaaggtc atcaccatga tgagtccgac
     1201 cataagcatg aagaacacga gcacactaag tcaaataaat tatctgatga agatcaaaag
     1261 aaattaattt atttagcaga gaagcttggt ttaaatccta atcaaattga agtattaacg
     1321 tcagaggatg gaagtatcat ctttaagtat ccacatgatg accactcaca caccatagca
     1381 agtaaggata ttgaaattgg gaagcctatc ccagatggac accacgatca ctctcatgca
     1441 aaagataaag ttggtatggc aactctaaaa caaattggtt ttgatgatga gattattcag
     1501 gatattctgc atgcagatgc tccaacacca ttcccatcaa atgaaactaa tcctgaaaaa
     1561 atgcgtcagt ggcttgcaac tgttactaag attaacattg gacaaagaac caatccattc
     1621 cagcgttttg gtctatcatt gatgcctaat attgaagttc taggaattgg gtttactccg
     1681 attaatgata tgacgccagt tttgcaattc aaaaaactta aacagctttg gatgacaaat
     1741 actggtatta cagattattc tttccttgat aagatgccac tattagaagg attggatatt
     1801 tcacaaaacg gtattaagga tttatctttc cttacaaaat ataaacaatt aagtcttatt
     1861 gctgcagcta ataatggcat tacttcgcta aaaccattgg ctgaattacc aaaccttcaa
     1921 ttcttagttt tgagtcataa caatatctct gacttgacac cgttatcaaa cttgacaaaa
     1981 ctccaagaat tgcaccttga tcataataat gtgaagaatt taagtgcact ttctggtaaa
     2041 aaagatttga aagttctaga tttatcaaac aataaatccg cagacttatc gactctaaaa
     2101 acaactagtc ttgagacgct tcttttaaat gagacgaata caagcaatct aagtttctta
     2161 aagcaaaatc ctaaggtatc taatttaaca attaataatg cgaaattagc atcattagat
     2221 tgtattgaag agagcgatga aattgttaaa gtagaagctg aagggaacca aattaagtca
     2281 ctagtattga aaaataaaca aggttcgtta aaattcttga atgtgactaa taatcagtta
     2341 acatcacttg aaggtgttaa taattatact tcacttgaga ccttaagtgt ttctaaaaat
     2401 aaacttgaat ctttagatat taaaacacct aacaaaacag ttacaaatct tgattttagc
     2461 cataacaatg ttccaacatc acaattgaaa ttgaatgaaa aaaatattcc tgaagcagta
     2521 gctaagaatt ttccagcagt tgtagaaggt tcaatggttg gaaatggtag tcttgctgaa
     2581 aaagcagcta tggcttctaa agaggacaaa caagtttcag ataatactaa tcaccaaaaa
     2641 aacactgaaa aatctgctca agcgaatgct gatagtaaaa aagaaaaccc taaaacacat
     2701 gatgaacatc atgaccatga agaaacagat catgcacatg taggtcatca tcaccattaa
     2761 tcttttacag agctaatata taaaatcctt caagaatttc ttggaggatt ttgcatttat
     2821 atgctaaatc atttattttt ttagaaaatt agctataata tgactatggt taaaagagaa
     2881 gatgtgattt taaatgcaac tgaaaatatg gttcaccata aattaaaaaa tgatccaagt
     2941 ggccatgact ggtttcatat tgttagagta agaaatcttg cggtagaatt agcacacaaa
     3001 gaaggggcta atacctttat ttgtcaaatg gctgcacttt tgcatgatat tattgatgac
     3061 aaaatctgcc aagatagtaa gcaagcgtcg tatgaattga ctcaatggtt atatagtcaa
     3121 gatcttgcca tagcagaagt agaacatatc cttgatattc ttgaaaatat ttcattcaaa
     3181 gcaggaactg gattgactat gaaaacattg gaaggtcaaa ttgttcaaga tgcagatcgc
     3241 ctagatgcta tgggggctat tggtattgca agaacgatgg cttattcagg gagtaaagga
     3301 cgtttaattc atgaccctaa cttaaaacca cgagaaaacc tcactttaga agagtatcgt
     3361 aatggtcagg atacagctat catgcatttt tacgaaaaat tattaaagtt aaaagacctt
     3421 atgaatacaa aacaagggaa aatgcttgct caaaaacgtc atgatttttt agaactatat
     3481 ttagccgaat tttatgctga atggaatggt aaacgttagg agtagatatg attcgtagag
     3541 cgaaagaaaa agatttgcct gatattgcag agctattgaa acaaatttta atgttacatc
     3601 atgaggttcg cccagatatt tttcatacaa gaggaagtaa gttttctaaa gaacaattga
     3661 aagaaatgct gatagacgaa tcaaagccta tctttgttta tgaatcagac gagggtaaag
     3721 ttgttgccca cttgtttctt caattacaag aaaaacgtga cttacctcga aagtcattta
     3781 aaactctcta tatagatgat ttgtgcattg atgaggaagt aagaggtcag caaattggac
     3841 aaaagttgat ggattttgca agacaatatg ctaagaaaca tgggtgttat aatataactt
     3901 taaatgtttg gaatgataat cagagagcgg tctcttttta tgaaaaatta ggttttaaac
     3961 ctcaacaaac tcaaatggag gagatactat aatgaaagaa acacaagaag aattacgtca
     4021 gcgtattgga catacagctt atcaagtaac ccaaaattca gctacggaac atgcttttac
     4081 tgggaagtat gatgattttt ttgaagaggg gatatatgta gatatcgtca gtggagaggt
     4141 tttattctct tctttagata aatttcaatc tggttgtggt tggccagcat tttcaaaacc
     4201 aattgagaat cgaatggtga caaatcatca agatcactct catggaatgc atcgtattga
     4261 ggttaggagt cgtcaagctg actctcattt agggcatgta tttaatgatg gaccggttga
     4321 tgcaggtggt ttacgctatt gtatcaattc agctgcctta gattttattc cttatgacca
     4381 gatggcaaag agaggctatg gggactacct atcacttttt gattaataag agacgactta
     4441 agggtatatc ttaagtcgtt ttttatgtat ggtaaaaaga attggtggca ggtgtattct
     4501 tcatcagttt attttctgaa aaaagagtat aatataggta tcaaaaatga aaacgtttta
     4561 gagaaggaga ctagaatatg gcttctacaa ataatgtttc agatacaaac acacgttttg
     4621 ctaaagagga gttagaagag gttttcgaag aattagggac aactcaaggt ggtttatcag
     4681 atgaagaggt tgctgtacgt cagaaaaaat atggtttaaa cctgttatct gaagtaaagc
     4741 aagaatcgat tattttattg tttctgaaaa attttactag tcttatggca atactattat
     4801 gggttggagg atttgtggct attgtttcta atagcttaga gctaggtctt gccatttgga
     4861 tggttaatgt gattaatggt atttttagtt tcattcagga ataccgtgca agccaagcaa
     4921 cacaagctct cgagaaaatg ttaccatctt attcacgtgt tcttagaaaa ggctcagaag
     4981 aaaagatatt atcagagcaa ctagtcccag gcgatattgt tcttattgaa gaaggagacc
     5041 gtatttcagc agatggtcgc ttgattaaaa caacagattt gcaagtaaac caatcagcct
     5101 tgacagggga atcaaatcct atttataaag atagcaatgt tgaaaacgac cagtctaaaa
     5161 cattgattga atgtgataat atggttttcg caggaacgac ggtctccagt ggtagcgcaa
     5221 caatggttgt tacggcaatt ggaatgcaaa ctcaatttgg tcaaatcgct gatttaacac
     5281 aagggatgaa atcagaaaaa agccctcttc aaagagaact tgatcggttg accaaacaga
     5341 tttctatcat ttcaattacg gtaggaatta tctttttctt agcagcgact ttttttgtta
     5401 aggagccagt gtcaaaatca tttatctttg ccttggggat gatcgttgca tttatcccag
     5461 aaggtttact acctaccgtt acactttctc tagctatggc agtccaacga atggctaaag
     5521 aacacgcact tgtcaaaaaa ctttcttcag ttgagactct tggagcaacc tcagttatct
     5581 gttctgataa gactggtaca ttaacgcaaa atgaaatgac cgtcaatcat ttgtggcaaa
     5641 atggaaagag ttaccaagtg acaggactgg gttatgctcc agaaggacag attctttttg
     5701 aaggtgacaa tatttgcttt ggtaatagtg atagaggaga cttagaaaaa cttattcgtt
     5761 ttgctcatct ctgtagcaac gctcaagtat taccaccaaa tgatgatcgt tcaacttata
     5821 ctgtacttgg agatccgaca gaagcttgtt tgaatgtttt attggaaaaa tcagggataa
     5881 acatacaaga aaatcgtaag tttgcaccac gtttgaaaga attacctttt gattcggtta
     5941 gaaaacgcat gacaactatt catagcttag gaggcgatga aaaggataaa aaaatttcaa
     6001 ttacaaaagg tgcgccgaaa gaaatacttg atttatcaga ttatgtctta tctgatggca
     6061 aagttatccc cttaaacaag gaagaacgta ataaaataca gttggctaac gatacatttg
     6121 ctaaagatgg acttcgcgtc ttagctgttt catactgcga tattgaaggc ttttcaaaag
     6181 aacaatggac gcaagaaaat ttggaacaac acatggtgtt tatagggttg atagcaatga
     6241 gtgatccacc tcgtgaaggc gttcgcgaag ctattgataa atgtcatgca gcaagcattc
     6301 gtattattat ggtgactgga gattacggat taacggcact aagtatcgct aaaaatattg
     6361 gtattattcg taacgatgat gctaaagtaa tttcaggttt agagttatca gaaatgacgg
     6421 atagccaatt gaaaaaagaa ttgtcaggtg aagtcgtttt tgctcgtgta gcaccagaac
     6481 aaaaatatcg agtagtaacg atattacagg agatgggtga agtagtagca gttacaggag
     6541 acggggtcaa tgatgcgcct gcgctaaaaa aatccgatat tggtgtagcc atgggtgtta
     6601 ccggtactga tgttgctaaa gaatcagctg atatgattct gacagatgat cattttgctt
     6661 ctattgtaca tgctgtggaa gaaggacggg ctgtttatca aaacattaag aaattcttga
     6721 cgtatatttt taatagtaat actcctgagg ctgttccgtc ggcattcttc cttttttcta
     6781 aaggttttat tcctttacca ttgactgtaa tgcaaatact tgctgttgat ttgggaaccg
     6841 atatgcttcc cgctttaggt ctgggcgtgg aaccaccaga aacagacgtt atgaatcgtc
     6901 ctccaagacg gctaacagat cgcttattgg acaaagggct acttataaaa tctttccttt
     6961 ggtacggcac tattgaatcc gtattagcta tgggtggttt tttctgggct cattacttac
     7021 gttatggtaa ttttactttt ttcgccgcaa acggcatacc gtacagagaa gcgacaacta
     7081 tgacacttgg agctattatc tttagccaga taggtatggt tatgaatagc agaacgagct
     7141 atcaatctat taaagcacta tctatttttg gtaataaatt aattaatttt ggtataataa
     7201 tggagatttt agcattttta gtcttagtat acgtgccact attccataac ttattcaaca
     7261 cagcaagcct aggtttatca cattggctct atttaataag ctgcccattt atcatgatag
     7321 gtttagatga agttcggaaa ttattcagca gtagaaagaa taaacggtga tgtcaaatat
     7381 aataacttat ttaaaaaata attcaaactt aacttttgat gaattagcat taaacgatgt
     7441 tgacattctt tgtttaaatg aatttggcta tatttctttt gaaaaactta taaacactac
     7501 agaaatgaag tcagttttag tctgtgagtt atatcatgag taccttcaaa ctatggctaa
     7561 atcatatagc tttatgttca catcacaacg acatgattta tgtcagttaa tgatgacctc
     7621 gaaacgtttt aaaaacttaa ctttatctta ttatcgagct gagattagtt tagaatttga
     7681 aaaacaattt gcagctatgg tttttactat tcctaacatt aattatcatc aggttgtatt
     7741 tcgtggtact gatgccaacc ttattggttg gaaagaagat tttaaattga cttatatgag
     7801 agaaatatcg gctcatcgtt cagcgattaa atacttaaat acaatactgc cttattttga
     7861 taaagtagtt ctctcaggtc attctaaggg gggtaattta gccttgtacg ctgcaatgtt
     7921 tacaaagcca gatttaaaag caaaaattga tttgatttgg cttattgact ccccaggact
     7981 acaaaaaact ttgttaccta ctacagagta taaaactact aaacaaaagt gcattcgctt
     8041 actaccagaa gaatctattg ttggtatgat gctttatagt gatatagagc cgctaattat
     8101 tagtagcaat gcgcgtggaa ttctgcaaca cgatgttaca acttgggaaa ttcaagaacc
     8161 agctatttta aagactggaa caggactatc gctaaaaagt atttgttttg aaaagacatt
     8221 tcaacagtgg atggctgaat taaaaagtca agaaagaaaa ttattttttg atttattatt
     8281 tgatagcttc ctatcaagtg gcgtatctag tttggatgac ttcaacttgg cttccagagc
     8341 aaaaatgatg aaagcttttc atagctttag agaactagat gatgataaaa aaaggttatt
     8401 taacaaatct ttaaaattat tagtaaccat attttgggga gcttatcatg ataatagcag
     8461 agagactaa
//
LOCUS       DATRMA010000076         8451 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.076, whole genome shotgun sequence.
ACCESSION   DATRMA010000076 DATRMA010000000
VERSION     DATRMA010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8451
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.076"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            4..246
                     /locus_tag="VBC28_001682"
     CDS             4..246
                     /locus_tag="VBC28_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EsaB/YukD family protein"
                     /protein_id="HEO6815647.1"
                     /translation="MNTHINITLDMGETSKDLRIPLKISVKQLVMELDSIFSRTERRT
                     KYQLRVINKGILLGENDILAHHPVTTGDRIRIEEFY"
     gene            246..1520
                     /gene="essB"
                     /locus_tag="VBC28_001683"
     CDS             246..1520
                     /gene="essB"
                     /locus_tag="VBC28_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EssB"
                     /protein_id="HEO6815648.1"
                     /translation="METDSFVFEEQDFQFEKGPDFWQLSLKRSKVGTQNLLQLQLLEI
                     HHSLLMPMTTAIDADTIQFQFQTEAHALFFESFKKETLSEKLRLALNVLDLDKALSLS
                     VNFILHPSNLFLTKNATAKIAYRSLPGIMRPEKFGPEEFLYQFKCFVFALLTQHDYIE
                     LYNGAISVIEVSDFLKSIYHAETIQAVRDIITIDYEQQVEVETHTLAKVSRAKYKLYK
                     YISVWLGALSTILLIPLVYLVFIHNPFKEKMLAADTSFIKVDYNQVINRLEHVKVSKL
                     PYTQKYELAYSYINGMSFSEEQREVILNNVTLKTDELYLDYWINIGRGLDDDAIDAAK
                     RLDDSDLVIYAIVQKMDQVRKDNSLSGKDREQKLSELQTDYDKYWKDRKTALTDEESK
                     SKNSNNHSTNSNKESSESSSTTASTSSKTKSR"
     gene            1527..2030
                     /locus_tag="VBC28_001684"
                     /pseudo
     CDS             1527..2030
                     /locus_tag="VBC28_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001921811.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
     gene            2059..5934
                     /gene="essC"
                     /locus_tag="VBC28_001685"
     CDS             2059..5934
                     /gene="essC"
                     /locus_tag="VBC28_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001867146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EssC"
                     /protein_id="HEO6815649.1"
                     /translation="MSAYQFYEGYPDFHRSPRIIYRSSEDKITVNAPGNEPNKSKDEL
                     LKLIVPPLVMIGVSILISIFRPRGIYIIATMSMALVTMIFSITGYFKNRKQYKQDLQE
                     RIDSYHDYLSDKSIELQKLAKEQKRGQHYHYPTIEGLQEMADTYHHRIYEKTPLHFDF
                     LYYRLGLGEVPTSYNIHYSQPERSGKKDPLENEGYNLYFNNRYIKNMPIVANLSHGPV
                     GYIGPRGLVLEQLQLMVNQLAFFHSYHDVQFITIVPEEEMDKWSWMRWLPHETLQDVN
                     VRGFVYNQRSRDQVLNSLNQILKLRRTQREDKSAKEGTLFSPHYVVIVTDEKLILDHV
                     IMEFFTEDPTELGCSLIFVQDVMSSLSENIKTIINIKDRNTGQLVIEEGELKETDFEL
                     DHFLEDYDKENISRRLAPLNHLQNLKSSIPEAVTFMEMYQAEEFEDLHVQERWISHAP
                     YKSSAVPLGLRGQDDIVYLNLHEKAHGPHGLVAGTTGSGKSEIIQSYILSLAVNFHPH
                     DVAFLLIDYKGGGMANLFKDLPHLLGTITNLDGAQSMRALVSINAELKRRQRLFAKAD
                     VNHINQYQKKYKLGEVSEPMPHLFLISDEFAELKSNQPEFMKELVSTARIGRSLGIHL
                     ILATQKPSGVVDDQIWSNSRFKLALKVADRGDSMEMLHTPDAAEITQAGRAYLQVGNN
                     EVYELFQSAWSGADYQPEKDDQGIEDHTIYSINDLGQYEILNDDLSGLDQAENIKEVP
                     TELDAIVENIQALTKEMGISDLPQPWLPPLSNQIAVTDLRKEGSVDLWSKAPSYKAVL
                     GFMDIPSQQAQEVAYHDFEDDGHLSIFAGPSMGKSTALQTVTMDLARHNSPEFLNLYL
                     FDFGTNGLLPLRRLPHVADFFTIDDDEKIAKFIARIKVEMSDRKKALSRYNVATAKLY
                     RQVSGETMPQILIVIDSYEGLREAQTPTNLEACFQNISRDGSSLGISLVISAGRTAAL
                     RSSLMANLKERIALKLTDDSESRTLVGRHQHIMEDIPGRGLIKRDDIEVLQVALSTEG
                     TETFDIINNIQNESDAMNSKWTGPRPKAIPIVPEELTFDDFMATDSVQADLSANRLPL
                     GLEMVDVESYSLALNRFKHMLYMSDSDESLEAVGSHIIKVLLNVPNYHNMIIDSLGEY
                     ESYQGQVRTYVGSDMIEDMADQLIYELQQRESQSEIAEWFIMIPDFESFVAKSNMKLE
                     QVQALLDNGPRYGLHLILGSSYNYVGTKIDAVPKYVRTTTQYVLAGMRLMDQTFLEKV
                     YNSKEIRPNKDEAYIHDRKTYQKLKLTID"
     gene            5948..6298
                     /locus_tag="VBC28_001686"
     CDS             5948..6298
                     /locus_tag="VBC28_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04197 family type VII secretion effector"
                     /protein_id="HEO6815650.1"
                     /translation="MAKIQSDASVASTCATAIQSGASGITAVGAPTKDSNSDYSGNSD
                     ASTYIDGEASSSSQIAEKINEFIALIHSTVNEFEAIDQKISQEIAANTKYTMPSNDSS
                     VISNPDFKPKYGLF"
     gene            6310..6672
                     /locus_tag="VBC28_001687"
     CDS             6310..6672
                     /locus_tag="VBC28_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6815651.1"
                     /translation="MTERTFEDIELDLKLFQIKLDNAENSKRLLQKLKNDVMELQIEL
                     LESLKLGDAYLTESEELEENNDFILTVNSETLSLEESYDNRINLVSKEIMDYENALDK
                     LYYEKQSLMQKSNERKGG"
     gene            6675..7589
                     /locus_tag="VBC28_001688"
     CDS             6675..7589
                     /locus_tag="VBC28_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6815652.1"
                     /translation="MVKVSVSSVGTQASTVAISMFSRVSALNDAITKLSSFAEAATLQ
                     GTAYSNAKSYATGTLTPMLQGMILFSETLSEKCTELQTLYVSICGDEDLDSVVLESKL
                     ASDRASLKIAEALLEHLNDDPEPSKSAISSTKSNIKKLKKRIKSNQKKLDNLNEFNAH
                     SATVFADISNAQSTVNQALAAVSTGFSGYNSKTGAFGKPTSGQMEWTKTVKKNWKERE
                     DAKAEELKSKKAEESKKASKIENTTKKSNVSVDKKKLIKAANEAYKLGEIKKDTYESI
                     ISGLSNASAALLKEVAKSKLTDTARLLM"
     gene            7718..8023
                     /locus_tag="VBC28_001689"
     CDS             7718..8023
                     /locus_tag="VBC28_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000847739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6815653.1"
                     /translation="MKTGAPVIIGSAAKVVGTAAGTATAGLLISTGVLAPPAPVVGSA
                     VDFGATAVIGVGGSMVFDYVYDNKDEINHIIKRASKDTINNVSDFFELMYDSTGSIN"
     gene            8037..>8451
                     /locus_tag="VBC28_001690"
     CDS             8037..>8451
                     /locus_tag="VBC28_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6815654.1"
                     /translation="MIHREKSYAEIYHDGNQGYYYNFDTKIFYIKSFETPNYQKENLE
                     NIKKYSSIITFLVLILIPINNWYRTIANLKLNLILAIMFIVIALVVVNKLNNIVSKQK
                     RNIDNISYEKMEFDKEKIANIIVSAEELSNKLLFLL"
BASE COUNT         2909 a         1447 c         1605 g         2490 t
ORIGIN      
        1 aggatgaata cacatattaa cattactcta gatatggggg aaacctcaaa agatttacgt
       61 atccctttga aaattagcgt taagcaatta gtaatggaat tagatagtat ttttagccgt
      121 actgaaaggc gtaccaaata tcaactaaga gttataaata aaggtatttt attgggagag
      181 aacgatatat tagcgcacca tccagtaaca actggtgatc gtataaggat agaggagttt
      241 tattaatgga aacagattcg tttgtatttg aagaacaaga ctttcaattt gaaaaaggac
      301 cagatttttg gcagttatct cttaagcgtt caaaagtagg aactcaaaat ctgctacaac
      361 tgcaattgtt agaaattcac cattctttgt tgatgcctat gacaactgca attgatgcag
      421 ataccattca atttcaattc cagacagaag cgcacgctct attttttgaa agctttaaaa
      481 aagaaacttt atctgaaaaa ttacgattag ccttgaatgt cttggattta gataaggctt
      541 tgtctttgtc agtaaatttc attttgcatc cctctaatct atttttaaca aaaaatgcaa
      601 ctgctaaaat agcttatcgt agccttcctg ggattatgag acctgagaaa tttggtccag
      661 aagagttttt atatcaattc aaatgttttg tctttgcatt attgacgcaa catgactata
      721 tagagttgta taatggtgct atttctgtta ttgaagtatc agatttccta aaaagcattt
      781 atcatgcaga aactattcag gctgttagag acatcattac tattgattat gagcagcaag
      841 tagaagtaga aactcatacg ttagcaaaag tatcaagggc aaaatataag ctttataaat
      901 acataagtgt ttggctagga gctttatcta caatactctt gattccgcta gtatatctgg
      961 tttttattca caatccattc aaagaaaaaa tgttggcagc ggatacttca tttattaaag
     1021 ttgattataa tcaagtgatt aatcgactag aacatgtaaa agtaagtaag ttaccttata
     1081 cacagaagta cgagttggcc tattcctata ttaatggaat gtcattttct gaagaacagc
     1141 gtgaagttat tttaaacaat gttacgctca agactgatga gctctatctt gattattgga
     1201 ttaatattgg ccgtggttta gatgatgatg ctattgatgc tgccaaacgt ttagacgatt
     1261 ctgaccttgt tatttatgct attgttcaga aaatggatca ggttagaaaa gacaatagtt
     1321 tatctggtaa agatcgtgaa caaaaacttt ctgagctaca aacagactat gataaatatt
     1381 ggaaagaccg taagacagcc ttaacagatg aggaatccaa atcgaaaaat agcaataatc
     1441 attcgacaaa ttccaacaaa gagtcatctg aatcatcaag tactacagca agtacatctt
     1501 ctaaaactaa aagtaggtag gtggatatgt tagttatttg ttattatcaa tcactccgct
     1561 acgaatttaa tattgaagag gagaaatcct ttctaatatc atcaaacggc aagtcgccaa
     1621 taccagtatc agatttagaa aatgacatca cgctaaaaaa tatacaaggt caattggtat
     1681 atattattga ctaaaaagaa aaagagttaa ctaatggtgt agagatttca ggaattgtct
     1741 tctatttagc gaataatcaa aaagaaattt atactccctt agattacgaa gatatattaa
     1801 tcggtgataa agaagggtat cgtgtccgtt ttaaagaagg agcccctaat ttattactga
     1861 aaaaaataga aagtaattgg cagcttaatt tatttgaagg tgatatttat ctgaataatc
     1921 atcttcaaaa agtagtgcag cagttacctt taagtttagg tgatgagatt agcttccaag
     1981 gaacaattgt taagcttttc ccagatgaaa ttcaaatctg ggggggctga ttatgaaaca
     2041 tcattaacta agaaagttat gtcagcttat caattttacg aaggctatcc tgattttcac
     2101 cgctcacctc gtattattta tcgttccagt gaagataaaa ttacagttaa tgcgcctggt
     2161 aatgaaccta ataagtctaa agatgaactg ttaaaattaa ttgttcctcc tctagtgatg
     2221 attggagtca gcattcttat ttctattttt cgtcctcgtg gtatctatat cattgcaaca
     2281 atgtcaatgg ctctagtgac gatgattttc tctatcactg gttactttaa aaatcgtaaa
     2341 cagtataagc aggacttaca agaacgcatt gattcctatc atgactattt atctgataag
     2401 tcaattgaac ttcagaagtt agctaaagag caaaagcgtg ggcaacacta tcattaccca
     2461 acgattgaag gtttacagga aatggctgat acttatcacc atcgtattta tgaaaagaca
     2521 ccacttcatt ttgacttctt gtattatcgc ctaggtttgg gagaagtacc aacgagctat
     2581 aatattcatt attctcaacc agaacgttct gggaaaaaag atcctttaga gaacgaaggc
     2641 tataacctct actttaacaa tcgttatatt aaaaatatgc cgattgttgc gaatttatca
     2701 catggaccag ttggctatat aggaccacga ggtttagtat tggagcaact ccaattgatg
     2761 gttaatcaat tagctttttt ccattcttac catgatgttc aatttattac tattgttcca
     2821 gaggaagaga tggataagtg gtcttggatg cgttggttgc cacatgaaac cttacaagat
     2881 gtcaatgttc gtggctttgt ttataatcaa agaagccgtg atcaagtgct caacagtctt
     2941 aaccaaattt taaaattacg tcgcacacaa cgcgaagata aatctgccaa agaagggact
     3001 ttattttctc cgcattatgt cgttattgta actgatgaaa agttaatttt agatcacgtc
     3061 atcatggaat tctttacgga agatcctaca gaattgggat gttctctcat ctttgttcaa
     3121 gatgttatga gttcactttc agaaaatatc aaaaccatta ttaatattaa agaccgtaat
     3181 actggtcagt tagttataga agaaggagag cttaaagaaa cagattttga attagatcat
     3241 ttcttagagg attatgacaa ggaaaatatt tcacgtcgcc tagctccgtt aaatcaccta
     3301 caaaatctta aatcttcaat accagaagca gttactttta tggaaatgta tcaagcagaa
     3361 gaatttgaag atttgcatgt tcaagagcgt tggataagcc atgctccgta taaaagctca
     3421 gctgttccac ttggcttacg tggtcaggat gatattgttt atctaaacct acatgaaaaa
     3481 gctcatggtc ctcatggatt agttgcagga acaacgggat cagggaagtc agaaattata
     3541 cagtcctata tcctttcttt agctgttaat ttccatccac acgatgtagc tttccttctc
     3601 atagactaca aaggtggggg gatggcaaac ctctttaagg acctgccaca cttacttggc
     3661 accatcacca acttagatgg tgcccaatcg atgcgtgctt tggtttctat taatgctgaa
     3721 ttgaaacgtc gtcaacgttt atttgctaaa gctgatgtta atcatattaa ccagtatcag
     3781 aagaaataca aacttggaga agtttcagaa ccaatgcctc atctcttctt aatctccgat
     3841 gagtttgccg aattgaaatc caaccaacct gagtttatga aagagcttgt ctcaacagcg
     3901 cgaattgggc gttccctagg gattcactta atcctagcga cccaaaaacc atctggtgtg
     3961 gtagatgacc aaatctggtc aaactcacgc ttcaaactag ctcttaaagt agcagaccgc
     4021 ggcgactcca tggaaatgct ccatactcca gatgccgccg agattacaca agcaggacgt
     4081 gcctacctcc aagttggtaa taatgaagtc tatgaactct tccaatcagc atggtcagga
     4141 gcagattacc aacctgaaaa agacgaccaa gggattgaag accataccat ttattccatc
     4201 aacgaccttg gccaatacga aatcctaaat gatgatcttt caggcttaga ccaagccgaa
     4261 aatattaaag aagtacctac ggaattagat gctatcgttg aaaacattca agctttaacc
     4321 aaagaaatgg gcatctcaga cttacctcaa ccgtggctac cgcctctaag caatcagatt
     4381 gcagtaaccg atctccgtaa ggagggatca gtagacctct ggagcaaggc cccatcttac
     4441 aaagctgttc ttggctttat ggatatacca agccaacaag cacaagaagt ggcttaccac
     4501 gattttgaag acgatgggca tttgtcaatt tttgcaggac caagtatggg gaaatccaca
     4561 gcccttcaaa ctgtaacaat ggacttagca cgtcataata gtccagaatt tttaaacctt
     4621 tacctctttg attttggtac caatggcctc ttacctctaa gacgtttgcc acatgtagca
     4681 gatttcttta ccattgatga tgacgaaaaa atagccaagt ttattgcccg tatcaaagtt
     4741 gaaatgtctg atcgtaaaaa agccctcagt cgctacaatg tcgcgacagc aaaactttac
     4801 cgtcaggtat ctggggaaac catgcctcaa atcctgattg ttattgacag ttatgaaggg
     4861 ctacgggaag ctcaaacacc gactaactta gaagcttgtt tccaaaatat ttcgcgtgat
     4921 gggtcgtcac ttggcatttc cttagttatc tcagctggtc gtacggcagc tttgaggtcg
     4981 tccttaatgg caaaccttaa agaacgtatc gctcttaaat taacagatga ctctgaatca
     5041 cggacacttg tcggtcgtca ccaacacatc atggaagata ttccaggacg tggattaatc
     5101 aaacgtgatg acattgaagt gttacaagtg gctctctcaa cagaaggcac tgaaaccttt
     5161 gacatcatca ataacatcca aaacgaatct gacgccatga acagcaaatg gactggacca
     5221 agaccaaaag caataccaat tgttcccgaa gaattaacct ttgacgactt tatggctaca
     5281 gatagtgttc aagctgacct atcagctaat cgtttaccac ttgggcttga aatggttgac
     5341 gttgaaagtt atagtttagc acttaatcgc tttaagcata tgctttacat gtctgattct
     5401 gacgagtcac tcgaagcagt tggttcccat atcatcaagg tcttgcttaa cgtcccaaat
     5461 taccataaca tgatcatcga ttcgcttggc gaatatgaaa gctatcaagg tcaggtacgt
     5521 acttatgtgg gttctgatat gattgaagat atggcggatc agttgatcta tgagttgcag
     5581 caacgtgaaa gccaatctga gatagctgaa tggtttatca tgattccaga ttttgaaagc
     5641 tttgtggcta agagcaatat gaagcttgaa caggttcagg cattgcttga caatggacca
     5701 cgctatggtt tacatctgat tcttggatca agctataact atgttgggac taagatcgat
     5761 gctgtgccta aatacgttcg cacaacgacc cagtatgtgc tggctggcat gaggctcatg
     5821 gatcaaacct tccttgaaaa agtgtataat agtaaggaaa tccgtccaaa caaagacgaa
     5881 gcctacattc acgatagaaa aacctatcaa aaattaaaac tcaccataga ctaagaaagg
     5941 agaccaaatg gcaaaaatac aaagtgatgc ctccgtcgca tccacctgtg caactgcaat
     6001 acaatcagga gccagtggaa tcacagcagt aggagctcca actaaggatt caaacagtga
     6061 ttatagtggt aatagcgatg cttcaactta catcgatgga gaggcaagta gtagtagcca
     6121 aattgctgaa aaaataaatg agttcatagc tctcattcat tctacggtga atgagtttga
     6181 agctattgac caaaaaatta gccaagagat tgcggcaaat actaaatata caatgcctag
     6241 taatgacagc tctgttatat caaatccgga ttttaagcct aaatatggtt tgttttaata
     6301 ggtgatgtta tgactgaacg aacattcgaa gatattgaac ttgacttaaa gttattccaa
     6361 ataaagcttg ataatgctga gaatagtaaa aggctcttac aaaaattgaa aaacgacgtc
     6421 atggagttac aaatagagtt actagaatca ttaaaactcg gtgatgccta tttaacagaa
     6481 tcagaagaat tagaagagaa taatgacttt attctaacgg taaatagtga aacactaagt
     6541 ttagaagaat cctatgacaa taggataaac ttagtttcta aagagattat ggattatgaa
     6601 aatgcattag ataagttgta ttatgaaaaa cagagtttga tgcaaaaaag taatgaaaga
     6661 aaaggaggtt aaaaatggta aaagttagtg taagttctgt aggaactcaa gcatcaacag
     6721 tagctatttc tatgtttagt cgtgtatcgg ctttaaatga tgcaataaca aaactatcat
     6781 cttttgcaga ggctgcaact cttcaaggga ctgcttattc aaatgcaaaa agctatgcta
     6841 ctggaacgtt aactccgatg cttcaaggaa tgattctttt ctctgaaaca ttgagtgaga
     6901 aatgtacaga attacaaacc ttatatgtct caatttgtgg tgatgaggat ttagactctg
     6961 tcgttttaga atcaaaatta gcaagtgata gggcatcatt aaagattgct gaagcacttt
     7021 tagagcatct taacgatgat ccagaacctt ccaaatctgc cataagttct acaaaaagta
     7081 atattaaaaa attaaaaaaa cgtataaaat ctaatcaaaa gaaattagac aaccttaatg
     7141 aatttaacgc ccattcagca acagtatttg cggacatttc taatgcacag tcaactgtta
     7201 accaagcact agcggctgtt tcaacaggat tttctggata taatagtaaa accggagctt
     7261 ttggaaaacc aacatccgga cagatggaat ggacaaagac agttaagaag aattggaaag
     7321 agcgagaaga cgccaaagct gaagaactga aaagtaaaaa ggctgaagaa agtaagaaag
     7381 cttcaaaaat tgaaaatact actaaaaaaa gtaatgtttc agttgataaa aagaaattaa
     7441 taaaagcggc taatgaagcg tataaattag gagaaattaa aaaagatacc tatgaatcaa
     7501 ttatcagtgg tttaagtaat gcatcggctg ccttacttaa agaggtagct aaatcaaaat
     7561 tgactgacac agctcggcta ttgatgtaaa tccttcttac gctaacttca ttagaggctc
     7621 gagccaattt gtaaaatatg gagcgcctgt acttggggct gcaattgact ttggagcagc
     7681 tcaagtaaaa ggtggagaag atgtagaaga tgcaactatg aagacaggag cacccgtaat
     7741 aattggtagt gctgcaaaag ttgtaggaac ggctgcgggt accgcaacag ctggactatt
     7801 aatttcaaca ggtgtacttg caccgcctgc tcctgttgtt ggttctgctg tggactttgg
     7861 tgctacagca gtaataggtg taggtggatc gatggtgttc gattatgttt atgataataa
     7921 agatgaaata aatcatatta taaaaagagc atcgaaggat acaattaaca atgttagtga
     7981 tttttttgaa ttaatgtatg attcaacagg tagtataaat tgagaaggaa tttattttga
     8041 tacatagaga gaaatcatat gcagaaatat atcatgacgg aaatcaaggt tattattata
     8101 attttgatac aaaaattttt tacattaaaa gttttgaaac gcctaattat caaaaagaaa
     8161 atttagaaaa tattaaaaaa tattctagta ttattacgtt tcttgttctc atactcattc
     8221 ctattaataa ttggtataga acaatagcta atttaaagtt gaatttaatt cttgcaataa
     8281 tgtttatcgt aattgcacta gtagtagtta acaaattaaa caatatagtt tcgaaacaaa
     8341 agagaaatat cgataatatt agttacgaga aaatggaatt tgacaaagaa aaaatagcaa
     8401 atataattgt ttctgctgaa gaattgtcga ataaactgtt atttttatta g
//
LOCUS       DATRMA010000077         8206 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.077, whole genome shotgun sequence.
ACCESSION   DATRMA010000077 DATRMA010000000
VERSION     DATRMA010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8206
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.077"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            complement(<1..993)
                     /locus_tag="VBC28_001691"
     CDS             complement(<1..993)
                     /locus_tag="VBC28_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000730412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEO6815655.1"
                     /translation="MKKKSLISNLILINSITLFLGFYLIYFSVNYVIKDYVHQMTRNS
                     MQANFTILDSIYNNKKIPDENMVNQDSIFVWSYYAIFDQHYRMKYGTDNHKKESHDII
                     RYLTQKRLWQISKEKDGMFVTIKKKTYYVMTKDYSGILVDGSIKKVPKAQSQLFHVIN
                     FSDITYTQHLITKINHFLIVILVLTYIPMLFIMRKTFTGIRESIQSVQTYISSLWKNQ
                     GNHQSSQKEIVFSDFDPLLLESQEMANRIYQAEESQRNFFQNASHELRTPLMSIQGYT
                     EGVQEGIIDAELAHSVILQESKKMKQLVDDIILLSKLDSNLSDQKDEFSLNELLN"
     gene            complement(990..1676)
                     /locus_tag="VBC28_001692"
     CDS             complement(990..1676)
                     /locus_tag="VBC28_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO6815656.1"
                     /translation="MTKIIYFADDEKNIRDLVVPFLEHDGFTVRAFETGDLLLEAYKN
                     QKPDLVILDIMMPGTNGLDVMKSIRQYDNIPIIMLTARDSDVDFITAFNLGTDDYFTK
                     PFSPIKLSLHVKALFKRLDEKAIKNDTQYQFLDLTLDTEKRIALLSNEEMPLTKTEFD
                     FLLVLIEKPETAFSRETLLNRIWGFDDIESRAVDDTIKRLRKKFKQYHSQVSIKTVWG
                     YGFKLIKEDK"
     gene            complement(1909..6621)
                     /locus_tag="VBC28_001693"
     CDS             complement(1909..6621)
                     /locus_tag="VBC28_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEO6815657.1"
                     /translation="MNTKQRFSIRKYKLGAVSVLLGTLFFLGGITNVAADSVINKPSD
                     IAVEQQVKDSPTSIANETPTNNTSSALASTAQDNLVTKANNSPTETQPVAESHSQATE
                     TFSPVANQPVESTQEVSKTPLTKQNLAVKSTPAISKETPQNIDSNKIITVPKVWNTGY
                     KGEGTVVAIIDSGLDINHDALQLNDSTKAKYQNEQQMNAAKAKAGINYGKWYNNKVIF
                     GHNYVDVNTELKEVKSTSHGMHVTSIATANPSKKDTNELIYGVAPEAQVMFMRVFSDE
                     KRGTGPALYVKAIEDAVKLGADSINLSLGGANGSLVNADDRLIKALEMARLAGVSVVI
                     AAGNDGTFGSGASKPSALYPDYGLVGSPSTAREAISVASYNNTTLVNKVFNIIGLENN
                     RNLNNGLAAYADPKVSDKTFEVGKQYDYVFVGKGNDNDYKDKTLNGKIALIERGDITF
                     TKKVVNAINHGAVGAIIFNNKAGEANLTMSLDPEASAIPAIFTQKEFGDVLAKNNYKI
                     VFNNIKNKQANPNAGVLSDFSSWGLTADGQLKPDLSAPGGSIYAAINDNEYDMMSGTS
                     MASPHVAGATALVKQYLLKEHPELKKGDIERTVKYLLMSTAKAHLNKDTGAYTSPRQQ
                     GAGIIDVAAAVQTGLYLTGGENNYGSVTLGNIKDKISFDVTVHNINKVAKDLHYTTYL
                     NTDQVKDGFVTLAPQQLGTFTGKTIRIEPGQTQTITIDIDVSKYHDMLKKVMPNGYFL
                     EGYVRFTDPVDGGEVLSIPYVGFKGEFQNLEVLEKSIYKLVANKEKGFYFQPKQTNEV
                     PGSEDYTALMTTSSEPIYSTDGTSPIQLKALGSYKSIDGKWILQLDQKGQPHLAISPN
                     DDQNQDAVAVKGVFLRNFNNLRAKVYRADDVNLQKPLWVSAPQAGDKNYYSGNTENPK
                     STFLYDTEWKGTTTDGIPLEDGKYKYVLTYYSDVPGSKPQQMVFDITLDRQAPTLTTA
                     TYDKDRRIFKARPAVEHGESGIFREQVFYLKKDKDGHYNSVLRQQGEDGILVEDNKVF
                     IKQEKDGSFILPKEVNDFSHVYYTVEDYAGNLVSAKLEDLINIGNKNGLVNVKVFSPE
                     LNSNVDIDFSYSVKDDKGNIIKKQHHGKDLNLLKLPFGTYTFDLFLYDEERANLISPK
                     SVTVTISEKDSLKDVLFKVNLLKKAALLVEFDKLLPKGATVQLVTKTNTVVDLPKATY
                     SPTDYGKNIPVGDYRLNVTLPSGYSTLENLDDLLVSVKEDQVNLTKLTLINKAPLINA
                     LAEQTDIITQPVFYNAGTHLKNNYLANLEKAQTLIKNRVEQTSIDNAIAALRESRQAL
                     NGKETDTSLLAKAILAETEIKGNYQFVNASPLSQSTYINQVQLAKNLLQKPNVTQSEV
                     DKALENLDIAKNQLNGHETDYSGLHHMIIKANVLKQTSSKYQNASQFAKENYNNLIKK
                     AELLLSNRQATQAQVEELLNQIKATEQELDGRDRVSSAENYSQSLNDNDSLNTTPINP
                     PNQPQALIFKKGMTKESEVAQKRVLGVTSQTDNQKVKTNKLPKTGESTPKITYTILLF
                     SLSMLGLATIKLKSIKRE"
     gene            6944..7636
                     /locus_tag="VBC28_001694"
     CDS             6944..7636
                     /locus_tag="VBC28_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEO6815658.1"
                     /translation="MEKGFKEGVKDALPTALGYISIGLAFGIVASASDLSAIEVGLMS
                     ALVYGGSAQFAMCALLLAKADLMTITMTVFLVNLRNMLMSLHATTIFKSAHLMNQLAI
                     GTLITDESYGVLLGEALHHKVVSPSWMHGNNVMSYLTWVISTIIGTLLGSTIPNPEMF
                     GLDFALVAMFIGLFVFQLFGMLSDGKRLVVYVLASVGLSYFLLATFLSGALSVLLATV
                     VGCSVGVVLDDK"
     gene            7626..7949
                     /locus_tag="VBC28_001695"
     CDS             7626..7949
                     /locus_tag="VBC28_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEO6815659.1"
                     /translation="MINSTVFLAILLGFVVSWVPRVAPFIFVKYKGLPNSLVKFLKFL
                     PLSILFALTFSSFFSVKLGQLPTLKWLDLLAGIPTLVVTIRYRNLFYTVLTGIVTMAL
                     LRLLF"
BASE COUNT         2363 a         1365 c         1494 g         2984 t
ORIGIN      
        1 attaagtaat tcatttaaag aaaattcatc tttttgatca cttaagttac tatctaattt
       61 agagagtaag ataatatcgt ctacgagctg tttcattttt ttgctctctt gcagaataac
      121 agagtgtgcg agctcggcat caataatacc ctcttgaact ccctcagtat atccttggat
      181 ggacattaaa ggtgtccgta actcgtggga ggcattttga aagaagtttc tttggctctc
      241 ttctgcttgg tagattcgat ttgccatttc ttggctctca agtagaagag gatcgaaatc
      301 agaaaagaca atttcttttt gactagattg atgattgcct tgatttttcc aaaggctgga
      361 aatataagtt tgtactgatt gaatagattc tctaattcca gtgaaagtct tcctcataat
      421 aaatagcatg ggaatatagg ttagtactaa gataactata agaaaatgat taattttagt
      481 aattagatgc tgggtgtaag taatgtctga aaagttaatc acatgaaaaa gttgcgactg
      541 tgctttagga acttttttta tgctaccatc aacaaggata ccagagtagt ctttggtcat
      601 tacataatag gtttttttct tgatggttac aaacatacca tctttttcct tagaaatttg
      661 ccacaatctt ttttgggtaa gatagcgaat aatatcatgg ctctcttttt tatgattatc
      721 agtgccatat ttcatacgat aatgctggtc aaaaatagca tagtaagacc aaacaaagat
      781 gctatcttgg ttaaccatat tttcatctgg tattttcttg ttgttataga tactatctaa
      841 aatggtgaaa ttcgcctgca tggaattcct tgtcatttga tggacatagt ctttaatgac
      901 atagttgaca gaaaaataga tgaggtaaaa accaagaaat agagtgatgc tattaataag
      961 gataagattg ctaattaaac ttttcttttt catttgtcct ctttgattaa tttgaagccg
     1021 taaccccaga cagttttgat actaacctga ctgtggtatt gcttaaattt tttacgtaat
     1081 cgtttaatag tgtcatcaac tgctctgctt tcaatatcat caaatcccca aatacgatta
     1141 agcagtgttt ccctagaaaa agcagtttca ggtttttcaa ttaatactaa aagaaaatca
     1201 aattctgtct tggttaaggg catttcttca tttgataata gggctattcg cttttcggta
     1261 tccagagtta aatctaggaa ctgatactga gtgtcatttt tgattgcttt ttcatcaagg
     1321 cgtttgaaca atgctttaac atgtagacta agttttatag gtgaaaaagg cttggtgaag
     1381 taatcgtcag ttcctaaatt aaaagccgta atgaaatcaa catcagaatc tctagctgtt
     1441 aacatgataa taggaatatt atcatattgg cggatgcttt tcataacatc taaaccatta
     1501 gttccaggca tcattatgtc taatataact aaatcgggtt tttggttttt ataggcttct
     1561 aagagaaggt ctccagtttc aaatgctcga acagtgaaac catcatgttc taaaaaagga
     1621 accaccaagt cacgaatatt cttttcatca tcagcgaaat aaattatttt tgtcatgtat
     1681 acctcaatac ccatagttat aatttgactt ttagctagct gtttatgtca tatcaaatat
     1741 aacaaaaaaa gagttttttt cccattcttt gaagtgttat ggtgttataa accaagatac
     1801 tgcagagaaa aggtctttag gaacatcatt ttaagctcct caaaaaggca ccagtcacta
     1861 actattaata gctagtaatt gatgccttgc tctatttttt aaagtatttt attctctttt
     1921 gatagacttt agtttgattg ttgccagacc tagcatagag agactaaata gcaatattgt
     1981 ataggttatt ttaggagtgc tttcgcctgt tttaggaagc ttgtttgtct ttaccttttg
     2041 attatcggtt tggctagtca cccctaagac acgcttctga gcaacctcac tttctttagt
     2101 catgcctttt ttgaatatca acgcctgggg ctgatttggc ggattgatag gtgtggtatt
     2161 gagagagtca ttatcattga gtgattgact ataattctct gcggaagaaa ctctatcacg
     2221 gccatcaagc tcttgttcgg ttgcttttat ttggtttaat aactcttcaa cttgagcttg
     2281 tgtagcttgt ctattggaaa gcagcaattc tgctttcttg ataaggttat tataattttc
     2341 tttagcaaat tgactggcgt tctgatattt agatgatgtt tgtttcagaa cgtttgcttt
     2401 aattatcata tggtgtaaac cagagtaatc agtttcatga ccatttaatt gatttttagc
     2461 aatatcaaga ttttctaagg ctttgtctac ttctgattga gtgacgttag gtttttgtag
     2521 aaggtttttc gccaattgga cttgattgat ataagttgat tggcttaatg gactagcatt
     2581 aacaaattga tagtttccct tgatttctgt ttcagctaaa atagcttttg ccagtaaaga
     2641 agtatctgtt tctttaccgt taagagcttg gcgactttct ctcaaagcag caatagcatt
     2701 atcaatactt gtttgttcca ctctattttt aattaaagtt tgtgcctttt caagattagc
     2761 taggtaatta ttttttaagt gagttccagc attataaaac acaggctggg taataatatc
     2821 agtttgttct gctagggcat taatcagagg agctttatta atcagcgtca attttgttag
     2881 gtttacctga tcttctttta cggatacaag taaatcatct aagttctcta aagtgctata
     2941 cccactaggc agcgttacgt ttaaacgata gtctcctaca ggtatgtttt taccatagtc
     3001 agtaggagaa taagttgctt ttggtagatc aacaacagta tttgtcttag taaccaactg
     3061 gactgttgct ccttttggta aaagcttgtc aaattcaacg agtaaggctg ctttcttgag
     3121 taagttaact ttaaataaga cgtctttaag gctatctttt tcagaaatag ttacagtgac
     3181 acttttggga ctgattagat ttgctcgttc ctcatcgtat aagaatagat caaacgtata
     3241 ggtaccaaaa ggcaatttca ataaattgag gtctttcccg tgatgttgct ttttgatgat
     3301 attacctttg tcatctttga cagagtaaga gaaatcaata tcgacattac tgttaagctc
     3361 agggctaaac actttgacgt ttactaaacc atttttattg ccaatattga tcaaatcttc
     3421 gagttttgct gatactagat tgcctgcata atcttcaaca gtatagtaga catgagagaa
     3481 atcgttaacc tctttaggta gaataaagct accatccttt tcttgtttga taaatacttt
     3541 gttatcttca acaaggatac cgtcttctcc ttgttgacgt aagacgctat tataatgacc
     3601 atctttatct ttttttaagt aaaaaacttg ttctctaaag ataccagatt ccccgtgttc
     3661 tactgcagga cgagctttga agatacgtct atctttgtca taagttgctg ttgttagtgt
     3721 aggagcttgt ctatccaaag tgatatcaaa caccatttgt tgtggcttag agccagggac
     3781 atcagagtaa taagttaaaa cgtatttgta ttttccatct tctaaaggaa taccatcagt
     3841 agtggttcct ttccattctg tgtcatataa aaatgtagat tttggatttt cagtatttcc
     3901 gctgtagtaa tttttatctc ctgcttgggg agcacttacc catagtggtt tttgtaaatt
     3961 aacatcatct gcacgataga ctttggctct taaattattg aaattacgta agaaaacacc
     4021 tttcactgca acggcatctt gattttggtc atcattaggt gaaatggcta gatgaggctg
     4081 gcctttttga tctagttgta agatccattt tccatctata gacttatagc ttcccaaggc
     4141 tttcaattgg ataggactag taccgtctgt tgagtagata ggctctgaac ttgtagtcat
     4201 taaggcagta taatcttctg aaccaggaac ttcgtttgtt tgttttggtt ggaaataaaa
     4261 tcccttttct ttgttagcaa caagcttata aatggatttt tctaaaactt ctaagttttg
     4321 gaattctccc ttaaatccaa cataaggaat actaagaact tccccaccat caacagggtc
     4381 tgtaaaacgt acgtagcctt ctaggaaata gccgtttggc attacttttt ttaacatgtc
     4441 atggtatttc gaaacatcta tatcaattgt aatcgtttgg gtttgccctg gttcaatccg
     4501 tatcgttttc cctgtaaatg taccaagttg ttgaggagcc aatgtgacaa agccatcttt
     4561 aacttgatca gtatttaaat aggttgtata gtgtaaatct tttgcaactt tattgatatt
     4621 atgaacagta acatcaaagg aaattttatc tttaatattt cctaatgtaa cgctgccata
     4681 gttgttttcc ccaccagtta ggtataatcc tgtctgaact gctgctgcga catcgataat
     4741 acctgctcct tgttggcgtg gtgaggtgta agcgcctgta tctttgttta ggtgtgcttt
     4801 agcagtactc ataagaaggt atttgacagt tctttcaatg tcaccttttt taagttctgg
     4861 atgttctttc aataagtatt gtttaactag agcagtagca ccagcgacat ggggagaagc
     4921 catacttgtc ccactcatca tatcatattc attatcattg atagcggcgt aaatagagcc
     4981 tccaggagca gataagtcag gttttaattg tccgtctgct gttaaccccc agcttgaaaa
     5041 gtcagatagg acacctgcat tagggttggc ttgtttattt ttgatattgt taaatacaat
     5101 tttatagttg tttttagcta aaacatctcc aaactctttt tgggtaaaaa tagcaggaat
     5161 agcacttgct tcaggatcta aactcattgt tagattagct tccccagctt tgttattaaa
     5221 gataatagct cccacagcac cgtgattaat agcattgacg actttttttg taaaagtaat
     5281 atctccacgt tcaattaagg cgattttacc atttaaagtt ttgtccttat aatcattgtc
     5341 gtttcctttt cctacgaaaa cataatcata ttgtttccct acttcaaagg tcttatcact
     5401 aactttagga tctgcataag cagctaatcc gttgttgaga tttctgttgt tttctaatcc
     5461 gataatgttg aagactttat taaccagtgt tgtattatta tatgatgcta cagaaatggc
     5521 ctcacgagct gttgatggac taccaactaa accataatca ggataaagag cagaaggctt
     5581 tgatgctcca ctcccaaatg taccgtcgtt acctgctgct ataacaacag aaacgccagc
     5641 gagtctagcc atctctaaag cttttataag tcggtcatcg gcattaacta aagacccatt
     5701 agctccacct aaacttaaat taatgctgtc agcaccgagt ttaacggcat cttcaatagc
     5761 tttaacataa agggctggtc cagttcctct tttttcatca gagaagactc tcataaacat
     5821 tacttgtgct tcaggagcaa caccatagat taattcattt gtatctttct tgctaggatt
     5881 agctgttgcg atactggtta cgtgcatacc atgagaagtg cttttcacct cttttagctc
     5941 tgtattgaca tcaacatagt tgtgaccaaa gattactttg ttgttatacc attttccata
     6001 gtttatacca gcttttgctt tagcagcatt catttgctgt tcgttttggt attttgcttt
     6061 tgtcgaatca tttaattgga gagcatcgtg attgatatct agtcctgagt caataattgc
     6121 tacaacagtt ccctctcctt tgtagcctgt gttccatact ttggggacag tgataatttt
     6181 attactatca atgttttgag gggtttcttt agaaatagct ggtgtagatt tgactgctaa
     6241 attttgtttg gttaaaggag ttttagaaac ttcttgagtg ctttcaaccg gttgatttgc
     6301 gactggggaa aatgtctcgg tggcttgaga gtgagactca gctactggtt gtgtttctgt
     6361 tggactatta ttagcctttg taacaagatt gtcttgagct gtcgacgcaa gggctgatga
     6421 cgtgttgtta gtaggtgtct catttgctat gctcgttgga ctgtctttta cttgctgttc
     6481 aactgcaata tcagatggct tatttatgac agaatcagca gctacatttg tgataccacc
     6541 taaaaaaaat agggttccca aaagtacaga tacggcacct aacttatatt tccggattga
     6601 aaaacgctgt tttgtgttca taaaaatctc cttttcttgt aacataataa ttacaaaaac
     6661 tattaaaaca taaaaaatca aaattttcta caattttctg aattttcatt acaaattatt
     6721 ttgtggtaaa tttaagaccg tacctaaagc attttttaaa aatcattcta tgaaaataat
     6781 aatattgtcc aactatatgg ctacaatatc aaaggtttat agaataatta caggatattt
     6841 attagtctaa ttgctggcaa agataaataa tcattcctct tctttcttag taatcaccgt
     6901 tgtgctataa taactgaata tactgaaaat ttagggggat atgatggaaa aaggatttaa
     6961 agagggagtg aaagatgcct tgcccacagc tttagggtat atctcaatag gcttggcgtt
     7021 tgggatagtt gcttcagcat cagatttatc tgcaatagaa gttggtctta tgagtgcctt
     7081 ggtatatggg ggatctgctc agtttgctat gtgtgcgcta ttattagcaa aagcagactt
     7141 gatgactatt acgatgacag tatttttagt taatttgaga aatatgttaa tgagtttaca
     7201 cgcgactact atcttcaagt ctgctcacct aatgaatcaa ttagccatag gaactctaat
     7261 aacagatgag agctacggcg ttttacttgg agaagcatta catcacaaag ttgtatcgcc
     7321 tagttggatg catggtaata atgtgatgag ttatttgaca tgggtaatct caacgataat
     7381 aggaacttta ctggggagta ctattcctaa tccagaaatg tttggtttag actttgcatt
     7441 agtcgccatg tttataggtc tctttgtatt tcagttgttt gggatgttat ctgatggaaa
     7501 gcgacttgtt gtatatgttc ttgcttctgt aggtttaagt tacttcttat tagctacttt
     7561 tttgtcagga gccttatcag tattgcttgc gactgttgtt ggatgtagtg tgggagttgt
     7621 tttagatgat aaatagtacc gtttttcttg ctattctttt aggttttgtt gtttcttggg
     7681 tgccaagagt cgctccattc atatttgtaa aatacaaagg gctaccaaat agtcttgtga
     7741 aatttttaaa atttcttcct ctttctattt tatttgccct aacattctct agcttttttt
     7801 cagttaaatt aggacaattg ccaacactaa aatggttaga tttattggct ggcataccaa
     7861 ctctagttgt cactattcga tatcgaaatt tattttacac tgttcttact ggtatcgtta
     7921 ctatggcttt attgagatta ctattttaac gtgtaggaat ggaagattaa taaaaacaca
     7981 aaaaacgcat ctatttctag atacgttttt tgtgtttttg tctagacagg aacttttgat
     8041 agccgatgtg taatagtttg actaattgtt aataatatca acagattttt ctgtatcttg
     8101 ataatatgag ttgattgtaa gattgctttt taaagatatc tacatgctgc tgttttaaaa
     8161 tttataaaaa ggtccatagt ggtttatata gtttaaaact atataa
//
LOCUS       DATRMA010000078         7907 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.078, whole genome shotgun sequence.
ACCESSION   DATRMA010000078 DATRMA010000000
VERSION     DATRMA010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7907
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.078"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            <1..395
                     /locus_tag="VBC28_001696"
     CDS             <1..395
                     /locus_tag="VBC28_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000730412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEO6815660.1"
                     /translation="IIAYFKPLANKQKISITYRPDKHEKLLKGNEELIQRAINNILSN
                     ALRYAVSHIEISYTNQKLTISNDGPAISKEDLPYIFDRFYKGHGGQTGIGLAMTKEII
                     KQHHGNIIAESDSTSTTFTIYFNCHKIA"
     gene            488..1096
                     /locus_tag="VBC28_001697"
     CDS             488..1096
                     /locus_tag="VBC28_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000903267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome assembly protein"
                     /protein_id="HEO6815661.1"
                     /translation="MLHSKKIHSLSLIAVLSLATYTSLQPNHVTAEQSQKTSTVHMSQ
                     KTIEHKLKVADKEAAPLYAKIDHIQRHIEVKKAKDLKVIELYINKDINQLEKQNKRLL
                     TKFYTSIDNQTWDSTSEVKKLIDKTNLSTNEKDRLKLYFEQRAYLETRLNDRYQKFDN
                     SIENQNKELKILTSKIEKIYQKHGITKEVLKTYYAKKTVRAD"
     gene            complement(1183..3684)
                     /locus_tag="VBC28_001698"
     CDS             complement(1183..3684)
                     /locus_tag="VBC28_001698"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEO6815662.1"
                     /translation="MTFYNHKEIEPKWQAFWADNHTFKTGTDASKPKFYALDMFPYPS
                     GAGLHVGHPEGYTATDILSRFKRAQGHNVLHPMGWDAFGLPAEQYAMDTGNDPAEFTA
                     ENIANFKRQINALGFSYDWDREVNTTDPNYYKWTQWIFTKLYEKGLAYEAEVPVNWVE
                     ELGTAIANEEVLPDGTSERGGYPVVRKPMRQWMLKITAYAERLLEDLEEVDWPESIKD
                     MQRNWIGKSTGANVTFKVKDTDKDFTVFTTRPDTLFGATYAVLAPEHALVDAITTADQ
                     AEAVAEYKRQASLKSDLARTDLAKEKTGVWTGAYAINPVNGKEIPVWIADYVLASYGT
                     GAIMAVPAHDERDWEFAKQFNLDIIPVLEGGNVEEAAFTEDGLHINSDFLDGLDKAAA
                     IAKMVEWLEAEGVGNEKVTYRLRDWLFSRQRYWGEPIPIIHWEDGTSTAVPESELPLV
                     LPVTKDIRPSGTGESPLANLTDWLEVTREDGVKGRRETNTMPQWAGSSWYYLRYIDPH
                     NTEKLADEELLKQWLPVDIYVGGAEHAVLHLLYARFWHKVLYDLGVVPTKEPFQKLFN
                     QGMILGTSYRDSRGALVATDKVEKRDGSFFHVETGEELEQAPAKMSKSLKNVVNPDDV
                     VEQYGADTLRVYEMFMGPLDASIAWSEEGLEGSRKFLDRVYRLITTKEITEENSGALD
                     KVYNETVKAVTEQVDQMKFNTAIAQLMVFVNAANKEDKLFSDYAKGFVQLIAPFAPHL
                     GEELWQVLTASGQSISYVPWPSYDESKLVENEIEIVVQIKGKVKAKLVVAKDLSREEL
                     QDLALANEKVQAEIAGKDIIKVIAVPNKLVNIVVK"
     gene            4061..5308
                     /locus_tag="VBC28_001699"
     CDS             4061..5308
                     /locus_tag="VBC28_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO6815663.1"
                     /translation="MSPLNINKNNWRALIAAIVASGTDDLNIMFLAFSMSTIITDLHL
                     SAAQAGWIGTITNLGMLVGGLIFGLLADRYNKFKVFKWTILIFSIATGLVFFTTNLSY
                     LYIMRFIAGIGVGGEYGIAIAIMAGIVPTNKMGRISSLNGIAGQVGSISSALLAGWLA
                     PALGWRGLFLFGLLPIVLVLWMQFAVDDKDILDQYNTDADDEPLDISIKALFDTPVLA
                     TQSLALMVMTTVQIAGYFGMMNWLPTIIQTNLNVSVKNSSLWMIATILGMCLGMLVFG
                     QLLDKFGPRLVYGCFLLSSAICVYLFQFATTMPSMIIGGAVVGFFVNGMFAGYGAMIT
                     RLYPHHIRSTANNLILNVGRAIGGFSSVIIGMILDVSNVSMVMLFLASLYIVSFLSML
                     SIKQLKRQKYHTNLTQLDVKPTD"
     gene            complement(5351..6196)
                     /locus_tag="VBC28_001700"
                     /pseudo
     CDS             complement(5351..6196)
                     /locus_tag="VBC28_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000953624.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
     gene            6401..7597
                     /locus_tag="VBC28_001701"
     CDS             6401..7597
                     /locus_tag="VBC28_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 8"
                     /protein_id="HEO6815664.1"
                     /translation="MTYQKTVVLAGDYSYIRQIETTLKSLCVYHENLSIFIFNQDIPQ
                     EWFLAMKDRVGQTGNQIQDVKLFHDHLSPKWENKKLNHINYMTYARYFIPQYISADTV
                     LYLDSDLVVTTNLDNLFQISLDNAYLAAVPALFGLGYGFNAGVMVINNQRWRQENMTI
                     KLIEKNQKEIENANEGDQTILNRMFENQVIYLDDTYNFQIGFDMGAAIDGHKFIFDIP
                     ITPLPKIIHYISGIKPWQTLSNMRLREVWWHYNLLEWSSIISSKKVFGLDHPIKTQNY
                     RLNFLIVTTSDCIPSISELVTALPDCLFHIAAPTVMSDILISLLAFENIRLHKTYTPP
                     IIDKLLAEADIYLDINVFGKMEGILQKMSQSEKPILTTRECNSQLVPETVFASIDDLI
                     NYLQSI"
     gene            7662..>7907
                     /locus_tag="VBC28_001702"
     CDS             7662..>7907
                     /locus_tag="VBC28_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000409543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 8 glycosyl transferase"
                     /protein_id="HEO6815665.1"
                     /translation="MEKAVALAVDSNYLDKALVTIKSICVYNRNITFYLFNQDTPVEW
                     VRNINRKLEPLGSKLINVKIYNYDIAHLTTFLTVSTWF"
BASE COUNT         2469 a         1575 c         1377 g         2486 t
ORIGIN      
        1 gtattattgc ttatttcaaa cccctagcta ataaacaaaa gattagtatt acttatagac
       61 cagacaagca tgaaaagtta ctaaaaggta acgaagagtt gattcaacga gctatcaata
      121 atatccttag taacgcacta cgctatgctg tttctcatat tgaaatctct tacacaaatc
      181 aaaaactaac tatctcaaac gacggaccag ccatttcaaa agaagacttg ccttatattt
      241 tcgatcgctt ctataaagga catggcggcc aaacaggtat tggacttgca atgactaaag
      301 aaattatcaa gcaacatcat ggtaatatca tcgctgagag tgattcgacc tcaacaactt
      361 ttacgattta tttcaattgc cacaaaattg cctaactttt cctacaaaaa ttcaaagaag
      421 ttattgcttt tttaacctat aatacctata gatcaaaaag ttattctaaa agaaagtgag
      481 tttttatatg ttacattcta aaaaaataca ttccttatcg cttattgccg ttctctcttt
      541 agcaacatat acgagtttac aaccaaatca tgtaacggct gaacaatcac aaaaaacatc
      601 aactgttcat atgagtcaaa aaactattga acataagtta aaagttgcag ataaagaagc
      661 tgctcctctc tacgctaaaa tcgaccatat ccaacgacat attgaagtca aaaaagcaaa
      721 agatttaaaa gttattgaat tgtatattaa caaagatatc aaccaactag agaagcaaaa
      781 taaacgtcta ctaactaaat tctatacttc tattgataat caaacatggg atagcacaag
      841 tgaagtcaaa aaattgattg ataagacaaa cctatccact aacgaaaaag atagattaaa
      901 attatatttt gaacaacgtg cttaccttga gacaaggttg aacgaccgct atcaaaaatt
      961 tgataactct attgaaaacc aaaataaaga actaaaaata ttaacgtcaa aaatagaaaa
     1021 aatctatcaa aaacatggta ttacaaaaga ggtattaaaa acttactatg ctaaaaaaac
     1081 agtacgagct gactgattta gctaaaaata gaaaaatccc aaaagttaaa cttttgggat
     1141 tttatacgcg taaatatcac tatttctagt aaaatcttta tcttatttca ccacaatatt
     1201 aacgagtttg tttggtactg cgatgacttt gatgatgtct ttgcctgcga tttcggcttg
     1261 gactttttcg ttagcaagag caagatcttg caattcttcg cgtgaaaggt ctttagcaac
     1321 gacgagttta gctttgacct tacctttgat ttggacaacg atttcgattt cgttttcaac
     1381 caatttgctt tcgtcatagc ttggccatgg cacgtaagag attgactgtc ctgaggctgt
     1441 caagacttgc cagagttctt cacctaagtg cggtgcgaat ggtgcaatca attgaacaaa
     1501 gcctttggca tagtcgctaa agagtttgtc ttccttgttg gctgcattga caaagaccat
     1561 gagttgagcg atagctgtgt tgaatttcat ttggtcaact tgctcagtaa ctgccttaac
     1621 agtttcgttg taaaccttgt caagagctcc gctattttcc tcagtgatct ctttagtcgt
     1681 gatcaaacgg taaacacggt ccaagaactt acgtgagcct tcaagacctt cttcagacca
     1741 agcgattgaa gcatcgagtg gtcccatgaa catctcgtaa acacgaaggg tgtcagcacc
     1801 gtactgctca acaacatcat ctggattgac aacgttctta agtgatttag acatcttagc
     1861 aggtgcttgt tcaagctctt caccagtttc cacatggaag aagctgccat cacgtttttc
     1921 aaccttgtca gttgccacaa gagcaccacg gctgtcgcgg tagcttgttc ccaaaatcat
     1981 cccttggtta aagagttttt ggaatggctc tttggtaggt acgacaccga gatcataaag
     2041 aaccttgtgc cagaagcgag catagagaag gtgaagcacg gcatgctctg caccaccgac
     2101 atagatatca actggcaacc attgtttgag gagttcctcg tcagctagtt tttcagtgtt
     2161 gtgtggatcg atgtagcgga gatagtacca gcttgaacct gcccattgtg gcatggtgtt
     2221 ggtctcacga cgacctttaa cgccatcttc acgagtgact tccaaccagt ctgtcaagtt
     2281 agcaagtggg ctctcaccag tacctgaagg acggatatcc ttagtaactg gaagaactag
     2341 tggcaattcg ctctcaggaa ccgcagttga tgttccatct tcccagtgga tgattgggat
     2401 tggctcaccc cagtaacgct gacggctgaa gagccagtca cgaagacggt aagtaacttt
     2461 ctcattacca acaccttctg cttcaagcca ctcgaccatt ttggcaatag ctgccgcttt
     2521 gtcgagacca tctaggaagt ctgagttgat gtgaagaccg tcttctgtaa aggcagcctc
     2581 ttcaacattc ccaccttcaa gaactggaat gatgtctagg ttgaattgtt ttgcgaattc
     2641 ccagtcacgt tcatcgtgag ctggaactgc catgatagca cctgtaccgt agctagcaag
     2701 gacatagtcc gcaatccaaa ctggaatttc tttaccatta actgggttaa tggcataggc
     2761 acctgtccag acaccagttt tttctttggc aaggtctgta cgagcaaggt ctgatttgag
     2821 actcgcttga cgtttgtatt cggcaacggc ttctgcttgg tcagctgttg taatagcatc
     2881 aaccaaggca tgctcaggag caagaactgc gtaagtcgca ccaaaaaggg tgtcaggacg
     2941 tgttgtaaag acagtgaagt ccttatctgt atccttaacc ttgaaggtta cattagcacc
     3001 agttgattta ccaatccagt tgcgttgcat gtccttgatt gactctggcc agtcaacttc
     3061 ttccaagtct tccagcaagc gctctgcata ggcagtgatt ttgagcatcc attggcgcat
     3121 tggttttctg acaactgggt agccaccacg ttctgatgta ccatctggaa gaacctcttc
     3181 gttggcgata gctgttccca attcttcaac ccagttaact ggaacttcag cttcgtaagc
     3241 tagacctttt tcataaagct tggtaaagat ccactgtgtc cacttgtagt agttagggtc
     3301 agttgtgtta acttcacgat cccagtcgta tgagaaacca agagcattaa tctggcgttt
     3361 gaagttagcg atgttttcag ctgtaaactc agctgggtca ttaccagtat ccatagcgta
     3421 ctgctcagca ggaagtccaa aagcatccca acccattggg tggaggacat tgtgaccttg
     3481 ggcacgtttg aaacggctga gaatatccgt tgctgtatag ccttctgggt gaccaacgtg
     3541 gagaccagct ccagatgggt atgggaacat atctagggca taaaacttag gttttgatgc
     3601 gtctgttcct gttttaaagg tatgattatc tgcccaaaaa gcttgccatt tgggttcgat
     3661 ttctttgtga ttatagaatg tcatcgcact tctccatgtc aaaaaagtat agtcttctta
     3721 ttataacacg aaaaatagca ataaaaaagg tgaaaatacc ctcaacagca ggttctctcc
     3781 ccacttatcc ttgacaataa actaaactta ctctatacta tttccaaatg acataatgct
     3841 ttaaccgtgt cctgtgagac atataagcga ccaaaatagg attctataca gaaatcctac
     3901 tatttttgcg agcgtttata gaactcaagg gtagcagctt gagttttttc ttttacatta
     3961 taatgagttg aagcagctat gacatctctg ttttagaatt ttttctattg acagtattta
     4021 ataacggtgg tctgtgagcc atcattaaag gaggagactt atgtcaccat taaatatcaa
     4081 taagaataat tggcgcgccc taatcgctgc cattgtcgca tccggaaccg atgatttaaa
     4141 tatcatgttc cttgcatttt ctatgtcaac tatcattact gatcttcatt tatctgcggc
     4201 acaggctggt tggattggga caattaccaa cttgggaatg ctcgtgggag gactaatctt
     4261 tggtttatta gctgatcgct acaataaatt taaagttttc aaatggacga ttctaatctt
     4321 ttcaatcgca actggtttag ttttcttcac tactaattta tcatatctct atatcatgcg
     4381 cttcattgca ggaattggcg ttggtggcga atatggtatc gctattgcca ttatggcagg
     4441 aattgtccca actaataaaa tgggccggat ttcttcacta aatggtatcg ctggtcaagt
     4501 tggatctatt agctcagcac tcttagcagg atggctagct ccagcactcg gttggcgggg
     4561 actttttctc tttggccttc tccctatagt tctcgtccta tggatgcaat ttgctgttga
     4621 tgataaagat atcttagatc aatataatac agacgcagat gatgaacctc tagatatcag
     4681 tataaaagct ttatttgata ctcctgtatt agccacacag agcctagcac taatggttat
     4741 gacaactgtt cagattgccg gatactttgg catgatgaac tggttaccaa ctattatcca
     4801 aaccaactta aacgtttctg tcaagaattc atcattatgg atgattgcaa ccattcttgg
     4861 aatgtgcctt ggcatgctag tatttggtca attgttagat aaatttggac cgcgtttagt
     4921 atacgggtgt ttccttctat catctgcaat ttgtgtctat ctctttcaat tcgcaacaac
     4981 aatgccttct atgattatag gcggggcagt tgtcggattc tttgttaatg gtatgtttgc
     5041 aggctatggt gccatgatta cacgtctcta tccacatcat attcgttcaa cagctaacaa
     5101 tcttatctta aatgttggtc gtgcaatagg tggcttttca tctgttatca tcggaatgat
     5161 tctagacgtt tcaaatgtct ctatggtcat gcttttctta gcaagtctct atatcgttag
     5221 ttttttatca atgctaagca ttaagcaatt aaaacgtcaa aaatatcaca ctaatttaac
     5281 acaattagat gtcaaaccaa ctgactgata ttacaaaaca gtattcctca attgagaaat
     5341 actgttttct ttatttcatt agataactat tcagctcagg catcacactt ttagcatctt
     5401 tcataccaag ctgataaata ctatcaagtt tatccggatt cttctctaag cggccaataa
     5461 ccaagctctt actcggtcta attgcaaata gatcgcctgt tttttcaagg ctcatgacct
     5521 tttcaagact attattatac tgttggtacc gattcgaggc tgtctttaca aaattaggat
     5581 atttcctata cagagtttta tacaatcgtc cacttgaagg ctttttctga taattgagcg
     5641 gcctagtcat cacaacaatc aacttgtcaa atcctaaacc acgggcaaaa tcaacgggaa
     5701 tactatcaga taaaccacca tctaagtact ttttcccctg tcaatcaacc atctttgaga
     5761 ctactggtaa tgctgaacta gcacgtaaaa tttccatttg ttcaaaaaca ctatcaattt
     5821 taaaatattc aggtttacca gatgtcatct ctgtagcaac tacgtaaaaa tcaatacttg
     5881 attttttaaa tgcttcatcg tcaaatacat ccaatttcat aggaacttca taataggtga
     5941 aatctttatt aacaaaattc cctgttcgaa accatgacct tagactcata tatttagggt
     6001 gggataaata ctttttattg tatcgcaaag ccctctctcg ttgtctagat acaaaattaa
     6061 caccaaacaa tgcaccagca gagacagata cgataccatc tatttttatt cctgcatcta
     6121 gaaaagcatc taaaactcca gcagtataaa gacctctcat tccgccaccc tctaaaacta
     6181 aaccaacaga caacataggc aatcactttc taatattgat atttgattaa agtatagcaa
     6241 aatatagctg ttttttctga aaaagaatat gttgtactat ataaactcca taattaattt
     6301 aataataatt tctgaatcac actcacctat gatagaataa tactaatagt ttttaaattt
     6361 atatttacta tatctaacta atataaataa aggatttttt atgacttatc aaaaaacagt
     6421 tgttttggct ggtgattatt cctacattag acaaattgaa accacattaa aatctctctg
     6481 tgtctatcat gagaatctct caatttttat ttttaatcaa gatattcctc aagaatggtt
     6541 tttagctatg aaagataggg ttggacaaac tggaaatcaa attcaggatg taaagctctt
     6601 ccatgatcac ttatccccaa aatgggaaaa taaaaagctt aatcatatta attatatgac
     6661 ctatgctcgt tatttcatac ctcagtacat ctcagctgat acagttttat atcttgactc
     6721 tgacttagtt gttactacta atttagataa cctctttcaa atttcactag acaatgcata
     6781 tttagctgca gttccagctc tttttgggct tggatatggg tttaatgctg gagtaatggt
     6841 aattaacaac caacgttggc gacaagaaaa tatgactatt aaattaattg aaaaaaatca
     6901 aaaggaaatt gagaatgcca acgaagggga tcaaacaatt cttaatcgca tgtttgaaaa
     6961 tcaggtaatt tatttagatg atacctacaa ttttcaaatt ggttttgata tgggagctgc
     7021 tatcgatggg cataaattta tttttgacat cccaattacc ccactcccaa aaattattca
     7081 ctacatttcg ggaatcaaac cttggcaaac attatcaaat atgagactcc gtgaggtatg
     7141 gtggcactat aatttacttg aatggtcaag tatcatatct agtaaaaaag tatttggttt
     7201 agaccaccca attaaaacac aaaattatcg tctcaatttc cttattgtta caacttctga
     7261 ttgtatacca tctatctcag aattagtcac tgcccttcca gattgtctat ttcacattgc
     7321 agcaccaaca gttatgtctg acattcttat aagcctatta gcttttgaaa atatccgcct
     7381 tcataaaact tacactccac ctattattga taaattatta gcagaagctg atatttatct
     7441 tgatattaat gtttttggaa aaatggaggg cattctacag aaaatgtcac aatctgaaaa
     7501 accaattctt actacaagag aatgcaatag ccagcttgtt ccagaaacag tttttgcatc
     7561 tatcgatgat ttaattaatt atcttcagtc tatataaaaa aattggcatc cattcactat
     7621 atacccaaca ttattctaaa cacttaacta ggagaattta aatggaaaaa gcagttgcac
     7681 ttgcagttga ttcaaactac ttggataaag ccttagtaac aataaagtct atttgtgttt
     7741 ataatagaaa tataactttt tatttattca atcaagatac cccagttgaa tgggtacgta
     7801 atataaacag gaaactagag cctctaggat caaaactgat taatgttaaa atatataact
     7861 atgatattgc tcatctaacg acttttctaa ctgttagtac atggttt
//
LOCUS       DATRMA010000079         7588 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.079, whole genome shotgun sequence.
ACCESSION   DATRMA010000079 DATRMA010000000
VERSION     DATRMA010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7588
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.079"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            complement(34..2829)
                     /gene="ppc"
                     /locus_tag="VBC28_001703"
     CDS             complement(34..2829)
                     /gene="ppc"
                     /locus_tag="VBC28_001703"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000019267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEO6815666.1"
                     /translation="MSHPKLESSSNKEIITEEVGLLKQLLDEATQKLIGSESFDKIEK
                     IVSLSLTDDYTGLKETISALSNEEMVIVSRYFSILPLLINISEDVDLAYEINYKNNLN
                     QDYLGKLSTTIDVVAGHENAKDILEHVNVVPVLTAHPTQVQRKTVLELTSKIHDLLRK
                     YRDVKAGIVNQEKWYADLRRYIGIIMQTDTIREKKLKVKNEITNVMEYYNRSLIKAVT
                     KLTAEYKALAAKKGIHLENPKPLTMGMWIGGDRDGNPFVTAETLRLSAMVQSEVIINH
                     YIEQLNELYRNMSLSINLTEVSPELVTLANQSQDNSVYRENEPYRKAFNFIQDKLVQT
                     LLNLKVGSSPKEKFVSRQESSDIVGRYIKSHIAQVASDIQTEELPAYATAEEFKQDLL
                     LVKQSLVQYGQDSLVDGELACLIQAVDIFGFYLATIDMRQDSSINEACVAELLKSANI
                     VDDYSSLSEEEKCQLLLKELTEDPRTLSSTHAPKSELLQKELAIFQTARELKDQLGED
                     IINQHIISHTESVSDMFELAIMLKEVGLIDANQARIQIVPLFETIEDLDNSRDIMTQY
                     LHYELVKKWIATNNNYQEIMLGYSDSNKDGGYLSSGWTLYKAQNELTKIGEENGIKIT
                     FFHGRGGTVGRGGGPSYEAITSQPFGSIKDRIRLTEQGEIIENKYGNQDAAYYNLEML
                     ISASIDRMVTRMITNPNEIDNFRETMDGIVSESNAVYRNLVFDNPYFYDYFFEASPIK
                     EVSSLNIGSRPAARKTITEISGLRAIPWVFSWSQNRIMFPGWYGVGSAFKHFIEQDEA
                     NLAKLQTMYQKWPFFNSLLSNVDMVLSKSNMNIALQYAQLAGSKEVRDVFNIILNEWQ
                     LTKDMILAIEQHDNLLEENPMLHASLDYRLPYFNVLNYVQIELIKRLRSNQLDEDYEK
                     LIHITINGIATGLRNSG"
     gene            3038..4837
                     /gene="pepF"
                     /locus_tag="VBC28_001704"
     CDS             3038..4837
                     /gene="pepF"
                     /locus_tag="VBC28_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEO6815667.1"
                     /translation="MKLKKRSEFPENELWDLTALYKDRQDFLLAIEKALEDIKVFKKN
                     YEGKLNCVEDFTSALMEIEHIYIQMSHIDTYAFMPQTTDFSNEEFAQISQAGSDFATK
                     ANVLLSFFNTALANADIKILDSLENNPHFKATIRQAKIQKQHLLSPEVEKALTNLNEV
                     LNTPYDIYTKMRAGDFDMEDFEVDGKTYKNSFVTYENYFQNHENAEIREKSFRSFSKG
                     LRKHQNAAAAAYLAKVKSEKLIADMRGYDSVFDYLLSEQEVDRSMFDRQIDLIMDEFG
                     PVAQRFLKHIADVNGIEKMTFADWKLDIDNELNPEVSINDAYDLVMKSVAPLGKEYSQ
                     EVERYQKERWVDFAANANKDSGGYAADPYKVHPYVLMSWTGRMSDVYTLIHEIGHSGQ
                     FIFSDNHQSFFNTHMSTYYVEAPSTFNELLLSDYLENQFDTARQKRFALAHRLTDTYF
                     HNFITHLLEAAFQRKVYTLIEEGGTFGAEQLNAIMKEVLTQFWGDAIEIDDDAALTWM
                     RQAHYYMGLYSYTYSAGLVISTAGYLNLKNNPNGAKEWLAFLKSGGSRTPLETALLIS
                     ADISTDKPLRDTINFLSNTVDQIINYSNLIEQG"
     gene            complement(4896..5285)
                     /locus_tag="VBC28_001705"
     CDS             complement(4896..5285)
                     /locus_tag="VBC28_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HEO6815668.1"
                     /translation="MKKYIKLFLLTVFATTLVACGQPSTSNKTTTSSTLEVGKVELVV
                     KEDTNVLSEKVVYHKGDTVLDVLKANYKVKEKDGFITSIDGISQDETKGLYWMFKVNN
                     KLAPKAANQIKVKKNDKIEFYQEVYKK"
     gene            complement(5269..5793)
                     /locus_tag="VBC28_001706"
     CDS             complement(5269..5793)
                     /locus_tag="VBC28_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEO6815669.1"
                     /translation="MIKNSTLTKLTRVAILSALCVVLRYAFAPLPNIQPITAIFLITV
                     VLFDLKEGVATVTITMLVSSFLMGFGPWVFLQIISFTLILCLWKFLIYPLTKAVCFGK
                     ITEVVLQTFFAGGLGVVYGVIIDTCFAWLYHMPWWTYVLAGLSFNMAHALSTCLFYPL
                     LLPILRRFRNEKIH"
     gene            5949..6797
                     /locus_tag="VBC28_001707"
     CDS             5949..6797
                     /locus_tag="VBC28_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001254333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HEO6815670.1"
                     /translation="MRRRIKPIVVAVFFSLFGLLLIIGHLHSTNTLKKELVEAKKTIP
                     SVKASKVPQKSTSSKDKEFVLKPIIDVSGWQLPKEIDYDTLSKNISGVVIRVFGGSKI
                     SKTNNAAYTTGIDKSFKTHIKEFQKRNIPVAVYSYALGSSVKEMKEEAQIFYKNAAPY
                     KPTFYWIDVEEETMSNMNKGVQAFRKELKRLGAKNVGIYIGTYFMTEQGISVKGFDAV
                     WIPTYGSDSGYYEAAPQTELKYDLHQYTSQGYLPGFNQPLDLNQIAVNKDKKKTYEKL
                     FGKVKE"
     gene            complement(6830..7447)
                     /locus_tag="VBC28_001708"
     CDS             complement(6830..7447)
                     /locus_tag="VBC28_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000404311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEO6815671.1"
                     /translation="MEILIAGGSGFLGKQIIKAALTKGHKVAYLSRHEGKGDIFKDPR
                     LTYIRGDITEADKIHLEDRTFDILIDCIGAIKPNQLDELNVKATQKAVALCHKNQIPK
                     LVYISANSGYSAYIKSKRKAEQIIKASGLDYLFVRPGLMYGEERPLSIFQAKCIKLFS
                     HLPFLGIVVQKVFPTKVVIVAEAIVTTLRKKPTQKILSIEELNNK"
BASE COUNT         2480 a         1387 c         1213 g         2508 t
ORIGIN      
        1 gggagaatag caatgatcct ccctctatca atattaacct gaatttcgta aacctgttgc
       61 aataccatta atagtaatat gaatcaactt ctcatagtct tcatctagtt ggttactacg
      121 aagacgttta atcaattcaa tttgaacata attcaaaaca ttgaaatatg gcaaacgata
      181 atctaaactt gcatgtaaca ttgggttttc ttctaataaa ttatcatgct gttcaatagc
      241 taaaatcata tctttagtta actgccattc attaaggatg atattaaaaa catctcgaac
      301 ttctttactt ccagctaact gagcatactg aagcgcaata ttcatatttg attttgataa
      361 aaccatatct acattagata aaagagaatt aaagaatggc catttttgat acatggtttg
      421 taattttgct aagtttgcct catcttgttc aataaagtgc ttgaaggctg atccaacacc
      481 ataccatcct ggaaacataa tacgattttg tgaccatgaa aatacccatg gaattgcacg
      541 taaacctgaa atttctgtaa ttgttttacg tgctgctggc cgagaaccaa tatttaaact
      601 tgatacctct ttaattggac ttgcttcaaa gaaatagtca tagaaataag gattatcaaa
      661 aactaaattt ctataaacgg cattactttc agacacaata ccatccattg tctcacggaa
      721 attatcaatt tcattagggt tagtaatcat acgtgttacc atgcggtcta tggaagctga
      781 aataagcatt tcaaggttat aataggctgc atcttgatta ccatatttat tctcaatgat
      841 ttcaccttgc tctgttaggc gaattcgatc tttgattgaa ccaaaaggtt gtgatgtgat
      901 agcttcataa gatggaccac caccacgacc aacagttcca ccacgaccgt ggaaaaatgt
      961 gattttaata ccgttttctt cacctatttt tgttagctca ttttgagctt tatatagagt
     1021 ccaaccagat gataaatagc caccatcttt attactatct gaataaccta gcattatttc
     1081 ttgataattg ttgttagtag caatccattt tttaactaat tcataatgta gatactgggt
     1141 cataatatca cgtgaattat ccaaatcttc gatggtttca aataacggta caatctgaat
     1201 acgagcttga ttggcatcaa ttagtccaac ttcttttaac atgatagcta actcaaacat
     1261 atccgaaacg ctctctgtgt gagaaatgat atgctggttt ataatgtctt cacccaattg
     1321 atcttttaac tcacgtgctg tttggaaaat agccaattct ttttgaagta actcggattt
     1381 aggagcatgc gtagaagata atgttctagg atcttctgtt aattctttta agagaagttg
     1441 acatttttct tcttctgaaa gagaactgta atcatctaca atattggcag acttcaataa
     1501 ttctgctaca caagcttcat taatactaga atcttgacgc atatcaatag tagctaaata
     1561 aaatccaaag atatctactg cttgaataag acaagctaat tcgccatcaa caagagaatc
     1621 ttgtccatat tgaactaaag attgcttcac aagtaacaaa tcttgtttaa actcctcagc
     1681 agtagcgtat gctggtagtt cttctgtctg tatgtcagaa gctacttgag cgatatgtga
     1741 ttttatatac cttcctacaa tatcagaaga ttcttggcga ctaacaaatt tctcttttgg
     1801 ggaagaacca acttttaaat ttagtagggt ttgtaccagt ttatcttgga taaaattgaa
     1861 agctttacgg taaggttcat tttccctata taccgaatta tcttgtgact ggtttgccaa
     1921 tgtaacaagt tcgggagaga cttcagttag atttattgaa agtgacatat tacgataaag
     1981 ttcatttagc tgctcaatat aatggttaat aatcacttca ctttgtacca tagctgataa
     2041 tcttagtgtc tcagcagtta caaagggatt gccatctcta tcaccaccaa tccacatccc
     2101 catagtcaga ggcttaggat tctccaagtg aatacctttt ttagctgcca gtgccttata
     2161 ttctgctgtt aatttggtaa ctgctttaat aagagaacgg ttgtaatatt ccattacatt
     2221 ggttatctca tttttgactt tcaatttttt ctcgcgaata gtgtctgttt gcatgataat
     2281 gccgatgtaa cgacgcaagt cagcatacca tttttcttga ttcacgattc ctgccttgac
     2341 atctctgtat ttacgtaata aatcatgaat cttagacgtt aattcgagca ccgttttacg
     2401 ctgcacctga gtaggatgag ctgttagcac aggcacaaca ttgacgtgtt caagaatatc
     2461 tttagcattt tcatggccag ctacaacatc aatagtcgtt gataactttc ctaggtaatc
     2521 ttggttaaga ttatttttat aattaatttc atatgctaaa tcaacgtctt ctgaaatatt
     2581 aattaagagc ggcaaaatag agaagtaacg agaaacaata accatctcct cgttgcttaa
     2641 agccgaaata gtttccttca gaccagtata atcgtcagtt aatgataatg aaactatttt
     2701 ttcaatcttg tcaaatgatt cactaccaat caatttttgc gttgcttcat ccaaaagctg
     2761 cttcagtaaa ccgacctctt cggtaatgat ttctttattg ctactacttt ctaatttcgg
     2821 gtgggacaaa ccatcacttc ctctcttaaa tctcactact atgataccat tttaaagtaa
     2881 aaagtaaacc attctaggat ttttagatag aattttcaga tttttactta tttatgacag
     2941 taatcagttt ttcttgttcg acttttttga tatactgagg ataattgaga ttaactgatt
     3001 aattgttaat taacaactat tattaaagga gtatcgcatg aaacttaaaa aacgctctga
     3061 attccctgaa aatgaacttt gggatctgac tgctctctac aaagatcgtc aagatttttt
     3121 actggctatt gaaaaagctc ttgaggatat caaggttttc aaaaaaaatt acgagggaaa
     3181 acttaattgt gttgaggatt ttactagtgc ccttatggaa atcgagcata tttatatcca
     3241 gatgagtcac attgatacgt atgcttttat gcctcaaact actgacttta gcaacgaaga
     3301 gtttgcacaa atttctcaag caggatcaga cttcgcaact aaagctaatg tactactttc
     3361 tttctttaat actgccctag caaatgcaga tataaagatt ttagattctt tagaaaataa
     3421 ccctcatttc aaagcaacta ttcgccaagc taaaattcaa aagcaacact tattaagtcc
     3481 agaagtcgaa aaagctttga caaacttaaa tgaagtccta aatacaccat atgatattta
     3541 tactaaaatg cgtgctggcg attttgatat ggaagatttt gaagttgacg gaaaaaccta
     3601 caaaaatagt tttgtaactt atgaaaatta ctttcaaaat cacgaaaatg cagagatacg
     3661 tgaaaaatcc ttccgttcat tctcgaaagg tctacgtaag caccaaaatg ctgcagctgc
     3721 ggcttatctt gcaaaagtca aatctgaaaa gttaattgct gatatgcgtg gttacgatag
     3781 tgtttttgat tatcttcttt ccgaacaaga agttgaccgt tcaatgtttg acagacaaat
     3841 tgatcttatc atggatgaat ttggtccagt agctcaacga ttcctcaaac atatcgccga
     3901 tgttaatggt attgaaaaga tgacttttgc agattggaaa ttagacattg ataacgaact
     3961 taatcccgaa gtaagtatca atgatgctta tgacttagta atgaaatcag ttgctccttt
     4021 aggaaaagaa tattctcaag aggttgagcg ctatcaaaaa gagcgttggg ttgattttgc
     4081 agctaacgct aataaagatt ctggaggata tgctgcggat ccttacaaag ttcatcccta
     4141 tgtactcatg agctggacgg ggcgtatgag cgatgtctac acacttattc atgagattgg
     4201 acattctggt caatttatct tctcagataa tcaccaaagt tttttcaata ctcacatgtc
     4261 cacttactat gttgaagctc catctacttt taacgaactt ttacttagtg actacttaga
     4321 aaaccaattt gatacagcac gtcaaaaacg ctttgctctt gcacatcgct taactgatac
     4381 ttacttccac aatttcatta ctcacttact tgaagctgct ttccaacgta aagtttacac
     4441 tctaattgag gagggaggaa cttttggagc agaacaatta aatgctatta tgaaggaagt
     4501 attaacacaa ttctggggag atgctattga aatagatgac gatgccgctt tgacatggat
     4561 gagacaagct cattactaca tggggctcta tagctatact tactctgctg gattagtaat
     4621 ctcgacagcc ggctatctca atctcaaaaa taatccaaac ggagcaaaag agtggttagc
     4681 attcttgaaa tctggtggga gccgtacccc tttagaaaca gctctattaa tcagtgctga
     4741 tatttctact gataagcctc tgcgcgatac tattaatttc ttatctaaca ctgtagacca
     4801 aattattaat tacagtaatc ttattgagca aggctaaact acagcctttc aaaaacataa
     4861 aaacaagttg gagatatctc caacttgttt ttatcttatt ttttgtaaac ttcctgataa
     4921 aattcaattt tgtcattctt tttaacttta atttggtttg ctgccttagg agctaattta
     4981 ttattgacct tgaacatcca ataaagccct ttggtttcat cttggctgat gccatcaatt
     5041 gaagtaatga aaccatcttt ttctttaact ttatagttag ctttaagcac atccaaaaca
     5101 gtatcacctt tatggtaaac aaccttttca cttaacacgt tcgtatcttc tttaaccacc
     5161 aattcaacct taccaacttc taacgttgag ctagttgttg tcttattcga tgtagatggt
     5221 tgcccgcaag ctactaaagt tgtagcgaat actgttagca agaataattt aatgtatttt
     5281 ttcattccta aatctcctta aaatcggtaa caaaagagga taaaataaac aagttgataa
     5341 agcgtgtgcc atattaaaac taagtcctgc taatacataa gtccaccaag gcatatgata
     5401 taaccaagca aaacaggtat caataatgac gccataaaca acccctaacc ctcctgcaaa
     5461 gaaagtttga agtactacct ccgttatttt accaaaacat acagcttttg tcagcggata
     5521 tatcagaaat ttccacaagc ataaaattaa agtgaaggaa atgatttgca aaaagaccca
     5581 tggcccaaag cccattaaaa aagaagaaac tagcattgtt atagttacag tagctacacc
     5641 ttcttttaaa tcgaataaga caactgtaat caaaaaaatg gcagtaattg gctgaatgtt
     5701 aggcagaggc gcaaaagcat atctcaacac aacacacaaa gcagataata tagcgactct
     5761 agtcaattta gttaaagttg aattctttat catgtcttta attatacctc aaaactatga
     5821 ctaccgctta aaagtctttt ttattacaat ttcatttcta atgttacata aacgacctct
     5881 tttttaaaag aacgtaaata agcctgtaaa ctttgatttt ttaaaccttt taagttacaa
     5941 tatcaataat gagaagaaga attaaaccta ttgttgttgc ggtttttttc agtttatttg
     6001 gacttttatt aattattggt catttacatt cgacaaatac tttgaaaaaa gaattagttg
     6061 aagctaaaaa gacaattcca tccgtaaaag cttcaaaagt accgcaaaaa tcaacatcat
     6121 cgaaagataa agagtttgtt cttaaaccga ttatcgatgt ctctggttgg caacttccta
     6181 aggagattga ttacgatacg ctttcaaaaa atatttcagg tgttgttatt cgtgtctttg
     6241 gtggatcaaa gatatctaag actaataacg ctgcttatac aactggaatc gataaatcgt
     6301 ttaagaccca tatcaaagaa tttcaaaagc gaaatatccc agtagctgtc tacagttatg
     6361 cacttggttc aagtgttaaa gaaatgaaag aagaggctca gatattttat aagaatgcag
     6421 ctccttacaa accaactttt tattggattg acgtagaaga ggagacaatg tctaacatga
     6481 ataaaggtgt ccaagcattc cgaaaagaat taaaaagact tggtgctaaa aatgttggta
     6541 tctacattgg tacttacttt atgactgagc aaggcatctc tgtaaaagga tttgacgctg
     6601 tttggattcc aacttatggt agcgattctg gatactatga agcggctccg caaactgaac
     6661 ttaaatacga tttacaccaa tacacctctc aaggttatct accaggattc aatcaaccgc
     6721 ttgatttaaa tcaaattgca gttaataaag acaagaagaa aacttatgag aaactttttg
     6781 gaaaagtaaa agagtaaaag ctactgttaa cagtagcttt tttgataaac tatttattat
     6841 ttaattcttc aatagaaagg attttttggg ttggtttttt cctaagcgta gtaacgattg
     6901 cttctgccac tatcacaacc ttagttggaa agaccttttg tacaacaata cctaagaaag
     6961 gcaaatgact aaataacttt atacacttgg cttggaaaat cgagagaggt cgctcttcac
     7021 catacatcaa acctggtctt acaaaaagat aatccagacc gcttgctttg attatctgct
     7081 ctgccttcct tttactttta atgtaagctg aatagccgct gttggctgaa atataaacta
     7141 actttggtat ttgatttttg tgacagagtg ctactgcttt ttgggttgct ttaacgttaa
     7201 gctcatctag ttgattgggc ttaatcgctc caatacagtc aattaatata tcaaaagttc
     7261 tgtcttctaa atgaatctta tcagcttctg taatatctcc cctaatgtag gttaatctag
     7321 gatccttaaa tatatcacct ttaccttcat gtcttgataa gtaagccact ttatgccctt
     7381 ttgtaagcgc tgcttttatt atctgctttc ctaaaaaacc actaccacct gcaatcagta
     7441 tttccatagt tccctctcct tcattttgta aatttagtat agaaattttt atatgccttg
     7501 tcaaagtaat cgtttaatac ctgaccacgg tatgatttca agtcattaca tcaggacact
     7561 tcatatttag ctatttataa aacttcta
//
LOCUS       DATRMA010000080         7407 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.080, whole genome shotgun sequence.
ACCESSION   DATRMA010000080 DATRMA010000000
VERSION     DATRMA010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7407
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.080"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            195..1427
                     /gene="arcA"
                     /locus_tag="VBC28_001709"
     CDS             195..1427
                     /gene="arcA"
                     /locus_tag="VBC28_001709"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HEO6815672.1"
                     /translation="MTQTHPIHVFSEIGKLKKVMLHRPGKEIENLMPDYLERLLFDDI
                     PFLEDAQKEHDAFAQALRNEGVEVLYLENLAAESLTNQEIREQFIDEYIGEANVRGRA
                     TKKAIRELLLNIKDNKELIEKTMAGIQKSELPEIPSSEKGLTDLVESNYPFAIDPMPN
                     LYFTRDPFATIGNGVSLNHMFSETRNRETLYGKYIFTHHPEYGGKVPMVYEREETTRI
                     EGGDELVLSKDVLAVGISQRTDAASIEKLLVNIFKQNLGFKKVLAFEFANNRKFMHLD
                     TVFTMVDYDKFTIHPEIEGDLRVYSVTYENQDLHIEEEKGDLADLLAKNLGVEKVELI
                     RCGGDNLVAAGREQWNDGSNTLTIAPGVVIVYNRNTITNAILESKGLKLIKINGSELV
                     RGRGGPRCMSMPFEREDL"
     gene            1433..1864
                     /locus_tag="VBC28_001710"
     CDS             1433..1864
                     /locus_tag="VBC28_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO6815673.1"
                     /translation="MKLLVNHLPYQRAASLYIRFSVFVIERNIKMEEEFDDNDEQDTI
                     YAVLYDGKQPVSTGRFLPETQTEARLTRIATLKGYRGNGYGTKIIIALENYAKENGYH
                     YLTIHAELTAKDFYQTLGYQATGNIYMEDGEACQTLEKYLI"
     gene            1880..2893
                     /gene="argF"
                     /locus_tag="VBC28_001711"
     CDS             1880..2893
                     /gene="argF"
                     /locus_tag="VBC28_001711"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000195397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEO6815674.1"
                     /translation="MTQVFQGRSFLAEKDFSREEFEYLIDFSAHLKDLKKRGVPHHYL
                     EGKNIALLFEKTSTRTRAAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRMF
                     DGIEFRGFSQRMVEELAEFSGVPVWNGLTDEWHPTQMLADYLTIKENFGKLEGITLVY
                     CGDGRNNVANSLLVAGTLMGVNVHIFSPKELFPAEEIVKLAEEYAKESGAHVLVTDNV
                     DEAVKGADVFYTDVWVSMGEEDKFKERVELLQPYQVNMELIKKANNDNLIFLHCLPAF
                     HDTNTVYGKDVAEKFGVKEMEVTDEVFRSKYARHFDQAENRMHTIKAVMAATLGNLFI
                     PKV"
     gene            2956..4383
                     /gene="arcD"
                     /locus_tag="VBC28_001712"
     CDS             2956..4383
                     /gene="arcD"
                     /locus_tag="VBC28_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HEO6815675.1"
                     /translation="MEKEKKLGLLPLTMLVIGSLIGGGIFDLMQNMSSRAGLVPMLIA
                     WVITAIGMGTFVLSFQNLSEKRPDLTAGIFSYAKEGFGNFMGFNSAWGYWLSAWLGNV
                     AYAALLFSSLGYFFKFFGNGNNIISIIGASIVIWVVHFLILRGVNTAAFINTIVTFAK
                     LVPVIIFLISALLAFKFNIFSLDIWGNGLHQSIFNQVNSTMKTAVWVFIGIEGAVVFS
                     GRAKKHSDIGKASILALFTMISLYVLISVLSLGIMSRPELANLKTPAMAYVLEKAVGH
                     WGAILVNLGVIISVFGAILAWTLFAAELPYQAAKEGAFPKFFAKENKNKAPINSLLVT
                     NLCVQAFLITFLFTQSAYRFGFALASSAILIPYAFTALYQLQFTLREDKSTPGHQKNL
                     IIGILATIYAVYLIYAGGFDYLLLTMIAYTLGMILYIKMRKDDKLPIFVGYEKISAIV
                     ILALCLLCIIEIMTGQIDIAAILAG"
     gene            4404..5360
                     /gene="arcC"
                     /locus_tag="VBC28_001713"
     CDS             4404..5360
                     /gene="arcC"
                     /locus_tag="VBC28_001713"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEO6815676.1"
                     /translation="MVKEHQKIVVALGGNAILSTDASAKAQQEALINTSKSLVKLIKE
                     GHDVIVTHGNGPQVGNLLLQQAASDSEKNPAMPLDTCVAMTEGSIGFWLQNALNNELQ
                     EQGIDKEVATVVTQVIVDEKDQAFTNPTKPIGPFLSEEDAKKQAQETGSKFKEDAGRG
                     WRKVVPSPKPVGIKEASVIRRLVDSGVVVISAGGGGVPVIEDANTKALKGVEAVIDKD
                     FASQTLSELVDADLFIVLTGVDNVFVNFNKPNQEKLEEVTVSQMKQYITENQFAPGSM
                     LPKVEAAIAFVENKPESRAIITSLENIDNVLAQNAGTQIVAN"
     gene            complement(5470..6495)
                     /gene="trpS"
                     /locus_tag="VBC28_001714"
     CDS             complement(5470..6495)
                     /gene="trpS"
                     /locus_tag="VBC28_001714"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEO6815677.1"
                     /translation="MTKPIILTGDRPTGKLHIGHYVGSLKNRVLLQNEGSYTLFVFLA
                     DQQALTDHAKDPQTIVESIGNVALDYLAVGLDPNKSTLFIQSQIPELAELSMYYMNLV
                     SLARLERNPTVKTEIAQKGFGESIPAGFLVYPVAQAADITAFKANLVPVGTDQKPMIE
                     QTREIVRSFNHAYNCQVLVEPEGIYPENDAAGRLPGLDGNAKMSKSLNNGIFLADDMD
                     TVKKKVMSMYTDPNHIKVEEPGQIEGNMVFHYLDVFGRDEDQKEITAMKEHYQKGGLG
                     DVKTKRYLLDILERELSPIRERRLEYAKDMGQVYQMLQKGSEKAQAVAASTLDEVKSA
                     MGLNYFK"
     gene            complement(6603..7295)
                     /locus_tag="VBC28_001715"
     CDS             complement(6603..7295)
                     /locus_tag="VBC28_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001250491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HEO6815678.1"
                     /translation="MRRDDIIMPQDGRKMAVLLGFIGGFIDVYSHMQFHSLVATQTGN
                     IILMVANLGQNDFHFFITKLFSIFFFSIGFLVGIVIKERAVTAFWRLYALCPLLLSTM
                     FIPFVENVNHNIWVALLAFSTGILMLTFSGSRIESEPYTILMTSGNYRKMLQFWYEYI
                     VNRQRTAMQKRRAINYSLVVLAFIIGALVAAIVYDIFAYRAILGVTITLLIIMIHYTI
                     EIIKNDLTLHNV"
BASE COUNT         2434 a         1319 c         1299 g         2355 t
ORIGIN      
        1 aaaagaaaga aattgtatag aaatcgcttt catttctata aatttaaatt tttttggata
       61 taatcgtgac attcttaaca cttttaatgc gaataacttc cctattttat attcaaaaga
      121 aagtagaatg taaatgtaga tatagagata aatcctgttt caatacaaat ctctaactaa
      181 gaaaggagta aaatatgact caaacacatc caattcatgt tttctcagaa attggaaaac
      241 ttaaaaaagt tatgctacac cgtccaggta aagaaataga aaatctgatg cctgattatt
      301 tagagagact cttgtttgat gatatcccat ttttggaaga tgcacaaaaa gaacacgatg
      361 cgtttgcaca agcacttcgc aacgaaggag tcgaagtact ttaccttgaa aatcttgctg
      421 ccgagtcgtt aacaaaccaa gaaattcgtg aacagtttat cgatgagtac attggcgaag
      481 caaatgttcg cggtcgtgct actaaaaaag ctattcgaga attattactc aatatcaaag
      541 ataataaaga acttatcgaa aaaactatgg caggtatcca aaagtctgag ctacctgaaa
      601 tcccatcttc tgaaaaaggt ctaactgatc ttgtcgaatc taattatcca tttgccatag
      661 atccgatgcc aaatctttac tttacacgtg atccatttgc aactattggg aatggagttt
      721 cattaaatca tatgttctct gaaacacgta atcgcgaaac tttatacggt aaatatatct
      781 ttacacatca cccagagtac ggtggaaaag tccctatggt ctatgaacgt gaagaaacaa
      841 ctcgtattga aggtggagat gagcttgtcc tttctaaaga tgtccttgcc gttggtatct
      901 ctcaacgtac tgatgctgcc tctattgaaa aactcttagt caatattttt aaacaaaatt
      961 taggttttaa aaaggtttta gcatttgaat ttgcaaataa tcgtaaattt atgcaccttg
     1021 acacagtctt tacaatggtt gactatgaca agtttacaat tcacccagaa attgaaggcg
     1081 atcttcgcgt ttactctgta acttacgaaa accaagatct tcatattgaa gaagaaaaag
     1141 gtgatttggc agatcttcta gcgaaaaacc taggagttga gaaagtggaa ttgattcgtt
     1201 gtgggggtga caacttagtc gctgctggac gtgaacaatg gaatgatggt tcaaatacat
     1261 taactatagc accaggtgtc gttatcgttt acaacagaaa tacaatcaca aatgcaatct
     1321 tagagtccaa aggattgaaa ttaattaaga ttaatggaag tgaattagtt cgtgggcgtg
     1381 gtggaccacg ctgcatgtca atgccatttg aacgtgaaga cctttaaaat ttatgaagct
     1441 attagtcaat cacttgcctt accaaagagc tgcaagccta tatattcgct tttctgtttt
     1501 cgttatcgaa agaaatataa aaatggaaga agaattcgat gataatgacg agcaagatac
     1561 tatttatgct gttctttatg atgggaaaca gccagtttca acaggacgct ttcttcctga
     1621 aactcaaaca gaagctcgtt tgactcgaat tgcgacttta aaaggttatc gtggtaacgg
     1681 ctatggtacc aaaattatta tagcactaga aaattatgca aaagaaaatg gttatcacta
     1741 tctaactata catgctgaat tgacagcaaa agatttctac cagacacttg ggtatcaagc
     1801 tactgggaat atttatatgg aagacgggga agcctgtcaa acactcgaaa aatatttaat
     1861 ttaaaggaga cattaacata tgacacaagt atttcaagga cgtagtttct tagcagaaaa
     1921 agatttttct cgtgaggaat ttgaatatct tattgatttt tcagctcatt taaaagacct
     1981 taaaaaacgt ggtgttcctc atcattatct tgaaggtaaa aatattgctc tcttatttga
     2041 aaaaacatct actcgtactc gcgcagcctt tacaactgca gcaattgacc taggcgctca
     2101 tccggaatac cttggtgcaa atgatattca acttggtaaa aaagaatcaa cagaagatac
     2161 tgctaaggtt ttaggacgta tgtttgatgg tattgaattc cgtggtttta gccaaagaat
     2221 ggttgaagag cttgctgaat tttctggagt acctgtctgg aatggtttaa cagatgaatg
     2281 gcatccaaca caaatgctag ctgactacct tactatcaaa gaaaacttcg gtaaacttga
     2341 aggtattact cttgtttact gtggtgacgg acgtaacaat gttgccaact cgcttttagt
     2401 ggctgggact ttgatggggg tcaatgtaca catcttttct ccaaaagaac ttttccctgc
     2461 tgaagagatt gttaaattgg ctgaagaata tgccaaagag tccggggctc acgttctcgt
     2521 tactgataat gtagacgaag ctgtaaaggg agcagatgtc ttttacactg atgtctgggt
     2581 atcgatggga gaagaagata agttcaaaga acgcgttgaa cttcttcaac catatcaagt
     2641 aaacatggaa ctgattaaaa aagctaataa tgataatctt atcttcttac actgcttacc
     2701 tgcattccat gatacaaata ccgtttacgg caaagacgtc gctgaaaaat ttggcgtcaa
     2761 ggaaatggaa gttactgatg aagtcttccg tagcaaatat gctcgtcatt tcgaccaagc
     2821 tgaaaatcgt atgcacacta ttaaagctgt aatggctgca acccttggaa atcttttcat
     2881 tccaaaagtt taaattaaac gactataggg agttgacata tagtcaattc cctattttag
     2941 gaaatgaggt ataaaatgga aaaagaaaaa aaattaggtc ttttaccact aacaatgctt
     3001 gtcattggct ctcttatcgg tggcggaatc tttgatttaa tgcaaaatat gagttccaga
     3061 gccggtttgg taccaatgct tattgcttgg gtaattactg ctatcgggat ggggactttc
     3121 gttttaagtt ttcaaaattt atctgaaaaa aggccggacc taacagctgg aatctttagt
     3181 tacgctaaag aggggtttgg aaactttatg ggatttaact ctgcatgggg ttattggtta
     3241 tcagcttggc ttggaaatgt tgcctacgct gcactcttat tcagttcact cggttatttc
     3301 tttaaattct ttggtaatgg aaataatatc atctcaatta ttggagcaag tatagttatt
     3361 tgggttgtcc atttcttaat tttaagaggt gttaatacag ctgcatttat taataccata
     3421 gttacctttg caaaattagt acctgttatt attttcttaa tttcagcgtt attagctttc
     3481 aaatttaaca tttttagtct tgatatctgg ggaaatggat tacatcaatc aattttcaac
     3541 caagtcaatt caactatgaa aaccgctgtt tgggtattta ttggtattga gggcgccgtt
     3601 gtcttctcag gtcgtgctaa aaaacactct gatattggta aagcaagtat cctagcatta
     3661 ttcactatga tttcacttta tgtattgatt tctgttttat cacttggtat catgtcacgt
     3721 ccagaacttg caaacttaaa aacaccagct atggcttacg ttctagaaaa ggctgttggt
     3781 cactggggcg ctatcttagt taaccttggt gttatcattt cagtatttgg cgctattctt
     3841 gcttggactt tatttgcagc agaattacca tatcaagctg ctaaagaagg tgcttttcct
     3901 aaattctttg caaaagaaaa taaaaacaaa gctccaatca attcactctt agtcactaat
     3961 ctttgtgtac aagcattctt aatcacgttc ttattcacac aaagtgctta tcgttttggt
     4021 ttcgcattag catcatctgc tatcttaatt ccttatgctt ttacagcact atatcaatta
     4081 caattcacac tccgtgagga taagtcaact ccaggacatc aaaagaattt aattatcggt
     4141 atcctcgcta caatctatgc tgtttacctt atctacgctg gtggttttga ttacttactt
     4201 ttgacaatga ttgcttacac tctaggtatg attctctata ttaaaatgag aaaagatgac
     4261 aagcttccta tctttgtggg atatgaaaaa attagtgcca ttgtcattct agcgctttgc
     4321 ttattatgta ttattgaaat tatgactgga caaattgaca ttgctgcaat cctagcaggt
     4381 tagtcattag aaagagggat aaattggtaa aagaacatca aaaaatcgta gttgccttag
     4441 gtggcaacgc tattctatca acagatgctt cagcaaaagc acaacaagaa gcattaatca
     4501 acacatcaaa atcactggtc aaactgatta aagaaggcca tgatgtcatt gttactcatg
     4561 gaaatggtcc ccaagttggt aacttattgc tacagcaagc agcttcagat tctgaaaaga
     4621 atccagcaat gcctttggat acttgtgttg ccatgacaga aggaagtatt ggtttttggt
     4681 tacaaaatgc tcttaacaat gaattacaag aacaaggcat tgacaaagag gttgcaacag
     4741 tggtaactca agttattgta gatgaaaaag atcaagcttt cactaatcca acaaaaccta
     4801 ttggcccatt cttatctgaa gaagatgcca agaaacaagc ccaagaaaca ggttcaaaat
     4861 ttaaagaaga tgctggccgt ggatggcgta aagtcgttcc ttcaccaaaa ccggttggta
     4921 tcaaagaagc ttctgttatt agacggttag tggactctgg tgtggttgtt attagcgcag
     4981 gtggcggagg tgtaccagtt attgaagatg ctaatacaaa ggcattaaaa ggggttgaag
     5041 ctgttattga caaagacttt gccagtcaaa ctttatcaga acttgttgat gctgacttat
     5101 tcatcgtctt aactggcgtt gacaacgttt ttgttaattt taataaacct aatcaagaaa
     5161 aattagaaga agttactgtt tcacaaatga aacaatatat cactgaaaat caatttgccc
     5221 ctggaagtat gctaccaaag gtcgaagctg cgattgcttt tgttgaaaat aaacctgaat
     5281 cacgagctat tattacttca cttgaaaata ttgataatgt tttagcccaa aatgcaggga
     5341 cacaaattgt tgcaaattaa acaatgtaga ttaccattac atacactcct tattaatgtc
     5401 actccttagg cattcctata ataaaagaga gctagatata tctagctctc ttttattata
     5461 ggacatatat tatttaaaat agttcaaacc catggctgat ttaacctcat ccaaagtaga
     5521 ggctgcaaca gcttgagctt tctcactacc tttttgtaac atttgataaa cttgtcccat
     5581 atctttagca tattcgaggc gacgttctct aataggtgat aattcacgct ctaagatatc
     5641 aagtagataa cgttttgttt taacatcccc aagtccacct ttttgatagt gttctttcat
     5701 agcagtaatc tctttttggt cttcatctct tccaaaaaca tcaaggtaat gaaataccat
     5761 attaccttcg atttgtcctg gttcttctac tttaatgtgg tttggatcag tatacatact
     5821 cataactttt ttcttaactg tatccatatc atctgctaaa aagattccat tatttaatga
     5881 cttagacatt tttgcattac catctaaccc tggcagacga cctgctgcat cattttctgg
     5941 atagattccc tctggttcaa ctaaaacctg acaattataa gcatgattga aagatctaac
     6001 tatttcacgt gtttgttcaa tcattggttt ttggtcagtc cctacaggaa caaggttagc
     6061 tttaaaagcg gttatatcag ctgcttgtgc aacaggataa actaagaatc ctgctggaat
     6121 agactcacca aaaccttttt gagcaatttc tgtttttaca gttggattac gttctaaacg
     6181 tgcaagtgaa accaagttca tataatacat acttaattct gctaactcag gaatttgact
     6241 ttgaatgaat aaagtcgact tgtttggatc taacccaaca gctaaataat caagtgcaac
     6301 attaccaatt gattcaacaa tagtttgcgg atctttagca tggtctgtca gtgcttgttg
     6361 gtctgctaaa aagacaaata aagtatagct accctcattt tgaagtagaa ctctattttt
     6421 taagctacct acataatgtc caatatgcaa ctttcctgtc ggtctatcac ctgttaagat
     6481 aattggtttt gtcatgttta ctccttaata taccaaagat atcatttcat atagttatct
     6541 tatcatgtca caagccctat tgcaattcat ccctacaaga aacattcacc ttattaagat
     6601 gattaaacat tatgtaacgt taaatcgttc ttaataattt caatcgtata atgaatcata
     6661 ataattaaaa gcgttatcgt cacacctaaa atagctctat atgcaaaaat atcgtaaact
     6721 attgcagcta ccaatgctcc aataataaat gctagaacta ccagtgaata attgatagct
     6781 cgtctttttt gcatagccgt tctctgacga ttaacaatat actcatacca aaattgtagc
     6841 attttccgat aattaccaga agtcattaag atagtatatg gttccgattc aatgcgactt
     6901 cctgaaaatg tcaacattaa aatacctgtg ctaaaggcta ataaagctac ccagatattg
     6961 tggtttacat tttcaacaaa aggaataaac atcgtagaaa gtaaaagggg acataatgca
     7021 tatagacgcc aaaaagctgt tactgcacgt tctttaatca caataccaac caaaaaacca
     7081 attgaaaaaa agaaaataga aaataattta gtgataaaaa agtgaaaatc attttgtccg
     7141 agatttgcca ccatcaagat aatattacct gtctgtgttg caacaaggga gtgaaactgc
     7201 atgtgactat aaacgtcaat aaaaccacca ataaaaccca ataatacagc catttttctt
     7261 ccatcttgtg gcattataat atcatctctg cgcattatag caaattctcc ttcttgtatt
     7321 atcatgtttt ctattatata ccaataaaat aggcattcaa ctcaaaaata tcgagaaatt
     7381 tcttttctaa atttacttga ctaccaa
//
LOCUS       DATRMA010000081         7223 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.081, whole genome shotgun sequence.
ACCESSION   DATRMA010000081 DATRMA010000000
VERSION     DATRMA010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7223
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.081"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            complement(4..3213)
                     /locus_tag="VBC28_001716"
     CDS             complement(4..3213)
                     /locus_tag="VBC28_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001060281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6815679.1"
                     /translation="MNNKGVGGDGVQIYQYYIKMDNNKPYLSPKDKTTVEKLEDRWKK
                     ITFKVQDTGIGLKDVYLQSVKYVGGGNNNLDLITPPGFKKEDKKVEKPKLDRPPGIDL
                     PAPTSMRSFDYSTPPGTKPSKPKDSLSTPPGFPDLNTPPDEAPKDSKKDAIEDKSGAI
                     KYAKSLQLSFVDDPILASKVNGKILQVESDGKLVIPRNALSANQFDDTSLKIHRNNNR
                     NKEITITTDYFADTKYVNITAVDYLSNTTFEQLATGETVDYHAIVFSSFAAIKDKGGK
                     IYVNDKLQETSRIALKDKSVKIGIELPNDVRHIDSLSVRRLNEVKTVDNILKNDEQDI
                     NLSKTYQLKYNPTNRRLEFTINNINSSSEIMTTFKDGKMPELVEQKDVSLDINDMDMS
                     KFKTIRLGRKDSEFKGQLIAKTGTVELDMFFKQSQDPASIIKKIYLIQNGVPNELKKF
                     DSSFGLTESQIDGYYIYKDAINLKFKLTSGASLKVVYKGQEDPYSHQKEDMTKKGEQL
                     SHSTQANENTAKVTFANIDWSHYSKVTVNGKEVVKGSELPLTKGWTTFVLHKTENSLN
                     VKSLIMETGSVSKKVQQLPLSPRLSKNKHMRDMLLTMQKDSAYYETSDSLVLRINLTA
                     DTKLNFNAVKGASALTENMMMRQFAVAGPQDDPVSEHKYPSVFLLTPALLETASEATL
                     NGKEITASGIIGHIKDGDKSKHVEVKMVNENGDMLGTPVIIQGKDLTNRTKPLMSGRR
                     VLYAGKQYEFRAKLPLSRFNTWIRVEVVTEAGEKASIVRRMFFDQSVPELNTAVAKRD
                     LTSDTALIHIVAKDDSLKLKLYQDDSLLESVDKTGLYSFRNGVEITKDMTVPLEFGDN
                     IIKLSAVDLSNYRRNETLHIYRNRFDVKASQMTADKGAKVTVDMLMKHLVVPEMAGAY
                     TLTIDEAPNTNESGMLTNAKVSIHYVNGGVDKVDVPIKVVDLEAIRKAEEARKAEEGH
                     KTQEAPIVEEGYKVNNVHQTDTTVKASDLPKTKTVSAVHMARTDNKQITSHQTHVEKQ
                     IKNTLPSTGDSKRGYYITGMAIVMLSVLFSLAKKFKSKY"
     gene            complement(3259..5916)
                     /locus_tag="VBC28_001717"
     CDS             complement(3259..5916)
                     /locus_tag="VBC28_001717"
                     /inference="COORDINATES: protein
                     motif:HMM:NF017709.1,HMM:NF018035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEO6815680.1"
                     /translation="MTKPQGAVAEKATPAVPKPQKVRVIVEFNKESILDYATEQKKTV
                     AQLNQADVEKKLQSIKQEQDKVLKNIEKSVHFDSSKVKRYDAIINGVALDIQAQEIEK
                     LKTIADVRRVYVSQEYVQTKPLLSSSGQLIGLPEVWNNSQYKGEGTVVAVIDSGVDFK
                     HQALKIKEPNRAKYNKTSIEKLIHEKNLKGKFYSEKVPYGYNYYDYNDNLKDSYGVMH
                     GMHVTGIVGANDDNQKLYGVAPNAQILAMKVFSDDQQNPTTFTDVWLKALDDAILLKA
                     DVVNMSLGTPAGFVHEGKDYPELEVIARACKAGIVIAVAAGNEGNITDGNTYGVKPLA
                     ENYDTALIANPALDDNTLAVASMENLKKHAHVLKFKDKKSGTEVTEVINLHVAPNASK
                     TIIGLAVDLGAGAPSELSKHFDLSGKIAMLEIPEDNKSNGFLEKVQAITKLNPAAILL
                     YNNAKVKDDLGSQLLVESEAAKFNIARITRSTYNNIKNNSNKIITILTERQAIDNSLA
                     GQLSSYSSWGPTPDLRLKPEITAPGGHIFSTVEDNQYADKSGTSMAAPQVAGAAAVLK
                     QYITDKKIPVDNAADFIKLLLMNTAQPIINKQSKDGKTPYFVRQQGSGAMNLAKALVT
                     TVVATVTGTNDNNADGKLELRELKEKKFKARILLRNFGKTNKTYIISSEAIADPVDEK
                     GFRTQNSEHLVSKKADAVTRKVTVEAGKTLAVDLDVDYSDAEALTRNNFLEGYLNLKD
                     TEGVADLHLPFLGFYGSWTEQKAIDAFEGISEIGNGDKKRRVQFYVNKETNKTSSTFT
                     TNGMLSLPIYNNTVFFSPNSPFYDKAGVRIAALRNMEYVQYSIIDPDTNKEVRVLGRS
                     HDVRKLYRLDYRNSFAMMPDSIWDGKIKD"
     gene            complement(5968..6483)
                     /locus_tag="VBC28_001718"
     CDS             complement(5968..6483)
                     /locus_tag="VBC28_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6815681.1"
                     /translation="MTFYKKTDKFGFRKSKVCRSLCGALLGTVAVVSLATASTEIHAD
                     EATTSPTTVTKVPQPVQADTTALNTSKTHSTQATTTPVEAKENKVVKSETVQSESRVM
                     PRDKVVERPETVKASVNSDVSQPITTTPPTINEKTVEIPNLAQDTKKVAPKVTVTPEV
                     VNQPKQLPKYE"
     gene            complement(6517..6858)
                     /locus_tag="VBC28_001719"
     CDS             complement(6517..6858)
                     /locus_tag="VBC28_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6815682.1"
                     /translation="MSEFLSAKPIGHNDSSNTILRIIENTKYIHHYDGKVVVEIPPQK
                     EPIKVFFMDSRLHYLEFSDICRYVTVVLKNSTVDNITNLGLDNHIVYDNGDDMVDPLP
                     SIQHGLFLNDD"
BASE COUNT         2078 a         1355 c         1167 g         2623 t
ORIGIN      
        1 tcattaatat ttgcttttaa actttttagc taaactaaat aatacactca gcataacgat
       61 agccattcca gtgatataat aaccacgttt gctgtcacca gtggatggca atgtattttt
      121 aatttgtttt tcaacatgtg tctgatgtga agttatctgt ttattgtctg ttctagccat
      181 atgaactgcg gaaactgtct tagtctttgg taaatcagac gctttaactg tagtatcagt
      241 ttgatgaacg ttattaacct tgtagccttc ttcaactata ggtgcttctt gggttttatg
      301 tccctcttca gctttacgtg cttcttcagc tttacgaata gcttctaagt caactacttt
      361 aatcggaaca tcaactttat caacaccacc atttacataa tgaatcgata ctttagcgtt
      421 tgttaacatt cctgattcat ttgtgtttgg agcttcgtcg attgttaatg tataagctcc
      481 tgccatttct ggaacaacta agtgcttcat caacatatcc acagttactt tagctccttt
      541 gtcagctgtc atttggcttg ctttaacatc aaaacggttt ctatagatat gaagggtctc
      601 attacgacga taatttgata agtcaacagc agataactta ataatattat ctccaaattc
      661 tagtggtact gtcatatctt tagtgatttc tacaccattt ctaaaactat aaagaccggt
      721 tttatcaaca gattcaagta atgaatcatc ttgatataat tttagtttta gagagtcatc
      781 tttggcaacg atgtggataa gagcagtatc agaagtcaaa tcacgtttag caactgctgt
      841 gttaagctct ggaactgatt ggtcaaagaa catgcgacga acaatacttg ctttctctcc
      901 tgcttctgtt accacttcaa ccctaatcca agtgttaaaa cgactaagtg gtaatttagc
      961 ccggaactca tattgtttac cggcataaag tactctacgt ccactcatta atggttttgt
     1021 tcgattagtc aagtctttac cttgaataat aacaggggtt cctagcatgt ctccattttc
     1081 attcaccatt ttgacttcaa catgcttgct tttatcacca tccttgatgt gaccgataat
     1141 accagatgct gtgatttcct taccatttag agttgcctca ctagcagttt ccaataaggc
     1201 aggagttaag agaaatactg atgggtattt atgttcacta acaggatcat cttgtggtcc
     1261 agcaactgca aactgtctca tcatcatatt ttcagtaaga gcactcgctc ctttaacagc
     1321 attaaaatta agtttagtat ctgcagtgag attaattcga aggactagac tgtcacttgt
     1381 ttcgtaatac gctgaatctt tttgcatagt aagtagcata tccctcatat gcttattttt
     1441 agataatcta ggacttaaag gaagttgttg aactttctta cttacactac ccgtctccat
     1501 aatcaaactt ttaacattta atgaattttc tgttttatgt aatacaaatg ttgtccatcc
     1561 tttagttaaa ggtaactcac tacctttaac aacttctttt ccattcacag taaccttact
     1621 ataatgtgac cagtcaatat tagcaaaggt tacttttgct gtattttcat tggcttgagt
     1681 tgaatgactg agctgttcac cttttttagt catatcttct ttctgatgac tatatggatc
     1741 ttcttgccct ttataaacaa ctttaagact tgcaccactg gttaatttaa atttaaggtt
     1801 aattgcatct ttataaatat agtatccatc tatctgactt tcagttaaac caaaactaga
     1861 gtcaaatttt ttcaattcat ttggaacacc attttggata aggtatattt ttttaataat
     1921 tgaagctggg tcttgagatt gtttgaaaaa catatctaat tcaactgttc cagtttttgc
     1981 aataagttgt cccttaaatt cagaatcctt tcgtccaagt cgaatagttt taaacttact
     2041 catgtccata tcgtttatat ccaaagaaac atctttttgt tcaaccaatt ctggcatctt
     2101 tccatctttg aaagtggtca tgatttctga acttgagtta atgttattaa tagtaaactc
     2161 tagacgacga tttgtcgggt tgtattttaa ttggtaagtt ttgctgagat taatgtcttg
     2221 ttcatcattt ttcaagatat tatcaacagt tttaacctca ttcaaacgac gaacagataa
     2281 actatcaata tgtctgacat catttggtaa ttcaatacca atcttaacag atttatcttt
     2341 aagcgctata cgagaagttt cttgcaattt atcgttaaca taaatcttac cacccttgtc
     2401 tttaatagca gcaaagcttg aaaatacaat ggcatggtaa tctactgttt caccagtagc
     2461 taattgctca aaagtagtat tgctcaaata gtcaaccgct gtgatattga catattttgt
     2521 atctgcaaaa taatctgttg tgatagtaat ttctttattg cgattattat tacgatgaat
     2581 tttaagacta gtgtcatcaa attgattagc tgacaaagca tttctaggaa tgactaattt
     2641 gccatcagat tcgacttgta atattttgcc atttactttg ctagctaaaa tagggtcatc
     2701 aacaaagcta agttgaagag acttagcata tttaattgct cctgatttat cttcaatagc
     2761 gtctttttta ctatcctttg gtgcttcatc cggcggcgtg tttaaatctg ggaaacctgg
     2821 aggagttgat aaactatctt tgggtttgct tggcttagtt cccggtgggg ttgaataatc
     2881 aaaacttctc attgaagttg gtgctggtaa atcaattcct ggtggacggt ctaattttgg
     2941 tttttcaact tttttatctt cttttttaaa tcctggaggt gtgataaggt ctaaattatt
     3001 attgccacca ccaacatact taacagattg aagataaacg tctttcaaac caatgccagt
     3061 atcctgaact ttgaaagtaa tttttttcca gcgatcttct aacttctcta cagtagtctt
     3121 atctttggga cttaagtaag gtttattgtt gtccattttg atatagtatt gataaatttg
     3181 gacaccatcg ccaccgacac ctttattatt caactgtact ttaatttgat aaatgtattg
     3241 tttatcccct tttgcaattt aatctttaat cttaccatcc caaatagaat ctggcatcat
     3301 ggcaaaacta ttacgataat ctaggcgata aagttttcta acgtcgtgtg atctaccaag
     3361 tacacgaact tctttattgg tatctggatc tataatagaa tattgaacat actccatatt
     3421 acgcaaagct gcaatacgaa ctcctgcttt gtcatagaaa ggactattag gagagaaaaa
     3481 tactgtattg ttatagattg gaagactcaa catgccgtta gttgtaaagg ttgatgaggt
     3541 cttgttggtt tctttattaa catagaattg aacgcgacgt tttttgtcac cgttaccaat
     3601 ttcagaaatg ccttcaaaag catcaatagc tttttgctct gtccatgaac cgtaaaaacc
     3661 taaaaatggt aagtgtaaat cggctacacc ttcagtatct tttaagttaa gataaccttc
     3721 caagaaatta ttgcgagtta aagcctctgc atcagaataa tccacatcaa ggtcaacagc
     3781 taatgtctta cctgcttcta cagtaacttt ccttgtaact gcatcagctt tttttgatac
     3841 taggtgttct gaattttgcg tacgaaatcc tttttcatca actgggtccg caatcgcttc
     3901 tgatgagatg atatacgttt tattagtttt tccaaagtta cgaagtagaa tgcgtgcttt
     3961 aaattttttc tcttttaatt cacgtaactc caacttacca tctgcattat tatcgttagt
     4021 ccctgttaca gttgcaacga ctgtcgtaac aagagctttg gcaagattca ttgctccgct
     4081 accttgttgg cgcacaaagt aaggtgtttt gccatctttt gattgtttat tgataattgg
     4141 ctgtgcagtg ttcatcaaga gtaatttaat aaaatcagca gcattatcaa caggaatttt
     4201 tttatctgta atgtattgtt ttaaaactgc agctgcccca gctacctgag gtgcagccat
     4261 tgatgttcct gatttatcag catattgatt atcctccaca gttgagaaaa tatgaccacc
     4321 tggagctgta atctcaggtt ttagacgtaa gtctggtgta ggtccccaag aagaataaga
     4381 agataattgt cctgctaaac tattatcaat agcttgacgc tctgttaaaa tagtaataat
     4441 tttattgcta ttatttttga tattgttata cgtgcttctg gtaatgcgag caatattaaa
     4501 tttagctgct tctgattcaa ctaataattg actgcctaaa tcatctttaa ccttcgcatt
     4561 attgtaaagc aagatagcag ctggatttaa cttagtaatc gcctgtactt tttctaaaaa
     4621 tccattagat ttattatctt ctggaatttc aagcatggcg attttaccac ttaaatcaaa
     4681 atgttttgac aattccgaag gagctcctgc tcccaaatct acagccaatc caataatagt
     4741 ttttgaagcg tttggggcaa catgaagatt aatgacttct gtaacttctg ttccagattt
     4801 tttatcttta aatttcaaaa catgagcatg ctttttaagg ttctccatag aggcaacagc
     4861 caaggtatta tcatcgaggg ctgggttggc aatcaaggct gtatcataat tttctgctag
     4921 cggcttaaca ccataagtat taccgtcagt gatatttcct tcattacctg ctgctactgc
     4981 aatgacgatt cctgccttac acgctcttgc aataacttca agttcaggat agtctttccc
     5041 ctcatgtaca aatccagcag gtgttccaag actcatatta acaacatcag cttttagtaa
     5101 aatagcatcg tctagagctt ttagccacac atctgtgaag gttgtgggat tttgttggtc
     5161 atctgaaaat accttcatcg ctaaaatttg agcatttggt gcaacaccat acaatttttg
     5221 attatcatca ttagctccta cgatacctgt tacatgcata ccatgcatta caccgtagct
     5281 atccttgagg ttatcattat agtcataata gttataacca taaggtactt tttcggaata
     5341 gaattttcct ttgagattct tttcatgaat cagtttttca atactagttt tattatactt
     5401 agcacgattt ggttctttga tttttagtgc ttggtgtttg aaatcaaccc ctgaatcaat
     5461 aactgcaaca acagttccct cacctttata ctgggagttg ttccaaactt ctggcaaacc
     5521 aataagttgc cctgaggaag atagtaaagg ttttgtctga acatattctt gtgagacata
     5581 gacccttcta acatcagcaa tcgtttttaa tttttcaatt tcttgagctt gaatatcaag
     5641 tgcgacacca ttaataatag catcgtaacg cttcactttg cttgaatcaa agtgaacact
     5701 tttttcaata ttcttaagaa ctttatcttg ctcttgcttg atgctttgta attttttctc
     5761 aacatcagct tgatttaact gtgcaactgt ttttttctgt tctgtcgcat aatctaagat
     5821 agattcctta ttaaattcaa caattactct tactttttga ggctttggca cagccggagt
     5881 agctttctca gcaaccgccc cctgtggttt agtcacagta actgccttag ttgcttctgt
     5941 actcttattg cctttctctg agttttctta ctcgtattta ggcaactgct ttggctgatt
     6001 aacaacttct ggtgtaacag ttacttttgg agctaccttc ttagtatctt gagctaagtt
     6061 aggaatctca actgtttttt cattaattgt tgggggtgtg gttgtaatag gttggctaac
     6121 gtctgagtta acactagctt ttactgtttc tggcctttct actaccttat ctctaggcat
     6181 tactcggctc tcagattgaa cagtttcact tttgacaacc ttattctcct tagcttcaac
     6241 aggagttgta gttgcttgtg tactgtgagt cttagaggta tttagagctg ttgtgtctgc
     6301 ttgaactggc tgaggtactt tcgttacagt ggtaggtgag gttgttgctt catcagcgtg
     6361 aatctctgta cttgctgtag ctagactaac aactgctact gtacccaata aagcaccgca
     6421 taggctgcgg cacactttac ttttcctaaa tccaaatttg tccgtctttt tataaaatgt
     6481 catggcttcc tcgtttctaa atttttgtct acatctttaa tcatcattaa gaaataatcc
     6541 atgctgaata ctcggcaacg gatcaaccat atcatcacca ttgtcataaa caatatgatt
     6601 atctagccct aaattggtta tattatcaac ggtactattt ttcaaaacaa cagttacata
     6661 ccgacatatg tctgaaaatt ctaaataatg aagacgtgaa tccataaaaa agactttgat
     6721 aggctctttt tgaggaggaa tttcgacaac aacttttcca tcatagtgat gaatatattt
     6781 cgtattctct ataattctta aaatagtatt agatgaatca ttatgaccaa taggtttggc
     6841 tgaaaggaat tcactcataa aagaacctca cattacttaa ccggtttgtt aaccagctaa
     6901 cgaaatttta gcactcctct aagtacttgt caatggcaaa tgttaagtta aaatcgtttt
     6961 cagcaataag ttcttttatc tttttttaca ttagaaaata tttcagtttt tattatttct
     7021 aattattatc acaaaaaaac acctctattt tctgattttc tcataaaatg tggaaatgat
     7081 cagtttatca tatcaactct aaccataatt gttaaagact acccaaggta ttataaaaag
     7141 cccttgaaat acttcaaagt gctttttata gatgcatatg taggaacaac atgttgcgag
     7201 ttagttatct ggtaataatt ctt
//
LOCUS       DATRMA010000082         7194 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.082, whole genome shotgun sequence.
ACCESSION   DATRMA010000082 DATRMA010000000
VERSION     DATRMA010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7194
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.082"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            76..798
                     /locus_tag="VBC28_001720"
     CDS             76..798
                     /locus_tag="VBC28_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEO6815683.1"
                     /translation="MRLDKFLVECGLGSRTQVKLILKKKQISVNGNSETSPKVQVDEY
                     RDEIKYNGTLVSYEKFVYYMLHKPKGVISATDDPSHKTVLDLLDKTARDKAVFPVGRL
                     DIDTTGLLLLTNNGELAHKMLSPKKHVDKCYEVKISGIMTEDDILAFDKGIILKDFTC
                     LPALLEIVEVNQVKKQSLVKITIKEGKFHQVKRMVAACGKEVLELKRLRMGNLQLDKQ
                     LESGQWRRLTIKEIEKLEKYMQ"
     gene            883..2925
                     /locus_tag="VBC28_001721"
     CDS             883..2925
                     /locus_tag="VBC28_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEO6815684.1"
                     /translation="MKRKYFILNTVTVLTLAAAMNTSSIYANSTETSASVVPTTNTIV
                     QTNDSNPTAKFVSESGQSVIGQVKPDNSAALTTVDTPHHISAPDALKTTQSSPVVEST
                     STKLTEETYKQKDGQDLANMVRSGQVTSEELVNMAYDIIAKENPSLNAVITTRRQEAI
                     EEARKLKDTNQPFLGVPLLVKGLGHSIKGGETNNGLIYADGKISTFDSSYVKKYKDLG
                     FIILGQTNFPEYGWRNITDSKLYGLTHNPWDLAHNAGGSSGGSAAAIASGMTPIASGS
                     DAGGSIRIPSSWTGLVGLKPTRGLVSNEKPDSYSTAVHFPLTKSSRDAETLLTYLKKS
                     DQTLVSVNDLKSLPIAYTLKSPMGTEVSQDAKNAIMDNVTFLRKQGFKVTEIDLPIDG
                     RALMRDYSTLAIGMGGAFSTIEKDLKKHGFTKEDVDPITWAVHVIYQNSDKAELKKSI
                     MEAQKHMDDYRKAMEKLHKQFPIFLSPTTASLAPLNTDPYVTEEDKRAIYNMENLSQE
                     ERIALFNRQWEPMLRRTPFTQIANMTGLPAISIPTYLSESGLPIGTMLMAGANYDMVL
                     IKFATFFEKHHGFNVKWQRIIDKEVKPSTGLIQPTNSLFKAHSSLVNLEENSQVTQVS
                     ISKKWMKSSVKNKPSVMAYQKALPKTGDTESSLSPVLVVTLLLACFSFVTKKNQKS"
     gene            3035..3613
                     /locus_tag="VBC28_001722"
     CDS             3035..3613
                     /locus_tag="VBC28_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEO6815685.1"
                     /translation="MKVKNKILTMVALTVLTCATYSSIGYADTSDKNTDTSVVTTTLS
                     EEKRSDELDQSSTGSSSENESSSSSEPETNPSTNPPTTEPSQPSPSEENKPDGRTKTE
                     IGNNKDISSGTKVLISEDSIKNFSKASSDQEEVDRDESSSSKANDGKKGHSKPKKELP
                     KTGDSHSDTVIASTGGIILLSLSFYNKKMKLY"
     gene            complement(3647..4888)
                     /locus_tag="VBC28_001723"
     CDS             complement(3647..4888)
                     /locus_tag="VBC28_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEO6815686.1"
                     /translation="MVLQDFDNLLKKYAQLIISKGLNVQKGHTLALTIDVEQVHLARL
                     LTEAAYEKGASEVIVDYTDDFITRQRLLHASDEVLTNVPQYTVDKSLALLNKKASRLV
                     VKSSNPNAFATVDPKRLSETTRATAIALEEQSRAIQANKVSWNVAAAAGREWAALVFP
                     ELKTSDQQVDALWDTIFKLNRIYEDDPIAAWDAHEAKLLEKATRLNQEQFDALHYTAP
                     GTDLTLGMPKNHIWEAAGSLNAQGETFIANMPTEEIFSAPDYRRADGYVTSTKPLSYA
                     GVIIENMTFTFKDGKIINVTAEKGQETVQRLIEENDGARSLGEVALVPHKTPISLSGL
                     IFFNTLFDENASNHLAIGTAYAFNVEGGTEMTSQELDEAGLNRSSTHVDFMIGSEQMD
                     IDGIRADGTAVPIFRNGEWAI"
     gene            5085..5213
                     /locus_tag="VBC28_001724"
     CDS             5085..5213
                     /locus_tag="VBC28_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HEO6815687.1"
                     /translation="MAFGDNGQRKKTGFEKLTLFVVILMVLVTVGGLVFGAISAIM"
     gene            5274..6587
                     /gene="obgE"
                     /locus_tag="VBC28_001725"
     CDS             5274..6587
                     /gene="obgE"
                     /locus_tag="VBC28_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEO6815688.1"
                     /translation="MSMFLDTAKISVKAGRGGDGMVAFRREKYVPNGGPWGGDGGKGG
                     SVIFKVNEGLRTLMDFRYNRNFKAKAGEKGMTKGMHGRGAEDLIVSLPPGTTVRDATT
                     GKVITDLVEHDQEFVVARGGRGGRGNIRFATPRNPAPEIAENGEPGEERELQLELKIL
                     ADVGLVGFPSVGKSTLLSVVSAAKPKIGAYHFTTIVPNLGMVRTKSGDSFAMADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSASEGRDPYDDYVSINNELETYNLRLME
                     RPQIIVANKMDMPDSEENLAAFKEKLAANYDEFDDMPMIFPISSLAHQGLENLMDATA
                     ELLANTEEFLLYDETDMQEDEAYYGFNEDERPFEITRDDDATWVLYGDKLEKLFVMTN
                     MERDESIMKFARQLRGMGVDEALRERGAKDGDIVRIGNFEFEFVD"
     gene            6613..6771
                     /locus_tag="VBC28_001726"
     CDS             6613..6771
                     /locus_tag="VBC28_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6815689.1"
                     /translation="MGDKPISFRDKDGNFVSAADVWNAEKLEELFNTLNPNRKLRLER
                     EKLAKEKG"
     gene            complement(6863..7189)
                     /locus_tag="VBC28_001727"
     CDS             complement(6863..7189)
                     /locus_tag="VBC28_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001923513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6815690.1"
                     /translation="MHFIKEHMTYHVNFIDDFLDIKWEKVSNIHLRFWTTIIAYLVIF
                     ILSISTVILNLVLLFQGFLTQNPIIYLLFFITLVCAFYFAYKFITYTPTIVKNALQYI
                     KKLKNV"
BASE COUNT         2462 a         1131 c         1426 g         2175 t
ORIGIN      
        1 aaaagtagta ataaaggatg agtatggaaa aatcctttac aatatatcat tatcgaaaga
       61 ggttataaca tctaaatgag acttgataaa tttttagtag aatgcggact tggttcacgt
      121 actcaagtca aattaatatt gaaaaaaaag cagattagtg ttaatggtaa tagcgagaca
      181 tctccaaaag tacaggtaga tgaatataga gatgagatta aatataatgg aactctagtg
      241 tcatatgaaa agtttgttta ttatatgctt cataaaccca aaggtgttat atcagcaacg
      301 gatgatcctt cgcataaaac cgtgttagat ttgctagaca aaactgctcg agataaagct
      361 gttttcccag tggggcgttt agatattgat acaacaggcc ttttgttatt aactaataat
      421 ggggaattag cgcataaaat gttatcacct aaaaaacacg ttgataaatg ctatgaggta
      481 aaaatctcag ggataatgac agaagacgat attttggcat ttgataaagg gattattctg
      541 aaagatttta cttgtttacc agcattatta gaaattgtgg aagtaaatca ggtgaagaag
      601 cagagtcttg ttaaaatcac cattaaggaa ggaaaattcc atcaagttaa acgtatggtt
      661 gctgcgtgtg gtaaggaagt cttagagcta aagcgtctaa ggatgggaaa tctacaatta
      721 gataaacagc tagaaagtgg acaatggaga cggctaacaa taaaagaaat agaaaaatta
      781 gaaaaatata tgcaataata taaaacaaca gttaatattt tagcaatttg ataacactta
      841 gattataatg taaacgtata cttttttaga ttggaagatc aaatgaaacg taaatacttt
      901 attcttaata cggtgacggt tttaacgtta gctgctgcaa tgaatactag cagtatctat
      961 gctaatagta ctgagacaag tgcttcagta gttcctacta caaatactat cgttcaaact
     1021 aatgacagta atcctaccgc aaaatttgta tcagaatcag gacaatctgt aataggtcaa
     1081 gtaaaaccag ataattctgc ggcgcttaca acagttgaca cgcctcatca tatttcagct
     1141 ccagatgctt taaaaacaac tcaatcaagt cctgtcgttg agagtacttc tactaagtta
     1201 actgaagaga cttacaaaca aaaagatggt caagatttag ccaacatggt gagaagtggt
     1261 caagttacta gtgaggaact cgttaatatg gcatacgata ttattgctaa agaaaaccca
     1321 tctttaaatg cagtcattac tactagacgc caagaagcta ttgaagaggc tagaaaactt
     1381 aaagatacca atcagccgtt tttaggtgtt cccttgttag tcaaggggtt agggcacagt
     1441 attaaaggtg gtgaaaccaa taatggcttg atctatgcag atggaaaaat tagcacattt
     1501 gacagtagct atgtcaaaaa atataaagat ttaggattta ttattttagg acaaacgaac
     1561 tttccagagt atgggtggcg taatataaca gattctaaat tatacggtct aacgcataat
     1621 ccttgggatc ttgctcataa tgctggtggc tcttctggtg gaagtgcagc agccattgct
     1681 agcggaatga cgccaattgc tagcggtagt gatgctggtg gttctatccg tattccatct
     1741 tcttggacgg gcttggtagg tttaaaacca acaagaggat tggtgagtaa tgaaaagcca
     1801 gattcgtata gtacagcagt tcattttcca ttaactaagt catctagaga cgcagaaaca
     1861 ttattaactt atctaaagaa aagcgatcaa acgctagtat cagttaatga tttaaaatct
     1921 ttaccaattg cttatacttt gaaatcacca atgggaacag aagttagtca agatgctaaa
     1981 aacgctatta tggacaacgt cacattctta agaaaacaag gattcaaagt aacagagata
     2041 gacttaccaa ttgatggtag agcattaatg cgtgattatt caaccttggc tattggcatg
     2101 ggaggagctt tttcaacaat tgaaaaagac ttaaaaaaac atggttttac taaagaagac
     2161 gttgatccta ttacttgggc agttcatgtt atttatcaaa attcagataa ggctgaactt
     2221 aagaaatcta ttatggaagc ccaaaaacat atggatgatt atcgtaaggc aatggagaag
     2281 cttcacaagc aatttcctat tttcttatcg ccaacgaccg caagtttagc ccctctaaat
     2341 acagatccat atgtaacaga ggaagataaa agagcgattt ataatatgga aaacttgagc
     2401 caagaagaaa gaattgctct ctttaatcgc cagtgggagc ctatgttgcg tagaacacct
     2461 tttacacaaa ttgctaatat gacaggactc ccagctatca gtatcccgac ttacttatct
     2521 gagtctggtt tacccatagg gacgatgtta atggcaggtg caaactatga tatggtatta
     2581 attaaatttg caactttctt tgaaaaacat catggtttta atgttaaatg gcaaagaata
     2641 atagataaag aagtgaaacc atctactggc ctaatacagc ctactaactc cctctttaaa
     2701 gctcattcat cattagtaaa tttagaagaa aattcacaag ttactcaagt atctatctct
     2761 aaaaaatgga tgaaatcgtc tgttaaaaat aaaccatccg taatggcata tcaaaaagca
     2821 cttcctaaaa caggtgatac agaatcaagc ctatctccag ttttagtagt aaccctttta
     2881 ttagcttgtt ttagctttgt aacaaaaaag aatcagaaaa gttgattgta atgtaaaata
     2941 attcctattt ttcacttttt agtatcatat tttcgatttt tatgttgtaa taaattataa
     3001 tgaagatgta aggaattata aaataggaaa taatatgaaa gtgaaaaata agattttaac
     3061 gatggtagca cttactgtct taacatgtgc tacttattca tcaatcggtt atgctgatac
     3121 aagtgataag aatactgaca cgagtgtcgt gactacgacc ttatctgagg agaaaagatc
     3181 agatgaacta gaccagtcta gtactggttc ttcttctgaa aatgaatcga gttcatcaag
     3241 tgaaccagaa acaaatccgt caactaatcc acctacaaca gaaccatcgc aaccctcacc
     3301 tagtgaagag aacaagcctg atggtagaac gaagacagaa attggcaata ataaggatat
     3361 ttctagtgga acaaaagtat taatttcaga agatagtatt aagaatttta gtaaagcaag
     3421 tagtgatcaa gaagaagtgg atcgcgatga atcatcatct tcaaaagcaa atgatgggaa
     3481 aaaaggccac agtaagccta aaaaggaact tcctaaaaca ggagatagcc actcagatac
     3541 tgtaatagca tctacgggag ggattattct gttatcatta agtttttaca ataagaaaat
     3601 gaaactttat taaatataaa aaggaggact aacctccttt ttatatttaa atagcccatt
     3661 cgccatttct aaagattggg acagcagttc catctgcacg aataccatca atatccattt
     3721 gttctgaacc aatcataaaa tcaacatgtg ttgaagaacg atttaaacca gcttcatcca
     3781 attcttgact tgtcatttct gttcctcctt ctacattgaa ggcatatgca gttccaatag
     3841 cgaggtgatt agaggcattt tcatcgaata aagtattaaa gaaaatcagt ccagatagtg
     3901 aaattggtgt tttatgtggg acaagtgcaa cttccccaag cgatcttgcc ccatcatttt
     3961 cctcgattaa gcgttggact gtttcttgcc ctttttctgc agtgacattg ataattttac
     4021 cgtctttaaa ggtaaatgtc atattttcga taataacgcc agcataactg agaggttttg
     4081 tacttgtcac atacccatct gcacgacggt aatcaggtgc tgaaaagatt tcttcagtag
     4141 gcatattagc gataaaagtc tctccctgag cgttgagact accggctgcc tcccaaatat
     4201 gatttttagg cataccaagc gttaaatctg tacctggtgc ggtataatga agagcatcaa
     4261 attgttcttg atttagtcta gtagcttttt ctaataattt agcttcatgt gcgtcccaag
     4321 cagcaatagg atcatcttca taaatacgat ttaatttgaa aatggtatcc caaagagcat
     4381 caacttgttg gtcgcttgtt tttaattctg ggaagacaag tgcagcccat tctctaccag
     4441 cagctgcagc cacgttccaa gatactttat tagcttgtat tgctctactt tgttcctcta
     4501 aggcaatagc ggttgctcta gttgtttcag ataaacgttt aggatcaaca gtagcgaaag
     4561 cgttagggtt agaagatttc acaactaatc gactagcctt cttatttaat agtgctaaag
     4621 atttatcaac ggtatactgt ggaacattcg tgagaacttc gtctgaagca tgaagtagtc
     4681 gctggcgcgt gataaaatca tctgtataat caacaataac ttcacttgct cccttttcat
     4741 aagcggcttc agttaaaagc cttgctaagt ggacttgttc cacatcgatt gttaaagcga
     4801 gagtgtgccc tttttggaca tttaaacctt tagaaataat taattgggca tattttttta
     4861 aaaggttgtc gaaatcttgt aaaaccatac tagctccttt aaatttacta ttttttataa
     4921 tttatcataa actcttataa agcacaagga aatatggaag cgatttaata aagatatatt
     4981 gatttaataa agatatattt aagctaacgg tttgtagttt gcgaaataag acgtttcgtg
     5041 ttaatataga cttaactata tattcagtaa atgaggtgac atgagtggct tttggagata
     5101 acggacaacg aaaaaaaact gggtttgaaa aactaactct ttttgttgtt attttgatgg
     5161 tactagtaac agttggtggt ttggtatttg gtgctattag tgcaatcatg taatgagaaa
     5221 cgctgtaaag cgttttttta tactgaatag aataaagaat aggaatttta gaaatgagta
     5281 tgtttttaga tactgccaaa attagtgtta aggcaggtcg cggtggtgat ggcatggttg
     5341 cttttcgccg tgagaaatat gtccccaatg gaggcccgtg gggcggtgat ggcggtaaag
     5401 gcggatcggt catttttaaa gtaaacgaag gtttacgaac tttgatggat tttcgatata
     5461 accgtaattt taaagcaaaa gctggtgaaa aagggatgac taaaggtatg cacggtcgag
     5521 gtgcagaaga ccttattgtg tcattaccac ctggaacaac tgttcgtgat gctactacag
     5581 gtaaggttat taccgacctt gtggagcatg atcaggaatt cgttgtagcc agaggtggcc
     5641 gcggtggtcg tggaaatatt cgctttgcaa ccccacgtaa tccagctcct gaaattgctg
     5701 aaaatgggga gcctggtgaa gaacgtgaat tacaattaga attaaaaatt ttggctgatg
     5761 ttggtctcgt tggtttccca tcagttggga agtcgacgct acttagtgtt gtttcagcag
     5821 caaagcctaa aattggtgcc tatcatttta cgacaattgt ccctaattta gggatggttc
     5881 gtacaaaatc aggtgatagc tttgcaatgg ctgatcttcc tggtttgatt gaaggggcta
     5941 gtcaaggtgt cggattagga actcagtttc ttagacatat tgagcgtaca cgtgttattc
     6001 tacatgttat tgatatgtcg gcaagtgaag gacgagaccc ttacgatgac tatgtttcaa
     6061 ttaataatga gttggaaact tacaatcttc gattgatgga acgtccgcaa attattgttg
     6121 ccaataaaat ggacatgcct gattcagaag agaatttggc agcattcaaa gaaaaattag
     6181 cagcaaacta tgatgagttt gatgacatgc ctatgatttt tccaatttca agtttagcac
     6241 atcaaggact agaaaacctt atggatgcga cggctgaatt attagccaat acagaagagt
     6301 ttcttcttta tgacgaaact gacatgcaag aagatgaagc atactatgga tttaacgaag
     6361 atgaacgtcc atttgagatt acacgtgatg atgatgcaac gtgggtattg tatggtgata
     6421 agctagaaaa actctttgtt atgacaaata tggaacgcga tgaatcaata atgaaatttg
     6481 ctcgtcagtt acgtggtatg ggcgttgatg aagcccttcg agagcgcgga gctaaggatg
     6541 gagatattgt tcgtataggt aacttcgaat ttgaatttgt agattaatgc agtacttgat
     6601 tggaggtttt ttatgggaga taaaccgata tcatttcgtg ataaagatgg gaattttgtt
     6661 tcagcggccg atgtctggaa tgctgaaaaa ttagaagaac tctttaacac attaaatcct
     6721 aatcgtaaac ttcgtttaga acgcgaaaaa ttagcaaaag aaaaaggata gttaagtaac
     6781 tatccttttt agttattttg gtatgaaaaa agactctaaa acataattga tagagtgtta
     6841 aggtacagat tactttatta ttttaaacgt ttttgagctt tttgatatac tgaagagcat
     6901 tttttacaat ggttggagta taagtaataa atttataagc aaaataaaaa gcacaaacta
     6961 aagttataaa aaatagtaag taaataatag ggttttgcgt taagaatcct tgaaaaagaa
     7021 gtacaaggtt aagaattacg gtagagatac ttaagataaa aataactaaa taagcaataa
     7081 tagttgtcca aaaccttaaa tggatgttac tcaccttttc ccatttaata tctaaaaagt
     7141 catctataaa gttgacatga taagtcatat gttctttaat aaagtgcaat attc
//
LOCUS       DATRMA010000083         6729 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.083, whole genome shotgun sequence.
ACCESSION   DATRMA010000083 DATRMA010000000
VERSION     DATRMA010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6729
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.083"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            11..538
                     /locus_tag="VBC28_001728"
     CDS             11..538
                     /locus_tag="VBC28_001728"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEO6815691.1"
                     /translation="MEKKLLRKEVLITLKSQPQAYKSEVDCKLLEAFIKTKAYQNSCV
                     IATYLSFDYEYNTQLLIKQALCDGKRVLVPKTYPKGKMIFVDYQKDNLRTTPFGLLEP
                     VNDRAVEKASIDLIHVPGLIFNNKGFRIGYGAGYFDRYLSDFEGDTISTIYRCQRQDF
                     VEEKHDVAVKEVLCL"
     gene            529..1206
                     /locus_tag="VBC28_001729"
     CDS             529..1206
                     /locus_tag="VBC28_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEO6815692.1"
                     /translation="MSMKKFAKEYPTTVLLVSLTTLVFLLMQLTYGSQAESSQVIFQF
                     GGIQGDYLKAYPTNLWRLISPIFVHIGWEHFLLNGLALYFVGQMGESIWGSLRFLILY
                     ILSGLMGNIFTLFFTPHVVAAGASTSLFGVFSAIAIAGYFGKNPYLKQVGKSYQVMIL
                     LNLFFNIFTPGVSLAGHVGGLVGGVLVAIFLTKQNGSLLFKTWQSILALMIFIIVSIS
                     LIGLSLV"
     gene            1338..2381
                     /locus_tag="VBC28_001730"
     CDS             1338..2381
                     /locus_tag="VBC28_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019315854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HEO6815693.1"
                     /translation="MNRKIIGLTLLSLSVLTLTACGNRSDKSANKSDIKVAMVTNQGG
                     VDDKSFNQSAWEGLQKWGKKKGLTKGNGFDYFQSSNESDHANNLDTAASSGYNLIFGI
                     GFGLHDTIEKVSENNKDVKYVIVDDIIKGKENVASVTFADNEAAYLAGVAAAKTTKTK
                     TVGFIGGMEGVVVKRFEAGFKAGVKSIDPAIKVAVSYAGSFTDAAKGKTIAATQYATG
                     VDVIYQAAGGTGAGIFSEAKTENETRKESNKVWVIGVDRDQSQEGNYVSKDGKKANFV
                     LASTIKEVGKSLQSVAELTEKKQYPGGKVTVFGLKDSGVDIKEHQLSSEGSVAVKKAK
                     EDIVSGKIQVPMK"
     gene            complement(2472..3371)
                     /gene="galU"
                     /locus_tag="VBC28_001731"
     CDS             complement(2472..3371)
                     /gene="galU"
                     /locus_tag="VBC28_001731"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEO6815694.1"
                     /translation="MKVRKAVIPAAGLGTRFLPATKALAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILVVTGKSKRSIEDHFDSNFELEYNLKEKGKNELLKLVDETTGIRLHFIRQ
                     SHPRGLGDAVLQAKAFVGNEPFVVMLGDDLMDITNNKVIPLTKQLINDFEATHASTIA
                     VMEVPHEDVSAYGVIAPQGEGVNGLYSVNTFVEKPSPEEAPSNLAIIGRYLLTPEIFN
                     ILETQKPGAGNEIQLTDAIDTLNKTQRVFARKFTGDRYDVGDKFGFMKTSIDYALQHP
                     QVKDDLKKYIIDLGKSLEKTSKK"
     gene            complement(3408..4424)
                     /locus_tag="VBC28_001732"
     CDS             complement(3408..4424)
                     /locus_tag="VBC28_001732"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEO6815695.1"
                     /translation="MTKQKIAVLGPGSWGTALAQVLNDNGHEVRLWGNVVEQIEEINT
                     NHTNQRYFKDITLDSKIKAYTNLEEAINNVDSILFVVPTKVTRLVAKQVANLLKHKVV
                     LMHASKGLEPGTHERLSTILEEEISEQYRSDIVVVSGPSHAEEAIVRDITLITAASKD
                     IEAAKYVQKLFSNHYFRLYTNTDVVGVETAGALKNIIAVGAGALHGLGYGDNAKAAII
                     TRGLAEITRLGVQLGADPLTFSGLSGVGDLIVTGTSVHSRNWRAGDALGRGEKLEDIE
                     KNMGMVIEGISTTKVAYEIAQNLNVYMPITEAIYKSIYEGANIKDSILDMMSNEFRSE
                     NEWH"
     gene            4594..4923
                     /gene="rnpA"
                     /locus_tag="VBC28_001733"
     CDS             4594..4923
                     /gene="rnpA"
                     /locus_tag="VBC28_001733"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEO6815696.1"
                     /translation="MKKTYRVKSDKDFQMIFSRGKNVANRKFVIYYLEKEQKHFRVGI
                     SVSKKLGNAVVRNAIKRKIRHVLLSQKTALQDYDFVVIARKGVEELDYQALEKNLIHV
                     LKIAGLI"
     gene            4936..5751
                     /locus_tag="VBC28_001734"
     CDS             4936..5751
                     /locus_tag="VBC28_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEO6815697.1"
                     /translation="MKKKLKTFSLILLTGSLLVACGRGEVSSHSATLWEQIVYAFAKS
                     IQWLSFNHSIGLGIILFTLIIRAIMMPLYNMQMKSSQKMQEIQPRLKELQKKYPGKDP
                     DNRLKLNDEMQSMYKAEGVNPYASVLPLLIQLPVLWALFQALTRVSFLKVGTFLSLEL
                     SQPDPYYILPVLAALFTFLSTWLTNKAAVEKNIALTLMTYVMPFIILVTSFNFASGVV
                     LYWTVSNAFQVFQILLLNNPYKIIKVREEAVRVAHEKEQRVKRAKRKASKKRK"
     gene            5759..6580
                     /locus_tag="VBC28_001735"
     CDS             5759..6580
                     /locus_tag="VBC28_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein jag"
                     /protein_id="HEO6815698.1"
                     /translation="MVLFTGATVEEAIEKGLQELNISRLRAHIKVVSREKKGFLGFGK
                     KPAKVEIEGITDEVTDINESVALKNIKNVPSSVDVVEEYIEEVDETLEKEDVSQPELP
                     KIDDKNVVTTSEAIEKIDLLPNIEVAAAQVTKYVENIIYEMDLDATIETTTSKRQINL
                     QIETPEAGRIIGYHGKVLKSLQLLAQNYLHDRFSKSFSVSINVHDYVEHRTETLIDFS
                     KKIARRVLETNEPYHMDPMSNSERKTVHKTIATIEGVESYSEGNDPNRFVVVTKK"
BASE COUNT         2254 a         1042 c         1278 g         2155 t
ORIGIN      
        1 tcatagatat atggaaaaga aattattaag aaaagaagtt ttgataacct taaaatctca
       61 acctcaagct tacaaatcag aagttgactg taaactttta gaggctttta taaaaactaa
      121 agcttatcaa aatagttgtg taattgctac ttacctatct tttgactacg aatacaatac
      181 acaacttctt attaaacaag ctttatgtga tggcaaacgt gtccttgttc ccaaaactta
      241 tccaaaaggt aaaatgattt ttgtggacta tcaaaaagat aacttgagga caacaccgtt
      301 tggcttatta gaacctgtta atgatagagc tgttgaaaaa gctagtatag acttaattca
      361 tgtgcccgga cttatcttta acaataaagg ttttcgtata ggttatggtg caggttattt
      421 tgatcgctat ttgagtgatt ttgaaggtga tactattagt actatttata ggtgtcagag
      481 acaggatttt gtagaagaga agcatgacgt agcagttaag gaggtattat gtctatgaag
      541 aagttcgcta aagagtatcc gacgacagta ctcttggtca gtctgacaac ccttgttttt
      601 ttattgatgc aactaactta tggaagtcaa gcggagtcta gccaagttat ttttcaattt
      661 ggtggaatac aaggtgatta tctaaaagca tatccaacaa atctatggag actaataagt
      721 cctatctttg ttcatattgg ttgggaacat tttttattaa atggtttagc tctgtatttc
      781 gtaggacaaa tgggggaaag tatttgggga agtttgagat ttctaattct ctatatttta
      841 tctggcttaa tgggtaatat ttttacgcta ttttttactc cacatgtggt ggcagcagga
      901 gcctcaacgt ctttatttgg tgttttttct gctatagcaa tcgcagggta ttttggaaaa
      961 aatccttatt taaaacaagt tggaaaatca tatcaagtga tgatcttgtt aaatttattt
     1021 tttaatatct tcacacccgg agtgagttta gcaggccatg ttggtggttt agttggtgga
     1081 gtattggtgg ctattttttt gacaaaacaa aatggttctt tattatttaa aacttggcaa
     1141 agtattttag ctttaatgat atttattatt gtatcgatca gcttaatagg tctatcgttg
     1201 gtataattct acggctatag cctctaaaac caaactttct ctgggaaaaa tagaataaag
     1261 tttgctaaat gattttttta tgttatgata gaaatctgtt tttatgtgac tataacttat
     1321 taggaggttc attctcggtg aacagaaaaa ttattggctt aactctacta tctttatctg
     1381 tactaacatt aacagcatgt ggtaatcgtt cagataagtc tgcaaataaa tcagatatca
     1441 aagttgcgat ggtaactaat caaggtggag tagacgataa atcgtttaat caatcagctt
     1501 gggaagggct acaaaagtgg ggaaaaaaga aggggttgac aaaaggtaat ggctttgatt
     1561 atttccagtc atctaatgaa tcagatcatg ctaataacct tgatacggca gcctctagtg
     1621 gttataattt aatttttggt attggatttg gtcttcatga tactattgaa aaagtatcag
     1681 aaaataataa agatgttaaa tacgttattg tagatgatat cattaaaggt aaagaaaatg
     1741 tagctagtgt tacttttgca gacaatgaag ctgcttactt agctggagtt gctgcagcta
     1801 agacaactaa gacaaaaaca gttggtttta tcggagggat ggaaggcgtc gttgttaagc
     1861 gatttgaagc aggatttaag gctggagtaa aatcaattga tccggctatt aaagttgcgg
     1921 tatcttatgc aggctcattt acagatgcag ctaaaggtaa aacgattgct gccacacagt
     1981 atgcaacagg tgttgatgtt atttaccagg ctgctggtgg tacaggagca ggaattttta
     2041 gtgaggctaa aactgaaaat gagacccgca aggaatctaa caaagtctgg gtaattggtg
     2101 ttgacagaga tcaaagtcaa gaaggtaatt atgtttcaaa agatggtaag aaagcaaact
     2161 ttgttctggc atctactata aaagaggtgg ggaaatctct ccaatctgta gcagaattaa
     2221 cagagaaaaa acaatatcca ggagggaaag taacagtttt tggtctaaag gatagtggtg
     2281 tagatattaa agagcatcaa ttgtctagtg agggatctgt tgctgtcaaa aaagctaaag
     2341 aggatattgt ttcagggaaa atacaagttc ctatgaaatg ataaaagtgt ggaggaacaa
     2401 taagttcttc gtcaagtaca ataaaaaagt ttcctattta ggaaactttt ttattgtact
     2461 tgactcatat tttacttctt agatgttttt tctagagatt ttcctaaatc aatgatatat
     2521 ttcttcaagt catctttaac ttgtgggtgt tgaagagcat aatcaataga tgttttcata
     2581 aaaccaaact tgtcaccaac gtcgtaacgg tctcctgtaa attttctagc aaaaacacgt
     2641 tgcgttttat ttaaagtatc gatagcatcg gttagttgaa tctcatttcc tgcaccaggt
     2701 ttttgagttt ctaaaatatt aaaaatttct ggagttaaca agtagcgacc aatgattgcc
     2761 aaattacttg gagcctcttc gggagaaggt ttttcaacga aagtgttaac gctatataga
     2821 ccattaactc cttctccttg tggagcaata acaccataag cagaaacatc ttcatgtgga
     2881 acttccataa cagcaattgt tgatgcatga gtagcttcaa agtcattaat taattgtttg
     2941 gtgagaggaa taactttatt attggtaata tccattagat catcacctag cataacaaca
     3001 aaaggttcat taccaacgaa ggcttttgct tgtagcacag catctccaag cccacgagga
     3061 tgactttgtc gaataaaatg gagtcgaatg ccagttgttt catcaacaag tttgagaagt
     3121 tcgtttttcc ctttctcttt taggttatat tctaattcaa aatttgaatc aaagtggtct
     3181 tctatagaac gttttgattt accagttaca actaaaatat cttcaattcc agattttagt
     3241 gcttcttcaa caataaattg aatagttggt ttatcaacga ttggaagcat ttctttagct
     3301 aaagctttag tagcaggtaa aaaacgtgtt cctaaaccag cggcagggat aactgctttt
     3361 ctgactttca tatatagctc ctttaaatag tattaatata aacgatatta gtgccattca
     3421 ttctctgaac gaaattcatt ggacatcata tctaaaatgc tatctttgat attagcccct
     3481 tcatatattg atttataaat agcttctgta atgggcatgt aaacatttaa gttttgggcg
     3541 atttcgtaag caactttagt tgttgagata ccttcaataa ccatccccat atttttttca
     3601 atatcttcaa gtttctcacc acgtcctaaa gcatctcccg ctctccagtt gcgagaatga
     3661 acagatgtac cagtcacaat taaatctcca actccggata aaccactgaa agtgagaggg
     3721 tcggccccta attgtacacc aagacgtgtt atctcagcta aaccacgcgt aataatagct
     3781 gctttagcat tatcaccata accaagaccg tgtagtgctc cagcaccaac agcaataata
     3841 ttttttagag caccagctgt ttctactcct acgacatcag tgttagtata aagtctaaaa
     3901 taatgattac taaacaattt ttggacatac ttagctgctt caatatcttt tgaagcagca
     3961 gtaatcaaag taatatcacg aacgatggct tcttcagcat gactgggacc tgaaacaaca
     4021 acgatatcac tacgatattg ttcagagatt tcttcttcta aaattgtaga aagtcgttca
     4081 tgagtaccag gttctagtcc cttagaagca tgcataagaa caactttatg ctttaaaaga
     4141 ttagccactt gtttagcaac aagtcttgta actttagttg ggacgacgaa taaaatgctg
     4201 tcaacattat taatcgcttc ctcaagatta gtgtaggctt ttattttgga atctaaggta
     4261 atatctttga agtaacgttg attagtatgg ttagtattaa tctcttctat ttgttcaacc
     4321 acgtttcccc ataagcgtac ctcatgtcca ttgtcattta atacttgtgc aagagcagtc
     4381 ccccatgaac caggtccaag aacggcaatt ttttgttttg tcatcttatt ccttctttct
     4441 aataataagg catatagcgc taacattata tcatactcta atacactttg tcttgtttag
     4501 tctgttgtca atgatagtaa cattagaaaa gaattgagtc ttttaggtta tttatggtat
     4561 aataaaacca atttagataa atggagcact attttgaaaa aaacctatcg ggtaaaaagt
     4621 gataaggatt ttcaaatgat tttcagcaga ggtaaaaatg ttgctaatcg aaaatttgtt
     4681 atctactatt tagaaaaaga acaaaaacac tttcgtgtgg gaatctctgt tagtaagaaa
     4741 ttaggaaatg ctgttgttcg taacgctatt aaaagaaaaa ttaggcatgt tttactctct
     4801 cagaaaacag cgctgcaaga ttatgatttt gtcgtgattg cgcgtaaagg tgtagaggaa
     4861 ttggactatc aagcattaga aaaaaatctt atacacgttt taaaaatagc aggactcatc
     4921 tagaaaggta taaaattgaa gaaaaaactt aaaacattta gcttaatatt gctgacaggc
     4981 tctttattgg ttgcttgcgg acgaggagaa gtatctagtc attcagccac attatgggag
     5041 caaatagttt atgcatttgc taaatcaata caatggctct cctttaacca ttcaattggt
     5101 ttgggaataa tcttattcac tctaataatc agagcgatta tgatgccgct ttataatatg
     5161 caaatgaaat ccagccaaaa gatgcaggaa attcagccaa ggctcaagga attacaaaaa
     5221 aaatatccag ggaaagatcc tgataaccgt ttaaagctaa atgatgaaat gcaatctatg
     5281 tataaagctg agggagttaa cccatatgca agtgtattac ctcttctaat tcagttacct
     5341 gtcctatggg ctcttttcca agctttgact agggtttctt tcttaaaagt aggaacattt
     5401 ttgagcctag aattatcaca gccggaccca tattatattt tacctgtact ggcggcctta
     5461 tttacctttt tatcaacttg gttaactaac aaagcagctg ttgaaaaaaa tattgcttta
     5521 actcttatga cttatgttat gccgtttatc atattggtaa caagctttaa ctttgctagt
     5581 ggtgttgttt tatactggac ggtgtctaat gcttttcaag tatttcaaat tctattattg
     5641 aacaacccat acaagattat aaaggttcga gaagaagcag taagagtggc acatgaaaaa
     5701 gaacaaagag ttaaacgtgc taagagaaaa gcaagtaaga aaagaaagta ggaaaaccat
     5761 ggtattattt acaggagcga ctgtagaaga agctattgaa aaaggactac aagagttaaa
     5821 catttcacga ttgagggcac atataaaagt tgtatcacgt gagaaaaaag gttttttggg
     5881 ctttggtaaa aagccagcaa aagttgaaat agaaggtatc accgacgaag taactgatat
     5941 taatgaatcg gtagcgttaa aaaatattaa gaatgtaccg tctagcgtag atgtggtgga
     6001 agaatatata gaggaggttg atgaaacgct tgaaaaagaa gacgtatcac aaccagaact
     6061 tccaaaaatt gatgataaaa acgttgtaac tacaagtgaa gctattgaaa aaattgacct
     6121 acttcctaat attgaagtag cagctgctca ggtgactaaa tacgttgaaa atatcatcta
     6181 tgagatggat ttagatgcaa caatagaaac tacgacaagt aagcgacaaa taaatctcca
     6241 aatagaaacg ccagaagctg ggcgcatcat tggttatcat ggaaaagtat taaaatcgtt
     6301 gcagttgtta gcacaaaatt atcttcatga ccgcttttct aagagttttt cagtatccat
     6361 taatgtccat gactacgttg agcatcgtac agaaacttta attgattttt ctaaaaaaat
     6421 agctaggcgt gttttagaga ctaatgaacc ttaccatatg gatcctatga gtaacagtga
     6481 gcgaaaaact gttcataaaa caattgcaac tatagagggt gttgaaagtt attctgaagg
     6541 taacgatcct aatagatttg tagtagttac taagaaataa aaagaggtga gacctctttt
     6601 tttcttgcgc tctatctaca ttttcagaaa atgtagaaaa agtatgaaaa aagagttgac
     6661 aaagtaagag atgatgtagt aaactaaata agttgtctcg agagagcagt ttctattagc
     6721 gcgtagtta
//
LOCUS       DATRMA010000084         6571 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.084, whole genome shotgun sequence.
ACCESSION   DATRMA010000084 DATRMA010000000
VERSION     DATRMA010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6571
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.084"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            <1..282
                     /locus_tag="VBC28_001736"
     CDS             <1..282
                     /locus_tag="VBC28_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6815699.1"
                     /translation="EILKDYILQSLSTDKIRFHSYYLLTDNISQISTLKPDLIITNRK
                     LFTYVTKELATNSLVAHIDYNDTPSQISNIQTIISNIKEEKYRKIFDKI"
     gene            complement(379..2370)
                     /gene="uvrB"
                     /locus_tag="VBC28_001737"
     CDS             complement(379..2370)
                     /gene="uvrB"
                     /locus_tag="VBC28_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HEO6815700.1"
                     /translation="MIDRKDTNRFKLVSKYSPSGDQPQAIETLVDNIEGGEKAQILKG
                     ATGTGKTYTMSQVIAQVNKPTLVIAHNKTLAGQLYGEFKEFFPDNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVVASVSCIYGLGSPKEYA
                     DSVVSLRPGQEISRDQLLNNLVDIQFERNDIDFQRGKFRVRGDVVEVFPASRDEHAFR
                     IEFFGDEIDRIREIESLTGRVLGEVEHLAIFPATHFMTNDEHMEEAISKIQAEMENQV
                     ELFEKEGKLIEAQRIRQRTEYDIEMLREMGYTNGVENYSRHMDGRSEGEPPFTLLDFF
                     PEDFLIMIDESHMTMGQIKGMYNGDRSRKEMLVNYGFRLPSALDNRPLRREEFESHVH
                     QIVYVSATPGDYEMEQTDTVVEQIIRPTGLLDPEVEVRPSMGQMDDLLGEINLRTEKG
                     ERTFITTLTKRMAEDLTDYLKEMGVKVKYMHSDIKTLERTEIIRDLRLGVFDVLIGIN
                     LLREGIDVPEVSLVAILDADKEGFLRNERGLIQTIGRAARNSNGHVIMYADKITDSMQ
                     RAMDETARRRRLQMDYNEKHGIVPQTIKKEIRDLIAITKSNDSDKPEKVVDYSSLSKK
                     ERQAEIKALQQQMQEAAELLDFELAAQIRDVILELKAID"
     gene            complement(2431..3351)
                     /locus_tag="VBC28_001738"
     CDS             complement(2431..3351)
                     /locus_tag="VBC28_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEO6815701.1"
                     /translation="MNKRILNSQFAVYKNWPSWLLLLFACFMVYLFFFVGLTLGGVGI
                     VLVSSVLYKFSSYTFFEFINLFNNIYGELGTFSFSAFLLLIWVKFVEKRPFSELGFST
                     KFKRTLWSLIKGWSIGFILFSISVITAYILGGLDFHSYDVSKATIFYVVTLLPFWLIQ
                     SGTEELLTRGWLLPLINHRFHLAVAIGVSSTLFGILHLVNAHVTFLSIVSIICSGVLM
                     SLYMIKSGNIWSVAALHGAWNFSQGNLYGIAVSGQKAGASLLHFTVKENAPDWISGGA
                     FGIEGSLISIFVLLAAIIYLLWLIKTEETD"
     gene            3534..5717
                     /locus_tag="VBC28_001739"
     CDS             3534..5717
                     /locus_tag="VBC28_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEO6815702.1"
                     /translation="MKHKLKAFTLALLSIFFVFGGKVSAETVNIVSDTAYAPFEFKDS
                     DQTYKGIDVDIVNEVAKRAGWNVNMTYPGFDAAVNAVQSGQADALMAGTTVTEARKKV
                     FNFSDTYYDTSVILYTKNNNKVTNYKQLKGKVVGVKNGTAAQSFLEENKSKYGYKVKT
                     FDTSDLMNNSLDSGSIYAAMDDQPVVQFAINQGKAYAINMEGEAVGSFAFAVKKGSGH
                     DNLIKEFNTAFAQMKSDGTYNDIMDKWLGKDATKTSGKATGNANEKATPVKPSYKIVS
                     DSSFAPFEYQNGKGKYTGFDMELIKKIAKQQGFKLDISNPGFDAALNAVQSGQADGVI
                     AGATITEARQKIFDFSDPYYTSSVILAVKKGSNVKSYQDLKGKTVGAKNGTASYTWLS
                     DHADKYNYHVKAFDEASTMYDSMNSGSIDALMDDEAVLAYAINQGRKFETPIKGEKSG
                     DIGFAVKKGANPELIKMFNNGLASLKKSGEYDKLVKKYLSTASTSSNDKAAKPVDEST
                     ILGLISNNYKQLLSGIGTTLSLTLISFAIAMVIGIIFGMMSVSPSNTLRTISMIFVDI
                     VRGIPLMIVAAFIFWGIPNLIESITGHQSPINDFVAATIALSLNGGAYIAEIVRGGIE
                     AVPSGQMEASRSLGISYGKTMQKVILPQAVRLMLPNFINQFVISLKDTTIVSAIGLVE
                     LFQTGKIIIARNYQSFRMYAILAIMYLVIITLLTRLAKRLEKRLK"
     gene            5717..6457
                     /locus_tag="VBC28_001740"
     CDS             5717..6457
                     /locus_tag="VBC28_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003107174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEO6815703.1"
                     /translation="MAELKIDVQDLHKSYGQNEVLKGIDAKFYEGDVVCIIGPSGSGK
                     STFLRTLNLLESITSGKVVVDGFELSNPKTDIDKARENIGMVFQHFNLFPHMSVLENI
                     TFAPIELGKESKEAAEKHGMELLEKVGLADKANAKPDSLSGGQKQRVAIARSLAMNPD
                     ILLFDEPTSALDPEMVGDVLNVMKDLAEQGMTMLIVTHEMGFARQVANRVIFTDGGRF
                     LEDGTPEQIFDTPQHPRLQDFLNKVLNV"
BASE COUNT         2208 a         1251 c         1073 g         2039 t
ORIGIN      
        1 gaaattttaa aagactatat tttgcaatcc ctttcaacag ataaaattag atttcattcc
       61 tattacttat tgacagataa tattagtcaa atatcgacct taaaacccga tttaattatc
      121 actaatcgaa aactgtttac ctatgttaca aaggaactag ctacaaatag tttagtcgct
      181 catattgatt acaacgatac accctctcaa atcagtaata tccaaactat tatttctaat
      241 attaaagaag aaaaatatcg aaaaatcttt gataaaatat aacatcacct actgtattct
      301 tttgatttca attattaata aaacgactag agcacttcat taactactct agtcgtttta
      361 ttaattctgt tcatcgtatt aatcgattgc ttttagttct aagataacat ctcggatttg
      421 tgcagctagc tcaaagtcca ataattcagc tgcttcttgc atttgctgtt gaagagcttt
      481 aatttcagct tgcctttctt ttttagataa actactgtaa tcgacaactt tttcaggctt
      541 atcgctgtca ttacttttag taatagcaat taaatcacgg atttcttttt taattgtttg
      601 tggcacaatt ccatgctttt cattataatc catttgtaaa cgacgacggc gagcggtttc
      661 atccatagct ctttgcatag aatctgttat tttatcagca tacataataa catgaccatt
      721 actgttacgt gctgcacgtc cgattgtttg gataagacca cgctcattac gaaggaaacc
      781 ttccttatca gcatctaaga ttgcaacaag ggaaacctca ggaacatcta ttccttcacg
      841 taagagatta ataccaatta aaacatcaaa cacaccaagt cgtaaatcac gaataatctc
      901 tgtacgttct aaggttttaa tatcgctgtg catatactta actttaactc ccatttcttt
      961 gagataatct gttaagtctt ctgccatacg ctttgtcaat gttgtaataa aggtacgctc
     1021 acctttttca gtcctcaaat tgatttcacc aagcaagtca tccatctgcc ccatacttgg
     1081 tctaacttca acttctggat caagaagtcc tgttggccgg ataatttgct caacaactgt
     1141 atctgtttgc tccatttcat agtctcctgg cgttgcagat acatagacaa tttgatggac
     1201 atggctttcg aattcttcac gacgaagtgg acggttatct aaggcagatg gtaaacggaa
     1261 accgtaatta accaacattt ctttacgcga acggtctcca ttatacatcc ctttgatttg
     1321 ccccattgtc atgtgactct catcaatcat aatcaaaaaa tcttctggga agaaatctaa
     1381 caaagtaaaa ggtggctcac cttcacttct accatccatg tgacgtgagt agttctcaac
     1441 accatttgta tatcccattt cacgaagcat ttcaatatca tattctgtcc tttgtcgaat
     1501 acgttgcgct tcaatcaatt tcccttcttt ttcaaaaagt tctacttgat tttccatttc
     1561 agcttgtatt tttgaaatag cttcttccat atgttcatca tttgtcataa agtgtgttgc
     1621 tgggaaaata gctaaatgtt ccacttctcc tagcacacgc cctgttaacg attctatttc
     1681 tcttatacga tcaatttcat caccaaaaaa ttcaattcga aaggcatgct catcacgact
     1741 cgcaggaaaa acttctacaa catcgccacg aacgcgaaat tttccacgtt gaaaatcaat
     1801 atcgttacgc tcaaattgaa tatcaacgag attgttcaat aactgatcac gtgaaatttc
     1861 ttgacctggc ctaaggctaa caacactgtc tgcatactct ttaggagaac ctaaaccgta
     1921 aatacaagaa acagatgcta caacaataac gtcatttctt tctaataacg aagaagtcgc
     1981 tgagtgacgt aatttgtcaa tttcgtcatt gactgaacta tctttttcaa tataagtatc
     2041 cgaagaaggc acataagctt ctggttgata ataatcataa tatgaaacaa agtactctac
     2101 ggcattgtca ggaaaaaatt ctttaaactc tccataaagt tgacctgcta aggttttatt
     2161 atgtgcaatc accaaggttg gcttgttaac ttgggcgata acttggctca ttgtgtaggt
     2221 tttaccagta ccagtagcac cttttaaaat ctgagctttt tctcctcctt caatattgtc
     2281 aaccaacgtt tcaatagctt gaggttgatc tcccgaaggg ctatatttgg aaactaattt
     2341 aaatcggtta gtatcttttc tatctatcat ttctttacct cgattacttt attttaacat
     2401 agaaaaaaga aaaagactga gctgcaacac tcaatctgtt tcttcagtct taattagcca
     2461 tagaaggtat ataattgctg ctaagagaac aaaaatagaa atcaaacttc cttctattcc
     2521 gaatgcacca cctgatatcc agtccggtgc gttttcttta acggtaaaat gcaaaagaga
     2581 tgctcctgct ttctgaccac ttacggcaat accatagaga ttaccttggc taaaattcca
     2641 agctccatgt aaggctgcaa cactccaaat attaccactt ttaatcatat acaaggacat
     2701 taaaactccc gaacatataa tacttacaat agaaagaaaa gtgacatggg catttaccaa
     2761 gtgcaaaatg ccaaataaag tggatgagac ccctatggca acagctaaat gaaaacgatg
     2821 attaattaga ggaagaagcc accctcgtgt tagcaattct tccgtaccgc tttgaataag
     2881 ccaaaaagga aggagagtta caacatagaa aatcgttgcc ttagaaacat catagctatg
     2941 aaagtcaagt cctcccaata tataagcagt gatgacagaa attgaaaata gaataaaacc
     3001 tatactccag ccttttataa gtgaccacag agttctttta aactttgtag aaaaccccaa
     3061 ttctgaaaaa ggtctttttt ctacgaattt tacccagata agcaaaagga atgctgaaaa
     3121 actaaaggtt cctaattctc catagatatt attaaataaa ttaataaact caaaaaaagt
     3181 ataggaagaa aacttataga gtacagaaga cactagaaca attcccacac cacctagagt
     3241 tagaccaacg aaaaaaaata ggtataccat aaaacaagca aataacaata atagccatga
     3301 cggccagttt ttatagacag cgaactgact atttaaaatc cttttattca taataatcct
     3361 aaatcctttt tgtgtgataa cactagaacg attatatcat aatactaact aaagatatat
     3421 ataaaattaa cataacatac atgttacgtt ttctaacata gaaatgacat tttgttgctc
     3481 tatcttattt attttgatat aataaaacac atatttataa aaaggagatt tacatgaagc
     3541 acaagttaaa agcttttacg cttgctttac tctcaatatt ctttgtgttt ggtggaaagg
     3601 tcagtgcaga gactgtgaat attgtttctg atacagcata cgctccattc gaatttaaag
     3661 attctgatca aacttataaa ggaatcgatg ttgacatcgt taacgaagtc gctaagcgtg
     3721 ctggctggaa tgttaacatg acgtacccag gttttgatgc cgcagttaac gctgttcaat
     3781 ctggacaggc agacgcgcta atggccggaa ctactgttac tgaagcacgt aaaaaagtct
     3841 ttaatttctc agatacttat tacgatactt ccgttattct ttatactaaa aataataata
     3901 aagtcacaaa ctacaaacaa ctaaaaggaa aagtagtcgg tgtaaaaaat ggaacagctg
     3961 ctcaaagctt cttagaggaa aataaatcta aatacggcta taaagttaaa acatttgata
     4021 caagtgacct aatgaataac agccttgatt ctggttctat ttacgccgct atggacgatc
     4081 aaccagttgt gcaatttgcg ataaatcaag gaaaagctta cgccattaac atggaaggcg
     4141 aggcagttgg tagctttgca tttgctgtca aaaaaggtag tggacacgat aacctaatta
     4201 aagaatttaa cacagctttt gcacaaatga aatcagatgg cacttataat gacatcatgg
     4261 ataaatggct tgggaaagac gctacaaaaa caagcggcaa agcaacaggt aatgccaatg
     4321 aaaaagcaac tcctgtaaag ccaagttata aaattgtttc tgattcttca ttcgcaccat
     4381 tcgaatatca aaacggtaaa gggaaatata ctggttttga tatggaatta atcaagaaaa
     4441 ttgctaaaca gcaaggtttt aaacttgata tctcaaatcc aggttttgat gccgctttaa
     4501 atgctgtcca atctggacaa gctgacggtg ttattgcagg agctacaatc acagaagcac
     4561 gccaaaaaat ctttgatttt tctgatcctt attacacatc tagcgttatc ttagcggtta
     4621 aaaaaggaag caatgtcaaa tcataccaag atttaaaagg aaaaacagtt ggtgctaaaa
     4681 atggtactgc ctcatatact tggttatcag accacgcaga taagtacaac tatcatgtta
     4741 aagcatttga tgaagcatct acaatgtatg atagtatgaa ctcaggttca attgatgctc
     4801 taatggatga cgaagcagtt cttgcttacg ctattaatca aggtcgtaaa tttgaaacac
     4861 ctatcaaagg tgaaaaatca ggcgatatcg gatttgcagt gaaaaaaggg gcaaatccag
     4921 aattaattaa aatgtttaac aacggtcttg cttcactcaa aaaatcgggt gagtacgata
     4981 aacttgttaa aaaatacctt tccacagcca gcacctcttc aaacgataaa gctgctaaac
     5041 ctgtagatga atcaactatt ttagggttaa tttctaataa ctacaaacaa ttgctatctg
     5101 gtattggaac tactttaagt ttaactctta tctcatttgc gattgctatg gttattggta
     5161 ttatctttgg tatgatgagc gtatcaccaa gtaatactct ccgcacaatt tcaatgattt
     5221 ttgttgatat tgtccgtggt attccactca tgattgtggc cgcttttatt ttctggggta
     5281 ttcctaattt aatcgaaagc atcacaggtc accaaagtcc aattaatgac ttcgttgctg
     5341 ctactatcgc tctttcttta aatggtggtg cgtacattgc tgaaattgta cgtggtggta
     5401 ttgaagctgt tccttctggt caaatggaag caagtcgtag tttaggtatt tcttacggca
     5461 aaactatgca aaaggttatc ttgcctcaag cagtacgcct tatgttacca aactttatca
     5521 accaatttgt catctcatta aaggatacaa caattgtatc agcaatcgga cttgtggaac
     5581 tcttccaaac tggtaaaatc ataattgcac gtaactatca atcattccgt atgtatgcta
     5641 tccttgcaat tatgtatctt gtaatcatta cccttctcac ccgtctagca aaacgtttag
     5701 aaaagaggct taaataatgg cagaattaaa aattgatgtc caagatttac acaagtcgta
     5761 tgggcaaaat gaagttttaa aaggtattga tgctaaattt tatgaaggtg atgttgtttg
     5821 tattattgga ccttctggtt caggaaaatc aactttcctt cgcacattga acttacttga
     5881 atcaatcaca agtggaaaag ttgttgtgga cggctttgag ttatcaaacc caaaaacaga
     5941 catagacaaa gcacgtgaaa atatcggaat ggtatttcaa cacttcaatc ttttcccaca
     6001 tatgtcagtt cttgaaaaca tcacttttgc tcctatagaa cttggtaaag aatctaaaga
     6061 agcagctgaa aagcatggga tggaattact tgaaaaagtt ggtttagccg ataaagctaa
     6121 tgccaaacca gatagtttat ctggaggaca aaaacaacgt gtagctatcg caagaagctt
     6181 agcgatgaat ccagatatct tactttttga cgaaccaact tctgcacttg atccagaaat
     6241 ggttggagat gttctcaatg ttatgaaaga tttagctgaa caaggtatga ctatgttaat
     6301 tgtgacacac gaaatgggat ttgctcgtca agttgccaat cgtgttatct ttacagatgg
     6361 tggtcgtttc ctcgaagatg ggacacctga gcaaatcttt gatacacctc aacacccacg
     6421 tctccaagac ttccttaata aagttttaaa cgtttaataa aaaagatgac ttcgtgtcat
     6481 ctttttttat atcctaatca caaacaaatt ataataggat aagttttctt ctaaaattag
     6541 caaaacatca gctagaatgt ttgaataatg t
//
LOCUS       DATRMA010000085         6550 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.085, whole genome shotgun sequence.
ACCESSION   DATRMA010000085 DATRMA010000000
VERSION     DATRMA010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6550
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.085"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            complement(579..728)
                     /gene="rpmG"
                     /locus_tag="VBC28_001741"
     CDS             complement(579..728)
                     /gene="rpmG"
                     /locus_tag="VBC28_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEO6815704.1"
                     /translation="MRVNITLEHKESGERLYLTSKNKRNTPDRLQLKKYSPKLRKHVV
                     FTEVK"
     gene            complement(744..926)
                     /gene="rpmF"
                     /locus_tag="VBC28_001742"
     CDS             complement(744..926)
                     /gene="rpmF"
                     /locus_tag="VBC28_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEO6815705.1"
                     /translation="MAVPARHTSKAKKNKRRTHYKLTAPSVQFDETTGDYSRSHRVSL
                     KGYYKGRKIAKANEAK"
     gene            1146..2426
                     /locus_tag="VBC28_001743"
     CDS             1146..2426
                     /locus_tag="VBC28_001743"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEO6815706.1"
                     /translation="MKLQKPKGTQDILPGESAKWQYVENVIRNLFKQYHYDEIRTPMF
                     EHYEVISRSVGDTTDIVTKEMYDFHDKGDRHITLRPEGTAPVVRSYVENKLFAPEVQK
                     PTKMYYIGSMFRYERPQAGRLREFHQVGVECFGSNNPATDVETIAMGHHLFEDLGIKN
                     VKLHLNSLGNPESRQAYRQALIDYLTPIREQLSKDSQRRLNENPLRVLDSKEPEDKLA
                     VENAPSILDYLDESSQAHFDAVCHMLDALNIPYIIDTNMVRGLDYYNHTIFEFITEIE
                     DNELTICAGGRYDGLVSYFGGPETPAFGFGLGLERLLLILDKQGISLPIENTIDLYIA
                     VLGSEANLAALDLAQSIRHQGFKVERDYLGRKIKAQFKSADTFNAKVIMTLGSSEVDS
                     KEVGLKNNQTRQEVKVSFENIKTDFSSVLKQLGL"
     gene            2519..4270
                     /gene="aspS"
                     /locus_tag="VBC28_001744"
     CDS             2519..4270
                     /gene="aspS"
                     /locus_tag="VBC28_001744"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEO6815707.1"
                     /translation="MKRSMYAGRVRSEHIGTSITLKGWVGRRRDLGGLIFIDLRDREG
                     IMQLVINPEEVSASVMATAESLRSEFVIEVSGVVTAREQANDNLPTGEVELKVQELSI
                     LNTSKTTPFEIKDGIEANDDTRMRYRYLDLRRPEMLENFKLRAKVTHSIRNYLDNLEF
                     IDVETPMLTKSTPEGARDYLVPSRVNQGHFYALPQSPQITKQLLMNAGFDRYYQIVKC
                     FRDEDLRGDRQPEFTQVDLETSFLSDQEIQDIVEGMIAKVMKDTKGLEVSLPFPRMAY
                     DDAMNNYGSDKPDTRFDMLLQDLTEIVKEVDFKVFSEASVVKAIVVKDKADKYSRKNI
                     DKLTEIAKQYGAKGLAWLKYADNTISGPVAKFLTAIEGRLTEALQLENNDLILFVADS
                     LEVANETLGALRTRIAKELELIDYSKFNFLWVVDWPMFEWSEEEGRYMSAHHPFTLPT
                     AETAHELEGDLAKVRAVAYDIVLNGYELGGGSLRINQKDTQERMFKALGFSAESAQEQ
                     FGFLLEAMDYGFPPHGGLAIGLDRFVMLLAGKDNIREVIAFPKNNKASDPMTQAPSLV
                     SEQQLEELSLTVESYEN"
     gene            4260..5204
                     /locus_tag="VBC28_001745"
     CDS             4260..5204
                     /locus_tag="VBC28_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEO6815708.1"
                     /translation="MKTRRTPLEKKVKYIISVWAKKFGLLHTLKSISREKYAEKISAS
                     LLYGILSSVAVNFFFQPGHVYSSGATGLAQVISAVSKHWFSFEIPVALAFYAINIPLL
                     ILSWRKIGHKFTIFTFITVTVSSIFIQLMPQITLTTDPLINAIFGGLIMGAGVGFSFK
                     SRISSGGTDIISLTIRKKTGRDVGSISFIINGIILLFAGLLFGWKYALYSMVTIFVSS
                     RVTDAIFTKQKKMQAMIVTSKPDCVIKRIHRDLHRGVTCINDAEGTYNHEKKAVLITI
                     LTREEFSDFKYLMLKADPKAFVSVAENVHIIGRFVDDD"
     gene            5312..6184
                     /locus_tag="VBC28_001746"
     CDS             5312..6184
                     /locus_tag="VBC28_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEO6815709.1"
                     /translation="MLKLDLKTKIKETILIAFGVALYTFGFVKFNMANHLAEGGISGV
                     TLIIHALFGVNPALSSLLLNIPLFILGARILGKKSLLLTIYGTVLMSFFMWFWQQIPV
                     TVPLKNDMMLVAVAAGILAGTGSGLVFRYGATTGGADIIGRIVEEKSGIKLGQTLLFI
                     DAIVLTSSLVYINLQQMLYTLVASFVFSQVLTNVENGGYTVRGMIIITKESESAAATI
                     LHEINRGVTFLRGQGAYSGREHDVLYVALNPSEVRDVKEIMADLDPDAFISVINVDEV
                     ISSDFKIRRRNYDK"
     gene            complement(6211..6519)
                     /locus_tag="VBC28_001747"
     CDS             complement(6211..6519)
                     /locus_tag="VBC28_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEO6815710.1"
                     /translation="MPSEKEILDALSKVYSEEVIQADDYFRQAIFELASQLEKEGMNS
                     LLATKIDSLINQYVLTHQFDAPKSIFDLSRLVKTKASHYKGTAISAIMLGSFLSGGPK
                     "
BASE COUNT         2069 a         1016 c         1295 g         2170 t
ORIGIN      
        1 ttaatgatag tgattatgtt ctatactcag gaaataattt cattcctaga tttagtattt
       61 tagggtctat atcgcaatat gggaacgagc tctattactt attatttgga gagcgagagc
      121 ttcacatttt tgaaggagat ttttcgaaat atgtatcagg aagaaagaac ataatgttta
      181 ataagaattt tttaaacctt ttgaaaaaga tacaaagcct atcggagtca gaaaacagaa
      241 tagatatttc taattttaaa aaagactttg aagattcttg gactatattt gtatcaaatg
      301 acgaagttca gctagaaaat ttatttcata aaaatttgtc atattatcga aatgtaatta
      361 aagaaaatta ttcctatttg aatatgctta cagagataaa ttacacaaag acaaatacca
      421 taagttctat cgttgctacg atagtctcca tcattgctac cattatatct gttattgcac
      481 tcctaaagtg atataatcag ttaaaattca gttattttgc taaaatcaaa agaaaaaagc
      541 ctatgaaacg ttgatttcat aggctttttt tattttaatt atttaacttc agtgaaaaca
      601 acgtgtttac gaagttttgg tgagtatttt ttcaattgaa gacggtctgg agtgttacgt
      661 ttgttttttg aagtaaggta caagcgttca ccagattctt tatgttcaag tgtaatattt
      721 acgcgcatgg tatctccctt ctattattta gcttcgttag ctttagcgat tttacgtcct
      781 ttgtagtatc ctttgagtga tacacggtgt gaacgtgagt aatctccagt agtttcgtcg
      841 aattgtacag atggagctgt caatttgtag tgtgtacgac gtttgttttt cttcgctttt
      901 gaagtgtgac gtgcaggtac tgccattttc ttattcttcc tttcgatgtg taagttttga
      961 ttttcatcaa cttcactatg ataacatatc ttttttgtaa agtaaagtta cttgacaaaa
     1021 ctttttattt ttacaatttc acggtgaaaa tactatcaaa ctaaagcaaa tgatatatgc
     1081 ttgtttaaaa tataagaaaa aatggtagaa tgtttaaaga gaaaactaat cgaagaggta
     1141 aacttatgaa acttcaaaaa ccaaaaggga ctcaggatat cttacctgga gaatcagcaa
     1201 agtggcaata cgttgaaaat gttatacgca atctctttaa acaatatcat tatgatgaaa
     1261 ttcgtacacc aatgtttgaa cattacgagg ttattagtcg ctcagttggg gatacaacgg
     1321 atatcgttac aaaagagatg tatgattttc atgataaagg agatcgtcac attacgcttc
     1381 gtccagaagg aacagcacct gttgtacgtt cttatgttga aaataaacta tttgcaccag
     1441 aagttcaaaa accaaccaaa atgtattata ttggatcaat gttccgatat gagcgcccac
     1501 aggctggtcg tttacgagaa tttcatcaag taggcgttga gtgctttgga tcaaataatc
     1561 cagcaactga tgttgaaaca atcgcaatgg gacatcatct atttgaagat ttaggtatca
     1621 aaaacgttaa acttcatctt aatagtttag ggaatccaga aagtcgtcag gcctatcgcc
     1681 aagctcttat tgattactta acacccatac gtgaacaatt gtctaaggat agtcaacgtc
     1741 gtttgaatga aaatccattg cgcgtactag actctaagga gccagaagac aaactagcag
     1801 ttgaaaatgc tccttctatt ttagattatt tagatgagtc aagccaagcc cactttgatg
     1861 ctgtgtgtca tatgcttgat gctctaaata ttccatacat cattgatact aatatggttc
     1921 gtggtcttga ttattataat catactattt ttgagtttat cactgagatt gaagataatg
     1981 aattaacgat ctgtgctgga ggccgttatg acggcttagt atcctatttt ggtggaccag
     2041 aaacaccagc atttggtttt ggattaggat tagaacgctt gctcttaata ttagataagc
     2101 aagggatttc tttaccaata gaaaatacta tagatctgta tatagctgta ttaggatcag
     2161 aggctaatct agctgcctta gatttggcac aatcaatacg tcatcagggt tttaaagtag
     2221 agcgtgatta tttgggtcgt aaaatcaaag cacaatttaa gtctgcagat acttttaatg
     2281 ctaaagtgat tatgacatta ggtagcagtg aagtagattc gaaagaggtc ggtttaaaaa
     2341 ataatcaaac acgtcaagaa gttaaagtta gttttgaaaa cattaaaaca gatttttcat
     2401 ctgtgcttaa acaattagga ttgtaaaaca gttcctaaaa actctcctta atgggtgggg
     2461 tttttaggtg ctttttgtta taataggttg gaatgaatat tttaaatgga gaaaacagat
     2521 gaaacgtagt atgtatgctg ggcgtgttcg tagtgagcac ataggaacat cgattacttt
     2581 aaaaggatgg gttggtcgtc gtcgtgacct tggaggcctt atttttattg atcttcgcga
     2641 tcgcgaggga atcatgcaat tagttattaa tcctgaagaa gtatcagcct cagttatggc
     2701 aaccgcagaa agtctccgta gtgaatttgt tatagaggtt agcggtgtag tgacagcacg
     2761 agaacaagct aatgataatt taccaacagg agaagttgaa ttaaaagtgc aagaattatc
     2821 gattcttaat acctcgaaaa caacaccatt tgaaattaaa gatggtattg aggcaaatga
     2881 tgatacacgc atgcgttacc gttacttgga tcttcgtcgt ccggaaatgc ttgaaaattt
     2941 caaattacgt gcgaaagtaa cacactcaat ccgtaattac cttgacaact tagagtttat
     3001 tgatgtcgaa actccgatgc taacaaaatc aactccagag ggggcacgag actatttggt
     3061 tccatcacgt gtcaaccaag gacattttta tgcccttcca caaagccctc aaattacaaa
     3121 acagctattg atgaatgcgg gatttgatcg ttattatcaa attgtcaaat gtttccgtga
     3181 tgaggattta cgtggagacc gtcaacctga gtttacacaa gttgacttag agacatcatt
     3241 ccttagtgat caagaaattc aagacattgt tgagggtatg attgctaaag taatgaaaga
     3301 cactaaaggt ttagaggttt ctttaccatt cccaaggatg gcatatgatg atgcaatgaa
     3361 taactacggg tctgataagc ctgatacaag gtttgatatg cttttacaag acttgacaga
     3421 aattgtcaaa gaagttgact tcaaggtgtt ctcagaagct tcagtagtta aagcgattgt
     3481 tgtcaaagat aaggcggaca agtactctcg aaaaaatatt gataaattaa cagaaattgc
     3541 aaaacaatac ggtgccaaag gtttagcatg gctaaaatat gcagataata ctatttcagg
     3601 tccggttgct aagttcttga cagctattga aggtaggttg acagaagctt tacagctaga
     3661 gaataatgac ttaattctat ttgtagcaga tagtcttgaa gtagctaacg agactttagg
     3721 agcgcttcgt acacgcattg caaaagaact tgaactcatt gattattcta aatttaattt
     3781 cctatgggtt gttgattggc caatgtttga gtggtcagaa gaagagggac gatatatgtc
     3841 ggctcatcat ccattcacct tgccaacagc ggagacagcg catgagttgg aaggggattt
     3901 ggctaaagta cgtgcagttg cctatgatat tgttttgaat ggatatgagt taggcggtgg
     3961 tagcctacgt attaaccaaa aagatacaca agagcgtatg ttcaaggctt taggatttag
     4021 cgctgaatca gctcaggaac agtttggttt cttacttgaa gctatggatt acggcttccc
     4081 accacatggt ggattggcaa taggccttga ccgctttgtt atgttgttag caggcaagga
     4141 caatattcga gaagttattg cattccctaa aaacaataag gctagtgatc caatgactca
     4201 agctcctagt ctcgtttcag aacaacaatt agaagaatta tcacttactg tagaaagtta
     4261 tgaaaactag acgaacccct ttagaaaaaa aggtaaaata tattatcagt gtatgggcta
     4321 aaaaatttgg tctcttacac actttaaaaa gtatctcacg agaaaaatat gcagagaaaa
     4381 tttcggcttc attgctgtat ggtattctat ccagtgttgc tgtcaatttc ttttttcaac
     4441 ctggacatgt ttattcaagt ggagcaactg gtttagctca ggttatttca gctgtcagta
     4501 aacactggtt ttcttttgaa ataccagttg ctttagcttt ttatgccatt aatatccctt
     4561 tgctgatatt atcttggcgt aagattggac ataaatttac tatttttacg tttatcacag
     4621 taactgtaag ctctatcttc attcaactaa tgcctcaaat cacccttaca actgatccct
     4681 taatcaatgc tatctttggt ggtttaatta tgggagctgg tgttggtttt agctttaagt
     4741 ctagaatttc tagtggggga acagatatta tcagtttgac aattagaaag aaaacaggca
     4801 gagatgtagg tagtatttct tttattatta atggtattat tttattattt gcaggtcttt
     4861 tatttggttg gaagtacgct ttgtactcga tggtgaccat ttttgtatca agtagagtta
     4921 cagatgccat ttttacaaag caaaaaaaga tgcaagcgat gattgttacc agtaagcctg
     4981 attgcgtcat taaacgtatc catcgtgatt tacatcgtgg tgtaacctgt atcaatgatg
     5041 ctgaaggtac ttataatcat gagaaaaaag cagttttaat aactatatta acacgtgaag
     5101 aatttagtga ttttaaatat ttaatgctaa aagcggatcc aaaagccttt gtttccgttg
     5161 cagaaaatgt tcatattatt ggacgattcg ttgatgatga ttaactgtat atatgacttt
     5221 tggtcagcca ccactaagga aacattatga tgttattaag ctaagttgtt taaacaactt
     5281 tagctttttt gaataaggaa ggattaagcc tatgttgaaa ctggatttaa aaacaaagat
     5341 aaaagaaact attttaattg cctttggtgt ggctctctat actttcggct ttgtcaaatt
     5401 taatatggct aatcatttgg ctgaaggagg tatttccggt gtcactttga ttatccatgc
     5461 acttttcgga gttaatccag cactttcatc acttctcctt aatatcccgt tatttatatt
     5521 aggggctagg attttaggga aaaaatcctt attgttaact atatatggta cagtgttaat
     5581 gtcttttttc atgtggtttt ggcaacaaat ccctgtaaca gtccctttga aaaacgatat
     5641 gatgttagtt gcagtagctg ctggaatttt agcaggaact ggtagtggtt tagtatttag
     5701 atatggtgca actacaggtg gtgcagatat tattggtcgt attgtagagg aaaaatctgg
     5761 aataaaatta gggcaaacct tattgtttat tgatgctata gttctaacgt cttcacttgt
     5821 ttatattaat ttacaacaga tgctatatac gttagtagct agctttgtat ttagtcaagt
     5881 gttaacaaat gttgaaaatg ggggctacac tgttcgtgga atgatcatta ttaccaaaga
     5941 atcagaatct gcagcagcaa ccattttgca tgaaattaat aggggtgtaa cttttttacg
     6001 gggccaaggt gcctactcag gtagagagca tgatgtcttg tacgtagctc ttaacccaag
     6061 tgaagtgcgt gatgtgaaag aaatcatggc tgatttagac ccagatgctt ttatttctgt
     6121 tataaatgta gatgaagtta ttagctcaga ttttaaaatt cgacgaagaa attacgataa
     6181 ataaaaatac aggctgtctg gcctgtattt ttattttggt cctccactta agaaactacc
     6241 aagcataata gctgagatag cagttccctt gtaatggcta gcttttgttt ttaccaagcg
     6301 actaagatca aatatgcttt taggagcatc aaattgatgt gttaagacgt attgattgat
     6361 aagtgagtca atttttgtag ctaggagtga gttcatcccc tctttttcaa gttgactagc
     6421 gagctcgaaa atagcttgac gaaaataatc atctgcttgt attacttctt cgctataaac
     6481 tttactaaga gcatctaata tttctttttc tgatggcatg gcattctcct gtaacttgtt
     6541 gtgctattat
//
LOCUS       DATRMA010000086         6463 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.086, whole genome shotgun sequence.
ACCESSION   DATRMA010000086 DATRMA010000000
VERSION     DATRMA010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6463
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.086"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            33..905
                     /locus_tag="VBC28_001748"
     CDS             33..905
                     /locus_tag="VBC28_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEO6815711.1"
                     /translation="MSKKIKNFILLNIGSLIVAFGFNAFFLHNHIVTGGISGLAVSFH
                     EVFGWDSAFFIMIINIPLLLLCYFGLGKQTFLKTVYGSWIFPVFIKLTQSVPTLTHNP
                     LLAALFGGVIVGCGLGIVFWSDSSTGGTGIIIQFLGKYTPISLGQGVILIDGLVTIVG
                     FLAFDSDTVMFSIIGLITISYIINAIQTGFTTLSTVLIVSQEHQKIKTYINTVADRGV
                     TEIPVKGGYSGTNQIMLMTTIAGYEFAKLQEAIAEIDETAFITVTPTSQASGRGFSLQ
                     KNHGRLDEDILMPM"
     gene            971..2590
                     /locus_tag="VBC28_001749"
     CDS             971..2590
                     /locus_tag="VBC28_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO6815712.1"
                     /translation="MLTVSDVSLRFSDRKLFDEVNINFTAGNTYGLIGANGAGKSTFL
                     KILAGDIEPTTGHIALGPDERLSVLRQNHFDYEDERVIDVVIMGNETLYSIMKEKDAI
                     YMKEDFSDEDGVRAAELEGEFAELGGWEAESEASQLLQNLNISEELHYQNMSELANGD
                     KVKVLLAKALFGKPDVLLLDEPTNGLDIQSITWLEDFLIDFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLFVGNYDFWKESSELAARLQADRNAKAEEKIKQLQEFVARFSANA
                     SKSKQATSRKKMLDKIELEEIVPSSRKYPFVNFKAEREMGNDLLTVENLSVTIDGEKI
                     LDNISFILRPGDKTALIGQNDIQTTALIRALMGDIEYEGTIKWGVTTSRSYLPKDNSR
                     DFASGESILEWLRQFASKEEDDNTFLRGFLGRMLFSGDEVNKSVNVLSGGEKVRVMLS
                     KLMLLKSNVLVLDDPTNHLDLESISSLNDGLKDFKESIIFASHDHEFIQTLANHIIVL
                     SKNGVIDRIDETYDEFLENTEVQAKVAQLWK"
     gene            2713..5292
                     /locus_tag="VBC28_001750"
     CDS             2713..5292
                     /locus_tag="VBC28_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000154428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HEO6815713.1"
                     /translation="MTINHYKLRIPYYTISFLLPFIIIVCILFTKNIYWGSPTTILAS
                     DGFHQYVIFNQALRNILHGSNSLFYTFTSGLGLNFYALSSYYLGSFLSPIVYFFNLKN
                     MPDAIYLLTICKIGLIGLSMFVTLCKRHCKVNRVLLLVISTCYSLMSFSISQIEINMW
                     LDVFILIPLVVLGVDQLLWERKPILYFLSLTALFIQNYYFGFMTAIFTSLYFIVQITR
                     NTDSKVAFKQFLHFTFLSLLAGMTSSIMILPTYFDLTTHGEKLTKVSKMFTENSWYMD
                     LFAKNMIGAYDTTKFGSIPMIYVGLLPLLLSLLYFTIKEVPRRTRLAYGFLIIFVIAS
                     FYITPLDLFWQGMHAPNMFLHRYSWVLSVLICLLAAECLEYLDNISWKKILGVNLILV
                     SGFIITFLFKKHYHYLNLELLLLTLTFLSAYIILTISFVSKQIPKLVFYPFLIGFVVL
                     EMTLNTFYQLNSLNDEWIFPSRQGYAKYNHSISKLVRKTERNNSTFFRTERWLGQTGN
                     DSMKYNYNGISQFSSIRNRSSSQVLDRLGFKSDGTNLNLRYQNNTLIADSLFGVKYNL
                     TEYPFDKFGFIKKAQDKQTILYKNQFASQLAILTNQVYQDKPFTVNTLDNQTTLLNQL
                     SGLKETYFEHLIPNSVSGQTTLNKQVFVKKNKQGNTEITYNITIPKNSQLYVSMPFIN
                     FNNEENKIVQISVNNGPFVPNTLDNAYSFFNIGSFAENSRIKVKFQFPHNDQVSFPIP
                     HFYGLKLEAYQKAMTVINKRKVKVRTDHNKVIANYTSPNRSSLFFTIPYDRGWKAYQN
                     NKEIKIFKAQKGFMKINIPKGKGKVTLIFIPYGFKFGVGLSITGIVLFTVYYFKFGKN
                     KIG"
     gene            complement(5360..5433)
                     /locus_tag="VBC28_001751"
     tRNA            complement(5360..5433)
                     /locus_tag="VBC28_001751"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5397..5399),aa:Asn,seq:gtt)
     gene            complement(5463..5534)
                     /locus_tag="VBC28_001752"
     tRNA            complement(5463..5534)
                     /locus_tag="VBC28_001752"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5499..5501),aa:Glu,seq:ttc)
     gene            5726..5799
                     /locus_tag="VBC28_001753"
     tRNA            5726..5799
                     /locus_tag="VBC28_001753"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5760..5762,aa:Arg,seq:tct)
     gene            complement(5871..6350)
                     /gene="rlmH"
                     /locus_tag="VBC28_001754"
     CDS             complement(5871..6350)
                     /gene="rlmH"
                     /locus_tag="VBC28_001754"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEO6815714.1"
                     /translation="MKLKIITVGKLKEKYLKEGVAEYQKRLNRFSKIETIELADEKTP
                     DKASISENQRILDIEGERILSKIGERDYVIGLAIEGKQLPSESFSHLIDQKMISGYST
                     ITFVIGGSLGLSQKVKKRADYLMSFGLLTLPHQLMKLVLMEQIYRAFMIRQGTPYHK"
BASE COUNT         2093 a         1086 c         1048 g         2236 t
ORIGIN      
        1 atgttattat tactaaagtt aaggagctta tcatgtctaa aaaaattaag aattttatac
       61 tattaaatat tggatctcta attgttgcat ttggtttcaa cgctttcttt cttcataatc
      121 atattgtcac gggggggatt agtggtttgg cagttagttt tcatgaagta tttggttggg
      181 attctgcttt ttttattatg attatcaata ttccattgct ccttctttgc tactttggct
      241 taggtaaaca aaccttttta aaaactgtct atggttcttg gatttttcct gtttttatta
      301 agttaacaca aagtgtacca actttgaccc acaacccact cctcgcagca ctttttggag
      361 gtgttattgt aggatgtggt ttggggattg ttttttggag cgactcttca actggtggaa
      421 cggggattat cattcaattc ttaggaaaat atactcctat aagccttgga caaggggtta
      481 tattgattga tggacttgtt acaattgttg gtttcctagc ttttgacagt gatacggtta
      541 tgttttctat tattgggttg ataactatta gttatattat taatgctatc caaactggat
      601 ttacaacctt aagcactgtc ttaatcgttt ctcaagagca ccaaaaaatt aagacatata
      661 tcaatactgt cgcagataga ggagtaacag aaattcccgt taaaggggga tattctggaa
      721 ctaatcaaat catgcttatg acaactattg ctggttatga gtttgctaaa ttacaagagg
      781 caatagcaga aattgacgaa acagccttca taacagtaac tccaacatca caagcttctg
      841 gacgtggatt tagtcttcaa aaaaatcatg gacgtcttga tgaagacatt cttatgccaa
      901 tgtaatcgat tgataattaa agctttttct agtagaatag cagatagaat atacacaaga
      961 ggtaacaaaa ttgcttacag tttcagatgt ttcactacgt tttagtgatc gcaaattatt
     1021 tgatgaagtt aacattaact ttacagcagg aaatacctat ggtcttatcg gagcaaatgg
     1081 cgctggtaaa tcaactttcc ttaaaatttt agctggtgat attgaaccaa caacaggaca
     1141 tattgcactt ggcccagatg aacgcctttc tgtacttcgc caaaatcatt ttgattatga
     1201 agatgagcgc gtcattgatg tcgttatcat gggtaatgaa acactttata gtatcatgaa
     1261 agaaaaggat gctatctaca tgaaagaaga tttttctgat gaagatggtg ttcgcgctgc
     1321 ggaattagaa ggagaatttg ctgaactcgg tggatgggag gctgaaagtg aggcttctca
     1381 attactccaa aatctaaata tttcagaaga gcttcattat caaaatatga gtgaactagc
     1441 taatggagac aaagttaaag ttctccttgc aaaagctctc tttggtaaac cagatgtact
     1501 tctccttgac gagcctacca atggacttga tatccagtct attacttggt tagaagattt
     1561 cttgattgac tttgaaaata cagttattgt agtttcccac gaccgtcatt tcttgaataa
     1621 agtatgtaca catatggctg accttgattt tggaaaaatt aaactgttcg tcggtaacta
     1681 tgatttctgg aaagaatcat cagaattagc agctcgttta caagctgatc gtaatgctaa
     1741 agcagaagaa aaaattaaac aacttcaaga attcgttgca cgattttcag cgaatgcttc
     1801 aaaatcaaaa caagcaactt ctcgtaaaaa aatgcttgat aaaattgaac ttgaagagat
     1861 tgtaccatca agccgtaaat acccattcgt taactttaaa gccgaacgcg aaatgggaaa
     1921 tgatttattg accgttgaaa atctctctgt cactattgat ggggaaaaaa tccttgataa
     1981 tatcagtttc attcttcgtc ctggtgataa aactgcactt attggacaaa atgatatcca
     2041 aactactgca cttattcgag cattgatggg ggatatcgaa tatgaaggaa caatcaaatg
     2101 gggagtaaca actagtcgtt cttaccttcc aaaagacaat tcacgagatt ttgcttctgg
     2161 cgagtcaatc ttagaatggc ttcgtcaatt tgctagtaaa gaagaagacg ataatacctt
     2221 cttacgtggt ttcctaggtc gtatgctttt ctctggagat gaggttaaca aatctgttaa
     2281 tgtcttatct gggggtgaaa aagtacgcgt tatgctttcg aaattaatgc tattaaaatc
     2341 taatgttctt gttcttgatg atccaacaaa ccacttagat ttggaatcta tttcaagtct
     2401 caacgatgga ctaaaagatt tcaaagaatc tattattttt gcaagtcatg accacgagtt
     2461 tattcaaact ttagcgaacc atattattgt tctttctaaa aacggagtta tcgatcgtat
     2521 cgatgaaact tacgatgaat ttcttgaaaa taccgaagta caagctaagg tcgctcaact
     2581 ctggaaataa tcctttggca tatctctaac ctaataaacg tgtcaagatt gaaaatctta
     2641 aaactatatt aatcatttaa ataaaaaggt tggaacaaat agtgtccagc ctttttatct
     2701 taggagatat atatgacaat taaccactat aagcttcgta taccttacta tacaataagc
     2761 tttctacttc ccttcataat catagtctgt atcctcttta ctaagaatat ttattgggga
     2821 agtccaacaa ccatattagc aagcgatggc tttcatcaat atgttatctt taatcaagca
     2881 ttaagaaata ttcttcatgg ttcaaacagc ttattctata cctttacaag cggtttaggt
     2941 ttaaattttt atgctctgag tagttactat ttgggaagtt ttttatcccc aatagtttat
     3001 ttctttaacc taaaaaatat gcctgatgct atctaccttt taacaatatg taaaataggg
     3061 ttgataggtt tgtcaatgtt tgtcactctt tgtaaacgtc attgtaaagt taatcgtgtc
     3121 ttacttttag taatttctac ttgctatagc ttaatgagtt tctcaattag ccaaattgaa
     3181 attaatatgt ggctagatgt atttattcta attccccttg ttgttttagg agttgatcag
     3241 ctactatggg aaagaaaacc tatactttat ttcctctctc tgacagcctt attcatccaa
     3301 aactattatt ttggttttat gactgccatc tttactagcc tttacttcat cgtacaaata
     3361 acccgtaata ctgatagtaa ggttgcattt aaacaatttc ttcattttac ctttttatct
     3421 cttttagctg gtatgactag tagcattatg attttgccaa cttattttga tttaactact
     3481 catggtgaaa agttgacaaa ggtatcgaag atgtttacag aaaattcttg gtacatggat
     3541 ttgtttgcta aaaatatgat tggagcctat gatacaacaa aatttggctc tattcctatg
     3601 atttatgtag gtttacttcc tttactcctt tctttacttt actttacaat aaaggaagtt
     3661 cctcgaagga ctagattagc atatggattt ttaataatat ttgttattgc tagtttttat
     3721 ataacacctc tagatttatt ttggcaaggg atgcatgctc caaatatgtt tctccatcgc
     3781 tactcttggg tactttcagt acttatctgt ctgctagctg ctgaatgcct tgagtattta
     3841 gacaacattt cttggaaaaa gattttaggg gtcaatttaa ttttagtaag tggtttcata
     3901 atcacgtttt tatttaaaaa gcattatcat taccttaatc ttgaattact tcttttaacc
     3961 ttaactttcc tctcagctta tattattcta actattagtt ttgtcagtaa gcaaatccct
     4021 aaattagttt tttatccctt tcttattggt tttgttgtat tagagatgac attaaacacc
     4081 ttttaccaac taaatagtct taacgatgaa tggatatttc catctaggca aggatatgct
     4141 aaatataatc acagcattag caaacttgtc agaaagactg agagaaataa ctctacattc
     4201 ttccgtacag agcgctggtt aggacaaacc ggcaacgact ctatgaaata taattataat
     4261 ggtatttcac aattttcatc tattagaaat cgttcttcaa gtcaagtgct tgatagactt
     4321 ggctttaaat cagatggtac aaatttaaat ttacgttatc aaaataatac tttgatcgca
     4381 gatagtctat ttggtgtgaa gtataacctt acagaatatc cttttgataa atttggtttt
     4441 atcaaaaaag ctcaagataa acaaaccatt ctttacaaaa atcagtttgc cagtcaatta
     4501 gctatactaa cgaatcaagt atatcaagat aaaccattta ctgtgaatac tttggataat
     4561 cagacaactc ttttaaatca attaagtggt ttgaaagaga cgtattttga gcatcttatt
     4621 ccaaatagcg tttctgggca aaccactctt aataaacaag tatttgttaa aaagaataaa
     4681 caagggaata ctgaaattac ctataacata actatcccta aaaacagtca gttatatgtt
     4741 agtatgccgt ttataaattt taataatgag gaaaataaaa ttgttcaaat ctccgttaat
     4801 aatggaccat ttgtacctaa tactcttgat aatgcttatt cattttttaa tattggttcc
     4861 tttgctgaaa acagtcgtat caaagtaaaa tttcaatttc cacacaatga tcaagtttct
     4921 ttcccaattc ctcactttta tgggctcaag ttagaagctt atcaaaaagc tatgactgtt
     4981 attaataaac gaaaagtaaa agtaagaact gatcacaata aagttattgc taattataca
     5041 agtcctaata gaagttctct tttcttcact attccctatg ataggggttg gaaagcttat
     5101 caaaataata aagaaataaa aattttcaaa gctcagaaag gttttatgaa aataaatatt
     5161 cctaaaggaa aaggcaaagt aacattaatt tttatccctt acggctttaa atttggggta
     5221 ggcctatcta ttactggaat agtattattt actgtctatt attttaaatt tggaaaaaat
     5281 aaaataggat gagagtattc tcatcctatt ttattaacaa aaaaacgccg ataaatcaac
     5341 gtttagatgt gtcttatcac tccggcagca ggactcgaac ctgcgacatc atgattaaca
     5401 gtcatgcgct actaccaact gagctatgcc ggataaataa aaaactgcaa agcagttatc
     5461 aatagtccgt acgggattcg aacccgtgtt accgccgtga aaaggcggtg tcttaacccc
     5521 ttgaccaacg gaccataggt taggtgtttc cttagtactc ttaatattat atcagaaaaa
     5581 aaaggtttgt ctatactttt gttcaataat ttttacaaaa aaattgaacc tacttatgag
     5641 caaaaaaata tatttccaac tcataaatag tacatatgaa aacttgacag cctgcctaat
     5701 attttggtag aataacagag ttgatggttc catagctcag ctggatagag cattcgcctt
     5761 ctaagcgaac ggtcgcaggt tcgaatcctg ctggaatcag atattgaaag cctagataga
     5821 ttatctaggc tttttatttg tttttagcag ttgtttcata aatggaacga ttatttgtgg
     5881 taaggagtgc cttgacgaat cataaaagca cgataaattt gttccatcaa gactaatttc
     5941 ataagttgat gaggtaaggt taacagtcca aaactcatta aataatcagc acgttttttc
     6001 actttttgag ataaacccaa actaccacca ataacaaaag taatagtaga gtagccggat
     6061 atcatttttt gatcgattag atggctaaat gattcagatg gcaattgttt cccttctata
     6121 gctaaaccaa tgacataatc cctctcacct attttactca aaatacgttc cccttctata
     6181 tctaaaattc tttgattttc agatatacta gctttatcag gtgtcttttc atcagctagc
     6241 tcaattgtct caattttaga aaagcgattc agacgtttct gatactctgc aacaccttct
     6301 ttaaggtatt tctctttcaa ttttccaact gtaattattt ttaatttcat agctacatta
     6361 taacataatc cacaatattt tcacaattta tccacatgtt tatccttaca tatcccctta
     6421 ttttataggt attctataac tataatcaag gttatccaca agt
//
LOCUS       DATRMA010000087         6322 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.087, whole genome shotgun sequence.
ACCESSION   DATRMA010000087 DATRMA010000000
VERSION     DATRMA010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6322
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.087"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            <1..1047
                     /locus_tag="VBC28_001755"
     CDS             <1..1047
                     /locus_tag="VBC28_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEO6815715.1"
                     /translation="LTQRQFDDLTDIQNKLFQPITDGDNILGISPTGTGKTLAYLFPT
                     LLKLQPKKSQQLLILAPNSELAGQIFDVTKEWAEPLGLTAQLFLSGSSQKRQIERLKK
                     GPEILIGTAGRVFELVKLKKIKMMNINTIVLDEFDELLGDSQYHFVDNIINRVPRDQQ
                     MIYISATNKLDNSKLADNTITIDLSNQKLDTIKHYYITVDKRERTDLLRKFSNIPDFR
                     GLVFFNSLSDLGACEERLQFNRASAVSLASDINIKFRKVILEKFKNHDISLLLGTDLV
                     ARGIDIDNLEYVINFDIARDKETYTHRSGRTGRMGKEGCVITFVTHKEELKQLKKYAT
                     VTELVLHNQKLHLK"
     gene            complement(1080..2333)
                     /locus_tag="VBC28_001756"
     CDS             complement(1080..2333)
                     /locus_tag="VBC28_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEO6815716.1"
                     /translation="MKKLIIGIFSLIIIAILAWQGFSFLKHKEIKLQQAVVEKEIRIA
                     EKTVEVVKRQKTERVLFLEPKGYDKSLSADILKWNQKSFEHKKFYDNQYIILRPQLAD
                     SNFANVKKLSIYQILYQKEKGSMFQKSSRLLRTYLLDQNKKPFELDELLAHNISGFKA
                     ILENIAPGTQLKEHDSNKEFLKTGRVTDGLDVKDGKLIINDLKLPLDKLYNVIDESYL
                     KSSDLDLVSNLKAKAPRVALTFDDGPNEKTTPKALEILKRYNAKATFFVMGQSAVGHT
                     DILQRMHAEGHEIGNHTWDHPNLTKLPAEKIKEEIHKTNDLIMKATGQKPVYLRPPYG
                     ATNATVKTVTGLKEMLWSVDTEDWKNHNTQAMMTNIKKQLRPGGVILMHDIHQTTIDA
                     LPTIMDYLTTQGYYFVTVGELYSTP"
     gene            complement(2473..3900)
                     /locus_tag="VBC28_001757"
     CDS             complement(2473..3900)
                     /locus_tag="VBC28_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HEO6815717.1"
                     /translation="MTKEYQNYVNGEWKSSVNQIEILSPIDDSSLGFVPAMTREEVDH
                     AMKAGREALPAWAALTVYERAQYLHKAADIIERDKEEIATVLAKEISKAYNASVTEVV
                     RTADLIRYAAEEGIRLSTSADEGGKMDASTGHKLAVIRRQPVGIVLAIAPYNYPVNLS
                     GSKIAPALIGGNVVMFKPPTQGSVSGLVLAKAFAEAGLPAGVFNTITGRGSEIGDYIV
                     EHEEVNFINFTGSTPVGKRIGKLAGMRPIMLELGGKDAGVVLADADLDNAAKQIVAGA
                     YDYSGQRCTAIKRVLVVEEVADELAEKISENVAKLSVGDPFDNATVTPVIDDNSADFI
                     ESLVVDARQKGAKELNEFKRDGRLLTPGLFDHVTLDMKLAWEEPFGPILPIIRVKDAE
                     EAVAIANKSDFGLQSSVFTRDFQKAFDIANKLEVGTVHINNKTGRGPDNFPFLGLKGS
                     GAGVQGIRYSIEAMTNVKSIVFDMK"
     gene            complement(4050..5783)
                     /gene="ptsP"
                     /locus_tag="VBC28_001758"
     CDS             complement(4050..5783)
                     /gene="ptsP"
                     /locus_tag="VBC28_001758"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEO6815718.1"
                     /translation="MTEMLKGIAASDGVAVAKAYLLVQPDLSFETVTVEDTNAEEARL
                     DVALQASQDELSVIREKAVESLGEEAAAVFDAHLMVLSDPEMINQIKETIRAKQVNAE
                     TGLKEVTDMFITIFEGMEDNPYMQERAADIRDVAKRVLAHLLGVKLPNPATINEESIV
                     IAHDLTPSDTAQLNKQFVKAFVTNIGGRTSHSAIMARTLEIAAVLGTNDITERVQDGQ
                     LIAVNGITGEVIIEPTEAQISAFKAAGEAYAKQKAEWALLKDAQTVTADGKHFELAAN
                     IGTPKDVEGVNENGAEAVGLYRTEFLYMDSQDFPTEDEQYEAYKAVLEGMNGKPVVVR
                     TMDIGGDKELPYFDLPKEMNPFLGFRALRISISETGDAMFRTQIRALLRASVHGQLRI
                     MFPMVALLKEFRAAKAIFEEEKANLLADGVAVAEGIEVGIMIEIPAAAMLADQFAKEV
                     DFFSIGTNDLIQYTMAADRMNEQVSYLYQPYNPSILRLINNVIKAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSVLRTRSLMKKLDTAKMEEYANRALSECSTMEEV
                     IELQKEYVDFD"
     gene            complement(5788..6051)
                     /locus_tag="VBC28_001759"
     CDS             complement(5788..6051)
                     /locus_tag="VBC28_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019775469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HEO6815719.1"
                     /translation="MASKDFHIVAETGIHARPATLLVQTASKFASDITLDYKGKAVNL
                     KSIMGVMSLGVGQGADVTISAEGADADDAIAAIEETMTKEGLA"
BASE COUNT         1947 a         1245 c         1023 g         2107 t
ORIGIN      
        1 ttaacccagc gtcaatttga tgatctaaca gatattcaaa ataaactttt tcaacctatt
       61 actgatggtg ataatatcct tggaattagc cctacaggta ctggtaaaac tctagcatac
      121 cttttcccaa cgcttcttaa gttgcaacct aaaaagagcc aacaactctt aattttagct
      181 ccaaattcag aattagctgg acaaatcttc gacgttacaa aagaatgggc tgaaccatta
      241 ggactgaccg cacaactatt cctttcaggt tctagtcaaa aacgccaaat tgagcgacta
      301 aaaaaaggac cagaaattct tataggaact gctggacgcg tatttgaact tgtgaaacta
      361 aaaaaaataa aaatgatgaa cattaatact atcgtccttg atgaattcga tgaattactc
      421 ggcgactcac aatatcattt tgtggacaat attattaatc gcgttcctcg tgatcagcaa
      481 atgatttaca ttagtgccac caacaaactg gacaatagta aacttgccga taacactata
      541 acaatcgatc tatctaatca aaaacttgat actataaaac attattatat tacagttgat
      601 aaaagagagc gtactgacct acttcgtaaa ttttcaaata ttcctgattt tagaggtctt
      661 gtctttttta acagcttatc agacttaggt gcatgtgaag aacgtttaca atttaatagg
      721 gcctcggcag tttctttagc aagcgatatt aatattaaat tccgtaaagt tatcttagag
      781 aaatttaaaa accatgatat ttcattactc ctaggaactg atttggtagc tcgtggcatt
      841 gatatcgaca acctagaata tgtgattaat tttgatatcg cacgcgacaa agaaacatat
      901 acacatcgtt ctggacgaac tggtcgtatg gggaaagagg gatgtgttat tacatttgta
      961 acacataaag aagagctcaa acaacttaaa aaatacgcca ccgttacaga acttgtactt
     1021 cacaatcaaa aattacattt aaaataaaaa aagaaacaag taacttgttt ctttttttat
     1081 taaggcgttg aatataattc acctacagta acaaaataat agccctgggt tgtcagataa
     1141 tccataattg taggcagagc atctatggtt gtctggtgaa tatcatgcat caaaatcact
     1201 cctccaggac gtaattgttt tttgatattg gtcatcattg cttgtgtatt atgatttttc
     1261 cagtcttctg tatcaactga ccacaacatt tctttcaatc ctgtcactgt ttttacagta
     1321 gcatttgttg ctccgtaagg gggacgtaag tacaccggtt tttgtcctgt tgctttcata
     1381 atcagatcat tagtcttatg aatctcttcc ttgattttct ccgctggtag tttggttaaa
     1441 ttaggatgat cccatgtatg attaccaatc tcatgacctt cagcatgcat cctttgtaaa
     1501 atatctgtgt gccctacagc actttgtccc attacaaaga aagtcgcttt agcattatat
     1561 cttttaagga tttctaaggc tttaggagtc gttttttcat ttggtccatc atcaaaggta
     1621 agtgcaacac gtggtgcttt agcttttaaa ttagaaacta aatctagatc acttgatttt
     1681 aaatatgact catcgatcac attatataat ttatcaagtg gtaattttaa atcattaata
     1741 attaacttac catctttaac atctagccca tctgttactc ttccagtttt taaaaattct
     1801 ttattgctat catgttcctt tagttgagtt cctggagcta tattttctaa aatagcctta
     1861 aatccagata tattatgagc taaaagctca tctaattcaa aaggtttctt attttgatca
     1921 agtaagtagg ttctcaaaag gcgacttgat ttttgaaaca tacttccttt ttctttttga
     1981 tataagattt gataaatgga taatttttta acatttgcaa agttagaatc tgctaattga
     2041 ggacgtagaa taatgtattg attgtcataa aattttttat gctcaaagct tttttgattc
     2101 cactttaaaa tatcagcgct tagacttttg tcatatcctt taggttcaag aaaaagaaca
     2161 cgttcagttt tctgtctttt caccacttct accgttttct cggctatgcg aatttctttt
     2221 tcaacaacag cttgttgaag ttttatttct ttatgtttca aaaacgaaaa accttgccaa
     2281 gcaagaatcg ctataataat tagactaaaa atacctataa ttaatttctt catataatga
     2341 ctcttttgtt attttttatg acaattatat cacaaaaata aaaccacttt agaagattat
     2401 ataattcttt atttttaatg taaaagaggt ttataaaacg actcaactga caatagttga
     2461 gtcgttttac gtctatttca tatcaaaaac tatcgatttt acatttgtca ttgcttcaat
     2521 tgaatatctg ataccttgaa cacctgcacc agatcctttg agtcctaaga atgggaaatt
     2581 atcaggacca cgtccagtct tattgttaat gtgaactgta ccaacttcaa gtttatttgc
     2641 tatatcaaat gctttttgga aatcacgtgt aaagactgat gattgtaatc caaaatcaga
     2701 tttgttggca atagcaacag cttcttctgc atccttgaca cgaataattg ggagaattgg
     2761 tccaaaaggc tcttcccaag ctagtttcat atctaaagta acatgatcaa acaatcctgg
     2821 agttaataga cgaccatcac gtttaaattc attcaattct ttcgcacctt tttgacgtgc
     2881 atctactact aagctttcaa taaagtcggc tgaattatca tcaataaccg gtgtcaccgt
     2941 tgcattatca aatggatcac ctactgataa ttttgctaca ttttcagata ttttttctgc
     3001 caattcatct gcaacttctt caacgacaag tacacgctta attgccgtac agcgttgtcc
     3061 ggagtaatca taagcacctg caacgatttg tttagcagcg ttgtcaaggt cagcatcagc
     3121 taagacgaca cctgcatcct taccacccaa ctcaagcata attggacgca ttcctgctaa
     3181 cttaccaata cgttttccaa ctggcgttga tcctgtaaag ttaataaaat taacttcttc
     3241 atgctcaaca atgtaatctc caatctcaga accgcgtcct gtaatagtat taaagacacc
     3301 tgctggaaga cctgcttctg caaaagcttt tgctaaaaca agtcctgaga ctgaaccttg
     3361 tgttggtggt ttaaacatca caacgtttcc accaattaga gctggcgcaa tttttgatcc
     3421 tgagaggtta acagggtaat tataaggtgc gattgctaaa acgataccta ctggttgacg
     3481 acgaataaca gccaacttat gacctgttga agcatccatt tttccacctt cgtcagctga
     3541 agttgataaa cgaattcctt cttctgctgc ataacgaata agatcagctg tccttacaac
     3601 ctcagttact gaagcattgt aagctttaga aatttctttt gctaaaacag tagcaatttc
     3661 ttctttatca cgttcaataa tgtctgcggc tttatgaagg tattgtgcac gttcatatac
     3721 tgtcaaagca gcccaggctg gtaaagcctc acgacccgct ttcatagcat gatcaacttc
     3781 ttctcgagtc atcgctggca cgaatcccaa tgaagaatca tcaattggtg acaaaatctc
     3841 aatctgatta acagatgatt tccattcgcc attgacataa ttttgatatt cttttgtcaa
     3901 agtaaatctc cttttacatt gtaataggtt tattctatca ctttatctat ttatttcaat
     3961 tatctgacat atttttctta gaaacttaac aatatccaaa ctttcaataa aaaagtagag
     4021 attttttaat ccctactttt ttattattct cagtcaaagt caacgtattc tttttgaagt
     4081 tcgataactt cttccatagt tgaacactct gaaagagcac gattagcgta ttcttccatt
     4141 ttagctgtgt ccaatttttt cattaagcta cgtgtacgaa gtactgaggt tgctgacatt
     4201 gagaattcat ccaatcccat accaacaaga agtggaaccg cagtttgatc accggccatc
     4261 tcaccacaca tacctgccca tttaccttca gcatgagcag ctttgataac gttgttaatc
     4321 aaacgaagga ttgatggatt gtatggttga tagaggtatg aaacttgctc attcatacgg
     4381 tccgcagcca ttgtgtattg gataagatca ttagtaccaa ttgagaagaa atcaacttct
     4441 tttgcaaatt ggtctgcaag catagctgcc gctgggattt caatcataat accaacttca
     4501 atgccttcag ctactgctac accgtcagct aacaagttcg ctttctcttc ttcaaatata
     4561 gctttagcag cacggaattc tttaagcaaa gcaaccattg ggaacatgat gcgtagctgt
     4621 ccatgaactg aagcacgaag aagtgctcgg atttgtgtgc ggaacattgc atcaccagtt
     4681 tcagaaattg aaatacgcaa tgcacggaat cctaagaatg ggttcatctc ttttggaaga
     4741 tcaaagtaag gaagctcttt atctccaccg atatccattg tacgaacaac aactggttta
     4801 ccattcatac cctcaagaac tgctttatat gcttcatatt gctcatcttc tgttgggaaa
     4861 tcttgtgaat ccatatataa gaactctgta cggtaaagac caactgcttc tgctccattt
     4921 tcgttaacac cctcaacatc tttaggtgta ccgatattag cagctaattc aaaatgttta
     4981 ccatcagcag taactgtttg tgcatcctta agaagtgccc attcagcttt ttgtttggca
     5041 tatgcttcac ctgctgcctt gaaggctgaa atttgagctt cagttggttc aatgataact
     5101 tcaccagtaa taccattaac agcaatcaac tgaccatctt gaacacgttc agtaatatca
     5161 tttgtaccaa gaacagctgc gatttcgagt gtacgtgcca taatagctga gtgacttgta
     5221 cgccctccaa tatttgttac aaaagcttta acaaattgtt tatttaattg agctgtatca
     5281 gaaggtgtca aatcgtgtgc aataacgatt gattcttcat tgatagttgc tgggtttggt
     5341 aatttaacac ccaaaagatg tgctaagaca cgtttggcaa catcacggat atctgctgca
     5401 cgttcttgca tgtatgggtt atcttccata ccttcaaaga tagtgatgaa catatcagtc
     5461 acttctttaa gacctgtttc tgcatttact tgttttgcac gaatagtctc tttaatttga
     5521 ttaatcattt ctgggtcaga aagaaccatt aaatgagcat caaatacagc tgcggcttct
     5581 tcgcctaagc tttctactgc cttctcacgg ataacagaaa gctcgtcttg tgaagcttgg
     5641 agtgcaacat caaggcgagc ttcttctgcg tttgtatctt cgactgttac agtctcaaat
     5701 gacaaatccg gttggacaag tagatatgct ttagcaactg caacaccatc agaggctgcg
     5761 attcctttaa gcatttctgt cattttctta tgccaatcct tcttttgtca ttgtttcttc
     5821 gatagctgca attgcatcat ctgcatctgc accttctgct gaaatagtaa catcagcacc
     5881 ttgaccaaca ccaagactca taacacccat gattgattta aggtttactg ctttaccttt
     5941 gtaatcaagc gtgatgtctg aagcaaattt gctagctgtt tggacaagca aagtagctgg
     6001 acgtgcgtga atacctgttt ctgcaacaat gtggaaatct tttgaagcca tattatggtt
     6061 ctccttttgt catttgtaaa tatttgagtc acctattggt aacccttaca agttaaaagt
     6121 ataacatatt gctttttatt tttcaagatt aaatgtctca aacagaaaaa aacttgaatg
     6181 ttttccacag cttttcatat taaaaatgag agtcttagaa tttaaaaata gaattctaaa
     6241 aatgattttt ttatgttctt ctgaaaggaa ttggaaaaca ggctgaaagc ttttaaacta
     6301 accctaaaaa acgaaatata gt
//
LOCUS       DATRMA010000088         6164 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.089, whole genome shotgun sequence.
ACCESSION   DATRMA010000088 DATRMA010000000
VERSION     DATRMA010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6164
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.089"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            266..1087
                     /locus_tag="VBC28_001760"
     CDS             266..1087
                     /locus_tag="VBC28_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEO6815720.1"
                     /translation="MIKVIATDMDGTFLRSDKTYDKARFSSLLTLMEKYDIKFVAASG
                     NLYDQLLLNFLEYPNRIAYVAENGGRVIDQDGTLLKETYLSNDTVAAVLSYLYQNYPE
                     TLISLSGEKRSYLERRTPINRRTELEYYMPNFIYKDHLLPLDDDRYFQMTLWVNENLV
                     SEMLLDISEHFKNHHIRLTSSGFGCIDVLPADVNKADGIAILLEKWGLKQDQVMVFGD
                     GGNDVEMLRAANISYAMSNAPEEIKAIAKYQTVSNDQDGVLETIENFLHKMSDNE"
     gene            1188..1802
                     /locus_tag="VBC28_001761"
     CDS             1188..1802
                     /locus_tag="VBC28_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HEO6815721.1"
                     /translation="MLEVIDKALRDYQMKREQFFEINNQTVQEGAIVFTGDSIVEFFP
                     LKKHLGRDYPLVNRGVAGSDTYWLLENLRTQVWELLPSKVFILIGTNDIGLGHSQSEI
                     IANITDIIAEIRAESYMTEINILSVLPVSEEDDYIERVKVRNNQAIKALNKTLSVISG
                     INYIELYDLLVDEKGQLASSFTKDGLHLTDQAYAKISETIKPHL"
     gene            1965..3401
                     /locus_tag="VBC28_001762"
     CDS             1965..3401
                     /locus_tag="VBC28_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEO6815722.1"
                     /translation="MVKQVFPKGFLWGGATAANQCEGAYNVDGRGLANVDVVPTGEDR
                     FAIISGQKKMFDFEEGYFYPAKESIDFYHHYKEDLALLAEMGFKTYRMSIAWTRIFPK
                     GDELYPNEAGLRFYENIFKECRKYGIEPLVTITHFDCPIYLIKHYGGWRSRKMIGFYE
                     RLVRALFTRFKGLVKYWLTFNEINMILHAPFMGAGLYFEDGENQEQIKYQAAHHELVA
                     SAIAVKIAHEVDPNNQIGCMLAAGQYYPNTCHPQDYWASMQKNRENYFFIDVQTRGKY
                     PNYAKKYFEHLGISIQMTAEDLALLRDYTVDFISFSYYSSRVASGNPTVSEQVQENIF
                     ASLKNPYLKSSEWGWQIDPLGLRITLNAIWDRYQKPMFIVENGLGAVDIPDENGYVED
                     DYRIDYLRQHIAAMRDAIYVDGVNLIGYTTWGCIDLVSAGTGEMEKRYGFIYVDRNNK
                     GEGTLKRYKKKSFYWYKKVIASNGSQIE"
     gene            3492..4625
                     /locus_tag="VBC28_001763"
     CDS             3492..4625
                     /locus_tag="VBC28_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000667240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEO6815723.1"
                     /translation="MKFEKRQVYYVVITFAICYAIQAYWGAVSNILTTLHKAIFPFLM
                     GAGIAYIINIVMSVYERLYIKLFKGSRLLMAIKRSVSMILSYATFIGLIVWLFSIVIP
                     DLISSLSSLLVIDTGALAKLVNNLNENKQISEALNYMGTDKDLVSTLSGYSQQILKQV
                     LSVLTNLLTSVSSIAATLLNVFVSFIFSIYVLANKEQLGRQFNLLIDTYLGSTGKTFH
                     YVRHILHQRFHGFFVSQTLEAMILGSLTVIGMLIFQFPYALTVGVLVAFTALIPVVGA
                     YIGVTIGFILIATESLTEAFLFVLFLILLQQFEGNVIYPKVVGGSIGLPSMWVLTAIT
                     IGGALWGILGMLLAVPVAATIYQIVKDHIIKRQTLRNRARTYR"
     gene            4721..5401
                     /gene="radC"
                     /locus_tag="VBC28_001764"
     CDS             4721..5401
                     /gene="radC"
                     /locus_tag="VBC28_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEO6815724.1"
                     /translation="MYHIELKKEALLPRERLVDLGADRLSNQELLAILLRTGIKEKPV
                     LEISTQILENISSLADFGQLSLQELQSIKGIGQVKSVEIKAMLELAKRIHKAEYDRKE
                     QILSSEQLARKMMLELGDKKQEHLVAIYMDTQNRIIEQRTIFIGTVRRSVAEPREILH
                     YACKNMATSLIIIHNHPSGSPNPSESDLSFTKKIKRSCDHLGIVCLDHIIVGKNKYYS
                     FREEADIL"
     gene            complement(5414..6052)
                     /locus_tag="VBC28_001765"
     CDS             complement(5414..6052)
                     /locus_tag="VBC28_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEO6815725.1"
                     /translation="MIMDKSIPKATAKRLSLYYRIFKRFNTDGIEKASSKQIADALGI
                     DSATVRRDFSYFGELGRRGFGYDVKKLMNFFAEILNDHSTTNVMLVGCGNIGRALLHY
                     RFHDRNKMQISMAFDLDSNDLVGKTTEDGIPVYGISTINDHLIDSDIETAILTVPSTE
                     AQEVADILVKAGIKGILSFSPVHLTLPKDIIVQYVDLTSELQTLLYFMNQQR"
BASE COUNT         2031 a          913 c         1160 g         2060 t
ORIGIN      
        1 attttattat gactattgta atagttacga atctattatt caactcaaca ataagaaaac
       61 tcttcagtaa atcattatgt taaacaagct ctgatagtat ggagataagt ataggtaaaa
      121 gtcgatttaa tctctgaaac aaaatttaac ttaattaata gaaccgttga cgtatcaggt
      181 atcaaatctt tttggtttat atgtgatata atgaaagaac atattgagat atttggcacc
      241 ctcttgaaag gcaaaggtta cttgtatgat taaagtaata gcaacagata tggatggaac
      301 ttttctacgc tcagataaaa cttatgataa agcacggttt tcttctttat taacgttgat
      361 ggagaagtat gatattaaat ttgtagctgc tagtggtaat ttatacgatc aattgttgtt
      421 aaactttttg gaatacccta atcgtatcgc ctacgtcgct gaaaatggtg gtcgtgtcat
      481 tgaccaagat ggcacattac taaaagaaac ttatttatct aatgatacag ttgcagctgt
      541 tttatcctat ttatatcaga actatcctga gacactcatc agcttatctg gtgaaaaacg
      601 ttcatacctt gagcgaagaa ctcctattaa taggcggact gaattagaat actatatgcc
      661 taattttatc tataaagacc atttgctgcc cttagatgat gaccgatatt ttcaaatgac
      721 actttgggta aatgaaaatc ttgtttctga gatgttgtta gatattagtg aacactttaa
      781 aaatcatcat atcagattga cctctagtgg ttttggttgt atcgatgtct taccagctga
      841 tgtcaataaa gcggatggta tcgctattct ccttgaaaag tggggattga aacaggatca
      901 agtgatggtt tttggtgatg ggggcaatga tgtggaaatg ttgagagcag caaatatttc
      961 ttacgctatg tcaaatgcgc cagaagaaat taaagctatt gctaaatatc aaacagttag
     1021 taacgatcaa gatggtgtgt tagaaacgat agagaacttt cttcacaaga tgagtgataa
     1081 tgaatagaac cacgtagctt ttcacgaagc atactattca attctcaggt gaaattttgt
     1141 tataatacaa gaaagcctag agaaatattt aagaatgaag gagaacgatg ttagaagtta
     1201 ttgacaaagc tttaagagat tatcaaatga aacgagaaca gttttttgag attaacaatc
     1261 agacagtaca agaaggtgcc attgttttca caggagattc tatcgttgag ttttttcctt
     1321 taaaaaaaca tttaggacgt gactatcctt tagttaatag aggagtagca ggatcagata
     1381 cttattggct tttagaaaac ttgagaaccc aagtatggga actattacca tccaaagtat
     1441 ttattttaat tggaacaaat gatattggtt tagggcattc acagtcagaa atcatagcta
     1501 atatcacgga tataattgca gaaattcgtg cggaatctta tatgacagag attaatatac
     1561 tgagtgtttt acctgttagt gaagaagatg actatataga gcgtgtcaag gtaagaaata
     1621 atcaagcaat aaaagcttta aacaaaacct tatcagtaat ttctggcatt aattatattg
     1681 aattgtatga tttattagtt gacgaaaaag gtcagcttgc tagcagtttt acaaaagatg
     1741 gtttgcacct aacagatcaa gcctatgcta aaatttctga aacgataaaa ccccaccttt
     1801 aaaagtgttt agatactatt tttaagtatt taaatacaca gaattagtga aaagaccttc
     1861 taagtagaaa gttcatgtca cggatagtac cagtatttgg cgaatcaatg tcagtaactg
     1921 gtctttttta atattatgat aagaaaaaac aagaggggta aatgatggta aaacaagtat
     1981 ttccaaaagg ttttttatgg ggcggggcaa ctgctgccaa tcaatgtgaa ggagcttaca
     2041 atgttgatgg acgtggccta gcaaatgtag atgttgttcc tactggagaa gatcgatttg
     2101 caattatttc aggacaaaag aaaatgtttg attttgagga aggatacttt tatccagcaa
     2161 aagaatcaat agatttttat catcattata aagaagacct tgcattactt gcagaaatgg
     2221 gtttcaaaac ctatcgtatg tcaatagcat ggacgcgtat ttttccaaag ggtgatgagc
     2281 tatatccaaa tgaagctggt cttcggtttt atgaaaatat ttttaaagag tgtcgtaagt
     2341 atggtattga acctttggta accatcacac attttgactg tcctatctac cttattaaac
     2401 attacggtgg gtggcggagc cgtaaaatga ttggttttta tgagcgcctt gtacgagctt
     2461 tatttactcg ttttaagggg ttagttaaat attggttgac ctttaacgaa atcaatatga
     2521 ttttacacgc tccttttatg ggcgctggct tatattttga agatggtgaa aatcaagagc
     2581 aaattaaata tcaagcagct caccatgagt tagtggcttc ggctattgca gtaaaaattg
     2641 ctcatgaagt tgatccaaat aatcaaatcg gatgtatgtt agctgcaggt caatactatc
     2701 caaatacgtg tcatccacaa gattattggg cttcgatgca aaaaaataga gaaaattatt
     2761 tttttattga tgtgcaaacg cgtggcaaat accctaatta tgctaaaaaa tattttgagc
     2821 atttaggtat ttcaattcaa atgacagcag aagatcttgc tttgttaaga gactatacgg
     2881 ttgattttat ttcattttct tactattcaa gccgagtagc ttcaggtaat ccaactgtca
     2941 gtgaacaggt tcaagaaaat attttcgcat ctcttaagaa tccttacttg aaatcttctg
     3001 aatggggatg gcaaattgat ccgcttggat tacgcattac cttaaatgct atctgggatc
     3061 gttatcaaaa accgatgttt attgttgaaa atggacttgg agcagtagat attcctgatg
     3121 agaatggtta cgtagaagat gattatcgta ttgattacct ccgtcagcac attgctgcaa
     3181 tgagagatgc tatatatgta gatggtgtta atctaattgg ctatactaca tggggatgta
     3241 ttgatttagt atctgcagga actggtgaaa tggaaaaacg ctatggtttc atttatgttg
     3301 atcgcaataa taaaggagag ggaacactca agcgttacaa gaaaaaatcg ttttattggt
     3361 acaagaaggt tattgcaagt aatggcagtc aaatagaata aagagcagac taataactgt
     3421 taactctctt tttctcattc ataaaatagt gtataataat tgaaagaaat taagattaat
     3481 aaggagattt catgaaattt gaaaaacggc aggtctatta tgttgtcata acatttgcta
     3541 tttgctatgc tatacaggct tattggggag ctgtttctaa tattttaact acgcttcata
     3601 aggcaatatt tccttttttg atgggagctg gaattgccta tattattaat attgtaatgt
     3661 cagtctatga gcgattatat ataaagcttt ttaaaggatc tagactatta atggcaatca
     3721 agcgtagtgt ttctatgatt ttatcctatg caacttttat tggtttaatt gtctggctat
     3781 tttcaattgt cattccagat ttgatttcta gtttgagttc tttattggtt attgataccg
     3841 gagcacttgc taaattggtt aataatctca atgaaaataa acaaatttct gaggctttaa
     3901 attatatggg aacagataaa gacttagttt ctactttaag tggttatagc cagcagattt
     3961 tgaagcaagt tttatctgtt ttaacaaatt tactaacctc agtttcctct attgcggcaa
     4021 cacttctgaa tgtttttgtt agttttattt tttcaattta cgttttggca aacaaggagc
     4081 agttgggacg tcaatttaat ttgttaattg atacctattt aggttcaaca ggcaaaacat
     4141 tccattacgt tcgtcatatc cttcatcaac gtttccatgg tttttttgta agccaaactt
     4201 tagaagctat gattttagga agtttgacgg ttattggtat gttgattttc caatttcctt
     4261 atgctttaac agttggggtt ttagttgctt ttacagctct aataccggtt gtgggagcct
     4321 acattggtgt tacaatcggt ttcatcttaa ttgctactga atcgcttact gaagcattct
     4381 tgtttgttct tttcttgatc cttttacaac aatttgaggg aaatgtcatt tatccgaaag
     4441 ttgtcggtgg atcgattgga ctgccttcta tgtgggtttt aacggctatt actatcggag
     4501 gtgctttatg ggggatctta ggcatgttac ttgctgttcc tgttgcagct actatctatc
     4561 agattgtaaa agatcatatt atcaagcgac aaacgcttag aaatcgtgca cgaacctatc
     4621 gttaaataaa aaatacttag atttttcatc taagtatttt ttattgattt aaaacatgag
     4681 tttttgattg gtttacgaat attatgactg gaggtgtttt atgtaccata ttgaattaaa
     4741 aaaggaagct ttactaccaa gagaacgcct agttgattta ggcgcagata gattgagtaa
     4801 tcaggagtta ttagccattc tcttacgtac aggtattaaa gaaaaacctg ttcttgaaat
     4861 ttcaacgcaa attttagaaa acataagcag tttagcagat tttggtcaat tatccttaca
     4921 ggagttgcaa tccattaaag gaatcggtca ggttaaatcc gtcgaaataa aagctatgct
     4981 agaactagca aaacggattc acaaagctga atatgatcgt aaagagcaaa ttttaagtag
     5041 tgaacaatta gcgaggaaaa tgatgctcga attaggggat aaaaaacaag aacatttagt
     5101 agctatttat atggatacac aaaatcgtat tatcgaacag agaactattt ttattggtac
     5161 tgtacgtcgt tcagtagcag agccaagaga aattctacat tatgcttgta aaaacatggc
     5221 aacttctttg attattatac ataatcatcc ctcaggttct ccaaatccca gtgaaagtga
     5281 tttaagtttc actaaaaaaa taaaacgatc atgtgatcat ctgggaattg tctgcctaga
     5341 tcacatcatc gttggaaaaa ataaatatta tagttttcga gaagaagcag atattttata
     5401 aataatgaac tatttatcgc tgctggttca tgaaataaag taaagtttgt aattcgcttg
     5461 ttaaatctac atactgaaca atgatatctt ttggtaatgt taaatgaact ggagaaaaac
     5521 tcaagatgcc ttttatacct gctttgacta agatgtcagc aacttcttgg gcttctgtac
     5581 taggtactgt taggatagca gtttcaatat cactatctat taaatggtca ttgatagtcg
     5641 aaataccgta gacaggaatg ccatcctcgg ttgttttacc aactaaatca ttgctatcta
     5701 aatcaaaagc cattgaaatt tgcattttat tgcgatcgtg gaaacgataa tgcaagagag
     5761 ctctaccgat atttccacac cccaccagca taacatttgt tgtagaatga tcgttcaata
     5821 tttctgcaaa gaagttcata agttttttga catcataacc aaaaccacgg cgtcctagtt
     5881 caccaaaata agaaaaatca cgtcgaacag tagcagaatc gatacctagg gcatctgcaa
     5941 tttgtttgga actagctttt tcgatgccat cagtattaaa acgtttaaaa atacggtagt
     6001 acagtgataa acgtttagca gttgctttag gaatagactt atccataatc acaaaattat
     6061 ctccttgtta aaaattttat tattcgattt agaatcagtt atataactta attcttcaca
     6121 tctctattgt acaaaaagat tgtgaaagtg acaacaaaat ggtt
//
LOCUS       DATRMA010000089         6096 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.090, whole genome shotgun sequence.
ACCESSION   DATRMA010000089 DATRMA010000000
VERSION     DATRMA010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6096
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.090"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            <1..999
                     /locus_tag="VBC28_001766"
     CDS             <1..999
                     /locus_tag="VBC28_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEO6815726.1"
                     /translation="ADTSNNEILSKIQQQFETILTKENSIVNGTIEDPMGDKINLQLG
                     NGQTLQPSDYTLQGNDGSVMKDGIATGGPNNDGGILKGVKLEYIGNKLYVRGLNLGEG
                     QKVTLTYDVKLDDSFISNKFYDTNGRTTLNPKSEDPNTLRDFPIPKIRDVREYPTITI
                     KNEKKLGEIEFIKVDKDNNKLLLKGATFELQEFNEDYKLYLPIKNNNSKVVTGENGKI
                     SYKDLKDGKYQLIEAVSPEDYQKITNKPILTFEVVKGSIKNIIAVNKQISEYHEEGDK
                     HLITNTHIPPKGIIPMTGGKGILSFILIGGAMMSIAGGIYIWKRYKKSSDMSIKKD"
     gene            1129..3246
                     /locus_tag="VBC28_001767"
     CDS             1129..3246
                     /locus_tag="VBC28_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HEO6815727.1"
                     /translation="MKRINKYFAMFSALLLTLTSLLSVAPAFADEATTNTVTLHKILQ
                     TESNLNKSNFPGTTGLNGKDYKGGAISDLAGYFGEGSKEIEGAFFALALKEDKSGKVQ
                     YVKAKEGNKLTPALINKDGTPEITVNIDEAVSGLTPEGDTGLVFNTKGLKGEFKIVEV
                     KSKSTYNNNGSLLAASKAVPVNITLPLVNEDGVVADAHVYPKNTEEKPEIDKNFAKTN
                     DLTALTDVNRLLTAGANYGNYARDKATATAEIGKVVPYEVKTKIHKGSKYENLVWTDI
                     MSNGLTMGSTVSLKASGTTETFAKDTDYELSIDARGFTLKFTADGLGKLEKAAKTADI
                     EFTLTYSATVNGQAIIDNPESNDIKLSYGNKPGKDLTELPVTPSKGEVTVAKTWSDGI
                     APDGVNVVYTLKDKDKTVASVSLTKTSKGTIDLGNGIKFEVSGNFSGKFTGLENKSYM
                     ISERVSGYGSAINLENGKVTITNTKDSDNPTPLNPTEPKVETHGKKFVKTNEQGDRLA
                     GAQFVVKNSAGKYLALKADQSEGQKTLAAKKIALDEAIAAYNKLSATDQKGEKGITAK
                     ELIKTKQADYDAAFIEARTAYEWITDKARAITYTSNDQGQFEVTGLADGTYNLEETLA
                     PAGFAKLAGNIKFVVNQGSYITGGNIDYVANSNQKDATRVENKKVTIPQTGGIGTILF
                     TIIGLSIMLGAVVIMKRRQSKEA"
     gene            3417..4238
                     /locus_tag="VBC28_001768"
     CDS             3417..4238
                     /locus_tag="VBC28_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEO6815728.1"
                     /translation="MILFLIGLSILLYPVVSRFYYTIESNNQTQDFERAAKKLSQKEI
                     NRRMALAQAYNDSLNNVHLEDPYEKKRIQKGVAEYARMLEVSEKIGTISVPKIGQKLP
                     IFAGSSQEVLSKGAGHLEGTSLPIGGNSTHTVITAHSGIPDKELFSNLKKLKKGDKFY
                     IQNIKETIAYQVDQIKVVTPDNFSDLLVVPGHDYATLLTCTPIMINTHRLLVRGHRIP
                     YKGPIDEKLIKDGHLNTIYRYLFYISLVIIAWLLWLIKRQRQKNRLASVRKGIES"
     gene            4238..5122
                     /gene="srtC4"
                     /locus_tag="VBC28_001769"
     CDS             4238..5122
                     /gene="srtC4"
                     /locus_tag="VBC28_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-2a pilus assembly sortase SrtC4"
                     /protein_id="HEO6815729.1"
                     /translation="MGGKFQKNLKESVVLNRWMNVGLILLFLVGLLITSYPFISNWYY
                     NIKANNQVTNFDNQTQKLNTKEINRRFELAKAYNRTLDPSRLSDPYTEKEKKGIAEYA
                     HMLEIAEMIGYIDIPSIKQKLPIYAGTTSSVLEKGAGHLEGTSLPIGGKSSHTVITAH
                     RGLPKAKLFTDLDKLKKGKIFYIHNIKEVLAYKVDQISVVKPDNFSKLLVVKGKDYAT
                     LLTCTPYSINSHRLLVRGHRIKYVPPVKEKNYLMKELQTHYKLYFLLSILVILILVAL
                     LLYLKRKFKERKRKGNQK"
     gene            5119..6045
                     /gene="pilC"
                     /locus_tag="VBC28_001770"
     CDS             5119..6045
                     /gene="pilC"
                     /locus_tag="VBC28_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-2a pilus subunit PilC"
                     /protein_id="HEO6815730.1"
                     /translation="MKKIRKSLGLLLCCFLGLVQLAFFSVASVNADTPNQLTITQIGL
                     QPNTTEEGISYRLWTVTDNLKVDLLSQMTDSELNQKYKSILTSPTDTNGQTKIALPNG
                     SYFGRAYKADQSVSTIVPFYIELPDDKLSNQLQINPKRKVETGRLKLIKYTKEGKIKK
                     RLSGVIFVLYDNQNQPVRFKNGRFTTDQDGITSLVTDDKGEIEVEGLLPGKYIFREAK
                     ALTGYRISMKDAVVAVVANKTQEVEVENEKETPPPTNPKPSQPLFPQSFLPKTGMIIG
                     GGLTILGCIILGILFIFLRKTKNSKSERNDTV"
BASE COUNT         2264 a          942 c         1124 g         1766 t
ORIGIN      
        1 gccgatacat ctaacaatga aattttatct aaaattcagc aacaatttga aacgatttta
       61 acaaaagaaa actcaattgt taatggaact atcgaagatc ctatgggtga taaaatcaat
      121 ttacagcttg gtaatggaca aacattacag ccaagtgatt atactttaca gggaaatgat
      181 ggaagtgtaa tgaaggatgg tattgcaact ggtgggccta ataatgatgg tggaatactt
      241 aagggggtta aattagaata catcggaaat aaactctatg ttagaggttt gaatttagga
      301 gaaggtcaaa aagtaacact cacatatgat gtgaaactag atgacagttt tataagtaac
      361 aaattctatg acactaatgg tagaacaaca ttgaatccta agtcagagga tcctaataca
      421 cttagagatt ttccaatccc taaaattcgt gatgtgagag aatatcctac aataacgatt
      481 aaaaacgaga agaagttagg tgaaattgaa tttataaaag ttgataaaga taataataag
      541 ttgcttctca aaggagctac gtttgaactt caagaattta atgaagatta taaactttat
      601 ttaccaataa aaaataataa ttcaaaagta gtgacgggag aaaacggcaa aatttcttac
      661 aaagatttga aagatggcaa atatcagtta atagaagcag tttcgccgga ggattatcaa
      721 aaaattacta ataaaccaat tttaactttt gaagtggtta aaggatcgat aaaaaatata
      781 atagctgtta ataaacagat ttctgaatat catgaggaag gtgacaagca tttaattacc
      841 aacacgcata ttccaccaaa aggaattatt cctatgacag gtgggaaagg aattctatct
      901 ttcattttaa taggtggagc tatgatgtct attgcaggtg gaatttatat ttggaaaagg
      961 tataagaaat ctagtgatat gtccatcaaa aaagattaaa aattatgcgc tttagtattc
     1021 ttaattaatt aactaaaatt ccatgacttg aagggagtgg tgctgcggtg tattaaaatc
     1081 cgcatattat taatcatgtt gattaactaa aataagaagg agatagaaat gaaaagaatc
     1141 aacaaatatt ttgcaatgtt ctcggcattg ttactgactt taacgtcatt gctctcagtt
     1201 gcaccagcgt ttgcggatga agcaacaact aatacagtga ctttgcacaa gattttgcaa
     1261 accgaatcaa atcttaacaa aagtaacttc ccaggaacta caggtcttaa cggaaaagac
     1321 tacaaaggtg gagctatttc tgaccttgct ggttactttg gcgagggatc taaagaaatc
     1381 gaaggtgcgt tctttgcttt agctttgaaa gaagataaaa gtggtaaagt gcaatatgtt
     1441 aaggcaaaag aaggtaacaa attaacacca gccttaatta ataaagatgg tactcctgaa
     1501 ataacagtaa atattgatga ggccgtgtct ggattgacac cagagggaga tactggactt
     1561 gttttcaaca ccaaaggatt gaaaggcgag tttaaaattg ttgaagttaa atcaaaatct
     1621 acttacaaca ataatggttc cctcctggct gcttcaaaag cggttccagt taacatcact
     1681 cttccattgg taaatgaaga tggtgttgtt gctgatgccc atgtttatcc aaagaacact
     1741 gaagaaaaac cagaaattga taaaaacttt gctaaaacaa acgatttgac agcattgaca
     1801 gatgttaata gacttttgac agctggcgca aattatggta attatgcacg tgacaaagca
     1861 actgctactg ctgaaatcgg taaagttgtt ccttatgaag ttaaaacaaa aattcacaaa
     1921 ggttctaaat acgaaaactt ggtttggaca gatataatgt caaatggttt gacaatgggt
     1981 tcaactgtta gccttaaagc ttcaggaact acagaaactt ttgctaagga tacagactat
     2041 gaacttagca ttgatgcccg tggtttcaca ttaaaattca cagctgatgg attgggcaaa
     2101 ttggaaaaag cagctaaaac agctgatatt gaatttacat tgacttatag tgctactgtt
     2161 aatggtcaag caattattga taatccagaa tccaatgata tcaaattgtc gtatggtaac
     2221 aaaccaggta aagacttgac tgaacttcct gttacacctt caaagggtga agtaacagtt
     2281 gctaaaactt ggtctgacgg aattgcacct gatggtgtaa acgttgttta cacattgaaa
     2341 gataaagata aaactgttgc ttcagtatca ttgacaaaaa catctaaagg tacaatcgac
     2401 cttggaaatg gtatcaaatt tgaagtatct ggtaacttct cgggtaaatt cactggtcta
     2461 gaaaacaaat catacatgat ctcagaacgt gtttctggtt acggaagtgc aataaatcta
     2521 gaaaatggta aagtaaccat taccaatacc aaagattctg ataacccaac accattgaac
     2581 ccaactgaac caaaagttga aactcatggt aagaaatttg tcaaaactaa tgaacaaggt
     2641 gaccgtttgg ctggtgcaca attcgttgtg aaaaactcag caggtaaata ccttgctctt
     2701 aaagcagatc aatcagaagg tcaaaaaact ttagctgcta agaaaatagc tttagatgaa
     2761 gctatcgctg cttataacaa gttgtctgca acagaccaaa aaggtgaaaa aggaattact
     2821 gcaaaagaac ttatcaaaac taaacaagca gattacgatg cagccttcat tgaggctcgt
     2881 acagcttatg agtggataac agataaggct agagccatta cctacacttc aaacgatcaa
     2941 ggtcaatttg aagttacagg tcttgcagac ggtacttaca accttgaaga aacacttgct
     3001 ccagcaggat ttgctaagtt ggcaggtaat attaagtttg tagttaatca agggtcatac
     3061 ataacaggtg gtaacattga ctacgttgct aacagcaacc aaaaagatgc gacacgtgta
     3121 gaaaataaaa aggtaacaat cccacaaaca ggtggtattg gtacaattct tttcacaatt
     3181 attggtttaa gcattatgct tggagcagta gttatcatga aaagacgcca atcaaaggaa
     3241 gcttaaggct agtctttgat gatgtataag cgcagttaaa gctgtgctta tgatctaagg
     3301 gtatttcagt agaagtactc ttagatcata agcaagagcc attatctagg agatgacgtg
     3361 aagactaaaa aaatcatcaa aaaaacaaaa aaaagaagaa gtcaaatctt ccttttatca
     3421 ttctttttct aataggtcta tctattttat tgtatccagt ggtatcacgt ttttactata
     3481 cgatagaatc taataatcaa acacaggatt ttgagagagc tgctaaaaaa cttagtcaga
     3541 aagaaatcaa tcgacgtatg gctctagcac aagcttataa tgattcttta aataatgtcc
     3601 atcttgaaga tccttatgag aaaaaacgaa ttcaaaaggg ggtagcagag tacgcccgta
     3661 tgttagaggt aagtgaaaaa atcggaacaa tttcagttcc taagataggt caaaaactcc
     3721 ctatatttgc aggttcaagt caagaagttc tatctaaagg agcagggcat ttagaaggta
     3781 cctctcttcc aattgggggc aatagtacac atactgttat aacagcgcat tcaggaattc
     3841 cagataaaga actcttttct aaccttaaaa agttaaaaaa aggagataag ttttatattc
     3901 aaaacataaa agaaacgata gcatatcaag tagatcagat aaaagtcgtt acacccgata
     3961 acttttcaga tttgttggtt gttcctggac atgattatgc aaccttattg acttgcaccc
     4021 cgattatgat caatacacac agacttttag taaggggaca tcgtatccct tataaaggtc
     4081 ctattgatga aaaattaata aaagacggtc atttaaacac gatttataga tatctattct
     4141 atatatcttt agttattatt gcttggttac tttggttaat aaaacgtcaa cgtcaaaaaa
     4201 atcgtttagc aagtgttaga aaaggaattg aatcataatg ggaggaaaat ttcagaaaaa
     4261 ccttaaggaa tcggtcgttt taaatcgatg gatgaatgta ggcttgatac tattgttctt
     4321 agttggtctt ttgataacct catatccttt tatttcaaat tggtactata atattaaagc
     4381 taataatcaa gtaactaact ttgataatca aacccaaaaa ttaaatacta aagagattaa
     4441 tagacgattt gagttagcaa aagcttataa tagaacactg gacccaagcc gcctatcaga
     4501 tccctatact gaaaaagaaa aaaaaggtat tgctgaatac gcccacatgc ttgagattgc
     4561 tgaaatgatt ggatatattg atataccgtc tatcaagcaa aaattaccta tctatgcggg
     4621 gactaccagt agtgttcttg aaaaaggagc aggacacctt gaaggaacct ccttgccaat
     4681 tggtggaaaa agttcacata ctgttatcac agctcatcgc ggcttaccta aagctaagtt
     4741 atttacagat ttagataaac ttaaaaaagg aaaaattttt tatattcata atatcaaaga
     4801 agttttagcc tataaggttg atcaaataag tgttgtaaag ccagataatt tttctaaatt
     4861 attggttgtt aaaggtaagg attatgcgac tttgctaaca tgtacacctt attcgattaa
     4921 ttcacatcgt ttactagtta gagggcatcg aatcaagtat gtacctcctg ttaaagaaaa
     4981 gaactattta atgaaagaat tgcaaacaca ctataaactt tatttcctct tatcaatcct
     5041 agttattctt atattagtcg ctttactatt atatttaaaa cgaaaattta aagagagaaa
     5101 gagaaaggga aatcaaaaat gaaaaagatt agaaaaagtt taggacttct actatgttgc
     5161 tttttaggat tggtacaatt agcgtttttt tcggtagcca gtgtaaatgc tgatacccct
     5221 aatcaactaa caatcacaca gataggactt cagccaaata ctacagagga ggggatttct
     5281 tatcgtttat ggactgtgac tgacaactta aaagttgatt tattgagcca aatgacagat
     5341 agcgaattga accagaagta taagagtatc ttgacttctc ctactgatac taatggtcag
     5401 acaaagatag cactcccaaa tggttcgtac tttggtcgtg cttataaagc tgatcaaagc
     5461 gtttcaacaa tagtaccttt ttatattgaa ttaccagatg ataagttatc aaatcaatta
     5521 cagataaatc ctaagcgaaa agttgaaaca ggccgattaa aacttattaa atatacaaaa
     5581 gaaggaaaga taaagaaaag gctatccgga gtaatatttg tattatacga taaccagaat
     5641 cagccagttc gctttaaaaa tggacgattt acgaccgatc aagatgggat tacttcatta
     5701 gtaactgatg ataagggaga aattgaggtt gaaggtttat tacctggtaa gtatattttt
     5761 cgagaagcaa aagcactaac tggttaccgt atatctatga aggatgctgt agttgctgta
     5821 gttgctaata aaacacagga agtagaggta gaaaacgaaa aagaaactcc tccaccaaca
     5881 aatcctaaac catcacaacc gctttttcca caatcatttc ttcctaaaac aggaatgatt
     5941 attggtggag gactgacaat tcttggttgt attattttgg gaattttgtt tatcttttta
     6001 agaaaaacta aaaatagcaa atctgaaaga aacgatacag tatagtaatt tggtattaat
     6061 atgattttta atcactacta ctaattagta attaaa
//
LOCUS       DATRMA010000090         6047 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.091, whole genome shotgun sequence.
ACCESSION   DATRMA010000090 DATRMA010000000
VERSION     DATRMA010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6047
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.091"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            <1..74
                     /locus_tag="VBC28_001771"
     CDS             <1..74
                     /locus_tag="VBC28_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HEO6815731.1"
                     /translation="VDRSELSASKYCLGRAVSKHYRF"
     gene            126..803
                     /locus_tag="VBC28_001772"
     CDS             126..803
                     /locus_tag="VBC28_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HEO6815732.1"
                     /translation="MKDLLRNSLEQSGNLSFQDMILHILVAALLSVVIYVSYAYTHSG
                     TAYSKKFNVSLMTLTVLTATVMTVIGNNVALSLGMVGALSVVRFRTAIKDSRDTVYIF
                     WTIVVGICCGVGDYVVAALGSSVIFILLWVMGRVKNENRMLLIVKCDRTLEVDLEGIF
                     FQYFDGKAVQRVKNSTTNTIEMIFEISRKDYDKQLHVDNQLTEKVYQLGNIDYFNIVS
                     QSDEING"
     gene            796..2241
                     /locus_tag="VBC28_001773"
     CDS             796..2241
                     /locus_tag="VBC28_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HEO6815733.1"
                     /translation="MGRLMRGKVIYGTTLIGLFLFLFFYFWIPKHHIERIHHHRIKQV
                     DAKSDLTGFKTHLPIISIDTKQQVIPLVTKEGGKYVKARDNINVDIELRDSPSRSHHL
                     SEKPRIRTKGLISYRGNSSRYFDKKSLKVKFVTNKLKEKKHRLAGMPKESEWVLHGPF
                     LDRTLLRNYLSYNIAGEIMSYAPNVRYCELFVNGEYQGVYLAVENIEQGEQRVPIEKS
                     DKKLHKTPYIVAWDREHKAKQKLDNYVHYTHQSGISALDVKYPGKQRLTSKQLEFINK
                     DINHIEKVLYSYDFSQYPKYIDRESFANYFVINEFFKNVDAGKFSTYLYKDLRDRAKL
                     VVWDFNNAFDNQIEGRVDEADFTLTDAPWFNMLIKDKAFIDLVVHRYKELRKGVLATE
                     YLSNYIDETRHFLGPAIDRNYKKWGYVFDLKNTDPRNYLIPTERNVTSYHKSVEQLKD
                     FIKKRGRWMDRNIETLYQYSAPSKNTNTLLE"
     gene            2248..4407
                     /locus_tag="VBC28_001774"
     CDS             2248..4407
                     /locus_tag="VBC28_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000760424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6815734.1"
                     /translation="MKKYLSIVALLTILFSSIYYLYYFDGSLYLPKGLLKENTRTNFV
                     VKGDTVLHKPTNKPFVVKGVDVESSLAGYHHNDFPITQKTYREWFHLISNMGANTVRV
                     KVPMNVAFYDALYHHNKASKRPLYLLQGIRIDSYRNNASITAFNDNYRGYLKREAKGV
                     VDILHGRKQVWNTDLGSRHYHYDLSPWVLGYVVGDDWNSGTVAYTNHQEKKTQYKGRY
                     FKTSVAANPFEVMLAQVMDELTHYETAKYGWQHLISFSNSPTTDPFHYRKPFEAQAPK
                     YVQLNVENIQANSNVKAGMFAAYKAIDFHPRYKDYLLFDKENISKEDRQKIKELSLSQ
                     GYVKLLNAYHKIPVLVTGYGYSTARGIAQKEIDKRPLPINEKEQGQRLLEDYESFISS
                     GSFGATINAWQDDWNARAWNTSFATNKHSQFLWGDAQVFNQGYGLLGFKNAKHHYQVD
                     GKRGKGEWKHPLMTSATGDDLYASSDESYLYLAIKTKPEKLKEKRLLPIDITPKSGSR
                     KMNGSKVTFSKSSDFVLSIDPNGKSELFVQERYNALKANYLRQLNGKDFYAFPPKKNS
                     SNFEQINMVLRNTKIVEDMEKVKATERFLPTHPTGLLKTGTTDRHQKTFDSQTDISFG
                     KDFIEVRIPWQLLNFSDPSSQKIHDDYFKHYGVKELEIESIALGLGANSKENTLIKMA
                     DYRLKNWERPDTKTFLKDSYYSIKKEWSKERERTYGT"
     gene            4547..5959
                     /locus_tag="VBC28_001775"
     CDS             4547..5959
                     /locus_tag="VBC28_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEO6815735.1"
                     /translation="MALFFTFLKVSQVIFLVLLIAYASFLVISLVIGGVHLYEKNERK
                     KSLTRNKEKIPISIIVPAYNESTTIVSSIDSLLHLDYEAYEIIVVDDGSSDNTSDVLK
                     EEFALMKISNTIDSIIATQTCKDVFQRQVGKVKLTLIVKENGGKGDALNMGINAANYD
                     YFLCLDADSMLQVDSLSQISKSIQVDPTVIAVGGLVQVAQGVKIEQGKVASYRLPWRI
                     IPCAQALEYDSSFLGARIFLDYLRANLIISGAFGLFKKDLVKAVGGYDTQTLGEDMEL
                     VMKLHFFCRNNNIPYRICYETDAVCWSQAPTNLGDLRKQRRRWYLGLYQCLKKYKSIF
                     ANYRFGAVGSISYIYYILFELLTPFIECFGIVIIFLSLLFNQLNIPFFISLVSLYIFY
                     CVLITLSSFLHRIYSQQLVIGILDIVKVFYIAVFRYLILHPVLTFVKVASVIGYKNKK
                     MVWGHITREKQMKDFETSVT"
BASE COUNT         2048 a          873 c         1168 g         1958 t
ORIGIN      
        1 cggtagatag aagtgaactt tcagcgagta agtattgttt aggaagagct gttagtaaac
       61 attatcgttt ttagtgaaaa agtatatacg ttaattatta ttaaatgtga gaggataaca
      121 aaaaaatgaa agacctatta cgaaatagtc tagagcaaag tggaaattta agttttcaag
      181 atatgatttt acatattctt gtagcagctt tattgagtgt agttatttat gtttcctatg
      241 cttatacgca tagtggaact gcctatagta aaaagtttaa tgtttcatta atgacattga
      301 cggtcttgac tgcaacagta atgaccgtta ttggtaataa tgtagccttg tcattgggta
      361 tggtcggtgc cttgtcagtt gttcgtttta ggacagccat aaaagattca agagatacag
      421 tttatatttt ttggaccata gttgttggta tctgttgtgg tgtcggtgac tatgtggtag
      481 ctgcattagg aagtagcgtt atctttatct tattatgggt tatgggacgt gttaaaaacg
      541 agaatcgtat gttattgatt gtgaagtgcg atagaacact agaagttgat ttagaaggaa
      601 ttttcttcca atattttgac ggaaaagctg ttcagcgtgt taaaaattca acaactaata
      661 ctattgaaat gattttcgaa atctctagaa aagattacga taagcaactc catgtagata
      721 atcagttaac tgaaaaagtg taccaattgg gaaatattga ttatttcaac attgttagcc
      781 aaagcgacga aatcaatggg tagacttatg agagggaagg ttatttacgg cacaaccctt
      841 ataggtcttt ttctattctt atttttctat ttttggattc ctaagcatca catcgagaga
      901 atacatcatc atcgtataaa gcaggtagat gcgaagagtg atttaacagg atttaaaacc
      961 catttgccca ttatcagcat tgatacaaag caacaagtta ttcctcttgt tacaaaagaa
     1021 ggcggaaaat atgtcaaagc tagggataat attaatgttg atatcgaatt acgggattct
     1081 ccaagtagat cacatcattt atcagaaaag ccgagaatta ggacaaaagg gttaatatca
     1141 tatagaggaa attcctctcg ttactttgat aagaagtcat tgaaagttaa gtttgttact
     1201 aataagttaa aggaaaagaa gcatcgatta gcaggaatgc ctaaagaatc ggagtgggta
     1261 ttgcatggtc cctttctaga cagaacatta ttaagaaatt atctgagtta taatattgct
     1321 ggtgagatta tgtcctatgc cccaaacgtt cgctactgtg agttatttgt caatggtgag
     1381 tatcagggag tatatctagc agttgagaat atcgaacaag gtgagcaacg agttcccatt
     1441 gaaaaaagtg ataaaaagtt gcataaaaca ccttatatcg ttgcttggga tcgtgaacat
     1501 aaggcaaagc aaaagttaga taactatgta cattatacac accagtctgg tatctcagct
     1561 cttgatgtga aatatccagg taaacaaaga ttgacatcaa aacaattaga gtttattaat
     1621 aaggacatca atcatattga aaaagtcctt tattcttatg atttttcaca atatcctaaa
     1681 tatattgata gagaatcatt tgcaaattat tttgtcatca atgaattttt taaaaatgtg
     1741 gatgcgggta aattttcaac ctatctatat aaagatcttc gtgatagagc aaaactagta
     1801 gtttgggatt ttaataatgc ttttgataat caaattgaag gacgtgttga tgaggcagat
     1861 tttaccttga cggatgcacc gtggttcaat atgctcatta aggacaaagc atttatagac
     1921 ctggttgttc atcgctataa agaattacga aagggagttc tagcgacaga atatttatct
     1981 aattacatag acgagacaag gcatttttta ggtccagcta ttgatcgaaa ttataaaaaa
     2041 tggggctatg tctttgattt aaagaatact gatccaagga attatttgat tcctactgaa
     2101 cgcaatgtga caagttatca caaatcagta gaacaattaa aagattttat caaaaagcgt
     2161 ggcagatgga tggatagaaa tattgagacc ttgtatcaat atagtgcacc atcgaaaaat
     2221 actaatactt tactggagta gaacattatg aaaaaatatc taagtattgt agccctttta
     2281 actattttgt ttagctctat ttattatttg tattactttg atggtagttt gtatttacca
     2341 aagggcttat taaaagaaaa tacaagaact aactttgttg ttaaaggtga tactgtactt
     2401 cacaagccca ccaataaacc ttttgttgtt aaaggagtag acgttgagtc ttccttagcg
     2461 ggttatcatc acaacgattt tcctattact caaaaaacgt atcgtgaatg gttccattta
     2521 atttccaaca tgggggcaaa tactgtaaga gtcaaggtac cgatgaatgt tgcattttac
     2581 gatgccttat atcaccacaa caaagcatca aagaggccac tgtatttgtt gcaaggaata
     2641 cgtatagatt cttatcgcaa taatgcttct ataacagctt ttaatgataa ttataggggg
     2701 tatttaaaac gagaagcaaa aggcgttgtg gatattctcc atgggcgtaa gcaagtatgg
     2761 aatactgatt tgggtagccg tcattatcat tatgatctta gtccttgggt acttggttat
     2821 gtcgtagggg atgattggaa tagtggtact gtcgcttata ctaatcatca agagaaaaaa
     2881 acgcaatata aaggacgtta ttttaaaact tctgtggcag ctaatccatt tgaggtcatg
     2941 ctagctcaag taatggatga attgacacat tatgagacag ctaaatatgg ttggcaacat
     3001 ttgattagtt tttcaaactc accaacaaca gacccttttc attatcgaaa accatttgag
     3061 gcacaggctc ctaaatacgt acaactaaat gtagaaaata ttcaagctaa ttcaaatgtt
     3121 aaagcaggta tgtttgcagc atataaagct attgatttcc atcctcgata caaggattat
     3181 ctattatttg ataaagagaa tatcagtaaa gaagatagac aaaagattaa agaactttct
     3241 ttgtcacagg gatacgttaa actgctaaat gcttatcaca aaatccctgt tctagtcacg
     3301 ggttatggct attcgacagc gagaggtatt gcccaaaaag aaattgataa acgtcctctg
     3361 ccgattaatg aaaaagaaca aggtcagcgt ttactagaag attatgaatc ttttatatca
     3421 tccggtagtt ttggagcgac tatcaatgca tggcaagacg attggaatgc aagggcgtgg
     3481 aatacatctt tcgccacaaa taaacatagt caattcctat ggggggatgc acaagtattt
     3541 aatcaaggtt atggtttatt aggctttaaa aacgcaaaac atcattatca agttgatggt
     3601 aaaagaggca aaggagagtg gaaacatcct ctgatgacta gtgcaacagg agatgactta
     3661 tatgctagca gtgatgaaag ctatctctac cttgcgatta aaacaaaacc tgaaaaacta
     3721 aaagaaaaac gattattacc aatagatatt acaccaaaat ctggtagtag aaaaatgaat
     3781 ggtagtaagg tcacattttc taaatctagt gactttgtat tgtctattga tccaaatggc
     3841 aagtctgaat tatttgtcca agagcgctat aatgccttaa aagcgaacta tcttcgacag
     3901 cttaacggta aagattttta tgctttccca ccaaagaaga acagtagtaa ttttgagcag
     3961 ataaatatgg tattgagaaa tacaaagatt gttgaagaca tggaaaaagt aaaagcaaca
     4021 gagaggttct taccaactca tcctactggt cttctcaaaa caggaacaac tgataggcac
     4081 caaaaaacat ttgattcaca aacagatatt tcgtttggaa aggactttat agaggtcaga
     4141 attccgtggc agttgttgaa tttttctgat ccatcatctc aaaaaattca cgatgattac
     4201 tttaaacatt atggtgtgaa ggagttagaa attgagagca ttgctttagg attaggtgct
     4261 aatagcaaag aaaacacact gataaagatg gcagattatc gtttgaaaaa ttgggagaga
     4321 cccgatacca aaaccttttt aaaagactcc tattatagta ttaagaaaga atggtctaaa
     4381 gaaagagaga gaacatatgg tacataatat tttatatttt tacattttga tttgcttagc
     4441 aattattgtt tttaacattc actatatctt aagacaacga ttagataagc ttagttggct
     4501 tcgtattcgg aaaaaggata taaatatcaa ataaagggga ttttctatgg cattattttt
     4561 taccttccta aaggtatctc aagtcatttt cttagtatta ttgattgcct atgccagctt
     4621 tttagtgatt tcattagtca ttggaggtgt tcatctctat gaaaaaaatg agcgcaaaaa
     4681 aagtttaact agaaataaag aaaaaattcc aatttcaatt attgtaccag cctataatga
     4741 atcaacaacc attgtgagtt cgattgattc gttattgcac cttgattatg aagcgtatga
     4801 aattatcgtt gtggatgacg gttccagtga caatacttca gatgttctca aggaagagtt
     4861 tgcattaatg aaaatctcga atacgattga ttcaattatt gctactcaaa cttgtaaaga
     4921 tgtctttcag agacaggttg gtaaggttaa actgacactc attgttaaag aaaatggtgg
     4981 caaaggggat gccttaaata tggggatcaa tgctgctaat tacgattatt ttctatgtct
     5041 agatgcagat tctatgttgc aggtagattc tttgagtcaa attagcaaat ccatacaggt
     5101 agatccgacg gttatagcag tgggagggct cgtccaagtt gcccaaggtg ttaagattga
     5161 gcaggggaaa gtagcatctt atcgtttgcc ttggagaatt attccttgtg cacaagcact
     5221 tgagtatgat tcatcatttt taggagctcg aatcttttta gactatttaa gagctaattt
     5281 aattatttcg ggggctttcg gtctttttaa gaaagacctt gttaaagcgg tgggaggata
     5341 tgacacccag actctaggag aagatatgga gttggtgatg aaattacatt tcttctgtcg
     5401 taataataat attccttatc gtatttgtta tgaaacagat gctgtttgtt ggagtcaagc
     5461 accaacgaat ttaggggatt tacgtaaaca gagacgccga tggtatcttg gcttgtacca
     5521 atgtttaaaa aaatataaaa gtatttttgc caattatcgc tttggagctg taggatcaat
     5581 ttcttatatt tactatattt tatttgaatt actgacccct tttatagaat gttttggtat
     5641 tgtcattata tttttgtcct tattatttaa tcaattaaac atcccattct ttatatcatt
     5701 ggtgagcctt tatatctttt actgtgtttt aattacatta agctctttct tacaccgtat
     5761 ttattctcag caactagtaa taggaatttt agatattgtt aaagtatttt atattgctgt
     5821 ttttaggtat cttatcctac atcctgtatt aacttttgtt aaggttgcaa gtgttattgg
     5881 ctataaaaat aaaaaaatgg tttgggggca tattacgaga gagaaacaaa tgaaggattt
     5941 tgaaacaagt gttacttaga agttatagcg attcatatat ataaaataat cactaaaaga
     6001 gcgtaattat ctacgctctt ttagtgatta tcaataattg gcctaat
//
LOCUS       DATRMA010000091         5931 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.092, whole genome shotgun sequence.
ACCESSION   DATRMA010000091 DATRMA010000000
VERSION     DATRMA010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5931
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.092"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            complement(<1..593)
                     /locus_tag="VBC28_001776"
     CDS             complement(<1..593)
                     /locus_tag="VBC28_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR family transcriptional regulator"
                     /protein_id="HEO6815736.1"
                     /translation="MSNHSRRQQKKHSHTPLRVINLFLLVIFILLSVVSLFLMYRHHF
                     LAFRHLNVIYGVVIVLIILASLFLCIKNKARIFTTIILVLASIFVATTLYGFKSTIDL
                     TNNLNKTASYSEIEMSVIVPKDSKITNIEAVSKLAAPVKNDTSNITDLIEHIKSEKGI
                     SITPQKTDSYQDAYNRIKNGDSQAMVLNNAYVSLIEL"
     gene            782..1705
                     /locus_tag="VBC28_001777"
     CDS             782..1705
                     /locus_tag="VBC28_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEO6815737.1"
                     /translation="MRIQQLQYVIKIVETGSMNEAAKQLYITQPSLSNAVRNLETEMG
                     IQIFIRNPKGITLTKDGMEFLSYARQILEQTALLEERYKGDNTSRELFSVSSQHYAFV
                     VNAFVALFNGTDMTQYELFLRETRTWEIIDDVKNFRSEIGVLFLNSYNRDVLTKLFDD
                     NSLIATTLFTTTPHIFVSKSNPLANRKKLSMKDLEDYPYLSYDQGLHNSFYFSEEMMS
                     QIPHPKSIVVSDRATLFNLMIGLDGYTVATGILNSKLNGDEIVAIPLDVDDVIDIVYI
                     RHDKANLSKMGQKFIDYLLEEVSFNHKNKQS"
     gene            complement(1730..2497)
                     /locus_tag="VBC28_001778"
     CDS             complement(1730..2497)
                     /locus_tag="VBC28_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000129264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR family transcriptional regulator"
                     /protein_id="HEO6815738.1"
                     /translation="MTDFSTALKVLVDQYSYHNAFLLLQKHGPLNSDLLFLLEMMKER
                     RELNIDFLFAHQEQVVILQEKYNIKLLHNSYDLELLANYIMDLEAKVKNGLIIDFVRS
                     VSPILYRLFMILLAQEVPHLHDYIHNARDDHYDTWKFKELKESNHPVLLAFSERWHDS
                     RLTSKSLAECLQLTDLDEEVKSTIIQLRQFEKSVRNPLAHLIKPFDEQELYRTTQFSS
                     QAFLDQIIFLAKVIGVEYDTVNFHYDTVNKLIIKILE"
     gene            complement(2506..3216)
                     /gene="deoD"
                     /locus_tag="VBC28_001779"
     CDS             complement(2506..3216)
                     /gene="deoD"
                     /locus_tag="VBC28_001779"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEO6815739.1"
                     /translation="MSIHIEAKQGEIADKILLPGDPLRAKFIAENFLEDAVCFNTVRN
                     MFGYTGTYKGHRVSVMGTGMGMPSISIYARELIVDYGVKTLIRVGTAGAINPDIHVRE
                     LVLAQAAATNSNIIRNDWPEFDFPQIADFKLLDKAYHIAKEMDITTHVGSVLSSDVFY
                     SNQPDRNMALGKLGVHAIEMEAAALYYLAAQHNVNALAMMTISDNLNNPEEDTSAEER
                     QTTFTDMMKVGLETLISE"
     gene            complement(3200..4456)
                     /locus_tag="VBC28_001780"
     CDS             complement(3200..4456)
                     /locus_tag="VBC28_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEO6815740.1"
                     /translation="MVSRLYYAVKFMIAVLFMTVMAGVGAILMHYVLMFTEWLAFGDS
                     RENTLSLLNSVTPIKRVLSLTLASFLASLSWYYLQIKPKQITSIKQQVVFKDSSLKKS
                     PYWLHIGHAFLQLIYVGAGGPIGKEGAPREFGAINAGKISDLLALKVLDKRLLIISGA
                     AAGLSAVYQVPLASVFFAFETLALGISLKNIVTLLASTFGAASIAQLVISTAPLYRIS
                     KMSLNSQSLAFMFLIVLCVTPIAISFRYLNQKVTERRIKNIKILLSLPVVSLIVSVLS
                     IVYPQILGNGNALVQEVFKGTTVSLITILVVLKMIATLSTLYAGAYGGILTPSFSIGA
                     CLGFLLASISIPLLPHISIVTSMLVGAAIFLAITMRAPLTAVGLVISFTGQSVITIVP
                     LTIAVLFATAYDYFIRKMRSLYVNPY"
     gene            complement(4458..5267)
                     /locus_tag="VBC28_001781"
     CDS             complement(4458..5267)
                     /locus_tag="VBC28_001781"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEO6815741.1"
                     /translation="MTLLEKINETRDFLQAKGVTAPEFGLILGSGLGELAEEIENPIV
                     VDYADIPNWGQSTVVGHAGKLVYGDLSGRKVLALQGRFHFYEGNTMEVVTFPVRIMRA
                     LACHSVLVTNAAGGIGYGPGTLMLIKDHINMIGTNPLIGENLEEFGPRFPDMSDAYTA
                     TYRQKAHQIAEKQNIKLEEGVYLGVSGPTYETPAEIRAFQTMGAQAVGMSTVPEVIVA
                     AHSGLKVLGISAITNFAAGFQSELNHEEVVEVTQRIKEDFKGLVKSLVAEL"
     gene            complement(5306..5713)
                     /gene="arsC"
                     /locus_tag="VBC28_001782"
     CDS             complement(5306..5713)
                     /gene="arsC"
                     /locus_tag="VBC28_001782"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416958.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HEO6815742.1"
                     /translation="MEKVRIYHNPNCGTSRNVLAIIRHCGIEPEIIYYLKTPPSRMEL
                     VELLLEMKLSARELLRTDVPAYEKFNLESSSVTDEEMIDAMIQDPILINRPIVVTSKG
                     AKLCRPCEEILTILPVKMEKDFVKEDGQIIQSL"
     gene            complement(5764..>5931)
                     /locus_tag="VBC28_001783"
     CDS             complement(5764..>5931)
                     /locus_tag="VBC28_001783"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019318858.1"
                     /note="catalyzes the transfer of phosphate between the C1
                     and C5 carbons of pentose; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEO6815743.1"
                     /translation="TDHTREYIPLLAYSPSFTGNGLIPVGHFADISATVADNFGVDTA
                     MIGESFLQDLV"
BASE COUNT         1964 a         1107 c          972 g         1888 t
ORIGIN      
        1 ctaagttcaa ttaagctaac ataagcatta tttaaaacca tagcctgact atcaccattt
       61 ttaattctat tgtatgcatc ctggtaagaa tctgtttttt gtggtgtaat agagattcct
      121 ttttctgatt ttatatgttc tatcaaatca gtaatatttg aagtatcgtt tttaactggt
      181 gcggctaatt tgctgacagc ttctatattg gttattttag aatcttttgg tacaattaca
      241 ctcatctcaa tttcagagta tgaagcagtt ttatttagat tatttgtcaa atcaatggtt
      301 gacttaaatc catataaagt agtagcaacg aaaatagaag ctaatactaa aattatagtt
      361 gtaaaaattc tagctttatt cttaatacaa agaaataaac ttgctaaaat gattaaaaca
      421 attacaactc cataaatgac gttcaggtgt ctaaatgcca aaaaatggtg acgatacata
      481 agaaataatg agactacact taacaaaata aaaatcacca aaagaaataa attaatcacc
      541 cgtagaggtg tatgtgagtg tttcttttgt tgacggcgcg aatgattaga cattgtaatt
      601 ctccaatact taaaaaacct taattttact tggatattat atcatttttc aatacaatat
      661 tcaacgaaag taatattgga ataatctgat tatattagct aaataacatt ttcaattgat
      721 ctatattgat aatttagctg atctaattga atttatttac attctagttt aaactagagt
      781 tatgagaatt caacaattac aatatgttat taagattgta gaaactggta gcatgaatga
      841 agccgctaaa caactctaca ttacccaacc ttcactttca aacgctgtta ggaaccttga
      901 gactgagatg gggatccaaa tttttattcg aaatccaaaa ggaataacgt taacaaaaga
      961 tggtatggaa tttctgtcat atgcgcgaca aattctagaa caaacagcac ttcttgaaga
     1021 gcgttataag ggtgataata caagtcgtga gctgttcagc gtttcttcac aacactatgc
     1081 atttgtggtt aacgcttttg tagctctctt taatggcaca gacatgaccc aatacgaact
     1141 ttttttacgt gaaactagga cttgggaaat tattgatgat gttaaaaatt tccgttcaga
     1201 gattggtgtt ctgtttctta atagctataa tagagatgta ttaaccaaat tattcgacga
     1261 taattcctta attgccacaa ctctttttac tactacacct catattttcg tgagcaaatc
     1321 caatccctta gctaatagaa aaaaattaag tatgaaagat ttagaagatt atccttatct
     1381 gagttatgat caaggcctgc ataattcttt ctacttttct gaggaaatga tgtctcaaat
     1441 tcctcaccca aaatccattg ttgtcagtga tagagcaact ctgtttaatt taatgattgg
     1501 attagatggt tatacagtgg caactggtat cctcaactca aaattgaacg gtgacgaaat
     1561 tgtcgcaatc cctcttgacg tggatgatgt tattgatatt gtctatatcc gacacgacaa
     1621 agcaaacctt tcaaaaatgg gacaaaaatt tattgactat ttattagagg aagtttcgtt
     1681 taatcataaa aataaacagt cctgagattg tctcagaact gtttttaaat tactcaagta
     1741 tctttataat aagcttgtta accgtatcgt agtgaaaatt aacagtatca tactcaacac
     1801 caattacctt tgccaagaag ataatctggt ctaaaaatgc ttgagaagaa aattgagttg
     1861 tgcgatatag ttcttgctca tcaaaaggtt taatcaggtg agccaaagga tttctgactg
     1921 atttttcgaa ctgtcttaat tgaatgatgg tcgatttcac ttcttcatca aggtcggtta
     1981 attgtaaaca ttctgcaagg cttttagaag tcaagcgact atcgtgccac ctttcagaga
     2041 atgccaaaag gactggatgg tttgactcct ttaattcttt aaacttccaa gtatcgtagt
     2101 gatcatctct tgcattatgg atgtaatcat gcaagtgagg tacttcttgt gcaagtaaaa
     2161 tcataaatag acgatataaa ataggactaa cactacggac aaaatctatg attaatccat
     2221 tttttacttt tgcttctaaa tccatgatgt aatttgccag caactctaaa tcatacgagt
     2281 tatgcaaaag ctttatattg tatttttctt gtaatataac aacttgttct tgatgagcaa
     2341 aaagaaaatc aatgtttaac tctcgacgct ctttcatcat ttccaataaa aataataaat
     2401 ctgagtttaa aggaccatgt ttttgtaata ataagaaagc attatgataa gaatactgat
     2461 ccactaatac tttaagagca gttgaaaaat ctgtcatttc tatcctcatt cagaaataag
     2521 tgtttcgagc ccaactttca tcatatcagt aaatgttgtt tgacgctctt ctgcacttgt
     2581 atcttcttct ggattattca agttatctga aatcgtcatc atagcaagag cattaacatt
     2641 gtgttgagct gcaagatagt agagagctgc tgcttccatc tcaatagcat gaacacctag
     2701 tttacccaag gccatattac gatctggttg gtttgaataa aacacgtctg aagataatac
     2761 actgcctaca tgagttgtaa tatccatttc ctttgcgatg tgataagctt tatccaaaag
     2821 tttaaaatca gcaatttgcg gaaaatcaaa ttcaggccaa tcattacgga taatattaga
     2881 atttgttgct gctgcttgtg ctaaaacaag ttcacgaaca tggatatcag gattaatagc
     2941 acctgctgtt cctacgcgaa tcaatgtttt aaccccataa tcaacaatta gttcgcgagc
     3001 ataaattgaa attgaaggca tacccatacc agttcccata acagaaacac gatgtccttt
     3061 ataggtacct gtataaccaa acatattgcg aactgtatta aaacacaccg catcttctaa
     3121 aaaattctcg gcaataaatt tcgcacggag tggatctcct ggaagtaaaa ttttatctgc
     3181 aatttcacct tgttttgctt caatatggat tgacatataa actcctcatt tttcttataa
     3241 aataatcata ggcagtagca aaaagtactg ctatagttaa gggaacaata gttataacgg
     3301 attgaccagt aaaagatatg actaaaccga cagctgtcag tggcgctctc attgtaatag
     3361 ctaaaaaaat agctgcacca accaacatac tggttactat cgatatgtgg ggaagtaaag
     3421 gaatagaaat actagctaac aaaaaaccta aacaagcacc aatggaaaaa gatggtgtca
     3481 gtataccgcc ataagcacca gcgtaaagag tggataatgt tgctatcatc ttgagaacaa
     3541 ctaaaatagt gattaagcta actgtggtcc ctttaaagac ctcttgaact agggcattac
     3601 catttcctaa aatctgggga tagactattg ataaaacaga tactatcaat gacactactg
     3661 gcaaacttaa caatattttt atatttttaa tacgtctctc tgtcactttt tgatttaaat
     3721 atctaaagct aatcgcaatc ggagttacac ataaaacgat caagaacata aaagctaatg
     3781 actgtgaatt cagactcatc tttgaaatac gataaagagg ggctgtcgaa ataactaatt
     3841 gagcaatact agctgcacca aaagtagagg ctaacaatgt cacaatattt ttcagtgaga
     3901 ttcctaaagc taaagtttca aaagcaaaga atacactagc taagggtact tgataaactg
     3961 ctgacagacc tgcagctgca ccactaatga ttaataaacg tttatccagc actttaagcg
     4021 ctaataaatc cgatattttc ccagcattta tggctccaaa ctctcgtggc gctccttctt
     4081 ttccaatagg accaccagct cctacataaa tcaattgcaa aaatgcgtga cctatatgta
     4141 gccagtaagg tgattttttt agagaagaat ccttaaaaac aacctgttgt ttaatactag
     4201 ttatttgctt tggtttgatt tgtaagtaat accaactaag ggatgctaaa aaggaggcta
     4261 aagtaagtga taacactctt ttaattggtg tgacactatt taacaaagac agagtatttt
     4321 ctctactatc accaaatgct aaccactctg taaacattaa tacatagtgc attaagatag
     4381 ctccaactcc tgccataact gtcataaata gaacggctat cataaacttg acagcatagt
     4441 ataatcgaga aaccatttta aagttcagca actaatgatt taactaatcc cttgaaatct
     4501 tctttaatac gctgagtaac ttcaacgacc tcctcatgat tgagctcaga ttggaagcca
     4561 gcggcaaagt tagtaattgc tgaaattcct aacactttaa gccctgagtg agctgcaacg
     4621 atcacctctg gaaccgtgga catacctacc gcctgtgcgc ccattgtttg gaatgcacga
     4681 atttctgcag gtgtttcata agtgggtcct gatacaccca agtacacacc ttcttcgagt
     4741 ttgatattct gtttttcagc aatttggtga gctttttgtc gatatgttgc tgtataagca
     4801 tccgacatgt ctgggaaacg tggtccaaat tcttcaaggt tctcacctat gagagggtta
     4861 gtcccaatca tattgatgtg gtctttgatc agcattaaag ttcctggtcc gtatccaatc
     4921 ccacccgctg cattagtcac aagcacactg tggcaagcca atgctctcat gatacgtact
     4981 gggaaagtaa cgacttccat tgtatttcct tcataaaagt gaaaacgacc ttgaagcgct
     5041 aataccttac ggcctgataa atccccatac actaattttc cagcatgacc aactactgtt
     5101 gactgtcccc aatttgggat gtctgcataa tccacaacaa tgggattttc gatttcttca
     5161 gccaattctc ctaaaccaga gcctaaaata aggccaaatt ctggtgctgt gacgcctttt
     5221 gcttgcaaaa agtctctagt ctcattaatt ttttctaata atgtcattta cttacctcct
     5281 aaaccattat tcactataca atatgctata aactttgaat aatctgtcca tcttctttaa
     5341 caaaatcttt ttccatcttt acaggtaaaa tggttaaaat ttcttcacag ggtcggcaga
     5401 gttttgcacc tttactcgta acaactatgg gacgattgat aagaatagga tcttgtatca
     5461 tcgcatcaat catttcttca tctgtaacgc tagaagattc caaattgaat ttctcataag
     5521 caggaacatc tgttcttaga agttccctcg cagaaagttt catttctaac aacagttcaa
     5581 ccagttccat cctgctaggt ggtgttttca gataataaat aatttctggt tcaataccac
     5641 aatgacgaat aatggcaagt acgttcctcg atgtcccaca atttgggtta tggtaaatcc
     5701 taactttttc catcatcttc ctctcaattt ttaatgctac aagtgtctta aggaaataaa
     5761 aatttatacc aagtcctgca agaagctctc tccaatcatc gcagtatcaa caccaaagtt
     5821 atccgcaact gttgctgaaa tatctgcgaa atgccctact gggataagtc cgtttcctgt
     5881 gaaggatggg ctataggcta agagaggaat gtattctctt gtatggtctg t
//
LOCUS       DATRMA010000092         5693 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.093, whole genome shotgun sequence.
ACCESSION   DATRMA010000092 DATRMA010000000
VERSION     DATRMA010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5693
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.093"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            113..1258
                     /locus_tag="VBC28_001784"
     CDS             113..1258
                     /locus_tag="VBC28_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529591.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HEO6815744.1"
                     /translation="MGQEPIIEYQNINKVYGENVAVEDINLKIYPGDFVCFIGTSGSG
                     KTTLMRMVNHMLKPTNGTLLFKGKDISTINPIELRRRIGYVIQNIGLMPHMTIYENIV
                     LVPKLLKWSEEAKRAKARELIKLVELPEEYLDRYPSELSGGQQQRIGVIRALAADQDI
                     ILMDEPFGALDPITREGIQDLVKSLQEEMGKTIILVTHDMDEALKLATKIIVMDNGKM
                     VQEGTPNDLLHHPATSFVEQMIGEERLLHAQADITPVKQIMLNNPVSITAEKTLTEAI
                     TLMRQKRVDSLLVTDNGKLIGFIDLESLSSKYKKDRLVSDILKHTDFYVMEDDLLRNT
                     AERILKLGLKYAPVVDHENNLKGIVTRASLVDMLYDIIWGDTETEDQ"
     gene            1258..1893
                     /locus_tag="VBC28_001785"
     CDS             1258..1893
                     /locus_tag="VBC28_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000247049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6815745.1"
                     /translation="MVNFLSQYGMQILVKTWEQVYISFFAIALGIAIAVPLGVVLTRF
                     PKVAKIIIAIASMLQTIPSLALLALMIPLFGIGKIPAIVALFIYSLLPILRNTYIGMN
                     NVNPTLKDCAKGMGMKPIQSIFQVELPLATPIIMAGIRLSTIYVIAWATLASYIGAGG
                     LGDLIFSGLNLFQSKLILGGTIPVIILSLIIDYLLGLLETALTPRTTRREA"
     gene            1896..2822
                     /locus_tag="VBC28_001786"
     CDS             1896..2822
                     /locus_tag="VBC28_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HEO6815746.1"
                     /translation="MLKKSHFLQIFTLCLALLTISGCQLTDTKKSGHTTIKVAAQSST
                     ESSIMANIITELIHHELGYNTTLISNLGSSTVTHQALLRGDADIAATRYTGTDITGTL
                     GLKAVKDPKEASKIVKTEFQKRYNQTWYPTYGFSDTYAFMVTKEFARQNKITKISDLK
                     KLSTTMKAGVDSSWMNREGDGYTDFAKTYGFEFSHIYPMQIGLVYDAVESNKMQSVLG
                     YSTDGRISSYDLEILRDDKKFFPPYEASMVVNNSIIKKDPKLKKLLHRLDGKINLKTM
                     QNLNYMVDDKLLEPSVVAKQFLEKNHYFRGDK"
     gene            2823..3473
                     /locus_tag="VBC28_001787"
     CDS             2823..3473
                     /locus_tag="VBC28_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6815747.1"
                     /translation="MKQMNTFQQFIYYFQHNGSYILEQFIHHFLISVYGVILAAIVGI
                     PLGIFIARRGKIKTFVMGIANVIQTIPSLAMISIIMLGLGLGIKTVVATVFLYSLLPI
                     ITNTYTGIRNVDSDLLDAAKGMGMTKRQRLFMVELPLSISVIMAGLRNALVVAIGITA
                     IGAFVGGGGLGDIIIRGTNATNGGAIILAGSLPTALMAIFSDLILGGIQRMLEPRK"
     gene            complement(3535..4716)
                     /locus_tag="VBC28_001788"
     CDS             complement(3535..4716)
                     /locus_tag="VBC28_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO6815748.1"
                     /translation="MNINGIKLLSSRAVSKLGDVFYDYGNSTWIASMGGLGQKILGIY
                     QIVELLVSIVLNPFGGALADRFQRRKILLITDAICAIMCFLLSFIGDDKVMVYGLIVA
                     NAILAVSNAFSSPAYKSYIPEIVDKADIITYNANLETIVQIISVSSPVLGFLIFNNFG
                     IRITLIVDAITFLISFLFLYAIKVERVQLSKQEKVAIKNILADIADGFTYIKKEKEIM
                     FFLIIAALLNTFLAMFNYLLPFTNSLLKTSGAYATILSISAIGSIIGALIARKIKSSI
                     NSMLSMLVFSSLGVIVMGFPSLFELPIWIPYSGSFLFNSLLTMFNIHFFSQVQIRVDE
                     AYMGRVMSTIFTIAIMFMPIGTLFMTIFSFALSNVSFIVIGCAIAILGGLGFSYSKKQ
                     F"
     gene            complement(4789..5649)
                     /locus_tag="VBC28_001789"
     CDS             complement(4789..5649)
                     /locus_tag="VBC28_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MutR family transcriptional regulator"
                     /protein_id="HEO6815749.1"
                     /translation="MFKYVPIFREFRLNRQFSLKQVASNELSVSQLSRFERGESDLSL
                     TKFLGALEAIDLSISEFMDRVNKYQKSDQISLMSQMAQYHYQRDVAGLEKMISVEEGK
                     LKKDSSDIRCRLNIVLFRGMICECDSSRKMSEEDLCFLSDYLFQKDSWEISDYILIGN
                     LYRYYNTRHICQLVKEVINQKEYYRDIYTNRNVVEATLLNVVETLIERRALEEATFFL
                     EKVEALLNNERNAYHRIILLYEKGFLAYAKGDSRGIQSMKQAIFCFQAIGSKHHVENF
                     QEHFNRVTRL"
BASE COUNT         1925 a         1013 c          928 g         1827 t
ORIGIN      
        1 agactcaagc ttaacgattc tttttaaaat cattaaattt taaaacaaat ttcagacatg
       61 ttgccaaagt tttgatatta ttactataat atagtttgta gaggagaata atatgggcca
      121 agaacctatc atcgaatatc aaaatatcaa taaagtgtat ggggaaaatg ttgcggttga
      181 agacattaac cttaaaattt accctggtga ttttgtttgt ttcatcggta cgagtggatc
      241 aggtaaaaca acattaatgc gtatggttaa ccatatgtta aaaccaacaa atggtactct
      301 attatttaag ggaaaagata tttctactat taaccccatt gaattaagac gcagaattgg
      361 atatgttatc caaaacattg gtttaatgcc tcatatgacc atttacgaaa atatagttct
      421 tgtaccaaaa ttattgaaat ggtcagaaga agctaaaaga gctaaagcaa gggaacttat
      481 taaattagtt gaattacccg aagaatattt ggatcgctac cctagtgagt tgtctggcgg
      541 tcagcaacaa cgtatcggtg tcattcgcgc tcttgcagca gaccaagata ttattttaat
      601 ggatgagcct tttggagctc tggatcctat tactagagaa ggtattcaag acttagtcaa
      661 gtctcttcag gaagaaatgg ggaaaactat catcttagtt actcatgata tggatgaagc
      721 cctcaagtta gcaacaaaaa ttattgttat ggacaatggt aaaatggtcc aagaagggac
      781 acccaatgat ctcttacatc atcctgctac tagtttcgtt gaacaaatga ttggagaaga
      841 gcgtctcctt catgcgcagg ctgatattac tcctgttaaa cagataatgt taaataatcc
      901 tgtttcaata actgctgaaa aaacactaac tgaagctatt acactaatgc gccaaaaacg
      961 cgttgactca cttctagtaa ccgataacgg taaattaatt ggttttattg acttagaatc
     1021 tctaagcagt aaatataaga aagatcgact tgtttctgat atcttaaaac atactgattt
     1081 ttatgttatg gaagacgact tacttagaaa tactgctgag cgtattttaa aacttggttt
     1141 aaaatacgct ccagttgttg accatgagaa taacttaaag ggcattgtta ctcgtgcatc
     1201 cctagttgat atgttatacg atattatttg gggcgatact gaaacggagg atcaataatg
     1261 gtcaatttct tatcacagta tggcatgcaa atattagtaa aaacatggga acaagtctat
     1321 atctcattct ttgctatagc acttgggatt gcaattgctg ttccattggg tgtggtctta
     1381 acacgttttc ccaaagtagc caaaattatc attgctatag ctagcatgtt acaaactata
     1441 ccaagtttag ccttattagc tttaatgatt ccattgtttg gaattggaaa aatcccagct
     1501 atcgttgctt tatttattta ttccttgctt cctatactaa ggaatactta tataggtatg
     1561 aataatgtca atccaacact taaagattgt gctaaaggga tgggaatgaa acctattcaa
     1621 tctatatttc aagttgagtt acctttagct actccaatta ttatggctgg tattcgcttg
     1681 tctactattt atgttattgc ttgggctaca ttggcttctt atataggtgc aggtggactt
     1741 ggtgatctca tttttagcgg gttaaatctt tttcagtcca aattaattct agggggcaca
     1801 attcctgtta ttatcctgtc acttattata gattatcttt taggattact ggaaacagct
     1861 ctaacgccca gaacaacaag aagggaggct tagatatgct taaaaaatcg cactttttac
     1921 agatatttac actttgctta gccctcttaa cgatttctgg ttgtcaatta accgatacta
     1981 aaaagtctgg tcataccaca attaaggttg ctgcccaaag ttctacagag tctagtatta
     2041 tggcaaatat tatcaccgaa ttaattcatc acgaattagg atacaacaca actttaataa
     2101 gcaatcttgg ttcctctacg gttactcacc aagctttgct ccgtggtgat gctgacattg
     2161 ctgccacacg ttatacagga acagacatca cagggactct tggtttaaaa gctgttaaag
     2221 accctaaaga agcttctaag attgtaaaaa ctgaattcca aaaacgctac aatcaaactt
     2281 ggtatcctac ttatggtttt tctgatactt atgcattcat ggttactaaa gagtttgcca
     2341 gacagaataa aatcactaag atctctgatc ttaaaaaatt atcaacaact atgaaggcag
     2401 gggttgatag ttcatggatg aatcgcgagg gagatggata cactgatttc gctaaaacat
     2461 acggttttga attttcacat atttacccta tgcaaattgg cttagtctat gatgcggttg
     2521 aaagtaacaa aatgcaatct gtattaggct actccactga cggtcgtatt tcgagctatg
     2581 atttagaaat tttaagggat gataaaaaat tctttcctcc ttatgaagcc tctatggttg
     2641 tcaacaattc tatcatcaaa aaagatccta aactaaaaaa attactccat cgactcgatg
     2701 gtaaaatcaa tttaaaaacg atgcaaaacc ttaattatat ggtagatgat aaacttttag
     2761 aaccttcagt tgttgccaaa caatttttag aaaaaaacca ttattttaga ggagataaat
     2821 aaatgaaaca aatgaatact ttccaacaat ttatctacta cttccaacat aatggtagtt
     2881 atatcttaga acaatttatc caccactttt taatttcagt ttacggcgtt attttagctg
     2941 caattgttgg tatccctcta ggtatcttca tcgctagacg tggtaaaatt aaaacctttg
     3001 ttatgggaat cgcaaatgtc atccaaacca ttccttcttt agctatgatc tctatcatta
     3061 tgttaggtct agggctaggt attaaaacag tagttgcaac tgtctttctt tattctcttc
     3121 tgccaatcat cacaaatact tacactggta ttagaaatgt tgactccgac ttactagatg
     3181 ctgctaaagg aatgggcatg actaagcgtc aacgtttatt tatggttgag ttaccactat
     3241 ctatttccgt tatcatggct ggacttagaa atgcccttgt tgtagcaatt ggtattacag
     3301 ccattggtgc tttcgtcggg ggaggcggac ttggagatat catcataaga ggaactaatg
     3361 caacaaatgg tggtgctatt atcttagcag gatctcttcc aacagccctt atggcaattt
     3421 tctctgattt aatcctaggc ggtatccaac gcatgcttga accaagaaaa taactattaa
     3481 aaacttgtca gtagagacaa gtttttttga tgtttcagaa taatttctta agttctaaaa
     3541 ttgtttttta ctatagctaa atcctaatcc tcccaatata gcaatggcac atcctataac
     3601 aataaagcta acatttgata aggcaaatga aaagatagtc ataaatagtg ttcctatagg
     3661 cataaacata atagcaatag taaatatagt cgacatcaca cgccccatat acgcttcatc
     3721 gaccctgatt tgtacctgac tgaaaaaatg aatattaaac attgtcagaa gactattaaa
     3781 aagaaaacta ccactgtaag gtatccatat cggtaattca aatagagatg ggaatcccat
     3841 aactatgact cccaaactac tgaatactag catagatagc atagaattaa tactagattt
     3901 aatttttcta gcaataagcg ctccaataat tgatccgata gctgatatac taagtattgt
     3961 tgcataagca cctgaagttt ttaataggga gttagtaaaa ggtaaaagat aattgaacat
     4021 tgctaggaaa gtgtttaata aagcagcaat aattagaaaa aacataattt ctttttcttt
     4081 tttgatgtaa gtaaatccat ctgcaatatc agctaaaata ttttttatcg ctaccttttc
     4141 ttgttttgaa agctgtaccc tttccacttt aatagcataa agaaacagga aagagatcaa
     4201 aaaagtaatc gcatcaacaa ttaaagtaat cctaatacca aaattattaa aaataaggaa
     4261 acctaaaaca ggggaactta cactaataat ttgcacaatg gtttccagat tagcattata
     4321 ggtaatgata tcagctttat ctactatttc aggaatataa gatttataag ccggagaaga
     4381 aaaagcattg gaaaccgcta agatagcatt agcaactatt agaccataca ccattacttt
     4441 atcatctcca ataaaagaaa gtaagaaaca catgatagca cagatagcat ctgttattag
     4501 taaaattttt cttcgttgaa agcgatcagc taaggcgcct ccaaaagggt tgaggacaat
     4561 agaaaccaac aactctacta tctgataaat acctaaaatc ttttgtccta gaccacccat
     4621 cgaagcaatc caagtactat ttccataatc ataaaacaca tcaccaagtt tactgactgc
     4681 tcgactagat agtaatttta taccattaat attcataatt ccctccttag ttcgacatta
     4741 tagcaaacca aaaggagaaa tattaaaaaa tcccataaat gggatttttt ataatcttgt
     4801 taccctatta aagtgttcct gaaaattttc aacatgatgt ttagaaccaa ttgcttggaa
     4861 gcaaaaaata gcttgcttca tgctttgtat tcctcgacta tcccctttag cataggcaag
     4921 aaatcctttt tcatagagta aaataatacg gtgataagca ttccgctcat tatttaaaag
     4981 tgcttcaact ttctctagaa aaaacgttgc ttcttctaga gcgcgccgtt ctattagtgt
     5041 ctctaccaca tttaggaggg ttgcttcgac aacattgcgg tttgtataaa tatctcgata
     5101 atattccttt tgattgataa cctctttaac aagttggcaa atatgccttg tgttgtaata
     5161 ccggtaaaga tttccaatca aaatatagtc actaatttcc caactatcct tttgaaagag
     5221 gtaatccgac aaaaagcaaa ggtcttcttc cgacattttt cttgaggagt cacattcaca
     5281 tatcattcct ctaaaaagaa ctatgtttaa acgacaacgt atatctgaac tatccttttt
     5341 tagtttacct tcttccacag aaatcatctt ttctaaccca gctacgtctc tttgataatg
     5401 atattgagcc atctgagaca ttaaagatat ctgatcagat ttttgatatt tatttactct
     5461 atccataaat tcgctaattg ataaatcaat tgcctctaaa gcccctaaaa attttgttaa
     5521 tgataaatct gattcacccc tctcaaatcg tgataactga gaaacagaaa gttcatttga
     5581 agcaacctgt ttaagagaaa actgtcgatt aagtcgaaat tccctaaaaa tagggacata
     5641 tttaaacatt agtattcctt tctaaccgta atctcattgg taaacttatg agg
//
LOCUS       DATRMA010000093         5674 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.094, whole genome shotgun sequence.
ACCESSION   DATRMA010000093 DATRMA010000000
VERSION     DATRMA010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5674
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.094"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            118..747
                     /gene="udk"
                     /locus_tag="VBC28_001790"
     CDS             118..747
                     /gene="udk"
                     /locus_tag="VBC28_001790"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HEO6815750.1"
                     /translation="MRKKPIIIGVTGGSGGGKTSVSRAILSNFPDQKITMIEHDSYYK
                     DQSHLTFEERVKTNYDHPLAFDTNLMIEQLNELIEGRPVDIPVYDYTKHTRSDRTIRQ
                     EPQDVIIVEGILVLEDQRLRDLMDIKLFVDTDDDIRIIRRIKRDMEERDRSLDSIIEQ
                     YTEVVKPMYHQFIEPTKRYADIVIPEGVSNIVAIDLINTKVASILNEAK"
     gene            834..1331
                     /locus_tag="VBC28_001791"
     CDS             834..1331
                     /locus_tag="VBC28_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEO6815751.1"
                     /translation="MNKSKKIENYQLLLLQAQALFSDETNALANLSNASAMLNAMLPN
                     SVFTGFYLFDGEELILGPFQGGVSCVHITLGKGVCGESAQTAKTLIVDDVTKHANYIS
                     CDSKAMSEIVVPMFKNGKLLGVLDLDSSLVADYDEIDQEYLEKFVGILVEHTIWNLDM
                     FGVEK"
     gene            1331..2995
                     /gene="dnaX"
                     /locus_tag="VBC28_001792"
     CDS             1331..2995
                     /gene="dnaX"
                     /locus_tag="VBC28_001792"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEO6815752.1"
                     /translation="MYQALYRKYRSQTFDEMVGQSVISTTLKQAVSSKKISHAYLFSG
                     PRGTGKTSAAKIFAKAMNCPNQINGEPCNHCDICRDITNGSLEDVIEIDAASNNGVDE
                     IRDIRDKSTYAPSRATYKVYIIDEVHMLSTGAFNALLKTLEEPTENVVFILATTELHK
                     IPATILSRVQRFEFKAIKLLAIRDHLAQILDKEAISYDLDALTLVARRAEGGMRDALS
                     ILDQALSLAKDNHISLDVAEEITGSISLSAIDDYVSNILAHDTTEALAKLEVIFDSGK
                     SMSRFATDLLMYLRDLLVVQAGGEDSHSSDTFIANLNVKQDILFEMIDKVTSVLPEIK
                     NGSHPKVYAEMMTIQLSEMVEKNSSNIPADVTAELDSLRRELKSLKNEMSQLSRADQS
                     SSTQKVKVNNKTFTFKVDRTKILTIMEETVVDSQRSREYLEALKSAWNEILDNITAQD
                     RALLMGSEPVLANSENAILAFDAAFNAEQAMKRTDLNDIFGNIMSKAAGFSPNILAVP
                     RNDFNQIRSDFAKKMKAQKTETEPEVNHQIPEDFSYLAERIAIVED"
     gene            3084..3278
                     /locus_tag="VBC28_001793"
     CDS             3084..3278
                     /locus_tag="VBC28_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3272 domain-containing protein"
                     /protein_id="HEO6815753.1"
                     /translation="MTKRQFIFMALLCSFETYFFNQSVMDGSWIFAIFWGVLLLRDLQ
                     KVYAISKFTKELIKSTKKKD"
     gene            complement(3259..4194)
                     /locus_tag="VBC28_001794"
     CDS             complement(3259..4194)
                     /locus_tag="VBC28_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     synthetase/biotin operon repressor"
                     /protein_id="HEO6815754.1"
                     /translation="MKTYEKIYQILSKNNNFISGETMANQLNISRTAIWKGIKTLEEL
                     GLEIESVTNKGYRLVSGDILLPEQLEQEIGIKVSLNNNSASTQLDAKMGIESKLKTPH
                     LFLAPNQKKAKGRFDRPFFTSNQGGIYMSLLLQPNVPIEDIKPYTVMVASSAVKAISR
                     LTGITPEIKWVNDIYLDNKKIAGILTEAIASVESGLVTNVIIGLGINFYIKEFPRALT
                     KRAGSLFTEQPTITRNQLITEIWNLFFNIPLEDHLKVYREKSLVLDRTVSFMDGQTMY
                     SGKAIDITDKGYLVVELDDGQLKTLRSGEISLSSW"
     gene            4379..5575
                     /locus_tag="VBC28_001795"
     CDS             4379..5575
                     /locus_tag="VBC28_001795"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEO6815755.1"
                     /translation="MSERKLFTSESVSEGHPDKIADQISDAILDAILEQDPDAHVAAE
                     TAVYTGSVHVFGEISTTAYVDINRVVRNTIAEIGYDKAEYGFSAESVGVHPSLVEQSP
                     DIAQGVNEALEVRGSLEQDPLDLIGAGDQGLMFGFAVDETPELMPLPISLAHQLVKKL
                     TDLRKSGELTYLRPDAKSQVTVEYDENDQPIRVDAVVISTQHDPNVTNDQLHKDVIEK
                     VINEVIPSHYLDDQTKFFINPTGRFVIGGPQGDSGLTGRKIIVDTYGGYSRHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAADLAKKVEVQLAYAIGVAQPVSVRVDTFGTGVIA
                     EADLEAAVRQIFDLRPAGIINMLDLKRPIYRQTAAYGHMGRTDIDLPWERVDKVQALK
                     DFIASK"
BASE COUNT         1840 a          898 c         1087 g         1849 t
ORIGIN      
        1 atcattgtta ttataacatg ctttttattt ttagagcgtt ttcaataaaa tttagacttt
       61 aaaaaaaata agtttatagt acaatagata gaggataaat ttaatttgga gtgacaaatg
      121 cgtaaaaaac ctattattat tggtgttaca ggtggatctg gtggtggtaa aaccagtgtt
      181 tctcgggcga ttttatcaaa tttcccagat caaaaaatta ccatgattga acatgattct
      241 tactataaag atcaaagtca tttaactttt gaagaacgtg taaaaaccaa ttatgatcat
      301 ccccttgctt ttgataccaa tttgatgatt gagcaattga acgaattgat tgagggacgt
      361 ccggtcgata ttccggttta tgattacaca aaacataccc gtagtgatag aactatccgc
      421 caagaacccc aggatgtcat tattgttgag ggaattttgg tattagaaga tcagcgtttg
      481 cgtgatttaa tggatatcaa attatttgtt gatacagatg atgatattcg tattattcgt
      541 cgtattaagc gtgatatgga agagcgcgat agaagtttag atagtattat tgaacagtat
      601 acagaagttg taaaacctat gtatcatcaa tttatcgaac caacaaagcg ttatgcggat
      661 attgtaattc cagaaggagt aagtaatatt gtcgctattg atttaatcaa taccaaagtg
      721 gctagcatct taaatgaagc aaaataaaca caatggtttg tgtttatttt tttgttttat
      781 ataggatggt tatttagttt tagtaacttt caaattatgc tataataagt gctatgaaca
      841 agtctaagaa aatcgaaaat tatcaattat tattactaca agcgcaagct ctattctcag
      901 atgaaacaaa tgctcttgcc aacttatcaa atgcttcagc tatgctaaat gctatgcttc
      961 caaattctgt atttacaggc ttttatttat ttgatggaga agagttaatt cttggccctt
     1021 tccagggtgg tgtatcatgt gtgcatatta ctttaggaaa aggtgtttgt ggtgaatctg
     1081 cacaaactgc taagacgctg atcgttgatg atgttacaaa gcatgctaac tatatctcct
     1141 gtgattcaaa agctatgagt gaaatcgtag tacctatgtt taaaaatggc aaacttctag
     1201 gagttctaga tttagattct tctttagtag cagattatga tgagattgat caagaatact
     1261 tagaaaaatt tgtaggtatt ctagtagaac atacgatttg gaatttggat atgtttggag
     1321 ttgaaaagta atgtatcagg ccctttatcg gaaatatcgt agccaaacat ttgatgaaat
     1381 ggttggacaa agtgttattt caactacttt aaagcaagct gtgtccagca aaaaaattag
     1441 ccatgcttac cttttttcag gacctagggg aactggaaaa acaagtgctg ctaaaatatt
     1501 tgcaaaagcg atgaattgcc caaatcaaat taatggcgaa ccttgtaatc attgtgatat
     1561 ttgtcgagat attacaaatg gtagtttaga ggatgtcatt gaaattgatg ctgcgtcaaa
     1621 taatggtgtc gatgaaattc gtgatatcag ggacaaatca acttatgctc caagtagggc
     1681 aacctataaa gtttatatta ttgatgaggt tcatatgttg tctacaggtg ccttcaatgc
     1741 actattgaaa acacttgaag agccaacaga aaatgttgtt tttatattag caacaacaga
     1801 actccataaa atccctgcaa caatcttatc tcgtgtccag cgctttgaat ttaaagcgat
     1861 taaattatta gctattcgag atcatttagc acagatttta gataaagaag ctatttcata
     1921 tgatttggat gctctgacac tagtcgctag acgagccgaa ggtggtatgc gtgatgcttt
     1981 atcaattctc gatcaagcct tgagtttagc gaaagataac cacatttctt tagatgtagc
     2041 agaagaaatt acagggagca tttccttatc tgctatagat gattatgtat cgaatatatt
     2101 ggctcacgat acaactgaag cactcgctaa acttgaagta atctttgata gtggcaaaag
     2161 tatgagtcgt tttgcaacag atttattgat gtacttaaga gatttacttg ttgttcaagc
     2221 aggtggtgaa gatagtcatt caagtgatac ttttatagcc aatcttaatg ttaaacaaga
     2281 tattttattt gaaatgattg ataaagtgac atctgttctt cctgagataa aaaatggtag
     2341 ccatcctaaa gtttatgcag agatgatgac catccaattg tctgaaatgg tagaaaaaaa
     2401 ttcgtcaaac atacccgcag atgtgacagc agaattagat agtcttcgtc gagagttaaa
     2461 atcgttaaaa aacgaaatga gccaactctc tcgagcagat caaagttcat caactcaaaa
     2521 agtaaaggtt aataataaga cattcacatt taaagttgac cgtacaaaga ttttgaccat
     2581 tatggaagaa acggttgtag atagtcaacg ttcacgtgaa tacttagaag ctttaaagag
     2641 cgcttggaac gagatattag ataatattac ggctcaagat agagccttat taatgggatc
     2701 tgaacctgtc ttagctaata gcgagaatgc tattttggca tttgatgcgg cctttaatgc
     2761 tgaacaagct atgaaacgta ccgatctcaa tgatatattt ggtaatatta tgagtaaagc
     2821 agcaggtttt tcacctaata ttttagctgt acctagaaat gattttaatc aaattcggag
     2881 cgattttgca aagaaaatga aagctcaaaa gacggaaaca gaacctgaag ttaatcatca
     2941 aattccagaa gacttttctt atctagcaga acgaatagca atcgttgaag attaaaacgt
     3001 aactaaaaat agttgcctgt aacaggcaac tatttttttg ataagttttt taattattta
     3061 gtagattctg ttataatggt gatatgacaa aacgacaatt tatttttatg gcgcttttat
     3121 gctcttttga aacttatttt tttaatcaat ccgttatgga tggctcctgg atttttgcta
     3181 ttttttgggg agttttatta cttcgagatc ttcaaaaggt gtatgcgatt tcaaaattca
     3241 caaaagagct tatcaaatct accaagaaga aagactgatt tcgccactgc gaagtgtttt
     3301 caactgacca tcatctaact caaccactaa atatccttta tctgtaatat caatagcttt
     3361 cccagaatac attgtttgac catccataaa gctaacggtt ctatctaaga ctagggattt
     3421 ttctctatat acctttaagt gatcctctag tggaatattg aaaaagagat tccagatttc
     3481 tgtaatcaat tgatttctag ttatggtcgg ttgctctgta aagagactac ctgcgcgctt
     3541 agttaacgcc ctagggaact ctttgatata gaaattgata cctaatccta taataacatt
     3601 cgttaccaac ccactctcaa cagaagcgat agcctcggtt aaaataccag ctattttttt
     3661 attatctaaa taaatatcat tgacccactt aatttcagga gtaattcctg ttaaccgtga
     3721 gatagctttt acagcactag aggctaccat gacagtataa ggttttatat cttcaatcgg
     3781 tacattaggt tgtaataaaa gagacatata gatgccgcct tgatttgatg taaagaaagg
     3841 tctatcaaaa cgtcctttag cttttttttg attaggagct aaaaaaaggt gtggagtctt
     3901 taatttactt tctatcccca ttttagcatc tagttgggtt gaagcactat tattattaag
     3961 actaacctta ataccaattt cttgttcgag ttgttctggt aataatatgt cacctgatac
     4021 caatcggtag cccttgttag tgacagactc aatctctagg cctaattctt ctaacgtttt
     4081 tatacctttc caaatagctg tgcgggaaat attaagttga tttgccatag tttctccgct
     4141 aataaagttg ttattttttg atagtatttg ataaattttt tcgtaggttt tcataaatct
     4201 agtatagtat aaatcgagaa aaaagactaa agaaaataga gatatttttt tatttattgg
     4261 taacttattt cacaaaagat gaaatagtgg tacaatagct atatgagtcc cgaaaaggta
     4321 gcctagaggc tagttctttg taactcgaca cattttacat tttagggggg acatttttat
     4381 gtcagaacgt aaacttttca cgtctgagtc agtatctgag ggacatcctg ataagattgc
     4441 tgaccaaatt tccgatgcga ttttagatgc aattttagag caagatccag atgcccacgt
     4501 agcagccgaa acagctgttt atacagggtc ggttcatgta tttggcgaaa tatcaacgac
     4561 agcctatgtt gatattaacc gagttgtccg taacacaatc gctgaaattg gctatgataa
     4621 agctgaatat ggcttctcag ctgaatctgt tggagtgcat ccgtcactcg ttgaacagtc
     4681 gccagacatt gcacagggag tcaatgaagc tcttgaggta cgtggttctc ttgaacaaga
     4741 tcctttagat ttaattggtg caggtgacca aggtttgatg tttgggtttg ctgttgatga
     4801 aacacctgag ttaatgccat tgccaatttc tttagctcat cagttagtta agaaattgac
     4861 agatctacgt aagtctgggg aactcactta tcttcgacca gatgcaaaat ctcaagtaac
     4921 ggttgaatat gatgaaaatg atcaaccaat tcgtgtggat gcagtagtta tttcgacaca
     4981 gcatgatcct aatgtaacta atgaccaact ccataaggac gtgattgaga aagttatcaa
     5041 tgaagttatt ccaagccatt atttagatga ccaaacaaaa ttctttatca atcctacagg
     5101 gcgctttgtt attggaggcc ctcaaggaga ttcaggacta acaggtcgta agattattgt
     5161 agatacttat ggtggttatt ctcgtcatgg tggtggtgca ttttcaggta aagatgctac
     5221 taaagtagat cgttcagcat cttatgcagc tcgttatatt gcaaaaaata ttgttgcagc
     5281 tgatttagct aaaaaagtag aagtacaact tgcttatgca attggtgttg cccagcctgt
     5341 ctctgtgcgt gtagatacat ttggaacagg tgttatagca gaagcagacc tagaggcagc
     5401 tgtacgtcaa atatttgatt tgcgaccagc aggcattatt aatatgttgg acctcaaacg
     5461 acctatttat cgacaaacag cagcctatgg gcatatggga cgtactgata tcgatttgcc
     5521 atgggagcga gttgataaag tccaggcctt aaaggatttt attgctagca aataataaaa
     5581 agcgtaaacg aaagtttacg ctttttttgc tcatgataat ttatagaaca tttataaaat
     5641 cttataataa actggttaag tataggaaat actg
//
LOCUS       DATRMA010000094         5670 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.095, whole genome shotgun sequence.
ACCESSION   DATRMA010000094 DATRMA010000000
VERSION     DATRMA010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5670
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.095"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            60..929
                     /gene="cas1"
                     /locus_tag="VBC28_001796"
     CDS             60..929
                     /gene="cas1"
                     /locus_tag="VBC28_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000929484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR-associated endonuclease Cas1"
                     /protein_id="HEO6815756.1"
                     /translation="MAGWRTVVVNTHSKLSYKNNHLIFKDSYQTEMIHLSEIDILIME
                     TTDIVLSTMLIKRLVDENILVIFCDDKRLPTAMLMPYYARHDSSLQLSRQMSWIEDVK
                     ADVWTSIIAQKILNQSFYLGECSFFEKSQSIMNLYHDLEPFDPSNREGHAARIYFNTL
                     FGNDFSREQDNPINAGLDYGYSLLLSMFAREVVKCGCMTQFGLKHANQFNQFNLASDI
                     MEPFRPIVDRIIYENRQSDFVKMKRELFSMFSETYSYNGKEMYLSNIVSDYTKKVIKS
                     LNSDGNGIPEFRI"
     gene            926..1267
                     /gene="cas2"
                     /locus_tag="VBC28_001797"
     CDS             926..1267
                     /gene="cas2"
                     /locus_tag="VBC28_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HEO6815757.1"
                     /translation="MSYRYMRMILMFDMPTETAEERKAYRKFRKFLLSEGFIMHQFSV
                     YSKLLLNNTANNAMIGRLKVNNPKKGNITLLTVTEKQFARMVYLHGERNTSVANSDSR
                     LVFLGDSYDQD"
     gene            1254..1919
                     /gene="csn2"
                     /locus_tag="VBC28_001798"
     CDS             1254..1919
                     /gene="csn2"
                     /locus_tag="VBC28_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II-A CRISPR-associated protein Csn2"
                     /protein_id="HEO6815758.1"
                     /translation="MIKINFPILDEPLVLSNATILTIEDVSVYSSLVKHFYQYDVDEH
                     LKLFDDKQKSLKATELMLVTDILGYDVNSAPILKLIHGDLENQFNEKPEVKSMVEKLA
                     ATITELIAFECLENELDLEYDEITILELIKALGVKIETQSDTIFEKCFEIIQVYHYLT
                     KKNLLVFVNSGAYLTKDEVIKLCEYINLMQKSVLFLEPRRLYDLPQYVIDKDYFLIGE
                     NMV"
     repeat_region   2020..2649
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=2020..2055
                     /rpt_unit_seq="gttttagagctgtgctgtttcgaatggttccaaaac"
     gene            3207..3623
                     /gene="ndk"
                     /locus_tag="VBC28_001799"
     CDS             3207..3623
                     /gene="ndk"
                     /locus_tag="VBC28_001799"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEO6815759.1"
                     /translation="MEQTFFMIKPDGVKRGFIGEVISRIERRGFSIDRLEVRYADADI
                     LKRHYAELTDRPFFPTLVDYMTSGPVIIGVISGEEVISTWRTMMGSTNPKDALPGTIR
                     GDFAQAPSPNQATCNIVHGSDSPESATREIAIWFNN"
     gene            3759..5591
                     /gene="lepA"
                     /locus_tag="VBC28_001800"
     CDS             3759..5591
                     /gene="lepA"
                     /locus_tag="VBC28_001800"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HEO6815760.1"
                     /translation="MNIEDLKKRQEKIRNFSIIAHIDHGKSTLADRILEKTETVSSRE
                     MQAQLLDSMDLERERGITIKLNAIELNYTAKDGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAILVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRAEVEDVI
                     GLDASEAVLASAKAGIGIEEILEQIVEKVPAPTGEVDAPLQALIFDSVYDAYRGVILQ
                     VRIVNGMVKPGDKIQMMSNGKTFDVTEVGIFTPKAVGRDFLATGDVGYIAASIKTVAD
                     TRVGDTITLANNPAIEPLHGYKQMNPMVFAGLYPIESNKYNDLREALEKLQLNDASLQ
                     FEPETSQALGFGFRCGFLGLLHMDVIQERLEREFNIDLIMTAPSVVYHVNTTDGEMLE
                     VSNPSEFPDPTRVDSIEEPYVKAQIMVPQEFVGAVMELAQRKRGDFVTMDYIDDNRVN
                     VIYQIPLAEIVFDFFDKLKSSTRGYASFDYEISEYRRSQLVKMDILLNGDKVDALSFI
                     VHKEFAYERGKLIVDKLKKIIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKAGKKRMKAIGSVEVPQEAFLSVLSMDDDDKK"
BASE COUNT         1796 a          872 c         1152 g         1850 t
ORIGIN      
        1 atctattact ggactttatg aaacacgtat tgatttgggt aagttaggag aagattgata
       61 tggcaggttg gcgaaccgtt gttgtaaata cacattctaa gctctcttat aaaaataatc
      121 atctgatttt taaagattct tatcagacgg aaatgattca tctatcagag attgacattc
      181 taatcatgga aacaacagat atcgttttgt cgaccatgct gattaaacgt ttggttgatg
      241 aaaatatttt agttatattt tgtgacgata aacgcttgcc aacagctatg ttaatgccgt
      301 actatgccag acatgattcg agtttacaat tatctaggca gatgtcatgg attgaagatg
      361 tcaaagcaga tgtttggaca tcaattattg cacaaaaaat tttgaatcag tctttttatc
      421 tcggtgagtg ttctttcttt gaaaaatccc agtctattat gaatctctac catgacttag
      481 aaccttttga tccttctaat cgtgaggggc atgctgctag gatttatttc aatacacttt
      541 ttggaaatga tttttcaaga gagcaggata atccaataaa tgctggttta gactacggat
      601 attcattgct tttgagtatg tttgcgcgtg aagttgttaa gtgtggttgc atgacacaat
      661 ttggcttgaa gcatgctaat caatttaatc agttcaacct agcaagcgat attatggaac
      721 catttcgccc aatcgttgat aggattattt atgaaaatag gcagagtgat tttgtcaaaa
      781 tgaaaagaga actcttttct atgttttcag agacatacag ctacaatggt aaagaaatgt
      841 atctctcaaa tattgtcagc gactatacca aaaaagttat taagtcgcta aatagtgatg
      901 ggaatggaat tccggagttt aggatatgag ttatcggtat atgcgaatga ttttaatgtt
      961 tgatatgcct actgaaacag cagaagaacg gaaggcgtat cgtaagttta gaaagtttct
     1021 cttgagcgaa ggctttatca tgcatcagtt ttctgtttat agtaaattat tactcaataa
     1081 tacagctaat aatgctatga taggtcggct taaagtgaat aatcctaaaa agggtaatat
     1141 cacactctta acagttacgg aaaaacaatt tgcgagaatg gtttacctcc atggagaacg
     1201 caacacaagt gttgccaact ctgatagtcg cttggttttc ctaggagatt cttatgatca
     1261 agattaattt tccaatttta gatgaaccat tagtgttaag taatgctacg attttaacga
     1321 tagaagatgt ttcagtttat tcttcattgg tgaaacattt ttatcaatat gacgtagatg
     1381 aacatttgaa attatttgat gataagcaga aaagtctgaa ggcaacagag ttaatgctgg
     1441 ttacagatat cttaggatac gatgtcaact cagcacctat tctaaagttg atacatggtg
     1501 acttagaaaa tcaattcaac gaaaagccag aagtgaaatc aatggtagaa aaattagcag
     1561 ctactattac agaacttatc gcatttgagt gtctagagaa tgagcttgat ttagaatacg
     1621 atgaaattac gattttagaa ctcattaagg cactgggagt caaaattgag acacagagcg
     1681 acactatctt tgaaaaatgt tttgaaatta tacaagttta ccattattta acgaaaaaga
     1741 atctcttggt ttttgttaat agcggagctt atcttaccaa agatgaagtt ataaaattat
     1801 gtgaatacat caatttaatg caaaagtcag tactctttct agaacctaga agactctatg
     1861 atttaccgca atatgttatt gataaggatt atttcttgat aggcgaaaat atggtataat
     1921 attagtaaaa gcacagtaat aacaaggaat catcgaaact gaagtcctgc tgagacgaat
     1981 ggcgcgatta cgaaagctca aaagaaaatt ttctacgagg ttttagagct gtgctgtttc
     2041 gaatggttcc aaaactttta tattacgaag gatactttga taattgtttt agagctgtgc
     2101 tgtttcgaat ggttccaaaa ccatctaaag ctcaagaagt taatactatg tgttttagag
     2161 ctgtgctgtt tcgaatggtt ccaaaacttt aacaaaatca taacctaaag gggtattgtt
     2221 ttagagctgt gctgtttcga atggttccaa aactattatc agatacgcta aaaccagtag
     2281 ttcgttttag agctgtgctg tttcgaatgg ttccaaaact tcaaacggag ttgcttttaa
     2341 aaatggggtg ttttagagct gtgctgtttc gaatggttcc aaaacatcca aaaactggtg
     2401 gacacgggat cctttgtttt agagctgtgc tgtttcgaat ggttccaaaa cctacacgca
     2461 agattacatg gcttctcagt tgttttagag ctgtgctgtt tcgaatggtt ccaaaactgg
     2521 ttatacattt actaatccat cagcattgtt ttagagctgt gctgtttcga atggttccaa
     2581 aacaagctaa ttctcatctc accgagatgg atagttttag agctgtgctg ttattatgct
     2641 aggacatcat tgtggtgttc tagttttttg ttatactgaa ataaattttc agagaatgtg
     2701 ggggaaggcg gtaattagat taattcaaga cgtaattcag aacttagttg gccaagctaa
     2761 cgaaatcacc ccaatttatc agtttgattg ggaaacttat atattggcga ctaaaaaata
     2821 tgaacgtcat ttagaggtgt gtctattagt agaaaattcg aattgttttt cggattcaaa
     2881 gagaatgtgt caataaaaga gatatgaaag gctataattc caaccttatg gttaaagggc
     2941 taggttgttc tacgctttac ttaattatta gtttgacagc gttggttctt ttagtgattg
     3001 ctggtgtttt ctttgtcatt aatacttgca agcttacaag gaaagcagtg gagaacctat
     3061 ccataatcaa ctaacagcta tgatggatct cacctattaa tatagttaag gagtgtccaa
     3121 tactcctttt ttgaaataca gttggaagtt acagaaaaca tattttcatg ttataatagt
     3181 ggagaaagaa atgaggtgct aatattatgg aacaaacctt ttttatgata aaacctgatg
     3241 gtgtaaaacg tggttttatt ggagaagtta ttagtcgtat tgagcgtcga ggcttctcta
     3301 ttgatcgctt agaggttaga tatgcagatg cagatatttt gaaaagacat tatgcagaac
     3361 tgacggatag accattcttt ccaacattag tagattatat gactagtggt ccagtaatca
     3421 ttggagttat ttcgggagaa gaggtcattt caacttggcg caccatgatg gggagtacta
     3481 accccaaaga tgcccttcca ggaactattc gaggagattt tgcacaagct ccctctccaa
     3541 accaagcgac atgtaatatt gttcatgggt cagactcacc tgaatctgca actcgcgaga
     3601 tagccatttg gttcaataac taatagactt aatgaagatt gatgttcaag tgttaattct
     3661 cttttacatc ggtcttcttt ttgatataat attctagagt ctataggact gttttaatac
     3721 tttagaagtt aaacatatca aattgtatag gaaatagaat gaatattgaa gatttgaaga
     3781 aacgtcaaga aaagattaga aacttttcga ttatcgctca tattgaccat ggaaaatcaa
     3841 ccttagcaga ccgtatttta gagaaaacag aaacggtttc tagtcgtgaa atgcaggcgc
     3901 aactgttgga ctctatggat ctagaacgtg agcgtggaat tactataaag ttaaatgcta
     3961 ttgagcttaa ttatactgct aaagatggtg aaacttacat ttttcacttg attgacacgc
     4021 cagggcatgt tgacttcaca tatgaagtat cacgttcatt ggcagcttgt gagggtgcta
     4081 tactggttgt tgatgcagcc caaggaattg aagctcaaac tctggcaaat gtttatttgg
     4141 ctctagacaa tgatttagag atactaccag ttattaataa aattgattta ccagcagcag
     4201 atccagaacg tgtacgtgca gaagtagagg atgttattgg tctagatgct tcagaagctg
     4261 tgcttgcctc agctaaggca gggattggta ttgaagaaat tttagagcag atcgttgaaa
     4321 aagttcctgc accgactggg gaagtagatg ctcctcttca agctctaatt tttgattctg
     4381 tttatgatgc ttatcgtgga gtaattcttc aagtaaggat tgtaaatggt atggttaagc
     4441 caggagataa gattcaaatg atgtctaatg gtaaaacctt cgacgttact gaggttggta
     4501 ttttcactcc caaagcagtt ggacgtgatt ttttagcaac tggagatgta gggtatattg
     4561 ctgcctcaat taaaacggtt gctgatacac gtgttggtga taccattacc cttgctaata
     4621 acccagcaat agaacccttg catggctata agcaaatgaa cccaatggtt tttgccggtc
     4681 tttatccaat tgaatcaaat aagtataacg atctgcgtga agcccttgaa aaattacaat
     4741 tgaatgatgc tagtctacag tttgaaccgg aaacatcaca agcattaggg tttggtttcc
     4801 gttgtggttt cttaggactt ttacatatgg acgttatcca agagcgcttg gaacgagagt
     4861 tcaatattga tcttattatg actgcaccat cagtagttta tcatgttaat acaactgacg
     4921 gtgaaatgct tgaagtttcg aatccatctg aatttcctga tcctactcgt gttgacagca
     4981 ttgaagaacc atatgttaag gcgcaaatta tggttcctca agaatttgtc ggtgctgtaa
     5041 tggagttagc acaacgtaaa cgtggtgact ttgtaacaat ggattatatt gatgataatc
     5101 gtgttaatgt tatttaccaa attcctttag ctgaaattgt gtttgacttc tttgataagc
     5161 ttaaatcatc aacacgtggc tatgctagtt ttgattatga aatttccgaa tatcgtcgct
     5221 cacaattagt taaaatggat attcttttaa atggtgataa agtggatgct ttaagcttta
     5281 ttgttcacaa agaatttgcc tatgaacgtg gtaaattgat cgttgataag ttgaaaaaaa
     5341 tcattcctcg tcagcagttt gaagttccta tccaagcagc tattggacaa aaaattgtgg
     5401 cccgttctga tataaaagca ctacgtaaaa atgtcttagc aaaatgttat ggtggtgacg
     5461 tttcacgtaa acgtaagttg cttgaaaaac aaaaagctgg taaaaagcgc atgaaagcaa
     5521 ttggttccgt cgaagttcct caggaagctt tcttaagtgt tttatcaatg gacgatgatg
     5581 acaaaaaata attcagaaag atgatatcat ttatctattt taaagtaact tagcttcgtg
     5641 cttaagttac tttttctttg tctgcaatta
//
LOCUS       DATRMA010000095         5431 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.096, whole genome shotgun sequence.
ACCESSION   DATRMA010000095 DATRMA010000000
VERSION     DATRMA010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5431
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.096"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            complement(<1..429)
                     /locus_tag="VBC28_001801"
     CDS             complement(<1..429)
                     /locus_tag="VBC28_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEO6815761.1"
                     /translation="MRGYRMEERFKKRLQDDISKHFSRQSLILSLLLIALFVLFSLAP
                     QQIGLYKDVNSVSYSYKQLIQKHDTLLDDLGKNSLKPFVSGHLGSADLSKQYYHLRNH
                     LQSQTELLVFSPNQELLFASNSHLGNFFSKSIYISEVLDKA"
     gene            complement(567..1394)
                     /locus_tag="VBC28_001802"
     CDS             complement(567..1394)
                     /locus_tag="VBC28_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEO6815762.1"
                     /translation="MKSQDNLTKEDRKMLRSVFWRSWTMNASRTGATQYHAVGVIYTL
                     LPVINRFYKTDKDKAEALVRHTTWFNATMHINNFIMGLVASMEKKNSEDPDFDASAIT
                     AVKASLMGPISGVGDSFFWGILRVIAAGIGISLASTGSAMGAVVFLLLYNIPAFLIHY
                     YSLYGGYSVGAGFIKKLYESGGIKIVTKTSSMLGLMMVGSMTASNVKFKTILTVAAKG
                     AKEAASIQSYLDQLFVGVVPLLVTILAFWLLRKKVNINWIMFGIMVLGIVLGLLGIC"
     gene            complement(1391..2200)
                     /locus_tag="VBC28_001803"
     CDS             complement(1391..2200)
                     /locus_tag="VBC28_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEO6815763.1"
                     /translation="MIMPATMAALAVLICFGGNYLTGQSMMERPLVVGLVTGMLLGDI
                     KVGILMGASLEALFLGNVNIGGVIAAEPVTATAMATTFTIISNIDQKAAMTLAVPIGM
                     LAAFVVMFLKNVFMNIFAPMVDKAAAANHQGKLVMLHYGTWIIYYLIIASISFIGILV
                     GSGPVNSFVHHIPQNLMNGLSAAGGLLPAVGFAMLMKLLWTNKLAVFYLLGFVLTAYL
                     KLPAVAVAALGAVICVISSQRDIELDAITRGAISKQTTFDSKESEEEDFFA"
     gene            complement(2216..2707)
                     /locus_tag="VBC28_001804"
     CDS             complement(2216..2707)
                     /locus_tag="VBC28_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003048186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEO6815764.1"
                     /translation="MITQIRVDDRLIHGQVAVVWTKELNAPLLVVANDEAAKNEITQM
                     TLKMAVPNGMKLLIRSVEESIALFKDPRATDKRIFVIVNSVKDACTIAKNITDLEAVN
                     VANVGRFDKSDPATKVKLTSSLLLNTEELEAAKELASLPDLDVFNQVLPSNTKVNLSQ
                     LVK"
     gene            complement(2725..3150)
                     /locus_tag="VBC28_001805"
     CDS             complement(2725..3150)
                     /locus_tag="VBC28_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HEO6815765.1"
                     /translation="MKRKFLIGSHGKLASGLQSSIDILTGKGQEIQTIDAYIDDSDYT
                     KSIVEFIDEIAPDEQGLIFTDLLGGSVNQKMATAVMNSGKNNIFLITNSNLATLLSLL
                     FLKPEEELTKEEIVTVINESQVQLVDLSFKAGSEDDFFD"
     gene            complement(3397..4431)
                     /locus_tag="VBC28_001806"
     CDS             complement(3397..4431)
                     /locus_tag="VBC28_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEO6815766.1"
                     /translation="MDNKQHNTSMISLLQYLFSILVILVHSGRLFSQDVIHFTFKSFL
                     GRMAVPYFLICTAFFLRGRIQQGLCNHSYFRKLIKKYSMWTIIYLPYGYFFFESLNIA
                     KIYLLPGFIVAFLYLGMSHTLWYIPAVILGWVIIQGLLKYVGTRGTFITVVVLYCIGA
                     VETYSVFIQSTKFYPLMSTYMSIFQTTRNGLFYTPVYLLAGYLLYDYFNTDLFTKSRG
                     LKYILFLLLLALENVLIYFNQGLDKNFFLLAPLCAVFLFNWSIRTSLFKEYRLSPLKQ
                     LSVYYFFLPPLFIGIVSYCLKSTSLVAHHQGKVIFVVTLALTHACALLIIKVVNKIPY
                     QKIIPLSLSK"
     gene            complement(4660..>5431)
                     /locus_tag="VBC28_001807"
     CDS             complement(4660..>5431)
                     /locus_tag="VBC28_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEO6815767.1"
                     /translation="KDQKQLGSALPLKQLKLGNFAPWNQLFEVSNSTYILIVLFTLFL
                     SAPLFSGDRAKKINPILLTTRYGRGQLTWAKLASTMTIGISTFILAYALILTVFADYF
                     GFSGWETSVQLNLYWTSPLANIMGFPVPMTISTAFVHIIVLQFVGLLFIASLNFFISS
                     ITNSPLTSFAVSTLAFFAPSFLMAMFNKGVINKLLTIFSVSTTDTSALLLKLSRNGSH
                     GFFFDSFSANEICLISLRMLIAVLLIAYTYRLMRRRSL"
BASE COUNT         1910 a         1048 c          861 g         1612 t
ORIGIN      
        1 agccttatct aaaacttcac taatgtaaat agacttacta aaaaaattac ctaaatgtga
       61 attactagca aacaaaagtt cttgattagg tgagaatact aaaagttctg tttgactttg
      121 taaatgattc cttaaatgat aatattgctt actcaaatca gcacttccta agtgtcctga
      181 gacaaatggt ttcaaactat ttttacctaa gtcatctaat aaggtatcat gtttttgaat
      241 caattgctta taactatagg aaactgaatt aacatcttta taaagaccta tctgttgagg
      301 cgctaaagaa aaaaggacga aaagtgcaat caataacaag gataaaatta aagactggcg
      361 ggaaaaatgt ttagagatat catcttgtag tcgtttctta aagcgttctt ccattctata
      421 tcctctcatt tccatctcct tttattatag caaatagcta tccaataaag atagtcaact
      481 cctttcatac aataactttc aaaagaaaaa aagataagct aaacaagtat cgtcttgttc
      541 cacttacctt taatgctagg agattattag caaataccta agagaccaag tacaatacct
      601 aacaccataa taccaaacat gatccagttg atattgactt ttttacgtag aagccagaag
      661 gctaatatcg ttaccaacag tggcactacg ccaacaaata attggtcaag gtaactttgt
      721 atggaagctg cttccttagc tcctttagct gctaccgtta agattgtctt gaatttaaca
      781 tttgaagcag tcattgagcc taccatcatt aaaccaagca tacttgaagt cttagtaaca
      841 attttaatac cacctgattc atacaatttt ttgataaacc ctgcacctac agagtaacct
      901 ccatagagac tataataatg aatcagaaaa gctggaatgt tatacaagag tagaaagaca
      961 acagctccca tggctgagcc tgtacttgct aaggaaatac cgatacctgc cgcaataacc
     1021 cttagaattc cccaaaagaa tgaatcacca acaccagata taggtcccat caaagaagcc
     1081 ttaactgcgg tgatagcact agcgtcaaaa tcaggatctt cactgttttt cttttccata
     1141 gatgcaacaa gacccataat aaaattatta atgtgcatgg tggcattaaa ccatgttgta
     1201 tgacggacca gtgcctctgc tttatcctta tcagtcttat aaaaacgatt gatgactggt
     1261 agtaaagtat aaataactcc tactgcgtgg tattgtgttg ccccagtacg actggcattc
     1321 atcgtccatg agcgccagaa gactgagcga agcattttac ggtcttcttt agtcaaatta
     1381 tcctgtgatt tcatgcgaag aagtcctcct cttctgattc tttagagtca aaggttgtct
     1441 gtttactgat cgcacctcta gtaattgcgt ctaattctat atcacgttgt gaactaatga
     1501 cgcaaataac tgctcctagg gcagcaactg caaccgctgg taattttaaa taagctgtta
     1561 aaacaaaacc aagcaagtaa aaaacagcta atttatttgt ccataataat ttcatcagca
     1621 tggcaaaacc aacagctgga aggaggcctc ccgccgcact caaaccattc atcaggtttt
     1681 gaggaatatg atggacaaaa ctattaacag gcccactacc tactaaaata ccaatgaaag
     1741 aaatagaagc gataatgaga tagtaaataa tccatgtacc atagtgaagc atcacaagtt
     1801 taccttgatg gtttgcggct gcagctttat ccaccatcgg tgcaaagata ttcatgaaga
     1861 cgtttttcaa aaacataaca acaaatgctg ccaacattcc aatcggaaca gctagagtca
     1921 ttgctgcttt ttgatcaatg tttgaaataa tggtaaaagt tgttgccatc gctgttgctg
     1981 ttactggctc tgcggcaata acacccccaa tattaacgtt tcccaaaaat agcgcttcta
     2041 gggaggctcc cattaggata ccaaccttaa tatcacctag tagcattcct gttacaagac
     2101 caactacaag tggtctctcc atcatacttt gacctgttaa gtaatttccc ccaaaacaaa
     2161 tcaatacagc taaagctgcc atcgtcgctg gcattatcat atgtcatacc tcactttatt
     2221 tgactaactg actcaagtta accttagtat ttgaaggcaa tacttggtta aagacgtcta
     2281 aatctggtaa gctggctaat tcttttgcgg cttccaactc ttctgtattt aataagagac
     2341 ttgatgttag tttgactttt gtagctggat cagacttatc aaagcgtcct acattagcga
     2401 cgtttactgc ttctaaatcg gttatatttt tagcaattgt acaggcatct ttaacagaat
     2461 tgacaatcac aaaaatgcgc ttgtcagttg cacgtggatc tttaaataga gctattgatt
     2521 cttcaactga acggattaat aatttcatgc cattaggcac agccatttta agagtcatct
     2581 gtgtgatttc attttttgca gcttcatcat tagccaccac taaaagtggt gcatttaatt
     2641 ctttagtcca tacaactgcc acttgcccgt gaatcaaacg gtcgtcaaca cgaatttgag
     2701 taatcatttt tttcctccta attattaatc gaaaaagtca tcttctgaac ccgctttaaa
     2761 gctaagatct accagctgca cttgagattc attgataacg gttacaattt cttctttagt
     2821 taattcctct tcaggtttca aaaatagtaa tgacaacaag gtcgctaaat tgctgttcgt
     2881 tattaaaaaa atattatttt tcccactatt cattacagca gtcgccattt tctgattaac
     2941 gctgccacct aacaaatctg taaaaataag cccctgttca tctggtgcaa tctcatcaat
     3001 aaattctaca atagacttag tatagtcact atcatcaatg taagcatcta ttgtctgaat
     3061 ctcttgtccc ttgcctgtca gaatatcaat agaactctgc aacccactcg caagttttcc
     3121 gtgactgccg attaggaatt tccgcttcat ctttctcctc ctatttatta cattacccta
     3181 aattagttct gaaagtttta tcaatacttt gaataagata acctttacta tcattctaac
     3241 aaatgaaagc ggtttaaaaa tggactattc tactctatat gtggactatt ctacataatt
     3301 aatatttaaa aacagaacaa taaaaaggtc atgtatccta atagtttaca caacctttac
     3361 atcactataa ctttaaactt tctcttatat gcatcactat ttcgaaagag ataatggaat
     3421 aattttttga tagggtatct tattaacaac ctttattatg agtagagcac aagcatgtgt
     3481 taatgccaag gtcaccacaa aaatcacttt tccttgatgg tgtgcaacta aggaagttga
     3541 ttttaaacag taagacacta tgccaataaa aagaggggga aggaagaaat aatagacact
     3601 caactgtttt aaaggactta aacgatattc cttaaacagt gatgtacgta tactccagtt
     3661 aaataagaat actgcacata aaggagctaa taagaagaaa tttttatcca acccttgatt
     3721 aaaataaata agaacattct ctaaagctag aagtaaaagg aagagaatat acttaagacc
     3781 acgcgatttg gtaaataggt cagtattaaa ataatcatag agtaaatatc ctgctagaag
     3841 ataaactgga gtgtaaaata atccgtttcg tgttgtctga aaaattgaca tataagtgga
     3901 catcagggga taaaattttg ttgactgtat aaagacagaa taggtctcta ctgctccaat
     3961 acaatagagt acaacaactg tgataaatgt ccccctagtt cctacatact tcaataatcc
     4021 ttgaataatg acccaaccta gaataacagc agggatgtac caaagggtat gtgacatacc
     4081 taaatacaaa aatgccacaa taaaccccgg caacaaatag atcttcgcta tatttagaga
     4141 ttcaaaaaag aaataaccat aaggcaaata aatgatagtc cacatgctat actttttaat
     4201 cagtttacga aagtaggagt gattacacaa accttgctgt atacgacctc tcaaaaagaa
     4261 agcagtacaa atcaaaaaat aaggaacagc catccgacct aggaaacttt taaaggtaaa
     4321 atgaatgaca tcctgactaa ataacctccc actatgaact aaaatgacca gtatggaaaa
     4381 aaggtattgt agcaaactaa tcattgatgt attatgctgc ttattatcca cgcactgact
     4441 tctcttcatc cttaacatta ctttctataa gttatcatta ctatgatacc aatctcttca
     4501 gaagaatatc tcaattatcc caaaaagtca ttgaaacgcc ttaaatggta tctgataatt
     4561 attgttaata ccttatcaac catattagga aataaaaaag ctgatttcgc aaaagaaaat
     4621 cagcttagaa taatttcttg ttcagataac cagtgggaac tataaacttc ttcgtcgcat
     4681 taaacgatag gtataggcta tcaataacac tgcaattaac atacgtagac taatcagaca
     4741 gatttcatta gcagaaaaac tatcaaagaa aaagccatga ctaccattac gtgataattt
     4801 taaaagtaaa gctgaagtgt ctgtcgttga tactgagaaa atagttaata acttgtttat
     4861 cacaccttta ttaaacatag ccattaaaaa gctaggagca aaaaaggcaa gcgttgaaac
     4921 agcaaaagat gttaatggcg aatttgtgat acttgatata aagaaattaa gactggctat
     4981 aaaaaggagt ccaacaaatt gtaagacaat aatatgaaca aaagcagttg atattgtcat
     5041 aggtacagga aatcccataa tattagccaa aggtgatgtc cagtataaat tcagttgaac
     5101 gcttgtctcc caaccactaa aaccaaaata atctgcaaat accgtcagaa tgagggcata
     5161 tgctaggata aatgtggaaa taccgatggt cattgtacta gctaacttag cccaggtcaa
     5221 ctgaccacgt ccataccgag tcgtcagtag tataggatta atcttcttag ccctatctcc
     5281 tgaaaaaaga ggagctgata agaatagagt aaataagaca attaaaatat aagtactatt
     5341 acttacttcg aaaagttgat tccaaggagc aaagttacct agctttagtt gttttagtgg
     5401 tagcgcagaa cccaactgtt tttgatcttt a
//
LOCUS       DATRMA010000096         5385 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.097, whole genome shotgun sequence.
ACCESSION   DATRMA010000096 DATRMA010000000
VERSION     DATRMA010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5385
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.097"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            124..1497
                     /locus_tag="VBC28_001808"
     CDS             124..1497
                     /locus_tag="VBC28_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000914222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO6815768.1"
                     /translation="MLKKLFAPPQKRERLSAEEIKHKYPRYRVQVLISIFVGYMGYYF
                     VRNTTSILSGILNMSATEIGIITCASYIAYGLSKFISGLISDESNSKIFLPVGLFLTG
                     LVNVLIGVIPSVITSIWLFTIMYLINGWLQGMGYPPGARTLVYWYDNKERIKYATIWN
                     LSHNFGGAIAPILTGVGLALAGNDSLNQARAAYWFPGVVACLLAVLVYFLQEDTPESI
                     GLPPIEEYHKEQYTNVVDSSDILEEPEVLGMGEIIKKYILPNTKLMWASLYSIFVYIL
                     RYGIVSWTPKFLATSVQDGGKGITATAGMGGFSLFEIGGIIGMLTAGYLSAKVFKNSK
                     PLTNVAFLVVAILLLAAYWFIPAGPQYMALDFIILLGLGASIYGPVMMVGLYAMELVP
                     KAAAGAASGLTGTFSYVGGATIATLAIGIIIDHFGWGVAFIIFGISGFAAIVCTLLSR
                     DKSLEYW"
     gene            complement(1655..1960)
                     /locus_tag="VBC28_001809"
     CDS             complement(1655..1960)
                     /locus_tag="VBC28_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6815769.1"
                     /translation="MTYELCLEYGTYPLRPVDAWADEINTAPAFITEDKKLLELLEEV
                     NTLFHELFLTIECSFHYIGHDFPEKRAKITQIYHVIIEHLSIHYPEYDIKIESLLMG"
     gene            2123..3109
                     /locus_tag="VBC28_001810"
     CDS             2123..3109
                     /locus_tag="VBC28_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HEO6815770.1"
                     /translation="MSEKIRVLLYYKYVSIENAEEYAAKHLEFCKSIGLKGRILIADE
                     GINGTVSGDYETTQKYMDWVHSDERFADLWFKIDEENQQAFRKMFVRYKKEIVHLGLE
                     DNNFDSDINPLETTGEYLNPKQFKEALLDEDTVVLDTRNDYEYDLGHFRGAIRPDIRN
                     FRELPQWVRDNKDKFMEKRVVVYCTGGVRCEKFSGWMVREGFKDVGQLHGGIATYGKD
                     PEVQGELWDGAMYVFDDRISVPINHVNPTVISKDYFDGTPCERYVNCANPFCNKQIFA
                     SEENEAKYVRGCSPECRAHERNRYVQENGLSRQEWAERLEAIGESLPQVANV"
     gene            complement(3146..4273)
                     /locus_tag="VBC28_001811"
     CDS             complement(3146..4273)
                     /locus_tag="VBC28_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000683737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEO6815771.1"
                     /translation="MKHYQTIIIGAGAAGIGFGSAMQRLGLTNFLIIEKGHIGESFLR
                     WPRTTQFITPSFTTNGFGFPDLNAVIPDTSPAFSFEKEHLSGVEYARYLQLVAAHYNL
                     PIQNETSVLSIDKRDSLFVIKTSKGDFSADYLIMATGEFQNPNTIDIKGADLGMHYGQ
                     VDNFHIKSDNPFIIIGGNESACDALTHLVYLGNQVELYTDTFGRKESNPDPSISLSPL
                     TKERLKHIQDHKKEYYSISEGKKAIEIKQIGKQYQVTFDDGSTAESFHKPILSTGFLN
                     TCHLIDGIALFEYDKNQLPIVTEDDESTIVNNCFLIGPSLRQRDTIFCYIYKFRQRFV
                     PVINHIAQREGIILDEDTLEFFKENQMYLDNLACCDVNCDC"
     gene            4377..>5385
                     /locus_tag="VBC28_001812"
     CDS             4377..>5385
                     /locus_tag="VBC28_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001932930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEO6815772.1"
                     /translation="MFLLLCILSMWLMIFGVAFYYFGSLHQSLTSRIIYQFVLTVLLT
                     TTAWFMGAYFLAFEGHFKTVFQFQEADGKQIVNCLFQLCFALYAVVMLIGSIIDRVQT
                     KRLLLAVVSWLFLVYTPLAYLIWNSEGVFAKMGVLDFSGGMIVHLSAGLSSYILAHVI
                     GKSEHQHNKVKNDSLFLGMILITFGWFGFNMGPVGEWNSQAIMILLNTIFAIIGGGLA
                     WTLAAKWNGEEEKTGSLLNGIIVGLVTSTAGVGYLLTWQLLAVTFFASLFTYFVTDYV
                     AKAFAIDDVVSSFGMNGIGGLLGSLGVGLFKLSHMPVQLLALATTILLSIIMTYIISK
                     AI"
BASE COUNT         1608 a          788 c         1097 g         1892 t
ORIGIN      
        1 atagtattat ccacatttaa aaaaataaga taaaatgata aaaaataaat tattaattaa
       61 ttgacagtgt gaattcatag ttgtataatt aaacacatct aaaaatcgga aaggtaattt
      121 gctatgttga aaaaactatt tgcaccccct cagaaaagag aacgtttatc agctgaggaa
      181 attaagcata aatatcctcg ttatcgtgta caagtcctta tttctatttt tgtaggttat
      241 atgggatact attttgttcg taatacgact tcaattttat ctggtatcct taatatgtct
      301 gctactgaga ttggtattat tacctgtgct agctacattg cttatggtct ctctaaattt
      361 atttctggtt tgatttcaga tgaatcgaat tctaaaattt tccttccagt tggtttgttt
      421 cttacaggac tagttaatgt gttaatcggt gttattccat ctgtaattac tagtatctgg
      481 ctctttacta tcatgtatct tattaatggt tggttacaag ggatgggata ccctcctgga
      541 gcgcgtacat tagtctattg gtatgataat aaagaaagaa ttaaatatgc taccatttgg
      601 aatttatctc acaattttgg tggagcaata gcgccgattt taactggtgt aggtctagct
      661 cttgctggta atgattctct taatcaagcg cgtgctgctt attggtttcc aggggtggtg
      721 gcttgtcttt tggctgttct tgtttacttt ttacaagaag atactccaga atctatcgga
      781 cttccgccta tagaagagta tcataaagag caatatacta atgttgtgga ttcaagtgac
      841 attttagaag agcctgaagt tttgggtatg ggcgaaatta taaaaaaata tattctacca
      901 aatactaaac tgatgtgggc atccttgtat tcaatatttg tatatatttt acgttacggt
      961 attgtctcat ggactcctaa gtttttagcg acgagtgtgc aagatggtgg aaaaggtatt
     1021 actgcaacag ctggtatggg tggtttttca ctttttgaaa taggtggtat tattgggatg
     1081 ctaacagcag gatatctatc tgccaaagtg tttaaaaact ctaaaccttt aaccaatgta
     1141 gccttcttag ttgtagcaat cttattacta gctgcctatt ggtttattcc agcaggacct
     1201 caatatatgg cattagactt cattatttta ctaggtttgg gggcatcaat ctacggtcct
     1261 gttatgatgg ttggcctgta tgctatggag ttagttccaa aagcagcagc tggagcagct
     1321 tcaggattga ctggcacatt tagttatgtc ggaggagcaa cgatcgctac tctagctatt
     1381 ggtattatta ttgaccactt tggttgggga gtagcattta taatatttgg tatttctgga
     1441 tttgcggcta ttgtctgtac cttgctttca agagataaga gtctagagta ttggtgatat
     1501 tatttggtaa aagtatcatt taatatttag tgagataagt tatgaaaaaa ggcctcttga
     1561 aagagacctt ttttgtttta gataatctat ttgttgaagt aattagtgat atcgattgtt
     1621 tttaatagat agtttctacc tagttaaaat aaaactaccc cattaataat gattcaattt
     1681 taatgtcata ttcagggtag tgtatgctta aatgttcgat aatcacatga taaatttgtg
     1741 tgatttttgc tcttttttct gggaagtcat gaccaatata gtggaaggaa cattctatcg
     1801 ttaaaaagag ctcatggaat aaggtattga cttcttctaa aagttctaaa agttttttat
     1861 cttcagtaat aaacgcaggt gcagtattga tttcatcagc ccaagcatct acaggtctaa
     1921 gtggataagt accgtattct agacataatt cataagtcat aaaagtcctc ctagtctttt
     1981 actattttaa catatgtgtg ttcttaagaa gaataatttg tctttaaagc aaagaatatg
     2041 tcctaagcgt attttttgct atgttgtaag gagtcgttat ataatgaagt atactaactt
     2101 attttgcaga tgaggagaaa aaatgtcaga aaaaatcaga gttttactct attataaata
     2161 tgtttcaatt gaaaatgcgg aagagtacgc tgcgaaacat ctagaatttt gtaaatctat
     2221 tgggcttaaa ggtcgtattt tgattgcgga tgaggggatt aatggaactg tttctggaga
     2281 ttatgaaaca actcagaagt atatggattg ggtccacagc gatgagcgtt ttgctgatct
     2341 ttggttcaag attgacgagg aaaatcaaca agctttccgt aaaatgtttg ttcgttataa
     2401 gaaagagatt gtgcaccttg ggttagaaga taataacttt gatagcgata ttaatccttt
     2461 ggaaacaaca ggtgagtatt taaatcctaa acaatttaaa gaagccttgc ttgatgaaga
     2521 tactgttgtt ttagatacgc gtaatgatta tgagtatgac ttaggtcact tccgtggagc
     2581 aattcgtcct gatattcgca atttccgtga gttaccacaa tgggtacgtg acaacaaaga
     2641 taagtttatg gaaaaacgtg ttgttgtgta ctgtaccggt ggtgttcgtt gtgagaaatt
     2701 ctcgggttgg atggttcgag aaggttttaa agatgttgga caattacacg gtggtattgc
     2761 aacatatgga aaagatccgg aagttcaagg tgaactttgg gatggtgcga tgtatgtttt
     2821 tgatgatcgt atctcggtgc caattaatca tgttaatcca actgttatta gtaaagatta
     2881 ttttgatgga acaccttgtg aacgttatgt caattgtgca aatccgtttt gtaacaaaca
     2941 aatttttgct tcagaagaaa acgaagcgaa gtatgttcga gggtgctcgc ctgaatgtcg
     3001 tgcacatgaa cgtaatcgct atgtgcaaga aaatggttta tcacgtcaag aatgggcaga
     3061 acgtttagaa gctattggag aatccttacc tcaagtagca aatgtttaat attaaaaaat
     3121 cgctagcaat aaagctagcg attttttagc aatcacaatt gacatcgcaa caagctaggt
     3181 tatcaaggta catttgattt tctttaaaga attcaagggt atcttcgtct aatataatcc
     3241 cttctcgttg tgcgatatga ttaataacag ggacaaagcg ctgtctgaat ttataaatat
     3301 agcagaaaat agtatctctt tgtcgtaagc ttggaccaat taagaaacag ttattaacga
     3361 tagtagattc gtcatcttca gtaactattg gcaactgatt tttatcgtat tcaaataaag
     3421 cgattccgtc aatgagatga caggtattta gaaaacctgt ggaaagaata ggtttatgga
     3481 aagactccgc agtactaccg tcatcaaaag taacttgata ttgttttcca atttgtttaa
     3541 tctcaatggc tttcttgcct tcagagatac tataatattc ttttttgtgg tcttgtatat
     3601 gttttaaacg ttctttagtt aggggggata aagagatgct tggatcggga ttgctctctt
     3661 tacgaccaaa cgtatctgta tataattcga cttgatttcc caaatatact agatgtgtta
     3721 gtgcatcaca agcactttcg tttccaccaa tgatgataaa tggattgtca gatttgatat
     3781 ggaaattatc aacttgacca tagtgcattc ctagatctgc acctttaata tcaatggtat
     3841 ttggattttg aaattctcct gttgccataa taagataatc agcagaaaaa tctccctttg
     3901 aagttttaat aacaaaaagg ctatctcgtt tatcaataga tagaacagat gtttcgtttt
     3961 ggatagggag attataatga gcagcaacta attgtaagta gcgtgcatat tccactccag
     4021 aaagatgttc tttttcaaat gagaaagcag gagaagtatc tggaataaca gcgtttagat
     4081 ctggaaaacc aaagccattg gtagtgaagg aaggtgtgat aaattgtgtt gttctgggcc
     4141 atcttaaaaa agattctcca atgtgccctt tttcaatgat aagaaaattt gtaagtccta
     4201 atcgttgcat ggcagaacca aaaccgattc ctgcagcgcc tgcaccgata attatggttt
     4261 gataatgttt catgtcttct ccttgacaaa aataaatgtg taagataaaa tacttaacca
     4321 gttaacaata atacagttta acaaataaaa gagtgtttgt aaagaaagga ctatttgtgt
     4381 ttttactatt atgtatttta tcgatgtggc tcatgatttt tggggttgca ttctattact
     4441 ttgggtcgct acatcaatca ttgacttcaa ggattattta tcagtttgta cttactgttt
     4501 tgctaacgac aacagcttgg ttcatgggtg cctatttttt agcatttgaa ggtcatttta
     4561 aaactgtctt tcaattccaa gaagcagatg gtaaacaaat tgtaaattgt ttatttcaac
     4621 tttgtttcgc cctttacgca gtagtaatgc ttattgggtc aattattgat agagttcaga
     4681 cgaagcgact tttattagct gttgtttctt ggcttttctt ggtgtatacg cccttagctt
     4741 atttaatttg gaattcagag ggtgtttttg caaagatggg agtacttgat ttttcaggcg
     4801 gaatgatcgt tcatttatct gcagggcttt cttcgtatat tttagctcat gttattggca
     4861 agagtgaaca tcagcacaat aaggttaaaa atgatagtct ctttttagga atgatactta
     4921 ttacatttgg ttggtttgga tttaatatgg gtccagttgg tgagtggaat tcacaagcca
     4981 ttatgatact tttaaatact atatttgcta ttataggtgg tggtttggcg tggacattgg
     5041 ctgctaagtg gaatggtgag gaagagaaaa caggaagctt attgaatggc attattgttg
     5101 gtcttgtgac gagtacagca ggagttgggt atctcttaac ttggcaatta ttagctgtaa
     5161 cattctttgc atccctcttc acctactttg taacggatta cgttgcgaaa gcttttgcca
     5221 ttgatgatgt cgtttcctcc tttggaatga atggtattgg gggattgttg ggaagtttag
     5281 gagttggtct ctttaaatta tcacatatgc cagttcaact gttagcatta gctaccacaa
     5341 ttcttttgtc aattataatg acctatatca tatctaaagc gattt
//
LOCUS       DATRMA010000097         5265 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.098, whole genome shotgun sequence.
ACCESSION   DATRMA010000097 DATRMA010000000
VERSION     DATRMA010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5265
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.098"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            <1..1055
                     /locus_tag="VBC28_001813"
     CDS             <1..1055
                     /locus_tag="VBC28_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEO6815773.1"
                     /translation="TKIFNEEIKHFKFQKPMPYPTYFRYFIPEVIHEDKVLYLDCDMI
                     ITSDLTSIFTLDISKYGVAAVRDDLLEEYDGKEDYFNSGLLLINNIFWREQGISQRLL
                     DYTRENQGALQYHDQDVLNDVLCDNWLELDETYNYHTGADMLYNLFQQSERQLNRRKD
                     LPKVIHYTATKPWKYLETSVRWRDIWWEYNRLEWRDIFTRWQVNDGVDVSLKKVLQPI
                     HRAFIFTYSDSIEGIERLAKDLPNWQFHIAAYTQISEKLKQLEMHSNIKLYPLVYRFK
                     LNELVENSDIYLDINHGDADTQVLEQVKKLGKDILSLSTTYHGQGKQFERVTDIIDYL
                     KNERFKEHIRKEYCEK"
     gene            1045..2229
                     /locus_tag="VBC28_001814"
     CDS             1045..2229
                     /locus_tag="VBC28_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 8"
                     /protein_id="HEO6815774.1"
                     /translation="MKNNVICLAGDNKSLNQIQTVIKSILCHNDRVSIYILNQDIASE
                     WFRNIQRRLLNSHSCIFDIKLFDDTFKEFKTPRAHITYMAYARYYIPQLIDAEKVLYL
                     DIDTLVVDNLDKLFEIELGDYPIAAILDGDGIHFNSGVMLINSLYWMRYRVTEKLLEI
                     TERELDNGIFGDQGVLNLLFDNNWLKLEDKYNAQVGNDLGAFYENWQGYFDRNFESPT
                     IIHYCTHDKPWNTFSSSRFRETWWQYEQLDWNEVFNFETYLLPEPTFEKHFFTFTTSV
                     DLLYIEELVELFPNSCFHIAAWTSFGPRLLKLATNSNVRLYPSITSPLFEQLMTKANY
                     YLDISTSWKEIQFCQKAIEKSIPILSFNEAVTFEYRELSHCFINLEDMRQFLCKNGGN
                     SD"
     gene            2222..3097
                     /locus_tag="VBC28_001815"
     CDS             2222..3097
                     /locus_tag="VBC28_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000130208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEO6815775.1"
                     /translation="MTDKLVSVIIPVYNAAPYLEGCVNTILGQTYQVFEILLIDDGST
                     DTSASICDQLSLRDNRIRVFHIENGGASKARNFGLARISPESQFVTFVDSDDWVKENY
                     LEVLLAQQEKYNADIVISNYYIYRETEDIFGYYITDKDFVIEEISAQTAIDRQVHWHL
                     NSSVFIVIWGKLYRRELFDTITFPIDKVFEDELVSVLLFIKSKKTILVNGSYYGYRIR
                     PNSIMTSAFSSKRAEDLIYIFDSKLSEIILSGLDSTATVERYFWLMRDYKQRLEQENL
                     QKTIIYKVISNRLSR"
     gene            3158..4354
                     /locus_tag="VBC28_001816"
     CDS             3158..4354
                     /locus_tag="VBC28_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP_1767 family glycosyltransferase"
                     /protein_id="HEO6815776.1"
                     /translation="MRVIKVKTISETLDYISKHTASVARFGDGEIDIIAGNSIPYQVY
                     DSELAKRLERLLNRQSNEKLVICVSDVFDNLGRYNDFSQQFWRDHLYQYNDLYQKNCQ
                     AEWYGSTFISRPYMDLLDKSLSVTYFEQLKALWKNKDILIVEGETSRSGVGNDLFNDA
                     NTISRIICPSKDAFSSFYEIKETILEHSRGKLILLMLGPTAKLLAEDLSQLGHQAIDL
                     GHIDSEYEWFKMGAVSKVKLSHKHTAEHNFDEDITLIEDNSYENQIVSKIGTSDRQYA
                     LTVTLTDDIWELEHLLQRCPNTDFHIAAPVYCSDRLKQLVGYPNYYLHEAITEEQFEV
                     LLLNSDIYLDINHGEEVWNVVDRCITEGKTIYGIRRARKDNQYISFERTMDDFEYLCD
                     TIKQNR"
     gene            4478..>5265
                     /gene="secY2"
                     /locus_tag="VBC28_001817"
     CDS             4478..>5265
                     /gene="secY2"
                     /locus_tag="VBC28_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein translocase subunit
                     SecY2"
                     /protein_id="HEO6815777.1"
                     /translation="MKLLYIFEKNIILRKILITFSLIIIFLLGRYVPIPGVLISAYKG
                     QDNNFATLYSTVTGGNLSQVGVFSLGIGPMMTTMILLRLFTIGKYSSGVSQKVQQFRQ
                     NVVMLVIAIIQGLAITISFQYHNGFSLTKLLLATMILVTGAYIIFWIGNLNAEYGFGG
                     MTILVVVGMLVGQFNNIPLIFELFQDGYQLAIILFLLWTLVAMYLMITFERSEYRIPV
                     MRTSIHNRLVDDAYMPIKVNASGGMAFMYVYTLLMFPQYIIILL"
BASE COUNT         1762 a          733 c          975 g         1795 t
ORIGIN      
        1 acactaaaat ttttaatgaa gaaattaaac attttaaatt tcaaaaaccg atgccctatc
       61 ctacttactt tcgatacttt atacctgaag ttattcatga agataaggta ctatatttag
      121 actgtgatat gattatcacc agtgatttaa catcgatatt tactcttgat atttcaaaat
      181 atggtgtagc ggctgttcga gatgatttgc tcgaagaata tgatggtaaa gaagattatt
      241 ttaattcagg tttgctatta attaataata ttttttggcg agaacaaggg attagtcagc
      301 gactgttaga ttatactaga gaaaaccaag gggctcttca atatcatgac caggatgttt
      361 tgaatgatgt actgtgcgat aattggctag aacttgatga aacttataac tatcatacag
      421 gagcagatat gttatataac ctattccaac agtctgaacg tcaattaaat cgcaggaaag
      481 atttacctaa agttattcat tatacagcga caaaaccatg gaaatacctt gaaacatctg
      541 ttcgttggcg cgatatatgg tgggaatata atagacttga atggcgcgat atttttacaa
      601 gatggcaagt caatgatggt gtagatgttt cattgaagaa agtgttacaa cctatccatc
      661 gagctttcat ttttacttac tcagattcaa ttgaaggaat tgagaggttg gctaaggatt
      721 taccaaactg gcaatttcat attgctgcct atactcaaat ttcagaaaaa ttgaaacaat
      781 tggaaatgca ttcaaatata aaactttatc cacttgttta tcgtttcaaa ttaaatgagc
      841 tagttgaaaa ttcagatatt taccttgata ttaatcatgg tgatgcagat actcaggttc
      901 tggagcaagt gaagaaatta ggtaaagata ttttatccct ttctacaact tatcatggtc
      961 aaggcaaaca atttgagaga gttacggata ttattgatta tttaaagaat gaacgattta
     1021 aagaacatat tagaaaggaa tattgtgaaa aataacgtta tttgtttggc aggtgataat
     1081 aaatctttaa atcagatcca aacagttata aaatcgattt tatgtcacaa tgatagagta
     1141 agtatttata ttttaaatca agatatagct tcagaatggt ttagaaatat tcaaagacgt
     1201 ttattaaata gtcatagttg tatttttgat ataaaactct ttgatgatac ttttaaggaa
     1261 tttaaaacgc cccgtgccca tattacctat atggcttatg caaggtacta cattccgcaa
     1321 ctgattgatg cagagaaagt tctttattta gatattgata ctttggttgt tgataactta
     1381 gataagttgt ttgagataga attaggggac tacccgatag cagctatctt agatggagac
     1441 ggtattcatt ttaactcagg tgttatgcta ataaactcgc tttactggat gagatatcga
     1501 gtaacagaaa aacttctgga aataacagaa cgagaattgg ataatggaat ttttggagac
     1561 caaggggttc tcaatcttct ttttgataat aattggctta agttagaaga taagtataat
     1621 gctcaagttg gaaatgattt aggtgccttt tatgaaaatt ggcaagggta ttttgatcgt
     1681 aactttgaat cgcctactat tatccattac tgtactcatg ataaaccatg gaatactttt
     1741 tcttcttcaa ggtttaggga gacatggtgg caatatgagc aactagattg gaatgaggtg
     1801 tttaattttg aaacgtatct gttacctgag ccaacatttg aaaaacactt ctttactttc
     1861 acaacgtccg tggatttgct ttatattgaa gaattggttg aactattccc caatagttgc
     1921 ttccatatag ctgcttggac ctcttttggc ccaagattac ttaagttagc cacaaactct
     1981 aacgttcgtc tgtatccttc aataaccagc ccactttttg aacaactaat gactaaagca
     2041 aattattacc ttgatatctc aacttcttgg aaggaaatac aattttgtca aaaagctatt
     2101 gagaaatcaa ttccgatatt gagttttaat gaagcagtga catttgaata tagggagtta
     2161 agtcattgct ttattaattt ggaggatatg cgacagtttt tatgtaaaaa tggaggcaat
     2221 agtgactgat aaattagttt ctgttattat tccagtctat aatgcagcac catatttaga
     2281 ggggtgtgta aataccattt taggacaaac ataccaagtt tttgaaattc tactgataga
     2341 tgacggttct acagatacat ctgctagtat ctgcgatcaa ttatccctaa gagataaccg
     2401 tattagagtt ttccatatcg aaaatggcgg tgcttctaaa gctagaaact ttggattagc
     2461 acgaattagt ccagagagcc aatttgttac gtttgttgat tcggacgatt gggtaaagga
     2521 aaattatttg gaagtactgc tagctcagca agaaaaatat aatgctgata ttgttatcag
     2581 taattactat atctacagag aaacagaaga tatttttggt tactacatca ctgacaaaga
     2641 ttttgttatc gaagaaatct ctgctcaaac agctattgat agacaagtgc attggcattt
     2701 aaatagttca gtattcatcg ttatttgggg gaaattatat cgaagagaac tatttgatac
     2761 catcaccttc ccaatagata aagtatttga agatgagttg gtaagtgttt tactttttat
     2821 aaaaagtaaa aaaacaattt tagttaatgg aagctattat ggctatagaa ttcgtccaaa
     2881 tagcattatg acatcggcgt tttcatccaa aagagcagag gatcttattt atatttttga
     2941 tagtaaattg tcggagatta tactatcagg attggattca actgcaactg tagagagata
     3001 tttttggtta atgcgtgact ataaacagcg cttagaacaa gaaaatttac agaaaacaat
     3061 aatatataaa gtgatatcaa atcggttatc aaggtaacag taaataccat tttatttgtc
     3121 gtaatcttgc aaagagagtt gaaaagaatt aatagttatg agagttatta aagtaaaaac
     3181 aatttcggaa actttagatt atatttctaa acataccgct tcggtagcgc gttttggtga
     3241 tggagaaatt gatattattg cggggaattc tattccttac caggtttatg attctgaact
     3301 tgctaagcga ttagaacgtt tattaaatcg acagagtaat gagaagttag ttatctgtgt
     3361 aagtgatgtt tttgataatt taggtagata caacgatttt tcacaacaat tttggaggga
     3421 tcatctatat cagtataatg atttgtacca aaaaaattgt caggctgaat ggtatggttc
     3481 aacttttatt tctagaccct atatggattt actggataag agtttgtcag taacgtactt
     3541 tgaacagcta aaagcattat ggaaaaataa agatatttta attgtcgagg gtgagacttc
     3601 tcgttcaggc gttggcaatg acttatttaa tgatgccaat actatttcgc gtatcatttg
     3661 tccttcgaaa gatgcatttt catcttttta tgaaatcaag gaaactatac ttgaacatag
     3721 tagaggtaag ctaattttac ttatgcttgg tccaactgca aagctattag ctgaagattt
     3781 atcgcagcta ggacatcaag ctattgattt aggtcatatc gattcagaat atgagtggtt
     3841 taagatggga gcagtttcta aggtgaaact ttctcataaa catacggcag aacataattt
     3901 tgacgaagat attactttaa tagaagacaa tagttacgaa aatcaaattg tatcaaaaat
     3961 tggtacatca gatagacagt acgctttgac agtgacgtta acagatgata tatgggagct
     4021 agaacattta ttacaaagat gtcctaatac tgattttcat attgctgccc cagtttattg
     4081 ttcggataga cttaagcagt tagtaggtta tccaaattat tacctacatg aagctattac
     4141 tgaagagcaa ttcgaagtac tcctattaaa tagcgatata tatttagata ttaatcatgg
     4201 tgaagaagtt tggaatgttg ttgatagatg tataactgag ggtaaaacta tatatggaat
     4261 cagacgggca agaaaagata atcaatatat ttcttttgag cgaaccatgg atgattttga
     4321 atatttgtgt gacactatta aacaaaatag atagtcaaga agagtaatgg ttacttgaat
     4381 attgaaatca attttcttga ttttaaaatt aacgaagttg acgatataaa gttacttatc
     4441 ttgaagagaa caaattgata gagaaagaga aattccattg aaattactat atatttttga
     4501 aaaaaatatt attcttcgta aaatattgat aacattttca ttaataatca tttttttact
     4561 tggtcgctat gttccaatac ctggtgtctt gatttctgct tataagggac aagacaataa
     4621 ctttgcgacg ctttattcaa cagtaactgg tggtaatttg tctcaggttg gggtcttttc
     4681 attgggtatt ggtcctatga tgaccacaat gattttacta agactattta cgattggtaa
     4741 atactctagt ggagtatctc aaaaagttca acagtttcgc caaaatgtgg tgatgcttgt
     4801 catagcaatt attcaagggc tagccataac catatctttt caatatcata atggtttttc
     4861 tctgaccaaa ttattactcg cgacaatgat acttgtgact ggtgcatata ttattttctg
     4921 gattggtaat ctaaatgcgg aatacgggtt tggtggaatg actattttag tggtggtagg
     4981 tatgcttgtt gggcaattta ataatattcc tcttattttt gaattattcc aagatggtta
     5041 tcaacttgct atcatcttat ttcttttgtg gacactagta gcaatgtact taatgataac
     5101 ttttgaacgt tcagaatatc gtataccagt aatgcgtact tcaatccata atcgtctcgt
     5161 tgatgatgcc tacatgccaa taaaagtaaa tgcttcgggg gggatggcat ttatgtatgt
     5221 ttatacctta ctaatgttcc cccagtatat tattatttta ctaag
//
LOCUS       DATRMA010000098         4995 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.099, whole genome shotgun sequence.
ACCESSION   DATRMA010000098 DATRMA010000000
VERSION     DATRMA010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4995
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.099"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            14..84
                     /locus_tag="VBC28_001818"
     tRNA            14..84
                     /locus_tag="VBC28_001818"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:46..48,aa:Gly,seq:tcc)
     gene            119..192
                     /locus_tag="VBC28_001819"
     tRNA            119..192
                     /locus_tag="VBC28_001819"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:153..155,aa:Ile,seq:gat)
     gene            206..293
                     /locus_tag="VBC28_001820"
     tRNA            206..293
                     /locus_tag="VBC28_001820"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:240..242,aa:Ser,seq:gct)
     gene            529..1872
                     /locus_tag="VBC28_001821"
     CDS             529..1872
                     /locus_tag="VBC28_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEO6815778.1"
                     /translation="MKKRILSAVLVSGVTLGTAAVTVNADDFDSKIAATDSVINTLSG
                     QQAAAQNQVTAIKGQVGALESQQSELEAQNAQLEAVSQQLGQEIQTLSNKIVARNESL
                     KKQVRSAQKGNLTNYINTILNSKSVSDAVNRVVAIREVVSANEKMLAQQEADKAALEA
                     KQIENQNAINTVAANKQAIENNKAALATQRAQLEAAQLELSAQLTTVQNEKASLIQAK
                     AQAEEAARKAAEAQAAAEAKAQAEAKAQAESVAKAQAAAQVESATAPTETVQTQPRTE
                     IKPSNLTATSSATTVATTTATATNEPKVTQPSVVTKAVEAPKAVVSSTPRAVSKPVVR
                     SYDSSNTYPMGQCTWGAKSMASWVGNYWGNANQWGASARAAGYSVGTTPRVGAVAVWP
                     YDGGGYGHVAVVTSVANNSSIQVMESNYAGNMSIGNYRGSFNPSASGSVYYIYPN"
     gene            1996..2964
                     /locus_tag="VBC28_001822"
     CDS             1996..2964
                     /locus_tag="VBC28_001822"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000122447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEO6815779.1"
                     /translation="MSYSNLKLFALSSNKELAKKVSQTIGIPLGQSTVRQFSDGEIQV
                     NIEESIRGHHVFILQSTSSPVNDNLMEILIMVDALKRASAESVSVVMPYYGYARQDRK
                     ARSREPITSKLVANMLEVAGVDRLLTVDLHAAQIQGFFDIPVDHLMGAPLIADYFDRQ
                     GLVGDDVVVVSPDHGGVTRARKLAQCLKTPIAIIDKRRSVTKMNTSEVMNIIGNIKGK
                     KCILIDDMIDTAGTICHAADALAEAGATAVYASCTHPVLSGPALDNIQNSAIEKLIVL
                     DTIYLPEERLIDKIEQISIAELIGEAIIRIHEKRPLSPLFEMNKLK"
     gene            3072..4247
                     /locus_tag="VBC28_001823"
     CDS             3072..4247
                     /locus_tag="VBC28_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEO6815780.1"
                     /translation="MTLEKRFNKYLDRIEVSLIRQFDQSISDIPGMVKLTLGEPDFTT
                     PDHVKEAAKSAIDANQSYYTGMSGLLALRQAAADFAKDKYNLTYNPDCEILVTIGATE
                     ALSASLIAILEAGDVVLLPAPAYPGYEPIVNLVGADIVEIDTRENDFRLTPEMLETAI
                     IQQGEKLKAVLLNYPTNPTGITYSRQEIAALAEVLKKYDIFVISDEVYSELTYTGQQH
                     VSIAEYLPNQTILINGLSKSHAMTGWRVGLVYAPEAFIAQIIKSHQYMVTAASTISQF
                     AGVEALSVGKNDTLPMRQGYIKRRDYIIDKMSKLGFKIIKPSGAFYIFAKIPDSYPQD
                     SFKFCQDFAYQQAVAIIPGVAFGKYGEGYIRLSYAASMEVIETAMARLKVFMESYEG"
     gene            4237..>4995
                     /gene="recO"
                     /locus_tag="VBC28_001824"
     CDS             4237..>4995
                     /gene="recO"
                     /locus_tag="VBC28_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEO6815781.1"
                     /translation="MRVSQTYGLVLYNRNYREDDKLVKIFTETEGKRMFFVKHASKSK
                     FNAVLQPLTIAHFILKINDNGLSYIDDYKEVLAFQETNSDLFKLSYASYITSLADVAI
                     SDNVADAQLFIFLKKTLELIEDGLDYEILTNIFEVQLLERFGVALNFHDCVFCHRVGL
                     PFDFSHKYSGLLCPNHYYKDERRNHLDPNMLYLINRFQSIQFDDLQTISVKPEMKLKI
                     RQFLDMIYDEYVGIHLKSKKFIDDLSSWGSIMKSD"
BASE COUNT         1570 a          798 c         1008 g         1619 t
ORIGIN      
        1 atataagttt ttagcgggtg tagtttagtg gtaaaactac agccttccaa gctgttgtcg
       61 cgagttcgat tctcgtcacc cgcttacttt acataaaagt aaaaccaagc atttgcttgg
      121 gcgcgtagct caggtggtta gagcgcacgc ctgataagcg tgaggtcggt ggttcgagtc
      181 cactcgtgcc cattaatatt aatttggaga attactcaag aggctgaaga ggacggtttg
      241 ctaaatcgtt aggtcgggta actggcgcaa gggttcgaat cccttattct ccgtaaatat
      301 tataatttta agagtaatta tactcttttt ttatttctct tttagtttca ctatggagta
      361 attatattga tagagtgaat agaagaaggt tatcgttttc tagatttgat tatggttcat
      421 ttagaaatag ttgatttcaa atataaatgt aacaatgacg taatataatt tcgtgaattt
      481 tttggtaaaa tatttaatgt cttatcgtaa aggagtagtt taataaaaat gaaaaaaaga
      541 atattatcag cagtccttgt gagtggagta actcttggaa ctgctgccgt aacagtaaac
      601 gctgatgact ttgactcgaa aattgctgct actgattcag taatcaatac tttatcaggg
      661 caacaagcag cagcacaaaa ccaggtgact gctattaaag gacaagttgg tgctttagag
      721 tctcaacaat cagaattaga agcgcaaaat gctcaacttg aggctgtatc tcagcagtta
      781 ggtcaagaga ttcaaactct ttctaataaa attgttgcac gtaacgaatc tttgaaaaag
      841 caagttcgta gtgctcaaaa gggtaacttg actaactata ttaatactat tttaaattcg
      901 aaatcagtat ctgatgctgt taatcgtgtt gttgctattc gtgaggttgt ttcagctaat
      961 gaaaagatgt tagcacaaca agaggctgac aaagcggcct tagaagctaa acaaattgaa
     1021 aatcaaaatg ctataaatac tgtagcagct aacaagcaag ctatcgaaaa taataaagca
     1081 gccttagcaa cacaaagagc acaattagaa gcagctcaat tagagttatc tgctcaattg
     1141 actacagtcc aaaatgaaaa agcatcttta attcaggcaa aagcacaagc tgaggaagct
     1201 gcgcgtaagg cagctgaagc acaagcagca gctgaagcga aagcacaagc tgaagcgaaa
     1261 gcacaagctg agtctgtagc aaaagcacag gcagcagcac aagttgagtc tgccacagct
     1321 cctactgaaa cagttcaaac acaaccgaga actgaaataa agccttctaa tcttacggct
     1381 acttcttcgg caactactgt tgctacaact acagctacag ctactaatga gccaaaagtt
     1441 actcaacctt ctgtagtaac taaagcagtt gaagcaccta aagcagttgt ttcttcaaca
     1501 cctagagcgg tatcaaaacc tgttgtcaga agttatgatt caagtaacac atacccaatg
     1561 ggacaatgta cttggggagc taagtcaatg gcttcttggg ttggtaacta ttggggaaat
     1621 gctaatcagt ggggtgctag tgcacgtgca gctggttatt ctgtaggaac tacccctcgt
     1681 gttggtgcag tagctgtttg gccatatgat ggtggtggtt acgggcacgt tgcagtagta
     1741 acgtcagtgg ctaacaattc atctatccaa gttatggaat caaactatgc tggaaatatg
     1801 agtattggca attaccgtgg ttcatttaac cctagtgctt ctggctctgt ttactatatt
     1861 tacccaaatt aatgaatact aaggagatct ttcgtggtct cctttttgtt tattaattag
     1921 gtatggttta ttttggattg aaaaattgtg taaaaagcgt taaaatggta taggactaaa
     1981 gttttggagg aaatcatgtc ttattctaat ttaaaattgt ttgcactgtc atcaaacaaa
     2041 gaactagcga aaaaagtgtc tcagacgatt ggaattccgc taggtcaatc gacagttcgc
     2101 caattttctg atggtgagat tcaagttaat attgaagagt ctattcgtgg ccatcatgtt
     2161 tttattttac aatcaacaag ctcaccagtt aatgataatt tgatggaaat tttaattatg
     2221 gttgacgctt taaagcgtgc tagtgcggag tctgttagtg tggtaatgcc ttactatggg
     2281 tatgctcgtc aagatcgcaa ggcacgttct cgtgaaccta ttacttcgaa actcgttgcg
     2341 aacatgctag aagttgctgg tgttgatcgt cttttaacag ttgatttaca tgcagctcaa
     2401 attcagggat tctttgatat tcccgttgat caccttatgg gggctcccct tattgcagat
     2461 tactttgatc gtcagggatt ggttggtgat gatgttgttg tcgttagtcc tgatcatggt
     2521 ggcgtaacac gtgcacgtaa gctggcacag tgtttaaaga caccgattgc tatcattgat
     2581 aagcgccgta gtgttactaa aatgaataca tctgaagtaa tgaatattat tggtaatatc
     2641 aaaggtaaaa aatgtatttt aattgatgat atgattgata ctgcaggtac tatttgccat
     2701 gctgcagacg ctttagctga agcaggggca acagcggttt atgcttcatg tactcaccct
     2761 gttttatcag ggccggcttt ggataatatt caaaattcag ctattgaaaa attaattgtg
     2821 ttagatacga tttacctccc tgaagaacgt ttgattgata agattgaaca aatttcaatt
     2881 gcggaattga ttggtgaagc tattatccgt attcacgaga aacgtccttt atcaccatta
     2941 tttgaaatga ataaactaaa atgacaaaaa acctgttatt aacaggtttt ttatcattta
     3001 atgacggaaa actggacaca aattattttt cttgtataat taaatatatt atttcttatc
     3061 aggaggttat gatgacatta gagaaacgat ttaataagta cttagataga atagaggtct
     3121 ccttaattcg tcagtttgat caatctattt cagatatacc tggaatggta aaattaactc
     3181 ttggagaacc agattttacc actcctgatc atgttaaaga agcagctaaa tcagctattg
     3241 atgcgaatca atcgtattac actggtatga gcggtttatt agctctaagg caagctgctg
     3301 cagattttgc aaaggataaa tataatctta cttataatcc tgattgtgag atcttggtta
     3361 cgataggtgc cacagaagct ctgtctgcat ctcttatagc tattttagaa gctggtgatg
     3421 ttgttctatt acctgccccc gcttatccag gatatgagcc aatagttaac cttgtaggag
     3481 ctgatatcgt agaaattgat actagagaaa atgactttag actaactcct gaaatgttag
     3541 aaacggctat tatccaacaa ggtgaaaaat taaaggctgt tttacttaat tatcctacta
     3601 accctacagg tattacctat tcaaggcaag aaattgctgc attagctgaa gtccttaaaa
     3661 aatatgatat ttttgttatt agtgatgaag tttattctga gttaacttac actggtcagc
     3721 agcatgtttc aattgcagaa tatttaccta accaaactat cttaattaat ggcttatcaa
     3781 aatcacatgc tatgactggt tggagagttg gattggtgta tgctcctgaa gcatttattg
     3841 ctcaaatcat taaaagtcat caatacatgg tgacagctgc tagtactatt tcgcaatttg
     3901 ctggggtaga agccttatct gtgggtaaaa atgatacgtt acctatgcgt caaggatata
     3961 ttaaacgtag agattatatc attgataaaa tgtctaaact tggtttcaaa ataattaaac
     4021 cgagtggcgc tttttatatc tttgctaaaa tacctgatag ctatccacag gattctttca
     4081 agttttgtca ggattttgct tatcaacaag ctgtagctat tattccagga gtggcttttg
     4141 gtaaatatgg tgaaggatat atacgtcttt cttatgctgc tagtatggaa gttattgaaa
     4201 cagcaatggc tagattgaaa gtttttatgg aatcatatga gggttagtca aacatacggt
     4261 ctcgttttgt ataatcgtaa ttatcgtgaa gatgataaat tagttaaaat ctttacggag
     4321 actgagggga aaaggatgtt ttttgtaaag catgcttcaa agtcaaaatt caatgctgtg
     4381 ctccaacctt tgactattgc tcattttatt ttaaaaatta atgataatgg tctttcttat
     4441 attgatgatt ataaagaagt tttagcattt caagaaacca attcagactt gtttaagttg
     4501 tcatatgcga gttatattac ttctttggct gatgtggcta ttagtgataa tgtagcggat
     4561 gctcaattat ttattttcct aaagaaaacg ttagaattga ttgaggacgg tttagattat
     4621 gagattctaa cgaatatttt tgaagtacaa ctactagaga ggttcggtgt tgctttaaat
     4681 tttcatgatt gtgttttttg tcatagggta ggattacctt ttgatttttc acacaaatat
     4741 tctggattat tatgtccaaa ccattattat aaagacgaga gaagaaacca cctagatcct
     4801 aatatgctgt acttaatcaa tcgttttcag tcaattcaat ttgatgattt acaaacaatt
     4861 tctgtgaaac ctgagatgaa acttaaaatt cgtcaatttt tggacatgat ttacgatgaa
     4921 tatgtaggga ttcatcttaa aagtaaaaaa tttattgatg atttgtctag ttggggaagt
     4981 attatgaaat cagat
//
LOCUS       DATRMA010000099         4879 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.100, whole genome shotgun sequence.
ACCESSION   DATRMA010000099 DATRMA010000000
VERSION     DATRMA010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4879
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.100"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            61..285
                     /locus_tag="VBC28_001825"
     CDS             61..285
                     /locus_tag="VBC28_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin NrdH"
                     /protein_id="HEO6815782.1"
                     /translation="MAAITVFSKNNCMQCKMTKKFLDQHGADFEEINIDEKPEKIEYV
                     KNLGFSAAPVIEAGNVVFSGFQPSKLKELV"
     gene            363..2522
                     /gene="nrdE"
                     /locus_tag="VBC28_001826"
     CDS             363..2522
                     /gene="nrdE"
                     /locus_tag="VBC28_001826"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HEO6815783.1"
                     /translation="MSLKNIGNVSYFRLNNEINRPVNGQIPLHKDKEALTAFFKENVQ
                     PNSKAFDSITDKIAYLLKYDYLEEAFLNKYRPEFIEELSTKLFDKKFRFKSFMAAYKF
                     YQQYALKTNDGEYYLESIEDRVLFNALYFADGDEELATDLALEMISQRYQPATPSFLN
                     AGRSRRGELVSCFLIQVTDDMNAIGRSINSALQLSRIGGGVGISLSNLREAGAPIKGF
                     AGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLDVFHPDIISFLSTKKENADEKVR
                     VKTLSLGITVPDKFYELARNNQEMYLFSPYSIEREYGVPFSYIDITEKYDELVANPNI
                     TKTKINARDLETEISKLQQESGYPYIINIDTANRTNPVDGKIIMSNLCSEILQVQKPS
                     LINDAQEYLEMGTDISCNLGSTNVLNMMTSPDFGKSIKTMTRALTFVTDSSNIEAVPT
                     IKNGNAQAHTFGLGAMGLHSYLAKNHIEYGSPESIEFTDIYFMLMNYWTLVESNNIAR
                     ERQTTFVGFEKSKYADGTYFDKYVSGKFVPQSDKVKSLFANHFIPEAKDWENLRYAVM
                     KDGLYHQNRLAVAPNGSISYINDCSASIHPITQRIEERQEKKIGKIYYPANGLATDTI
                     PYYTSAYDMDMRKVIDVYAAATEHVDQGLSMTLFLRSELPKELYEWKTESKQTTRDLS
                     ILRNYAFNKGVKSIYYIRTFTDDGSEVGANQCESCVI"
     gene            2725..3684
                     /gene="nrdF"
                     /locus_tag="VBC28_001827"
     CDS             2725..3684
                     /gene="nrdF"
                     /locus_tag="VBC28_001827"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HEO6815784.1"
                     /translation="MTTYYEAINWNEIEDVIDKSTWEKLTEQFWLDTRIPLSNDLDDW
                     RKLSAQEKDLVGKVFGGLTLLDTMQSETGVEAIRADVRTPHEEAVLNNIQFMESVHAK
                     SYSSIFSTLNTKSEIEEIFEWTNNNEFLQEKARIINDIYANGNALQKKVASTYLETFL
                     FYSGFFTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEDEQENFR
                     DWMYDLLYQLYENEEKYTKTLYDGVGWTEEVMTFLRYNANKALMNLGQDPLFPDTAND
                     VNPIVMNGISTGTSNHDFFSQVGNGYLLGSVEAMHDDDYNYGL"
     regulatory      3769..3867
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3925..4488
                     /gene="thiT"
                     /locus_tag="VBC28_001828"
     CDS             3925..4488
                     /gene="thiT"
                     /locus_tag="VBC28_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000042138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HEO6815785.1"
                     /translation="MSKNNNTTCLIETAIFAALAMALSMIPDFASWFTPSFGAIPLIL
                     FALRRGTKYGLFAGLIWGLLHFVLSKVYYLSLSQVFIEYILAFISMGLAGVFSAKFKD
                     ALSSSSKTKALSLALSGAILATFVRYVWHYIAGVIFWASYAPKGMSATLYSLSVNGTA
                     GLLTLFFVVISIIILVISYPSFFLPKK"
     gene            complement(4573..>4879)
                     /locus_tag="VBC28_001829"
     CDS             complement(4573..>4879)
                     /locus_tag="VBC28_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000977218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3169 family protein"
                     /protein_id="HEO6815786.1"
                     /translation="HIYFLKVYRNIRGIKMTVAPTLKELKNNVLQLDEAELESNYKMC
                     FDIVMNLSGFIFPTIYFVLFFISFVFQKVEIVAIIITTSIHIYILIKSLKAARHFYR"
BASE COUNT         1619 a          859 c          804 g         1597 t
ORIGIN      
        1 acttgctttt tccattcttt ttttatatgc tattaccatg aaaaaaatta ggaggacaac
       61 atggcagcta ttactgtttt ttcgaaaaac aactgtatgc aatgtaaaat gactaagaaa
      121 tttcttgacc agcacggcgc tgattttgaa gaaattaata ttgatgaaaa accagaaaaa
      181 attgaatatg tgaaaaatct cggcttttct gcggctccag tgattgaagc tggtaatgtt
      241 gttttttcag gtttccaacc aagcaaacta aaagaacttg tttaagcaaa ttagagtctg
      301 ggaataaagc aattaattgc tttattccca ttttgtattg ttaagttaag aggataaatt
      361 caatgagttt aaaaaatatt ggcaatgtat cttactttcg cctaaataat gaaattaacc
      421 gtcctgttaa tggacaaatc ccactccata aagataaaga agccttaact gcttttttca
      481 aagaaaacgt acagcctaat tcaaaagctt ttgactcaat cactgataaa attgcttacc
      541 tccttaagta tgattatctt gaagaagctt tcctcaataa atatcgtcct gaatttattg
      601 aagaattatc taccaaactc ttcgataaaa agtttcgttt taaatccttc atggcagcct
      661 ataagtttta tcagcagtat gcattaaaaa caaatgatgg agaatactat ttagaaagta
      721 ttgaagatcg cgttctcttt aacgctcttt attttgcaga cggtgatgaa gaactagcaa
      781 ctgacttagc tctagaaatg atttcacaac gctatcaacc tgcaacacca agttttttaa
      841 atgctggtcg aagtcgtcgc ggagaattag tctcatgttt cttaatccaa gtaactgatg
      901 atatgaatgc tattggtcgt tcgattaatt cagctcttca attatctcgt attggtggtg
      961 gggttggaat atcacttagt aaccttcgtg aggctggagc tcctatcaaa ggttttgctg
     1021 gtgctgcttc tggagtcgtt ccagtcatga aattatttga agacagtttt tcttactcca
     1081 accaacttgg acaacgccaa ggtgctggag ttgtttacct tgacgttttc catccagata
     1141 ttatttcatt cttatcaact aaaaaagaaa atgctgatga aaaagtacgt gttaaaacat
     1201 tgtcattagg gattactgta cccgataaat tctatgaact agcacgtaac aatcaagaaa
     1261 tgtacttgtt tagtccttat agtatcgaac gtgaatatgg tgttcctttc agctatattg
     1321 atattaccga aaaatacgat gaattagtcg ctaaccctaa tatcactaaa acaaaaatca
     1381 atgctcgtga tttagaaaca gagatttcta aacttcaaca agaatcaggt tatccttaca
     1441 tcatcaatat tgacactgct aatcgtacta accctgttga tggaaaaatt atcatgtcaa
     1501 atctttgctc tgagattttg caagtacaaa aaccaagtct tatcaatgat gctcaagaat
     1561 accttgaaat gggaactgat atttcttgta accttggctc aactaatgtt ttaaatatga
     1621 tgacatctcc tgattttgga aaatcaatta aaacaatgac tcgtgcttta actttcgtta
     1681 ctgactcatc aaatattgaa gctgttccaa ctattaaaaa cggaaatgct caagcacata
     1741 catttggact tggagcaatg ggactccact cctatttggc aaaaaatcat atcgaatatg
     1801 gtagtccaga atcaattgaa tttactgata tttatttcat gctaatgaac tactggacct
     1861 tagtagaatc aaacaatatt gcccgcgaac gtcaaacaac atttgtaggt tttgaaaaat
     1921 caaaatacgc tgacggtaca tactttgata aatatgtatc tggaaaattt gttccacaat
     1981 ctgacaaagt aaaatcccta tttgctaatc attttatccc cgaagctaaa gattgggaaa
     2041 accttagata cgcagttatg aaagacggac tatatcatca aaatcggctt gcagttgctc
     2101 cgaatggttc tatttcgtat atcaacgatt gttcagcatc tatccaccct attacacaac
     2161 gtattgaaga gcgtcaagag aaaaaaatcg ggaaaattta ttatccagct aatggcttag
     2221 ctacagatac tatcccttat tacacttctg cctatgatat ggatatgcgt aaagtaatag
     2281 acgtctatgc cgcagcaact gagcacgtag accaaggtct ctcaatgaca ctctttttac
     2341 gaagcgaatt accaaaggag ttgtacgagt ggaaaacaga aagtaagcag actactcgcg
     2401 acctttcaat cctacgcaac tatgctttca acaaaggtgt gaaatcaatc tactatatcc
     2461 gtacttttac agatgatggt agcgaagtcg gagctaatca atgcgaaagt tgtgtcatct
     2521 aacgaagaaa ctctacttaa aacatcagaa actagactga gataaactat ctcagtttca
     2581 tgatgttatc ttattttcta aggtattttc atctttattt ttctaaaaat cgataatact
     2641 tgaaatattg ctagatcaga attctatatt ctactcttct tccatcatca ttatataaaa
     2701 acctatttac cattggagaa aaccatgaca acttactacg aagctataaa ctggaacgaa
     2761 attgaagatg ttattgataa atcaacttgg gaaaaactaa ccgaacaatt ttggctcgat
     2821 acacgtatcc ctttatcaaa tgacttagac gattggcgca aactttctgc tcaagaaaaa
     2881 gatcttgttg gcaaggtttt tggaggctta accctacttg ataccatgca atcagaaact
     2941 ggtgttgaag ctattcgtgc cgatgttcgc acgcctcacg aagaagctgt cttaaacaat
     3001 attcaattca tggaatctgt tcacgctaaa tcttattctt caattttctc aactttaaat
     3061 actaaatcag aaattgaaga aattttcgag tggactaata ataatgagtt ccttcaagaa
     3121 aaagcacgta ttatcaatga catttatgct aatggaaatg cccttcaaaa aaaggtggct
     3181 tccacctacc tcgaaacttt ccttttttat tctggctttt tcacacctct ttactatttg
     3241 ggaaataata agttagcaaa tgttgctgaa atcattaaat taattattcg tgatgaatct
     3301 gtacatggta cttatatcgg ttacaaattc cagcttggtt ttaacgaatt accagaagat
     3361 gagcaagaga attttcgtga ttggatgtat gacctccttt atcagctgta tgaaaacgaa
     3421 gaaaaataca ccaagacact ttatgatggc gtaggatgga ctgaagaagt tatgaccttt
     3481 ttacgctaca atgctaataa agctcttatg aatttaggac aagatccttt attcccagat
     3541 acagcaaatg atgtcaaccc aattgttatg aatggtattt caacaggaac atcaaaccat
     3601 gacttcttct ctcaagtagg taatggttac ctacttggta gcgttgaagc tatgcatgat
     3661 gatgactata actatggatt ataagaaaaa gacactgatt tagtgtcttt ttctagttaa
     3721 atacaaattt cttgacaagc aaaccctttc aagatagaat gttagagtaa atacacaagg
     3781 gagtgccttg agctgagatt gcagatatgc aaaatcctct aacctgatct cgttaggacg
     3841 agcgtaggaa ttgtggtttg aattggttaa acatgacaaa tgacaaaaga atctcctttg
     3901 ctgtataaaa aaaggagatt ttttatgtca aaaaataaca acactacctg tcttattgaa
     3961 actgctattt ttgcagcgct tgctatggct ttatcaatga ttcctgattt tgcgtcatgg
     4021 ttcactccct cttttggagc aattcctctg atactttttg cattacgtcg tggtacaaaa
     4081 tacggtctat ttgctggctt aatttggggc ttattgcact ttgttctttc caaagtatat
     4141 taccttagtt tatcccaagt ttttatagaa tacatcttag catttataag tatgggactt
     4201 gcaggcgtat tttctgctaa atttaaagat gcacttagca gcagctctaa aactaaagct
     4261 ttatcattag cattatctgg agcaatatta gcaacatttg ttcgttatgt ttggcattat
     4321 atcgctggtg ttattttttg ggctagttat gcaccaaaag gtatgtctgc tacattatac
     4381 tcattaagcg tcaatggtac tgctggtttg ttaactctat tttttgtagt cattagtatt
     4441 attattttag ttatcagcta ccctagcttc tttttaccta agaaatagaa aaaataaaag
     4501 gactgatttt cagagaatca atccttttat tttacactaa tataagtttt ttgataaaaa
     4561 atctagctac tactatctat aaaaatgacg tgctgctttt aaagacttaa tgagaatgta
     4621 gatatgaata gatgttgtta taataattgc tacaatctcc actttttgaa aaacaaatga
     4681 gataaagaat aatacaaaat agatcgtagg gaaaataaaa ccacttaaat tcatgacaat
     4741 atcaaaacac atcttataat ttgattccaa ctctgcttcg tccaattgta agacattgtt
     4801 tttcaactct tttaaagttg gagcgactgt catttttata cctcggatat tccgatatac
     4861 ctttaaaaaa tatatatgt
//
LOCUS       DATRMA010000100         4805 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.101, whole genome shotgun sequence.
ACCESSION   DATRMA010000100 DATRMA010000000
VERSION     DATRMA010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4805
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.101"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            29..1577
                     /locus_tag="VBC28_001830"
     rRNA            29..1577
                     /locus_tag="VBC28_001830"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            1632..1704
                     /locus_tag="VBC28_001831"
     tRNA            1632..1704
                     /locus_tag="VBC28_001831"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1665..1667,aa:Ala,seq:tgc)
     gene            1859..4761
                     /locus_tag="VBC28_001832"
     rRNA            1859..4761
                     /locus_tag="VBC28_001832"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT         1346 a          978 c         1401 g         1080 t
ORIGIN      
        1 cgagacagaa aagagcaaag ctcaaacttt taatgagagt ttgatcctgg ctcaggacga
       61 acgctggcgg cgtgcctaat acatgcaagt agaacgctga ggtttggtgt ttacactaga
      121 ctgatgagtt gcgaacgggt gagtaacgcg taggtaacct gcctcatagc gggggataac
      181 tattggaaac gatagctaat accgcataag agtaattaac acatgttagt tatttaaaag
      241 gagcaattgc ttcactgtga gatggacctg cgttgtatta gctagttggt gaggtaaagg
      301 ctcaccaagg cgacgataca tagccgacct gagagggtga tcggccacac tgggactgag
      361 acacggccca gactcctacg ggaggcagca gtagggaatc ttcggcaatg gacggaagtc
      421 tgaccgagca acgccgcgtg agtgaagaag gttttcggat cgtaaagctc tgttgttaga
      481 gaagaacgtt ggtaggagtg gaaaatctac caagtgacgg taactaacca gaaagggacg
      541 gctaactacg tgccagcagc cgcggtaata cgtaggtccc gagcgttgtc cggatttatt
      601 gggcgtaaag cgagcgcagg cggttcttta agtctgaagt taaaggcagt ggcttaacca
      661 ttgtacgctt tggaaactgg aggacttgag tgcagaaggg gagagtggaa ttccatgtgt
      721 agcggtgaaa tgcgtagata tatggaggaa caccggtggc gaaagcggct ctctggtctg
      781 taactgacgc tgaggctcga aagcgtgggg agcaaacagg attagatacc ctggtagtcc
      841 acgccgtaaa cgatgagtgc taggtgttag gccctttccg gggcttagtg ccgcagctaa
      901 cgcattaagc actccgcctg gggagtacga ccgcaaggtt gaaactcaaa ggaattgacg
      961 ggggcccgca caagcggtgg agcatgtggt ttaattcgaa gcaacgcgaa gaaccttacc
     1021 aggtcttgac atccttctga ccggcctaga gataggcttt ctcttcggag cagaagtgac
     1081 aggtggtgca tggttgtcgt cagctcgtgt cgtgagatgt tgggttaagt cccgcaacga
     1141 gcgcaacccc tattgttagt tgccatcatt aagttgggca ctctagcgag actgccggta
     1201 ataaaccgga ggaaggtggg gatgacgtca aatcatcatg ccccttatga cctgggctac
     1261 acacgtgcta caatggttgg tacaacgagt cgcaagccgg tgacggcaag ctaatctctt
     1321 aaagccaatc tcagttcgga ttgtaggctg caactcgcct acatgaagtc ggaatcgcta
     1381 gtaatcgcgg atcagcacgc cgcggtgaat acgttcccgg gccttgtaca caccgcccgt
     1441 cacaccacga gagtttgtaa cacccgaagt cggtgaggta accttttagg agccagccgc
     1501 ctaaggtggg atagatgatt ggggtgaagt cgtaacaagg tagccgtatc ggaaggtgcg
     1561 gctggatcac ctcctttcta aggataagga aacctgccat ttgcgtcttg tttagttttg
     1621 agaggtcttg tggggcctta gctcagctgg gagagcgcct gctttgcacg caggaggtca
     1681 gcggttcgat cccgctaggc tccattgaat cgaaaggttc aaattgttca ttgaaaattg
     1741 aatatctata tcaaattcca cgatctagaa atagattgta gaaagtaaca agaaaataaa
     1801 ccgaaaacgc tgtgaatatt taatgagttt tctagtttta aagaaactag gttaataagg
     1861 ttaagttaat aagggcgcac ggtggatgcc ttggcactag aagccgaaga aggacgtgac
     1921 taacgacgaa atgctttggg gagctgtaag taagcgctga tccagagatg tccgaatggg
     1981 ggaacccact agttaatgac tagtatccat atctgttaag gatatgagaa ggaagacgca
     2041 gtgaactgaa acatctaagt agctgcagga agagaaagca aaagcgattg ccttagtagc
     2101 ggcgagcgaa acggcaggag ggcaaaccga agtgtttaca cttcggggtt gtaggactgc
     2161 gatataggat taatcattat agaagaatta cctgggaagg taagccaaag agagtaacag
     2221 cctcgtattt aaaatgatag attaacctta gcagtatcct gagtacggcg agacacgcga
     2281 aatctcgtcg gaatctggga ggaccatctc ccaaccctaa atactctcta gtgaccgata
     2341 gtgaaccagt accgtgaggg aaaggtgaaa agcaccccgg gaggggagtg aaatagaacc
     2401 tgaaaccgtg tgcctacaac aagttcgagc ccgttaatgg gtgagagcgt gccttttgta
     2461 gaatgaaccg gcgagttacg atatgatgcg aggttaagtt gaagagacgg agccgtaggg
     2521 aaaccgagtc ttaatagggc gtcatagtat catgttgtag acccgaaacc atgtgaccta
     2581 cccatgagca ggttgaaggt gaggtaaaac tcactggagg accgaaccag ggcacgttga
     2641 aaagtgcttg gatgacttgt gggtagcgga gaaattccaa acgaacttgg agatagctgg
     2701 ttctctccga aatagcttta gggctagcgt cgatgttaag tctcttggag gtagagcact
     2761 gtttgggtga ggggtccatc ccggattacc aatctcagat aaactccgaa tgccaacgag
     2821 atataatcgg cagtcagact gcgagtgcta agatccgtag tcgaaaggga aacagcccag
     2881 accaccagct aaggtcccaa aatatatgtt aagtggaaaa ggatgtgggg ttgcacagac
     2941 aactaggatg ttagcttaga agcagctatt cattcaaaga gtgcgtaata gctcactagt
     3001 cgagtgaccc tgcgccgaaa atgtaccggg gctaaaacat attaccgaag ctgtggatga
     3061 cacaaaagtg tcatggtagg agagcgttct atgtgtgaag aaggtgtacc gtgaggagcg
     3121 ctggaacgca tagaagtgag aatgccggta tgagtagcgc aagacaggtg agaatcctgt
     3181 ccaccgtaag actaaggttt ccaggggaag gctcgtccgc cctgggttag tcgggaccta
     3241 aggagagacc gaaaggtgta tccgatggac aacaggttga tattcctgta ctagagtata
     3301 tagtgatgga gggacgcagt aggctaacta aaccagacga ttggaagtgt ctggtcaaac
     3361 agtgaggtgt gatatgagtc aaatgcttat atctttaaca ttgagctgtg acgaggagcg
     3421 aagtttagta gcgaagttag tgatgtcaca ctgccaagaa aagcttctag cgatacatat
     3481 actctacccg taccgcaaac cgacacaggt agtcgaggcg agtagcctca ggtgagcgag
     3541 agaactctcg ttaaggaact cggcaaaatg gccccgtaac ttcgggagaa ggggcgctga
     3601 ttttagatca gccgcagtga ataggcccaa gcaactgttt atcaaaaaca cagctctctg
     3661 ctaaatcgta agatgatgta tagggggtga cgcctgcccg gtgctggaag gttaagagga
     3721 gtgcttagcg taagcgaagg tatgaattga agccccagta aacggcggcc gtaactataa
     3781 cggtcctaag gtagcgaaat tccttgtcgg gtaagttccg acccgcacga aaggcgtaat
     3841 gatttgggca ctgtctcaac gagagactcg gtgaaatttt agtacctgtg aagatgcagg
     3901 ttacccgcga caggacggaa agaccccatg gagctttact gcagtttgat attgagtatc
     3961 tgtaccacat gtacaggata ggtaggagcc tatgataacg ggacgccagt ttcgtttgag
     4021 gcgttgttgg gatactaccc ttgtgttatg gctactctaa cccagatagg caatccctat
     4081 cggagacagt gtctgacggg cagtttgact ggggcggtcg cctcctaaaa ggtaacggag
     4141 gcgcccaaag gttccctcag actggttgga aatcagtcgt agagtgtaaa ggtataaggg
     4201 agcttgactg cgagagctac aactcgagca gggacgaaag tcgggcttag tgatccggtg
     4261 gttccgtatg gaagggccat cgctcaacgg ataaaagcta ccctggggat aacaggctta
     4321 tctcccccaa gagttcacat cgacggggag gtttggcacc tcgatgtcgg ctcgtcgcat
     4381 cctggggctg tagtcggtcc caagggttgg gctgttcgcc cattaaagcg gcacgcgagc
     4441 tgggttcaga acgtcgtgag acagttcggt ccctatccgt cgcgggcgta ggaaatttga
     4501 gaggatctgc tcctagtacg agaggaccag agtggactta ccgctggtgt accagttgtc
     4561 ttgccaaagg catcgctggg tagctatgta gggaagggat aagcgctgaa agcatctaag
     4621 tgcgaagccc acctcaagat gagatttccc atgattttat atcagtaaga gccctgagag
     4681 atgatcaggt agataggtta ggagtgtaat tgtggtgaca catttagcgg actaatacta
     4741 atagctcgag gacttatcca aaaagagata ttgacaacgt tacggtttct tgttagacta
     4801 tagat
//
LOCUS       DATRMA010000101         4772 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.102, whole genome shotgun sequence.
ACCESSION   DATRMA010000101 DATRMA010000000
VERSION     DATRMA010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4772
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.102"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            <1..508
                     /locus_tag="VBC28_001833"
     CDS             <1..508
                     /locus_tag="VBC28_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HEO6815787.1"
                     /translation="YYNFNKLMIYLTHPFISDLNMPSFPSSEDGLKHFADVKYLFTLA
                     HGLFVILTFPVIYFLRRGWKQKSIFLYEGFFKIAIMLPIFIVVCAFLLGFDQFFTLFH
                     EVLFPGDSTWQFNPLTDPVIWILPETFFLHCFIIFLLIYETITIILLIIGRKHLKLRK
                     YKNKMQAL"
     gene            896..>4772
                     /gene="cas9"
                     /locus_tag="VBC28_001834"
     CDS             896..>4772
                     /gene="cas9"
                     /locus_tag="VBC28_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040090.1"
                     /note="Cas9, originally named Csn1, is the large,
                     multifunctional signature protein of type II CRISPR/Cas
                     systems. It is well known even to general audiences
                     because its RNA-guided endonuclease activity has made it a
                     popular tool for custom editing of eukaryotic genomes;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR RNA-guided endonuclease Cas9"
                     /protein_id="HEO6815788.1"
                     /translation="MNKPYSIGLDIGTNSVGWSIITDDYKVPAKKMRVLGNTDKEYIK
                     KNLIGALLFDGGNTAADRRLKRTARRRYTRRRNRILYLQEIFAEEMSKVDDSFFHRLE
                     DSFLVEEDKRGSKYPIFATLQEEKDYHEKFSTIYHLRKELADKKEKADLRLIYIALAH
                     IIKFRGHFLIEDDSFDVRNTDISKQYQDFLEIFNTTFENNDLLSQNVDVEAILTDKIS
                     KSAKKDRILAQYPNQKSTGIFAEFLKLIVGNQADFKKYFNLEDKTPLQFAKDSYDEDL
                     ENLLGQIGDEFADLFSAAKKLYDSVLLSGILTVIDLSTKAPLSASMIQRYDEHREDLK
                     QLKQFVKASLPEKYQEIFADSSKDGYAGYIEGKTNQEAFYKYLSKLLTKQEDSENFLE
                     KIKNEDFLRKQRTFDNGSIPHQVHLTELKAIIRRQSEYYPFLKENQDRIEKILTFRIP
                     YYIGPLAREKSDFAWMTRKTDDSIRPWNFEDLVDKEKSAEAFIHRMTNNDFYLPEEKV
                     LPKHSLIYEKFTVYNELTKVRYKNEQGETYFFDSNIKQEIFDGVFKEHRKVSKKKLLD
                     FLAKEYEEFRIVDVIGLDKENKAFNASLGTYHDLEKILDKDFLDNPDNESILEDIVQT
                     LTLFEDREMIKKRLENYKDLFTESQLKKLYRRHYTGWGRLSAKLINGIRDKESQKTIL
                     DYLIDDGRSNRNFMQLINDDGLSFKSIISKAQAGSHSDNLKEVVGELAGSPAIKKGIL
                     QSLKIVDELVKVMGYEPEQIVVEMARENQTTNQGRRNSRQRYKLLDDGVKNLASDLNG
                     NILKEYPTDNQALQNERLFLYYLQNGRDMYTGEALDIDNLSQYDIDHIIPQAFIKDDS
                     IDNRVLVSSAKNRGKSDDVPSLEIVKDCKVFWKKLLDAKLMSQRKYDNLTKAERGGLT
                     SDDKARFIQRQLVETRQITKHVARILDERFNNELDSKGRRIRKVKIVTLKSNLVSNFR
                     KEFGFYKIREVNNYHHAHDAYLNAVVAKAILTKYPQLEPEFVYGDYPKYNSYKTRKSA
                     TEKLFFYSNIMNFFKTKVTLADGTVVVKDDIEVNNDTGEIVWDKKKHFATVRKVLSYP
                     QNNIVKKTEIQTGGFSKESILAHGNSDKLIPRKTKDIYLDPKKYGGFDSPIVAYSVLV
                     VADIKKGKAQKLKTVTELLGITIMERSRFEKNPSAFLESKGYLNIRADKLIILPKYSL
                     FELENGRRRLLASAGELQKGNELALPTQFMKFLYLASRYNESKGKPEEIEKKQEFVNQ
                     HVSYFDDILQLINDFSKRVILADANLEKIN"
BASE COUNT         1679 a          703 c          886 g         1504 t
ORIGIN      
        1 ttattataat tttaataagt tgatgatata tctgactcat ccttttatct ctgatttaaa
       61 tatgcccagc tttccttctt cagaggatgg tttaaagcat tttgcggatg taaaatatct
      121 ttttactcta gcgcatgggc tttttgttat actaactttt ccagttattt attttttaag
      181 acgtggatgg aagcaaaaaa gtatctttct atatgaaggc tttttcaaga tagctattat
      241 gttgcctatt tttattgtag tatgtgcctt tctgttgggt tttgaccagt ttttcactct
      301 atttcatgag gtgctttttc caggagattc tacttggcaa tttaatcctc taacggatcc
      361 agtaatttgg atacttcctg aaactttttt cctccattgt ttcatcattt ttttgttaat
      421 ctatgaaaca ataactataa ttcttttgat aataggtaga aaacatttaa aattaagaaa
      481 atataaaaac aaaatgcaag ccttataata gcgcttgcat tttgtttgct atttttttat
      541 aatacaaata tgagaaatgt cggaattata acaatgacat aaaaaagcac cgaatcggtg
      601 ctcacttttt caagctgaat acggactgga ttaaaaatca ctgccttatt ttaacgtgct
      661 gtgctgtttc gaataattcc aacaaaatta ttatatgtta ttttagtgta ataatcaagt
      721 aaaaaataaa aatcagcaaa gctttaaaac ttaaaacata ctttaggata ttttaacaaa
      781 taacagatga aagataaaga taatgctttt aaaattcaaa aaaataatat ataattaaag
      841 tatgttttaa aggtgttgtt tttagaatgg tgttttaaat aaaaggataa aagttatgaa
      901 taagccatat tcaataggcc ttgacatcgg tactaattcc gtcggatgga gcattattac
      961 agatgattat aaagtacctg ctaagaagat gagagtttta gggaacactg ataaagaata
     1021 tattaagaag aatctcatag gtgctctgct ttttgatggc gggaatactg ctgcagatag
     1081 acgcttgaag cgaactgctc gtcgtcgtta tacacgtcgt agaaatcgta ttctatattt
     1141 acaagaaatt tttgcagagg aaatgagtaa agttgatgat agtttctttc atcgattaga
     1201 ggattctttt ctagttgagg aagataagag agggagcaag tatcctatct ttgcaacatt
     1261 gcaggaagag aaagattatc atgaaaaatt ttcgacaatc tatcatttga gaaaagaatt
     1321 agctgacaag aaagaaaaag cagaccttcg tcttatttat attgctctag ctcatatcat
     1381 taaatttaga gggcatttcc taattgagga tgatagcttt gatgtcagga atacagacat
     1441 ttcaaaacaa tatcaagatt ttttagaaat ctttaataca acttttgaaa ataatgattt
     1501 gttatctcaa aacgttgacg tagaggcaat actaacagat aagattagca agtctgcgaa
     1561 gaaagatcgt attttagcgc agtatcctaa ccaaaaatct actggcattt ttgcagaatt
     1621 tttgaaattg attgtcggaa atcaagctga cttcaagaaa tatttcaatt tggaggataa
     1681 aacgccgctt caattcgcta aggatagcta cgatgaagat ttagaaaatc ttcttggaca
     1741 gattggtgat gaatttgcag acttattctc agcagcgaaa aagttatatg atagtgtcct
     1801 tttgtctggc attcttacag taatcgacct cagtaccaag gcgccacttt cagcttctat
     1861 gattcagcgt tatgatgaac atagagagga cttgaaacag ttaaaacaat tcgtaaaagc
     1921 ttcattgccg gaaaaatatc aagaaatatt tgctgattca tcaaaagatg gctacgctgg
     1981 ttatattgaa ggtaaaacta atcaagaagc tttttataaa tacctgtcaa aattgttgac
     2041 caagcaagaa gatagcgaga attttcttga aaaaatcaag aatgaagatt tcttgagaaa
     2101 acaaaggacc tttgataatg gctcaattcc acaccaagtc catttgacag agctgaaagc
     2161 tattatccgc cgtcaatcag aatactatcc cttcttgaaa gagaatcaag ataggattga
     2221 aaaaatcctt acctttagaa ttccttatta tatcgggcca ctagcacgtg agaagagtga
     2281 ttttgcatgg atgactcgca aaacagatga cagtattcga ccttggaatt ttgaagactt
     2341 ggttgataaa gaaaaatctg cggaagcttt tatccatcgt atgaccaaca atgattttta
     2401 tcttcctgaa gaaaaagttt taccaaagca tagtcttatt tatgaaaaat ttacggtcta
     2461 taatgagttg actaaggtta gatataaaaa tgagcaaggt gagacttatt tttttgatag
     2521 caatattaaa caagaaatct ttgatggagt attcaaggaa catcgtaagg tatccaagaa
     2581 gaagttgcta gattttctgg ctaaagaata tgaggagttt aggatagtag atgttattgg
     2641 tctagataaa gaaaataaag ctttcaacgc ctcattggga acttaccacg atctcgaaaa
     2701 aatactagac aaagattttc tagataatcc agataatgag tctattctgg aagatatcgt
     2761 ccaaactcta acattatttg aagacagaga aatgattaag aagcgtcttg aaaactataa
     2821 agatcttttt acagagtcac aactaaaaaa actctatcgt cgtcactata ctggctgggg
     2881 acgattgtct gctaagttaa tcaatggtat tcgagataaa gagagtcaaa aaacaatctt
     2941 ggactatctt attgatgatg gtagatctaa tcgcaacttt atgcagttga taaatgatga
     3001 tggtctatct ttcaaatcaa ttatcagtaa ggcacaggct ggtagtcatt cagataatct
     3061 aaaagaagtt gtaggtgagc ttgcaggtag ccctgctatt aaaaagggaa ttctacaaag
     3121 tttgaaaatt gttgatgagc ttgttaaagt catgggatac gaacctgaac aaattgtggt
     3181 tgagatggcg cgtgagaatc aaacaacaaa tcaaggtcgt cgtaactctc gacaacgcta
     3241 taaacttctt gatgatggcg ttaagaatct agctagtgac ttgaatggca atattttgaa
     3301 agaatatcct acggataatc aagcgttgca aaatgaaaga cttttccttt actacttaca
     3361 aaacggaaga gatatgtata caggggaagc tctagatatt gacaatttaa gtcaatatga
     3421 tattgaccac attattcctc aagctttcat aaaagatgat tctattgata atcgtgtttt
     3481 ggtatcatct gctaaaaatc gtggaaagtc agatgatgtt cctagccttg aaattgtaaa
     3541 agattgtaaa gttttctgga aaaaattact tgatgctaag ttaatgagtc agcgtaagta
     3601 tgataatttg actaaggcag agcgcggagg cctaacttcc gatgataagg caagatttat
     3661 ccaacgtcag ttggttgaga cacgacaaat taccaagcat gttgcccgta tcttggatga
     3721 acgctttaat aatgagcttg atagtaaagg tagaaggatc cgcaaagtta aaattgtaac
     3781 cttgaagtca aatttggttt caaatttccg aaaagaattt ggattctata aaattcgtga
     3841 agttaacaat tatcaccatg cacatgatgc ctatcttaat gcagtagttg ctaaagctat
     3901 tctaaccaaa tatcctcagt tagagccaga atttgtctac ggcgactatc caaaatataa
     3961 tagttacaaa acgcgtaaat ccgctacaga aaagctattt ttctattcaa atattatgaa
     4021 cttctttaaa actaaggtaa ctttagcgga tggaaccgtt gttgtaaaag atgatattga
     4081 agttaataat gatacgggtg aaattgtttg ggataaaaag aaacactttg cgacagttag
     4141 aaaagtcttg tcataccctc agaacaatat cgtgaagaag acagagattc agacaggtgg
     4201 tttctctaag gaatcaatct tggcgcatgg taactcagat aagttgattc caagaaaaac
     4261 gaaggatatt tatttagatc ctaagaaata tggaggtttt gatagtccga tagtagctta
     4321 ctctgtttta gttgtagctg atatcaaaaa gggtaaagca caaaaactaa aaacagttac
     4381 ggaactttta ggaattacca tcatggagag gtccagattt gagaaaaatc catcagcttt
     4441 ccttgaatca aaaggctatt taaatattag ggctgataaa ctaattattt tgcccaagta
     4501 tagtctgttc gaattagaaa atgggcgtcg tcgattactt gctagtgctg gtgaattaca
     4561 aaaaggtaat gagctagcct taccaacaca atttatgaag ttcttatacc ttgcaagtcg
     4621 ttataatgag tcaaaaggta aaccagagga gattgagaag aaacaagaat ttgtaaatca
     4681 acatgtctct tattttgatg acatccttca attaattaat gatttttcaa aacgagttat
     4741 tctagcagat gctaatttag agaaaatcaa ta
//
LOCUS       DATRMA010000102         4723 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.103, whole genome shotgun sequence.
ACCESSION   DATRMA010000102 DATRMA010000000
VERSION     DATRMA010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4723
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.103"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            25..669
                     /locus_tag="VBC28_001835"
     CDS             25..669
                     /locus_tag="VBC28_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwaF family protein"
                     /protein_id="HEO6815789.1"
                     /translation="MPLLIHSLILSVLPFLMWLTLVNRDKPLYKTIWSILLGLQLITI
                     YTWFFWAKLPLSESLPLYHCRIGMFVVLLARPGILKDYFALLGVVGGVLAMIHPDFYP
                     YQFLHVTNIFFFIGHFALFVLSLLHLMTQSNLDKLNPKLIIQLTLLINMSLIFINLLT
                     GGNYGFMMKTPILGITNPFLNLFIVTTLLSFLVLFVKQIFQKVRMRASLKTEES"
     gene            848..2386
                     /locus_tag="VBC28_001836"
     CDS             848..2386
                     /locus_tag="VBC28_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEO6815790.1"
                     /translation="MNKSFNTKLGLVTVAVLSGIVLTSQLPVNAKAETPVMAASAQQG
                     FRFVATVVDSQTHVLPGKLVTLSEVTSGQPKIIASVKTNDAGQAIFNNLPIKRNLSVS
                     VDGQVKGYTIRTDVAGSSKAASFTATGVGTNEPTYSKKTIDITVRDQNAEPVSGRTVT
                     LKTQAGREIASLVSGDNGLTRFTDRLLDGTFYQYFVDGKKIGDIVPGESRSAYVNVAP
                     KKDYFTFTVTALDKDGLVVKGKEVTLTDITDGKAVALASLKTNDNGQAIFTNLPLSRN
                     ISVSIDGKSKGYTLRTDVAGSHKAAAFYVDGKGTKAPQFSAEAAVVTVYDANGNTIAN
                     QEVTLTNSNGTIVAKGLTEKDGKARFANKLMAGTLYNIFVNGIEMPKTALVGSDVSVF
                     LTDKQIKKEKPVTPDKLDKEMNQEKEKELLDTPKPVPPTSKPTAPIKNKKDDSPKKFT
                     KSQSSSLKSSVKAIGAKPKAMTALAKKLPKTGDQAISILTLVGFIVTGLAIMLGFLKK
                     SRSK"
     gene            2563..4107
                     /locus_tag="VBC28_001837"
     CDS             2563..4107
                     /locus_tag="VBC28_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEO6815791.1"
                     /translation="MTLQDEIKKRRTFAIISHPDAGKTTITEQLLYFGGEIREAGTVK
                     GKKSGTFAKSDWMDIEKQRGISVTSSVMQFDYAGKRVNILDTPGHEDFSEDTYRTLMA
                     VDAAVMVVDSAKGIEAQTKKLFEVVKHRNIPVFTFINKLDRDGREPLDLLEELEEVLG
                     IASYPMNWPIGMGKSFEGLYDLHNKRLELYKGDERFASIEDGDQLFANNPFYEQVKED
                     IELLQEAGNDFSEQAILDGDLTPVFFGSALTNFGVQTFLDTFLEFAPEPHGHKTTEGN
                     VIDPLAKDFSGFVFKIQANMDPRHRDRIAFVRIVSGEFERGMGVNLTRTGKGAKLSNV
                     TQFMAESRENVTNAVAGDIIGVYDTGTYQVGDTLTVGKNKFEFEPLPTFTPELFMKVS
                     AKNVMKQKSFHKGIEQLVQEGAIQLYKNYQTGEYMLGAVGQLQFEVFKHRMEGEYNAE
                     VVMTPMGKKTVRWINSDDLDERMSSSRNILAKDRFDQPVFLFENDFALRWFADKYPDV
                     KLEEKM"
     gene            4193..4573
                     /locus_tag="VBC28_001838"
     CDS             4193..4573
                     /locus_tag="VBC28_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEO6815792.1"
                     /translation="MGIFSGLMGNASQMDTDKVENQLSDILISDEQVDLAYTLIRDLI
                     VFTNYRLILVDKQGVTGKKVSYNSIPYASISRFTVETSGHFDLDAELKIWISSAIEPA
                     EVLQFKNDRNIVSIQKALATAVLK"
BASE COUNT         1542 a          718 c          939 g         1524 t
ORIGIN      
        1 aatatcagaa cagagattcc gcagatgcct ttacttatcc atagtttgat tttatctgtc
       61 ttaccttttc tgatgtggct gactttggtt aatagagata agcctttgta taaaactatt
      121 tggagtatcc ttttaggact tcagttaatt acgatttata cttggttttt ctgggcaaaa
      181 ttgcctttat ctgaaagtct tcccctttac cattgtcgaa taggcatgtt tgtcgttctc
      241 ttggcgaggc ctggaatatt aaaggattat ttcgcattgt taggagtagt aggtggagta
      301 ttggcaatga ttcacccaga cttttaccct taccagtttc tacatgtgac taatattttc
      361 ttctttattg gacattttgc tttatttgtc cttagtttac tacatctgat gacacagtcc
      421 aatttggaca aattaaatcc aaaattgatt atacaactaa cgcttctcat taatatgtct
      481 ttgattttta ttaacctctt gactgggggg aattatggct ttatgatgaa gacaccaata
      541 ctaggaataa caaatccctt tttaaacctt tttatagtga caactctatt aagttttctc
      601 gtcctatttg ttaaacaaat tttccaaaaa gtgagaatga gagctagtct taaaacagaa
      661 gaatcttaat tattttaata ttattatatt atctagctat taaaaaataa atagctggat
      721 aatgtttttc atttttagct atttttatga ttgaccgcta attattaatc tgctaagata
      781 tagtacgtaa taaaaactta accggttaat tatttttgaa atatattttt tctggaggaa
      841 aatagtaatg aataaatcat tcaataccaa attaggtttg gtaactgtag cggtattatc
      901 aggtattgta ttaacgtctc aattacctgt gaacgctaaa gctgagacac cagtaatggc
      961 agcaagtgca caacaaggtt tccgttttgt agcaaccgtt gtagatagtc agacgcatgt
     1021 cttgccggga aaattggtaa ctttgtcaga ggtgacaagt ggtcagccta aaattattgc
     1081 aagtgtgaaa acgaatgatg ccggacaagc tatttttaat aatctcccta taaaacgtaa
     1141 ccttagtgtg tctgttgatg gacaagtaaa aggatatact attcgaactg acgtagcagg
     1201 ttctagtaaa gcagctagtt ttacagcaac aggagtaggg acaaatgagc caacgtattc
     1261 aaaaaaaacc atagacatca cagtgagaga ccagaatgct gaacctgttt caggcaggac
     1321 agttacctta aaaacacaag ctggtcgtga gattgctagc ttggtttctg gtgacaatgg
     1381 attaactcgc tttactgatc gtttactaga tggaacattc taccaatatt ttgttgatgg
     1441 gaaaaaaatt ggtgatattg tacctggaga atcaagaagt gcatatgtta atgttgcacc
     1501 taaaaaagat tattttacct ttacagtcac tgctttagat aaagatggac ttgttgttaa
     1561 gggaaaagaa gtaacgttga ctgatattac tgatggtaaa gcagtagcgc ttgcttcttt
     1621 aaaaacgaat gataatggac aagctatctt taccaaccta ccattatcaa gaaatatcag
     1681 tgtttcaatt gatggaaaat caaagggcta tactcttcga actgatgtag caggctctca
     1741 taaagcggct gcattctatg tagatggtaa aggtaccaaa gcacctcaat ttagtgcaga
     1801 agcagcggtt gtaactgttt acgatgctaa tggaaatact atagcaaacc aagaagtgac
     1861 attaactaat tcaaatggga caattgttgc aaaaggtttg actgaaaaag atggcaaagc
     1921 aaggttcgcc aataaattaa tggcaggaac gttatacaat atttttgtaa atggtattga
     1981 gatgcctaaa actgcccttg ttggtagtga tgtcagtgtt tttctaacag ataaacaaat
     2041 taagaaagaa aaacctgtta ctcctgataa attggataaa gaaatgaacc aagagaagga
     2101 aaaggagctc ttagatactc caaaaccagt accacctact tcgaaaccaa cagcaccaat
     2161 taaaaataaa aaggacgatt cccctaagaa gttcacgaaa agtcaatctt ctagtttgaa
     2221 gtcaagtgtt aaggctatag gtgctaagcc aaaagctatg acagccttag ctaaaaaatt
     2281 gcctaaaact ggtgatcaag ccattagtat tttaactttg gttggtttta ttgtaacagg
     2341 gttagcgatt atgttaggct tccttaaaaa atcacgatca aaataattta ttttagaagc
     2401 ttgggtcttt agacacaggc ttctcttctt tcttcggaaa tagattgaac ttgaagaatg
     2461 ttgttaaatt cgctataata ataaagttat agacaagatt attgtttcag ttgagactac
     2521 tgaaaataat actaagaaat ggataaaagc taaggattga atatgacatt acaagacgaa
     2581 atcaaaaaac gccgtacttt tgccatcatc tctcacccgg atgctggtaa gacgactatt
     2641 actgagcaat tattatattt tggtggtgaa attagagaag cagggacagt aaaagggaaa
     2701 aaatcaggta cttttgcaaa gtccgactgg atggatattg aaaagcaacg gggtatctct
     2761 gttacttcat ctgttatgca atttgattac gcgggtaaac gtgttaatat tttggataca
     2821 cctggacatg aggatttctc ggaagataca taccggacct taatggctgt ggatgcagct
     2881 gtcatggttg tagattcggc taaaggtatt gaggctcaaa ctaaaaaact ttttgaagtt
     2941 gttaagcatc gtaatattcc ggtttttacg tttattaata aattggaccg tgatggacgt
     3001 gagccattgg atttgcttga agagttagaa gaagtactag gtattgcttc ttatccaatg
     3061 aattggccga ttggtatggg gaaatcattt gaagggcttt atgatttaca taataaacgt
     3121 ttagaactct ataagggaga cgagcgtttt gcaagtattg aagacggtga tcagctgttt
     3181 gccaataatc cattttatga acaagtcaaa gaagatattg aacttcttca agaagcaggt
     3241 aatgactttt cagaacaagc tattcttgat ggtgacctaa ctccagtatt ctttggctct
     3301 gcccttacga actttggagt tcagactttt cttgatactt tcttggagtt tgctccagaa
     3361 cctcatggtc acaagactac agaaggtaat gttattgacc ccttggctaa agatttttca
     3421 ggttttgtct ttaaaattca agctaacatg gacccacgtc atcgcgaccg tattgctttt
     3481 gttcgtattg tttctggtga atttgagcgc ggtatgggag ttaatttgac acgtactggt
     3541 aaaggtgcta agctctccaa tgttactcaa tttatggcag aatcacgtga aaatgtgacg
     3601 aatgctgttg ctggggatat tatcggggtt tatgatacag gaacttatca agttggggat
     3661 accttaacag ttgggaaaaa taaatttgaa tttgaaccat taccaacttt taccccagaa
     3721 ttatttatga aagtatcagc aaaaaatgtt atgaagcaga aatcattcca caaaggtatt
     3781 gagcaattag tacaagaagg agcaatccag ctttataaga attatcagac aggcgaatac
     3841 atgctgggtg ctgtgggtca acttcagttt gaagttttta aacatcgtat ggaaggtgaa
     3901 tacaatgctg aagttgttat gacaccaatg ggtaaaaaga ctgtacgttg gattaactca
     3961 gacgatttag acgaacgcat gtcttcaagc cgaaatattt tagcaaaaga tcgttttgac
     4021 caaccagttt tcctatttga aaatgatttt gcacttcgct ggtttgctga taagtatcct
     4081 gatgtgaagc ttgaagaaaa aatgtaaaat ttaaagtcac tcaaatttga gtgacttttt
     4141 aagtttttta gactaaaata gttataatac atagaaaaga ttggagatta acatgggaat
     4201 tttttcggga ttaatgggaa atgcttcaca aatggatacc gataaagtcg agaatcagtt
     4261 atctgatatt ctgatttcag atgaacaggt tgatttagct tataccttaa ttcgtgattt
     4321 gattgttttt actaattatc gtttaatttt agtcgataaa cagggggtga caggtaaaaa
     4381 ggtttcttat aattctatac cttatgcttc tatatcacgt tttacagtgg aaactagtgg
     4441 tcattttgat ttagatgctg agttaaagat ttggatatca tcagcaatag aaccagcaga
     4501 agtacttcag tttaagaacg accgtaacat agtgagtata cagaaagctt tggcgacagc
     4561 tgttttaaaa taaacaattt agcagtagtg atttttatca ctgcttttat tttgaagtat
     4621 agtaggcctc aagcatatat tttgaaaacg ctgtattttt ttgataagat atttttttga
     4681 ggagtattaa ataatgataa tagaattaaa gagtgttgga aaa
//
LOCUS       DATRMA010000103         4631 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.104, whole genome shotgun sequence.
ACCESSION   DATRMA010000103 DATRMA010000000
VERSION     DATRMA010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4631
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.104"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            5..78
                     /locus_tag="VBC28_001839"
     tRNA            5..78
                     /locus_tag="VBC28_001839"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39..41,aa:Asn,seq:gtt)
     gene            218..1069
                     /locus_tag="VBC28_001840"
     CDS             218..1069
                     /locus_tag="VBC28_001840"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000688173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEO6815793.1"
                     /translation="MKIFEKAPAKLNLGLDIKGRCDDGYHELAMIMVSIDLNDYVTIS
                     ELKEDCIVIDSDSSKMPLNNDNDVFKAADIIKNQYGINKGVHIRLEKSIPVCAGLGGG
                     STDAAATIRALNRLWNLQMDYDEMVAIGFKIGSDVPYCLGGGCSLVLGKGEIVKPLPT
                     LRPCWIVLVKPDFGISTKSIFRDIDCKSISRVDIDLLKSAILSSDYQLMVKSMGNSLE
                     DITITKNPVISTIKERMLNSGADVALMTGSGPTVFSMCSTEKKADRVFNSMKGFCKEV
                     YKVRLLR"
     gene            1155..1598
                     /locus_tag="VBC28_001841"
     CDS             1155..1598
                     /locus_tag="VBC28_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent MarR family transcriptional
                     regulator"
                     /protein_id="HEO6815794.1"
                     /translation="MTVLEQKLDHLVSQILLKAENQHELLFGTCQSDVKLTNTQEHIL
                     MLLSQEQLTNSDLAKKLNISQAAVTKAVKSLISQDMLKANKDSKDARITYFELSELAK
                     PIADEHTHHHDNTLGVYGRLVNHFSKDEKVVLERFLDLFSRELEG"
     gene            1601..2311
                     /locus_tag="VBC28_001842"
     CDS             1601..2311
                     /locus_tag="VBC28_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEO6815795.1"
                     /translation="MRYITVSGLTFQYDSDPVLEGVNYHLDSGEFVTLTGENGAAKST
                     LIKATLGILTPKVGTVNISKENKEGKKLRIAYLPQQIASFNAGFPSSVYEFVKSGRYP
                     RNGWFRRLTKHDEEHIRVSLEAVGMWDNRHKKIGSLSGGQKQRAVIARMFASDPDIFV
                     LDEPTTGMDAGTTEKFYELMHHNAHKHGKSVLMITHDPDEVKGYADRNIHLVRNQSLP
                     WRCFNVHTNEMEVESDAT"
     gene            2301..3113
                     /locus_tag="VBC28_001843"
     CDS             2301..3113
                     /locus_tag="VBC28_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000923271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEO6815796.1"
                     /translation="MLLDMLSYDFMQRALLAVVAISIFAPILGIFLILRRQSLMSDTL
                     SHVSLAGVALGVVLGISPTWSTIFVVTLAAVVLEYLRTVYKHYMEISTAILMSMGLAI
                     SLIVMSKAHNVGNVSLEQYLFGSIITIGKEQVIALFVIALITFILTILFIRPMYILTF
                     DEDTAFVDGLPVRTMSILFNVVTGIAIALTIPAAGALLVSTIMVLPASIAMRLGRNFK
                     TVIFLGMLIGFVGMVAGIFLSYYWETPASATITMIFIGIFLLVSLVGLLRKR"
     gene            complement(3324..4354)
                     /locus_tag="VBC28_001844"
                     /pseudo
     CDS             complement(3324..4354)
                     /locus_tag="VBC28_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767962.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
BASE COUNT         1347 a          659 c          900 g         1725 t
ORIGIN      
        1 ataatccgcc atagctcagt tggtagtagc gcatgactgt taatcatgat gtcgtaggtt
       61 cgagtcctac tggcggagta gataaaggga gactaacttt ggttagcctt tttttattgg
      121 aattattatc cttaagattg gctaacatct taattgtccg aatttgtcat ttgttatgtt
      181 ataatatgga tattctatat tttattaggg aggttttatg aaaatttttg agaaagctcc
      241 tgccaaactt aatttaggat tagatattaa aggacgatgt gacgatggtt atcatgaatt
      301 agctatgatt atggttagta ttgatcttaa tgactacgtt accatttctg agctaaagga
      361 agattgtatt gttatcgatt ctgatagtag caaaatgcct ttaaataatg ataatgatgt
      421 gtttaaagct gcagatataa tcaaaaatca atatggtatt aataaaggtg ttcatattag
      481 attagaaaaa tccattcctg tctgtgcagg actggggggt ggttctactg atgctgctgc
      541 tacaataaga gctcttaatc gattatggaa tcttcaaatg gactatgacg aaatggttgc
      601 tattggtttt aaaattggta gtgatgttcc ttattgtctt ggtgggggat gttcgttagt
      661 attaggtaaa ggtgaaattg ttaaaccctt accgacattg agaccatgtt ggatagttct
      721 ggttaagccg gattttggta tttcaactaa atccattttt agagatattg attgtaagtc
      781 tataagtaga gttgatattg atttattaaa gtctgctatt ttgtcatcag attatcagtt
      841 aatggtcaaa tctatgggaa attcactcga ggatattaca attactaaaa atccagttat
      901 aagtacaata aaagaacgta tgttaaattc tggagccgat gttgcattaa tgacaggaag
      961 tggaccaact gttttttcaa tgtgttctac agagaaaaaa gctgatcgtg tttttaatag
     1021 tatgaaaggt ttttgcaaag aagtttataa agttaggttg ttgagataaa aggttttatc
     1081 ttcttcacct atctatgatt gattcttcta tatcttcgtg atataattta ctggttaaca
     1141 aaaaagaggt atatatgaca gttttagaac aaaaattaga ccatttagtg agtcaaatct
     1201 tgcttaaagc agaaaatcaa catgaattat tgtttggtac ttgtcaaagt gatgttaaat
     1261 tgactaatac gcaagaacac attctgatgc ttttatcaca agagcagttg acaaattctg
     1321 atttagctaa gaagttaaat attagtcagg cagctgtaac taaagcagta aaaagtttaa
     1381 tttctcagga tatgttgaaa gcgaataaag attctaaaga tgctcgtatt acttattttg
     1441 agttatctga acttgctaaa cccattgcag atgaacacac acatcatcat gacaatacgt
     1501 taggggttta cggaagatta gtaaatcatt tttcaaagga cgaaaaggtc gttttagaac
     1561 gttttcttga tcttttctct agagaattag aagggtaatc atgcgatata ttacagttag
     1621 tggtcttact tttcagtacg atagcgatcc tgtgctggaa ggggttaatt accatttaga
     1681 cagtggggag tttgttacct taacaggtga aaatggggct gctaaatcaa ctttaataaa
     1741 ggctacttta ggtattttaa cgcccaaagt gggtacagtt aatatctcta aagaaaacaa
     1801 agaaggaaaa aaattacgta ttgcctattt acctcaacag attgcaagtt ttaacgctgg
     1861 ctttccatcg tcagtttatg aatttgtgaa atctggacga taccctagaa atggttggtt
     1921 tagaagactg actaaacatg atgaagaaca tattagggtt agtttagaag ctgttggtat
     1981 gtgggataat cgccataaaa agataggaag tttatctggc ggtcaaaagc agagggctgt
     2041 cattgctagg atgtttgctt ctgatccaga tatttttgta ttggatgagc caacaactgg
     2101 tatggacgca gggactactg agaaatttta tgagttaatg catcataatg ctcataagca
     2161 tggtaagtct gttttgatga ttactcacga tcctgatgaa gtaaaggggt atgctgaccg
     2221 aaatattcat cttgttagga accaatcttt accatggcgt tgtttcaatg tccatacaaa
     2281 cgaaatggag gttgaatcag atgctacttg atatgctttc ctatgatttt atgcaacgag
     2341 cgctcttagc agttgtggct attagtattt ttgctccaat tttaggcatc ttcctaattt
     2401 tacgtcgtca gagtttgatg agtgatactt tgagtcacgt ttccttagcc ggtgttgcat
     2461 taggcgttgt tttaggtatc tcgcctacat ggtcaactat tttcgttgtt actttggcgg
     2521 cagttgtatt agagtatttg cgtactgtat acaaacatta tatggaaata tcaactgcta
     2581 tcctgatgtc gatggggttg gctatatctt taatcgttat gagtaaagct cataatgttg
     2641 gtaatgtcag tcttgaacag tatttatttg gttctattat tactattggg aaggaacaag
     2701 tgattgcttt gtttgttatt gcattaatca cttttatttt aaccatccta tttattagac
     2761 caatgtatat attaacgttt gatgaggata cagcttttgt agatggattg cctgtgcgta
     2821 caatgtctat tttgttcaat gttgttacag gtattgctat tgcattaaca attcctgccg
     2881 caggtgctct attggtgtca actattatgg ttttaccagc tagtattgct atgcggttag
     2941 gtcgaaattt taaaactgtt atttttctgg gtatgttaat tggatttgtg ggaatggtag
     3001 ctggtatttt cctttcgtat tattgggaga cacctgctag tgcaacaata acgatgattt
     3061 ttattggcat tttcctatta gttagcttag taggcttgct tcgcaaacgt taaataaaaa
     3121 aatctatctt attctaggat agatttttta tttatctccc tgtttttttc gaatatattt
     3181 tatgagggat cagttgagaa attatctcga tgaggtatct actaagtttt ctggcttaag
     3241 ttgatagaag gggcaatgtg aatctatttt tgcgttttag gataaaaaat actaccggca
     3301 attttgccgg cagtaaattg taattattgt gcttcagtag tgctgtgatg tttgtgttga
     3361 tttaacgctt cttgttcagt catttgaacg atattagcgg tatttgcatt ttttatgttt
     3421 tctaaactat accaataagt atttgaacga cccttatttg caacgtagac gattctattt
     3481 tggggagcta cattatttgt atctgattca gaagtattag tgttagtatt ttcttctata
     3541 tcagatgcac tttcaatagc tgcttcgttt tcatcagcat cgctatttgg atcttcacct
     3601 tcttctgcct gtttcccatc ttctttgatt agcataccag ttttgtaatc aatttttgcg
     3661 agtggagacg tattttctaa tgtaacagtt gtgacgccgt attcatcaac ttgttctttg
     3721 ccgccgccta atttgatttc tagaagtttt ccttgtttat caattccaac atactgaagt
     3781 tcaactcggc taggtactaa attatttcct tcgtatattg gagtcgcttt gtaatcaagc
     3841 tagaaatttt tatgatttgc caaccaacta tctaatctat tttcgtagta taacatacca
     3901 tcaggattat tttcgctaaa tcctgtattt agatatttgg tcatagtaac taggttctca
     3961 ggaacattgt tcattcctga aaattgataa ccaacgaggt gacctcggtc tataagccaa
     4021 gttgttttac ccttttcagt agtaaatttg tagttgtgcc aaccggttgg attatacgtc
     4081 agtctttctc tttttatctt aggctcatct gcatccctta attgtatgtg agaatatgtt
     4141 gctctttcta agttatctaa tgaacctacc ttgatttgct tattatgagt aaacggtaag
     4201 atttttttga agaagtactt acatactgtg tctcaacatg ggatgtcgtc ttataatgtt
     4261 tagccttagc ttcaacattc tgttgtaatg ccccaacgca tagtacgaca gttaatagtg
     4321 ggagtgattt gtataattgt ttagataatt tcataaacct aaacccttct aaatgatatt
     4381 tgtgaaggcc tttcacaagt ataattttat cattttattg ggaaaaagta ataaaaaaac
     4441 ataactagtt aatttttttg gagattatgt tatttaacta gatagttatt aaagttaaat
     4501 gttatagata tactgttatt acataataat ttttatatga tttgttattt tattagattt
     4561 tttaatggat tatttcttta gctgattttg attacaaagt taaggtattc taatcatatt
     4621 ttcttgattt a
//
LOCUS       DATRMA010000104         4545 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.105, whole genome shotgun sequence.
ACCESSION   DATRMA010000104 DATRMA010000000
VERSION     DATRMA010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4545
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.105"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            170..598
                     /locus_tag="VBC28_001845"
     CDS             170..598
                     /locus_tag="VBC28_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6815797.1"
                     /translation="MKKDKYIALCLLCAIATSNLSAVTVFAQDAQTILTQATTVTDQT
                     SEKAGENSSAEATSASESVSSEATTVVPEASLPSTDLHAQPADSKTAPTETVIEGQTE
                     TNPTTSPDKQFLQQVIPKEKHRLGRKQIQANPKLQLLVSL"
     gene            604..1413
                     /locus_tag="VBC28_001846"
     CDS             604..1413
                     /locus_tag="VBC28_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6815798.1"
                     /translation="MVPSQPQVTATPQKSEVVTPAITSGIDLPDVAIPTAMASAAYVK
                     HWIGNDAYTHNLLSHRYGITAAQLDGFLQSTGITYDSSRIDGQKILDREKSSGLDARA
                     IIAIAIAESSLGTQGVATAPGANMFGFGAVDNNTTNAQNFSDDKAVIKMTQETIIQNQ
                     NTSFAIQDQKAQFLSTGNLNVAARGGVYFTDASGSGKRRAAIMESIDKWIDAHGGISE
                     ISKELLNTSSVAMMAVPTSYSVSRANQAGNYVAGTYPWGQRTWLPVWTIYG"
     gene            1349..1666
                     /locus_tag="VBC28_001847"
     CDS             1349..1666
                     /locus_tag="VBC28_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEO6815799.1"
                     /translation="MWQALTLGAKELGYQFGPFMGNGGDWKNKPGYQTTHEAKTGYAI
                     SFSPGQAGADRTYGHVAIVEDVKEDGSIPISESNVLGLGTISYRTFSAAEAAQLTYVV
                     GEK"
     gene            2061..2510
                     /locus_tag="VBC28_001848"
     CDS             2061..2510
                     /locus_tag="VBC28_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001867149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HEO6815800.1"
                     /translation="MTEQQIEYINNRSEIAGHLEAIVSEIYLIEESGKIESWFVTIPD
                     MNSFVAETNMDREQILFLLDSAHKYHIYFLFGGLASYLMTNISDVPKAIRTQAQYVLL
                     GMRVMDQSVLPKSYNSKEPYLKPDMIYIHDRRQERMLKITQEIEMEH"
     gene            2515..3084
                     /locus_tag="VBC28_001849"
     CDS             2515..3084
                     /locus_tag="VBC28_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001876044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6815801.1"
                     /translation="MKKQKLLLLIGGLLIMIMMTACKDSKIPENRTKEEYQAEQNFKP
                     FFEFLAQKDKDLSKIQKYLLLVSDSGDALDLEYFYSIQDLKKNKDLGKFETRKSQIEK
                     PGGYNELENKEVPFEYFKNNIVYPKGKPNITFDDFIIGAMDTKELKELKELKELKKLK
                     VKSYLLKHPETELKDITYELPAQSKLIKK"
     gene            3239..3772
                     /locus_tag="VBC28_001850"
     CDS             3239..3772
                     /locus_tag="VBC28_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6815802.1"
                     /translation="MGNDNVTIKLEHRVKTSADLSDYVYKYERAYTDALNNGDDIKEA
                     SESTKKELNQLIDRTDLTYSDGMYDKETGIAAIAVKDKNTGETYIAYAGTNKDADGAT
                     DFISDANIGANNSAYLKRLGDKATSFYDRVQSNGDNITVTTGHSYGDFLASRVAIELI
                     PFFINFRKVNDSFHFCY"
     gene            3864..4075
                     /locus_tag="VBC28_001851"
                     /pseudo
     CDS             3864..4075
                     /locus_tag="VBC28_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285332.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4120..4443
                     /locus_tag="VBC28_001852"
     CDS             4120..4443
                     /locus_tag="VBC28_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6815803.1"
                     /translation="MNNAAIKSLKNEVLELNPKNAFKGWGKLGRIAKAGKVLGWTGKI
                     VNIETNAKKDFFDDKTSTLDQKIKNFAIDSGVDALSGAAIGTLVGGPVGTVVGAVQGW
                     HLILL"
BASE COUNT         1642 a          742 c          897 g         1264 t
ORIGIN      
        1 tgatgaaaat gaagaggaga tgcaaaaaat atttttgaaa taaataaaag atatcaaata
       61 ccctcacttt tcagtagacg ttttaaaata gtcatctgaa gaataaaaga aaagtaattt
      121 ttaatattaa attctttaaa cactctataa actattaagg aaaaacgttt tgaaaaaaga
      181 taaatatatt gcattgtgtt tgttgtgtgc aattgccacg tcaaacttat cggctgttac
      241 ggtttttgct caggatgcac aaactatttt gacgcaagct acgactgtaa ctgatcaaac
      301 aagtgaaaaa gctggagaaa attcatcagc tgaggcgaca agcgcttctg agtcagtttc
      361 atcagaagcg acgacagtgg tacctgaggc aagtctgcct tcaactgatc tccatgccca
      421 accagcagat agcaaaacag ctcctactga gactgtaatt gagggacaaa cagaaactaa
      481 tccgacgact agtccagata aacagttcct tcaacaagtg attccaaagg agaaacacag
      541 gctgggacgc aaacagatac aagcaaaccc gaaactccag ctcctagtca gtctgtaaca
      601 ccagtggtgc caagccaacc tcaagtgaca gcaacacctc agaaatctga agttgtgaca
      661 ccagccatta cgagtggcat tgatttgcca gatgttgcca tacctacggc aatggctagc
      721 gcagcttatg tcaaacactg gataggcaat gatgcttata cacacaatct attgtctcac
      781 cgttacggca ttactgccgc acagcttgat ggattcttac agtcgacagg tatcacatac
      841 gatagcagtc gcatcgacgg tcaaaaaatc ttagaccgag agaaatcatc tggtctggat
      901 gcgcgtgcaa tcattgccat tgctatcgca gaaagttcgc ttggtacaca aggcgttgcc
      961 acagcaccag gtgctaatat gtttggtttt ggggcagttg acaacaatac aactaacgct
     1021 caaaacttta gtgatgacaa ggctgtcatt aaaatgacgc aggaaaccat catccaaaac
     1081 caaaacacca gctttgccat tcaagaccaa aaagcacaat tcttgtcaac tggcaacttg
     1141 aatgtagcgg ctcgaggtgg ggtttacttc acggatgcta gcggttctgg taaacgtcgt
     1201 gcggccatta tggagagcat tgacaagtgg attgacgcgc atggcggaat ttctgagatt
     1261 tcaaaggaac tgctcaacac gtcgtccgtc gctatgatgg cagtcccaac gagttactct
     1321 gtttcaagag ctaatcaagc gggcaactat gtggcaggca cttacccttg gggccaaaga
     1381 acttggctac cagtttggac catttatggg taatggtggc gattggaaaa ataagccggg
     1441 ctatcaaacc acacacgagg caaaaacagg ctacgccatc tcatttagcc ccggccaagc
     1501 tggtgctgat agaacttacg gccacgtggc catcgtcgaa gacgttaagg aagacggatc
     1561 tatcccgatc tccgaatcaa atgtcctagg acttggcacc atctcctacc gcaccttcag
     1621 tgcagcagaa gcagcacaat tgacttatgt agttggggag aagtagataa ttattctttg
     1681 aaaaacaaaa ggttcttagc ttgtcaaaat ctaagaccct tttatctttc ataagaactt
     1741 taaaatcaac agatttttat caccaaatgg actggaccga gaccaatagc cattccaatt
     1801 gttccagaaa cacttacctt tgaggagttt aaagcatatg aggatgttaa aaaagcaagt
     1861 gagactaatc agatttcact tggtattagc ttaaaagaag ttgataattg tagcattcca
     1921 ctttcgaaat tcaaacagtt actttttact tctgacaatc cagataattt gtctaacttg
     1981 atgcgcttct tagttaaatc tgtttcattg attaaagata gtaagtttat cttttagaca
     2041 ctgcttctga ttatgatgat ttgacagaac aacaaattga atatatcaat aatcgaagtg
     2101 aaatagcagg gcatttagaa gctattgttt cagaaatcta tttaatagaa gaatctggta
     2161 aaatagaatc atggtttgtt actattcctg atatgaatag ttttgtggca gaaacaaata
     2221 tggatagaga acagatttta ttcttacttg actcggctca caaatatcat atttatttct
     2281 tatttggtgg tttagcatca taccttatga ctaatatctc agatgttcca aaagctatta
     2341 gaacacaggc acagtatgtt ctcttaggaa tgagagttat ggatcagtca gtattaccga
     2401 aatcatataa ttcaaaagaa ccttatttga aaccagatat gatttatatt catgatagaa
     2461 gacaagaaag aatgcttaaa atcactcaag aaatagaaat ggagcattga gcatatgaaa
     2521 aaacaaaaac tattactgct tattggaggc ttattaataa tgataatgat gacagcatgt
     2581 aaggattcaa aaatcccaga aaaccgcaca aaggaagagt accaagctga acaaaatttt
     2641 aaaccgtttt ttgagttttt agcacaaaaa gataaagatt tgagcaaaat acaaaaatac
     2701 ttactattag tatcggattc aggtgatgca ttagatttag aatatttcta tagtattcaa
     2761 gatttaaaaa aaaataagga tttagggaag tttgaaacaa gaaaaagtca aatagaaaag
     2821 ccgggtggct ataatgagtt agaaaataaa gaggtcccat ttgaatattt taaaaataat
     2881 atagtttatc caaaaggaaa accgaatatt acatttgatg actttattat cggagcaatg
     2941 gatactaaag aattaaaaga attaaaagaa ttaaaagaat taaaaaaatt aaaagtaaaa
     3001 agttatttat taaaacatcc ggaaactgag ttgaaagata taacatatga attgccggca
     3061 cagtcgaagc ttattaaaaa atgagagata aatttaaaat atctgatatt aaggaagcag
     3121 atattacttt tacaaaaagt ggaaaaaata attttgacta tacactaagg ataaaaacta
     3181 aaaacaagag atactatttg ggtgagacct atagttttag aaaataaacg gagaagttat
     3241 gggaaatgat aatgtaacta taaaactaga acatcgggtt aaaacatcag cagatttatc
     3301 agattatgta tacaaatatg agagggctta tacagatgct ttaaataatg gtgatgacat
     3361 aaaagaagca agtgaaagta ctaaaaaaga gttaaatcaa ctaattgata gaactgattt
     3421 aacttatagt gacggtatgt atgacaaaga gactggtatt gcagctattg ctgtaaaaga
     3481 taaaaatact ggtgagactt atatagctta tgcgggtact aataaggatg ctgatggggc
     3541 aaccgacttt atttcagatg caaatatcgg tgctaataac tctgcttatt taaaacgatt
     3601 aggagacaaa gctactagct tttatgatcg tgttcaaagt aatggcgata atattactgt
     3661 cacaacaggt cattcttatg gtgactttct cgcttctaga gtggcaattg agctaattcc
     3721 attctttatt aactttagaa aagttaatga ttcatttcac ttttgttatt agcttgtgat
     3781 cacttataaa gtgacagcta gttcatgaaa tctcttagga gttttctggc attaaaggta
     3841 acctatgatt taaaaaatat ctcatggaat tcaatgcgga atcctcaaca gtcttttgtg
     3901 gactaacaga agttcaggat gtcgtttcgg caggccttaa ctattctcag aaggttttgg
     3961 aaatttcaat ggtacatttg atacctcagg taagggatta gaatggacaa aaacaattca
     4021 aacaggtatg aaaaaacgag aagaagatgc taaacagttg agaaaaaagc tttagaagat
     4081 tcaaataagc tcagaaaaat tgcgtcaaaa ggagttactt tgaataatgc agcaataaaa
     4141 tcccttaaaa atgaagtcct tgaattaaat cccaaaaatg cttttaaagg atggggaaaa
     4201 ttaggaagaa ttgctaaagc tggtaaagta ttaggttgga ctgggaaaat agtcaatata
     4261 gaaactaatg ccaaaaaaga cttttttgat gataagactt ctacactaga tcaaaaaatc
     4321 aaaaactttg ctatagattc aggtgtagat gcattgtccg gtgcagcaat aggaactttg
     4381 gtaggaggac ctgtgggaac cgttgttgga gcagtgcagg gctggcattt gattttgctt
     4441 tgaattttaa agttaaggag ttgggtaatg aatcaattac aggttttgct aaaaaaggat
     4501 taaaagattt atttggaggg taaaattttt gaaacagagc aacca
//
LOCUS       DATRMA010000105         4465 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.106, whole genome shotgun sequence.
ACCESSION   DATRMA010000105 DATRMA010000000
VERSION     DATRMA010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4465
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.106"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            <1..73
                     /locus_tag="VBC28_001853"
     CDS             <1..73
                     /locus_tag="VBC28_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001127191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEO6815804.1"
                     /translation="DTDYLTPQKIATVIGESLVNLSN"
     gene            130..1503
                     /locus_tag="VBC28_001854"
     CDS             130..1503
                     /locus_tag="VBC28_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000089330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEO6815805.1"
                     /translation="MSRNNYGQLNHHEELRYNYLLKNIHYLNEREKMEFQYLHYKKTA
                     VRPQRRTESPPTNSYYEEPYSDSYYQDDDFYSEPQLTSQGLPIYQEERAPKKKKQRAR
                     KEKQRVKVMAPFPPKAITPPRKKKKFKGFLKFIGIILLIVLSGMVFMFVKGMRDVNNG
                     KSHYSPAIIEDFKGKDAVDGTNILILGSDKRVSERSTDARTDTIMVANVGNKDNKVKM
                     VSFMRDLLVNIPNYSTEGYYDMKLNASFNLGEQDNHKGAEYVRQTLKNHFDIDIKYYV
                     MVDFETFADAIDTLFPNGVKINAKFGLVGGQSADSVKVPDDLRMKNGVVPSQKIKVGI
                     QYMDGRTLLNYARFRKDDDGDFGRTQRQQQVMRAIVSQIKDPRRLFTGSAAIGKAYAL
                     TSSNLSYSFVLTDGIPILSDAKNGIKQMTIPREGDWVDDYDQYGGQGLTIDFAKYKKI
                     LKKMGLR"
     gene            1604..2959
                     /gene="rlmD"
                     /locus_tag="VBC28_001855"
     CDS             1604..2959
                     /gene="rlmD"
                     /locus_tag="VBC28_001855"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000902645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEO6815806.1"
                     /translation="MLHKNDIIETEISDISHEGMGIAKVDGFVFFVENALPGEIIKMR
                     VLKLRKRIGYGKVEEYLTTSPHRNEGLDYTYLRTGIADLGHLTYEQQLLFKQKQVADN
                     LYKIAHISDVLVEPTLGMTIPLAYRNKAQVPVRRVDGQLETGFFRKNSHTLVSIEDYL
                     IQEKEIDALINFTRDLLRKFDVKPYDEEQQSGLIRNLVVRRGHYTGQLMLVLVTTRPK
                     IFRIDQMIEKLVSAFPSVVSIMQNINDRNSNVIFGKEFRTLYGSDTIEDQMLGNTYAI
                     SAQSFYQVNTEMAEKLYQKAIDFSDLNSEDIVIDAYSGIGTIGLSVAKQVKHVYGVEV
                     VEKAVSDAKENATRNGITNSTYVADSAENAMAKWLKEGIKPTVIMVDPPRKGLTESFV
                     YSAAQTKADKITYISCNSATMARDIKLFEELGYHLVKIQPVDLFPMTHHVECVALLVK
                     A"
     gene            3067..3288
                     /locus_tag="VBC28_001856"
     CDS             3067..3288
                     /locus_tag="VBC28_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6815807.1"
                     /translation="MPTMQLLDELVLPKDITIADVSQSIQEFEIERDALQEFFDDMLK
                     TYTTYQEMKDYIKSREANTPKQETEEKSL"
     gene            3406..4143
                     /gene="aphA"
                     /locus_tag="VBC28_001857"
     CDS             3406..4143
                     /gene="aphA"
                     /locus_tag="VBC28_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003057376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HEO6815808.1"
                     /translation="MKKVLVSSLLVLGITITLQTVVEAKGPKVAYTQEGMTALSDTNK
                     DKVTTISIDEIQKSLEGKKPITVSFDIDDTLLFSSQYFQYGKEYVTPGSFDFLHKQKF
                     WDLVAKRGDQDSIPKEYAKKLIAMHQKRGDKIVFITGRTRGSMYKEGEVDKTAKALAK
                     DFKLDKPIAVNYTGDKPKKPYKYDKSYYIKKYGSDIHYGDSDDDIHAAREAGARPIRI
                     LRAPNSTNLPLPEAGGYGEEVLENSAY"
BASE COUNT         1579 a          655 c          850 g         1381 t
ORIGIN      
        1 tgataccgat tatcttacac cacaaaaaat agctactgta attggagagt cactggttaa
       61 tttatctaac taagttatca cagtttggta gtatgaacta ttattattga aagtaaatca
      121 cctgaaaata tgtcaagaaa taattacggt cagctaaatc atcatgaaga gctacgatat
      181 aactatcttt taaaaaacat ccattatctt aatgaacgtg aaaagatgga atttcagtac
      241 cttcattata aaaaaacagc agtacgacca cagagaagga cagaaagtcc accaactaat
      301 tcttattatg aggaacctta ttctgattct tattatcaag atgacgattt ttactcagag
      361 cctcagctaa cctcacaagg tttaccaatt tatcaagaag agagagctcc gaagaagaaa
      421 aaacaaaggg ccagaaaaga aaaacagagg gtgaaggtta tggcgccttt ccctccaaag
      481 gctataaccc cccctagaaa gaaaaaaaaa tttaagggtt tcctaaagtt cataggtatt
      541 atattactaa ttgttttatc aggtatggtt ttcatgtttg ttaaaggaat gcgtgatgtg
      601 aataatggga agtcacatta tagccctgca attattgaag attttaaggg aaaagatgct
      661 gttgatggga ctaatatctt aattttaggt agtgataaac gtgtcagcga gagatctact
      721 gatgcgagaa cagataccat aatggttgct aatgttggta acaaggataa taaggttaag
      781 atggtgagtt ttatgcgaga tctcttagtc aatattccta attatagtac ggaaggttac
      841 tatgatatga aattaaatgc gtcttttaat ttgggagaac aagataatca taaaggagca
      901 gagtatgttc gacaaacgtt gaaaaatcat tttgatattg atataaaata ttatgttatg
      961 gttgactttg agacatttgc agatgctatt gatacactgt ttcctaatgg tgtcaagatt
     1021 aatgctaaat ttggacttgt cggtggacaa tctgctgatt cagttaaagt tcctgatgat
     1081 ttaaggatga aaaatggcgt tgtacctagc caaaaaatta aggttgggat tcagtacatg
     1141 gatggaagga cccttctaaa ttatgctcgt ttcagaaaag acgatgatgg ggactttggt
     1201 cgaacacaac gtcaacaaca agttatgaga gctattgtct ctcaaataaa agacccacgt
     1261 cgtctcttta caggttctgc tgctatcggt aaagcctatg ctttaacttc aagtaattta
     1321 tcttatagct ttgtcctaac agatggcatt ccaattttat cagatgctaa aaatggtatt
     1381 aagcaaatga caattccaag agaaggagat tgggtagatg attatgacca gtatggtggt
     1441 caagggttaa caattgattt tgctaagtac aaaaagatcc taaagaaaat gggactgcgt
     1501 taacattttt aattatgata taatagagtg atttattgtc actctatttt tatctttagt
     1561 gtataaacta aagacttcaa atgatagata taaaaggaaa attatgttac ataaaaatga
     1621 tattattgaa actgaaatta gcgacattag ccatgaggga atgggtattg ccaaagtaga
     1681 tggctttgtt ttctttgttg aaaatgctct tccaggtgag attattaaga tgcgcgtctt
     1741 gaaattacgt aagcgtatag gatatggaaa agttgaggaa tatttaacga cttctcctca
     1801 ccgaaatgaa ggattagact atacctattt acgtacaggg attgctgatt tgggacatct
     1861 tacttatgaa caacagctgt tatttaaaca aaaacaagta gctgataacc tttataaaat
     1921 tgctcatata tctgatgttt tagtagaacc tactcttggt atgacaatcc ctttggcata
     1981 tcgaaataaa gctcaggtac cagttcgtcg tgttgatgga cagctagaga caggtttttt
     2041 cagaaaaaat tcacatactc tggtatccat tgaagattat ttaattcagg aaaaagagat
     2101 tgatgctctg attaatttta cacgagattt attaagaaaa tttgatgtta aaccatatga
     2161 cgaagagcag caatctggtt taattagaaa tctcgttgtt cgtcgaggac attatacagg
     2221 gcaattaatg ttggttttgg tgacaacacg tcctaaaata ttccgcattg accaaatgat
     2281 tgaaaagttg gtgtctgcct ttccttctgt tgtttcaatt atgcaaaata ttaatgatcg
     2341 taatagcaac gttatttttg ggaaagaatt tcgtactcta tatggttctg atactattga
     2401 ggaccaaatg ttaggaaata cttacgctat atcagcccaa tctttttacc aagttaatac
     2461 tgaaatggct gaaaaactgt accaaaaagc aatcgatttt tcagatttaa actcagagga
     2521 tatcgttatt gatgcctatt caggtatagg aacaattggc ttatctgttg ctaaacaagt
     2581 aaaacatgtt tatggtgttg aagttgttga aaaagcagta agtgatgcca aagaaaatgc
     2641 tactcgaaat gggataacta acagtaccta tgttgctgat tcggctgaaa atgcaatggc
     2701 aaagtggctt aaagaaggta taaaaccaac tgttataatg gttgatccac cacgaaaagg
     2761 attaacggag agttttgtat attctgctgc ccaaacaaaa gcagataaaa tcacttatat
     2821 ttcttgtaat tcagcgacaa tggcgcgtga tatcaaacta tttgaagaat tagggtatca
     2881 tttagtaaaa attcaaccag tagacttatt cccaatgaca catcatgtgg agtgtgtggc
     2941 tctgctcgta aaagcctaga aactttttaa cgaaaggaat attaaaaagc cttagataat
     3001 cgactgacct attaccaaaa aatcgaaggg gctctacttg ccattgcaca aacaccagga
     3061 aatagtttgc caactatgca gctgttagat gagttagttc ttccaaaaga cattaccata
     3121 gcagatgtca gtcaaagtat ccaagaattt gaaatagaaa gagatgccct tcaagaattt
     3181 tttgacgata tgctgaagac ttatacaacc taccaagaaa tgaaagatta tattaaatct
     3241 cgtgaagcga acactcctaa gcaagagaca gaagaaaaat cactttgaag actgtagact
     3301 taacataagt aaaaaataac catatatgat atcgctttca ttcttatgtt ttttgtgata
     3361 aactaattaa agaaataatt tttatacttt attatggagg ttcttatgaa gaaagtgtta
     3421 gtgagtagtc ttttggtttt agggattacg ataacgttac aaacagtagt tgaggctaag
     3481 gggccaaaag tagcttatac acaagaggga atgactgctc tttcggacac aaataaagat
     3541 aaagtcacta ctatttctat tgacgagatt caaaaaagct tagaaggtaa gaagccgatt
     3601 actgttagtt ttgatattga tgatacactg cttttcagta gtcaatattt tcaatatggt
     3661 aaagaatatg taactcctgg atcgtttgat tttcttcata aacaaaaatt ctgggatctt
     3721 gttgcaaaac gaggagatca agattccatt cccaaagaat atgctaaaaa attaattgct
     3781 atgcatcaaa aacgaggaga taaaattgtt tttataacag gtaggacaag agggtcaatg
     3841 tataaggagg gcgaggttga taaaacagct aaagccttag ctaaagattt taaattagac
     3901 aaaccaattg ctgtaaatta tacaggcgat aaacctaaaa agccatacaa atatgataaa
     3961 tcatattata ttaagaaata tggttcagac attcattatg gagatagtga tgacgatatt
     4021 catgcagcta gggaggccgg tgctagacca attagaattt taagagcacc taattctaca
     4081 aatctacctt taccagaagc tggaggctac ggtgaagagg ttctcgaaaa ttcagcttac
     4141 tagtatcttt attagttaac atagtattat ctatcaattt ttagatttgt aataagaagt
     4201 gctaaaaaag tagcaaatca atgatatgct aataagggaa gttggagtag tttagattga
     4261 ttattaatga ctgatcttct cccctctcgg taacaagaag tttttctaac aaatgcctgt
     4321 ctaaaaaaat tgaatcataa ttttttaata actcttttgg cgtgaacaag gaatttgtaa
     4381 tcagtcatct aattctataa tacaatactt tggggttgtt tccgtatcat tattaataat
     4441 atgtatcaga aaggagatgt ttttt
//
LOCUS       DATRMA010000106         4348 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.107, whole genome shotgun sequence.
ACCESSION   DATRMA010000106 DATRMA010000000
VERSION     DATRMA010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4348
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.107"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            complement(<1..408)
                     /locus_tag="VBC28_001858"
     CDS             complement(<1..408)
                     /locus_tag="VBC28_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO6815809.1"
                     /translation="MEKELGKTLRRLRKGKKVSISSLADEHLSKSQISRFERGESEIT
                     CSRLLNILDKLNITIDEFVSIHSKAHTHFFILLNRVRKYCAEKNVTKLVALLEDHNHK
                     DYEKIMIKALIFSIDQSIEPNQEELARLTDYLFT"
     gene            496..564
                     /gene="shp2"
                     /locus_tag="VBC28_001859"
     CDS             496..564
                     /gene="shp2"
                     /locus_tag="VBC28_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_128650799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide pheromone SHP2"
                     /protein_id="HEO6815810.1"
                     /translation="MKKINKALLFTLIMDILIIVGG"
     gene            625..2217
                     /locus_tag="VBC28_001860"
     CDS             625..2217
                     /locus_tag="VBC28_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase"
                     /protein_id="HEO6815811.1"
                     /translation="MKIKKIISGLAAALIISSLSTINYEVKADDTTSEYHYISKQNNE
                     EQLISYIKEQHRLLNQFVVDNVNSFTQLNANPTIEQLNRAITLFKQKDEQLFNQVKAG
                     HLSPSNYNAIVNQRNVINQTVQNLIDQNHNKLQTSQNKAAQLVGQRNQVVNKIQAILA
                     TVNYNSVNSIQEAENLFHSLRNQIEPLVAEVNNYKAAMAILQQEVDALSTAAIETETS
                     KLATLKVSENTSVPANKVEEKTTQSEASGNKQEVTKSEEKQATSDAKASQPESANIAD
                     YDSLKEVLRNNISNQVPHISVQMEFKTQEQVDEYQKNLGSIIREIGDTLGTATEFNAK
                     SNISTYTLGGQIQRIIVKSDITITYTLKGDMVGLHKEYKQFVDSFVKENITNKNITSD
                     YEKAKVIHDHLVNNYTYATEELATTRETASGISIHAPEALYKDKRGVCQAFAVMFKDM
                     AATAGLSVWYVTGQAGGGNHAWNIVTINGVKYYVDTTWDNNIKSNKYFLVGKTIMDAD
                     HLLDSQYNALAKDIPADRHQGA"
     gene            2253..2429
                     /locus_tag="VBC28_001861"
     CDS             2253..2429
                     /locus_tag="VBC28_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6815812.1"
                     /translation="MSKKTDSNYLHSNCSDKKIWSDEAPLQQETIPKDRDCVIKPVIT
                     MTVEHVEIPLDDQS"
     gene            complement(2480..2674)
                     /locus_tag="VBC28_001862"
     CDS             complement(2480..2674)
                     /locus_tag="VBC28_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001878969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6815813.1"
                     /translation="MLFLMIKPDYIRYFKVIDDMHDTMYQQTVFHVQLQAWKYTMKSI
                     DQLVVDDLNHISNEKTTLVK"
     gene            2673..2867
                     /locus_tag="VBC28_001863"
     CDS             2673..2867
                     /locus_tag="VBC28_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6815814.1"
                     /translation="MIMSGQRATSTIQSDINIFHHIEAICISKSNSIGLPAIQNHLGQ
                     LIQRERLGLFFSITYSKAPT"
     gene            complement(3206..3460)
                     /locus_tag="VBC28_001864"
     CDS             complement(3206..3460)
                     /locus_tag="VBC28_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001923489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEO6815815.1"
                     /translation="MFVMRTFGNSLSGPLVVILSSILFSWSHLHGLSVVDFVVYFGMG
                     LIFASLHHYTKSIHYSIGEHIVWNSLSYIFYFLTFLLDLL"
     gene            complement(3479..3730)
                     /locus_tag="VBC28_001865"
     CDS             complement(3479..3730)
                     /locus_tag="VBC28_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000973012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6815816.1"
                     /translation="MMILLSYVLTKTEPISNYQESSSNLKKWLFAIGAFSVLFCWALL
                     VKTFFPIRQNQEGLIADMNQVNHYFRVVLLLTICLIAPI"
BASE COUNT         1597 a          770 c          671 g         1310 t
ORIGIN      
        1 cgtaaaaaga tagtctgtta agcgtgctaa ttcttcttga ttaggctcta ttgattgatc
       61 aattgaaaaa ataagagcct taatcataat cttttcatag tctttatggt tatgatcctc
      121 taagagagcc actaatttag ttacattttt ttcagcacaa tattttctta ctcgatttaa
      181 aaggataaaa aaatgagtgt gcgccttaga atgaatcgac acaaattcat caatagtgat
      241 gttgagtttg tctaaaatat taagcaaacg actacaagta atttctgact ctcctctctc
      301 aaaccttgag atttgtgact tggataggtg ctcatctgct aaagaagaaa tactaacttt
      361 ttttccttta cgtaatcttc ttagtgtttt tcctaattct ttttccattt ttcccacttt
      421 cacaacaatt taaaagaccc tttaaatcca ttatataata caagtacaaa atagaaaatc
      481 agaaaggaga tagtcatgaa aaaaattaac aaagctttgt tgttcacatt aatcatggat
      541 atcctaatta tcgttggtgg ataaaactcc ccacaaatgg ttgttatgga atcccatttt
      601 atttagttta taacaggaga actaatgaag attaaaaaaa ttattagtgg ccttgccgca
      661 gctttaatta tcagttcact atcaactatt aactatgagg ttaaagctga tgacaccacc
      721 agtgagtatc actatatcag taagcaaaat aatgaagagc agcttattag ttacatcaag
      781 gaacaacatc gtttgctcaa tcaatttgtt gttgataatg tcaactcatt cactcaacta
      841 aatgctaatc caactattga acagttaaat agagctataa cattatttaa acaaaaagat
      901 gagcaattat ttaaccaggt gaaagctggt catctctctc ccagtaacta taatgctatc
      961 gttaatcaac gtaatgtcat taaccaaact gttcaaaatc tgattgacca aaatcataat
     1021 aagcttcaga caagtcaaaa taaagcagct cagctcgtgg ggcaacgtaa tcaggttgtt
     1081 aacaaaattc aagctatttt agcaactgta aactacaact ctgtgaattc tatacaagaa
     1141 gctgaaaatt tatttcattc actcagaaat caaattgaac ctcttgtagc tgaagttaat
     1201 aattacaaag ctgctatggc aatccttcaa caagaagtag atgccctatc aacagcggct
     1261 attgaaactg agacttctaa acttgctact ctcaaagtta gcgaaaatac ttctgttcct
     1321 gcaaacaaag tagaagaaaa aactactcaa tcagaagcgt caggcaataa acaagaagta
     1381 actaagagtg aggaaaaaca ggctacctct gatgcaaagg catcacagcc tgagtcagct
     1441 aatattgccg attacgatag tttaaaagaa gttttacgaa ataatattag caaccaagta
     1501 ccacacatca gtgttcaaat ggagtttaaa actcaagaac aagttgacga ataccaaaaa
     1561 aatctcggaa gcatcatccg ggaaattgga gatacacttg gaacagcaac tgaattcaat
     1621 gccaaaagta acattagcac ttatactctt ggtggacaaa tccaacgcat tattgtaaaa
     1681 agcgacatca caatcaccta tactcttaaa ggtgacatgg taggattaca taaagaatat
     1741 aaacagtttg tagattcttt tgtcaaagaa aatattacta acaaaaatat cacaagtgat
     1801 tatgaaaaag ctaaagtaat tcatgaccac ttggttaata attacactta cgcgactgaa
     1861 gaactggcaa ccactcgtga aactgctagt ggtatcagta tccatgctcc tgaagcactc
     1921 tacaaagata aacgtggtgt ttgtcaagcc tttgcagtaa tgtttaaaga tatggctgct
     1981 actgctggct tatcagtatg gtatgtaact ggtcaagctg gaggtggaaa tcacgcttgg
     2041 aacattgtta ctattaatgg cgttaaatat tatgttgata caacatggga taataatata
     2101 aaaagcaata aatatttcct tgttggtaaa acaataatgg atgctgatca tcttttggat
     2161 agtcaataca atgcattagc taaagatatt ccagctgatc gccatcaagg tgcataactc
     2221 aaaaattgta ttgagcctgg aaaggatatg aaatgtctaa aaagactgat tcaaattatc
     2281 tacattccaa ttgttctgac aagaaaattt ggagcgatga ggcgcctctg caacaggaga
     2341 ctatccctaa agacagagat tgtgttatca aacctgtcat tactatgaca gttgaacatg
     2401 tggaaatccc attagatgac caatcttagc tctctcctaa aataaaaagg aacactgcca
     2461 tttttgacag tgttcctttt tatttaacca aagtggtttt ctcattactg atatgattca
     2521 aatcatctac aactaattga tcaatcgatt tcatggtgta tttccaagct tgcagttgaa
     2581 catgaaaaac agtctgttgg tacatggtat catgcatatc gtcaatgact ttgaaatacc
     2641 ttatataatc tggtttaatc attaggaaca gcatgatcat gagtggtcaa agagctacca
     2701 gcaccatcca gagcgatata aatatctttc atcatataga agctatttgt ataagtaaat
     2761 ctaacagtat tgggctcccc gcaatacaaa atcacttggg acagctgatc caaagagaac
     2821 gtctgggtct tttcttctcg ataacctatt ccaaagctcc cacttgaagt acttactgaa
     2881 tctgttgcaa cagaaaaaca atccctacta ctagaataat agcacctcta actaataaat
     2941 gactcattcc aacattaaca tcttttaaca tcatgttcct ttcattattt tttatttagg
     3001 aattcgtcat actatatttt ctcatattta tctaacaaaa aactcgtaat tctatataaa
     3061 tcatataaag ttacgaactt tatttagtta acaataaaat aataacattt tattcattaa
     3121 aaaaacgaat aacaaaagag ccaaaaagct cttttaaact gacaaaaatg tcttagtaat
     3181 gtgattagtc tctcaatctt attacttaga gtaaatcaag caaaaatgtt agaaaataaa
     3241 aaatatatga caaactattc caaacaatat gctcgccaat cgaataatga atggattttg
     3301 tatagtggtg caaactagcg aaaatcaaac ccataccaaa atagacaaca aagtcgacga
     3361 cagatagacc atgtaagtga gaccagctaa atagtataga agataagatc acaaccaagg
     3421 gaccactcag ggagttccca aatgttctca tgacaaacaa cctgaacgtt atctcttctt
     3481 aaattggggc aataagacag atagtcaata ataagacaac tcgaaagtag tgattgacct
     3541 gattcatatc agctattaat ccctcctggt tttgccttat tgggaaaaat gtttttacta
     3601 gtagtgccca gcaaaataaa acgctaaaag cacctattgc aaatagccat tttttgaggt
     3661 tagatgatga ctcttggtag ttactgattg gctcagtttt tgttagcaca taactcaaca
     3721 gaatcatcat tataagatta atgccaatcc atatactcca actccactct aaccctattg
     3781 atagtaaact aatcacccaa tgcccaagca attgttatta ttgtgttgtt gcaactttgg
     3841 caaagatgaa tatagcagga tggaaaacag attgtttctg aaacttcaaa ggtatcttct
     3901 tatcacaagc tattatttgg ttatctccat aattaatcta aatggagcaa tatatcgttt
     3961 ttctactaac tgttgacaaa taaaaaactc ccctttaaag tctggtaact tttcgatttt
     4021 taccaaaaac ttaaggagag tgaggcaaga actaatagaa tcttatttaa agttttatga
     4081 ttgggagaac agaacaacta tcataattac tgaaaaatta tgaaaggcat ggcaaacaac
     4141 agatggtcaa atccgctctg ttgaaaacaa attttaaata taattcattt cactttctaa
     4201 gtttattaaa catagagtgt tcaggagaaa tgatacgacc taatgataaa atcattccta
     4261 tcgtataata aacaacgaca aggtgtaaat aaaagtaaat taatatatct gatatacttg
     4321 tcaaacatcc tttcattata ataaaagc
//
LOCUS       DATRMA010000107         4311 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.108, whole genome shotgun sequence.
ACCESSION   DATRMA010000107 DATRMA010000000
VERSION     DATRMA010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4311
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.108"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            18..1757
                     /locus_tag="VBC28_001866"
     CDS             18..1757
                     /locus_tag="VBC28_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEO6815817.1"
                     /translation="MTLFLIMMERAGLIILLAYAFVHIPFIKQTLKQPELKKHQYILL
                     ILFSLFAIISNFTGVEIQSDLSIIPQTLNHIADQSSVANTRVLTIGVSGLIGGPIVGI
                     IVGLLSVFVRYLQGGLAPHIYVISSLLIGLCSGLSGNYLRKNYNKIRVLDAMVVGFGM
                     EILQMICILIFSVDFNQALRLVSFISMPMILSNTLGLGIFISIISSTQKLEEHAKAFQ
                     THQVLELANLTLPYLRKGLTTESCQPVAEIIHKHMDVSAVSLTSQSAILAYVGDGADH
                     HLPNTQILTKLAKRAIDTGKVSVATDKSEIECDHKNCPLSSAIVIPLHIHDVIVGTLK
                     LYFSDAQHMTYVDRQLAEGLGNIFSTQLALGQAEEATRLLQDAEMKSLQAQVNPHFLF
                     NALNTIYGLIRMDSEKARKLVQDFSKVIRANLQRAKQNLIPLHDELEQVNAYLALEEA
                     RFPNMVAFNLDNQTNSDDNLMIPPFTLQVLIENSYKHAFKHVNKNNQLKVTIARNNDR
                     LHIIVQDNGIGIPKEKLITLGKKTQISKQGSGTAIENLVRRLNIIYDGQASLKFESND
                     SGTCAIVNIPIKN"
     gene            1769..2503
                     /gene="ltdR"
                     /locus_tag="VBC28_001867"
     CDS             1769..2503
                     /gene="ltdR"
                     /locus_tag="VBC28_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000866963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor LtdR"
                     /protein_id="HEO6815818.1"
                     /translation="MKVLVVDDEPVARNELIYLLNKYDSNLVIAEAHDMATALAILLR
                     ETFDVALLDIHLRDDSGLQLAEYINKMPKPPLLIFATAYDQYAIQAFEHDARDYLLKP
                     YDFDRLKQAMDRVKGALSTSTIIESVTSGPLFKQQYPLTVEDRIYLVSADDILLIEAM
                     QGKLIIQTPDKNYEIDGSLQQWQDKLPSSQFVRVHRSYIVNINAIKTIEPWFNQTLQL
                     HLCNKITVPVSRANVKPLKQMLGIST"
     gene            2659..4143
                     /locus_tag="VBC28_001868"
     CDS             2659..4143
                     /locus_tag="VBC28_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HEO6815819.1"
                     /translation="MVTFLGGVALLIVGYFTYGRYIEKNFQIDENRQTPAEALRDGYD
                     FVPMPKWKNGMIELLNIAGTGPIFGPILGALYGPVAYIWIVLGCIFAGAVHDYMIGMI
                     SLRNNGAYLPELASRYLGKSMKHVINIFSMLLLILVATVFVVTPANLILSILPAGTLS
                     LPWIIGLIFVYYLISTVLPIDKALGKVYPVFCVILMVSTAAVGFRLLTGGFDMPNLTF
                     ETFKNMHPAGLGIFPALFFTISCGAISGFHATQAPMVSRTTVNEREGRFTFYGMMIAE
                     GVIAMIWAGASMSLFKGQNLYEMIAAGTPSAVVNQVMLMLLGSVIGTIAIIGVIVLPV
                     SSGLSAFRSLRTIVADYIHVKQDTLPKIFAVTIPLYVISFVLTHVDFNLLWRYFNWAN
                     QVTAVIGLLVATRYLILKRRNYWVTFVPAMFMLYAVVVYILSQPIGFNMGLGILTYSL
                     ALVLTGILVGLFWKSGQKQLKTVHPEAFLFNDHRPINYYSSLDS"
BASE COUNT         1345 a          746 c          753 g         1467 t
ORIGIN      
        1 tactttggag agatttaatg acactctttt taattatgat ggagcgagcg gggttaatca
       61 tattattagc ctatgccttt gtgcatatcc cctttataaa acaaactttg aagcaacccg
      121 aattaaaaaa acaccagtat attctgctta tcttatttag cttatttgct attatttcta
      181 attttacagg ggtagaaatt cagtcagatt tatctatcat tccacaaact cttaatcata
      241 tagcagatca atcatcggtt gccaatacac gtgttttaac cattggtgta tcaggtctaa
      301 tcggtggccc catagtcggt attatagttg gtctcttatc tgtattcgtt aggtatttgc
      361 aaggaggatt agcccctcat atttatgtca tttcttcctt actaattggt ttatgctctg
      421 gattatcagg caattatctt cgtaaaaatt ataataaaat tcgtgtttta gatgcaatgg
      481 ttgttggttt cggtatggaa attctacaaa tgatctgtat cctcattttt agtgttgact
      541 ttaatcaggc gcttcgctta gtatctttta tcagcatgcc aatgatttta agtaatactt
      601 taggtttagg tatatttata tccattattt catctactca aaaattagaa gaacatgcta
      661 aagcatttca aacccatcaa gtacttgaat tagcaaattt aacccttcct taccttagaa
      721 agggactgac aacagaatct tgtcaaccag tagctgaaat tattcataaa catatggatg
      781 tgagtgctgt tagcctgact agtcaatcag ccattttagc ctatgtagga gatggagctg
      841 accatcatct tcctaatact caaatattaa caaagcttgc taaaagagca atagatactg
      901 gtaaagttag tgtcgcaact gataaaagtg aaatcgaatg cgaccataag aattgcccac
      961 ttagttcagc aattgttatt ccattacaca tccacgatgt tatagtgggt actttgaaat
     1021 tatatttttc agacgcacaa catatgactt acgttgaccg tcaattagct gaaggattag
     1081 gcaatatctt ttccactcag ttagctttgg gacaagctga ggaagcaaca aggttactac
     1141 aggatgcgga aatgaagtct ctccaagcgc aggttaatcc gcattttctt tttaatgcac
     1201 ttaatactat ttatggttta attcgcatgg atagcgaaaa agcaagaaag ctcgtccaag
     1261 attttagcaa ggttattcgt gctaatcttc aacgggcaaa gcaaaattta attcctcttc
     1321 atgatgaatt ggaacaggtt aacgcctatt tagcccttga agaagcaaga tttcccaaca
     1381 tggtggcctt taatttagac aaccaaacaa attcagatga caacttaatg attcctccct
     1441 ttacattgca ggtcttgatt gaaaactctt ataaacacgc ttttaagcat gttaataaaa
     1501 ataaccagct aaaggttact attgcaagaa ataatgatag gcttcatatc attgtccaag
     1561 acaatggtat tgggattcca aaagaaaaat taataacgct tggaaaaaaa actcaaattt
     1621 caaaacaagg ttctggtaca gctattgaga atctcgttcg tcgcttgaat attatttatg
     1681 atggacaagc aagtttaaaa tttgaatcaa atgattcagg aacttgtgct attgttaata
     1741 tccccattaa aaattagaaa ggtattgtat gaaagtttta gtagttgatg atgaaccagt
     1801 tgcacgtaac gaattaattt accttcttaa taagtatgat tctaacctcg ttatagcaga
     1861 ggcgcatgat atggctactg cattagctat tttacttaga gaaacttttg atgtagcact
     1921 gttagatatc catctcagag atgattctgg gttgcaatta gcagagtata tcaataaaat
     1981 gcccaaacca ccattattga tatttgcgac tgcttatgat caatatgcta ttcaggcttt
     2041 tgagcatgat gcgcgtgatt atttgttaaa accctatgat tttgataggc taaagcaagc
     2101 tatggataga gtaaaaggag cgctaagtac atctacaatt atagagagcg taacttccgg
     2161 tcctctcttc aagcaacagt atccattgac agtagaagat cgaatctatc tggtgtcggc
     2221 ggatgatatc cttttgattg aagctatgca aggaaaactg attatacaaa cacctgataa
     2281 aaattatgaa attgatggct ctctacaaca atggcaagat aaactaccat catctcaatt
     2341 tgtacgggta catcgctctt acattgtgaa cattaatgct attaaaacga ttgaaccttg
     2401 gtttaaccaa acacttcagt tacacctttg taataaaata acagttcctg ttagcagagc
     2461 aaatgtaaaa cccctaaaac aaatgttagg catatctacc taactaatga aaagttattc
     2521 atcataaaac ttagactatt catttctaaa aaacaaccat tcatcttgaa aataggaaat
     2581 ttcaattttt caatgctata ctaaagtcat gaaaacgctt aataaaatta agcaaaaaaa
     2641 tttttaggag gcagtgatat ggtaacattt ttaggagggg tagccctttt aattgttggt
     2701 tatttcacgt acggtagata cattgaaaag aattttcaaa ttgatgaaaa tcgccaaaca
     2761 cctgcagaag cattaaggga tggatatgat tttgttccta tgccaaaatg gaagaatggg
     2821 atgattgagt tattgaacat cgcaggaacc ggacctatct ttggacctat tttaggagca
     2881 ctctatggtc cagttgctta tatctggata gtcttaggat gtatttttgc aggggctgtt
     2941 catgattata tgattggaat gatttctctt agaaataatg gtgcatatct tcctgaatta
     3001 gctagtcgct atcttggaaa gtcaatgaag catgtgatca acattttttc aatgctatta
     3061 cttattttag ttgcaaccgt atttgtggta actccagcta atcttatcct aagtatttta
     3121 ccagctggga cactatcttt accttggata atcggtctta ttttcgttta ttaccttatt
     3181 tctacagtct tacctattga taaagcactt ggtaaagttt atcctgtatt ctgcgtcatc
     3241 ttaatggtta gtacggcagc agttggcttc cgtttattaa ctggtggctt tgatatgcca
     3301 aacttaactt ttgaaacctt taaaaatatg catccagctg ggcttgggat attccctgct
     3361 ttattcttta ccatttcatg tggagccatc tctggtttcc atgctaccca agcacctatg
     3421 gtctcaagaa caactgttaa tgagagagaa gggcgtttca ctttctacgg catgatgatt
     3481 gcagaaggag taattgcgat gatttgggca ggtgcttcaa tgagcttgtt caaaggtcaa
     3541 aatctctatg aaatgattgc tgctgggaca ccttctgcag ttgtcaatca agtaatgtta
     3601 atgttactcg gtagtgtaat tggaactatc gctatcattg gtgttattgt tctccctgtt
     3661 tcatctggtt tatcagcttt ccgtagctta cgtactatcg ttgctgatta tatccatgtc
     3721 aaacaagata ctcttccaaa aatctttgct gtaactatcc cattatatgt tatttcattt
     3781 gtccttacac acgttgattt taatctccta tggcgttact ttaactgggc aaatcaagta
     3841 actgctgtta ttggtttatt agttgctaca agatatctca tcctaaaaag acgaaattat
     3901 tgggtaacat ttgtaccagc tatgttcatg ttgtatgcag tagttgttta tattttatct
     3961 caaccaattg gatttaatat gggcttaggc atcttaactt attcacttgc tttagtgtta
     4021 acaggtattc ttgttggact tttctggaaa tctggtcaaa aacaattaaa aactgttcat
     4081 cctgaagcat tcctgtttaa cgatcataga ccaattaatt attacagtag tttagattct
     4141 taatattatt ttgaagccat gttattaaga cttacagtta tcacaataac ctcgaatgaa
     4201 gtatcattcg aggttatttt tctactaaaa attccagtaa atatttctgt ttgattacaa
     4261 atgttacaaa aatatttgac aaccttcttt cagaaactta taatagattt g
//
LOCUS       DATRMA010000108         4134 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.109, whole genome shotgun sequence.
ACCESSION   DATRMA010000108 DATRMA010000000
VERSION     DATRMA010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4134
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.109"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            60..1085
                     /gene="neuB"
                     /locus_tag="VBC28_001869"
     CDS             60..1085
                     /gene="neuB"
                     /locus_tag="VBC28_001869"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HEO6815820.1"
                     /translation="MVYIIAEIGCNHNGDINLAKKMVDVAVSCGVDAVKFQTFKAEKL
                     ISKFAPKAEYQKATTGTADSQLEMTKRLELSFEEYLEMRDYAISKGVETFSTPFDEES
                     LEFLISTDMPIYKIPSGEITNLPYLEKIGKQQKKVILSTGMAVMEEIHQAVNILRQNG
                     TTDISILHCTTEYPTPYPSLNLNVIHTLKDEFKDLTIGYSDHSIGSEVPIAAAAMGAE
                     VIEKHFTLDTNMEGPDHKASATPDILAALVKGVRIVEQALGRFEKIPDPVEEKNKIVA
                     RKSVVALKPIKKGDIYSIENITVKRPGNGISPMNWYDILGQEAQDDFEEDEVIRDSRF
                     ENQLPEL"
     gene            1162..2316
                     /gene="neuC"
                     /locus_tag="VBC28_001870"
     CDS             1162..2316
                     /gene="neuC"
                     /locus_tag="VBC28_001870"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HEO6815821.1"
                     /translation="MKKICLVTGSRAEYGIMKPLIQRLSKDKEVNLQIIATAMHLEEK
                     YGYTYRQIEEDGFDIAYKVPLHLYDTDRRTVSTAMAHLQLGLTKIFDKEDYDLVIILG
                     DRYEMLPVVNVALIYNVPVCHLHGGETSLGNFDEYIRHAITKMSHLHLVSTEDFRQRV
                     IQMGEQPQFVINTGALGVENALSIPSLTKEAIEKQLGIVLEESYFVVLYHPVTFEQGK
                     SAGEQMKAVLSALSKFGVQCLFIGSNSDTGSDDIAKAINTYLINHENSYCFASLSTQL
                     YHSLIRHSLGLIGNSSSGLIEVPSLMKPTLNIGDRQKGRLHGESVVSVPVETSSVLEG
                     LSKLNEVTNFDNPYYKGNASSIAYEAIKLYLKDEPSISQPFYDLKENNLK"
     gene            2313..2942
                     /locus_tag="VBC28_001871"
     CDS             2313..2942
                     /locus_tag="VBC28_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEO6815822.1"
                     /translation="MKKKLAFLGAGSHADAIAPVIDPLVYDFVGFFDDKDITEHDGYP
                     VLGKLYDVLPYLEDGSIDAVFITIGDNAKRKELFEYVAKDYYDFIINIISPNALVLTP
                     DSICGRGIFIGFGAFIGSKVKLFDNNVVNTGALIEHHTVVESHCNIAPNATINGLCYI
                     REEVYVGSASVIIQTLDISSCTTIGAGAVVVKDIIEPGTYVGVPAKKIK"
     gene            2953..>4134
                     /gene="neuA"
                     /locus_tag="VBC28_001872"
     CDS             2953..>4134
                     /gene="neuA"
                     /locus_tag="VBC28_001872"
                     /EC_number="2.7.7.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000802346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acylneuraminate cytidylyltransferase"
                     /protein_id="HEO6815823.1"
                     /translation="MKPICIIPARSGSKGLPDKNMLFLAGKPMIFHTIDAAIESGMFD
                     KKDIFVSTDSELYREICLERGISVVMRKPELSTDQATSYDMLKDFLSDYEDNQEFVLL
                     QVTSPLRKSWHIKEAMEYYSSHDVDNVVSFSEVEKHPGLFTTLSDKGYAIDMVGADKG
                     YRRQDLQPLYYPNGAIFISNKETYLREKSFFTSRTYAYQMAKEFSLDVDTRDDFIHVI
                     GHLFFDYAIREKENKVFYKEGYSRLFNREASKIILGDSKTISISLENYHNYSQGGVTL
                     ATMLENLPNFLTANVTEAFVSIGVNDLITGYSVEEIFSNFQKLYSLLAENKIKMRLTT
                     IAYTLFRETVNNADIEKINQWLTEFCYQNQIPLLDINRFLSKDGNLNYHLTSDGLHFT
                     QE"
BASE COUNT         1390 a          632 c          800 g         1312 t
ORIGIN      
        1 agcattgttt ttcaaaaagt cattttagac ttattgagta aaaaaagaag gagaagataa
       61 tggtttatat tattgcagag attggttgca atcataatgg agatattaat cttgcgaaaa
      121 aaatggtaga tgttgccgtg tcttgtggtg ttgatgctgt taaatttcag acttttaaag
      181 ctgagaaact tatttctaaa tttgctccca aagctgaata tcaaaaagca actacaggaa
      241 cagcagacag tcaacttgag atgacgaaac gtttagagtt aagctttgaa gaatacttag
      301 aaatgcgtga ttatgcaatt tcaaaaggtg tggagacctt ttcaacacct tttgatgaag
      361 agtcattaga gttcttaatt tctacagata tgccaattta caaaattcca tcaggagaaa
      421 tcactaattt accttactta gaaaagattg gcaagcaaca aaagaaagtt attctttcga
      481 cgggtatggc ggtaatggaa gagatccatc aagcggtgaa tattttacgt cagaatggta
      541 caaccgacat ttctatttta cattgtacaa cagagtaccc aacaccttac ccctctctaa
      601 atttaaacgt tattcatact ttgaaagatg aatttaaaga tttaacgata ggttattcgg
      661 atcattcaat tggatcagaa gtacctatcg cagcagcagc aatgggtgca gaagttattg
      721 aaaaacactt tactttagat actaatatgg aaggtccgga tcataaagcc agtgcaacac
      781 ctgatatttt agctgcttta gttaaagggg ttcgcattgt tgaacaagcc ttaggtagat
      841 ttgaaaaaat cccagatcca gtagaagaga aaaataagat tgttgctcgt aaatcagtcg
      901 ttgctttaaa accaattaaa aaaggcgata tttattcaat agaaaatatt acggtgaagc
      961 gcccaggtaa tggtatttct cctatgaact ggtatgacat cttgggacaa gaagcgcaag
     1021 atgatttcga agaggatgaa gttattcgtg attcacgctt tgaaaatcaa ttgcccgagt
     1081 tataataaca cctaattata atcggcaaca gatgtattac acggctaaga ttatgtaatt
     1141 actatatata gaagggatac gatgaaaaaa atttgtttag taaccggatc gcgtgctgaa
     1201 tacggaataa tgaagccatt gattcaaaga ttatcaaaag ataaagaagt caacttacaa
     1261 attattgcaa cagcaatgca tctggaagaa aaatacggct atacttatcg tcaaattgaa
     1321 gaagacggtt ttgatattgc ttataaagtt cccttacatc tttatgatac tgacagaaga
     1381 actgtatcta ctgcaatggc gcatttacaa ctaggattga ccaaaatttt tgacaaggaa
     1441 gactatgatc tagtcatcat tttaggggat cgttacgaaa tgttaccagt tgtgaatgta
     1501 gcgttgattt ataatgtccc agtatgccac cttcatggag gggagacctc attaggcaat
     1561 tttgatgagt atattcgcca tgcaattact aagatgagtc acctacactt agtctctaca
     1621 gaggactttc gtcaacgtgt gattcagatg ggagaacaac ctcaatttgt aattaacaca
     1681 ggagctctcg gagtggaaaa tgctctatca atcccttctc taaccaaaga agcaatagaa
     1741 aagcaattag gtattgtact agaggaatcc tattttgttg ttctctatca tcctgttact
     1801 tttgaacaag gaaaaagtgc tggagaacag atgaaagcgg tactatcagc cctttctaaa
     1861 tttggtgtcc agtgtctatt tataggatcc aattcagata ctggttctga tgatattgca
     1921 aaggctatca atacttattt gataaatcat gaaaatagtt attgctttgc ttctttatca
     1981 acccaacttt atcattcctt aattcgtcat tcactaggac tgattggtaa ttcgtcttct
     2041 ggtttaatag aagttccttc tttgatgaaa cctactctta atattggtga tcgccaaaaa
     2101 ggtaggctac atggagaaag tgtagtaagt gtaccagtcg aaacctcctc tgtattggag
     2161 ggattatcaa aattgaatga agtgaccaac tttgacaatc cctactacaa agggaatgct
     2221 tcatctattg catatgaagc aataaagctt tatttgaaag atgaaccttc aatttcccaa
     2281 ccattttatg atttaaagga gaataatcta aaatgaaaaa aaagttggca tttttaggcg
     2341 caggaagtca tgctgatgcg attgctccgg ttattgatcc tcttgtgtat gatttcgtag
     2401 gtttttttga tgataaagat attacggagc atgatggtta tcctgttctt ggaaaacttt
     2461 atgatgtgct accttacctt gaagatggct caatagatgc agtatttatt acaataggtg
     2521 ataatgctaa aaggaaagaa ctatttgaat atgtagcaaa ggattattat gactttatta
     2581 ttaacatcat tagtcccaat gctttagtat tgacaccaga tagtatttgt gggcgtggta
     2641 tctttattgg ttttggggct tttataggtt ctaaagtgaa gctgtttgat aacaacgttg
     2701 ttaatacagg agcgctcatt gaacatcata ctgttgtaga atcacactgt aatatagcac
     2761 ctaatgctac cataaatggt ctttgttata ttagagaaga agtttatgta ggtagtgcca
     2821 gtgttattat tcaaaccttg gatatttcat cgtgtacgac aattggcgca ggagctgttg
     2881 ttgtaaaaga tattatagaa cctggaacct atgtaggggt tccagccaaa aaaataaaat
     2941 aaaggatatg ttatgaagcc aatttgtatt attcctgcgc gatcagggtc aaaaggatta
     3001 ccagataaga atatgttatt cctagcaggt aaacccatga ttttccatac tatagatgct
     3061 gcaattgaat ctggaatgtt tgataagaaa gatatttttg ttagtacaga ttcagaattg
     3121 tacagagaga tttgtttaga acgcggtatt tcagtggtga tgagaaaacc ggaactttca
     3181 actgatcagg caacttcgta tgatatgtta aaagattttt tatctgacta tgaagataat
     3241 caggagtttg tgttacttca agtaacctct ccactaagaa aatcatggca tataaaggaa
     3301 gcaatggagt attattcttc acatgatgtt gacaatgttg taagtttttc tgaagttgag
     3361 aaacaccctg gtctgtttac gacattgtct gataaaggct atgctataga tatggtggga
     3421 gcagataaag gttatcgtcg ccaagattta caacctttat actatccgaa cggtgctatt
     3481 tttatttcta ataaagaaac ttacttaagg gaaaaaagct ttttcacctc taggacatat
     3541 gcttatcaaa tggcaaagga attttcatta gatgttgata cgagagatga ttttatccac
     3601 gtcatcggtc acctcttttt cgattatgcc attagggaaa aagagaataa agttttttat
     3661 aaagaaggct atagtcgttt gttcaataga gaagcttcaa agataatttt aggtgattca
     3721 aaaacgatat ctatctcact agaaaattac cataattatt cccaaggtgg tgtaacatta
     3781 gcaacgatgt tggaaaactt acctaacttt ttgacagcta atgtaacaga ggcttttgtt
     3841 tctatagggg taaatgatct cattacaggt tatagtgtgg aagaaatatt cagcaatttc
     3901 caaaaactct actcattatt agctgagaat aaaataaaga tgagattgac aactattgct
     3961 tacacccttt ttagagaaac tgtcaataat gcagatattg agaaaatcaa tcaatggcta
     4021 acagaatttt gttatcaaaa tcagattcca ctgttagata ttaatagatt tttatcaaag
     4081 gatggtaatc taaattatca tttaactagt gatggattgc attttactca agag
//
LOCUS       DATRMA010000109         3777 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.110, whole genome shotgun sequence.
ACCESSION   DATRMA010000109 DATRMA010000000
VERSION     DATRMA010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3777
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.110"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            <1..1188
                     /locus_tag="VBC28_001873"
     CDS             <1..1188
                     /locus_tag="VBC28_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011074747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEO6815824.1"
                     /translation="SASTSASTSASTSASTSASTSASTSASTSASTSSSTSASTSAST
                     SASTSASMSASTSASTSASMSASTSASTSASTSASMSASTSSSTSASMSASTSASMSA
                     STSASTSASTSASTSASTSASMSASTSASTSASTSASMSASTSASMSASTSASTSSST
                     SASTSASTSTSTSASTSASTSASTSASMSASTSESISASTSASMSASTSASTSASTSA
                     STSASMSASTSASTSASTSASTSASMSASTSASTSASTSASMSASTSASTSASMSAST
                     SASTSASMSASTSASTSASMSASTSASTSASMSASTSASMSATTSASTSVSTSASTSA
                     STSASTSSSSSVTSNSSKEKVYSALPSTGDQDYSVTATALGLGLMTGATLLGRKKSKK
                     DKD"
     gene            1519..2526
                     /locus_tag="VBC28_001874"
     CDS             1519..2526
                     /locus_tag="VBC28_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001263779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEO6815825.1"
                     /translation="MRVHITSIYGQSPRSIALISQKLVKDVGRQLGYDEMGIYFYNDH
                     AETHGERSTRMDGIIAGLGRGDIVVFQVPTWNSTEFDELFLDKLQAYGARIITFVHDI
                     VPLMFESNFYLLDRVIDMYNRSDVVILPTKAMHDYLIEKGMTTSKVLYQEVWDHPVNI
                     DLPRPECQKVLSFAGDIQRFPFVNDWKENIPLIYYGDGSRLNSEANVHAQGWKDDVEL
                     MLSLSKRGGFGLCWSEDREELVERRYSRMNASYKLSTFLAAGLPIIANHDISSRDFIK
                     QHGLGFTVETLEEAVEKINNMEKETYDSYVENVEKIATLLRNGYITKKLLIDAVHMLY
                     R"
     gene            2535..3740
                     /locus_tag="VBC28_001875"
     CDS             2535..3740
                     /locus_tag="VBC28_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEO6815826.1"
                     /translation="MKKTIVLGADFQYRDQVMTTIKSIVSHNQHLTIYIINTDFPVEW
                     FNILNHSLEQFDCRVKNIPISSDVFEGIPTLSHISVAGFFRWFIPIHLEEEIVLYLDS
                     DVIVRGSLDPLFDINLEENLLGAVADHFSTLYYGDTAPVSFNSGVMLINNSLWKKEEI
                     YNSLMRIADKGSAVGVGDQEYLNILTQNRWIDIGKQYNVQIGQDVNINAYGRPDLYHF
                     YDDCEPVIVHYNSQDKPWNKYSQSRYRSEWWYYFGLEWSVIYAQQQKNLNRLTGKTLN
                     LFTLTASDTLEHIEVLAKALPDYQFHIAARSDISEKLANLETYSNIKVYKLVINPILE
                     ELIRNCDVYLDINHSYEVEGILDKIKEENKPILAFDSTAHTTKNIEVISETMPELMVK
                     RIREVGPLR"
BASE COUNT         1218 a          705 c          772 g         1082 t
ORIGIN      
        1 agtgcatcga catcagcaag taccagcgct tcaacaagtg caagtacgag tgcttccaca
       61 tcagcaagta ctagcgcctc aacatcggca agtacaagtt cttccacaag tgcaagtacc
      121 agtgcatcga catcagccag cactagcgcc tcaatgagtg cctcgacgtc agccagcacc
      181 agtgcttcaa tgagtgcctc gacgtcagcc agcactagcg cctcaacgag tgcgtcaatg
      241 tcagccagca caagttcttc aacaagtgcc tcgatgtcag ccagcactag cgcttcaatg
      301 agtgcctcga cgtcagctag cactagcgcc tcaaccagtg ccagtaccag tgcttccacc
      361 tcagcaagta tgagcgcctc aacaagtgca agtactagcg cttcaacaag tgcaagtatg
      421 agcgcctcaa ccagtgcaag tatgagcgcc tcaaccagtg caagtactag ttcttcaaca
      481 agtgccagta ccagtgcctc gacgtcaaca agtactagcg catccacgtc agcaagtacc
      541 agtgcatcca cgtcagcaag tatgagcgcc tccacaagtg aaagtatcag tgcatccacg
      601 tcagcaagta tgagcgcttc cacaagtgcc agtaccagtg catccacgtc agcaagtact
      661 agcgcctcaa tgagtgcctc gacgtcagcc agcaccagtg cttccacaag tgcaagtact
      721 agcgcctcaa tgagtgcctc gacgtcagcc agcaccagcg cttcaacgag tgcgtcaatg
      781 tcagccagca ccagtgcttc aacaagtgcc tcgatgtcag ccagcactag cgcttcaacg
      841 agtgcgtcaa tgtcagccag cacaagtgct tcaacaagtg cctcgatgtc agccagcaca
      901 agtgcttcaa caagtgcctc gatgtcagcc agcactagcg cttcaatgag tgccacgacg
      961 tcagccagca ccagcgtctc aacgagtgca tcgacatcag caagtaccag cgcttccaca
     1021 agttcttcaa gctcagtgac ttctaattca tcaaaagaga aggtgtattc tgccttacct
     1081 tctacgggtg accaagatta ttctgtaact gctactgcct taggtttagg tttaatgact
     1141 ggtgcaaccc ttttgggacg aaaaaaatct aaaaaagata aagactaaac ctactttgaa
     1201 ttcttaaagt actaaatgac atggtaaaag gcatatctct taatattttt agagagatat
     1261 gcctttttgt tgtagaagtt tgtaaaaagc cttgtaaatc tatatttatc aagtgacaag
     1321 caatgttttt ctgctaaaat atgataaaat taagaagtgg aatttatttc tgaaaataag
     1381 tttatcaatt acgataaact caaaaataat gtaaaaaagc tttggatcat tttagtacaa
     1441 tgagtgttaa taaaactatt atttaactgg tatgatatgg tctggataag agagagtttt
     1501 aaagagagat taactaaaat gcgagtacat attacaagta tatatgggca gtcaccgcgt
     1561 agcattgccc ttatatcaca aaaactagtt aaggatgtcg gacgtcaact tggttatgat
     1621 gaaatgggaa tctactttta taatgatcat gctgaaacgc atggtgaaag aagtactagg
     1681 atggatggta tcattgcggg attaggcaga ggggatattg ttgtttttca agtacctaca
     1741 tggaattcaa cagaatttga tgaacttttt ctagataaat tacaggcata cggtgctaga
     1801 attataactt ttgtacatga tattgttccc ttaatgtttg agtctaattt ttatttatta
     1861 gatagagtaa ttgacatgta taatcgttcg gatgttgtta ttctacctac aaaagcgatg
     1921 catgattatc taattgaaaa agggatgact acctctaaag ttctttatca agaagtatgg
     1981 gatcatccag ttaatataga cctccctcgt ccagaatgtc aaaaagtcct tagttttgca
     2041 ggcgatattc aacgctttcc ctttgtcaat gactggaagg aaaatatccc gctcatctat
     2101 tatggagacg gaagcagact caattctgag gcaaatgttc atgctcaggg atggaaagat
     2161 gatgtagaat tgatgctaag tctttctaag cggggaggat ttggcctgtg ttggagtgaa
     2221 gatagagagg agttagtcga gcgtcgttat tcgaggatga atgcctcgta taaattatct
     2281 actttcttgg ctgctggact tccaattatt gctaaccatg atatttcaag tagagacttt
     2341 atcaaacaac acggattagg atttacagtt gaaacgctag aagaagctgt tgaaaaaatt
     2401 aataatatgg aaaaagagac ttatgacagc tatgtggaaa atgttgagaa gatagcaact
     2461 ctcttgcgaa atggttatat cactaaaaaa ttgttgatag atgctgtgca tatgctttat
     2521 agataggggt agcgatgaag aaaacaattg ttttaggtgc agatttccaa tatcgtgatc
     2581 aagttatgac aactataaaa tctattgtta gtcataatca acatttaact atttatatca
     2641 ttaacacaga ttttcccgta gaatggttta atatccttaa ccattcttta gaacagtttg
     2701 actgtagagt aaaaaatatt cccataagtt cagatgtctt tgaggggata cctaccctga
     2761 gccatatcag tgtggcggga tttttcagat ggtttattcc aattcacctt gaagaagaga
     2821 tagttttata tttagatagt gatgtaatag ttaggggttc tttggatcct ctatttgaca
     2881 taaaccttga agagaattta ttaggagctg tagcagatca tttttcaacg ctttactatg
     2941 gagatacagc accagttagt tttaattcag gtgttatgct aatcaataat tcattatgga
     3001 aaaaagaaga aatttataat agccttatga ggattgctga taagggttct gctgttgggg
     3061 taggagatca agaatacctt aatattttga ctcagaatcg ttggatagat atcggtaaac
     3121 aatataatgt tcagattggt caagatgtca atattaatgc atatgggcga ccagatttgt
     3181 atcattttta tgatgattgc gaacctgtta ttgttcatta taatagtcaa gataaaccgt
     3241 ggaataaata tagtcaatcg cgataccgtt cagaatggtg gtattatttt ggtttagaat
     3301 ggtcagttat ttatgctcaa caacaaaaaa atttgaacag gttaacaggt aaaacactga
     3361 atttatttac attaacagcc tcagatacat tagaacatat tgaagtactt gcaaaagctc
     3421 taccagatta tcagtttcat attgcagcac gtagtgatat ttcagagaaa ttagctaatc
     3481 tagaaactta tagcaatatt aaagtttata aattagttat caaccctatc ttggaagagc
     3541 ttatacgtaa ctgtgatgtg tacttggata ttaatcattc ttacgaagta gaaggtattc
     3601 tagataaaat aaaagaagag aataaaccga ttttagcctt tgattctaca gcacatacga
     3661 caaaaaatat agaagttatc tcagaaacaa tgcctgaatt gatggttaaa agaataaggg
     3721 aagtaggtcc tcttagatga aaaatagaaa agctatcgcg ttagcggcag attttgg
//
LOCUS       DATRMA010000110         3766 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.111, whole genome shotgun sequence.
ACCESSION   DATRMA010000110 DATRMA010000000
VERSION     DATRMA010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3766
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.111"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            54..1868
                     /gene="glmS"
                     /locus_tag="VBC28_001876"
     CDS             54..1868
                     /gene="glmS"
                     /locus_tag="VBC28_001876"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000334314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEO6815827.1"
                     /translation="MCGIVGVVGNTNATDILIQGLEKLEYRGYDSAGIFVVGDNKSQL
                     VKSVGRIAEIQAKVGDSVSGTTGIGHTRWATHGKPTEGNAHPHTSGSGRFVLVHNGVI
                     ENYLQIKETYLTKHNLKGETDTEIAIHLVEHFVEEDNLSVLEAFKKALHIIEGSYAFA
                     LIDSQDADTIYVAKNKSPLLIGLGNGYNMVCSDAMAMIRETSEYMEIHDKELVIVKKD
                     SVEVQDYDGNVIERGSYTAELDLSDIGKGTYPFYMLKEIDEQPTVMRKLISTYANESG
                     DMNVDSDIIKSVQEADRLYILAAGTSYHAGFAAKTMIEKLTDTPVELGVSSEWGYNMP
                     LLSKKPMFILLSQSGETADSRQVLVKANEMGIPSLTITNVPGSTLSREATYTMLIHAG
                     PEIAVASTKAYTAQVATLAFLAKAVGEANGKAEAKDFDLVHELSIVAQSIEATLSEKD
                     VISEKVEQLLISTRNAFYIGRGNDYYVTMEAALKLKEISYIQTEGFAAGELKHGTISL
                     IEDNTPVIALISADSTIAAHTRGNIQEVVSRGANALIIVEEGLEREGDDIIVNKVHPF
                     LSAISMVIPTQLIAYYASLQRGLDVDKPRNLAKAVTVE"
     gene            2031..2360
                     /locus_tag="VBC28_001877"
     CDS             2031..2360
                     /locus_tag="VBC28_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000056782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HEO6815828.1"
                     /translation="MSLPNCPKCNSEYVYEDGILLVCPECAYEWNPEEIEEEVGLIVL
                     DSNGTRLSDGDTVTVIKDLKVKGAPKDIKQGTRVKNIRLVDGDHNIDCKIDGFGAMKL
                     KSEFVKK"
     gene            2495..3136
                     /locus_tag="VBC28_001878"
     CDS             2495..3136
                     /locus_tag="VBC28_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEO6815829.1"
                     /translation="MSYILEILPSLLVGAQVTLQVFFIVICLSIPLGIVVAFLMKVHF
                     KPLQWLLTTYIWIMRGTPLLLQLIFFYYFLPSVGISFDRMPAAIIAFTMNYAAYFAEI
                     FRGGIESIPKGQYEAAKVLKFSKFQTVWYIVLPQVFKIVLPSVFNETITLVKDSSLVY
                     ILGVGDLLLESKTAANRDATLAPMFIAGGIYLLLIGLLTILSKQVEKRFNYYK"
     gene            3146..>3766
                     /locus_tag="VBC28_001879"
     CDS             3146..>3766
                     /locus_tag="VBC28_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009854102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEO6815830.1"
                     /translation="MLELKNISKCYGQKEIFKDFNLTVEEGKILSLVGPSGGGKTTLL
                     RMLAGLEKIDSGTIVHDGKEVSVDHLETLNLLGFVFQDFQLFPHLTVLDNLILSPVKT
                     MGLSKELAKEKALVLLERLGLKDHALVYPFSLSGGQKQRVALARAMMIDPQIIGYDEP
                     TSALDPELRQEVEKLILQNRETGMTQIVVTHDLQFAESISDTILKIN"
BASE COUNT         1230 a          570 c          758 g         1208 t
ORIGIN      
        1 aaagaaagtt tagatagaaa gtataagaga aagctcttat tggtaataat aatatgtgtg
       61 gtatcgtagg tgtagttgga aatacaaatg caacggatat tttaattcaa ggcttagaaa
      121 agctagagta ccgaggatat gactcagcag gtatttttgt agtaggtgat aacaaaagtc
      181 aacttgtgaa gtcagtcggt cgaatcgcgg agattcaggc aaaagtagga gattctgtta
      241 gtggtacgac aggtatcggt catacacgtt gggcaaccca tggtaaacct actgaaggaa
      301 atgcacatcc tcacacttca ggatctggtc gttttgtatt ggtacacaat ggtgttattg
      361 aaaattacct tcaaattaaa gaaacttact taacaaaaca taacctcaag ggtgaaactg
      421 atacagaaat agctattcat ttagttgaac actttgttga agaagataac ttatcagtgc
      481 ttgaagcctt taaaaaagcc ttacatatta tagaaggatc atacgcattt gctcttattg
      541 attctcaaga tgctgatacg atttacgtag ctaaaaataa atcacctctt ctaataggtt
      601 tgggaaatgg ttataatatg gtgtgttcag atgccatggc tatgatacgt gagacaagtg
      661 aatatatgga aatccacgat aaagaactcg ttattgttaa aaaagatagc gttgaagttc
      721 aagattatga tggtaatgtc attgaacgtg gtagctacac agcagagctt gacttatcag
      781 atattggtaa ggggacttat ccattctaca tgcttaaaga aattgatgag caaccaactg
      841 tgatgcgtaa gttgatttca acttatgcga acgaatctgg agatatgaat gtggattctg
      901 atattataaa atcagtccaa gaagcagatc gtctttacat attggctgca ggaacgtcat
      961 atcatgctgg ttttgctgct aagaccatga tcgaaaaatt aactgataca cctgttgaac
     1021 ttggagtttc ttcagaatgg ggttacaaca tgccactttt gagtaagaag ccaatgttta
     1081 ttttacttag tcaatcggga gaaacagcag atagtagaca agttttagtt aaagcaaacg
     1141 aaatgggaat ccctagttta actataacaa atgtgcctgg ctcaacactt tctcgtgaag
     1201 caacctatac gatgctcatt catgcaggac ctgaaatcgc tgttgcttct acaaaagcat
     1261 atactgctca agttgcaacg ttagctttct tagcaaaagc ggtaggagaa gcaaatggta
     1321 aggcagaagc aaaagatttt gatttggttc atgaattgtc aattgtagca caatcaattg
     1381 aagcgacctt atcagaaaaa gatgttatct ctgagaaggt agagcagttg ttaatctcga
     1441 cacgtaatgc cttctacatt ggtcgtggta acgactatta tgttacaatg gaggctgcac
     1501 tgaagctaaa agagatttct tatattcaga cagaaggttt tgctgcaggt gaattaaaac
     1561 atggtacaat ttcgttgatt gaagacaata ctcctgttat tgctttaata tctgctgatt
     1621 caactattgc agcgcatact cgtggaaata tccaagaagt tgtttctcgc ggagcaaatg
     1681 ctttgattat tgtagaagaa gggttggaac gtgaaggtga tgatattatt gttaataaag
     1741 ttcacccatt cttatcagcc atttcaatgg ttattccgac tcaattaatt gcttactatg
     1801 cttcattaca acgtggctta gatgttgata aaccacgtaa tttggcaaaa gctgtaactg
     1861 ttgaatagac tcgttacact aaaatacgct aatattaact acaaaatatt aaaaattgta
     1921 aaaaagccta tttttgaagg aaaataggct ttttcctatt gtcaagaagc ctataatcag
     1981 ttagaatgtt acttgctgac tattttatta attaatttaa ggagagaaaa atgtcattac
     2041 caaattgccc taagtgtaac tctgaatatg tctatgaaga cggtatttta ctggtttgtc
     2101 cagaatgtgc ttacgagtgg aatccagaag aaatcgaaga agaagtaggt ttaattgtat
     2161 tggatagcaa tggaacgcgc ttatcagatg gagatactgt gacagttatt aaggatttaa
     2221 aagtaaaagg agcgcctaag gatattaagc aaggcactcg tgttaaaaat attcgtttag
     2281 ttgatggcga tcataatatc gattgtaaaa ttgatggttt tggagctatg aaactaaaat
     2341 ctgagtttgt aaaaaaatag ctttcaggca attggcaacg gtcaattgct ttttgaatat
     2401 gacttgattt catgcatttt tattaaaaaa gctctatgac attggtttga attttgatat
     2461 aatgagaaca tctatttttt atttaggggg gaccatgtct tatattttag aaatcttacc
     2521 tagcttatta gtaggtgcgc aagtgacatt gcaagttttc tttattgtta tttgtttatc
     2581 aattccactt ggtattgttg tagccttcct aatgaaagtt cattttaagc cgcttcaatg
     2641 gctactgaca acttatattt ggattatgcg aggcacgcct ctgttattac agcttatctt
     2701 tttttattac tttttaccaa gtgtagggat ttcttttgat cgtatgccag ctgcgattat
     2761 tgcttttacg atgaattatg cagcttattt tgcagagatt tttcgaggag gtattgagtc
     2821 tattcctaaa gggcaatatg aagctgcaaa ggtattaaag ttttctaagt ttcaaacggt
     2881 ttggtacata gtattacctc aagtcttcaa aatagtctta ccgagtgttt tcaatgaaac
     2941 gattacttta gtgaaagatt cctcattggt ttacatactt ggtgtgggcg acttacttct
     3001 agaaagtaag acagccgcaa atagggatgc aacacttgca ccaatgttta tagcaggtgg
     3061 tatttaccta ttattaattg gtcttttaac tattttatct aaacaagttg aaaagcgttt
     3121 taactactat aaataaggag ggacgatgtt agaattaaaa aatatttcta aatgctatgg
     3181 gcaaaaagaa atctttaaag atttcaatct gactgtagaa gaaggtaaaa ttctctcttt
     3241 agtaggacct tctggaggtg gaaaaacaac actattacgt atgttagctg gattggaaaa
     3301 gattgattca gggactattg ttcacgatgg taaagaagta tctgttgatc atttggaaac
     3361 actaaatctt ttaggattcg tcttccaaga ctttcaatta ttcccacatt taacagtatt
     3421 agataatttg atcctctctc ctgttaaaac gatgggatta tcaaaagaac ttgctaaaga
     3481 aaaagcctta gtattacttg aacgcttggg acttaaagac catgctttag tctatccatt
     3541 ttcattatct ggggggcaaa agcaacgtgt cgcactagct cgtgcgatga tgattgatcc
     3601 acagattatt ggttatgatg agccaactag cgctcttgat ccagagttgc gtcaagaagt
     3661 agaaaaacta attttacaaa atagagaaac aggtatgaca caaattgtag taacacatga
     3721 tcttcaattt gctgaaagta tatctgatac gattctcaaa attaat
//
LOCUS       DATRMA010000111         3591 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.112, whole genome shotgun sequence.
ACCESSION   DATRMA010000111 DATRMA010000000
VERSION     DATRMA010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3591
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.112"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            complement(<1..367)
                     /locus_tag="VBC28_001880"
     CDS             complement(<1..367)
                     /locus_tag="VBC28_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HEO6815831.1"
                     /translation="MILTFNPGKLERQEFFKELINYLWIHDDVTLRKIKSHFTDYSKI
                     DRLLEEYINHGYILRQNKRYSLNLPFLSSLDGLVLDDLVFIDSDSQIYQLLQKRKFVT
                     NLDNPTNHLVFVEETDFERN"
     gene            complement(427..1362)
                     /locus_tag="VBC28_001881"
     CDS             complement(427..1362)
                     /locus_tag="VBC28_001881"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HEO6815832.1"
                     /translation="MSKILVFGHQNPDSDAIGSSVAFAYLAKEAWGLDTEAVALGTPN
                     EETAYVLDYFGVQAPRVVESAKAEGVETVILTDHNEFQQSISDIKDVTVYGVVDHHRV
                     ANFETANPLYMRLEPVGSASSIVYRMFKENGVSVPKELAGLLLSGLISDTLLLKSPTT
                     HASDIPVAKELAELAGVNLEEYGLEMLKAGTNLSSKTAAELIDIDAKTFELNGEAVRV
                     AQVNTVDINDILARQEEIEVAIQEAIVTEGYSDFVLMITDIVNSNSEILALGSNMAKV
                     EAAFEFTLENNHAFLAGAVSRKKQVVPQLTESYNA"
     gene            complement(1479..2267)
                     /gene="pflA"
                     /locus_tag="VBC28_001882"
     CDS             complement(1479..2267)
                     /gene="pflA"
                     /locus_tag="VBC28_001882"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HEO6815833.1"
                     /translation="MAEIDYKKVTGMIHSTESFGSVDGPGIRFIIFMQGCKMRCQYCH
                     NPDTWEMETNNSKERTVEDVLKEALRYKHFWGKDGGITVSGGEAMLQIDFITALFIEA
                     KKLGIHTTLDTCGFAYRATPEYHAILEKLLDVTDLVLLDLKEIDPEQHKIVTRQSNKN
                     ILQFARYLSDRGTPVWIRHVLVPGLTDIDDHLKRLGEFVQTLDNVDKFEVLPYHTMGE
                     FKWRELGIPYPLAGVKPPTPERVKNAKDIMKTESYTEYLKRIQN"
     gene            complement(2335..>3591)
                     /locus_tag="VBC28_001883"
     CDS             complement(2335..>3591)
                     /locus_tag="VBC28_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEO6815834.1"
                     /translation="SASEMALVSLNRSKVEQKAEEGDKRYRRLLDVLENPNNFLSTIQ
                     VGITFISLLQGASLSASLGHVISGWLGNSATARTAGSIIALIFLTYVSIVLGELYPKR
                     IAMNLKDRLAIVSAPIIIFLGKIVSPFVWLLSASTNLLSRITPMTFDDADEKMTRDEI
                     EYMLTNSEETLEAEEIEMLQGIFSLDEMMAREVMVPRTDAFMIDINNDAQSNIEGILS
                     QNFSRVPVFDDDKDRVVGVLHTKRLLEAGFKTGFDTIDLRKILQEPLFVPETIFVDDL
                     LKALRNTQNQMAILLDEYGGVAGLVTLEDLLEEIVGEIDDETDTAEQFVREIDENIYI
                     VLGTMTLNEFNDYFETELESDDVDTIAGYYLTGVGSIPNQEEKVAYEVDSKDKHITLI
                     NDKVKDGRITKLKVLLSDIEQNIEKD"
BASE COUNT         1148 a          720 c          548 g         1175 t
ORIGIN      
        1 tgtttctttc aaaatctgtc tcttctacaa ataccaagtg attggttggg ttatctaaat
       61 ttgtcacaaa ttttcgcttc tgtaataact gatagatttg actatcagaa tctatgaaaa
      121 ccaaatcatc taatactaat ccatctagcg aactcagaaa aggaagatta aggctgtagc
      181 gtttattttg gcgaaggata tatccatgat taatatattc ttctaacaaa cggtcaattt
      241 tagaatagtc tgtaaagtgg gactttattt ttcttagtgt tacatcatca tgtatccaaa
      301 gatagttaat aagctcctta aaaaactctt gtctttctaa ctttcctgga ttaaacgtta
      361 atatcataaa agcgctccta aatgtatgac taaaaaaacg ggacgcagcc cgttttacat
      421 gattaattaa gcattgtagc tttctgtcaa ttgaggaaca acttgttttt tgcgtgatac
      481 tgccccagca agaaaagcgt ggttattttc aagtgtaaat tcaaaagctg cttcaacttt
      541 agccatattt gaaccgagag ctaaaatctc tgaatttgaa ttaacaatgt cagtaatcat
      601 caatacaaaa tcagaataac cttctgtgac aattgcttct tgaatagcaa cttcaatctc
      661 ttcttgacgt gctaaaatat cgttaatgtc cacagtatta acttgtgcaa cacgaacagc
      721 ttctccattt aattcaaatg ttttagcatc aatatcaatc aactccgctg cagtcttact
      781 tgagaggtta gtacctgctt taagcatttc taaaccatat tcttctaagt ttacacctgc
      841 aagctctgct agttcttttg caactggaat atctgaagca tgagttgttg gtgatttcaa
      901 aagaagagta tctgaaatca aacctgataa taataaacca gccaactctt tgggtactga
      961 tactccattt tccttaaaca tacggtaaac aatcgatgat gctgagccaa ctggttccaa
     1021 acgcatatat aacggattgg cagtctcaaa gttagcaaca cgatgatgat caacaacacc
     1081 ataaactgtc acatctttaa tatcagaaat agattgttgg aattcgttat ggtcagttaa
     1141 aatgactgtc tctacaccct ctgcttttgc agattctact actcgaggtg cttgaacacc
     1201 aaaataatct aaaacatatg ctgtttcttc atttggagtt cctaaagcaa cggcctctgt
     1261 gtcaagcccc caagcttctt tagctagata tgcaaaagca actgatgatc caatagcatc
     1321 tgaatctgga ttttggtgac caaagactaa aattttagac atgtttttac ctcttctttt
     1381 atattcacct tattgtagca aaaattgaat aacttagcaa aaaatcttac taggaagccc
     1441 tttgcaacta aaaaagttga ataaaattca acttaaaact aattttgaat cctttttaga
     1501 tattctgtat aggattctgt tttcatgata tctttagcgt ttttaacacg ttctggagtt
     1561 ggtggtttca caccagctaa tggatacgga attcctaatt cacgccattt gaactctccc
     1621 atagtatgat aaggcaatac ttcaaattta tcgacattat caagtgtttg gacaaattca
     1681 cccaaacgtt ttaaatggtc atcaatatca gtcaatccag gaactaaaac atgacgaatc
     1741 cataccggag tacctctatc agataaatat ctagcaaact gtaaaatatt tttgttagac
     1801 tgacgagtta caattttatg ttgttctgga tcaatttctt ttaagtcaag gagtaccaaa
     1861 tcagtaacat ctaacaattt ttcaaggata gcatggtatt ctggtgttgc tcgataagca
     1921 aaaccacaag tatctagcgt cgtatgaata cctagtttct tagcctcaat aaagagggct
     1981 gtaataaagt caatttgcag cattgcttca ccacctgaaa ctgtgatacc tccatctttt
     2041 ccccaaaaat gtttataacg gagagcttcc tttagaacat cctcaacggt acgttccttt
     2101 gaattgttag tttccatttc ccaagtatca ggattatgac agtattgaca acgcatttta
     2161 catccttgca taaaaatgat aaaacgaata cctggcccat ccacagagcc gaaactttct
     2221 gtagaatgga tcattcctgt tactttttta tagtctattt ctgccatttc atcaccttgt
     2281 aaccgttttt atgactttat tatatcattt tcatgaaaaa aagactaagt aatcttagtc
     2341 tttttcaata ttctgttcta tatcagacaa tagcaccttc aattttgtta tacgaccatc
     2401 tttaacttta tcattaatca aagtgatgtg tttatctttg ctgtctactt cgtaagctac
     2461 tttttcctct tggtttggaa tactacccac accagttaaa taataacccg caattgtatc
     2521 aacatcatca ctttctaact ctgtctcaaa ataatcatta aattcattaa gcgtcatagt
     2581 tcctagaaca atatagatat tctcatcgat ttccctgaca aattgttcag ctgtatctgt
     2641 ttcatcatca atttcgccga caatttcttc caataaatcc tctagagtta ctaaaccagc
     2701 aacgccacca tattcatcta gtaaaatggc catttgattt tgagtgttgc gtaatgcttt
     2761 taaaaggtca tctacaaaaa tcgtttctgg aacaaaaaga ggttcctgaa gtatttttcg
     2821 caagtcgata gtatcaaaac cagttttaaa gcccgcttcc aataagcgct tggtatgtaa
     2881 aacaccaaca actctatctt tatcgtcatc aaaaacaggg acacgagaaa agttttgtga
     2941 taaaattcct tcaatatttg attgtgcatc attgttgatg tcaatcatga aagcatcagt
     3001 gcgcggaacc ataacttcac gcgccatcat ttcatctagc gagaaaatcc cttgcagcat
     3061 ctcaatttct tcagcttcca aagtttcttc actattcgtc aacatatatt caatttcatc
     3121 acgtgtcatc ttctcgtcag catcatcaaa agtcatcgga gtgattctac tcaataagtt
     3181 agttgaagct gagagcaacc atacaaaggg gcttacgatt ttccctagaa agataataat
     3241 gggagctgaa acaattgcta gacgatcttt caaattcata gcaatacgct taggatataa
     3301 ttctcctaga acaatagaga catatgttaa aaagattagc gcaataatac tccctgctgt
     3361 acgggctgtt gctgaatttc ctagccaccc tgaaatcaca tgtcctagtg aggcagataa
     3421 actagcccct tgcaaaaggc taataaaagt aataccaact tgaattgttg ataaaaaatt
     3481 attgggattt tcaagaacgt ccaaaagacg tcgatacctt ttgtcccctt cttcagcttt
     3541 ctgttctact ttagagcggt ttagcgatac taaagccatt tcagatgctg a
//
LOCUS       DATRMA010000112         3336 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.113, whole genome shotgun sequence.
ACCESSION   DATRMA010000112 DATRMA010000000
VERSION     DATRMA010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3336
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.113"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            161..508
                     /gene="rplS"
                     /locus_tag="VBC28_001884"
     CDS             161..508
                     /gene="rplS"
                     /locus_tag="VBC28_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEO6815835.1"
                     /translation="MNPLIQSLTEGQLRSDIPEFRAGDTVRVHAKVVEGTRERIQIFE
                     GVVISRKGQGISEMYTVRKISGGIGVERTFPIHTPRVDKIEVVRYGKVRRAKLYYLRA
                     LQGKAARIKEIRR"
     gene            619..690
                     /locus_tag="VBC28_001885"
     tRNA            619..690
                     /locus_tag="VBC28_001885"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:652..654,aa:Arg,seq:cct)
     gene            complement(653..1267)
                     /locus_tag="VBC28_001886"
     CDS             complement(653..1267)
                     /locus_tag="VBC28_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEO6815836.1"
                     /translation="MKFLETNEYLRLIKICKEKSQHQSYAALYLIAKTGMRFAECLGL
                     TVNDIDYTNKYLSINKTWDYHFNQRYLPTKNKSSIRNIPIDNDTLFFLHEFTKNKNDR
                     LFDKLSNNAVNKTIRKITGREVRVHSLRHTFASYLISISQVLDHENLNITLEVYAHQL
                     QEQKDRNDKLNQRNLGRIWGKIALNRYLHAMNMSPAGIEPAITP"
     gene            complement(1270..1536)
                     /locus_tag="VBC28_001887"
     CDS             complement(1270..1536)
                     /locus_tag="VBC28_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001881173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HEO6815837.1"
                     /translation="MAYFRKRDNGWEYRISYKHIIGNILLCNITSTKYQQVINHYSQT
                     HVQDTVKRFNIHVKACVSMAVHEGYIEKNFCLFTKVKAKNKGRK"
     gene            1536..1940
                     /locus_tag="VBC28_001888"
     CDS             1536..1940
                     /locus_tag="VBC28_001888"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEO6815838.1"
                     /translation="MIYSTLAKEQGVQGYLDGKGSLRDICKWYDISSRSVLQKWIKRY
                     TSGEDLKATSRGYSRMKQGRQATFEERVEIVNYTIAHGKDYQAAIEKFGVSYQQIYSW
                     VRKLEKNGSQGLVDRRVKGLESRPDLTEIEQL"
     gene            2102..2302
                     /locus_tag="VBC28_001889"
     CDS             2102..2302
                     /locus_tag="VBC28_001889"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEO6815839.1"
                     /translation="MPIQALCQLLKGSRSGYYKWLNRQKTDFETKNTKLMAKIKELHR
                     LYNGILGYRRMTTFINRQLGTT"
     gene            2324..2584
                     /locus_tag="VBC28_001890"
     CDS             2324..2584
                     /locus_tag="VBC28_001890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEO6815840.1"
                     /translation="MMNILGISSVIRRVSHACTKAGDRFYEENILNREFTATAHNQKW
                     CTDVTYLQYGLGAKAYLSAIKDLYNGSIIAYEISHNNEIHLL"
     gene            2710..2919
                     /locus_tag="VBC28_001891"
     CDS             2710..2919
                     /locus_tag="VBC28_001891"
                     /inference="COORDINATES: protein motif:HMM:NF025063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEO6815841.1"
                     /translation="MSRIGKCIDNAPTESFFGFFKTESYHLKKYNSYDELVNDVARYI
                     EFYNTQRYQSKLNNLTPLEFRNQVA"
     gene            complement(3092..3253)
                     /locus_tag="VBC28_001892"
     CDS             complement(3092..3253)
                     /locus_tag="VBC28_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NINE protein"
                     /protein_id="HEO6815842.1"
                     /translation="MGGIGAHKFYAGKIGQVFLYIIFSITFIPGIIDLIEALMALGKQ
                     TDEYGNIIV"
BASE COUNT         1057 a          575 c          639 g         1065 t
ORIGIN      
        1 aaataactat tctaaaggat gacaccttgt tcttctagat aaaatagctt tacaagtagg
       61 ttaaaatttg gtagaattgt gagagtgtgt gatggacgca caaaaaacgg ctaatccgct
      121 gagacaagta cttaagatta gtaagagaag gagaataaaa atgaatccat taattcaaag
      181 tttgacagaa ggtcaacttc gttctgatat ccctgagttc cgtgctggtg atactgtacg
      241 tgttcacgct aaagttgtcg aaggtactcg cgaacgtatt cagatctttg aaggtgttgt
      301 tatctcacgt aaaggtcaag gaatctcaga aatgtacaca gtacgtaaaa tttctggtgg
      361 tatcggtgta gagcgtacat tcccaattca cactcctcgt gttgataaaa tcgaagttgt
      421 tcgttatggt aaagtacgtc gtgctaaact ttactactta cgcgcattgc aaggtaaagc
      481 tgcacgtatt aaagaaatcc gtcgttaatt ttgatgatca gattttaaaa atgcttggtt
      541 gtttgaggat agtaactatg ttttaaaact ggacaaccaa gacgtaaaaa atctgcctgt
      601 gggcagtttt tttactaggt ccccttagtt caatggatat aacaactccc tcctaaggag
      661 taattgctgg ttcgattccg gcaggggaca tattcattgc atgtaaatag cggtttagag
      721 ctattttgcc ccaaatccgc cccaaatttc tctgattaag tttatcgttc ctatcttttt
      781 gttcttgtaa ttgatgtgcg taaacttcta aagtgatatt taaattctcg tgatctaaaa
      841 cttgagagat ggaaattaga tagcttgcaa atgtatgcct gagagagtgc actcgtacct
      901 cgcgaccagt tatttttcgg atagttttat tgactgcatt atttgaaagt ttgtcgaata
      961 atctgtcgtt tttatttttt gtaaattcat gcaaaaaaaa taatgtatca ttgtcaattg
     1021 gtatatttct gatactactt ttgttttttg ttggcaggta tctttggttg aaatgataat
     1081 cccaagtttt attaattgat aaatatttgt tagtgtaatc aatatcatta actgttaaac
     1141 ctaaacattc agcgaagcgc atgccagttt tagcgatgag gtataacgct gcatacgatt
     1201 gatgttgtga tttttcttta caaattttta tcaagcgtaa gtattcattg gtttcaagaa
     1261 attttatctc tatttacgcc ccttattttt tgctttaacc ttagtgaata aacaaaaatt
     1321 tttttctata tatccctcgt gaacagccat ggatacgcag gcttttacat gtatgttaaa
     1381 acgctttact gtatcttgca catgcgtttg actataatga tttatgactt gttgatattt
     1441 agtggaagta atattgcaaa gtaatatatt tcctattata tgtttatacg atattcgata
     1501 ttcccacccg ttgtcgcgtt tacggaaata cgccattgat atactccaca ttagctaaag
     1561 aacagggtgt tcaaggctac cttgatggaa aaggctctct tagagatatt tgtaaatggt
     1621 atgatatctc aagtcgctct gttctccaaa agtggataaa acggtatact agtggtgaag
     1681 acttgaaagc cactagtaga ggatatagcc gtatgaaaca aggaaggcaa gccacatttg
     1741 aagaacgtgt agagattgtt aactacacca ttgcccatgg gaaagactat caagcagcta
     1801 ttgagaagtt tggtgtttcc taccaacaaa tttattcttg ggtgcgtaag cttgagaaga
     1861 atggctcaca aggtttggtt gatagacgtg tgaaagggtt ggagagtagg cctgatttaa
     1921 ccgagattga gcaactttaa ctcaagatta aacaattgga ggaacgtaat cgtctcttag
     1981 aaatcgaggt tagtttacta aaaaagttag aagacatcaa acgaggaaac agacggtaag
     2041 actaggtaag catttagcgg agttccaagt aatcaagaat tattacgatg aggaatctaa
     2101 tgtgcctatt caggccttat gccaactctt gaaggggtct cgttcaggct attacaagtg
     2161 gctcaatcgt caaaaaacag attttgagac aaaaaataca aagctaatgg ctaaaatcaa
     2221 ggaacttcat agactctaca atggtatctt aggttatcgc cgtatgacaa catttattaa
     2281 tcgtcaactt gggacaactt aaaacaagaa acggattcgt tgattgatga acattctggg
     2341 gattagttca gtcattcgtc gtgttagcca tgcttgtaca aaagctggtg acagatttta
     2401 cgaagaaaat attcttaatc gtgaatttac agccacagct cataaccaga aatggtgcac
     2461 agatgtcacc tatcttcaat acggtctggg agctaaagct tatctcagtg cgattaaaga
     2521 cctgtataac ggttctatta tcgcttatga gattagtcac aacaatgaaa tccacttgtt
     2581 atgaagacca ttaaaaaggg gctagagctc aatccaggag ccacacctat catccatagc
     2641 gattgaggta gtcaatatac ttccaaagaa taccgttata tcatacaaca agctggtctg
     2701 accttatcca tgtcccggat tggcaaatgt attgataatg caccaactga aagtttcttt
     2761 gggtttttca agactgagtc ttaccacctt aagaaataca actcttatga tgagttggtc
     2821 aatgatgtgg cacgttatat cgaattctac aacacacaac gttatcaatc aaaattaaac
     2881 aacctgactc ctctagaatt caggaatcag gttgcataac ttatctttta ttatttgact
     2941 gtctacttga cagggagccg ttcagattgc ttaacctttc taaatttgct aaaatagcta
     3001 caagaaaacg agccatttaa tgcttatttc ttatactgtc ttgcctcacg ctctcctcga
     3061 ccaaaaattg agcgtgaggc tttttgtttc attaaacgat gatatttcca tattcatcag
     3121 tttgttttcc gagagccatc aaagcttcga taaggtcgat aattccagga ataaaggtaa
     3181 tactaaaaat aatatataaa aaaacctggc ctatttttcc tgcgtaaaat ttatgcgctc
     3241 caatgccgcc caaaagaacg ttaataaaac ataaactact atgttagcat aagactttat
     3301 ttttacaact gaatttcata taaatggatt agagta
//
LOCUS       DATRMA010000113         3323 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.114, whole genome shotgun sequence.
ACCESSION   DATRMA010000113 DATRMA010000000
VERSION     DATRMA010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3323
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.114"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            307..765
                     /locus_tag="VBC28_001893"
     CDS             307..765
                     /locus_tag="VBC28_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6815843.1"
                     /translation="MRKYIKWLIPISIFGMILGGCQMNSEHKSQSNEVKNSKQSEVKK
                     DKKMTKKEQLAYLKEHEQEIIDYVKLHNNQIESVQFDWSSVKVEQSGNGTPQGGDYNL
                     SLRGKFNHLQNSKLIVDFYLAHKNDIPNIKSMGMLNKPYIHKNGIWHIYE"
     gene            829..2462
                     /locus_tag="VBC28_001894"
                     /pseudo
     CDS             829..2462
                     /locus_tag="VBC28_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073524.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            2481..2753
                     /locus_tag="VBC28_001895"
     CDS             2481..2753
                     /locus_tag="VBC28_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6815844.1"
                     /translation="MSKELKLPLNEMLKEQERIAIKAIVATAKSTITDTGTNLKATLE
                     SDAQDLKNYASTYNANITDGLEGQSAQAASDFLTQLPKPELDNPVN"
     gene            2763..3113
                     /locus_tag="VBC28_001896"
     CDS             2763..3113
                     /locus_tag="VBC28_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6815845.1"
                     /translation="MIDTKKLQELDQEYDQNLRNIYRNREQLEDDFHLFMARTDSLKE
                     SVYQATLGQGWELPQEAHAHLYNMDDNKDTFISEFNEYMEKLEEKEIDLRRVYNDRVD
                     ELYQKAKQNEAKKG"
BASE COUNT         1270 a          524 c          583 g          946 t
ORIGIN      
        1 ttataaagac aaggtttcta aaccttgtct tttttctact agataattta ttgtgaaatg
       61 ttacttgtaa tatatattta tttttttcat tgtagaagaa tttatataat aataaatatt
      121 aattattaat ttaattaggg catttaaaat atacaaatga ttttctgttt acatgttaag
      181 actaaaacta taaaaatacg tgaaaatttt gttatactaa aattaatttt ataatgttgg
      241 tgaataatag ttaatttcat tatatacttg atatataaaa tttatattct aagaaaagag
      301 aacatgatga gaaaatatat aaaatggtta attcccatat caatttttgg gatgatactt
      361 ggaggctgtc aaatgaatag tgaacataaa agtcagtcaa acgaagtaaa aaatagcaag
      421 caatcagaag tgaagaaaga taaaaaaatg acaaaaaaag aacaattagc ctatctcaaa
      481 gagcatgagc aagaaatcat agattatgta aaattacata acaaccaaat tgagtccgtt
      541 caattcgatt ggtcaagtgt aaaagtagaa caaagcggga atggaactcc acaagggggt
      601 gattataatc tttcactgag aggaaagttt aatcatctac aaaattcaaa attaatagtt
      661 gatttttatt tagctcataa aaatgatatc ccaaatatca aatcaatggg aatgctaaat
      721 aagccgtata tacataaaaa tggtatttgg cacatttatg aatagcttga atcatcctat
      781 ataaagtaat agaattttta ccacaagcaa ggtagaggag aagaataatt gagtaattgg
      841 aacttagata attttgataa cttacatagc tctttagcag aaagtgcata taatagtcga
      901 cctaattcat ttcctgaatt atttgagact gactcagtaa cagaagtaaa attttctcaa
      961 ccctcagaag ataacaaagg tcaaatcaca cagggtggca ctaacctccc caatgatggt
     1021 attgtctacc tgcaaccaga taagtcctta aaatctattg atgaaaatgt aaaggtcctc
     1081 attccagatg tcaatggagg ctatcacact gagcattatg ttactcattc ttatcaaaaa
     1141 ggtgtgctta ctgacgataa ggcaggtttt aatgcttact acctgtcaga tactgaaaaa
     1201 atagatagca ctacaaagca cacttatcta gccattcgtg gtagtgatgg gattggtcta
     1261 gatacactaa acgattgggt tagtaacaat gcaatgtttg ctgtctctaa caagtatatt
     1321 ccacaagcga aattggctaa taaggctatg aaagaaaaaa ttgccgagct aaaaggaaaa
     1381 gcgcctggag ctattataga tgttactggt cactcactcg gtacgattgt atcttctcaa
     1441 gcagttgtta atcttagcta tgcagaattg gagaatgttg gtcaagtagt ccttttcgat
     1501 ggccctgatg tatcaagaag cttggaaaaa atggaaggca tttctgctaa aaaatccaag
     1561 aagctgggaa gcatgttacc tactatgtca atccctttga tatagttagt atgcttaatc
     1621 gtgaaaagcc atggaaagac caattcggga aagtaaatgt cattgttcct agtaaatata
     1681 cgggtacttt tgatgagcaa tcttctcacg attttggtgc cttccaaatt gatagtagcg
     1741 gaaatccttt agttgcttct gaaagctatc atccagaatt attaacatct ggtcaagact
     1801 tagctagact cgaaaaaaaa ttcataaaga atcttttagg agagcattat gattcggata
     1861 agctaggacc agtactcaaa ggcgctctga ctggggacct gactcgacta attgcaacat
     1921 taaaagcttg tggtatcagt gtcactgtaa acgaagctaa aaaactgtac gataacttcc
     1981 aaaaagacta caaagccata atttccaaag ctaaaaaaga tggcctcgag tgggatcgca
     2041 aaaacatcgc aagcttccat agtaggatca aatcagcatc aggcagtcaa cggattttac
     2101 ttagaactca aatgctctat atggcaacgc aattagcaga atcagatgtt tcagaaaaag
     2161 tctctgcaac aaaaaaacat attagtgaag ctaaggatac gattgtagaa atatcgacat
     2221 ccactatttc atcagctgaa ataatggcta tgcaccttga ccaatcagaa gttgatgctc
     2281 tggtttcaga tataaaaatg tcaacagttt ggaatgatgg cgtagagact tctgattatg
     2341 aagcacttga tcactataaa acaaaaatga ccacatttac gaccaatttg gtaacagttg
     2401 cacaaaattt aacagctcaa gatgagcaac tagctggtga tattgtcacg aacttatcct
     2461 aaaatagaga gattaaatga atgtccaaag aattgaaatt gcccttgaat gaaatgttga
     2521 aagaacaaga aagaatagct attaaggcaa tagttgctac agcaaaaagc actattacgg
     2581 atactggaac aaatttaaaa gcaactttag agtcagatgc ccaagaccta aaaaactatg
     2641 catccaccta taacgccaat atcacggatg gtcttgaagg ccagtcagct caagcagcat
     2701 ctgatttctt gacacaactt ccaaaaccag agttagataa tccagtcaat taggaggcta
     2761 gtatgataga tacaaaaaaa ctgcaagaac tcgatcaaga atatgatcaa aatctcagaa
     2821 acatttatcg taacagagag caactagaag atgactttca cttgtttatg gcaagaacag
     2881 acagcctaaa agagtctgtc tatcaagcaa ctttaggaca aggttgggaa ttaccccaag
     2941 aagctcatgc acacctatat aatatggatg ataataagga tacattcata tcagaattca
     3001 atgaatatat ggaaaagctt gaagaaaagg aaatagatct tcgaagagtt tataatgata
     3061 gagtagatga attgtatcag aaggctaagc aaaacgaagc aaaaaaagga taaaaatttt
     3121 ttcaagtaat atatataaat atgaaaaata aagaatcttg cagtaaaatg agtatttatt
     3181 tttcacgacg aacagtcact acaaaatcac taaaatctca aaattacttg gtttttaagt
     3241 taatgtctaa tactcaaacc ttgttaaatc aatgtttgtt atctttaaaa agtgctcagt
     3301 agcactcaaa atccattgtc ctc
//
LOCUS       DATRMA010000114         3278 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.115, whole genome shotgun sequence.
ACCESSION   DATRMA010000114 DATRMA010000000
VERSION     DATRMA010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3278
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.115"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            37..843
                     /locus_tag="VBC28_001897"
     CDS             37..843
                     /locus_tag="VBC28_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000705139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEO6815846.1"
                     /translation="MKITLHGVAETLLITLYIRAKDAMAKHPILNDQKSLAIVEQIEY
                     DFDKFDNSEASFYATLARIRVMDREIKKFIRENPNSQILSIGCGLDTRFERVDNGQIR
                     WYNLDLPEVMEIRKLFFEEHERVTNIAKSALDETWTREVNPQNAPFLIVSEGVLMFLK
                     EDDVETFLHILTNSFSQFMAQFDLCHKEMINKGKQHDTVKYMDTEFQFGITDGHEIVD
                     LDPKLKQINLINFTDEMSKFELGTLRSLLPTIRKFNNCLGVYEYKASEKK"
     gene            1238..1735
                     /gene="ybeY"
                     /locus_tag="VBC28_001898"
     CDS             1238..1735
                     /gene="ybeY"
                     /locus_tag="VBC28_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEO6815847.1"
                     /translation="MYVEMIDETGQVSEDIKKQTLDLLEFAAQKTGKENKEMAVTFVT
                     NERSHELNLEYRDTDRPTDVISLEYKPEVDISFDEEDLAENPELAEMLEDFDSYIGEL
                     FISIDKAKEQAEEYGHSYEREMGFLAVHGFLHINGYDHYTPEEEKEMFSLQEEILTAY
                     GLKRQ"
     gene            1716..2114
                     /locus_tag="VBC28_001899"
     CDS             1716..2114
                     /locus_tag="VBC28_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000365222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEO6815848.1"
                     /translation="MDLNDNNQKKWKNRTLTSSMEFAVTGIFTAFKEERNMRKHLVSA
                     ILVILAGLTFQVSMVEWLFLLLSIFLVITFEIINSAIENVVDLASNYHFSMLAKNAKD
                     MAAGAVLVVSLFAVLVGLIIFIPKILALLL"
     gene            2156..3055
                     /gene="era"
                     /locus_tag="VBC28_001900"
     CDS             2156..3055
                     /gene="era"
                     /locus_tag="VBC28_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012658024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEO6815849.1"
                     /translation="MTFKSGFVAILGRPNVGKSTFLNHVMGQKIAIMSDKAQTTRNKI
                     MGIYTTETEQIVFIDTPGIHKPKTALGDFMVESAYSTLREVETVLFMVPADEKRGKGD
                     DMIIERLKAAKIPVILVINKIDKVHPDQLLEQIDDFRSQMDFKEVVPISALQGNNVPT
                     LIKLLTDNLEEGFQYFPEDQITDHPERFLVSEMVREKVLHLTQQEVPHSVAVVVESMK
                     RDEETDKVHIRATIMVERDSQKGIIIGKQGAMLKKIGKMARRDIELMLGDKVYLETWV
                     KVKKNWRDKKLDLADFGYNEKEY"
BASE COUNT         1140 a          444 c          644 g         1050 t
ORIGIN      
        1 atttctaagt aaaatagcat aaaggagagt agatgaatga agataacatt acacggcgtt
       61 gctgaaacgt tattaatcac cttatatatt agggctaaag atgcgatggc taaacatccg
      121 atacttaatg atcaaaaatc cttagcaatt gttgaacaga tagaatatga ttttgataaa
      181 ttcgataatt cagaagcttc tttttatgca acattagcta gaattcgcgt tatggataga
      241 gaaatcaaaa aatttattag agaaaatcca aatagtcaaa tcctttcaat tggttgtgga
      301 cttgatacaa ggtttgaaag agtcgataat ggacaaatta ggtggtataa ccttgatttg
      361 ccagaggtta tggagataag aaaattattt tttgaagagc atgaaagagt tactaatata
      421 gcaaaatcag ccctagatga aacttggaca cgggaggtaa atccccaaaa tgcccctttt
      481 ctaatcgtgt cagaaggtgt tttaatgttt ctaaaagaag atgacgtaga gacttttctt
      541 catatcctga caaattcatt tagccaattt atggcacaat ttgatttgtg tcataaggaa
      601 atgattaata aaggaaagca acatgataca gtaaagtata tggatacaga atttcagttt
      661 ggtatcacag atggtcatga gattgtggat ttagacccta aattaaagca aataaatctg
      721 attaacttta cagatgagat gagcaaattt gagttaggca cacttcgctc tttacttcca
      781 acaattcgta aatttaataa ttgtttaggt gtgtacgaat ataaagcatc tgagaaaaag
      841 taacaaaaat ctatttatat aaagagacat tctaaatgta aatttgtcat aatcgttata
      901 gcatttgtag ctttgtttat ttaggaatta gttttacgta agttaggcgc tctaaagttt
      961 gtattttgaa gtaaatacta attcataagt cagaatttta ttgaaatgga ttataagtgt
     1021 gtaatagtga ttattagctt tctctccctt ttttaatact cttatggtat aatgatgtga
     1081 aagaatagct tgaaggaagg aagagaacgc acttttattt tgagaactag ttttagataa
     1141 aagtgaaaaa cttaggaaag tagatgtgtc gattatttag aattatgcat tacactttat
     1201 cttttctaaa cgttttgggt cgttcttact atataagatg tacgttgaaa tgattgatga
     1261 aactggacaa gtttcagaag atatcaaaaa gcaaactctt gatttgttag agtttgcagc
     1321 acaaaaaaca ggtaaagaga ataaagaaat ggctgtaacg tttgtcacta atgaacgtag
     1381 ccatgaattg aatttagagt atcgagatac cgatcgtccg actgatgtta tcagtttaga
     1441 atataagcct gaggttgata tttcttttga tgaagaagat ctggctgaaa atcctgaact
     1501 tgctgagatg ctagaagatt ttgattctta tattggtgaa ctttttattt ctattgataa
     1561 agctaaagaa caagcagaag agtatggtca ctcttatgaa cgtgaaatgg gttttttagc
     1621 tgtacatggt tttttgcata ttaacggtta tgatcattat acacccgaag aagaaaaaga
     1681 gatgttcagc ttacaggaag aaattttaac tgcttatgga cttaaacgac aataatcaaa
     1741 aaaaatggaa aaatcgcacc ttaacatcaa gcatggaatt tgctgttaca gggattttta
     1801 cagcttttaa agaagaaaga aatatgcgta agcatttggt ttctgccatt ttagtaatac
     1861 ttgctggtct tactttccaa gtatctatgg tggagtggtt atttctttta ttgagtattt
     1921 ttttggtaat tacttttgaa attatcaatt cagctattga aaatgtagtt gatttggcaa
     1981 gtaattacca tttctcgatg ctggctaaaa atgctaaaga tatggcagca ggagcggttt
     2041 tagttgtttc cttatttgcc gtattggtgg gattgattat ttttataccc aagatacttg
     2101 ctttactttt atgatgagaa gtattacgaa taaattgaat ggagaaagaa aagaaatgac
     2161 atttaagtca ggatttgtag caattttagg tcgtccaaat gttggaaaat caaccttcct
     2221 aaaccatgtt atgggacaaa aaattgctat catgagtgat aaagctcaaa caacacgtaa
     2281 taaaattatg gggatttata caacagaaac tgagcaaatt gttttcattg ataccccagg
     2341 aattcataaa ccaaagaccg cccttggtga ttttatggtt gaatctgctt atagtacttt
     2401 acgtgaagtt gaaacagttc ttttcatggt tcctgccgat gaaaagcgag gcaaaggcga
     2461 tgatatgatt attgaacgtc ttaaggctgc caagattcct gttattttag taatcaacaa
     2521 aattgataag gttcatccag accaactttt ggaacagatt gatgacttcc gtagtcaaat
     2581 ggattttaaa gaagtagtgc caatttctgc ccttcaagga aataatgtgc caaccctaat
     2641 caagttattg actgataatc ttgaagaagg tttccaatat ttcccagaag atcagattac
     2701 tgatcatccg gaacgatttt tagtatcaga aatggttcgt gagaaggtac ttcatttaac
     2761 gcaacaagag gttcctcact cagtagctgt tgttgtcgaa tccatgaaac gtgatgaaga
     2821 aactgataaa gtgcatattc gtgcaactat catggttgaa cgtgatagtc aaaaaggcat
     2881 tatcattgga aaacaaggtg ctatgctcaa gaaaatagga aaaatggcgc gccgtgatat
     2941 tgaacttatg ttaggtgata aagtttacct agagacttgg gtaaaagtca agaaaaactg
     3001 gcgtgataaa aagcttgatt tagctgattt tggctataat gaaaaagagt attaatgagt
     3061 ttattttgag atccgcaagt tagtggggaa gagttagata agttcaccgt gagacttgat
     3121 tcgcacagaa aaagcaattc acgaggataa aaccaactag tcataatttt gagattatat
     3181 tcacaaatta attaaaggga gccaaacatg cttgttggcc ccttcttaaa taaaggagag
     3241 cacatgactc aagattatat ttgctaccta taaagtag
//
LOCUS       DATRMA010000115         3263 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.116, whole genome shotgun sequence.
ACCESSION   DATRMA010000115 DATRMA010000000
VERSION     DATRMA010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3263
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.116"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            102..878
                     /locus_tag="VBC28_001901"
     CDS             102..878
                     /locus_tag="VBC28_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001932415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEO6815850.1"
                     /translation="MKLTTLGCWGGYPYKDGGTTSFLLTGENNFHLLIDAGSRSINEL
                     EKEISPLDLDAVIISHYHPDHVADLGVLYHYRLLYPKDKWEPKVLPVYGHNEDDYEFS
                     KLTLPNVAQGIAYHTNGVEQVGPFNITFIKTVHPVVCYAMRITERETGQVLVFTGDSG
                     YFADLVPFTKGADLFLADVYLYEGNENHIAHLTSKEAGEIAYNAKVKKLVLTHVPQFP
                     GEGINPENHLEELRLQSEKYAGGISVDLAQPHKSWDLGKL"
     gene            878..1384
                     /locus_tag="VBC28_001902"
     CDS             878..1384
                     /locus_tag="VBC28_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001169006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEO6815851.1"
                     /translation="MQKFTQEEKEWFMTEALKEAKSSLEKEEIPIGCVIVKDGHIIGR
                     GHNAREEFNKAILHAEIMAINNANEKVGNWRLLDTTLFVTVEPCVMCSGAIGLARIPH
                     VIYGAKNTKFGAAGSLYDILSDSRLNHRVEVETGILEDQCAKIMQDFFRKGRQRKKEA
                     KLSEKTKI"
     gene            1419..1504
                     /gene="ffs"
                     /locus_tag="VBC28_001903"
     ncRNA           1419..1504
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VBC28_001903"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            1656..3005
                     /locus_tag="VBC28_001904"
     CDS             1656..3005
                     /locus_tag="VBC28_001904"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEO6815852.1"
                     /translation="MTHITFDYSKVLGQFVGEHELDYLQPQVSAADAFLRQGTGPGSD
                     FLGWMDLPENYDKEEFSRIQKAAEKIKSDSEVLVVIGIGGSYLGAKAAIDFLNNHFAN
                     LQTAEERKAPQILYAGNSISSTYLADLVEYVQDKEFSVNVISKSGTTTEPAIAFRVFK
                     ELLVKKYGQEEANKRIYATTDKVKGAVKVEADANNWETFVVPDNVGGRFSVLTAVGLL
                     PIAASGADITALMEGANAARKDLSSDKISENIAYQYAAVRNVLYRKGYITEILANYEP
                     SLQYFGEWWKQLAGESEGKDQKGIYPTSANFSTDLHSLGQFIQEGYRNLFETVVRVEK
                     PRKNVTIPELTEDLDGLGYLQGKDVDFVNKKATDGVLLAHTDGGVPNMFVTLPTQDAY
                     TLGYTIYFFELAIGLSGYLNSVNPFDQPGVEAYKRNMFALLGKPGFEELSAELNARL"
BASE COUNT         1105 a          516 c          655 g          987 t
ORIGIN      
        1 aaaatttaga tttactagtc ttttttgtgc ataaaatcta aaacagttat tcttaataaa
       61 atttggtata atagcattaa atcagtaagg aggggattat catgaaatta acaacgttag
      121 gttgttgggg tggttatcct tataaagatg gaggaaccac aagttttcta ttaactggag
      181 aaaataattt tcacttatta atagatgcgg gaagtcgttc aatcaatgag ctagaaaaag
      241 aaataagccc gttggatttg gatgcagtta tcatcagtca ttatcatcca gatcatgtag
      301 cagatttggg tgtgttgtat cattatcgtt tgctataccc taaagataaa tgggaaccca
      361 aagtattgcc agtttatgga cataatgagg atgattatga gttttctaaa ttgacattac
      421 ctaacgttgc tcaaggaatt gcttatcata ccaatggagt tgagcaagtt ggtcccttca
      481 atattacgtt tatcaaaaca gtccacccag tcgtctgcta tgccatgagg attacagaaa
      541 gagaaactgg tcaagtatta gtctttacag gagattctgg gtattttgct gaccttgtac
      601 cttttacaaa aggcgcagat ttatttttag cagacgttta tctttacgag ggaaatgaaa
      661 atcatattgc acatttgact agtaaagaag caggtgagat tgcttataac gctaaagtta
      721 agaaattagt tttaacacat gttccgcagt ttccgggaga aggaataaac cccgaaaatc
      781 atttggaaga acttcgacta cagtctgaaa agtatgcagg tggcatctct gttgatttag
      841 ctcagccaca taaatcttgg gacttaggaa aactataatg cagaaattta cccaagaaga
      901 aaaagaatgg tttatgacag aagctctaaa ggaggcaaaa tcctccttag agaaagaaga
      961 aattcctata ggttgtgtca ttgtaaaaga tggtcatatt attggaagag gacataatgc
     1021 tcgtgaagag tttaataaag ctattttaca tgctgaaatc atggctatta acaatgcaaa
     1081 tgagaaggta gggaattggc gcttattaga tactacttta tttgttactg tagaaccttg
     1141 tgtaatgtgt agtggagcta ttggcttagc tagaattccc cacgttatat atggcgctaa
     1201 gaatacgaaa tttggagcag cgggaagtct ctatgatatt ttaagtgata gccgacttaa
     1261 tcatagagtt gaggttgaaa ccggcatttt agaagatcaa tgcgctaaaa taatgcagga
     1321 tttctttcga aaaggacgac aacgcaagaa agaagctaaa ctaagtgaaa aaactaagat
     1381 ttgatagctt gcaataaaat attagttttg ttataatatc tttggagcaa catttactcg
     1441 tgaagtgggt caggggagga atccagcagc cctaagcgat gctaaatgtg tgctcttttt
     1501 attttcaaaa gtcaaagtgt ttttggaaat aattatgaaa gtttcataaa aattggtagc
     1561 gtttcaaaaa atatgaaaat acagttaaaa aaccttgaaa aaaaactcac aaaagagtaa
     1621 gatgatagtg aaacataatt gttaggagaa ataaaatgac acatattaca tttgactatt
     1681 caaaagtctt gggtcaattt gtaggcgaac acgagttaga ctacctacaa ccacaagtaa
     1741 gtgcagcaga tgctttcctt cgtcaaggga ctggtcctgg ctcagatttt ctcggatgga
     1801 tggacctccc agaaaactat gacaaagaag aattttctcg cattcaaaaa gccgctgaaa
     1861 agattaaatc agatagcgaa gtactcgtgg ttattggtat tggtggttcg taccttggcg
     1921 caaaagcagc aattgacttt ttgaataatc attttgctaa tttgcaaacc gcagaagaac
     1981 gtaaagcgcc tcagattctt tatgctggaa attctatttc atctacttac cttgccgatt
     2041 tagttgaata cgtccaagat aaagaattct cagtaaatgt catttcaaaa tcaggtacaa
     2101 caactgaacc agcgattgct ttccgtgtat ttaaagaact tctagttaaa aagtacggtc
     2161 aagaagaagc taataaacgt atctatgcta caactgataa agttaaaggt gccgtaaaag
     2221 ttgaagcaga tgctaacaat tgggaaacat ttgtagttcc tgataatgtt ggtggtcgtt
     2281 tctcagtact gacagccgta ggtcttcttc caattgctgc ttcaggagca gatattactg
     2341 ctcttatgga aggcgcaaat gctgctcgta aagatttatc ttcagataaa atctctgaaa
     2401 acattgctta tcagtacgct gcagttcgta atgttcttta tcgcaaaggt tatatcacag
     2461 agattttagc taactacgaa ccatcattac aatactttgg tgaatggtgg aaacaattag
     2521 caggtgaatc agaaggtaaa gatcaaaaag gtatctatcc tacatcagca aacttctcaa
     2581 ctgatcttca ctcacttggt caatttattc aagaaggtta ccgtaacctc tttgagacag
     2641 ttgttcgcgt tgagaaacct cgtaaaaatg tgactattcc tgaattaacc gaagatttag
     2701 atggtcttgg ttatcttcag ggaaaagatg tagattttgt taataaaaaa gcaacagatg
     2761 gtgtactcct tgctcataca gatggtgggg ttccaaatat gtttgtaacg cttcctacac
     2821 aagacgctta cacccttggt tacactattt acttctttga gttagcaatt ggcctttcag
     2881 gttatcttaa ctcagtaaat ccatttgatc aaccaggggt agaagcatat aaacgtaata
     2941 tgtttgctct tctaggtaaa cctggattcg aagagcttag cgctgaattg aatgcacgtc
     3001 tttaaaaata tctaactaaa aagttcctca aagagaggaa ctttttagtt ttgttgtagg
     3061 ataactaaaa acccactagc accggtagcg atagtgggtt ggaaaaaggg ttgagaaaag
     3121 ttattaactt ttctcaggag atttatgaaa aaggaaaata atttaggata atattttcct
     3181 aacggctagg ataaaccgct tattttagga tgatactagt atacagtaga aaccttaaat
     3241 aaaccttaaa aattcatact tta
//
LOCUS       DATRMA010000116         3215 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.117, whole genome shotgun sequence.
ACCESSION   DATRMA010000116 DATRMA010000000
VERSION     DATRMA010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3215
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.117"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            complement(45..1319)
                     /locus_tag="VBC28_001905"
     CDS             complement(45..1319)
                     /locus_tag="VBC28_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HEO6815853.1"
                     /translation="MKFNEQSNSQAALLGLQHLLAMYAGSILVPIMIASALGYNAEQL
                     TYLIATDIFMCGIATLLQLQLSKHFGVGLPVVLGCAFQSVAPLSIIGAQQGSGYMFGA
                     LIASGIYVVLVAGIFSKVANFFPPIVTGSVITTIGLTLIPVAMGNMGDNAKEPSLQSL
                     TLSLVTIGVVLLINIFAKGFLKSISILIGLISGTILAAFMGLVDASVVAEAPLVHIPK
                     PFYFGAPRFEFTSILMMCIIATVSMVESTGVYLALSDITNDKLDSKRLRNGYRSEGLA
                     VLLGGLFNTFPYTGFSQNVGLVQISGIRTRKPIYFTALFLVILGLLPKFGAMAQMIPS
                     PVLGGAMLVLFGMVALQGMKMLNQVDFEHNEHNFIIAAVSIAAGVGFNGTNLFISLPN
                     TLQMFLTNGIVISTLTAVVLNIILNGLPKKLI"
     gene            complement(1319..1900)
                     /locus_tag="VBC28_001906"
     CDS             complement(1319..1900)
                     /locus_tag="VBC28_001906"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HEO6815854.1"
                     /translation="MKLLEERILKDGDVLGENILKVDSFLTHQVDFELMQEIGKVFAD
                     KYKEAGITKVVTIEASGIAPAVYAAQALGVPMIFAKKAKNITMTEGILTAEVYSFTKQ
                     VTSQVSIVSRFLSNDDTVLIIDDFLANGQAAKGLLEIIGQAGAKVAGIGIVIEKSFQD
                     GRDLLEKTGVPVTSLARIKAFENGRVVFAEADA"
     regulatory      complement(2004..2099)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(2155..3138)
                     /gene="guaC"
                     /locus_tag="VBC28_001907"
     CDS             complement(2155..3138)
                     /gene="guaC"
                     /locus_tag="VBC28_001907"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HEO6815855.1"
                     /translation="MFNDIPVFDYEDIQLIPNKCIISSRSQADTSVKLGNYTFKLPVI
                     PANMQTIIDEEVAETLACEGYFYIMHRFNEEERKPFIKRMHDKGLIASISVGVKDYEY
                     DFVTSLKEDAPEFITIDIAHGHSNSVIEMIQHIKQELPETFVIAGNVGTPEAVRELEN
                     AGADATKVGIGPGKVCITKVKTGFGTGGWQLAALRWCSKAARKPIIADGGIRTHGDIA
                     KSIRFGASMVMIGSLFAGHLESPGKLVEVDGQQFKEYYGSASEYQKGEHKNVEGKKIL
                     LPVKGRLEDTLTEMQQDLQSSISYAGGKELDSLRHVDYVIVKNSIWNGDSI"
BASE COUNT         1110 a          662 c          480 g          963 t
ORIGIN      
        1 atcaataact aaaaattaac ttttttgata aataaagaaa agtatcatat taatttttta
       61 ggcaatccat tcaaaatgat atttaaaaca acagctgtca gggttgagat aacaatacca
      121 tttgtcaaaa acatttgtaa agtattagga agactaatga agagatttgt cccattaaaa
      181 cctactcctg ctgcaattga tacggctgca atgataaagt tatgctcatt atgctcaaaa
      241 tcaacctgat taagcatttt catcccttga agtgctacca taccaaataa aactaacata
      301 gcaccaccaa gaactggact tggaatcatt tgagccattg cgccaaattt aggtaacaag
      361 ccaagtataa ctaagaagag agctgtaaaa tatatcggct tacgcgtacg tatcccagat
      421 atctgtacca gtcccacatt ttgagaaaaa ccggtatatg gaaaggtatt aaataagccg
      481 ccaagtaata ctgccaatcc ttctgaacgg taaccattac gaagtctctt actgtctaat
      541 ttatcgtttg taatatctga aagcgcaagg taaacacctg ttgattctac catagaaact
      601 gttgcaataa tacacatcat taaaatagaa gtaaattcaa atctaggagc tccaaaataa
      661 aatggcttcg gaatatgtac aagtggtgct tctgctacca cagaagcatc aactaagccc
      721 ataaatgctg caagaattgt acctgatatg agtccaataa ggattgaaat tgacttcaaa
      781 aagccttttg cgaaaatatt aattaataaa acaacaccaa tcgttaccag tgataaagtt
      841 aaagattgta agctcggttc ttttgcatta tcacccatat ttcccatcgc aactggtatt
      901 aatgtcaaac caattgtagt aataactgat cctgttacaa ttggaggaaa gaaatttgca
      961 actttagaaa aaatgccagc aactaaaacg acatatattc ctgaagcaat taaagctcca
     1021 aacatatagc cggaaccttg ttgtgcacca ataattgata aaggagctac agactgaaag
     1081 gcacacccta atacaactgg aagacccact ccaaagtgct tacttaattg taattgtaat
     1141 aaggtggcaa tcccacacat aaaaatatct gtcgcaataa gataagttag ttgctcagca
     1201 ttataaccaa gagcactagc aatcatgata ggtacaagaa tggatcctgc atacatagct
     1261 aacaggtgtt gcaagcctag aagtgctgct tgcgagttac tttgctcatt aaatttcatt
     1321 aagcatcagc ctccgcaaaa acaactcgac cattttcaaa cgctttaata cgagcaagag
     1381 aagtaactgg aacacctgtt ttttctaaca aatcacgccc atcttggaaa gatttttcaa
     1441 taacgattcc gataccagca accttagctc cagcttgacc aataatttca agtaatcctt
     1501 tagccgcttg accgtttgct aaaaagtcat caatgatgag tacagtatca tcgttagata
     1561 aaaagcgact cacaatagaa acttgactcg taacttgctt tgtaaaagaa tacacttcag
     1621 cagttaagat accttcagtc atagtaatgt tcttagcttt tttagcaaat atcattggta
     1681 cgcccaatgc ttgagctgcg tacactgctg gtgcaattcc agatgcttca atcgtaacaa
     1741 ccttcgtaat gccggcttct ttatatttat cagcaaaaac tttacctatt tcctgcatta
     1801 actcaaaatc tacctgatga gtcaaaaaag aatcaacctt taaaatgttc tcacctaaaa
     1861 catcaccatc tttaagaata cgttcttcca ataacttcat aataacccct tcactcttta
     1921 gaaaataaaa aaataactta cctcaatcag aggacacaaa tctcctctaa tttatcgctt
     1981 cattaacatg aaccaacagg caagctattt attagcttac ttattgttag acattacggt
     2041 gtcttgtaga gacgctaagc caatcctcag cataaataag taaatcatat atttaattgt
     2101 tatgtatgat agataactat tgtaacattt ttaattaaga tttcaagatt taatttaaat
     2161 ggaatcacca ttccaaatag aatttttaac aataacgtaa tcaacgtgtc tcaagctatc
     2221 caattctttt cctcctgcgt aagaaataga actttgcaaa tcttgttgca tttctgtcaa
     2281 agtatcctct agtcgccctt taacaggtaa tagaattttc ttaccttcaa catttttatg
     2341 ttctcccttt tgatattcag aagctgagcc atagtattct ttaaattgct gtccatctac
     2401 ttccacaagt tttccaggac tttcaaggtg accagcaaac agcgaaccaa tcattaccat
     2461 actagcacca aagcggatag attttgcaat atccccatgt gttcgaatac caccatcagc
     2521 aataattggc ttgcgagcag ctttagaaca ccaacgtaag gcagctaatt gccaacctcc
     2581 agtaccaaaa cctgtcttga ctttagtaat acaaacttta ccaggtccaa taccaacttt
     2641 agttgcatct gcacctgcat tttccaattc tctaacagct tcaggcgttc ctacatttcc
     2701 agcaataacg aaagtttctg gaagctcttg tttaatgtgt tggatcatct ctataacact
     2761 gttagaatgg ccatgtgcaa tatcaatagt gataaactca ggagcatctt ctttcagact
     2821 tgtcacgaag tcatattcat agtctttgac cccaaccgaa atcgatgcaa ttaaaccttt
     2881 atcatgcata cgcttgataa aaggttttct ttcttcttca ttaaaacggt gcataatata
     2941 aaaataacct tcacacgcta atgtttcagc tacctcttca tcaataatag tctgcatatt
     3001 agcagggatt actggaagtt taaaggtata gtttccaagt ttgacacttg tgtcagcttg
     3061 tgagcggcta gatataatac acttgtttgg tattaattga atatcttcat aatcaaaaac
     3121 aggaatatcg ttaaacataa tggttctcct aatgagtaaa atgaccttcc tataactttg
     3181 tataacaaag catcgctagg taaaaacata ccttt
//
LOCUS       DATRMA010000117         3207 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.118, whole genome shotgun sequence.
ACCESSION   DATRMA010000117 DATRMA010000000
VERSION     DATRMA010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3207
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.118"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            complement(200..766)
                     /locus_tag="VBC28_001908"
     CDS             complement(200..766)
                     /locus_tag="VBC28_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000596040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEO6815856.1"
                     /translation="MIKRPIHLSHDFLAEVIDKEAITLDATMGNGNDTVFLAKSSKKV
                     YAFDIQEEAIAKTKAKLTEQGISNAELILDGHENLEQYVHTPLRAAIFNLGYLPSADK
                     TVITKPHTTIKAIKNVLDILEVGGRLSLMVYYGHDGGKSEKDAVIAFVEQLPQNNFAT
                     MLYQPLNQVNTPPFLIMVEKLQSYENEV"
     gene            complement(759..1694)
                     /locus_tag="VBC28_001909"
     CDS             complement(759..1694)
                     /locus_tag="VBC28_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747785.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HEO6815857.1"
                     /translation="MKKRYRAINDYYRELFGEKIFKLPIDAGFDCPNRDGTVARGGCT
                     FCTVSGSGDAIVAPEAPIREQFYKEIDFMHRKWPEVNKYLVYFQNFTNTHAKLEIIKE
                     RYEQAINEPGVIGINIGTRPDCLPDETIYYLAELSERMHVTLELGLQTTYEATSALIN
                     RAHSYDLYKKTVKRIRELAPKVEIVSHLINGLPGETHDMMVENVRRCVTDNDIQGIKL
                     HLLHLMTNTRMQRDYHEGRLRLLSQEDYISIICDQLEIIPKHIVIHRITGDAPRHMLI
                     GPMWSLNKWEVLNAIDKEMEKRQSYQGCKAEVKHD"
     gene            complement(1787..2428)
                     /locus_tag="VBC28_001910"
     CDS             complement(1787..2428)
                     /locus_tag="VBC28_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEO6815858.1"
                     /translation="MLSRQNSKLIQAFIAIILFFSLGLVIKYWPDTVISFDQTIQESV
                     RGQLPNLSTRFFKLITVIGNTVSQIAIAIMSVTFCYLKKWYPQARFIAVNAIISGICI
                     LSLKLIFQRVRPTLTHLVFAGGYSFPSGHSMGTFMIFGSIIILLQYYMPKSIWKLLCQ
                     GTLGLLIFLIGLSRIYLGVHFPTDVLAGFILAYGILNLTAYIFLAKDWLKKVK"
     gene            complement(2409..2993)
                     /locus_tag="VBC28_001911"
     CDS             complement(2409..2993)
                     /locus_tag="VBC28_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEO6815859.1"
                     /translation="MTNTRKMVTIAILSALSFVLMMVSFPLIPGAEFLKVDFSILPML
                     VAFILFDLKSSYGVLLLRSLLKVILANRGPETFIGLPMNMVALALFLASFAIFWKNRE
                     SAKDFIKASLFGTVSLTVSMVALNYVFAIPLYAIFANFDIRTFIGVGNYLLTMVIPFN
                     IVEGILISIVFYLTYVACLPILERYKKTNVISSK"
     regulatory      complement(3062..3207)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
BASE COUNT         1083 a          614 c          495 g         1015 t
ORIGIN      
        1 tgactgccag ggtcttgcat ggaaaattac tcctaaataa aaattatttg aatttagctg
       61 atagcgatta actctaccaa ctgttcttct attagataat gctatcatta tatcatataa
      121 tagcactgag cgcatagtcg ataatattgt ttatggcatt ttatagaaat tatcatttta
      181 gctaattgcc taaagatagt tacacctcat tttcatatga ttgtaacttt tctaccataa
      241 ttaaaaaggg aggcgtatta acttgattga gtggttgata aagcattgta gcgaaattat
      301 tctgtggtaa ttgttccacg aatgctatca ccgcatcttt ctcacttttt ccaccatcat
      361 gaccataata aaccataagt gatagacgtc cacctacttc taaaatgtct aaaacgtttt
      421 tgattgcctt gatagttgtg tgtggttttg taataactgt tttatccgct gagggtaagt
      481 atcccagatt aaatatagcc gctcttaaag gtgtatgaac atattgttcc aaattttcat
      541 gcccatccaa gataagttca gcgttagaga tcccttgctc cgtgagcttt gctttcgttt
      601 ttgctattgc ttcttcttga atatcaaatg cataaacttt tttagaactt ttcgctaaaa
      661 aaacagtatc attcccattt cccatagttg catcaagcgt tattgcttct ttatcaataa
      721 cttctgctaa aaaatcatga gaaagatgaa ttggtctttt aatcatgttt tacctcagct
      781 ttacatcctt gatatgactg tcttttctcc atttctttgt caatggcatt aagcacttcc
      841 catttattaa ggctccacat cggtccaatt aacatatgac gtggcgcatc accagtaatt
      901 cggtgaataa caatatgttt tggaataatc tccagttggt cacaaataat cgaaatatag
      961 tcttcttgac ttaacaaacg taaacgacct tcatggtaat ctctctgcat tcttgtatta
     1021 gtcataagat gcagaaggtg taattttata ccctgaatat cattatccgt aacacatcga
     1081 cgaacatttt ccaccatcat atcatgtgtc tcccctggaa ggccatttat taggtgagaa
     1141 acgatttcta ctttaggagc taattctcgt attctcttaa cagttttttt gtaaaggtca
     1201 tatgaatgtg ctctatttat taatgcagaa gttgcttcat aagttgtctg aaggcctaat
     1261 tctaaagtca catgcattct ttcagaaagt tcagcgagat agtatatagt ttcatcaggt
     1321 aagcaatccg gccttgttcc gatgttgatt ccgataactc ctggctcatt aatagcctgt
     1381 tcgtaacgct ctttaattat ctctaactta gcatgggtat tggtaaaatt ttgaaaatag
     1441 actaagtatt tattaacctc aggccacttt ctatgcatga aatcaatttc tttatagaat
     1501 tgttcacgaa taggagcttc tggagcaact atagcatccc ctgaaccaga aactgtgcaa
     1561 aaagtgcacc ctcctctagc aactgttccg tctctgttag gacagtcaaa accagcatct
     1621 ataggtaatt taaatatttt ttctccaaag agttctcgat aataatcatt aatcgcacga
     1681 taacgttttt tcataggata attgtatcac aattttaact aaaataacct cactactaca
     1741 ataaaactaa aaaagattgg aacgtcagtt agtcccaatc ttttatttac ttcactttct
     1801 ttaaccaatc cttggctaaa aagatatacg cagttagatt caaaatacca taagcaagta
     1861 taaaaccagc taaaacatct gtcggaaaat gaacccctag gtaaatacga gataacccaa
     1921 ttaaaaaaat gagcaaaccc aaagtacctt ggcacaacag tttccatata ctcttaggca
     1981 tatagtactg caataaaata ataatactcc caaatatcat aaatgttccc atcgagtgcc
     2041 cactgggaaa cgaatagcca cctgcaaata ctaaatgggt taaagttggt cttactcttt
     2101 gaaaaataag ttttaaagaa agtatacata taccagagat aatagcattt actgcgataa
     2161 atctagcttg aggataccac ttcttaaggt aacagaaagt gacgctcata atcgcaatag
     2221 ctatctggct tacagtatta ccaatcacag tgattaactt gaaaaatctt gtagaaagat
     2281 ttggcaactg tcctctaaca ctttcttgaa tagtttggtc aaatgaaatt acagtgtcgg
     2341 gccaatattt gatgaccaat cctaaactga aaaataagat aatagcaata aatgcttgaa
     2401 taagtttact attttgacga gataacatta gtctttttat atctttctaa tattggcaaa
     2461 caagccacgt aagttagata gaaaacaatc gaaattaaaa ttccctcaac gatattaaat
     2521 ggaataacca ttgttaaaag gtaattgcct acaccaataa atgttctgat atcaaagtta
     2581 gcaaatatag catacaaagg aatcgcaaag acatagttga gagctaccat agatacggtc
     2641 aagctaactg taccaaatag actagcttta ataaaatctt ttgcactctc tctatttttc
     2701 cagaaaatag cgaaacttgc taaaaataaa gctagagcaa ccatattcat cggtaaacca
     2761 ataaacgttt ctggaccacg attagcaagt ataactttta aaagtgatct taataagagt
     2821 acaccataac ttgatttcaa atcaaataaa ataaaagcaa ctaacatcgg aaggattgaa
     2881 aaatcaacct ttaaaaattc tgctcctggt attaatggaa atgaaaccat catcaataca
     2941 aaagataagg cagaaagaat ggcgattgtc accattttac gtgtatttgt cataaaaaaa
     3001 ttcctccaat ttaaataaat tgaaagaagc tccaaaggta agcgtatgta cgcgaaaaaa
     3061 cctttgtctt ctcccatcca gactttactg tcggttgtgg aatctcacca catcagcttt
     3121 cgctcgcgga ctgatgcttc acaactgaca aataagttgg aagcgattac cgccggtcgg
     3181 gaattacacc ctgccctgaa gacacct
//
LOCUS       DATRMA010000118         2933 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.119, whole genome shotgun sequence.
ACCESSION   DATRMA010000118 DATRMA010000000
VERSION     DATRMA010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2933
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.119"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            11..1087
                     /locus_tag="VBC28_001912"
     CDS             11..1087
                     /locus_tag="VBC28_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO6815860.1"
                     /translation="MLGENIYLQRTQIGMTQENLSDYLHLTKTTISKWENNQAKPDID
                     YLILMANLFDISLDDLVGYQKTLSDDQRNQLIKDLKIKANVLSERDFFQEVKELSKQF
                     PNDFKTLLIMINMVLSNLTNLNDSEMKEWSLSTLNKIISKTVVESDLQTAIMFKTVVL
                     FHNLEYDEVINLYQNSPYKLGEELLLANSFFAKVDVYRAKQVLQVEIYQQILLVCEYL
                     LSLSTFEVEENKGDIFERLEHMIEAFQLNSLHPNTVIKCYYSLACHYSKSDDDKALGY
                     LKQCFKSLKQLLQRFELHGDTFFYTIDDWLETIPTGIAPPTSQLQVIERVEDLFQNSQ
                     FKELSGHKEFQQMIQKLNDLKKDY"
     gene            1089..1295
                     /locus_tag="VBC28_001913"
     CDS             1089..1295
                     /locus_tag="VBC28_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001090962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLDc N-terminal domain-containing protein"
                     /protein_id="HEO6815861.1"
                     /translation="MNTEIPNQLLPFLVPLIMLQGALIIISLVKLSKLKMTKHLSKPV
                     WFLVIIFLNIIGPIAFLILEGNNE"
     gene            1292..2194
                     /locus_tag="VBC28_001914"
     CDS             1292..2194
                     /locus_tag="VBC28_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6815862.1"
                     /translation="MKAISFQNVTKSFGPKKILNNVSFDLEENMIYGFVGPNGAGKTT
                     TIKMILGLLKFDTGFITIFGKKVNFGRTDTNQLIGYLPDVPEYYDYMTALEYLDLCSG
                     LARSKHKLSNKELLRSVGLDDNHQKIATYSRGMKQRLGLAQALVHDPKIIICDEPTSA
                     LDPKGRQDILDIISNLRGEKTVIFSTHILSDVEKICDHVLVLTKCGIYSLEELKGKKS
                     EENYSVRILIKVTKSEAKVLSHNYQIEKKDNEYALTLKGSKMDNKADLLAGFYQDLVS
                     LKISPSAIEVIDNSLEELYLEVTK"
     gene            2191..>2933
                     /locus_tag="VBC28_001915"
     CDS             2191..>2933
                     /locus_tag="VBC28_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000838141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6815863.1"
                     /translation="MKSLAVMLKKEWMENVRTYKVISILITCSIFGILGPLTALMMPD
                     IMAGILPKKLQGAIPEPTYIDSYIQYFKNMNQLGLVILVFLFSSTLTQEFSKGTLINL
                     VTKGLAKKVIILAKFIVITLLWTVSYLLSVVIHFSYTLYYFSNEGSHKLMVYGATWFI
                     GILFISLILFFSVLFRKTLGGLLGCLFMVVLCVVSGFFKISKSYNPMLLIQKQGEILS
                     GKFKVQDLMEPSILVFLLIIMTLLLSMYI"
BASE COUNT         1020 a          418 c          508 g          987 t
ORIGIN      
        1 agattgaccg atgcttggag agaatatcta tttacaacgt actcagattg gtatgacgca
       61 agagaatttg agtgattatc tgcatttaac aaaaactaca atttcaaagt gggaaaataa
      121 tcaagctaaa ccggatattg actatcttat tttgatggct aatctttttg atattagctt
      181 ggatgactta gtcggttatc aaaaaacatt gtcagatgat caaagaaatc agctcattaa
      241 ggatctaaaa ataaaagcaa atgtattgtc tgagagagat ttctttcagg aagttaagga
      301 attatcaaaa cagttcccta acgattttaa gactctgttg ataatgatta atatggtctt
      361 gagcaaccta accaacctta atgattcaga aatgaaagag tggtcactaa gtacactaaa
      421 taaaattatt agtaaaacag tagttgagtc tgacctccag acggcgataa tgttcaaaac
      481 ggtcgttctt tttcacaacc ttgaatatga tgaagtgata aacctttacc agaatagtcc
      541 ctataaatta ggtgaagaat tacttttagc taatagcttt tttgcaaaag tcgatgttta
      601 ccgagctaag caagtattac aggttgaaat ttatcagcag attctacttg tatgcgagta
      661 tctattgagt ttatcaactt ttgaagttga agaaaacaaa ggcgatattt ttgaaaggct
      721 tgaacatatg atagaagcgt ttcaattaaa ctctttgcat cccaacacag tcatcaagtg
      781 ttattatagt ttagcttgtc attatagtaa aagtgatgat gataaggcat taggatactt
      841 aaaacaatgt tttaaatctt taaaacaatt gctccaaaga tttgaattac acggtgatac
      901 tttcttttat accatcgatg attggttaga gacaatacct actggtattg caccaccgac
      961 gagtcaatta caggtaatag aacgagttga ggacttattt caaaattcac aatttaaaga
     1021 actatcaggg cataaagaat ttcaacaaat gattcaaaaa cttaacgatt taaaaaagga
     1081 ttactaatat gaatacagaa ataccaaatc aattattacc attcctagtt ccgttgatta
     1141 tgttgcaagg agctttaatt atcatttctc tagtcaaact aagtaaactt aagatgacta
     1201 aacacctttc aaagccagtt tggtttttgg tcattatatt tcttaatata attgggccaa
     1261 ttgcatttct tattttggaa gggaataatg aatgaaagct atctcatttc aaaacgttac
     1321 aaaatctttt ggtcctaaaa aaatattaaa taatgtttct tttgatttag aagaaaatat
     1381 gatttatgga ttcgtaggac caaatggagc aggtaaaaca acgacaataa aaatgatttt
     1441 aggattgtta aaatttgata cgggtttcat aacaattttt ggtaaaaaag ttaattttgg
     1501 aagaacagat accaatcaac tgattggcta tctcccagat gttcccgagt actacgatta
     1561 tatgacagct ctagaatatc ttgatttatg ttctggttta gcgagatcaa agcataagct
     1621 ttctaacaaa gaactactta ggtcagttgg acttgacgat aatcaccaaa agattgctac
     1681 ttattctcga ggaatgaaac agcgtcttgg attagcccaa gcactggttc atgatcctaa
     1741 aattattatt tgcgatgaac caacctctgc attagacccg aaaggtcgcc aagatatttt
     1801 agatattatt tcaaatctta gaggagaaaa aactgtgatt ttttctaccc atattctctc
     1861 tgatgtcgaa aaaatatgtg atcatgttct ggtgttaact aagtgtggga tttattctct
     1921 tgaagagtta aaaggaaaaa aatcagagga aaattattcg gttcgtattt taataaaagt
     1981 gacaaagtct gaagctaagg tactatctca taactatcag atagaaaaga aagataatga
     2041 gtatgcactg actctcaaag gctctaaaat ggataacaaa gcagatttac tagctggttt
     2101 ttaccaagat ttagtttctt taaagattag cccatcagct attgaagtca ttgacaatag
     2161 tttagaggag ctttatttgg aggttaccaa atgaaatctt tagcagtaat gttaaaaaaa
     2221 gaatggatgg agaatgttag gacatacaaa gttatatcta ttctgattac ttgttcaata
     2281 tttggtatat taggtccctt aactgcactt atgatgcctg atattatggc aggaatttta
     2341 cctaaaaaac tacagggagc gataccagag cctacctata ttgattctta tattcagtat
     2401 tttaaaaata tgaatcagct tggtttagtt attttagtat ttttatttag tagtacactg
     2461 acgcaagaat tttcaaaagg aaccttaatt aatctagtta cgaagggatt agccaaaaaa
     2521 gttataatcc ttgctaaatt tatagtaatt acccttctat ggacagtgtc atacttatta
     2581 agtgttgtca tccattttag ttatacgtta tattacttta gtaacgaagg aagtcataaa
     2641 ttaatggttt acggtgcaac atggtttatc ggtattcttt tcataagctt aatactcttt
     2701 ttttcagttc tttttcgtaa aactttaggg ggtttgttag gttgtctttt tatggtggtg
     2761 ttatgtgtag tatctggatt ttttaaaatc agtaaaagct ataatcctat gcttcttatc
     2821 caaaaacaag gagaaatatt aagtggaaag tttaaagttc aagacttaat ggaaccgagc
     2881 atattagtgt ttttgcttat tataatgacg ttattactta gcatgtacat ttt
//
LOCUS       DATRMA010000119         2856 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.120, whole genome shotgun sequence.
ACCESSION   DATRMA010000119 DATRMA010000000
VERSION     DATRMA010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2856
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.120"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            complement(<1..1102)
                     /locus_tag="VBC28_001916"
     CDS             complement(<1..1102)
                     /locus_tag="VBC28_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001106850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HEO6815864.1"
                     /translation="MNYKEIYQEWLENDSLGKDIKSDLEAIKGDESEIQDRFYKTLEF
                     GTAGLRGKLGAGTNRMNTYMVGKAAQALANTIIDHGPEAIARGIAVSYDVRYQSKEFA
                     ELTCSIMAANGIKSYIYKGIRPTPMCSYAIRALGCVSGVMITASHNPQAYNGYKAYWK
                     EGSQILDDIADQIANHMDAITDYQQIKQIPFEEALASGSASYIDESIEEAYKKEVLGL
                     TINDTNIDKSVRVVYTPLNGVGNLPVREVLRRRGFENVYVVPEQEMSDPDFTTVGYPN
                     PEVPKAFAYSESLGKSVDADILLATDPDCDRVALEVKDSKGEYIFLNGNKIGALLSYY
                     IFSQRCALGNLPHHPVLVKSIVTGDLSKVIADK"
     gene            complement(1389..2123)
                     /locus_tag="VBC28_001917"
     CDS             complement(1389..2123)
                     /locus_tag="VBC28_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019805208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEO6815865.1"
                     /translation="MVEPIISIQGLHKSFGKNEVLKGIDLDIHQGEVVVIIGPSGSGK
                     STFLRTMNLLEVPTKGTVTFEGIDITDKKNDIFKMREKMGMVFQQFNLFPNMTVLENI
                     TLSPIKTKGLSNLDAQTKAYELLEKVGLKEKANTYPASLSGGQQQRIAIARGLAMNPD
                     VLLFDEPTSALDPEMVGEVLTVMQDLAKSGMTMVIVTHEMGFAREVADRVIFMDAGII
                     VEQGAPKEVFEQTKEIRTRDFLSKVL"
     gene            complement(2116..2808)
                     /locus_tag="VBC28_001918"
     CDS             complement(2116..2808)
                     /locus_tag="VBC28_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001011649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEO6815866.1"
                     /translation="MNFSFLPQYWSYFNYGVMVTIMISTCVVFFGTIIGVLIALVKRT
                     NLHFLTILANFYVWVFRGTPMVVQIMIAFAWMHFNNLPTISFGVLDLDFTRLLPGIII
                     ISLNSGAYISEIVRAGIEAVPSGQIEAAYSLGIRPKNTLRYVILPQAFKNILPALGNE
                     FITIIKDSALLQTIGVMELWNGAQSVVTATYSPVAPLLFAAFYYLMLTTILSALLKQM
                     EKYLGKGVKIDG"
BASE COUNT          958 a          579 c          418 g          901 t
ORIGIN      
        1 atttatctgc aataactttt gatagatcac cagttacaat ggattttacc aatacaggat
       61 gatgtggcaa attccctaag gcacatcgtt gtgaaaaaat ataataggaa agaagtgccc
      121 ctatcttatt accatttaag aaaatatatt ctcccttact atctttgact tccaatgcta
      181 ctcggtcaca atctggatct gtggcaagta agatatctgc atcaactgac tttcctagag
      241 attctgaata ggcaaatgct ttaggaactt cagggtttgg atagccaacc gttgtaaaat
      301 caggatcgga catttcctgc tcaggtacca cataaacatt ttcaaaacca cggcgtctta
      361 aaacttcgcg cacaggtaaa tttcctacgc catttaaagg agtataaact acccggactg
      421 acttatcaat attagtatca ttaatagtta aaccaagcac ttctttttta tatgcttctt
      481 caatactctc atcgatataa cttgccgaac cacttgccag agcctcttca aagggtattt
      541 gcttaatttg ctgataatcg gttatagcat ccatatgatt ggcaatttga tcagcaatat
      601 catctaaaat ctgagatcct tctttccaat atgccttata accattataa gcttgaggat
      661 tatgactagc agtaatcatc acacccgaaa cacatcctag agcacgaata gcatatgagc
      721 acattggtgt tgggcgaatc cctttataaa tataagactt aataccattt gctgccataa
      781 tggaacaagt taattctgca aattccttag attgataacg gacatcataa ctaactgcaa
      841 ttccacgtgc aatagcttca gggccatgat caataatcgt attagctaat gcttgtgctg
      901 ctttccccac catataagta ttcatacgat tggttcctgc tccaagttta cctctcaatc
      961 ccgccgttcc aaattctaat gttttgtaaa aacgatcctg aatttcagat tcatcgcctt
     1021 taatagcttc taaatctgac ttaatatctt taccgagtga gtcgttttct aaccactctt
     1081 gataaatctc tttataattc atatgagaca tcctattcta agaccaaaat ggtaaaaatc
     1141 agctccatga taagagattt tagttcttca ggagctaact gaatagtatc aaattaacct
     1201 tataaagtca acttatttta taaattaact acacttaata tatttaaagc atctaaattt
     1261 ttttcatcac cctatcattc ttagtctaca ttatatatag acttaccaac ttccaaacaa
     1321 tacagtaaca atttactcat aacaatggaa taataaaagt ctaaaacaag ttgttttaga
     1381 caatgaaatt ataatacttt acttaagaaa tctcttgtgc ggatttcttt tgtctgctca
     1441 aatacttcct taggggcccc ttgctcaaca ataattcctg catccataaa aatgacacga
     1501 tccgctactt cacgtgcaaa acccatttca tgagtgacaa taaccatcgt cataccagat
     1561 ttagctaaat cttgcataac agtcaagact tcacctacca tttcaggatc aagagctgaa
     1621 gtaggttcat caaaaagaag gacatcagga ttcattgcaa gacctcttgc aatagcaatt
     1681 cgttgttgtt gtcctccaga taagctagct ggataagtat tagccttctc tttgagtcca
     1741 actttttcaa gtagctcata tgcttttgtc tgagcatcaa gattagaaag cccctttgtc
     1801 ttaataggtg ataaagtaat attttctagt acagtcatat tgggaaatag attgaactgt
     1861 tgaaaaacca tgcccatttt ttcgcgcatt ttaaaaatat cattcttttt gtctgttata
     1921 tcaatccctt caaaagtcac tgttcccttt gttggtactt ccaagagatt cattgttctt
     1981 aaaaatgttg acttaccaga gccagaaggg ccaataataa ccactacttc tccttgatga
     2041 atatccaagt caatgccttt taaaacctca tttttcccaa aacttttatg aagtccttgt
     2101 attgaaataa ttggctcaac catctatttt tacccctttc ccaagatatt tctccatttg
     2161 ttttaacaaa gctgagagaa tcgttgtcaa cattaaatag taaaatgctg caaataataa
     2221 cggtgcaact ggtgagtaag tagccgttac aactgattgt gctccgttcc ataattccat
     2281 gacaccaata gtttgaagga gagcactatc tttaataatt gtaataaatt cattccctag
     2341 agcaggtaaa atatttttaa aagcttgggg taagataaca tagcgaagtg tatttttagg
     2401 tcgaatcccc aacgagtaag ctgcttctat ttgtccagat ggtacagcct caatccctgc
     2461 acgtacaatt tccgaaatat aggcaccact atttaaggaa atgataatga taccaggtaa
     2521 aagtcgtgta aaatctaaat ctaaaacacc aaagctaatc gttggtaaat tgttaaaatg
     2581 catccatgcg aaagcaatca taatttgaac taccatcggt gtcccacgaa atacccatac
     2641 atagaaatta gctaatattg tgagaaaatg taaattagta cgctttacta aagcaattaa
     2701 cacacctata atagttccaa aaaaaacaac acatgttgaa atcataatgg ttaccatcac
     2761 accataatta aaataggacc aatattgtgg taaaaatgaa aaattcattg acatctccta
     2821 aaaaagtaat tgccaatagt ctatcacaca aagaat
//
LOCUS       DATRMA010000120         2850 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.121, whole genome shotgun sequence.
ACCESSION   DATRMA010000120 DATRMA010000000
VERSION     DATRMA010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2850
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.121"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            <1..325
                     /locus_tag="VBC28_001919"
     CDS             <1..325
                     /locus_tag="VBC28_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cation-efflux pump"
                     /protein_id="HEO6815867.1"
                     /translation="KNEKIILAKIPGYETFISPDFYMINEKGNIITSDMLTNATNHSL
                     ASNFKYFNVKSISQKTKLVSYELEGKRHTSIWRRNEKWFLIYHQITAKSSPYKTRRYQ
                     ITSLD"
     gene            484..876
                     /locus_tag="VBC28_001920"
     CDS             484..876
                     /locus_tag="VBC28_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HEO6815868.1"
                     /translation="MKLYVQLMVILTFSFAGEVLSTIFNLPVPGSIIGLILLFLALKY
                     KIIRLRHIDAVGNFLLANMTILFLPPAVGLMEHFQDIKPYLFGIAIIILGALFLNILT
                     IGLVSQWIKKRYEGDYPEIGGKNGNFNE"
     gene            857..1552
                     /locus_tag="VBC28_001921"
     CDS             857..1552
                     /locus_tag="VBC28_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HEO6815869.1"
                     /translation="MATLTNNPIFGIMLTVWAYYIGIRIFRKYPSPATTPLLLATILL
                     IAFLKLTHISYKDYYNGGSFLTMLITPSTVVLAIPLYRTFHLMKHHIKSISISIILAS
                     VINTVFTAIVAKFFGMKYFLAISLFPKSVTTAMAVGITSKAGGLATITLVVVVITGIL
                     TSVLGPIFLKLLRIEDPVAIGLALGGTGHAIGTGQALKYGQVQGAMAGLAIGITGICY
                     VIVSPLVAGLILK"
     gene            1621..2244
                     /locus_tag="VBC28_001922"
     CDS             1621..2244
                     /locus_tag="VBC28_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000612522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3862 domain-containing protein"
                     /protein_id="HEO6815870.1"
                     /translation="MIPLKKITVGTIVCLSFLGLTACSSSNTQQTSTSKSNVSQHKNI
                     KADHEELRLKFNKVKLGVKANNFKGGTSLAELKQLFGGEPNEKFDTPAGNVTLKGYRW
                     NVDDISITIQLLNDSSIVRSISNFKFIRDANITTKDYNSLKNGMSYNKVKELLGEPDD
                     ISQAVSSDKEELQAAWISGIQSSDSDPGINLTFENDKLTNKQQHGLK"
     gene            complement(2646..2792)
                     /locus_tag="VBC28_001923"
     CDS             complement(2646..2792)
                     /locus_tag="VBC28_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6815871.1"
                     /translation="MKKYIAIEIENTKEFEELYDDYLKKASELREAVQKLEQFQFTGI
                     LNNN"
BASE COUNT          977 a          476 c          452 g          945 t
ORIGIN      
        1 caaaaacgaa aaaatcatcc tagcaaaaat tcccggttat gagaccttta ttagccctga
       61 cttttatatg attaatgaaa agggaaacat tatcacttct gacatgctca ctaatgctac
      121 aaatcattca ttggcaagca actttaaata tttcaatgta aaatcaatta gtcaaaaaac
      181 aaaacttgtg tcttatgaat tggaaggtaa acgacatact agtatttggc gccgtaatga
      241 aaaatggttc ttaatctacc atcaaataac tgccaagtca agtccgtata aaacgagacg
      301 atatcaaatc acttcccttg attaatatac tatttaatca gttactcttg ataaaaatag
      361 cttacaaaaa taaattaaaa agattgagaa aaatctcggt ctttttaatt tgtttttatg
      421 taaacgtttc atagccttga aaattacttt cagagtagta gactataatg gaaggagttt
      481 ttcatgaaat tatacgttca attaatggtt attttaacct tttcattcgc tggtgaggtt
      541 ctatctacta tattcaactt acctgtacca ggtagtatta ttggattaat attactattc
      601 ctagcgttaa aatacaaaat catcaggctc aggcatattg atgccgtggg gaattttcta
      661 ttagcaaata tgaccatttt atttttacca ccagcagttg gactgatgga gcactttcaa
      721 gacataaaac cctatctttt tgggatagct atcattatct taggagcgct atttctcaat
      781 attctaacta ttggactggt atcccaatgg atcaaaaaga gatatgaagg agattatcca
      841 gaaattggag gtaaaaatgg caactttaac gaataaccct atctttggta ttatgttaac
      901 cgtatgggcg tattacattg gcataaggat ttttagaaaa tatccttccc ctgcaacaac
      961 tccacttctt ttggcgacta ttttattaat tgctttttta aaattaacac atatctccta
     1021 taaagattac tacaatggcg gttcatttct aacgatgtta attacgcctt caacggttgt
     1081 attggctatt cctctctata gaacctttca tttaatgaag catcacatta aaagtattag
     1141 cattagcatc atcttagctt ctgttatcaa cacagttttt accgcaatag ttgctaagtt
     1201 ttttggtatg aaatatttct tagcaatctc acttttccca aagtccgtaa ctacagctat
     1261 ggccgttgga attacaagta aagcaggtgg attagctact atcactctgg tcgtagttgt
     1321 tattacaggc atcttaacaa gcgttttggg tcctattttt ctaaaattat taaggataga
     1381 agatcctgtt gctatagggc tcgcgctagg aggaactggc catgctatag gaactggaca
     1441 agccttaaaa tatggccaag ttcaaggcgc catggctgga ctagcaattg gtatcacagg
     1501 aatttgctat gttattgtca gtcctttagt tgcaggctta atattgaaat gaggaaaaga
     1561 aaagtaaacc caaaataatt atgttataat atttaaaaat agaatttagg ataaataatt
     1621 atgataccgt taaaaaaaat aactgttgga acgattgtct gtctctcatt tttgggttta
     1681 acagcatgct cttcttcaaa tactcagcaa acctctactt caaaatcaaa tgtatcacaa
     1741 cataaaaata tcaaagctga ccatgaagaa ttacgtttga aatttaataa agtaaagtta
     1801 ggagttaaag caaataactt taaaggaggc actagtctcg ctgaattaaa acaattattt
     1861 ggtggcgaac caaatgaaaa atttgataca ccagcgggaa atgtcactct taaaggctat
     1921 cgttggaatg tcgatgatat ctccattact atacaattat taaacgatag tagcattgtt
     1981 cgttcaatta gtaattttaa gtttattcgt gatgccaaca tcacaacaaa agattataat
     2041 agtttaaaaa atggcatgtc atacaataaa gtaaaagaac ttctaggaga acctgatgat
     2101 atttctcaag cagtttcttc agataaggag gaactacagg ctgcatggat tagtggcatc
     2161 cagtcgtcag acagtgatcc gggaattaac cttacatttg aaaatgataa actcactaac
     2221 aaacaacagc atggtttaaa ataaaaaggc attaacgcct ttttattaat accttaatta
     2281 cttattagaa atattttatt acacaatagt ttaatctcag taccttaact tagtccttta
     2341 atggtcatac taactaatta tgatactaac ttacacagat ttcaaactaa ttcttcctta
     2401 tttgtcaacc ttggttttac ttatcagaaa tgaggagaat cagtttgtga ggcaagattg
     2461 gagataaact aatataaagc ctatagcata cagctttatg gagcatctat tatccacctt
     2521 aagggaaaac acccagattt taagacaaac aattttgctt acaacgttca tgacttgacg
     2581 cttaataagc acttcagttg ctatcaattt tcagaaaacg attcatttaa gttaatagct
     2641 attgattaat tgttgtttaa aattccagta aattggaatt gttcaagctt ttgaactgct
     2701 tctcgtagtt cagaagcttt ttttaaataa tcgtcataaa gttcttcaaa ctcctttgtg
     2761 ttttcaatct caattgcaat gtatttcttc acgaatgcca tcccctttct gtggtataat
     2821 ttaaatggaa atatggagat aaaaatgtat
//
LOCUS       DATRMA010000121         2803 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.122, whole genome shotgun sequence.
ACCESSION   DATRMA010000121 DATRMA010000000
VERSION     DATRMA010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2803
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.122"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            24..1379
                     /gene="rlmD"
                     /locus_tag="VBC28_001924"
     CDS             24..1379
                     /gene="rlmD"
                     /locus_tag="VBC28_001924"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEO6815872.1"
                     /translation="MNVVLKQRIPLKIKRMGINGEGIGFYKKTLIFVPGALKGEEVFC
                     QISSVRRNFAEAKLLKINKKSKNRVEPPCSIYKECGGCQIMHLQYDKQLEFKTDVIRQ
                     ALMKFKPEGYENYEIRKTIGMSEPEHYRAKLQFQVRSFGGNVKAGLYAQGTHRLIDIK
                     DCLVQDSLTQEMINRVAELLGKYKLPIYNERKIAGVRTVMIRRAQASGEVQLIFITSK
                     RLDFDDVVIELVREFPELKTVAVNINASKTSDIYGQITEVIWGQESINEEVLDYGFSL
                     SPRAFYQLNPKQTQILYSEAVKALDVKEDDDLIDAYCGVGTIGLAFAGKVKSVRGMDI
                     IPEAIQDAKENALYMGFTNTHYEAGKAEDIIPRWYSEGFRANALIVDPPRTGLDDKLL
                     NTILKMPPEKMVYVSCNTSTLARDLVTLTKVYHVHYIQSVDMFPHTARTEAVVKLQRK
                     E"
     gene            1648..2218
                     /locus_tag="VBC28_001925"
                     /pseudo
     CDS             1648..2218
                     /locus_tag="VBC28_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771410.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
     gene            2252..2680
                     /locus_tag="VBC28_001926"
     CDS             2252..2680
                     /locus_tag="VBC28_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO6815873.1"
                     /translation="MWNVKTFDNLTTHELFQIYKLRVSVFVVEQDCPYQEVDDEDLIC
                     LHGMNWVDGQLAAYYRLIPEDDKVHLGRVIVNPDFRKKGLGNQLVEYAIKFSEANYPN
                     KPIYAQAQAYLQDFYQSFGFQPVSDIYLEDNIPHLDMVLL"
BASE COUNT          975 a          366 c          557 g          905 t
ORIGIN      
        1 cttagttagg gagtaatgaa ctgatgaatg tggttttaaa acagcgaatt cctttaaaaa
       61 ttaaacgaat gggaattaat ggtgaaggaa taggttttta taaaaaaaca ctaatctttg
      121 ttccaggggc cctcaaggga gaagaggtgt tttgtcagat ttcttctgtt agacgtaatt
      181 ttgcagaagc taaactgtta aagataaata aaaaatctaa aaatagggta gaaccccctt
      241 gttctattta taaagaatgt gggggatgcc aaataatgca ccttcaatat gataagcagc
      301 tagagtttaa aacggatgtt attcgacagg ctctaatgaa atttaaacca gagggatatg
      361 agaattatga aatccgtaaa acgataggta tgtcggaacc tgaacactat cgtgctaaat
      421 tacaatttca agtgcgttcg tttggaggga atgtaaaggc aggtttatat gcacaaggga
      481 cacatcgatt gattgatatc aaagattgtt tagtgcaaga tagccttact caggaaatga
      541 taaatcgagt tgcagaactt ttaggaaaat ataaattgcc tatttataat gaacgtaaaa
      601 tagctggtgt ccgtactgtt atgattcgac gagcacaagc gagtggagag gttcaactga
      661 tctttatcac aagtaaaaga cttgattttg atgatgttgt tattgaatta gttcgagagt
      721 ttcctgagtt aaagacagtt gctgtaaata tcaacgctag taaaacaagt gatatctatg
      781 gtcaaataac agaagttatt tggggtcaag aatccatcaa tgaagaagta ttagattatg
      841 gtttttcgtt atcaccacgt gctttttatc aacttaatcc taaacaaaca caaattttat
      901 atagtgaggc agttaaggcc ttagatgtga aggaagatga tgatttaatt gacgcttatt
      961 gtggagtagg aacgattgga cttgcttttg ctggaaaagt taaaagtgtt cgtggaatgg
     1021 atattatccc tgaggctatt caggatgcta aagagaacgc actttatatg ggttttacta
     1081 atacacatta cgaagctgga aaggcagagg atattatccc taggtggtat agtgagggat
     1141 ttcgcgctaa tgcgttaatc gtggatcctc ctcgcacagg tttagatgat aaattgctaa
     1201 atactatact aaaaatgcct ccagaaaaaa tggtttacgt ttcttgtaat acatccacgc
     1261 ttgctagaga cttagtaaca ttaacaaaag tataccatgt ccactacatc caatccgttg
     1321 acatgtttcc acatacagca aggaccgagg ctgtagtgaa actacaacgt aaggaataat
     1381 aaagttttct aatcaaaaaa taagagtatt tttgggtatt cttattttta gatagtaatg
     1441 gtgagatatt aatataacat agtctaaaaa cacatggttt aagctatttg atacggaaat
     1501 ttaccttaga aatggtaaat agttccatta agtatattca tctttgcaaa tttgaccttg
     1561 ccttttaagt ttcattgagt taaaatagaa aaaatatttt taacaagatg atagaacatc
     1621 gtaaattaag gtttaaagga aatgcttatg ataataaatt cattcgataa tagtagaaaa
     1681 gcgattatta atcctgaaga tattaattca ccaataaaag gttttccaaa gactgttatt
     1741 acctgtttcg cgcgggagac tttcaatcgt attttagaag aattgcctca tcgtgaaatt
     1801 gctagaacta gtgtagcaaa cttagaaatt cccatttatg aattagaatt taaaggacaa
     1861 aagattggct ttttcaatgc ttatgttggt gcttcagctt gcgtagcaat cttggaagat
     1921 attatagtat ttggaatgga aagtttaatt gtttttggaa catgtggcgt ccttgattcg
     1981 tcaattgagg agacatccat tattattcca agaagtgcta ttagggatga agggacatca
     2041 tttcattatt cggaagcaag ttctgaaatt gcagtaaaca caaattcaat ttttttactc
     2101 tgcggataat ttagatgcag aagtatggga agcaagatct ttaggaaata gcgaggattt
     2161 agaaaagaaa gataaaattg cttatattgc catggagttt gctatagaga ctttttaggg
     2221 tctactgtat gatgttgaaa ggaagatagc aatgtggaat gtaaaaactt ttgataattt
     2281 aactacacat gagttgtttc aaatatataa attaagagta tctgtatttg tggttgaaca
     2341 agattgtcct tatcaagagg ttgatgatga agatttgatt tgtttgcatg ggatgaattg
     2401 ggtagatggt caattggcag cttattatag attgatacca gaagatgata aggtgcattt
     2461 gggacgtgtg attgttaatc cagactttag aaaaaaggga ttaggaaatc aattagtgga
     2521 atatgcgatt aaatttagtg aggcaaatta ccctaataaa cctatttatg ctcaggccca
     2581 agcttattta caagattttt atcaatcttt cggatttcaa cctgtctcag atatctatct
     2641 agaagataat attcctcatc ttgatatggt tttattataa atacatgcta tttaaactat
     2701 caattaaaag gtctctcagt attgctgaga gatcttttaa ttaacaaaaa attaaaaaat
     2761 ttaaattaaa aaataaaaat aaaatttatc gctaattatt cta
//
LOCUS       DATRMA010000122         2614 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.123, whole genome shotgun sequence.
ACCESSION   DATRMA010000122 DATRMA010000000
VERSION     DATRMA010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2614
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.123"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            61..495
                     /locus_tag="VBC28_001927"
     CDS             61..495
                     /locus_tag="VBC28_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6815874.1"
                     /translation="MKKLIKYILSFENIIAIFFIVLFGLLLIRIKPVNNSVYSIISGI
                     AIDKVIIVIISSFVIAIFSAFFIIDILEIIFHLKIDSLFLIAYLFFAISLGFGLLKVL
                     TESQFSLLSAFIAFPLFLIFPKLFEKIVNNRKINQKDNKNQK"
     gene            888..1676
                     /locus_tag="VBC28_001928"
     CDS             888..1676
                     /locus_tag="VBC28_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008087996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO6815875.1"
                     /translation="MARGRGKLTPQEIEALRFFSSKLNDLLNETSTKQVELSKATGIP
                     PSTLTGYVKGTSLPIPGNVQKIADYFGLMKSDIDPRFAPRKKKHELKIPTSPLVNKIT
                     KTVVELNVPRKQQVLDYATEQLKEQKNKITSIEETLYEYKVYEKLSAGTGYGYFGDGN
                     YDTVFYDEQLDYDFASWVFGDSMEPTYLNGEVALIKQTGFDYDGAIYAVEWDGQTYIK
                     KVFREKGGLRLVSLNKKYKDKFAPFSEDPRIIGKVVGNFMPKEY"
     gene            1685..1936
                     /locus_tag="VBC28_001929"
     CDS             1685..1936
                     /locus_tag="VBC28_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6815876.1"
                     /translation="MGLFNKNEKASKKSMVDIYLEQHGINDFPEEYKNAIKNIVNDLS
                     SHGLAKASMILSFSKIEEQAKVGYLSTIINQNWILMNQN"
     gene            2326..2614
                     /locus_tag="VBC28_001930"
                     /pseudo
     CDS             2326..2614
                     /locus_tag="VBC28_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657652.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
BASE COUNT          947 a          381 c          404 g          882 t
ORIGIN      
        1 acccaaacaa catttttttg atgttcatat ttttcccttt ctatgtgata taatcatatt
       61 atgaaaaaat taattaaata tattcttagc ttcgaaaata taattgccat attttttatt
      121 gttctttttg gactattgct cattcgtatt aaacctgtta ataattctgt ttattctata
      181 atctctggta ttgctatcga caaagttatc attgtgataa tttcatcttt tgttattgct
      241 attttctcag catttttcat tatcgatata ctggaaatta tttttcatct caaaattgat
      301 tctttatttt taattgctta tttatttttt gcaatttcac taggttttgg acttcttaaa
      361 gttttgacag aatcacaatt tagcttgtta tctgccttta ttgctttccc tttatttctt
      421 atctttccta aactttttga gaaaatagta aataacagga aaatcaatca aaaagacaat
      481 aaaaatcaaa aataaataaa ccaaaaactc tttaataaca atctcctctc tttcttgaaa
      541 gaaagtaacc actatgatat agtaagcata acccctttaa ggggcgagga ttttcaatcc
      601 cctatccgat taccacgtaa tcagatattt tattttgagc ctaaaccaaa gaatttgaat
      661 ttcgaacttt acttctttgt gtttaggttt tttatttcgc ctactttcca tcagtggcta
      721 cctcctttcg ttttgcttaa ttcctcaagc ttggttatat tgtaatatga tttaaatcgt
      781 attttaatca ttttttcggt aaaaatcgta tttattccaa aaatattatt tacaaatacg
      841 atttaaaacg gtattatata agtataaaaa ttcgaaagga aaaaattatg gcaagaggaa
      901 gaggtaaatt aactcctcaa gaaatagagg cattaagatt cttttcgagc aaactaaatg
      961 atttgctcaa tgaaacaagt acaaagcaag tcgaactctc aaaggctaca ggaataccac
     1021 caagcactct tacaggttat gttaaaggaa catctttacc tattccaggt aacgtccaaa
     1081 aaatagcaga ttactttgga ttaatgaagt ctgatataga cccaagattt gcacctcgta
     1141 agaaaaaaca tgaactcaaa attcctactt ctccgctcgt taacaaaatt actaagacgg
     1201 ttgttgaatt aaatgttcca cgtaaacaac aagtactaga ttatgctaca gagcaactaa
     1261 aagagcaaaa aaataaaata acctccatag aagaaacgtt gtacgaatat aaagtatatg
     1321 aaaaactctc tgctggtaca gggtatggtt actttggcga cggaaattac gacactgtct
     1381 tttatgatga gcaattagac tatgattttg cttcttgggt tttcggtgat tcaatggaac
     1441 cgacttatct taatggcgaa gtcgcactaa taaagcagac aggctttgat tatgatggag
     1501 ctatatatgc tgtcgaatgg gatggacaaa cttatattaa gaaagtattt agagaaaagg
     1561 gtggtttaag attagtttct ttaaacaaaa aatacaaaga taagtttgca ccttttagtg
     1621 aagatccacg tatcatcgga aaagttgttg gcaattttat gccaaaagaa tattgaggtg
     1681 ggatatggga ttattcaata aaaatgagaa agcctctaaa aagtcaatgg tcgatatata
     1741 tcttgagcag catggtatca atgactttcc agaggagtat aaaaatgcta ttaaaaatat
     1801 agtaaacgat ctttcgtcac atggactagc aaaagcctct atgattttat ctttttctaa
     1861 aatagaagaa caagcaaaag tgggatatct gtcaacaata attaatcaaa actggattct
     1921 catgaaccaa aactagcaaa tcattaatga attaaaaaaa atgaacgaaa aataaaagaa
     1981 tgcacataaa tctgcttagt acgatttccg tattatcgga gaagttattg gtagttattc
     2041 agaaaatcat agctgatgaa gtgatggtta ttgatgagta ttatagtctg ctggatacag
     2101 tgtaaaaaat acggtagatt tttttccaat acggtagaaa ttcaaagttg tataaaatca
     2161 gataaagtta aataactttt gaaattaata taataatatc agtttagtta gttgacaata
     2221 ataataatat aatataaaat gaagataata gtataagcgc tggtatctct acggggacca
     2281 gtacggagta agccttcttc atttttaatg gaggaggttt tctttttgaa agaatttaaa
     2341 tcacttactg atcaactcaa tttattaaaa gatcgtggtc taaaaatagg agatgaagaa
     2401 aaagctgaat tatatctatt gaccaataac tactacaaca tcataaacgg ttacggaaat
     2461 tcttttgctc tgaaaaaaat aaatacgatg atgatgcaac atttgaaaaa atagctcagt
     2521 tatatttttt tgataaagaa attaagcaaa tactattctc gtcgattttg gatgttgaga
     2581 agcatttgaa atcttcaata gcacatcgat ttgc
//
LOCUS       DATRMA010000123         2444 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.124, whole genome shotgun sequence.
ACCESSION   DATRMA010000123 DATRMA010000000
VERSION     DATRMA010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2444
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.124"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            28..489
                     /locus_tag="VBC28_001931"
     CDS             28..489
                     /locus_tag="VBC28_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO6815877.1"
                     /translation="MERYVKENIERLLYTSGLTQNELADKANLSRQTVNKLLSSNEKY
                     SPKLSTLIAISNVLLINFPELLIRSNKLSASINFTLNSVEEYQNTLIQNVKLYLRSWK
                     QFSLSNSSGVRESTISNLVNGKYSDVYFSTIESLAKEMEVPLADLFKRGES"
     gene            489..1709
                     /locus_tag="VBC28_001932"
     CDS             489..1709
                     /locus_tag="VBC28_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEO6815878.1"
                     /translation="MQFIPYDDELEVINNLHKYNSNDYTVIRLTQTMLNKSIIDANGF
                     LRTLLLDNDLLDFRNLTDKVYLTANLVLDNQKHRVKISFYKANKRGDERFWIYGLGKF
                     IKSSQINVNDLIYITVNNQKELTLFNVTHSIPQNSTIIELFGQDKVEDSLNRLIPQIK
                     AIARQGFHNNSKGAGKIAPKDAGDTLESLLGIQTNNSQEADFEGIIELKSKSSKTLDT
                     LFTLRPNFEGTPVAEFEPNDRSRVSAFARLYGYDSDKHLGYKSLYITIGSEENPQNNQ
                     GFYLEVNDSDNIVELRKQENKKSILTAFWTFEALEEELHKKHPATLWVKVESKMDGEV
                     GQFKYVEAELTRSPQFMTFLSLIKSGAITYDWRGYTTPAGKYVGKNHGNAWRIKNKQR
                     GLLFGSTEVIDLSF"
     gene            1726..>2444
                     /locus_tag="VBC28_001933"
     CDS             1726..>2444
                     /locus_tag="VBC28_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014619950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4297 domain-containing protein"
                     /protein_id="HEO6815879.1"
                     /translation="MAKDNGGAIAQKGFNYQNCVVSLVAIRNYKKPNFSIYVEADEDF
                     EVMYDDDYHAYIQVKGQKGMSIKKLLSTTNNKPSIFEKNLSSGTDDSKYKIVVYNFNE
                     KDLKEMQEQIDTEELFYSSWLLSDSQKNKVSNPKTKNFSLVKTDFDNNISAARTFLKG
                     ELANQKISIDNRDDVILDELLQQIIQKSEKEIHSDADKELKKITSEELNLILQKITAK
                     ARFDKELKKFGFTSIKNEKIKR"
BASE COUNT          958 a          331 c          439 g          716 t
ORIGIN      
        1 taaaaacaat agttggagaa aatagatatg gaacgctatg ttaaagaaaa tattgaacgg
       61 ttactttaca caagcggatt aacgcaaaat gagttagctg ataaggcgaa tcttagcagg
      121 caaactgtta ataaactatt atcttctaat gaaaagtata gtcctaaact ttcaacttta
      181 atagcaattt ctaatgtctt attaataaat tttccagagc tccttattag aagcaataaa
      241 ttaagtgctt ctataaactt tactctaaat tctgtagaag aatatcaaaa tacacttata
      301 cagaatgtga aattatattt gagaagttgg aaacaatttt cactaagtaa ttccagtgga
      361 gtaagagaat ctacgataag taatcttgta aatggtaaat actcagacgt atatttttca
      421 acaatagaaa gccttgctaa ggaaatggaa gttccgcttg ctgatttatt taagagggga
      481 gaaagctaat gcaatttatt ccatatgatg atgagttaga ggttatcaat aatctacata
      541 agtataattc taatgactac actgttatca gattgacaca aactatgcta aataaaagta
      601 ttattgatgc aaatggattt ctaagaactt tgctattaga taatgacttg ttagatttta
      661 ggaacttgac tgataaagtt tatttgacag ctaatcttgt actggacaac caaaagcata
      721 gggtaaaaat aagtttctat aaagctaaca aaagaggaga tgaaagattt tggatatatg
      781 gattagggaa gttcattaag tcatctcaaa tcaatgtaaa cgacttgatt tatataaccg
      841 taaataatca aaaagaactt acattattta atgttacaca tagcattcca caaaatagta
      901 ctattataga actatttgga caagataaag ttgaagattc actaaataga ttaattccac
      961 agattaaagc catagcaaga caaggctttc ataataattc taagggggca ggtaagattg
     1021 caccgaaaga cgctggagat accctagaat cacttttggg aattcaaacc aataatagcc
     1081 aagaagctga ttttgaagga atcattgagc taaaatcaaa atcctctaaa accctagata
     1141 ccctatttac tttacgtcca aatttcgaag gaacgcccgt agcagaattt gaaccgaatg
     1201 ataggagtag agtgtcggcg tttgctcgtc tgtatggcta tgactcagat aagcatttgg
     1261 gatataagag cttatacatt acaataggtt cagaagaaaa tcctcaaaac aatcaaggtt
     1321 tctatctgga agttaatgat agcgataata ttgttgagct acggaaacag gaaaacaaaa
     1381 agagtatttt aactgccttt tggacatttg aagctctaga ggaagaatta cataaaaaac
     1441 acccagccac cttatgggtg aaagttgaat ctaaaatgga cggcgaagta ggtcaattca
     1501 agtacgttga agcagaatta actcgttcac cacaatttat gacttttctt tctctgataa
     1561 aatcaggagc aattacttat gattggcgag gctatactac accagcagga aagtatgtag
     1621 gtaagaatca cggtaacgct tggcggataa aaaataagca aagaggtttg ttatttggta
     1681 gtacagaagt aattgattta agtttttagt atttggagaa attttatggc aaaagataat
     1741 ggtggggcaa ttgcacaaaa gggattcaat tatcaaaatt gtgttgtgtc cttagttgca
     1801 attagaaatt acaaaaaacc taatttttca atatatgtag aggcagacga agattttgaa
     1861 gttatgtacg atgatgatta tcatgcctat attcaggtta aaggacaaaa aggcatgagt
     1921 attaaaaaat tattgagtac cactaataat aaaccgtcaa tctttgaaaa aaatctatcg
     1981 tctggtacag atgactctaa gtataagatt gtggtgtata attttaatga aaaagacttg
     2041 aaggaaatgc aggagcaaat agatacagag gaacttttct atagctcatg gctattatct
     2101 gatagtcaga aaaataaagt tagcaatcct aaaacaaaaa acttttcgtt agtgaaaact
     2161 gattttgata acaatatcag cgctgctagg acatttttaa aaggggaact agcgaatcag
     2221 aaaatttcta ttgataatag agatgatgta atccttgatg aattattaca gcaaattatc
     2281 caaaaatcag aaaaagaaat acactcagat gctgataaag aacttaaaaa gattacatca
     2341 gaagaattaa atctaatttt acaaaaaata actgcaaaag caaggtttga taaagagtta
     2401 aaaaaatttg gatttacatc tataaaaaac gaaaagatta aacg
//
LOCUS       DATRMA010000124         2354 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.125, whole genome shotgun sequence.
ACCESSION   DATRMA010000124 DATRMA010000000
VERSION     DATRMA010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2354
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.125"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            complement(<1..531)
                     /locus_tag="VBC28_001934"
     CDS             complement(<1..531)
                     /locus_tag="VBC28_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HEO6815880.1"
                     /translation="MFHTKSLIQMAMMATLLVVLGFIPALPLGFIPVPIVLQNLGVMM
                     AGVILGGRKGSLSILLFFLVGLVIPVFSGLRSTIPVLTGPTAGYVIAWLFVPLLISYG
                     VKLVNKKNFIAIFLIVWLAGVLFVDVAGAVWLASYTNIPLDKSLLQNLVFIPGDTIKA
                     IIAAVVAVKYKDSFFLT"
     gene            complement(538..849)
                     /locus_tag="VBC28_001935"
     CDS             complement(538..849)
                     /locus_tag="VBC28_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000983195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6815881.1"
                     /translation="MMQEYFGIGLDSSSRCYHYHTKLDIVALKCAVCQKYYACYKCHD
                     ALEEHCFAATKSDETFPVLCGSCRQMLTLKEYKTGFCPYCRMLFNPNCQRHDSIYFSK
                     E"
     gene            complement(956..2098)
                     /gene="tgt"
                     /locus_tag="VBC28_001936"
     CDS             complement(956..2098)
                     /gene="tgt"
                     /locus_tag="VBC28_001936"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEO6815882.1"
                     /translation="MTDHPIKYRLIKQEKHTGARLGEIITPHGTFPTPMFMPVGTQAT
                     VKTQSPEELKEMGSGIILSNTYHLWLRPGDELIAKAGGLHKFMNWDQAILTDSGGFQV
                     YSLADSRNITEEGVTFKNHLNGAKMFLSPEKAISIQNNLGSDIMMSFDECPQFYQPYD
                     YVKKSIERTSRWAERGLNAHRRPHDQGLFGIVQGAGFEDLRRQSARDLVSMDFPGYSI
                     GGLAVGETHDEMNAVLDFTVPMLPNDKPRYLMGVGAPDSLIDAVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKFAEDFTPLDPNCDCYTCKNYTRAYIRHLLKADETFGIR
                     LTSYHNLYFLVNLMKDVRQAIMDDNLLEFRQDFMERYGYGMNNRNF"
BASE COUNT          811 a          457 c          383 g          703 t
ORIGIN      
        1 agtcaagaaa aaactatctt tatatttaac agcaacaaca gcagctataa tggcttttat
       61 tgtatctcct gggataaata ctaagttttg aagaagtgat ttatctaatg ggatgttcgt
      121 ataagatgcc agccaaactg ctcctgctac atctacaaaa aggacccctg ctagccatac
      181 aataaggaaa atagctataa aattcttttt atttactaat tttacaccat atgagataag
      241 caaaggaaca aacaaccaag caataacata accagctgtt ggtcctgtca atacaggtat
      301 agtcgaacgt aaacctgaga atacaggaat aactaaaccg acaagaaaga agagcaaaat
      361 agataaactc ccttttcttc cgcccaaaat aacaccagcc atcataaccc ctaggttttg
      421 taagacgata gggaccggaa tgaaaccaag tggtagagca ggaatgaaac caagtaccac
      481 caataaagta gccatcatag ccatctggat taggcttttt gtgtgaaaca ttaaaaatta
      541 ctccttcgaa aaataaatag aatcatggcg ttgacaattt ggattaaaaa gcatacgaca
      601 ataaggacaa aaaccagttt tatactcttt taatgtcaac atctgacgac aagaaccgca
      661 taagacagga aacgtttcat cagattttgt tgctgcaaaa caatgctcct cgagtgcatc
      721 atgacattta taacaagcat aatatttttg acaaacagca cattttaaag cgacaatatc
      781 caatttagta tgatagtggt aacagcggct gctactatca agaccaatac caaagtactc
      841 ttgcatcatg attcctccta taacatatta tacaaaagtt tttctattgt caactttttt
      901 aaaaaaacgg ttaacaaaca attcaaaaaa aagccctgtt ttaccaggaa cttttttaaa
      961 aattacggtt attcatgcca tagccatagc gctccataaa atcttgacgg aattctagaa
     1021 gattatcatc cataatagct tgtctgacat ctttcataag attgacaagg aaataaagat
     1081 tatggtaact agtcaaacga ataccaaaag tttcatctgc tttcaataag tgacgaatat
     1141 aagcgcgtgt ataattttta caagtatagc aatcgcaatt tggatcaaga ggcgtaaaat
     1201 cttctgcaaa tttagcattc ttaacaacta agcgaccttg acttgtcata catgtcccat
     1261 ttcgagcaat acgagtaggt aagacgcaat caaacatatc tactcctcgg ataacggcgt
     1321 ctattaaact atctggtgca ccaactccca tcaaataacg aggcttatca ttcggaagca
     1381 ttggaacagt aaaatcaaga actgcattca tctcatcatg tgtttctccg accgcaagcc
     1441 ctccaattga ataacctgga aaatccatcg atactaaatc tcgtgctgat tgacgacgta
     1501 aatcctcaaa ccctgcccct tgaacaatac caaatagtcc ttgatcatga ggacggcggt
     1561 gggcatttaa tccacgttct gcccaacggc ttgttcgctc aattgatttt tttacataat
     1621 cataaggctg gtaaaattgt ggacactcat caaaactcat cataatatca gaaccaaggt
     1681 tattttgaat tgaaatagct ttttctggtg ataaaaacat tttcgcacca tttaaatggt
     1741 ttttaaaggt tactccttct tcagtgatgt ttcgactatc agccaaagaa taaacctgaa
     1801 agcctccact gtctgttaga atagcctgat cccagttcat gaatttatgt aaacctccag
     1861 ctttagcaat caattcatca cctggacgta accataaatg gtaggtattt gatagaataa
     1921 ttcctgaacc catttctttt agttcttcag gagattgtgt cttaacagtt gcttgtgtcc
     1981 caacaggcat aaacattggt gtcggaaatg taccatgtgg tgtgattatt tcaccaagtc
     2041 tggctcctgt gtgtttttcc tgtttaatta atcgatattt tattggatga tcagtcatcg
     2101 ttttctcctt gcttccatga aaaacagtca tgggctttag tgctaatcaa tatctaaatg
     2161 taatactttg tattaatttc ttttttagcc attaagattt tatcaaaaaa atgtactctt
     2221 gtcatgctca tcttaaggaa tgtgttataa tgaaatgaat tgtacttttg tgtatggagg
     2281 agatatgacg aattcagaaa ttaaaaatga ggcaaaaact atattaagca atcttcaagg
     2341 taagaatcaa cttt
//
LOCUS       DATRMA010000125         2322 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.126, whole genome shotgun sequence.
ACCESSION   DATRMA010000125 DATRMA010000000
VERSION     DATRMA010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2322
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.126"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            complement(<1..685)
                     /locus_tag="VBC28_001937"
     CDS             complement(<1..685)
                     /locus_tag="VBC28_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO6815883.1"
                     /translation="MIENYLEKDILNQIKLLTLCYDYYPSITLDKSCHQLGLSELLIR
                     KYCHDLTTLFNSQLSLNIEKSTIVYQSNGVTREQAFKCIYHQSHVLQLLKFLITNDSG
                     RLPLTYFSEKFGLSCATAYRIRKHISPLLEKLGFQIVKNTITGDEYRIRYLIAFLNAQ
                     FGIEVYPMSKMDKLLIKRLLLEHSTTFTASHYFPNTFIFFDTLLSLSWKRINYNVVVP
                     YSSLFTELQN"
     gene            967..>2322
                     /gene="pilA"
                     /locus_tag="VBC28_001938"
     CDS             967..>2322
                     /gene="pilA"
                     /locus_tag="VBC28_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-2a pilus adhesin PilA"
                     /protein_id="HEO6815884.1"
                     /translation="MRKYQKFSKILTLSLFCLSQIPLNTNVLGESTVPENGAKGKLVV
                     KKTDDQNKPLSKATFVLKTTAHPESKIEKVTAELTGEATFDNLIPGDYTLSEETAPEG
                     YKKTNQTWQVKVESNGKTTIQNSGDKNSTIGQNQEELDKQYPPTGIYEDTKESYKLEH
                     VKGSVPNGKSEAKAVNPYSSEGEHIREIPEGTLSKRISEVGDLAHNKYKIELTVSGKT
                     IVKPVDKQKPLDVVFVLDNSNSMNNDGPNFQRHNKAKKAAEALGTAVKDILGANSDNR
                     VALVTYGSDIFDGRSVDVVKGFKEDDKYYGLQTKFTIQTENYSHKQLTNNAEEIIKRI
                     PTEAPKAKWGSTTNGLTPEQQKEYYLSKVGETFTMKAFMEADDILSQVNRNSQKIIVH
                     VTDGVPTRSYAINNFKLGASYESQFEQMKKNGYLNKSNFLLTDKPEDIKGNGESYFLF
                     PL"
BASE COUNT          910 a          313 c          417 g          682 t
ORIGIN      
        1 tattttgtag ttcagtgaaa agggatgagt aagggacaac tacattataa ttaatacgtt
       61 tccatgatag agacaacaat gtatcaaaga aaataaatgt atttgggaag taatgagaag
      121 cagtaaaagt agttgagtgt tctaataaca atcgtttgat gagcaattta tccatcttag
      181 acatgggata aacttctata ccaaattgag catttaaaaa tgcgattaaa tagcgaattc
      241 gatactcgtc accggtaata gtatttttga caatctgaaa tccaagtttt tctagtaatg
      301 gactaatatg ttttcgtatg cgataagcag ttgcacaaga tagtccaaat ttttcactaa
      361 agtaagttaa aggtaacctg cctgaatcat tcgtgatcaa aaattttaaa agttgtaaaa
      421 cgtgtgattg atgataaata catttgaaag cttgttctct agttacacca ttagattggt
      481 agactattgt actcttttcg atattcagag agagttgact gttaaataaa gttgttaaat
      541 catgacaata tttcctaata agtagttcag ataaaccaag ttgatgacat gatttgtcta
      601 gagtaatgga agggtagtaa tcatagcata aagttagaag ttttatttga ttaaggatgt
      661 ctttttctaa ataattttca atcaattcag taaactctct caaacattat ttttaagcaa
      721 tattttaaaa cataaaaaaa gaaaaatcaa ctacttaagc tcattgaaat atttctaaga
      781 taataaaaaa taagattatc aaataaaaag aaaaattatt caaaaattgg gaaaaaacat
      841 taaaattcca taccttataa taagaaatta ttgatatcat aataagtgat agtttgtata
      901 ttctaggata ttctgtatct gatcttagat ttagaaacga catttcggca caataggagt
      961 tgtaaaatga gaaaatacca aaaattttct aaaatattga cgttaagtct tttttgtttg
     1021 tcgcaaatac cgcttaatac caatgtttta ggggaaagta ccgtaccgga aaatggtgct
     1081 aaaggaaagt tagttgttaa aaagacagat gaccagaaca aaccactttc aaaagctacc
     1141 tttgttttaa aaactactgc tcatccagaa agtaaaatag aaaaagtaac tgctgagcta
     1201 acaggtgaag ctacttttga taatctcata cctggagatt atactttatc agaagaaaca
     1261 gcgcccgaag gttataaaaa gactaaccag acttggcaag ttaaggttga gagtaatgga
     1321 aaaactacga tacaaaatag tggtgataaa aattccacaa ttggacaaaa tcaggaagaa
     1381 ctagataagc agtatccccc cacaggaatt tatgaagata caaaggaatc ttataaactt
     1441 gagcatgtta aaggttcagt tccaaatgga aagtcagagg caaaagcagt taacccatat
     1501 tcaagtgaag gtgagcatat aagagaaatt ccagagggaa cattatctaa acgtatttca
     1561 gaagtaggtg atttagctca taataaatat aaaattgagt taactgtcag tggaaaaacc
     1621 atagtaaaac cagtggacaa acaaaagccg ttagatgttg tcttcgtact cgataattct
     1681 aactcaatga ataacgatgg cccaaatttt caaaggcata ataaagccaa gaaagctgcc
     1741 gaagctcttg ggaccgcagt aaaagatatt ttaggagcaa acagtgataa tagggttgca
     1801 ttagttacct atggttcaga tatttttgat ggtaggagtg tagatgtcgt aaaaggattt
     1861 aaagaagatg ataaatatta tggccttcaa actaagttca caattcagac agagaattat
     1921 agtcataaac aattaacaaa taatgctgaa gagattataa aaaggattcc gacagaagct
     1981 cctaaagcta agtggggatc tactaccaat ggattaactc cagagcaaca aaaggagtac
     2041 tatcttagta aagtaggaga aacatttact atgaaagcct tcatggaggc agatgatatt
     2101 ttgagtcaag taaatcgaaa tagtcaaaaa attattgttc atgtaactga tggtgttcct
     2161 acgagatcat atgctattaa taattttaaa ctgggtgcat catatgaaag ccaatttgaa
     2221 caaatgaaaa aaaatggata tctaaataaa agtaattttc tacttactga taagcccgag
     2281 gatataaaag gaaatgggga gagttacttt ttgtttccct ta
//
LOCUS       DATRMA010000126         2313 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.127, whole genome shotgun sequence.
ACCESSION   DATRMA010000126 DATRMA010000000
VERSION     DATRMA010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2313
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.127"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            119..>2313
                     /gene="srr1"
                     /locus_tag="VBC28_001939"
     CDS             119..>2313
                     /gene="srr1"
                     /locus_tag="VBC28_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-rich repeat glycoprotein adhesin Srr1"
                     /protein_id="HEO6815885.1"
                     /translation="MSQKTFGKQLTVVDTKSRVKMHKSEKNWVRTVMSHFNLFKAIKG
                     RATVEADVCIQDVEKEDRLSSGNLTYLKGILAAGALVGGASLTSRVYADETPVVQEQS
                     SSVPTLAEQTEVTVKTTTVQNHQDGTVSKNIIDSNSVSMSESASTSTSESVSMSMSGS
                     TLTSVSESVSTSALTSASESISTSASESVSKSTSISEVSNILETQASLTDKGRESFSA
                     NQIVTESSLVTDAGKNASVSSLIEITKPKSELQTSKMSNESLITPEKSQVMIASDKTG
                     NESLTPTIRLKSVIQPRSMNLMTLSSEMDLIPLEEVSDTEMLGKDVSSELQKVNIALK
                     DNTLSEPGTVKLDSSENLVLNFAFSIASVNEGDVFTVKLSDNLDTQGIGTILKVQDIM
                     DETGQLLATGSYSPLTHNITYTWTRYASTLNNIKARVNMPVWPDQRIISKTTSDKQCF
                     TATLNNQVASIEERVQYNSPSVTEHTNVKTNVRSRIMKLDDERQTETYITQINPEGKE
                     MYFASGLGNLYTIIGSDGTSGSPVNLLNAEVKILKTNSKNLTDSMDQNYDSPEFEDVT
                     SQYSYTNDGSKITIDWKTNSISSTTSYVVLVKIPKQSGVLYSTVSDINQTYGSKYSYG
                     HTNISGDSDANAEIKLLSESASTSASTSASTSASMSASTSASTSASMSASTSASTSAS
                     TSASMSASTSASTSASTSASTSASTSASMSASTSASTSASTSASTSASTS"
BASE COUNT          798 a          401 c          454 g          660 t
ORIGIN      
        1 gtgcttaacg gtatcattat ttttgtgttt aactaagata gatttctaat cacttaattt
       61 tactgttatg tttttaaacc aaaacaaaat ataaaaaata tttatatatg gaggaaacat
      121 gtcccaaaag acttttggca agcagttaac agttgtagat actaagagta gagtcaagat
      181 gcataaatca gaaaaaaact gggtaagaac agtaatgtcg cattttaatc tatttaaagc
      241 gattaaaggg agagcaactg ttgaagcaga tgtgtgtatt caagatgttg aaaaagaaga
      301 ccgactatct tcaggaaatt tgacctatct caaaggaata ctagctgctg gagctctggt
      361 aggtggagcg agtttaacca gtcgtgttta tgcagatgag actccagttg ttcaagaaca
      421 atcaagttct gtaccaacac tggcagaaca aacggaagtg actgttaaaa caactactgt
      481 tcaaaatcat caagatggga cagtatcgaa aaacattatt gattctaata gtgtatctat
      541 gtcagagtca gcctcaacaa gtactagtga atctgtaagt atgtctatgt cagggtcaac
      601 tttaacaagt gtaagtgaat ctgtaagtac atctgcttta acaagtgctt cagaatcgat
      661 aagcacgtca gcctcagaaa gtgtttcaaa atctacaagt attagtgagg tttcaaatat
      721 tcttgaaact caagcttctt taactgataa aggaagagag tcgttttcgg caaaccagat
      781 agtaacagaa agtagcttag ttactgatgc tggtaaaaat gcttcagtat ctagcctaat
      841 tgaaattaca aaaccaaaat cggagttaca gacttccaaa atgtcaaatg agtcgcttat
      901 aactccagag aaatcccaag taatgattgc aagcgataaa actgggaatg agagtctaac
      961 tccgacaatt agattaaaat cagttattca gccaaggagt atgaacttga tgactttgag
     1021 ttcggagatg gacttgatac cactagaaga agtgtctgat actgaaatgt taggtaaaga
     1081 tgtatcaagc gagttgcaga aagttaatat tgcgttaaaa gataacactc ttagtgagcc
     1141 tggaacagtt aaattagata gttcagaaaa ccttgttttg aactttgcct tttcaatcgc
     1201 ttctgttaac gagggagatg tctttactgt aaagctttct gataaccttg acacacaagg
     1261 gattggtact attctaaaag ttcaagatat aatggatgaa acggggcagt tattagcgac
     1321 tgggtcatat agtcctttaa cacataatat tacatacacc tggacaaggt atgcttctac
     1381 gttgaataat attaaagcta gagtcaatat gccagtttgg cctgaccaga gaataatttc
     1441 taaaacaact tcagataagc agtgctttac tgcaacattg aacaatcaag ttgcttcaat
     1501 tgaggaacgt gttcagtata atagtccttc agtgacagaa catactaatg ttaagacaaa
     1561 tgtaagatct cggatcatga agcttgatga tgaaagacag acagaaactt atattactca
     1621 aattaatcct gaaggtaagg aaatgtattt cgcatcagga cttgggaatc tatatactat
     1681 tatcggttca gatggaacat caggttcacc agttaattta ttaaatgcgg aagtaaagat
     1741 tctaaaaact aattcaaaaa atcttacaga tagtatggat caaaattatg attcgcctga
     1801 gtttgaagat gtgacttccc agtatagtta tactaacgat ggttctaaaa ttaccataga
     1861 ttggaaaaca aattctattt cttccactac atcttatgtt gttttggtca aaatacctaa
     1921 acaaagtggt gtattgtatt caactgtttc tgatataaat caaacatatg gttctaaata
     1981 ttcttatggg catacgaata taagtggtga ctcagatgcg aatgccgaaa ttaaactttt
     2041 atcagaaagt gcttctacga gtgcgtcgac gtcagcaagt accagcgctt ccatgagtgc
     2101 ctcgacatca gcaagtacca gcgcttccat gagtgcgtcg acgtcagcca gcaccagtgc
     2161 ttcaacaagt gcaagtatga gtgcttcaac aagtgcaagt accagcgcct caaccagtgc
     2221 cagtaccagt gcttccacct cagcaagtat gagcgcctca acaagtgcaa gtactagcgc
     2281 ttccacaagt gccagtacca gtgcatccac gtc
//
LOCUS       DATRMA010000127         2303 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.128, whole genome shotgun sequence.
ACCESSION   DATRMA010000127 DATRMA010000000
VERSION     DATRMA010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2303
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.128"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            complement(<1..378)
                     /locus_tag="VBC28_001940"
     CDS             complement(<1..378)
                     /locus_tag="VBC28_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000794358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEO6815886.1"
                     /translation="MKNNIIFSIEQYINDQEMPKGKQKVILSAIELFASQGFHGTSTA
                     QLAKNAEVSQATIYKYFETKDKLLVFILELIVQTIGRPFFTELSTFSTKEELIHFFVQ
                     DRFKFIEKNNDLIKILMQELLINS"
     gene            complement(402..1499)
                     /locus_tag="VBC28_001941"
     CDS             complement(402..1499)
                     /locus_tag="VBC28_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6815887.1"
                     /translation="MRIIAITEKVIKELFRDKRTLAMMFLAPILIMFLMNVMFSANSN
                     TKVKIGTINVNTKVVSNLDNIKHIQVRSFKFNSSAKKALKSNKIDALISEDNKSYTVF
                     YANTDSSKTTLTRQAFKTAVNTMNSKELISQVKILANKNPKLAQSLQTRSKYIKEKYN
                     YGNKNTGFFAKMIPILMGFMVFFFVFLISGMALLKERTSGTLDRLLATPVKRSDIVFG
                     YMLSYGILAIIQTIVIVLSTIWLLDIQVVGSIFSVIIVNFILALVALSLGILMSTLAK
                     SEFQMMQFIPLIIMPQLFFSGIIPLENMASWAQTVGKILPLSYSGDALTKIIMYGQGL
                     PNVSSNLLVLLLFLIILTIANIFGLKRYRKV"
     gene            complement(1502..2218)
                     /locus_tag="VBC28_001942"
     CDS             complement(1502..2218)
                     /locus_tag="VBC28_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6815888.1"
                     /translation="MKKVIDLKKLQKAYASETVLNNINLEVFKGEIIGLIGPSGAGKS
                     TLIKTMLGMEKADKGTALVLDTQMPDRNILNQIGYMAQSDALYESLTGLENLLFFGKM
                     KGIQKTELKQQITHISKVVDLENQLDKFVSGYSGGMKRRLSLAIALLGNPTVLILDEP
                     TVGIDPSLRRKIWQELINIKDEGHSIFITTHVMDEAELTSKVALLLRGNIIAFDTPLH
                     LKKQFNVSTIEEVFLKAEGE"
BASE COUNT          752 a          357 c          370 g          824 t
ORIGIN      
        1 actattgatt aataattctt gcatcaatat tttaatgaga tcattatttt tttcaataaa
       61 tttaaaacgg tcctgtacaa agaaatgaat tagttcctct ttagtagaaa atgtagataa
      121 ttctgtaaag aaggggcgtc caatagtttg tacgattaac tcgagaataa agaccaaaag
      181 cttgtcttta gtttcaaaat atttatatat agttgcttgg ctaacttctg catttttagc
      241 tagttgagca gtggaagttc catggaagcc ttgtgaggca aagagctcta tagcggataa
      301 aataactttt tgttttcctt ttggcatctc ttggtcatta atatactgtt caatagaaaa
      361 aataatatta tttttcatac ttagttcctt ttcaaatggt attatacttt acggtatcgt
      421 tttagtccaa agatatttgc gatggttaaa ataattaaaa atagaagaag taccagaaga
      481 tttgaagaaa cgttaggtaa tccttgaccg tacataatta tttttgtaag tgcatcacca
      541 gagtaggaga gcggtagaat tttaccgact gtctgagccc aacttgccat attttctaga
      601 gggattattc ctgaaaagaa taattgaggc attataatta agggaataaa ttgcatcatt
      661 tgaaattctg attttgctag agtagacatt aagattccta atgataaggc aaccagagcc
      721 aaaataaaat tgacaatgat gacactaaaa atactaccta caacttgaat atctagtagc
      781 caaatagttg ataaaacgat aactatcgtt tgaataattg ctagaatgcc gtatgataac
      841 atatatccaa atacaatatc actacgttta acaggagttg caaggagtcg atcaagtgta
      901 ccagaagtac gctcttttaa taaagccatt ccagaaatta aaaaaacaaa gaagaagacc
      961 ataaatccca ttagtattgg tatcattttt gcaaaaaagc ctgtattttt atttccgtaa
     1021 ttatattttt ctttgatata tttggagcga gtttgtaagg attgtgctag tttcggattc
     1081 ttattagcta aaattttaac ttgcgaaatc agttccttac tgttcattgt attaacagcg
     1141 gttttaaaag cttgtcttgt taaagtcgtc tttgaagaat ctgtattcgc atagaagaca
     1201 gtataagatt tattgtcctc cgaaataaga gcatcaattt tatttgattt gagtgctttt
     1261 ttagcagatg agttaaattt aaatgatctc acttgaatat gcttaatatt atctaaattt
     1321 gaaacgacct tcgtgttaac gttaatagtt ccaatcttaa cttttgtatt actattcgca
     1381 gaaaacataa cattcatcaa aaacataatt aaaataggtg ctaaaaacat catagcaagt
     1441 gttcttttat cacgaaacag ttcttttata accttttcag ttattgcaat aattctcatg
     1501 attattctcc ttcagctttt aagaaaactt cctcaatagt actcacatta aattgttttt
     1561 ttaaatgtaa tggagtatca aaggcaataa tgtttccacg taatagtagt gcaaccttac
     1621 ttgttaattc tgcttcatcc ataacgtggg ttgtaataaa gatagaatgt ccttcatcct
     1681 taatattaat tagctcttgc cagatttttc tcctcaagga tggatcaatt ccaacggtag
     1741 gttcatctag gattaaaact gtggggtttc caagtagggc gatggctaga gaaagccgtc
     1801 ttttcatacc tcctgagtaa cctgagacaa atttatcaag ttggttttct agatctacta
     1861 ctttagaaat atgagttatc tgctgtttta attcagtttt ttgaatacct ttcatttttc
     1921 caaagaataa taaattttct aagccagtta aagactcgta taaggcatca gattgagcca
     1981 tatagccaat ttgatttaaa atattacgat ctggcatttg agtatcaaga acaagagctg
     2041 ttcccttatc tgctttttcc atgccaagca tagttttaat caaggtagat ttccctgctc
     2101 cagagggtcc tattaatcca attatttcgc ctttaaacac ctccaaatta atattattta
     2161 aaacggtttc tgaggcatat gctttttgta gtttttttaa atcgatgact tttttcatga
     2221 taactttttg ctcctctctc agtgagtgag tgtttactca cttatttgat atcatatatt
     2281 atagtgtgta aagacaggct tgt
//
LOCUS       DATRMA010000128         2127 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.129, whole genome shotgun sequence.
ACCESSION   DATRMA010000128 DATRMA010000000
VERSION     DATRMA010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2127
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.129"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            <1..139
                     /locus_tag="VBC28_001943"
     CDS             <1..139
                     /locus_tag="VBC28_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Rgg/GadR/MutR family transcriptional regulator"
                     /protein_id="HEO6815889.1"
                     /translation="LGDTSSGKEDMRKALQIFKYLGEDSFYNTYNEHYCQKVLGNNKE
                     C"
     gene            288..1109
                     /gene="mutM"
                     /locus_tag="VBC28_001944"
     CDS             288..1109
                     /gene="mutM"
                     /locus_tag="VBC28_001944"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HEO6815890.1"
                     /translation="MPELPEVETVRKGLERLVVNQEIASITIKVPKMVKTDLNDFMIS
                     LPGKTIQQVLRRGKYLLFDFGEMVMVSHLRMEGKYLLFPNKVPDNKHFHLYFKLTNGS
                     TLVYQDVRKFGTFELVRKSSLKDYFTQKKLGPEPTADTFQFEPFSKGLANSKKPIKPL
                     LLDQRLVAGLGNIYVDEVLWAAKIHPQRLANQLTESETSLLHKEIIRILTLGIEKGGS
                     TIRTYKNALGEDGTMQKYLQVYGKTGQPCPRCGCLIKKIKVGGRGTHYCPRCQCL"
     gene            1100..1693
                     /locus_tag="VBC28_001945"
     CDS             1100..1693
                     /locus_tag="VBC28_001945"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017770961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEO6815891.1"
                     /translation="MLMTKIIGLTGGIASGKSTVTKIIRESGFKVIDADQVVHKLQAK
                     GGKLYQALLEWLGPEILDADGELDRPKLSQMIFANPDNMKTSARLQNSIIRQELACQR
                     DQLKQTEEIFFMDIPLLIEEKYIKWFDEIWLVFVDKEKQLQRLMARNNYSREEAELRL
                     SHQMPLTDKKSFASLIIDNNGDLITLKEQILDALQRL"
     gene            1817..>2127
                     /locus_tag="VBC28_001946"
     CDS             1817..>2127
                     /locus_tag="VBC28_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO6815892.1"
                     /translation="MELVIRDIRKRFQETEVLRGASYRFYSGKITGVLGRNGAGKTTL
                     FNILYGDLAADNGTICLLKDNHEYPLTDKDIGIVYSENYLPEFLTGYEFVKFYMDLHP
                     S"
BASE COUNT          759 a          303 c          420 g          645 t
ORIGIN      
        1 acttggtgat acttcttcag gaaaagaaga tatgaggaaa gctttacaga tttttaaata
       61 tttaggtgaa gattcatttt acaatactta taatgaacat tattgtcaaa aagtacttgg
      121 caataataaa gaatgctaat gagtgcaagt ttatgagtaa tttttaaagc ttttgtctca
      181 aaatgataat aagaaaggaa aatagaaatc gaatggtcag gtgacatcaa gaacgtttgg
      241 aaattattgc tctaagcaat gactattgat ttttagacta tatatttatg ccagaattac
      301 cagaagtaga aaccgttaga aaaggactag aaagacttgt agttaatcaa gaaatagctt
      361 ctataacgat taaagtccct aaaatggtga aaactgattt gaatgacttt atgataagtc
      421 taccaggaaa aacaattcaa caggtattga gacgagggaa gtatcttctt tttgattttg
      481 gtgaaatggt tatggtatca catttacgca tggaaggaaa atatctactc ttcccaaata
      541 aagtaccaga taacaaacat tttcaccttt atttcaaatt gactaatggc agcacattgg
      601 tttaccaaga tgtccgtaaa tttgggacat ttgagttggt tcgtaaatca tctctcaaag
      661 attattttac tcaaaaaaaa ttaggtcctg agccaactgc tgacacattt caatttgagc
      721 ctttttcaaa aggcttagca aactccaaaa agccaataaa acctttacta ttagatcaga
      781 gattagtagc aggacttgga aatatttacg tagatgaagt cttgtgggca gcaaaaattc
      841 acccacagcg tttggctaat caattgaccg aatctgaaac aagcttatta cacaaggaaa
      901 ttattcgcat tttaacttta ggcatcgaaa aaggtggctc cacgattaga acatataaga
      961 atgctcttgg agaggacggt acgatgcaaa agtatttaca ggtctatggt aaaactgggc
     1021 aaccgtgtcc aaggtgtggt tgtttaatta agaagataaa agtaggaggg agagggacgc
     1081 attattgccc tcggtgtcaa tgcttatgac aaaaataata ggactgacag gagggatagc
     1141 ttctggaaag tcaacggtaa caaaaataat acgagaatca ggttttaaag tcatagatgc
     1201 ggatcaagtg gttcataaat tgcaagctaa gggtgggaaa ctttaccaag ctttattaga
     1261 atggttgggt cccgagatac ttgatgctga tggtgagttg gatagaccaa agctttctca
     1321 aatgattttt gctaatccag acaatatgaa gacatcagct aggctacaaa atagtatcat
     1381 tcgtcaagag ttagcatgtc agcgcgacca attaaaacaa acagaagaga tatttttcat
     1441 ggatattcct ttattgattg aagaaaagta tataaaatgg tttgatgaga tttggttggt
     1501 atttgttgat aaagaaaaac aattacaacg attaatggcc cgtaacaact acagtcgaga
     1561 agaagcagaa ttacgacttt cacaccaaat gcctttaaca gataaaaaaa gtttcgctag
     1621 tcttattatt gacaataatg gtgatttaat aactttaaaa gagcaaatat tggatgctct
     1681 tcaacgttta tagaactact aaatggataa tgtatttatc cattttttgt ctttattaca
     1741 ctagttttaa aaaacttgcg attaaaccac agtaatagta caatgatata aaaggtttac
     1801 tttcaaagga gtttttatgg agttagtaat tagagatatt cgtaagcggt ttcaggaaac
     1861 agaggtcttg agaggagcaa gttaccgatt ttattcaggt aaaataacag gggtcttagg
     1921 taggaatggt gctgggaaaa caactttatt taatatactt tatggggatc ttgcagctga
     1981 caacgggacc atttgtttat tgaaggataa tcacgagtat cctcttaccg ataaggatat
     2041 tggtattgtt tattccgaaa actaccttcc agaattttta acagggtatg aatttgtaaa
     2101 attttacatg gatttacatc cttcaga
//
LOCUS       DATRMA010000129         2044 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.130, whole genome shotgun sequence.
ACCESSION   DATRMA010000129 DATRMA010000000
VERSION     DATRMA010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2044
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.130"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            29..1933
                     /locus_tag="VBC28_001947"
     CDS             29..1933
                     /locus_tag="VBC28_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003134160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEO6815893.1"
                     /translation="MKSLITALKEKRALRLYFIGIIIFIIGLLLPISSTVRVLISIIA
                     TILSGYHVMLEGIDDTIKETVKKKRFTPNTHILMTLAAIGAIFLGEAVEAALLIFIFA
                     GAHFLEEYVEGKSKREITKLIELNPTEARLINNEGNIEVVSIDKLKIGDRLQVLNGAQ
                     VPTDGIILNGSTSIDESTINGESIPREKNVGDIVFGSTINGTGTFTMRVSKDSKDTVF
                     SKILTMVEESQNNLSPTADFIKRFEPHYVNGVLVIFLALIIFSPMFLGWTLTETISRS
                     LVFLVSASPCALAVAAIPATLAGISSLAKQGILFKGGNFLSNLINLKAVAFDKTGTLT
                     EGKPKVVDFYFNPELDGESFRNTLVAMEKQSNHPLANAIVNKFEIQKVDYDIKVENEL
                     GTGLSATYQGESYRIAKPSAFKIVSQEWIEKQENFENEGKTVVFIAVNDKVEGLIAIQ
                     DTPQKTAISAIQYLNKNQIKTIMITGDAVLTGKAIGKQLGVRDVQANVMPDEKAKIIN
                     ENKEKYGPTAMIGDGVNDAPALAVADVGIAMGDGTDVAIETADVVLMNNDLGNFVSAH
                     KTSKRLKNIVTQNIIFALLVVLFLVYISLWKNTSVVLGITLHEGSTLVVLLNSLRLLL
                     PNKKDIEGEL"
BASE COUNT          768 a          280 c          388 g          608 t
ORIGIN      
        1 tttatatcat ttagaagagg tatctaatat gaaaagctta attacagctt taaaagaaaa
       61 gagagcatta cgcttatatt ttatcggaat aataattttt atcatcggat tattactccc
      121 aatatcaagt acagtcagag ttttaattag cataatagca actattttat cgggatatca
      181 tgtaatgctt gaagggatag atgataccat aaaagagaca gtcaaaaaga aaagatttac
      241 tcctaatacg catattttaa tgacacttgc cgcaataggt gcaattttct taggagaagc
      301 cgttgaagct gctctactaa tctttatatt cgcaggggca cactttcttg aagaatatgt
      361 tgagggaaaa agtaaacggg aaattactaa actaatagaa ctaaatccta cggaagctcg
      421 tttaattaat aatgagggaa atatagaagt agtttcgatt gataaattaa aaataggtga
      481 tagacttcaa gttttgaatg gagcgcaagt tccaactgat ggtatcatac ttaatggttc
      541 aacaagcatt gatgagtcaa ccataaatgg tgaaagtatt cctcgagaaa aaaatgttgg
      601 tgatattgtt tttggttcaa ccattaatgg tacaggtaca ttcactatga gagtatccaa
      661 ggatagcaag gatacagtat tttctaaaat attaactatg gttgaagagt cacaaaataa
      721 cctttcacca actgccgatt tcataaaacg ttttgaacca cactatgtca acggagtatt
      781 agtaatattt ttagctttaa ttatattttc tccaatgttt ttaggctgga cattaacgga
      841 aacaatttca agaagtttag tctttctagt ctctgcttca ccatgtgcat tagctgttgc
      901 tgcaattcct gcaacattag ctggaatatc atctttagca aaacaaggaa tactatttaa
      961 aggtggtaat tttctttcaa atttaattaa tttaaaagct gtcgcttttg ataaaacagg
     1021 aacattgaca gaaggaaaac caaaagtagt agatttctat tttaatcccg aacttgatgg
     1081 tgaatccttt agaaatacat tagtagcaat ggaaaagcaa agtaatcacc cattagcaaa
     1141 tgctatagtt aataaatttg aaattcaaaa agttgactac gatatcaaag tagaaaatga
     1201 acttgggaca gggttgagcg caacctacca aggagaaagc tatcgaattg ctaaaccaag
     1261 tgcttttaaa atcgtaagtc aagaatggat tgaaaaacag gaaaactttg aaaatgaagg
     1321 aaaaacggtg gtatttattg ctgttaacga taaagtcgaa ggcttaatag caattcaaga
     1381 tactcctcaa aagactgcaa tttcggcaat tcaatattta aataagaatc aaataaagac
     1441 tattatgata actggtgatg cagtattaac gggaaaggca attgggaaac agttaggcgt
     1501 aagagatgtg caggcaaatg taatgcctga tgaaaaggca aaaataataa atgaaaataa
     1561 agaaaaatat ggtccaaccg cgatgatcgg agatggtgtg aatgatgcac cagcacttgc
     1621 tgtagcagat gtaggtattg cgatgggaga tggcacggat gtagctatag aaactgcaga
     1681 tgtagtatta atgaataatg accttggtaa ttttgtgagt gcacataaaa cttctaaaag
     1741 attaaaaaat attgtaactc aaaatataat atttgcttta ttagttgttc tatttttagt
     1801 gtatattagt ctatggaaaa atacctcggt ggtactaggc ataacattgc atgagggaag
     1861 tacgctggtt gttctgctaa attcattgag attattattg ccaaataaaa aagatataga
     1921 aggagagcta taaatgttta aaaatatatt tcaaaactta actgaacaag cacaagaaat
     1981 tttggggaat ataaccgaaa atattcctga acaaattcaa aatgttgcag gaggtataac
     2041 cgaa
//
LOCUS       DATRMA010000130         1986 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.132, whole genome shotgun sequence.
ACCESSION   DATRMA010000130 DATRMA010000000
VERSION     DATRMA010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1986
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.132"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            77..673
                     /locus_tag="VBC28_001948"
     CDS             77..673
                     /locus_tag="VBC28_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001874207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO6815894.1"
                     /translation="MNPDHFEDLFQVYGELSTEDSLTYISFSKFNSKNEFDVFFQTLL
                     KSEDPYYLAIVDNNTGKVLGTFSLMRIDTKNRVVEMGWVVYSSKLKQTRIATEAQYLV
                     MKYVFEELCYRRYEWKCDSLNAPSNNSAKRLGFTFEGTFRQAVVYKGRNRDTNWYSIL
                     DKEWPEKKTRFEKWLDDSNFAVNGYQIRSLSSIEQKTK"
     gene            complement(933..1880)
                     /locus_tag="VBC28_001949"
     CDS             complement(933..1880)
                     /locus_tag="VBC28_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor regulator N-terminal
                     domain-containing protein"
                     /protein_id="HEO6815895.1"
                     /translation="MIKTKFSDPLEQLAKKRKRKTLFKTVVLALITVLLVVGIAFKGF
                     TYLTSKNGQKTYHNFELLSEIAYPNISYDSLYYQPSGQFTGKVHADRFKDIDGVQIPY
                     SSFEENYSISGTFGTNADEKSEKNGLYDRGTRQKVPQFFNKNVKYKKGEAKTTPTNDL
                     KYVNKLNNDLIEIAITFDKSYSYKEIKTMIPNNIKQNWLWIGTSTELDTSYWPTKYQF
                     GTDPETLNTPQIFIDKIKENLKGNSKVYFNNINIYSDLEKYAKKYDKVNNSDKLKFSG
                     IILTGKAENFKQLKEKEWVHASSIGANIEYKPYYKLDKE"
     gene            complement(1877..>1986)
                     /locus_tag="VBC28_001950"
     CDS             complement(1877..>1986)
                     /locus_tag="VBC28_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000025252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-70"
                     /protein_id="HEO6815896.1"
                     /translation="EISEITGQSQSTIKIRLMRARREVKKYLEKDNFKI"
BASE COUNT          630 a          274 c          297 g          785 t
ORIGIN      
        1 tgtcgttgaa aattttacgg aaggtaaaaa tccgaagatt gatacactta atgggaaaac
       61 agttagaata gaaaaaataa atccagatca ttttgaagac ttatttcaag tttatggaga
      121 attgtctact gaagatagtt taacttatat atcttttagc aaatttaata gtaagaatga
      181 atttgatgtc tttttccaaa ctctattaaa atcagaagat ccttattatt tagctattgt
      241 tgataataat actggtaaag tattagggac tttttcatta atgcgtattg atacaaaaaa
      301 tagagttgtt gaaatgggat gggtggttta ttcatcaaaa ctaaaacaaa caaggatagc
      361 aacagaagct caatatttgg ttatgaaata tgtttttgaa gaactctgtt accgccgtta
      421 tgagtggaaa tgtgatagtt tgaatgcacc atctaataat tctgcaaaac gacttggttt
      481 tacttttgaa ggaactttta gacaagctgt cgtatataag ggtcgtaaca gagatacaaa
      541 ttggtattct attttagata aagagtggcc agagaaaaaa acacggtttg aaaaatggct
      601 agatgattca aactttgctg taaatgggta tcaaatcaga tcactatcaa gcattgagca
      661 gaagacaaaa tagagtcatt cattaatcac taaaatttca aaattacatg tttttttagc
      721 taacatctta aactttgtta aataaatgtt tattaacttt taaaggtgca tggtaggact
      781 caaaatcggg tgtcctcacg gacgtgccag ctcgactttg gtcgcgggca tcaaagaaaa
      841 aagtcaagat tattatcttg tctttttttc tatgagttat taactgtaaa agttacataa
      901 aattaccaaa taaatttttt aacaattaat tatcattctt tatctaattt ataatagggc
      961 ttatattcta tgtttgcccc tatacttgaa gcatgtaccc attctttttc tttaagttgt
     1021 ttaaagtttt cggctttacc tgttaatatt attcccgaaa attttaattt gtcgctatta
     1081 tttaccttgt cgtatttttt ggcatacttt tctaaatctg agtagatatt tatattatta
     1141 aagtatactt ttgagttacc ttttaagttt tcttttattt tatctatgaa tatttgtggt
     1201 gtattcaaag tttcaggatc tgtaccgaat tgatactttg ttggccaata tgatgtatct
     1261 aattctgtgc ttgtacctat ccataaccaa ttttgtttta tattatttgg tatcattgtt
     1321 tttatttctt tgtaactata ggacttatca aaagttattg caatttcaat aagatcatta
     1381 ttcaatttat ttacatattt taaatcattt gttggagttg ttttggcttc tcctttttta
     1441 tacttaacat ttttattgaa aaattgtgga actttttgtc tagtacctcg atcatacaag
     1501 ccattctttt ctgatttttc atcagcattt gtgccaaaag tacctgagat actataattt
     1561 tcttcaaaac tagaatatgg aatttgaacc ccatcgatat ctttaaatct atcagcatgt
     1621 actttaccag taaactgacc gcttggctgg taatagagac tatcatagga aatatttgga
     1681 tatgctattt cagaaagtag ttcaaagtta tgatatgttt tttgaccatt ttttgaagtt
     1741 aaatatgtaa atcctttaaa agctattcca actactaata gtacagttat taatgccaat
     1801 actacggtct tgaataatgt tttccttttc ctttttttgg ctaattgttc cagtggatca
     1861 ctaaactttg tttttatcat attttaaaat tatccttttc taaatatttt ttaacttctc
     1921 ttctagctcg cataagtctt attttaattg tactttgtga ttgtccagta atttcagata
     1981 tttctt
//
LOCUS       DATRMA010000131         1957 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.133, whole genome shotgun sequence.
ACCESSION   DATRMA010000131 DATRMA010000000
VERSION     DATRMA010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1957
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.133"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            <1..325
                     /locus_tag="VBC28_001951"
     CDS             <1..325
                     /locus_tag="VBC28_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6815897.1"
                     /translation="GKVDYNSVAVISAATDSSNYKDLIGAVSSAQPHQSSTQLKSADK
                     FLKDVQSHDKWTVTQLSGYSQSAYMLKLGAQYHIPTTVFNGWFRYSTLNEDEKNSWLS
                     ILNIL"
     gene            298..978
                     /locus_tag="VBC28_001952"
     CDS             298..978
                     /locus_tag="VBC28_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6815898.1"
                     /translation="MAKHPEYFVNFRHKEDNVTWWNDFNKLDDKDYGTVKWVNGKSHK
                     IESWKFTDDGKLKDEKGNIVNPKSPAVQSVLYEEVHFQKAKAKLKKSGGKLSHSEKVY
                     LDSEQAIFIANGLTTASQTASDDIKKNAELVKEKASELFAKTKVMPPGITDLSPEELA
                     DTYSEGGVREDTIVTPIETFFDEKVTNAQEITTSYINLQKQIESGVQKLLEEDSKLAG
                     EFKEWSQY"
     gene            963..1349
                     /locus_tag="VBC28_001953"
     CDS             963..1349
                     /locus_tag="VBC28_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6815899.1"
                     /translation="MVTVLDQNENKINSLKINLGKLEDNYHRENMEIEDKLYSLEDKK
                     NELETYLQEVYEETSHLLRQNDSDGSTFMSLNRIIESYQMELIDEYENYKQTIIVQEE
                     NSRTQFLKDRVKLENDISSLEGRKYY"
     gene            1383..1664
                     /locus_tag="VBC28_001954"
     CDS             1383..1664
                     /locus_tag="VBC28_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6815900.1"
                     /translation="MSLIGAVLKGTKEDKEFKKQIQRALVRIVYAPEIVEEADSIKMT
                     YSDKIDNVSETAKSSIKTIANQIDSGLVGQFGEKVKAQVDLNKGSYDDL"
BASE COUNT          783 a          251 c          363 g          560 t
ORIGIN      
        1 gggtaaagta gattataaca gtgttgcagt tatatccgcc gcaacggatt caagtaatta
       61 taaagattta attggcgcag tttcatctgc tcaaccacat caaagttcga ctcagttaaa
      121 atcagctgat aaatttttaa aagacgtaca gtcacatgac aaatggactg tgactcagct
      181 ttcaggctat tctcaatctg catatatgtt aaaattaggt gctcaatatc atatcccgac
      241 tacagtattt aatggatggt ttaggtatag cactttaaat gaagatgaaa aaaattcatg
      301 gctaagcatc ctgaatattt tgtgaatttt cgacataaag aggataatgt aacttggtgg
      361 aacgatttta ataaactaga tgataaagat tatggtaccg tgaaatgggt aaatggtaaa
      421 agtcataaaa tagagagttg gaaatttact gatgatggta agttaaaaga tgaaaaaggt
      481 aacatcgtaa atcctaagtc accagctgtt cagtctgttc tttatgaaga agttcatttt
      541 caaaaagcta aagctaaatt aaaaaaatct ggaggcaaac ttagtcatag tgaaaaagta
      601 tatttagatt ctgaacaggc tatatttatt gctaacggtt taaccacggc atctcaaaca
      661 gcttctgatg atatcaagaa gaatgctgaa ttagttaaag aaaaagcatc agaactattt
      721 gcaaaaacta aagtgatgcc tcctggtata acagatttaa gtccggaaga attagctgat
      781 acatatagtg agggcggagt tagagaggac accatagtaa caccaattga aactttcttt
      841 gatgaaaaag ttactaatgc acaagagatt acaacttcat atattaatct tcaaaaacaa
      901 attgaatctg gtgtccaaaa attgctagaa gaggactcca aattagcagg agaatttaag
      961 gaatggtcac agtactagat caaaatgaaa acaaaattaa tagtctaaaa ataaacctag
     1021 ggaaattgga agataattat catcgcgaaa atatggagat agaagataaa ttatatagcc
     1081 ttgaggataa aaagaacgag ttagagactt atttacaaga agtctatgaa gagacaagtc
     1141 accttttgag acaaaatgat tcagatggtt cgacttttat gagtttaaat cgaataattg
     1201 aaagttatca aatggaatta attgatgaat atgaaaacta taaacagact atcattgtcc
     1261 aagaggaaaa tagtcggaca cagtttctaa aagaccgagt aaaattagaa aatgacattt
     1321 ctagtttaga aggaagaaaa tactactaat tcagatggac tataaagtga aagaggcaat
     1381 aaatgagttt aataggcgct gtattgaaag gaaccaaaga agacaaagaa ttcaaaaagc
     1441 aaattcaaag agctttagta aggattgtct atgcccctga aatagtagaa gaagctgatt
     1501 ctattaagat gacttatagt gacaagattg ataatgtttc tgagacggcc aaatcatcaa
     1561 ttaaaacgat agcaaatcaa atagattcag gacttgtagg acaatttggg gaaaaagtaa
     1621 aggcacaggt agatttaaat aaaggtagtt atgatgattt ataatcgagt gaaaatgttg
     1681 aggaagctta ctatcctgat agttattata gatatgaaaa taatcaagtt ggattagaag
     1741 acataacatt ttacatgaag gataacaaag tttattattt acattttggt aaagcaatag
     1801 ataaaaatga agctaaaaat gataagtatc tcaatcaaat tataaataat taatttcttt
     1861 attttataag ccccgaaaac aaattttttg ataacctttt gataacccct ccgcgaacat
     1921 attgtaatac atagtgcact tttgggatat aaattgt
//
LOCUS       DATRMA010000132         1912 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.134, whole genome shotgun sequence.
ACCESSION   DATRMA010000132 DATRMA010000000
VERSION     DATRMA010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1912
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.134"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            complement(<1..1256)
                     /locus_tag="VBC28_001955"
     CDS             complement(<1..1256)
                     /locus_tag="VBC28_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEO6815901.1"
                     /translation="MNTKKLLQSGFIYTLGNLLVQGLAFITLPIYTRVISTEVYGQYS
                     LYVAWMNIIMLFIGLQTSGSLSSARVKYGEEFKSYSGSAFSVGNIWFLIILLIAFLFR
                     SFLAPLVGFSESIFLLMVCQSYASYVVTFFGQYFIQQQRSLANLILSLANAVSSVALS
                     LFLIFHWSDDFLSRVLGAFVPTIITGIVAFAYIYYHSKSFYNPKYFRFIVTVSIPLIF
                     HLLGHQLLNQLDRIMLVRLYSTKEVAMYSFGYSLGMIIQIVLNSINMAWIPWFFDARK
                     EKLLQLSTYISRYLYLGVFLTLGYLTVFPELAQIMGGDKYSSSVQFISLIIVSYFLVF
                     LYTFPVNIQFFYANTTWIPIGTLLAAGVNWLLNLVLIPHYAAYGAAMATIISYLALLI
                     FHHIVSKVKYHYSDVSVRQYIILSGIV"
     gene            complement(1253..>1912)
                     /locus_tag="VBC28_001956"
     CDS             complement(1253..>1912)
                     /locus_tag="VBC28_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2,3-N-acetylneuraminyltransferase"
                     /protein_id="HEO6815902.1"
                     /translation="NDYTQLGYFFHRNAIPYHLMEDGYNSLKHPTSIAPDGLCDKIKG
                     KITNTPRYHGFSKYCKTIEVSSLKDLPTDERMSKFIEKPKDGLFSDLDNKTKESILKI
                     FDIASIDIEIASPSALILTQPLEEHFKSQEEIYQFYKSIVEEYIDKGYHIYIKVHPRD
                     NIDYSNLNATVIQRNIPIELFDFLENIKFDVGITYFSTALDFLNCVNKKIFLYNIKDI
                     K"
BASE COUNT          732 a          320 c          279 g          581 t
ORIGIN      
        1 actataccac ttaaaatgat gtactgccta actgagacat ctgaataatg gtatttaact
       61 ttcgaaacaa tatgatgaaa aatcaacaag gctaaataag atattattgt agccatagca
      121 gcgccatagg ctgcataatg aggaattaat acaaggttta gtaaccaatt tacaccagca
      181 gctaataatg tcccaatagg aatccatgtc gtattagcat agaagaattg gatatttaca
      241 ggaaatgtat agagaaagac taaaaagtag ctgacaataa ttaaagaaat aaattgaaca
      301 ctactactat acttgtctcc tcccattatt tgagctaact caggaaaaac agtcaaataa
      361 cctaatgtta agaaaactcc taaatagaga taacggctaa tataagttga aagttgcagt
      421 aatttttctt ttctagcatc aaaaaaccat ggaatccaag ccatattaat actattcaaa
      481 acaatctgaa taatcattcc aagcgaataa ccgaaactat acattgcaac ttctttagta
      541 ctatataagc gaactagcat aatcctatct aattgattta ataactgatg tcctaacaaa
      601 tgaaaaatca aaggaataga cacagtgaca atgaaccgaa aatacttagg attgtaaaaa
      661 gatttgctat gataataaat ataagcaaag gcaactattc cagttattat agtaggaaca
      721 aaagctccca aaaccctaga taaaaagtca tcggaccaat gaaaaattaa aaatagagat
      781 agtgcaacag atgaaactgc attggctaag gataatatta aattagccaa actcctctgt
      841 tgttgtataa aatactgacc aaagaaagtc accacatagc tagcgtaact ttgacacacc
      901 attaataaaa aaatagattc agaaaaacca actaatggtg caagaaaact tctaaataga
      961 aaagctatca aaagtataat aagaaaccat atattaccta cagaaaaggc actcccagaa
     1021 tagcttttaa attcttctcc gtatttgacc cgggctgaac ttaaagaacc gctcgtctga
     1081 aggccaataa aaagcataat aatattcatc caagcgacat ataaactata ttggccataa
     1141 acttctgttg agatgacccg agtgtagatg ggaagtgtga taaaggctaa tccttgcacc
     1201 aataaattcc cgagtgtata aataaaacca gattgaagta atttcttagt attcatttaa
     1261 tatccttaat attgtataag aaaatttttt tattaacaca atttaaaaaa tctaatgccg
     1321 tggaaaaata tgtaatccca acatcaaatt ttatattctc taggaaatca aataattcta
     1381 tagggatgtt cctttgtatt actgttgcat tgagattaga gtagtcaatg ttatctctag
     1441 ggtgaacttt aatatatatg tggtaacctt tatcaatata ttcttccact atgcttttgt
     1501 aaaattggta tatttcctct tgacttttaa aatgttcctc taaaggttga gttaatatta
     1561 gagcagatgg agaagctatt tcaatatcta ttgaggctat gtcaaaaatc tttaaaatag
     1621 attccttagt tttattatct aaatcagaaa ataatccatc cttgggcttc tcaataaact
     1681 tagacattct ctcatctgtt ggtaaatctt tcaaactact tacttctatc gtcttacaat
     1741 acttactgaa gccatgatat cggggtgtat tcgttatctt cccttttatc ttgtcacaaa
     1801 gaccatctgg agcgatactc gttggatgtt ttaaactatt atatccatct tccattaaat
     1861 ggtagggaat agcattccta tgaaaaaaat atcctaattg cgtatagtca tt
//
LOCUS       DATRMA010000133         1858 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.135, whole genome shotgun sequence.
ACCESSION   DATRMA010000133 DATRMA010000000
VERSION     DATRMA010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1858
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.135"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            complement(27..398)
                     /locus_tag="VBC28_001957"
     CDS             complement(27..398)
                     /locus_tag="VBC28_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEO6815903.1"
                     /translation="MKKKLLGLMILAISTVFLVACSTNSLNVKYYKVYDGEKMLVLEI
                     NDESGIYHNEGNFVVTNIDTKSKSFVLKGGSAKYTVLYDVKSDGKLNVDLGNFYYNAS
                     GKETYYKEGSKALEKALRDKE"
     gene            complement(551..739)
                     /locus_tag="VBC28_001958"
     CDS             complement(551..739)
                     /locus_tag="VBC28_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system, toxin component, MazF
                     family protein"
                     /protein_id="HEO6815904.1"
                     /translation="MSDIEVYSVLVSRIEYSDGTGSKVRPAVVVKFNDEVIKTLRFTT
                     KYENKSDNIRSQYLEVID"
     gene            complement(736..1029)
                     /locus_tag="VBC28_001959"
     CDS             complement(736..1029)
                     /locus_tag="VBC28_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HEO6815905.1"
                     /translation="MSTVAVRVDDQLKDDATELFQSLGLDMSTAVKMFLIQSVKTQSI
                     PFEIKNKSSVSDEEFQNLVETKLKGIRVKASDPESVNAFFGDEDFSEYEEYFK"
     gene            1086..1235
                     /locus_tag="VBC28_001960"
                     /pseudo
     CDS             1086..1235
                     /locus_tag="VBC28_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000191693.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            complement(1741..>1858)
                     /locus_tag="VBC28_001961"
     CDS             complement(1741..>1858)
                     /locus_tag="VBC28_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001920755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEO6815906.1"
                     /translation="KGNKLPATGENATPFFNVVALTIMSSVGLLSVSKKKED"
BASE COUNT          667 a          344 c          195 g          652 t
ORIGIN      
        1 aataagttag gaaaatagtc ctaactttat tctttatctc ttaatgcttt ttctaaagcc
       61 ttgctacctt ctttataata tgtctcctta ccagaagcat tataataaaa gttccctaaa
      121 tcaacattca atttaccatc acttttaaca tcatataaaa cagtatactt agcagaccca
      181 cctttaagta caaaactttt acttttagta tcaatattag ttactacaaa gtttccttca
      241 ttatgataaa ttcctgattc atcattaatt tctaaaacta acattttttc accatcatat
      301 actttataat actttacatt taacgaatta gtcgaacaag caactaaaaa tactgtagat
      361 atagctaaaa tcatcaatcc taataatttc tttttcataa ctaaacttcc tttgctatga
      421 gaaaatcttt taacctctca acatctctat cgcttaactt acctataatt ttaatattga
      481 accctttatt ctcaacatca taatattgaa tagtatctat ccaagaacgc ctatttaaat
      541 ttgcttttgc ctaatcaata acttctaaat actgacttct tatattgtca gacttatttt
      601 catatttagt agtaaatctt aaagttttta ttacttcatc attaaatttg actactacag
      661 caggacgaac ctttgaacct gtaccatcag aatattcaat acgagaaact aatacagaat
      721 atacctcaat atcactcatt taaaatattc ctcatattct gaaaaatctt catctccaaa
      781 aaacgcattt acactttctg gatcactagc cttaactcgt attcccttta attttgtttc
      841 taccaaattt tgaaattctt catcagaaac agatgattta ttttttattt caaaaggaat
      901 actttgagtt ttaacagatt gaatcaaaaa catttttact gctgtgctca tatccaaccc
      961 taaggattga aaaagttctg tagcatcatc ttttaattga tcatcaactc ttactgctac
     1021 tgttgacata atatcacctc aaatcatttt tgtataacta ttgtatcact aaatgcaata
     1081 caacgcaaac aaaaaaggaa atcgtctttc ccaatgaaga ggctctagaa tactatctag
     1141 tcacactatt taatgattac aacttcaagc aagaacaacg aatccacaaa ggttttggcc
     1201 aatgtacaga cacacttgaa agcctatttg attaacaatc ccagctttgc cagtgtgttt
     1261 acacaacatt attgacacac tccttttatt tttataaatt acccttaaat acatttaatt
     1321 acttgaaaag acaaacagtc attttagaaa attttgcatc taaccttaca tttgattaca
     1381 cctaaaaatt ttaaatcatc aaaagagagc ctaggattaa catcttaggc tctaatgata
     1441 cacttcattt acaaatttca actaaatagg atcatttctt cagttttata gtaagcgtcc
     1501 aataacaagg tatattggat gcttacggaa tagggaagaa aatggaagat acctcgttat
     1561 tggacgctta cacaaaatca aaatcctatc gaaattaata atctcgatag gatttttcaa
     1621 gaattaaact cgaaaaattg aaacacttta attaagtttg ttgaagagag atacataact
     1681 ttattaatgt gttcacacca ataaatggtg aaaagtttag tgacatttta ggtcaaaaga
     1741 ttaatcctct tttttcttag aaacagataa taaaccaact gatgacataa ttgtcaaagc
     1801 tacaacatta aagaatggag ttgcattctc acctgttgct ggtagtttat ttccttta
//
LOCUS       DATRMA010000134         1825 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.136, whole genome shotgun sequence.
ACCESSION   DATRMA010000134 DATRMA010000000
VERSION     DATRMA010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1825
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.136"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            <1..915
                     /locus_tag="VBC28_001962"
     CDS             <1..915
                     /locus_tag="VBC28_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEO6815907.1"
                     /translation="YIAVCILDSSEKDCYDLKHNSLRIINKDALTSELTCLTVDQAFI
                     NIPIELFGKYQIQDIINDIEAMGVIVNVNVEALSFDNIGEKRIQTFEGYSVITYSMKF
                     YKYSHLIAKRFLDIMGAIIGLLICGIVAIFLVPQIRKDGGPAIFSQNRVGRNGRIFRF
                     YKFRSMRVDAEQIKKDLLVHNQMTGLMFKLDDDPRITKIGKFIRKTSIDELPQFYNVL
                     KGDMSLVGTRPPTVDEYEKYNSTQKRRLSFKPGITGLWQISGRNNITDFDEIVKLDVQ
                     YINEWSIWSDIKIILLTLKVVLLGTGAK"
     gene            939..1388
                     /locus_tag="VBC28_001963"
     CDS             939..1388
                     /locus_tag="VBC28_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--LPS N-acetylglucosamine
                     transferase"
                     /protein_id="HEO6815908.1"
                     /translation="MKICLVGSSGGHLAHLNLLKPIWEKEDRFWVTFDKEDARSILRE
                     EIVYHCFFPTNRNVKNLVKNTILAFKVLRKERPDVIISSGAAVAVPFFYIGKLFGCKT
                     VYIEVFDRIDKPTLTGKLVYPVTDKFIVQWEEMKKVYPKAINLGGIF"
     gene            1388..>1825
                     /locus_tag="VBC28_001964"
     CDS             1388..>1825
                     /locus_tag="VBC28_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug MFS transporter"
                     /protein_id="HEO6815909.1"
                     /translation="MIFITVGTHEQQFNRLIKEVDRLKGTGAIDQEVFIQTGYSDFEP
                     QNCQWSKFLSYDDMNSYMKEAEIVITHGGPATFMNAVSKGKKTIVAPRQEQFGEHVNN
                     HQVDFVNKVKTMYNFDIVVDIERLQNVVYEGTMNRPFLETNRSN"
BASE COUNT          633 a          243 c          370 g          579 t
ORIGIN      
        1 tatatcgctg tctgtatctt ggactcctct gaaaaggatt gttatgattt gaaacataac
       61 tcgttaagga taataaacaa agatgctctt acttcagagt taacctgctt aactgttgat
      121 caagctttta ttaacatacc cattgaatta tttggtaaat accaaataca agatattatt
      181 aatgacattg aagcaatggg agtgattgtc aatgttaatg tagaggcact tagctttgat
      241 aatataggag aaaagcgaat ccaaactttt gaaggatata gtgttattac atattctatg
      301 aaattctata aatatagtca ccttatagca aaacgatttt tggatatcat gggtgctatt
      361 ataggtttgc tcatatgtgg cattgtggca atttttctag ttccgcaaat cagaaaagat
      421 ggtggaccgg ctatcttttc tcaaaataga gtaggtcgta atggtaggat ttttagattc
      481 tataaattca gatcaatgcg agtagatgca gaacaaatta agaaagattt attagttcac
      541 aatcaaatga cagggctaat gtttaagtta gacgatgatc ctagaattac taaaatagga
      601 aaatttattc gaaaaacaag catagatgag ttgcctcaat tctataatgt tttaaaaggt
      661 gatatgagtt tagtaggaac acgccctccc acagttgatg aatatgaaaa gtataattca
      721 acgcagaagc gacgccttag ttttaagcca ggaatcactg gtttgtggca aatatctggt
      781 agaaataata ttactgattt tgatgaaatc gtaaagttag atgttcaata tatcaatgaa
      841 tggtctattt ggtcagatat taagattatt ctcctaacat taaaggtagt cttacttggg
      901 acaggagcta agtaaaggta aggtttgaaa ggaatataat gaaaatttgt ctggttggtt
      961 caagtggtgg tcatctagca cacttgaacc ttttgaaacc catttgggaa aaagaagata
     1021 ggttttgggt aacctttgat aaagaagatg ctaggagtat tctaagagaa gagattgtat
     1081 atcattgctt ctttccaaca aaccgtaatg tcaaaaactt ggtaaaaaat actattctag
     1141 cttttaaggt ccttagaaaa gaaagaccag atgttatcat atcatctggt gccgctgtag
     1201 cagtaccatt cttttatatt ggtaagttat ttggttgtaa gaccgtttat atagaggttt
     1261 tcgacaggat agataaacca actttgacag gaaaattagt gtatcctgta acagataaat
     1321 ttattgttca gtgggaagaa atgaaaaaag tttatcctaa ggcaattaat ttaggaggaa
     1381 ttttttaatg atttttatca cagtggggac acatgaacag cagttcaacc gtcttattaa
     1441 agaagttgat agattaaaag ggacaggtgc tattgatcaa gaagtgttca ttcaaacggg
     1501 ttactcagac tttgaacctc agaattgtca gtggtcaaaa tttctctcat atgatgatat
     1561 gaactcttac atgaaagaag ctgagattgt tatcacacat ggcggcccag cgacgtttat
     1621 gaatgcagtt tctaaaggaa aaaaaactat tgtggctcct agacaagaac agtttggaga
     1681 gcatgtgaat aatcatcagg tggatttcgt taataaggta aaaacaatgt ataattttga
     1741 tatcgttgta gatattgaaa ggttacaaaa tgtagtctat gaggggacga tgaatcgtcc
     1801 gtttttagaa actaacagaa gtaat
//
LOCUS       DATRMA010000135         1719 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.137, whole genome shotgun sequence.
ACCESSION   DATRMA010000135 DATRMA010000000
VERSION     DATRMA010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1719
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.137"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            complement(403..525)
                     /locus_tag="VBC28_001965"
     CDS             complement(403..525)
                     /locus_tag="VBC28_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000472358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEO6815910.1"
                     /translation="MFKGFKKLIIIMGGISLMMILTACDLVKKITNGFKNIENT"
     gene            complement(927..1085)
                     /locus_tag="VBC28_001966"
                     /pseudo
     CDS             complement(927..1085)
                     /locus_tag="VBC28_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000926392.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4176 domain-containing protein"
     gene            complement(1325..1702)
                     /locus_tag="VBC28_001967"
     CDS             complement(1325..1702)
                     /locus_tag="VBC28_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000479581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6815911.1"
                     /translation="MFLNNRNFGHVQSIYYTEMAKMYIMIMLIIFIVFSGIYYFYYLP
                     KNQGKKWKFNQWKLQKGKKEQLKINYGIIFGLVMLSPVFLVGSIENIFGLIGGVLFTA
                     TFPALIVDSLYAAYYIHKHPEYE"
BASE COUNT          595 a          299 c          202 g          623 t
ORIGIN      
        1 aaaacaacta cttaaggatg atagatactt tcactaaaaa attttgatta atcaaatata
       61 ttattattat agcatatcaa atattatttt ttacatattt attaattaat atgtaacacc
      121 cattatctga agtgtgtagg tgaggtctaa gtaaagaatt taagcgtttt gataataaaa
      181 aagtgagaac aatagattct cactcacaat tagcctggta atttctcaca cgatttggta
      241 ctgcttttta aaattgggcg atggtgttat ttttaatatg agtagctgca ctcgaatccg
      301 attgctcaaa agcacttgcc gctccattga gatagctatt cagatcagaa ataactggtt
      361 gaatgttttg gctgacttct ttgataactt ttgatccagc ttctaagtat tttcaatgtt
      421 cttgaaccca ttggtgattt tttttactaa gtcacatgct gttaaaatca tcattaagct
      481 tatccctccc ataataataa tcaatttttt aaatccttta aacatcactt ttcctcttat
      541 cttattgatt ttttcaattt tattgctgtc ttgacgttgc tatcgcaaac aaccaactca
      601 atgcaatata gttatggtat acatatcaat gtaagaatca agatttttaa attcatatta
      661 tttgtatgat attcaggatt agctattttt gatttcacaa aaaaaacttc ttctactttt
      721 tctagactga ttctttcttc ccactccttc gtatcaatag ttaaacaaag tactttaaat
      781 cctacagagg gtcattccat caattaatgg tatcatcgcg cttgaagcat ggcataggtt
      841 ttggtattag aaactgaata tgaattctat tatctgttaa aaaaatcaat atcaccagag
      901 tatttataaa acataacgat aaaaaatcaa aaaatatttt tccaattttc aatgacacga
      961 gctactcttt cttgctcttc gtcatggtac ctccaaaaat tacttcatca atatcttcct
     1021 cattgaaata atatacttgt tctaaattga gctctctgga aaaatcctgc cagataatca
     1081 aaatatcatc ttgatcaata atatttgctc atgaaatggc tccacaaaat ttcttacatc
     1141 tttaacattt tcatagagtg cttttactcc acaatggcca aagtacctat aacaactgct
     1201 actactggta caaattggag ctgttatgat tactacaaaa acactctcag caagcgtaga
     1261 gactattcta cctacttcat tttttctaga tatttattgc tcctacctta aattaattaa
     1321 tttatcactc atattcggga tgcttatgaa tataatatgc tgcataaagg gaatcgacaa
     1381 tcagagctgg aaaagttgcc gtaaataata cacctccaat caaaccaaaa atattttcaa
     1441 tactacctac aagaaagact ggagaaagca tcactaaacc aaaaataatt ccataattaa
     1501 tttttaattg ttcctttttt cctttttgta atttccactg attaaatttc cattttttcc
     1561 cctgattttt tggtaaataa tagaaataat atattccaga aaatacaata aaaataatta
     1621 acattatcat aatatacatt tttgccatct cagtataata aattgattga acatgtccaa
     1681 aatttctatt atttaaaaac attgccaagc ctataaaat
//
LOCUS       DATRMA010000136         1695 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.138, whole genome shotgun sequence.
ACCESSION   DATRMA010000136 DATRMA010000000
VERSION     DATRMA010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1695
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.138"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            complement(1..1358)
                     /locus_tag="VBC28_001968"
                     /pseudo
     CDS             complement(1..1358)
                     /locus_tag="VBC28_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200920.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1348..>1695)
                     /locus_tag="VBC28_001969"
     CDS             complement(1348..>1695)
                     /locus_tag="VBC28_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6815912.1"
                     /translation="GQTERHRIIKGYSDGMKSKLSLICLMISKPKVILLDEPLTAVDV
                     VSSIAIKRLLLELSEDHIIILSTHIMALAEDLCDIVAVLDKGKLQTLDIDRKHEQFEE
                     RLLQVLKGDEYDK"
BASE COUNT          626 a          288 c          256 g          525 t
ORIGIN      
        1 tctgatcaat cttaggggtg aaaaaaatta aaataatagg ttccaaaaca tatgtttaaa
       61 gctacaaaaa tggggaaatt aagttttata ctataaagga aacgtatttt taaggttgct
      121 aagatatttt ctctttcacg atagaaatta tatcttaata atggacgatc caaatgatag
      181 aaacaaaatt ttgaatagga ttcaccgata tatattagat atcctggaac tgcactcaaa
      241 aataggaatt ttgaaaaact atgctcattc atcggaatat ttatcttttc tttaaaaaat
      301 agcaggaaaa ggagttctat tccaaacaga acacttaaaa cagctaaacg taccaaaatt
      361 ttcctttgca agtatatact catccgtttt aaaaagatag cgtttaaata tgaaatacct
      421 gataaatttt caaatagcag gggagaaagt tcactgacat ttatatccct atctttcatt
      481 tcaatcccaa gcagctctat attatcaatc tttgcaattt tctccttcat cgtctcatgc
      541 gttagggtaa ggtaggtcaa gtggttaagt ttagaataag tttttaatcg atagtaacta
      601 actaaccata tgataagtcc tgcaataccg gtgtaccatg aaaaaaatgg atatggagag
      661 aactgtttgc caagaagaag gaaaaagaga gaaaggaaaa ctcctatacc aatccctaag
      721 taaaataaaa tatttagtaa agactctgga ttctcttggt ctttacctct accatataaa
      781 ggtaaacaga gcaccttgat tccaaggcga ctacctgcta aaaataaagc aaaagtcaaa
      841 ccatgccaaa aagcaaagtg atttaaatgg aaaaatatgc caaagctcaa acttctaaaa
      901 aatagttcac tactggcttc taaaagttca gcggattgga aatacctcga tggctcaatt
      961 ctgaaaaggc gaatcatatg agtttgttgc atatcattag gttctataag ttttatatag
     1021 aaatttatga caattaaaag agaggtaatc accatcaatg ttaaagttag ggtaaaacct
     1081 tcttgcggta aaatatttaa ctgcttcatg ccacttctaa caaaaaatga taaaacataa
     1141 caatatagta cgtaacctat ggaacgacca aaacctctta agaaaaagaa aatgatacga
     1201 ataaatagta ctacaccttt tatatcatcg gttttaaacc atcggttagg gatttttctt
     1261 cccaaccagg ggattttctg aaggtaatat aaaaaactat tataagctct agcactatca
     1321 atctgtaata tagaccatat taatgcttta cttgtcatat tcatctccct tcaacacttg
     1381 aagaagacgc tcttcgaatt gttcatgttt acgatcaata tctaatgttt ggagttttcc
     1441 tttgtctaat acagccacaa tatcacatag atcttctgct aaggccatta tatgagttga
     1501 taatataata atatgatcct cacttaattc caacaaaagg cgttttattg caatacttga
     1561 tacaacatca actgcagtca gtggctcatc tagtaaaatt acttttggct tagaaatcat
     1621 caggcaaatt aatgagagct tactcttcat tccatcagaa tatcctttga taattctatg
     1681 acgctctgtt tgtcc
//
LOCUS       DATRMA010000137         1593 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.139, whole genome shotgun sequence.
ACCESSION   DATRMA010000137 DATRMA010000000
VERSION     DATRMA010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1593
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.139"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            143..568
                     /gene="rplK"
                     /locus_tag="VBC28_001970"
     CDS             143..568
                     /gene="rplK"
                     /locus_tag="VBC28_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004226069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEO6815913.1"
                     /translation="MAKKVEKLVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGMIIPVVISVYEDKSFDFITKTPPAAVLLKKAAGVEKGSGEPNKTKVATITR
                     AQVQEIAETKMPDLNAANLESAMRMIEGTARSMGFTVTD"
     gene            672..1361
                     /gene="rplA"
                     /locus_tag="VBC28_001971"
     CDS             672..1361
                     /gene="rplA"
                     /locus_tag="VBC28_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEO6815914.1"
                     /translation="MAKKSKNLRAALEKIDSTKAYSVEEAVALAKETNFAKFDATVEV
                     SYNLNIDVKKADQQIRGAMVLPAGTGKTSRVLVFARGAKAEEAKAAGADFVGEDDLVA
                     KIQGGWLDFDVVIATPDMMALVGRLGRVLGPRNLMPNPKTGTVTMDVAKAVEESKGGK
                     ITYRADKAGNVQALIGKVSFDDAKLVDNFKAFNDVIVKAKPATAKGTYITNLSITTTQ
                     GVGIKVDPNSL"
BASE COUNT          544 a          267 c          301 g          481 t
ORIGIN      
        1 ttaaaatata atgaaattaa tggcttaaaa ctattgcttt tatttatgat tatagtataa
       61 tggtaaggta tgttataaac atgcctgtgg gaggtaaaaa tttctaatta ccgccaaaac
      121 cacaacagga ggatttttaa aaatggctaa aaaagttgaa aaacttgtaa aacttcaaat
      181 tcctgctggt aaagcaactc cagctccacc agttggacca gcacttggtc aagcaggaat
      241 caacatcatg ggattcacta aagagtttaa cgctcgtaca gctgatcaag ctggtatgat
      301 tattccagtt gttatctcag tatatgaaga caaatcattt gatttcatca ctaaaacacc
      361 accagctgct gttcttttga aaaaagctgc aggtgttgaa aaaggatcag gtgaacctaa
      421 caaaactaaa gttgcaacaa ttactcgtgc gcaagtacaa gaaattgctg aaactaaaat
      481 gccagattta aacgctgcaa accttgagtc tgcaatgcgt atgatcgaag gtactgctcg
      541 ttctatggga ttcactgtta ctgactaatc agaaacaccc acataacccg caagacttca
      601 tcgtagtttg atgagtgacg tgggagattt taaatcaaat cgattgacca cattacaagg
      661 agaattttaa aatggctaaa aaaagtaaaa acttacgtgc tgctcttgag aaaattgaca
      721 gcactaaagc atacagcgta gaagaagctg tagctcttgc aaaagaaact aactttgcta
      781 aatttgatgc aactgttgaa gtttcttata accttaacat tgacgttaaa aaagctgacc
      841 aacaaatccg tggtgctatg gttttaccag caggtactgg taaaacttca cgtgttcttg
      901 tttttgctcg tggtgctaaa gctgaagaag ctaaagcagc aggtgcagac tttgtcggtg
      961 aagatgactt agttgctaaa atccaaggtg gatggcttga ctttgatgtt gttatcgcaa
     1021 caccagatat gatggcactt gttggacgtc ttggacgtgt ccttggacct cgtaacttga
     1081 tgccaaaccc taaaactggt acagtaacta tggacgttgc taaagcagtt gaagagtcta
     1141 aaggtggtaa aattacttac cgtgctgata aagcaggtaa cgtccaagcc cttattggta
     1201 aagtttcatt tgatgatgct aaattagttg ataacttcaa agcatttaat gatgttattg
     1261 ttaaagctaa accagctact gcaaaaggaa cttacatcac aaacctttca attacaacaa
     1321 cacaaggtgt tggtatcaaa gttgatccta actcacttta atcaataaaa aactaggaat
     1381 tccttcctag ttttatttat tctatatact taactagtta agtatatatt ttaataatca
     1441 aaggatatta tatgtgtttc ttttgaaaaa tagcttttta tctgtattaa ccttcttaaa
     1501 gactaatgaa tgtgcgatgt gttttaagta acagatgaga ataaggaaaa atgaaattgg
     1561 cgatatggta tgtgactatt atgattattc ttt
//
LOCUS       DATRMA010000138         1532 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.141, whole genome shotgun sequence.
ACCESSION   DATRMA010000138 DATRMA010000000
VERSION     DATRMA010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1532
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.141"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            <1..640
                     /locus_tag="VBC28_001972"
     CDS             <1..640
                     /locus_tag="VBC28_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="capsular biosynthesis protein CpsC"
                     /protein_id="HEO6815915.1"
                     /translation="KLWKKKFLITFVAIAFATAGLFYSLFIVTPQYTSSTRIYVINPN
                     TPNNSITAQDLQAGSFLANDYKEIITSTDVLEKVISSEKLNYPSSQLLQKITVSILKD
                     TRVISISVEDANPKMSQKLANSVREAAVSKIKAVTQVEDITTLEKGNLPKAPSSPNIK
                     KNVLIGFIVGAGLSTIVLVIMGILDDRVNTEEDIEKALGLTSLGIVPDLNKL"
     gene            651..1349
                     /locus_tag="VBC28_001973"
     CDS             651..1349
                     /locus_tag="VBC28_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000197400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HEO6815916.1"
                     /translation="MTRLEIVDSKLRQAKKTEEYFNAIRTNIQFSGKENKILAITSVR
                     EGEGKSTTSTSLALSLAQAGFKTLLIDADTRNSVMSGTFKATGTIKGLTNYLSGNADL
                     GDIICETNVPRLMVVPSGKVPPNPTALLQNAYFNKMIEAIKNIFDYIIIDTPPIGLVV
                     DAAIIANACDGFILVTQAGRIKRNYVEKAKEQMEQSGSKFLGIILNKVSESVATYGDY
                     GDYGNYGKRDRKRK"
BASE COUNT          539 a          221 c          271 g          501 t
ORIGIN      
        1 aaaattgtgg aaaaagaaat ttttaatcac atttgttgcc attgcatttg caacagctgg
       61 acttttctat agtcttttta ttgttacgcc acaatatact tcttcaacaa ggatatatgt
      121 cattaaccct aataccccta ataatagtat tactgcacaa gatctacaag cggggagttt
      181 tcttgcaaat gactataagg agattattac gtctactgac gttctagaaa aagttatttc
      241 ttctgaaaaa ttgaattatc cttcgtctca gttgttgcaa aaaataacag tttctatttt
      301 aaaagataca cgtgttattt caatatcggt cgaagatgct aatccaaaaa tgtctcaaaa
      361 attagcaaat tcagttagag aagcagcagt ttcaaaaatc aaggcagtta ctcaagtaga
      421 ggatatcact actcttgaga agggaaattt acctaaagca ccatcttctc ctaatattaa
      481 aaagaatgta ctaatcgggt ttattgttgg tgcaggatta tcaactattg ttttagttat
      541 tatgggtatt ttggatgatc gtgttaatac tgaagaagat attgaaaagg ccctaggact
      601 tacttcttta ggtatagtac cagatttgaa taaactttaa ggagaatata atgactcgtt
      661 tagaaatagt tgatagcaag ttaagacaag caaaaaaaac agaagaatac ttcaatgcga
      721 tccgtacaaa catacagttt agtggaaagg aaaataaaat tcttgcaatt acctctgtta
      781 gggaagggga aggaaaatcc actacttcaa caagtttagc tttatcttta gctcaagcag
      841 gatttaaaac attattaatt gatgcggata ctaggaactc tgttatgtct ggaaccttta
      901 aagcaactgg aactattaaa ggcttgacga attatttatc aggtaatgca gatcttggag
      961 atattatctg tgaaaccaat gttcctagac tgatggtcgt tccttcaggg aaagtaccac
     1021 caaatccaac agcattactt cagaacgctt attttaataa gatgattgaa gctattaaaa
     1081 atatatttga ttatattatc atcgatactc cacctattgg tttagttgtt gatgccgcaa
     1141 taatcgctaa tgcttgcgat ggttttattt tagtaaccca agcaggtaga ataaaacgta
     1201 attatgttga aaaagcaaaa gaacagatgg aacaaagtgg ttcaaagttc ttaggtatta
     1261 ttcttaataa agttagtgaa tctgttgcta cttacggcga ttacggcgat tatggaaatt
     1321 acggaaaaag ggatagaaaa aggaagtaag gggctcttgt attgaaagaa aaagaaaata
     1381 tacaaaagat tattatagcg atgattcaaa cagttgtggt ttatttttct gcaagtttga
     1441 cattaacatt aattactccc aattttaaaa gcaataaaga tttattgttt gttctattga
     1501 tacattatat tgtcttttat ctttctgatt tt
//
LOCUS       DATRMA010000139         1527 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.142, whole genome shotgun sequence.
ACCESSION   DATRMA010000139 DATRMA010000000
VERSION     DATRMA010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.142"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            411..596
                     /locus_tag="VBC28_001974"
                     /pseudo
     CDS             411..596
                     /locus_tag="VBC28_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000761049.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            665..835
                     /locus_tag="VBC28_001975"
     CDS             665..835
                     /locus_tag="VBC28_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6815917.1"
                     /translation="MEASSGTPQGGEQLLLILGYANGSELTNFTLNFTLGDNQMPNID
                     SVGSDNLSTIED"
     gene            905..1075
                     /locus_tag="VBC28_001976"
     CDS             905..1075
                     /locus_tag="VBC28_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6815918.1"
                     /translation="MAESANTGRSNSFVLEELTEFQREKLKNGESVKFNFSKDVKKEN
                     KVIEGGHPQVIG"
BASE COUNT          569 a          172 c          277 g          509 t
ORIGIN      
        1 ttataaagac aaggtttaga agccttgtct tttttgtatt gtgctggtaa ttttgagaaa
       61 aagttactga aaattactta tatttgtttt tcattattgc aatcgttata gaaggtttaa
      121 aacaagcaga aataataagg aaatttttca agagttagtg gagactcaac atttaaatga
      181 aattcaaaga ggactcctaa atgtcatagg ttctgaagta ataaaagtgt ttgtgaatga
      241 tagataggta gtataaaata ataagatttt tcagtgtata tgttacgact aaaaaattaa
      301 actcgtgaaa atttgttata ctaatattag ttttaacata ctggtaaata ataattattt
      361 ttattagata cttgacatgg gaagtttatt ttctaagaaa agagagcatg ttgaaaaaat
      421 atgtaaaatg gttaattccc atatctatta ttgggatgtt actagcaggc tgtcaaatga
      481 atagtgaatc taaaagtcag tcaaacgaag taaaaaatag caaacagtca gcagtaaaga
      541 aaggtaaaaa agtaataaaa aaagaacaag tagcttatct caaaaagcat gaaaaagaat
      601 taactgaata tgtcaaaatc cagatattag tgaaattaag tatgattgga atagtgtgcg
      661 catagtggaa gctagtagtg gaacccctca aggcggagag caactattat taattttggg
      721 ttatgcaaat ggcagtgaat taaccaattt tactctaaat ttcacattgg gtgataatca
      781 aatgcctaat attgactcag ttggctcaga taatttatca actattgaag attaataata
      841 acaataagaa aggtatataa tgggatgatg gaatcttaag gaatttgaag atatattttg
      901 aagtatggca gaaagtgcta atacaggtcg atcgaattct tttgttttag aagaattaac
      961 tgaatttcaa agagaaaagt taaaaaatgg ggagtcagtt aagtttaatt tttctaaaga
     1021 tgttaagaaa gaaaataagg ttatcgaagg agggcacccc caagtcatag ggtaatctac
     1081 ctgcaaactg gataagtcct taaatacaat tgatgaaaaa gtagaggttc gcgttcctga
     1141 tgtcaatggc ggttatcact ctgagcattg tgttactaat tcctatcaga aaggttactg
     1201 aaagatgatg aagcagtaac tctataatca ctaaaagctc atcattgctt agtgtttaag
     1261 ttaacttcta aagctcaaac cttgttaaat caaggtctgt ttgcttttaa aagtgacatg
     1321 ttatttttct aaaaatgaga ttattgggat tcctcattct aagttacttt gtttatatgt
     1381 acctttactt aaggtttatt ttcattattt ttaatttaat attttggagt atgtatgctc
     1441 cattatagta ttttcgaaga catctttaaa aggactgtag aagttaaaat attttaacct
     1501 agtatctaac gtcatatatt ggtacat
//
LOCUS       DATRMA010000140         1515 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.143, whole genome shotgun sequence.
ACCESSION   DATRMA010000140 DATRMA010000000
VERSION     DATRMA010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1515
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.143"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            <1..740
                     /locus_tag="VBC28_001977"
     CDS             <1..740
                     /locus_tag="VBC28_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEO6815919.1"
                     /translation="GSISTVSRSDVNIIMTVNTNTHKVLLTTTPRDAYVKIPDGGGNQ
                     YDKLTHAGLYGVETSMKTLENLYDINLDYYARINFSSFLKLIDLLGGVTVYNDQAFTS
                     KHGNFDFPVGQVTLNSEQALGFVRERYSLQGGDNDRGRNQEKVIAAIINKLASSQSVT
                     KLNSITSQLQTSVQTNMTIDNINDLINNQLSTGQRFTVESQALTGHGSTGELPSYAMP
                     GAQLYMMSIDQSSLSNAKSKIKNTMEE"
     gene            746..1477
                     /locus_tag="VBC28_001978"
     CDS             746..1477
                     /locus_tag="VBC28_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase CpsB"
                     /protein_id="HEO6815920.1"
                     /translation="MIDIHSHIVFDVDDGPKTLEESLSLIEESYRQGVRIIVSTSHRR
                     KGMFETPEDIIFKNFSIVKHEAEKRFEHLQILYGGELYYTSDMLEKLKLKQIPTLNNT
                     KFALIEFSMQTSWKDIHTALSNVLMLGITPVVAHIERYNALENQKERVKEIINMGCYT
                     QINSSHILKQKLFNDKHKRFKKRARYFLEENLVHFVASDMHNLDVRPPFLAEAYKIIC
                     RDFGKERANQLFIENAQSILKNHYI"
BASE COUNT          553 a          228 c          266 g          468 t
ORIGIN      
        1 ttggctctat atcaacagta tcaagatctg atgtaaatat tattatgacg gttaatacca
       61 atacccacaa agtattgtta acgacaacac cacgagatgc ctatgtaaaa attccagatg
      121 gtgggggcaa tcaatatgat aaattaaccc atgcaggttt gtatggcgtt gagacatcaa
      181 tgaaaacact tgaaaacctt tacgacatca accttgatta ttatgctaga attaattttt
      241 catcattttt aaaattaata gacctcttgg gaggagtgac agtttataac gatcaagctt
      301 ttacaagtaa acatggtaat tttgacttcc ctgttggtca agtaacattg aattctgagc
      361 aggctttggg ctttgttaga gaacgttatt ctctacaagg aggcgataac gatagaggta
      421 gaaatcaaga aaaagtgatt gcagctatta taaataagtt agcttctagt cagtcagtaa
      481 caaaattaaa tagcattacc tcacagctcc aaacgtccgt tcaaactaat atgactattg
      541 ataatattaa tgatttgatt aacaatcaat tgtcaactgg acaacgcttc actgtcgagt
      601 cacaagcatt aactggtcat ggttcaacgg gtgaactccc ttcatatgca atgccaggag
      661 ctcaacttta tatgatgtca attgatcaat ctagcttatc taatgcaaaa tcaaaaatta
      721 agaacacaat ggaggaataa caagaatgat tgatattcat tctcatatcg tttttgatgt
      781 agatgatggg cctaaaacgc ttgaagagag tttatcttta atagaagaaa gttatcgaca
      841 aggtgttaga ataattgttt cgacatcaca tagaagaaaa ggtatgtttg aaacacctga
      901 agatattatc ttcaaaaact tttctatagt taaacacgaa gcggaaaaaa gatttgaaca
      961 tcttcaaatc ttatatggtg gagaattgta ctatacaagt gatatgttag aaaaactgaa
     1021 gttaaagcaa attccaactt taaacaatac gaaatttgct ttaattgaat tttctatgca
     1081 aacttcttgg aaagatattc atacagcttt gtcaaatgtt ttaatgcttg gtattacacc
     1141 agtcgttgcg catatagaga ggtataacgc tttagagaat caaaaagaac gggtgaagga
     1201 aattattaat atggggtgtt acacacaaat aaatagttcc catattttga aacaaaaact
     1261 ttttaatgat aagcataaac gctttaagaa aagagcccgt tattttttag aggaaaattt
     1321 agtgcatttt gtagcgagtg atatgcataa ccttgatgtt agaccgccat ttttagcaga
     1381 agcttataag attatctgta gagatttcgg taaagaacgt gctaaccaac tttttattga
     1441 aaatgctcaa tctatattaa aaaaccatta catttaggag atttcatgaa taaaatagct
     1501 aatacagaag ttgaa
//
LOCUS       DATRMA010000141         1484 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.144, whole genome shotgun sequence.
ACCESSION   DATRMA010000141 DATRMA010000000
VERSION     DATRMA010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1484
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.144"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            26..682
                     /locus_tag="VBC28_001979"
     CDS             26..682
                     /locus_tag="VBC28_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6815921.1"
                     /translation="MDILEIKNVNYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKVQTGKILFKNEDIEKKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSVDESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKELAQDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            682..>1484
                     /locus_tag="VBC28_001980"
     CDS             682..>1484
                     /locus_tag="VBC28_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6815922.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMATLSIISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTNSIDSYVKRINSVADLV
                     DHDIIETQDTLANQSPERAKNFKRTVMLTGVNDSAKETKFVSEAYKLVEGKHLENKDK
                     NKILMHKDLAKKNNLKVGDKIKIKSNLFDADNEKVANETVEVEIKGLFDGHNSGGVSA
                     AQELYENTLITDVHSAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLG"
BASE COUNT          634 a          168 c          272 g          410 t
ORIGIN      
        1 atcaaaaata agttaggagc aaataatgga tatattagaa ataaagaatg taaattacag
       61 ttacgcaaat tctaaagaaa aagttttgtc aggagtaaat caaaaatttg aacttggaaa
      121 gttttatgcg atagtaggga agtcaggaac aggaaaatcc acacttcttt ccttacttgc
      181 aggacttgat aaagttcaaa caggaaaaat cttgtttaag aatgaagata tagaaaagaa
      241 aggatatagt aatcacagaa aaaataatat atctttggta tttcaaaatt ataatttaat
      301 agattattta tcgccgattg aaaatattag actagtaaat aaatcagtag atgagagtat
      361 cttgttcgaa ttaggtttag ataaaaaaca aataaaaaga aatgttatga aattatctgg
      421 tggtcagcaa caaagggtag ctattgctag ggcactggta tcagatgccc caataatact
      481 agctgatgag cctaccggta acctagacag tgttactgct ggagaaataa ttaatatatt
      541 aaaggaatta gctcaagata gaaataaatg tgtaatagtt gtaacacata gtaaggaagt
      601 agcagattct gcggatatca ttttagaact aagtggtaaa aagttgaaaa aagtaaataa
      661 aatgaatttg gaggttgaat aatgataaaa aatgcatttg cttatgtaac tagaaaaagc
      721 ttaaaatcat taattattat attagttatt ctatcaatgg caactttaag catcattagt
      781 ttatctatta aagatgccac agatagagct tctaaggaaa catttgctaa tataacaaac
      841 agtttttcta tggaaataaa tagacaagta aatccaggaa cacctagggg tggaggaaat
      901 gtaaaaggtg aagatattaa aaagatatct caaacaaaca gtatagactc ctatgtaaaa
      961 agaataaaca gcgtcgcaga cctagttgat catgatataa ttgaaactca agatacactt
     1021 gcaaatcaat cacctgaaag agcaaagaat tttaagagaa cagttatgtt aacaggggtt
     1081 aatgactcag caaaagaaac aaaatttgta tcagaagcat ataagttagt agaaggaaag
     1141 catttagaaa ataaagataa aaataaaatt ttaatgcata aagatttggc aaaaaaaaac
     1201 aatctcaaag ttggagataa aataaaaata aaatctaatt tgtttgatgc tgacaatgaa
     1261 aaagttgcaa atgaaactgt agaagtagaa attaaaggtt tatttgatgg tcataatagc
     1321 ggtggggtaa gtgcagcaca agaactatac gaaaatacac taatcactga cgttcattca
     1381 gctgctaaag tttatggtaa tacagaagat acagccgtat accaagatgc aacattcttt
     1441 gtaaaaggtg ataagaatct tgatagtgta ataaaagatc ttgg
//
LOCUS       DATRMA010000142         1459 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.145, whole genome shotgun sequence.
ACCESSION   DATRMA010000142 DATRMA010000000
VERSION     DATRMA010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1459
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.145"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            164..1447
                     /locus_tag="VBC28_001981"
     CDS             164..1447
                     /locus_tag="VBC28_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HEO6815923.1"
                     /translation="MSLYYTEKAHAIAGPSDRQYVENPNPHIIVNVTGTDQNGNSILP
                     HYIEVNVKMGQTLSKEEILDYIARNLNSSVGGESKNVQYSNIEFKESAYLKRQLDDGK
                     TEEIAIDNDGVTVPKDGPNKFWIDVPVTCTVTPIVTETHEVRWGTPVAISHRIYFVEE
                     SSGKVLDEYTNLHTADSELNGYRVGDYITDYALSKSAYEAFLNSRLDKEGYKLQHRIS
                     TNVRQNLQIDKLIFNYDFNEENIYYQIGNIRPLLSRSSAEVESDIITERYYVSKNAKS
                     LARTESTISIKMVDAKTEQPLFNHTLTGYQLATVSHVYNRLFEENLIPTTKSGERYFI
                     QNMKKTAEQEYTVYLSETPYSKENAPVISYDARPVDWDYHSGASGSLENQPNIYTEED
                     STEFLGNKPQAACYPNKQFACENTDSKYNYSYLEK"
BASE COUNT          524 a          264 c          239 g          432 t
ORIGIN      
        1 ttatatcagt ataacttagt gtcgtagaga ttaattccta tcattcactg ctttatttta
       61 ggataactaa acaatttatt gtttaaaatg aaaggattta tgaatgtcta ggaaaacatt
      121 taaacatata ctatcgattg gagtttgcac gctcgtacta tcgatgagtc tttattacac
      181 tgaaaaagcc cacgctattg ctggtcctag tgaccgccaa tacgtagaaa acccaaatcc
      241 tcacattatt gtaaatgtta caggtactga tcaaaacgga aatagcattt taccgcatta
      301 catcgaagtc aatgtaaaga tgggacaaac tttaagtaaa gaagaaattc tagattatat
      361 tgctcgaaat ttaaactcta gtgttggagg agaaagtaaa aacgttcaat acagcaacat
      421 cgagtttaag gaaagtgctt atctgaagcg tcaattagat gatggcaaga cagaagaaat
      481 agcaattgat aacgacggtg ttactgtacc taaagacggt ccaaacaaat tttggattga
      541 cgttccagta acttgtactg ttactcctat cgtaacagaa acacatgaag ttcgatgggg
      601 gactccagtc gctatatcac accgtattta ctttgttgaa gaatcttctg gaaaagtttt
      661 agatgaatac acaaatctac acactgctga ttcggaactt aacggttatc gtgttggaga
      721 ttatatcaca gactatgcac tttctaagtc tgcttacgaa gcttttttaa actctcgttt
      781 agataaagaa ggttacaaac ttcaacatcg tattagcacg aatgtacgac aaaaccttca
      841 aattgataaa ttgattttca attatgactt taatgaggaa aatatttact accaaatcgg
      901 gaatatccgt ccactattaa gtcgctcatc agctgaagta gaatctgaca tcattacaga
      961 acgctactat gtttctaaaa atgctaaaag tttagcacgt acagaatcaa ccatttcgat
     1021 taaaatggtt gatgccaaaa ctgaacaacc gctatttaac cacacattaa ctggttatca
     1081 attggcaact gtctcccatg tctataacag actctttgaa gaaaatctta tcccaactac
     1141 aaaatcagga gaaagatatt ttattcaaaa tatgaaaaaa acagctgaac aagaatatac
     1201 tgtttacctt tcagaaacac cttattctaa agagaacgct cccgtaattt cttatgatgc
     1261 aagacctgtt gattgggatt atcactcagg cgcttcagga tcacttgaaa atcagcctaa
     1321 catctatact gaagaagatt caactgaatt tttgggtaat aaaccacaag cagcttgtta
     1381 tccaaacaaa caatttgctt gcgaaaatac tgactctaaa tacaactata gctatttaga
     1441 aaaatagtta taaccgata
//
LOCUS       DATRMA010000143         1454 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.146, whole genome shotgun sequence.
ACCESSION   DATRMA010000143 DATRMA010000000
VERSION     DATRMA010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1454
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.146"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            538..828
                     /locus_tag="VBC28_001982"
     CDS             538..828
                     /locus_tag="VBC28_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WXG100 family type VII secretion target"
                     /protein_id="HEO6815924.1"
                     /translation="MSQIKLTPEELRISAQKYTTGSQSITDVLTVLTQEQAVIDENWD
                     GTAFDSFEAQFNELSPKITQFAQLLEDINQQLLKVADVVEQTDSDIASQINK"
     gene            930..1337
                     /locus_tag="VBC28_001983"
     CDS             930..1337
                     /locus_tag="VBC28_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6815925.1"
                     /translation="MKKSTQIILLIVALFILVFSGGFYMKEQQRKEELKRNREYEVSL
                     VKALKNSYENIEEIKITHPVSTEIPGDWHCTVKISFNDKKSIVYNITHNLESKKNYSG
                     KFNEKNMNFFDSRIGKTKKTIKIIFSDGQEKIQ"
BASE COUNT          567 a          197 c          227 g          463 t
ORIGIN      
        1 ccggtaccgg tatagttcaa ggccttcaaa cattgatttg aagacctttt tccttgtttt
       61 gagcaatttt atattttttc atttaattga gataaaaaag gctccttgtc taataagata
      121 acctctagct cagtattcta tttttttgga agtgttgaga aatttacctt atttaaaaga
      181 aattatttga aattatataa cataaagtga aaagtacata gtgacaaaag tgcggtaata
      241 aagactttta gtatttaaag agatgtaggt gccaccaacc aggtcttaag aaagctcgta
      301 aagcttcaca attctcaaaa tgttaatcag tacgattata tcaacgtttc aaagcacttt
      361 gcaagtcttg caaggtgttt tttaaaacct tttgataacc ctttctgttt tgaaagtaag
      421 gtaaatgtaa tataaatcgc tgtttgtatt tttaatagat taacctatta tgagttttat
      481 ctgtgtaagg aacatctaaa tatgctatac tgcagttaaa aaaataggga gcaaatgatg
      541 tcacaaatta aactaacacc tgaagaactc cgtatttcag cacaaaaata tacaacagga
      601 tcacaatcaa ttacagatgt gttaacagtt ttgactcaag aacaagctgt tattgatgaa
      661 aattgggatg gtacagcatt tgatagcttt gaagcccaat tcaatgaatt atctccaaaa
      721 atcacacaat ttgcacaatt attagaagat ataaatcaac aattattgaa agttgcggat
      781 gttgtcgaac aaacagactc agatattgcc tcacaaatta ataaataatt ggcttattca
      841 aattcataag tttaacttag gaaggcataa gctctcctaa gttgatatat tatagctaga
      901 tgatgtaatt gtttcataaa ggagtaaaaa tgaaaaagag tacccaaata atactactaa
      961 tagttgcatt attcatactt gtttttagcg gaggatttta tatgaaagaa caacaaagaa
     1021 aagaagaact aaaacggaat cgagaatatg aagttagtct agtcaaagca ttgaaaaatt
     1081 cctatgagaa tatagaagaa ataaaaatca cacatcctgt ttcaactgaa attcctggag
     1141 attggcattg tactgtaaag atttcattta atgataaaaa atctattgtt tataatatta
     1201 cacataattt ggaatcgaaa aaaaattata gcggaaaatt taatgaaaaa aatatgaatt
     1261 tttttgattc aagaattggt aaaacaaaaa aaactataaa aattattttt tcagatggtc
     1321 aggagaagat acaataatgg tatttacata taccgagaaa gaacttcgtg aattcaatat
     1381 tggagataat gtatattcag ttaaccccga ttatgcagaa aaaaatcatt caactgtcat
     1441 aacggattta cccc
//
LOCUS       DATRMA010000144         1429 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.147, whole genome shotgun sequence.
ACCESSION   DATRMA010000144 DATRMA010000000
VERSION     DATRMA010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1429
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.147"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            <1..701
                     /gene="recX"
                     /locus_tag="VBC28_001984"
     CDS             <1..701
                     /gene="recX"
                     /locus_tag="VBC28_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HEO6815926.1"
                     /translation="EDTIVHFMLSKGMIINAEKLENIKKFAQLSYGKNLGLYYISFKQ
                     RTEKEVIKYLQQHDIDSKIIPQIIDNLKSENWINDKNYVQSFIQQNLNTGDKGPYVIK
                     QKLLQKGIKSKIIESELQAINFQDLASKISQKLYKKYQNKLPLKALKDKLMQSLTTKG
                     FDYQIVHTVIQNLEIEKDQELEEDLIYKELDKQYQKLSKKHDQYELKQRIINALMRKG
                     YQYEDIKSALREYL"
     gene            785..1318
                     /locus_tag="VBC28_001985"
     CDS             785..1318
                     /locus_tag="VBC28_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002949762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HEO6815927.1"
                     /translation="MRLPKEGDFITIQSYKHDGSLHRTWRDTMVLKTTENALIGVNDH
                     TLVTENDGRRWVTREPAIVYFHKKYWFNIIAMIRETGVSYYCNLASPYILDPEALKYI
                     DYDLDVKVFADGEKRLLDVDEYEQHKAQMNYPTDIDYILKENVKILVEWINENKGPFS
                     SSYINIWYKRYLELKKR"
BASE COUNT          596 a          211 c          217 g          405 t
ORIGIN      
        1 cagaagatac tatcgttcat tttatgttaa gtaaagggat gataattaat gccgagaaac
       61 ttgaaaatat taaaaaattt gcccaactat catacggtaa aaatctaggt ctttattata
      121 tttctttcaa gcagagaacc gagaaagaag ttattaaata tcttcaacaa catgatattg
      181 attctaaaat tattccacaa attattgata atctaaaaag tgagaattgg ataaacgata
      241 aaaactacgt gcaatctttc atacagcaaa atctaaatac tggcgataag ggaccatacg
      301 tgataaaaca aaaattattg caaaaaggta tcaaaagtaa aataattgaa tctgaacttc
      361 aagctataaa ctttcaagac ttagcatcca aaataagcca aaaactctat aaaaaatatc
      421 aaaacaaact tccactaaaa gcattaaaag ataaattgat gcagagctta actacaaaag
      481 gatttgatta tcagatagtg cataccgtaa ttcaaaacct tgaaattgaa aaagatcaag
      541 aacttgaaga agacctcatt tataaagaat tagataaaca ataccaaaaa ttatcaaaaa
      601 aacatgacca gtatgaatta aagcaacgta taatcaatgc cttaatgcgt aaaggatacc
      661 agtacgaaga tatcaaaagt gctttaagag aatatttata agataataac tctcagacga
      721 tgtattttac agaaaattat gataaactat aacagacgta taaattgtag aaagttggta
      781 ggctatgaga ttacctaaag aaggcgactt tattacaatt caaagttaca aacatgatgg
      841 tagtttacac cgaacttggc gtgacaccat ggtattaaaa acaaccgaaa atgccctcat
      901 tggtgttaat gatcatactt tagtaacaga aaatgatggt cgacgctggg tgacacgaga
      961 gcctgcaata gtatactttc ataaaaaata ctggtttaac attatcgcta tgatacgtga
     1021 aactggtgtc tcctactatt gtaatctagc aagtccgtat atcttggacc ctgaagcact
     1081 caagtatatt gactatgacc ttgatgtcaa agtatttgca gatggtgaaa aaagactact
     1141 agatgtggac gaatatgaac agcataaagc tcagatgaac tatcctaccg atattgatta
     1201 tatattaaag gaaaatgtaa aaatattggt agaatggata aatgagaata aaggcccctt
     1261 ttcatcatca tatatcaata tctggtataa acggtacctt gaattgaaaa agcgctaact
     1321 aacacactaa atagtgtgtt tttattaata tcaaatttaa ttacaatact attgcaaaaa
     1381 tatatactta aaataaaaaa agtagaaaga tcactttcta cttttttaa
//
LOCUS       DATRMA010000145         1357 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.149, whole genome shotgun sequence.
ACCESSION   DATRMA010000145 DATRMA010000000
VERSION     DATRMA010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1357
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.149"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            <1..722
                     /locus_tag="VBC28_001986"
     CDS             <1..722
                     /locus_tag="VBC28_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEO6815928.1"
                     /translation="VTFTEERIDVRWGHYSQILAMLVLLKYMKSKEKYHYISIISESD
                     IPLKTGKEINTFLTLHKGKEFIGMVSNEQMSDFEYDKLKYNYPDFCFSKRGIFKKIYR
                     GTKVYRFFKNARYYSLPPLRKGSQWFTISREFTDWIIGYLDKHPDYQEAFCHSYCGDE
                     LFFQTLLAISPFQERVMNNKDDCLMGGRYIIWEADKTSPCDLFAEKVLEYRKEAESAL
                     FFRKVSEESDFDVYMHLLNKL"
     gene            738..>1357
                     /locus_tag="VBC28_001987"
     CDS             738..>1357
                     /locus_tag="VBC28_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6815929.1"
                     /translation="MKLLFIVPYFGKFNSYFQLFLNSIATSDLCDLLIVTDDKSKYDY
                     PQNVTIRYTSFEEFRNEVQRKMDFKITLDTPYKLCDFKPTYGYLFEDELLNYQYWGYC
                     DLDIIFGDLDGFLHPILNKGFDKIFELGHCTIIKNESRINRMFLSTVNGVKIGQKALS
                     SSKIEVFDEAYVNSINNIFIENNCNNFLYSLGADIDIWKNNFYITS"
BASE COUNT          476 a          160 c          230 g          491 t
ORIGIN      
        1 ttgtaacttt cacagaagaa agaatagatg ttcgttgggg acactattct cagattttag
       61 ctatgttagt tcttttaaaa tatatgaagt ccaaagaaaa atatcattat atttctatta
      121 ttagtgaaag cgacatccca ttaaaaacag gtaaagaaat taatactttt ttaacccttc
      181 ataaaggtaa agaattcata ggtatggttt ctaacgagca aatgagtgat tttgagtatg
      241 ataaactcaa gtataactat cctgattttt gtttttctaa aagaggaata tttaaaaaaa
      301 tttatagagg taccaaagtt tataggttct ttaaaaatgc aaggtattat tccctacctc
      361 ctcttcgtaa gggaagtcag tggtttacta tttcaagaga gtttactgac tggattattg
      421 gatatttaga taaacaccca gattatcagg aggctttttg tcatagctat tgtggagatg
      481 aattattttt tcaaacatta ctagctatct ctccatttca agaaagggtg atgaataata
      541 aagatgactg tctcatggga gggagatata ttatttggga ggctgataag acatcacctt
      601 gcgatttatt tgctgaaaaa gtgttagaat ataggaaaga agctgagagt gctttattct
      661 ttagaaaagt atctgaagaa agcgattttg atgtttatat gcatctatta aataaacttt
      721 aaattaagga gaaaatattg aaacttttat ttattgttcc atattttgga aaatttaata
      781 gttattttca gttattttta aactcaatag ccacatcaga tctttgtgat ttattgattg
      841 ttaccgatga taaaagtaaa tacgattacc ctcagaatgt tactattcgc tatactagtt
      901 ttgaagagtt ccgaaatgaa gtccaaagaa aaatggattt taaaattact ttagatactc
      961 catacaaact ctgtgatttt aaaccaacat acggttatct atttgaagat gaattgttaa
     1021 attatcaata ttggggttat tgtgacttag atattatctt tggtgattta gatggatttt
     1081 tacatcccat actaaataaa ggctttgata aaatttttga gctaggtcat tgtacgatta
     1141 ttaagaatga atcacgaata aatcgaatgt tcctttctac agttaatggg gtgaaaatag
     1201 gacaaaaagc tctaagttct tccaaaatcg aagtttttga tgaggcatat gttaatagta
     1261 ttaataatat atttattgaa aataattgta ataacttttt gtacagttta ggagcagaca
     1321 tagacatttg gaagaataat ttttatatta cttcttt
//
LOCUS       DATRMA010000146         1345 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.150, whole genome shotgun sequence.
ACCESSION   DATRMA010000146 DATRMA010000000
VERSION     DATRMA010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1345
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.150"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            <1..1217
                     /locus_tag="VBC28_001988"
     CDS             <1..1217
                     /locus_tag="VBC28_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000687011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEO6815930.1"
                     /translation="ILSILGLIVIYSTTSATLIQLGANPFRSVINQGVFWAVSLVAII
                     FIYKLKLNFLKNSKVLTMAVLVEVFLLLIARFFTQEVNGAHGWIVIGPISFQPAEYLK
                     VIIVWYLAFTFARRQKKIEIYDYQALTKGRWLPRSLSDLKDWRFYSLFMIGLVIAQPD
                     LGNGSIIVLTVIIMYCISGIGYRWFSALLGLIVVGSTLFIGTIAVVGVETMAKVPVFG
                     YVAKRFNAFFDPFKDLTDSGHQLANSYYAMSNGGWFGRGLGNSIEKLGYLPEATTDFV
                     FSIVIEELGVIGAGFILALVFFLILRIMHVGIKAKDPFNSMIALGIGAMLLMQVFVNI
                     GGISGLIPSTGVTFPFLSQGGNSLLVLSVAIGFVLNIDANEKKELIMKEAEEQYKPQE
                     KNEKIINLDAFK"
BASE COUNT          404 a          191 c          268 g          482 t
ORIGIN      
        1 tgatattgtc tattctaggc ttaattgtaa tttattcgac aactagtgca actctaattc
       61 agcttggagc taaccctttt cgttcagtaa tcaaccaggg tgtcttttgg gcagtcagtt
      121 tggttgcaat tatctttatc tataaactga aactaaattt tttgaaaaat tctaaagtgt
      181 tgactatggc agtattggta gaagtatttt tactactaat agcgcgcttt tttacacaag
      241 aagttaatgg agcacatggt tggattgtta ttggtcctat tagttttcaa cctgctgaat
      301 atttaaaagt tatcattgta tggtatttag cgtttacttt tgctcgaagg caaaaaaaga
      361 tagaaatata tgattatcaa gctttgacaa aagggagatg gcttccgaga tcactaagcg
      421 acctaaaaga ttggcgattt tattctttat ttatgattgg tttggtaatt gcacaacctg
      481 atttaggaaa tggttcgata attgttctaa cggtcattat tatgtactgt attagtggta
      541 ttggttatcg atggttttca gctttacttg gtctaatcgt cgtggggtca actttattta
      601 tcggaactat tgcagttgtt ggagttgaga caatggctaa agtccctgtc tttggttatg
      661 tagctaagcg atttaatgct ttttttgacc catttaaaga tttaacagat tcaggacacc
      721 agttggctaa ttcgtattat gcaatgagca acggtggctg gtttggtcga ggacttggga
      781 attctattga aaaattaggg tacttaccag aagcaacaac agactttgtc ttttcaattg
      841 ttattgagga attaggagtt attggtgcag ggtttatttt agctttggtt ttcttcttaa
      901 ttttacgtat tatgcacgta ggtataaagg ctaaagatcc tttcaactca atgatcgctc
      961 ttggtattgg agcaatgcta ttgatgcaag tttttgttaa cattggtggt atttctgggt
     1021 taatcccctc aactggagtg acatttcctt tcttatccca aggagggaac agcctcttgg
     1081 tactttctgt agctatcgga ttcgtgctta atattgatgc taacgagaaa aaggaactca
     1141 taatgaaaga agcagaagag caatataagc cacaagaaaa aaatgaaaaa ataatcaacc
     1201 ttgatgcctt taaataatca tccaaacaaa aatcgagatt tttatctcga tttttgtatt
     1261 ttttgaaatt taaactctaa ttaatagaga gaagatattt gaagaaaaga agtgtctatt
     1321 aatattctaa tactataaat tagga
//
LOCUS       DATRMA010000147         1321 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.151, whole genome shotgun sequence.
ACCESSION   DATRMA010000147 DATRMA010000000
VERSION     DATRMA010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1321
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.151"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            <1..391
                     /locus_tag="VBC28_001989"
     CDS             <1..391
                     /locus_tag="VBC28_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HEO6815931.1"
                     /translation="YKLFATVFTLIGFLIANLGLNAVITFSVPVLTLLYPIVIVIVLI
                     ILINKWLPLSKKGMSLTIGLVTLVSFVEVLAGQWQEKTLTQLVGFLPFHTISMGWLVP
                     MLIGIVFSLVLSDKQKGQAFDLEKFEG"
     gene            756..>1321
                     /locus_tag="VBC28_001990"
     CDS             756..>1321
                     /locus_tag="VBC28_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001006716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HEO6815932.1"
                     /translation="MNEVLRQEKKYLISLEDYYLLSKKFSQVMTLDSHSSDDGYTIRS
                     LYFDSLEDVDWQEKEDGVEIRRKIRLRNYGSQSNFAKLEMKQKQGANQKKRSLKLSKT
                     DAQALINGNNSVLLNYPDTFAAECYSRITTQLYQPKTVIEYQRKAFVAKENNIRITFD
                     HHIIGTEASYDIFSEDLNQNYLLDPYLV"
BASE COUNT          443 a          167 c          223 g          488 t
ORIGIN      
        1 ttataaatta tttgctactg tttttacttt gattggcttt ttaattgcta atcttggttt
       61 aaatgctgta attacttttt cagttcctgt gctaacttta ttgtatccga ttgttattgt
      121 tattgtctta attattttga ttaataagtg gttgccgctt tcaaaaaagg gaatgtcttt
      181 gacaattggt cttgtcactt tagtgtcgtt tgttgaggtt ctagcgggac aatggcaaga
      241 aaaaacatta actcagttag ttggattcct cccctttcat actatttcaa tggggtggct
      301 agttccaatg ttgattggta tagtattttc tttggtgctg tcagataaac aaaaaggcca
      361 agcatttgat ttagagaaat ttgaaggata gcactatttc aattttatag atatgtcaaa
      421 aaggttaaaa cttagtttta gcctttttta tatcagattt ttttaaatga tatatagatt
      481 gtagtaaggt gtgattaaac taagatataa acacttttta aaaattattt tataacagct
      541 gtggattgaa aatatacatt tttcttctag gtttaaatga gcgtaacaat catatttata
      601 gtataaaaat aaagtttgaa atgccttcgt ataaaaaaat atacattata tattttcctg
      661 ttgacaaggt gtataatttc tgatatattt atttttgctt aatttattgt taatatttat
      721 atataaatta atatttaatt aaacataggt gagtgatgaa cgaagttctt agacaggaaa
      781 aaaaatattt aatatcactt gaagattact atcttttatc taagaaattt tcccaagtaa
      841 tgacgctcga ttcgcatagt tcagacgacg gctatactat ccgttcgcta tactttgatt
      901 ctttagaaga tgttgattgg caagaaaagg aagatggtgt tgagatacga agaaagattc
      961 gtttgcgcaa ttatggtagt caaagcaatt ttgcgaaatt agaaatgaaa caaaaacaag
     1021 gtgctaatca aaaaaagcgc tctctaaaat taagcaagac agatgctcaa gcgctgatta
     1081 atggtaacaa tagtgtttta ttaaattatc cggatacatt tgctgcggag tgttattcaa
     1141 ggataacaac gcaactttat caacctaaaa cggttattga gtaccaaaga aaagcttttg
     1201 ttgctaagga aaacaatatc cgaatcacct ttgaccacca tataataggt acagaggcta
     1261 gttatgatat tttctcagag gatttaaatc aaaattattt attagatcct tatcttgtta
     1321 t
//
LOCUS       DATRMA010000148         1311 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.152, whole genome shotgun sequence.
ACCESSION   DATRMA010000148 DATRMA010000000
VERSION     DATRMA010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1311
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.152"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            complement(<1..1094)
                     /locus_tag="VBC28_001991"
     CDS             complement(<1..1094)
                     /locus_tag="VBC28_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEO6815933.1"
                     /translation="MHIIIQITIILLASVLATLISKRIGIPAVVGQLLVGIIIGPAML
                     GLVHQNQVLHVLSEIGVILLMFLAGLEANFDLLKKYLKPSLLVAITGVIVPMALFYFL
                     TRLFGFQINTAIFYGLVFAATSISITVEVLQEYNRVKTDTGAIILGAAVADDVLAVLL
                     LSVFIATNGSSSNIGLQIIIQLLFFVFLFICMKYLVPALFKLIEKVHFFEKYTILAIL
                     ICFSLSILADKVGMSSIIGSFFAGLAIGQTSFVDKVEHKISLLSYTFFIPIFFASIAL
                     PLKFDGMMSHLHTILIFTALAVLSKLIPGYFVGRGFNFSKLESLTIGGGMVSRGEMAL
                     IIVQVGLAAKIISSTTYSELVIVVILSTIIA"
BASE COUNT          523 a          212 c          210 g          366 t
ORIGIN      
        1 gcaataatag ttgatagtat aacaacaata acaagctcgg aataagtagt gcttgaaata
       61 attttagcag ctaatccaac ttgaactata atgagagcca tctcgccacg actgaccatt
      121 ccaccaccaa ttgttaaaga ttctaatttt gaaaaattaa agcctcgacc gacaaagtac
      181 cctggaatta attttgataa aacagccaaa gcagtaaaga ttaatatcgt gtgaaggtga
      241 gacatcattc catcgaattt aagaggtagt gcaattgaag cgaagaaaat tgggataaag
      301 aaggtgtaac taagaagtga aattttatgt tctaccttat caacaaaaga agtttgtcca
      361 atagctaatc ctgcaaaaaa cgaaccaatg atagaactca ttccaacttt atcggctaaa
      421 attgatagag aaaaacagat aaggatagca agaatagtat atttctcaaa gaaatgaacc
      481 ttttcgatta atttgaaaag agcaggtaca aggtatttca tacaaataaa taagaaaaca
      541 aagaataaca attgaatgat aatttgtaat ccgatattag aagagcttcc atttgttgca
      601 ataaagacac tcagaagcaa aacagctaag acatcatcag caacagcagc acctaaaata
      661 atagcaccag tatctgtttt tacacgattg tattcttgta aaacttcaac tgtgatagaa
      721 atactagttg cagcaaatac gagcccgtaa aagatagcag tattgatttg gaacccaaac
      781 aagcgtgtca agaagtagaa gagagccata ggaacaataa caccagtaat tgcgaccagt
      841 agactgggct taagatactt ctttaaaaga tcaaaattag cttcaagacc cgctaaaaac
      901 atgagtaaga taacaccaat ttctgacaag acatgtaata cctgattttg atgaacaagt
      961 cctagcattg caggtccgat gatgatacct acaagtagtt gtccaacaac agccggtatt
     1021 ccaattcttt ttgaaataag agttgcaaga acgcttgcca acaaaatgat tgtaatttga
     1081 atgataatat gcattttttc ctcctaatat taataaaatg atatcaatca tattgtactg
     1141 aatatttaga ataccatttt ttaagcctaa aatctaggaa tatttctttt ctgtcttttt
     1201 catattatta tcaagaaaat ggaaaaatat actgaaaaat attcaattta agaataatga
     1261 acttgtaact atcgaactac ataaaaatga tattttaagt gtaagtatct c
//
LOCUS       DATRMA010000149         1215 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.153, whole genome shotgun sequence.
ACCESSION   DATRMA010000149 DATRMA010000000
VERSION     DATRMA010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.153"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            31..>1215
                     /locus_tag="VBC28_001992"
     CDS             31..>1215
                     /locus_tag="VBC28_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HEO6815934.1"
                     /translation="MNLQKNDKTNSFIHYANGPSLEEINGTIEVPKDLSFFKTLLAYS
                     GPGALVAVGYMDPGNWSTSITGGQNFQYLLISIILMSSLIAMLLQYMSAKLGIVTQMD
                     LAQAIRARTSKQLGIVLWILTELAIMATDIAEVIGGAIALYLLFHIPLAIAVFITVFD
                     VLLLLLLTKIGFRKIEALVVALILVIFLVFAYQVALSHPIWTDIFKGLVPTSEAFSTS
                     HTVNGQTPLSGALGIIGATVMPHNLYLHSSVVQSRKLDHNNKKDIARAIRFSTFDSNI
                     QLTVAFFVNSLLLIMGVAVFKTGSVTDPSFFGLFKALSNSTIMSNSILAHIASSGILS
                     LLFAIALLASGQNSTITGTLTGQIIMEGFIHMKVPIWFRRIITRLISVIPVMICVLVT
                     SGR"
BASE COUNT          358 a          200 c          201 g          456 t
ORIGIN      
        1 tactatttca atggaaaggt ttatagttca atgaatctac aaaaaaatga taaaacaaat
       61 agttttatcc attatgcaaa cggtccttcc ctagaagaaa taaatgggac aattgaagtt
      121 cctaaagatt tgagcttttt taaaacttta ctagcttatt ctggaccggg tgcacttgtt
      181 gctgttggtt atatggatcc tggaaattgg tccacttcca taactggcgg tcaaaatttt
      241 caatatttac taatttcaat tatcttaatg tctagtttaa ttgctatgct acttcaatac
      301 atgtcagcta agctaggtat tgttactcag atggatcttg ctcaagctat ccgagcaagg
      361 accagtaaac agttgggaat tgtcttatgg attctcactg agcttgctat aatggcaaca
      421 gatattgctg aagttattgg tggggctatt gcactttatt tactgtttca tattccactt
      481 gcgattgctg ttttcattac agtatttgat gtattactct tattattgtt gacaaaaata
      541 ggatttcgaa aaattgaagc tctagtagtt gctttaatat tggttatttt cttagttttt
      601 gcatatcaag tggctctttc tcatcctata tggacggata tttttaaagg tttagtacct
      661 acatctgaag ctttctcaac ctctcataca gtaaatggac aaacaccact ttcaggtgcc
      721 ttaggcatta ttggggcaac tgttatgcca cataatcttt atctacattc ttcagttgtt
      781 caaagtcgaa aactagatca taataacaaa aaagatatcg ctagagcaat taggttttca
      841 acttttgatt caaatattca attaacagta gccttttttg ttaattcact attactcatt
      901 atgggcgttg ctgttttcaa aacaggaagt gttactgatc catcattctt tggtctattt
      961 aaagcactat ctaattctac tattatgagt aattctattt tagctcatat agcaagttca
     1021 ggtattttat cacttttatt tgcaatagct ttattagcat ctggacagaa ttcaactatt
     1081 actggaacat taacaggtca aattattatg gaaggattta ttcatatgaa agttccaatt
     1141 tggttccgtc gtatcattac tcgcctaatt tctgttattc ctgtaatgat ttgtgtctta
     1201 gtaactagtg gtaga
//
LOCUS       DATRMA010000150         1144 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.154, whole genome shotgun sequence.
ACCESSION   DATRMA010000150 DATRMA010000000
VERSION     DATRMA010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1144
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.154"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            complement(<1..>1144)
                     /locus_tag="VBC28_001993"
     CDS             complement(<1..>1144)
                     /locus_tag="VBC28_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO6815935.1"
                     /translation="DTPSLSLDTACNRLHLSPVELKNYCHKLNVLFGHKLSIHIQKKR
                     ILCKLNKLTQEDALKIVYNESNTLQLLKFLIDDSSDKKSLVAFGNDLFLSRSSVYRLR
                     ENLIPLIKDLGLSLSKNTIVGDEYRIRYLIAYLTTKFGIKIYDLSALDIKIIRQFLFE
                     SGTNIRPTTHLSDIFVFFDTLFILTWKRHHHKVKIPNSKLFSSLKKIFIYEELNTCVK
                     SIVQPYFNVIFNSEDMDYIFLIYLTSANSFAAQKWTETHVDEVLAIFKSLPKFQMLLQ
                     PLKEALPYSDTYYDELVKVTIYFSRNFIVGLNQLIPETLSFPSTFYPGNNPKLISIIE
                     PIVTHWLTEIGESKFKKTHFFLLCAHLERVIKNSMPPINIVLLTTDF"
BASE COUNT          408 a          145 c          220 g          371 t
ORIGIN      
        1 taaaatcagt tgtcagcaac acaatattta taggaggcat gctattcttt attactcttt
       61 caaggtgagc acagagcaag aagaagtgcg tttttttgaa ctttgattct ccaatttcag
      121 ttagccaatg agtgactatt ggttcgataa tggatattag tttaggatta tttccaggat
      181 agaatgttga aggaaaacta agtgtttcag gaattaattg attcagacca actatgaagt
      241 ttcgagaaaa gtaaatagtg actttcacta gctcatcgta gtaggtgtca gagtatggaa
      301 gagcttcttt caaaggttgt agtagcattt ggaatttagg aagactttta aaaatagcta
      361 acacttcatc gacatgtgtt tcagtccact tttgagcagc aaaagagtta gcgcttgtaa
      421 gatagataag aaaaatataa tccatgtctt ctgaattaaa aataacattg aagtaaggtt
      481 gaacgatact ttttacacat gtattcagct cctcataaat aaagattttt ttaagagaag
      541 agaaaagttt ggagtttgga attttaactt tatgatggtg acgtttccag gttaaaatga
      601 aaagcgtatc aaaaaaaaca aagatatcag aaaggtgcgt agtaggtctt atattagtac
      661 cactctcaaa taaaaattgt cgaataatct taatatctag agcagacaga tcgtaaattt
      721 taataccaaa tttagtagtg aggtatgcga tgagatatct aatgcgatat tcgtcaccta
      781 caatggtatt tttagataaa gataatccta aatctttaat taaaggaatt aaattttctc
      841 gcagacgata gacagaagaa cgagataaga agagatcatt tccaaaagct actagagatt
      901 ttttatcaga cgagtcatca attaaaaatt taagaagttg taaagtgttt gactcattat
      961 aaactatttt taaggcatct tcctgagtaa gtttatttag cttacataaa atccgttttt
     1021 tttggatatg gatagaaagt ttatgaccaa ataaaacatt taatttatga caatagtttt
     1081 taagctcaac tggtgataag tggagtcggt tacaggcggt atctaaggat agtgaggggg
     1141 tatc
//
LOCUS       DATRMA010000151         1114 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.155, whole genome shotgun sequence.
ACCESSION   DATRMA010000151 DATRMA010000000
VERSION     DATRMA010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1114
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.155"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            <1..483
                     /locus_tag="VBC28_001994"
     CDS             <1..483
                     /locus_tag="VBC28_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HEO6815936.1"
                     /translation="WVIIDYIEFGDLLTFIKNLDKSLQNDVAIDLVSFVLDNLKITQQ
                     FTPETMLGLLNNIRETRNVCAHNNRLIGFECRASSTYYSDLHSLFNITPDDSRKNVFT
                     TFISLQCFLSNSEYAKLHNSIRKRVNQLKNKVGQDKVNEILEQLGFPINWNDQPKLRQ
                     "
     gene            602..>1114
                     /locus_tag="VBC28_001995"
     CDS             602..>1114
                     /locus_tag="VBC28_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
                     /protein_id="HEO6815937.1"
                     /translation="MAYFRKRDNGWEYRISYKDITGKYRQKSKSGFKTKKQAELEANK
                     IEIELSKGFQINNNIEFCTYFKNRSELYKKPNVTATTWDKYNYTLSKIKGYFDKAKLS
                     SIKPSHYQKFISDYLNQYNWQTIKMINTHIRQSIKMAIHEGYITKDFTAFVVLPKPDD
                     SNVDSKFLELS"
BASE COUNT          444 a          159 c          177 g          334 t
ORIGIN      
        1 tgggtaatta ttgattatat agagtttgga gatttgttaa cttttataaa aaatcttgat
       61 aaaagtttgc aaaatgatgt tgctattgat ttagtaagtt ttgtacttga taatctaaaa
      121 atcacacaac aattcactcc tgaaacaatg ctaggtttat taaataatat aagagagact
      181 aggaatgttt gtgcccataa caataggcta ataggatttg aatgtagagc tagctctaca
      241 tactattctg acttacacag tctgtttaat ataacacctg acgattcaag aaaaaatgta
      301 ttcactacat ttattagttt acaatgcttt cttagtaact cagaatatgc aaaattgcat
      361 aactcaataa gaaaaagagt aaatcaatta aaaaataaag ttgggcaaga taaagttaat
      421 gaaattcttg aacaacttgg attcccaata aattggaatg accaaccaaa acttagacaa
      481 taaaaaaagc cccacgctca aattttggtc aaggagagcg tgaggcaaaa ctgtataaga
      541 aataagcatt aaatggctcg ttttcttgta cctattttag caaatttaga aaggtttagc
      601 aatggcgtat ttccgtaaac gcgacaacgg gtgggagtat cgaatatcgt ataaagatat
      661 aactggtaag taccgacaaa aatcaaagag cggttttaaa acaaagaaac aagctgaact
      721 tgaagcaaat aagatagaga tagaattatc taaaggattt caaataaata ataatataga
      781 attttgtact tactttaaaa atcggtctga actttataaa aagccaaatg taactgctac
      841 tacttgggat aagtacaact atactttgtc taagataaaa gggtatttcg ataaagccaa
      901 gttatcatca attaaaccaa gtcattacca aaaatttatt agtgattatt taaaccagta
      961 caactggcaa actattaaaa tgataaatac tcacatcaga caatcaataa aaatggctat
     1021 ccatgaaggt tatattacta aagatttcac tgctttcgtc gtccttccga aaccagatga
     1081 tagtaacgta gatagtaaat ttttagagct atca
//
LOCUS       DATRMA010000152         1073 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.156, whole genome shotgun sequence.
ACCESSION   DATRMA010000152 DATRMA010000000
VERSION     DATRMA010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.156"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            <1..935
                     /locus_tag="VBC28_001996"
     CDS             <1..935
                     /locus_tag="VBC28_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000160532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30-like element ISSag9 family transposase"
                     /protein_id="HEO6815938.1"
                     /translation="CGYKKTFYLAKQAQRNYEKLLVESREGIPLNKETFWKIDRVLSN
                     GVKKGQRIYHILKTNDLEVSSSTVYRHIKKGYLSITPIDLPRAVKFKKRRKSTLPPIP
                     KAIKEGRRYEDFIEHMNQSELNSWLEMDTVIGRIGGKVLLTFNVAFCNFIFAKLMDSK
                     TAIETAKHIQVIKRTLYDNKRDFFELFPVILTDNGGEFARVDDIEIDVCGQSQLFFCD
                     PNRSDQKARIEKNHTLVRDILPKGTSFDNLTQEDINLALSHINSVKRQALNGKTAYEL
                     FSFTYGKDIASILGIEEITAEDVCQSPKLLKDKI"
BASE COUNT          354 a          232 c          206 g          281 t
ORIGIN      
        1 actgtggtta caagaagacc ttctatcttg ctaagcaagc tcaaagaaac tatgaaaaac
       61 ttttagttga atctagagaa ggaatccctt tgaacaaaga gaccttctgg aaaatagata
      121 gagtgctttc taatggggtc aagaagggcc aacgtatcta tcatatcctc aaaaccaacg
      181 atctagaagt gagttcttca accgtttatc gacacatcaa gaaaggctac ctatccatca
      241 caccaatcga cctacccaga gctgtgaagt tcaaaaaaag gcgaaagagc acactccctc
      301 ctattccaaa agcgattaaa gaagggcgac ggtacgagga ttttatagaa cacatgaacc
      361 aatcagagct gaactcctgg cttgaaatgg acacggttat tggacggatt ggtggaaagg
      421 tccttctcac cttcaatgtc gccttctgta acttcatctt tgccaaactg atggattcta
      481 agacagctat cgaaactgct aaacacatac aggtcattaa gaggacactc tatgataaca
      541 agagagactt cttcgaactc tttcctgtta tcctaacgga taatggtggg gaattcgcta
      601 gagtggatga tattgagata gatgtttgtg gacagtctca actcttcttt tgtgacccta
      661 atcggtctga ccagaaagca agaatcgaga agaatcatac cctcgtgaga gacatactgc
      721 ctaaggggac ttccttcgac aacttgactc aagaggacat taatctggca ctatctcata
      781 tcaacagcgt taagagacag gctctaaatg gtaaaacggc ttatgagcta ttctctttta
      841 cttacggaaa agacatcgca agtatactcg gtattgagga aattactgcg gaagacgtct
      901 gccaatctcc caaactatta aaagacaaaa tctaacctaa tcaactaact gatacaacct
      961 aaacgtctca cactaacttc aaccatagcg gaacttaatc tgaaacgctt tcagatgagg
     1021 atgttttctt gtaccctttt tttgcgattt ttaactctga aaacctatga aat
//
LOCUS       DATRMA010000153          877 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.160, whole genome shotgun sequence.
ACCESSION   DATRMA010000153 DATRMA010000000
VERSION     DATRMA010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..877
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.160"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            144..848
                     /locus_tag="VBC28_001997"
     CDS             144..848
                     /locus_tag="VBC28_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000159984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B3/4 domain-containing protein"
                     /protein_id="HEO6815939.1"
                     /translation="MTKFIVDSSYWNLFPTSKIGVILIKDYHMDRNLETELKQLLSDS
                     HSLAKKYLQEKEFSQNRVIQTYRKAYQTFKTKKGARSSIEALLKRVNSGNEITSINPL
                     VDIYNAASLRFGLPIGAEDSDTFRGDLKLTITNGGDEFYLIGEDFNRPTLSGELAYVD
                     DVGAVCRCFNWRDGKRTMITDNTQNAFLVIELIDNGREIIFKEALDFIATNTNRFLKA
                     KTQTIILDKEHSEITL"
BASE COUNT          316 a          129 c          149 g          283 t
ORIGIN      
        1 tgtataaaat tttcttgtca cgataaatcc tccttcatta tagcaaaaaa attcgtagaa
       61 agaacgtttt tcgttgaatt ttgaaaacgc ttttgagaaa atgtaattgt tctgatatct
      121 tttaaaaaaa ttgagaggcc ttcatgacta aatttattgt tgattcatcg tactggaatt
      181 tgtttccaac ttctaaaatt ggagtaatct taatcaaaga ttatcacatg gacagaaacc
      241 ttgaaacaga acttaaacag ttactgtctg atagtcattc tttagcaaaa aaatatttgc
      301 aagagaaaga attcagtcaa aacagagtta tccaaactta tcgcaaagca tatcaaacct
      361 ttaaaactaa aaaaggtgca cgttcaagta tcgaagcatt attgaagcga gttaattcag
      421 gaaacgaaat tactagtata aatcctctag ttgatatcta taatgccgct agccttaggt
      481 ttggattacc tattggtgct gaggatagtg atacatttag aggtgattta aaactaacta
      541 ttaccaatgg tggtgatgaa ttttacttga ttggcgaaga ttttaacaga ccaactcttt
      601 caggtgagct tgcctatgtt gatgatgttg gagcagtttg ccgctgtttt aattggcgtg
      661 atggaaaacg tactatgatt accgataata ctcagaatgc ttttttagtt atcgaattaa
      721 ttgataatgg aagagaaatt atttttaaag aagcgttgga ctttatcgcc accaatacaa
      781 atcgttttct aaaagctaaa acacagacca tcatattaga taaagaacac agtgaaataa
      841 cgctataaca ttaaaacgaa aagtaaaaat tttactt
//
LOCUS       DATRMA010000154          817 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.161, whole genome shotgun sequence.
ACCESSION   DATRMA010000154 DATRMA010000000
VERSION     DATRMA010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..817
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.161"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            18..>817
                     /locus_tag="VBC28_001998"
     CDS             join(18..392,395..>817)
                     /locus_tag="VBC28_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEO6815940.1"
                     /translation="MNYEASKQLTDVRFKRLVGVQRTTFEEMLAVLKTAYQRKHAKGG
                     RTPKLSLEDLLMATLQYMREYRTYEQIAADFGIHESNLIRRSQWVESTLIQSGFTISK
                     THLSAEDTVIVDATEVKINRPKKQLANYSGKKKCHAMKAQAIVTSQGRIVSLDIAVNY
                     CHDMKLFKMSRRNIGQAAKILADSGYQGIMKMYSQAQTPRKSSKLKPLTLEDKTYNHT
                     LSKERIKVENIFAKVKTFKIFSTTYRNRRKRFGLRMNLIAGMINRELG"
BASE COUNT          286 a          147 c          167 g          217 t
ORIGIN      
        1 tatggtacaa tagttctatg aattatgaag caagcaaaca attaactgat gtacgattta
       61 agcgccttgt tggtgttcag cgcactactt ttgaagagat gttagctgtg ttaaaaacag
      121 cttatcaacg taaacacgca aaaggtggcc gaacccctaa gttaagctta gaagatctcc
      181 tcatggctac tcttcaatac atgcgagaat accgcactta tgaacaaatt gcggctgatt
      241 ttggcattca cgaaagcaac ttaatccgtc gaagtcaatg ggttgaatca actcttattc
      301 aaagtggttt tacgatttca aaaactcatc ttagtgctga ggatacggtg attgtggatg
      361 caacagaggt aaaaatcaat cgtcctaaaa aaatcaacta gcgaattatt ctggtaaaaa
      421 gaaatgtcat gctatgaagg ctcaggcgat tgtcacaagc caagggagaa ttgtttcttt
      481 ggatattgca gtgaactatt gccacgatat gaagttgttc aaaatgagtc gcagaaacat
      541 cggacaagct gctaaaatct tggcagacag tggttatcaa gggatcatga agatgtattc
      601 acaagcgcaa actccgagga aatcaagcaa acttaagcca ctaactcttg aagataaaac
      661 ctataaccat acgctatcca aagagagaat caaggttgag aatatttttg ccaaagtaaa
      721 aacgtttaaa atattttcaa caacctatcg aaatcgacgc aaacggtttg gattacgaat
      781 gaatttgatt gctggaatga tcaaccgtga actagga
//
LOCUS       DATRMA010000155          805 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.162, whole genome shotgun sequence.
ACCESSION   DATRMA010000155 DATRMA010000000
VERSION     DATRMA010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..805
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.162"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            122..>805
                     /locus_tag="VBC28_001999"
     CDS             122..>805
                     /locus_tag="VBC28_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-like surface protein"
                     /protein_id="HEO6815941.1"
                     /translation="MFRRSKNNSYDTLQTKQRFSIKKFKFGAASVLIGISFLGGFTQG
                     QFNISTDTVFAAEVISGSAVTLNTNMTKNVQNGRAYIDLYDVKNGKIDPLQLITLNSP
                     DLKAQYVIRQGGNYFTQPSELTTVGAASINYTVLKTDGSPHTKPDGQVDIINVSLTIY
                     NSSALRDKIDEVKKKAEDPKWDEGSRDKVLISLDDIKTDIDNNPKTQSDIANKITEVT
                     NLEKILVPRI"
BASE COUNT          313 a          100 c          147 g          245 t
ORIGIN      
        1 gaaaaaaaaa aaaaaaaaca gattagatcg gataaaaaag tatagatatt ctaatatttg
       61 tttttaaagc ctatacttta ctatgtatag agctatacag aataaagtaa aggagaatat
      121 tatgtttaga aggtctaaaa ataacagtta tgatacttta cagacgaaac aacggttttc
      181 aattaagaag tttaagtttg gtgcagcttc tgtactaatt ggtattagtt ttttaggagg
      241 ttttactcaa gggcaattta atatttctac agatactgtg tttgcagctg aagtaatttc
      301 aggaagtgct gttacgttaa acacaaatat gactaaaaat gttcagaatg gtagagcata
      361 tatagattta tatgatgtga aaaatgggaa aatagatcca ttacaattaa ttacgttaaa
      421 ttcacctgat ttaaaagctc agtatgtcat taggcaaggc ggcaattatt tcacacaacc
      481 ttctgaattg actactgttg gtgcagctag tattaattat acagtattga agacagatgg
      541 aagtcctcat acgaagcctg atggacaagt ggatattata aacgtttcat tgactattta
      601 caattcttca gctttgagag ataaaataga tgaagttaaa aagaaagcgg aagaccctaa
      661 atgggacgag ggaagtcgcg ataaagtttt gataagttta gatgatatca aaacagatat
      721 tgataataat cctaagacgc aatcagacat tgccaataaa ataactgaag ttactaattt
      781 agaaaaaata ctagtacctc gaatc
//
LOCUS       DATRMA010000156          750 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.164, whole genome shotgun sequence.
ACCESSION   DATRMA010000156 DATRMA010000000
VERSION     DATRMA010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..750
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.164"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            complement(<1..479)
                     /locus_tag="VBC28_002000"
     CDS             complement(<1..479)
                     /locus_tag="VBC28_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001086432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HEO6815942.1"
                     /translation="MNSLDNLLSHNLEDNRHLLPYEHMHTEVRNGYPDILFHWHPELE
                     ISYVHEGTARYHIDYDFFNSQSGDIILIRPNGMHSIHPIENKEHITDSIKFHLDLIGY
                     SIVDQVSLRYLQPLQTSSFKFIQCIKPSMTGYNDIKNCLFDIFNISKEENRHFELLL"
BASE COUNT          261 a          102 c          122 g          265 t
ORIGIN      
        1 tttaggagta gctcaaaatg tcgattttct tcttttgaaa tattaaaaat atcaaacaaa
       61 caatttttga tatcattata tcctgtcata ctaggtttga tacattgaat aaacttaaaa
      121 gagctagtct gcaacggttg taaataacga aggctaactt ggtctacaat agaatatcct
      181 attaaatcta agtgaaattt aatgctatct gtgatatgct ctttattttc aatcggatga
      241 atagaatgca taccatttgg acggatcaag ataatatcac cagattggct attaaaaaaa
      301 tcataatcga tgtgataacg tgcggtcccc tcgtggacat atgaaatttc aagttccgga
      361 tgccagtgaa ataaaatgtc agggtaacca tttctaacct cagtatgcat gtgttcatag
      421 ggtaataaat gccggttatc ttctaaatta tgtgatagta aattatcaag tgagttcatc
      481 atttttcttt ctaaaatcgt aggatagtat tattataaca gaaaactatc aaaaaatggg
      541 tttcgtagga tagtattatt ttttgaaagg atagtgtaag ttttgtatag gaacatgaca
      601 tataatataa gtcatagaga aaacgcttta aaatttttgc ttagagtgtt tctaataact
      661 tcaaaatcct tacagggtcg tttaatctcc ttatctcatg taaggatttt ttgttgtatt
      721 aatatgacca aatttattaa tatactcaat
//
LOCUS       DATRMA010000157          746 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.165, whole genome shotgun sequence.
ACCESSION   DATRMA010000157 DATRMA010000000
VERSION     DATRMA010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..746
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.165"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            13..585
                     /locus_tag="VBC28_002001"
     CDS             13..585
                     /locus_tag="VBC28_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001231800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEO6815943.1"
                     /translation="MRKRSILPILAVLFVFMSIFSVYSIFRGDKAKEKASVIKQASQT
                     SQTSKKEVLQKKTYPNLNKYSNLEIHVSSTRQTMTITSNDKVIFKTIVSTGAKESPTP
                     KGTFVIEPERGDFFYNASSKEGAYYWVSFKEHGIYLFHSVPTDQQGNEIPEEAKQLGK
                     AASHGCVRMSRADAKWFYENIPQGTTVTIK"
BASE COUNT          280 a          128 c          130 g          208 t
ORIGIN      
        1 agaggatagt acatgagaaa acgttcaatt ttgccaatat tagctgtctt atttgttttc
       61 atgagtatct ttagcgtata ttctattttt cgtggtgata aagccaaaga aaaagcaagt
      121 gtcataaaac aagcatctca aacaagtcaa acttcaaaaa aagaggtctt acaaaagaag
      181 acttatccaa atctaaataa atattcaaat ctagaaattc atgtttcatc tacgaggcaa
      241 acaatgacaa taacctcgaa tgacaaggtc atctttaaaa cgattgtttc aacaggagca
      301 aaagagagcc ctaccccaaa aggaactttt gtcatagaac cggaacgagg agattttttc
      361 tataacgcct catcaaaaga aggagcctat tattgggtct cctttaaaga acatggtatt
      421 tatctctttc atagcgttcc gactgatcaa caaggaaatg agattccaga agaagcaaag
      481 caattaggaa aagccgcctc acatggctgt gtgcgtatgt caagggcgga tgcaaaatgg
      541 ttctatgaaa atattcctca aggaacgact gttaccatca aatagtctta atgtggtaaa
      601 aatcatgtta ttaaaagctc ggagtaaact tccagagctt tttcgtatat cccttaaact
      661 caattagtaa acgaaaccta tcagcggaat aaatgtataa agtatttcga tatcattaaa
      721 aaaatgatag aataatagtg ttaatc
//
LOCUS       DATRMA010000158          625 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.170, whole genome shotgun sequence.
ACCESSION   DATRMA010000158 DATRMA010000000
VERSION     DATRMA010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..625
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.170"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            <1..170
                     /locus_tag="VBC28_002002"
     CDS             <1..170
                     /locus_tag="VBC28_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014619950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4297 domain-containing protein"
                     /protein_id="HEO6815944.1"
                     /translation="ISFFKSDEDRLENEEMTILIPKSFDLSEMHTLSENSKYAIGISA
                     YCDILEGIANE"
     gene            163..>625
                     /locus_tag="VBC28_002003"
     CDS             163..>625
                     /locus_tag="VBC28_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HEO6815945.1"
                     /translation="MNKLVIKSLLIVDYENKKANKFRFSDSANLIVSSTNGEGKSSLV
                     KSIYYALGANLKSFPKGWNNSDYIFQLEVSIDDKDFLIKRHNKVISISDNGRVQVFEN
                     FKEYSSWFQKKLGMNLELVSKNSDTPSLASVEALLSPMYIDQDKSWDGKLFK"
BASE COUNT          224 a           79 c          124 g          198 t
ORIGIN      
        1 ttattagttt ttttaaaagc gatgaagaca gattagaaaa cgaagaaatg actattttga
       61 tacccaaatc gtttgattta tctgaaatgc atacattatc ggagaatagt aagtatgcta
      121 ttgggatatc agcgtactgc gatattttgg aggggattgc taatgaataa actagtaata
      181 aaaagtcttt tgatagtaga ctatgaaaac aaaaaagcta ataaatttag attttctgat
      241 tcagcaaatc taatagtcag tagtacaaac ggcgagggaa agtctagtct agttaaatca
      301 atttattatg ctttgggagc caatcttaag tctttcccaa aaggttggaa caatagcgat
      361 tatatctttc agctggaagt ctctattgat gataaggatt ttttgataaa aagacacaat
      421 aaagttattt caatttcaga caatggaaga gttcaggtgt ttgaaaattt caaagaatat
      481 tcttcatggt ttcaaaaaaa gttgggcatg aatctggagt tggttagtaa aaatagcgac
      541 acccctagtt tagcatctgt tgaggctctt ctgagcccaa tgtatattga ccaagataaa
      601 tcatgggacg ggaaattatt taaag
//
LOCUS       DATRMA010000159          596 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.172, whole genome shotgun sequence.
ACCESSION   DATRMA010000159 DATRMA010000000
VERSION     DATRMA010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..596
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.172"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            complement(20..91)
                     /locus_tag="VBC28_002004"
     tRNA            complement(20..91)
                     /locus_tag="VBC28_002004"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(57..59),aa:Gln,seq:ttg)
     gene            complement(98..170)
                     /locus_tag="VBC28_002005"
     tRNA            complement(98..170)
                     /locus_tag="VBC28_002005"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(135..137),aa:His,seq:gtg)
     gene            complement(185..255)
                     /locus_tag="VBC28_002006"
     tRNA            complement(185..255)
                     /locus_tag="VBC28_002006"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(221..223),aa:Trp,seq:cca)
     gene            complement(262..342)
                     /locus_tag="VBC28_002007"
     tRNA            complement(262..342)
                     /locus_tag="VBC28_002007"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(306..308),aa:Tyr,seq:gta)
     gene            complement(354..426)
                     /locus_tag="VBC28_002008"
     tRNA            complement(354..426)
                     /locus_tag="VBC28_002008"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(391..393),aa:Phe,seq:gaa)
     gene            complement(429..502)
                     /locus_tag="VBC28_002009"
     tRNA            complement(429..502)
                     /locus_tag="VBC28_002009"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(466..468),aa:Met,seq:cat)
BASE COUNT          156 a          165 c          143 g          132 t
ORIGIN      
        1 cacgccggct atcttaaaac tggggtagct ggattcgaac caacgcatga gggagtcaaa
       61 gtcccttgcc ttaccgcttg gctatacccc atgaaaaggc gagtgatggg aatcgaaccc
      121 acgaatgtca gagccacaat ctgatgtgtt aaccacttca ccacacccgc catattagaa
      181 aaaacacggg cagtaggaat cgaacccaca ctgaaggttt tggagacctt agttctacct
      241 ttaaactatg cccgtttact atggagagag agggattcga acccccgaac ccgaaggagc
      301 ggatttacag tccgccgcgt ttagcctctt cgctatctct cctaaggtat aaatggcgcg
      361 agacggaatc gaaccgccga cacatggagc ttcaatccat tgctctacca actgagctac
      421 cgagcctatt gcgggagcag gatttgaacc tacgaccttc gggttatgag cccgacgagc
      481 tacctagctg ctccatcccg cgatatcttt aaaggaggat gtgggattcg aacccacgca
      541 cgcttttaca cgcctgacgg ttttcaagac cgttcccttc agccggactt gggtaa
//
LOCUS       DATRMA010000160          593 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.173, whole genome shotgun sequence.
ACCESSION   DATRMA010000160 DATRMA010000000
VERSION     DATRMA010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..593
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.173"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            <1..>593
                     /locus_tag="VBC28_002010"
     CDS             <1..>593
                     /locus_tag="VBC28_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6815946.1"
                     /translation="LLKRLLETNADIAETDFALIDNRFTKKKRKKIQKVLNKEEAIRE
                     FLSGNVVENNLVIKLFKKTVIANLKFKEDVIVGEDMLFSLQALQNSDRVTVDTTNADY
                     FYVVRSNSTMNTITEKDIDNLSILEQEFKKIDIPQLKSYLEAKLIREKVKFVSRVLIS
                     NKAHLYSDIIDRYLQEVRHYSIKNMINFLSSKHCLTIT"
BASE COUNT          232 a           72 c          104 g          185 t
ORIGIN      
        1 ttattaaaga gattattaga aactaatgcg gatatcgccg aaactgactt tgccttaatt
       61 gataatagat ttacgaaaaa aaagaggaaa aagatacaga aagttctgaa taaagaagaa
      121 gctattaggg agtttttatc aggaaatgtg gttgaaaata atctcgtcat aaagttattt
      181 aaaaaaacag ttattgctaa tctaaaattt aaagaagatg tgatagttgg tgaagatatg
      241 ctattttctt tgcaggcctt acaaaactca gatagagtta cagtggacac tacgaatgcg
      301 gattattttt atgtcgtacg ttcaaattca actatgaata ctattaccga gaaggatatc
      361 gataatcttt caatactcga gcaggagttc aaaaaaattg atatccccca attaaaaagt
      421 tatttggaag ccaaattaat tcgagaaaag gttaaatttg tatctagagt attaattagt
      481 aataaagcac atttgtacag tgatattatt gatagatatt tgcaagaggt tagacactac
      541 agcataaaaa atatgataaa ctttctttcg tcgaaacatt gtttgactat aac
//
LOCUS       DATRMA010000161          541 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.175, whole genome shotgun sequence.
ACCESSION   DATRMA010000161 DATRMA010000000
VERSION     DATRMA010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..541
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.175"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            complement(1..73)
                     /locus_tag="VBC28_002011"
     tRNA            complement(1..73)
                     /locus_tag="VBC28_002011"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(37..39),aa:Ile,seq:cat)
     gene            complement(93..166)
                     /locus_tag="VBC28_002012"
     tRNA            complement(93..166)
                     /locus_tag="VBC28_002012"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(130..132),aa:Met,seq:cat)
     gene            complement(182..255)
                     /locus_tag="VBC28_002013"
     tRNA            complement(182..255)
                     /locus_tag="VBC28_002013"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(219..221),aa:Pro,seq:tgg)
     gene            complement(263..336)
                     /locus_tag="VBC28_002014"
     tRNA            complement(263..336)
                     /locus_tag="VBC28_002014"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(300..302),aa:Arg,seq:acg)
     gene            complement(348..432)
                     /locus_tag="VBC28_002015"
     tRNA            complement(348..432)
                     /locus_tag="VBC28_002015"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(396..398),aa:Leu,seq:taa)
     gene            complement(441..512)
                     /locus_tag="VBC28_002016"
     tRNA            complement(441..512)
                     /locus_tag="VBC28_002016"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(478..480),aa:Gly,seq:gcc)
BASE COUNT          150 a          153 c          122 g          116 t
ORIGIN      
        1 ggaccttgta ggactcgaac ctacgaccgc tcggttatga gccgagtgct ctaaccagtt
       61 gagctaaagg tccaaagtct caataaaata aatagcggcg gaggggatcg aacccccgac
      121 ctcccgggta tgaaccggac gctctagcca gctgagctac accgccataa aaatatatcc
      181 atcgggaaga caggattcga acctgcgaca ccttggtccc aaaccaagta ctctaccaag
      241 ctgagctact tcccgaaaaa tatgcaccct agaggagtcg aacctctaac cgcctgattc
      301 gtagtcaggt actctatcca gttgagctaa gggtgctaaa tattatatgc cgaggaccgg
      361 aatcgaaccg gtacgatgtt taccatcgca ggattttaag tcctgtgcgt ctgccagttc
      421 cgccaccccg gcctctaaca agcgaacgac ggggttcgaa cccgcgaccc tcaccttggc
      481 aaggtgatgt tctaccactg aactacgttc gcactaaaga cactatttat cctataaaat
      541 t
//
LOCUS       DATRMA010000162          456 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.180, whole genome shotgun sequence.
ACCESSION   DATRMA010000162 DATRMA010000000
VERSION     DATRMA010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..456
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.180"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            complement(<1..>456)
                     /locus_tag="VBC28_002017"
     CDS             complement(<1..>456)
                     /locus_tag="VBC28_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO6815947.1"
                     /translation="SDYDSWYTSFMNRGPSLTPFDDGILDMSMCDINWFKNLVNLQRK
                     EWESDIVYIFGVFLKSGEHVGMLNIVTLARADMQWGELGYVFHNQFWSNGYAFESILA
                     LLNSTYEKLGFHHIEAQITPGNERSEKLVRRLGLTYETTRKDFSFENGK"
BASE COUNT          155 a          102 c           47 g          152 t
ORIGIN      
        1 cattttccat tctcaaatga aaaatctttt cgagttgtct cataggttaa tcccaatctt
       61 ctaaccaact tttccgatcg ttcattccca ggagtaatct gtgcttcaat atgatgaaaa
      121 cccaattttt cataagtact attaagtaat gccaagattg actcaaatgc atagccatta
      181 ctccaaaatt gattatgaaa aacatatccc aattctcccc attgcatatc tgctcttgca
      241 agggtaacaa tatttaacat tcctacgtgc tcaccgcttt ttagaaaaac accaaatata
      301 tatactatgt cactttccca ttctttcctt tgtaaattta ctaaattttt aaaccaatta
      361 atgtcacaca tagacatatc aagtatccca tcatcaaatg gagttaaaga tggtccacga
      421 ttcataaaac tagtatacca actatcataa tcactt
//
LOCUS       DATRMA010000163          372 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.denovo.189, whole genome shotgun sequence.
ACCESSION   DATRMA010000163 DATRMA010000000
VERSION     DATRMA010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..372
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.denovo.189"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            complement(<1..>372)
                     /locus_tag="VBC28_002018"
     CDS             complement(<1..>372)
                     /locus_tag="VBC28_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HEO6815948.1"
                     /translation="LPLINLIQIYKRSQENLSFSKAIFLKKENYQRPFIIGISGSVAV
                     GKSTTSRLLQLLISRTFKDSHVELVTTDGFLYPNEKLIQNGILNRKGFPESYDMESLL
                     NFLDTIKNGITAKIPIYSHEIY"
BASE COUNT          124 a           48 c           70 g          130 t
ORIGIN      
        1 gtagatttca tgagaataaa ttgggatttt cgcggttata ccgttcttta tagtatctaa
       61 gaaatttaga agtgactcca tatcatagga ttccgggaaa cctttacggt ttaaaattcc
      121 attttgtatg agtttctcgt taggatataa aaagccatca gtagtgacta attcgacgtg
      181 actatcttta aaagttcgtg aaattagtaa ttgtagtaag cgactggtag ttgattttcc
      241 aacagcaact gaccctgata tgccgataat aaaaggacgt tgataatttt cctttttaag
      301 aaatattgct ttagaaaaag ataaattttc ttgactacgt ttatagattt gaataaggtt
      361 aattaaagga ag
//
LOCUS       DATRMA010000164         6279 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699859-rid18552493.guided.088, whole genome shotgun sequence.
ACCESSION   DATRMA010000164 DATRMA010000000
VERSION     DATRMA010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699859
            Sequence Read Archive: SRR7789299
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790159.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790159.1
            Genome Coverage       :: 166x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:36:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,991
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 27 of 47
            Pseudo Genes (incomplete)         :: 13 of 47
            Pseudo Genes (internal stop)      :: 13 of 47
            Pseudo Genes (multiple problems)  :: 5 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6279
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699859-rid18552493.guided.088"
                     /serotype="II"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2016-05-25"
                     /collected_by="RMPRU"
     gene            153..269
                     /locus_tag="VBC28_002019"
     CDS             153..269
                     /locus_tag="VBC28_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HEO6815949.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
     gene            285..2204
                     /gene="tet(M)"
                     /locus_tag="VBC28_002020"
     CDS             285..2204
                     /gene="tet(M)"
                     /locus_tag="VBC28_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HEO6815950.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFHNCSLFPVYH
                     GSAKNNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            2323..2490
                     /locus_tag="VBC28_002021"
     CDS             2323..2490
                     /locus_tag="VBC28_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HEO6815951.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(2550..2903)
                     /locus_tag="VBC28_002022"
     CDS             complement(2550..2903)
                     /locus_tag="VBC28_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO6815952.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLM
                     ESLASGINEARNIED"
     gene            3408..3830
                     /locus_tag="VBC28_002023"
     CDS             3408..3830
                     /locus_tag="VBC28_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEO6815953.1"
                     /translation="MKPSSFQTTIENQFDYICKRAMEDERKNYMLYLSRIAKREVSFS
                     DVGDYLVSQFATTDNYSTDFQIFTLNGLSVGVENDLLSEALRELPDKKREILLLFYFM
                     DMSDSEIADLLKLNRSTVYRHRTSGLALIKKFMEEFEE"
     gene            3827..4057
                     /locus_tag="VBC28_002024"
     CDS             3827..4057
                     /locus_tag="VBC28_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO6815954.1"
                     /translation="MKTQYPMIPFPLIVKATDGDTEAINQILHHYRGYITKRSLRLMK
                     DEYGNQSMVVDEVLRGRMETRLITKILSFEIK"
     gene            4518..4721
                     /locus_tag="VBC28_002025"
     CDS             4518..4721
                     /locus_tag="VBC28_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006730246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HEO6815955.1"
                     /translation="MKQTDIPIWERYTLTIEEASKYFRIGENKLRRLAEENKNANWLI
                     MNGNRIQIKRKQFEKIIDTLDAI"
     gene            4803..6020
                     /locus_tag="VBC28_002026"
     CDS             4803..6020
                     /locus_tag="VBC28_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020220015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEO6815956.1"
                     /translation="MSEKRRDNKGRILKTGESQRKDGRYLYKYIDSFGEPQFVYSWKL
                     VATDRVPAGKRDCISLREKIAELQKDIHDGIDVVGKKMTLCQLYAKQNAQRPKVRKNT
                     ETGRKYLMDILKKDKLGVRSIDSIKPSDAKEWAIRMSENGYAYQTINNYKRSLKASFY
                     IAIQDDCVRKNPFDFQLKAVLDDDTVPKTVLTEEQEEKLLAFAKADKTYSKNYDEILI
                     LLKTGLRISEFGGLTLPDLDFENRLVNIDHQLLRDTEIGYYIETPKTKSGERQVPMVE
                     EAYQAFKRVLANRKNDKRVEIDGYSDFLFLNRKNYPKVASDYNGMMKGLVKKYNKYNE
                     DKLPHITPHSLRHTFCTNYANAGMNPKALQYIMGHANIAMTLNYYAHATFDSAMAEMK
                     RLNKEKQQERLVA"
BASE COUNT         2195 a         1005 c         1231 g         1848 t
ORIGIN      
        1 tattatttaa gtgactattt aaaaggagtt aataaatatg cggcaaggta ttcttaaata
       61 aactgtcaat ttgatagcgg gaacaaataa ttagatgtcc ttttttagga gggcttagtt
      121 ttttgtaccc agtttaagaa tacctttatc atgtgattct aaagtatcca gagaatatct
      181 gtatgctttg tatacctatg gttatgcata aaaatcccag tgataaaagt atttatcact
      241 gggattttta tgcccttttg ggtttttgaa tggaggaaaa tcacatgaaa attattaata
      301 ttggagtttt agctcatgtt gatgcaggaa aaactacctt aacagaaagc ttattatata
      361 acagtggagc gattacagaa ttaggaagcg tggacaaagg tacaacgagg acggataata
      421 cgcttttaga acgtcagaga ggaattacaa ttcagacagg aataacctct tttcagtggg
      481 aaaatacgaa ggtgaacatc atagacacgc caggacatat ggatttctta gcagaagtat
      541 atcgttcatt atcagtttta gatggggcaa ttctactgat ttctgcaaaa gatggcgtac
      601 aagcacaaac tcgtatatta tttcatgcac ttaggaaaat ggggattccc acaatctttt
      661 ttatcaataa gattgaccaa aatggaattg atttatcaac ggtttatcag gatattaaag
      721 agaaactttc tgccgaaatt gtaatcaaac agaaggtaga actgtatcct aatatgtgtg
      781 tgacgaactt taccgaatct gaacaatggg atacggtaat agagggaaac gatgaccttt
      841 tagagaaata tatgtccggt aaatcattag aagcattgga actcgaacaa gaggaaagca
      901 taagatttca taattgttcc ctgttccctg tttatcacgg aagtgcaaaa aacaatatag
      961 ggattgataa ccttatagaa gtgattacga ataaatttta ttcatcaaca catcgaggtc
     1021 cgtctgaact ttgcggaaat gttttcaaaa ttgaatatac aaaaaaaaga caacgtcttg
     1081 catatatacg cctttatagt ggagtactac atttacgaga ttcggttaga gtatcagaaa
     1141 aagaaaaaat aaaagttaca gaaatgtata cttcaataaa tggtgaatta tgtaagattg
     1201 atagagctta ttctggagaa attgttattt tgcaaaatga gtttttgaag ttaaatagtg
     1261 ttcttggaga tacaaaacta ttgccacaga gaaaaaagat tgaaaatccg caccctctac
     1321 tacaaacaac tgttgaaccg agtaaacctg aacagagaga aatgttgctt gatgcccttt
     1381 tggaaatctc agatagtgat ccgcttctac gatattacgt ggattctacg acacatgaaa
     1441 ttatactttc tttcttaggg aaagtacaaa tggaagtgat tagtgcactg ttgcaagaaa
     1501 agtatcatgt ggagatagaa ctaaaagagc ctacagtcat ttatatggag agaccgttaa
     1561 aaaatgcaga atataccatt cacatcgaag tgccgccaaa tcctttctgg gcttccattg
     1621 gtttatctgt atcaccgctt ccgttgggaa gtggaatgca gtatgagagc tcggtttctc
     1681 ttggatactt aaatcaatca tttcaaaatg cagttatgga agggatacgc tatggttgcg
     1741 aacaaggatt atatggttgg aatgtgacgg attgtaaaat ctgttttaag tatggcttat
     1801 actatagccc tgttagtacc ccagcagatt ttcggatgct tgctcctatt gtattggaac
     1861 aagtcttaaa aaaagctgga acagaattgt tagagccata tcttagtttt aaaatttatg
     1921 cgccacagga atatctttca cgagcataca acgatgctcc taaatattgt gcgaacatcg
     1981 tagacactca attgaaaaat aatgaggtca ttcttagtgg agaaatccct gctcggtgta
     2041 ttcaagaata tcgtagtgat ttaactttct ttacaaatgg acgtagtgtt tgtttaacag
     2101 agttaaaagg gtaccatgtt actaccggtg aacctgtttg ccagccccgt cgtccaaata
     2161 gtcggataga taaagtacga tatatgttca ataaaataac ttagtgtatt ttatgttgtt
     2221 atataaatat ggtttcttgt taaataagat gaaatatttt ttaataaaga tttgaattaa
     2281 agtgtaaagg aggagatagt tattataaac tacaagtgga tattgtgtcc tgtatgtgga
     2341 aataaaacac gattaaagat aagggaagat actgaattaa aaaaattccc cctctattgt
     2401 ccgaaatgca gacaagaaaa tttaattgaa ataaagcagt tcaaagtaac tgtgattaca
     2461 gagccagacg caaagacgca gagccgataa aatgagatta atacaatctc attttatcgg
     2521 ctctttccgt tatgtatgga ttcttttaat tagtcttcga tgtttcttgc ttcgttgata
     2581 ccgctggcta aagattccat taaggatagt tctttgtctg taaagctatc catgtatttc
     2641 tctatctgta atcgtcgggt gctttttacc aagttattag caggtaagaa aaattcatca
     2701 acggaaacat gaagtaacga tacaaggtca taaagaactt gtatgctggg gtgttgccct
     2761 ttattttcaa tattagttaa gtaccgtggg tcaatttcaa tcaatgctcc cacttgttca
     2821 cgagttaaac ctcgtttcaa tcgagcttct ttaatggcta aaccaaaggc tctaaaatca
     2881 tatttatctt cttttttacg catagtagac cacctctata cattttattg ttcctactga
     2941 attaaaaaca ggtatagaaa aacgtgttat atggtttata ggtttatatt taataaaaag
     3001 cactactaaa cgccaataaa aaaaaccgtt atatggtagt gctatttacg ctgttaaaat
     3061 attgtatatt acttccaaat ggcggtttgt tggaggtcaa cgtcgccatg aagtacatca
     3121 tatacaataa atttccttac attgggttct tgtcaaaaaa agtcgtctat ctgcaataga
     3181 taagtacgtc caccaatgtg gttttataaa tcatatagat agaataacag aagcatgtaa
     3241 acagagaaat aaatctgttt atatgctttt ttggctattc agaacttttt tacaaagttt
     3301 atttatcagt aatgcaacaa atcccccttt cacattggga ctaagagtga aaggagataa
     3361 acgagcaagg ctcacttcct ttcctagaca gaaagggggt gagaaacatg aaaccatctt
     3421 cttttcagac cacaatagaa aatcagtttg actatatctg taaacgtgct atggaagacg
     3481 agcgaaagaa ttatatgctt tatctttcaa ggattgcaaa gcgtgaggtg tccttttcgg
     3541 atgttggcga ttatcttgtt agccagtttg cgacaacaga taactattca actgactttc
     3601 agatttttac actcaatggg ttatcagtag gcgttgaaaa tgatttgttg agtgaagcat
     3661 tacgtgagtt gccagacaag aaacgtgaaa ttctactgct gttttacttt atggacatga
     3721 gcgattcaga aattgcagac ctgttgaaat tgaaccgttc tactgtctat cggcatagaa
     3781 ccagtggact agccttaatt aaaaagttta tggaggaatt tgaagaatga aaacacaata
     3841 tcctatgatt ccctttcctc tcattgtaaa ggcaacagat ggcgataccg aagcgattaa
     3901 ccagattcta catcattaca gagggtacat aacgaagcgt tccctacgac ttatgaaaga
     3961 tgaatatggc aatcaaagta tggtcgttga tgaagtctta cgtggaagaa tggaaaccag
     4021 actgattaca aagattttgt catttgaaat taagtaatat cctctctcct ttcgtggaag
     4081 cgtgctaaac cattccacgc ttcccgaaca gggaggtttg ttattccacc aaagcatatt
     4141 gagctttcaa tgtgttttga taggctaacg agccattgtt ctttgaaaac tgaataaaag
     4201 taatcgaata cgtttcgata agaaaagagc caacggaact aaccgccatg acctatctta
     4261 taaagatagc gagcgattca tgttagtgat ccgagaagca atctttagca ggattgcctg
     4321 caacgacatt cttatcgtga taatgatact cccatacagt caatagtccg agcgtgataa
     4381 aaccgtcgca ggcaatgagt atggctacat gagaaccatg caggggtgga actcccgtga
     4441 gctttgctaa agctgttcga ttgctggtaa aacaactttt atgaaatcca aataagtgat
     4501 ttggaaagga ggattttatg aagcagactg acattcctat ttgggaacgt tataccctaa
     4561 ccattgaaga agcgtcaaaa tattttcgta ttggcgaaaa caagctacga cgcttggcag
     4621 aggaaaataa aaatgcaaat tggctgatta tgaatggcaa tcgtattcag attaaacgaa
     4681 aacaatttga aaaaattata gatacattgg acgcaatcta gcgtcgccaa agggtcttgt
     4741 atatgataaa atagtattaa gtcgtatcaa ggctctttcc ataaaggaaa ggagcaaatg
     4801 ccatgtcaga aaaaagacgt gacaataaag gtcgaatctt aaagactgga gagagccaac
     4861 gaaaagacgg aagatactta tacaaatata tagattcatt tggagaaccg caatttgttt
     4921 actcgtggaa acttgtggct acagaccgag taccagcagg aaagcgtgat tgtatctcac
     4981 ttagagagaa aatcgcagag ttacagaaag acattcatga tggtattgat gttgtaggaa
     5041 agaaaatgac actctgccag ctttacgcaa aacagaacgc tcaaagacca aaggttagaa
     5101 aaaacactga aactggacgc aaatatctta tggatatttt gaagaaagac aagttaggtg
     5161 taagaagtat tgacagtatt aagccatcag acgctaaaga atgggctatt agaatgagtg
     5221 aaaatggtta tgcttatcaa accatcaata actacaaacg ttctttaaag gcttcattct
     5281 atattgctat acaagatgat tgtgttcgga agaatccatt tgactttcaa ctgaaagcag
     5341 ttcttgatga tgatactgtc cctaagaccg tactaacaga agaacaggaa gaaaaactgt
     5401 tagcctttgc aaaagctgat aaaacctaca gcaaaaatta tgatgaaatt ctgatactct
     5461 taaaaacagg tcttcgtatt tcagagtttg gtggtttgac acttccagat ttagattttg
     5521 agaatcgtct tgtcaatata gaccatcagc tattgagaga tactgaaatt gggtactaca
     5581 ttgaaacacc aaagaccaaa agtggcgaac gtcaagttcc tatggttgaa gaagcctatc
     5641 aagcatttaa gcgagtgtta gcgaatcgaa agaatgataa gcgtgttgag attgatggat
     5701 atagtgattt cctctttctt aatagaaaga actatccaaa agtggcaagt gattacaacg
     5761 gcatgatgaa aggtcttgtt aagaaataca ataagtataa cgaggataaa ttgccacaca
     5821 tcactccaca tagtttgcga catacattct gtaccaacta tgcaaatgca ggaatgaatc
     5881 caaaggcatt acagtacatt atgggacatg ctaatatagc catgacgctg aactattacg
     5941 cacatgcaac attcgattct gcaatggcag aaatgaaacg cttgaataaa gagaagcaac
     6001 aggagcgtct tgttgcttag tagtacaaat gaatttacta cttatttacc acttctgaca
     6061 gctaagacat gaggaaatat gcaaagaaac gtgaagtatc ttcctacagt aaaaatactc
     6121 gaaagcacat agaataaggc tttacgagca tttaagaaaa tataaaaaga taattagaaa
     6181 tttatacttt gtttgtttat aaaaattaat aaagaaagat aaattttcta gaacaaatct
     6241 agaacaaaaa taagttattg aagaaataca agatatacc
//
